v>EPA

       U.S. Environmental
        Protection Agency
    Office of Solid Waste and
      Emergency Response
        Office of Research
        and Development
                          EP/V540/N-95/500   No. 12  August 1995
    iwmmms
                       IN THE FIELD
                An information update on applying bioremediation to site cleanup.
                      remediation
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In This Issue
Update on the Bioremediation Field Initiative	
EPA To Release Proposed Hazardous Waste Identification
    Rule for Contaminated Media	
Bioremediation in the Field Search System (BFSS)
    Version 2.0 Released 	
                    1

                    1

                    4
                    5
Updated Data Show Bioremediation Trends	
Preliminary Risk Assessment for Bioremediation
    Microorganisms	6
Cooperative Research and Development Agreement To
    Address Bioremediation of Chlorinated Solvents	8
EPA Publishes Proposed Rules for Microorganisms Under
    TSCA Section 5 	9
ORD Laboratories Restructured To Strengthen EPA's
   Research Focus	
EPA Bioremediation Publications  . .
Field Applications of Bioremediation
                  .11
                  .12
                  .14
                                    EPA To Release
                                    Proposed
                                    Hazardous Waste
                                    Identification Rule
                                    for Contaminated
                                    Media
In the fall of 1995, EPA plans to release for comment
the proposed Hazardous Waste Identification Rule for
Contaminated Media (HWIR-media). The HWIR-
media rule is a regulatory reform proposal that will
reexamine many of the RCRASubtitle C treatment and
management standards for contaminated media man-
aged in cleanups overseen by EPA or authorized states.
This proposed rule will address the major RCRA Sub-
title C management requirements, which are consid-
ered the biggest causes of problems for  cleanups.
These requirements include the Land Disposal Restric-
tions (LDRs), Minimum  Technical  Requirements
(MTRs), and RCRA permitting procedures.
To develop the framework for this proposal, EPA de-
cided to work with state regulators as "co-regulators"
and jointly develop the new provisions. Because the
RCRA program is delegated to the states, it will gen-
erally be the state regulators who will be implement-
ing the new requirements. In addition, EPA and the
states formed a Hazardous Waste Dialogue Commit-
tee chartered under the Federal Advisory Committee
Act (FACA), referred to as the "HWIR FACA Commit-
tee." This committee was made up of representatives
from EPA, states, environmental groups, the hazard-
ous waste treatment industry, and regulated industry.
EPA and the states asked the committee to discuss the
issues and make recommendations as to what the new
requirements for contaminated media should be.  The
FACA committee met between January 1993 and Sep-
tember 1994.
Of course, different members of the committee  had
different priorities and opinions about how the rule
should be structured and what the new requirements
should be. The resulting format that the committee
eventually recommended was a compromise between
                        (Continued on page 4)
Printed on Recycled Paper

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Bioremediation in the Field
T""r"""                         n •**• 2 *^ ^> %*>£**•% *#\ *•"*> V ™ •>* *'   f      *    '  /    "  '"  y *         "    }
  Update on the Bioremediation Field initiative    ,  to asgegs ^ hydrocarbon contaminationremaining
 {"'(Continued frontpage'ft * **" ^^*te*^wW*' -^HMelaV after treatment, and modeling to predict
           J    ^°              >-         *   -«-  whejflhierc a plume of contamination would return
                                                   after active remediation was discontinued,
                                                        ^              *    /
                                                   RSKBRL collected aquifer core samples to deter-
  Denver, Colorado; and Reilly Tar an
   oration Superfund site, St. Louis Park, Minnesota
   ecent results from four of these sjttes are presented    Jnjtne the quantity and* distribution of hydrocarbons
      '        '
^.ft,fili-*W#'t!	ji:vWTi*":"-Wfffittlii™«.>-i   roTOii_water flow Wdelin  to determine the ve-
  ,.-     ,. ^    .-    ,   j _   .    .    ..  /T-TTTS     ground-water flow modeling to determine the ve-
  bioremediation in  a lan^d, toatoent  umt  (LTU),  ';,fodty'and  trajectory  of potential'contaminant
  |r6und-water treatment in a^aboveg^omaf^ed^  '   ,^ TheresUchteam recommended that com-
  i^,S10^to^,a^,^.^^a8^^A^- ., ^>  JT   ""^ ^   •*-  ••*-    /  /     */
   .ioremediation of each 9-in. lift of sofljppMed to the  ^ • and that compliance wells be designed to minimize
  _JIJ required 32 to 16§"daysi Downward migration  '  ' cpntaminant dilution, A report on this work will be
f|' oif target chemicals  as a result of the application of     available later this-year; the next bulletin will ex-
   dditi9nal lifts was not observed, and^cpntmnin^ed^^, <>4plain how to obtain a copy.
   "*il was detoxified to1?ac¥gTca;tfS*le^'eI^aTa^Silt<
  	i=ii__	i(ti|e	treatment. ^Performance^^f ttie "fixed^film'
  Sioreactors''mdicate's''that* efrectiveTK4Jl ancl':
  ,,11!:,,'!	i	:»iii,ii, 	|,'i|	'•	i»' 	nr i'	a"	M»	liU'mniiii .«!	Bpwim !!ii»|ih«i*Mw w i IP t* fWJSPfiifW^S'BfjW-  	
  L jj^^t'aj, pccurj^^.ana jmat removal e^
if:'a function of loading rate anS'reteniSoit
                                                    *Rei«y Tar and Chemicai Corporation
                                                    '**   ^ '   -* Site, St. Louis Parkj Minnesota
                                        B" t£"* f i  r
                                       ^. In situ
 I treatment was observed in the water phase un^r
p the influence of the injectibn system; however/con-,
  tamination was present as nonaqueous phase liquid
  (NAPL). Reduced inorganic compounds present in
  ! the water phase and degassing of hydrogen petox-
 I Ide within the soil indicated abiotic loss pathways'
 fti\      »        j   * jj.'SiJH,!,1* ^ a1 ift* 3?™^ iwsiK'^ " *  <~ 4^* -* ' *
  j for the oxygen that was injected to stimulate biore-
  f'j^ediation. A report on this work will^be available
  later this year; trie next bulletin will,expla» how to
  obtain a copy.

 I; Public Service Company, b
 ,| ^m 1989, to 1992, ah'M situ'b	_..5__T.,^_.,1^	T^
 i' was ^operated at  the Public Service Company of
 'f j^Ipiado.fPSC^site in,Denver where uswed ojl hadj'
  |leal*!p -f-
            nutrient and  hydrogen peroxide addi->
             i.   "  'i             - ,-v'.jrg"i-.f"'3T '"^-wr,.'^-" TS^'
   In July 1992, the Robert S. Kerr EnvironmentaH•-.' .,-.-. -  . -  . ,  .  ..,  *  ,<-. JCpqtinuedwpage3)

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                                                                                Bioremediation in the Field
 f^*^^
 ^1?*rfHt^'#&K^$^r?:H'£a^
 ->,,4w,4-a^-^°K:,w<-x«rf*^;vi^^^

|^Jfi^^|}^es^^|r3(fi
 The Bioremediation Field Initiative is a cooperative effort among the Technology Innovation Office (TIO)
 Office of Solid Waste and Emergency Response (OSWER), and the National Risk Management Research
 Laboratory (NRMRL), Office of Research and Development (ORD). Major  contributors to the Initiative
 include the waste programs in the EPA Regional Offices and the following laboratories in ORD: Ada, OK-
 Athens, GA; Cincinnati, OH; Gulf Breeze, PL; and Research Triangle Park, NC.

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Bloremedlatlon In the Field
                              EPA's  Bioremedia-
                              tion Field Initiative
                              is pleased to  an-
                              nounce the release
                              of Version 2,0 of the
                              Bioremediation  in
                              the Field SearchSys-
                              tem (BFSS). BFSS is
                             •a* PC-basecl  .soft-
  ware product that provides access to a database of
  information on waste sites in the United States and
  Canada where bioremediation is being tested or
  implemented, or has been completed. Version 2.0 of
  BFSS contains information on more than 450 sites
  being tracked by the Bioremediatibn Field "Initiative,
  compared with 160 sites in the previous version. In
  the past, site information has been obtained solely
  from government sources. The new update includes
  information from public and private sources. BFSS
  allows users  to search the database electronically.
  View data on specific types of bioremediation sites^
  and print reports of selected information. Users can
  obtain information about the site location, media,
  "Contaminants, treatment technologies, costs,  and
   >erformance.
Bioremediation in the Field Search
System (BFSS) Version 2.0 Released

                     BFSS Version 2^0 contains ^several modifications to,/
                     enhance its usefulness. Version 2.0 provides:
                     • Capability to search by country.,      ,  •
                     • Capability to search by site use/characterizattort
                      (such as petroleum, solvents, woodpreserving) as,
                      well as by specific contaminants.
                     • Capability  to search for BTEX and carcinogenic
                      ''
                     * Addition'of a broader soils/sediments classification.
                     BFSS is available on EPA's Alternative Treatment
                     Technology, Information Clearinghouse '(ATTIC)
                     (703-589-8366),  Cleanup ' Information, (CLU-IN)
                     (301-589-8366), and Office of Research and De-
                     velopment (ORD) (513-569-7610) electronic bulletin
                     board systems. The software is designed. to be'
                     downloaded and operated off of a hard disk or a
                     local,area network (LAN). BFSS is also available on
                     diskette from EPA's National Risk  Management
                    ' Research Laboratory, Technology Transfer and Sup-
                     port Division, Technology Transfer Branch. To re-
                     quest a copy of the system, call 513-569r7562. Ask
                     for EPA/R-95/508a for the software manual and
                    •EPA/540/R-95/508b for the software.
 EPA To Release Proposed Hazardous Waste
 Identification Rule for Contaminated Media

 (Continued from page 2)

 the environmental community's preference for nation-
 ally  consistent standards and regulated industry's
 preference for site-specific flexibility.

 The  compromise format called the "Harmonized Ap-
 proach" was based first on the premise that only clean-
 ups  overseen by EPA or authorized states would be
 eligible for the flexibility of this new rule. This is
 important because many of the new requirements de-
 pend on the  judgment of a responsible overseeing
 Agency (EPA or the state). The approach divides more
 highly contaminated media from less contaminated
 media, and regulates them differently. Overseeing
 agencies are given the opportunity to exempt the less
 contaminated media from Subtitle C management re-
 quirements and set new requirements based on site-
 specific conditions or  concerns. The  more highly
 contaminated media remain subject to Subtitle C, but
 this  rule modifies the  applicable  permitting, treat-
 ment, and management requirements. A "bright line"
 of constituent-specific concentrations will be intro-
                     duced to limit the discretion of the overseeing Agency
                     in exempting media from Subtitle C.

                     Specific to bioremediation, this rule will propose to
                     introduce regulatory flexibility for both the more
                     highly contaminated media and the less contaminated
                     media. For the media remaining subject to Subtitle C,
                     this rule will propose alternative treatment standards
                     to the current Universal Treatment Standards (UTS)
                     required under the RCRA land disposal restrictions,
                     and will allow for site-specific variances even of the
                     new standards where appropriate. Also, the Agency
                     will propose a much more streamlined mechanism for
                     the permitting cleanup activities. This new approach
                     will  attempt to expedite the permitting of remedial
                     activities for contaminated media. Finally, the oversee-
                     ing Agencies will have complete discretion to direct
                     site-specific appropriate standards for the media that
                     will be exempted from Subtitle C.

                     The proposed HWIR-media rule will solicit colftiments
                     on many different options for the management issues
                     that pertain to contaminated media. Since these issues
                     are very complicated and controversial, the Agency is
                     eagerly awaiting public comment on this proposal. For
                     further information on HWIR-media, contact Carolyn
                     Hoskinson at 703-308-8626.

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                                                                                 Bioremediation in the Field
                      Updated Data  Show Bioiremediation Trends
                     According to recently updated
                     data from the Bioremediation
                     in the Field Search System, soil
                     remains the medium most fre-
                     quently bioremediated (368 of
                     the  455  sites), followed by
                     ground water (204 sites), as
                     shown in Figure 1. Sediments,
                     sludge, surface water, and air
                     are  third, fourth,  fifth,  and
sixth, respectively. Bioremediation is used to treat
more than one medium at many sites.
  Field Initiative
      400

      350

      300

      250
    CO

   CO
   13
    5 200
   .a
    E
      150

      100

       50

        0
             368
                              15
                                     8
                                          Air
            Soil  Ground  Sedi- Sludge Surface
                 Water  ments       Water
Figure 1. Number of sites treating each medium. (Some sites treat
       more than one medium.)

Figure 2 shows the major categories of waste undergo-
ing bioremediation at sites in the database. Petroleum
is the target contaminant at by far the greatest number
of sites (313), followed by solvents (60 sites) and wood
preserving wastes (51 sites). Other target contami-
nants are grouped  in the categories of pesticides/
herbicides, munitions, and  "other." At some sites,
more than one contaminant type is undergoing biore-
mediation.
Figure 3 presents  the status of 322 biotreatment proc-
esses at the 309 sites for which information about the
    350

    300
  ^250
  •i
  CO
    200
          313
  .§150

  = 100

     50

      0
        Petroleum Solvents  Wood   Other Pesticides/ Muni-
                     Preserving      Herbicides tions
                      Wastes
Figure 2. Breakdown of sites by type of contamination.
                                                                                       166
                                          53
                                                         200
                                                       CO
                                                       8M50
                                                       CO
                                                       8
                                                      2:100
                                                       o
                                                         50
          Pre-      In     Installa-   Opera-   Corn-
         design   design    tion     tional    pleted
Figure 3. Stage of operation for processes for which information
       about the stage of full-scale operation is available.

stage of full-scale operation is available. Of these 322
processes, 166 (51 percent) are operational, and treat-
ment has been completed at 53 sites (16 percent).
Figure 4 shows a breakdown of sites by treatment
technology. In situ treatment is used or being consid-
ered at 313 of the sites in the database. Bioventing is
the most common in situ technology (108 sites), fol-
lowed by in situ ground-water bioremediation (103
sites) and in situ soil bioremediation (103  sites),  air
sparging (40 sites), intrinsic bioremediation (38 sites),
in situ sediment bioremediation (11 sites), and con-
fined treatment for sediments (3 sites). Other in situ
technologies are being considered-or used at 26 sites.
(Some sites use more than one in situ technology.) Ex
situ treatment without reactors (e.g., aerated lagoon,
land treatment, pile treatment/composting) is being
considered or used at 116 sites, while ex situ treatment
with  reactors  (e.g., attached  growth,  fixed  film,
fluidized bed, slurry reactor, sequencing batch reactor,
extended aeration) is being considered or used at 73
sites.
       350

     | 300
     co 250
     £ 200
     CD
     •f 150
     £ 100
        50
         0
                                                                     313
                                                                              116
                                                                                         73
                                                                   In Situ     Ex Situ    Ex Situ
                                                                             (without     (with
                                                                             reactor)    reactor)

                                                     Figure 4. Breakdown of sites by treatment technology. Includes
                                                             laboratory-, pilot-, and full-scale. (Some sites use more'
                                                             than one technology.)   '
                                                                                     (Continued on page 6) <

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Bloremedlation in the Field
                Preliminary
                Risk Assessment
                for Bioremediation
                Microorganisms
Since the discovery of polychlorinated biphenyls
(PCBs) as persistent environmental contaminants,
these compounds have  presented problems with re-
spect to environmental biodegradation and bioreme-
diation.  Biologically, the  vast  majority  of  PCB
congeners are not biochemically degraded by micro-
organisms; chemically, these relatively insoluble com-
pounds largely exist as  sorbed residues with limited
bioavailability.

Aerobic biodegradation of PCB congeners and com-
mercial mixtures has been documented in the environ-
ment and for pure cultures of some bacteria and fungi.
Aerobic bacterial degradation of PCBs appears to oc-
cur primarily through a co-metabolic pathway using
genetic operons and enzymes associated  with bi-
phenyl degradation. Recent evidence also  suggests
that PCBs may be slowly dechlorinated to less chlorin-
ated congeners by microbiological reduction of PCBs
as electron  acceptors.  If biological/chemical and
physical barriers to environmental PCB biodegrada-
tion can be overcome, many attractive applications for
bioremediation of PCBs exist, such as in the electrical
power generation and distribution industry.

GEM Development for Bioremediation of PCBs

The Tennessee Valley Authority (TVA) and the Electric
Power Research Institute (EPRI) are addressing this
problem by using biotechnology to develop and dem-
onstrate a new PCB bioremediation strategy. The strat-
egy is based on the use  of genetically engineered
microorganisms (GEMs) made competent for co-me-
tabolic biodegradation, and the use of surfactants to
increase bioavailable PCB concentrations and provide
a selective growth substrate for the organisms. The par-
ent organism used in this work is Pseudomonas  putida
(designated strain IPL5), in which a PCB degradation
phenotype was established by the introduction of a
biphenyl degradative operon as either a plasmid repli-
con or as a chromosomally based transposon insertion.

The proposed site for field testing of this strategy is a
restricted access site near Chattanooga, Tennessee. The
low-slope site is 1,000 ft from the nearest surface water.
The site has four electrical capacitor banks which were
                               (Continued on page 7)
Updated Data Show Bioremediation Trends

(Continued from page 5)

Figure 5 depicts the legislative authority under which
each site is categorized. As the figure shows, more than
one-third of the sites are Underground Storage Tank
(UST) sites; nearly one-fourth are Comprehensive En-
vironmental Response, Compensation, and Liability
Act (CERCLA) sites; and the remaining sites are Re-
source Conservation and Recovery Act (RCRA), Toxic
Substances Control Act (TSCA), Federal Facility, inter-
national, and "other" sites.
Figure 6 shows the wide geographical distribution of
the bioremediation sites. The sites are found in all 10
EPA regions and in Canada, but 40 percent of the sites
are located in EPA Region 5. A significant number of
sites using bioremediation are from the Michigan De-
partment of Natural Resources' Underground Storage
Tank Program.
                 International
                Private Sector
         International
         Government
          17 3.8%

            RCRA
            43 9.6%
              Other
             59 13.1%
                       Federal Facility
                        80 17.8%
                                                                  Canada 4.2% Region I 2.7%
                                                             Region X 2.7%  \   /   Region II 5.3%
   Region IX 15.8%



  Region VIII 6.7%

   Region VII 2.7%

    Region VI 4.9%
                                                                                  Region III 4.4%
Region IV 10.2%
                                                                                   Region V 40.4%
Figure 5. Legislative Authority of Bioremediation Sites.
Figure 6. Distribution of Bioremediation Projects by Region.

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                                                                                Bioremediation in the Field
 Preliminary Risk Assessment for Bioremediation Microorganisms

 (Continued from page 6)
 de-energized in 1992. Between 1971 and 1992, the PCB
 congeners Arochlor 1242 and 1248  leaked from  the
 capacitors into the gravel and soil below. Most of the
 testing on the site will  use  7,000 kg of gravel-soil
 interface material with PCB concentrations in the 5 to
 140 ppm range. Few native bacteria exist in the inter-
 face material, possibly due to its high pH. No other
 pollutants are present in the material.


 Bioremediation Process Design

 The PCB proposed bioremediation process design (see
 Figure 1) consists of a recirculatory bed system where
 surfactant is applied to the soil. Solubilized PCBs  are
 recovered as  leachate and  pumped to an  onsite
 bioreactor containing the PCB degradative strains.
 Surfactant degradation and PCB bioremediation  are
 accomplished primarily in the bioreactor. A concen-
 trated PCB residual is fed as  a side stream to an  an-
 aerobic bioreactor  for reductive dechlorination  of
 recalcitrant PCB with recycle back to the aerobic biore-
 actor. Effluent from the aerobic bioreactor containing
 makeup surfactant and GEMs is  then recycled in a
 surface application format to the treatment bed, where
 some anaerobic and aerobic PCB degradation takes
 place.
   Bioremediation Risk Assessment

   EPA has undertaken a  coordinated risk review of
   the P. putida IPL5 recombinant prior to field testing of
   the bacteria, as required under Sections 5 and 6 of the
   federal Toxic Substances Control Act (TSCA).  The
   Agency's Office of Pollution Prevention and Toxics
   (OPPT) conducted a partial risk assessment based on
   information submitted by TVA and the University of
   Tennessee. Figure 2 presents a draft OPPT risk assess-
   ment analysis scheme for bioremediation microorgan-
   isms. EPA's partial  risk  assessment  for P. putida
   primarily focused on pretest information and expo-
   sure information (see Steps 1 and 2 of Figure 2).

   Based on preliminary information, EPA has found that
   the overall risk of a field test using the P. putida recom-
   binant appears  to be low due to the nature of the
   microbial recipient and its introduced DNA, and due
   to the extent of containment provided by the experi-
   mental design. Additional information may be needed
   to further characterize the introduced DNA, estimate
   concentrations of process byproducts and transforma-
   tion  products (chlorobenzoates and nonylphenols),
                                  (Continued on page 8)
            Air,
            Nutrients,
            Organisms

            Diatomaceousl  Bjoreactor
            Earth
         Supplemental
         Physiochemical
         Biological
         Treatment
SOIL IRRIGATION GRID
                                                                            Collection Trench
Figure 1. Simplified design for field treatment of PCBs using a surfactant-based PCB solubilization with surfactant/PCB degrading.

                                                 7

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Bloremedlation in the Field
Preliminary Risk Assessment for
Bioremediation Microorganisms

(Continued from page 7)
and characterize potential worker exposure to micro-
organisms and metabolites.

For more information about this preliminary risk as-
sessment, see Gary S. Sayler and Philip Sayre, Prelimi-
nary Risk Assessment for  Recombinant Pseudomonads
Released Into the Environment for Hazardous Waste Deg-
radation, Proceedings of the OECD/DSTI Tokyo Work-
shop  on Bioremediation, November 27-30,  1994,
OECD, Paris (in press).
   Step 1 Risk Assessment Based on Pretest Information
   I. Organism Characterization
       Species (donor and recipient, consortia)
       Pathogenicity associated with species
       Metabolic pathway information
   II. Site Characterization
       Difference between on- and off-site characteristics
       Contaminants present
       Modes of mobility
   HI. Mfcroblal Interaction With Contaminant
       Likely transformation products
       Aqueous solubility of contaminant
       Interaction of contaminant with other contaminants on site
   IV. Application Characterization
       Engineering design for bioremediation
       Contaminant provided by design	
       Low
       Risk
   Step 2 Exposure
          Assessment
   I.  Migration
   II.  Fate Models
   III. Microcosm Tests for
      Mtorobial Growth/
      Perelstence
   IV. Microcosm Tests for
      Chemical Presence/
      Persistence
 Risk
Low
Risk
                 Risk
Step 3 Hazard
       Assessment
I. Quantitative Structure
    Activity Relationship
    Analysis
II. Pathogenicity of Related
    Strains
III. Toxicity Tests
IV. Pathogenicity Tests
V. Other Tests
  Step 4  Risk Characterization/Professional Judgment Used:
    Benefits Examined, Decision Made on Whether To Approve
    Bioremediation Application
         Approval Dependent on Monitoring Results
             Next Level Examined for Approval
                                               Cooperative Research
                                               and Development
                                               Agreement To
                                               Address
                                               Bioremediation of
                                               Chlorinated Solvents
In early August, a Cooperative Research and Develop-
ment Agreement (CRADA) will be signed by EPA's
National  Risk Management Research  Laboratory
(NRMRL), the U.S. Department of Energy (DOE), the
U.S. Department of Defense, and the following com-
panies: Ciba-Geigy, DuPont Chemicals, Dow Chemi-
cal,  General Electric, Monsanto, and Zeneca. This
agreement covers three areas associated with bioreme-
diation of chlorinated solvents: intrinsic bioremedia-
tion (or natural attenuation) of low concentrations of
chlorinated solvents in ground water, accelerated an-
aerobic biotreatment of higher concentrations of chlo-
rinated solvents in ground water, and co-metabolic
bioventing of residual chlorinated  solvents in the
vadose zone. A ceremony, including the vice presi-
dents of the companies, formalized  this agreement
during EPA's Bioremediation Symposium on August
8-10,1995, in Rye Brook, New York.

Members of this public-private consortium will col-
laborate  to  perform  the laboratory research, field
studies,  and modeling necessary to accelerate the
development of the most cost-effective in situ biore-
mediation processes for degradation of chlorinated
solvents  and to achieve public and regulatory accep-
tance of these technologies. Field testing of biore-
mediation methods for in situ bioremediation  is
scheduled to begin this summer at Dover  Air Force
Base in Delaware. Once the field tests are under way,
the consortium plans to conduct additional tests of the
three bioremediation processes at another site.
Figure 2. Draft risk assessment analysis scheme for bioremedia-
        tion microorganisms.
                                The success of these field tests and the development of
                                protocols for applying the technologies at other sites
                                will have far-reaching implications. Use of the three
                                processes being tested has  the potential to  greatly
                                reduce the $500 million that the government expects
                                to spend cleaning up sites contaminated with chlorin-
                                ated solvents. According to Jeff Walker, a program
                                manager in the DOE's Office of Technology Develop-
                                ment, "Bioremediation of ground water can reduce the
                                costs by an estimated 50 to 75 percent relative to the
                                conventional method of pumping  and treating the
                                water, and bioremediation of contaminated soils can
                                be up to 10 times  cheaper than traditional 'dig and
                                burn' technologies."

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                                                                             Bioremediation in the Field
                EPA Publishes
                Proposed Rules for
                Microorganisms
                Under TSCA
                Section 5
 The March 1992 issue of "Bioremediation in the Field"
 contained a discussion of EPA's draft proposed rules
 for microorganisms under the Toxic Substances Con-
 trol Act (TSCA). Those proposed rules were published
 for public comment in September 1994 under the title
 "Microbial Products  of Biotechnology; Proposed
 Regulation Under the Toxic Substances Control Act (59
 PR 45526, September I, 1994). The public comment
 period on the proposal closed on December 31,1994.
 EPAis analyzing the public comments and developing
 the final rule which should provide a complete frame-
 work for its program for microorganisms under TSCA.
 Until this proposed regulation is published as a final
 rule, EPA will continue to operate its TSCAbiotechnol-
 ogy program under a 1986 policy statement made as
 part of an interagency Coordinated Framework for
 Biotechnology (51 PR 23302, June 26, 1986).  In 1986,
 EPA indicated that it could implement part of its pro-
 gram for microorganisms based on the program al-
 ready in existence for traditional chemicals, but that
 the full program could  only be implemented after
 rulemaking. This article provides an update to the
 March 1992 discussion of the TSCA biotechnology
 program and the proposed rules that were then in
 draft form.

 Microorganisms Subject to TSCA
 TSCA authorizes EPA to regulate chemical substances,
 except for certain substances covered by other federal
 agencies. "Chemical substance" is defined in TSCA
 broadly enough to cover microorganisms. Under
 TSCA section 5, EPA requires submission of a notifica-
 tion (referred to as a premanufacture notification, or
 PMN) for a new chemical substance before it is manu-
 factured in commerce in the United States. Microor-
 ganisms will be subject to some obligations under
 TSCA section 5 if they meet all of the following: (1) they
 are manufactured for a use subject to TSCA, (2) they
 are "new" microorganisms, and (3) they are manufac-
 tured for commercial purposes.

 TSCA uses. Microorganisms  produced for environ-
mental, industrial, or consumer uses that are not spe-
cifically excluded from TSCA could potentially be
regulated under TSCA. TSCA should be of interest to
the bioremediation  community, because potential
TSCA uses of microorganisms include bioremediation
 of hazardous waste sites, enhanced oil recovery, and
 metal extraction and concentration.
 New microorganisms. The biggest difference in the pub-
 lished proposal and the draft discussed in March 1992
 is in the scope of microorganisms covered. EPA's cur-
 rent program, based on the 1986 policy statement,
 requires PMN reporting for "new" microorganisms,
 which are defined as intergeneric microorganisms (mi-
 croorganisms formed by deliberate combinations of
 genetic material from source organisms in different
 taxonornic genera). The draft rules proposed a differ-
 ent scope for new microorganisms. The 1994 publish-
 ed proposal, however, opted to retain the intergeneric
 interpretation. In the 1994 proposal, EPAindicated that
 it may decide to reconsider its interpretation of "new"
 microorganism at a later time and requested comment
 on  whether  alternative  interpretations of  "new"
 should be explored in a separate rulemaking.

 A key element of the intergeneric interpretation is that
 EPA does not  require PMN reporting under TSCA
 section 5 for naturally occurring microorganisms and
 for genetically modified microorganisms that are not
 intergeneric. These types of  microorganisms  are
 not  considered new and therefore are implicitly in-
 cluded on the TSCAInventory of Chemical Substances
 manufactured  in the United States. Because of the
 intergeneric interpretation, EPA has not seen many
 submissions under TSCA for microorganisms used for
 bioremediation. A few researchers in bioremediation
 are beginning to develop microorganisms that would
 meet EPA's intergeneric trigger.  These  researchers
 have been consulting EPA about the status of their
 microorganisms under TSCA.

 Commercial purposes.  The final consideration in deter-
 mining the status of microorganisms under TSCA is
 whether they are produced for commercial purposes.
 TSCA section 5 (i) limits section 5 coverage to chemical
 substances  produced  for  "commercial  purposes."
 While it is obvious when a microorganism is being
 produced for a commercial purpose after the research
 and  development (R&D) stage, it is more difficult to
 determine what constitutes commercial R&D. All re-
 search conducted directly by a commercial entity is
 considered to be for commercial purposes. Determin-
 ing what is commercial R&D, however,  has been a
 continuing challenge for the TSCAbiotechnology pro-
 gram, because university and industry relationships
 have become increasingly complex for biotechnology.
 EPA  discussed  three possible interpretations in the
proposed rules and will consider the public comments
received before developing its interpretation for the
final rule. The 1986 policy statement uses an interpre-
tation that looks primarily at the funding source. For
example, research that is being conducted  at a univer-
sity but directly funded by a business would be con-
                             (Continued on page 10)

-------
Bloremedlatlon in the Field
EPA Publishes Proposed Rules for Microorganisms Under TSCA Section 5
(Continued from page 9)                     	
sidered to be for commercial purposes. Persons who
are unsure as to whether their research would be con-
sidered to be for commercial purposes are encouraged
to contact EPA.

Obligations Under TSCA Section 5
The commercial purposes consideration has had little
practical effect on research using traditional chemicals,
because most of that research is exempt from PMN
reporting. TSCA section 5(h)(3) allows EPA to exempt
R&D activities involving chemical  substances pro-
duced in small quantities. While a  small quantities
definition can be developed for chemicals used in
discrete amounts, it cannot be applied with the same
expectations to living microorganisms, which have the
ability to reproduce and spread. In 1986, EPAindicated
that it would apply the small quantities exemption to
new microorganisms used in R&D activities in con-
tained structures that were designed to minimize un-
intentional releases  of  microorganisms.  EPA also
indicated, however, that because of greater uncer-
tainty, researchers using new microorganisms in R&D
activities involving intentional environmental release
would be encouraged to voluntarily submit PMNs for
these microorganisms until EPA could develop a spe-
cial reporting program for environmental releases.

R&D exemption. The proposed rules contain an R&D
exemption for microorganisms used in  contained
structures, with structures defined broadly enough to
encompass greenhouses and bioreactors. The exempt
R&D activities must be conducted under the supervi-
sion of a technically qualified individual (TQI) who is
responsible for selecting appropriate containment and
inactivation for the microorganisms. Under the 1986
policy and the proposed rules, researchers are  ex-
pected to determine whether their R&D activities are
conducted under conditions that meet the contained
structures requirements. Researchers who are uncer-
tain may contact EPA to discuss their work. In the past
few years, some researchers working with intergeneric
microorganisms  for bioremediation have contacted
EPA about the status of their bioreactors, and EPA has
concluded that these bioreactors were eligible for the
R&D exemption.
      reporting. The proposed rules contain an abbre-
 viated reporting process for R&D releases called the
 TSCAExperimental Release Application (TERA). This
 involves a 60-day review period focusing strictly on
 the proposed experiment.  In conjunction with the
 TERA, EPA has proposed a TERA exemption process
 for specific microorganisms with which it has suffi-
 cient familiarity, either through previous reviews or
other information submitted by researchers. The pro-
posal also discusses a low risk alternative for microor-
ganisms meeting certain criteria. Under the current
program, researchers are urged to submit voluntary
PMNs. EPA is in the process of reviewing two submis-
sions for environmental tests involving intergeneric
microorganisms used for bioremediation.

Reporting beyond  R&D. Once  a microorganism has
moved beyond the R&D stage to what EPA calls "gen-
eral commercial use/' full reporting is required. In the
proposed rules, EPA has developed data requirements
specifically for microorganisms. These would be sub-
mitted in the Microbial Commercial Activity Notice
(MCAN) for a 90-day review by EPA. Under the cur-
rent program, persons submit PMNs for microorgan-
isms and use  guidance found in EPA's "Points to
Consider in the Preparation and Submission of TSCA
Premanufacture Notifications (PMNs) for Microor-
ganisms." EPA has reviewed PMNs for commerciali-
zation of microorganisms  used as intermediates in
fermentation systems to produce specialty chemicals.
EPA has not yet approved a PMN for commercializa-
tion of an intergeneric microorganism for intentional
environmental release, however, although a request to
commercialize an intergeneric strain of a bacterium for
nitrogen fixation is under consideration. Intergeneric
microorganisms for bioremediation are still at the re-
search stage.
Obtaining Further Information

Persons who are unsure as to whether their microor-
ganisms would be subject to reporting under TSCA
section 5 should consult with EPA before preparing
any submission. The "Points to Consider" document,
the Federal Register notice describing EPA's  current
policy (51 FR 23313, June 26,1986), and the proposed
TSCA biotechnology rule (59 FR 45526, September 1,
1994) are  all available from EPA's TSCA Hotline at
202-554-1404. Additionally, as  part of an interagency
"streamlining" initiative, EPA made the proposed rule,
the "Points to Consider," and various other support
documents available electronically. They may be ac-
cessed through the Internet at gopher.epa.gov. EPA
plans to continue this approach and make the final rule
and key support documents available electronically.

For further  information about the proposed TSCA
biotechnology rules or about submitting a PMN for a
microorganism under EPA's current program, contact
Ellie Clark at 202 260-3402 or  David Giamporcaro at
202 260-6362.
                                                 10

-------
                                                                         Bioremediation in.the Field
               ORD Laboratories
               Restructured To
               Strengthen
               EPA's Research
               Focus
EPA's Office of Research and Development (ORD)
recently completed a  reorganization designed to
strengthen the Agency's ability to respond to the great-
est threats  to  environmental quality and human
health. ORD's 12 laboratories have been reorganized
into two national centers and three national laborato-
ries (see Figure 1). The new National Risk Manage-
                                             ment Research Laboratory (NRMRL) will take the lead.
                                             in bioremediation research. NRMRL will collaborate
                                             in this research with the National Health and Environ-
                                             mental  Effects  Research Laboratory's (NHEERL's)
                                             Gulf Ecology Division in Gulf Breeze, Florida; the
                                             Environmental Carcinogenesis Division in Research
                                             Triangle Park, North Carolina; and the National Expo-
                                             sure Research Laboratory's (NERL's) Ecosystems Re-
                                             search  Division in Athens, Georgia. At  NRMRL,
                                             bioremediation research will be conducted principally
                                             in the Land Remediation and Control Division in Cin-
                                             cinnati, Ohio, and bioremediation research on in situ
                                             biotreatment of ground water will be  conducted by
                                             NRMRL's Subsurface Protection and Remediation
                                             Division in Ada, Oklahoma.
                                             AA
                                        DAA/Science
                                         DAA/Mgmt.
                                            Staff
     Office of Resources
       Management &
        Administration
Office of Research &
      Science
    Integration
                                      Office of Science
                                            Policy
     Key
I   I Headquarters
    National Lab/Center
HI Joint Representation
Figure 1. Office of Research and Development Reorganization
                                             11

-------
Bioremediation in the Field
EPA Bioremediation Publications
To order EPA documents, call 513-569-7562 or write to
ORD Publications, U.S. Environmental Protection
Agency, 26 West Martin Luther King Drive, Cincinnati,
OH45268. To order NTIS documents, call 800-553-6847 or
write, to National Technical Information Service, 5285
Port Royal Road, Springfield, VA22161.
Bioremediation in the Field Search System
   (BFSS)	 EPA/540/R-95/508b
Bioremediation in the Field Search System
   (BFSS) User Documentation	EPA/R-95/508a
Assessing UST Corrective Action Technologies:
   Lessons Learned About In Situ Air Sparging
   at Denison Avenue—
   Cleveland, OH	 EPA/600/R-95/040
Natural Attenuation of Hexavalent Chromium
   in Ground Water and Soils		PB95-182614
Engineering Bulletin: In Situ Biodegradation
   •Treatment	EPA/540/S-94/502
Hydrocarbon Spill Screening Model
   (HSSM)—Volume 1: User's Guide . NTIS PB94-189487
Hydrocarbon Spill Screening Model
   (HSSM)—Volume 2: Theoretical
   Background and Source Codes	EPA/600/R-94/039b
Methods  for Monitoring Pump-and-Treat
   Performance	 EPA/600/R-94/123
Superfund Innovative Technology Evaluation
   Program—Technology Profiles—Seventh
   Edition	 EPA/540/R-94/526
Emerging Technology Summary: Pilot-Scale
   Demonstration of a Two-Stage
   Methanotrophic Bioreactor for
   Biodegradation of Trichloroethene in
   Ground Water	EPA/540/S-93/505
Engineering Issue: In Situ Bioremediation of
   Contaminated Unsaturated
   Subsurface Soils	EPA/540/S-93/501
Guide for Conducting Treatability Studies
   under  CERCLA: Biodegradation Remedy
   Selection—Interim Guidance	 EPA/540/R-93/519a
Guide for Conducting Treatability Studies
   under  CERCLA: Biodegradation Remedy
   Selection—Quick Reference
   FactSheet	 EPA/540/R-93/519b
Handbook for Constructed Wetlands
   Receiving Acid Mine Drainage .. NTIS PB93-233914AS
SITE Emerging Technologies: Bioscrubber for
   Removing Hazardous Organic Emissions
   from Soil, Water, and Air Decontamination
   Processes	 NTIS PB93-227205
Summary Paper: In Situ Bioremediation of
  Contaminated Vadose Zone Soil	EPA/540/S-93/502
Symposium on Bioremediation of Hazardous
  Wastes: Research, Development, and Field
  Applications	EPA/600/R-93/054
Bioremediation Case Study Collection: 1991
  Augmentation of the Alternative
  Treatment Technology Information Center
  (ATTIC)	EPA/600/R-92/043
A Study to Determine the Feasibility of Using
  a Ground Penetrating Radar	NTIS PB92-169382
Bioremediation of Hazardous Waste	EPA/600/R-92/126
Characterizing Heterogeneous
  Wastes	NTIS PB92-216894
Effect of Sodium Chloride on Transport of
  Bacteria in a Saturated Aquifer
  Material	NTIS PB92-110428
Fundamentals of Ground-Water
  Modeling	NTIS PB92-232354
Guide for Conducting Treatability Studies
  under CERCLA, Aerobic Biodegradation
  Remedy Screenings	NTIS PB92-109065
In Situ Bioremediation of Contaminated
  Ground Water	NTIS PB92-224336
Methodologies for Evaluating In Situ
  Bioremediation of Chlorinated
  Solvents	NTIS PB92-146943
Oil Spill Cleanup	NTIS PB92-110469
TCE Removal from Contaminated Soil and
  Ground Water	NTIS PB92-224104
Technology Evaluation Report: Biological
  Treatment of Wood Preserving Site Ground
  Water by Biotrol, Inc	NTIS PB92-110048
Alternative Biological Treatment Processes for
  Remediation of Creosote-Contaminated
  Materials: Bench-Scale Treatability
  Studies	NTIS PB91-179085
Anaerobic In Situ Treatment of Chlorinated
  Ethenes	NTIS PB91-137067
Applications Analysis Report: Biological
  Treatment of Wood Preserving Site Ground
  Water by Biotrol, Inc	NTIS PB91-227983
Approach to Bioremediation of Contaminated
  Soil	NTIS PB91-116152
Movement of Bacteria through Soil and
  Aquifer Sand	NTIS PB91-164277
Nitrate for Biorestoration of an Aquifer
  Contaminated with Jet Fuel	NTIS PB91-164285
                                                    12

-------
                                                                                  Bioremediation in the Field
EPA Bioremediation Publications (continued)
Protocol for Testing Bioremediation
  Products against Weathered Alaskan
  Crude Oil	  NTIS PB91-137018

Reductive Dehalogenation: A Subsurface
  Bioremediation Process	  NTIS PB91-144873

Selection of Nutrients to Enhance
  Biodegradation for the Remediation of Oil
  Spilled on Beaches	;	  NTIS PB91-233304

Total Organic Carbon Determinations in
  Natural and Contaminated Aquifer
  Materials	  NTIS PB91-129205

Understanding Bioremediation: A Guidebook
  for Citizens	EPA/540/2-91/002

Action of a Fluoranthene-Utilizing Bacterial
  Community on Polycyclic Aromatic
  Hydrocarbon Components
  of Creosote	  NTIS PB90-245721

Alaskan Oil Spill Bioremediation
  Project	  NTIS PB.90-216466

Assessing Detoxification and Degradation of
  Wood Preserving and Petroleum Wastes in
  Contaminated Soil	  NTIS PB90-245275

Available Models for Estimating Emissions
  Resulting from Bioremediation Processes: A
  Review	  NTIS PB90-228610

Bioremediation of Contaminated
  Surface Soil	  NTIS PB90-164047

Creosote-Contaminated Sites	  NTIS PB90-129552

Enhanced Bioremediation Utilizing Hydrogen
  Peroxide as a Supplemental Source of
  Oxygen: A Laboratory and
  Field Study	  NTIS PB90-183435
Laboratory Studies Evaluating the Enhanced
  Biodegradation of Weathered Crude Oil
  Components through the Application of
  Nutrients	NTIS PB90-264011
Role of Microorganisms in the Bioremediation
  of the Oil Spill in Prince William Sound,
  Alaska	NTIS PB90-263070
Sequential Reductive Dehalogenation of
  Chloranilines by Microorganisms from a
  Methanogenic Aquifer	NTIS PB90-117219
Comparison of Methods to Determine Oxygen
  Demand for Bioremediation of a
  Fuel-Contaminated Aquifer.	NTIS PB89-207351
In Situ Bioremediation of Spills from
  Underground Storage Tanks: New
  Approaches for Site Characterization,
  Project Design, and Evaluation of
  Performance	NTIS PB89-219976
Microbial Removal of Halogenated Methanes,
  Ethanes, and Ethylenes in an Aerobic
  Soil Exposed to Methane
  Gournal Version)	NTIS PB89-103196
Field Evaluation of In Situ Biodegradation for
  Aquifer Restoration	NTIS PB88-130257
Interactive Simulation of the Fate of
  Hazardous Chemicals during
  Land Treatment of Oily Wastes: Ritz
  User's Guide	NTIS PB88-195540
Removal of Volatile Aliphatic Hydrocarbons in
  a Soil Bioreactor	NTIS PB88-180393
Transformation of Halogenated Aliphatic
  Compounds	NTIS PB88-170568
Microbial Decomposition of Chlorinated
  Aromatic Compounds	EPA/600/2-86/090
                                                    13

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          FIELD APPLICATIONS OF BIOREMEDIATION3
REG
I
I
I
I
I
-*•
I
3c
f ,
I
I -
>
SITE/
LOCATION/
LEAD
Gaird and McGuire
Holbrook, MA
CERCLA fund lead
Charlestown Navy Yard
Boston, MA
CERCLA slate lead
Coakley Landfill
North Hampton, NH
CERCLA enforcement lead
Genera! Electric
Pittsfield, MA
RCRA lead (federal)
General Electric— Woods Pood
"Phtsfleld, MA
RCRA lead (federal)
Hanseom AfrFcSrcft Base1*
BedftmtMA ', \ '
Federal facility < -. "
Building H24 s -,
Cfitniding 1813 • ~~ ' : *
Buil<3ingU83Q
Building 1843 " " *, "\
Iron Horse Park, Operable
Unit 1 (B&M Lagoon)
Billerica, MA
CERCLA enforcement lead
Fease, Air Force Base* " ^
Pease AJ?B KJrt
Federal facility
CONTACT/
PHONE
NUMBER
David Lederer
(617)573-5738
Federal organization
Stephen Carlson
(617)242-5680
State organization
Steve Calder
(617)573-9626
Federal organization
JoanBJake
(202)260-6236
Federal organization
Joan Blake
(202)260-6236
-Federal organization
Jerry HansetL " ^ *
<210).536-4331 - sV
Federsi'organizattoit
\,
; ^ ^ ^ >> s>
v •* "^
> v% v>^s
Don McElroy
(617)223-5571
Federal organization
LtyToiSos^Mtlfcr
^2ib)5M43$jT -
Rsdefal organizattoB
MEDIA/
CONTAMINANT
Ground water (BTEX; chlordanc; creosote)
Basis: MCLs
Other contaminants: arsenic, lead
Volume: 300 gallons per minute
% of total vol. at site: 100%
Sediments (creosote; PAHs)
Ground water (ammonia)
Basis: NPDES requirements
Sediments (PCBs, 2 pgm)
Basis; performance-based"
Voiurne; 12 cubic meters
„% of tots! vol. at site: less than 1% •
Sediments (PCBs, 1 ppm)
Basts: performance-based N - > ' ^ "
Volume:. 250 gallons
%ttf total vol. at site: iess than 1% \ \. • '
Sot!(ben^n«;^hy]b«Wne;toluet»,TR?Hsixyterie) \ ^
Basts' state regulations '-T \ „ s "N s " , v
, t \ N ( x
Spii^TBX;TiTOsJ\; ":-'\vV" ' -' ,% "- . ^
" <- "x -^ ^ o x'Cc -- ,, x"s
" ^.\\\ , sV ,\! .•> -i; ,l,yx S0"^ -v ^ .
•Soil (benzene; efb.yttfeHiZeBSUoteeae;s'FRPHs;xyteHe)^ s
J ^4< ^ N « x
Soil Benzene; ethytbeS^BeitSJ^ettS; JRPHs; xytefteK , " ^
^s >^OV" ^s ^
Sludge (PAHs, 1 ppm; TPHs, 5,000 ppm)
Vadose soil (PAHs, 1 mg/kg; TPHs, 5,000 mg/kg)
Basis: risk-based
Other contaminants: lead
Volume: 20,000 cubic yards
% of total vol. at site: 100%
"Soil (benzsnetfilfeyJbenzBHe; toluene; TjRPHs; xyfeae)
<- , ' x , •> ^, s
STATUS
Full-scale remediation has been underway since
01/93.
Full-scale bioremediation is not planned.
Laboratory-scale studies were completed 09/93.
Full-scale remediation is planned. Currently in
predesign. Expected start 01/96.
FulJ-scate bioremediation is not planned
Laboratory-scale and pilot-scale studies are being ,
conducted.
Full-scale KoremeSiatiori is not planned.
Laboratory-scale and pilot-scale studies are being
conducted. „ - '
FulJ-scaleremedi9lionisj>toiied< Pilot-scale ^ -
studies were completed 04/94, Started 04/93 ^_ " -
^ j " » ' "I *. \> ,
S" % C S''-° ^ "• V ^ - ""X X "-X.
FaH-scajeremediatojs^lanried. BloHcal6s ^x ^
studies. were compteted:fl4/94. Staffed 0^^^^"- °
C- X XV>H \v 0 \.^,» '_ \
Fall-seafe remedt^koa is|toined. Pilot-scale ^ • \ x<
swdwswer«<;or •
%°SC^S V\'N ~ N
Full-scale remedtaawi%ptotiea.JPiloHfia«,x"
stadias were cbtapfetel I i/94. Started JOS^v
, \ " .,"'; s-V '.^y'""^ <• ;
Full-scale remediation has been underway since
05/92. Laboratory-scale studies were completed
01/88.
Pilot-scale studies were completed 09/93.
Full-sca1ereffiediatioaJS"^ttned,P^IoWiate^
x •> v N - , x^ ^ *
\ <
TREATMENT
Ex situ treatment, activated sludge, completely mixed flow.
Aerobic conditions, exogenous organisms.
In situ treatment, sediment bioremediation. Oxygen source,
nutrient addition. Aerobic conditions, indigenous organisms.
Bioremediation treatment not yet established.
Ex situ treatment, sequencing batch reactor, batch flow.
Anaerobic conditions, indigenous organisms
In situ treatment, confined ireatrrient facility for sediments,
Nutrient addition Anaerobic conditions, indigenous^
organisftjs.' % ' t -" > ., " '
In situ treatment, biovestlrig, Aej'obic conditions, indigenous
organisms. \ - ^s »x
Inisit«|restnient bioventtng, Ae8>rJfeco,ndiaons^indigenoasX.-
oiganisias* •.* ^ , ,y^ " ^ " > * " Xs
Ss ^ ^ V N- ^ ^ "^ V J
- to silu treatment, bioventiag. Aerobic conditions, indigenotis >
^organisms. t ^" v \ ^ 1%^""^." -f
.Jn tea treatment, |loventfsg. Aerobic condi^on^, ui^i^nq^t ^_
organisms/- ° "" ' - * ^ -s . " \MN x^ > "s
Ex situ land treatment. Aerobic conditions, indigenous
organisms.
& site treatment* bio^eafitig- A^ffltac conditions-, mdigen&as «
-' * ' ^ <
2 CERCLA/RCRA/UST sites considering, planning, operating, or having used bioremediation.
b Indicates a new site.

-------
                                                                                                                                                           (cont.)
 REG
    SITE/
LOCATION/
   LEAD
CONTACT/
  PHONE
 NUMBER
     MEDIA/
CONTAMINANT
                                                                                                                     STATUS
                 TREATMENT
       Sylvester
       Nashua, NH
       CERCLA state lead
       Process 2
       Sludge reactor w/aeration
                     Chet Janowski
                     (617)573-9623
                     Federal organization
                 Ground water (benzene, 340 ppb; chloroform, 1,505 ppb;
                 MEK, 8,000 ppb; 1,1,2-TCA, 1.7 ppb; chlorobenzene,
                 llOppb; 1,1-DCA, 1.5ppb; l,l,l-TCA,200ppb;methylene
                 chloride, 12.3K ppb; methylmethacrylate, 350 ppb; PCE,
                 57 ppb; phenols, 400 ppb; TCE, 1,500 ppb; toluene,
                 2,900 ppb; trans-1,2-DCE, 1,800 ppb; vinyl chloride, 95 ppb)
                 Basis: New Hampshire Drinking Water Standards
                 Other contaminants: selenium

                 Ground water (benzene, 340 ppb; chloroform, 1,505 ppb;
                 MEK, 8,000 ppb; 1,1,2-TCA, 1.7 ppb; chlorobenzene,
                 llOppb; 1,1-DCA, 1.5ppb; l,l,l-TCA,200ppb;methylene
                 chloride, 12.3K ppb; methylmethacrylate, 350 ppb; PCE,
                 57 ppb; phenols, 400 ppb; TCE, 1,500 ppb; toluene,
                 2,900 ppb; trans-1,2-DCE, 1,800 ppb; vinyl chloride, 95 ppb)
                 Basis: New Hampshire Drinking Water Standards
                 Other contaminants: selenium
                                                                                                     Predesign.
                                                                                                                                                Ex situ treatment, extended aeration.
                                                                                         Full-scale remediation has been underway since
                                                                                         01/86. Pilot-scale studies were completed 01/83.
                                                                                                                                                Ex situ treatment, activated sludge.
                                 ii»Wi:i
                                 ^^Tjraf'or|S^attftti
       American Linen
       Stillwater, NY
       CERCLA state lead
                     Frank Peduto
                     (518)457-9412
                     State organization
                 Soil (BTEX lube oil; PAHs; VOCs)
                 Basis: TCLP
                 Volume: 4,375 cubic yards
                 % of total vol. at site: 100%
                                   Full-scale remediation was completed 08/92.
                                   Started 07/91.
Ex situ land treatment. Aerobic conditions, indigenous
organisms.
b Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

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                    FIELD APPLICATIONS  OF BIOREMEDIATION (cont.)
 REG
                   SITE/
                LOCATION/
                   LEAD
CONTACT/
  PHONE
 NUMBER
     MEDIA/
CONTAMINANT
                                                                                                                 STATUS
                TREATMENT
       Dayco Corporalion-L.E.
       Carpenter Company11
       Wharton, NI
       CERCLA slate lead
                                    Christina Purcell
                                    (609)633-1455
                                    State organization
                Vadosesoil (bis(2-ethylhejtyl)phtbalate, 100 mg/kg; xylene,
                lOmg/kg)
                Ground water (bis(2-ethylhexyl)phthalate, 30 \ig/L; xylene,
                20ug/L)
                Basis (vadose soil): slate standard
                Basis (ground water): state standard forreinjection
                Other contaminants: lead, antimony, PCBs
                Volume: 31,500 cubic yards
                % of total vol. at site: 97%
                                 Full-scale remediation is planned. Currently in
                                 predesign. Laboratory-scale studies were
                                 completed 05/92. Started 1 1/91.
                                 Pilot-scale studies are planned.
In situ treatment, ground-water bioremediation, soil
bioremediation (in situ land treatment). Ex situ treatment, type
of reactor (for ground water) will be determined in the
remedial design. Hydrogen peroxide, oxygen source, nutrient
addition (ammonium and phosphate). Aerobic conditions,
indigenous organisms.
       DupontPoropion Lakes'1
       PomptoB Lakes, NJ
       JRCRAlwd (state)
                                    MrfceSwindoU
                                    (201)492-1739
                                    Industry
                Ground water {1,1-DCA, 70 ppb; l,J,t-TCA, 30 ppb;
                1,1-dichloroethylene, 2 ppb, 1,2-DCA, 2 ppbi cis-l,2-DCE,
                10ppE>;PCE, 1 ppb.TCE, 1 ppb;trans-l,2-DCE, tOOppt);
                vinyl chloride, 5 ppb)
                Other contaminants: metal (ihercury, iead, and selenium)
                contaminaa'bft ispresent in other portions of me site bpt is
                not associated with the solvent contoinatiQtt
                Voltane- 56,OOOxmWe feet
                % of tola! vot atsite: 0.01%
                                 Full-scale remediation Js pferoed. Currently in
                                 predesign. Laboratory-scale studies are planned.
                                 Pilot-scale stadiesliave been underway since
                                 12/93.
lit situ treatment, ground-water bloternediaflori Nutrient
addition. Anaerobic conditions,
ON
       FAA Technical Center™ '
       AreaD   ^  x
       AslanbeCownty.JSiJ
                                   Bs^y Donovan
                Ground waterXfaetizene, I gpb,  thylbenzsne, 5 ppb; acjtone;
                                                  S-chteropheaol,^ pp6, aceaapnthene^ 10 ppb; HWJiyfene
                                                  ehrqndfe, 2pr^;oarAltaleae?o-?je^0tJ,^enaiitlir«a«, 10pp
                                                  jifeeaol, 10 ppb, pyrais, 20 ppb; toluene, 5 prib; xytenej
                                                                    x                       "
                                 Ftrfl-sealfc remediation i$ planned. Cluienaji being
                                 installed. Laboratory-scale snjttues'weie completed •
                                 H/90. Started IWfO.
                                       '
       Fireman's Training Center1"
       OldBethpage,NY
       CERCLA state lead
                                    Peter J.Witkowski
                                    (516)571-9600
                                    Municipal
                                    organization
                Ground water (benzene, 0.7 ppb; BTEX, 50 ppb;
                ethylbenzene, 5 ppb; toluene, 5 ppb; xylene, 5 ppb)
                Basis: state ground-water regulations
                Other contaminants: low levels of high molecular weight
                PAHs and other petroleum product related semi-volatile '
                organics
                                 Full-scale remediation is planned. Currently in
                                 design. Expected start 05/97. Pilot-scale studies
                                 were completed 04/94. Started 01/94.
Ex situ treatment, fluidized bed, completely mixed flow.
Aerobic conditions, exogenous organisms.
       General Electric —
       Hudson River, NY
       CERCLA enforcement lead
                                   Jim Harrington
                                   (518)485-8792
                                   State organization
                Sediments (PCBs)
                Other contaminants: cadmium, chromium, lead
                Volume: 150 cubic feet
                % of total vol. at site: less than 1%
                                 Full-scale bioremediadon is not planned.
                                 Laboratory-scale studies have been completed.
                                 Pilot-scale studies were completed 01/92.
In situ treatment, confined treatment facility for sediments.
Aerobic conditions, indigenous organisms.
                                                        &
       General Motors — Central
       Foundry Division
       Massena, NY
       CERCLA enforcement lead
                                    Lisa Carson
                                    (212) 264-6857
                                    Federal organization
                Sediments (PCBs, 1 ppm)
                Sludge (PCBs, 10 ppm)
                Soil (PCBs, 10 mg/kg)
                Basis: risk-based
                Volume: 350,000 cubic yards
                                 Full-scale bioremediation is not planned.
                                 Laboratory-scale studies were completed 12/93.
                                 Started 04/93.
Ex situ treatment, slurry reactor, batch flow. Aerobic
conditions, indigenous organisms.
                                                                                                                                                                                                     a'
                                                                                                                                                                                                     I
b Indicates a new site.
c Indicates that the site has been updated.

-------
                  FIELD APPLICATIONS  OF BIOREMEDIATION (cont.)
 REG
        SITE/
     LOCATION/
        LEAD
  CONTACT/
    PHONE
   NUMBER
    MEDIA/
CONTAMINANT
                                                                                                    STATUS
                                                                                            TREATMENT
       Indiana Wood Treating
       Bloomington, IN
       CERCLA fund lead
       Process 1
       Process 2
                       Steve Faryan
                       (312)353-9351
                       Federal organization
                Soil (PAHs, 100 mg/kg; total PAHs, 500 mg/kg)
                Basis: removal risk-based
                Volume: 22,000 tons
                % of total vol. at site: 100%
                                             Water (total PAHs)
                             Full-scale remediation was completed 08/94.
                             Started 11/92. Laboratory-scale studies were
                             completed 06/92. Started 04/92.
                             Pilot-scale studies were completed 07/92. Started
                             06/92.

                             Predesign.
                                                                              Ex situ treatment, pile. Aerobic conditions, indigenous
                                                                              organisms.
                                                                                                                          In situ treatment, ground-water bioremediatioh. Hydrogen
                                                                                                                          peroxide. Aerobic conditions, indigenous organisms.
                             Matt-
                                                                                                                            situ land treatment. Aerobic conditions, exogenous and

Mobil Terminal
Buffalo, NY
CERCLA enforcement lead
Robert Leary
(716)851-7220
Federal organization
Soil (diesel; gasoline)
Basis: NYDEC guidance values based on TCLP
Other contaminants: lead
Volume: 15,000 cubic yards
% of total vol. at site: 100%
                             Full-scale remediation has been underway since
                             07/91. Laboratory-scale and pilot-scale studies
                             have been completed.
       Nascolite
       Millville, NJ
       CERCLA enforcement lead
                      Farnaz Saghafi
                      (212)264-4665
                      Federal organization
               Ground water (methylmethacrylate organics, 560 ppb)
               Basis: risk-based
               Other contaminants: lead
                             Full-scale remediation is planned. Currently in
                             design. Laboratory-scale studies were completed
                             06/92, Started 04/92.
                             Pilot-scale studies were completed 06/94. Started
                             02/93.
                                                                              Ex situ treatment, fluidized bed, plug flow. Aerobic
                                                                              conditions, indigenous organisms.
b Indicates a new site.            : ,
c Indicates that the site has been updated.;
Shading indicates a non-CERCLA site.

-------
                      FIELD  APPLICATIONS  OF BIOREMEDIATION  (cont)
    REG
    SITE/
LOCATION/
   LEAD
CONTACT/
  PHONE
 NUMBER
    MEDIA/
CONTAMINANT
STATUS
TREATMENT
          Nassau County Fire Training
          Area"
          Belhpage, NY
          CERCLA state lead
          Bioventing
          Fluidized bed
                    George Heilzman
                    (518)457-1641
                    Slate organization
                Vadose soil (semivolariles, 500 mg/kg; benzene, 0.6 mg/kg;
                elhylbenzene, 5.5 mg/kg; toluene, 1.5 mg/kg; TPHs; xylene,
                1.2 mg/kg)
                Basis: health-based
                Volume: 20,000 cubic yards
                % of total vol. at site: 54%

                Ground water (benzene, 0.7 ppb; elhylbenzene, 5 ppb;
                acetone, 50 ppb; 2-methylnaphthalene, 10 ppb; MEK,
                50 ppb; naphthalene, 10 ppb; toluene, 5 ppb; xylene, 5 ppb)
                Basis: ground-water effluent standards
                Other contaminants: chlorinated solvents and degradation
                products (TCE, PCE, TCA, DCE, DCA, VC) and inorganics
                (Fe, Mn, Pb, Cr, Ba)
                Volume: 400 gallons per minute
                % of total vol. at site: 25%
                                 Full-scale bioremediation is not planned.
                                 Pilot-scale studies were completed 05/94. Started
                                 02/94.
                                                                                     Full-scale remediation is planned. Currently in
                                                                                     design. Expected start 09/95. Pilot-scale studies
                                                                                     were completed 05/94. Started 02/94.
                         In situ treatment, bioventing. Nutrient addition (ammonium
                         phosphate). Aerobic conditions, indigenous organisms.
                                                                         Ex situ treatment, fluidized bed, completely mixed flow.
                                                                         Aerobic conditions, exogenous organisms.
00
          Naval Air Engineering Station
          Lakehurst, NJ
          CERCLA enforcement lead
          Bioventing
          Passive bioremediation
                    Joe Reiner
                    (908)323-7800
                    Federal organization
                Vadose soil (butylmethylketone; ethylbenzene, 100 mg/kg;
                2-methylnaphthalene; fluorene, 100 mg/kg; naphthalene,
                100 mg/kg; phenanthrene; toluene, 500 mg/kg; TPHs,
                10K mg/kg; xylene, 10 mg/kg)
                Volume: 3,000 cubic yards
                % of total vol. at site: 100%

                Ground water (TCE, 5; 1,1,1-TCA,200; 1,1-DCA; 1,1-DCE,
                7; 1,2-DCE, 70; chloroform, 100; cis-l,2-DCE, 70;
                tetrachloroethene, 5; vinyl chloride, 2)
                                 Full-scale remediation is planned. Currently in
                                 design. Pilot-scale studies were completed 06/94.
                                 Started 05/94.
                                                                                     Full-scale remediation is being conducted.
                          In sini treatment, bioventing. Oxygen source. Aerobic
                          conditions, indigenous organisms.
                                                                         Aerobic conditions, indigenous organisms.
          Niagara Mohawk Power
          Corporation
          Saratoga Springs, NY
          CERCLA enforcement lead
          Process I

          Process 2
                    Edward Neuhauser
                    (315)428-3355
                    Industry
                Soil (PAHs)
                Basis: potential for leaching to ground water
                                                     Ground water (PAHs)
                                                     Basis: potential for leaching to ground water
                                 Laboratory-scale studies were completed 05/92.
                                 Started 02/92.
                                                                                     Predesign.
                          In situ treatment, soil bioremediation (in situ land treatment).
                          Nutrient addition (type not specified). Aerobic conditions,
                          indigenous organisms.
                                                                                                       Ex situ treatment, fluidized bed. Aerobic conditions,
                                                                                                       indigenous organisms.
          Novak Farmb
          Chenango County, NY
          CERCLA state lead
                    Gary E. Kline
                    (518)457-5636
                    State organization
                Soil (SVOCs, 50 mg/kg; xylene, 1.2 mg/kg)
                Basis: risk-based from ground-water standards partitioning
                Volume (vadose soil): 10k cubic yards
                Volume (saturated soil): 25k cubic yards
                                 Full-scale remediation is planned. Currently in
                                 design. Expected start 04/96, expected completion
                                 10/99. Laboratory-scale studies were completed
                                 12/93.
                          Ex situ treatment, pile, bioventing of off-gases. Aerobic
                          conditions, indigenous organisms.
          Osmose
          Buffalo, NY   •
          CERCLA state lead
                    Jim Harrington
                    (518)485-8792
                    State organization
                Vadose and saturated soil (BAP, 10 mg/kg; PAHs, 50 mg/kg)
                Soil (BTEX fuel oil; creosote, 473 mg/kg)
                Basis: risk-based
                Volume: 670 cubic yards
                % of total vol. at site: 30%
                                 Full-scale remediation has been underway since
                                 09/90.
                          Ex situ land treatment. Aerobic conditions, indigenous
                          organisms.
                                                                                                                                                                                                    a'
    Indicates a new site.
   c Indicates that the site has been updated.
                                                                                                                                                                                                    1

-------
                  FIELD APPLICATIONS OF  BIOREMEDIATION  (cont.)
 REG
   SITE/
LOCATION/
   LEAD
CONTACT/
  PHONE
 NUMBER
    MEDIA/
CONTAMINANT
STATUS
TREATMENT
       Sweden 3 Chapman
       Town of Sweden, NY
       CERCLA state lead
       Biovault

       Bioventing
       Combined process
                  Dr. Nick Kolak
                  (518)485-8792
                  State organization
              Vadose soil (chlorinated hydrocarbons; hydrocarbons)
              Basis: risk-based
              Volume: 200 cubic yards
              % of total vol. at site: 1.5%

              Vadose soil (TCE; 1,2-DCE, 7.35 ng/kg; acetone, 3.3 ng/kg;
              MEK, 6.35 u.g/kg; toluene, 45 (ig/kg; vinyl chloride,
              3.42 ng/kg)
              Volume: 1,000 cubic yards
              % of total vol. at site: 7.7%

              Soil (acetone, 0.2 mg/kg; 2-butanone, 0.6 mg/kg;
              4-methy!-2-pentanone, 2 mg/kg; 1,1,1-TCA; cis-l,2-DCE;
              TCE, 1.5 mg/kg; tetrachloroethene, 2.5 mg/kg; toluene; vinyl
              chloride; xylene)
              Ground water (acetone; 2-butanone; 4-methyl-2-pentanone;
              1,1,1-TCA; cis-1,2-DCE; TCE; tetrachloroethene; toluene;
              vinyl chloride; xylene)
              Volume (vadose soil): 75 cubic yards
              Volume (saturated soil): Ik cubic yards
                               Full-scale remediation is planned. Pilot-scale
                               studies are being conducted.
                                                                              Full-scale remediation is planned. Currently in
                                                                              predesign. Laboratory-scale studies were
                                                                              completed 12/94. Started 08/94.
                                                                              Pilot-scale studies were completed 12/94. Started
                                                                              07/94.

                                                                              Full-scale remediation is planned. Currently in
                                                                              predesign. Pilot-scale studies are being conducted.
                       Ex situ treatment, biovault (non-composting enclosure), batch
                       flow. Aerobic and anaerobic conditions, indigenous
                       organisms.
                                                                    Oxygen source, nutrient addition (anhydrous ammonia and
                                                                    methane gas). Aerobic conditions, indigenous organisms.
                                                                    In situ treatment, air sparging. Ex situ treatment, fixed film.
                                                                    Nutrient addition (type not specified), batch flow. Aerobic
                                                                    conditions, exogenous organisms.
  Ill
       Avtex Fibers
       Front Royal, VA
       CERCLA enforcement lead
                  Bonnie Gross
                  (215) 597-0491
                  Federal organization
              Ground water (carbon disulfide)
              Other contaminants: arsenic, zinc, lead, cadmium, carbon
              disulfide, hydrosulfide
              Volume:  1,000,000 gallons per day
                              Full-scale remediation is being conducted.
                       Ex situ treatment, activated sludge, completely mixed flow.
                       Aerobic conditions, indigenous organisms.
11 Indicates a new site.     ,   :
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

-------
                     FIELD APPLICATIONS  OF  BIOREMEDIATION  (cont.)
    REG
        SITE/
     LOCATION/
        LEAD
CONTACT/
  PHONE
 NUMBER
    MEDIA/
CONTAMINANT
                                                                                                            STATUS
               TREATMENT
     Hi
Boiling Air Force Base1*
Washington, DC
Federal facility
Building 18

Former UST Farm
                                 Jerry Hansen
                                 (210)536-4331
                                 Federal organization
               Soil (benzene; ethylbenzene; toluene: TRPHs: xylene)
                                                  Soil(avgas;BTEX)
                               Full-scale remediation is planned. Pilot-scale
                               studies were completed 09/94. Started 05/93.
                                                           Full-scale remediation is planned. Pilot-scale
                                                           studies were completed 02/94. Started 1 1 193.
In situ treatment, bioventing. Aerobic conditions, indigenous
organisms.
                                                                     ID situ treatment, bioventing. Aerobic conditions,:in
                                                  Grojtnd water (solvents)  "    > •  • '
                                                  Other contaminants;' Iron, inanganese,,  v
                                                                                                    .        .
                                                                                             Ptio^scale Sadies liave be^ns«ndenvay
                                                                                                                           tn situ treatmen^air'spargiag.
                                                                                                                                             s
                                                                 r):
                                         Vadose soil      \,   s T,   ' yX
                                         Volume; &&OQflcujMcyanisv - "    "  - v " "- ,
                                         % of total voi. at lite: 90%    _
                                                                                                  In siw treatraentt bioventing, B^sira land treatment. Aerobic-
                                                                                                  conditions, ittdtgenoa'Torgantsms.        ^-s >    NV  -
                                                                                                                                                                                          i
                                                                                                                                                                                          &/
                                                                                                                                                                                          a
   b Indicates a new site.
   c Indicates that the site has been updated.

-------
               FIELD  APPLICATIONS OF BIOREMEDIATION  (cont.)
REG
       SITE/
    LOCATION/
       LEAD
 CONTACT/
   PHONE
  NUMBER
              MEDIA/
           CONTAMINANT
                                                                                       STATUS
                                                                                                                        TREATMENT
||
 III
Greenwood Chemical
Newtown, VA
CERCLA fund lead
Philip Rotstein
(215)596-9023
Federal organization
Vadose soil (toluene; acetone; chlorobenzene; naphthalene;
VOCs)
Basis: treatability study in progress
Other contaminants: heavy metals and semi-volatile
organics-SVOC (TICs) are present.
Volume; 500 cubic yards
Full-scale remediation is planned. Laboratory-scale
studies have been completed. "
Pilot-scale studies have been underway since
09/93.
In situ treatment, confined treatment facility for sediments.
Aerobic conditions, indigenous organisms.
 III
     L.A. Clarke & Son
     Fredericksburg, VA
     CERCLA enforcement lead
                    Andrew Palestini
                    (215)597-1286
                    Federal organization
              Sediments
              Soil (creosote)
              Volume: 119,000 cubic yards
              % of total vol. at site: 25%
                                     Full-scale remediation is planned. Currently in
                                     design. Pilot-scale studies have been underway
                                     since 07/92.
                                In situ treatment, creosote recoveiy, landfarming. Aerobic
                                conditions.
                              organi;
  lit
                         Brands
     Process 1\ >»*-, !    «  '


     ProcessgO ^ •"<; "-    '<• ^
      *,*"*** rC ,*    f  "^\. J.^
b Indicates a new site.      .     :
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.
                                                                                                                                                        f
                                                                                                                                                        I

-------
        FIELD APPLICATIONS OF BIOREMEDIATION (cont.)


REG
HI


111







m


UT'
-
Ui,


^ " •

^
-m


<
,
^



SITE/

LOCATION/
LEAD
Ordnance Works
Disposal Area
Morgantown, WV
CERCLA enforcement lead
SB Loveland Company, Inc.'
Philadelphia, PA
UST lead (state)
In situ ground-water :
bioreroediation
In situ soil




Standard Chlorine
New Castle, DE
CERCLA enforcement lead


Texas-Eastern Gas Pipeline - ,,
PA x ., x "Y°
Armaagh, PA * -
TSCAleadftetieral) \ <

X3.S."Navy SFCC, Site*3 Bum
"Pits* N"
Mechaaicsburg, PA x
jF^rai facility:;
' ^ . \ - " -" • x\
\ v ' ' ^ - '
x >-  '* x -
•Wheelwg-Pittsburih Steel
AUengort" '
Altenport, PA
PA Department oil < ,
EnvjroBfneiaal Resources
ojoviESnfing
Pile treatment
<
"•'">; -
_% s

CONTACT/

PHONE
NUMBER
Melissa Whittington
(215)597-1286
Federal organization

Gary Brown
(610)265-1510
Contractor/
engineering firm






Kate Lose
(215) 597-0910
Federal organization


Joan Blake ^
(202) 260-6236 _
Federal organization
f ;
LtAlanHeiHW
<7.17} 730-2129-
Federal organization^
,jlv
>S ^" " * \
XI X - '
'' v ' - v .
Patrick J, Smith
,{412)326-0275 5
Industry ^ ' <
v Xx - ' *
" '
•X
icyaTds ' - ^x - ^ ^'" ;
v^ C ' ^ X *> ' t ^ "N X ^
Vadose soil (TPHs, 2G0cmg/kg;aceh>Re,X6u,g$cgj ^ ^ ;
^chtorobenzene, 1 ftg/kg! raethylens chMide>0.84 tlg^cg; ~> *••

chloride. 1 16 mJk • VOfc^ !(»& ik V ^ ?V * * --J - " x
Other contamtnantsf PCBs, pesticides "metals «»CBl)«: yards „ ' x ^'N _^ - ^ • \ ;
^ x ^ - ^>!x °- -" !"vx " N>-, x " -> *o: , ' '"-
v - X ' " * X, ' '/x ' \ ^ , " '^
, " v >X- x '>s %x \- '' - /
Soil (TPHs; 5N00 mg/kg) 5 - < "4,,-. %>:: "' '\
Basis:-|JSk-ba$edVpADER gtiidarice dopiaaeot) ^ N* ' ^ s
Volutae: l.SOOcuWcyards^ , ^ *^'x 'v ,„;,."; "v \
«V total vol atsite: 15% '" - > ^ ^ ^' '" X < '



STATUS
Full-scale remediation is planned. Laboratory-scale
studies have been underway since 02/93.
Pilot-scale studies are planned.

PuJI-scaJe remediation was completed 09/94.


FuU-scaleieroediation was completed 09/94.



,- n ^ '
Full-scale bioremediation is not planned.
Laboratory-scale studies were completed 09/93.
Started 04/93.
Pilot-scale studies are planned.

JRjll-s»afereraediRti' v.^'!^^ \s's "," "^ ^ • :
s ' X* " ^ ^ ° " \>\' X V 1 ' ^^
N, '\ '^' ''''Yx^ NX ;\* C
J^ N ,x^ ."-. "J ^ Ixxt> ,% , ^,x ; U
il\ici^;'v" ;rl:;xx:>i
J^iil-^ale leitteoia^oltis pl^uned. Curceu^iy jrt x x>
pretiesiga* Laboratory-s^ale studte^have been * \x
uoyerway since 03/94 » „ ;\ ^ ,
4 ,x x x X\^'\,v • " - -'f '1\
r N ^;>; :>\1 " x"-xx x K?
< ' ' 5> ,^'s ', X"V4 S"""
X XN 1 < > *\x N> >S>> 'X '/N Vxx""
FulPscateieroedia(ionisp1aat!e4'Curreatly in .-t
desWnxxL&fe&a&fv-&%fe^!«die<-H^!&xcAtnAlfitefi = ;
05/94. StMes}04?9^ "x >*n> " "NX,, '' ?
,< x, ; 1 ;, f»~ %N x % ^\ > *^ x " '



TREATMENT
Ex situ land treatment. Aerobic conditions, indigenous
organisms.


la situ treatment, ground-water bioremediation. Nutrient
addition (not specified). Aerobic conditions, exogenous


In situ treatment, soil bioremediation {in situ land treatment).
Nutrient addition (not specified), Aerobic conditions,
exogenous organisms, j
1


In situ treatment, sediment bioremediation, soil
bioremediation (in situ land treatment). Ex situ treatment,
slurry reactor, to be determined. Nutrient addition (to be
determined). Aerobic and anaerobic conditions, indigenous
organisms.
Jit situ treatment, soif.ttioiemediation <3n situ land treatment)
"g* siftt foi
X^ < N s ? ^ ^ „ *^ s <*XN
, XN -X ,^ ., <
Ex situ featn»at,i!ile^comtiiBariori of treatmeaf pile with ^ ",
conditions/exogeiiotts and Jn v vv(
>S: v< s "x ' S ^1 * ° " ^ 5
- . x " \*x ; x- " ,,"• ^ f ^ N x\ ,>;V
!:^:Vv::v^J:"<:5:/:
In situ tsbarnK;tit,J)ioventi9glOxygen source. Aerobic * '•. '^
*'-- >^ " " ' v' " ! x •:'" s "v ' « 'S
\ x " ^ - X '"~" ' •"*"«,' '^'^ *
'x"X" ' ' -f ' -* , " S'X ' X^' -s '
& sifuffeatment, pie, Aerobic conditions, ifldi^nous ' % x

' SN"'S- " xx ' " , ' , ' ^ ^
\ .' • , 'xs, t S > ,_'',' ^ ^ J. ^

" Indicates a new site.
0 Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

-------
               FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
   SITE/
LOCATION/
   LEAD
CONTACT/
 PHONE
 NUMBER
   MEDIA/
CONTAMINANT
                                                                                     STATUS
            TREATMENT
  Hi
     Whitmoyer Labs
     Myerstown, PA
     CERCLA enforcement lead
               Christopher Corbett
               (215)597-8186
               Federal organization
            Vadose soil (benzene, 0.009 mg/kg; aniline, 0.009 mg/kg;
            PCE, 0.051 mg/kg; TCE, 0.017 mg/kg)
            Saturated soil (benzene, 0.002 mg/kg; aniline, 0.002 mg/kg;
            PCE, 0.012 mg/kg; TCE, 0.004 mg/kg)
            Other contaminants: arsenic
            Volume: 4,000 cubic yards
            % of total vol. at site: less than 10%
                         Full-scale remediation is planned. Currently in
                         predesign. Laboratory-scale and pilot-scale studies
                         are planned.
                                                                                                         Ex situ treatment, biological treatment.
  IV
     American Creosote
     Works—Jackson
     Jackson, TN
     CERCLA fund lead .
               Femi Akindele
               (404)347-7791
               Federal organization
            Soil (creosote; PCP)
            Other contaminants: chromium (+3), copper, silver
                         Full-scale remediation is planned. Laboratory-scale
                         and pilot-scale studies are planned.
Ex situ land treatment. Aerobic conditions, indigenous
organisms.
                                                                                                                                                    gl
b Indicates a new site.
0 Indicates that the site has been updated.
Shading indicates a non-CERCLA site.
                                                                                                                                                    3'
                                                                                                                                                    I
                                                                                                                                                    3!

-------
       FIELD APPLICATIONS OF BIOREMEDIATION (cont)
REG
IV
IV
IV
IV
IV
SITE/
LOCATION/
LEAD
American Creosote
Works — Pensacola
Peusacola, PL
CBRCLA fund lead
Batch/Slurry Reactor
In situ ground-water
bioremedialion
Land Treatment
Baldwin Pole and Piling "
Company, Inc. "
Bay Minette, AL
•kCRA lead (state)
BaumgartnerOjl1"
CharloMe, NO," "
State of Noah Carolina .
Brown Wood Preserving
Live Oak, FL
CERCLA enforcement lead
Cabot Koppers
Gainesville, FL
CERCLA enforcement lead
CONTACT/
PHONE
NUMBER
Mark File
(404)347-2643
Federal organization
Tom McMillan
(205)937-2141 "
Industry ' ^
-! N
Gary"Long ^ <
{708)468-2213 "
Industry
Martha Berry
(404) 347-2643
Federal organization
Martha Berry
(404)347-2643
Federal organization
MEDIA/
CONTAMINANT
Soil (PAHs; PCP)
Basis: risk-based
Other contaminants: dioxin
Ground water (benzene, 91 ppb; acenaphthene, 9,000 ppb;
fluoranthene, 1 ,500 ppb; dibenzofuran, 44 ppb; naphthalene,
21.9K ppb; PAHs, 1,100 ppb; PCP, 296K ppb)
Basis: alternate cone, limit (ACL)
Other contaminants: possibly zinc and dioxin
Volume: 1 52,000,000 gallons
% of total vol. at site: 100%
Soil (PAHs; PCP)
Basis: risk-based
Other contaminants: dioxin
Ground water (KOOI) *,« " "
Water {grease, 1 00 mg/U PCP, 0,96; phenol, Sj olC
100 rng/L; TOC; total arsenic," 0.044; total chromium,
,0.0895; toad CaA024) N < : ;
Basis; gro«nd-waterMCLoi-MDLjf:tt)«e|sfto MCL >
established ^ ^ *-
Volume: 20,000 galiortsj^r  N" ^ ^ •• * - ;. x
" ^ ^
\ "s^s ,1 ',^^
Full-scale remediation was completed 12/91.
Started 10/88.
Full-scale remediation is planned. Laboratory-scale
studies were completed 04/93.
Pilot-scale studies are planned.
TREATMENT
Ex situ treatment, sequencing batch reactor, slurry reactor,
batch flow. Aerobic conditions, indigenous organisms.
In situ treatment, ground-water bioremediation. Hydrogen
peroxide, nutrient addition (nutrients not yet determined).
Aerobic conditions, indigenous organisms.
Ex situ land treatment. Aerobic conditions, indigenous -
organisms.
Ex situ treatment, iixedlilm, completely mixed flow. Aeiobie-
conditions, indigenous 'organisms,
In sint treatmenf , isoji bipremedmtton (in stta land treatmenfJA
'Ex. situ land treatment. AeroMs conditions, indigenous,
-organisms. - '^v " v '
Ex situ land treatment. Aerobic conditions, indigenous
organisms.
Ex situ land treatment. Aerobic conditions, indigenous
organisms.
Indicates a new site.
c Indicates that the site has been updated.

-------
              FIELD APPLICATIONS OF  BIOREMEDIATION  (cont.)
                                                                                                         Ex situ treatment, slurry reactor, batch flow. Aerobic
REG
 IV
   SITE/
LOCATION/
   LEAD
     Cape Fear Wood Preserving
     Fayetteville, NC
     GERCLA fund lead
CONTACT/
 PHONE
 NUMBER
               Jon Bornholm
               (404) 347-7791
               Federal organization
   MEDIA/
CONTAMINANT
            Sediments (PAHs, 3 mg/kg)
            SoiI(PAHs, 100 mg/kg)
            Ground water (PAHs, 14.4K |ig/L)
            Other contaminants: arsenic, chromium
            Volume: 2,600 cubic yards
                                                                                     STATUS
                          Full-scale remediation is planned. Currently in
                          design. Laboratory-scale studies were completed
                          01/90.
                          Pilot-scale studies are planned.
TREATMENT
  IV
     Celanese Fibers Operations
     Shelby, NC
     CERCLA enforcement lead
               Dewey Williamson
               (704)482-2411
               Industry
            Ground water (acetone; 1,2-DCE, 0.07 ppm; ethylene glycol,
            7ppm)
            Basis: state MCLs
            Other contaminants: lead, chromium
            Volume: 93,500 gallons per day
                                                                          Full-scale remediation is being conducted.
                                                        Ex situ treatment, sequencing batch reactor, batch flow.
                                                        Aerobic conditions, exogenous and indigenous organisms.
                                izatton-,;
11 Indicates a new site.      ,. :,  •
0 Indicates that the site has been updated.':
Shading indicates a non-CERCLA site.
                                                                                                                                                    1

-------
                   FIELD APPLICATIONS  OF  BIOREMEDIATION (cont.)
 REG
        SITE/
     LOCATION/
        LEAD
CONTACT/
  PHONE
 NUMBER
    MEDIA/
CONTAMINANT
                                                                                                        STATUS
               TREATMENT
  IV
OwrfeHori Air Force Bait'-'
SS-41,FT-03,ST-I>
Charleston, SC
Federal facility
FT-03
       SS-4J
       ST-27
                              Marty Faite
                              (210)536-4331
                              Federal organization
              Soil (Jjeozenej elfjylbenzene; toluene: TRPHs; xylene)
              Basis: stale regulations
              Ouier contaminants: tad
                                              Soil (benzene; ethylbenzene; toluene; TRPHs; xylcne)
                                              .Basis; state regulations
                                              Other contaminants: lead
                                              Soil (benzene; ethyjbenzene; toluene; TRPHs; xylene)
                                              Basis: state regulations
                               Full-Kale remediation is planned. Pil
                               studies hive been underway since 11/9Z
                                                                                   Full-scale remediation is being conducted.
                                                                                   Pilot-scale studies have been underway since
                                                                                   11/93.
                                                                                   Full-scale remediation is planned. Pilot-scale
                                                                                   studies have been underway since t) /92,  „
In situ treatment, bioventing. Aerobic conditions, indigenous
organism!.
                                                                                               In situ treatment, hioveming. Aerobic conditions, indigenous
                                                                                               organisms.
                                                                                               In situ treatment, bioventing. Aerobic conditions, indigenous
                                                                                               organisms.
  IV
Coca-Cola0
Atlanta, <3A
                              ). LeotardJl-edbettet
                              {404)421-3569  -
                              Contractor/
              Vadose soil (pettotam oils, SOOtng/kg)
              Basis: Georgia UST tegulattons
              Worse; 630 cub. ic yar   "  ,
                                  x
                               Fall-scale remediation i*as completed 12/93,
                               laboratory-scale studies have been completed.
Irt site treatment, wtodrotfs-'-afitei: bioremedtattojt—reused
so!i.Natrierttaddttio»(SAI'ESOlL). '            •-
  IV
       Coleman-Evans
       White House, FL
       CERCLA fund lead
                       Tony Best
                       (404)347-2643
                       Federal organization
              Soil(PCP,25mg/kg)
              Other contaminants: arsenic
              Volume: 27,000 cubic yards
              % of total vol. at site: 100%
                               Full-scale bioremediation is not planned.
                               Laboratory-scale studies have been completed.
Ex situ treatment, slurry reactor, batch flow. Aerobic
conditions, exogenous organisms.
  IV
       Dubose Oil
       Cantonment, FL
       CERCLA enforcement lead
                       Mark File
                       (404)347-2643
                       Federal organization
              Soil (PCP, SO mg/kg; benzene, 10 mg/kg; DCE, 0.07 mg/kg;
              PNAs, 50 mg/kg; TCE, 0.05 mg/kg; xylene, 1.5 mg/kg)
              Basis: risk-based
              Volume: 20,000 tons
              % of total vol. at site: 46%
                               Full-scale remediation has been underway since
                               11/93. Laboratory-scale studies were completed
                               11/88. Started 01/88.
                               Pilot-scale studies were completed 06/94. Started
                               11/93.
Ex situ treatment, pile. Aerobic conditions, indigenous
organisms.
b Indicates a new site.
c Indicates that the site has been updated.
                                                                                                                                                                                   |

-------
                FIELD APPLICATIONS OF BIOREMEDIATION  (cont.)
REG
   SITE/
LOCATION/
   LEAD
CONTACT/
  PHONE
 NUMBER
    MEDIA/
CONTAMINANT
                                                                                           STATUS
             TREATMENT
  IV
     Escambia Wood Preserving
     Site—Brookhaven
     Brookhaven, MS
     CERCLA fund lead
                De'Lyntoneus
                Moore
                (404)347-3931
                Federal organization
             Soil (PAHs; PCP)
             Basis: risk-based
             Volume: 200 cubic yards
             % of total vol. at site: 1 %
                           Full-scale remediation is planned. Pilot-scale
                           studies were completed 11/92. Started 09/91.
Ex situ treatment, white-rot fungi treatment. Aerobic
conditions, exogenous and indigenous organisms.

  IV
      Mathis Brothers/South
      Marbletop Road Landfill Site
      Kensington, GA
      CERCLA fund lead
                Charles L. King, Jr.
                (404)347-2643.
                Federal organization
             Soil (benzene, 0.014; bis(2-ethylhexy!)phthalate, 40.4K;
             dicamba, 1,532)
             Basis: risk-based
                           Full-scale remediation is planned. Currently in
                           predesign. Laboratory-scale studies have been
                           underway since 04/94.
                           Pilot-scale studies are planned. Expected
                           completion 10/95.
In situ treatment, soil bioremediation (in situ land treatment).
Nutrient addition, batch flow. Aerobic conditions, exogenous
organisms.
b Indicates a new site.   '-"  -.  ,
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

-------
                   FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
 REG
         SITE/
     LOCATION/
        LEAD
   CONTACT/
    PHONE
   NUMBER
                  MEDIA/
              CONTAMINANT
              STATUS
               TREATMENT
  IV
       Minit Mart SIOO/Keystop
       nos*
       Lawrcnccburg, KY
       UST lead (state)
                        William Eddins
                        (502)223.9968
                        Contractor/
                        engineering firm
                 Vadose soil (BTBX, 1 mg/kg; PAHs, 1 mg/kg)
                 Ground water (BTEX, 5 ppb; PAHs, 5 ppb)
                 Basis: detection limit
                                             Full-scale remediation was completed 06/92.
                                      In situ treatment, ground-water bioremediation, soil
                                      biorcraedialion fin situ land treatment). Nutrient addition (type
                                      unknown). Aerobic conditions, exogenous organisms.
  IV
       Naval Sub Base
       Kingsbay, GA
       RCRA lead (state)
                        John Gamer
                        (912)673-8845
                        Federal organization
                 Ground water (2-butanone; 1,4-dichJorqbenzene, 75 Jig/I,;
                 1,1-DCA; 1,2-DCA. 5 (ig/L; 1,2-dichloropropane, 5 u.g/L;
                 4-methyI-2-pentanone; benzene, 5 ng/L; bmylmethylketone;
                 chlorobenzene; cis- 1,2-DCE, 70 u.g/L; ethylbenzenev
                 700 (ig/L; iron, 3 mg/L; TCE, 5 ng/U tetraehloroetbene,
                 5 ng/L; toluene, 1,000 fig/L; total xylenes, 10K (ig/U
                 transtl^-DCE 100jig/L; vinyl cbloride,2ng/L):
                 Other contaminants: site is still under investigation.
                 Volume- 86,400 gallons
                 % of total vol. at site: .03%
                                             Full-scale remediation is planned. Pilot-scale
                                             studies have been underway since 03/94.
                                      Ex situ treatment, fixed film. Aerobic conditions, exogenous
                                      organisms.
  rv
       Orfcift Facility
       Fort Pierce, PL
       RCRA lead (state)
                        Bob Hines
                        (404)888-2195
                        State organization
                 Soil (cMordane;lieptachk>r)
                 Basis risk-based
                 Volume: 750 oubjfe yards
                 % of total vol. at site. 100%
                                             Full-scale remediation is planned. Currently in
                                             predesign laboratory-scale studies nave been
                                             underway Since 03/93,
                                             Pilot-scale studies are planned.
                                      In situ treatment, soil bioremediation (in situ land treatment).
                                      Oxygen source) nutrient addition Aerobic conditions,
                                      exogenous organisms,  '
  IV
Patrick Air Foree-Base"
Patrick AFB,FL  ,
Federal facilityx
JBX Service Station'

FTA-2 "     \  —
                               Marty Faile
                               $10)536-4331
                               Federal
                 Soil (benzene, 100 Jig/kg; erhylbenzene, Itt) Hg/kgp toluene,-
                 100tlg/kg;TRPHs, lOnig/kg;xylenk 100|ig*g)  ';   C
                                             studies have be«n underway since 04793.
                                                                                   In situ treatment bioveatiag. Aerobic conditions, indigenous
                                                Soi! (beawne, 100 flg*g; e&yjbenzene, _iOO fig/kg; toluene,
                                                100 jig/kgj TBPf& 10 nig/kg; xyleae, 100 Mg/kgK ^
                                                                                                                            to situ treaaaent, bioventing. Aerobic" conditions, jndigeaoas
                                                                                                                            organisms^      «          - s     „    N,"    " % •
  JV,
Robms Air Force Base1*"
Robing AFB.QA
Federal facility  v
SS-JO :           '

OST-17S  '
LtrColRossMiilef
~<210) 536-4331  .  '
Soil ^(benzBne; etfeylbenzene; toluene; TRPHs; xylene)
FultscateTeiBedlation irpIanneA Pilot»seate
itadie^ have beeitt uinfe-way sine* 09/92,
In situ treatment, btoventiiigi Aerobic condi|3ons,,indigeno«s
                                               Soil (benzene; ethylbenzenej rofesne; TRPHs; xylene)  .
 Indicates a new site.
c Indicates that the site has been updated.
                                                                                                                                                                                        I

-------
          FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
                 CONTACT/
                  PHONE
                 NUMBER
                                                ;l^||i^rKra^^^r^^iM^.x_ v»* Avl'_^xir_l^Y<^i,lf-.i Jf.i;ji1'i_' /^^^^•^.•^IiJ'is?^*.*: J^fS^^i!"
                                                                                                 5'
                                                                                                 f
b Indicates a new site.
0 Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

-------
       FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
IV
IV
:IV
IV
IV
1 \
*° > .
w
<4
ivr
.JY
\
SITE/
LOCATION/
LEAD
Shavers Farm
Lafayette, GA
CERCLA fund lead
Shaw Air Force Baseb
SbawAFB.SC
Federal facility
Silvex
Saint Augustine, FL
State Lead
Southeastern Wood Preserving
Canton, MS
CERCLA fund lead
Southern Wood Piedmont1*
Baldwin, FL
RCRA lead {federal)
v * , »
< ^ x
~ x
SS-10, Air Force Plant 6,
Marietta*
Maaet»,6A " *,
•Federal facility
-StaUwortn Timber
Beatrice, AL -
RCRA lead(fedeK«\RCRA
Jead{state)
SfaJlworfii Timber^ (
Company, loo- <
Beatrice, AL „ v
RCM lead (state)'
CONTACT/
PHONE
NUMBER
Chuck Eger "•*' '
(404)347-3931
Federal organization
U. Col. Ross Milter
(210) 53^331
Federal organization
George Heuler
(904)488-0190
State organization
Don Rigger
(404) 347-3931
Federal organization
TM Oavis
(803)599-1070
Industry
x ° v
x. ^
<. ~
^ X N * -v N
S *•"
*,
J v ' ^
Lt Col Rosslfflef
<210) 536-4331 ^
Federal organizattoa'
JasoitDarby N
(404)347-3453^ o
Federal orgaaizattojt
B% Thames
(3&5)789-ai5l ;
Industry v
MEDIA/
CONTAMINANT
Soil (benzoic acid, 25 mg/kg; benzonilrile, 25 mg/kg;
dicamba, 25 mg/kg; dichlorosalicyclio acid, 25 mg/kg)
Soil (benzene; ethylbenzene; toluene; TRPHs; xylene)
Basis: state regulations :
Ground water (acetone, 700 ng/L; benzene, 1 ng/U
2-butanooe,680(ig/L;i*loroforin,0.7ng/L; i,l,l-TCA.
-200 (ig/L; 2i4-dimethy!phBnol. 39 ng/L; cresols, 700 |Jg/L;.
:etbylbenzene, 39,)tg/L;methyIene chloride, 7pg/L;.MIBK,.
350fig/L; toluene, 2,000 }tg/L)
Other contaminants: oadrnium, chromium, copper, lead,
nickel, silver, zinc
Soil (PAHs, 180 mg/kg; total PAHs, 950 mg/kg)
Volume: 8,000 cubic yards
% of total vol. at site: 1 00%
Ground w&ter (acenapluhene, 20 (ig/L; anthracene; BAP,
02pg/L;-2-chIonjphend;henzo{a)atttnracens, "" ;
2,4«dichtorophenol, U&rog/L; 2,4-dimethylphenol,
2,4-dimtropheaol, 70 jigS4l;eitzS{b)fluoratithene;
berawKfcjJtuorantfiene, Z^&tetrachloiopheno!, 350 ftg/L, "
2,46-tnchipropfeenol, 1,8 (i^L tfirysefc creso), JSOMg/l).
dibeazo{a,h)aiaiiacene;fliiorant&ene>190-{t|/L;v v
j»deBo£!,,2,3-cd)pyceiie^aaphthafeiie, ^900 jtg/L,
p-cbt^1)JMe»l,K;hl)jg/t;i)ijenanthFeiie,20tlga' X
pheao^l,4BO]£g/L;WtoeBeJ,OOOpga.) \ >,
Other oontaiwnrots^okomiHmawJ arsenic ^
-Soil (benzene, ethylbenzene; toltieae; TRPHs; xylene) •> j
Sa?is.s(at«,reg«laions ^ \
Sail/ground water (cteosliJe.PCP)- , ^
1 t \ \
*i \
Vadose and safiirated soil (KjP, PAHs, beB2Bne;s s v
jiaphthaiene,«te}
Basis-MdL , "' s '" " \ t xv, ,
^ ^ \ ' \ 5 *
-• . , * x •>•
STATUS
Pilot-scale studies have been completed.
Full-scale remediation is planned. Pilot-scale
studies have been underway since 06794.
Full-scale remediation has been underway since
10/93J Laboratoty-scale studies were completed
08/91, StartedOl/91.
Pilot-scale studies were completed 1 0/92. Started
01/92.
Full-scale remediation was completed 08/94.
Full-scale remedtaUoa is planned. Currently being
installed JUIwratory-soafeshidiesliavebeen
completed
\ ")
x s ^ ' *
i *, *. * c ° s ~
•• ^ N ^ ^ V-
Full-scale retaeStafioa Is jjlaaned. RJot-iScale
studies were completettl»93 Started 11#3 \
\ *
\ ^ v ->
Fall-scale remedtaton is jrfaaned Gutre»!ly in }
predesjga, •• . v l <,
\ \ ^ H ^ a
Fultscate remedtaflO-n is pfenned. Ciataatly in
predestgn Pilot:-8*»to«n!dfesareplataisd»
< " ' X ^ X ' ' x \;
N - "V Xx
C. \ >
TREATMENT
Bioremcdiaiion [reatment not yet established.
In situ treatment, bioventing. Aerobic conditions, indigenous
organisms. '
Ex situ treatment, fixed film, completely mixed flow. Aerobic
conditions, exogenous organisms.
Ex situ treatment, slurry reactor, batch flow. Aerobic
conditions, indigenous organisms.
In slta, treatment, ground-water bferemechation Oxygen
source, nutrient addition. Aerobic conditions; Indigenous
organisms ' *
x * ""
* N ^ *X \ N ^
^ 'sC^ * . * o * * * ' \ V
"v" ^ °~o^'v
In sifti treaanent, bioventingxAerof>ic conditions, indigenous
organisms
In situ treatmeaCsoilbiOKmediatJon {in stta-tand treatment)
JBx siw jreatment, &tfvate4 stiidge, ceroptetely mixed ffow^
Aemtae cSadiSofts^exogeneas ancTsadigeaoMs orgaats»s.
' ^ s ' ,
•^ xx
In site tteatmeBt soil bioremeolatjoa (in siftrlanti treatment).
^Oxygen sourcft, juttrtenraddiiion 
-------
                FIELD APPLICATIONS  OF BIOREMEDIATION (cont.)
 REG
  IV
   SITE/
LOCATION/
   LEAD
      White House Waste
      White House, FL
      CERCLA fund lead
CONTACT/
  PHONE
 NUMBER
                Tony Best
                (404) 347-2643
                Federal organization
    MEDIA/
CONTAMINANT
             Soil (BAP, 0.192 rag/kg; 1,4-dichlorobenzene, 0.012 mg/kg;
             benzene, 1.13 |ig/kg; 2-methylnaphthaIene, 2.2 mg/kg;
             chlorobenzene, 970 mg/kg; di-n-butyl phthalate; methylene
             chloride, 29.3 mg/kg; naphthalene, 0.261 mg/kg; PCB 1260,
             0.09 mg/kg; PCE, 4.3 mg/kg; phenol, 0.549 mg/kg; TCE,  •
             0.0447 mg/kg; toluene, 14.4K mg/kg)
             Ground water (acetone, 0.0016 mg/L; BAP, 0.0002 mg/L;
             benzene, 0.005 mg/L; bis(2-ethylhexyl)phthalate,
             0.004 mg/L; carbon disulfide, 1.64 mg/L;
             2-methyInaphthalene, 0.067 mg/L; di-n-butyl phthalate;
             ethylbenzene, 0.7 mg/L; m-cresol, 0.85 mg/L; MEK,
             8.46 mg/L; naphthalene, 9,700 mg/L; p-cresol, 0.85 mg/L;
             phenol, 10 mg/L; TCE, 0.005 mg/L; toluene, 1 mg/L; xylene,
             10 mg/L)
             Other contaminants: lead, other inorganics
             Volume: 56,000 cubic yards
             % of total vol. at site: 100% of pit material
STATUS
                           Full-scale remediation is planned. Currently in
                           predesign. Laboratory-scale studies were
                           completed 09/91.
                                                                                                                            TREATMENT
                     Ex situ treatment, slurry reactor, batch flow. Aerobic
                     conditions, exogenous organisms.
b Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

-------
        FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
V
V
\
V
• v.
; -
V,
V
SITE/
LOCATION/
LEAD
Alien* West Roads*
Woodhavcn.Ml
3;07 State Lead
Allendale Shell"
AJleadale,MI:
USTIeadXstate)
Allied Chemical
Ironton, OH
CERCLA enforcement lead
Ex situ magnet, enh. bio
In situ bioremediation
Land treatment
XmenfecMMichigan x > s •
Bell— Holland* ;s - N*
Hofland/Mt „< ,
yST lead (state) -, -'
Araerrtech/Michigan s ^ N <
Bell— Cotnstock Park* \ ^-s
Comstock Park, MI
USTJearf (state)
Amoco 80012'X "" x-
Roekford, MI - v"^ -v "
TJST lead (state) %
CONTACT/
PHONE
NUMBER
Michael B. Stenzel
(517)373-0249
Stale organization
Michael B. Stenzel
(517)373-0249
Slate organization
r
Tom Alcamo
(312)886-7278
Federal organization
Michael jMfenzeT
qi?) 373^0249
State organization xs
WfcnaelB.Straizel "
(517)373-0249 "
State organtzati,oir <
Michael B, Stenzel
(517)373-0249 ^ "
^tete organization^ X
MEDIA/
CONTAMINANT
Soil (aroclor 1248, 1,000 ug/kg; aroctor 1260, I,000(tg/kg:
benzene, 24 ug/kg; BAP, 330 ug/kg; benzo(a)anthracene,
330 ug/kg: benzo(b)nuonintfaene, 330 ug/kg; chromium;
cbrysene, 330 Ug/kg; dibenzofuran, 330 |ig/kg; etbylbenzcoe,
1^00 (ig/kg: phenanthrene, 500 ug/kg: TCE; xylene,
5,600 ug/kg)
Basis: risk-based
Other contaminants: metals
Soil (benzene, 24 (ig/kg; etbylbenzene, 1,500 ug/kg; MTBE,
4,600 jig/kg; xylene,:5,6C(p ug/kg)
Ground water (benzene, T.2 ppb; ethylbenzene, 74ppb;
MTBE, 230 ppb; naphthalene, 250 ppb; toluene, 790 ppb;
xylene, 280 ppb)
Basis; risk-based , . -
Sediments (PAHs, 1 ppm)
Basis: risk-based
Other contaminants: arsenic
Volume: 1 ,000 cubic yards
Sediments {PAHs, 1 mg/kg)
Basis: risk-based
Other contaminants: arsenic
Volume: 460,000 cubic yards
Sediments (PAHs, I ppm)
Basis: risk-based
Other contaminants: arsenic
Volume: 30,000 cubic yards
Soil (Benzene, 24 (tg/kg) / ^_ • -, ^ \ ' ,
Ground water ftenzsne, 1 2 ppi* ethylbeteene, 74 ppfr; _
MTBE, 230 ppb; toluene;, 7SO ppb; xytene,2|0j>pb) „ "x
Bastsfrisk-oased i > - \x v - >
<3K)lh3d"watet;(benz;ene^ 1.2 ppb;MTSB,230ppbrxylenB,
Sasisilisfebased. x- t "• ^ A x ^ ^N" - ;
(Sround water (tjenzene, !,.2 ppb) > \ * -v
STATUS
Full-scale remediation has been underway since
12/92. '
.Full-scale remediation has been underway since
.03/95,
Full-scale remediation is planned. Currently in
design. Laboratory-scale and pilot-scale studies
have been completed.
Full-scale remediation is planned. Currently in
design. Laboratory-scale studies were completed
09/92.
Pilot-scale studies have been completed.
Full-scale remediation is planned. Currently in
design. Laboratory-scale and pilot-scale studies
have been completed.
Hull-scale remediaB'bn.!»:> been umterway'stnca*
06/94.xLaboratory-scafe studies were completedx'
"Q6/9\ Started OU94,^ -' ,. 'V
s ^»ll-Scale.remed&don ha^ been updserway^ince"
^0!^4. . "-^ \ - < ' XX V"^N •• r ^ ^i
s ^ ) x N n^ % \ < X :
Full-scaSfcreniediatioiiaas been underway sincse ^. ".
TREATMENT
Ex situ treatment, ex situ soil biore mediation. Anaerobic
conditions, indigenous organisms.
In situ treatment, ground-water bioremediation, soil
bioremediation (in situ land treatment). Ejt situ treatment,
fixed film. Oxygen source, nutrient addition (nitrogen and
phosphorus (5:1 nitrogen and phosphorus)), completely mixed
flow. Aerobic conditions, indigenous organisms.
Ex situ treatment, reactor used to grow microorganisms where
a magnetic field is applied, then circulated to two engineered
cells. Aerobic conditions, indigenous organisms.
In situ treatment, sediment bioremediation. Oxygen source,
nutrient addition. Aerobic conditions, indigenous organisms.
Ex situ land treatment. Aerobic conditions, indigenous
organisms.
Jn situ treatment, soil 6laremediatJQHstreatnienr) . '•
Oxygen source. Aarobitfcoa'dttioas; indigenous organism^
'AerobKfcondittans.'inmgenousorgaittsras. * » \ ,.'
Anaerobic cond^ioas,NiadtgeBO»s organisms^ - _
N>
 b Indicates a new site.
 c Indicates that the site has been updated.

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           FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
 REG
  SITE/
LOCATION/
  LEAD
CONTACT/
 PHONE
NUMBER
   MEDIA/
CONTAMINANT
STATUS
TREATMENT
 -••y;;

                                                                           V "^-^v'* ^ ^A^.v'r^^ ;J;.~/ >^^,yv ^ r V-^^^S-W< -V ^-v
                                                                           •In siHt,treatnjenf, aixspatgingj-grouna-water-bioreraedianqn, .• -
                                                                            '''"^
                                                                           •spit'bto^'i^weoftji«|to'j^"tt^ne8^iOx5?g^^^
                           !CKs|^'v^^^^^i|Sipj^c^fe^«^
 Indicates a new site.
u Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

-------
       FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
V
V
V
,v.
J X.
v"
X <
v»,
V x
fY>~
<.
SITE/
LOCATION/
LEAD
Aristecfa Chemical
Haverhtll.OH
RCRA lead (federal)
Bioventing
Land treatment
AT&T Love!) Street6
Kalamazoo, MI
WST lead (state)
Autostyle
Kentwood, MI
CERCLA enforcement lead
AutoatyteWaltics* " \ * *
^307 State Lead * •
B&J Country Store ^
'Jntee Oaks, 7^d ^ *> >
\JSttead (state) < x *
<*-*"-. " ^ ^ \ x
B&PTruekragCompany x"
Rochester,-MN ^ , -•
USTleati (state) -\ ,
«-> x x-" x%-
•$07 State tead
sxx *
B&^Co=OJ»l)t * \°
Breck«nndge,vMI ,<
ySTfe8
„ ^-\
X 'X
State organization •>
(5r?) 373-0249 "''
_ v
MEDIA/
CONTAMINANT
Vadose and saturated soil (phenol. 10 rag/kg)
Basic: risk-based
Other contaminants: arsenic, barium
VoluiW: 10,000 cubic yards
%o( total vol. at site: less than 1%
Vadose soil (phenol. 1 0 mg/kg)
Basis: risk-based .
Other contaminants: arsenic
Volume: 1,200 cubic yards
® of total wt at site: less than J %
Sotl(benzo(a)anthracene, 180 fig/kg; BAP, ISOng/fcg;
benzo(6)flttoran(nene, ISO [tg/kg; pheriawnrene, 500 ng/kg)
Ground water (benzene, 1.2 ppb; ethylbenzene, 74 ppb;
xylene,280ppb)
Basis: risk-based
Ground water (alcohol; aromatic ketones)
Giants} water (acetone, ethylbenzene; isopropaao); MEK;
i,l,J-TCA>T(>etbylene«1itoride;i1C3B,TCE;tolue8e) " !
Basi^: risk-base^ ,, N ^ '•> \
vSoil°(beBzeMe, 24 jig/kg, ethylbenzens, 1 ,500 fig/kg; xylene, ^
5»6J00_4tgft:g) x ., < ^ xx- » , ,,
Ground water (beazeae, ! 2 pj)b) *^ * ° ,-
Basis dsk-based • - s • « «x
Soil tBTEXittse oil, 50'mg^) ^ ^ . °l
Ground WftterlBirBX tabs oil) ^xv ^ * , .
_ Basts tsotl}-iislt:4iased N ' ^*
Basts (ground water), 100- x MN Dept of Healtb
%^of total voj, &t$lte* 75%° ° * ^ x x
s X "~ x. x
X * 'X
Orouttd water (V,2rBd, iOO ppb.5 nwhy)sAe.ehloride, 5 ppb;
UJ-TCA^OO^rii^f.J'DCA 700ppb, 1>Vl>CB, 7 ppb, x
1,2-DCA, 04 |>gb' TCE, 3 ppb; v»yJ crteide^OJS ppb)^
Basis/'nsk-baseti « -
i X X ( •.
Other ^ontatfitnants heavy me^ls v xv- ^
SoI^benzeiK, 24 (Jg/k® eJbylbenzene, tJOO fi^kg1,
xuar*tnalea6,x5,OOOng*r^y]en«,5,Wftg/kg) » - •;

STATUS
Full-scale remediation has been underway since
07/94, Laboratory-scale studies were completed
,06794. Started 05/94. ' ' -
Pilot-scale studies have been completed.
Full-scale bioremediation is not planned.
Laboratory-scale studies have been completed. _;:;
Full-scale remediation is planned.
Full-scale remediation has been underway since
09/90. Laboratory-scale and pilot-scale studies
have been completed.
Fall-seaferewediatio^ has Seen wfltenyay since "
06789. Pilofr-s^file studies have been. uttd£fWay
sinctfQl/SS ^ x ^ *
X X ^ X*x >
JEWl-Scate remedktion bas))e^n underway since
0!$4. x , N
Fall-scale remediation was completed 12/92. x
Started 04/91 yboratory-seafe studies were x ^
completed 0130, x x ^ ^ ""^ ^~v x\ ,xx"x
Pilot-scale! stuaie^Kave been Completed.- ;
"l •- v ^ x " s
v p x x s
X X ^ x ^
^ ^ * *- ^
&iM-s$ale remedtaliQnls plaanea. Laboratory-scale
and tjOot-Scale; studies: a» Manned ; ^x
' v " " X% , \ O " - "-
Fyll-scafc retnedtalioJth^beffli'underway-sJrtfife
OJ/93 .pilot-scale studies ha^eteen coMfeted
X * ^
N ^ x^ * :
TREATMENT
In siiu treatment, bioventing. Oxygen source, nuiricnt
addition. Aerobic conditions, indigenous orgam'smi.
In situ treatment, soil bioremediation (in situ land treatment).
Nutrient addition (nitrogen-phosphorus slurry). Aerobic
conditions, exogenous and indigenous organisms.
Aerobic conditions, indigenous organisms.
E\ situ treatment, fixed film, completely mixed flow. Aerobic
conditions.
lx%tu tnatraent, fixed Sim, batch fiawwtoobic condittqni x
indigenous orgahSsrns, i t * - \H »° <•
X < o xv v v
Anaerobio condi&ons, indigenous organisms^ -* xx Nx,
x ^ ^ *• < s
X x v " x ^ i 3
X ' x e ^
^XiSita^tisatment) sfifjnenemg 6atch reactor, compfete^y nasest
lfeT¥,Xerpkc«c«dirf(>ns,inaigenw)3.orgaiBSTOS^' '
\ V X
X ' X 0 '
^ „ ^ X *"
^ f vx **
Bior^meiliationtteattBeatttoty'etesfabiisheii. ^" v <•
X  ^ "' \, * - N N" x > ^ V XX
^ xx ^ •> x x , ^
XX».X X^^ X ^,, ^X
Ex site jreatmentpUe, Oxygen sourc*, marjeftt addition £eow
and horse tnaaare) Aerobic conditions, indigenous organisms.
x \ > ^ -0- *
b Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

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              FIELD  APPLICATIONS OF BIOREMEDIATION (cont.)
REG
   SITE/
LOCATION/
   LEAD
CONTACT/
 PHONE
 NUMBER
   MEDIA/
CONTAMINANT
                                                                                STATUS
           TREATMENT
     Bendix Corporation/Allied
     Automotive Site
     St. Joseph, MI
     CERCLA enforcement lead
     Fixed-film reactor

     In situ ground-water
     bipremediation
              John Kuhns
              (312)353-6556
              Federal organization
           Vadose soil (DCA; TCE)
                           Ground water (DCE; DCA; TCE; vinyl chloride)
                        Full-scale remediation is planned. Currently being
                        installed. Laboratory-scale studies have been
                        completed.
                        Pilot-scale studies are planned.
                                             Full-scale remediation is planned. Laboratory-scale
                                             studies are being conducted.
Ex situ treatment, fixed film, plug flow. Aerobic conditions,
indigenous organisms.
                                                     In situ treatment, ground-water bioremediation. Aerobic and
                                                     anaerobic conditions, indigenous organisms.
b Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.
                                                                                                                                           I

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                       FIELD  APPLICATIONS  OF  BIOREMEDIATION (cont.)
    REG
    SITE/
LOCATION/
   LEAD
CONTACT/
  PHONE
 NUMBER
     MEDIA/
CONTAMINANT
                                                                                                                     STATUS
                 TREATMENT
          Blodgeii Oil—St. Johns1'
          St. Johns, MI
          UST lead (state)
                    Michael B. Slenzd
                    (517)373-0249
                    State organization
                Soil (benzene, 24 ng/kg; Obylbenzene. 1400 ug/kg; xylenc,
                5,600 jig/kg)
                Ground water (benzene, 1.2 ppb; ethylbcnzene, 74 ppb;
                MTBE, 230 ppb; xylene, 280 ppb)
                Basis: risk-based
                Volume: 1,700 cubic yards
                                  Full-scale remediation has been underway since
                                  12/94. Laboratory-tcaJc studies were completed
                                  12/94. StartedOl/94.
 Anaerobic conditions, indigenous organisms.
          BlodgettOil—St Louis'
          S(. Louis, MI
          UST lead (state)
                    Michael B.Sten7.el
                    (517)373-0249
                    State organization
                Soil (benzene, 94 |ig/kg; etbylbenzene, 1,500 ng/kg;
                mcthylene chloride, 92 (lg/kg; naphthalene, 5,000 ug/kg;
                xylene, 5,600 ng/kg)
                Basis: risk-based
                                  Full-scale remediation has been underway since
                                  01/95. Pilot-scale studies have been underway
                                  since 01/95.
In situ treatment, soil bioremediation (in situ land treatment).
Oxygen source. Aerobic conditions, indigenous organisms.
          BoeveOilb
          Holland, MI.
          UST lead (state)
                    Michael B. Stenzel
                    .(517)373-0249
                    State organization
                Ground water (benzene, 1.2 ppb; ethylbenzene, 74 ppb;
                toluene, 790 ppb; xytene, 280 ppb)
                Basis: risk-based
                                  Full-scale remediation was completed 01/94.
                                  Started 12/93. Pilot-scale studies were completed
                                  12/92. Started 12/92.
 In situ treatment, air sparging, ground-water bioremediation
 Oxygen source. Aerobic conditions, indigenous organisms.
OJ
Os
          Boeve Oil (Mike's Mobil)"
          Holland, MJ
          UST lead (state)
                    Michael B. Stenzel
                    (517)373-024$ <
                    State organization
                Soil (BAP, 180 tig/kg; phenanthrene, 500 Ug/kg)
                Ground water (benzene, 1,2 pj>b; xyleoe, 280 ppb)
                Basis: risk-based
                                 Full-scale remediation has been underway since
                                 05/95. Pilot-scale studies werecornpleted (1/94.
                                 Started 11/94    ,s
In situ treatment, air sparging, ground-water bioremedjatibn,
soil bioremediation (in situ land treatment). Oxygen source.
Aerobic conditions, indigenous organisms.
          Boeve Oil—-Water Street^
          Allegan,MI    "
          USTfeadXstate)
                    MtchaelB. Stenzel
                    (517)373-024^
                    State organization
                GrounS water {benzene, 1.2 ppb; ethylbenzene, 74ppb;
                MTBB, 230ppb^acenaphthene, 25 ppb; acenaphthylene,s
                25 ppb; naphthalene, 250 ppb; pyrene320 ppb; toluene,
                790!ppf>;ky!ene,>280 ppb)      ^ -     •   ' "'
                                  Full-scale remediation is planned Currently in  \
                                  design. Pilot-scale studies were completed 06/94.
                                  Started06794..  . C  ."
 In afu treatment, air sparging, ground-water, bioretnedtation •
Oxygen source. Aerobic conditions, indigenous organisms,
          Boralo One Stop'
          Zealand, MI
          USTIead (state)
                    Michael _B. Stenzel
                    (5l7)37iQ249
                benzo(b)fluorant|iene, 330 Ug/kg; naphthalene,'' 5.0W Jig/kg;
               phenanthrene, 500 u.g/kg; toluene^ ioTC |ig*g; xylene,
                               N
                                  Fail-scafOTemedration basjbeen underway since
                                  04/95,. Pilot-iSCaleswdies''are planned, ,
In situ treatment," air sparging, ground-water biorernediation,
soil faioremediation (in situ land, treatment) Oxygen source.
Aerobic conditions, indigenous organisms.1
                                                      GrftUfld water {benzene>--l,2 ppb; MTBEJ 230 ppb,
                                                      ethylbenzene, 74 pj>b;^naphftiafeBe, 250 ppb; ioiueae, N
                                                     '           e; 280ppb) "  .  ,v  " -  ,   "'   -
                  CorporaSoil1'
          Kalamazoo^ MI   "-   s
          307 State'^sd ; s- N
          Process I

          Process 2
                    Michael B,,Stenzel
                    {517)373"-0249" 5>
                    State organization^
                Ground n?a«r (ICE, 2,2 ppb; naphthalene, 250 ppb; ,'
                l,2-DGAV0.38 ppb; chjbroathane, 9.1 ppb; PCB, 0.7 ppT>;
                                  '          ^
                                 Fu!UscalersJ«e<(&i(}
-------
            FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
IEG
  SITE/
LOCATION/
  LEAD
CONTACT/
 PHONE
 NUMBER
   MEDIA/
CONTAMINANT
                                                                          STATUS
                                                                                                      TREATMENT
    ^wr*^-'^.-^
    »'r-»A WAYfsifemlv.--:. '. i»
                                                                                    fi sittf ^atmih't'So4.biore^raiatfbn,flri jsita-Iand teattnent).-;' ;,i
                                                                                    ^^^i'^-^^^^dg^lisiS^^^^^Mc'^^tesf^'
    Burlington Northern—
    Minnesota
    Brainerd, MN
    CERCLA state lead, RCRA
    lead (federal) (CERCLA-state
    to start; RCRA-federal since
    1986)
              David Seep
              (913)661-7015
              Federal organization
           Vadose soil
           Ground water (PCP)
           Volume: 10,000 cubic yards
           % of total vol. at site: 100%
Full-scale remediation is being conducted.
Pilot-scale studies were completed 01/86.
                                                 Ex situ land treatment, nutrient addition. Aerobic conditions,
                                                 indigenous organisms.
b Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.
                                                                                                                                  CD

                                                                                                                                  £

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         FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
V
V
V
V
V
V
V
SITE/
LOCATION/
LEAD
Clark Gee Oil Company11
Pontiac,MJ
307 Stale Lead
Cliff/Dow Disposal Site
Marquette, MI
CERCLA enforcement lead
Coca Cola Foods
Paw Paw, MI
UST lead (state)
Conoco Wrenshall Refinery11
Wrenshall, MN
CERCLA state lead
Crystal flash— Westnedge*
KaJ^masx), Ml
USTtead {state}
QystsiFtasnSulk6
Rockfor^MI x
OST!ead(sia«)
<,
Crystal Flash— Jewell Bjdk ,
Facility* - \
LoweI,Wl ^
USTfead (slate) - '
CONTACT/
PHONE
NUMBER
Michael B.Slenzel
(517)373-0249
Slate organization
Ken Glatz
(312)8S6-1434
Federal organization
Michael B.Stenzel
(517)373.0249
State organization
Dale Trippler
(612)296-7746
State organization
Michael B.Stenzel
(-517)373-0249 •
Siate organization
Michael B.Stenzel
(517)373-0249
State.orgai»zgft0n v
^ •* ^
*
i
Michael B.Stenzfil
(517)373-0249 ' '
State orgamzatfoB x
MEDIA/
CONTAMINANT
Soil (cihylbenzene, 1,500 fig/kg; acenaphihykne, 500 ng%;
naphthalene, 5,000 fig/kg; xylene, S.600 ngflcg)
Ground water (benzene, 1 .2 ppb: ethylbonzene, 74 ppb;
MTBE, 230 ppb; phenamhrene, 25 ppb; pyrene, 520 ppb;
xylene,280ppb)
Basis; rule-based
Vadose soil (PAHs, 0.3 mg/fcg)
Basis: health-based
Other contaminants: arsenic, copper, lead, mercury
Volume: 9,000 cubic yards
% of total vol. at site: 90%
Ground water (benzene, 12 ppb; naphthalene, 250 ppb;
pbenanthrene, 25 ppb)
Basis: risk-based
Vadose soil (TPHs, 50 mg/kg)
Basis: indirectly risk-based
Other contaminants: lead in south surface
impoundment/south landfarm area
Volume: 52,800 cubic yards
% of total vol. at site: 80%
Ground Water (benzene, 1 2 ppb, ethyJbenzene, 74 ppb? \
naphthalene, 250 ppi); toluene, ?90ppbs xytene, 280 Epi>|
Basts; risk-feased* "-\ v % s <,
> s ** ^ *> ^
Sot) (bBKeaeUW-ug&g, ethyaenzens, f.sqOHgig, 4 *
ph,en««t!)reie,50(Jfigi!k|,«yfc!te,5,OT)Hgfl!:g) \ x
Orpund -watq-Xnapiitnateiw, 250'ppbj pheaantbtejw, 25 ppb, , :
xylene>^?0ppb) ^^ ' s *•; x ^ ' x >
Basts: risk-based * _ ' ^^ 5 ' i. x
VAlame(sai!J:30fiwbi«ya>lann8d. Currently be&g »
wstelted, lS, ^ " - > •- "• ^
> (, . ' S< ><' . / „
Full-scateremediaa£4 jias'been \ih
12&4 ^
< x\ % N; ' « . :
,- > ^., V $
TREATMENT
In situ ireatmeot, air sparging, ground-watei' bioremediau'oo,
soil bioremediation (in situ land treatment). Oxygen source.
Aerobic conditions, indigenous organisms.
Ex situ treatment, pile, forced air biological treatment (FABT).
Aerobic conditions, indigenous organisms.
Anaerobic conditions, indigenous organisms.
In situ treatment, air sparging, bioventing. Oxygen source.
Aerobic conditions, indigenous organisms.
Anaerobicx:ondittojjs, - - - ' I
3 S> ' <. *
S. •* *• ^ ^ 5-
ta Situ keatnient, ground-Water bloretnediatton, soil - ,
bioVemaaiatraH (In site Jarndtreatment). Oxygen source, <. "
^erobj(icc*d)tio»5,'i«aige«qu$^rgsmsins s » •• °
oo
oo

 Indicates a new site.
 c Indicates that the site has been updated.
 Shading indicates a non-CERCLA site.

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        FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
              CONTACT/
               PHONE
              NUMBER
  MEDIA/
CONTAMINANT
b Indicates a new site.
c Indicates that the site has been updated.
" Shading indicates a non-CERCLA site.

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        FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
V
V
V
V
V
V
V
V
V
SITE/
LOCATION/
LEAD
Falcon Manufacturing"
Byron Center, Ml
UST lead (state)
Farm Supply Service,
Inc.— Ca'rrallton
Carrollton, IL
UST lead (state)
Farm Supply Service,
Inc. — Springfield
Springfield, IL
UST lead (state)
Fennema Excavating6
Byron Center, MI ..
UST lead (state)
Galesburg/Koppers
Galesburg, IL
CERCLA state lead
Gaylord Repair Facility"
GaylofAMI
30? State Lead ,
General Motors Proving
Grotmds* ^
Md&Hf,Ml
UST lead (state)
\
o *
Genera) Motors Proving ' s
GrotuidsffiuiidifiglZ1* x
JSilford,MI
XfST lead (state)
Genesee Valley Mai!11 - * ,
Flint MI
307 State Lead \
CONTACT/
PHONE
NUMBER
Michael B. Stenzel
(517)373-0249
State organization
Craig Steinheimer
(217)782-6761
State organization
Craig Steinheimer
(2J7)782-6761::
State organization
Michael B. Stenzel
(517)373-0249
State organization
Brad Bradley
(312)886-4742
State organization
Michael B, Stenzel
(517} 373-0249 -
State organization
Michael B.Stenze) N
(517)3734)249
State organization
^ •* ' ^
Michael B Stenzel
(517)373-0249
State organization
Michael B.-Sfenzei
(517)373-0249
Sfate-orgamzation
MEDIA/
CONTAMINANT
Soil (benzene, 24 (ig/kg, ethylbenzene, 1,500 Jig/kg; xylene,
5.600 ng/kg)
Ground water (benzene, 1.2 ppb; ethylbenzene, 74 ppb;
xylene. 280 ppb)
Basis; risk-based
Vadose soil (benzene, 0.005 mg/kg; BTEX, 11.705 rag/kg)
Volume:3,!20 cubicyards
% of total vol. at site: 100%
Vadose soil {benzene, 0,005 mg/kg; BTEX, 1 1.705 mg/kg)
Volume: 2,200 cubic yards
% of total vol. at site: 100%
Soil (ethylbenzene, 1 ,500 ^g/kg; naphthalene, 5$X> (ig/kg;
xylene, 5,600 fig/kg) ,
Ground water (benzene, l,2ppb,ethylbenzetie«74ppb;
naphthalene, 250 ppb; toluene, 79,0 ppb; xylene-, 286 ppb)
Basis: risk-based , „
Soil (chlorophenol; PCP; PAHs; phenols; PNAs)
Soil(pherknthrene,''SOOngfl4;T|ps,'lOOw^kg) ^
Ground water (benzene,, i 2 ppb, naphthalene, 250 ppb;
phenanthteBe>25ppKTPHs,l"j!pm) \
Basts* risk-based ,, j
Soil (acenapifhytene, 400 Itjftg; phenanlhteae, 500 ugAgj '
henzo(a)amkacene,33&ilg/kg;l)enw)(g,y)pey!ene, N ' >;
330 Jig/kg; benzo(k)flilKWa(ifhene, 330pg#g; chrysede,
330 Hg/kg, pyrene, IOK Vs/tigl TPHs, 1,00 jng/kg| v ^ '
Basts*"risfc-based • > , \
Volitme 5,000 cubic yards x ss« ; s
%oftoMlv ^ ' *N ' ' tsX
Soil (toluene, idpg/kg'sxytene^lOitg/kg) ' . .
Basis: MDNRType,^.imm1)et$ (nonrisk-based)!
S<'"\,\V^' '"^ ^. •
Sod 5ben2ene>24(jg/fc|;e(hylbenj-ene,J,500pgfli:g*toIuene., .
16K}^Scg, xylene" 5,600 Hg£gV\ ,' , x -• -> " '
Basts- rtsk-bssed ^ , - v "\,> - 5 \<,
STATUS
Full-scale remediation has been underway since
09/93. Pilot-scale studies were completed 12/93.
Started 01/93.
Full-scale remediation was completed 12/93.
Started 10/92.
Full-scale remediation was completed 01/94.
Started IO/92';
Full-scale remediation has been underway since
01/94. Pilot-scale studies have been underway
since Ot/93.
Full-scale remediation is planned. Currently in
predesign.
Fui^ateremedfajionJsa^beetnintterway since N x
03/95
0 \
F»Il-scal^reme'diatJOTj"v?ascp?npIete "" '
\ A
\
JF«H-scaleiem«dta(ionwas c
-------
        FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
  SITE/
LOCATION/
  LEAD
CONTACT/
 PHONE
NUMBER
  MEDIA/
CONTAMINANT
                                                 STATUS
                                                                    TREATMENT

h Indicates a new site.    •
c Indicates that the site has been updated;
Shading indicates a non-CERCLA site.

-------
        FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
V
V
V
V
V
V
V
,i-%
•0?
;-,\~V
£•?
"•*" ",0\
^ - s\
^vJ-^ -
/-">^
V
SITE/
LOCATION/
LEAD
Indiana Toll Ro»d Service
Plaza as1*
Rolling Prairie, IN
USTPRP
Ionia Company Road
Commission Garage1'
Ionia, MI
UST lead (state)
i&D (Union 76)b
Miles, Ml
0ST lead (state)
J. Thomas Snu th Trustb
Rose Township, MI
tJST tod (State)
X
JH Baxter & Company
.Eugene, OK, x ^
State Isad (State Superftmo*-^
km CERCLA) - * s
^ \
•Johet Army Ammunitions
Plant "
33iwood,,Jl,
Federal facility -\ " x
* < *•
Joslyn MFG
Brooklyn Center, MN
CERCLA state lead
feyliwyej Ah; r^j Jfex.f
MarquktS.-lRli'^kf ^*f§k- t-^s-J
w^^M^\~^i^ti
BrScess8-! 4,--*\,\ c isAv^j- < ' ~ •
!>> -«•; :*><• ,- •: ;-;, v ^V- x~? ••
• •°'J;Vty',-i ^'.^S'4,/;-"^'
Pro^ss^y; -x-' »;>5;|t" -; 4:.
^-^V^'v^lV^r' {^ 'v^^',-v^^'
,-' v^ ^ v*1" **'* ^ -* "- ~V'X' V-v ^!c 7
^''-V -' -• *-.C.\ ^•^' '^Vo^iP'^.-
K.I. Sawyer Air Force
Base — fire training1"
Marquette, MI
CERCLA state lead
CONTACT/
PHONE
NUMBER
Steve Myers
(317) 240-8085
ComnJctor/
engineering firm
Michael B. Sleaze!
(517)373-0249
State organization
Michael B Stenzel
(517)373-0249
State organization
Michael B. Sterne}
(5*7)373-0249
State organization -
Ani)tev1n0
) . '
Basis: risk-based
Ground wajef;(2,SA6-tetracWoa)prienoI; PCP; toial PAHs)
Other  ;
Fu!l-scafeteifl6di8
06/93.-
(Predesign, . ' •-
^ N^^\ ^ 0^
Full-scale remediation has been underway since
08/89. Expected completion 08/95.
Laboratory-scale studies have been completed.
yul^i^ienle^o^^
ptedes'ignr§im^K^^
^4-' J'^-i?'-^^^'-->'< j-O, >" -J \ >,;•* ; :^j%-,i', jv^si-Vi- - A- >••",'•"
. ,t,,'< 'oi^V,: ,;,?>, *%v\;- A^.-.J™ ^ss,;JSf,C »-<.«; S---,.
Full-scale remediation is planned. Currently in
design. Pilotscale studies have been underway
since 09/93.
TREATMENT
In citu treatment, soil bioremcdiation (in situ land treatment).
Nutrient addition (urea with other nutrients). Aerobic
conditions, exogenous organisms.
In situ treatment, air sparging, bioventing. Oxygen source.
Aerobic conditions, indigenous organisms.
In situ treatment, ground-water bioremediaaon Oxygen
source, nutrient addition (type not identified). Aerobic
conditions, indigenous organisms.
ID situ treatment, ground-water bioremediatiort, soil '
bioremediatkm (in siru1and.treatment). Hydrogen peroxide,
nutrient addition (nitrogen and phosphorus) Aerobic
conditions, indigenous organisms.
Ex M (rsatment, fixed Shrt, plug flow, Aerobic condjhons,
exogenous organisms^ tx %
E^ situ treatment, sequencing batch reactor, land tre^trnent.
"- * ^ ° ^ ^ I
0 ^ ^
^ ^ ^ ^ ^
Ex situ land treatment. Nutrient addition (inorganic nitrogen
and phosphorus). Aerobic conditions, indigenous organisms.
In'jlf js treafeiSibioventtingjAefobto feaftf<»5^di|^tw£JS\
$l^§I^^Mp^Sfi^?-^!r;;
1?^v5Si%-'-:>S ^C^^^^IS?
V\fv "'V^^ ^.^ v- "" "'»"" \:^'o\^",":o°',VN s*V ^ iv-^"?N •%
Vr^';i"-\A'VY ''^"v-X.\-; "';- c^^C^^'l" Vj"7 ^~ -°'^ ';>\'^
i^ v'lf oNN;i- v^V^""'. ^"^"^^ ^\<"Vx ^^ - \ %»'v -vi-v^'\-- ^ x
In sj|« ^|^eji^biQyenttij^,AeroMc,wndinon8»"imligenott^'X
^f^rp>^f ^^^v^f | ^m^
In situ treatment, bioventing, soil bioremediation (in situ land
treatment). Oxygen source. Aerobic conditions, indigenous
organisms.
Indicates a new site.
c Indicates thauhe site has been updated.
Shading indicates ajion-CERC

-------
                FIELD APPLICATIONS  OF BIOREMEDIATION  (cont.)
REG
   SITE/
LOCATION/
   LEAD
CONTACT/
  PHONE
 NUMBER
    MEDIA/
CONTAMINANT
                                                                                           STATUS
             TREATMENT

      Kenworth Truck Company
      ChiJlicothe, OH
      CERCLA state lead
                 Kay Gossett
                 (614) 385-8501
                 State organization
             Vadose soil (TPHs, 40 mg/kg)
             Ground water (benzene, 0.005 ppm; ethylbenzene, 0.7 ppm;
             acetone, 0.25 ppm; toluene, 1 ppm; xylene, 10 ppm)
                           Full-scale remediation has been underway since
                           03/94.
In situ treatment, ground-water bioremediation. Ex situ
treatment, GAC bioreactor. Hydrogen peroxide, nutrient
addition (nitrogen, phosphorus), completely mixed flow.
Aerobic conditions, indigenous organisms.
      Kincheloe Air Force Base
      Kinross, MI ,
      CERCLA state lead
                 Michael B. Stenzei
                 (517)373-0249
                 State organization
             Soil (benzene, 24 (ig/kg; ethylbenzene, 1,500 |ig/kg; PCS,
             14 Jig/kg; hydrogen cyanide, 2,000 ug/kg; TCE, 44 |ig/kg;
             toluene, 16K (ig/kg; TPHs, 100 ug/kg; xylene, 5,600 ug/kg)
             Basis: risk-based
             Other contaminants: cyanide, lead
                            Full-scale remediation is planned. Pilot-scale
                            studies were completed 10/94. Started 05/94.
In situ treatment, bioventing. Oxygen source. Aerobic
conditions, indigenous organisms.
b Indicates a new site.
0 Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

-------
                    FIELD APPLICATIONS  OF BIOREMEDIATION (cont.)
 REG
         SITE/
      LOCATION/
         LEAD
CONTACT/
  PHONE
 NUMBER
                                     MEDIA/
                                CONTAMINANT
                                                                                                          STATUS
TREATMENT
       Lawson Oil*
       Oshtemo, MI
       UST lead (stole)
                          Michael B. Stenzel
                          (517) 373-0249
                          State organization
                Ground water (benzene, 12. ppb; toluene, 790 ppb; xylene,
                280 ppb)
                Basis: risk-based
                                                                  Full-scale remediation is being conducted.
                                                                                                                                    la situ treatment, ground-water Woremediatiou, soil
                                                                                                                                    biorcmcdiaiion (in situ land treatment), reinfection. Oxygen
                                                                                                                                    source. Aerobic conditions, indigenous organisms.
       M-21 and Kerby Road"
       Corunna, MI
       307 State Lead
                          Michael B. Stenzel
                          (517)373-0249
                          State organization
                Vadose soil (acenaphtfiene, 500 ng/kg; benzene, 24 [ig/kg;
                pbcnanllirene, 500 (tg/kg)
                Basis: risk-based
                                                                  Full-scale remediation is planned. Currently in
                                                                  design.
                                                                                                                                    Aerobic conditions, indigenous organisms.
       MacOillis and Gibbs
       Company Site
       New Brighton, MN
       CERCLA fund lead
                          Daryl Owens
                          (312)886-7089
                          Federal organization
                Ground water (PAHs; PCP)
                Basis: publicly owned treatment works prelreatment standards
                Other contaminants: arsenic, chromium
                                                                  Full-scale remediation is planned. Currently in
                                                                  design. Expected start 11/95. Pilot-scale studies
                                                                  were completed 09/89. Started 07/89.
                                                                                                                                    Ex situ treatment, fixed film, plug flow. Aerobic conditions,
                                                                                                                                    indigenous organisms.
       Mackinaw Island State Parr
       Mackinaw, MI
       307 State Lead
                          Michael B. Stenzel
                          (517)373-0249
                          State organization
                Soil (pbenanthrene, 500 |ig/kg)
                Basis' risk-based
                                                                  Fall-scale remediation is planned. Currently in
                                                                  design.
                                                                                                                                    Ex situ treatment, bioqell, Nutrient addilion (type not
                                                                                                                                    specified). Aerobic conditions, indigenous organisms.
       Marathon #2809"
       Kenrwood, MI"
       UST lead (state)
                          Michael B. Stenzel
                          (517)373-0249
                          State organization
                Ground water (benzene, 1 2ppb;ethytbenzener74ppb;
                toluene, 790 ppb; xylene, 280 ppb)            ""
                Basis; risk-based
                                                                  Full-scale remediationls being conducted.
                                                                                                                                    Anaerobic conditions, indigenous organisms
       Marathon Station-Brvines
       Kemwood, MI -
       State tead   v   •-  -
                          Bourne White-
                          (616)45,6-5071-  •
                          State organization.
               ^Saturated sofl  "
                Ground-wafer (BTEX; gasoline)
                Basis, health risk-based
                                                                  Fn!l-sc"ate jernedjatioB^as been Underway since
                                                                  01/88. Laboratory-scale and pilQt-scale studies
                                                                  have been completed.
                                                                                                                                    Bx SUM treatment, fixed film, pjog flow. Actobio condition
'Maralhon-Criinksb
Ho!land,-MI
DSTJead (state)
                                Michael B.Stenzef >
                                N{517>37.3-0249 \0x
                                State organization  ,,
                                            Soil(benzene, 24 ftgrtcg; etbylbenzene, l,50j) ftg/fcgi toluene, ,
                                                                                    "
                                                                Full-scale remediationhasbeen underway since1
                                                                            '
                                                                                                          Jn situ treafoient, ground-water biprernedtatioii, soil
                                                                                                          bioremediation (in situ land treatment). Oxygen source
                                                                            _     x   N
                                            Ground water (benzene, \£y$r, ethylbenzene, 7SQ ppb;
                                            toluene, 74 ppH xylene; 280 ppb)              ' ,  "
                                            Basis;" ask-based'1'         r    -  s -      x x
       Martin Public Schools^ ° ^
       Martin. MT   "    *,
                          MichaelB. Sfenzel
                          {517)373^249:
                              s
                Soil ^benzene, 24 pgftg; eJbyJb?Bzene, },$Q6 ftg/lfeg; toluene,
                   N                                        '
                                                                (33)95.  '-   -
                                                                                                 since
                                                  ground water (benzene, 1 .2 ppb; ethytbenzene, 74 ppb;
                                                  M*JBE, 230 ppb; toltieney 790 ppVxyteiie, 2$»pp5>)
                                                                                                                                           Aerobic Coftditi6ns,ifftdigeiiousotganisrfl$ '
   V-
^IVSarv^ft 'Windows'
WarroadI MN
       jProcess 1
       Process £ x
Brace Schaible  s,
{2I8)386VJ43Q
ftdasfry    ., ^  x
                                            Ground, water, (PCP,%3prj|)>      -     ;;
                                            Basis (soil); risk-based ° "'   ^        *  ^    ;s   *
                                            'Basis ground water): state health risk, limit •  "xV  ' *M  ^
                                            Oll^r contaminabts- Mercury is piesenf ^Fhe-quesuon of  „
                                                                                                  Fall-scale remediation is (Sanriedi Cntreatly Mug
                                                                                                  installed. Laboratory-sole stadfes have been > x   N
                                                                                                        ~E\ sito treatraent,,f3xed ftjm, completely mixed ftow.-Aerob.ic^
                                                                                                        ctfndifions, exogenous organjsnK,   ,             _
                                            examined,  t    "  "  ""           \ ^

                                            Vadose soil (PCP, 5 rag/kg)
                                            Gr9imdwater(PCP,3ppb)  "    "-'
                                            Basis (vadose soiljuisfc-based   v  \-
                                            Bas!s-{ground water), state health risk limit
                                                                Fall-scale remediafloiiis planned. Qiwently kf
                                                                                                  since 12/92.
                                                                                                          in sits treatment, sort tnotemediation (m siru land treatment). •
                                                                                                          AnaeroBic conditions, exogenous "organisms
b Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

-------
         FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
  SITE/
LOCATION/
  LEAD
CONTACT/
 PHONE
NUMBER
  MEDIA/
CONTAMINANT
                                                    STATUS
                                                                        TREATMENT
                                                                -Iff

   n
   j§l
   v,€'

b Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

-------
                    FIELD APPLICATIONS  OF BIOREMEDIATION  (cont.)
 REG
    SITE/
LOCATION/
   LEAD
CONTACT/
  PHONE
 NUMBER
    MEDIA/
CONTAMINANT
                                                                                                              STATUS
                TREATMENT
       Mobil Oil 03-NPJ*
       Union Lake, MI
       UST lead (slate)
                   Michael B. Stenzei
                   (517)373-0249
                   SMC organization
               Soil (benzene, 24 ng/kg; etbylbcnzene, 1,500 pg*g; toluene,
               1 6K [ig/kg; xytene, 4,600 ng/fcg)
               Ground water (benzene, l,2ppb;ethylbenzeneI74ppb;
               tohiene, 790 ppb; xylene, 280 ppb)
               Basis; risk-based
                                Full-scale remediation has been underway since
                                12/93. Pilot-scale studies were completed 04/93.
                                Started 04/93.
In situ treatment, air sparging, ground-water bioremediation.
soil bioremediation (in situ land treatment). Oxygen source.
Aerobic conditions, indigenous organisms.
       Moss-American
       Milwaukee, WI
       CERCLA enforcement lead
                   Ross Han
                   (312)886-4844
                   Federal organization
               Sediments
               Soil (creosote, 6.1 nig/kg)
               Basis: risk-based
               Volume: 80,000 cubic yards
               % of total vol. at site: 38%
                                Full-scale remediation is planned. Laboratory-scale
                                studies have been completed.
                                Pilot-scale studies are planned.
Ex situ treatment, slurry reactor. Nutrient addition
(phosphorus, nitrogen), batch flow. Aerobic conditions,
indigenous organisms.
       Motorola MIS Building*
       Schaumberg, IL
       UST lead (state)
                   PhiiLasala
                   (708)576-7509
                   Industry
               Vadose soil (BAP, 0,0046 mgfltg; benzo(a)anthracenet
               0.0026 mg/kg; benzo(b)fluoranthene, 0.0036 mg/fcg;
               benzo(fc)ffuoranthenei 0.0034 mg/kg)
               Basis; Illinois EPA Soil Cleanup Level
               Volume: 50 cubic yards
               %a{ total vol*at$ite;0,0l6'%
                                Full-scaleremediation is planned. Laboratory-scale
                                and pilot-scale studies are planned,
fn situ treatment, soil Moremediation On situ land treatment).
Nutrient addition (wheat germ). Aetobic conditions,
exogenous organisms.
       Murphy's11
       Wayne^MI   v
       USTJead (state)"
                   Michael B. Sfenzel
                   (517)373-0249
                   State organization
               Soil (benzene, 24 |ig/k|» ethylbenzene^ 15.6K ng*g;
               acenaphtbylene.SOO'jtg'kg; naphthalene, 5,000 jtg/kg;°
               xylene, 5,600 |ig/kg)   '-  " <>        \*"
               Ground water (benzene, I,2ppbjetl>ylbenzeuel74pspbi
                                                       790 PB(>' xylene, 280 ppb> -
                                                 Basis; risk-baled s V     ^ s    „,,
                                Fall-scale remediation has been underway since-
                                04/95. LabOHttory-scafe studies wens completed
                                12/94 StartedOI/94,  ? *x      ,  ^  *
                                Pilot-scale studies have beeu underway^sftwe
                                01/95. Expected completion 12^5. v '
In situ treatment, ground-water bioremediation, soil
bioreroedia,tton On situ land treatment). Aembw conditions,
                              °

       Jteasft College  ,v
       307 Sjate lead -„
       Process 1 -   -  <•
       Process 2
                   Michael B. Stenzei %"
                   (517)3734249' " s"
               Sea! (benzene; ethylbeazene; tet)2o{g»h4)peryieae}
                                    phenanthrene^pyrene;toln«ne|ijieae)^'    ^ ;,
                                             '       '            '"  ""
                                                             JPrtOt-fcW^ studies, were eomplet^d 1 !/93,s$tane4"~ ,
                                                             41/93^\'<  "I  '"' ^^  ^\x"-   "^
                                                                       .fiiatutr^atmenf, iiio'venttng'Oxygehsoafce; Aerobic i
                                                                       condittohs, indigenous organismsV v    -^t^-/
                                                                                                                                      Sx'sto tteatiBenttpt]er addition, of l>f»P"-5«rater-sur&eta)St. v
                                                                                                                                                                             '
                                        StenzeJ
                                             "
       New Lyme Landfill
       New Lyme, OH
       CERCLA fund lead
                   Ted Smith
                   (312) 353-6571
                   Federal organization
               Ground water (ethylbenzene, 68 u.g/L; methylene chloride,
               473 u,g/L; methylphthalate, 9.2 |lg/L)
                                Predesign.
Ex situ treatment, fixed film, rotating biological.
b Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

-------
                FIELD APPLICATIONS  OF  BIOREMEDIATION  (cont.)
 REG
   SITE/
LOCATION/
   LEAD
CONTACT/
  PHONE
 NUMBER
    MEDIA/
CONTAMINANT

STATUS
TREATMENT
      Ninth Avenue Dump Site
      Gary, IN
      CERCLA enforcement lead
                 Bernard J. Schorle
                 (312)886-4746
                 Federal organization
             Ground water (oil)
             Basis: MCLs or 10~5 cumulative carcinogenic risk
             Other contaminants: lead, nickel, salt, some other metals
                            Full-scale remediation has been underway since
                            02/92.
                     Ex situ treatment, sequencing batch reactor, completely mixed
                     flow. Aerobic conditions, exogenous organisms.


 •v^i
      Onalaska Municipal Landfill
      LaCrosse County, WI
      CERCLA fund lead
                Kevin Adler
                (312)886-7078
                Federal organization
             Vadose and saturated soil (BTEX; naphthalene; TPHs)
             Volume: 5,000 cubic yards
                            Full-scale remediation has been underway since
                            05/94. Expected completion 09/96.
                            Laboratory-scale studies were completed 03/92.
                     In situ treatment, bioventing. Aerobic conditions, indigenous
                     organisms.
      Organic Chemical
      Grandville, MI
      CERCLA fund lead
                Tom Williams
                (312)886-6157
                Federal organization
             Vadose soil (BTEX)
             Ground water (BTEX lube oil; TCE; toluene)
             Volume: 6,500,000 cubic feet
             % of total vol. at site: 100%
                            Laboratory-scale studies have been completed.
                     Bioremediation treatment not yet established.
                                                                                                                                                                 5
                                                                                                                                                                 I
b Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCI.A site.

-------
                    FIELD  APPLICATIONS  OF BIOREMEDIATION (cont.)
 REG
    SITE/
LOCATION/
   LEAD
CONTACT/
  PHONE
 NUMBER
     MEDIA/
CONTAMINANT
STATUS
TREATMENT
       Penny's Building/MFC
       National Bankb
       Murquellc, MI
       307 State Lead
                    Michael B. Sienzel
                    (517)373-0249
                    State organization
                Soil (benzene, 24 (ig/kg; benzo(a)an'thracene, 180 fig/kg;
                BAP, 180 Hgflcg; acenaphthylene, 500 Jig/kg;
                bcnzo(b)fluoranlhcne, 180 Jig/kg; chrysene, 180 }ig/kg;
                ethylbenzene, 1,500 ng/kg; phenanlhrenc, 500 [tg/kg; pyrenc,
                10K (ig/kg: xylcne, 5,600 ng/kg)
                Ground water (naphthalene, 30 ppb)
                Basis: risk-based
                                  Pull-scale remediation has been underway since
                                  12/94.
                          None—natural attenuation—passive. Anaerobic conditions,
                          indigenous organisms.
       JPetroCell1'
       Washington Courthouse, OH
       XJST lead (state)
                    Eric C.Mather
                    (513)489-6789
                    Contractor/
                    engineering firm:
                Soil (benzene, 6 fig/kg; ethylbenzene, 6 mg/kg; toluene,
                440(ig/kg; TPHs, 105 mg/kg; xylene, 28 mg/kg)
                Basis:, regulatory
                Volume: 100,000 cubic yards
                % of total vol. at site: 50%
                                                                Full-scale remediation has been completed.
                                                                          Ex situ treatment, aerated lagoon, pile. Aerobic, conditions,
                                                                          .indigenous organisms.
       Pine Bend Landfill"
       Inner Grove Heights, MN
       CERCLA state lead
                    Ken Meyer
                    (612)297-2915
                    State organization
                Ground water (acetone; benzene; vinyl chloride)
                Volume: 350,000,000 gallons
                % of total vol. at site: 30%
                                  Full-scale remediation is planned. Currently in
                                  predesign. Laboratory-scale studies are being
                                  conducted.
                                  Pilot-scale studies are planned.
                          In situ treatment, ground-water bioremediation. Nutrient
                          addition (most likely sugar-based (glucose)). Aerobic and
                          anaerobic conditions, indigenous organisms.
       Sorter Hilts Presbyfenan
       Village11
       Grand JRapds, Ml
       USTtead(sfat6)
                    Michael B,Stenzel
                    (517)373-0249
                    Slate organization
                Soil (benzene, 34 Hgte etbylbenzene, 1,500 fig/Kg, toluene, -
                16K{j.g/kg, xylene, 5,600 jig/kg)   ^            «  -
                Ground water (benzene,! 2 ppb; xylene, 300 ppb)  ,  ,
                Basts;"jiisk.-basedx
                                  Full-scale remedjajionJias been underway since
                                  01/94.              "
                          Aerobjc conditions, indigenous organisms, v
       Prilyiart*?1*
      ^Buchanan, MI -
       UST lead (state)
                    MtCBaelB.Stenzel
                    .(517)373-0249  ^
                    State organization
                Ground water (benzene 11 ppb, etbylbenzene, 7
                naphthalene, 250 ppb; TCE, 2 2ppb, toluene, 790 ppb;
                xylene, 280 ppb)    v          » \            "
                Basis'tisk-based      -   l
                                  Foil scafe reraedtSfion has been underway since
                                  04/95 Laboratory-scale studies have been
                                  underway since 01/95. - x       s
                                  Pilot-scale studies are planned, x   -.
                          In situ treatment, ground-water bioretnediation, recircuJaSrtg
                          leach ierf Bx situ treatment, sltwy reactor Oxygen source,
                          nutrient aMtion, (type not yet Mennfied), completely mixed
                          flow f robtc Conditions'indigenous oigamsms.
       Say's Landscaping/Nursery'
       Walled Lake, Ml
                    <.S17) 373^249
                    Sttte organization
                Soil (naphthalene, 5,005 Jtg/kg> xyfene, 5,000 }ig&g) '
                Basts* risk-based        (  \
                Vofeme 610 cube yards  x  *  v^v  NO   "
                •% of total vol at site: lixm  ^        ^^ ^  ^
                                  Fttll-scatereiaedtafloa has been Mderway since
                                  03/95. Laboratory-seals and pitot-scale studies
                                  have been completed,
                          Ex situ treatment, pile Oxygen source, nutrient addition. 
-------
                  FIELD APPLICATIONS  OF BIOREMEDIATION  (cont.)
 REG
   SITE/
LOCATION/
   LEAD
CONTACT/
  PHONE
 NUMBER
    MEDIA/
CONTAMINANT
                                                                                                  STATUS
               TREATMENT
       Reilly Tar & Chemical
       Company
       St. Louis Park, MN
       CERCLA enforcement lead
                 Daryl Owens
                 (312)886-7089
                 Federal organization
             Vadose soil (2-fluorobiphenyl; acenaphthylene;
             acenaphthene; anthracene; benzo(a)anlhracene;
             benzo(b)fluoranthene; BAP; benzo(k)fluoranthene;
             benzo(g,h,i)perylene; chrysene; dibenzo(a,h)anlhracene;
             fluoranthene; fluorene; indeno(l,2,3-cd)pyrene; naphthalene;
             PAHs; phenanthrene; pyrene)
             Volume: 2,000 cubic yards
             % of total vol. at site: .2%
                             Full-scale remediation is planned. Pilot-scale
                             studies have been underway since 11/92. Expected
                             completion 11/95.
 In situ treatment, bioventing. Nutrient addition. Aerobic
 conditions, indigenous organisms.
       Ritari Post and Pole"
       Sebeba, MN
       CERCLA state lead
                 DaleTrippler
                 (612)296-7746
                 State organization
             Vadose soil (PCP, 40 mg/kg)
             Basis: risk-based
             Other contaminants: dioxins
             Volume: 5,000 cubic yards
             % of total vol. at site: 100%
                             Full-scale remediation is planned. Currently in
                             predesign. Laboratory-scale studies have been
                             completed.
                             Pilot-scale studies are planned.     »
 Ex situ land treatment. Aerobic conditions, indigenous
 organisms.
      Seymour Recycling
      Seymour, IN
      GERCLA enforcement lead
                 Jeff Gore
                 (312)886-6552
                 Federal organization
             Soil (DCE; TCE; vinyl chloride)
             Volume: 111,000 cubic yards
                            Full-scale remediation was completed 09/90.
In situ treatment, soil bioremediation (in situ land treatment).
Nutrient addition (potassium, nitrogen, phosphorus, sulfur).
Aerobic conditions, indigenous organisms.
b Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

-------
      FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
V
V
V
V
- V
V
X
\
^

SITE/
LOCATION/
LEAD
Sheboygan River and Harbor
Sheboygan, WI
CERCLA enforcement lead
Process 1
Process 2
Shell"
Grand Rapids, MI
UST lead (state)
Shell Service Station"
Rockford, MI
UST lead (state)
Shell— Allen Road15
Southgate, MI
VST lead {state) - "" '
Lincoln Park, MI
UST lead {state)' „
X X - v.
-Shell— Eureka Road" ^ x 1
Sotitfegate, MI , \
USTlesd (state) v x ""
X- - ^ V v~ •>""
x \ X »
Shejl — HH> and Grand River"
"Brighton, MI \ >N x
USTleadXstate} ^ , x~
CONTACT/
PHONE
NUMBER
Bonnie Eleder
(312)886-4855
Federal organization
MichaelB. Stenzel.
(517)373-0249
State organization
MichaelB Slenzel
(517)373-0249
State organization
Mtchael B. Stenzet
(517)373-0249
State organization , /
MichaelB. Stenzel -
<5f?) 373-0249
State organization
»,x vx
\
Michael's StenzsP
$5 J?) ;J ?3*0i24i?
St^iC 6rii&|H2atl6ll <. »>
s "• ~ ^
- \ x r -
X \. ) '
Basis: risk-based^ , ' s v ' ° s
V«lome 8?0 cooTo yards X ' % x
%-of total vol. atsaej 100% x x^ " v v
v k\x- N- s
^oil (benzsne^^t ^g/fe etbylbaswne, 1360 flg/kgv toluene^
Vol«BKK4,008cabjfeyai^ N xV , , x
5&of total "wLat^te. 100% x x°""
x -> N x, xx <, x x ^^
«j[,500 M*g, ^168^4600 }tg%gj , '
Groand-water fbeazeae, 1.2 Bpb; ethylbenzene, 74 m*;
(olaene,?99ippb,*xylene,'280ppi>} ^ , x
BasisjasK-based > "" v
STATUS
Full-scale bioremediation is not planned.
Laboratory-scale and pilot-scale studies have been
completed.
Predesign.
Full-scale remediationnasbeenunderway since
01/95.
Full-scale remedialionhas been underway since
01/95 Laboratory-scale studies were completed
06/94,
Full-scale remediation has been underway since
12794 Laboratory-scale studies were completed
06/94, Started 01/94.
^ \ ^
* *" ** >o ^ x
Full-scaie tsmedtadon has been underway since
12/94 Laboratory-sale studies were completed N
'a/94 Started 01/94
' x X x
^ "" 0 X
^ ^ x s
Full-scale remediaaonha^been underway since
^04/94 Laboratory-scale studies' were completed
1-2/94, Started 01/94 , ; ,
Pilot-scale studies have been underway since
01#"x Jv
Pilot-scale studies have feen underway since
01/92. , \, x " - - -
x v xX l '
- v "- ^ , x
TREATMENT
Ex. situ treatment, confined treatment facility (lank). Aerobic
and anaerobic conditions, indigenous organisms.
In situ treatment, sediment bioremediation.
Anaerobic conditions, indigenous organisms.
In situ treatment, ground-water bioremediauo-n, soil
bioremediatton (in situ land treatment) Nutrient addition
(,036% yeast & sugar solution). Aerobic conditions,
indigenous organisms.
In situ treatment, ground-water bioremedialion, soil
biotemedianon (in situ land treatment) Oxygen source,
.nutrient addioftn (sugar, yea^ and vitamins). Aerobic <
conditions indigenous organisms ' x v
X ^
1 ^ x > v ^ , °
to sira treatment, ground«-water btoremedtation, s^tl
bifwemediatton 0n s«u land treatment)^ periodic, ground-water
recovery <3xygeisaur.ee> nutrient addition Aerpbic
conditions, tadigenpus organisms x
X- ^N I *, N^
X ^ ^ ^
In situ treatment^ swlbioremediatfoB (m sita land tteattoent). x
Nutrient addition (yeast andsugar). Aaaerobic conditions^ <
thdigenous organisms s - -
v X N ^
X ^ X X s
s " \X ^
3 ^ *- ^ X
< * *• ^
injsitu treaflnent, soil biorewediation {in s$tu land tteatment), ,
Oxygeh source. Aerobic conditions, indigenous organisms
\ '^ * ^ X " N
b Indicates a new site.
0 Indicates that the site has been updated.
                                                                  I

-------
                FIELD  APPLICATIONS OF BIOREMEDIATION  (cont.)
 REG
   SITE/
LOCATION/
   LEAD
CONTACT/
  PHONE
 NUMBER
    MEDIA/
CONTAMINANT
                                                                                        STATUS
             TREATMENT
      St. Louis River
      Interlake/Duluth Tar Site
      Duluth, MN
      CERCLA state lead
               Ann Bidwell
               (612)296-7827
               State organization
            Soil (PAHs, 0.08 mg/kg)
            Basis: risk-based
            Other contaminants: occasional low-level cyanide
            Volume: 3,300 cubic yards
                          Full-scale remediation is planned. Currently in
                          predesign. Laboratory-scale studies are being
                          conducted.
In situ treatment, bioventing, soil bioremediation (in situ land
treatment). Ex situ land treatment. Nutrient addition (nutrients
undetermined at this point). Aerobic conditions, indigenous
organisms.
      Stoughton City Landfill
      Stoughton, WI
      CERCLA fund lead
               Beth Reiner
               (312)886-4783
               Federal organization
            Ground water (dichlorodifluoromethane; THF;
            trichlorofluoromethane)
            Basis: risk-based
            Other contaminants: iron, magnesium, manganese
                          Full-scale remediation is planned. Currently in
                          design. Expected start 06/97. Laboratory-scale
                          studies are planned.
In situ treatment, ground-water bioremediation.
 Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

-------
                      FIELD APPLICATIONS OF BIOREMEDIATION  (cont.)
   REG
    SITE/
LOCATION/
    LEAD
CONTACT/
  PHONE
 NUMBER
                                    MEDIA/
                               CONTAMINANT
                                                                                                                  STATUS
                TREATMENT
          Sunstrand Sauer Company
          La Salic, IL
          UST lead (slate)
                    Craig Steinheimer
                    (217)782-6761
                    Stale organization
                Vadose soil (1,1,1-TCA, 0.2 rag/kg; U-DCA, 0.005 ragflcg;
                benzene, 0.005 mg/kg; TCE, 0.005 nig/kg; tetrachloroethetie,
                0.005 mg/kg; toluene, 1  mg/kg)
                Volume: 5,000 cubic yards
                % of total vol. at site: 100%
                                                                 Full-scale remediation has been underway since
                                                                 07/93.
Ex situ land treatment. Aerobic conditions, exogenous
organisms.
          Swanson's Amoco11
          Grand Rapids, MI
          UST lead (state)
          Process 1

          Process 2
                    Michael B. Stenzel
                    (517)373-0249
                    State organization
                Ground water (benzene, 1.2 ppb; ethylbenzene, 74 ppb;
                naphthalene, 250 ppb; toluene, 790 ppb; xylene, 280 ppb)
                                      Soil (benzene, 24 fig/kg; naphthalene, 5,000 (ig/kg; xylene,
                                      5,600 jig/kg)
                                      Basis: risfe-based
                                                                 Full-scale remediation is planned. Currently in
                                                                 design. Expected start 1 0/95. Pilot-scale studies
                                                                 were completed 03/95. StartedOl/94.
                                                               Full-scale remediation is planned. Currently in
                                                               design. Expected start 10/95, Pilot-scale studies
                                                               were completed 03/95. Started 01/94.
In situ treatment, air sparging. Oxygen source. Aerobic
conditions, indigenous organisms.
                                                                                                        In situ treatment, bioveming. Oxygen source. Aerobic
                                                                                                        conditions, indigenous organisms.
CJ1
N>
          Swiss Village
          Brodhead, WE
          DST lead (state)
                    DinqTsoris
                    (608)275-3301
                    State organization
                Soil (benzene, 5.5 ng/L; ethylbenzene, 2,900 ug/L; toluene,
                1,500 Hg/U xylene, 4,100 p;g/L)
                Ground water (benzene, 0.5 ng/L; ethylbenzene, 140 ftg4.;
                toluene, §8.^ ngVL-xyfene, 124 pglL)     ^ ^      X-
                Basis (soil): interiro soil cieapup standards
                Basis (grotod water): kdminisirati.ve code  ;
                Volume: 2^00 cubic yards    ,       "
                % of fatal *Df; at site:
                                                                 Full-scale remediation has been underway since
                                                                 03/92.
In situ treatment, ground-water bioremediation. Ex situ
treatment, air stnpper. Oxygen source, nutrient addition
(phosphorus). Aerobic conditions, indigenous organisms.
         ,Taber;&Ottenwess1>x-.
          WaIker,,MF   ;-
          6ST lead (state)" >
                    Michael BlStenzel, „
                    (517)373J0249>
                    State organization
                          s
                Ground water-(benzene", 1.2 ppb")
                Basis: risk-based  -     , ^"
                                                                                                       Anaerobic conditions, indigenous organisms. ,   '„'
                                                                 OW4.   ' -
          The UNO-YEN Company*'
          Tj3mont,1L  N   , ' s
          RCRANtead (federal) "X ;
,Todd Grate}  " N"
(312)886-5909
Federal organization.
                                                                                     Fall-seale remediadon. was completed 05/93,
                                     249,
                    State organization  "
                Sp|! (benzene: efcylbenzene; tolifcne>xyfene)
                                                                 Full-scale remedJaaonlias been underway sjuce
                                                                 OIKS. Lab'oratork-scafe studies were completed
                                                                                          "
                                                     Basis, nsk-'bised  v %
                                                                                                        N                x
                                                                                     P(Nlot"-scaJe studies ww^ coropleted !3/94. Started
                                                                                                   '
                                                                                                       biorerBediation (in situ land tre9tmefll).sOxygen source,
                                                                                                       antrienf Addition Xerobic condtadns, indigenous organisms,^
          flymoitt&.Ml
          -.USTTead (state)
                    •Michaels, Stenzel
                    (517j"373-0243
                    State organization^ 2
                foil (bei»en.e;24 figflrg; ethylbenzeae, Js,500 (jg/kgj'x
                 "             "  '  •>  "x °-° "  "  -"  ^
                                                                                                    Fall-scale rsmedia6on has been underway since-
                           ^
                GroundxWater (benzene, 1 2 ppb; ethylbenzene, 74 r»pb;
                KtTBE> 230ppH foluene, 790 ppb; iyteae, 280 pp'b)  s
                       '        "                " "   S
                                                                                                         Ittsitutteatment, gtound-waterbiotemedtatioj), soil s
                                                                                                         biojeraediatioB 
-------
         FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
y
,v *
> *"
,v>'r
,V ;
^r?
\ *
"\V"I
y
v- .
v
vX
V '
<<
£
"V-
0:
SITE/
LOCATION/
LEAD
ffMai sasse1" , •. 'V ,* • \
.WafcvMf ' . ;• %
USiytead (state) - • >*• -,*
Total #26S>^ ; ' - V"-;x
,.% Johns', Ml', " V - "'
USTJea'a^state) „
'x-.x ~'v' \ ' v A4> '•
Totai/Ro-Oads* - , f ? ""• "°-
Climax^M! f" s _v
USftead(s!ate) , •* ,
\ > x '
TiteIeWvCarWash V" ," ;
Mun4etem>lEvi , \
^ite lead not supplied < °
X $ -°° " - N> '
" • ^ ^ -?N \^
Union 7
facility -c x * \>
Kal'atnkoo, Mfc v_ °« - " ^" °'
'R£RA lead (stata) '- ^
NO"' •. j ^ "" 'C "
Van Manen'Oit Company11 - •• "
G^utfejjydVMI-^,' , \,, , s ,
.USTheMl (state) 'V Ni ^ '
s\ % ' )* " '' r <
< ' i * \ j " f
Versendaal Ijncoto-Mejyury1*
Hbi1atod,MI- \v, •*'•';
OST tead (state)' ^> •» ^ _'
Village of Meats', ^ .•• « •*
%(jraund»ya(erconfaminatioa* ;>
MearS,MI " ° "«',"',
5^7 State Lead " -^ '-^'.
- - '• %x--^r- N J,
CONTACT/
PHONE
NUMBER
^Michael B.Stenzel. t'
;(517>373.0249>~ " ,l
SHteprgaiit?aUi» '*
X >^ X " N S
Michael B^Stenzel x
,<517) 373-0249 (Jf ;'
State organization
"Michael BsSfenzel.' '
-(5h) 373-0^49 s N-
Statefcrganization ^s
^ ^ s\ *
Christopher ^ < •>
Kohmiaiin -."„•. ,'
i[217)782-676l ;" ^--.
sState organfzatt0a.o -
n JN c'\> "n
Michael Bc'Sienzfil
,(517)^73-0249 _
Siate organization 
Federal organization -,
> ''-, => '•":
MtchaeJ'B.^teTizk s
(51?) 3^J024r' -,
State organizaflon ••
::">^:\>"<>;
^ ^ j ^ j
Michael B.Stenz^) "
(517/373-0249 ""' -
S»te'"orgaHizatioa s
'v 1 ^ ^
Michael B!|l(6pz6l
t(5l7)373-'024?s^r
< State oj|aniz8(ifm> N '
^ s \ t" ,\
^ ' * >v I"
MEDIA/
CONTAMINANT
^ 0^n2ene,24Vgfl«g;xe%yiben2ene, l,5W fig/kg; tbliea^.
16K|ig*g;xylene, 5,600 pg/kg) ^,V^ ^ "-"
Ground water^beu^etie itippbsetbyltfetHiene.Wppb;,
toluene, 790 ppb;^ytette;2kOppb) - _s ,\ ov."->,
Basis; rtsfc-based. „ < -
«,"' V ! ,, %"' ^N ' N ' - >
Soil £benzet%24 ftgfltg; eftylbenzene; 1,500 pg/k£; xyienes,
sioOjlg/kg) ' ;-^\ >', "•"'">/' < . » • -' ^ ' " "
"OroWtMater, (benzene^! ,2 ppb; etnylb?nzene; WppbT '
^MTBE,2it3ppb;tolueneK790ppb;xytene,28'Oppb) -'; ' ^
Basis- nsk-baied t . > s •> v^ -A s- c *.''•<•
-, ~- ^^ ^°~-" x N ' . * x
Ground wafer (beBzene, hlppb^ethylbenzeaeAppb-^ - '
MTBE,<230 ppb; »luxene,;790 ppb; xy teat) 280 ppb) s x,-.° ' -
Basis' risk-based ^ - ' v '"•' Nj ^x-
)^"N ^~)S \n-i '^vJS^x'' ^^n"s\
^Soil(beteene,25'p:g%BTBX^16.0i5'mg*S>-.'^ "-," '•
JBasts: baselinfeobjeotiye^ • 1 x\ ,' ^> »x ^^" s- /
Voluma: 2,000 cubic yard/ s N ^ < t.^ '
'%oftotalvol,at«ite:100% " : * <« -"v\
s '-* ' "• ^ " \"~ ""• ' \ -\ ^N ^X VX > N* \
Ground water '(benzene,-! ,2 ppbl ethylfonzene, 74^>pt>; ;^ " N
Wuenes7s9pppb;vxylepe,286ppb). . .^^c.^ ^••*
•BasKi'tisk-bastedv '- '- , s s K" ~ >, ' "^
° - . „ ,- - x "^ ~- * s- ^ < ^ * * - > *
Groxind.\vat«f'(benze!tte, !-.2ppb $(hylbenzene,74pjib; \ ,
JyllBS, 230 ppb, tolije^e, 750 ppb; xylene,s 280*pp*ri)
-Ba5ts;stis!c-based< N - - ^ '""/.'/ >x ' ' x
Oro&dVater (t-butanots benizene; dtlorbbenzene} 1,2-DCA;
;cb,l^foOT,eth.yJbenzfenelTHF;tWuette;%l<;ne> l; v<" > -
>$oil'(b«m^nf'24p;g/fetxj'law;s,600|igft:g> . -;
ground •water (benzene, 1 ,2 ppt{, acenaphtbyleSeCSS ppb; , "
•-iluorene, MO pjb; fljph«fjatene,°t50 "pplg phenanthiene;, "•
25ppb;xylene,280ppb)'" " •'."- ' v>, 's
iBasB-qfea^X^V *„„, ' •' ( .^
sGround,water"'(ethylb'enzene,7'4 ppbj naphtnalene,'25b ppb; »
^ytene,280gpb.) > « ] > ;"\ -o ._ , _ i '^^ - y" J" "
Bask risk-based ss ^^, ' t ^, ' °> - -1 ^ > T  ^_
xylehe) . ' , • ' '-,-,,• ^ ^ ^
Basis; nste-basetT "" -; . x • ^ \ \>, ^
Other contaminants; metals (1 300 pptnleM) •; * ' %
STATUS
Fall-scale remediation.IiaS'been underway siijce " -
0»4S. . ^ xX- "- •< ' ' "\~\ ~~
A>' ' N "•' *' \
sFall«scale remediation is being conducted, - N -^ '„ -
Laboratory-scale studies we pjalwed.'-'s" /
*X ' ' •" „ ><^('> " ^A«J
' Mkeale remediation is planned^Currently in v x x
.predesign Lahomtt>ry^scate8nd piipt-seafc studies" "••
at« planned, ' --1 "< N s " *x ^ ^ "
- " "' ^"',<*< xJ . ' " " 7^ "" *•
'<• N <>s\ ^ „"•"",•• A ° „ ^
'Fail-scale bforemedlation is not planned^ x ^ \ -
> **? ' " x,\ , ° *•"" ' ~ -
Fall-scale remediation is being conducted, \ ' **
Pilot-scale studies are planned. ' AXS , » ' >
-V* ^' ° , " , ^j1 ',\ ~ ^ t-'<." '
Fuil-scate retnediartoaisas been underway ^ince ^ s
01/94."" ^ ^" '•„ "• ^ ," ' ""f x
* ' , * s- - " \'l <-* ^^« -"' ^ < "
Fall-scale remediation isj>lanned. Ci^enfly in s ^_
prettesfgwl'ildt-sca!evstu((iesiissve,been«tnderway''s
sincdm/S7 v ' ' ^ "- ^ " ^ ^ ","*"..'-
,-,,„ - yt „- , s| vj." ^ "^ \ -, ;
Ml-^caferemeSiati^n isv]jlanned.'CutteBtly in.-^ >*"•'
predesfgn,, '•' ^ °. : I ^ \""<^
, < s^*\s . > ^'= £ * N> ^ > - >
°^s / S S, " ^ • N s\' ^ S
; *'. *' %^ ~ > x x >4\ 3
Pull-seafe rentediation tiaxbeen" underway since •
01^5. ^ f,"v ' x - ° X\V 4"^'' "s
« N °* ^ ' l^ '' ' '"• •> '„
\ „ v< s.>'..*t .">. J ,,,.. • .. l ." ., «
Full-scale i'emediajion'h^s been und&Tyay^sirtce ,
05^ Pilot-scale stadies are planned^' s
^ 'XC'"" ^ si ^v' -v>
- N- •>,'''"
TREATMENT
'fiisito treatment ground-water btoremedtation, soil^ s >,. - '
bioremediadon (ui'sita land.treatment) Aerobic conditions, ' ^
^ndi'genqusargan.tsnK.^ , ^ •.,*<•, '' , ,\ '
'SlsiatHeatntent,grQUnd-waterbiQremediation,"so11, < - „
biorenjetjiatton (in situ Jajadtreatjioeat), Oxygen source, - "
rainientaaditibiiOit^dgenyphospnoftis/potassinm' 36.6:6) ^,,
Aptobjc conditions, indigenous orgaM^tfas, ^J" - -\^ -
•^"^ x ->f i. ' ;.\ s ; ^ *v<1 ', ,s>- •• .
tesiai treatment, ground-'water btoretnediation.'pTjtse systeni"" -
•OxygenSoun;e,ntitrientaddit!Qfl,Aerobife<;Ondition^, -
intfigenoas organisms.- '' ; '< O ,ol^' ^* \" X >
vv -C "• * '"v"
In sttutreaanen^ soil bioremedlation (in situ land treatment), v
Nutfient addition (amniWinn chloride, potassium phosphate; ^
sodiunithexametaphosphate^ Aerobic condition^ jndigenous "•
ofganisins.-" ^^ ! °° \ ' ° • ; , • *• • "<\ "" ; s < ^;
,n,r\ 	 ;„„„., ,,;|7 	 -,;;„„' ^,}a,,,i.,'n 	 , 	 6l ^..liisu 	 .tulr;,,,™,,:, 	
In. sita'treatment," ground-water bioremedtadori; soil ' s * !
bioreiiwdiation^insjw tend treatment)' "' ' , s "*. ^ ^ ,
In situ treahnem.ground-^'ater bioremfidiation" Oxygen
source. Aerobic conditions, indigenous oVgamsms,; ^^ ^ f."
s"x^*^ >x*,,^ S>
E^sft^Veatment, fixed film, non-ae«ted.lagoottj completely"
jnijsed^ovs-, Aerqbic conditions, indigeDousoiganisitis^ s'
' --"Xi }> '' s5 -\ \ ;""*-x< "^r*. ^'V'v",
In siat treWment, 'ground-water btoreBiedtation soil x *^\ ^*
bjorernediation (uj slt» land teattnent)". Oxygen-source „' ,
AerbbicconditianS,)tfdigeno\is\)rganismss v '_ • ^\t ^ t '
% - XA5'f,' ? , ^ >v ; x', r * ^s\ 5, •. i %
s Aerobic conditions, inofgenousorganisnas,- " ; N ll^^
\ . txN " I ,>. x - ^ \ ' \ ' \ , >v. ' i
, ~ ' <«;,">>* " -:-1-' ,o "••.*
>|h$itatre^tnfentJgfotind"-^erbiOKiMid!8flpn, spil' - ,., 4,«
',bioreniediation(insitt),iand1reatnient) BxAsifu (reatmeiit,' '^'
^ftoidi^d bed. Hydrogen peroxide, oxygen source^ nutrient * v
^ addition (nitrogen and phosphorus), baKhflow. Aerobte
cftndittpns^jndigenqa^organfsros^ V '- -' '••'" - /^
CJ1
w
  Indicates a new site. -
 c Indicates that the site has been updated.
 Shading indicates a non-CERCLA site.

-------
       FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
V
V
V
V
N.
y '
ty.
V>.'
V
V
*. \
SITE/
LOCATION/
LEAD
Waibridge Mangier"
Livonia, Ml
UST lead (slate)
Warehouse Properties'"
Detroit, Ml
UST lead (slate)
Warner Lambert
Company— Parke-Davis
Holland, Ml
RCRA lead (federal)
Welsh Oil— Wro 2 Truck
Plaza* * t >,t,
pentonHarbof.M < „
UST teatf (state) ,t <
WescftOil*2i* x
FrattpartjMJ " ,
UST lead (sSafe) "
(,<- f , > %
WescepiUfS" ;
iCaJajnazoo, }5tl ,, x
USTSsKNjsiate} x
n 1 1 1 1 L ^
WeseoGilSlB1' N . x
North, Mtrekegon, MI „"'
UST lead (state) x
WeseoQilSjS?1'
afoskeglSttjYH.
OST lead (state) "
i ^
CONTACT/
PHONE
NUMBER
Michael B. Slenzel
(517)373-0249
Stale organization
Michael B. Stenzel
(517)373-0249
State organization.
ShanSutkw
(312)886-6151
Federal organization
Michael B. Stenzel
(517)373-0249
State organlzatipit
Michael B.»er«»l
,(5I7-)3B-0249<
Sf8|i?; organization N
\ f
* * > \- N *
^ i ^\ ^
Mfchaal'B- Steazet
(517)373-024^
State 0rga.mza&cm
V N
\ ^
MtcliaetB.Sfenzet
^517)373-0245 ,
'State efganoafioa.
^
Mtchae! ft. Stenzel'
 <
i *^v \\ ^ i \ ^ ^ v x
- v* v ^ >. ^14,
Soil pjeBzeBe,:24tigte«5>ylb«n^ne» 1300 jtg*g,)VtfBB, v
1 600 flgfltfetsiltiei^^lSKjig^^ylene, 5,600 wgftg) >
Orouad^-watef (benzene, 1& gpb; ethylbenzene, 74 ppfas
toluene, 790 ppTs; xyfenej^^ppb) x< *^
Basis nsfe-baseci »x *^ ^ <
x ' \ \, *• *
§oi| |be^zene, 24 f^/kg; eftylbajiene, l,50^|ig/kg, toluene,^
16^ jigftgj xylene, 3,6Qp^g*g| °v ^x ^- t-T
Oroiwd v^ata" flienwrte, 1 2 ppb; etbylberaenei 74ppl>, x
tolwne, 790 ppi>- xylene, ZSO ppb)
Basis nsk-based .. ' - * . »
" ^ s, l *-,
Gtem&sxssc. {benzene, 1,2 aigflisMrBE, 230 vagfL}* "• "
paraUuan-e,74n»g/S.> ^ ^ x •• \
Ba«s.ask-l>asAl *• x . * v \ *
Volroe, 2,40&i?tibif; yards- •> T " N
•%"&«tofai4VQl ittsrte;40b ^ s . ' °
S«i Cbertzene^ragflkg; etnylbensseneracenapliihylerte, xX ,
5tX>Tng/fcg,benzo(a)anlhracenB,330itng/fcgeh!ysene, •>
330ingifkg, lead, 4Wiag/Jcgv toluena; xylene)^
Oroiaidu^ter (beteeae, 3 & mg&; ethylbenzenfc, 74 m^L,
toluene, 790 tng/L;xytene,.^80irig/L) ^ ^ *
Baornsk-based s "^ '"'"<,
Otfeercontarairiants lead x - x x
STATUS
Full-scale remediation is planned. Laboratory-scale
studies were completed 12/94. Started 12/94.
Full-scale remediation has been underway since
01/93. Laboratory-scale studies were completed
06/91. Started01/9l.
Pilot-scale studies were completed 06/92, Started
12/91,
Mboratory-scateand pilot-scale studies are
planned
Rin-scateternediauon m planned Currently being
installed, ^ * ., v« t *
Fwll-scafeiemediationis being eoodttcted.. t
Pifot-stfale sttKites.were eompleted 12/94 Started
Ql$4, >/" •> ' t-
\0 *> ^ "> as>heea«iid^iva¥StitCfe
03/93^Htot-8cale studies are piannW ^
' X * S
^\ -t <-
" \" "*«
-\> \>
Fall»^catereniedJS!ion'liasi*eaanaer*ay|ince <•
01/9S Hftt-scatesaidres have been underway "> °
sas 5>ges underway since
•06/94 Puot-scftte siudtSS have been 4lttdfir*ay \
since 12/93 x
< > f: <. < *"
x * ^ ^ °\
" \ V
X s v, <,^^ o V ^ -
\, *.
TREATMENT
In situ treatment, ground-water bioremediation, soil
bioremediation (in silu land treatment). Oxygen source.
Aerobic conditions, indigenous organisms.
Ex situ treatment, pile. Aerobic conditions, indigenous
organisms.
Ex siru treatment, fixed film. Aeiohw conditions
Anaerobic conditions, Indigenous organisms. •,
* >v *" * > x «.' ^
ln,stw wtmefit, s«I bio«wediato» S» site ISnd t&at^iero). - "
Sx-s,*". "' -, ^ ^ N V ^ . * X
ffisttu treatrneat^airspargingjsoil Jjforemediafton- (mainland.
treahriemt). ^x^gea source Aerobic candttfons; indigenoos
«gajtisws f ' ' sXx-- \, "" v ' ^ v '^*v
4 Wv v 1 -, *
?^ °^o ^ ^ C
Anaerobic condiSons, instgenoas- prgawsms ov
^ v* ^ ^ » ''
1 x
In siro treatrnenr, softj^orernsidiatieit ^n, stta tad treatroeat)
Ex srai'ireatmerft, shirr}' reactor Oxygen seur&, nutnent
atMaoiCeomrjlefelyiiHxecfilQW Aerobic conditions, 'v
«Kiig«ttQilsars&ni$ai$ - * > „ <
, " " - \
* s " ^ "" -S
, ^ ^ , "v- ' „
k Indicates a new site.
c Indicates that the site has been updated.

-------
                   FIELD APPLICATIONS  OF  BIOREMEDIATION  (cont.)
 REG
   SITE/
LOCATION/
   LEAD
CONTACT/
  PHONE
 NUMBER
    MEDIA/
CONTAMINANT
STATUS
                                                                                                                                                   TREATMENT
       West K&L Avenue Landfill
       Kalamazoo, MI
       CERCLA enforcement lead
       Fixed-film reactor
       In situ ground-water
       bioremediation
                   Dan Cozza
                   (312)886-7252
                   Federal organization
               Ground water (acetone, 700 ppb; benzene, 1 ppb; 1,2-DCA,
               0.4 ppb; TCE; 1,1-DCA, 700 ppb; ethylbenzene, 30 ppb;
               toluene, 40 ppb; trans-l,2-DCE, 100 ppb; vinyl chloride,
               0.02 ppb; xylene, 20 ppb)
               Other contaminants: metals may be present

               Ground water (1,1-DCA, 700 ppb; 1,2-DCA, 0.4 ppb;
               acetone, 700 ppb; benzene, I ppb; ethylbenzene, 30 ppb;
               TCE; toluene, 40 ppb; trans-1,2-DCE, 100 ppb; vinyl
               chloride, 0.02 ppb; xylene, 20 ppb)
               Other contaminants: landfill contents contain
               non-biodegradable materials
                                Full-scale remediation is planned. Currently in
                                predesign. Laboratory-scale and pilot-scale studies
                                are being conducted.
                                                                                 Full-scale remediation is planned. Expected start
                                                                                 12/98. Laboratory-scale studies are being
                                                                                 conducted.
                                                                                 Pilot-scale studies are planned. Expected
                                                                                 completion 06/97.
                         Ex situ treatment, fixed film, batch flow. Aerobic conditions.
                                                                      In situ treatment, ground-water bioremediation. Aerobic and
                                                                      anaerobic conditions, indigenous organisms.
 VY;,
 ~W
                                         l^fCS""^
  VI
       Atchinson—ATSS Santa Fe
       Lake
       Santa Fe, NM
       CERCLA enforcement lead
                   Ky Nichols
                   (214)655-6783
                   Federal organization
               Sediments (diesel, l,000mg/kg)
               Soil (hydrocarbons, 1,000 mg/kg)
               Basis: state guideline (California)
               Other contaminants: chlorides
               Volume: 28,000 cubic yards
               % of total vol. at site: 100%
                               Full-scale remediation has been underway since
                               07/92. Pilot-scale studies have been completed.
                        In situ treatment, soil bioremediation (in situ land treatment).
                        Ex situ land treatment. Nutrient addition. Aerobic conditions,
                        indigenous organisms.
  VI
       Chennault Air Force Base
       Lake Charles, LA
       CERCLA fund lead
                   Larry Poindexter
                   (504) 862-2937
                   Federal organization
               Soil (benzene, 10 mg/kg; JP-4 jet fuel, 250 mg/kg)
               Basis: state cleanup level goal
               Volume: 27,000 cubic yards
               % of total vol. at site: 100%
                               Full-scale remediation is planned. Currently in
                               predesign. Remediation expected completion
                               12/95. Pilot-scale studies are planned.
                        Ex situ land treatment. Aerobic conditions, indigenous
                        organisms.
b Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.
                                                                                                                                                                                        1

-------
        FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
VI
VI
VI
VI~
\
^ 1
'VI
VI
SITE/
LOCATION/
LEAD
Dow Chemical
Company — Louisiana Division
Ptequemine, LA
RCRA lead (stale)
Dyess Air Force Baseb
Abilene, TX
Federal facility
ST-40
ST-41
French Limited
Crosby, TX
CERCLA enforcement lead
FSAX-1 and 3, Air Force <,
Plant 4b
JFon Worth, TX
Federal facility s
TPSA-1
FSA-3 "• v. "' "
*• •?- ^ *- *
Hudson Refining Company
Cashing; OK: « \
RCRA lead (federal}
x o (. o
\
^KettyArrForceBaSe
San, Antonio, TX • *
Federal facility
CONTACT/
PHONE
NUMBER
Madeline Murphy
(504)765-0585
State organization
Marty Faile
(210)536-4331
Federal organization
Judith Black
(214)655-6739
Federal organization
Lt Co) ROSS Milter.
(FSA-I)
(210)536-4331
Federal organization
Ronnie Crossjanc!
(214)655-6480
federal organization
Kenny Johnson
aiO}S25-mi ""
Federal organization*
MEDIA/
CONTAMINANT
Ground water (1,1,1-TCA; 1,1-DCA; 1,1-DCE; 1,2-DCA;
chlorocthanc)
Volume: 90,000 cubic yards
% of total vol. at site: less than 1 %
Soil (benzene; ethylbenzene; toluene; TRPHs; xylene)
Basis: state regulations
Soil (benzene; ethylbenzene; toluene; TRPHs; xylene)
Basis; state regulations
Volume; 600 cubic yards
% of total vol. at site: 10096
Sludge (BAP, 9 ppm; benzene, 14 ppm; PCBs, 23 ppm;
VOCs, 43 ppm)
Ground water (hazardous contaminants)
Basis: MCLs (risk-based)
Sort (benzene; ethyftenzene, toluene, TRPHs, xylene) N »
^ > X "*
\^ >
s v i = ^
^ v \ v "• ^ ^ ^
"- > ^ \
* \
Soil (benzene, 0 5 rng/fcg; ethylbenzene, 0 5 mg/kg; toluSneK
0 5 mg4g,TRPfi3l xylene, 0,5 ing/kg) "• <
^ \> \ ^ "
^ ' •• \ s ^
\ v *
Vadose soil ^AP, 200 Jig/kg; benzo(a)arttliracene, v
200 (»g*g, cjirysene 200 jig/kg, total oil and greases
960 mg/fcgi total PAHs^IS rng/kg) \ s
Volame; 145,000 ouinc yards
% of total vol '318116:40% ! ( ° \ ,- ^
Vadose soil (]&enzetie^TBXj«W,i!thyIberr|er«; PCB;
DCE?TCE;tdlBeae, vinyl ciitori(te;xyleae) " x^° *
'-'* v*N " *- '•^
^ N V ' * ^ v' ^
STATUS
Full-scale bioreinodiation is not planned.
Laboratory-scale studies were completed 12/90.
Pilot-scale studies were completed I2/9Z
Full-scale remediation is planned. Pilot-scale
studies were completed 12/94. Started 12/93.
Full-scale remediation is planned. Pilot-scale
studies were completed 12/94. Started 12/93.
j
Predesign.
Fttli-seafe wmedtafi^nis^lanned, Pilot-scale -
'itadtes'liave been underlay iSince 1 1/93
^ " - ^ *> 0
"\ 'NK v *
! X
Full-seale remediation is planned PHot-scale s •
swdtesrjwe been UBiJerWa^since 03/94 x " ^ f
F«l)-sca'fe«nie(8iit'on,lia& been underway siaoex <,
'Ot/86. -J X/ \ -. V- .
^
J 5 \ ^
v ,x\ N t
Fall-soaJe reawdiatiqttlias been underway .siaee
-02®3 N x-."
TREATMENT
In situ treatment, ground-water bioremediation. Nutrient
addition (acetate, glucose, orcthanol proposed). Anaerobic
conditions, indigenous organisms.
In situ treatment, bioventing. Oxygen source. Aerobic
conditions, indigenous organisms.
tasltu treatment, bioventing. Oxygen source. Aerobic
conditions, indigenous organisms.
In situ treatment, air sparging, pure oxygen dissolution
system. Oxygen source, nutrient addition (for soil, water, and
sediments). Aerobic conditions, indigenous organisms.
Jn siw treamia«; bioventtn^ Aerobic *ojidins, indigenous
orgaaisins; ( ' * , " ^^,
td,siru treatrnent? bioventmg> Aerobic, conditions, indigenous
organisms. - , " °^
X \ ^ N ^
!n $«» treatment ami bnasmediatior* 
-------
               FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
  REG
      SITE/
   LOCATION/.
      LEAD
CONTACT/
 PHONE
 NUMBER
   MEDIA/
CONTAMINANT
STATUS
                                                                                                           TREATMENT
Ul
XI
                       " Fidera! drgaiiizkffon0.
                       ''•
                                                                    - Wi-s&e fibifeaJjafion }s^not^plaaiS,d:v,4 % ijcj-, '^i
    VI
MacMillan Oil Refinery11
Norphlet, AR
CERCLA fund lead
                        Charles Fisher
                        (214)655-2224
                        Federal organization
           Sediments (phenanthrene, 34 mg/kg)
           Basis: landban restrictions
           Volume: 300 cubic yards
           % of total vol. at site: less than !%
                       Full-scale remediation has been underway since
                       08/94. Laboratory-scale studies were completed
                       12/92. Started 10/92.
                       Pilot-scale studies were completed 05/93. Started
                       03/93.
                  Ex situ land treatment. Aerobic conditions, indigenous
                  organisms.
  b Indicates a new site.
  c Indicates that the site has been updated.
  Shading indicates a non-CERCLA site.

-------
       FIELD APPLICATIONS OF BIOREMEDIATION (cont.)






















Cn
00























REG

VI


VI
























VI





VI




VI ^


SITE/
LOCATION/
LEAD
North Cavalcade Street
Houston, TX
CERCLA state lead
Oklahoma Refining Company
Superfund Site
Cyril, OK
CERCLA state lead
Process I


Process 2




Process 3












Old Inger
Darrow, LA
CERCLA state lead



PAB Oil and Chemical
Services, Inc."
Vermilion Parish, LA
CERCLA enforcement lead

Randolph Air Force. Basek
Rrodolplt-AFB TX "
Federal facility

CONTACT/
PHONE
NUMBER
Glenn Celerier, P.E.
(214)655-8523
Federal organization
Terry Andrews
(405)271-7157
Stale organization






















Paul Sieminski
(214) 655-8503
Federal organization



Jamie VanBuskirk
(214) 665-6767
Federal organization


SantTaffinder
(2-10)536-4331 <
Federal organization


CONTAMINANT

Vadose and saturated soil (benzene; PAHs, 1 mg/kg)


Ground water (1,2-DCA, 0.005 mg/L; benzene, 0.005 mg/L;
ethylbenzene, 0.7 mg/L; 2-melhylnaphthalene, 0.15 mg/L;
2-melhylphenol, 1 .8 mg/L; 2,4-dimethy!phenoI, 0.73 mg/L;
4-methylphenol, 1.8 mg/L; naphthalene, 0.15 mg/L; phenol,
22 mg/L; toluene, 1 mg/L; xylene, 10 mg/L)
Other contaminants: arsenic, barium, beryllium, cadmium,
chromium, copper, lead, nickel, zinc
Sediments (benzene, 0.2 mg/kg; 2-methylphenoI, 12 mg/kg;
4-methylphenol, 14 mg/kg; BAP, 0.33 mg/kg;
benzo(a)anthracene, 4.1 mg/kg)
Volume: 43,334 cubic yards
% of total vol. at site: 30%
Sediments (BAP, 0.33 mg/kg; benzo(a)anthracene,
4.1 mg/kg; benzo(b)fluoranthene, 0.69 mg/kg;
2-methyInaphthalene, 12 mg/kg; benzo(k)fluoranthene,
13 mg/kg; benzene, 0.2 mg/kg; chrysene, 46 mg/kg;
dibenzo(a,h)anthracene, 0.33 mg/kg; indeno(l,2,3-cd)pyrene,
3.2 mg/kg; naphthalene, 79 mg/kg; phenamhrene,
1,080 mg/kg)
Vadose soil (2,4-dimethylphenol, 66 mg/kg; 2-methylphenoi,
12 mg/kg; 4-methylphenol; phenol)
Other contaminants: high concentrations of arsenic, lead,
chromium, and other heavy metals
Volume (sediments): 46.6k cubic yards
Volume (vadose soil): 2.6k cubic yards
Sludge (hydrocarbons)
Soil (petroleum)
Other contaminants: metals may remain, but they are not
expected to be above health-based levels.
Volume: 100,000 cubic yards
% of total vol. at site: 70%
Sludge (PAHs, 3 ppm)
Basis: regional policy
Other contaminants: heavy metals, barium, arsenic
Volume: 29,000 cubic yards
% of total vol. at site: 100%
Soil (benzene, efhylbenzene; toidenei^TRPffc; xylene)
Bass stateiregolstwns •* "' ,<;« N
' ' ' ' \ <• ' ' '


STATUS

Full-scale remediation is planned. Currently in
design. Pilot-scale studies were completed 10/92.
Started 06792.
Full-scale remediation is planned. Currently in
design. Expected start 03/97. Laboratory-scale
studies have been underway since 05/94.




Full-scale remediation is planned. Currently in
design. Expected start 03/96. Laboratory-scale
studies have been underway since 04/94.


Full-scale remediation is planned. Currently in
design. Expected start 03/97. Laboratory-scale
studies have been underway since 04/94.
Pilot-scale studies are planned.









Full-scale remediation has been underway since
10/94. Laboratory-scale studies have been
completed.
Pilot-scale studies were completed 1 1/86. Started
10/85.

Full-scale remediation is planned. Laboratory-scale
and pilot-scale studies are planned.



FaD scalft^mediadonlsplatHied.sPilot-scaIe •>
-studies wetfc qornpl«ed03/94 Started 03^3,, "
^ X > ' * N , \ /*


TREATMENT

Ex situ land treatment. Aerobic conditions, indigenous
organisms.

In situ treatment, ground-water bioremediation. Nutrient
addition (to be determined during design). Indigenous
organisms.




In situ treatment, sediment bioremediation, soil
bioremediation (in situ land treatment). Aerobic conditions,
indigenous organisms.


Ex situ land treatment. Aerobic conditions, indigenous
organisms.











Ex situ land treatment. Aerobic conditions, indigenous
organisms.




Bioremediation treatment not yet established.




to situ treatment, bfovenrtng Aerobicconditions, indigenous,
organisms, ^ so - „
N
•> ' * ° "
                                                                   Q.
                                                                   5°
                                                                   3:
11 Indicates a new site.

c Indicates that the site has been updated.

-------
                  FIELD  APPLICATIONS OF  BIOREMEDIATION  (cont.)
 REG
   SITE/
LOCATION/
   LEAD
CONTACT/
  PHONE
 NUMBER
    MEDIA/
CONTAMINANT
STATUS
TREATMENT
  VI
       Sheridan Disposal Services
       Hempstead, TX
       CERCLA enforcement lead
                  Gary Baumgarten
                  (214) 655-6749
                  Federal organization
              Sludge (benzene; ethylbenzene; PCBs, 50 ppm; phenol;
              toluene)
              Soil (benzene; ethylbenzene; PCBs, 50 mg/kg; phenol;
              toluene)
              Surface water (benzene; ethylbenzene; PCBs; phenol;
              toluene)
              Volume: 40,000 cubic yards
              % of total vol. at site: 100%
                              Full-scale remediation is planned. Currently in
                              predesign. Laboratory-scale studies have been
                              completed.
                              Pilot-scale studies were completed 12/91. Started
                              04/91.
                       Ex situ treatment, slurry reactor, completely mixed flow.
                       Aerobic conditions.
      Conservation Chemical
      Kansas City, MO
      •CERCLA enforcement lead
                  Steve Auchterlonie
                  (913)551-7778
                  Federal organization
              Ground water (semivolatiles; phenols, 1 ppb; VOCs, 5 ug/L)
              Basis: EPA or Missouri drinking water standards
              Other contaminants: cyanide complexes, nickel, zinc
              Volume: 200 gallons per minute
              % of total vol. at site: 100%
                              Full-scale remediation has been underway since
                              01/90. Laboratory-scale studies were completed
                              01/89.
                       Ex situ treatment, fixed film, completely mixed flow. Aerobic
                       conditions, exogenous organisms.
  VII
      Fairfield Coal & Gas
      Fairfield, IA
      CERCLA enforcement lead
                  Bruce Morrison
                  (913)551-7755
                  Federal organization
              Saturated soil (benzene, 241 mg/kg; ethylbenzene; PAHs,
              500 mg/kg; toluene; xylene)
              Ground water (benzene, 1 ppb; ethylbenzene; PAHs, 200 ppt;
              toluene; xylene)
                              Full-scale bioremediation is not planned.
                              Laboratory-scale studies have been completed.
                              Pilot-scale studies were completed 01/93. Started
                              12/91.
                       In situ treatment, ground-water bioremediation, injection and
                       extraction wells. Hydrogen peroxide, nutrient addition (water,
                       (nitrate)). Aerobic conditions, indigenous organisms.
b Indicates a new site.         •>  ,
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

-------
        FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
vir



VII
„•
VII
vn
x-
-
SITE/
LOCATION/
LEAD
Ofliitt Air Force Base"
OffuttAFB.NB
Federal facility
Building 30
Building 406
LPDSite
POL Area
Qfnm Air Force Base— JP-4
Bellew,NE
Federal facility
Bioventing '•> " _
Insitaground-waterv° ' >
biorernediation
Owens-Corning11
Kansas City, KS
CERCLA state lead, RCRA
lead (state) (Site lead is not
known with certainty because
cleanup was completed several
years ago.)
-Park Oty
Park City, KS "
RCRAlea
<405) 436-8532
Bate! organization:
1 3-
MEDIA/
CONTAMINANT
Soil (benzene; ethylbenzene; toluene; TRPHs; jcylene)
Basis: state regulations
Soil (benzene; ethylbenzene; toluene; TRPHs; xylene)
Basts: state regulations
Soil (benzene; ethylfaenzene; toluene; TRPHs; xylene)
Basis; state regulations
Soil (benzene; ethylbenzene, toluene, TRPHs; xylene)
Basis state regulations
Vadose soil (benzene, 10 mg/fcg; ethylbenzene, 10 rag/kg;
TPHs, 10 mg/kg; xylene, 10 mg/fcg)
Baasr risk-based „
QBier contaminants; ar$etiic, barium1, lead, zinc, v
Volume' 1 1,000 cubic yards v N x
% of total vol. at site; 55%^- * >v *' •
Ground water (VOCs^ ° • N -->x
jBasts; risk-based * % Jx ^ ""VN
% of total vol at site: 69^ ^ ' , ~ "" - - "
Soil (formaldehyde, 1 mg/kg)
Volume: 300 cubic yards
% of total vol. at site: 100%
Ground wtter Ibenzene^ w-xyletie; ethyfbenzenef Ojxylene",
p-xyleae; toluene) ,^ " t •, , * ^ ,
Basis i federal MC3bsv x^ x -,
Other contaminants' long chata (high molecular teight)
Volume: 22^800 cubic meters ^ \
% of total vol. at site: 50SI " v^"_ , \-
STATUS
Full-scale remediation is planned. Pilot-scale
studies have been underway since 05/93.
Full-scale remediation is planned. Pita-scale
studies have been underway since 10/93.
Full-scale remediation is planned. Pilot-scale
studies have been underway since 08/93.
Full-scale remediation is planned. Pilot-scale
studies have been underway since 05/93
Fall-scale remediationhas been underway since
10/93. Expected completion 10/95 PiIoHcate „ >
•studies have been underway since 08/92,
^ > X.
Full-scale remediation is planned. Laboratory-scale \
state were- completed 08/94, Started^ 08/93
Pilot-scale studies are planned Expected •- x-x
rorapfetipn 08/95. "'^ •• s \ »
Full-scale remediation was completed 07/92.
FuH-$$ate remediation Msb^n underlay -smci, * •- <
05793. Laboratory-scalesttidies were completed*^.
OW3, Started OU92 <• „ . N "
x \ ^
^ \ "*
\, ^ SN\NJ v
TREATMENT
In situ treatment, bioventing. Aerobic conditions, indigenous
organisms.
In situ treatment, bioventing. Aerobic conditions, indigenous
organisms.
In situ treatment, bioventing. Aerobic conditions, indigenous:
organisms.
In situ treatment, biovenung Aerobic conditions, indigenous
organisms.
In situ treatment, biovsnting Oxygen source. Aerobic
conditions, indigenous organisms
In situ treatment^ ground-water' bioismediation Hydrogen
peroxide. 'Aerobic conditions, indigenous organisms v
Ex situ treatment, pile. Aerobic conditions, indigenous
organisms.
In situ t^tment, grouifaVwatSr ijioremedtatiort, sediment
tbio»mediatiott; Nutrient addition (mftjitels added as an \
electron receptor^. Aerobic and anaerobic coifdttioBS,^
inoligenousorgamsms v
^ &^ X ^ V v^ x
'^ \ < . ""
Ox
O
 b Indicates a new site.
 0 Indicates that the site has been updated.

-------
                    FIELD  AFFL1UATlUrNS  UF  mUJfcWMUJJlATlUlN  (cont.)
 REG
         SITE/
     LOCATION/
         LEAD
CONTACT/
  PHONE
 NUMBER
                   MEDIA/
               CONTAMINANT
                                                                                                                   STATUS
                                                          TREATMENT
  VII
       Scott Lumber
       Alton, MO
       CERCLA fund lead
                          Bruce Morrison
                          (913)236-3881
                          Federal organization
                Vadose soil (acenaphthylene; fluorene; anthracene;
                benzo(b)fluoranthene; benzo(a)anthracene; BAP, 14 mg/kg;
                benzo(g,h,i)perylene; benzo(k)fluoranthene; chrysene;
                dibenzo(a,h)anthracene;fluoranthene;
                indeno(l,2,3-cd)pyrene; naphthalene; PAHs, 500 mg/kg;
                phenanthrene; pyrene)
                Volume: 15,900 tons
                % of total vol. at site: 90%
                                                 Full-scale remediation was completed 11/91.
                                                 Started 06/90.
                                          Ex situ land treatment. Aerobic conditions, indigenous
                                          organisms.
  VII
       Vogel Paint & Wax
       Maurice, IA
       CERCLA state lead
                          Jack Generaux
                          (913)551-7690
                          State organization
                Soil (benzene; ethylbenzene; MEK; toluene; xylene)
                Other contaminants: lead, mercury
                Volume: 40,000 cubic yards
                % of total vol. at site: 100%
                                                 Full-scale remediation has been underway since
                                                 10/91.
                                          Ex situ land treatment. Aerobic conditions, indigenous
                                          organisms.
  VIII
Broderick Wood Products Siteb
Adams County, CO
CERCLA fund lead
Bioventing


Ex situ land treatment
                                 Tom Simpkin
                                 (303)771-0900
                                 Contractor/
                                 engineering firm
Vadose and saturated soil (PAHs; PCP)
Basis: mass removal
Other contaminants: dioxins
Volume: 133,000 cubic yards
% of total vol. at site: 44%

Vadose soil (PAHs, 20 mg/kg; PCP, 40 mg/kg)
Basis: land disposal restrictions (LDRs)
Other contaminants: heavy metals, dioxins
Volume: 81,000 cubic yards
% of total vol. at site: 27%
Full-scale remediation is planned. Currently in
design. Pilot-scale studies were completed 09/93.
Started 07/93.
                                                                                                    Full-scale remediation has been underway since
                                                                                                    08/94. Pilot-scale studies were completed 11/93.
                                                                                                    Started 07/93.
                                                                                                           In situ treatment, bioventing. Nutrient addition (not yet
                                                                                                           established), air. Aerobic conditions, indigenous organisms.
                                                                                                           Ex situ land treatment. Aerobic conditions, indigenous
                                                                                                           organisms.
  VIII
       Burlington Northern Tie Plant
       Somers, MT
       CERCLA enforcement lead
                          Jim Harris
                          (406)449-5414
                          Federal organization
                Soil (PAHs, 36 mg/kg)
                Ground water (PAHs, 0.03 ng/L)
                Basis (soil): risk-based
                Basis (ground water): water quality criteria
                Other contaminants: zinc
                Volume: 60,000 cubic yards
                % of total vol. at site: 75%
                                                 Full-scale remediation has been underway since
                                                 09/93. Laboratory-scale studies have been
                                                 completed.
                                          In situ treatment, ground-water bioremediation, in situ
                                          saturated soil bioremediation. Ex situ land treatment. Oxygen
                                          source, nutrient addition (nitrogen, phosphorus). Aerobic
                                          conditions, indigenous organisms.
 Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

-------
       FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
VIII
VIII
vtn
VIII
••vyt
-
^ °5
N <
VIII
'Jljft
~\

*vra
SITE/
LOCATION/
LEAD
Conoco Landfarm
Billings, MT
RCRA. lead (state)
Ellsworth Air Force Base1"
Ellsworth AFB.SD
Federal facility
Ellsworth Air Force Baseb
Ellsworth AFB, SD
Federal facility
Area D Fuel Storage
Building 102 Fuel Station
\ ^ * ^
Exxon Landfaan *
BjJltpgsTMT v
RCRA leadjtstate) •- <*
^ , ^ V ^
F,,E. Warren Air Force Base"
FE, Warren AEB.WY ^ * >
federal facibjy v <
Pit? TrS1mni> Pif 1
SplllSttel
> \
Geraldine Airport
Geraldine,MT
CERCLA state lead
Glasgow Former Air Force' x
Base
Glasgow WTT
F$dera! facility
GlenrockOilSeep"^ v
Converse, WY
OPA- Oil Pollution Removal
Action.
CONTACT/
PHONE
NUMBER
MarkMohorcich
(406)444-1430
State organization
Dell Peterson
(605)385-2675
Federal organization
SamTafSnder
(210)5364331
Federal organization
< \* s
MarkMohokieh
{4Q6)J44-!430^ -
State organization,
V- X V \ °
.terry Hansetr
(210} 536-4331 >
"Federai-orgaBizatioB ,
< \
Aimee Reynolds
(406)444-1420
State organization
Steven Ott
(402)221-7670;^ „
~,
Steven Way
(303)294-7080
Federal organizatfoa
MEDIA/
CONTAMINANT
Sludge (K048 organics; K051 organics)
Soil (petroleum)
Basis: practical quwtiiation limit
Other contaminants: K048 metals and K051 metals
Volume: 15 acres
% of total vol. at site: 100%
Soil
Water (jet fuel; solvents)
Basis (soil): risk-based/sfate requirements
Basis (water): risk-based/ARARs
Soil (benzene, 0 OOS mg/kg; ethylbenzene, o 7 rog/kg;
toluene, I mg/kg, TRPHs; xylerte, 10 mg/kg)
Basis' state regulations
Soil (benzene, 0 005 tng/kg; etbylbetwene, 0,7 mg/kg;
tohiene, 1 mg/kg; TJRPHs; xytene, 10 mg/kg)
Btists. state regulations 0
\ \ ^
Sludge (KQ49 organksj K050 organies; K051 organicsX^
Bastst practical qtiaor|t8tjon iirrat ,
Other ojtUaintBantS' K049 rnefals, K050 raetals, ar«I K051 ,^ ^
nJetals " • ' . ^ x "« x ' :
Volume, 45,000 tons xt ° N
,\^- \ ^ N< N H «,
SoH'(BTBX,XRPiHs> \ J^ . , ' "
Basis, state tegalations. N < •• '^
^ i *
V t^ ^ , ^ V s ^
^ < '
< \ \
Soil (beiteene; ethylbf nzene, toIueneTTRlfiilS; xyJeue) ^ <
Basis; state regalattotiSv ' \ ^
"x ' "" >N "i \ " ^ ;
Vadose soil (2,4-D; aldrin; chlordane; 4,4'-DDE; 4,4'-DDD;
4,4'-DDT;-p-BHC; dieldrin; endrin; toxaphene)
Vadose^soil (BtBJC 10 wg/fcg, TPHs, 100 mg&gX
Basis DIRPaandaid' x » x ' •
% f)f total Mil at Sii^l.OG?!' - , -, * \"
x ^ \
Basts' sheen on surface tyater tvv „ ^
Volume.^OQO cubic yards x 'x ,- N ' :
\ S-- ^ x *\ ^ * o ^
STATUS
Full-scale remediation bus been underway since
01 /73. Pilot-scale studies have been completed.
Pilot-scale studies have been underway since
06^4. : . - :
-•.,-.-
Full-scale remediation is planned Pilotscale
studies have been underway since 08/93.
FuU-scalejremediationJs planned. Pilot-scale
studies have been underway since 08/93
' X ' ^ *
Full-scale »e'roedtation has been, underway since ^
01/^0. ftlbt-scale srsidies have been corapteted. "
, , •- - > '
i *\
Fall-scflegteroetiiaiion Js planned. Pitot^oale s
smdiesareiierngeondttcjed . x •;
\0 ^ "-NVv •
Full-scale remediation is planned ^tot-seals
studies are being conducted s *
Full-scale bioremediation is not planned.
FuU-scaHe-remeAariorus plaonei
\ Si
** -* ' ^
S 0 ^ ^
Full-scale temedJatton is plaaned Currently In
predesigo - s s »
* X X " S <
x <• V , '
TREATMENT
Ex situ land treatment. Aerobic conditions, indigenous
organisms.
In situ treatment, air sparging, bioventing. Oxygen source.
Aerobic conditions, indigenous organisms.
In situ treatment,- biovewmg Aerobic conditions, indigenous
organisms
In situ treatment, btoventing. Aerobic condmons, indigenous
organisms.
3 ^
Ex situ land treatment Aerobic condiflons, indigenous
Organisms, ^ .> ^ "• * -,
" < " - " v^ .,- ' -.'
"• x\' " N »
In situ treatment, koventing "Aerooio condidons, tadigenciBs
organisms , t >, <• . ^^
•in sitntreatmenr, bioventing. Aerobw condiiiohs indigeBoas
oiganisms^ -.x^ ^^ x *"x«t''°
^ ^ s v
s \ t-^t-
Aerobic and anaerobic conditions, indigenous organisms.
Ex siro land treatment Aerobic concisions, indigenous
or^aaisws x" ^ "\ _ \
^ ^ ^
in siw treatment, soil bioremediatton (in situ (and treatment).
Oxygen source, nutneqt addition {nitrogen, phospboms.)
Aerobic conditions, inrfigemws organisms. ^ ^.
Indicates a new site.
c Indicates that the site has been updated.

-------
        FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
              CONTACT/
               PHONE
              NUMBER
  MEDIA/
CONTAMINANT
b Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.
                                                                                3'

                                                                                f

-------
                    FIELD  APPLICATIONS  OF BIOREMEDIATION (cont.)
 REG
    SITE/
LOCATION/
   LEAD
CONTACT/
  PHONE
 NUMBER
     MEDIA/
CONTAMINANT
                                                                                                                 STATUS
                                                        TREATMENT
  VIII
        Idaho Pole Company
        Bozeman, MT
        CERCLA state lead
        Ex situ land treatment

        In situ ground-water
        bioremediation
                    Jim Harris
                    (406)449-5720
                    Stale organization
                Vadose soil (fluoranthene; BAP; benzo(b)fluoranlhene;
                anthracene; benzo(g,h,i)perylene; benzo(a)anthracene;
                benzo(k)fluoranthenc; chrysene; indeno(l,2,3-cd)pyrene;
                PCP, 48 rag/kg; phenanlhrene; pyrene)

                Ground water (2,4,6-lrichlorophenol; fluoranlhene; chrysene,
                0.2 fig/L; benzo(b)fluoranthene, 0.2 ng/L;
                benzo(a)anthracene, 0.1 ng/L; anthracene, 0.3 (ig/L; BAP,
                0.2 ug/L; benzo(k)fluoranthene, 0.2 ug/L; fliuorene;
                naphthalene; PCP, 1 [ig/L; phenanthrene; pyrene, 0.4 ng/L)
                                 Full-scale remediation is planned. Currently in
                                 prcdesign.
                                                                                     Full-scale remediation is planned. Currently in
                                                                                     predesign. Laboratory-scale and pilot-scale studies
                                                                                     are planned. Expected completion 03/96.
                                        Ex situ land treatment. Nutrient addition (nitrogen and
                                        phosphorus). Aerobic conditions, indigenous organisms.
                                                                          In situ treatment, ground-water bioremediation. Oxygen
                                                                          source, nutrient addition (nitrogen and phosphorus). Aerobic
                                                                          conditions, indigenous organisms.
  VIII
       Joliet Weed Control District
       Joliet, MT
       CERCLA state lead
                    Aimee Reynolds
                    (406)444-1420
                    State organization
                Vadose soil (2,4-D; dicamba; MCPA)
                Other contaminants: arsenic
Full-scale bioremediation is not planned.
Pilot-scale studies were completed 09/91.
                                                                          In situ treatment, soil bioremedialion (in situ land treatment).
                                                                          Aerobic and anaerobic conditions, indigenous organisms.
  VIII
        Lake County Weed Control
        Ronan, MT
        CERCLA state lead
                    Aimee Reynolds
                    (406)444-1420
                    State organization
                Vadose soil (2,4-D; atrazine; 4,4'-DDD; 4,4'-DDE;
                4,4'-DDT; aldrin; 0-BHC; Y-BHC; chlordane; dicamba;
                dieldrin; endrin; Far-go; methoxychlor; Tordon)
                                 Full-scale bioremediation is not planned.
                                        Aerobic and anaerobic conditions, indigenous organisms.
  VIII
       Libby Ground-Water Site
       Libby, MT
       CERCLA enforcement lead
       Bioreactor
       Ex situ land treatment
       In situ ground-water
       bioremediation
                    Jim Harris
                    (406)449-5415
                    Federal organization
                Ground water (PAHs, 40 |ig/L; PCP, 1.05 mg/L)
                                                   Soil (PAHs, 88 mg/kg; PCP, 37 mg/kg)
                                                   Volume: 45,000 cubic yards
                                                   % of total vol. at site: 100%
                                      Ground water (PAHs, 40ppt; PCP, 1.05 ppm)
                                      Basis: risk-based
                                      Other contaminants: NAPLs
                                      Volume: 200,000,000 gallons
                                      % of total vol. at site: 100%
                                 Full-scale remediation has been underway since
                                 06/89. Laboratory-scale studies have been
                                 completed.
                                 Pilot-scale studies were completed 01/91. Started
                                 01/89.

                                 Full-scale remediation has been underway since
                                 06/89. Laboratory-scale studies were completed
                                 01/88. Started 01/86.
                                 Pilot-scale studies were completed 01/88. Started
                                 01/87.

                                 Full-scale remediation is being conducted.
                                 Pilot-scale studies were completed 01/89. Started
                                 01/87.
                                        Ex situ treatment, fixed film, plug flow. Aerobic conditions,
                                        indigenous organisms.
                                                                                                                              Ex situ land treatment. Aerobic conditions, indigenous
                                                                                                                              organisms.
                                                                                                       In situ treatment, ground-water bioremediation. Oxygen
                                                                                                       source, nutrient addition (potassium tripolyphosphate,
                                                                                                       ammonium chloride). Aerobic conditions, indigenous
                                                                                                       organisms.
  VIII
       Miles City Airport
       Miles City, MT
       CERCLA state lead
                    Aimee Reynolds
                    (406)444-1420
                    State organization
               Vadose soil (2,4-D; aldrin; chlordane; a-BHC; 4,4'-DDE;
               4,4'-DDD; 4,4'-DDT; p-BHC; Y-BHC; atrazine; dieldrin;
               Far-go; methoxychlor; parathion-e; Tordon)
               Saturated soil (endrin)
                                 Full-scale bioremediation is not planned.
                                        Aerobic and anaerobic conditions, indigenous organisms.
b Indicates a new site.
c Indicates that the site has been updated.

-------
                                      AFJf JL1UA'11UINS  UJb  mUJHJ£MJp,racess.. Hydtogen^eftgidd, n¥tjie,nl.a«! SitMJatnJnsmflnvr
  VIII
       Richey Airport
       Richey, MT
       CERCLA state lead
                   Aimee Reynolds
                   (406)444-1420
                   State organization
               Vadose soil (2,4-D; aldrin; dicamba; chlordane; a-BHC;
               4,4'-DDE; 4,4'-DDD; 4,4'-DDT; P-BHC; y-BHC; atrazine;
               dicldrin; endrin; Far-go; methoxychlor, parathion-e;
               parathion-m; Tordon)
               Volume: 15 gallons
                                Full-scale bioremediation is not planned.
                                Laboratory-scale studies have been completed.
                                Pilot-scale studies were completed 08/93. Started
                                01/93.
                         Ex situ treatment, reactor (type not chosen). Aerobic and
                         anaerobic conditions, indigenous organisms.
       TJpjiy. J^6ugf|!ti|urse^l!,>:--:2 :?i

      ^S^^W|i>'fi"%5'"^1
      .•l'>v---:"-.»-Xrr-^-;-^-*j
 Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.
                                                                                                                                                                                            a'
                                                                                                                                                                                            f
                                                                                                                                                                                            I

-------
      FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
VDI
j ,
VIII
VHI
t
^
~
IX
\
SITE/
LOCATION/
LEAD
Union Pacific Railroad Tie
Treatment Plant
JUramie,wy
RCRA lead (federal)
Utah Power and
Light/American Barrel'
Salt Lake City, UT
CERCLA enforcement lead
West I^ramie LUST
Remediation Project11
taramie,wy
USf lead (state)
Process I
process 2
N N "'
\ X ^
Angelas Sheet Metat \
U»s Armies,, cX"
l)ST lead {state}
N ^ "^
S^8J^4p£»&&C& v ^ !<,
s, -a. ^ O
N's \ \
\ v ^ s
CONTACT/
PHONE
NUMBER
Felix Flecrtas
(303)253-1524
Federal organization
David Ostrander
(303)293-1530
Federal organization
Kana J. Grant
(307)777-5447
State organization
S>
t, t, <*
SSSnf '
*^**':\ -
Barry Mutaaa\
.(714)^1-7112
_&Snsu1tant !>x
\ ^
MEDIA/
CONTAMINANT
Soil ' ' ',. :
Crwind water (creosote; PAHs; PCP)
Vadose soil (benzene)
Basis: source to ground-water cleanup
Volume: 570 cubic yards
	 v 	 - 	
Vadose soil (TPH5. 25 pg*g)
Ground water (benzene, 5 {igh-; tPHs, 5 (ig/L)
Basis (vadose soil), risk-based ,
Basis (ground water): MCLs - 'v
Volume (vadose soil}1 JSQX cubic yards >
Volume(gro«ma water) 340kcul>5e yards ^ „*
Vadoso sort (TPHs, 25 |lg*g) \ ^ :
Ground water f^rPHs, 5 pg/L) •• <
"•Basts (vsdose soil); rjSk-based "". ' ^s
•-Basts (grtiuad waferj MCl^ xX "" ^ * -.-
Vo)ttn»(\;adosesoilXsSQkcabic,s^aafe "^ ^'
^V^I&roe (groiin^ wat^f)ti 20Jf ^Mbio yards t *
V^dose-soif^ar^BOgeijs, ^l^in^/kg) ^ ^ >~~<
v ^ \ : c
Ortfaad wate^aemoae, l. uiethyfeite
STATUS
Full-scale bioremediaiion is not planned. *
La,ooratory-scale and pilot-scale studies have been
completed.
Full-scale remediation is planned. Currently in
predesign.
Full-scale remediation is planned' Currently in
design. Laboratory-scafesfttdies were completed
08/93. Started 05/93,
Foll-sosfe'iemedtstton is planned. Cujrently in
design Laboratory-scale studies were completed
08/93. St»atfe.d 05/93. '° t.,/^
J^u!l'ScSerernedlatii>nisplaTine(i.CmTentlyin ^ •
s "^ " V <
v > ^
04)90 "x\ - °'^> " , "> ^;
TREATMENT
In situ treatment, ground-water biorernediation, sedirnwC*
bioremediaiion, soil biorcmcdiarion (in situ land treatment).
Ex situ treatment, sequencing batch reactor, land treatment.
Hydrogen peroxide, nutrient addition (nitrogea:phosphorus). ,'
Aerobic conditions, indigenous organisms. ~
In situ treatment, bioventing. Indigenous organisms.
IB situ treatment, air sparging. Aerobic conditions, indigenous
organisms.
4i» s!tji"treatrr»w, two-phase vacuun) *xtraction,T«suUiRg irt
p'assrve'Sioventing, Aerobic «BnditiOBS, indigenous organisms.
Ibt situ treatment, ex situ on-sife bioremediaUon Aeroritc ^ .
cOj«S
-------
JL1 M.MlJjLJJLf  ZTL.JL JL  JL/JLV^-Tl. JL JLV/1! kJ  V7JL'
                                                                                                                              J. J.Vj'l
                                 CONTACT/
                                  PHONE
                                 NUMBER
                                                                                            "^n;w»^i>a4w^^nAA«^£H^>^l3-^^,«i^^H ei£a*&"'t.-"•

                                                                                                                                                                                        &
                                                                                                                                                                                        I
b Indicates a new site.
c Indicates that the site has been updated:"   ;
Shading indicates a non-CERCLA'site. ""

-------
      FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
IX
EC
* x
^N M
tx
Bf
*x "
i*
.
Wi!htj!,CA / \ \ -
California JiegMal *#ater * <
Quality Control Board
N ^ "*" f *>
BKit.iandfill
W«$tcWa,pA ' \
RCRA lea^fedetalf < ->
^ O ^ v
" ' % " X ^ s% >
°° ^ ^
Building 14?, Afr Force ,'
Kant 42"
JPalOT)ate,CA °
Federal facility^
xCALtfcANS " " "
Lafeeport^CA
dST lead (state)
CampPendleton
Building 13115
Camp Pendleton, CA
Federal facility
CONTACT/
PHONE
NUMBER
SamTaffmdcr
(210)536-4331
Federal organization
Centus Owen
(408)727-3400 A x
Industry
\
^ X
V N >
^ \\fl ^o ^ *
DCA,00005 m$IU I^-DCE^B.Ol mg/L; acetone;
cyclotaanej carbon disulfide; syclopentans, isoKjetaue;
MEK; methylcyelohexane; itiethylcycIopentaneiMIBK,
jieDtane^TCE O.S05 mgft/, toluene, 0.1 rag/U vioyj cflforide,.
Ottemg/tixyleB^USmg/i,) ^ ( '< ^
Volume^groaniwater) 4 gallons )>erimm!te" '
^Volume (feuW watery 1 gallon pef irtiwt* x I ^ '-.
•(. (. " ^ ^ <• \
Yadose Sflit (diesef and gasoline, 1 ing/kg; grease, SO rag/Kg;
0j?,5QwgA«fTPW x < x . t< * - ^ . '
V«It»a»: yOO-cubVjards, C" .^ ~ \'
'I < ' * v ^ *-.
" x\^ «. ° ° x ,r° -
Ground water:4dicbloroniethane»caiNiitetrachfatrebp>
cW«oC«n|; fcenzeite; j,2-di«b1«!0p»i>pe, j*eiio!s, JCB,
totaenejTOyldbioRde) ' < x s<, x
Other contaminants; arsen|c>'cgdininRil eBioinjM%<}yaiBde, ^
fend, Mercury ^ • ' * XN^ ^" ^_xx
Volumei, 50,000 gailons per day - " ^* \
% of total vol atate- 100% ^ -..x^ Nx x-'
Soil (bettzenp, 1 jng^g, ethylbenzene, 50 mg/ftg; toluene',
50 rag/kg; TRPHs; xylene, 50 mg*g) > * ^
Basts; state regulations •, ^^ "•
^ *. '
Soi|x(bydrocarbons, 100 Tg/feg)x
Volume. 70 cubic yards N *
\ ^> j
Soil (bettzete; ethylbenzeke, tolueite; TRPHs, xylene)
Basisi; regslations \
5 0 ^ ^
*v ' " x
STATUS
Full-ccale remediation is planned. Pilot-scale
studies were completed 04/94. Started 04/93.
Full-scale remediation js planned. Pilot-scale . .
studies were completed 01/94, Started 01 193.
Full-scale remediation is planned. Pilot-sea^
studies weie completed 01/94 Started 01/93.
Full-scale remediation is planned. Pilot-scale
studies have been underway s!nee95>/93,'
^ *
t * *
^ > x
S ' ' x
,,-"'*,. "X > o i
Full-scaiereraetiKttioE is pfiinned.Cutienrty being' .
WStallea. \ ~Cl* C "'^ ,<•
°V,*N, > * /-
^ =?*v<^ ^ "<
' ~~ ~ f ' v " ^
Full«sea!e FeHJediadonlras been underway sajce- ,
Ot/ST.^i^-^catestiidtes.haiVfeljeeftceTn^llted^ i
X X "' ' ,?<^^ '
x *> - v- f •"
-<.•'"". v . -l 'x -
Fuli-scate fejoremiediafloi) is not planned,
V*X *\ ^^v^
x % *4 N'
F«H-»>8le>Tenie<(NioB -vs^ coropfeted ftl/89.
Started H/88
s \ ^
^ ^ N ^ c,
Fall-scateremedtatioa is planned. Pifot-seale
studies nave been underway sincef)S®4.
TREATMENT
In situ treatment, bioventing. Aerobic conditions, indigenous
organisms.
In situ treatment, bioventing. Aerobic conditions, indigenous
organisms.
In situ treatment, bioventing Aerobic condiaons, indigenous
organisms.
In situ treatment, ground-water bioremediation. Ex situ
treatment, jjxed film Oxygen source, nntrlent addition
(nitrogen and ortho-phosphate), completely mixed flow.
Aerobic conditions, indigenous organisms. v
> ^
**'•*' v ^ . ^ <. * *
& sitAnd.trealmgnt. AerdMe eondifionsj exogenous an<{ , '
itjoSgeaousorgsiitstts ^ , , > •
* ^ " .v ' s % "•• , ! "- '-.
--.-. xVx '. * r ^-"' v •
BxjslwtreatnpittilaK%^fced,fiO!Jipfetelys«nlxed,'flow. »v ,^
Attobfo«(maitfow v °* V f . ^*> ^
"(^ *• v't*^ <•
\v i ^N N >v ^-o
\ %^ ^"^ * -v1^ v^v^-fr
•-, x x"C"'1"' ' ^ ^ '•j
In sit»treaajient,l)!Qventlng^AerobiC: conditions, indigenous
organisms. -. -> ^ x
^, K N
In rfwtf«imieflt,  ' v \ < ^' ^
Ih situ treatment; biaventing Aerobic conditions^indigenoas
organisBis.. ^ x _^
\
^ \
Indicates a new site.

-------
                       AFrJLJLUA JL1UINS U* mUKUMUlJlAllOJN  (COnt.)
                    CONTACT/
                      PHONE
                     NUMBER
   MEDIA/
CONTAMINANT
b Indicates a new site.
0 Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

-------
      FIELD APPLICATIONS OF BIOKEMEDIATION (cont.)
REG
IX
IX
IX
DC,,.
\
\
SITE/
LOCATION/
LEAD
East Highlands Ranch"
East Highlands, CA
UST lead (state)
Edwards Air Force Base1"
Edwards AFB, CA
Federal facility
Site 16
Site 21
Site43
Former Bulk Terminal ^
Mocfe$.tO»CA
UST lead (state), total health
careageney < „ >
Former Golden I>e v" „,, .
Carson, CA „ N
TSCA lead (state), state water
board— Los Angeles region x
(TSCAIead covers soil ^. »
eontaminatiSrt, state-water
ground-water contamination.)
Ground-water Ufeatraenf *
Soil tteatnwnt
Former Service Station
Los Angeles, CA
UST lead (state).
CONTACT/
PHONE
NUMBER
Lori Patras
(714)505-1800
Contractor/
engineering firm
Marty Faile
(210)536-4331
Federal organization
LOB Patras
(714,) 505-1800 ,
Contractor/-
engineering firm *
Dqtig;C
X2Q>6)7?4-
tSKSuad water (TSUs) I x ^ !
Basts, MCt
Basis- CA Dept «f Toxic Subtanofes Central LOFT field, x
jnanuai^ - v s s
% of total vol. a.t site; 100% , x ,-
Sotl(li^drocarbons, I00m|/kg) ' ' ' l,
Volume (vaitese an.d sanitated soil)^ 3fc cubic yards
yoluttte (ground water), SOOfc^aBoti^ ° ~-'
STATUS
Futl-scale remediation was compleled 06/94.
Started 02/93.
Full-scale remediation is planned. Pilot-scale
studies were completed 04/94. Started 01/93.
Full-scaleiemedtation is planned. Pilot-scale
studies werecompleted 04/94 Started 01/93 ,.
c,
Full-scale remediation is planned Pilot-scale >
studies were comptetedOl/94 StartedO!/93
Fufl-scafe remediation has been underway sstnce' <
ji/93" ^<: ^ , ^
x ^ x , < ,
JFull*scal& jeisedliaSion js planned. JCuuentiy feeins
completed, x *• « f ^ ^ '
Pilot-scale sntdiBs'are being conducted, ' s
fiili-scalereiimBatiouoaslieeneompfeted ,^
Laboratory-scateand pilot-scjile studies have Been
completed °(s •, * "
Full'scaleremedraaon was completes 03/91 ^
Started H/88 Pupt-scafc audies "were completed
12/88 Started 0U88x x " ' x,
TREATMENT
Ex situ land treatment. Aerobic conditions, indigenous
organisms.
In situ treatment, faioventing. Aerobic conditions, indigenous
organisms.
to situ treatment, bioventing. Aerobic conditions, indigenous
organisms <
fn situ treatment biovenhng, Awobio conditions, indtgenoas
organisms * '
Ih si&i treatment, faioventmg, Nutrient additioa (gaseous l
-to situ treaBaenti air Barging, bioyenfing. Mapient addition ^
(artHnoraa^. Aerobic condition^ indigenaus organisrosf ' , x " •
* 0 -i " •> ^^.V °
^ ^ ^ v \ SJ ^ ^ !^ °
Bxstui iauil ss««n»enl pile. 'Aerobic cQwKtas,racligeTio«>
xasahisms ^ ^v'
•j " ^^Nv< <, ^.
' ' VX " !' " , "
Itt situ treatment, ground-wafer bioremediatK>n,sO!P " *
biorenvediatioi) J>n siStt land treatment), closed loop system.
Hydrogen fieKSade, jnttetent addition (nitrogen and others)
Aerobic condittowl indigenous organisms.
>\ x 'x
b Indicates a new site.

-------
                                                                                             V^JL1  MJJL\JKEjL\LJLLiLJJLJr\. A JLV/I1
 REG
    SITE/
LOCATION/
    LEAD
CONTACT/
  PHONE
 NUMBER
     MEDIA/
CONTAMINANT
STATUS
TREATMENT
   IX
        Fort Ord Army Base
        Monterey, CA
        CERCLA enforcement lead
                      John Chesnutt
                      (415)744-2387
                      Federal organization
                 Vadose soil (diesel; gasoline; toluene; xylene)
                 Ground water (1,2-DCE, 6 ppb; TCE, 5 ppb; TPHs,
                 l.OOOppb)
                 Other contaminants: chlorinated dioxins and furans
                 Volume: 4,000 cubic yards
                 % of total vol. at site:  100%
                                     Full-scale remediation is being conducted.
                                     Pilot-scale studies were completed 05/94. Started
                                     01/85.
                            In situ treatment, soil bioremediation (in situ land treatment).
                            Nutrient addition (carbon fertilizers). Aerobic conditions,
                            indigenous organisms.
   IX
        Gila Indian Reservation
        Bapchule, AZ
        CERCLA fund lead
                      Richard Martyn
                      (415)744-2288
                      Federal organization
                 Soil (parathion; toxaphene)
                 Basis: risk-based
                 Volume: 80,000 cubic yards
                 % of total vol. at site: 100%
                                     Full-scale remediation was completed 07/86.
                                     Started 01/84.
                            In situ treatment, soil bioremediation (in situ land treatment).
                            Hydrogen peroxide, nutrient addition (alfalfa, manure).
                            Aerobic and anaerobic conditions, indigenous organisms.
   IX
        Hamburg Ranch
        Merced County, CA
        CERCLA state lead
                      Christine Holm
                      (916)255-3103
                      State organization
                 Vadose and saturated soil (ODD; DDE; chlorfenvinphos;
                 DDT; endosulfan; methidathion; Monitor; Nemacur;
                 parathion-e; parathion-m; toxaphene)
                                     Full-scale remediation is planned. Currently in
                                     predesign. Remediation expected completion
                                     10/96.
                            Bioremediation treatment not yet established.
   IX
        Harmon Field
        Tulare County, CA
        CERCLA state lead
        Above-ground treatment

        Land treatment
                      Mike Pfister
                      (209)297-3934
                      State organization
                 Vadose soil (DDT, 1 mg/kg; toxaphene, 1 mg/kg)
                 Basis: risk-based
                 Volume: 200 cubic yards
                 % of total vol. at site: less than 1%

                 Soil (a-BHC; chlordane, 1 mg/kg; dicofol, 1 mg/kg;
                 4,4'-DDE, 1 mg/kg; 4,4'-ODD, 1 mg/kg; 4,4'-DDT,
                 1 mg/kg; endosulfan IIj 1 mg/kg; endrin, 1 mg/kg; endrin
                 aldehyde, 1 mg/kg; heptachlor, l;heptachlorepoxide,
                 1 mg/kg; methoxychlor, 1 mg/kg; toxaphene, 1 mg/kg)
                 Volume: 65 gallons
                                     Full-scale remediation is planned. Pilot-scale
                                     studies are planned.
                                                                                                            Full-scale bioremediation is not planned.
                                                                                                            Pilot-scale studies were completed 11/90. Started
                                                                                                            05/90.
                            Ex situ treatment, lined cells on aboveground containers,
                            organic material and lime addition. Aerobic conditions,
                            indigenous organisms.
                                                                                                                                           Ex situ land treatment, lime and moisture addition. Aerobic
                                                                                                                                           and anaerobic conditions, exogenous and indigenous
                                                                                                                                           organisms.
 Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

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I
                             FIELD APPLICATIONS  OF BIOREMEDIATION (cont.)
           REG
         SITE/
     LOCATION/
         LEAD
CONTACT/
  PHONE
 NUMBER
     MEDIA/
CONTAMINANT
STATUS
TREATMENT
            IX
                 Hercules Incorporated
                 Hercules, CA
                 CERCLA slate lead
                         Mclidi Sunga
                         (510)540-3825
                         State organization
                Soil (DMT, 5 mg/kg; nitrobenzene, 5 mg/kg; TNT, 30 mg/kg)
                Volume: 3 cubic yards
                                 Full-scale biorcmediation is not planned.
                                 Pilot-scale studies were completed 01/91. Started
                                 01/89.
                         Ex situ land treatment. Aerobic conditions, indigenous
                         organisms.
            IX
Hickam Air Force Base*
HickamAFB.HI
Federal facib'ty
AreaH

ArcaK:
                 Site 2
                                          Patrick Haas
                                          (210)536-4331
                                          Federal organization
               Soil (benzene, 0,05 mg/kg; ctbytbenzcne, 10 mg/kg; toluene,
               7 mg/kg; TRPHs; xyiene)
               Basis: state regulations
                                          Soil (benzene, 0.05 mg/kg; ethylbenzene, 10 mg/kg; toluene,
                                          7 rag/kg; TRPHs; xyiene)
                                          Basis: state regulations

                                          Soil (benzene, Q,05 mg/kg; ethylbenzene, lOmg/Rg, toluene,
                                          7 rag/kg; TRPHs; xyiene)
                                                        ions;              v            '
                                 Full-scale remediation is planned. Pilot-scale
                                 studies were completed 02/94. Started 02/93.
                                                                                                          Full-scale remediation is planned. Pilot-scale
                                                                                                          studies were completed 02/94. Started 02/93.
                                                              Full-scale remediation is planned.l'flot-scaTe"x •
                                                              studies were completed 02/94. Started 02/93.
                         In situ treatment, bioventing. Aerobic conditions, indigenous
                         organisms.
                                                                                                      to situ treatment, biovenfing. Aerobic conditions, indigenous
                                                                                                      organisms.
                                                                                                                                                  In situ treatment, bioventing, Aerobic conditions, Indigenous
                                                                                                                                                  organirms.   <-                 "
            IX
                 J.H. Baxter
                 Weed, CA
                 CERCLA enforcement lead
                 Process 1
                 Process 2
                         Kathy Setian
                         (415)744-2254
                         Federal organization
                Sediments (tetrachlorophenol, 1 mg/kg)
                Soil (PAHs, 0.51 mg/kg; PCP, 17 mg/kg)
                Basis: risk-based
                Other contaminants: arsenic, chromium, copper, zinc
                Volume: 21,875 cubic yards
                % of total vol. at site: 90%

                Ground water (PAHs, 5 u.g/L; dioxins, 0.025 ppt; PCP,
                2.2 ug/L)
                Basis: risk-based
                Other contaminants: arsenic, chromium, copper, zinc
                Volume: 150,000 gallons per day
                % of total vol. at site: 100%
                                 Full-scale remediation is planned. Currently in
                                 predesign. Laboratory-scale and pilot-scale studies
                                 have been completed.
                                                                                                          Full-scale remediation is planned. Currently in
                                                                                                          predesign. Pilot-scale studies have been underway
                                                                                                          since 01/89.
                         Ex situ land treatment. Aerobic conditions, indigenous
                         organisms.
                                                                                                                                 Ex situ treatment, fixed film, completely mixed flow. Aerobic
                                                                                                                                 conditions, indigenous organisms.
            IX
                 JASCO
                 Mountain View, CA
                 CERCLA enforcement lead
                         Rose Marie Caraway
                         (415)744-2356
                         Federal organization
               Soil(1,1-DCA,0.03mg/kg; 1,1,1-TCA, lOOmg/kg;
               1,1-DCE, 1 mg/kg; 1,2-DCE, 1 mg/kg; acetone, 30 mg/kg;
               benzene, 0.3 mg/kg; chloroemane, 4,000 mg/kg; MEK,
               9 mg/kg; diesel, 10K mg/kg; ethylbenzene, 3,000 mg/kg;
               methanol, 200 mg/kg; methylene chloride, 0.2 mg/kg; PCE,
               7 mg/kg; PCP, 200 mg/kg; TCE, 3 mg/kg; toluene,
               1,000 mg/kg; vinyl chloride, 0.02 mg/kg; xylenes,
               2,000 mg/kg)
               Ground water (acetone, 4,000 ppb; 1,1 -DCA, 5 ppb;
               1,1-DCE, 6 ppb; 1,2-DCA, 0.5 ppb; benzene, 1 ppb;
               methylene chloride, 150 ppb; PCE, 5 ppb; PCP, 1 ppb;
               toluene, 1 ppb; TPHs, 3,000 ppb; vinyl chloride, 0.5 ppb)
               Basis: potential migration to ground water
               Volume: 1,100 cubic yards
               % of total vol. at site: 100% of soil
                                 Full-scale remediation is planned. Currently in
                                 design. Laboratory-scale studies were completed
                                 11/91. Started 02/91.
                         Ex situ treatment, ex situ reactor treatment, batch flow.
                         Aerobic and anaerobic conditions.
                                                                                                                                                                                                         I

                                                                                                                                                                                                         i
                                                                                                                                                                                                         3'
          b Indicates a new site.
          c Indicates that the site has been updated.

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                                                                                  UJF
                                                                                                                                                              (cont.j
 REG
  IX
         SITE/
      LOCATION/
         LEAD
        Koppers Company, Inc.
        Oroville, CA
        CERCLA enforcement lead
  CONTACT/
    PHONE
   NUMBER
                           Fred Schauffler
                           (415)744-2359
                           Federal organization
                    MEDIA/
               CONTAMINANT
                   Vadose soil (PAHs, 0.19 mg/kg; dioxins, 0.03 ppt; furans,
                   0.03 ppt; PCP, 17 mg/kg)
                   Other contaminants: arsenic, chromium
                   Volume: 110,000 cubic yards
                   % of total vol. at site: 30%
                STATUS
                                                  Full-scale remediation is planned. Currently in
                                                  predesign. Laboratory-scale studies were
                                                  completed 01/93.
                                                  Pilot-scale studies are planned.
TREATMENT
                                           In situ treatment, soil bioremediation (in situ land treatment).
                                           Nutrient addition. Aerobic conditions, indigenous organisms.
                                                                                                                                                  Oxygen source, nutrient addition. Aerobic conditions,
  IX
Lawrence Livermore
Laboratory—Main Site11
Livermore, CA
CERCLA enforcement lead
Paul Ko
(510)422-1075
Federal organization
Soil (benzene, I mg/kg)
Ground water (gasoline, 1 mg/L)
Basis (soil): potential to leach to ground water
Basis (ground water): MCL
Volume (vadose soil): 400k cubic yards
Volume (ground water): 120k cubic yards
Full-scale remediation is planned. Laboratory-scale
studies are planned. Expected start 10/95, expected
completion 04/96.
Pilot-scale studies are planned. Expected start
06/96, expected completion 12/96.
                                                                                                                                                                                                               a:

                                                                                                                                                                                                               5°
b Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

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                FIELD  APPLICATIONS OF  BIOREMEDIATION (cent.)
 REG
   SITE/
LOCATION/
   LEAD
CONTACT/
  PHONE
 NUMBER
    MEDIA/
CONTAMINANT
                                                                                           STATUS
             TREATMENT
  IX
      Lawrence Livermore
      Laboratory—Site 300b
      Tracy, CA
      CERCLA enforcement lead,
      UST lead (federal) (The
      CERCLA lead deals with
      ground-water and soil
      contamination from previous
      activities; the UST lead covers
      contamination from leaking
      underground storage tanks.)
      Diesel remediation

      TCE remediation
                Mike Brown
                (510)423-7061
                Federal organization
             Sediments
             Soil (diesel, 100 mg/kg)
             Basis: level to prevent leaching to ground water
                           Full-scale remediation is planned. Laboratory-scale
                           studies were completed 12/93.
                           Pilot-scale studies are planned.
Ex situ land treatment. Oxygen source, nutrient addition.
                                         Sediments
                                         Soil (TCE, 1 mg/kg)
                                         Ground water (TCE, 5 mg/L)
                                         Basis (sediments): level to prevent leaching above MCL
                                         Basis (soil): level to prevent leaching above MCL
                                         Basis (ground water): MCL
                                         Other contaminants: TBOS (a silicon-based lubricating oil)
                                                                     Full-scale remediation is planned. Laboratory-scale
                                                                     studies were completed 12/93.
                                                                     Pilot-scale studies are planned.
                                                                                   In situ treatment, ground-water bioremediation, sediment
                                                                                   bioremediation, soil bioremediation (in situ land treatment).
                                                                                   Oxygen source, nutrient addition. Aerobic conditions,
                                                                                   exogenous organisms.
 Indicates a new site.
0 Indicates that the site has been updated.

-------
                                AFFJUIUATIIJINS  U* mUKUMUJJlA'llUIN  (COIH.)
 REG
   SITE/
LOCATION/
   LEAD
CONTACT/
  PHONE
 NUMBER
    MEDIA/
CONTAMINANT
STATUS
TREATMENT
     JSTfifearf
  IX
      Montrose Chemical
      Corporation of California
      Torrance, CA
      CERCLA enforcement lead
                Nancy Woo
                (415)744-2404
                Federal organization
             Vadose soil (DDT)
             Other contaminants: benzene, chlorobenzene, chloroform
                           Full-scale bioremediation is not planned.
                           Laboratory-scale studies were completed 03/94.
                           Started 09/92.
                     Ex situ land treatment. Aerobic conditions, exogenous
                     organisms.
                                                                                                                                                               31
                                                                                                                                                               I
* Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

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                  FIELD  APPLICATIONS  OF BIOREMEDIATION  (cont.)
REG
         SITE/
     LOCATION/
         LEAD
CONTACT/
  PHONE
 NUMBER
    MEDIA/
CONTAMINANT
STATUS
TREATMENT
 IX
      Moore Aviation
      Colusa, CA
      CERCLA stale lead
                         Christine Holm
                         (916) 255-3103
                         State organization
               Vadosc soil (DDE, 1 mg/kg; chlorpyrifos, 2 mg/kg;
               disulfoton, 0.1 mg/kg; 2,4-D, 1 mg/kg; 2,4,5-TCPA,
               0.1 mg/kg; atrazine, 0.03 mg/kg; endosulfan 1, 7.4 mg/kg;
               endosulfan II, 7.4 mg/kg; parathion, 3 mg/kg; propazine,
               0.14 mg/kg)
               Basis: beneficial use water quality criteria
               Other contaminants: bis(2-elhylhexyl)plhalate, phenols
               Volume: 75 cubic yards
                                Full-scale remediation has been underway since
                                09/91. Laboratory-scale studies were completed
                                01/90.
                                Pilot-scale studies have been completed.
                         Ex situ land treatment. Aerobic and anaerobic conditions,
                         indigenous organisms.
 IX
      Naval Air Station— Fallon
      FaUon,NV
      Federal facility
                         Ron Hoeppel
                         (805)982-1655
                         Federal organization
               Vadosesoil
               Saturated soil (benzene; ethylbenzene; toluene, xylene)
               Vadose and saturated soil (JP-5 jet fuel)
               Ground water (JP-5 jet fuel; ethylbenzene;
               1-methylnaphthalene; benzene; n-butylbenzene, naphthalene;
               ;p-xylett& toluene)         '  „
                                Full-scale remediation is planned. Currently in
                                predesign. Laboratory-scale studies have been _
                                underway since 09/93. Expected completion 09/95,
                                Pilot-scale studies have been underway since
                                01/93. Expected completion 06/96
                         to situ treatment, bioshirping. Oxygen source. Aerobic
                         conditions, indigenous organisms.
                                                Olh$r contamkantsr arsenic,
                                                Volume: 20,000-cubic yards
                                               , % of total vol ai Site: t3*
 IX
-Naval Weapons Stafion-^-Seal
Beach     ^    ^   '„
Seal Beadi, CA
JFederariacility (State priority
list Site)     *
                              .Carmen LeBron
                               (805)982-1616
                                                             Full-scale remedia&Mits planned Currently in
                                                             predesigtt Laboratory-scale stntiies have been.
                                                             completed ^ v ,
                                                             Pilot-scale studies have been underway since
                                                             12/92. Expected cornplehort 12/95, _
                                                                       In siftt treatment, gtoHnd-waterbioremedtafion. Nutrient
                                                                       addittoft Aerobic and .anaerobic conditions, indigenouV <
 IX
Navy Exchange Gas Station*
San Ago, CA  s  -  *
Federal facility, V S. Nftyy
                               {$!&) 556^6438
               Basis ^nsfc-based
               Voiume. 2,905 «abw yard?
               % of total VoL° at site 10»
                                                                                             Full-scale reinediatKMiis planned Curteiitlym
                                                                                                        '              '
                                                                       In situ treatment, bioventeig Nuwent addition (nitrogen,-
                                                                       phosphpnis). Aerobic condirjonsfexogenous organisms.1
      sitejs a non-N?l< Federaf
      facility, the US Navy has"
      but must obtatrfapproxal for
      'cleanup activities-through the
      Stste EPA^ which fimctions as
      theiead regulatoty agency.)
      Fire trataingtaeility

      Gas station
      Parfcingtot
                                          -Vatiose soil *   x
                                                             Rill-scate reraedtaaonjis platmed. Currently in
                                                                       Tnsiw tteatment, Sioventtng» Nutrient addiSon (nitrogen, N
                                                                       phospliorus). Aejobic conditions, exogenous organisms".
                                                                       iBi-sita treatment btoventing Nutrient addition {nitrogen,
                                                                       phosphorus), Aerobic conditions, exogenous organisms
Indicates a new site.

-------
REG
  IX
    SITE/
LOCATION/
   LEAD
       Poly-Carb
       Wells, NV
       CERCLA fund lead
CONTACT/
  PHONE
 NUMBER
                    Robert M. Mandel
                    (415)744-2290
                    Federal organization
     MEDIA/
CONTAMINANT
                Soil (cresol, 10 mg/kg; phenols, 20 mg/kg)
                Basis: performance-based
                Volume: 1,500 cubic yards
                % of total vol. at site: 100%
                                                                                                               STATUS
                                 Full-scale remediation was completed 09/88.
                                 Started 06/87. Laboratory-scale studies were
                                 completed 05/87. Started 03/87.
                                                                                                                                                         TREATMENT
Ex situ land treatment. Aerobic conditions, indigenous
organisms.
b Indicates a new site.
c Indicates that the site has .been updated.
Shading indicates a non-CERCLA site.
                                                                                                                                                                                                  i

-------
      FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
IX
- IX
slX ,
IX
IX
OR,
3X>
SITE/
LOCATION/
LEAD
Stadia National Laboratories'1
Livcrmore, CA
San Francisco Bay Region 2,
California Regional "Water
Quality Control Board
Ex situ land treatment
In situ land treatment . .
Seaside High School ^
Seaside, CA
,USTfea.d
Ifratner Jarttfioa, CA
^SteteLeadv / N ^
•f "" ^"<° --"'<* ,
.>>.."- - ." ,
Solvent Service
San Jose, CA
CERCLA state lead
Southern Pacific
Transportation Company
Roseville, CA
CERCLA state lead
StoneCowimer*" "-" N r^', s
Flagstaff, AZ V ' ' '
WTIfad (state) ,'\
Travis Air Force Base11 ^
Trans AFB, CA " <
Federal facility •"",-•
IRPSitet. -~N °
O " "^ N P s V N
SOS Site " -X
CONTACT/
PHONE
NUMBER
John Gould
(505)845-6089
Federal organization
DickEricksson
01.6)322-7046 ",
State organtzaiwn.,
Brace LaBelle " ,\
($^6)324^2958 V
StateotianJzafion „•„
° \ s'
1s 0 * > s
Tony Mancini
(510) 286-0825
State organization
David Wright
(916)332-3910
State organization
Peter Condy^ " x
v(6&2) 085^5524 _-
Technology vendor ,s
SamTafSnder
(210)536-4331^ .
Feciatal organization ,
0 N**^ ^ ^ ^
MEDIA/
CONTAMINANT
Vadose soil/soil fJPH diesel, 50 rog/kg)
Basis: RWQCB directive
Volume: 150 cubic yards
% of total vol. at site: 0.3%
Vadose soil (TPHs, 50 mg/kg) . ." . ;
Basis: RWQCB directive '-••"•
Volume: 3,240 cubic yards
9> of total vol. at site: (>%
Soil (diesel, 500 mg/kg)
Volume: 100 cubic yards N< * ,.
«of»telvoiat*ite:>'10X)% "
Sejf (biphen^l, 1,000 mg«:g; diphenyl ether; J ,000 rog/kgj ,
Basis: risk-based "" , -•*•> '" \ , NN >
"^^/'^-^.^ ^ . /N l ^:\
Ground water (1,2-DCE, 5 u,g/L; cis-l,2-DCE, 6 fig/L;
1,1,1-TCA, 200 tig/L; acetone, 400 ug/L; 1,1-DCE, 1 ug/L;
benzene, 0.7 ug/L; ethylbenzene, 400 ug/L; freon 1 13,
1,200 ug/L; naphthalene, 2,000 ug/L; trans- 1,2-DCE,
10 ug/L)
Basis: not yet established
Soil (hydrocarbons, 5,000 mg/kg)
Volume: 240 tons
VadoseioJI (diesel, 100 rag/kg; fuel oil, JOO fflg/kg)" , ' \ \
Bastss suggested soli cieansp levels ,-^ ' ^ \ * ".
Voiurne: 3^00 cable jards ' ^" ^ ^ x^ -^
Soilc (benzene, } mg*g; ethylbenzene,*50 i"g/kg; Wluene, -
50rog*gtTRPHs;xyfeae,50mg/kg) s\ 1
Basis, state regulations ^ •,,_ •<. ^ <
\ •. •-' - " ^ " " " ' ^ ^ ^ 5 ^ ^ '
Soil (benzene, I nig/kg; ethylbenzene, 50mg/5!g?tolueiK,x
50 mg/kg; TRPHs; xyfene, 50 mg/kg) .
Basis:x$fate regulations -• , , _N \x ••
STATUS
Full-scale remediation has been underway since
04/94. Laboratory-scale studies have been
completed.
Full-scale remediation is planned. Expected start
03/97, expected completion 01/99.
.Laboratory-scale studies have been underway since
03/90.
Pilot-scale studies are planned Expected
completion 01/97.
Full-scale remediation \yas completed 06/88.
Fall«seate remediation has been underway since"
d7/90.jUbcfatory-sc8lfe studies Jwe been \^x '
completed'' \ N \ ^ > '
^)tot^csles^«die?5«re oowpteted 01/90." \. M
Full-scale remediation has been underway since
01/91.
Full-scale remediation was completed 01/91.
Started 11/90.
Fttil-Matewmedraflon hasbe^n attderwaysiacV -
0£>M° ', -> Y' _ x- - ^ ,
FniJ.-scafeTemedfasja'nisplaarBd.^Uot-scale ^ x
swdiesli^yebsen underway since 12/92 i-NS
N ^ N" ^ N \-^ ^^^
Fjill-scaleiemedtarionispknnea.RilQt-scafe s ss
studies have beetfunderway since 1 2&2>y
TREATMENT
Ex situ land treatment. Aerobic conditions, indigenous
organisms.
In situ treatment, soil bioremediation (in situ land treatment).
Nutrient addition (phosphorus, potassium, nitrogen, calcium,
magnesium). Aerobic conditions, indigenous organisms.
Ex situ land treatment Aerobic conditions', indigenous
organisms, > ' .
-Ex sita treatment, pile. Aerobic conditions, indigenous \
organisms, ,'-.*;., \ s * ^ ' '" , • I
- ;' S*"'s < , \ . - ^ 4^ ->'o - ^ '\k s ;
" ' ' ^ ^^^ / ' - " ^ J NN S
VN^S "" s \^ " " '•^ ° \ "\s 5
Ex situ treatment, fixed film, completely mixed flow.
Anaerobic conditions, exogenous organisms.
Ex situ land treatment.
Ex'sfta land teatment'Nuuient additiap {Py»4
-------
                          /vr.r.LJ.^/\rjxF.m>3
 REG
  SITE/
LOCATION/
  LEAD
CONTACT/
 PHONE
 NUMBER
   MEDIA/
CONTAMINANT
STATUS
TREATMENT
                 tation
 Indicates a new site.
c Indicates that the site has been updated.
Shading indicates a non-CERCLA site.

-------
        FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
X


X „


»N
^
SITE/
LOCATION/
LEAD-
Helson Air Force Base1"
BelsonAFB.AK
Federal facility
Site 10
ST-48E2
ST-48E3
Eslmendorf Air Force Base*
ElmendorfAFB.AK
Federal facility v
Pump House HI
•SiteST-61 ^. • -
*• -\ \
3 ^ ^ \ ^ >
StteST-71 " - x
Ya)v<;Pit3-4 v „ °s
\ ^ "*
CONTACT/
PHONE
NUMBER
Lt. Co). Ross Milter
(210)536-433)
Federal organization


Jerry Hansen
(216)5364331,
federal oTganizafiou "
> % *
- X X - .
< X "
x ^ s
MEDIA/
CONTAMINANT
Sot) (benzene; eftylbenzeue: lohiene; TRPHs; xylene)
BUSI'E: state regulations
Soil (benzene; ethylbeiaeoe; toluene; TRPHi; xylene)
Basis: slate regulations
Soil (benzene; ethylberaene; toluene; TgPHs;>ykne)
Basis: state regulations
Soil (berBene^ethylbenzene; toluene; TRPJfs; xylette)
Basis: state rejprfafions
VS>^ N ^ 't'r'\S
Soil (benzene; ethyjbenzene; tolttene; TRPHs; xylene) >
V > ' i. ^ ,
vs ' •» ', ^ ^ '• \ N X' X ., ,
Soil (benzenet.ethylbenzsne; toluene"; TRPHs; xylene)
Basis: state regnlafions " N " * " " * x
v " -., ^ - "* ' I
Soil (benzene; ettiylbenzene; toluene; TRPHs; xyfetw^ *"
Basis: state regulations "';", $
STATUS
Full-scale remediation is planned. Pilot-scale
studies have been underway since 08/92,
Full-scale remediation is being conducted.
Pilot-scale studies have been underway since
09/92.
Full-scale remediation is bei ng conducted.
Pilot-scale studies have been underway since
10/92.
Full-scale remediation is planned Pilot-scale \
studies have been underway since 06/93 , „
F«li-s«ale remediation is planned, Pilot-scale' V
studies have been underway sin.eebf>/93. -s \
< ^ c
Fall-scale remediation is planned. Pilot-scale
studies have been underway since 06/93, Ka s-, * t
^ s ps " "
Fall-scaie remediation is planned Pilot-scale ^ %N~
studi^have been underway since 06/93 , ^ -.
TREATMENT
In situ treatment, biovcnting. Aerobic conditions, indigenous
organisms.
In silu treatment, bioventing. Aerobic conditions, indigenous
organisms.
In siw treatment, bioventing. Aerobic conditions, indigenous
organisms.
IB situ treatment,, bioventing. Aerobic conditions, indigenous
organisms.
In situ treatment, bioventing, Aerobic conditions, indigenous
organisiftSf
s '
fa sitalreatment, bioventing Aerofec CQndittons, indigenous o
organisms, ° ' f \ '-,*•-
1 %N }s ^>s\ss ^
In situ treatrnent, bipventirtg^ Aerobic conditTons, indigenous >
organisms. •> . s , *.
oo
o
 b Indicates a new site.

 c Indicates that the site has been updated.

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                                CONTACT/
                                  PHONE
                                 NUMBER
b Indicates a new site.
c Indicates that the site has been updated-.
Shading indicates a non-CERCLA site.
                                                                                                                                                                                      I

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      FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
X
X
X
X
X
\
X
\
x c
X
if4$
f:-Js-
!-\"V">;
SITE/
LOCATION/
LEAD
Galena Air Force Base1"
Galena AFB, AK
Federal facility
Million Gallon Hill
Power Plant
.•'.;''' . . -
:Hanford,O.S.DOBl>,
Richiand,WA
Federal facility
J H^'Baxter Company
Renton.'WA
State Lead
\
MountatnsHome — Atr Force
Baseb
Mountain Home APBT ID %
federal facility ^ * ^
\ \ v
Texas Tower < ^
ForrGreeiy, AK -
Federal facility •• xN
s
•* V \
tinocal-^eaitie Marketing v v
Terminal ^N s - St
SeattfcWA -s;
'State toxics, PRP lead
-, -s ,,\ N
Utah Power «ud light
Idaho Ms, ID
J?CRA lead (state)
c N ^
Wyckoff Eagle Harbor
Puget Sound, WA
CERCLA enforcement lead
B|H £bfe:Gori^yiitt>. ^C!-x"'-
;Cuml$ Bntjsi Cbtembiag;:^;:-
Ewh»ntnent Canai&V^ ' ", '* :'• '
-•>V , V," ^J,' >-V,r "'.>lt\'*'C :- <
CONTACT/
PHONE
NUMBER
Lt. Col. Ross Miller
(210)536-4331
Federal organization
Daniel B.Anderson
(509)376-9428
Contractor/
^engineering firm
Gail Colbum-
{206)649-7058
State organization
s \
"k
Lt Col Ross Miller %
(210)536-43-31 ^
Federgl organization
\
Paul LSOerty
(907)753-5719
Federal wganizattott
\v x \
\ s
^
^ \^ ^ ^
\ \
Nnamdi Msdakor
<206) 649-7112 • '
Sfate qrgantzatjon

Judith Myers
(208)334^5898
^Stateorganizafon x<
Rene Fuentes
(206)553-1599
Federal organization
"^^S^ft^1
i§^|p^$
f^iS^-'f^5^
MEDIA/
CONTAMINANT
Soil {benzene; cthylbenzene; toluene; TRPHs; xylene)
Basis: state regulations
Soil (benzene; ethylbenzene; toluene; TRPHs; xylene)
Basis: state regulations
Ground water, (carbon tetrachloride, 5 ng/L; nitrate, 45 rng/L)
Volume: 50,000 cubic yards
% of total vol at site, less than JS&
Sedittwnts&Iudge/ground^ water (PAHs; POP; TPHs)
Vadose and saturated soil (PAHs, 1; BAP, 0 13 mg/kg; PCP,
8 3 mg/Kg, TPHs 200^nigflcg)
Basis' risk-based - > o
Volume: 20,000cubic7ards i , ' - "•
Sod (benzene, ethylbenzene; toluene; TRPHs^tene) <.
Basts, state tegalarions ^ - ;
\ \
- " X * ",
• KX \X •«
Vadose Sffll(diese1 lOOmgTkg) " " " _ N^
Ground waterXdtesel, 0.005 mg/L) x v '
Basis (Vadose soi!) State regulation x, ,
Basts (ground water)! MCL for benzene N x^
Votai»4 3,000 cubifi yards j- s " --
% of total vol at site: 100 ^ • " ^ x
o V* ^
N ™
Soil (hydroearbons) xs . ' ,
Volume, 34VOOQ cube yards
% of total *jl.«tsite-4Q% ^ v- , •• *
v \ J N^ — s " ^
^ ^
Vadose soil (fAHsrSO j»g*g)\
Basis>permit standards N • •. \
-^ j ° " ^^XxsS
N v „* ^-x "
Ground water (PAHs, 20 (ig/L; PCP, 6 ng/L)
Basis: water quality criteria
jVaaSse^^CJ^y f -ft3^K::;fi1:> ^S§^ \?-SS
IVotto^^fe^r^^^"^:;^?^^
^bf^ %^^^^vJ^r^^CS*';
STATUS
Full-scale bioremediation is not planned.
Pilot-scale studies have been completed.
Pull-scale bioremediation is not planned.
Pilot-scale studies have been completed.
Full-scale remediation is planned. Currently in
predesign. Laboratory-scale studies were :
completed 01/95. Started 01/92.
Pilot-scale studies have been underway since
10/93. Expected completion 10/96
Full-scale remediaflon is planned. Currently in
design. Remediation expected completion 10/98.
Laboratory-scafesludies werccompfcted 1 1/92,
Starte* ° \ •
^ j ' x ' ^"
\ ' =
"^ V ^
F«ll-scstevi«B>edia8wtl»SSbeenconigIeted, ,
JUfaoratory-scafeaad pitot-scate studies have seen
completed. x s
K X So, » . .
Full-scale bioreinediatioii is not planned • " ' v
Riot-scale studiesls^re completed 07/91:
\ ^ " x'
> V :
Full-scale remediation has been underway since
01/89.
.-F^l-|^e^me$iattipiri§ being cbn(Iacte"d.^l'-4r^''^
'^teratbry-scaiiAafes MvlbeSBiindef^ay-sinte-?
|^^;Bj^ted;fco^^oii^^/--;>r*'---vS-§,v!'i
TREATMENT
In situ treatment, bioventing. Aerobic conditions, indigenous
organisms.
In situ treatment, bioventing. Aerobic conditions, indigenous
organisms.
In situ treatment, ground-water bioremediation. Nutrient
addition (acetate). Anaerobic conditions, indigenous
organisms.
Ex situ land treatment. Aerobic conditions, exogenous and
indigenous organisms.
> /" \ > *
In situ treatment; bioventtng^Aerobic conditions, indigenous
organisms, v v - ^ ' „
In situ treatment, mrspai^rag Oxygen source Aerobic^ '
condiftons, indigenous organisms.. VK ^ ^
* * ^ N ^ ^ ^
* ^ ' ,
^ ^ ^ \
> x • •• ' «•
JEx situ Sand treatment, \
^ *• x 1
^ s -
Tn sito Jwitment, soil bioremediatton (in situ land feitrnent)
Aerobtc''candrtions, exogenous organisms s ^
v ' 1 - , " ° * ^x ^ >"
" < > i
Ex situ treatment, physical separation of wastes and water
with activated carbon addition, completely mixed flow.
Aerobic conditions.
$!$%%;.'<<£!^^
b Indicates a new site.

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                      r J.H/JL/JL/
    REG
    SITE/
LOCATION/
   LEAD
CONTACT/
  PHONE
 NUMBER
    MEDIA/
CONTAMINANT
STATUS
TREATMENT
    "-tntl?
        tricai' Station'-,
                      o     .    ,
            monsttatjoirof Site f,,<'^ -i?c\*
         -Jlemedlattpii.techiiplogy >'-':•- 't
         ^Trenton, Ontamv'Cangcfa*,);^  .
         xEnvifonnienf Citiayfc W*°'' ••'
         '. 'l •  '  " °',^ ;• ^- %fe". ••f/'S'i' \* ' '-
00
OJ
                                  •pedft^i [QC la"tibBal)>i
                                                                                                   
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       FIELD APPLICATIONS OF BIOREMEDIATION (cont.)
REG
Intl
IntI
Intt
(ml
SITE/
LOCATION/
LEAD
Hydro-Quebec/Alma6
Quebec, Canada
Environment Canada
Development and
Demonstration of Site
Remediation Technology
(JDeSRT) Program
Hydro-Quebec/Verdun11
Quebec, Canada
.Environment Canada
Development and
Demonstration of Site
Remediation Technology
(DeSRT) Program _
La Cie Huntsman dir Canada11
Mansonvilte, Quebec, Canada
EnyironmentCanada - ^
"Development and
Demonstration, of Site
Remediation Technology
(DeSRT) Program <•
Met Toronto and Region
Conservation Authority!' ^
Easf-York, Ontario, Canada
Environment "
Canada/Environment Ontario
Development and s \
•Demonstration, of Site < "
Remediation Technology
t (DeSRT) Program ;
CONTACT/
PHONE
NUMBER
Yvan Pouliot
(418)653-4422
Contractor/
engineering firm
Yvaa Pouliot
(418)653-4422
Contractor/
engineering firm
Pierre Beaudry /
(514)292-3333
Industry
Cr GodknowV x
Itamunoala
t9Q5)88J-«f»r
Technology vendor '
-." x>
MEDIA/
CONTAMINANT
Soil (PCP, 5 tag/kg)
Basis: industrial criteria
Other contaminants: inorganic contaminants do not undergo
bioremedialion.
Volume: 500 cubic meters
% of total vol. at site: 10%
Soil (PAHs, 200 mg/kg)
Basis:, industrial criteria
Other contaminants: inorganic contaminants do not undergo
bioremediau'on.
Volume, 500 cubic meters
% of total vol. at site, 1 Q%
Sediments (grease, 1,000 mg/kg; oil, 1,000 rog&g)
Basis: B criteria for soil '
Volume'- 1,350 cubic meters
=% of total vol. autelOOjk v
« ' x \
\ \ \ . \
0^ -,
TREATMENT
Ex situ treatment pile. Aerobic conditions, indigenous
organisms.
Ex situ treatment, pile. Aerobic conditions, indigenous
organisms.
tn situ treatment, in situ slurry reactor. Oxygen source,
nutrient addition {mtrogen, phosphorus, potassium), *
'biosurfactants, completely mixedflow. Aerobic conditions,
indigenous organisms, - ' ' ,
Bx sihi iieamrent, mobile "slurry" reactor wtt> percerit liquid ,
less than or eqtoil (o 30%, batch flow Aerobic conditions,
m&genotis organisms ^ ^
* ^ - -'•-•' "X N * f -
< / " ° " - \ ° -- ~~
\ .. •. ^ ^ •> N ' -
\^ ^ ' ^ .
^ ~> ^
00
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                                                         Ol'

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                                                                             Bioremediation in the Field
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                                           85

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r
                         FIELD APPLICATIONS OF BIOREMEDIATION  (cont.)
REG
Im!
Ittti
Intt
SITE/
LOCATION/
LEAD
Sorel Gas Station11
Sorel, Quebec, Canada
Development and
Demonstration of Site
Remediation Technology
(DeSRT) Program
Vanier Gas Station*
Vanier, Quebec, Canada
Development and
Demonstration of Site
Remediation Technology
(DeSRT) Program
Whitehorse Airport11
Whitehorse, Yukon Territory,
Canada
Environment Canada and
Transport Canada '
CONTACT/
PHONE
NUMBER
Marline Hirou
(514)356-7980
Industry
Martin* Hirou
(514)356-7980
Industry
Frank Kiss
(604)270-5164
Industry
MEDIA/
CONTAMINANT
Soil (benzene, 5 mg/kg; etnylbenzene, 50 rog/kg;
hydrocarbons; toluene, 30 mg/kg; xylene, 50 mg/kg)
Basis: regulatory
Volume: 40 cubic yards
% of total vol. at site: 17%
Soil (benzene, 5 mg/kg; elhylbenzene, 5Q mg/kg;
hydrocarbons; toluene, 30 mg/kg; xylene, SO mg/kg)
.Basis: regulatory
Volume: 2,l50cubic yards
% of total vol. at site; 94%
Soil (BTEX, 50 mg/kg; TEHs, 2,000 mg/kg)
Basis' risk-based
Volume: l,IOOcubicweters
^ •""s
STATUS
Full-scale remediation was completed J 1/93.
full-scale remediation was completed 08/92.
Full-scale remediation was completed 09/94.
Laboratory-scale studies were completed 09/93,
Starred 08/93.
, f. v
TREATMENT
la situ treatment, bioventing. Aerobic conditions, indigenous
organisms. .
In situ treatment, bioventing. Aerobic conditions, indigenous
organisms.
Ex situ land treatment Aerobic conditions, indigenous
organisms.
                                                               GLOSSARY OF BIOREMEDIATION TERMS
              Growth Conditions
              Aerobic-ln the presence of oxygen. ,
              Anaerobic-ln the absence of oxygen.

              Source of Microorganisms
              Indigenous-Occumng naturally at a site.
              Exogenous-Not native to a site.

              Treatment in a Reactor
              Activated Sludge-The biomass is suspended in liquid, captured in a clarifier, and recycled to the
              reactor; the contact time between the waste and the biomass is controlled by wasting excess biomass.
              Extended Aeration-fhe biomass is suspended in liquid, captured in the clarifier, and recycled to the
              reactor; a long contact time is created by enlarging the aeration basin.
              Contact Stabilization-The waste contacts the biomass suspended in liquid in the first aeration tank and
              contaminants are adsorbed to the clarified biomass; then they are digested in the second aeration tank.
              Fixed fi/m-Biomass is retained in the system by using a static support media.
              Fluidized Berf-Bacteria is attached to a support media, which is fluidized in the reactor.
Sequencing Batch Reactor-This self-contained treatment system incorporates equalization, aeration,
and clarification using a draw and fill approach on wastewater sludges.
Slurry Reactor-Contaminants are treated in a soil slurry (a thin mixture of soil and water) with
nutrients and oxygen added as needed; water and soil must be separated after treatment, but clean soil
is left on site.

Treatment Outside of a Reactor
Aerated Lagoon-The biomass is kept suspended in liquid with aeration.
Land Treatment-Waste is applied onto or incorporated into the soil surface in a facility.
Contaminants are treated with microorganisms typically indigenous to the existing soil matrix;
nutrients, moisture, and oxygen can be added to optimize growth conditions. If the waste remains at
the facility after closure, the land treatment facility becomes a disposal facility.
P//e-This method refers to any noncontainerized accumulation of solid, nonflowing waste being
treated or stored.
Bioventing-Mr is injected into contaminated soil at rates low enough to increase soil oxygen
concentrations and stimulate indigenous microbial activity-without releasing volatile emissions.
In Situ TVea/menf-Biodegradable contaminants are treated by microorganisms within the environment
in which they are found. Most commonly, this process utilizes aerobic processes and involves
delivery of oxygen or other electron acceptors and other appropriate amendments.
3"
f

-------