vvEPA
        United States
        Environmental Protection
        Agency	
           Prevention, Pesticides
           And Toxic Substances
           (7508W)
EPA738-R-97-013
December 1997
Reregistration
Eligibility Decision (RED)

THIOBENCARB

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                 United States
                 Environmental Protection
                 Agency '.	
Prevention,' Pesticides
And Toxic Substances
(75O8W)	
EPA-738-F-97-013
September 1997
                 R.E.D.   FACTS
                 Thiobencarb
     Pesticide    *  All pesticides sold or distributed in the United States must be
Reregistration   registered by EPA, based on scientific studies showing that they can be used
                 without posing unreasonable risks to people or the environment. Because of
                 advances in scientific knowledge, the law requires that pesticides which
                 were first registered before November 1, 1984, be reregistered to ensure
                 that they meet today's more stringent standards.
                     In evaluating pesticides for reregistration, EPA obtains and reviews a
                 complete set of studies from pesticide producers, describing the human
        •         health and .environmental effects of each pesticide. To implement provisions
                 of the Food Quality Protection Act of 1996, EPA considers the special
                 sensitivity of infants and children to pesticides, as well as aggregate
                 exposure of the public to pesticide residues from all sources, and the
                 cumulative effects of pesticides and other compounds with common
                 mechanisms of toxicity. The Agency develops any mitigation measures or
                 regulatory controls needed to effectively reduce each pesticide's risks. EPA
                 then reregisters pesticides that meet the safety standard of the FQPA and can
                 be used without posing unreasonable risks to human health or the
                 environment.
                     When a pesticide is eligible for reregistration, EPA explains the basis
                 for its decision in a Reregistration Eligibility Decision (RED) document.
                 This fact sheet summarizes the.information in the RED document for
                 reregistration case 2665, Thiobencarb.

   Use Profile    .   Thiobencarb is a systemic, pre-emergence herbicide that acts by
                 inhibiting shoots of emerging seedlings. It is used to control grasses, sedge
                 and broadleaf weeds in food crops such as rice (represents 95 % of use),
                 lettuce, celery, and endive.  Formulations include a liquid and a granular.
                 Thiobencarb may be applied using ground spray equipment or by aircraft.

   Regulatory       Thiobencarb was first registered for use on rice in 1982. In 1991,
       History   thiobencarb was issued regional tolerances for use on celery, endives, and
                 lettuce in the State of Florida.

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                                    Currently, there are five products containing thiobencarb registered
                              under Section 3 of theFederal insecticide, Fungicide, and Rodenticide Act.
                              They consist of one technical (manufacturing use) product containing 97.4%
                          ;    active ingredient, two emulsifiable concentrate end-use products each
                              containing 84^0% active ingredient, arid two granular end-use products each
                              containing 10.0% active ingredient.

           Human Health  "Toxicity
             Assessment        In studies using laboratory animals, thiobencarb generally has been
                              shown to be of low acute toxicity^ It is slightly toxic by the oral and dermal
                              route and has been placed in category m for these effects. It is practically
                              non-toxic by the, inhalation routeTarid for eye irritation, and has been placed
                              in Toxicity Category W (ffieiowestbf four categories) fo^
                              Thiobencarb also tested negative for mutagenicity.
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                              Dietary Exposure
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                                    People may be exposed to residues of thiobencarb through the diet.
                              Tolerances have been established for celery, lettuce, endive (escarole), rice
                              (grain and straw), and for animal commodities including milk and eggs,  and
                              the fat, meat, and meat by-products of cattle, hogs, poultry, sheep, goats
                              and horses (please see 40  CFR18()r401(a) arid (b)).  EPA has reassessed the
                              thiobencarb tolerances and found that they are acceptable.
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                              Occupational and Residential Exposure
                                    Exposure to homeowners is not expected since there are no residential
                              uses.  Based on current use patterns, handlers (mixers, loaders, and
                              applicators) may be exposed to thiobencarbi during and after normal
                              agricultural use.
                                    The risks calculated from intermediate-term exposures to thiobencarb
                              indicate that risks from exposures to granular formulations (loading and
                              applying)  are lower than those from exposures to the liquid formulation/
                               spray applications. For'line granular fbrmulatioris, theMOEs exceed 100 for
                              all scenarios (except loading to support aerial application) with the addition
                               of personal protectivelequipriienE For many of the liquid formulation/spray
                               application scenarios, the MOEs do  not reach 100, even with engineering
-  :   ,.'     i:;:: '       "   "';,;:; •,  controls.	;
                                    Granular Formulations: The Agency believes the risks resulting from
                               intermediate-term exposures to the granular formulation are overestimated
                               due to the use of a 60.2 percent dermal absorption value for the granular
                               scenarios.  In general, dermal absorption of granular formulations has been
                               found to be significantly lower than for liquid formulations. Therefore, the
                               Agency has determined that risks to"handlersi of granular formulations will
                               be adequately mitigated with the addition of personal protective equipment.

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      Liquid Formulations: The Agency believes that risks resulting from
 intermediate-term exposures to persons handling liquid formulations are
 overestimated due to limitations with the hazard identification and the dose-
 response assessment for the intermediate-term endpoint, particularly in light
 of the, absence of serious effects to these target organs in either the
 subchronic neurotoxicity or rat chronic feeding study, which suggest the
 lack of a deleterious response to thiobencarb by the kidney and/or liver. The
 Agency believes that risks to handlers using liquid formulations will be
 adequately mitigated with the used of engineering controls and personal
 protective equipment.
 Human Risk Assessment
      Thiobencarb generally is of low acute toxicity, and has been classified
 in Group D (not classifiable as to human carcinogenicity). Food crop uses
 are registered including rice, lettuce, endive and escarole.  However,
 dietary exposure to thiobencarb residues in foods is extremely low, as is the
 cancer risk posed to the general population.
      Of greater concern is the risk posed to thiobencarb handlers,
 particularly mixers/loaders/applicators,  and field workers who come into
 contact with treated crops following application of this'pesticide.  Exposure
 and risk to workers will be mitigated by the use of PPE required by the
 WPS, supplemented by engineering controls as required by this RED.
      For post-application workers, since no dislodgeable foliar residue
 studies or concurrent dermal samples were submitted to the EPA for
 thiobencarb to measure postapplication reentry, a rough surrogate •
 postapplication assessment was performed as a default.  Only the short-term
 endpoint (25 mg/kg/day) was used for the risk assessment, since EPA does
 not anticipate that intermediate-term exposures (i.e., 7 days or more of
 exposure) are likely to occur for post-application workers for these crops in
 early-stage development. EPA assumed in the surrogate assessment that
 dermal absorption would be significantly lower that the 60.2 percent used in
 the handler assessment, since dermal exposure would be to dry residues. -
 The surrogate postapplication risk assessment indicated that at all application
 rates  (i.e., 4-8 pounds active ingredient per acre), risks would be acceptable
 to post-application workers entering treated areas to perform tasks such as
 scouting, thinning, or hoeing, provided entry is postponed for 24 hours
 following application.
 FQPA Considerations
      The FQPA of 1996 amended the FFDCA by setting a new safety
 standard for the establishment of tolerances  and directs the EPA to consider
 available information concerning the susceptibility of infants and children to
pesticide residues in food.  Based on the review of available data and the
 absence of incident or epidemiological data for thiobencarb, an additional
 safety factor for the protection of children is riot necessary.  The FQPA also

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                                 requires EPA to consider aggregate exposure to the pesticide residue,
                                 including all anticipated dietary exposure and other exposures for which
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                                 there is reliable information, as well as cumulative effects from the pesticide
                                 and pther substances tiiaf have a common mechanism of foxicity.
                                      EPA has assessed the dietary risk posed by thiobencarb.  In assessing
                                 chronic dietary risk ,  EPA estimates that thiobencarb residues in food
                                 sources account for _^. 42.9 % of the RfD, and include the highest-at-risk
                                 subgroup, non-nursing infants.  In drinking water thiobencarb residues
                                 account for 0.29% of the RfD.  Thus, the aggregate exposures from all
                                 sources of thiobencarb (in this case, only dietary and drinking water
                                 exposures are relevant) account for 43.2% of the RfD.  Therefore, the
                                 Agency concludesittiat aggregate ris^
                                 from tfiiobencarb uses are not of concern.
                           	si    ,  " "' ,i ' ii'iir	„  	|  ","  i, ' Hi1,                               •• ••          '   '         • •
                                      EPX estimates that thiobencarb residues in the diet of infants and
                                 children account for 42.9% of the RfD (29.5 for children 1-5) and residues
                                 k drinking water account for 0.29%  of the RfD.  Thus the aggregate
                                 exposure from alt sources of thiobencarb account for 43.2 %  of the RfD for
                                 infants ami children^  Therefore, the Agency concludes that aggregate risks
                                 for infants and children resulting from uses of thiobencarb are not of
                                 concern.
	nil,
             Environmental   Environmental Fate
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                Ass                   Thiobencarb is slightly persistent k water, generally not very mobile,
                                 tends to bmd to soil organic matter, and doesn't desorb. Generally,
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                                 thiobencarb is Stable to degradation by hydrolysis and is stable under
                                 anaerobic aquatic conditions. Thlobencaib meets the persistence and
                                 mobility triggers for classification as a restricted-use chemical for ground-
                                 water concerns, but not the detections triggers.  The Agency believes that
                                 ground water concerns do not warrant use restrictions. Furthermore, due to
                                 the binding nature of the active kgredient, any thiobencarb that may reach
                                 surface water will be predominantly bound with suspended solids and
                                 sedinie^s.  Standard coagulatim
 ":'< '"!'  :'     ?'".• •   '. •  ;;   ;'*:?! 'i{. '^6xp1^e^ to remove mosVof1 Me suspen^e^ solids and sedments from tfe  ";
                                 water^ thereby rembvkg most of the potential risk of thiobencarb k
                                 drinkkg water.
  •] '••         ,:;"        •    :";,;;  :  ECOlOQical Eff6CtS	
                                       Use of liquid formulations of thiobencarb pose some acute risk to
                                 mammals.  The acute risk to birds is mkimal.  Use of liquid formulations
                                 pose a high chronic risk to birds and mammals.
                                       Use of' thiobencarb on.rice Jin tite southeast US poses a Mgh risk of
                                 chronic effects to freshwater and estuarke aquatic kvertebrates, includkg
                                 shrimp and mollusks  There is also likely a high risk of chronic effects to
                                 fish,  but additional data are needed to confirm this. This use of thiobencarb

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                     also poses a high risk of acute effects, to fish and aquatic invertebrates in
                     certain high-exposure situations.  Additionally, use of thiobencarb on rice in
                     California poses a risk of causing chronic effects to aquatic organisms in the
                 -    smaller drains and waterways, but not in the larger rivers. Thiobencarb use
                     poses minimal risk of acute effects to fish and aquatic invertebrates.
                     Minimal risk of both acute and chronic effects is expected for all estuarine
                     organisms in California.  Spray drift from aerial application of liquid
                     thiobencarb on rice poses a high risk to nontarget terrestrial and semiaquatic
                     plants.  Drift of granular thiobencarb and spraying of liquid thiobencarb
                     applied with ground equipment pose minimal risk to these plants.  All uses
                     of thiobencarb on rice may pose a risk of killing emerging seedlings of
                     aquatic plants, especially aquatic grasses. Use of thiobencarb on rice may
                     pose a risk to aquatic algae in the southeast US, and in smaller drains and
                     waterways in California.
                          Use of thiobencarb On celery, lettuce, and endive in Florida poses a
                     high risk of causing chronic effects to fish, ifreshwater invertebrates, and
                     estuarine invertebrates, including shrimp.  Additionally, this use poses a
                     high risk of causing acute effects to freshwater and estuarine invertebrates,
                     including oysters  and shrimp.  Use of thiobencarb on celery, lettuce, and
                     endive in Florida poses a high risk to terrestrial plants, semiaquatic plants,
                     and algae.  It may also pose a risk to emerging seedlings of vascular aquatic
                     plants.
                     Environmental Risk  Characterization
                          Of particular concern to the Agency is the high risk of chronic effects
                     to fish and freshwater invertebrates, including shrimp and mollusks, and the
                     high risk of causing acute effects to freshwater and invertebrates, and the
                     water quality risks posed by the chemical.

Risk Mitigation        To lessen worker risk, and ecological and water quality risks posed by
                     thiobencarb, FJPA is requiring the following mitigation measures from
                     registrants of thiobencarb-containing products.
                     To protect handlers :
                     •     For liquid formulations:  mixers and loaders must used closed systems
                          in addition to wearing a chemical-resistant apron, chemical-resistant
                          gloves, long-sleeve  shirt, long pants, shoes, and socks.  Applicators
                          and flaggers must use enclosed cabs or cockpits and wear long-sleeve
                          shirt, long pants, shoes, and socks.
                     •     For granular formulations:  loaders must wear a chemical-resistant
                          apron, chemical-resistant gloves, long-sleeve shirt, long pants, shoes,
                          and socks.  Applicators and flaggers must wear chemical-resistant
                          gloves, long-sleeve shirt, long pants, shoes, and socks.

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                'Sill .1.. ' !, t'l1' •:',	.','W	Niiii '^"V;  :'•	mr-'s
                     To protect workers:
                     ป     A restricted-entry interval of 24 hours is being imposed.  Early entry
                           workers must wear coveralls, chemical-resistant gloves, shoes, and
                           socks.
                      To protect non-target organisms:
                     •      ^
                            and Texas.  In Louisiana, thiobencarb application will not be allowed
                            south of the Intracoastal Waterway.  In Texas, thiobencarb
                            application will not be allowed within two miles inland from the
                            shorelines of Galveston Bay, and not within two miles of Matagorda
                      •     Include label warnings preventing application to rice fields with
                            catfish/crayfish farming, and preventing application to rice fields
                            adjacent to catfish or crayfish ponds.
                      •     Where weather conditions'permit, it is required that flood waters not
                            be released within 14 days.
                      •     Require that thiobencarb not be applied within 24 hours of rainfall,
                            or when heavy rain is expected to occur within 24 hours.
                      •     Require that thiobencarb not be mixed/loaded or otherwise handled
                            within 100 feet of aquatic habitat.
                      •     Continue existing label warnings addressing environmental hazards,
                            such as restricting application aerially within one mile of the St.
                            Francis Floqdway where the Fat Pocketbook Pearly Mussel is known
                            to occur. ''Comparable wanungs  would be appropriate where use on
                            ric^
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                      •     jfefk K^.ttef.PilAr^l. ISIP8688 m ™ฎ Fa3i ^f 1998 thlobencarb use
                            on leafy vegetables in Florida based on the results of the currently
                            ongoing^environmental monitoring study for muck soils in Florida
                            from the U.S. CJeological Survey's National Water Quality
                            Assessment Program (NAWQA).
  ,Sh ,	  v '   ' >"W':'\  ••'         ' I          I            I        i       ,  .'-: .  '. . .             -   "
Additional Data         EPAis requiring the following additional generic studies for
         Required   thiobencarb to confirm its regulatory assessments and conclusions:
                      •    : Dermal Penetration Study [GLN85-2J;
                      •     Life-Cycle" Freshwater Fish Study [GLN 72-5J;
                      •     Avian Subacute Toxicity Study [GLN 71-2(b)];
                    '  •     Avian Reproduction Study [GLN 71-4(b)J;
                      •     Seedling Emergence Testing Study [GLN 123-1 (a)],-
                            The Agency also is requiring product-specific data including product
                      chemistry and acute toxicity studies, revised Confidential Statements of
                      Formula (CSFs), and revised labeling for reregistration.

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Product Labeling
Changes Required
       AH thiobencarb end-use products must comply with EPA's current
 pesticide product labeling requirements and with the following requirements:
 Labeling Requirements for Manufacturing-Use Products
       To remain in compliance with FEFRA, manufacturing use product
 (MP) labeling must be revised to comply with all current EPA regulations,
 PR Notices and applicable policies.  The MP labeling must bear the
 following statement under Directions for Use:
       "Only for formulation into an Herbicide for the following use(s):rice
      weed control in California, Louisiana, Texas, Mississippi, Missouri
      and Arkansas, and lettuce, endive and celery weed control in
      Florida."
      An MP registrant may,  at his/her discretion, add one of the following
 statements to an MP label under "Directions for Use" to .permit the
 reformulation of the product for a specific use or all additional uses
 supported by a formulator or user group:
      (a)     "This product may be used to formulate products for
              specific use(s) not listed on the MP, label if the
              formulator, user group, or grower has complied with
              U.S. EPA submission requirements regarding support
              of suchuse(s)."
      (b)     "This product may be used to formulate products for
             any additional use(s) not listed on the MP label if the
             formulator, user group, or grower has complied with
             U.S. EPA submission requirements regarding support
             of suchuse(s)."
 Labeling Requirements for End-Use Products
             The labels and labeling of all products must comply with
      EPA's current regulations and requirements as specified in 40 CFR
      ง156.10 and other applicable notices.
 PPE and Engineering Control  Requirements for Pesticide Handlers
      For sole-active-ingredient end-use products that contain thiobencarb:
      •      Revise the product labeling to adopt the handler personal
             protective equipment/engineering control requirements set
             forth in this section.
      •      Remove any conflicting PPE requirements on the current
             labeling.
      For multiple-active-ingredient end-use products that contain
thiobencarb:                                               ,
      •      Compare the handler personal protective
             equipment/engineering control requirements set forth in this
             section to  the (requirements on the current labeling.

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             Retain the more protective requirements. (For guidance on
             which requirements are considered more protective, see PR
Active-Ingredient Specific Engineering Control Requirements
  , ' '  ' . jin'lil"1!1" ' '!,!,„,' ' 1J1" '!i ''Hi":"'!1 i11,!1! i" •!,! !:S,i|i M,! ,! iซ ! "A IS! ,i:','il '!!::!' ' ,11.;, i. '' '!,,' WllnftlM '.i'l".', ii! ..... i!!1" ..ijl'J"' **,!" 'i, .'. ''  , ,.„, i   ,ป' ,h
      EPA is estabiishing active-ingredient specific engineering controls for
some occupational uses of thiobencarb end-use products.
      For liquid formulations:
            M      ii       i          I  i    ,,",.'
             "Mixers and loaders are required to use closed
             systems. The closed system must be used in a manner
             that meets  the requirements listed in the Worker
             Protection Standard (WPS) for agricultural pesticides
             (40 CFR 170.240(d)(4)."
             "Applicators and flaggers are required to use enclosed cabs or
             enclosed cockpits. The closed system must be used in a
             manner that meets the requirements listed in the Worker
             Protection Standard (WPS) for agricultural pesticides (40
             CFRl70.240(d)(5-6)."
Active-Ingredient Specific Personal Protective Equipment Requirements
                   II   II    II   I '  I I  I I I     ,. 'I,', 1" ;'  : ,''    .    •     "
             EPA is establishing active-ingredient specific personal
      protective equipment requirements for all occupational uses of
      thiobencarb end-use products.
      For liquid formulations:
    'I  , II .„„ " i i, ....... I  •*• ,.,•.!• ....... ....... ......... ........ ..... , , ........ .....   • • „ ............. ••  / , ' '  "       '
                "In addition to using closed systems, mixers and loaders
  : , .  ;>.,;>;, • • ....... TOjist wear : .....................
             ^ long-sleeved shirt and long pants,
             —chemical-resistant gloves*,
             —sOcks plus shoes, and
             —chemical-resistant apron. "
             "Applicators and flaggers using enclosed cabs or cockpits ,
      \ ...... ;;  i must wear:  ............................................
             — long-sleeved shirt and long pants, and
             —socks plus shoes. "
             "For other handling activities and in case of a spill or other  .
             emergency exposure, handlers must wear:
             —coveralls over long-sleeved shirt and long pants,
             —chemical-resistalat gloves*,
             —chemical-resistant footwear, and
             —chemical-resistant apron when cleaning equipment. "

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              *For the glove statement, use the statement established for
              thiobencarb through the instructions in Supplement Three of
              PR Notice 93-7.
      For granular formulations:
              "Applicators and other handlers must wear:
              — long-sleeved shirt and long pants,
              —chemical-resistant gloves*,
              — shoes plus socks
              —chemical-resistant apron when loading formulation into,
              equipment or cleaning equipment."
              *For the glove statement, use the statement established for
              thiobencarb through the instructions in Supplement Three of
              PR Notice 93-7.
a.    Entry Restrictions
      For sole-active-ingredient end-use products that contain thiobencarb:
      •       Revise the product labeling to adopt the entry restrictions set
              forth in this section.
      •.      Remove any conflicting entry restrictions on the current
              labeling.
      For multiple-active-ingredient end-use products that contain
thiobencarb:
      •       Compare the entry restrictions set forth in this section to the
              entry restrictions on the current labeling.
      •       Retain the more protective restrictions. (A specific time
              period in hours or days is considered more protective than
              "sprays have dried" or "dusts have settled.")
Restricted-entry interval:  A 24-hour restricted-entry interval (RET) is
required for uses within the scope of the WPS on all thiobencarb end-use
products.
Early-Entry Personal Protective Equipment rPPElr The PPE required for
early entry is:
      - coveralls,                             ,
      - chemical-resistant gloves, and
      - shoes plus socks.                                  :


   ,   Other Labeling Requirements
      The Agency is requiring the following labeling statements to be
located on all end-use products containing thiobencarb:

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                i.  Application Restrictions

                "Do not apply this product in a way that will contact workers or other
                persons, either directly or indirectly or through drift.  Only protected
                handlers may be in the area duringapplication."
                "Do not apply this product south of the Intracoastal Waterway in
    ;';;=|   ',  '.,,:;; ^touisiana.",,	,	,	,	.,  i	,,'	
                "Do not apply this product within two (2) miles from the shorelines of
                Matagorda Bay in Texas."
                "Do not apply this product within two (2) miles from the shorelines of
                Galveston Bay in Texas."
                "Do not apply this product to rice fields with catfish/crayfish
                farming."
                'Tpo not apply this product on rice fields adjacent to catfish or crayfish
                ponds."
                'When applying to rice fields, do not release permanent flood water
                within 14-days of application of this product (where weather
    ri ''",	"."permits)."	  '	
                "Avoid application of this product within 24 hours of rainfall, or when
                he^
    j:i|,|'ii|!i| ', |,  'i ,* i '„;: ,  ',',1'::;' , $jf''9 I '^"{['•^"^'.'^^'.^^	f:'v i",'!"!11!"1'!! "	'^^/^firf!111'1'''1!!''!! I!!':,':' Wi'1'1' Jl! liSi'liil''1!^    "i   '','''' 	      "   ,    " .  :,•,•'
                "Do not mix/load orol&erwise> handle this product within 100 feet of
                aquatic habitat."

                ii.  User Safety Requirements
    1'i'n i .as \  
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 "Users should remove PPE immediately after handling this product.
 Wash the outside of gloves before removing.  As soon as possible,
 wish thoroughly and change into clean clothing."

 iv.  Spray Drift Labeling

 The following language must be placed on each product label that can
 be applied aerially:
 Avoiding spray drift at the application site is the responsibility of the
 applicator. The interaction of many equipment-and-w,eather-related
 factors determine the potential for spray drift.  The applicator and the
 grower are responsible for considering all these factors when making
 decisions.
 The following Aerial Drift Reduction Advisory Information must be
 followed to avoid off-target drift movement from aerial applications to
 agricultural field crops. These requirements do not apply to forestry
 applications, public health uses or to applications using dry
 formulations.                :
 1.      The distance of the outer most nozzles on the boom must not
        exceed 3/4 the length of the wingspan or rotor.
 2.      Nozzles must always point backward parallel with the air
        stream and never be pointed downwards more than 45
        degrees.                    '
 Where states have more stringent regulations, they should be
 observed.
 It is recommended that the applicator should be familiar with and take
 into account the information covered in the Aerial Drift Reduction
 Advisory Information.       ,
 The following aerial drift reduction advisory information must be
 contained in the product labeling:
 [This section is advisory in nature and does not supersede the
 mandatory label requirements.]     .                     ,
 INFORMATION ON DROPLET SIZE:  The most effective way to
 reduce drift potential is to apply large droplets.  The best drift
 management strategy is to  apply the largest droplets that provide
 sufficient coverage and control.  Applying larger droplets reduces
 drift potential, but will not prevent drift if applications are made
improperly, or under unfavorable environmental conditions (see
Wind, Temperature and Humidity, and Temperature Inversions).
                 11

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" 31
           CONTROLLING DROPLET SIZE
           •       Volume - Use high flow rate nozzles to apply the highest
           practical spray volume.  Nozzles with higher rated flows produce
           larger droplets.
           •       Pressure - Do not exceed the nozzle manufacturer's
           recommended pressures.  For many nozzle types lower pressure
           produces larger droplets. When higher flow rates are needed, use
           higher flow rate nozzles instead of increasing pressure.
           •'  •',"	;";' |,t; , "...W.L'ifi'i iMi'iV-v'JW^f'd il.iff hi-* ซ1	'Kii.t'i! ! • •,.. 	   • ',.'      	••"•:'','.
           •       Number of nozzles - Use the minimum number of nozzles
          •'•>:*!'::(	•^•\v^,?.t''.iV*\-'- >->;<:f*u	;;>:''  •'•!	M,,i>.'i:. •  • '    "-        .    • •, :•: ,•
           that provide uniform coverage.
           '$ .  .    Nozzle Orientation - Orienting nozzles so that the spray is
           released parallel to the airstream produces larger droplets than other
           orientations and is the recommended practice. Significant deflection
           from horizontal will reduce droplet size and increase drift potential.
            i'   i        i iji'i   i, 'i i 11     	i     iji 'i  .•. '•,>,••• > ' .  - ,    • •
           •       Nozzle Type - Use a nozzle type that is designed for the
           intended application. Witn most nozzle types, narrower spray angles
           produce largerdroplets.  Consider using low-drift nozzles.  Solid
           stream nozzles oriented straight back produce the largest droplets and
           the lowest drift.
           •       Maintenance of Nozzles - periodic inspection and subsequent
           replacement of nozzles to ensure proper chemical application is
BOOM LENGTH: For some use patterns, reducing the effective
boom length to less than 3/4 of the wingspan or rotor length may
further reduce drift without reducing swath width.
APPLICATION HEIGHT: Applications should not be made at a
height greater than 10 feet above the top of the largest plants unless a
greater height is required for aircraft safety.  Making applications at
the lowest height that is safe reduces exposure of droplets to
evaporation and wind.
SWATH ADJUSTMENT:  When applications are made with a
crosswind, the  swath will be displaced downward. Therefore, on the
tip arid downwind edges of the field, the applicator must compensate
for this displacement by adjusting the path of the aircraft upwind.
Swath adjustment distance should increase, with increasing drift
potential (higher wind, smaller drops, etc.)
WIND:  Drift potential is lowest between wind speeds of 2-10 mph.
However, many factors, including droplet size and equipment type
determine drift potential at any given speed.  Application should be
avoided below  2 mph due to variable wind direction and high
inversion potential. 'NOTE:'' Local terrain can influence wind
                             12

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 Regulatory
 Conclusion
   For More
Information
      patterns. Every applicator should be familiar with local wind patterns
     , and how they affect spray drift.
      TEMPERATURE AND HUMIDITY:  When making applications in
      low relative humidity, set up equipment to produce larger droplets to
      compensate for evaporation.  Droplet evaporation is most severe when
      conditions are both hot and dry.                      •,
      TEMPERATURE INVERSIONS: Applications should not occur
      during a temperature inversion because drift potential is high.
      Temperature inversions restrict vertical air mixing, which causes
      small suspended droplets to remain in a concentrated cloud.  This
      cloud can move in unpredictable directions due to the light variable
      winds common during inversions.  Temperature inversions are
      characterized by increasing temperatures with altitude and are
      common on nights with limited cloud cover and light to no wind.
      They begin to form as the sun sets and often continue into the
      morning. Their presence can be indicated by ground fog; however, if
      fog is not present, inversions can also be identified by the movement
      of smoke from a ground source or an aircraft smoke generator.
      Smoke that layers and moves laterally in a concentrated cloud (under
      low wind conditions) indicates an inversion, while Asmoke that moves
      upward and rapidly dissipates indicates good vertical air mixing.
      SENSITIVE AREAS:  The pesticide should only be applied when the
      potential for drift to adjacent  sensitive areas (e.g. residential areas,
      bodies of water, known habitat for threatened or endangered species,
      non-target crops) is minimal (e.g, when wind is blowing away from
      the sensitive areas).

      The use of currently registered products containing thiobencarb in
accordance with approved labeling will not pose unreasonable risks or
adverse effects to  humans or the environment.  Therefore, all uses of these
products are eligible for reregistration.
      Thiobencarb products will be reregistered once the required product-
specific data, revised Confidential Statements of Formula, and revised
labeling are received and accepted by EPA.

      EPA is requesting public comments on the Reregistration Eligibility
Decision (RED) document for thiobencarb during a 60-day time period, as
announced in a Notice of Availability published in the Federal Register. To
obtain a copy of the RED document or to submit written comments, please
contact the Pesticide Docket, Public Information and Records  Integrity
Branch, Information Resources and Services Division (7502C), Office of
Pesticide Programs (OPP), US EPA, Washington, DC 20460, telephone
703-305-5805.                                   '
                                      13

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             a	:" f ,.j,'
             •it  ':
             ii V ",:
I?!,,, ' 11'
                            .'if!	II,
                            at;:
                            VIX
                            it;,!!
       JBlectronic copies of the RED and this Set sheet are available on the
" ,;'';' ii'V i,,;, '"•;";! I1!]1 • ^ iilji'iiiiii • pm. l!j!:if,|j,f, [ |. f'"< (/MI M ™  •!	.ill1 .41 '^'\. !:i,ป;ปi • ii. u ',11: lit .fli.	\"V:'~ ,\4\,\ ft • i j'UHlln f: '''i ;,';• „" .!••.,'    '  i1   •       ,   ' , • i, '•
 Internet.  Seehttp://www.epa.goy/KEDs.
       Printed copies of the RED and fact sheet can be obtained from EPA's
         j^^
              ", PO"BOX"42419,  Cincinnati^ OH 45242-2419, telephone 1-
 806-490-9198; fax5i3-489:&$fL
:•';',;!! •' „ i: 1;E!IIS '•',:,^! •&^ฃM^ i:; !,!:'ii!a'S', ../I iill	I'iS,,"^ i',;fi ^	s,-,":. ,'.,.•   :''':•   •  ' '' v '• -"';
      ? JfoJIpwing the comment period, the thiobencarb RED document also
 will be available .from the National Te^hdcjl^^pimation Service (NTIS),
 5285 Port Royal Road,  Springfield, VA 22161, telephone 703-487-4650.
„ ''Jil1 •; i	, ,,1|1!1!!ll!"i .;;l!1,l;2!ป V ii'*, 'il i1;,^, • 'ti1'iS!i!!!.!i|\!Jii,!;i11l!i, ii|;,;|1ilil!.1"'1! *" ,1 ''' "Jv1'',!;;;!*:!:!:,'!	II1. ^y1'!!11'1:1 ,:lh.! 'I i,. ''I;1'.:1; ''• " ••'  '-  ',  "' i;"'   ''.  '"        '.','•'
       Foi•more mformation about EPA's pesticide reregistration program,
 	\.C'::;,,':'.'i,-S'.: ff,<.,i,fi't!,Wtiปi	-'Witt*	'.'i	v.	6*nif.
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      *.          UNITED STATES ENVIRONMENTAL PROTECTION AGENCY
                                 WASHINGTON, D.C. 20460
                                      MAY-27 1998
                                                                              OFFICE OF
                                                                        PREVENTION, PESTICIDES AND
                                                                           TOXIC SUBSTANCES
The official approved date for Thiobencarb is September 30, 1997 and the issued date is May
27, 1998.

           RecycletVRecyclable . Printed with Vegetable OH Based Inks on 100% Recycled Paper (40% Postconsumer)

-------

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                    UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

                                      WASHINGTON, D.C. 20460
                                                                           OFFICE OF
                                                                     PREVENTION, PESTICIDES
                                                                      AND TOXIC SUBSTANCES
 CERTIFIED MATT.
                                           3 0 1997
 Dear Registrant:                                     '•-.-•

       I am pleased to announce that the Environmental Protection Agency has completed its
 reregistration eligibility review and decisions on the pesticide chemical case thiobencarb which
 includes the active ingredient S-[(4-chlorophenyl)methyl]diethyl carbamothioate. The enclosed
 Reregistration Eligibility Decision (RED), which was approved on September 30, 1997
 contains the Agency's evaluation of the data base of the chemical, its conclusions of the
 potential human health and environmental risks  of the current product uses, and its decisions
 and conditions under which these uses and products will be eligible for reregistration. The
 RED includes the data and labeling requirements for products for reregistration. It also
 includes requirements for additional data (generic) on the active ingredient to confirm the risk
 assessments.

       To assist you with a proper response, read the enclosed document entitled "Summary
 of Instructions for Responding to the RED." This summary also refers to other enclosed
 documents which include further instructions. You must follow all instructions and submit
 complete and timely responses.  The first set of required responses is due 90 days from the
 receipt of this letter.  The second set of required responses is due 8 months from the
 receipt of this letter.  Complete and timely responses will avoid the Agency taking the
 enforcement action of suspension against your products.                                 '

       Please note that the Food Quality Protection Act of 1996 (FQPA) became.effective on
August 3, 1996, amending portions of both the pesticide law (FIFRA) and the food and drug
law (FFDCA).  This RED takes into account, to the extent currently possible, the new safety
standard set by FQPA for establishing and reassessing tolerances.  However, it should be
noted that in continuing to make reregistration determinations during the early stages of FQPA
implementation, EPA recognizes that'll will be necessary to make decisions relating to FQPA
before the implementation process is complete.   In making these early case-by-case decisions,
EPA does not intend to set broad precedents for  the application of FQPA.  Rather, these early
determinations will be made on a case-by-case basis and will not bind EPA as it proceeds with
further policy development and any rulemaMng that may be required.

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       If EPA determines, as a result of this later implementation process, that any of the
determinations described in this RED are no longer appropriate, the Agency will pursue
whatever action may be appropriate, including but not limited to reconsideration of any
portion of this RED.

       If you have questions on the product specific data requirements or wish to meet with
the Agency, please contact the Special Review and Reregistration Division representative C.P.
Moran at (703) 308-8009.  Please address any questions on required generic data to the
Special Review and Reregistration Division representative Patrick Dobak at (703)308-8180.

                                                     Sincerely,


          -                                        V7
:;:  •••.'.-.   '                                  'tfot
                                                     I/ois A. Rossi, Director
                                                     Special Review and
                                                      Reregistration Division
Enclosures

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               SUMMARY OF INSTRUCTIONS FOR RESPONDING TO
              THE REREGISTRATTON ELIGIBILITY DECISION (RED)

 1  DATA CALL-IN (PCD OR "90-DAY RESPONSE"-If generic data am
 reregistration; a DCI letter will be enclosed describing such data.  If product specific data
 are required, a DCI letter .will be enclosed listing such requirements.  If both generic and
 product specific data are required, a combined Generic and Product Specific DCI letter will
 be enclosed describing suph data. However, if you are an end-use product registrant only and
 have been granted a generic data exemption (GDE) by EPA, you are being sent only the
 product specific response forms (2 forms) with the RED. Registrants responsible for generic
 data are being sent response forms for both generic and product specific data requirements (4
 forms).  You must submit the appropriate response forms (following the instructions
 provided) within 90 days of the receipt of this RED/DCI letter; otherwise, your product
 may be suspended.

 2-. TIME EXTENSIONS AND DATA WATVER REQTTESTS-Nn time extension requests
 will be granted for the 90-day response. Time extension requests may be submitted only with
 respect to actual data .submissions.  Requests for time extensions for product specific data
 should be submitted in the 90-day response. Requests for data waivers must be submitted as
 part of the 90-day response. All data waiver and time extension requests must be accompanied
 by a full justification.  All waivers  and time extensions must be granted by EPA in order to go
 into'effect.        •         .   •;

 3  APPLICATION FOR REKEGISTRATION OR "8-MONTH RESPONSE"-Vmi mud-
 submit the following items for each product within eight months of the date of this letter
 (RED issuance date).
       a-  Application for Reregistratinn (RPA Form gyzn-i)  Use only an original
application form.  Mark it "Application for Reregistration. " Send your Application for
Reregistration (along with the other forms listed in b-e below) to the address listed in item 5.
                                      •          •          i.   - '                    .._
       b.  Five copies of draft labeling which complies with the RED and current regulations
and requirements. Only make labeling changes which are required by the RED and current
regulations (40 CFR 156.10) and policies. Submit any other amendments (such as formulation
changes, or labeling changes not related to reregistxation) separately.  You may, but are not
required to, delete uses which the RED says are ineligible for reregistration.  For further
labeling guidance, refer to the labeling section of the EPA publication "General Information
on Applying for Registration in the U.S., Second Edition, August 1992" (available from the
National Technical Information Service, publication #PB92-221811; telephone number 703-
487-4650).                        ....'•                        ,

  , ,    c-   Generic or Product Specific Data. Submit all data in a format which complies
with PR Notice 86-5, and/or submit citations of data already submitted and, give the EPA
identifier (MRID) numbers.  Before citing these studies, you must make sure that they meet
the Agency's acceptance criteria (attached to the DCI).

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      d. Two copies of the Confidential Statement of Formula (CSF) for each basic and
each alternate formulation.  The labeling and CSF which you submit for each product must
comply with P.R. Notice 91-2 by declaring the active ingredient as the nominal
ephcentratipn.  You have two options for submitting a CSF: (1) accept the standard certified
limits (see 40 CFR ง158.175)' or (2) provide> certifieci."HmTts mat are supported by the analysis
df five batches.  If you choose me second option, you must submit or cite the data for the five
batches along with a certification statement as described in 40 CFR ง158.175(e).  A copy of
the CSF is enclosed; follow the^instructions on its back.
:.".• .".;' .e,' dert^^tionWitV^e^ect'tp jPata Compensation Requirements. " Complete and'
sign Effi'jfom 1570-31	
    	SNTS M' jjajjgrojj^
pertaining to" the	coh'tenVofliiell^^	t6"tnel"a'H3r"ess shown in'the Federal
Register Notice which announces the availability of this RED.

$.' JBgHERELTO SEND PRODUCT SPECIFIC PCI RESPONSES (90-DAY) AND
APPLICATIONS FOR REREGISTRATION (8-MONTH RESPONSES)

งv tJ.S. Mall:
      Document IPrO'Cessing Desk (
      Office of "Pesticide Prpgi^is' '(754)
      EPA, 4Q1 MSt. S.W.
      Washington, D.C. 20460-0001
 !,!i,i    •' ,i  , •,.'
By .express!
       Document Processing Desk (RED-SRRD-PRB)
       Office of Pesticide Programs (7504C)
       Rpom266A,( Crystal Mall 2     ,,„;,," ...... ;
       i92i Jefferson Davis &wy.
6. EPAiSJSE\DDBWS— EPA will screen all submissions for completeness; those which are not
complete will be returned with a request for corrections. EPA will try to respond to data
waiver and time extension ."requests wiffiin '."60 days.  EPA will also try to respond to all 8-
nipnth subrnissipns wim a fi^                                             the RED
has been issued.

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^REGISTRATION ELIGIBILITY DECISION




           TfflOBENCARB






               LISTB




              CASE 2665

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                        TABLE OF CONTENTS
TfflOBENCARB REREGISTRATION ELIGIBILITY DECISION TEAM ..,...:..... i

ABSTRACT. ....... , .  . . .		'...... .''. . v

I.     INTRODUCTION .  .  .	 . . .	 . 1

H.    CASE OVERVIEW	  		         1
      A.    Chemical Overview	 . ;		\
      B.    Use Profile	 2
      C.    Estimated Usagfc of Pesticide	.'...: 3
      D.    Regulatory History	        4

      SCIENCE ASSESSMENT		  . .	.4
      A.    Physical Chemistry Assessment	 4
      B.    Human Health Assessment	5
           1.     Toxicology Assessment	 5
                 a.    Acute Toxicity	  ........... 5
                 b.    Subchronic Toxicity	8
                 c.    Chronic Toxicity		8
                 d.    Carcinogenicity	........"	9
                 e.    Developmental Toxicity	 9
                 f.     Reproductive Toxicity  .............. ;	10
                 g.    Mutagenicity	10
                 h.    Metabolism  .	  11
                 i.     Neurotoxicity	  11
               J.     Endocrine Disrupter Effects  .	13
           2.     Toxicological Endpoints for Risk Assessment .	13
                 a.    Reference Dose (RfD)	'.......  13
                 b.    Carcinogenic Classification	 .  13
                 d.   Dietary Exposure from Drinking Water	20
                 e.     Occupational Exposure	 . .	  21
                 f.     Aggregate Exposure	  24
           3.     Risk Assessment	 .  24
                 a.    Dietary Risk ...... . . ;....:.	  25
                 b.    Occupational Risk	  28
                 c.     Short-Term Residential Risk	 . . . .  33
                 d.    Cumulative Risk Assessments		33
           4.     Risk Characterization .	  .	33
                 a.    Hazard Identification and Dose-Response	  33
                 b.    Occupational Exposure . . . .	34
     C.    Environmental Assessment .	             . .  35

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81	;; ,!;	11	
II; V'
    1;,., :i!i- i
    I':!'', if'1' "
      '
             1.     Ecological Toxicity Data	 . 35
                   a.     Toxicity to Terrestrial Animals  . .	35
                   b.     Toxicity to Aquatic Animals  	38
                   c.     Toxicity to Plants  	49
             2.     Environmental Fate	51
                   a.     Environmental Fate Assessment	, . .	  .. , 51
                   b.     Environmental Fate and Transport	53
   11   , ' •      ' I    ,      II  II  I	       II      *       '      •            • .:
                   d.     Water Resources		60
             3.     Exposure and Risk Characterization	 64
                   a.     Ecological Exposure and Risk Characterization  	64
             4.     Risk Characterization	86
                   a.     Extent of Use	86
                   b.     Summaryof Risk Assessment  . . .	 . 87
wi; •'.  ."  ;>-i-  ,. '.'i'itii i:;'\"c.     impacts to  Water'Resources  . . 1' ....'..'	\  . . 88
:|l,;,;,;  ' :4,,,;'-  •', •; 'IS /"j': ;;i
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      B.    End-Use Products	  112
            1.    Additional Product-Specific Data Requirements	: . .  .  112
            2.    Labeling Requirements for End-Use Products  ............  112
                 a.    PPE and Engineering Control Requirements for Pesticide
                       Handlers	,	113
            3.    Products Intended Primarily for Occupational Use . . .... .-.  .  113
            4.    Active-Ingredient Specific Personal Protective Equipment
                 Requirements	•....;.,	  113
            5.    Determining PPE Labeling Requirements for End-use Products
                 Containing This Active Ingredient	 .   	114
            6.    Placement in Labeling	  115
            7,    Entry Restrictions	        115
            8.    Products Intended Primarily for Occupational Use .........  115
      C.    Spray Drift Labeling	    117
      D.    Existing Stocks	  119

VI.   APPENDICES	 . .	 . .	.  . .		.       121
      APPENDIX A.    Table of Use Patterns Subject to Reregistration  ......  122
      APPENDIX B.    Table of the Generic Data Requirements and Studies Used to
                       Make the Reregistration Decision	.'.  129
      APPENDIX C.    Citations Considered to be Part of the Data Base Supporting
                       the Reregistration of Thiobencarb  . .	  135
      APPENDIX D.    Generic Data Call-in	  .	  146
            Attachment  1.     Chemical Status Sheet	,.	 .  165
            Attachment  2.     Generic DCI Response Forms Inserts (Form A) plus
                             Instructions .	  166
            Attachment  3.     Requirements Status and Registrants'Response Forms
                             Inserts (FormB) plus Instructions ...........  170
            Attachment  4.     List of Registrant^) sent this DCI (Insert)   ....;  177
      APPENDIX E.  .  Product Specific Data Call-in .  . . ] .	 .  179
            Attachment  1.     Chemical Status Sheets .	  193
            Attachment  2.     Product Specific Data Call-In Response Forms (Form
                             A inserts) Plus Instructions	 . .  195
            Attachment  3.     Product Specific Requirement Status and Registrant's
                             Response Forms (Form B inserts) and Instructions
                              •••.,;...........	'. , 197
            Attachment  4.     EPA Batching of End-Use Products for Meeting Data
                             Requirements for Reregistration	204
            Attachment  5.     List of Registrants) sent this DCI (Insert)  .....  207
            Attachment  6.     Cost Share, Data Compensation Forms, Confidential
                             Statement of Formula Form and Instructions ...  208
      APPENDIX F.    List of Available Related Documents	215

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THIOBENCARB REREGISTRATION ELIGIBILITY DECISION TEAM

Office of Pesticide Programs:

Biological and Economic Analysis Assessment
James Saulmbn
Yuen-Shaung Ng
Edward Brandt
Neil Anderson
Michael Yanchulis
Herbicide and Insecticide Branch
Science Information and Analysis Branch
Economic Analysis Branch
Herbicide and Insecticide Branch
Herbicide and Insecticide Branch
Environmental Fate and Effects Risk Assessment
NickMastrota
James Breithaupt
David Wells
Robert Hitch

Health Effects Risk Assessment

Paul Lewis
Stephen Dapson
David Miller
Brian Steinwand
Alan Nielson

Registration Support Risk Assessment

Daniel Kenny
Joanne Miller

Risk Management
Environmental Risk Branch JJ
Environmental Risk Branch n
Environmental Risk Branch U
Environmental Risk Branch U
Risk Characterization and Analysis Branch
Toxicology Branch U
Chemistry and Exposure Branch U
Chemistry and Exposure Branch IJ
Reregistration Branch U
Fungicide-Herbicide Branch
Fungicide-Herbicide Branch
Dennis Deziel, Team Leader
Reregistration Branch I

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ft I.1    I,'
hi '    	I!'  ,"'

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             GLOSSARY OF  TERMS AND ABBREVIATIONS
ADI
AE
ad.
ARC
CAS
CI
CNS   •
CSF
DFR
DRES
DWEL

EEC

EP
EPA
FAO/WHO
FDA
FEFRA
FFDCA
FQPA
FOB
GLC
GM
GRAS
HA

HDT
LEL
LOG
LOD
LOEL
MATC
MCLG

MBYP
/Ug/L
mg/L
MOE
MP
MPI
MRDD
 Acceptable Daily Intake.  A now defunct term for reference dose (RfD).
 Acid Equivalent                                                   .
 Active Ingredient                                                                     _
 Anticipated Residue Contribution
 Chemical Abstracts Service
 Cation                             ,
 Central Nervous System         '                ,
 Confidential Statement of Formula
 Dislodgeable Foliar Residue
 Dietary -Risk Evaluation System                                       •
 Drinking Water Equivalent Level (DWEL) The DWEL represents a medium specific (i.e. drinking
 water) lifetime exposure at which adverse, non carcinogenic health effects are not anticipated to occur.
 Estimated Environmental Concentration.  The estimated pesticide concentration in an environment,
 such as a terrestrial ecosystem.                             .
 End-Use Product
 U.S. Environmental Protection Agency   '
 Food and Agriculture Organization/World Health Organization   .               •       '
 Food and Drug Administration                             . . .
 Federal Insecticide, Fungicide, and Rodenticide Act
, Federal Food, Drug, and Cosmetic Act
 Food Quality Protection Act
 Functional Observation Battery              " .  ,
 Gas Liquid Chromatography  •
 Geometric Mean
 Generally Recognized as Safe as Designated by FDA
 Health Advisory (HA).  The HA .values are used as informal guidance to municipalities and other
 organizations when emergency spills or contamination situations occur.
 Highest Dose Tested
 Median Lethal Concentration.  A statistically derived concentration of a substance that can be expected
 to cause death in 50 % of test animals. It is usually expressed as the weight of substance per weight
 or volume of water, air or feed, e.g., mg/1, mg/kg or ppm.
 Median Lethal Dose.  A statistically derived single dose that can be expected to cause death in 50%
 of the test animals when administered by the route indicated (oral, dermal, inhalation).  It is expressed
 as a weight of substance .per unit weight of animal, e.g., mg/kg.
 Lethal Dose-low. Lowest Dose at which lethality occurs,
 Lowest Effect Level                           ,                  ,
 Level of Concern                            '
 Limit of Detection
 Lowest Observed Effect Level . '
 Maximum Acceptable Toxicant Concentration
 Maximum Contaminant Level Goal (MCLG)  The MCLG is used by the Agency to regulate
 contaminants in drinking water under the Safe Drinking Water Act.
 Meat By-Product
 Micrograms Per Gram                                                                 >
 Micrograms per liter                                                     •
 Milligrams Per Liter
 Margin of Exposure
 Manufacturing-Use Product
 Maximum Permissible Intake                                    .
 Master Record Identification (number). EPA's system of recording and tracking studies submitted.
                                                 Ill

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                            GLOSSARY OF  TERMS  AND ABBREVIATIONS
ll
               NPDES
               NOEL
               NOAEL
               OP
               !OPP
               ?a
               fADt
               '.|AG
               PHED
               :|>pm
               Q".
               RBC
               RED
 'It*
 "SLN
 TC
 TD
 fEP
..TOAI
               TjMCRC
               torr
                 Not Applicable
                 If o Qbseryable Effect Concentration
                 National Pollutant Discharge Elimination System
                 No Observed Effect Level
                 No Observed Adverse Effect Level
                 prganophosphate
                 pascal, the pressure exerted by a force of one newton acting on an area of one square meter.
                 Provisional Acceptable Daily Intake
                 Pgstipide Assessment Guideline
                 pesticide Analytical Method
                 Pesticide Handler's Exposure Data
                 i,:':iWill l!l1	'M" ,;'. i1:",,,, v,	v1,,, ;.r!:" , "iii'ir1:!1!-,  	     	  - -	  , 	 n  ,
                 Preharvest Interval
                 W	i MIJ .•" ... ij'.'.j, ,ป	i!t	'-in	 ' . .  •'•:.llt,' l! '	• v ". ::•:.<. -in	ri . I,;".1,1"' •                  •
                 Parts Per Billion
                 Personal Protective Equipment
                 Parts Per Million
                 Pesticide Registration Notice
                 The Carcinogenic Potential of a Compound, Quantified by the EPA's Cancer Risk Model
                 Red Blood Cell
                 Reregistration Eligibility Decision
                 Restricted Entry Interval
Jegistration Standard
Restricted Use Pesticide [[[
Special Local Need (Registrations Under Section 24(c) of Jb'JUKKA)
Toxic Concentration. The concentration at which a substance produces a toxic effect.
Toxic Dose. The dose at which a substance produces a toxic effect.
Typical 'End-Use'Product'1 "
Technical Grade Active Ingredient
Thin Layer Chromatography
                /ps
                 A unit of pressure needed to support a column of mercury 1 mm high under standard conditions.
                 Wettable Powder ..... """  ,"','', ......... ''„","' ^  ', .  '. ..... , ....... '„'.'.

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ABSTRACT
              This Reregistration Eligibility  Document (RED)  addresses the  eligibility  for
       reregistration of pesticide products containing the active ingredient thiobencarb  S-((4-
       chlorophenyl)methyl) diethylcarbamothioate.   This  decision includes  a comprehensive
       reassessment of the  required target data and the use patterns of currently registered
      .products.                              L. .   .                        .             ;

              Thiobencarb is a thiocarbamate herbicide that is applied primarily to rice (95%),
       as  well as to lettuce, celery and endives  to control  grasses and  broadleaf weeds.
       Thiobencarb is sold in the United States by Valent U.S.A. Corporation. There are five
       registered products.

              Thiobencarb was first registered for use on rice in 1982.  In 1991, thiobencarb was
       issued regional tolerances for use on celery, endives, and lettuce in the state of Florida.
       Thiobencarb is applied as a liquid and granular (California only) using fixed-wing aircraft,
       helicopter, granular tractor-drawn spreader, and groundboom sprayer.

              The Agency has concluded that all uses, as prescribed in this document, will not
       cause unreasonable risks to humans or the environment and therefore, all products are
       eligible for reregistration.  However, to mitigate risks for  certain uses, the Agency has
       determined that the following actions must be taken in regard to thiobencarb:

       1.      Prohibit application of thiobencarb  south of the Intracoastal Waterway in
              Louisiana, based on the high  risk of chronic  effects  to fish and freshwater
              invertebrates,  including shrimp and mollusks, and the high risk of causing acute
              effects to freshwater and invertebrates. .This affects approximately 10,000 acres of
              rice, less than 5 percent of total rice acreage in Louisiana.  ,

       2.      Prohibit application of  thiobencarb  within two (2) miles  inland from the
              shorelines of  Galveston and Matagorda Bays in Texas, based on the high risk
              of chronic  effects to fish and freshwater invertebrates, including shrimp and
              mollusks, and the high risk of causing acute effects to freshwater and  invertebrates.
              This affects approximately  2,000-5,000 acres of rice, about 1 percent of total rice
              acreage in Texas..

       3.      Institute a 14-Day holding period following thiobencarb application to rice
              fields, where  weather permits, due to the risks associated with thiobencarb and
              thiobencarb sulfoxide residues in runoff and receiving w.aters.

       4.      Continue use of thiobencarb on vegetable crops  (currently celery, endives and
              lettuce)  and  rice in Florida,  a  decision which will be reassessed with the
              completion of ongoing environmental monitoring studies in Florida (Fall 1998).

-------
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                           The generic data base supporting the reregistration of thiobencarb for the above
                    eligible uses has been reviewed _ and _ determined^                          Additional
                    data foi occupational exposure, ecological effects and environmental fate  are being
                    required to confirm the Agency's risk assessment and conclusions.
                         *    in  • i, I,  '            ii            ill   i    <     ; "   • '";,„  i, • • •  i •
                           In establishing or reassessing tolerances, the Food Quality and Protection Act of
                    1996 (FQPA) requires the Agency to consider aggregate exposures to pesticide residues,
                    including all anticipated dietary exposures and other exposures  for which there is reliable
                    informay,pn? as wel]l as the > potential for cumulative > effect from a  pesticide and  other
                    compounds with a common '^i^hanisrn "of toxicity ! The FQPA furmer directs the Agency
                    to consider potential for increased susceptibility of infants and children to the toxic effects
                    of pesticide residue.   The  Agency considers  the  appropriateness  of an  additional
                    Uncertainly factor J which can" be applied in situations' where available data indicate infants
                    and children may have an increased sensitivity to the pesticide.  In general, the data base
                    for thiobencarb does not jndicate a potential for increased lexicological sensitivity from
                    either pre- or post-natal exposures. No developmental toxicity was observed in either the
                    rat  or  the  rabbit developmental toxicity  studies, nor was there evidence in the two-
                    genef ation reproduction study of develop mental or reproductive toxicity at dose levels
                   'BejIpW  tSst, in wfrigti, parental toxicity  was  observed.   Therefore, .the Agency  has
                   ."(determined that an additional uncertainty factor is not warranted.
                    "',  "'; ' • "SI : ',!'';':" ......... .•.,'••' ..... >"lft:'}f 'ft *'ป''*•'); 'ft ...... lWfiijMM ........ .Ari';.:.(. "in, ;;if,;, .,-1 I, 'ii, x ,:, (.•(' 'o'.at.V'l 'j' p ......... ', J" IE1! ,'":,"!"'. •  "''     ' '.
                    ,„",>,""•'  'i! ''I' ..... ii;^:..^i^^ ...... ^i^^mii^m^^immti^m:^:'   • ;  .•.••.  •    '  ••   ,  ;
                          JBgfQre reregistering the products containing thiobencarb, the Agency is requiring
                    that product specific data, revised Confidential Statements of Formula (CSF) and revised
                    labeling Be submitted within eight months of the issuance of this  document.  These data
                    include product chemistry for each registration and acute toxicity testing.  After reviewing
                    these data and any revised labels and finding them acceptable in accordance with Section
                    3(c)(5) of FIFRA, the Agency will reregister a product.  Those products which contain
                    other active ingredients will  be eligible for reregistration only  when the other active
                    ingredients are determined to be eligible for reregistration.
lit : i:
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                                                         VI

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I.
INTRODUCTION
       In 1988, the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) was amended
to accelerate the reregistration of products with active ingredients registered prior to November
1, 1984. The amended Act provides a schedule for the reregistration process to be completed in
nine years. There are five phases to the reregistration process. The, first four phases of the process
focus on identification of data requirements to support the reregistration of an active ingredient
and the generation and submission  of data to fulfill the requirements. The fifth phase is a review
by the U.S. Environmental Protection Agency (referred to as "the Agency") of all data submitted
to support reregistration.    .  ^                  ,                  .

       FIFRA Section  4(g)(2)(A)  states that in  Phase 5 "the Administrator shall determine
whether pesticides containing such active ingredient are eligible for reregistration" before calling
in data on products  and either reregistering products or taking "other appropriate regulatory
action." Thus, reregistration involves a thorough review of the scientific data base underlying a
pesticide's registration. The purpose of the Agency's review is to reassess the potential hazards
arising from the currently registered uses of the pesticide; to determine the need for additional.
data on health and environmental effects; and to determine whether the pesticide meets the "no
unreasonable adverse effects" criterion of FIFRA.          .                .

       This document presents the Agency's decision regarding the reregistration eligibility of
the registered uses of thiobencarb. The document consists of six sections. Section  I  is the
introduction. Section n describes thiobencarb,  its uses, data requirements and regulatory history.
Section IE discusses the human health and environmental, assessment based on the data available
to the Agency. Section IV presents the reregistration decision.for thiobencarb  . Section V
discusses the reregistration requirements for thiobencarb . Finally, Section VI is the Appendices
which support this Reregistration Eligibility Decision. Additional details concerning the Agency's
review of applicable  data .are available on request.

H.    CASE OVERVIEW

       A.     Chemical Overview

              The following  active ingredient is covered  by this Reregistration Eligibility
           .  Decision:
              Common Name:

              Chemical Name:

              Chemical Family:

              CAS Registry Number:
                                  Thiobencarb

                                  S-((4-chlorophenyl)methyl)diethylcarbamothioate

                                  Thiocarbamate         '

                                  28249-77-6                      '

-------
    "I  '\ "'MSI!
       OPP Chemical Code:
       Empirical Formula:
                           108401
                           C12H16CINOS
       Trade and Other Names:   Bolero
                                  Bencarb
       "'*".'.  .     	.''  ".   ' '' '  -"	Saturn
       ";':       ••    ;  ::	 	"  :	B"3pl5
                                  IMC 3950
B.
Basic Manufacturer:

Use Profile
                                  Valent U.S.A. Corporation
       The following is information on the currently registered uses with an overview of
use sites and application methods.  A detailed table of the use of thiobencarb is in
Appendix A.

       For thiobencarb:

       Type of Pesticide:   Systemic Herbicide

       Mode of Action:    Inhibits shoots of emerging seedlings
 :  • , :  ' ! li            'hi       j i  '   !        "      I      ,.',"''.      '•  .''''',',
       TJse Sites:           Rice, lettuce, celery, and endive
       Target Plants:
                    Broadleaf weeds, grasses, and sedge
       Specific types:  Hemp sesbania, redstem, purple ammannia, teaweed; southern
       naiad, ducksalad, mud plantain, fall panicgrass, texas millet, hurrahgrass, annual
       bluegrass, foxtail, johnsongrass, jointvetch, sicklepod, watergrass, gulf cockspur,
       goOsegrass, Uttle barley, sprangletop, red rice, panicum, paragrass,  broadleaf
       signalgrass, cheat, downy brome, crabgrass, junglerice, barnyardgrass, spikerush,
       fimbristylis  mUiaceae,   roughseed  bulrush,  horned  beakrush, waterwort,
       mexicanweed, texasweed, woolly croton, redstem filaree, Carolina foxtail, pitted
       morningglory, mustard,  shepherdspurse, flatsedge, smallflower umbrellaplant,
       gobsewee'H, J cMckweed,''	'''larnbsquarters,	dayflower,   echpta,  dandelion,
       r|pri|ingglory, pigweed, purslane,  warerplantain,  arrowhead,  alligatorweed,
       redroot pigweed, redstem, sprangletop, cheat, crabgrass, spikerush, fimbristylis
       jiiiliaceae,  mustard,    lambsquarters,  dayflower,  pickerelweed,  buttercup,
       wlterhyssop,  false pimpernel, redweed, and marestail
                                                    i          •                • • I
        III  i          i          , >   i           '      i      '         '        ;     •••;!
       Formulation Types Registered:

-------
             TECHNICAL GRADE
           ,  ACTIVE INGREDIENT:
             END USE PRODUCT:
             GRANULAR:

       Method and Rates of Application:
Liquid 97.40%
Emulsifiable Concentrate
10.00%
84.00%
           .  Equipment -   Aircraft;  Boom sprayer;  Ground; Low pressure ground
                           sprayer; Low volume ground sprayer;  Sprayer.

             Method -     Soil treatment; Spray; Water application.

             Rate -        Rice         2 to 41bs a.L/acre
                           Lettuce       61bs  a.i./acre         '
                           Endive       61bs a.i./acre          -  -  ..  '
                           Celery       6 to 81bs a.i./acre

             Timing -      Early-ppstemergence;  Post-emergence;  Post-transplant;
                           Preemergenee; Seed bed.

       Use Practice Limitations:                                          .

       Do not apply directly to water (Liquid formulation).  Do riot apply through any
type of irrigation system. Do not discharge effluent containing this pesticide into sewage
systems without notifying the sewage treatment plant authority (POTW). Do not discharge
effluent containing .this product into lakes, streams, ponds, estuaries, oceans,' or public
water.  (NPDES license restriction) For terrestrial uses, do not apply directly to water or
to areas where surface water is present or to intertidal areas below the mean high water
mark.                             ,

       Site/Application Limitations: For celery, endives (escarole), and lettuce provide
for a 60-day preharvest interval                       , '                  .

C,     Estimated Usage of Pesticide

       This section summarizes  the best estimates available for the pesticide uses of
thiobencarb.  These estimates are derived from a variety of published and proprietary
sources available to the Agency.  The data, reported on an aggregate and site (crop) basis,
reflect annual fluctuations in use patterns as well as the variability in using data from
various information sources.                                           •

       Approximately  1.22  million pounds ,of  thiobencarb  are applied  annually  on
465,000 acres (460,000 of which is rice).  Based on data from the National Agricultural
Pesticide Impact Assessment Project, four states — Arkansas,  Louisiana, California, and

-------
                    Texas - accounted for 88 percent of the total rice planted in the United States in 1993, and
                    89 percent of the total rice production for that year. The percentage of total rice treated
                    in Arkansas, Louisiana^ California, and Texas with thiobencarb was  14.1 percent.  The
                    average rate for thiobencarb used on rice was 2.85 Ib. Al/acre.
                             Ill             Ml                     |l'   I   I  I I I     .   .'"       .     •  :•  \   ' .;
                    D.     Regulatory History
                             II  i            11              i     I      li1'	:''   : •  .  •     .  .
                           TTiiobencarb  is  the  common  name  for  S-[(4-chlorophenyl)methyl]diethyl
                    carbamothioate.  Thiobencarb was first registered for use on rice in  1982.   In 1991,
                    .thiobencjrb'..was issued jregional tolerances for use on celery, endives, and lettuce in the
                    State of Florida.
                   ' ,i I,1 '.  '  .iHI!  .If,-!;1'1  v i!ป',;ซ	,	s, •!!",,/('!':•'. ii!!',',. ;•,!;'! * ''' ••;•:• c, . r, ', i" ..! ,.	ป  , j.'"... i-	i.         •   •           "

                           Currently,  there are five products containing thiobencarb registered under Section
                    3 of the Federal  Insecticide, Fungicide,  and Rodenticide Act.  They consist of one
                    technical  (manufacturing  use) product  containing 97.4 %  active ingredient,  two
                    emulsifiable concentrateend-use products  each containing 84.0%  active ingredient, and
                    two granular end-use products each containing 10.6% active ingredient.

                           Tie Agency issued a Data Call-In as part of the Phase 4 reregistration in August
                    1991. This Reregistration Eligibility Decision (RED) reflects a reassessment of all data
                    that were submitted voluntarily by the -registrant or in response to the Data Call-In Notice
                    attached to  this document.
             HI.    SCIENCE ASSESSMENT,... „!  ^."	"_  > ,', ..

                    A.     Physical Chemistry Assessment
l!!i

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                                                             N^/CHs
Empirical
Formula:
CAS Registry No.
                            A
                            \A
                                  257.S
                                  28249-77-6

-------
              Shaughnessy No.:    108401'
                                                                                   s.
                     Thiobencarb is a pale yellow liquid with a boiling point of 126-129ฐ  C.
              Thiobencarb is readily soluble in most organic solvents  and slightly soluble in
              water.-                          '"-.',
       B.     Human Health Assessment

              1.     Toxicology Assessment

                     At present, the available lexicological database for thiobencarb is adequate
              and will support reregistration eligibility for the currently registered uses.  Table
              1 lists the toxicity studies and their results for thiobencarb.

                     a.     Acute Toxicity

              Thiobencarb has been tested for acute toxicity by the oral, dermal and inhalation
       routes of exposure.   The results obtained in these studies, >which are listed in Table 2,
       satisfy the acute toxicity  data requirements.

Table 1. Acute Toxicity Values for Technical Thiobencarb.
Test
Oral LD50 - rat
Dermal LD50 - rabbit
Inhalation LC50 - rat ,
Eye Irritation - rabbit
Dermal Irritation - rabbit
Dermal Sensitization -
guinea pig
1 Results
Males: LD50 = 1033 (924-1155) mg/kg
Females: LD50 = 1130 (1033-1247) mg/kg (MRID 42130701)
LD50.> 2000 mg/kg (both sexes) (MRID 42130701)
LC50 > 42,8 mg/L (1 hour) (MRID 00040585, 00134976
Slight irritation (MRID 00040581)
Slight irritation (MRID 00040583, 00081900)
Not a sensitizer (MRID 00161699)
Toxicity ;
Category
in
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NA
r1
Purity
96.0%'
' 96.0%
95.1%
95.1%
95.1%
84.0%

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              b.     Subchronic Toxicity

       In a 21  day dermal study (MRID# 42893001, revision of MRID# 42003401),
 Sprague-Dawley rats received repeated dermal applications of Bolero* SEC (85.2% a.i.)
 at doses of 0, 40, 160, or 500 mg/kg, 5 days per week, over a 22-day period. Thirty-six
 animals of each sex were used, 6 animals/sex/dose for the 0, 40,  160 and 500 mg/kg dose
 plus an extra 6/sex/dose for,the 0 and 500 mg/kg doses at recovery.  There was a dose
 related increase in the incidence of skin irritation in treated versus control rats of both
 sexes. ]Jix^a
-------
levels in the high dose group. For systemic toxicity, the NOEL was 8 mg/kg/day and the
LOEL was 64 mg/kg/day based on increased liver and kidney weights, and decreased
hematological and  clinical chemistry parameters.   Based on biologically significant
depression in cholinesterase activity,  for  plasma cholinesterase,  the  NOEL was  1
mg/kg/day, and the LOEL was 8 mg/kg/day.  For erythrocyte cholinesterase, the NOEL
was 8 mg/kg/day and the LOEL was 64 mg/kg/day. For brain cholinesterase, the NOEL
was equal to or greater than 64 mg/kg/day and the LOEL was greater than 64 mg/kg/day.

              d.     Carcinogenicity

       In a carcinogenicity study (MKDD# 00086004),.B6C3F1 mice received 0, 25, 100,
400, or 1600 ppm (0, 3, 14,  56, and 235 mg/kg/day for males and 0, 5, 19, 75, 'and 302
mg/kg/day:for females, respectively) technical Bolero* (93.7% a.i.) for 121 weeks.
Systemic toxicity was noted at 14 mg/kg/day for males and 19 mg/kg/day for females and
higher as histopathological changes in the liver. These observations included an increased
incidence of  hepatocytic (glycogen) pallor; the high dose animals  also had increased
incidence, of fatty vacuolization (moderate or marked mid-zonar).  High dose males had
marked fine fatty periaciriar,  vacuolization, and increased relative heart and liver weights.
At 14 mg/kg/day and above, males had decreased absolute and relative kidney weights,;
while high dose females had increased relative kidney weights.  Upon gross examination,
there was an increased incidence of pale foci of the lungs in high dose animals, and pale
livers in the high dose males (external examination showed abdominal swelling). There
was also an increased incidence of focal epithelialization of the alveolar walls of the lungs
with associated macrpphages. In addition, high dose females had,reduced body weight
gains.  There was no evidence of carcinogenicity in either sex  at the dose levels tested.
For chronic toxicity, the NOEL was 3 mg/kg/day for males and 5 mg/kg/day for females
and the LOEL was 14  mg/kg/day for males and 19 mg/kg/day for females based on
histopathological changes in the liver.
             e.
Developmental Toxicity
       In a developmental toxicity study (MRID# 00115248), albino rats of the Sim: (SD)
FBR (Sprague Dawley derived) strain received by oral gavage either 0, 5, 25, or 150
mg/kg/day thiobencarb technical (97% a.i.) in Deionized Water/CMC/Tween 80 on days
6 through 19 of gestation.  Maternal toxicity was observed as a treatment related decrease
in body weight gains in the high dose group. There was no effect on food consumption;
however, the high dose had lower food efficiency than the control group, an indicator of
systemic toxicity. For maternal toxicity, the NOEL was 25 mg/kg/day and the LOEL was
150 mg/kg/day based on decreased body weight gains and decreased food efficiency.
Developmental toxicity was noted as a slight increase in  skeletal anomaly observations at
the high dose mostly related to reduced ossification and an increase in runts in the high
dose group. For developmental toxicity, the NOEL was 25 mg/kg/day and the LOEL was
150 mg/kg/day based on increased skeletal anomaly observations and an increase in the
number of runts.                          -               :

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       In another developmental toxicity study (MRID# 00164313), New Zealand white
 rabbits received 0, 20, 100, or 200 mg/kg/day technical thiobencarb (96.0% a.i,) by oral
 gavage from days 6 through 18 of gestation.  Maternal toxicity was observed at 200
jpl/kg/day as statistically significant increase in absolute and relative liver weights. For
 maternal toxicity,  the jsfOEL was 100 mg/kg7day and the LOEL was 200 mg/kg/day based
 on increased liver weights. No developniental toxiciiy was observed at dose levels tested.
 For developmental toxicity, the NOEL was equal to or greater than 200 mg/kg/day and
 the LOEL was greater than 200 mg/kg/day.

       Based on the  results of  these studies, thiobencarb is not considered to be a
 developmental toxicant in rats or rabbits.

             f.     Reproductive Toxicity

       In a multigenefation reproduction study (MRID# 40446201),  Charles River CD
 rats received1 either 0, 2, 20, or 100 mg/kg/day Te^            (96.7% a.i.) by daily
 oral gavage in 6.5% CMC aqueous solution.  Systemic toxicity was noted at 20 mg/kg/day
 and higher based on enlargement of cenrroloBuiaf hepatocytes (both generations), and
 hepatocyte smgle cell necrosis observed in both sexes of both generations including renal
 atrophic tubule consisting of regenerated epithelium. There were increased liver weights
 (absolute and relative) and increased kidney weights (absolute and relative) in the high
 dose group. There were also significant changes in body weights at 100 mg/kg/day and
 male kidney weights were increased in the high dose group.  There were no effects  on
 reproductive parameters.  For parental/systemic toxicity,  the NOEL was 2 mg/kg/day and
 the LOEL was 20 mg/kg/day based on histopathological changes of the liver and kidney.
 For reproductive toxicityj" 'the'NOEL was equal to or; greajer  than 100 mg/kg/day and the
 LOEL was greater ffian 100 mg/kg/day.

             g.     Mutagenicity

       Thipbencarb was evaluated in an Ames assay (MRID#s 00041174, 00084131 and
 00135285), and was negative in tester strains TAlOO, TA98 and TA1537 at levels up to
 50/ig/plate, both with and without metabolic activation.

       Thiobencarb was  negative in a dominant lethal assay in mice (MRID# 00084133
 and 00135282), administered at a single oral dose of 600 mg/kg, and at an oral dose of
 300 mg/kg for 5 days.

       In a clastogenicity test using human lymphocytes (MRID# 40352401), thiobencarb
 (96.0% a.i.) was tested at dose levels of 0, 5, 10, and 20 y^g/ml without S9 activation and
 at dose levels of 0, 10, 20, and 40fj,g/mL with S9 activation.. No mutagenic activity was
 noted.

       In a micronucleus test in mice (MRID# 40352402), thiobencarb (96.0% a.i.) was
 tested-at dose levels of 0, 270, 540, and 1080 mg/kg in males and at dose levels of 0, 405,

-------
 810, and 1620 mg/kg in females,  as a single oral dose.  A dose related increase in
 micronuclei was noted, and was statistically significant in high dose males and in the two
 highest doses in females.  Four consecutive daily doses of 540 mg/kg caused statistically
 significant increases in the incidence of micronuclei in both sexes.  This was considered
 as a positive mutagenic response.               '

       Thus, thiobencarb was "shown to lack mutageniciry in three of the four mutagenicity
 tests conducted.  No further testing is required at this time.

              h.     Metabolism

       In a general metabolism study (MRID# 42340302), the disposition and metabolism
 of [Phenyl-U-14C]-thiobencarb was investigated in male and female Sprague-Dawley rats
 at a single low oral dose (30 mg/kg), repeated low oral doses (30 mg/kg x 14 days), and
 a  single high dose  (300  mg/kg).   Thiobencarb  was rapidly  absorbed  after  oral
 administration as judged by the rate of excretion.  No significant sex-related or dose group
 differences in absorption were noted.  Excretion was relatively rapid at all doses tested,
 with a majority of radioactivity eliminated in the urine and feces by 48 hours.. The extent
 of excretion was  completed by 72  hours at  the 300 mg/kg  dose,  but the mechanism
 responsible for this  delay was  not identified.  No significant sex- or  dose-related
 differences in urinary or fecal excretion of thiobencarb derived radioactivity were noted.
 Repeated low oral dosing did not affect elimination of thiobencarb in either male or female
 rats.                           '.     ,                                   ,

       Fecal elimination of [Phenyl-U-14C]-thiobencarb derived radioactivity was a minor
 route of excretion, and for urine, no significant sex- or dose-related differences in amount
 of radioactivity excreted  by this route were observed.  Residual levels of thiobencarb
 derived radioactivity were also minor (less than 0.5% of an administered  dose).

       Urinary'and fecal metabolites of [Phenyl-U-14C]-Thiobencarb were  isolated and
identified by HPLC, TLC, and mass spectral analysis.  The major metabolite detected was
the  glycine  conjugate 4-chlorohippuric acid,  comprising  between  74-81%  of an
administered dose in urine.  Other metabolites detected included 4-chlorobenzyl methyl
sulfoxide and -sulfone, des-ethyl thiobencarb, and 4-chlorobenzoic acid, each representing
less than 10% of an administered dose of thiobencarb.  A single high or repeated low oral
dose did not significantly affect the urinary or fecal metabolite profile for thiobencarb in
male or female rats.                        ,

              i.     Neurotoxicity

       Acute Neurotoxicity

       In an acute neurotoxicity study (MRID# 42987001, 43148202),. male and female
Sprague-Dawley rats received a single oral administration of thiobencarb (96.9%) at doses
                                     11

-------
M	; i; ], i"" ,'i1
                              "ili: • i,,:: fi,,,:	!" > Hit1'ป;:ซ	c .j i' •.	:.;.. it i'i=- • MiV liia'	i-' "i' li-i'tS-	,.(, Jijji;: ,;
                     < -, !   WE! i "lii, in.: . ft	  '.iaSflK!!!!	i'-ff:	.•:.•ป i i'."MV'ij*! Tii	>	 K"i	*i	(V'.'Si >,i •.i'.fWHiHT*  •>  '. ', .•  •'., '     '	;..,!'
                    of 0,  1Q0, 500 or 1 GOO mg/kg.  Neurqbehavioral evaluations, consisting of Functional
                    Observational Battery (FOB) and motor activity, were conducted at pre-study, day 0, at
                    time of peak effect  (4 firs post-dosing), day 7 and day 14.  At day 15, animals were
                    euthanized and neuropathological examination performed on control and high-dose animals
                    (5/dose/sex).  With the exception of one high-dose female, which died on day 3 of the
                    study, all other animals survived until terminal sacrifice.  An increased incidence of
                    clinical signs, consisting  of red deposits around the noses  and mouths  of high-dose
                    animals, were noted.  Gait abnormalities (rocking, lurching and swaying) were observed
                    in some High-dose; females. No significant differences were noted in either the mean body
                    weight or body weight gain of any of the treated animals.  Neurobehavioral evaluation
                    revealed treatment-related FOB and motor activity findings at the mid- and high-dose
                    levels. The effects were, in general, transient and observed only at the peak time of effect
                    (4 hrs gost-dosing).  Althpugh  the incidences of FOB  findings were not significantly
                    different from control values, when taken together, a consistent, treatment-related pattern
                    of neurobehavioral effects becomes clear.  These findings included gait abnormalities
                    (lurching, swaying and rocking), impaired mobility,  and decreased sensory responses
                    (approach", touch, startle, tail pinch aiio! pupil responses). In high-dose males, the startle
                    response achieved statistical significance when  measured at the time of peak effect.
                    Hindlimb resistance was reduced in high-dose animals"  Mean  body temperature'was
                    significantly decreased in all treated males and mid- and high dose females. Total and
                    ambulatory  motor activity, measured at the peak time of  effect  on day  0, showed
                    significant treatment-related decreases in all mid- and high-dose animals. No treatment-
                    related gross or neuropathological findings were present. Brain weights and measurements
                    of the treated animals were comparable to control values. Thus, for systemic toxicity, the
                    NOEL was 100 mg/kg/day and  the LOEL was 500 mg/kg based on increased clinical signs
                    and gait abnormalities. For neurobehavioral toxicity, the NOEL was  100 mg/kg/day and
                    the LOEL was 500  mg/kg based on gait abnormalities, decreased sensory responses,
                    decreased body temperature, and decreased motor activity.

                    Subchronic neurotoxicity
                           li| a s,u|?chronic neurotoxicity study (MRID# 43001001), male and female Sprague-
                    Dawley rats (lO/sex/groupJ received oral administration of  Bolero* SEC (89 percent
                    purity) at 0, 2, 20 or 100 mg/kg/day for 13 weeks.  All animals survived until terminal
                    sacrifice.  Clinical signs were evident only within the first 4-hours post-dosing.  During
                    this time, there was an increased incidence of dried red material around the noses of all
                    treated animals and dried tan or red material around the mouths of mid- and high-dose
                    gnirnals.  Mean body weights and body weight gains of high-dose females were lower than
                    controls.  Food consumption was not affected by treatment.  The absolute-and relative (to
                    terminal body weight and brain weight) liver and kidney weights" of high-dose males and
                    females was statistically significantly increased. The relative (to the terminal body weight)
                    liver weights  of mid-dose males  and the kidney weights of mid-dose  females were
                    statistically significantly increased.  No  clinical pathology was conducted. In addition, no
                    treatment-related gross or neuropathological findings were present.  Thus, for systemic

                        •'  '-	 '	   '  	:' "•"• "•  ' "  ::::	12-   	"'	,

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toxicity, the NOEL was 2 mg/kg/day and the LOEL was 20 mg/kg/day based on increased
clinical signs, decreased body weights,  increased  liver and  kidney weights.  For
neurotoxicity, the NOEL was greater than 100 mg/kg/day (HDT) and a LOEL was not
established.

              j.     Endocrine Disrupter Effects

       EPA is required to develop a screening program to determine whether certain
substances (including all pesticides and inerts) "may have an effect in humans that is
similar to an effect produced by a naturally occurring estrogen, or such other endocrine
effect.:." The Agency is currently working with  interested stakeholders, including other
government agencies, public interest groups, industry and research scientists in developing
a screening and testing program and a priority setting scheme to implement this program.
Congress has allowed 3 years from the passage of FQPA (August 3,  1999)  to implement
this program. At that time, EPA may require further testing of this active ingredient and
end use products for endocrine disrupter effects.

              k.     Epidemiological Information

       No cases of poisoning were located from any of the available databases on incidents
related to the use of thiobencarb.  EPA believes that this may be due  partly to the
relatively limited use of this chemical.               :

       2.      Toxicological Endpoints for Risk Assessment

              a.     Reference Dose (RfD)

       The RfD/Peer Review Committee met on February 8,  1996, to discuss and
evaluate  the existing and/or recently  submitted toxicology  data  in support  of the
thiobencarb reregistration and to reassess the RfD for this chemical.

      , The  Committee  recommended  that the existing  RfD for thiobencarb, remain
unchanged.  The RfD for this  chemical was based on the two year rat feeding study
(MRID# 00154506) with a NOEL of 20 ppm (1 mg/kg/day).  At the next higher dose
level of 100 ppm (5mg/kg/day), decreased body weights and increased blood urea nitrogen
levels were observed. An uncertainty factor of 100 was applied to account for both inter-
species extrapolation and intra-species variability.  On this  basis, the RfD was calculated
by the Committee to, be 0.01 mg/kg/day.

             b.     Carcinogenic Classification

       The carcinogenic potential of thiobencarb was evaluated by the RfD/Peer Review
Committee on February 8, 1996. The Committee considered the carcinogenicity phases
of the combined chronic toxicity/carcinogenicity studies in rats (MRID# 00154506) and
the carcinogenicity study in mice (MRID# 00086004)  for carcinogenic classification.

         '             '             13             •''.".''

-------
   "i; i . . " • Wl! I'll1*:: t ',; !" ..... ........ " ! • Si' ....... 'if it: •!
                       f f ซf :ซ*: V;.;1'!;;!! I1' * ;'T ;" ..... 'ป(!. ' 111- ...... ; 1, Si ..... SI ;!"> 1 ..... 1 ...... 1 "Will 7T
       The highest dose level tested in rats (500 ppm, or 25 mg/kg/day) was considered
to be adequate for carcinogenicity testing based on depression of cholinesterase activity
and reduced body weight gain.  The highest dose level tested in mice (1600 ppm, or 235
mg/kg/day in males and 302 mg/kg/day in females) was considered to be adequate based
on body weight gain depression.

       In rats, there was nq treatment-related increase in tumors of any kind at any dose
level.   The Committee,  therefore,  concluded that the treatment did not  alter the
spontaneous tumor profile in this strain of rat.
       In mice, adenomas and carcinomas of the harderian glands appeared to be increased
in treated females (1,. 2, ^6tlS'^Jf^p^foijh&Q~ 25,' 10, 400 and 1600 ppm groups,
respectively).  However, the Committee noted several limitations with the study. First,
the concurrent control incidence was lower than expected for females of this strain. This
decreased incidence in the control group could possibly be due to chance and could not be
precluded. Second, if a greater number of control mice had lived until completion of the
study, more spontaneous tumors may have occurred, thus resulting in similar tumor
incidence between txeatoent and control groups. Third, the  study was carried out for 121
weeks, a significantly  longer period than guideline requirements.  Thus,  the increased
study length may have contributed to the appearance of tumors in treated females.  Fourth,
the Committee concluded that historical control incidence data from studies conducted for
a significantly shorter duration should not be considered.  The Committee reasoned that
these shorter  duration studies may not  accurately depict tumor  incidences because the
tumor incidence would most likely be lower than what was observed in the studies used
for carcinogenic classification. Thus, no historical control data were acceptable for review
by the Committee.
       On this basis, the Committee recommended that thiobencarb be classified as Group
P chemical (not classifiable as to human carcinogenicity).
c.
                           lexicological Endpoints
       IH!!
       On April 30, 1996, the Agency's Office of Pesticide Program Health Effects
Division Toxicity Endpoint Selection Committee (i.e. the TES Committee), met to discuss
the toxicological endpoints to be used in various risk assessments for thiobencarb.  A
summary of the endpoints selected is provided in Table 3.

       Dermal Absorption.  In addition to the toxicological endpoints listed in Table 2,
the TES Committee discussed the dermal absorption of thiobencarb.
                                    14

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Table 3. Summary of Toxicological Endpoints for Thiobencarb
Type of Exposure
Acute Dietary (one/day)
Short-Term Occupational
or Residential Exposure
(one to seven days)
Intermediate-Term
Occupational or
Residential Exposure, (one
week to several months)
NOEL
NOEL = 25 mg/kg/day established in a rat developmental
toxicity study (MRID 00086873, 00093691 and'
00115248)
NOEL = 25 mg/kg/day established in a rat .developmental
toxicity study (MRID 00086873, 00093691 and
00115248). .
NOEL = 2 mg/kg/day for systemic toxicity established in
a rat subchronic neurotoxicity study (MRID 43001001). '
This NOEL of 2 mg/kg/day is supported by a similar
NOEL (2 mg/kg/day) established in the multigeneratidn
reproduction study (MRID 40446201 and 409085701).
Endpoint
Increases in incidence of
reduced ossification and an
.increase in fetal runts.
Increases in the incidence
of reduced ossification and
an increase in fetal runts.
Histopathological changes
in the liver and kidney.
             In a dermal absorption study (MRID# 41215311),  Sprague-Dawley* CrliClf
       (SD)BR male rats were dermally treated with either 0, 0.05, 0.5 or 5.0 ing/rat of 14C-
       Thiobencarb (Radiochemical purity: 98.8%,  Specific Activity: 359,092 dpm/^g)  for
       exposure durations of 1, 2,  4,  10, or 24 hours (4 rats per dose per duration).  The
       unlabeled compound used was Bolero* SEC (Thiobencarb, Purity: 89% a.i.). This study
       may represent a worst-case scenario since the skin was washed approximately 1 hour prior
       to dosing rather than the recommended 24 hours (which would allow normal replacement
       of skin.oils).  Thus,  this might tend to over-estimate absorption. Based on the results of
       the study, the Committee determined that thiobencarb is rapidly and continuously absorbed
       at doses of 5.0, 46.8 and 498 Aig/cm2 for exposure times up to 24 hours. Absorption at
       10 hours was 60.2, 52.6, and 17.1% for the 5.0,  46.8 and 498 ^g/cm2 dose  groups,
       respectively.  Maximum absorption at 24 hours was 71.5, 72.6, and 41.75 for the 5.0,
       46.8 and 498 yUg/cm2dose groups, respectively.  Urine was the primary route of excretion.

             On this basis,  the committee recommended that a dermal absorption factor of
       60.2%, observed at 10 hours in a dermal absorption study (MRID# 41215311),  be used
       for risk assessment purposes, even  though the Committee recognizes that this value is
       likely to over-estimate absorption.   A new dermal absorption study is requested  for
       confirmatory purposes.                            '

       Summary of Science Findings                           ,,

       i. Directions for Use             '    ...                         •

             A tabular summary of the residue chemistry science assessments for reregistration
       of thiobencarb is presented  in Appendix  3.   The  conclusions listed  in  Appendix 3
       regarding the reregistration eligibility of  thiobencarb  are based on the use patterns
       registered by the basic producer.  All end-use product labels (e.g., MAI labels, SLNs, and
       products subject to  the  generic data exemption)  must be amended such that they  are
       consistent with the.basic producer labels.
                                          15

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Table 4.  Chemical structures of thiobencarb and its metabolites containing the chlorobenzyl and
chlorophenyl moiety   	_____
  Compound: thiobencarb
Compound: Thiobencarb
                          /CH3

                         I	 'i, ,!!"i||,!:i	B'UlVt'll "." "' (I'l,,!!1 :'i	" .' 1,'liW" '.,f!l 'f'' '
  Thiobencarb
  S-((4-Chlorophenyl)methyl)diethylcarbamothioate
            4-Chlorobenzy]methylsulforie
                          OH
              4-Chlorobenzoic acid
                                                                                OH
                                                             N-(4-Chlorobenzoyl)glycine
                        Conjugate
          4-Chloroben2ylthio conjugates

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Table 5.  Thiobencarb end-use products (EPs) with food/feed uses.
Registrant
Valent U.S.A.
Corporation
K-I Chemical
U.S. A Inc.
,EPA Reg, No.
59639-79 /
59639-80 2
63588-5
63588-6
Acceptance Date
4/20/94
11/29/93
2/94
2/94
Formulation
8 Ib/gal EC
10.% G
10% G
81b/galEC
Product Name
Boleroฎ 8EC (Herbicide)
Boleroฎ 10 G (Herbicide)
Boleroฎ 10 G
Boleroฎ SEC •
  EPA Reg. No 59639-79 is the parent label for the following Section 24ฎ registrations:  AR940002, AR940003,
AR950004, CA930003, FL910003, FL930010, LA950005, MO930007, MO940005, MO950002, MS930009,
MS930010, MS950007, TX930023, andLA960004.                        .    ' '                  '
2 EPA Reg. No. 59639-80 is the parent label for flie following Section 24ฎ registrations: AR940001, MS930011 and
TX930024.

ii. 'Plant Metabolism
                                             • ,  i            '         ',    -
       The  qualitative nature of the, residue in plants is adequately understood based on an
acceptable study depicting the metabolism of thiobencarb in rice.  On  May  13, 1993, the
Agency's Metabolism Committee determined that the current tolerance expression for residues
of thiobencarb and its metabolites containing the chlorobenzyl  and chlorophenyl moieties  is
appropriate.

iii.  Animal Metabolism

       For the purposes of reregistration and risk assessment, the qualitative nature  of the residue
in animals is adequately understood based on acceptable studies conducted on ruminants and in
poultry. The residue of concern in eggs, milk, and poultry and livestock tissues include the parent
thiobencarb and its metabolites containing the chlorobenzyl and chlorophenyl moieties.  The
current tolerance expression for animal commodities,  as defined in 40 CFR ง180.401(a),  is
adequate.   ,               .          '    •    ;    •

iv.  Residue Analytical Methods - Plants and Animals

       The  requirements for_ residue analytical methods are fulfilled  for the purposes of
reregistration.  Adequate methods are available for enforcement and data collection purposes for
both plant and animal commodities.  Successful radiovalidation of the enforcement methods, using
samples from the metabolism studies, has also, been conducted.  The 1994 FDA PESTDATA
database indicates that residues  of thiobencarb are  completely  recovered (>80%) using
multiresidue  method Section 302  (Luke method; Protocol D), and variably recovered using
method Section 304  (Mills, Onley, Gaither method; fatty food).  The registrant has conducted
multiresidue  method trials  with  thiobencarb  metabolites . 4-chlorobenzylmethylsulfone  and
4-chlorobenzylmethylsulfoxide using Protocol E and with 4-chlorobenzoic acid using Protocol B.
The Agency has forwarded the results of these multiresidue trials to FDA for evaluation and
inclusion in  Pesticide Analytical Method (PAM) Vol. I, Appendix I.                      •-
                                           17

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v. Storage Stability

       Adequate storage stability data are available to support the established tolerances.
Acceptable storage stability studies have been submitted for representative plant and
animal commodities. The available plant and animal metabolism studies are also validated
by adequate storage stability data.

vi. Magnitude of the Residue in Plants

       The  reregistration requirements for magnitude of the residue in/on plants are
fulfilled for the following commodities: celery, endive, lettuce, rice grain and straw. No
additional data are required.  Adequate field trial data, following treatments according to
the maximum registered use patterns, have been submitted for the commodities listed
above.  The available data were submitted in  conjunction with tolerance petitions for
celery, endive, and lettuce (PP#5F3158), and rice grain and straw (PP#OF2322, 5G1582,
6F1763, and 2G1231), and are adequate to support reregistration requirements including
tolerance reassessment.

vii.  Magnitude of the Residue in Processed Food/Feed

       The  reregistration requirements for magnitude  of the residue in the processed
food/feed commodities of rice are fulfilled.  An acceptable study depicting the potential
for thiobencarb residues of concern to concentrate in rice processed fractions has been
submitted and evaluated. The data indicate that the combined residues of thiobencarb and
4-chlorobenzylmethylsulfone did not concentrate in polished rice and bran processed from
rice samples that received postemergence application of the registered 10%  G formulation
at an exaggerated rate  (5x). However,  the combined residues concentrated 2x in hulls.
Although residue concentration was observed in hulls, the observed combined residues of
thiobencarb  and  its metabolite (
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ix. Magnitude of the Residue in Potable Water                                   '

       The reregistration requirements for magnitude of the residue in 'water will be
considered fulfilled when revisions are made to Valent's end-use product labels (EPA Reg.
Nos.  59639-79 and 59639-80) to prohibit use of treated water for livestock watering or
for drinking or irrigation for a specified time period after treatment.  Based on the results
of an acceptable magnitude of residue  in, potable water study  (MRIDs 43404003,
43404004, and 43404005),  thiobencarb and thiobencarbsulfoxide residues in runoff and
receiving waters associated with rice fields did not fall to acceptable levels until i4 days
after  treatment.  Thus, the Agency has determined that a 14-day water holding interval
should be imposed following thiobencarb applications to rice fields.  If the registrant does
not wish to institute this label restriction,  then a irrigated crop field trial and a drinking
water treatment intake study will be required.                    •

       The use of the thiobencarb granular formulation (Boleroฎ 10G, EPA  Reg.  No.
59639-80) in California is regulated under the Basin Plan for the Sacramento River Basin
established by the California Regional Water Quality Control Board, Central Valley
Region.  A performance goal of 1.5 ppb is strictly monitored, and growers must adhere
to a  program of approved management  practices, including a 30-day water  holding
restriction.                                                ,  .

x. Nature and Magnitude of the Residue in Fish

       The reregistration requirements for nature and magnitude of the residue in fish will
be fulfilled when label revisions are made on Valent's end-use products (EPA Reg. Nos.
59639-79 and 59639-80) to specify the following,use restrictions:  "Do not use on rice
paddies where commercial catfish or crayfish farming is practiced.  Do not use adjacent
to catfish or crayfish ponds."
                                                        \
xi. Magnitude of the Residue in Irrigated Crops

       Data depicting the magnitude of the residue iri irrigated crops are not required for
reregistration purposes since the Agency is imposing a 14-day water holding interval.

xii. Magnitude of the Residue in Food-Handling Establishments                   •

       Thiobencarb is not registered for use in food-handling establishments; therefore,
no residue chemistry data are required under this guideline topic.

xiii.  Confined/Field Rotational Crops

       Valent's thiobencarb end-use labels specify a 6-month plantback interval following
rice and all other crops, except celery, endive and lettuce for which rotational crop plant-
                                     19

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 back intervals  are 4-months.   These  currently specified  plant-back  intervals  are
 appropriate.
>:'"'.,,; ' II • 'T ..... . , ' ' J; , ' . :  , S i • i; , ::i; , :: !? . .;'. | ' , ; Iji f : ' '* = j> ; ; > '} , , •' j .; if •>;< ' !;ป "i • ft >• I; -| ,y ;.;.'; j :',:„'  . .   •• ' • .  •     . •   "  '  ; ,
      Residue Information (Tor dietar  risk assessment)
       Tolerances for thiobencarb  are published in 40  CFR  180.401(a)  and (b).
 Tolerances have been established for rice grain at 0.2 ppm; meat, fat, and meat byproducts
 of cattle, goats, hogs, horses, poultry, and sheep at 0.2 ppm; eggs at 0.2 ppm; and milk
 at 0.5 ppm. Tolerances with regional registrations are established for celery, endive, and
 lettuce at 0.2 ppm each.   Tolerance  level  residues and  100 percent crop treated
 assumptions were made for all commodities.  Anticipated residue information was not
 used for this analysis.

   ;      d.   Dietary Exposure from Drinking Water

       Thiobencarb is not currently regulated under the Safe Drinking Water Act.  Public
 water supply systems are not required to sample and analyze for thiobencarb.  Thus, no
 Maximum Contaminant Level (MCL) is established for thiobencarb in drinking water
   j    I, nil |                 t   \     /               ,, „ ...... , ..... .I,,,,,, ,„,; ..... , ..... ,     ,,, ,             O
 systems^ An MCL is an action level as established by the EPA Office of Water to ensure
 the safety of drinking water.  In addition, a lifetime Health Advisory (HA) for thiobencarb
 has not been established.

 Ground Water
         Limited groundwater monitoring information is available for thiobencarb.  The
 "Pesticide in Ground Water Database" (Hoheisel et al., 1992) reported sampling for
 thiobencarb in 270 wells in California and 65 wells in Missouri.  Two detections of
 thiobencarb in ground water were reported in Missouri and at very low concentrations (0.2
 - 0.3 ppb).  However, no limit of detection (LOD) or limit of quantification (LOQ) was
 provided to normalize  the data for non-detectable residues.  This  is  an  important
 consideration since thiobencarb was not detected in almost all wells  sampled. Therefore,
 the groundwater sampling data are not usable for drinking water risk  assessment purposes.

 Surface Water

        Thiobencarb has the potential to contaminate surface water from releases of rice
 paddy water following thiobencarb applications or from spray drift associated with aerial
 or ground spray application to other registered sites.

        The Agency  provided estimates of thiobencarb residues  in surface water by
 utilizing the Generic Estimated Environmental  Concentration program (GENEEC),
 EPA's  Office of Water's STORET database and data  from a California thiobencarb
 surface water monitoring study.  However, the GENEEC and STORET data were not
 applicable to access thiobencarb in drinking water.  First,  GENEEC  does not  model for
                                    20

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rice scenarios.  Since almost 95% of.all thiobencarb use is on rice, GENEEC exposure
estimates are not applicable to most thiobencarb uses.  Second, the STORET data were
not normalized for non-detectable residues.  Approximately 99% of samples collected to
measure for thiobencarb indicated non-detectable residues.  Therefore, due to limitations
with the GENEEC and STORET data, the California surface water modeling study was
the only applicable data to measure thiobencarb in surface water.  The results of the
California thiobencarb surface water monitoring data are provided below.

California Surface Water Monitoring study

       Monitoring for residues of specific rice pesticides in surface water of California's
Sacramento River basin was  performed by the California  Environmental Protection.
Agency (CAL EPA), sometimes in  conjunction  with the  California Rice  Industry
Association, from 1993 to 1996. The Agency estimates that  the City of Sacramento is the
only locality in the US rice growing region relying on surface water as its source of
drinking water (i.e. the city utilizes the Sacramento River as its  source of drinking water).

       In 1993, 17 samples  were collected just before the intake to the Sacramento River
drinking water treatment facility (the only year of the four year study that samples from
this location were collected). No detections above a limit  of detection of 0. l//g/L were
reported. However in 1993, due to substantial flow in the Sacramento River, water was
diverted south of the sampling location via the Yolo Bypass'. Thus, diverting water from
the Sacramento River  drinking water  treatment facility  may have  contributed  to
thiobencarb levels below the limit of detection;  Therefore, The Agency concludes that
even though thiobencarb residues at  the Sacramento  River were below the limit  of
detection (0.1 (J,g/L), thiobencarb residues may be higher if water was not diverted via the
Yolo Bypass.

         e.    Occupational Exposure

       An occupational and/or residential exposure assessment is required for an active
ingredient  if (1) certain lexicological criteria are triggered  and .(2) there is potential
exposure to handlers (mixers, loaders,  applicators, etc.) during use of to persons entering
treated sites after application is complete.

       In  the case of thiobencarb, EPA has determined  that there is  a toxicological
concern and there is potential exposure to mixers, loaders, applicators, or other handlers
during activities that would occur under the usual thiobencarb use scenarios.  Also, there.
is potential exposure to persons reentering sites that have been treated with thiobencarb.
Therefore, the  Agency has  assessed application and post-application -exposure  to
thiobencarb.

       At  this  time,  products containing thiobencarb  are intended primarily  for
occupational uses only and not for homeowner uses. Thus, this exposure assessment is
                                    21

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limited to occupational uses only. Further, EPA expects that, based on the use patterns,
exposure to thiobencarb will occur for a short to intermediate duration; chronic exposure
is not expected.  Finally, the Agency expects exposure to  occur via the dermal and
inhalation routes.

i.  Handler Exposure
       EPA has identified eight major exposure scenarios from  the use patterns of
thiobencarb for its occupational exposure assessment: (la)  mixing/loading liquids for
aerial application; (Ib) mixing/loading liquids for groundboom application; (2a) loading
granulars for fixed-wing aircraft;  (2b)  loading  granulars  for tractor-drawn spreader
application; (3) applying sprays with a fixed-wing aircraft; (4) applying granulars with a
fixed-wing aircraft; (5) applying sprays with a helicopter; (6) applying granulars with a
tractor-drawn  spreader;  (7) applying sprays with groundboom equipment;  and, (8)
flagging aerial spray applications.
       Potential dermal and inhalation baseline unit exposure (which are derived from
PHED V, 1.1), along with corresponding calculated daily exposures, are presented in
Table 6.  No chemical-specific data were submitted. Baseline unit exposure is the PHED
exposure estimate with baseline attire (long-sleeve shirts., long pants, shoes, and socks).
Dermal exposure is several orders of magnitude greater than inhalation exposure.

Potential daily exposure is calculated using, the following formula:

Daily exposure (mg ai /day ) =  Unit exp. (mg ai/lb ai) x Max. App. Rate (Ib ai/ac) x Max. Area Trt.
(ac/day)
                                    22

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 ii. Post-Application Exposure

        Based  on the use patterns of thiobencarb, EPA has determined that  there is
 potential exposure for persons entering treated sites after application is complete. Workers
 may be entering treated areas to perform such tasks as scouting, thinning, or hoeing.
 However, there are no chemical-specific data available upon which to assess the risks from
 post-application  exposures.  Since no dislodgeable foliar residue studies or concurrent
 dermal samples  were submitted to the  EPA for this  RED to measure post-application
 reentry, a rough surrogate post-application assessment was performed as a default.  The
 surrogate assessment is  based  on the  range of application rates  (4-6  pounds active
 ingredient per acre), the assumption that 20%  of the initial application  is available as
 dislodgeable residue after sprays have dried or granules have settled (approximately 12
 hours after application is complete), and a residue dissipation rate of approximately 10%
 per day. The short-term endpoint (25 mg/kg/day) was used for the risk assessment, since
 EPA does  not anticipate that intermediate-term exposures  (i.e., 7  days or more of
 exposure) are  likely to occur for post-application workers.   EPA also assumed in this
 surrogate assessment that dermal absorption would be significantly lower that the 60.2
 percent used in the handler assessment, since dermal exposure would be to dry residues.
 Thiobencarb is applied early season, therefore late season tasks, such as harvesting, are
 not a concern.  The tasks of concerns, including scouting, thinning,  and hoeing, are not
 likely to be of long duration.   Therefore the Agency chose a transfer coefficient that
 reflects the  relatively low dermal transfer.

               f.     Aggregate Exposure

       In examining aggregate exposure,  FQPA requires that EPA  take into  account
 available information concerning exposure from the pesticide residue in food and all other
 exposure for which there is reliable information.  These other  sources of exposure of the
 general  population  (including  infants and  children)  to pesticides include residues in
 drinking water  and non-occupational exposures, e.g.  to pesticides used in and around the
 home and to  sources not directly related to use of the pesticide  such as Superfund sites or
 TRI emiงs.iqns. Only food source and drinking water exposure Were evaluated since no
 non-occupational use is expected.

     •'• 3.	  Risk Assessment 	            '

       EPA expects both dietary and occupational exposure from the use of thiobencarb
 (there are no  residential uses).  Dietary  exposure  occurs  via the  oral route while
 occupational exposure occurs via the dermal and  inhalation routes.  Since an acute
 inhalation study placed thiofaencarb in Toxicity Category IV, an inhalation risk assessment
	w,as npt initiated,   	, ,	,	          ,        .  ,    ,
                                    24

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       Dietary exposure (food and drinking water sources) is expected to occur over an
 acute through chronic period.  To assess the acute dietary risk, EPA calculates a margin
 of exposure (MOE), which is the ratio of the NOEL to exposure.  To assess chronic risk,
 EPA calculated the percent of-the reference dose [RfD] (i.e. % RfD) occupied.

              a.     Dietary Risk

 L- Acute Dietary (Food Source) Risk

       The Dietary  Risk  Evaluation System (DRES)  acute  analysis  estimates  the
 distribution of single-day exposure for the overall U.S. population and certain subgroups.
 It includes all published uses of thiobencarb, even those commodities that  are  being
 recommended for revocation.  The analysis evaluates individual food consumption as
 reported by respondents in the USDA 1977-1978 Nationwide Food Consumption Survey
 and accumulates exposure to the chemical for each commodity.

       The MOE is calculated by dividing the acute dietary NOEL (i.e. mg/kg/day) by
 the high-end exposure (see Table 2 of Appendix 5 for the exposure estimates).  High-end
 exposure represents  exposure of the pesticide to 99.5% of the targeted population.
 Because the  endpoint of concern'for-acute  dietary, risk assessment is a developmental
 toxicity effect, the only subgroup of concern is females (13+  years).  Generally,  acute
 aggregated MOEs greater than 100 tend to cause no dietary concern when the data are
 compared  to a  lexicological  endpoint from  an animal  study  (such  is the case for
 thiobencarb).  Since the  only subgroup of concern is females  (13+) and represents an
 MOE = 8928,the Agency is not concerned with acute dietary risks from exposure to
 thiobencarb residues in food,

 ii.  Acute Dietary (Prinking Water) Risk

       Due to limitations with available groundwater sampling data, no groundwater data
 are applicable for risk assessment purposes.  Even though there is an absence of applicable
 groundwater data, based on the environmental fate of thiobencarb and the soil profile of
 rice fields, the Agency does  not believe  that thiobencarb  would be  a concern to
 groundwater.  First, thiobencarb is slightly persistent in water, generally not very mobile,
 tends to bind to soil organic matter, and doesn't desorb. .Second, rice fields are usually
 underlain by a clay layer to restrict water movement through the soil and help contain the
 water in the flooded field. This clay layer will-significantly limit the amount of leaching
 that occurs in rice fields.;                     .    '

      As noted above, approximately 9.5% of thiobencarb applications are made to rice.
In addition, the Agency estimates that the City of Sacramento is the only locality in the US
rice growing region utilizing surface water as its drinking water. Data from the California
 surface water monitoring study indicated thiobencarb residues were not above  a limit of
                                    25

-------
detection of 0.1 /zg/L (0.1 ppb).  Thus, drinking water exposure was calculated using the
following formula:

Exposure (mg/kg/day) =  (ppb thiobencarb in the water consumed) (10 6)(33.3)

       Thus, drinking water exposure = 3.33 x 10'6 mg/kg/day and  a resulting MOE >
10,000.  Therefore, the Agency is not concerned with acute drinking water risks from
exposure to thiobencarb residues in drinking water.

       Water consumption is defined as all water obtained from the household tap that is
consumed either directly as a beverage or is used to prepare foods (mixing water with a
can of soup) and beverages (diluting frozen juice concentrate). Since a developmental
endpoint was selected for acute dietary exposure, the subpopulation of  concern are females
(13+ years).   This subpopulation are assumed to weigh 60 kg "and consume 2.0  liters of
water per day (33.3 g/kg-body wt/day), the value used in the above equation.

iii. Total Acute Dietary CFood Sources and Drinking Water)
                                                                   y*

To assess total acute dietary exposure and MOEs, the following formulas were utilized:
Total acute dietary exposure  =     acute food source exp. (high end exp.) [mg/kg/day]
                                "': + drinking water exp. (high end exp.) [mg/kg/day]

Total acute dietary MOE=  NOEL ('mg/kg/day')
                                  total acute^ dietary exposure .(mg/kg/day)

       Food source exposure (high end exposure) was 0.0028 mg/kg/day based on the
high end exposure for females (13+ years).  Drinking water exposure (high end exposure)
was 3.33 x 10"6 mg/kg/day based on surface water exposure as discussed previously.
Thus, the total acute dietary MOE is 8920. Therefore, The Agency is not concerned with
total acute dietary risks from exposure to thiobencarb.

iii.  Chronic Dietary (Food Source*)  Risk
       A DRES chronic exposure analysis was performed using tolerance level residues
and a 100 percent crop treated assumption to estimate the Theoretical Maximum Residue
Qon|ribjitio|i (TMRC) Jpr the general population and 22 subgroups.  A summary of the
TMRCs and me % RfD values for the U.S. general population, non-nursing infants (< 1
year  old) children  (1-6 years) and females (13+ years) are provided in Table 2  of
Appendix 5. The chronic analysis for thiobencarb is a 'worst case estimate of dietary
exposure with all residues at tolerance level and 100 percent of the commodities assumed
to be treated with thiobencarb.
                                    26

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             As shown in Table 7, much less than 100% of the RfD is occupied by the dietary
       uses recommended through reregistration.  Existing tolerances result in a TMRC which
       represents 12.8% of the RfD for the U.S. general population. The highest subgroup,
       Non-Nursing Infants (< 1 year old), occupies 42.9% of the RfD.  In addition, numerous
       conservative assumptions have been considered into this assessment.  Thus, the actual %
       RfD is considered .<. 42.9%. Therefore, The Agency is not concerned with chronic
       dietary risks from exposure to thiobencarb residues in food.

Table 7. Chronic Dietary (Food Source) Risk Evaluation (based on TMRC).
- Population Subgroup
- General U.S. Population
Non-nursing infants (< 1 year)
Children (1-6 years)
Females (13 + years)
Exposure (mg/kg/day}
0.001280
0.004294
0.002945
0.001103
%RfD (Chronic - noncancer)
12,8
42.9
29.5
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       iv.  Chronic Drinking Water Risk                    .     '   •.      -

             As noted previously, due to limitations with available groundwatef sampling data,
       no groundwater data are applicable for risk assessment purposes. However, even though
       there is an absence of applicable groundwater data, based on the environmental fate of
       thiobencarb  and the soil profile of lice fields.   The Agency does not believe that
       thiobencarb would be a concern to groundwater.

             The Agency utilized data from the California surface water monitoring study to
     -  access chronic drinking water exposure.  A drinking water exposure estimate of 0.1 //g/L
       (i.e. 2.86 x 10"6 mg/kg/day) was used to assess chronic exposure (as was performed for
       acute  drinking water  exposure) since this  is the .only data available.  As was noted
       previously in the acute drinking water exposure assessment, data from the California
       surface water monitoring study indicated thiobencarb residues were not above the a limit
       of detection of O.l^g/L.  Thus, high end drinking water exposure was utilized for the
       chronic drinking water risk assessment and corresponds to a %RfD = 0.29.  Therefore,
       The Agency is not concerned  with  chronic  drinking  water  risks from exposure to
       thiobencarb in drinking water.                   . •     .

       v. Total Chronic Dietary (Food Sources and Water) Risk

             To assess total chronic dietary exposure and MOEs, the following formulas were
       utilized:                                          '   ;

       Total chronic dietary exposure = food source chronic exp. (average exp.) [mg/kg/day]
       (mg/kg/day)                      + drinking water exp.high end exp. *) [mg/kg/day] -
                                          27

-------
 % RfD =     total chronic dietary exposure Cmg/kg/day)
                                  RfD (mg/kg/day)

 * High end exposure was used due to a lack of average exposure data.

        Thus, this represents a percent RfD = 43.2%.  Therefore, EPA is not concerned
 With total chronic dietary risks from exposure to thiobencarb.

 vi. Dietary ("food source and water") Carcinogenic Risk
       Since  thiobencarb  is  a Group D carcinogen  (not  classifiable as to  human
carcinogenicity), a dietary carcinogenic risk assessment is not required.

              b.     Occupational Risk

i.  Handlers

       The short-term and intermediate-term MOEs for thiobencarb calculated from
Baseline,  additional personal protective  equipment, and engineering controls risk
Mitigation unit exposures are provided in Tables 8 and 9. The unit exposure values are
from PHED. An inhalation assessment was not undertaken, since no inhalation endpoint
of concern has been identified. Appendix 4 provides the  assumptions used for exposure
calculations.

       The daily dermal dose is calculated using a 60 kg body weight (average female
body weight) for short-term exposure, since this endpoint is based on a developmental
toxicity concern.  A 70 kg  body weight (average person bodyweight)  is used for
intermediate-term exposure. The following formula was used to calculate the daily dermal
dose:

Daily Dermal Dose (mg ai/kg/day) = Daily Dermal Exp. (mg ai/day) x I/Body weight (kg) x 60.2% dermal absorption
       These calculations of daily dermal dose of thiobencarb received by handlers are
used to assess the dermal risk to those handlers.  The short-term dermal MOEs were
calculated using a dermal absorption rate of 60.2 percent and a NOEL of 25 mg/kg/day.
The intermediate-term dermal MOEs were calculated using a dermal absorption rate of
60.2 percent and a NOEL of 2 mg/kg/day.  The following formula was used for MOE
calculations:

MOE = NOEL (mg/kg/day)/Daily Dermal Dose (mg/kg/day)
    •, : , iijilji'ijjIjIIIH • ; ;l  ,,i. i|	 n| 'NT ป ^ ป'.i i||in ;\ ,.(!   ' ,'|, ^H,',"'!' .P,, ';, lj li ',!!!!• 'Mij :|i.," • ; ,'i,W* "'i ;'"':i if > i" 	 • .iKii"!'!!,,,,1 ,'   , , ,,    ' , ,   '    ,  ,     , , ' „,

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       In general, for an endpoint,  EPA generally considers an MOE  of 100 to  be
protective of human health. The calculations of short-term risk estimates indicate.that the
                                    28

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       MOEs are greater than 100 at baseline (single-layer of clothing, chemical-resistant gloves,
       no engineering controls) for the following scenarios:

                     (2a) loading .granulars for fixed-wing aircraft application;
                     (2b) loading granulars for tractor drawn spreader application;"
                  >   (2b) applying granulars with a tractor drawn spreader;
                     (7)  applying sprays with a groundboom sprayer  (all rates);'and, '
                     (8)  flagging  liquid aerial application.

       The calculations of short-term risk estimates indicate that the MOEs are greater than 100
with additional PPE for (Ib) mixing/loading liquids for groundboom sprayer application.   .

              The calculations of short-term risk estimates indicate that the MOEs are
       than 100 with engineering controls  for the following scenarios:

              •      (la)  mixing/loading liquids for aerial application;
              •      (3)  applying liquid spray with a fixed-wing aircraft;
              •      (4)  applying granulars with a fixed-wing aircraft;
              •      (5)  applying liquid sprayer with a helicopter; and,

     1  Please note that at this, time, the Agency has insufficient data upon which to assess the
       exposures and risks to applicators in open cockpits.
                                           29

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NA: Not applicable since the previous MOE exceeded
None: No Engineering Controls exist for this scenario.
* Absorbed Dermal Dose (mg/kg/day) = (daily absorb
b Dermal Absorbed MOE = NOEL (2 mg/kg/day) / d<
Additional PPE:
la Double layer of clothing with chemical resistant glo
Ib Double layer of clothing with chemical resistant glo
2a Double layer of clothing with chemical resistant glo
6 Singel layer of clothing with chemical resistant glov<
7 Double layer of clothing with chemical resistant glo\
8 Double layer of clothing with out chemical resistant

-------
       i. Intermediate-term
     " The risks calculated from intermediate-term exposures to thiobencarb indicate that
risks from exposures to granular formulations (loading and applying) are lower than those
from exposures  to  the liquid formulation/spray  applications.    For  the  granular
formulations, the MOEs exceed 100 for all scenarios  (except loading to support aerial
application) with the addition of personal protective equipment. For many of the liquid
formulation/spray application  scenarios,  the MOEs do  not reach 100,  even with
engineering controls.

       Granular  Formulations:  The Agency  believes  the risks resulting from
intermediate-term exposures to the granular formulation are overestimated due to  the use
of the 60.2 percent dermal absorption value for the granular scenarios.  In general,  dermal
absorption of granular formulations  has been found to be significantly lower than for
liquid formulations.  Therefore, the Agency has determined that risks  to handlers of
granular formulations will be adequately mitigated with the addition of personal protective
equipment.                                              •
       liquid Formulations: The Agency believes that risks resulting from intermediate-
term exposures  to  persons  handling liquid  formulations are  overestimated due to
limitations with  the hazard  i^gg^Q^ an(j tne dose-response assessment for the
intermediate-term endpoint, particularly in light of the absence of serious effects to these
target organs in either the subchronic neurotoxicity or rat chronic feeding study, which
suggest the lack of a deleterious response to thiobencarb by the kidney and/or liver. The
Agency  Believes that "risks" to 1^31^ usingliquid formulations  will be adequately
mitigated with the used of engineering controls and personal protective equipment.

       ii.  Post-Application Workers

       Since no dislpdgeable foliar residue studies or concurrent dermal samples were
submitteS to me gpA for this MD to measure post-application reentry, a rough surrogate
post-application assessment was performed as a default.  Only the short-term endpoint (25
mg/kg/day) was used  for the risk assessment,  since  EPA does  not  anticipate  that
!mermeaiate:term exposures (i.e., 7 days or more of exposure)  are likely to occur for
post-application workers for these crops in early-stage development.  EPA assumed in the
surrogate ''assessment' "that "dermalabsorption would be significantly lower that the 60.2
percent used in me handler assessment, since dermal exposure would be to dry residues.
The surrogate post-application risk assessment indicated that at all application rates (i.e.,
4-8 pounds active ingredient per acre), risks would be acceptable to post-application
workers entering treated areas to perform tasks, such as scouting, thinning, or hoeing,
provided entry is postponed for 24 hours following application.
                                     32

-------
 was  based. on the parental/systemic .toxicity characterized  as  enlargement  of  the
 centrilobular heptocytes in both generations, hepatocyte single cell, necrosis observed in
 both sexes of both generations, and renal atrophic tubule consisting of regenerated
 epithelium. This NOEL is supported by the identical NOEL/LOEL established in the  90-
 day neurotoxicity study in rats; the  NOEL was 2 mg/kg/day and the LOEL was 20
 mg/kg/day based on systemic toxicity manifested as statistically significant increases in
 relative liver weights of male and kidney weights of female rats.

       For dermal absorption,  the TESC  determined the thiobencarb dermal absorption
 factor was 60.2% at 10 hours based on a rat dermal  absorption study.  This represents an
 upper bound estimate of absorption since the skin was washed approximately 1 hour prior
 to dosing rather than the recommended 24 hours.   However, if the study was repeated
 with the skin washed 24 hours  before treatment, the Agency anticipates a lower dermal.
 absorption factor for both thiobencarb formulations (i.e.  emulsifiable concentration and
 granular).

       Alternations in liver and kidney weights seen in the subchronic neurotoxicity study
•were corroborated with histopathological lesions in these organs in the multi-generation
 reproduction study  indicating  the liver  and  kidney to be target organs  following
 intermediate exposure  (i.e. up to several months) to thiobencarb-inducted toxicity at 20
 mg/kg/day.  However, the histopathological effects observed to  the liver and kidney  are
 not considered severe.  Instead, these  effects can be considered a detoxification effect to
 thiobencarb following an intermediate-term exposure.  The lack of renal or liver lesions
 in  the  rat chronic  feeding study provides support  that  the  target  organs'have a
 detoxification reaction to thiobencarb.     .     .       . •       •

              b.    Occupational Exposure

       In terms of occupational exposure, the unit exposure for mixing/loading liquids  for
 aerial application with a closed system (MOE =   18) is derived from four registrant
 submitted studies in PHED. There are  31 replicates for hand, 16 to 22 replicates for other
 dermal exposure, and 27 replicates for inhalation.  From the  31 replicates in the PHED,
 only 138 samples (approximately 4%) had non-detectable residues.  Dermal and inhalation
 exposure data are rated as high confidence based on the number of replicates and analytical
 grading criteria.  The Agency's confidence in the unit exposure values are high because
 exposures are based on detectable residues from high  confidence studies.  Since detectable
 residues were observed, normalizing residue estimates to half the level of quantification
 or half the level of detection to account  for non-detectable residues is not necessary. This
 provides additional support for the Agency's exposure estimates. In addition, most of  the
 dermal data are from studies involving whole body dosimeters,  which are generally a more
 accurate monitoring method than patch dosimetry.  The assumption of 350 and 80 acres
 treated for aerial and groundboom applications, respectively, are acceptable based on
 agricultural practices.
                                   . 34

-------
              Even though MOEs are less than 100 for several intermediate-term handler
       exposure scenarios,  the Agency believes that these risks  are  over-estimated due to
       limitations with the hazard identification and dose-response assessment.  The resulting
       histopathological lesions to the kidney and liver in the multi-generation study, and the
       absence of serious effects to these target organs in either the subchronic neurotoxicity or
       rat chronic feeding study, suggest the lack of a deleterious response of thiobencarb to the
       kidney and/or liver.  This effect, in  addition to the overestimate of dermal absorption,
       indicatej | a, COnse rvative, i estimate ,r of .intermediate-term handjer i risk.  Therefore,  the
       Agency concludes the intermediate-term handler MOEs are under-estimated and.are not
       at a risk of concern.
               II                  II                                       :
       C.    Environmental Assessment
              HI              '         mi         '            i         ,'.,,•.•     :• '•:;
              T,he environmental assessment consists of four sections: Ecological Toxicity Data;
       Environmental Fate  and Transport;  Ecological Exposure and  Risk Assessment; and
       Environmental Risk Characterization,  the first section reports ecological toxicity data
       from laboratory studies.   The  second section describes the environmental fate and
       transport data from field and laboratory studies, assesses the impact to water resources,
       and details the environmental fate assessment.  The third section estimates ecological
       exposure and assesses me effects to  non-target terrestrial  and aquatic organisms.  The
       fourth section, the Environmental Risk Characterization Section, integrates the exposure
       and effects assessment to determine the extent and potential for risk to the environment.

              1.     Ecological Toxicity Data

                     a.     Toxicity to Terrestrial Animals

                            (1)    Birds, Acute and Subacute

              An oral  (LDSO)  study  (preferably mallard or bobwhite quail) and two subacute
       dietary (LC50) studies (one species of waterfowl, preferably the mallard duck and one
       species of upland game bird,  preferably bobwhite quail)  are required to establish the
       toxicity of a pesticide to birds. Results of these tests are tabulated below.
 Table 10.  Avian Acute Oral Toxicity Findings (LDSO).
Species
Northern bobwhite
Northern bqbwhife
Mallard duck
% A.I.
96.9
Technical IMC 3950
r , Technical IMC 1 3950
LD5fl
(mg a.i,/kg)
>4938a
~
—
MRIDNo.
Author/Year
MRID 42600201
S.M. Campbell and
M.Jaber. 1992.
Ace. No. 095106
Ace. No. 095106
Toxicity
Category
Practically
nontoxic
-
-
fulfills Guideline
Requirement?
Yes
No, invalid1"
No, invalid11
'There were no mortalities in birds receiving a dose of 1938 mg ai/kg thiobencarb.
* This study wasperformed by; IndustrialBio-Test Laboratories, Inc.  Data.from all studies performed by this
laboratory are considered invalid and cannot provide any information for ecological risk assessments.
                                            35

-------
Table 11. Avian Subacute Dietary Toxicity Findings (LC50)
Specie?,
Northern bob-white
Northern bobwhite
Mallard duck
% AJ. :
"Technical"
"Technical"
"Technical"
lAo
(ppmai)
>,5620a
• --
, — •
MRIDNo.
Auflior/Year
Ace, No. 241483. 1979.
MRID 00057224 Ace; No. 095086
MRID 00057225 Ace. No. 095106
Toxicity
Category
Practically
nontoxic
—
—
Fulfills Guideline
Requirement?
No, supplemental
No, invalidb .
No, invalid1"
1 In this range-finding test for reproductive effects, there were no treatment-related mortalities in eight birds that were
fed a diet containing 5620 ppm for eight weeks.
b  This study was performed by Industrial Bio-Test Laboratories, Inc.  Data from all studies performed by this
laboratory are considered invalid and cannot provide any information for ecological risk assessments.

              These results indicate that thiobencarb is practically nontoxic to avian species on
       an acute oral basis. A supplemental study (Ace. No. 241483), which was designed to be
       a range-finding study for determining dose levels for a reproductive study, suggests that
       thiobencarb is probably also practically nontoxic to the bobwhite on a subacute dietary
       basis.  This conclusion is uncertain, though, since the study was not designed to  test
       subacute dietary toxicity and because only eight birds were used in the control and each
       test level rather than the recommended ten. The guideline requirements for the acute oral
       testing  [GLN 71-l(a)] are fulfilled.  The guideline requirements for subacute dietary
       testing [GLN 71-2(a and b)] are not fulfilled. The Agency requires that additional data
       be submitted on the subacute dietary toxicity to a waterfowl species, preferable the mallard
       duck [GLN 71-2(b)].  The Agency is not requesting additional testing with an upland game
       species [GLN 71-2(a)],  (MRID 42600601)

                            (2)    Birds, Chronic

              Avian reproduction  studies using the technical grade of the active ingredient
       (TGTA) are required when birds may be exposed to a pesticide repeatedly or continuously
       through its persistence, bioaccumulation, or from multiple applications, or if mammalian
       reproduction  tests indicate possible adverse reproductive effects.   The preferred  test
       species are the mallard duck and bobwhite quail. Avian reproduction studies are required
       for  thiobencarb  because  it  is  persistent in  the terrestrial environment  and  may
       bioaccumulate.  Results of these tests are tabulated below.
                                             36

-------
       study was 20 ppm (1 mg/kg/d).  In a reproductive study, parental rats receiving a dietary
       concentration of ,40 ppm (2 mg/kg/d) had histopathological effects on the liver (MRIDs
       40446201, 40985701).  As this was the lowest concentration tested, the NOEL was < 40
       ppm. Changes in body weights and mcreased 'kidney' weights were observed at 2000 ppm
       (100 mg/kg/d). No reproductive effects were observed at any test concentration, yielding
       a NOEL of  > 2000 ppm.  Based on these results,  20 ppm is considered to  be a
     . :conserya^e'l'NpEL for" effects'' oJtMoben'carb	on" wild "mammals.' (MRIDs 42130701,
     '' • 00154566, "40446261, "46985761)

                            (4)    Insects

              A honey bee  acute contact  LDSO study using the technical grade of the active
       ingredient is required when the proposed use will result in honey bee exposure. A honey
       bee acute contact study is not required for this pesticide because its use sites are not
       expected to result in significant exposure to bees.

                     b.      Toxicity to Aquatic Animals
"*"'" '   '•'•'"  '-  ":;	'••'••" "  	•''    (1)    Freshwater Fish, Acute

              Tfwb freshwater fish toxicity studies  using the technical  grade of the  active
       ingredient are required to establish the toxicity of'a pesticide to freshwater fish.  One study
       should use a coldwater species (preferably the rainbow trout), and .the other should use a
       watsiv^lie]: species (preferably the bluegill sunfish).   Results of these  tests are given
      "below.
::Table" l|. JEVesEyajerllsh Acute Toxicity Findings.
Species
Bluegill sunfish
Rainbow trout
Rainbow trout
Bluegill sunfish
Channel catfish
Bluegill sunfish
Carp
Bluegill sunfish
Rainbow trout
Channel catfish
/SBAi.'"
10*
10*
95.5
95.5
95.5
Technical
Technical
84.0b
84.0b
84.0b
••La*
(mg ai/L)
0.56
1.5
1.2
2.5
2.3
2.6
2.8
1.7
1.1
2.3
MRID No.
Author/Year
MRID 00050665, 1980
MRID 00050664, 1980
U.S.D.I., Ace. No. 095106, 1973.
U.S.D.I., Ace. No. 095106, 1973.
U.S.D.I., Ace. No. 095106, 1973.
Ace. No. 095106, 1974
Ace. No. 095106, 1974
U.S.D.I., Ace. No. 095106, 1973.
U.S.D.I., Access. No. 095106,
1973.
U.S.D.I., Access. No. 095106,
1973.
Toxjcity
Category
Highly toxic
Moderately toxic
Moderately toxic
Moderately toxic
Moderately toxic
Moderately toxic
Moderately toxic
Moderately toxic
Moderately toxic
Moderately toxic
Fulfills Guideline,
Requirement?
Yes, for TEP only
Yes, for TEP only
No, supplemental
No, supplemental
No, supplemental
No, supplemental
No, supplemental
No, supplemental
No, supplemental
No, supplemental
"Bolero 10 G
rBolero 8 EC
                                           38

-------
       The majority of the results indicate that thiobencarb is moderately toxic to fish on
 an acute basis.  The sole exception was an,acute test of bluegill sunfish exposed to Bolero
 10 G (10% ai) that determined the LC50 to be 0.56 ppm ai. This result is inconsistent with
 the results of two other acute tests which both determined that the LC50 for the bluegill
 sunfish was greater, in the range of 2.5 to 2.6 ppm ai.p. Results of tests with rainbow trout
 found that LC50's for this  species are slightly greater than 1, putting it in the moderately
 toxic range (> 1-10 ppm)'but close to the highly toxic range (0.1-1 ppm).  The Agency
.therefore concludes that thiobencarb is moderately to highly toxic to freshwater fish.

       The only fully acceptable studies  on the acute toxicity of thiobencarb to fish were
 conducted with Bolero 10 G.  These studies fulfill only the guideline requirements for
 testing with a TEP [GLN 72-1 (b) and 72-l(d)].   The guideline requirements for testing
 with the  technical grade  [GLN 72-l(a) and 72-l(c)] are not fulfilled by any particular
 studies, but the group of ten acute freshwater fish studies, when considered in its  entirety,
 is sufficient for fulfilling these guidelines.  (MRID 00050664 and 00050665, Ace. No
 095106).

                     (2)     Freshwater Fish, Chronic

      'A freshwater fish early life-stage test using the TGAI is required for thiobencarb
 because the end-use product may be applied directly to water or expected to be transported
 to water from the intended use site (rice) and because the following conditions  are met:
 (1) some  aquatic acute LC50 and EC50 are less than 1 mg/1,  (2) EECs in water (based on
 measured concentrations)  were greater than 1% of acute LC50 and  EC50 values,, and (3)
 the half-life in water is greater than 4 days.  No study with a freshwater fish species-has
•been submitted.  A study with a marine/estuarine  species (sheepshead minnow) was
 submitted (MRID 00079112), but this study does not fulfill the guideline because it failed
 to determine the NOEC.  The guideline for an early life-stage toxicity study with a fish
 species [GLN 72-4(a)3 has not been fulfilled.  However, the Agency does not request that
 the registrant  submit a study for this guideline.   Instead, the Agency requests that the
registrant  submit a core study that tests the effects of technical.thiobencarb over the life-
cycle of a fish
-------
 Table 14. Freshwater Invertebrate Acute Toxicity Findings.
1
Species
	 I * 1 1 '
Daphnid
Daphnia magna
Daphnid
Daphnia magna
Daphnid
Daphnia magna
Scud Gammarus
,4 ' i -,V- , 	 * If 	 ซ '"'•''
pseudolimlmaeus
Scud Gammarus^
pseudolimimaeus
Crayfish
Procambarus clariai
Apple snail
Pomacea aludosa
% AX
94.4
82.25*
10b
95.5
85*
95.5
85*
JLCJ& or ECjo
(pprn ai)
EC50= 0.10
EC50=0.17
EC50 = 0.46C
LC50 = 1.2
LCSO = 0.72
LC50= 1.0
LC50 = 2.0
LCSO = 1.85
MRIDNo.
Author/Year
MRID 00025788
Ace. No. 25788, 1978.
MRID 00079118
1980.
MRID 00050666
1980.
U.S.D.I., Ace. 095106,
1973.
U.S.D.I., Ace. 095106,
1973.
Ace. No. 095106
MRID 40031001
Toxicity
Category
Highly toxic
Highly toxic
Highly toxic
Highly toxic
Moderately
toxic
Moderately
toxic
Moderately
toxic
Fulfills Guideline
Requirement?
Yes
Yes, forTEP
No, supplemental
No, supplemental
No, supplemental
No, supplemental
No, supplemental
ปBokro8EC
*BolerolOG
The effect used to determine the EC30 was clumping of organisms.

              The results indicate that thiobencarb is highly toxic to aquatic invertebrates on an
       acute basis. The guidelme requirements for testing the XQAI [72-2(a)] and the TEP [72-
       2(b)] are fulfilled.  (Ace. No. 25788 and MRID 00079118).

                             (4)    Freshwater Invertebrate, Chronic

               An aquatic invertebrate life-cycle test using Daphnia magna using the TGAI is
       required for thiobencarb because the end-use product may be applied directly to water or
       expected  to be transported to water  from the primary use site (rice) and because the
       following conditions are met:  (1) some aquatic acute LC50's and ECso's are less than  1
       mg/1, (2) EECs in water (based on measured  concentrations) were greater than 1 % of
       acute LCjo and EC50 values, and (3) the half-life in water is greater than 4 days.  Daphnia
       magna is  the preferred test species.
 "fable 15.  Freshwater Invertebrate Chronic Toxicity Findings.
Species
Daphnid
Daphnia magna
Daphnid
Daphnia magna
I 	 ii IF| i '11 1"\ '
% A.L
I. ' i
95.2 - 95.9
96.9
NOE
1.7
66
MRIDNo.
Author/Year
Ace. No.
241483
42680401
Putt, 1993
Endpoints Affected
Reduced number of
young, adult mortality
Reduced number of
young
Fulfills Guideline
Requirement?
Yes '
No,
Supplemental1
1 This study is supplemental because measure concentrations were highly variable, the solvent was changed during the
PCS!, toฎ solvent in the solvent control was changed at a different time than in the test solutions, and chemical analysis
of the test material was not performed immediately after the solvents were changed.
                                             40

-------
       The core life-cycle toxicity study measuring the toxicity of thiobencarb to the
daphnid (Ace. No. 241483) indicates that thiobencarb concentrations as low as 3.0 Mg/L
can inhibit the reproduction in freshwater invertebrates.  The MATC of derived from this
study was  1.7 //g/L.  -This toxicity value was used for conducting the risk assessment
because it is the only core (i.e., fully acceptable) data available and it yielded the lowest
toxicity estimate.  A second life-cycle study with daphnid (M,RIP 42680401), which was
classified supplemental due to procedural deficiencies, yielded a greater toxicity estimate
(MATC=66 jug/L).

                     (5)    Estuarine and Marine Animals, Acute

       Acute toxicity testing ,with estuarine and marine organisms (fish, shrimp, and
oysters) using the technical grade of the active ingredient is required when an end-use
product is intended for direct application to the marine/estuarine environment or is
expected to reach this environment in significant concentrations. The  preferred test
organisms   are   the   sheepshead  minnow,   mysid   shrimp   and  eastern  oyster.
Estuarine/marine acute toxicity testing is required for this pesticide because its use on rice
is expected to result in  significant exposure to marine  and estuarine  environments.
Application of thiobencarb on rice fields will contaminate tailwater (i.e., water discharged
from the water management system) which may flow, into estuaries. The tables below
show the results of these tests for fish and aquatic invertebrates.         •  '  •
                                    41

-------
              Table 16. Acute Toxicity Findings for Marine/Estuarine Fish.
Species
1 • • • : • , •:'?•!,
Sheepshead minnow
Shecpshead minnow
Sheepshead minnow
Sheepsh' cad" minnow
Califqjtiift grunion
Leuresthes tenuis
(Static tests)
California grunion
Leuresthes tenuis
(Flow-through tests)
	 mi
Atlantic silverside
Menidia mcnidia
(Static tests)
Atlantic silverside
Menidia menidia
(Row-through tests)
Tidewater silverside
Media peninsulae
(Static tests)
Tidewater silverside
Media peninsulae
(How-through)
	 )ป:3S> ..;• -
	 A''T::

95.1
95.1
85.51
90
90
90
' 90 	 ^
i . h . " 	 i 	
" 90"",'
90
90
LC5o (ppm)
0.66
0.9
1.4
'"">" 0.9 	 "ll"'
0.31 (0 d old)
0.48 (7 d old)
0.59 (14 d old)
0.50 (28 d old)
0.27 (0 d old)
0.24 (7 d old)
0.38 (14 d old)
0.33 (28 d old)
0.46 (0 d old)
0.45 (7 d old)
0.63 (14 d old)
0.75 (28 d old)
0.39 (0 d old)
0.20 (7 d old)
0.41 (14 d old)
0.68 (28 d old)
633 	 (o'd'old)1"
0.40 (7 d old)
0.51 (14 d old)
1. 2 (28 d old)
0.30 (Od old)
0.46 (7 d old)
0.39 (14 d old)
0.82 (28 d old)
MRID No. Author/Year
MRID 00079112, 1979.
MRID 00079110, 1979.
MRID 00079111, 1979.
Borthwick and Walsh,
1981.
Borthwick etal., 1985.
ioxicny
Category
Highly toxic
Highly toxic
Moderately toxic
Not more than
"highly toxic"
Highly toxic
Highly toxic
Highly toxic
Highly toxic
Moderately to
highly toxic
Highly toxic
rumus umaeune
Requirement?
Yes
Yes
Yes, for TEP only
Open literature,
supplemental
Open literature,
supplemental
             "Bolero 8 EC
                            The results indicate that thiobencarb is highly toxic to marine/estuarine fish on an
                    acute basis. The guideline requirements for fish are fulfilled for the TGAI [GLN 72-3 (a)]
                    and for |TBP, Bolero 8 EC [GLN 72-3(d), MRID ].  (MRID 00079110, 00079111,and
                                '  '	'" ''"	"''
                                                           42
i •;,., Psiti:'jit a> •:. ti •,,	iigi:.;:,, : >	ij; •::, .1, Jth	t .1 .iillllL i liii;	I	l!	'il:.,	• :h.,i:-!H; .l;i!,:ii: t •:.	, li ..it!!!; ii ::iiilia^^^^^^^^^^^^^^^^^^^^	liailii	l!liซ^^^^^^^^^^^^     	iilllii	li^^^  	'A si	:,;..;j jii.:.:;

-------
Table 17.  Acute Toxicity Findings for Marine/Estuarine Invertebrates.
Species
Eastern oyster
(embryo-larvae)
Eastern oyster
(embryo-larvae)
Eastern oyster
(embryo-larvae)
Mysid shrimp
(<1 day old)
Mysid shrimp
(6-8 days old)
Mysid shrimp
(<1 day old)
Grass shrimp
Palaemonetes
pugio
Pink Shrimp
Penaeus duorarum
White Shrimp
Penaeus setiferus
Brown shrimp
Penaeus azetecus
Ghost shrimp
Fiddler crab
Shore crab
%AJ.
95.1
85.5a
90 •
94.6
95.1
90
85.5"
85.5"
"Tech.
ti
EC5D(ppra)
0.56
0.32
0.9 - 9.0
0.15
0.29
, 0.33
1.0 (adults),
0.38-0.57
(juveniles) .
0.57
0.31
0.47 \-
1.1
4.4
3.6
MRID No.
Author/Year
MRID 00079114, 1979.
MRID 00079115, 1979.
EPA-600/4-81-076,
Office of Research and
Development, 1981 .
MRID 00050667, 1980
MRID 00079117, 1979,
Borthwick and Walsh,
1981.
. Ace. No. 095106, 1975
MRID 00079113
Ace. No. 095106
Toxicity
Category
Highly toxic
Highly toxic
/
Moderately to
highly toxic
Highly toxic
Highly toxic
Highly toxic
Highly toxic
Highly toxic
Highly toxic
Highly toxic
Moderately
toxic
Moderately
toxic
Moderately
toxic
Fulfills Guideline
Requirement?
Yes
Yes, for TEP
Open literature,
supplemental
,Yes
No, supplemental
Open literature,
supplemental
No, supplemental
No, supplemental
No, supplemental
             The results indicate that thiobencarb is highly toxic to marine/estuarine mollusks
      on an acute basis. The guideline requirements for mollusks are fulfilled for the TGAI
      [GLN 72-3(b)] and for a TEP, Bolero 8 EC [GLN 72-3(e)]. The results indicate that
      thiobencarb is also highly toxic for marine/estuarine shrimp.  The guideline requirements
      for shrimp are fulfilled for the TGAI [GLN 72-3(c)]. (MRID 00079114, 00079115 and
      00050667).                                         ,

                          (6)     Estuarine and Marine Animals, Chronic

            Data from estuarine/marine fish early life-stage and aquatic invertebrate life-cycle
      toxicity tests  are  required if the product is applied directly to'the estuarine/marine
                                          43

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       environment or expected to be transported to this environment from the intended use site,
       and when any one of the following conditions exist: (1) the pesticide is intended for use
       such that its presence in water is likely to be continuous or recurrent regardless of toxicity;
       (2) any acute LC5Q or ECjp is less than 1 mg/L; (3) the EEC in water is equal to or greater
       than 1% of any acute EC50 or LQo value;  or (4) the actual or estimated environmental
       concentration in water resulting from use is less than 0.01  of any acute ECSO or LC50 value
       and any of the following conditions exist: studies  of other organisms indicate the
       reproductive physiology of fish and/or invertebrates may be affected, physicochemical
       properties indicate cumulative effects, or the pesticide has a half-life in water greater than
       4 days.  The preferred test organisms are the sheepshead minnow and mysid shrimp.

              Chronic testing with thiobencarb is required because it has a primary use (rice) for
       which It is applied directly to water or is applied to land which is subsequently flooded
       With water. In addition, concentrations of thiobencarb measured in aquatic field studies
       are as great as 0.085 ppin, which is greater man"O'.'bl of the LC50 for marine/estuarine fish'
       and aquatic invertebrates.  Results of this test are given below.
 Table 18. Estuarine/Marine Chronic Toxicity Findings
Species
1 i I '
Mysid
Grass
shrimp
Opossum
Shrimp
Mysid
J ' '"'1, ; ' V ,'! 1., '''.H '' '
Sheepshead
Minnow
% A.I.
95.1
84.7
"Technical"
Not reported
95.1
NOEC
(ppb)
ND,
EC05=
9,8b
<21C
3.2
22
ND
LOEC
)
ND
21C
6.2
, '.PI ii ||M h, .1 , „
35
150
MATC,
(ppb)
ND
<21C
4.5
28
<15Q
MRID No,
Author/Year
MRID 00079117
Ace. No.
241484, 1977.
MRID 43976801
Bailey, 1993
McKenney, 1985
1 f .' ,„ " :' '
MRID
00079112, 1979.
Endpatnfc?
Affected
Reproduction,
survival of
offspring
Adult mortality
Survival of
offspring
Number of
young produced
Wet weight
Fulfills
Guideline-
Requirement?
No,
supplemental
No,
supplemental
No,
supplemental
Open literature,
supplemental
No,
supplemental
* ND designates that the value was not determined.
ฅ The NOEC could not determined because the control had no replication. A nonlinear regression analysis (Bruce and
Vcrstecg, 1992) was used to calculate the ECOS which can be used in lieu of the NOEC.
* Levels are highly uncertain because measured concentrations were highly variable.
              The results indicate that a concentration of 150 ppb can adversely affect the growth
       of juvenile fish.  Concentrations less than 150 ppb may also have had adverse effects if
       they had been tested.   Because the study failed to  determine NCDECs, the guideline
       requirement for a fish early life-stage study  [72-4(a)]  has not been fulfilled.  (MRID
       00079112)
                                            44

-------
              The Agency does not request that the registrant repeat the fish early life-stage study
        [GLN 72-4(a)].  Instead, the registrant is required to submit a core study that tests the
        effects, of technical thiobencarb on the life-cycle of a fish (GLN 72-5).  The Agency is
       justified in requiring a fish life-cycle test because the end-use product is intended to be,
        applied directly to water or is expected to transport to water from the intended use site
        (rice), and because the EEC is greater than one-tenth of the NOEC in the invertebrate life-.
        cycle test.  This test should be conducted with a freshwater fish, preferable the fathead
        minnow or rainbow trout.  The Agency reserves the right to require a second fish life-
        cycle study using a saltwater species  at a later time.

              Chronic toxicity of thiobencarb to  crustaceans is uncertain because none of the
        studies submitted to the Agency was conducted in accordance to the guidelines for this
        study.   Based on supplemental data, the MATCs for crustaceans range, from 4.5 to 35
       ppb. Although no single study provided core data, the combination of supplemental data
        from four separate studies is sufficient to characterize the toxicity  of thiobencarb to
       marine/estuarine crustaceans.  The guideline requirement for life-cycle testing with a
        shrimp or mysid [GLN72-4(b)]  is thus fulfilled.  (MRID 00079117 and 43976801, Ace.
       No. 241484, McKenney,  1985)

                            (7)    Aquatic Field Studies

              The conclusion of high risk to aquatic organisms, based on results from laboratory
       toxicity tests, triggered the requirement for aquatic field testing with thiobencarb (GLN
       72-7).  The following aquatic field studies have been conducted on the use of thiobencarb
       on rice.       *                                                                     .

Table 19.  Aquatic field studies on thidbencarb on rice.
Title ' , > " ^
Studies in Halls Bayou to Test the
Effects of a Pre-Emergent Herbicide,
Bolero, on Aquatic Organisms
Impact of Bolero Runoff on a '
Brackish Water Ecosystem
Thiobencarb: 'Studies on Residue
Level and Behavior in Selected
Irrigation Creeks in Agricultural Areas
in Saga Prefecture, Southwestern
Japan
Locafioa and
Date
Halls Bayou/
Chocolate Bay,
Brazonia County,
Texas 1979
Matagorda,
Texas
1982 - 1984
Saga Prefecture,
Kyushu, Japan
1975-
Reference
Ace. No.
241484
MRDDs
92182086,
92182089
Ace. No.
241476
Performed By
Harper
Environmental
Consulting
Company
Biospheric,
Inc.
Life Science
Research
Institute
Sponsor
Chevron
Chemical
Company
Chevron
Chemical
Company
Unknown
Fulfills Guideline
Requirements'?
No, supplemental
Yes1
No, supplemental
  Following the review of this study, an additional aquatic field study was requested to monitor aquatic residues in-
other localities where rice is grown. This additional study, however, was waived in December 1993. No further field
studies are requested for thiobencarb at this time.
                                            45

-------
iir Mile	iii'ini11! liiri1!11 'j
              I'SIIMl"!	iriMll"!	IB	MrTU
                                             ffTfni	
                    iMilsJBayou_Study:  The first field study conducted in the U.S, was in rice  fields
                    bordering Halls Bayou, a tidally influenced, narrow stream that empties into West Bay
                    near Galvestpn,"Texas".  TMs"sta3y"Is also referred to as me Chocolate Bay study. This
                    estuarine  area is  a complex and  highly  important ecosystem  that  supports  many
                    commercial species.  .  Contaminated water was released into the bayou when rice fields
                    were irrigated with a.small amount of water (i.e. flushed) to moisten the soil. Also, heavy
                    rainfall  occurring   during  the  experiment resulted  in  two  additional  releases  of
                    contaminated water.  Sampling sites were  established 500 ft  downstream and 500 ft'
                    upstream of the point of discharge from the rice fields.  Water samples collected at the
                    field outlets and in Halls Bayou were analyzed for residues of thiobencarb. Fish, nektonic
                    macroinvertebrates, benthic organisms,  and phytoplanktpn were also sampled in these
                    areas before, during, and" after discharge'from'the nee fields.  Fish and macroinvertebrates
                    were also held in cages in Halls Bayou to monitor their response to the discharge of
                    thiobencarb.

                          Due to poor experimental design and experimental conditions that caused excessive
                    stress to the caged organisms, the Agency concluded that the results of the caged tests with
                    fish and shrimp were invalid. They thus yield no information which can be used for risk
                    assessment. Other  parts of the field study  provided some information and were thus
                    classified as supplemental

                          The highest concentrations of thiobencarb  were measured on a day when heavy
                    rainfall (3.23 inches) occurred on the same day that thiobencarb was applied, resulting in
                    an unscheduled flush overflow.  Peak; thiobencarb .concentra.ti.ons were 8.9 ppm (8900 ppb)
                    where the tailwater exited the rice field and 690 ppb  at the point, where the drainage water
                    entered Halls Bayou.  The highest concentrations  measured in the Halls Bayou on days
                    that were not associated with heavy rainfall  were 83 ppb at the upstream station (E) and
                    64 ppb at the downstream station (F).  The abundance of Ssh, invertebrates, and plankton
                    sampled at the downstream station were similar to or greater than those sampled at the
                    upstream station.  Gillnet catches declined  in only one of the two areas sampled after
                    discharges from the rice fields.  Seine and trawl sampling indicated a decline in abundance
                    of fish and invertebrates occurred near the end of the study.  All declines were observed
                    at both the upstream and downstream stations.  Some differences in species composition
                    of fish and invertebrates were observed between the upstream and downstream stations,
                    and some  changes in the species composition of benthic organisms were observed over
                    time.  None of these differences, however, could be conclusively linked to the discharge
                    of thiobencarb.
                          The biological findings of the Halls Bayou study were inconclusive since there
                    Were no significant differences in  species abundance or clear trends in the changes in
                    species composition between stations upstream and downstream of the point of discharge.
                    The upstream stations, being only 500 feet upstream of the site of discharge, were likely
                    close enough to be affected by contamination moving upstream  as the result of tidal
                    mixing.  Also,  the abundance and  composition of species were probably influenced by
                                                        46

-------
 other factors, including tidal cycles, salinity changes, and release of other pesticides from
 neighboring areas. Small samples sizes further limited the usefulness of this study.  This
 study does not provide much useful information on the effects  of thiobencarb on the
 estuarine environment.

 Matagorda Study:  A larger aquatic  field study was  conducted in 1982-1984 near
 Matagorda,, Texas. The site consisted of a rice field that drained through a ditch into the
 tidal waters of the lower Colorado River of eastern Texas. As with Hall's Bayou, this
 estuarine  area is a complex and  highly important ecosystem that supports  many
 commercial species.  No thiobencarb applications were made in 1982; this year provided
 baseline data for the site. Baseline thiobencarb concentrations were as high as 9 ppb.  In
 1983 and 1984, approximately 500 acres of the field were treated with thiobencarb at a
 rate of 4  Ibs ai  per acre.  Fields were flushed with water within 3  to 12  days  after
 application. Data collected from 1982 through 1984 included (1) residues of thiobencarb
 in water, sediment,  fish and shrimp; (2) catch per unit effort measurements of fish and
 aquatic invertebrates; and (3) percentages of grass shrimp (Palaemonetes pugid) that were
 gravid.  While samples were collected during all three years of the study, the sampling
 effort on the third year  was very poor.

       A control station was also  planned on  the Colorado River  upstream  of the
 confluence with the drainage ditch. However, during the course of the study, the Agency
 and the registrants agreed that this station could not serve as a control for the field study
 because it contained preexisting residues of thiobencarb.  It was therefore only possible
 to compare residues and biological samples collected during 1983 and 1984 to those
 collected during 1982, before the initial treatment.  This represents a shortcoming of this
 study since the results could have been influenced by yearly fluctuations in environmental
 conditions that are unrelated to the applications of thiobencarb. Another shortcoming is
 that other pesticides (ordram, basagran, machette, and propanil) were applied to  fields that
 drain into the test ditch  during the period of this study.  The toxicity of these pesticides
 could have contributed to the observed effects.                                       ,

       The results of the study were:

 1.     Residues of thiobencarb were transported into the estuary via runoff and drift.'
       Residues in water exceeded the aquatic invertebrate MATC  (1.7 ppb).  Maximum
       residues measured in water, sediment, fish, and shrimp were 25.1 ppb, 50 ppb,
       2400 ppb, and 970 ppb, respectively.

2.     Although  the overall population of fish was apparently  not affected, marked
       declines were observed during the  treatment years in  three species, Gambusia
       affinis, Dormitator maculatus, and Poecilia latipenna.
                                    47  .

-------
 3.     Several taxa of aquatic invertebrates showed substantial decline in numbers caught
        per unit effort.  Species richness and diversity also declined significantly during
        treatment years.

 4.     The percentage of gravid shrimp decreased significantly in 1983 compared to
        1982. The decline was aboutu 50%. at stations1and,2, and averaged 23 % for all
        four stations.  (Sampling was inadequate to assess the  effect on the percentage of
        gravid shrimp in 1984.)
 5.      ^)^of^&^hm&^ai^Mi0reypprtiapatronus) was observed in the area where
        the field runoff entered the drainage ditch. It occurred at the point of discharge
        from the drainage canal, one to two days after a post-application flush of the rice
        fields.  Although other pesticides that were applied that year (ordram, basagran,
        !|id propanil) may have been present in the tailwater,  this kill was attributed to
        thiobencaEb contamination because the dead fish contained  high residues  of
        thiobencarb (mean of 3.56 ppm).

 6.      Field BCF for thiobencarb were estimated to be 109X for fish and 44X for shrimp.

        Declines in fish, aquatic invertebrates, and gravid shrimp cannot conclusively  be
 attributed to the use of thiobencarb. Nevertheless, the findings in the field were consistent
 with effects demonstrated in laboratory studies.   They suggest that the application  of
 thiobencarb to rice fields may result in significant environmental damage to the adjacent
 estuarine habitat.  'Possible	effects^jnclude^chronic	effectsrto..sensitive fish, acute and
 chronic effects to ecologically ^important aquatic  invertebrates, chronic effects to grass
 shrimp and possibly to commercial shrimp, and indirect detrimental effects to organisms
 at higher trophic levels that depend on these organisms for food.

 Japan Study: The Agency reviewed a study that measured residues of thiobencarb in creek
 water after application to rice paddies in Japan. Thiobencarb was applied in the form of
 7% granules at a rate of 30 kg/ha, which is equivalent to 1.9 Ib ai/A. Water samples were
 taken from ten stations along creeks  that flow through the rice fields and drain into the
 Hayatsue River.  Water sampling was conducted from March through November, with
:thioBencarb tt^^iatsl.Bemg'madelfbm^ne'lS	^^g^ j^iy 2.' The creeks served as
 storage for irrigation water until May, when the water is  pumped onto the fields.  The
 creeics  resembleS large ponds during  the storage period.

        Very low thiobencarb concentrations (0.2 ppb or less) were reported at all stations
 in March and April before applications were made.  Concentrations peaked at the sampling
 period of My 1, when concentrations at most stations were between 20 and 40 ppb.  The
 greafest concentration was measured was 40.5 ppb.  Concentrations declined fairly rapidly
 thereafter- the half-life of thiobencarb  in creek water was estimated to be 8.8 days. This
 rate of decline represents dilution as well as biological and physical degradation processes.
 The Agency cannot interpret the significance of these results or extrapolate conclusions
                                     48

-------
to other areas because of the lack of important information on the test conditions, such as
flow rates within the creeks and rainfall during the study.

       A difficulty with all three of the field studies was that water flow measurements
were not made^ making it impossible to discern effects  of dissipation versus dilution.
While water residues were generally  short-lived, it is not clear whether thiobencarb
residues were broken down by chemical or biological forces, or they were swept away and
diluted ,by  tidal flow.   Because it is possible that dilution was the primary mode of
dissipation  in all three studies, the rate at which thiobencarb degrades by chemical or
biological means in estuaries remains unknown. Thiobencarb residues thus may persist
longer in other areas where dilution is of less importance in the dissipation of residues.

       The three biological field studies demonstrate that application of thiobencarb on
rice can- cause significant contamination to'water, sediments, and aquatic organisms in off-
site aquatic habitats..  Harm to estuarine and freshwater ecosystems is possible when
thiobencarb  is used in southeastern United States.  Although shortcomings of these studies
make it impossible to identify thiobencarb as the sole cause of observed adverse effects,
the studies  fail to refute the Agency's  presumption that the use of thiobencarb on rice
results in severe effects on aquatic ecosystems.

              c.      Toxicity  to Plants      .

                     (1)     Terrestrial

       Terrestrial plant testing (seedling emergence and vegetative vigor) is required for
herbicides which have terrestrial non-residential outdoor use patterns and which may move
off the application site through volatilization (vapor pressure _>_1.0 x 10"5mm Hg at 25ฐC)
or drift (aerial or irrigation),  and/or which may have endangered  or threatened plant
species associated with  the application site.  Terrestrial plant testing is required for
thiobencarb because it is an herbicide with a terrestrial nonresidential use pattern (rice) and
because aerial applications  may result in drift.

       For the seedling emergence and vegetative vigor testing the follqwing plant species
and groups should be tested: (1) six species of at least four dicotyledonous families, one
species of which is soybean (Glycine.max), and another of which is a root crop, and (2)
four species  of at least two monocoryledonous families, one species of which is corn (Zea
mays).
                                                                 v
       Results of Tier n seedling emergence toxicity testing on technical thiobencarb are
given below.  '                                   f                ,   „
                                    49

-------
                 ^	IIIIIH     	Jl	•:iiil
                                                                                       "it	S'fflT'v I*'!
Table 20. Nontarget Terrestrial Plant Seedling Emergence Toxicity Findings (Tier II).
Species
Monocot— Com
Monocot— Oat
Monocot— Onion
Monocot— Ryegrass
Dicot/Root Crop—Carrot
Djcpt-Cabbage
Dicot-Cucumber
Dicot-Lettuce
Dicot-Soybean
Dicot-Tomato
^ i'i jtป
%AL
96.6
Parameter
Affected
Shoot length
Shoot length
Shoot length
Mortality
Shoot length
Shoot length
Shoot length
Mortality
Shoot length
Shoot length
EC^
(Ib ai/A)
>1.7
0.086
2.0
0.01,9
>3.1
0.082
>1.7
0.27
>1.7
1.1
NOBC
(Ib ai/A)
1.7
0.055
0.94
0.00511
2.1
0.071
0.16
-
0.94
0.94
MRID No.
Author/Year
MRID 41690902
Hoberg, J.R.
1990
Fulfills Guideline
. + - - -i" * ! 5-H ^
Requirement?
Yes
Yes
Yes
Yes2
Yes
Yes
Yes
No, supplemental
Yes
Yes
1 This NOEL is based on 17% mortality of plants occurring at the next higher test level, 0.011 Ib ai/A.
2 Seedling emergence data for ryegrass is upgraded from supplemental to core.

              In the tier n seedling emergence test, mortality of test plants occurred in the tests
       with ryegrass, cabbage, and lettuce.  Mortality was the most sensitive toxic endpoint for
       these species (plants tended to  die shortly after emerging).   Grasses  appear to  be
       exceptionallysensitiveto tMobencarb.The  most sensitive  species was ryegrass, a
       monocot^ for which the EC^ based on mortality (i.e. LC25) was 0.019 Ib ai/A.  The most
       sensitive dicot was lettuce. The lettuce EC-^ based on mortality was estimated to be 0.27
       Ib ai/A, but this is not a definitive result since it was calculated from supplemental data.

              The guideline requirement  for  seedling emergence testing [123-1 (a)] is only
       partially fulfilled.  Core seedling emergence  data is outstanding for lettuce.   Lower
       dosages may need to be tested to determine the NOEC for this species. (MRID 41690902)

              Results of Tier II seedling  vegetative vigor toxicity  testing on  the  technical
       thiobencarb are given below.
Table 21. Nontarget Terrestrial Plant Vegetative Vigor Toxicity Findings (Tier II)
Species
Monocot- Corn
iiniiii
Monocot— Oat
Monocot— Onion
Monocot— Ryegrass
Dicot/ Root Crop-
Carrot
Dicot-Cabbage
Dicot— Cucumber
Diem—Lettuce
Dicot-Soybean
Dicot—Tomato
% AX

96.6
Parameter Affected
Shoot length, shoot
weight, and root weight
Shoot weight
Shoot length
Shoot length
Shoot length, shoot
weight, and root weight
Root weight
Shoot weight and root
weight
Root weight
Shoot weight
Root weight
ECซ
(Ib ai/A)
>2.2
0.17
1.2
0.073
>2.2
1.2
a
1.3 '
1.2
1.8
NOEC
Obai/A)
2.2
0.12
0.80
0.020
2.2
1.4
<0.12
0.80
0.80
2.2
MRID No.
.Author/Year
MRID 41690902
Hoberg, J.R.
1990
Fulfills Guideline
Requirement?
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
Yes
*Greater than a 25% reduction was recorded at some or all exposure levels, but the EC^ could not be determined
because no dose-response relationship was apparent.

-------
             In the Tier II vegetative vigor tests,  soybean was the most sensitive dicot and
      ryegrass was the most sensitive monocot. The guideline requirement for vegetative testing
      [123-l(b>] is fulfilled.  (MRID 41690902)

                           (2)    Aquatic

             Aquatic plant  testing  is required for any  herbicide which  has Outdoor non-
      residential terrestrial uses in which it may move off-site by runoff (solubility > 10 ppm
      in water), by drift (aerial or irrigation), or which is applied directly to  aquatic use sites
      (except residential). The following species should be tested:  Kirchneria subcapitata,
      Lemna gibba,  Skeletonema costatum,  Anabaenaflos-aquae, and a freshwater diatom.
      Aquatic plant testing is required for thiobencarb because it may be applied directly to
      water, it may be applied aerially, and it is applied to rice paddies where it is expected to
      contaminate the tailwater that leaves  the field.

             Results of Tier II toxicity testing on technical thiobencarb are given below.

Table 22. Nontarget Aquatic Plant Toxicity Findings (Tier II).
Species
Freshwater diatom
Navicula pelliculosa
Duckweed Lemna gibba
Green algae
Selenastrum capricomutum
Marine diatom
Skeletonema costatum
Blue-green algae
•Anabaena flos-aquae
Marine diatom
Skeletonema costatum
% A,L


96.6


95.5
EC,B
Cppb>
380
770
17
73
>3100
327-459a
NOBC
(ppb)
65
140
13
18
3100 .
_.
. MRID Nb.%
Author/Ye^r

*.
MRID 41690901 Giddings, J.M.
1990


EPA-600/4-81-076, Office of
Research and Development, 1981.
Fulfills Guideline
Requirement?
Yes
Yes
Yes
Yes
Yes
Open literature,
supplemental
        -50
             The Tier II results indicate that green algae is the most sensitive aquatic plant
      species. A thiobencarb concentration of 17 ppb ai is predicted to cause a 50% reduction
      in the growth and reproduction of this species.  The guideline requirement (123-2) is.
      fulfilled (MRID 41690901).

             2.     Environmental Fate

                   a.     Environmental Fate Assessment

             Thiobencarb is generally nonpersistent in the water  column  but  moderately
      persistent in soils and sediments.  Thiobencarb dissipates in the environment by binding
                                          51

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                     to soil, by aerobic soil metabolism at the soil/H2O interface, and by aqueous photolysis in
                     the presence of photosensitizers.  Ground water contamination is not likely from use on
                     the primary crop, rice, and surface water is not likely to receive significant amounts of
                     thiobencarb unless there is excess rainfall soon after application, leading to uncontrolled
                     runoff. When used on the rice, thiobencarb is more likely to be found in the soil than in
                     the paddy water.  Furthermore, greater quantities of thiobencarb are associated with soil
                     when appUed preflood to soilrather than.instanding water.  The parition of thiobencarb
                     associated with soil was approximately 10 times more when applied preflood to soil than
                     when applied to standing water, primarily since thiobe^^^
'	'' '"''"'  ""	  ;''  _f;, _;"  	Jio'floodmg.	As a result,_ sensitized aqueous photolysis is expected to be more significant
                     as a dissipation route when thiobencarb is applied to water than when it is applied to dry
             	 . a 
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       In two field studies in California where granules were applied into standing water,
the half-lives in flood water were 8.7 days (guideline study) and 4.5 days (literature
review,  Ross and Sava,. 1986).   The half-lives in hydrosol were 153  and 56 days,
respectively.  The median ratios of soil:water thiobencarb residues were 5.6:1 and 6.6:1.

       Thiobencarb moderately accumulated in  bluegill  sunfish   with maximum
bioconcentration factors of 128x, 639x, and 41 Ix for edible (muscle) tissue, nonedible
tissue, and whole fish, respectively.  Depuration is  rapid, with 93-95% of the accumulated
[14C]residues being  eliminated from the tissues in  three days.  The  degradates 4-
chlorobenzylmethylsulfoxide,  thiobencarb  sulfoxide,  desethylthiobencarb,  and  2-
hydroxythiobencarb were identified in edible and nonedible tissue. Based on results of
crop accumulation studies, thiobencarb does not appear to accumulate in plants.

             b.     Environmental Fate and Transport

                    (1)    Degradation

                           (a)     Hydrolysis

       Thiobencarb is stable1 to degradation by hydrolysis.  Thiobencarb did not degrade
in sterile aqueous buffer solutions (pH 5, 7,  and 9) that were incubated in darkness at 25
ฐC for 30 days.  The guideline requirement (GLN 161-1) is fulfilled.  (MRID 41609012)
                           (b)    Photodegradation
In Water
       Thiobencarb  photodegraded, with  a calculated  half-life  of 190  days  in a
nonsensitized sterile pH 7 aqueous buffer solution at 25 ฐC.  Photodegradation was more
rapid in a solution photosensitized with acetone with a half-life of 12 days. Thiobencarb
did not degrade in the dark control (non-sensitized).  The photoproducts identified in the
nonsensitized  and  sensitized  irradiated  solutions  were 4-chlorobenzoic  acid,  4-
chlorobenzaldehyde,      4-chlorobenzyl     alcohol,     and     N,N-diethyl-4-
(chlorobenzylthio)carbamate S-oxide (thiobencarb sulfoxide).   In the non-sensitized;./
irradiated  solution,  no photoproduct  exceeded 3.9  %  of the applied.   The  major
photoproducts  in   the  sensitized  solutions  were  4-chlorobenzoic  acid  and  4-
chlorobenzaldehyde, reaching  maximum  amounts  of  56 and 29.4 %  of  applied,
respectively. 4-Chlorobenzyl alcohol reached 6.1-6.7 % of applied by 14-30 days and
thiobencarb sulfoxide reached a maximum amount of 5  % by 14 days, and declined to  1..1
% by 30 days.  One  additional degradate, O-[(4-chlorophenyl)methyl]diethyl carbamate
(bencarb),  was isolated in the irradiated sensitized solution and reached 17.7 %  by 21
days, and declined to 12.4 % by 30 days.   The guideline requirement (GLN 161-2) is
fulfilled. (MRID 42257801)
                                    53

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On Soil

       Based on 30-day studies, thiobencarb slowly photodegraded on sandy loam soil,
irradiated under natural sunlight at Richmond, California with an extrapolated half-life of
168 days, and degraded in the dark controls with a calculated half-life of 280 days. In the
study, no volatile or non-volatile degradates exceeded 1.3 % of applied.  Non-extractable
residues did not exceed 8.7 % in the irradiated samples and 5.7 % in the dark control
samples by 26 days. The guideline requirement (161-3) is fulfilled., (MRID 41215312)

Photodegradation in Air

       No data were reviewed. This study was waived (4/29/91) because volatility is not
a significant dissipation route in the environment for thiobencarb.

                           (c)    Aerobic Soil Metabolism
       Thiobencarb  is moderately persistent in soils in California and Louisiana that
support rice production.  The calculated half-lives in three soils were 27-58 days in two
acceptable studies (MRID's  43300401, 00040925).  One supplemental study (MRID
431212Q1) provided additional information that supported the results of the two acceptable
studies.""'",,  , „„  ', ,  '"  ',, " '.' ""„',  "" „''„„'",'„"",'."'','.".,	.""	,	,",„'"   "	

       Thiobencarb appeared to degrade in a biphasic pattern with half-lives of 58 days
for 0-56 days after treatment and 137 days for 56-366 days in a Stockton Clay Adobe soil
from California (24 % sand, 30 % silt, 46 % clay, 2.2 % OC, pH 6.1).  The biphasic
pattern may be a result from thiobencarb binding to soils.  After the 56-day sampling
interval, the rate of degradation was significantly slower. There were six non-volatile
degradates detected in.the:  study, but none of;the degradates exceeded 5.4 % of the applied
dose (3.1 ppm).   The primary degradates were CO2, reaching 42.5 % of the applied by
the end of the study (366 days), and nonextractable residues, reaching 23.2 >% by the end
of the study.

       The reviewer-calculated half-lives in a clay soil from Biggs, California and a silty
day loam from Crowley, Louisiana were 37 and 27 days, respectively.  The clay soil (18
% sand,  26 % silt, 56 % clay, 1.13 % OC, pH 4.6, CEC 32.5 meq/lOOg) and the silty
clay loam from Crowley, LA (3 % sand, 69 % silt, 28 % clay, 0.79 % OC, pH 5.8, CEC
14P5 mga/iOOg) were representative soils from major rice growing regions of the U.S.
Evolved CO2 increased to 54-77 % by 1 year. The extractable, non-volatile degradates
did not exceed 5  % of applied, and bound residues increased to a maximum of 42 % of
applied by 1 year. (GLN 162-1, MRID 00040925)

       In a supplemental guideline study using a California soil, CO2 increased to 8.6 %
of applied by 132 days, and bound residues increased to 23.3 % of applied.  The soil was
a Stockton Clay Adobe (18 % sand, 27 % silt, 55 %  clay, 2.0  % OC, pH 6.0).  This was
                                    54

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intended to be an aged soil mobility study, but determination of meaningful Freundlich
coefficients was not possible due to the stability of thiobencarb.  Thiobencarb decreased
from 87.1 %  at time zero to 57.2  % by 132 days of incubation.  The calculated half-life
was 250  days.  Thiobencarb slowly mineralized in soil without forming significant
quantities of non-volatile degradates. (GLN 163-1, MRID 43121201)

                           (d)    Anaerobic Soil Metabolism

       Anaerobic  soil metabolism studies were not required because the registrant
submitted an anaerobic aquatic metabolism (GLN 162-3, MRID 00040925) instead.  (GLN
162-2, waived)

                           (e)     Anaerobic Aquatic Metabolism

       Thiobencarb is stable under anaerobic aquatic conditions. The registrant-calculated
half-life in sediment was 1962 days (5.4 years) (MRID 43252001).  In  supplemental
guideline studies, the registrant-calculated half-lives were 243 days in a sediment from
Louisiana and  >181  days in a sediment from California (Walker et al.,  1988).
Supplemental information  from  open  literature reported half-lives of 9-517 days in
sediment, and 31 and 82 days in  non-sterile and sterile water, respectively. The 9-day
half-life in sterile sediment reported in the literature study is not consistent with the other
data that show thiobencarb to be  more persistent in sterile test conditions-than in non-
sterile conditions.  The guideline (GLN  162-3) is fulfilled. (MRID 43252001)

       Anaerobic metabolism of  thiobencarb was measured in  clay sediment from the
Sacramento Valley (Stockton Clay Adobe,: 1.6 % sand, 32 %  silt, 52 % clay, 2.0  % OC,
pH 6.1) and water from the Sacramento River (pH 7.1, 44 mg/L alkalinity, total hardness
of 50.4 mg/L CaCO3).  The extrapolated half-life was 5.4 years (1962 days).  The
percentages of total thiobencarb residues in the sediment were 66.2 % at time zero, 76.6-
86.8 % from 7-272 days, and 65  % by 363 days.  Residues in water decreased from 20
% at time zero to 3.1-7.5 % from 7-272 days, and then increased to 23.3  % by 363 days.
Volatile residues did not exceed 0.9 % of applied. The degradate 4-chloro-benzoic acid
reached 14.'2 % of the radioactivity, in water at 70 days, which was only 0.3  %  of the
applied. It then decreased to 1.3-12.1  %  of the radiocarbon in water (<0.3  %  of the
applied).  No other degradate.reached 10 % of the radiocarbon in water. The guideline
requirement is fulfilled. (GLN 162-3, MRID 43252001)

       In a study that was considered supplemental because of deficient material balance,
the registrant-calculated half-lives were > 181 and 243 days in clay soil (Biggs, California,
18  % sand, 26 % silt, 56 % clay, 1.13  %  OC, pH 4.6, CEC 32.5 meq/lOOg) and silty
clay loam (Crowley, Louisiana, 3 % sand, 69 % silt, 28 % Clay, 0.79  % OC, pH 5.8,
CEC 14.5 meq/100g)/water systems, respectively. Non-volatile degradates and CO2 did
not exceed  3.8 .%,  indicating thiobencarb partitioned primarily into the  sediment.
                                    55

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       Unextracted residues increased to 42.8 % in the clay soil and 27.8 % in the silty clay loam
       by 364 days.  (GLN 162-3, MRID 00040925)

              Walker et al. (1988) determinedthe first order biotic and abiotic degradation rate
       constants for 14 pesticides (including thiobencarb) in estuarine water and sediment/water
       slurry systems (sterile and npn^sterile)""']	Trie half-lives in non-steriie and sterile sediment
       ranged from 9-517 days, fhe half-lives in sterile water and non-steriie water were 31.5
       and 82 days, respectively.  (Walker et 'al., 1988)
       i ,,i' ' ' i;   iiiiifiii I.;*1:,,, •< , ii  " •, "''• i ,„ ,ni	>: \ i	\ H. n iii"',,i,'iiMir1:1  ,i 'i'1" '.V' A:, ,  I'^'Wiii. v'LiJh1, ',': • '?.• \'t MI: ik'j I'l';"", ' 'i •  , ,.• "- ,1  ','„,ป   •     ' i •"
              Chen et al. (1982) created a model aquatic ecosystem and applied 14C-thiobencarb
       to determine its partitioning in the laboratory environment. It was not possible to calculate
       a half-life because of limited sampling.   By the end of the experiment  (23 days),
       thiobencarb partitioned  mostiy into sand (23.2 % of applied) and to a lesser extent into
       water aid biota (2.7 and 0.31  % of applied, respectively) . The authors attributed the low
       recovery of radioactivity to volatility, photodecomposition, and microbial decomposition.
       (Chenetal., 1982)

                                   (f)    Aerobic Aquatic Metabolism

              Thiobencarb was  stable to aerpbic aquatic metabolism in a clay soil/water system
       from the rice-growing area of California.  The"'guideline requirement (162-4) is fulfilled.
       (MRID 42015301)

                            (2) Mobility

       Unaged Mobility (Batch  Equilibrium)

              Thiobencarb was moderately mobile to immobile in five soils.  Freundlich K^
       values ranged from 5.4 to 20.1 in the tested soils, and Koc's ranged from 384 to 1435 (see
       below Table).  The unaged portion of the guideline requirement (GLN 163-1) is fulfilled.
       (MRID 41215313)

Table 23.  Results of aged mobility studies with thiobencarb.
Sofl Texture
:"(%OQ ; ";:;;";
Sandy Loam (0.5)
Loam (1.9)
Silty Clay (1.5)
Clay Loam (1.1)
SUt Loam (1.4)
Freundlich
Kjj,
5.4
7.3
9.3
11.3
20.1
Freundlich
Koc^
1084
384
618
1027
1435
Freundlich Kte
14.3
21.7
28.8
46.7
94.5
Freundlich
Koc*.
2860
1142
1920
4245
6750
.N (slope values) for
adsorption and desorption
0.8, 1.0
1.1, 1.1
1.1, 1.1
1.2, 1.2
1.0, 1.1
       Aged Mobility

       Based on batch equilibrium experiments, the degradate 4-chlorobenzoic acid was very
mobile to moderately mobile in the tested soils with Freundlich Kads of 0.7-3.3 (See below Table).
                                            56

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Mobility generally decreased with increasing clay content, increasing organic matter content, and
increasing cation exchange capacity. The aged portion of the guideline requirement (GLN 163-1)
is fulfilled.  (MRID 43150601)

           I          ' '                      ' '       .  S
Table 24. Results of aged mobility studies with thiobencarb
Soil Texture {% QC) .
Sandy Loam (0.88)
Loam (0.76)
Silt Loam (0.88)
Clay (2.0)
Ereundlich
K.ab
0.74
1.0
1.2
3.3
Freundlich
Kocaas t
84
130
140
160
Freundlich
^•to
2.2-
1.9'
2.4
8.3
Freuadlich
'Koc4K
250
250
280
420
N (slope values) for
adsarptipu and desorption
1.6, 1.6
1.6, 1.5 -
1.6, 1.6
1.3, 1.2
       Laboratory and Field Volatility           .

             Volatility testing was waived (4/29/91) since volatility is not a significant means
       of dissipation of thiobencarb.  (GLNs 163-2 and 163-3, waived, see also GLN 161-4)

                           (3)  Accumulation

       Accumulation in Irrigated Crops                  .

             Thiobencarb was detected (detection limit of 0.07 ppm) in the tops,of table beets
       grown in plots of clay soil in California that were sprinkler-irrigated five times at 8- to 13-\
       day intervals with  water containing  thiobencarb  (Bolero  8 EC,  85 %  emulsifiable
       concentrate) at approximately 200 ppb.'  Thiobencarb was not detected (detection limit of
       0.01 ppm) in either the beet foot or in tomato fruits grown under similar conditions.  In
       addition,  the potential degradate 4-chlorobenzylmethyl sulfone was not detected in beet
       tops or roots, or in tomato fruits.   In the 0- to 6-inch depth of the treated soil,, parent
       thiobencarb and the degradate thiobencarb sulfoxide were 0.04-0.13 and _<_0.02 ppm,
       respectively, at all sampling intervals. There was no apparent pattern of accumulation or
       decline of either parent thiobencarb or the degradate.  (GLN 165-3, MRID 43148201)  .

       Bioaccumulation in fish                    •

             Thiobencarb residues  accumulated  in juvenile  bluegill sunfish exposed  to
       [14C]thiobencarb at 0.05 mg/L, with maximum bioconcentration factors of 128x, 639x,
       and.411x for edible (muscle) tissue, nonedible tissue, and whole fish, respectively. The
       degradates 4-chlorobenzylmethylsulfoxide, thiobencarb sulfoxide, desethylthiobencarb,
       and 2-hydroxythiobencarb were identified in edible and nonedible tissue.  By day 3 of the
       depuration period, 93-95% of the  accumulated [14C]residu.es were eliminated from the
       tissues. The guideline requirement (GLN 165-4) is fulfilled. (MRID 42460401)
                                          57

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              c.      Field Dissipation

                            (a)    Terrestrial Field Dissipation

       The registrant has submitted sufficient information on terrestrial field dissipation
(164-1) to do an environmental fate assessment for the 40,000 acres of vegetables in
Florida.  Considering the small acreage of this use, the aquatic field dissipation study for
rice in Louisiana provided adequate information on the fate of thiobencarb under terrestrial
conditions, in addition to aquatic conditions. Therefore, terrestrial field dissipation data
are reserved for any future terrestrial uses of thiobencarb.

                            (b)    Aquatic Field Dissipation
        III              III      III      II           I        	  ;   • '    •  •  ,  . ' , -"
       In two field studies in California where granules were applied into standing water,
the half-lives in water were 8.7 days in the guideline study (MRID 43404005)  and 4.5
days in the literature study (Ross and Sava, 1986).  The soil half-lives determined in the
two studies were 153 and 56  days, respectively. The median amounts of thiobencarb in
soil were 5.6 and 6.6 times higher than in water,  respectively. No leaching was observed
below 6 inches of depth. GLN. 164-1, MRID 42003404.  The guideline is only partially
satisfied since the registrant  did not provide storage stability of samples and since the
movement of water in the CA guideline study  (MRID 43404005) was not described in
detail.

       Thiobencarb dissipated with an observed  half-life of approximately 6 days in silty
clay loam soil in Louisiana that had been planted to rice. The plot was flooded at 7 days
postfreainent; thiobencarb dissipated from the floodwater with a registrant-calculated half-
life of 5.8	days.	Thiobencarb^ was not detected in the	soil below 10 centimeters.  The
degradates  l-(((4-cMorophenyl)memyl)sulfonyl)-N,N-diemyiformamide  (thiobencarb
sulfoxide) and 4-chlorobenzyl-methylsulfone were detected primarily in the upper 5 cm
of the soil and in the floodwater.  (MRID 42003404)

       Thiobencarb (10 G) was applied in one application by air at 4 Ibs ai/A to flooded
plots of Anita clay loam (28 % sand, 26 % silt, 46 % clay, 2.47 % OC, pH 6.1,  CEC of
46). Soil cores were taken to 30 cm (1 foot) of depth throughout the study at 0-551 days
after treatment. There was one 8-foot core taken at 153  days, which was divided into
segments ranging from 5 cm at the surface to 30 cm at lower depths.  Water samples were
taken at 0-92 days after treatment.  Water samples were also collected from the fallow
field replicates from days  15-21 and day 27.

       The half-life in soil was 20 days for the 0-92 day (flooded) sampling periods, and
was 153 days when all sampling intervals (0-551 days) were considered. The half-life in
water was 4.8 days when the 0-33  day sampling intervals were considered.  (MRID
43404005)
                                    58

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        Ross and Sava (1986) studied two commercial rice fields in the Sacramento Valley.
 of CA.  Thiobencarb was applied at 4 Ibs ai/A using fixed-wing aircraft into standing
 water when rice plants had not yet emerged (1-3 leaf growth stage). Water was held at
 10.4 inches of depth for 6 days with no inflow or outflow (stagnant water).  After 6 days,
 the field was rapidly drained to 6.8 inches of depth with intermittent inflow and outflow!.
 Water temperatures averaged 28 ฐC (82ฐ.F) for 30 days.  Water,  soil, and vegetation
 samples were collected from four pads within each rice field.  The pads were located at
 the field inlet and outlet and two randomly-chosen points in between.  Samples were taken
 at -1, 0, 2, 4, 8, 16, and 32 days after application near the pads and where the water flow
 was slower.  The dynamics of herbicide dissipation were examined using a split plot
 analysis or variance (ANOVA). Air, water, soil, and vegetation were analyzed using GC.

        Thiobencarb was predominantly distributed between water (34.5 %) and soil (43
 %),  with less than  1%  associated with  air  and  vegetation.    Thiobencarb water
 concentrations at 0, 2, 4, 6, 8, 16, and 32 days after treatment were 79. 567, 576, 515,
 367, 56, and 8 mg/L, respectively. Soil concentrations of thiobencarb were 3250  288o'
 3350,  3860, 2020,  2260, and  2330 /zg/kg  (ppb),  respectively.   Thiobencarb  air
 concentrations atO, 1, 2, and 3 days after treatment were 1.4, 0.9, 0.8 and 0.43 mg/m3,
 respectively.; The calculated half-life in air was 2.2 days. The evaporative flux rates were
 37, 8, 16, and 6 ng/cm2 h"1  at 0, 1, 2,  and 3 days'after  treatment, respectively.
 Thiobencarb vegetative concentrations were 78,  691,  1750,  1360,  1280,  796 and 169
 mg/kg ,(ppb),  respectively, leading to a calculated half-life of 8,5 days using, natural
 logarithm data. Concentrations in water, soil, and vegetation were significantly higher in
 the holding period than in the postholding period.  Water and vegetation concentrations
 were stable in the holding period and only declined with time during the postholding
 period.  In contrast, soil concentrations did not change during either period. The mass
 balance (including air, water,  soil, and vegetation) increased from 41 % at 0 days after
 treatment to 67-70  % by 2-6 days after treatment and then decreased to 26-27 % by 16-32
 days after treatment.                                                 '

       The guideline requirement (GLN 164-2) is only partially fulfilled. This guideline
 can fulfilled if the field, dissipation study conducted in Louisiana (MRID 42003404) is-
 upgraded by the submittal of more detailed information on the water management used at
 the study site and the storage stability of the test samples.  (GLN 164-2, MRID 41722504  '
 42003404, 43404005).

                     (1)    Spray  Drift  (Droplet   Size   Spectrum/Drift  Field
                           Evaluation)

       No thiobencarb specific studies were reviewed.  Droplet size spectrum (GLN 201-
 1) and drift field evaluation (GLN 202-1) studies are required for thiobencarb, since the
different formulations may be applied by aircraft and it is estimated that there will be
detrimental effects to non-target terrestrial and semi-aquatic plants due to drift.  However,
to satisfy these requirements the registrant, in conjunction with other registrants of other
                                    59

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pesticide active ingredients, formed the Spray Drift Task Force (SDTF). The SDTF has
completed and submitted to the Agency its series of studies  which are intended to
characterize spray droplet drift potential due to various factors, including application
methods, application equipment, meteorological conditions, crop geometry, and droplet
characteristics. During 1996 the Agency plans to  evaluate these studies. In the interim,
and for this assessment of thiobencarb, the Agency is relying on previously submitted
spray drift data and the open literature for off-target drift rates. The estimated drift rates
at 100 feet downwind of the treated sites are 1 % at the applied spray volume from ground
applications and 5% from aerial applications. After review of the new studies the Agency
will determine whether a reassessment is warranted of the potential risks of the application
of thiobencarb products.

             d.      Water Resources

                     (1)    Ground Water

       The Office of Pesticide Programs (OPP) evaluates the persistence and mobility of
eac.h pesticide for ground water concerns.  If the data indicate that the parent  and/or
degradates are persistent and mobile, then a small-scale prospective ground water study
may be requested.  The basic triggering criteria include: weight of the evidence from
laboratory and field dissipation studies indicating that the pesticide has properties  and
characteristics similar to pesticides that are known to  leach or have been detected in
ground water; movement of the parent or degradates 75-90 cm through the soil profile or
plow iayer in a field dissipation study; reports of dTetecSons in ground water from other
mQn|tQr|ng studies  and information about toxicity. In addition, use patterns, application
rates, timing of application, potential acreage treated, depth to ground water, soil types,
hydraulic gradient, and climate are also evaluated as part of the triggering criteria.
                                                                !
       Persistence, mobility, and detections in ground water are also used to evaluate a
chemical to determine whether its use should be restricted for ground water concerns. A
pesticide may be recommended as a candidate for restriction if it exceeds one or more
criteria for each of the three factors (persistence, mobility, and detections).

                           (a)     Persistence and Mobility

       Thiobencarb was evaluated for persistence and mobility in relation to its potential
to leach to ground  water.  Below is  a summary of that evaluation.
                                    60

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Table 24. Mobility and Persistence of Thiobencarb Relative to Restricted Use Criteria
Factor
Persistence
Mobility

1
2
' 3
4
5
6
7
Characteristic
Field dissipation half-life
Lab-derived aerobic soil
metabolism half-life
Hydrolysis half-life
Photolysis half-life (Soil)
Soil adsorption: Kd
Soil adsorption: K^
Depth of leaching in
field dissipation study
Restricted Use
- Criteria
> 3 weeks
> 3 weeks
< 10% in 30 days
< 10% in 30 days
< or = 5 ml/g
< or = 500 ml/g
75 cm
"" Reported Values* ,
8, 21.9 weeks (56, 153 days)b
3.9 -8.3 weeks (27 - 58 days)
Stable
50% in 168 days (calculated)
5.4 -20.1 ml/g .
384,618, 1024 - 1435 ml/g • ,
15cmb
b Because thiobencarb is used almost exclusively on rice, no terrestrial field dissipation studies were submitted by the
registrant.  Aquatic field dissipation studies were conducted to address this use.  The half-lives reported are aquatic- field
dissipation for soil.  Refer to Section C.2.b. of the Agency RED chapter for additional data.

                                    (b)    Degradates and Binding

              The aerobic  metabolism studies found that, after one year, the degradation to
       carbon dioxide and binding of residues to soil  were significant pathways for dissipation
       of thiobencarb (see Section C.2.b.). Carbon dioxide accounted for 42-77% of the applied
       and bound residues accounted for 23-42% of the  applied.  Literature data reported that the
       thiobencarb in the soil slowly mineralized without forming significant quantities of non-
       volatile degradates. This will significantly reduce the amount of thiobencarb available to
       leach through the soil profile.                                        ,        •

                                   (c)    Ground Water Detections

              The Agency has limited monitoring information for thiobencarb in ground water
       in the United States. The "Pesticides in Ground Water Database" (Hoheisel et al.,  1992)
       reports sampling for thiobencarb in 270 wells in  California and 65 wells in Missouri.
       Two detections of thiobencarb in ground water were reported in Missouri, these were very
       low (0.2 - 0.3 ppb).  A summary of this is presented below.
Criterion
Detections
Detections
Characteristic
Number of wells per state with detections
Number of counties with detections >
10 % of reference point
Restricted tfse Criteria
25 wells in 4 or more states or
3 counties at > 10% of MCL or
HAL
.Reported Detections
2 wells in 1 state'
No MCL or HA
Established
                                            61

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"I":1  "   :'' '   "I1.""."."	.'.."	'.!!'.'	(d)    Restricted Use

       Thiobencarb met the persistence and mobility triggers for classification as a restricted use
 chemical for ground-water concerns, but not the detections triggers.  The Agency believes that
 ground water concerns do not warrant use restrictions.

                                  (e)     Ground Water Reference Points

       There is no MCL established for thiobencarb residues in drinking water. The lifetime
 Health Advisory for thiobencarb  also has not yet been established, but an estimated Health
 Advisory can be calculated from the Reference Dose.  The Agency has established the RfD of
 thiobencarb at 0.01 mg/kg/day.

       The Agency estimated the lifetime HA for thiobencarb to be 70 ppb.  This was calculated
 from the Reference Dose as follows:

       Assumed:     Adult with.body weight of;701 kg consuming 2 L water/day
                     RfD  for thiobencarb...=. 0,01 mg/kg/day
                     RSC = Relative source contribution, assumed to be 20%

       DWEL = (RfD) ("70 kg) = (0.01 mg/kg/day) x 70 kg  = 0.35 mg/L
                      (2L/d)            (2L/d)
       Lifetime HA = DWEL x RSC = 0.35 mg/L x 0.20 =  0.07 mg/L = 70 ฃtg/L (ppb)

                                  (f)     Ground Water Concerns

              Thiobencarb is slightly persistent in water, generally not very mobile, tends to bind
       to soil organic matter, and doesn't desorb.  The Agency has estimated the Lifetime Health
       Advisory for thiobencarb residues in drinking water to be 70 ppb. Thiobencarb also has
       low acute '^mammalian toxicity.  Based on the limited  data available and very low
       concentrations found in  ground  water, there  is no  indication that thiobencarb
       concentrations in ground water would exceed the estimated HA of 70 ppb.

              The principle use of thiobencarb is rice in the lower Mississippi Valley and the
       Central Valley of California. Rice fields are usually underlain by a clay layer to restrict
       water movement through the soil and help contain the water in the flooded field. This clay
       layer will significantly limit the amount of leaching that occurs in the rice fields.
              Although thiobencarb does exceed several of the criteria for restricted use, the
       Agency does not consider thiobencarb to be a candidate for restricted use due to ground
       water concerns. The Agency does not consider thiobencarb to be a concern in ground
       wafer, nor a human health concern from residues in drinking water that are derived from
       ground water.
                                           62

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                            (2)    Surface Water
               Environmental fate information indicates thiobencarb is. nonrpersistent4 in the
        water column (aquatic field dissipation half-lives ranging from approximately 6 to 9 days).
        It is stable to degradation from abiotic hydrolysis; however, degradation via aqueous
        photolysis with photosensitizes was shown in the laboratory studies.  The vapor pressure
        and, Henry's Law constant.indicate thiobencarb will not volatilize readily from surface
        water environments.  Based on the FreundlicTi adsorption coefficients (Kads range: 5.42-2(3
        ml/g),  thiobencarb  adsorbs  to soil  and sediment particles and may be transported on
        entrained sediment in surface runoff.  Partitioning of thiobencarb onto soil or sediment
        was  demonstrated  in  three  aquatic  field dissipation  studies  where,  thiobencarb
        concentrations on soil were approximately 5 to 64 times greater than water concentrations.
        However, the results of field monitoring studies indicate thiobencarb can be transported
        primarily via dissolution in runoff water if sufficient rainfall occurs immediately foUowing
        field application.  In surface waters, thiobencarb dissipates  principally by binding to
        sediment, and degrading by sensitized aqueous photolysis.  Mineralization is also known
        to  occur at  the  soil-water interface  in  rice fields.   This is aerobic  degradation.
        Thiobencarb has the potential to contaminate surface water from releases of rice paddy
        water which closely follow field application,  or from spray drift associated with aerial or
        ground spray application.

              Thiobencarb is  not  currently regulated  under the Safe Drinking Water Act
        (SDWA); therefore, a Maximum Contaminant Level (MCL) is not established.   It is
        classified as category m  for oral acute toxicity. The estimated .lifetime Health Advisory
        level (HA) is 70 mg/L using the Reference Dose of 0.01  mg/kg/day.  Public water supply
        systems are not required to sample and analyze for thiobenearb.

              In the EPA Office of Water's STORET database, thiobencarb detections in surface
        waters were reported for .filtered water samples only (detections  limits varied from 0.002-
        0.008 mg/L).  Detections qf thiobencarb were listed'for 8 states: California, Georgia,
       Maryland, North Carolina, Oregon, Oklahoma, Texas, and Washington.5   Thirty-nine
       positive detections were reported for 3,130 samples (approximately 1 %) with a maximum:'-':-'X-
        concentration of 0.24 mg/L (7/22/92 and  8/26/92; Klamath Falls, OR) and a mean-'?
       concentration of 0.10 mg/L. Whole (i.e., unfiltered) water samples did not find detectable . •
       levels of thiobencarb. Surface water concentrations for the field monitoring studies are
        several orders of magnitude  greater (approximately 100 to 3000 times larger) 6than the
       detections reported in the, STORET database.  The sources of this variation are not known;
   4 Based on the criteria established by McEwen and Stephenson (1979,),                                  ''.•".

    The Agency does not know why thiobencarb was detected in these states since, with the exception of California',: ''.• • !X'"'•".:""
registered uses of thiobencarb should not occur within these states.                                  '   '" "'"''   '
                                           63

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 however, the filtered sample results (0.7 mm filters) in the STORET data suggest very low
 concentrations of thibb'encafb in the aqueous phase of surface water samples. This finding
 is consistent with the partitioning of'thibbencarH onto sediment which would lower the
 concenfiations in the aqueous phase.  It is not clear whether the results from the field
 monitoring studies were determined from filtered or unfiltered water samples.  Additional
 surface water monitoring data are described in Section 3a(2)(b).

        Aquatic EEC modeling  for rice uses  was not conducted because the Agency
 currently  does not gave  a" computer  simulation"  model which will estimate these
 concentrations.  For the lettuce, endive and celery uses, the GENEEC model was used to
 complete a Tier 1 exposure assessment  (Table I). The range of aquatic EECs was 140
 mg/L for the 6 Ib a.i. application rate and 180 mg/L for the 8 Ib a.i. application rate.  The
 ,^,g^l ^^.gg^—v— ฃ-..ฃ j^Qj-Q-y'^g—yj- for eac'Epound increase in thiobencarb.
 Comparison of the initial EECs with the 21-day and 56-day EECs indicates thiobencarb
 dissipates in the pond water at an approximate rate of 0.4-0.6 mg/L/day.

        Increased  transport time to  the water intakes  will  allow greater  binding  of
 thiobencarb to suspended solids and/or sediments. Any thiobencarb that may reach surface
 water  will  be  predominantly bound with suspended solids  and sediments.   Standard
 coagulation and flocculation processes used in these plants should remove most of the
 suspended solids an"3 'sediments ""frbm""the water |" thereby removing most of the potential
 risk of thipbencarb in drinldng water.	'  	  i	'

        3.     Exposure and Risk Characterization

               a.     Ecological Exposure and Risk Characterization

                      (1)     Background Information
        III 111  I           ^1      I   II   I     l   ||   ||    ||    '.,..:,,'     •   '. •":    • .; .

         Risk Quotients and the Levels of Concern

         Levels of Concern (LOCs) are criteria used to indicate potential risk to nontarget
  organisms.  Exceeding the criteria indicate that a pesticide, when used as directed,  has the
 potential to cause undesirable effects to nontarget organisms.   Two general categories of
  LOG  (acute and chronic) exist  for each of the four nontarget fauna! groups, and one
 category (acute) exists for each of two nontarget floral groups. To determine if an LOG
 has been exceeded, a risk quotient is derived and compared to the LOG.  A risk quotient
 is  calculated  by   dividing  an  appropriate   exposure  estimate,  e.g.  the estimated
  environmental concentration (EEC), by the appropriate toxicity test effect level.  The
  acute effect levels are:                                                           ;

•:!':,""'• •:••'*!-I  ':':•   -r EC^'(terrestrialplants)	
               .-?+ EC50 (aquatic plants and invertebrates)
                — LC50 (fish and birds)
                                      64  ...
                                   rVr	li1 >,'!', -.fit",	I!1!;! , "iriHM

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                    ~ LD50 (birds and mammals)
                    ~ ECOS or NOEC (endangered plants)
             The chronic effect levels are the:                                       '

                    — NOEC (avian and mammal reproduction studies)
                    — NOEC or MATC for aquatic species.

             When the RQ exceeds the LOG for a particular category, risk is presumed.  Risk
       presumptions, along with the corresponding LOCs, are tabulated below.

 Table 26. Risk Quotients and LOCs for Animals
Endpoinr
Risk Quotient (RQ)
LOCT
Birds
Acute High Risk
Acute Restricted Use
Acute Endangered Species
Chronic High Risk
Chronic Endangered Species
EEC/LC50 or LD50/sq. ft or LD50/day
EEC/LCSO or LD50/sq. ft or LDso/day (or LD50 < 50
mg/kg)
EEC/LC50 or LD50/sq. ft LDso/day
EEC/NOEC
EEC/NOEC
0.5
0.2
- 0.1
1
1
' " Wild Mammals
Acute High Risk
Acute Restricted Use
Acute Endangered Species
EEC/LCSO or LD50/sq. ft or LD50/day
EEC/LCSO or LD50/sq. ft or LD50/day (or LD50 < 50
mg/kg) ,
EEC/LCSO or LD50/sq. ft or LD50/day
Aquatic Animals
Acute High Risk
Acute Restricted Use
Acute Endangered Species
Chronic High Risk
Chronic Endangered Species
EEC/LCSO or EC50
EEC/LCSO or ECSO ,
EEC/LC50 or EC50
EEC/MATC or NOEC
EEC/MATCorNOEC
0.5
0.2 .
0.1

0.5
0:1
0.05 •
1
1
Table 27. Risk Quotients and LOCs for Plants
Endpoint
.Risk Quotient {.KQ)
Terrestrial and Semi-Aquatic Plants
Acute Plants
Acute Endangered Species
EEC/EC^
EEC/EC05 or NOEC
Aquatic Plants
Acute Plants
Acute Eridangered Species
•EEC/EC50,
EEC/EC05 or NOEC . ' '
LOG
••
1
. 1

1
1
                                         65

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                                                                               •	I.,'.'''„'ซ" ' IIL	:|ii
       At this time, The Agency has no procedures for assessing chronic risk to plants,
acute or chronic risks to nontarget insects, or chronic risk from granular/bait formulations
to mammalian or avian species.

       Thiobencarb Use Patterns Addressed in Risk Assessment

       The majority of thiobencarb use (95%) is to control terrestrial and aquatic weeds
in rice production. The maximum label use rate on rice is 4 Ib ai/A, and the average rate
is approximately 3 Ib ai/A.  Application may be by aircraft or ground equipment.  For
rice grown in the Gulf Coast and Mississippi River Valley, thiobencarb is usually applied
as a liquid (EC formulation) to nonflooded fields.  "Dry-seeded"  rice is frequently grown
in this area, in  which seeds are sowed and grown in dry seed beds for several weeks
before flooding.  If there is no rainfall, fields are irrigated with  a small volume of water .
(i.e. flushed) to'promoteseed germination.  Some rice in this area is "water-seeded",
meaning that seeds are applied to water in flooded fields.  In this part of the country,
thiobencarb is usually applied to fields before they are flooded. Fields are then flooded
for seeding with rice. These  floods are normally dropped temporarily after seeding to
allow rice seedlings to grow, resulting in a discharge of water.  In California, the majority
of rice grown is water-seeded with a continuous flood.  Unlike the southern region,
thiobencarb in California is almost always applied as a granule to water in flooded fields.
A small percentage of rice farmers in California use "piii-point flood" culture, in which
case thiobencarb may be applied as a liquid to dry-ground before fields are flooded.
California state  regulations prevent rice farmers from discharging tailwater  from rice
fields for 4 to 30 days after application.

       Additionally,  a relatively small amount of thiobencarb is used on lettuce, endive,
and celery.  Registrations for these uses are restricted to Florida.  The maximum label rate
is 6 Ib  ai/A for lettuce  and endive "and 8 Ib ai/A for celery   Application is by boom
sprayers.         	'"""	"	"'  	'

                     (2)    Exposure and Risk to Nontarget Terrestrial Animals

                           (a)    Birds

       Liquid Applications- Acute and Chronic Risk

       For thiobencarb products applied as a liquid to soil, risk is assessed by comparing
LG;o v?!16? to estimated residues  (i.e. EECs) on dietary food items immediately following
application.  The table below  giv&faepi^cied'Q^iay maximum and mean residues of
thiobencarb that are expected to occur on selected avian or mammalian dietary food items.
                                     66

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  Table 28. Maximum EECs on Avian and Mammalian Food Items for Uses of Thiobencarb.
Use Site ' \
Rice
Lettuce and Endive
Celery
Maximum Application
- Rate(lbsa.i./A)
4
'6
.- 8
..Maximum. EEC (ppm)
Short gras$
960
1440
1920
.Long grass
440
660
880
Broadleaf. plants and insects
540
810
1080
Fruit
60
90
120
          •  ,  In an avian dietary LC50 test with the northern bobwhite (Ace. No. 241483), no
       mortality occurred at the maximum test level, 5620. ppm.  Environmental concentrations
       are predicted to be much less than 5620 ppb. The acute risk to birds from all uses of
       thiobencarb is  minimal.  No acute effects to threatened and endangered species are
       expected.                                      ,

              The chronic risk quotients for liquid applications are given below.

Table 29. Avian Chronic Risk Quotients (RQs) for Liquid Applications Based on a
Mallard Duck NOEC and Maximum EECs.
Crop
Rice
Lettuce and
Endive
Celery '
Maximum-
Application Rate
(Ibs a-i/A) -
4
6
8
Pood Items
Short grass
Long grass
Broadleaf plants and insects
Fruit
Short grass
Long grass
Broadleaf plants and insects
Fruit '••
Short grass
Long grass
Broadleaf plants and insects
Fruif
Maximum
EEC
(ppm)' I
960
440
540
60 .
1440
660
, 810
90
1920
880
1,080
120
NOEC
{ppm)"
100
100
100
100
100
100
100
100
100
100 .
100
100
Chronic RQ.
(EECVNOEC)
9.6
4.4
5.40
0.6
14.4
6.6
8.10
0.9
19.2
8.8
10.80
1.2
Number of
Bays EEC
> NOEC
29
19
, 21
0
34
24
26
0
37
27
30
, 3
              In addition to the magnitude of the RQs, chronic risk can be assessed by estimating
       the duration .when EECs are expected to be high enough to possibly cause effects in birds.
       This duration is based on the magnitude of the initial EEC and the rate-of dissipation. The
       dissipation of thiobencarb from foliage was estimated from data collected in a biological .
       field study conducted at Halls Bayou, Texas (Ace..No. 241484). Thiobencarb residues,
       were measured from broadleaf weeds and sedges collected 12 m downwind of the edge of
       the field on 0, 7, 14,  and 21 days after application. The calculated foliage half-lives for
       broadleaf weeds and sedges were 5.4  and 8.6 days, respectively.  These  values are
                                           67

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 consistent with those estimated for other pesticides (Willis and McDowell, 1987).  The
 more protective value of 8.6 days was used in the risk assessment.  Assuming a first-order
 rate of dissipation, EECs are predicted to exceed the> mallard NOEC for up to 37 days,
 depending on the use rate and type of plant.
•" '     • "™'  ",  :!! '„:,'                        $'              : ' •       ,
        Most of the chronic risk quotients exceed the LOG of 1 for use on rice, lettuce,
 endive, and celery.  The use rate would have to be reduced to 0.4 Ib/A,  one-tenth the
 currentpaximum label rate for rice, to reduce all of the chronic RQs to below,the LOG.
 Furthermore,  the maximum'BECs'for''all food'types except fruit  exceed the mallard
 NOEC for relatively long durations, generally three weeks or more.  These results indicate
 that all uses of thiobencarb pose a risk of causing chronic effects to birds and may cause
 chronic adverse effects to threatened and endangered bird species.

 Granular Applications—Acute Risk

        A granular formulation is used only when thiobencarb is applied to flooded rice
 fields.  Most of the granules will fall onto the water surface and sink to the bottom. These
 granules would not b"e accessible to many birds, although they possibly could be ingested
 by waterfowl and sandpipers which feed off the bottoms of the flooded fields. A small
 portion of the granules may fall on levees built around and within rice fields  or get caught
 in emerging rice plants.  These granules could be available for birds to consume. Some
 exposure of granular pesticides to birds is therefore expected, but the overall degree of
 exposure is probably less than when thiobencarb is applied on dry fields.

       iThiobencarb^has, very low acute foxicity to birds.. In an acute single-dose test with
 the northern bobwhite, a dose of 1938 mg ai/kg Bwt resulted in no mortality or overt signs
 of toxicity (MRID 42600201).  The Agency expects that the risk of acute effects to birds
 from exposure to granular thiobencarb is minimal.

 Granular Applications—Chronic Risks

        The Agency currently does not have a procedure for assessing the chronic risk
 posed by granular applications.

                            (b)    Mammals
        ii                                      i       i       • , •   .       '' •' .
       Acute hazard to small mammals was addressed using the acute oral LD50 value for
 the rat converted to an estimated LC50 value for dietary exposure. The estimated LC50 was
 derived using the following formula:

        LC50 = LD50 x body weight (g) / food consumed per day (g)
                                     68

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              Acute risk to mammals was assessed by calculating RQs for three representative
       species: the meadow vole, the field mouse, and the least shrew.  Estimated mammalian
       LC50 values for these three species of small mammals are presented below:

Table 30.  Estimated Small Mammal Dietary Exposure (Based oh an LDSO =  1080 mg/kg).
Small Mammal \
Meadow vole
Adult field mouse
Least shrew
Body Weight '(g)
46
13
5
Percent of Weight Eaten
Per Day
61 %
16 %
110 %
Pood Consumed i
Per Day 
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Table 32. Mammalian Chronic Risk Quotients (RQs) for Liquid Applications Based on a Rat NOEC
and Maximum EECs.
Crop
   Maximum
Application Rate
  (Ibsa.i./A) ;
Food Items
Maximum
  EEC
  
   NOEC
                         Short grass
                                         960
                                   20
                     48
                        48
Rice
                         Long grass
                                         440
                                   20
                     22
                        38
                         Broadleaf plants and insects
                                         540
                                   20
                     27
                        41
                         Fruit
                                          60
                                   20
                                  14
Lettuce
and
Endive
                         Short grass
                                         1440
                                   20
                     72
               Long grass
                          660
           20
           33
                43
               Broadleaf plants and insects
                          810
           20
           41
                46
                         Fruit
                                          90
                                   20
                                  19
                         Short grass
                                         1920
                                   20
                     96
                        57
Celery
                         Long grass
                                         880
                                   20
                     44
                        47
                         Broadleaf plants and insects
                                         1080
                                   20
                     54
                        50
                         Fruit
                                         120
                                   20
                                 22
             All of the chronic RQ for mammals exceed the LOG of 1 for use on rice, lettuce,
      endive, and celery. Except for exposure on fruit, all RQs are very high (22 or greater).
      Also, EECs on wildlife foods are expected to persist at levels greater than the mammalian
      NOEC for many days.  These results indicate that all uses of thiobencarb pose a risk of
      causing chronic effects to mammals and may cause chronic adverse effects to threatened
      and endangered species of mammals.

             The specific responses of the tested organisms in the study yielding the 20 ppm
      NOEL were reduced weight gain and food consumption, food efficiency, and increased
      blood urea nitrogen  at 100 ppm, the next highest test level above 20 ppm.  In another
      study, no reproductive effects were observed at dietary concentrations as high as 2000
      ppm. Thus the chronic risk to mammals relate to growth and physiology. Available data
      do not suggest high risk of reproductive impairment to mammals at any application level.

      Granular—Acute Risks

             A granular formulation is used  only when thiobencarb is applied to flooded rice
      fields. Most of the granules will fall onto the water surface and sink to the bottom. These
      granules probably would not be accessible to most mammals.  The active ingredient of
     ',,!!,'	ill,!1 i . , ,"n i "'P. II Mllii;	Illf", ,ihi,l, ,'f 	I	''Ik,, I1	1	 "Ill',I	'IIHI II'IMIIII	„	I III ,'i, 'i"	'ill,,	'"	,lii, 	 I,' ,,,! 	,,	II il	  'I,' 	'	 'II, I'I'	  ill ,     " , "  I , I
      these granules would disperse into the water, and mammals could then be exposed by
      drinking""this water.   But, considering the large degree of dilution that would take place
      and the  low mammalian tpxicity of "tibdbbehcafb," this would pose minimal risk to
      mammals.   The primary route of exposure to  mammals from granular thiobencarb
      probably would be from ingestion of granules that fall on the levees within and around the
              ",11!-*!!
             • ir.K	

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      To be protective, the values were selected to maximize calculated exposure estimates.

             GEECs based on runoff from a single application on a 10-hectare field to a 1-
      hectare x 2-meter deep water body are given below.
Table 33.  Generic Estimated Environmental Concentrations (GEECs) for Aquatic Exposure.
Use Site
Celery (FL)
Lettuce and
Endive (FL)
Application Method
Ground, unincorporated
Ground, unincorporated
Maximum Application
Rate(}bsa.L/A) \
8
6
Number of
Applications
1
1 .
Initial (Peak)
EEC(pp-b) !
186
140
21-day EEC
)
173
130
56-day EEC
fepb)
157
118
                                 (b)    Measured Aquatic Concentrations

             The Agency used aquatic concentrations measured in field studies and monitoring
      projects to estimate the exposure of aquatic organisms from use of thiobencarb on rice.
      Measured water concentrations were available from two biological field studies performed
      in Texas (MRID 92182086 and 92182089, Ace. No. 241484), two environmental fate field
      studies performed  in Louisiana and  California (MRID 42003404 and  43404005),
      monitoring data.from California (MacCoy et al., 1995, MRID 43359700), and studies
      reported in the open literature (Ross and  Sava, 1986; Watanabe et al., 1982). While data
      from all of these sources were reviewed, the risk assessment for dry-seeded rice in the
      Southeast was based primarily on data from the,two biological field studies and the risk
      .assessment for water-seeded rice in California was  based on data from the monitoring
      programs.   These  sources  were  most  relevant  because  they  provided measured
      concentrations of thiobencarb residues in off-site bodies of water, rather than in the rice
      fields themselves.

             Dry-Seeded Rice in the Southeast

             The two biological field studies provide examples of residues that can result in
      slow-moving bodies of water that receive drainage from surrounding dry-seeded rice fields
      where thiobencarb is applied.  The Agency considers both Halls Bayou and the canal
      studied near Matagorda, Texas to be representative of small brackish waterways that occur
      in the rice-growing region of the Gulf Coast.  These field studies will also be used in the
      risk assessment to represent freshwater  habitats of the Mississippi Valley since no study
      specific to this region is  available.  The 1978 Stream Evaluation Map for the state of
      Texas categorizes the section of Hall's Bayou  where the field study was located as
      Category  I,  a "highest-valued fishery resource".  The drainage ditch in the Matagorda
      study was a 120-130 ft wide permanently flooded canal that is also considered to be a
      biologically significant habitat. Biological sampling in both studies found these waterways
      are abundant and diverse in fish and invertebrate life.

            The greatest exposure from dry-seeded rice culture probably results from the first
      flush or heavy rainfall that occurs after thiobencarb has been applied.  Pesticide residues
                                          72

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in the soil are dissolved in this water as it passes over the field and then discharged into
an aquatic habitat.  As demonstrated in the Hall's Bayou field study, unplanned flushes
resulting&6in''fairtfall" usually resultiri greater residues'in off-site aquatic habitats than do
planned flushes.  Planned flushes usually occur one to two weeks  after application,
allowing much of the pesticide to bind to the soil.  Furthermore, since rice farmers
normally try to use the minimal amount of water required to adequately  wet the soil, little
water is normally discharged in a planned flush.  On the other hand, rainfall may occur
soon after the pesticide is applied, and if it is intense, may result in a large volume of
water beiTig discharged from'the'field.

       The biological field study that collected water samples from Halls Bayou, Texas
[see section C.l.b(7)], provides an example of a near worst-case scenario. . Halls Bayou
is a long narrow waterway that meanders through rice-growing areas in the Texas coastal
plane.  During the field  study, a very heavy rainfall event (3.23 inches in 24 hours)
happened to occurred on the same day that thiobencarb was applied, resulting in unplanned
release of water from rice fields. The measured concentrations of thiobencarb in samples
taken this day were as  great as 8900 ppb in the field outflow and 690 ppb at the point
Where a ditch draining from the fields discharged into Hall's Bayou. Heavy rainfall of this
magnitude is common in the Gulf Coast region, often occurring several times in the same
location during  the spring.  However, the Agency believes  that these thiobencarb
concentrations represent near the upper bound of concentrations that are likely to occur
in aquatic habitats  for the following reasons:  1) rainfall occurred on the same day  that
pesticide is applied;  2) in the area of the field study.  Halls Bayou was surrounded by  rice
fields; and 3)galls 'Bayou isa" relatively•"small" w"a|e^^y "With" "little flow or tidal flushing,
resulting in little dilution of water  draining from rice fields.

       The aquatic residues measured at other times during the Halls Bayou study, as well
as those  measured in  a  drainage ditch in the biological field study  conducted near
Matagorda, Texas [see section C.l.b(7)], provide examples of situations that would result
in exposures that are moderately high,  put more typical. Other  than the concentrations
measured after the intense rainfall event of the 19th of April, the greatest thiobencarb
concentrations were measured in samples taken on 7 April following  several planned
flushes in the region during the previous week.  Samples taken that day at two sites in the
Bayou near the point of discharge  of water draining from the rice fields were 83 and 64
ppb.  Measured residues were 40 and 48 ppb in two samples taken on 6 April, and 33  ppb
in a sample taken on 8 April. Residues remained at a concentration of 10 ppb or greater
through April 12, when sampling was ceased.

       Aquatic residues  measured in the study near Matagorda study were less than those
measured at Halls Bayou.  The greatest measured residues (average of two replicate
samples) was 20 ppb in I983""and"21'ppb in 1984.  Residues were commonly between 1
and 15 ppb for a few days following applications of thiobencarb aid/or flushing of fields.
                                     73

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        regions.   Unlike in California, thiobencarb is primarily applied to water-seeded rice in
        these regions by applying a liquid to soil before the fields are flooded.  This use is similar
        to dry-seeded rice in terms  of expected environmental exposure.   Therefore,  the
        concentrations measured for dry-seeded rice in the biological field studies was also used
        to represent exposures from water-seeded rice in the southeastern regions.

        Water-seeded Rice in California

               The Agency used surface water monitoring data to approximate the concentration
        of thiobencarb likely to be found in water in areas of'California where water-seeded rice
        is grown.  Two recent sources of data on thiobencarb were available.  One was a US
        Geological  Survey  Open-file  Report  on  the  monitoring   of  dissolved pesticide
        concentrations in the San Joaquin River and Sacramento River in  1991 through 1993
        (MacCoy et  al., 1995).   The other source of data, was  monitoring  of  thiobencarb
        concentrations in waterways that drain rice growing areas in the Sacramento River Valley.
        This data was submitted to the EPA by Valent U.S.A. Corporation under the 6(a)(2)
        provisions of FIFRA.

               Approximately three years of routine  water monitoring of the San Joaquin River
        at VernaliSj California and of the Sacramento River at Sacramento, California (MacCoy
        et al., 1995) found that thiobencarb concentrations were always low, below 0.05 ppb.  For
        most of the sampling period, thiobencarb residues were not detected or were below 0.025
        ppb.  The only exception  was a 10-12 period during May and June of 1993 when
        measured residues, generally were greater than 0.1 ppb. Peak residues were 0.53 ppb in
        the San Juaquin River and 0.70 ppb  in the Sacramento River.
                                                                     /
               Monitoring conducted by the California Environmental Protection Agency and
        submitted by the Valent U.S.A.  Company found that levels of thiobencarb in some of the
        waterways draining into the Sacramento River were occasionally greater than in the river
        itself.  Concentrations were always below 1 ppb in the Butte Slough, but frequently
        greater than 1 ppb in the Colusa Basin Drain (Table 34). A peak concentration of 37 ppb
        was recorded in the Colusa  Basin Drain.
Table 34. Thiobencarb concentrations measured in waterways flowing into the Sacramento River,
California                                                                            '
Date Sampled
5-3-94
5-5-94
5-9-94
5-12-94
5-16-94
Water Sampling Locations
CBD1
—
—
,_
r-
0.212
CBD5
, not detected
0.18
0.49
0.42
37.4
SSI
•,
—
— '
—
0.34
BS1
o.io :
0.09
0.06
0.07
0.11
                                           75

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Date Sampled
5-19-94
5-22-94
5-26-94
5-30-94
6-2-94
$$•$4 	 '."".'
-94 	
6:13,-94 ..
6-16-94
6-20-94
6-23-94
6-27-94
4:30-94 	 , 	
7-4-94
7-7-94
Water Sampling Locations
CBD1
0.103
3.34
0.8
0.46
0.28
0.58
15.8
6,2
4-74

	 — 	
— •
	 — 	 	
-
	 — 	
CBD5
0.768
',. ,:,/^0^,r" "..,..:
;': 	 ; ; 0.9*92 	
0.66
4
0.5
0.38
0.34
0.284
0.42
0.11
0.51
0.63
0.28
0.21
SSI
0.40
0.26
0.19
0.12
nd
0.10
0.08
0.08
0.10 .
—

—
i" '
—
/
BS1
0.09
0.08
0.11
0.18
0.12
nd
0.10
nd
0.08
nd
0.53
nd
nd
0.12
0.18
(nd •ป not detected)
       Vegetables in Southern Florida

             Very limited information, is available on thiobencarb concentrations in southern
       Florida.  The National Water Quality Assessment Program (NAWQA), being conducted
       by the US Geological Survey, is currently collecting water samples in southern Florida
       and analyzing them for pesticides, including thiobencarb. One sampling station is located
       in an area that is drained by agricultural land in Palm Beach County where most of the
       Florida use of thiobencarb is located. This Station is on the HUlsborro canal along the
       southern border of the Loxahatchee  National Wildlife Refuge, and thus would provide
       indication if thiobencarb is entering this important wetland habitat.

             To date, testing for thiobencarb has only been completed for approximately 16
       Samples that were taken in the fall of 1996 into the winter of 1997. Thiobencarb was not
       detected in these samples.  The minimum detection level of thiobencarb is 0.002 /^g/L.
       However, it is premature to draw any conclusions from these data.  For one thing, these
       data do  not yet include sampling  for the spring, the time when most applications of
       thiobencarb are made. Interpretation of the NAWQA monitoring data should be postponed
       until the collection, quality control, and analysis of the data have been completed.
                                          76

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                            (c)    Freshwater Fish

       The risk of acute effects to freshwater fish from the use of thiobencarb on rice is
uncertain.  The maximum residue measured in Halls Bayou when heavy rainfall occurred
on the day .of application was 690 ppb.  This exceeded the lowest LC50 determined for
freshwater fish, 560 ppb for the bluegill sunfish, indicating a high risk to freshwater fish.
This conclusion is supported by a fish killed observed in the Matagorda study which was
attributable to thiobencarb exposure.  Several other supplemental studies, however, found
that the LC50 for various freshwater fish is greater, in the range of 1.1 to 2.8 ppm. Based
on these supplemental data, the acute risk of thiobencarb would be minimum to  low.
Furthermore, other aquatic residues measured in the Halls Bayou, as well as all of those
measured in the Matagorda study, were well below even the lowest LG50  of 560 ppb.
Therefore, the acute risk of thiobencarb to fish is uncertain.  If there is an acute risk, it
apparently would be limited to high exposure situations in,which heavy rainfall occurs
soon after application discharges contaminated water into a small water body where it
would not be greatly diluted.

       Measured aquatic residues from monitoring in California were no greater than 37
ppb.   As  this is  well below levels  that are expected to cause acute effects, use of
thiobencarb on rice in California is expected to pose minimal acute risk to freshwater fish.
No acute effects on threatened and endangered species are expected.

       For other uses of thiobencarb, acute risk quotients for freshwater fish are given
Table 35.                                                         .
Use Site
Celery (FL)
Lettuce and endive (FL)
• Use Rate (LB/ A)
8
6
kC5fr (ppb)
560
560
EEC Initial 
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       The maximum residue measured in Halls Bayou after a heavy rainfall was 690 ppb,
which was 6.9 times greater than the acute EC50 for Daphnia magna, 100 ppb. Residues
measured in Halls Bayou on another day that was not associated with a heavy rainfall
event wife 83 and 6,4 ppb.  Risk quotients calculated based  on these values are 0.83 and
0.64, respectively, which are greater than the. LOG for presumption of high risk, 0.5.
Aquatic residues measured in the Matagorda study were less and do not indicate a high
risk to acute aquatic invertebrates.  These results indicate that the risk to freshwater
invertebrates posed by use of thiobencarb on rice in the southeastern regions range from
low to high, depending;_on the^wea&er^andloc^ conditions.^ High risk probably is limited
to aqualc habitats near the"discharge of taiiwater and during times when heavy rainfall
occurs soon after thiobencarb is applied.

       Measured aquatic residues from monitoring in California were no greater than 37
ppb.  An acute risk quotient based on this value is 0.37, which is less than the LOG of
0.5. Therefore, use of thiobencarb on rice in California is expected to pose minimal acute
nsjcto ||esJMa|$r inyerjg^                              for freshwater invertebrates,
in general, there is still a concern for threatened and endangered species.

       Based  on  a chronic toxicity study  with the Daphnia magna, thiobencarb is
predicted to cause chronic effects in freshwater invertebrates  when concentrations remain
near or  above  1 to 2 ppb for an extended period of days.   Aquatic residues measured
during the two biological field studies indicate that this condition will commonly occur in
areasWhere.iMobencarbjs^applied.  For example, of the 24 samples analyzed from Area
II of Halls Bayou between 24 March and 30  April, all but two  had thiobencarb
concentrations  greater than 2 ppb.  Even in the Matagorda study, where residues were
generally lower, measured residues equal or exceeded 1 ppb for 9 consecutive days in
1983 and 7 consecutive days in 1984.  During periods of peak exposure,  residues were 12
to 406 times greater than the MATC for Daphnia magna. These findings indicate that use
of thiobencarb on riceJin the southeast regions poses a definite  high risk of causing chronic
effects to  freshwater invertebrates.  Adverse  effects  to freshwater invertebrates are
expected to occur frequently.

       Use of thiobencarb on water-seeded rice in California is predicted to pose less of
a chronic risk to aquatic invertebrates than in the southern rice-growing regions.  Water
sampling from the Sacramento River and  San Juaquin  River show  that thiobencarb
concentrations never exceeded 1 ppb, the NOEC for Daphnia magna.   The majority of
readings were  less than 0.05 ppb.  In contrast,  concentrations in smaller  waterways
c
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             For  uses other than rice, acute and chronic risk quotients  for  freshwater
      invertebrates are given below.

Table 36.  Risk Quotients (RQs) for Freshwater Invertebrates Based on a Daphnid ECSO and a
Daphnid MATC
Use Site
Celery (FL)
Lettuce and endive (FL)
LC50
(ppb}
100
100
MAJC
(Ppb)
1.7
1.7
EEC Initial
(ppb)
186
140
EEC 21-Day
(ppb)
173
130 .
Acute RQ
(EEC/LCyo)
1.86
1.40
Chronic RQ
I CEEC/MATC)
101.76
76.47
             Acute and chronic risk quotients, exceed the LOG for high risk.  These results
      indicate that use of thiobencarb on vegetables in Florida, at the maximum label rate, poses
      a risk to freshwater invertebrates due to both acute and chronic effects.  Because the
      chronic risk quotients are extremely high, chronic effects on invertebrates are expected to
      be severe.              ,                          .

                                  (e)    Estuarine and Marine Animals

             Based on aquatic residues  measured in the biological field study at Halls Bayou,
      use of thiobencarb on dry-seeded  rice can result in concentrations of thiobencarb of 690
      ppb.  This represents a near worst-case scenario when heavy rainfall occurs immediately
      after thiobencarb is applied.  Concentrations this great would exceed the acute LC50 for
      the mysid (150 ppb) and  eastern oyster (320 ppb), and would be close to the LC50 for the
      sheepshead minnow (660 ppb). They would also approach or exceed the LC50 values that
      Borthwick et al. (1985) reported for the Atlantic silverside, tidewater  silverside, and
      California  grunion.  These findings  indicate that the use of thiobencarb  on rice in the
      southeast regions poses a high acute risk to estuarine fish, crustaceans (including shrimp),
      and mollusks  at times of  high exposure resulting from heavy rainfall occurring soon after
      .application.

             Other than the one measurement of 690  ppb, however,  concentrations in Halls
      Bayou were  not greater than  83 ppb.  Furthermore,  two years of sampling in the
      biological field study near Matagorda found that residues reached a maximum of 21 ppb.
      Using the exposure value of 83 ppb, the risk quotients for fish, mollusks, and shrimp are
      0.13,  0.26, and 0.55, respectively.   This indicates that,  under conditions other than those
      described above, the acute risk is minimal to fish and mollusks. The RQ still exceeds the
      LOG, however, for high risk for shrimp and other crustaceans.

             The measured concentration of 690 ppb, representing a high exposure associated
      with heavy rainfall, indicates a high chronic risk to fish and crustaceans.  In addition, a
      high chronic  risk to crustaceans exists even for exposures measured not associated with
      heavy rainfall.  The MATC from chronic, studies with,crustaceans found MATCs that
      ranged from 4.5 to 35 ppb.  Water concentrations measured near Matagorda exceed the
      lower bound of this range,' and those'measured at Halls Bayou exceed even the upper
                                          79

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                    bound of this range.  The lower end of the .range was sometimes exceeded for several
                    consecutive, days in both of the biological field studies (for example, 8 days in Area II of
                   :,H,al|s,, ;B;aypu,	4 davsn_ at	Station^ 1	on the_ canal	in	the	Matagorda study in 1984).  It is
                    therefore clear that use of thiobencarb on rice in the southeastern regions poses a high
                    chronic risk to shrimp and other aquatic crustaceans.

                           Chronic risk to fish is less certain.  A test concentration of150 ppb caused reduced
                    growth in the sheepshead minnow.  High exposures of thiobencarb, such as the 690 ppb
                    measured at  Halls Bayou, will exceed this level,  but probably for only short periods of
                    time,  Monitoring data show that peaks in aquatic residues usually last for only a day or
                    two (Fig. 1).  Under typical conditions, maximum exposures would be considerably less
                    than l5Gppb. For water^seeded.rice, thipbencarb concentrations are expected to exceed
                    150 ppb in the field, but it is not known if they would exceed 150 ppb in off-site aquatic
                    habitats 5  The available data  thus indicate that thiobencarb has the potential to cause
                    chronic effects on estuarine fish in the southern growing region.  However, the level of
                    this risk is highly uncertain because a chronic NOEC has not been determined.

                           In California, the only estuarine habitats that are likely to be exposed to  significant
                    residues of  thiobencarb  are  bays  near San Francisco  that receive water from  the
                    Sacramento River and San Juaquin River.  All of the rice-growing region in California is
                    drained by these  two rivers. Stringent regulation requiring the retainment of floodwater
                    from rice fields have greatly reduced concentrations  of thiobencarb  occurring in  the
                    Sacramento-San Joaquin Delta since 1985 (Bailey, 1993).  Monitoring data show that
                    between 1991 and 1993 thiobencarb concentrations in these rivers are always less than 1
                    ppb, and are usually below 0.1 ppb (MacCoy et al., 1995).  This level of exposure would
                    not be  expected to cause any significant acute or chronic effects to any estuarine fish or
                    invertebrate.  Because concentrations in the bays would be no greater than concentrations
                    in the rivers that feed them, the risk assessment indicates that use of thiobencarb on rice
                    in  California poses minimal  acute and chronic  risk  to  marine/estuarine  fish and
                    invertebrates  (including shrimp and mollusks).  For uses  other than  rice,  the acute and
                    chronic risk quotients for three estuarine and  marine organisms are given below.
             Table 37.
             MATC
Risk Quotients (RQs) for Freshwater Fish Based on a Daphnid ECSO and a Daphnid
Use Site
I
Celery
(PL)
Lettuce and
endive
ป . .:;,
Test organism
Sheepshead minnow
Eastern oyster
Mysid
Sheepshead minnow
Eastern oyster
Mysid
LC5&
(Ppb)
660
320
150
660
320
150
MAtC
(ppb)
<150
ND
4.5 - 35
<150
ND
4.5 - 35
EEC Initial
(ppb)
186
186
186
140
140
140
21 -or 56-Day
EEG (ppb)
157
	 113 . ,
173
118
130
130
Acute RQ
(EEC/LCJO)
0.28
0.58
1.24
0.21
0.44
0.93
Chronic RQ
(EEC/MATC)
>1.04
—
4.9-38
>0.79
—
3.7-29
             fdfish.
                      EEC is used for assessing risk to aquatic invertebrates whereas the 56-day EEC is used for assessing risk
                                                        80
iiiiiiiiijiiiiiiiii ..... iiiiiiiiiiiiiiit/jijii:.:!.,
                  i, ....... iBiiiij'jiii: iiiiiii ....... is
                                                                       ,:	iiJiM^^^        	flii'.iiH	;„

-------
       These results indicate that use of thiobencarb on vegetables in Florida, at the
maximum label rate, poses a high acute risk to marine/estuarine oysters, shrimp, and other
aquatic invertebrates.  The RQ for chronic effects to shrimp and other marine/estuarine
invertebrates is imprecise because only supplemental data are available.  Nevertheless,
based on the range of findings from the four available supplemental studies, it is clear that
the RQ for these organisms is well above the LOG of 1, signifying a high chronic risk.

       The acute RQ for marine estuarine fish in Florida is less than the LOG of 0.5,
indicating the acute risk is not high, but it is greater than the LOG of 0.1., indicating that
restricted use may be applied.   This risk quotient also indicated that threatened and
endangered species of fish may be adversely affected.

       Definitive chronic RQs could not be determined for marine/estuarine fish because
the only available chronic fish  study failed to determine an NOEC.   However, since,
adverse effects were observed at a test concentration of 150 ppb, it is certain that both the
NOEC and MATC would have been less than this value if lower concentrations .were
tested. The chronic fish RQ may therefore be expressed as a "greater than" value.  At the
rate of 8 Ib/A, the maximum use rate for celery, the RQ is greater than 1.0, signifying a
high chronic risk.  At the rate of 6 Ib/A, the maximum use rate for lettuce and endive, the
RQ is greater than 0.79. Since this value is not much less than the LOG of 1, use at this
rate may also pose a high chronic risk to fish.  These findings thus indicate that the use
of thiobencarb on vegetables in Florida may pose a high chronic risk  to fish.   An
additional fish life-cycle study (GDLN 72-5) is needed to confirm this risk.

                     (4)    Exposure and Risk to Nontarget Plants

                           (a)    Terrestrial and Semi-aquatic

       The Agency does separate risk assessments for two categories of nontarget plants,
terrestrial and semi-aquatic.  Non-target terrestrial plants inhabit non-aquatic areas which
are generally well drained. Non-target semi-aquatic plants inhabit low-lying areas that are
usually wet, although they may be  dry during certain times of the year.  Both the
terrestrial  and semi-aquatic plants  are exposed to pesticides from runoff, drift,  and
volatilization. They differ, however, in that terrestrial plants are assumed to be subjected
to sheet runoff, whereas semi-aquatic plants are assumed to be subjected to channelized
runoff.                                                 ..-••••

       The Agency assumes .that runoff will expose nontarget plants to a fixed percentage
of the application rate. This percentage is estimated based on the water solubility of the
active ingredient:
       Water Solubility
       < lOppm
       10 - 100 ppm
% Runoff Assumed
         1%
         2%
                                    81

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         > 100 ppm
5%
         Since the water solubility of thiobencarb at 20ฐCis 27.5 ppm, the percent runoff
 is assumed to be 2%.   For non-target terrestrial plants, The Agency assumes a scenario
 in which plants are exposed from sheet runoff.  A treated site of 1 acre is assumed to drain
 into an adjacent area of 1 acre where terrestrial plants may be impacted.  In the scenario
 used  for non-target semi-aquatic plants, exposure from  runoff is assumed to be from
 channelized runoff.  A tteated site of 10 acres is  assumed to drain into a distant low-lying
 area of 1 acre where semi-aquatic plants may be impacted.

         Exposure from spray drift  was  also  assumed to  be a fixed percentage of the
 appEcafion rate.  Spray drift exposure is assumed to be 1 %  and 5 % of the application rate
 for ground and aerial applications,  respectively.
 Formulae for Calculating EECs

 Terrestrial plants inhabiting areas adjacent to treatment sites

 Unincorporated ground application:
        gjinoff Loading = maximum application rate (Ibs ai/acre) x runoff value
        Drift Loading = maximum application rate x 6.01
        Total Loading = runoff (Ib ai/acre) + drift (Ib ai/acre)

 Aerial applications:
        Runoff Loading = maximum application rate (Ibs ai/acre) x 0.6 (assumed application efficiency)
 x runoff value
        Drift = maximum application rate (Ibs ai/acre) x 0.05
        Total Loading = runoff (Ib ai/acre) + drift (Ib ai/acre)

 Semi-aquatic plants inhabiting wet low-lying areas
                                                                        i
 Unincorporated ground application:
        Runoff Loading = maximum application rate (Ibs ai/acre) x runoff value x 10 acres
        Drift Loading = maximum application rate x 0.01
        Total Loading = runoff (Ib ai/acre) 4- drift (Ib ai/acre)
:, ' „  ' "    " "       I    I I   I    I      II             I      I        ' • .                   '',..';•
 Aerial applications:
        Runoff = maximum application rate (Ibs ai/acre) x 0.6 (60% application efficiency assumed) x
                 runoff value x 10 acres
        Drift = maximum application rate (Ibs ai/acre) x 0.05
        Total Loading = runoff (Ib ai/acre) + drift (Ib ai/acre)


        Use of thiobencarb on rice is  not  expected to result in significant exposure to
 nontarget terrestrial and semiaquatic plants from runoff.  Rice fields are always bordered
 by a dike or temporary berm which would  prevent runoff from leaving the field. These
 structures do have a gate or opening for  the release of water from the field, but this-water
 is normally channeled into a stream or river.  Outflow from rice fields will therefore not
                                        82

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       normally enter dry-land and wetland habitats where terrestrial and semiaquatic plants
       occur, respectively.
                                                     f   .      i
              Nontarget plants also may be exposed from spray drift.  The Agency calculated
       risk quotients by dividing the EECs by the vegetative vigor EC25 for the most sensitive of
       the test species. Spray drift, and thus exposure to nontarget plants, will be negligible
       when thiobencarb is applied  as a granule.  The, Agency assumes that spray drift from
       liquid (EC) formulations of thiobencarb results in EECs that are 5%  and 1% of the
       application rate for aerial and ground applications, respectively!  When applied at the
       maximum label rate of 4 Ib/A, the EECs  for nontarget plants are thus 0.2 Ib/A for aerial
       applications and 0.04 Ib/A for ground applications.  The EC^ for vegetative vigor of the
 .     . most sensitive test species, ryegrass, is 0.073 Ib/A. The risk quotients are 2.7 for drift
       from  aerial applications and 0.55 for drift from ground applications.   These results
       indicate that spray drift from aerial liquid application of thiobencarb to rice poses a high
       riskto nontarget plants. Risk to nontarget plants is minimal from all ground applications
       and aerial applications of granules to rice.

              For uses other than rice, exposure to nontarget terrestrial and semiaquatic plants
       has a runoff component as well as a spray drift component. Only ground applications are
       permitted for these uses.  Estimated environmental concentrations for terrestrial and semi-
       aquatic plants are given below.

Table 38. Estimated Environmental Concentrations (EECs) For Terrestrial and Semi-Aquatic
Plants
Use Site '
Celery (FL)
Lettuce and
endive (FL)
UiSe Rate
ObaS/A)
8
6
Riiaoff
Value
0.02
0.02
Runoff Loading
Sheet Runoff
(Ibai/A)
0.16
0.12
Channelised
Runoff (Jbai/A)
1.60
1.20
Spray Drift
Loading

-------
lillFininil	in	ill!!'llimi	l.j	•MUW!TSIWS
-------
      from the Sacramento River and San Juaquin River show that thiobencarb concentrations
      never exceeded 1 ppb.  Thiobencarb concentrations in these rivers are not expected to
   •   approach levels toxic to algae.  In contrast, concentrations measured in smaller waterways
      that feed into the Sacramento River occasionally approached or exceeded the algae EEC
      of 17 ppb (Table B). These results indicate that the use of thiobencarb in California poses
      some risk of affecting algae in the smaller waterways, but minimal risk to algae in the
      larger.rivers.                        .                 -  • .

             The EC50 for duckweed is 770 ppb. This indicates that the toxicity of thiobencarb
      to vascular  aquatic plants is low relative to the aquatic EECs for rice.   However, the
      Agency believes that the phytotoxic'ity test with duckweed poorly represents the toxicity
      of thiobencarb to many aquatic vascular plants. The seedling emergence, studies indicate
      that grasses are highly sensitive to thiobencarb.  Duckweed is not a good surrogate for
      aquatic grasses and  sedges.   Duckweed is a dicot with primitive vascular structure,
      whereas aquatic grasses  and sedges are monocots with advanced vascular structure.
      Furthermore,  the primary phytotoxic effect of thiobencarb is inhibiting shoot growth of
      immature plants.  The aquatic plant test with duckweed does not address toxicity through
      this mode of action. The Agency was unable to perform a reliable risk assessment for the
      effects on emerging vascular aquatic plants.  However, since thiobencarb  is used to control
      the growth of aquatic weeds in rice fields, the Agency assumes that thiobencarb residues
      discharged into aquatic habitats poses  a risk of. killing emerging seedlings of vascular
      aquatic plants that are sensitive to thiobencarb. The risk appears to be greatest to aquatic
      grasses.   Risk to mature aquatic vascular plants is predicted to be minimal.   '

             For other uses of thiobencarb, risk quotients for-aquatic plants  are given below.
Table 40.  Risk Quotients (RQs) for Aquatic Plants Based on a the EGSO of Green Algae
(Selenastrum capricornutum)
Use Sit&
Celery (FL)
Lettuce and endive (FL)
Use Kate (LB/A)
f8
6
Test Species
Green algae
Green algae
; BCift(ppb)
. • 17
17
EEC (ppb)
186
140
RQ (EEC/EC50)
10.9
8.2
             These results indicate that use of thiobencarb  on vegetables  in Florida at the
      maximum label rate poses a high risk to nonvascular aquatic plants.  As with use on rice,
      the Agency assumes that thiobencarb residues that may reach aquatic habitats by drift and
      runoff pose a risk of killing emerging seedlings of vascular, aquatic plants that are sensitive
      to, thiobencarb.

                           (5)     Endangered Species

             The above risk assessment indicates that use of thiobencarb on rice poses a risk to-
      threatened  and  endangered  species  (TES)  of birds, mammals, fish,  and aquatic
      invertebrates (including crustaceans and mollusks).  The greatest risk is from chronic
      effects, although some risk of acute effects exists for all of these animals except birds.
                                           85

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 Use of thiobencarb on rice also poses a risk to T & E Species of plants.  All types of
 applications, may  harm  aquatic  plants, whereas only  aerial applications  of liquid
 formulations are expected to harm terrestrial and semiaquatic plants when thiobencarb is
 used according to label directions.

       Use of thiobencarb on lettuce, endive, and celery in Florida may harm all types of
 threaten^(ipriendanjer^ispecies (birds,/mammals, fish, aquatic  invertebrates, and all
 types 6f plants). 'in'IPSf.'the'USFWiS declared jeopardy from use  on Florida vegetables
 to the Snail Kite, but not to the Wood Stork or Bald Eagle; the jeopardy opinion for the
 Snail Kite was reversed to non-jeopardy in 1987 after additional data were available.

       In 1988, the USFWS determined that use of thiobencarb on rice in Arkansas may
 jeopardize the fat pocket pearly mussel (Potamilus capax}.  The current  label for Bolero
 SEC contains use prohibitions to protect this species.  These use prohibitions apply to the
 Arkansas counties of Mississippi, Poinsett,  Cross, St.  Francis and Lee where the Fat
 Pocketbook Mussel is known to occur. Comparable warnings would be appropriate where
 Use on Hce can expose other threatened 'and endangered'mussels.  In 1987, the USFWS
 determined that, because thiobencarb is not as toxic to the apple snail as to other aquatic
 invertebrates, it is not expected to jeopardize the Everglades kite.

       The Endangered Species Protection Program currently includes use limitations to
 protect mussels associated with certain rice areas. In some cases, (e.g., Arkansas), label
 requirements exist; in other areas, voluntary use limitations are being expressed in county
 bulletins being distributed to pesticide users until the program becomes final.  California
 and Florida have state-initiated plans that would be expected to protect any listed species
 in those states.  Additional use Imitations for other species and areas are expected, but
 these have not yet been defined and may be formulation specific.  Some use limitations
 could be 2evelbped by "lie Agency, but additional consultations with the USFWS may be
 necessary.  Registrants will be informed if any label changes  are necessary.
       4.     Risk Characterization
              a.
Extent of Use
       The Biological and Economical Assessment Division estimates that approximately
 1,190,000 Ib of thiobencarb active ingredient are applied on rice annually. Use on rice
 is divided into three general areas: the Gulf Coast (Texas and Louisiana), the Mississippi
 River Valley (Arkansas, Mississippi, Louisiana, and Missouri) and the Sacramento and
 San Joaquin River  Valleys in California.  A small amount of rice is also grown in Palm
 Beach and Hendry  Counties, Florida.

       A much smaller use of thiobencarb is on celery, lettuce, and endive in Florida.
 The estimatedannual use of thiobencaib on these crops is approximately 30,000 Ib ai. The
 majority of these crops are grown in Palm Beach County.

••- •'•  •  -'       ''•••""<•:'"''"  ' ';.-.^"i-	86    ;;''""":"'   '::''  ''':

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       b.     Summary of Risk Assessment
                                   ,        i        _              '••'.'..
       Thiobencarb poses a risk not only to nontarget plants (as do most herbicides) but
 also potentially significant risk to many aquatic habitats and terrestrial wildlife.  The
 ecological risks of the various uses are summarized below.

 •     Use of liquid formulations pose some acute risk to mammals.  The acute risk to
       birds is minimal.

 •     Use of liquid formulations pose a high chronic risk to birds and mammals.  The
       chronic risk from granular formulations could not be assessed.

 •     Use of tbiobencarb on rice in the southeast US poses a high risk of chronic effects
       to freshwater and estuarine aquatic invertebrates, including shrimp and mollusks.
       There  is also likely a high risk of chronic effects to fish, but additional data are
       needed to confirm this. This use of thiobencarb also poses a high risk of acute
       effects to fish and aquatic invertebrates in certain high-exposure situations.

 •     Use of thiobencarb on rice in California poses a risk of causing chronic effects to
       aquatic organisms in the smaller drains and waterways, but not in the larger rivers.
       Its use poses  minimal risk  of acute effects to fish  and aquatic invertebrates
       Minimal risk of both acute and chronic effects is expected for all estuarine
       organisms in California.

 •     Spray drift from aerial application of liquid thiobencarb on rice poses a high risk
       to nontarget terrestrial and semiaquatic plants.   Drift of granular thiobencarb and
    -   spraying of liquid thiobencarb applied with ground equipment  pose minimal risk
       to these plants.

 •      All uses of thiobencarb on rice may pose a risk of killing emerging seedlings of
       aquatic plants, especially aquatic grasses. Use of thiobencarb on rice may pose a
       risk to  aquatic algae in the southeast US and in smaller drains and waterways in
       California.

 •      Use of thiobencarb on celery, lettuce, and endive in Florida poses a high risk of
       causing  chronic effects  to fish,  freshwater  invertebrates,  and  estuarine
       invertebrates, including shrimp.   Additionally,  this use poses a high  risk of
       causing acute effects to freshwater and estuarine invertebrates, including  oysters
       and shrimp.

•      Use of thiobencarb on celery, lettuce, and endive in Florida poses a high risk to
       terrestrial plants, semiaquatic plants, and algae.  It may also pose a  risk to
       emerging seedlings of vascular aquatic plants.       ,         .   .

                                   87                                "        •

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                   c.     Impacts to Water Resources

                          (1)    Ground Water
             Although thiobencarb does exceed several of the criteria for the proposed ground
      water restricted use rule, the Agency does not consider thiobencarb to be a candidate for
      restrictelluse due to ground water concerns.  The Agency does not consider use of
      th|obencarb to be a concern in ground water, nor a human health concern from residues
      in drinldng wafer that are derived from ground water.

                           (2)    Surface Water

             Detectionsi of^ thiobencarb in water samples were relatively rare in the STORET
      database of the Office of Water, EPA.  Thirty-nine positive detections were reported for
      3,130 samples, with a maximum concentration of 0.24 mg/L and a mean concentration of
      0.10 mg/L. Surface water concentrations measured in biological field studies were several
      orders of magnitude g^j^f than this. The field study measurements were taken in surface
      water that was"11]uaame(!kMy~adja<^i^'to rice fields and were taken soon after thiobencarb
      was applied.  This suggests that high levels of contamination of thiobencarb in surface
      water is limited to local areas and for brief time periods.

             Ulilike the fltered sample 'results" reportedin STORET, results reported in the
      biological field studies may have been from uriiiltered samples.  As thiobencarb tends to
      partition more into sediment than water, the presence of suspended sediment in the water
      samples may have contributed to higher concentrations being reported in the field studies
      than in the STORET database.

              Aquatic EEC mDeling for lettuce, endive and celery uses estimated relatively high
      levels of contamination in surface water.  The range of aquatic EECs was 140 mg/L for
      the 6 Ib a.i. application rate and 180 mg/L for the 8 Ib a.i. application rate. Thiobencarb
      is expected to 'dissipate in pb"ii3!"water at an approximate rate of 0.4-0.6 mg/L/day.

                    d.     Environmental Fate and Risk Characterization

                           (1)     Rice in Southeastern United States
      iii"1 ' •      III               *               II                   i1

                                  (a)   Terrestrial Ecosystems

              The main risk from tMobencarb to terrestrial vertebrates (birds,  mammals, reptiles,
      and terrestrial stages of ampnlbians7) is from reproductive and chronic pathological effects.
  1 No data were available for reptiles or amphibians; therefore, risk to these organisms is inferred from the assessment
of risk to birds and mammals.

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The risk assessment for mammals determined that there is also a risk of acute mortality
from all uses of thiobencarb.  However, maximum acute RQs for mammals range from
less than approximately 0.5 for use on rice to slightly greater than 1 for use on celery.
These RQs are much smaller than those for risk of chronic effects to these organisms.  In
general, the impact to terrestrial ecosystems from acute effects is expected to be of little
significance, whereas the impact from chronic effects is considered to be of greater
significance.

       Use of thiobencarb in the southeast is expected to harm terrestrial vertebrates by
causing chronic physiological effects and, in the case of birds, impairing reproduction.
The levels that cause chronically toxic effects in both birds and mammals were quite low
for a pesticide that is used at high rates (4  to 8 Ib ai/A).  Furthermore, thiobencarb does
not degrade rapidly in the terrestrial environment. The combination of these factors result
in EECs remaining at levels that may cause chronic effects for several weeks (Tables B and
C).  While reproductive and other sublethal effects may result from short-term exposures
(one week or less), there is higher certainty that these longer-term exposures will cause
these effects.  Also, the longer exposure in the environment allows greater opportunity for
more organisms to be exposed.

       The Biological and Economic Analysis  Division (BEAD) estimates the typical use
rate of thiobencarb on rice is 3 Ib ai/A. Compared to the maximum label rate, use at this
rate would decrease exposure levels, and thus the risk quotients, by 25%.  Chronic risk
quotients for birds and mammals would still be great enough to indicate a high chronic
risk.      .

       The timing of application of thiobencarb on rice in the southeast region is from
March through June. This corresponds with the breeding season of birds and mammals.
Avian reproduction studies have shown that thiobencarb may decrease the number of eggs
laid or chicks hatched  at  dietary concentrations that are  similar to environmental
concentrations expected in some wildlife food. It thus appears likely reproduction could
be impaired in birds that feed in rice fields that have been sprayed with thiobencarb.

       For  the reasons" stated  above,  the  chronic risk to terrestrial organisms  is
characterized as "high".. It is not characterized as "very high" because thiobencarb is only
moderately persistent in terrestrial environments and its bioaccumulation is only moderate.
Also, except for flooded fields in Louisiana, the use  of rice fields by wildlife is not
expected to be great during the spring in the Southeast (Gusey and Maturgo, 1973):

       Another threat to terrestrial ecosystems in the southeast region is the potential harm
to nontarget plants. In this area, thiobencarb is usually  applied in a liquid EC formulation
which may be sprayed from either aerial or ground equipment.  Spray drift from aerial
applications are predicted to pose a high risk to  some nontarget terrestrial and semiaquatic
plants. This may degrade the  quality of habitat close to rice fields.  This risk may be
minimized by applying thiobencarb with ground equipment.     "

            .                     >   89

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      ^

*!""'
                               (b)    Aquatic Ecosystems
                                                                                      1
   Acute Risk to Pish and Invertebrates

         Rice is grown along the Gulf coasts of Texas, Mississippi, and Alabama, often in
   areas extending many miles inland, as well as in Palm Beach  and Hendry counties in
   Florida. Data from the U.S. NationalCHmatolpgical Data Center (USNCDC) indicates
   that rainfall in this area is typically around 60 inches per year and that storms of three
   inches or more occur about once per month.  The rice farmer cannot always retain this
 .vqlumijjf,,Fa|eฃos;the paddy8.  Therefore farmers might apply thiobencarb one day and
   be forced to releasejtthrough the drain gate the next due to unforeseen heavy rains.

          Discharges from rice fields following heavy rain events are expected to result in
   several hundred parts per billion of thiobencarb for short durations in some small estuaries
   and streams. Mpnitgring data is available (Section C.l.b.7) showing that these discharges
   can raise water concentrations in.biologically productive estuaries to levels exceeding the
<*iM^.'sl2r ^ and a(luatic invertebrates.  In general, the Agency would expect fish Mils
   arid invertebrate Mils due to acute exposure to be localized and to be confined to bodies
   of water near rice fields that have little flow or tidal flushing.9  During the Matagorda
  study,  there was a fish Mil involving menhaden in such an area.  The flesh residues of
  thiobeuearb in these fish indicate that thiobencarb may have caused the Mil. However,
  since other pesticides were being used in the area, it is not certain that thiobencarb was the
  ?ฐle cauงe, The Agency Miows of no other fish Mils that have been linked to thiobencarb.
  There is no evidence to suggest that acute risk to  fish is a major, widespread problem.
 ;<:'•• Mi	 SOU ,!'  '  •''.	:'	M.'1,,:(.)".!!! *. I]1'1* I:'1'. IK	SiJirSi1""-1!,!1"!	;!'ซ' i:,:. , u", *'Jt,l'iซ: iSClDL,1!1! MK.**ri<'.M ••'..>'•  ','1  ••'.<• •  •             : '
         The acute toxicity of thiobencarb to aquatic invertebrates is about the same as that
  for fish.  Some .acute mortality of crustaceans™andoyster larvae may occur in localized
.^areas neari rice'.fields where high levels of contamination may occur after heavy rainfall.
  Tn? to:^ityio ฐ*er invertebratessuch as corals and jellyfishes (phylum:  coelenterata),
  rotifers (phylum: rotifera; protozoan like organisms which are abundant in freshwater) has
  not been determined.
      The ric* plant is particularly sensitive to flooding when, the stem is long and thin soon after germination. At that
 time the farmer would try to maintain the water level between. 4 to 8 inches.  (Personal comm., Dr. Steve Linscombe •
 LSTJ).                                                                              •   :'•     '
                                                                                        In the
 Grulf Coast, % difference between high iand low tides is only about two feet, which is quite small compared to California
 or the Northeastern ..... Coast.  ,,    ............................ ....... i ........................ _ ............           •     '.,-'..


     Subphylum Crustacea, which is comprised of such common animals as shrimps, amphipods (scuds), and copepods,
 is the most numerous of the marine planktonic animals.

      '•'   ..... :"::"  "'  • "    :''' '•'•' :  '•'  :       90 ...........

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              Rice is also grown in the Mississippi River Valley. Rainfall data from Little Rock,  '
       Arkansas, which is near that state's rice growing area, indicate that the rain may also be
       heavy in this area; however, the rainfall patterns are somewhat more predictable than in
       the Gulf area.  Because  unpredictable spring thunderstorms are less numerous in this
       region than on the Gulf Coast (Dr. Robert Rohli, personal communications, LSU), acute
       risk to aquatic life resulting from heavy rainfall events would be less here than in the Gulf
       Coast area.

       Chronic Risk to Fish and Invertebrates             •,

              Exposure to thiobencarb poses extremely high chronic risk to aquatic organisms,
       especially for estuarine  and freshwater crustaceans in the Gulf  Coast region.   As
       mentioned above, heavy rains may result in thiobencarb concentrations in aquatic habitats
       which greatly exceed chronic toxicity levels of Crustacea. Monitoring data from Halls
       Bayou found concentrations in water at the point of inflow from ricefield drainage of
       several hundred ppb.  This far exceeds the EC^ for chronic effects11 of around 10 ppb for
       the mysid, and the chronic MATC for the opossum shrimp of 4.5 ppb. High levels are
       expected to persist long  enough in some areas to cause chronic effects.   Clearly, the
       chronic risk to shrimp and other crustaceans is very high and there is potential for high
       impact on these populations (See  Risk to Economically Important Organisms,  below).
       There are also many other types of invertebrates that are important components of the
       estuarine ecosystem^  such as worms  (annelids), jellyfishes,  and rotifers.  The chronic
       toxicity of thiobencarb has not been tested in these species, but it quite possible that
       thiobencarb would negatively affect their reproduction as it does, to crustaceans.

              Many species  of  estuarine crustaceans  and other  invertebrates  are potentially
       vulnerable to  the chronic toxicity of thiobencarb. For example, there are over 100 species
       of shrimp alone in the Gulf. These creatures would presumably be very .vulnerable when
       they  are in.estuaries and bayous  surrounded by  rice  fields.   For reasons not fully
       explained, young shrimp mass in the estuaries and streams of the Gulf Coast in the Spring.
       This is the time when thiobencarb is being applied (see below).  Also at great risk are
       many other- crustacean species which are year-round residents in estuaries, including
       mysids, young blue crabs,  many grass shrimp (Palaemonetes sp), amphipods (scuds), and  . '
       isopods (saltwater species  related to the garden "sow bug"),  these invertebrates serve  '•
       an important ecological role because they form a fundamental component of the food web. .•" :
       They  are important fprage for young fish which use estuaries as feeding grounds. Fish '•';.-:'
       whose food web would thus be at stake include drums (e.g., redfish and croaker) and
       flounder.                                         .                              •'      '

              Thiobencarb may  also harm freshwater in the Southeast.   A study submitted to
       fulfill  a test requirement of the Health Effects Division (Guideline Number 171-4f,  MRID  .
     The EC05 for chronic effects is the concentration that, based on effects observed in laboratory tests, is predicted
to cause a 5% reduction in the reproductive parameters in mysid. This level was estimated using nonlinear regression.
                                           91

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        43404003) provides information that is useful for assessing ecological impacts to these
        habitats.  This study measured residues of thiobencarb in natural freshwater habitats
        receiving discharges from rice fields in Arkansas and Texas.  In both areas, thiobencarb
        was appile^ at ajppToHmaMy'ffie'maximum" laB'el'"rate 6'f"4"lb ai/A.  In the Arkansas,
        residue levels measured in Bayou Bartholomew remained low, less than 1 ppb. In Texas,
        however, much greater residues  were measured in West Bernard Creek.   Residues
        measured at one sample point (EB1) remained greater than 1 ppb for 30 consecutive days.
        At another sample point (EB3), residues were at or greater than 4 ppb for 18 consecutive
        days. The concentration profiles in West Bernard Creek were also characterized by peaks
        of higher residues levels that were associated a flush and a rainfall event.  During these
        peaks, concentrations of 20 to 42 ppb were measured for one to three consecutive days.
        As the reproduction of the waterflea, Daphnia magna, has been shown to be adversely
        affected by 21-day exposures  as low as  3 ppb, it is  clear that the chronic  risk to
        invertebrates in West Bernard Creek would be high.  Thus, this study corroborates our
        conclusion that the use of thiobencarb on rice in the Gulf coast area poses a high risk to
        freshwater invertebrates; andthef eby could "cause serious harm to freshwater ecosystems.

        Risk to Economically Important Organisms

               Pink shrimp (Penaeus duroaruni), brown shrimp (Penaeus aztecus), and white
        shrimp (Penaeus setiferous) make up the bulk of the commercial shrimp harvest in the
        United States.  (These and other species in the family Penaeidae are commonly referred
        to as "penaeids".)  The largest harvests are of the brown shrimp. In total,  hundreds of
        millions of tons of shrimp are taken by U.S. ships in the Gulf of Mexico.   Overall the
        shrimp industry is worth billions of dollars to the economy of this country12.
             ,<: aill j In !'„: ' ; t "?; ;•',. :ซ Hit" , fill- r'
                                 Mi !ซli ii-W'f HI ill. * I™ W-'XXl if '•> ', 'Vit'lKfSf, \ ..... ปl !:' ; i1?; ..... ISMSai '. '
               The natural history of the three commercial shrimp species puts  them into a
        position to receive maximal concentrations of thiobencarb. They breed offshore with each
        of the females shedding thousands of demersal (sinking) eggs. After hatching, the shrimp
        larvae and postlarval stages  are planktonic. The postlarvae utilize multiple stimuli,
        including diminished salinity and variations in light, to position themselves in currents so
        as to be carried into estuaries.  In the estuaries they metamorphose to become benthic
        (bottom dwelling) juvenile shrimp.  Some young shrimp are particularly at risk because
        they migrate for miles up streams that feed the estuaries, even into freshwater13. Given
        that these streams would often be surrounded by rice farms, there is a great hazard to these
        young shrimp.

              "Rice growers typically apply thiobencarb beginning in April and ending around
        June. The young sensitive stages of the commercial shrimp may be exposed to release of
,	, ป  12
      Economic information provided by Mr. Larry Simpson Director of the Gulf States Marine Fisheries Commission,
 Ocean Springs, MS.

       Information provided by Dr. T. Minello, National Marine Fisheries Service, Galveston, TX.

           	92

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thiobencarb in tailwaters, particularly around June, > when the arrival of the planktonic
larvae begins-in-the estuaries of the Gulf. The exposure of young commercial shrimp
would probably continue beyond June  as thiobencarb  is considered to be  at  least
moderately persistent in some conditions.  In habitats with high turbidity or deep water,
the lack of penetration of light could make the rate of photolysis considerably slower than
was measured in the laboratory.  In these environments, thiobencarb might remain
associated with sediments and organic matter on the bottom for several weeks.  Being that
the juvenile shrimp are benthic, they would be exposed to these contaminated sediments.

       The  case regarding chronic toxicity to penaeid shrimp  is considered strong.
However, there is some uncertainty in that the exposures observed in the field are of
shorter duration than those in chronic laboratory tests.  Even the shortest chronic marine
crustacean tests are 28 days long. Monitoring data from all site (other than the flooded
paddies, themselves) do not  show continuous aquatic residues in the range of the mysid
LCos (approximately 9 ppb) for a duration this long. On the other hand, it is impossible
to know whether the full 28 days are required for adverse effects to occur.  Therefore, it
is prudent to assume, exposure to shrimp and other crustaceans represent a high risk of
chronic effects.

Risk to Aquatic Plants

       The  risk of thiobencarb to aquatic plants is uncertain.  Although highly toxic to
nontarget plants, thiobenearb appears to act mainly through preventing seed germination
and/or early seedling growth.  The reproduction of some aquatic plants, especially, annuals
that reproduce mainly through seeds, may be at risk. This may cause some damage to
vegetation growing along the edge of waterways where thiobencarb is discharged.

       Thiobencarb is quite toxic to some algae including the very important green algae."
There is a high risk of thiobencarb causing some effects,  but our current algae toxicity
studies cannot be used  to judge whether the effects would have permanent impacts on algae
populations.

                     (2)    Rice in California

                           (a)    Terrestrial Ecosystems
                                       f" '                s

       In the California rice-growing region, thiobencarb is applied mainly in granular
form  to flooded rice  fields. This use of granular thiobencarb is not expected to pose a
significant acute risk  to birds and mammals.   Because of the relatively low toxicity to
mammals, the mass of granules required to equal an LD50 substantially reduces the
likelihood of significant'acute risk to mammals.  For example, based on the rat oral-LD50
of 1080 mg ai/kg, a  100-g mammal has a 50%  probability of being killed if it ingests
approximately 110 mg of active ingredient in one day. Since thiobencarb granules contain
10% active ingredient, this is equivalent to ingesting 1.1 g of granules in one day. This
                                    93

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       is much more than a mammal of that size would be expected to ingest incidentally while
       foraging. Furthermore, most of the granular product would land in standing water and
       thus would not be available to  mammals foraging on land. There is also some use of
       liquid thiobencarb in California for rice grown with pin-point flood culture.  The acute
       risk from this use would be similar to that for the southeastern region, that is, there would
       be an acute risk to mammals but not to birds.
                                                * 	

              All uses of thiobencarb in  California potentially pose a high  risk of  causing
       reproductive  and  chronic  physiological  effects  in  terrestrial  vertebrates.   The
       characterization of chronic risk from the use of liquid thiobencarb in California is identical
       to that described above for the Southeast.: For use of granular thiobencarb on flooded rice
       fields, a quantitative assessment of chronic risk could not be .performed; however,  the
       Agency considers that exposure  of terrestrial vertebrates to granular thiobencarb poses a
       Chronic risk.  The numerous waterfowl and wading bird species  that breed in flooded rice
       fields of California14 could be adversely affected if they  are exposed .   The active
       ingredient should rapidly dissipate from the pesticide granules once it enters a body of
       water.  Therefore, granules that fall in the flood water should not pose a hazard to
       terrestrial organisms.  However,  some granuies ^Q WJQJ fall on the ground and in
       vegetation on levees and around the edges of rice paddies.  Birds may intentionally ingest
       these granules for grit. Many birds pick up grit, which helps to grind food in the gizzard.
       Birds and.other terrestrial animalsmay also ingest granules, contaminate food items, and
       contaminated soil when foraging for food on the levees and field edges. Birds, mammals,
       reptiles, and amphibians that live in  the water would also be exposed to thiobencarb
       dissQlyeJ  in  the: watej  thrQugh dermal absorption and by drinking,  although1  the
      " concentrations in water are expected to be relatively low.
      .'ซ;<" ! .  '"' ,   111  I        I    111        I I I II   I  I        Mill      I    .1 .ป.' '  '  ' 	 ,   .  ;   '  • , '.  '
              The granular formulation of thiobencarb uses a clay carrier.  Best and Gionfriddo
       (1994) found that house sparrow  were less likely to ingest granules made of clay, corncob,
       or gypsum than those made of silica.  However, further studies under different conditions
       Showed conflicting results.  Stafford et al. (199S) found that the  hazard to house sparrows
       was no greater for silica granules than for clay granules.  Compared to clay granules,
       granules made  from corncobs  were found  to pose less risk  to house sparrows when
       abundant food was provided (Stafford et. al, 1996), but greater risk when  food was
       restricted (Stafford and Best, 1997).  The relative hazard of clay, silica,  and corncob
       granules is dependent  on soil  moisture and weather conditions,  and thus cannot be
       generalized. There is not sufficient evidence to conclude that the use of clay as a granular
       carrier either increases or decreases the  hazard of granular thiobencarb to birds compared
       to if silica or corncob carriers were used.

     Walerfbwl species that breed in. the California rice-growing region include the mallard, wood duck, cinnamon teal,
northern shoveler, gadwall, redhead, ruddy duck, pied-billed grebe and eared grebe. Breeding wading birds include the
great egret, snowy egret, green-backed heron, black-crowned night heron, Virginia rail, sora rail, common moorhen,
American coot, black-necked stilt, and American avocet.

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       Avian reproductive effects observed in laboratory tests were associated with long-
term exposure to thiobencarb for approximately 20 weeks.  Exposure in the wild via
ingestion of granules likely would be for a much shorter duration.  Thiobencarb is applied
only once a year, and rainfall will cause the active ingredient to wash out of the granules
and cause the granules to become incorporated into the soil. These factors would cause
the exposure from granules to be relatively short-lived.  Some hazard would remain from
ingestion of contaminated soil and vegetation, but these routes of exposure are likely less
important than direct ingestion of granules.  It is thus uncertain if the exposure from
granular  applications of thiobencarb would be long enough or  great enough to cause
reproductive impairment in birds.                      ,       •

       The predominance of the use of the granular formulation in  California reduces the
risk of harming nontarget terrestrial and semiaquatic plants.  Granules generally will fall
rapidly from the air without drifting to off-site habitats.  Also, as discussed in the risk
assessment, the presence,of levees around rice fields precludes exposure from runoff into
terrestrial habitat. The Agency therefore assumes minimal exposure, and hence minimal
risk, to nontarget terrestrial and semiaquatic plants from the use of granular thiobencarb
in California.  Aerial application of liquid thiobencarb on rice with pin-point flooding is
predicted to pose a high risk to these plants; however, this is currently a minor type of use
in California.                       .   -

                            (b)     Aquatic Ecosystems

       The state of California has vast acreage of rice in the Sacramento River Basin and
some acreage in the San Joaquin River Basin. The State has imposed mandatory holding
periods before treated water from farms can be discharged.  Holding periods are practical
in California because rainfall is quite low and emergency discharges of flood water are
generally  unnecessary.    Monitoring  of California waterways  indicate  that water
concentrations of thiobencarb rarely reach toxic levels in agricultural drains and never
approach toxic levels in the  Sacramento and San Joaquin Rivers.  Therefore, the Agency
concludes that risk to aquatic habitats in California is limited to these agricultural drains
in areas with intensive rice production.

                     (3)    Vegetables in Florida

                            (a)     Terrestrial Ecosystems

       Thiobencarb is registered for use on celery, lettuce, and endive in Florida,  the
maximum use rate for these crops is  50-100% greater than that for rice.  The risk posed
to terrestrial and aquatic organisms is likewise  greater. Although these uses  are limited
in area, the risks they pose to local terrestrial and aquatic ecosystems are extremely high.

       Use of thiobencarb  on lettuce, celery, and endive poses a risk of causing acute
effects to mammals.' This risk is somewhat more significant for these crops than for rice.
                                     95

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11
       .The riskisii(highest for use^on celery, for which the maximum EEC on food items exceeds
        the estimated rat LCsq.  Nevertheless, since the RQs are not very large (about 1 or less),
        this risk is characterized as low to moderate.
               In contrast, the risk of reproductive and chronic effects to terrestrial vertebrates
        is very high. On lettuce, chronic RQs are as high as 14 for birds and 72 for mammals.
        RQs are even greater for celery, as high as 19 for birds and 57 for mammals. Because
        thiobencarb is moderately persistent in the terrestrial environment, the high risk from a
        single ฃpp|jcatiOE fe ppg^^gjj to 'pjifsfst foj^oae to two months (Tables E and H).  The
        long duration of this exposure increases the certainty that chronic effects will occur, and
        also provides a  greater opportunity for more organisms to be exposed.

                Birds,  mammals, and reptiles will likely be exposed as they feed in and around
        these fields,  The greatest exposure would likely be to resident ducks and  geese and
        herbivorous small mammals.  Because thiobencarb is applied during the spring, these
        animals would be exposed during the time of breeding.  Therefore, serious impairment of
        reproduction may occur.

               For the  above reasons, the Agency characterizes the chronic risk to terrestrial
        vertebrates frpm use of thiobencarb on celery, lettuce,  and endive as very high.
         se of thiobencarb on celery, lettuce, and endive is expected to result in little
exposure to nontarget plants from spray drift because aerial application is not allowed on
these crops.  Nevertheless, the risk assessment indicates that spray drift from ground
applications of thiobencarb on celery at 8 Ib ai/A poses a risk of harming the vegetative
vjgpr of nontarget plants. As the RQ was very close to 1, this risk is considered to be of
minor importance.

       The primary mode of action of thiobencarb in controlling weeds is killing weeds
before they emerge.  Not surprisingly, then, thiobencarb poses a high risk to emerging
seedlings of nontarget plants. Unlike use on rice, use of thiobencarb on vegetable crops
in Florida may contaminate soil in off-site terrestrial  habitats through runoff. Seedling
emergence" phytotbxicity studies have shown that some plants, especially grasses, are
highly sensitive to thiobencarb at the seedling  stage.  The primary endpoint affected
appears to be mortality of the plant rather than just reduction in growth.  Furthermore, the
EECs are 5 to 44 times  greater than the level predicted to kill 50% of seedlings (i.e. the
LC50) of sensitive plants.  These results indicate that thiobencarb will kill most or all of
the emerging seedlings  of sensitive plants exposed to levels equivalent to the EEC.

       The exposure to  nontarget terrestrial and semiaquatic plants is predicted to occur
more from runoff than from spray drift. The exposure from runoff may be overestimated
because thiobencarb has  a high potential to bind to soil  over time. The model used in the
risk assessment does not  take this soil binding into account.  If rainfall occurs within a day
or two after application, then exposure from runoff would be great and the high risk
                                          ' i *\I'JL\ .:.'l'.1Jii.,!M.7|1fl>t, rjii1

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 predicted by the risk quotients probably would be accurate.  If rainfall does not occur for
 "several days, however, then much of the chemical would be bound to the soil at the -site
 of application and would not be transported by runoff.  The Agency still believes that a
 high risk to these plants exists because the RQs are quite high and because heavy rain is
 frequent and unpredictable in Florida.

       The ecological impact of killing emerging seedlings of nontarget.plants is largely
•unknown.   Frequent exposure within a given area to a herbicide of this  nature'may
 potentially reduce the number of sensitive plants such as annual grasses.  This may alter
 the composition of the plant community, which may cause unpredictable effects on the
 ecosystem.

                            (b)     Aquatic Ecosystems

       Thiobencarb is used  on lettuce,  endive, celery and rice in Florida.   The total
 acreage  of  these crops in Florida is probably less  than 40,000 acres (1992 Census of
 Agriculture).  Runoff to surface water will likely cause aquatic concentrations  that greatly
 exceed the  Levels of Concern for crustaceans and perhaps fish. Given  the very small
 number  of acres on which this pesticide  is applied in Florida, risk to aquatic organisms
 should be quite localized; however, the impact to aquatic habitats within these local areas
 is likely to be severe.

       Most of the use of thiobencarb  in Florida  is concentrated in Palm Beach  and
 Hendry  Counties.  These counties lie on the northern edge of The Everglades.   Water
 draining from agricultural fields treated with,thiobencarb  may be contributing  to the
 degradation  of water quality of northern sections of this very important habitat, including
 the Loxahatchee National Wildlife Refuge in Palm Beach County.   Water" samples are
 being collected along the Hillsboro  canal in Palm Beach counties as part of the NAWQA
 Program of the USGS.  Once completed, these data should indicate the degree that
 thiobencarb residues are contaminating the Loxahatchee National Wildlife Program  and '
 the northern everglades.

                     (4)    Threatened and endangered species

       Protecting threatened and endangered species from thiobencarb will be unusually
difficult.  Adverse effects are possible  for many types of plants- and animals. Probably the
greatest  threat from thiobencarb is  to aquatic organisms in freshwater  and estuarine
habitats near areas with extensive rice production, or near large celery, lettuce  and endive
farms in Florida. Thiobencarb  can clearly cause, direct detrimental effects to aquatic
organisms,  especially small,invertebrates.  Additionally,  thiobencarb may  reduce  the
abundance of phytoplankton and zooplankton which form the base of aquatic food webs.
This may^ cause  additional indirect  effects to animals at higher trophic levels.  It is .•
therefore important that thiobencarb be prevented from contaminating occupied habitats
of threatened and endangered aquatic species.  Finally, extreme care should be taken to
                                    97

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prevent contamination of the habitat of threatened and endangered plants that occur in
areas of Florida where celery, lettuce, or endive is grown.
RISK MANAGEMENT AND
; •	,	i:'•!;,, : iiifi! .i;r: '•:, • 	*;<ซ!,	;ป' iv•!.; '-"T -a ,ii '•'.•':ปป;*.si"M"	iv-iii	

A.     Determination of Eligibility

       Section 4(g)(2)(A) of FIFRA calls for the Agency to determine, after submission
of relevant data concerning an active ingredient, whether products containing the active
ingredients are eligible  for reregisixation.  The Agency has previously identified and
required the submission of the generic (i.e. active ingredient specific) data required to
support reregistration of products containing thiobencarb active ingredients.  The Agency
has completed its review  of these generic data, and has determined that the data are
sufficient to support reregistration of all products containing thiobencarb.   Appendix B
identifies  the  generic  data requirements  that the  Agency reviewed  as part of its
determination of reregistration eligibility of thiobencarb, and lists the submitted studies
that the Agency found acceptable.
       The data identified in Appendix B were sufficient to allow the Agency to assess the
registered uses of thiobencarb and to determine that thiobencarb can be used without
resulting in" unreasonable 'adverse"effete to 'Humans'	and	the environment. The Agency _
therefore finds that at this time all products containing thiobencarb as the active ingredient
are eligible for reregistration.  The reregistration of particular products is addressed in
Section V of this document.

       The Agency made its reregistration eligibility determination based upon the target
data base required for reregistration, the current guidelines for conducting acceptable
studies to generate such data, published scientific literature; etc. and the data identified in
Appendix B. Although the Agency has found mat all uses of thiobencarb are  eligible for
reregistration, it should be understood that the Agency  may take appropriate regulatory
action, and/or requjje the submission 2^ ™ ซi!i0.B^  ^al? !2 suJ?P.ort me registration of
products containing thiobencarb, if new  information comes to the Agency's attention or
if the data requirements for registration (or the guidelines for generating such data)
change.                                                                           -
B.     Determination of Eligibility Decision

       1.     Eligibility Decision
       Based.on.the reviews of the generic data for the active ingredient in this case, the
Agency has sufficient information on the health effects of thiobencarb, but has certain
limitations on its potential for causing  adverse effects in fish  and wildlife and the
environment.  The Agency has determined that thiobencarb products, if labeled and used
                                     98

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 as specified in this Reregistration Eligibility Decision, will not pose unreasonable risks or
 adverse effects to humans or the environment.  Under the Food Quality and Protection Act
 of 1996, the Agency has determined that there is reasonable certainty that no harm will
 result to  infants and children or the general population from aggregate exposure to
 thiobencarb.  Therefore, the Agency concludes that all products containing thiobencarb
 are eligible for reregistration. - "   -
       '2.
Eligible and Ineligible Uses
       The Agency has  determined that all  uses  of thiobencarb are eligible  for
 reregistration subject to conditions imposed in this RED.

 C,    Regulatory Position

       The following is a summary of the regulatory  positions and rationales  for
 thiobencarb.  Where labeling revisions are imposed, specific language is set forth in
 Section V of this document.

       1.     Food Quality Protection Act Findings

              a.     Determination of Safety for U.S. Populations

       EPA has determined that the established tolerances for thiobencarb meet the safety
 standards under the FQPA amendments to Section  408  (b)(2)(D) for the general-
 population. In reaching this determination, EPA has considered available information on
 the aggregate exposures (both acute and chronic) from non-occupational sources, food and
 drinking water, as well as the possibility of cumulative effects from thiobencarb and other
 chemicals with a similar mechanism of toxicity.

       Since there,are no residential or lawn uses of thiobencarb, no dermal  or inhalation
 exposure is expected in and around the home. No acute toxicity endpoints of concern have
 been identified for thiobencarb.

       In assessing chronic dietary risk, EPA estimates that thiobencarb residues in food
 sources account for .<. 42.9 percent (%) of  the RfD,  and includes the highest-at-risk
 subgroup, non-nursing infants.  In drinking water thiobencarb residues account for 0.29
percent (%)  of the RfD.  Thus, the aggregate  exposures from all sources of thiobencarb •
 (in this case, only dietary and drinking water exposures are relevant) account for 43.2
percent (%) of the RfD.  Therefore, the Agency concludes that aggregate  risks for.'th'e/,:,
general population resulting from thiobencarb uses  are not of concern.       . -  •  r?  V -I'v
                                                                            " • ".;' .'"*'•
                    b.     Determination of Safety for Infants and Children

       EPA has determined that the established tolerances for thiobencarb meet the  safety
standard under the FQPA,amendment to  section 408(b)(2)(C) for infants and children.
                                    99

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,'	ill!' Illiilp	WBJ(HMMI	"111!"!	i*'i	i{ll!
           The safety determinatipn for infants and children considers the factors noted above for the
           general population, but also takes into account the possibility of increased dietary exposure
           due to the specific consumption patterns of infants and children, as well as the possibility
           of increased susceptibility to toxic effects of thiobencarb residues in  this population
           subgroup.

                 to
    ;.. '     . toxic effects from thiobencarb	residues, EPA considered the	completeness of the data base
 "":  |,;,,, • '• iij'for developmental anS'reproSuctiv'e effects, ''the""nature of "3ie effects observed, and other
  "I1;1!  ;'    Information,

                 Based on current data requirements, thiobencarb has a complete data base for
 ••  "(,;!:  : ,-> MI*	{'.'(!'	i	';-'ซ'i'Vi	LWM;1'	rt.:ซ	'S;T	< •:!•*•?ป	s-	—	u-	'	i-	r	,	  w  •  •• „     •  •
           developmental and reproductive toxicity.  In the developmental studies, effects were seen
           in the fetuses only at the same or higher dose levels than effects on the mothers. In the
           reproduction study, no effects on reproductive performance were seen.  EPA concludes
           that  it is unlikely that there is  additional risk concern for immature or developing
           organisms.  Finally, the Agency has no epidemiological information suggesting special
           sensitivity of iinfants and| children to .thiobencarb.  Therefore,  EPA finds that an additional
    :!i™  ' ,   tm'celrMnty '"factor is	no-t"warranted'fOT

                 EPA estimates that thiobencarb residues in the diet of infants and children account
           for 42.9 percent of the RfD (29.5 for children 1-5) and residues in drinking water account
           for 0.29 percent of the RfD. Thus the aggregate exposure from all sources of thiobencarb
       :..  ';.' account	for' 43.2 Jje^cent of the	RfD"'for infants and' children.  Therefore,.' the Agency
        •' '^ftclu^eTfnaTaggl^^^                                      from uses of thiobencarb'
           are not of concern.

       ','.   ._, ., ,... .. |n deciding to''continue  to "make reregistiratipn Determinations during the early
      	stages of' FQPA' implernentaHon,1	EPA"' 'r^0"gnjggs	^l" |^" "w^j • ^g necessary to  make
           decisions relating to FQP A before the  implementation process is complete.  In making
           these early, case-by-case decisions, EPA does not intend  to set broad precedents for the
           application of FQPA to its regulatory determinations.  Rather, these early decisions will
           be made on a case-by-case basis and will not bind EPA as  it  proceeds with further policy
           development and any rulemaking that may be required.

                 If EPA determines,  as a result of this later implementation process, that any of the
           determinations described in this RED are no longer appropriate, the Agency will consider
           itself free to pursue whatever action may be appropriate,  including but not limited to
           reconsideration of any  portion of this RED.

    :,ป;!.'.;  • ,f-  V.'2.    ""RiskMitigation         	          '                ;
    	'.„ I,,!  I   • s, '! i •  „:''   III mil  I '.| *. '""i,11"  |  | |  | O

                 To lessen worker risk,  and ecological and water quality risks posed by thiobencarb,
           EPA is requiring the  following  mitigation measures from registrants of thiobencarb-
           cdntaining products.
                                               100

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To protect handlers:
          For liquid formulations:  mixers and loaders must used closed systems in
          addition to wearing a chemical-resistant apron, chemical-resistant gloves,
          long-sleeve shirt, long pants, shoes, and socks.  Applicators and flaggefs
          must use enclosed cabs or cockpits and wear long-sleeve shirt, long pants,
          shoes, and socks.                              .        .
 ••-•  '   For granular formulations: loaders must wear a chemical-resistant apron,
          chemical-resistant gloves, long-sleeve shirt, long pants,  shoes, and socks.
          Applicators and flaggers must wear chemical-resistant gloves, long-sleeve
          shirt,  long pants,  shoes, and socks.

To protect-workers:

           A restricted-entry interval of 24 hours is being imposed.  Early  entry
          workers must wear coveralls, chemical-resistant gloves, shoes,  and socks.

To protect non-target organisms:

          Application restrictions are being mandated in the states of Louisiana and
          Texas. In Louisiana, thiobencarb application will not be allowed south of
          the Intracoastal Waterway.  In Texas, thiobencarb application will not be
          allowed within two miles inland from the shorelines of Galveston Bay,, and
          not within two miles of Matagorda Bay.
          Include  label  warnings  preventing  application to  rice  fields  with
          catfish/crayfish farming, and preventing application to rice fields adjacent
          to catfish or crayfish ponds.
          Where weather conditions permit, it is required that flood waters not be
          released within 14 days.
          Require that thiobencarb  not be applied within 24 hours of rainfall, or
          when heavy rain is expected to occur within 24 hours.
          Require that thiobencarb not be mixed/loaded or otherwise handled within
          100 feet of aquatic habitat.
          Continue existing label warnings addressing environmental hazards, such
          as restricting application aerially within  one mile of the St. Francis
          Floodway where the Fat  Pocketbook Pearly Mussel is known ta occur.
          Comparable warnings would be appropriate where use on rice can expose
          other threatened and endangered mussels.
          Work with the EPA to reassess in the Fall of 1998 thiobencarb use on leafy
          vegetables in Florida based  on the results  of the  currently . ongoing
          environmental monitoring study for muck soils in Florida from the U.S.
          Geological  Survey's   National  Water  Quality  Assessment  Program
          (NAWQA).
                               101

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.......... "i ....... • ..... mm ........... H1F ......... ™'f tBBi ..... ซ!M^
                                                        ...... Blli ..... !Iili'!l! ..... I ......... NnMMtiWtf| ..... lilllji

                            gl , :^i'*TiTfi;W'l!*4''t''ip^?1^;i!!.T!,11^ ^'fV'v1'/ 'H:1 '" ••' ["'iyAf'H'Vii1 ..... '**! 'fi^
                                  The Agency's concerns and risk mitigation measures are discussed in more
                           detail below.

                           3.     Tolerance Reassessment Summary

                           The tolerances for plant and animal commodities listed in 40 CFR ง180.401(a) and
                    (b) are expressed in terms of the combined residues of thiobencarb and its metabolites
                    containing the  chlorobenzyl and chlorophenyl moiety.  A summary of thiobencarb
                    tolerance reassessments is presented in Table 41.

                    a. Tolerances Listed Under 40 CFR ง180.401(3."): Sufficient data are available to ascertain
                    the adequacy of the established tolerances for the following commodities listed in 40 CFR
                    ง180.401(a):  cattle, fat; cattle, mbyp (meat byproducts); cattle, meat; eggs; goats, fat;
                    goats, mbyp; goats, meat; hogs, fat; hogs, mbyp; hogs, meat; horses, fat; horses, mbyp;
                    horses, meat; mUk; poultry, fat; poultry, mbyp; poultry, meat; rice, grain; rice, straw;
                    sheep, fat; sheep, mbyp; sheep, and meat.

                    b. Tolerances Listed Under 40 CFR ง180.40irb'):  Sufficient data are available to ascertain
                    the adequacy of the established tolerances with regional registration in accordance with 40
                    CFR ง180.l(n), for the following commodities listed in 40 CFR ง180'.401(b): celery,
                    endive (escarole), and lettuce.

              Table 41. Tolerance Reassessment Summary for Thiobencarb.
|n . . , 	 	 . / ft !'.'"i ', 'ix!' ::,r; !.".,: : :, ซ,
Commodity
Current Tolerance (ppm)
Tolerance Reassessment (ppm)
Tolerances Listed XJnder^O CIR 180.40%);
Cattle (fat, meat, mbyp)
Goat (fat, meat, mbyp)
Hog (fat, meat, mbyp)
Sheep (fat, meat, mbyp)
Poultry (fat, meat, mbyp)
Horse (fat, meat, mbyp)
Eggs
Milk
Rice, grain
Rice, straw
0.2
	 0.2
0.2
0.2
0.2
0.2
0.2
0.05
0.2
1.0
0.2
0.2
0.2
0.2
' 0.2
0.2
0.2
0.05
0.2
1.0
" ;TolerancesLisW Under 40 CER180.401(b):
Celery, Lettuce, Endive (escarole)
0.2
0.2
                            4.      Codex Harmonization
                            III  I         III    I  I I  I  I I I I            I   I            ','!' ,     '  •'  '  ;  ,'. '
                            No  maximum  residue  limits  (MRJLs) have been  established by the Codex
                     Alimentarius Commission for  thiobencarb residues in/on raw agricultural, animal, or
                                                         102

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 processed commodities. Therefore, no compatibility questions exist with respect to U.S.
 tolerances.                         '                                           '  '

        5.     Reference Dose (RfD)

        The RfD/Peer Review Committee met on February 8,  1996,  to  discuss and
 evaluate  the  existing and/or  recently submitted toxicology data in support of  the
 thiobencarb reregistration and to reassess the RfD for this chemical.

        The Committee  recommended that the existing  RfD for thiobencarb remain
 unchanged.  The RfD for this chemical was based  on the two-year rat feeding study
 (MRID# 00154506) with a NOEL of 20 ppm (1 mg/kg/day). At the next higher dose
 level of 100 ppm (5mg/kg/day), decreased body weights and increased blood urea nitrogen
 levels were observed. An uncertainty factor of 100 was applied to account for both inter-
 species extrapolation and intra-species variability.  On this basis, the RfD was calculated
 by the Committee to be 0.01 mg/kg/day.                        '  •.  •   •

        6.      Cancer Risk Assessment

        The Agency has classified thiobencarb as a Group D chemical (not classifiable as
 to human carcinogenicity).  The carcinogenic potential of thiobencarb was evaluated by
• the RfD/Peer Review Committee on February 8, 1996.  The Committee considered the
 carcinogenicity phases of the combined chronic toxicity/carcinogenicity studies in rats
 (MRID#  00154506) and the carcinogenicity study  in mice (MRID# 00086004) for
 carcinogenic classification.

       The highest dose level tested in the rat (500 ppm, or 25 mg/kg/day) was considered
 to be adequate for carcinogenicity testing based on depression of cholinesterase activity
 and reduced body weight gain. The highest dose level  tested in the mouse (1600 ppm, or
 235 mg/kg/day in males and 302, mg/kg/day in females) was considered to be adequate
 based on body weight gain depression.

       7.     Occupational Exposure

       At this time, all products containing  thiobencarb are intended primarily for
 occupational use  (i.e. mixed,  loaded, and applied  by commercial  applicators only;
 generally not available to homeowners). No registered use is likely to involve applications
 at residential sites.     .

       The Worker Protection Standard CWPS1

       EPA's Worker Protection Standard for Agricultural Pesticides (WPS) affects all
pesticide products whose labeling reasonably permits  use in the commercial  or research
production of' agricultural plants on any farm, forest, nursery, or greenhouse.  In general,
                                   103

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WPS products had to bear WPS-complying labeling when sold or distributed after April
21,  1994, The WPS  labeling requirements pertaining to personal protective equipment
(PPE), restricted-entry intervals (Rg^ ^ notification are interim. These requirements
are to be reviewed and revised, as appropriate, during reregistration and other Agency
review processes.  At this time all registered uses of thiobencarb are within the scope of
the WPS.

     •  ; ........ -: • •  ',3. .......... Handler Exposure and Risk
                '
•..v , , i i '; .ill : H; .u     ..... 'iJii- ^^                           pir • ^ , '•. '• ^ .   ,  .   ••• ' > ' • •,;. •
       For each end-use product, personal protective equipment and engineering control
 requirements for pesticide handlers are set during reregistration as follows:
f         -,••'{* - ri" ..... i = ,'!'ป ;:JJ,,i 'J'fflf'ifHiajlE.'^iMWSHf^i'BBli? IpV/t ..... iEHIi ....... 'ป'V' fi'i'
-------
baseline attire consisting of long-sleeve shirts, long pants, shoes, and socks.  In addition,
loaders must wear a chemical-resistant apron.                          .       .

       Liquid Formulations: The Agency beEeves that risks resulting from intermediate-
term exposures to persons handling liquid  formulations  are overestimated  due  to
limitations  with  the hazard  identification  and  dose-response  assessment for  the
intermediate-term endpoint, particularly in light of the absence of serious effects to these
target organs in either the subchronic neurotoxicity or rat  chronic feeding study, which
suggest the lack of a deleterious response to  thiobencarb by the kidney and/or liver.
Therefore, the Agency, drawing on its experience and expertise, has determined that risks
to handlers of liquid formulations will be adequately mitigated with the use of,engineering
controls and personal protective equipment.  Mixers/loaders will be required to use closed
systems and wear chemical-resistant gloves and aprons in addition to baseline attire.
Applicators and flaggers will be required  to use enclosed cabs or cockpits  and wear
baseline attire.                          •

              b.     Post-Application Exposure and Risk

       Restricted-entry intervals, earlv-entrv PPE. and "double" notification:

       The interim Worker Protection "Standard (WPS) restricted-entry intervals (REI's)
for agricultural  workers are based solely on the acute dermal toxicity  and skin and eye
irritation potential of the active  ingredient. In addition, the WPS  retains two types of
REI's established by the Agency before the promulgation of the WPS: (1) product-specific
REI's established on the basis of adequate data, and (2) interim REFs that are longer than
those that would be established under the WPS.

       The WPS prohibits routine entry to perform hand labor tasks during the REI and
requires PPE to be worn for other e'arly-entry tasks that require  contact with treated
surfaces.

       "Double" notification is the_statement on the labels of some WPS pesticide products
requiring  employers to notify workers about pesticide-treated areas orally as well as by
posting of the treated areas.  The interim WPS "double"  notification  requirement was
imposed if the active ingredient is classified as toxicity category I for acute dermal toxicity
or skin irritation potential.                                       '                •

       During the reregistration process, EPA establishes  REI's, early-entry PPE, and
double  notification  requirements based on, consideration  of all available relevant
information about the active  ingredient, including acute toxicity, other adverse effects,
epidemiological information, and post-application data. EPA is establishing a 24-hour REI
and the following early-entry PPE  for  all in-scope WPS uses of products containing
thiobencarb; coveralls, chemical-resistant gloves, socks, and shoes.  EPA has determined
that double notification is not required.
                                    105

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        The surrogate ppst-appHcation exposure and risk assessment indicates that risks to
 post-application (reentry) workers should be acceptable provided entry is postponed until
 at least 24 hours following application.  Since thiobencarb is applied early in the season,
 when crops and weeds are small, the agency anticipates that the dermal exposures will be
 relatively low.  The Agency also concluded that the  types of post-application  tasks,
 including scouting, thinning, or hoeing, performed at this point in the crop cycle are not
 likely to result in intermediate-term exposures. The Agency also determined that  early-
 entry personal protective equipment consisting of coveralls, chemical-resistant gloves, and
 socks plus shoes would" Be:"adequatelyprotective^ if  workers must  enter during  the
 restricted-entry interval as permitted under the Worker  Protection Standard.

<              c.     Other Labeling Requirements

        The Agency is also requiring other use and safety information to  be placed on the
 labeling  of all  end-use products  containing  thiobencarb.  For  the  specific labeling
 statements, refer to Section V of this document.

        8.     Ecological Effects Risk Management

               In general,  the risk assessment showed various  levels of concern (LOG)
 regarding chronic effects  to  fish and  freshwater invertebrates, including shrimp and
 mollusks, and high risk of causing acute effects to freshwater and estuarine invertebrates.
 The following is a regional account of thiobencarb risk mitigation measures for ecological
 effects.
       	,8!!!'!
       '511!
a.     Southeastern United States (Texas, Louisiana)
    •i . i itfi-iiiiii „; vs, i	i; • i	'*j •ซ "IF, >.	i a, it	M:: >' li W) 2:''If, :-,ฃ'!	!'!: IK f i	Is •;:.' :  '.   , v
        Study findings indicate that the use of thiobencarb on rice in the southeast regions
 poses a high acute risk to estuarine fish, crustaceans (including shrimp), and mollusks at
 times of high exposure resulting from'"heavy rainfall occurring soon after application.

        For acute effects to aquatic invertebrates, calculated risk quotients are 0.83 and
 0.64, respectively, which are greater than the LOG for presumption of high risk, 0.5.
 These results indicate that the risk to freshwater invertebrates posed by use of thiobencarb
 on rice in the southeastern regions range from low to high,  depending on the weather and
 local conditions.  High risk probably is limited to aquatic habitats near the discharge of
 tailwater and during times when heavy rainfall occurs soon after thiobencarb is applied.

        For chronic effects to aquatic invertebrates, based on a chronic toxicity study with
 the Daphnia magna, thiobencarb is predicted  to cause  chronic  effects in freshwater
 invertebrates when concentrations remain near or above 1 to 2 ppb for an extended period
 of days.  Aquatic residues measured during biological field studies  indicate that this
 condition will commonly occur in areas where thiobencarb is applied.  Findings indicate
 that use of thiobencarb on rice in the southeast regions poses a definite high risk of causing
                                     106

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 chronic effects to freshwater invertebrates.  Adverse effects to freshwater invertebrates are
 expected to occur frequently.                             •

       For effects to estuarine and marine organisms, based on aquatic residues measured
.in the biological field study at Halls Bayou, use of thiobencarb on dry-seeded rice can
 result- in concentrations of thiobencarb of 690 ppb.   This  represents  a near worst-case
 scenario when  heavy  rainfall  occurs  immediately  after  thiobencarb  is  applied.
 Concentrations this great would exceed the acute LC50 for the mysid (150 ppb) and eastern
 oyster (320 ppb), and would be close to the LC50 for the sheepshead minnow (660 ppb).
 They would also approach or exceed the LC50 values that Borthwick et al. (1985) reported
 for the Atlantic silverside, tidewater silverside, and California grunion.

       Other than the one measurement of 690 ppb,  however," concentrations  in Halls
 Bayou were  not greater than  83, ppb.   Furthermore, two  years of sampling in the
 biological field study near Matagorda found that residues reached a,maximum of 21 ppb.
 Using the exposure value of 83 ppb,  the risk quotients for fish, mollusks, and shrimp are
 0.13, 0.26, and 0.55, respectively.. This indicates that, under conditions Bother than those
 described above, the acute risk is minimal to fish-and mollusks.  The RQ still exceeds the
 LOG, however, for high risk for shrimp and  other crustaceans.

       The measured concentration of 690 ppb, representing a high exposure associated
 with heavy rainfall, indicates a high chronic risk to fish and crustaceans \  In addition, a
high chronic risk to crustaceans exists even for exposures measured not associated with
heavy rainfall.  The MATC from chronic studies with crustaceans found MATCs that
ranged from 4.5 to 35 ppb.. Water  concentrations measured near Matagorda exceed the
lower bound of this range, and those measured at Halls Bayou exceed even the upper
bound of this range.  The lower end of the range was .sometimes exceeded for several
consecutive days in both of the biological field studies (for example, 8 days in Area II of
Halls Bayou, 4 days  at Station 1 on the.canal in.the Matagorda study in-1984).  It is
evident that use of'thiobencarb on rice in the southeastern regions poses a high  chronic risk
to shrimp and other aquatic crustaceans.                                ~     '   .-,
              *                          '                         '"           '
       In summary, based on the results of these tests, the Agency has decided to prohibit
application of thiobencarb south of the Intracoastal Waterway in Louisiana; prohibit
application within two  miles of Galveston Bay, in Texas,  and within  two miles'of
Matagorda Bay, in Texas; and prohibit the release of permanent flood water within 14-
days of application of thiobencarb, when feasible (depending on weather patterns).

       Additionally, the Agency mandates that  thiobencarb is not applied within 24 hours
of rainfall, or when heavy rain is expected to occur within 24 hours. Lastly,, the Agency
requires that thiobencarb is not mixed/loaded or otherwise handled within  100 feet of an
aquatic habitat.
                                    107

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               b.
              Florida
[is!
       Study results indicate that use of thiobencarb  on vegetables in Florida, at the
maximum label rate, poses an risk to freshwater invertebrates due to both acute and
chronic effects. Because the chronic risk quotients are extremely high, chronic effects on
aquatic invertebrates  may  be high,  depending  on the  environmental  transport of
thipbencarb through the muck soils 'on" which" the active ingredient is applied. The risk to
aquatic habitat in Florida, primarily  the nothern stretches of the Everglades  and the
Loxahatchee National Wildlife Refuge, at this time is uncertain.

       Study results indicate that use of thiobencarb  on vegetables in Florida, at the
maximum label fate, may pose"a high acute risk'fo marine/estuarine oysters, shrimp, and
other  aquatic invertebrates.   The   RQ for chronic effects  to shrimp and  other
marine/estuarine invertebrates is imprecise because  only supplemental data are available.
Nevertheless, based on the range of findings from the four available supplemental  studies,
it is clear that the RQ for these organisms is well above the LOG of 1,  signifying a high
chronic risk.  The acute RQ for marine estuarine fish in Florida is less than the LOG of
0-5, indicating the acute risk is not high, but it is greater than the LOG of 0.1, indicating
that restricted use may be applied, depending on the results of ongoing environmental
monitoring studies.

       At  this time, the Agency believes that the main concern for thiobencarb use on
leafy vegetables in Florida is the fate and transport of thiobencarb to aquatic ecosystems,
again, due to the pjoximity of Mobencarb application to the northern portion of the
Everglade's and""the	LolcaTfiatoh'e^'l^ation^'^ndliTe''	Refuge.  At this time, very limited
information is available on thiobencarb concentrations in the ground and surface water
travelling to these habitats.  The National Water Quality Assessment Program (NAWQA),
currently being conducted by the U.S. Geological Survey, is collecting water samples in
southern Florida  and  analyzing  the  samples for pesticides, including thiobencarb15.
Interpretation of the NAWQA monitoring data will be delayed until the collection, quality
control, and analysis of the data have been completed in the Fall of 1998.  At that time,
the Agency will reevaluate the exposure that thiobencarb poses to aquatic habitats in
Florida, and reassess the need for any risk mitigation measures for thiobencarb use in the
state of Florida.
                             c.
                            California
               Granular thiobencarb  is used only on rice grown ir California.  , Granular
        thiobencarb is estimated to pose chronic risi:"to terrestrial species,  but the risk is uncertain.
        1 To date, testing for thiobencarb has only been completed for approximately 16 samples that were taken in
 the Fall of 1996 into the Winter of 1997.  Thiobencarb was not detected in these samples.  However, the Agency
 believes that it is premature to draw any conclusions from these data since these data do not include sampling for the
 application period for thiobencarb (April/May).
iiilr	    i,,       III  I         III      II       I               I II   |l  '   '  '   ' ,i   '     '      '

••-'   •'••  ••                                     108

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        Measured aquatic residues from monitoring in California were no greater than 37
 ppb. An acute risk quotient based on this value is 0.37, which is less than the LOG of
 0.5.  Therefore, use of thiobencarb on rice in California is expected to pose minimal acute
 risk to freshwater invertebrates.                                              "

        Use of thiobencarb on water-seeded rice in California is predicted to pose less of
 a chronic risk to aquatic invertebrates than in the southern rice-growing regions.  Water
 sampling from the  Sacramento River  and  San Joaquin River  show that thiobencarb
 concentrations never exceeded 1 ppb, the NOEC for Daphnia magna.  The majority of
 readings were less than 0.05 ppb.  In contrast, concentrations in smaller waterways
 occasionally approached or exceeded the NOEC of 1 ppb, as well as the MATC of 1.7
 ppb.

       The use of the thiobencarb granular formulation in California is regulated under
 the Basin Plan for the  Sacramento River Basin established by the California Regional
 Water Quality Control Board, Central Valley Region.  A performance goal  of 1.5 ppb is
 strictly monitored,  and growers must adhere to a program  of approved  management
 practices, including a 30-day water holding restriction.

      . The Agency proposes no risk mitigation measures for granular thiobencarb use in
 California.     •

       9.      Spray Drift Advisory

       The Agency has been working with the Spray Drift Task Force, EPA Regional
 Offices and State Lead Agencies for pesticide regulation to develop the best spray drift
 management practices. The Agency is now requiring interim measures that must be placed
 on product labels/labeling as  specified in Section V.  Once  the Agency completes its
 evaluation of the new data base submitted by the Spray Drift Task Force, a membership
 of U.S. pesticide registrants, the Agency may impose further  refinements in spray drift
 management practices to  further reduce off-target drift and risks associated with this drift.

       10.     Endangered Species Program

       The Agency  has developed a program (the  '"Endangered  Species Protection
 Program") to identify pesticides whose use may cause adverse impacts on endangered and
 threatened species, and to implement mitigation measures that  will eliminate the adverse
 impacts.  At present, the program is being implemented  on an interim basis  as described
in Federal  Register Notice  54 FR 27984-28008 (July 3,   1989),  and is providing
information to pesticide users to help them protect these species on a voluntary basis.  As
 currently planned, the final program will call'for label modifications referring to required
limitations on pesticide uses, typically as depicted in county-specific bulletins or by other
site-specific mechanisms as specified by state partners.  A final program, which may be,
altered from the interim program, will be described in a future Federal Register Notice.
                                 '  109

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       The Agency is not imposing label modifications at this time through the RED. Rather,
       any requirements for product-use  modifications will  occur in the  future under the
       Endangered Species Protection Program.
V.     ACTIONS REQUIRED OF REGISTRANTS
             This  section specifies  the  data requirements and responses necessary  for the
       reregistration of both manufacturing-use and end-use products.
      /A'.	  'Sanufacturing-tlse Products
             1.     Additional Generic Data Requirements

             The generic data base supporting the reregistration of thiobencarb for the above
       eligible  uses has been reviewed and determined to  be substantially complete.   For
       confirmatory purposes, the following information will need to be submitted:

       •	Dermal Penetra^on Study [GLN85-2J;
             The dermal absorption factor in the current risk assessment is 60.2  percent,
             observed at 10 hours!' TEfclactpr is considered by the Agency as a worst-case
             scenario since the  skin was washed approximately 1 hour prior to dosing rather
             than the recommended 24 hours (which would allow for normal replacement of
             skin oils).  The Agency views this as  an over-estimate of absorption, resulting in
             significantly lower estimates for margin  of exposure to workers, specifically
             intermediate-term exposure. A new dermal penetration study is requested for the
             granular and liquid thiobencarb formulations to  confirm that intermediate-term
             exposure to workers is acceptable.

       •     Life-Cycle Freshwater Fish Study [GLN 72-5];
             There is currently no  core data on  the chronic effects of thiobencarb on fish
             (freshwater or saltwater).  Use of thiobencarb on rice is  expected to  result in
             extensive exposure to freshwater habitats, thiobencarb degrades slowly in water,
             and the EEC for thiobencarb exceeds 1/10 the NOEC determined in a fish early
             life-stage study/invertebrate life-cycle study, thus triggering the  need for the study.
             Avian Subacute Toxicity Study[GLN71-2(b)];
             fvfo acute toxicity data are  available for a waterfowl species.   Therefore, an
             additional study must be submitted testing the avian dietary toxicity of technical
             thiobencarb to a waterfowl species, preferable the mallard.  The value added of
             these data is moderate.  There is a chance that the mallard may be more sensitive
             to thiobencarb than the bob white,  and this could change the conclusion of the risk
             assessment.  In general, however, the acute toxicity of thiobencarb is not a major
             concern.
                                          110
                                                iii in11 in in ill

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 •     Avian Reproduction Study [GLN 71-4(b)];  ,      -
       A core avian reproduction study has been submitted for an upland game species
       (the bobwhite), but only a supplemental study is available for a waterfowl species
       (the mallard). This supplemental data for the mallard indicates that it is the moire
       sensitive species and was thus used in the risk assessment.  The data requirement
       for an  avian reproduction study with  a  waterfowl  is still  outstanding.  The
       conclusion of the risk assessment is not dependent on these data since high risk
       could be concluded based on the results of the core study with the bobwhite.

 •     Seedling-Emergence Testing Study [GLN 123-1 (a)];
       The guideline  requirement for seedling  emergence  testing  is currently only
       partially fulfilled.  The test was classified supplemental for the two most sensitive
       species, lettuce and ryegrass,  because there was significant mortality of plants at
       the lowest test concentration. The Agency requests that additional testing be done
       for these.two sensitive species using lower test concentrations that do not result in
       mortality of plants. The value added of this information is moderate.  It would
       increase the confidence of the risk assessment on terrestrial  plants. Also, this
       information would be required  for comparative analysis of thiobencarb with other
      . herbicides.                  .

       2.     Labeling Requirements for Manufacturing-Use Products

       To remain in compliance with FIFRA, manufacturing  use product (MP) labeling
must be revised to comply with all current EPA regulations, PR Notices and applicable
policies.  The MP labeling must bear the following statement under Directions for Use:

       "Only for formulation into an Herbicide for the following use(s):rice weed control
       in California, Louisiana, Texas, Mississippi, Missouri and Arkansas, and lettuce,'
      .endive and celery weed control in Florida."

       An MP registrant may,  at his/her discretion, add one of the following statements
to an MP label under "Directions for Use" to permit the reformulation of the product for
a specific use or all additional uses supported by a formulator or user group:

       (a)     "This product may be used to formulate products for specific
              use(s) not listed on the MP label if the formulator, user group, or
              grower has complied  with U.S.  EPA submission requirements
              regarding support of such use(s)."

       (b)     "This  product  may be used to formulate products for  any
              additional use(s)  not listed on the MP label  if the formulator, user  -.
             'group,  or grower has  complied with U.S.  EPA submission
           .  requirements regarding support of such use(s)."
                                   111

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     1 ' < .(III : SB! ii) >i
                       tn:X';i>:ffifปiฃl ..... "ii i!1 ii!':1 I:!:U: '! ',!• i|i<|t I'X :,ซ ! T
                                                 '' SB i'll mi*! if* : :,* '"
       The reregistration requirements for magnitude of the residue in water will be
consider^ fulfilled when revisions are made to Valent's end-use product labels (EPA Reg.
Nos. 59639-79 and 59639-80) to prohibit use of treated water for livestock watering or
for drinking or irrigation for a specified time period after treatment.  Based on the results
of an  acceptable  magnitude  of residue  in  potable water  study  (MRIDs 43404003,
43404004, and 43404005), thiobencairb and thiobeiicarbsulfoxide residues in runoff and
receiving waters associated with rice fields did not fall to acceptable levels until 14 days
after treatment.                                                            ;

       The use of the thiobencarb granular formulation  (Boleroฎ 10G, EPA Reg. No.
59639-80) in!'California is ^^3^uncier the gasin Planfor'the Sacramento River Basin
established by the California Regional Water Quality Control Board, Central Valley
Region.  A performance goal of 1.5 ppb is strictly monitored, and growers must adhere
to a program of approved management  practices,  including a 30-day water holding
restriction.

       The reregistration requirements for nature and magnitude of the residue in fish will
be fulfilled when label revisions are made on Valent's end-use products (EPA Reg. Nos.
59639-79 and 59539:35) ^ spec,j|y ^ g^o^n^-~~-& restrictions:   "Do not use on rice
paddies where commercial catfish or crayfish farming is practiced.  Do  not use adjacent
to catfish or crayfish ponds."
              ii                                                 iซ  '.,•! '  ,  „'   '     , '!!,,,,',, ,
       Valent's thiobencarb end-use labels specify a 6-month plantback interval following
rice and all other crops, except celery, endive and lettuce for which rotational crop plant-
back intervals are 4-months.   These currently  specified  plant-back  intervals  are
appropriate.
 B.    End-Use Products

       1.      Additional Product-Specific Data Requirements
       mi i  i    i  i  mi i  n i i    i  i i  i  i i i  i  i   i     i in   mi               '  ,     '   . 	, ;
               Section 4(g)(2)(B) of FIFRA calls for the Agency to obtain any needed
       product-specific data regarding the pesticide after a determination of eligibility has
       been made. Registrants must review previous data submissions to ensure that they
       meet current EPA acceptance criteria and if not, commit to conduct new studies.
       If a registrant  believes  that previously submitted  data  meet current testing
       Standards, then study MRID numbers should be cited according to the instructions
       in the Requirement Status and Registrants Response Form provided for  each
       product.
        2.      Labeling Requirements for End-Use Products

        The labels and labeling of all products must comply with EPA1 s current regulations
 and requirements as specified in 40 CFR  ง156.10 and other applicable notices.
                                     112

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              a.     PPE and Engineering Control  Requirements for  Pesticide
                    Handlers

       For sole-active-ingredierit end-use products that contain thiobencarb:

       •      Revise the product labeling to adopt the handler personal protective
              equipment/engineering control requirements set forth in this section.

       •      Remove any conflicting PPE requirements on the current labeling.

       For multiple-active-ingredient end-use products  that contain thiobencarb:

       •      Compare the handler personal protective  equipment/engineering control
              requirements set forth in this section to the requirements on the current
              labeling.                                                       •

       •      Retain the more protective requirements. (For guidance  on which
              requirements are considered more protective, see PR Notice 93-7.)
       3.
Products Intended Primarily for Occupational Use

      Active-Ingredient Specific Engineering Control Requirements
              a.
       EPA is establishing  active-ingredient  specific  engineering controls  for  some
occupational uses of thiobencarb end-use products.

       For liquid formulations:               .

       "Mixers and loaders are required to use closed systems. The closed system
       must be used in a manner that meets the requirements listed in the Worker
       Protection  Standard  (WPS)  for   agricultural  pesticides  (40  CFR
       170.240(d)(4)."                                    '  .    '

       "Applicators and flaggers are required to use enclosed cabs or enclosed cockpits.
       The closed system must be used in a manner that meets the-requirements listed in
       the  Worker Protection Standard (WPS)  for agricultural  pesticides  (40 CFR
       170.240(d)(5-6)."

       4.      Active-Ingredient    Specific   Personal   Protective   Equipment
              Requirements

       EPA is establishing  active-ingredient  specific personal protective  equipment
requirements for all occupational uses of thiobencarb end-use products.
                                   113

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                  'li,'1'!,,*!!!, W iiilisl :'l!i''i; Hi!'1 <"' Hi	 ilh '''V' Ul "I!	;
                                               i1,,:1 ill"1:" Til: I" 'nilillMH1 I'll' r"! il,,; '
        For Uquid formulations:

        "In addition to using closed systems, mixers and loaders must wear:
        -- long-sleeved shirt and long pants,
        =rchemical-resistant gloves*,.
        —socks plus shoes, and
        .Chemical-resistant apron."

        "Applicators and flaggers using enclosed cabs or cockpits must wear:
        - long-sleeved shirt and long pants, and
        —socks plus shoes."
        I	Ill  MI    i    i  i    i     i| i    i i    i II il         i i I        ..... ! "L   '.  :     ,,   ••'• ' i1.
        Ill 111 I I           I I    I I  I  I   Ml     II I         I I .1      " !.  1   ,:  .'   ' ''.',   . • - i"
        ''tor other handling activities and in case of a spill or other emergency exposure,
        handlers must wear;	'	, .
        —coveralls over long-sleeved shirt and long pants,
        -^chemical-resistant gloves*,
        —chemical-resistant footwear, and
        —chemical-resistant apron when cleaning equipment."

        *Fbf the glove statement, use me statement established for thiobencarb through the
        instructions in Supplement Three of PR Notice 93-7.

        For granular formulations:

        "Applicators and other handlers must wear:
        = long-sleeved shirt and long pants,
        —chemical-resistant gloves*,
        — shoes plus socks
        —chemical-resistant apron when loading formulation into equipment or cleaning
.- .; "^\:. ."equipment."

        *For the glove statement, use the statement established for thiobencarb through the
        instructions in Supplement Three of PR  Notice 93-7.

        5.      Determining PPE  Labeling  Requirements  for  End-use  Products
               Containing This Active Ingredient

        The PPE that would be established on the basis of the acute toxicity category of the
end-use product must be compared to the active-ingredient specific personal protective
equipment specified above, the more protective PPE must be placed on the product
labeling. For guidance on which PPE is considered more protective,  see PR Notice 93-7.
                                    114

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       6.     Placement in Labeling

       The perso.nal protective equipment requirements must be placed on the end-use
product labeling in the location specified in PR Notice 93-7, and the format and language
of the PPE requirements must be the same as is specified in PR Notice 93-7.
  t  •                          ',,-""             -  '
       7.     Entry Restrictions

       For sole-active-ingredient end-use products that contain thiobencarb:

       •  .    Revise the product labeling to adopt the entry restrictions set forth in this
              section.

       •      Remove any conflicting entry restrictions on the current labeling.'

       For multiple-active-ingredient end-use products that contain thiobencarb:

       •      Compare the  entry restrictions set forth  in  this section to  the entry
       „       restrictions on the current labeling. "

       •      Retain the more protective restrictions. (A specific time period in hours or
              days is considered more protective than "sprays have dried" or "dusts have
            '  settled.")

       8.     Products Intended Primarily for Occupational Use

              a.     WPS Uses

                    (1)    Restricted-entry interval:

       A 24-hour restricted-entry interval (REI) "is required for uses within the scope of
the WPS on all thiobencarb end-use products.

                    (2)     Early-entry personal protective equipment (PPE):

              The PPE required for early entry is:
              — coveralls,
              — chemical-resistant gloves,
              — shoes plus socks,

              b.     Placement in labeling:

       The REI must be  inserted into the standardized REI  statement  required by
Supplement Three of PR Notice 93-7.  The PPE required for early entry must be inserted

                                   115

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.•!,'" I1 U'Jia i "||l|!l If ! ,
                   into the standardized early-entry PPE statement required by Supplement Three of PR
                   Notice 93-7.
                                       , ' ll'lllll! ..... .rill' '•", ,. I'l ...... , i,,l. "W 'I W'NI :l .......... Hi' '"IIP: Li' , .1.
                                                          1 'EM i, 'IT iiUliilllI'lil'l' ViiVS I'llf'L'L'11 'nlii'l" VI1 li'll	IHIT "iHT",.1,
                          Other Labeling Requirements

                          Application Restrictions
                          I I 111       I I  II I I  III        I II    I      II    II  I  I III I     i ,T '• . , . ,'!' ' ''    "     •   '  ,','•'
                          "Do not apply this product in a way that will contact workers or other persons,
                          either directly or indirectly or through drift. Only protected handlers may be in
                          the area during application. "

                          -Do not apply this product south of the Intracoastal Waterway in Louisiana."
                          f Do not apply this product within two  (2) miles  from the shorelines of Matagorda
                          Bay in Texas."
                          "Do not apply this product within two (2) miles from the shorelines of Galveston
                          Bay in Texas."
                          I IP          I  Ml III   ,1    III       I        I   I I I        '."".. ;!V  "    •;   ';'  ;^| .<•(/
                          ?Do not apply this product to rice fields with catfish/crayfish farming."
                          "Do not apply this product on rice fields adjacent to catfish or crayfish ponds."
                          "When applying to rice fields, do not release permanent flood water within 14-
                          days of application of this product (where weather permits)."

                          "Avoid application of this product within 24 hours of rainfall, or when heavy rain
                          is expected to occur within 24 hours."

                          "Do not mix/load or otherwise handle this product within 100 feet of aquatic
                          habitat."
                          User Safety Requirements

                          1.      Registrants: place the following statement on the labeling if coveralls are
                                 required for pesticide handlers on the end-use product label:
                                                                               ' ,  '!• '  ' i'|, '''   i,     ,'ป!'    ' j1
                                 "Discard clothing or other absorbent materials that have been drenched or
                                 heavily contaminated with this product's concentrate.  Do not reuse them."

                          2.      Registrants always place the following statement on the end-use product
                                 labeling:	:	"	

                                 "Follow manufacturer's instructions for cleaning/maintaining PPE.  If not
                                 such instructions for washables, use detergent and  hot water.  Keep and
                                 wash PPE  separately from other laundry."
                                                       116

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       User Safety Recommendations                                   ,

       •      "Users should wash hands before eating, drinking, chewing gum, using
              tobacco, or using the toilet." '

       •    '  "Users should remove clothing immediately if pesticide gets inside. Then
              wash thoroughly and put on clean clothing."

     •  •      "Users should remove PPE immediately after handling this product.  Wash
              the outside of gloves  before removing.-   As  soon as possible, wash
              thoroughly and change  into clean clothing."

C.     Spray Drift Labeling

The following language must be placed on each product label that can ,be applied aerially:

       Avoiding spray drift at the application site is the responsibility of the appk'cator.
       The interaction of many equipment-and-weather-related factors determine the
       potential for  spray drift.   The applicator and the grower  are responsible for
       considering all these factors when making decisions.

       The following drift management requirements must be followed to avoid off-target-
       drift  movement from aerial applications  to agricultural field crops.   These
       requirements  do not apply to forestry applications, public health  uses  or to
       applications using dry formulations.

              1.
              2.
              The distance of the outer most nozzles on the boom must not exceed
              3/4 the length of the wingspan or rotor.

              Nozzles must always point backward parallel with the air stream
              and never be pointed downwards more than 45 degrees.

Where states have more stringent regulations, they should be observed.
                       •           .                         ป

It is recommended that the applicator should be familiar with and take into account
the information covered in the Aerial Drift Reduction Advisory Information.

The following aerial drift reduction advisory information must be contained in the
product labeling:                           .

       [This section is advisory in nature and does not supersede the mandatory
       label requirements.]
                                   117

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f1:1!'1 ..... ri'
:]IIB

I" !>'1{; j	ipi
 INFORMATION ON DROPLET SIZE:  The most effective way to reduce drift
 potential is to apply large droplets.  The best drift management strategy is to apply
 the largest droplets that provide sufficient coverage and control.  Applying larger
 droplets reduces drift potential, but will not prevent drift if applications are made
 improperly,   or  under unfavorable  environmental  conditions  (see Wind,
 Temperature and Huniid'ityV11^^"^eniperafure Inversions).

 " /'^/iivrnnDf\ฅTTTVT^"* T^TD^^TDirT7TT1 Ch tii*•
 • • Vซf\fr,L^( JL JtvV/JL/JL/JLli i vJ J^9,t^\_ffr M^SL*J f >J_I..F-*•?/ *

 •     Volume - Use high flow rate nozzles to apply the highest practical spray
 volume. Nozzles with higher rated flows produce larger droplets.

 •     Pressure  -  Do not,exceed  the nozzle manufacturer's recommended
 pressures.  For many nozzle types lower pressure produces  larger droplets.  When
 Jjigher flow rates are needed,  use higher flow rate nozzles instead of increasing
 pressure.

 •     Number of nozzles - Use the minimum number of nozzles that provide
 uniform coverage.

 •     Nozzle Orientation - Orienting nozzles so that the spray is released parallel
 to  the airstream,produces larger droplets than other orientations and  is the
 lecommended practice.  Significant deflection from horizontal will reduce droplet
 size and increase drift potential.
 Illl Ill I             I        II I III I I I   I I I I  II    III II    III      ,• T  f'l ,  ' ''!    '   •  i* , •'  •• „' •' ' '
 •     Nozzle Type  - Use a nozzle type that is  designed  for  the  intended
 application.   With most  nozzle types, narrower spray  angles produce larger
 ^drpplets.  Consider using low-drift nozzles.  Solid stream nozzles oriented straight
 back produce the largest droplets and the lowest drift.

 i      Maintainance of Nozzles - periodic inspection and subsequent replacement
 of nozzles to ensure proper chemical application is recommended.
 II II             I         II        I I   I  I I      II         '   , i  ' • '  i          "  . '
 BOOM LENGTH: For some use patterns, reducing the effective boom  length to
 less than 3/4 of the wingspan or rotor length may further reduce drift without
 reducing swath width.

 APPLICATION HEIGHT: Applications should not be made at a height greater
 than 10 feet above the top of the largest plants  unless a greater  height is required
 for aircraft safety.  Making applications at the lowest height that is safe reduces
 exposure of droplets to evaporation and wind.

 SWATH ADJUSTMENT: When applications are made with a crosswind, the
 swath will be displaced downward.  Therefore, on the up and downwind edges of
                              118
                             D!" S,	IV.l'i'f	lt!\;;i"!:}'!!ป

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       the field, the applicator must compensate for this displacement by, adjusting the
       path of the aircraft upwind.  Swath adjustment distance should increase, with
       increasing drift potential (higher wind, smaller drops, etc.)

       WIND: Drift potential is lowest between wind speeds of 2-10 mph. However,
       many factors, including droplet size and equipment type determine drift potential
       at any given speed.  Application should be avoided below 2 mph due to variable
       wind direction and high inversion potential.  NOTE:  Local terrain can influence
       wind patterns.  -Every applicator should be familiar with local wind patterns and
       how they affect spray drift.                             ,

       TEMPERATURE AND HUMIDITY: When making applications in low relative
       humidity,  set  up  equipment  to, produce larger droplets  to compensate  for
       evaporation. Droplet evaporation is most severe when conditions' are both hot and
       dry.

       TEMPERATURE INVERSIONS:  Applications should not occur during a
       temperature inversion  because drift potential, is high.  Temperature inversions
     ,  restrict vertical air mixing, which causes small suspended droplets to remain in a
       concentrated "cloud. This cloud can move in unpredictable directions due to the
     .  light variable winds common during inversions.  Temperature inversions  are
       characterized by increasing temperatures with altitude and are common on nights
       with limited cloud cover and light to no wind.  They begin to form as the sun sets
       and often continue into the morning.  Their presence-can be indicated by ground
       fog; however, if fog  is not present, inversions can also be identified by  the
       movement of smoke from a ground source or an aircraft smoke generator. Smoke
       that layers and moves laterally in a concentrated cloud (under low .wind conditions)
       indicates an inversion, while smoke that moves upward and rapidly dissipates
       indicates good vertical  air mixing.     .                      .

       SENSITIVE AREAS:  The pesticide should only be applied when the potential for
       drift to adjacent sensitive  areas (e.g. residential areas, bodies of water, known
       habitat for threatened or endangered1 species, non-target crops) is minimal (e.g.
      'when wind is:blowing away from the sensitive areas).

D.     Existing Stocks

             Registrants may generally distribute  and  sell  products  bearing old
       labels/labeling for 26 months from  the date of the issuance of this Reregistration
       Eligibility Decision (RED). Persons  other than the registrant  may generally
       distribute or sell'such products for 50 months from the date of the issuance of this
       RED. However,  existing  stocks time frames will be established case-by-case,
       depending on the number of products involved, the number of label  changes, and
                                  119

-------
other factors.  Refer to  "Existing  Stocks  of Pesticide Products; Statement of
Policy": Federal Register. Volume 56, No. 123, June 26, 1991.

        The Agency has determined  that registrants may distribute and  sell
thiobencarb products bearing old labels/labeling for 26 months from the date of
issuance of this  RED.  Persons other than the registrant may distribute or sell such
products for 50 months from the date of the issuance of this RED.  Registrants and
persons other  than registrants  remain obligated to meet pre-existing Agency-
imposed label changes and existing stocks requirements applicable to products they
sell or distribute.
                             120

-------
VI. APPENDICES

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-------
                               GUIDE TO APPENDIX B
Appendix B contains listings of data requirements which support the reregistration for active
ingredients within the case 2665 covered by this Reregistration Eligibility Decision Document.
It contains generic  data requirements  that apply  to  2665 in all products,  including data
requirements for which a "typical formulation" is the test substance.            ...

       The data table is organized in. the following format:

       1., Data Requirement (Column 1). The data requirements are listed in the order in which
they appear' in 40 CFR Part 158.  the reference numbers accompanying each  test refer to the test
protocols set in the  Pesticide Assessment Guidelines, which are available from the National
Technical Information Service, 5285 Port Royal.Road, Springfield, VA 22161 (703) 487-4650.

       2.  Use Pattern (Column 2).  This column indicates the use patterns for which the data
requirements apply.  The following letter designations are used for the given use patterns:
                                          ,           i               '
               ,            A     Terrestrial food       .            ,
                           B     Terrestrial feed
                   ,        C     Terrestrial non-food
                           D     Aquatic food
                           E     Aquatic non-food outdoor
                           F     Aquatic non-food industrial                          ;
                        '   G     Aquatic non-food residential
                           H     Greenhouse food
                           I     Greenhouse non-food   .-       .
                 .        .  J     Forestry
                           K     Residential
                           L     Indoor food
                           M     Indoor non-food
                           N     Indoor medical       -          •
                           O     Indoor residential

       3.  Bibliographic citation (Column 3).  If the Agency has acceptable data in its files, this
column lists, the  identifying  number of each study.   This normally is the Master  Record
Identification (MRID) number,  but may be a "GS" number if no MRID number has been
assigned.  Refer to the Bibliography appendix for a complete citation of the study.
                                          129

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130

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                      APPENDIX B

Data Supporting Guideline Requirements for the Reregistration of
Thiobencarb
REQUIREMENT
USE PATTERN CITATIONCS)
PRODUCT CHEMISTRY
61-1 .
61-2A .
61-2B
62-1
62-2
62-3
63-2
63-3
63-4
63-6
63-7
63-8 .
63-9
63-11 .
63-12
63-13
Chemical Identity
Start. Mat. & Mnfg. Process
Formation of Impurities
Preliminary Analysis
Certification of limits
Analytical Method
Color
Physical State
Odor . '
Boiling Point
Density
Solubility
Vapor Pressure
Octanol/Water Partition
PH •
Stability , '
ALL
ALL
ALL
ALL
ALL
ALL
ALL
ALL
ALL.
ALL
ALL
ALL
ALL
ALL
ALL
ALL
41609003
44507, 41609001
44507, 41609001
44507, 41609002
140158, 41609002
44507, 41609002
44507, 41609002
44507, 41609002
44507, 41609003
140158 ,
44507
140158
140158
44507
44507
44507
ECOLOGICAL EFFECTS
71-1A
71-2A
71-2B
71-4A
71-4B
72-1A
72-1B
72-1C
72-1D •
Acute Avian Oral - Quail/Duck
Avian Dietary - Quail
Avian Dietary - Duck
Avian Reproduction - Quail
Avian Reproduction - Duck
Fish Toxicity Bluegill
Fish Toxicity Bluegill - TEP
Fish Toxicity Rainbow Trout
. Fish Toxicity Rainbow Trout- TEP .'
ALL
ALL
B, D
B, D
B,D
B, D
B, D
ALL
B, D
43121201 ,
55224
57225 .
43075401
25778 .
50665
50665
50664
50664
                              131

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Data Supporting Guideline Requirements for the Reregistration of
Thiobencarb
REQUIREMENT
USE PATTERN
CITATION(S)
72-2A      Invertebrate Toxicity                          ALL
72-2B      Invertebrate Toxicity-TEP                     B,D
72-3A      Estuarine/Marine Toxicity - Fish                B, D
72-3B      Estuarine/Marine Toxicity - Mollusk             B, D
72-3C      Estuarine/Marine Toxicity - Shrimp              B, D
72-3D      Estuarine/Marine Toxicity Fish- TEP             B, D
72-3E      Estuarine/Marine Toxicity Mollusk - TEP         B, D
72-3F      Estuarine/Marine Toxicity Shrimp - TEP         B, D
72-4A      Early Life Stage Fish                          B~' D
72-4B      Life Cycle Invertebrate                        B, D
72-6       Aquatic Organism Accumulation                 B, D
122-lA     Seed Germination/Seedling Emergence           B, D
122-1B     Vegetative Vigor                             B[, D
122:2      Aquatic Plant Growth                         B, D
123-1A     Seed Germination/Seedling Emergence           B, D
123-lB	Vegetative Vigor	  B,	D
123-2      ASua,ti? Plant Growtn                         ?. P.,
TQXICC)LOGf ''' ' '  '	''";;': " 	''"; :'  :	::":"''":'	:::	"'' ''";'":';::	
81-1       Acute Oral Toxicity - Rat                       ALL
81-2       Acute Dermal Toxicity - Rabbit/Rat              ALL
81-3       Acute Inhalation Toxicity - Rat                  ALL
81-4       Primary Eye  Irritation - Rabbit                  ALL
81-5       Primary Dermal Irritation - Rabbit               ALL
81-6       Dermal Sensitization - Guinea Pig                ALL
81-7       Acute Delayed Neurotoxicity - Hen              ALL
8l-f-SS     Acute Neurotoxicity
82-2       21~Davn Dermal - Rabbit/Rat                    ALL
82-4       90-Day Inhalation - Rat                        ALL
82-7-SS     90-Day Neurotoxicity

   '	••'	 """  '	• •   '"	'	•''•'	- ' •	."""'•'-'	132
                 25788
                 40031001
                 79110, 79112
                 79114
                 50667,79117
                 79111
                 79115
                 79113
                 79112
                 42680401
                 133563
                 41690902
                 41690902
                 41690901,41690902
                 41690902
                 41690902
                 41690901, 41690902

                 42130701
                 42130701
                 40585, 134976
                 40581
                 40583, 81900
                 161699
                 42987001, 43148202
                 42987001
                 42893001
                 42893001
                 43001001

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Data Supporting Guideline Requirements for the Reregistration of
Thiobencarb
REQUIREMENT
83-1 A
83- IB
83-2A
83-2B
83-3A
83-3B
83-4
84-2A
84-2B
84-4
85-1
85-2 .
Chronic Feeding Toxicity - Rodent
Chronic Feeding Toxicity - Non-Rodent
Oncogenicity - Rat
Oncogenicity - Mouse •
Developmental Toxicity - Rat
Developmental Toxicity - Rabbit
2-Generation Reproduction - Rat
Gene Mutation (Ames Test)
Structural Chromosomal Aberration
Other Genotoxic Effects
General Metabolism /
Dermal Penetration
USE PATTERN CITATiON(S)
ALL
ALL
ALL
ALL
. ALL
ALL
ALL
ALL
ALL
ALL
ALL
ALL
154506 ' ' '
144742
154506
86004
86873, 93691,
115248
164313 :
40446201, 40985701
135285, 84131,
41174
84133, 40352401
40352401
' 42340302
41215311
ENVIRONMENTAL FATE . ' . . ' . ' . •.,.••
160-5
161-1
161-2
161-3
162-1
162-3
162-4
163-1
164-1
164-2
165-2
165-3 '
165-4
201-1
202-1
Chemical Identity •
Hydrolysis
Photodegradation - Water
Photodegradation - Soil
Aerobic Soil Metabolism
Anaerobic Aquatic Metabolism
Aerobic Aquatic Metabolism
Leaching/ Adsorption/Desorption
Terrestrial Field Dissipation
Aquatic Field Dissipation
Field Rotational Crop *
Accumulation - Irrigated Crop
Bioaccumulation in Fish
Droplet Size Spectrum
Drift Field Evaluation
ALL
,B, D
B, D
B
B
B, D
D
B,D
B
D
B
D
B,D
B, D
B,D
41609003
41609012
42257801
41215312
43300401
43252001
42052001
43150601 ,
43404004, 42003405
43404004, 42003405
41609011
43148201
42460401
TASK FORCE
TASK FORCE
                                 133

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                                                                                            : ,'!!. '„ :	:;i T"'1'" I':;1"'::,!!1!1' ! 'OMI'i*1'
      Ijlrilj|i|!;, ^a||,gupporting Guideline	Requirements for	the Registration of
         Thiobencarb
         REQUIREMENT
                                                     USE PATTERN
                                                                                    CITATION(S)
:-; ":„,-,::,: • RESIDUE CHEMISTRY
 M;!'l tl;;!f ! . .; ' PHI	W. ' a'iSr'1""1!"!1:",!";;'	:•	lilllli: m-K	i.'M:	S'VS'JW C fi! i' f f !'} ' E I ',iif;i! * rM ' <; •!" I ':
                     Residue Analytical Method - Animal
                     Storage Stability
                     Magnitude of Residues - Potable H2O
                     Magnitude of Residues - Irrigated Crop
                    i      i iiil          i  i
                     Magnitude of Residues -
                     Meat/Milk/Poultry/Egg
                     Crop Field Trials
                          iiiii  i
                     Processed Food
                                                   V, >i .......... f-iyr
                                                   ;ii: ..... ('.:.'• ,!:• j 1 1
                                                                 -'M ...... :•' .':•. iWlBl!,,1!!;1 !•'
                                                                 , Wl, ......... l/'iC!1 'j
                                                                   ALL .....
                                                                   B
                                                                  B,D
                                                                 ^'J'IM^i:'
                                                                   B"
                                                                  ALL
                                                                   D
                                                                   D
                                                                 i
                                                                   B

                                                                  B,D
                                                                    i i
                                                                  ALL
4234Q301
43492301
43075402
92182073
43182501
43404003,43404004
124278
42962801, 42962802

92182080
42987002
                                                                                                  •v  if
I!!!' ป i; " I I	'•
                                                   134

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                           GUIDE TO APPENDIX C

 CONTENTS OF BIBLIOGRAPHY.   This bibliography  contains citations of all studies
 considered relevant by EPA in arriving at the positions and conclusions stated elsewhere, in the
 Reregistration Eligibility Document.  Primary sources for studies in this bibliography have been
 the body of data submitted to EPA and its predecessor agencies in support of past regulatory
 decisions. Selections from other sources including the published literature, in those instances
 where they have been considered, are included.

 UNITS OF ENTRY. The unit of entry in this bibliography is called a "study". In the case of
 published materials, this corresponds closely to an article.  In the case of unpublished materials
 submitted to the Agency, the Agency has sought to identify documents at a level parallel to the
 published article from within the typically larger volumes in which they were submitted.  The
 resulting "studies" generally have a distinct title (or at least a single-subject),,can stand alone for
 purposes of review and can be described with a conventional bibliographic citation. The Agency
 has also attempted to unite basic documents and commentaries upon them, treating them as a
 single study.

 IDENTIFICATION OF ENTRIES.  The entries in this bibliography are sorted numerically by
 Master Record Identifier, or "MRID number". This number is unique to the citation,  and
 should  be. used whenever a specific reference is required.  It is not related  to the six-digit
 "Accession Number"  which has been used  to identify volumes of submitted studies  (see
 paragraph 4(d)(4) below for .further explanation).  In a few  cases,  entries added to the
 bibliography late in the review may be preceded by a nine character temporary identifier. These
 entries are listed after all MRID entries.  This temporary identifying number is also to be used
 whenever specific reference is needed.

 FORM OF ENTRY. In addition to the  Master Record Identifier  (MRID), each entry consists
 of a citation containing standard elements followed, in the case of material submitted to EPA,.
 by a description of the earliest known submission.  Bibliographic conventions used reflect the
 standard of the American National Standards Institute (ANSI), expanded to provide  for certain
 special needs.

 a.     Author. Whenever the author could confidently  be identified, the Agency has chosen to
       show a personal author.  When no individual was identified, the Agency has shown an
       identifiable laboratory or testing facility as the author. When no author or  laboratory
       could be identified, the Agency has shown the first submitter  as the author.

b.     Document date.  The date of the  study is taken  directly from the document.  When the
       date is  followed by a question mark, the  bibliographer has deduced the date from the
       evidence contained in the document.  When the date appears as (19??), the Agency was
      unable to determine or estimate the date of the document.
c.
Title. In some cases, it has been necessary for the Agency bibliographers to create or
enhance a document title.  Any such editorial insertions are contained between square
brackets.                .
                                     135

-------
         Trailing parentheses.  For  studies submitted to the Agency in the past,  the trailing
         parentheses include (in addition to any self-explanatory text) the following elements
         deipribing the earliest known submission:
         (1)
         (2)
         (3)
         (4)
 Submission date.  The date of the earliest known submission appears immediately
 following the word "received."

 A3ministratiYง number..  The next element immediately following the word
 "under" is the registration number, experimental use permit number, petition
 number,  or other  administrative number associated with the earliest known
 submission.
III
ill;' :::!],:! ';•:" ffi1,: , svch!	i!!!;!S, 13 iiiS:	'ซii:P't! Irif!:';:i!:,i/;	':-siillp-ilfi!1,!!*'	r i!,'!!ty.&i,'•<•>'; ; i:'. .'*'.,'  '   "  •   '  •• 1' ':i';!,
 Submitter. The third element is the submitter.  When authorship is defaulted to
 the submitter, this element is omitted.

 Volume Identification (Accession Numbers).  The final element in the trailing
 parentheses identifies the EPA accession number of the volume in which the
 original submission of the study appears. The six-digit accession number follows
 the symbol "CDL," which stands for "Company Data Library." This accession
 number is in turn followed by an alphabetic suffix which shows the relative
 position of the study within the, volume.
              liEUf
              !l	lit!
                                         136

-------
                                     BIBLIOGRAPHY
     MRID
CITATION
00025778    Beavers,  J.B.; Fink, R.;  Joiner,  G.; et  al.  (1979) Final Report:  One-Generation
           , Reproduction Study-Mallard Duck: Project No.  162-117.  (Unpublished study including
             letters dated Apr 10, Apr 25, May 25, Jul 9, Jul 24, Aug 28, 1979 from J, Grimes, J.B.
             Beavers,  J.B. Leary, J. Grimes, J. Grimes, J.  Grimes, respectively, to Francis X.
             Kamienski and addendum, received Dec 11, 1979 under 239-2450; prepared by Wildlife
             international,  Ltd., submitted by Chevron Chemical Co., Richmond, Calif.; CDL:
             241489-G)

00025788    Wheeler, R.E. (1978) 48 Hour Acute Static Toxicity of Bolero (SX796) to 1st Stage
             Nymph Water Fleas (Daphnia magna Straus). (Unpublished study received Dec 11, 1979
             under 239-2450;  submitted by   Chevron  Chemical  Co.,  Richmond,  Calif.;
             CDL:241489-W)

00040581    Rittenhouse, J.R.; Narcisse, J.K. (1974) S-717: The Eye Irritation Potential of Bolero
             Technical: SOCAL 653/XX: 106.  (Unpublished study received Mar 18, 1976 under
             239-EX-78; submitted by Chevron Chemical Co., Richmond, Calif.; CDL:095493-P)

00040583    Rittenhouse, J.R.; Narcisse, J.K. (1974) S-718: The Skin Irritation Potential of Bolero
             Technical: SOCAL 654/XX:106.  (Unpublished study received Mar 18, 1976 under
             239-EX-78; submitted by Chevron Chemical Co., Richmond, Calif.; CDL:095493-R)

00040585    Narcisse, J.K. (1976) S-959: The Acute Inhalation  Toxicity of Bolero Technical Vapor:
             SOCAL 885/XXI:r48.  (Unpublished study received Mar 18, 1976 under 239-EX-78;
             submitted by Chevron Chemical Co., Richmond,  Calif.; CDL:095493-T)

00041174    Shirasu,  Y.;  Moritani, M.;  Kato,  K, (1974) Test  Report on  Mutagenicity  of
             S-(4-Chloroben2yl)-N,N-diethylthiolcarbamate in Microorganisms.  (Unpublished study
             received  Mar 18, 1976  under 239EX-78;  prepared  by  Institute  of  Inbiomental
             Toxicology, submitted by Chevron Chemical Co., Richmond, Calif.; CDL:095491-D)

00044507    Leary, J.B. (1976) Benthiocarb-Distribution between n-Octanol and Water: File No.
             741.11.  (Unpublished study received Mar 18,  1976 under 239-2449; submitted by
             Chevron Chemical Co., Richmond, Calif.; CDL:095095-N)

00050664    Thompson, C.M.; Griffen, J.; Boudreau, P.;  et al.  (1980) Acute Toxicity of Bolero 10G
             (SX-1252) to  Rainbow Trout (Salmo gairdneri):  S-1819:  ABC Report #  26078.
             (Unpublished study received Oct 23, 1980 under 239-2449; prepared by Analytical Bio
             Chemistry Laboratories, Inc., submitted by Chevron Chemical Co., Richmond, Calif.;
             CDL:243574-B)
                                          137

-------
                                    BIBLIOGRAPHY
     MRID
                        CITATION
00050665
00050667
    !WI|"i"" 	.' 'II si
00057224
00057225
00079110
00079111
00079112
00079113
Thompson, C.M.; Griffen, L; Boudreau, P.; et al. (1980) Acute Toxicity of Bolero 10G
(SX-1252) to Bluegill Sunfish (Lepomis macrochirus): S-1820: ABC Report # 26077.
(Unpublished study received Oct 23, 1980 under 239-2449; prepared by Analytical Bio
Chemistry Laboratories, Inc., submitted by Chevron Chemical Co., Richmond, Calif.;
CDL:243574-C)

Hollister, T.A. (1980)  Acute Toxicity of Boleroฎ = Technical to  Mysid Shrimp
'(Mysi^^'ba^:^^No. BP-80:9-16L oSn^ubh'shed study received Oct 23, 1980
under 239-2449; prepared by• EG"&G"Bionomics^ submitted by Chevron Chemical Co.,
Richmond, Calif.; CDL:243574-E)
      I                                              '               ",',•'

Fletcher, D. (1974) Report to Chevron Chemical Company, Ortho Division:  8-Day
Dietary LC50 Study with Bolero in Bobwhite Quail: IBT No. 651-05214.  (Unpublished
study received Mar  18,  1976  under 239-2449; prepared by  Industrial Bio-Test
Laboratories, Inc.,  submitted by Chevron Chemical Co., Richmond,  Calif.; CDL:
095494-H)

Fletcher, D. (1974) Report to Chevron Chemical Company, Ortho Division:  8-Day
Dietary  LC50  Study with Bolero in Mallard  Ducklings: IBT No. 651-05213.
(Unpublished study received Mar 18, 1976 under 239-2449; prepared by Industrial
Bio-Test Laboratories, Inc., submitted by Chevron Chemical Corp., Richmond, Calif.;
CDL:095494-I)

HeitmuUer, T. (1979) Acute Toxicity of Bolero Technical to  Sheepshead Minnows
(.Cyprinodon variegatus): Report No. BP-79-9-133. (Unpublished study received Dec 11,
1979 under  239-2449; prepared by EG & G,  Bionomics, submitted by Chevron
Chemical Co., Richmond,  Calif.; CDL:241494-L)

HeitmuUer, T. (1979) Acute Toxicity of Bolero 8 EC to Juvenile Sheepshead Minnows
(Cyprinodon variegatus): Report No. BP79-9-134. (Unpublished study received Dec 11,
1979 under 2392449; prepared by EG & G, Bionomics, submitted by Chevron Chemical
Co., Richmond, Calif.; CDL:241494-M)
Ward, G.S.  (1979) Effects  of Boleroฎ  = Technical on  Survival,  Growth, and
Development  of Sheepshead  Minnows   (Cyprinodon  variegatus):  Report  No.
BP-79-9-140,  (Unpublished study recieved Dec 11, 1979 under 239-2449; prepared by
EG & G,  Bionomics,  submitted by  Chevron  Chemical  Co.,  Richmond,  Calif.;
CDL:241494-N)
Heitmulier, T. (1979) Acute Toxicity of Bolero 8 EC to Fiddler Crabs (Uca pugilatof):
Report No. BP-79-9-135.  (Unpublished study received Dec 11, 1979 under 239-2449;
prepared by EG & G, Bionomics, submitted  by Chevron Chemical Co., Richmond,
Calif.; CDL:241494-P)
                                          138

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                                     BIBLIOGRAPHY
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                         CITATION
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 Hollister, T.A. (1979) Acute and Chronic Toxicity of Boleroฎ = Technical to Mysid
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 Rittenhouse,  J.R.; Narcisse, J.K. (1974) The  Skin  Irritation Potential of Bolero
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 Bullock, C.H. (1977) The Potential of Benthiocarb Technical and Benthiocarb Standard
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 Bootman, J.; Whalley, H.E. (1978) Boleroฎ = (Thiobencarb): Dominant Lethal Study
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 (Unpublished study received Dec 11, 1979 under 6F1763;  prepared, by Life Science
 Research,  England,  submitted by  Chevron  Chemical Co.,  Richmond,  Calif-
 CDL:099125-I)            .                                                 '

 Macrae, S.M.; Amyes, S.J.; Holmes, P.; et al. (1981) Technical Boleroฎ =: Potential
 Oncogenicity  in Dietary Administraiton to Mice:  LSR Report No.  81/KCI040/527.
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 Science Applications, Incorporated (1981) Teratology Study in Rats with Boleroฎ =:
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by Chevron Chemical Co., Richmond, Calif.; CDL:070494-A)

 Chevron  Chemical Company (1981) Additional Data for the Teratology Study in Rats
with Bolero: Ortho Test No. S-1947.  (Unpublished study, including letter dated Jan 11,
 1982  from J.A.  Parker  to  D.F. Dye, received Jan 15, 1982  under OF2322-
CDL:070594-A)
                                          139

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     Ill II11
     MRID
                         BIBLIOGRAPHY

                         CITATION
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  Pill   III I I II III   I    I   II     I   III          I I II    I    III II I I       ' ' ' .  ''I .       ' „  "  ฐ
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11 !ป; JI, i|l|;:il ',1	1:,, 'I'll'' IIIIIIIIIIIIIIJ1' I, J.'J]'1!1:™!;!!!!;1.1 iillll, I ,,l! ;| ""i lill'"!;',,!; "in, 'Ini !l,ii!;!l!! i 'i • lilliiE" !ii,i :,i,
-------
                                     BIBLIOGRAPHY
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             Proj. ID 8616165. Unpublished study prepared by Rio Palenque Research Corp.  31 p.

40352401     Boatman,  J.; Hodson-Walker, G.; Dance, C. (1985) In Vitro  Assessment of the
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             33p.                                                            /

40446201     Hatakeyama,  Y. (1987) Reproduction Study  by  Oral  Forced  Administration of
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40985701     Richter, W. (1988) A Discussion of Issues Related to Systemic NOEL and Reproductive
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41215301     Mayer, F.; Elersieck, M. (1986) Manual of Acute Toxicity: Interpretation and Data Base
             for 410 Chemicals and 66 Species of Freshwater Animals.  Unpublished study prepared
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41215311     Brorby, G.; Beatty, P. (1989) The Percutaneous Absorption of (Carbon 14)-Bolero 8 EC
             in  Male Rats: Project  ID  S-3145.   Unpublished  study  prepared by  Chevron
             Environmental Health Center, Inc. 47 p.

41215312     McGovern, P. (1988) Soil Surface Photolysis of [Carbon 14]-Thiobencarb in Natural
             Sunlight: Project ID MEF-0010. Unpublished study prepared by the Pharmacology and
             Toxicology Research Laboratory. 89 p.                         "

41609001     Updyke, J. (1990) Thiobencarb  Technical Product  Chemistry: Product Identity and
             Composition: Lab Project Number: 9009745. Unpublished study prepared by Chevron
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41609002     Updyke, J. (1990) Thiobencarb Technical Product Chemistry [Analysis and Certification
             of Product Ingredients]: Lab Project Number: 9009746. Unpublished study prepared by
             Chevron Chemical Co. 79 p.                               •

41609003     Updyke, J. (1990) Thiobencarb Technical Product Chemistry [Physical and Chemical
             Characteristics]: Lab  Project Number: 9009747.   Unpublished  study prepared by
             Chevron Chemical Co. 39 p.
                                           141

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     41690901
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     42015301



     42130701



     42257801



     42340301



     4234Q3Q2



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                                       '<. iviif,., '',,•' I"1!1 , "i!1 	 '/iniB/'iii	i1 MMji'LJi .• "I!1! H"1 „!,,
                                                                 I'll ,i|i"I!1 "I!	'•,' ,
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 [Field Dissipation Study]: Lab Project Number: VC7403. Unpublished study prepared
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 Giddings, J. (1990) Thiobencarb Technical-Toxicity to Five Species of Aquatic Plants:
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 Hoberg, J.   (1990)  Thiobencarb  Technical:  Determination  of Effects  on Seed
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' Inc. 170 p.	

 Lai, J.  (1991)  Field Dissipation  of Bolero 8EC in Rice:  Lab Project Number:
 1641/90/7538. Unpublished study prepared by Chevron Chemical Co. 411 p.

 Mulkey, N. (1991) The Aerobic Aquatic Metabolism of [Ring-Carbon 14]-Thiobencarb:
 Lab Project Number: ADC 1238.   Unpublished study prepared by  Analytical
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 Nishimura,  N. (1985) Acute Toxicity  Study of Benthiocarb  by Oral and Dermal
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 Chen,  Y. (1988) Photodegradation of [Phenyl-U-14C]-Thiobencarb in Water: Lab
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 Downs,  J.;  Marsh,  D.;  Krautter,  G. (1992) The Metabolism of [Ring-carbon  14]
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 PTRLJBast, Inc. and PTRL West, Inc.  371 p.
  1 • 1, ' , il'IFillillliJ '           III      II      I    I     I I I  III      ;' '„, •'  ' ' ' ' -i •  .  •   '  "   •  ,  '"
 Thacker,  J.; Strauss, K.; Smith, G. (1992) Thiobencarb: A Metabolic Fate Study with
 the Bluegill (Lepomis macrochirus): Lab Project Number: 263E-101. Unpublished study
 prepared by Wildlife International Ltd.  165 p.
                                               142
                       i nil

-------
                                     BIBLIOGRAPHY
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42680401     Putt, 'A. (1993) Thiobencarb (Bolero Technical): The Chronic Toxicity to Daphnia
             magna   under  Flow-through  Conditions:  Lab   Project  Number:   93-1-4582:
             12707.0792.6116.130. Unpublished study prepared by Springborn Labs,  Inc. 98 p.

42893001     Machado, M. (1993) Three-Week Repeated-Dose Dermal Toxicity Study in Adult Male
             and Female Rats with BOLERO SEC (SX-1843) MRID 42003401: Revised Report
             Number One: Lab Project Number: 5510.  Unpublished study prepared by Chevron
             Research & Technology Co.  391 p.

42962801     Lai, J. (1993) Meat and Milk Magnitude of the Residue Study in Lactating Dairy Cows
             with Thiobencarb: Lab Project Number:  VP10015:  133-001-10:  1714-92-10015.
             Unpublished study prepared by Valent U.S.A. Corp.  506 p.

42962802     Lai, J. (1993) Phase 3 Reformat of Thiobencarb Meat and Egg Poultry Feeding Study
             MRED 92182075: Response to Comments Upon Review: Lab Project Number: T3481R.
             Unpublished study prepared by Valent U.S.A. Corp.  13 p.

42987002     Lai, J. (1993) Magnitude of Thiobencarb Residues in Processed Rice Commodities: Lab
             Project Number: V1048: 1714-92-1048. Unpublished study prepared by Valent U.S.A.
             Corp. Dublin Lab. 200 p.               ,             ,

43001001     Lamb, I. (1993) A Subchronic (13-Week) Neurotoxicity Study of Bolero Technical in
             Rats: Lab Project Number:  WIL/194011:  194011:  VP/10008.  Unpublished study
             prepared by WIL Research Labs., Inc. 1634 p.

43075401     Bavers,  J.; Chafey, K.; Mitchell, L.;  et al. (1993) A Reproduction Study With The
             Northern Bobwhite: Lab Project Number: 263-128.  Unpublished study prepared by
             Wildlife International Ltd.  188 p.

43075402     Hughes, D. (1993) FDA Multiresidue Method Testing of Thiobencarb Metabolites:
             4-Chlorobenzylmethylsulfonej 4-Chloroben2ylmethylsulfoxide, and 4-Chlorobenzoic
             Acid: Lab Project Number: HWI 6320-116.  Unpublished study prepared by Hazleton
             Wisconsin, Inc. 143 p.
                                                  i                         •
43121201     Schocken, M. (1994) .(carbon 14) TMobencarb-Determination of Adsorption/Desorption
             Coefficients  for Soil Degradates:  Lab  Project  Number: VP-10011:  93-12-5074.
             Unpublished study prepared by Springborn Laboratories, Inc.  71 p.

43148201     Green, C. (1994) Accumulation of Thiobencarb and Metabolites in Crops Irrigated with
             Water Treated with BOLERO 8 EC Herbicide: Lab Project Number:  V/93/10610:
             3/288/0859: 3/288/0861. Unpublished study prepared by Valent U.S.A.  Corp., A&L
             Mid West Lab., Inc., Chemtec.  381 p.
                                           143

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                            •'iir'! , :n	'  t,:	'.,:,,";' ,,i	Mi1 INI inn	''"11	••Unii,•'• ,:fijni "if,*1: i'  ' ih '..iiiWii'Tii	:• "
43148202
43150601
43182501
43252001
43300401
43404003
43404004



43404005


43492301



92182073
92182080
  Lamb, I. (1994) A Range-Finding Study of BOLERO Technical in Rats: Lab Project
  Number: WIL/194009. Unpublished study prepared by WIL Research Lab., Inc. 206
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  Christensen, K, (1994) 4-Chlorobenzoic Acid-Determination of the Adsorption and
  Besprption Properties:  Lab  Project Number: 12709.1093.6132.710:  93-11-5051:
  VP-10804. Unpublished study prepared by Springborn Labs., Inc.  73 p.
  Ray, D. (1994) Storage Intervals and Conditions for Samples from Magnitude of the
  Residue Studies Conducted in Support of Thiobencarb Raw Agricultural Commodity
  Tfiterงness: Lab Project Number: 94-THI-01. Unpublished study prepared by Valent
  U.S.A. Corp. 30 p.

  Esser, T.: Shepler, K. (1994) Anaerobic Aquatic Metabolism of (phenyl-carbon 14)
  Thiobencarb: Lab Project Number: 397W: VP-10505.  Unpublished study prepared by
  PTRL West, Inc. 116 p.

  Patterson, T, (1994) Aerobic Metabolism of (Phenyl-(carbon 14))-Thiobencarb in Soil:
::;;:LabProject Number: PRT-08-2VNA-01;10210: PRT-p8-2VNA-01-011.  Unpublished
  sfgdy prepared by Plant Research Technologies,'Inc.  145 p.     ,
  Beiver, R. (1994) THIOBENCARB: Magnitude of the Residue in Discharged Waters and
  Receiving Waters Associated with Rice Production in Arkansas and Texas: Lab Project
  Number: 93-11-5039.  Unpublished study prepared by Valent U.S.A.  Corp. &
  Springborn Laboratories, Inc. 580 p.
Fay, D. (1994) Discussion of Factors Potentially Influencing Magnitude of the Residue
of Thiobencarb in Potable Water: Lab Project Number: 94-THI-02.  Unpublished study
prepared by Valent U.S.A. Corp.  31 p.

Ho, B. (1994) Aquatic Field Dissipation of BOLERO 10G in Rice: Lab Project Number:
T-7230. Unpublished study prepared by Chevron Chemical Co.  303 p.

Green, C. (1994) Validation of the Extraction Efficiency of Thiobencarb Residues in
                                "                     y-93-10802: RM-16D-3.
  Meat and Milk from Lactating ; goats: "Lab
  Unpublished study prepared by Valent U.S.A. Corp.  127 p.

  Ho, B. (1990) Chevron Chemical Company Phas.e 3 Reformat of MRID 00042023 and
  Related  MRIDs OQ04095Q, 00072529, 00072984, 00042026, 00042027, 00042028,
  00086773.  Residue Analysis of Bentniocarb and the Metabolites 4-Chlorobenzylmethyl
  sulfone in Rice Grain: Method RM-16A-2.  Prepared by Chevron Agchem Technical
  Center. 260 p.

  Ho, B. (1990) Chevron Chemical Company Phase 3 Reformat of MRID 00042029 and
  Related MRIDs 00042024, 00124278. Rice Grain, Rice Grain Processed Parts, Rice
  Straw, Soil, and Water: Bolero Tolerance Petition 6F1763; and Rice Weed Control,
  Bolero 8 EC, 239-EUP-77.  Prepared by Chevron Chemical Co. 45 p.
                                          144

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                   UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

                                      WASHINGTON, D.C. 20460
GENERIC DATA CALL-IN NOTICE
                                                                          OFFICE OF
                                                                     PREVENTION, PESTICIDES
                                                                     AND TOXIC SUBSTANCES
CERTIFIED MAIL
Dear Sir or Madam:                                                        .

       This Notice requires you and other registrants of pesticide products containing the
active ingredients) identified in Attachment 1 of this Notice, the Data Call-In Chemical Status
Sheet, to submit certain data as noted herein to the U.S. Environmental Protection Agency
(EPA, the Agency). These data are necessary to maintain the cpntinued registration of your
product(s) containing this active ingredient(s).  Within 90 days after you receive this Notice
you must respond as set forth in Section IE below.  Your response must state:

1.     how you will comply with the requirements set forth in this Notice and its Attachments
       1 through 4; or,

2.     why you believe you are exempt from the requirements listed in this Notice and in
      Attachment 3, Requirements Status and Registrant's Response Form, (see section DI-
       B);or,

3.     why you believe EPA should not require your submission of data in the manner
       specified by this Notice  (see section DI-D). -                                 .     -"

      If you do not respond to this Notice, or if you do not satisfy EPA that you will comply
with its requirements or should be exempt or excused from doing so, then the registration of
your product(s) subject to this Notice will be subject to suspension. We have provided a list
of all of your products subject to this Notice in Attachment 2. Data Call-in Response Form, as
well as a list of all registrants who were sent this Notice (Attachment 4).          ' -  .

      The authority for this Notice is section 3(c)(2)(B) of the Federal Insecticide, Fungicide
and Rodenticide Act as amended (FIFRA), 7 U.S.C. section 136a(c)(2)(B).  Collection of this
                                         145

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             information is authorized under the Paperwork Reduction Act by OMB Approval No.
             2070-0107 and 2070-0057 (expiration date 3-31-99).

                 This Notice is divided into six sections and five Attachments. The Notice itself contains
             information and^instructions applicable to all Data Call-in Notices. The Attachments contain
             specific chemical information and instructions. The six sections of the Notice are:
                    Section!
                    Section H
                  ^ Section m'
                    Section IV
                    Section V

                    Section VI
Why You Are Receiving This Notice
Date Required By This Notice
Compliance Wiffi'Re^uiremeiiiis Of"This'Notice
Consequences Of Failure To Comply With This Notice
Registrants' Obligation To Report Possible Unreasonable
Adverse Effects
Inquiries And Responses To This Notice
                The Attachments to this Notice are:
                    Attachment 1 -
                    Attachment 2 -
                    Attachments -
                    Attachment 4 -
Data Call-in Chemical Status Sheet
Data Call-in Response Form
Requirements Status And Registrant's Response Form
List Of All Registrants Sent This Data Call-In Notice
             SECTION I.  WHY YOU ARE RECEIVING THIS NOTICE

     	    The Agency has reviewed existing data for this active ingredient(s) and reevaluated the
             data needed to support continued registration of the subject active ingredient(s).  This
             leevaluation identified additional date necessary to assess the health and safety of the continued
             Hse of pjroducts containing this active ingredient(s). You have been sent this Notice because
   ••'	:^;:" • •: -you have iprbHuctiCs)'"contiaSing	the	subject active	ingred"ie"nt:(s).	

             SECTION H. DATA REQUIRED BY THIS NOTICE

                    A.    DATA REQUIRED

                          The date required by this Notice are specified in Attachment 3, Requirements
                    Status and Registrant's Response Form. Depending on the results of the studies
                    required in this Notice, additional testing may be required.

                    B.    SCHEDULE FOR SUBMISSION OF DATA

                                re required to submit the date of otherwise satisfy the date requirements
                            in Attachment 3, Regmremehy'Sta'tiis''^!! Registrant's Response Form.
                    within the time frames provided.
            :!	;ป,  ,        i  III	Ill  iii  I       	       ii            ,;..  "      .  . :j ,-•(!,

            *"•••:  '           I III                 I     III   I  146





[i!!! ซ: Lvii''1 p&jiiii';! •: :;!, jiiijuiiliiji"1;, .'' |N  iisl:!1!'!:1;"" '"nii','1)! j,/i!	IJlilH    if1',,,llWn!'„,:,!.'JSti'tii ill 4i,,"",''' ii1,,!,':?'!'.,;,: .V'tiiii'lllilil1^^^       ',!,,:OtAf'ip'i'vi'' .•, ,'ป•!' '''^R/iii'iliit.!'1::11111 '"'r ':, f  :,::". J1'" p   ' i. •  ,  ' • •  •':,"'.il! '''''.MI;.:,

-------
      B.     SCHEDULE FOR SUBMISSION OF DATA

             You are required to submit the data or otherwise satisfy the data requirements
      specified in Attachment 3, Requirements Status and Registrant's Response Form.
      within the time frames provided.
                                                                              (

      C.     TESTING PROTOCOL                                        ,
                studies required under this Notice must be conducted in accordance with test
      standards outlined in the Pesticide Assessment Guidelines for those studies for which
      guidelines have been established.

             These EPA Guidelines are available from the National Technical Information
      Service (NTIS), Attn: Order Desk, 5285 Port Royal Road, Springfield, Va 22161 (tel:
      703-487-4650).                                    .                       _

             Protocols approved by the Organization for Economic Cooperation and
      Development (OECD) are also acceptable if the OECD-recommended test standards
      conform to those specified in the Pesticide Data Requirements regulation (40 CFR ง
      158.70).  When using the OECD protocols, they should be modified as appropriate so
      that the data generated by the study will satisfy the requirements of 40 CFR ง 158.
      Normally, the Agency will not extend deadlines for complying with data requirements
      when the studies were not conducted in accordance with acceptable standards. The
      OECD protocols are available from 2001 L Street, N.W., Washington, D.C. 20036
      (Telephone number 202-785-6323; Fax telephone number 202-785-0350).

             All new studies and proposed protocols submitted in response to this Data Call-
      in Notice must be in accordance with Good Laboratory Practices [40 CFR Part
      160.3(a)(6)].

      D.     REGISTRANTS RECEIVING PREVIOUS SECTION 3(cY2)(E*) NOTICES
             ISSUED BY THE AGENCY      '

             Unless otherwise noted herein, this Data Call-in does not in any way supersede
      or change the requirements of any previous Data CauVInfsX or any other agreements
      entered into with the Agency pertaining to such prior Notice.  Registrants must comply
      with the requirements of all Notices to avoid issuance of a Notice of Intent to Suspend
      their affected products.                                              ,
SECTION III.       COMPLIANCE WITH REQUIREMENTS OF THIS NOTICE

      A.   , SCHEDULE FOR RESPONDING TO THE AGENCY
                                        147

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               The appropriate responses initially required by this Notice must be submitted to
        the Agency within 90 days after your receipt of this Notice. Failure to adequately
        respond to this Notice within 90 days 'of your receipt will be a basis for issuing a
        Notice of Intent to Suspend (NOIS) affecting your products. This and other bases for
        issuance of NOIS due to failure to comply with this Notice are presented in Section IV-
        A and IV-B.
              B.     OPTIONS FOR RESPONDING TO THE AGENCY •

          '•-•-The options for responding to this Notice are: 1) voluntary cancellation, 2)
        delete use(s), (3) claim generic data exemption,  (4) agree to satisfy the data
        requirements imposed by this Notice or (5) request a data waiver(s).
ii.;!
I'll;;''!'!! :
ซ:';
	IP' Ma!!
         jiiiijljiip i ' ,,ii:	I-, ;ปii' si;'., ,>i|i;,i., i n"  IAII iiK H1' y	' ' ii iiis • ' i. „.": win Jiii, EI in i1	,„ \,' i irrii"1,	 ;	' i,,,'ปK:,,;;:iu!lhi. i ;ii, „' • w,', M •' 'i	 111 in .rii, F : wi „" i 	   , i   •    •     • •••	•
         A discussion of how to respond if you chose the Voluntary Cancellation option,
    ie	Deงe:iUse(s)	option or the_ Generic	Datajxemption option is presented below. A
        s|on.of _&ง Carious ^ptions available for satisfying the data requirements of this
        ys^cpjiimned in Section ffi-C.  A discussion of options relating to requests for
  |a|a yyaiyeis iง contained in Section in-D.                    •  ...   .

         There are two  forms that accompany this Notice of which, depending upon your
:/:,;|esppnser6ne'or                                       Agency/ These forms are
  me Data-Call-in Response Form (Attachment 2) and the Requirements Status and
  Registrant's Response Form (Attachment 3). The Data Call-in Response Form must be
  submitted as part of every response to this Notice.  Please note that the company's
  authorized representative is required to sign the first page of the Data Call-In Response
  Form and Requirements	Status	and	Registrant's	Response Form (if this form is
  required) and initial any subsequent pages.  The forms  contain separate detailed
  instructions  on the response options.  Do not alter the printed material. If you have
  questions or need assistance in preparing your response, call or write the contact
  person identified in Attachment 1.

         1.     Voluntary Cancellation - You may avoid the  requirements  of this Notice
         by requesting voluntary cancellation of your product(s) containing the active
         ingredienf(s) that is the subject of this Notice.  If you wish to voluntarily cancel
         your product, you must submit a comp|ete(| pata; call-ln Response Form.
         indicating your election of this option.  Voluntary cancellation is item number 5
         on the Data Call-In Response Form. If you choose this option, this is the only
         form that you are required to complete.

                If you choose to voluntarily cancel your product, further sale and
         distribution of your product after the effective date of cancellation must be in
         accordance with the  Existing Stocks provisions of this Notice which are
         contained jfl Section IV-C.
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2.     Use Deletion - You may avoid the requirements of this Notice by
eliminating the uses of your product to which the requirements apply. If you
wish to amend your registration to delete uses, you must submit the
Requirements Status and Registrant's Response Form, a completed application
for amendment, a copy of your proposed amended labeling, and all other.
information required for processing the application. Use deletion is option
number 7 on the Requirements Status and Registrant's Response Form.  You
must also complete a Data Call-in Response Form  by signing the certification,
item number 8.  Application forms for amending registrations may be obtained
from the Registration Support and Emergency Response Branch, Registration
Division, (703)  308-8358.

       If you choose to delete the use(s) subject to this Notice or uses subject to
specific data requirements, further sale, distribution, or use of your product
after one year from the due date of your 90 day response, must bear an
amended label.

3.     Generic  Data Exemption - Under section 3(c)(2)(D) of FIFRA, an
applicant for registration of a product is exempt from the requirement to submit
or cite generic data concerning an active ingredient(s) if the active ingredient(s)
in the product is derived exclusively from purchased, registered pesticide
products containing the active ingredient(s). EPA  has concluded, as an exercise
of its discretion, that it normally will not suspend the registration of a product
which Would qualify and continue to qualify for the generic data exemption in
section 3(c)(2)(D) of FIFRA. To qualify, all of the following requirements
must be met:

       a.     The. active ingredient(s) in your registered product must be
       present solely because of incorporation of another registered product
       which contains the subject active ingredient(s) and is purchased from a
       source not connected with you; and,             ,

       b.     every registrant who is the ultimate  source of the active
       ingredient(s)  in your product subject to this DCI must be in compliance
       with the requirements of this Notice and must remain in compliance; and

       c.     you must have provided to EPA an accurate and current
       "Confidential Statement of Formula"' for  each,of your products to which
       this Notice applies.                             -

       To apply for  the Generic Data Exemption you must submit a completed
Data Call-in Response Form. Attachment 2 and all supporting documentation.
The Generic Data Exemption is item number 6a on the Data Call-in Response
Form. If you claim a generic data exemption you  are not required to complete
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       the Requirements Status and Registrant's Response Form. Generic Data
       Exemption cannot be selected as an option for product specific data.

              If you are granted a Generic Data Exemption, you rely on the efforts of
       other persons to provide the Agency with the required data.  If the registrant(s)
       who  have committed to generate and submit the required data fail to take
       appropriate steps to meet the  requirements or are no longer in compliance with
       this Data Call-In Notice, the Agency will consider that both they and you are
       not in compliance and will normally initiate proceedings to  suspend the
       registrations of both your and their product(s),  unless you commit to submit and
       do submit the required data within the specified time. In such cases the Agency
       generally will not grant a time extension for submitting the data.

       C     Satisfying the Data Requirements of this Notice -  There are various
       options available to satisfy the data requirements of this Notice., These options
       are discussed in Section ni-C of this Notice and comprise options 1 through 6
       on the Requirements Status and Registrant's Response Form and option 6b and
       7 on  the Data Call-in Response Form. If you choose option 6b or 7, you must
       submit both forms as well as any other information/data pertaining to the option
       chosen to address the data —
       5.     Request for Data Waivers. Data waivers are discussed in Section III-D
       of this Notice and are covered by options 8 and 9 on the Requirements Status
       and Registrant's Response Form.  If you choose one of these options, you must
       sjibmit both forms as well as any other information/data pertaining to the option
       chosen to address the data requirement.
                                   j,                    ,        , ,
C.     SATISFYING THE DATA REQUIREMENTS OF THIS NOTICE

       If you acknowledge on the Data. Call-In Response Form that you agree to satisfy
         gq^g^gj^" 0_e yoil ggjg^j. opgotl 65^d/ipfl7)",1'iiien you must select one of
the six options on the Requirements Status and Registrant's Response Form related to
data production for each data requirement. Your option selection should be entered
under item number 9, "Registrantr Response." The six options related to data
production are the first "six options" discussed under item]?1 in 'the instructions for
completing the Requirements Status and Registrant's Response Form.  These six
options are listed immediately below with information in parentheses to guide
registrants to additional instructions provided in this  Section.  The options are:

       1.     I will generate and submit data within the specified time frame
             (Developing Data),

       2.     I have entered into an agreement with one or more registrants to develop
             data jointly (Cost Sharing),
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3.     I have made offers to cost-share (Offers to Cost Share),

4. .    I am submitting an existing study that has not been submitted previously
       to the Agency by anyone (Submitting an Existing Study),

5.     I am submitting or citing data to upgrade a study classified by EPA as
       partially acceptable and upgradeable (Upgrading a Study),

6.     I am citing an existing  study that EPA has classified as acceptable or an
       existing study that has been submitted but not reviewed by the Agency
     ,  (Citing an Existing  Study).

Option 1. Developing Data --

       If you choose to develop the required data it must be in conformance
with Agency deadlines arid with other Agency requirements as referenced
herein and in the attachments. All data generated and submitted must comply
with the Good Laboratory Practice (GLP) rule (40 CFR Part 160), be   .
conducted according to the Pesticide Assessment Guidelines (PAG), and be in
conformance with the requirements of PR Notice 86-5. In addition, certain
studies require Agency approval of test protocols in advance of study initiation. •
Those studies for which  a protocol must be submitted have beeii identified in
the Requirements Status  and Registrant's Response Form and/or footnotes to the
form.  If you wish to use a protocol which differs from the options discussed in
Section II-C of this Notice, you must  submit a detailed description of the
proposed protocol and your reason for wishing to use it. The Agency may
choose to reject a protocol not specified in Section n-C.  If the Agency rejects
your protocol you will be notified in writing,  however, you should be aware
that rejection of a proposed protocol will not be a basis for extending the
deadline for submission  of data.          •

       A progress  report must be submitted for each study within 90 days from
the date you are required to commit to generate or undertake some other means
to address that study requirement, such as making an offer to cost-share or
agreeing to share in the cost of developing that study.  A 90-day progress report
must be submitted  for all studies. This 90-day progress report must include the
date the study was  or will.be initiated and, for studies  to be started within 12
months of commitment,  the name and address of the laboratories) or
individuals who are or will be conducting the study.

       In addition, if the time frame for submission of a final report is more
than 1 year, interim reports must be submitted at 12 month intervals from the
date you are required to commit to generate or otherwise address the
requirement for the study.  In addition to the other information specified in the
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P	!" i ,,,H" i,,"11	jl!
Ill'"If! ' 	L I :  HI ', 'i  : „ ,  UH"1!1,!"!!
 preceding paragraph, at a minimum, a brief description of current activity on
 and the status of the study must be included as well as a full description of any
 problems encountered since the last progress report.

        The time frames in the Requirements Status and Registrant's Response
 Form are the time frames that the Agency is allowing for the submission of
 completed  study reports or protocols.  The noted deadlines run from the date of
 the receipt of this Notice by the registrant.  If the data are not submitted by the
 deadline, each registrant is subject to receipt of a Notice of Intent to Suspend
 the affected registration(s).

        If you cannot submit the data/reports to the Agency in the time required
 by this Notice and intend to seek additional time to meet the requirement(s),
 you must submit a request to the Agency which includes:  (1) a detailed
 description of the expected difficulty and (2) a proposed schedule including
 alternative  dates for meeting such requirements on a step-by-step basis. You
 must explain  any technical or laboratory difficulties and provide documentation
 .from,the..laboratory performing the testing.  While EPA is considering your
 "request j"the original deadline remains.  The Agency will respond to your
 request in writing. If EPA does not grant your request, the original deadline
 remains. Normally, extensions can be requested only In cases of extraordinary
 testing problems beyond the expectation or control of the registrant. Extensions
 will not be  given in submitting the 90-day responses. Extensions will not be
 considered  if the request for extension is not made in a timely fashion; in no
 event shall  an extension request be considered if it is submitted at or after the
 lapse of the subject deadline.

 Option 2. Agreement to Share in Cost to Develop Data —

        If you choose to enter into an agreement to share in the cost of
 producing the required data but will not be submitting the data yourself, you
 must provide  the name of the registrant who will be submitting the data.  You
 must also provide EPA with documentary evidence that an agreement has been
 formed.  Such eyicjgngg...fflpy, be your letter offering to join in an agreement and
 the  other registrant's acceptance of your offer, or a written statement by the
 parties that an agreement exists. The agreement to produce the data need not
 specify all of  the terms of the final arrangement between the  parties or the
 mechanism to resolve the terms.  Section 3(c)(2)(B) provides that if the parties
. lllฐMXSlffl2?,,lte,termง,,fif	the	agreement they may resolve their  differences
 through binding arbitration.
                          Option 3. Offer to Share in the Cost of Data Development -
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       If you have made an offer to pay in an attempt to enter into an
 agreement or amend an existing agreement to meet the requirements of this
 Notice and have been unsuccessful, you may request EPA (by selecting this
 option), to exercise its discretion not to suspend your registration(s), although
 you do not comply with the data submission requirements of this Notice, EPA
 has determined that as a general policy, absent other relevant considerations, it
 will not suspend the registration of a product of a registrant who has in good
 faith sought and continues to seek to enter into a joint data .development/cost
 sharing program, but the other registrant(s) developing the data has refused to
 accept your offer. To qualify for this option, you must submit documentation
 to the Agency proving that you have made an offer to another registrant (who
 has an obligation to submit data) to share in the burden of. developing that data.
 You must also submit to the Agency a completed EPA Form 8570-32,
 Certification of Offer to Cost Share in the Development of Data. In addition,
 you must demonstrate that the other registrant, to whom the offer was made has
 not accepted your offer to enter into a cost sharing agreement by including a
 copy of your offer and proof of the other registrant's receipt of that offer (such
 as a certified mail receipt).  Your offer must, in addition to anything else, offer
 to share in the burden of producing the data upon terms to be agreed or failing
 agreement to be bound by binding arbitration as provided by FIFRA section
 3(c)(2)(B)(iii) and must  not qualify this offer.   The other registrant must also
 inform EPA of its election  of an option to develop and submit the data  required
 by this Notice by submitting a Data Call-In Response Form and a Requirements
 Status and  Registrant's Response Form committing to develop and submit the
 data required by this Notice.

      In order for you  to avoid suspension under this option, you may not
 withdraw your offer to share in the burdens of developing the data. In addition,
 the other registrant must fulfill its commitment to develop and submit the data
 as required by this Notice.  If the other registrant fails to develop the data or for
 some other reason is  subject to suspension, your registration as well as  that of ',
the other registrant will normally be subject to initiation of suspension
proceedings, unless you commit to submit, and do submit the required  data in
the specified time frame. In such cases, the Agency generally will not  grant a
time extension for submitting the data.

Option 4. Submitting an Existing Study —

      If you choose to submit an existing study in response to this Notice, .you
must determine that the study satisfies the requirements imposed by this Notice.
You may only submit a study that has not been previously submitted to  the
Agency or  previously cited by  anyone.  Existing studies are studies which
predate issuance of this Notice. Do not use this option if you are submitting
data to upgrade a study.  (See Option 5).
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       You should be aware that if the Agency determines that the study is not
 acceptable, the Agency will require you to comply with this Notice, normally
 without an extension of the required date of submission.  The Agency may
 determine at any time that a study is not valid and needs to be repeated.

       To meet the requirements of the DCI Notice for submitting an existing
 study, all of the following three criteria must be clearly met:

       a,     You must certify at the time that the existing study is submitted
       that the raw data and specimens from the study are available for audit
       and review and you must identify where they are available. This must
       be done in accdrdance with the requirements of the Good Laboratory
       Practice (GLP) regulation, 40 CFR Part 160. As stated in 40 CFR
       160.3(7) " raw data means any laboratory worksheets, records,
       memprandaj notes, or exact copies thereof, that are the result of original
       observations and activities of a study and are necessary for the
       reconstruction and evaluation of the report of that study.  In the event
       that exact transcripts of raw data have been prepared (e.g.,  tapes which
       have been transcribed verbatim, dated, and verified accurate by
       signature), the exact copy or exact transcript may be substituted for the
       original source as raw data. Raw data may include photographs,
       microfilm or microfiche copies, computer printouts, magnetic media,
       including dictated observations, and recorded data from automated
       instruments."  The term "specimens", according to 40 CFR 160.3(7),
       means "any material derived from a test system for examination or
       analysis."

       b.     Health and safety studies completed after May  1984 must also
       contain all GLP-required quality assurance and quality control
       information, pursuant to the requirements of 40 CFR Part 160.
       Registrants must also certify at the time of submitting the existing study
       that such GLP information is available for post-May 1984 studies by
    ',,//' ^ncljiding_an appropriate statement on or attached to the study signed by
m- "k ••• •" anaumorized'1 official or" representative of the registrant.
       c.     You must certify that each study fulfills the acceptance criteria
       for the Guideline relevant to the study provided in the FIFRA
       Accelerated Reregistratipn Phase;J! Technical Guidance and that the
       study has been'conducted" according'to the Pesticide Assessment
       Guidelines (PAG) or meets the purpose of the PAG (both available from
       NTIS).  A study not conducted according to the PAG may be submitted
       to the Agency for consideration if the registrant believes that the study
       clearly meets the purpose of the PAG. The registrant is referred to 40
       CFR 158.70 which states the Agency's policy regarding acceptable
                             154

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      protocols. If,you wish to submit the study, you must, in addition to
      certifying that the purposes of the PAG are met by the study, clearly
      articulate the rationale why you believe the study meets the purpose of
      the PAG, including copies of any supporting information or data. It has
      been the Agency's experience that studies completed prior to January
      1970 rarely satisfied the purpose of the PAG and that necessary raw data
      are usually not available for such studies.

             If you submit an existing study, you must certify that the study
      meets all requirements of the criteria outlined above:

             If EPA  has previously reviewed a protocol for a study you are
   *   submitting,  you must identify any action taken by the Agency on the
      protocol and must indicate, as part of your certification, the manner in
      which all Agency comments, concerns, or issues were addressed in the
      final protocol and study.
                                  i   '  i .           •           , •
             If you know of a study pertaining to any requirement in this
      Notice which does not meet the criteria outlined above but does contain
      factual information regarding unreasonable adverse effects, you must
      notify the Agency of such a study.  If such a study is in the Agency's
      files, you need only cite it along with the notification. If not in the
      Agency's files, you must  submit a summary and copies as required by
      PR Notice 86-5.                          .

Option 5. Upgrading a Study —

      If a study has been classified as partially acceptable and upgradeable,
you may submit data to upgrade that study. The Agency will review the data
submitted and determine if the requirement is satisfied.  If the .Agency decides
the requirement is not satisfied, you may still be required to submit new data
normally without any time extension.  Deficient, but upgradeable studies will
normally be classified as supplemental. However, it is important to note that
not all studies classified as supplemental are upgradeable.  If you have questions
regarding the classification  of a study or whether a study may be upgraded, call
or write  the contact person listed in Attachment  1.  If you .submit data to
upgrade  an existing study you must satisfy or supply information to correct all
deficiencies in the study identified by EPA. You must provide a clearly
articulated rationale of how the deficiencies have been remedied or corrected
and why the study  should be rated as acceptable to EPA.  Your submission must
also specify the MRID number(s) of the study which you are attempting to
upgrade  and must be in conformance with PR Notice 86-5.
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;; '•"' i ,,;ป i* JIB I
                                 Do not submit additional data for the purpose of upgrading a study
                           ฃlalsJfM ,SS,unacceptable and determined by the Agency as not capable of being
                          "upgraded.
                          Mill' , tii1:''1!:!'1" .'.rnni 'i
                                 This option should also be used to cite data that has been previously
                           submitted to upgrade a study, but has not yet been reviewed by the Agency.
                           You must provide the MRID number of the data submission as well as the
                                 The criteria for submitting an existing study, as specified in Option 4
                          above, apply to all data submissions intended to upgrade studies.  Additionally
                          yoiff ^pwssion of data intended to upgrade studies must be accompanied by a
                          certification that you comply with each of those criteria as well as a certification
                          regarding protocol compliance with Agency requirements.
                                               i^
                          Option 6. Citing Existing Studies

                                 If you choose to cite a study that has been previously submitted to EPA,
                          that study must have been previously classified by EPA as acceptable or it must
                          be a study which has not yet been reviewed by the Agency.  Acceptable
                          toncolo|vs studies generally wi|l have been .classified as ^' core-guideline" or
                          "core minimum. " For ecological effects studies, the classification generally
                          would be a rating of "core."  For all other disciplines the classification would
                          be "acceptable."  With respect to any studies for which you wish to select this
                          option you must provide the MRID number of the study you are citing and, if
                          the study has been reviewed by the Agency, you must provide the Agency ' s
                         ^.classification of the study.
                            •" ''' ' ' '                 '     1             ''                    "      '
                                       are citing a study of which you are not the original data
                               ifter, you must submit a completed copy of EPA Form 8570-31,
                          Certification with Respect to Data Compensation Requirements.

                   D.     REQUESTS FOR DATA WAIVERS

                          There are two types of data waiver responses to this Notice. The first is a
                   request for a low volume/minor; usewaiverand the second is a waiver request based on
                   your belief that the data requirement(s) are inapplicable and do not apply to your
                   product.

                          !•     Low Volume/Minor Use Waiver - Option 8 on the Requirements Status
                          and Registrant's Response Form.  Section 3(c)(2)(A) of FIFRA requires EPA to
                          consider the appropriateness of requiring data for low volume, minor use
                          pesticides. In implementing this provision EPA considers as low volume
                          pesticides only those active ingredient(s) whose total production volume for all
                                                      156

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 pesticide registrants is small. 'In determining whether to grant alow volume,
 minor use waiver the Agency will consider the extent, pattern arid volume of
 use, the economic incentive to conduct the testing, the importance of the
 pesticide, and the exposure and risk from use of the pesticide.  If an active
. ingredient(s) is used for both high volume and low volume uses, a low volume
 exemption will not be approved.  If all uses of an active ingredient(s) are low
 volume and the combined volumes for all uses are also low, then an exemption
 may be granted, depending on review of other information outlined below.  An
 exemption will not be granted if any registrant of the active ingredient(s) elects
 to conduct the testing. Any registrant receiving a low volume minor use waiver
 must remain within the sales figures in Jheir forecast supporting the waiver
 request in order to  remain qualified for such waiver. If granted a waiver, a
 registrant will be required, as a condition of the waiver, to submit annual sales
 reports. The Agency will respond to requests for waivers in writing.

       To apply for a low volume, minor use waiver, you must submit the  •
 following information, as applicable to your product(s), as part of your 90-day
 response to  this Notice:

       a.     Total company sales (pounds and dollars) of all registered  '  ••
       product(s) containing the active ingredient(s). If applicable to the active
       ingredient(s), include foreign sales for those products that are not
       registered in this country but are applied to sugar (cane or beet), coffee,
       bananas, cocoa, and other such crops. Present the above information by
       year for each of the past five years.

       b.     Provide an estimate of the sales (pounds and dollars) of the active
       ingredient(s) for each major use site.  Present the above information by
       year for each of the  past five years.

       c.     Total direct production cost of product(s) containing the active
       ingredient(s) by year for the past five years.. Include information on raw
      . material cost,  direct labor cost, advertising, sales and marketing, and
       any other significant costs listed separately.

       d.     Total indirect production cost (e.g. plant overhead, amortized
       plant and equipment) charged to product(s) containing the active
       ingredient(s) by year for the past five years. Exclude all non-recurring
       costs that were directly related to the active ingredient(s), -such as costs
       of initial registration and any data development.

       e.     A list of each data requirement for which you seek a waiver.
       Indicate the type of waiver sought and the estimated cost to you  (listed
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iliiriiiii , .'i. iJlii
               separately for each data requirement and associated test) of conducting
               the testing needed to fulfill each of these data requirements.

               f.     A list of each data requirement for which you are not seeking any
               waiver and the estimated cost to you (listed separately for each data
               requirement and associated test) of conducting the testing needed to
               fulfill each of these data requirements.

               g.     For each of the next ten years, a year-by-year forecast of
               company sales i^mdVand'dolErej1' of "the'active ingredient(s), direct
               production costs of product(s) containing the active ingredient(s)
               (following the parameters in item c above), indirect production costs of
               produces) containing the active ingredient(s) (following the parameters
               in item d above), and costs of data development pertaining to the active
               ingredient(s).
                                                         ill1 •  " • ;"'    i"    J,   ' ' • . i 'in 	 ,•ป

               h.     A description of the importance and unique benefits of the active
               ingredient(s) tousers.  Discuss the use patterns and the effectiveness of
               the active ingredient(s) relative to registered alternative chemicals, and
.,,,,..	,,,:,„	,	ME^^^&tii^&i&sfeS'	Focus	on benefits unique  to the'active"
               ingredient(s), providing information that is as quantitative as possible.
               If you do not have quantitative data upon which to base your estimates,
               then present the reasoning used to derive your estimates. To assist the
               Agency in determining the degree of importance of the active
               ingredient(s) in terms of its benefits,  you should provide information on
               any of the following factors, as applicable to your product(s):

                     (1)    documentation of the usefulness of the active ingredient(s)
               in Integrated Pest Management, (b) description of the beneficial impacts
               on the environment of use of the active ingredient(s), as opposed to its
               registered alternatives,  (c) information on the breakdown of the active
               ingredient(s) after use and on its persistence in the environment, and (d)
               description of its usefulness against a pest(s) of public health
               significance.

       Failure to submit sufficient information for the Agency to make a  determination
regarding a request for a low volume minor use waiver will result in denial of the
request for a waiver.                                        .

       2.     Request for Waiver of Data  -Option 9 on the Requirements Status and
       Registrant's Response Form,  this option may be used if you believe that a
       particular data requirement should not apply because the corresponding use is
       no longer registered or the requirement is inappropriate.  You must submit a
       rationale explaining why you believe the data requirements should not apply.
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             You must also submit the current label(s) of your product(s) and, if a current
             copy of your Confidential Statement of Formula is not already on file you must
             submit a current copy.       -

                    You will be informed of the Agency's decision in writing.  If the
             Agency determines that the data requirements of this Notice do not apply to
             your product(s), you will not be required to supply the data pursuant to section
             3(c)(2)(B), If EPA determines that the data are required for vour product^.
             you must choose a method of meeting the requirements of this Notice within the
             time frame provided fav this Notice. Within 30 days of your receipt of the
             Agency's written decision, you must submit a revised Requirements Status and
             Registrant's Response Form indicating the option chosen.
IV-    CONSEQUENCES OF FAILURE TO COMPLY WITH THIS NOTTCF

       A.    NOTICE OF INTENT TO SUSPEND

             The Agency may issue a Notice of Intent to Suspend products subject to this
       Notice due to failure by a registrant to comply with the requirements of this Data Call-
       in Notice, pursuant to FIFRA section 3(c)(2)(B).  Events which may be the basis for
       issuance of a Notice of Intent to Suspend include, but are not limited to, the following:
             1.
             2.
             3.
             4.
             5.
Failure to respond as required by this Notice within 90 days of your
receipt of this Notice.

Failure to submit on the required schedule an acceptable proposed .or
final, protocol when such is required to be submitted to the Agency for
review.   "

Failure to submit on the required schedule an adequate progress report
on a study as required by this Notice.

Failure to submit on the required schedule acceptable data as required by
this Notice.

Failure to take a required action or submit adequate information
pertaining to any option chosen to address the data requirements (e.g.,
any required action or information pertaining to submission or citation
of existing studies or offers, arrangements, or arbitration on the sharing
of costs or the formation of Task Forces, failure to comply with the
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                          Ill          III  111! Ill  II I I     I1   III I I     |H   1 ill I  I  11)11 II II 1         " . •   '  .         , 'V
                                terms of an agreement or arbitration concerning joint data development
                                or failure to comply with any terms of a data waiver).

                         6,      Failure to submit supportable certifications as to the conditions of
                                submitted studies, as required by Section ni-C of this Notice.

                         7.      Withdrawal of an offer to share in the cost of developing required data.

                         8.      Failure of the registrant to whom you have tendered an offer to share in
                                the cost of developing data and provided proof of the registrant's receipt
                                of such offer, or failure of a registrant on whom you rely for a generic
                                data exemption either to:

                                a.     inform EPA of intent to develop and submit the data required by
                                this Notice on a Data Call-in Response Form and a Requirements Status
                                and Registrant's Response Form: or,

                                b.     fulfill the commitment to develop and submit the data as required
                                by this Notice; or,
        : ',  *'i,: ';,   , ••'  ,* ..... (••• ~ซjjj ' ,-i ..... i- ..... c. ..................  otherwise take appropriate steps to meet the requirements stated
        ••". :; ill" ;.i? .' •• ซ-v ; ,*' i, ii'i ..... iซ! ( '• ^'''/(j'ji 'm^tt^'Noid.ce.i "unless ...... you" commit" 'to submit and do submit the'required
'' .....................     ........... " .......... '''   1"""' ......... "" ....... 'data" in the specified time frame.

                         9.     Failure to take any required or appropriate steps, not mentioned above,
                         at any time following the issuance of this Notice.
n Fi! ! PHI ','",!': ....... !,,  !' .'  H ...... |,i!,| ' '!':• "'!'„„'  , , ',i,'ป ' * ......... '. ' ' 'P1 Pllll! ' 'll'i'li'ii'l ' I I'll I1  I ....... Ill I I ...... ill .......... Htm ill. 'ii ''• '11 .......... I T ' ' 'P  II ' "I1 ''' ..... I Till"! l 1 ||f |i| ' i '  |" 'i||||||i|| "||i" 'I .......... I ' • ,,i " '  "  .   •   .'  i
........    i

                  B.     BASTS FOR DETERyI^^IQ^THAT SUBMITTED STtJDY IS
                         UNACCEPTABLE

                         The Agency may determine that a study (even if submitted within the required
                 • time) is unacceptable  and constitutes a basis for issuance of a Notice of Intent  to
                  Suspend.  The grounds for suspension include, but are not limited to, failure to meet
                  any of the following :

                         1.     EPA requirements specified in the Data Call-In Notice or other
                         documents incorporated by reference (including, as applicable, EPA Pesticide
                         Assessment Guidelines, Data Reporting Guidelines, and GeneTox Health
                         Effects Test Guidelines) regarding the design, conduct, and reporting of
                         required studies.  Such requirements  include, but are not limited to, those
 ,™ ;'.,•;"   •  ;:;"::;:           relating to test material, test procedures, selec^^                         '•
                         animals, sex and distnbSition "of' 'animals^ 36se arid effect levels to be tested or
                         attained, duration of test, and, as applicable, Good Laboratory Practices.
                                                    .''KiM^R ."• „ ,•,"ซ,;':

-------
 c.
2.     EPA requirements regarding the submission of protocols, including the
incorporation of any changes required by .the Agency following review.

3.     EPA requirements regarding the reporting of data, including the manner
,of reporting, the completeness of results, and the adequacy of any required
supporting (or raw) data, including, but not limited to, requirements referenced
or included in this Notice or contained in PR 86-5.  All studies must be
submitted in the form, of a final report; a preliminary report will not be
considered to fulfill the submission requirement.

EXISTING STOCKS OF SUSPENDED OR CANCELLED PRODUCTS
       EPA has statutory authority to permit continued sale, distribution and use of
 existing stocks of a pesticide product which has been suspended or cancelled if doing so
 would be consistent with the purposes of the Federal Insecticide, Fungicide, and
 Rodenticide Act.

       The Agency has determined that such disposition by registrants -of existing
 stocks for a suspended registration when a section 3(c)(2)(B) data request is outstanding
 would generally not be consistent with the Act's purposes.  Accordingly, the Agency
 anticipates granting registrants permission to sell, distribute, or use existing stocks of
 suspended product(s)  only in exceptional circumstances. If you believe such.
 disposition  of existing stocks of your product(s) which may be suspended for failure to
 comply with this Notice should be permitted, you have the burden of clearly
. demonstrating to EPA that granting such permission would be consistent with the Act.
 You must also explain why an "existing stocks" provision is necessary, including a
 statement of the quantity of existing stocks and your estimate of the time required for
 their sale, distribution, and use. Unless you meet this burden the Agency will not
 consider any request pertaining to the continued sale, distribution, or use of your -
 existing stocks after suspension.
                                                         w
    .  If, you  request a voluntary cancellation of your product(s) as a, response to this
 Notice and  your product is in full compliance with all Agency requirements, you will
 have, under most circumstances, one year from the date your 90 day response to this
 Notice is  due, to sell, distribute, or use existing stocks.  Normally, the Agency will
 allow persons other than the registrant such as independent distributors, retailers and
 end users to sell, distribute or use such existing stocks until the stocks are exhausted.
 Any sale, distribution or use of stocks of voluntarily cancelled products containing an
 active ingredient(s) for which the Agency has particular risk concerns will be
 determined on case-by-case basis.               •

       Requests for voluntary cancellation received after the 90 day response period
 required by this Notice will not result in the Agency granting any additional time to
 sell, distribute, or use existing stocks beyond a year from the date the 90 day response
                                    161

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       was due unless you demonstrate to the Agency that you are in full compliance with all
       Agency requirements, including the requirements of this Notice.  For example, if you
       decide to voluntarily cancel your registration six months before a 3 year study is
       Schedulgd to be submitted, all progress reports and other information necessary to
       establish that you have been conducting the study in an acceptable and good faith
       manner must have been submitted to the Agency, before EPA will consider granting an
       existing stocks provision.
SECTION V. REGISTRANTS' OBLIGATION TO REPORT POSSIBLE UNREASONABLE
             ADVERSE EFFECTS

       Registrants are reminded that FIFRA section 6(a)(2) states that if at any time after a
pesticide is registered a registrant has additional factual information regarding unreasonable
adverse effects on the environment by the pesticide, the registrant shall submit the information
to the Agency. Registrants must notify the Agency of any factual information they have, from
whatever source, including but not limited to interim or preliminary results of studies,
regarding unreasonable adverse effects on man or,the environment. This requirement
continues as long as the products are registered by the Agency.
SECTION VI.
INQUIRIES AND RESPONSES TO THIS NOTICE
       If you have any questions regarding the requirements and procedures established by
this Notice, call the contact person listed in Attachment 1, the Data Call-in Chemical Status
                                  '                       '
       All responses to this Notice (other than voluntary cancellation requests and generic data
exemption claims) must include a completed natarCall-][n Response Form (Attachment 2) and
a complete3 Requirements Status and Registrant' s Response Form (Attachment 3) and any
other documents required by this Notice, and should be submitted to the contact person
identified in Attachment 1. If the voluntary cancellation or generic data exemption option is
chosen, only the Data Call-in Response Form need be submitted.
      The Office of Compliance (OC) of the Office of Enforcement and Compliance
Assurance (OECA), EPA, will be monitoring the data being generated in response to this
Notice.
                                             Sincerely yours,
                                             Lois A. Rossi, Director
                                             Special Review and
                                               Reregistration Division
                                        162

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2665 DATA CALL-IN CHEMICAL STATUS SHEET
                                                           i
INTRODUCTION      ,                       .

       You have been sent this Generic Data Call-in Notice because you have product(s) .
containing thiobencarb.

       This Generic Data Call-in Chemical Status Sheet, contains an overview of data
required by this notice, and point of contact'for inquiries pertaining to the reregistratiqn of
thiobencarb. This attachment is to be used in conjunction with (1) the Generic Data Call-in
Notice, (2) the Generic Data Call-In Response Form (Attachment 2), (3) the Requirements
Status and Registrant's Form (Attachment 2), (4) a list of registrants receiving this DCI
(Attachment 4), (5) the EPA Acceptance Criteria (Attachment 5), and (6) the Cost Share and
Data Compensation Forms in replying to this thiobencarb Generic Data Call In (Attachment
F). Instructions and guidance accompany each form.

DATA REQUIRED BY  THIS NOTICE
       The additional data requirements needed to complete the generic database for 2665 are
contained in the Requirements Status and Registrant's  Response. Attachment C.  The Agency
has concluded that additional product chemistry data on thiobencarb are needed.  These data
are needed to fully complete the reregistration of all eligible thiobencarb products.

INQUIRIES AND RESPONSES TO THIS NOTICE

       If you have any questions regarding the generic data requirements and procedures
established by this Notice, please contact Patrick Dobak at (703) 308-8180.   .

       All respohsades to this Notice for the generic data requirements should be submitted to:

             Patrick Dobak, Chemical Review Manager
             Reregistration Branch I
             Special Review and Registration Division (H7508W)
             Office of"Pesticiafde Programs
             U.S. Environmental Protection Agency
             Washington, D.C.  20460
             RE: Thiobencarb
                                         163

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                                                                            164
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-------
SPECIFIC INSTRUCTIONS FOR THE GENERIC DATA CALL-IN RESPONSE FORM

       This Form is designed to be used to respond to call-ins for generic and product specific
data for the purpose of reregistering pesticides under the Federal Insecticide Fungicide and
Rodenticide Act. Fill out this form each time you are responding to a data call-in for which EPA
has sent you the form entitled "Requirements Status and Registrant's Response."

       Items  1-4 will have been preprinted on the form Items 5 through 7 must be completed by
       the registrant as appropriate Items 8 through 11 must be completed by the registrant before
       submitting a response to the Agency.        '  '   . .                              ,

       Public reporting burden for this collection of information is estimated to average 15
minutes per response, including time for reviewing instructions, searching existing data sources,
gathering and maintaining the data needed, and completing and reviewing the collection of
information.  Send comments regarding the burden estimate or any other aspect of this collection
of information, including suggesting for reducing  this burden,  to Chief, Information Policy
Branch, PM-223, U S Environmental Protection Agency, 401 M St, S W , Washington, D C
20460; and to the Office of Management and Budget, Paperwork Reduction Project 2070-0107
Washington,'DC 20503.                                                              '

INSTRUCTIONS
      Item L

      Item 2.


      ItemS.

      Item 4.
      Item 5.
This item identifies your company name, number and address.

This item identifies the ease number, ease name, EPA chemical number
and chemical name.

This item identifies the date and type of data call-in.

This item identifies the EPA product registrations relevant to the data
call-in. Please note that you are also responsible for informing the Agency
of your response regarding any product that you believe may be covered
by this data call-in but .that is not listed by the Agency in Item 4. You must
bring any such apparent omission to the Agency's attention within the
period required for submission of this response form.

Cheek this  item  for each product registration  you  wish to  cancel
voluntarily. If a registration number is listed for a product for which you
previously requested voluntary cancellation, indicate in Item 5 the date of.
that request.  You do not need to complete any item on the Requirements
Status and Registrant's Response Form for any product that is voluntarily
cancelled.
                                         167

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Item 6a.
 Item 6b.
Check this item if this data call-in is for generic data as indicated in Item
3 and if you are eligible for a Generic Data Exemption for the chemical
listed in Item" 2 and used in the subject product.    By electing this
exemption, you  agree to  the terms  and conditions of  a Generic Data
Exemption as explained in the Data Call-in Notice.

If you are eligible for or claim a Generic Data Exemption, enter the EPA
registration Number of each registered source of that active ingredient that
you use in your product.

Typically, if you purchase an EPA-registered product from one or more
other producers  (who, with respect to the incorporated product, are in
compliance with this and-any other outstanding Data Call-in Notice), and
incorporate that product into all your products, you may complete this item
for all products  listed on this form If, however, you produce the active
j^g^g-ฃt yo^ggj^Oj use ฃ^y"unregistered" product (regardless of the fact
that some of your sources  are registered), you may not claim a Generic
Data Exemption and you may not select this item.

Check this Item  if the .data call-in is a generic data call-in as indicated in
'jftem'3' and	ff'ybu	are" agreeing to'satisfy	the "generic  data requirements"of
this  data call-in.    Attach  the Requirements Status and  Registrant's
Response Form that indicates how you will satisfy those requirements.
 Item 7a.      Check this item if this call-in if a data call-in as indicated in Item 3 for a
              manufacturing use product (MUP), and if your product is a manufacturing
              use product for which you agree to supply product-specific data. Attach
              the Requirements Status and Registrants' Response Form that indicates how
              you will satisfy those requirements:

 Item 7b.      Check this item if this call-in is a data call-in for an end use product (EUP)
              as indicated in Item 3 and if your product is an  end use product for which
              you agree to supply product-specific data.  Attach the Requirements Status
              and Registrant's Response Form that indicates  how you will satisfy those
"' •  ;"'"' '' •	•'	' ' "'"" " '"requirements.

 Item 8.       This certification statement must be signed by an authorized representative
              of your company and the person signing must  include  his/her title.
              Additional pages used in your response must be initialled and dated in the
              space provided for the certification.

 Item 9.       Enter the date of signature.

 Item 10.      Enter the name of the person EPA should contact with questions regarding
 	•  ":;::	: ':   your response	"	
 Item 11.      Enter the phone number of your company contact.
                                    168

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                                 170

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 SPECIFIC INSTRUCTIONS FOR COMPLETING THE REQUIREMENTS STATUS AND
 REGISTRANTS RESPONSE FORM
                                        1          '                        '        '
 Generic Data

 This  form is  designed to be used for. registrants to  respond to call-in-  for generic and
 product-specific data as part of EPA's reregistration program under the Federal Insecticide
 Fungicide and Rodenticide Act.  Although the form is the same for both product specific and
 generic data, instructions  for completing the forms differ slightly.  Specifically, options for
 satisfying product specific data requirements do not include (1) deletion of uses or (2) request for
 a  low volume/minor use waiver.   These  instructions  are  for completion of generic data
 requirements.      '                           ;

 EPA has developed this form individually for each data call-in addressed to each registrant, and
 has preprinted this form with a number of items.  DO NOT use this form for any other active
 ingredient.            .                                                  .

 Items 1 through 8 (inclusive) will have been preprinted on the form. You must complete all other
 items on this form by typing or printing legibly.  •-                 .

 Public reporting burden for .this collection of information is estimated to average 30 minutes per
 response, including time for reviewing instructions, searching existing data sources, gathering and
 maintaining the data needed, and completing and reviewing the collection of information. Send
 comments regarding  the burden estimate or  any other aspect of, this collection of information,
 including suggesting  for reducing this burden, to Chief,  Information Policy Branch, PM-223'
 U.S. Environmental Protection Agency, 401  M St., S.W., Washington, D.C. 20460; and to the
 Office of Management and Budget, Paperwork Reduction Project 2070-0107, Washington D C
 20503.  •                                                                       '   '  '

 INSTRUCTIONS

 Item 1.       This item identifies your company name, number, and address.

 Item 2.
Item3.

Item 4.



Item 5.
This item identifies the case number, case name, EPA chemical number and
chemical name.

This item identifies the date arid type of data call-in.
          *                             '
This item identifies the guideline reference numbers of studies required to support
the product(s) being reregistered. These guidelines,  in addition to requirements
specified in the Data Call-In Notice, govern the conduct of the required studies.

This item identifies the study title associated with the  guideline reference number
and whether protocols and 1, 2, or 3-year progress reports are required to be
                                         171

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                          submitted in connection withthe study.  As noted in Section HI of the Data Call-In
                          Notice, 90-day progress reports are required for all studies.
                                If an asterisk appears in Item 5, EPA has attached information relevant to
                                this guideline J.^QJ.Q^ ^^^ to-the gg^^gme]nts gt^s and Registrant's
                                Response Form.
             Item 6.
  $::.'!?•
 This item identifies the code associated with the use pattern of the pesticide. A
 br,ief description of each code follows:
;ill  j;>:''";"'ii&ftft'IS vm	it ISi"	IllKMSWiftR,	*•:ll':; lii'.:I SIM i:- :•'.?''.;. ,  'I  ' ,, •.. • .     : ••"'•• • -:
                                A.
                                B.
                                C.
                                D.
                                E.
                                F.
                                G.
                                H.
                                I.
                                J.
                                K.
                                L.
                                M.
                                N,
                                O.
                           Terrestrial food
                         	Terrestrial, feed	
                           Terrestrial non-food
                           Aquatic food
                           Aquatic non-food outdoor
                         |||, j|" y.iji, |,i T.	 , f,	i	„,	| ,, f	, 	  r, ,
                         M'"t' iiir Aquatic'non-food industrial
                           Aquatic non-food residential
                           Greenhouse food
                           Greenhouse non-food crop
                         	Forestry	
                           Residential
                           Indoor food
                           Indoor non-food
                           Indoor medical
                           Indoor residential
             Item?.
 This item identifies the ,cpde assigned to the substance that must be used for
 testing. A brief description of each code follows.
       EP
                                                    End-Use Product
       MP/TGAI

       PAI
       PAI/M
       PAI/PAIRA

       PAIRA
       PAIRA/M
       PAIRA/PM

       TEP
       TEP   *
                                                    Manufacturing-Use Product and Technical Grade
                                                    Active Ingredient
                                                    Pure Active Ingredient
                                                    Pure Active Ingredient and Metabolites
                                                    Pure Active Ingredient or Pure Active Ingredient
                                                    Radiolabelled
                                                    Pure Active Ingredient Radiolabelled
                                                    Pure Active Ingredient Radiolabelled and Metabolites
                                                    Pure Active Ingredient Radiolabelled and Plant
                                                    Metabolites
                                                    Typical End-Use Product
                                                    Typical End-Use Product, Percent Active Ingredient
                                                    Specified ,
                                                      172
liiiiiin^^    	i

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              TEP/MET
.              TEP/PAI/M

              TGAI/PAIRA

              TGAI
              TGAI/TEP

              TGAI/PAI

            -  MET
              IMP
           ;   DEGR

*See: guideline comment
                                        Typical End-Use Product and Metabolites
                                      .  Typical End-Use Product or. Pure Active Ingredient
                                        and Metabolites
                                        Technical Grade Active Ingredient or Pure Active
                                        Ingredient Radiolabelled
                                        Technical Grade Active Ingredient
                                        Technical Grade Active  Ingredient or Typical
                                        End-Use Product
                                        Technical Grade Active Ingredient or Pure Active
                                        Ingredient
                                        Metabolites
                                        Impurities
                                        Degradates
Item 8.
Item 9.
       This item identifies the time frame allowed for submission of the study or protocol
       identified in item 2. The time frame runs from the date of your receipt of the Data
       Call-in Notice.     '                            •.               .
                                                 \
       Enter the appropriate Response Code or. Codes to show how you intend to comply
       with each data requirement. Brief descriptions of each code follow. The Data Call-
       in Notice contains a fuller description of each of these options.
       1.
       2.
       3.
                    (Developing Data) I will conduct a new study and submit it within the time
                    frames specified in item 8 above.  By indicating that I have chosen this
                    option, I certify that I will comply with all the requirements pertaining to
                    the conditions for submittal of this study as outlined in the Data Call-In
                    Notice and that I will provide the protocol and progress reports required in
                    item 5 above.

                    (Agreement to Cost Share) I have entered into an agreement with one or
                    more registrants to develop data jointly. By indicating that I have chosen
                    this option, I certify that I will comply with all the requirements pertaining
                    to sharing in the cost of developing  data as outlined in the Data CalHn
                    Notice.

                    (Offer to Cost Share) I have made an offer to enter into an agreement with
                    one or more registrants to develop data jointly. I am submitting a copy of
                    the form "Certification of Offer to Cost Share in the Development of Data"
                    that describes this offer/agreement'.  By indicating that I have chosen this
                    option, I certify that I will comply with all the requirements pertaining to
                    making an offer to share in the cost of developing data as outlined in the
                    Data Call-In Notice.
                                  173

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                          4.
                          	1:11 M.
                          K'M .•>.'.
                          5.
                           	Ill
                          7.



                          8.
                          in in
                           III nil
1 'i'l' I1,.;,'!!!!,,1'1 iซ!!ii, T"
      (Submitting Existing Data) I am submitting an existing study that has never
      before been submitted to EPA. By indicating that I have chosen this option,
      I certify that this study meets all the requirements pertaining  to  the
      cdhoitions for submittal of existing data outlined in the Data Call-in Notice
      ,j', Ijpivil^  u'lil'l".!!!!!!":11" '^1 shil'''Pi,!' ''I1 ™! i* !i ill I'll: IIJ,1" HI ..'I,'"1!"!,"!":,!!	'"SI I'll'ITS!?!	SSi'i-hl!	HIT ,	,	,i ,	i"	"ป':'•	'  •• t "' , , , ' ซ ' '.   ^ • ,     *''l ' ' 1" •'
      and I have attached the needed supporting information  along with this
      response.
      (Upgrading a Study) I am submitting or citing data to upgrade a study that
      EPA has classified as partially acceptable and potentially upgradeable. By
      indicating that I have chosen this option, I certify that I have met all the
      requirements pertaining to the conditions for submitting or citing existing
      data to  upgrade a study described in  the Data Call-in Notice.  I  am
      indicating on attached correspondence the Master Record Identification
      Number (MRID) that EPA has assigned to the data that I am citing as well
      as the MRID of the study I am attempting to upgrade.
                                 (Citing a Study) I am citing an existing  study that has been previously
                                 classified by EPA as acceptable, core, core minimum, or a study that has
                                 not yet been reviewed by the Agency.  I  am providing  the Agency's
                                 classification of the study.
       (Deleting  Uses) I am attaching an application  for amendment to my
       registration deleting the uses for which the data are required.

       (Low Volume/Minor  Use Waiver Request)  I have read the statements
       concerning low volume-minor use data waivers in the Data Call-in Notice
       and I request a low-volume 'minbf'use'waiver of the data requirement.  I
       am  attaching  a detailed justification  to  support this  waiver request
       including, among other things, all information required to support the
       request. I understand that, unless' modified by the Agency in writing, the
       data requirement as stated in the Notice governs.
             Item 10.
9.     (Request for" Waiver of Data) I'have"read the statements concerning data
       waivers other than low volume minor-use data waivers in the Data Call-In
       Notice and I request a waiver of the data requirement. I am attaching an
       identification of the basis for this waiver and a detailed justification to
       support this waiver request. The justification includes, among other things,
       all information required to support the request. I understand that, unless
       modified by the Agency in writing, the data requirement as stated in the
       Notice governs.

This item must be signed by an authorized representative of your company. The
person signing mustincmde his/her title, and must initial and date all other pages
of this form.
                                                       174

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Item 11.

Item 12.
Enter the date of signature.


Enter the name of the person EPA should contact with questions regarding your
response.                      ,                                      '  .
Item 13.    •  Enter the phone number of your company contact.
                                        175

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                                                                                                                           'S1: KM!
                                                                    176

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                                                                         178

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          \
                    UNITED STATES ENVIRONMENTAL PROTECTION AGENCY
              WASHINGTON, D.C  20460
                                                                             OFFICE OF
                                                                        PREVENTION, PESTICIDES
                                                                        AND TOXIC SUBSTANCES
                               DATA CALL-IN NOTTCP.
 CERTIFIED MAIL
Dear Sir or Madam:
This Notice requires you and other registrants of pesticide products containing the active
ingredient identified in Attachment 1 of this Notice, the Data Call-in Chemical Status Sheet, to
.submit certain product specific data as noted herein to the U.S. Environmental Protection
Agency (EPA, the Agency).  These data are necessary to  maintain the continued registration of
your product(s) containing this active ingredient.  Within 90 days after you receive this Notice
you must respond as set forth in Section ffl below. Your response must state:

       1.      How you will comply with the requirements set forth in this Notice and its    '
              Attachments 1 through 6; or

              Why you believe you are exempt from the  requirements listed in this Notice and
              in Attachment 3, Requirements Status and Registrant's Response Form, (see
              section  ni-B); or

              Why you believe EPA should not require your submission  of product specific
              data in the manner specified by this Notice (see section lE-D).

       If you do n9t respond to this Notice,  or if you do not satisfy EPA that you will comply
with its requirements or should be exempt or excused from doing so, then the registration of
2.
3.
                                          179

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 your product(s) subject to this Notice will be subject to suspension. We have provided a list of
 all of your products subject to this Notice in Attachment 2, Data Call-in Response Form, as
     as a list of all registrants who were sent this Notice (Attachment 6).
       The ; authority for this Notice is section 3(c)(2)(B) of the Federal Insecticide, Fungicide
 and Rodenticide Act as amended (FIFRA), 7 U.S.C. section 136a(c)(2)(B).  Collection of this
 Information is authorized under the Paperwork Reduction Act by 6MB Approval No. 2070-
 0107 and 2070:0057 (expkation date 03-31-99).

       This Notice is divided into six sections and six Attachments.  The Notice itself contains
 infonnation and instructions applicable to all Data Call-In Notices.  The Attachments contain
 specific chemical information and instructions. The six sections of the Notice are:

       Section I  -  Why You Are Receiving This Notice
       Section II  -   Data Required By This Notice
       Section IE -   Compliance With R^guirem^ite Of This Notice
ii'T..  i'i^teS^^^^                                                   ......... '..   '  '.
       Section V  -   Registrants' Obligation To Report Possible Unreasonable Adverse
 , ....................... .................... ........ Effects [[[ , ............................................ . ...................... .. ; ........
       Section VI -   Inquiries And Responses To This Notice

 The Attachments to  this Notice are:

       1 -    Data  Call-in Chemical Status Sheet
       2 -    Product-Specific Data Call-in Response Form
       3 -    Requirements Status and Registrant's Response Form
       4 -    EPA  Batching of End-Use Products for Meeting  Acute Toxicology Data
              Requirements for Reregistration
       5 - ..... 7  List of Registrants Receiving This Notice
       6 -    Cost  Share and Data Compensation  Forms and the Confidential Statement of
              Formula Form
 SECTION!  WHY YOU ARE RECEIVING THIS NOTICE

       The Agency has reviewed existing data for this active ingredient arid reevaluated the
 data needed to support continued registeation' of the subject" active ingredient.  The' Agency has
 concluded that the only additional data necessary  are product specific data.  No additional
 generic data requirements are being imposed.  You have been sent this Notice because you
 have product(s) containing the subject active ingredient.


-------
       The product specific data required by this Notice are specified in Attachment 3,
Requirements Status and Registrant's Response Form.  Depending on the results of the. studies
required in this Notice, additional testing may be required.    t

H-B. SCHEDULE FOR SUBMISSION OF DATA

   You are required to submit the data or otherwise satisfy the data requirements specified in
Attachment 3, Requirements Status and Registrant's Response Form, within the time frames
provided.

H-G. TESTING PROTOCOL             '

   All studies required under this Notice must be conducted in accordance with test  standards
outlined in the Pesticide Assessment Guidelines for those studies for which guidelines have
been established.

       These EPA Guidelines are available from the National Technical Information Service
(NTIS), Attn: Order Desk, 5285 Port Royal Road, Springfield, Va 22161 (tel: 703-487-4650).

       Protocols approved by the Organization for Economic Cooperation and Development
(OECD) are  also acceptable if the OECD-recommended test standards conform to those
specified in the Pesticide Data Requirements regulation (40 CFR ง 158.70).  When using the
OECD protocols, they should be modified as appropriate so that the data generated by the
study will  satisfy the requirements of 40 CFR ง 158. Normally, the Agency will not extend
deadlines for complying with data requirements when the studies were not conducted in
accordance with acceptable standards.  The OECD protocols are available from OECD, 2001  L
Street,  N.W., Washington, D.C. 20036 (Telephone number 202-785-6323; Fax telephone
number 202-785-0350).

       All new studies and proposed protocols submitted in response to this Data Call-in
Notice must be in  accordance with Good Laboratory Practices [40 CFR Part 160.3(a)(6)].

n-D. REGISTRANTS RECEIVING PREVIOUS SECTION 3(cY2)CB-) NOTICES
     ISSUED BY THE AGENCY

     •Unless otherwise noted  herein, this Data Call-In does not in any way supersede or change
the requirements of any previous Data Call-In(s). or any other agreements entered into with the
Agency pertaining to such prior Notice. Registrants must comply with the requirements of all
Notices to  avoid issuance of  a Notice of Intent to,Suspend their affected products.

SECTION ffl.  COMPLIANCE WITH REQUIREMENTS OF THIS NOTICE

ffl-A.  SCHEDULE FOR RESPONDING TO THE AGENCY

       The appropriate responses initially required by this  Notice for product specific data
must be submitted to the Agency within 90 days after your receipt of this-Notice. Failure to
adequately respond to this Notice within 90 days  of your receipt will be a basis for issuing a

                                        .181

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I:'!:',.!'' I'd1; "K'i'S11"
   	,1;":::
   : ,',|i,,r,, "ill! i,"*
   ,;,,["'I	,,|l| ,,„!'
ill!"]!1"!" i :	hiii'ti ,'is::"!

 ,'""" I hlljll'l,'!,' I''SIP III,"
  i :! "I11; 111,! 1,1 j! 'II,

fill", I ,"lli, :,,"'in
ii hi: ""Hi'
ft*
             Notice of Intent to Suspend (NOIS) affecting your products. This and other bases for issuance
             of NOIS due to failure to comply with this Notice are presented in Section IV-A and IV-B.

             m-B. OPTIONS FOR RBSPONblNG TO THE AGENCY

                    The options for responding to this Notice for product specific data are: (a) voluntary
             cancellation, (b)  agree to satisfy the product specific data requirements imposed by this notice
             or (c) request a data waiver(s).
                    A discussion of how to respond if you chose the Voluntary Cancellation option is
             presented below.  A discussion of the various options available for satisfying the product
             specific data requirements of this Notice is contained in Section ni-C.  A discussion of options
             relating to requests for data waivers is contained in Section jjf.j)

                    There are two forms that accompany this Notice of which, depending upon your
             response, one or both must be used in your response to the Agency.  These forms are the Data-
             Call-in Response Form,  and the Requirements Status and Registrant's Response Form.
             Attachment 2 and Attachment 3. The Data Call-in Response Form must be submitted as part of
             every response to this Notice. In addition, one copy of the Requirements Status and
             Registrants Response Form  must be submitted for each product listed on the Data Call-in
             Response Form unless the voluntary cancellation option is selected or unless the product is
             identical to another (refer to  the instructions for completing the Data Call-In Response Form in
             Attachment 2).  Please note that the company's authorized representative is required to sign the
             first page of the Data Call-In Response Form and Requirements Status and  Registrant's
             Response Form (if this form is required) and initial any subsequent pages. The forms contain
           .;  '^ปa|eletailed	instructions	on the response options.  Dp not alter the printed material.  If
             you Have questions or need assistance in preparing your response, call or write the contact
             J	"-"""	"s *"""• -^	\	m'Attachment	l".	
                   1,1.1!	li, I,, I"1:::	' nil, „!
                              jiiVlU""!' ill. "I! II	I hf 'ป' ''ill, |,|!||
                                            	i!'i, iii	ijii.1'i. (i: <:;:,,'i iKWiiii'h'i
                Xlli!	Kiln i! ,,i!,| IK I,:1',
                             in n	 n |. i nil"!" jTiiiii • 'iii,'iiii'iii,; [uiinii.':;!1!::!,:,,,,!!!!!1 ^ .? m^,'. IF	in	iiiii'iS'iiiiiiiHiiiii',1:',!!'!;"!!,'!',!,,,, ii"iP!i: j-BNiiiiJ!'1'' \v Misi/h ni'iiniiif1!'1",!*!!!1!1!!!'! "iUhJiiiiiiii; :i:"'iiii
                    1 . Voluntary Cancellation - You may avoid the requirements of this Notice by
             requesting voluntary cancellation of your product(s) containing the active ingredient that is the
             libject of this Notice. If you wish to voluntarily cancel your product, you must submit a
             (!omj^leted Data Call-In Response Form, indicating your election of this option.  Voluntary
             q                 numbef ฃ- --- ^ ]pata Cali-5i Response ponฃ "if you choose this option,
             this is the only form that you are required to complete.

                   |f you chose to voluntarily cancel your product, further sale and distribution of your
             product after the effective date of cancellation must be in accordance with the Existing Stocks
             provisions of this Notice which are contained in Section IV-C.
             iiiiiiLi'i i • i>< ,1',' '''.''r '     iillilll  i    I '    I  ii mi 1 1  i  ll ill i mi nil I i i  i  'I liiii I 'I i ill   i  1111111 I             .  ... ':' "'       ...  ,- "

                   2v Satisfying the Product Specific Data Requirements of this Notice There are various
             options available to satisfy the product specific data requirements of this Notice.. These options
             ^^is^^ui.^iti.o^jnt.Q ...... of .this ....... Notice,,, and ..... comprise options 1 through 6 on the
             RejpkemeMsJStatus and Registrant's Response Form and item numbers 7a and 7b  on the Data
             Call-in Response Form. Deletion of a use(s) and the low volume/minor use option  are not valid
             options for fulfilling product specific  data requirements.
liiii ....... ;!" i, aii'i:
                       . v ..... mil
                                                       182
If;;;1 i,,,	

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        3. Request for Product Specific Data Wflivars.  Waivers for product specific data are '
 discussed in Section IH-D of this Notice and are covered by option 7 on the Requirements
 Status and Registrant's Response Form. If you choose one of these options, you must submit
 both forms as well as any other information/data pertaining to the option chosen to address the
 data requirement.        ,

 m-C  SATISFYING THE DATA REQUIREMENTS OF THIS NOTTCF

        If you acknowledge on the Data Call-in Response Form that you agree to satisfy the
 product specific data requirements (i.e. you select item number 7a or'7b), then you must select
 one of the six options on the Requirements Status and Registrant's Response Form related to
 data production for each data requirement.  Your option selection should be entered under item
 number 9, "Registrant Response."  The six options related to  data production are the first six
 options discussed under item 9 in the instructions for completing the Requirements Status and
 Registrant's Response Form. These six options are listed immediately below with information
 in parentheses to guide registrants to additional instructions provided in this Section. The
 options are:                 ,                                        ,        '

        (1)     I will generate and submit data within the specified time frame (Developing
              Data)       •
        (2)     I have entered into an agreement with one  or more registrants to develop data
              jointly (Cost Sharing)
        (3)     I have made offers to cost-share (Offers to  Cost Share)
       (4)     I am submitting an existing study that has not been submitted previously to the
              Agency by anyone (Submitting an Existing Study)
       (5) ,    I am submitting or citing data to upgrade a study classified by EPA as partially
              acceptable and upgradeable (Upgrading a Study)
      • (6)     I am citing an existing study that EPA has classified, as acceptable or an existing
              study that has been submitted but not reviewed  by  the Agency (Citing an
              Existing Study)

       Option 1,  Developing Data - If you choose to develop the required data it must be in
 confprmance with Agency deadlines and with-other Agency requirements as referenced herein
 and in the attachments. All data generated and submitted must comply with the Good
 Laboratory Practice (GLP) rule (40 CFR Part 160), be conducted according to the Pesticide
 Assessment  Guidelines (PAG), and be in conformance with the requirements of PR Notice 86-


    •   The time frames in the Requirements Status and Registrant's Response Form are the
 time frames  that the Agency is allowing for the submission of completed study reports.  The
 noted deadlines run from the date of the receipt of this Notice by the registrant.  If the data are
•not submitted by the deadline, each registrant is subject to receipt of a Notice of Intent to
 Suspend the affected registration(s).

       If you cannot submit the data/reports to the Agency in the  time required by this Notice
 and intend to seek additional time to meet the requirements (s), you must submit a request to the
 Agency which includes:  (1) a detailed description of the expected difficulty and (2) a proposed

                   '   .       '   .  .  •    183

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 schedule including alternative dates for meeting such requirements on a step-by-step basis.
 You 'must explain 'any| techrucal'oflaboratory difficulties and provide documentation from the
 laboratory"peHormuig'tHe testing.  While T3? A is considering your request, the original
 deadline remains. The Agency will respond to your request in writing. If EPA does not grant
 your request, the original deadline remains.  Normally, extensions can be requested only in
 cases of extraordinary testing problems beyond the expectation or control of the registrant.
 Extensions will not be given in submitting the 90-day responses.  Extensions will not be
 considered if the request for extension is not made in a timely fashion; in no event shall an
 extension request be considered if it is submitted at or after the lapse of the subject deadline.
       Option 2. Agreement to Share in Cost to Develop Data - Registrants may only choose
 this option for acute toxicity data and certainefficacy data and only if EPA has indicated in the
 attached data tables that your product and at least one other product are similar for purposes of
 depending on the same data" If this is the "case"" data may be generated for just one of the
 products in the group.  The registration number of the product for which data will be submitted
 must be noted in the agreement to cost share by the registrant selecting this option.  If you
 submitting the data. You must also provide EPA with documentary evidence that an agreement
 has been formed.  Such evidence may be your letter offering to join in an agreement and the
 other registrant's acceptance of your offer, or a written statement by the parties that an
 agreement exists.  The agreement to produce the data need not specify all of the terms of the
•"final aj^^ggj^gnf. Jietween'ffie parHes'of the mechanism to resolve the terms.  Section
 3(c)(2)(B) provides that if the parties cannot resolve the terms of the agreement they may
 resolve their differences through binding arbitration.

       Option 3. Offer to Share in the Cost of Data Development - This option only applies to
 acute toxicity and certain efficacy data as described in option 2 above.  If you have made an
 offer to  pay in an attempt to enter into an agreement or ^^nd ^ existing agreement to meet
 the requirements of this Notice and have been unsuccessful, you may request EPA (by selecting
 this option) to exercise its discretion not to suspend your registration(s), although you do not
 comply  with the data submission requirements of this Notice.  EPA has determined that as a
 general  policy, absent other relevant considerations, it will not suspend the registration of a
 product of a "registrant whb lias" in good faith sought and continues to seek to enter into a joint
 data development/cost sharing program, but the other registrant(s) developing the data has
 refused  to accept your offer. To qualify for this option, you must submit documentation to the
 Agency proving that you have  made an offer to another registrant (who has an obligation to
 submit data) to share ."In tfie g^en bl'developihg'tfiat data.  You must also submit to the
 Agency a completed EPA Form 8570-32, Certification of Offer to Cost Share in the
 Development of Data, Attachment 7.  In addition, you must demonstrate that the other
 registrant to whom the offer was made has not accepted your offer to enter into  a cost sharing
 agreement by including a copy of your offer and proof of the other  registrant's receipt of that
 offer (such as a certified mail receipt).  Your offer must, in addition to anything else, offer to
 share in the burden of producing the data upon terms to be agreed or failing agreement to be
 bound by binding arbitration as provided by FIFRA section 3(c)(2)(B)(iii) and must not qualify
 this offer.  The other fejgis'traiit "must also inform EPA of its election of an option to develop
 arid submit the data required by this Notice by submitting a Data Call-in Response Form and a
                                            184

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Requirements Status and Registrant's Response Form committing to develop and submit the
data required by this Notice.                                 '

       In order for you to avoid suspension under this option, you may not withdraw your
offer to share in the burdens of developing the data.  In addition, the other registrant must
fulfill its commitment to develop and submit the data as required by this Notice. If the other
registrant fails to develop the data or for some other reason is subject to suspension, your
registration as well as that of the other registrant will normally be subject to initiation of
suspension proceedings, unless you commit to submit, and do submit the required data in the
specified time frame.  In such cases, the Agency generally will not grant a time extension for
submitting the data.

       Option 4, Submitting an Existing Study - If you choose to submit an existing study in
response to this Notice, you must determine that the study satisfies the requirements imposed
by this Notice.  You may only  submit a study that has not been previously submitted to the
Agency or previously cited by anyone.  Existing studies are studies which predate issuance of,
this Notice. Do not use this option if you are submitting data to upgrade a study. (See Option

     /                         '                              . '
       You should be aware that if the Agency determines that the study is not acceptable, the
Agency will require you to comply with this Notice, normally without  an extension of the
required date of submission. The Agency may determine at any time that a study is not valid
and needs to be repeated.                                                     .

       To meet the requirements of the DCI Notice for submitting an existing study,  all of the
following three criteria must be clearly met:                   , .     '

       a.     You must certify at the time that the existing study is submitted that the raw data
             and specimens from the study are available for audit and review and you must
             identify where they are available.  This must be done in accordance with the
             requirements of the Good Laboratory Practice (GLP) regulation, 40 CFR Part
             160. As stated in 40 CFR 16Q.3(j) "  'raw data1 means any laboratory
            - worksheets, records, memoranda, notes, or exact copies thereof, that are the
             result of original observations and activities of a study and are necessary for the
             reconstruction and evaluation of the report of that study. In the event that exact
             transcripts of raw data have been prepared (e.g., tapes which have been
             transcribed verbatim, dated, and verified accurate by signature), the exact copy
     ;        or exact transcript may be substituted for the original source as raw data.  'Raw
             data' may include photographs, microfilm or microfiche copies, computer
             printouts, magnetic media, including dictated observations, and recorded data
             from automated instruments." The term "specimens", according to 40 CFR
             160.3(k), means  "any material derived from a test system for examination or
             analysis."                                                            v

      b.     Health and safety studies completed after May 1984 must also contain all GLP-
             required  quality assurance and quality control information, pursuant to the
             requirements of 40 CFR Part 160.  Registrants must also certify at the time of

                                         185

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              submitting Sie existing study that such GLP information is available for post-
              May 1984 studies by including an appropriate statement on or attached to the
              study signed by an authorized official or representative of the registrant.

              You must certify that each study fulfills the acceptance criteria for the Guideline
              relevant to the study provided in the FIFRA Accelerated Reregistration Phase 3
              Technical Guidance and that the study has been conducted according to the
              FesEcide Assessment Guidelines (PAG)  or meets the purpose of the PAG (both
              available from NTIS). A study not conducted according to the PAG may be
              submitted to the Agency for consideration if the registrant believes that the study
              clearly meets the purpose of the PAG. The registrant is referred to 40 CFR
              158.70 which states the Agency's policy regarding acceptable protocols. If you
              wish to submit the study, you must, in addition to certifying that the purposes of
                te PAG are met by the study, clearly articulate the rationale why you believe
                     I  I  I'Sli1!!1!,,"1!!!1!""!!'!!! ;i!"" "n...	in	:'.	•	i,	,"	*i a 	ปL	••_vป	" ""is1 r "' S""	'i	 J'S		A  	   •	
                e study meets the purpose of the PAG, including copies of any supporting
              mformation or data. It has been the Agency's experience that studies completed
              prior to January1970 rarely satisfied the purpose of the PAG and that necessary
              raw data are usually not available for such  studies.
              Till 11     I    ill, Jil  ,  i  , n        i      f 	    ,,i      i ,  ,  .   .
       If you submit an existing study, you must certify that the study meets all requirements
 Of the criteria outlined above.

       If you know of a study pertaining to any requirement in this Notice which does not
 meet the criteria outlined above but does contain factual information regarding unreasonable
 adverse effects, you must notify the Agency of such a study. If such study is in the Agency's
 files, you need only cite it along with the notification. If not in the Agency's files, you must
	submit a s^^^y ^^ c5pies	as requires	by' PR i^o'So" e 8 6-5.
       Option 5, Upgrading a Study — If a study has been classified as partially acceptable and
 upgradeable, you may submit data to upgrade that study.  The Agency will review the data
 submitted and determine if the requkement is satisfied. If the Agency decides the requirement
 is not satisfied, you may still be required to submit new data normally without any time
 extension. Deficient, but upgradeable studies will normally be classified as supplemental.
 However, it is Important: ^5 npte fEafriot all s^ies classified as supplemental are upgradeable.
 If you have questions regarding the classification of a study or whether a study may be
 upgraded, call or write the contact person listed in Attachment  1. If you submit data to
 upgrade an existing study you must satisfy or supply information to correct all deficiencies in
 the study identified by EPA.  You must provide a clearly articulated rationale of how the
 deficiencies have been remedied or corrected and why the study should be rated as acceptable
 to EPA. Your submission must also specify the MRID number(s) of the study which you are
 attempting to Upgrade and must be in confbrmance with PR Notice 86-5.

       Do not submit additional data for the purpose of upgrading a study classified as
 unacceptable and determined by the Agency as not capable of being upgraded.
                                           186

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        This option should also be used to cite data that has been previously submitted to
 upgrade a study, but has not yet been reviewed by the Agency. You must provide the MRID
 number of the data submission as well as the MRID number of the study being upgraded.

        The criteria for submitting an existing study, as specified in Option 4 above, apply to
 all data submissions intended to upgrade studies. Additionally your submission of data
 intended to upgrade studies must be accompanied by a certification that you comply with each
 of those criteria as well as a certification regarding protocol compliance with Agency
 requirements.

        Option 6,-Citing Existing Studies - If you choose to cite a study that has been
 previously submitted to EPA,xthat study must have been previously classified by EPA as
 acceptable or it must be a study which has not yet been reviewed by the Agency.  Acceptable
 toxicology studies generally will have been classified as "core-guideline" or "core minimum."
 For all other disciplines the classification would be "acceptable."  With respect to any studies
 for which you wish to select this option you must provide the MRID number of the study you
 are citing and, if the study has been reviewed by the Agency, you must provide the Agency's
 classification of the study.

       If you are citing a study of which you are not the original data submitter, you must
 submit a completed copy of EPA Form 8570-31, Certification with Respect to Data
 Compensation Requirements.

       Registrants who select one of the above 6 options must meet all of the requirements
 described in the instructions  for completing the Data Call-in Response Form and the
 Requirements  Status and Registrant's  Response Form, as appropriate.

 m-D REQUESTS FOR DATA WATVRRS

              If you request a waiver for product specific data because you believe it is
 inappropriate,  you must attach a complete-justification for the request, including technical
 reasons, data and references to relevant EPA regulations,  guidelines or policies.  (Note: any
 supplemental data must be submitted in the format required by PR Notice 86-5).  This will be
 the only, opportunity to state the reasons or provide information in support of your request.  If
 the Agency approves your waiver request, you will not be required to supply the data pursuant
 to section 3(c)(2)(B) of FIFRA. If the Agency denies your waiver request, you must choose an
 option for meeting the data requirements of this Notice within 30  days of the receipt of the
 Agency's decision.  You must indicate and submit the option chosen on the Requirements
 Status and Registrant's Response  Form. Product specific data requirements for product
 chemistry,  acute toxicity and efficacy  (where appropriate) are required for all products and the
 Agency would grant a waiver only under extraordinary circumstances. You should also be
 aware that submitting a waiver request will not automatically extend the due date for the study
 in question. Waiver requests submitted without adequate supporting rationale will be denied.
 and the original due date will remain in force.   •
                                                                        f
IV. CONSEQUENCES OF FAILURE TO COMPLY WITH THIS NOTICE
                                          187

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IV-A NOTICE OF INTENT TO SUSPEND

       The Agency may issue a Notice of Intent to Suspend products subject to this Notice due
io failure by a registrant to comply with the requirements of this Data Call-in Notice, pursuant
to FEFRA section 3(c)(2)(B).  Events which may be the basis for issuance of a Notice of Intent
to Suspend include, but are not limited to, the following:
              Failure to respond as required by this Notice within 90 days of your receipt of
              this Notice.
            I.


            2.     Failure to submit on the required schedule an acceptable proposed or final
                   protocol when such is required to be submitted to the Agency for review.

            3.     Failure to submit on the required schedule an adequate progress report on a
                   study as required by this Notice.

            4.     Failure ttoi submiton the required schedule acceptable data as required by this
                   Notice.

            5.     Failure to take a required action or submit adequate information pertaining to
                   any option chosen to address the data requirements (e.g., any required action or
                   information pertaining to 'suSmissibn"of•ciStlbnoT existing studies or offers,
                   arrangements^ or arbitration on the sharing of costs or the formation of Task
                   Forces, failure to comply with the terms of an agreement or arbitration
                   concerning joint data development or failure to comply with any terms of a data
                   waiver)'.    	'	,	•	  ,

            6.     Failure to submit supportable certifications as to the conditions of submitted
                   studies, as required by Section HL-C of this Notice.
     ii i        i  "tii i11'!,; JEM.'" (jSJiVi;1/ i!,;:;;!1	jt'jii •; lie l,tm i> wiim- in si i WM: it- it i;;"ซ. ; ',• i' •'; v - •, < s '".;"•
            7.     Withdrawal of an offer to share in the cost of developing required data.

            8.     Failure of the registrant to whom you have tendered an offer to share in  the cost
	      	      	Ojf jdeveioging data and provided proof of the registrant1 s receipt' of such  offer or
                   failure of a regis&ant on wliom you rely for a generic  data exemption either to:

                   a.     inform EPA of intent to develop and submit the data required by  this
                          Notice on a Data Call-in Response Form and a Requirements Status and
                          Registrant's Response Form:

                   b.     fulfill the commitment to develop and submit the data as required by this
                          Notice; or	

                   c.     otherwise take appropriate steps to meet the requirements stated in this
                          Notice, unless you commit to submit and do submit the required  data in
                          the specified time frame.
                                           188

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        9.     Failure to take any required or appropriate steps, not mentioned above  at any   •
               time following the issuance of this Notice.

 IV-B.  BASIS FOR DETERMINATION THAT STTR1UTTTT?r> STUDY TS
 UNACCEPTABLE'              ~—"	~

        The Agency may determine that a study (even if submitted within the required time) is
 unacceptable and constitutes a basis for issuance of a Notice of Intent to  Suspend  The grounds
 for suspension include, but are not limited to, failure to meet any of the following:

        1.  EPA requirements specified in the Data Call-in Notice or other documents
        mcorporated by reference (including, as applicable, EPA Pesticide Assessment
        Guidelines, Data Reporting Guidelines, and GeneTox Health Effects Test Guidelines.)
        regarding the design, conduct, and reporting of required studies.  Such requirements
        include, but are not limited to, those relating to test material, test procedures, selection
        of species, number of animals, sex and distribution of animals,  dose and effect levels to
        be tested or attained, duration of test, and, as applicable, Good Laboratory Practices.

        2.  EPA requirements regarding  the submission  of protocols, including the
        incorporation of any changes required by the Agency following review.   *

        3.  EPA requirements regarding-the reporting of data, including the manner of
        reporting, the completeness of results, and the adequacy of any required supporting (or
        raw) data, including, but not limited to, requirements referenced or included in this
        Notice or contained in PR 86-5.  All studies must be submitted in the form of a final
        report; a preliminary report will not be considered to  fulfill the  submission requirement.

 IV-C EXISTING STOCKS OF SUSPENDED OR CANCP.LLED PRODUCTS

        EPA has statutory authority to permit continued  sale, distribution and use of existing
 stocks of a pesticide product which has been suspended  or cancelled if doing so would be^
 consistent with the purposes of the Act.

       The Agency has  determined that such disposition by registrants of existing stocks for a
 suspended registration when a section 3(c)(2)(B) data request is putstanding would generally
 not be consistent with the Act's purposes.  Accordingly, the Agency anticipates granting
 registrants permission to sell, distribute,  or use existing  stocks of suspended product(s) only in
 exceptional circumstances.  If you believe such disposition of existing stocks of your productfs)
 which may be suspended for failure to comply with this  Notice should be permitted, you have
 the burden of clearly demonstrating to EPA .that granting such permission would be consistent
 with the Act.  You must also explain why an "existing stocks" provision is necessary, including
 a statement of the quantity of existing stocks and your estimate of the time required for their
 sale, distribution, and use.  Unless you meet this burden the Agency will not consider any
request pertaining to the continued sale, distribution, or  use of your existing stocks after
suspension.
                                          189

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       If you request a voluntary c^celiafibn'ofybuf product(s)"as a response to this Notice
and your product is in full compliance with all Agency requirements, you will have, under
most circumstances, one year from the date your 90 day response to this Notice is due, to sell,
distribute, or use existing stocks. Normally, the Agency will allow persons other than the
registrant such as independent distributors, retailers and end users to sell, distribute or use such
existing stocks until the stocks are exhausted. Any sale,  distribution or use of stocks of
voluntarily cancelled products containing an  active ingredient for which the Agency has
particular risk concerns will be determined on case-by-case basis.

       Requests for voluntary cancellation received after the 90 day response period required
by this Notice will not result in the Agency granting any additional time to sell, distribute, or
use existing  stocks beyond a year from the date  the 90 day response was due unless you
deirjohstrate to the Agency that you are in full compliance with all Agency requirements,
including the requirements of this Notice. For exafnple,  if you decide to voluntarily cancel
your registration six months before a 3 year  study is scheduled to be submitted, all progress
reports and other information"necessary to establish that you have been conducting the study in
an acceptable and good faith manner must have  been submitted to the Agency, before EPA will
consider granting an existing stocks provision.

SECTION V.  REGISTRANTS' OBLIGATION TO REPORT POSSIBLE UNREASONABLE
ADVERSE EFFECTS

       Registrants are reminded that FIFRA section 6(a)(2)  states that if at any time after a
pesticide is registered a registrant has additional factual information regarding unreasonable
adverse effects on the environment by the pesticide,  the registrant shall submit the information
to the Agency.  Registrants must notify the Agency of any factual information they have, from
whatever source, including but not limited to interim or preliminary results of studies,
regarding unreasonable adverse effects on man or the environment.  This requirement
continues as long as the products are registered  by the Agency.
 SECTION VI.  INQUIRIES AND RESPONSES TO THIS NOTICE

       If you have any questions regarding the requirements and procedures established by this
 Notice, call the contact person(s) listed in Attachment 1, the Data Call-in Chemical Status
                                          190

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       All responses to this Notice (other than voluntary cancellation requests and generic data
 exemption claims) must include a completed Data Call-Tn Response Form and a completed
 Requirements Status and Registrant's Response Form (Attachment 2 and Attachment 3 for
 product specific data) and any other documents required by this Notice, and should be
 submitted to the contact ,person(s) identified in Attachment 1.  If the voluntary cancellation or
 generic data exemption option is chosen, only the Data Call-in Response Form need be
 submitted.

       The Office of Compliance Monitoring (OCM) of the Office of Pesticides and Toxic
 Substances (OPTS), EPA, will be monitoring the data being generated in response to this
 Notice.  ,
                                        Sincerely yours,

                                        Lois A. Rossi, Director
                                        'Special Review and
                                         Reregistration Division
Attachments

       1  -
       2  -
       3  -
       4  -

       5  -
       6  -
Data Call-in Chemical Status Sheet
Product-Specific Data Call-in Response Form
Requirements Status and Registrant's Response Form
EPA Batching of End-Use Products for Meeting Acute Toxicology Pat;
Requirements for Reregistration
List of Registrants Receiving This Notice
Cost Share and Data Compensation Forms and the Confidential -Statp.me
Formula Form
                                         191

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I'!FI;I	(IP1:; :,!  3     !K ,   IIIH   i,"
                                                                        •./>.  ', i;•  ;<	is.•;!;  ifi'fE;!1 M!:•",  .1	11-i:.• -Ii'i'/ii'i'S"!:;-:".'c-i1,;;!Kliin''.'  ป,;:if'i	i-'iiii',.;;vi?.s,in-	• IB	cj;'
                                                                                                                              H |il,|  '",,!''!" H! ',,'iii!.,,,! •  ''it1   , i  lii,!	IE'i1,.:,!!1	i1,,,.''!, ,, J  .l
                                                                                                                                          192

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 THIOBENCARB DATA CALL-IN CHEMICAL STATUS SHEET

 INTRODUCTION       ,                 '

       You have been sent this Product Specific Data Call-in Notice because you have productfs)
 containing thiobencarb.                                                    .

     .  This Product Specific Data Call-in Chemical Status Sheet: contains an overview of data
 required by this notice,, and point of contact for inquiries pertaining to the reregistration of
 thiobencarb. This attachment is to be used in conjunction with (1) the Product Specific Data Call-
 in  Notice, (2) the Product Specific Data Call-in Response Form (Attachment 2)  (3) the
 Requirements Status and Registrant's Form (Attachment 3), (4) EPA's Grouping of End-Use
 Products  for  Meeting Acute Toxicology  Data Requirement (Attachment  4), (5) the EPA
 Acceptance Criteria (Attachment 5), (6) a Hst of registrants receiving this DCI (Attachment 6) and
 (7) the Cost Share and Data Compensation Forms in replying to this 2665 Product Specific Data
 Call-In (Attachment 7). Instructions and guidance accompany each form.

 DATA REQUIRED BY THIS NOTTCR

       The additional data requirements needed to complete the database for 2665 are contained
 *? ฑe Requirements Status  and Registrant's Response Attachment 3. The Agency has concluded
 that additional data on 2665 are needed for specific products. These data are required to be
 submitted to the Agency within the time frame listed. These data are needed to fully complete
 the reregistration of all eligible 2665 products.                               •

INQUIRIES AND RESPONSES TO THIS NOTTCR

       If you have any questions regarding this product specific data requirements and procedures
established by this Notice, please contact CP Moran at (703) 308-8590.

       All responses to this Notice for the Product Specific data requirements should be submitted
       to:                 .    .
             CP Moran
             Chemical Review Manager Team  81
             Product Reregistration Branch
             Special Review and Reregistration Branch 7508W
             Office of Pesticide Programs
             U.S. Environmental Protection Agency
             Washington, D.C. 20460

             RE: 2665
                                        193

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                                                                                                                                     194

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I, '!"	ii'lli'l1'1'..,!!,!!!1 !.i,,ih
            INSTRUCTIONS FOR COMPLETING THE DATA CALL-IN RESPONSE FORM FOR
                                        PRODUCT ^SPECmC DATA	

           Item 1-4.     Already completed by EPA.
                 ,t
           Item 5.       If you wish to voluntarily cancel your product, answer "yes."  If you choose this
                        option, you will not have to provide the data required by the Data Call-in Notice
                        and you will not have to complete any other forms.  Further sale and distribution
                        of your product after the effective date of cancellation must be in accordance with
                        the Existing Stocks provision of the Data Call-in Notice (Section IV-C).

           Item 6.       Not applicable since this form  calls in product specific data only.  However, if
                        your product is identical to another product and you qualify for a data exemption,
                        you must respond with "yes" to Item 7a (MUP) or 7B (EUP) on this form, provide
                        the EPA registration numbers of your source(s); you would not complete the
                        "Requirements  Status and Registrant's Response" form.   Examples of such
                        products include repackaged products and Special Local Needs (Section 24c)
                        products which are identical to  federally registered products.

           Item 7a,     For each manufacturing use product (MUP) for which you wish to maintain
                        registration, you must agree to satisfy the data requirements by responding "yes."
           ^l iiipi!' !  I-1"  .' ,„ i. •';: i;, i.;,,. '; ,,n, i •" •' ' iniliiiQiliii f i' '"iii1,,,.':, ป•, ,,ป' i;,,;, „ f	i, j 4i!' ;i;!' I,'1' Til1	iiJ ฐ.i • i HI, •'!''", „•" i',:!!1'"' i ii1	Mil .i!!!ll t1 i !> 1,,' ft is I ''"Si* ::! •[	 '"'.'"'i1''" i: I ffl!,!:' "V> '^ H* if ill'i:'ซ' • i, • •: ''fcii; if* • B i'',",' : ป' "il' •.  • •':"'" i'."" i;> , ?   ' . •   h ,  ' , ,' ' '
           ItenYTb.
 For each end use product (EUP) for which you wish to maintain registration, you
:.musf 'M^^*^"^	^e"	d"*^	requirehiientis' by responding "yes."  If you  are
.^questing1	a	data"waiver"	answer ""'yes1" nere; in addition, on the "Requirements
 Status and Registrant's Response" form under Item 9, you must respond with
 Option 7 (Waiver Request) for each study for which you are requesting a waiver.
 See Item 6 with regard to identical products and data exemptions.
            Items 8-11. Self-explanatory.
                                                                iilhililllllilh' .rfM.'lillliJilil'"!!!'''!!!1: ' 'I, hil. •'!', ' f1,1  ii'   i ' 'll .' ' r ' Iii  '      '^ 	!•' :'i I '
            NOTE:       You may provide additional information that does not fit on this form in a signed
                         letter that accompanies this form. For example, you may wish to report that your
                         product has already been transferred to another company or that you have already
                         voluntarily canceled this product.  For these cases, please supply all relevant
                         details so that EPA can ensure that its records are correct.
                                                      196

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 Item 1-3
 Item 4.
™^TC.™   :FOR COMpLETING THE REQUIREMENTS STATUS AND
REGISTRANT'S RESPONSE FORM FOR PRODUCT SPECIFIC DATA

     Completed by EPA. Note the unique identifier number assigned by EPA in Item
     3   This number must be used in the transmittal document for any  data
     submissions in response to this Data Gall-In Notice.

     The  guideline reference numbers of studies required to support  the product's
     continued  registration are identified.   These guidelines, in  addition to the

     STSStSS?IJ1^^0^^ CฐMuCt <*?ป ^nired ^ies.
 Item 5.

 Item 6.
Item 7.
Item 8.
Item 9.
                                               t

     The study title associated, with the guideline reference number is identified.

     The use patternฎ of the pesticide associated with the product specific requirements
     is (are) identified.  For most product specific data requirements, all use patterns
     are covered by the data requirements.  In the case of efficacy data, the required
     studies only pertain to products which have the use sites and/or pests indicated.

    -The substance to be tested is identified by EPA.  For product specific data, the
    product as formulated for sale and distribution is the test substance, except in rare
    The due date for submission of each study is identified. It is normally based on
    8 months after issuance of the Reregistration Eligibility Document unless EPA
    determines that a longer time period is necessary.

    Enter only one of the following response codes for each data rPT,^™0^ to
    show how you intend to comply with the data requirements listed in this table
    duller descriptions of each option are contained in the Data Call-in Notice.

    I will generate and submit data by the specified due date (Developing Data)  Bv
    indicating that I have chosen this option, I certify that I will comply with all the
    requirements pertaining to the conditions for submittal of this study as outlined in
    the  Data^ Call-in  Notice.  By the specified  due date, I will also  submit:  (1) a
    completed "Certification With Respect To Data Compensation Requirements"
    form (EPA Form 8570-29) and (2) two completed and  signed copies of  the
    Confidential Statement of Formula (EPA Form 8570-4) .

    I have entered into an agreement with one or more registrants to develop data
   Jr?v( CostSharmง)-  l am submitting a copy of this agreement,  I understand
    lhat this option is available only for acute toxicity or certain efficacy data and only
   if.EPAuidicates in an attachment to this Notice that my product is similar enough
   to another  product to qualify for this option.  I certify that another party in the
   agreement is committing to submit or provide the required data; if the required
   study is not submitted  on time, my product may be subject to suspension  By the
   specified due date, I will also  submit: (1)  a completed "Certification With
                                        201

-------
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                           nil          i in     i               ii  i i ii i i  i , in ill n i II  I __ '/'•.i-i'.™" ..... ia '•
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                          (2) two completed and signed copies of the Confidential Statement of Formula
                          '(EPA ..... Form 8570-4) . \ ..... '~"_ '"' """ ' ..... ' ...... " ^ ..... ; "' ...... [ ..... " "~ "'__ ..... " ^ "' ™' ™ .....
                                                                     ^
                                                                    1 *.**ซ' ..... .S;'1-:,
      I have made offers to share in the cost to develop data (Offers to Cost Share).
      I understand that this option is available only for acute toxicity or certain efficacy
      data and only if EPA indicates in an attachment to this Data Call-In Notice that my
      product is similar enough to another product to qualify for this option.  I am
      submitting evidence that I have made an offer to another registrant (who has an
      obligation to submit data) to share in the cost of that data. I am also submitting a
      completed "Certification of Offer to  Cost Share in the  Development Data"
      form.  I am inducting a copy of my offer and proof of the other registrant' s receipt
      o'f mat offer. "1 "am i^u^^'i^pa^'whidi is committing to submit or provide
      the required data; if the required  study is not submitted on time, my product may
      be subject to suspension.  I understand that other terms under Option 3 in the Data
      Call-In Notice (Section ni-C.l.) apply as well. By the specified due date,  I will
      also submit: (1) a completed "Certification With Respect To Data Compensation
      Requirements" form (EPA Form 8570-29) and  (2) two completed and signed
      copies of the Confidential Statement of Formula (EPA Form 8570-4).
                    ^ '.l-'.'gy'^'^^^j^^	submit	an"" existing'study that has not been
                           submitted previously to the Agency by anyone (Submitting an Existing Study).
                           f cerHfy Sat this suidy will meet all the requirements for submittal of existing data
                           Outlined in Option 4 in the Data Call-In Notice (Section HI-C. 1.) and will meet the
                           Ittached acceptance criteria (for acute toxicity and product chemistry data). I will
                           attach the needed supporting information along with this response.  I also certify
      "that I 'Haveae"terminedmat this study will fill the data requirement for which I have
       indicated this choice.  By the specified due date, I will also submit a completed
       "CeS^SK^^I^St^oData CompensationRequirements" form (EPA
       Form 8570-29) to show what data compensation option I have chosen.  By the
       specified due date,  I wM also submit:'. (1) a completed "Certification With
       Respect To Data Compensation Requirements" form (EPA Form 8570-29) and
       (2) two c0mpieted and signed copies of the Confidential Statement of Formula
       (EPA Form 8570-4).

 5.     By  the specified due date, I will submit or cite data to upgrade a study classified
       by the Agency as partially acceptable and upgradable (Upgrading a Study). I will
       Submit evidence of the Agency's review indicating that the study may be upgraded
       and what information is required to do so.  I will provide the MRID or Accession
       number of the study at the due date.  I understand that the conditions for this
       option outlined Option 5 in the Data  Call-In Notice (Section DI-C.l.) apply.  By
       the specified due date, I will also submit: (1) a completed "Certification With
       Respect To Data Compensation Requirements" form (EPA Form 8570-29) and
       (2) two completed and signed copies  of the Confidential Statement of Formula
       (EPA Form 8570-4).

 6.    By the specified due date,  I will cite an existing  study that the  Agency  has
^V""ijf Classified as acceptable or an existing study that has been submitted  but not
                                                       ,202

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       7.
  r,Z  ?•  I*^e Agency (Citmg an Existing Study).  If I am citing another
  registrant s study, I understand that this option is available only for acute toxicity
  or certain efficacy data and only if the cited study was conducted on my product
  an identical product or a product which EPA has "grouped" with one or more
  other products for purposes of depending on the same data.  I may also choose this
  option^if I am citing my own data.  In either case, I will provide the MRTO or
  Accession numberฎ for the cited data on a "Product Specific Data Report" form
  or in a similar format.'  By the specified due date, I will also submit" (1) a
  completed "Cerfafication With Respect To Data Compensation Requirements"
  form (EPA Form  8570-29)  and (2) two completed and signed copies of the
  Confidential Statement of Formula (EPA Form 8570-4).

 I request a waiver  for this  study because it is inappropriate for  my product
 (Waiver Request).   I am attaching a Complete justification for this request
 including technical  reasons,  data and references to  relevant EPA  regulations'
 guidelines or policies.  [Note: any supplemental data must be submitted in the
 format required  by P.R. Notice 86-5].   I understand  that this is my only
 opportunity to state the reasons or provide information in support of my request
 If the Agency approves my waiver request, I will not be required to supply the
 data pursuant to Section 3(c)(2)(B) of FIFRA. If the Agency denies my waiver
 request I must choose a method of meeting the data requirements of this Notice
 by the due date stated by this Notice. In this case, I must, within 30 days of my
 receipt of the Agency's written decision, submit a revised "Requirements Status
 and Registrant-s Response" Form indicating the option chosen.  I also understand
 that the deadline for submission of data as specified by the original data call-in
 notice will  not change.  By  the specified due date,  I will also  submit: (1) a
 completed "Cerfafication With Respect To Data Compensation Requirements"
            ^T 857ฐ>9)  ^ (2) ^ comPle
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TRB'S BATCHING OF PRODUCTS CONTAINING THIOBENCARB AS THE ACTIVE
INGREDIENT  FOR  MEETING  ACUTE TOXICITY DATA REQUIREMENTS  FOR
REREGISTRATION
                                                                                      i
       In an effort to reduce the time, resources and number of animals needed to fulfill the acute
toxicity  data  requirements  for  reregistration of products containing  the active  ingredient
Thibencarb  (S-((4~Chlorophenyl)methyl) N,N-diethylthiocarbamate) the Agency has batched
products which can be considered similar in terms of acute toxicity.  Factors considered in the
sorting process include each product's active and inert ingredients (identity, percent composition
lid biological activity), product form (liquid, paste, solid, etc.), and labeling (e.g., signal word,
precautionary labeling, etc.).

       Using available iiiformation7 batching nag been accompHshed by the process described in
the preceding paragraph. Notwithstanding the batching process, the Agency reserves the right to
require, at any time, acute toxicity data for an individual product should the need arise.

       Registrants of products within a batch may choose to cooperatively generate, submit or cite
a single battery  of six acute toxicological studies to represent all the products within that batch.
Registrants have the option of participating with all or some other registrants of products in theix
product's batch, to deal only their own products within a batch, or to generate all the required
acute toxicological studies for each of their own products. If a registrant chooses to generate the
data for a batch, he or she must use one of the products within the batch as the test material. If
 a registrant chooses to rely upon previously submitted acute toxicity data, he or she may do so
 provided that the data base is complete and valid by today's standards (see the attached acceptance
 criteria), the formulation tested is considered by EPA to be similar for acute toxicity, and Hie
 formulation has  not been significantly altered since submission and acceptance  of the acute toxicity
                ; mav not support their product using data conducted on a product from a. different
 data, R
 batch!	TR1B
III I H ,,'H H, " i; U'INL	'i"	'ป	i,,i
                   not support their product	
                       ' anv new of caffffeled formnlations (that were presented to the Agency
          UL,ii.^ป.. of the RED1 before data derived from them can be nsp.H tn cover other
,.,wป^i.. a batch.  Regardless of whether new data is generated or existing data is referenced,
registrants mu^Tde"arlv identify the test material bv EPA Registration Number. If more than one
confidential statement of formula (CSF) exists for a product, the registrant must indicate the
formulation actually tested by identifying the corresponding CSF.
I      I       11111  I   I    II Illl III II I   I | I I  I II III I III  I  III   Illl 1 I II III II  I  III I I  I     ,"' \ i •' , ,  '    y  ' '   ป'>! < iil"'i'1 !. ,
       In deciding how to meet the product specific data requirements, registrants must follow
the directions given in the Data Call-In Notice and its attachments appended to the RED.  The DCI
Notice contains two response forms which are to be completed and submitted to the Agency within
90 days of receipt.  The first form, "Data Call-In Response," asks whether the registrant will meet
the data requirements for each product.  The second form, "Requirements Status and Registrant s
Response," lists the product specific data required for each product, including the standard six
acute toxicity tests.  A registrant who wishes to participate in a batch must decide whether he or
She will provide the data or depend on someone else to do so. If a  registrant supplies the data to
support a batch of products, he or she must select one of the following options: Developing Data
(Option 1), Submitting an Existing Study (Option 4), Upgrading an Existing Study (Option 5) or
Citing an Existing Study (Option 6).  If a registrant depends on another's data, he or she must
choose among: Cost Sharing (Option 2), Offers to Cost Share (Option 3) or Citing an Existing
Study (Option 6). ff a"reglsttaiit"does not want to participate in a batch, the choices are Options
 1 4, 5 or 6.  However, a registrant should know that choosing not to participate in a batch does
                                            204

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(Option 3) those studies.



Table 1 displays the products that were batched.


Table 1.
                          * "" batth fr0m-cWn8 ™ or her ซ"*• aud offering to cost share
Batch
1

2

Registration Number
59639-79
— 	 	 	 	 . 	 _
63588-6
59639-80
	 • — 	 	
63588-5
Percent Active Ingredient
Thiobencarb • 84%
Thibencarb 34%
Thibencarb ...10%
— 	 	 	 	 . 	 	
Thiobencarb 10%
	 — 	 _ — .
Form
liquid
liquid
solid
	 	 	 ' 	 , —
solid
Table 2 displays the product that was not batched.  The registrant is responsible for submittine

SiS3*     t0, ^r ?S Pr0dUCt' The Te^nical Review Branch wiU not accepfrSewf
submitted on any of the batched products to support the one "No Batch" product.
Table 2.
                  63588-4
                                                      Percent Active Ingredient
                                            Thiobencarb
                                                                             ...97.4%
                                       205

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206

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              i ihKilli1!!!!' ' lit*'"1!,,
Instructions for Completing the Confidential Statement of Formula

The Confidential Statement of Formula (CSF) Form 8570-4 must be used. Two legible, signed
fippies of the form are required. Following are basic instructions:

       a.     All the blocks on the form must be filled in and answered completely.

       b.     If any block is not applicable, mark it N/A.

       c<     The CSF must be signed, dated and the telephone number of the responsible party
:	::	: '• • •' '  ••  • • must be provided!

       d.     All applicable information which is on the product specific data submission must
             also be reported on the CSF.

       e.     .JsJii'wsigfas^                 7 must be in pounds per gallon for liquids and
             pounds per cubic feet for solids.

       f.     Flashpoint must be in degrees Fahrenheit and flame extension in inches.

       g.    For all active ingredients,  the EPA Registration Numbers for  the  currently
             registered source products must be reported under column 12.
                                                                              !      ....
       h.    The Chemical Abstracts Service (CAS) Numbers for all actives and inerts and all
             common names for the trade names must be reported.

       i.     For the active ingredients,  the percent purity of the source products must be
             reported under column  10 and must be exactly the same as on the source product's
             label.
       j.     All the weights in columns 13.a. and 13.b. must be in pounds, kilograms, or
              grams. In no case will volumes be accepted. Do not mix English and metric system
              units (i.e., pounds and kilograms).

       k.     All the items under column 13.b: must total 100 percent.

        1.     All items under columns 14.a. and 14.b. for the active ingredients must represent
™" "   .'"	,,';	pure'active form.

        m.    The upper and lower certified limits for aU active and inert ingredients must  follow
              the <40 CFR 158.175 instructions. An explanation must be provided if the proposed
              limits are different than standard certified limits.

        n.     When  new CSFs are  submitted and approved,  all previously submitted CSFs
              become obsolete for that specific formulation.
                                       ,   208

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                                         209

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•I" li  i  ,,i!"M!|!|'i  'i r ' 'ill'
                                                                                                   :.i II'"1!1 t "Mi. ,11'   '   Illi" '!i,i,  "K ''i!!1 h  ">i 9\'Xซ"\ 't  I!  'i1',, .liil1 ,S HI
                                                                                                                                                    210

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                    United States Environmental Protection Agency
                               Washington, D.C. 20460
                           Certification  of Offer to Cost
                        Share in the Development of Data
  Form Approved
OMB No. 2070-0106,
    2070-0057
 Approval Expires
     3-31-99
 anagement and Budget, Paperwork Reduction                                       '

 ease fill in blanks below:
Impany Namo
bduct Name
 lertify that:


Company Number
EPA Reg. No.
S

lature of Company's Authorized Representative
le and Title (Please Type or Print)
=orm 8570-32 (5/91) Replaces EPA form 8580 which is obselete
                                                                         Date
                                          211

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212

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                       United States Environmental Protection Agency
                                   .Washington, DC 20460
                        CERTIFICATION WITH RESPECT TO
                     DATA COMPENSATION REQUIREMENTS
Form Approved
OMB No. 2O70-O107,
2070-0057
Approval Expires
3-31-99
 lublic reporting burden for this collection of information is estimated to average 15 minutes per response including timp for
                                   ^^^
        suggestions for reducing this burden to, Chief Information Policy Branch  PM-233 u S                         '
 [lease fill in blanks below.
lompany Name
 roduct Name
 Certify that:
                                                                                              Company Number
                                                                                              EPA Reg. No.
                                                                                                                 '•
    That for each study cited in support of registration or reregistration under  FIFRAthat is NOT an exclusivp UQ* <**,,*, i *™  th


                                                                                                     the attached
 gnature
 une and Title (Please Type or Print)
                                                                                             Date
                                                to pay compensation to other persons, with regard to the registration or
                                                FIFRA section 3(c)(1)(F) and 3(c)(2)(D).                       ,     ..:
gnature
arne and Title (Please Type or Print)
Form 8570-31 (4-96)
                                                     213

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               Ulii
214

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    The following is a list of available documents for thiobencarb that may further assist you in
responding to this Reregistration Eligibility Decision document.   These documents may be
obtained by the following methods: "•*/••

Electronic
File format:  Portable Document Format (.PDF) Requires Adobeฎ Acrobat  or  compatible
             reader.  Electronic copies can be downloaded from the Pesticide Special Review
             and Reregistration Information System at 703-308-7224.  They also are available
             on the Internet using WWW (World Wide Web) on WWW.EPA.GOV., or contact
             CP Moran at (703)-308-8590.
    1.
PR Notice 86-5.
    2.       PR Notice 91-2 (pertains to the Label Ingredient Statement).

    3.       A full copy of this RED document.   .

    4.       A copy of the fact sheet for 2665.


    The following documents are part of the Administrative, Record for 2665 and may included
in the EPA's Office of Pesticide Programs  Public Docket. Copies of these documents are not
available electronically, but may be obtained by contacting the person listed on the Chemical
Status Sheet.

    1.       Health and Environmental Effects Science Chapters.
                                     '            ^-       .

    2.       Detailed Label Usage Information System (LUIS) Report.

    The following Agency reference documents are not available electronically, but may be
obtained by contacting the person listed on the Chemical Status Sheet of this RED document.

    1.       The Label Review Manual.

    2.       EPA Acceptance Criteria
                                         215

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                                                                                                         .'iTJi^ililli  '$!&/
                                                                                                         ' MS!'	  "I- 'I'..'!  , .I1?,!;!,   .fJH! ';",,•>".
                                                                                              216

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