Comparison of VOA Compositing Procedures September 1995 U.S. Environmental Protection Agency Office of Water Office of Science and Technology Engineering and Analysis Division (4303) 401 M Street SW Washington, DC 20460 ------- Comparison of VOA Compositing Procedures Acknowledgments This study described in this report was performed under the direction of William A. Telliard of the Engineering and Analysis Division (EAD) within the U.S. Environmental Protection Agency's (EPA's) Office of Water. This report was prepared under EPA Contract 68-C3-0337 by DynCorp Environmental, with the assistance of Interface, Inc. The authors wish to thank Pacific Analytical, Inc. for the isotope dilution GC/MS analyses and Isco, Inc. and Associated Design and Manufacturing Co. for training and assistance in the use of their automated samplers. Disclaimer This report has been reviewed and approved for publication by the Engineering and Analysis Division of the U.S. Environmental Protection Agency. Mention of company names, trade names, or commercial products does not constitute endorsement or recommendation for use. Further Information For further information, contact: William A. Telliard USEPA Office of Water Engineering and Analysis Division (4303) 401 M Street SW Washington, DC 20460 Phone: 202-260-7120 Fax: 202-260-7185 ------- Comparison of VOA Compositing Procedures SUMMARY This report provides results produced by VOA grab and composite sampling procedures in studies conducted by the U.S. Environmental Protection Agency (EPA) in early 1994. In these studies, four individual grab samples of real-world effluents were collected over the course of a day. These samples were analyzed spiked or unspiked, composited and individually, by isotope dilution GC/MS, using Revision C of EPA Method 1624. Both manual and automated grab sampling procedures were employed. Compositing procedures employed included flask, purge device, and continuous. Analytes spiked were the volatile organic GC/MS fraction of the priority pollutants plus additional compounds routinely tested for in EPA's industrial surveys. The objective of these studies was to compare the analytical results for manually collected individual grab samples to the analytical results for composited samples and automatically collected grab samples, to determine if bias occurred in the automated grab and compositing processes. Several compositing methods were investigated including: flask compositing and purge device compositing of automated and manual grab samples, and continuous compositing. These tests showed that, for the samples tested, the mathematical average of the analytical results for hand collected grab samples were higher than results for composited samples. Conversely, mathematical averages of the analytical results for hand collected grab samples were marginally lower than results of automated grab samples. The cause of these slight differences is not known. However, the differences are not significant when compared to the variability in the analytical technique. It is not likely that these differences would have been found using a less sensitive analytical technique than isotope dilution GC/MS. September 1995 ------- ------- Comparison of VOA Compositing Procedures Background Volatile Organic Compounds (VOCs) The Federal Water Pollution Control Act of 1972 (PL 92-500) required EPA to control the discharge of toxic pollutants to the nation's waters. The act listed 65 compounds and compound classes for regulation as toxic pollutants. This list was later refined into an initial list of 129 "priority pollutants" and then a final priority pollutant list containing 126 individual compounds (Reference 1). ' For determination of the priority pollutants, EPA separated the list of 126 compounds into groups based on the analytical technology that could be used to measure the pollutants. Those organic pollutants that could be determined by gas chromatography combined with mass spectrometry (GC/MS) were further categorized into volatile and acid/base/neutral extractable fractions. The volatile fraction, also called the "purgeable" fraction, contains those compounds that boil below approximately 130 °C and that are capable of being purged from water using a flowing gas stream (Reference 2). Analysis of this fraction is termed a "volatile organic analysis" (VOA) and the compounds in this fraction are termed "volatile organic compounds" (VOCs). Determination of VOCs in the VOA fraction of the list of priority pollutants is the subject of this study. Pollutant Lists A list of VOCs analyzed in this study is provided in Table 1. This table also provides a list of the stable isotopically-labeled analogs that were used for isotope dilution quantitation, and information concerning whether a given analyte is a priority pollutant or other pollutant associated with the 1976 Consent Decree (Reference 1). Chemical Abstracts Service Registry Numbers for the pollutants and their labeled analogs are given, where available. The VOCs listed in Table 1 are separated into two groups. The first group contains VOCs that are determined by GC/MS using authentic standards; the second group contains VOCs determined by "reverse search." These latter compounds are considered identified when the chromatographic retention time and mass spectrum agree with those specified in the method. When a match is found, the compound is quantitated based on a response factor also given in the method. Although results produced by the reverse search technique are not as precise or accurate as results produced using authentic standards, the technique is useful for screening and provides approximate concentrations for VOCs in the reverse-search group. Furthermore, reverse search is more accurate in identifying compounds than a forward library search in which only the mass spectrum is tested against a large mass spectral file. September 1995 ------- Comparison of VOA Compositing Procedures Table 1 Volatile Organic Compounds Analyzed Compounds Determined by Isotope Dilution or Internal Standard Compound Acetone Acrolein Acrylonitrile Benzene Bromodichloromethane Bromoform Bromomethane Carbon tetrachloride Chlorobenzene Chloroethane 2-Chloroethylvinyl ether Chloroform Chloromethane Dibromochloromethane 1,1-Dichloroethane 1,2-Dichloroethane 1,1-Dichloroethene trans-1 ,2-Dichloroethene 1 ,2-Dichloropropane trans-1 ,3-Dichloropropene Diethyl ether p-Dioxane Ethylbenzene Methylene chloride Methyl ethyl ketone (MEK) 1 ,1 ,2,2-Tetrachloroethane Tetrachloroethene Toluene 1,1,1-Trichloroethane 1 ,1 ,2-Trichloroethane Trichloroethene Vinyl chloride CAS Registry 67-64-1 107-02-8 107-13-1 71-43-2 75-27-4 75-25-2 74-83-9 56-23-5 108-90-7 75-00-3 110-75-8 67-66-3 74-87-3 124-48-1 75-34-3 1 07-06-2 75-35-4 1 56-60-5 78-87-5 10061-02-6 60-29-7 123-91-1 100-41-4 75-09-2 78-93-3 79-34-5 127-18-4 109-88-3 71-55-6 79-00-5 79-01-6 75-01-4 Labeled Compound Analog d6 d4 d3 d6 13C 13/^ \j d3 ,3C d5 d5 13C d3 13C d3 d4 d2 d3 d6 d4 dio d8 dio d2 d3 d2 i3r> 02 d8 d3 13c2 13/^ O2 d3 CAS Registry 666-52-4 33984-05-3 53807-26-4 1076-43-3 93952-10-4 72802-81-4 1111-88-2 32488-50-9 3114-55-4 19199-91-8 31717-44-9 1111-89-3 93951-99-6 56912-77-7 17070-07-0 22280-73-5 42366-47-2 93952-08-0 93951-86-1 2679-89-2 17647-74-4 25837-05-2 1665-00-5 53389-26-7 33685-54-0 32488-49-6 2037-26-5 2747-58-2 93952-09-1 93952-00-2 6745-35-3 .Priority^ Pollutant N Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y N N Y Y N Y Y Y Y Y Y Y September 1995 ------- Comparison of VOA Compositing Procedi Compounds Determined by Reverse Search Compound Carbon disulfide cis-1 ,3-Dichloropropene 2-Hexanone 4-Methyl-2-pentanone Trichlorofluoromethane Vinyl acetate /n-Xylene o- and p-Xylene CAS Registry 75-15-0 10061-01-5 591-78-6 108-10-1 75-69-4 108-05-4 108-38-3 * Priority Pollutant N Y N N N N N N * O-xylene CAS Registry = 95-47-6 P-xylene CAS Registry = 106-42-3 In addition to the priority pollutant VOCs, EPA has regulated other VOCs under the Safe Drinking Water Act (SDWA) and amendments, the Resource Conservation and Recovery Act (RCRA) and amendments, the Clean Air Act (CAA) and amendments, and the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA or Superfund) and amendments. Although the VOCs listed in these lists are not identical to the list in Table 1, many of the compounds found on these other lists are included in Table 1, and, therefore, the results of this study are considered to be applicable to the VOCs found on these other list's. "Gases" and "Water-Soluble" Compounds Two groups of compounds present unique analytical problems in the determination of VOCs. These groups are the "gases" and "water-soluble compounds." The priority pollutant gases are chloromethane, bromometharie, chloroethane, and vinyl chloride. These gases boil below approximately 15c C and are readily lost from aqueous solutions. These losses make the analysis more variable than for compounds that are not lost as readily. Conversely, the water- soluble compounds present a separate set of analytical problems because they are not readily purged from water. In this study, the water-soluble priority pollutants tested are acrolein, acrylonitrile, and 2-chloroethylvinyl ether. Non-priority pollutant water-soluble compounds tested were acetone, 2-butanone (MEK), p-dioxane, and diethyl ether. Control of Discharges The Engineering and Analysis Division (EAD), within the Office of Science and Technology in EPA's Office of Water, is responsible for promulgating regulations controlling the discharge of pollutants to U.S. surface waters. EAD conducts surveys of the regulated industry to establish the best pollutant control strategies within various categories and subcategories September 1995 ------- Comparison of VOA Compositing Procedures (Reference 3). In these surveys, EAD frequently samples and analyzes wastewaters to determine the presence and concentration of pollutants. Although these studies primarily focus on the 126 priority pollutants (40 CFR 423, Appendix A) and the five conventional pollutants (40 CFR 401.16), other "non-conventional" pollutants may also be determined and subsequently proposed for regulation. In conducting these surveys, EPA collects aqueous samples in and around wastewater treatment plants and other locations. Unless treatment system characteristics dictate otherwise, VOA samples are composited to effect a cost savings over the analysis of individual grab samples. Normally, four individual grab samples are collected at approximately equal time intervals over the course of a 24-hour day. These samples are stored at 4 C, shipped under wet ice to the testing laboratory and composited in the laboratory. Results of these analyses are then used, in part, to develop, propose, and promulgate effluent guidelines and standards for the appropriate industrial category at 40 CFR Parts 403 - 499. September 1995 ------- Comparison of VOA Cc Theoretical Considerations and Prior Work VOA compositing is used extensively by EPA for data gathering in regulatory development programs, and is used for compliance monitoring under EPA rules. The technical literature is replete with theoretical discussions of the effects that compositing may have on data integrity Book chapters on the subject by Gilbert (Reference 4) and by Garner et al. (Reference 5) provide comprehensive evaluations of the concepts behind composite sampling and provide extensive bibliographies referencing the technical literature on sample compositing and statistical treatments of the compositing process. Although the technical literature contains many theoretical discussions of VOA compositing it is remarkably silent concerning data gathering designed to verify the theoretical work A search of online databases revealed only one technical paper that presents actual results of a VOA compositing study (Reference 6). Variability of Individual and Composite Measurements Any empirical measurement process has inherent variability, and the measurement of each analyte in each analysis is accompanied by an analytical error. This error is normally characterized by replicate measurements and is expressed as the standard deviation of the concentration or is normalized to the concentration as the "relative standard deviation" or "coefficient of variation." For example, the concentration of chloroform may be determined by purge-and-trap GC/MS with a relative standard deviation of 10 percent. The effect of measurement error on the result for a composite sample and on the average of individual grab samples can be understood most easily if it is assumed that the concentration of a pollutant is identical in all of the individual grab samples. Averaging the results for analysis of four individual samples requires determination of the concentration four separate tunes. Because measurement error is inversely proportional to the square root of the number of measurements, the measurement error associated with the four individual grab samples will be one-half of the error associated with any individual measurement. Because the determination of concentration in a composite sample is an individual measurement the error associated with the average of four individual grab samples will be one-half of'the error associated with the measurement of a composite sample. Therefore, the most precise and accurate results will be produced if the individual grab samples are analyzed and the results averaged. The cost of analysis, however, will be four times as great as the cost for analysis of a single composite sample. Similar accuracy and precision could be achieved if the compositing process were replicated four times and the four composites analyzed separately, assuming that no error occurred in the compositing procedure Pragmatic •September 1995 ------- Comparison of VOA Compositing Procedures considerations (e.g., cost and time) frequently outweigh the benefits acquired by measurement of grab samples individually; so discussions of error become moot, and the error associated with measuring concentration in a single composite sample becomes the only measurement error that must be considered. September 1995 ------- Comparison of VOA Compositing Procedures Types Of Compositing Time Compositing Time compositing is the most common type of sample compositing. Samples are collected from a fixed sampling point over some fixed period of time, usually a 24-hour period beginning at midnight. Samples can be collected as discrete grab samples at intervals throughout the fixed time period, or continuously over the period. Transients The objective of sampling over time, whether the sampling is grab or continuous, is to attempt to capture the transient nature of compounds in the waste stream. Capture of transients requires a knowledge of the flow characteristics of each individual stream: system volumes, flow rates, and the nature of the transient wave. If the objective is to capture the concentration maximum, the ideal scheme is to collect a grab sample at the apex of the wave. Unfortunately, this scheme is frequently impractical. The next best scheme is to collect samples at frequent enough intervals to assure that some fraction of the transient will be captured. Although use of a continuous compositor will assure capture of the transient, the transient may be diluted by the stream before and after the passage of the wave. Therefore, if monitoring of transients in a waste stream is necessary to characterize treatment system operation, samples should be collected over the wave to model the wave. After the wave characteristics are known, the intervals for subsequent sampling can be determined. Treatment System Detention Times For treated effluents, a common mistake made by personnel unfamiliar with treatment system operation is to require grab samples at intervals more frequent than the detention time of the treatment system. For example, if the treatment system has a detention time of 6 hours, sampling the effluent from the system more frequently than every few hours is unnecessary, particularly if the samples are analyzed individually. Spatial Compositing Samples from different sampling points can be composited in an effort to save analysis costs. If an analyte is present in a composited sample, each sampling point can then be sampled individually to determine the point or points contributing to the level of the analyte in the sample. Spatial compositing of up to five streams is allowed, at the discretion of the States, under EPA drinking water regulations to reduce the total number of samples that small drinking water treatment system operators must analyze [40 CFR 141.24(f)(14)]. However, the analytical system must be capable of detecting one-fifth of the maximum contaminant level (MCL) required for an individual sample. This requirement can usually be met by compositing five 5-mL samples and purging a 25-mL composite, as suggested in the CFR. September 1995 ------- Comparison of VOA Compositing Procedures Flow or Volume Compositing As the name implies, flow or volume compositing involves proportioning the sample according to the flow rate or volume of the stream being sampled. The most common use of flow compositing is in stormwater sampling pursuant to EPA's stormwater rules [40 CFR 122.21(g)(7); Reference 7]. These rules require that the composited sample proportionately represent the runoff that occurs in a stormwater event. Because it is impossible to know beforehand the total discharge volume during the event, individual grab samples must be collected at time intervals throughout the event, and varying volumes from these individual grab samples must be composited to reflect the flow during the entire stormwater event. The details of stormwater sampling and analysis, along with an example of the compositing associated with a stormwater discharge event, have been described by Stanko (Reference 8). Problems Unique to VOA Compositing The high vapor pressure of VOAs, and particularly of the volatile gases, makes these analytes particularly susceptible to loss through evaporation during any manipulation, including collection and compositing. Headspace During Sampling Analyte loss to the headspace of a container has been documented by Cline and Severin (Reference 6). Therefore, it is imperative that headspace be eliminated during sampling and sample shipment. In this study, the loss of volatiles was not critical because the objective was to compare the results from analyses of individual grab samples with the results from analysis of a composited sample. If the loss of VOCs from the individual grab samples and from the samples that feed the composite are the same, there is no consequence to this loss. Losses During Compositing None of the existing compositing procedures requires that compositing be performed with zero headspace, and such a system in a laboratory is difficult to envision. Because such a system does not exist, exposure of the sample to the atmosphere can result in analyte losses through evaporation. Loss can be minimized by cooling the sample and minimizing the exposure time. In this study, all compositing (except continuous compositing) was performed rapidly with the VOA vials chilled to 0 - 4 C. Compositing Procedures Definitions Sample: The water collected in a sample jug from a specific location at a specific time. Individual grab sample: An aliquot poured from the sample jug. Duplicate grab sample: A second aliquot poured from the same sample jug. 10 September 1995 ------- Comparison of VOA Compositing Procedure Replicate grab sample: Any aliquot poured from the sample jug. Composite sample: The physical combination of four grab samples collected at different times on the same day. Mathematical composite: The mathematical average of the analytical results of four individual grab samples. Manual Compositing Two types of manual compositing procedures were tested in this study: flask compositing and purge device compositing. Each of these procedures is described below. A third procedure, syringe compositing, is also described below but was not tested because of resource limitations! Flask Compositing (44 FR 69555^ In the flask compositing procedure, a 300- to 500-mL round-bottom flask is immersed in an ice bath. The individual VOA grab samples, maintained at 0 - 4°C, are slowly poured into the round-bottom flask. The flask is swirled slowly to mix the individual grab samples. After mixing, multiple aliquots of the composited sample are poured into VOA vials and sealed for subsequent analysis. An aliquot can also be poured into a syringe for immediate analysis. Purge Device Compositing [40 CFR 141.24(f)(1)(v)] Equal volumes of individual grab samples are added to a purge device to a total volume of 5 or 25 mL. The sample is then analyzed. Syringe Compositing [40 CFR 141.24(f)(14)(iv)] In the syringe compositing procedure, equal volumes of individual grab samples at a temperature of 0 - 4° C are aspirated into a 25-mL syringe while maintaining zero headspace in the syringe. Either the total volume in the syringe or an aliquot is subsequently analyzed. The disadvantage of this technique is that the individual samples must be poured carefully in an attempt to achieve equal volumes of each. An alternate procedure uses multiple 5-mL syringes that are filled with the individual grab samples and then injected sequentially into the 25-mL syringe. Automated Collection and Compositing Two types of automated equipment are available for sample collection and/or compositing. These are: (1) automated grab collection; and (2) automated continuous collection/compositing. These devices are described below. September 1995 ,, ------- Comparison of VOA Compositing Procedures Automated Grab Collection Automated grab collection can be accomplished using devices such as the Isco, Inc. Model 6000 automatic VOC sampler. With this system, a small bladder pump forces sample into a 40- mL VOA vial after rinsing the vial with three vial volumes as required by the method. In addition, the system overfills the vial to eliminate headspace. Up to 25 samples can be collected at a minimum of 5-minute and a maximum of 10-hour intervals. Samples are maintained at 0 - 4 C during collection. Automated Continuous Collection/compositing An automated system such as the Associated Design and Manufacturing Co. (ADM) automated continuous compositing system can be used to collect samples over a given sampling period. Sample is pushed into a bubble trap in the sampler via a peristaltic pump. The sample is then drawn into an air-tight glass syringe, the volume of which is controlled by a piston connected to a timer. The timer, and therefore the sampling frequency, is set by the user, or can be connected to a flow meter so that sampling frequency is dependent upon the flow rate. Upon completion of the sampling event, the syringe is capped with a Luer-Lok™ closure, and the entire syringe is delivered to the laboratory for sample analysis, thereby maintaining zero- headspace conditions. 12 September 1995 ------- Comparison of VOA Compositing Procedures Study Phases The objective of these studies was to compare the analytical results for manually collected individual grab samples to the analytical results for composited samples and automatically collected grab samples, to determine if bias occurred in the automated grab or compositing processes. Several compositing methods were investigated including: flask compositing and purge device compositing of automated and manual grab samples, and continuous compositing. The study reported here was divided into three phases: Phase I, a pilot phase with spiked reagent water and an unspiked field sample; Phase II, which used spiked field samples to compare flask or purge device compositing with mathematical compositing; and Phase III, which used spiked field samples to compare purge device compositing, automated compositing, automated grab collection, and mathematical compositing. Sample Collection, Shipment, and Storage All samples collected at industrial or municipal sites were preserved to pH < 2, refrigerated, and shipped to the laboratory under wet ice via overnight courier. If free chlorine was present in the sample, the sample was additionally preserved with sodium thiosulfate. Samples were stored in the laboratory at 0 - 4° C from the time of collection until analysis. All analyses were performed within the method-specified 14-day holding time. Phase I and Phase II samples were collected by passage of a portion of the flowing sample stream through a coil of pre-cleaned polytetrafluoroethylene (PTFE) tubing that was immersed in a commercial picnic cooler filled with ice. This practice reduced the temperature of the sample to 0-4° C, thus reducing the volatility of the VOCs. The stream from the PTFE tubing was collected in a cooled one-liter glass jug. Samples were preserved to pH < 2 in this jug and free chlorine was removed, as required, using sodium thiosulfate. After preservation, samples were allocated from the one-liter jug into 40-mL VOA vials. Vials were filled from the common jug, thus assuring that each replicate VOA vial in the set contained identical samples. The vials were filled to overflowing, then capped with a PTFE- faced silicone rubber septum. After capping, each VOA vial was inverted and inspected for an air bubble. If a bubble was present, the vial was uncapped and refilled to overflowing and re- capped until completely filled without an air bubble. Each vial was assigned a unique sample number. Sampling times were at approximately 9 a.m., noon, 3 p.m., and 6 p.m. Collection procedures for Phase III sampling are outlined in the Phase III study design section. Analyses All laboratory analyses were performed by Pacific Analytical, Inc., in Carlsbad, California. A single laboratory was chosen to perform this work because EPA desired to minimize analytical variability in order to increase the probability that differences between grab sampling and compositing procedures would be detected. September 1995 10 ------- Comparison ofVOA Compositing Procedures Calibration All analyses were performed by isotope dilution GC/MS using Revision C of EPA Method 1624. Revision C is an updated version of the method promulgated for use in water programs (40 CFR 136, Appendix A). Revision C includes a "reverse-search" technique for identification and quantitation of pollutants other than the priority pollutants. In the promulgated version and in Revision C of Method 1624, the priority pollutants and certain additional compounds are determined using a 5-point calibration for quantitation. Nominal calibration points are 10, 20, 50, 100, and 200 ug/L. In addition, the list of "reverse search" compounds is determined from relative retention time data and response factors given in the method. In this study, the method of quantitation was examined in relation to recovery of the VOCs for which the instrument was calibrated. The calibration procedures in Method 1624 require use of an average relative response or a calibration curve for isotope dilution calibration based on the five calibration points. However, because the analytes were spiked at known concentrations, it is possible to use the calibration point closest to each known concentration for calibration. This technique was used for calculation of all concentrations in Phases II and III of this study and reduced the analytical error to less than that obtained using the average of the five calibration points or a calibration curve. This practice of using the closest calibration point should only be employed when the concentration of a pollutant in a sample is known to be close to the calibration point. For samples containing unknown concentrations, such as the unspiked field samples in Phase I, the most accurate analyte concentrations will be found using the entire 5-point calibration curve. Data Processing and Reporting Data were received by the EPA Sample Control Center in the form of quantitation reports on diskette. These data included quality control (QC) data for each analysis. The QC data included recoveries for each labeled compound spiked. The QC data were tested against the QC acceptance criteria in the method using a modified version of QA Formaster™ supplied by Thermo-Finnigan Corp. Non-compliant data were resolved with the laboratory. 14 September 1995 ------- Comparison of VOA Compositing Procedures Phase I Study Design Two types of samples were analyzed in Phase I: an unspiked field sample set, and two spiked reagent water sample sets. Each set consisted of four aliquots representing a single sampling point and time. Reagent water was used to test compositing effects in the absence of matrix effects, and to eliminate any possible interference from native pollutants. Two concentrations were used to test whether compositing effects were concentration dependent. In order to ensure complete solubility, each of the four aliquots per set was spiked with one- fourth of the total analyte list at either 100 ug/L or 600 ug/L , resulting in composite samples with nominal concentrations of 25 ug/L and 150 ug/L , respectively. However, the 600 ug/L individual aliquots exceeded the calibration range of the instrument, and so these aliquots were diluted 1:3 (sample:reagent water) prior to analysis. The low concentration spiking scheme for each aliquot is shown in Table 2. The analyte list was separated into the four groups on the basis of solubility in water. After spiking, each aliquot was then split: one split to be physically composited with splits from each of the other three aliquots, and one split to be analyzed separately. Each analyte was present at full concentration in one of the four aliqouts, and not present in the other three. This results in the analyte being present at the nominal concentration in the composite. The results of the four individually analyzed splits were than averaged to derive the mathematical composite value. If there were 100% recovery of the spike in the individually analyzed splits, the average concentration of each analyte would be iOQug/L+OMg/L + Oug/L for the low concentration samples. Table 2 Phase I VOC Spiking Groups* Analyte Carbon tetrachloride Chlorobenzene trans-1 ,3-Dichloropropene 1 ,2-Dichloroethane Ethylbenzene Tetrachloroethene Cone. In Aliquot 1 100pg/L 100 ug/L 100 ug/l_ 100 ug/l_ loOpg/L lOOpg/L Cone. In Aliquot 2 0 0 0 0 0 0 Cone. In Aliquot 3 0 0 0 0 0 0 Cone. In Aliquot 4 0 0 - 0 0 0 0 Cone. In Composite 25 ug/L 25 ug/L 25 ug/L 25 ug/L 25 ug/L 25 ug/L September 1995 15 ------- Comparison of VOA Compositing Procedures Analyte 1 ,1 ,2-Trichloroethane 1,1-Dichloroethene trans-1 ,2-Dichloroethene 1 ,2-Dichloropropane Benzene Bromodichloromethane Bromoform Dibromochloromethane 1 ,1 ,2,2-Tetrachloroethane Toluene 1,1,1 -Trichloroethane Trichloroethene 1,1-Dichloroethane Methylene Chloride Bromomethane Chloroethane Chloromethane Vinyl chloride Diethyl ether Chloroform Acetone Acrolein Acrylonitrile 2-Butanone p-Dioxane Cone. In Aliquot 1 100 Mg/L 100 Mg/L 100|jg/L 100 Mg/L 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Cone. In Aliquot 2 0 0 0 0 100 Mg/L 100 Mg/L 100 Mg/L 100 Mg/L 100 Mg/L 100 Mg/L 100 Mg/L 100 Mg/L 100 Mg/L 100 Mg/L 0 0 0 0 0 0 0 0 0 I 0 0 Cone. In Aliquot 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 Mg/L 100 Mg/L 100 Mg/L 100 Mg/L 100 Mg/L 100 Mg/L 0 0 0 0 0 Cone. In Aliquot 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 100 }ig/L 100 Mg/L 100 Mg/L 100 Mg/L 100 Mg/L Cone. In Composite 25 Mg/L 25 Mg/L 25 ug/l_ 25 ug/l_ 25 ug/l_ 25 pg/L 25 ug/L 25 Mg/L 25 pg/L 25 Mg/L 25 Mg/L 25 Mg/L 25 Mg/L 25 Mg/L 25 Mg/L 25 Mg/L 25 Mg/L 25 Mg/L 25 Mg/L 25 Mg/L 25 Mg/L 25 Mg/L 25 Mg/L 25 Mg/L 25 Mg/L *For the high concentration group, each compound was spiked at 600 Mg/L in the same pattern. Composite concentrations were 150 Mg/L in each analyte. 16 September 1995 ------- Comparison of VOA Compositing Procedu Statistical Analyses and Results Spiked Reagent Water Samples When averaged across all compounds, the physical composite of the low concentration splits had 8% higher recoveries1 than the mathematical composite of the low concentration splits. The physical composite of the high concentration splits had 17% higher recoveries than the mathematical composite of the high concentration splits. Because there was only one sample at each concentration, statistical analyses could not be performed. As both concentrations behaved similarly, their results were combined to allow statistical analysis, and paired t tests were performed for each analyte. Of the 29 analytes for which reliable data was received, a significant difference between the mathematical composite and the physical composite was seen for only one analyte. This result would be expected on the basis of chance alone, and therefore, the results of the physical and mathematical composites are not statistically different. Unspiked Field Samples The physically composited sample had analyte concentrations that were 13% lower than those for the mathematically composited sample. This result is the opposite of that seen in the reagent water sample. However, due to the fact that there was only one unspiked field sample, no statistical analyses could be performed, and therefore, the results cannot be considered statistically significant. Despite the attempt to find an industrial source with high levels of volatiles, only 10 analytes were present in measurable quantities. This lead to the decision to spike field samples in future studies. throughout this document, the term "recovery" refers to the percent of spike value following correction for labelled compound recovery. September 1995 , ~ ------- Comparison of VOA Compositing Procedures Phase II Study Design Four samples were collected at different times over the course of a day from seven "real- world" sites. These sites are described in Table 3. Information about each site was recorded in an on-site log and included the EPA sample number, collection date and time, descriptions of sample and sampling location, sample pH and temperature, and preservatives used, if any. Table 3 Description of Sites and Samples Episode 4559 4561 4563 4573 4575 4593 4595 Industrial Category Organic Chemicals Organic Chemicals Drum Reconditioning Shore Reception Transportation Transportation MSW Landfill Sampling Point Primary Effluent Primary Effluent Scrubber Water Oily Wastewater Separator Effluent Separator Effluent Leachate PH 8.8 7.3 8.6 5.6 6.0 6.8 6.8 Sample sites were selected based on the expectation that the effluents would contain volatile organics. However, volatile organics were seldom found and, therefore, samples were spiked with VOAs. All spiking solutions were prepared in the laboratory and all spiking was performed in the laboratory. Samples from three episodes were flask composited and samples from four episodes were purge device composited. Schematic diagrams of the flask and purge device compositing procedures are shown in Figures 1 and 2, respectively. For the episodes that were flask composited, the grab sample from the first sample time was analyzed unspiked to determine the background concentrations of VOCs present. For the episodes that were purge device composited, the grab samples from all four time points were analyzed unspiked to determine the background concentrations present. This testing was done to determine the constancy of the background throughout the sampling period, and to remove the influence of background levels from statistical analyses. Individual grab sample VOA vials from each of the four time points were spiked at concentrations of 20, 40, 80, and 40 ug/L, respectively, to produce an average concentration of 45 ug/L. An aliquot from these spiked VOA vials was analyzed and two other aliquots were used to make duplicate composites, thus assuring that the spike levels were identical for analyses of the individual and composited samples. 18 September 1995 ------- Comparison ofVOA Compositing Procedure T2 T3 T4 Key a Unspiked S 20 pg/L Spike m 40 Mg/L Spike • 80 MQ/L Spike Figure 1. Flask Compositing Scheme T1 T2 T3 T4 D D D D Key D Unspiked Q 20 M9/L Spike 0 40 |jg/L Spike • 80 pg/L Spike Figure 2. Purge Device Compositing Scheme September 1995 19 ------- Comparison of VOA Compositing Procedures Statistical Analyses And Results Analytes Tested Data were evaluated with respect to QC requirements and three analytes were dropped from further analysis due to poor quantitation: 1,1,1-trichloroethane, 2-chloroethylvinyl ether, and trans-l-2-dichloroethene. All other analytes met QC requirements and were included in all statistical analyses. Background Subtraction The background level determined from the single, unspiked sample in each of the flask- composited episodes was subtracted from the result of all grab and composite samples for that episode. For each grab sample in the purge device-composited episode, the background level from the sample collected at the same point and time was subtracted from the analytical result. For each composite sample, the results of the four individual backgrounds were averaged, and the resulting value was subtracted from the composite results. Statistical Analyses For each analyte in each episode, the percent recoveries in the four grab samples were averaged, as were those of the two physical composites. The median recovery across all analytes and episodes was calculated. In addition, the ratio of mathematical composite recovery to physical composite recovery was calculated for each analyte in each episode, according to the formula ^ t. Mean mathematical composite recovery Ratio = ;—• Mean physical composite recovery A two-tailed Student's t test was performed to determine if this ratio was significantly different from 1.0, at the 5% level. In addition, a two-tailed Student's t-test was performed to determine if there were any differences between recoveries in samples composited in a flask and recoveries in samples composited in the purge device. When the number of samples in the two groups being compared are unequal, the Student's t test results are affected more severely by unequal variances for the two groups (Reference 10, p. 230). Since the variance for flask- composited samples was unequal to that for purge device-composited samples, and the sample sizes differed between the two groups, Satterthwaite's correction for unequal variances was applied to the t test calculations. Results The pollutants detected in the real-world samples were mainly the water-soluble compounds, resulting in high analytical variability. Statistically significant differences between the mathematically averaged results from analysis of the individual grab samples and the result from analysis of the physically composited sample were identified for a number of analytes. However, the size of the difference is small and may not be meaningful when compared to the 2Q September 1995 ------- Comparison of VOA Compositing Procedures analytical variability. The two compositing methods, flask and purge device, provide analytical results for all analytes that are not statistically different. Comparison of Grab and Composite Results Table 4 compares composite recovery with grab recovery. These tests show that, for the analytes for which background subtraction was not required and from which the gases and water-soluble compounds were excluded, the median result for grab samples was 12.2 percent higher than the median result for the flask composites, and 7.3 percent higher than the median result for the purge device composites. Depending on the analyte and analytical conditions, between 16% and 62% of the analytes show significantly lower concentrations in the composite samples than in the individual grab samples. The number of analytes showing these effects is much greater than would be expected by chance alone. In addition, the two compositing procedures show results in the same direction. The size of the effect, as a percent of the grab concentration, ranges up to 21, with the median across analytes showing the effect in the range of 6-13 percent. However, for routine VOA analyses this effect may not be significant compared to other sources of analytical variability. Flask and Purge Device Compositing Two-tailed Student's t-tests were used to determine the significance of differences between the two techniques for each analyte. Only 1 out of 20 analytes showed a significant effect on a two-tailed test at p=0.05; this could easily be due to chance variation. The two compositing procedures, therefore, give consistent results. Analytes tested were those with data for at least 3 samples by each method. September 1995 21 ------- Comparison ofVOA Compositing Procedures Table 4 Comparison of Composite Recovery with Grab Recovery Composite Location Flask Purge Device Background Excluded Subtracted Excluded Subtracted Gas/HoO Solubfe No Yes No Yes No Yes No Yes Analytes Tested 26 11 23 9 25 11 25 11 Number Signif. 16 5 12 4 9 5 4 5 Median Effect* 12.2% 13.0% 12.3% 13.1% 7.3% 9.0% 6.2% 9.3% Max. Effect* 21.0% 16.1% 21.0% 16.1% 12.5% 15.3% 11.1% 15.3% * Effect = 100 x (grab concentration - composite concentration)/grab concentration. "Background" indicates whether analytes present prior to spiking were background-subtracted or excluded from the analysis. Analytes tested were those with data for at least 3 samples; significance was tested at the p=0.05 level, two-tailed. As stated above, all significant differences were in the direction of lower composite concentration. 22 September 1995 ------- Comparison ofVOA Compositing Procedures Phase Study Design The purpose of this study phase was to compare manual grab, automated grab, mathematical compositing and automated compositing techniques. Two sites, with markedly different effluent matrices, were chosen for sampling: a POTW and a bus maintenance facility. Effluent from each site was collected, well mixed, and divided among four collapsible bags. Collapsible bags were used to minimize the amount of head space created as samples were withdrawn from the bags. Each bag was spiked with a different level of VOCs to simulate collection at different times. The spiking levels used were the same as those used in Phase II. Samples collected by all techniques at a single time were drawn from the same bag. The automated composite was drawn for the same length of time from each of the four bags. The sampling scheme for Phase III is shown in Table 5 and Figure 3. From each bag, two grab samples were drawn manually and one drawn with an automated sampler. In addition, one sample from each site was drawn by an automated compositor. The automated compositor was set to draw a 0.3 mL sample every 40 seconds, until a total of approximately 10 mL was drawn (34 samples over a 23 minute period). This procedure was repeated in each of the four bags. One sample from each manually-drawn pair was assigned to the group to be analyzed directly and mathematically composited. The second manual grab sample was assigned to the group to be physically composited. Another single sample from each of the four bags was manually drawn prior to spiking, and was analyzed individually to assess the background levels of analytes present in the samples. Phase Table 5 Sampling Scheme Bag (Time) 1 2 3 4 Per Site Samples Manual Grab Manual Grab Manual Grab Manual Grab Mathematically Composited Automated Grab Automated Grab Automated Grab Automated Grab Mathematically Composited Manual Grab Manual Grab Manual Grab Manual Grab Physically Composited Back- ground Back- ground Back- ground Back- ground Automated Composite September 1995 23 ------- Comparison ofVOA Compositing Procedures E33Manual Grab HI Automated Grab iBManual Composite HH Automated Composite O Unspiked © 20 ug/L Spike 40 ug/L Spike 80 ug/L Spike Figure 3. Phase III Compositing Scheme Description of Sampling Equipment Automated Grab Sampler Automated grab samples were collected using an automated VGA sampler (Model 6000, Isco Environmental Division, Lincoln, NE). Sample is collected via a bladder pump that pushes the sample into the vial and does not expose the sample to a vacuum or partial vacuum. Prior to collection, the Model 6000 rinses the sample line and overfills the VGA vial three times, as required by the method and to eliminate headspace. The vials are filled via a syringe needle in a 360 stream designed to remove any air bubbles that may cling to the vials. The vials are covered with caps containing an air-tight valve that is opened for filling. When a vial is filled, the valve is closed. Automated composite samples were collected using an automated VGA compositing sampler (AVOCS®-500, Associated Design and Manufacturing Co., Alexandria, VA). Sample is pushed into a bubble trap in the sampler via a peristaltic pump. The sample is then drawn into an air- 24 September 1995 ------- Comparison ofVOA Compositing Procedures tight glass syringe, the volume of which is controlled by a piston connected to a timer. The timer, and therefore the sampling frequency, is set by the user, or can be connected to a flow meter so that sampling frequency can be coupled to the flow rate. Upon completion of the sampling event, the syringe is capped with a Luer-Lok™ closure, and the entire syringe is delivered to the laboratory for sample analysis, thereby maintaining zero-headspace conditions. Statistical Analyses and Results Statistical Analyses Analyte concentrations detected at each sampling time (bag) were background-corrected using the concentrations found in the background sample for that time. For the automated composite, the background value used for correction was the average of the background values for each bag. For each analyte in each episode, the percent of spike recoveries in the manual grab samples were averaged, as were those of the automated grab samples. A Dunnett's test was performed using the mathematical composite of the manual grab as the control group. This test allows comparison of multiple techniques to a single control group. In addition, for each sampling technique, median and maximum effects were calculated. As in Phase II, effects are in terms of the difference between grab recovery and recovery by a particular technique, as a percentage of the grab recovery. Results The results are summarized in Table 6. For 11 of 30 analytes (37%), the physically composited samples had significantly lower recoveries than the mathematical composite of the manual grab samples. This percentage is far more than one would expect based on chance alone. Neither the automated compositor nor the automated sampler produced results which were statistically different from the mathematical composite of the manual grab sample for any analyte. Table 6 Comparison of Alternative Sampling Techniques Sampling Technique Automated Grab Automated Composite Physical Composite Number of Analytes 30 30 30 Number Significantly Different 0 0 11 Median Effect* /o/ \ (/o) -4.6 8.1 15.0 Maximum Effect* (%) -29.8 43.8 -42.0 * Effect = 100 x (Manual grab - technique) / Manual grab; a negative effect indicates that the technique resulted in higher recoveries than the manual grab. September 1995 25 ------- ------- Comparison of VOA Compositing Procedures Conclusions and Discussion Mathematical averages of the results from analyses of grab samples were found to be larger than the result from the analysis of either flask- or purge device-composited samples, although these differences are on the order of a few percent and would not be discernable except by isotope dilution quantitation procedures. In addition, the number of samples tested in this study (from 1 to 7, depending on the phase) was relatively small, even though the number of analytes per sample (29-40) was large. Because the behavior of one analyte can be expected to be correlated with that of other analytes in the sample, it is possible that the small number of samples results in differences that would be negated or lost in a larger study. For example, it is possible that the matrix for a particular sample contributed to the loss of analytes during compositing. If the behavior of analytes is correlated, then similar losses would be expected for many analytes in that sample. Because the primary metric in this study was the number of analytes that showed significant loss, a large difference in one sample out of the small number of samples could have a large impact on the study results. However, it is not know whether matrices have a differential effect on analyte loss. The recoveries for the composited samples using reagent water in Phase I were greater than the average of the non-composited samples; in Phases II and III, the composited samples produced recoveries lower than the average of the non-composited samples. The reasons for these differences among the study phases are not known, but there are several possibilities. First, the results of the Phase I reagent water were not statistically significant, in part due to the small number of samples. Second, the individual reagent water aliquots were diluted 1:3 (sample:reagent water) while the composited reagent water aliquots were not. It is possible that some loss of analytes occurred during the dilution procedure. Third, it is possible that field samples have a greater loss of analyte during compositing due to the effects of the matrix or to interference by native analytes. Last, it is possible that the differences are due to measuring or compositing errors, even though calibrated syringes and volumetric glassware were used. Compositing can be useful in some situations and will result in a cost savings over the analysis of individual grab samples. However, compositing may introduce some small systematic error in the analytical results. EPA plans to continue the use of VOA compositing in its effluent guidelines program and, after further studies, may promulgate compositing procedures for wastewaters. September 1995 07 ------- ------- Comparison of VOA Compositing Procedures References 1. Keith, L.H. and Telliard, W.A. Env. Sci. & Tech.. 1979, 13(4) 416 - 423. 2. Bellar, T.A., Lichtenberg, J.J., and Kroner,R.C. J. Am. Water Works Assoc.. 1974, 66, 703 - 706. 3. Telliard, W.A., Rubin, M.B., and Rushneck, D.R. J. Chromatog. Sci.. 1987, 25, 322 - 327. 4. "Compositing and Three-Stage Sampling", from Statistical Methods for Environmental Monitoring. Gilbert, R.O. Editor, D. Van Nostrand, New York, 1987. 5. Garner, F.C., Stapanian, M.A., and Williams, L.R., "Composite Sampling for Environmental Monitoring", from Principles of Environmental Sampling. Keith, L.H. Editor, American Chemical Society, Washington DC, 1988. 6. Cline, S.M., and Severiri, B.F. Water Res.. 1989, 23(4) 407 - 412. 7. EPA 833-B-92-001, 1992 8. Stanko,G.H. Environmental Lab. 1994, 6(2) 10 - 15. 9. Hoaglin, D.C., Mosteller, F., and Tukey, J.S. Understanding Robust and Exploratory Data Analysis. John Wiley and Sons, New York, 1983. 10. Snedecor, G.W. and Cochran, W.G. Statistical Methods (eighth edition). Iowa State University Press, Ames, 1989. September 1995 29 ------- ------- Appendix A Phase I Data ------- ------- Comparison of VOA Compositing Procedures Phase I - Unspiked Field Sample Data Analyte Concentration (jag/L) ANALYTE 2-BUTANONE 2-PROPANONE 4-METHYL-2 -PENTANONE CHLOROFORM ETHYLBENZENE ISOBUTYL ALCOHOL M-XYLENE 0+P XYLENE TETRACHLOROETHENE TOLUENE TRICHLOROFLUOROMETHANE Mathematical Composite 357.14 10744.84 105.78 180.08 22.74 26.30 41.82 29.94 45.82 181.85 32.90 Physical Composite 178.53 7459.43 67.03 161.71 21.21 47.19 31.77 41.70 161.41 34.40 September 1995 A-l ------- Comparison of VOA Compositing Procedures Phase I - Spiked Reagent Water Data Percent Recovery of Spikes . , iiicfn C. oncent ration • Mathematical ANALYTE NAME 1,1, 1-TRICHLOROETHANE 1,1,2,2 -TETRACHLOROETHANE 1,1,2 -TRICHLOROETHANE 1 , 1-DICHLOROETHENE 1 , 2-DICHLOROETHANE 1 , 2-DICHLOROPROPANE 1,4-DIOXANE 2-BUTANONE 2-PROPANONE 2 -PRO PENAL ACRYLONITRILE BENZENE BROMODICHLOROMETHANE BROMOMETHANE CHLOROBENZENE CHLOROETHANE CHLOROFORM CHLOROMETHANE DIBROMOCHLOROMETHANE DIETHYL ETHER ETHYLBENZENE METHYLENE CHLORIDE TETRACHLOROETHENE TETRACHLOROMETHANE TOLUENE TRANS- 1, 2-DICHLOROETHENE TRANS- 1 , 3-DICHLOROPROPENE TRICHLOROETHENE VINYL CHLORIDE Composite 65.090 186.322 101.457 33.839 74.214 90.028 70.086 68.824 66.402 56.863 93.371 63.462 83.349 60.393 74.594 48. 000 78.140 51.208 87.038 86.957 66.712 79.693 63.577 48.719 76.557 36.805 8.392 86.775 38.895 Physical Composite 84.630 104.438 112.564 71.735 81.336 101.859 98.706 82.143 82.180 65.999 92.072 78.418 97.070 58.326 86.913 38.372 71.504 38.725 96.361 84.197 87.389 97.755 78.527 96.309 94.174 50.857 7.890 120.956 21.150 A-2 September 1995 ------- Comparison of VOA Compositing Procedures Phase I - Spiked Reagent Water Data Percent Recovery of Spikes ANALYTE NAME 1,1,1-TRICHLOROETHANE 1,1,2,2-TETRACHLOROETHANE 1,1,2-TRICHLOROETHANE 1,1-DICHLOROETHENE 1,2-DICHLOROETHANE 1,2-DICHLOROPROPANE 1,4-DIOXANE 2-BUTANONE 2-PROPANONE 2-PROPENAL ACRYLONITRILE BENZENE BROMODICHLOROMETHANE BROMOMETHANE CHLOROBENZENE CHLOROETHANE CHLOROFORM CHLOROMETHANE DIBROMOCHLOROMETHANE DIETHYL ETHER ETHYLBENZENE METHYLENE CHLORIDE TETRACHLOROETHENE TETRACHLOROMETHANE TOLUENE TRANS-1,2-DICHLOROETHENE TRANS-1,3-DICHLOROPROPENE TRICHLOROETHENE VINYL CHLORIDE . en u J. a u _Li_iii Mathematical Composite 33.288 167.883 106.369 45.451 77.358 91.312 76.002 80.217 90.481 64.039 99.959 48.498 80.002 50.303 78.741 35.325 69.5035 38.9250 82.1605 85.3305 74.1770 72.8565 70.4925 63.4920 57.1250 39.6740 10.7310 54.9185 23.0345 Physical Composite 65 127 124 57 86 101 159 98 159 62 144 70 105 55 89 41 74 38 114 85 84 109 80 71 82 51 96 21 .347 .389 .057 .013 .549 .143 .430 .540 .593 .613 .573 .288 .830 .540 .123 .987 .761 .700 .159 .413 .400 .240 .151 .291 .931 .291 .264 .433 September 1995 A-3 ------- ------- Appendix B Phase II Data ------- ------- Comparison of VOA Compositing Procedures Phase II Data - Percent of Spike Recoveries JT -L d. o JS. ^ CJiUpCJ t> _L 1 — LI1CJ Mathematical EPISODE 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 ANALYTE 1,1,2, 2 -TETRACHLOROETHANE 1,1,2, 2 -TETRACHLOROETHANE 1,1,2, 2 -TETPACHLOROETHANE 1,1,2 -TRICHLOROETHANE 1 , 1 , 2 -TRICHLOROETHANE 1,1, 2 -TRICHLOROETHANE 1, 1-DICHLOROETHANE 1, 1-DICHLOROETHANE 1, 1-DICHLOROETHANE 1, 1-DICHLOROETHENE 1 , 1-DICHLOROETHENE 1 , 1-DICHLOROETHENE 1 , 2 -DICHLOROETHANE 1 , 2 -DICHLOROETHANE 1 , 2 -DICHLOROETHANE 1 , 2 -DICHLOROPROPANE 1 , 2 -DICHLOROPROPANE 1 , 2 -DICHLOROPROPANE 2-HEXANONE 2-HEXANONE 2-HEXANONE 4 -METHYL- 2 - PENTANONE 4 -METHYL- 2 - PENTANONE 4 -METHYL- 2 - PENTANONE ACETONE ACETONE ACETONE ACROLEIN ACROLEIN ACROLEIN ACRYLONITRILE ACRYLONITRILE ACRYLONITRILE BENZENE BENZENE BENZENE BROMODICHLOROMETHANE BROMODICHLOROMETHANE BROMODICHLOROMETHANE BROMOFORM BROMOFORM BROMOFORM BROMOMETHANE BROMOMETHANE BROMOMETHANE Composite 99 104 102 103 105 103 108 106 103 124 101 100 100 102 100 106 104 99 1003 351 295 198 184 883 356 386 821 52 67 66 108 95 96 160 105 103 105 109 102 105 136 103 179 105 101 .4 .2 .8 .4 .9 .5 .4 .0 .6 .8 .9 .8 .3 .4 .6 .3 .9 .6 .2 .3 .1 .3 .8 .5 .6 .7 .6 .8 .3 .6 .1 .5 .0 .4 .0 .5 .7 .2 .8 .8 .5 .2 .7 .5 .8 Physical Composite 109 102 90 109 102 96 101 95 91 113 90 86 101 99 92 101 99 91 987 311 266 199 173 781 371 418 668 65 76 64 120 104 100 130 93 90 107 103 91 105 127 98 171 91 86 .2 .0 .9 .6 .6 .1 .4 .9 .9 .7 .9 .7 .9 .9 .5 .0 .0 .4 .2 .3 .0 .9 .3 .3 .3 .1 .1 .5 .2 .4 .7 .2 .2 .5 .7 .5 .6 .8 .2 .8 .9 .7 .8 .3 .2 B-l September 1995 ------- Comparison ofVOA Compositing Procedures Phase II Data - Percent of Spike Recoveries EPISODE 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 4561 4563 4559 rj-dojs. ^uun_jus> j- u -Liiy \^^u ANALYTE CARBON DISULFIDE CARBON DISULFIDE CARBON DISULFIDE CARBON TETRACHLORIDE CARBON TETRACHLORIDE CARBON TETRACHLORIDE CHLOROBENZENE CHLOROBENZENE CHLOROBENZENE CHLOROETHANE CHLOROETHANE CHLOROETHANE CHLOROFORM CHLOROFORM CHLOROFORM CHLOROMETHANE CHLOROMETHANE CHLOROMETHANE CIS-1, 3-DICHLOROPROPENE CIS-1, 3-DICHLOROPROPENE CIS-1, 3-DICHLOROPROPENE DIBROMOCHLOROMETHANE DIBROMOCHLOROMETHANE DIBROMOCHLOROMETHANE DIETHYL ETHER DIETHYL ETHER DIETHYL ETHER ETHYL BENZENE ETHYL BENZENE ETHYL BENZENE M-XYLENE M-XYLENE M-XYLENE METHYL ETHYL KETONE METHYL ETHYL KETONE METHYL ETHYL KETONE METHYLENE CHLORIDE METHYLENE CHLORIDE METHYLENE CHLORIDE 0- + P-XYLENE O- + P-XYLENE 0- + P-XYLENE P-DIOXANE P-DIOXANE P-DIOXANE TETRACHLOROETHENE • i- ; Mathematical Composite 284.3 258.3 254.6 116.0 98.8 97.5 105.5 224.2 105.4 137.0 99.9 100.9 123.3 108.1 103.6 302.3 107.7 100.1 331.1 307.8 306.4 112.5 114.0 105.6 107.1 101.5 99.8 106.0 199.1 163.1 65.3 363.9 137.0 100.7 103.3 184.5 120.2 98.1 140.3 73.7 213.6 84.5 79.9 96.6 373 .0 115.0 Physical Composite 251.8 226.4 198.0 101.1 92.6 78.6 100.3 183.1 94.6 116.3 86.9 82.9 116.0 99.4 88.1 286.6 93.5 84.5 336.9 298.9 261.6 112.2 108.7 97.0 110.4 101.5 93 .5 98.8 170.6 129.0 62.3 279.3 123.2 104.2 109.1 157.5 114.4 91.7 121.6 70.9 178.8 79.3 76.3 89.3 214.5 102.2 September 1995 B-2 ------- Comparison of VOA Compositing Procedures Phase II Data - Percent of Spike Recoveries Flask Compositing (Cont') EPISODE ANALYTE 4561 TETRACHLOROETHENE 4563 TETRACHLOROETHENE 4559 TOLUENE 4561 TOLUENE 4563 TOLUENE 4559 TRANS-1,3-DICHLOROPROPENE 4561 TRANS-1,3-DICHLOROPROPENE 4563 TRANS-1,3-DICHLOROPROPENE 4559 TRICHLOROETHENE 4561 TRICHLOROETHENE 4563 TRICHLOROETHENE 4559 TRICHLOROFLUOROMETHANE 4561 TRICHLOROFLUOROMETHANE 4563 TRICHLOROFLUOROMETHANE 4559 VINYL CHLORIDE 4561 VINYL CHLORIDE 4563 VINYL CHLORIDE Mathematical Composite 108.0 105.6 110.3 104.7 121.6 102.0 93.8 95.6 118.6 163.2 105.4 77.5 63 .2 59.1 210.1 105.2 99.0 Physical Composite 99.7 95.3 102.6 96.0 103.9 101.0 84.6 81.1 109.9 144.4 93.3 65.7 54.5 47.8 187.0 93.0 85.7 B-3 September 1995 ------- Comparison ofVOA Compositing Procedures Phase II Data - Percent of Spike Recoveries Purcfs Device Compositing — Mathematical EPISODE 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 ANALYTE 1,1,2, 2 -TETRACHLOROETHANE 1,1,2, 2 -TETRACHLOROETHANE 1,1,2, 2 -TETRACHLOROETHANE 1,1,2, 2 -TETRACHLOROETHANE 1,1, 2 -TRICHLOROETHANE 1,1, 2 -TRICHLOROETHANE 1,1, 2 -TRICHLOROETHANE 1,1,2 -TRICHLOROETHANE 1 , 1-DICHLOROETHANE 1, 1-DICHLOROETHANE 1 , 1-DICHLOROETHANE 1 , 1-DICHLOROETHANE 1 , 1-DICHLOROETHENE 1 , 1-DICHLOROETHENE 1 , 1-DICHLOROETHENE 1 , 1-DICHLOROETHENE 1 , 2 -DICHLOROETHANE 1 , 2 -DICHLOROETHANE 1 , 2 -DICHLOROETHANE 1 , 2 -DICHLOROETHANE 1 , 2 -DICHLOROPROPANE 1 , 2 -DICHLOROPROPANE 1 , 2 -DICHLOROPROPANE 1 , 2 -DICHLOROPROPANE 2-HEXANONE 2-HEXANONE 2-HEXANONE 2-HEXANONE 4 -METHYL- 2 - PENTANONE 4 -METHYL- 2 - PENTANONE 4 -METHYL- 2 -PENTANONE 4 -METHYL- 2 -PENTANONE ACETONE ACETONE ACETONE ACETONE ACROLEIN ACROLEIN ACROLEIN ACROLEIN ACRYLONITRILE ACRYLONITRILE ACRYLONITRILE ACRYLONITRILE BENZENE BENZENE Composite 91 97 116 99 97 90 101 89 97 92 98 108 98 88 96 92 96 91 98 97 94 93 102 94 341 316 457 330 205 167 263 169 68 135 79 58 63 16 23 140 103 106 111 98 90 91 .5 .9 .6 .2 .5 .7 .2 .8 .1 .0 .7 .4 .3 .7 .8 .4 .3 .9 .4 .2 .5 .2 .6 .9 .9 .6 .8 .6 .5 .4 .6 .6 .2 .2 .2 .0 .5 .2 .3 .7 .4 .0 .3 .3 .5 .7 Physical Composite 94 99 117 94 85 88 93 81 79 86 90 89 77 79 87 76 83 87 92 85 81 90 95 83 301 317 423 316 182 159 229 170 62 120 90 44 71 9 12 5 96 108 103 98 73 83 .4 .6 .6 .4 .1 .6 .7 .7 .3 .4 .4 .8 .1 .2 .0 .2 .0 .9 .4 .2 .0 .7 .8 .8 .8 .1 .7 .6 .6 .1 .2 .4 .3 .5 .8 .6 .9 .6 .2 .2 .2 .0 .9 .8 .0 . 6 September 1995 B-4 ------- Comparison ofVOA Compositing Procedures EPISODE 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 Phase II Data - Percent of Spike Purge Device Compositincj ANALYTE BENZENE BENZENE BROMODICHLOROMETHANE BROMODICHLOROMETHANE BROMODICHLOROMETHANE BROMODICHLOROMETHANE BROMOFORM BROMOFORM BROMOFORM BROMOFORM BROMOMETHANE BROMOMETHANE BROMOMETHANE BROMOMETHANE CARBON BISULFIDE CARBON DISULFIDE CARBON DISULFIDE CARBON DISULFIDE CARBON TETRACHLORIDE CARBON TETRACHLORIDE CARBON TETRACHLORIDE CARBON TETRACHLORIDE CHLOROBENZENE CHLOROBENZENE CHLOROBENZENE CHLOROBENZENE CHLOROETHANE CHLOROETHANE CHLOROETHANE CHLOROETHANE CHLOROFORM CHLOROFORM CHLOROFORM CHLOROFORM CHLOROMETHANE CHLOROMETHANE CHLOROMETHANE CHLOROMETHANE CIS-1, 3-DICHLOROPROPENE CIS-1, 3-DICHLOROPROPENE CIS-1, 3-DICHLOROPROPENE CIS-1, 3-DICHLOROPROPENE DIBROMOCHLOROMETHANE DIBROMOCHLOROMETHANE DIBROMOCHLOROMETHANE DIBROMOCHLOROMETHANE ; Recover: (Cont ' ) Mathemat: ies ical Composite 98 90 92 92 97 92 99 98 101 113 97 94 110 101 240 217 241 225 101 94 99 198 115 94 131 95 98 88 96 104 97 83 103 91 102 95 105 101 317 280 292 296 96 89 97 97 .9 .4 .8 .6 .0 .1 .0 .7 .2 .3 .3 .7 .2 .2 .2 .7 .4 .0 .1 .9 .5 .6 .3 .4 .5 .9 .7 .2 .0 .5 .1 .9 .0 .2 .6 .6 .0 .4 .8 .0 .4 .0 .2 .2 .8 .0 Physical Composite 92 76 80 83 89 81 89 77 74 83 83 86 97 83 183 196 221 185 81 81 87 181 82 86 118 83 79 82 88 86 78 78 95 78 80 81 95 82 271 259 247 255 84 84 89 87 .7 .2 .1 .7 .4 .8 .4 .9 .6 .7 .8 .6 .2 .4 .3 .9 .8 .9 .7 .7 .0 .7 .3 .6 .1 .0 .2 .2 .9 .2 .7 .8 .7 .6 .0 .7 .3 .3 .8 .5 .3 .2 .4 .4 .1 .3 B-5 September 1995 ------- Comparison ofVOA Compositing Procedures EPISODE 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 4595 4573 4575 4593 Phase II Data - Percent of Spi> Purcfe Device Compositincf ( iy ANALYTE DIETHYL ETHER DIETHYL ETHER DIETHYL ETHER DIETHYL ETHER ETHYL BENZENE ETHYL BENZENE ETHYL BENZENE ETHYL BENZENE M-XYLENE M-XYLENE M-XYLENE M-XYLENE METHYL ETHYL KETONE METHYL ETHYL KETONE METHYL ETHYL KETONE METHYL ETHYL KETONE METHYLENE CHLORIDE METHYLENE CHLORIDE METHYLENE CHLORIDE METHYLENE CHLORIDE O- + P-XYLENE O- + P-XYLENE O- + P-XYLENE O- + P-XYLENE P-DIOXANE P-DIOXANE P-DIOXANE P-DIOXANE TETRACHLOROETHENE TETRACHLOROETHENE TETRACHLOROETHENE TETRACHLOROETHENE TOLUENE TOLUENE TOLUENE TOLUENE TRANS- 1, 3-DICHLOROPROPENE TRANS- 1, 3-DICHLOROPROPENE TRANS- 1, 3-DICHLOROPROPENE TRANS- 1, 3-DICHLOROPROPENE TRICHLOROETHENE TRICHLOROETHENE TRICHLOROETHENE TRICHLOROETHENE TRICHLOROFLUOROMETHANE TRICHLOROFLUOROMETHANE TRICHLOROFLUOROMETHANE :e Recov< iCont' ) lathemat eries ical Composite 94 101 112 91 97 93 56 95 46 32 141 56 79 103 99 102 86 76 93 84 43 40 120 58 87 100 98 126 92 84 95 92 94 91 54 100 96 80 93 84 103 83 93 95 .6 .2 .8 .3 .7 .5 .3 .8 .8 .4 .5 .8 .1 .2 .8 .5 .0 .3 .8 .8 .3 .9 .9 .7 .1 .0 .7 .6 .4 .8 .3 .6 .8 .3 .7 .6 .4 .0 .1 .8 .2 .8 .6 .4 . Physical Composite 82 100 102 71 74 83 48 78 34 33 78 44 74 103 100 103 72 72 88 72 37 40 79 48 83 110 98 117 80 76 83 81 72 82 49 48 81 78 91 102 81 77 83 81 .9 .6 .0 .3 .6 .7 .8 .1 .1 .7 .9 .9 .3 .0 .8 .2 .4 .6 .1 .5 .5 .0 .4 .8 .0 .2 .7 .8 .4 .2 .6 .1 .1 .9 .5 .9 .1 .9 .1 .7 .6 .0 .1 .4 September 1995 B-6 ------- Comparison of VOA Compositing Procedures Phase II Data - Percent of Spike Recoveries EPISODE 4595 4573 4573 4575 4593 4595 Purge Device Compositing (Conf) Mathematical ANALYTE Composite TRICHLOROFLUOROMETHANE VINYL ACETATE VINYL CHLORIDE VINYL CHLORIDE VINYL CHLORIDE VINYL CHLORIDE 240.0 97.1 91.0 104.1 103.3 Physical Composite 217.9 74.9 81.4 92.9 84.3 B-7 September 1995 ------- ------- Appendix C Phase III Data ------- ------- Comparison of VOA Compositing Procedures EPISODE 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 4617 4618 Phase III Data ANALYTE 1,1,1 -TRICHLOROETHANE 1,1,1 -TRICHLOROETHANE 1,1,2,2 -TETRACHLOROETHANE 1,1,2, 2 -TETRACHLOROETHANE 1,1,2 -TRICHLOROETHANE 1,1,2 -TRICHLOROETHANE 1 , 1-DICHLOROETHANE 1 , 1-DICHLOROETHANE 1 , 1-DICHLOROETHENE 1 , 1-DICHLOROETHENE 1 , 2 -DICHLOROETHANE 1 , 2 -DICHLOROETHANE 1 , 2 -DICHLOROPROPANE 1 , 2 -DICHLOROPROPANE 2-CHLOROETHYLVINYL ETHER 2-CHLOROETHYLVINYL ETHER ACRYLONITRILE ACRYLONITRILE BENZENE BENZENE BROMODICHLOROMETHANE BROMODICHLOROMETHANE BROMOFORM BROMOFORM BROMOMETHANE BROMOMETHANE CARBON TETRACHLORIDE CARBON TETRACHLORIDE CHLOROBENZENE CHLOROBENZENE CHLOROETHANE CHLOROETHANE CHLOROFORM CHLOROFORM CHLOROMETHANE CHLOROMETHANE DIBROMOCHLOROMETHANE DIBROMOCHLOROMETHANE DIETHYL ETHER DIETHYL ETHER ETHYL BENZENE ETHYL BENZENE METHYL ETHYL KETONE METHYL ETHYL KETONE METHYLENE CHLORIDE METHYLENE CHLORIDE P-DIOXANE P-DIOXANE TETRACHLOROETHENE TETRACHLOROETHENE TOLUENE TOLUENE TRANS-1, 2-DICHLOROETHENE TRANS- 1, 2-DICHLOROETHENE TRANS-1 , 3 -DICHLOROPROPENE TRANS-1, 3 -DICHLOROPROPENE TRICHLOROETHENE TRICHLOROETHENE VINYL CHLORIDE VINYL CHLORIDE - Percent of Spike Recoveries Mathematical Automated Automated Composite 76. 67. 102. 107 . 86. 88. 79. 73 . 72. 66. 93 . 88. 83 . 78. 90. 101. 100. 110. 101 . 74. 72 . 89. 46. 89. 78. 79. 55. 66. 91. 79. 82. 78. 89. 83 , 84, 80. 55, 85, 106 106 77 70 103 126 104 85 62 102 69 63 75 71 77 71 79 82 72 65 78 75 1 7 4 4 8 4 5 2 r 7 8 2 7 4 9 7 5 7 4 6 6 3 4 , 0 , 8 , 1 2 .2 . 5 . 0 . 5 . 6 2 . 5 .1 . 0 . 3 . 5 .6 . 2 .8 . 0 .6 . 8 .7 . 1 . 6 . 0 .9 .9 .2 . 1 . 3 .4 . 3 .6 .2 . 1 Sampler 78. 72. 115. 111. 93. 91. 80. 77. 79. 70. 95. 94. 81. 82. 117. 121. 109. 114. 107 . 77. 72. 92. 33 . 81. 81. 82. 56. 67. 95. 80. 88. 81. 97. 86. 91. 85. 54. 90. 112, 111. 81, 71 96, 125 101 90 52 108 74 62 77 73 92 75 83 82 69 67 85 79 9 4 9 4 7 1 7 3 4 6 5 1 6 0 3 0 5 1 4 8 7 5 7 5 3 9 0 2 9 1 .6 .9 .8 .1 .1 .3 .6 .7 .2 .2 .1 .3 .9 .1 .3 .4 .0 .0 .9 .8 .8 .8 .2 .0 .6 .1 .4 .1 .1 .0 Compositor 63 . 61. 87. 107 . 82. 90. 73 . 71. 62. 57. 87 . 88. 75. 78. 71. 113 . 96. 113 . 97 . 73 . 59. 85. 26. 97. 69. 77 . 38. 37 . 76. 63 . 75. 74. 91. 80. 80. 78. 36. 84. 106 111 60 55 120 128 93 86 57 101 52 40 54 60 68 68 54 71 55 51 68 69 9 4 7 1 0 5 0 3 4 7 6 7 3 0 8 3 6 2 3 1 .2 7 2 .5 .6 .2 .9 , 1 .1 .9 .7 .3 .4 .2 .1 .2 .4 .9 .8 .6 .4 .6 .3 . 6 .5 .3 .6 .4 .0 .6 .5 .1 .1 .3 .8 .1 .0 .2 .7 .5 Physical Composite 61. 55. 86. 106. 85. 77. 62. 61. 60. 52. 83. 85. 68. 70 . 112. 109. 106. 114 . 82 . 62. 61. 80 . 126. 64. 65. 48. 54. 78. 70. 66. 60. 75. 70. 67. 61. 55, 86. 92, 100, 72 59 92 132 91 73 73 109 69 49 48 58 66 58 71 65 61 51 60 57 4 5 4 8 8 3 8 9 6 5 2 0 5 9 6 6 5 8 8 5 2 2 8 2 .9 .2 .6 ,2 .3 ,3 .3 .4 .2 .3 .9 .3 . 6 .6 .0 .1 .7 .7 .0 .4 .8 .4 .9 .4 .7 .5 .9 .6 .4 .8 .1 .5 .7 .2 .9 C-l September 1995 ------- ------- |