vvEPA
 NATO  CDSM
 OTAN .  CCMS
NATO - CCMS
           United States
           Environmental Protection
           Agency
            "Office of
            Drinking Water
            Washington DC, 2O46O
EPA-570/9-79-020
CCMS-111
Oxidation Techniques
In Drinking Water Treatment

Drinking Water Pilot Project
Report IIA
Advanced Treatment Technology

Karlsruhe, F.R.G.

         CI2  CI02
     03  KMnO*
   UV-

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           Department of Water Chemistry
                        and
             DVGW Research Department

              Engler-Bunte-Institute
              University of Karlsruhe
OXIDATION TECHNIQUES IN DRINKING WATER TREATMENT
Papers presented at the Conference held in .Karlsruhe,
Federal Republic of Germany, September 11 - 13, 1978
Compiled and edited by
         Dr. -Ing. • W. Ktihn and
         Prof. Dr. H. Sontheimer
Karlsruhe, 1979

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-------
PREFACE
This volume contains the complete manuscripts of all
lectures given by numerous leading scientists and engineers
from many countries at the Conference on

     "OXIDATION TECHNIQUES IN DRINKING WATER TREATMENT"

held in Karlsruhe, Federal Republic of Germany, in September
1978.

This conference was the tenth event in the series of lectures
on special problems of water technology, held annually in
turn at Berlin, Munich and Karlsruhe. It was also a meeting
of the NATO^CCMS Study (CCMS: Committee on the Challenges of
Modern Society) on Modern Problems of Drinking Water Supply.

Due to the participation of so many experts from different
countries, these reports present a complete survey of all
modern problems in the application of oxidation techniques
in drinking water treatment. They also contain the latest
results of practical and basic research as well as an out-
look on future developments.

The editors wish to thank

   the German Federal Ministry of the Interior for the
   financial support

-  the lecturers, discussion-*-leaders and -participants for
   the accurate preparation of their papers and for their
   valuable contribution to the conference

•"  the members of our Institute and of the DVGW Research
   Department who helped in organizing the conference, and

-  Mrs. Use flein and Mr. Bernd Frick for their help in
   compiling these reports for publication.
Karlsruhe, 1979


W. Kuhn    H. Sontheimer

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INTRODUCTION
Many of the industrialized Nations today face problems such
as population,  energy, and protection of the environment.
In order to optimise use of the scientific and technical
expertise from different countries, the Committee on the
Challenges of Modern Society (CCMS) was created between the
Allied nations of the North Atlantic Treaty Organization.
This international society of scientists strengthens ties
among members of the North Atlantic Alliance and permits
NATO to fill a broader social role with non-member
countries.  CCMS has been responding to the increasingly
complex, technological problems facing modern society.

A' particular problem concerning many Nations is organic
chemical contamination in finished drinking water supplies.
This contamination results from industrial and municipal
discharges, urban and agricultural run-off, degradation of
naturally occurring organic material, and probably primarily
in many cases from the reaction of disinfectants such as
chlorine ozone or chlorine dioxide with these substances
during the treatment process.  Some of these chemicals, evven
in the low concentrations found in drinking water,-may be a
human health risk.  In order to address this problem and
benefit from,international collaboration, the United States
Environmental Protection Agency initiated the Drinking Water
Pilot Project.   The pilot study is evaluating the problems
and their solutions in the following six areas of drinking
water supply science and technology:

     I.  Analytical Chemistry
    II.  Advanced Treatment Technology
   III.  Microbiology
    IV.  Toxicology
     V.  Reuse of Water Resources
    VI.  Ground Water Protection

Two major components of the NATO-CCMS Drinking Water Pilot
Project were two international conferences entitled Oxida-
tion Techniques in Drinking Water Treatment and Practical
Application of Adsorption Techniques in Drinking Water.
These Conferences were held in Karlsruhe, Federal Republic
of Germany, between September 9-15, 1978, and in Reston,
Virginia, on April 30, May 1  and 2, 1979, respectively.  The
Reston conference, sponsored by EPA, included presentations
on the toxicology of organic chemicals, the analytical
chemistry of monitoring for organics, and advanced adsorp-
tion treatment technology.  The Karlsruhe conference was the
tenth of a series on the special problems of water technolo-
gy held annually in turn at Berlin, Munich, and Karlsruhe.
This conference was supported by the Federal Republic of

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Germany and was arranged by the DVGW Research Department at
the Engler-Bunte Institute of the University of Karlsruhe,
FRG.  Experts from more than ten countries presented papers
giving a survey of oxidation processes, -reports on recent
results of research, and operational experience gained at
water treatment plants.
                                                          i
Treatment of surface waters for use as drinking water should
include floeculav,ion, sedimentation, filtration, and disin-
fection in order to ensure microbiological quality and low
turbidity.  These two conferences, however, were concerned
primarily with treatment steps that could remove organic
chemicals.  Speakers at the Karlsruhe conference discussed
five different methods of organics removal by the use of
oxidation techniques.  First, tests with different waters
have shown that preozonation can improve flocculation
efficiency.  Secondly, as a result of biological oxidation,
pretreatment of water by the use of storage basins can
increase the removal efficiency of suspended solids.  Third,
the formation of halogenated organic compounds can be
avoided if chlorine dosages are controlled so that there is
no free chlorine residual.  Next, riverbank filtration can
result in a 50-75 percent reduction of dissolved organic
carbon (DOC).  Finally, the advantages of increased
biological oxidation in sand or carbon filters by treating
water before filtration with ozone was also discussed.

This first of two reports from the Drinking Water Pilot
Project, Group II, Advanced Treatment Technology, is a
tribute to the efforts of Professor Dr. Heinrich Sontheimer
and Dr. Wolfgang Kfihn, who conceived and produced it, the
FRG that sponsored it, and to the spirit of international
cooperation for mutual benefit that has been a keynote of
the entire Pilot Project.  We look forward to continuing the
ties that have developed as a result, and to the remaining
reports of this series developed by the Drinking Water Pilot
Project participants.
                        Joseph A. Cotruvo
                        U.S. Environmental Protection Agency
                        Project Chairman
                        Washington, D.C., USA
                        1979

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CONTENTS

                                                      Page



List of Authors                                         7
Sontheimer, H.
     Development, problems, aims, and significance     13
     of the oxidation process in the treatment of
     drinking water
Practical Use of Drinking Water Chlorination
Miiller, G.
     Hygienic significance of chlorination and         68
     the requirements therefrom

Quentin, K.~E.j Weil, D.
     Reaction of chlorine with inorganic               79
     constituents of water

Richard, Y.
     Chlorination - Practical requirements for         89
     its application

Bernhardt, H.; Hoyer, O.
     Characterization of organic water constituents   11O
     by the kinetics of chlorine consumption
     Discussion Papers:

     Lamblin, H.
          The practice of chlorination of             138
          drinking water

     Meijers, A.P.
          Use of chlorine by the Netherlands          141
          waterworks

     Uhlig, G.
          The practice of chlorination of             143
          drinking water

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                             _  O  —.



                                                      Page


Unwanted By-Products of Chlorination
Stevens, A.A.
     Formation of non-polar organo-chloro              145
     compounds as byproducts of chlorination

Kiihn, W.; Sander, R.
     Formation and behaviour of polar organic          161
     chlorine compounds                 '

Chedal, J.; Schulhof, P.
     Reduction of the content of chlorine compounds    176
     by a treatment combining physico-chemical and
     biological processes

Symons, J.M,; Stevens, A.A.
     Physical-chemical pretreatment for the            ,195
     removal of precursors


     Discussion Papers:

     Josefsson, B.
          Unwanted by-products of chlorination         214

     Normann, S.
          Occurrence of volatile organohalogen         217
          compounds in the operation of water-
          works with various types of water and
          amounts of chlorine

     Rook, J.J.
          THM formation in two different water         226
          treatment systems at Rotterdam
Ozone Treatment and Reactions
Gilbert, E.
     Chemical changes and reaction products in the     232
     ozonization of organic water constituents

Hoigne, J.; Bader, H.
     Ozone requirement and oxidation competition       271
     values of various types of water for the
     oxidation of trace impurities

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                                                      Page
Mallevialle, J.
     Transformation of humic acids by ozone            291

de Greef, E.; Hrubec, J..; Morris, J.C.
     Ozone and halogenated organic compounds           309
     Discussion Papers:


     Hoigne, J.
          Note on the haloform formation potential     327
          of pre-ozonized water

     Legeron, J.P.
          The conditions of ozoriization                ,331

     Lienhard, H.; Sontheimer, H.
          Description of reactions by group            334
          parameters
Ozone;  Production, Contactors, Demand
Uhlig, G.
     Production of ozone from oxygen                   339

Gomella, C.
     Measurement of the ozone-demand                   354
     Discussion Papers:


     Masschelein, W.J.                                 _....
          Ozone input
The Use of Ozone in Drinking-Water Treatment
Kruithof, J.C.
     Ozonization by-products and their removal         367
     by coagulation

Maier, D.
     Microflocculation by ozone                        394

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                             -  4  -
                                                      Page
Rice, E.G.; Robson, C.M.; Miller, G.W.; Hill, A;,G.      418
     Practical uses of ozone in drinking water
     treatment
     Discussion Papers:        .•    .


     Chedal, J.
          The use of ozone in the treatment of          451
          drinking water, -.   .    •;

     Kott, Y.
          Synergistic effect of ozone and chlorine      454
          on bacteria and viruses in secondary
          wastewater effluents

     Valenta, J.
          Example of UV ozone cpntrol ,- Elimination     456
          of residual ozone
Application of Other Oxidation Processes
Masschelein, W.J.
     Use of chlorine dioxide for the treatment         459
     of drinking water

Hedberg, T.; Josefsson, B.;. Roos, C-; Lindgren, B.;    481
Nemeth, T.
     Practical experience with chlorine dioxide and
     formation of by-products,

Berglind, L.; Gjessing, E.; Skipperud Johansen, E.     51O
     Removal of organic matter from water by UV
     and hydrogen peroxide
     Discussion Papers:


     Kirmaier, N.
          Anodic oxidation as a process step in the    524
          treatment of bacterially contaminated water

     Kotter, K.
          Experience with potassium permanganate       528

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                            - 5 -
                                                      Page
     Overath, H.         .
          The use of hydrogen peroxide in water        544
          treatment
     \

     Valenta, J.
          Some aspects of the use of chlorine or       556
          chlorine dioxide in water treatment
Biological Processes in Drinking-water Treatment
Richard, Y.
     Biological methods for the treatment of           56Q
     ground water

Goodall, J.B.
     Biological removal of 'ammonia                     586

KuSmaul, H.
     Purifying action of the ground in the             597
     treatment of drinking water

Piet, G.J.; Morra, C.F.
     Behaviour of micropollutants in river water       6O8
     during bank filtration

Schmidt, K.
     Experience with the removal of micro-^             62O
     impurities in slow sand filters

Roberts, P.V.                 '
     Removal of trace contaminants from reclaimed      647
     water during aquifer passage
     Discussion Papers:

     Chedal, J.
          Biological processes for the treatment       673
          of drinking water

     Halm0, G. ; Eimhjellen, K.; Thorsen, T.
          Nitrogen removal in biological reactors      675
          at low temperatures

     Werner, P.; Klotz, M.; Schweisfurth, R.
          Microbiological studies on activated         678
          carbon filtration

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                             - 6 -
                                                      Page
Combination of Chemical and Biological Oxidation
Processes
van der Kooij, D.
     Processes during biological oxidation             689
     in filters

Sontheimer, H.
     Process engineering aspects in the combination    702
     of chemical and biological oxidation

Jekel, M.
     Experience with biological activated carbon       715
     filters
     Discussion Papers:


     Schulhof, P.
          The use of combined chemical and             727
          biological oxidation processes
Statements


Bousoulengas, A.    (Greece)                            731

Coin, L.            (France)                            733

Dick, T.A.          (United Kingdom)                    735

Heinonen, E.        (Finland)                           740

Kimm, V.J.          (U.S.A.)                            744

Kott, Y.            (Israel)                            751
MUller, G.          (F.R.G.)                            753

Myhrstad, J.A.      (Norway)                            755

Piet, G.J.          (The Netherlands)                   758

StenstrSm, T.       (Sweden)                            76O

Toft, P.            (Canada)                            763

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AUTHORS
                            - ..7 -
H. Bader
Dr. L. Berglind
Prof. Dr. H. Bernhardt
Dr. A. Bousoulengas
J. Chedal
Dr. L. Coin
Dr. J.A. Cotruvo
Dr. T.A. Dick
K. Eimhjellen
Dr. E. Gilbert
Dr. E. Gjessing
Eidg. Anstalt fur Wasserversorgung,
Abwasserreinigung und Gewasser-
schutz (EAWAG), CH-86OO Dubendorf,
Switzerland

Norwegian Institute for Water
Research, Postbox 333, Blindern,
Oslo 3, Norway

Wahnbaehtalsperrenverband,
Postfach 27, D-520O Siegburg 1,
F.R.G.

Scientific Research and Technology
Agency, Vassileos Constantinou 48,
Athens 5O1, Greece

Compagnie Generale des Eaux,
Sie"ge Social, 52 rue d'Anjou,
F-75384 Paris Cedex 08, France

Ministere de la Sante et de la
Direction Generale de la Sante,
20 rue d'Estrees, F-750O7 Paris,
France       ~

Office of Drinking Water, United
States Environmental Protection
Agency, 4O1 M. Street, S.W.,
Waterside Mall, WH 550, Washington,
D.C. 2O46O, U.S.A.

Department of the Environment,
2 Marsham Street, London, S.W.1,
England        .

SINTEF-Dept. of Chemical Technology,
University of Trondheim-NTH,
N-7O34 Trondheim, Norway

Institut ftir Radiochemie,
Kernforschungszentrum Karlsruhe,
Postfach 364O, D-75OO Karlsruhe 1,
F.R.G.

Norwegian Institute for Water
Research, Postbox 333, Blindern,
Oslo 3, Norway

-------
Dr. C. Gomella


Dr. J.B. Goodall



Dr. E. de Greef




G. HalmjzS



Dr. T. Hedberg




t>r. 1. Heinonen



A.G. Hill




Dr» J« Hoigne




Dr. 0. Hoyer


Dr. J. Hrubec




Dr. M, Jekel




Dr. B. Josefsson
S.E.T.U.D.E., 27 Boulevard des
Italians, F-75OO2 Paris, France

Medmenham Laboratory, Water Research
Centre, P.O.Box 16, Medmenham,
Marlow, Bucks. SL7 2HD, England

National,Institute for Water Supply,
Chemical Biological Division,
P.O. Box 15O, AD 226O Leidschendam,
The•Nether1ands

SINTEF-Dept. of Chemical Technology,
University of Trondheim-NTH,
N-7034 Trondheim, Norway

Department of Water Supply and
Sewerage, Chalmers Tekniska
HSgskola; Sven Hultins Gata 8,
Goteborg, Sweden

Helsinki City Waterworks,
Pasilankatu .41, SF-OO24O Helsinki,
Finland

Department of Chemical Engineering,
College of Engineering, Louisiana
University, Ruston, Louis. 7127O,
U.S.A.

Eidg. Anstalt fttr Wasserversorgung,
Abwasserreinigung und Gewasserschutz
(EAWAG) , CH-86OO Dfibendorf,
Switzerland

Wahnbachtalsperrenverband, Post-
fach 27, D-520O Siegburg'1, F.R.G.

National Institute for Water Supply,
Chemical Biological Division,
P.O.Box 15O, AD 226O Leidschendam,
The Netherlands

Engler-Bunte-Institut, Bereich
Wasserchemie, Universitat Karlsruhe,
Postfach 638O, D-75OO Karlsruhe 1,
F.R.G.

Department of Analytical Chemistry,
University of Gothenburg,
S-4O22O Gothenburg, Sweden

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Dr. V.J. Kiram
Dr. N. Kirntaier



M. Klotz



Dr. K. KStter


Dr. D. van der Kooij



Prof. Y. Kott




Dr. J.C. Kruithof


Dr. W. Kiihn




Dr. H. KuBmaul
H. Lamblin
Dr. J.P. Legeron
H. Lienhard
 Office of Drinking Water,  Uniteu ,    *
 States -Environmental Protection
 Agency,  4O1  M.  Street,  S.W.,
 Waterside Mall, WH 55O,        '     :
 Washington,  D.C. 2O46O, U.S.A.

 Institut fur Biomedizinische  Technik,
•Museumstr.  1, D-8OOO Mtinchen  22,
 P.E.G. '.

 Institut fur Hygiene und Mikro-
 biologie, Universitat des Saarlandes,
 B:au- 43,' D-6650 Homburg/Saar,  P.R.G.  • •

 Gelsenwasser AG, Postfach 769,
 D-465O Gelsenkirchen, P.R.G.

.The-Netherlands Waterworks,
 Testing and Research Institute  -
 KIWA Ltd.,  Rijswijk, The Netherlands

 Environmental and Water Resources/   '•'
 Engineering, Technion-Israel
 Institute of Technology, Technion
 City, Haifa 32OOO, Israel

 KIWA N.V.,  Wiers 17, Nieuwegein,
 The,Netherlands

 Engler-Bunte-Institut,  Bereich
 Wasserchemie, Universitat Karlsruhe ,•>'"
 Postfach 638O,  D-75OO Karlsruhe 1,
 P.E.G.

 Institut fiir Wasser-, Boden-  und
 Lufthygiene des Bundesgesundheitsr
 amtes, Aussenstelle Frankfurt/Main,
 Kennedyallee 97, D-6OOO Frankfurt  7O,
 F.R.G. ,                   • •      •  • •".

 Cbmpagnie Generale des Eaux,
 Siege Social, 52 rue d'Anjou,
 F-75384 P'aris Cedex O8, France

 Trailigaz,  29-31 Bd de la Muette,
 F-9514*0 Garges les Gonesse, France

 Engler-Bunte-Institut,  Bereich
 Wassetchemie, Universitat Karlsruhe>
 Postfach 638O,  D-750O Karlsruhe 1»
 F.R.G.

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                           -  10 -
Dr. B. Lindgren



Dr. D. Maier



J. Mallevialle



Dr. W.J. Masschelein



Dr. A.P. Meijers


G.W. Miller



C.F. Morra




Prof. J.C. Morris



Prof. Dr. Gertrud Muller




Dr. J.A. Myhrstad


T. Nemeth


Sabine Normann


Dr. H. Overath


Dr. G.J. Piet
Swedish Forest Products Research
Laboratory, Box 56O4,
S-11486 Stockholm, Sweden

Zweckverband Bodenseewasser-
versorgung, D-777O Wberlingen-
SuQennuihle, F.R.G. .

Societ§ Lyonnaise des Eaux et de
1'Eclairage, 1O rue de la Liberte,
F-7823O Le Pecq, France

Laboratoires C.I.B.E., 764 Chauss§e
de Waterloo, B-118O Bruxelles,
Belgium

KIWA N.V., Wiers 17, Nieuwegein,
The Netherlands  ,

Public Technology, Inc.,
114O Connecticut Avenue, NW,
Washington, D.C. 2OO36, U.S.A.

Chemical Biological Division,
National Institute for Water Supply,
P.O.Box 150, AD 2260 Leidschendam,
The Netherlands

Division of Applied Sciences,
Harvard University> 127 Pierce Hall,
Cambridge, Mass. O2138, U.S.A.

Institut fiir Wasser-, Boden- und
Lufthygiene des Bundesgesundheits-
amtes, Corrensplatz 1,
D-1OOO Berlin 33, F.R.G.

National Institute of Public Health,
Geitmyrsveien 75, Oslow 1, Norway

Gothenburg Water- and Sewage-Works,
Fack, S-4O11O Gothenburg, Sweden

Stadtwerke Wiesbaden AG, Kirchgasse
2, D-62OO Wiesbaden, F.R.G.

Stadtwerke Wiesbaden AG, Kirchgasse
2, D-62OO Wiesbaden, F.R.G.

Chemical Biological Division,
National Institute for Water Supply,
P.O.Box 15O, AD 226O Leidschendam,
The Netherlands

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Prof. Dr. K.-E. Quentin
Dr. R.G. Rice
Dr. Y. Richard
Prof. P.V. Roberts
Dr. C.M. Robson
Dr. J.J.  Rook
C, Roos
R. Sander
Dr. K. Schmidt
P. Schulhof
Prof. Dr. R. Schweisfurth
Dr. Ei Skipperud Johansen
Prof. Dr. H. Sontheimer
Institut fiir Wasserchemie und
Chemische Balneologie,.
Technische Universitat Miinchen,
Marchionistr. 17, D-8000 Mxinchen 7O,
F.R.G.

Jacobs Engineering Group,
1511 K Street, N.W., Suite 835,
Washington, D.C. 2OOO5, U.S.A.

Degremont Traitement des Eaux,
P.B. 46, F-92151 Suresnes, France

Environmental Engineering,
Department of Civil Engineering,
Stanford University, Stanford,
California 943O5, U.S.A.

Department,of Public Works,
2460 City-County Building,
Indianapolis, Indiana 462O4, U.S.A.

Drinkwaterleiding Rotterdam,
Postbus 1166, Rotterdam,
The Netherlands

Department of Analytical Chemistry,
University of Gothenburg,
S-4O22O Gothenburg, Sweden

Engler-Bunte-Institut, Bereich
Wasserchemie, Universitat Karlsruhe,
Postfach 6380, D-75OO Karlsruhe 1,
F.R.G.

Institut ftir Wasserforschung GmbH
Dortmund, Deggingstr. 4O,
D-46OO Dortmund 1, F.R.G.

Compagnie Generale des Eaux,
Siege Social, 52 rue d'Anjou,
F-75384 Paris Cedex O8, France

Institut fiir Hygiene und Mikro-
biologie, Universitctt des Saarlandes,
Bau 43, D-665O Homburg/Saar, F.R.G.

National Institute for Public Health,
Geitmyrsveien 75, Oslo 1, Norway

Engler-Bunte-Institut, Lehrstuhl
und Bereich fiir Wasserchemie,
Universitat Karlsruhe, Postf. 638O,
D-75OO Karlsruhe 1, F.R.G.

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                            - 1 2 ,-'
Dr. T. Stenstr6m
Dr. A. A. Stevens
Dr. J.M. Symons




Dr. T. Thorsen



Dr. P. Toft



Dr. G. Uhlig


J. Valenta


Dr. D. Weil




3?. Werner
Department of Environmental Hygiene,
The National Swedish Environment -
Protection Board, Pack,
S-1O4O1 Stockholm, Sweden

Drinking Water Research Division,
U.S. Environmental Protection
Agency, 26 West St. Clair Street,
Cincinnati, Ohio 45268, U.S.A.

Water Supply Research Division,
U.S. Environmental Protection
Agency, 26 West St."Glair Street,
Cincinnati, Ohio 45268, U.S.A.

SINTEF-Dept. of Chemical Technology,
University of Trondheim-NTH,
N-7O34 Trondheim, Norway

Environmental Health Centre,
Tunney's Pasture, Ottawa,
Ontario K1A OL2, Canada

Stadtwerke Duisburg AG, Postf. 1OO246,
D-41OO Duisburg 1, P.E.G.

Wasserversorgung Zurich, Hardhof 9,
CH-8023 Zurich, Switzerland

Institut fur Wasserchemie und
Chemische Balneologie, Technische
Universitat Miinchen, Marchionistr. 17,
D-800O Miinchen 7O, F.R.G.

Institut fur Hygiene und Mikro~
biologie, UniversitSt des Saarlandes,
Bau 43, D-665O Homburg/Saar, F.R.G.

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                            - 13 -


DEVELOPMENT, PROBLEMS, ATMS, AND SIGNIFICANCE OP THE OXIDATION
PROCESS IN THE TREATMENT OF DRINKING WATER

H. Sontheimer
Introduction
Ever since it was found, first in the Netherlands and some-
what later in the USA (1,2), that in the chlorination of
water - a disinfection and oxidation process used both then
and now in nearly all" waterworks - chloroform and other halo-
form compounds can be formed in large quantities, increasing
attention has been paid both in practice and research to the
oxidation processes in the treatment of drinking water.  This
trend became even more marked after studies on the total
amount of organically bound chlorine, particularly those in
Karlsruhe, had shown that as a rule the amount of this is
greater by a factor of 5 - 10 than the amount of haloforms
or chloroform (3).  A typical result of such work is illus-
trated in Fig. 1.

The figure shows the results from the chlorination of pure
humic acid solutions of various concentrations with the same
amount of chlorine  (20 mg/1) after a reaction time of almost
24 h.

It can be seen that only about one-sixtieth, of the chlorine
added has been converted into chloroform, while the greater-
part of it is bound in polar compounds which can be gas-
chromatographed only with difficulty.  Some of the latter
compounds were only formed by the likewise very considerable
purely oxidative action of chlorine.

From these and many other results, more detailed reports on
which are given in other lectures, it has become evident
that even a process like chlorination, which has important

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                             - 14 -
   2500
  2000
   1SOO
 O>

 M
 o
   1000
   5OO
                                  X*

      TOCl
 	  Chloroform
 chlorine addition: 20 mg/1
                  reaction time:
                    23 h
           1O
20     30     40
HS  Na in mg/1
 Fig.  1   Dependence of chloroform  and  TOC1 on the
         concentration of humic acid
advantages for water treatment, can have  clear  limitations
as regards its applicability, and  that  it is  impossible to
solve all problems of water quality just  by adding  more
chlorine.

As with chlorination and its beneficial and adverse effects,
we are concerned today, induced by the  developments just
discussed, with other similar treatment processes and with
the aims of drinking-water treatment  in general.
An attempt to summarize briefly  the  experience,and  conse-
quences that have emerged  in  the last  few years  can in my
view be reduced to the following points:

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 TABLE  1   BASIC PRINCIPLES  ON DRINKING-WATER TREATMENT
   The  aim of all  measures  in drinking-water treatment
   is to  obtain  a
        "naturally pure drinking water"

   An optimum drinking-water treatment requires the
   use  of suitable processes in a sensible combination,
   with observation of  the  following requirements:

         Reliable  and extensive removal
         of all  harmful substances

   -      Avoidance of new formation or
         enrichment of  harmful substances

         Adaptability to changed properties
         of raw  water
   Processes  for  drinking-water  treatment  may  also  have
   undesired  effects, on the  quality  of  drinking  water
1)     The aim of all measures in the treatment of drinking
      water is to obtain a "naturally pure drinking water".
                                            tr

2)     An optimal treatment of drinking water requires the
      use of suitable processes in a sensible combination,
      with observation of the following demands:

      a)  reliable and far-reaching removal of all.anthro-
          pog.enic and naturally formed perturbing materials,

      b)  avoidance of new formation or enrichment of
          perturbing materials in the course of the treat-
          ment itself,

-------
                              16 -
      c)  reliable' -effectiveness- -even*'when' the properties
          of the raw water are changed,

      d)  simple and effective control of the operation.

3)    The processes used for treating drinking water can also
      have undesirable effects on the water quality if they
      are mis-applied.
In this admittedly  incomplete  list the  last point has gained
particular significance  in  recent years on the basis of the
experience with chlorination,  and has certainly contributed
to the fact that the aims of the treatment of drinking water,
i.e. the quality requirements  for a given drinking water, are
an object of increasing  consideration.  Although we cannot go
into closer details of this problem within the framework of
this lecture, it seems necessary to make some further state-
ments on this question and on  the first point in the above
list.

"Naturally pure" or only "safe" drinking water
In all countries there are laws and decrees ensuring the
supply of "safe" drinking water, i.e. ensuring that the use
and consumption of drinking water has no undesirable effects
on health. To illustrate this point, we may quote the Safe
Drinking Water Act in the United States (4), or the Drinking
Water Act of the German Federal Republic (5). In general,
regulations of this kind lay down limiting values for a
series of different parameters, which must be complied with
in the drinking water.

What we must ask, however, is whether a drinking water is
really safe when the threshold values are strictly adhered
to. Do we have a sufficiently reliable and safe basis for

-------
                            -  17 -

laying down the limiting values and do, we possess the necess-
ary methods of foolproof control, especially when a large
nunber of individual limiting values has been fixed? The
answers to these questions are relatively easy and affirma-
tive in the case of the undesirable inorganic materials, e.g.
heavy metals, but it is almost impossible to specify any
limiting values when we turn to the wide range of unwanted
organics. The number of individual substances so far de-
tected analytically in drinking waters now comprises almost
1000 separate compounds, and as our analytical expertise
increases new compounds are found practically every day and
their behaviour is checked with various treatment procedures.
Although they are important and useful in individual cases,
limiting value.s alone are not enough. We neither know exactly
which individual organic compounds are present in a particular
water, nor can we establish by simple methods what toxico-
logical effects these substances may exert, especially when
it comes to cumulative effects.

On the other hand, we do need rules for the treatment of
water, we need information on the treatment plant required,
for each raw water, on the purification effects that must
be achieved, and on how the efficacy of these can be estab-
lished independently of the variations in the raw water
quality.  From this point of view it is understandable that
attempts are made from time to time to lay down rules on the
selection of treatment processes. In the formulation of the
laws and decrees it must be borne in mind, as already mentioned
earlier, that the processes used for the drinking-water treat-
ment can have not only beneficial but also undesirable effects
on the quality of the drinking water.  The following summary
shows the cause of the difficulties on some especially import-
ant examples.(Table 2)

-------
TABLE 2  UNDESIRABLE EFFECTS OP IMPERFECT USE OF PROCESSES FOR
         DRIBKING-WATER TREATMENT
Process used
Chlorination
Removal of organics
by adsorption
Flocculation
Oxidation- with
ozone
Softening
Possible undesirable effects of process if:
Efficiency too low
insufficient disinfection
residual amount of harmful
substances is too high
insufficient removal of
colloids
insufficient effect
interference by preci-
pitation of CaCO-
Treatment too extensive
formation of organo-
chloro compounds
increased tendency to ;
corrosion '
high concentration of
neutral salts ,'
germ formation in
network
increased tendency to
corrosion
increased tendency to
myocardial infarction
                                                                                     00
                                                                                     i

-------
                          -.19-

 The table  indicates  above all that, the correct dosage,  e.g.
 in oxidation processes,  is  of great significance if the final
 aim is  not only the  achievement of the process advantages but
 also the avoidance of  its disadvantages.

 'The conclusions emerging from the summary are only examples.
 In reality the  situation is considerably  more complicated,  as
 can be  seen from Table 3.

 TABLE 3 GRANULAR ACTIVATED CARBON FILTERS
ADVANTAGES
Specific removal of
dangerous organics
Good efficiency at
peak concentrations
High loadings at the
top layer
Partial regeneration
through biological deg-
radation of adsorbed
organics
Additional filtration
and turbidity removal
Easy handling and
operation
DISADVANTAGES
Not very effective for
THM removal
Chromatographic ef-
fects after longer run-
ning times
Long working zone
and partial break-
through of organics
High bacteria counts
in the effluent
Backweshing problems,
smal carbon particles
in the effluent
Difficult breakthrough
control
In this table both the many advantages and the disadvantages
of activated carbon filtration have been compiled for a report
in the USA.  Here it was intended to prescribe the details of
the use of activated carbon filters for the treatment of

-------
                          - 20 -. .

chemically polluted  river waters/ together with the basic
dimensioning and mode of operation.

While the details of this table will not be discussed further
here, it can be seen that the correct layout and operation of
a  filtration plant of this kind are determined by a whole
series of quantities and their relationships.  Only if these
are suitably taken into account will the use of activated
carbon filters be truly advantageous for the water quality.
These aspects, here only briefly outlined, are very difficult
to incorporate in a single law.  Moreover, it is not merely
a question of the raw water quality but also of the process
scheme of the treatment plant.  This applies especially to
oxidation processes.          '

Before going into greater detail another proposal should be
discussed, which is of particular significance for an evalu-
ation of drinking-water quality and which is formulated
particularly clearly in the German DIN 2000 standard (6).
TABLE 4  GENERAL CRITERIA FOR
         DRINKING WATER QUALITY
 DIN 2OOP  (Germany)

 Drinking water quality should be similar to a -

 water from the natural cycle,
 without anthropogenic impurities,
 taken from safe depth,
 after a long retention time,
 as an oxygen-containing ground water.

-------
                         - 21 -. ,

According to this, all'measurabl.e parameters of drink ing-r.  4
water quality should be oriented at the properties of a water
extracted from the natural water cycle as oxygen-containing
ground water without any anthropogenic constituents and which
can be used directly as high-quality drinking water without
additional treatment, i.e. without additional chlorination.

This type of positive characterization and orientation at a
"naturally pure" drinking w.ater enables us to draw specific
conclusions about the optimal kind of treatment in each
individual case.  This.is because, even today, there are  still
a number of ground water sources  with favourable properties
of this kind.  In addition,. ,we know the connections, processes,
and natural laws that determine the composition of natural
water of this kind, and anthropogenic influences can be
analysed well by measuring group parameters.
Viewed in this way, the-evaluation of the water quality
provides appropriate criteria for the evaluation and the
adoption of oxidation processes'. •

Aims and effects of oxidation processes
From the commencement of centralized drinking-water supply the
oxidation processes have been among the most important treat-
ment steps, since  - if performed correctly -  they guarantee
reliable disinfection and so contribute decisively to safety
of the water.  It is therefore no surprise that this aim has
very often been in the foreground of interest in the evalu-
ation of various oxidizing agents and oxidation processes,
as is still the case today.  Within the framework of this
conference, too,many reports were concerned with the problems
connected with oxidation.

However, the great significance of the disinfecting action of
oxidation processes should not obscure the fact that dis-
infection is only one of the aims in which success can be
achieved with these processes.

-------
                         - 22 -
 TABLE  5.  OBJE CTIVE S OF  OXIDAT ION'' PROCE S SES
1)   Disinfection
2)   Removal of organic constituents of water
     A)  Complete oxidation:
            4CnHm°q +  (4n  + m  ~  2q)  °2  =  4n  CO2  +  2m H2°
     B)  Partial oxidation
         with subsequent  more comprehensive purification
3)   Transformation of organic  constituents of  water e.g.
     improvement of corrosion behaviour
4)   Oxidation of inorganic undesirable materials e.g. Fe,
     Mn,
5)   Combination of objectives 1-4
 As can be seen from the table, oxidation processes play an
important part both in the removal and in the transformation
of undesirable organic and inorganic constituents of,the
water, and are now us^d successfully in many waterworks to
achieve these objectives..

In those cases where the complete removal of unoxidized sub-
stances is achieved in a subsequent process, it is of no im-
portance concerning the final quality of the final water whether
the oxidation taking place is complete, to C00 and H0O - as is
                                             ^      ^
generally the case in biological oxidation processes and with
biologically degradable substances - or whether the oxidation
is only partial - such as is the case with chemical oxidation,
especially with the use of ozone. Many such combined processes
are known today. On account of their major importance, they will
be discussed in more detail later in connection with the placing
of oxidation processes within the overall sector of drinking-
water treatment.

-------
                         - 23 -
At this point it secerns'.appropriate to consider-in  greater-v
detail an aspect to which.insufficient attention is  often
paid in the performance and evaluation of oxidation  processes.
This is the transformation of organic water constituents by  '
oxidation, mentioned under point 3 in Table 5.  In the
              /
practice of drinking water - treatment two important effects
are at work here.

In the first place, the molecular-weight distribution of the .
organic water constituents is; modified by oxidation  treatment,
here discussed on the example of ozone.  The following figure
shows some results-of such studies on the basis of experiments
with biologically purified- domestic waste water.
16
oi 12
E
o 8
O
a
0
20
16
'£ 12
c
> 8
4
0
unbehandelt
~

-
xooooo
30000-100000
10000-30000
1000 - 10000
iooooo
30000-100000
IOOOO - 30000
1000 - 10000
<1000
\
\
\\
\^v
\ \f
\ \

Fallung*
Fallung Ozonong
1gCa(OH)j/l 1gCafOH)2/
UV/DOC=0,«9 1 mg Oj /mg
UV/ OOCsO,


— .i 	

1
U
I
C
68

Ozonung
mg O3/mgC







-
/
//'
/'//
/ ^
unbehandelt
> 100000
30000 -100000
10000 - 30000
1000 - 10000
< 1000
unbehandelt
JV/DOC = 1,2
> 10OOOO
30000 - 100000
ioOOO - 30000
1000 • 10000
<1000

9

 Fig. 2  Change :qf DOC-
Change of DOC- and UV-values upon ozonation and
precipitation in biologically pretreated waste-
water (Berghausen)
unbehandelt = untreated;  Pallung = precipitation;
Fallung 4- Ozonung = precipitation + ozonation

-------
                         _  24 - -  f

The figure gives results obtained within the framework of a
project sponsored by the Federal Department for Research and
Technology on the optimization of the use of ozone, the DOC
and UV values, divided into molecular-weight fractions.  From
the values given at the extreme right and left of the figure
for the raw water it can be seen that the greater part of the
organic material in this purified waste water has a molecular
weight of under 1000.  Of course, the substances of higher
molecular weight have a higher specific UV extinction.  For
this reason, as the lower part of the figure shows, they
account for the greater proportion of the measured UV values.
Since it is precisely the materials with good UV absorption
and high molecular weight that are also very readily preci-
pitated in the form of insoluble calcium salts, precipitation
causes a strong reduction of the UV extinction/ while the
effect of precipitation on the concentration of organically
bound carbon is much weaker.

The ozone treatment, in which comparatively large quantities
of ozone were used here, in effect diminishes only the con-
centration of the lower-molecular substances.  It is mani-
fested only slightly in the UV extinction .after precipitation.
The situation with the direct action of ozone, represented
on the right-hand side, is quite different.  In this case the
decrease of the DOC is much smaller than after pretreatment
by precipitation, while the UV extinction undergoes a greater
reduction.  This"effect can only be explained if it is
assumed that in direct oxidation apart from the partial
oxidation to CO 2 and H^O, transformation of the organic water
constituents is the main process.

The relationships in the ozonization of raw water in drinking-
water treatment are very similar.  Here too we can observe,
as the following figure shows on-the.example of water from
Lake Constance,

-------
                          - 25 -
%
percentage
              Lake-Constance
              ozonation
before
I
0,1

21.1


v,
2.9
23*6 :

_±y»u


*. *>
Lake-Constance water
ozonation 68.9

0,1

50000
tooooo

r v

30000
50COO



20000
30000

8,5


after
3.4

. 10000
20000
1000
Toooo
Jo
HI.--.! 	 . Ml ...»
      molecular-weight fraction
       3   Influence of ozone on molecular-weight distribution
          of humic acid  from Lake Constance
a certain reduction of the molecular weight.  The smaller
proportions of the higher-molecular fractions and the shift
towards somewhat lower molecular substances are particularly
evident.  The molecular-weight fraction between 1000 and
10 000 increases particularly clearly, while the low-
molecular materials below 1000 undergo only a slight increase.

-------
                          - 26 -

The practical effect of this type of'- shift, in the molecular
•weight is that only a small increase of the biologically
readily degradable substances as a result of ozonization
treatment can be established, since only a few low-molecular
materials are newly formed.  On the other hand, a clear
enhancement of the adsorption of these substances on polar
surfaces is brought about by the change in the structure -
here too linked to the oxidation process - and in this case
especially by a change in the polarity of the organic water
constituents.  This is shown in the following figure on the
example of calcium carbonate.
M-l
Q)
O 1GJ
0
CO
•3
rt
M
-P
0
0)
Q.
w

4J O
•HO 10
r ^
•o
QJ Cn

njN
3
0 C
iH -H
O g
B
CH "^l*
•H tn
rt^ 1
i /
/ y
O f
d 1 /
^ i 1 /
i i 17
X ' U
I . O /
t, r
' i 1
i j
/ n
' /
/ i

A
/
* 0 g 03/m3
o 0./.S «
n 1.1 ,i
& 3.5 H


	 j___ 	 1 	 . 	 -J, 	 i 	 i 	
0 -1J 0.5 0.7 1.0 1.5 2.0 3J3
i-i (0
spectral absorption coefficient
at 254 nm in 1/m
 Fig. 4  Adsorption isotherms (Freundlich)  of substances
         in Lake Constance water on calcium carbonate
         after different dosages of ozone

-------
                          - 27 -

Fig.' 4 presents isotherms plotted for the UV-absorbing
substances in Lake Constance water.  The isotherms are
clearly shifted to the left as the addition of ozone is
increased, i.e. in the direction of better adsorption.  The
same cannot be said for the non-polar activated carbon
surface, where sometimes exactly the opposite effect is
observed.  However, it is true for polar surfaces such as
are found in the case of CaCO., or of iron oxides.

The fact that this enhancement of adsorption as a'result of
oxidation procedures is not only of academic interest but
also of practical significance is demonstrated by the
following considerations and experimental results from the
Karlsruhe Institute.
 TABLE 6  INFLUENCE OF HUMIC ACID ON THE PRECIPITATION
          OF CaCO0
Humic Acid
Lake Constance
Zurich
Haltern
Schwab. -Hall
Hann . -Fuhrberg
Without
Humic Acid
D O, C
in mg/1
0.5
0.5
1 .0
2.5
4.0
O
K
0.24
0.32
0.53
0.67
0.71
0.90
Main components
of molecular-
weight distrib.
2O.OOO - 5O.OOO
3O.OOO - 5O.OOO
1 .OOO - 1O.OOO
5OO - 1O.OOO
50O - 1O.OOO
50O - 1O.OOO
d|Ca^| _ ,
dt K Ol
Acid Number
8.0
7.4
8.0
5.5
4.6
C2+ . CQ2- _ L.
1

-------
                          -  28  -

Among other things, the change in the precipitation rate of?
CaCO., was studied for humic acids from various raw waters of
practical interest(7).After the addition of powdered limestone
the pH was artificially increased with a solution of caustic
soda.  CaCCU was precipitated from the now supersaturated
solution/ the precipitation rate being dependent on the
CaCOg supersaturation as shown in the formula in the lower
right of  the table. - The smaller the constant K, which
can be determined experimentally, the more does the humic
acid hinder the precipitation of CaCO.,.  From the numerical
values of K it can be seen that the humic acids of Lake
Constance and Lake Zurich are most effective.  However, they
have the highest mean molecular weight and as a rule higher-
molecular substances are also better adsorbed.

There are also other differences between the other three humic
acids, in spite of their approximately equal mean molecular
weight. The acid numbers are different here.  The higher  the
acid number, the lower is the polarity of the organic
material, and the better is it adsorbed.  However, the extent
of the adsorption determines the inhibition of the CaCO.,
precipitation rate and hence also the magnitude of K.

On the one hand oxidation with ozone increases the polarity
of the organic material, thereby improving its adsorbability,
but on the other hand it reduces the molecular weight and
this leads to a deterioration of the adsorbability and so to
a smaller inhibition of the CaCO., precipitation rate.

Nevertheless, with the ozone doses normally used in the
treatment of drinking water, the beneficial effects predom-
inate in most cases, as the results of the following table
show on .one example.

-------
                            29  -
  TABLE 7  K-va'lues of' CaCOa-precipitation and action numbers
           WZ.^- as a function of O-, concentration in water
             Ho                   J
           from the river Danube (WZ^., = action number humic
                                    OD
           acid)
' Treatment step
of raw water of
riv6r Danube
Flocculation
Ozonation
(2.5 mg/03)
+ flocculation
Ozonation
(5.7 mg/1 O3)
+ flocculation
Flocculation
and C-removal by
activated carbon
(F 400)
DOC
mg/1
2.75

2.32

1 .91

0.20

r in x
" """"mol.min.mg.
O.37

0.20

0.23

0.80

1° _!
W7 — ^
W"HS DOC
0.4

- 1 .3

1.3

O.O

Here the K-values were obtained after flocculation and after
the addition of two different amounts of ozone, the DOC also
having been reduced by this treatment.  The K-values were
compared with those of a water from which the organic
material had been removed almost completely by flocculation
and adsorption.
Both from the K-values themselves and from the action number
referred to the DOC it can be seen 'that after the ozone treat-
ment the organic material remaining after flocculation assumes
considerably more favourable properties as regards its action
on the inhibition of the CaCO, precipitation rate. Increasing
ozone additions cause no further improvement.  It may be
deduced from this that the values of the action number will
decrease again when the amount of ozone is increased further.

-------
                           - 30
 For the practical significance of these  results.ittis import-
 ant that completely analogous effects can  be  measured for the
 oxidation kinetics of bivalent iron and  that  this  result can
 also be extended to the corrosion behaviour of  steel pipes.
 Although it is impossible to go into details  at this point,
 a  result from field trials with Lake Constance  water can be
 shown here, from which this connection may be discerned.
 CM
  0)
  O
  3
  10
  o
  -p
  c
  o
  o
        400-
o Bodenseewasser, vp = i.sm/s    (Lake Constance water)
 Bodenseewasser nach Ozonung,  fLake Constance water
                       after ozonation)
                             300
                                   400
             oneration time in days
  Fig. 5  Iron content of  surface  layer in Lake Constance
          water before and after ozonation
The figure gives  the  amount of iron in the corrosion products
of a steel pipe at  a  flow rate of 1.5 m/sec for the almost
solids-free Lake  Constance water before and after ozonization

-------
                          -  31 —

and 'filtration.  Since the amount of the corrosion products
constitutes a measure of the corrosion rate, these results
reveal that after ozonization a transformation had taken
place in the organic material dissolved in the water of
Lake Constance, such that the corrosion rate under the given
conditions fell to a fraction of the corrosion rate with the
raw water.

If the significance of the possible beneficial transformation
of the water constituents by oxidation is to be clearly and
appropriately demonstrated,the results obtained in these
tests, close to actual practice,- are most suitable.  However,
when it is considered that this effect is in no way bound to
occur in a water with differently combined constituents, and
that the opposite effect can occur even with the water of
Lake Constance if the amount of ozone is too high, it becomes
evident how difficult it can be to asses in particular test
results of oxidation processes.  Care is1 therefore needed
when making general statements on the use of such procedures
in drinking-water treatment.  This is why so many authors
and reporters are to be found at this conference, for it is
only when the problems are viewed from various sides that it
becomes possible to deal with them fairly completely.  For
this reason the rest of this paper can only be regarded as
an introduction to the problems in question, with particular
emphasis on some aspects that to me seem of special import-
ance.

Survey of oxidation processes
The difficulties in obtaining a reliable and generally valid
survey of the advantages, disadvantages, and the signifi-
cance of individual oxidation processes in the treatment of
drinking water are not only due to the fact that with these
processes we are attempting to achieve a wide and varied
range of aims but also to the variety of possible processes,
a summary of which is provided in the following table.

-------
                          - 32  -
 TABLE 8   OXIDATION PROCESSES USED'FOR THE'TREATMENT
           OF DRINKING WATER
  CHEMICAL OXIDATION with
                                Chlorine
                                Chlorine dioxide
                                Ozone
                                Permanganate
  PHYSICO-CHEMICAL OXIDATION with
                                UV irradiation
                                 y-radiation
  BIOLOGICAL OXIDATION with
                                Slow filtration
                                Soil passage
                                Biologically working filters
Accordingly, oxidation processes can be carried out in such
a way that the oxidizing agent is added to the water,
chlorine and ozone being used most often.  The physico- '
chemical processes are of much smaller importance, only UV
irradiation being used in a few rare cases in the practice
of drinking-water treatment.

In contrast to this, biological oxidation is the mostly used.
oxidation process in Central Europe.  The technologies used
here, listed in the table, are some of the oldest methods
in the treatment of drinking water.

In the course of the conference more detailed information
will be given on the individual processes, so that in -this
introductory paper only some especially important aspects of
three of them will be considered, namely chlorination,
ozonization, and biological oxidation.
In connection with the various objectives of oxidation
processes already mentioned, it must first be said that

-------
                          - 33 -.


nearly all- • these -processes can be used.both for the oxid-
ation of organic  and inorganic water constituents and for
disinfection.   The two aims are often combined.  However,  in
recent years experience has shown that it is particularly
useful to separate these aims by the combined use of diff-
erent oxidation methods.   Moreover,  rather than using two
oxidation processes simultaneously,  e.g. ozone and UV
irradiation, it is preferable to apply two processes in
succession.  The  best known and by far the most important
example of  this is the combination of chemical and biological
oxidation,  the  particular aim of which is to convert bio-
logically resistant materials into degradable ones by chem-
ical oxidation  and so to achieve a more extensive and
efficient biological oxidation (8). Hie importance of this
procedure was first realized after it had become known that
by means of high  chlorination,  normally used previously, com-
pounds can be formed that resist biological oxidation  (9) . An
example of this is given by the  following studies in the
Donne waterworks  of Rheinisch-Westfalischen Wasserwerke in
Mulheim.
   1O
    8

    6

    4
    2
   5
_  4
£  3
8  2
a
         mit Hoehchtorung  I   I ohne Hochchtorung
         vor der Flockung  '	' vor der Flockung
       Ruhr
           nach
           Flockung
                   nach Ozonurtg
                   u. Filtration

                          nach
                          AktivkoMe
                             1
 Fig.  6
 Comparison of change of
 UV- and DOC-values at
 Dohne waterworks (RWW)
 with and without break-
 point chlorination
 mit/ohne Hochchlorung =
 with/without breakpoint
 chlorination;
 vor  der  Flockung =
 before  flocculation;
 Ruhr  = river Ruhr;
 nach  Flockung  =
 after flocculation
 nach  Ozonung u.  Filtration
 = after  ozonation and
 filtration;
nach Aktivkohle  = after
activated carbon

-------
                          - 34 -

This diagram shows the mean values with their" standard-  V"-;  '*
deviations, over a longer observation period,of the dissolved
organic carbon  (DOC) and,the  UV extinction  (254 nm) for a
method with and without break-point chlorination.  Further
treatment was in both cases the same and consisted of
flocculation with sedimentation, treatment with ozone, and
i
both gravel and a subsequent  activated-carbon filtration.

The DOC values in particular  show clearly that the high
chlorination performed before the flocculation and sediment-
ation reduces the activity of the flocculation plant and of
the activated-carbon filter.  The same is true for the
subsequent soil filtration.

These results explain, as do  many other findings, why  more
detailed attention has been paid to the processes involved
in the chlorination of water  in recent years.

Advantages and disadvantages  of chlorination
The oxidation with chlorine started to be introduced into
drinking water treatment roughly arouni the middle of the last
century though even then it was a controversial subject, and
in the last 50 years became the most commonly used oxidation
method.  This is because chlorination has some important
advantages, which are contrasted with its disadvantages in
the following table. (Table  9)

With chlorine used in the correct quantities reliable  dis-
infection is always achieved. -  It is simple to meter out the
chlorine, and the investment costs are reasonable.  Chlorin-
ation also allows the preservation of a residual chlorine
content in the distribution network.  With the oxidation of
ammonia to gaseous nitrogen,  which is possible even at low
temperatures, high chlorination also effects the removal of
unwanted material, the concentration of which has risen
strongly in the last 25 years in many effluent-loaded

-------
                         - 35 -
TABLE 9:  ADVANTAGES AND DISADVANTAGES OF CHLORINATION
                   Oxidation with Chlorine

          Advantages              Disadvantages
 Reliable disinfection

 Low investment costs
 Easy to meter out,
 simple to control
 NH.-oxidation up to
 nitrogen at breakpoint-
 chlorination

 Residual amount in
 distribution network
Occassionally large
additional amounts
required
Danger of chlorine
gas in case of
accidents
Formation of organo-
chloro compounds
which may be health
hazards
surface waters.  When we look at the historical development

of the process we find, surprisingly, that the first works

and patents on chlorination were concerned above all with

the oxidizing action of chlorine and with the possibility of

removing organic impurities in drinking-water treatment.

From this point of view we can see why already before the

turn of the century the question was asked whether it was

right to use such a strong oxidizing agent as hypochlorite

for drinking-water treatment.  It was thought that it might

be better to do without strongly contaminated raw water than

to treat it with very large amounts of chlorine and supply

this to the consumer.
 This cry for "naturally pure"  water would certainly have been

 much more urgent if what we know today as a result  of our

 considerably improve^  analytical possibilities  had  been  known

-------
                           -  36  -

then.  When  chlorination is  applied"to organically loaded
water, especially by  reaction with humic acids,  large quanti-
ties of organic  chlorine compounds are formed,  the best
known of which are  the  haloforms,  whose distribution in
drinking water in Canada according to a recent  publication
is shown in  the  following figure  (10).
10'


rH 10'
Cn
p.
fi
** 10°
d
o
•rl
4J
(d
jj 10-
§
o
o
o ,
102
10 3
__...« " *CHCI3
M
M
,CHCI2Br
H
M* ' « *
..**"' ' .•"
. • « ,CHCIBr2
* * *
x *
.'* .- 'CHBr3
«* * • «
„ » ,
.*
*
..**
* »
*
, » • . •
; .•
• .

•

.

      O.1
             5,0
                     50,0
   95.0    99.9
90.0    99.0
         1.0  ~  tO.O
       Frequency distribution in %
 Fig.  7  Frequency distribution of trihalomethane
         concentrations in treated water
         (from:  National survey for.halomethanes in
          drinking water, Canada 1977)
The frequency distributions of .the measured concentrations
shown in the figure are somewhat more  favourable  than  the
corresponding values for the United States.  However,  the
values are rather higher than the ones measured by our
Institute in Central Europe, i.e. in the German Federal
Republic and Switzerland.  Relevant information on this  is
contained in the following figure.

-------
                            37 -
 c
 o
 •H
 4J
 Iti
 M
 G
 O
 O
 O
 s
 ffl
    10

   10
     3
                         • CHCI3
                         • CHd2Br
                        '• CHCIBr2
                         •CHBr3
     0-1
       5.0
50.0
90.0 95° 99.09"
         1.0    W.O
      Frequency distribution  in  %
 Fig.
 8   Frequency distribution of trihalomethane
~~   concentrations in drinking waters in the
    Federal  Republic of Germany and Switzerland
What is particularly  important,'and it makes a general state-
ment very difficult,  is  that  even in the case of approxi-
mately similar waters the values show very large scatter,
though the causal connections cannot be clearly explained.
This is also shown by the results reproduced in the following
figure, which come from  the above-mentioned work of the
Canadian Ministry of  Health.

This figure shows the trihalomethane concentrations of
various waters in Canada in dependence on the chlorine
demand, i.e. in dependence on" the chlorine consumption. The

-------
                          - 38 -
^0-5
rH
I  0.4
   0.3
 4J
 S °-2
 •p
 P!
 0)
 O 0 1
 g
 u

     o
                     = O.127x*0.13x
                                y O.OBSx+0.04.
      0
Fig. 9
                               Values measured at
                               Canadian waterworks
                               best-fit line for Canada
                          	 best-fit line for USA
                               ace.  to,S'ymons et al.
       1.0
                        2.C        3.0        4.0
                        c' lorine demand in mg/1
                                                     5.0
Dependence of  trihalomethane formation on the
chlorine demand,  according to measurements
made in Canada and the United States
large scatter range of  the  data  can be clearly seen,  and it
is clear why similar measurements  in the USA lead to  a
different best-fit line than  the Canadian studies.
As already mentioned at the beginning  of  this   paper,  the
volatile chlorine compounds constitute only  a  small  part of
the total organic material formed during  chlorination,  as
shown in the following table.

These results were obtained with Neckar water  in the Technical
Works in Stuttgart within the framework of a BMPT research
programme.   It can be seen that the values  of the dissolved
organic chlorine  (DOC1) are greater by a  factor of 10  - 20
than the trihalomethane concentrations, and  furthermore,
that it is extremely difficult and expensive to remove  the
chlorine compounds from the water, e.g. by activated carbon,
once they have been formed.

-------
                          - 39 -
TABLE 10  EFFECTS OF CLASSICAL TREATMENT FOR A RIVER WATER
          (NECKAR,STUTTGART) WITH BREAKPOINT CHLORINATION
          AND ACTIVATED CARBON FILTERS
River After breakpoint After GAG After GAC
water chlorination,fkxxu Carbon Carbon
lation.sedimen t <5<5 f inn
tation and filtration La::5 r juu
Dissolved organic 5.O 4,1
carbon (DOC) mg/l
UV absorbance 10.9 8.6
at 254 nm m '
Sum of haloforms (jg/l 0.4 50
Total organic 33 524
chlorine (TOCI) (jg/l
3.1 1.6.
5.0 3.6
16 25
364 296
However,such measurements must not automatically  lead  to  the'
conclusion that chlorine should in general be abandoned as
an oxidizing agent.  If stepwise chlorine oxidation  is used,
in place of the break-point' chlorination common up to  now,
then from the beginning much lower concentrations of chlorine
compounds are formed and a considerably better purification
effect is obtained, particularly with' activated-carbon treat-
ment.              •           ••"•'•
Stepwise chlorination means that before the flocculation and
activated-carbon filter only so much chlorine is added, and
even then intermittently if possible, that no free chlorine
is present over a longer period.  For this purpose the
amount of chlorine must be kept well below the break-point,
as shown in the next figure.               •   •   '

For every gram of chlorine introduced considerably "lower halo-
form and DOC1 concentrations are then produced,  the reaction
time also being significant.   -          '

-------
                         -  40 -
                                        Fig. 1O
                                        Concentrations of
                                        chloroform and TOCI
                                        as a function of the
                                       .initial concentrations
                                        of chlorine after a
                                        reaction period of 2O h
This is an impressive example of the -fact that the undesir-
able effects of this oxidation stem not from the type of the
oxidizing agent itself but from the conditions of its appli-
cation.  In oxidation processes in particular there are
nearly always certain optimal doses of the oxidizing agent,
and doses exceeding this optimum .are sure to have unwanted
results.
From the point of view of naturally pure waters ,it is also
incorrect to attempt to eliminate the disadvantages of a
water containing too much organic material simply by adding
larger amounts of chlorine.  If the relationships are prop-
erly considered and the right combination of purification
methods is chosen, then in spite of the apparently great
advantages of ozonization, chlorination can and will continue
to be used successfully for the oxidation and disinfection in
drinking water treatment.

-------
                             -  41 -

Advantages and disadvantages  of oxidation with ozone
The  preceding outline of oxidation with chlorine has  shown
that the principal disadvantages of  this oxidizing agent
appear when  the chlorine is added to the raw  water before
flocculation and when the amount added is so  large that a
concentration of free chlorine is maintained  for a longer
time.   This  type of  procedure is called break-point chlor-
ination and  it is most frequently used in the classical
process scheme for the treatment of  drinking  water.   This is
shown in the next figure on the extreme left.
 breakpointchtorination    breakpointchlorination    breakpointchlorination
    flocculation
   sedimentation
     filtration
   safetychlorination
     flocculation
    sedimentation
     filtration
               activated carbon  ozone
                   filter     treatment
 safety      safety
chlorination  chlorination
                                      flocculation
 sedimentation
     I
                                       ozonation
                                       filtration
                    activated carbon
                        filter
safetychlorination
                                     ozonation
                flocculation
                                                    sedimentation
                                       t
                                     ozonation
                                     filtration
               activated carbon
                   filter
safetychlorination
    Fig.11  Process scheme development  for river-water
             treatment  in Europe (without underground
             passage)
In  Europe this simple scheme  began  to be supplemented 10
20  years ago,  in such a way that the  excess  chlorine  and
part of the  chlorine  compounds formed were removed  in an

-------
                          - 42 -

activated-carbon filter  (11). The disadvantage of this is the
rapid exhaustion of the carbon filter as regards adsorption
but not as regards dechlorination.  Therefore processes have
been favoured, especially in France, in which instead of
adsorption another oxidation is carried out, but this time
with ozone in place of the chlorine  (12). This procedure is
particularly effective in the removal of undesirable odours.
However, with many waters relatively large amounts of
chlorine are required for the subsequent safety chlorination
if a repopulation of the water with bacteria in the distri-
bution network is to be avoided.

This disadvantage is not observed in the last but one cited
process scheme, which was first used successfully in
Langenau waterworks- of the Stuttgart water supply (13) . Here
ozonization is carried out after flocculation and sediment-
ation and before filtration.  This type of process has the
advantage, if the initial amount of chlorine is not too large,
that biological processes are set up in the activated-carbon
filter, leading to a longer filter running time.

Because of the otherwise too:great disadvantages due to
break-point chlorination, this process is limited to waters
whose organic loading is not too high. For this -type of water,
e.g. as is found in the lower Ruhr in Mulheim,the use of
ozone treatment alone is more suitable, with a preliminary
ozonization before flocculation and a main ozonization
stage after sedimentation and before filtration.  A detailed
plan of this plant is given in the next figure.

The initial addition of ozone takes place in a mixing chamber
together with the addition of the flocculation agent, with
very high turbulence and a short reaction time.  This type
of pre-treatment promotes the subsequent flocculation, which
in this case is performed in a pulsator.   Then follows the
main ozonization stage and the gravel and activated-carbon

-------
                    PLOW DIAGRAM OF THE DOHNE WORKS
   RUHR
Mixing Tank
                 PA.C. Ca(OH),
                 Ozonized Air
Gasification Double- Activated
    Tank     layer   Carbon
             Filter  Filter

                       Y
                                                     Pumps
                                   Generator
   Safety chlorination

             /*
           <«8—fo-
                                                      FTTTl
                 Collecting Percolation Withdrawal  slow Filter
                    Well       Well        Well
                                                                   I

                                                                   *»
Fig.1 2  Flow scheme Dohne-Miilheim waterworks

-------
                          - 44 -,

filtration.^ tThe. mechanical, biological,- and- adsprptive <•.<••
purification in  the filters, enhanced by the  ozone, is
concluded in a soil passage - more of which later on.

This complicated but nonetheless very effective procedure
for the use of ozone as an oxidizing agent is necessary
because ozone has one disadvantage that  must  be considered,
          10    20    30    £0
           ozone consumption in ng/1
               20    30    40    50
               ozone consumption In mg/1
Fig.13  Decrease of DOC and COD in dependence
         on the ozone amount

-------
                          - 45 -'  '

Both the -GOD 'and 'the-Organic'carbon are decreased' by it.
However, even with large amounts of ozone the efficiency is
low, which is undesirable from the economic point of view.
Ozone only transforms the organic materials and by itself
cannot remove them completely.

Therefore/ an ozone oxidation within the framework of
drinking-water treatment can only be successful when one of
the effects compiled in the following  table  is aimed at.

 TABLE  11   EFFECTS OF OZONE TREATMENT
    Disinfection;
    Virus inactivation;
    Microflocculation;
    Formation of precipitable organics;
    Transformation of resistent into
    biodegradable substances;
    Promotion of bacterial growth;
    Decrease of molecular weight of organics;
    Increase of polarity of organics;
    Destruction of aromatic substances;
    Removal of hetero-atoms;
Disinfection and virus inactivation are among these effects.
Microflocculation and an improved precipitation of the organic
substances can also be attained.
Essentially more important is the improvement of biological
degradation by ozonization, described by many authors, which
is due jointly to the decrease in the molecular weight and
the increase in the polarity of the organic substances.  As
already stated, the latter also has a beneficial effect on
the corrosion behaviour.

-------
                          - 46 - ,



In contrast to .these advantages of the action mechanism there

are also the following disadvantages, shown here together

with other positive factors.
  TABLE 12  ADVANTAGES AND DISADVANTAGES, OF OZONE
            IN DRINKING-WATER TREATMENT
   Advantages
Oxidation with Ozone

                Disadvantages
  Rapid disinfection
  and virus inactivation
          High ozone consumption
          by organic substances
  No commercial chemicals
  required
          High investment- and
          operating costs
  Microflocculation
  Formation of degradable
  organic substances
          After-treatment step
          and installation required
  Increase of polarity

  Transformation of
  resistant into bio-
  degradable substances

  No formation of
  harmful substances
          Decrease of molecular weight

          Increased germ formation in
          distribution network
          Biological after-treatment
          and safety chlorination
          required
  Numerous  processes
  and installations for
  ozone input
          Difficult to control
          Mass transfer often determines
          ozonation efficiency
There are therefore cases in which particularly the raw water

has a high ozone consumption.  In such cases the already

high costs of investment and operation become really dis-

advantageous, even when no commercial chemicals are required

and no residual substances from the ozone are left over at

the end of the oxidation.

-------
                           -  47  -

 Furthermore, 'ozone can only  be  used in a few'waters  without
 some after-treatment,  and here  the problem of what processes
 and installations should be  used .to bring about the  necessary
 contact between the ozone and the  water constituents is much
 more complicated than  in the case  of the other oxidizing
 agents.  The mass transfer and  the associated relationships
 determine to a very large extent .the ozonization efficiency.
 Since this problem often receives  insufficient attention,
 although it is  of great significance for optimizing the use
 of ozone,  there are some further points that should not go
 unmentioned.

 First, selection of the correct dosage in a much more
 significant factor for ozone than  for the other oxidizing
 agents.   This is illustrated by the following figure from
 a work by Dr. Kurz of our Institute (14).

 This shows the  change in a hymatomelanic acid, i.e. a humic
 acid of  medium  molecular weight, as a function of the ozone
 addition in mg  of ozone per mg of  the acid.   The flocculation
 effectiveness was studied-.                   •         *

 It can be  seen  that small additions will primarily impair
 the flocculation efficiency,  which is ascribed,  among other
 causes,  to the  decrease of the molecular weight.

 With larger ozone additions the increase in  polarity due to
 the increased number"of carboxyl groups is  the predominant
 factor.  The  humic acid as  a  polycarboxylic  acid is thus
 converted  into  an anionic polyelectrolyte, causing a clear
 improvement of  the flocculation. .  This reaches its maximum •
 at a certain  dose of ozone,  since  from this  point on the ,
'decrease of the molecular weight, which continues  as the
 amount of  ozone added is increased/becomes the predominant
 factor.                          -

-------
                          - 48 -
                        • 5 ing acid/I I
                         10 mg ocid/j
                        • Sing acid/
                        •lOmg acid/
              2345
              mg 03/ mg acid
Fig. 14
Residual turbidity and
removal of organic
material at various
ozone dosages  (1OO mg
kaolin/1 and 5 mmole
CaCl2/l)
The flocculatibn optimum  is  fairly clearly marked in this
case.  However, it is not easy  to  achieve  this  optimum for
every composition of the  organic water  constituents.Also/the
magnitude of the ozone addition is not  the only important
factor, but also the nature  of  the addition,  as has  been
clearly demonstrated by Lienhard in recent studies at our
Institute.  To clarify these relationships some of the
results thus obtained are shown in the  next figures.

In this representation the experimental results are  plotted
on the decrease  of UV extinction  of humic acid from Lake
Constance with various doses of ozone.   The ozone was added
to the water in the .form  of  a concentrated solution, either
all at once or gradually  in  five portions.  It  is clear that
the stepwise addition has a  better effect  in lowering UV

-------
                             - 49 -

extinction if  larger amounts of ozone .are .added, while the
opposite is true for very small additions.
                Humie acid: Lake of Constance
                  a JnttrmatMit input
                  o singular ozonsinput
                        2,0    mg 03
                        	mg acid
                                       Fig. 15
                                       Spectral  absorption
                                       coefficient in de-
                                       pendence  on ozone
                                       amount, with various
                                       doses of  ozone
This effect  is even nfbre marked in the case of humic acid
from Ruhr raw water,  as can be  seen from the following
figure.
                Humic acid: Riyar Ruhr
                o singular ozori* input
                o jntsrmfttsnt azon* iiput
     0 <12 0> O.S 0,6 1,0 \2  1.4 1,6 1,8 2.0 2.2 ^^ 2f 2J
             mgOzon/mg acid
                                        Fig. 16
                                        Spectral  absorption
                                        coefficient in de-
                                        pendence  on ozone
                                        amount, with various
                                        doses of  ozone

-------
                          - 50 -

The reason for  these  differences lies in the somewhat  diff-
erent molecular-weight distributions for the two natural
humic acids.

The same effect becomes even clearer when after the ozon-
ization  a flocculation is carried out, where with rela-
tively high initial concentrations a more than 30% better
effect can be obtained in the case of the stepwise addition.
    17

    It .
  y. >
  •3. «


  S 3
              H'jmic acid; River Ruhr
              o singular ozone input
              a intermittent ozone input
    0 Of o.t 0.6 0,8 1.0 1.2 v '.6
             mg Ozon/mg acid
                            _J ___ L .,
                      1.6 2.0 2.2 2.4 2.6 2.8
Fig. 17
Spectral absorption
coefficient after
ozonation and  floccu-
lation in dependence
on the ozone cimount,
with various doses
of ozone
The changes  that occur in both cases can  also  be  seen when
the molecular-weight distribution is considered.   They are
reproduced in the next figure.

The reduction of the mean molecular weight in
both cases is apparent,  together with the fact that  the
effect is somewhat greater in the case of the  stepwise
addition.  It may therefore be assumed  that the  relative
number of carboxyHc groups is also more strongly increased  by
the stepwise  addition of ozone, and that this  is  the reason
for the better efficiency.

-------
                          -  51  -
» f " '
s
1
s«





original acid
28
15J




i

3<12

131,7


Singular ozone input
<1 ; 4
<1
3%3

S%2


intermittent
<1 V

>30000

20000
30000
ozone

-------
                          -52 -
*r"l
8
to
•s
.

CO
o
T—
Iff
3 id

J3*\
f°fe
C
la
H1-*
3 "*
O 4-5
r— I IT$
C,G
0,4


0,2



0,1
0,08
ops

Op/1

Op2
0
-
_
. »
. . 4





-u.

[



•

.
1 if1'' & Original
9 r A humic acid
A A Q Single










(Ruhr)

o 11 ozone input
/ / n Intermittent
V ' y r ozone input
mg ozone_- n _, mq ozone _ ,_
mg acM ~ /e> mg acid °'6.9
i_... • . r i i. i i i


        0
6 8 10
20
40  60
    Spectral absorption coefficient at 254 nm in m
                                           -1
 Fig.19  Adsorption  of humic acid from river Ruhr on
         CaCO3  in dependence of ozone input, with
         two different ozone doses
applications and actions  of ozone.   For example, as can be

deduced from the following  figure,  (see Fig. 2O),

the situation for an effective'virus  inactivation is more

favorable when  comparatively high, ozone concentrations are
maintained over a relatively short  time,  i.e.  when the ozone

is added not in stages but  ail 'at once.

-------
                          - .53 -
                         0,06 ppm
                                     Fig. 2O
                                     Polio virus-1'
                                     inactivation
                                     with ozone
         0,2  0,4  0,6  0,8  . 1,0
           time  in sec
In this context it should also be noted that not all types
of addition devices are suitable for maintaining certain
concentration relationships, desired in individual cases.
Precise examination of the ,discussed relationships is there-
fore very important for optimal utilization of the ozone.
In the comparative analysis of literature reports on ozone
activity too these relations must be borne in mind.  Some
of the contradictory results of trials carried out at
various places are due less to the different composition of
the organic water constituents than to the different methods
of introducing the ozone.
The fact that the influence of the material transport con-
ditions, and hence of the mode of addition, is strongly
expressed particularly in the case of ozone is due not only

-------
                           -  54  -

  to the very low ozone concentration in the gas,  i.e. in the
  oxygen or air before the passage through the ozone input system
  but is also connected with  the chemical reaction mechanisms
  known for the ozone conversion, the rates of which are
,  influenced by the ozone concentration in the water to
  different extents.  Insofar as this i-s true, studies on the
  reaction mechanism in the use of oxidation processes are of
  major practical significance.

  While in the oxidation with chlorine the true oxidation
  reactions compete with reactions leading to the  formation of
  organic chlorine compounds,  in ozonization we distinguish
  between the radical reaction on the one hand and ozonolysis
  on the other (15). Since these  two different mechanisms are also
  dependant on pH, on some inorganic water constituents such
  as bicarbonate ions, and of course also on the structure 'of
  the organic water constituents, it is "not surprising that
  very different effects of ozone treatment are observed with
  different waters (16).

  Although general regularities are difficult to formulate,
  both in the case of chlorination and in ozonization a rapid
  addition of the oxidizing agent promotes disinfection,
  because high concentrations over a short period  are required
  for this purpose.   On  the other hand,  a slow addition over
  a  longer time  has  a beneficial  effect as regards the desired
  oxidation reactions.   This  fact and the appropriate incor-
  poration of the oxidation process into the total scheme of
  treatment should be considered  if the oxidizing  agent is to
  be used to optimal effect in the  treatment of drinkin >, water.  .

  Biological oxidation processes
  The complex relationships in the  use of chemical oxidizing
  agents  indicate why biological  oxidation processes  always
  were,  and still are, preferred  in drinking-water treatment
  wherever they  are  feasible.   Here passage through soil has
  the greatest practical significance?  as the next figure shows,

-------
                           -  55- -
                           river
       riverbankf Miration
            »""•
         activated
          carbon
          filters
 ozone
treatment
                  filtration
       safetychlorination
                  activated
                  carbon
                  filters
 flocculation
sedimentation
    \
                             preozonation
                                I
                             flocculation
sedimentation
                                               ozonatkxi
                    groundwater
                    enrichment
                       I
                    pH control
                       -!
                       f
                   safetycMorination
                                                  t
                                               'filtration
                                   activated
                                   carbon
                                    filters
Fig.21  Process  scheme development for river-water
         treatment in Europe  (with underground passage)
a soil  passage can  be included  in various ways  into the over-
all treatment scheme. One possibility is the  use of river
bank  filtration as  the biological step in the process  (shown
on the  left in the  figure), combined simultaneously with the
removal of  suspended  particles   as  well as colloids, and
hence also the elimination of bacteria and viruses.  The
other possibility is  ground water enrichment, such as has
been  practised in the Ruhr or in  the dunes in Holland for
many  decades.  In this case a preliminary treatment is neces-
sary  for the more severely polluted waters, a whole series
of possibilities being available,  only two examples of which
are shown in the diagram.   •
The excellent efficiency of these  processes is  shown in the
following table on  the example of  bank filtration in the
Lower  Rhine.         .    -      .-
Here the  values averaged over 2 years are shown,  as measured
in the Hamborn waterworks of NGW.   The good purification

-------
                         - 56 -
TABLE 13  Removal efficiency of riverbank filtration
          in Duisburg (values for 1975-1976)
Mean concentration
in the River Rhine
(dissolved compounds)
Dissolved organic carbon (DOC)
Adsorbabte organics
Organic chlorine (DOCI)
Organic sulfur (DOS)
Chromium (Cr)
Copper (Cu)
Zinc (Zn)
Nickel (Ni)
Lead (Pb)
Arsenfc (As)
Mercury (Hg)
Cadmium (Cd)
Selen (Se)




6
15
90
10
9
4-
0.1
0.5
0,5
7.0mg/l
4.5 "
0.12 '
0.11 "
— 12 ug/l'
— 35 .»'
—.250 '"
— '15* ->'•>" -"
— 20 »
=-, 8 »
— O.6 « ,
— 2
2 "•
Removal efficiency
in percent

60
3O
30
20
90
70
4O
40
40
30
3O
20
0

— 75
— 50
— 40
- 30
— 95
- 80
— 60
— 60
— 60
- 60
- 60
- 50
-- 20
effect on the dissolved organic substances can be seen by the,
high efficiency of DOC reduction.  The greater part of the
heavy metals is also well retained, the data given referring
only to the dissolved metal ions.  The purification action
of bank filtration on the adsorbable organic materials is
much less favourable.  Particularly poor effects are as a•
rule observed for the organic chlorine and sulfur compounds,
and it is among these that many anthropogenic unwanted com-
pounds are found, which should not be allowed to remain in
drinking water.
The limits of purification effectiveness of biological
processes can also be seen by.considering the situation in
Holland and the infiltration-into the dunes, widely practised
there.  The following figure.shows•diagrammatically a plant
of this kind in the Amsterdam,waterworks.

-------
                         -  57  -
Fig.22   Process  scheme of dune waterworks Amsterdam
 (1)   Rhine canal
 (2)   FeClj dosing
 (3)   NaOH dosing
 (4)   Flocctilation basin
 (5)   Sedimentation basin
 (6)   Rapid filter
 (7)   Chlorine dosing
 (8)   Distribution basin
 (9)+ Percolation gullies +
(1O)   Collection canals
(11)   Collecting basin
(12)   NaOH dosing
(13 >   Aeration
(14)   Activated carbon dosage
(15)   Rapid filter
(16)   Silt deposit
(17)   Wash water reservoir
                          •i
(18)   Slow sand filter
(19)   Chlorine dosing
(2O)   Chlorine contact basin
According to this figuref in the case of heavy pollution of
the Rhine water in Holland it is necessary to perform a
flocculation^ sedimentation, and filtration before  the infil-
tration; the water also needs an additional treatment after
the infiltration, which will not be considered here in
greater detail»

-------
                          - 58 -                •   . •

The effectiveness tof.the infiltration is satisfactory as
regards the heavy metals and the ammonium but this, cannot be
said of the total organic matter present'in the water.
TABLE  14  ANALYTICAL DATA FOR THE  DUNE-WATER TREATMENT,
          AMSTERDAM 1976

DOC mg/I
Colour mgPt/I
Threshold number
Hydrocarbons mg/I
(oil)
Chloride mg/I
Bicarbonate "
Ammonia "
Nitrate- »
Phosphate "
Iron ng/l
Chromium "
Copper "
Zinc "
Cadmium •"
Mercury "
Lead "
river water
Rhine
7.1
28.O
32.0
0.26
215.O
162.0
1.5
17.0
1.2
1600.O
35.0
22.O
150.0
2.9
0.5
28.0
before after
Dune -filtration
5.4
15.O
5.0
O.O3
227.O
158.0
0.2
2O.O
0.2
100.0
<1.0
8.0
25.0
0,2
0.1
3.0
4.1
11.0
—
~ ••• •'•••
178.0
194.0
<0.05
5.5
0.1
50.0
<1.0
5.0
15.0
0.1
<0.1
3.0
drinking
water
3.6
8.
0
0
174.0
201.0
<0.05
5.8
0.1
2O.O
<1.O
5.0
10.0
O.1
<0.1
2.0
This is in part due to the fact that the fraction of .materials
that are difficult to degrade biologically is relatively high
in the Rhine in Holland.  However, it is also due ,to the fact
that the conditions for biological degradation as regards
the residence time and the medium are not quite-as good in
the dunes as on the river banks used for filtration-.

-------
                          - 59 -
From  this  point  of  view  it  is  also  understandable  that  the
processes  in which  the biological purification  is  not carried
out underground  but in industrial installations have a  limited
efficiency.  This is true above  all when slow filters are used
by themselves.   In  this  process, although really good results
^are obtained in  the uppermost  layer, the residence period in
the subsequent 1 -  2 m thick layers of sand  is  too short for
further  extensive purification.  The following  figure is a
good  example of  this phenomenon, showing the results obtained
in the infiltration of pre-purified waste water, in the  Dan
region in  Tel Avia, Israel  (17).
                        measured concentration
                    -•-  in observation well
             —O	--O-  expected concentration
             _X	x- concentration in
c
o
•H
+J
P! (H
3 en
in e
O £
O -H
«* 
-------
                            60 -
The upper part of the figure gives the purification effects
of the upper 2 m of sand as regards the COD and the perman-
ganate consumption.  It can be seen that about 25% of the
dissolved impurities are removed.

The lower figure contains the values after 3 months' residence time
underground.  In addition to"the mean values of the raw water
before infiltration and the ground water values, the figure
gives the effective numerical values measured after the soil
passage and the data that would be expected if no degradation
had taken place.

This clearly demonstrates the great advantages of a long
residence time underground.  The over-all efficiency is about
75% and the residual contents of organic substances are quite
acceptable.  It can also be seen that for part of the material
the biological degradation requires a relatively long time or
that it is incomplete.

This process is also the key to the special effectiveness of
activated-carbon filters in the biological purification of
water.  As a result of their adsorption on the activated
carbon the organic materials remain in the filter for the
long time required for the biological oxidation of a number
of substances.  The materials must of course be adsorbable,
and subsequently they have  to  be  available for  degradation,
i,e, they must be desorbable again.
The last example shows that really  astonishing,  results  can be
obtained in such filters.

This refers to trials carried out by BASF in collaboration
with our institute.  The figure shows the experimental plant,
which consisted of an activated-carbon filter through which
was circulated waste water from the BASF biological purifi-
cation plant with intermediate  aeration.   As  a  result  of this
process,  the next figure  shows, surprising purification  effects

-------
                            -  61  -
    Test-
    ar>r>aratus
     Vcfsuchskorper
                  Wattfwficf)*
            n$
                ^   Abiau*
                  *" RucMpylwSf
                  Ng.ittgjqvj emergency outlet
  outlet
                  backwash ing I water
                     ,t,r»,,.,i,cM cDntinuouli!  pH measurement
""*,TI. cbntinuou
                             -ir^r-
                                   3 oxygen measurement
              asi.  air
 Fig.24   Biological  purification plant of BASF
          (Badische Anilin- und Soda-Fabrik,
           Ludwigshafen)
of over 90% are produced with respect to the organic carbon.
The filter has been running for about 18 months without any

apparent reduction of this purification efficiency.
For the evaluation of the treatability of this waste water
in drinking-water treatment this result means that nearly

all the organic material still present in the discharge of

the.BASF clarification plant is removed without major

-------
                         - 62 -
                                  ' Fig. 25
                                   TOC-degradation in
                                   the biological
                                   purification plant
                                   of BASF  (Badische
                                   Anilin- und Soda-
                                   Fabrik, Ludwigshafen)
           A s o N D  j
            1977    I
F M A M  J J
 1978
problems in the waters and in the drinking-water treatment,
and that consequently the risk to the drinking water supply
due to wastewater is negligible.

It would be desirable if a similar testing of the waste water
constituents for the risk they present to drinking water were
also carried out in other clarification plants, and if the
results were applied to intra-works purification and waste
water treatment measures in works where there are high
proportions of organic substances which are difficult to
degrade biologically and which impede biological purification.

For the sake of completeness it should further be mentioned
that with the low loading chosen here quite similar effects
can be obtained, so that the use of activated carbon is only
advantageous here at higher loadings or still lower initial
concentrations.

-------
                         -  63 -


In any event, the example illustrates the outstanding puri-
fication capacity of natural biological processes, and the
results explain why such value is placed on processes of
this kind in Central Europe and especially here in the Rhine
catchment basin.

Nevertheless, these methods also have their disadvantages,
as can be seen from the next table.
  TABLE  15  ADVANTAGES AND  DISADVANTAGES OF  BIOLOGICAL
           PROCESSES FOR DRINKING-WATER TREATMENT
Advantages
Low operating costs
Simple operation
High efficiency
Automatic adaptation
to changed conditions
Disadvantages
Large space requirement
Small possibilities of
intervention
Susceptible to toxic
substances
Adaptation is very slow
in many cases
	
 In  this  respect  special mention  should be made of the  large
 space  requirement,  the small possibilities of intervention
 in  cases of  perturbations  and  their disadvantageous effects.
 For this reason  the drinking water supply is compelled to
 make appropriate demands on the  water protection.  From this
 point- of view  I  personally consider continuous monitoring
 of  waste-water purification with the aid of a biological
 process  such as  the- BASF operate to be more important  than
 e.g. the performance of fish tests in which acutely toxic

-------
                          - 64 -

waste-water constituents in greater concentrations,. at the
most, have  been   found.  These normally inhibit the bio-
logical purification so strongly that they can be recognized.

With these details on  the significance and application of
biological purification processes this introductory report
comes to an end.   The  considerations and results presented
in it will be amplified in many respects in the course of
the conference by means of detailed reports on individual
problems from the  sphere of the application of oxidation
processes to the treatment of drinking water.  The essential
points, which may  already be inferred from the examples and
discussion, may be summarized as follows:

Summary
1.    Oxidation processes constitute an important and often
indispensable measure  in the reliable production of safe
drinking water.

2.    A factor common  to all oxidation processes is that in
spite of their beneficial action they may also exert adverse
effects on the quality of drinking water, especially when
inappropriately applied.

3.    In this connection the formation of organic chlorine
compounds is of particular importance.  However, these dis-
advantages can be avoided if the chlorine is used suitably,
e.g. by stepwise addition,

4.    Process-technological aspects must be considered above
all if the oxidation methods are to be used to optimal effect.
This applies both to the type of the oxidizing agent addition
and to the appropriate inclusion of the process in the over-
all treatment scheme.

-------
                         _ 65 -


5.    Tlie natural biological purification processes are
currently of particular importance in obtaining unimpeachable
drinking water by the treatment of surface waters.- Their use
places certain requirements on the composition of the raw
water.  These aspects should be particularly considered in
the future treatment of waste water and be checked by suit-
able test methods.

6.    Optimal introduction of oxidation processes into the
treatment of drinking water requires an exact knowledge of
the reactions taking place and of their interrelationships.
It is the aim of this conference to review the necessary
knowledge in summarized form.

-------
                          - 66 -
(1)   ROOK,  J.J.
     Formation of Haloforms During Chlorination of Natural
     Waters
     Water  Treatm. Exam. 23 (1974) , 234-243

(2)   SYMONS,  'J.N. et al.
     Preliminary Assessment of Suspected Carcinogens in
     Drinking Water
     Interim Report &, Report to Congress USEPA, Washington,
     June 1975,  December (1975)

(3)   SANDER,  R., KtJHN, W.,  SONTHEIMER, H.
     Untersuchungen zur Umsetzung von Chlor mit Huminsub-
     stanzen
     Z.  f.  Wasser- und Abwasser-Forschung 1O (1977), 5, 155-16O

(4)   -
     Save Drinking Water Act,  Public Law 93-523, July 9 (1976)

(5)   -
     Verordnung  xiber Trinkwasser und tiber Brauchwasser fur
     Lebensmittelbetriebe (Trinkwasser-Verordnungj  31 Jan. 1975
     Bundesgesetzblatt (1975), I, 453-461, 679

(6)   DIN 2000
     Zentrale Trinkwasserversorgung
     Leitsatze fur Anforderungen an Trinkwasser, Planung,
     Bau und Betrieb der Anlagen
     Deutsche Normen DK 628.1.O33 Nov. 1973

(7)   RUDEK, R.,  SONTHEIMER, H.
     EinfluB  natiirlicher Wasserinhaltsstoffe auf die Ausfalls-
     geschwindigkeit von CaCO$
     Vora Wasser  £7 (1976),  421-431

(8)   SONTHEIMER, H., WO'LFEL, P.
     Amelioration de la degradation biologique des eaux
     residuaires par un traitement a I1ozone
     Tagungsbericht: Internationaler Ozon-Kongress Paris
     4-6 Mai  1977

(9)   SONTHEIMER, H., HEILKER,  E., JEKEL, R.M., NOLTE, H.,
     VOLLMER, F.
     The Miilheim Process
     J.  AWWA  70  (1978), 7,  393-396

-------
                         -• 67 - -
(10)
      National Survey for Halomethanes in Drinking Water
      77-EHD-9
      Information,Service, Department of National Health and
      Welfare, Brooke.Claxton Building, Ottawa.K 1 A, OK 9

(11)   HOPF,  W.
      Versuche mit Aktivkohle zur Aufbereitung des Diissel-
      dorfer Trinkwassers -
      gwf-Wasser/Abwasser 101 (1966), 14, 330-336

(12)   GOMELLA, C,                -
      OEone  Practices in France
      J.  AWWA 6j4  (1972), 39-45

(13)   WURSTER, E.,  WERNER, G.
      Die Leipheimer Versuche zur Aufbereitung von Donau-
      wasser
      gwf-Wasser/Abwasser 112 (1974), 2, 89

(14-)   KURE,  R.
      Unters.uchungen zur Wirkung von Ozon auf Flockungs-
      vorgange
      Dissertation, Fakultat fur Chemieingenieurwesen,
      Universitat Karlsruhe  1977

(15)   HOIGNE, J., BADER, H.
      Kinetik und Selektivitat der Ozonung organischer Stoffe
      im  Trinkwasser
      Internat. Symposium Ozon und Wasser Berlin, April 1977

(16)   HOIGNE, J., BADER, H.
      Beeinflussung der Oxida'tionswirkung 'von Ozon und
      OH-Radikalen durch Carbonat
      Vora Wasser  £8 (1977),  283-3O4

(17)   IDELOVITCH, E.  et al.
      Groundwater Recharge with Municipal Effluent
      Dan Region  Sewage Reclamation Project,  Annual Report 1977

-------
                           - 68  -  -

HYGIENIC SIGNIFICANCE OF CHLORINATION AN  THE REQUIREMENTS
THEREFROM                   . •   •   • ;       /
G. Mttller
More than 200 years have now passed since the discovery of
chlorine in 1774, and during all this time it has lost none
of its importance.  In earlier days attempts were often made
to bind "foul effluvium" called miasma, by fumigation and so
render it harmless, and shortly after its discovery chlorine
gas was used in France as a "fumigating agent for this purpose
(1).  Suitable means of disinfection were only developed
after the discovery of pathogens and after the infection
chain typical or specific for the-'spread of infections
became known.  In ignorance of the facts, the spread of
epidemics, e.g. of cholera and "typhus, via drinking water
was allowed to continue up to the end of the nineteenth
century.  This was due to drinking untreated surface water
contaminated by human and animal waste and, after the dis-
covery of sewerage, by effluents.  In the United States
chlorination of drinking water was used as a means of epidemic
control as early as the beginning of this century, and there-
after a sudden fall e.g. in the mortality from typhus was
achieved.

In Germany, on the other hand, in the middle of the nineteenth,
century, following Lindley's example in Great Britain, the
biological method of purification of impure river water had
been adopted and slow sand-filters were built, also bringing
about an abrupt reduction in the incidence of typhus.  The
efficacy of this measure was made particularly clear during
the great Hamburg cholera epidemic in 1892.  As is well known,
within 2 months 17,000 people were taken ill in Hamburg,
which was supplied by unpurified water from the Elbe, and
9000 of them died.  In neighbouring "Altona, where the Elbe
likewise supplied the drinking water, the slow sand-filters

-------
                            -. 69 r-


built there  kept the number'of victims down to about 200, and
these became ill not by drinking  the water but by direct
contact with the sick  (Fig.  1).   When the news of the
successful use of chlorinated  drinking water  in  the-"control
of epidemics first reached Germany from the USA  in 1910  (2),
the proqess  met Vrith opposition.   Strong objections were
voiced against chemical treatment of water and an "arti-
ficially  performed, simplified disinfection"  of  this kind.
Attempts  were made to hang on  to  the old principle that
"prevention  is better than cure", and raw water  of unim-
peachable origin was demanded, which would then  be treated
to become drinking water.  Following the discovery of patho-
genic microorganisms and .the realization of the  connection
between drinking water from .river water and drinking water
epidemics, the use of ground water as a drinking water
supply was started early onr since, when the upper soil
layers are intact and the soil is of sufficient  depth,the
biological purification processes taking place in the ground
           Cholera . Epldtml*
               Hamburg »«

           	.ErkrankungsfMile
           —JCases of disease
             Cas de maladies
           ^—Todesfalle'
           Hiverininal cases
             Cas de deces
             Vcrsorgungsgebieit der Hasserwerke von:
             Water supply arfea:' - " . " -  -•
             Region de distribution des usines d'eau;
                 Homtari
                 Mlana
   n. it is i  i tt M ;/, it t t tt ft a.
   August  September'Ofetober
   aout   seotembre octobre
  Fig.  1   Incidence of  disease and deaths  in the supply regions
         ,  of Hamburg and Altona during the cholera: epidemic

-------
                          - 70 -


ensure a reliable protection against epidemics even without
chlorination.  Today this development strikes us as being
retrograde.  The way forced upon us —  a return to surface
water  -  is in most cases, when we consider the purifying
action of the soil, taken as a short-cut, since the surface
water after passing through soil, serves to enrich ground
water.  In this way it is still possible in Germany to draw
over 90% of the water used for public supplies from genuine
or enriched ground water.  The direct fight with the patho-
gens present in unpurified surface water ceases to exist,
these pathogens being further introduced via waste water,
since regrettably even a well-dimensioned, mechanical and
biologically working purification plant is not capable of
eliminating pathogens in the purification of waste water,
with the result that bacteria are still found in the treated
water  (3 - 5).   A biological purification of waste water is
therefore not a disinfection process per se.  While cholera
epidemics connected with drinking water no longer occur in
Germany, it is only because the necessary bacteria are
absent; it has so far proved impossible to prevent the
incidence of typhus and paratyphus B epidemics related to
the water.  After the use of untreated river water as
drinking water had been discontinued towards the end of the
nineteenth century, these epidemics were caused exclusively
by short-circuits between drinking water and waste water or
between drinking water and effluent-contaminated river water.

However, in contrast to the epidemic outbreaks of the
previous century, as a result of a technically faultless
drinking water supply and its careful monitoring and control,
short-circuits of this kind are genuine "accidents".  Cases
of typhus, paratyphus B, or Salmonella infections caused by
such short-circuits ari~e very rarely directly from drinking
the polluted water; an enrichment of the few bacteria present
in the water must first take place in'the food for the
numbers necessary for infection to be reached.  This was the

-------
                          - 71 -

case in the typhus epidemic in Hagen in 1955  (infection from
milk cans) and the epidemic on a ship in Hamburg harbour in
1969, where water from the Elbe was used to wash dishes (7).
Even within the framework of legal specifications chlorinated
drinking water cannot as a rule guarantee true disinfection
in the case of cross-connections with waste water or surface
water, i.e. "putting an object into such a state that it can
                                                        *.
no longer infect" (8), since the organic substance introduced
together with the impure water is usually sufficient to con-
sume the chlorine before the latter can expect its dis-
infecting action.

Chlorine can be used in the disinfection of drinking water
for the prevention and control of hygienically undesirable
states, but it must be clearly understood that although the
chlorination performed as so-called safety chlorination can
achieve the cosmetic effect of decreasing the colony count,
it hardly ever destroys pathogens after the penetration of
waste water or river water into the supply system.  For use
^as a true disinfection agent, essentially higher concentrations
of chlorine are necessary than are laid down  in the laws on
drinking water and drinking water treatment.  This is put
into practice, for example, in the disinfection of new parts
of the distribution network, as was also done during the
1962 Hamburg flood disaster (9), when it was  only the aimed
use of large quantities of chlorine that made it possible
for about 1 million m  of Elbe water that had broken into
the distribution network, and also for the pipeline system
so contaminated, to be made bacteriologically safe.  As a
result of these aimed disinfection measures,  no increase in
the incidence of typhus, paratyphus B, and other salmonelloses
was recorded  (10).

In Germany there are many public water supply systems in
which the drinking water entering the network is not chlor-
inated, and yet the danger of epidemics is no greater than

-------
                          - 72 - •'               '    ,

                                               t ' •-   V ' i •  - t -
there is from chlorinated drinking water. 'The chlorination
of drinking water in Germany is practised for other reasons —
either to destroy the odour and taste of organic substances
during the treatment or to prevent the proliferation of auto-
chthonous aquatic flora when the appropriate nutrient media
are present in the distribution network, especially in the
case of long-distance pipelines, to keep to the legally
prescribed limiting colony counts.

In spite of the relatively restricted use of chlorine in
German public water supplies, the incidence of typhus and para-
typhus B has fallen in the last 15 years, while, independently
of this, the incidence of salmonelloses has risen  (6).   The
cause of this does not lie in chlorinated or unchlorinated
water but in the sector of food -and veterinary hygiene
(Fig. 2}.                    ...
Erkrankur
30000-
25000-
20000-
15000-
raooo-
5QQO-
1000-
500-
0-
igsjfiUe'M, «?
• Typh
D Solrr
B Hepi


1
1
1
1











c*
U
0
it

|
:
-
1
3
MI/CAS re
s/Paral
nellose
Jtis
=
E
|
=
=
-
1M.UIIC9
iyphus B
=
" =
=
=
i
-
3 H













:


-


'


=

=
z
-
=
q



i
If :
w
n E




—



i!
1 1
- •
= R
: •
|

1


=
=



-[

1
:
=

=


!•
,
i
i
= i
i
=
i
=




i
1
= 1







1962 66 67 68 69 70 71 72 73 74 75 76 year
  Fig. 2  Cases of typhus/paratyphus B, salmonellosis, and
          hepatitis in West Germany during 1962-1976

-------
                           -  73 .-

Whereas up to the end of the last century epidemics in a ,
population were mostly,of infectious origin, today diseases
of noninfectious genesis are in the foreground, the most
frequent being diabetes, rheumatism, cardiovascular disorders,
hypertension, and cancer.  In the search for the primary
causes, the environmental factors must not be dismissed.
Besides the air and foodstuffs, water should also play a part
in this.  The causal connection between water and disease was
established in the majority of cases on the basis of stat-
istics.  A connection between the constituents of drinking
water and cardiovascular diseases (11), and between these
constituents and cancer, ha.s therefore been obtained on the
basis of statistical evidence, and certain carcinogenic
substances have been identified (12).  Thus, some chlorinated
organic compounds (halogenated hydrocarbons), some of which
had only been formed in the water as a result of chlorin-
ation, have been indicated as potentially carcinogenic. •
This is a recent finding and interferes with the fact that
chlorine maintained its preferred position as a drinking
water disinfection agent precisely'because it was cheap,
easy to meter out, easy to, detect, and up to now regarded as
harmless (13) .               •;   '

In contrast to other countries, in which even the waste water
has been chlorinated for years, this measure has not been
adopted in Germany.           -   '.

The occurrence of halogenated hydrocarbons in water is there-
fore due in Germany to the" input of industrial effluents
rather than, as is the rule,;in ^America, to chlorinated domes-
tic  sewage.  Since in America-the cycle: (input of chlorin-
ated sewage into the river/removal for the production of
drinking water with preliminary chlorination, active carbon
filtration, and post-chlorination before distribution into
the network/production of waste water/waste water chlorin-
ation etc.) is relatively short and the accumulation of the

-------
                           - 74  -


halogenated hydrocarbons takes place relatively quickly/ the
findings there are orders of magnitude different than in
Germany, especially since here the probability of the form-
ation of chlorinated substances in high concentrations by
'chlorination of drinking water within the legal limits during
the water distribution is relatively Ipw.

According to American investigations (14), some of the incidence
of cancer of the stomach, intestine, and liver may be related to
trihalomethane exposure,  especially chloroform.  According to
German mortality statistics (15) on the number of deaths from
cancer of the stomach and of the intestinal tract (Fig..  3), a
rise in the general cancer mortality rate is found from 1932
to 1974, however, a fall  in the death rate from 'cancer of the
stomach is observed, and  the number of terminal cases of cancer
of the intestinal tract is fairly constant.   In a comparison of
the numbers of death from cancer of the liver, stomach,  and the
urinary tract, types of cancer which, in animal experiments
can be induced by chloroform, it -can be seen that, within a
period of 10 years  (1956 -  1967)  (Fig. 4), the deaths from
stomach cancer have decreased, those from cancer of the
liver have remained fairly  constant, while those from cancer
of the urinary tract have risen by about 25%.  Epidemio-
logical enquiries on this are not yet available in Germany.

The example of chlorination of drinking water shows clearly
how problems of hygiene can shift in the course of a century.
A measure that contributed  essentially to the control and
prevention of epidemically  occurring, infectious diseases
may now be a contributory cause of the occurrence of non-
infectious diseases.  The long years of success must now of
necessity be weighed against considerations of a completely
different kind, particularly since very little is still known
about the causes of cancer  due to exogenous noxae.  The
fact that we now possess mature and almost complete knowledge

-------
                               -  75  -
                        • Krebs. Ollgemein/cANciiu GENERAL/ CAHCERV

D
TodesffiUe/lio, OF DEATHS/CAS BE Dfcces
                                        . / STOMACH/ 1 NTiST t NAL CANCER
                                              E L'ESTOIAC/CAMCER EE L'INTESTIH
                                          CANCR/CANCER DE L'ESTOMAC
•«tf
200-
150-
100
50-

n


.




-




s
s
as
3
B
sa
i












-




1

i
i -







C
1932 35 38 50


















«








-i








-,




I
I -







1
m
55 60 65 70 % year
Fig» -3  Mortality  due  to  cancer of  the stomach  and  the
          intestine  in West Germany  from 1932  to  1974
                   g KrebS dK Homlr8MiS/e4HCER-eF THE USINMV TRACT/CANCER DE i.*App«Reji. uRowine


                   [J LfibBflcrBbS/CAHCgR OF ras mVER/CAIKER DU F<3!E


                            /CANCER 9F 1M~STOHACH/CAKCER DE t'ESTflMW;
                                                               iaaf
                                                             year
Fig.  4  Deaths  due  to  cancer  of  the stomach,  liver,  and
          urinary tract  in  West Germany  over 1O years
           (1956-1967)

-------
                          - 76 -


of the epidemic pathogens stands in opposition to the d if fir-;•/"
cult work necessary before we can make equally precise state-
ments on the aetiology of cancer.  This will be an essential
task for the twentieth and twenty-first century, inductive
epidemiology, i.e. purely empirical collection of facts and
observations/ playing at first a leading role.

On the basis of this inductive aetiological comprehension of
environmental noxae, a series of carcinogenically acting
substances has already been established.  Since water is
drunk every day, it is obvious that its constituents should
also be included in these inductive-epidemiological investi-
gations.  However, it must be understood that an evaluation
of such correlations, which have only been established
statistically, is extremely difficult, since there is no
doubt that cancer is rarely due to a single factor.  On the
contrary, many factors should be included in the evaluation,
e.g. the nature and action of the carcinogent dose, cumu-
lation, metabolism, synergistic effects, intervention or
co-carcinogenesis.  The social situation of the patient also
plays a part as an exogenous factor, e.g. occupation,
nutrition, place of residence, living habits, disposition,
and the nature and duration of the exposure to the carcinogen.
Moreover, it is difficult to say whether, and under what
conditions, an experimentally determined or suspected carcino-
genesis becomes relevant for the whole population or for a
section of the population (16), especially as the list of
the known environmental carcinogens is already very long and
is certainly still growing.   Attention should also be paid
to the ratio of the amount occurring in the environment,
established by modern trace analysis,  to the relatively high
doses used in experiments on animals.   In spite of this,  it
cannot be safely excluded that life-long exposure of people
to one or more carcinogens may lead to a cumulation of sub-
threshold doses, or that an additive effect can occur,
leading to a carcinoma.

-------
                         - 77 -



As long' 'as- 
-------
                          _ 78 -
 (9)   MOLL1R,  G.
      Welche Konseguenzen ergeben sich a'us den Erfahrungen der
      Hamburger Flutkatastrophe fur die hygienische Trinkwasser-
      untersuchung?
      Arch. Hyg.  148 (1964)  321-327

(1O)   DROBEK,  Wf.
      Auswirkungen der Flutkatastrophe auf die Wasserversorgung
      in Hamburg
      VDI-Zeitschr.  1O4 (1962), 32, 1649


 (11)  MtiLLER, G.
      Probleme der epidemiologischen Beurteilung von Wasser-
      inhaltsstoffen
      Schriftenreihe Verein Wasser-, Boden- und Lufthygiene e.V.
      Fischer ¥erlag, Stuttgart _4O  (1973)

 (12)  ALAVANJA, M./GOLSTEIN,  I., SUSSER, M.
      A case control study -of  gastrointestinal and urinary      .  .
      tract cancer in relation to drinking water chlorination
      Water Chlorination, Environmental Impact and Health
      Effects, Ann Arbor Science 2_  (1978)

 (13)  PFEIFFER, E.H.
      Ober die Schadwirkung der Wasserchlorung unter besonderer
      Berilcksichtigung der Frage der Co-Cancerdgenitat von Chlor
      Zbl. Hyg. Bakt., I. Abt. Orig. B  166  (1978), 169-184

 (14)  -
      New Orleans Area Water Supply Study
      Report EPA - 906/1O-74-OO2, Dallas/Texas (1975)

 (15)  -
      Das Gesundheitswesen der Bundesrepublik Deutschland
      Hrsg. Bundesminister fur Jugend, Familie und Gesundheit
      W. Kohlhammer Verlag Stuttgart/Mainz ]_ - 4_ (197O)

 (16)  KLAND,  M.J.
      A priori predictive methods of assessing health effects
      of potential chemical toxicants and suspect carcinogens
      Water Chlorination, Environmental Impact and Health Effects,
      Ann Arbor Science 2 (1978)

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                          - 79 -


REACTION OF CHLORINE WITH INORGANIC CONSTITUENTS OF WATER
K.-E. Quentin and D. Weil
Although chlorination is performed in water treatment for
bacteriological and hygienic reasons, the oxidizing prop-
erties of chlorine are also of process-technological signi-
ficance, for example for the precipitation of iron and
manganese and for the removal of sulphide sulphur.  Other
elements, in contrast, can pass into their stable oxidation
states and remain in the water.  An example of this is
selenium, for which,on account of its toxicityr a low per-
missible level in drinking water, in the microgram range,
has been laid down in most countries.  The oxidation
potential of oxygen-containing water is about 600 - 800 raV,
and the selenium is then present in the neutral pH region as
selenite or hydrogen selenite.  The oxidation potential is
raised by chlorine to about 1100 - 1200 mV, i.e. into the
stability region of the selenate (Fig. 1).  The relation-
ships for surface waters which are low in oxygen or organ-
ically loaded are even more remarkable.  According to our
own work on nine rivers and lakes in West Germany, selenium
is present partly in the elementary form, and this increases
with increasing content of organic matter in the water.  At
total selenium contents of 0.6 - 2.4 ug/1 the elementary
selenium ranges between O.25 and 1.9 yg/1, corresponding to
ratios of elementary Se to SeO^~ of between 1:1 and 4:1
(cf. Table 1).  While the elementary selenium may be elimin-
ated by flocculation, this is not so effective in the case
of the selenite or selenate.  Arsenic, on the other hand,
with consideration of the usual oxidation potentials of raw
waters, is from the outset present predominantly as arsenite
or arsenate  (Fig. 2).  It also appears necessary to pay
greater attention to these effects of chlorination in the
future, since we have no detailed information on the occurr-
ence and the behaviour of individual inorganic species of

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                          - 80 -

such elements, especially as regards the course and the conse-
quences of oxidative water-treatment processes.
TABLE 1   Selenium content in West German waters ug/1 Se (1977)
Waters
Danube
Rhine
Lake
Constance
Main
Ruhr
Pulda
Weser
Elbe
Neckar
Collection
site
Leipheim
Passau
Basel
Cologne
Duisburg
Karlsruhe
Sipplingen
Frankfurt
Miilheim
Kassel
Bremen
Hamburg
Ludwigsburg
Total
selenium
1 ,40
1,10,
1,20
1 ,90
2,OO,
1 ,20
0,6O
2,40
1,90
1,45
1 ,60
2,20
2,30
Selenite
O,25
O,,5O
0,65
O,6O
0,70
O,7O
O,35
O,5O
O,50 .
O,45
0,35
0,75
O,5O
Elementary
selenium
1,15
O,6O
0,55
1 ,3O
1,30
0,50
0,25
1 ,9O
1 ,4O
1 ,OO
1 ,25
1,45
1,80

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                       -  81 -
Fig. 2
Stability regions for
As species.
    1O~ M = 75 pg/1
                               Fig.  1
                               Stability  regions  for
                               Se  species.
                               unbelastete  C>2haltige  WMsser  =
                               non-loaded waters  containing  O2;

                               belastete  Wasser = loaded waters

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                           - 82 -


 More important by far for the treatment of water, however,
 are the reactions between chlorine and ammonia or ammonium
 ions.  These reactions are at the basis of technologies
 whose application and further development are of topical
' significance.  The technological aim is on the one hand the
 degradation of nitrogen by a so-called break-point chlorin-
 ation in which elementary nitrogen is formed as the main
 product; organic nitrogen compounds are also partly involved
 in these processes.  In the second place the formation, of
 monochloroamine is intentionally brought about, to maintain
 a long-term germicidal action in extensive water-distribution
 systems'.  Chloramine is also preferred when certain substances
 in the water, such as phenol (1) , form with the chlorine
 substances having undesirable odours and tastes (chlorophenols)
 The formation of these is substantially retarded by the pres-
 ence of ammonia in the water, owing to the latter 's fast
 reaction with chlorine.

 When chlorine is added to waterr hypochlorous acid is produced
 by hydrolysis, which dissociates to the hypochlorite ion
 according to the pH and the buffer capacity of the water. If
 ammonia is present, mono-, di-,  and trichloroamine are then
 formed :

                 NH  + HOC1  ^   -  NH_C1  +  H0O
                    j                  £        £
              NH2C1   + HOC1  ^   ~-  NHC12  +  H^O
              NHC1   +  HOC1 .,     NC1-,    +  H0O
                  *-•                    _J        <£
 Monochloroamine is produced first in a second-order reaction
 (2),  i.e.  in dependence on the concentrations of both re-
 action partners.   On the basis of kinetic  investigations a
 molecular  mechanism is  assumed for this reaction,  i.e.  the
 reaction is  dependent on the pH and hence  on the dissociation
 equilibria of ammonia and hypochlorous acid.  In contrast to

-------
                         - 83 -
the case of monochloroamine formation, the rate constant for
the chlorination to dichloroamine as a non-catalysed reaction
                                          4
of second order is lower by a factor of 10 , because NH2C1
is less nucleophilic in .^comparison with NH.,.

In various studies it was further established that the
formation of dichloroamine is associated with acid catalysis
via the pH and that disproportionation of monochloroamine
also plays a part in this reaction.  In addition to direct
chlorination of the monochloroamine at the nitrogen by a
second molecule:
            2NH2C1  -  ~~ NH    +  NHC12
another two-stage mechanism can also take place:

           NH2C1  +  H20 ===== NH3  +   HOC1

           NH2C1  +
This comprises a slow first-order hydrolysis followed by a
fast formation of dichloroamine.  Any ammonia or ammonium
present competes with the monochloroamine and exerts a
stabilizing effect both on the monochloroamine and the
dichloroamine.

Little information is available on the kinetics of the tri-
chloroamine formation, but it may be assumed that the uncat-
alysed chlorination proceeds very slowly to the third stage.
The optimal pH is below 4; at higher pH the chlorine must
be used in excess.  To illustrate the course of the reactions
of chlorine with ammonia, knowledge of which is of consider-
able interest to the treatment of drinking water, universal
break-point diagrams were constructed in three-dimensional
representation (3) (Fig. 3) .  The formation and degradation
of chloroamines in dependence on the chlorine addition can be

-------
                         -  84  -
                                HOCI.OCI-
Fig. 3  Universal break-point  diagrams
        nach  ... Reaktionszeit =  after  ...  of reaction time;
        Chlorbindungsvermogen   =  chlorine-binding capacity;
        Chlorzugabe  in mg/1...  =  chlorine addition in mg/1
                                  to O.5 mg/1 N in (NH3 or
                       Einheit =  ijnit;

-------
                          - 85 -

seen from the diagrams;  the  break-point  formation  after
various action times at pH 4  -  9  is shown in addition.  The
stability regions for hypochlorous acid and hypochlorite  and
for the individual chloroamines,can be represented,  as  for
selenium and arsenic  (3,4)  (Fig,  4).
Uj
    456759
                              Fig. 4
                              Stability regions for HOCl/OCl"
                              and chloroamines;
                              Concentration range
                              5 x 10~ M"»0.36 mg/1 C12
Following the addition of chlorine to a water containing
ammonia or ammonium  ions .(or the other way  round),  the
following pattern is observed:  at a pH of  about  7  and
higher monochloroamine is formed; below this pH the form-
ation of dichloroamine and trichloroamine is preferred.

-------
                          - 86 -

Break-point'formation is also impaired, because the excess
of HOC1 contributes to a stabilization of trichloroamine;
the presence of trichloroamine  in water is undesirable on
account of its irritating action.  The monochloroamine—
dichloroamine ratio is determined mainly by the pH and less
by the excess of ammonia.  However, an excess of ammonia
slows down the degradation of mono- and dichloroamine.

The degradation reactions of the nitrogen compounds proceed
most rapidly in the region of pH 7.  Monochloroamine, which
is relatively stable in the presence 'of ammonia, is degraded
by an excess of chlorine:
      2 NH2C1  +   HOC1—N2 +  H20 + 3 H+ -t- 3
However, this over-all equation does not provide any insight
into the mechanism.  It has been found (5) that the break-
point formation is essentially influenced by the inter-
mediate occurrence of dichloroamine, which is in turn
decomposed via nitroxyl radicals with an increase of free
chlorine to nitrogen as the main product  (partly also to
nitrate) .
       2 NHC12   +  H20 - - - N2  + 3 H  + 3 Cl' + HOC1
The chlorine-nitrogen ratio is most favourable in the region
of pH 7; at 0.5 mg/1 N as NH3 or NH.  in the water this
ratio is 8.2:1 by weight or 1.64 moles per mole.  This ratio
changes slightly to the chlorine side at higher pH but
strongly at lower pH, i.e. more chlorine is used up; more-
over, a lower pH is also undesirable because the desired
break-point formation is then not attained; only a partial
degradation of nitrogen compounds occurs.  The nitrogen
remains in the water for a longer time,  largely as dichloro-
amine and trichloroamine.

-------
                          -  87  -

Since most natural waters have a neutral pH, reactions of
this kind are not immediately expected.  However, during
water treatment^the situation can change.  Relatively small
shifts of pH in the course of the treatment, e.g. due to
loss of CO.-, as a result of a low and concentration-determined
                           —    2 —
buffer capacity of the HCCU /C03   system, affect the form-
ation and degradation of chloroamines and thus the break-
point development.  The temperature of the wate-r is also
among such effects, because these reactions proceed more
slowly at lower temperatures.  However, a concentration
shift also occurs in the chlorine-nitrogen compounds formed.
The amount of chlorine for the oxidation of ammonia becomes
somewhat smaller; .also, an increase in ammonia (e.g. from
1 to 1.5 mg/1 N) leads to a lower relative chlorine con-
sumption for the oxidation; the reason for this lies in an
intensified nitrate formation at lower ammonia concentrations
in the water (5).

All this indicates that, like other oxidizing water treat-
ments, chlorination can exert certain effects on the inor-
ganic water constituents, effects that must be investigated
in greater detail in the light of present-day water loadings
and the legal specifications.  The principles of chloroamine
formation,which is both an essential reaction of chlorine
with ammonia and an important water-treatment process,
should also be established.  Since it is precisely here that
we find that the course of the reaction can differ according
to the type of the water, appropriate preliminary studies on
the practical performance of the process must be carried out
in each individual case.                                    /

-------
                         _ Q Q
                           OO ""
(1)   BURTSCHELL,, R .M.,,, ROSEN , .A.A.,; ''IDDLETON, F.M*-,
     ETTINGER,  M.B.
     Chlorine derivatives of phenol causing taste and odor
     J.  AWWA J5J_ (1959) , 2, 205-214

(2)   MORRIS, J.C.
     Kinetics of reactions between aqueous chlorine and
     nitrogen compounds.  principles and applications of
     water chemistry
     Proceedings of  the fourth Rudolfs Research Conference
     John Wiley &  Sons  Inc., New Yprk/London/Sydney (1967)

(3)   WEIL, D.,  QUENTIN, K.-E, "  '•"  '
     Bildung und Wirkungsweise der, Chloramine bei der
     Trinkwasseraufbereitung
     Z.  f. Wasser- und  Abwasser-Forschung _8 (1975), 1, 5-16

(4)   POORBAIX,  M.
     Atlas of electrochemical equilibria in aqueous solutions
     Pergamon Press, London (,t966)

(5)   WEI, I.W., MORRIS, J.C.
     Dynamics of breakpoint chlorination
     Chemistry  of Water Supply,  Treatment and Distribution,
     Ann Arbor  Science  Publishers Inc., Michigan (1974)

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                         - 89 -
CHLORINATION - 'PRACTICAL- REQUIREMENTS FOR ITS' APPLICATION*
Y. Richard                    , '   .


Introduction              ,   "•  :
The main reason why chlorine is, used in the treatment of
drinking water is to allow adequate disinfection.  Origin-
ally, this disinfection was carried out at the end of the
treatment line, just before pumping of the water into the
distribution network.         •  •,

It is the aim of this report -to examine not disinfection
proper but the phenomena that occur in disinfection when
chlorine is introduced into the water, and during the contact
time corresponding to passage of the water through the dis-
tribution network.

On the other hand, only the case of chlorine will be con-
sidered, the other oxidizing agents having been studied
elsewhere, though the possible interference of these diff-
erent oxidants will be alluded to.

Once the water has entered the network, there is theoret-
ically no further chance for the pathogenic bacteria elim-
inated in the disinfection treatment to develop.  Neverthe-
less, experience shows that there is a risk of contamination
in various parts of the network,- namely in pipe couplings,
valves, and reservoirs.
            I
The disinfectant will therefore need to reduce the risk of
possible contamination, and the application method will
either'have to lead to an effect of persistent disinfection,
or cause a residual amount of disinfectant to be maintained
in the network.  It is the latter requirement that is applied
in chlorination.

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                         -  90 -


Wo shall therefore try to determine the conditions of the  •
use of chlorine allowing a residual amount of chlorine to be
maintained  in  the distribution network.  The concentration
of the residual chlorine should furthermore be as constant
as possible, so that  it can be the  same whatever the con-
sumption situation in the distribution network.

I.       Determination of the chlorine demand of a water
Two  tests are  needed  to obtain an idea of the chlorine demand
of a water.  The first of these is  the absorption curve of
chlorine,which allows a determination of the amount needed
to obtain a residue of free active  chlorine  (critical point);
the  second  is  a behaviour test allowing a determination of
the  evolution  of the  chlorine as a  function of time.

I.I      Absorption curve of chlorine
Since this  problem has already been dealt with elsewhere,we
shall simply point out in Fig. 1 the various parts of the
curve allowing different principles of application.  It
should be remembered  that this curve, which gives the concen-
tration of  residual chlorine as a function of the amount of
chlorine introduced,  is plotted for a constant contact time.
This contact time is  generally made equal to the time of
transit through the treatment installation.

Point A corresponds to the  appearance of the residual
chlorine in the water.  The residual chlorine is then in the
form of chloramine, and treatment at this dose will be
characterized  by:

-        persistence  of ammonia,
         no improvement in  clarification  (decantation,
         filtration)  if the treatment is applied at the
         head  of the  treatment line,

-------
                           -  91  -

          slight intensification of unpleasant taste,
          slow action on microorganisms,
          no  formation of haloform compounds.
  Residual chlorine
  4 'mg/1
                     Determination of critical point
                                              Free «hl.
01    I    3   i   5   678   9
 Fig.  1   Chlorine absorption  curve
Point C represents the critical point;  it  corresponds to the
appearance of  free chlorine, and a prechlorination treatment
at this dose will have the following effects:

-        elimination of the ammonia,
         improvement of unpleasant taste,
-        considerable improvement of clarification,

-------
                          - 92 -    •.

-        effective and >ra_pid -action ;on,microorganisms •
         (phytoplankton and zooplankton),
-        formation of haloforms.

Treatment in zone B will lead to the formation of chloraraine-
type compounds, with absence of free chlorine and the appear-
ance of haloforms, while treatment in zone D will be charac-
terized by the presence of a considerable amount of free
chlorine.

1.2      Behaviour test
The behaviour test consists of introducing a certain amount
of chlorine into, the water, and following the residual con-
centration of chlorine as a function of time.  One then plots
against time either the concentration of residual chlorine
or the amount of absorbed chlorine.  The resulting curve
illustrates the kinetics of the reaction of chlorine with the
compounds present in the raw water.  It represents the
variation of the value of Cl_, shown in Fig. 1, as a function
                            3.
of time.
        24      48     72                     144

Fig. 2  Behaviour test: raw Seine water

-------
                         _ no _ •  .' -


By way of example', Fig." •"•a "shdws-'tlie^behavftour' 'test -'carried
out on a raw water.  It was ascertained  that  the  amount of
chlorine absorbed after 2 h of contact  (time  of the  passage
through the installation) is  3.5 mg/1.   After a contact time
of 24 h the amount of chlorine absorbed  increases to 5.3 mg/1,
and''after 144 h to 7.0 mg/1.  A raw water  directed  into a net-
work therefore consumes a large quantity of chlorine.

1.3      Consequences
It is therefore necessary to  find  a solution  that will allow
the production of a  water that is  as  stable as possible,
whose behaviour test gives  the flattest  possible  curve,
thereby indicating slight absorption  of  chlorine.

The  first  technique  used was  to  situate  the  first part of
the  curve,  where  the chlorine consumption  is  both fastest
-and  greatest,  as  early'as possible in the  treatment line.
This  is the so-called  "prechlorination"  technique,  in which
the  chlorine  is introduced  at entry into the  treatment
installation.  Under these  conditions the  chlorine  reacts
more  or less  rapidly with all the  substances  dissolved or
suspended  in  the  raw water  and with the  reagents  used during
the  treatment.
Prechlorination also has  the  advantage of  a  considerable
improvement in the  processes' of  coagulation,  decantation,and
even filtration.
Incidentally,  we  do  not intend  to  consider here  the technique
of  prechlorination with a contact  time of  over  24 h, which
can  be used when  the raw water  is  to  be  stored.
In  the above  case,  it  need  only  be indicated  that this tech-
nique can  result  both  in certain advantages   (reduction in
the  treatment concentration of  the coagulant) and in serious
disadvantages,  favouring the  conditions  for  the-formation of
chlorine compounds  (e.g. haloforms).

-------
                        -  94 -
II.      Factors influencing the absorption curve of chlorine
These factors can be due to the water itself or to the prac-
tical conditions of application of the chlorine ,

II.1     Composition of the water
The reaction, of chlorine with the inorganic substances
present in the water has been studied elsewhere.  It is
necessary to consider the possible reactions with the organic
constituents of the water.

II,1.1   Nitrogen compounds and the TOG
It is found that for one part of ammoniacal nitrogen it ^is
necessary to add 7.6 parts of chlorine in order'to obtain
chlorination at the critical point.  However, these^ propor-
tions are not constant.  Thus, while they apply to waters
containing ammonia and only a small amount of organic matter,
the ratio can rise to 15 for organically polluted water.
Pig. 3 gives two exampj.es for Seine waters containing
0.6 mg/1 of NH3, one of these coming from upstream of Paris
(permanganate oxidizability measured in an acid medium
4.3 mg/1), the other from downstream of Paris (permanganate
oxidizability 8.1 mg/1).
 It would obviously be  ideal  to  find a  formula allowing a
 calculation of the critical  point as a function of the con-
 centrations of various elements X, Y,  and  Z present  in the
 water of the  form:

 Treatment concentration for  the critical point
         = xf [X] + yf [Y] + zf  [Z]+ ...

 Various examples demonstrate the difficulty of finding such
 a formula.

-------
6
                            -  95  -
Residual C12                           - Seine upgtreag (*| Seine qownmtctfm
 >g/l           H-NHj measured, mg/1        -   0>s          0 5
              Organic matter KMnO, (H*> mg 02/1    4,3          g i
              Critical point value Clj rag/1      4,5      '    7_2
                  Critical point concn.
                      N-NH,           0.2         ,. ,
5 T
                 - Temperature: 15*c
                 - contact time 2 h
2-
1-
                       S    6    7   8    9    10
 Fig.  3^  Chlorine absorption curve
 a)        Example of glutamic acid
 Glutamic acid is an amino acid with the following formula:
 COOH-CHNH2-(CH2)2-COOH/
 containing  5 atoms of  carbon, which gives  a TOC value of 60 g
 per mole.

 Its molecular weight is  147.  In  addition,  one mole  contains
 14 g of nitrogen, i.e.  for 1 g of nitrogen there'are 4.26 g
 of total organic carbon, this corresponding to 10.5  g of
 glutamic acid.

 Incidentally, glutamic acid does  not correspond to  the
 amount of ammonia in water, at the very most a very  slow
 hydrolysis  being observed.  Fig.  4 shows the amount  of

-------
                          -96  -'•
50
25-
Co « 10 rog/1 of glutamic acid
   or about O.95 mg/1 of nitrogen
   or 1.22 mg/l of nitrogen expressed as
 Fig.  4  Hydrolysis of glumatic acid as a function of  time
nitrogen responding to an ammonia nitrogen determination as
a function  of  time.

The formula of glutamic acid leads one to expect a certain
reactivity  of  this substance with chlorine.  Fig. 5  shows
several curves for the absorption of chlorine by a solution
containing  glutamic acid.
The dilution water was first saturated with chlorine, then
dechlorinated  by means of ultraviolet radiation.  The absence
of chlorine absorption by the dilution water was then checked.
Solutions with various concentrations of glutamic acid were
used.  Fig. 5  shows the results of two experiments.  It can
be seen that the ratio between the value at the critical
point and the  amount of nitrogen introduced is 10.7  (as
opposed to 7.6 for ammoniacal nitrogen).

In addition, the general form of the curve is flatter than
that for a substance containing ammoniacal nitrogen.	

-------
10-
   Residual chlorine
                                    Solution of glutanic acid
                    Combined nitrogen mg/1
                    Organic carbon Hsg/1
                    ..Critical point value mg/1
                            (i)
                            0.5
                            S.13
                            5.3
 (2)
 1
 4.26
10.7
       - Temperature 2Q°C
       - contact time 2 h
Fig.  5   Chlorine absorption curve
b)
Example of  amyl alcohol
This  compound, CH~-(CH^)o-CEUOH, was chosen because it  too
contains 5 atoms of carbon per molecule.

The chlorine absorption curve shows negligible absorption of
chlorine.   One can find many examples of  substances having
various TOC and not giving rise to any absorption of chlorine,

c)        Example of pyridine
This  compound, CgH5N,has a high TOC and  contains one nitrogen,
It too does not lead  to any absorption of  chlorine  (see
Fig.  6).
d)        In conclusion,  it must be accepted that, while  the
ratio of chlorine introduced at the critical point to the
nitrogen increases with  increasing organic contamination of
the water, it is not  at  present possible  to link this factor
with the over-all composition of the organic contamination,

-------
                          -  98
 whatever method is used: TOG, permanganate oxidizability,
 UV absorption, etc.
    The ocganic carbon content
    of each compound in
       I
Fig. 6   Chlorine  absorption curve
II.1.2   Phenols
Pig. 7 shows the results  of  a  study into the elimination of
phenol products by chlorination of a water that also had a
high level of industrial  pollution,  the untreated mixture
containing various dimethylphenols and o-cresol. Chlorination
of these compounds results in  the appearance of a critical
point.  Below the critical point the formation of chloro-
phenols causes an intensification of unpleasant flavour,
while above the critical  point addition compounds saturated
with chlorine no longer correspond to the amount of phenol,
and the unpleasant flavour is  almost non-existent.

-------
                         - 99 -
    Phenol mg/l
                                             Residual chlorine
             10
Fig. 7  Elimination of  phenolic compounds by chlorination
        of  an  industrial water
Thus,phenol too participates in the absorption of chlorine in
water, and chlorination at the critical point allows the
organoleptic  properties of water to be improved.
II.1.3
Conclusions
It would  seem at  present that it is not possible to determine
the value of  the  critical point of a water from contamination
analyses  habitually carried out on water.  At the most, one
can assume  that,the ratio chlorine introduced at the critical
point/ammoniacal  nitrogen increases as a function of organic
pollution,  even though it is not possible to pinpoint this
.value within  the  range between 7.6 and 15.

-------
                           - 1OO -

 N                           '
 II.2     Variation  of the chlorine absorption curve
 Several factors may cause the dasorption curve of chlorine in
 water to vary.  Some of these may increase the chlorine
 demand of a water,  while others may mask it.

 II.2.1   Increase of the absorption of chlorine by water
 This concerns all reagents that may cause a supplementary
 absorption of chlorine,   h typical case is the use of  silica
 activated with ammonium sulphate.  Fig. 8 shows its  effect:
   Roidual chlorine
      aS/1
    (1) Jttt + AS

    {2} RH + AS + 1 mg/1 Si02 (0.13 nj/1 H)

    (3) BH + JW + 3 mg/1 810, (0,40 mg/1 N)
                                            Chlorine introdueed.nj/1
                                               =	
 0
1   I    3    i   5    6    7   8    9    10
Fig. 8  Effect of  the use of silica activated with
        ammonium sulphate
for  treatment concentrations of activated silica (SiO2) of
between 1  and 3 mg/1/ i.e. for amounts of nitrogen (expressed
as N)  introduced into the water equal respectively to 0.13
ana  0.40 mg/1,  one observes an increase  in the value of the
critical point, which rises from, 3.2 to  4.2 and 6.2 mg/1 of
chlorine.   At the same time, the form of the absorption
curve  changes,  tending towards the typical form obtained with
a pure solution, of ammoniacal nitrogen.

-------
                        - 1O1,—   -

11,2.2   Effect of powdered activated carbon
In the' face of the degradation of the organoleptic properties
of raw water, activated carbon has been used more and more
often.  The powdered fo'rm is'now used most, f requently, at
least at the start, when -the 'use of the activated carbon may
be periodic.  The carbon is introduced during coagulation-
flocculation, in order to profit from the time of contact
required for the flocculation. •

The use of powdered activated,carbon in diffuse flocculation
units, situated upstream of static or plate decantation
units, does not involve any appreciable change in the methods
of application of prechlorination,

In contrast, in the case of sludge-bed decantation units
(Accelator or Pulsator types), some, changes in the observed
phenomena do occur.

Thus,'the use of a treatment  concentration of 15 mg/1 of
powdered activated carbon allows a concentration effect to be
obtained in the sludge bed, where the concentration of acti-
vated carbon can reach 1 g/1.  This has a favourable effect
on adsorption, and it is important to determine more precisely
the effect on prechlori-nation.

Pig.  9 shows chlorine absorption curves obtained in an instal-
lation with and without activated carbon.

A. very flat curve is found in the case of the treatment using
powdered activated carbon,  which is advantageous for oper-
ation: a sudden variation of  the critical point will not
disturb the residual concentration of chlorine in the treated
water.  It is important, on the other hand,  to determine its
effect on the treatment of the'water itself.  To this end,
the installation,  which included a Pulsator decantation unit,

-------
                          - 102 '•*
 R nidual chlorine.
B-

5-

4-
Baw Seine water
Temperature 20°O


(1) RM t AS

(2) RW + ftS + ftC (15 mg/1)
                         5    f   7   8    9
                                         Chlorine introduced, mg/1
Fig. 9  Absorption of chlorine with  or  without
        powdered activated carbon
was operated with prechlorination  treatments at points A
 (2.5 mg/1,  i.e.  below the critical point), B (4.25 mg/1,  at
the critical point itself), and  C  (6 mg/1, i.e. above the
critical  point).

The water,  industrially filtered through sand,  was sampled
after each  of these treatments,  and the chlorine absorption
carve was plotted for each sample.
The results  (Fig.  10), lead to the following observations:

-         in  water  C there is no  secondary consumption of
          chlorine,
-         in  water  B the absorption curve develops normally
          without any significant secondary consumption,

-------
                            -  103-i.T-  -

           in water A,  a  secondary absorption  of chlorine and
           the appearance of  a critical point  at a treatment
           concentration  of 1.8 mg/1 are observed.  If one
           adds together  the  treatment concentration and the
           concentration  used previously during' decantation
           with powdered  activated carbon  (2.5 mg/1), one
           obtains a total of 4.3 mg/1, while  the treatment
           concentration  without activated carbon is.4.1 mg/1.
    Residual chlorine,     •'     '        Tests carried out on water prechlorinated
                                 with a treatment concentration ;
                              (C)   above

                              (B)   equal to
the critical point
                                     Chlorine introduced, mg/1
 0     11345    6
 Fig.  10  Chlorine  absorption curve for a water
          treated with powdered activated carbon
The excess  chlorine absorption  is  thus only 0,2 mg/1 for  the
installation  under consideration,  the introduction of  acti-
vated carbon  being carried out  30  sec after the introduction
of the chlorine,  for the same quality of raw water.

The use of  powdered activated carbon under these conditions
has hardly  any effect on the prechlorination treatment,apart
from a very slight excess absorption.

-------
                          _  104' -    '


Incidentally,'ir'eceht studies'havfe Shown'that it was futile,
from the bacteriological point of-view, to provide a raw
water-chlorine contact time of the order of 15 min before
the introduction of the powdered carbon.-

Prechlorination can therefore be carried out without any
drawbacks, in a decantation unit using powdered active carbon,
the carbon not impeding the oxidizing 'action of chlorine on
products that contain nitrogen in the form of ammonia, but
allowing the adsorption of certain precursors of the form-
ation of haloforms.

III.     Factors influencing the behaviour of the test
In certain cases the chlorine demand of water varies without
the chlorine absorption curve showing a proper critical point.
The action of ozone interferes'with the chlorine, and it has
proved possible to demonstrate a relationship between the
humic acid' index and the chlorine demand of a water.

Numerous tests have been carried out on this interference,
which can be summarized in the following experiment.
Raw river water is subjected to the following treatment:
-        prechlorination with a dose higher than the
         critical point  (or break-point),
—        coagulation-flocculation-decantation,
-        adsorption treatment with powdered activated
         carbon added to the decantation unit,
-        filtration.         •.  .

-------
                           - 105.
This produces a treated water ,of. excellent quality,  whose
characteristics are as follows:
          pH            ......         :   7.4
          Colour (mg/1 of Pt  Co)            :   5
          Suspended matter  (mg/1)           :   0.3
          Turbidity (JU)                    :   0.1
          KMnO4 oxidizability.
          (acid medium, mg O-/!)            :   1.25
          CAT  (°F)     •   •    •             _ 16
          HT (°F)        ...                 : 25
          Anionic detergents  (mg/1)         :   0.10
-         Phenols                           : not detected
This  treated water  (water T)  was subjected  to a chlorine
absorption test as  a  function of time, the  results of which
are given in Fig. 11.
 0,6
 0,7-
 0>
 0,5-
 0,4-
 0
 0,2
 an
    Residual chlorine,
      mg/1
water T •+• 0.8 mg/1 of chlorine
                               - ,   *         Time* min
       -I	1	1	\	r~
  0    10   20   30  40   50   60. -70  80   90   100
Fig. 11  Chlorine absorption curve  for water T
         as  a function of time

-------
                           - 1O6 -
It can  be  seen that the water absorbs  practically no more
chlorine,  which would seem obvious a priori,  since the water
was previously treated with a treatment concentration higher
than the critical point value.  This applies  to the two treat-
ment concentrations tried: 0.3 mg/1 and 0.8 mg/1, and the
chlorine absorbed in 100 min was respectively 0.03 and
0.06 mg/1,  which is of the order of the experimental error.

Fig, 12 shows the same water T treated again  with 0.3 mg/1
of chlorine to check the absorption of chlorine in this
samplej the absorption is virtually non-existent.
  Residual    .  Introduction of
  oxidiilhg agent  oWorinE  0.3 mg/1
          '
Introduction of ozone
       .15 mg/1
       .52 mf/1
Introduction of chlorine
0.75 mg/1
          0   10  "20  300  10   20   30   40 ' 50iO  10  20
Fig.  12  Effect of ozonization  on  the absorption
          of chlorine
Ozonization  of  the water was carried out  after  30 min of
contact.  The treatment concentration was 0.8 mg/1 (or 1.15 mg
if this dose is expressed as a chlorine'equivalent,  as on the
graph) which ensures an ozone residue.of  0.35 mg/1 (or 0.52
expressed as the chlorine equivalent)-.  The  total contact
time in two  contact columns was 8 min: 4  min plus 4  min.

-------
                        - 107 -


The water was then left to stand and the total residual
oxidizing agent and the total residual chlorine were measured.
The difference between the resulting curves represents the
evolution of the residual ozone, expressed as chlorine.  A
reduction in the amount of residual ozone is found, which is
normal, but there is also a reduction in the residual
chlorine: consumption of chlorine does, therefore, take
place,in this case in an amount of 0.2 mg/1 after 50 min of
contact.  To confirm this finding, a new chlorine absorption
test was carried out: 0.75 mg/1 of chlorine was introduced into
the water,and the residual chlorine was checked as a function
of time: a sudden fall in the residual chlorine was found:
0.25 mg/1 was absorbed in 10 min and 0.30 mg/1 in 30 min.

In all, the ozone treatment thus caused a secondary con-
sumption of chlorine, amounting to 0.5 mg/1 in the case in
question.

In an attempt to define more accurately this secondary absorp-
tion of chlorine, trials were carried out in another install-
ation treating a Loire water.  Fig.13 shows the variation of
the chl'orine demand of this water as a function of time for
up to 24 h of contact.  The demand varies according to the
stage of the treatment, and we have compared it to the amount
of humic acid present.  Fig. 14 shows the relationship
obtained between these two factors.

It can be seen that the reduction in the amount of humic
substances allows the chlorine demand of a water to be con-
siderably reduced.  This elimination will at the same time
reduce the quantity of certain haloform precursors during
chlorination.

-------
                                       -  108  -
      pg/1 of C12
           Coagulation, floeculntion, decantation, filtritibn through aind- '


           Congelation, flocculation, decantation, filtration through und * oxofliutioa


           Coagulation, flocculation, decantation, filtration through «and * oxoniutie*
           * filtration through carbon
 Fig.  13   Chlorine demand  of  waters
         Chlorln* <3«a«nd
            •9/1




.
1 1
1,

!
, '-I
:
i
.


•

|
i -

i |

i
i I
t





	 	 	 ,-,-.

i! '

! I | :•
( ^uaie' acW(| mg/t
1  raw water
2  
-------
                         - 1O9 -

IV.      Conclusion                         .,-  '    ....
Taken together, these observations allow a determination of
the procedure to follow in the application of a chlorination
technique:

-        Avoid chlorination in conjunction with prolonged
         storage of raw water.
-        As far as possible, eliminate organic and humic
         material by regulating the clarification treatment
         more precisely.  In particular, it will be necessary
         to adjust suitably the treatment concentration of
         the coagulant, for the sake of a better elimination
         of colloids and organic matter, and to avoid insuff-
         icient treatment by seeking an acceptable turbidity
         while allowing organic matter capable of being
         .eliminated at this stage to remain.
-        It is possible to use a small dose of powdered
         activated carbon at the coagulation-flocculation-
         decantation stage.  The sludge-circulation or
         sludge-bed decantation units favour this effect.
         In the case of prechlorination, treat at the
         critical point, but avoid treatment too far
         above it.
-        Again in the case of prechlorination, adopt
         sufficiently high rates of decantation and
         filtration to avoid conditions favouring the
         formation of haloforms.

In all these cases a complete and well applied treatment, as
regards both clarification and adsorption on granulated
activated carbon, should allow the potential of chlorination
to be utilized as far as possible, while avoiding the adverse
effects that chlorination can provoke if it is incorrectly
carried out.

-------
                           110
CHARACTERIZATION OF ORGANIC WATER CONSTITUENTS BY THE
KINETICS OF CHLORINE CONSUMPTION
H. Bernhardt and O, Hoyer


1.     Introduction
With increasing eutrophication of the Wahnbach dam in recent
years the chlorine demand for the disinfection of treated
drinking water increased as well.  Only with the aid of
repeated post~chlorinat5on in the distribution network
could it be ensured that the water in the towns and communities
supplied still contained some chlorine.  In spite of this increase
in post-chlorination, in recent years sudden bacterial growth has
been occurring more and more often in some parts of the pipelines
a,nd overhead tanks.  This growth pointed to a reduced safety
of the drinking water supply and was the reason why we started
a detailed study into the extent and the course of the chlorine
consumption in water.  In the "course of this work we discovered
that the dam water consumes different amounts of chlorine at
different times of the year.  Fig. 1 shows that the chlorine
consumption of the dam-water to be treated is essentially lower
during winter than during the summer, when intensive growth of
algae takes place and algal organic compounds are released into the
water.  These changes in the quality of the dam water, however,
cannot be-detected by determining the content of dissolved
organic compounds (DOC), since the concentration change of the
DOC is relatively small.

Detailed investigations, carried out because it had been
shown that the flocculation process in the treatment of
the dam water with aluminium salts is affected by the compo-
sition of the biogenic organic substances in the water, have
demonstrated the influence of algal substances on water
treatment (1).

-------
                          -111
   (tng/l)
•
.
1.5-
_o m
a

8
] 1,0-

1
6

-
^
as-


*
.

™
0 -
Dan xat*r *ro»*
>X A 10.1.74
:^V 'B 8.8.73
Vi^ c 24-5>73
V^N,


\ X« ^****«j
\ *^>*^ ^*-~-+^4
• "*""•+«. ~~
\» •'•••^
\ *--*—*._
*» *''*"D
\
X
\
\
\
\
\
\
\
*c

6 60 120 180 240 300 360 420 430
Reaction • time fmin)
                                   Fig.  1
                                   Chlorine consumption curves
                                   for dam water at various
                                   times of the year
Determination of the chlorine consumption as an analytical
procedure for characterizing water with respect to its
organic substance content has been performed for years, but
has lost ground in recent years.  A report on the various
procedures used for the determination of the chlorine con-
sumption of water will be found in an  earlier paper  (2).
Since the latter work also contained a detailed bibliography,
this will be omitted here.

2.     Investigations of chlorination kinetics
If a certain amount of chlorine is added to a water sample
and the fall of the chlorine concentration is followed con-
tinually as a function of time, statements can be made about
the kinetics of the chlorine consumption.  For this purpose
it is useful to plot the logarithm of the chlorine concen-
tration measured in the sample against time.  Fig. 2 shows
the course of the chlorine consumption for two different

-------
                          - 112 -
           200   400    600   800   1000
 Fig.  2   Chlorine  consumption in water  samples  from the
         Wahnbach  auxiliary dam (inlet)  and from the
         filtrate  of the  P-elimination  plant (outlet)
         (27  July  1978)
waters in such semi-logarithmic representation.  One of the
samples was water from the Wahnbach auxiliary dam, considered
as strongly eutrophic, and the other the filtrate from a
phosphorus-elimination plant by the Wahnbach dam, in which
the content of organic substances is diminished by 50 - 70%
by the treatment step, depending on which parameters are
used to evaluate the organic substances.  Prom these curves,
which a re reproduced schematically in Pig. 3, it can be seen
that the chlorine consumption can be resolved into s
a)     a spontaneous consumption that takes place extremely
rapidly and cannot be followed as a function of time with
our measuring procedures,

b)     a rapid chlorine consumption within the first 3 h  (in
the shaded region) and

-------
                          <- 113-



c)     a slowly progressing  chlorine consumption, which can

be observed over the subsequent longer period of time.
                                               !ieasured values

                                               Calculated value:
              200     400     600    800     1000  Reaction tit*
Fig. 3  Diagram for  the  calculation of magnitudes from
        the course of  the  Cl2-concentration against
        reaction  time. The .spontaneous consumption is
        not included in  the C12-concentration at time t
O
The semi-logarithmic  representation shows that the slow

consumption of chlorine takes place linearly with time, so

that this slow consumption phase can be described by a first-
                                          2
order rate equation whose  rate constant K  is calculated

from:

                    K  =  In C/CQ .  t"1  (min'1)
To obtain the  rate  constant K  of the fast reaction, the

fraction of the  chlorine concentration corresponding to the

slow consumption rate  is extrapolated to t = 0.  By sub-

tracting this  fraction of the concentration from the total

-------
                           - 114 -
chlorine concentration  present  at  the  time,  the  fraction  of ,
chlorine concentration  corresponding to  the  fast reaction is
obtained.   Plotted  semi-logarithmically  against  time,  this
fraction of the concentration also gives a straight  line, and
thus also corresponds to  a  first-order reaction.   It is
remarkable  that the complex chlorine-consumption reaction
can be  represented  empirically  by  two  superimposed first-
order reactions with rate constants differing  in nearly all
'cases by two powers of  ten.  As Fig. 2 shows,  for the water
sample  from the auxiliary dam  (inlet):
              K1 =0.4  • Kf2 (min"1)     and
              K2 - 6,45- 10"4 (min"1),
while  in the case of  the  chlorine  consumption  by  the  filtrate
from the phosphorus-elimination  plant no value for  K-'- was
found  and K2 was only" 2.12'10~4  (min"1).

The higher  the rate constant measured,  the  faster does the
chlorine consumption  take place  in the  respective water
sample.  The rate of  this chlorine consumption is determined
solely by the nature  and  the amount  of  the  organic  substances
present in  the water.   It was  found  that ammonium ions are  not
                                 1       2
included by the rate  constants K  and K .   With the aid of
continual measurements of the  chlorine  consumption  and the
determination of the  rate constants, it is  also possible to
characterize a water  with respect  to its content  of organic
material.   Continual  measurements  of the course of  the
chlorine consumption  therefore constitute a further sum
parameter for the evaluation of  water,  with particular
reference to the chlorine-consuming  organic substances.

-------
                          -115-


3.     Performance of the chlorine-consumption measurements
The measurement of the chlorine consumption as a function of
time is carried out on 5-litre samples in brown glass bottles.
This volume size eliminates the possibility of a wall-effect
(cf. Maier and Mackle) (3), which can simulate a high and
unrealistic chlorine consumption.  We have already reported
on this point  (2).

The fall in the chlorine concentration against time is
measured continuously with an automatic analyzer.  For this
we set up the Autoanalyzer 2  (Technicon) in accordance with the
German unit process G4 (DEV G4) , in. such a way that during a
measurement over 24 h the water volume of the sample decreases by
only 20% at the most.  The measurement region was between
0.05 and 10 mg/1 of chlorine.  To be able to measure the
sample with the Autoanalyzer the sample must be free, from
particular substances, and it is therefore necessary to filter
the test sample through a membrane filter with pore diameters
of 0.45 jnn.  The method for the determination of active and
free chlorine for the Autoanalyzer input according to the
DEV G 4 is described elsewhere (4).

4.     Influence of the chlorine-carbon ratio on the chlorine
       consumption kinetics
The chlorine reaction here under discussion is a complex
reaction of many organic compounds, not known individually,
with only one reaction partner, chlorine.  Since the differ-
ent materials react with the chlorine at different rates, a
sufficient amount of chlorine is necessary, so that the
fast reactions are not preferred to the simultaneously
occurring slow reactions.  If this were allowed to happen,
the fast reactions would use up the .available chlorine too
quickly, so that not enough chlorine would be left for the
slow reactions, and the course of the over-all consumption
would be falsified.  Therefore, the chlorine-carbon ratio

-------
                          -r 116 - :   •


established at the outset of the 'kinetic measurements is of
decisive importance.

Our detailed investigations were concerned with the influence
of the chlorine-carbon ratio on the ira'te constants.  For
these experiments we used as typical water constituents high-
molecular weight organic acids  (humic  acids)  isolated from the water
of the Wahnbach dam in April 1978.  We obtained these humic
acids by alamine extraction and molecular weight separation
with an Amicon UM-2 membrane, exclusion limit MW 1000 (5,6).
The procedure was to add a suitable amount of DOC to a 5-
litre sample with a specified chlorine content.  Blank con-
sumption of chlorine could thus be eliminated.
(mgfl m _.'
0,8
I *&
s
*i
*l
5 0#
c
0-
r /
/
Y C?- 0.24 -DOC (ri-0.95)
/ (without •)
A
/ W

y»
. nnp
) i 2 3 4 i^g/D
eye:
• 1
(mm) ^ ! |
10'2
u
I to-
Sl
a op.
0
1C
(.)
_______.. — •
r" K®- 0.7 * 0.05 • DOC(r2-0.68)
(without • 1
•~ DOC
) 1 2 3 4 (m3/D
Fig.  4   Initial  concentration cQand rate constant KO for
         the  fast partial reaction(T) of the chlorine con-
         sumption in  dependence of the DOC with various
         C12/C ratios

-------
                           -117-
 The  results  of  these  experiments  have  been,compiled  in
 Figs.  4  and  5.   Pig.  4  shows  that the  rate  constants of  the
 first  fast chlorine consumption  (K ) for  chlorine-carbon
 ratios (weight  concentration  ratios) of 2 or  3  in  the region
 of 1 - 4 mg  DOC/1'are approximately constant.   With  a
 chlorine-carbon ratio of more than 2,  on  the  basis of the
 chlorine excess then  present,, the initial chlorine concen-
 trations C   obtained  by extrapolation  (see  Fig.  3) are pro-
 portional to the concentrations of dissolved  organic carbon
 (DOC)  present in the  water  (see, the upper part  of  Fig. 4) .
 This shows that above a chlorine-carbon ratio of 2 the
 chlorine concentration  provided is sufficient for  the fast
 chlorine-consumption  reactions.  .             ,

 For  the  slow chlorine-consumption reaction  the•situation is
 not  so favourable.  Fig. ,5  shows  that  rate  constants are not
 independent  of  the DOC  until  the  chlorine-carbon ratio
•reaches  or exceeds 3f and then retiain  constant.  At a chlorine-
 carbon ratio of 2 there is  still  a slight variation  of the
 rate constants  with the DOC.   A chlorine-carbon ratio 1  means
 in all cases an insufficiency of  chlorine.  This can also be
 derived  from the dependence of the initial  chlorine  concen-
          2
 trations C   obtained  by extrapolation  (cf.  Fig.  3).   Not
 until  a  chlorine-carbon ratio of  more  than  2  has been reached
 is  the  chlorine  excess  provided  sufficient  for  an  optimal
 chlorine-consumption  reactio:
 are proportional to the DOC.
                                       2
chlorine-consumption reaction and the C  values calculated

-------
                           - 118 -
 (rng/l)
   6-
  •S2-
  (rain1!
  15'
                    eye=2
                   ct/c=i
                1    I
     01234 (mg/l) DOC
CI/C=1
                  Cf/C=3
                           DOC
        1234 (mg/I)
 Fig.  5  Initial concentration CCyand rate  constant
         the slow partial reaction  (?) of  the chlorine con
         sumption in dependence of the DOC with various
         C12/C ratios
An attempt was also made,  by forming differences, to calcu-
late a rate constant  independent of the actual chlorine con-
centration in the  samples  and thus independent of the chlorine-
carbon ratio.  For this  purpose the -chlorine concentration
after 1000 min of  reaction time (C^QQO min^  was sub"trac"te
-------
                          - 119 -


increase in proportion to the DOC content and independently
of the chlorine-carbon ratio.  Unfortunately, however, the
rate constants K calculated in this way  (the lower part of
Fig. 6) for the DOC-concentration range of 1 - 4 mg/1 are not
as independent as was hoped.  The scatter of the individual
values for the different chlorine-carbon ratios is so unsys-
tematic that no curve must be drawn through these points. The
same unsystematic variation of the results was observed in
the evaluation of the kinetic chlorine consumption experi-
ments on different waters.  These relative rate constants
were therefore not introduced as a means of characterizing
waters.
(mg /I
2.0^
1.0-
0.5-
A

(mirr1
io-34.
„ 3-
c
8 2-
D
1-

Fig
!cr /
/*
, CI./C •
/ • t
*^ .2
X* 3
-
• 1234 toia /I)
'K® •
» * •
. * -
•
•
3 1 2 3 4 (mg/l)
. 6 Initial concentrat.
                           DOC
                               CJ  and  rate  constant  K  ^
         for  the  chlorine  consumption  obtained  from  the
         difference  in  the chlorine  concentration  after
         1OOO min of consumption  in  dependence  on  the  DOC
         with various C12/C ratios

-------
                           - 120 -  •

5.     Formation of organochlorine compounds during a 20 h"
       chlorine action period
Both the high-molecular and low-molecular weight organochlorine
compounds are formed in the reaction of chlorine with a
mixture of organic substances in water.  In this respect it
is interesting to ask whether there is a connection between
the chlorine consumption and the amount of organochlorine
compounds formed.  Therefore, at the end of a chlorine
reaction'time of 20 h the total content of organic chlorine
compounds formed (TOC1)  and the content of haloforms were
calculated and related to the concentration of organic carbon
compounds (DOC), with an allowance for the chlorine-carbon
ratios in each case.  The results obtained for our model
substance (humic acid as auxiliary dam water) are collected
in Fig. 7, according to which both the formation of halo-
forms and of the total organic chlorine (TOC1) takes place
in proportion to the concentration of organic compounds in
the water samples, irrespective of the selected chlorine-
carbon ratio.  Thus, above a certain minimum chlorine amount
it does not matter for the formation of TOC1 and haloforms
how high the concentration of chlorine is in relation to the
DOC concentration present, provided that a sufficiently long
reaction time is allowed.  The experiments have shown that a
period of 20 h is sufficient.  This finding is decisive for
the practice of water treatment in respect to the chlorin-
ation and the transport times of the water to the consumer.
It shows that, independently of whether the water has been
subjected to preliminary chlorination, high chlorination, or
break—point chlorination, increasing concentrations of organo-
chlorine compounds in ihe water must be reckoned with when-
ever increasing concentrations of dissolved organic compounds
are present in the water to be'treated if the compounds are
capable of reacting with chlorine (precursors).  Further
trials (see Fig. 12) have shown that this proportionality is
also observed for natural water with an unknown mixture of
organic compounds.

-------
                          - 121 .-
 200-
 150i
 100-
3 50
        TOCI« 41,6/27.7-DOC (r2=0.85|
        HaIoforms=11+6.4  -DOC (r2=0,59)
Fig. 7
TOC1 and haloform formation
in dependence on the DOC
for various C12/C ratios
(reaction time 2O h)
        1234  (mg/l)
             DOC
6.     Application of  the method'
The practicability of  this  method of continual chlorine-
consumption measurements was  checked in the period of June
to August 1978 on numerous  water samples from the eutrophic
Wahnbach auxiliary dam, on  the filtrate from the phosphorus-
elimination plant, and on water samples from the mesotrophic
Wahnbach dam itself.   In addition, during the same period
corresponding trials were performed on water samples from
the Rhine at Bonn to determine whether this process is also
applicable to surface  waters  polluted not only by domestic
sewage but also by industrial effluents.

-------
                          -  122  -
•10-
    10-
     5-
 (nr'J
(mg/l)
ICC
                           1C®
Extinction
 (280 nm)


DOC
Fig. 8
Time variation of the usual
quantities in comparison with
the chlorine consumption rate
constants K CD and K© in
water  samples  from'the
Wahnbach  auxiliary  dam
Pig. 8 shows the rate constants  from  the  chlorine  consumption
measurements on water samples  from the Wahnbach  auxiliary dam
for the investigation period.  They are compared with the UV
extinctions at 280 nm and with the DOC contents  in these
water samples.  A really good  correspondence  of  the course
                       1       2
of the rate constants K  and K  and the sum parameters of UV
extinction and the DOC can be  seen.   In both  cases the rate
constants obtained were plotted  on the diagram as they
resulted from the concentration  changes in free  active and
total active chlorine.  With the exception of two  measured
points, these K-values hardly  differ  from one another.  It
should be noted in this context  that  in June  1978  there were
still analytical difficulties  in the  performance of continual
chlorine-consumption measurements, and for this  reason only
one value was available for June.

-------
                           - 123 -
            1      2
If all the K  and K  values obtained in this period for the
three waters are plotted against the UV extinction at 280 nm
(see Pig. 9), a clear dependence on the concentration of the
dissolved organic substances responsible for the.'UV extinc-
                                                          2
tion is observed, especially in the case of the constant K
of the slow reaction.  The values for the auxiliary dam water
to the right of the broken line in Pig. 9 approximately
continue the course of the measurements from the other
samples.  However, no straight regression line can be drawn
through these points, since at present there are still not
enough measurements for extinctions greater than 6 m

On the other hand, the connection between the rate constant
of the fast reaction K  and the content of organic substances
in the water, that cause the UV extinction at 280 nm, is not
so clear.  A dependence appears to exist for the auxiliary
dam water (to the right of the broken line) but not for the
water samples from the filtrate of the phosphorus-elimination
plant and the Wahnbach ^dam itself (left of the broken line) .
A possible explanation may be that during the treatment of
the auxiliary dam water, rich in algae and humic materials,
a selection of the organic substances takes place, in which
the high-molecular materials are preferentially removed from
the water, causing a proportional increase of the low-
molecular organic compounds in the filtrate of the elimin-
ation plant and therefore also in the Wahnbach dam (7).
Further studies will show to what extent there is a dependence
of the chlorine reaction rate on the molecular weight range
of the organic substances present in the water.

-------
                           - 124- -
(min-1)
•10-2

1.5 •




1.0 •


0.5 -


0 -
(min" )
•10~4
ifCt)
Kw T _ 15 -
! *
i
, i *
• s 1 1 .
••£• ! I 10 -
U k
i i
4 I
.! § -
* free active chlorine
1 total active chlorine
	 . 	 , 	 , 	 , 	 , n -


« jr/2J
l\ * <




A
, *
ii1
i i«
»J| »|t .
.%» i •

          2468
          Extinction (280 nm)
02468   lOfnr11)
      Extinction (280 nn!
 Fig.  9  Rate constants of the fast  (K (*))  and  slow (K (£))
         chlorine consumption reaction plotted  against the
         UV extinction of water samples  from  the  Wahnbach
         auxiliary dam, from the filtrate of  the  P-elimination
         plant, and from the Wahnbach dam (June-August 1978)
Pig. 10 gives the results  of  experiments on Rhine water
samples for the period June to  August 1978.  Changes in the
                              1             2
rate constants for the slow  (K  )  and fast (K )  chlorine-
consumption reactions are  plotted,  obtained from the concen-
tration changes of free  active  and  total active chlorine, as
well as the UV extinction  at  280  nm and the DOC content.  To
facilitate an interpretation  of the results,, the river flow
of the Rhine during this period has also been plotted.  Here
too a relatively good parallel  is evident between the indi-
vidual quantities, but most of  all  between the UV values and
the K1.

-------
                            -  1"25 -!
(min'1! 15 -
    ID-
     0
 (mitr1! 2
  • t
-------
                            - 126 -
(min-1)
.10-2
3 -


2 •
c
3
§
§

1 1 -

0 -
(min"1
if© -io"4
l\ 15-


I I -

•


•
1 1

i — i — i — i — i fl-

IC®
<

i ,
•




:


i

i
i






* free
B tota











active chlorine
. active chlorine

        4567  8(m-1)   4567   Sdir1)
           Extinction (280 ran!
                                   Extinction {280 nmS
Fig.  11   Rate constants of the fast K(2)and the slow K©
          chlorine consumption reaction plotted against the
          UV extinction of water samples from the Rhine at
          Bonn (June-August 1978)
7.     Connection between  the  rate  constants and the form-
       ation potential for organochlorine  compounds
In Fig. 7 the proportionality  between the  formation potential
of organochlorine compounds and  the content of  organic solved
compounds was clarified  in the light of  experiments with the
prepared humic acid from auxiliary  dam water.   Similar experi-
ments were performed on  water  samples from the  Wahnbach
auxiliary dam, the Wahnbach dam  itself,  and the filtrate of
the phosphorus-elimination plant.   The results  are reproduced
in Fig. 12. Here too, like in  Fig.7,  there is a really good
correlation between the  amount of TOC1 or  haloforms formed
after a reaction time of 20 h  and the content of organic

-------
                           - 127 -

substances causing UV extinction  in  the water samples (values
to the right of the broken  line  : Wahnbach  auxiliary dam).
Such a connection is also found for  water samples  from the
Rhine.  It is noticeable that in  the water  samples from the
Wahnbach dam,itself  (left of the  broken line)  the  essential
part of the TOC1 is due to  haloforms
  200-,
(jig/I)
   150-
  100-
   50 J
    P-
     02    4    6    8   10(m-n

              Extinction 280 run
Fig. 12
TOC1 and haloform formation
potential in dependence on
UV extinction in water samples
from the auxiliary dam, main
dam, and filtrate from the
P-elimination plant.
If the rate constant-:, K   for  the  slow reaction are plotted
against the corresponding concentrations  of organochlorine
compounds  (TOC1 and haloforms)  formed by  the chlorine-
consumption reaction, a clear dependence  is again observed.
The amount of TOC1 formed and of  haloforms  increases with
increasing reaction rate, but not without limit, as can be
seen from the exponential course  of  the curve for the

-------
                           V. .128 -
K^ - TOC1 system  in  Fig.  13.   On the other hand, this conn-
ection is not  so  clear  for the fast reaction, expressed by
the constant K  (see Pig.,14).
(mitr1)
10-<
16 -
14 -
12 •
a 10 -
e
41
I »-
1 6 -
4 -
2 -
^ 10'4
|{© 18 •,
| , 14-
/ 12-
* / 10 -
i / i'-
•X
*^ Jt w
X% H-
*
	 	 ..... . n -
K®
i (
/
/
l'/
A /^ g ^ ^free active chlorine
•/Jf total active chlorine
1 / *
     0  50  100  150 200(fjg/l)
0  50  100  ISO 200(jjg/l)
Fig.  13   Connection between the slow chlorine-consumption
          reaction (2[) (rate constant K© ) and  the  formation
          of  organic chlorine compounds  (TOC1)  and haloforms
          in  water samples from the Wahnbach auxiliary dam,
          the filtrate of the P-elimination plant, and the
          Wahnbach dam (June-August 1978)

-------
 (min-1)
  10-2
   3
                           '-.' 1 29 -
(miir1)
 10-2
  2 -
   1 -
  2 J
  1 -
                                       '•<•<;• .i<-t ,..-.. i-hlnrinr
                                       t'.(.,] ,„-. JV|. ,-hl,,rin(.
    0   50   100 150  200(ug/l)   0    50  100  150  200tig/l)
            •I'oc.-i                       .,.,,'
Fig.  14   Connection between the  fast  chlorine-consumption
          reaction  (T)  (rate constant  K ©)  and the formation
          of organic chlorine compounds  (TOC1)  and haloforms
          in water samples from the Wahnbach auxiliary dam,
          the filtrate from the P-elimination plant and the
          main Wahnbach dam (June-August  1978)
From these results  the  over-all conclusion can be drawn
that within certain limits when the extent of the formation of
organochlorine compounds  is greater/ the faster  is  the
chlorine consumption reaction in a water sample.  Detailed
investigations on this  entire complex are, however/  still
outstanding.

8.     Experiments  on chlorine consumption with  algal
       suspensions  and  algal culture filtrates
The organic substances  released by algae, insofar as they
have a saccharide structure, respond only inadequately to
the DOC determination used by us with UV oxidation  (8).
Depending on the algal  species, DOC values that  are essen-

-------
                           - 1 30 -

 tially  too  low are  obtained under  certain circumstances,  so
 that  the  chlorine-carbon  ratio  for the  continual chlorine
 consumption measurement cannot  be  sufficiently securely
 established,  for  which reason preliminary experiments to
 obtain  the  expected chlorine consumption are advised.  On
 account of  the associated uncertainty of the values obtained
 we  have as  yet made no statements  on the evaluation of the
 chlorine  consumption  rate, although differences dependent on
 the algal type can  be discerned.   A comparison of the chlorine-
 consumption kinetics  for  algal  suspensions and algal membrane
 filtrates  shows  differences that  may well be connected with
 the stability of  the  algal particles, although the course of
 the consumption is  essentially  similar.  Further investi-
 gations on  this are envisaged,  which should also take into
 account the age and the stage of the algal culture.

 Formation of  organochlorine compounds by chlorination of
 algal cultures and  algal  culture filtrates
 Pig.  15 shows the course  of the TOC1 formation plotted against
 the reaction  time of  the  chlorine  consumption, for suspensions
 of  some algal species*.   The particulate organic carbon (POC)
 content in  these  trials was approximately 60 mg/1; the
 Pseudanabaena suspension  contained  only 40 mg POC/1.  The
 TOCl  formation with this  alga was  very pronounced and,
 referred  to particulate organic carbon, nearly double that
 with  the  green algae  Carteria and  Pandorina which were also
 studied.  The siliceous alga Fragilaria, on the other hand,
 has the lowest TOCl-formation potential.  A corresponding
 series  of measurements with water  from the Wahnbach auxiliary
 dam of  6.7.1978 fits  in well with  these data.  At that time
*The strains used came from the collection in the Biological
 Laboratory of the WTV,  isolated by Dr.  Clasen:
           Pragilaria crotonensis  C 68/6
           Carteria radiosa        C 69/15
           Pandorina morum         C 72/2
           Pseudanabaena galeata   C 67/2

-------
                           -  131  -
the algal population had risen to 200,000 individuals/ml,
consisting chiefly of microalgae and flagellates.  It is
typical for all algal species that the bulk of the TOC1 —
nearly 90% — is formed in the first 5 h of the chlorine
reaction.
 400 i
(jig'0
 300 -
 200 J
 100 -
Fig. 15
TOC1 as a function of the
chlorine consumption time
in unfiltered algal cultures
and in auxiliary dam water
during an algal bloom
(2OO,OOO individuals/ml)
                             25 (h)
If, however, the haloform formation is plotted against the
reaction time of the chlorine consumption  (Fig. 16), it  is
noted that, with the exception of the Fragilaria suspension
and the auxiliary dam water, the amounts of haloforms pro-
duced in this series of experiments are only small and even
begin to decrease with longer reaction times.  This could be
due to the fact that the haloforms formed are bound to tthe
algal particles and to the cell fragments  (detritus) produced
by the chlorination, and are therefore not susceptible to the
pentane extraction used for the determination of the halo-
forms.  This explanation is supported by the fact that no
decrease of the haloforms is observed in the case of the

-------
                            -  132  -

siliceous alga Fragilaria,  which  is very  readily attacked by
chlorination, and  in  the  case of  the  micrpalgae predomin-
ating  in the auxiliary  dam  water  during' the investigation
period.  This could yield indications on  the treatment of
water  for drinking water  with respect to  the removal of halo-
forms  by particulate  organic  substances (detritus)  that can
be flocculated and filtered off.
  300 -i
 (pg/0
  200 '
  100
            Fragilaria
                Fig. 16
                Haloform formation as a
                function of the chlorine
                consumption time  in un-
                filtered algal cultures
                and in auxiliary  dam water
                during an algal bloom
                (300,000 individuals/ml)
               10
15
20
25 (h)
               Reaction time
To give a general view of the situation, we  compared  (see
Table 1) the production of organic chlorine  compounds  in
unfiltered algal samples with the.production of  organic
chlorine compounds in similar algal  samples  that had under-
gone membrane filtration. The last 'two  columns on the  right  of
this table contain the percentage TOC1  and the proportion  of halo-
forms after 20 h of chlorine reaction time   in the filtered
samples, referred to the TOC1 and halo-form content in  the
corresponding unfiltered samples.  While the TOCl-formation
potential in the membrane-filtered samples is lowered  ^y
about 50%, the haloform-formation potential  in the membrane-
filtered samples is several times that  in the unfiltered
samples.  Fragilaria is again an exception;  here there is  a
decrease to about 30%.

-------
                           - 1.33,-
 TABLE 1   TOC1 and haloform-formation potential (2O h reaction
          time)  in algal suspensions in comparison with the
          corresponding membrane filtrates (0.45

Sample
Fragilaria C14.6)
Fragilaria < 3.7}
Ps&ucianabaena
Carfceria
Pander ins
Auxiliary dam
(6.7.78)

Species
of
alga
Siliceous alga
Siliceous alga
Blue alga
Green alga
Green alga
Mikroalgae
unf Altered nfejubrane-flltered
POC
mj/I
57
62
40
63
57

TOCI
W/i
265
166
400
300
290
292
Haiof.
JI9/I
174
192
22
18
6
106
DOC
mg/I
(U)
2.1
(11)
5.0
6.5
2,9
TOCI
»/'
165.
87
173
155
165
151
Halof.
W/i
30
32
106
92
44
197
TOCI
8/aunf.
62
52
43
52
57
52
Haiof.
•fount
36
27
480
510
730
186
These figures show how different the effects of chlorination
on the production of organic chlorine compounds can be with
different algal species.  About 0.5 - 1% of TOCI was produced
with respect to the particulate organic carbon, and about 10%
with respect to the DOC.  The proportion of haloforms in the
TOCI in the membrane-filtered alga waters varied quite con-
siderably from alga to alga (about 20 - 60%), and in these
experiments had a mean value • of ,40%.  This high proportion
of haloforms in the TOCI, produced by chlorination of
membrane-filtered alga waters., is in accord with the
relevant results for water samples from the Wahnbach auxil-
iary dam, the dam itself, and the filtrate from the phos-
phorus elimination plant., -

-------
                           -  134  -

Summary'
From the time variation of the residual content of total and
free active chlorine conclusions may be drawn about,the
content of organic chlorine-consuming substances in a water.
To the water sample to be evaluated a sufficient amount of
chlorine is added, appropriate to the content of organic
substances present in the sample, expressed as the DOC. With
the aid of an automatic analyzer the concentrations of total
and free active chlorine were determined over a period of
20 - 24 h by continual measurements using the DPD method.
If the chlorine content so obtained is plotted semi-
logarithmically against time, the chlorine consumption
course can be represented by two superimposed first-order
                                1      ?
reactions whose rate constants K  and K^ are in the ranges
of 10   and 10   min  .  The  spontaneous chlorine con-
sumption, which takes place immediately after the chlorine
has been added, and the time variation of which cannot be
measured by the procedure used, is not considered here.

                    1      2
The rate constants K  and K  obtained in this way describe
the course of the chlorine consumption by the water concerned
and permit a characterization of its loading with organic
chlorine-consuming substances.  Inorganic chlorine-consuming
substances, and in particular ammonium ions, did not inter-
fere in the concentration ranges normally encountered in
surface waters.  With the aid of continual measurements of
the chlorine consumption, unloaded waters, for example, can
be clearly distinguished from anthropogenically or bio-
genically loaded surface waters, the algal substances present
in these waters as a result of widely occurring bioprocesses
also being covered.

Experiments were carried out to establish to what extent the
rate constants change in dependence on the fixed C12 : C ratio
in the range of 1 - 3 with increasing DOC concentrations.  It
was found that the rate constant of the first fast reaction

-------
                           - 135 -

(K )  for Cl- :  C ratios (concentration ratios) of 2 or 3 ,in , ,
the region 1 - 4 mg DOC/1 is more or less constant, while
the rate constants for the subsequent slow chlorine con-
           2
sumption (K )  is very strongly dependent on the initial
C12 : C ratio and is only constant for C12 : C ratios greater
than 2.

In the light of this knowledge reaction rate constants
 12                     '
K  and K  were obtained for water from the auxiliary dam of
the Wahnbach dam, for the filtrate from the phosphorus-
elimination plant, for water from the Wahnbach dam, and for
Rhine water (period of June to August 1978).  Clear differ-
                                        1      2
ences were found between the constants K  and K  in these
                       12
cases.  The constants K  and K  are proportional to the UV
extinction at 280 nm measured in each case, showing that
the chlorine consumption is due above all to the substances
responsible for the UV absorption.   From the proportionality
between UV extinction as a parameter for the description of
the "precursor concentration" on the one hand and the rate
           1      2
constants K  and K  on the other it follows that a propor-
tionality must also exist with the organic chlorine compounds
formed as the final reaction products — TOC1 and haloforms.
This relationship could be established both for humic acids
isolated from the auxiliary dam and for the various waters
of our dam system.  In exactly the same way, a connection
exists between the rate of the chlorine-consumption reaction
and the concentration of organic chlorine compounds formed.

If chlorine is allowed to react in the described manner with
unfiltered algal suspensions of Fragilaria as a represent-
ative of siliceous algae, Carteria as a representative of
the green algae, and Pseudanabaena as a representative of
the blue algae, then over a period of a few hours a very
rapid increase of the TOCl concentration is observed, which
approaches a limiting value in 12 - 24 h.  Particularly
Pragilaria, which as a siliceous alga is sensitive to chlorine,

-------
                          • - 136 -


shows a very rapid rise in the TOCl concentration within the
first few hours of reaction.  If, on the other hand, the algal
suspensions are membrane-filtered before the chlorine has been
introduced, the final TOCl concentrations are only half as
high.  Accordingly, in the practice of water treatment it
must be considered that treatment -of a raw water rich in
algae with large amounts of chlorine (preliminary and break-
point chlorination) leads to higher TOCl concentrations than
the treatment of a surface water that has already.undergone
flocculation and filtration, in which the concentration of
algae has thereby been markedly reduced.  The formation of
haloforms during the treatment of various algal suspensions
with chlorine proceeds with varying degrees of intensity,
and it should  be  particularly indicated that in the analyt-
ical determination of haloforms interference is caused by
cellular fat and oil substances, so that the haloform concen-
trations as measured appear to be low-

It has been shown that the extent of the formation of organic
chlorine compounds above a certain minimum chlorine addition
is independent of the Cl^ : C-ratio if .a sufficiently long
reaction time is allowed.  In practice this time is usually
provided by the transport of the water to the consumer.

-------
                          - 137 -.
(1)   BERNHARDT,  H.,  WILHELMS,. A.
     Der EinfluB algenbiirtiger brgahischer Verbindungen auf
     den FlockungsprozeB bei-der Trinkwasseraufbereitung
     Organische  Verunreinigungen in der Umwelt - Erkennen,
     Bewerten, Vermindern, Erich Schmidt Verlag, Berlin
     (1978)  112-146        .     •   -

(2)   BERNHARDT,  H.,  HOYER, 0., SCH-ELL, H.
     Chlorzehrung als Parameter zur Beurteilung der Qualitat
     eines Talsperrenwassers
     _2  (1978)  (im Druck)

(3)   MAIER,  D.,  MACKLE,  H.
     Wirkung von Chlor auf natiirliche und ozonte organische
     Wasserinhaltsstoffe   •
     Vom Wasser  47   (1976), 379 & 397

(4)   SCHELL, H., HOYER,  O;, .BERGER, M.
     Bestinimung  des  freien, und des gesamten, wirksamen Chlors
     mit dem Auto-Analyzer nach der DPD-Methode (Deutsche
     EinheitS-Verfahren  ,G 4)
     Z.  f. Wasser- und Abwasser-Forschung (im Druck)

(5)   EBERLE, S.H., HOYER,,O., KNOBEL, K.P.,  v. HODENBERG, S.
     Analytische und praparative Abtrennung organischer
     Sauren aus  Wasser mittels Trioktylamin
     Kernforschungszentrum Karlsruhe KfK 2529 UF (1977)

(6)   EBERLE, S.H., KNOBEL, K.P., V. HODENBERG, S.
     Untersuchungen  iiber den Einsatz der Diafiltration zur
     Analyse der organischen Substanzen des Wassers
     Kernforschungszentrum Karlsruhe KfK 2695 UF (1978)
     (im Druck)                  •

(7)   BERNHARDT,  H.,  CLASEN, J.,  HOYER, O., WILHELMS, A.
     Untersuchungen  zur  Oligotrophierung der Wahnbachtal-
     sperre - Ermittlung der derzeitigen Situation
     DVGW-Schriftenreihe Wasser (1978)  (im Druck)

(8)   WO'LFEL, P., SONTHEIMER,  H.
     Ein neues Verfahren zur Bestimmung von organisch
     gebundenem  Kohlenstoff im Wasser durch photochemische
     Oxidation
     Vom Wasser  43  (1974), 315-325

-------
                           - 138 -


 THE PRACTICE OF CHLORINATION OP DRINKING WATER
 H.  Lamblin
'In connection with the lectures during this session I should
 like, in the following to make some remarks on the selection
 of the chlorination agents and on the moment of the addition
 of these agents during the treatment.

 1.   Chlorination as a stage in the treatment of drinking
      water has been valued highly for a considerable time.

 2.   However, in more recent times analytical advances have
      directed the attention to by-products possibly exerting
      harmful effects.

 3.   A new philosophy is now arising in connection with
      chlorination.

      -  One alternative to chlorination is a method that has
         already been very extensively investigated, i.e. the
         use of a new or already familiar chemical treatment
         agent that has sufficiently strong oxidizing and dis-
         infecting properties, an effect that does not dim-
         inish with time and that eliminates aramoniacal
         nitrogen while at the same time giving harmless by-
         products .

      -  A second way consists in first chlorinating actively
         pre-clarified water, i.e. water that essentially
         contains no further substances that could lead to the
         formation of undesirable by-products.  A biological
         treatment prior to the chlorination can aid the puri-
         fication of the water still further.

      -  There is,  however, a third way, which represents a

-------
                          -  139  -
        compromise.  This consists in establishing what are
        the most suitable  points for the addition and which
        chlorination agents are most suitable for the existing
        treatment plants.  The merit of this third variant
        lies in its possible rapid realization.

        A number of external requirements that the water-
        treatment engineer must satisfy are already familiar:

        a)  the chemical elimination of the ammonia nitrogen
            may only take place after the elimination of the
            halomethane precursors,

        b)  the bactericidal action and the oxidation of the
            organic substances are clearly stronger when the
            water has been clarified beforehand,

        c)  certain organic substances not oxidized by chlor-
            ination can be made oxidizable either by an ozone
            treatment or by biological modification by passage
            through activated carbon filters or through other
            materials.

        d)  An after-chlorination may be necessary to maintain
            a good biological stability of the water in the
            distribution network, particularly in the case of
            large networks.

It can be assumed that good adaptation of the chlorination by
one or more of the available chlorine derivatives, depending
on the specific features of each case,  and a careful choice
of the time of addition in the individual stages of the treat-
ment plant, largely make it possible to limit the formation
of already known by-products and to ensure a high quality of
the product water.

-------
                          - 14O -


USE OP CHLORINE'BY THE NETHERLANDS WATERWORKS

A.P. Meijers
In practice chlorine is used at different places in the
purification system:

1.   transport chlorination of raw water or partially
     treated water?

2.   safety chlorination at the end of the purification
     process in order to avoid bacterial growth in the
     distribution system;

3.   breakpoint chlorination, most often the first puri-
     fication step, used for the oxidation of ammonia,
     some organics and finally disinfection;

4.   process chlorination in order to avoid disturbances
     during the purification processes by biological
     activity?                                s

5.   finally, chlorine is used for the oxidation of
     iron II to iron III as a coagulant.

Except in the last application, chlorine is always used for
disinfection, which process must indeed be considered as the
most important step in drinking water purification.

In order to be informed about the use of chlorine, a
questionnaire was sent to a number of selected Dutch water-
works in April of last year. (Ref.(I) Problematiek haloformen
Meded., nr. 57, KIWA, mei 1978) Prom the results of the
questionnaire it was concluded that all surface-water pro-
cessing waterworks use chlorine to some extent. The ground-

-------
                           -  141-
waterworks (60 %.) only use chlorine, incidentally. Transport
chlorination was used by three big waterworks serving
Amsterdam, Rotterdam, The Hague and North Holland.

In these cases the water is chlorinated after partial
purification with 2 to 4 g/m  of chlorine and trans-
ported for about 40 kilometers for additional purifi-
cation. In the summer more chlorine is needed for this
purpose than in the winter.

Breakpoint chlorination is used by several waterworks,
for instance in the Berenplaat works at Rotterdam.

As the surface-water processing waterworks have safety
chlorination at the end at a level of O.5 g/m , not only
chlorine gas is used but also sodium chlorite, and at
some places chlorine dioxide.

Furthermore, it was clear that surface-water processing
waterworks, which include artificial recharge in the dunes
as a purification step, do not use breakpoint chlorination.

However, before artificial recharge in the dunes, transport
chlorination took place.

Nevertheless, there are still four waterworks processing
surface water not taken from the river Rhine nor the river
Maas, which do not add chlorine for transportation nor for
breakpoint chlorination.

In summary, in one third of the produced water in the
Netherlands transport chlorination was included  (8OO tons),
1O % to 15 % was treated by breakpoint chlorination  (65O tons)
For iron oxidation 43O tons of chlorine were used, and for
safety chlorination for all waterworks only 2OO tons was used.

-------
                            142 -
Thus most of the chlorine is used for transport- and, for
breakpoint chlorination at only a limited number of large
waterworks. Therefore, the aim in the Netherlands, in order
to limit the use of chlorine, is to search for possible
alternatives in these cases.

This includes especially a better treatment before trans-
portation so that the use of chlorine will not be necessary,
removal of ammonia by biological filtration in order to
avoid breakpoint chlorination, no use of Fe II as a coagu-
lant, and finally the use of an alternative disinfectant.

However, all of these measures will involve considerable
consequences for the waterworks in question.

-------
                           -  143  -


THE PRACTICE OF CHLORINATION OF DRINKING WATER

G. Uhlig
 The  practical  engineer  often conies  across  an apparently sur-
 prising  phenomena  concerning the  chlorine  requirement  and  the
 chlorine consumption, of raw waters,  when  the treatment consists
 of a biologically  working activated carbon filtration.  This
 effects  the  so-called nitrification process, i.e.  a  micro-
 biological oxidation of the  ammonia nitrogen to  nitrate
 nitrogen.  Stoichiometrically,  2  1/2 moles of oxygen per mole
 of ammonium  or 3.6 mg of oxygen per aig of  ammonium are necessary
 for.  this purpose.   This has  the following  consequences in
 chlorination practice:

1.   If the oxygen content of the raw water  is insufficient,
the nitrification is not completed, proceeding only to  the
stage of nitrite, which  (in  addition to the  breakthrough of
ammonium) gives rise 'to  a particularly high chlorine consump-
                      •.-
tion in accordance with the  reaction

                      NO Z +  HOC! 	>• NO^ + HC1.

2.  Even when  the oxygen content of the raw  water remains
the same, an activated carbon filter must sometimes be
temporarily shut down for operational reasons.  After a
few hours the  biology of the filter is upset owing to the
lack of oxygen and the result is a sudden rise in. ammonium
and nitrite when the filter  resumes" operation.  After the
shutdown of an activated earbon filter for only 48 h we
measured a nitrite content of 1.3 mg/1.

On account of the potential danger of the production of
the extremely carcinogenie nitrosamines, it  is advisable
in such cases to reconnect the filter to the drinking

-------
                           - 144 -
water supply only under laboratory control after back-washing
and drainage of a suitable amount of first filtrate.  This
situation is otherwise signalled by a sudden rise in the
chlorine consumption resulting in disturbed chlorine measure-
ments with the same infeed.

Special problems inevitably arise when, for example, the shut-
down of a whole activated carbon filter plant consisting of
many units becomes necessary for operational reasons.  Con-
siderable amounts of first filtrate may have to be discarded
if it is not possible to continue operating the filter at
least with a minimum throughput of oxygen-rich water in for-
ward or back motion.

3.   Even after the filters have been recharged with fresh or
reactivated carbon, it can take many days before the biology
on the carbon develops its full activity.  Here too the out-
come is a strongly elevated chlorine consumption of the water
if such filters are prematurely connected to the drinking
water network.

4.   In addition,the function of some of the equipment for
continuous chlorine indication is strongly pH-dependent, and
at pH above about 7.5 free chlorine can no longer be indicated
unless an acid or buffer solution is added continuously to the
water.

However, before filters freshly charged with reactivated
carbon are put in operation, initial pH levels of 10-12 can
still be measured, presumably caused by oxides and carbonates
formed during reactivation of the carbon, depending, on the
salt content of the water with which the carbon was previously
wetted/ from the cations of these salts.

-------
                          - 145 -
FORMATION OF NON-POLAR ORGANO-CHLORO COMPOUNDS AS
BYPRODUCTS OF CHLORINATION

A.A. Stevens
The Conference Committee has assigned to me the task
of reviewing the formation of "non-polar" byproducts
of chlorination of drinking water. For the purposes
of this paper, the term "non-polar" applies to that
group of individual comppunds that can be separated
from dilute aqueous solution by solvent extraction or
gas stripping, i.e.  compounds of relatively low water
solubility. The final method of analysis is always gas
phase chromotography, further restricting this dis-
cussion to compounds volatile at those temperatures.

I.  Early experience

Before the 197O's little was known about the formation
of individual halogen substituted organic compounds
during chlorination for drinking water disinfection.
Knowledge and consideration of byproducts was limited
to the recognition of halogen substitution on nitro-
genous compounds as possible contributors to "com-
bined chlorine" (chloramines)   and chlorophenolic by-
products as causes of tastes and odors in some problem
supplies.

II.  Trihalomethanes

Not until modern analytical techniques were applied to
finished drinking water and companion raw water samples
did the list of known byproducts of chlorination begin
to grow. In December of 1974, independently, Rook (1)
and Beliar, et al. (2) reported the formation of chloro-

-------
                          -  146  -
form and other chlorine- and bromine-substitudecl tri-
halomethanes during drinking water treatment as a
direct result of the chlorination-disinfection practice.
Partly because of these discoveries, the United States
Environmental Protection Agency  (USEPA) undertook a
survey of 8O selected cities to measure the concentra-
tions of six halogenated compounds in raw and finished
water (3). Those six included the four trihalomethanes
(chloroform, bromodichloromethane, dibromochloromethane,
bromoform) suspected of being formed during chlorination,
plus carbon tetrachloride and 1,2-dichloroethane, known
contaminants at some locations, but not necessarily
formed on chlorination. During this National Organics
Reconnaissance Survey  (NORS) the occurrence of trihalo-
methanes in finished drinking water was demonstrated to
be widespread and a direct result of the chlorination
practice. No hard evidence was found in this regard
rfith respect to 1,2-dichloroethane or carbon tetra-
Dhloride. More recent surveys conducted by USEPA and
others have not resulted in a change of this conclusion
regarding the haloforms, although CC1, has since been
found to be an occasional significant contaminant of
ci2.
Based on the survey results, a theoretical finished
water with the median concentration of each compound
would contain about 21 yg/i. of chloroform, 6 yg/.l of
bromodichloromethane, 1.2 yg/1 of dibromochloromethane,
and an amount less than the detection limit for the
method used of bromoform (Fig. 1). Although most of
the finished waters tested demonstrated this decreasing
order of concentration, this was not always the case.
The finished water at one location had a chloroform
concentration of only 12 yg/1, but a bromoform concen-
tration of 92 pg/1. This high concentration of bromo-
form was suspected to reflect a relatively high bromide

-------
                           - 147 -
concentration in the raw water  (see  below).  Even,though
more recent information indicates  a  preponderance of yet
unidentified organic-substituted halogen,  measured as
"organic halogen" in some  form  (4),  the trihalomethanes are
present in the highest concentrations of non-polar species
resulting from chlorination  of  drinking water individual-
ly identified to date  (September 1978).
  300
  1OO
 o» 50
 O
ui
U
8
UJ
3
   10
   1.O-
   O.5
   O.1
        o
     2 5 1O 3O SO 7O 9O   99
    PERCENT EQUAL TO OR LESS
    THAN GIVEN CONCENTRATION
Fig 1  Frequency distribution
       of txihalomethane data.
       NORS
Because  of findings concerning the carcinogenicity  (5)
of  chloroform the USEPA has proposed an interim Primary
Regulation for trihalomethanes in consumers' drinking
water  of 0.1O mg/1 total trihalomethanes  (CHC1,,, CHCl~Br,
                                               •5      £*
CHBr-Cl,  and CHBrO.  At many water utilities, specific
new or improved treatments will be required to reduce
existing concentrations of trihalomethanes to meet  this
standard. Indeed, the continued heavy use of chlorine

-------
                             148  -
for drinking water disinfection is now being questioned
in the USA.
Factors influencing trihalomethane formation
The formation of trihalomethanes during chlorination of
drinking water now seems to be well accepted to result
from a complicated mechanism of attack by aqueous halo-
gen species on natural aquatic humic substances (humic
and fulvic acids) and not usually significant from
sources of industrial water pollution (1,6,7,8).
Design of the most effective treatment strategy depends
on a good knowledge of factors influencing trihalo-
methane formation. Two factors, however, that have a
strong influence on trihalomethane  joncentrations over
which the water treatment plant ••\':-..,rator has little or
no control under most circumstance-3 are temperature and
Br  or I  concentration.
Temperature
Figure 2 clearly demonstrates the positive effect of
increasing temperature on trihalomethane formation upon
chlorination of Ohio River water in the laboratory (7).
A corresponding seasonal variation is noticed at a water
utility using that same source and has been shown to
be largely a temperature effect  (1O). Thus treatment
problems become more acute during seasons of higher
ambient temperature causing higher water temperatures
during treatment and distribution.

-------
                           - 149  -
          40  60   BO
            TIME (hreS
                             Fig., 2
                             Chloroform production at three
                             temperatures raw^ water
                             10 mg/1 chlorine dose; pH 7
Bromide and iodide concentration
                   jf
Bromide'and iodide ions are oxidized by aqueous chlorine
to species capable of participating in organic substitu-
tion reactions resulting in the formation of pure-and
mixed halogen trihalomethanes. Bunn  et al.  (1O) first
confirmed one of the suspicions of Rook (1) that this
could occur in aqueous systems when they chlorinated
Missouri River water in the presence of added fluoride,
bromide,  and iodide and observed the formation of all
ten possible chlorine, bromine,^and iodine containing
pure- and mixed halogen trihalomethanes. On a theoreti-
cal basis, fluorine substitution was not expected and
was not observed. To date, at least six of these
species have been found in finished drinking water
(chloroform, bromodichloromethane, dibromochloromethane,
bromoform, dichloroiodomethane, and bromochloroiodo-
methane)   (11).

-------
                          -  150 -
 p
                           282 M equiv. CI2 dose per liter
             2O    30    40    50    60               313
                      u mols Br~ added

 Fig.  3 Trihalomethanes formed by reaction of humic acid
        with aqueous chlorine in the presence of varying
        bromide ion
Figure 3 illustrates-the  results  of  work  conducted in
our laboratory on the effect  of added  bromide on the
ratio of trihalomethanes  produced during  reaction of
aqueous chlorine with humic acids. Note that bromine
substitution is favored over  chlorine  even though
chlorine is in large excess compared with the initial
bromide. Additionally, the total  molar yield of tri-
halomethanes increases with increasing bromine substitu-
tion. This was also observed  when pure aqueous bromine
was reacted with the humic acid under  the same condi-
tions as aqueous chlorine. Thus,  bromine  competes more
effectively than chlorine for  active  sites on the humic
acid precursor molecule,  perhaps  mechanistically by way
of faster substitution reaction rate.  This effect is
so pronounced as to dramatically  increase total haloform
yields where bromide is present.  Indeed,  similar in-
creases in total haloform yield have been reported to

-------
                           - 151 -

occur  on  chlorination of--a bromide spiked  natural water
 (11) and more importantly at a water treatment  plant in
the USA where sea water intrusion was temporarily respons-
ible for increases in bromides (12)  (Fig. 4). Thus, much
more complete control of trihalomethane precursor, as
one method of meeting proposed USEPA drinking water
standards,  is necessary when significant  concentrations
of bromide are present in the source water.
 180
 160
 140
 120

IflOO
  80
  60
  40
  20
 LEGEND
> 
-------
                          - 152- -
             2O
30   .40    50   60
   REACTION TIME (hrs)
                                      70
                                           8O
                                                9O
Fig. 5 Effect of pH on chloroform production,  settled
       water 25°C, 10 mg/1 chlorine dose
     The increase of trihalomethane  formation  rate with
pH was expected because the classical haloform reac-
tion is base catalyzed; however, this explanation is
likely to be an oversimplification where  rather complex
humic acid structures are involved.  Simple methyl ketones,
models for the haloform reaction, have been  shown to
react too slowly to account for trihalomethane formation .
under most drinking water conditions, suggesting a
different reaction mechanism  (7). Christman  once suggested
a simple "opening up" of the humic acid molecule because
of mutual charge repulsion at high pH increasing the
availability of more reactive sites"  on that  molecule  as
a possible cause of the influence of high pH on reaction
rate  (Personal communication)." ;

-------
                           - 153 -

Characteristics and Concentration of  Precursors
In artificial systems, increasing  the  concentration of
humic acid precursor in  the presence  of  excess chlorine
with otherwise constant  reaction  conditions  causes halo-
form yields to increase  in direct proportion to the
humic acid dose {Pig. 6)  (7).  Prom supply to supply,  how-
ever} only crude relationships  have been  found between
organic carbon concentrations, and trihalomethane yields
(3). Similar effects have been noted  upon treatment,
Further, rate curves seem to  take on  distinctly different
shapes depending on the  source of precursor  substances.
The work of Rook  (8) shows  the reaction  of fulvic acid
solutions to be characteristic of m-dihydroxyphenyl
moieties  (e.g. resorcinol)  in  that the reaction is near-
ly complete at near neutral pH"in less than  two hours
(Pig. 7).
  0.3- •
             20
                 30
                      4O    50   60
                         TIME (hrs)
                                    7O
80
     90
         100
Fig. 6 Effect of humic acid concentration on trihalomethane
                             .o
       production, pH 6.7; 25 C; 10 mg/1 chlorine dose

-------
                            - 154 -
o
| 200-
o
to
O
cc
| 150-
"wlOO-
0
£ (
"a
6 50-
E


^ RESOBCINOL __^< Q
—
0
FULVIC A^D__ ^
«•«• •**** ~~
!J> x*"
r

2
-4O «
'5
i-
-3O O
e
i *•
fc rv
-20 w®
o
X -g
-10 i i
El
          10
           20    30    4O     SO  T  11O    120    130
Fig. 7 Reaction of model precusors with aqueous chlorine
      so   ao
             M  tO   SO  60
              REACTION TIME (hrt)
Fig.
j8 Comparison of humic acid,  raw water reaction rates
  at similar NPTOC concentrations, 10 mg/1 Chlorine dose

-------
                           -  155  -


Quite a different characteristic curve is observed with
Ohio River water precursor and a different source of
humic acid under similar conditions where the reaction
takes place relatively slowly over a period of many
days (Fig. 8) (7). The probable differences in pre-
cursors at different locations has been further demon-
strated in work at the EPA Cincinnati laboratory where,
as expected, treatment with permanganate at low dosages
was nearly 1OO percent effective in preventing the for-
mation of trihalomethanes on chlorination of resorcinol
and m-dihydroxybenzoic acid solutions, yet permanganate
was only marginally (1O-2O%) effective in reducing the
ability of Ohio River precursors to form trihalome-
thanes upon subsequent chlorination.

Additionally, work at the Cincinnati laboratory has
shown there to be only a slight  influence on trihalo-
methane formation rate  (or yield) of increasing chlorine
dose (beyond demand) where "precursor" is kept con-
stant  (Fig. 9). Both similar and contrary results have
been reported by others while working with different
sources of precursors (6, personal communication).
The above serves only to indicate that although pre-
cursor materials from various supplies may be of large-
ly natural origin, the composition of that material is
likely to be different, depending on the type of supply
involved and the source of precursors in the water shed.
Considerably more work is needed, therefore, to under-
stand the complex mechanisms of trihalomethane forma-
tion during water chlorination and to determine whether .
water treatment strategies for control of THM's could
vary significantly among these various supplies.

-------
                           - 156 -
 0.6
 0.5-
§0.4
t>
I 0.3
a
£ 0.2
Chlorine Dose;
   6.0 mg/L *
   4.0 mg/L .-
   3.0 mg/L a
                                                     SJO
                                                4.O2
                                                3.O jj
                                                  O
                                                  •
                                                  2
                                                2.O •
        10
             20
                  30
                  4O    SO    6O
                  Tim* (Hrs)
                                      7O
                                           80
                                                90
Fig. 9 Effect of chlorine dose on trihalomethane formation
III.  Other  identified apparent products of chlorina-
      tion/treatment
As mentioned earlier,  trihalomethanes represent the most
important group  of individual identified halogenated
species  from a concentration standpoint identified in
finished drinking  water and resulting from chlorina-
tion practice. Although the mechanisms for trihalome-
thane formation  are not well understood, many of the
conditions favoring or inhibiting trihalomethane for-
mation have  been established. Other non-polar compounds
have been detected in  finished water at the ng-ug-/l
level that were  not detectable in the source water or
present  in lower concentrations. Most of the sources of
these are even less well understood.
At least 19  non-trihalomethane halogenated volatile
compounds have been shown by Rook  (8)  (Rotterdam Sto-
rage Reservoir)"and more by Stieglitz, et al.,  (14)

-------
                          - 157 -


(Rhine River Bank Filtrate) to be formed at low con-
centrations upon chlorination. Rook speculates on a
possible pathway to explain the formation of some of
the observed byproducts in a way related to his pro-
posed mechanism for haloform formation from m-dihydroxy-
phenyl moieties. Stieglitz suggests no mechanism.
Coleman et al. (15) reported the co-presence of chloro-
picrin, chlorobenzene, a chlorotoluene isomer and a
chloroxylene isomer with their respective logical pre-
cursors, nitromethane, benzene, toluene, and m-xylene,
in finished chlorinated tap water. All of the above pre-
cursors but benzene were shown to be reactive with
aqueous chlorine to form the expected products. More
recently, chloroacetonitrile derivatives have been ob-
served in a finished tap water as a result of work at
the Cincinnati laboratory. Milligram per liter concen-
trations of acetonitrile could not be made to react
with chlorine under realistic reaction conditions to
form detectable chlorinated derivatives, however.
Further, even simple aromatic hydrocarbons have been
observed in some studies to be more prevalent or in
higher concentrations in finished tap water than in the
respective raw source water (16,17).
Considerable effort lies ahead to determine mechanisms
for formation of these apparent byproducts of chlorina-
tion (or possibly other treatment in the latter cases)
that in most cases seem to defy straightforward explana-
tions .

-------
                          - -158 -

IV.  Summary

Modern analytical techniques have expanded our knowledge
of the formation,of unwanted by-products during chlori-
nation of drinking water. Of the non-polar fraction, tri-
halomethanes typically are formed in the highest concen-
trations, and much is now known about factors influencing
their formation. Other chlorinated and non-chlorinated
non-polar apparent by-products have been observed, but
little is known about their sources.

-------
                           -.159 -
 (1)   ROOK,  J.J.
      Formation of Haloforms During Chlorination of Natural
      Waters
      Water  Treatm. and Exam.  23 (1974), 2, 234

 (2)   BELLAR,  T.A., LICHTENBERG, J.J., KRONER, R.C.
      The Occurrence of. Organohalides in Chlorinated
      .Drinking Water
      J.  AWWA  66  (1974) , 11 , 7O3

 (3)   SYMONS,  J.M., BELLAR, T.A., CARSWELL, J.K.,
      DeMARCO, J., KROPP,  K.L.,  ROBECK, G.G., SEEGER, D.R.,
      SLOCUM,  C.J., SMITH, B.L., STEVENS, A.A.
      National Organics Reconnaissance Survey for Halogenated
      Organics in Drinking Water
      J.  AWWA  67. (1975), 11, 634

 (4)   KUHN,  W.,  SONTHEIMER, H.,  KURZ, R.
      Use of Ozone and Chlorine  in Waterworks in the Federal
      Republic of Germany                                   '
      Ozone/Chlorine Dioxide Oxidation Products of Organic
      Materials
      Proceedings of Conference, International Ozone Institute,
      Cleveland,  Ohio (1978),  426-442

 (5)   -
      Report on the Carcinogenesis Bioassay of Chloroform
      National Cancer Institute, Div. of Cancer Cause and
      Prevention, Washington,'D.C.  (1976)

 (6)   ROOK,  J.J.
      Haloforms in Drinking Water
      J.  AWWA  6_8  (1976), 3, 168

 (7)   STEVENS, A.A., SLOCUM, C.J.,  SEEGER, D.R., ROBECK,  G.G.
      Chlorination of Organics in Drinking Water
      J.  AWWA  68  (1976) ,11,615

 (8)   ROOK,  J.J.
      Chlorination Reactions of  Fulvic Acids in Natural Waters
      Environmental Science and  Technology 11 (1977), 5,  478

 (9)   LOVE,  O.T.
      Drinking Water Research Div., Municipal Environmental
      Research Laboratory, US EPA,  Cincinnati, Ohio
      Unpublished data

(1O)   BUNN,  W.W., HAAS,  B.B., DEANE,  E.R., KLEOPFER, R.D.
      Formation of Trihalomethanes  by Chlorination of
      Surface  Water
      Environmental Letters IP  (1975), 3, 2O5

-------
                         - 160 -
(11)   KLEOPFER,  R.D.
      Analysis of Drinking Water for Organic Compounds
      Identification  and Analysis of Organic Pollutants in
      Water,  Ann Arbor Science,  Ann Arbor,  Michigan (1976), 399

(12)   LANGE,  A., KAWCRYNSKI, -E.
      Contra Costa County Water  District Experience
      Presented at the California - Nevada  Section- AWWA
      Seminar on Organics in  Domestic Water Supplies,
      Palo Alto, CA,  USA, April  12 (1978)

(13)   SYMONS, J.M., STEVENS,  A.A.
      Physical-Chemical Pretreatment for the Removal of
      Precursors
      Presented at Conference on Oxidation  Techniques  in
      Drinking Water  Treatment at Karlsruhe, Federal Republic
      of Germany, Sept. 9-13  (1978)

(14)   STIEGLITZ, L.,  ROTH, W. , KtlHN, W., LEGER,  W,
      The Behavior of Organohalides in the  Treatment of
      Drinking Water
      Vom Wasser £7 (1976), 347

(15)   COLEMAN, W.E.,  LINGG, R.D., MELTON, R.G.,  KOPFLER,  F.C.
      The Occurrence  of Volatile Organics  in Five Drinking
      Water Supplies  Using Gas Chromatography/Mass  Spectro-
      metry
      Identification  and Analysis of Organic Pollutants in
      Water,  Ann Arbor Science,  Ann Arbor,  Michigan (1976) ,
      305

(16)   SEEGER, D.R., SLOCUM, C.J., STEVENS,  A.A.
      GC/MS Analysis  of Purgeable Contaminants  in Source and
      Finished Drinking Water
      Proceedings of  26th Annual Conference on  Mass Spetro-
      metry and Allied Topics, held in St.  ;Louis, Missouri,
      May 28  - June 2 (1978)

(17)   BRASS,  H.J., FEIGE, M.A.,  HALLORAN, T., MELLO, J.W.,
      MUNCH,  D., THOMAS,  R.F.
      The National Organic Monitoring  Survey:  Samplings and
      Analysis for Purgeable  Organic Compounds
      Drinking Water  Quality  Enhancement Through Source
      Protection, Ann Arbor Science, Ann Arbor,  Michigan
      (1977), 393

-------
                         - 161 -

FORMATION AND BEHAVIOUR OF POLAR ORGANIC CHLORINE COMPOUNDS

W. Kiihn and R. Sander
In the previous report, Dr. Stevens reported on non-polar
organic chlorine compounds which can be formed in the chlori-
nation of waters. The resolution of this subject into two
components, i.e. into non-polar and polar chlorinated products,
is not founded on their possible hygienic or toxicological
differences but rather on the very different methods used
for their analytical detection  (1,2).

This class of compounds has recently attracted considerable
interest not only because of its toxicity but because its
study was first made possible by modern analytical methods.
The phrase "a water is only as good as analysis permits" is
particularly appropriate to the analysis of organics in water.

For this reason I should like to start with a careful con-
sideration  of the analytical treatment of this class of
compounds.

As shown in Table 1 , the treatment of organic chlorine com-
pounds, which as a rule are present in microgram amounts,
can be divided into an enrichment stage, separation into
individual substances, and the actual determination. In
practice, all polar organic chlorine compounds present analy-
tical difficulties. In contrast to the usually low-molecular,
non-polar, volatile compounds, on which the previous speaker
reported, polar compounds are usually high-molecular, less
volatile, and therefore more difficult to deal with. However,
if a group of substances resists individual substance analysis,
it is legitimate and sensible, and not only in water chemistry,
to treat these substances by a general or group method. The

-------
                         - 162 -     •


method developed  at  the Engler-Bxmte-Institirte for  the treat-
ment of all the organic chlorine  compounds  (TOCli. total

organic chlorine) begins with an  adsorptive enrichment,
followed by mineralization in pyrohydrolysis and subse-
quent detection of the now easily analysed chlorine  (3-5).
    TABLE  1
Treatment of halogen compounds in water
     Enrichment;
      (preliminary
     separation)
     Separation:
     Determination;
              Liquid-liquid extraction

                discontinuous
                continuous (stages)

              Blowing out
                static (headspace)
                dynamic (concentration)

              Adsorption (elution)

                batch test
                column

              Gas chromatography

                packed columns
                capillary columns .

              Mass spectrometer
              Conductivity detector
              Microcoulometer.
              Electron capture detector  (BCD)
              Plasma detector
              Neutron activation
              Pyrohydrolysis
                (+ chloride determination)
As shown in the next table, the enrichment can be divided
into the following steps:

-------
                         - 163 -
     TABLE  2  Experimental  conditions "of  the  combination
              of  adsorption and  flocculation  for TOC1
              treatment
     ADSORPTION:
     FLOCCULATION;
     FILTRATION:
     WASHING:
     FILTRATION:
s-olution
pH
powdered carbon
NaNO3
time

pH
A13+
polymer
time

blue-band


NaNO3
time
:  1-20 litres
:  <_ 3
:  100 mg/1 <_ 60 ym
:  0.01 N
:  30 min

:  6.5-7
:  5 mg/1
:  0.4 mg/1
:  ca. 5 min

:   (pressure filter)
:  0.05 N (200 ml)
:  30 min
blue-band  (pressure filter)
With this enrichment process practically all organic consti-
tuents of the water that can be adsorbed and flocculated are
enriched.  The water to be analysed is first of all treated
with solid sodium nitrate to obtain a O.01 N nitrate solution.
This reduces the undesirable adsorption of chloride. After
the addition of the active carbon in powder form, the pH is
adjusted to £ 3 with sulphuric acid.  If the carbon suspen-
sion is thoroughly stirred,the adsorption process is as a
rule completed in half an hour. Most of the supernatant water
can be easily siphoned off from the powdered carbon if the
carbon is previously flocculated.  The flocculating agent is

-------
                          -  164  -,  -


5 mg Al   per  litre,  added in the .form,of; al-uminitmi sulphate.,
For the flooculation  the water must be adjusted to pH 6.5-7
with sodium  carbonate.   To assist the flocculation, 0.4 mg/1
of partly saponified  polyacrylamide is added to the water  and
is subsequently decanted off.'  This process can be repeated
if a more complete  treatment is desired. After filtration
of the combined sludges through filter paper, the filter
cake is suspended in  2OO ml of G.O5 N sodium nitrate and
stirred for  half an hour. This removes nearly all inorganic
chloride.                         .  .

The filter cake is  then subjected to pyrohydrolysis, the
organically  bound chlorine being thus converted into inor-
ganic chloride,  as  is shown in the  following diagram.
                      PIRh-Pt Thermocouple  ( PlRh-Pt Thermocouple

                                      Quartz Wool Plug
                          Pyrohydrolysis Apparatus
                           Fig.r
This mineralization takes place1 in a current of steam and
oxygen at 90O°C  in  a tubular furnace. The chloride is then
determined titrimetrically or microcoulometrically.
By means of this pyrohydrolysis technique,the organic chlor-
ine materials can be  treated as a group. As preliminary

-------
                         - 165 -

individual investigations- have shown, the majority of the
polar compounds are high-molecular, chlorinated lignic and
humic acids .
In order to investigate on a laboratory scale all the pro-
cesses taking place during, the ..chlorination of "water,
model waters containing humic acid were treated with
chlorine water, since humic acid can be used as a starting
substance for haloforms. An example of the results of this
work is given in the following figure.
          :Gig/0
       2500-
       2000 J
       1500-
       1000
       500-
                                  TOC1
                                  Chloroform
                                  Free chlorine
                                           -1
                            40   50  ClJjpg^O
Fig .  2
        Chloroform formation,  course  of TOC1  and final
        chlorine  content  as' a  function of the initial
        chlorine  content  at a  constant reaction time
        of  23  h

-------
                          -  166  -

Here the concentrations of chloroform and TOC1 are plotted
together with the chlorine content in dependence on the
dose of chlorine? the amount of humic acid weighed in was
2O mg/1 and the pH was 6.9.  It is clear that the chlorine
content of the chloroform corresponds only to a small part
of the total organically bound chlorine. Therefore, the
effect of a chlorination cannot be evaluated solely on the
basis of the measured haloform concentrations? the TOC1
must likewise be considered  (6).
          2500-
          2000
          1500-
          lOOO-i
           500-
              0   10   20   30  40   50 HS-NaQng/b
 Fig.  3  Chloroform formation, course of TOC1, arid
         final chlorine ccntent as a function of the
         amount of humic acid weighed in, with a
         constant reaction.time of 23 h

-------
                          -  167 -

      •    -_,:,-, ;..',:' '. ',«   •••• • > •"- "• v , ' -  " .'  •?'••_••>?•• • .•,•.*.••<•;•..•:.• -\  .. :-*-<
As can be seen  from Fig. 3, at a constant amount  of chlor-
ine and increasing humic acid concentration, similar rela-
tionships are obtained for the formation of organic
chlorine  compounds.

To obtain information  on further parameters influencing
the formation of  chlorine compounds, in addition  to vary-
ing the reaction  time, we varied the amount of  chlorine,
the initial  humic acid concentration, and the  pH.'  The
relationships represented in the following illustration
were obtained.
       CHCl, Qjg/Q.
         m
      250-
      200-
      150-
      100-
                                     10
                                     20 Qj/20 HS~Na
       aa/2.o
HS-Nbi
o—o pH 9,2
     PH6.3
                                             HS-Ntt
                                            	•»•.
     4   Chloroform concentrations as  a  function... of the
        reaction time with various  starting conditions
        (numerical data in mg/1)                 ,

-------
                        -  168
In agreement with the relationships reported in the previous
lecture by Dr. Stevens, more chloroform is produced at
pH 9.2 than in the neutral range.'

In contrast to this, the formation of TOC1 is enhanced at
lower pH, as shown in the next figure.
        TOO
                             pH 9. 2
                             pH 6.9
                                     •2O  C12/2O HS-Na
                                     1O  C12/2O HS-Na
                                     2O  C12/2O HS-Na
                                     10  C12/20 'HS-Na
                                     20 Cl«/1.5 HS- Na
                            20
30Time CH)
Fig. 5   TOC1 as a function of the reaction time with
         various starting conditions  (.numerical data in mg/1)

In the experiments performed here, the haloform reaction
ended after about 24 h.
At pH 6.9 there was a clear increase in the amount of TOC1
formed. The influence of the pH on the chlorine consumption
or the TOC1 formation can be explained by the increase in
the electrophilic character of hypochlorous acid in the acid
medium.

-------
                        -  169  -  ,

 in  addition  to  these  relationships,  it was  also  of  interest
 how the  formation  of  the organic, ghlorine substances  is
 influenced by suitable pre-treatment methods   (7).  Since
 ozonization  is  regarded in waterworks operation  as  a  prac-.
 tical  possible  solution, further, experiments were performed
 after  a  preliminary ozonization..The results on  the form-
 ation  of polar  and higher-molecular  halogen compounds after
 ozonization, measured as TOC1, are shown in the  next  illus-
 tration.
         TOCl Qjg/Q
       2000-
        1500
        1000
         500
20 Cl^/20 HS-Na
      Q.6mgC\/rng C
:'     2.5   '
                                         20 Time(H)
 Fig.  6     Course  of TOCl as  a  function  of  the  reaction
            time with various  ozone  consumptions (20  mg/1
            chlorine  + 2O mg/1 HS-Na)
The diagram demonstrates that even small additions of ozone
noticeably reduce the formation of organic chlorine compounds.
In the present case an ozone addition of 2.5 mg/1 water re-
duces the TOCl formation to one-quarter of that formed in the
absence of ozone.

-------
                        -  170 -


Similar results were obtained for chloroform formation,
as shown in Pig. 7.
                             O    Q.6mg QeonfmgC
                                  2,5mg
                                        20 Time (h)
Fig. 7  Chloroform formation as a function of the reaction
        time with various ozone consumptions  (.20 mg/1 of
        chlorine + 2O mg/1 HS-Na)
In this respect,however, different authors have obtained
different results.
In practical water-works operation other effects on the
formation and behaviour of the organochlorine compounds are
of interest. Chlorine is used in large quantities especially
for the removal of ammonia in so-called break-point chlor-
ination.   For this reason the TOC1 formation in the presence
of ammonia was studied.  The results are summarized in Fig. 8.

-------
                         _ 171  -
                                               TOCl
           500n
           400-
           300-
           200-
           100i
              0
10
20
30 chlorine
  addition
Fig. 8    Chloroform and TOCl concentrations as a function
          of the initial chlorine concentration after a
          reaction time of 2O h
It can be seen clearly that below the break-point, where for
a short time until the formation of chloramines still rela-
tively little free chlorine is present, the formation of
chloroform and of TOCl is very low.

-------
                         - 172 -  "

After the break-point has been reached, however, a small   '"-
addition of chlorine is sufficient to form large amounts
of these constituents.

These results are in accord with trials in an experimental ,
plant in Stuttgart municipal works, where good results
were obtained with stepwise chlorination just up to the
break-point. In comparison with the conventional practice,
this reduced the formation of chlorine compounds by a factor
of 10.

The data on the conventional procedure, obtained from the
same experimental plant, are compiled in Table 3.
 TABLE 3  Effects of classical treatment for a river water
          (Neckar, Stuttgart) with break-point chlorination
          and activated carbon filters
Riv«f
water
Dissolved organic 5.0
carbon (DOC) mg/I
UV absofbance 1O.9
at254nm m1
Sum of haiofomis pg/1 0.4
Total organic 33
cHorimtTOCI) pg/l
After breakpoint
chkxinatkxvftoccu
lation.secHman
tatiort and filtration
4.1
8.6
50
524
Aft*r GAC
Carbon
LSS
3.1
5.0
16
364
AfMrGAC
Caftan
F300
1.6
3.6
25
296

-------
                         - 173--

After 'chlorination with 2O rag of chlorine per litre, both
the haloform concentration and the TOC1 concentration
rise , sharply.  The quantity of trihalomethanes accounts
for only 10% of the total chlorine compounds formed. This
large proportion of polar chlorine compounds, which essen-
tially still resist individual analysis, is serious, espec-
ially in view of the fact that, as can be seen from the
last two columns of the table, these compounds are also
adsorbed only with difficulty in the subsequent active
carbon filter (8).  A selectivity of the active carbon
filters is evident, since the carbon, which removes the
TOC1 less efficiently, adsorbs the trihalomethanes better
and vice-versa.

These statements are confirmed by measurements made in a
water treatment plant at the Rhine-. The results are collected
in the following table.
 TABLE4  Course of the concentration of trihalomethanes
          and of the total organically bound chlorines
          during the treatment of drinking water in a
          Rhine waterworks

River bank filtrate
Raw water after chlor-
ination (2 mg C12/1)
After filtration
After active carbon
filter
x)
CHC13
2,3
7.3
5,1
0,5
CH3rCl2
n.n.
16.5 '
lo.7
1.0
CHSr2Cl
n.n.
15.5
11*1
o.G
CHBr3
n.n.
3.o
2.4
o,2
• I TKM
2.3
42.3
29.3
2.7
TO Cl
35
—
195
55
       All  data  in mg/m
n.n. = no trace

-------
                        - 174 -

Here too,large amounts of organic chlorine compounds other -
than the haloforms are produced in chlorination with 2 mg
C12/1. However, the variation of the concentration of these
compounds in the course of tha treatment is interesting.
The chloroform, which in comparison with the other trihalo-
methanes is more soluble in water, is also less efficiently
removed by adsorption in the active carbon filter, but in general
the total amount of haloforms is better removed by adsorption
than the polar organic chlorine compounds here grouped as
TOC1.  The difficulty that these substances also present
from the point of view of treatment technology, means that
greater attention should be paid to them in water quality
control.

Summing up, it can be said that,in addition to the trihalo-
methanesr far greater amounts of highi-molecular chlorine
compounds can be produced. The compounds that can be deter-
mined by individual substance analysis represent only the
tip of the iceberg. The aim of further research must be to
learn more about the structure of these polar compounds and
about their formation reactions.

-------
                    - 175
Cl)  KtiHNY' W.% SANDER, R.
    Vorkojtimen und Bestaromung leichtfliichtiger
    Chlorkohlenwasserstoffe
    Hydrochem. hydrogeol. Mitt.' 3 (1 978) , -327-340

(2)  STIEGLITZ, L. , ROTH, W. , KtiHN, W.,'LEGER, W.
    Das Verhalten von Organohalogenverbindungen bei
    der Trinkwasseraufbereitung
  ,  Vom Wasser £7_ (1967), 347-377

(3)  KfiHN, W., SONTHEIMER, H.
    Zur analytischen Erfassung organischer Chlor-
    verbindungen mit der temperaturprogrammierten
    Pyrohydrolyse
    Vom Wasser 43 (1974), 327-341

(4)  KUHN, W.
    Untersuchungen zur Bestimmung von organischen
    Chlorverbindungen auf Aktivkohle
    Dissertation, Universitat Karlsruhe (1974)

(5)  KtiHN, W. , FUCHS, F. , SONTHEIM1R, H.
    Untersuchungen zur Bestimmung des organisch gebundenen
    Chlors mit Hilfe eines neuartigen Anreicherungsverfahrens
    Z.  f. Wasser- und Abwasser-Forschung ^ (1977), 192-194

(6)  'SANDER, R. , KtJHN, W. , SONTHEIMER, H.
    Untersuchungen zur Umsetzung von Chlor mit
    Huminsubstanzen
    Z.  f. Wasser- und Abwasser-Forschung j> (1977), 155-16O

(7)  KtJHN, W.  et al.
    Use of ozone and chlorine in water utilities in the
    Federal Republic of Germany
    J.  AWWA,  June (1978), 326-331

(8)  KtiHN, W. , FUCHS, F.
    Untersuchungen zur Bedeutung der organischen Chlor-
    verbindungen und ihrer Adsorbierbarkeit
    Vom Wasser 45 (1975), 217-232

-------
                         -  176 -


REDUCTION OF THE'CONTENT OF CHLORINE COMPOUNDS BY A TREATMENT
COMBINING PHYSICO-CHEMICAL AND BIOLOGICAL PROCESSES

J. Chedal and P. Schulhof
INTRODUCTION
The waterworks which supply Paris with drinking water, and
notably the largest one, all treat surface water.

These plants were built about twenty years ago, following the
classical pattern of physico-chemical treatment:

-  chemical pre-treatment comprising oxidizing agents,
   in this case chlorine and chlorine dioxide, and an
   adsorbing agent: powdered activated carbon;

-  coagulation;

   flocculation, decantation, rapid sand filtration;
   final addition of chlorine before injection into the
   distribution network.
Several years later a further treatment was added: ozonization
of the filtered water with a residual content of O.4 mg/1
maintained for over 1O minutes. Lastly, treatment aimed at
protecting the distribution network was instituted by means
of a combined treatment with chlorine and chlorine dioxide.

Precautions were taken at each stage of the plant construction
to ensure that the process could be adapted as time went by
with a minimum of labour, to keep pace with technical advances,
degree of river pollution, and quality objectives for the
treated water.

-------
                           - 177 -

 »foday these precautions have proved very valuable., for. many
  changes have taken place in the course of the last twenty
  years.

  In the first place, the pollution of the Seine has greatly
  increased. Although the water to be treated is collected
  upstream of Paris, the built-up area has spread and there
  has been a great deal of urban growth upstream of the
  water-collection point.  The water has become increasingly
  charged with ammonia (see Fig, 1). ,
    65   66  67  68  69  7O   71   72  73  74  75   76  77
    Fig. 1  Content of ammonia in Seine water
  It is above all extremely rich in organic matter.  During the
  second half of 1977,  for example, the mean amount  of non-
et!
  volatile organic carbon was greater than 5 mg/1, with a
  maximum of 9.6 and a  minimum of 2.8.  On the other hand,
  the water contains a  relatively small amount of organo-
  chlorine compounds and trihalomethanes.  In the course of
  1977, for example, the amount of chloroform varied between
  O and 28 pg/1, with a mean content of 6.5 yg/1.

  In parallel with this, during the last twenty years the
  requirements on the quality of the treated water have
  developed in a way that we all know.

-------
                         - ,178 -

In connection with this development, one of the first ob-
jectives to reach is discontinuation of the pre-treatment
with chlorine at the breakpoint. This is a process that
certainly eliminates ammonia, but it also leads to the
formation of a substantial amount of haloforms.
Another objective is to obtain treated water containing a
minimum of organic substances.


DESCRIPTION OF THE TESTS
A pilot plant was built to test the efficiency of the various
improvements. It comprises two lines of treatment, each capable
of processing 1O m /h, and in which the stages oif pre-treatment,
flocculation, decantation, filtration, and ozonization are
exactly the same as those in the full-size plant (Figs. 2-4).
                           Fig. 2
                           Flocculation and
                           decantation units
                           in the pilot plant

-------
                        _ 179 -
                               Fig.  3
                               Filters of the
                               pilot plant
                                Fig.  4
                                Post-ozonization
                                columns in the
                                pilot plant
During tiie various tests undertaken one line was equipped for
specific treatments, consisting of modifications or additions
as compared with the reference line. This enabled the effects
of these modifications on the water quality at all the different
stages to be determined.

The aim of the tests, whose results are reported below, was
to examine the effects of two modifications:
-  preliminary ozonization of the raw water as an oxidation
   process before coagulation and, in conjunction with this,
   discontinuation of the chlorine pre-treatment;

-------
                          - 180 -.
    filtration through activated carbon instead of sand,
Several of the filters, with  a  specific surface of  117 m  ,
were  filled with activated  carbon.  The qualities of carbon
and the gradings most suitable  for  satisfactory filtration
were  subsequently tested, and the optimum treatment parameters
were  determined. It was found,  in this respect, that the  life
of the activated carbon filters between two treatments v/as
longer than that of sand filters. We were also able to verify,
as others have done before  us,  the  rapid disappearance of the
adsorptive properties of the  carbon, except in matters of
flavour. However, during these  tests in the full-scale plant,
                                    s
chlorine-oxidized water was passed  through the filters. It
was therefore interesting to  judge  the effect of a  carbon
filter on water oxidized by ozone used in pre-ozonization.

Consequently, the following general, scheme wad adopted in
the pilot tests  (see Fig. 5):    ....
           Pre-ozoni-
                 WAC
                ClONa ClOj
                             carbon
                               sand
                       resx-
                      . dual' O,,0,4ppm,CI,
                      MM ELL
                                       io+
                  . Chemical Flocculation
                 treatment              Post-
                              FILTRATION ozonization RESERVOI*
                       DECANTATION
Fig. 5
General scheme  of  the experimental lines

-------
                         -  181  -  '
-  In the control line (ref. No. 1) the treatment corres-
   ponds to that in the plant, whose characteristics are
   as follows:
1)  At the pre-treatment stage: chlorination at the break-
                               point with Javel water;
                                                         /
                               addition of chlorine dioxide
                               to eliminate phenols, man-
                               ganese, and certain flavoursi

                               coagulation by means of
                               poly-aluminium chloride.


2)  After the O.4 g/m  residual post-ozonization treatment:

                     ..-.-,     post -chlorination.


-  In the experimental line (ref. No, 2), the treatments

   differ from the control line as follows:


   pre-ozonization carried out with semi-industrial
   emulsifier-type equipment. The total contact time
   is about 2 min, but the characteristic feature of
   the treatment is the time of contact of a bubble
   of ozonized air with the water, which is about
   2O sec.

   Omission of the chlorine pre-treatment.

   Filtration through activated carbon instead of filtration
   through sand as used in the control line.


The purpose of the experimental scheme for this line was to
favour bacterial development: oxygenation of the raw water,
omission of the chlorine pre-treatment, and suitable filtration

material. In the rest of this report, this will be known as

the biological scheme.


The tests were conducted with a view to determining the
effects of the pre-ozonizatiori conditions on the quality

of the water at different stages of treatment. Consequently,
the following operations were carried out during the essen-
tial phases of the tests:

-------
                         - 182 -


 1.  Operation for several months with constant pre-
                                                      3
    ozonization conditions: treatment dose of O.25 g/m
    and an air concentration  of 1.5 g of ozone per m  ,
    or an air/water ratio of  17 %.

 2.  Operation with pre-ozonization treatment dose vary—
                               3
    ing between O.2O and 4 g/m , bi
    air/water ratio of about  16 %.
                          3
ing between O.2O and 4 g/m , but with a constant
3.  Variation of the two characteristics of pre-ozonization,
    i.e. the treatment dose and the air/water ratio. This
    phase of the trials is currently in progress, and we
    can only give the initial results.
During all these experiments the treatments common to both
lines were applied under the same conditions. The treatment
dose of coagulant was determined by the jar test, and the
post-ozonization was chosen with a view to obtaining a resi-
dual virulicidal content of O.4 mg/1 after 1O min of contact.

We shall discuss the quality of the water tested at different
stages of treatment during the various phases outlined above.
EFFECT OF PRE-OZONIZATION ON RAW WATER
As regards the raw water, the results obtained over a period
of three months  (trial with pre-ozonization at a constant
strength of O.25 ppm) enable us to draw the following basic
conclusions (see Fig. 6).
The pre-ozonized water is richer in suspended matter, it is
more turbid and more clogging. This development is due to
the decomplexing and coagulating properties of ozone.

-------
                          - 183 -
Pre-ozonization



t
Before
After
Turbidity
(drops of
mastic)


1)-00
^30
Suspended
matter
(mg/1.)


5*
72
Beaudrey
clogging
power
U-1 )

\
7
9.4
N.V.T.O.C.
(mg/1)



7
7
UV absorp-
tion at
250 nm
(O.D.
. 10-3 cm"1)
1W
. 139
 Fig. 6    Quality of the raw water before and after
           pre-ozonization
As regards organic matter, the smaller value of UV absorp-
tion in the pre-ozonized line certainly confirms the action
of ozone on organic molecules - the total amount of organic
matter remains unchanged, as indicated by the values of non-
volatile organic carbon.

When the intensity of the pre-ozonization treatment is in-
creased, the difference in UV absorption between the two
treatment lines also increases, as long as the pre-ozonization
treatment dose does not exceed about 1 mg/1. Beyond this
threshold level the difference between the UV absorptions
remains essentially constant (see Fig. 7).

This result shows that pre-ozonization does not eliminate
organic matter but modifies their structure. This action
increases with increasing doses of ozone until about 1  mg/1
is reached.
The results obtained with an air/water ratio of 16 % for the
pre-ozonization seem to be fairly analogous regardless of the
air/water ratio, as shown by the first results of the most
recent tests still in progress.

-------
                          - 184  - •'
   w
 30
 20
 K>
        at 250 urn
                 o
               o    o
             125     2  Pre-ozonizatio
                       dose (rag
 Fig.  7
"Reduction  of  UV absorption
 as  a  function of the
 pre-ozonization dose
The amount of oxygen  dissolved  in  the water increases sig-
nificantly as a result of  pre-ozonization.  During the last
tests, carried out in August 1978, .pre-ozonization enabled
the amount of dissolved oxygen  to  be increased from a satur
ation level of 75 to  85 %  in relation to the atmosphere.
AMMONIA CONTENT IN THE TWO  TREATMENT LINES
Ozone has no direct effect  on'the  elimination of ammonia.
There is therefore no difference between the ammonia concen-
trations in pre-ozonized  raw water and initial raw water.
At the stage where  the water  is  decanted,  the experimental
results show that after  a  period of one month there is a
very marked reduction of the  ammonia level in the pre-
ozonized line. The  values  obtained are of  the same order as
those in the control line,  in-which the breakpoint pre-
treatment was kept  up during  the firs,t five months of the
tests (see Fig. 8).            .-.,-•

-------
                         - 185 -•
 NH.
   ppm
0.5.
                                      raw water
                                      decanted water 1
                                      decanted water 2
                                      filtered water 2
   x*i  B«c.ll   JM.JI   Feb.   March  April
 Pig.  8    Ammonia  contents
June    July
In the pre-ozonized  line  biological elimination took place
in the layer of sludge  formed in the flocculators,

During the subsequent filtration the same test -shows that the
ammonia remaining after decantation is easily eliminated. A
delay in inoculation was,  however,  necessary for the new
activated carbon introduced into the filter.
Nitrification seems  to  occur principally in the upper layer
of the filter, as evidenced by the counts of nitrite and
nitrate germs at different depths.

Prom the beginning of June 1978 the treatment at the break-
point was discontinued  in  the control line. It was ascertained
(see Pig. 8) that nitrification was just as effective in the
decantation unit without pre-ozonization. Under these con-
ditions, this treatment contributed  nothing to the bio-
logical elimination  of  ammonia.
For the amounts of ammonia of the order of 2 ppm that can be
encountered in the river,  .the dissolved oxygen will, however,
be insufficient. Pre-ozonization then will provide enough
oxygen to bring about nitrification.

-------
                          - 186 -


Thus, although pre-ozonization has no direct effect on  the
elimination of ammonia,  it does seem to be useful in this
respect for bringing about biological nitrification when
the amount of ammonia present exceeds a certain limit.
However, the essential advantage of replacing chlorine  by
ozone in the pre-oxidation is that no halogenated compounds
are formed during  the pre-treatment.

The content of chloroform in the treated water after the
final protective chlorination treatment was markedly smaller
in the biological  line (Fig. 9). This infers a significant
elimination of precursors by a biological route.


                                 A   x raw water
                                     treated water,
                                 ,.„__ control "*-* —
                                 __._ treated water,
                                     biological line
        IM. 77
                   Jit. 79
Feb.
                                       March
   Fig.  9  Chloroform content
ELIMINATION OF  ORGANIC MATTER FROM DECANTED WATER
The effect of pre-ozonization in the pre-treatment on
coagulation - flocculation - is very marked. The flocculate
in the treatment  line, where pre-ozonization had been used,
was far coarser,  as  can be seen from" Fig. 1O.

-------
                         -  187 -
 Flocculate in the                 Flocculate in the
 pre-ozonized line                 non-preozonized line
 Fig. 1O
The effects of the pre-ozonization can be noticed from a
dose of about 0.2 ppm onwards. Pre-ozonization is therefore
more effective as an oxidizing pre-treatment for coagulation
than is pre-chlorination. This advantage indicates an im-
proved elimination of the organic matter in the line with
pre-ozonization.

What is the situation with respect to the organic carbon
parameter? The results obtained with UV absorption of the
raw water have shown that it might be useful to proceed
to pre-ozonization with relatively hi'gh doses  (about 1 ppm) .
This supposition was confirmed for the decanted water, where
it had been found that the difference between the organic
carbon contents in the two treatment lines increases in
favour of the biological line, with an increasing pre-
ozonization dose of up to about O.6 mg/1 (cf. Fig. 11).
Above this dose the difference between the organic carbon
contents is O.7 ppm.

When the treatment at breakpoint in the control line is
discontinued, it seems that this difference is not altered
to any significant degree. The results obtained so far
indicate a mean difference of 0.6 ppm.

-------
                         - 188 -
 NVfOG
0,5
        0.6
                          Fre-ozonlzation
                          dose (ing Oyl)
 Fig.11  Seduction of  the  content  of  non-volatile organic
          carbon in decanted water  as  a  function of pre-
          ozonization dose
Thus, pre-ozonization  is certainly  the reason for the better
results obtained with  decanted water  in the biological line.
ELIMINATION OF ORGANIC MATTER  IN THE  FILTERED  WATER
The first measurements in  filtered water showed very low
contents of organic carbon in  the biological line.  This  was
in no way due to the biological activity of the filter,  but
to adsorption on the activated carbon, which had only just
been placed in the filter.
At the end of several months of operation at the rate of
4 volumes per hour the findings corroborated earlier ex-
perience and the results reported by  many other investi-
gators: the effects of adsorption are then very much reduced

-------
                          - 189 - V- T  •


and essentially constant. Any improvements observed  in
the filtered water are thus a result of  biological activity.

The measurements taken in the course of  the  test with vary-
ing pre-ozonization doses show differences between the  organic
carbon contents in .the two lines which are much the  same for
the filtered water and for the decanted water.

However, the results  obtained are certainly  not wholly  in-
contestable . Thus , the time after every  change in the pre-
ozonization conditions, about a week, was perhaps too short
to have allowed the bacterial population of  the filter  to
adapt itself to each  new situation.

In order to evaluate  the biological  activity of the  filter
with respect to organic matter, we also  measured the content
of detergents, compounds that are easily biodegradable. The
results obtained show that during filtration through activated
carbon this parameter was only reduced to about 7O % (cf.
Fig. 12) .
 100
 50
  Q
   Hit.  nc.77   Jn.78    Feb  jferfch   April   May    June   July'

Fig.  12     Reduction of the amount of detergent by
            filtration through carbon  (biological line)

-------
                         - 190 -
It is interesting to compare this finding with those obtained
in a test on filtration through carbon, carried out with
filtered and ozonized water produced in a large plant. It was
noted at the time that the amount of detergents was totally
and constantly reduced, even though the water had been pre-
treated at the breakpoint. However, this total elimination
was obtained only when the flow rate had been reduced to
12 m/hf which corresponded to 4 volumes per hour (see Fig. 13).
                                   V = l2.«/h	
            2    34    56     789    10   11
Fig. 13   Reduction of the amount of detergents by
          secondary filtration after post-ozonization
Subject to the speed of filtration through activated carbon
not being too great, these results lead us to the conclusion
that far more efficient biological activity as regards
organic matter can be obtained on a filter after secondary
ozonization  (ozonization after complete clarification) than
on a filter placed in first filtration, even preceded by
pre-ozonization. It is highly probable that the lower bio-
logical yield with respect to organic materials observed
with the latter type of filter is due to the periodical
washing to which the filter is subjected.

-------
                          - 19-1 .--'-
 We are therefore continuing with the pilot  tests,  studying
 the efficiency of filtration after  secondary  ozonization
 to complete the elimination of organic substances  already
 achieved in the biological line that we have  been  testing.
 The initial results of these trials have been fairly en-
 couraging.
 EFFECT OF PRE-OZONIZATION ON VIRULICIDAL POST-OZONIZATION
 Another very important action of pre-ozonization  is the
 effect that it has on the behaviour of water  during post-
 ozonization. This treatment was tried out  in  a  pilot line
 with a view to obtaining a residual virulicidal content
 of 0.4 g/m  in the water for a time of 10  min.
 It was found that, in order to arrive at  this  result,  the
 treatment doses required should be 40 % to  5O  %  lower  in
 the biological line than in the chemical  line. This  saving
 on ozone in post-treatment remains fairly constant for all
 pre-ozonizations above 0.25 ppm  (cf. Fig. 14).
  70

  60.

  50.

  4O

  3O

  20

  10.
4.Reduction of
 post-ozonization
 rdose  %
                                           Pre-ozonization
                                                dose,
              0.5
Fig. 14   Reduction of the post-ozonization  treatment dose
          as a function of the pre-ozonization  dose

-------
                         - 192 -
Since the mean post-ozonizatidn dose in the treatment of
Seine water is 2-. 5 ppm, the application of pre-ozonization
at 1 ppm will not necessitate the installation of supple-
mentary ozone-generation equipment. All that will be re-
quired for pre-ozonization is a different distribution of
the existing production. Thus, pre-ozonization can be
considered to be "gratis".
BEHAVIOUR OP TREATED WATER IN THE DISTRIBUTION NETWORK
To compete the appraisal of the treatment lines tested, the
pilot line was equipped with two mini-networks made of un-
lined cast-iron pipes 10O mm in diameter. The water moved
very slowly in these pipes, to obtain a residence time of
4 days (see Fig. 15). Sampling points were provided to allow
the water to be collected after residence times of 1, 2,
3 and 4 days.
                                Fig.  15
                                Mini-network  in
                                the  pilot  plant
The counts of the banal bacteria present in these samples
enabled the risk of bacterial re-growth in the water
produced to be calculated. The figures obtained for the
water produced in the tests show that in the biological

-------
                          - 193 .-
line the maximum count reached was about  2OO  bacteria/ml,
and in the control line only 15O/ml. This difference is of
little significance, and it can be assumed that bacterial
re-growth will be identical for the two types of water
(cf. Fig. 16).
    tN/ml
  to1
  10'-
 eontrol
.* biological
                             Days
             Fig.  16

             Banal bacteria  counts
             in  the mini-network
CONCLUSION
The results of  the  tests  indicate that the pre-ozonization
treatment of  Seine  water  before coagulation presents many
advantages over the chlorine pre-oxidation practised so far.
-  It enables the pre-chlorination treatment at the breakpoint
   to be discontinued,  for it constitutes the first link of a
   chain in which ammonia is eliminated in a biological way.

-------
                         -  194  -
This solution to the problem entails a reduction of the amount
of organochlorine compounds in the'water produced, and in
particular of haloforms, this being due to the elimination
of precursors in the course of the biological clarification
process.

—  Pre-ozonization leads to an appreciable improvement in
   the elimination of organic matter, essentially in the
   decantation units.

It does not appear, however, that this improved elimination
occurs by the biological route during filtration through
activated carbon.
The use of a second filtration after secondary ozonization
is undoubtedly necessary to obtain a complementary increase
of elimination of organic substances by a biological route.

-  Lastly, it must be pointed out that pre-ozonization does
   not entail any supplementary use of ozone, bearing in
   mind the economies that such treatment would bring to
   post-ozonization.

-------
                          —  195 -  '


PHYSICAL-CHEMICAL PRETREATMENT FOR THE REMOVAL
OP PRECURSORS

J.M. Symons and A.A. Stevens


INTRODUCTION

The reaction of chlorine and precursors to yield
chlorinated organics, as represented by Figure 1,
would indicate three possible ways exist to attack the
trihalomethane problem:  rj.) is the removal of the  pre-
cursors by some treatment technique;   2) is to replace
chlorine with some alternate disinfectant, or 3} is to
remove the chlorinated organics once they are formed.
This paper will review what is' known about removal of '
precursor as a trihalomethane control procedure.
Discussion of the other two techniques can be found
elsewhere (1,2,3).
CHLORINE +  PRECURSORS
 Fig. 1
CHLORINATED
 ORGANICS

-------
                           - 196  -
 REMOVAL OF PRECURSORS


 For removal of precursors,  three .possible techniques
 have been explored: precipitation, oxidation,  and ad-
 sorption, precipitation  being either during lime  soften-
 ing or during turbidity  and color, removal with a  co-
 agulant, oxidation either "with ozone, chlorine dioxide,
 or potassium permanganate,  and adsorption either  with
 powdered or granular activated carbon.


 Precipitation


 Pilot plant data would indicate that the amount of
 chloroform that is formed during treatment can be
.changed depending on the point of application  of  the
 chlorine (see Figure 2),  either ahead of the flocculation
 basin, ahead of the filter, or after filtration.  This
         1 25
RELATIVE
TERMINAL
CHLOROFORM 10
CONCENTRATION
IN FINISHED
WATER AFTFR 75
2 DAYS AT
GIVEN POINT OF
CHLORINATION  s
COMPARED TO
CHLOROFORM
FORMATION
POTENTIAL IN
RAW WATFR
  RIVER
25
                RAW WATER TERM

                  CHCI3 CONC
 FERRIC
}SULFATE
(COAGULANT
                  E
                   ALUMINUM
                   SULFATG |
                   COAGULANT
t
       2 DAYS RAW
       vWATER
        ..STORAGE
O>
                          © .
                          "
                                DISTRIBUTION
                                 SYSTEM
 COAGULATION/" FILTRATION
 FLOCCULATION.
 SETTLING
Fig. 2  Chloroform in finished water  relative to
        point of chlorination  (pilot  plant studies)

-------
                          -  197- -


indicates that precursors are being * removed'during treat-
ment, and the later in treatment that chlorine is
applied, the less chloroform is produced. In this par-
ticular water from the Ohio-River, ferric sulfate was
a more effective coagulant and.sb.the solid part of the
bars in Figure 2 indicates the  ferric sulfate data,
the total bar the aluminium sulfate data.
Raw water data are also indicated in Figure 2 for the
terminal trihalomethane concentration and a question
frequently arises as to why the raw water terminal
trihalomethane concentration is higher than the amount
of trihalomethane produced when raw water is chlorinated
in a treatment plant. The explan'atioi is that determi-
nation of terminal trihaIbmethanes is a test that is
done in a bottle in which raw water and chlorine are
mixed and held for some time and the resultant trihalo-
methane measured. In the 'treatment plant the chlorine
is added, and as the water is passing through the co-
agulation and sedimentation basin the precursor and the
chlorine are being separated by the settling process.
Less trihalomethane is produced under these conditions
than would be produced if the raw water was chlorinated
in a bottle with precursors and chlorine in contact
for the'duration of the holding period. Therefore, the
raw water terminal trihalomethane is used to indicate
the potential in the water, but this potential is not
realized even when the"raw water is chlorinated during
treatment.
Precursor removal by coagulation and settling was first
(1975) demonstrated on a full ;scale at Cincinnati,Ohio,
U.S.A. Figure 3 is a diagram of 'the Cincinnati Water
Works. Formerly they added chlorine at point A, where
chlorine and coagulant were both added. Then they moved
the point of chlorination from point A to point B, the
head end of the treatment plant. Because alum was added
before presettling  (point A); "some particulate removal

-------
                          - 198 -
occurred in these b'asins so the water  quality  at point
B is considerably better than  it would be  at point A.
The results of this change of  chlorination practice
are shown in Figure 4. After the point of  application
of chlorine was moved from point A  to  point B,  the
chloroform level dropped very  dramatically.
In order to demonstrate that this was  really caused by
the moving of the point of application of  the  chlorine,
and not because of a change in the  river quality, the
raw water trihalomethane formation  potential was
measured at various times throughout this  period to
show that precursors still did remain  in the raw water.
Even though a slight downward  trend in concentration
may have occurred through the  winter months (Figure 4),
it was certainly not equivalent to  the dramatic drop
in chloroform concentration that occurred  when the
point of chlorination was moved. Note  that the bromin-
ated compounds did not change  nearly as much in con-
centration on a percentage basis as did the chloroform.
                       OFF-STREAM STORAGE RESERVOIRS
                                       3 DAYS)
   POINT B
  WATER
TREATMENT-^
  PLANT
FILTERS
                    POINT C
              CLEAR WELL
        (TTt
    DISTRIBUTION SYSTEM
CHLORINE, ALUM-1  PUMPING
    POINT A  ( ^STATION-
                                                 \ INTAKE
  Fig. 3  Schematic of Cincinnati Waterworks

-------
                          -'199  -.
     300r
        MOVE CHLORINATION
     2600 FROM POINT "A"
        TO POINT "B"
       -JULY 14, 1975
     220
     180 -
TRIHALO
METHANE
CONC.
     14O -


     100 -


      60 -


      20

      NF
      A

-CHLOROFORM'
    A
               KEY
        RAW WATER CHLORINATED
        AND STORED:
          a 3 DAY TERMINAL CHLO
         RQFORM CONCENTRATION
          ©4 DAY
        '         :      .  •
          A 6 DAY
-BROM ODICH LOROM ETH A NE
,DIBROMOCHLOROMETHANE

                                 Fig. '4 '
                                 Trihalomethanes in
                                 Cincinnati, Ohio
Another  trial was conducted in the  late  summer of 1977.
In  this  case, the point of application of chlorine was
moved  from the rapid mix  (hydraulic jump)  to a point
following sedimentation and before  the rapid sand
filters.  Table I shows the trihalomethane concentration
in  the distributed water  for  1976  during mid-September
and early October and contrasts  these data to those
collected during the 1977 study. The generally lower
concentrations during the 9/28/77-1O/7/77 test again
show the advantages of chlorinating as high a quality
water  as possible.
Figure 5 is a schematic diagram  of  the Daytona Beach,
Florida,  U.S.A., treatment plant. This utility did a
study  in which -they chlorinated  at  three different'
points,  1)  chlorinating the raw  water, 2)  adding chlorine
at  the recarbonation basin, and  3)  adding chlorine at the
.clearwell.  All of the trihalomethane concentrations were
measured at sample point No.  5,  which was several hours
flow time following the clearwell.

-------
                         - 2OO X'.
TABLE I  INFLUENCE OP MOVING POINT  OF  APPLICATION OF
         CHLORINE AT THE CINCINNATI WATERWORKS
Control Year
Point of Application
Bate of Chlorine

9/16/76 Hapid Mix
21
23 "
28 "
29 "
3O




10/5

7 "


12
14
ZTHM in
Distributed
Water
Jif/1

12O.1
117.5
117.8
119.7
108.9 . .
115.8 •




109.7

114.O


92.1
109.0
Experimental Year
Point of Application
Date of Qilorine
9/13/77 Eapid Mix

21 "

28 Settled Water
. , .29- .'
30
,;10/1
2 "
3 "
4
. . .5 - "
6
. • 7
1O ; Rapid Mix
•11


EIHM in
Distributed
Water
jig/I.
no. a

107.4

81.5
1O2.4
76.0
89.2
87.4
116. 0
78.1
77.7
77.7
81.0
112.9
89.9



-------
                          - 201 - ,
                                       CUMWtU.
Fig. 5  Flow diagram Ralph  F.  Brennan Water Plant,
        Daytona Beach, Florida
3UU
450

400

350
300
iTHM,
U9/l 250

200


150
100
75
50
25
ft
DAYTONA BEACH, FLA.
T


1




1


ti2
RAM
NO
C02



^
'/////////////A








i
L }

f
co;












W/////////A
CI2
SETTLE
2 NO
CO2









^
^
§>
"T
TERM
, IN RAW "
WATER
i
i


D El INST iTHM
f—i^THM FORM,
L— J POTENTIAL
G3«QTERM STHM
CO2 CI2





W/////////A
FILTtRtD
NOCO2
CO2

^ ^
^ ^
$0 V^
^ V
                                      Fig. 6

-------
                           -  2O2  -  .   .


The first two bars in' Figure 6 are weekly average data
resulting from chlorinating the  raw water; the second pair
from chlorination of the settled water; and the third pair
from chlorination of the filtered water. The cross-hatched
section of the bars shows  the instantaneous values that
were measured at sample point 5  and the open section addi-
tional trihalomethane formation  potential that would be
exerted out in the distribution  system.

The series of studies were conducted both with and without
recarbonation, and recarbonation had some effect on the
instantaneous trihalomethane concentration because of
stripping. In summary, Figure 6  shows two important points:
one, the raw water has essentially the same formation poten-
tial or precursor concentration  throughout the study and,
secondly, the benefits of moving the point of application of
chlorine are shown. The removal of precursor through coagu-
lation and sedimentation was again demonstrated. The project
is continuing in Daytona Beach attempting to demonstrate
whether or not this effect can be improved even further
through the use of the addition of polyelectrolytes.


Oxidation
   Ozone
Table II shows the effect of ozonation as an oxidant on
pilot plant filtered water in Cincinnati, Ohio, U.S.A.
Ozone alone does not produce any trihalomethanes.
For the first study without ozone and with chlorine,
2O yg/L of trihalomethane was produced. With O.7 mg/L
of ozone plus chlorine,  23 yg/L of trihalomethane was
produced. The effect of ozonation was negligible. The
same occurred with a dose of 18.6 mg/L of ozone.  When
a very high dose of ozone was used, 227 mg/L,  some
oxidation of precursor occurred.  Therefore,  ozone can
be effective in certain circumstances,  but in the

-------
                          - 2O3 -
Ohio River water it certainly was not particularly
effective for precursor oxidation at this time.
Table II  EFFECT OF OZONATION OF DUAL MEDIA EFFLUENT
                   Contact Time - 5-6 min.
Applied 0.3
Dose
mg/L
0.7
O
0.7
18.6
O
18.6
O
227
Chlorine
Dose
mg/L
O
8
8
O
8
8
8
8
animation Trihalctnethane
Cone, after 6 days
yg/t-
< O.2
2O
23
< O.2
23
30
123
70








Figure 7 presents the results of another study per-
formed with Ohio River water during a different time
period showing that in this case a big decline in
precursor occurred. This study also included an
evaluation of the effects of ultraviolet radiation
which further enhanced the effects of ozone on re-
moval of precursors. The test system was a 22 liter
batch reactor receiving 2O mg of applied ozone per

-------
                        .. -,-204 -
minute. Although this study was also conducted on
Ohio River water, the results were quite different
from those shown in Table I.

The variability in the observed effect of ozone on
trihalomethane precursor removed is further shown
by Table III as compiled by Trussel. (4) These data
from the independent work of several investigators
range from negative removal to 9O percent. Several
variables undoubtedly account for the observed wide
range of results: nature of precursor, ozone dose,
contact time, contactor design, including the dis-
persion system, and possibly other water quality
factors.









7-DAY
TRIHALO-
M ETHANE
FORM,
POTENTIAL
(um/1)










2.10
2.00

1.90
1.80
1.70
1.60
1.50
1.40
1.30
1.20
1.10
1.00
.90
.80
.70
.60
.50
.40
3O
»»JW
.20
.10
0

t
I
\
A
!\
d. _^__ __ ~_
all— -*: ^~~-»_OXYGEN ONLY
lj\ / \ .---*x~~~*
\ V %*'-^..-*""" *\
J'^ \UV ONLY
P1
i\\
ll
'^\
* \T*^
\ '^">».
&\ ****
\ Sm--~, OZONE ONLY
\. ""*"*
^V"*
\v.
Tfc:-s='-'~:t" 	 • OZONE
""*=« PLUS UV
0 15 30 60 90 120
TIME (WIN.;
 Fig.  7  Ozone and ozone-UV destruction of THM precursors
         Ohio river water (2/77)

-------
                        -  2O5 -
Table III  PRECURSOR REDUCTION WITH OZONE
Location
Owens River
Lake Casitas
Columbia River
ii
n
ii
Ohio River
(Louisville)
n
ii
M
Bay Bull's Big Pond


Ohio River
( Cincinnati)

. Mokelumne



Middle River




Rotterdam

Orange County
Caddo Lake, Texas
Ohio River
Dose, mg/L
1 .0
2.0
0.5
1.0
2.0
4.0
1 .O
2.0
4.0
6.O
8.0
1 .0
2.0
3.O

18.6
227
2.0
3.4
4.5
6.O
2.6
2.8
5.5
10
11
2
8
1.0
O-72
O- 109
% THM Precursor
Reduction
78
6
8
14
16
16
6
22
3O
46
46
13
19
27

-31
43
62
59
59
53
-13
- 3
32
: ' 7
22
6O
50
7
O-9O
O-8O
    from R. Trussel,  1978  (4)

-------
                          -  206  -
-  Chlorine  Dioxide

The data  presented in Figure  8  show some removal of
precursor by application of chlorine dioxide. Th**
upper curve  is  the trihalomethane  formation with
chlorine  alone,  the middle curve is similar data
with chlorine dioxide and the same amount of chlo-
rine. The chlorine dioxide has  some effect on the
precursor such  that it is changed  so that it will
not react with  the chlorine to  form trihalomethanes.
The lower curve shows that chlorine dioxide alone
does not  produce trihalomethanes.
I 0.6
3
Z0.5

lo.4
JE 0,1
F.A. Cl = FREE AVAILABLE CHLORINE
pH = 7.4
T = 24°C
O 0.3      «-~ - m9/' F-A'CI
O
O
         mg/l CIO2 + 1-5 mg/l F.A. CI
             ClOa ALONE
UJ

<   O   10  20  30  40  SO 60  70  80
35          CONTACT TIME, HOURS
DC
 Fig.  8  Trihalomethane  formation by C1O2 and excess  free
         available chlorine,  ERG Pilot Plant settled
         water  (6}

-------
                           - 207 -

   Potassium Permanganate

Table IV presents the results of some preliminary
studies investigating the effects of potassium
permanganate on natural precursors from the Ohio
River. In these experiments, the percent removals
of precursor are relatively small, the highest is
19 percent, the lowest is 3 percent. Thus, potassium
permanganate does not have large influence on the
precursors that are in the Ohio River, although it
does do some good. As mentioned in a previous paper
(5), permanganate may have a more dramatic effect
on precursors in some waters where precursors were
observed to react more like m-dihydroxy phenyl
moieties.
Table IV  PRELIMINARY DATA ON PRECURSOR REMOVAL FROM
          OHIO RIVER WATER BY POTASSIUM PERMANGANATE
KMnO. Reaction
Amount
Added
(mg/L)
O
5
0
5
0
5
0
5
Reaction
Time
(hours)
1.5
1.5
1.5
1.5
1.5
1.5
0.5
0.5
pH


7.1
7.1
9.3
9.3
10.2
10.2
Neutral 7.O
Neutral 7.0

Chlorine
Reaction
Time
(hours)
2
2
2
2
2
2
30
30




ZTHM




% Removal

pg/L
30.
25.
52.
50.
57.
54.
120.
97.
5
7
3
9
2
0
5
6

15.4

2.7

5.6

19.0

-------
                           - 208 •-
Adsorption

-  Powdered Activated Carbon

Figure 9 presents some  typical  data on the performance
of powdered activated carbon  (PAC)  for precursor
removal. In this experiment chloroform formation
potential declined  some initially,  and then decreased
more slowly as a 100 mg/L  dose  was  approached. These
data would tend to  indicate that a  5 or 1O mg/L PAC
dose would give an  effect  that  might be favorable if
a finished water had a  trihalomethane concentration
just above the proposed regulation, but PAC could not
completely eliminate precursor  even with an uneconomical-
ly high dose.
 o>
 -i 30
 P
 I 25
 O
 Q.

 I 20
 O
 u.
 5 1(=
 CE 1£>
 O
 u.
 O
 §10
 2
 O
 O 5
 Q
 in
2 MINUTES RAPID MIX
5 MINUTES SLOW MIX
30 MINUTES SETTLING

 DO    10    20    30    40    50    60    70    80    90   100
 N          POWDERED ACTIVATED CARBON DOSE, mg/l

 Fig. 9  Influence of powdered activated carbon on
         chloroform formation potential

-------
                            -  2O9 -

-  Granular Activated Carbon                  ,   ',.,.....•

Figure  1O  shows data from  a  treatment plant in
Huntington,  West Virginia, U.S.A. The precursor  is
nearly  completely removed  initially and then  the
concentration steadily rises in the adsorber  effluent,
reaching the proposed maximum contaminant level  (MCL)
after about four weeks. Notice that the empty bed
contact time here is only  6-1/2 minutes, which  is  a
short empty bed contact time.  Nevertheless, when the
granular activated carbon  is fresh, it will remove  pre-
cursor  well,  but then a slow steady break-through
occurs. Other data with deeper beds (longer empty bed
contact time)  tend to show that the time period  for
break-through to the proposed MCL is longer,  Figure 11.
Figure  12,  data frdm the granular activated carbon
treatment  of water with a  very high concentration of
trihalomethane precursor;again demonstrates the  in-
fluence of empty bed contact time.
SUMM,
TRIHALO- O.I SO
METHANE
FORM.
POTENT.
CONC.
me/I
O.14O
     0.120
         [SAND REPLACEMENT
         (ADSORBER"""   ,  PROPOSED [MCL|  \
        ""'NFLUENT    f      I
                SAND REPLACEMENT!
                ADSORBER EFFLUENT ;
        «PPROX, EMPTY BED CONTACT TIME = 6.5 MINUTES
        II 15 20 25 27291 3 5 B 10121517 1922 24 26 29 31
          JULY       AUGUST
                             Fig.  1O
                             Removal  of  trihalomethane
                             precursors  by granular
                             activated carbon beds

-------
                            - 210 -
  24 MiN. EBCT
         INFLUENT
              a
              PROPOSED MCL
                                             v. o
                                              b".
                -7" "
0    W   20   30   40   50   60   70   60   90   100   110   120  130  "0   1&Q   WO   -70  iflO
             Days ol run
Fig.  11   Performance of terminal THM through Post Filter
          Adsorber  run 1 (Feb.-Oct.1977)  Jefferson Parish, La^
0.80
0.70

0.60
<
0.50
TERM.
SUMM,
TRIHALO °-40
METHANE
ARROW INDICATES PROPOSED
• P MCL EXCEEDED 1
/\ / —INFLUENT j
/
/

! ' P
*
• 1

'* '* '
^ :
*-* " i
5   10   15  20   25   30
   TIME IN SERVICE. DAYS
                                    35
Fig.  12   Example  of  the influence of empty  bed contact
          on terminal summation  trihalomethane removal

-------
                           -  21 1
                   BToiogicaily "Active" 'Carbon  Fi Iters
A study on  the  utility of preceding granular activated
carbon by ozone treatment on the removal  of trihalo-,
methane precursor was conducted in a pilot plant
treating settled Ohio River water. The  data in Figure 13
show that preceding the adsorption system" by an ozone
dose of about  1 mg/L, contact time 2O minutes, did re-
sult in a reduction in trihalomethane precursor in the
adsorber effluent at any given service  time when
compared to conventional g'ranular activated carbon.
Both adsorption systems had a 9 to 1O minute empty
bed contact time.  The mechanism  for this improved
performance is  thought to be related to an enhance-
ment of biological activity in the activated carbon
adsorber brought on by the ozone pretreatment.
       0,1
      0,08
  --O.OS
  STHMFP
  CONC,
  ma/l  0.04
      0.02
         	1	1	1	r—	1	r
         VALUES COMPUTED FROM MONTHLY AVERAGES OF
         WEEKLY DETERMINATIONS 2 DAV, 25°C SUMMATION
         TRIHALOMETHANE FORMATION POTENTIAL
                      4567
                       TIME IN SERVICE. MONTHS
                                                   10
 Fig.  13  Influence of  ozonation prior to adsorption
          on trihalomethane  formation potential removal

-------
                          -  21 2  -


SUMMARY
Precipitation, oxidation, and.adsorption are all
methods of removing trihalomethane precursor. Of
these various techniques, only adsorption on fresh
granular activated carbon is completely effective,
although the capacity of the activated carbon is
finite. The other techniques, alum or iron coagula-
tion, oxidation with ozone, chlorine dioxide or
potassium permanganate, or adsorption with powdered
activated carbon, are only.partially effective but
may still have a role in- controlling trihalomethane
concentrations in some cicumstances.

-------
                          - 213  -
(1)   SYMONS,  J.M.                                  -'•"'•' '
     Interim  Treatment Guide for the Control of Chloroform
     and Other Trihalomethanes
     U.S. Environmental Protection Agency, Cincinnati, Ohio,
     48 pp. plus 4 Appendices, Unpublished (June 1976)

(2)   LOVE, O.T., Jr.,  CARSWELL,  J.K., MILTNER, R.J., SYMONS, J.M.
     Treatment for the Prevention or Removal of Trihalomethanes
     in Drinking Water
     J. AWWA  (in Press)

(3)   SYMONS,  J.M., CARSWELL, J.K., CLARK, R.M., DORSEY,  P.,
    'GELDREICH, E.E.,  HEFFERNAN, W.P., HOFF,  J.C., LOVE, O.T.,
     Jr., McCABE,  L.J.,  STEVENS, A.A.
  .   Ozone, Chlorine Dioxide, and Chloramines as Alternatives
     to Chlorine for Disinfection of Drinking Water, State-of-
     the-Art
     Summary  in Proceedings of -Second Conference on Water
     Chlorination: Environmental Impact and Health Effects,
     Gatlinburg, TN, Oct.  31 - Nov. 4 1977 (1978), 555-56O,
     Complete  version available from Director, Drinking Water
     Research Division,  U.S. Environmental Protection Agency,
     Cincinnati, OH, 45268

(4)   TRUSSELL, R.R.
     Factors  Influencing the Formation of Trihalomethanes
     O'ames M. Montgomery Consulting Engineers, Inc., Pasadena,
     CA,  presented at the California-Nevada Section AWWA
     Seminar  on Organics in Domestic Water Supplies, Palo Alto,
     CA,  April 12, 1978

(5)   STEVENS, A.A. et al.
     Formation of  Non-Polar Organochloro Compounds as By-
     products of Chlorination
     Presented at  Conference on  Oxidation Techniques in  Drinking
     Water Treatment,  Sept. 9-13,  1978, at Karlsruhe, Federal
     Republic of Germany

(6)   MILTNER, J.J.
     The  Effect of Chlorine Dioxide on Trihalomethanes in
     Drinking Water
     M.S. Thesis,  University of  Cincinnati (1976)

-------
                          - 214  -
UNWANTED BY-PRODUCTS OE CHLORINATION
B. Josefsson
It is a challenge for an analytical chemist to determine
the different organic compounds  (in the nanogram per
litre level) which are present in drinking water. For
practical reasons I think it is meaningless to analyse
all of them.
A hierarchic analytical system for the analysis of
chlorinated compounds should begin with the determina-
tion of elemental chlorine for rapid screening of many
samples. In this way it is possible to locate sources
of organic chlorine compounds to the raw water or
during water treatment processes. ;When this procedure
reveals a source, a more laborious analytical determina-
tion of specific compounds can be performed. Dr. W. Kuhn
showed in his lecture that elemental chlorine analyses
can be carried out with the DOC1 (dissolved organic
chlorine measured by pyrohydrolysis) method and DOC1N
(dissolved organic ch^orine-nonpolar, measured with
microcoulometry on a nonpolar solvent extract)  (1).
Other methods in this respect include non-destructive
neutron activation analysis (NNAA)  on elemental chlorine
and bromine (2). When NNAA techniques are used it is
possible to distinguish between chlorine and bromine,
which cannot be done by coulometric methods, for example.
Liquid-liquid extraction with a nonpolar solvent is
very practical for the determination of lipophilic
organic halogens which can easily bioaccumulate. By
using this method organohalogenated compounds can be
tested for possible persistence by treating the extract
with cone, sulphuric acid, UV light etc. Bioaccumula-
tion and persistence are important in view of chronic

-------
                        '  - 215 -

                                      '' -  ' 1 ,- ' ,) - V ; i ." > • 1,T:< , > •
or long-term effects, aspects which have not been dis-
cussed here.
In water treatment processes chlorine is widely  used;
howeve , chlorine dioxide  has  gained popularity  especially
when tcs e and odour problems  occur upon conventional
chlorinat.-i.on. Chlorine and chlorine dioxide have also •
been extensively used in pulp  bleaching processes and
much of the experience gained  from these processes  may.
be of great value for water chemists. Bleaching  of  pulp
to eliminate miscoloring substances, e.g. lignin,  are
to some extent similar to  oxidation of humic substances
in water with different chlorine  species. However,  there
is a pronounced difference in  the concentrations of •
organic materials in the two processes.
Recent studies on by-products  formed upon pulp bleach-.
ing with chlorine species  have revealed great  numbers
of chlorinated organic compounds  of both polar and
nonpolar character in effluent water. The total  amount
of chlorinated compounds exceeds  1 ppm, expressed as
chlorine in both polar and nonpolar solvent extracts  of
the effluent water  (3). Only about a tenth of  the total
amount of lipophilic chlorine  has been characterized,
e.g. chloro-cymenes, chlorinated  terpenes etc.  (3)  (4).
The specific non—volatile  compounds which cannot be
separated  gaschromatographically are still not•very
well studied. This deficiency  may be partly overcome  .by-
use of chlorine detectors  in liquid chromatography. The'
waste effluents from bleaching exhibit mutagenicity
with the Ames test  (5).Through the polar character.of
chlorophenols  (from lignin'breakdown)/ these substances
have been found accumulated 'in fishes caught near the
bleaching waste outlet  (6).
Chloroform concentrations  are  about a hundred times
higher in chlorine bleaching effluents,than in drinking
water (7).  Hypothetically,' some.of'the other chlorina-

-------
                          - 216 -
ted compounds found in pulp bleaching effluents may
also be present in drinking water at a two-or three-
order of magnitude lower concentration. If they are
present in drinking water,do they have a long-term
effect?
(1)  KUlIN,  W., SONTHEIMER, H., STIEGLITZ, L. , MAIER,  D.,
    KUR8,  R.
    Use of Ozone and Chlorine in Water Utilities  in
    the Federal Republic of Germany
    J.  AWWA TO (1978), 326-331

(2)  LUNBE, G. ,  GET11ER, J., JOSEFSSON, B.
    The Sum of Chlorinated and of Brominated Non-Polar
    Hydrocarbons in Water
    Bull.  Environ.  Contain. ' Toxicol. 13  (1975), 656-661

(3)  EKLUND, G., JOSEFSSON, B., BjGRSETH, A.
    Determination of Chlorinated and Brominated
    Lipophilic Compounds in Spent Bleach Liquors  from
    a Sulphite Pulp Mill. Glass Capillary Column  Gas-
    Chromatography - Mass Spectrometry - Computer
    Analysis and Identification
    J.  Chroraatogr.  1 SO- (1978), 161-168

(4)  LINDSTR5M,  K. ,  NORDIN/J.
    Identification of Some-neutral Chlorinated Organic
    Compounds in Spent Bleach Liquors
    Sv.  Papperstidn. 81   (1978)', 55

(5)  ANDER, P.,  ERIKSSON,  K-E. , KOL7iR, M-C. ,
    KRIKGSTAD,  K. ,  RANNUG, U.,- RAMEL, C.
    Studies on the Mutagenic Properties' of  Bleaching
    Effluents         '
    Sv.  Papperstidn. 80  .(1977)., . 454 •

(6)  LANDNER,  L. ,  LINDSTR6M,' K. ,'KARLSSON, M. ,
    NORDIN, J., SOREMSEN, L..
    Bioaccumulation in Fish of Chlorinated  Phenols
    from Kraft Pulp Mill Bleclchery Effluent
    Bull.  Environ, "contain. Toxicol. J_8  (1977), 663-673

(7)  EKLUND, G. , JOSEFSSON', B. "/ ROOS, C.
    Determination of Volatile Halogenated Hydrocarbons
    in Tap Water, Seawater and Industrial Effluents  by
    Glass  Capillary Gas "Chromato'graphy and  Electron
    Capture Detection
    J.  High Resol.Chrom.  J[ (1978), 34-4O

-------
                          -  217  -
OCCURRENCE OF VOLATILE ORGANOHALOGEN COMPOUNDS IN THE OPER-
ATION OF WATERWORKS WITH VARIOUS TYPES OF WATER AND AMOUNTS
OF CHLORINE                •     •.
S. Hermann

Since the occurrence of low-boiling organic halogen compounds
in drinking water became known their formation in the water-
works as a function of the operating conditions has been of
particular interest.  Detailed investigations on this problem
were also started at. the municipal works in Wiesbaden.  The
following contains a brief report on the most important
results.               •  •

Use of chlorine' in the Wiesbaden water supply

In Wiesbaden chlorine is added at several points:

1)   In Wl-Schierstein Rhine water is treated by flocculation,
filtration, and activated carbon filtration and then by
seepage for the purpose of artificial ground water enrichment.
The first measure is to chlorinate the raw water from the
Rhine for the purpose of oxidizing ammonia and for disinfection.
The amount of chlorine added : is'such that after a. few minutes
0.5 mg/1 of free chlorine is still detectable.
2)   The drinking water obtained from the artificially en-
riched ground water - containing a considerable proportion of
bank filtrate from the Main'  —  after a further treatment by
aeration and slow sand filtration, is chlorinated to maintain
hygienic safety to the extent that it leaves the works with
0.4 to 0.5 mg/1 of free chlorine.  PAC filtration will be added
to the treatment.          -.  '
3)   A ground water charged with a small amount of anthropo-
genic material from the.Hessisches Ried, transported over
50 km to the supply area of Wiesbaden, must be chlorinated
several times on the way so  that bacteriological safety is

-------
                          - 218 -
ensured for the various smaller consumer communities situated
along the transport route, and on the other hand so that the
limiting chlorine level is not exceeded at any point.


Halomethane formation in the chlorination of raw water and
drinking water

It is worthwhile to compare the action of raw water chlori-
ration and drinking water chlorination in Schierstein as
regards the formation of organic chlorine compounds, and
also to compare the two drinking waters that come from very
different sources.

The three waters were studied gas-chromatographically for at
least 6 months for the content of organohalogen compounds
(after enrichment by pentane extraction and using an electron-
capture detector) and the low-boiling fractions were determined
quantitatively.  In addition to this, the DOC and the UV
absorption at 254 run were measured as sum parameters for the
organic loading.  The mean values from about 20 samples for
halomethanes, the sum parameters, and the amounts of chlorine
added are listed in Table 1.

As regards the concentrations of halomethanes, it is note-
worthy that these may be affected by an error resulting from
the unreliability of the determination of the partition co-
efficients.  With an enrichment factor of 200, a recovery
rate of 10% for chloroform and 33% for the other halomethanes
was calculated.

The interpretation of the results shall  thus be restricted
to the relationship of the individual values to one another.
Some interesting statements can then be made:

-------
Table 1   Mean values for halomethane formation in Wiesbaden waters
Halomethane concentration
in pg/1
Chloroform
Carbon tetrachloride
Bromodichloromethane
Dibromochloromethane
Bromoform
Chlorine addition, g/m^
DOC, .g/m3
UV absorption at 254 nm, m
Rhine water
Raw water
not
chlorinated
1.0
0.3
0.06
not
detected
rare
chlor-
inated
3.0
1 .2
. 4.5
1.2
n. detect.
...1.0
-5.5
4.5
9.8
Drinking water I
not
chlorinated
2.5
0.15
1 .2
0.15
not
detected
chlor-
inated
2.5
0.15
5.0
10. 0
5.0
0.75
1 .8
3.3
Ground water from '
Hessisches Ried .
Drinking water II -
Tl
chlorinated
4.0
0.1
4.5
0.3 -
not detected, , .0. 1
1.4
2.0
3.6
                                                                                     I
                                                                                     to

-------
                            22O -
1.   The haloraethane formation during the chlorination of raw
     water from the Rhine is only of the same order of mag-
     nitude as that during the chlorination of drinking
     water.  This is surprising, because the organic loading
     and also the chlorine consumption in the raw water is
     much greater than those in drinking water.  It must
     therefore be assumed that Rhine water contains relatively
     few halomethane precursors.

2.   Each compound shows different behaviour in different
     waters,

3.   The relatively sharp increase in the bromine compounds
     is striking in all three waters.

4.   Comparison of the two drinking waters clearly shows
     that even in the safety chlorination of a"natural,
     organically weakly loaded ground water halomethanes are
     formed in low concentrations.  In respect of the chlorine
     compounds listed the two drinking waters show approxi-
     mately the same loading.  The formation of dibromo-
     chloromethane and bromoform in drinking water I can be
     explained by the higher bromide contents in the enriched
     Schierstein ground water.

Formation of organohalogen compounds in chlorination of raw
water with variation of the amounts of chlorine added.

The effect of various chlorine additions and of various
excesses of chlorine in the chlorination of raw water was
investigated in a series of operational trials at the Rhine
water treatment works lasting several weeks.  The chlorine
was added directly before the addition of the flocculation
agent.  The effect of the chlorination was studied after
flocculation and filtration and a mean action time of 8 h.

-------
Table 2  Quality parameters in the four chlorination  trials
         (mean values)
Experimental
period
12.i2.77 to
11 .01.78
17.01.78 to
18.02.78
21.02.78 to
11 .03.78
21.03.78 to
25.04.78
Mean chlorine
addition
g/m
8.0
6,5
4,0
0
Chlorine concen-
tration in f loecu-
lation inflow _
q/mJ
1.1
0.5
0,1
0
Water
temp.
°c
4.4
3.8
5,5
9.6
pH
6.97
7.11
7.20
7.44
NH4+
rag/1
0.62
0.76
O.45
0.11
DOC
mg/1
4.9
5.0
4.8
4.2
CSB
KMn04
rag/1
2U7
21.1
20.3
17.3
OT254
m"1
10.7
10.4
10.1
9.1
                                                                                             to
                                                                                             to

-------
                           - 222 —
In Table  2  are listed the quality parameters, of the raw
waters  as mean values for the  experimental period for  the
four different additions of chlorine in the range between 0
and 8 g/m .   The lower concentration of free chlorine  in the
flocculation inflow shows that the bulk of the chlorine is
consumed  after only a few minutes.   Not until a dose of
8 g/m   can  appreciable residual concentrations of free
chlorine  be  measured, which act further during the subsequent
treatment.

The gas-chromatographic measurements were performed at three
temperatures,  namely at 60°, 180°,  and 230°C.  Fig. 1  shows
 O.Sn
6   8
       2468
y                ,
  Chlorine addition, q/ni —
      CHCIj
g 1.0'
o
Jos-
B
O
c.
6 n\

r
/ C2^
	 A 	 -/

                      HQ3
                      10
                     C2Ci4
       10
                                                     CHCl2Br
                                                      CHClBr,
        Mean values — Maximum values
                 8   10
Chlorine addition, g/ir,J

       Mean value* --- Maximum values
Fig. 1   Formation of low-boiling organohalogen compounds
         in  the  chlorinat.ion of raw  water in dependence on
         the amount of chlorine added.

-------
                          - 223
the concentration:increase for'6 different readily volatile
organohalogen compounds with increasing doses of chlorine.
In addition to the mean values, the maximum concentrations
found are also indicated, since they exhibit trends still
more clearly.

The concentration data refer here to values measured in
pentane, recalculated to the amount of water used but not yet
taking into consideration the partition coefficients for the
pentane-water system.

The different courses of the curves show clearly that the
formation of the individual compounds is strongly dependent on
the amount of chlorine:  while some compounds are only formed
with larger chlorine amounts, or when the chlorine is in
excess (e.g. trichloroethylene), in the majority of compounds
elevated concentrations can be detected after only small
chlorine additions.  The strongest dependence is given for
bromodichloromethane.  For the remaining compounds appreciable
concentration increases are only measured when the chlorine
addition exceeds 6 g/m , i.e. in
free chlorine is already present.
addition exceeds 6 g/m ,  i.e. in the range where an excess of
Most of the substances formed during chlorination are already
present in low concentrations in Rhine water.  Only very few
new compounds are formed, and these only at high doses. These
are low-boiling substances.  In addition to dichlorobromo-
methane,. seven new compounds were detected at a column temper-
ature of 60°C;  at 180°C only two were found in low concen-
trations.

As regards the higher-boiling substances (column temperature
180°C), the concentrations of the first four were increased by
the addition of chlorine  -  these were probably dichlorobenzene,
hexachloroethane, trichlorobenzene, and hexachlorobutadiene.

-------
                          -  224'"-
The concentration of the higher-boiling compounds is some-
times decreased by the flocculation  (by up to 50%).  Even
with high chlorine additions an increase was never observed.

An important factor for the evaluation of the effects of the
concentration increase of organic halogen compounds is the
ease of their removal during the subsequent activated carbon
filtration.

This was also studied, and in addition longer operational
experience is already available in this case.  The types of
carbon used (F 300 and P 400) are very effective as regards
the elimination of the DOC and the removal of high-boiling
nonpolar chlorine compounds.  The highly-volatile halogen
compounds formed mainly by the chlorination, however, are
only partially.adsorbed even by fresh carbon (e.g. chloroform
and carbon tetrachloride), or pass through the filter already
after a loading of only 10 1/g carbon (e.g. bromodichloro-
methane and dibromochloromethane).  With increasing loading
of the carbon these compounds can be found in the filtrate
long after they can no longer be detected in the raw water.
The high-boiling compounds (starting with bromoform) occur
only sporadically in the filtrate, in low concentrations.

Summary
The operational trials on the chlorination of raw water with
various amounts of chlorine, performed on moderately polluted
Rhine water (with average outflow), show clearly as regards
the gas-chromatographic substances that low-boiling organo-
halogen compounds are formed first.  The dependence on the
chlorine dose is very different for different compounds.
With low-dosage chlorination of the raw water with doses
           o
below 6 g/m   -  as in the Rhine water treatment works at
Schierstein —  the concentrations of readily volatile organo-
halogen compounds formed are kept within permissible limits.

-------
                          - 225 -
They are approximately in the same range as in the chlorin-
ation of drinking water.

If up to now chloroform formed the centre of interest, in the
 f
case of the chlorination of bromide-containing waters the
bromine-containing halogen compounds must receive'more
attention than previously, because these compounds too are
insufficiently eliminated by the activated carbon filtration
and form in appreciable concentrations even under the con-
ditions of low-dosage chlorination of drinking water.  In
this connection the bromide content of the- water also gains
in importance.

On the example of a marsh water (Hessisches Ried) it was
demonstrated that the safety chlorination necessary when
water is transported over a long distance - requires relatively
high doses of chlorine, and so can give rise to appreciable
halomethane concentrations.

-------
                          - 226 -


THM FORMATION IN TWO DIFFERENT WATER TREATMENT SYSTEMS
AT ROTTERDAM

J.J. Rook
1) Rotterdam Waterworks owns two treatment plants, i.e.
the Berenplaat Plant, dating from 1966, with a production
capacity of 12.0OO m /h, and the new Kralingen Plant, ca-
pacity 5.OOO m /h, which started production in July 1977.
Both plants treat the same type of raw water, which is
stored river Maas water. In the Berenplaat Plant conven-
tional chemical treatment is applied; breakpoint chlori-
nation, dosage of PAC, coagulation with Fe (III), sedi-
mentation, rapid filtration, post chlorination.

The new Kralingen,Plant consists of primary coagulation,
floe removal by means of lamella separators,  ozonation,
dual media filtration and filtration through granular
activated carbon, post chlorination.

The raw water has been stabilized by self-purification.
Algae control measures have proved to be very effective,
the average chlorophyll content being 5 ppb,  with inciden-
tal peaks of 4O ppb. The TOG value averages 5,5 ppm, mainly
caused by natural yellow acids which are identical with
fulvic acids. The raw water is transported through  28 -
3O km pipelines. During the summer period of 1977 the raw
water was pre-chlorinated (dosage circa 5 ppm)  before
transport in order to keep pipe walls clean from bacterial
growth. During the winter season no chlorination was app-
lied. Transportation to Berenplaat takes 6 h, to Kralingen
15 h. With the given amount of fulvic acids the pre-chlori-
nation resulted in THM formation:

-------
                          - 227-
      Plant .'"  ' -V Contact time  Temp.
      Berenplaat      6 h
      Kralingen      15 h
12-22°C
12-22°C
TTHM at
plant intake
   68 ppb
  1O3 ppb
During the winter season of 1977-78 pre-chlorination was
interrupted. Consequently, no~ THM formation occurred.
Results of conventional treatment

Table I summarizes the THM contents in the finished water of
the conventional treatment at Berenplaat, both with and without
pre-chlorination.
TABLE I  THM in Berenplaat Plant
         Conventional: Breakpoint-PAC-coag.-filtr.



o
o
CM
CM
1
CM
t —



O
o
CM
3 —
I


Raw water
pre -chlorinated
(5 ppm 7 h)

Finished water
Raw water

not chlorinated

Finished water
(4 ppm breakpt .chlor . )
CHC1-
3

30 '


40




18

CHCl_Br
2

22


28

O


13

CHClBr,,
2

14


14.5

0


6

CHBr_
3

1 .5


1 .5

0


1

Total


68


84




38

This data shows that post-chlorination (O.8 ppm)  caused an
increase of TTHM from 68 ppb to 84 ppb during the summer
season.

The formation potential of the raw water was 16O ppb after
48 h at 2O °C.

-------
                          - 228 -
TKBUB II THM in Kralingen Plant'
         Coag. 2.5 ppm ozone-filtr.  (GAC)





o
o

CM
t
i






O
o
T



Raw water
pre-chlorinated
(5 ppm 15 h)
after Floe Separation


after Ozonation

Finished water
(without GAC)
(O.8 ppm 6 h post-chlorin.)
Raw water

not chlorinated
after Coag.+ Ozone


eluted from GAC
(7 -1O months in use)
Finished water
(post-chlorinated)
CHC13

50

32


32



49

' 1
I
1


5 ,
6.5

CHCl2Br

32.5

22


-23'



35 .


'
0


3
6.5

CHClBr2

18

13


12



16.5



O


1 .5
9.5

CHBr3

2.5

1 .5


2



1 .5



0


0
6.5

Total

103

68


69



102



1


9.5
29

TABLE III  THM in Kralingen Plant
           Influence GAC

after GAC (first month)
after GAC post-chlorin.
(O.8 ppm 6 h)
after GAC (2-5 months
in use)
after GAC post-chlorin .
CHC13
1 .2
3.2
17
18
CHCl2Br
0.8
3.7
11
12.5
CHClBr2
O
5.4
4
13.5
CHBr_
0
5.7
O
8.5
Total
2
18
32
52

-------
                          - 229  -,
In the winter period when only breakpoint chlorination
with a chlorine dosage of 4 ppm at a contact time of
3O min. was applied, the finished water contained 38 ppb
TTHM.
It is interesting to compare this figure with the results
of the ozone-GAC treatment in the Kralingen plant.

Results of new treatment plant

In the Kralingen plant the -reservoir water is coagulated
with iron, followed by ozonation  (3 ppm), dual media fil-
tration and final filtration through activated carbon
(GAG, contact time 12.5 min.).

The plant was started in July 1977, when the raw water was
pre-chlorinated. During 15 ;h transportation TTHM went up
to 103 ppb. In the first month of operation the carbon
filters could not be used, 'because they were in the pro-
cess of being filled.

The effects of this treatment coagulation and ozonation
without carbon are shown in Table II. It is seen that the
THM once formed by pre-chlorination is only partly removed
by the coagulation and ozonation steps. An interesting ob-
servation is that post chlorination of O,8 ppm gave rise
to renewed THM production, i.e. an increase from 69 to
1O2 ppb. The TOC value after .coagulation ozonation was
2.8-3 ppm.               '

The advantageous effect of the adsorption of both pre-
cursor and THM onto fresh carbon may be seen from the
data in Table III, upper lines. The TTHM in the raw water
still amounted to 1OO - 1O3 ppb. Adsorption reduced this
to 2 ppb.  The observation that post-chlorination (mea-
sured after 6 h) produced 18 ppb TTHM, as compared to
33 ppb without GAG, indicates that precursors remaining

-------
                          - -23O - '


after ozonation were partly reduce.d-. Ouringr this- first
month of operation the carbon  filter treatment., lowered
TOG from 3 ppm to O.9 ppm. In  the next  three months, how-
ever, the TOC of the effluent  gradually rose to 1.5 ppm.

Dufring this period THM breakthrough started to become
significant for CHC13   (Table  III, lower case).  The more
brominated THM were withheld"longer._

Precursor removal was still performed at a slightly di-
minished level. Post-chlorination now caused a 2O ppb
increase of TTHM  (32 to 52 ppb).

During the following winter season the  pre-chlorination
was interrupted and consequently THM formation did not
occur. The results of the ozone - GAC - post-chlorination
treatment are given in the lower case of Table II.

Two conclusions may be drawn.  First, the TKM orginally
adsorbed was slowly eluted, resulting in average values
over the 4 winter months of 9.5 ppb TTHM. Secondly, pre-
cursor removal appears to remain at about the  same level  .
as indicated by the 2O ppb increase caused by  post chlo-
rination.
However, the effect of lower temperature on the  overall
reaction rates have to be taken into account.

Table IV summarizes the observations reported  here.
The data clearly show that pre-chlorination of raw water
is deleterious to any further  conventional or  advanced
treatment. Starting with non-chlorinated raw water j_n the
conventional treatment resulted in 38  ppb TTHM, as compared
to 2O ppb TTHM increase during 6 h post chlorination. In
this case the total effect was negatively influenced by
the elution of 9 ppb TTHM resulting from the preceding
period during which the raw water had been chlorinated.

-------
                          _ 23-t'-


A final-observation'is'that' o z One ' treatment  resulted  in  a!''
shift towards brominated THM in post chlorination,  as shown
by the data of the  finished waters of  the conventional
treatment  (Table I, last line) vs. new treatment  Tables  II
and III.
TABLE IV
TTHM
PLANT I
Conventional
ppb
Raw water ,
not chlorinated

Finished water 38
Raw water go
prechlorinated

Finished water 84


TTHM
PLANT II
Ozone GAC

Raw water
not chlorinated
Coag . -Qzone-GAC
(6-10 months)
Finished water
Raw water
prechlorinated
Coagulation-Ozone
Coag. -Ozone-GAC
Finished water
(O.8ppm post chlorinated)



ppb
1
9
29
103
69
32

52

-------
                           - 2 3 2 • - -


 CHEMICAL CHANGES AND -REACTION .PRODUCTS.,,IN ,-THE .OZONI^TIQN
 OP ORGANIC WATER CONSTITUENTS -   -

 E.  Gilbert
 The  growing  interest in the use of ozone for the treatment
 of drinking  water and waste water has led not only to
 studies  on the  process techniques for the ozone input (1-10),
 the  mass transfer from gas- .to water phase (11-13) ,  and the
 behaviour of ozone in water (14-203, but also to a large
 number of publications on the  chemical effect involved. The
 following is- a  survey of the reactions of ozone with the
 organic  constituents of water  and of the oxidation products
 produced in  this  way.


 Disinfectant effect

Until the nineteen-fifties ozone was used mainly for its
disinfectant effect;  it had been used in the treatment of
drinking water as early as the turn of .the century (21), and
therefore the majority of publications have concentrated on
this partial aspect.  In addition to more recent results on
its disinfectant action and on viral inactivation by ozone
 (22-27),  attention is particularly drawn to the proceedings
of the 1976 Chicago  conference "Forum on Ozone Disinfection"
 (28).

Since the analysis methods are extremely complicated, very
little can be said about the mechanism of disinfection or
the inactivation of  viruses, which can often take place in
a matter of seconds  with a dose of 1 .mg 0^/1.  The first
insight  into the chemistry of the disinfection processes was
provided by trials on amino acids, the building blocks 'of
proteins, in pure solutions. Mudd et al.(29),  Menzel (3O), and

-------
                          - 233 -
Shuva'l '•'(3'l)'-r-re'po£ated -that  the'; -unsaturated ' ami'no acids such' '-'4 •'
as tryptophan, tyrosine, histidine,  and the sulphur-containing
amino acids such as methionine,  gluthathione, cystine, and
cysteine are readily attacked by ozone.  Shuval (31) found
that viruses react with ozone at much the same rate as the
above-mentioned amino  acids  (Pig.  1, ozonization of trypto-
phan) .  This is an indication that the inactivation of
viruses may be due to  an attack  of ozone on these amino
acids and that as a result of oxidation the acids lose
their structural characteristics important for biological
activity.  In the case of  the unsaturated amino acids, such
as tryptophan, the double  bond is  attacked and the molecular
structure is destroyed (Fig.  2).   In the sulphur-containing
amino acids the sulphur is oxidized to sulphoxide, sulphone
or to sulphonic acid,  whereby the acids can no longer act
as oxygen carriers in  redox  processes. It is not known
whether the oxidation  products themselves have any disinfec-
tant action, since their identification has been only partial
and qualitative.
 O
 E
 3,
 c
 a
 a.
 o
l_
    0,5
03=1,8-2.0 /u mole/sample
Sample volumes = 5,0 ml
Temperoture =0 "C
                           Fig. 1
                           Reaction of tryptophan with
                           ozone;  dependence on the pH
        15 30   60  90   120
                Time [ S ]

-------
    Ami no acids
    Tryptophon
               COOH
        ~*C — CrurCH
        *   *
       H
                           -•234 -
 Oxidation  products
 Kynurenine
    9    ,COOH
  -i-C-CH-rCH
OJ     2 N
 N-Formylkynurenine
    9      DOOM
    ~ C* H -^"C H
          NH
    Methionine
    CH3- S - CH2-CH2-CHlNH2)COOH
                                              NH
 Methionine sulphoxide
CHjS-CHjCHj-CHlNHjlCOOH
   6
    Cystine
     S-CH2-CHiNH2)COOH
  Cysteic acid
H03S-CH2-CH(NH2)COOH
Fig.  2   Ozonization  of  amino acids; oxidation products
Effect, on the sum parameters
Besides the disinfectant effect of ozone which, as already
explained,  may be associated with the  ready oxidizability of
amino  acids, its other  beneficial effects have been utilized
for a  considerable time.   These are .an improvement of  the
water's odour and taste (32-36), removal of any colour or a •
reduction of UV-extinction (32, 37-45),  as well as a
reduction in the molecular weight of high-molecular sub-
stances (46-50).   Fig.  3 shows as an example the effect of
ozonizing humic acid from Hohlohsee  (Northern Black Forest)
on the size of its molecule.    After a dose of only
1.5 mg 03/mg C,  half of the carbon is  present in the form
of molecules with molecular weights below 1000 (50).

-------
                         - 235 -
The DOC and the COD  are also decreased by oxidation processes,
It is found that  the DOC decreases much more slowly than  the
COD and that with - longer ozonization times both approach
asymptotically to a  final value.   To illustrate this point
the results obtained in the ozonization of 2-nitro-p-cresol
in.water are shown in Fig.  4.  An  unbuffered 1 mmol solution
at pH 5 was used.  The greatest decrease in the two par-
ameters occurs after degradation  of the cresol, i.e. the
products formed are  present in a  higher oxidation state
and  -  at least in the acid range -  react further with
ozone only very slowly.
  70

  60

— 50
o
f 40
o
g 30
   20

   10
I
               %
               I



                              1

                               I
                                           M¥
                                           MW

                                           MW
                                           MW
                                                > 30 COO
                                               000- 40 OOO
                                                 < 4 000
         0      0,31      0,6     1,47      2.1
            Ozone coasumpiion,mg/mg  starting DOC
Fig. 3  Ozonization of huruic  acid  (Northern Black Forest)
        without the molecular weight fraction > 1OOO

-------
                         - 236 - ',
                     60            120            180
             Ozonization time (min)  dooe 10 mg Oj/mirvl
 iq.  4  Ozonization of 2-nitro-p-c.resol  (c =  1 mmole/1)
        TOG and COD decrease in dependence on  the
        ozonization time
In a further example, which  is  representative of many (38,
39, 43-45, 48, 51-69, 120, 134)  a  paper  pulp waste water
was studied  (51).  As Fig. 5  shows,  with a limited ozonization
time a total degradation to  C0~  and  H-O  is not achieved.
The extent of the COD reduction  naturally varies from one
substance class  to another,  and  in the case of a given waste
water reacts according to  its composition.   After the COD
has been decreased by 50-70%  further ozonization brings
about only a negligible reduction  of this parameter.

According to the present data,  a reduction of the COD value
by 1 kg is attained by the consumption of 1-4 kg of ozone
(Table 1), and in extreme  cases  even 6-10 kg of ozone'may
be necessary.

-------
                            -  237 - •'
  1100
^900
OT
E
CD
CO
o
* 700
  Vol. of effluent used' 10 I

Ozone dose 1,63 g/l
           246
           Ozonization time [h]
Fig.  5
Ozonization of paper mill
effluent; COD decrease in
dependence on Ozonization
time
                       10
TABLE  1   COD degradation and  ozone consumption
Solution used
Ca ligninsulphonate
Pacer mill effluent
Effluent
Industrial effluent
Effluent from a sauerkraut factory
Industrial effluent
Paper mill effluent
Discharge from clarification plant
Discharge from clarification plant
Discharge from clarification plant
Discharge from clarification plant
Humic acid solution
Naphtbol
Phenol
Chlorophenols
Haphthalene-2.7-disulphor.ic acid
p— Toluenesulp!:cnic acid
COD 	 COD
mg 02/1
1130 — » 890
4950 -j. 2500
225 — > 198
3340 — *• 1930
3340 -*. 314
2140 -» 1620
910 -» 150
14000 -» 3000
156 -* 94
1480 -* 1150 -
59 -> 49
59 -> 35
58 -» 50
48 ~> 24
43 -* 42
56 — » 32
80 ~> 16
20— > 10
240 — » 60
26 —» 19
70 — > 24
216 -* 106
265 — > 60
250 — > 60
Dzone consumption
A COD
1
1.8
1.5
0,8
1.8
2.5
5.0
2.C
0.7
1.4
1.4
2.0
1.5
2.5
5.6
1.7
2.5
2.5
2.7
ir
1.6
1.5 - 2.3
2.5
3.7
Ref.
51
38
57
62
66
44
48
60
58
63
52
61
72
54
—

-------
                          -  238,--
 With  small  ozone  doses  an increase  of  the COD  has  actually
 been  observed  in  some cases  (43,  61, 64,  68,  69).   In  the
 first phase of the  ozonization  some substances are made
 accessible  to  the COD analysis  by the  oxidation,  thus  pro-
 ducing an increase  in COD which then decreases again as the
 ozonization continues.

 The biological degradability  is often  improved by  the  attack
 of ozone, as has  been reported  in many recent  papers.   Data
 on the rise  of  the BOD5/CCD ratio after ozone  treatment are
 found particularly  in (38, 44,  45,  54,  65,  70-72).'
The change in the molecules after ozonization also in-
fluences the chlorination processes in the treatment
of drinking water. Preliminary ozonization reduces the
formation of chloroform during the subsequent chlori-
nation  (73-77). Maier  (75) observed that as the chloro-
form decreased an equivalent  increase of bromodichloro-
methane and dibromochloromethane took place in ozonized
Lake Constance water.  Lawrence (77) was able to establish
that in the ozonization of ligninsulphonic acid and aspartic
acid the concentration of "chloroform precursors" increased
at the start of the reaction and only fell again as the
oxidation progressed.  Rook (73)  found in addition that the
ozonization effect contributing to the reduction of chloro-
form formation becomes the smaller, the longer is the
interval between ozonization and chlorination.

In the large-scale plant at Lengg  (Switzerland)(78)  increase
in the concentrations of two halogen compounds,  dichloro-
butanone and iododichloromethane,  could be observed after
ozonization.  Block and Buydens found an increase of the
chlorine demand after ozonization of raw water (79,  80).
Maier (75)  showed that after ozonization of Lake Constance
water the chlorine consumption proceeded essentially more

-------
                         - 239 -


slowly than in non-ozonized-water.  In addition, several
authors have described an increased repopulation with
bacteria due to ozonization  (75, 78).

Finally, the ozonization of raw water or river bank filtrate
can lead to the formation of non-polar chlorine compounds
 (81).

The effects mentioned depend of course on the type of the
raw water or on the composition of its constituents.  The
latter, however, are so numerous that so far the complex
systems of raw water and waste water can in effect only be
characterized with the aid of sum parameters.  Therefore,
the data concentrate on the action of ozone on such measur-
able parameters.

Ozonization of pure compounds
To explain and thereby also to control these phenomena, a
knowledge of the exact compositon of the aqueous solutions
would naturally be necessary.  This is the first basic pre-
requisite for informative results.  It  also seems useful,
as in the case of viral inactivation, to resort first to
model substances and to study the ozone-oxidation on simple
systems.  This approach has been described in recent years
by several working groups.

Quinoline
Naimie (82)  ozonized 200 ml 'of a 0.25 - 0.5 mM quinoline
solution (pH 6.9), which after 60 min (30 mg O3/min) con-
tained less than 10% of the initial quinoline content.

Cyclohexanol, cyclohexene
Cyclohexanol and cyclohex-l-ene-3-ol (2 g/1)  in the presence
of 10.5 g of Ca(OH)2 were eliminated with 4 g/1 or 1.5 g/1
of ozone.  Malonic acid,  glutaric acid,  adipic acid, and

-------
                         - 240

succinic acid were identified quailita'tiveiy 'amoncj the oxid-
ation products, but amounted only to an estimated 5% of the
total amount of the oxidate  (83).

Urea
Eiehelsdo'rfer  (84) was able to determine quantitatively the
oxidation products of the ozonization of urea, CC>2 and nitric
acid.

Malonic acid
The ozonization of malonic acid  (85, 54) produced tartronic
acid, mesoxalic acid, oxalic acid, £®2' an<^ H2°?'  A mass
balance was set up.  In this process 192 ppm of ozone are
consumed in 100% elimination of  104 ppm of malonic acid.

Maleic acid
Black (86) ozonized 40 g of maleic acid in 100 ml of water
with the stoichiometric quantity of ozone for the prepar-
ation of glyoxylic acid.

Caffeine
Shapiro (87) found in the ozonization of caffeine (660 ppm)
that 4.2 moles of ozone were necessary to eliminate 1 mole of
caffeine.  Dimethylparabanic acid was identified in addition
to several other oxidation products.

Dichlorobenzene
The ozonization of dichlorobenzene in pure aqueous solutions
(88) proceeded most efficiently  at pH 8.4.  60 mg of ozone
are needed to oxidize 30 mg of the substance/1.

Glucose
Walter (89) described the reduction in the COD after ozon-
ization of 6 litres of 0.1% glucose solution.  After a maxi-
mal dose of 1.6 g of ozone the COD fell by 10-15%.

-------
                         - 241 -

Ethylenedi.amine,t,etraacetic acid ,
Krause  (90) studied the decomposition of ethylenediamine-
tetraacetic acid chelates with ozone.  To release 90% of the
metals  (Ca, Zn, Ni) from the chelate complexes in 1 litre
of 0.01M solution an ozone amount of 1.7 - 3.4 g 0-,/l was
used.  The reaction proceeds from pH 7 in the basic region
twice as rapidly as in the acidic region.

Aliphatic alcohols
The ozonization of methanol  (91, 91a, 92), ethanol, butanol,
and octanol (92) proceeds via the aldehydes to carboxylic
acids.  88% of isopropane is transformed into acetone  (93).
In the ozonization of ethanol, Gilbert (94) found in addition
H2O2, short-lived peroxides, and formic acid  (Table 2).

Alkylbenzenesulphonic acids
Buescher and Ryckman  (95) investigated the behaviour of
tert.alkylbenzenesulphonates  (ABS) with respect to ozone.
12 mg of ozone were consumed to oxidize 4.5•mg of ABS. Evans
(96)  found that the removal of 15.6 mg of ABS in 1 litre of
discharge from a biological clarification plant requires an
ozone dose of 75 mg/1, and that the oxidation products are
utilized better biologically.  Kandzas (97) reports that at
pH 11 90 mg of ABS are eliminated by 190 mg of O-j.  Small
quantities of formaldehyde and formic acid were found as
oxidation products.   Joy  (98) studied the ozonization of
nonyl-  and decylbenzenesulphonates.  720 mg of CU were
needed  at pH 6 to degrade 160 mg of sulphonate and only
240 mg  at pH 12.  70% of the sulphonic acid was converted
into sulphate.  Formic and oxalic acids were determined
quantitatively and nonylglyoxal qualitatively as organic
oxidation products (Table 3).

-------
                          - 242 -
  TABLE 2  Ozonization of alcohols
Compounds
Methanol
Ethanol
Butanol
Ethanol
Isopropanol
: 	
Concentration
140 mg/150vm1
210 mg/150 ml
330 mg/150 ml
92 mg/1
192 mg/1
Ozone
consumption
90 mg
360 mg
380 mg
211 mg
amount added
2.6 g
Degrad-
ation
29 %
71 %
64 %
90 %
100 %
pH
5.5 - 5.5
5,4 - 3,8
6.2 - 3.7
6.3 - 3.8
7
Ref.


92
94
93
  TABLE 3  Ozonization of alkylbenzenesulphonates
Compounds
Alkylbenzenesulphonate
11
H
Nonyl- and decyl-
benzenesulphonate
Concen-
tration
4.5 mg
15.6 mg
90 mg
160 mg
160 mg
Ozone
consumption
12 mg
75 mg
190 mg.
720 mg
240 mg
Degra-
dation
100 %
100 %
100 %
100 %
100 %
PH


11
6
12
Ref.
82
83
84
85
Polycyclic  aromatics
Reichert  (99) and Cornelia  (100)studied  the degradation of
3,4-benzpyrene.  0.7 ppm of ozone are required to  remove
1 ppb of benzpyrene from water.

Chlorine-containing pesticides
The Ozonization of pesticides has been  studied by  several
authors (Table 4).  Up to now the toxic dieldrin has been
identified qualitatively as the oxidation product  from
aldrin and heptachloroepoxide from heptachlor (101).
Brower (105) established in the fish test that the oxidation
products of aldrin are less, toxic.

-------
                            243 —
  TABLE. ,_4   Ozonization of chlorine-containing pesticides
Pesticide
Lindane
Endosulf&n I
Endosulfan II
Dieldrin
Heptachl oreepaaft
DDT "
Aldrin
Heptachlor
Lindane
Lindane
Lindane
Aldrin
Aldrin
DDT
Concentration
2 ppm
ti
i»
H
II
M
11
"
40-100 ppb
10 ppb
50 ppb
20 ppb
0,005 %
suspension
7 ppp .
Ozone
dose
17 ppm
M
II
It
li
11
It
It
0.4-3 ppm
11 ppm
149 ppm
23.8 ppm
2.85 g

Ozone
con-
sumption










97 ppm



Degra-j
dation
0
0
12 %
26 %
26 %
78 %
86 %
iop %
0
10 f;
100 %.
100 %
100 %
100 %
pH










9.8/6,8

7.5-4,4
'7,9-4.5
Ref .



101




102
103
104
105
112

Phosphorus-containing pesticides
In contrast to the chlorine-containing pesticides, the
phosphorus-containing ones are more readily oxidized.
Richard and Laplanche (106-108) found that after an ozone
dose of 3 ppm 80 ppb of.parathion were oxidized?.toxic para-
oxon is formed among other products,'and is degraded only
after 5 ppm of ozone has been added.  2,4-Dinitrophenol,
picric acid, sulphuric acid were identified qualitatively
and phosphoric acid quantitatively as" the oxidation products
(Table 5).

Waste water constituents
For a better control of the ozonization of waste waters from
photographic processing laboratories,  the individual consti-
tuents have also been studied  (59,109), such as acetic acid,
glycine, diethylene glycol, benzyl alcohol, and various
colour developers.  The concentrations amounted to 1 g/1 and

-------
                           - 244 -
        5  Ozonization of phosphorus-containing pesticides
Pesticide
Malathion
Parathlon
Methyl pa ra th i on
Fenitrothion
Parathion
Fenthion
Concentration
100 ppb
S7. ppb
125 ppb
120 -ppb
2500 ppb
2450 ppb
Ozone
amount
5 ppm
"
it
11
149 ppm
149 ppm
Degra-
dation
88 %
• 83 %
m %
• 93 %
100 %
100 %
PH
8



9,8/6.5
9.8/7.2
Ref.
106-108
11
11
II
104
 the ozone dose to 0.5 g O3/h.  Ozonization was performed
 for 8 h and the COD decrease was measured.  Glycine and
 acetic acid were the only substances tested that exhibited
 no change.

 Bauch and Burchard (110)  studied a series of organic sub-
 stances and determined only the degree of degradation.   The
 concentrations used were  1-2 g/1 for raethanol,  ethanol,
 glycerol,  ethyl acetate,  acetic acid,  caprylic acid,  sugar,
 and glucose and 0.1 - 0.5 g/1 • for- hydrazine,  phenol,  o-
 cresol,  hydroquinone,  o-salicylic acid,  pyridine,  benzine,
 chloroform,  benzene,  and  toluene.'  Acetic acid,  ethyl acetate,
 caprylic acid,  pyridine,  and chloroform were  shown to be
 stable  to ozone.

 Caulfield  (111)  established  by  the Ames  test  that  the muta-
 genic action of  various policyclic aromatic hydrocarbons,
 aromatic amines, and  some pesticides can  be eliminated  by
 oxidative degradation with ozone.   However, the primary
 oxidation products  of benzidine had stronger  mutagenic
 action  (111,112), though on  further Ozonization they  were
degraded to  inactive products.

Spanggord (112) studied the Ozonization of 30 organic com-
pounds in high concentrations to obtain large concentrations

-------
                         - 2 4-5 ' -
of intermediate 'products'.''" "The mutageriicity"of these was
then investigated in the Ames test  (113).
The compounds in question were 2,4-dinitrotoluene, diphenyl-
hydrazine, acetic acid, aroclor 1254, glucose, urea, chol-
esterol, benzidine, glycine, cysteine, benzene, thymine,
caffeine, diethylamine, phenol, hydroquinone, glycerol, and
nitrilotriacetic acid in up to 1% solutions.  Only the
ozonized solutions of phenol,' 1,1-diphenylhydrazine, nitrilo-
triacetic acid, benzidine, and ethanol were shown to be
mutagenic after ozonization.  A selection of the compounds
used is listed in Table .6.

 TABLE 6  Ozonization of organic substances
          after Spanggo'rd (112) and Simmons (113)
Compounds
2 , 4-dinitrotoluene
Oleic acid
Acetic acid
Glycine
Ethanol
Benzidine dihydro-
chloride
Phenol
Concern
tration
• 80 -pprti
,99 ppm.
1263 ppm
11900. ppm
7759 ppm
4830 ppm
9140 ppm
Ozone
amount
1.7 g
2.55 g
37,2 g
9,8.g
55,8 g
2^25 g
6,97 g
Degra-
dation
55 %
100 %
3,5 %
22 %
74 %
64 %
41 %
Mutagen-
icity
before
03
—
--
—
'--
--

Mutagen—
icity
after Q3
--
--
--
--
+
+
+
Salicylic acid           •
Mallevialle (52) established in the ozonization of salicylic
acid (100 - 200 mg/1) with degradation of the starting com-
pound an increase of the extinction at 420 nm, which, after
reaching a maximum fell to zero with the elimination of the
salicylic acid.  Thin-layer chromatography revealed the
presence of other phenolic oxidation products not identified
more closely.

-------
                         - 246 -
Phenols
Hillis  (61) investigated the degradation of various substi-
tuted phenols  (30 mg/1) at pH 4-10.  It was found that not
until pH 10 did the degradation proceed twice as rapidly as
at pH 4 and 7.  To achieve a degradation below 0.1 mg/1 the
same ozonization time was necessary in all cases.

Dorc (114)  found that during the ozonization of aminophenols,
nitrophenols, and halophenols and of dichlorophenoxyacetic
acid (25 mg/1) UV-active oxidation products were formed in
the first phase of the reaction.  The extinctions of the
new absorption bands pass through a maximum and finally fall
to zero after 100% degradation of the starting compound.

Bauch (115) was able to identify qualitatively the following
oxidation products in the ozonization of cresol, chlorophenol,
and xylenol (3.8 g/200 ml)jglyoxylic acid, acetic acid,
propionic acid, maleic acid, glycolic acid, oxalic acid, CO-*
and IIC1.  The degradation rate was independent of the pH.

                                       -4
2,4,5-Trichlorophenoxyacetic acid  (c=10  M) was oxidized by
ozone to chlor'ide, glycolic acid, oxalic acid, glyoxylic
acid, and dichloromaleic acid -(116) .  The oxidation products
were determined quantitatively.

The unidentified oxidation products of nitrophenols and
phenol were shown in the mouse test to be non-toxic (117,
118).

The reactions of phenol with ozone have so far been studied
in the greatest detail.  Several working groups have deter-
mined the decrease of the phenol in the course of ozonization
(119-122).   The results on the pH-dependence reveal that the
oxidation takes place more rapidly at pH 10-11 and the ozone
consumption is only half of that in the acid region (119)
(Table 7).   Eisenhauer (123-125), in the ozonization of

-------
                          - 247- --
  TABLE 7  Ozonization of phenols
Compounds
Salicylic acid
Phenolsulphonic acid
Chlorophenols
Amino phenols
Nitrophenols
Cresol
Xylenol
Naphthol
Amino, nitro, and
halophenols
Cresol, chlorophenol ,
xylenol
Chlorophenols
Dichlorobenzene
Phenol
Phenol
Ozone consumption until the
- phenols fall below the detection
level
3 moles D.,/1 mole acid
4.9 moles O,/mole phenol
(mean value) .
5 moles 0-,/mole phenol
5.5 moles 0,/mole phenol
3-4.5 moles O-,/mole phenol (pH 5)
6 moles O.,/mole benzene
4 moles 0-,/mole phenol (pH 7)
2 moles O3/mole phenol (pH 12)
6 moles O,/mole phenol
4-6 moles O-,/mole phenol
Ref .
52
61
114
115
72
88
119
122
127
phenol solutions  (50 mg/1), obtained practically  the  same
rate of degradation until  pH 9.  Only at pH  11 was  the
degradation rate doubled.  Pyrocatechol quantitatively  and
o-quinone qualitatively were identified as the first  oxi-
dation products.
Reissaus (35) identified oxalic acid qualitatively as one of
the oxidation products..  Casalinl  (126) ozonized phenol
solutions with 500, 1400, and 5000 mg/1 in a 3.75-litre
container (ozone doses 23/26 mg O3/min) for 40 h at pH 3-11.
Pyrocatechol and hydroquinone were determined together

-------
                         - 24-8 '-''-  '

quantitatively as'the oxidation .products and maleic acid,
formic acid, glyoxa'l, formaldehyde, and oxalic acid were
found to be present.  Gould (127) studied phenol solutions
(90 mg/1, pH 7.7) ozonized for periods of up to 30 min
(ozone dose 72 mg O3/min).  Pyrocatechol, hydroquinone,
glyoxylic acid, and oxalic acid were recorded quantitatively
as oxidation products during the o'zonization.  On the basis
of the carbon balance these are the only reaction products.
On the other hand, Niki  (128)  found formic acid to be one of
the main products of the ozonization of phenol.  In-this
case 57 mg of phenol in 100 ml of water were treated with
only 5.8 mg 03/min for 180 min.  Muconic acid, muconaldehyde,
maleic aldehyde, glyoxylic acid, glyoxal, and oxalic acid
were identified quantitatively as further oxidation products,
making up 100% of the TOC.

Substituted aromatics and their oxidation products
The different results obtained by the various working groups
can be explained by their use of different reaction condi-
tions such as the initial concentration, dose of ozone,
ozonization time, pH, an'd reaction volumes or reaction
vessels.  Furthermore, the different rate constants are
responsible for  the formation and concentration distribution
of the oxidation products.  Fig. 6 shows that the unsatu-
rated aliphatic  carboxylic acids are degraded more rapidly
than the substituted aromatics.  Therefore,  after their
formation, they  can be immediately oxidized  further in
competitive reactions with the starting compounds.

For example, with low ozone doses and high initial concen-
trations the chances of being able to isolate unsaturated
aliphatic carboxylic acids such  as fumaric or muconic as
the oxidation products are higher than in the case of large
ozone doses and  low initial concentrations;  this can be seen
on the example of the ozonization of phenol  under various
conditions  (126-128).  For a better understanding of the

-------
                           -249  -,.,-
                                         .fenzoic acid
                                       Glyoxylic acid
                                  Oxalic.arid aesoxalic acifls
                           \
                                 Muconic acid
                             \       \Phenol
                        .Dichlorophenol      *v
           5     10     15     20    25     30
               Oeonization time (min) , dose IQmgOj/mirvl
Fig. 6   Degradation of aliphatic and  aromatic compounds in
         the  ozonization of -aqueous  solutions, ozone dose
         1O mg
         c   = concentration at ozonization time t
         c   = initial concentration  1 mmole/1
         o
course of the  reaction during ozonization  it  is  necessary
to know the rate  constants.   Hoigne' has contributed much to
this problem  (129,130).   It appears that in the  acid range
the ozone molecule is directly responsible for the oxidation
reactions, while  in the basic range the decomposition product
of ozone formed by OH  ions,  the OH radical,  initiates the
oxidation by addition and abstraction reactions.
In the light of  the  work presented, the ozonization of sub-
stituted aromatic  substances can be represented as follows,
2-6 moles of ozone are necessary for the elimination of
1 mole of the aromatic initially present  (Table 7).  In the
initial phase of the reaction (up to about  50%  degradation)
an increasing  coloration (pink or yellow)  of the  solution

-------
                            250  -
is observed or a new UV extinction is measured.  The colour
intensity and/or the UV extinction reaches a maximum on
elimination of the starting compound and then falls to zero
(52, 114, 116, 119, 128, 133, 134).  These observations
indicate that the first step in the ozonization of substi-
r
tuted aromatic substances need not be the spontaneous ring
opening but that the primary oxidation products -  it is not
known to what extent they are formed  -  have an aromatic
character or a six-membered ring structure.  These primary
oxidation products could be, for example, hydroxylated or
quinoid compounds, such as have so far only been identified
in the ozonization of phenol  (as quinone, hydroquinone, and
pyrocatechol) (123, 126-128) .

If ring cleavage then occurs by further attack of ozone or
OH radicals, muconic acid derivatives can be formed, which
are transformed via maleic or fumaric acid derivatives into
carbonyl and/or carboxyl compounds with one to three carbon
atoms.

The course of these subsequent reactions has been studied on
model compounds such as muconic, maleic, and fumaric acids
and their oxidation products (54, 131, 132).  All the com-
pounds (Table 8)  could be followed quantitatively, so that
reaction mechanisms may be proposed.

The oxidation products of the aliphatic model substances
were also found in the ozonization of substituted aromatics
and phenols  (Fig. 7).  This indicates that after ring
cleavage further oxidation takes place via muconic or
fumaric acid derivatives.

After ring cleavage the substituents are converted into their
mineralized form (54, 72).  The detection of dichloromaleic
acid (Fig. 7) (116) shows that the hetero-groups do not

-------
                          -  251  -
 TABLE  8   Ozonization of  aliphatic carbbnyl and
           carboxylic  compounds.
           Initial  concentration  1  mmole/1;  ozone dose
           10 mg  03/min 1;  ozonization time  2O-18O min;  pH 3
Compounds
trans, trans-Muconic acid
Formic acid
Glyoxylic acid
Maleic acid
Fumaric acid
Glyoxal
Tartronic acid
Malonic acid
Dihydroxyfumaric acid
Oxaloacetic acid
Oxidation products
Fumaraldehyde, glyoxal, glyoxylic
acid, oxalic acid, formic acid,
C02, H202
C02, H20
Oxalic acid, CO_
Formic acid, glyoxylic acid,
oxalic acid, CO-?
Formic acid, glyoxylic acid,
oxalic acid, ' mesoxalic acid
aldehyde, mesoxalic acid CO2
Glyoxylic acid, oxalic acid,
C02
Mesoxalic acid
Tartronic acid, mosoxalic acid,
C02, H202
Oxalic acid, hydroxytar tar ic
acid, CO2
Formic acid, glyoxylic acid,
oxalic acid, mesoxalic acid,
co2
Ref.
132
131
54
11
It
II

always hydrolyse spontaneously after ring cleavage and are
not always converted completely into the inorganic form.
Depending on the substituent, many further aliphatic oxi-
dation products that contain hetero-groups are possible, but
so far they have not been identified.  Even in the case of
p-toluenesulphonic acid  (135), 2-nitro-p-cresol  (135), and
4-chloro-o-cresol  (54) so far only the oxidation products
given in Fig. 7 could sometimes be followed quantitatively
in the course of the ozonization  (Table 9).

-------
                          -  252  -
  61
 HO

SQjH
                     CH3
                      , NH3
^.0
H-C'
H-C*°
ov
K'
o
"H
o
H'
»°
"OH
0
HO'
* 0
c-c' <
OH
0
:H3'C^
OH
H
HO-C-C
H
0
"OH

CH3-

o
c-c:° i
H
.
1 ,0
OH

D i 0 Q i
H' OH HO

°
OH
                      Cl &       0« OH
   0  H H  0  «  MHO  0. I  I  ,0   0  , I   0
   *c-c-c-c   *c-c-c-c    *c-c-c-c*   *c-c-c-c*
  HO     VH  HO      OH HO     OH HO     XOH
       0  MKMM^O    'Q^HUHH^C
                               Fig.  7
                               Oxidation  products
                               identified so  far
                               in the ozonization
                               of substituted-
                               aromatic substances
By  comparing the TOC calculated from the measured products
with  the  directly determined TOC (Figs. 8-10), it can be
seen  that at the beginning of the ozonization only a small
part  of the  oxidation products (24-60%) was included.  The
maximum of the  deficit lies at 90-100% elimination of the
starting  compounds.   At this time 1.5 - 5 ing of 03 are used
up  per mg of the initial C-value.  Only after higher ozone
consumption  can 60 - 80% of the oxidation products be
detected  quantitatively.  As mentioned previously, the still
unidentified products after 90% elimination of the starting
compound  may be compounds with a 6-membered ring structure.
The first attempts  to  characterize these products showed
that up to  90% degradation of  the initial compounds the
BOD^/COD ratio was  0.1 and lower (134).   The ozonization of
aniline may be quoted  as  an example (Fig. 11).   After 90%
degradation the  solution  has a reddish colour.   The BOD5/COD
of 0.64 for the  non-ozonized solution falls to  0.01 after

-------
TABLE  9  Ozonization  of substituted  aromatic  substances  (pH  5 - 2.5).
          Initial concentration 1  mmole/1; ozone dose  24 mg O3 min  1
          for  the p-toluenesulphonic  acid; 10  mg O3/min 1 in  all
          other cases.
   Compounds
p-Toluenesulphonic acid
    Oxidation products
                                                             Fraction  of products in TOG, %,
                                                                  after 90% degradation
                                                       for the starting
                                                    ]   compound
, Methylglyoxal, acetic acid,
| pyruvic  acid, formic acid,
.oxalic acid, C0~, H-09 ,

I I!2S04
                            64%
                            after 5.7  mg  03/tng C
                            9.9 mole.03/mole PTA
2-Nitro-p-cresol
Methylglyoxal, glyoxylic    j 38%
acid,  acetic acid, pyruvic   after 2.5 mg 03/mg C
                            4.4  mole 03/mole NC
 acid,  formic acid, oxalic
                    3
                         acid,  C02,  H2°2' HN°
                                                     after higher
                                                     ozone dose
79%
after 8 mg 0.,/mg C
                                                  68%
                                                  after 6.9 mg  0,/mg  C
                                                                              to
                                                                              in
                                                                              w
4-Chloro-o-cresol
 Methylglyoxal, acetic acid,
 pyruvic acid, formic acid,
 oxalic acid, CO,,, HC1,
 H2°2
                            24%           •
                            after  1.3 mg  03/mg C
                            2.3  mole 03/mole CC
65%
after 3 mg  0-,/mg C

-------
  80
  50
o
en
E
  20
                      TOC measured
                                     I  I 1
            50       100      150

              Ozonization time  [min]
                          200
Fig.  8   Ozonization  of p-toluenesulphonic acid,
         carbon balance

         c = 1 mmole/1;  ozone dose 24  mg O3/min 1; pH  3
  80
  60
o
01 40
E
  20
                 TOC measured
50
                   100      150      200
                    tine [min] IOmgOg/min-1
Fig. 9  Ozonization of  2-nitro-p-cresol,  carbon balance

        c  = 1  mmole/1;  ozone dose 1O mg  03/min; pH 5.5

-------
  80
 o 60
 en
 E
   20
                   DOC
                                 o
                     DOC calculated from the
                     measured products
      creso!
          20    40    60    80    100   120
           ozonotion time [min]    IQmgOg/min-l

Fig. 1O   Ozonization  of  4-chloro-o-cresol, carbon balance
          c =  1 mmole/1;  ozone  dose 10 mg 63/min 1; pH 5.5
10 min of ozonization  (Fig.  11).   After larger additions of
ozone  (6 mg Cu/mg  C) the  solution again becomes colourless.
In this region the oxidation products formed are also better
degradable biologically.
It can be seen  from  the  example of phenols and substituted
aromatics that  some  of the  reaction mechanisms postulated for
ozonization could only be confirmed by clarifying the oxi-
dation products and  particularly by their quantitative deter-
mination.  In the light  of.the material balances obtained
the existence of primary oxidation products may also be
proved, though up to now little is known about their identity
and their properties.  Of the  remaining classes of substances
it may be said that  so far  only the reactions of ozone with
unsaturated aliphatic carboxylic acids and aliphatic alcohols

-------
                             256 -
                   2          4          6  >
                   mg ozone consumption/rug initial DOC
                     15     25        60   120
               ozonation time(min)  dose 10mg03/min-l
 Fig .  1 1   Ozonization of aniline,  biological degradability
          of the oxidation products  in dependence on the
          ozonization time
c = 1 mmole/1; ozone  dose  1O mg
                                                  1 ;  pH 6
and carbonyl compounds  have been studied in any detail.   As
regards the compounds of  other substance classes, so  far  ther<
are only indications of their possible degradability.  Even
if the determination of the oxidation products is problematic
because of the complicated composition of the ozonized sol-
utions, one property should be checked, namely the toxicity.
Little is yet known about this and more detailed work is
required.

-------
                          - 257--
 (1)   KURZMAMN1,, G.E.                  .         .. '        .
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 (2)   BREDTMANN,  M. •                  -..-.'-•.
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 (3)   SCIIAEFER, R.J.                                  '   ."   "
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      designed  by Nickhimmash
      The Soviet  Chemical Industry 6_ (1974), 10,  653

-------
                         -  258 -
(11)   HEIST, J.A.
      Ozone oxidation of waste water contaminants
      A Literature Review
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(13)   RICHARDS, D.A., FLEISCHMANN, M., EBERSOLD, L.P.
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(21)   LEBOUT, H.
      Fifty years of ozonation at Nice
      Ozone Chemistry and Technology,  Advances in Chemistry
      Series 21 (1959) , 450

-------
                         - 259 -
(22)   KATZENELSON,  E., KELTER, B., SHUVAL, H.I.
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(23)   KATZENELSON,  E., BIEDERMANN, N.
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-------
                         -* 260 T<
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 (38)  BAUMANN, H.D., LUTZ, L.R.
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      Research Technical Completion Report OWRR
      Project No. A - O37 - IDA - April (1973)

(45)  FURGASON, R.R, et al.
      Ozone treatment of kraft mill effluent
      AIChE Symposium Series70 (1974),  139, 32

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                         - 261> -
(46)  SUZUKI,  I.
     Study  on ozone treatment of .water-soluble polymers
     I.  Ozone degradation of polyethylene glycol in water
     J.  of  Applied Polymer Science 2O_ (1976), 593

(47)  MAIER,  D.,  KURZ,  R.
     Untersuchungcn zur Optimierung der Ozonanwendung
     bei der  Aufbereitung von Seewasser
     Internat.  Syrnposium Ozon und Wasser Berlin (1977), 211

(48)  SONTHEIMER,  H., WOLFEL, P., SARFEPT, E.
     Improved biological  decomposition of organic substances
     contained in biologically-cleansed sewage after ozonation
     Proceedings  3rd Symposium of the Internat. Ozone Institute
     4-6 May  Paris (1977)  ' ,   .  •

(49)  GIURGIUS,  W., COOPER, T., KARRIS, J., UNGAR,  A.
     Improved performance of activated carbon by pre-ozonation
     J.  WPCF  50 (1978), 308

(5O)  GILBERT, E.
     Uber die VJirkung von Ozon auf hochmolekulare Wasser-
     inhaltsstoffe    .    .     •  -
     To  be  published Vorn Wasser (1978)

(51)  KOPPE,  P.,  HEPKELMANN, H., SEBESTA, G.
     Die Behandlung des Abwassers  einer Zellstoff- und
     Papicrfabrik rnittels Ozon u-nd Belebtschlammverfahren
     Vom Wasser _4i5 (1976), 221

(52)  MALLEVIALLE,  J.
     Action de  1'ozone dans le degradation des composes
     phenoliques  simples  et polymerises. Application aux
     matieres humiques contenues dans les eaux
     Techniques  et sciences municipales et revue 1'eau
     70  (1975),107

(53)  MALLEVIALLE ,  LAVAL ,  Y . , ' LF.FEBRE , M . , ROUSSEAU , C .
     The degradation of humic substances in water by
     various  oxidation agents
     Proceedings  Ozone/Chlorine Dioxide Oxidation Products
     of  Organic Matter
     Cincinnati,  Ohio,  Nov. (1976), 189

(54)  GILBERT, E.
     Reactions  of ozone with organic compounds in dilute
     aqueous  solution,  identification of their oxidation
     products
     Proceedings  Ozone/Chlorine Dioxide Oxidation Products
     of  Organic Matter - ' .
     Cincinnati,  Ohio,  Nov. (1976) , 227

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                          -262 '.-..•
(55)   MEIJERS, A.P.               '   '''•-<
      Entfernuiig von organischen Wasserinhaitsstoffen,
      Farbung sowie Geschmacksstoffen durch Ozon
      Internationales Symposium Ozon uncl Wasser Berlin
      (1977J , 233

(56)   HALFON A. et al.
      Organic residue removal from waste waters by
      oxidation with ozone
      Division of Water, Air and Waste Chemistry, Am. Chem.
      Soc., Atlantic City meeting Sept. (1968),~ 32

(57)   KWIE, W.W.
      Ozone treats waste streams from polymer plant
      Water and Sewage Works _11j5 (1969) , 74

(58)   GARDINER, D.K., MONTGOMERY ,. H .A.C .
      The treatment of sewage effluent with ozone
      Water and Waste Treatment .Sept./Oct. (1968), 92

(59)   SOBER, T.W., DAGON, T,J.
      Ozonation of photographic processing wastes
      J.  WPCF 47 (1975), 2114

(6O)   WACHS, A'., NARKIS, N., SCHNEIDER, M.
      Organic matter removal from effluent by lime treatment,
      ozonat.ion and biologically extended activated carbon
      treatment
      3rd Symposium of the Internat.  Ozone Inst,  4-6 May
      Paris (1977)

(61)   HILLIS,  M.R.
      The treatment of  phenolic wastes by ozone
      Organic matter removal from effluent by lime treatment,
      ozonation and biologically extended activated carbon
      treatment
      3rd Symposium of  the Internat.  Ozone Inst.  4-6 May
      Paris (1977)

(62)   WALTER,  R.H., SHERMAN, R.M.
      Ozonation of lactic and fermentation effluent
      J.  WPCF 46 (1974), 18OO

(63)   WYNN, C.S.,  KIRK,  B.S., NcNABNEY, R.
      Pilot plant for tertiary treatment of waste water
      with ozone.  Environmental Protection Technology
      Series EPA - R2 -  73 - 146 Jan. (1973)

(64)   MALLEVIALLE,  J.
      Ozonation des substances de  type humique dans les
      eaux
      Proceedings  of the 2nd Internat. Symp.  on Ozone
      Technology,  Montreal/Canada  May (1975),  262

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                         - 263 .--
(65)  MELNYK,  P.B.,  NETZER, A.
     Reactions of ozone with chromogenic ligniris in pulp
     and paper mill waste water
     Proceedings of the 2nd Internal.  Symp. on Ozone
     Technology, Montreal/Canada May (1975), 321

(66)  NEBEL,  C. , STUBER, L.M.
     Ozone decolorization of secondary dye laden effluents
     Proceedings of the 2nd Internal.  Symp. on Ozone
     Technology, Montreal/Canada May (1975), 336

(67)  DUFORT,  J., JONES, J.P.
     Direct physicochemical treatment with ozone
     Proceedings of the 2nd Internal.  Symp. on Ozone
     Technology, Montreal/Canada May (1975), 545'

(68)  NETZER,  A., BESZEDITS, S., WILKINSON, P., MIYAMOTO, H.K.
     Treatment of dye wastes .by .o.zonation
     Proceedings of the 2nd Internal.  Symp. on Ozone
     Technology, Montreal/Canada May (1975), 359

(69)  DAVIS,  G.M. el al.      '''' -          .
     Ozonation of waste waters  from organic chemicals
     manufacture
     Proceedings o'f the 2nd Internal.  Symp. on Ozone
     Technology, Montreal/Canada May (1975) , 421 -

(7O)  SMITH,  M.A., FURGASON, R.R.
     Use of ozone in the treatment of kraft pulp mill
     liquid wastes
     Proceedings of the 2nd Internal.  Symp. on Ozone
     Technology, Montreal/Canada  May (1975), 3O9

(71)  MCCARTHY, J.M.
     The influence  of particle  size on oxidation of total,
     soluble  and particulate municipal waste water
     components by  ozone
     Proceedings"of the 2nd Internal,  Symp. on Ozone
     Technology, Montreal/Canada  May (1975) , 522

(72)  GILBERT,  E.
     Uber den Abbau von organischen Schadstoffen im Wasser
     durch Ozon
     Vom Wasser 43  (1974), 275

(73)  ROOK, J.J.
     Haloforms in drinking water
     J.  AWWA  68 (1976) , 168

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                         - 264 -
(74)  SANDER,  R. ,  KttHN, W. , SONTHEIMER', H.          '           '
     Untersuchungen zur Umsetzung von Chlor mit
     Huminsubstanzen
     Z. f. Wasser und Abwasser-Forschung 1O (1977), 155

(75)  MAIER, D., M&CKLE, H.
     Wirkung von  Chlor auf natiirliche und ozonte organische
     Wasserinhaltsstoffe
     Vora Wasser £7 (1976), 379

(76)  HUBBS, S.A.
     The oxidation of haloforms and haloform precursors
     utilizing ozone
     Proceedings  Ozone/Chlorine Dioxide, Oxidation Products
     of Organic Material, Cincinnati, Ohio, Nov. 17-19
     (1976) ,  200

(77)  LAWRENCE, J.
     The oxidation of some haloform precursors with ozone
     3rd Symp. of the Internat. Ozone In'st. 4-6 May Paris (1977)

(78)  SCHALEKAMP,  M.
     Die Erfahrungen mit Ozon in der Schweiz,  speziell hin-
     sichtlich der Veranderung von hygienisch bedenklichen
     Inhaltsstoffen
     Infcernat. Symp.  Ozon und Wasser Berlin (1977), 31

(79)  BLOCK, J.C., MORLOT, M., FOLIQUET, J.M.
     Problemes lies a 1'evolution du caractere d'oxydabilite
     de certains  corps organiques presents dans 1'eau traitee
     par I1ozone
     Techniques et Sciences Municipales. L'eau 71  J_ (1976), 29

(8O)  BUYDENS, R.
     L'ozonation  et ses repercussions sur le mode  d'epuration
     des eaux de  riviere
     La Tribune du Cebedeau 23 (197O), 286, 319

(81)  KtlHN, W., SONTHEIMER, H., STIEGLITZ, L.,  MAIER,  D.,
     Kurz, R.
     Use of ozone and chlorine in water utilities  in  the
     Federal  Republic of Germany
     J. AWWA TO (1978), 6, 326-331

(82)  NAIMIE,  G.,  AXT, G., SONTHEIMER, H.
     Zur katalytischen Beeinflussung "der Oxidation von
     organ!schen  Wasserinhaltsstoffen
     Vom Wasser 37 (1970), 98

(83)  REICHERTER,  U.F.
     Untersuchungen liber die Anwendung von Ozon bei der
     Wasser-  und  Abwasserreinigung
     Dissertation, Fakultat fiir Chemieingenieurwesen,
     Universitat  Karlsruhe  (1973)

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                           -  265 -  • .
 (84)  EICHELSDORFER, D., v. HARPE , Th.
      Einwirkung von Ozon auf Harns.toff im Hinblick
      auf die Badewasseraufbereitung
      Vom Wasser 3T (1
 C-85)  DOBINSON, F.
      Ozonisation of malonic acid in aqueous solution
      Chemistry and Industry, June 27  (1959) , 853

 (86)  BLACK,  W.T., COOK, G.A.  .
      Production of glyoxalic acid
      I.  and  EC Product Research and Development 5
      (1966) ,  4, 351                             ~

 (87)  SHAPIRO R.H. et al .  . '    \
      Ozonization products from caffeine in aqueous solution
      Proceedings Ozone/Chlorine Dioxide Oxidation Products
      of  Organic Materials,  Cincinnati, Ohio (1976), 284

 (88)  SHARIFOV, R.R., BONDAREVA , ' N . I . ,  MAMED-YARQVA, L.A.
      Oxidation von Dichlorbenzol durch Ozon
      Azerbaidzhanskii khimicheskii  zhurnal (1973) , 4,
      124 (Russ.)         :

 (89)  WALTER,  R.H., SHERMAN, R.M.
      Reduction in chemical oxygen demand of ozonated
      sugar solutions by charcoal
      J.  of Food Science 43 (1-97,8),  404

 (90)  KRAUSE,  H., HEPP, H.,  -KLUXER,  W,
      Versuche zur Zers"t6rung von Komplex- und Chelat-
      bildnern in radioaktiven Abwassern durch Oxidation
     , Kernforschungszentruin Karlsruhe KFK 287, Sept. 1964

 (91)  KUO, C.H., WEN, C.P..
      Ozonizations of formic acid, formaldehyde and methanol
      in  aqueous solutions
      AIChE Symposium Series 166, 73 (1977), 272

(91 a)  KRASNOV, B.P., PAKUL ,  D.L., KIRILOVA, T.V.
      Use of  ozone for the treatment of industrial
      waste waters
      Int, Chem. Engin. 1 4 (1974), 4, 747

 (92)  PAKUL,  D.L., KRASNOV,  B.P., SAZHINA, A.M.
      Oxidation of alcohols in. 'dilute aqueous solutions
      by  ozone
      Zhurnal Prikladnoi Khimii 47 (1974), 1, 36

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                            266  -
 (93)  KUO, P.K., CHIAN, E.S.K., CHANG, B.J.
      Identification of end products resulting from
      ozonation and chlorination of organic compounds
      cojmnonly found in water
      Environmental Science & Techn. 11 (1977), 1177

 (94)  GILBERT, E.
      Chemischc Vorgange bei der Ozonanwendung
      Int. Symp. Ozon und Wasser Berlin 77
      Colloquium Verlag Otto H. Hess, Berlin (1977), 277

 (95)  BUESCHER, C.A., RYCKMAN,  D.W.
      Reduction of foaming of ABS by ozonation
      Proceedings 16th Industrial Waste Conference,
      Purdue University, Lafayette, Indiana (1961), 251

 (96)  EVANS, F.L., RYCKMAN, D.W.
      Oijonated treatment of wastes containing ABS
      Proceedings 18th Industrial Waste Conference
      Purdue University, Lafayette, Indiana (1963), 141

 (97)  KANDZAS, P.P., MOKINA, A.A.
      Vorwendung von Ozon zur Reinigung von Abwassern von
      synthetischen oberflachenaktiven Anionen-Substanzen
      Ochistka proizvodstvennyk stochnykh vod _4 (1969), 76-86
      (Russ.)

 (98)  JOY, P., GILBERT, E., EBERLE, S.K.
      Untersuchungen iiber die Wirkung des Ozons auf Alkyl-
      benzolsulfonsaure und Waschmittel in wassriger Losung
      "Organische Verunreinigungen in der Umwelt" Hrsg,
      K.  Aurand, Berlin (1978),164
      ISBN 3-5O3-O1713-5

 (99)  REICHERT, J.
      Untersuchungen zur Eliminierung kanzerogener aromatischer
      Polyzyklen in der Trinkwasseraufbereitung unter besonderer
      Beriicksichtigung des Ozons
      gwf-Wasser/Abwasser 110 (1969), 18,  477

(1OO)  GOMELLA, C.
      Ozone practices in France
      J.  AWWA £4 (1972) , 39

(1O1)  HOFFMANN, I., EICHELSD5RFER,- D.
      Zur Ozoneinwirkung auf Pestizide der Chlorkohlen-
      wasserstoffgruppe im Wasser
      Vom Wasser 38 (1971), 198

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                         -  267 -
(1O2)  MORGELI,  B.                   .
      Entfernung von Pestiziden aus Trinkwasser
      Gas,  Wasser, Abwasser 52 (1972), 142

(103)  ROBECK,  G.G. et al.
      Effectiveness of water treatment processes in
      pesticides removal
      J.  AWWA  57 (1965) , 181

(104)  ROSS,  W.R.,  v. LEEUWEN, J., GRABOW, W.O.K.
      Studies  on disinfection and chemical oxidation with
      ozone and chlorine in water reclamation
      Proceedings  2nd Int.  Symp.  on Ozone Techn.
      Montreal, Canada (1975), 497 '

(105)  BRGWER,  G.R.
      Ozonation reactions  of selected pesticides for
      water pollution abatement
      Dissertation Abstracts B (1967), 28, 722-B

(1O6)  RICHARD,  Y., BRENER,  L.
      Oi'ganic  materials  produced  upon ozonization of water
      Proceedings  Ozone/Chlorine  Dioxide Oxidation Products
      of  Organic Materials, Cincinnati, Ohio, Nov. (1976), 169

(107)  LAPLANCHE, A. , MARTIN, G.,  RICHARD, Y.
      Etude de  la  degradation des pesticides par 1'ozone:
      cas du parathion
      La  Tribune du Cebedeau 27 (1974) , 22

(108)  LAPLANCHE, A., MARTIN, G.,  RICHARD, Y.
      Ozonation d'une eau  polluee par du parathion
      T.S.M. 1'Eau 2 (1974) , 407

(109)  DAGON, T.J.  .
      Photographic processing effluent control
      J.  of Appl.  Photogr.  Eng, £ (1978), 2, 62

(11O)  BAUCH, H., BURCHARD,  H.
      Uber  Versucne, stark  riechende oder schadliche AbwSsser
      mit Ozon  zu  verbessern
      Wasser-Luft-Betrieb J_4_ (197O), 134

(111)  CAULFIELD, M.J.
      Kinetic  studies of the ozonation of carc.inogens and
      mutagens  as  monitored by biological assay; Univ.Notre Dame,
      Dissertation Abstracts Internat.  B 38 (1978) ,  1Of 4644-4645

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                         - 268 -
(112)  SPANGGORD, R.J., McCLURG, V.J.        .,.,..    , .,
      Ozone methods and ozone chemistry of selected
      Drganics in water
      Proceedings Ozone/Chlorine Dioxide Oxidation Products
      of Organic Materials, Cincinnati, Ohio (1976), 115

(113)  SIMMON, V.F., ECKFORD, S.L., GRIFFIN, A.F.
      Ozone, methods and ozone chemistry of selected organics
      in water, 2. Mutagenic Assay
      Proceedings Ozone/Chlorine Dioxide Oxidation Products
      of Organic Materials, Cincinnati, Ohio (1976), 126

(114)  DORE, M., LANGLAIS, B., LEGUBE, B.
      Ozonation des phenols et des acides phenoxy-acetiques
      Water Research 12 (1978), 413

(115)  BAUCH, H., BURCHARD, B., ARSOVIC, M.
      Ozon als oxidatives Abbaumittel fur Phenole in
      wassrigen Losungen
      Gesundheits-Ingenieur 91 (197O), 9, 258

(116)  STRUIF, B., WEIL, L., QUENTIN, £.E.
      Verhalten herbizider Phenoxy-Alkan-Carbonsauren
      bei der Wasseraufbereitung mit Ozon
      .Z. f. Wasser- und Abwasser-Forschung 11 (1978) ,
      3, 4, 118

(117)  KOROLEV, A.A., KORENKOV, V.N., ABINDER, A.A.
      Problems of.hygienic effectiveness of ozone treatment
      of water and sewage containing nitro compounds (Russ.)
      Gigiena i sanitarja Moskva 11' (1974) ,13

(118)  KOROLEV, A.A., ABINDER, A.A., BOGDANOV, M.V.
      Hygienic and toxicologic features of products of phenol
      destruction in ozone treatment of water
      Gigiena i sanitarja Moskva J3 (1973), 6

(119)  NIEGOWSKI, S.J.
      Destruction of phenols by oxidation with ozone
      Ind. and Eng. Chem. 45 (1953), 3, 632

(120)  MATSUOKA, H. et al.
      Ozone treatment of waste water
      Report Mitsubishi Electric Co. 46 (1972),  552

(121)  ANDERSON, G.L.
      Ozonation of high levels of phenol in water
      AIChE Symp. Series Water 73 (1976), 166,  265

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                         - 269 -
(122)  PASYNKIEWICZ,  J., GROSSMAN, A.., NAWARA, S.
      Application of ozone in purification of drinking
      water containing phenols
      Przemysl Chemiczny 4^7 (1968) , 4, 231

(123)  EISENHAUER, H.E.
      Increased rate and efficiency of phenolic waste
      ozonation
      J.  WPCF _43 (1971), 2OO

(124)  EISENHAUER, H.R.
      The ozonation  of phenolic waste
      J.  WPCF 4O (1968), 1887

(1.25)  EISENHAUER, H.R.
      Dephenolization by ozonolysis
      Water Research 5_ (1971), 467

(126)  CASALINI, A.,  LEONI, A., SALVI, G.
      Ozone purification of waste water containing phenol:
      Investigations of the oxidation mechanism
      La  Rivista dei Combustibili 31 (1977) , 3, 92

(127)  GOULD, J.P., WEBER, W.J.
      Oxidation of phenols by ozone
      J.  WPCF £8_ (1976) , 47

(128)  YAMAMOTO, Y.,  NIKI, E. et al.
      Ozonation of organic compounds, II. Ozonation of
      phenol in water
      Proceedings Ozone Technology Symp. Internat. Ozone
      Inst. May-23-25 (1978),  Los Angeles, Cal.

(129)  HOIGNE, J., BADER, H.
      Ozonation of water: Selectivity and rate of oxidation
      of  solutes
      3rd Congress of the Internat. Ozone Inst. Paris, May 1977

(13O)  HOIGNE, J., BADER, H.
      Ozone initiated oxidations of solutes  in waste water:
      A reaction kinetic approach
      Prog. Wat. Techn.  1O (1978), 657

(131)  GILBERT, E.
      tlber die Wirkung von Ozon auf Maleinsaure, Fumarsaure
      und deren Oxidationsprodukte in wassriger L5sung
      Z.  Naturforsch. 32b (1977), 13O8

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                         -  270 -
,   _. .                       •          - . . i • / .. *./ . i ' i
(132)  GILBERT,  E.
      Die Ozonung  von MuconsSure
      To be published

(133)  ISHIZAKI, K.f  DOBBS,  R.A., COHEN,  J.M.
      Ozonation of hazardous and toxic organic compounds
      in aqueous solution
      Proceedings  Ozone/Chlorine Dioxide Oxidation Products
      of Organic Material,  Cincinnati, Ohio,  Nov.  (1976), 21O

(134)  GILBERT,  E.
      Investigations on the changes of biological  degradability
      of single substances  induced by ozonolysis
      Ozone Technology Symp. May 1978, Los Angeles, Cal.

(135)  GILBERT,  E., JOY, P., EBERLE, S.H.
      Ozonisierung von p-Toluolsulfonsaure und 2-Nitro-p-
      Kresol in wassriger LSsung
      To be published

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                         - 271  -

OZONE REQUIREMENT AND OXIDATION COMPETITION VALUES OF
VARIOUS TYPES OF WATER FOR THE OXIDATION OF TRACE
IMPURITIES
J. Hoigne and H. Bader
Many water constituents are oxidized in ozonization pro-
cesses.  In the last paper Gilbert showed corresponding
case studies  (1) .  The following fundamental questions thus
arise:  How complete are such oxidation reactions after a
certain ozonization time ?  Which materials are concen-
trated in the water as intermediate daughter products ?

The action of an ozonization process on the water consti-
tuents is essentially determined by two types of over-
lapping oxidation-initiating reactions  (2-5) :

-      Direct reaction of the ozone
-      Reactions of secondary oxidants formed in
       decomposition of the ozone  (OH* radical reaction) .

Following a detailed outline of the parts played by the
two types of reaction in earlier works  (see e.g.  (5)), the
rest of the paper will be devoted mainly to a method of
characterizing a water type with respect to the action of
secondary oxidants.  To this end we introduce the concepts
"oxidation competition value of a water type" and "oxida-
tion competition coefficient of a constituent" .

1.     Direct reaction of ozone
Ozone can react with many water constituents in direct
reactions :
                       Mx
                        MX +(1/1-1)03— M
                                       oxide

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                        -  272  -

Here  M  represents a specific water impurity and M  an
intermediate daughter product formed from it.  Within
the duration of the ozonization M  reacts with further
ozone molecules to form the daughter product M  ., .
Therefore, for the entire course of the reaction,  1/n
molecules of 03 are used up per converted material, i.e.
n is a yield factor, A[M]/A[0,].  The rate-determining
step of the above reactions is by definition only the
first step.  Such direct reactions of ozone are of first
order with respect to the ozone concentration and of first
order with respect to the concentration of M (4-6), and
the rate of elimination of a material M is:
            dt
                            = „
                                   _
                                   O-
                           [Ml
If the ozonization takes place in a batch-type or a plug-
type reactor, it can be inferred from the above reaction
scheme that the concentration of an individual water con-
stituent decreases with increasing time of ozonization
according to:                   __*/-r*
                r_ -i  . r. .i        l / *i
with
                Nt/[M]0 =
                      M
T/TM = TI • k0  • [03 ]
                                                      (1)
(2)
where  t^t-' ^  are tne concentrations of the water
                 constituent M at time  t  and at the
                 beginning of the reaction,
       t  is the time of ozonization,
       TM is the time constant of the reaction with respect
          to [M] , and
      k~  is the rate constant for the reaction of the ozone,
       °3

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                         -  273
t  corresponds- to the tame-of ozonization necessary in  -.
 M       c .   • • -   •                     _
the presence of an ozone concentration  [O33 for an elim-
ination of [M] to 37%  (1/e), see Fig. 1.  I/TM is a  •
reaction rate. In a description of the  disinfection pro-
cess this is often denoted by y.  If  M is an individual
substance, i.e. not a sum parameter, t  becomes independent
of the extent of the ozonization reaction, i.e. the log-
arithmic elimination curve shown in Fig. 1 becomes a
straight line.

We measured the rate constants  (k  ) for about 6O different
substances, e.g. on the basis of the rate .of  decrease  of
the O_ concentration as a function of the M concentration
 (6).  With the measurement methods so tested  the constants
for further substances are easily determined  if required.
Fig, 2 gives  some examples of these measurements.  The
results  show  that ozone is also an extremely  selective
oxidation  agent in water: the k-values  of  even chemically
 similar  materials can  vary by orders of magnitude.
            in [M]t/[M]
"1.
0
-1
-2
-3
[ Nt/[M]0
1 nn
	 ™ - I. UU
0.50
Ontj
.o/
0.10
... . o DR

	
—
(

TM


i

N,
' J ; 	 ^V •
3*T
III ^-
3 1 2 3 4 t '
ozonation time
 Fig. 1
Effect of the "direct O3 reaction" •
Logarithmic decrease of the relative concentration of
a "specified  trace impurity M, plotted against the
reaction time at a constant ozone concentration

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                          _ 274 -
            (M'W1
               to5-
               to3-
                10-
101
                        cresol
                     CH3
                        xylene
                       ) benzene
                                OH
                [fS phenol
                               aldehyde
                     >=< tetrachlorethylene
                      CH3 Hg+
                      methyl mercury
                              oxalic acid ion
                        -100
                                        -10"
-106
                         r
                         sec
Fig.  2    Left scale: Examples  of rate constants kg   (5,6)
      ""   Right scale: Time  constants T of the  reaction,
                                                            -5,
          calculated according to equation 2 for  [O 3]  = 1O~"'M
          (ca. O.5 g/m3),  n  = l.O
On  the basis of the rate  constants measured the  time  con-
stant TM for the reaction of  a constituent M can be calcu-
lated for a given ozone concentration.  A corresponding
.time scale is set out in  Fig.  2 on the right ordinate.  It
                                            — *r
refers to a mean ozone concentration of 10   mole 0,/1
             3
{ca.  0.5 g/m )  and to the assumption that the reaction
yield is r\ = 1.0.  At an  ozone concentration 10  times as
high this time scale would be compressed by a factor  of 1O,
The illustration shows that cresol enters the reaction  essen-
tially within 10 sec.  The concentration of phenol is reduced
within about 100 sec to 37% of its starting value.  (Other
compounds too,  known for  the  fact that they are  easily
chlorinated in chlorination of water, are readily degraded
by  ozone (6)).

-------
                        - 275.

In contrast to these readily oxidized substances, aldehydes,
for example, which are concentrated in sea water as inter-
mediate ozonization products, require really long ozoniza-
tion times for further oxidation  (5, 7, 8).  Acids such
as oxalic acids, which are also formed as oxidation pro-
ducts, have no chance of being mineralized further by the
direct ozone reaction, even when the ozonization is con-
tinued for hours.  Compounds such as methylmercury and
tetrachloroethylene  (.9,  1O) also hardly react by the "direct
ozone reaction".

2.     Oxidations via secondarily formed oxidants
In the ozonization of water the decomposition products of
ozone, i.e. secondarily  formed oxidants, are also available
for oxidation of any trace - impurities: depending on the
water, a part of the ozone, O.,  , is decomposed.  This part
                             o , A
increases with increasing pH: at pH 8, depending on the
water constituent, it corresponds to about half of the
available ozone within 1O min (11).  The OH" radicals
formed react very rapidly with many substances  (for liter-
ature data see ref.  3),  initiating known oxidations. The
radicals are significantly more reactive than ozone and
therefore less selective.  Correspondingly, they are rapidly
consumed by many of  the  available water constituents  (S).
Thus, the oxidation  of specific impurities is strongly
competed against by  the  presence of other constituents in
the water  (1O, 11):
                                      M o.xide
where: O.,  .   is the quantity  of  ozone  decomposed,
         •j i A
       n'     is the yield with which OH"  radicals were
              formed from decomposed ozone,

-------
                        - 276 -

        n"   is  the  yield with which OH"  radicals, consumed
            by  M, oxidize M,
        k'M,  k'^  are the rate constants with which OH"
            radicals  react with  M  or scavengers S.,
and     S^   are scavengers, including M, O-,  etc.

The elimination rate for this reaction  is:
             ,rMl     dO,  A          k '[M]
             d[M]       3,A   ,  ,„    M
               —_   	1_ . r,i.rv" .
              dt     dt
2.1.   Oxidation  competition  value of a water type (flM)
For a batch or plug-type  reactor   the relative residual
concentration of  M  at  time  t  is:
M
                  t
                                        (3)
                 AM~"TT~ " «• ** "^->^ PiJ)  w
                     KM    '  '
On the basis of equation  3, the  relative  residual  concen-
tration of M plotted on a  logarithmic  scale  against  the
quantity of decomposed ozone gives  an  elimination  curve  of
slope 1/^M  (see Fig. 3).  OM is  a normalization  value  de-
pendent on the water composition.   It  increases  with in-
creasing loading of the water with  substances  S.  that  con-
sume OH" radicals  (see ecruation  4) .  If o  does  not essen-
                                         M
tially alter with ozonization, as happens in a large number
of practical situations (cf. Section 2.4), and if M is  an-
individual compound (not a sum parameter), the elimination
curve is a straight line . (see Fig.  3).  In this  case the'
residual concentration of M is 37% ' (=  1/e)  of  the initial
value after the amount of the decomposed ozone has reached
the value n.

-------
                         - .277 -
         ln[M]t/[M]0,
                             01234 g/m3
                              ozone  decomposed ,63,4
 Fig.  3  Oxidation initiated by OH*  radicals
         Logarithmic fall of the  relative concentration of
         an individual trace impurity  M with increasing
         amount of ozone decomposed  in water.  Assumption:
         the n-value of the water is independent of ozoni-
         zation

              CH3HgOH
              101
              100
               50


               37



               25
M. 9
• 5- TO-' M
o 5-KT8 "
A 5-10'7 '

CH3HgOH
n „
"
n.
1
5
c
X
X
10 15 (g/nv>)
zone added
Fig. 4   Measured logarithmic fall of the relative con-
"~~~~~~~~  centration of methylmercury plotted against the
         amount of ozone  added (mineralization of the
         methylmercury by OH*  radical initiated reactions)
         Water loaded with  5  g/m^ of octanol. pH 1O.5;
         O.05 M phosphate (9)

-------
                        -  278 -
 We suggest the following terms' for the ft-value: '
    German :    "Oxidationskonkurrier-Wert" (des Wassers)
    English:    "Oxidations-competition value"
    French :    "valeur  de  competition a 1'oxidation"
 In principle,  it is possible to calculate ft  for a specific
 compound M and a specific water composition according to
 equation 4 on  the basis of relative rate constants well
 known for OH'  reactions (11).   However,  with such estimations
 some uncertainties regarding the yield factors n1 and n"
 always remain,  and likewise regarding insufficiently charac-
 terized water  constituents which are generally given only
 by sum parameters such as the  DOC.   It therefore seems
 better in practice to determine the ft-value of a water
 experimentally  with a suitable reference solute (12).

 In the following it is shown on some examples that the con-
 centration decreases found experimentally can be described
 by equation 3 and the ^M-value of equation 4.

 Fig.  4 shows the measured oxidation of methylmercury to
 inorganic mercury(II)  dn.ring ozonization (9) .   The water
 was  "loaded" with  a model  substance (octanol).  The observed
 oxidation of the methylmercury cannot be attributed to a
 "direct ozonization reaction"  (9)  (cf. Fig.  2),  but the
 expected logarithmic decrease  of  the  methylmercury concen-
 tration is  obtained when  the relative residual  concentra-
 tion  is  plotted  against the quantity  of  ozone  added (and
 decomposed) according  to equation 3.  As  expected on the
basis  of  equation  3,  the course of  the relative  concentra-
 tion  lines  does  not change even when  the  initial  concentra-
 tion  of  the trace  impurity is  increased by some  powers  of 1O.
This means  that  tho.  reaction is exactly of first  order
with  respect to  the  trace impurity  concentration.  In addi-
tion, the slope  of  the relative concentration lines over
the measured ozonization region remains constant.   This

-------
                        - 279 -

means that ^M does, nol; alter (significantly with ozoniza-^t ^
tion of the water. We obtained similar,'but for analytical
reasons somewhat more restricted, results for several
other model substances, as well as for trace impurities
detectable in lake water by- gas chromatography (5, 8fs 12) .

The mineralization of methylmercury can be determined
analytically particularly simply, exactly, and over a wide
dynamic range. However, methylmercury forms complexes with
many possible water constituents.  Large complex-formation
constants are already known for chloride, carbonate, phos-
phate, etc. However, since the reactivity of a compound is ,
strongly affected by such complex formation (9), for char-
acterizing a given water we recommend the use of inert
reference solutes M, such as benzene or tetrachloroethylene;

Fig. 5 shows a Q-value determination in a lake water that
is  (measured under Swiss conditions) strongly loaded. Here
0 only becomes constant after a "spontaneous ozone consump-
tion" of about O.5 g ozone/m .  The "spontaneous ozone
consumption" produces•only a parallel displacement of the
elimination lines. Even a preliminary ozonization with
1O g O_/m  has only a limited influence on the residual
slope of the elimination line (12).

(In waste water previously purified biologically the resi-
dual slope of. the curves also changes only slightly with
the degree of preliminary ozonization in the region.of.
0-10 g O«/m   (1O)).   Constant 0-values, i.e. constancy
of the residual slopes of the logarithmic elimination lines,
were observed in all the Swiss lake and ground water samples
tested by us.             ".       •   -

-------
                         - 28O -
               %M
               too
               50
                10-
                                c) .
                                tetrachloroethylene
                                      3  '  g/m3
                                   ozone added
 Fig.  5   Logarithmic decrease of the relative concentra-
 '         tion of  trace  impurities plotted  against the
          amount of  ozone added. Water  from Lac de Bret
          (DOC 4 g/m3, z C02 -^1.6-M, pH -—8.3). Compounds
          added: a)  8O mg/m^ benzene, b) 130 mg/m3 toluene,
          c) 5OO mg/m3 tetrachloroethylene  (12)
                                   10        15        20
                                     I [Sj]   octanol (g/m3)
Fig. 6   Oxidation competition- value  0M plotted against the
         DOC  model loading  OS = octanol).  pH 10.5  (O.O5 M
         phosphate).  Compounds added:  [Benzene]o = 80 mg/m3,
          [HgCH3OH]Q = 10 mg/m3,_{C2C141 =  15 mg/m3 (9)

-------
                        - 281  -   -

 The fi-values of various water -types are compared in
 Table 1, benzene and tetrachloroethylene being used as
 the reference solutes.  In water of Lake Zurich the
 ft-value for benzene at pE: 8-was about O.8 g of "ozone
 decomposed" (O   )  per m  of water. This means that O.8 g
               j r A             , .
 of decomposed ozone is sufficient to reduce the concen-
 tration of a compound such as benzene by a factor of e.
 An elimination to 5% of the initial concentration would
 necessitate 3 x n = 2.4 g of  "ozone-decomposed" per m
 (cf. Fig. 3).   At pH 10.5'the ft-value rises to 4 g/m .
 For tetrachloroethylene, which reacts exceptionally slowly
 with OH" radicals,  the corresponding values are about 5
 times as large (cf. also Section 2.3).

 Oxidation effects of similar extents were measured in
 other drinking waters (11, 12).

 Table 1   Examples of n-values for various water types
• • • • 	 	 -•• - • •-' — - • •
Lake Zuric?ib)
c)
Dubendorf
ground water
PH
7,7
10,5a)
7,6
9,0a)
3
-D- (g O3/m )
Benzene
0,6
4,0
1,0
3,0
Tetrachloroethylene
3,0
~26
6
— 12
   a) pH increased by addition of NaOH
   b) z  (C02) = 1.2 rtiM  ' ' - 'DOC ="1.2 mg/1
   c) z  (CO2) = 2.6 mM      DOC = 1.6 mg/1
2.2.    The oxidation competition coefficient
       water -constituents .  •
                                               of various
         can be treated as composite magnitudes. The contri
bution of each constituent S .  can be calculated as the pro-
duct of its concentration and a coefficient to,.  . :

-------
                        - 282 -
                  '/ '/  *M
               or:

               ft = ojjs, ] + cu0
                              Jj [Sj
An experimental example of the calculation of the  coeffi-
cients u is given in Fig. 6, which shows the measured  in-
crease of  ft   with increasing octanol content of  a model
water. We carried out similar measurements for water  con-
taining additions of bicarbonate ions, carbonate ions,
free NH_, and fulvic acids.  The linear increase of the
n-values observed with loading of a water corresponds
formally to equation 5.  The coefficients a) can be read off
from the slope of the curves.

We propose the following terms for w:
  German :    "Oxidatior.skonkurrier-Koef f izient"
  English:    "oxidaticr.-competition coefficient"
  French :    "coefficient  de competition a I1oxidation"
Table 2 gives some examples of measured coefficients co.
It can be seen that the O_.  , requirement  that decreases
                         3'A             3
the benzene value to 37% rises by 0.5 g/m  per g of octanol
loading. The other values are given in mole/mole units.
For bicarbonate the oxidation competition coefficient  is
about 1/10 as large as that for carbonate.  That is, an
elevation of the pH without elimination of carbonate leads
to a strong increase of the ozone requirement. Even the
effect of NH., (as an oxidation inhibitor) can be significant
in the case of the oxidation of trace impurities in communal
waste water pre-purified biologically if we work in the pH
region in which ammonium ions are dissociated to free NH.,.

-------
                         283 -
Table 2. .  .Examples of coefficients w for different water.
   . •_  "'   constituents  (values from ref. 12)
s
O.c.tanol
Fulvic materials"
(HCO3~ + CO?"1)
eo/ b)
NH/
NH
PH
8-10
8-9
8,0
10,5

10,5
CA
Benzene
0,5 g/g
0,2 g/g
0,1 (/mole
3,0 g/mole
0,0 g^nole
2 , 0 g/fcole
J
Tetrachloro'ethylene
2,3 g/g
0,6 g/mole
13" g/mole
0,0 g/mole
10 g/roole
       • a) Soil fraction, soluble at pH  7  and  at pH  1
       b) Corrected for the proportion  of bicarbonate

It is  remarkable that  the  coefficients  o>  for  NH3  are
higher than would have been expected  on the basis of the
low rate constants of  the  reaction of NH^ with OH*  radi-
cals  (4). The reason is that  the primary  daughter products
Mx formed from NH., very rapidly consume further oxidants,
themselves becoming oxidized  to nitrate.  Only a part of
the oxidation stages in this  process  is controlled  by  the
oxygen molecules present;  experience  shows that the con-
sumption of ozone for  the  oxidation of  NB., to nitrate  is
really large  (n1  . n"  < O.25).
 2.3.   Ratios of  the   QM~values  for different trace
       impurities (M)  in  a  water
 As  can be  seen  from equation 4,  the flM~values of a given
 water for  different reference solutes M vary in inverse
 proportion to the rate constants,  and a correction is still
 needed for different  yield  factors n".  For the ratio of

-------
                        -  284  -

the fl-value of" a reference  so-lute A-to  that  'of • reference1'•' ''"
solute B, equation 4 gives:     '            '         '  '  "i.-^*.v(:. •.

         fl-./Q., =  k' /k1  x (n "TJ/TI "B)                 (6)
          **  Jj      £3   i\      ij   xi

For the oxidations that are only initiated by secondarily
formed oxidants only the reaction of the  OH" radicals  is
significant in the systems  investigated here by us.  There-
fore, in equation 6 only the  relative reaction  rate  of the
OH" radicals need be considered.  Our earlier measurements
on other model solutions led  to a similar result (3).   The
special findings that in the  systems  tested  here the hydro-
peroxide radicals  (HOI or O_)  do not initiate oxidation of
the reference solutes was specially  checked, the OH" radi-
cals being converted into HOI  radicals  by the addition of
HjQyi in the case of benzene  and tetrachloroethylene the
H202 additions caused a positive contribution to the  £3
value, which was, however,  not yet constant.

Pig. 7 shows the ratios expected on  the basis of the con-
stants cited in the literature. The  rate  constants k ,  with
which the OH" radicals react with the corresponding  trace
impurity M are given on the left ordinate (see  ref.  3  for
literature).  The scale on  the right gives a reference
point for the expected fi -values. This  scale is calculated
with the aid of equations 3 and 4 and is  normalized  for the
case in which the water has a  a       -value of 1 g/m
                               benzene
(the waters of Lake Zurich, Lake Constance,  ground water
in Zurich, etc. have somewhat  lower normalization values
(fl .-^ O.8 g O_  /m )) .  In addition,  it  is assumed that the yield
            J , A
factor n" is in all cases the  same as  for  benzene. This
assumption is justified on  the basis  of our  present  exper-
ience, provided that only an estimation of the  ratios  is
required. From Fig. 7 it is not sufficiently clear that for
the usual organic compounds k1 values of  comparable  magni-
tude are obtained, since substances  that  display an  excep-
tionally low reactivity were  used on purpose.

-------
                        - 285 -


Consultation-, ofmore .detailed tabulated values shows,_> • ,,
however, that the rate constants k' of the majority of
organic compounds of average molecular weight are situ-
ated in a region that extends from tetrachloroethylene to

a little above benzene.
(M"W)
1010

109-


10s-




..-•CH3CH2CH2CH0,

CI-C-C-C1
	 col"
----- NHo •••• 	 •- ..,,...,.. , ..
	 'CH3COO" 	
^HC°3' 	 coo- 	 :...
COQ-
-1 n



- 10

-100

I
(go
Fig. 7.  Left-hand scale: Examples of rate constants with
                   which OH* radicals react with trace im-
                   purities  (for literature see ref. 3).
                   Note that substances with  k < 10 M"1
                   sec~l are taken to be special exceptions
         Right-hand scale: Oxidation competition value QM.
                   Scale normalized to 0,        =1.0
                   (average value found  en enefor Swiss
                   lake water, pH -~ 8} .  It is assumed that
                     M
                       = n
benzene
It follows from Pig. 7 that in a type of drinking water
expected for Swiss conditions  (provided pH <  8.5) at a
                                      3
decomposition of 1-10 g of ozone per m  a reduction of many

of the possible trace impurities by a factor  of  "e" can be

-------
                       - 286 -

reckoned with.  A reduction to 5% would require about 3
times this fl-value of ozone Ccf. Fig. 3}.

Measurements of the behaviour of the organic trace impur-
ities present in the water of Lake Zurich in concentra-
tions of only yg/m , such as toluene, xylene, chlorobenzene,
etc. show that these eliminations too correspond quantitatively
to the principles outlined here  (5,6).

2.4.   Phenomenon of constant n-values
In principle the fiM-value  is given by the slope of the
products' elimination graphs plotted on a logarithmic scale
according to equation 3.  These curves are mostly repre-
sented by a straight line for reference solutes in natural
water and even in waste water that has previously been bio-
logically purified.  In some waters a correction must how-
ever be made for an initial spontaneous ozone consumption
manifested by a straight line not passing through zero.
However, the residual slope even in these cases depends only
slightly on the preliminary ozonization, so long as this
occurs with only a small quantity of ozone which is usual in
the treatment of drinking water. The phenomenon that SI is
almost independent of ozonization (the slope of the elimin-
ation curve is constant) simplifies many estimations, but
should not be regarded as a matter of course. The fact
that £2-values frequently remain constant after a small
spontaneous ozone consumption can be explained as follows:

a)     An essential proportion of the fi-value is based on
       the contribution of bicarbonate and of the content
       of carbonate ions in the water (.11) . If the pH
       of the water does not change, this contribution
       hardly changes during the ozonization.

b)     Another essential proportion of the fi-value is based
       on the OH"-consuming action of "humic substances"
       and other refractory organic compounds, mostly of

-------
                        -  287  -

       higher molecular weight. Oxidation of these
       scavengers changes the degree of oxidation and
       perhaps also the molecular weight of these
       compounds. However, the reactivity of the whole
       organic material with respect to the OH" radicals
       is scarcely affected by this. Even water in which
       a DOC loading is simulated only by the addition
       of octanol displays a n value independent of
       ozonization. In this case the constancy of fl is
       based on the use of a DOC model compound in which
       the sum of the oxidation products formed and the
       starting substance consume the OH'radicals at
       similar rates (for a detailed discussion see
       ref.  12) .

c)     Even the effect of a substance such as NH_ changes
       only slowly with advancing ozonization: the free
       NH,. reacts really sluggidily  (4) , and, if the pH
       is considerably less than 9, the water contains
       a large reservoir of unreactive ammonium ions from
       which NH_ can always be released in accordance with
       the equilibrium conditions.

Many other water constituents, however, lose their radical
scavenger effect as the ozonization progresses.  In water
in which such constituents become decisive the ft-value
changes in accordance with the ozonization.  Such substances
seem to have only a subordinate significance in our drinking
waters.

In principle, OH* radicals can also be consumed by ozone
molecules themselves (.12) , in which case the n-value would
be expected to change with the nature of the ozone input.
The proportion of ozone in the over-all ft  decreases with
increasing instantaneous ozone concentration and increasing
cleanliness of the water. However, an experimental deter-
mination of the u)Q  coefficient may prove really difficult.

-------
                        -  288  -
                                          .    .   .     .
The substance to be measured M also gives a contribution
a>M[M] to the J2 - value.  If the sum of the oxidation pro-
ducts formed from M gives a contribution different from
the starting substance, the over-all fl-value of the water
will change with progressing ozonization. This only de-
creases in weight if M is not a true trace but a strong
impurity.  In such cases the ozonization effect is more
simply described on the basis of the ozone yield (AM/O-, . ) ,
                                                      -j f A O
as is obtained for low conversions  (start of the ozoniza-
tion) .  The kinetics of such systems have been treated in
earlier papers (9) .  The characterization of an ozonization
system with the aid of the n values  as suggested in the
present work is,  however, generally more informative and
always suitable when the water properties with respect to
indirect oxidation effects of the ozonization are to be
characterized.

In the cases investigated the same coefficients u were
found in model phosphate buffer solutions as in lake
water or ground water. When using a borate buffer (O.O5 M)
however, we observed an increase of all en-values, which at
the moment we can only explain by an alteration of the n '
value caused by the borate .

3.     Cooperation of the direct reaction of ozone and
       the radical mechanism
Since ozone has a very high substrate selectivity, very few
substances are included by chance in a region in which they
are simultaneously oxidized essentially by the ozone and
by the ozone-decomposition products.  In the treatment of
drinking water with a pH of about 8, i.e. a water in which
half of the ozone decomposes within about 1O min, this may
be just the case for xylene (cf. Fig. 2).  For a compound
such as benzene the "direct O- reaction" already plays a
part about 1OO times smaller.  On the other hand, phenol-
like trace impurities are degraded 1OO times faster directly
by ozone than the ozone can decompose at pH 8.

-------
                       - 289


There is an important difference between the two types of
reaction: the two types of oxidation lead to different
intermediate products/ i.e. different daughter products,
which can become concentrated in the water, and they are
governed by different ozonization process parameters. The
"direct 0_ reaction" can be improved by increasing the
ozone stability.  The indirect reaction, however, the "OH*
radical reaction", is based on a decomposition of ozone,and
in  contrast to the "direct 0_ reaction" it is inhibited
by constituents such as bicarbonate or carbonate.  This
property of the OH* radical reaction allows the extent of
the reaction to be ascertained experimentally, by deter-
mining the effect of the addition of relatively inert
substances such as bicarbonate (5, 11) or butanol, octanol,
etc.
 (1) GILBERT,  E.
    Chemische Umsetzungen und Reaktionsprodukte bei der
    Ozonbehandlung  von Trinkwassern
     (see paper Gilbert,  this  publication)

 (2) TAUBE, H,, BRAY,  C.
    Chain Reactions in Aqueous Solutions Containing Ozone,
    Hydrogen  Peroxide and Acid
    J. Am. Chem.  Soc.  62_ (194O),  3357-73

 (3) HOIGNE J., BADER,  H.
    The Role  of Hydroxyl Radical  Reactions
    in Ozonation  Processes in Aqueous Solutions"
    Water Res. 1O (1976),  377-386

 (4) HOIGNE, J,, BADER,  H.
    Ozonation of  Water:  Kinetics  of Oxidation .of Ammonia
    by Ozone  and  Hydroxyl Radicals
    Environm. Sci.  & Techn. Jj2_ (1978),  79-84

 (5) HOIGNE, J,, BADER,  H.
    Kinetik und Selektivitat  der  Ozonung organischer Stoffe
    in Trinkwasser                      .      .    .
    Wasser Berlin 77,  Tagung  der  Fachgruppe Wasserchemie
    in der GDCh  (Colloquium Verlag  Berlin 1978), 261-276

-------
                       - 290 -
  (6)  HOIGNE,  J.,  BADER,  H.       •       .           ,.  -, ,,
      Rate  Constants  for  Reactions  of Ozone with Organic
      Solutes  in Water
      To  be published

  (7)  SCHALEKAMP,  M.
      Die Erfahrungen mit Ozon  in der Schweiz,  speziell hin-
      sichtlich der Veranderung von hygienisch  bedenklichen
      Inhaltsstoffen
      Wasser Berlin 77, Tagung  der  Fachgruppe Wasserchemie
      in  der GDCh  (Colloquium Verlag Berlin 1978),  31-69

  (8)  ZURCHER, F., BADER,  H., HOIGNE,  J.
      To  be published

  (9)  HOIGNE,  J.,  BADER,  H.
      Ozone and Hydroxyl  Radical Initiated  Oxidations  of
      Organic  and  Organometallic Trace Impurities in Water
      175th Am. Chem.  Soc. Meeting,  Anaheim 1978, ACS  Symp.
      Series  (in press)


(10)  HGIGNE,  J., BADER, H.
     Ozone  Initiated  Oxidations of  Solutes  in  Wastewater:
     A Reaction Kinetic Approach
     Progr. Water  Technol. 1O (1978), 657-671

(11)  HOIGNE,  J., BADER, H.
     Beeinflussung der Oxidationswirkung von Ozon und
     OH"-Radikalen durch  Carbonat
     Vora Wasser 48 (1977) , 283-304

(12)  HOIGNE,  J., BADER, H.
     To be  published

-------
                        - 291 -  ,


TRANSFORMATION OF HUMIC ACIDS BY OZONE
J. Mallevialle
It is no longer necessary to stress the advantage of following
the course of humic-type natural organic materials through
the different stages of water "treatment with a particular
mention of the use of oxidizing agents.  The action of
chlorine on the humic and fulvic acids of water^ in particular
with the formation of halomethanes, has been described  in
many publications  (1, 2).  In our laboratory we are working
on the action of ozone, which is often described as being
very effective in decolorizing natural water, but we are not
confining  ourselves to strongly coloured water, since  we
believe that a large part of the organic material in water
can be compared to humic and fulvic acids - referred to as  .
"HA" hereafter  (3) .

1.       THE DECOMPLEXING ACTION OF OZONE
The structure scheme proposed by Gjessing (Fig. 1)  (4)  illus-
trates one of the  first consequences of the ozonization of
humic acids.  These acids have a "core" at the periphery of
which mineral elements may be found in addition to organic
compounds such as  pesticides. (Lindane or D.D.T.) .  When
ozonization takes  place; these various elements are released
into the water;the iron precipitates in the form of ferric
hydroxide, the manganese may be converted into MnOT ions
giving a violet tinge to the water; and the organic compounds
little attacked by ozone may be- found in the water, as  happens
for example in the case of Lindane.  We have already des-
cribed this type of phenomenon in previous publications
(5,6).

-------
                         -  292  -
            Ft       OH
       DCNZENECARDDXYLIC ACID
         OH
       METHOXy-BEH2ENECARBOXYI.li: ACID
Fig. 1  Schematic  structure of humlc acids (after Gjessing)
 2.       STUDY  OF  THE OZONIZATION BY-PRODUCTS
 2.1.     Compound-identification tests
 If one considers the example of the humic acid core structure
 put forward  by  Schnitzer and Khan  (Fig. 2)  (7), two important
 observations may be  made:
 a)        During the ozonization of natural organic  substances
 a large  quantity of by-products is formed, the number  and
 nature of which will depend on the type of the water and the
 amount of ozone used.   Detailed analysis of these by-products
 is complicated  because of their diversity and the difficulty
 of obtaining  reference compounds.  With small quantities of
 ozone we observe depolymerization with liberation of phenolic
 or quinonic "monomers";   this is what we observed,  for example/

-------
                         - 293
                                           OH
Fig. 2  Suggested structure of the -humid acid "core"
        (after Khan and Schnitzer)
 by thin-layer chromatography.  With larger quantities of
 ozone we observed the opening of benzene rings and the form-
 ation of aliphatic aldehydes and acids, as has been shown by
 many authors working on the ozonization of phenols (8,9).

 b)       Humic acids are not well-defined compounds but
 complex mixtures of organic compounds in constant evolution,
 for which it would be difficult to determine characteristics
 other than by over-all techniques.
 2.2.     Search for parameters permitting the transformation
          of the HA by ozone to be studied
 Curves 3 . and 4 represent the variation of various "parameters
 in the course of ozonization of a strongly coloured water
 free of any industrial pollution  (little-mineralized water
 containing 20-40 mg/1 of dissolved organic carbon)  (10).
 We preferred to select a real-case rather than a solution of
 HA extracted from a given- soil.  Fig. 3 is an example of the
 results we obtained, confirming' the comments in the preceding

-------
                        - 294 -
  pci/j hA
                meq/1 UV
200 H
 150n
 100-
                                   Fig.  3
                                   Elimination of humic acids
                                   as a  function of ozonization
                                   time
                     120 min
section.  The colour disappears very rapidly, while the con-
centration of COOH groups goes through a maximum and dimin-
ishes more or less rapidly.  The HA content, measured by the
reduction of phosphotungstic and phosphomolybdic acids
(polyhydroxy aromatics dosage  (11)} decreases much
more slowly than the colour.
Over-all parameters of the type of the TOG, COD,
TOD, and
2.2.1.
Fig. 4 shows the variation of several over-all parameters
currently used in this field as a function of the time of
ozonization.  Here again it is found that the colour dis-
appears very rapidly.  The COD and TOD follow approximately
the same course.  For low amounts of ozone we observed an
increase in the BOD,., which goes through a maximum before
falling off markedly.  The conclusion from this type of
results is that we have transformed the organic materials
present into more bio-degradable compounds containing more
and more oxygen without reaching total oxidation.

-------
                            - 295.
 200
 100
 /s-J
/
/

                       -.......-Color
                       	+	TOD
                       	COD
                       	TOC

     0    10    20    30   40    50   60
                            Ozonation Time (win)
100% . /*- ^, 	
95
75





50



25


/
/







/~y
/
/
/
,
1 /' /
r' /





/
/
,
/
x
/'



,

^
_/

.
. _.,

X1
X



i ^ 1
X
X

/


— — -*"





^,/
**"*

>
/
/



**"



*
/




'









Color
PolyhycJroxyaromatic
"~~ 	 " corooounas
	 ,_TOD
_.. 	 TOC




0, mg
    0    100  200   300  400  500
Fig.  4   Ozonization of  natural  waters

-------
                          - 296  -
 2.2.2.   Other parameters
 Since the determinations of BOB ' and TOB are. difficult in the
 case of low concentrations, we felt it important'to look for
 other characteristic parameters sufficiently sensitive to be
 applied in the sector of the  production of drinking water.
 We have thus been  led to use  the. absorption at 254  ran
 currently applied  in certain  countries (12),  and in parti-
 cular the fluorescence  intensity in ultra-violet light.

 The latter in fact provides two elements of characterization
 (Pig. 5), the fluorescence intensity,  which is proportional
 to the concentration, and the maximum emission wavelength
 A   which, on the  basis of our experience, is determined by
 the degree of polymerization  or size of the molecule.  The
 larger is the molecule, the more is A    displaced towards
 longer wavelengths.  For example,  Seine water upstream of
 Paris has a A   of 415  nm, while downstream of Paris its A
 is only 405 nm  (13).
                              	lionosulphonate
                               -    M.E.iei
                               ^  ticiution: 320 nm
Fig. 5  Fluorescence spectra of a solution of  lignosulphonates
        and of a solution of humic materials

-------
                        - 297 - • . ,  -


While working on a contract for the Ministry of the Environ-
ment we established significant correlations between the
fluorescence intensity and ,the various over-all parameters
described in Section 2.2.1.'- These correlations were based
on 500 water samples div.ided. into different types according
to their nature or origin."  By way of example, Table 1  shows
the values obtained for the pair of parameters "fluorescence"
and "organic carbon".  The" correlations are significant In
all cases where it is- not desired to carry out an oxidation
treatment by ozone.  This result can be easily understood if
one considers the results in Fig. 6, showing the variation
of these parameters as a function of the time of ozonization.
 60
 60
Fig. 6
Ozonization of
natural waters

-------
TABLE 1   Tests of the correlation fluorescence =  f  (TOG)
         for various waters
Correlation
Coefficient
Equation
No. of Degrees
oE Freedom
Significance
Level
All Classes
0,8 10%
< 25%
Chlorination
0,973
(F)=0,626(C)
+ 1,654
68
«,.,,
                                                                                           oo
                                                                                           I
Correlation
Coefficient
Equation
No. of Degrees
of Freedom
Significance
Level
Mo r sang
0,713
(F)=0,746(C)
+ 0,37
132
<0,1%
Viry
0,363
(F)=0,502(C)
+ 1 ,06
84
<0,1%
Suresnes
0,000
(F)=-0,01 (C)
+ 3,96
50
not
significant
Croissy
0,200
(F)=0,12(C)
+ 3,46
66
> 5%
<10%
Aubergenville
0,225
(F)=0,248(C)
+ 3,3
87
> H
<2,5%
Mare aux Evees
0,543
(F)=0,267(C)
+ 19,2
18
>0,1%
< 1 %
Various
0,417
(F)=0,637(C)
+ 0,868
61
<0,1%

-------
                          -  299  -


V: Thus/; we have just seen •. that- the' over-a 1-1 'parameters used'
  by us vary in a noticeably different manner in the course of
  an oxidation treatment.  While the TOC gives the absolute
  value of the number of atoms  of  dissolved organic carbon, it
  gives no indication of the chemical nature of the organic
  compounds present.   On the other hand, fluorescence, for
  example,  indicates the presence  of cyclic compounds without
  giving  their content in absolute value, since we have here
  a  mixture of compounds.  The  parameters we have described
  are thus in fact complementary,  and we believe it is essen-
  tial not to calculate the  elimination yields of the organic
  materials in relation to just one of these parameters,  in
  view of the risk of grave  errors.

  2.2.3.    Techniques of separation on membranes
  As has  been demonstrated by certain authors (4), natural
  waters  containing HA are complicated solutions of organic
  compounds with widely different  molecular weights.  The
  technique of ultrafiltration  through Diaflo-Amicon membranes
  makes it possible to fractionate the organic compounds
  according to different ranges  of molecular weight.  Here
  again we believe that it is necessary to use several par-
  ameters for characterizing the organic material.  (We have
  retained the TOC, absorption  at  254 nm, and fluorescence).
  The ultrafiltration technique  has also enabled us to verify
  that the HA emission maxima are  a function of their degree
  of polymerization.   Fig.  7 shows an example of this type of
  separation with a water described in Section 2.2.  After
  ozonization that eliminated 80%  of the colour, '30% of the
  fluorescence and of the 254-nm absorption,  and finally a
  certain percentage of the  organic carbon, we find an increased
  homogeneity of the distribution, explained by transformation
  of the  HA into compounds of lower molecular weight.

-------
                          - BOO -
ItPC OF
MFMBRih'E
WEIGHT
75 v.-
Mr/. -
"ov.



XM 300 c '. XM5&pM3th * PH10' PM 5,T - •„ ^-IIM 2 JIM OS
300000 SO 000 30 000 IP 000 5000 1000 SOO
. ii)


•
!
i
— _j — ^^^ 	
,
..,„
	
r*fSt*44+S
	
I*"'"!
	 i
[

-------
                         - 301 -

 water through a  5-litre "react9r. '  We -then ran comparative
 jar tests on the water with and without ozonization.  In the
 majority of cases for a  given amount of coagulant,differences
 of 5-30% were observed  in favour of pre-ozonization in the
 elimination of the absorption at 254 nm and fluorescence. In
 contrast, this improvement was only of the order of
 0.1-0.2 mg/1 in  the case of organic carbon (raw water
 3-4 mg/1).  It is probable that in a pilot or industrial
 installation the elimination of organic carbon would be
 greater due to biological degradation in the sand filter; at
 least this is what we are trying to demonstrate at the moment
 on a pilot installation  (2 m-Vh) .
TABLE 2  Effect of pre-ozonization  (1 mg  O3/l)  on  the
         efficacy of coagulation of  Seine water by
         aluminium sulphate with coagulant  aid
  NON-OZONIZED PAW WATER
A'-IOUNT OF COAGULANT
(ma/1)
riocculation eval. (15 min)
Turbiditv
(drops 0. mastic)
Ontical density at 254 nm
(referred to 1 m)
Fluorescence (v.V)
Total organic carbon
(mg/1)
0
0
48
7,52
O,64
3,3
20
2
25
5,02
O,62
2,9
40
5
16
3,72
0,57
2,3
60
5
18
3,28
0,54
2,2
80
3
23
2,76
0,49
1,9
10O
3
23
2,46
0,47
1,6
  OZONIZED RAW WATER (1 mg/1 of O.j)
AMO'JNT OF COAGULANT
(ir.g/1)
Flocculation oval. (15 irirO
Turbidity
(drops of mastic)
Ontical density at 254 nm
(referred to 1 m)
Fluorescence (niV)
Total oraanic carbon
(mg/1)
O
C
51
5,93
o,4i
3,3
20
2
25
3,42
0,37
2,7
40
3
23
2,88
0,32
2,1
60
5
2O
2,32
0,23
2 , I-
80
4
25
1 ,96
0,29
2,2
100
3
29
1 ,86
O,28
1,3

-------
                              3O2 -
 We have  also  tried to explain the  increase  in turbidity
 observed after  ozonization  by counting the  particles in  a
 Coulter  counter (Figs.  8  and 9).

 In most  cases we observed an increase in  the total particle
 count, with a slight  reduction in  the number of  large
 particles, as shown-in Fig.  9.
   kNO, Of
   nartlcles/wl
           	Raw water (T,n.p.»2 591 50O)
           	+O3 (2 pran) (T.n'.r».=78  4OO)
              T.n.p, * total number  of oarticles
    Equivalent spher
    diameters In
Fig.  8
Cholet water
        4 6 810
   t. of
   pnrticlea/ml
10
              _Raw water (T.n.p.-3 133 OOO)
              ._+03 <2 ppm) (T.n.p,«4 227 OOO)
                T.n.p, » total number of particle*
                                           Fig.  9
                                           Cholet water
  O  2   4  6 810

-------
                        - 303 -

    ' ' '**.'•, i .'< i  , -'-.'.- * i".'..'. •'•*''','„  \ <".t' i','r.'"-. '. ,:"  "'.. ',' ",'r  '", '.''•'   ••'.' ";   ,
3.2.     Effect of ozonization on filtration on granular
         carbon                                 .
Fig. 1O shows the flow diagram of a waterworks  for treating
Seine water upstream of Paris.  Three industrial lines  and
a pilot line provide a comparison of the  efficacy of
different combinations of ozonization and filtration  in a
refining treatment.  We shall not list  here the results on
the elimination of micropollutants, since this  has already
been done and will be dealt with in various publications  (14).
We have simply indicated by way of example the  variation in
the fluorescence/organic carbon  (=F/C)  ratio.   It is  noted
that after each ozonization operation F/C diminishes  markedly
and increases again after filtration through granular carbon,
which confirms the results we obtained  in the first part of
this study.

3.3.     Biological degradation of organic materials
Fig. 11 is a flow diagram of a waterworks for the treatment
of Seine water downstream of Paris.  The  two parallel
industrial lines (continuous lines) are based on two  diff-
erent principles,  one  (Chabal type) using slow filtration,
the other using standard clarification  treatment with the
addition of powdered active carbon.  Over several months of
operation these two lines give equivalent results on  the
basis of the organic carbon as a parameter.  On the other
hand,  the absorption at 254 nm and in particular the  fluor-
escence are much greater at the outlet  of the slow filtration
line.

Furthermore, the maximum emission wavelength of fluorescence
is greater in this last line.  Two explanations are possible:
the slow filtration has a smaller eliminating- effect  on
humic acids of high molecular weight, or  the phenomenon is
caused by the formation of metabolites  of relatively  high
molecular weight by microorganisms.  This is what we  intend
to study during the coming months.

-------
                           -  3O4 - v



 Fig. 11 also shows, by  means  of the broken lines, an  experi-

 mental line  in which  preliminary oxonization is carried out
 before the slow filtration  stage.   The results obtained
 should enable  us to confirm the increased bio-degradability
 of organic materials  after  oxidation  with ozone.
                                Raw Water
                                         Decantation (5)
 Deeantation + Active Carbon Decantation (4)
 (3)         Powder
    Sand Filtration (6)    Sand Filtration (7)   Sand .Filtration
  F/C:
  0,36
               Ozonization
                (9)
      Ozonization(ll)  Granular Carbon  Granular Carbon Granular Carbon
                   Filtration  (12)  Filtration (13) Filtration (15)
F/C:
0,20

F/C:
0,21
/
*'
Ozonization
(Pilot)l (14)


F
F/C:
0,12
F/C
0,16

F/C:
0,80


    Industrial Line   Industrial Line  Industrial Line  Pilot Line
        NO-1           No.2           No.3         No.4
Fig.  10  Morsang/Seine Waterworks
          Flow diagram of different  lines  of treatment

-------
                                      Raw water
       Pre-
       chlorination
  Coagulation  flocculation
  with  adjuvant  and addi-
  tion  of  active carbon
  powder
r        _  •*          f
I Coagulation floccu-.  |
I lation  with coagulantj
! aid                   i
       Rapid  sand
       filtration
r	JL___,
I   Ranid  sand     ;
I                 '
   filtration     |
                                  L. „. ^, _
 F  = 0,75
TOC = l,'l
                          I Ozonization'
            V
                                  'Slow  filtration
                                  1 _________ i
Bactericidal treatment
with chlorine peroxide
                                                           roughing tank
                                                               (gravel)
                                                           Addition  of
                                                           active  carbon
                                                           powder
                                                           First sand
                                                           filtrati'on
                                                            Slow
                                                            filtration
                                                                   R  =1
                                                                  TOC = 1,6
                                                    o
                                                    ui
                                                       Bactericidal  treatment
                                                       with chlorine peroxide
Fig.  11

-------
                        - 3O6 -  -

4.       CONCLUSION
Several observations may be made about the various tests
carried out:

-        It is practically impossible to achieve total
degradation of natural humic-type organic materials by means
of ozone.  In the majority of cases there is a conversion of
these organic materials into compounds of lower molecular
weight.  A practical conclusion may thus be drawn:  The time
of contact and the amount of ozone must not be reduced more
than it is necessary,  and it will often be advisable to
follow the ozonization with a filtration treatment, e.g. on
granular active carbon.

-        When the different behaviour of various waters with
respect to oxidizing agents such as ozone is considered, it
must be realized that each water constitutes a special case
and that a study of the ozonization by-products will be very
difficult in view of their extreme diversity.

-        When carrying out oxidation treatments it seems
important to us to have available a selection of parameters
of the type described.  Elimination yields calculated just on a
single parameter will.in most cases lead to wrong conclusions.

-------
                        - 307 -
 (1)  ROOK,  J.J.
     Formation of haloforms during chlorination of natural
     waters        •                   ...••,.;.•
     Water  Treatm. Examin.  2_3_ (1974), 234

 (2)  STEVENS,  A.A.,  SEEGLER, D.R., SLOCUM, C.J.
     Products  of  chlorine dioxide. Treatment of organic
     .materials in water
    _I.O,I. Workshop Ozone/Chlorine Dioxide Oxidation Products
     of 'Organic Materials'.  17-19 Nov. 1976, Cincinnati, USA

 (3)  SONTHEIMFR,  H.      '                    ......
     "Conference at Karlsruhe, Federal Republic of Germany
     (1,975)

 (4)  GJESSING,E.T.           '                             •
     Physical  and chemical  characteristics of Aquatic Humus
     Ann Arbor Sciences (1976)               '

 (5)  BUFFLE, J.Ph.,  MALLEVIALLE, J.
    .Le role des  matieres humiques envisagees comme agent
     d1 accumulation et vehicule" des" substances toxiques dans
     les eaux.'      .           •          ,
     Techniques et Sciences Municipales,  n°special juin(1974),
     331-340           '  •       '  '             ••

 (6)  MALLEVIALLE, J.
     Degradation  of humic substance in water by different
     oxidation agents (ozone, chlorine,  chlorine dioxide)
     I.O.I.. Workshop Ozone/Chlorine Dioxide Oxidation Products
     of Organic Materials,  17-19 Nov.; 1976, Cincinnati, USA

 (7)  SCHNITZER, M,,  KHAN, S.U.
     Humic  substances in the environment
    'Marcel Dekker,  New York (1972)  ~           . .  . .

 (8)  EISENHAVER,  H.R.
     The ozonation of phenolic  wastes
     J. WCPCF  4O  (1968), 1887

 (9)  DORE,  M.
     Ozonation des phenols  et des acides  phenoxy-aeetiques
     Water  Research 12 (1978),  6,

(1O)  MALLEVIALLE, J.
     Les agents complexants naturels des  eaux
     Etude  des proprietes physico-chimiques des matieres
     humiques  et  de  leur transformation par ozonation
     These  presentee a 1'Universite Paul  Sabatier de Toulouse
     (Nov.  1974)

-------
                        -  3O8  -
(11)  -
     Standard Methods for the Examination of Water and
     Waste Water
     13 Ed.,  346             ' "

(12)  SONTHEIMER, H,  et al.
     The Miilheim Process
     J. AWWA  TO (1978) ,  1, 393-396

(13)  MALLEVIALLE,  J.
     Etude de la signification de la fluorescence dans les
     eaux dans ses rapports avec leurs qualites executee
     pour le  Ministers dfe 1'Environnement (Avril 1978)

(14)  RICHARD, Y.,  FIESSINGER, F.
     Emploi complementaire' des traitements ozone et charbon
     actif
     3©me Congres  International de 1'Institut International
     de I1Ozone, 4-6 Mai 1977, Paris

-------
    OZONE AND HALOGENATED ORGANIC COMPOUNDS

    E.  de Greef,  J.  Hrubec and J.C.  Morris .
1.   Introduction                           .  .

    Advances in the analytical determination o± "organic
    pounds in water and the discovery of the formation of
    halomethanes during chlorination are the most important
    factors leading to the initiation of a number of studies
    on the influence'of ozoriation on halogenated substances
    in drinking water. During the initial period, after the
    finding of the formation of trihalomethanes, special
    attention was paid to the effects of ozone, which was
    proposed as one of the most hopeful alternative oxidants
    to chlorine, and to the ramoval of halomethane precur-
    sors, as well as to the removal of halomethanes them-
    selves. During a more recent period interest has been
    focused  more upon the general influence of ozonation
    on the presence of halogenated compounds.

    In the following paper the influence of ozonation on the
    occurrence of halogenated organic compounds is discussed.
    Special attention is paid to the combination of ozonation
    with breakpoint chlorination in relation to observed chan-
    ges in the concentrations of haloraethanes. In addition to
    this/ possible mechanisms for observed phenomena are pro-
    posed.

-------
                           - 310 -

                                    • - >               '     '  ' Y *
2.  Formation of Halomethanes by Chlorination Following
    Pre-ozonation

    The influence of preoxidation by ozone on the formation
    of' halomethanes has been studied intensively by Rook  (1,2)
    and Montiel  (3). These  studies have been carried out  un-  •
    der conditions  similar  to, those  in practice with regard
    to the dose  of  ozone, pH and  temperature of the treated
    water.

    The use of preozonation to reduce the formation of halo-
    methanes was based on the assumption that ozone, being
    the stronger oxidant( would destroy or oxidize those sites
    in the organic  molecule which are suited for the forma-
    tion of.halomethanes  (2) .

    From- Table  1 it can be seen  that pre-ozonation as a
    part of the, water treatment at two Rotterdam Water-Works.
    and the Paris Municipal Water-Works at Orly has no sub-
    stantial effect on the  reduction of halomethanes when
    the water is subsequently chlorinated.

    Also, according to the  results of studies by the E.P.A.
    (6) ozone at a  dosage level less than 5 mg/1 will not
    decrease the concentration of trihalomethane'precursors.

    The described findings  are only  valid for the application
    of pre-ozonation without subsequent adsorption on acti-
    vated carbon and without additional biological processes
    like slow sand-filtration  (7). Also the combination of
    ozonation with  ultra-violet radiation does not apply  be-
    cause in these  combinations the  application of U".V. seems
    to provide  a positive contribution to the increased re-
    moval of both organic matter  together with halomethane
    precursors  (6,9).

-------
                           - 311 -
   TABLE 1   Formation of halomethanes (pg/1) by use of ozone
            prior to chlorination (lit.  2,3)         '''

' Contact time
after ozonation
(in hours)

0.15
4-5

24
without ozone
Water treatment plants , - ' ,•>-,•
Orly
CHC13

Rotterdam-Kralingen
CHC13'

I
2O
30
22
21

50
-
3O
32
~~__~^__
CHCl2Br


8
11

11 .
18
CHClBr,


8
9

7
11
Rotterdam-Berenplaat
CHC1,
,
CHCl2Br


4 2.3
7

12
12.5
2.5

5.5
5.8
CHClBr2
.-••' • : '. •

2.5
2

• 3
2.5
    Furthermore, it should be noted that also some labora-
    tory studies have indicated that, when the ozonation is
    used prior to"chlorination, the ozone did remove precur-
    sors and consequently reduce'the halomethane content to
    some extent' (4,5,6).                        ' :  -  •• •

    It seems that only additional studies directed to,-im-
    proving our knowledge of the chemistry of ozonation re-
    actions with organic substances, particularly the-mecha-,
    nisms and kinetics involved/ can provide a better, insight
    into the problem, of preozonation and halomethane forma-
    tion.
3.   Removal of Halomethanes by Ozonation
    The tests which have been carried out to study,the effects
    of ozonation on the removal of halomethanes .(3,4) have
    shown that ozone,  under the conditions of water,treatment
    practice and at the level of ozone doses concerned, does..

-------
                           - 312 -

    not'decrease the cbhcehtrati'ons :df" "haiome-thanes already'"
    formed prior to ozonation. Only with unrealisticially
    high doses of ozone does removal of halomethanes seem to
    occur and even then the removal may be a result of vola-
    tilization (9).
4.  Influence of Ozonation on the Behaviour and Formation
    of Halogenated Organic Compounds-

    The most extensive data with regard to the influence of
    ozonation on halogenated organic compounds have been
    published by Stieglitz et al. (1O). These data are based
    on the measurement of chlorinated organic compounds at
    several German water treatment plants which use ozone
    and are situated along the river Rhine. Results on the
    oceuriEnce of halogenated organic compounds were obtained
    by means of determinations of dissolved organic chlorine
    (DOC1), non-polar dissolved organic chlorine  (DOC1N) and
    gaschromatographically detectable organic chlorine  (GOC1),
    and further by means of identification of different ha-
    logenated compounds by GC-MS. A*s can  be seen, 'Table 2  shows
    a substantial increase in DOCl-N as well as the gaschro-
    matographable organic chlorine after  ozonation of  a
    bankfiltered water. Furthermore, like chloroform,  tetra-
    chloromethane, trichlorpethylene,  tetrachloroethylene,
    tetra-  and hexachlorobutadiene have increased in measured
    concentrations as a result of ozonation.

-------
                       - 313 -
   TABLE 2  Organic chlorine within different•treatment steps
            for a Rhine water utility using ozonation (8)




Sample
Rhine water
Sandbank Filtrate
Ozonated Sandbank
Filtrate


Dissolved'
Organic.
Chlorine
99
61

' 45
Chlorine
pg/i
Dissolved
. Organic
Chlorine
Nonpolar
15
4

,0

Gas chroma tographi-1-
• cally
Detectable
Organic Chlorine
9.5
4.8 '
• • .
	 	 8-3 	 i
On the other hand,a decrease in total DOC1 as a result of
ozonation is evident.

Another publication of Stieglitz  (1O)  (figure 1) gives
the changes of organic chlorine concentrations in water
after different treatment steps. This study demonstrates
a clear increase in GOC1 and "aliphatic chlorine and de-
crease in aromatic chlorine following ozonation.

-------
                       _  314  -
5.
Ul
Z
a
S
o
o
X
z
S
^•
o
z
o
u
   3_
   2.
         • GOCL
         * AROMATIC Ct
         C! ALIPHATIC CL
      RIVEB  I
      WATER  *
  T	1	1	
     AFTiR  I
     OZON  *
 BANK    DRINKING
FILTRATE   WATER
                     Fig.  1
                     Concentration  of  organic
                     chloride after different
                     treatment processes  (13)
According to the authors of these publications the in-
crease  in certain chlorinated organic compounds following
ozonation can be explained by a breakdown of chlorinated
organic compounds with high molecular weights to mole-
cules which can be detected and identified by available .  .
analytical techniques.

A study similar to those of Stieglitz et al. and Ktihn et al
has  been carried dut by the authors at the National Insti-
ture for Water Supply in the Netherlands.

In this study the effect of ozonation under practical
conditions in two treatment plants was observed.
 The source of water in both cases is the river Rhine. The
 difference in treatment, however, is that the first plant
 uses bank filtration,  in the second one the water is
 stored in an open reservoir with a mean residence time
 of  150 days. Before ozonation break point chlorination^
 coagulation and filtration are applied.

-------
                           315 -
  The objective of the study was to determine and measure
  quantitatively as far as possible the individual organic
  compounds.  This was done according to the general lay-
  out shown in Figure 2.
GC - HC -
IDENTIFICATION


(
Fig. 2  Layout of the analyses of individual organic
        compounds and bromide
  Details concerning the applied analytical procedures
  can be found in the annex.

  In Table  3  the results of the" analyses  in relation to
  the first water treatment plant with bank filtration  are
  tabulated.

-------
                       - 316 -
  TABLE 3  Organic compounds before and after ozonation
           in a water treatment plant using Rhine river
           water after bank filtration
Compound

chloroform
dibromoch lor ome thane
bis (2-chloro-isopropyl) ether
1 , 2-dichlorobenzene
1 , 4-dichlorobenzene
1 , 3-dichlorobenzene
trichlorobenzenes
trichloroethene
tetrachloroethene
benzene
. dibuthylphtahalate
i_« _...i —
Concentratic
Before ozone
:
2500
500
200
200
not present
'
30
30
300
Dn (ng/1)
After ozone
100
100
3000
500
200
200
not present
3OO
100
10
3000
A striking feature in these data is the relatively high
amount of bis(2-chloro-isopropyl)ether which is, as to
be expected, not broken down by ozonation.

Furthermore, the observed increases of tetrachloroethene
and trichloroethene should be noted. These increases have
also been found by Stieglitz.

The di-butylphtalate is believed to be an artifact, ori-
ginating from plastic tubes that are used in the treat-
ment plant.
The situation in the second water treatment plant is
shown in Table 4.

-------
                     - 31
TABLE 4   Organic compounds  before and after ozonation
          in a water treatment plant using Rhine water
          after open storage and chlorination
Compound



chloroform
dich lor obromome thane
ch lor odibromome thane
dich lor oiodome thane
bromoform
tetrachloroethene
bromochloroiodomethane (?)
ch lor o toluene
hexachlorobutadiene
bis (2-chloro-isopropyl) ether
1 , 2-dichlorobenzene
1 , 4-dichlorobenzene
1 , 3-dichlorobenzene
1,2, 3-trichlorobenzene
1,2, 4-trichlorobenzene
heptanol
ethylbenzene
alcohol or ketone
alcohol or ketone
alcohol or ketone
Concentratic
Before ozone


10.000
1 3 . 000
3 . 000
-
300
300
-
30
100
500
1OO
400
200
. 100
10O
-
30
30
300
10O
Dn (ng/1)
After ozone


10.000
12.500
1 0 . 000
1 .000
3.000
1 .000
300
TO
10O
-
1OO
200
200
1OO
10O
30
10
30O
100O
300

-------
                         318  -
From the results of the analyses it can be ,noted ,that the
use of breakpoint chlorlnation prior to ozonation has pro-
duced much greater concentrations of halogenated organic
compounds than those found in the bank-filtered water..

As expected/ most of the halo:genated compounds are not or
only slightly removed by subsequent treatment with ozone.

Since chloroform and dichlorobromomethane did not Increase
during ozonation, it is not likely that the rather drastic
increases of chlorodibromomethane and bromoform are due to
a prolonged action of the breakpoint chlorination. There-
fore another mechanism must be involved.

What really strikes us from these results are the increased
levels of brominated and possibly iodinated haloform com-
pounds found after ozonation. Possible explanations for
this phenomenon are discussed in section 5.

The oxidation of bromide to the already mentioned hypo-
bromous acid is confirmed in Table 5.
TABLE 5  Results of the analyses of bromide
 chlorination before 0
              after
 without C12  before O
              after  O

33
•H
33
33
Cl (mg/1)
234
237
177
178
Br (ug/1)
440
"
190
410
2OO

-------
                          - 319 -

                                      i      ,    . - >   * ~    -1
       • - .    - " • 5 ^ C . f '<  " " *   " ' '„ _' 7 "  . ' , - ,  ', .      • *  - • > > •  - - -  > - -
    The bromide-ion concentration prior to ozonation was about
    the same in both cases (4OO yg/1)  and in both cases a 5O %
    reduction occurred as a result of ozonation. .
5.  General Considerations on the Mechanisms of Halogenation
    During Ozonation

    The observed increases in haloforms and other halogenated
    compounds after ozonization are generally not to be con-
    sidered as a result of halogenation, except for the bro-
    minated and iodinated compounds. Rather it must be regar-
    ded as an outgrowth of the incomplete analytical techniques
    currently available. There is no evidence that increased
    chlorination has occurred; rather it appears that non-ob-
    served chlorinated compounds have been rendered accessible
    to observation.

    The increase in brominated and iodinated organic materials,
    particularly the halomethanes, is to be attributed directly
    to ozonation. Rook  (1) has demonstrated formation of HOBr
    front Br  in ozonation of Rhine water and has shown an in-
    creased bromine content in halomethanes formed on chlori-
    nation after ozonation. Determinations in the present study
    showed a decrease in Br  content from O.4 mg/1 to O.2 mg/1
    as a result of ozonation. Concurrently with this decrease,
    the increases in CHBr^Cl from 3 to 1O yg/1 and in CHBr-,
    from O.3 to 3 yg/1 in ozonation of a sample ( Table  3)
    are noteworthy. Another possible explanation for these
    observations, advanced by Stieglitz et al., is that ozo-
    nation splits non-volatile chlorinated organic compounds
    into smaller volatile fragments that retain the organic
    chlorine originally present. This proposal is attractive
    in a general way, but is difficult to adapt specifically
    to substances like trichloroethylene and tetrachloroethy-
    lene without postulating some unlikely starting materials.

-------
                       - 320 T-.

A similar but even .more speculative proposal jan'be ad-
vanced with regard to changes in polarity of halogenated
compounds rather than changes in molecular size, as the
properties producing incomplete measurements of organic
chlorine already in water before ozonation.

Chlorinated lower organic acids and amines are examples
of materials that might be responsible for this pheno-
menon. The increase in extractable organic material at
the expense of adsorbable dissolved organic chlorine ob-
served by Stieglitz implies an elimination of water-solu-
bilizing groups like OH, COOH and C=0. Once again, how-
ever, the increase in heavily chlorinated ethenes is not
well explained.

A different type of explanation can be visualized if it
is postulated that organic  (or other) material in the
original water can complex or otherwise interact with vo-
latile chlorinated- compounds to decrease their activity
and thus make them less prone to volatilization or extrac-
tion. Then, when the complexing or restraining materials
are broken up or made ineffective by ozonation, the chlo-
rinated materials are released at full activity to give
a greater response in analysis.

The necessary initial assumption may seem improbable, but
so little is known of phenomena at the submicrogram per
litre level that it ought not to be summarily eliminated.
If it is valid, then the observed results with polychlori-
nated ethylenes follow almost automatically.

Finally, it may be noted that, if at any point in the com-
plex sequences of reactions that may follow an initial
ozonation step there occurs a nucleophilic displacement
reaction with transitory formation of carbonium ions> then
the high concentration of chloride in Rhine water provides

-------
                       - 321  rK V  '.  -*

an ".excellent .©pportunity for- nueleophilic displacement"
by chloride rather than the normal reagent. In this event
an increase in carbon-chlorine bonds would occur without
intermediate occurrence of elemental chlorine or HOC1.

As an addendum to this mechanism it may be noted that dis-
placement of the carbonbromine bond is normally much
easier than displacement of a carbon-chlorine linkage.
It is thus conceivable that, following -the formation of
tribromomethane according to the first mechanism, the •
reaction

              Cl~ + CHBr3 	»  CHBr2Cl + Br~

occurs subsequently by nueleophilic displacement.
The possible mechanisms are once again summarized in  -
Table 6.
 TABLE 6   Possible mechanisms  for observed increases in
           halogenated  compounds  on ozonation
  1. O3 + Br   (I~) 	> HOBr 	>  Br derivatives  (I-deriv.)
     (Note: Br(.4 mg/1  before) 	> Br~  (.2  mg/1  after)
  2. O-, splits non-volatile chlorinated  compounds  into
    smaller volatile  chlorinated  fragments
  3. O-j attacks polar  spots in molecules to eliminate non-
    polar  (adsorbable)  chlorinated fragments
  4. Ozonation breaks  up complexing or adsorbing materials
     (fats, humates) that inhibit  volatilization or
     extraction
  _>. Occurrence of any  SN1 nueleophilic  processes  in ozo-
    nation reactions would allow  Cl  substitution

-------
                           -  322  -- '    •

6.   Conclusions

    From the results of this study and previous ones it is
    clear that the use of ozone during the preparation of
    drinking water can also result in the formation of halo-
    genated compounds. Our study of the two water treatment
    plants in the Netherlands shows that such a production,
    particularly of brominated compounds/ is enhanced in case
    chlorination precedes ozonation. The mechanism for this
    phenomenon probably involves intermediate production of
    bromine, but should be studied further.

    A small-scale pilot plant, suitable for studying the
    mechanisms of reactions of oxidants in different types
    of water is being constructed at the Dordrecht facili-
    ties of the N.I.W.S.

    The effects of individual oxidants, including U.V.-ra-
    diation as well as different combinations of them will
    be evaluated by identifying the products formed and by
    testing the mutagenicity of the oxidized water using dif-
    ferent types of micro-biological screening tests.

-------
                       -  323  - .
ANNEX 1
Experimental

In order to measure halogenated compounds as completely
as possible, four different.concentration and separation
techniques  (head-space analysis, Grob stripping, Junk
XAD-resin adsorption and cyclohexane extraction) were
used on portions of the same samples. Subsequent analyses
were carried out with specialized GC instruments and co-
lumns and also with GC-MS apparatus. In addition, bromide
analyses were performed according to the Fishman-Skougstad
method  (11) as modified by Rook  (2).

Head-space analyses were used for determination of very
volatile substances, such as chloroform and trichlorethene.
Sample portions of 0.4 1 were mixed with- 0.1 1 N~ in 5OO ml
closed vessels for 1 hour at 30 C. Head-space samples, of
10, 100 and 1000 yl were then injected into the inlet of
a TRACOR - 550 gaschromatograph with a 5O m capillary glass
column coated with UCON and with a   Ni-EC detector.

Extraction with cyclohexane-ether was used principally for
estimation of chlorinated aromatic compounds and haloforms
with elimination of background interference of non-extrac-
table substances. One litre portions of sample were ex-
tracted with equal volumes of a  1:1 cyclohexane-ether mix-
ture. After the immiscible liquids had been shaken to-
gether vigorously in a separatory funnel, the liquid layers
were allowed to separate and then portions of the upper
organic layer, without concentration were injected at the
inlet of a VARIAN-180O/20OO gaschromatograph having a ca-
pillary column with 0V 1 equipped with a Grob-splitter with
double parallel detectors,  1 electron capture and flame
ionization  (12).

-------
                       -  324.--
Grob stripping  (1.3, 14) was carried out on 5 litre por-
tions of sample that were maintained at 30°C in a closed-
loop apparatus through which N~ was recirculated for 2
hours at 2 litres per minute.
Vapt>rized organic compounds were collected on 1O - 2O yg
of carbon powder. After stripping had been completed, ad-
sorbed materials were eluted from the carbon with three
successive 8 ml.portions of CS_. .The eluates were com-
bined to give a final total volume of about 20 ,yl, which
was stored at -40 C until analyzed.

For analysis, a 1 jil portion of the eluate was. injected
into the part of a CARLO-ERBA 2101 gaschromatograph con-
taining a 50 m glass capillary column coated with UV101;
it was equipped with a flame-ionization detector.

For a more complete survey of higher-boiling and more po-
lar compounds, concentration on XAD resins was performed
according to Junk (15, 16). Fifty litres of a sample
were passed upflow through 3O ml beds of 1:1 mixed XAD-4
and XAD-8 resin "(Serva, Heidelberg) held in a 15 mm glass
tube. The resins had previously been cleaned by repeated
batch extraction with ether, ethyl acetate and ethanol;
they were stored under ethanol. After the adsorption step
was finished, the resins were extracted with successive
portions of ether to a total volume of about 3O ml, which
was subsequently reduced to 5OO jil by evaporation at 0 C
into a pure N~ stream. One pi portions of this concentrate
were analyzed with the same gaschromatographic equipment
described in the section on the Grob method.

GC-MS analyses of the Grob and Junk concentrates were per-
formed with a VARIAN gaschromatograph containing a 5O m,
UV-101 coated, glass capillary column. The chromatograph

-------
                      -. 325 -
was programmed"for 5'minutes at O ; -then 3 minutes at
3O , followed by continuous increase  at 4  per minute
from 3O C to 18O C. The exit was connected to a Finnigan
quadrupole mass-spectrometer with one second scar-time.
fhis was coupled to a W.D.V. data system. Spectra were
compared with the "Eight Peak Index"  or, by telephone
hook-upfwith the U.S.  E.P.A. data bank.

Bromide determinations were based on  the catalysis by
Br , or iodide oxidation by permanganate (11).  After
5 minutes of reaction, the-I2 formed  was extracted with
tetrachloroethane and measured spectrophotometrically.
(1) ROOK, J.J.
    J. AWWA 68 (1976) , 168
(2) ROOK, J.J.
    Het ontstaan van trxhalomethanes bij behandeling  van
    drinkwater met chloor
    Dissertation L.H. Wageningen  (1978)
(3) MONTIEL, D.
    Les halomethanes dans 1'eau. Mecanismes de  formation,
    evolution, elimination
    Dissertation 1'Universite P. et M. Curie, Paris  (1978)
(4) CHIAN, R.S.K.
    Identification of End Products from Ozonisation of
    Compounds Commonly Found in -Water
    AWWA, Research Foundation, Water Reuse Report 8
    (1976) ,13
(5) -      '
    EinfluB der Ozonisierung auf organische Wasser-
    inhaltsstoffe
    Report F.J. Borrus & Cie., Boncourt  (1976)
(6) SYMONS, J.M. et al..  '   -'
    Ozone, chlorine, dioxide and chloramines  as alternatives
    to chlorine for disinfection of drinking  water
    E.P.A. Report  (1977) "

-------
                        -  326  -
 (7)  EBERHARDT, M. et'al.
     gwf-Wasser/Abwasser 116 (1975), 6, 245-247

 (8)  KOHN, W. et al.
     J. AWWA 70 (1978), 326

 (9)  SYMONS, J.M.
     Interim Treatment Guide for the Control of Chloroform
     and Other Trihalomethanes in Drinking Water
     E.P.A. Report  (1976)

(10)  STIEGLITZ, L. et al. »
     Vom Wasser 47  (1977), 347

(11)  FISHMAN, M.J., SKOUGSTAD, M.W.
     Anal. Chem. 35 (1963), 146
(12)  PIET,  G.J.  et al.
     A fast quantitative analysis of a wide variety of
     halogenated compounds in surface-, drinking- and
     groundwater
     Internat.  Symp.  on the Analysis of Hydrocarbons and
     Halogenated Hydrocarbons in the Aquatic Environment,
     Toronto, May 1978          '  '                     •  •

(13)  GROB,  K. et al.
     J.  of  Chroin 106  (1 975) ,  299-315

(14)  GROB,  K. et al.
     J.  of  Chroin. 117 (1976) , 285

(15)  JUNK,  G.A.  et al.
     J.  of  Chrom. 99  (1974),  745

(16)  JUNK,  G.A.  et al.
     Z.  Anal. Chem. 282  (1976),  331

-------
                           -. 3.27 -
NOTE ON THE HALOFORM FORMATION POTENTIAL OF PRE-OZONIZED
WATER                             '    -'    .   -
J. Hoigne
The papers given at the conference about the effect of a
preliminary ozonization on the subsequent chlorination
processes show clearly that the action of ozone can depend on
a wide variety of reaction and water parameters.  This corres-
ponds fully to what we have come to expect in the light of the
present-day knowledge of the kinetics of ozonization processes.
We should like to propose, the following hypothesis for dis-
cussion: depending on the performance of the ozonization, the
water constituents are predominantly oxidized by direct
ozonolysis or by secondarily formed OH" radicals  [1].  In the
example of the oxidation of an aromatic ring system the two
types of reaction lead to different intermediate products
(Fig. 1).  Ring cleavage is mainly expected in ozonolysis,
with the formation of dicarboxylic acids, such as oxalic acid,
in subsequent steps.  In these cases enrichment of trihaloform
precursors in the water need hardly be reckoned with. However,
the proportion of ozone that decomposes in water to OH"
radicals oxidizes aromatic ring systems via hydroxylation to
hydroxycyclohexadienyl radicals, which,give hydroxy-substituted
benzenes (phenols, resorcinols etc.).  Compounds of this kind
are known to be particularly reactive trihaloform precursors.
If the ozone reaches the reaction centre before it decomposes,
these compounds are rapidly oxidized further by ozone.  In
the opposite case, it is to be expected that compounds of
this type will be enriched in the water and on subsequent
chlorination will lead to an increased formation of trihalo-
forms.

If methyl-substituted olefins are present in the humic
substances, it can be expected that they will be oxidized to

-------
                          - 328  -
Formation and  Degradation of
TRiHALOMETHANE  PRECURSORS
HA

f-^sfOH
IP

CH3

— *~ THMP — - — -


' krf? ' fast
+ OH* * ^^.--^r^i ^"^
*°3 » m *°3 s
3>C= o s'ow '•
i^H*"*~ +OH**
non THMP

Cr\
— U
1
r — n
XOH
,OH
-c=o
Fig. 1   Dependence of the product formation on the perfor-
         mance of the ozonization process  [2]
methyl ketones by direct ozonolysis  [1].  However, many of
the methyl keinnes are extremely stable to ozone  [3] .  They can
also be enriched in the water until they are oxidized further
by secondary OH* radicals.  This means that the formation
and degradation of methyl ketone-like haloform precursors
proceeds in accordance with laws- quite different from those
governing the formation of phenol- or resorcinol-like tri-
haloform precursors [2].           ...

Whether the ozonization-initiated oxidations proceed via
direct ozonolysis or via previously formed hydroxyl radicals
depends not only on the nature of the substrate molecules
but also on the nature of the ozonization process.  Fig. 2
shows that the time during which an ozone molecule is avail-
able for direct reaction is limited by the rate at which it
breaks down into radicals.  This rate is known to increase
with rising pH.  However, since the. decomposition of ozone
proceeds in addition by a chain reaction in which radicals

-------
                         - 329 -
                      Oo-degassing
                                 D-Reaction
**
k"
k
"\
L
/ T-OI TIVI
Jf
^
0
                                  R-Reaction
Fig. 2

         Dependence of the type of oxidation on the perfor
         mance of the ozonization process  [3]
         M: Oxidizable water constituents
         S: Scavengers
         R*: Free radicals
function as chain carriers-, many "water constituents are also
decisive for the decomposition" rate of the ozone [4].  Thus,
certain organic constituents (e.g. benzene in the 0.1 mg/1
range)  lead to an accelerated - ozone decomposition.   Other
constituents, such as bicarbonate ions or aliphatic alcohols,
inhibit the chain reaction by abstracting radicals from the
process [4] .   This gives rise to the situation that the ratio
of the direct ozonolysis to the radical OH" oxidation is
influenced by a wide range of constituents present in the
water.   On the basis of the chain reaction accelerating the
ozone decomposition, it can also be understood why the kinds
of reaction can depend on the istantaneous local ozone concen-
tration, i.e. also on the way in which the ozone is introduced,
If the results of ozonization processes are to be comparable,
an ozonization must be described very carefully.  It there-
fore seems to us to be very important that the greatest atten-

-------
                         - 33O
tion  be  focussed on a careful establishment of 'the process
arrangements and characterization of the water.   If this is
not done, the experience cannot be generalized.or extended to
other situations.
(1)  HOIGNE,  J. ,  BABER,  H.
    Ozonbedarf  und Oxidationskonkurrierwert verschieclener
    Wassertypen beziiglich der Oxidation von Spurenstoffen
    (this publication)

(2)  HOIGNE,  J.
    Discussion  of paper by M. Dore on "Influence of oxidizing
    treatment on the formation and the degradation of
    haloform reaction precursors"
    Prog. Wat.  Tech. 1_O (1978), discussion part, in press

(3)  HOIGWE,  J.,  BADER,  R.
    Kinetik  und Seiektivitat der Osonung organischer Stoffe
    in Trinkwasser
    Wasser Berlin '77,  Tagung der Fachgruppe Wasserchemie
    in der GDCh, Colloquium Verlag Berlin (1978), 261-276

(4)  HOIGNE,  J.,  BADER,  H.
    Beeinflus;sung der Oxidationswirkung von Ozon und
    OH-Radikalen durch Carbonat
    Vom Wasser  48 (1977),  283-304

-------
                          - 331  -
THE CONDITIONS OF OZONIZATION
J.P. Legeron
The number of studies and investigations on ozone and the
number of cases of the practical application of this gas
become greater every day.

However, the results obtained seem to be very variable,which
is perhaps in many cases due to omissions or errors, but
perhaps also to the absence of a common language.

In actual fact the aim of ozonization and the conditions of
the use of ozone could not be interlinked more closely. Ozone
is a chemical substance and as such reacts with other species
present in an aqueous medium: we can therefore speak of oxi-
dation kinetics and thus of conditions for maximum reaction
rates.

The temperature and sometimes also the pH of the water are
difficult to change, but the ozonization parameters are really
easily modified:

This is the situation, for example, with the ozone concen-
tration in the carrier gas, with the dose used for the treat-
ment', with the contact time, with the residual ozone concen-
tration in the gaseous and liquid phases, and with the life-
time of -this residual ozone in water.

The ozone can be added -in one portion or in several portions
and ;at one or more points in the treatment line.

The "contact can be intermittent or continuous.  Many studies,
for "example that by Monsieur Chedal at the Berliner Wasser-

-------
                            332  -
kongress in 1977» have indicated the' influence 'of these con-
ditions.

Let us first of all disregard the case of disinfection with
ozone, which is not-the object of this symposium.  Suffice
it to say that in this case the maintenance of a certain
quantity of dissolved residual ozone for some minutes makes
it possible to enhance the organoleptic properties of the
water, a fact that is attested by the readiness of ozone to
react with some substances still present in the medium.

While these reactions proceed relatively slowly, this is not
the case during the oxidation of very many organic and inor-
ganic compounds.  Depending on the type of the water, the
ozonization serves a different purpose at the start and some-
times in the middle of the treatment chain.

The case in question may be, for example, the oxidation of
iron and manganese, and here the contact  times are short and
the doses depend on pollution of the water.

In other cases we may -be concerned with an improvement of the
flocculation;  for this both the contact time and the dose must
normally be small.

Finally, we may be aiming at improving the biological degra-
dation, in this case the dose can be made higher, to introduce
oxygen into the water and to improve the bio-degradability of
the substances present.

Each case has its special features and for this reason rele-
vant trials must be performed either in the laboratory or,
better still,  in a pilot plant.

These trials must be as comprehensive as possible before any
qualitative or quantitative conclusions may be drawn.

-------
                          - 333 - -  •  •

Without going into the details of the process, which has
already been the subject of several contributions, I should
like to conclude with two specific examples.

     The first relates to the oxidation of manganese in a
     French river water;
     at constant contact time, quadruplication of the ozone
     concentration before it is added makes it possible to
     reduce the treatment dose and therefore the ozone
     production by almost 50%.

-    The second and last example relates to the oxidation of
     an iron/silica compound in a ground water of African
     origin:

     .splitting of this complex and the oxidation of the iron
     thus released cannot be achieved in a single ozonization
     step even with a high dose and a long contact time; the
     solution was found in several consecutive small additions
     of ozone alternating with aeration phases.

     This confirms that the results can be positive or
     negative, depending on the ozonization conditions.

-------
                          - 334 - • *;  •


DESCRIPTION OF•REACTIONS;BY GROUP•PARAMETERS
H. Lienhard and H. sontheimer
In this report I should like to discuss in greater detail a
problem that keeps recurring in the study of ozone processes,
namely how the changes caused by ozonization can be detected
and described.

The possibilities existing today for this purpose should be
discussed in the light of the experimental results obtained
in studies on the optimal ozone input, on which
Prof. Sontheimer spoke in his lecture on Monday.  Briefly,
such studies are aimed at establishing whether the ozone
should be added in stages, e.g. in a sufficiently large reaction
vessel by fine-bubble aeration, or whether it is more expedient,
as
possibly with a rotor, and then with an initially high concen-
tration to allow the reaction to proceed slowly.

To provide practical data from the results of these studies,
humic acids were used as model substances in the experiments.
This gave rise to the problem that the chemical structure of
humic acids is largely unknown and that during ozonization they
form products not directly accessible to analysis.  This means
that the reaction of the model humic acid with ozone cannot be
followed directly, so that the products formed must be charac-
terized   by measuring some accessible parameters.

To this end we used the 6 parameters shown in the left-hand
column of Table 1.

We shall now describe how conclusions can be drawn, from the
results obtained with these parameters, about the nature of
the reaction products and about their effect on the treatment.

-------
 TABLE 1   Description of reactions by group parameters
 Parameter
Measurable effect with
stepwise addition of
ozone as opposed to a
single addition
Interpretation of the
effect
 Spectral absorption co-
 efficient at 254 nm
Decrease
Degradation of the
double bond system
 DOC
No difference
No increased CO,
formation
i Flocculation effectiveness  Improved relative
:                             flocculation activity
                         More polar molecules
                            w
                            w
 Molecular weight distri-
 bution
Displacement of the
mean molecular weight
towards smaller values
Smaller molecules
 Adsorption on
Displacement of the
isotherms to the-left
More polar groups on
the molecule >
 Haloform formation
 potential
Less trihalomethanes
Degradation of the
electrophilic centres,
fewer double bonds

-------
                          - 336 -




The middle  column shows the effect measured  for  the  parameter

on the  left,  obtained with fractional addition of  the ozone.

The right-hand column lists the conclusions  that can be  drawn

from  this about the change in the organic  substances.



From  the observed decrease in the spectral absorption co-

efficients  at 254 nm we concluded an intensified degradation

of the  double bond system with intermittent  ozone  input.  The

fact  that the DOC is the same for both modes  of  ozone input

means that  only a different change in the  humic  substances

occurs, which also happens in practice, since the  effective-

ness  of flocculation is enhanced by stepwise  ozone addition,

as shown in Fig.  1.
    9r
 O OP
 QJ
 D-7
 U) 4J '
   c
 QJ Ol
 si -H 6
 JJ U
   •H
 c «w ,.
 ••H »M 5
   at
 at o
 in u ,
 m   4
 
 T3 D
   M
  W
  G
o Ruhr humic acid

a Lake Constance
  humic acid
            0.4  0.6   0.8  1.0
1.4   1.6  1.8  2.0
    V  2.4   2.6  2.8
    mg O^/mg acid
Fig. 1   Relative  improvement of flocculation effectiveness

         by stepwise  ozone  addition for two humic acids

-------
                          - 337,-
This figure illustrates, the  relative improvement of the flocc-
ulation effectiveness due  to the intermittent ozone input. It,
can be seen that e.g. for  the Ruhr humic acids after stepwise
ozonization by flocculation  alone the spectral absorption co-
efficient is reduced by up ,to 7.5% more than in the case of
a single addition.  The same applies to the humic acid from
Lake Constance.

To understand this  effect  the molecular weight distribution
must be measured, as can be  seen in Table "I -  The distribution
is displaced towards smaller molecular weights.  The absorp-
tion experiments additionally performed on CaCO^ confirm an
increase in polar groups compared to the case of the single
addition of ozone.  The haloform formation potential is also
reduced  (Fig. 2).
                ° Single ozone addition
                f; Stepwise ozone addition
                  Ruhr humic acid
     0  0,2 0,4 0,6  up Ip 1,1  V1  *£ V» V V
                          .rag O,/mg acid
Pig.  2    Dependence of .the haloform formation potential on
          the  amounts of ozone for-—two different kinds of
          ozone addition

-------
                          - 338 -
The figure shows the amounts of trihalomethanes formed, during
the chlorination as a function of the ozone input.  Prom
the course of the curves it can be seen that stepwise ozone
addition reduces the haloforni formation potential more
strongly, and this indicates that fewer electrophilic centres
and so fewer double bonds are available to the electrophilic
attack of the chlorine when this type of ozonization is used.

If the information yielded by each individual parameter
listed in the right-hand column of Table 1 is compared, it can
be seen that the data provided by each parameter are supported
by the other results.

For example, the results of the flocculation experiments can
be explained both by the change in the molecular weight dis-
tribution and by the increase in the concentration of polar
groups.  The two effects run in opposite directions and lead
to an explanation of the different behaviour in the two modes
of ozone addition.

Without going into further detail of all the interesting
relationships, from the results presented it can be concluded
that a combination of a number of experimental parameters can
lead to a better appreciation even of small effects and to a
deeper understanding of the processes taking place during
ozonization.

-------
                         -  339  -


PRODUCTION OF OZONE FROM OXYGEN
G. Uhlig
The problem appears trivial: a molecule consisting only of
oxygen atoms can likewise only be produced from oxygen.
The production of ozone from air succeeds only because, in
spite of environmental loading, even in regions of accumul-
ation, air always contains 21 vol-% of oxygen.

However, it is also this oxygen concentration that finally
limits the yield and the efficiency of even the best ozone
generators.  This applies above all when not only the amount
of ozone produced but also the ozone concentration achieved
is the main criterion, because the dissolution of ozone in
water is subject to the Henry-Dalton law, according to
which the solubility of ozone is proportional to its par-
tial pressure, and thus to its concentration, in the gas
phase.

According to the laws of reactions kinetics, there is no
doubt that the yield and efficiency of-the production of
any material increase with the increasing concentration of
the starting components.  Some measurements, here cited only
as examples, for series-connected ozone generators, dimen-
sioned for the production, of; -ozone;.- from air illustrate this
point better than the corresponding reactions and equations:
                      '  .•*•„'•**      ^
Fig. 1 shows, on the basis of Masschelein's measurements
(1), the increase in the hourly 'production of ozone
with   increasing ' oxygen •"•• concentration   at   a   constant
gas throughput for various' power levels of an ozone gener-
ator. At the same time, as can be seen in Fig. 2, the
energy expenditure on the production of 1 g of ozone is
greatly reduced.

-------
  kg Oi/h
          20
                       Ozonour 2
                               Vol %02
                 40
60
                               80
Fig.1  03 production/h in dependence on
        the  C>2  concentration.
        (after W.  Masschelein, T.S.M.
        L'EAO 71  (1976) 385)
       Ozoneur = ozone generator
                                 Wh/g03
                                                         15
                                                         10
                                                            70kWh
                                                            50 kWh
                                                                Ozoneur 2
                                          20
                                                                Vol % 02
60
80
                                Fig.2  Specific energy requirement
                                        of 03  production in dependence
                                        on the 62 concentration.
                                        (after  W. Masschelein, T.S.M.
                                        L'EAU  71 (1976) 385)
                                                                                                    to
                                                                                                    *»
                                                                                                    O

-------
                       - 341 .- .;•
  52-
  50-
  48-
  46 -
  44 •
  42 •
  40 •
  38
  36
  34
  32 -
  30-
  28-
  26-
  24-
  22-4
  20-
  18-
  16-
  U-
  12 -
100 Vol. V.02

80 VcI.*A02

50 Vol.7.C
 Fig.  3
 03  concentration produced  in
 dependence on the gas flow at
 various Oz concentrations,
(after M. Bredtmann, Wasser/
 Luft  und Betrieb 11(1974}  605)
         0,2   0,3    0,4
Higher concentrations  of ozone are generally achieved  by
reducing the gas  throughput of the ozone generator. Fig.  3
shows a diagram taken  from Bredtmann  (2), in which
the concentrations  of  ozone obtained with a series-
connected tubular ozone generator during the change-over
from air to pure  oxygen is demonstrated.
Here too, as  can  be seen from Fig. 4, the specific  energy
expenditure for the production of increasing ozone  concen-
trations on going over from air to pure oxygen  is essen-
tially reduced.

-------
                           342 -
     V.'h/gOj
     20
     16-

     U-

     12-

     10-
     9-
Fig. 4
                        50 Vol. %02
                                 BO Vol.%02
                                       100 Vol. % 02
                                                   g Ch/m3
        15
     20
                   25
                30
35
                                             50
                                          55
Specific energy requirement in dependence  on  the  €3
concentration produced at various O2 concentrations.
(after M. Bredtmann, Wasser, Luft und Betrieb 11(1974)
605}
Even when  the starting gas contains only 5O to  9O vol-%
of oxygen  it is an additional advantage for the dissolution
of ozone in  water that the correspondingly smaller propor-
tion of nitrogen still permits a gasification of the water
under  pressure, which in the case of ozone production from
air would  lead to supersaturation of the water with nitrogen,
However,  all  these advantages do not cover the costs of the
oxygen  consumed if this is not returned to the production
after the ozone has been washed out.  Therefore, if the
process is to be economically viable, gas circulation must
be  installed.  On account of the improved ozone dissolution,
however,  only a partial water stream needs to be gasified
with ozone for this purpose,  and expensive structures and
installations for the gasification of the whole amount of
raw water become unnecessary.

-------
                         - 343 -
By means  of  the  production  of high ozone  concentrations in
the  gas phase  (5O-9O  ozone/m  is  nowadays a realistic figure)
and  by the use of  pressure  in the gasification of the water
in space—saving  scrubbers or columns,  ozone concentrations
of 2O-80  g/m of water  can  be reached.  Accordingly,  the
dissolution  of 2-3 g  of ozone per m  of raw water.still
requires  only the  gasification of a partial water stream
amounting to 3-lO% of the total water.  The highly concen-
trated partial stream is then mixed with  the main stream in
such a way as to ensure a good distribution and a sufficient
reaction  time.
The apparatus and the'physico-chemical principles of this
process have already teen given in detail by Axt  (3) 1958
in his dissertation "An indirect process for the  ozona-
tion of water".  This already included calculations for
the design of ozone-introduction columns, calculations of
the energy yield during the production of ozone from oxygen,
and a description of a specialized ozone generator to be
operated at 6-7 bar that provides for gas circulation at
a constant excess pressure.  Since such ozone generators
are not produced commercially, the gas circulation must in
practice be operated with two pressure levels: an optimal
pressure for the dissolution of ozone and an optimal pressure
for the production of ozone, which in the majority of cases
is restricted to 1.6 bar with today's ozone generators.

A large-scale realization of the process took place 8,
years later for the first time at the Duisburg AG municipal
works with an ozone plant designed for a throughput of 250O .m
of raw water per hour.  This plant was commissioned in 1966
in the Wittlaer waterworks for the treatment of Rhine water
filtrate.  Simon and Scheidtmann (4)  1968 have reported in
detail on the extensive preliminary trials on adaptation of
the process and on the technical data of the main plant.

-------
                          - 344  -* '
 The process  scheme of this plant  is  given in Fig. 5: fresh
 oxygen is  led out of a 5OOO m   liquid oxygen tank (bottom
 left in the  figure)  via an evaporator warmed by air and
 directed to  the gas circulation before the production  of
 ozone.  The  ozone production at 1.O5 bar in 4 tubular  ozone
 generators (only one unit is'shown'in each case) supplies
 an ozone concentration of 4O-6O g/irt3.  The pressure is then
 raised to  1.8 bar and the gas-mixture' 2 is conveyed to the
 ozone scrubbers, made in the form'of'packed columns,  in
 Which the  partial stream of: water -  "about 1O%  of the  whole
 water treated -  trickling in  countercurrent through  Raschig
 rings undergoes gasification.
 The unconverted mixture leaving the scrubbers at the top
 is directed  to a drying plant  with a cooled condensate
 separator  and returned for use in the production of ozone.
                   Ozone scrubber-
                              Mixing and reaction vessel
                                                 Filter plant
        Dryxng plant
                                                   	 Multilayer
              Liauio~Qxyqtn tank
Sa fety chlor™ De-acidt fl-
 irtation      cation
Fig, 5  Diagram of the ozone plant  at Wittlaer Waterworks  III

-------
                        - 345 -
The partial stream of water, containing 1O-25 g of ozone/m
and described in the diagram, as "ozone-rich water" is
                                              >
added to the raw water in,two reaction tanks after the
pressure has been raised to-8 bar.  The ozonized raw water
coming out at the bottom through a concentric drainage
funnel passes 2O two-stage filters with a total filter area
        2
of 2O6 m  per stage, consist-ing of a multilayer filtration
for the removal of the flocculated.ozone-oxidized products
and an active carbon stage, connecting safety-chlorination
with chlorine dioxide and deacidification with a solution of
caustic soda.              •  •

The external appearance of the Duisburg ozone plant is
illustrated in

Fig. 6, which shows the two scrubber columns for the disso-
lution of ozone and the pumps for increasirg the pressure,

Fig. 7, which shows the control panel, the ozone generators
(right), and the reaction tanks (left) in the background,
and

Fig. 8, which shows an external view of the whole plant.

-------
-•: Vff :«wti=^-r -gff?-'.--'-'-• i^** = m¥T'- --1K ' *i«ri!"!!BT.-pJf«?^-* •"" • ,"

•;|
       •«,• sHfi^at^'             ~ • *
= , 1 ^ 5 ^^Ugi 'i^'r^-tTffil • Sitfis^S^'^r-«?':lr:LJ"-.'«^ ^. .' .;" ; \ •'
 6   Ozone  scrubber  column  and pressure

     pumps  of  the  ozone  plant
                                                                                              I


                                                                                             U)

-------
                 . ;i: v
                  .-f      >c
Fj-g> 7  Control panel,  ozone generators   »**-*
        and reaction  tanks  of ozone olant"" *> *

-------
RjV-' ;-*:, i<-yHi*:-  '*••'• 0.'*i-.--:,'f If-',/ i"-..  •:i'Vr'  •-.- *   ift..^ ,U

^SMn^
 •;>-^ T^s', T-Tfc.^ »£..;• ;-••"•• t '
-" • •-•* rK-^t '.?;•• ;."> ., .?:. .P. .:
•fe^iSiill-*1
-., ^•riS-^'^r'-"---^.'^
                                                                                                                                         I
                                                                                                                                        w
                                                                                                                                        00
                                                                                                                                         i
                          Fig.  8   External  view  of  the ozone  plant

-------
                        - 349 -


Control of the ozone production only by the tension in
the ozone generators at constant gas throughput is not
optimal, since the ozone concentration is then diluted
again.

However, the control also  of the amount of gas in circul-
ation, with the ozone concentration regulated to a constant
value, already envisaged in this plant, has not been  ap-
proved in the proposed form and is the object of further
improvements, as is the design of the columns.

It should be emphasized that in this process the raw water
from the feed pumps of the wells onwards undergoes the
whole treatment already under distribution pressure, and
the pressure need not be reduced for the ozone treatment.

In the selection of the pressure for operating the columns
it must be remembered that the Henry-Dalton law applies not
only to dissolution of the gases  —  this concerns above all
oxygen in addition to ozone  -  but also to the release of
gases dissolved in the water.  The main component dissolved
is still nitrogen, corresponding to its partial pressure in
the air.  However,, in a pure oxygen/ozone mixture the par-
tial pressure of nitrogen is zero, and thus does not corres-
pond to the distribution equilibrium.Consequently, a partial
pressure of nitrogen corresponding to the nitrogen content
of the water is rapidly set up in the gas circulation by
outgassing.  The concentration corresponding to this par-
tial pressure depends on the partial pressure of the oxygen
and therefore on the selected total column pressure, which
in turn determines the dissolution of oxygen in the partial
stream of water.

These relationships are presented in Fig. 9 for nitrogen-
saturated water in an easily followed diagram due to
Albrecht (5): when the pressure is raised to 8 bar the

-------
                          - 350.r
                      f • % * 5  . i s  t *- s   f. -  < L ,, -  v * •,   : , «    ... . - - ~
 nitrogen content in  the gas circulation can be reduced to
 about 1O%.   As shown by measurements carried out by Rosen
 (6),  and also by  Cromwell and Manley  (7) ,  a smaller con-
 tent  of nitrogen scarcely  improves the  ozone production
 efficiency, and furthermore, increasing the pressure also
 reduces the column layout  and dimensions.   According to
 Greiner and Grxinbein (8,9), at 5-7 bar  8-10 theoretical
 plates are necessary for almost quantitative washing out of
 the ozone.
           Oj   Nj
          mg/l Vol.%
          1000
          900
          000 -
          700-
          500
          400-
          300-
          200-
           100-
100 •

90-

80-

70-

CO-

50-

40-

30-

20-

10-
                                For ",-saturat-ed water
                               at 10° C"
                     j" enrichment
                                            bar
                   1 2  3  4  5  6 7 8 9 10  It  12 13 «
Fig, 9  Distribution equilibrium of nitrogen  (vol-%)  in
        oxygen and of oxygen  (mg/l)  in water
        (after E.  Albrecht, Dechema - Monogr.  75  (1974)  343)

-------
                         - 351"-

The pressure elevation is,however restricted by the in-
creasing oxygen dissolution, the limit being determined
by the oxygen content and the ozone consumption of the
raw water and also by the oxygen demand of the after-
connected treatment plant.  To avoid this situation by
a subsequent aeration of the water to drive out the excess
oxygen necessitates considerable additional expenditure
and is not practicable for a water treatment under distri-
bution pressure in a closed system.

For open plants, on the other hand, even a slight enrich-
ment of the aerial oxygen, without using gas circulation
but with the pressure produced by the greatest possible
depth of immersion of the gasification, can have certain
advantages, as reported by Masschelein (1) and by Rosen
(6) .

For oxygen enrichment of the air Rosen (6) recommends a
"pressure swing oxygen enrichment" with the use of a
molecular sieve in 2 towers which are run alternately
under different pressures.

Summary
1)  With the same energy consumption of the ozone
    generators, at least twice the amount of ozone per
    hour can be obtained, in at least twice as high
    concentration, when the ozone is produced from
    oxygen and not from air.

2)  Corresponding to this increase in concentration,
    the ozone dissolution also increases for all types
    of gasification of the water, and on an additional
    application of pressure in suitable columns the
    dissolution is higher by a- factor of 10-30 than in
    the case of open gasification of the water with
    ozone produced -from air.

-------
                        — 352 -
3)   It is thereby possible to limit the gasification
    with ozone to a partial stream of water amounting
    to 3-lO% of the raw water to be treated.

4)   Since the oxygen is circulated none of the remain-
    ing ozone need be wasted. The ozone is almost
    completely dissolved or goes to the return gas.

5)   At most 1/1O of the amount of gas that must be
    transported and dried in the case of ozone produc-
    tion from air is conveyed to the gas circulation in
    the case of production from oxygen. This reduces the
    costs of gas drying and transportation, together
    with the energy balance for ozone production and
    costs of cooling the ozone generators.

6)   In the Duisburg process of ozonization of the drink-
    ing water, which is based in its concept on Axt's
    fundamental work (3), these advantages were already
    realized 12 years ago corresponding to the state of
    technology at that time. So far it has been the only
    conceivable process for ozone treatment of the water
    under distribution pressure in a closed system, and
    work on its improvement is continuing.

-------
                       -. 353> -
(1)   MASSCHELEIH,  W. .
     Perspectives  de 1'ozonation de l'e-au au depart d'air
     enrichi en oxygene
     T.S.M.L'EAU 21 (1976), 385-399

(2)   BREDTMANN, M.
     Bildung von Ozon und Konseguenzen fur die Konstruktion
     von Ozonerzeugern        •
     Wasser, Luft  und Betrieb _1_8 (1974) , 6O5-609

(3)   AXT, G,
     Uber ein indirektes Verfahren"zur Wasserozonisierung
     ur.d dessen apparative und physikalisch-chemische Grund-
     lagen
     Dissertation  Universitat. Karlsruhe (1958)

(4)   SIMON,  M., SCHEIDTMANN, H.
     Die neue Ozonanlage der Stadtwerke Duisburg.
     gwf-Wasser/Abwasser 109 (1968), 877-882

(5)   ALBRECHT,  E.
     Die Anwendung von  reinem Sauerstoff fur die Wasser-
     behandlung
     Dechema-Monogr. 75 (1974), 343-356 .

(6)   ROSEN,  H.M,
     Ozone Generation and its Economical Application in
     Wastewater Treatment
     Water and Sewage Works 119 (1972), 114-12O

(7)   CROMWELL,  W.E., MANLEY, T.C.
     Effect  of Gaseous  Diluents on Energy Yield of Ozone
     Generation from Oxygene                    •  .
     Ozone Chemistry and Technology, Washington (1972),
     304-312

(8)   GREINER, G.,  GRtlNBEIN, W.
     Gewinnung von ProzeBwasser aus Abwasser
     Dechema-Monogr.TJS  (1974),  399-406

(9)   GRUNBEIN,  W,
     Ozonisierung  von Abwasser
     Chemie-Ingenieur-Technik 46 (1974), 339

-------
                       - -354 - -
MEASUREMENT OF THE OZONE-DEMAND
C. Gomella
A - INTRODUCTION

The doses of ozone applied to pre—treated water, i.e.
introduced into the ozonization reactor, which appear
in the bibliography are difficult  to compare with one
another, and thoseused in laboratory trials cannot as
a rule be extended to industrial practice.

The doses of ozone giving the same end result can vary
considerably as a function of the  experimental conditions
and the experimental or industrial apparatus used. This
is due to the fact that the ozone  can be broken down as
follows ;•->••

      T -= D + r + A + p
where T is the dose of ozone introduced into the reactor,
      D is the amount of ozone actually used up in the
     ;  ; • • •• •                               '-
        various reactions (the ozone-demand),
      r is the residual free ozone maintained in the
        water within the reactor (entrained by the
        water issuing from the reactor),
      p is the ozone lost by entrainment with the exhausted
        ozonized air leaving the reactor,
and   A is the ozone consumed through auto-decomposition.

 A technical and economical analysis of an ozonization
 process, such as the application of a laboratory or
 pilot result, cannot be undertaken without a reasonably
 precise knowledge of the elements making up the treat-
 ment dose T.  The problem is a complicated one, for

-------
                      • ' -  355  -


these elements are not  all independent of one  another;
in particular:            „  .  •

D is a function of the '-quality.,of the water, the  time of
the treatment, the value of the O^ residue, and of  the,
concentration of the ozonized -air.

r is imposed by the investigator, as a function of the
desired result and also as, a function of its effect on
D and A.                 ' . '•- . ,

A is dependent on .quality of the water, the duration of
treatment, r, and the concentration of the ozonized air.

p is dependent on the internal-structure of the reactor
and on the concentration of the ozonized air.

The present communication outlines a method used  in Fran6e
for fifteen years by the author and his associates,
developed specially to  surmount problems of interpretation,
From a pragmatic point  of view, this method has proved
very effective and has  enabled experimental results  to
be transposed to industrial conditions with a  good  degree
of precision.               .  „

B.    PRINCIPLE                 '
When the amount of ozone actually introduced into the
water has been determined, D and A   are calculated by
measuring r  ...  at different-times.

Before the calculation can be made,  a hypothesis about
the form of the auto-decomposition law must be formu-
lated.  The many studies mentioned in the bibliography
give various formulations, notably 2nd or 3/2-power

-------
                       - 356
laws.  In practice, it has been shown that a law of the
form:
                  In ~  = a(t - tQ)     a < 0

where  In is the natural logarithm,
       r  is the residue at time t ,,
       r is the residue at time t,
and    a is the auto-decomposition coefficient, is quite
sufficient in current practice in the case of slightly
polluted water with the usual pH  (6 to 8) . Without wish-
ing to give a rigorous scientific meaning to this law, it
appears that its application in the given case enables
one to arrive at predictions and calculations of industrial
installations .

It should also be noted that the method is not specifi-
cally connected with this, form of the law, and that it
is sufficient to replace the chosen law by one determined
experimentally and better adapted to the case in question:
r = f (a,t), to be able to apply the method in its prin-
ciple by simply modifying the mode of the calculation.

C.    THE DIFFERENT STAGES OF, THE METHOD
A quantity of ozonized air of a known concentration and
a quantity of water are introduced into the same flask,
after which they are stirred vigorously and the residual
ozone is measured at successive moments in time.

Two measurements are sufficient to determine the auto-
decomposition coefficient, that is, r, at time t, and r?
at time t0 :
         2          r
                 in     = at
 {t  is taken to be the time or\- :n)
  o

-------
                        - 357 -'
                      .r
                   In —   = at,,
                      r2
whence:                   ,r~
                       In ^±
                   a =    r
and                r  = r, e   1  • •
                    o    "1	

The ozone demand D can then be determined,  provided 'that
the initial concentration of the ozone  introduced  into
the water  (C ) is known:  ,
            o
                   D = Co ->ro-   '              '

C  is easily calculated from the respective volumes v and
 O                          ,                           ;
V of the ozonized air and the  treated water,  from  the
'ozonized air concentration, and" from the  distribution co-
efficient S  (Henry's law) of the ozone  between  the air"
and the water at the temperature of the experiment, which
is conducted under atmospheric pressure:
                    C  =  c
                     o
                         i + v  • •

If  t  is the time of the ozone "treatment  in  the  reactor, '
and  r  is the residue determined by  the  investigator,  then
the quantity of ozone to be introduced  into the water will
be  (in the case where the amount of residue is kept con-
stant in the industrial reactor):

                   D+r+rat=D+r (1+  at)

The treatment dose will depend  on the loss of ozone en-
trained by the ozonized air leaving the reactor.  It de-
pends essentially on the form of the  reactor, the time
of contact of the ozonized air  and the  water, and the

-------
                        - 358 -C~-  ••


ozone residue. This is why i-t is highly desirable to
conduct the laboratory experiments with a residue similar
to that applied in practice. The loss  p  can be esti-
mated, to be in the region of lo to 3O%, and under
these conditions:

         D * r (1 + at)   4 min.

-------
                       - 359
                        •APPENDIX

      DETERMINATION OP THE CHARACTERISTICS OP WATER
      IN RELATION TO OZONE' AND OF THE INDUSTRIAL  ".
      OZONIZATION DOSE                      • •   ' :  .
1.    PRINCIPLE
The aim of the operation is to determine:
-     the instantaneous chemical ozone demand;
-     the specific auto-decomposition coefficient; '.•
-     the industrial ozonization dose.

To this end a known quantity of ozonized air is brought
into intimate contact with a fixed volume of the water
under examination, and the residual ozone dissolved in
this water after contact times of 1 .min and 4 min. is
measured.

2.    DESCRIPTION OF THE EQUIPMENT USED           '
      2.1. TRAILIGAZ LABO 66 laboratory ozonization unit
      2.2. Volumetric flask                    •  ••'•  •
The ozonization reaction takes place in .a 6OO ml volumetric
flask with a graduation at 50O ml.

A ground-glass stopper fitted'with a tap enables the flask
to be completely filled with the water being examined.

A side tap, placed on the neck of the flask below the
5OO ml mark, enables the contents of the flask to be
drained off down to 50O ml.

-------
                         -  360  -?
Fig. 1  Ozonization unit -   -. .   ,

        1 = wattmeter;  2 = flow-meter;   3  =  manometer;
        4 = dry air inlet;  5 =  sampling  valve;
        6 = compressor;  7 = Mohr  clip; 8 = free  air
   500ml -
                 41
                 31
                 21
                 11
Fig. 2
Measurement of the
ozone demand;
special flasks

-------
                       - 36V'-  -


3.     PROCEDURE

      3.1.   Method of obtaining the ozonized air


      Determine flow rate and the power consumption*.

      Start the cooling water circulation of the ozoni-

      zation unit.

      Start the air compressor.   •

      Regulate the air' flow rate by means of the sampling

      valve (the flow rate valve being closed).  The

      delivery-rate is read off a flow-meter (reading at

      the top of the ball).

      Regulate the air pressure by means of the pressure
                                                    2
      valve; the pressure should be set at O.5 kg/cm  (the

      calibration curves of the ozonization unit were

      plotted for this pressure).

      Apply power to the ozonization unit.,
   The concentration of ozone in the ozonized air produced
   depends on two parameters: the air flow rate and the
   power consumption. The calibration curves of the ozon-
   ization unit enable a flow rate and a power consumption
   corresponding to the desired concentration of ozone
   to .be determined. The choice of the latter depends
   on the nature of the water being studied, the aim being
   to obtain a residue of O.4 mg of ozone per litre of
   water after a water-ozone contact time of 4 min (see
   Section 3.2). In cases where this residue is below a
   value of O.3, or above a; value of O.6, it is necessary
   to repeat the experiment with a-higher or lower ozone
   concentration of the air. In the case where the highest
   possible concentration of the ozonized air does not
   give a residue of O.3, a special 6 litre flask must
   be used, which enables the air/water volume ratio to
   be varied from 1/5 to 5/1, this ratio being first made
   1/2 and then higher if necessary.  By way of an indi-
   cation, a content of 10 .mg of • ozone per litre of air
   is suitable for only slightly polluted water (such as
   well water).

-------
                          -  362 '-  '

    Turn the  power "hanVMheelclo'ckwise ' to obtain the •'>'*
    desired power consumption; this  is  read off the
    wattmeter.                  ;   ,.  •
    Wait for  five minutes to. achieve stable flow rate
    and power consumption.
          400
          3OO
          2OO
          1OO
                20W 30W   SOW 60WTOW    10QW
                                        power absorbed in
             fl
             air: dew point
                 - 50°C
             pressure: O
                     kg/can'
C3onc.in mg O-,
air
                             10
                                     15
                                             20
                                                      25
Fig. 3  Mean  air production curves  for the LABO 66 unit
    3.2.  Performance of,the ozonization

    Pill the  flask described in Section 1.2 With the water
    to be ozonized.
    Connect the  ozonized air pipe branched to the sampling
    valve to  the upper arm of, the ground-glass stopper?
    the end can  be partially closed by, means of a Mohr
    clip opening to the atmosphere..

-------
                     - 363 -


When the Mohr clip has been adjusted so as to produce
a steady flow of ozonized air to the atmosphere
during the following operation, open the side tap of
the flask so that the water level descends to the 5OO
ml mark. The gas volume on top is now occupied by
ozonized air at a concentration corresponding to the
ozonizer setting.

Close the tap on the ground-glass stopper and dis-
connect the ozonized air inflow.

Shake the flask vigorously for 2O sec, timing this with
a stopwatch. (The ozonization reaction and the estab-
lishment of an ozone equilibrium between the air and
the water take place during these 2O sec).

Let the flask stand for 2 min, then tilt it so that the
water clears the side outlet-; the remaining ozonized
air is expelled by blowing air into the end of this
outlet. Determine the residual ozone in the water as
described below.

Repeat  the experiment, this time letting the flask
stand for 4 min instead of 2  min.

Repeat  as above, this time letting the flask stand
for  8 min.

3.3.  Determination  of the residual ozone
Immediately after this time  (2, 4, or 8 min), place
several crystals of potassium iodide  in  the  flask. The
residual ozone  oxidizes- the iodide to iodine. Pour the
5OO  ml  of the  iodine solution into a  conical flask,
and  2 ml of 1:2 E^S.O., and titrate with  sodium  thio-
sulphate.,

-------
                        -  364  -    -    ,


Refer to the method of  determination  of residual ozone
in the water.

4.  CALCULATION
    4.1.  Determination of the auto-decomposition
          coefficient  (-a)
Find the mean of the three values obtained with the aid
of the equations below* :
               ,  . -1,   1 ,   r4    1   .  r8   1 ,  r8
           -a  (mm  5 = ~- In  —  = T   In — = T In —
                        z     r,j    4     r«   o    r»j

where r,,, r., and r,, are the  residual  amounts of ozone
after contact times of  2, 4,  and  8 min respectively.

   4.2.  Determination  of the immediate ozone demand (D)
By definition, the immediate  ozone demand is:
                  D = C - r
                        o    o

where C  is the initial ozone concentration in water,
      introduced by means of  the air-water contact; it
      is calculated as  in Section 4.21,
and r  is the residual  concentration  of ozone in the water
     o
      at time 0, calculated as in Section 4.22.

   4.21.  Calculation of CQ

                 C  = cv
                  o
where v is the volume of ozonized air in contact with the
        water
      V is the volume of water in contact with V-, ,

 *  If  rg  is  less  than O.2,  the following value will  be  retained;
         a =  *   In  r4
              Z       r2

-------
                         -  365  -
30 -
20 -
10-
       c is the con'centration of ozone in the ozonized air,
       S is the distribution coefficient of the ozone between
         the water and the air? the value of this coefficient,
         which is a function of the temperature, is deter-
         mined with the aid of Fig. 4 in the Appendix.
                      Fig.  4
                      Henry's coefficient (S)
                      as a  function of
                      temperature 0
                      S = f(0)
  0   0.1   0.2  0.3  0.4   0.5  OB   0.7   s
    4,22.  Calculation of r
                           o
 r  is found from the equations below  (take  the mean  of
 the 3 values otbained, or of the first two  if r0  is  less
                                                o
 than O.2);
          In
= 2a
             _2.  = 43
          in
              8
= 8a
 where  a  is the auto-decomposition coefficient calculated
 in Section 4.1 and r«, r., and r0 are the residues of
                     ^4       O
 ozone after respective ozone-water contact times of  2,  4,
 and 8 min.

-------
                           366 -
OZONE INPUT
W.J. Masschelein
As far as the mode of action and the passage of ozone into
water are concerned, at least two essentially different
mechanisms must be taken into consideration:

1)   contact with bubbles of strongly ozonized air;  we were
     able to show (cf. our report to the Berliner Wasser-
     congress in 1976) that as regards the bactericidal
     action of ozone this mechanism plays a very important
     if not the main part.  Similarly, the literature data
     on the use of ozone in the preliminary treatment of
     water can be interpreted as favouring micelle formation.
     This mode of action is illustrated by the rapid bacteri-
     cidal action obtained during the passage of the ozone
     from the gaseous into the dissolved phase.

2)   continuous, stepwise, and slow action of the residual
     dissolved ozone; this mechanism is particularly suitable
     for combatting impurities more resistant to oxidation,
     for example detergents.  This mode of action corresponds
     to first-order kinetics with respect to the concentration
     of the dissolved ozone and also seems to be of decisive
     importance for the viricidal action.

Therefore,during the planning and layout of an ozonization
plant these two basically different action mechanisms must be
borne in mind: passage of the ozone must be ensured, e.g. with
the aid of, turbines, and a relatively short contact time  (less
than 1 min) is necessary; the effect is thus achieved by the
contact with bubbles of strongly ozonized air.  On the other
hand, the slow mode of action of the residual ozone in the
after-treatnient requires a time of at least 6 min.  During
this period a concentration of 0.2 to 0.4 g O^/m  must be
maintained.

-------
                        - 367 -,  • .   .


OZONIZATI.ON BY-PRODUCTS AND THEIR REMOVAL BY COAGULATION

J.C. Kruithof


1.  Introduction

In surface water, taking the river Rhine as an example,
many organic micro-pollutants may be found. One of the
most important groups of pollutants is the fraction of
the aromatics  (1-3). In this fraction many alkylated,
hydroxylated, chlorinated and nitrated mono-aromatics
and poly-aromatics can be present. These impurities must
be removed in the process of drinking water treatment.
One of the processes to remove these compounds is ozo-
nization. In this paper the ozonization of phenol,
naphthalene and phenanthrene will be discussed. In
particular, the production and stability of organic
peroxides will be mentioned. Furthermore, the formation
of dialdehydes and their conversion to carboxylic acids
will be discussed. The ozonizations have been carried out
at a pH of about 7, so the initial reaction of ozone will
be an electrophilic substitution and not a reaction of ozone
with hydroxylic ions.

In the second part of the paper some examples are given
for the removal of carboxylic acids produced during ozo-
nization by coagulation with aluminium salts. The removal
of oxalic acid, o-phthalic acid and 1,3,5-benzene tricarbo-
xylic acid will be reported. The models for removal are
based on the hydrolysis of the metal salt used, the disso-
ciation of the carboxylic acid, the complexing of the
metal ion with the carboxylate ion and the solubility of
the metal hydroxide used.

-------
                        -  368  -


2.  Ozonization of some aromatic compounds

2.1  Ozonization of phenol
r
Many authors describe the ozonization of phenol in water.
Bauch' et al. (4) assume the formation of an ozonide which
is converted to smaller molecules, and a direct oxidation
by which the benzene ring is ruptured. Eisenhauer (5-7)
finds catechol and o-benzo quinone as the first reaction
products. Gould  (8, 9) states three mechanisms: hydroxyl-
ation, cleavage of the benzene ring and the production of
polymeric compounds. According to our own investigations,
two major reaction paths occur, the first of which, taking
place for about 30 %, is hydroxylation producing'catechol
and hydroquinone as reaction products. The concentration
of these products is given in Figure  1. Both concentrations
reach a maximum after an ozone consumption of 3.6 • 1O
moles (0.4 moles of ozone/mole of phenol originally present).

In addition, there is a rupture of the benzene ring for 70 %,
producing an aldehydic and an acid hydroperoxide. Both per-
oxides prove to be unstable and will either cause an elimi-
nation of H_02 or rearrangement. An elimination of H O  will
convert the aldehydic peroxide to glyoxal. When a rearrange-
ment takes place the aldehydic peroxide is converted into
two moles of formic acid, the acid peroxide rearranges to
one mole of formic acid and one mole of CO . The concen-
tration of glyoxal and H_O2 is given in Fig. 2, and that of
formic acid and CO  in Fig. 3. The concentrations of glyoxal,
                  £
H0O0 and formic acid reach a maximum after an ozone consumption
                -3
of about 26 • 10   moles  (2.9 moles of ozone/mole of phenol).

-------
                          - 369 -
1O

A
                           20

                       ,IO"*(")ole$)
Fig. 1  The  concentration of catechol  and hydroquinone
        as a function of the ozone-uptake
        (x = catechol;   o = hydroquinone)
   10.0



    8,0
 u  6,0
 c
 O
 u
    4,0
    2.0


      O
       O   10   20  30  40   50  60  70

       	fc— A  03" JO'Violes)


Fig.  2   The concentration  of glyocal and H202 as a  function
         of the ozone-uptake
         (x = glyoxal?  o = H2O2)

-------
                         -  370 -
c
o
u
50,0

40,0

30,0

20,0

10,0
   0
                                         5O.O
                                              o
                                         40,0 £
                                  30,0

                                  20,0

                                  10,0

                                     O
        0  10   20   30  40  50  60  70  80
        •	•	   A  03 Jo'^rnoles)
                                              O
                                              U
Fig. 3  The concentration of formic acid and the C02-
        production as a function of the ozone-uptake
        (x = formic acid;  o = carbon dioxide)
 At this point all phenol has reacted with ozone. In
 consecutive reactions glyoxal is converted to glyoxylic
 acid, forming oxalic acid. In the absence of H2°2 oxalic
 acid proves to be a stable final product. Formic acid  is
 oxidized to C0~. The concentration of the organic compounds
               <&*
 formed by secondary reactions is presented in Fig. 4.  At
 the end of the reaction, at an ozone consumption of
 70
10
         -3
           moles, 75 % of all carbon atoms are found as
 C0? and 25 % in the form of oxalic acid.

 It can therefore be concluded that when ozonizing phenol,
 3O % will be hydroxylated to catechol and hydroquinone,
 while 70 % of the phenol will produce unstable peroxides
 which eliminate H2O2 or rearrange to glyoxal, formic  acid,
 or CO,,. CO- and oxalic acid are the only final products.
      <*£*    ^
 The complete ozonization of phenol is shown  in scheme 1.

-------
                           - 371  -
    20.0
     16,0
     12,0
  o
  u
     8.0


     4,0

       0
         0   10  20   30  40  50  60
               ••i   A  03 .lo"*(moles)
70  80
 Fig.  4   The concentration of glyoxylic acid and oxalic acid
          as a function of the ozone-uptake
          (x = glyoxylic acid; . o  =  oxalic acid)
                   3 0
                               35 %


2



H
\ - C
I
HC - 0 -OH
/
HO
2HCOOH ' H,0, + C


4



HO
vc«o
I
HC - 0 - OH
/ 1
HO t
•1 HCOOH +~t
^\J i






°2
I fH 1 °3
j o, cH«o 1 3
' i CO
CC2 | 03 C°2
                                         OH
                                    COT   '
              c"-o
              COOH

              |  o3

              (COOH)-
               1  VHZ°2
       QH

      y
       OH
Scheme 1' The ozonization* of phenol

-------
                        -  372  -   '  .

 2.2   The  ozonization of naphthalene
 Many authors  describe the ozonization of naphthalene in
 organic solvents.  Bailey  et al..  (.10-12)  ozonize naphthalene
 in CC1. and methanol. They assume, that there is production
 of a mono-ozonide  in CCl^, while in methanol they identify
 two  cyclic aromatic peroxides, which  prove to be rather
 stable. Sturrock et al.  (13), ozonize  naphthalene in mixtures
 of water  and  acetone. They assume  an.equilibrium between  an
 open and  a cyclic  peroxide.         .,

 From our  own  experiments  we can  conclude that an open
 hydroxy-hydroperoxide is  produced  after  an ozone consumption
 of 2 moles/mole of naphthalene originally present.  This open
 peroxide  proves to be unstable and results in a cyclization
 or an elimination  of ^2°2'  In the  first  case a cyclic peroxide
 (a 1,2-dioxane derivative)  is formed.

The concentration of  this compound is presented in Fig. 5.
It can be seen that the concentration of the  cyclic peroxide
                                                        ™ "3
reaches a maximum after an ozone consumption  of 16  •  1O
moles (2.9 moles of ozone/mole of naphthalene). The highest
concentration found is 2.3  •  1O   M, indicating that  about
45 % of the total naphthalene originally present will
produce this peroxide. For the remaining 55  %  an elimination
of H_O_ takes place,  forming o-phthalaldehyde  as the  organic
    ^* 4-»
product.  Both concentrations are shown in Fig. 6. Again,
the concentrations reach a maximum after an  ozone consumption
of 16 • 1O   moles. The highest concentrations found  are
2.8  • 1O~3 M and 2.7  • 1O~3 M for o-phthalaldehyde and  ^2°2'
respectively. Without an additional ozone supply all  aro-
matic compounds are converted to o-phthalic  acid. The con-
centration of this compound is shown in Fig.  7. It can  be
                                               _3
seen that after an ozone consumption of 32  •  1O   moles
 (5.4 moles/mole of naphthalene originally present) the  con-
                                         -3
centration of o-phthalic acid is 6.5  • 1O    M. This means
that about 9O % of all naphthalene originally present are

-------
                         - 373 -

  converted  to  o-phthalic acid,  which proves to be rather
  stable  against  further  ozonization.  After an extensive
  ozonization,  7O %  of  all carbon  atoms are measured as
  CO-  and 3O %  as oxalic  acid.

  Therefore,  when naphthalene  reacts  with ozone, an open
  peroxide is produced.   45 %  of this peroxide are converted
  to a very  stable cyclic peroxide,  55 % result in an eli-
  mination of H2O2,  yielding o-phthalaldehyde. Both organic
  compounds  form  o-phthalic acid with an additional supply
  of ozone.  The first part of  ozonization of naphthalene
  is illustrated  in  scheme 2.
                 10    15    2O   25
                  A  03 .io"*(moks)
3O
Fig. 5  The concentration of 3,6-dihydroxy-4,5-benzo-1,2-
        dioxane as a function of the ozone-uptake

-------
                          - 374 -
       0
10    15    2O   25

 A  03 ,lo"4(fnoles)
Fig. 6  The  concentration of o-phthalaldehyde and H2O2 as  a
        function of the ozone-uptake
        (x = o-phthalaldehyde;  o  = H2C>2)
       O
1O    15    2O   25
 A  03 . io"'(mo/es)
3O
Fig. 1  The  concentration of o-phthalic acid as a function
        of the  ozone-uptake

-------
                            - 375
                            >* 35 Z
                               H
C- 0
  I
C - 0
 'OH
                                       XOH
                                       V0-OH
7      V
               ;" - 0
               LO - OH
               ""OH
                                 OH
                                "OH
                                            CH-0
                                             OH
Scheme2  The first phase of the ozonization of
          naphthalene
   2.3  The ozonization of phenanthrene
   As for naphthalene, several authors describe  the  ozonization
   of phenanthrene in organic solvents.  Schmitt  et al.  (14)
   ozonize phenanthrene in chloroform and  find .an.iso ozonide.
   Bailey et al.  (15, 16) ozonize phenanthrene in  methanol and
   identify a cyclic peroxide. The  same  mechanism  is given by
   Sturrock et al. (17) for the ozonization  of phenanthrene
   in water-alcohol mixtures.
   Our own experiments indicate the production  of  an open
   hydroxy-hydroperoxide after an ozone  consumption  of
   5 * 1O   moles of ozone  (1 mole/mole  of phenanthrene
   originally present). In  this case the open peroxide

-------
                          - 376 -
 cyclizizes for less than 5  %, and an  almost  complete
 elimination of H^O,^ takes place, forming  2,2 '-diphen-
 aldehyde as the organic product. Both concentrations are
 given in Fig. 8. In this Figure, a maximum concentration
is shown  for diphenaldehyde of 3.6
                                        1O   at  an ozone
 consumption of 5.O  •  1O   moles. An additional ozone
 supply produces a conversion to '.diphenic acid  which proves
 to be fairly stable against ozonization. After an extensive
 ozonization, CO- and  oxalic acid are the only  final products.
 Thus an open peroxide is produced when ozonizing phenanthrene,
 This peroxide is almost completely converted to 2, 2 '-diphen-
 aldehyde and HpO..,. The aldehyde produces diphenic acid when
 reacting with ozone. The first part of the ozonization of
 phenanthrene is shown in scheme 3 .
    5,0
    4.0
 u  3,O
 c
 o
 o
    2.0
    1.O
                                 O-'
                     __o
                       I
                          I
I
                   6   8
                    03 . ic
I
I
                          10   12   14  16
Fig.  8  The concentration of diphenaldehyde and H2O2 as a
        function of the ozone-uptake
        (x = diphenaldehyde;  o = H202)

-------
                           - 377,-
Scheme3  The first phase of the ozonization of
          phenanthrene    , _
                                                     OH
   2.4  Conclusions from the ozonization experiments
        and discussion
   From the above described and additional experiments (1)
   the following conclusions can be drawn:
1
        When ozonizing aromatic compounds H2O2 is always
     produced.
     zation,
                       will be removed by exhaustive ozoni-
        When ozonizing poly-aromatics , very stable organic
        peroxides can be formed if the initial attack of
        ozone produces an aldehyde group and a hydroxy- •
        hydroperoxide group in ortho position at the same
        benzene ring.

        In all other cases when ozonizing poly-aromatics
        dialdehydes and H2O2 are produced almost completely.

        The organic peroxides as well as the dialdehydes are
        converted into carboxylic acids such as o-phthalic
        acid, diphenic acid, oxalic acid, etc., with an addi-
        tional supply of ozone. When ozonizing larger organic

-------
                       - 378 -


     molecules  (such as humic acid), benzene polyearboxylie ,
     acids (such as 1,3,5-benzene tricarboxylic acids) are
     produced. These acids are rather stable against further
     ozonization.

5.   Formic acid, glyoxalic acid and oxalic acid are the
     only aliphatic acids formed when ozonizing phenol,
     naphthalene and phenanthrene. Oxalic acid proves to
     be a stable end-product in the absence of H0O0.
                                                <£* £

Exhaustive ozonization can never be carried out economically,
so that many carboxyllc acids can be present in the ozonated
effluent. These compounds must be removed in a subsequent
purification step such as activated carbon filtration or a
secondary coagulation step. The second part of the paper'
gives some theoretical and experimental results for the
secondary coagulation step.
3.  Removal of carboxylic acids with aluminium salts

In this part of the paper some models will be presented
by which  the removal of carboxylic acids produced by ozoni-
zation can be described. For  a quantitative approach the
following constants must be known:

-  Stability constants for the hydrolysis of the metal
              %*   **
   salt used  (*B-i- 84)-

-  Dissociation constants for the carboxylic acid  (K1-K ).

-  Stability constants of the produced metal carboxylate
   complexes(P--p ), and in case of soluble complexes:
                                                  5*
-  The solubility product of  the metal hydroxyde  ( KqO).

-------
                       - 379 -

Based on  differences  in  these properties,  the  following
mechanisms of removal may occur:

1.   Formation of  a soluble, strong, negative  complex. The
     removal of  this  complex takes place by  adsor-ption of
     the  complex on a floe of metal hydroxyde. The pH is a
     function of the  metal salt concentration  and varies
     between the pH for  optimum complexation and the iso-
     electrical  point.

2.   Formation of  a soluble, weak complex. The removal is
     caused by adsorption of free carboxylate  ions on a
     floe of metal hydroxyde. The optimum  pH coincides with
     the  iso-electrical  point.

3.   Formation of  an  insoluble complex, which  is removed
     as such. The  optimum pH coincides with  the pH for
     optimum complexation.

With all carboxylic acids and with aluminium salts,as well
as with ferric salts,  the same calculations can be used.
In this paper, the complete calculation will only be given
for the removal of oxalic acid with aluminium salts. The
experimental part will describe the removal of oxalic acid
as well as the removal of o-phthalie acid and 1,3,5-benzene
tricarboxylic acid. -
3.1  The hydrolysis of aluminium salts

Aluminium ions are hydrated in strong acid solutions with
6 coordinatively bound water molecules. When the pH rises
the following steps of hydrolysis take place:
  Al (H20)53+

-------
                       - 380 -
            2+
[A1(H20)5OH]      "*+  H2Q' (   ' > [A1(H20)4(OH)2]   + H30

[A1(H204(OH)2] +     +  H20 - - >• [A1(H20)3(OH)3]   +H30+

[A1(H20)3(OH)3]     +  H20 - - * [Al (H2O) 2 (OH) 4] ~  +H30+

According to Sillen and Kartell (18,1'9) these reactions
have the following  stability constants :
                                    =
                                  3   I '
According to Schwarzenbach (2O)  an "Aj (QH) can be defined
This «ai (OH\ gives "the  ratio of the sura of the concentra-
tions of all metal ions over the concentration of Al    :
 aAl(OH)'
          IA1 -J               [A13+]
                                                                (1)
This formula is  valid when no polynuclear complexes are
produced. This can  be assumed as will be shown in a fol-
lowing paper  (21).  Introducing the stability constants
gives :

         -  1   10~4'89   10~9'37   IP"15'04   10-20,30
a Al(OH) "  1 +  £H+,     + rH+.j2   + rH+-,3    + rfl+, 4   '

The log «.., /QH\  as  a  function of the pH is given in
Figure 9.
 3.2  The dissociation of oxalic acid

 Oxalic acid dissociates in two steps  (22)

-------
                          -  381  -
                  HOx -
HOx
            _.. OX
                                                i
                                            ["° I
                                        [HOx ]

                                                            _5
  As with the hydrolysis of  aluminium salts an a  , .
  can be defined
           [Ox1]    [H2Ox] +  [H0x~]  + [Ox  ]
  Ox(H)
           2—
                           2-
                                                                (5)
Introducing the dissociation constants of oxalic acid
gives
               [H30+][  [H30+]2
 &,
    Ox(H)
              K,
                        K1K2
  Log ao  , .  calculated with  formula (6)  is reprensented
  in Figure 1O.
   16
   12
x
9   8
O
Ol
O
    O
                             Fig.  9
                             Log a ' -,
                             of the pH
                                           as a function
     O
                    8
               PH
                                                                (6)

-------
                       - 382 -
                              Fig. 1O
                              Log aQ
                              of the pH
                                          as  a function
3.3 The complexation  of the aluminium-ion with
    oxalate-ions

The aluminium-ion forms,with oxalate-ions, the following
complexes  (1,23)  :
                  Al(Ox)
    3+
  A1
  A13+ + 30x2~-
                                          -  2
                                             = 107'26
                                                 13,0
                                             - 10

                                [Al(Ox)
                                  3+
                                          - =
                                         2-3
                                [A1][OX-]
                                             =  10
                                                 I6'B
                                             can be de-
(7)


(B)

(9)
For this complexation  the  following a,-, /Ox\
fined J

            [Al1 J     [Al3+]+[Al(Ox)+]+[Al(Ox)2^]+[Al(Ox)33~]
 aAl(Ox) =     ^~ =                 ^(1
                                                               0)

-------
                       -T 383 -  .

Introducing the  stability constants 3 in formula, (1O). ,.,
gives :
 *Al(Ox)
2-.
                                 -,2
With, the help of  formula (5)  formula (11) can be written
as :
                               '2         '3
                          B2[0x']     B3[0x']
  Al (Ox)
                 aOx(H)    aOx(H)
                  aOx(H)
                                                             (12)
So aA-, /o x can be  calculated as a function of the pH when
the oxalic acid  concentration is known. The aa,/n x for
                                 -3    -4     A.Myx;
oxalic acid concentrations of 1O  / 10   and 1O'-  M is
given in Figure  11.
          aai tr>  \  as  a function of the pH for three
           r\_L \ vJX )
      oxalate concentrations;
                                   -4,
      b:  [Ox']=  10   M;  c:[px'j = 1O  M;  d:  [px' ] =  1O  M

-------
                       - 384 -
            tot
Besides an a-,  can be defined  covering all aluminium com-
pounds :
          .M.               £, u -—- \ —  ' in •*     can be defined:
 «A1(OX)
With formula  (1)  and (1O)  formula (15) can be converted
to  :
                               _1
            aAl(OH)    aAl(Ox)     ' _  aAl
                                         -                (16)
  Al(Ox)        «A1(OH)
Al(OH)
or
 log a1       _  ,„„ tot      ,  „
      Al(Ox)  ~  10gaAl   -   l0^ aAl(OH)

-------
                         - 385 -
  Log aA1(o  x can be calculated'as  a  function  of  the  pH
 when the total concentration of oxalic  acid  is  known.
 For oxalic acid concentrations of 10   ,10   and 10   M
  log a'      is represented  in. Figure  12.,
      Al(Ox)
x
o
                   as a. function of the pH for three
       oxalate concentrations;
       b:  [Qx']= 10  M;  c:[0x']= 1O  M;
l=  1O~3M
  3.4  The  precipitation of aluminium hydroxide
 When  the  produced complexes between aluminium and the
 carboxylic  acids  are  soluble in water,  as is the case
 with  oxalic acid, the precipitation of  aluminium, hydro-
 xyde  plays  an important part.  The precipitation of the
 hydroxyde can be  represented by the following simple
 reaction  :

-------
                           - 386 -
  A1
        3+
               3 ' OH"
                         Al(OH)
                                    3, S
    For this reaction the following dissociation  constant
    is known (18)  :
          3+
                    -3
                         -34
                          J4t
KQO =  [ArH [OH"] J  =  10~J*   or    K
      SQ
                                     SO
                                                      = 10
8
                                                           (18)
Together with formula  (13)  this  can be written as

          [Al'j
  K^ =                   "~a
       SO   T   +•, 3 tot
            |_H3O J  a,.
    Formula (19)  gives the limiting aluminium  concentration
    for hydroxyde precipitation as a function  of  the  pH and
    the oxalic acid concentration. The results are  represen-
    ted in Figure 13.
   S
                                 10
Fig.  13  Log  [Al1]  for minimum hydroxyde precipitation as a
         function of  the  pH  with the following oxalate
         concentrations;
                                      -5,
         a:  [Ox']= O M;   b:  [Ox']= 1O  M;   c: [Ox-']= 10
                                                    ,-4
                     -3,
         d:  [Ox']=  1,0   M
                                                               (19)

-------
                        - 387 -
3.5  Discussion and presentation of the mechanisms for
     removal

From paragraphs 3.1 to 3.4 and from Figs, 9 to 13 the
following conclusions can be drawn:

-  At a pH higher than 4 aluminium salts hydrolyse to
   aluminium hydroxyl complexes.

-  At a pH higher than 5,5 oxalic acid is completely
   dissociated into oxalate ions.

-  Independent of the concentration of oxalic acid
   a', ,Q »  reaches a maximum at a pH of about 4.5.
   So at a pH of 4.5 the complexation dominates over
   the hydrolysis as much as possible. The value of
   aAlfo )  varies between 2.5 and 7.1 indicating that
   aluminium ions produce strong complexes with oxalate
   ions.
-  The minimum aluminium concentration for precipitation
   of Al
   acid.
of A1(OH)2 is a function of the concentration of oxalic
-  Besides, it is known that aluminium oxalate complexes
   are soluble in water.

From these facts it can be concluded that oxalic acid is
removed via mechanism 1.  It forms soluble, negatively
charged strong complexes at an optimum pH of 4.5.  At this
pH optimum removal takes place when enough aluminium
hydroxyde is precipitated. At low concentrations of alu-
minium this will not be the case, so the optimum pH for
removal will shift to higher values.

-------
                        - 388 -
Based on the same kind of calculations it can be concluded
that o-phthalic acid is removed by way of mechanism 2.
It gives very weak complexes, which are soluble in water..
The removal takes place by adsorption of free phthalate-
4-ons on a floe of A1(OH)3. 1 ,3,5-benzene tricarboxylic acid
is removed via mechanism 3.  It forms rather weak complexes,
which are insoluble in water. The removal will take place
by precipitation of the complex.

The proposed mechanisms for removal will be tested in the
experimental part.
3.6  Experiments

To check the proposed mechanisms for removal, experiments
have been carried out with a constant concentration of
                           —4
carboxylic acid of about 1O   M and a varying pH and alu-
minium dose. The results for oxalic acid are presented in
Fig. 14. From this figure it can be concluded that the opti--
mum pH for the oxalic acid removal is a function of the
aluminium-salt concentration. At an aluminium-salt concen-
             -4                    "
tration of 1O   M the optimum pH is 6.8.  This pH coincides
with the iso-electrical point of Al(OH)3. At higher alu-
minium-salt concentrations the optimum pH drops to lower
values, approaching the pH where «,-,,_. >  reaches its maximum.
At very high concentrations of aluminium (5 x 1O   M)  the
oxalic acid removal reaches a maximum at a pH of 4.5,  so the
system aims at maximum complexation of the aluminium ion by
oxalic acid. Always an aluminium hydroxide precipitate must
be present to obtain a removal completely in agreement with
the proposed mechanism.

-------
                          - 389 -
      1OO
   O
   o
     O 40 -
Fig.  14  The relative removal of oxalic acid as a function
         of the pH and the concentration of aluminium salt;
            [Al']= 10  M;  0:[A1']= 2.Ox1O  M;  x:[Al']= 4.1x10  Mf
                := 6.2x1O~4M
   The experiments with o-phthalic acid are presented in
   Fig. 15.  From this figure it can be concluded that the
   optimum pH for removal is about 6.4.  This indicated that
   o-phthalic acid is removed" by adsorption of free ions on
   a flox of aluminium hydroxyde. The complexation does not
   play an important part in this case.

   Finally, the experiments, with 1,3,5-benzene tricarboxylic
   acid are presented in Fig. -16.: This figure shows an opti-
   mum removal at a pH of about 4.7, independent of the con-
   centration of aluminium salt." This indicates a removal via
   an insoluble complex.
   Concluding  it can be  said  that  dicarboxylic  acid,  pro-
   duced during ozonization,  can be  removed  in  a secondary

-------
                          - 390,-'


   coagulation step. The acids are removed via.the proposed
   mechanisms. The mechanism for removal can be predicted
   when the strength and the solubility in water of the
   produced complexes are known.
       1001-
Fig. 15  The relative removal of o-phthalic  acid  as  a function
         of the pH and the concentration  of  aluminium salt?
>  1.8x10 4M;
         -4,
                               O:,
         x:  [Al']= 9.3x10  M;
4.4xlO~4M;
      -3,
                  CAl"If= 1.8x1O  M

-------
                           - 391 -
    '1QQ
     eo -
  O  6O
 U'
  fy^ 40
 -° ai|
 ul I—I
 »-
 ms
     20
       23456789  10          ;
       	— pH

Fig. 16  The relative removal of.1,3,5-benzenetricarboxylic
         acid as a function of the pH and the concentration
         of aluminium salt;
         x:
= 1.8x10  M;
= 8.9x1O~4M
                               0:
.'1= 4.5xlO~4M;
   Summary

   In this paper the reaction" of ozone with phenol, naphtha-
   lene and phenanthrene is reported. Mainly the production
   of stable peroxides and carboxylic acids has been investi-
   gated. When ozonizing phenol and phenanthrene only hydrogen
   peroxide is produced. The ozonization of naphthalene  shows
   the production of hydrogen peroxide as well as an organic
   peroxide. This peroxide (a 1,2-dioxane derivate) is very
   stable in the absence of ozone. Additional supply of  ozone
   to all reaction mixtures will result in the production of
   carboxylic acids, such as oxalic acid, phthalic acid,
   diphenic acid, etc.
   The second part of the paper deals with  the  removal  of

-------
                       - 392 -  •


carboxylic acids by coagulation. Some models are pre-
sented by which the removal can take place. Oxalic acid
is removed via adsorption of aluminium oxalate complexes
on a floe of aluminium hydroxyde; phthalic acid is removed
by adsorption of free phthalate ions on a floe of aluminium
hydroxyde, and benzene tricarboxylic acid is removed as an
insoluble complex.
  (1) K.RUITEGF, J.C.
     Thesis Delft  (1978)

  (2) MEYERS, A. P.
     Quarterly reports  KIWA/RIWA Research (1972-1975), 1-11

  (3) v.d. LEER,  R.C., v.d.  MEENT/W.
     Quarterly Reports  KIWA/RIWA Research (1975-1976), 12-14

  (4) BAUCH, H.,  BURCHARD,  H.,  A.RSOVIC, H.M.
     Gesundh. Ing.  9. (197O),  258

  (5) EISENHAUER, H.R.
     J. WPCF 4O  (1968),  1887

  (6) EISENHAUER, H.R.
     J. WPCF 43  (1971),  2OO

  (7) EISENHAUER, H.R.
     Water Res.  _5  (1971),  467

  (8) GOULD, J.P.
     Thesis Michigan (1975)

  (9) GOULD, J.P., WEBER, W.J.  jr.
     J. WPCP 48  (1976) ,  47

 (10) BAILEY, P.S.,  GARCIA-SHAPR, P.J.
     J. Org. Chem.  22 (1957),  1OO8

 (11) BAILEY, P.S.,  BATH, S.S.,  DOBINSON, F., GARCIA-SHARP, F.J.,
     JOHNSON, C.D.
     J. Org. Chem.  29 (1964),  697

-------
                        - 393 - .
(12)  JOHNSON, C.D., BAILEY, P.S".
     J.  Org.  Chem. 29. (1964)-, 7O9

(13)  STURROCK, M.G., GRAVY, B.'j'. , WING, V.A.                :
     Can.  J.  of Chem. 49  (1971-), 3O4

(14)  SCHMITT, W.J., fiORlCONt, E,J., O'CONNOR,  W.F.
     J.  Am.  Chem. Soc. 77  (1955), 564O

(15)  BAILEY,  P.S.
     J.  Am.  Chem. Soc. 78  (1956), 381

(16)  BAILEY,  P.S., MAINTHIA, S.B.
     J.  Org.  Chem. 21 (1956), 1335


 (17) STURROCK,  M.G.., CLINE,  E.L., ROBINSON, K.R.
     J. Qrq.  Chem.  2_8  (1963),  2340

 (18) SILLEN,  L.G.,  MARTALL,  A.E.-
     Stability  Constants
     Special  Publication No.  17, Chem. Soc. London  (1964)

 (19) SILLEN,  L.G.,.MARTELL,  A.E.  '
     Stability  Constants, Suppl. No.  1, Special Publication
     No.  25,  Chem.  Soc.  London  (1972)

 (2O) SCHWARZENBACH,  G.,  HELLER, J.
     Helv. Chim.  Acta 3_£- (1 951) , 576

 (21) HEERTJES,  P.M., KRUITHOF,  J.'C,
     To be published

 (22) WEAST,  R.C.
     Handbook of Chem.  and* Phys.,  52th ed., The Chem.
     Rubber  Co.,  Cleveland,  Ohio (1971/1972)

 (23) SECCO, F.,  VENTURINI, M.
     J. Inorg.  Chem.  14  (1975), 1978

-------
                        - 394  -
MICROFLOCCULATION BY OZONE
D. Maier
1.  Introduction
Although there is no doubt that ozone is neither a floccu-
lating agent nor a precipitating agent in the conventional
sense, but first and foremost an oxidizing agent, repeated
mention is made in connection with ozonization of so-called
microflocculation. All•observations made up to now, given
in a following chapter, show that the ozonized organic con-
stituents of water acquire a special significance in the
explanation of this phenomenon. For this reason, the known
effects of ozone treatment on natural, organic water con-
stituents will therefore be summarized.

2.  Action of ozone on the organic constituents of water
In practically all publications relating to actual prac-
tice/ purely phenomenological reports are found on the
reduction of the water's colour, odour, and taste — an effect
that can be directly perceived by the senses without re-
sorting to measuring apparatus and analytical procedures.
At least the alterations in colour due to the ozone/can be
evaluated quantitatively by a simple photometric process,
and in the case of strongly coloured waters this can be
done directly in the visible spectrum.

By extending the spectrum to the UV-absorption region
further clear changes of the UV-spectra are observed, which
in all cases studied so far show considerably lower ex-
tinction values than for non-ozonized water.

If the dissolved organic carbon, the content of which is
hardly decreased by ozone treatment in the case of waters
charged with natural constituents, is determined at the same

-------
                         - 395 -
time, the first guiding points become clear: in the first
place ozonization does not bring about a quantitative oxi-
dation of the water constituents to carbon dioxide but rather
a chemical conversion of these constituents.

From a comparison of the chemical oxygen demand/ measured
before and after ozonization of organically loaded waters
it is found in addition that this chemical conversion is
above all a conversion into polar constituents rich in
oxygen.  Simultaneous investigations of the molecular-
weight distribution show that in ozonization this chemical
conversion is accompanied by a reduction of the molecular
weight.

Consideration of the reaction mechanisms cited in the liter-
ature  (1,2) makes it evident that, depending on the pH,
both electrophilic additions to any multiple bonds presen^
and radical attacks on the molecule are possible. For
example, it can be clearly shown by infra-red spectroscopy
of isolated ozonized water constituents (3) that ozonization
leads to a strong attenuation of the double-bond character
and the aromatic character, while simultaneously an increase
in the contents of hydroxyl, carbonyl, and carboxyl groups
is noticeable by the greater intensity of the corresponding
absorption bands.

Several recently developed methods for the determination
of organic acids (4-6) indicate on the basis of many
experiments with various waters that the carboxyl groups
produced during ozonization hold quantitatively the key
position in the formation of new functional groups.

-------
§
•H
     0.3
                           396 -

-------
                          -  397 -•
  |^ a)Bodensee-Rohwasser aus60m Tiefe
  I  ] b) Bodensee- Rohwasser aus 60m Tiefe nach Ozonung (0.9 mg/l O3)
     (Aktivkohte-Aceton-Extrakte)           '

"KiO,


300
. i •
T [C]
200
1 —

10O


40
1



    1:10
                  80            3O
              Temperaturprogr.-imm ( 2°C/min)
                                            O
                                            O
Fig. 2  Decarboxylation of  organic water constituents
        a)  raw Lake Constance  water.from a depth of 6O m;
        b)  raw Lake Constance  water from a depth of 6O m
            after ozonization(O.9 mg 03/1)
            (activated carbon-acetate extracts)  1  cm2=1.4yg CC>2
 The  decarboxylation spectrum reproduced  in  Fig.  2 shows
 in 'the  higher temperature range, in which intramolecular
 oxidation processes play  a part, that more  active oxygen
 available for reaction  is present in the ozonized than in
 the  non-ozonized material.
 This may also be why  a  shift of the oxidizability to more
 readily oxidizable compounds can be seen with ozonization
 in  the  temperature-programmed wet oxidation of ozonized
 organic water constituents,  as shown  in Fig.  3, which is
 based on the work of  Weindel and Maier  (8).

-------
                          - 39'8 -
45mifl
3J.5tn«
                  Wet oxidation K2Cr2°7
                   'take Constance water
                    Band filtrate without
                    oEone treatment
                    Lake Constance water sand
                    filtrate after ozone
                    treatment with
                    0.9 s oyV
Omm
      5C-
                   25
          Vol -ppm CO? in $2 carrier gas
gig.3   Oxidation  spectra of organic substances  in Lake
         Constance  water.  Influence  of 63 treatment
3.  Selected effects  of ozone-induced type alteration

    of  organic substances on practical treatment  of

    drinking water

    3.1.   Toxicity  and repopulation with bacteria

All the molecular properties altered by ozonization exhibit

clear effects in the  practice of drinking water treatment.

These too should be given.in summarized form.
In the  first place  it should be mentioned that ozonization

does not produce any  material more  toxic than those already

present in the water.

-------
                        -  399 -
The strongest evidence (9) for this is the fact that
sterile water^ treated with various ozone concentrations and
having a content of about 1.2 mg C/l of organic carbonr
tends increasingly towards bacterial regeneration after
the addition of a bacteria-containing water with increasing
ozone concentration. This effect, desirable in the purifi-
cation of waste water and already practised .in some plants,
indicates that ozonization leads to the formation of less
toxic substances better utilizable by the bacteria.  It is
now also known that this so-called bacterial repopulation
is only observed in waters with a sufficiently high content
of organic carbon, according to the literature usually in
excess of O.5 mg C/l.

Maier's investigations (1O)  on the problem whether humic
acids are made toxic by ozone treatment, with Daphnia
pulex as the test organizm,  also show clearly that, at any
rate in the case of the humic acids of Lake Constance water
no toxic substances are produced by ozonization.

    3.2.  Chlorine consumption and haloform formation
For practice at least equally significant is the clearly
demonstrated fact that the chlorine consumption of water
charged with organic material is essentially slowed down by
preliminary treatment with ozone, and consequently during
the subsequent chlorination a smaller amount of organic
chlorine compounds is formed (11).  This can be clearly
observed by investigating the chloroform formation in waters
ozonized to different degrees.   According to the work of
Stieglitz et al. (12), some waters after ozonization have an
increased content of particularly the readily volatile
organic chlorine compounds.   Since the possibility of oxi-
dation of chloride to chlorine by ozone can be ruled out,
this can only mean that organically bound chlorine is already
present in the material at the start, and that the ozoniza-

-------
                        - 4OO •- ••  -


tion transforms it into compounds of lower molecular weight,
i.e. smaller, more volatile, and capable of determination by
gas chromatography.

4.  Observations on microflocculation
    4.1.  Review of the literature
The described change in the type of the organic water
constituents due to ozone is an essential prerequisite for
understanding the microflocculation associated with ozon-
ization.

For example, Gomella (13) has given a comprehensive report
on the use of ozone in Prance, stating that in certain
special cases the flocculation of colloidal organic sub-
stances occurs, e.g. in the case of strongly coloured, humic-
acid-containing waters.

Gomella and Hallopeau  (14) further assume that the floccula-
tion is due to partial destruction of organic macro-protective
molecules of natural origin, and conclude that this effect
could help to reduce the amounts of conventional flocculation
agents used. Taylor (15) also believes that by  treatment
with ozone the organic constituents of water are changed in
such a way that during the subsequent flocculation with iron
or aluminium salts the quantity of the flocculating agent
required could be reduced.

In a detailed paper on the removal of iron from ground water,
on the other hand, Cromley and O'Connor (16)  describe the
gelling action of ozonized organic substances, which prevents
the flocculation of iron if the dissolved organic substances
have not been largely decomposed.

In the treatment of the water of Lake Constance to supply the
town of St. Gallen with drinking water, Grombach (17) regards

-------
                        - 401 -
it essential to follow the ozonization by a filtration stage,
since "during oxidation a slight turbidity is produced, due
to the precipitation of dissolved material".

The findings of Campbell and Pescod are also of interest  (18);
after ozonization of micro-filtered Scottish lake water,  these
authors observed the formation of an organic foam on the
water surface, the amount of this foam increasing with
increasing colour of the water, i.e. with increasing content
of humic acids.

In studies on the removal of bacteria from a severely polluted
stream with ozone, Franz and Gagnaux (19) observed a marked
after-turbidity in" the ozone-gasification chamber, which
exerted an unfavourable effect on the removal of the bacteria.

The authors assume that the bacteria become encapsulated  in
the precipitated microflocculated material and so avoid being
attacked further by the ozone.

Rohrer (20) claims that any peptides present in the water are
flocculated by ozone, and that clay minerals are also rendered
flocculatable to a large extent by an ozone treatment.

In a treatment of surface water described in a French Patent
(21), the so-called M.D. process/ after micro-filtration ozon-
ization is used to bring about micelle formation, i.e. the
occurrence of organic turbidity, which is removed by "demi-
cellization", i.e. by flocculation with aluminium sulfate in
a subsequent sand filtration stage.

From a spring water containing humic substances Kopecky (22)
isolated, after treatment with ozone, membrane-filterable
organic compounds which he described as "ozonides" and which

-------
                       -  402 -
were shown to be bactericidal after thei,r application to
culture media inoculated with bacteria.

Rempel and Summerville  (23) also observed increased turbidity
after the ozonization of a surface water.

While this literature survey is essentially limited to the
cases in which increased turbidity of a predominantly organic
nature appeared after ozonization, there are also certain
indications that turbidity present initially in water can be
reduced by treatment with ozone.

Sontheimer (24)  was the first to show, in fundamental experi-
ments on the ozonization of Lake Constance water, a clear
reduction of the particle count on the addition of ozone,
which he attributed to an agglomeration of the smallest
particles into micro-flocks.

"Ozone has a precipitating action" claims Kulcsar-Mescery  (25),
mentioning at the same time that fine clay suspensions, iron,
manganese, humic substances, and other colloidal impurities
are precipitated.

O1Donovan (26) studied the use of ozone in three Irish lake
waters, and in the analysis of the ozone-treated waters
established that their turbidity is normally lower than that
of raw water.

In investigations on the filtration of suspended matter from
Lake Constance water, Wagner, Keller and Miiller  (27) found
that the suspended matter can be more efficiently removed by
preliminary ozonization of the water, independently of the
turbidity of the raw water; in the dosage range studied,
between 0.5 and 2.3 g ozone/m ,the amount of ozone used had no
effect on 'the result.   With the simultaneous use of floccu-
lation agents (aluminium sulphate) in these experiments it

-------
                        ,_ 403 -
could also be demonstrated that to achieve the same degree
of removal of the suspended matter -the ozonized water required
less of the flocculation agent than non-ozonized water.  The
authors came to the conclusion "that as a result'of ozon-
ization an improvement in the flocculation and filtration
capacity of colloidal substances is obtained".  This may be
due to the flocculation effect that occurs in the ozonization
of humic-type organic substances.

Similar findings were reported by Wurster and Werner  (28),
who in the treatment of Danube water observed an optimal
removal of very fine suspended particles by means of rapid
filtration with the combined use of secondary flocculation
and ozonization.

The literature evaluation of this problem was conducted as
extensively as possible.  It is already becoming evident
that, according to the described experience gained in the
practice of drinking water treatment, more than one
mechanism can be made responsible for the so-called micro-
flocculation, as indicated by the following experimental
results  -  both our own and those  selected from the liter-
ature.

4.2.     Selected experimental results on micro-flocpulation
In the ozone treatment of micro-filtered water of Lake
Constance a situation is observed at certain times of the
year  -  mostly in the summer during intensive production of
algae and a perceptible increase in the organic products of
algal metabolism  —  that can be characterized purely optically
by an increased foam formation on the water surface in a
reaction tank after the ozonization stage.

This situation, shown in Fig.  4,  can be explained by flota-
tion processes of suspended matter newly formed during the
ozonization.

-------
                         - 404
                                      Fig.  4
                                      Foam formation after
                                      ozonization of
                                      Lake Constance water
TABLE 1  Analysis of the "flotation foam" after ozonization
         of Lake Constance water on Nov. 28, 1977
I'arawct or
Organic substance- as loss on ignition
?oo*c/(ioon*c)
Acid insolublu components as SJO,
Calcium
Kjynesiura
Aluminium
Iron
Manganese
Phosphorus as P0^~
Zinc
Total trace eleme-iits Wg, Se, As,
Pb, Cd, Cr, Co, Ki)
Carbonate, calculated from Ca
Sulphate
Nitrate
Chloride
Total
Percentage proportion
of dry, foan !
40* / («)
29
7.9
3 .1
6.5
2.1
0.5
0.6
0.2
0.04
11.9
not detectable
not detectable
not detect^xb \f?
99.8 ' "

-------
                          -  4O5  -
 Analysis of this  foam,  given in Table  1,  shows clearly that
 the main component  is  organic and that the  original
 supposition of a  high  proportion of calcium carbonate does
 not correspond to the  observed facts.

Table  1  shows^ in addition, a  considerable proportion of
silica,  presumably due to the  shells of smaller  silicic
algae  passing through the microfilter.

An  additional check on the water  body after every  stage of
the treatment of the Lake Constance Water Supply Association
shows, with the aid of Fig.  5,  that the freeze-dried filter-
able solids (TR),  by the method described by Geller (29),
 increase by about 20% after  direct contact  with  ozone, and in
 the next downstream intermediate  tank are concentrated on the
 surface  in addition to the enrichment already  mentioned.
   TR mg/l
   0.5-r
   0,4--
Ca mg I
  0.05
    Raw water      after   after O3-   after O3-   pure water
               micro-  aa^hing    reaction   after sand
             filtration chamber    container   filter
                    (6.9 g 03/m3)
    TR'filterable dry solids.
— ——Ca- calcium fraction of TR
  Fig. 5  "Microflocculation" in the treatment  of Lake Constance
           water
The  proportion of calcium,determined from the  filterable
solids, corresponds in order, of magnitude to the  values
obtained in Table 1; a  stoichiometric check shows  that this
is not precipitation of  calcium carbonate.

-------
                          - 406 -
What is much more likely  is  that  a  small  proportion of the
organic acids formed after ozonization  is precipitated in the
form of calcium  salts.

These first observations  on  micro-flocculation suggest that
the above situation, which often  occurs in lake waters and
has so far been unexplained,  is somehow   connected with the
phytoplankton content of  the water  and  its metabolic products,
The following figure indicates  that  the  metabolic products
of the phytoplankton probably play the leading  role.
                       May/June 1976
                    Jan/Feb. 1977
           Ozone dosage (g Oo/m )
 Fig,  jj  Dependence of microflocculation in sand-filtered
         Lake Constance water from a depth of 6O m on the
         type of dissolved organic water constituents
In this experiment both phytoplankton  and  zooplankton had
been eliminated as a start by  intensive  sand  filtration.
Microscopic examination of the starting  water showed an alga-
free filtrate with ^ery good turbidity values of  0.07 form-

-------
                          - '407  -

  azine-turbidity units.  Only in water with a high  initial
  plankton content could larger turbidity increases  due  to
  ozone treatment be observed, while in the water  from full
  circulation of the lake, with a low initial alga content,
  practically no turbidity increase occurred at  all  ozone con-
  centrations.  It must be emphasized that the turbidity values
  cannot be changed by acidification of the water  with nitric
  acid or hydrochloric acid, so that here too the  influence  of
  calcium carbonate can be excluded.

  It can also be seen from Fig.  6 that  in the  summer there  is
  an additional optimum of the ozone concentration,  at which  '•
  these increases in turbidity occur.

  If we start out from a water rich in  inorganic turbidity  but
  low in plankton, then, as Fig. 7 shows, the  initial turbidity
  decreases with increasing dose of the ozone  up tq  a concen-
  tration of about 1.5 mg 0-,/l.
  oso-
_p
•H
tf
•H
f,
tt
  0.35
                    Ozone reaction time 1.25 h
                       Experimental series 25.2.77
       Exper iment al
       series 1.3.77
                       Ozone dose (mg 03/1)
                    1.0
   Fig. 7  Change in the raw water turbidity  in  dependence -on
           the ozone concentration

-------
                         -  408  -
Similar  results were obtained by  Schalekamp (30} , who reported
on the example of Lake Zurich water  that ozone reduced the
turbidity by about 20 - 40%, depending on the amount used.
To gain  a better understanding of the 'flocculating action of
ozone, in the case of these Zurich investigations the part-1
icles were counted by size classes before and after the
ozonization.
 Rwrtiole
 frequency
               before ozonizatioa
               Total of particles 13. 040/ral
               after ozonizatioa
               Total of particles 8158/ml
   0,7 1,0
                                     Fig.  8
                                     Microflocculation in the
                                     treatment of Lake Zurich
                                     water (after M. Schalekamp)
          Rarticle siZB 0
As can be  seen from Fig. 8, the smaller  particles are reduced
after ozonization with 1 mg/1, while  the larger particles
increase.   Before the ozonization the total number of all
particles  with a mean diameter of 2.35 ym was 13,040 and
after  ozonization, with a  larger  mean particle diameter of
2.97  pm,  it was only 8158.   In  ozonization with  2.5 mg 0^/1
the  same phenomenon can be detected,  but much more weakly.

-------
                          - 409 -    _   .,,


Still higher  ozone concentrations invert  the  picture,  so that
after ozonization more particles are present  than before.

These examples/ cited from the practice  of drink'ing water
treatment make  it clear that two opposing processes are at
work here, which make the interpretation  of the phenomenon
of micro-flocculation considerably more difficult.

The input of  energy into the water must also  be regarded as
a factor influencing the formation of micro-flocculation.
With varied conditions of operation of  a  container into which
the ozonized  water, is introduced for a  secondary reaction,
by changing the proportions of the potential  energy E = g-h
                               2
and kinetic energy E = 1/2 m-v , the remaining water prop-
erties remaining constant, a manipulatable change in turbid-
ity can be produced, as shown in Fig. 9.
 6-i
E 5-
  0-
                   2.2

                  -2.0"
                    C
                  -1.8*
                                         •1.2-fi
                                        1-1.0
     Amount of raw water
        required
Throughput through intermediate
 container QSt
    16   18
    2? 2 75
             2O '  22  •  24
                                       i
                                       8 hours
 Fig.  9  Microflocculation  in  ozonized Lake Constance water

-------
                      •  - 410 - -

Since in this example too no precipitate of calcium carbonate
can be detected analytically, which could be easily accounted
for by the different turbulence within the container, it is
certain that the turbidity changes observed are due solely
to the superimposition of two opposing processes and their
dependence on the energy input.

4.3.     Results from model experiments
There is no doubt that in the interpretation of experimental
results of this kind in practice which, as already mentioned,
also depend on the season and on the general limnological
state of the waters, a somewhat unsatisfactory feeling almost
of uncertainty is obtained.  It therefore is absolutely
necessary to confirm the results relating to actual practice
by aimed model experiments.  The main difficulty in an effort
of this kind lies in achieving comparability of the experi-
mental conditions between the practice trial and the model
experiment, which is of special significance in the case of
the organic water constituents.

We now know that it is not possible to characterize the
natural organic constituents of a surface water so precisely
that a synthetic aqueous solution of these could be made up.
Therefore, the preparation of model solutions is always
directed at the enrichment and isolation of these substances
which during the enrichment phase are changed by chemical
reactions with one another or by redox processes in such a
way that the isolated substance is no longer identical with
the substance originally present (7).

This fact alone explains why until now it has only been
possible in a few cases to achieve by means of ozone treat-
ment a perceptible increase in turbidity on a model water
enriched with 30 mg/1 of hymatomelanic acid, and hence a
conversion of the dissolved substances into the colloidal
state, as shown in Fig, 10.

-------
                           - 411  -
                             30mg/l UMA
                           without, CaCU-adc*i't:ioj:l
             0,1    0,5
              Degree of ozoBissation'fmgOj/mg  acidj
Fig.  10  Microflocculation of hymatomelanic acid  (HMA)  by O.
          (after R. Kurz)        -
The opposite effect, i.e.  a reduction of "the turbidity of  a
particle-rich water enriched with huraic acid by ozone treat
ment, has  been repeatedly  demonstrated, as  Kurz's results
with kaolin suspensions  (31)  show  in Fig.  11.

-------
                         - 412 -
                  KXJmg/l  K*o)in
                  Smmof/i  C»CI2
      o       0.2       0.4      o.e      as
    specific ozone dosage  (mg 03/mg HA)
Fig. 11
Destabilization of a
kaolin suspension by
Lake Constance humic
acids  (HA) after
ozone treatment
To explain these relationships in the two model trials,
differing from the relationships observed in practice, we
can only assume that in the formation of filterable substances
which increase the turbidity  (case 1) from dissolved substances,
in the first place the entire molecule structure plays the
decisive part, while the turbidity-diminishing action  (case  2)
depends mainly on the number and type of the functional groups
and less on the basic skeleton of the carrier molecule.  To
the first case is added the possibility of the formation of
insoluble alkaline earth metal salts, which is also structure-
dependent.  Summarizing case 1, it must be emphasized that
in contrast to true flocculation, these processes  should be
regarded rather as precipitation reactions.
Case 2 can be explained by the ozone action on organic water
constituents described in the first chapter: non-ozonized
humic acids are adsorbed on the mineral particles forming  the
turbidity and endow them with an increased suspension capac-
ity by a kind of protective-colloid action.  If the polarity
of the organic water constituents is increased by the ozon-
ization, which manifests itself in a considerable increase

-------
                        - 413 -
in the number of carboxyl groups, while on the other hand
the size of the molecule is decreased, the adsorption
capacity on the turbidity particles immediately rises.  At
the same time, however, after ozonization substances are
more strongly adsorbed on the turbidity material which, owing
to their greater number of functional groups, now has essen-
tially greater possibilities for cross-linking by bridge-
formation.  The fact that this "polyelectrolyte character"
of ozonized organic constituents is noticeable, even with a
relatively small dose of  ozone, and in the case of the humic
acids of Lake Constance reaches its optimum with ozone doses
between 0.8 and 1.0 mg/1, indicates that the molecular size
and therefore also steric factors exert an influence that
should not be neglected.

According to Stumm's classification of the agglomeration of
colloidal particles, case 2 comprises true flock formation
in which the destabilization mechanism is designated as  floccu-
lation.

5.  Summary
Ozonization of the organic constituents of water gives rise
to more polar compounds richer in oxygen and poorer in
double bonds, with increased numbers of hydroxyl, carbonyl,
and carboxyl groups and lower molecular weights.  This ozone-
induced change in structure is performed by two simultaneous
and opposing processes.

The first case  -  the production of precipitating products
from originally dissolved organic compounds  -  is probably
strongly dependent on the structure of the entire molecule.
                           v
This includes the precipitation of insoluble alkaline earth
metal salts of organic acids.  Particularly in the case of
lake waters there are indications that special metabolic

-------
                         - 414 -
products of the phytoplankton are made precipitable by
ozonization.  This phenomenon is observed in practice as a
turbidity increase after ozonization.

The opposing process - destabilization of turbid suspensions
by ozonized organic water constituents -r should clearly be
regarded as true flocculation, the ozonized organic substances
adsorbed on the turbidity particles contributing by bridge-
formation to cross-linking of the particles as a result of
their polyelectrolyte character.  A decisive part is played
here not so much by the entire structure of the organic
constituents as by the nature and quantity of their functional
groups.

In water-treatment practice the two processes presumably
take place in opposing directions and, in addition, are depend-
ent on turbulence.  The reported investigations are partly
the results of a research programme on the optimization of
the ozone process, promoted by the Water Economy Board. Some
of the methods of characterizing organic water constituents
were newly developed within the framework of a research
programme, promoted by Dechema, for influencing corrosion
processes with the aid of organic water constituents.

-------
                       -415-
(1) AUTORENKOLLEKTIV
    Organikum, Organisch-Chemisches Grundpraktikum:
    Ozonisierung
    VEB Deutscher Verlag der Wissenschaften, Berlin  (197O),
    295-299

(2) REICHERTER, U.-F.
    Untersuchungen iiber die Anwendung von Ozon bei der
    Wasser- und Abwasserreinigung
    Dissertation, Fakultat fur Chemieingenieurwesen,
    Uniyersitat Karlsruhe  (1973)

(3) MAIER, D.
    Wirkung von Ozon  auf geloste organische Substanzen
    Vom Wasser £3 (1974), 127-16O

(4) EBERLE, S.H., SCHWEER, K.H.
    Bestiramung von Huminsaure und Ligninsulfonsaure im
    Wasser durch Fliissig-Fliissigextraktion
    Vom Wasser 4J_ (1973), 27-44

(5) MAIER, D., FUCHS, F., SONTHEIMER, H.
    Bestimmung von organischen Sauren in Wassern und auf
    Aktivkohle
    gwf-Wasser/Abwasser 117 (1976), 2, 7O-74

(6) WAGNER, I., HOYER, O.
    Die Bestimmung von Huminsauren und Ligninsulfonsauren
    in Wassern mittels UV-Spektroskopie
    Vom Wasser 45 (1975), 2O7-216

(7) MAIER, D.
    Temperaturprogrammierte Decarboxylierung organischer
    Wasserinhaltsstoffe
    Vom Wasser 5O (1978)  (in press)

(8) WEINDEL, W., MAIER, D.
    Das Oxydationsspektrum, eine analytische Hilfe zur
    Beurteilung von Verfahrensschritten der Wasseraufbereitung
    Vom Wasser 5O (1978)  (in press)

(9) MAIER, D., KURZ, R.
    Untersuchungen zur Optimierung der Ozonanwendungibei
    der Aufbereitung von Seewasser
    Veroffentlichungen "Internationales Symposium Ozon und
    Wasser" Wasser Berlin 77 (1977), 211-232


(1O) MAIER,  D.
    Werden Huminsauren durch Ozonbehandlung toxisch?
    Vom Wasser,  5J_  (1979) (in press)

-------
                       - 416 -
(11) MAIER, D., M&CKLE, H.
     Wirkung von Chlor auf natilrliche und ozonte organische
    , Wasserinhaltsstoffe
     Vom Wasser 47 (1976) , 379-397

(12) STIEGLITZ, L., ROTH, W. , KtlHN, W. , LEGER, W,
     Das Verhalten von Organohalogenverbindungen bei der
     Trinkwasseraufbereitung
     Vom Wasser j47_ (1976), 347-377

(13) GOMELLA, C.
     Ozone Practices in France
     J. AWWA £4 (1972), 1, 39-45

(14) GOMELLA, C.,  HALLOPEAU, J.
     Progre"s sur la recherche sur les effects de 1'ozone
     sur 1'eau pendant le traitement et dans le reseau de
     distribution
     Publication 9th IWSA Congress New York  (1972), 20-24

(15) TAYLOR, E.J.
     'Weshalb Philadelphia Ozon nahm
     Translation from "The American City Magazine" (1949)

(16) CROMLEY, J.T., O'CONNOR, J.T.
     Effect of Ozonation on the Removal of Iron From a
     Ground Water
     J. AWWA 68 (1976), 315-319

(17) GROMBACH, P.
     Die neue Ozonanlage des Bodensee-Trinkwasserwerkes
     der Stadt St. Gallen
     Monatsbulletin SVGW 4j| (1968), 7, 211-215

(18) CAMPBELL, R.M.,  PESCOD, M.B.
     The Ozonization  of Turret and Other Scottish Waters
     Water and Sewage Works 113 (1966) , 7, 268-272

(19) FRANZ, J., GAGNAUX, A.
     Entkeimung mit Ozon unter besonders hohen Anforderungen
     Wasser, Luft  und Betrieb 15 (1971), 11, 393-396

(2O) ROHRER, F.
     Ozon und  seine  Verv/endung zur Wasseraufbereitung
     Schweizer Brauerei-Rundschau 63  (1952), 135-155

(21) DIAPER, E.W.J.
     A new method of treatment for surface water supplies
     Water and Sewage Works 117  (197O), 11,  373-378

-------
                       - 417 •--•'••
(22)  KOPECKY,  J.                           '
     Erfahrungen  mit der Ozonisierung
     Fortschritte der Wasserchemie 1_ (1967), 94-10O

(23)  SUMMERVILLE, R.C.,  REMPEL, G.
     Ozone for Supplementary Water Treatment
     J.  AWWA 6_4 (1972) ,  377-382

(24)  SONTHEIMER,  H.   .
     Erfahrungen  beim Einsatz von Ozon in der Trinkwasser-
     aufbereitung;   3. Ozon als Oxidations- und Flockungs-
     hilfsmittel
     Wasserfachliche Aussprachetagung des DVGW/VGW in
     Wiesbaden (1971),  Sonderdruck DVGW-Brosentire
     "Wasseraufbereitung-Wasserzahler"

(25)  KULCSAR-MESCERY, J.
    'Wasseraufbereitung durch Ozonisierung
     Gas,  Wasser, .Warms 9_ (1955), 8,  193-195

(26)  O1DONOVAN, p.C.
     Treatment with  Ozone
     J.  AWWA (1965), 9,  1167-1194

(27)  WAGNER, I.,  KELLER, H., MULLER,  R.
     Untersuchungen  zur Aufbereitung  von Bodenseewasser
     mittels Ozonung und Flockungsfiltration
     gwf-Wasser/Abwasser 118 (1977) ,  320-322

(28)  WURSTER,  E., WERNER, G.
     Die Leipheimer  Versuche zur Aufbereitung von Donauwasser
     gwf-Wasser/Abwasser 112 (1971),  81-9O/193-199

(29)  GELLER, W.
     Zur gravimetrischen Bestimmung des Suspensagehaltes
     von Gewassern
     7.  Bericht der  Arbeitsgemeinschaft Wasserwerke
     Bodensee-Rhein  (1975),  146-151

(3O)  SCHALEKAMP,  M.
     Die Erfahrungen mit Ozon in der  Schweiz, speziell hin-
     sichtlich der Veranderung von hygienisch bedenklichen
     Inhaltsstoffen
     gwf-Wasser/Abwasser 5J7 (1977),  9, 657-673


(31)  .KURZ, R.
     Untersuchungen  zur Wirkung von Ozon auf Flockungs-
     vorgange
     Dissertation, Fakultat fiir Chemieingenieurwesen,
     Universitat  Karlsruhe  (1977)

-------
                       - 418 -

PRACTICAL USES OF OZONE IN DRINKING WATER TREATMENT

E.G. Rice, C.M. Robson, G.W. Miller and A.G. Hill
Abstract

Ozone has been used continuously for the treatment of
drinking water since 19O6 in the city of Nice, France,
where it was first installed for disinfection purposes.
Since 19O6, the uses of ozone for water treatment have
grown to include various chemical oxidation processes,
in addition to bacterial disinfection and viral inacti-
vation. Applications for ozonation now include oxida-
tion of inorganic materials (such as sulfides, nitrites,
cyanides, ferrous and manganous ions), organic materials
(such as phenolics, detergents, pesticides, taste and
odor—causing compounds, color-causing organics/ other
soluble organics), turbidity or suspended solids floccu-
lation  (by changing the surface characteristics)t micro—
flocculation and recently to promote aerobic biological
processes conducted in filter media. Each of these uses
of ozonation is discussed in terms of their chemistries
and extent of application in the more than 1,OOO drin-
king water treatment plants known to be using ozonation,
most of which are located in Europe.
1.   History of Ozone Use in Water Treatment  (1)

The earliest experiments on the use of ozone as a germi-
cide were conducted by de Meritens in 1886 in France,
who showed that even dilute ozonized air will effect the
sterilization of polluted water. A few years later  (1891),
the bactericidal properties of ozone were reported by

-------
                       - 419 --  :

 Fr5hlich from pilot tests conducted at Martinlkenfeld in
 a drinking water treatment plant erected by the German
 firm of Siemens  & Halske.  In 1893, the first drinking
 water treatment  plant to employ ozone was erected at
 Oudshoorn, Holland.  Rhine River water was treate'd with
f
 ozone,  after settling and filtration.  Siemens & Halske
 next built treatment plants  at Wiesbaden (1901)  and
 Paderborn (1902)  in Germany  which employed ozone.

 A group of French doctors studied the Oudshoorn plant
 and its ozonized water and,  after pilot testing at
 St. Maur (in Paris)  and at Lille, a 5 mgd plant was con-
 structed at  Nice, France (the Bon Voyage plant), which
 employed ozone for disinfection. Because ozone has been
 used continuously at Nice since the Bon Voyage -plant be-
 gan operating in 1906, Nice  is referred to as "the birth- '
 place of ozonation for drinking water treatment".

 Full-scale water treatment plants then were constructed
 in several European countries. As of 1916 there were 49
 treatment plants in Europe having a total capacity of
 84 mgd (2) in operation, and 26 of these were in France.
 By 194O the  number of drinking water treatment plants
 throughout the world using ozone had risen to 119 , and
 as of 1977 at least 1O43 plants, mostly in Europe (Table 1)
 are known to be  using ozone  for drinking water treatment
 (3). As might be expected, most of the European drinking
 water treatment  plants using ozone are in France, although
 Switzerland  and  Germany account for most of the remainder
 of the European  plants.
                                    y
 In the United States, only one plant has been using ozone
 continuously since the early 1940s (Whiting, Indiana)(4,5).
 In 1973 the  second U.S. plant to use ozone went on stream
 at Strasburg, Pennsylvania' (6) . The remaining ..three plants
 became operational in late 1977 or early 1978 (7).

-------
                        -  42O -
TABLE  1   Operational plants  using ozone - 1977
Country
France
Switzerland
Germany
Austria
Canada
England
The Netherlands
Bel gi urn
Poland
Spain
USA
Italy
Japan
Denmark
Russia ...
Norway
Sweden
Al geria
Syria
Bulgaria
Mexico
Finland
Hungary
Corsica
Ireland
Czechoslovakia
Singapore
Portugal
Morocco

Number of Plants
593
150
136
42
23*
18
12
9
6
6
5
5
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Total 1039
  Includes expansions.  Actual  number  of operating plants
  in Canada = 20,  with 3 more under  construction.

-------
                        421 . -
The fact that the U.S. _Environmental Protection Agency
recently has proposed regulations for the larger U.S.
water supply systems regarding control of organic che-
micals in U.S. drinking water supplies has prompted a
keen interest on the part of the North American water
supply industry to know more about the uses of ozone and
its engineering parameters than had been known in late
1974. In response to this interest, the U.S. EPA funded
a survey, conducted by Public Technology Inc., of
Washington, B.C., to assess the .state-of-the-art of the
use of ozone and of chlorine dioxide for the treatment
of drinking water. This assessment involved a significant
questionnaire survey of many European drinking water
treatment plants, in which many of you participated. In
May, 1977, the PTI survey team visited some two dozen
European drinking water treatment plants using ozone
and/or chlorine dioxide. In August 1977, the PTI survey
team visited nine plants in the Province of Quebec, Canada,
The present paper is taken from the results of this EPA-
funded study.
2.   Applications of Ozone in Water Treatment

Ozone is a powerful oxidant  (Table 2). In acid solution,
the oxidation potential of ozone  (2.O7 volts) is second
only to that of elemental fluorine among the commonly
used oxidants for drinking water treatment. Because many
contaminants in raw water supplies are oxidizable, ozone
can be and is being used for many different applications.
The major uses for ozone in modern drinking water treat-
ment processes are listed in Table 3. Although the early
uses for ozone in treating drinking waters were predomi-
nantly for disinfection (bacterial kill and viral inac-
tivation), today oxidative applications account for a
significantly increasing number of installations.

-------
                          -  422  -
TABLE 2   Oxidation-reduction potentials of  water treatment agents


F2
°3
H2°2
MnO.
4
HC1O
Mno4
HOC1
ci2
HOBr
°3 +
cio2
Br2
HOI
cio2
cio-
HO2~
cio2-
OBr~
X2 +
I" 4-
oi- -
°2 +
REACTIONS

+ 2e = 2 F~
•f 2H+ + 2 e =0, + H_O
, * J.
•f 2H -f 2e = 2H2O (acid)
- -f 4H+ + 3e = Mn02 -f 2H2O
_ + 3H+ + 4e = Cl~ + 2H_0
+ 74-
- + 8H +5e = Mn + 4H2O
+ H+-i-2e - Cl~ + H20
+ 2e =2 Cl~
•f H+ + 2e =« Br~ + H2O
H2O + 2e = O2 + 2 OH~
(gas) + e = C1O2~
+ 2e = 2Br~
•f K+ + 2e = I~ + H2O
Caq) + e = C1O2~
+ H2O + 2e = Cl~ + 2OH~
-{- H20 + 2e = 3OH~ (basic)
~ + 2H2O 4- 4e = Cl~ + 4OH~
+ H20 + 2e = Br~ + 2OE~ • •
2a = 2 I~
2e = 3 I~
f- H2O + 2e = I" + 2OH~
2H2O + 4e = 4OH-
POTENTIAL IN VOLTS (Ec)
25 °C
2.87
2.07
1.76
1.68
1.57
1.49
1.49
1.36
1.33
1.24
1.15
1.07
0.99
0.95
0.9
0.87
0.78
0.70
0.54
0.53
0.49
0.40
    "Handbook of  Chemistry & Physics,  56fch  Edition, 1975-76.
     Press Inc.,  Cleveland, Ohio, p.  D-141-143.
CRC

-------
                           - 423 -
TABLE 3  Applications of  ozone in drinking water treatment
   Bacterial  Disinfection
   Viral  Inactivation
   Oxidation  of Soluble Iron  and/or Manganese
   Decomplexing Organically - Bound Manganese  (Oxidation)
   Color  Removal (Oxidation)
   Taste  &  Odor Removal (Oxidation)
   Algae  Removal (Oxidation)
   Oxidation  of Organics
       -  Phenols
       -  Detergents
       -Pesticides
   Microf1occulation  of Dissolved Organics  (Oxidation)
   Oxidation  of Inorganics
       -  Cyanides
       - Sulfides
       - Nitrites
   Turbidity  or Suspended Solids Removal  (Oxidation)
   Pretreatment for Biological Processes  (Oxidation)
       - On  Sand
       - On  Anthracite
       - On  Granular Activated Carbon
   To Make  Treated Water Blue
 2.1  Bacterial Disinfection
 The French have pioneered the use of  ozonation for  bac-
 terial  disinfection. Guinvarc'h  (8) reported that ozo-
 nation  at the Paris, France, St. Maur  plant produced wa-
 ters which never showed the presence  of  E.  coli, although
 the raw Marne river waters at the time showed coliform
 counts  of 15,OOO to 1OO,OOO units/liter,  with average
 values  of 3O,OOO to 5O,OOO.  O'Donovan (9)  points out
 that for  bacterial disinfection, the  usual ozone dosage
 rate in water treatment plants  at that time was  I.5 to
 2 mg/1.

-------
                       - 424 -
Miller et al.  (3) have foundtfiat'the'' current average
ozone dosage rates in drinking water  treatment plants
are 1 to 4 mg/1 today. Where preozonation treatment is
non-existent or when loadings of ozone-demanding organic
or inorganic materials are high, such as during pollution
episodes, ozone doses much in excess  of 2 mg/1 can be re-
quired to attain the desired degree of bacterial disin-
fection. On the other hand, only O.25 mg/1 of ozone pro-
vided satisfactory disinfection at Boxley, England  (1O).

In addition, the bactericidal action  of ozone is.little
affected by changes in temperature or pH, and the disin-
fecting action of ozone is virtually  instantaneous. Weaker
oxidants require more contact time  (and usually higher
concentrations) to provide the same degree of bacterial
disinfection  (9).
2.2  Viral Inactivation

In this application the pioneering work was conducted by
French public health officials. Coin and his coworkers
(11,12) used poliomyelitis virus Type I (1964)  and Types
II and III (1967) to demonstrate that when an amount of
residual dissolved ozone equivalent to O.4 mg/1 can be
measured after 4 minutes of continuous ozonation, the
degree of viral inactivation surpasses 99.9 %.

Subsequent to this work in the late 1960s, the city of
Paris adopted the O,4 mg/1 residual ozone after 4 minutes
as a standard for the use of ozonation for viral inacti-
vation. Currently this.standard has been adopted through-
out France (13) ,

-------
                        -  425  -
In actual plant practice, . in. order to,, insure that these
minimum ozonation conditions are met consistently, the
French increase the ozonation time to at least eight mi-
nutes, and sometimes contact times of 12 minutes are.em-
ployed. It is general French practice to use at least
two ozone contacting chambers.. In the first chamber the
initial ozone demand of the water is satisfied and the
residual of O, 4 mg/1 of ozone., is attained; in the second
chamber the O.4 mg/1 of ozone residual is maintained.
Satisfying the ozone demand of the water in the first
contact chamber requires relatively large amounts of
ozone, and some 67 % of the total ozone dosed is applied
here. Lower amounts of ozone  (about 33 %) are applied to
the second chamber to maintain the O.4 mg/1 of dissolved
ozone, usually for periods of 4 to .8 additional minutes.

At the present time, France is the only country known to
have formally adopted these ozonation conditions as a
treatment standard for viral inactivation. However, many
plants in countries outside of France have designed ozo-,
nation contacting systems for disinfection which utilize
the same treatment conditions  (3). It is important to re-
cognize that under these viral inactivation conditions
of ozonation, bacterial disinfection also is obtained.

Ozonation of organic compounds usually produces oxyge-
nated  organic materials which  are more readily biodegra-
dable. In addition, ozonation  does not readily oxidize
ammonia except at high pH  (above 9). For these reasons,
ozonized waters containing these materials generally are
treated with small dosages .of  chlorine, chlorine dioxide
or chloramine to prevent bacterial regrowths in the dis-
tribution networks.

-------
                       - 426 -
In Switzerland, it is common practice in many of the
15O drinking water treatment plants to follow ozonation
with filtration through granular activated carbon in or-
der to deozonize the water, then to add chlorine dioxide.
Deozonation is considered necessary because of reactions
between ozone and ClO^ in aqueous solution  (14).
Masschelein (15) points out that ozonation of solutions
of chlorine dioxide or sodium chlorite in the mg/1 range
produces chlorate stoichiometrically:
         NaClO2 + 03  	> .NaClO3 + O2

The dimerization of C1O2 to C12O4 is considered to be
the first step in the ozonation process  (16). The reac-
tion is very fast and is almost controlled by the diffu-
sion rate of ozone  (17).
Many European, Canadian and the Strasburg, Pennsylvania
plants employ ozonation as the terminal treatment disin-
fection step, however, without aftergrowth problems.
Miller et al. (3) have concluded that ozonation can be
used as a terminal treatment step only if all of the
following conditions are met simultaneously:

1)  The distribution system must be clean and not subject
    to leaks,
2)  Dissolved organic carbon concentrations must be less
    than O.2 mg/1,
3)  Ammonia must be absent,
4)  Water temperature must be low and
5)  Residence time in the distribution must be short
    (less than 1 day).

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                       - 427 -
.2.3  Oxidation of Soluble Iron & Manganese

Ferrous iron is oxidized rapidly by ozone to ferric ions,
which then hydrolyze, coagulate and precipitate according
to the following equations:

               Fe+2 + 03  —»  Fe+3

               Fe+3 + H20  	>  Fe(OH)3

Similarly, manganous ions can be oxidized to manganic
ions, which then form insoluble manganese dioxide:

                 + ?             +4
               Mn   + 03 	>  Mn

               Mn+4 + H20 	>  Mn(OH)4 	>  MnO2

However, over-ozonation of manganous compounds produces
the very water soluble permanganate ion, which is pink
in color:

                 +2      +4               -
               Mn   or Mn   + 0_ 	j>  MnO,
Permanganate is toxic and should be prevented from en-
tering water distribution systems. Its presence in dis-
tribution networks can lead to buildup of MnO2 scales.
Permanganate can be produced  (or used) in the water treat-
ment plant but kept from entering distribution system net-
works by several procedures, all of which depend upon the
fact that it is a very strong oxidant  (see Table 2) with
an oxidation potential of 1.68 volts, close to that of
ozone.

-------
                         428 -
Normally, ozonation of ferrous and manganous - compounds is'1
conducted early in the water treatment process and the
hydrolyzed and precipitated inorganic hydroxides are fil-
tered. If the filtered water is distinctly pink in color,
the operator is alerted to the fact that he is using too
much ozone, and the ozone dosage should be reduced. Tanks
which provide 15 to 3O minutes of holding time are used
in the Dusseldorf area. This allows the permanganate to
oxidize dissolved organic materials, thereby being reduced
to the insoluble manganese dioxide:
             MnO
.   +  organics  	>  MnO2  + oxidized organics
Alternatively, the pink water can be filtered through gra-
nular activated carbon, where the permanganate is quickly
reduced to MnO- in the first few centimeters of the bed or
column. The insoluble dioxide then is removed during rou-
tine backwashing of the activated carbon medium.
2.4  Decomplexing of Organically-Bound Manganese

When iron and manganese are present as free inorganic
cations, they can be oxidized readily by agents much weaker
than ozone —  simple aeration is known to be effective,
for example. However, when manganese is present as orga-
nic complexes, as is the case when decaying vegetation is
present, then even chlorine is not powerful enough to
break down the manganese complexes. In such cases stron-
ger oxidants, such as chlorine dioxide or ozone, are used,
again as a preoxidation step so as to prevent the oxidized
manganese from being passed into the distribution system.

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                       - 429 -


2.5 .Color. Removal    • ••,  .'.-v  ...'••        ••  <•   ••    •'  •

Usually the colors in drinking water are derived from the
decomposition of naturally occurring humic materials. Co-
lors usually are caused by the presence of unsaturated
organic moieties conjugated in the compounds  (i.e., alter-
nating double and single bonds). Compounds containing
such conjugated groupings are referred to as chrbmophores..

Ozone is particularly reactive with unsaturated groups,
cleaving the carbon-carbon double bonds to produce ketones,
aldehydes or acids, depending upon the other substituents
on the carbon atoms affected, the amount of ozone and con-
tact conditions applied. As soon as the conjugation has
been disrupted by oxidation, the color will disappear. This
does not necessarily mean that all of the color-causing
organic compound has been converted to carbon dioxide and
water, however, but simply that the conjugated unsaturated
groups responsible for the original color have been des-
troyed .

In industrial areas where textile manufacturing or dyeing
is prevalent, organic dyestuffs sometimes are discharged
from these industrial installations and are found in raw
waters entering drinking water treatment plants. These
dyestuffs generally are polycyclic, highly conjugated or-
ganic materials, easily decolorized by a powerful oxi-
dizing agent such as ozone. As before, however, the de-
colorized water will still contain considerable dissolved
organic carbon, which is readily biodegradable.

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                       - 430 -

2.6  Taste and Odor Removal

As a general rule, taste and odor causing compounds are
organic in nature, although many inorganic sulfides also
are encountered which are highly odorous. Many of these.
organic compounds which cause unacceptable tastes and
odors are formed from natural vegetation during anaerobic
decomposition in the ground or in surface waters in which
the dissolved oxygen content may -be too low to support
aerobic colonies. Examples of such materials include ter-
pene derivatives, such as geosmine, and some alicyclic
and/or aromatic alcohols. The latter are classed as phe-
nols.

Odorous compounds containing unsaturation, such as phe-
nols, usually are readily oxidizable by ozone. On the
other hand, saturated organic compounds usually are oxi-
dized only slowly by ozone. Since the amount of ozone re-
quired to remove tastes and odors will vary depending
upon the specific offensive organic compounds present, it
is essential to conduct pilot studies with ozonation to
ascertain the most cost-effective relationship of ozone
dosage and contact time to cope with the specific local
problem. As before, destruction of organic compounds which
cause tastes and odors by oxidation does not necessarily
insure total oxidation of those organic compounds to CO~
and water.
 2.7 Algae  Removal

 During  seasonal  periods  of  climate  changes  and when  the
 proper  nutrient  balances are  present  in  the raw waters,
 algae growths  are  promoted. As  these  plants grow,  their
 metabolism produces  by-products which can  cause offen-
 sive tastes and  odors.  In addition, the  presence  of  large

-------
                       - 431 -

amounts of growing algae in the water treatment plant will
clog filters, requiring more frequent backwashing. Ozona-
tion will disrupt the metabolic processes of many types
of algae by oxidizing the essential organic components.
In treatment plants where ozonation already has been in-
stalled for other purposes  (such as disinfection or iron
and manganese oxidation), seasonal blooms of algae in the
raw waters are handled simply by increasing the preozona-
tion dosages until the bloom periods are completed. This
requires installing sufficient ozone generation capacity
initially to cope with this problem.
2.8  Oxidation of cyanides, sulfides and nitrites

Toxic cyanide ions are readily oxidized by ozone to the
much less toxic cyanate ion:

            CN~ + O3 	>  CNO~

At low or high pH, cyanate ion hydrolyzes to produce CO-
and nitrogen:
            CNO
Sulfide ion is easily oxidized to sulfur, then to sulfite
and, finally, to sulfate:

            S~2 + 03 - >  S° — }  S03~2 - >  S04~2
The degree of 'oxidation attained depends upon the amount
of ozone employed and the contact time. Organic sulfides
will oxidize"to sulfones, sulfoxides and sulfonic acids
upon ozonation at slower rates than the sulfide ion it-
self.

-------
                         432 -
Nitrite ion is readily oxidized to nitrate ion by ozone
Organic nitrites, nitroso compounds, hydroxylamines and
the like also will be oxidized, first to the correspon-
ding nitro compounds. These then will decompose upon con-
tinued ozonation, liberating nitrate ions and carbonaceous
compounds. As before, the degree of oxidation will depend
upon the specific compounds present, the amount of ozone
employed and the contacting conditions employed.
2.9  Oxidation of Organics

Although there are literally myriads of organic compounds
present in water supplies  (at the latest count, the U.S.
EPA has identified over 70O individual organic compounds),
not all are oxidized at the same rates upon ozonation. In-
deed, many highly halogenated organic compounds are not
oxidized at all under ozonation conditions normally encoun-
tered in drinking water treatment plants. Rice & Miller  (18)
discuss these aspects in reviewing the nature of organic
oxidation products formed upon reaction with ozone under
aqueous conditions.

During the PTI survey of drinking water plants using ozo-
nation, many plants responding to the questionnaires re-
ported that they are using ozone for "organics removal".
However, in most cases the organics removed are not iden-
tified. Nevertheless, some specific organic materials
known to be readily "removed"  (oxidized) by ozone have
been identified, and these include phenols, detergents
and certain pesticides.

-------
                       - 433 -
2.9. r "Phenols''         '                   '

Phenols, especially the non-chlorinated phenols, generally
are readily oxidized iipon ozonation. It is important to
recognize, however, that destruction of the phenolic com-
ponent requires much less ozone than conversion of all of
the phenolic compound to C0? and water.

Eisenhauer (19) ozonized aqueous solutions of phenol for
3O minutes (until phenol was "destroyed" by the analyti-
cal test used) and isolated catechol, hydroquinone, p-
quinone, cis-muconic acid, oxalic acid and fumaric acids
as organic oxidation products. After 4 moles of ozone had
been consumed per mole of phenol, substantially all of the
phenol originally present had disappeared, but very little
CO2 had formed.

In later work, Eisenhauer (2O) showed that as ozonation of
phenol solutions proceeds, no CO2 is formed until after
1.5 moles of ozone/mole of phenol is consumed. However,
after 33 % of the theoretical CO,, had formed, CCU produc-
tion then ceased. He concluded that if destruction of the
aromatic ring is sufficient to solve the particular local
problem, then 98 % of the phenol can be "destroyed" using
5 moles of ozone per mole of phenol present. However, 67 %
of the original carbon present in the phenol still is pre-
sent in the form of other organic compounds which are oxi-
dation products of phenol.
The oxidation of phenol itself proceeds first through
di- and trihydroxyaromatic compounds and quinones.- Con-
tinued ozonation breaks the aromatic ring, forming ali-
phatic acids, the most stable end product usually being
oxalic acid.

-------
                       - 434  -

Phenols with aliphatic  substituents on the ring are first .
oxidized to benzoic acids, then to hydroxy benzoic acids,
after which the aromatic rings are ruptured. As oxygena-
tion proceeds, the oxidized  intermediate compounds be-
come more readily biodegradable.

Hillis  (21) studied the oxidation of 14 phenols with ozone
over the pH range 4 to  10. Starting with 3O mg/1 concen-
trations of these phenols and ozonizing over 4 to 12 mi-
nutes, phenol concentrations were lowered to O.1O mg/1.
However, the corresponding COD values were lowered by only
5O %. This is further indication that although the speci-
fic phenol is destroyed by ozonation, oxidized organic
products remain in solution.

Nevertheless, the elimination of measurable concentrations
of phenols by ozonation is practised successfully at a
great many drinking water treatment plants  (3).
2.9.2  Detergents

Detergents generally fall into two classes, linear alkyl
sulfonates and linear alkylbenzene sulfonates. There are
some aliphatic quaternary amino compounds which are used
as detergents, however, most household laundry detergents
are of the first two types. The more readily biodegradable
detergents will decompose in sewage treatment plants or in
rivers and streams, given sufficient time. However, many
raw water supplies are contaminated from time to time by
detergents or their partial decomposition products.

As discussed earlier, those compounds containing aro-
matic groupings will be more readily oxidized by ozone,
while the aliphatic materials will be less reactive. In

-------
                       -  435  -

addition,•-••Gilbert (22,23,24) has shown that even aromatic !
compounds containing sulfonic acid groups will be less
readily oxidized by ozonation than the same compounds
without the sulfonic acid groupings.

Gilbert  (23) also has shown that in ozonizing pure aqueous
solutions of compounds over the pH range of 3 to 7, 1 kg
of COD can be removed from solution with 1.2 kg of ozone.
On the other hand, in more polluted wastewaters, 2 to 5
kg of ozone are required to remove 1.kg of COD from so-
lution.

In addition, the excellent work of Hoigne (this meeting)
and of Hoigne & Bader (25,26 and prior references cited
therein) on determination of reaction rates of specific
organic compounds with ozone will allow the practising
water treatment plant engineer to ascertain the ease of
oxidation of specific organic compounds that he may find
in his raw waters.

When ozonation already has been installed in a water treat-
ment plant for some other purpose, the presence of signi-
ficant quantities of detergents in the raw water is easily
recognized by the sudden foaming of the waters during pre-
ozonation or in the first contacting chamber when ozone
is used for disinfection. In such instances merely increa-
sing the ozone dosage usually copes with the problem. As
with seasonal algae blooms, it is important that sufficient
ozone generation capacity be available in the plant.

-------
                          - 436 -

 2.9.3  Pesticides

 Pesticides cannot be classified so simply as to their
 reactivity with ozone. Phosalone and aldrin, for example/
 are readily oxidized to destruction with small amounts of
 ozone.  On the other hand, dieldrin, chlordane, lindane,
 DDT,  PCBs, PCP and endosulfan are only slightly reactive
 with ozone under normal ozonation conditions encountered
 at drinking water treatment plants. Little or no removal
 of these pesticides can be expected with ozone, or with
 other oxidants.

 Malathion and parathion represent two unique cases of
 pesticides which are oxidized to destruction by ozonation,
 but which proceed through intermediates  (their correspon-
 ding oxons) which are more toxic than are the starting
 thions  (27).
    .PSCH-COQC-H
CHoO
  3      £.£.'.
    (malathion)

^WKljL/0
(CH3CH2/
    (parathion)
°3 ,
«f
t MH
0
CH3OJ| 03
ta. ^ *ocf*i i cnnr* 1 1 ^ v^
Jt 5Ln-LUULpHc ^
pij n PU pnnr* u
un-jw onov/uuortni-
(malaoxon)
°3 2 /=r
*fc» Cm nr \ rt n /
(paraoxon)
               -NO,
                     decomposition
                     products
 \
decomposition
products
 Hoffman & Eichelsd6rfer (28) showed that heptachlor is
 oxidized "quantitatively to destruction" with ozone but
 that heptachlorepoxide is stable to ozonation. This raises
 the question as to whether ozonation of heptachlor pro-
 duces heptachlorepoxide, which itself is a very toxic ma-
 terial.

-------
                       -  437  -
          Cl   H  Cl
       heptachlor
                                                  0
heptachlor epoxide
Thus it is incumbent upon a water supply system to iden-
tify the specific pesticides with which it must cope,
then to ascertain the most effective method of removing
them. If ozona'tion is a part of the water treatment pro-
cess, certain pesticides can be removed effectively, but
others will require a different treatment sequence.
2.1O  Suspended Solids Removal (Turbidity)

Turbidity is caused by suspended solids, which are small,
colloidal sized particles having surfaces which are highly
charged. The strength of these surface charges, in fact,
keeps the particles in suspension because of the repul-
sive surface forces coupled with the small particle sizes.
Such colloidal particles normally pass through filters
and are not retained.
In some instances addition of a strong oxidant, such as
ozone, will change the nature.and/or extent of these sur-
face charges, thus allowing the charged particles to ag-
glomerate and be more readily"removed by subsequent fil-
tration. If ferrous iron ions are 'present, ozonation will
oxidize these to the ferric state, as discussed earlier.

-------
                      - 438 -

As these trivalent ions hydrolyze and agglomerate, they
interact with the surfaces of suspended solids, floccu-
lating them and allowing them to be removed from suspen-
sion by filtration.

The use of ozonation alone can accomplish flocculation
without the addition of soluble ions which remain in so-
lution when chemical flocculants are used. A unique exam-
ple of the use of ozone to reduce suspended solids is the
recently commissioned Chino Basin sewage treatment plant,
near Los Angeles, California in the United States. This
secondary treatment plant is required to discharge an
effluent which is very low in suspended solids and in to-
tal dissolved solids, because the water table in the area
is very close to the surface. Simple filtration does not
lower the suspended solids content sufficiently.

Were it not for the stringent TDS requirement,, Chino Basin
could treat its secondary effluent with alum or other
standard chemical flocculating agents. However, such treat-
ment in this case would require an additional step of re-
moving the soluble chemicals after flocculation, coagula-
tion and filtration. The cose of such multiple treatment
is high and the process chosen was the single step of
ozonation. An ozone dose of 1O mg/1 provides the required
coagulation of suspended solids so as to allow their ready
removal by filtration. This 3 mgd plant went on stream in
early 1978 and the process is operating satisfactorily (29)
2.11  Microflocculation

As discussed earlier/ during oxidation of dissolved orga-
nic materials with ozone, oxygen is introduced into many
of the carbonaceous sites in the molecules. Carboxylic

-------
                       - 439 -

     ;, -  . < •:-'.•//•'••..•  - -, .   ', '  ••'.-.-•••  :•-.---  -	;•..'• -.-r.~- -ji';'r '  i1/.
acids, aldehydes, ketones and alcohols are produced,  all
of which are more highly polar than are  the non-ozonized
compounds. These polar compounds are  capable  of  hydrogen
bonding, which effectively increases  their apparent mole-
cular weights. In addition, the simultaneous  presence of
polyvalent metallic cations, such as  iron and aluminum,
with these polar organic groupings leads to flocculation
of the oxidized organics. Therefore the  ozonation  of  clear
waters containing dissolved organic compounds can  lead  to
an increase in turbidity. This process has been  termed
"microflocculation" by water chemists, and has been amply
described in the preceding paper by Dr.  Maier.

It is because of microflocculation that  many  water treat-
ment plants insist upon following ozonation with a fil-
tration step. The plant at Langenau,  Germany, follows
ozonation with a second addition of ferrous iron com-
pounds to increase the rate of microflocculation,  and
produces a very clear water after subsequent  filtration.
2.12  Ozone Pretreatment for Biological Processing

It has been pointed out in many examples given  above  that
the partial oxidation of organic compounds with ozone
renders them biologically degradable at a faster rate
than before oxidation. This fact leads to the conclusion
that if raw waters to be ozonized  (or treated with  any
other oxidant) contain high amounts of dissolved organic
carbon and those carbonaceous compounds are not readily
removed by flocculation and are not readily oxidized  to
CO^ and water by ozone, that ozonation will produce an
aqueous medium high in dissolved oxygen and containing
dissolved organic compounds which  are more readily  assi-
milated by biological organisms. This reasoning has led

-------
                       - 440 -

to the concept of incorporating .biological, treatment^
steps following ozonation. Since ozonized aqueous media
are conducive to the development of aerobic bacteria,
not only can the carbonaceous compounds be expected to
be degraded biologically, but ammonia also can be ex-
pected to be converted to nitrate by biological nitri-
fication processes.

Biological processes promoted by preoxidation  (or by pre-
aeration or preoxygenation in certain instances) can be
incorporated into the water treatment process by addition
of sand or anthracite filters and/or granular activated
carbon filters. It has been known for many years that
slow sand filters and activated carbon units contain
high degrees of biological activity. Following ozonation
with sand or anthracite filtration  (to remove flocculated
materials), then with GAG filtration has been incorporated
into the Rouen-la-Chapelie plant in France and into the
Dohne plant at Miilheim, Germany, specifically to remove
ammonia biologically as well as dissolved organics. This
technique thereby avoids  (Rouen) or eliminates  (Dohne)
the need for breakpoint chlorination, with its attendent
production of halogenated organic compounds which should
be removed later in the treatment. The process has been
termed Biological Activated Carbon by Rice et al.  (3O),
and is being studied at many water treatment plants.

One of the promising secondary benefits of biological
activated carbon is the greatly extended useful life of
the GAG filter media because of the bacterial activity
which is promoted by preozonation (or preaeration or pre-
oxygenation in certain instances). The Rouen plant has
operated satisfactorily since January, 1976 without having
to regenerate its 75 cm deep GAG beds. Later in this mee-
ting, Dr. Jekel will describe the BAG process as employed

-------
                        - 441  -

at the'Donne'plant, 'which'has not had to regenerate its
4 m deep GAC columns since they were installed in Novem-
ber, 1977. Expectations at Dohne are for at least two
years of GAC operational life before regeneration will
be required.
2.13  To Make Water Blue

The unique property of making finished water blue is one
of the primary reasons given for its use at Langenau,
Germany. This plant is located near the Danube River in
southern Germany, and processes mostly groundwater. Du-
ring dry seasons, however, Danube River water also is
treated by a series of physical chemical processes, in-
cluding two ozonation steps. Preozonation is employed
for suspended solids removal and to dispose of off-gases
from the primary ozonation step (for organics oxidation
and microflocculation). The ozonation process is con-
trolled partly by the shade of blue color which is im-
parted to the finished water. When the water is not suf-
ficiently blue, more ozone is added.
3.   Multiple Applications of Ozone

It is important to recognize that even though ozonation
might be installed for a'single purpose, say iron and man-
ganese "oxidation, many other benefits can be derived from
its use. For example, at Rouen, 'Dohne, Wuppertal and
Langenau, preozonation (sometimes with high-speed tur-
bine contactors) aids in the flocculation process. Post-
ozonation at Rouen is for disinfection while the preozo-
nation is for manganese oxidation, organics oxidation and
preparing the following sand and granular activated car-
bon beds for biological conversion of ammonia and removal
of dissolved organics.

-------
                       - 442 -

At the three Diisseldorf plants  (Flehe, Holthausen and.
Am Staad), Duisburg and Wuppertal, ozone's primary func-
tion is iron and manganese oxidation. At the same time
organics are oxidized and disinfection is obtained.

If ozone is applied for, say, color removal, near the
end of the treatment process, a significant amount of
disinfection also will be obtained. The conjunctive use
of contactor off-gases from the primary ozone contacting
chambers can be effective in such multiple ozonation
treatment processes. These off-gases  (which contain as
much as 5 to 1O % of ozone)sometimes  can be .recycled
economically to an early stage treatment step (as at
Rouen, Miilheim and Langenau) . Alternatively, the ozone
in these off-gases either must be destroyed  (thermally,
catalytically, by passing through moist granular acti-
vated carbon) or diluted with air before being discharged
to the atmosphere. If the volumes of  contactor off-gases
are not large, recycling them to an early stage oxidation
step in the total water treatment process can be cost-
effective.
4.   Summary

Early application of ozonation in drinking water treat-
ment was primarily in France for bacterial disinfection.
In the late 1960s, French scientists defined the ozone
contacting time and dosages required for viral inactiva-
tion, and France since has adopted an ozonation treat-
ment standard for this purpose. Viral inactivation can
be achieved if a residual of O.4 mg/1 of dissolved ozone
can be measured at least 4 minutes after the initial
ozone demand of the water has been satisfied.

-------
                       - 443 -

Many other applications for ozone have evolved since
ozonation was installed in Nice, France  (19O6), most
of which are based upon the high oxidizing power of the
gas. In Table 4 are listed the results of the PTI ques-
tionnaire survey of plants known to be using ozone in
the various countries of the world where ozone is being
used to significant extents.

In Figure 1 is shown a "conventional" drinking water treat-
ment process involving coagulation, sedimentation, fil-
tration and disinfection. The known uses for ozonation
are included at the various points in this conventional
process. Note that ozone is used at different locations
in the drinking water treatment process, depending upon
the purpose or purposes for which it is being used.

It is important to recognize that ozonation of dissolved
organic materials will rarely proceed to completion, e.g.
to produce CO2 and water. In most cases two effects will
be noted after ozonation of dissolved, organic materials:

1) Dissolved organics will be converted to more highly
   oxygenated materials which can and do flocculate, re-
   sulting in an increase of turbidity. In these instan-
   ces, ozonation usually is followed by a filtration step.

2) Dissolved organics will be converted to more highly
   oxygenated materials which are more readily biologi-
   cally assimilable. Therefore, the higher the residual
   DOC after ozonation, the greater will be the chances
   for bacterial and slime growths in distribution sys-
   tems, if additional disinfectants (such as chlorine,  '
   chlorine dioxide and chloramine) are not added after
   ozonation. Biological treatment processes after ozo-
   nation have been incorporated successfully into a few
   plants, and the concept is being studied in many more
   plants.

-------
TABLE  4  Uses  of ozonation by  country  responding  to PTI  questionnaires
Ozonation Used For
Country
Great
Britain
Belgium
The
Netherlands
Austria
Switzerland
Germany
France
Canada
USA
No. of
Plants
Using 03
18
9
12
42
150
136
593
20
5
Question-
naires
Returned
6
1
7
5
9
31
64
18
-
Bacterial Viral
Disinfection (a) Inactivation
2 (0) 1
(0) 1
2 (1) 2
5 (4b) 2
6 (1) 5
27 (10) 8
60 (28) 37
13 (3) 9
1 (1)
Fe/Mn T/0 Color
Removal
2 1 6
1 1
Taste 6
Fe-1 Odor 4 6
1
both-4 2
Taste 1
Odor 1
8-both both-11
Fe-1 +
Mn-1 Taste-5 5
5-both both-31 21
fe-2 Taste-5
Mn-1 Odor- 1
both-15 3
taste-3
4-
Organics Turbidity
Oxidation
-
1
3 1
1
3
16 6
23 9
4
(phenols-2)
-
  (a)  No. of plants known to be using 03 as terminal step or sole disinfectant
  (b)  More Austrian plants known to be using 03 as  terminal step (Dobias & Starz, 1977).

-------
      deconplexing organic-Mn
      pretreatment  for
       biological processes
      Fc & Mn oxidation
      f loccu.lntion
      algae removal
      cinsstruction  of
        off-gas ozone
• pretr.eatment  for
  biological processes
 organles oxidation
 color removal
 tastes & odors
- viral inactivation
- bacterial  disinfection
                                                Sand or
                                                Anthracite
                                                  Filtration
influent
 water
                                                                      v
                                                                  ci2,  cio2

                                                                  or C1NH2
                                                                 for residual
                                                  To.
                                                 Distribution
                                                  To
                                                Distribution
 Fig.  1   Typical points of  application  of ozone in
           drinking  water processes

-------
TABLE 5  Major advantages and disadvantages of  ozone
Advantages
Powerful Qxidant
Powerful disinfectant and virucide
over wide temperature and pll range
Mr preparation, ozone generation «nd
contacting systems are easily automated
— but can be controlled manually
Generated en-site as needed -- operating
costs 2-1 U.S. i/1,000 gallons
Disadvantages
Non-Selective Oxidant
Leaves no residual for protection
of network
Gas/liquid contacting is not a
general practice at water treatment
plant
Capital costs for generation and
contacting are relatively high
Safe -- shutting off electricity
ceases 03 generation
Converts many non-biological ly
liuyratltible organlcs to oxidation
products which are biodegradable
Ones'not produce halogenated organics
will rarely oxidize all organics to CO? *
water. Presence of Biodegradable Organics
requires subsequent biological treatment and/or
residual disinfectant, but at lower levels
Does not oxidize highly halogenated organics
Reduces amount of residual disinfectant
required for network
Adds dissolved oxygen to water
Does not react with ammonia below pH=9
Does not increase total dissolved sol Ids
                                                                                        CPl
                                                                                         I

-------
                         447 -•
In addition, it is also clear that because the capital

costs for ozonation installations are high and because
ozone reacts with a" wide variety of materials, ozone
should not be used to perform water treatment tasks that
other techniques can do as well (or better) but at lower
cost. For example, since suspended solids are easily re-
moved by chemical coagulation, this process and filtra-
tion should precede ozonation for'most other purposes.


The> major advantages and disadvantages of ozone in trea-
ting drinking water are listed in Table 5.
(1)  RIDEAL,  E.K,
    Ozone
    Constable  & Co.  Ltd., London, England (1920)

(2)  VOSMAER, A.
    Ozone,  Its Manufacture,  Properties and Uses
    D.  van  Nostrand  Co.,  New York (1916)

(3)  MILLER,  G.W.,  RICE,  R.G., ROBSON, C.M.,  SCULLIN, R.
    WOLF , H. ,  KtJHN ,  W.
    An  Assessment  of Ozone and Chlorine Dioxide Technologies
    for Treatment  of Water Supplies
    EPA Report No. 60O/2-78-147.  U.S. Environmental Protection
    Agency,  Cincinnati,  Ohio (1978)

(4)  BARTUSKA,  J.F.
    Ozonation  at Whiting, Indiana
    J.  AWWA 33 (1941),  11, 2035-2O50

(5)  BARTUSKA,  J.F.
    Ozonation  at Whiting  (Indiana): 26 Years Later
    Public  Works,  August  (1967)

(6)  HARRIS,  W.
    Ozone Disinfection  of the Strasburg,  Pennsylvania, Water
    Supply  System
    Proc. First Internat. Symp. on Ozone for Water and Waste-
    water Treatment. R.G. Rice &  M.E. Browning  (editors)
    Internat.  Ozone  Inst., Cleveland, Ohio (1975), 186-193

-------
                        - 448 -
 (7)  LACY,  W.,  RICE,  R.G.          '      ,  V   ^  '.
     The  Current Status  of Ozone Treatment Technology in the
     United States
     Presented  at Special  Seminar on Water Supply,  Internat.
     Bank for Reconstruction  &  Development, Washington,  D.C.
     Jan  11, 2978-  See also Wasser Berlin 1977,  AMK Berlin
     Germany/Internat. Ozone  Inst.,  Cleveland, Ohio, 1978

 (8)  GUINVARC'H,  P.
     Three  Years  of Ozone  Sterilization  of Water in Paris
     Ozone  Chem.  &  Technol.,  Adv. in Chem. Series,  21,
     Am.  Chem.  Soc.,  Washington, D.C. (1959),  416-429

 (9)  O'DONOVAN,  D.C.
     Treatment  with Ozone
     J. AWWA $2 (1965),  9, 1167-1192


(1O)  HARDEN, C.H.
     The  Boxley Works of the  Maidstone Waterworks. Company
     Trans. Inst. Water  Engrs.  48 (1943), 152

(11)  COIN.-  L.,  HANNOUN,  C., GOMELLA, C.
     Inactivation of  Poliomyelitis Virus by Ozone in the
     Presence of Water
     la Presse  Med. 72  (1964),  37, 2153-2156

(12)  COIN,  L..  GOMELLA,  C., HANNOUN, C.,  TRIMOREAU,  J.C.
     Ozone  Inactivation,  of Poliomyelitis Virus in Water
     la Presse  Med. 75f  38, 1883-1884

(13)  SCHULHOF,  P.
     Private Communication, 1977

(14)  HOIGNE, J.
     Private Communication, 1978

(15)  MASSCHELEIN, W.
     Chlorine Dioxide (Chemistry and Environmental Impact
     of Oxychlorine Compounds)
     Ann  Arbor  Science Publishers, Inc., Ann Arbor,
     Michigan  (1978)

(16)  SCHACK, C.J.,  CHRISTE, K.O.
     Inorg, Chem. JJ5  (1974) ,  378

(17)  K3LLE, W.
     Problem der gemeinsamen  Anwendung verschiedener
     Oxydationsmittel bei  der Wasseraufbereitung
     Vom  Wasser 35  (1968) , 367-381

-------
                        _ 449  -
 (18)  RICE,  E.G.,  MILLER,  G.W.             '-  *      '•'•'<"••  :-'
      Reaction Products of Organic Materials With Ozone
      and With Chlorine Dioxide in Water
      Presented at Symp. on Advanced Ozone Technology,
      Toronto, Ontario, Nov. 1977. Internat; Ozone Inst.
      Cleveland, Ohio (1977)

 (19)  EISENHAUER,  H.R.
      The Ozonization of Phenolic Wastes      > '  '
      J.  Water Poll.  Control Fed. 4O (1968), 11, 1887-1899

 (2O)  EISENHAUER,  H.R.            •
      Dephenolization by Ozonolysis
      Water  Research  5_ (1971), 467-472

 (21)  HILLIS, M.R.
      The Treatment of Phenolic Wastes by Ozone
      Presented at Third Internat. Symp, on Ozone Technol.,
      Paris,  May 1977. Internat.  Ozone Inst., Cleveland,
      Ohio (1977)


(22) GILBERT, E.                    •
    Ozonolysis of Chlorophenols  and Maleic Acid in Aqueous
    Solution"
    Proc. Sec. Internat.  Symp.  on Ozone Technology
    R.G. Rice, P. Pichet  & M.A.-  Vincent (editors), Internat,
    Ozone Inst.,  Cleveland, Ohio (1976), 253-261

(23) GILBERT, E.
    Chemical Reactions Upon Ozonization
    Presented at  Internat. Symp. on Ozone and Water,
    Wasser  Berlin 1977, AMK Berlin/Internat. Ozone Inst.,
    Cleveland, Ohio  (1977)

(24) GILBERT, E.
    Reactions of  Ozone with Organic Compounds in Dilute
    Aqueous  Solution: Identification of Their Oxidation
    Products
    Ozone/Chlorine Dioxide Oxidation Products of Organic
    Materials. R.G.  Rice  & J.A.  Cotruvo (editors), Internat.
    Ozone Inst.,  Cleveland, Ohio (1978), 227-242

(25) HOIGNE,  J., BADER, H.
    Ozonation of  Water: Selectivity and Rate of Oxidation
    of Solutes
    Presented at  Third Internat. Symp.  on Ozone Technol.
    Paris,  May 1977.  Internat. Ozone Inst., Cleveland,
    Ohio (1977)

-------
                        - 450 -
(26)  HOIGNE J., BADER, H.      ..    ,  , .  ,   ,
     Rate Constants for Reactions of Ozone With Organic
     Pollutants and Ammonia in Water
     Presented at Symp. on Advanced Ozone Technology, Toronto,
     Ontario, Canada, Nov. 1977., Internat. Ozone Inst.,
     Cleveland, Ohio  (1977)

(27)  RICHARD, Y., BRENER, L.
     Organic Materials Produced Upon Ozonization of Water
     Ozone/Chlorine Dioxide Oxidation Products of Org. Materials
     Internat. Ozone Inst., Cleveland, Ohio (1978), 169-188

(28)  HOFFMAN, J., EICHELSDORFER, D.
     Zur Ozoneinwirkung auf Pestizide der Chlorkohlenwasserstoff-
     gruppe im Wasser
     Vom Wasser 3J5 (1971), 197-2O6

(29)  NOWAK, T., TATE, C.H., MOUTES, J.G., STONE, B.C.,
     TRUSSELL, R.R.
     Full-Scale Tertiary Wastewater Treatment Plant
     Presented at Ozone Technol. Symp./ Los Angeles, May 1977
     Internat. Ozone Inst., Cleveland, Ohio (1978)


(3O)  RICE,  R.G.,  MILLER,  G.W.,  ROBSON, C.M., KtlHN,  W.
     Biological /Activated Carbon
     Carbon Adsorption,  P. Cheremisinoff & F.  Ellerbusch
     (editors), Ann Arbor Science  Publishers,  Inc.,
     Ann Arbor, Michigan (1978)

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                          - 451 -


THE USE OF OZONE IN THE TREATMENT OF DRINKING WATER

J. Chedal
I should like to comment on the present and future use of
ozone in the waterworks supplying the suburbs of Paris with
drinking water by the treatment of surface water.

For 10 years ozone has been used in the after-treatment after
a physical and chemical clarification.  The main function of
this treatment is to guarantee a viricidal final sterility.
This aim is fulfilled by maintaining a residual ozone level
of 0.4 g/m  in the water for 10 min.  To achieve this,
according to the provisions described by R. Rice, the ozone
is added in consecutive chambers.

When the experiments which I described yesterday are completed
we shall convert this chemical plant into a biological plant
and so make use of ozone in the preliminary treatment of the
raw water before each chemical treatment.  This new treat-
ment process makes it possible to achieve two different aims:

1.   The preliminary break-point treatment with chlorine is
     avoided.   In the new process the ammonia is eliminated
     biologically during the clarification.  The advantage
     of this process is that the formation of haloforms
     during the preliminary treatment is prevented.

2.   During the clarification phase the removal of organic
     substances is increased.  This reduction in their content
     largely affects the precursors.  Consequently, the final
     chlorination for protection of the network gives rise
     to only minimal haloform formation.

-------
                           - 452 -
                   filtration
                       sedimentation
                                            chemical
                                            treat men t
            ~-'^Ts=s*f--r^
             	1     |1	..__..,.:...:_,....	K. _
             ozone
             production
                r  _\  	v — e  -r
The additional removal of organic  substances achieved by
this method of ozonization  is  certainly partly due to the
coagulating action of ozonization.   The various aspects of
this phenomenon have just been presented by Dr. Maier.

To put this new approach into  practice  the measures shown in
the adjoining scheme were taken in the  largest waterworks
supplying drinking water to the Paris suburbs (capacity
900,000 m^/day).  The structures for the preliminary ozon-
ization comprise a container for preliminary chemical treat-
ment, which is currently under construction.
The experiments have shown  that  a  preliminary ozonization
with 1 ppm makes possible an  equally  large  reduction of the
ozone dose in the after-treatment.

-------
                           - 453 -


Accordingly, no additional ozonization equipment need be
installed.  The present ozone generator is adequate for the
after-ozonization plants,.and for .the preliminary ozonization.

The development I have just described shows how careful one
must be in the planning of a w cer-treatment works.  It is
always desirable to make provisions for the incorporation of
additional processes in a treatment plant.

As regards the ozone, it is best if its generation and appli-
cation are kept separate.  To enhance the quality of the
treatment it may be advisable to apply the ozone at several
points in the treatment line, particularly in"the preliminary
ozonization as in the present case.

-------
                           - 454 .-.


SYNERGISTIC EFFECT OF OZONE AND CHLORINE•ON BACTERIA
AND VIRUSES IN SECONDARY WASTEWATER EFFLUENTS

Y. Kott
Synergistic effects are prevalent in nature and are most-
ly studied as factors of growth inhibition; synergism
was also observed when gamma radiation and heat were used
together in order to decrease the number of microorganisms
found in wastewater sludge. The current study, supported
by joint German/Israeli Research Projects BMFT and NCRD
Project No. O13-714, was undertaken with a view to fin-
ding whether or not simultaneous application of chlorine
and ozone to secondary wastewater would involve in a sy-
nergistic effect. Various studies have shown that ozone,
chlorine and other disinfectants reduce microorganism
counts in secondary was-tewater effluents. In the present
study, chlorine at concentrations of 5-30 mg/1, applied
to a continuous flow sample in a column, showed reduction
of coliform at range of bacteria 1O /1OO ml by one order
of magnitude up to five,that is 0-2 bacteria in 1OO ml.
                                                   4
Poliovirus count  (attenuated strain) at range of 1O /5
liter  samples decreased by up to two orders of magnitude
at the most.

When ozone was applied at concentrations of 1O-25 mg/1
to secondary wastewater under the same experimental con-
ditions the count of coliform bacteria decreased from
  5             4
10 /100 ml to 1O /1OO ml which is one order of magnitude,
                                 4
and that of enteroviruses from 1O /5 liters to 3O which
is three orders of magnitude. Different concentrations
of these oxidation chemicals applied for different con-
tact times  showed that chlorine has a much higher kill
efficiency for bacteria than for viruses, while the re-

-------
                          - 455"-'

verse effect was observed with ozone. In addition
Salmonella typhymurium a bacteria representing the pa-
thogens, and Tj bacteriophage representing other viruses
were examined. The results showed that ozone caused an
80 percent decrease for the bacteria and a 95 percent
decrease for Polio I attenuated viruse particles at equi-
valent experimental conditions. When the synergistic
effect of chlorine and ozone was studied, the chemicals
were applied separately, together and in sequence, all
on the same experimental set-up.

Definite synergistic effect was observed with a better
kill effect on bacteria and viruses. The order of se-
quential application made no difference. Further study
on the synergistic effect "'is underway for economic eva-
luation.

-------
                          - 456 - ,; .


EXAMPLE OF UV OZONE CONTROL - ELIMINATION OF RESIDUAL OZONE

J. Valenta
The equipment and apparatus available commercially at the
present time enable continuous monitoring of the ozone con-
centrations both in air-ozone or oxygen-ozone mixtures and in-
ozonized water.

As an example of suitable ozone monitoring I should like to
describe briefly —  from our own practical experience -  the
continuous monitoring of the residual ozone content in the out-
going air of a newly installed ozone eliminator in the lake
waterworks at Lengg.

Here we were able to detect at times certain ozone emissions,
lasting only a short time, particularly during the backwash of
the activated carbon filters.  The situation is illustrated
in Fig. 1.

It was therefore decided to supplement the original thermal
ozone elimination by a catalytic decomposition unit, the
earlier plant additionally assuming the function of preheating
the exhaust gas upstream of the catalyst.'

After the installation of the new elimination plant the per-
formance was monitored with the aid of two continually running
ozone detectors based on the principle of UV measurements. One
instrument, with a 2 cm quartz cell,was placed at the entrance
(position 3), the other, with a 50 cm long cell, was placed
downstream of the catalyst (position 6).  In addition, the
residual ozone concentrations at seven other points along the
air outlet were measured over a short period.  A low-pressure
mercury lamp serves as the light source in these instruments,
providing light with almost 95% of the 254 nm wavelength.

-------
                           -  457 -':
 ELIMINATION OF RESIDUAL OZONE
                  !  Oj-outflow from ACF _
[UJ
1 "0"

ttfV Zun£h. AMSUS
1 	 1
  Fig. 1   Control of the catalytic elimination  of
          residual ozone in the lake waterworks at  Lengg
To obtain the desired  flow-through velocity of the measured
gas mixture, a small ozone-resistant membrane pump and a
rotameter were connected; at-the  second photometer (6).

The flow velocity was  here ^practically a constant., between
0.6 and 0.8 1/min,  in  the course-of several days.
Fig. 2 shows two recorder  charts  with the data evaluation
before the elimination and after  the catalytic decomposition.
The activated carbon  filter .washes-,, during which an earlier
elevated ozone .concentration  in the. outgoing air could be
detected, are also marked.  At the  input the ozone content
was at the maximum 3  mgO/lv  corresponding to about 1500 ppm

-------
                           -  458 -



of ozone,  while after the eliminator 0,01 mg of  ozone/1

 (5 ppm)  was never  exceeded.  The large layer thickness in the

second  instrument  makes it possible to determine ozone concen-

trations down to'0.01 mg O3 per  litre of outgoing air.


This  figure demonstrates the very high performance of the

catalytic elimination of ozone and at the same time the fea-

sibility of using the UV method for this purpose.
 Copy from photo-
 meter charts
                                               ... -   .
                                     *'^^ -^^—Ji*^~= joo%-
                         
-------
                         -  459  -


USE OF CHLORINE DIOXIDE FOR THE TREATMENT OF DRINKING WATER  '
W.J. Masschelein


1.     INTRODUCTION
Though the bactericidal properties of chlorine dioxide have
been known since the beginning of the century, the compound
has only been used in the field of water treatment since the
nineteen-fifties.  It was when chlorine, which was in any
case fairly cheap, had been found not to be entirely satis-
factory when used on its own as a disinfecting agent, that
other agents such as chlorine dioxide began to be used.
Many large Water Boards, such as the Paris one (both city
and suburban), the Dusseldorf Board, and many others adopted
chlorine dioxide.  The results obtained were and still are
very satisfactory, notwithstanding certain reservations
expressed by some users. Owing  to conservatism and ignorance,
frequent attempts were made to use facile pretexts to cast
doubt upon the use of .chlorine dioxide: instability of the
reagent, handling risks, lack of a good method of analysis,
especially for residual quantities, appearance of residual
chlorite for which there was no reliable method of analysis.

It was during this period, and more precisely in 1968, that
the use of chlorine dioxide was adopted by the Compagnie
Intercoinmunale Bruxelloise des Eaux. In its time, this
decision required two years of preliminary research, both
documentary and laboratory investigation  with technical
development.  At present, as we know, the accumulation of
knowledge concerning the formation of organochlorine compounds
has renewed the interest in chlorine dioxide, as indeed in
other possibilities of water treatment.

-------
                         - 460 -

Since th,e time'..allotted-,-for.this, paper does not enable me,tp
develop exhaustively the prospects and the limitations of
chlorine dioxide, I shall refer to earlier monographs  (1,2).
All the same, I shall try to highlight their most important
aspects, and above all the criticisms alluded to above.

2.     PRACTICAL SYNTHESIS OF CHLORINE DIOXIDE
Chlorine dioxide is a gas that must be prepared in situ
where it is to be used.  The synthesis reactions used in
treatment stations are as follows:
and
       2 NaClO2 + C12   = 2 NaCl + 2 C1O2
       5 NaClO2 + 4 HC1 = 4 C1O2 + 5 NaCl + 2
The oxidation of chlorite by persulphate is in most cases
limited to swimming pools:
       2 C1O~ + S2Og  = 2 C1O2 + 2
Synthesis starting from chlorates  (1,2) is hardly ever used.
It is more suitable for production capacities much larger
than those that suffice for water treatment.

2.1.   In the synthesis of chlorine dioxide from chlorite and
chlorine, a minimum concentration of chlorine is required to
enable sufficiently rapid formation of the chlorine dioxide
without any concomitant dismutation reaction that would give
chlorate according to the probable mechanism:
       6 C102 + 3 H2O = 5' HC1O3 + HC1

-------
                        - 461 -•  "«

In fact, "the" acidity of the-water'-due to" the 'chlorine serves^
mainly to neutralize the alkalinity introduced by the
stabilized chlorite.  At initial chlorine concentrations of
the order of 0.4 to 0.5 kg.m"  one must work with approxi-
mately equal weights of chlorine and sodium chlorite, namely
an amount of chlorine equal to 200 to 250% of the stoichio-
metric amount.  With 1 to 1.5 kg of chlorine per m  in the
initial solution the reaction attains a 90% yield, the final
pH of the soluiton being between 4 and 5.  At higher
chlorine concentrations, up to 3 g.£  , a stoichiometric
mixture with sodium chlorite gives a yield greater than 95%,
at a final pH lying between 5 and 6.  This solution also
contains up to 6 g of C1O2 per litre.

If one operates at these concentrations, the equilibrium
vapour pressure of the dioxide gives rise to a concentration,
in the gaseous phase, of 6 vol-% at 15°C and 10 vol-% at
30°C.  After the reaction phase it is thus necessary to
dilute the solution  if the reagent is to be preserved for
a certain time before being injected into the water to be
treated.  This operation is advantageous mainly in that it
permits metering in proportion to the flow rate of the water
being treated.  The dilution will keep the pH between 5 and
7.5, so that both acidic and alkaline dismutations will be
avoided.  The final concentration attained is between 0.5
and 1 g of C1O2 per litre.

2.2.   For the surplus  (see Fig. 1) the following safety
measures should be taken:

       automatic cut-off of chlorite in the event of insuffi-
       cient chlorine;
-      cut-off of gaseous chlorine in the event of
       insufficient water

-------
                            -  462 -

         provision of  a dilution zone within the  reactor;
         ventilation of any  storage tanks,  which  will also
         avoid any build-up  of  pressure  inside them;
         safety vents  on the pipelines leading the gases
         towards the ClOp elimination plant: graphite plates
         for  the CIO™,  or sodium hydroxide  exchanger, depending
         on the capacity of  the installation;
         all  the joints and'  connections  of  the reactor and
         the  plant must be made using flame-proof materials
         and  installation methods;
         free ventilation, or preferably an extractor fan, for
         the  chlorite  storage hopper.
 NEUTRALISATION
 OU CHIORE
DOSEUR  COL0NNE
        DE
             CHLORE  REACTION
                                 RESERVOIRS 06 STOCKAGE
                                         STATION 0£ FILTRATION
                                          (mlcrotamisogt)
                |MS $an0
              gkslaus
     * **«
 -6-
  9
  O  *»»«*«*•• » i*****1*     ^u  P,«4 «»
 Sc'qixncts automatiquta - production cJu txaxydc de chEort
L«K Man *?u$t.ira 11
L "6f*5.E_2_J     STOCKAGE DU CHLORITE DE SODIUM
P""*"
Ui- *«.»»* <
           i-iMiWI* »8'fc, DC ,* »«OOiX:!iO"
Fig.  1   Plant for the  preparation of ClO^ from
          and  Cl.  Example: the  Lienne  station-

-------
                         - 463 -

                        :.  . , .   •• i  > ••»..•••  . '•' •  v • : '•' ~' '
Reactors similar to,the one illustrated here  and capable of
producing up to 7.5 kg/h of C1O2 have been operating for
several years at the Tailfer station.

2.3.   The direct reaction involving stoichiometrie propor-
tions of chlorite and hydrochloric acid,and producing
                                                   M* "I
chlorine dioxide at concentrations of 0.5 to  2 g.£  ,  only
proceeds with a yield of 60 to 80%.  Various  secondary
reactions result in the formation of chlorine:

       5 C1O~ + 5 H+ = 3 C1O~ + C12 + 3 H+ +  E-^O
       4 C10~ + 4 H+ - 2 C12 + 3 02 + 2 H2O

 Moreover, the chlorous acid appearing as an intermediate in
 the reaction can undergo dismutation according to:

        4 HC1O2  = 2  C1O2  + HC103 + HC1 + H2O

 Yields of up to 100%  for C1O2 formation,  i.e. the production
 of four moles of dioxide from five moles of chlorite,  may be
 attained provided that the reagents NaClO,-, and HC1 are mixed
 in equal weights,  in  the form of solutions with concentrations
 of 7.5 wt-% of NaCl02 and 9  wt-% of HCl.   These conditions
 imply a final pH of less than 0.5.

 This technique means  that the commercial chlorite, at 24-
 25 wt-% (300 kg.m~  )  must be diluted,  an operation best per-
 formed with softened  or  demineralized water so as to  avoid
 the precipitation of  calcium carbonate and calcium silicate
 (with magnesium oxide)  in the  solution used.  To avoid these
 difficulties, we at C.I.B.E.  have developed a high-concen-
 tration process based upon the direct use of commercial
 300 kg.m   sodium chlorite and acid diluted 1+6.  Under
. these conditions the  reaction is complete within about 10 min
 and the d
 25-30 g.fc"
and the dioxide is produced at a concentration of some
         -1

-------
                         - 464 -
Pig. 2   General layout of-this type of plant
The yield depends on the design of the zone in which_ the
reagents are mixed within the reactor.  A contact enclosure
in the shape of an inverted cone with tangential inlets for
the reagents gives excellent results.  The reactor can be
reliably brought into operation by maintaining a reduced
pressure with an ejector that draws off both dissolved and
gaseous dioxide.  For safe operation it is essential to
provide automatic shut-down in the event of an insufficiency
of the dilution water, by means of the ejector or by dis- •
continuing the reduced pressure.  The other precautions,
such as ventilating the pipelines, fire-proofing safeguards
for the chlorite storage, etc. are similar to those for the
chlorite + chlorine reactors.

-------
                         -  465  -

2.4.   Preparation in the laboratory
If 1.5 to 2 moles of acetic anhydride per NaCIO- are made to
react with an aqueous solution of sodium chlorite, a pure
solution of chlorine dioxide is obtained with a yield of 50%
(3).  The remainder of the chlorite is transformed into
chlorate, together with traces of chlorides, while1the
acetic anhydride is converted into the acid and acetate.

Such as it is, this solution is satisfactory for most appli-
cations in the laboratory or on a small scale, in which the
impurities mentioned above do not interfere.  If the dioxide
needs to be purified, this can be done by scrubbing with air
and redissolution.  This operation is performed in the dark
to prevent any dismutation (4).

3.     METHODS OF ANALYSIS

3.1.   lodometry is still the best method of determination,
both for the chlorine dioxide and the chlorite, provir^d
that other oxidizing agents that can oxidize iodide to
iodine are absent.

At pH 7 the reaction is:

       2 C102 + 2 I~ = I  + 2 C10~

At pH 2 the chlorite reacts according to:

       C10~ + 4 I~ + 4 H+ = 2 I2 + 2 H20 + Cl~

Under the practical treatment conditions the chlorine is the
major source of interference.  Because of this, the iodo-
metric method is mainly suitable for calibration.

3.2.   The simultaneous determination of several oxidants,
such as chlorine dioxide, chlorite, and chlorine, often

-------
                         - 466 -

entails an alkaline dismu'tation phase of  the dioxide  into
chlorite and chlorate:

       2 C1O2 + 2 NaOH =  NaClO2 + NaClC>3  + H2
-------
                        -  467  -

water.-  This method involves (6)  decolouration of the reagent
in an ammoniacal medium buffered to pH 8.1 to 8.4.  This is
measured at a wavelength of 550 nm  (a compromise for the
hypsochromic shift of the absorption band).

With the familiar spectrophotometrie techniques the sensi-
tivity and the precision are 0.04 g ClO9.m~ .  A value of
             -1
0.02 g ClO-.m   may be attained by microcell techniques and
by thermostatting the test solutions.  The method'can also
be used for the determination of ozone (7).  Reference should
be made to that publication for certain operating precautions
additional to those described in the original work.  In
practice, interference from ozone need not be feared since,
like the chlorite in solution, the chlorine dioxide is
oxidized to chlorate under the action of ozone.

The use of the ACVK method has been proposed for the determin-
ation of chlorine dioxide in applications relating to the
treatment of waste waters  (8).  Since the method is colori-
metric, there is a possibility of interferences in this case,
but investigation has established a rigorous parallelism
between the results of the colorimetric method and the
results obtained by analysis of the nuclear paramagnetism.
This latter absolute technique is difficult to apply in
practice, but it has been used to confirm the specificity
and the reliability of the ACVK method.

3.5.  'The formation of residual chlorite by reduction of
chlorine dioxide is an awkward feature in the application of
chlorine dioxide to the treatment of drinking water.  It
makes it necessary to control the residual chlorite in the
water, especially since this compound can be toxic when its
concentration is too high.

The only specific and sufficiently sensitive method that we
can suggest is based on a technique involving the differen-

-------
                        .- 468 -

tial plotting of polarograms obtained by means of pulsed
currents.

The electrode reaction is based on the following over-all
equation (9) :
       HC1O2 + 4 e + 3 H
      Cl  +  2  H20
We recommend the following specimen pre-treatment  (10) :
adjust the pH to 4.4-4.5 with a buffer, so as to produce  the
final concentrations CH3COONa 0.04 M> CH_COOH 0.06 M,  and
    )^ SO^ 0.01 M-.  If present, chlorine and chlorine
dioxide are removed by bubbling nitrogen through the
measuring cell.   (If the presence of heavy metal ions  in
concentrations that may cause disturbances is  suspected,  the
water should be passed through a strong cation-exchange
resin) .
The polarograms in the pulsed mode are recorded and, by
difference, a linear relationship is obtained between  the
differential diffusion current and the concentration of
dissolved chlorite.  The sensitivity is 0.05 g Ciol-m"  (10).
   /lA.
           1,03
                                0.11
                                           Pig. 3

                                           Chlorite polarogram
                                           (graphical construktion
                                           by difference, in the
                                           pulsed mode)
           0.5
1.0
1.5

-------
                         - 469 -
3.6.   The practical use of chlorine dioxide in the :treatment
of drinking water often entails continuous monitoring of the
residual oxidants.  In this connection we have developed an
amperometric analyser for the iodine obtained when iodides
are brought into contact with oxidizing agents.

The analyser is based on the galvanic couple Ag/Au which
gives a zero residual diffusion current.  A mixture containing
       3                                     3
0.090 ,m  of acetic acid and 800 g of KI per m  is added to
the water being analysed, so ,as to obtain a final pH of 5.
Stabilization of the latter is based upon the buffering
effect due to the bicarbonate present in the water.

The direct output current obtained using curved electrodes
                       2
with an area of 3000 mm , separated by a distance of' about
50 mm, and with a 1000 fi resistance connected in series, is
          —1  —3           -   •
12.5 mA.Eq  .m  .  The chlorine, the chloramines, and the
chlorine dioxide are shown up in accordance with their
capacity to oxidize iodides .at pH 5.  Chlorites do not inter-
fere.  The sensitivity its of the. order of 0.01 g ClO^.m"
(11)•                    .   .
4.     RISKS INHERENT IN THE USE OF C1C>2

4.1.   In the gaseous states'chlorine dioxide is spontaneously
explosive at concentrations higher than 10 vol-%  (12).  The
reaction is induced by any source of ignition.  At  20°C,
this concentration in the gaseous phase corresponds  to  a
concentration of about 8' g.a~  -of C1O~ in solution.   The
acidification process thus generates a dangerous  atmosphere.
The maximum allowable concentration in working premises is
0.1 ppm by volume for an 8-h work shift per day,  and 0.3 ppm
for brief occupations.  Chlorine dioxide is perceptible in
the air at concentrations of 1.4 to 1.7%.  At 4.5%  it
irritates the respiratory mucosae and .produces severe headaches.

-------
                         - 470 -

^ \ fr - . ' 1 - ™ ' t '' f.  * - ~ | " •' " r '  . _ •  1 H '   .       '  ' .    . t    . ,    , \L ^ ;
There is no cumulative effect  in the event of repeated
exposure (13).

4.2.   In drinking water the maximum residual consent that
does not affect the taste or produce a disagreeable odour is
of the order 0.4 to 0.5 g "C1O2 per m .  This concentration
is also lower than the maximum allowable concentration from
the point of view of toxicity, since tests on rats have not
shown any serological toxicity at doses as high as 5 g.m
 (14).  In Belgium the maximum concentration of chlorine
dioxide tolerated in water distributed through the public
mains is 0.25 g.m

4.3.   At least some of the dioxide used is likely to be
transformed into chlorite by reaction with organic compounds,
The chlorite is toxic and can cause methaemoglobinaemia.
                                                          _ -I
However, the LD^ of sodium chlorite for rats is 140 mg.kg
On the extreme assumption that all of the dioxide could be
transformed into chlorite, this value of LD50 corresponds to
105 mg C102.kg  .  Consequently, there seems to be no
objection to the use of chlorine dioxide, in the usual
amounts, on these grounds.

5.      ADVANTAGES OF CHLORINE DIOXIDE TREATMENT

5.1.   It has been found that, the cost of post-disinfection
with chlorine dioxide, compared to that of chlorine, is
1.2 to 1.7 times as high  (15,16).  However, the relative
cost of treating raw water can change appreciably as a
function of local parameters.  Thus, ratios of 1/1 to 1/4
have been found (17).   The raw water is treated at the
Tailfer station of the Compagnie Intercommunale Bruxelloise
des Eaux both with chlorine and the dioxide.  The average
                                       -3              -3
amounts have been equal to 1.3 g ClO2.m   and 6 g Cl2.m  ,
corresponding to a respective cost of 1 to 2.4 for the
chlorine compared to the dioxide used.

-------
                       -  471  -

5.2.   The use of chlorine dioxide .constitutes an alternative
to prechlorination. One of its  advantages  is  the  smaller
tendency to form organochlorine derivatives, bearing in
mind the reactivity of organic derivatives with this
oxidant  (2).  Moreover, chlorine dioxide forms fewf if any,
simple chlorinated hydrocarbons of the chloroform type  (18).
Another, often decisive, advantage consists in the fact  that
chlorine dioxide does not react with dissolved ammonia.
Thus, it is not essential to use amounts greater than the
critical point to obtain satisfactory disinfection.  This
point is also important for the treatment of swimming pools.

Like ozone, chlorine dioxide leads to micelle formation  in
the coagulation-flocculation pre-treatment.  Because of  this,
the turbidity of swimming pool waters treated with chlorine
dioxide is lower than that obtained by a comparable treatment
with chlorine (19).  Moreover, iron and manganese, which may
be present in the reduced or complexed states, particularly
in association with fulvic or humic acids, are oxidized  and
eliminated by subsequent precipitation.

One of the most important aspects of using chlorine dioxide
for the pretreatment of river water is the competition between
the oxidation reactions that produce chlorite and the dis-
mutation into chlorate and chlorites.  The respective
reactions are:
       C1O2 + 1 e   = C1O2
     6 C102 + 3 H20 = 5 HC103 + HCl

According to this scheme the C1O~/C1O_ mass concentration
ratio of 1 corresponds to a molar concentration ratio of 1.24,
Thus, if the C1O2/C1O~ mass ratio is observed to be 1, about
7.4 moles of initial dioxide will have reacted for each mole
lost by dismutation.

-------
                        -  472 -

This dismutation of chlorite is' slow"in the absence of light.'"
The following reaption is of minor importance except in the
event of intense insolation:

       3 C1Q~ = 2 C103 + cl~

 Experience  has  given  the  following"recommendations  in  relation
 to the practical  use  of chlorine  dioxide  for the treatment of
 raw water:

 -      A reaction time of 30 to 40  min is sufficient.
 -      The  residual C109  concentration after this time
                                        — 3
        should not normally sec eed  0.2 g.m
 -      The  chlorite/chlorate mass ratio after this  action
        should be  between  1 and 2, corresponding  to  an
        effective  degree of reaction of 83 to 91%.  For a
        decantation tank open to the air,  if the  residual
                                              —1
        concentration  of C1O~ exceeds 0.2  mg.£  , the  loss
        of CIO,,  by dismutation into  chlorides and chlorates
        increases.

 Moreover, the decomposition of chlorine dioxide  by  active
 carbon,  as  used in coagulation-flocculation,  is  appreciably
 less rapid  than that  of chlorine  (20). Thus, it becomes
 possible to maintain  a bactericidal activity in  sludges con-
 taining flocculated carbon.

 5.3.   During post-chlorination,  chlorine dioxide can  main-
 tain itself in  clean  water for up to 48 h.  Thus, its
 bactericidal efficacy is  guaranteed for a longer time  than
 that of chlorine.  The same conclusion is relevant  to  the
 persistence of  a  disinfecting action in swimming pools (21).
 The disinfecting  action of chlorine dioxide is recognized to
 be at. least equal to  that of chlorine, and superior in the
 case of waters  with a pH  higher than neutral. In this

-------
                        - 473 -

connection we refer to our previous analysis to  support  this
    * .v '.•,.'    - .           L   <  '•  -, •   •'"  'r.  ' '  * *f,  ' ' '  . -~ k / r , *
conclusion  (2).  It seems, _ in fact, that  the diffusion of
chlorine dioxide depends very little on pH compared  to that
of  chlorine in its various forms in equilibrium.   I  shall
not present here, an exhaustive  review of  the bactericidal
and disinfectant properties of  chlorine dioxide, since this
lies outside the scope of .the present paper, and merely  refer
to  previous reviews of the subject.

5.4.   After the reaction a significant proportion of the
chlorine dioxide is reduced-"to  chlorite.  It is  thus
interesting to examine the bactericidal or bacteriostatic
properties of the chlorite ion, in relation to post-
,disinfection.

The disinfectant power of the chlorite ion is minor.  Thus,
the philosophy of post-disinfection with  chlorine  dioxide
rests on the action of chlorine dioxide itself, which is a
strong bactericide, followed by that of the chlorite, which
is weakly bacteriostatic"and weakly bactericidal.  The
chlorite, in its own right, is  not used directly in dis-
infection.  However, we have tried to determine  its bacteri-
cidal and bacteriostatic effects.

5.5.   The bactericidal effect  upon enterobacteria is shown
in  Figs. 4 and 5.

In  physiological water, type C  coli were  removed,  during the
experiments, in accordance with Chick's kinetic  law:

       ,    N     ,          '•••••
       log N^ = -k!0 t        .  ,.

In  this equation         .  . . , •

       k!0 = 2^3" = -°-075 day"1.

-------
                         - 474 -

In other words, under "the conditions of  the  experiment ,  the
time required to produce  50%  mortality is of the  order of
4 days in the absence of  any  bacteriocide.

In the presence of sodium chlorite  a clear increase  in the
mortality appears at concentrations above 0,2 g ClO-.m
This degree of mortality  no longer  corresponds to Chick's
law.  To interpret it, we subtracted the mortality in the
absence of chlorite from  that observed in its presence.
Moreover, we based ourselves  on the theory of multiple site
inactivation, in which it is  considered  that a certain number
of sites must be reached  at least once if the organism is
to be completely deactivated.   Since the  kinetics  are first-
order for each site considered, the probability of deactiv-
ation of  m  sites is as  follows:

                       N   - N
      _  ,.    -kt,rn  »>  o  .
      Pm=il - e   J   —   j-j      (fraction of organisms killed)
                          o

ThUS     f- -  1 - Pm  -  1 - (1 - a"Kt)m
          o

Binomial expansion gives:

                                  -Kt  m Cm  - 1)
        Pm  =  f1 - 8j  .,  1 _ m a
            -  1 - m e-kt
Thus, the fraction  of surviving organisms  is:
                        o

and           loS
-------
                           -  475 -
   -1
   .2
                                Coli C
                     = ,= -0.35 PAR JOUR
                      ZJ     PEP DAY
                             PRO TAG
0.2 OPK CL02

PAR RAPPORT A
COMPARED TO
BEZOGEN AUF
O PPM CLOj
                                            T, JOURS/DAYS/TASE
                                                                 -TO
Fig.  4     Action of chlorite on  coli  C
 ,0.25.
 -0.25.
 -O.b .
  -0.75-
       l03= 0.25;  = 1 6
                                            ENTEROCOOUBS
                                            ENTEROCOCC!
                                            ENTEROKOKKEU
                                                         JOURS/DAYS/TACrE
 Fig.  5    Action  of  chlorite  on  enterococci

-------
                          -  476
 As far as enterococci are  concerned (a wild ,s.train Isolated
 from the water) , a similar argument gives in ~ 1.8, or about
 2.  These may have been diplococci.
 5.6.    Among the ubiquitous microorganisms, Pseudomonas
 strains deserve special attention since they show a  capacity
 for rapid regeneration in treated waters.

 Of the pure strains, Pseudomonas cruciviae has no capacity
 for surviving longer than a few hours in water.  The wild
 strains that we were able to obtain in cultures  seemed
 generally sensitive to chlorite.  In contrast, Pseudomonas
 putida is more resistant.  According to an analysis  similar
 to the preceding one, the mean number of "sites" was about 6,
 while  the concentration factor was of the order  of 0.3.
                           PUTIDA - CMerltt
                       I OB 
-------
                            477 -
5.7,    Certain cultures were 'found to constitute exceptions
to  the rule.  Among these,  we isolated, on a  King F medium,
a Rodotorula, which is a  ubiquitous non-pathogenic species
that  can be found among the organisms colonizing the human
body.   Whereas in water untreated with chlorite the bacterial
count decreases progressively as a function of time, it seems
that  in the presence of chlorite, after a first phase of
decline, the microorganism  can adapt and maintain an increased
level of vitality (Fig. 7}.  This observation is reported at
face  value,, but it still  requires detailed checking before
any conclusions can be drawn.
     leg N
       s -
                    tEVURE TYPE RODOTQRULE (CHLORITE)
                    ROBDTORUIA YEAST (CHLORITE)
                    HEFEBAKTERIUM VOH TYC RODOTORULA
                                    r
0.2
                        \.
                              X /
                               X
                                 ^
                                         0.0
                                                     HEURES
                                                     HOURS
                                                     STUKDEN
Fig. 7   Action of chlorite on Rodotorula

-------
                        - 478 -
                                 ">- ',

6.     CONCLUSIONS
Though chlorine dioxide has already been in use for several
decades for the .treatment of drinking water, it often
remains an ill-understood reagent.  The problems posed by
the formation of organochlorine derivatives by chlorination
have resulted in a renewed interest in the use of C1O2•
This reagent has great potential: chlorination reduced or
eliminated, ability to oxidize organic compounds, higher
bactericidal activity in alkaline media compared with
chlorine (this activity is maintained in the presence of
dissolved ammonia without the need to overchlorinate beyond
the critical point), etc.

In the present paper I have reported specific data concerning
the use of chlorine dioxide, especially in relation to areas
of difficulty:

-      Dangers of preparation and handling.
-      Toxicity and effects of residual concentrations.
-      Analytical methods for the specific determination of
       chlorine dioxide and residual chlorite.
-      Bactericidal and bacteriostatic effects of the
       dioxide used for post-treatment.

It seems that the objections often formulated against chlorine
dioxide do not constitute valid sanitary'grounds for opposing
its use.

-------
                         - 479 -
 (1)   MASSCHELEIN, W.
      Les oxydes de chlore et le chlorite de sodium
      Ed. Dunod (1969)

 (2)   MASSCHELEIN, W.J.
      Chlorine Dioxide (Chemistry and' Environmental Impact
     •of Oxychlorine Compounds)
      Ed. Ann Arbor Science (in press)

 (3)   MASSCHELEIN, W.
      Ind. Eng. Chem. Prod. Res. Develop. £  (1967), 137

 (4)   APHA-AWWA
      Standard Methods (1975)    '

 (5)   HODGEN, H.W., INGOLS, R.S.
      Analytical Chemistry 26 (1954), 1224

 (6)   MASSCHELEIN, W.                      ,
      Analytical Chemistry 38 (1966), 1839

 (7)   MASSCHELEIN, W.J., FRANSOLET, G.
      J. AWWA 69 (1977),  461

 (8)   KNECHTEL, J.R., JANZEN,  E.G., DAVIS, E.R.
      Analytical Chemistry 5_0 (1978), 2O2

 (9)   HARTLEY, A.M., ADAMS, A.C.
      J. Electroanal. Chem., J5  (1963), 46O

(1O)   MASSCHELEIN, W.J.,  DENIS,  M. , LEDENT, R.
      (in preparation)

(11)   MASSCHELEIN, W.J, FRANSOLET, G.
      (in preparation)

(12)   U.S. National Board of Fire Underwriters,
      Report No. 7 (1949)

(13)   HALLER, J.F., NORTHGRAVES, W.W.
      Tappi 38 (1955),  38

(14)   FRIDLYAND, S.A.,  KAGAN,  G.Z.
      Gig. Sanit. 36 (1971), 18

(15)   BOGNOLESI, U.
      Igiene Mod. £6 (1953), 197

-------
                         - 480 -,
(16)   GOMELLA,  C.
      Techn.  Sci.  Mimic. 56 (1961), 1.71

(17)   DOROLING, L.T.
      Water Treatm. Exam. 23- (1974), 19O

(18)   VILAGINES,. R., MONTIEL,  A., DERREUMAUX A., LAMBERT, M,
      96th Annual  AWWA Conference Anaheim (1977)

(19)   BANDI,  E.E.
      Mitt. Geb. Lebensmittelunters. Hyg. 58 (1967), 176

(2O)   MASSCHELEIN, W., GOOSSENS, R. , MINON, M.
      Tijdschrlft  BECEWA (in press)

(21)   ASTON,  R.N., VINCENT, G.P.
      Proc. 9th Ann, Course Water Plant Operators
      10 (1947) , 54

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                        _  481.'.V-  -


PRACTICAL EXPERIENCE WITH CHLORINE DIOXIDE AND
FORMATION OF BY-PRODUCTS                    ,      .

T. Hedberg, B. Josefssort, C. Roos, B. Lindgren and
T, Nemeth
1 . Introduction

The use of chlorine dioxide in water treatment plants
has increased during the past years. In an EPA-report
(1 )• its extensive use in such countries as West Germany,
Switzerland and France are mentioned. Its use in Sweden
is also becoming increasingly common. Chlorine dioxide
was introduced in 1968'at the water treatment plants
in Gothenburg (70 million m per year). In Sweden today
about 15% of the supplied water is treated with chlorine
dioxide. The reason for its use is, as in most countries,
to improve taste and odour, and at the same time in-
crease the disinfection capacity in the distribution  -
system.
Although chlorine dioxide has been used during the last
ten years in Sweden, research within this field has only
recently started. The general aim is to investigate the
oxidation efficiency, by-products and usefulness of
different oxidants for the typical surface waters in
Sweden. The Swedish waters contain in contrast to most
continental waters high concentrations of humic sub-
stances and thus they are interesting from a treatment
point of view. In addition the waters are - especially
in the western and northern parts of Sweden - very soft
witn a low content of salts and are also rather acidic
as a result of long distance transport of air pollution.
Since soft waters with"a low buffer capacity are very
aggressive to pipes.it is common to adjust the pH value
to 8-8.5 before distribution. ,

-------
In addition to the use of chlorine dioxide in water treat-
ment it is also employed in the industry for pulp bleach-
ing. This is in some aspects a related process since in
both cases lignin-type compounds are affected. Thus an
account of this process is also presented.

2. Formation and behaviour of volatile halogenated orga-
   nic compounds by chlorine dioxide - chlorine treat-
   ment of water in Gothenburg water treatment plant

There has been much concern over the formation of halo-
forms during.the chlorination of water.
In search for safer oxidation techniques attention has
been drawn to chlorine dioxide. However, the use of
chlorine dioxide may also create undesirable by-products.
Chlorine dioxide is a strong oxidant and exhibits a
wide spectrum of reactivities towards organic compounds
(2). However, to date no chlorinated by-products from
the treatment of drinking water with chlorine dioxide
have been reported.
Studies of chlorine dioxide bleaching of pulp (section 3)
and laboratory experiments  on organic model substances
provide evidence for production of considerable amounts
of organic by-products. On the other hand the concen-
tration of reaction products arising from the use of
chlorine dioxide in drinking water seems to be compara-
tively low. Since no volatile organic compounds have
been identified as by-products, one must assume that
these by-products are dominantly non-volatile. This de-
mands new techniques for analyzing non-volatile com-
pounds in low concentrations.
Presented below are results from analyses of volatile
halogenated compounds at different stages in a water
treatment plant in Gothenburg, Sweden, that utilizes

-------
                          - 483;-,-


combined chlorine-chlorine dioxide treatment. The
occurrence of these by-products in the distribution
net has also been investigated.
The analyses of volatile organohalogens in the drink-
ing water were carried out with two different methods
during a two-week period a: closed-loop stripping
                               2
after Grob  (3) followed by  (GC) -MS and b: pentane
extraction followed by glass capillary column separation
and electron capture detection,  (GC)^-ECD  (4). The  re-
sults are presented in Table 2-1. This work has been
reported elsewhere  (5) in detail.
TABLE 2-1  Concentration of organic compounds  in drink-
           ing water. (The compounds marked with an  aste-
           risk were enriched by pentane extraction.)
Halogenated hydrocarbons
Dichloromethanex
Trichloromethanex
1,1, 1-trichloroethanex
Tetrachloromethanex
V
Trichloroethene . ' •
Bromodichloromethanex
X.
Dibromochloromethane ,
Tetrachloroethenex
Dichloroiodomethanex
Tribromomethane
Trichloroaniline
Bromonaphthalene
Drinking water, ng/1
< 6O
95OO
60
17
15
22OO
6OO
8
7
16
1
26
Twelve halogenated organic compounds were  found of
which some were originally -present in  the raw water,
e. g. trichloroaniline..

-------
                         — 484^--
Fig. 2-1 shows the flow scheme of the plant and the
concentrations of halogenated compounds. -Notable are
the markedly increased'concentrations of carbon tetra-"
chloride after.the different chlorination points. How
ever, this increase' is not observed in  the prechlorina-
tion step of raw water with a high concentration of ,
suspended solids. There seems to be an  influence from
sedimentation of particulate matter. The increased
concentration of carbon tetra^chloride probably origi-
nates from the, added chiprine gas.which normally con-
tains 25-100 mg carbon tetrachloride/kg. The reaction
of hypochlorous acid with.chloroform by an electrophilic
attack to yield carbon tetrachloride seems to be insigni-
ficant because'of the -slow reaction rate (6).
Seasonal variations in water quality, e.g. fulvic acid
concentration will affect halofprm formation. Samples
taken during the winter (Fig. 2:-1) show lower contents
of haloforms compared with,samples taken during the
spring  (Fig. 2-2).  '    ;     '  ',  ••
Concentrations of haloforms in the distribution net
over a distance of 30 Km" in the" Gothenburg region
(population 600 000) are illustrated in Fig. 2-2.
Preliminary evaluation of the. results.shows an increase
of the haloforms with distance .from the plants. This
might be explained by the generally•increasing pH
value -with distance. The 'change^ in pH .value is of course
dependent on the pipe ma-tier ials: used (concrete-and
cement-lined pipes) .  - -  -, - - -   .

Formation of haloforms- from chlorine, chlorine dioxide
and different combinations of these "•'•

Haloforms are produced; during chlorination of drinking
water. It has been demonstrated that they are formed
from degradation product's of humic substances  (7) which
are present in all natural waters.

-------
                                                     WATER WORKS
1
Raw

CH CI3
CHBrCI2
CHBr2Cl
CHBr3
CCI4
CHCICCI2
C2CI4
I
i Pre chlorination i Pre chlorination
i 0.7 g/m3 0.5 g/m3


0.14
n.d.
h.d.
n:d.
0.006
0.036
0.012

1
r
1
Lake
reservoir
i
3.5
0.5
0.013

n
.d

0.004
0.027
0.090
1.1
0.1
0.003
n.d.
0.004
0.027
0.090

1
8



2
0
n
r
Alkali-
nisation I
1
.9
.1

23
.d

0.116 .
0.018
0.060
8.3
FJoccul,
sediment.


0.6
0.04
n.d.
0.090
0.018
0.090

1
i
Activated
carbon
filter

4.5
0.6
0.06
n;d.
0.050
Q018
0.012
Post chlorination I
chlorine + chlorine dioxide I
0.3 g/m3 + 0.3 g/m3 I
r ,
Alkalin. Or
h * ^
\

silica

i
4.5
0.7
0.06

n
.d.
aoa4
Q027
0,006
7.7
2.0
QSO
0.009
0.108
0.108
0.003
nking I
water
I
J
I
03
Ul
I
Fig.2-1  Halogenated compounds in the LackarebSck Treatment Plant

-------
                                486' -
              25
              20
            g 15
              10
                                          i
                                   _L
                            10      15     20

                            DISTANCE , KM
25     30
                             GOTHENBURG
                                    ALILYCKAN WATER TREATMENT PLANT
                                        LACKARBACK WATER
                                        TREATMENT PLANT
                                       O 2  4  6810km
Fig.  2-2   Concentration  of trlchloromethane  in the
            distribution net    :

-------
                         -  487  -
In a series of experiments on a.; laboratory scale,, the
formation of haloforms has been studied. River water
(pH 6.88), which is the raw water for the water-works
in Gothenburg, was treated with chlorine, chlorine dioxide
and different combinations of these. Analyses of halo-
forms were performed -by pentane extraction followed by
gas chromatography - electron capture detection (4). All
experiments were carried out at room temperature.  The
time-dependence of the haloform formation is shown in
Fig. 2-3. Chlorine was added to river water until a con-
centration of 1 mg/1 was reached. The haloforms were
analyzed after different periods. The reaction is completed
after about 1 hour.
Fig. 2-4 shows the results obtained when river water
is treated with different amounts of chlorine. Reaction
time 1 hour. At approximately 2 mg/1 a plateau level
is reached and no additional haloforms are produced
when higher amounts of chlorine are added.
Treating river water with chlorine dioxide results in
only minor formation of haloforms. This is, however, a
result of the small amount of chlorine present in the
chlorine dioxide solution used.
Thus chlorine does and chlorine dioxide does not produce
haloforms. To investigate the effect of a combination
of these, two experiments were carried out,  Firstly
                             1           •           ?
chlorine was added to river water at a concentration
of 1 mg/1. After 1 hour different amounts of chlorine
dioxide were added to the samples. After another hour
the samples were analyzed for their contents of. halo-
forms. Secondly different amounts of chlorine dioxide
were added to river water. After 1 hour chlorine was
added to a concentration of 1 mg/1. After an additional
hour the samples were analyzed for their contents of
haloforms. In these experiments pH was kept constant
at 6.97 ± O.O7 by a phosphate buffer. The results are

-------
 H9/I
101
                        - 488 -'
                       *-2
                    Fig.  2-3

                    The time  dependence of the
                    haloform  formation

                    1 . CHC13

                    2 • CHBrei-
       20    40    60    80
                                  Fig.  2-4

                                  Formation of haloforms from
                                  different amounts of chlorine
                                  2  m  CHBrCl
                                            2
      1    2
1    4    5    mi «2/|

-------
                         - 489 -. .:-x,v .


shown in Pig. 2-5. Smaller.amounts,of. halof OCTIS, *are .formed
when chlorine:dioxide is added before chlorine. ,It is
clear that the adding sequence,of chlorine and  chlorine
dioxide is important with respect to haloform, formation.
One might assume that chlorine dioxide degrades those
compounds which yield  haloforms upon chlorination .
                    1O   mg CI
Fig. 2-5
	=!	               /
Formation of haloforms from
combinations of chlorine and
chlorine dioxide
A « CHC1- ?  B « CHBrCl2
1 « Chlorine added before
    chlorine dioxide
2 = Chlorine dioxide added
    before chlorine
 3.   Chlorine dioxide in wood pulping industry

 Chlorine dioxide is used in w.ood pulping industry to .
 degrade and dissolve lignin residues in the sulfate.and  ,
 .sulfite pulps ("bleaching"). Until, now its use has been
 due mainly to pulp quality demand. In the future, however,
               of minimizing" the discharge of chlorinated

-------
                          - 490 -

products into lakes; .and 'streams will pjsobably result in
the increased use of chlorine dioxide as well as oxygen
in bleaching in place of chlorine and hypochlorite.
The use of chlorine dioxide in pulp bleaching is very
different from its use in  water purification. The
conditions are for example quite different as shown in
Table 3-1. It has, however, one important similarity.
The lignin in pulp is a similar material to the humics
present in many waters. Both are polymeric aromatic
materials.
TABLE 3-1
Comparison between conditions during chlorine
dioxide bleaching and during water purifica-
tion with chlorine dioxide.
                     Pulp bleaching
                               Water purification
pH
Temp.
Time
Cone, of added  C1O,
         Start  6, finish  3
         60-90°C
         30-180 min
         5 m mole/1; or., higher
6-8.5
0-20°C
extensive
<0.03 m mole/1
The most reactive positions in lignin and maybe also
in humics are the phenolic groups, 'The lignin phenolic
groups are oxidized to quinonic groups and muconic acid
groups (Fig. 3-1). Chlorine dioxide is reduced part-
ly to  chlorite  (1-electron-reduction) and partly to
hypochlorite (3-electron-reduction), •. The hypochlorite
formed then reacts either with chlorite  (see below)  or
with lignin.

-------
                         - 491  -
                          COOCH-
                         DOOM
Pig. 3-1
The reaction of phenolic
groups of lignin with
chlorine dioxide
The concentrations of inorganic chlorine compounds change
during bleaching as shown in Fig. 3-2. At first, chlorine
dioxide as well as chlorine, which is a companion of
chlorine dioxide in all technical chlorine dioxide pre-
parations, are rapidly consumed. The concentration of
chlorite increases as long as chlorine dioxide is
present but then decreases slowly. Thus chlorite is
actually the dominant bleaching agent. As shown in the
figure, chlorate is also formed. Its formation is a
useless by-product of the bleaching process since it
cannot react with lignin. It is also possible that in
water treatment the reaction of humics with chlorine
dioxide will follow the same pattern described by Fig 3-2,
The reaction between lignin and chlorite involves the
transformation of chlorite into chlorine dioxide which
then reacts with lignin. The hypochlorite formed by
this reaction  (see above) oxidizes chlorite into further
amounts of chlorine dioxide, which in their turn react
with lignin. The process is then a chain reaction.
Whether the reaction of chlorite in water with, for
example^ humics is a similar chain reaction is difficult
to say. The low reactant -concentrations are not benefi-

-------
                        - 492 -


cial for a chain reaction. Chlorite may in water treat-
ment be slowly transformed into chlorine dioxide by  an
oxide-reduction. If the reaction in the water is a chain
process/ however, its rate could vary widely depending
on the nature of the organic material, present.
                  o o
20      40      60
 TIME, min
 Fig.  3-2  The concentrations of inorganic chlorine compounds
           during chlorine dioxide bleaching of a. sulphate pulp
 Oxidation of phenols of different types to those pre-
 sent in lignin yields guinones, quinols and epoxides
 (Fig. 3-3).  The epoxides observed are rather unstable
 and are not detectable for some hours in water.

-------
                          -493 -
                                           HO   CH3
 Fig.  3—3  The reaction of mesitol with chlorine  dioxide

Chlorine dioxide reacts rather rapidly with double bonds
activated by  conjugation with benzene nucleus. For ex-
ample stilbene is oxidized to stilbene epoxide (Fig. 3-4)
The double bond of other stilbenes examined are split
by chlorine dioxide,


        a02+C6%-CH=CH-C6H5 —» CIO(?)+C6H5-CH-CH-C6H5
                                             Y

Fig. 3-4  One of the reactions of stilbene with
          chlorine dioxide

An inactivated double bond such as that present in methyl
oleate and cyclohexene reacts slowly with chlorine
dioxide. Oxidation involves formation of a-chloro-ke-
tones, carboxylic acids and traces of epoxides. The
chlorination products of the olefine (di-chloro com-
pounds and chlorohydrins)  are formed also by reaction
with chlorine dioxide.

-------
                        - 494,-

Mixtures of chlorine and chlorine dioxide are often
used in pulp bleaching. The ratio between the two
bleaching agents is then kept at a level which gives
a desired effect. For example, addition of a small
amount of chlorine dioxide to chlorine gives a more
selective attack on the lignin, which spares the
cellulose. Chlorine dioxide works as a radical sca-
venger hindering the radical attack of chlorine on
cellulose but not the non-radical attack on lignin.
Thus, it is also possible that in water purification
good results can be obtained by using mixtures of
chlorine and chlorine dioxide.
 4.   Chlorine and chlorine dioxide for water supply
     disinfection and taste and odour control;  experiences

 Disinfection

 Chlorine is  by far the most commonly used chemical for
 water disinfection.  Chlorine dioxide is used very often
 for taste and odour control,  but it is seldom  used*
 for disinfection,  even though it is a powerful disinfec-
 tant.  In fact in certain situations it may be  superior
 to  chlorine.  However,  more information is still needed
 concerning its potential use as a substitute to chlorine.

 Problems with the  analyses for chlorine arid chlorine
 dioxide

 There are several  methods of analyses for chlorine and
 chlorine dioxide which are well suited for water«-works.
 In  measuring chlorine  concentrations one usually deter-
 mines the total halogen concentration of the water.
 This so-called total chlorine concentration is regarded

-------
                            495 -
to be the sum of the free and combined chlorine consisting
of chloramines (mono-, di-, and trichloramine). During
analyses interactions with other chlorine compounds as
well as with bromine and iodine compounds can occur.
The total chlorine concentration can thus be written
     C1
        2 ' total
C10| + jci NH~  + ICl-compounds I  +
                +   Br,
     + |C13 N
     +  BrNH-
+ IBr-compounds|  +
                          +  ]Br3N   | +
                          +  |I-compounds
 Usually  the bromine  and  iodine  compounds  can  be  neglected.
 In  reality chlorine  in water  is in  equilibrium with
 hypochlorous  acid. At pH values exceeding 5,  which  is
 the case in all water treatment processes,  the real
 chlorine concentration is zero.  At  a  pH of 7.4 about
 80% of the total  chlorine is  in the form  of hypochlorous
 acid  (HC10)i,e. hypochlorite ion (CIO  ).
 This  can be seen  from the equilibrium curve.  Fig. 4-1 .
 It  is well known  that the hypochlorite ion has a very
 low disinfection  effect.
 Because  of the differences in disinfecting power of
 each  species  it is desirable  to distinguish them. In
 practice, however, the "chlorine residual"  is often
 measured by the o-toludin method or similar methods
 which do not distinguish between the different  species.
 Recently a method which  makes it possible to  distinguish
 between  chlorine  and chlorine dioxide has been developed
 in  Sweden (8).

-------
                          - 496 *•
       2  3  A  5  6  7
            pH VALUE
8 9 10
                                  Fig.  4-1
                                  Distribution of  chlorine
                                  hypochlorous acid and  hypo-
                                  chlorite  ion in  water  at
                                  different pH values
Behaviour of chlorine and chlorine dioxide as disinfec-
disinfectants
In order to prevent precipitation of calcium carbonate
in hard waters or to prevent corrosion in soft waters,
it is necessary to bring about equilibrium in the cal-
cium carbonate - carbonic acid system prior to the
distribution. In a very soft water, which is common in
Sweden this equilibrium cannot  be obtained with the
usual techniques. It is actually necessary to increase
the water hardness which is an expensive process. In
order to protect the pipes, and especially the copper
pipes, the pH value is normally kept as high as 8.5.
At this pH value the disinfection capacity of the water
is decreased, which may lead to unsatisfactory quality
in case of a secondary contamination.
The measurements of combined chlorine may not be satis-
factory for control of disinfection. Chloramines are
frequently used for disinfection in the distribution
system. It has been shown  (9) that the various combined
chlorine compounds may be different from one another
in capacity to inactivate bacteria or virus.

-------
                          - 497 -
•a
c
  o
  u
200

100
 60
 40

 20

 10
  6
  4

  2

  1
                                N
                               ^
                                I
                                     v
         1  2  4 6  10  20   6U100   400 1,000 ,
                     Tirne-spc              I
                                          !

 Fig. 4-2   Chlorination of Esch coli  (37°C,  pH 7),  using
            different forms of combined  chlorine  (9)


The equivalents  of  combined chlorine are plotted against
the time for  a  one  log reduction of the concentration
of live bacteria. The solid curve indicates  results ob-
tained by the use of  chloramine T; the  dashed  curve shows
results obtained by the use of NH-C1.
       5   10   15   20   25
          Applied Chlorine —ppm
                         30
                                  Fig.  4-3
                                  Free  residual'chlorination

-------
                         --498.'—'

The solid curves ' indicate '"the "relatibrfshlp, between-'the1 •''
applied concentration of free chlorine added to a con-
stant concentration of ammonia and the residuals found
of combined chlorine. The squares indicate the first
values at which residuals of free chlorine were also
demonstrable. The dotted curves represent  the rate of
reduction of live Esch coli at 37°C and pH,7. The time
in seconds for a one log unit reduction is plotted
against the applied concentration of chlorine. Identical
symbols are used for corresponding curves  for each of
the three experiments.
Most experiments with bacteria  have  been  made  either
with Escherichia coli or with bacteria described  as
belonging to the coliform  group.  However,  Ridenour and
Armbruster  (10) carried out  extensive  experiments with
different bacteria. For all  the organisms  they observed
similar results for chlorine or chlorine  dioxide^ They
concluded that E.  coli was more resistant  to chlorine
or chlorine dioxide than any of the  bacteria on their
list which could be considered  pathogenic.
At neutral pH values, parallel  experiments" by  a number of
researchers have shown chlorine  and chlorine dioxide,
at equal concentrations, to  be  about equally effective
against E. coli  (Bernarde, et al,  (11), Berndt (12),
and Ridenour and Armbruster,(1O)  ).  We have obtained
similar results in laboratory experiments  on coliform
group bacteria in  Gota Ji.lv raw  water (Gothenburg). The
effects of variations in pH  value were also examined.
The effectiveness  of chlorine was observed by  all
investigators to diminish  with  increasing  pH value of
the water (eg. Pair and Geyer,  (13) ).
With chlorine dioxide, however, there  has  been no such
agreement among different  investigators.  Ridenour and

-------
                         --49S- '•--.-

Armbruster  (1O)  observed that 'chlorine dioxide was at
least as  effective  at  pH 9.5  as at pH 7.  The experiments
were performed with E.  coli added to chlorine demand
free water.                 .
Bernarde  et  al.(11) found  that chlorine  dioxide was
more effective at pH 8.5 than at  pH 6.5.  A different rela-
tionship  was  observed  by Berndt  (1965). These experiments
were made using  raw water from the water-works at the
city of Lubeck.  The result  showed that chlorine as well
as  chlorine dioxide were more effective at lower pH
values  (pH  5) than  at  higher  pH values (pH 9.3).
Experiments with bacterial  spores have shown that chlo-
                                                f.
rine dioxide  is  more effective than chlorine when these
chemicals were  compared at equal  concentrations (Ridenour
and Armbruster  (1O), Berndt (12)  ).


Some comparative studies  (Gothenburg  1978) on  total
counts of bacteria at 22  in drinking waters disinfec-
ted by different techniques were performed. The  streak
plate method was used for growth tests  on Nutrient Agar
(Oxoid Type CM3), Tryptone Soya Agar  (Oxoid Type CM  131)
and Fe Pa medium according to Ferrer, et al. (.14) . The
first results show that on all the media used  growth
is more often obtained from samples taken ,from the dis-
tribution system of Uppsala (chlorine treatment) than
from the chlorine-dioxide treated water of Gothenburg.
Considerable data has been publishe'd  during the  past
twenty years showing the effectiveness  of chlorination
against different viruses under different conditions.
In general^ free chlorine in water effectively  inacti-
vated the different viruses which were  tested. Further—
more,chloramines or other combined chlorine forms were
found to be much less effective.   ,

-------
                         -*. soft --

Chlorine and chlorine dioxide have been compared for
effectiveness against poliovirus (Ridenour and Ingols,
1946).  At that time it was not possible to titrate a
virus concentration so 'Conveniently and accurately as
it is today. It appeared that chlorine dioxide added
to a virus suspension at pH 7 was more effective than
an equivalent dosage of hypochlorite.
The effects of changes 'in the pH value have also been
studied. In the range  pH 5 to pH 9  chlorination has
been consistently more effective in laboratory testing
at the lower pH values.
On the other hand, several investigators have shown
that chlorine dioxide inactivates viruses faster at
higher pH values than at lower -pH values. Cronier et
al. (15) showed a three-times faster 'inactivation of
poliovirus  1 at pH  9.O than at pH  7.O. Warriner  (16)
showed  the  same effect-on poliovirus  3. This effect was
more marked at higher dosages. Fig.; 4-4, Table 4-1.
When 0.2 mg C12/1 was added together  with O.5 mg C1O2/1
no improvement relative  to O.5 mg ClO^/l alone was
noticed.
Thus chlorine dioxide appears to be a better alterna-
tive from a microbiological point of  view than chlorine
since it is most effective in the pH  range in which
water is distributed.      -  •   - -
Except  for the generalizations about  the effect of pH
noted above, the results of many virus inactivation in-
vestigations have been .contradictory. The practical
application is limited because chlorination according
to the  same procedure may give* different results in
different waters  (Engelbrecht, et al,  (17) ). The diffi-
culty is enhanced because it is not practical to make
routine virological isolation tests in the manner that
routine bacteriological  testing can be carried out on

-------
  100-
 o
 z
 Ul
 ce.
   10-
       	.£*.§£_    .        r	
             0,2 mg/l ClOj
        10
                30

           TIME.min
                            60
                                    100
                                                 0,5 mg/l ClOj
                                                      10
                                                               15
                                            TIME.min
1,6mg/l  C102
                       I


                      o


                       1
Fig.4-4  Remaining virus as  a function of contact time at different dosages

           of  chlorine  dioxide at different pH  values  (16)

-------
T&BLE 4-1  Inactivation of poliovirus with chlorine dioxide at 20  C.
           The redox potential is measured against a caldmel electrode.
Parameter
Redox potential, mV
without virus
with virus
Amperometric titration of
chlorine-chlorine dioxide
residual, mg/1
chlorine
chlorine dioxide
chlorite
Cone, of surviving virus
(log ID5Q/0.1 ml)
without chlorine dioxide
with chlorine dioxide,
after 10-20 sec
after 1 min
after 2 min
after 5 min
after 1 5 min
after 60 min
Test No. 1
0.2 mg C102/l
pH 5.7 pH 7.0 pH 8.5

610 440 360
360 330 260



000
0.07 0.03 0.03
0.11 0.11 0.12


3.2 3.8 3.8

2.4 3.4 3.6
2.6 3.5 3.0
_ _- _
_
_ _
2.5 3.2 2.8
Test No. 2
0.5 mg C102/l
pH 5.6 pH 7.2 pH 8.5

670 660 580
630 580 270



0 - 0
0.27 - 0
0.12 - 0.41


2.8 2.5 3.5

1.8 2.2 1.6
1.4 1.6 0.6
1.2 0.8 O.8
0.8 0.5
0.4 0.5 0.2
0.4 0.5 0.4
Test No. 3
1 .6 mg C102/1
pH 5.6 pH 7.1;
•
720 705
700 690 '

•'•
' ~:
0.02 0.03-
1 .40 1 .41;
0.07 0.08J


2.0 2.5:
"
2.0 0.5-
1 .2 1 .3-
0 . 6 -0.4;
<-0.5 - „
— — ":•
4-
                                                                                               O
                                                                                               tsj

-------
                          - 503 -

                                                      !
both raw and treated water., .However,  investigations by
Lund (18, 19) have revealed a  relationship  between oxi-
dation potential and 'the  rate  of virus  inactivation.
This work has-' opened the  possibility  of regulating ,
chlorination, including chlorine dioxide treatment, for
the purpose of inactivating virus.

Figure 4-5 shows results  obtained for the inactivation
of poliovirus using chloramines  (9).  It shows  the  range
of potential measurements (with  a calomel reference elec-
trode)  which are likely to be  of interest in water   ;
treatment. Among 'the practical questions raised concerning
this procedure are the difficulties encountered in
measuring potentials and  the effectiveness  in  con-
trolling the bacteria.

The oxidation potentials  are plotted  against the time
in minutes i  "»ded for a reduction of  one log unit  of
active virus.
   700
   600
   500
   100
                 :Fig.' 4-5
                 Oxidation potentials obtained
                 by chlorination of poliovirus
                  (37°C,  pH 7)   (9)
           30
  60
Time—nun
                            120

-------
                          - 504 -

 The common denominator 'for1 disinfection chemicals' ds • • >v
 thus their ability to break through  the redox buffer
 capacity and thereafter change -the redox potential.
 The redox potential of a chlorine -solution is strongly
 dependent on the pH value/as can be  seen from Fig. 4-6.
 Th"e redox potential decreases with increasing pH and
 the possibility to break the redox buffer capacity is
 thus reduced. In soft waters, in which  it is  necessary
 from a corrosion point of view to have  a high pH value,
 a.  five-times higher chlorine concentration as in hard
 waters is required and still the disinfection effeciency
 cannot be guaranteed.
              goo.
                7,0
                                   ,CI2
                                   :edox=((pH)
                      0,5      1,0      £
                        CHLORINE RESIDUAL nig/l
8,0     9,0
 pH VALUE
10,0
Fig. 4-6  The redox potential as a function of the chlorine
          residual and  as  a function of the pH value for
          chlorine dioxide (—-),  chlorine (-8-) and
          chloramines  ( -  - -)

-------
                         -' 505. ,-..
Transformation, of£ ,chl
-------
                          - ,506


with  powdered activated  carbon,  ozone ,and, chlorine
dioxide,  showed a satisfactory  result by using a
mixture of chlorine and  chlorine dioxide, Fig. 5-1.
  uj lo-
  SS
                   r.VW.-.
                                    Fig.  5-1
                                    Results from taste control
                                    investigations
      LOT SAHD
      FILTER
ACTIVATED CARBON
3-5 9/»3  6-8 <
        EHim BEFORE TREATMENT
        [V^.v] AFTER TREATMENT
OZON
1.7 i
Subsequently  the raw water source - the  river  Gota
Hiv,- was  contaminated by wastes and this,  of course,
affected  the  taste and odour. The raw, water  intake was
rebuilt and the river water was.pumped to  a  natural
lake acting as  a reservoir..Together  with  increased
river pollution control, the situation was markedly
improved. An  additional problem then  arose occasionally
- during  spring and autumn a mass production of algae in
the lake  resulted in taste and odour  in  the  water. On
such occasions  chlorine dioxide treatment  alone was
insufficient  and therefore granular activated carbon
was introduced  and has been used for  nearly  10 years.

-------
                          - 5O7 -
In the taste" and odour  investigations  in Gothenburg
it was noted in agreement with  others(1)  that it is
possible to have a higher "chlorine residual" with
chlorine dioxide than with  chlorine without producing
a chlorinous taste  (Fig. 5-2).
                                   Fig.  5-2
                                   Taste intensity as a function
                                   of the "chlorine residual"
                                   from the different methods of
                                   of treatment
          0.05    0.010    0.015   0.020
         CHLORINE RESIDUAL, mgj\
We conclude  the  following:  chlorine dioxide can im-
prove the taste  and odour and does not form haloforms.
Furthermore, ;chlorine dioxide is an excellent disin-
fectant in the distribution system and this facili-
tates the/supply of a safe  and potable water.

-------
                      - 508 -
(1)  U.S.  Environmental Protection Agency
    Ozone,  Chlorine Dioxide and Chioramdnes as Alternatives
    to Chlorine for Disinfection of Drinking Water
    State-Of-The-Art, Nov. 1977

(2)  GORDON, G., KIEFFER, R.G., ROSENBLATT, D.H.
    The Chemistry of Chlorine Dioxide
    Progress in inorganic Chemistry, Wiley-Interscience,
    New York 15 (1972), 259-286

(3)  GROB,  K.r  ZtiRCHER, F.
    Stripping  of trace organic substances from water,
    equipment  and procedure
    J. of  Chromatography 112 (1976) , 285

(4)  EKLUND, G., JOSEFSSON, B., ROOS, C.
    Determination of Volatile Halogenated Hydrocarbons in
    Tap Water, Seawater and Industrial Effluents by Glass
    Capillary  Gas Chromatography and Electron Capture
    Detection
    J. High Res. Chrom. 1_ (1978), 34-4O

(5)  EKLUND, G., JOSEFSSON, B., ROOS, C.
    Trace  Analysis of Volatile Organic Substances in
    Goteborg Municipal Drinking Water
    Vatten 31  (1978), No. 3

(6)  MORRIS, J.C.
    The Chemistry of Aqueous Chlorine in Relation to
    Water  Chlorination
    Water  Chlorination, Environmental Impact and Health
    Effects
    Ann Arbor  Science, Ann Arbor _1_ (1978) , 21-35

(7)  ROOK,  J.
    Chlorination Reactions of Fulvic Acids in Natural Waters
    Environ. Sci.  Technol. 1_1_ (1977), 5, 478-482

(8)  ISACSSON,  U.,  WETTERMARK, G.
    Selective  analysis of chlorine (hypochlorous acid)  and
    chlorine dioxide using chemiluminescence
    Anal.  Lett. r\_ (1978), 13-25

(9)  KJELLANDER, I.,  LUND, E.
    Sensitivity of Esch Coli and Poliovirus to Different
    Forms  of Combined Chlorine
    J. AWWA 57 (1965),893

-------
                        - 509 -
(TO)  RIDENOUR,  G.M.,  ARMBRUSTER, E.H,
     Bactericidal Effect of Chlorine Dioxide
     J.  AWWA 41_ (1949) ,  537

(11)  BERNARDE,  M.A.,  ISRAEL, B.M., OLIVIERI, V.P.,
     GRANSTROM, M.L.
     Efficiency of Chlorine Dioxide as a Bactericide
     Applied Microbiology 13 (1965), 776

(12)  BERNDT, H.
     Untersuchungen zur  Wasseraufbereitung und Wasser-
     desinfektion mit Chloridoxyd, insbesondere zu Fragen
     der pH-Wert-Abhanaigkeit und der Chloritriickbildung
     Arch.  Hyg. 145 (1965), 1O

(13)  FAIR,  G.M., GEYER,  J.C., OKUN, D.A.
     Water  and  Wastewater Engineering, Wiley 2^ (1968), 31-18

(14)  FERRER, E.B., STAPERT, E.M., SOKOLSKI, W.T.
     A Medium for Improved Recovery of Bacteria from
     Water
     Can. J. Microbiol.  9^ (1963), 42O-422

(15)  CRONIER, S.,  SCARPING, P.V., ZINK, M.L., HOFF, J.C.
     Destruction by Chlorine Dioxide of Viruses and
     Bacteria in Water
     Abstr.  Ann. Meeting American Soc. for Microbiol. (1977)

(16)  WARRINER,  R.
     Inactivation of Poliovirus with Chlorine Dioxide
     Vatten, Arg 23_ (1967), 284-29O

(17)  ENGELBRECHT,  R.S.,  WEBER,  M.J., SCHMIDT, C.A., SALTER, B.-L.
     Virus  Sensitivity to Chlorine Disinfection of Water Samples
     EPA-600/2-78-123

(18)  LUND,  E.
     Inactivation of Poliomyelitis Virus by Chlorination at
     Different  Oxidation Potentials
     Arch.  Ges. Virusforsch. 11 (1961), 33O

(19)  LUND,  E.
     Effect of  pH on the Oxidative Inactivation of
     Poliovirus
     Arch.  Ges, Virusforsch. 12 (1963, 1

(2O)  Investigation of Different Methods for Taste and Odour
     Improvemen t
     Chalmers Univ. of Tech., Dept. of Water Supply and
     Sewerage,  Series B  64 (1964) , 1

-------
                          - 510 T- , :.-


REMOVAL OF ORGANIC MATTER FROM WATER BY UV AND
HYDROGEN PEROXIDE

L. Berglind, E. Gjessing and E. Skipperud Johansen
1.  Introduction
In 1966 Armstrong and coworkers  (1) introduced a method
for the removal of organic matter from samples of water
to be analyzed on total phorphorus. Samples of alcohols,
carbohydrates, organic acids and humic acid in aqueous
solutions were exposed to ultraviolet (UV) irradiation
in the presence of oxygen. It was found that the content
of organic compounds was reduced below detectable levels.
The only final product which was registered was carbon
dioxide, CO2.

It has been noticed that sunlight may induce both fading
of the colour and reduction of organic matter in water  (2,3),

Elimination of organic pesticides has also been carried out
by photo-induced oxidation (4,5) as well as photo-induced
decomposition of formic acid and dodecyl benzene sulfonate
in aqueous solutions (6,7) . Photo-oxidation of refractory
organic compounds in municipal wastewater has been given
increased attention during the past years  (8,9). The process
of oxidation is usually very complex. Some highly reactive
intermediates are involved. One of these is the hydroxyl
radical OH*, which is formed during light absorption (7,1O,
11). The rate of oxidation is primarily limited to the yield
of OH*. For the purpose of water purification, the method of
photo-oxidation requires an excess of OH.' One way to obtain this
is by adding hydrogen peroxide  (H2O2) to the system. It
is known that UV irradiation may induce breakdown of H2O2
into hydroxyl radicals and thereby accelerate the process
of oxidation (1,11,12).

-------
                         -  51 1 '-
2.  Earlier Results                                 •
In the late sixties we suggested that some 20 % of the
observed reduction of humus colour in lakes and rivers
may partly be due to natural UV radiation and that this
principle should be considered as an alternative method
for the purification of surface water based on artificial
UV (13). Laboratory experiments showed that humus in
water, which is persistent soil originating organics,
were completely mineralized by a combination of UV radi-
ation and hydrogen peroxide. Several other oxydants were
also tested together with the UV, such as O3, O- and air.
However, H-O- appears to be outstanding with respect to
these effects. Some results 'are summarized in Table 1.
  TABLE 1;   Effect of UV radiation of aquatic humus.
            Per-cent reduction of colour (or increase(+))
            and organic carbon by different "doses"  of
            UV radiation under various conditions.
"Chemical"
added
Air
N2
Air
Air
H2°2
Cont
Ba02


(PH 7)
(pH 3)

.aeration
+ air
Col
Minutes of
1
+ 2
+ 1
+ 12
+ 5
45
6
11
5
+ 6
+ 2
+ 1O +
4
97
O
8
our
Radiation
20
13
11
8
14
98
21
10
60
41
16
25
35
99
. -
-
' Organic
Minutes
1
6
O
6
0
43
2
9
5
13
5
9
3
73
2
1 1
Carbon
of Radiation
2O
23
11
16
14
92
10
16
6O
41
26
19
15
100
-
—

-------
                         -  512

 Essentially the  same procedure as that used for humus was
 also used on an  aqueous solution of the polyaromatic
 hydrocarbon, 3.4-Benzopyrene (B(a)P)  : 99 % removal from a
 100 yg/1 solution  resulted.

 It is strongly believed that the combination of UV and
 H000, being so effective  as  to mineralize humus (and
  £* «&>
 B(a)P), might be a potential method for the removal of
 organics from water. However,  is it practical and what
 will it cost? The  purpose  of the present work is to gather
 more information around these two questions.
 3,  Recent Results
 In a set-up as illustrated  in  Figure 1  different kinds of
 organic matter were recirculated for several hours. Samples
 were collected periodically. In Table 2 the tested organics
 and chemicals are listed.
      Wedeco
      E/10-2
               uv—
                    uv
210W
                    Pump-
                                 ^jfJA.
                                xT^xxxxvxxxx/v
                                        ,»
                              100 liter
                              Cooler
Fig. 1  UV-radiation plant. The radiation  unit  consists of a
        quartz tube surrounded by  6x3O W U¥ lamps  (254 nm).
        The hydrogen peroxide is added to  the water  in the
        tank immediately before start. The pumping rate was
        O.8 I/sec.

-------
                        - 513 -
TABLE 2;  The water samples and chemicals tested in the
          UV pilot plant

1 . Humus water
2 . Humus water
3 . 3 , 4-Benzopyrene
4. 2-Methylisoborneol (MIB)
5 . Chlorof orm/bromo-
dichloromethan (BDCM)
Colour
mg P-t/1
109
27
-
-
-
TOG
mg C/l
14,5
4,2
-
-
-
Cond.
yS/cm
31
32
-
-
-
pH
4,9
6,2
-
-
-
Cone.
yg/1
_
-
65
1
1 OO/1OO
    Humus
Eight 50-litre aliquotes of humus sample No. 1 were circu-
lated for four hours (O.8 I/sec) in the system illustrated
in Fig. 1, with different amounts of 35 % H202 added,
ranging between O and 5O ml/1. Water samples were collected
after 3O, 6O, 12O, 18O and 24O minutes and analyzed on
colour and TOG. The relationship between colour- and TOG
removal and the amount of H-O., added is illustrated in
                           ^ £
Fig. 2. 12O minutes radiation is considered.
Humus sample No. 2 showed the same H-0., response with regard
                                    ^ £
to humus removal, namely: maximum effect with O.2 ml of
35 % H20? per litre. It is interesting and important to note
that higher concentrations of H?O2 did not result in a better
removal of organic matter and that the amount of H2O? appears
to be independent of the amount of humus.

-------
                            - '514 -
 WO
     10
          HOC
Radiation time  120 min
Q = 0.81/Sec,
Energy used:420Wh
Volume of sample : 501
           \COLOUR
                          '.0
                        ml 35% H20,/I water
                                             20
Fig. 2  The  relationship between TOC colour removal and the
        amount of hydrogen  peroxide (H7O7) added at a fixed
        11UV  dose"


 In Figure 3 the "UV dose"-response relationship regarding

 colour removal with a fixed  ^2°2 dose  ^°*2 ml  35 % H2°2/1
 is plotted.  Fig. 4 shows the corresponding reduction of
 total organic carbon.
 100-
                                       Fig.  3            .
                                       Reduction of humus colour
                                       with  different "UV doses";
                                       O.2. ml  35 % H2O2/1 added.
                                       The low-coloured water  is
                                       also  run with low flux
                                       (O.2  I/sec). It was noticed
                                       that  the colour of the  samp-
                                       les collected during the run
                                       was reduced during 1-2  days
                                       of  storage
      •5  30     60
        Minutes ol UV- Radiation
                         120

-------
                          -'515 -

                                Fig.
                                Reduction of total organic  carbon
                                (TOG)  in two different humus  samp-
                                les -with different "UV doses" and
                                O.2 ml 35 % H2Q2/1 added. The TOC
                                analyses are to some extent un-
                                certain, probably due to some
                                interfering substances in the
                                samples.
      30 SO     120    180

      M mules of UV-Radralion
Figure 5  illustrates the percent  reduction (with time) of

colour and  TOC.
                           Fig. .5

                           Percent  reduction of colour and
                           organic  carbon in two different
                           humus samples with different "UV
                           doses" and  with O.2 ml 35 % H2O2/1
                           added.
       30   60     120
      Minutes of UV-Radiolion
180

-------
                        - 516 T-  •---:. ,


In the brief discussion below>the carbemi results,are omitted
because at present we are uncertain as to whether the routine
method used  (wet oxydation in glass ampulla and IR deter-
mination of the resulting CC^) is appropriate for these
samples. Considering Figure 3, it appears that the same
"UV-dose"  (and amount of ^02) removes,  in the beginning of
the process, twice as much colour from the highly-coloured
water (1O9 mg Pt/1) as from that of low  colour (27 mg Pt/1),

The results suggest that the apparent linear colour reduction
is levelling off when the residual colour is in the range of
10 mg Pt/1.

Figure 3 shows that there is only need for twice as much
energy to reduce the "1O9-coloured" water by 95 % as that for
"27-coloured" water. All this suggests that, under the con-
ditions outlined, the method appears to  be relatively
most effective on highly coloured waters.
    Organic Chemicals in Water
1OO litres of distilled water, containing the concentrations
and chemicals listed in Table 2, were recirculated for 1-4
hours. Samples were taken at different time intervals and
analyzed on the chemical substance concerned. The results are
given in Table 3.               ...

The results are also illustrated in Fig. 6. In general,
these results show more than 9O % reduction of the chemicals
treated with UV radiation for 2 hours. MIBr which is one of
the chemicals responsible for a characteristic soil smell and
-taste, which may appear in drinking water reservoirs, is
removed below detectable limits within 3O minutes.

-------
                        - 517 -
 TABLE 3;   Removal of organic chemicals from water with
           UV radiation and hydrogen peroxide
without H~0~
£. Z

1 O —
>-H X
P 4J
«J ft
g 05 ra
0 65(0)
30
,60
120 42(29)
24O
yg/1
e
IH -
0 "R
o a
-H 0
fi Q
U ffl
1OO(O) 1OO(O)
- -
92(8) 91(9)
42(58) 75(25)
- -
with O.1 ml 35 % H2O/1
1 a
^ o
^j Hi
^x O

-------
                        -  518 •-'- ' '-  '"

4.  Cost Estimates
Except for MIB and the low humus-coloured water, it appears
from the results that,-, with a H2O2 dose of O.1 - O.2 ml
35 %/l and at'12O minutes of radiation, a 9O - 95 %
removal is achieved.

Considering 5O % removal and the following price estimates:
Electric energy
H202 (35 %)

UV-lamps
Pilot plant
Lifetime of UV-lamps
   1O.OOO hours
1O % interest of
   Plant cost
Nkr.  2.2OO.
Nkr. 15.OOO.
               Nkr/kWh  O.15  (O.O3)
               Nkr/1    3.85  (0.70)
               Nkr/h
               Nkr/h
0.22 (0.04)
0.17 (0.03)
In Table 4 the resulting costs are shown.
TABLE  4;  Estimated  cost  of  water with 5O  %  removal  of the
          organic matter. All  figures  are  in Nkr  (or $)
          per m3.
Sample
1
2
3
4
5
6
Humus 1
Humus 2
B(a)P
MIB
Chloroform
BDCM
Mean %
H2°2
.8
.8
.4
.4
.4
.4
14
El.
Energy
.2
.4
.2
.1
.4
.2
6
Lamp Plant
1.5 1.1
2.7 2.1
1 .5 1 .1
.6 0.4
2.5 1 .9
1.7 1.3
46 34
Total
Nkr. $
3.6
6 .O
3.4
1 .5
5.2
3.6
1OO
.65
1 .10
.62
.27
.94
.65
1OO

-------
                        -SIB -,,,' ...


It appears from the figures given in Table 4 that the main
costs(8O %) are connected with the equipment, whereas the
energy and hydrogen peroxyde expenses are in the range of
1 Nkr/m  (* $-18/m ). For a 95 % removal, the costs are from
4O- % to several hundred per cent higher.            ;
5.  General Discussion
Laboratory experiments have suggested that organic matter
in water is quantitatively converted to C07 and H9O by
                                          *-n      £*
UV radiation and hydrogen peroxide. For drinking water
purposes this seems ideal: no foreign chemicals added and
no residual-component problems I However, the present pre-
liminary experiments suggest that the costs are high.
Nevertheless, the method should be further investigated.
It is important to emphasize that the mechanisms behind
this mineralization of aquatic organics are not clearly
understood and that an increased efficiency may be
achieved by a combined use of theoretical consideration
and recent research and practical experiments.

We find it reasonable that the work is continued along
the following lines, experimenting on:

   1.  Action, of cataysts                        ;  •
   2.  Combination of chemicals
   3.  The most effective wave length
   4.  Increased efficiency of DV-lamp
   5.  Most effective way of dosing the active .oxygen
   6.  Identification and biological evaluation of
       residuals   ,     ' •        "

-------
                        -  520 -   '-. 7

It has been stated that some reactive intermediates are
involved which are formed by the action of light. Hydroxyl
radicals are, as mentioned in the introduction, suggested
as such an intermediate (7,10,11).

Zepp and coworkers (14) are .using the term "singlet oxygen",
stating that this may be generated in water by light energy
and oxidize a variety of organic substances. They suggest,
as Kautsky, that the most likely mechanism for the oxygena-
tion in the environment is that light energy adsorbed by a
sensitizer is transferred to ground-state oxygen to form
singlet oxygen, which in turn reacts with the organic sub-
stance or "acceptor" to form a peroxide. In their experiments
they demonstrate the importance o'f the presence of "singlet
oxygen sensitizer". The results outlined above indicate  a
catalyzing or sensitizing action of some constituents
in the humus sample, and a combination of chemicals may
accelerate the mineralization processes.

In the work of Zepp and coworkers  (14) the 366-nm line
is used. In the work presented above, the UV source is a
lamp which is claimed to have a sharp maximum at 254 nm.
It is reasonable to assume that the wave length of the
radiation will greatly influence the results. The UV-
lamps commercially available have a relatively low effect.
Those used in the present work are reported to give less
than 25 % of the energy used..Hpweyer, according to the
manufacturer, this will be improved in the future.

The hydrogen peroxide is added to the test water immedi-
ately before the start of the experiment. It is interesting
to note that the most effective H2O2-dose appears to be
independent of the concentration of the organic matter
present. The fate of the I^O^ added to the polluted water
during the 2-4 hours lasting experiment is, however, not

-------
                        - 521 --• .*;•••  -


completely known.  It  is  possible  that  the mineralizing
effect may be  improved by  applying the active  oxygen con-
tinuously or stepwise during-the  run .

The  "ghost" behind drinking  water and  drinking water
treatment today is the organic  residuals. The  engagement
with regard to chlorination  of  water containing organics
in general and humus  in  particular is  considerable  through-
out  the whole  world.  This  'Concern about the  residuals and
their potential toxicity should also be applied to  both
natural and artificial UV  radiation. In the  present work
some analyses  on the  residuals  .after UV-H2O2 treatment
are  in progress. At any  rate, .this type of research is
essential in future, work with this principle of drinking
water treatment.      ••.-..•'
6.  Conclusion          . .  ;
There is need for a method by which it is possible to
eliminate organic matter from 'drinking water. Combined
use of UV radiation and active oxygen appears in prin-
ciple to be a promising method in this respect.

Experimental results from a relatively small UV radiation
plant (necessary residence time 5-2O h/m )  suggest that
the price of this treatment Is" high. The mechanisms involved
are, however, not completely understood and the efficiency
of the method may be improved by altering the conditions,
studying the effect

-  at different wave lengths"
   of a combination of chemicals
   of different ways of applying the H-O, of modern
   UV-lamps and.of different catalysts.

-------
                      - 522 .-
 (1)  ARMSTRONG, F.A.J., WILLIAMS, P.M., STRICKLAND J.D.H.
     Photooxidation of Organic Matter in Sea Water by
     Ultraviolet Radiation, Analytical and Other Application
     Nature 211 (1966), 5O48, 481-483

 (2)  BEATTIE,  J.,  BRICKER, C., GARVIN, D.
     Photolytic Determination of Trace Amounts of Organic
     Materials in Water
     Anal.  Chem. 33 (1961), 189O-92

 (3)  GJESSING, E.T., SAMDAL, J.E.
     Humic  Substances in Water and the Effects' of .
     Impoundment
     J.  AWWA j5O (1968), 4, 451-454

 (4)  HENDERSEN, G.L., CROSBY, D.G.
     Photodecoraposition of Dieldrin Residuals in Water
     Bull.  Environ. Contain. Toxicol.  _3 (1968), 131-134

 (5)  BOLLER, C.D., EDGERLY, E. jr.
     Photochemical Degradation of Refractory Organic
     Compounds
     J.  WPCP 40 (1968), 546-556 ;

 (6)  MATSUURA, T., SMITH, J.M./.'
     Photodecomposition Kinetics of Formic Acid in
     Aqueous Solution
     AIChE  J.  16 (1970), 1064-1071

 (7)  MATSUURA, T., SMITH, J.M.
     Kinetics  of Photodecomposition of Dodecyl Benzene
     Sulfonate
     Ind. Eng. Chem.Fundam. 9_ (197O), 252-26O

 (8)  SCHORR, V., BOVAL, B., HANCIL, ¥., SMITH, J.M.
     Photooxidation Kinetics of Organic Pollutants in
     Municipal Waste Water
     Ind. Eng. Chem. Fundam. 10 (1971), 4, 5O9-515

 (9)  MAUK,  C.E., PRENGLE, W.    .
     Ozone  with Ultraviolet Light Provides Improved Chemical
     Oxidation of Refractory Crganics
     Pollut. Engineer.  8^ (1976), 42-43

(1O)  FOOTE, C.S.
     Photosensitized Oxidation and Singlet Oxygen Conse-
     quences in Biological Systems
     Free Radicals in Biology II,  -Academic Press, New York
     (1976), 85-133

-------
                         523  -
(11)  KOUBEK,  E.
     Oxidation of Refractory Organics in Aqueous Waste
     Streams  by Hydrogen  Peroxide and Ultraviolet Light
     US Patent Nr. 4,  012, 321  (1977)

(12)  SCHENK,  G.O.
     Institut fur Strahlenchemie,  Max Planck-Institut fur
     Kohlenforschung,  Mxilheim,  personal communication (1977)

(13)  GJESSING, E.T.
     Influence of Ultra-Violet  Radiation on Aquatic Humus
     Vatten 2_ (197O),  144-145

(14)  ZEPP,  R.G.,  WOLFE,  N.L., BAUGHMAN, G.L., HOLLIS, G.L.
     Singlet  Oxygen in Natural  Waters
     Nature 267  (1977),  421-423

-------
                          -  524.-
  i-
ANODIC OXIDATION AS A PROCESS STEP IN THE TREATMENT ,OF
BACTERIALLY CONTAMINATED WATER

N, Kirmaier
Extensive scientific studies in recent years have been, able
to demonstrate the effectiveness of the bacterial decontam-
ination of water by anodic oxidation  [1-3].

The original hopes of being able to use anodic oxidation
specifically in electrosynthetic processes were disappointed
in most cases on account of the formation of undesirable by-
products.  Nevertheless, there was a positive outcome for the
treatment of water: microbial constituents and oxidizable
chemical substances present in the water are oxidized unspeci-
fically.  In this way microorganisms are inactivated and the
chemical loading of water is degraded.

The electrochemical action of anodic oxidation in water treat-
ment can be arranged under two group headings:

a)       the actual step-through reaction at the phase
         boundary between the electrode and the electrolyte,,

b)       the secondary reaction @.ue to reaction products)
         within the solution.

Since as far as we know cathodic reactions do not cause
bacterial inactivation, and chemical reactions cannot.be
solely responsible for the inactivation performance of the
anodic oxidation reaction cells, the actual oxidation of the
bacterial  substrate of microbes must take place on the
electrode surface or on the boundary region of the anode. The
basic course of the reaction can thus be described by the
general equation:

-------
                           - 525--
Ox  (I) + Red  (II) ^	> Red  (I)  +.Ox  (II)

where Red  (II) stands  for an  active bacterium  and-Red  (I)
for an inactivated bacterium  [4].

If the bacterium were  inactivated by active  oxygen,  the
reaction would depend  on processes of diffusion  through  the
bacterial cell wall and on  further partial steps of  the
reaction in the cytoplasm and the cell nucleus up  to the
  .  •               '                                      >
oxidative attack on the DNA or  on messenger  RNA  [4].

Studies were carried out with waters  (mains water,  surface
water) contaminated with viruses and bacteria.   Fig. 1 shows
the inactivation dynamics for the polio virus  in Munich  mains
water, and Fig. 2 the  inactivation dynamics  for  the  ECBO
virus [5].  Only a relatively slight reduction of  the infec-
tiousness could be achieved at  low current densities, while
                                      2
with current densities of over  5 mA/cm  a complete inactiv-
ation of the test viruses used  is possible  [5].
Other problem microorganisms  (in concentrations  of  up  to
10  cells/ml) '(gram-negative  bacilli, aerobic  spore-formers,
yeasts, mycelial fungi, and gram-positive cocci), can likewise
,be fully inactivated.

-------
                         -  526.--
                                      * Water  400  pS

                                      fc Water  800  pS
                           zoo
                                       300
  Current, mA
Fig. 1  Inactivation  dynamics  of  the polio virus in Munich
        water,  sterile  filtration?  through-flow 7.5 ml/sec
        at  1O-12 C; conductivity  adjusted with sodium chloride
           H

           <
           o
           tn
           O
           r-l
           C
           fl)
           4J
           C
           O
           o
           M
           •H
                                       * Water  400  pS

                                       O Water  800  pS
             Current,
Fig. 2  Inactivation dynamics for the ECBO virus  (5)

-------
                          -  527' -'-
.(1.) Reis, A.
    Die Anodisclxe  Oxidation als Inaktivator pathogener
    Substanzen und prozesse             ,      .  '  .. • "
    Klin. Wschr. .11951),  484

 (2) REIS, A., KIRMAIER, N.
    Anoden-Warturiscrubber zur Luftreinigxing
    ,G-I~T. Fachz.  Lab.  22 (1978)., 197

 (3) REIS, A.
    Ingenieuraufgaben in  der Medizin
    Biomed. Technik,  Oldenbourg-Verlag, Mtinchen/Wien
    2. Aufl.  (1977)

 (4) EIBL, V.
    Dissertation,  TU  Miinchen (in preparation)

 (5) MA.HNEL, H.
    Virusinaktivierung. in Wasser durch Anodische
    Oxidation                       •   -    :.    .  .  . .
    Zbl. Bakt. Hyg.,  Orig.  B 166 (1978), 542

-------
                          - 528- - ,


EXPERIENCE WITH POTASSIUM  PERMANGANATE
K. K6tter
1.       Preliminary remarks
When a conference considers the problem of oxidation processes
and in so doing gives emphasis to chlorine and ozone,potassium
permanganate should not be omitted from the discussion.  This
oxidizing agent undoubtedly does not rank among the primary
agents used in water treatment, but it does have some inter-
esting properties.  Reference should therefore also be made
to these, even though there are already detailed publications
on potassium permanganate, on its chemical reactions, and on
i-ts possible uses in water treatment [1,2, etc.].

This short experience report will be limited to the use of
potassium permanganate in recent years on Gelsen water.
Before this, however, a brief mention of the commercial situ-
ation of potassium permanganate: a major manufacturer of this
compound estimates that potassium permanganate is currently
in use at more,than 250 waterworks in North America and at
more than 30 in Europe.  For three years there has been a
German Industrial Standard for this product in West Germany
[3].

2.       Use of potassium permanganate to prevent the growth
         of filamentous algae in infiltration reservoirs
The artificial enrichment of ground water with slow sand
filtration with the use of large infiltration reservoirs is
regarded as the principal process step in the Ruhr valley
and in other large water treatment plants.  Mass growth of
algae and its adverse effects on this method of obtaining
water and on the water quality have been known for a long
time.  In the spring the microscopic plankton algae predom-
inate, in the summer it is the macroscopic filamentous algae

-------




                               *
Fig. 1  Mass growth of algae in infiltration reservoirs

-------
                                                                                              Ul
                                                                                              U)
                                                                                              O
Fig. 2  Swimming filamentous algae as in Fig.  1, photographed  from a

        closer distance

-------
                          -  531 -


(Figs. 1 and 2).  In the infiltration reservoirs the algae
find conditions favourable for their proliferation,- namely a
slowing down of the flow rate, favourable illumination
conditions, and a steady supply of food.  Since the algae
constantly release harmful metabolic products and on dying
give off harmful incorporated substances, it is better to
prevent the algal growth than to try and destroy it later,
especially as relatively few chemical controlling agents are
permissible in the treatment of drinking water.

The action of potassium permanganate on the growth of fila-
mentous algae was studied at Gelsenwasser AG in large-scale
trials lasting several years, performed in varioiis water-
treatment plants on the Ruhr and in reservoirs  [4,5].  An
addition of 1 to 1.5 g/m  of,potassium permanganate 'to the
inlets into the infiltration reservoirs largely prevents the
growth of filamentous algae, while in a parallel trial with
untreated tanks dense .mats of Hydrodictyon were produced.

The action mechanisms of the potassium permanganate remain
largely unexplained.  They could be based on its oxidizing
properties or on other properties toxic to the algae.

The considerable reduction of .the water's transparency to
light undoubtedly plays -an important part.  Certain wave-
lengths are absorbed to the extent of up to 99%.  Therefore
the light conditions at the bottom of the reservoir are. no
longer adequate for the development' of the algae.

To stop the growth of filamentous algae the KMnO^ addition
must be commenced promptly in the spring.  When already
formed, algae swimming,to the surface are little affected and
can reproduce on the well illuminated surface of the water.
In the light of the positive experience, potassium perman-
ganate dosage plants have in the -meantime been installed in
all the Ruhr waterworks of Gelsenwasser AG, and since the

-------
                          -  532  -

spring of 1977, with; .the. except ion of ..some,, remotely situated  >
reservoirs, all the artificially Infiltrating water in these
Ruhr waterworks has been first, treated with potassium per-
manganate.

3.        Action of potassium permanganate  on plankton algae
          in infiltration  reservoirs  •                     .  .
The favourable action of  potassium permanganate unfortunately
extends only to the filamentous algae, and not to  the plankton
algae.  This may be partly  due  to the  fact that these algal
forms develop  in free water bulks, ~ where  because  of the
small layer thickness the light absorption of the  permanganate
has less  effect than at the bottom of  a reservoir.

In the summer of 1973 an  attempt was made  at Halingen water-
works to  prevent the growth of  plankton algae by increasing
                                                    3
the amount of potassium permanganate added to 4 g/m .  The
treated water  - in some  reservoirs "also receiving 0.3 to
       3
0.6 g/m   of copper sulphate -   assumed an  intense  violet
coloration.  Interestingly  enough, these additions had no
clearly positive or negative effects,  either on the plankton
algae or  on the biological  purification performance- of the
slow sand filter.  Nor could any harm  to the zooplankton be
established.

On the over-all evaluation  of potassium permanganate as an
anti-algal agent in water treatment it must be said that  it
is sadly  less effective against the plankton algae, i.e.
against the algal species that  at times impair the seepage.
However,  the filamentous  algae, whbs'e  biomass production  far
outweighs  that of the plankton algae'  and  that can give rise
to a considerable impairment of odour, can be controlled by
potassium permanganate and  so far no disadvantages of any
kind have been discovered.  This on the whole positive balance
has lead  to the decision  to add potassium  permanganate contin-
uously to the raw water between March  and  September.

-------
                          - 533 - -

4. '• •   ' '• Potassium permanganate* as" 'an agent-for the oxidation
         of dissolved manganese in an oxygen-containing water
The following partial report concerns the water treatment of
the Stiepel waterworks of Wasserbeschaffung Mittlere Ruhr
GmbH (WMR).  The waterworks are situated in the Ruhr valley
and are operated with Gelsen water.  Up to 2500 m /h of true
ground water and Ruhr baiiK filtrate are used.  Tnere is no
artificial ground water enrichment via infiltration reservoirs
or absorption wells.  The manganese contents in the drinking
water rose in the 'sixties to  5 - 8 mmole/m   (0.25 - 0.40 mg/1)
of Mn> although the oxygen content in the natural ground water
and ground water enriched by bank filtration, i.e. in the
mixture of raw waters of different origins, never fell below
3 mg C>2/1. By shutting down the most severely affected wells
a temporary slight improvement of the mixed water quality
could be obtained, but this measure was not regarded as a
satisfactory long-term solution.  The only promising counter-
measure was the erection and setting in operation of a deman.—
ganization plant.

In collaboration with drinking water treatment companies and
with the Engler-Bunte Institute of Karlsruhe University,
various treatment trials were carried out with the particular
aim of clarifying whether a biological or autocatalytic
manganese oxidation takes place in water containing  sufficient
oxygen or whether the use of oxidizing agents would be necess-
ary.  At first biological oxidation was favoured, because this
had proved to be effective and reliable in the reservoirs
used at the Haltern waterworks  (up to 50 m/h filtration ve- •
locity and 10,000 m/h throughput).  At the Stiepel waterworks,
on the other hand, a filtration preceded by chemical oxidation
gave better results.  Surprisingly, potassium permanganate
proved to be a more effective oxidizing agent than chlorine.
For this reason, and because without preliminary chlorination
the formation of organic chlorine compounds does not come

-------
                          T  534  -
into consider at ion",™!" "plant" "for" "long-term potassium permanganate^
addition was set up and  put into  operation.   The construction
and the operating results  are  presented  in Fig.  3 and in the
following list:
KMnO. addition
   : a constant 0.5 g/m , i.e. referred
     to the fluctuating manganese concen-
     tration in the filter inflow 0.9 to
     2.1 times the stoichiometric amount.
Filter bed
  70 cm of pumice,  2-3 mm, bulk
  density 350 kg/m3
                      70 cm of activated, carbon, 1.5-2 mm,
                      bulk density 700 kg/m3
                      70 cm of gravel, 0.7 - 1.2 mm, bulk
                      density 1600 ,kg/m
Filter velocity
  25 m/h max.
Running times
:  about 7 days
Throughput/running
time
Manganese content
pH~ (corrected by
additions of NaOH)
  about 2500 m3/m2 :
  before the filter 2-6 mmole/m
  Mn = 0.1 - 0.3 mg/1 Mn

  after the filter 0.0 - 0.6 mmole/nf
  Mn = 0.00-0.03 mg/1 Mn


  ground water          6-9
  filter inflow         7.4-'
  waterworks outlet   '7.8

-------
                          - 535 -,
                                         Control
                                         !Chlorine :	^
                                       l  —T^pH  '	I
                               | •    Buflrtt jaravel    I I
                            	J L	J C	J   	,  , , ,
                            ~^i r-1i Y—-i i—« T~—i r—1 -  : r~. Chlorine
                            _J I I! _ I I U_j	U> -  ...-  -   to cons
  Fig.  3   Stiepel waterworks of -the WMR,  scheme  of the
          treatment, metering of potassium  permanganate
          into the draw-wells upstream of. the  filters
5.
Oxidation of manganese  in  oxygen-free water
In contrast to the  Ruhr  bank filtrate, the Rhine bank filtrate
coming from the gasworks and waterworks on the Lower Rhine  is
practically free  from  oxygen.   The result is that in spite  of
its relatively high content of neutral salts it is only
slightly corrosive.  This, situation would'be changed if the
freedom from oxygen were lost during- the treatment.  For  this
reason NGW did not  make  use of a-biological or autocatalytic
manganese oxidation in the process-technical design of the'
treatment plan for  waterworks 1,  and selected potassium per-
manganate as an oxidizing- agent.   The process scheme  (see
Fig. 4) has already, been described by Heymann  [6] at one  of
the 'earlier Karlsruhe  conferences.

-------
                          - 536 '«
      Ctuitic todn
 \
    \ T
j Preliminary filter * "&
0. 3 m Activated carbon, 3-5 sas 1
1.O m Anthracite, 1.6-2.5 inn
?. O.S m Filter asnd, o.5-t em J
f" O.2 » Tilter gravel, 1,5-2.5 las V
j
( Sflconaary filter

3


cti



container
^
.-44




^t
^•-T x-?-
?ere vator f j f

s -»
u
-.•i
/
5/w^

J_jv- - ._*^-,,, 	 1 i
            >   >     Ha

  I   — \   21  IMno. and *1 iol«li
     \\  !? I	•	1
s
1
I '
1 1
K «
n
1
^i »
Vv
v\
Braakoolnt cttlorin
Sodiun
hypochlorite
f — )j

1
t
n 	 v
1
.M-TOlUti
n
1
cp
          	€H  "-€!)—'
                                             i.-J
                                •^peculation ng*nt
  Fig. 4  Waterworks  1 of  the NGW,  scheme of the treatment,
          metering of pptassium permanganate into the draw
          flow upstream of the  filters
The potassitun permanganate  is  added in proportional
amounts to the raw water  in the .form of a 1% solution.  The  /•
                                                  3
specific amount of potassium permanganate (in g/m ) required
                                                2+
is 2.1 times the weight concentration of the Mn  , i.e. the
oxidizing agent is added  in a .slight excess over the sto.ichio-
                                    .»*
metric amount.  The  flocculating  action of the manganese
hydroxide formed on  organic water constituents is increased
                                                     •i
by the addition of aluminium chloride.  After a residence
time of 10 to 20 min the  flocks produced are removed,
following stabilization with a polymeric flocculation aid,
through a three-layer filter.   For the reduction of the
excess permanganate  ion the upper layer consists of activated
carbon.  This process removes  iron and manganese ions to below
the analytical detection  levels.

-------
                           - 537
Filtration of the water through a fibre-glass filter has been
shown to be a simple check on the potassium permanganate
dosage.  The amount added is- sufficient when a weak red
coloration is discernible at a layer thickness of about 5 cm.
Fully automatic regulation of the potassium permanganate
                                               •
addition is possible by continual measurements of the excess
KMnO,, but this is not necessary with the small changes in
the concentrations of iron and manganese in the Rhine bank
filtrate.

6.       Further experiments on manganese oxidation
The Witten waterworks of Gelsenwasser AG are situated in the
Ruhr valley about 8 km upstream of the Stiepel waterworks.
The so-called pure  water (up to 5000 m /h) obtained from
below ground consists of small proportions of bank filtrate
and true ground water, but mostly of artificially enriched
ground water.  It contains 1-2 mmole/m  = 0.05 - 0.10 mg/1
of Mn.  Because of the'various origins d~ the manganese, only
a partial load would be treated, by demanganization of the raw
water to be infiltrated.

Experimental treatments were performed following the prototype
method of the Stiepel waterworks.  These gave the surprising
result that potassium permanganate, in spite of the physical
proximity of the two waterworks and at first glance similar
raw water situation, did not here give the same good results.
Therefore, in the more recent large-scale plant the stronger
oxidizing agent ozone is used.  This example shows that
potassium permanganate, despite its advantages from the
economic and other points 'of view, is restricted in its uses
and the limitations must be"investigated in each individual
cape.                   '•*..'•

-------
                          -  538  -
7.       Disinfection of newly laid pipes with potassium
         permanganate
The disinfection of newly laid pipes is normally carried out
                                                      o
with chlorine water in a concentration of about 50 g/m  C1-.
In recent years various waterworks found that a single dis-
infection does not give the desired freedom from bacteria
even with 24-h duration of action.  This is particularly true
for pipes with a high wall alkalinity (concrete pipes, pipes
covered in cement mortar, pipes of asbestos cement, etc.),
presumably because of the reduction in the bactericidal
action of chlorine at higher pH.

Repeated high chlorination is a possible means — though
troublesome — of avoiding this disadvantage.  The DVGW
pamphlet W 291 "Disinfection of Water Supply Plants"  [7], in
the light of the. good experience particularly at the Hamburg
waterworks [8], which were the first to practise this process
on a large scale, contains the proposal to use potassium
                                             3
permanganate in a concentration of 5 - 10 g/m .  At Gelsen-
wasser AG sodium hypochlorite and potassium permanganate have
been used together for years to disinfect newly laid pipes.
The disinfectant solution normally contains 45 g/m  of C^
and 12 g/m3 of KMn04.

Fig. 5 shows the dependence of the redox potential of various
disinfectant solutions on the pH.  Similar results were
obtained earlier by Gras and Konrad [9].  If we start from
the assumption that the redox potential is a measure of the
bactericidal action of disinfectants not only at various con-
centrations of a particular disinfectant but also in a com-
parison of different disinfectants, the greater effectiveness
of pure potassium permanganate in comparison with chlorine
is not quite comprehensible, but that of the disinfectant
mixture is easily interpreted.

-------
                            539
400
                                         Fig. 5
                                         Redox potential of
                                         various solutions of
                                         permanganate and/or
                                         chlorine, measured
                                         against an SCE in
                                         dependence on the pH
                               PH
Under working conditions, the disinfectant mixture seems to
lead to the desired freedom from bacteria more' rapidly than
pure chlorine.  The only disadvantage is the greater expend-
iture- in the preparation of the solution, but' against this
is the advantage of the coloration of the .disinfectant sol-
ution in addition to the enhanced effectiveness already
mentioned.  During the addition flushing can take place at
the hydrant until the red-violet solution appears, and with
more recent rinsing analytical aids can be omitted.  A visual
check on disappearance of the disinfectant is sufficient.

-------
                           -  540 - • • .  .

Obviously  it must first be  checked whether a canalization or
drainage is available, into which the solution of disinfectant
containing the potassium permanganate can be harmlessly
introduced.

8.       Transportation and metering of potassium permanganate
Potassium  permanganate is usually supplied in the solid form
as a granulate in 50 kg drums.   At the place of its use
either a solution of constant  concentration is prepared and
metered out in liquid form  (see,  for example, Fig. 6)  or a
dry dosage is selected, regulated directly in accordance with
the fluctuating volume flow  of  the water.  All processes can
be automated, and it is advantageous if the potassium  per-
manganate  granulate is "free running".  A small addition of
silicate base (according to the  manufacturer's information)
clearly reduces caking of the  material, even at an elevated
relative humidity.

When the potassium permanganate  is used in larger quantities
the necessary opening, emptying,  and subsequent removal of
the sheet-metal drums is a  certain inconvenience.  On  the
suggestion  from Gelsenwasser one  of the larger potassium
                  Free-running KHnO4
                                to injection point
                    7
\T
                     Charging hopper
 Dry metering unit   \^  Moving membrane          !      !
          jVvl    ' '*!   i- Metering coil         i      !
          :- ~ '"z* ', '	~ ^*s.f                  ^|LN-    f'i^-
            --^J.'..'.-£-£'.^-> ' ".\ ,-l Level controller  >^k'    '^L,' Metering raump
                                              *
             /          '           \
    Dissolution chamber  Residence chamber  Supply chamber
 Fig.  6   Plant for the treatment and metering of potassium
          permanganate solution of constant concentration,
          schematic; after  (2)

-------
                           - 541 -
 permanganate suppliers obtained in 1975  special  permission
 from the  Federal Transport Minister to transport the potassium
 permanganate on roads in stackable cubic containers  (tank
 containers)  of up to 1050-litre capacity.  At  the same time
 a  filling installation for the tank containers was built at
 f   . •
 the  manufacturers.   In parallel with this, five  metering
 stations  of  the.kind shown in Fig. 7 were constructed at
 Gelsenwasser.   By using refined alloy steel tank containers
 -  shown  in  diagrammatic form  -  exchanged back  and  forth
 between the  suppliers and the consumer, the use  of potassium
 permanganate available in the solid form is substantially
 simplified.
                      Y-
 r"-- ''—"—..'^Dissolu-
 i |" i"i '" \ 'jtion
-| j.i;J. [; '•> |water f
                                     Seenaae reservoirs
 Rpsprvnir inflow
                               ^%^i   i    i   i
                                          \
  Fig.  7  Potassium permanganate metering  station with
          delivery in containers, schematic
9.       Summary
The present report  of  experience concerns the use of potassium
permanganate in the Gelsenwasser group.  While potassium per-
manganate is being  replaced more and more in the laboratory

-------
                          - 542 -
 as an analytical aid for the determination of oxidizability
 by stronger oxidizing agents,  the technical product is used
 increasingly in water treatment.   Potassium permanganate
 exhibits some characteristics,  although sometimes in a weaker
 form, of properties  and possibilities of use typical of the
 usual oxidizing, disinfectant,  algicidal,  and flocculating
 agents.

 -         As an oxidizing agent,  its  action on organic
          substances  is weaker  -  but by the same token more
          sparing —  than that  of  ozone and,  unlike chlorine,
          it does not form reaction products that remain in
          the water.

 -         As a disinfectant it  is  suitable for the dis-
          infection of plants but  not for disinfecting drinking
          water at the end of the  treatment.

 -         As an algicide it is  effective within the described
          limits and  has the particular advantage over other
          algicides of being permissible for the drinking
          water treatment of raw water.

 -         It may not  be regarded as a flocculation agent in
          the true sense of the term, although small floes
          with adsorbent and coagulating action are formed.

 -         Like some true flocculating agents, potassium
          permanganate is delivered in the solid form as a
          granulate and so requires a separate dissolution
          step.  As with other  flocculation agents,, the
          floes then  formed must be removed in yet another
          process step.

Potassium permanganate is a chemical  additive for water
treatment processes  which is used not in terms of individual
strong actions but rather as a combination of several weaker
actions.

-------
                          - 543  -
(]}  REIDIES,  A.H.,  MACK,  E.
    Die yerwendung  von Kaliumperxnanganat in der Wasser-
    aufbereitung in den USA
    Vom Wasser 3O (1963), 81-1O3

(2).MACK,  E.
    Die Verwendung  von Kaliumpermanganat in der
    Trinkwasseraufbereitung   .   "           •-
    Informationssschrift der Pirma Th.  Goldschmidt AG
    6/73 (1973), 21, 2-23,  siehe dort weitere Literatur

(3)  DIN 19619 Kaliumpermanganat zur Wasseraufbereitung
    Berlin, Beuth Verlag (1975)

(4)  SCHMIDT,  W.-D.
    Probleme  und Praxis bei der Algenbekampfung in
    Infiltrationsbecken
    Wasserfaehl. Aussprachetagung 1972  Dortmund
    Tagungsheft Frankfurt,  ZfGW-Verlag   (1972), 28-35

(5)  PlTSCH, B.
    MaBnalimen zur Verhinderung tibermaBigen Algenwachsturns
    in den Wassergewinnungsanlagen der  Gelsenwasser AG
    Bericht "Ruhrwassergiite 1976" des Ruhrverbandes/
    Essen  (1977)

(6)  HEYMANN,  E.
    Erfahrungen bei der.Anwendung eirier Flockungsfiltration
    vor den Aktivkohlefiltern
    Veroffentl.  des Bereichs und des Lehrstuhls ftir Wasser-
    chemie, Engler-Bunte-Institut der Universitat Karlsruhe
    (1975), 9, 107-118

(7)  DVGW-Arbeitsblatt W 291  Desinfektion von Wasserver-
    sorgungsanlagen, Frankfurt,  ZfGW-Verlag (1973)

(8)  GOLDA, W.
    Desinfektion von Rohrleitungen und  Trinkwasser-
    aufbereitungsanlagen mit Kaliumpermanganat
    Informationsschrift der Firraa Th. Goldschmidt AG
    6/73 (1973), 27, 34-35

(9)  GRAS,  KONRAD
    Klarung der Oxidationskraft des Kaliumpermanganats
    in Abhangigkeit vom pH-Wert
    Unverdffentl. Laborbericht 1972

-------
                          - .544 -

THE USE OF HYDROGEN PEROXIDE^IN- WATER,-TREATMENT  ,
H. Overath


1.    Introduction
In many respects hydrogen peroxide invites application as a
disinfectant and oxidizing agent in water technology:

-     Hydrogen peroxide can be easily and exactly metered
      out in the liquid state.

-     Hydrogen peroxide solutions between 35 - 50% are com-
      pletely safe when handled correctly.

-     Excess hydrogen peroxide can be removed quantitatively
      by activated carbon or by chlorine.

It is thus not surprising that hydrogen peroxide is from time
to time considered as an alternative to chlorine and ozone.
The following brief report attempts to clarify why hydrogen
peroxide has not yet established itself, and what are the
chances of its use in the future.

2.    The most important chemical properties of hydrogen
      peroxide

Hydrogen peroxide has three important chemical properties:

a)    It is an, oxidizing agent

      H_00 + 2H+ + 2e~ 	> 2 HO       E  = + 1.77 V
       £ £»                    £         O
      H,00 + 2e~	> 2 OH~            E  = + O.88 V
       £• £,                              (-)
b)    It is a reducing agent

      H9O_ 	> 2 H* + O- + 2e~         E  = + O.68 V
       t- £.             £•                O

-------
                          -. 545 -

c)    It can decompose"Into oxygen'and water in. an inter-
      molecular redox reaction

      2 H202	» 2 H20 + O2 +/46.9 kcal

3.    Examples of the application of hydrogen peroxide in  ,
the used water and waste water sectors,

For some time now hydrogen peroxide has been used for the
treatment of industrial waste waters whose components are
toxic to activated sludge and which can be readily oxidized
by it.  Thus cyanide is oxidized to cyanate, nitrite to
nitrate, formaldehyde to formic acid, and .phenol -  particu-
                           2+       3+
larly in the 'presence of Fe   and Fe   ions  - to the less
toxic pyrocatechol, hydroquinone, and quinone (Table 1).
Since hydrogen peroxide can oxidize H-S to elementary sulphur
and thiols to dialkyldithiols, it has already been used for
odour and corrosion control in the industrial and communal
waste water sector (Table 1).

In highly charged waste waters hydrogen peroxide is able, as
an oxygen donor, to maintain O7 concentrations such as cannot
be achieved on the basis of the entry values for atmospheric
oxygen given by Pick's law.  The same applies to the nitrifi-
cation of discharges of two-stage clarification plants by
filtration  (Table 1).

While large doses of hydrogen peroxide can in many cases be
used in industrial waste water treatment, only concentrations
of 1C mg/1 at the most are justified in the treatment of
drinking water for hygienic and economic reasons.  Since at
this concentration it is second only to ozone in oxidizing
strength, and it is known that on catalytic decomposition
hydrogen peroxide can form very reactive intermediate products

-------
Table 1 •«  Use of "
                                      o^
                                              t*ie waste  water  sector
Function of the
                              Process
                                                                                      Reaction
    as an oxidizing agent
     as an oxygen source
             -•Detoxification e.g. of

                   .- cyanide

                 ".- nitrite
                   .- formaldehyde


                   .- phenol



             -.Corrosion and odour control

               .Oxidation of -SH

             --Inflated sludge control
             --Degradation of biologically oxidizable
               •material (BOD)

             -•Nitrification in sand filters
                                                                           CN
                                                                           HCHO
            CNO

            N03~
            HCOOH
    (Fe2*K
                                                                                             >H
                                                                           H2S ~
                                                                           RSH
           RSSR
                                                                            unknown
aerobic biological (not chemical)
oxidation

aerobic biological oxidation of NH_  i
by Nitrosomonas and Nitrobacter     "
                                                                                                                      I
                                                                                                                      Ul

-------
                          -  547  -'
of sufficient lifetime, decades of effort have been devoted
to finding a suitable catalyst. -The stimulus is always the
hope that one's own work will be rewarded with the Discovery
of >the "correct" catalyst.  The magical intermediate products
are called:                  - .                .          .    •

         OH radicals and singlet oxygen.

These can both be formed from hydrogen peroxide and  are
extremely "fierce".       •

4.       Formation of the OH radical from hydrogen peroxide ,

The decomposition of hydrogen peroxide is strongly ;exothermic,
with an enthalpy change of AH = -22.62 kcal/mole.  Water and,
oxygen are formed as the final products:

         (H2°2>diesolved F=*  {H2°2^ liq. + X/2  (O2^gas    j

It is assumed that the non-catalysed decomposition of hydrogen
peroxide always proceeds _via hydroxyl and perhydroxyl radicals
in a non-chain reaction:

(1)          H202 	» 20H-    ;. •          '                ,  <
(2)   OH-  + H2O2 	*'H2° + H0°*
(3a)  HOO- + HOO	* H2O2 + °2
(3b)  HOO- + OH-
The initial reaction  (1) is rate-determining.  Since the diss-
ociation energy of the oxygen bond D(HO - OH) is 48.5 kcal/mole,
it is not surprising that pure hydrogen peroxide decomposes at
an immeasurably rapid rate.'

-------
                          - 548 -
The .thermodynamic instability of the hydrogen peroxide in
relation to its decomposition products H2O and O~, however,is
manifested in the feet that its decomposition rate is acceler-
ated catalytically by small quantities of a very large number
of substances in the dissolved and solid state.  Practically
all types of dust and dirt, in particular certain heavy metal
ions occurring in several valence states, and further more or
less all vessel surfaces have this effect, various reaction
mechanisms having been established for the homogeneous and
heterogeneous catalysis.

If hydrogen peroxide is added to Rhine water, the natural
concentration of potential catalysts is not sufficient for a
rapid decomposition since, according to our measurements, the
half-life amounts to a few days.  An additional catalyst is
required.

                                                       2
4.1.     Production of OH radicals by catalysis with Fe
                            ... •,                         2+
In most cases the catalyst recognized as the best is the Fe
ion.  In combination with hydrogen peroxide, it is known as
Fenton's reagent.  The first hypothesis about the reaction
mechanism was put forward in 1934 by F. Haber and J.Weiss  [1]:

START     (a)  Fe2+ + E2°2	*F-e3+ +,OH~,+ OH*

Unfortunately, in addition 'to the desired reaction with
organic molecules, the OH radical formed can enter, some other
undesirable subsidiary and radical-capture reactions  (Fig. 1),

-------
                             549  -
   desired
  OXIDATION
  REACTIONS
                   Fe2*- OXIDATION
                       MOO- O2  FORMATION
                         (radical chain
                          mechanism)
               further RADICAL-
               CAPTURE REACTIONS
                           +
  Fig. 1  Reactions of Fe   -catalysed
1.
It can (again according to Haber and Weiss)  function
as a catalyst-independent chain carrier  leading  to
O- formation:
CHAIN
          (b)  OH
         (c)  HOO- +
                           H00
                               +  OH-
         The new OH radical is .formed  at  the  expense  of two
         hydrogen peroxide.molecules.  Although  the inter-
         mediate perhydroxy radical can also  react with organic
         molecules, it is essentially  more  critical in this
         reaction than the OH radical.
2.
                                          2+
The OH radical can in addition oxidize Fe    ions:
(d)   Fe
                2+
                     OH
  3+
Fe   4- OH

-------
                          -  55u -
         This route is not only; undesirable as a radical-
                                                2+
         capture reaction but it also removes Fe   ions
         which are required for the initial reaction  (a).
         Although, according to H.H. Baxendale  [2], the
           2+                        3+  •
         Fe   can be reformed' from Pe    ions
         (e)  Fe3+ + HOO-
         this reaction is undesirable since the OH radical
         formation in accordance with reaction  (c) is no
         longer possible.  Moreover, the rate of this
         reaction is substantially smaller at pH 7 than that
                                    3+
         of the hydrolysis of the .Fe   ions.

         Finally there is              •      '

3.       A number of other conceivable reactions, such as
         hydration,rcharge-transfer-complex formation, etc.
         that deactivate the OH radical and so remove'it from
         the desired oxidative reaction.

         In other words, the yields of OH radicals available
         for the oxidation are related to the hydrogen per-
         oxide used, i.e. the  [OH*]/[HQO0] ratio, considerably
        =                              £t £t
         smaller than unity.   '                 •      •

                   2+
Fig. 2 shows the Fe  -catalysed reaction of hydrogen peroxide
with ethanol, one of the closer-studied reactions with an
organic compound.  The H-abstraction by the, OH radical takes
place predominantly at the a-carbon.  The oxidation to acetal-
dehyde and, further to acetic acid takes place preferentially
in the absence of atmospheric oxygen.  If oxygen is present,
the re-formation of the alcohol shown below plays a major role
with the intermediate organic radical functioning as an
                                        2+   '  "   3"+
electron carrier in the conversion of Fe   into Fe

-------
                          -  551  -
               Fe*
                           CH3-CH=0
                                 OXIDATION
                 /    y-H20»HO.
                 L-Y
                    H202
CH3-CH2OH J^CH3-CHOH   ^ CH3-CHOH
                     H3CHOH  ^r13-UnUn
                             DIMERIZATION
           HO.
                      [CH3-CHOH]
                            CH3-CH2OH
                                RE-FORMATION
  Fig. 2  Reactions of the OH  radical  with ethanol
It is therefore understandable  that  concentrated and.acidified
mixtures of hydrogen peroxide and  FeSO'4  are used; successfully
for the quantitative ashing of  foodstuffs.   However, in the
elimination of bacteria from drinking  water the intensifi-
cation of the bactericidal action  of hydrogen peroxide by
                       2+ '      •                      '
catalytic amounts of Fe   ions  is  less spectacular.   Results
from the Water Research Centre  at  Medmenham,  England,  sub-
stantially confirmed by studies in our laboratories  in
Wiesbaden in collaboration with the  Degussa Company, show that
even 120 mg'of H^O,,/! in 300 min destroy only 99% of E.coli.
                        2+                                •     •
If in addition 2.8 mg Fe . /I and 0.05  mM of EDTA are added,
the contact time necessary for'this  is reduced to :110  min
[3,4].  The bactericidal action, on the mesophilic and  thermO-
philic bacteria in Rhine water  was even  worse (Fig.  3).       ;

The preliminary results.of our  experiments  on the use  of
hydrogen peroxide'as an oxidizing, agent  in  Rhine water are
equally sketchy.  Evaluation of the  gas  chromatogram with an
ECD shows only relatively slight changes in the fingerprint.

-------
                          - 552 -
    H202 (mg/l)
                 50  110
               300 500 1000     5000 10000
               CONTACT TIME  (min)
  Fig. 3  H2O2 demand for 99% bacterial  elimination  (E.coli)
Only a few compounds, probably  as  a  result of  hydroxylation,
had their boiling points raised.  .These  phenomena should be
investigated more fully in  the  future, extended  to IR-spectro-
metric analyses, and compared; .with the action  of ozone.
4.2.
Production of OH radicals by photolysis
The production of OH radicals by  photons  in accordance with
the reaction:
                          hv
                   H2°2
                      2 OH-
                              2+
has the advantage over the Pe  - -.generated  OH radical  synthesis
                    Pe
                      2+
                    OH- + OH  + Fe
                                  3+
that two OH radicals are formed per hydrogen  peroxide
molecule.  However the difficulty8lies  in the fact that,
because of the large bond-dissociation  energy of  the O-O  bond,
only the shortwave UV-C region with wavelengths of 200-280 nm
leads to usable radical yields.   Therefore, at least in the
raw water sector, all the absorption  and  control  losses

-------
                          - 553 -
                               -.  f ''

caused by pollution of the .light-admitting surfaces will
                           4   ~ *"         .                      ,
occur.  However, in flocculated and filtered water the         •
combined use of hydrogen peroxide with ultraviolet for the     :
oxidation of organic .constituents deserves more thorough
study, all the more so since completely new antimony-doped     ;
mercury lamps emit ten times"as much in the UV-C region as
conventional mercury lamps and thus guarantee a high radical
yield.

5.       The formation of singlet oxygen from hydrogen peroxide
In add'ition to the OH radical singlet oxygen is a second very
reactive particle that can be formed from hydrogen peroxide
in a stoichiometric reaction with hypochlorite:

         NaOCl

The singlet oxygen is richer in energy by 22 kcal/mole than
oxygen in its triplet ground state and its chemical behaviour
is basically like that of an electrophi"1 ic olefin.  It reacts

a)       as a highly reactive Diels-Alder component with
         conjugated diene systems,

b)       in a (2 + 2)  cycloaddition with electron-rich
         olefins,
         and
c)       in accordance with the general reaction type of the
         En reactions with dlef ins" having an allyl hydrogen.

From these reaction types it can be seen that singlet
oxygen  -  similarly to ozone  -  reacts essentially more
selectively than the OH radical  (Fig. 4).

-------
 1,
 2.
 3,
        HC*^~ C
        * \  \
       HC—C—S
         R
     ROV,H
       r   ir
                          - 554 .-
   R        R
  ^.6=0   wr^C=0
no      " w
HCX   * HCX
   C-R      C=0
   M        I
   I        R
   0
                         hv
              (2.2J-CVCIOADOIT10N
            CH3
            H
            A,
                   9H3
                         EN-REACTION
                CH3-6-OQH
                            CH
  Fig. 4  Reaction types  of  singlet oxygen ( O2)
6.
Summary and outlook
Although hydrogen  peroxide is highly unlikely to win
a place as a primary disinfection agent, it promises
in conjunction with  low concentrations of other
                                2+
additives, such  as NH2C1 .and Cu   ions, a good
bacteriostatic action .on water en route to the
consumer.

The oxidizing action exerted on organic water
                 2+
components by Fe  -catalysed hydrogen peroxide
cannot compete with  that of ozone.  Nevertheless,
there are hopes  for  its use, combined with UV, in
water free from  turbidity.   Results similar to those
in ozonization with  subsequent activated carbon
filters are conceivable in-this sector.

-------
                         - 555 -
         The replacement of  potassium permanganate by hydrogen
         peroxide  for -demanganization has  already been con-
         sidered in connection with improving  the operating
         time of the downstream filters.


         Hydrogen  peroxide has up  to now been  used in drinking
         water  technology but not  as a supplier  of oxygen.
         However,  in the artificial enrichment of ground
         water  it  could serve as a source  of oxygen and help
         to  prevent the adverse consequences of  oxygen Con-
         sumption  in the ground.


         In  England a case is reported where hydrogen peroxide
         in  backwashing water considerably prolonged the
         filter running times.
(3)  SABER,  F.,  WEISS,  J.
    Proc. Roy.  Soc.  A. 147 (1934), 332-351

(2)  BAXENDALE,  J.H.
    Advances in Catalysis' _4 (1952), 31

(3)  -
    An Investigation of Hydrogen Peroxide as a
    Disinfectant of  -Potable Waters
    Water Research Centre, Medmenham, England
    Den.  (1974)

(4)  OVERATH, H.                      .
    Versuche mit H^O,-,  und UV-Strahlen
    ESWE-Bericht Nr. F 7/78

-------
                          - 556 •-


 SOME ASPECTS OF  THE USE OF  CHLORINE OR CHLORINE  DTOXIDE
 IN WATER TREATMENT

 J.  Valenta
The drinking water  in the  Zurich water supply obtained from
Lake Zurich has been treated with chlorine  for over  25 years.
Chlorine is still used as  the active agent  in the preliminary
chlorination, above all to protect the raw  water plants against
algae and DPP  (Dreissena polymorpha Pallas)  larvae.

Since 1971 chlorine dioxide has been used successfully as a
mains-protecting agent.  In the Zurich water supply  system
seven such C102 plants are at present in operation.

Although the concentrations of trihalomethanes as a  possible
result of chlorinating drinking water should give no cause
for concern, and although  we still know relatively little
about the possible reaction products associated with the use
of chlorine dioxide, we are investigating the idea of replac-
ing chlorine in the preliminary chlorination by chlorine
dioxide.  In connection with this, well-substantiated infor-
mation on the effectiveness of chlorine dioxide on "Wander-
muschel"  larvae,  and on the corresponding optimal  dosage would
naturally be important.

At Hardhof ground waterworks the bank filtrate is also to be
treated with chlorine dioxide instead of chlorine before the
inflow into the enrichment reservoirs.  To  this end  the
algicidal action of chlorine dioxide is currently being
studied by the limnological division of the Zurich water
supply.

Two further brief remarks  may be made on the two oxidizing
agents:

-------
                          - 557 i- •  >'  •

 The  first  concerns  the 'so-called- "stabilized  -chlorine d-ioxide
 solutions."   The  subject  was  discussed  2 years  ago at a
 conference in Zurich, and not much  has  changed  in these 2
 years.   Such  products are still  offered to the  drinking
 water works under various tradenames  as a highly effective
 concentrate of chlorine dioxide  which can be  stored for at
 least one  year without losing its effectiveness.

 On the basis  of several analyses  in a number  of  Swiss  and
 German laboratories, it can be stated that  this  product  is
 a sodium chlorite solution, which must  be regarded  as  im-
 permissible for the treatment  of  drinking water.   In  order
 to determine  the  C102 concentration,  the suppliers  prescribe
 only the usual iodometric analysis.   In this  method,  the
 chlorite ion  present is acidified and converted  into  chlorine
 dioxide, and  then determined  as  such.

 Finally, a remark on the  analysis of  the two  oxidizing agents:

 In spite of the ever-increasing number.of instruments  for
checking the residual amounts of.disinfectant by colorimetric,
amperometric,  or UV-photometric measurements,  in many  places
 simple manual methods are used for the  same purpose with the
aid of various comparators or simple portable colorimeters.
For the determination of  chlorine dioxide the colour disc or
the chlorine scale is used practically without -exception, and
the value found is converted to C1O2 with the aid of a factor.
The problem now lies in the fact  that, depending on which
 instructions are followed, this factor "=  2 is sometimes used
for multiplication and sometimes  for division.

Our own measurements have confirmed the practically only 50%
colouring action of chlorine dioxide in comparison with
chlorine both with OTO and with DPD.  We  found the mean value
for this factor to be 1.8, and the -chlorine value must be
multiplied by  it.

-------
                           -  558'-:

Consider three figures on  this  subject:               '•'"  ••'••-
Fig. 1 shows the simple absorption  curves  with OTO and DPD,
with a maximum at 436 nm for  OTO  and  two typical maxima at
511 nm and around 553 nm  (about 7%  higher)  for DPD.   In
addition to this the figure shows the exactly defined maxima
of the holmiura calibration filter.

Fig. 2 shows the peak for  chlorine, about  twice as high as
that for chlorine dioxide  at  the  same concentrations.

The last figure shows the  colour  development curves for three "
commercial reagents between the first and  the 26th minute.
This slide also reveals the high  instability of the colour
with one of the two DPD reagents.

Such facts, perhaps banal  at  first  sight can, however,  have
unpleasant and unexpected  results when put into practice in
various situations.
 1
 too ~
 9O-
 7r
  i

„„
Tl
431
t
jL
1

T)
»rms


« — Ho
._..




446nir
, 	 ,



. Uft 4f

._






















1 453.4nm i  - - —  ,   j
   ^—~1	553nm	-f-
    Stinm  jr,   =   ;
         r\     i
                            600
                                  Fig.  1
                                  Absorption curves  of chlorine
                                  dioxide  with  OTO (	)  and
                                  DPD  (-•-•-) and  of the  holmium
                                  calibration filter

-------
                           - 559-- -
                          d»2cm -I  \0.49mgC!/l
   500 nm     550     600   "  400 nm   450
                                         \

Fig. 2   Absorption curves of chlorine and chlorine dioxide
         in  equal concentrations with OTO and DPD
                  Colour development
                   CI02 -mit: DPD
                         DPD «B»

                         OTO
—
_.
—

••-
"•-




.— — — •
_.
J_ 1
j '
-.Lj-4-
_j 	 g_
4 / ' _
it;: .

44 - --:
.....
._.
-

i"
...

....
._



—




..:..




I
"• -1
_.
...


I J i 4 4 J
"1 LU...L
1 1 1 i !
i 1 | ill
1 ; : • ! •
• 26% '


i
•
>—
,-
I 1 . I J

-1 	 J
i


	
	 i 	 1
_1
—

! j«T,8%
	
.._


._l-.'
1
-O'TC

_
....
— i
.3% • 	 — 	 	 ;- 	 ^«0.6% _l_^:?A»_
	 ' ' ; , ill L...
-10.4% ", 	 i ~ • *';—• i~*—
i ! ! i ! ! I !
tf
~JL..
.^_:
'±:
j
3 5 10 15 2O 25
(VZunch. Au8«. 1978 Tittle, Klin
Fig. 3   Colour  development curves of  chlorine dioxide with

         various reagents

-------
                         -  560  -


BIOLOGICAL METHODS FOR THE TREATMENT OF GROUND'WATER
Y. Richard


I.     INTRODUCTION
In recent years renewed interest has focussed upon ground
water.  For example, in the United States the production of
water of subterranean origin has doubled in 20 years.

These waters are sought after for their organoleptic qual-
ities and the theoretical absence of organic pollution.
Nevertheless, their use sometimes entails considerable diffi-
culties: bacterial proliferation in the distribution system,
precipitation of iron or manganese compounds, and sulphurous
or unpleasant odour  -  and these are only the most direct
manifestations.

The cause of these various disadvantages must be sought in
the very nature of ground waters, wh_ich are characterized by
the absence of oxygen and which constitute a reducing medium.
In waters of this kind one may find, depending on the compo-
sition of the surrounding soil, the various mineral species
in their reduced form: divalent iron, ammonia, manganous
ions, and hydrogen sulphide.  All these elements are involved
in well known biological cycles.

Ground water contains no organic carbon and does not permit
the development of heterotrophic bacteria.  In the reducing
medium of ground water, in contrast, one finds a very small
number of specific bacteria in a state of latent development.
These are generally autotrophic with iron, manganese, or
ammonia.  It suffices for' the medium to be very slightly
aerated and set in motion, to create conditions favouring
bacterial development and leading to a profound change in the
quality of the water.

-------
                         -  561  - - •

Biological treatment is nothing more than the understanding
and control of this natural biological process, and the
development of plant required to accelerate it and to deliver
a water that will preserve .all its organoleptic qualities.

Apart from the diversity of the mineral species involved
(Fe, Mn, NHO , we must also define the principal character-
istics of biological treatment, while indicating the advan-
tages and the disadvantages of such treatments.

It should be pointed out that a different form of pollution
in ground water has been developing for some years.  The form
in question is nitrate pollution.  This ion can be eliminated
by biological means, using heterotrophic bacteria.  However,
this technique is rather similar to a reduction of nitrate
nitrogen N03 to nitrogen N, and we shall not deal with this
technique.

II.    THE BIOLOGICAL PROCESS
In general, all biological treatments make use of autotrophic,
or sometimes facultatively heterotrophic bacteria that use
CO2 and, for example, ammonia for their cellular development.
These gain the required energy from redox reactions.

II. 1.  BIOLOGICAL DEFERRIZATION
This relies upon f errobacteria, which have been studied in
particular by Hasselbarth and Liidemann (1) .   Many stations
in Germany make use of the principle of biological deferr-
ization.

In the list of bacteria drawn up by Starkay (2) (see Table 1)
we find the following species: Leptothrix, Sphaerotilus ,
Crenothrix, and Galionella.  When these bacteria undergo an
autotrophic metabolism, they draw their energy from the exo-
thermic reaction:
        4Fe(HC03)2 + 02 + H20 - > 4Fe(OH)3

-------
                                      -  562  -
TABLE  1    Various  types  of ferrobacteria        ,..,..-.--,•.>_
              (after  Starkay,  1945,  in  the  classification
               of  Prevot,   1961}
  Position in the classification
       Species
                                                                                 Remarks
 1. Sub-branch : Eubaeteria
   1.1. Class : Asporulales
     1.1.1. Order : Bacteriales
         1.1.1,1. Paiily : Protobaeteriaoeae
              Genus :  fhiotaoillus

 *l. Sub-branch ; Algolj&cteria
   f.l. Class : SiderobEcterialea
     2.1.1. Order s Chlamydobactiriales
         ?.1.1.1. Faaily : Chlanydobacteriaceae
              Genus :  Sphaerotilus
                      Leptothrix

         2.1.1.2. Faaily : Crenothrieaeeae
              Genus : Crenothrix
                      Clonothrix

         £.1.1.3. Faaily : Siderooapsaceae
              Geaur : Siderocapsa

                      Ferrobacillus
                      Sideremonas
     2.1,2. Order : Caulobacteriales
         2.1.2.1. Faaily : Galionallaceae
              GBDUE : Galionella
Th. ferro-oxydans
S. natans
S. dichotoma
S. diooophorus
L. ochracea
L. cr&ssa

Or. polyspora
Cl. fsrruginea
01. fusca

S. tretiMi
S. major
F. ferro-oxydans
S. eottferwnm
G. ferrtiginea
G. aajor
                    filanentary, coated

                    heterotrophio (problematic
                       antotrophs)
                      (or Cladothrix diohotona)
                      (or Leptothrix discophora)
                    nedia poor in organic matter
                    polluted media : heterotrophic

                    facultative autotrophic
                    heterotrophic
                    (problematic autotrophs)
                    pedicular

                    strictly autotrophic
                    nedia poor in organic matter

-------
                         - 563 -
II.2.  DEMANGANIZATION
Organisms that oxidize manganese have been studied by
Schweisfurth   (3) and by Mulder and Van Veen  (4) . "They are
listed in Table 2.
 II.3.  BIOLOGICAL NITRIFICATION
 Nitrification reactions  that oxidize ammonia  to  nitrate  rely
 upon autotrophic bacteria.  The  nitrification process  is
 divided  into two stages, which bring into  play two  types of
 bacterial  strains.   This balance is described empirically by
 the following equations:

       Nitrite  formation
 During this phase the  ammonia is oxidized  to  nitrite:

 55  NH4+  +  76 02 + 109  HCO3~  Nitrosomona»
                             C5H?NO2 +  54  NO2 + 57 HjO  + 1O4

       Nitrate  formation
 The phase  in which  nitrites are  oxidized to nitrates:

 4 N02~ + NH4+  + 4  H2C03  +  HCO3~  """"        *
                                 C5H7N02 * 3 H2O + 4OO NO3 + 195

where  C-H-NO-  represents  the  chemical  formula  of the  bacter-
ial  cell.

-------
                          564 •-••t
TABLE 2  Microorganisms capable of oxidizing manganese
         (after Mulder and Van Veen, 1963, and
          Schweisfurth, 1972)
 A. BACTERIA
    1.
    1.1.
    1.1.1.
Sub-branch  Eubacteria
Class : Asporulales
Order : Bacteriales
    1.1.1.1. Family : Pseudomonadaceae
          Genuses :  Pseudomonas  » :.
                     Metallogenium
                              Ps. manganoxydans
                               M. personatura
                               M. symbioticum
    2.
    2.1.
    2.1.1.   Order
    2.1.1.1. Family
          Genuses :
Sub-branch  Algobacteria
Class : Siderobacteriales
        Chlamydobacteriales
         Chlamydobacteriaceae
        Leptothrix :
                     Sphaerotilus
    2.2.     Class :
    2.2.1.   Order ;
    2.2.1.1. Family
          Genus i
                       L. echinata
                       L. lopholes
                       S. discophorus
               (or Leptothrix discophora)
Thiabacteriales
Hyphomicrobiales
 Hyphomicrobiaceae
Hyphomicrobium :       H. vulgare
    3.       Sub-branch  Mycobacteria
    3.1.     Class : Actinomyceta'les :  '•
                     Certain unidentified Actinomycetes,
 B. PROTOZOA
    Flagellae branch
             Family :  Monadaceae
                           •  Anthophysa vegetans
                        (ace.. , to Pringsheim, 1966)
 C.  ALGAE
  '  An alga of the Diatomea class    (ace. to Peklo, 1909)

-------
                           - 565.-
  TABLE._3   Microorganisms  capable of oxidizing ammonia
 Microorganisms   responsible  for  nitritation:
 -    Nitrosomonas  europea  and monocella
 -    Nitrococcus    -
 -    Nitrosospira              ,  .
 -    Nitroscystro

 N.B. Nitrosomonas  is the most abundant,  and  its  activity
      the.greatest.
 Microorganisms responsible  for nitratation:
 -    Nitrobacter winograd_skyi
 -    Nitrobacter agile-
 -    Nitrocystro
 -    Bactodenna
 -    Microdenna
III.   DEVELOPMENT CONDITIONS
Bacteria will only survive in a medium that conforms to
certain conditions of pH, salinity, redox potential.  The
redox potential ranges vary appreciably according to the
bacteria.   .           ,.:••-,.

All the bacteria that affect the oxidation phenomena of
ground water (iron, manganese, ammonia) develop in aerobic
media and require the water to be-aerated.  Pig. 1 shows that
the introduction of very-small quantities of oxygen brings
about a rapid increase in the redox potential.  The intro-
duction of 0.2 mg.Jl"1 produces a change of more than 250 mV
in the water.  However,'variations in E^ are not always
favourable to the development of all bacteria.

-------
                          - 566 '-..I
   KV/H- ELECTRODE
  300
  280
  260
  240
    /
/°                                Redox potential
                      DISSOLVES OXYGEN (MG/O
    0    05   1    1.1   2    2,5
For each type of bacteria we shall state the optimum redox
potential conditions  that favour their development.

III.l. DEFERRIZ&TIQN
Biological deferrization  by ferfobactefia has been studied
in particular by Hasselbarth and Ludemann, 1971 and 1973,
who quote very high passage velocities, and retention capacities
for filters working on  this principle; many plants of this
type are now in existence.   _••;•:

The pilot plant upon  which we conducted tests is described in
Pig. 2.  The aeration system is designed to intrcduce only a
limited amount of oxygen  into the water (this being one of
the main differences  compared to the physico-chemical process).
The results were as follows:

-      Biological deferrization developed under the following
       conditions:

-------
                   - -  5,67 .<--.'.
                                     Fig. 2        '• ..;.
                                     Biological  pilot  plant
   ANTHRACITE
   1,5-2,4 HM
SAND
1,0m
   dissolved oxygen  concentration: 0.2 to 2 g-m
   pH:  identical  to that of the untreated water
   (6.3 - 6.4)
   redox potential:   40  to- 200 mV on average
   rH: . 14 to 20;  if rH  < 14,, some Fe' is left in
 ,  the water.. 'For a pH  of 6.3 - 6.4 and a potential
   of 100. to 200 mV,  biological def err ization takes
   place at the boundary between the..Fe   and _Fe (OH) ->
   zones  (see Fig. 3).   Any excess oxygen will result
   in an ,i,ncr,ease  of "the redox potential.    =
   If, on the. other  hand, xH > 20 the filters axe
   likely-to become "rapidly clogged'since physico-
   chemical1 deferrization1 (with precipitation of
   amorphous floccular material) then competes with
   the activity, of the ferrobacteria.

There was no formation of a silica-iron .complex. .

-------
                         - 568 -
                                        Fig. 3
                                        Potential pH diagram
                                        for iron
      1  -2 3  4  S S 7  8  9  10 11 1!  13-14pH
III. 2. NITRIFICATION
The curves of the redox potential as a function of the pH
(see Fig. 4) , show that nitrification takes place in poten-
tial regions higher than deferrization, between 300 and
500 mV.  Nitrification is manifested in a fairly wide range
of pH between 5 and 10, but the optimum pH range is about 8.
The nitrification yields fall rapidly below pH 7.
Nitrification requires more oxygen "than deferrization.  4.53 mg
of oxygen are needed to transform 1 mg of annnoniacal nitrogen
into nitrogen in the form of nitrate.

-------
                         - 569 *-,_:•
                                   Fig.  4
                                   Potential pH diagram for
                                   nitrification
III.3. DEMANGANIZATION
The results for manganese are less clear.  Manganese can only
precipitate in an alkaline medium"  (pH >_ 9).  Nevertheless,
the redox diagram does not exclude chemical precipitation of
manganese between pH 8.0 - 7,5 and at a redox potential
between +400 and +500 mv".  However, the reaction should be
very slow.                          '         .

The manganese bacteria can develop above pH 5.5 and the ground
water must be aerated so as to give a redox potential between
+100 and +200 mV if any biological oxidation of manganese is
to be achieved.

III. 4. SIMULTANEOUS ELIMINATION OF SEVERAL ELEMENTS
Manganese, iron, and ammonia,are often found together in
ground water at various concentrations! the concentration of
the manganese is often the lowest.  Are there specific  con-
ditions of redox potential and pH which permit elimination

-------
                         -  570 -

of all three elements, and  if- so, •-under--'what conditions?
Several situations may be encountered.

a)     Large amount of iron in the presence of ammonia and
       manganese
This case occurs frequently in ground waters.  The intro-
duction of very small amounts of oxygen, monitored -by
measuring the redox potential, enables biological elimination
of the iron by filtration.  The quantity of oxygen is-always
sufficient to eliminate traces of ammonia of the order of
0.2 to 0.5 mg-2,   NH^  either by nitrification or by bacterial
assimilation.

Hasselbarth has observed- bacterial elimination of manganese
at the deferrization filter.  This would be made - possible by
a very small addition of oxygen, to give the correct redox
potential.  Nevertheless, true manganese bacteria are not
found in the filter (5).   •   .    ,  -

In many cases iron is eliminated chemically in a first filter,
and the biological elimination of manganese begins at the
bottom of this filter and continues in a second filter (see
Table 4).

b)     Large amount of ammonia in the presence of iron and
       manganese
If the quantity of ammonia pre.sent in the untreated water is
greater than 2.5 mg NHL per litre, the ammonia can be elimin-
ated by means of an immersed filter_ packed with pozzolana,
into which air is blown to satisfy the oxygen demand required
for nitrification.  The most favourable air/water ratios lie
between 0.6 - 1.2.  The ammonia is eliminated at the pozzolana
filter.  The redox potential of ,the. "water increases consider-
ably with aeration, and deferrization is effected by chemical
means.

-------
                          - 571 -
 TABLE 4  Deferrization in the presence of manganese
          and ammonia

Raw water
Water F
Water F
Rate
m/h

10
10
Iron
mg/1
5-15
0.1-1
0.01
Manganese
mg/1
0.3 - 2
0.1 - 0.5
O.O1
mg/1
0.1 - 0.3
0.05
O.05
 - Aeration by spraying
 - Reaction tank: 1 h
   Filter  I: sand depth 2 m
   Filter II: sand depth 1.5 m
   Duration of filtration cycles: Fn
                                   II
                               48 h
                                1 week
The precipitation of ferric hydroxide leads to a reduction  in
the number of filtration cycles and entails frequent cleaning
of the sand filter placed behind the pozzolana filter.
IV.
KINETICS OF TH£ BIOLOGICAL PROCESSES
When the pH and redox potential conditions become favourable,
the biological process is observed to develop very rapidly
after an incubation phase that may be fairly long (from 3
days to 1 month).

-------
                          - 572
The growth of the bacteria -can be>• expressed by the'following"
relationship:
                i  =  „-.

in which x = concentration of microorganisms (mg-.£  ),
         y = specific reproduction rate of the bacteria
             (day"1)
         cr = mortality rate of the bacteria (day  ) .

The specific reproduction rate of the bacteria depends on the
concentration of the substrate S to be eliminated, in accord-
ance with Monod's equation.  In our case iron, manganese, and
ammonia are designated by the letter S.
where u  = maximum specific  reproduction rate (day" ) ,
       S = concentration of the mineral substrate to be
                           ~. i
           eliminated (mg? £  ) ,
      K  = concentration of S when p = ~jp.
Moreover, the elimination of the substrate is proportional to
the concentration of bacteria present i\n the medium.  We have:


              ds -  Y ££                                 (3)
             ~ dt ~  * dt

where  Y is the cell yield.

By substituting eqs. 1 and 2 into 3, we obtain the substrate
elimination rate:
                 _
               dt ~     Y  (K  + S)     Y
                           S
                                   _  £   x                 (4)

-------
                          -  573  -
If we put  —— =  q  and  ?-  -=• K ,'an 4endogenesis term,
equation 4 becomes:
The  importance of equation 5 is related to the kinetics of
mineral substrate elimination, which govern the dimensioning
of the water-treatment plant to be brought into operation.

In effect, the reaction order may vary according to the con-
centration of S to be eliminated.  The*K„ values of all the
elements considered are small; for example, for ammonia K
                     -1                   '
is less than 0.1 mg-i   of ammoniacal nitrogen and the value
of K  may be,neglected in comparison with S.  We shall assume
that for the filtration rates used during the treatment of
surface waters the distribution of the biomass is homogeneous
in the filter.  If S »  K , -equation 5 becomes:
             ^         _
               ctt         (3,

The elimination of S is independent of the concentration of
S and the reaction order is zero with respect to S.

Integrating eq. 6 and putting t = yj, where U is the mean
filtration rate, we obtain
        So- S  =  (q - Kd)      X                         (7)
Thus the S-elimination profile is linear  (zero order)

If S <_ K , eq. 5 becomes:

         dS  _  ,*  S	K .   x
         dt     tq  K      d;

-------
 The term K,K  can be neglected, and integration gives:

                       - 3_  . H
            S  =  S e    K    U
                   o      s
                         
-------
                          - ^575 - '
where  K

       H
       U
       8
                     - 10 °C)
depends on  (q - Kj)
filtration material,
                                                          (11)
                                X and on the size of the
           depth of the bed  (in metres) ,
           filtration rate  (mrh   ),
           a value between 1  and  1.3.
This equation expresses  the maximum quantity AS of substrate
that can be eliminated by a filter of depth H,  packed with
a given filtration material and  operating at a given rate.
The ratio H/U is the apparent  residence time of the water.
Since the temperature of well  waters is constant, and close
to 12° Cr it does not constitute  a determinant factor.
In this case the elimination  profiles  of both ammonia and
iron are linear  (see Figs.  5  and 6).
                 WATIR THROUSHPWT » 4173/1717
                                                 0, DISSOLVED H6/L
                                                  2    i
                                                     DEFTHCn)
 Fig. 5  Ammonia  elimination profile

-------
                           — 57'6 J-
            FILTER CHARACTERISTICS!

            - ANTHRACITE 1.6-2,3 MM

            - BED HEI6HT! 1.25 H

            - FH.TRATIOH RATE! 10 H/M

            -rH 6.3
            - DISSOLVED OXYGEN! 1,8 PPM
                                Fig. 6
                                Biological  iron elimination
                                profile
                      FILT1H BEPTH {«)
         0.5
            IS
V.I.
INFLUENCE  OP THE FILTRATION RATE
Pig. 7  shows the influence of the water  throughput on  the
quantity of ammonia eliminated by a filter 4 m in depth.  The
quantity eliminated decreases with increasing throughput.
While one can eliminate  12  mg.£   of NH.  at 4 m«h   , the
quantity of ammonia that can be eliminated falls to 3 mg«-£
to 20 m-h  .
                                                      -1
V.2.
DISTRIBUTION OF THE BIOMASS
Equation  11 also shows  that if one wants  to eliminate  large
quantities of substrate,the residence  time must be increased,
either by increasing the  depth of the  filter bed or by
lowering  the water throughput.

-------
                          --577J-
 15
 10
    ELIMINATED
            \
                                           BED DEPTH 4ft
  0
                                         WATCR THROUGHPUT H9/N2H
                        10
                               15
                                 20
25
 Fig.  7  Quantity of ammonia eliminated by  an  immersed filter,
         as a function of the water throughput
Experience shows that  it  is  not advisable to reduce the water
throughput too much, since the biomass will then be poorly
distributed.  ATP measurements along the filter show that
its distribution along the filter follows the equation
       -KH
X
X e
 o
(see Fig.  8), which results in a non-linear
profile.
In this case,  to  eliminate the same quantity of the substrate
AS, one must achieve a longer residence time and this again
entails an  increase in the bed depth, in contradiction  to
the objective  sought initially.

-------
                         - 578 -
Log(ATPou?1H*4)
 101
  5
                               D
 0    25
. ATP  Pg/ml/cm
aHH4  mg/l
                    87
140
                         8° C
                         I mVm2.h
                                        Fig. 8
                                        ATP and ammonia profiles
                                        along a submerged bed
V.3.
     INFLUENCE OF THE LOADING  S.
Equation 11 also shows that a biological  filter eliminates
a given quantity AS,  and that if  the  loading S at the input
to the filter is increased, the filter will still not elim-
inate any more than the amount AS in  equation 11.  Fig. 9
shows that when the NH, loading at the input increases from
               —I
2.5 to 6.5 mg» fc  ,  the filter continues to eliminate.a
constant amount of  NH. (3 mg-2. ) ,  so that the amount of NH4
                                                  _i
present in the effluent is increased  (0 to 3.5 mg*&  ).
One must therefore take into account  the  nature of the bore-
holes and the future course of pollution  when calculating
the depth of the filters,   A safety margin must be allowed
for in case the loading S  increases.

-------
                         - 579 -
                                               _._ NITR1CATOR INLET
  4 10
      AUGUST  .  OCTOBER
Fig. 9  Nitrification cycle
         (pilot trial pnder pressure?  bed packing depth 2 m)
The case when S is small:
Reaction 10 is applicable and  shows  that the elimination is
of first order.  This case  corresponds to the elimination of
trace amounts of NH.,

It should be noted that, all other  things being equal, a
filter 4 m in depth will enable  the  ammonia content to change
from 15 to 0.5 mg«£   i to change from 0.5 to 0.05 mg«£   one
would then have to add another 1.5 m of depth (giving a total
depth of 5.5 m) - see Fig.  5.
V.4.   INFLUENCE OF THE MATERIAL  SIZE
The size of the materials used  influences:
-      the biological growth
-      the filtration.

-------
                          - 580 ->u- -
a)
Biological growth'
                           trt'f -j T
The biomass forms  a  biological film which grows around  the

material and develops  an exchange area that will be the

greater, the greater is the specific surface area expressed
    2  -3
in m ».m  .  The  finer  the material,•.• the higher the activity.


Pig. 10 shows the  quantities of ammonia" eliminated by two
grades of pozzolana, one with a size between 3 and 5- mm and

the other between  5  and 10 mm-.   „.•.„•
                TEST TEMPERATURE: 11°C '
                     — POZZOUNA (^-£ MM)
              POZZOLANA (5-10 MM) —*
                                 m'
                                " T"— "
                                           Fig.  10

                                           Influence of material
                                           particle size on the
                                           quantity of ammonia
                                           eliminated in an
                                           immersed'filter '
 b)
 Filtration
 Can one, however, use  finer and finer materials to.  improve

 the efficiency  of the  biological treatment?
 In the case of  classical filtration proceeding from  the  top

 downwards, and  if  we accept the Kozeny-Carman model,  then

 for a filter of depth H packed with a material of  specific

-------
                          -  581  ^r  .


surface area  a  the specific pressure drop may be written

        AP    _, „.  2   (1  — e)
           . K v a   	3— .   .
                        £

where  n = viscosity of  the water,
       V - filtration rate,
       a = specific surfa.ce area of the material,
       e = porosity,
       H = depth of the  filter bed.

We see that an increase  in the specific surface area results
in a very rapid increase in the initial specific pressure
drop across the filter,  and leads either to a reduction in
the duration of the cycles, or to the use of more powerful
pumps i

This formula also shows  that the pressure drop further depends
on the porosity of the material, according to the factor:
(1 -e)2  ,                             ,
During the filtration cycle, the elimination of the Fe, Mn,
or ammoniacal N is accompanied,by a.development of the bio-
mass around the filtration material and the porosity
decreases with time,' causing, the tdtal pressure drop across
the filter to become greater, - Thus, the evolution of the
pressure drop and the frequency of cleaning out the filter
will be governed by the -grain size of the material and the
bacterial reproduction rate..

Influence of the reproduction rate on the frequency of cleaning
out:

-------
                         -  582  -
   ' ' -     • •  >     • -. , '» • . .  - ., ,  ) I . .  ] .. „ . , . .'-   ,   '  ,   ' . .  :'. '
The reproduction rate  y  can easily be related to the cell-
division time, also known as the doubling time  (tj):


          = 1  clX = d"Log S _ 'O,693
        v   X  dt      dt      ' t
In the case of a filter operating correctly, where the con-
centration of Fe, Mn, or ammoniacal nitrogen at the filter
inlet is constant and greater than K , the biomass grows
                                    o
exponentially according to:

                    X = Xo eyt
The exponential growth of the biomass within a filter rapidly
reduces the porosity and increases the pressure drop across
the filter.

The bacterial reproduction rate var-ies from one species to
another, and one finds different cleaning sequences in
operation, depending on whether deferrization or nitrification
is taking place.

-      Nitrification:        ......
Thus, according to the basic equations, the elimination of
20 mg. £   of ammoniacal N will produce 3 mg•&   of Nitro-
                         _ i
somonas (10) and 0.5 mg.&   of Nitrobacter. .The elimination
of 5 mg of ammoniacal nitrogen at 10 m»h   will produce
     _3
8 g-m   of sludge.
 -       Deferrization:
 Our  tests of  biological deferrization enabled us to eliminate
                                   2                     —1
 an average-of 4000  g  of iron per m  over 30  h at 10 m-h  ,
          _2
 or 133  g-m   «h of  iron.   This iron accumulates within the
 filter  in the form  of  a ferric sludge and the filter must  be
 cleaned on  average  every 30 h.

-------
                         -  583 -
A rapid calculation shows that the nitrification filter will
in theory attain the same max:m,  pernissible pressure drop
in 20 days.           .

In fact, in the Paris region we have been using immersed
filters for nitrification for 15 years, packed with pozzolana
in the size range of 7 to 15 mm.  The use of a coarser material
limits filter cleansing to once a month.  In the case-of
nitrification under pressure (1.2 bars), the filters- needed
no washing for a' whole year.

V.5.   INFLUENCE OF THE METHOD OF WASHING
For a biological filter to work well, the biomass must be in
its exponential growth phase.  In the event of major cleaning
the biological activity of the filter is reduced.

The order of the reaction, which is 0 or i for optimum oper-
ation during most of the cycle, becomes 1 at the start of the
cycle.

Excessive washing will remove a very large fraction of the
biomass fixed to the filtration material, leaving behind a
total initial biomass that is too small, and that must be
regenerated at the start of the cycle.              ;

In the case of biological deferrization, washing has a funda-
mental influence upon the filter-maturation time.

The best results have been obtained with:

       Blowing the sand with air only:  5 to 10 sec.

       This blowing allows .elimination-of old trichomes.

-------
                          -  584  - ••••
-      Rinsing with water only, 10'0'm-fr   - 3 min.

This rinsing causes a slight expansion of the material without
impoverishing the biomass within the filter.

VI.6.  CONCLUSION
An understanding of the biological' factors that govern the
biological processes makes it possible to eliminate undesir-
able elements such as iron, manganese, and ammonia by bio-
logical filtration.

The plant operation, packing depths, and the choice of the
particle size of the filtration 'materials depend closely
upon the element being removed and on the growth Qf the
bacteria.  These treatments can be relatively difficult to
use unless the pH and redox potential conditions required
of the bacteria are provided.  The loading variations from
different boreholes can give rise to poor elimination of the
undesirable element, and it is necessary to include an
aeration zone and a filtration zone.

-------
                         - 585
 (1) ESSSELBARTH, U. , ''LUDEMANN, -D.   ,   .        •    f
     Enteisenung und Entmanganung
     Vom Wasser 38-  (1971),  233-253

 (2) STARKAY, R.L.    ,   > •  •.  . .; ,  .
     Transformation of Iron  by Bacteria .
     J. AWWA 37 (1945) ,  963-984

 (3) SCHWEISFURTH, R.
     Manganoxydierende, Mikrporganismen in Trinkwasser-
     versorgungsanlagen
     gwf-Wasser/Abwasser '113 '(1972) ,  12 ,  562-572      :

 (4) MULDER, van VEEN
     Investigations on the Sphaerotilus Leptothrix'group'
     J. Ant van Leenwenhook  29 (1963), 121-153

 (5) H&SSELBARTH , U . ,' LfJDEMANN ,";D .    ; '
     Removal of. Iron and- ..Manganese  from Ground Waters by
     Micro-Organisms        '
     Water Treatment' arid'' Examination ;22_ (1973) , 1, 62-77

. (6) DEVTLLERS     ,   ....... \ ....
     Nitrification ;de' i''ea'u:  'e'limination de 1'ammoniaque
     des eaux d-'alimentation -.•; •    • ,:    . -     '     •  .  •
     T.S.M. L'EAU^Oct. 1965..

 {7} BRENER,t L.. , RICHARD^- ,Y..-, • .MARTIN ,•  G.. '   .      •
     Elimination de 1'aramoniague  des eaux  de surface -
     Conference prononcee au 58e "cbngres  de 1'A.G.H.T.M.
     Bordeaux (1978)

 (8) MOUCHET, P., MAGNIN;, J.
     Un cas complexe de deferrisation  d'une eau souterraine
     58e congres de 1'A.G.H.T.M., Bordeaux (1978)

 (9) RICHARD, Y., BRENER, L.,  MARTIN,  G.,  LEBLANC, C.
     Study of the nitrification of  surface water
     I.A.W.P.R. 9th International Conference

(10) EAUG, R., MCCARTY, P.L.
     J. WPCF 44 (1972),  11,  2O86

-------
                         - 586  -


BIOLOGICAL REMOVAL OF AMMONIA

J.B. Goodall


Introduction

Pilot scale studies to evaluate cost and efficiency of
ammonia removal from River Thames water by biological
sedimentation  (fluidised bed),  biological filtration,
and air stripping, were carried out between 1971.and
1975 at the Medmenham Laboratory of the Water Research
Centre (1).
The early work quickly revealed that biological sedi-
mentation was both technically and economically the best
of the three processes and so the work was extended
with the specific aim of being able to advise on the
design of full scale plant (2).
This paper describes the biological sedimentation pro-
cess and gives brief comparative details of the other
two processes.
All the work was financed"-by-the Directorate General,
Water Engineering, of the Department of the Environ-
ment.

The ammonia problem     *

When ammonia is found in surface waters it is always
as a result of pollution, usually from the excretions
of wild and farm animals and sometimes from untreated
or imperfectly treated sewage or leakage from sewers,
cesspits, and  septic tanks.
Ammonia is occasionally found in. groundwaters as a
result of biological denitrification or from the break-
down of protein by saprophytic bacteria and fungi.

-------
                           587 -
It is believed that ammonia in drinking water does not
present any direct health hazard and it is interesting
to note that from many samples of water from distribu-
tion mains analysed at Water Research Centre there has been
little or no evidence of oxidation of ammonia to nitrite
or nitrate. Work in the Netherlands has shown, however,
that levels of ammonia in excess of 0.3 mg/1 as N can
lead to aftergrowths in the distribution system and
associated taste problems. Ammonia is toxic to fish,and
ammonia removal may become an important aspect of fish
farming, particularly where water is recycled.
There is no doubt that ammonia interferes with chlorina-
tion although the hazards of poor disinfection have
been exaggerated in the past. The real disadvantage is
the cost of the large chlorine dose required to oxidise
ammonia and the difficulty of controlling the dose when
the ammonia concentration varies. Increased chlorine
dose can also presumably increase trihalomethane .forma-
tion.
World Health Organization  (WHO) European standards re-
commended a level of not more than 0.05 mg/litre in
supply.                                          .f

Non-biological ammonia removal processes

Air stripping                                  "   . •,
In this method the nitrogenous material is removed from
the water - not merely converted into a different chemi-
cal state. This advantage must be offset against the
possibility that ammonia gas discharged to atmosphere
may cause air pollution or even re-dissolve in open
water.

-------
                         - 588 -

As ammonia  is  extremely soluble a high air to water ra-
tio is required  for efficient stripping, pH is also im-
portant, high  values giving best results because only
gaseous ammonia  can be  removed by air stripping and it
is necessary to  hold the pH between 1O and 11 to get
9O% of dissolved ammonia in the gaseous form.
Experimental work
The experimental work was  carried out in two rectangu-
                                     2
lar mild steel towers each of Q.58 m  area and containing
2.1 m depth of 1O x  5O mm  serrated wooden slats (Fig. 1).
Water entered the towers through distributors at the
top and air was supplied from centrifugal fans at the
base.
   DETAIl OF PACKING CONSTRUCTION
                                         Fig. 1
                                         Air stripping tower
  PUN VIEW OF
  OtStWBWCft

-------
                         - 589--

The  best .result consistently obtained was 9O% removal
at pH  11 with a water to air ratio  in the range O.12
to O.16 by weight.
Operating problems
There are potential operating problems  in  the  air strip-
ping of ammonia.
1.   Ice formation  from evaporative cooling in  cold
    weather.
2.   Calcium  salt precipitation from CO2  stripping. •
3.   Temperature effect - efficiency reduces with fall-
    ing-water  temperature (Fig. 2) and  reduced air tempe-
    rature also affects the process adversely.
 100-1    	 "-I  ii;11  _
                       BIOLOGICAL
                       SEDIMENTATION
                       is unaffsctsd by
                       temperaturas of 5 ta 20 °C
 C
 n
       f
 H

 •H
                                         Fig. 2 ,
                                         Effects of
                                         water temperature
                     20

-------
                            590
 The cost of air stripping will always be  much higher
 than that of biological processes  (Fig. 3).  The major
 cost is running the  air blower but the cost  of pH ad-
 justment may vary widely depending on the buffering
 capacity of the raw  water. Air and water  temperature
 has a big effect on  cost - it has been claimed, for ex-
 ample, that both capital and operating costs of air
 stripping would be three times higher in  Sweden than
 in California  (3) .
   Cost
   BIOLOGICAL
   SEDIMENTATION
   is the chtapsstby far-
   even for ammonia
   concentrations as low as
   01 to 0-2mg«/l«tre
                   6-
                   4-
                   2-
                          A1R STRIPfMNG
Fig. 3
Comparative costs
                      AMMONIA CdNCElwnUTfm (hi,/I)
Breakpoint  chlorination

Ammonia  is  converted to chloramines.  Conversion to tri-
chloramine  requires a chlorine dose about 8 1/2 times
the ammonia concentration in addition to the dose re-
quired for  disinfection. The method has the disadvan-
tage of  increasing the chloride content of the treated

-------
                         - 591---'

water and control can be difficult if the ammonia con-  ' '
centrations vary widely. High chlorine dos'es could pre-
sumably increase trihalomethane formation.
Breakpoint chlorination is worth considering at very
low ammonia concentrations or following'biological
sedimentation when the dissolved oxygen content of
the raw water limits.biological removal.  (See later). •
Breakpoint chlorination can be used under-these circum-
stances to remove any small excess passing through the
biological sedimentation unit.

Electrochemical removal  (electro-oxidation)
%
It has been demonstrated that it is possible to convert
ammonia in sewage directly to nitrogen gas at the anode
of an electrolytic cellf but high efficiency is only
possible if very expensive platinum electrodes are used  (4)
Fouling and corrosion would probably have a considerable
adverse effect on cost.

Biological ammonia removal processes

Biological filtration

Pilot plant experiments were carried out using two O.75 m
diameter spun concrete pipes containing 2 metres depth
of 20 to 4O mm graded washed shingle  (Fig. 4).
The best ammonia removal achieved was 02.5% at a super-
ficial velocity of O.8 m/hr but increasing the treatment
rate gave lower removal efficiencies with only 4.7% re-
moval at 2.8 m/hr (Fig. 5). Temperature had a big effect
on removal efficiency which was approximately linear
between 6 and 12 C.  At - a 'nominal feedwater concentration
of 3 mg/1 N, 75% removal at 12°C declined to 51% removal
at 6°C.
Analysis showed that ammonia.was essentially converted
co nitrate.

-------
                            r-  592 -
                 • 0.71m
   irlc*
                                               Fig.  4
                                               Biological filter
                                               construction
                          PLAN VIEW OF
                          DISTRIBUTOR
                          nts.
                   Nominal raw water ammonia concentr. = 3 rog/1 N
                   Eaw water temperature range ,11 to 19°C
 ts
Flowrate
                                       zo
2S
Fig.  5  Effect of flowrate  on biological filter performance

-------
                        -  593 -
Biological sedimentation
The experimental work was  done  in two mild steel,  square
section tanks 4 metres high  and of O.58 m  area.  Raw
water entered through a  pipe directed downward into-the
tapered bottom and  flowed  upwards to  be removed by
launders at the top (Fig.  6). The initial ammonia concen-
tration in the raw  water was 3  mg/1.          ,
   Samph'ng
   taps
                                          Fig.  6
                                          Biological sedimentation
                                          tank  construction
              Bottom
              drain valve
                      n
In tne early experiments no seeding of  the  biological
sedimentation tanks was carried out and even  after  eight
months running at low upflow rates there was  no  floe
build up in the units. Ammonia removal  was  poor,  gene-
rally between O and 2O%. Only when sufficient fine  sand

-------
                         -  594  -

(5O-15O micron) had been* -added '-to-1' .form* a">detectabld "" (-r;'•'
fluidised bed did the ammonia removal increase .above 2O%,
Increased removal started  1O days after the addition
of sand and reached a peak of 85% after 28 days. There-
after it stabilised at around 6O%.
Sixty percent removal was maintained for 35 days' and
then the ammonia dose was reduced from 3 mg/1 to 2 mg/1.
The two filters immediately began to remove 100% of the
ammonia and this performance was maintained for the
duration of the test.
The biological sedimentation units were evidently sensi-
tive to raw water anmonia concentration but as the raw
water contained about 10 mg/1 of dissolved oxygen and
theoretically this is capable of converting 2.2 mg/1 of
ammonia nitrogen to nitrate, the units were clearly
working at full capacity - availability of oxygen being
the limiting factor.  (4.57 mg/0? is needed to convert
1  mg of ammonia nitrogen to nitrate but it was found
that the loss of dissolved oxygen in the biological
sedimentation units was slightly more than the theoreti-
cal figure by about 1 mg/1. It did appear, however, that
oxidation  of ammonia took precedence over other oxygen
demands and no substance used oxygen preferentially to
ammonia. A brief experiment during which pure oxygen was
injected into the feed gave improved ammonia removal.)
Effect"of temperature         '•
A fall in raw water temperature from 21 C to 4 C did not
affect performance although the performance of the bio-
logical filters deteriorated significantly under the
same conditions and over the same period. The higher
solubility of oxygen at lower temperatures is presum-
ably a contributing factor in offsetting decreased bio-
logical activity at lower temperatures (Fig. 2).

-------
                         - 595 -

Concentration of fluidised solids <    -t   • v_ • • >• -  •  -  ,
At one stage in the work  the  ammonia  removal efficiency
dropped from 10O% to 6O%. This was  shown  to be  due  to
a reduction in the concentration of fluidised solids.
(Fluidised solids concentration was measured as  the
percentage solids volume  after four minutes settling -
very  little further settlement occurs  after four minu-
tes.)  Ammonia removal was found to be  practically
zero for fluidised solids concentrations  less than  15.%.
Low water temperature, which  increases  viscosity, will
reduce fluidised solids concentration by  expanding  the
bed further than the same flow velocity at higher tempe-
rature .  This effect can  be eliminated  by keeping flui-
dised solids concentration above 35% as above this figure
changes do not effect efficiency.

Conclusions              .

Biological sedimentation was 'found to be  the cheapest and
most effective method for removal: of ammonia from river
water in the concentration range O.1 to 2 mg/1 ammonia
Nitrogen.  Unlike biological, filtration  and air strip-
ping it was not affected  by declining water temperature
in the range 21°C'to 4°C.
Effective operation on Thames river water  required a
fluidised solids concentration (measured  as percent vo-
lume settled after four minutes) greater  than 35% and:
an upflow velocity not greater than 25  m/h.
In the biological sedimentation process ammonia  is pre-
ferentially oxidised before all other oxygen demands but
removal efficiency is limited by the dissolved oxygen
content of the raw water  and  the process  is unlikely
to be able to remove much more than 2 mg/1 ammonia un-
less oxygen is injected or the effluent from biological
sedimentation is 'polished'  by' chlorination.

-------
                         - 596 -
(1)  SHORT,  C'.s.:.vw. -A-A  .':{--.  .-;  , :.:••.  . .-•  .'..-.•  • 	  '. o/.iv	;>  -:	
    Removal  of ammonia from river water
    Technical Publication TP 1O1 , Water Research  Assoc.  (1973)

(2)  SHORT,  C.S.
    Removal of ammonia from river water
    2.  Technical Report, TR 3, Water Research  Centre (1975)

(3)  NERETNIEKS, I. et al.
    Removal of ammonia from wastewater.by countercurrent
    stripping with air - an economic study
    Vatten  2£ (1973), 3, 269-8O

(4)  IONICS, Inc.
    The electro-oxidation of ammonia in sewage to nitrogen
    Project No. 17O1O EED, Report to Water Quality Office
    OSEPA,  July (197O)

-------
                         - 597 -'.  •

PURIFYING ACTION OF THE GROUND IN THE TREATMENT. OF DRINKING'
WATER                      '':.,''-'
H. KuBmaul
There are good reasons for u'sing ground water as the pre-
ferred source for drinking wa,ter supply; because of the
strong purifying action of the ground it is generally
hygienically unimpeachable and balanced, at least when it
comes from large-scale, porous ground-water systems with
intact covering strata.  No mention will be made here of the
difficulties in recovering the ground water from fissured
rock formations.

The purifying action of the ground is due above all to the
following factors.  When water penetrates underground it
undergoes a filtration process in which the solid particles
that can adsorb the water constituents are removed.  Harm-
ful material can then be eliminated in the ground e.g. by
adsorption and ion exchange on the mineral particles and on
the humus substance by precipitation in the form of sparingly
soluble or insoluble compounds, possibly by co-precipitation
with other substances, and organic material in particular
can be degraded by bacteria.

The effectiveness of the underground purification processes
is presented in this report on the example of ground water
enrichment with river water, in the light of experience
based on years of investigations at the river filtrate water-
works on the Lower Rhine.  The process for the supplement-
ation of the ground-water reserves by river filtration is
used extensively and successfully in West Germany and in
some neighbouring countries.  In this process the'infil-
tration of river water into the ground is promoted by

-------
                          - 598 -

                                                     1 .    V .. ,r .  ,.,,,-.
the formation of descending funnels through feed' Wells "in" :'" ""'	"'
the immediate vicinity  of the bank (1).   In the waterworks
studied the  feed wells  were only about 50 m from the river
bank  (Fig. 1).  Between the wells and the river are
situated other observation wells,  which  were likewise
included in  the study  [6/III  - 6/1].   The residence times
of the river filtrate underground are on average three  weeks,
but fluctuations between 3 and 46 days are possible, depending
on the water level of the river; The mean proportion of
river water  in the raw  water  passed on for treatment is
about 80%, fluctuations of 30-100% being observed depending
on the water level of the river (2).

In the discussion of the behaviour of the water constituents
underground  a distinction should be drawn between the
organic loading, since  biological degradation predominates
in the former case and  physico-chemical  processes in the
latter.
                        Forderbrunnen^Feed weiis/Puits
                           .  .   .   '.  . Bra6/I.
          oBr18/m
                      •Rhine-
      20  40  60 m
Fig. 1  Position of waterworks  I  on  the  Rhine

-------
                         - 599
Organic substances                .•",,, ..,---.,  ,.,.,,,    .
The dissolved organically bound carbon is a  measure
of the organic substance  content of a water (DOC = dissolved
organic carbon).  The mean values of two waterworks are
shown in Fig. 2, from which it can be seen that up to the
first observation well  [6/III]  in the immediate vicinity of
the bank of works 1  the DOC content has halved, and that no
further degradation  takes place during the following under-
ground passage.
     DOC
     ImgA)
      RhE HI R>G  (WHO
         1       11

20

10
A
COO




^
J?>

-
N»»

-



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1
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XI
^
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S1.
'2
^


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\l
Rhllin I feO  RhtoG
  I      11
50
w
30
20
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KMn,O4 -
kn^I)




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^J
>









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Consurnt-lr


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o
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Th
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mo
S.O.
°2
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-:
'£•
\f.
^"



^




^
T
?
1


n
K
      (*mil I teG  RhRoG
                II
RhlD 0 1 RoG  RhfoS
   I       It
Fig._  2   Variation of the DOC, COD, KMnOj^ consumption,  and  free
         oxygen during underground passage  (bank filtration)  in
         two Rhine waterworks
         (Rh = Rhine;  III,II,I = bank filtration wells 6/III-I?
         Ro = raw water of the feed gallery;  G = ground water)

-------
                        - 6OO -'

Indications on the content of degradable organic substances
in a water can be obtained from the chemical oxygen demand
(COD) and the KMnO^ consumptiqn.  It'is found that the mean
COD content of the waterworks in the 'immediate vicinity of
the bank has also decreased by 50%, but the substances in
question undergo an additional decrease during their under-
ground passage.  The KMnO. consumption falls by 68% in the
immediate vicinity of the bank and undergoes no further
change(Fig. 2).

The microbial degradation of organic substances is associated
with a consumption of oxygen dissolved in the water.  Accord-
ingly, the lowest amount of oxygen in the water is found
immediately after the loaded water has penetrated under-
ground,  and the O2 content then rises again (Fig.  2).   If
the dissolved oxygen is insufficient for biological oxi-
dation of the degradable organic substances, oxygen is
released from inorganic compounds, e.g. from nitrates, which
can lead to reducing  anaerobic conditions in the ground
water, with all their adverse, effects on the nature of the
water.

The elimination rates for the underground passage and the
subsequent treatment of the drink'ing water with ozonization
and active carbon filtration, calculated with an allowance
for the mixing ratios of river water to ground water and the
respective contents, are shown in Fig.' 3.  Further details
of the calculations can be obtained from (3).

In Fig.  3 it can be seen that in- comparison with the under-
ground passage the drinking Water•treatment only reduces the
DOC content further by 8%, the COD -content by 20%, and the
KMnO* consumption by 11%.      •.••,..

-------
                         - 6O1 -


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COD


KMml'V

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l3'^ '
-.M
*20 Ahdtfung'{*/»if
         Review  of  the  concentration changes  of some water
         constituents during  bank  filtration  and drinking
         water treatment   . • .   '  .
          (KMnOi^-V  = KMnOi^ consumption j   Ges.H. = total hardness;
           Karb.H.  = carbonate hardness)
In addition to these sum parameters for the organic loading
only one other group of: substances- should be characterized
in closer detail, this be ing > the, organochlorine compounds.
Since organochlorines do not normally occur in nature, they
indicate an anthropogenic o-rigin and can therefore serve as
a pollution indicator.  Only about 50% of the readily vol-
atile organochlorine compounds is. . eliminated during the
underground passage, and after the drinking water treatment
a -residual content of about 20% is still present. ;  Somewhat
more favourable relationships are found for the difficultly
volatile organochlorine compounds;  here about 70% of the

-------
                         -  602  -

initial content,.'is.••.removed during the underground, passage
and  a  further 20% or so during the treatment of drinking
water  (Fig.  4)   (4f5).
               Organochlorvtrbindungtn
               Orqanochlorine compounds
               Composes organo-chlores
           leichtfluchtig
  schwerfluchtig
1
50
40



30

20-

10
n
easily volatile t
pgA tr3s volatlls











77,
^
' /
'''*.'
. /

'' ''•
N
'•/'.
' 'X
VX






___.







20-





10-



	 n
difficultly vola
pg/l ncu volatlls











r^n
', S
'''^
''\
/ f
'''/',
\
J






— , ,
"I ".
        Rh Ro T
Rh Ro T
 Fig.  4  Changes in the  contents of easily  volatile and
         difficultly volatile extractable organochlorine
         compounds during underground passage  and drinking
         water treatment
         (Rh = Rhine;  Ro = raw water;  T = drinking water)

The behaviour  of other groups of  compounds in the ground
was also determined.   This is described in greater detail
elsewhere  [6,7].   However, it was found almost invariably
that the greatest purifying action took place in the
immediate vicinity of the bank  and that the purifying  effect
of the ground  is very high in comparison with the subsequent
treatment of the water.                ...                -

-------
                              - 603 -

;.- ••'However,  if the individual substances are examined it is
     found that the retention capacity can fluctuate considerably
     with respect to the various components;  thus,  many components,
     even of similar composition, are retained very well,  while
     others can pass through the ground,  and  often  even through
     the drinking water treatment, suffering  practically no
     decrease  (5-7).

     Inorganic materials
     The concentration changes of the usual inorganic water
     parameters during bank filtration and drinking water  treat-
     ment are  described in detail in (3).   Here only a summary
     is given  (Fig. 3).

     Although  nitrate in the vicinity of  the  bank is/reduced to
     a quarter of its original concentration, in connection with
     biological oxidation of the organic  substances, the calcu-
     lated elimination rate for the underground passage is only
     26% because of the admixture of nitrate-rich ground water.
     After the drinking water treatment the nitrate returns
     approximately to the original content in the river water.
     This is not the case with ammonia and nitrite, which  are
     almost completely eliminated during  the  underground passage
     and drinking water treatment.  The orthophosphate content
     of the infiltrated river water is reduced to a residual
     content of,a few percent in the direct vicinity of the bank.
     The calculated elimination rate is 95% and is  only very
     slightly  altered during the drinking water treatm'ent.

     In the case of sodium and chloride no changes  take place
     during the underground passage and drinking water treatment.
     Potassium and borate show a slight.decrease of 15%, and in
     the case  of fluoride the elimination is  about  33%.  The
     hardness, given as CaO, MgO, total,  and  carbonate hardness,
     increases during the underground passage by 5-20% as  the
     water dissolves some mineral constituents.

-------
                           - 604 -,
The  elimination rates calculated  for some 'heavy metals
shown in Fig. 5.   It can be seen  that zinc, chromium,  and
iron are retained  almost quantitatively during the  under-
ground passage.  Elimination rates  between 50 and 71%  are
obtained for lead,  cadmium, and mercury.  Copper has an
elimination rate of 11%, while an increase of 17% is
established for nickel, and manganese is increased by a
factor of 3.  This  increase in the  manganese content is due
to dissolution processes in the ground as a result  of  the
weakly reducing conditions.  With the exception of  mercury,
manganese, and nickel,  no changes occur during the  drinking
water treatment.  Manganese is almost wholly precipitated
under the oxidizing conditions now  present, while nickel
increases by a further  85%, presumably on account of disso-
lution processes in the conveyor  and treatment plants.
  Pb

  Zn
  Cd
  Cr
  Cu

  Hg
  Ni
  Mn

  Ft
        I—ESS
I NXVSX
                            pr.^icTUru* tjoi l'p.teroriins!i-.issnoe
                            Ohar.o^ durl.nn urulnrarounci nssanni-
                            Variation lr*rs du P.ISR.VJO Jans IP sol
                            \m!i*rv:rv* bcl Trlc.kwflispraiifl'i'roltur.a
                            Variation lor* i!u traitp-i'nt i r 1'oflu
                                        Ovl
      -100
                 so
                                      so
                                                «100
                                                   (Thannr
                                                   Variation
Fig. 5   Changes in heavy  metal and trace  metal contents
         during bank filtration and drinking water treatment

-------
                         -  6O5- -   •

The numerical elimination rates given here are only valid
for the conditions on the Lower Rhine.  They cannot be
generalized since, as can be seen in Table 1, the heavy
metal concentrations in the raw water (second column) are
very similar to the natural background  (fifth column); they
therefore correspond to the concentrations of the individual
heavy metals in solution equilibria in the ground.
 TABLE  1  Average  1972  and  1973  values  for  the  dissolved  shares
         of  some  heavy metals  in  the river water  and  raw water
         of  waterworks I and II(yg/1)  as well  as  background
         values
Rhine
Pb 28
Zn 295
Cd 1.2
Cr 44
Cu 21
Hg 0.8
Ni 11
Mn 164
Fe 1132
Raw water
(= bank filtr.)
11
10
0.4
0.8
16
0.2
11
534
35
Change (%)
during under-
ground passage
- 52
- 96
- 60
- 98
- 11
- 71
+ 17
+ 301
- 96
"Background"
3
10
0.2
1
7
0.1
3
7
50
Summarizing, as regards the removal of heavy metals,  it can
be  stated that the elimination action of the ground under
aerobic conditions is so high that only those concentrations
are still present in the river filtrate that correspond to
the natural background values; the retention capacity
increases numerically with increasing loading of the  surface
water with heavy metals.

-------
                         - 606
The elimination of heavy metals is, however, partly revers-
ible.  If a change takes place in the redox potential in
the ground, or if larger amounts of chelate formers are
present, there is a danger that the heavy metals will re-
dissolve.  This danger is increased by the fact that con-
siderable amounts of heavy metals are bound in the sediments
and the suspended matter of the river, and these can also
pass into solution under anaerobic conditions.  This is
especially dangerous for river filtrate waterworks, since
the current water-treatment processes are not particularly
suited to the removal of heavy metals.

Summary
The results of the investigations indicate that the inorganic
and organic loading of river water is reduced immediately
after penetration into the ground by filtration, adsorption,
precipitation, or microbial degradation.  However, some of
the material is not retained during the underground passage
or the drinking water treatment.  The man-made foreign
substances are particularly important in the evaluation of
the safety of drinking water supplies.,

The penetration of highly charged river water underground
during ground water enrichment by bank filtration is
admittedly an extreme case.  However, taking into account
the low loading of harmful materials in precipitation water,
the present results also allow conclusions to be drawn on
the possible endangering of ground water in natural ground-
water formation.

-------
                      - 607 -
.(1). BMI-FachausschuB "Wasserversorgung und Uferfiltrat"
    Uferfiltration, Bonn 1975

(2) KUSSMAUL, H. , MUHLHAUSEN, D., BEHRENS, H.
    Hydrol. und hydrochem. Untersuchungen zur Uferfiltration
    Tell I: Chlorid, Borat und Uranin im Flufiwasser als
    Leitsubstanzen zur Ermittlung von Verweilzeiten und
    •Mischungsverhaltnissen bei der Trinkwassergewinnung
    durch Uferfiltration
    gwf-Wasser/Abwasser 118  (1977), 521-524

(3) KUSSMAUL, H., MtJHLHAUSEN, D.
    Verweilzeiten, Mischungsverhaltnisse und Veranderungen
    der Wasserbeschaffenheit bei der Uferfiltration am
    Niederrhein
    WaBoLu-Bericht 42 (1977)

(4) KUSSMAUL, H.
    Behaviour of Persistent Organic Compounds in Bank-
    filtrated Rhine Water
    Aquatic Pollutants - Transformation and Biological
    Effects, London  (1978), 265-274

(5) KUSSMAUL, H., FRITSCHI, U., 'FRITSCHI, G., SCHINZ, V.
    Leichtfliichtige Halogenkohlenwasserstoffe im Rheinwasser,
    "Uferfiltrat und Trinkv/asser.
    WaBoLu-Bericht 3^ (1978) , 75-85

(6) HEGAZI, M.
    Analytik und Verhalten von Phenylharnstoff-Herbiziden
    und deren Metaboliten bei Uferfiltration, Trinkwasser-
    aufbereitung und Bodenpassage
    Diss. Bonn 1977

(7) FRITSCHI, G., KUSSMAUL, H., SONNENBURG, J.
    Cholinesterase-hemmende Stoffe im Qberflachenwasser,
    Uferfiltrat und Trinkwasser
    WaBoLu-Bericht 4O (1976)

-------
                        - 608 -


BEHAVIOUR OF MICROPOLLUTANTS IN RIVER WATER DURING
BANK FILTRATION

G.J. Piet and C.F. Morra
1.  Abstract  .

The analysis of individual organic chemicals in water
of the river Rhine and of this.water after bankfiltra-
tion gives an idea of the behaviour of organic com-
pounds during passage through the soil.
Chemicals which pass into the drinking water supply are
selected, their maximum concentrations are listed and
a notation is made when they belong to a suspected group
of chemicals. Means are indicated to prevent drinking
water pollution by industrial compounds. Recommendations
are given for future research on substances which impair
the quality of drinking water derived from bankfiltered
riverwater.

2.  Introduction

Bankfiltered water can become important for the drinking
water supply in The Netherlands. In the year 1976 drinking
water was abstracted from groundwater (700 mlll.m )  and
from surface water (4OO mill.m  ) with the expected increase
of drinking water consumption more and more surface
water has to be used because of the limited availability
of local groundwater (1). In The Netherlands a use of
250 million m  bankfiltrated water for the drinking
water supply is foreseen which quantity could be extended
to 100O million m /year after the introduction of revised
structural plans. The advantages of bankfiltration such
as a reduction in the concentration of organic pollutants

-------
                        - 609 -
disinfection without the application o'f chemical oxi-
dation/ reduced influence of -calamities in the river on
the quality of the bankfiltered water when residence
times in the soil are sufficiently long, the fact that
the water is stored in a protected place where organisms
which can introduce unwanted metabolites cannot affect
the water quality, make it a good and non-expensive
water treatment system.
The information on bankfiltration is also of importance
to other infiltration techniques such as dune infiltra-
tion and groundwater recharge in combination with slow
sandfiltration. A close examination of the processes
which take place in the soil is of great value to select
additional techniques such as activated carbon treatment
necessary to produce wholesome and agreeable drinking
water when heavily polluted river water is used as raw
water source (2, 3).
The quality of drinking water derived from bankfiltered
Rhine water is affected by organic compounds, particular-
ly industrial chemicals which pass into the drinking
water supply.
Suspected chemicals or groups of chemicals have to be
considered in relation to the long-term effect on human
health and to the odour and taste of finished water. It
is important to have knowledge of the processes which
play a role in the reduction of organic chemicals during
passage in the soil. Chemicals which endanger the quali-
ty of drinking water from bankfiltered Rhine water have
to be selected.

3.  Organic chemicals in water of the river Rhine
    which affect the drinking water quality

In tapwater from bankfiltered sources more than 2OO
organic substances have been identified and subsequent-
ly quantitatively measured by analytical procedures based

-------
                         - 610 -
on a gas—stripping technique and a XAD adsorption
technique  (4). The analysis of Rhine water is carried
out by an  extraction technique with cyclohexane-diethyl-
ether and  by means of a static head-space method  (5, 6).
A capillary GC-MS system is used for identification.
Special attention is given to1 those chemicals which are
present in river water and pass during bankfiltration.
H thorough chemical analysis of tapwater from three
water production plants using bankfiltered Rhine water,
was made to select suspected chemicals.

The selection of compounds was made on the basis of the
following  criteria
- Experimental evide.nee of toxicity for man or animals  „
  including carcinogenity, mutagenicity and teratogeni-
  city (7)
- Identified in drinking water at relatively high  con-
  centrations
- Molecular structure closely related to other toxic
  or odour-intensive compounds
- A known  odour threshold concentration in water which
  is 1% or.less of the actual concentration  (8).
..In the investigated water plants only aeration and rapid
sand filtration is applied after bankfiltration. The time
of residence in the soil is at least 1OO days in all
cases.

4,  Organic chemicals in drinking water from bankfiltered
    Rhine  water after passage of the' soil

The selected organic chemicals analysed in finished
water from bankfiltered Rhine water in The. Netherlands
are listed in Table I. Though the concentrations of these
substances differ from place to place due to different
conditions of the soil passage, usually the same type
of compounds appears at different plants.

-------
                      - 611 -
Table  I              •     •    " ""
Selected organic chemicals in tapwater from bankfiltered
Rhine water in The Netherlands
(maximum concentration in ng/litre)
Component
chloro-ethers
Max, cone.
ng/litre
bis (2-chloroethyl)ether    3O
bis (2-chloroisopropyl)   3OOO
ether
chloro-benzenes
chloro-benzene             3O
o-dichloro benzene    •    10O )
m-dichloro benzene        1OO )
p-dichloro benzene        3OO )
e-trichloro benzenes.     3OO
chloro-methyl benzene       5
chloro-alkanes and
alkenes
chloroform                3OO
tetrachloromethane        1OO
1,2-dichlorethane         5OO
tetrachlorethene           5O
1,2-dichlorpropane        3OO
trichlorethene            5.OO
other chloro compounds
chloro aniline  (m,p)     1OOO
5-chloro-o-toluidine      3OO
tri(2-chloroethyl)      -  1OO
phosphate           .    .    ••."'-..• •
           suspected animal carcinogen
           'suspected animal carcinogen
           suspected animal carcinogen
                                           (continued)

-------
                      - 612 -
component
aromatic chemicals
Max, cone.
ng/litre
benzene .
toluene
'ethylbenzene
o-xylene
m/p-xylene
Co-benzenes
c^-benzenes
others
naphtalene
divinylbenzene
2-methylnaphtalene
acenaphtene
biphenyl
anthracene
pyrene
alkanols, aldehydes
C[--alkanols
Cg-alkanols
geosmin
dimethylbenzaldehyde •
cinnamaldehyde
others
present
1OO
30
30
1OO
1 OOO
10O

1OO
•' 30
30
30
10O
• 30
. ' 30

•.300
. • 10Q
•10
• . - , 1 ooo •
30O

oxygen-containing components
1,1-dimethoxy propane        1OO
1,1-dimethoxy isobutane ,   ,  30O
bis(3-methoxy-ethyl)ether    3OO
bis(2-methoxy-2-ethoxy)ether 1OO
methylisobutyrate            1OO
dimethyl acetophenon         10O
                                            (continued)

-------
                      - 6V3- -
 component         .     /Max.  cone.          ••'•". ' -.'."'"''"'
                         ng/litre
.triethyl phosphate       1000                    .,  ,
 tributyl phosphate        ,30
 3  unknown  compounds       300  - 1000

 It is  evident that  several industrial chemicals  are
 not fully  e'liminated even during long residence  times
 in the soil.
 Some lower esters and ethers,  which improve the  odour
 quality of water are present  after groundpassage.
 It is  not  fully understood whether bis(2-chloroethyl)—
 ether  is formed during groundpassage or not.
 Some aromates have  rather low odour threshold concen-
 trations  (such as naphthalene,  mesitylene etc.).  Toluene,
 xylenes, and  ethylbenzene are substances which may
 affect water  quality too.
 Polynuclear aromatic hydrocarbons are mainly presented
 by fluoranthene and,always in concentrations below
 5O ng/litre.
 The concentration of alkanes,  with exception of  C,. -
 alkanes was always  below the  1OO ng/litre level.
 An important  reduction of several chemicals can be
 reached if the residence times are sufficiently  long.
 For this reason a consideration of the analysis  of
 the river  water of  the Rhine  is of interest.

 5,  Organic chemicals in the  river Rhine

 The investigation of the water of the river Rhine is
 performed  by  the same instrumental analysis as the bank-
 filtered water,and it'became  evident that the following
 major compounds in the Rhine .water are almost com-
                             *.
 pletely removed during groundpassage  (Table II).

-------
                      - 61.4 -
Table II

Organic chemicals at concentrations > 1 jjg/litre 'in
Rhine water which were not detected in related tap-
water (9)
Component

nitrobenzene
o-nitrotoluene
m-nitrotoluene
p-nitrotoluene
di-nitrotoluene
p-nitro aniline
N-ethyl aniline
N.N.-diethyl aniline
amino-nitro toluene
diphenyl amine
m/p-chloro nitro benzene
o-chloro nitro benzene
m/p-chloro toluene
o-chloro toluene

methyl-tert.butylphenol
diethylene glycol diethylether
2,6-di(t)butyl-1,4-benzoguinone
2(methyl-thio)benzo thiazol
Concentration
(ug/litre)
1  - 10
10
 1
 3
 1
 1
 T
 1
 3
 I
 1
 1
 1
 1

 1
 1
 2
 1
It is of interest that some of these chemicals seem
to be rather persistent to microbial decomposition in
river water, they are eliminated, however,during passage
of the soil.
The chlorinated butadienes, chlorinated phenols and
cresols are not found as major contaminants in The
Netherlands. The same is true,for o-phenyl-phenol, p-
nitro-phenol, phenol, m-cresol and 2,4-dimethyl phenol  (1O)

-------
                         - 615 -

  Though a great diversity of industrial pollutants is
  present in Rhine water, only  a limited number passes
  into the drinking water in The Netherlands at concen-
  trations > 10 ng/litre.
  Several processes involving the reduction of organic
  chemicals during bankfiltration are similar to those
  during slow sand.filtration where mainly microbial
  decomposition modifies organic compounds. In 2 water
  treatment plants using slow sand filtration techniques
  after dune infiltration of river water, the following
  compounds were present in concentrations> 10 ng/litre
  (see Table III).
TABLE III  Organic chemicals in drinking water after dune
           infiltration and slow sand filtration of polluted
           river water  (concentrations  > 10 ng/litre)
  alkanes
  alkyl benzenes

  naphthalenes
  alkanols
  aldehydes

  ketones
  ethers
  esters
  phtalates
  organo-halogens
nitrogen-containing
compounds
sulfur-containing
compounds
CQ-CI. alkanes
toluene, C^-benzenes, C.-benzenes,
ethyl styrenes
naphthalene
C5-C_ alkanols
dimethyl benzaldehyde, cinnamal-
dehyde
acetophenone
1,1-dimethoxy isobutane
ethyl acetate, butyl acetate
dimethyl-, diethyl-, dibutylphthalate
tetrachloromethane,  trichloroethene,
tetrachloroethene,hexachlorobuta-
diene, chloro benzene, o-dichloro-
benzens, p-dichloro-benzene, bis-
(2-chloroisopropyl) ether

     : N-ethyl aniline

    . : benzo thiazole

-------
                          - 616 -
 A correlation' 'between' 's'ome 'dhemicaIs which  are;  not'''- •'•"•'•'-'•"•'
 completely removed by slow-sand 'filtration  and  bank
 filtration is evident.

 6.  Processes which play a role 'during bankfiltration

 Filtration of particulate matter with adsorbed  organic
 chemicals such as PAH's, PCB's, pesticides  and  in-
 secticides, high-boiling hydrocarbons leads to  the reduc-
 tion of the concentration of  several organic  contami-
 nants during passage of the soil. Adsorption  and  ion
 exchange will also take place.     .
 In the case of precipitation  of e.g.carbonates  and sul-
 fides co-precipitation of organics•can occur. This is,
 however, a slow process where  the equilibrium  between
 dissolved and precipitated substances is  time-dependent  (11),
 Chemical reaction such as hydrolysis can  occur  also.
      Microbial decomposition,under aerobic  and  an-aerobic
conditions, the presence or nitrates for the additional sup-
ply of oxygen affect for a g'reat deal the  decomposition
effect of organic chemicals in the soil. The degradability
of compounds which decreases with the increasing number of
halogen atoms and depending on the type of halogen atoms
oftentimes determins  the fate of organics in  the  soil  (11).
Several organo-chlorine compounds which are  not  readily
adsorbed seem to survive. For  this reason  after-treatment of
bankfiltered water should be- performed with  strong adsorption
procedures such as activated carbon treatment. But even when
this procedure is applied after ozonation of  bankf iltered water
substances such as chloroaniline,- .chloroform,  dichloro-
benzene, tetrachloromethane, 1, 2-dichJLoroethane,dibutyl-
phthalate,C., benzenes, C-o alkanes, triethylphosphate
and some unidentified  substances were.not  completely
removed during the processes-  in a drinking water plant.
Water sanitation programs have to be installed to  im-
prove drinking water quality while structural  plans to

-------
                        - €17 -  ,;  . .

increase residence time in the gr,o,und, .are also necessary.
Recharge of groundwater .leading to a certain dilution
of pollutants is of assistance to provide a good quality
drinking water from polluted surface water.
Pretreatment processes/ such as .f locculation which re-
moves organic material but more so ozonation which im-
proves the biodegradability. of organic substances and
makes these better adsorbable, are recommended to improve
the quality of bankfiltered water.
In the case of possible mobilization of chemicals or a
certain degree of "saturation" of the ground after pro-
longed use of bankfiltration, model studies as will be
performed at the National Institite for Water Supply (11)
are of assistance. The fate of chemicals during passage
of the soil can be better predicted when more insight in-
to the processes taking place in the soil is obtained.

7.  Conclusions and recommendations

- Bankfiltration is a safe, inexpensive and reliable
  technique of water treatment
- Suspected chemicals which "break through" have to be
  monitored and proposed for surface water sanitation
  programs
- Structural plans have to be developed to attain optimal
  residence in the ground if possible
- Some fundamental studies.of.mobilization of chemicals
  have to be set up
- Additional treatment systems, "necessary to produce a
  wholesome and agreeable type- of drinking water are
  necessary when bankfiltered Rhine water is used for
  the drinking water supply
- The introduction of pre-treatment of bankfiltered water
  by flocculation and/or ozonation can be of great impor-
  tance.

-------
                      -  618  -
(1)  S50ETEMAN,  B.C.'J. ...'..'.        ,.  ,         •  ,   ,,  ,
    Kwalitatieve beschikbaarheid van grond- en
    oppervlaktewater        "   *  ,
    H2O  _8 (1975) ,  4, 75-81

(2)  SONTHEIMER H.,  MISSING,  W.
    Xnderung der Wasserbeschaffenheit bei der Bodenpassage
    unter besonderer Berticksichtigung der Uferfiltration
    am Niederrhein              -       •
    Gas-Wasser-Abwasser 57 (1977) ,  9, 639-645

(3)  ROBERTS,  P.V. et al.
    Ground-water recharge  by  injections of reclaimed water
    in Palo Alto                 '••••.
    Civil Engineering Technical Report No. 225, Dept.  of
    Civil Eng., Stanford  University, Stanford, Ca. (1978)

(4)  ZOETEMAN,  B.C.J.
    Sensory assessment and chemical composition of
    drinking water
    Thesis, National Inst. for Water Supply, Leidschendam,
    The Netherlands

(5)  PIET, G.J.  et al.
    A fast quantitative analysis of a wide variety of
    halogenated compounds in surface- drinking- and
    groundwater
    Internat.  Symp. on the Analysis of Hydrocarbons and
    Halogenated Hydrocarbons in the- Aquatic Environment,
    Hamilton,  Ontario,  Canada, May  23-25 (1978)

(6)  PIET, G.J.  et al.
    Determination of very volatile  organic compounds in
    water by  means  of direct head • space' • analysis
    Analytical  Letters, A11. (1978),~5, 437-448

(7)  Drinking  Water  and Health
    At the request  of and funded by the U.S. Environmental
    Protection  Agency,  National Academy of Sciences,
    Washington, D.C. (1977)

(8)  Van GEMERT, L.J., NETTENBREIJER, A.H.
    Compilation of  odour  threshold  values in air and water
    National Inst.  for Water-Supply; Central Inst. for
    Nutrition and Food Research,  TNO, Zeist, The Netherlands
     (1977)

-------
                       - 619 -
 (9)  MORRA,  C.F.
     Internal  communic.,  National Inst.  for Water Supply,
     The  Netherlands  (1978)

(10)  SMIT,  Z.
     Determination  of  some  phenolic compounds  by concen-
     tration on XAD resins  and T.L.C.
     Internal  communication,  National  Inst. for Water
     Supply, The  Netherlands  (1978)

(11)  HARMSEN,  K.
     Column  and adsorption  experiments to  be performed for
     the  examination of  the quality of groundwater in
     The  Netherlands  (in Dutch)
     Internal  communication,  National  Institute for
     Water  Supply,  The Netherlands (1978)

-------
                       - 620
EXPERIENCE WITH THE REMOVAL OF MICROIMPURITIES IN SLOW SAND
FILTERS
K. Schmidt
The behaviour of an impurity in water and the degree of its
elimination in a biologically active filter are determined
primarily by the following factors:

1.    Chemical and physical properties of the impurity
      material.
2.    Concentration, loading duration, and origin of the
      impurity.
3.    Biological assimilability or persistence of the
      material.
4.    Concentration and nature of the animate and inanimate
      organic charge of the filter material.
5.    Possible reactions of the unwanted substance with
      other substances present in the system.
6.    Functions of the active agent of the unwanted substance.

A number of other material properties and mechanisms  relevant to
elimination could be added to this list, but these are
mostly of secondary importance.

As regards its construction and the reactions taking place in
it, a slow sand filter has a very complicated structure, and
the individual factors are connected to one another by
numerous interrelationships and regulation circuits.  It is
therefore difficult to trace the exact course of the elimin-
ation of an unwanted substance in detail and to interpret it
satisfactorily from the scientific point of view.  The
empirically established performance of a system is difficult

-------
                        - 621 -
to fit into known patterns and its applicability to other
times or to other places is limited to at least an order of
magnitude.

The principal factors in 'the elimination cf impurities from
water, given in the preceding list, will now be considered
in closer detail.

1.    Chemical and physical properties of the impurity
      material
The chemical and physical material properties that have a
bearing on elimination in biological filters are as follows:

1.    Hydrolysability.
2.    Solubility in water.
3. '   Polarity.
4.    Volatility.
5.    Molecular structure.
6.    Molecular size.
7.    Electrical charge.

It is generally known that' polarity, solubility in water and
molecular size are factors decisive for the adsorbability of
a given compound.  The molecular structure determines the
compound's degradability, and the electrical charge and the
charge distribution determine the behaviour with respect to
other dissolved and undissolved constituents.  Different
materials vary strongly in these properties, giving rise to
correspondingly different patterns of behaviour.

2.    Concentration, loading duration, and origin of the
      impurity
The significance of the loading duration and concentration
for "the elimination of an unwanted substance in biological
filters is presented in .Table 1. Normally the unwanted

-------
                       - 622 -
materials are present in surface waters as a permanent more
or less uniform loading and usually in low concentrations.
If the substances are degradable, they are removed effi-
ciently by the biological action of a slow sand filter.
However, many persistent substances break through, usually
after a shorter or longer delay.  In the long run only that
fraction is removed which is removed from the system with
the topmost sand layer when the filter is cleaned.
TABLE 1  Significance of the duration and concentration
         in loading with undesirable substances for
         their elimination in biological filters
Type of loading
Continuous loading
Relatively low
concentration
(e.g. waste
water pipes)
Sudden loading
Relatively high
concentration
(e.g. transport
accident)
1
Material properties
readily degradable
difficultly degradable
persistent , adsorbable
persistent, non-
adsorbable
readily degradable
difficultly degradable
persistent, adsorbable
persistent, non-
adsorbable
Behaviour in
the filter
good elimination
good elimination
restricted elim-
ination
no elimination
anaerobiosis
inital break-
through
good elimination
no elimination

-------
                        - 623 -
In the case of sudden  loadings, e.g. after  accidents,  the
unwanted materials are present for only a short  time but
usually in relatively  high concentrations.   Readily
degradable substances  cause an intensified  oxygen  consumption,
which can give rise to anaerobic conditions in the system.
Sudden and heavy loadings with impurities that can only be
degraded after an adaptation pass through the filter in so  far
as they are not very readily adsorbable.

Short-term increases in  the concentration of persistent
adsorbable substances  are well intercepted  and often
pass through the filter  with a long time delay, the concen-
trations being frequently reduced by two to three  powers of
ten.

Table 2 shows the behaviour of biogenic impurities  in bio-
logical filters.  Many organic substances are formed by the
microorganisms in the  water and in the slow sand filters.
Even in main canals loaded with sewage more than half  of
the organic carbon can be of biogenic origin.  It  is known
that many of the substances responsible for undesirable
taste and smell of the water are released by algae and
actinomycetes.  Some excreta are highly stable as  metabolic
end products in the given system.  They can also pass  through
biologically active filters and cause serious problems in
the production of drinking water.

3.    Biological assimilability or persistence of  the
      material
Various types of biological degradation in  filters are shown
in Table 3.  Most organic materials are bio-degradable.  If
they can be assimilated  by many bacterial species, their
elimination will be spontaneous and far-reaching.  Sometimes
a necessary adjustment of the intracellular metabolism is
responsible for a certain delay in the degradation (enzymatic
adaptation).  A longer "running-in" time is needed

-------
                        - 624 -  >


if the substance concerned can only be utilized ,by, a sjnall
number of specialized organisms whose proliferation is
initiated by the impurity substance itself.


TABLE 2  Behaviour of biogenic impurities  in biological  filters
Origin Material properties
,
Normal plankton
content
.
Destruction of
a mass
population
readily degradable
difficultly degradable
persistent, adsorbable
metabolic end-products
persistent, non-
adsorbable metabolic
end-products
readily degradable
difficultly degradable
persistent, adsorbable
metabolic end-products
persistent, non-
adsorbable metabolic
end-products
Behaviour in the
filter
good elimination
good elimination
restricted
elimination
no elimination
anaerobiosis
initial break-
through
good elimination
no elimination

-------
                      - 625 -
       TABLE '3'  Varfous "types of biological
                degradation in filters
       Spontaneous degradation without adaptation
       by several species
       Slightly delayed degradation after enzymatic
       adaptation
       Delayed degradation,  only by "specialist"
       bacteria
       Slow degradation as a secondary metabolic
       reaction
       Final degradation to  inorganic end-products
       Incomplete degradation only as far as org-
       anic "refractory" end-products
       No degradation of persistent organic materials
Many harmful substances can only be transformed in secondary
metabolic processes, the microorganisms involved then
requiring other organic materials for their basic nutrition.
Their proliferation therefore cannot be controlled by the
harmful substance.             .  '

Not all degradable substances can be mineralized in bio-
logical filters to inorganic end-products.  Under certain
conditions biologically stable metabolic products are formed,
which can only again become assimilable after far-reaching
changes in the medium (e.g. a shift of the redox potential).
4.    Concentration and nature of the animate and inanimate
      organic charge of the filter material
Both the biological degradation and the adsorption processes
take place predominantly in the top few centimetres of the
filter bed, in which layer the suspended detritus is fixed
and the autochthonous filter fauna and flora develop most
intensively.  Both are absent in new filters and must be

-------
                        - 626 -
established in the course of a  fairly long running-in time.
In filters  exposed to the light algae pl'ay an essential part
in the  elimination of undesirable materials.

Pig.  1  shows schematically the  phases involved in the
substance conversion on the example  of the degradation of a
readily soluble compound: the granular skeleton of the
filter  material, the accumulated clay minerals, the bio-
logical population of the filter material consisting of
algae,  bacteria, and fungi, the embedded suspension material,
some of which consists of particulate organic carbon, the
water phase,  and the harmful material degraded by bacteria
with the aid of nutrients, a harmless degradation product
being formed in this case.  The arrows indicate the inten-
sity of the material transport.  It  should be emphasized
that biological filters are self-regulating in response to

                  Tine
|j~]  Mater     IfC^vy-lAlgae, bacteria [p
^TJ^ Suspended natter      Clay Minerals  A Unwanted aubatence'

           Nutrient     Jj^ Degradation product
                                  Fig.  1
                                  Degradation of a readily
                                  soluble substance
                                 Filter material

-------
                        - 627 -
 increases in the concentration of degradable materials  in
 the raw water:  the microorganisms which perform  the
 degradation proliferate and thus provide additional
 adsorption sites and increased degradation  capacity.

•'Fig. 2 shows the material transport for the case  of a sudden
 loading with a non-degradable, readily adsorbable substance.
 This is fixed mainly on the organic material in the topmost
 layer of the filter bed.  A small proportion not  fixed  here
 can also be fixed deeper in.
                                      Fig.  2
                                      Sorption  after short-
                                      lasting loading of
                                      unwanted  material:
                                      persistent,  well-
                                      adsorbable
    Water
    Suspended
    matter
  Algae,
,T "bacteria
unwanted
material
KiO<
Filter
material
Clay
minerals
 If the loading with persistent harmful materials  continues
 for a longer time, the conditions  in the  upper  layers
 approach a state of equilibrium with a corresponding concen-
 tration of the material  (Fig.  3) .   The region of  particu-
 larly intensive fixation of harmful material  is gradually
 displaced in the direction of greater depth.  When layers

-------
                        -  628
 with little or no biological growth have been reached, for
 many materials the adsorption capacity of the solid phase
 decreases and the passage through the filter bed is con-
 siderably accelerated.
                                       Fiq. 3
                                       Long-lasting loading
                                       with a persistent,
                                       readily adsorbable
                                       substance
   Water
   Suspended
   matter
-i Algae,
^ bacteria
 Unwanted
 material
Filter
material
Clay
minerals
If the contamination of the raw water  decreases  the material
equilibrium between the water  and  the  solid  phase  is  per-
turbed and the material is desorbed until  a  new  equilibrium
has become established  (Pig. 4) .   Desorption processes  in
the case of fluctuating water  quality  sometimes  result  in
higher concentrations of unwanted  materials  at the filter
outlet than in the raw water itself.
If the biological growth suddenly dies off and decomposes,
the adsorbed or accumulated harmful substances are once more
released  (Fig. 5).  This can happen when, e.g. iron bacteria
die as a result of an oxygen deficiency.  The best example,
however, is the release of harmful materials from decomposing
algae, with high enrichment factors for many substances.

-------
                         -  629 r
[•  Water
I Suspended
I  matter
             rrrn Algae,  ::
             '•'':'' fcapteria
               •-Unwanted  •
               material
»• Filter
-! material
S" Clay
1  minerals
                                           Fig. 4

                                           Desorption after
                                           temporary  loading
                                           with a persistent,
                                           well ad'sorbable
                                           material
                                           Fig. 5

                                           Release of  unwanted
                                           materials on destruction
                                           of the biological growth
                                           due to a change in the
                                           medium
rrjTi  Suspended
H^J  matter
                  bacteria   i'""'1  material
                Unwanted  ~   ^^  Clay
                material  	minerals

-------
                       - 630 -
The topmost  zone of the  filter,which  is particularly bio-
logically active, plays  a major part  in all these reactions.
5.    Possible reactions of the unwanted substance, with
      other substances present in the system
By reacting with other water constituents an unwanted
substance can be removed from adsorption equilibria primarily
set up and be fixed more or less finally.  Examples of this
are the sulphide precipitates of some heavy metals under
anaerobic conditions and the precipitation of manganese as
manganese dioxide or of iron as the hydroxide.

Another manner in which a substance can be removed from an
equilibrium between the liquid and the solid phase is by
complexing with natural or anthropogenic complex formers.

The active accumulation of harmful substances within the
cells of microorganisms can also be viewed in this way.

6.    Functions of the active agent
Many constituents of water exert an inhibitory or promoting
action on reactions of other substances.  Phosphate promotes
the proliferation of bacteria without increasing the loading
of the organic matter (1).  The degradation of higher hydro-
carbon loadings can be accelerated by the addition of bound
nitrogen (2).   The addition of growth substances, e.g.
vitamins, can also enhance the degradation.  Many substances
stimulate bacterial activity at low concentrations and
inhibit it when their loading is higher.

As a biological system,  a slow sand filter is highly sus-
ceptible to certain influencing factors, yet on the other
hand it can be very adaptable.  The physical filtration
processes taking place within it provide an additional

-------
                        - 631  -
security.  Thus, in  the  case  of a sudden loading with'"a
bacterial substance,  its concentration can be reduced to
such an extent in the upper filtration layer that the lower-
lying regions of the filter can still fulfil their degrad-
action function.  This is the case,  for example, in the
addition of chlorine to  combat algae.
             Ruhr water
               inflow
                    prel.  mter
       1,50 m
       0,50m
        2.00 tn
        0,8Drn
t-?.tti°AS«>>.o.sM
%*°'V!*&S' c'
;«*«ravel ,?°j,|B^ c2
  Fig.  6   Preliminary filter - main filter system
           (experimental ground-water plant)
It is not  feasible  to illustrate systematically all the
above-mentioned  processes by quoting relevant experimental
results.   The  publications of the Dortmund Water Research
Institute  over the  last 15 years should be consulted for
this purpose.  Some examples,- most of which were obtained in
the experimental plant shown in Pig. 6, can, however, be
given to clarify the above statements.

-------
                         - 632 -'
  In  Fig.  7  it can be seen how after a trial with additions
  of  cadmium strongly increased cadmium concentrations were
  produced in the wash-out phase by sudden  destruction of the
  algal  population right up to deep regions of  the f,ilter bed
  (3).   This demonstrates the high rate of  fixation in algae
  during the introduction of cadmium.and remobilization of the
        t
  fixed  cadmium when the algae die off, as  well as the release
  of  complex formers from the algal material, which prevent
  renewed  fixation of the cadmium in subsequent passage through
  the filter.
   ppbcd in the water
   Amount added
                  Death of algae
                  in  the filter
                        •X
 ,_,,_- °~T, Ubers'qu
f    *—0.1mTiefe
                  x—0,25m Ti«fe   •~3,OmTiefe
                  •— I.OmTiefe
                                    f—  Fig. .7
                                         Remobilization of
                                         cadmium on death of
                                         an algal population
Oberstau = upper water;   Tiefe = depth;  Tage = days

-------
                          - 633 -
 The preferred fixation of microimpurities  in the organic

 material  of  the upper filter layers  is  demonstrated in

 Figs.  8 and 9.
                                      2O    3O   41)   SOppn
Fig. 8  Heavy metal enrichment in the filter  sand  in
        long-term addition experiments
  0 cm
                                  PCB

        10 20  30 40 50  SO  70 80  90  100—•/.
                  Sandfilter

                  2400 ppb £ 100 %
             Discharge
Fig* 9  Percentage decrease of CLOPHSN A 30 against  filter
        depth at  the  end of the trial? experimental  ground-
        water plant

-------
                         - 634 -
 Fig.  8 shows the  enrichment  of  lead,  cQpper,  zinc,  and
 chromium  in the upper  sand layers  after heavy  metals have
 been  introduced over a period of several months (4).

 Fig.  .9 illustrates the PCS content of the filter sand in a
 dosage trial after a three-year  wash-out phase (5).   Even
 after this long time the greater part  of the fixed PCB is
 still present in the top few  centimetres of the filter bed.
 It is also clear that  when the slow sand filters are cleaned
 a considerable fraction of the harmful substances introduced
 is removed from the system.
 The illustrated adsorption processes  can also delay the break-
 through and even out the sudden loadings occurring in the raw
 water to  a considerable  extent, even  over relatively short
 sections  of the filter bed.  Fig.  1O  represents,  on the
 example of hexachlorobenzene,a break-through delayed by
 4 weeks with  a reduction of the concentration by  two powers
 of ten  (6).

 Hexachlorobenzene  ng/l
 10000
 1000
  100
   10



Fr-A
? / -,-
/
0*0

i
A\
A
/\


*.
/ •«
/ i
'
\


Cl% C.
Cl \ ff~C{
cf ~ci














*
1 "^V i


1
l
1
--— v\

~-\
0
•V




£= Beginning of addition
E= End of addition


Preliminary filter inlet
Preliminary filter outlet 	
Main filter outlet



!
i
«>-0
*»0
S*t:


,: 	 -^-- r-v.,.* v-._

i :



limit

defection Z
•
J
/
i » *-.


ng / 1 % ._ 	 —


\ \
-•*_.

"V.
              10
20
30
50
Days
Fig. 1O  Delayed breakthrough of hexachlorobenzene
         in a preliminary filter/main  filter  system

-------
                         - 635 -
 Fig.  11  shows the course of  the  concentration as a function
 of time,  at various depths in  the filter, after a sudden
 copper addition (4).  It is  clear that the concentration
 decreases with increasing depth  and the loading duration
 becomes longer.
PPb Cu in the.water
    fDosage)
0,10 >»
   0,25
            \  \   \
     0,40 10
\ \ J**~ " . •' ' ' "-^
\ \s ••" •-
\ /-•"•-• 	 •: —- 	 	
\ f ••--.::•- 	 -:- • : - - 	 -• :• i:"::"

> ... .*-...


rtl
\
      $
               \   \   \   \   \
            \   \   \   \   \  \   \
         1,00 '«
                     \\v\\   \
             3,00
                  \ 	\   A—;
                  \!  \   \
                  ..N   \    \
                      10   20   30  40   SO
    11  Behaviour of a  sudden loading of copper in  slow
        sand filtration

-------
                        - 636 - -. \,-v
In Table 4 are listed some of the organic substances that
have been shown to occur in anaerobic breakdown of algae,
their occurrence, and their behaviour in various trials.
As the third column shows, most of these materials could
also be found in surface water of the Ruhr.  The elimination
rates in column 4 were different in the slow sand filter
model.

The formation of palmitic acid, stearic acid, and methylamine
in the upper water of slow sand filters with algal growth
could be detected.  The last column of the table shows the
results of an experiment in which an algal suspension was
added to the filter.  Palmitic, hexadecatetraenoic, and
stearic acids, tyramine, and methylamine were released in
such quantities that their concentration in the filter dis-
charge was even higher than in the upper water  (7).

On the addition of many heavy metals a clear stimulation of
the bacterial activity could be observed.  Fig. 12  shows
the variation of the colony counts for two experiments in
which mercury was added in different concentrations (8).  In
the case of the lower mercury loading the increase of the
bacterial count in the upper water of the filter can be seen
in the right-hand half of the figure, together with the
increased bacterial content in the discharge from the prelim-
inary filter.  The discharge from the main filter shows no
reaction, but a reaction can be clearly seen in the left-
hand half of Fig. 12, relating to 10-times greater mercury
loading.   In the upper water'of the preliminary filter the
bactericidal action of the mercury is here clearly evident,
at least in the initial stages.

-------
TABLE 4  Occurrence and behaviour of fatty acids and amines from, algae
         in slow sand filtration

Palmitic acid
Ilexadecatetraenoic acid
Stearic acid
Linolenlc acid
Cadaver ine
Putrescine
Tyramine
Methylamine
Release on algal
degradation
in vitro
(anaerobic)
4
44
4
44
44
44
++
•1-
Detection
in the
Ruhr water
+~<1 ug/1
44A.1-20 ug/1
44
+
4
4+
NN
( + )
NN
44
Elimination on
•slow sand
filter model
Model expt.
with high
loading
3O - 60 %
0 %
30 - 5O %
O %
99,5 %
99,0 %
O' %
50, 0 %
Concentration
increase in
upper water
of filter due
fco algal
excretion
(+>
-
+
-
-
-
_
+
Concentration
increase in
filter due to
degradation in
filter dis- -
charge in the
case of loading
with algal
cells
+
.4+
4 '
-
-
-
44
+4

-------
                           - 638  -
            ——— Preliminary filter outlet
                 Upper water of main filter
                 Main level outlet
              5     (   10
        ABOUnt „ 1 50 ppb Hg
15 "Days
                                        Bacteria/ml
                                        105-
                                        10'
                  uoooo Inlet
                  	 Upper w&ter of  	
                  ~-—— preliminary filter
                  ——-—• Preliminary- filter outlet
                  —— Upper water of main filter
                  •	 Main level outlet
               Amount isppb HQ'
                 Rlter A
                       Fitter C
Fig.  12   Effect of  loading with mercury on the bacterial
          count in the  preliminary gravel filter and  the
          main sand  filter system
Fig.  13 shows  that small additions of indole and skatole are
eliminated in  the preliminary  filter after a running-in time
of  a  few days  (9).   At the same  time a fall of  the oxygen
content in the preliminary filter  discharge is  observed,
which cannot be explained by degradation of the brganic
substances added.   Their complete  oxidation would have
required less  than  0.5 mg O2/l.  A stimulating  action on
other mineralization processes must therefore be assumed.

Polarographic  determination of the heavy metals  with  mainten-
ance  of definite pH permits a differentiation of heavy
metals  bound stably and weakly in  complexes (Fig.  14).  As
the pH  decreases, increasing amounts  of  heavy metals  are
detectable in  the Ruhr water (10).

-------
                -  639  -
a) Indole
[Ha/iJ
»•
20-
10-

b) Skatole-
iHS/lj
w-
5-
•
c) 02
[ma/1]
5-
djNO,
, .15
[mg/1] -
10
5-
0,
t) P04
[mfl/tj
l(H
0,5
0.
Fig. 13
fW\T
A y
r*
jfs V • 	 • Haw water
l|i>^V x 	 -x Preliminary filter outlet

* 	 * Main filter outlet
r A /•
A v .
uv
\ / \
\ /

\ 7 • / '
\ A /
X / \ /
N. / \ / Tank
^v / \f containing
* - * '„.. 	 .,_ 	 	 
-------
                           - 640.-
K/uAl
  8-
  0,7-
  Q6-
  0,5-
  03-
  0,2-
      1   Untreated Ruhr water
                      (pH65
2  Acetate-buffered Ruhr water {pH 7}

3  Acidified Ruhr water     (pH 1!
          -0,7   -0,6   -0,5   -Q4   -Q3  -Q2  -0,1  E(V)
Fig.  14  Determination  of labile  (Curve  2)  and total
          {Curve 3)  Cd-Pb-Cu by means of  DPASV.
          Electrolysis time 18O sec.
 According to  Table 5, when  the  loadings of the  Ruhr are
 relatively  low,  lead is found to be 100% stably bound and
 cadmium to  be 100% labile.   Both elements were  completely
 eliminated  in a  70 cm long  experimental biological column.
 Copper and  zinc are present  in  higher concentrations and in
 both cases  only part of the  metal is labile.  As  expected,
 the labile  copper is completely removed in' the  filtration.
 Only 50% of the stably complexed copper could be  removed.

-------
                         - 641 .-
TABLE 5  Proportions of  stably  and weakly  bound  heavy metals
         in river water  and  filtrate

Raw water
Filtrate
Elimination
PPB
»
PPB
«
%
LEI
stably
bound
1,3
1OO.O
<0,2
-
10O,O
vb
labile
<0,2

<0,2
-

CO!
itably
bound
11,O
8O,O
7,O
1OO,O
50, 0
'PER
labile
3,5
2O,O
< 2,0
-
1OO,O
Ztt
stably
bound
60,5
55,0
<2,0
-
1OO,O
1C
labile
49,5
45,0
5,5
1OO,O
9O,O
CAD^
stably
bound
<0,1

<0,1
-

ilUM
labile
0,6
1OO,O
<0,1
-
1OO,O
A different situation is observed with zinc.  In this case
the stably bound fraction is completely removed and the
labile fraction only to the extent of 90%.  From this it is
evident that the stability of the heavy metal complexes
does not allow any general rules to be formulated about
their elimination.  The individual elements behave very
differently, according to their type and their concentration
(10).

In conclusion, some further indications may Ije given on the
efficiency of continually loaded plants with respect to
microimpurities.  The investigated water-production plant
consists of a preliminary gravel filter, a main sand filter,
and a 50-m long soil passage, which is completed in 1-2 days
(Fig. 15).
The DOC shown in Fig. 16, measured with Maihak apparatus,
is reduced on average by about 50% (11).  However, the
figure shows clearly that the elimination rate of the organic
carbon, contrary to expectations, is better during the winter
months than in the summer.  This can be explained by loading  .
with biogenic substances from algae.

-------
                               - 642 -
                  -1 ! ? i>rw.'-.*~^5 r"-"'.";l"*"'V
               Ruhr gravel and shingle
                                                            L-oc- '" •' '« - » '" * ~.-J
                                                               6 •  .x
                                                              ' «- « " 9^
                                                               ».i-:.'
                                                                        .j-^*
Fig.  15  Artificial  ground-water  enrichment scheme
  DOC
  m)l\


    10
    S
    I

   Q5
o Huhr water  (1 )•
n Preliminary filter outlet (H)
T Enriched ground water (5)
          I  fcfc J MarcJi I April  | Mqy [June I Jul^ I  *ug I  S«pt | OC1  | No»  | Dee
                                     "
Fig.  16   Elimination of  the DOC in artificial
           ground-water enrichment

-------
                         - 643 -
Fig. 17 gives the measured elimination of  the  Ruhr  zinc
content, which is on average 150  yg/1.  The measurements  were
made in the same system.  The mean elimination rate is  92%
(12).
  Inlet
    Zn
Elimination rate  90%  80%  50%
                  Remobi1izatioh
        <10 10
        50  100
500  1000/jg/IZn
     Outlet
 Fig.  17  Preliminary gravel filter, slow sand filter
          and soil passage(DFG 1/5)
 Fig.  18  illustrates the situation for copper, the mean con-
 tent  of  which in the raw water was 30 yg/1.  In this case the
 mean  elimination was 65%.  However, some analytical data
 clearly  lie in the right-hand ,field.  As the copper concen-
 tration  in the ground water was higher than that in the raw
 water, the possibility of remobilization processes must be
 considered.

-------
                          -  644  -
Inlet
       Elimination:    90V. 80'/.   50%
             Remobilization
   <2,5
                                           Pig. 18
                                           Preliminary gravel
                                           filter, slow  sand
                                           filter and soil
                                           passage(DFG 1/5)
               5   10
    50  100yug/lCu
        Outlet
 Fig.  19 shows the  same situation as  a function of time.   It
 can be seen clearly that the poor or even negative results
 again occur in the summer months, from which  it can be
 deduced with high  probability that algal excretion products
 are involved in these processes  (12).
   Cu
  jugl(
   100
   50
    10
    5

   <2.5
-°~ Ruhr water (l)
0  Preliminary filter outlet (4) __
~r~ Enriched ground water (5)
      Jon. |  Feb | Mdrz | April | Mai  | Juni | Juli  | Aug. | Sept. | Ok«.  | Nov. | Dez
                               1977
Fig.  19  Elimination of  copper in  the artificial enrichment
          of ground water  (DFG 1/4/5)
 Whereas we have been able to extend considerably our  knowledge
 of the activity of slow sand filters with respect  to  micro-
 impurities in  recent years, this  has given rise to many new
 problems that  require further elucidation.

-------
                        -  645—.
.(•!)• SCHMIDT, K.H.
    Die Abbauleistungen.der Bakterienflora bei  der  Langsam-
    sandfiltration und ihre Beeinflussung durch die Rohwasser-
    qualitat und andere Umwelteinf liisse. Biologische Studien
    zur kunstlichen Grundwas-seranreicherung
    Veroffentl. d.  Hydrol. Forschungsabt. der  Dortmunder
    Stadtwerke AG  (1963), Nr. 5"

(2) STUHLMANN, F.                                       '
    Studien zum Verhalten stickstoffhaltiger Wasserinhalts-
    stoffe bei der Langsamsandfiltration
    Verof f entl. des Instituts' fiir Wasserforschung GmbH
    Dortmund und der Hydrol. Abt. der Dortmunder Stadtwerke
    (1972), Nr. 10

(3) SCHOTTLER, U.
    Schwermetalle und Reinigungsleistung von Langsamsand-
    filtern
    Fachtagung der Deutschen .Sektion fur Limnologie in  der
    Internationalen Vereinigung  fiir Limnologie,  Siegburg
    6-10 Okt.  1975

(4) SCHOTTLER, U.
    Das Verhalten von Schwermetallen bei der Langsamsand-
    filtration            .'•-
    Zeitschrift der Deutschen Geologischen Gesellschaft
    126 (1975). , 373-384-   -

(5) ZULLEI, N.
    Polychlorbiphenyle '- Literatur - Analytik-  Langsam-
    sandfiltration
    Verof f entl. des Instituts fiir Wasserforschung GmbH
    Dortmund und der Hvdrol. Abt. der Dortmunder Stadtwerke AG
    (1977), Nr. 24    '         '

(6) BAUER,  U.             '':'.-•
    tiber das Verhalten von Bioziden bei der Wasseraufbereitung
    - unter besonderer Beriicksichtigung der Langsamsand-
    filtration           -  ", .     '
    Verof f entl. des Instituts fiir Wasserforschung GmbH  Dortmund
    und der Hydrol. Abt. der Dortmunder Stadtwerke  AG (1972)
    Nr. 15

(7) KLEIN,  G.              ,'
    Studie zum Verhalten von Algenabbauprodukten bei der
    Langsamsandfiltration       •
    (in preparation)

-------
                       - 646 -
 (8)  SCHC5TTLER,  U.         .
     Naturliche  Filtrationssysteme und Schwermetalle,
     aufgezeigt  am Beispiel von Quecksilber und Cadmium
     J.  Vom Wasser 49 (1977), 295-313

 (9)  KLEIN, G.
     Die Bildung' von Tryptophanmetaboliten beim anaeroben
     Abbau von Algen und deren Bedeutung fur Gewasser und
     Wassergiitewirtschaft
     Z.  f. Wasser- und Abwasserforschung 9_ (1976), 2, 55-59

(1O)  N&HLE, C.
     Zwischenbericht  1978 zum KfW-Forschungsvorhaben
     C.OO-4.O1/78 "Untersuchungen iiber den EinfluB biogener
     und anthropogener Komplexbildner auf natiirliche
     Filtrationssysteme" (1978)

(11)  SCHOTTLER,  U.
     AbschluBbericht zum DFG-Forschungsvorhaben Schm 294-12
     "Das Verhalten von Spurenmetallen bei der Wasserauf-
     bereitung unter besonderer Beriicksichtigung der
     kiinstlichen Grundwasseranreicherung"

(12)  SCHQTTLER,  U.
     Die Aufbereitung von schwermetalibelastetern Ober-
     flachenwasser durch Langsamsandfliter
     11. Essener Tagung, 8-1O Marz 1978 (in press)

-------
                        - 647 -
REMOVAL OF TRACE CONTAMINANTS FROM, RECLAIMED WATER
DURING AQUIFER PASSAGE   -

P.V. Roberts
In the arid Western regions of the U.S.A., reclamation
and reuse of wastewater is assuming an increasingly im-
portant role in planning to meet future water supply
needs. Potable reuse of highly treated reclaimed waters
is among the alternatives being considered. Potential  .
health risks posed by trace organic and inorganic micro-
pollutants as well as pathogens are difficult to evalu-
ate.

Because of these risk factors, public health officials
in California require that reclaimed water may not be
used directly as a potable supply but rather only by
the indirect route of groundwater recharge. Water for
potable reuse must be treated by granular activated car-
bon with an empty bed contact time of at least 3O mi-
nutes. The treated water must then be introduced into
the groundwater either by percolation from the surface
through the vadose zone or by direct injection into a
confined aquifer. It must be demonstrated that the re-
claimed water has resided in the groundwater zone for
a minimum of one year prior to extraction for potable
reuse.

In view of the public health risks entailed by potable
reuse, the authorities certainly are justified in their
cautious stand that requires groundwater passage prior
to potable reuse. Water quality benefits anticipated to
result from passage through an aquifer .include :

-------
                       -  648

(1) reducing the concentrations of contaminants, and
(2) damping concentration fluctuations so as to decrease
the frequency of extreme high values. Unfortunately, da-
ta are lacking to help us evaluate what degree of quali-
ty assurance is provided. Particularly for the organic
and inorganic micropollutants that are suspected of ha-
ving chronic toxic or carcinogenic .effects, little is
known regarding elimination or retention in aquifers.

This research was undertaken to answer questions con-
cerning the contribution of .aquifer passage to improv-
ing the reliability of a water reclamation system. In-
vestigations of the transformations and fates of trace
contaminants, especially organic micropollutants, are
emphasized. Specific objectives include the following:

     1.  To determine the extent to which trace conta-
        minants are removed.during aquifer passage.
     2.  To identify the processes responsible for re-
        moval, e.g. biodegradation, adsorption, ion
        exchange, chemical oxidation, or precipitation.
     3.  To quantify the rate of transport of trace con-
        taminants relative to the rate of movement of
        injected water.
     4.  To estimate the field retention capacity of
        the aquifer with respect to individual trace
        contaminants, where processes such as adsorp-
        tion or ion exchange are believed responsible
        for removal.

Experimental Methods

Reclamation Facility

The data presented herein are based in large part on
field studies carried out at the Palo Alto Reclamation

-------
                        -  649 „-
Facility. This facility consists of a water reclamation
plant and a well field for groiindwater recharge  (1). The
hydraulic capacity is O.09 m /s.
The water reclamation plant is an advanced treatment fa-
cility based on the concept of physical-chemical treat-
ment. The unit operations include high lime addition,
coagulation, and sedimentation; ammonia removal by sur-
face aeration; ozonation; granular activated-carbon
treatment; mixed-media filtration; and disinfection
with chlorine. The anticipated quality of influent and
effluent is summarized in Table 1.

During the observation period from which the data in
this paper are drawn, the reclamation plant was operated
in a start-up mode. Treatment consisted of lime treat-
ment at pH 9, air stripping, and'recarbonation, followed
by ozonation and sand filtration. The ozone dose was
approximately 50 mg/1. Owing "to the absence of activated-
carbon treatment, the organic* quality of the effluent
was not as good as anticipated for the full treatment
sequence. The average COD concentration in the effluent
was 20 mg/1.

The  full-scale  injection/extraction  well  field is  composed
of a network  of  9  injection wells, 9 extraction wells,  and
62 monitoring wells  in  an  area  approximately  3 kilometers
long by  1  kilometer  wide.  The wells  are intended for re-
charge and removal of water in  an aquifer  approximately
12 to  15  m below the ground surface  and 1  to  3 m thick.
Pilot Experiment

A pilot experiment was conducted at a test well from
August to November 1977 to gain experience with direct

-------
                         - 65O -
 TABLE 1
Projected quality of reclamation plant effluent
based on present Palo Alto secondary effluent
average characteristics and results of treatment
studies (2)

COD, mg/1
MBAS, mg/1
Nitrogen, mg/1 as N
NH3
N0~
NO3
Organic
Total
Heavy metals, yg/1
Cd
Zn
Cu
; Pe
i
! Mineral characteristics,
; rag/1
Sodium
Potassium
Calcium
Magnesium
Chloride
;
| Sulfate
Present -Palo
Alto Secondary
Effluent Average
Characteristics
• 53
0.12

24
O.3
0.4
"3."o
27.7

. - , ,2.5 ,
- 5
49
, 3
2.3

.". 1,62
11
43. , .
. -'15".
204 ' .-
85
Projected
Characteristics
of Reclamation
Plant Effluent
6
O.-O5

2-
0.3
0.4
0.5
2.7


-------
                         - 651 -
 injection of reclaimed water prior  to beginning  injec-
 tion and extraction at full scale.  The  test  site for the*
 pilot experiment is shown schematically in Figure 1.  Re-
 claimed water was injected into the recharge aquifer
 through Well 12. Wells P1 through P4 are sampling piezo-
 meters completed in the same aquifer, located approxima-
 tely 8 m from the injection well. From  core  samples  and
 drawdown test results, it was  concluded prior to the
 commencement of injection that the  injected  water would
 •flow preferentially in the direction of Well P4.  A
 drawdown test at Well 12 indicated  an average trans-
 miss ivity of .7 x 1O  m /m.s based on the assumption  of
 radial flow, but the transmissivity value in the direc-.
 tion-of P4 is believed to be substantially greater..  ..The .
 porosity of a core sample from Well P4  was 0.22.    i
      PI
                            S3
                   P4,
  P2
          X
      : :0.3m
>XP3
                             I.Om
                     2.1m
             .5m
    0.3m    12    ±O.3m
Fig. 1  .Injection and, observation well arrangement.for
      '  'pilot study.  12 is the injection well; P1, P2,
        P3, P4, and S3 are observation wells

-------
                        - 652
Analytical Methods

Highly volatile organic substances were analyzed using
a head space analysis technique  (3). Compounds deter-
mined by this method included halogenated aliphatics
containing one and two carbon atoms.

Moderately volatile compounds were determined using a
closed-loop stripping procedure  (4). Concentration of
the organic solutes was achieved by circulating air
for a period of two hours through a loop that passed
through the 5OO-ml sample and a  1-mg activated-carbon
adsorption trap. The adsorbed organic substance was
then eluted with carbon disulfide. The extracts were
separated by gas chromatography on a 20 m UCON HB510O
glass capillary column using a Carlo Erba gas chroma-
tograph with flame ionization detector. Quantification
was based on comparison with 1-Cl-Cg/ l-Cl-C.,-, and
1-Cl-C^g internal standard peaks. Identification of
peaks was achieved by means of a Finnigan Model 4000
gas chromatograph-mass spectrometer.

Trace metals were determined by flameless atomic absorp-
tion spectrophotometry (Perkin Elmer 403) following APDC
chelation and methyl isobutyl ketone extraction of un-
filtered, acid-preserved samples.
Interpreting Field Data

The experiment described is equivalent in principle to
imposing a step change in the composition of fluid at
the injection point. Prior to injection, the water in
the aquifer was relatively homogeneous. As injection

-------
                        -  653  -
proceeded, the formation groundwater was displaced out-
ward from the injection point toward and past the ob-
servation wells, resulting in concentration changes at
the sampling points.
Estimating the Rate of Transport and the Field Retention
Capacity

The rate of transport and field retention capacity for
a specific pollutant can be estimated from observations
of the concentration history at an observation point
following a step change in concentration at the injection
point  (5). The approach is premised on analogy to chemi-
cal reactor analysis  (6). The aquifer is treated as a
reactor of arbitrary shape and volume. The effective
pore volume of the aquifer element is evaluated from the
integral

           V  =  /°°(1 - fTT7)  dVTr7                   ...
            p   o        IW     IW                   (1 )

where V  is the effective pore volume in m , fiw the
fractional breakthrough of injection water as measured
by a conservative tracer, and VTW is the volume of water
             3
injected in m .  The field retention capacity of the aqui-
fer with respect to pollutant  i  is given by

                (  dviw
                  aq o

where r. is the specific retention capacity in g of com-
       1                 3          	
ponent  i  retained per m  aquifer;(C ).  is the average
                                               3
concentration of  i  in the injected water, g/m ; f.  is
the fractional breakthrough of component  i , a dimension-

-------
o' <1 - fl
o--" <1 - fi
w' dviw
> dviw
                       - 654 -
less function of the injected volume; and  e     is  the
                                           aq
effective porosity of the aquifer element. The  ratio of
the average transport velocity of a pollutant to that
of water is derived from a mass balance for the aquifer
element:
           ui   =	
          T]         "°°  I 1 — f \  JTT                    ( 3 )
           H2°
where UH „ is the average velocity across the aquifer
element Boundary in m/s ; and U. is the corresponding
velocity of pollutant i.
Identifying Transformation Processes from Field Data

The concentration response at an observation well can
be interpreted to identify the processes affecting the
transport of a pollutant. Several types of concentration
responses are illustrated in Figure 2, A conservative
tracer that does not interact within the aquifer will
appear as a sharp concentration front in the absence
of dispersion, or as an S-shaped wave if dispersion is
significant (6,7). A solute which is adsorbed in the
aquifer will be delayed compared to the conservative
tracer, and the length of the concentration front will
be extended. The concentrations of conservative as
well as sorbing solutes eventually reach the upper limit
imposed by the concentration in the injection water, at
which point breakthrough is complete. If a solute is
biodegradable under the conditions in the aquifer, its
concentration may first rise until a population of mi-
croorganisms has developed that is capable of metaboliz-
ing it after this acclimatization period; the concentra-
tion may then decline to a steady-state level, as shown
in Figure 2.

-------
                        - 655 -
     1.0
            EXPECTED RESPONSES TO A
         STEP CHANGE  IN  CONCENTRATION
   C.
   Co
      0
        PlugFbw'/DisPersion
 Adsorption
and Dispersion
                            J^qdegradation
                            and DisF>ersTo7T
       0123
        TIME RELATIVE TO MEAN  RESIDENCE
               TIME OF WATER, t/tH2o
Fig. 2  Forms of response to a step-change  concentration
        stimulus
 Hence, processes such as adsorption or biodegradation
 that may transform a solute or attenuate its movement
 can be identified by comparing the concentration res-
 ponse to that of a conservative tracer and to the ty-
 pical forms such as those in Figure 2.
  Results
 Breakthrough of Injected Water
  The rapid appearance of water of low salinity at Ob-
  servation Well P4 proved the existence of a good hy-
  draulic  connection between this well and the injection
  well,  confirming the expected preferential flow in the
  direction of Well P4 compared to other directions. Hence,
  monitoring  of  trace contaminants was limited to Well P4
  to the exclusion of the other observation wells.

-------
                           656 -
  The  breakthrough of injected water was calculated from
  conductivity measurements.  The correlation coefficient
  between conductivity and chloride was O.967 for 17 paired,
  values, which is significant at the 99.99-percent level.
  The breakthrough at the observation well commenced with-
  in the  first 1O m  injected volume, reached a fractional
  value of O.5O at 35 m  injected volume, and was virtu-
  ally complete after 20O m  had been injected (Figure 3).
  The effective pore volume of the aquifer element defined
  by Well P4 is 45 m , evaluated from the integral in Fi-
  gure 3.  The average residence time in the aquifer ele-
  ment is  approximately 12 hours, at an average injection
  rate of one liter per second.
             FRACTIONAL BREAKTHROUGH  OF
                    INJECTED WATER
cr
u.
                                -tr
                           2OO
                   f NAREA=/(l-fIW) dVIW = 45m3
                          O
              0       50    (00    150
              CUMULATIVE VOLUME INJECTED, VIW, m
200
 3
Fig. 3  Breakthrough of injected water at observation well
        P4

-------
                              - 657 -
  Concentrations of Organic Micropollutants

  The  injection water contained measurable amounts  of re-
  sidual organic micropollutants that were not removed in
  treatment.  An overview of the compounds regularly quan-
  tified in  the injection water at  concentrations exceeding
  1OO  ng/1 is presented  in Table 2.  Their large number may
  be explained by  the fact that the water did  not receive
  activated-carbon treatment  during this  period of  obser-
  vation..Subsequent observations after commencement of
  activated-carbon treatment  have shown that substantially
  lower  concentrations of organic micropollutants are at-
  tained compared  to the values reported  here.
TABLE 2
                COMPOUNDS REGULARLY QUANTIFIABLE IN INJECTION WATER
                             CRITERION: CIW>IOOng/.|
              CLOSED LOOP STRIPPING ANALYSIS
                                               HEAD SPACE ANALYSIS
      CHLORINATED AROMATIC COMPOUNDS
                                 Cl
                       "Cl
       CMLOROBENZENE
         Cl      Cl
1,2   1,3   1.4     1,3,4
D1CHLOROBENZENE TRICMUOROBENZENE
   ISOMERS
      AROMATIC HYDROCARBONS
           CH=CH2
         STYRENE
                  NAPHTHALENE
      ARYL AND ALKYL CYANIDES
           CN
                  ALSO: €5 TO Cg ALKYL CYANIDES
                       C.H,
                           • CN
       BENZONITRILE
TRIHALOMETHANE COMPOUNDS

 CHCI,     CHLOROFORM

 CMCIg Br    BROMOOICHLOfiOMETHAME

 CHCIBr2    DIBROMOCHLOROMETHANE

 CHBrj     BROMOFORM


OTHER CHLORINATED ALIPHATIC COMPOUNDS

 ClgC'CHCI   TRKHLOROETHYLENE

 Cl2C=eCI2   TETRACHLOROETHYLENE

 CljCCHj    1,1,1-TRICHLOROETHANE

-------
                          - ^658 -
 Aromatic Compounds
  Data  for  aromatic and substituted aromatic hydrocar-
  bons  are  summarized in Table 3. From gas chromatogra-
  phic  results  it is apparent that a small number of
  compounds predominate in the injection water (Figure 4).
  The sources of chlorinated benzene compounds are be-
  lieved  to be  industrial solvent wastes, while benzo-
  nitrile is thought to be present in electroplating
  wastes. Heptaldehyde and styrene may be products of
  ozonation and chlorination in the reclamation plant  (8),

  The concentrations of organic micropollutants analyzed
  by closed-loop stripping analysis were near or below
  their respective detection limits at the observation
  well  shortly  after injection began (Table 3). The con-
  centrations of some compounds rose appreciably during
  the course of the experiment, as exemplified by chloro-
  benzene,  styrene,  and benzonitrile. During the final
TABLE 3
   CONCENTRATIONS OF AROMATIC AND SUBSTITUTED AROMATIC MICROPOLLUTANTS






CHLOROBENZENE
1, 3-DICHLOROBENZENE
1,4-DICHLOROBENZENE
1, 2it>ICHLOROBENZENE
1, 2, 4-TRICHLOROBENZENE
NAPHTHALENE
STYRENE
HEPTALDEHYDE
BENZONITRILE
CONCENTRATIONS, ng/Jl
INJECTED WATER
ENTIRE PERIOD;
V = OT045OOm3
LOG
MEAN
4,130
630
95% CI FOR MEAN
(n = 9)
1,480 TO 1 I.5OO
255 TO 1,550
530 ' 265 TO 1 ,060
1,940
150
910
1,000
1 1,700
5,500
1,160 TO 3.25O
38 TO 590
320 TO 2,540
500 TO 2,000
9,500 TO I4.5OO
3,300 TO 8,900
OBSERVATION WELL
Vny = 0 TO 5OOm3

-------
                            659 -
  third of  the observations*  the  concentrations of chlo-

  robenzene and  styrene "were  not"significantly different

  from their respective -concentrations  in the injected

  water, as demonstrated  by the overlap of the 95 % con-

  fidence intervals  for the mean  (95  %  CI in Tpble 3).

  This is taken  as an  operating definition of "complete

  breakthrough."

A




16

12 9/15/77
2000 NG/L IS








15 II ' 8
1 ? '? f Is?

P4 INITIAL
B


15,

C

1

ra
—^~L^^

50 40 '
170° ISO" !3O°
200 NG/L IS


15 - - . -
P4 9/15/77 ', " '•'•
10CO NG/L IS. ' •




8" •






7-'


f

5




4A
(-80
-70
• 60
••50
-40
-30
• 20
- 10
H-Ut-x^ium. , Q
- ' ' 60
• 50
- 40
- 30
• 20
.- 60


5 . 8 '-

n •' -
.if . f llr»9 •

30 . 20
IIO° 9O° 70°


7
— «_
10

6
1

5




43o
ILjmcj
• 50
- 40
• 3C
. • 20

• 1C
^ .- 0
• 5 TIME ICT
50° TEMP ;°







LjJ
_ J
t )
_1
	 f
fc
a
E:
i






in)
C)
Fig. 4  Gas-chromatographic analyses of  injected water  and
        observation well samples

Substances identified are:  (1) toluene + tetrachloroethylene;
(2),  ethylbenzene;  (3) p-xylene;  (4) m-xylene;  (5)  chlorobenzene
with trace of o-xylene;  (6) unknown y;  (7)  sturene  +  unknown;
(8) ClC8  {Internal Standard)?  (9)  1,3-dichlorobenzene;
(1O) 1,4-dichlorobenzene;  (11) 1,2-dichlorobenzene;  (12)  benzo-
nitrilej  (13) 1,2,4-trichlorobenzene;  (14)  naphthalene;
nS) C1-G12  (Internal-Standard) ;. (16) C1-C116  (Internal Standard)

-------
                         - 660 -


  The concentration response of chlorobenzene is plotted
  in Figure 5 in a form suitable for estimating the
  field retention capacity and the relative transport
  velocity. According to Eq. 2, the field retention ca-
  pacity for chlorobenzene is
Co0/ro
                       dviwi    °-0041
                                       m
158o[m3]
     1
     "aq o
              - fiw)   dviw.
                              0.22
                                     45 [m3]
                             = O.032 g chlorobenzene re-
                               tained per m  aquifer
  •and the ratio of the transport velocity to that of wa-
  ter according to Eq. 3 is      '                   .   '
             u
              •C^H5C1
     ssL    C\ Id*.ii i I i i i i I i i i i I i i'i i I t i i i I i i i i 1 i i i 'I i r i i i i i i i i
     h     0      1000   2000    3000    4000
     tr
             CUMULATIVE -INJECTION VOLUME, m3
Fig. 5  Response of chlorobenzene 'concentration to a
        step-change stimulus

-------
                          - 661  -
  Halogehated Aliphatic 'Compounds
  Data for the components analyzed by head space analysis
  are shown in Table 4. During the early portion of the
  experiment  (VTW < 2OO m ) the concentrations at the ob-
  servation point were reduced to less than the detection
  limit for all components. Breakthrough was observed at
  a point corresponding to 1OOO m  injected volume or less.
  For all compounds, the 95 % confidence limits for con-
  centrations at the observation well during the period
                                          3           3
  embracing injected volume between 10OO m  and 15OG m
  overlap those for the injected water. Unfortunately the
  confidence  limits are quite broad, owing to the varia-
  bility of the analyses and the small number of samples.
  Only, the data for chloroform are sufficiently reliable
  to permit interpretation in the form of a breakthrough
  response  (Figure 6). It appears that the midpoint of
  the chloroform breakthrough is reached at approximately
  2OO m  injected volume. The breakthrough appears to be
  virtually complete by 1000 m  injected volume. The rate
TABLE 4
Concentrations of halogenated aliphatic
micropollutants •





CHLOROFORM
1,1,1-TRICHLOROETHANE
TRICHLORETHYLENE
BROMODICHLOROMETHANE
TETRACHLOROETHYLENE
DIBROMOCHLOROMETHANE
BROMOFORM
CONCENTRATIONS, fj.q/jl
INJECTED WATER
ViW=O-TO I50Om3
LOG'
MEAN
3.3
2.7
9.9
I.I
O.5
5.1
3.3
95% CI
FOR MEAN
'1.9 TO' 5.8
0.63 TO 11.5
4.i TO, 24 ,
O.3 TO 4.3
O.I2TO 2
2 TO 13
0.33 TO 33
OBSERVATION WELL
V1W=OTO 2OOm3
LOG
MEAN
<0.l
< 0. 1
< 0. 1

-------
                           - 662 -
   of transport  of chloroform can be  approximated only'
   very roughly.  The 'best estimate  of the ratio of the
   chloroform  transport velocity to the velocity of water
   movement is
             u
               CHC1.
                          45
             uwater   45 + 2O°      5
   evaluated from Eq.  3. The field  retention capacity, for
   chloroform  is
                  x 200 [m3]
0722  X  45  fm ^
                              =' O.OO32
                                         g chloroform retained
                                            m  aquifer
               BREAKTHROUGH-OF CHLOROFORM
                                         . o
                    AREA= /(fiw-fcHCI J dVlW = 200 m3
                         0       '        • '     .

                     0         	 INJECTED WATER
                              o	CHCI3
          .5      I.O      I.5      2.0
         CUMULATIVE VOLUME INJECTED, I03m3
                                               2.5
Fig. 6   Response of chloroform to a step-change  stimulus

-------
                          - -663 -'
  Biodegradation of Individual  Organic  Compounds         -•: M

  The behavior of naphthalene exhibited strong evidence .
  of the influence of biodegradation (Figure 7) ., The conj:
  centration rose significantly above the background value
  during the period following the  breakthrough of injected
  water. The concentration  exceeded 1OO ng/1 in the range
  between 40 m  and 15OO  m   Injected volume, exceeding 10
  percent of the average  injected  concentration through-
  out that range. A peak  concentration of 90O ng/1/ ap-
  proximately equal to  the  average injected concentration,
                                      '       3
  was reached at an injected volume.. of 680 m . After
  15OO m  injected volume,  the  concentration decreased
  below 1O percent-of the average  injected concentration
  for the remainder of'the  observations. This decrease
  cannot be explained by  a  secular decrease in the con-
  centration in the injected water.
                 NAPHTHALENE RESPONSE
                            o --
— INJECTED WATER
---NAPHTHALENE
                 1234
                CUMULATIVE VOLUME INJECTED, IO3m
Fig. 7  Response of -naphthalene

-------
                        - 664 - =
A plausible explanation for the decline in naphthalene
concentration at the observation well is that the rate
of biodegradation was enhanced by the development of a
population of microorganisms capable of metabolizing
styrene. It is hypothesized that acclimation occured
during the initial breakthrough of naphthalene. The
onset of biodegradation .after an initial lag period
is commonly observed in degradation studies. Further-
more, it has been postulated that substrates will be de-
graded in natural systems-'to the point at which a low,
steady-state concentration is reached (9). McCarty  (9)
estimated the steady-state concentrations of acetate
and glucose after an infinite residence time in an
                                 — 8
aerobic environment to be 25 x 1O   mol per liter and
       _ o
13 x 10   mol/liter, respectively. These conditions cor-
respond to the open ocean, or -large oligothrophic lakes.

The steady-state concentration of naphthalene observed
in the groundwater environment in this work is 0.47 x
  -9            -9
1O   + O.17 x 1O   mol per liter. Hence, the value for
naphthalene is two to three orders of magnitude lower
than expected for more readily degradable substrates
such as acetate and glucose. From this comparison it
can be hypothesized that the groundwater zone consti-
tutes an environment especially amenable to biodegra-
dation.                 '
It is possible that biodegradation also was responsible
for the removal of heptaldehyde. However, the field data
provide no confirming evidence in the form of a concen-
tration peak followed by a decline to a low, steady-
state concentration. Since heptaldehyde is believed to
be readily degradable, it is conceivable that the acclim-
atization of microorganisms occured within the time
frame of the breakthrough of injected water.

-------
                        - 665 -
 It .might be suspected that,the time of travel between
 the injection and observation points, i.e. approximately
 12 hours, was too short for biodegradation of poorly de-
 gradable substrates to occur. Concentrations of organic
 micropollutants were determined before and after a 25-
 day "rest period" during which no water was injected in-
 to the aquifer (Table 5). Since hydrogeologic studies
 showed no evidence of regional flow in the aquifer, it
 can.be assumed that essentially the same water was sam-
 pled at the beginning and end of. .the period. Of the
 eight compounds analyzed, the. concentrations of six
 appeared to decrease, while two-others increased. Be-
 cause only a single pair of measurements was made, no
 statistical conclusions can be. reached. It is believed
 that only changes by a factor- greater, than two can be
 considered significant. Only the .decrease in the con-
 centration of styrene, which amounts to a tenfold change,
 is thought to be significant. Biodegradation is a plau-
 sible explanation for the disappearance of styrene (1O).
TABLE 5
CONCENTRATION CHANGES OF ORGANIC MICROPOLLUTANTS
DURING A 25-DAY RESIDENCE PERIOD

CHLOROFORM
TRICHLOROETHANE
TRICHLOROETHYLENE
TETRACHLOROETHYLENE
CHLOROBENZENE
1, 2-DICHLOROBENZENE
STYRENE
NAPHTHALENE
CONCENTRATIONS, /ig/l
INITIAL
2.4
2.3
3.3
1. 8
2.4
0.38
2. t ,
0.035
AFTER
25 DAYS
2.7
2.2
3.I
I.I
• I-7
0.18
0.15
0.05
CHANGE,
PERCENT
+ 12
- 4
- 6
-40
-30
-47
-93
+ 40

-------
                       - 666 -
Chloroform, trichloroethylene, tetrachloroethylene, chlo-
robenzene, 1,2-dichlorobenzene, and naphthalene were not
degraded to a significant extent under the conditions of
this experiment. However, it must be borne in mind that
the conditions were anoxia, less than O.5 mg/1 dissol-
ved oxygen.
Moreover, the concentration of naphthalene at the samp-
ling point was very low, corresponding to the steady-
state level that presumably represents the lower limit
attainable by biodegradatipn. Hence, it may not be in-
ferred from these data that,, the1 substances in question
would not be degraded in other,situations in which their
concentrations were higher-and sufficient oxygen were
present.                 _   '-.•••• ;
Dissolved Oxygen  and Organic Collective Parameters

Dissolved oxygen, COD,  and „TOG were, determined  in paired
samples from  the  injection well head  and Well P4  (Table 6),
There was a consistent  decrease in  dissolved oxygen  con-
centration between  the  injection well and Well  P4. The in-
jected water  was  saturated with oxygen, having  an average
concentration of  8  mg/1. Samples from Well P4 consistently
showed an oxygen  concentration less than O.5 mg/1. The
disappearance of  dissolved oxygen coincided with a de-
crease in the COD concentration of  the same magnitude
 (Table 6) .                 '

From this agreement it  can be inferred that aerobic  de-
gradation of  organic substances occurred during the  pe-
riod of residence in the aquifer. The concentration  of
total organic carbon indicates that the biodegradation
must have been relatively complete. The ratio of the COD

-------
                         - 667 -
TABLE 6  DECREASE IN CONCENTRATIONS OF DISSOLVED OXYGEN AND
         COLLECTIVE ORGANIC PARAMETERS DURING AQUIFER PASSAGE

DISSOLVED OXYGEN, mq/Ji 02
COD, mg/J Og
TOO, mg/J C
(ACOD)/(ATOC)
DECREASE BETWEEN
INJECTION AND
OBSERVATION WELLS
MEAN
8.5
8.8
3.4
2.6
STD, DEV,
5.5
2.1


NUMBER
OF PAIRED
OBSERVATIONS
16
13


 decrease to that for TOC corresponds to the range of
 2.5 to 3.5 expected when organic substances present in
 wastewater are completely oxidized to end-products such
 as CO2 and H2O (11). However,  the data are insufficiently
 precise to permit a firm conclusion in this regard.

 It is unlikely that the observed reduction in the con-
 centrations of COD and TOC resulted from the removal of
 particulate organic material.  The turbidity of the in-
 jected water was less than 2 FTU.
 Removal of Trace Metals
 Concentrations of trace metals are summarized in Table 7.
 Ag and Cu were removed during aquifer passage throughout
 the observation period. Cd and Pb were removed during the
 early part of the period,  but concentrations at the ob-
 servation well were not significantly different from
 those in the injected water at the end of the period.
 The concentration of As was higher at the observation
 well than at injection point throughout the experiment, "
 possibly owing to dissolution or desorption of As from
     aquifer minerals.     •

-------
                         -  668 -
TABLE 7
    REMOVAL OF TRACE METALS DURING AQUIFER PASSAGE

Ag
As
Cd
Cu
Pb
CONCENTRATIONS./zg/l
INJECTED WATER,
MEAN ± STD. DEV
FOR ENTIRE PERIOD
2.0 ± 1.6
l.2± 0.7
2.0± 0.8
102 ± 24
1.3 ± 1.0
5 SAMPLES DURING
EARLY PART
OF PERIOD
V = OTO 500m3
INJECTED
MEAN
< 0.5
15
1.0
4.4
0.6
STD. DEV.
	
5.4
0.9
3.7
0.2
5 SAMPLES DURING
LATTER PART
OF PERIOD
V=3000T045OOm3
INJECTED
MEAN
< 0.5
12
1.6
28
1.6
STD. DEV.
—
2.5
1.7
3.2
0.7
 The breakthrough of Cd and Cu are compared to that for
 the injected water in Figure 8. The fractional break-
 through of Cu reached a value of approximately O.3 at
 the end of the experiment after 45OO m  had been injec-
 ted. Hence the rate of transport for Cu is estimated to
 be less than one-hundredth as great as the rate of move-
 ment of the water through the aquifer. The breakthrough
 of Cd is more rapid, the midpoint being reached within
 an injected volume of 15OO m .  The rate of Cd trans-
 port is estimated to be  approximately one-fortieth as
 rapid as that of water movement.
 Adsorption is the probable mechanism for trace metal re-
 moval in the aquifer.  The field retention capacity for
 Cd is calculated to be 17 mg Cd retained per m3 aquifer.
 Since the breakthrough of Cu was incomplete, we can only
 evaluate a lower limit for the field capacity, approxi-
 mately 2 g Cu per m  aquifer.

-------
                          -  669  -
              RESPONSE OF TRACE METALS
l.y
X
o

o
K
H 1 O
5 ''U
<
Ld
CC
CD
 XAREA=J(l-fCd)dVIW = l600m3
Ix O
^s o ___ja— o- — — '

VQ°'^ i i i i
                1234
              CUMULATIVE VOLUME INJECTED, I03m3
Fig. 8  Responses of Cd and Cu
 Conclusions
          Much  can be learned about the behavior of trace
          contaminants in the groundwater environment by
          conducting controlled stimulus-response experi-
          ments under realistic field conditions.
          From  the response at an aquifer sampling point
          following a step change in concentration at the
          injection point,  insights into water quality
          changes  can be obtained.  The processes respon-
          sible for removal can be  identified tentatively.
          The transport rate relative to water and the
          effective field capacity  can be estimated for
          compounds for which complete breakthrough is
          observed.

-------
                   - 670 -
3. Evidence of degradation in the aquifer environ- "•'
   ment was seen for naphthalene and possibly
   styrene. Naphthalene was degraded to a steady-
                                  — Q
   state concentration of O.5 x 1O   mol per liter
   following initial breakthrough.
4. Chlorinated aliphatic and aromatic compounds are
   retained effectively by adsorption during aquifer
   passage. Their concentrations initially are re-
   duced to less than O.1 yg per liter. However, the
   adsorption capacity ultimately is satured and
   breakthrough occurs.
5. The specific field retention capacity for chlo-
   robenzene was estimated to be O.032 g chloroben-
   zene per m  aquifer, at an average injected con-
   centration of 4 yg per liter. The specific re-
   tention capacity for chloroform was a factor of
   ten smaller, O.O032 g chloroform per m  aquifer
   at approximately the same injected concentration.
6. Organic micropollutants vary widely in the rate
   at which they are transported through an aquifer.
   The chlorinated aliphatic compounds, exemplified
   by chloroform, are transported most rapidly among
   the substances studied in this work. Chloroform
   was transported one-fifth as rapidly as the water
   with which it was injected. Chlorinated aromatic
   compounds are transported much less rapidly.
   Chlorobenzene moved 36 times more slowly than
   the injected water, for example. Dichloroben-
   zene and trichlorobenzene isomers are transpor-
   ted much more slowly.
7. Trace metals are removed in the aquifer by ad-
   sorption. Cd was transported most rapidly among
   the trace metals studied. Cd travelled with a ve-
   locity one-fortieth that of the injected water.
   The specific field retention capacity for Cd
                       3
   was O.O17 g Cd per m  aquifer.

-------
                        - 671 -


Acknowledgments

This work is funded by the U.S. Environmental Protection
Agency, Research Grant No. R-804431. Additional finan-
cial support was received from the Department of Water
Resources and the Water Resources Control Board, State
of California. The study is a cooperative venture with
the Santa Clara Valley Water District, who operate the
reclamation facility. The author is indebted to Dr.
Martin Reinhard and Joan Schreiner for the organic cha-
racterization data and to D. M. Mackay and G. D. Hopkins
for the trace metal determinations.

-------
                        - 672 -
 (1)  ROBERTS, P.V., MCCARTY, P.L., REINHARD, M., SCHREINER,
     Direct Injection of Reclaimed Water into an Aquifer
     J. Environ. Div. ASCE  (1978) (in press)

 (2)  MCCARTY, P.L., SCHERTENLEIB, R., NIKU, s.
f    Preproject Water Quality Evaluation for the Palo Alto
     Water Reclamation Facility
     -Technical Report No. 2O6, Civil Engineering Department,
     Stanford University, Stanford,  CA (1976)

 (3)  BELLAR,  T.A., LICHTENBERG, J.J.
     Determination of Volatile Organics at the pg/1 Level
     in Water by Gas Chromatography
     J. AWWA 6^7 (1974) , 634

 (4)  GROB, K., ZURCHER, F.
     Stripping of Trace Organic Substances from Water
     J. Chromatography 117  (1976), 285

 (5)  ROBERTS, P.V., McCARTY, P.L., REINHARD, M.
     Direct Injection of Reclaimed Water:  Attenuation of
     Organic Contaminant Movement
     J. WPCF, submitted for publication (1978)

 (6)  LEVENSPIEL, O.
     Chemical Reactor Engineering
     Wiley,  New York (1962)

 (7)  FRIED,  J.J.
     Groundwater Pollution
     Elsevier Scientific Publishing  Co.-, Amsterdam (1975)

 (8)  SIEVERS, R.E. et al.
     Generation of Volatile Organic  Compounds  from Non-
     volatile Precursors in Water by Treatment with Chlorine
     or Ozone
     Water Chlorination: Environmental Impact  and Health
     Effects, Ann Arbor Science Publ. Inc. Ann Arbor, Mich.
     £ (1978) , 615-624

 (9)  MCCARTY, P.L.
     Energetics of Organic Matter Degradation
     Water Pollution Microbiology, Wiley Interscience, Inc.,
     New York (1972), 5

(10)  SIELICKI, M., FOCHT, D.P., MARTIN, J.P.
     Microbial Transformations of Styrene  and  C   Styrene
     in Soil and Enrichment Cultures
     Appl. and Environm. Microbiology 35 (1978), 1, 124

(11)  WAGNER,  R.
     Abbaubarkeit und Persistenz
     Vom Wasser 4O (1973), 335

-------
                          - 673 -
BIOLOGICAL "PROCESSES FOR THE TREATMENT OF DRINKING WATER
J. Chedal
Following the lectures of this morning I should like to des-
cribe our experience with the biological elimination of
ammonia.

We tested the sludge-bed clarification plant described
by Dr.J.B.Goodall and found that this process has two dis-
advantages when applied to the raw waters used by us:

     fluctuating ammonia elimination rates

     when starting from an empty plant, a very long time
     until an active sludge layer is formed  (about 1 month).

The use of sand particles as in the Fluorapid plant definitely
makes it possible to eliminate the latter disadvantage to a
certain extent.  It should be added by way of explanation
that our experiments were performed with water containing
less than 2 ppm of ammonia.

It has also been established that the above-mentioned draw-
backs can be partially eliminated by increasing the ammonia
content in the v?ater.  However, we consider it ridiculous to
add ammonia to the water only to remove it again.  For this
reason we do not plan to incorporate a process of this kind.

As regards the biological filtration, the experiments so far
have shown that this plant operates much more satisfactorily
and delivers constant deposition of about 80% even in winter.

The main features of the plant are:

     increased water flow  (maximum velocity about 18 m/h)

-------
                          -• 674
-    air flow

     carrier layer enriched with trace impurities of the type
     of Biodamine.

As is well known, the filter is of the over-damming design,
in contrast to the bacteria beds in clarification plants.

In conclusion, reference may be made to the biological
activity of the rarely-mentioned storage process.

Our experiments have shown that with a residence time of 2
days this process makes it possible to eliminate about 50%
of the ammonia.

-------
                           - 6'75 -
NITROGEN REMOVAL IN BIOLOGICAL REACTORS AT LOW
TEMPERATURES

G. Halm0, K. Eimhjellen and T. Thorsen
Laboratory tests have been performed with continuous
biological reactors treating sewage and organic chemiT
cals at low temperatures. Three steps were operated in
series: Activated sludge, nitrification and denitrifi-
cation. Most effort was done to investigate the deni-
trification step and its performance.

The tests

Both activated-sludge and•denitrification steps have
shown better performance using psycrophilic or psycro-
trophic sludge if water temperatures are low. The  low-
temperature bacteria  have.significant effectiveness
right down to O C, optimum at .about 15 C and maximum
at about 20 C. Previous tests have shown that a deve-
loped low-temperature sludge has greater denitrification
velocity than high-temperature  (mesophilic) sludge
over a greater part of the low-temperature range.  It
has been realized, however, that around 17°C, an irre-
versible change destroys the good performance of low-
temperature operation.
Tests in a continuous laboratory plant,involving all
three steps, have been done at 5 ± 1°C. Due to high
COD reduction in the act, sludge step, methanol was
added to the denitrification step to ensure enough
feed for the bacteria. Between each change in operating
conditions, 1-4 weeks was allowed to attain pseudo-
equilibrium.                 •

-------
                           - 676; -
Results         ... .   .  ,    .        ...    ...„.,,,,

Because of unpredicted destruction of the Nitrobacter-
types in the nitrification  step, this step only
oxidized ammonia to nitrite during the later part of
the tests. However, this did not influence the effec-
tiveness of the denitrification step. Mean values
from several tests are shown in Table 1.
The results show that biological N-removal is easily
obtained at low temperatures. With the mentioned resi-
dence times approx. 9O% of  total N is removed in sewage
at 5 C. The nitrification/denitrification steps remove
approx. 95% of N from the act-sludge step. Denitrifica-
tion alone is more than 98% effective. Previous tests
indicate that about equal performance can be expected
at least down to 3°C. With  increasing temperature up to
about 17°C, the same performance may be attained with
shorter residence times.
TABLE  1  Mean values of  test  results  for  nitrogen  (ppm)
Influent
NH/
43
48
NO2+NO3
0.6
0.5
Res . time -*•
After acti-
vated sludge
NH4 +
27
34
N02+N03
2.2
0.6
1 .5 hours
After nitri-
fication
NH4 +
2.5
9
NO~+NO3
25
32
9 hours
After de-
nitrification
NH4
1 .5
8
NO2+N03
0.4
0.4
4 hours
 NB: First  line: Mainly NO., produced  in  nitrification  step;
     Second line: Mainly NO^ produced in nitrification step.

-------
                          - 677 -
Sedimentation of psycrophilic sludges is significantly"  '
better than corresponding mesophilic sludges. No bulking
problems were observed contrary to experiences' with
mesophilic sludges in the-same plant.
On-ly methanol was used as electron-donor and source of
carbon. Approx. 3 mg methanol is needed for each mg of N.
Microbiological investigations show that none of the
dominant bacteria are obligate methylotropes.
The results show that psycrophilic/psycrotrophic slud-
ges can be used with advantage for removing N at low
temperatures, particularly below 5 C. It seems that ni-
trification is the most sensible step, and further in-
vestigations should be performed here.
Even though these experiments-,were done with sewag'e and
chemicals, the results in general should be useful to
drinking water treatment. ,   .    -

-------
                          -  678  -
MICROBIOLOGICAL STUDIES ON ACTIVATED CARBON FILTRATION
P. Werner, M.Klotz and R. Schweisfurth


1.   Introduction
The results relate to the processing of Rhine water into
drinking water by means of activated carbon.

In regards to the method, the determination of the microorganism
count in the water poses no real problems if suitable nutrient
media, incubation temperature, and incubation time are used
after appropriate preliminary treatment.  Determination of the
colony counts in water according to the recommendations of the
Deutsche Einheitsverfahren is unsatisfactory, .since this method
only reflects a very small and non-representative part of the
microflora.  The reported colony counts were obtained by a
special method and are substantially higher than ones obtained
according to the Deutsche Einheitsverfahren.  Therefore, in
the light of these values, no statements on the hygienic and
bacteriological state of the water car be made in accordance
with the current Drinking Water Decree (Trinkwasserverordnung)[1]

2.   Quantitative determination of microorganism populations
When activated carbon is used in the treatment of drinking
water a bacterial population in the filtrate always occurs.
The colony counts in the characteristic treatment stages are
shown in Fig. 1.

As a rule colony counts of 2*10 /ml are found in the raw
water.  By -means of the preliminary chemical treatment of the
water the colony counts can be reduced to about 10 /ml before
the input to the activated carbon 'filter.  If this is followed
by a high-dose chlorination, nearly all the bacteria are
destroyed.  In the activated carbon filter an increase of the

-------
                          679 -
bacteria to values of about 7?10  /ml  again  occurs.  The acti-
                       %• x
vated carbon filter outflow is  normally  hygienically and
bacteriologically unimpeachable in accordance with  the current
Drinking Water Decree,
 10D-

 105-

 104-

 103~

 102~

 10* J
BAf
(KC
CTERIEN
WNIEZAHLlmlWASSBR) a)
•^n?-rt~" ."^T
1

PtlsgfSpl
¥ft§8
•

/ \
ROH-
WASSER
b)

""I'*5''I^^J"J^
^'
AKTIVKOF
E1NLAUF
OHhifMlf
CHLOR


HI
X*X* XvMvi
ti***t"r vXvX*.
ft***i*c **•*****%••>
Ulill
^iilll
r'x% ;¥:-X:K-'.-
•r-X'X'y.v:^:-:
Hpyiii
:?SS::xSr;
;:tv:::vi::::: •:'!'

\-^ ^.
HLEFILTER
lAUSLAUF
c)
\
 Fig.  1   Colony counts on SPC Agar at characteristic treatment
         stages in a Rhine water treatment plant

         Key;   a) = Bacteria (colony count/ml water)
                b) = Raw water
                c) = Activated carbon filter
                     inlet            outlet
                     with/without
                     chlorine

-------
                         - 680 -
Approximately a thousand times more bacteria per unit volume
can be found on the carbon than in, the water by colony count
determinations.

Since culture methods can never include all the live bacteria,
the living cell count is determined by enzymatic methods.
The total cell count  (all living and dead bacteria) was deter-
mined microscopically after enrichment on membrane filters
The colony count determination reflects up to 20% of all
living bacteria and up to 5% of the total cell count, i.e.
one including both living and dead bacteria.

3.   Qualitative determination of microorganism populations

3.1. Comparison of the populations in raw water and in the
     carbon filter
A comparison of populations with the methods of numerical
taxonomy should make it clear whether the bacterial flora
changes in the course of the water treatment,, while the
colony count remains practically unchanged.  The morphological
and biochemical properties of the bacterial strains were deter-
mined and later compared with the aid of a computer.

It was found that:
-    the adaptability of the bacteria in the activated carbon
     filter is lower than that of the bacteria in the raw
     water,
-    there are proportionally more bacteria of the Pseudomonas
     genus in the activated carbon filter than in the raw
                                                          j
     water ,
     the populations differ clearly in respect of the utili-
     zation of substrates, in particular harmful substances.

-------
                         - 681 -
A. special microorganism population thus develops  in the
activated carbon filter,  which is  different from  that of the
raw water, although both  populations have almost  the same
colony counts.

3.2.  The.  species composition of a microorganism population

3.2.1.. Bacteria    •    .  .  ' ,.  • :
A total of 26 species were isolated,  belonging to 11  genera
 (Table 1).   The majority of the bacteria belonged to  the
genus Pseudomonas.  From the point of view of species,  the
genera Bacillus and  Azomorias  were also well represented.
The bacteria found are 'not'pathogenic and are normally
present in water.
 Table 1   Bacterial  species  in the water of an activated
           carbon  filter
  Pseudomonas alcaligenes
  Pseudomonas cepacia
  Pseudomonas facilis
  Pseudomonas flourescens
  Pseudomonas lemoignei
  Pseudomonas mendocina
  Pseudomonas ruhlandii
  Pseudomonas stutzeri
  Pseudomonas spec..
  Gluconobacter oxidans
  Azoiaonas  agilis
  Azomonas  insignis
  Azomonas  macrocytogenes
Chromobacterium  violaceum
Neisseria sicca
Acinetobacter calcoaceticum
Micrococcus  luteus
Staphylococcus saprophyticus
Bacillus cereus
Bacillus circulans
Bacillus 'iicheniformis
Bacillus megaterium
Bacillus pumulis
Bacillus thuringensis
Corynebacterium  spec.
Micromonospora spec.

-------
                          - 682 -
3.2.2. Fungi

Moulds and yeasts are found seldom and irregularly in the
activated carbon filtrate.  They therefore play a'"sub-
 > r                                • ,     ,
ordinate role in the treatment of water.

Table 2 gives a summary of the moulds and yeasts which are..
found in the water of the activated carbon filters.   The fungi
present are non-pathogenic.

Table 2  Fungal species in the water of an
         activated carbon filter

 Phialophora hoffmannii
 Phialophora mutabilis
 Taphrina spec.

 Rhodotorula minuta var. texensis
 Cryptococcus  uniguttulatus
 Candida guilliermondii'var. guilliermondii
 Hansenula  anomala var. anomala

3.3. Bacterial capacity

Activated carbon adsorbs organic substances that can serve as
a substrate for the bacteria,  but it also adsorbs bacteria,
this adsorption following Freundlich's isotherm (Fig.  2).

Because of the large difference in s-izes, the bacteria and
the substrate are separated spatially  by the pore structure
of the activated carbon during the adsorption.   This has a
secondarily adverse effect on  the bacterial metabolism.   In
the absence of activated carbon the bacteria and the substrate
are distributed uniformly or enriched at the same points.

-------
                         - 683 -
Fig. 3 shows the metabolic activity of the bacteria as a
function of time.  The oxygen consumption serves as a measure
of metabolic activity.  Without activated carbon the degra-
dation of the substrate proceeds essentially more rapidly.
     Loading
      (logarithmic scale)
      [log  (colony count/g activated  carbon])
 10 —
  c __,	
     HhJ-

.JQO	 Loading
 80 -
 60 -
 40 -
 20 -
                       •10
                       10
             Adsorbed  concentration
              (log  [colony count/200 ml])
    o — o Q=32-c"~    a	o 0=10-c3--a
 Fig.  2   Studies  on  the  adsorption of bacteria on'activated
          carbon.   Isotherms

-------
                           - 684--
   SAUERSTQFFVERBRAUCH
   In*)   .
      a)
     I
                          ,-p©
     10
    c)
        20
 30
 d)
50
e)

                80
BAKTERIEN
1.2 10*/ml
1
2 	
S.UBSTRAT
PHENOL Ot1g/l


KOHLE
0,5g 1 -1.25mm


ZEIT (STUNDEN)
    b)
Pig. 3
Studies on  the effect of activated carbon  on  the
metabolic activity of bacteria
Utilization of phenol
         Key:
       a)
       b)
       c)
       d)
   = Oxygen  consumption (ml)
   = Time  (h)
   = Bacteria
                 e)  =
     Substrate
     Phenol,  0.1  g/1
     Carbon,
     0.5 g, 1-1.25 mm
Activated carbon also has a beneficial effect on the meta-
bolism of the  bacteria-.  It enriches organic  substances and
increases their  residence time in the filter;   a buffering
of the system  also occurs when toxic substances are present,

-------
                            - 685" -
Fig,  4  represents  the outcome of degradation experiments
with  four different concentrations of  phenol.
 15-
 10-
  5-
    SAUERSTOFFVERBRAUCH
     1 a)
        i—pi
        50
        r—j—r
        150
     c)
              200
          ZEIT (STUNDENi
                             b)
     BAKTEREN
     35 10s /ml
 SUBSTRAT PHENOL   KOHLE 0.5g
.2.5 a/I i...l.g/i 0.3g/l |0,1 g/l 1 -1.25 mm
 Fig. 4    Studies on the effect of. activated carbon  on the
           metabolic  activity of bacteria
           Utilization of various phenol concentrations.

           Key:  a)  = Oxygen consumption  (ml)
                 b)  = Time  (h)
                 c)  = Bacteria
                 d)  = Substrate
                       Phenol,  o.l g/1
                 e)  = Carbon 0,5 g,1-1.25  mm

-------
                         - 686 r , ,
At high phenol concentrations no degradation was found in the
absence of activated carbon, due to the toxicity of this
substance.  The following was found when activated carbon was
present: the carbon adsorbs the phenol, renders it "harmless",
and releases it continuously in the manner of a slow-flowing
carbon source in non—toxic concentrations.

3.3.1.  Contribution of the bacteria to the water treatment
The following values apply to the drinking water treatment of.
Rhine water with high chlorination before the activated carbon
filtration.  At the time of the studies the activated carbon
filter had an efficiency of about 80%.  The bacteria partici-
pated as follows in this treatment:

-       reduction of the amount of dissolved organic
        substances:  5%,
-       reduction of the amount of readily degradable
        organic substances  (BOD-):  about 70%,
-       reduction of the amount of difficultly degradable
        organic substances  (BOD^r*): about 17%
-       oxygen consumption:  about 60%,
—       carbon dioxide production:  about 60%.

It should be noted that during the subsequent inevitable
decrease in the adsorption capacity of the activated carbon
the bacterial fraction increases strongly in activity.  The
already low degradability of the organic substances in this
water is additionally reduced by the high chlorination.  The
high degree of the decrease of readily degradable organic
substances is significant as regards the repopulation with
bacteria.  These substances, causing the repopulation, are
partly removed by the biologically active activated carbon
filter and thus the tendency' towards bacterial population of
water in the supply network is reduced.

-------
                          - 687 -
In addition, it must be mentioned that the bacterial activity
effects a continuous partial regeneration of the carbon and
so prolongs its running time.

4.      Discussion and outlook
The occurrence of microorganisms and their proliferation in
the activated carbon filter was felt to be undesirable in the
past  - and sometimes still today  - without any exact know-'
ledge of the microbiological relationships and their signifi-
cance.

Accordingly, processes for disinfection of the activated
carbon filters were developed, which proved to be of no use
on a large industrial scale.

Bacteria on the activated carbon should not be combatted.  On
the contrary, their activity should be promoted, i.e. their
contribution, manifested ultimately in the conversion and
mineralization of organic substances, should be optimized by
suitable measures.  An example of this is offered by experi-
ments in which high chlorination  - which among its other
adverse effects impairs the degradability of the water consti-
tuents  - is replaced e.g. by ozonization to keep the readily
degradable compounds in the water.

-------
                      -' 688 -
(1)  KLOTZ,  M.,  WERNER,  P.,  SCHWEISFURTH,.R.
    Mikrobiologische Untersuchungen der Aktivkohle-
    filtration  zur Trinkwasseraufbereitung
    Forschung und Entwicklung in der Wasserwerkspraxis
    Wissenschaftl. Ber.ichte iiber Untersuchungen und
    Planungen der Stadtwerke Wiesbaden AG 3_ (1976),
    75-82

(2)  KLOTZ,  M,,  WERNER,  P.,  SCHWEISFURTH, R.
    Untersuchungen zur  Mikrobiologie der Aktiv-
    kohlefilter
    7.  Vortragsreihe mit Erfahrungsaustausch iiber
    spez. Fragen der Wassertechnologie
    Veroffentl. des Bereichs und des Lehrstuhls f.
    Wasserchemie der Universitat Karlsruhe (1975)
    9,  27O-282; English translation; EPA 6OO/9-76-O3O
    Dec.  (1976)

-------
                       -  689  -
PROCESSES DURING BIOLOGICAL OXIDATION IN FILTERS
D. van der Kooij
Introduction                    •          ; •

Biological processes, which result in the oxidation
of organic and some inorganic compounds as well as
in the removal of bacteria of hygienic significance
from water have always been important in drinking
water preparation. However, the increased pollution
of water resources by pathogenic micro-organisms and
non-biodegradable compounds forced the waterworks to
extend their water treatment systems by addition of
physicochemical processes including oxidation, ad-
sorption and disinfection. These techniques may have
direct effects on biological and physicochemical pro-
cesses, therefore selection and sequence of both bio-
logical and physicochemical processes are of major
importance to obtain optimal treatment efficiency.

Some recently introduced combinations of physioche-
mical and biological processes are: ozonation followed
by filtration and the use of granular activated carbon
(GAC) filters in which adsorption and biological oxi-
dation occur next to each other. This paper focusses
on some interactions between adsorption and biological
activity in GAC-filters applied to prepare drinking
water.

GAC-filtration proved to be very useful for the remo-
val of dissolved toxic or taste and odour affecting
organic substances originating from domestic and in-
dustrial water pollution. However, in GAC-filtrates

-------
                      - 690 -
frequently increased colony counts have been observed. .
(1/2,3,4,5'). This, increase of colony counts is in con-
trast with the reduction of bacterial numbers as usu-
ally observed in slow sand filters. Today it seems
widely accepted that the observed growth of micro-
organisms ,in GAC-columns .is enhanced by substrate en-
richment in the filter bed resulting from the adsorp-
tion of organic compounds by the carbon (6,7,8,5,9,
10,11).

Moreover, some investigators concluded that an in-
creased contact-time between organisms and substrate
(adsorbed) is allowing the organisms to adapt to the
less readily biodegradable organic substances. The
consumption of adsorbed compounds from the carbon has
been called "biological regeneration" of the carbon  (12)

The application of GAC-filters in drinking water pre-
paration in the Netherlands made it necessary to evalu-
ate the microbiological phenomena occurring in these
filters. Results of some experiments performed earlier
by the author (13)  revealed that die-off rates of some
types of micro-organisms on GAG and granular-non-acti-
vated carbon from filters supplied with river, water
during one year did not differ. These results sugges-
ted that -the micro-organisms were unable to utilize
organic substances adsorbed on AC.
Experiments

The influence of different filter materials on the
presence of micro-organisms in filter beds and fil-
trates was investigated using small experimental fil-
ter-columns containing GAG (Norit ROW 0.85), a non-
activated carbon (GNAC) with granules of similar di-
mensions and sand (diameter O.8-1.O mm). These filter-

-------
                       - 691 -
columns (diameter 6 cm) were supplied with tap water
(DOC: 3 ppm/ water temperature 14-18°C) without chlo-^
rine. The flow rate was 3.5 m/h and the apparent con-
tact time was 3 minutes. During a one-year period,
filter materials/ filtrates and the influent were sam-
pled and colony counts  (c.f.u./ml) were estimated.
For this purpose the surface spread technique on
8-fold diluted Lab-Lemco broth  (Oxord CM 15.) agar
plates was used. The samples of filter materials were
treated ultrasonically  for 3 minutes in the dilution
water to detach micro-organisms. The plates were in-
cubated at 25°C for 1O  days.
Moreover/ the removal of organic substances was mea-
sured by ultra-violet light absorbance at 275 nm in
5 cm cuvettes.
Results and discussion

The colonization of the filter: materials and the co-
lony counts of the tap water and the filtrates are
presented in Figure 1. Maximum colony counts on the
filter materials and  in the filtrates were reached
after a filtration period of 20 to 30 days. The ultra-
violet absorbences revealed that both the GNAC filter
and the Sand filter had an immediate total break-
through of UV-absorbing compounds. The GAC-filter
reached a breakthrough level of 8O % after 3O days.
After 5O days only 1O % of the UV-absorbing compounds
were removed by the AC-fliter.

A comparison of the colony counts observed on the
filter materials is presented in Figure 2. This fi-
gure reveals that the colony counts of GAC were usu-
ally larger than those of GNAC and Sand and reached

-------
                          - 692 -
                     A AC *—tfiltermaterial A---A water (effluent)
                     A NAC A—A  .   A—A
                     o Sand o—o  .   o—o
                                —• dnnkmgwater (influent)
       2O   4O  6O  BO,  1OO 120 14O  16O  18O  2OO  220 24O  26O  28O  3OO  32O  34
Fig. 1  Colony  counts of the influent, the  filter materials
        and  filtrates during a period of  34O  days

  a maximum  level  of  about 7x1O  c.f.u./ml. The  colony
  counts of  GNAC and  sand were similar to each other.
  The colony counts of the filtrates did  not  differ
  from each  other  (cf.  Figure 3).
  From the observed  similarities in microbial behaviour
  in the presence  of the three different filter  materi-
  als it is concluded that adsorption of organic com-
  pounds by the  activated carbon is not the  cause of
  the high colony  counts as usually observed in  GAG-fil-
  ters . This conclusion is supported by the  observation
  that the majority  of micro-organisms isolated  from
  GAG was only able  to grow  on simple non-adsorbing
  compounds like acetate, pyruvate and lactate,  whereas
  adsorbing substances like aromatic compounds were not
  utilized.

-------
                         - 693 -
    10
    10
  co
  L_
  o>
  •*-•
  CO
  E
  e  6
  •"-. 10
  3
  H-^
  cj
    10
      0    20   40    60    80
        —*" cum % of samples
100
Fig. 2  Comparison of the colony counts  of  the  filter
        materials GAG, GNAC and sand
  Observations on sand samples from  the  effluent side
  of the slow sand filters of -the Hague  (flow rate :
  O.3 m/hr) revealed that the bacterial  content  of
                             4
  this sand was about 2-3x1O -c.f.u, per ml,  whereas
  colony counts in the filtrate usually  were  below
  10O c.f.u. per ml. Similar observations were repor-
  ted by Schmidt (14). Comparison of these  colony
  counts with those observed in the  experiment (cf.
  Figures 2 and 3)  suggested that a  relationship may
  exist between the flow.-rate-.and the number  of-  micro-

-------
                         - 694 -
   c
   CD
   3
   
-------
                         - 695 -
  c f u /tnl GAC
    10'
     10*
     105
       O
20
r  i
  40
           60
cum. % of samples
80
                                    100
gig. 4  The colony counts of GAC present on  the  slow
        sand filters of The Hague
  The results presented  in  Figure  2  revealed that the
  colony counts of GAC were usually  larger than those
  of GNAC and Sand although the  colony  counts in the
  filtrates did not differ.  This phenomenon is probably
  due to the relatively  large  surface area per. unit vo-
  lume of the GAC, on which micro-organisms utilizing
  substrates from the passing  water  can attach. For this
  reason GAC may be a favourable material for biological
  filtration processes.

-------
                       - 696
Effect of micro-organisms on adsorption

In the previous part of this paper  it is  showji  that
colony counts on GAG, in filter beds may  reach  a maxi-
mum level of about 7x1O  c.f.u. per ml GAG  (i.e.
    P
2'. 1O  c.f.u, /gram GAG). Large numbers of micro-orga-
nisms on GAG in filters were also observed by Klotz
et al. (3). These large numbers of micro-organisms on
the GAG might hinder the, transport  of organic com-
pounds to the adsorbing surface of  the carbon.  Some
laboratory experiments were conducted to  investigate
the influence of bacterial ce,ll attached  on  GAG on the
adsorption of some compounds.. In these experiments,
adsorption isotherms and .adsorption rates of 4-nitro-
phenol and 4-hydroxybenzoate on GAG  (Norit ROW 0.-8
Supra) were estimated in the presence and in the ab-
sence of a large number of bacteria on the carbon.
The used bacteria were a P s eudomonas fluorescens
strain and a Pseudomonas alcaligenes strain. The
fluorescent pseudomonad could grow  on 4-hydroxyben-
zoate but did not utilize 4-nitrophenol,  whereas
P. alcaligenes could not use either of these com-
pounds. Portions of O.1 gram GAG were sterilized in
bottles containing 2OO ml demineralized water with
KH2PO4 2.7 mg/1; K2HPO4-3H20 .5.3. mg/1 and Na2HP04.
12H2O 8.O mg/1. Final pH after .sterilization: 7.3,
In order to cultivate bacteria on . the carbon ammo-
nium-acetate was added to the sterilized  bottles
from a separately sterilized solution in  a final con-
centration of 10 mg acetate-carbon  per liter. Further,
the bottles were inoceulated with either  the fluore-
scent pseudomonad or the P^^alca^lig^enes, isolate  and
incubated for 3 days at 25° _in a rotary shaker
(12O rev/min.). The bacteria developed to the maxi-
mum level of 4x1O  c.f.u. per ml of medium and  the
                       "A                    R
GAG contained about 2.1O  c.f.u. per ml  (6x1O /gr) of"

-------
                       - 697 -r-. -
carbon. In these conditions 4-nitrophenol was added
to the bottles with P. flucrescens and 4-hydroxyben-
zoate was added to the bottles with P.alcaligenes
from sterilized solutions in final concentrations
of 1OO mg/1; 5O mg/1;. 25 mg/1 and 10  mg/1. These com-
pounds were also added to bottles containing steri-
lized 'GAG without bacteria. The bottles were re-
placed in the rotary  incubator -at 25°C and the  con-
centrations of either 4-hydr<5xybenzoate or 4-nitro-
phenol were measured by UV-absorbance  (at 245 and
269 nm respectively)  in membrane filtered samples  of
5 ml after 24,48 and  144 hours of incubation. UV-
absorbances were measured every-hour  during the first
eight hours after addition in bottles with an initial
adsorbate concentration of 1OO'mg/l.  All experiments
were performed in duplicate.
Results and discussion

With both  compounds,  the  adsorption-equilibrium was
reached within  48-144 hours. The  adsorption-isotherms
of  4-nitrophenol and  4-hydroxybenzoate  on  the  carbon  in
the presence and _in  the abscence  of  bacteria  were  cal-
culated from measured concentrations and  are  presented
in  Figure  5. The disappearance  of the  adsorbates from
the solution initially containing 1OO  mg  of  adsorbate
per liter  is shown in Figures  6 and  7.  Figures  5,  6
and 7 show that 4-nitrophenol 'is better adsorbed than
4-hydroxybenzoate. The results  presented  in  Figure 5
reveal that the adsorption  isotherms were not affected
by  the described presence -of -:the  bacteria. Moreover,
the adsorption  of the adsorbates  in  the 100  ppm-bottles
was not affected by  the presence" df  bacteria  on the
carbon. The adsorption rate of- 4-hydroxybenzoate in

-------
                          -  698 -
                                            A AC without bactenx


                                            J AC with
02    04    06    08    tO    12    1.4    16    18
  -02
Fig. 5   The adsorption isotherms  (144 hours) of  4-nitrophenol
         and 4-hydroxybenzoate on  GAC (Norit ROW  0.8 Supra)
         in the presence and in the  absence of bacteria on the
         carbon
30
so
10
       &) AC witliuul bk«m


       I) AC with bjcleiu
Fig. 6

The disappearance of
4-nitrophenol and
4-hydroxybenzoate from a
solution  as a result of
adsorption on GAC with
and without bacteria
             TinwChoura)

-------
                        - 699 -
                  £) AC without tucteru
                  • AC with bacteria
                                        Fig.  7
the presence of bacteria could not be calculated be-
cause both bottles became infected by adsorbate-consu-
ming micro-organisms. This compound is,  in contrast
to 4-nitrophenol,easily biodegradable.

The experiments described in this paper  showed  that
the number of micro-organisms on a filter material
depends on the flow rate of the water through the fil-
ter bed. This phenomenon is probably due to  limita-
tion of substrate transport to the micro-organisms.
Moreover, it is shown that adsorption of compounds
was not inhibited by the presence of a large number
of bacteria on the carbon. GAG in filter beds sampled
by the author always had lower colony counts than the
level applied in the experiments described in this
paper. Therefore it is concluded that in most GAG—fil-
ters used to prepare drinking water, hinderance to

-------
                          - 7OO -
   the adsorption of organic compounds by micro-organisms
   is  very  unlikely. However, it.still may occur when the
   water  is containing relatively large amounts of bio-
   degradable  compounds in which situations extremely -  .

   large  numbers of micro-organisms develop on the carbon.
   Moreover, adsorption may be affected by the pollution

   of  GAC by colloidal and suspended matter. These effects
   were not investigated.
(1)   FORD, D.B.
     The use of granular carbon filtration for taste and
     odour control
     Proc. Wat. Res. Ass. Conf. Reading  (1973), 263-278

(2)   KNOPPERT, P.L., ROOK, J.J.
     Treatment of river Rhine water with activated carbon
     Proc. Wat. Res. Ass. Conf. Reading  (1973), 1O9-125

(3)   KLOTZ, M., WERNER, P., SCHWEISFURTH, R.
     Investigations concerning the microbiology of activated
     carbon f i1te rs
     Trans. Rep. Special Problems Water Techno1. EPA-6OO
     19-76-030 (1976), 312-33O

(4)   MELBOURNE, J.D., MILLER, D.G.
     The treatment of river Trent water using granular activated
     carbon beds
     Proc. Wat. Res. Ass. Conf. Reading  (1973), 73-1O8

(5)   WALLIS, G., STAGG, C.H., MELNICK, J.L.
     The hazards of incorporating charcoal filters into
     domestic water systems
     Water Research 8_ (1974), 111-113

(6)   WEBER, W.J., FRIEDMAN, L.D., BLOOM, R.
     Biologically extended physicochemical treatment
     Adv. Wat, Poll. Res., Proc. 6th Internat. Conf.
     Jerusalem (1972), 641-656

(7)   KOLLE, W., SONTHEIMER, H.
     Experience with activated carbon in West Germany
     Proc. Wat. Res. Ass. Conf. Reading  (1973), 347-367

(8)   LOVE, O.T., ROBECK, G.G., SYMONS, J.M., BUELOW, R.W.
     Proc. Wat. Res. Ass. Conf. Reading  (1973), 279-312

-------
                          - 701
 (9)   EBERHARDT,  M.
      Untersuehungen zur optimalen Kombination von Adsorption,
      Filtration  und biologischer Reinigung
      Publication Wasserchem.,  Engler-Bunte-Inst., Univ.
      Karlsruhe _5 (1971) , 358-279

(1O)   GUIRGUIS, W.,  COOPER,  T., HARRIS,  J./UNGAR, A.
      Improved performance of  activated  carbon by pre-
      ozonation
      J.  Wat.  Poll.  Contr. Fed. 5O (1978),  3O8-32O


(11)   SYMONS,  J.M.
      Interim  treatment guide  for controlling organic contami-
      nants in drinking water  using granular activated carbon
      US  EPA,  Cincinnati (1978)

(12)   EBERHARDT,  M.
      Experience  with the use  of biologically effective acti-
      vated carbon
      Trans. Rep. Special Problems Wat.  Technol.
      EPA-6OO/9-76-03O (1976).  331-347

(13)   Van der  KOOIJ, D.
      Some investigations into the presence and behaviour
      of  bacteria in activated carbon filters
      Transl.  Rep. Special Problems Wat. Technol.  EPA-6OO/9-
      76-030 (1976) , 348-354

(14)   SCHMIDT, K.
      Die Abbauleistungen der  Bakterienflora bei der Langsam-
      sandfiltration und ihre  Beeinflussung durch die Rohwasser-
      qualitat und andere Urawelteinflilsse
      Publication Hydrol. Fcrsch. Dortmund, Dissertationsschr.
      (1963) ,  5

-------
                         -  702  -
PROCESS ENGINEERING ASPECTS IN THE COMBINATION OF CHEMICAL
AND BIOLOGICAL OXIDATION
H. Sontheimer
Biological processes for removing dissolved organic substances
from water can only be applied successfully in the treatment
of drinking water if the substances to be removed are bio-
logically degradable in the time set by the selected process
and under the prevailing ecological conditions.  Furthermore,
the water must not contain materials that are harmful to
microorganisms.  As a result of these restrictions, but some-
times also for purely technical reasons, other purification
measures must first be introduced before the biological treat-
ment process can be run reliably and effectively.         ;

Among the most important of these are the processes that
allow a far-reaching removal of solids and colloids, i.e.
flocculation, sedimentation, and filtration.  These are per-
formed mainly to relieve the loading on the filter surfaces
in the biological filters and. to prevent them from blocking
up.  Measures of this kind are especially .necessary where
the access to the infiltration surfaces for the purposes of
cleaning is limited.  For this reason particularly high
demands are made in the case of underground seepage galleries
and absorption wells.  A preliminary treatment is here almost
invariably necessary.

If a flocculating /agent is added in such treatment processes
preceding t$.he biological purification, in many waters an
    ''i           '
additional removal of the dissolved organic substances takes
place, so genuinely, relieving the biological stage.  Fig. 1
shows, in the light of the results obtained at Gelsenwasser
AG, that a preliminary purification of this kind also has a
beneficial effect on the water quality after the soil pass-
age.

-------
                         - 7O3 -
Frequency suras in %
— ui ih '~t in ID
M eg ca ra ea - e S
1 I 111! 1 1

Ground water from 7
flocculated infil- /
trate f
*a 13
o o ,a
/Ground / /
^ater from /
o _.' w' /a
/ unf locou- /
ul a ted J «/
infUtPPte /
Cl/ , O
o/" X°
~V
£9 t3 Cl y/ O
n/ / Q/Ruhr water/ Ruhr water
T f /"after flo-/ before floccu-
/ / /cculation lation
2 3 4 5 6 7 8 9 10 .13
UV-absorption coefficient (254 ran) m
Fig.  1  Improvement of the quality of ground water by_
       infiltration of flocculated water at the Witten
       waterworks
This figure shows the frequency distributions for organic
substances determined by W extinction before and after
flocculation.  The results indicate that the decrease in the
organic loading caused by precipitation with the flocculating
agent still persists after the soil"passage.
A very similar result as regards the final outcome can be
achieved with a process of a very different kind, namely with
the aid of chemical'oxidation upstream of the biological
purification stage.  Although chemical oxidation on its own
is generally insufficient to reduce the total concentration
of dissolved organic carbon, but only the chemical oxygen
demand, this effect shows, just like the clear reduction of
the UV extinction when ozone is used, that when a chemical
pre-oxidation of this kind is performed a conversion of the

-------
                           -  7O4  -
 organic water constituents takes place.   In addition to the
 reduction of the mean molecular weight already mentioned in
 other  papers, particularly with ozonization,  this pre-
 oxidation also causes a rise in polarity  by increasing the
 proportion of the carboxyl groups and thereby a simultaneous
 partial conversion of biologically resistant organic sub-
 stances into biologically degradable ones (1,2).

 An example of this effect, which is representative for many
 similar observations, is shown in Fig. 2.
     14
     12
   -10
   la
Inflow
Without
ozone
With
ozone
      Ozone consumption:3"*"
     ni   iii   i    i
                                    Inflow

                                    [Without
                                    ozone
                                    With
                                    orone
                  8  10  12  14  16  18 20
                           weeks
Fig. 2  Influence  of  ozonization on the change in the DOC
        and COD  in the slow filtration of a biologically
        pre-purified  waste-water

-------
                         - 705' -
Here are shown the results for the further purification of a
Berlin waste water that has already been very effectively
biologically purified  (3).  It can be seen that by the treat-
ment with ozone in combination with biological oxidation in
a sand filter a clear  improvement in both the'DOC and the COD
may be attained.

In connection with the theme of the present paper there is a
finding  -  also shown  in  Fig.  2  -  that  is particularly
important.   It  is that the favourable effects of preliminary
ozonization can be easily detected  when  only  5.4 mg/1 of
ozone  has been  used.   No  further  detectable improvements are
obtained by the use of higher  doses.  'All the experience
gained so far with very different waters shows that on the
whole  only  about 0.5 g/g  DOC is necessary for such prelimin-
ary oxidizing treatment with ozone, and  that  the ozone
requirement never exceeds 1 g/g DOC.  This applies to crude
water  of normal composition, such as is  used  to obtain
drinking water.                          ...

When using  slow filters or soil infiltration  large surfaces
or suitable underground conditions  are normally necessary.
Since  these are not always available, much time has been
spent  on the possibility  of performing biological purifi-
cations in  filters as  well, and in  this  content biologically
and adsorptively working  activated  carbon filters have
aquired particular significance in  recent years.  -The bio-
logical processes that occur here have long been observed
but, owing  to the high bacterial  counts  found in many cases,
they have often been regarded  as  merely  disadvantageous.

The increased interest in chemical  and biological oxidation
with the use of activated carbon  filters in recent years is
partly due  to the results of studies carried  out initially
at the municipal works in Bremen  (4,5) and of subsequent

-------
                           - 706 -'
  studies both at an experimental plant and at a main'plant 'in
  Mulheim (6).  Details of the experiments and experience in
  Mulheim will be given in the next lecture.  Right now, how-
  ever,  using fairly simple arguments and model considerations,
  we  shall attempt to arrive at some conclusions about what
  can be achieved with a process of this kind, about its limit-
  ations, and how the observations made at various plants can
  be  explained.
  c
  Co
  t
  t
Fig.
                                25    30
                               t in weeks—••
                10
                      15
                      20    25    30
                       t in weeks —»
_3  Filter breakthrough curves  with (-«-•-)  and
   without  (	)  biological  reactivation
 We start out  from  the  premise  that for  a given water and a
 given activated  carbon filter  the  loading will increase
 steadily with time in  the case of  pure  adsorption,  as can be
 seen from  curve  1  in the  lower half of  the figure.   If the
 organic substances are removed by  adsorption processes alone,
 then, depending  on the nature  of the organic materials and
 on the quality of  the  carbon,  to each given loading in the

-------
                        - 707
filter 'there will correspond a certain outflow concentration
or a certain filter efficiency.  The higher the loading, the.
greater is the outflow concentration, as can be seen on the
example of curve 1 in the top half of the figure.

It has been shown repeatedly that biological processes can
lead to a reduction of the loading, since by means of these
the adsorbed materials are then.oxidized to CO- and H20.  If
a certain biological activity is assumed in the filter, we
obtain curve 2 for the time variation of the loading in
curve 1, i.e. with a biologically operating filter the load-
ing increases more slowly than with pure activated carbon.

On the strongly simplifying assumption that the relationship
between the loading and -the filter efficiency remains cons-
tant, the breakthrough curve 2 can be easily obtained from
the two loading curves and the filter activity curve 1, as
is shown here as an example, if an additional biological
regeneration of the activated carbon takes place.  The
better this regeneration, the,longer is the service life of
the filter until a certain residual concentration has been
reached. .In the simple example of a filter with a short
residence time shown in Fig. -3 the running time is prolonged
by about 50%.  In practice the corresponding values are
usually considerably higher.  A prolongation of the running
time by a factor of 3 - 5 is frequently observed, this
factor being decisively dependent on the residence time of
the water in the filter and on the proportion of biologically
degradable substances.

As shown in the next figure, it is also of significance for
prolonging the running time whether an appreciable part of
the adsorbed substances is biologically degradable.  In the
case of a high proportion of resistant materials these con-
centrate on the carbon in the course of time.  The biological

-------
                          - 708 -
                            20     25    30
                            t in weeks —^
                        without biological
                        reactivation
                        small proportion of
                        difficultly degra-
                        dable substances
                        large prooortinn of
                        difficultly degra-
                       tdable
                      IS
  20     25    30
t in weeks—*
Pig. 4  Breakthrough and loading courses for biological
—	  activated carbon reactivation
 activity must therefore gradually  decrease,  since smaller
 amounts of degradable materials are  present on the carbon,
 giving rise to a considerably earlier breakthrough
 represented by curve 3  -  in place of the improved break-
 through curve 2.
Among other reasons, this loading  with biologically resistant
substances becomes evident from the  fact that the oxygen con-
sumption for the biological; oxidation  decreases with increas-
ing  loading.

This  is  shown in the following figure,  based on results
obtained by Engels at the Dlisseldorf municipal works.

-------
                         - 7O9 -
  o
  Cr>
 220
 200
 180
 160
 140
 120
 100
 80
, 60
 40
 20
  0
            2000    40OO   6000   8000   10000  12000  1400O
                 Water  throughput in m3/m3 GAC
Fig. 5  Oxygen consumption ,in -GAG. filters, as a function
        of the water throughput
This unfavourable effect of  the  proportion  of difficultly
degradable  substances  explains why  much time and effort is
spent on reducing this proportion by  treatment with ozone.
A preliminary oxidation of this  -kind  is also carried out in
Dusseldorf, but  it  is  apparently- insufficient to prevent the
decreased oxygen consumption.
An example of  the  effects  that  can  in principle be expected
from a preliminary oxidation  in the breakthrough curves is
shown in Fig.  6.       ,  ,   -   ,  •
According to this,  when  a  small  amount of  ozone has been
added a clearly more  favourable  breakthrough  curve is  obtained,
since a part of the resistant organic material  becomes bio-
logically degradable  as  a  result of ozonization, so that the
previously mentioned  enrichment of  the difficultly degradable
materials on carbon does not occur  to the  same  extent  as in
the absence of such preliminary  treatment.

-------
                          - 710 -
                                                3mg/l03

                                              \6mg/I 0
Fig. 6  Example of the changes in  filter breakthrough curves
        with different ozone doses
Nevertheless, ozone oxidation, particularly when carried out
with excessive ozone doses, can also modify the adsorb-
ability of the organic  substances.  Thus, the more  strongly
polar substances produced by ozonization are often  adsorbed
less well.  In extreme  cases, as can be seen from the curve
in Fig. 5 for an addition of 6 mg 03/1, this effect can
cause the initial breakthrough to occur at the same time,or
under unfavourable conditions even earlier, than is the case
when no ozone has been  added at all.  Only with very long
running times and higher residual concentrations does this
method become advantageous again with high ozone doses.

Readily degradable organic compounds are often produced, when
high doses are used in  this way, such as acetic and oxalic
acid (7).  These are immediately biologically oxidized on
the outer surface of the•activated carbon and are thereby
rapidly and extensively removed.  Under such conditions the
breakthrough curves obtained basically follow the curve for

-------
                          -71.1 -
1 mg/1 of ozone, and later pass into one of the. curv.es  for  a
higher ozone dose.  Although the filter activity  is enhanced
under such conditions for all ozone doses, excessive doses
of ozone are undesirable from the economic point  of view.

This model explanation and clarification of the possible
relationships shows why in practice very different effects
are observed in dependence on toe water quality, the filter
dimensions, the properties of the carbon, and the amount of
ozone used.  For this reason the discussed combination  of
chemical and biological oxidation is not a treatment that
can be performed universally arid on all "waters with equal
effect and always following the same procedures.  Moreover,
since the possible dependences can seldom be obtained from
simple laboratory tests, the performance of semi-technical
trials is nearly always necessary.

If a summary of the essential criteria that 'can be deduced^
from the existing experience is nevertheless to be attempted,
despite the above' mentioned complexity of the situation in
"this oxidation process, 'the values given in the following
table are obtained.
 TABLE 1;  Process parameters for biologically adsorptive
           treatment of drinking water   .      ;•:,••.-
 Ozone dose
 Biological oxidation
 b~ demand
  (for DOC oxidation)
 Residence time in  filter
 presumed empty
O..5 - 1 .O g O3/g DOC
              3
   100 g -DOC/m"
   20O g' 02/m3
   15-30 min
d

-------
                         - 712 -
Accordingly, 0.5 - 1 g of ozone is generally required per g
DOC.  Larger amounts are almost invariably disadvantageous if
a different effect is to be avoided with the ozone.  In
nearly all of the filters studied so far an increase in the
amount of CO^ was found in the filter outflow under these
conditions, corresponding to a DOC of 100 g per m  of filter
volume and per day.  As a rule, 200 g of oxygen per m  of
the filter volume per day are necessary for this oxidation
of organic substances.  If NH, ox'idation is taking place in
the carbon filter at the same time the oxygen consumption
becomes considerably higher, but the C02 production is there-
by usually only slightly influenced.

The length of the residence time in the filter normally
determines the time between two regenerations.  If it is so
long that, calculated on the carbon, the incoming organic
substance corresponds in amount to the biologically oxidized
substance, a biological filter can theoretically remain in
use indefinitely, needing only a back-wash from time to time.

However, in practice the effectiveness of biological purifi-
cation nearly always deteriorates as a result of the accumu-
lation of resistant materials on the carbon surface.  Toxic
water constituents and their accumulation can also have an
adverse effect.  For this reason the running time of bio-
logical activated carbon filters is nearly always only some
5-10 times as long as that in the case of pure adsorption.
The prolongation factor decreases with decreasing residence
time.

Since the amount of CO^ produced can be measured just as
easily as the DOC decrease and the oxygen consumption, the
most expedient operating parameters can be obtained for
every individual case on the basis of the results with an
experimental filter, the establishment of the optimal ozone

-------
                         -  713  -
dose being often the main difficulty.  In an evaluation of
the experimental results from biological activated carbon
filters it is therefore advisable to observe and check the
relationships mentioned above.

If, after an ozone oxidation, the measurements and operation
of biologically adsorptive activated carbon filters are
carried out in this manner, the combined•process can prove'
to be a valuable aid in producing unimpeachable, naturally
pure drinking water.

-------
                       -  714  -
(1)  MAIER,  D.
    Wirkung von O2on auf die gelttsten organischen
    Substanzen im Eodenseewasser
    Vom Wasser _43_ (1974), 127

(2)  KURZ,  R.
    Untersuchungen zur Wirkung von Ozon auf
    Flockungsvorgange
    Dissertation, Universitat'Karlsruhe (1977)

(3)  SONTIIEIMER, H.,  W5LFEL, P.
    Amelioration de  la degradation biologique des eaux
    residuaires par  un traitement a 1'ozone
    Tagungsbericht Internat. Ozon-Kongress, Paris
    4-6  Mai  (1977)

(4)  EBERHARD,  M. , MADSEN, S.'r SON-THEIMER,  H.
    Untersuchungen zur Verv/endung biologisch arbeitender
    Aktivkohlefilter bei der Trinkwasseraufbereitung
    Engler-Bunte-Institut der Universitat  Karlsruhe,
    Veroff.  des Bereichs und.des Lehrstuhls fur
    Wasserchemie (1974), 7

(5)  EBERHARD,  M.
    Erfahrungen bei  der Anv/endung biologisch wirksamer
    Aktivkohlefilter          • •
    Engler-Bunte-Institut der Universitat  Karlsruhe,
    Veroff.  des Bereichs und des Lehrstuhls fur
    Wasserchemie (1975), 9, 283; English translation:
    EPA 60O/9-76-030 (1976)

(6)  SONTHEIMER, H.,  HEILKER, E., JEKEL, M.R., NOLTE, H.,
    VOLLMER, F.-H.
    The Mulheim Process
    J.  AIWA (1978) ,  393

(7)  GILBERT, E.
    Reaction of ozone with substituted aromatics and
    with their oxidation product^
    Proc.  of workshop on ozone'and chlorine dioxide,
    Cincinnati, Ohio, Nov. (1976);

-------
                         -715-
EXPERIENCE WITH BIOLOGICAL ACTIVATED CARBON FILTERS

M. Jekel
Introduction

The results presented here and the experience with the use
of biological activated carbon filters originate from a
common BMFT research project of the Rheinisch^-Westfalische
Wasserwerksgesellschaft (RWW) in Miilheim/Ruhr and of the
Engler-Bunte-Institut in Karlsruhe. The object of the in-
vestigations was to replace the classical process used for
the treatment of water of the river Ruhr - breakpoint .
chlorination, flocculation, and sedimentation, gravel and
activated carbon  filtration and sand bank filtration -  ,
by a direct process in technical plants, so that the high
degree of chlorination with its disadvantageous effects
on the water quality could be avoided. On the basis of the
experience in waterworks at the lower Rhine (1,2) and of
investigations at the municipal works in Bremen  (3) the
solution to the problem was seen in a combination o.f chemical
oxidation with ozone and biological-adsorptive treatment in
activated carbon filters (4).
Results from the pilot plants

Practical experience with this treatment was obtained both at
an experimental plant at Dohne waterworks of the RWW, which
had been running for two years, and at the main Dohne plant
itself, which had been converted to the new process 15 months
ago (5) .

-------
                         - 716 -
 In the pilot plant Ruhr water was treated by  flocculation,
 sedimentation, ozonization, and rapid filtration,  and  was
 passed through several activated carbon filters, various
 types of carbon being tested. In a smaller pilot plant,
 working in parallel, highly chlorinated flocculate discharge
 from the main plant was similarly treated with ozonization,
 rapid filtration,  and activated carbon filtration. The results
 of this parallel trial are shown in Fig. 1.
                             without breakooint chlorination
                              with breakpoint chlorination
                          8
                       10
12
                                        14
16
18
20
                     of water/1 activated carbon
Fig. 1
Influence of breakpoint chlorination on the
effectiveness of water treatment with  flocculation
and sedimentation, ozonization, filtration and
activated carbon treatment  (2.5 m at 1O m/h)
 This figure gives the percentage reduction of the UV ex-
 tinction at 254 run, as a measure of the removal of organic
 substances in the whole treatment process, against the
 throughput in m  per litre of activated carbon. The early
 breakthrough of the GAG filter with preceding breakpoint
 chlorination is a consequence of the high loading of the
 activated carbon with organic chlorine compounds, which
 hinders the development of an effective biological activity
 in the filter. Without breakpoint chlorination, however, an

-------
                          - 717 -
essentially better water quality was obtained, caused by
the effective microbial processes in the activated carbon
filter. These processes also ensured that, over a period
of about 14 months, the effectiveness of the treatment re-
mained essentially constant at 75 %, i.e. the upper curve
in the illustration can be extended to 4O m /I activated
carbon (5).

The biological oxidations in the GAC filter can be balanced
over the chemical parameters DOC, inorganic carbon, ammonia,
and dissolved oxygen. The following table shows as,an example
the variation of these four parameters in the GAC filters of
the pilot plant for the winter months January to March 1977
with low water temperatures and a relatively high ammonia
loading.
TABLE 1  Biological activity in activated carbon filters
         at low temperatures
Activated
carbon
LSS 2,5 m
ROW 2,5m
NK 12 2,5m
F 400 2,5m
BKA 2,5m
LSS 5,0m
ROW 5,0m
-A DOC
ppm
1,1
1,2
1,0
1,3
1,2
1,6
1,7
+ AanC
ppm
1,0
1,1
1,2
1,2
0,9
1,2
1,3
-ANH4
ppm
1/3
1,41
1/5
1/3
1/4
1/6
1/7
-AQ2
ppm
7,2
7,2
7,1
7,1
7J
7,7
7,7
Moan valuos: Jaa-Mdrz 1977
Mean water temn. : 6,8°C
Inflow (gravel 2'6 m9/1 DOC
filtrate): 1,53mg/lNH4
i 	 - 	 .. 	 — — , — . 	 . — .

-------
                           718 -
In the first 2.5 m of the filters, which operate at 1O m/h,
the amount of DOC removed is in general only a little greater
than the production of inorganic C, i.e. during this period
most of the organic substances removed were biologically
mineralized. At the same time an almost complete nitrifi-
cation of the ammonia took place, which was also responsible
for the high oxygen demand. In the second half of the 5 m
filters, however, the organic material was still predominantly
removed by adsorption, as was detected by the differences in
A DOC and A  inorg. C.

It is worth noting the really small differences in the bio-
logical activity of the activated carbons tested, which,
however, showed considerable differences in their adsorption
capacity. Moreover, further observation of the activated
carbon filters at the pilot plant showed that the nitrifi-
cation had virtually no effect on the biological oxidation
of the organic compounds. Furthermore, no significant in-
fluence of temperature on the biological activity could be
observed in the pilot p-lant.

The breakthrough behaviour of biological activated carbon
filters is influenced, among other things, by the adsorbed
biologically resistant substances. Both the removal perfor-
                                            3
mances of the pilot plant filters in g DOC/m  activated
carbon per day and the total loadings of the activated
carbons for the first 18 months of operation are given in
the following figure.

In. addition,  the removal performance was divided into a bio-
logical oxidation component and an adsorption componentr
calculated from the carbon loading. As shown in Fig. 3, an
average of about 75 % of the organic substances removed is
biologically oxidized, while the remainder, predominantly
resistant compounds, is adsorbed. Owing to the enrichment

-------
                         -  719  - " '
*
u.
%
•a
$ 175
*>
•H 150
•U >i
o *
10 -o 125
G) fl -
JS g
-u "--100
u
M-l O
0 ° 75
O &
O
i 5 so
.2 i 2s
**-* *Q
M N
*s


-
-
-
_









^-..-um--






—











___





""""""



i—

















1
-Adsorption
-biol. Oxidation








§
CJ *» <
W *~ "• ^
W z °
p-^-^^

i
1
^


, ,






















      1st half-year ! 2nd half-year 3rd half-year
Fig. 2  Performance and loading of biological activated
        carbon filters
 of these compounds, recognizable by the increasing loading
 of the activated carbon, the performance of the filters
 clearly falls off, particularly in the third half-year
 period. Simultaneously, a clear improvement in the quality
 of the raw water occurred, so that the DOC in the influx
 was decreased from about 2.5 to 1.8 ppm. This certainly
 also intensifies the decrease in performance. The measure-
 ment results indicate that the degradable substances are
 at first adsorbed and only then biologically mineralized.
 The relatively high proportion of about 75 % of biologically
 oxidized substances furthermore permits the conclusion that
 the operating time of biological activated carbon filters
 in the treatment of Ruhr water is prolonged by a factor
 of approximately 4 in comparison with pure adsorption.

-------
                          - 720
 Experience with the treatment at  the Dohne waterworks

 On the basis  of the results with  the pilot plant, the  Dohne
^waterworks were converted to the  new treatment process in
 the spring of 1977, as shown in the following flow diagram
 (4):'
               Mixing Tank   PUHMOK       Gasification Double- Activated
                                       Tank    layer  Carbon
                            Ozonized Air         Filter  Filter
             Pumps
1
j

•%

„• 	 /
s


\,^.,_, ...



L

/

^

v
                                            A?-
                                            Pumps
                                        Ozonized
                               Generator  Air
      Safety chlorination
Collecting Percolation Withdrawal
  Well     Well      Well
                                               Filter
Fig. 3  Flow diagram of the Dohne works
 The Ruhr water is directed into  a small mixing chamber .for
 preliminary  oxidation with about 1  ppm of ozone. Poly-
 aluminium chloride is added at the same time for flocculation.
 Die preliminary ozonization with an immersion gas tube :results
 in clear water after the solids  have been removed in  a pul-
 sator.  On average about 2 ppm of ozone are introduced into
 the gasification chamber. If necessary, additional pure  oxygen
 can be mixed in before the filters to cover the consumption in
 the subsequent rapid and activated carbon filters. The bed

-------
                         - 721  -


 length of the activated carbon filters was increased from
 2 to 4 m to maintain a sufficient residence time at a velocity
 of 22 m/h. These were set into operation in November of
 last year with virgin activated carbon.

 The following table shows how the conversion to the new
 treatment affected the quality of the drinking water.
TABLE 2  Effectiveness of classical and new_treatment
         at Dohne waterworks, RWW, Mlilheim/Ruhr
New treatment, without
' UV
m
Ruhr
after
after
after
after

f locculation
ozonization
filtration
soil passage
8
4
3
3
2
activated carbon filter (July-Oct . 1 977 )
Ext. DOC NH£ Colony E.coli/
1 mg/1 mg/1 count/ml 100 ml
.6
.9
.2
. 1
.3
4
3
3
2
1
.O O.79 963O
.3 0"._43. 3510
.1 0.43
.7 O.O3 144O
.2 O.O1 19
1550
16
-
1
<1
Classical treatment - - .
(1975)
Ruhr
after
after
after
after

f locculation
ozonization
activ. carbon
soil passage
6
4
4
4
3
.8
.5
.4
.O
.1
4
3
3
3
1
.O - '• • •
.2
.2
.O
.8





 The mean values of some parameters in the new treatment
 without activated carbon filters are compared here with
 the values of the earlier classical process in 1975,  when

-------
                        - 722 -
 the quality of the untreated water was similar.'The oxi-
 dation effect of the ozone is noticeable, by means of which
 resistant material is converted to biologically  degradable
 substances. The improved purification effect of  the soil
 passage is also due to this factor. The fact that the rapid
 gravel filters are also biologically active is obvious from
 the extensive reduction of ammonia and the re-population
 of the filtrate with bacteria. The numbers of colonies
 decrease in the soil passage to two-digit numbers, since
 the degradable substances have been practically  completely
 removed.
 With the inclusion of the enlarged activated carbon filters
 a large proportion of the purification effect of the soil
 passage was shifted to the plant.  The behaviour of the newly
 installed large filters is shown in the following figure, in
 which the mean values of the removed DOC and the inorganic
 carbon formed in the whole activated carbon filtrate are
 plotted against the time of operation.
c^
E
M
0
C „. _
•H 0.6
^3
X °
Q
^ 0.2



_
~


-








Dec.
1977







Jan.







'Feb.
T Ainorg. C
1
I
I
, , A IW*



1 I 1 H 	
March Aoril ' May ' June ' July'
1978
Fig. 4  Behaviour of the newly installed biological
        activated carbon filter at Donne waterworks

-------
                          '-• 72-3  -                  ^


  In  the first three months with .the water temperature below
  8   C  the activated carbon filters showed practically no
  biological activity. The A DOC values indicated a really
  early breakthrough of the organic substances, since the
  carbon was already becoming increasingly loaded. With the
  higher concentrations of adsorbed degradable substances
  and with rapidly rising temperatures in the spring the
  biological oxidation set in, which from May clearly covered
  the substance already adsorbed. The activated carbon was
  regenerated biologically and from this month on displayed
  an  improved purification effect.,

  Exhaustive investigations on the"bacteriological nature of
  the activated carbon 'filtrate were carried out in the large
  plant,  because this aspect plays an important role in a
  direct input into the .-distribution network. Since -four, types
  of  activated.carbons-with-different adsorption capacities
  were  used in the large plant, the filtrates showed sometimes
  considerable differences inVthe.DOC values. The effect of
  this  on'the colony'counts determined in-parallel  (incubated
  for 48 h at 22  C on nutrient gelatine)  is shown in the
  following figure.   ••••."".•.•.
  o
  (N
  (N
  ~~»
  JC
  CO
  •3-
  o
  o
140
120

100

 80

 60

 40

 20
                                        * X
 O Dec,77
 a Jan.78
 a Feb.78
 • March 78
- A April 78
 • May/June 78 a
 x July 78
 	I
              0.5
                   1O
                         1.5
                      DOC in .mgll
2£>
2.5
                                                        3.0
Fig. 5
    Influence of the  DOC  in.the filtrate of biological
    activated carbon .filters .on their bacterial
    population   '        ...•••.

-------
                        - 724 -
The geometric mean monthly coloriy counts are here correlated
with the  corresponding mean DOC values of the individual
carbon filtrates.  While in the first month of operation,
December 1977, with still' scarcely any developed biological
activity, no relation between bacterial population and the DOC
can yet be distinguished, in the following months with
increasing biological activity of the active carbon filters
a dependence of the bacterial population on the organic
loading of the filtrate is established.  Remarkably, greater
deviations appear in May and June, when a different compos-
ition of the organic substance during the growth of algae was
clearly present.  From these results it may be concluded that
a larger proportion of biologically resistant compounds
occurred at this time.               •   "
 From these results it follows that a good adsorbing activated
 carbon,  which also removes degradable substances more effi-
 ciently, produces lower colony counts in the filtrate. The
 mean values themselves show predominantly two-digit and three-
 digit colony counts. However, if the ozone dosage is increased,
 the experience with the Dohne:waterworks indicates that the
 bacterial counts become considerably higher.

 A natural far-reaching elimination-of bacteria  from the
 activated carbon filtrate can still be achieved, according
 to trials with a rapidly operating slow sand filter, at a
 velocity of 2 m/h, the colony counts being then reduced on
 average  to single-digit values. (6),

 With the development of an effective microbiological activity
 in the spring and summer of this year, a mass development of
 nematodes was observed in the rapid and active carbon filters.
 The reasons for this are to be found mainly in the long running
 times of the gravel filters,  which,  because of the very good

-------
                       - 725 -
quality of the clear water after floeculation, were one week.
On the other hand, for technical reasons the gravel filter
in the Donne plant could be backwashed only with J±ie rela-
tively low speed of 2O m/h.  ,

According to running experience, this problem can be solved
by frequent washing of the> filter, so that the running time
should not be more than four days, the reproduction cycle of
nematodes. This applies above all to the time of increased
water temperatures in the summer, when a continuous obser-
vation of the backwash -water is recommended to prevent a
mass development by frequent and vigorous backwashes.
However, the back-flushing speed should not be so high
that the microbiological activity of the filters is impaired,.
Summary              • •  • •   -    -

The experience with biologically working activated.carbon
filters in the treatment 'of Ruhr water can be essentially
summarized in the following "points:

1)  The high biological activity of the activated carbon
    filters prolongs the running•time about fourfold in
    comparison with pure adsorption.

2)  The bacteriological nature of  the activated carbon
    filtrate will be satisfactory  when a far-reaching-   -
    removal of biologically degradable substances is
    achieved.        .,-•,*-          •           ,

3)  Reliable operation of biological filters can be
    achieved by means of a suitable backwashing technique

-------
                        -  726 -
(1)  HOPF ,  W .                  -...••
    Versuche  mit Aktivkohlen zur Aufbereitung des Diisseldorfer
    Trinkwassers
    gwf-Wasser/Abwasser 1O1  (196O), 14, 33O-336

(2)  HOPF,  W.
    Zur Aufbereitung rnit Ozon und AktiVkohle
    gwf-Wasser/Abwasser V1_1  (1970), 13, 156-164

(3)  EBERHA'RDT, M. ,  MADSEN,  S., SONTHEIMER, H.
    Untersuchungen  zur Verwendung biologisch arbeitender
    AktivkohlefiIter bei der Trinkwasseraufbereitung
    Publication Wasserchemie, Engler-Bunte-Institut,
    Univ.  Karlsruhe 1_ (1976); gwf-Wasser/Abwasser 116
    (1975),  6, 245-247

(4)  SONTHEIMER, H., HEILKER, E., JEKEL, M.R., NOLTE, H.,
    VOLLMER,  F.H.
    The Mulheim Process
    J.  AVJWA 10_ (1978) , 393-396   	

(5)  HEILKER,  E., JEKEL, M., SONTHEIMER/' H.
    Biologisch-adsorptive Trinkwasseraufbereitung in
    Aktivkohlefiltern
    DVGW-Schriftenreihe Wasser, Nr. 1O1,  zum BMFT-DVGW-
    Statusseminar "Heue Technologien,  Hannover, Jan. 1978
    To  be  published

(6)  SCHALEKAMP, M.
    Die V7irksamkeit yon schnell betriebenen Landsamsand-
    f iltern                   .           .•
    Publ.  Wasserchemie, Enaleir-Bunte-Institut, Univ. Karlsruhe,
    5  (1971),  3, 49-66

-------
                           -  727  -_
THE USE OF COMBINED CHEMICAL AND BIOLOGICAL  OXIDATION
PROCESSES       .                          .
P. Schulhof
Before I talk about- the processes combining 'Chemical" and  bio-
logical oxidation I should like to dwell, briefly  on  what  I
would say .about the -.papers given in- the, course of this  "  , ,
symposium if I were one of the men responsible for the  con-
struction or modernization of the drinking-water-treatment <
plants in France.

I would first have remarked on the incredible advances  in '•
analytical methods in recent years.  I-would, have been-  parti"
cularly surprised by the absolutely thorough knowledge
attained within a very, short .time with  the aid, of these.  t  •_
methods on the subject of the oxidation of polluted  water;

However, my enthusiasm would have -been  somewhat dampened ;:by
the fact that the majority of cases dealt with studies  ,  . •
on isolated products or'-substances, while many of the speakers
have made reference, to the competition  situations that  can.  ,
arise in the course of oxidation'reactions in mixtures. Let us
not forget that raw river water-is-a particularly complex
mixture 1

However, the agreement of the presented results and  in  parti-
cular of the questions that have been put would have disturbed
me too.  My concern would have "'been intensified by the  fact
that the sanitary and hygienic significance of all the by-
products mentioned is still far from known.  After several
years we are still debating about the simplest of them,
chloroform and, according' to your remarks this represents
only the tip of the iceberg.

-------
                           - 728 •-


It must be added that,the  industry evolves new compounds from
year to year, indeed  at least as many as those that have been
mentioned during the  last  three days.

On the other hand, my concern would have been somewhat
abated by the consideration that the studies are often per-
formed under extreme conditions: very severely polluted
water such as Rhine water  or oxidation with very massive
doses as in the United States.  The average reality in
moderate France is perhaps less gloomy.

Finally, I would now perhaps understand better the wisdom of
the German standards cited by you, recommending the use of
waters low in man-made substances. • Within the wider framework
of the EEC I would also understand the reasons for the caution
of the guidelines established 'in Brussels for the quality of
the raw water about to undergo treatment.

To return to everyday matters in France, however, the con-
struction of treatment plants must be developed further and
the plants must be run with the water available.  The plants
for treating the severely  polluted waters in the Paris area
must be modernized.  What must be done here?

From these days of study some rules have emerged clearly that
normally should be a matter of plain common sense.

For example, the fact that in the case of a polluted water
the first treatment process to be put into action should not .
be an aggressive oxidation.  Therefore I would strive towards
a gentler oxidation, i.e.  biological, and one that is as
natural as possible.

I concede that I do not have available a site suitable for a
soil passage.  These do exist in France, but by no means
everywhere.

-------
                          - 729 -
First  of  all  I would  investigate  whether  a storage reservoir
can be installed  for  the  raw water.   This biological reactor
hardly mentioned  this morning ••-  is  at once a clarifying tank
and a  safety  stage, thus  offering a  whole series of simul-
taneous advantages.
If I had no room for  a reservoir  of  this  kind  I  would look
for a different biological reactor,  but which  one?   This
would pose considerable problems.

Let us continue.   A physical and  chemical clarification line
is put in.  For the pre-oxidat.ion. I  would use  ozone  in a
moderate dose.  After allr we have seen that preliminary
ozonization is a logical supplement  to flocculation.

Then there is the viricidal ozonization,  still indispensable
for the treatment of polluted water.  Tts logical  supplemen-
tation was described this morning.  This involves a second bio-
logical reactor that,followed by  a weak final  chlorination,
should protect the network.

Perhaps I would have had certain  difficulties  in designing
the ozonization with a minimal viricidal dose.   I  have main^
tained that the addition should be continuous, neither too
strong nor too weak and extended  over a really long  period.

All this is very rough, and in my special case I would defi-
nitely construct a pilot,plant, which would present  no undue
difficulties.

On the other hand, I would find it much more difficult to
determine the biological reactor  to  be installed.  For what
is to be done when the problem is not one of modernizing an
old plant that already makes use  of  slow  sand  filters or
activated carbon filters?

-------
                           - 73O -
It seems highly unlikely that when a new plant is to be built
the slow sand filter technology designed almost a century ago
in a completely different context or the activated carbon
filter technology developed 30 years ago for the purposes of
 f
dechlorination and the prevention of undesirable taste are
ideal'for a biological reactor.

This is the real problem that I wished to come to with these
lengthy preliminary remarks.  (fhe technology of chemical
oxidation has apparently reached the age of maturity.  The
most recent of these techniques, ozonization, has grown up
in Prance over the last 15 years. In my view the same cannot
be said of the technology of the biological reactors, which
is still in its infancy.

In the years to come we shall need at least two different
types of such biological reactors, one for raw water and one
for clarified water.

I believe this is the challenge that progress in analysis
places before the technologists.  Only when these developments
succeed will the studies presented here make possible spec-
tacular advances in the treatment of water.

The technology of the biological"reactors could, and this is
a thoroughly classical conclusion towards the end of a sym-
posium, become the very likely arid universally useful theme
of another symposium.         "      '

-------
                          - 731 -
A. -Bousoulengas  (Greece)
1. Most of Greece's small cities and villages use ground
water for drinking purposes. In bigger cities surface
water, or both ground and surface water is used.

2. Since 1958 chlorination has been established by Sani-
tary Regulation as the disinfection means for drinking ,  •
water supplies. There should always be 0,2 mg/1 free C12
present at any point in the distribution network.
This is checked regularly by taking samples  from a nujn- -
ber of points, depending on the size of the  network .in
question.

3. Sanitary Regulation of 1968 "On the Quality of Drin-
king .Water" determines the physical, chemical and micro-
biological characteristics that drinking water should,  • •
have. It determines ma'ximum allowable concentrations -for,
various inorganic substances  (Ag, As, Cd, Pb, ,F  etc.) . •
and organic substances as well.            ~    .   .   ;•-•••
Regarding organic substances and their products  (NH,,. NO!,
NO2 etc.) there is a recommendation for their guanti-:  "
tative determination.  ' ' '                          ,-•••.-

4. There are no regulations determining the  treatment
process. However, the above-mentioned sanitary regula-
tion prohibits the use of'a water source if - it ,is found •
to contain a series of 'substances in concentrations       •:
higher than those determined by the regulation. These
substances include sulphates, carbonates and other inor-
ganic substances and organic ones such as detergents ,  ,
and phenolic compounds.                         .     ..

-------
                          - 73-2'.V  •

5. Research is carried out by various  state  institutes'"''1
and university laboratories on mainly  applied problems
concerning drinking water.

6. If a water source is found suitable for .drinking
use, restrictions are applied by- regulation  or decree
prohibiting the discharge of any effluents or the  dis-
posal of any materials into the source or into the wa-
terways feeding this source - lake etc.

-------
                          - 73.3'.-
L. Coin' • (France) -
QUALITY OF RAW WATER:
France is trying to use raw water of adequate quality.
However, as a result of modern developments and changes
in the habits of the consumer in our industrial society -
despite the fierce battle being fought against environmental
pollution - certain correcting measures seem inevitable.
They would be:

-  either on a. quality level, generally by choosing
   different locations as raw water sources, or by making
   new water reserves accessible;

-  or on a treatment level, by reconstructing existing
   plants and by introducing the most recent adsorption
   agents, or by modified oxidation processes.

We are therefore awaiting with interest the realization of.
joint regulations on the quality grade of raw water permitted
to be used for drinking-water treatment.
REGULATIONS ON THE CONTROL OF DISINFECTION

There are two forms of control:
1.  performed on-site by the waterworks;
2.  spot-checks carried out by the Department of Public Health,
   . Dates and guidelines are laid down by the C.S.H.P.F.
    (Conseil Superieur.d1Hygiene Publique de France).

-------
                          - 734 -
                            s - f  *

On account of the special  guidelines on maximum concen-
trations of micro-pollutants and similar substances, drawn
up in Brussels but not in  force yet, France has modified
the regulations. These modifications have partly been
legalized already/ for instance concerning those regu-
lations which were only slightly changed, e.g. for heavy
metals. Organic micro-pollutants, not mentioned previously,
are now included in the programme of treatment plants in•
densely populated towns.
NECESSITY AND VALUE OF SAFETY CHLORINATION:
This is a controversial question. In smaller residential
areas drinking water is supplied to the consumer without
previous treatment, taking into account water protection
areas and issueing certain restrictions. In densely popu-
lated areas chlorine is added to the water. The real problem
is subsequent chlorination after ozonization. In Paris, for
instance, the excessive chlorine is neutralized again before
distribution. Our ideas on raicrobial control will certainly
have to be revised; Concerning fecal germs, the present
strict control measures should be retained. Improvements
seem necessary, however, in connection with the significance
of the fluctuation of the saprophyt numbers. A chlorine con-
tent of O.O5 ppm to O.I ppm at the most, as it is laid down
in Prance, covers short-period risks,; it should, however, be
increased in times of epidemics.
BREAKPOINT CHLORINATION - yes(or no?
After having been in use for many years, breakpoint chlori-
nation is about to be abolished in France - and with good
reason.

-------
                           - 735 -
REGULATIONS''ON"METHODS: OF TREATMENT:'^
This is a complicated question. The Offices of Public Health
decide on the type of treatment used for producing drinking
water from raw water. However, this decree does not come into
effect in those cases where the size of the project requires
the decision of the C.S.H.P.F. This point in particular will
be treated in the ruling still under consideration, in complete
agreement with all parties concerned.
RULES FOR APPLICATION AND FUTURE RESEARCH:
All the various regulations have been taken very seriously
in France, especially as concerns the treatment of raw water
Questions concerning the distribution network are governed
at present by hygiene regulations of the Departements. This
presents a serious shortcoming, which will surely be over-
come in the future.

As far as research is concerned, the various methods of
treatment, their development and improvement remain very
important. Epidemology will forcibly include the effects of
the distribution system on the water  quality at the con-
sumer ' s tap, and here the responsibility also lies with the
consumer himself, because he uses the water supplied to him
and thereby changes the quality himself. This is a decisive
point,  and the quality of the material used for domestic
plumbing is also gaining importance in this connection.

-------
                          - 736 -
T.A. Dick (United Kingdom)
1.  Sources and Treatment of Water

In the United Kingdom, one third of the potable water is
derived from underground supplies, one third from upland
surface supplies  (lakes, reservoirs and streams) and one
third from lowland rivers. The proportions vary conside-
rably in different parts of the country and in the drier
eastern areas there is little choice but to draw water
from rivers which may contain a substantial proportion
of waste water, that is, water which has passed through
a sewage treatment plant. Sewage treatment plant effluents
are not chlorinated before being discharged to UK rivers.

Underground water supplies and upland surface water sup-
plies are generally of high quality and require only the
conventional treatments. For surface waters from lowland
rivers it is recommended practice in the UK to store the
water in reservoirs, as a safeguard against fluctuating
river flows, possible accidental contamination and to ob-
tain self-purification benefit. The water then receives
treatment by slow sand filtration, or coagulation and ra-
pid gravity filtration or both. The water then passes to
a closed service reservoir before distribution or may go
directly into supply. Pre-chlorination treatment of the
raw water before or during treatment is fairly common.

With few exceptions, all public water supplies in the
United Kingdom are chlorinated after treatment by conven-
tional methods. As a general policy, the degree of final
chlorination is such that O.1 to O.2 mg/1 of free chlorine
remains at the end of 3O minutes contact.

-------
                           - -737  -
2. Quality of Water           .  •', , <.  '. •••. LtVi ;; •><•',  ,A '•'

There are, at present, no mandatory standards  in the UK for-
the quality of water put into supply  other than that the
water shal-1 be  'wholesome',  which is  generally accepted
as meaning "clear, palatable and  safe".  In practice, the
quality of 'water put into supply  follows, the recommenda-
tions of  the. 1970 World Health  Organisation European Stan-
dards, and for  bacteriological  quality,  the recommendations
given in  a UK Government document Report 7.1, "The Bacterio-
logical Examination of Water Supplies".  -There are n'o- man-
datory regulations for plate counts for drinking waters in
the UK. However, plate, counts are carried .out by many of
the water suppliers to assess changes in the general qua-
lity of water in.the treatment  plant  and supply system,
particularly wheri the raw water has been derived from sur-
face sources. The 37  plate  count is  generally used as
pollution indicator but the  total plate .counts at 22.  were
of. particular value during  the  1976 drought year to" ensure
that there was  no in-leakage of. contaminated water into
-distribution mains.
3. Present UK Policy

The United Kingdom  is well  aware  of  the work .discussed at
the present Conference  but  its  approach to the establish-
ment of water regulations and policy is at present direc-
ted more  to establish whether a health risk exists,  rather
than making any  fundamental change  in treatment policy in-
to unknown areas  of risk. The UK  is  well situated for such
research. It has  a  wide variety of  situations  where there
is reuse  of water and where the Water Research Centre -and
the 1O Regional Water Authorities have accumulated exten-
sive data on water  quality.  We  have  excellent  .health data

-------
                          - 73'8 - "

aecumulated'-'over'-many ye*ars and • we have an intensive .pro-  .
gramme 'on micropollutant identification and mutagenic
screening of waters and concentrated water samples. De-
tailed statistical examination of the data is actively in
progress. There is also a national programme for the de-
termination of trihalomethanes in drinking water.

We believe this type of work is of fundamental importance
In establishing whether our policy on reuse of water and
on chlorination is sound. It is encouraging to note, for
example, that the national data for the incidence of death
from cancer of the stomach and the bladder have fallen
significantly between 1970 and 1975 for adults between 45
and 64 years. I should also- add that the UK has another
drinking water problem concerning reduction of lead, pri-
marily in the older houses with lead pipes in soft water
areas, and at present, we regard this as our number one
priority for action.
4. Catchment Control

As a final point, the UK believes that there is consi-
derable advantage in reducing the input to raw water sour-
ces of organic materials rather than trying to remove
them from the water during the treatment process. Some of
the UK Water Authorities are now intensively monitoring
the use of organic materials in factories, to see what
is used in the process and what can be lost from the pro-
cess during manufacture. At the same time, the effluents
leaving the plant and the sewage works are analysed and
also the river itself to establish a 'balance1 on the
'missing' organic material. The amounts can be small but
very significant. Each type of industry is being examined,
and attention will also be given to commercial bodies

-------
                       •'  --739 -  ,

(e, gV "laun'drie's, dry-cleaners  etc)'  and to the--domestic
use of chemicals. The system,  known as catchment control,
requires a great deal of patient work but can be very  '._
successful in reducing, un.de s.ir able  micropollutants from
factory processes, particularly_where less harmful ma-
terials can be used in- the process.

-------
                         - 740 -
1.." Heinohen .(Finland^
The water supply of the largest towns in Finland is de-
pendent on surface water sources.- In treatment the water
passes through coagulation with aluminium sulphate at a
suitable pH («6), clarification and rapid sand filtra-
tion. Chlorine is the chemical,-used for disinfection and
generally also for the .oxidation,of substances affecting
taste and odor.

The ammonia content in: river waters, used as raw water
supplies and carrying a pollution load of domestic waste-
waters, rises in the winter to such values that break-
point chlorination of, NH, is necessary. Also spring run-
off from fields during the flo.od period causes taste and
odor disturbances that are not: removed by treatment pro-
cess. They are reduced ,by .superchlorination and the use
of powdered activated carbon. Blooms of algae'and micro-
organisms in lake basins especially in the summer often
cause taste and odor disturbances in normally treated
drinking water. Chlorine dioxide has been used to elimi-
nate these and experiments have even been made with KMnO,
instead of, or in addition to chlorine oxidation. The
most effective way of combating algae in watercourses and
at water intakes has proved .to be treatment with copper
sulphate.              .          ,

The Finnish National Medical Board has given standards
for drinking water; these were last revised in 1971. They
are principally based on the international drinking water
standards of WHO dating from ,1963 with a few points taken

-------
                            741 -
from the WHO European area standard Of  19"70.^Gft special
interest with re-gard to the conditions  prevalent  in Fin-
land are the norms for KMnO.-consumption in order to  li-
mit organic matter in the water  (lower  limit 20 mg/1  and
upper limit 40 mg/1), forj-NH., as. an indicator of  pollution
(O.2 and O.5 mg/1), as well'as for Al-residue, characte-
rizing the treatment process  CO.5 - 1.O mg/1). Of organic
compounds limits have only been  set for phenols and an-
ionactive detergents,- bait--not for example for biocides
or any of the general parameters  (e.g..  CCE, TOO...-

The bacteriological standards give limit values to fecal
streptococci in addition to .conforms and thermotolerant
coli bacteria*         -"    ,-•;-.•           .   .

The most common difficulties  in  surface water .treatment
are due to the water's fairly high humus content, some-
times also to similar e'ffects -caused  by wastewaters from
pulp production in the-.wood-processing  industry.  The  re-
moval of organic matter is  incomplete in Al-coagulation
(TOC residue >4 mg/1) and a fair amount of aluminium  re-
mains in the water  (>0.3 mg/1);  the high chlorine  demand
and its corresponding use result in the formation of  con-
siderable amounts of organic-chlorine compounds obser-
vable both as taste and.odor  disturbances as well as  the,
incidence of CHC13  (>5O ug/1),:observed in analyses.
Taste and odor problems are intensified'during  the - winter
time with break-point chlorination to destroy the ammonia.
Similar problems are experienced sporadically also during
periods of spring flood and warm summers.

The measures used -at the major waterworks in Finland  to
solve problems arising in water- treatment are .principally
the following:

-------
                         - 742 -
1, Improving the quality of  the raw water  sources  in use

Water conservation, dilution with water  transferred from
neighboring watercourses, and  impoundment  before taking
the water into treatment plants- have been  the methods
most commonly used. All of the -above-mentioned have been
used especially by 'Helsinki City -Waterworks.

2. Transferring water intake to sources  of a better quality

The numerous lakes in Finland  form" alternative water supplies
of good quality and abundant"quantity to the polluted river
or lake waters presently in use.- The Helsinki metropolitan
area on the southern coast as well as several communities
north of it are presently engaged in -a- project of  construc-
ting a rock tunnel 12O km long from the'  southern end of
Lake Paijanne, a large lake basin -in Central Finland. The
tunnel will be completed in  1982. Several  other smaller wa-
ter transfer projects have been completed  or are in the
planning stage for the water supply of other major Finnish
cities.

3. Improving present treatment methods

The methods mentioned above for the improvement of raw
water quality already aim at diminishing the substances
causing taste and odor as well as other  disturbances in
drinking water. The treatment process proper has been im-
proved both by increasing the effectiveness of chemical
treatment (increased use of activated carbon and chlorine
dioxide) and by making changes in the order of the various
phases of the process so that the formation of disturbing
substances has been diminished (e.g. decrease in the for-
mation of chlorinated hydrocarbons by transferring the
main chlorination point to after coagulation and filtra-
tion) .

-------
                          - 743/r

4.  Developing new .treatment methods

The trend is to substitute chlorine oxidation with ozo-
nization. Helsinki and its neighboring municipalities
will begin ozone treatment already in 1979.' Several other
towns are at-present carrying•out study programs on its'
use or are contemplating the starting of such programs  in
the near future.

Activated carbon filtration after chlorine oxidation has
been studied with large-scale pilot plant test runs in
Helsinki and its introduction is at the moment studied  at •
Turku, one of the largest cities. next to Helsinki, to relieve
local water treatment problems.

The test runs in Helsinki showed as drawbacks for the me-
thod the rapid decrease in - adsorption capacity  (during  a
run of 3-5 months) showed-by measurements, and a corres-
ponding rapid deterioration as well as large-fluctuations
in taste and odor characteristics. This led to the decision
to abandon this method of quality improvement and to adopt '
ozonization, which had consistently given good results  in
the test runs, as an additional method to make the treat-
ment process more effective.'   '           '               '•

-------
                         - 744 -
Victor J. Kimm  (U.S.A.')
Legislative control of contagious or infectious disease
first began in the United States with the National Quarantine
Act of 1878. Regulations developed under this Act were inten-
ded primarily to maintain sanitary; drinking water aboard vessels
However, in 1912, due to severe outbreaks of intestional disease
among passengers aboard steamships on the Great Lakes, the
Treasury Department issued rules which required State certi-
fication that drinking water aboard interstate vehicles would
not cause disease. This concept beicame the basis for the 1914
Treasury Standards and the subsequent modifications knows as
the U.S. Public Health Service Drinking Water Standards.

Early in 1968, a series of bills"were introduced in Congress
for the general protection of drinking water. Eventually the
Safe Drinking Water Act of 1974 was passed, and it applies to
all public water systems serving 25 persons or more on a
regular basis, for a total of about 2OO,OOO,OOQ Americans.
This Act mandates the U.S. Envirorimental Protection Agency
to develop primary drinking water !regulations containing maxi-
mum contaminant levels for microbiological, chemical and radio-
logical constituents that affect the public health.
NATIONAL INTERIM PRIMARY DRINKING WATER REGULATIONS
The Interim Regulations establish Maximum Contaminant Levels
(MCL) for coliform bacteria, turbidity,:ten inorganic chemicals,
six pesticides and radiological contaminants  (Table 1). As of
June 24, 1977, water utilities are required to conduct periodic
monitoring at a prescribed frequency to ensure compliance with

-------
                         -  745 - ,
TABLE T  The maximum contaminant levels for constituents
         in the National Interim Primary Regulations
 Constituent
         Level
 mg/1 unless specified
 Biological Parameters

 Coliform bacteria
 Turbidity
 Inorganic Chemicals

 Arsenic
 Barium
 Cadmium
 Chromium
 Lead
 Mercury
 Nitrate (as N)
 Selenium
 Silver
 Fluoride
 Organic Chemicals

 Endrin
 Lindane
 Methoxychlor
 Toxaphene
 2,4D
 2,4,5TP Silvex
 Radionuclides

 Radium 226 and 228 (combined)
 Gross alpha particle activi.ty
 Gross beta particle activity
 1  per 1OO mi (mean)
 1  NTU (Waiver to 5 NTU
        possible)
 0.05
 1
 O.O1O
,0.05
 0.05
 0.002
10
 O.O1
 0.05
 1 .4-2.4"
•0.0002
 0.004
 0.1
 0.005
 0.1
 O.O1
 5 pCi/1
15 pCi/1
 4 millirem/year
   Based on annual average air "temperature

-------
                         - 746 -'                                . ,


the regulations and '-to notify the public i,f , the ^standards, ,are , ( .
exceeded. The Safe Drinking Water Act also provides that States
will develop their own regulations and some States have more
stringent requirements than those in the Interim Primary
Regulations.

The Maximum Contaminant Levels for coliform bacteria and
turbidity are essentially those contained in the 1962 Public
Health Service Standards  (Table 2). It is generally agreed
that these MCLs are adequate to protect the public from
infectious disease transmitted by recent fecal contamination;
however, there is data to suggest that the coliform testing
procedures may not be entirely effective as an indicator of
certain diseases of viral and .protozoan etiologies. The
apparent problem with the indicator procedures is,not whether
those particular disease-producing organisms are found in
water contaminated with fecal material, but rather that a
number of viruses and protozoans can survive in water for
longer periods than the coliform indicators. Some viruses
and protozoans also seem more resistant to disinfection than
coliforms.

At the present time there are no national disinfection stan-
dards in the United States. Drinking water disinfection is
hot mandatory nor are there restrictions on the quantity and
type of disinfectant that can be used. The MCL of a monthly
mean of 1 coliform/1OO ml is the sole measure of microbio-
logical quality, but the means of achieving acceptable coliform
levels are not prescribed. Monitoring frequencies based upon
the size of the population served range from a minimum of
1/month for populations of less than 1OOO to SOO/month for
populations larger than 4,690,OOO. There is, however, a
provision in the Interim Primary regulations that allows

-------
                          - 747' -
TABLE 2  Provisions" In-the'National-' IntWrim  Primary
        • Drinking ..Water Regulations  affecting  the -. -
         presence of microorganisms
 -1 .  Coliform -bacteria -  1 coliform/10O ml of water
    .. a)  A supply may substitute chlorine residual
         determinations for not more  than 75  % of the
         required number of coliform  analyses if
         0.2 mg/1 free chlorine is maintained.
 2.  Turbidity - 1 nephelometric turbidity unit  (NTU).
     The State may allow up to 5 NTU if water does not
     cause a risk to health and the Increased turbidity
     does not:      .           .          -         .     •

     a)  interfere with disinfection; •
     b)  prevent maintenance of an effective disinfectant
         agent throughout the distribution system;
     c)  interfere with microbiological determinations.
States to permit the substitution of chlorine residual analyses
for up to 75 % of the monthly coliform sampling requirements.
If that option is selected, a free chlorine residual of at
least O.2 mg/1 must be maintained. Should the chlorine resi-
dual fall below O.2 mg/1, immediate sampling for coliform
bacteria is required.

The value of disinfectants in controlling pathogens in water
is unassailable, but, in one sense, disinfectants and their
by-products, and particularly chlorine and chloramlnes, are

-------
                          -  746  -

contaminants of increasing public health concern. They are
the most widely used synthetic chemicals in water treatment
in the US and they are used at relatively high concentrations.
EPA finds itself in a difficult position in prescribing con-
trols because there is very little .information currently
available on the human toxicology of chronic exposure to
disinfectant chemicals, their degradation products, or their
reaction by-products with other contaminant chemicals in
water. That is an appalling fact considering some of these
disinfectants have been in use  for over 60 years.

The issue concerning the misuse of disinfectants has become
particularly acute in the past three years with the identifi-
cation of chloroform and other trihalomethanes in chlorinated
drinking water. But trihalomethanes are not the entire problem
by far; it is only the relative ease of identification and
quantification that has caused interest to concentrate on them.
The trihalomethanes should be considered indicators of the
existence of a host of undefined and perhaps undefinable
oxidized and halogenated chemicals that are introduced as a
result of chlorination. The same questions can be raised about
any disinfectant under consideration, be it chlorine, chlora-
mine, ozone, chlorine dioxide, or iodine.

The Environmental Protection Agency has recently proposed
regulations to limit trihalomethane.concentration aimed at
minimizing risks from unnecessary exposure to the by-products
of the disinfection process. High doses of disinfectants
should not be used to provide chemical oxidation treatment
unless it is part of another process which would control the
chemical by-products produced. In high organic water, water
treatment processes should be applied that involve purifi-
cation to reduce precursor levels before application of a
disinfectant.

-------
                          - 749  -  -.-"••

These proposed 'regulations further conclude that it is ne'cessary
to take steps to limit and minimize' trihalomethanes in drinking
water by means that would not interfere with the maintenance of
pathogen control. The current proposed regulations include an
initial limit on total trihalomethanes (the sum of chloroform,
bromodichloromethane, chlorodibromomethane and bromoform) of
0.10 mg/1 for approximately 400 public water systems serving
populations greater than 75,OOO persons. Only monitoring is
required in smaller systems (10,000 to 75,000), and systems  '
with less than 1O,OOO are not covered. The initial standard
was selected based on current feasibility and is not to be
construed as a "safe" level. This standard will be reduced
and population coverage increased as experience is gained.

To assure that any steps taken to reduce THM concentrations
in drinking water will not increase the possibility of
microbial contamination, additional microbiological moni-
toring is proposed for•a water system that is modifiying
existing treatment practice. Standard Plate Count  (SPG)
determinations must be made at least daily both at the treat-
ment plant and in the distribution system, for one month
before and six months subsequent to the treatment change to
assure that no degradation of water quality occurs. Analyses
prior to any treatment change are intended to provide a base-
line to which subsequent effects can be compared. The appro-
priate number and sampling locations of SPG analysis should
be determined by the State or EPA depending on local con-
ditions, and significant deviations from the "normal" range
must be reported to the State or EPA and corrective actions
taken immediately. The proposed regulations also limit the
use of chlorine dioxide as a primary disinfectant to not
exceed 1 mg/1 because of possible adverse effects of by-
products and would not permit the use of chloramines as primary
disinfectants but only for maintenance of a distribution system
residual.

-------
                          -  750 -

EPA has also proposed that water systems subject to significant
raw water contamination from pollutions-related synthetic organic
chemicals initiate a program to study, design and construct
facilities utilizing granular  activated carbon or equivalent
technology^ to minimize human exposure to those contaminants.

In conclusion, I think the United States has come a long way
in providing safe drinking water to her population. The Interim
Primary Regulations and the proposed trihalomethane regulations
are an important beginning. Our Revised Regulations, presently
under consideration, will supplement our present efforts and
begin to deal with contaminants where long-term impacts are
beginning to be understood  (especially carcinogens). However,
in the future I see our attention being focused on the estab-
lishment of regulations that require specific water treatment
for supplies that are shown to be at risk from specific
contaminants. These regulations could specify disinfection,
maximum contaminant levels for individual contaminants,
coagulation and filtration, or other treatments when warranted.
Such an approach is not only cost-effective, but in reality
a sensible way to provide the public health protection needed.

I want to thank you for the opportunity to speak with you today
and I look forward to working with you in the future as we
together, in cooperation, provide safe drinking water to the
citizens of our respective countries.

-------
                          -  751  -
Y. Kott (Israel)
The surplus of water in the northern area of the country
and severe scarcity in the south, the utilization of over
95 percent of the water potential in the country and the
utilization of about 85 percent of the water for agricul-
tural purposes, caused an intervention of the government
to pass a law which nationalized all water resources from
individual property to the public.

This law enabled the national water company to pump win-
ter surplus water from lake Kinnereth and recharge it
into sandy aquifer in the coastal plane through a dual-
purpose well. During storage, water quality changed and
the number of coliform bacteria rose up to 1O /1OO ml;
turbidity was very high. Water quality improved at a long
storage or due to continuous pumping. The various quality
problems that have arisen by these operations caused the
Ministry of Health to request a committee that was wor-
king on a new law for water quality to establish a qua-
lity criterion for such water. Indeed, the Israel Drinking
Water Standard published in 1974 states at clause 14
that when drinking water is pumped from a dual-purpose
well it should be examined and found to be free of fecal
coliforms, streptococci and salmonella bacteria. In addi-
tion no more than two coliforms in 1OO ml would be allowed
and the water must be disinfected. As much as we .know, no
other country has as yet established this criterion in
its water standard.

As already mentioned above, most of the water is used for
agricultural purposes. The need for water has created a

-------
                         - -752 - .
situation in which the farmers are ready to utilize va- '<•
rious qualities of wastewater. It is estimated that re-
use of wastewater will reach in the near future to over
120 million cubic meters. The importance of quality ver-
sus type of crop growth has brought to create new cri-
teria for purified wastewater quality.

The irrigation with treated wastewater will have four
quality levels in which the unlimited one will have to
be 80 percentite of coliform, equal or less than 12, fe-
cal coliform, fifty percentile 2.2/100 ml. Residual chlo-
rine of 0.5 mg/1. It is mandatory that the water will
have to pass sand filtration. On the other hand, group A
which will allow irrigation of industrial crop like cotton,
dry fodder, seeds etc. will allow BOD of 6O mg/1, suspen-
ded solids up to 5O mg/1.

The mechanism of utilizing most of the water resources
and dividing the quality for each type of crop  will main-
tain the true balance between needs and capability. It is
thought that combination of utilisation of all water re-
sources for the various needs will postpone the need for
water desalination from big plants which are much more
expensive.

-------
                           -  753  -
G. Muller-  (P.'R.G.)
In Germany the addition of chlorine to drinking water is
regulated by two laws, i.e. the Foodstuffs'and Necessities
Law and the Federal Epidemics Law.  On the basis of these two
laws statutory decrees have been laid down,  one of these
being a decree concerning the treatment of drinking water
specifying the maximum amount of chlorine that can be added
to drinking water.  The amount in question-is 0.3 mg Cl-/!.
In times of emergency or disaster this value can be increased
to 0.6 mg C12/1.  On the other hand, the Drinking Water Decree
on the authorization of the Federal Epidemics Law lays down
the minimum amount of chlorine when this is to be used as a
disinfectant in drinking water.  The chlorine content in
drinking water leaving the works must be 0.1 mg Cl^/l-  In
combination with this, chlorinated water ex works must not exceed
a standard colony count value of 20 ml.  The purpose of this
combination is to guarantee the actual effectiveness of the
chlorination.

The minimum and maximum \alues prescribed in the decrees for
chlorine in drinking water show that in the Federal Republic
the chlorine level permitted by law lies within very narrow
limits.

Experience to date has shown that this chlorine content may
be just sufficient to destroy pathogens entering the drinking
water network by penetration of waste water or river water,
especially since the organic substance flowing in at the same
time consumes a large proportion of the chlorine before the
disinfection occurs.  This has been demonstrated time and
again in recent decades in cases of typhoid and paratyphoid-B
epidemics or epidemics due to other Salmonella species.  The
so-called safety chlorination normally makes it possible to
reduce elevated colony counts, but is rarely sufficient for

-------
                          - 75.4 -


a reliable destruction of  the pathogens that penetrate the ..
supply network via short circuits, cross connections, and
sucking back.
In Germany there are many public water supplies working on
ground water that cannot be faulted from a bacteriological
point of view, where the water is not chlorinated on leaving
the works because bacterial proliferation, detectable by
elevated colony counts, is simply not observed during the
distribution or storage of the water.

-------
                          - 755.-
J.A. Myhrstad  (Norway)
The drinking water regulations in Norway are based on
the Health Act, which is administered by the Ministry
of Social Affairs.
According to the regulations, potable water should be
hygienic, safe, the water source should be protected
from microbiological and chemical pollution, and  the wa-
ter treated in a proper way.

The regulations also state that waterworks supplying
between  100 and 1000 persons are subject to approval by
the local Boards of Health, and the greater works are
subject  to approval by the National Institute of  Public
Health.  Since there is no detailed information on how to
proceed  in order to achieve a wholesome potable water,
it is the responsibility of these authorities to  consi-
der the  necessary actions to be taken.

The waterworks can be given the right to expropriate
land in  the catchment areas and put restrictions  on the
existing and future activities, according to the  Water
Resources Act, in order to protect the water sources from
pollution.

The Water Pollution Act is an important law which covers
all forms of water pollution, and it contains a general
prohibition, unless permission is granted, against most
activities liable to cause water pollution. This  law is
administered by the Ministry of Environment.

According to the drinking water regulations,  the water-
works are responsible for the water quality control. This
control  is very often carried out in close cooperation

-------
                          - 756 -

                  *     -..,.. 	  . ,  .   ,   . i ,   -i	,	,-,	
with the local 'Board of Health and National Institute of
Public Health.

In addition to the analysis,of raw water,  treated water
and tap water samples, plant control and weekly reports
concerning communicable diseases in each community are
integrated in the control'procedure.

Surface water is by far the most important water source
in Norway, at least for waterworks supplying more than
100 persons. About 95 % of the population  supplied by
these waterworks use surface water. The most important
quality problems are related to the. low pH-values of the
surface water  (down to a pH-value of 3.4), the very soft
water (total hardness usually below 5-1O mg CaO/1) and
the coloured water (e.g. colours in the range of 3O-6O
mg Pt/1).

Treatment problems are mainly related to low water tem-
peratures in the winter time, which influence the coagu-
lation process, and the coloured water being extensively
used without adequate treatment, interfering with the
chlorination process.

Chlorine is still the most important disinfectant used,
but breakpoint chlorination is never achieved owing to
the very small concentrations of ammonia  (usually well
below O.1 ppm ammonia - nitrogen). There is a tendency
to replace chlorine by ultraviolet irradiation. Chlorine
dioxide will not be used until the health  effect has been
evaluated.
Examinations carried out have revealed high concentra-
tions of trihalomethanes caused by chlorination of co-
loured water.

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                       - - 757 -

Other problems are related to the fact that many water-
works do not treat the water in the proper way, and that
waterworks equipment, usually imported from abroad, is
produced in countries with water qualities differing very
much from the Norwegian water quality. One example is
the asbestos-cement pipes without internal coating. Ca
is extracted from the pipe material resulting in pH-va-
lues as high as 11.5. These pH-values increase the dis-
solution of heavy metals from taps made of brass alloys
and solders. The asbestos-fibre content in the water
will also be influenced.

We have no specific or detailed regulations concerning
water quality, but we have recommendations. Some orga-
nic micro-pollutants are included in the recommenda-
tions;  however, trihalomethanes are not included for
the time being.

The present drinking water regulations will be revised
in the near future.

An expert group has recently been appointed by the Royal
Norwegian Council for Scientific and Industrial Research
to evaluate the need for research in the field of drin-
king water supply.

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                           758 -
G „ 
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                          - 759 -

city screening tests are already studied at. this moment
and will be a subject of thorough research in the near
future.

On safety ground it is recommended that water should ge-
nerally be treated by physical and biological processes
to the greatest possible extent and that chemical oxidants
should not be used more often than strictly necessary.
However, the use of chlorine as a disinfectant will be re-
placed by other means only after their effects have been
thoroughly studied and have proved to be fully acceptable.

An important subject which will be studied is the question of
whether the risks of chemical oxydation are great enough
to outweigh.large claims on surface areas which are used
at this moment, e.g. in dune infiltration. A deeper insight
is necessary to evaluate the long-term risks to human
health. Epidemiological studies are carried out at this
moment for this purpose.

From the viewpoint of risks to human health standards or
criteria for organic compounds in tapwater or raw water
destined for the drinking water supply can only be set
as interim regulations until more insight is obtained.
Other aspects which are considered are future structural
plans to obtain a reliable quality of potable water. The
protection of raw water sources can be performed by water
sanitation programs and protection ,of the underground
against industrial pollution. Storage of pretreated water
should be in places where aging of the water can occur,
with avoiding further pollution.
Criteria for organics in drinking water will possibly
be proposed not only for individual compounds but also
for group- and sum parameters and possibly by a series
of mutagenicity screening tests.

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                          _, 760 -
T. Stenstrorn  (Sweden)
Public water supplies in Sweden should, according to the
law, be investigated bacteriologically with a frequency
of at least once a week for large water supplies to once
every third month for ground water supplies serving less
than 4OOO people. Private water supplies, usually hotels
etc., should be checked in the'same way. The chemical
quality of the water is tested once a year. The samples
are taken at the water-works.

Water- is classified as not suitable if the total number
of coliforms (MPN-method, 35°G, 48" h) is more than 1O/1OO
ml. If this number is 1-9 or i'f the total plate count
(22 C, 48 h) is more than 1OO the water is classified as
less suitable.  The total plate" count at 22°C is a valuable
indicator of general contamination and of the efficiency
of the water treatment and disinfection.

Private wells are also tested for fecal coliforms (MPN
44 C, 48 h). Such water is classified as unsuitable if the
number of fecal coliforms is-more than 1O or if the total
number of coliforms is more' than 5OO.

The chemical quality control is'relatively similar to that
recommended by WHO. Heavy metals and organic micropollutants
are not analysed routinely. The only parameter used routi-
nely for organic substances is permanganate consumption. A
large survey for trihalometanes has, however, shown that
most water supplies have concentrations below 1O pg/1 of
the trihalomethanes at the consumer * s tap.

In only two cases out of 144 the concentration was above
1OO yg/1.

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                          -• 761  --

The Swedish regulations from 1967 and 1970 should-=be'revi-
sed in a few years. Recommendations for chemical and bac-
teriological monitoring of the water quality in distribu-
tion systems and at the consumer's tap will be published
early in 1979. The purpose of this is
1)  to extend the monitoring of parameters of importance
   for possible health effects to the consumer's tap, and
2)  to provide data for making operational decisions  in
   order to avoid problems like .taste, odor or colour cau-
   sed by corrosion o,r. bacteriological activity in the net-
   work. For the bacteriological monitoring the membrane
   method will probably be accepted. It is not so today.

Of the water from public water supplies in Sweden 53% is
surface water. 21% is infiltrated water usually from sandy
ridges deposited during the glacial period. 26% is ground
water without infiltration. About 15% of the population is
not connected to public wa,ter-supplies. They generally use
ground water. Restrictions on the use of polluted surface
waters as water supplies are not .considered necessary in
Sweden.

8O% of the surface water is treated with chemical floccula-
tion and filtration. Half of this water is also treated by
slow sand filtration, for example by the water-works in
Stockholm. Only rapid or slow sand filtration is used for
6 and 9% of the water, respectively.

The most common disinfectant i-s chlorine although chlorine
dioxide is used in some water-works such as Gothenburg.
Breakpoint chlorination as an oxidation step is not  used.
With very few exceptions the ammonia concentrations  are
below O.5 mg/1 and do not cause any problems except  that
nitrite up to a few tenth of a mg/1 may be produced  in the
distribution system. Many water-works, e.g. Stockholm, add

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                         - 762 -

ammonia to get chloramines. Ozon is used only at two
water-works.

Much of the Swedish research on drinking water problems is
now focused on the deterioration of water quality during
distribution. New treatment methods such as activated
carbon filtration will also be studied. The philosophy is
that water at the consumer's tap should be not only whole-
some but of a high quality with regard to taste, odor and
appearance.

Another problem, which should be studied, is the treatment
at small water-works of water containing e>g~. high concen-
trations of iron and manganese,.

Except for some local areas, Sweden has no shortage of raw
waters of a high quality. This is to a large extent due to
strict regulations on the discharge of domestic and indu-
strial sewage water. About 70% of the Swedish population
living in cities and villages with more than 2OO persons
are now connected to waste-water-works with biological as
well as chemical treatment. Another 26% has mechanical and
biological treatment (usually active sludge). Only 3% has
only mechanical treatment which is not accepted according
to the law. An increasing number of waste-water-works is
also supplied with a final filtration.

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                          - 763 -
P. Toft  (Canada)
Canada has a federal system of government and consists
of 10 provinces and two territories. The distribution
of powers between the provinces and the federal govern-
ment is stated in the British North America Act of 1867.
Although this Act does not deal specifically with water.
resources, and therefore drinking water supply, judicial
interpretation over the years has resulted in a situ-
ation where there is a shared federal-provincial res-
ponsibility.

Under the Act, the ownership of natural resources, in-
cluding water, is vested in the provinces. Thus the pro-
vinces have the primary authority to legislate in the
area of municipal water supply.

Under their respective Health Acts the provincial de-
partments of health also have the power to control con-
ditions which constitute a threat to human health.

At the federal level, responsibility is assigned to the
Department of National Health and Welfare to investigate
and conduct programmes related to public health. For ex-
ample, research and investigation into the health aspects
of drinking water are undertaken with a view to public
health protection. In carrying out.such activities the
Department is required to co-ordinate its efforts with
those of the appropriate provincial authorities.

In Canada, drinking water is defined as a food and there-
fore in theory it could be regulated under the Food and
Drugs Act. Although this has been done for bottled (mi-
neral and spring)  waters,  standards  have not been ex-

-------
                          -  764 -


tended to i'tap ' wa'ter-' owing "to" the major role traditionally
assumed by the provinces.

Generally the provinces play the lead role in ensuring
an adequate and safe supply of drinking water whereas the
Federal government provides leadership in ensuring ade-
quate standards for drinking water quality especially to
protect human health.     •'•...

There are a few specific cases where the Federal govern-
ment is solely responsible for drinking water. These in-
                                    <•
elude administering potable water regulations for all
common carriers (transportation crossing Canadian Inter-
provincial and International borders), and on Canadian
coastal shipping vessels, and the provision of potable
water in the Territories, Indian, reservations and mili-
tary bases.
Standards

With the exception of a few provinces which specify that
municipal water supplies must contain a certain minimal
level of chlorine, there are no legally enforceable stan-
dards for drinking water supplies in Canada. Rather,
there are guidelines for potable water quality and strict
control over the design and operation of treatment plants,
The federal government plays a lead role in establishing
drinking water guidelines.
Revision of the Canadian Drinking Water Guidelines

It was in  1968 that the first Canadian Drinking Water
Document was brought out by a joint committee comprising

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                          -.765 -


the Canadian Public Hearth Association Drinking <.Water>
Standards Committee and  a federally-convened Advisory
Committee on Public Health Engineering. The latter in-
cluded representatives of the Federal and Provincial
Departments of Health, several universities and Canadian
water pollution and water resource control agencies.
Provincial governments,  in turn,, .have adapted these
"guidelines" to suit  their own particular situation.
A joint federal-provincial group led by the Department
of National Health and Welfare.is, currently revising
the drinking water standards  published in 1968.
                               * U.S. GOVERNMENT TOWING OFHCE: 1979-281-147/122

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