wEPA
United States Environmental Office of Water EPA-822-R-02-020
Protection Agency Washington, DC 20460 March 2002
METHODS FOR EVALUATING WETLAND CONDITION
#10 Using Vegetation To Assess
Environmental Conditions in Wetlands
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wEPA
United States Environmental Office of Water EPA-822-R-02-020
Protection Agency Washington, DC 20460 March 2002
METHODS FOR EVALUATING WETLAND CONDITION
#10 Using Vegetation To Assess
Environmental Conditions in Wetlands
Major Contributors
Department of Biology, Kenyon College
Siobhan Fennessy
Minnesota Pollution Control Agency
Mark Gernes
Ohio Environmental Protection Agency
John Mack
Penn State Cooperative Wetlands Center
Denice Heller Wardrop
Prepared jointly by:
The U.S. Environmental Protection Agency
Health and Ecological Criteria Division (Office of Science and Technology)
and
Wetlands Division (Office of Wetlands, Oceans, and Watersheds)
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NOTICE
The material in this document has been subjected to U. S. Environmental Protection Agency (EPA)
technical review and has been approved for publication as an EPA document. The information
contained herein is offered to the reader as a review of the "state of the science" concerning wetland
bioassessment and nutrient enrichment and is not intended to be prescriptive guidance or firm advice.
Mention of trade names, products or services does not convey, and should not be interpreted as
conveying official EPA approval, endorsement, or recommendation.
APPROPRIATE CITATION
U.S. EPA. 2002. Methods for Evaluating Wetland Condition: Using Vegetation To Assess
Environmental Conditions in Wetlands. Office of Water, U.S. Environmental Protection Agency,
Washington, DC. EPA-822-R-02-020.
This entire document can be downloaded from the following U.S. EPA websites:
http://www.epa.gov/ost/standards
http://www.epa.gov/owow/wetlands/bawwg
11
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CONTENTS
FOREWORD vi
LIST OF "METHODS FOR EVALUATING WETLAND
CONDITION" MODULES vii
SUMMARY 1
PURPOSE 1
BACKGROUND 1
PLANTS AS INDICATORS 2
ADVANTAGES OF USING VEGETATION IN BIOLOGICAL ASSESSMENT 2
CONSIDERATIONS FORSAMPLING DESIGN 3
FIELD METHODS 5
DATA ANALYSIS 12
LIMITATIONS OF CURRENT KNOWLEDGE-RESEARCH NEEDS 17
REFERENCES 19
APPENDIX A 23
APPENDIX B 27
APPENDIX C 32
APPENDIX D 36
LIST OF TABLES
TABLE l: SOME POTENTIAL METRICS FOR WETLAND PLANTS
INCLUDING HOW THEY COULD BE SCORED, HOW THEY
WOULD RESPOND TO HUMAN DISTURBANCE, AND
GEOGRAPHIC AREAS OF THE U.S. WHERE THEY HAVE
BEEN TESTED 16
iii
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TABLE C-l: SCORING CRITERIA FOR VEGETATION-BASED METRICS
IN THE MINNESOTA IVI 33
LIST OF FIGURES
FIGURE l: THREE-TIERED FLOW DIAGRAM USED TO RANK WETLANDS
ALONG A GRADIENT OF HUMAN DISTURBANCE 5
FIGURE 2: EXAMPLE OF HOW TRANSECTS MIGHT BE ESTABLISHED
IN A WETLAND 8
FIGURE 3: EXAMPLE OF POINT-QUARTER SAMPLING AT TWO
RANDOMLY SELECTED FOREST LOCATIONS 1O
FIGURE 4: RELATIONSHIP BETWEEN FQAI SCORES AND BIOMASS
PRODUCTION IN EIGHT CENTRAL OHIO
EMERGENT WETLANDS 15
FIGURE A-l: PROPORTION OF PLANT SPECIES THAT WERE MISSING
DOMINANCE OR DENSITY DATA AT THREE WETLANDS
RESAMPLED IN 1 999 USING RELEVE METHOD 24
FIGURE A-2: NUMBER OF SPECIES FOUND AT THREE WETLANDS
SAMPLED USING TRANSECT-QUADRAT, TRANSECT-BELT,
AND RELEVE METHODS 25
FIGURE A-3: FLORISTIC QUALITY ASSESSMENT INDEX (FQAI) SCORE
AT THREE WETLANDS SAMPLED USING TRANSECT-QUADRAT,
TRANSECT-BELT, AND RELEVE METHODS 25
FIGURE A-4: INTERIM VEGETATION INDICES OF BIOLOGICAL
INTEGRITY OBI) SCORES FOR 45 WETLANDS IN THE
EASTERN CORNBELT PLAINS ECOREGION OF OHIO 26
FIGURE B-l: VEGETATION INDEX OF BIOLOGICAL INTEGRITY SCORE BY
HYDROGEOMORPHIC CLASSIFICATION FOR N = 65
FORESTED AND EMERGENT WETLANDS IN THE STATE OF
OHIO 28
FIGURE B-2: Box AND WHISKER PLOTS OF EMERGENT AND FORESTED
REFERENCE (LEAST-IMPACTED) WETLANDS 29
IV
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FIGURE B-3: RELATIVE COVER OF TOLERANT HERB AND SHRUB
STRATUM SPECIES FOR REFERENCE (LEAST IMPACTED)
AND NONREFERENCE (ALL OTHER SITES) OF
FORESTED AND EMERGENT WETLANDS (N = 65) FOR
FOUR ECOLOGICAL REGIONS IN THE STATE OF OHIO 29
FIGURE B-4: FQAI SCORES FOR 45 WETLANDS IN THE EASTERN
CORN BELT PLAINS ECOREGION OF OHIO 3O
FIGURE B-5: FLORISTIC QUALITY ASSESSMENT INDEX (FQAI) SCORE BY
HYDROGEOMORPHIC CLASSIFICATION FOR N = 65
FORESTED AND EMERGENT WETLANDS IN OHIO 3O
FIGURE D-l: SEDIMENT TOLERANCE GROUPS OF WETLAND PLANTS
IN SLOPE WETLANDS OF CENTRAL PENNSYLVANIA
WETLANDS 38
v
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FOREWORD
In 1999, the U. S. Environmental Protection Agency (EPA) began work on this series of reports entitled
Methods for Evaluating Wetland Condition. The purpose of these reports is to help States and
Tribes develop methods to evaluate (1) the overall ecological condition of wetlands using biological
assessments and (2) nutrient enrichment of wetlands, which is one of the primary stressors damaging
wetlands in many parts of the country. This information is intended to serve as a starting point for States
and Tribes to eventually establish biological and nutrient water quality criteria specifically refined for
wetland waterbodies.
This purpose was to be accomplished by providing a series of "state of the science" modules concerning
wetland bioassessment as well as the nutrient enrichment of wetlands. The individual module format
was used instead of one large publication to facilitate the addition of other reports as wetland science
progresses and wetlands are further incorporated into water quality programs. Also, this modular
approach allows EPA to revise reports without having to reprint them all. A list of the inaugural set of
20 modules can be found at the end of this section.
This series of reports is the product of a collaborative effort between EPAs Health and Ecological
Criteria Division of the Office of Science and Technology (OST) and the Wetlands Division of the
Office of Wetlands, Oceans and Watersheds (OWOW). The reports were initiated with the support
and oversight of Thomas J. Danielson (OWOW), Amanda K. Parker and Susan K. Jackson (OST),
and seen to completion by Douglas G. Hoskins (OWOW) and Ifeyinwa F. Davis (OST). EPArelied
heavily on the input, recommendations, and energy of three panels of experts, which unfortunately have
too many members to list individually:
• Biological Assessment of Wetlands Workgroup
• New England Biological Assessment of Wetlands Workgroup
• Wetlands Nutrient Criteria Workgroup
More information about biological and nutrient criteria is available at the following EPA website:
http ://www. epa. gov/ost/standards
More information about wetland biological assessments is available at the following EPA website:
htto ://www.epa. gov/owow/wetlands/bawwg
VI
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LIST OF "METHODS FOR EVALUATING WETLAND
CONDITION" MODULES
MODULE # MODULE TITLE
1 INTRODUCTION TO WETLAND BIOLOGICAL ASSESSMENT
2 INTRODUCTION TO WETLAND NUTRIENT ASSESSMENT
3 THE STATE OF WETLAND SCIENCE
4 STUDY DESIGN FOR MONITORING WETLANDS
5 ADMINISTRATIVE FRAMEWORK FOR THE IMPLEMENTATION OF A
WETLAND BIOASSESSMENT PROGRAM
6 DEVELOPING METRICS AND INDEXES OF BIOLOGICAL INTEGRITY
7 WETLANDS CLASSIFICATION
8 VOLUNTEERS AND WETLAND BIOMONITORING
9 DEVELOPING AN INVERTEBRATE INDEX OF BIOLOGICAL
INTEGRITY FOR WETLANDS
10 USING VEGETATION TO ASSESS ENVIRONMENTAL CONDITIONS
IN WETLANDS
11 USING ALGAE TO ASSESS ENVIRONMENTAL CONDITIONS IN
WETLANDS
12 USING AMPHIBIANS IN BlOASSESSMENTS OF WETLANDS
13 BIOLOGICAL ASSESSMENT METHODS FOR BIRDS
14 WETLAND BIOASSESSMENT CASE STUDIES
15 BIOASSESSMENT METHODS FOR FISH
16 VEGETATION-BASED INDICATORS OF WETLAND NUTRIENT
ENRICHMENT
17 LAND-USE CHARACTERIZATION FOR NUTRIENT AND SEDIMENT
RISK ASSESSMENT
18 BlOGEOCHEMICAL INDICATORS
19 NUTRIENT LOAD ESTIMATION
2O SUSTAINABLE NUTRIENT LOADING
vn
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SUMMARY
Tyegetation has been shown to be a sensitive
V measure of anthropogenic impacts to wetland
ecosystems. As such it can serve as a means to
evaluate best management practices, assess resto-
ration and mitigation projects, prioritize wetland-
related resource management decisions, and estab-
lish aquatic life use standards for wetlands. The
basic steps necessary for developing a vegetation-
based wetland biological assessment and monitor-
ing program are relatively straightforward, but their
simplicity belies their effectiveness. By building upon
such monitoring tools, we will be able to more fully
incorporate wetlands into water quality assessment
programs.
PURPOSE
rhe purpose of this module is to introduce the
scientific basis for using wetland vegetation to
assess the biological integrity of wetlands, review
methods for sampling vegetation communities, dis-
cuss the techniques by which biological metrics are
developed, and present examples of metrics and
indices that have been used successfully. We dis-
cuss here how the composition of the plant com-
munity and the predictable changes that result from
human activities can act as sensitive indicators of
the biological integrity of wetland ecosystems. This
information has many potential applications includ-
ing conducting an inventory, monitoring the status
and trends of wetland ecosystems, performing an
impact assessment, and setting goals for and/or
monitoring mitigation and restoration projects.
BACKGROUND
T/'egetation is perhaps the most conspicuous
V feature of wetland ecosystems and has been
used extensively as an indicator of the presence of
wetlands themselves, their boundaries, and as abasis
for many wetland classification schemes. Wetland
plants are commonly defined as those "growing in
water or on a substrate that is at least periodically
deficient in oxygen as a result of excessive water
content" (Cowardinetal. 1979). This term includes
both herbaceous (vascular and nonvascular) and
woody species. Wetland plants may be floating,
floating-leaved, submerged, or emergent and may
complete their life cycle in still or flowing water, or
on inundated or noninundated hydric soils (Cronk
and Fennessy 2001).
One key to understanding why plants are consid-
ered "one of the best indicators of the factors that
shape wetlands within their landscape" (Bedford
1996), is to understand the contributions they make
to wetland ecosystems more generally. These con-
tributions include (Wiegleb 1988, Mitsch and
Gosselink2000):
• Wetland vegetation is at the base of the food
chain and, as such, is a primary pathway for
energy flow in the system. Through the photo-
synthetic process, plants link the inorganic en-
vironment with the biotic one. Primary pro-
duction (or plant biomass production) in wet-
lands varies, but some herbaceous wetlands
have extremely high levels of productivity, ri-
valing those of tropical rain forests.
• Wetland vegetation provides critical habitat
structure for other taxonomic groups, such as
epiphytic bacteria, phytoplankton, and some
species of algae, periphyton, macroinverte-
brates, amphibians, and fish. The composition
and diversity of the plant community influences
diversity in these other taxonomic groups.
• Strong links exist between vegetation and wet-
land water chemistry. Plants remove nutrients
through uptake and accumulation in tissues, but
they also act as a nutrient pump by moving com-
pounds from the sediment and into the water
column. The ability of vegetation to improve
water quality through the uptake of nutrients,
metals, and other contaminants is well docu-
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mented (e.g., Gersberg et al. 1986, Reddy et
al. 1989,Peverlyetal. 1995,Raietal. 1995,
Tanner etal. 1995).
• Vegetation influences the hydrology and sedi-
ment regime through processes such as sedi-
ment and shoreline stabilization, or by modify-
ing currents and helping to desynchronize flood
peaks.
PLANTS AS INDICATORS
T)lants are excellent indicators of wetland condi-
JT tion for many reasons including their relatively
high levels of species richness, rapid growth rates,
and direct response to environmental change. Many
human-related alterations to the environment that
act to degrade wetland ecosystems cause shifts in
plant community composition that can be quanti-
fied easily. Individual species show differential tol-
erance to a wide array of stressors. Thus as envi-
ronmental conditions vary, community composition
shifts in response. Plant communities have been
shown to change in response to hydrologic alter-
ations (e.g., Gosselink and Turner 1978, van der
Valk 1981, Spence 1982, Squires and van der Valk
1992, Wilcox 1995), nutrient enrichment (e.g., Pip
1984, Kadlec and Bevis 1990, Templer et al. 1998,
Craft and Richardson 1998), sediment loading and
turbidity (e.g., van der Valk 1981,1986, Sager et
al. 1998, Wardrop and Brooks 1998), and metals
and other pollutants. These patterns can be inter-
preted and used to diagnose wetland impacts. Be-
cause they represent a diverse assemblage of spe-
cies with different adaptations, ecological tolerances,
and life history strategies, the composition of the
plant community can reflect (often with great sensi-
tivity) the biological integrity of the wetland.
A wetland's hydrologic regime can be thought of
as a master variable with respect to the structure
(e.g., species richness) and function (e.g., primary
production) of the plant community, and much re-
search has been done on the links between hydrol-
ogy and plant community dynamics (Mitsch and
Gosselink 2000). Factors related to the hydro-
logic regime that affect wetland plants include wa-
ter depth (Spence 1982, Grace and Wetzel 1982,
1998), water chemistry (Ewel 1984, Pip 1984, Rey
Benayas et al. 1990, Rey Benayas and Scheiner
1993) and flow rates (Westlake 1967, Lugo et al.
1988, Nilsson 1987, Carr et al. 1997). As hydrol-
ogy changes, other environmental conditions change
as well. Thus, hydrology influences community com-
position and productivity by influencing the avail-
ability of nutrients (Neill 1990), soil characteristics
(Barko and Smart 1978, 1983), and the deposi-
tion of sediments (Barko and Smart 1979).
Water quality also has a strong bearing on com-
munity structure (Grootjans et al. 1998, Rey
Benayas et al. 1990, Rey Benayas and Scheiner
1993). Fens, for example, tend to be high in cal-
cium and magnesium bicarbonates with circum-
neutral pH (Wilcox et al. 1986). Bogs, on the
other hand, depend solely on precipitation for their
water supply and tend to be nutrient poor and of
low pH. Plants adapted to bog conditions often
exhibit a number of mechanisms that serve to
conserve or help acquire nutrients, including
evergreen leaves, carnivory, or nitrogen fixation.
ADVANTAGES OF USING
VEGETATION IN
BIOLOGICAL
ASSESSMENT
TT/etland plants, both vascular and nonvascular,
V V are commonplace, and they exist in sufficient
richness to provide clear and robust signals of hu-
man disturbance. They have been used effectively
to distinguish environmental stressors including hy-
drologic alterations, excessive siltation, nutrient en-
richment, and other types ofhuman disturbance (van
der Valk 1981, Moore and Keddy 1989,
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Galatowitsch 1993, Wilcox 1995, Kantrud and
Newton 1996, Stromberg et al. 1996, Philippi et
al. 1998). Vegetation is useful to evaluate wetland
integrity because:
• Plants are found in all wetlands.
• Plants are primarily immobile (save for a few
free-floating species). Because they reflect
the temporal, spatial, chemical, physical, and
biological dynamics of a system, they can
indicate any long-term, chronic stress it under-
goes.
• Plant taxonomy is well known, and excellent
field guides are available for all regions.
Experienced field biologists can identify genus or
species relatively easily because:
• A great diversity of species exists with differing
responses to human disturbance.
• Ecological tolerances are known for many spe-
cies, and thus changes in community composi-
tion might be used to diagnose the stressor re-
sponsible. For example, plant responses to
changing hydrology are reasonably predictable
(see above).
• Sampling techniques are well developed and
extensively documented.
• Similar sampling techniques can be used in both
freshwater and saltwater systems.
• Functionally or structurally based vegetation
guilds have been proposed for some regions.
Despite the many advantages to developing bio-
logical assessment techniques based on vegetation,
limitations should be recognized:
• A lag may occur in the response time to
stressors, particularly in long-lived species.
When this is the case the species present may
not be indicative of the stressors present and/
or the overall biological integrity.
• Plant identification to species level can be
laborious, or restricted to narrow periods
during the field season. Several assemblages,
such as the grasses and sedges, may be
particularly difficult to identify to species. Con-
cern is sometimes expressed about the skill
that good field botany requires. However, with
a modest amount of training, most species
in a given class of wetland can be easily
learned, or the art of keying out species can be
learned.
• Sampling techniques for some assemblages,
such as the submerged species, can be diffi-
cult; thus it is possible to miss or erroneously
sample a group of species that could provide
strong signals on the condition of a site.
• Vegetation sampling is generally limited to the
growing season.
• Research or literature on plant species
responses to specific stressors is not well
developed. Adamus and Gonyaw (2000) esti-
mate that only 17% of all wetland plant species
have been the subject of studies that detail their
response to specific stressors, although the
general tolerance of many species to human
change to the environment is more well
known.
CONSIDERATIONS FOR
SAMPLING DESIGN
A sound framework for developing biological in-
^~L dicators based on wetland vegetation should
include several key components.
OBJECTIVES OF THE PROJECT
The vegetation community changes seasonally,
and these patterns can differ among wetland types.
Standardized field methods must be tested and re-
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fined to ensure that a consistent sampling effort is
made at each site. Consideration must be given to
the type of data that will be collected (species in-
ventory, cover, stem counts, etc.), the sampling win-
dow (i.e., the seasonal period) that will be used to
characterize the vegetation, the sampling technique
that will be employed, the number of samples that
will be collected, and retention of voucher speci-
mens. The same considerations apply to any meth-
ods that will be performed subsequent to field sur-
veys either in the laboratory or the office. Projects
may be designed to develop or test metrics that are
sensitive to specific stressors such as hydrologic
alteration or nutrient enrichment. In this case wet-
lands that vary in their degree of impact should be
included. This approach allows for hypothesis test-
ing, i.e., whether the conditions at a site differ sig-
nificantly from those found in a population of refer-
ence wetlands (see Module 11 for further examples).
WETLAND CLASSIFICATION
Because the composition of wetland plant com-
munities varies by wetland type (class), it is neces-
sary to group "like-kind" wetlands into classes that
are structurally and functionally similar. In this way
natural variability is reduced, making detection of
human-induced variability easier. This leads to more
meaningful comparisons between wetlands and cre-
ates a more sensitive tool for decisionmakers. There
are several well-established wetland classification
schemes, including the hydrogeomorphic (HGM)
approach (Brinson 1993), which has proven to be
useful in classifying wetlands for biological assess-
ment (e.g., Fennessy et al. 1998b), as has the clas-
sification system employed in the National Wetland
Inventory (Cowardinetal. 1979). Ecoregions have
also been used to classify wetlands for vegetation
analysis (Omernik 1987). (See Module 7 for fur-
ther discussion on wetland classification.)
DEFINE THE REFERENCE CONDITION
A crucial component of a biological assessment
program is the careful selection of least-impacted
reference sites. Reference sites are wetlands of the
same class that define the best possible condition
for that class. Reference sites serve as the standard
against which other sites are judged (Yoder and
Rankin 1993, Karr and Chu 1999).
SELECT WETLANDS THAT REPRESENT
THE FULL RANGE OF HUMAN
DISTURBANCE
In addition to reference sites, other sites should
be selected to represent the full range of human dis-
turbance for each wetland class. This makes it pos-
sible to evaluate the response of the wetland plant
community to increasing "doses" of human activity
(i.e., construct a dose-response curve) (Karr and
Chu 1999). There is currently no standard method
to quantify human disturbance at a site, so many
projects have relied on surrogate measures such as
percent impervious surface (Richter and Azous
1994) or percent agricultural land use in the water-
shed. If assessing plant community response to nu-
trient enrichment is the objective, potential metrics
can be tested against individual environmental mea-
sures such as soil phosphorus content or turbidity
of the water column. Another approach is devel-
opment of a qualitative index of human disturbance
based on dominant land use surrounding the wet-
land, buffer characteristics, and the degree of hy-
drologic alteration to the site (Figure 1) (Fennessy
etal. 1998a, Lopez and Fennessy in press). The
criteria for judging whether a site is least or most
impacted are, in part, subjective, but there are sev-
eral standardized, semiquantitative checklists for
evaluating human disturbance at different wetlands.
These include the Ohio Rapid Assessment Method
(Ohio EPA 2000) and a "Stressor Checklist" de-
veloped for wetlands in Pennsylvania (Wardrop and
Brooks, personal communication). Both techniques
provide a means to standardize the evaluation of
human impact.
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ity. Data requirements, level of sampling effort, and
types of ancillary data collected all hinge on the goals
of the study and the anticipated uses of the results
(biocriteria development, setting goals for mitiga-
tion wetlands, monitoring wetland condition, etc).
In addition, the development of any biological as-
sessment tools will likely be the effort of a team of
people with various areas of expertise and experi-
ence. We recommend that interdisciplinary crews
conduct biological assessment during both the plan-
ning and data collection phases if at all possible.
ORGANIZING DATA COLLECTION
There are several essential steps in developing
vegetation-based bioassessment protocols. These
include (1) decisions about the frequency and in-
tensity of sampling, (2) site reconnaissance, and (3)
decisions about the sampling technique(s) that will
be used in the field.
TIMING AND INTENSITY OF SAMPLING
Sampling season
The establishment of a standard sampling window
will help ensure that representative results are ob-
tained at each site and that valid comparisons can
be made between different wetlands. Wetland
plants pose a challenge in terms of their identifica-
tion and because different species reach maturity
or flower at different times during the growing sea-
son. Thus, some species may be present but can-
not be identified because of a lack of flowing parts
(a critical feature used in identification). Members
of Asteraceae(e.g.,^5tersp. [asters]), which tend
to bloom late in the growing season, are a classic
example. Temporal variability in the plant commu-
nity is a fiinction of geographic location and the class
of wetland ecosystem being studied. Any decision
will involve trade-offs because no one sampling
period will be able to capture all species. Setting
an index period that corresponds to the peak ma-
turity of the community as a whole is generally con-
sidered most appropriate, particularly if the goal is
to assess a wetland in a single visit. Interestingly,
three States that have established index periods have
arrived at nearly the same period. These include
Minnesota (June 15-August 15), Ohio (June 15-
August 30), and Pennsylvania (June 15-August 15).
Forested wetlands appear to be less sensitive in
terms of when the vegetation is surveyed. For ex-
ample, in the southeastern States, the changes that
occur in the plant community over the growing
season are relatively minor (see Module 16: Veg-
etation-Based Indicators of Wetland Nutrient
Enrichment). In this case, it may be desirable to
coordinate vegetation sampling periods with the sam-
pling period for other assemblages.
Number of survey plots
In any study, the number of plots to sample is an
important consideration. The appropriate number
can be determined by plotting species numbers (or
the cover of a given species) as a function of the
number of quadrats sampled and then identifying
where species richness "levels off." Daubenmire
(1959) systematically investigated how sampling ef-
fort affects the number of species recorded at a site
and found apoint of diminishing returns was reached
when 40 quadrats had been sampled. In the initial
10 quadrats some species were underrepresented
whereas others were overrepresented. At the point
where 30 to 40 quadrats had been sampled, the
data had leveled off, and increasing the sample size
to 50 quadrats did not give any additional informa-
tion. Another recommendation is that a total of 1 %
of the total wetland area be sampled (Krebs 1999).
One method to accomplish this, which several States
have adopted, is use of releve techniques. Releve
plots can adequately represent the vegetation of a
site with a single large plot (e.g., 100m2). Regard-
less of the method selected, tradeoffs regarding sam-
pling effort must be made in light of the time and
resources available.
6
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SITE RECONNAISSANCE
Before in-depth field sampling begins, general in-
formation should be collected on the landscape set-
ting of the site, its hydrologic features, and other
general characteristics. Many valuable observa-
tions can be made from the edges of the wetland
that will describe obvious stressors such as hydro-
logic alterations (berms, culverts), or the extent of
vegetation cover and the characteristics of any buffer
areas. Buffer areas are typically defined as the
100 m of land surrounding the wetland boundary
(Magee et al. 1993). Land use and/or the domi-
nant vegetation communities within the buffer should
be recorded. Depending on the goals of the project
it may be desirable to collect samples to quantify
characteristics of the buffer.
Inspecting the topography and landscape features
that surround the site can provide much information
on the context of a site. There are also many char-
acteristics that can be recorded about the wetland
itself, including its shape and approximate size, the
general distribution of wetland vegetation and open
water, interspersion of plant communities, the type
of buffer, hydrological features including surface
water inflows or outflows, and human-made water
control structures.
CHOICE OF VEGETATION FIELD
SAMPLING METHODS
Below are examples of vegetation sampling pro-
tocols that can integrate the above considerations
of plot locations, shape, size, and number, and that
have been used successfully in wetland
bioassessment projects.
Standard releve (Braun-Blanquet) for
emergent vegetation
The releve approach has been adapted from
Mueller-Dombois and Ellenberg (1974). Prior to
sampling, each wetland is evaluated briefly for over-
all community structure. A 100 m2 plot is estab-
lished in atypical or "representative" location within
the emergent plant community. Plants in the plot are
inventoried and the cover class (abundance) of each
plant taxon is estimated using cover classes
(Almendinger 1987). Similar sampling effort can
also be made in the floating or submerged commu-
nity zones when they are present and wading is
possible.
This sampling technique is easy to apply and prac-
tical, taking a field-trained botanist with an assistant
typically 2 hours or less to complete per site. An
advantage (or disadvantage, depending on one's
view) is fact that sampling is restricted to the domi-
nant vegetation community represented at the site.
One negative side is that it is not very data intensive
for spatially heterogeneous or complex communi-
ties. In some cases it may be desirable to collect
samples across all communities present. This can
be done using multiple releve plots, although this is
more difficult and time-consuming.
The required equipment includes two 50-m mea-
suring tapes, four corner markers or posts (8-foot
rebar works well), a clipboard with data forms, and
plant collecting materials. Data gathered in the releve
plot can be augmented with a more complete list of
taxa occurring at the entire site, for example, as
collected by a walk-through (care should be taken
in this case as sampling effort can vary depending
on the investigator). A modification of the releve
method has been adopted in Ohio (see
Appendix A).
Transect sampling
There are numerous variations on the use of
transects for sampling vegetation in herbaceous,
shrub-scrub, or wooded wetlands. The location of
transects within a wetland can be determined in sev-
eral ways. One approach is to locate them either
randomly (using a random numbers table for ex-
ample) or systematically (e.g., located at fixed in-
7
-------
tervals) perpendicular to abaseline. Baselines are
generally established just outside the wetland par-
allel to its longest axis (Figure 2). Transect loca-
tions can also be determined using a stratified ran-
dom design in which different portions of the site
are targeted for sampling to ensure that the habitat
complexity of the wetland is represented, but within
those zones transects are located randomly.
Transects may be a single line, or a belted transect
can be used in which data are recorded in a zone
extending on either side of the line (often to a dis-
tance of 1 m). Often a transect line is used in com-
bination with quadrats placed at random or regular
intervals along the line.
Data can be collected along the transects using
several techniques:
• All vegetation in each quadrat is sampled (see
section on quadrats below).
• The line-intercept method is frequently used.
In this technique, the transect line is thought of
as a vertical plane that is perpendicular to the
ground. All plant canopies projecting through
the plane (over the line) are counted. The total
decimal fraction of the line covered by each
species and multiplied by 100 is equal to its
percent cover (Barbour et al. 1987). Total
cover can be more than 100% using this
technique.
Quadrat methods
Many possible sampling schemes are available for
sampling using quadrats, and there are variations
appropriate for all vegetation types. Often, com-
munities or stands are selected subjectively, but then
sampled using randomly located quadrats (strati-
fied random technique). Common variations include
(Barbour etal. 1987):
Transect end-point -*.
Upland
120m 108m
Transect start point
FIGURE 2: EXAMPLE OF HOW TRANSECTS MIGHT BE ESTABLISHED IN A WETLAND.
IN THIS CASE A BASELINE IS ESTABLISHED ALONG THE LONG AXIS OF THE SITE,
AND TRANSECTS CAN BE RUN BOTH PARALLEL AND PERPENDICULAR TO THIS AXIS.
8
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• Locating quadrats in a completely random
fashion
• Locating quadrats in a restricted or stratified
random manner (stratified random sampling).
This is often based on the plant community types
that are present. Placing quadrats randomly
within the various community types helps en-
sure that habitat heterogeneity will be adequately
represented.
Quadrat size, shape, and number: Many meth-
odological studies have been conducted to deter-
mine the precision and accuracy of different quad-
rat types (Krebs 1999). For surveying herbaceous
plant communities, there are two primary issues re-
garding shape: edge effects and habitat heteroge-
neity. Many studies have found that long, narrow
rectangles work best because this shape tends to
cross more patches (i.e., cover more habitat het-
erogeneity) and therefore tends to pick up more
species. Square and round quadrats have been
reported to be less accurate because less hetero-
geneity is encompassed. However, sampling accu-
racy tends to decrease as quadrat shape lengthens
because of edge effects. The longer the perimeter
of a quadrat, the greater the necessity for any field
personnel to make subjective decisions about
whether a plant near the edge is "in" or not. This
kind of decisionmaking leads to possible counting
errors, and results can vary greatly between indi-
viduals. In light of this concern, round quadrats are
considered by many to be the most accurate be-
cause this shape has the smallest perimeter: area ra-
tio (Krebs 1999).
The appropriate quadrat size also varies with the
goals of data collection. If the goal is to collect
cover data, then quadrats may be small. If data on
plant numbers are required, then the decision about
quadrat size is a critical one. Although there are no
fixed rules, several recommendations can be de-
rived from the literature (analysis from Krebs 1999):
• Use a quadrat that is at least twice as large as
the average canopy spread of the largest spe-
cies (Greig-Smith 1964).
• Use a quadrat size that will include only one or
two species (Daubenmire 1968).
• Use a size that allows the most common
species to occur in more than 80% of all
quadrat.
SAMPLING CONSIDERATIONS SPECIFIC
TO FORESTED WETLANDS
For herbaceous plants, quadrats of 1 m2 or 10 m
x 10 m have most commonly been used. For
woody plants, plots of at least 10 m x 10 m are
used. The forestry literature recommends 400-
1,000 m2 as a minimum area to adequately charac-
terize eastern forest communities (Peet et al. 1998).
A number of sampling techniques work well for frees
and shrubs in forested systems, many of which are
plotless methods. These are generally called dis-
tance methods because they are based on distances
between:
• Random individuals (trees or shrubs) and their
nearest neighbors
• Random points and the nearest organism(s)
(Krebs 1999)
Point-quarter method
The point-quarter method is a distance technique
commonly used in forestry studies. A series of ran-
dom points is selected, generally along a transect
line, with the limitation that points should be far
enough apart such that the same individual is not
measured twice at two successive points. The area
around each random point is divided into four 90°
quadrants, and the distance to the nearest tree is
measured in each (Figure 3). In this way four point-
to-free distances are measured at each random point
(Krebs 1999). These data can be used to calcu-
9
-------
late an unbiased estimate of population density as
follows:
where Np=the estimate of population density, n =
number of random points, pi = 3.14159, and(r2tj)
= distance from random point i to the nearest or-
ganism in quadrantj (j = 1,2,3,4; i = l,...,n).
The variance of the population density can be cal-
culated as follows:
Variance (Np) = Np 2/(4n - 2)
Standard error is expressed as:
Standard error of N = [(variance of N )/4n]1/2
Each tree is identified by species, and diameter at
breast height (DBH) is measured. This method is
easy and efficient when site conditions allow one to
quickly divide the area around the random points
into four equal quadrants. The primary criticism of
this method is that because only four trees are mea-
sured at each point, the number of individuals
sampled is often too low to truly represent large
populations.
Bitterlich variable plot method
This is a forestry technique that can be used to
calculate basal area only. The advantage of this
method is that it is extremely fast and has been used
widely in forestry studies. Sampling is done using a
sighting device (either a stick with a crosspiece at
one end, or a prism). The stick is held horizontally
with the crosspiece at the far end and the viewer
slowly turns in a complete circle. Each tree whose
trunk is seen in the line of sight is tallied and identi-
fied by species if its trunk size exceeds the width of
the crosspiece; all other tress are ignored. Total
basal area (m2 Ha'1) for each species is then calcu-
lated as the number of trees of that species divided
by 2. Plot size is variable in this method because
the plot radius is not fixed.
Random
point
Random
point
FIGURE 3: EXAMPLE OF POINT-QUARTER SAMPLING AT TWO RANDOMLY SELECTED
FOREST LOCATIONS. IN EACH CASE, THE SHORTEST POINT-TO-TREE DISTANCE IS
MEASURED IN EACH OF THE FOUR QUADRANTS.
1O
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COLLECTION OF ANCILLARY
DATA TO CHARACTERIZE
ENVIRONMENTAL CONDITIONS
Some physical and chemical data on the site should
be collected in order to interpret and understand
vegetation data. The need for information on the
hydrology and soils of the system, for example, is a
primary reason why many wetland assessment teams
are made up of individuals from several different
disciplines. Some States are developing methods
for assessing wetland functions on the basis of wet-
land HGM (Smith etal. 1995). Data on composi-
tion of wetland plant communities can be used to
assist HGM determinations of wetland function, and
conversely, HGM data can be invaluable in
bioassessment to organize sampling and interpret
the influence of hydrogromorphic factors on wet-
land plant communities. Some examples of hydro-
logical indicators that might be included are:
• Presence of any surface water inflows and
outflows
• Other indicators of wetland hydrology such as
water marks, stained leaves, sediment depos-
its, or soil saturation in top 30 cm (see wetland
delineation manual, ACOE 1987)
• Collection of water samples for analysis. If your
system is seasonally flooded and water chem-
istry data are required, this must be taken into
account when developing the sampling design.
It may be necessary to sample water chemistry
at different times than the vegetation. Param-
eters to analyze might include pH, dissolved
oxygen, conductivity, total phosphorus, soluble
reactive phosphorus, nitrogen (nitrate, ammo-
nia, and total Kjehldahl N), total suspended
solids, turbidity, metals (in some instances), and
total organic carbon. Percent salinity is an ad-
ditional variable to measure in saline wetlands.
Soils can be easily characterized using a standard
soil probe. Some examples of soil data that might
be gathered are:
• Thickness of the organic horizon
• Soil texture
• Organic matter content
• Munsell soil color
• Presence of mottles, their size and color, and
presence of oxidized root channels
Depending on the study goals, it may also be nec-
essary to collect soil samples for laboratory analy-
sis. Standard analysis generally include pH, per-
cent organic matter, and nutrients or perhaps met-
als. Total phosphorus is often a good indicator of
human disturbance and/or the deposition of heavy
sediment loads.
VOUCHERS AND QA/QC
The rigor of QA/QC requirements should be ap-
propriate to the goals of the study and the intended
use of the results. Requirements that are appropri-
ate for a "pilot" study whose purpose is to identify
promising metrics may not be as rigorous as those
of a full-blown regional study whose purpose is to
provide statistical estimates with known confidence
limits regarding the numbers (or proportion) of wet-
lands that are degraded. Depending on project goals
and budget, consider including the following:
• Collect all species that are unknown and return
them to the lab for identification. We recom-
mend pressing plants in the field to ensure the
quality of the samples and allow their preserva-
tion as voucher specimens (e.g., in herbaria) if
that becomes desirable.
• Because of their difficult taxonomy, we recom-
mend collecting all but the most common sedges
(e.g., Carexsp.), grasses, and rushes.
• Confirm unknown species with local university
herbaria or other botanical experts.
• Generally, err on the side of conservatism;
overcollect rather than undercollect.
1 1
-------
• One approach developed in Minnesota is to
establish a column on the field data sheet to
indicate the level of certainty on the identifica-
tion of a given species. This information can
help in later data interpretation.
• If project personnel are changing, then more
confirmation and vouchering will be neces-
sary.
• Calibrate the personnel in terms of judging per-
cent cover and species identification.
• Collect a specimen of each species if possible.
These could then be donated to a local her-
barium. This is especially important during the
reference phase when metrics are being devel-
oped and validated.
• Collect species whose identity is uncertain in
order to confirm the species using standard
floras. In addition, have another person con-
firm the species. That way there is a more de-
fensible record.
• Use rare-plant protocols when necessary (e.g.,
if there are fewer than 20 individuals of a given
species, photograph them only).
One example of a quality assurance field protocol
comes from Ohio EPA(Fennessy et al. 1998). In
a study designed to test a vegetation-based indica-
tor in forested riparian wetlands, a QA/QC proto-
col was developed to evaluate the accuracy of plant
identification. In this technique, a random sample
of 10% or 10 specimens (whichever was greater)
of the total number of species identified at the site
was collected for subsequent confirmation by a bo-
tanical expert. Prior to the vegetation survey, a ran-
dom numbers table was used to generate 10, 2-
digit (01,02, etc.) nonrepeating numbers within the
range of 1 to 30 (30 was the maximum value be-
cause 30 plots were sampled at each site). These
numbers identified the quadrats in which quality
control samples were to be collected.
Following identification of all species in the quad-
rat selected in step 1, a second two-digit random
number within the range of the number of species
found in that quadrat was generated and used to
select a voucher sample. For example, if there were
10 species found in the quadrat, a number between
1 and 10 was generated. If that number was 3,
then a voucher specimen of the third species en-
countered in that quadrat was collected. This was
done even if the species selected was very com-
mon and easy to identify, such as Typha latifolia.
Accuracy (defined as the closeness of a measured
value to the true value) was assessed by comparing
the confirmed species identifications to the identifi-
cation given by the field personnel such that:
% accuracy = (number of species correctly
identified in field/total number
confirmed)* 100
DATA ANALYSIS
VEGETATION-BASED METRICS
Vegetation metrics can be organized into three
groups based on the general level of biological or-
ganization they reflect: community-based metrics,
metrics based on plant functional groups, and spe-
cies-specific metrics. All should be developed on
the basis of field data collection and interpretation
of those data. In general, there are many more
potential metrics (i.e., plant community attributes)
than those that show strong biological signal. In all
cases, there should be an ecological understanding
as to why the metric works or does not (i.e., does it
make ecological sense). Unfortunately, not all metrics
are likely to work across all wetland types; there-
fore metrics must be tested when they are used in a
new wetland class (Keddy et al. 1993).
12
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A useful prelude to data analysis may include con-
sulting existing information on the autecology of
wetland plant species. One source of information
on species-specific tolerances is the EPA's National
Database of Wetland Plant Sensitivities (Adamus
and Gonyaw 2000). This database documents pub-
lished, species-specific assessments of plant toler-
ances, sensitivity, and general response to stressors,
particularly nutrient enrichment and hydrologic al-
teration. Responses are qualified by season and
plant life stage whenever such information is avail-
able. Limited information is also presented on the
tolerance of plant species to increased salinity, sedi-
mentation, and other stressors, but no attempt was
made to comprehensively compile literature on those
topics. The database summarizes information from
more than 200 sources on 1,082 plant species
(about 16% of all U.S. wetland plant species) and
450 nonwetland species that occur in the United
States. Little or no information has been published
for the remaining 84% of wetland plant species.
COMMUNITY METRICS
Extensive ecological literature describes the
changes in plant community composition in response
to human disturbance. For instance, there are pre-
dictable changes in plant species diversity, as well
as in the types of species that remain (Tilman 1999).
Guilds or functional groups, defined as groups of
species sharing certain traits predisposing them to
perform similar functions or respond in similar ways
to human disturbance, can be created to indicate
environmental change in wetlands (Hobbs 1997).
Well-known guilds in wetlands include carnivorous
plants, submerged aquatic species, or species tol-
erant of high sediment loads (Keddyetal. 1993,
Wardrop and Brooks 1998). An increase in non-
native invasive species is also considered to indi-
cate ecosystem change that may be due, for ex-
ample, to nutrient enrichment (Ehrenfeld 1983,
Thompson et al. 1987). These types of biological
signals can provide reliable information on the con-
dition of the wetland.
Human activities that alter natural hydrologic re-
gimes (changes to water quantity, water level fluc-
tuations, or water quality) have well-documented
impacts on plant communities (Ehrenfeld 1983, Vitt
and Bayley 1984, Ehrenfeld and Schneider 1991,
Wilcox 1995). Plant zonation patterns may shift,
species tolerant of human disturbance may invade,
or woody species may invade or die back as a re-
sult of drainage or flooding. Some of the responses
by the plant community that occur as a result of
hydrologic change include the following (from
Wilcox 1995, Ehrenfeld 2000):
• Decrease in species richness
• Possible decline of mutualistic interactions, such
as with pollinators or mycorrhizal fungi
• Absence of species that are sensitive to human
disturbance
• Increase in the numbers and dominance of in-
vasive and exotic species, such as Typha
angustifolia andLythrum salicaria
• Vegetation that is dominated by one species
(monospecific) or of one structural type
• Presence of either very dense or sparse stands
of vegetation (e.g., in response to water levels
that were stabilized at either lower or higher
than normal levels).
Floristic quality assessment index
The Floristic Quality Assessment Index (FQAI)
is a vegetative community index based on the
method developed for the Chicago region by
Wilhelm and Ladd (1988). It has been tailored
specifically to Ohio flora (Andreas and Lichvar
1995) and the flora of Michigan (Herman et al.
1996). Other States and regions are currently de-
veloping FQAI lists. The FQAI was originally de-
signed to assess the degree of "naturalness" of an
area based on the presence of ecologically conser-
vative species. It is thought to reflect the degree of
human disturbance to an area by accounting for the
presence of cosmopolitan, native species, as well
13
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as normative taxa. This index is capable of measur-
ing ecosystem condition because it assigns a re-
peatable and quantitative value to vegetation com-
munity composition. Use of the index requires that
local flora be available with coefficients of conser-
vatism assigned to each species. These lists are
finalized for some areas (e.g., Ohio, Michigan) and
under development in many others (e.g., Florida).
To calculate the FQAI, a species list is compiled
for the site. Then each species on the list is as-
signed a rating (tolerance values) of between 0 and
10 (Andreas and Lichvar 1995). A rating of 0 is
given to opportunistic native invaders and norma-
tive species. Tolerance values of 1-10 are assigned
as follows:
• Values of 1-3: applied to taxa that are wide-
spread and do not indicate a particular com-
munity
• Values of 4-6: applied to species that are typi-
cal of asuccessional phase of some native com-
munity
• Values of 7-8: applied to taxa that are typical
of stable or "near climax" conditions
• Values of 9-10: applied to taxa that exhibit high
degrees of fidelity to a narrow set of ecological
parameters.
The total species list from each wetland is used to
calculate the FQAI value for each site as follows:
I = R/N(Nl/2)
where I = the FQAI score, R = the sum of the
tolerance values (C of C) for all species at the site,
and N=the total number of native species.
The FQAI has been shown to respond to human
disturbance (see Appendix B) as well as wetland
functions such as biomass production (Figure 4) and
decomposition.
Other community-based metrics that we have
found useful, at least in some wetland classes are
shown in Table 1. This list provides a starting point
for investigating characteristics of the plant com-
munity and their response to human impacts.
Plant functional groups or guilds (also see
Appendix D)
Guilds can be defined as a group of species that
share similar traits or responses to human distur-
bance, although they may not be closely related
species. There are many ways in which wetland
species can be grouped on the basis of their role in
ecosystem function or their response to environ-
mental variables (Hobbs 1997, 1992). The cre-
ation of functional groups is a means to investigate
environmental change at the scale of the ecosystem
or landscape, and is based on the fact that set groups
of species will respond in similar ways to similar
types of stressors (e.g., hydrologic change, high
sediment loads). The creation of functional groups
then, is often based on nonphylogenetic groupings
(Gitay and Noble 1997). Some suggestions defin-
ing functional groups include:
• Perennials
• Annuals
• Sediment tolerant species
• Species tolerant of hydrological alterations
• Species tolerant of elevated nutrient levels
• Use of the tolerance values ("coefficients of
conservatism") provided in schemes like the
FQAI to establish "very tolerant" or "very in-
tolerant" groups
• C3 versus C4 species
• Submersed aquatic species
• Species that form persistent standing litter (see
Appendix C).
Responses of the wetland plant community to land-
scape change (including fragmentation of habitat)
14
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CD
§
40
38 -
36 -
34 -
32 -
30 -
28 -
26 -
24 -
22
20
18
16 1
14
12
10
0
500 1000 1500
Biomass (g/mA2)
2000
FIGURE 4: RELATIONSHIP BETWEEN FQAI SCORES AND BIOMASS PRODUCTION
(G-M2) IN EIGHT CENTRAL OHIO EMERGENT WETLANDS (FENNESSY ET AL. 1998).
have been observed in certain guilds such as sub-
mersed and emergent plant species. For example,
Lopez et al. (in press) found that wetland patch size
(defined as the size of the habitat fragment that con-
tained the study wetland) and the distance to neigh-
boring wetlands were positively correlated with the
diversity of some plant guilds (e.g., submersed her-
baceous plant species). The FQAI has also been
shown to respond to fragmentation (Figure 4).
Chapin (1991) specifically suggests using plant
guilds, such as plants that live in nutrient-poor soil
conditions, because particular traits might be strong
indicators of a stressful environment (e.g., a slow
growth rate, low photosynthetic rate, low capacity
for nutrient uptake, or specific hormone levels).
Chapin (1991) also reports that guilds made up of
longer-lived species may be a good indicator of
chronic ecosystem stress, whereas an "annual-plant
guild" maybe a good indicator of acute ecosystem
stress. That is, relatively longer-lived wetland plants
may have longer response times to environmental
stressors than shorter-lived plants and may be bet-
ter indicators of historic landscape change.
Species-specific attributes
Some species-specific traits also can be indica-
tive of wetland integrity, for example:
• Dominance of an early successional species
(e.g., Salix exigua)
• Metrics based on the health of individual plants
(e.g., Miller etal. 1993).
Attributes that do not seem to work
Some attributes do not give consistent, ecologi-
cally meaningful indications of wetland integrity.
Although they may be worth investigating on a
case-by-case basis, our experience has shown the
following:
15
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Potential metric
Alien taxa
Annuals
Carex species
Clonal growth
Dominant species (1, 2, or 3)
Decomposition (persistent standing litter)
Dicots
Dominance of Typha (or other taxon)
Early successional species
Facultative wetland taxa
Grasslike taxa (sedges, rushes, grasses)
Intolerant or sensitive taxa
Invasive taxa
Monocarpic taxa
Monocots
Native taxa
Nonvascular plant taxa
Number of plant guilds
(emergent, submerged, etc.)
Obligate wetland taxa
Perennials
Perennial to annual species (ratio)
Indicator species (e.g. Utricularia)
Species richness
Species tolerant of hydrologic change
Taxa in selected families (composites, etc)
Taxa richness by growth form (trees shrubs)
Taxonomic composition by strata
Tolerant or insensitive taxa (opportunists)
True aquatic species (floating, submerged)
Upland species
Vascular plant taxa
Woody species
Community similarity to reference
Wetness
Flood tolerance
Salinity tolerance
Scoring methods
Number of taxa Percent cover
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
zz
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
7
XX
XX
zz
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
XX
Predicted response
to human disturbance
increase
increase
decrease
increase
increase
increase
increase (?)
increase
increase
variable
decrease
decrease
increase
increase
decrease (?)
decrease
decrease
decrease
variable
decrease
decrease
decrease
decrease
increase
variable
decrease?
increase
decrease
variable
decrease
decrease
decrease
variable
variable
variable
Where
developed, tested
Mid-Atlantic
MN
MN, mid- Atlantic, New England
MN.Ohio
MN, New England
MN
OH
mid-Atlantic, New England
MN
Mid-Atlantic
MN
MN
New England
Mid-Atlantic
Mid-Atlantic
MN
Mid- Atlantic, New England
New England, OH
MN, mid-Atlantic
mid-Atlantic
MN
mid-Atlantic
New England
New England
New England (freshwater only)
New England (saltwater only)
Note: "?" indicates uncertainty about the response of that particular metric.
-------
Forest canopy species are often limited in the
signal they can provide because of their rela-
tively long response time.
Some doubt prevails as to the utility of metrics
that are based on the health of individual plants.
For example, chlorosis (yellowing or turning
brown) in plant leaves may indicate stress to
that individual; however, this condition may also
result from many naturally occurring phenom-
ena such as aging. Quantifying individual health
has worked well in othertaxonomic assemblages
(e.g., fish and the presence of tumors), but no
clear dose-response pattern has been seen with
regard to plants.
Although there has been some interest in the
potential of the wetland plant indicator status
classification system developed for conducting
delineations (U.S. CoE 1997) to interpret veg-
etation patterns, there is concern about using
the indicator status to evaluate integrity. The
wetland indicator status describes the probabil-
ity that a given plant species will occur in wet-
lands. The ratings are found in the "National
List of Plant Species that Occur in Wetlands"
(Reed 1997,1988); within it is alist of the indi-
cator status of all wetland plants known to oc-
cur in U.S. wetlands. Currently about 7,000
species are on the list, each of which has been
assigned an indicator status for the regions in
which it occurs. Each species is assigned one
of four indicator status categories based on the
probability that the species will be found in a
wetland. These are obligate wetland (OBL),
facultative wetland (FACW), facultative (FAC),
and facultative upland (FACU). Obligate spe-
cies occur in wetlands more than 99% of the
time, whereas facultative species are just as
likely to be found in uplands (50% of the time)
as in wetlands (50% of the time). Species not
found on the list are considered to be obligate
upland (UPL) species. The indicator status was
not designed to provide information on the con-
dition of a wetland. Many obligate (OBL) wet-
land species, for instance, are invasive and/or
normative species (Typha sp., for example), and
so do not provide an indication of "integrity" in
this way.
• In general, care should be taken because metrics
that are useful in one class of wetland (e.g.,
emergent) are not necessarily transferable to
other classes.
LIMITATIONS OF
CURRENT KNOWLEDGE-
RESEARCH NEEDS
TJTetland vegetation promises to be one of the
Y V best indicators for use in assessing the bio-
logical integrity of wetlands. The examples given in
Appendixes A-D provide illustrations of how these
techniques are currently being tested and used in
different State programs. However, research on
the relationship between environmental conditions
and the response of different species could provide
greater sensitivity and precision in detecting impair-
ment. For instance, methods are lacking for char-
acterizing the role of the landscape surrounding a
wetland. Questions such as the effect of surround-
ing uplands on determining the site's condition are
difficult to answer. Land use in a wetland's water-
shed largely determines the quantity and quality of
water that enters the site, and this has obvious re-
percussions for the composition of biotic commu-
nities. In addition, human disturbance can be mani-
fest at many scales; how can we assess these and
begin to be truly diagnostic about the stressors that
lead to a loss ofbiological integrity? Sampling tech-
niques are sometimes lacking as well. For instance,
small wetlands are sometimes undervalued, particu-
larly when they occur in a mosaic of wetland patches
interspersed with another habitat type. Useful tech-
niques to evaluate wetlands in this landscape con-
text are needed.
17
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There are also limits on the use of methods that
have already been developed (e.g., the FQAI) be-
cause we lack the information needed to apply them
to wider geographic areas. There is a need to cre-
ate plant response guilds and/or FQAI tolerance
lists for more regions of the country. In fact, de-
spite the vast literature on wetland vegetation, the
majority of wetland plant species have not been stud-
ied at all, so there is no literature to consult on the
autecology of many species. One remedy is to ini-
tiate studies on the dose-response relationship, both
in the field and in the lab or greenhouse, between
different plant species and different types of stres-
sors. This would do much to provide information
on species for which little is known.
18
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22
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APPENDIX A
A TALE OF Two METHODS: DEVELOPING, EVALUATING, AND
CHANGING SAMPLING METHODS
JOHN J. MACK AND M. SIOBHAN FENNESSY
The Ohio Environmental Protection Agency be-
gan evaluating vegetation sampling methods in 1996.
Major concerns in selecting a sampling method were
ease of use, cost, reproducibility of results, and ob-
taining as complete a list plant species at a wetland
as possible. This last concern related to Ohio's use
of a Floristic Quality Assessment Index (FQAI)
(Wilhelm 199X; Andreas and Ladd 1995) which
requires a relatively complete flora of a site.
Ohio EPA sampled disturbed and undisturbed
wetlands in western and central Ohio in 1996 and
1997. Initially, Ohio EPA adopted a fixed transect
method with 1m2 and 10m2 circular nested quad-
rats spaced evenly along the transect. A minimum
of 30 quadrats were sampled along 3 transects
(30m2 area sampled herbaceous vegetation and
300m2 woody vegetation), with at least one transect
oriented perpendicular to the other two (hereafter
transect-quadrat method). In addition, plants lo-
cated outside the quadrats but within a 5m wide
"belt" along the transect were identified but no den-
sity or dominance information was recorded for
these plants (hereafter transect-belt method). Within
the quadrats, percent cover, stem counts and DBH
(woody only) were recorded for each species.
As Ohio EPAIBI development advanced, it be-
come apparent that many successful attributes were
associated with measures of dominance, including
percent cover and density (stems/ha). However,
using the existing method, 30%-60% of the plants
observed had only presence/absence data associ-
ated with them (Figure A-1). Other issues included
(1) the size of the area sampled to characterize
forested communities was too small. The forestry
literature recommends 400-1,000 m2 as minimum
area to adequately characterize eastern forest com-
munities (Peet et al. 1998); (2) a perceived over
sampling of species at the wetland edges.
In 1999, Ohio EPA reevaluated its sampling
method and adopted a flexible, multipurpose releve
method used by the North Carolina Vegetation Sur-
vey (hereafter releve method) as described in Peet
et al. (1997,1998). This method can be used to
sample such diverse communities as grass and forb
dominated savannahs, dense shrub thickets, forest,
and sparsely vegetated rock outcrops and has been
used at over 3 000 sites for over 10 years as part of
the North Carolina Vegetation Survey. It is appro-
priate for most types of vegetation, flexible in inten-
sity and time commitment, compatible with other
data types from other methods, and provides infor-
mation on species composition across spatial scales.
It also addresses the problem that processes af-
fecting vegetation composition differ as spatial scales
increase or decrease and that vegetation typically
exhibits strong autocorrelation (Peet et al. 1998).
The method employs a set of 10, 10 xlOm sam-
pling units in a 20 x 50 m layout. Within the site to
be surveyed, a 20 x 50 m grid is located such that
the long axis of the plot is oriented to minimize the
environmental heterogeneity within the plot. In ef-
fect then, the method proposed by Peet et al. in-
corporates the use of releves found in the Braun-
Blanquet methodology in as much as the length,
width, orientation, and location of the modules are
qualitatively selected by the investigator based on
site characteristics; however, within the modules,
23
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1.0 H
0.8 -
tn
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I?
M— CD
0 >
c o
g O
I!
o £
0.6 -
0.4 -
0.2 -
0.0 -I
I
gahanna
I
leafy oak
I
mishne
FIGURE A-l: PROPORTION OF PLANT SPECIES THAT WERE MISSING DOMINANCE
OR DENSITY DATA AT THREE WETLANDS RESAMPLED IN 1999 USING RELEVE
METHOD AS DESCRIBED IN PEET ET AL. (1998).
standard quantitative floristic and forestry informa-
tion is recorded, e.g. density, basal area, cover, and
soon.
Ohio EPAresampled several wetlands with the
new method that had previously been sampled with
transect-quadrat method. These wetlands included
a highly degraded emergent marsh, a sparsely veg-
etated vernal pool, and a floristically rich forested
wetland. The releve method solved many of the
problems listed above. For instance, all plants have
cover or density data associated with them. How-
ever, at all three sites, the original transect-belt
method had the highest species list (highest species
richness) (Figure A-2); this difference was most
apparent in the degraded to moderately degraded
sites (Mishne and Gahanna). At the floristically rich
wetland (Leafy Oak), the difference between the
releve method and the transect-belt method was
11 species, or 14% of the maximum value (Figure
A-2). At the highly degraded emergent marsh
(Mishne), substantially greater numbers of species
(80% more) were identified using the transect-quad-
rat and transect-belt methods than the releve
method. These tended to be upland or facultative
species growing in a band around the edge of the
wetland. Inclusion of these plants raised the Floris-
tic Quality Assessment Index scores for the Mishne
site (Figure A-3). Species richness was 48% higher
at the Gahana Woods site using the quadrat + belt
method as it was using the releve method. But, at
the floristically rich Leafy Oak site, the releve
method performed nearly as well as the transect-
belt method with regards to FQAI scores (Figure
A-3). Using the quadrat only data from the transect-
quadrat methods yielded very similar scores to the
releve method.
A comparison of vegetation IBI scores of data
from the transect-quadrat and releve methods
yielded very similar results: the relative position of
the wetlands sampled using both methods did not
significantly change (Figure A-4).
24
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-------
The releve method has several advantages over
transect-quadrat methods: (1) it allows for an easy
qualitative stratified sampling of the dominant plant
communities; (2) it provides a more complete for-
est inventory; (3) the quantitative data is
intercomparable with other standard vegetation sam-
pling methods; (4) it is relatively quick (1-3 sites
per day with an experienced team); it is easily
adaptable to unique situations and shapes of com-
munities (the module system allows you to build up
or down in plot size); (5) it provides the data for
phytosociological analysis; and (6) it ensures all
plants identified have dominance data associated
with them.
The releve does not allow mapping of the vegeta-
tion communities like a fixed transect method would
and the releves are often difficult to lay out in dense
shrub communities. In addition, the flora of the
wetland is somewhat less complete using the releve
method, although this could be compensated for by
doing a qualitative survey outside of the plot (some-
thing Ohio EPA does as a qualitative check on the
appropriateness of the plot location). Cover data
would again be lacking however.
In sum, both the transect-quadrat and releve
methods yielded equivalent results when the data
resulting from these methods was used to calculate
a vegetation IBI. Thus, the conclusion may be that
whatever method is selected, that it be capable of
sampling with sufficient completeness such that hu-
man disturbances are detectable.
o
§
"Z
O)
++
1 1 1 1 1 1 1 1
10 20 30 40 50 60 70 80
• gahanna 97
® gahanna 99
O leafy oak 97
> leafy oak 99
I> mishne 97
$ mishne 99
+ other
high disturbance low disturbance
low integrity high integrity
FIGURE A-4: INTERIM VEGETATION INDICES OF BIOLOGICAL INTEGRITY (IBI)
SCORES FOR 45 WETLANDS IN THE EASTERN CORNBELT PLAINS ECOREGION
OF OHIO. COMPARISON OF IBI SCORES FOR THREE WETLANDS
SAMPLED USING TRANSECT-QUADRAT AND RELEVE METHOD.
DATA FROM MACK AND MICACCHION (IN PREP).
26
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APPENDIX B
DEVELOPMENT OF VEGETATION IBIS:
THE OHIO EXPERIENCE AND LESSONS LEARNED
JOHN J. MACK, OHIO ENVIRONMENTAL PROTECTION AGENCY
The State of Ohio has well-developed biological
criteria (or biocriteria) for streams, e.g., the Inver-
tebrate Community Index (macroinvertebrates), In-
dex of Biological Integrity (fish), and Modified In-
dex of Well Being (fish) (Yoder and Rankin 1995).
These indices are codified in Ohio Administrative
Code Chapter 3745-1. Until recently however,
surface waters of the State that are jurisdictional
wetlands were only generically protected under
Ohio's water quality standards. On May 1,1998,
the State of Ohio adopted wetland water quality
standards and a wetland antidegradation rule (OAC
Rules 3745-1-50 to -54). These wetland quality
standards developed narrative criteria for wetlands
and created the "wetland designated use."
Ohio began development of sampling methodolo-
gies and began sampling reference wetlands for
biocriteria development in 1996. To date, Ohio
has sampled nearly 60 wetlands located primarily
in the Eastern Cornbelt Plains Ecoregion located in
central and western Ohio. This work has been
funded since 1996 by several different U.S. EPA
Region 5 Wetland Program Development Grants
including CD995927, CD995761, CD985277,
CD985276, CD985875, and CD975350.
The first two years of data laid the groundwork
for standardizing sampling methodologies, classify-
ing wetlands, identifying potential attributes, and
developing metrics using vascular plants, amphib-
ians, and macroinvertebrates. The wetlands stud-
ied have included depressional emergent, forested,
and scrub-shrub wetlands, floodplain wetlands, fens,
kettle lakes, and seep wetlands. The wetlands be-
ing studied span the range of condition from highly
disturbed to relatively undisturbed, i.e., "reference"
conditions.
Based on the results to date (see Fennessy et al.
1998a, b; Mack et al. 2000, Mack in prep.), Ohio's
research supports the use of vascular plants as taxa
group for wetland biocriteria (Figure B-l).
Successfiil attributes for emergent wetlands include
floristic quality assessment index (FQAI) score (see
below), ratio of shrub species to total species, num-
bers of Carex spp., numbers ofdicot spp., num-
bers of plants with facultative wet (FACW) or ob-
ligate (OBL) wetland indicator status, heterogene-
ity (Simpson's Index), standing biomass, and rela-
tive cover of tolerant and intolerant plant species,
where "tolerance" is determined by the plant's "co-
efficient of conservatism," which is derived from a
State or regional FQAI system. In addition, for
wetlands dominated by woody species, relative den-
sity of shrubs and small trees and tree size class
equitability have also proved to be useful attributes.
Semiquantitative disturbance/integrity scales
Ohio EPA has had good success in developing a
Semiquantitative disturbance/biological integrity
scale called the Ohio Rapid Assessment Method
for Wetlands v. 5.0 (Mack2001, Figure B-2). Until
such time as more quantitative variables like per-
cent impervious surface are found, this type of tool
is a good candidate for the problematic x-axis in
27
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• f •
;/ * .
* •
•
X
• • *
o coastal
+ flats
x fringing
# impndmnt
• isol deprs
* ripar deprs
A ripar hdwtr
ffl slope isol
S slope ripar
1 1 1 1 1 1 1 1 1 1 1
0 10 20 30 40 50 60 70 80 90 100
high disturbance low disturbance
low integrity high integrity
FIGURE B-l: VEGETATION INDEX OF BIOLOGICAL INTEGRITY SCORE BY
HYDROGEOMORPHIC CLASSIFICATION FOR N = 65 FORESTED AND EMERGENT
WETLANDS IN THE STATE OF OHIO.
"Coastal" Lake Erie coastal marsh, "flats" isolated flats wetlands, "fringing" wetlands fringing natural lake
other than Lake Erie, "impoundment" -wetlands located in or formed by human impoundment, "isol
depr" isolated depressional wetland, "ripar depr" depressional wetland located in a riparian context, "ripar
hdwtr" wetland located next to or near 1 or 2 order stream, "slope isol" slope wetland in isolated landscape
position, "slope ripar" slope -wetland in riparian landscape position.
wetland biocriteria development. See also Carlisle
et al. (1999), where a similar system was used to
rank levels of disturbance.
Classification
Classification is definitely an iterative process. In-
vestigators should definitely consider a
hydrogeomorphic (HGM) classification scheme if
one has been developed for their region of interest,
at least as a starting point. For example, shrub domi-
nated wetlands began to emerge as a separate class
only after several years of sampling. However, the
experience in Ohio suggests that grosser classes
based on dominant vegetation (emergent, scrub-
shrub, forested, etc.) may work also. A goal of a
cost-effective biocriteria program is to have the few-
est classes that provide the most cost-effective feed-
back. With vegetation, data from Ohio are sug-
gesting somewhat diverse wetland types may be
"clumpable," since even though their floras are dif-
ferent at the species level, the quality/responsive-
ness of their unique floras to human disturbance is
equivalent (Figure B-2). This is also a concern in
States with high degrees of wetland loss where two
few wetlands of a particular HGM class remain to
analyze as a separate class.
Floristic quality assessment indexes
Ohio EPAhas found that Floristic Quality Assess-
ment Index (FQAI) scores and subscores are very
successful attributes and metrics for detecting dis-
turbance in wetlands (Figures B-4 and B-5) (see
Andreas and Lichvar 1995, Herman et al. 1996,
Wilhelm and Masters 1995).
28
-------
Category using biologically calibrated ORAM score
category 1 category 2 category 3
100 -
90 -
80 -
70 -
CD 60 —
o 50 -
m 40 -
30 -
20 -
10 -
0 —
h>
.
•
' * *"
3
0 10 20 30
modified £
cat 2 cat 2 °
<> « > g
I •£
1 • ^
^
1
1
1
I
1
- - m6
40 50 60
ORAM v5.0 score
u * •
*
5 1 1 1 1
70 80 90 10C
N
S
\
/
^
JS
)
> category 3
'category 2
o
O
•5
12
• category 1
FIGURE B-2: RELATIVE COVER OF TOLERANT HERB AND SHRUB STRATUM SPECIES
FOR REFERENCE (LEAST IMPACTED) AND NONREFERENCE (ALL OTHER SITES) OF
FORESTED AND EMERGENT WETLANDS (N = 65) FOR FOUR ECOLOGICAL REGIONS
IN THE STATE OF OHIO.
E emergent, F forested, SS scrub-shrub.
95-
85-
75-
m
> 65-
55-
45-
HGMCode
Boxplots of VIBI by HGM Code
(means are indicated by solid circles)
FIGURE B-3: Box AND WHISKER PLOTS OF EMERGENT AND FORESTED
REFERENCE (LEAST-IMPACTED) WETLANDS (N = 29).
SEE FIGURES B-5 AND B-6 FOR HGM CODE DESCRIPTIONS.
29
-------
1.0 -
0.9 -
0.8 -
0.7 -
£ 0.6 -
0> 0.5 —
| 0.4 -
0.3 -
0.2 -
0.1 -
0.0 -
o
o
** G
« 0
* D
0 t
* 00 0 "
°o oV '.. .1
x G 0 0 Q A «
1 1 1 1 1 1 1 1 1
10 20 30 40 50 60 70 80 90
« non-ECBP
n non-EOLP
x non-WAP
» ref-ECBP
• ref-EOLP
* ref-HELP
A ref-MIDP
ORAM v5
FIGURE B-4: RELATIVE COVER OF TOLERANT HERB AND SHRUB STRATUM
SPECIES FOR REFERENCE (LEAST IMPACTED) AND NONREFERENCE
(ALL OTHER SITES) OF FORESTED AND EMERGENT WETLANDS (N = 65) FOR FOUR
ECOLOGICAL REGIONS IN THE STATE OF OHIO.
ECBP Eastern Corn Belt Plains, EOLP Erie-Ontario Lake Plains, HELP Huron-Erie Lake Plains,
MIDP Michigan-Indiana Drift Plains, WAP Western Allegheny Plateau.
40 -
30 -
20 H
10 -
0 -I
ORAM v5
FIGURE B-5: FQAI SCORE BY HYDROGEOMORPHIC CLASSIFICATION FOR N = 65
FORESTED AND EMERGENT WETLANDS IN OHIO.
"coastal" Lake Erie coastal marsh, "flats" isolated flats wetlands, "fringing" wetlands fringing natural lake
other than Lake Erie, "impoundment" wetlands located in or formed by human impoundment, "isol
depr" isolated depressional wetland, "ripar depr" depressional wetland located in a riparian context, "ripar
hdwtr" wetland located next to or hear 1 or 2 order stream, "slope isol" slope wetland in isolated landscape
position, "slope ripar" slope wetland in riparian landscape position.
• <
8 • A «
+ . •• ,"* • •
• •
^ • • ^ya • •
• • • *
. * 8 * B '
• A • •
» *^
- ** ."* '* +*
m „
coastal
+ flats
0 fringing
X impdmnt
• isol depr
• ripar depr
A ripar hdwtr
v slope isol
8 slope ripar
I I I I I I I I I
10 20 30 40 50 60 70 80 90
3O
-------
Field and lab methods
After experimenting with both transect/quadrat and
releve-style plot methods, Ohio has adopted a plot
based method which allows for a qualitative strati-
fication of wetland by dominant vegetation com-
munities. This method appears flexible and adapt-
able to unique site conditions, provides dominance
data for all species in all strata, provides data
intercomparable with other common methods, is
relatively easy to learn, and is relatively fast and
cost-effective (up to 2 to 3 plots can be completed
in a day).
Whatever sampling method is adopted, it is es-
sential that dominance and density information
(cover, basal area of trees, stems per unit area, rela-
tive cover, relative density, importance values, etc.)
be collected. Many of the most successful attributes
Ohio has found in developing a vegetation IBI are
based on cover data of the herb and shrub layers
and density data of the shrub and tree layers.
Definitely consider using cover classes in general
and a class scheme that works on a doubling prin-
ciple to aid in consistent inter-investigator usage.
Then use the midpoints of the class for your analy-
sis. This seemed to help with consistent usage and
smoothing out the roughness in cover data.
31
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APPENDIX C
MINNESOTA INDEX OF VEGETATIVE INTEGRITY (IVI)
MARK GERNES
Minnesota intends to use its biological assessment
results to report on and track wetland conditions
within local watersheds. This assessment tool will
be useful for evaluating best management practices
for wetlands and wetland restorations, and priori-
tizing wetland-related resource management deci-
sions. Minnesota also intends to develop numeric
wetland biological criteria.
Minnesota has proposed 10 wetland vegetation
metrics that have been combined into a multimetric
"Index of Vegetative Integrity" (IVI). Two metrics
focus on taxa richness, four are based on life-form
guilds, two are sensitive and tolerant taxa metrics,
and two are community-structure metrics. This
multimetric index has been used effectively in Min-
nesota to assess wetland condition. A100 m2 releve
plot method was used to sample the vegetation. All
sampling was conducted in the wetland emergent
vegetation zone. Additional metrics may be devel-
oped as this work progresses. Reference sites were
chosen as least-impacted wetlands; stormwater run-
offer agricultural activities disturbed the other sites.
Scoring criteria for the metrics described below are
shown in Table C-l.
1. Vascular genera metric
Rationale: The vascular genera metric expresses
the richness of native genera occurring in the wet-
land (i.e., the number of native vascular plant gen-
era in a 100 m2 releve plot). Many genera have
some species that are native and others that are
nonnative. In these instances the genus is not ex-
cluded from the count. When all taxa within the
genus are not native to Minnesota the genus is not
counted. An example might be the genus
Echinochloa, where two common wetland spe-
cies occur. Echinochloa muricata is native
whereas E. crusgalli is not. When plants in this
genus occurred in the sampling plot but could not
be identified by species, they were counted. This
metric was developed for depressional wetlands,
so all taxa recognized as being nonwetland taxa
were excluded from the count. Species are identi-
fied as being nonwetland taxa in accordance with
the Region III assignment (Reed 1988) and include
those species with indicators of FAC+, FACU, and
UPL. This forces the resulting count to reflect the
wetland condition as opposed to the terrestrial and
aquatic community edge.
Although keeping this metric at the genus level will
make it accessible for less specialized biologists and
simplify the sampling, whenever possible all identi-
fications were done to the species level. Interest-
ingly, when this metric was developed it was based
on species-level identification. However, the ge-
nus-level scoring gave a stronger negative response
to human disturbance. Caution should be used in
applying this metric where plant diversity is natu-
rally low. Some examples of wetland plant com-
munities that have naturally low numbers of species
are lake sedge (Carex lacustris), fringe communi-
ties, wild rice (Zinniapalustris) beds, and hardstem
bulrush (Scirpus acutus) communities.
2. Nonvascular taxa metric
Rationale: The nonvascular taxa metric expresses
the number of nonvascular taxa including liverworts,
mosses, lichen taxa, and the macroscopic algae
Chora and Nitella. In the Minnesota study, the
maximum number of nonvascular taxa observed at
any site was two (including four of the six reference
sites). Six of the agriculturally impacted sites and
32
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TABLE CM: SCORING CRITERIA FOR VEGETATION-BASED METRICS IN THE
MINNESOTA IVI. THE NUMBER OF SITES SCORING AT EACH LEVEL is GIVEN, AS ARE
THE NUMBER OF LEAST-IMPACTED REFERENCE (REF) SITES.
Vascular plant metric
Genera Score
#Sites
.9-14
<9
5
3
1
Nonvascular plant metric
#Taxa Score
6 (3 ref)
10 (3 ref)
10
#Sites
>1 5
1 3
0 1
Carex cover metric
Total Carex Cove Score
>3 5
0.1-2.9 3
0 1
Grasslike species metric
#Species Score
>8 5
7-Feb 3
<2 1
6 (4 ref)
7 (2 ref)
11
#Sites
6 (2 ref)
11 (4 ref)
9
#Sites
4(2 ref)
6(4 ref)
12
Monocarpic species metric
Cover Score #Sites
Aquatic guild metric
Species Score
#Sites
>6
.3-6
<3
Sensitive taxa
5
O
1
metric
Sensitive species Score
>4
.1-3
0
Tolerant taxa
Tolerant Taxa
< 25%
25.1 -60%
> 60%
5
3
1
metric
Score
5
3
1
5 (4 ref)
9 (2 ref)
12
#Sites
3 (ref)
12 (3 ref)
9
#Sites
4 (4 ref)
12 (2 ref)
10
Dominance metric
Dominance
<0.07
0.08-0.2
>0.2
Score
5
3
1
#Sites
7 (3 ref)
11 (3 ref)
8
Persistent litter metric
Cover Score
#Sites
>2.5
2.0-2.49
<2.0
5
3
1
7 (4 ref)
11 (2 ref)
8
< 25%
25 - 75%
> 75%
5
3
1
6 (4 ref)
13 (2 ref)
7
Note: AH data were collected in 100 m2 releve plots.
five of the stormwater sites supported no
nonvasculartaxa. This was not surprising. Blindow
(1992) reported that charaphytes and possibly other
nonvascular taxa are more sensitive to eutrophica-
tion than are angiosperms. It is likely this metric
could be strengthened by improving the level of iden-
tification for the mosses, bringing them to genus.
3. Carex cover metric
Rationale: The Carex cover metric was calcu-
lated by summing the cover class for all Carex taxa
sampled in each plot. Carex occur as an important
structural component of shallow wetland emergent
plant communities. Members of the Carex genus
are particularly common in shallow marsh and wet
33
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meadow communities (Eggers and Reed 1997).
They are known to be adversely affected by such
environmental stressors as excessive siltation and
hydrologic alteration and nutrient enrichment
(Wilcox 1995). Field observations have demon-
strated that these plants are among the most sensi-
tive to human disturbance and among the first to
either disappear from sites or be poorly recruited in
most wetland restoration projects (Galatowitsch
1993).
In order to receive a score of 5 a site must sup-
port at least 25% Carex cover. It is interesting that
two agricultural, two stormwater, and two refer-
ence sites had a score of 5 for this metric. These
sites were likely influenced by groundwater that may
have positively influenced the amount of Carex they
supported.
4. Grasslike species metric
Rationale: The grasslike species metric expresses
the richness of grasses (Poaceae), sedges
(Cyperaceae), and rushes (Juncaceae). Structur-
ally these plants are very similar and generally oc-
cupy similar niches. Only native taxa in these three
families were tabulated for the grasslike species
metric. This metric is important because native taxa
in these plant families are frequently among the first
to begin decreasing following human disturbance
(Wilcox 1995). Galatowitsch (1993) reports that
particularly the sedges are poorly recruited in wet-
land restoration projects, which suggests sedges
have a relatively low ecological tolerance to stress.
There was only a moderate (R2 = 0.43) linear re-
lationship between the number of grasslike species
and the gradient of human disturbance. The statisti-
cal analysis showed this metric to be one of the
weaker plant metrics. However, this measure was
able to distinguish the severely impaired sites from
the reference sites.
5. Monocarpic species metric
Rationale: Monocarpic species flower only once
in their life cycle and typically including annual and
biennial species. We used a mathematical function
to relate the importance of monocarpic species (us-
ing cover) at each study site, van der Valk (1981)
reported that changes in water level through natural
drying or inundation can result in habitat changes
that facilitate the growth of monocarpic species. We
calculated this metric as a sum of the monocarpic
species richness and cover class values divided by
the monocarpic species cover. Only native mono-
carpic taxa were not included because nonnative
monocarpic taxa are often aggressive and could
skew the response.
The monocarpic species metric responded
strongly to hydrologic fluctuations. As such it would
be useful to include this metric in a multimetric in-
dex to respond to signals from worst-case sites,
particularly those affected by severe hydrologic fluc-
tuations.
6. Aquatic guild metric
Rationale: This metric evaluates the number of
aquatic guilds at a site. Submerged plants, either
rooted or unrooted, and floating vascular plants such
as the duckweeds are life-form-dependent aquatic
macrophytes that comprise the aquatic guild used
in this metric. The guild is adapted from
Galatowitsch and McAdams (1994), who recog-
nize six separate aquatic guilds. Four of their guilds,
including "rooted submersed aquatics," "unrooted
submersed aquatics," "floating perennials," and
"floating annuals" were used in constructing this
metric. When counting the number of aquatic guild
species only native species were included. The
aquatic guild taxa were expected to be most re-
sponsive to the water quality. Though the relation-
ship between this metric and the human disturbance
index was not a statistically significant linear rela-
tionship (R2 = 0.28), the data show a separation of
34
-------
the a priori reference sites from more than half of
the degraded sites. This metric appears to be par-
ticularly important in larger or more open wetlands.
7. Sensitive taxa metric
Rationale: The sensitive taxa metric evaluates the
decrease in richness of taxa that are most suscep-
tible to human disturbance. To determine which
species were sensitive, a matrix of all plant taxa
sampled in the project was created by site. In this
matrix the a priori reference sites were arranged
along one side of the site continuum. Taxa either
unique to the reference sites or those that occurred
in two or more reference sites and only one im-
paired site were considered to be sensitive. Any
nonnative species meeting these criteria were not
considered to be sensitive taxa. The list of taxa
considered to be sensitive included Asdepias
incarnata, Dulichium arundinaceum,
Eriophorum gracile, Scirpus validus, and Iris ver-
sicolor. Recognition of tolerant taxa was based
partly on reported responses of plant species to
human disturbance (Wilcox 1995, Rice and
Pinkerton 1993, Weisner 1993, Squires and van
der Valk 1992) as well as personal field observa-
tions in this project. All nonnative plant taxa were
also considered to be tolerant. Percent tolerant spe-
cies values were developed as a proportion of the
number of tolerant species in a sample divided by
the total number of all taxa in the sample. Possible
values for this metric range from 1 to 100. Our
results for this metric showed that from 11% to
100% of the taxa in the sample were tolerant. The
reference wetlands clearly had proportionately fewer
tolerant species than the impaired sites. This metric
gave the strongest response signal out of the 10
vegetation metrics.
8. Dominance metric
Rationale: The dominance metric incorporates the
distribution or concentration of cover class values
relative to the taxa richness for native species within
the sample. Used in this way it is similar to an ex-
pression of evenness. The formula for calculating
dominance was taken from Odum (1971) and ex-
pressed as:
D = (n/N)2
where a = the cover class for each taxa within an
emergent sampling plot, andN=the sum of all cover
class values for all taxa within the sampling plot.
The mathematical range of this fiinction is between
0 and 1, with a more biologically diverse wetland
scoring near 0 and more monotypic sites scoring
near 1. In this study, the eight most impaired sites
had higher dominance values than all the reference
sites. The dominance metric is considered to be
one of the moderately reliable metrics.
9. Persistent litter metric
Rationale: Persistent litter is defined as being re-
sistant to decomposition. It does not provide as
many available nutrients or as much detrital energy
to drive the wetland system as does readily decom-
posable litter. Decomposing litter provides micro-
habitats and nutritional benefit for many aquatic in-
vertebrates (Campeau et al. 1994). Scoring for
the persistent litter metric was based on a sum of
the abundance cover classes for plant taxa recog-
nized as having persistent litter, including: common
reed (Phragmites), bullrushes (Scirpus), smart-
weeds (Polygonum), cattails (Typha), and
burreeds (Sparganium). This measure proved to
be a reliable metric.
35
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APPENDIX D
TOLERANCE GROUPS OF WETLAND PLANTS FOR USE IN A PLANT-BASED
INDEX OF BIOLOGICAL INTEGRITY
DENICE HELLER WARDROP AND ROBERT P. BROOKS
PENN STATE COOPERATIVE WETLANDS CENTER
In preparation for a plant-based Index of Bio-
logical Integrity (IBI), individual plant community
metrics have been tested for robustness along a gra-
dient of human disturbance in Pennsylvania wet-
lands. Human activities of high interest in Pennsyl-
vania include land-use conversion to agricultural and
urban uses; dominant stressors associated with these
activities are hydrologic modification, sedimenta-
tion, and nutrient input. General measures of com-
munity response, such as species richness, diver-
sity, and evenness, when taken over all wetland
types, do little to establish general patterns of re-
sponse to these stressors. Suitable metrics for ex-
pressing plant community responses to disturbance
were not available and therefore needed to be con-
structed. One traditional metric is the use of func-
tional, or tolerance, groups of organisms. Existing
functional groups do not incorporate stress-resis-
tant characteristics. For example, currently-used
functional groups do not include traits that exem-
plify a species' germination capabilities as well as
its ability for clonal growth; both traits are basic to
a plant's ability to tolerate sedimentation. Toler-
ance groups of plants relating to sedimentation and
hydrologic stress were, therefore, constructed us-
ing field data on 70 reference wetlands in Pennsyl-
vania
mentation). Field data included presence/absence
and percent cover data, resulting in more than 500
plant species represented over approximately 800
plots. Characterization of plant communities in these
wetlands showed clear associations between indi-
vidual species and ability to tolerate sediment. The
tolerance for sedimentation in most species, how-
ever, is also dependent on other possible co-oc-
curring stressors, such as wetting and drying cycles.
HGM subclass was used as an indicator of levels
of these co-occurring stressors. It was expected
that the ability of a plant community or individual
species to occupy space along a gradient of increas-
ing sediment accumulation would be different for
various HGM subclasses. This was borne out in
shifts of some species between sediment tolerance
groups within wetlands of different HGM types, or
drastic reductions in the mean percent cover dem-
onstrated between HGM subclasses. In this con-
text, HGM classification was important in estab-
lishing the range of other co-occurring stressors,
and thus provided a constrained condition for ex-
amining the effects of sedimentation. Because of
the clear value of HGM classification as an orga-
nizing variable of co-occurring stressors, sediment
tolerance groups were established for each HGM
wetland type.
Our wetland sites were chosen to encompass six
common (HGM) subclasses of Pennsylvania's five
ecoregions (headwater floodplains, mainstem flood-
plains, slopes, riparian depressions, surface depres-
sions, and fringing), as well as high, moderate, and
low levels of impact from human activities (e.g., el-
evated sedimentation, nutrient loading, habitat frag-
Sediment tolerance groups were established by
tabulating average percent cover of individual spe-
cies, when present, with sedimentation levels. Spe-
cies were categorized as very tolerant, moderately
tolerant, slightly tolerant, and intolerant on the basis
of their association with environments of varying
sedimentation. In general, species that were cat-
36
-------
egorized as very tolerant or moderately tolerant in-
creased their percent cover (dominance) over the
disturbance gradient. For species that represented
either end of the disturbance gradient (i.e., highly
tolerant or intolerant), the appropriateness of the
plant species as an ecological indicator was assessed
utilizing calculation of validity and significance. Va-
lidity is ameasureofhow often an indicator (e.g., a
particular plant species) is found with whatever it is
expected to indicate (e.g., sedimentation category).
This is expressed as the ratio of the number of plots
where these two items occur together to the total
number of plots where the indicator occurs, ex-
pressed as a percentage. Significance denotes the
frequency with which the indicator and that par-
ticular object are associated. Significance is deter-
mined by expressing as a percentage the ratio of
the number of plots where both occur to the total
number of plots where the indicator object is found.
Validity and significance were calculated for each
plant species/sedimentation category combination.
Plant species were sorted according to validity and
significance values in each sedimentation category.
Hydrologic groups were determined in a similar
fashion, utilizing well monitoring data collected by
the Penn State Cooperative Wetlands Center at 27
sites. Hydrologic measures, based on water level
data recorded every 6 hours, were the following:
• Median depth to water
• Percent time water level was within the top 3 0
cm
• Percent time upper 3 0 cm was saturated, inun-
dated, or dry
• Percent time upper 10 cm was saturated, inun-
dated, or dry
Utilizing these data, sites were assigned to one of
five hydrologic groups, ranging from predominantly
inundated to predominately dry. Plant data from
406 plots, with a total of 187 plant species, was
used to construct the groups. Groups were estab-
lished by tabulating average percent cover of indi-
vidual species, when present, within each of the five
hydrologic groups. Wetland plant indicator status
(obligate, facultative, etc.) was an extremely poor
predictor of an individual species' associated hy-
drologic regime. For example, species found in
wetlands that were almost constantly inundated had
indicator statuses ranging from obligate to faculta-
tive-upland. However, HGM classification is a suit-
able surrogate for hydrologic regime, suggesting that
hydrologic groups can be constructed without ex-
tensive monitoring well data.
A number of reasons indicate that stressor-spe-
cific tolerance groups of plant species, constructed
with field data, are an effective basis for metrics in a
plant-based IBI:
• Linkages of plant species to specific stressors
are well documented
• Ecological suitability of a site for an individual
plant species is documented
• Field-based groups complement literature-
based ones
• Field-based construction improves the diagnos-
tic capabilities of metrics
An example of tabulation of field data for estab-
lishment of groups is presented in Figure D-1.
37
-------
Legend: 0% 26-50%
1-25% 51-75%
Sedimentation
Lowest — ^ Highest
Species Cluster 1 Cluster 2 Cluster 3 Cluster 4
n=16 n=12 n=5 n=l
Sediment Tolerators
Solidago patula
Solidago uliginosa
Typha latifolia
Senecio aureus
Dipsacus sylvestris
Fragaria virginiana
Moderately Tolerant
Solidago sp.
Carex vulpinoidea
grass
Impatiens capensis
Erythronium americanum
Carex prasina
Phalaris arundinacea
Slightly Tolerant
Carex sp,
Equisetum arvense
Arisaema triphyllum
Viola sp.
Thelypteris noveboracensis
Brachyelytrum erectum
Sediment Intolerant
Mentha arvensis
Onoclea sensibilis
Cirsium arvense
Eupatorium maculatum
Boehmeria cylindrica
Euthamia graminifolia
Glecoma hederacea
Potentilla sp,
Symplocarpus foetidus
Asclepias syriaca
Lysimachia nummularia
FIGURE D-l: SEDIMENT TOLERANCE GROUPS OF WETLAND PLANTS IN SLOPE
WETLANDS OF CENTRAL PENNSYLVANIA WETLANDS.
38
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