United States Office of Research and Office of Solid Waste and Environmental Protection Development Emergency Response Agency Washington, DC 20460 Washington, DC 20460 EPA/540/R-95/501 September 1995 xvEPA The Bioremediation in the Field Search System Questionnaire A cooperative effort of the U.S. EPA's Office of Research and Development, Office of Solid Waste and Emergency Response, and regional offices, and other federal agencies, state agencies, industry, and universities to expand „„,„,...,-„,.,„,„ the nation's field experience in bioremediation technologies for Superfund BiuRcmcDIAnON , / " and other contaminated sites.- ' Fiald Initiative Recycled/Recyclable Printed on paper that contains at least 20 percent recycled fiber ------- ------- BIOREMEDIATION IN THE FIELD QUESTIONNAIRE The purpose of this questionnaire is to collect information about sites where bioremediation is being considered or implemented, or has been completed. The following questions request information on the current status of your site. This information will be added to a data base of site information being developed by the U.S. Environmental Protection Agency (EPA) as a resource for EPA and other federal agencies, state project managers, consulting engineers, academia, and industry personnel considering the use of bioremediation. The data base currently contains information on 450 bioremediation sites—a number that is expected to increase markedly over the next few years. Information in the data base is accessible through the Bioremediation in the Field Search System (BFSS), a software application available on EPA's Alternative Treatment Technology Clearinghouse (ATTIC) (703-908- 2138), Cleanup Information (CLU-IN) (301-589-8366), and Office of Research and Development (ORD) (513-569-7610) electronic bulletin board systems. It also is available on diskette from EPA by calling 513-569- 7562. BFSS will allow theiiser to search for information on specific types of sites, such as those treating a particular medium or contaminant or those using a particular treatment technology. A summary of information in the data base also appears in EPA's Bioremediation in the Field bulletin, a periodic update on field applications of bioremediation distributed to over 5,000 individuals. The usefulness of both BFSS and the bulletin depends largely on the accuracy and completeness of responses to this questionnaire. Please answer all questions that apply to your site. For questions that do not apply, answer "NA" For information that has not yet been established, please answer "NYE." If you have responded to previous questionnaires, some answers already are completed. Please verify that the answers are correct and current If anything is inaccurate or has changed, please supply the correct information. Refer to the glossary of terms if necessary. Site Report For: Please Return To: BFEQ Support Personnel Eastern Research Group, Lie. (ERG) 110 Hartwell Avenue Lexington, MA 02173 U.S.A. Public reporting burden for this collection of information is estimated to average 5 hours for first time respondents and 0.5 hours for respondents updating previous responses, including the time for reviewing instructions, searching existing data sources, gathering and maintaining fee data needed, and completing and reviewing the collection of information. Send comments regarding these burden estimates or any other aspect of this collection of information, including suggestions for reducing the burden, to Chief, Information Policy Branch, PM-233, U.S. Environmental Protection Agency, 401 M Street, SW., Washington, DC 20460; and to Paperwork Reduction Project (OMB #2040-0048), Office of Information and Regulatory Affairs, Office of Management and Budget, Washington, DC 20503. ------- PART ONE: GENERAL SITE INFORMATION Part one of this questionnaire (questions #1 through #11) requests general information about the site, including the name and location of the site; names, addresses, and phone numbers of site contacts; the regulatory authority or lead under which the site is being remediated; the predominant contaminant at the site; and a brief site description. *** T»T.T? ASTT TWYTir. *** 'PLEASE NOTE If you have filled out a previous questionnaire, please check to make sure that all printed responses are correct, current and complete. If the site has never used or tested bioremediation and is no longer considering bioremediation, you do not need to fill out the rest of this questionnaire. In either case, we need this information to update our site data base and bulletin, so please check the appropriate box below and send the questionnaire back to us by the date indicated on the front page of the questionnaire. Q All printed responses are correct, current, and complete. Q This site has never used or tested bioremediation and is no longer considering bioremediation. If the site has tested bioremediation at laboratory or pilot scale, please complete the questionnaire, even if the tests were unsuccessful and full-scale bioremediation is not being considered. ------- PART ONE: GENERAL SITE INFORMATION Site Identification 1. Site Name: 2. City: State (Province), Zip (Mail Code) 3. Country: 4. EPA Region (if U.S. site): Site Contacts) Information Supplied by: Name: Address: Phone: Fax: E-Mail: Affiliation: Q Federal (or National) Organization Q State (or Provincial) Organization Municipal (or Local) Organization Industry Contractor/Engineering Firm Technology Vendor Other (specify): _ O Q Q (providing the e-mail address will allow us to send you future questionnakes via the Internet If you do not have this capability or prefer not to exercise it, you do not need to fill in this piece of information.) Additional Government Agency Contact: Name: Address: Phone: Fax: E-Mail: Affiliation: Q Federal (or National) Organisation Q State (or Provincial) Organization G Municipal (or Local) Organization Q Other (specify): 7. Additional Private Sector Contact: Name: Address: Phone: Fax: E-Mail: Affiliation: G Industry Q Contractor/Engineering Finn Q Technology Vendor Q Other (specify): ------- PART ONE: GENERAL SITE INFORMATION 8. Site Lead [In most cases, check only one box. If there is more than one lead, please explain under comments below how the authority is divided, e.g., ground-water contamination is under RCRA jurisdiction; soil contamination is under UST jurisdiction.] United States Sites: Ql CERCIA Fund Lead: Covers Federal Lead. Ql CERCLA Enforcement Lead: Covers PRP Lead, Enforcement Lead for RA, Federal Facility Enforcement Lead. Q CERCLA State Lead: Covers State Lead Enforcement, State/Federal Lead. Ql Federal Facility: Covers DIRP, DOE, other federal agency facilities conducting remedial activities. Ql RCRA Lead (Federal): Federal RCRA enforcement Ql RCRA Lead (State): State authority for RCRA enforcement Q USTLead. Ql TSCA Lead (Federal): Depends on whether the state is authorized for enforcement Q TSCA Lead (State): See TSCA Lead (Federal). Q Other (please specify) '. International Sites: Q Government Lead (please specify) Ql Private Sector Lead (please specify). Comments: ------- FART ONE: GENERAL, SITE INFORMATION Site Characterization/Description 9. How would you characterize the contamination being bioremediated or considered for bioremediation at this site? Check all that apply. G WoodPreserving Q Petroleum Q Pesticide/Herbicide Q Solvent Q Other (please specify) (Please categorize the contamination using one of the named categories, whenever, possible, rather than listing an individual contaminants). The purpose of the question is to allow a broad characterization of the site. You will have an opportunity to list all individual contaminants to be treated in a later question.) 10. Please provide a short description of the facility or contaminated site (e.g., inactive wood preserving site, leaking underground storage tank, RCRA land treatment facility to treat oil refinery sludges): (If your site has a number of separate areas being treated or considered for treatment using the same or different bioremediation processes, please list and describe the separate subsites here.) Other Sites 11. If you are aware of other bioremediation sites not already in BFSS or listed in the "Field Applications of Bioremediation" table in the Bioremediation in the Field bulletin, please specify the site(s) below. Site Name: Site Name: City/State: City/State: Contact [ Contact: ' Phone: ^^ Phone: ------- PART TWO: BIOTREATMENT PROCESS INFORMATION Part two of this questionnaire (questions #12 through #31) requests information about a specific biotreatment process being considered or implemented at the site, including the type of technology being used; the laboratory-, pilot-, or full-scale status of the technology; the media and contaminants being treated; the target cleanup levels; and the performance of the technology. If a biotreatment process is being tested at laboratory or pilot scale, your answers to part two of the questionnaire should apply to the current scale of the technology, not to anticipated full-scale activities. Note that some questions are only applicable under certain circumstances. For example, question #31 is appropriate for cost information for full-scale biotreatment processes only. Please read carefully to avoid questions that do not apply to a particular biotreatment process. *** PLEASE NOTE*** If more than one biotreatment process is being considered or implemented at this site, or if the same process is being used to treat separate areas of contamination, please photocopy and complete part two of the questionnaire^^ each process or separate contaminated area. Please enter the number of separate biotreatment processes at your site: ------- VAB.T TWOs BIOTKEATMENT PROCESS _ OF _ Treatment Technology 12. Please identify one biotreatment process being considered or implemented at this site. (In most cases, check only one technology. Checking more than one technology implies that the two or more technologies are being used together in a single process treatment train. If this is the case, please explain in question #19 how these technologies are used in conjunction with one another.) Ex Situ Processes Treatments Involving a Reactor G Activated Sludge Reactor G Extended Aeration Reactor G Contact Stabilization Reactor G Attached Growth G Fixed Film Reactor G Fluidized Bed Reactor G Sequencing Batch Reactor G Slurry Reactor Q Other (please specify) Treatments Not Involving a Reactor G Aerated Lagoon SolidPhase G Prepared Bed (Land Treatment) G Pile Treatment Q Other (please specify) hi Situ Processes G Natural Attenuation (hitrinsic Bioremediation) G Air Sparging (Biosparging) G Bioventing G Confined Treatment Facility for Sediments G In Situ Ground Water Bioremediation G In Situ Sediment Bioremediation G In Situ Soil Bioremediation G Other (please specify) 13. If the technology is in situ, what amendments are used? G Hydrogen Peroxide G Oxygen G Nutrients (please specify) G Other (please specify) ------- PART TWO: BIOTREATMENT PROCESS _ OF _ 14. If the technology involves a reactor, does the reactor treat the material as a solid, liquid, or gas? Q Solid Q Liquid Q Gas 15. If the technology involves a reactor, how would you describe the reactor flow? Q Batch Q Plug Q Completely Mixed 16. What are the growth conditions for the microorganisms? Q Aerobic Q Anaerobic 17. What is the source of the microorganisms? (Check exogenous only if the organisms are actually brought in from another site, cultured in the lab, or engineered. If organisms are merely moved for application from one-part of the site to another or from a contaminated area to a reactor or other treatment unit on site, then the source is still considered indigenous.) Q Indigenous Q Exogenous Specify type(s) of exogenous organism: ------- PART TWO-. BIOTKEATMENT PROCESS _ OF _ 18. Please check any nonbiological technologies that are being tested or implemented at the site in conjunction with this technology. G Chemical Extraction Q Chemical Treatment Q In Situ Soil Flushing G In Situ Vitrification G Soil Washing G Vacuum Extraction G Thermal Desorption G Other (please specify) 19. Please describe the treatment process in more detail. (If the bioremediation process is used in conjunction with a nonbiological technology, or two distinct bioremediation technologies are being used sequentially in the same treatment train, explain how the technologies are being used together in a single process.) 20. Is migration of contaminants an issue with Ihe use of this technology at this site? If so, what containment or confinement technology is being used? ------- PART TWO: BIOTREAlfMENT PROCESS _ OF _ Contaminated Media 21. Please identify the contaminated medium or media being treated or considered for treatment by this biological technology. O Air Q Soil (please indicate zone below) Q Sediments Q VadoseZone Q Sludge Q Saturated Zone Water (please indicate type below) Q Ground Water Q Surface Water 22&. What texture classification system are you using for soiR Q United States Department of Agriculture (USDA) Q ASTM/Unified Soil Classification System (USCS) Q Canada Soil Survey Committee (CSSC) Q International Soil Science Society (ISSS) Q British Standards Institution (BS1) Q Other (please specify) (Please fill in the information for the corresponding texture classification system only.) USDA Texture Class Q Sand Qj Loamy Sand Q Sandy Loam Q Loam Q Silt Q Silt Loam Q Clay Loam Q Silty Clay Loam Q Sandy Clay Q Silty Clay Q Clay Typical Particle Size Distribution % Clay (<0.002 mm) % Silt (0.002 to 0.05 mm) % Sand (0.05 to 2.0 mm) % Coarse Fragments (>2.0 mm) ASTM/USCS Texture Class Q CH Q SP Q Q CL SW Q MH Q GC Q ML Q GM Q SC Q GP Q Q SM GW CSSC Typical Particle Size Distribution % Clay (O.002 mm) % Silt (0.002 to 0.05 mm) ISSS Typical Particle Size Distribution % Clay(<0.002mm) % Silt (0.002 to 0.02 mm) BSI Typical Particle Size Distribution % Clay (O.002 mm) % Silt (0.002 to 0.06 mm) Other Typical Particle Size Distribution % Clay (< mm) % Silt (_ to mm) % Sand (0.05 to 2.0 mm) % Coarse Fragments (>2.0 mm) % Sand (0.02 to 2.0 mm) % Coarse Fragments (>2.0 mm) % Sand (0.06 to 2.0 mm) % Coarse Fragments (>2.0 mm) % Sand ( to mm) % Coarse Fragments (> mm) 10 ------- PART TWO: BIOTREATMENT PROCESS _ OF 22b. What texture classification system are you using for sediments! Q United States Department of Agriculture (USDA) Q ASTM/Unified Soil Classification System (USCS) G Canada Soil Survey Committee (CSSC) Q International Soil Science Society (ISSS) Q British Standards Institution (BSI) Q Other (please specify) _ (Please fill in the information for the corresponding texture classification system only.) USDA Texture Class Q Sand Q Loamy Sand Q Sandy Loam Q Loam Q Silt Q Silt Loam Typical Particle Size Distribution % Clay (O.002 mm) % Silt (0.002 to 0.05 mm) Clay Loam Silty Clay Loam Sandy Clay Q Silty Clay Q Clay % Sand (0.05 to 2.0 mm) % Coarse Fragments (>2.0 mm) ASTM/USCS Texture Class Q CH Q SP Q CL Q SW Q MH Q ML Q SC Q SM Q GC Q GM Q GP Q GW CSSC Typical Particle Size Distribution _ % Clay (O.002 mm) % Silt (0.002 to 0.05 mm) % Sand (0.05 to 2.0 mm) % Coarse Fragments (>2.0 mm) ISSS Typical Particle Size Distribution % Clay (O.002 mm) % Silt (0.002 to 0.02 mm) % Sand (0.02 to 2.0 mm) % Coarse Fragments (>2.0 mm) BSI Typical Particle Size Distribution % Clay (O.002 mm) % Silt (0.002 to 0.06 mm) % Sand (0.06 to 2.0 mm) % Coarse Fragments (>2.0 mm) Other Typical Particle Size Distribution % Clay (< mm) % Silt (_ to _ mm) % Sand ( to mm) % Coarse Fragments (> mm) 11 ------- PART TWO: BIOTREATMENT PROCESS _ OF _ 23. Please indicate the volume of contaminated media being treated or considered/or treatment by this technology, the total volume of these contaminated media at the site, and the percent of the total being treated or considered for treatment with this process. (The first row has been completed as an example.) Medium Example: Soil (vadose) • Volume Under Bioremediation 2,000 cubic yards Total Volume at Site 10,000 cubic yards Percent of Total Volume at Site 20% 12 ------- PART TWO: BIOTREATMENT PROCESS _ OF _ Contaminants and Cleanup Level Data 24. For each medium, please specify the contaminants being treated or considered for treatment by this bwtreatment process and fill in any available information pertaining to original contaminant concentrations, cleanup levels, and technology performance. Do not fill in information on contaminants not undergoing bioremediation (see question #25). If you need more space, please photocopy the table. (One row has been completed as an example.) The data included in this table for rate of reaction, lowest concentration achieved, and time to achieve cleanup level should be based on whatever scale of testing or remediation you are currently undergoing, e.g., if you are doing pilot-scale testing, these data should reflect pilot-scale results.) Medium (Please select from list In question #21) Example: soil (vadose) Contaminant carcinogenic PAHs Maximum Original Concentration 1,500 mg/kg Target or Required Cleanup Level 160 mg/kg Basis for Cleanup Level Risk-based Rate of Reaction 2 mg/kg/day Lowest Concen- tration Achieved 100 mg/kg Time to Achieve Cleanup Level 2 years • 25a. What other contaminants are present that will not undergo bioremediation? b. Does the presence of the other contaminants affect the performance of the biological technology at the site? If so, how? 13 ------- PART TWO: BIOTKEATMENT PROCESS _ OF _ Status 26. Are LABORATORY-SCALE treatabilily or feasibility studies of this biotreatment process being considered, being conducted, or completed on material from the site? Q Yes, being considered. Q Yes, being conducted. Q Yes, completed. Q No. If so, provide the expected or actual dates for start and completion of laboratory-scale activity: Start: Completion: 27. Are PILOT-SCALE treatability orfeasibility studies of this biotreatment process being considered, being conducted, or completed at the site? Q Yes, being considered. Q Yes, being conducted. Q Yes, completed. Q No. If so, provide the expected or actual dates for start and completion of pilot-scale activity: Start: Completion: 28. Is FULL-SCALE remediation using this biotreatment process being considered, being conducted, or completed at the site? Q Yes, being considered. Q Yes, being conducted. G Yes, completed. Q No. (Please elaborate in question #30.) If so, please check the current stage of full-scale remediation and provide the expected or actual date for the start of each stage: Q Predesign Q Design Q Installation Q Operational Q Completed 29. Please identify any problems or obstacles associated with bioremediation or other significant information on the status of bioremediation. (These might include technical, cost-related, or regulatory obstacles.) 30. If full-scale bioremediation has not been considered or is no longer being considered at this site, please explain why. 14 ------- PART TWO: BIOTREATMENT PROCESS _ OF _ Costs 31. Please indicate the capital costs (startup and construction) and operation and maintenance (O&M) costs associated •with the FULL-SCALE application of this technology. If separate figures for capital and O&M costs are not available, enter the total incurred and expected costs. If this biotreatmerit process is currently operating at laboratory or pilot scale, you do not need to fill in this informatioa Costs Capital O&M Total Per Year NA Incurred Total Expected Please indicate the monetary units used: 15 ------- GLOSSARY OF BIGREMEDIATION TERMS Site Lead Agency or program having jurisdiction over site cleanup. Ex Situ Treatment Processes Treatments Involving a Reactor Ex Situ Process Reactor Activated Sludge Reactor Extended Aeration Reactor Contact Stabilization Reactor Fixed Film Reactor Fluidtzed Bed Reactor Sequencing Batch Reactor Slurry Reactor Process that involves the removal of the contaminated medium or media from its original location to another area for treatment. Processes that involve removal of the contaminated material, mixing, and then replacement at the original site are also considered ex situ. A contained vessel in which biological treatment takes place. Technology in which biomass is suspended in liquid, captured in the clarifier, and recycled to the reactor, the contact time between the waste and the biomass is controlled by wasting excess biomass. Technology in which biomass is suspended in liquid, captured in the clarifier, and recycled to the reactor, a long contact time is created by enlarging the aeration basin. Technology in which waste contacts the biomass suspended in liquid in the first aeration tank and contaminants are adsorbed to the clarified biomass; then they are digested in the second aeration tank. Technology in which biomass is retained in the system by using static support media (e.g., a trickling filter). Technology in which bacteria are attached to a support medium, which is fluidized in the reactor. A self-contained treatment system that incorporates equalization, aeration, and clarification using a draw and fill approach on wastewater sludges. Technology in which contaminants are treated in a soil slurry (a thin mixture of soil and water), with nutrients and oxygen added as needed; water and soil must be separated after treatment, but clean soil is left on site. Treatments Not Involving a Reactor Aerated Lagoon Prepared Bed Pile Treatment Treatment in which soil, sludge, or sediment is mixed with water to form a slurry. The slurry is placed in a lagoon and mechanically agitated so that the biomass is kept suspended in liquid with aeration. Nutrient, oxygen, pH, and temperature conditions are controlled. After the process is completed, the slurry is dewatered and the treated material is disposed of. Process in which waste is applied onto or incorporated into the soil surface in a facility or lined treatment bed. Contaminants are treated by microorganisms typically indigenous to the existing soil matrix; nutrients, moisture, and oxygen can be added to optimize growth conditions. If the waste remains at the facility after • closure, the land treatment facility becomes a disposal facility. Process in which a noncontainerized accumulation of solid, nonflowing waste is treated or stored under controlled nutrient, oxygen, pH, and temperature conditions. 16 ------- In Situ Treatment Processes In Situ Process NaturalAttenuadon (Intrinsic Bioremediation) Air Sparging (Biosparging) Bioventing Confined Treatment Facility for Sediments In Situ Ground Water Treatment In Situ Sediment Treatment In Situ Soil Treatment (Land Treatment) Amendment Process that leaves contaminated medium or media in place for treatment Natural attenuation is the biodegradation, dispersion, dilution, sorption, volatilization, and/or chemical and biochemical stabilization of contaminants to effectively reduce contaminant toxicity, mobility, or volume to levels that are protective of human health and the ecosystem. Air is injected below the water table, creating bubbles in contaminated ground water. The air bubbles contact dissolved and adsorbed contaminants in the aquifer, increasing oxygen concentrations and stimulating indigenous microbial activity. Sparging also causes contaminants to volatilize and be transported to the vadose zone, where they can be treated by another technology, such as bioventing or soil vapor extraction. Injection or extraction wells are used to induce a dynamic flow of air through contaminated soil above the water table. Air flow rates are adjusted to increase soil oxygen concentrations and stimulate indigenous microbial activity without releasing volatile emissions. In some bioventing systems, a nutrient solution is injected with the air or percolated through the soil. Caissons are constructed to contain the sediments in the environment in which they are found. Stirring mechanisms then can be used to agitate the contaminated material, and incorporate microorganisms, nutrients, and electron acceptors. Injection wells can be used to circulate nutrients and electron acceptors through contaminated aquifers. In most systems, ground water is pumped through a recovery well down gradient of the contaminated area, treated to some extent, then reinjected up gradient of the contaminated area with additives that enhance biodegradation. The effectiveness of in situ ground water bioremediation depends on maintaining contact between contaminants and injected amendments. Biodegradable contaminants are treated by microorganisms within the environment in which they are found. This process usually is anaerobic and involves the delivery of electron acceptors and other appropriate amendments to the contaminated sediments. Biodegradable contaminants are treated by microorganisms within the environment in which they are found. This process usually is aerobic and involves injection of oxygen, other electron acceptors, and other appropriate amendments to the contaminated soil. Permeable soils with high moisture content are most appropriate for in situ treatment. Chemical additions such as electron acceptors or nutrients to enhance bioprocesses. Media Sediments Soil Sediments refer to aquifer materials including gravelly sand, clayey sand, and sandy clay originating from sedimentary rock. Sediments also are accumulations of such materials as sand and volcanic ash from Ihe atmosphere; of stream gravel, sand, and mud on the lands; and of gravels, sand, clay, and organic remains on the sea floor. Soil is the loose surface material of the earth in which plants grow. 17 ------- Media (continued) VadoseZone Saturated Zone Ground Water The vadose zone is the region extending from tine ground surface of the earth to the upper surface of the principal water-bearing formation. The saturated zone extends from the upper surface of saturation down to underlying impermeable rock. Generally, the water table forms the upper surface of the zone of saturation. This is defined as the surface of atmospheric pressure and appears as the level at which water stands in a well penetrating the aquifer. Groundwater is water occurring in the zone of saturation. Microorganism Growth Conditions Aerobic Anaerobic In the presence of oxygen. Aerobic metabolism involves energy-yielding oxidation reactions in which hydrogen is transferred to oxidized pyridine nucleotides (NAD andNADP). In the absence of oxygen. Anaerobic metabolism involves energy-yielding reactions in which the final electron acceptor is a compound other than molecular oxygen, such as sulfate or nitrate. Microorganism Source Indigenous Exogenous Occurring naturally at a site. Organisms that are moved from one location at the site to another (e.g. to a reactor) to facilitate treatment of the original contaminated medium are still considered indigenous. Not native to a site. These include organisms that are brought in from another site, cultured in a lab, or engineered. Contaminants and Cleanup Level Data Maximum Original Concentration Basis for Cleanup Level Rate of Reaction Lowest Concentration Achieved lime to Achieve Cleanup Level Maximum level of contamination detected prior to start of bioremediation. Regulatory or other standard that establishes cleanup levels. Reduction of contaminant concentration per unit time (mg/L/day). Lowest contaminant concentration at current scale of remediation, or final concentration if full-scale bioremediation has been completed. Time required to achieve cleanup goals for the entire contaminated area being treated or tested. Treatment Status Laboratory Scale Treatability or Feasibility Studies POotScale Full-Scale Remediation Undertaken in the laboratory under controlled conditions. Studies to test the effectiveness of specific techniques for remediating specific media and contaminants. These studies also identify existing site problems and examine potential remedial alternatives, considering technical, regulatory, environmental, public health, and cost issues: Performed on test plots and control plots under field conditions. If a contaminated area is small enough, pilot-scale treatment may be able to achieve cleanup level goals. Remediation employing a specific technology or technologies to clean up contaminants over an entire site or contaminated area. Full-scale remediation often is preceded by treatability or feasibility studies. 18 ------- |