United States
                Environmental Protection
                Agency
                Region V
                Water Division
                230 South Dearborn Street
                Chicago, Illinois
EPA-905/3-84-002
February, 1984
                U.S. Department
                Of the Interior
                U.S. Fish and Wildlife Service
                Eastern Energy Land Use Team
                Route 3, Box 44
                Kearneysville, West Virginia 25430
c/EPA
The  Ecological  Impacts
Of Wastewater
On Wetlands
An Annotated Bibliography
  Do not WEED. This document
  should be retained in the EPA
  Region 5 Library Collection.

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                 EPA-905/3-84-002

            The Ecological Impacts of
              Wastewater on Wetlands
            an Annotated Bibliography
       U.S. Environmental Protection Agency
                     Region V
             Chicago, Illinois  60604
                       and
          U.S. Fish and Wildlife Service
         Eastern Energy and Land Use Team
       Kearneysville, West Virginia  25430
                  February 1984
Portions of this document were prepared under EPA
   Contracts No. 68-01-5989 and No. 68-04-5017
                  U S, Environmental Protection Agency
                  Region 5, library 
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                                       ACKNOWLEDGEMENTS
           Bibliographic work on wastewater and  wetlands  was begun by the Office
           of Biological Services  Eastern  Energy and Land Use Team  of  the U. S.
           Fish and Wildlife  Service and by Region V  of  the U.  S.  Environmental
           Protection Agency.  These  efforts  have been combined  to produce this
           annotated bibliography.

           Special thanks go to Kathleen Brennan, Steven Bach  and their associates
           at WAPORA, Inc. and to Lauren Lyons of the Eastern Energy and Land Use
           Team for their  vigorous bibliographic research.   ESEI,  Inc.  compiled
           the final document.  Jay  Benforado and  Catherine Grissom Garra served
           as Fish and Wildlife Service and Environmental Protection Agency project
           coordinators.
0

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                          TABLE OF CONTENTS
I.

II,

III,
                                                            Page

List of Tables	..	   ii

Introduction,,,,,,.,,,,,,,,,,,	..,,,,,,,,,,.......,,,,  iii

Study Guide.,	   ix

Bibliographic Entries,,	-	,,,,,	,.«,

A.  Entries (numbers 1 - 1065)
B.  Recent Additions (numbers  1066  - 1097)	,....
IV,
Index (following entry number 1097)	,,.  I
          The most recent additions to the bibliography
          are located at the end of Section III, follow-
          ing entry number 1065.  New citations are sep-
          arately alphabetized and numbered in sequence
          with the previous entries, beginning with num-
          ber 1066.  For the reader's convenience, these
          new entries have been inserted in proper al-
          phabetical order into the main entries by
          author and date.

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                             LIST OF TABLES
                                                                 Page
1,   Primary Sources of References for the
    Wastewater/Wetland Bibliography.......	.	  vi
2,  Computer Database Search Descriptors
    Used in Locating References ......................I.......... vii
3.  Key to the Two-Letter Code to Abstract Sources.	 viii
                                ii

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                               I.   INTRODUCTION

This annotated  bibliography  is a  compilation of references on the ecological
impacts of wastewater  addition to wetlands.   It is organized j.nto four sec-
tions*  The introduction  develops the background of wastewater application to
wetlands, provides  an  overview of the ecological issues involved and gives a
summary of methods, materials  and problems encountered with compiling the
references and  abstracts  for this publication.  A study guide section pro-
vides an overview discussion of wetlands and wastewater,  with key references
for those who are unfamiliar with these topics.  The third and largest sec-
tion of the document is an alphabetical and numerical listing of entries and
abstracts.  These are  arranged by the author's last name.  The final section
is an index to  subjects,  key words and geographical locations.

Background

The United States faces the  critical  task of improving the Nation's water
quality while containing  the costs of water pollution control.  Over the
years conventional  wastewater  treatment has  been based largely on expensive
and complicated "high  technology" practices.  Typically,  secondary treatment
standards are achieved by expensive and complicated processes such as acti-
vated sludge or trickling filters. This is  followed by a settling step and
the discharge of treated  effluent to  surface waters.  The solids produced by
wastewater treatment,  called sludge,  must be disposed of  by incineration,
land application, landfill or  ocean dumping.  Such conventional treatment
systems are well understood  and widely accepted by the sanitary engineering
community.  However, such operations  often are dependent  on large amounts of
energy, sophisticated  equipment,  chemicals and capital investments,  which
make them expensive to operate and maintain, particularly for smaller towns.
The wastewater  treatment  lagoons,  frequently used by small communities, can
provide reasonably priced secondary treatment, but this treatment level is
not always sufficient  to  meet  necessary effluent limitations.  In areas of
low stream flow, lake  discharge or other special demands  for water quality
improvement, the need  to  implement more advanced treatment (such as removal
of nutrients, additional  organics and suspended solids) compounds the
problems and costs of  wastewater  treatment.   Recent attention has been given
to the economic difficulties of achieving adequate treatment in existing
conventional facilities,  especially if advanced treatment levels are
required.  Sophisticated  conventional treatment methods may not succeed in
controlling  water pollution if the facilities are too difficult or expensive
for the owner or community to  maintain and operate at the treatment level for
which they were  designed.
                                       iii

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These economic pressures have intensified the  search  for  creative,  cost-
effective and environmentally sound ways to  control water pollution.   In this
context, more attention has been given  to developing  alternative approaches
to the treatment of wastewater.  The Clean Water Act  Amendments  of  1977 and
1981 have encouraged innovative and alternative treatment technologies,
especially for small communities.  One  technical approach is  to  use managed,
natural and artificially constructed ecosystems as a  functional  part of
wastewater treatment.  For example, land treatment of wastewater, which was
the only recognized technology before the advent of current sanitary engi-
neering solutions, is being reconsidered and further  developed as an effec-
tive form of wastewater treatment and pollution control.  Wastewater has been
successfully utilized as a water and nutrient  resource  in agriculture,  sil-
viculture, aquaculture, golf course and green  belt irrigation and other
areas.  Sludge has been used in forest  and agricultural crop  production to
reclaim and restore strip-mined lands and other heavily disturbed areas.  An
additional bonus to innovative and alternative technologies is the  approach
to wastewater as a resource rather than a liability.  Productive reuse and
recycling of the wastewater is realized from many of  these  technologies.

Using Wetlands

Although wetlands have traditionally been viewed as unproductive areas with-
out significant value, much information on the ecological functions and
natural values of these areas has been  documented during  the  last few
decades.  Wetlands serve as water reservoirs,  as links  between surface and
ground water, and as modulators and filters  in the hydrologic cycle.   They
are vital to fish and wildlife, and may serve  as critical habitat for rare
and endangered species.  Recreational,  aesthetic and  scientific  values are
also contributed by wetlands.  The role of wetlands in  global biochemical
cycling and atmospheric stability may be substantial.  A  special Federal
interest in protecting and enhancing wetlands  has been  recognized in
Executive Order 11990, "Protection of Wetlands", May, 1977  and in policies
developed by various Federal agencies.

Currently, the water purification ability of wetlands is  receiving  special
notice.  It is anticipated that this natural function can be  harnessed for
use in the renovation of wastewater.  The idea that wetlands  could  be useful
as a functional part of wastewater treatment has developed  partly as an
extension of the basic land treatment concept  and partly  as an effort to
improve the existing unit process approach to  wastewater  treatment.  Con-
structed units, termed "artificial" wetlands,  have been created  specifically
for wastewater treatment.  In some cases wetland plant  species have been
added to existing sewage lagoons while  at other sites new wetlands  have been
constructed.  At still other locations, natural wetlands  now function as an
                                       iv

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integral part  of  the  treatment facilities.   These efforts have shown that
wetlands can achieve  very  high levels  of treatment as well as provide other
values.  Some  small communities in areas with extensive wetland acreages and
few  large streams  for effluent discharge may be able to make use of. the
natural wetland resource to  renovate their  wastewater.  In other regions
where much of  the  original wetlands have been destroyed or degraded, there is
an opportunity to  use wastewater in wetland restoration.  This planned use of
wetlands for wastewater treatment contrasts with the unplanned and frequently
underdesigned  discharges of  wastewater to wetlands found at some locations.
In several parts  of the United States  where inventories have been conducted,
these unplanned discharges are more prevalent than newer, well designed
systems - often something  that "just happened" at a time when wetlands were
less well understood  and appreciated.

Ecological Concerns

Although wetlands  have been  recognized by natural scientists as offering some
potential for  treating wastewater,  work on  the engineering aspects has out-
paced an ecological understanding of the strengths, limitations and overall
aspects of wetland treatment.   This situation is compounded when system
planners are tempted  to take a "cookbook" approach to applying wastewater to
wetlands in the treatment  process,  not recognizing the variability among
different wetland  types and  site-specific biological and hydrological condi-
tions.  Some wastewater treatment applications have led to severe ecological
disruptions, while others  have had at  least short term success, without
evidence of ecological problems.   Little research on the ecological aspects
of wastewater  addition to  wetlands has been undertaken to date, especially on
long term effects.  This ecological understanding is essential to developing
a decision process to determine when a wetland alternative is workable and
when it is unwise.

This imbalance between engineering knowledge and ecological understanding
has created opposition, by some,  to the use of wetlands in the treatment of
wastewater.  Potential negative ecological  impacts resulting from the use of
wetlands must be fully considered.   Beneficial effects of wastewater treat-
ment and the potential enhancement of  wetlands and water bodies also merit
complete consideration.  Attempts  to assess ecological effects have been con-
fronted both by problems of  understanding natural processes in wetlands and
by the difficulty  of  locating  available ecological information on wetlands
and wastewater topics.

This annotated bibliography  is  an effort to help provide the information
necessary to develop  appropriate  wetland treatment techniques based on an
understanding of wetland ecology.   General  topics that are covered include:

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1) health concerns - human and wildlife, 2) effects  of  toxic  substances,  3)
adverse community changes, 4) effects on other wetland  functions  and  values,
5) long-term effects, 6) management potential, and 7) research  needs.


Bibliographic Research Methods


Entries in the bibliography were obtained  from a wide range of  sources/ pri-
marily conference and symposia proceedings, government  publications,  research
reports, and scientific journals.  The entry titles  were  collected  from those
sources, prepared bibliographies, and computer data  bases (Table  1).  A stan-
dardized list of key words used for all the data base searches  was  developed
(Table 2).  Approximately 55 percent of the entries  have  been annotated.  The
original author abstract was used whenever possible; otherwise  an abstract
was written from the report itself or an abstract from  one of the data bases
was used.  Each abstract is followed by two letters  that  identify its source.
A key to these letters is found at the beginning of  the list  of entries
(Table 3).
Table 1.  Primary sources of references for the wastewater/wetland  bibliography.


*DIALOG Information Retrieval Service
     Environmental Bibliography
     BIOSIS (Biological Abstracts)
     Aqualine
     Enviroline
     NTIS (National Technical Information Service)
     Pollution Abstracts
     IRL Life Sciences Collection

*Numerous conference and symposia proceedings, including St. Paul,  Minnesota
 1981 and 1983 and Amberst, Massachusetts, 1982.

*U.S. Environmental Protection Agency. 1983. The Effects of Wastewater Treatment
 Facilities on Wetlands in the Midwest.  Region V.  EPA 905/3-83-002.  Techni-
 cal Report.  Chicago.

*US Environmental Protection Agency, 1983.  Environmental Impact Statement.
 Phase I Report - Freshwater Wetlands for Wastewater Management.  EPA 904/9-
 83-107.  Region IV, Atlanta.

*Water Resources Abstracts

*Wetland Values Bibliography - U.S. Fish S Wildlife Service, Western Energy &
 Land Use Team.
                                       vi

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Two problems were  encountered  in locating relevant references.  The first was
difficulty in locating  applied materials  on wetlands.   Much of the needed in-
formation is in a  "gray area"  of literature which is difficult to assess.  No
single journal focuses  on  this topic  so one must search for items in both
biological and engineering journals/  government reports, wetland symposia and
other similar sources.   Such a diversity  of literature does not always show up
in computerized data bases because it lies between standard ecological and
engineering disciplines.   Computer data base searches  also pose problems because
the terms used to  describe wetlands are diverse and confusing.  Frequently these
are regional names  like pothole,  pocosin,  playa/lake,  vernal pool, slough, and
bottomland hardwood, to name a few.   Sometimes wetlands are included with an
aquatic system, like river, lake,  or  pond; at other times they are described as
littoral areas.  Artificial (constructed)  wetland terms also are plentiful -
lagoons, farm ponds, aquaculture,  hydroponics,  as well as others.
Table 2.  Computer database  search  descriptors used in locating references.
     wastewater                              wetland
     sewage                                  swamp
     water treatment                         marsh
     effluent                                bog
     nutrient removal                        lagoon
                                             aquaculture
                                             estuaries
                                             riverine
                                             riparian

Each of the descriptors in the  first  column  was paired with each descriptor in
the second column for one search  of the entries in each data base.
The second problem, of equal  difficulty,  was  limiting the scope of the bibliog-
raphy without sacrificing useful material.  Pew publications describe solely
ecological impacts of wastewater on  wetlands.   Considerable information is
available on peripheral subjects that  provide  insights into wetland/wastewater
issues.  These include a variety of  research  reports on aquaculture, an
overwhelming volume of material on pollution  in aquatic ecosystems (eutrophica-
tion, heavy metals, etc.), and considerable information on land treatment of
wastewater and sludge, as well as research on  conventional wastewater treatment
practices.  This selected bibliography will focus  on references that deal
directly with the use of wetlands, or  with the use of aquatic plants and
animals, in the treatment of  wastewater or the improvement of water quality.
Treatment of the foreign literature  is extremely limited in this document, an
area which would be worthwhile to pursue  in greater detail.
                                     vii

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Table 3.  Key to the two-letter code to abstract sources.
   Code Letters                                Source


 AA              Author's abstract or adapted from author's abstract

 AL              Aqualine, DIALOG Information Retrieval Service

 BA              BIOSIS, Biological Abstracts, DIALOG Information Retrieval
                 Service

 DM              Monge, D.  1978.  Bibliography of Wetland Values.  School of
                 Forestry.  State University of New York, Syracuse.

 EB              Environmental Bibliography, DIALOG Information Retrieval
                 Service

 EL              Enviroline

 EP              USEPA, 1983 (Region V).

 NT              National Technical Information Service, DIALOG Information
                 Retrieval Service

 PA              Pollution Abstracts, DIALOG Information Retrieval Service

 WE              Western Energy and Land Use Team, U.S. Fish and Wildlife
                 Service

 WR              Water Resources Abstracts
A SPECIAL NOTE:
As of this writing (Fall, 1983) EPA is drafting several generic Environmental
Impact Statements (EIS) on wastewater and wetlands.  Call or write directly to
learn more about these EIS's and to be included on their mailing lists.

Region V - Illinois,  Indiana, Michigan, Minnesota, Ohio, Wisconsin
           Catherine Grissom Garra
           US EPA Region V
           Water Division, Environmental Impact Section
           230 South Dearborn Street
           Chicago, IL  60604
           312-886-0241

Region IV - Alabama,  Florida, Georgia, Kentucky, Mississippi, North Carolina,
            South Carolina, Tennessee (freshwater or estuarine)
            Ronald Mikulak
            US EPA Region IV
            NEPA Compliance Section
            345 Courtland Street
            Atlanta,  GA  30365
            404-881-3776
                                      viii

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              STUDY GUIDE  FOR  WETLANDS  AND WASTEWATER TREATMENT

This section provides a guided entry  into the  subject matter of the Annotated
Bibliography.  The guide also  may  be  of value  to those who wish to review
current knowledge.  Key references introduced  under each subheading serve as
starting points  for further  research  and are not meant to be comprehensive.
The references at the end  of the Study  Guide that address general wetland in-
formation have been excluded from  the Annotated Bibliography.   All of the
references pertinent to the wetlands  and wastewater treatment  topic are
marked for the reader's convenience.

Wetland Ecosystems

Many types of wetland ecosystems exist  throughout the United States and any
understanding of their functions must begin with a knowledge of how these
wetlands are classified,   A variety of  classification schemes  has been devel-
oped since the 1950s, several  of which  are introduced here.  Shaw and Fredine
(1956) in Circular 39 identified 13 wetland types based primarily on the
needs and requirements of  waterfowl.  Regional classification  schemes have
been developed,  such as for  the Northeast (Golet and Larson, 1974) and the
Prairie Pothole Region of  the  Upper Midwest (Stewart and Kantrud, 1971),
More recently, the U.S. Fish and Wildlife (USFWS) Service developed a classi-
fication system, based upon ecological  characteristics of a  wetland,  that was
used in the National Wetland Inventory  (Cowardin et al,,  1979),

A primary concern in any wetland manipulation  is the nature  and extent of the
resultant system impacts.  Wetlands serve an important role  in the provision
of habitat, in nutrient cycling, and  in the flow regimes  of  both surface and
groundwater.  Increasing attention is being given to their value and
sensitivity.  Several books address general and/or specific  wetland ecosystem
impacts,  Greeson et al, (1979) provides a comprehensive  look  at the diverse
aspects of wetlands - scientific,  social political,  and economic.  Ecological
processes and management potential of freshwater wetlands are  discussed by
Good et al, (1978) and Richardson  (1981),   Weller (1981)  provides a perspec-
tive on the ecology and wildlife management potential in  freshwater marshes,
Kusler (1983) gives an overview of wetland values and outlines protection
strategies.

Wetland Treatment Capability

The concept of utilizing biological and ecological processes found in the
natural environment to treat wastewater is not new.   Applying  wastewater  and
sludge to the land has evolved from the routine agricultural practices of re-

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cycling manure and other agricultural residues,  Tourbier  and  Pierson (1976)
provide a good overview of these concepts and practices  as well  as  the U.S.
Environmental Protection Agency (USEPA) in several process design manuals (US
EPA, 1981; USEPA, 1983b).

With regard to its regulatory responsibilities,  the USEPA  has  been  pursuing  a
"technology based" approach to controlling water pollution and considers
natural treatment systems, such as wetlands, well suited to  meet their goals.
The concept has been shown to be viable and qualifies under  current USEPA
definitions as innovative or alternative technology.  Bastian  and Benforado
(1983) discuss the eligibility of this emerging  technology for Federal grant
assistance.

The capability of wetlands to renovate wastewater and to tolerate effluent
discharges has been demonstrated in a variety of natural and artificial
(constructed) wetlands across the nation.  Pull-scale aquatic  treatment sys-
tems, as well as numerous research and development projects, are operating or
under construction in over a dozen states and in several foreign countries.
These include natural and constructed wetlands,  treatment ponds,  systems
using floating aquatic plants grown in ponds or  ditches, and aquaculture
operations involving a variety of freshwater or  marine organisms.   The Uni-
versity of Michigan (northern peatlands and cattail marshes) and the Univer-
sity of Florida (cypress domes and strands) received funding during the 1970s
from the National Science Foundation  (NSF) to conduct long term  studies on
wetland treatment systems.  Many other universities are  involved in similar
research including, but not limited to, the Louisiana State  University
(southern hardwood swamps), Humboldt State University, California (artificial
freshwater wetlands), Rider College and Rutgers  University,  New  Jersey
(freshwater tidal marshes), the University of California - Davis (artificial
freshwater wetlands) the University of Wisconsin (northern peatlands and
cattail marshes), and Woods Hole Oceanographic Institute, Massachusetts,
(coastal brackish water wetlands).  Several publications provide lists of
currently operating wetland treatment systems (Chan et al.,  1982; Hammer et
al,, 1983; USEPA 1983d).

A number of types of natural wetlands have been  used to  investigate the
effectiveness and ecosystem impacts of wastewater application.  Northern
wooded swamps, cattail marshes, southern cypress swamps, freshwater tidal
marshes, salt marshes, and others have been manipulated.  Artificial or con-
structed wetlands have recently been used in the treatment of  wastewater.
Listings for all of these types of wetlands can  be found in  the  Index of this
document.  The use of artificial systems shows great promise for more general
application across the country.  They are more reliable  and  involve less risk

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of adverse environmental impact because  of  better  process control.   The use
of artificial water hyacinth systems was pioneered by Wolverton and others at
the NASA lab in Bay St, Louis, Mississippi  (Wolverton et al.,  1975).  Con-
structed wetlands also include aquaculture  systems that  both  treat  wastes and
produce valuable aquatic plants or animals,  and  marine polyculture  systems
which use wastewater and several organisms  in a  marine food chain
relationship (Bastian and Reed, 1979),

Bastian and Reed (1979; and Reed and Bastian, In Press)  provide an  engineer-
ing perspective on the capability of wetland systems to  renovate wastewater.
Other engineering assessments have been  written  by Chan  et al., (1982), Hyde
et al., (In Press), and Tchobanoglous and Gulp  (1980),   Under  the right
conditions wetland systems can achieve high removal  efficiencies for BOD,
suspended solids, trace organics and heavy  metals.   However,  specific factors
responsible for such high treatment levels  are not clearly understood.  The
relationship between wastewater nitrogen and phosphorus  application rates and
the efficiency of nitrogen and phosphorus removal  by wetlands  is described by
Nichols (1983).  An extensive list of references on  the  capacity of natural
wetlands to remove nutrients from wastewater is  included in this article.

Criteria for Design and Ecological Management

A major engineering limitation is that optimum,  cost-effective design
criteria for such wetland treatment systems  are  not  applicable across the
country.  Progress is being made in this area of research. Published data
have been  analyzed to identify general  principles for the successful design
and operation of wetland facilities (Hammer and  Kadlec,  1983;  Hammer et al.,
1983; Heliotis, 1981),  In both of the Hammer papers, variables in  the
wetland treatment process are manipulated in models  which may provide more
generally applicable design criteria.  The  Heliotis  thesis includes a
detailed literature review,

Richardson and Nichols (In Press) present ecological management criteria that
should be addressed prior to the selection  of a  wetland  treatment system.
These criteria include the value of the  effluent as  a resource, the capabil-
ities and limitations of wetlands to accomplish  wastewater treatment, waste-
water management objectives, wastewater  suitability  for  wetland discharge and
wetland values.  Benforado (1981) and Reed  and Kubiak (In Press) discuss
ecological considerations in the overall wetlands  manipulation process.

Several general papers provide broad perspectives  on a specific topic.
Tchobanoglous et al, (1979) has written  an  overview  on the use of aquatic
plants and animals in wetland wastewater treatment systems,   Kadlec and Til-
ton (1979) reviewed the use of freshwater wetlands as an alternative to ter-
tiary wastewater treatment.
                                      xi

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A number of symposia and/or conferences on wetlands and  their  role  in  waste-
water treatment have been held.  Three of the more recent and  important  con-
ferences on this subject are:  (1) The Midwest Conference on Wetland Values
and Management held June 17-19, 1981, in St, Paul, Minnesota;  (2) The  Fourth
Annual Meeting of the Society of Wetland Scientists held June  5-8,  1983  in
St. Paul, Minnesota; and (3) Ecological Considerations in Wetlands  Treatment
of Municipal Wastewaters, a Workshop at the University of Massachusetts
held June 23-25, 1982, in Amberst, Massachusetts.  Papers presented at these
meetings or published proceedings have provided major contributions to the
understanding of wetland treatment systems and their role in wastewater
management.

A number of Federal agencies are interested and have been involved  to  various
degrees in funding or coordinating research on the use of wetlands  for waste-
water treatment.  These agencies include, among others,  the National Aero-
nautic and Space Administration (NASA), NSF, USEPA and USFWS.  The  latter two
agencies co-sponsored the 1982 Antherst Workshop.  Some of the  notable  USEPA
funded research has come from the Municipal Environmental Research  Lab (MERL)
in Cincinnati, Ohio and the Robert S. Kerr Research Lab  in Ada, Oklahoma.
The Ada Research Lab has funded projects in Santee, California (San Diego
Regional Water Reclamation Agency), at the University of Wisconsin, Milwau-
kee, and in Bay St. Louis, Mississippi, as well as others.  The work cited
earlier by Hammer and Kadlec (1983) was sponsored by the Robert S.  Kerr  Lab,

USEPA is currently conducting studies that address wetlands and wastewater on
a regional basis (Regions V and IV),   A Region V interim document  describes
the effects of wastewater treatment facilities on wetlands in  the six  mid-
western states (USEPA, 1983c).  This work provides the background information
needed in preparation for a Generic Environmental Impact Statement  (EIS) on
guidelines for wetland treatment systems.  A Region IV EIS assesses the  use
of freshwater wetlands for wastewater disposal in the southeastern  states
(USEPA, 1983a).  The second phase of the report, when published, will  develop
a handbook to delineate methods of analysis as well as safeguards and  guide-
lines for evaluating, selecting, permitting, and monitoring wetland disposal
systems.  Both USEPA documents provide good overviews and have extensive
bibliographies,

Institutional Considerations

Institutional constraints can severely limit the ability of regulatory agen-
cies to implement otherwise sound concepts and technology.  Critical institu-
tional considerations concerning the use of wetlands for wastewater treatment
include a) regulatory policies concerning conflicts between wetland protec-
tion and use, b) ownership and proprietary rights of private wetlands, and
                                   xii

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c) various agency regulations and policies associated with  permitting
processes.  While no comprehensive studies have addressed this  vital  aspect
of wetlands and wastewater, insights may be obtained from Kusler  (1980,  1983)
Rusincovitch (In Press) and publications of the National Wetland  Newsletter,
Environmental Law Institute, Washington, D.C.   Reed and Kubiak (In press)
present a formal ecological evaluation process to determine wetland suitabil-
ity for wastewater treatment and discharge.  They further discuss how formal-
ized wetland criteria can be adopted into the institutional framework of the
facilities planning process.  In addition, both USEPA regional  studies will
explore this topic.
                                     Kill

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                                    REFERENCES

*  1.  Bastian, R.K. and J. Benforado.  1983.  Waste Treatment:  Doing What
           Comes Naturally.  Technology Review 86(2):59-66.  Feb. - Mar.

*  2.  Bastian, R.K. and S.C. Reed. (ed).  1979.  Aquaculture Systems for
           Wastewater Treatment:  Seminar Proceedings and Engineering Assess-
           ment.  EPA 430/9-80-006.  U.S. Environmental Protection Agency,
           Office of Water Program Operations, Municipal Construction Divi-
           sion.  Washington, D.C.  485 p.

*  3.  Benforado, J. 1981.  Ecological Considerations in Wetland Treatment of
           Muncipal Wastewater.  In  Selected Proceedings of the Midwest
           Conference on Wetland Values and Management.  June 17-19.  St. Paul,
           MN.  pp. 307-323.  Minnesota Water Planning Board; Water Resources
           Research Center, University of Minnesota; Upper Mississippi River
           Basin Commission and the Great Lakes Basin Commission.

*  4.  Chan, E., T.A. Bursztynsky, N. Hantzsche and Y.J. Litwin, 1982.  The
           Use of Wetlands for Water Pollution Control.  USEPA Office of
           Research and Development, Municipal Environmental Research Lab.
           Cincinnati.  600/S2-82-086.  November.

   5.  Cowardin, L.M., V. Carter, F.C. Golet, and E.T. LaRae.  1979.  Classifi-
           cation of Wetlands and Deepwater Habitats of the United States.  U.S.
           Fish and Wildlife Service.  FWS/OBS-79/31.

   6.  Golet, F.C. and J.S. Larson.  1974.  Classification of Freshwater Wet-
           lands in the Glaciated Northeast. U.S. Fish and Wildlife Service.
           Res. Pub. 116. 56 p.

*  7.  Good, R.E., D.F. Whigham and R.L. Simpson (ed). 1978.  Freshwater Wet-
           lands: Ecological Processes and Management Potential.  Academic
           Press, Inc., New York.

0  8.  Greeson, P.E., J.R. Clark, and J.E. Clark (ed).  1979. Wetland Functions
           and Values:  The State of Our Understanding.  Proceedings of the
           National Symposium on Wetlands.  Lake Buena Vista, Florida.  Amer.
           Water Resour.  Tech. Pub. TPS 79-2.  Minneapolis, MN.

*  9.  Hammer, D.E. and R.H. Kadlec.  1983. Design Principles for Wetland Treat-
           ment Systems.   Project Summary.  EPA-600/S2-83-026.  Robert S. Kerr
           Environmental Research Laboratory.  Ada, OK. May. (See also Hammer,
           D.E. et al., 1983 for complete version of this article.)

* 10.  Hammer, D.E. et al. 1983.  Design Principles for Wetland Treatment
           Systems.  University of Michigan - Ann Arbor.  National Technical
           Information Service No. PB 83-188722.  April.  (See also Hammer, D.E.
           and R.H. Kadlec, 1983 for USEPA Project Summary version of this arti-
           cle. )

* 11.  Heliotis, F.D. 1981.  Wetland Systems for Wastewater Treatment: Operating
           Mechanisms and Implications for Design.   University of Wisconsin -
           Madison.  Master's Thesis.  103 p.
                                        xiv

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0 12.  Hyde, H.C., R.S. Ross and F. Demgen.  In press.  Technical Assessment of
           Wetlands for Municipal Wastewater Treatment.  USEPA Office of
           Research and Development, Municipal Environmental Research Lab.
           Cincinnati.

* 13.  Kadlec, R.H. and D.L. Tilton.  1979.  The Use of Freshwater Wetlands as
           a Tertiary Wastewater Treatment Alternative.  CRC Critical Reviews
           in Environmental Control 9(2}: 185-212.

0 14.  Kusler, J.A. 1980.  Regulating Sensitive Lands.  Ballinger Pub. Co.
           Cambridge, MA.  248 p.

0 15.  Kusler, J.A. 1983.  Our National Wetland Heritage.  A Protection Guide-
           book.  Environmental Law Institute,  Washington, D.C.  168 p.
* 16.  Nichols, D.S. 1983.  Capacity of Natural Wetlands to Remove Nutrients
           from Wastewater.  J. of Water Pollut. Cont.  Fed. 55(5) .-495-505.

* 17.  Reed, S.C.  and R.K. Bastian. In press. Wetlands for Wastewater Treatment
           An Engineering Perspective.  In  Ecological Considerations in Wet-
           lands Treatment of Municipal Wastewaters.  Proceedings of a Workshop
           June 23-25, 1982.  University of Massachusetts.  Amherst, MA.  U.S.
           Fish and Wildlife Service and U.S. Environmental Protection Agency.

0 18.  Reed, C.A.v and T.  Kubiak.  In press.  An Ecological Evaluation Procedure
           for Determining Wetland Suitability for Wastewater Treatment and Dis-
           charges.  In  Ecological Considerations in Wetlands Treatment of
           Municipal Wastewaters, Proceedings of a Workshop, June 23-25, 1982.
           University of  Massachusetts, Amherst, MA. US Fish and Wildlife Ser-
           vice and US Environmental Protection Agency.

0 19.  Richardson,  P. (ed).  1981.  Selected Proceedings of the Midwest Con-
           ference on Wetland Values and Management.  June 17-19.  St. Paul,
           Minnesota.  Minnesota Water Planning Board;  Water Resources Research
           Center,  University of Minnesota; Upper Mississippi River Basin Com-
           mission and Great Lakes Basin Commission.
  20.   Richardson,  C.J.  and D.S.  Nichols. In press.  Ecological Analysis of Waste-
           water Management Criteria in Wetland Ecosystems.   In  Ecological
           Considerations in Wetlands Treatment of Municipal Wastewaters.   Pro-
           ceedings of a Workshop June 23-25, 1982.  University of Massachusetts.
           Amherst, MA.   U.S. Fish and Wildlife Service and U.S.  Environmental
           Protection Agency.

  21.   Rusincovitch, F.  In press. Use of Wetlands for Wastewater  Treatment and
           Effluent Disposal: Institutional Constraints.   In  Ecological Consid-
           erations in Wetlands Treatment of Muncipal Wastewaters.  Proceedings
           of a Workshop June 23-25, 1982.   University of Massachusetts.
           Amherst, MA.   U.S. Fish and Wildlife Service and U.S.  Environmental
           Protection Agency.

  22.   Shaw,  S.P.  and C.G. Fredine.   1956.   Wetlands of the United States.  Cir-
           cular 39.  U.S. Fish and Wildlife Service.
                                       xv

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  23.  Stewart, R.E.  and H.A.  Kantrud.   1971.  Classification of Natural Ponds
           and Lakes  in the Glaciated Prairie Region.  U.S.  Bureau of Sport Fish-
           eries and  Wildlife  Resources.   Pub. 92.  57p.

* 24.  Tchobanoglous, G.  and G.L.  Gulp. 1980.   Wetland Systems for Wastewater
           Treatment: An Engineering Assessment. In  Aquaculture Systems for
           Wastewater Treatment:   An Engineering Assessment.  EPA 430/9-80-007.
           June.

* 25.  Tchobanoglous, G., R. Stowell, R.  Ludwig, J. Colt, and A. Knight.  1979.
           The Use of Aquatic  Plants and Animals for the Treatment of Waste-
           water:  An Overview,  pp. 35-55.  In  R.K. Bastian and S.C. Reed (ed).
           Aquaculture Systems for Wastewater Treatment:  Seminar Proceedings and
           Engineering Assessment.  EPA 430/9-80-006.   U.S. Environmental Pro-
           tection Agency, Office  of Water Programs, Municipal Construction
           Division.   Washington,  D.C.

* 26.  Tourbier, J. and R.W. Pierson (ed.).  1976.   Biological Control of Water
           Pollution.  Univ. of Pennsylvania Press.  Philadelphia.

  27.  U.S. Environmental Protection Agency. 1981.   Process Design Manual for
           Land Treatment of Municipal Wastewater.   EPA 625/1-81-013.

* 28.  U.S. Environmental Protection Agency. 1983a.  Freshwater Wetlands for
           Wastewater Management.   Environmental Impact Statement.  Phase I
           Report.  EPA 904/9-83-107.  Region IV, Atlanta.

  29.  U.S. Environmental Protection Agency. 1983b.  Process Design Manual for
           Land Application of Municipal Sludge.  EPA 625/1-83-016.  October.

* 30.  U.S. Environmental Protection Agency. 1983c.  The Effects of Wastewater
           Treatment  Facilities on Wetlands in the Midwest.  Technical Report.
           Region V.   EPA-905/3-83-072.  September.

* 31.  U.S. Environmental Protection Agency. 1983d. Wetlands Treatment:  A Prac-
           tical Approach.  (Brochure)  EPA Emerging Technology Series.

  32.  weller, M.W. 1981.  Freshwater Marshes:  Ecology and Wildlife Management.
           University of Minnesota Press, Minneapolis, MN.   146 p.

* 33.  Wolverton, B.C., R.M. Barlow and R.C. McDonald.  1975. Application of
           Vascular Aquatic Plants for Pollution Removal, Energy and Food Pro-
           duction in a Biological System.  NASA.  NSTL.  Bay St. Louis, MS.
  0  Key references on wetlands/wastewater found in Bibliography.

  *  Key references on wetlands/wastewater found in Bibliography
     with annotation.
                                      xvi

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1. Abel, P. D.  1974.  Toxicity of Synthetic Detergents  to Fish  and Aquatic
      Invertebrates.  J. Fish. Biol. 6(3):279-298.

      Synthetic detergents are reported to be acutely toxic  to fish in  con-
   centrations between 0.4 and 40 mg/1.  Factors affecting toxicity include
   the molecular structure of the detergent, water hardness,  temperature  and
   dissolved oxygen concentration; the age and species of the test  fish,  and
   acclimation to low concentrations of detergent.  Some of  these  factors
   appear to be of only limited importance.  Gill damage is  the  most obvious
   acute toxic effect;  the immediate cause of death may be  asphyxiation, but
   detergents may also be toxic internally.  Lethal effects  not  related to
   gill damage have not been investigated.  Sublethal effects include
   retardation of growth, alteration of feeding behaviour and inhibition  of
   chemoreceptor organs.  Low levels of detergents may also  increase the
   uptake of other pollutants. Invertebrates, especially in  their  juvenile
   stages, are extremely sensitive to detergents: concentrations below  0.1
   mg/1 interfere with growth and development in some species.   The inter-
   actions between detergents and proteins, and their influence  on  membrane
   permeability may be the basis of the biological action of  detergents.
   Detergents in natural waters are usually partially degraded,  and a maximum
   permissible concentration of 0.5 mg/1 would probably  be harmless under
   most conditions.  (AA)
2. Abeliovich, A. 1980.  Factors Limiting Algal Growth  in High-rate Oxida-
      tion Ponds.  In;  Proc. Int. Symp. Prod, and Use  of Alga Biomass.
      Shelef and Boeder (Eds.) Elsevier, Amsterdam.
3. Adriano, D. C., L. T. Novak, A. E. Erickson, A. R. Wolcott, and B. G.
      Ellis.  1975.  Effect of Long Term Land Disposal by Spray  Irrigation
      of Food Processing Wastes on Some Chemical Properties of the Soil and
      Subsurface Waters.  J. Environ. Qual. 4:244-248.

      Food processing waste waters at two irrigated land disposal  sites and
   subsurface waters (perched and ground waters) were monitored  at daily  to
   monthly intervals over one annual cycle of production.  Soil  profiles
   were sampled to depths up to 6.6m in the early fall.
      Yearly inputs were calculated at 487 kg/ha total N (Kjeldahl plus NO3-N)
   and 101 kg/ha soluble PO4~P (orthophosphate) from cannery wastes  at site
   1.   Estimates for milk wastes at site 2 were 562 kg/ha total  N  and   522
   kg/ha PO4-P.
      The range for NC^-N in subsurface waters was 7 to 16 ppm at  site 1
   (perched water at 1,5m) and 2 to 41 ppm at site 2 (ground water at 0.9 m).
   Maximum concentrations, found in summer, were essentially the same as  the
   average for total N in the input wastes (16 ppm at site 1 and 38  ppm at
   site 2).  Nitrate was stable in the percolation stream below  the  root
   zone.  Annual additions to subsurface waters were estimated at  76% of  in-
   put N at site 1 and 65% at site 2.
      The range of PO^-P in subsurface waters was 0.5 to 1.5 ppm at  site  1
   and 0.04 to 1.8 ppm at site 2; average waste water concentrations were 3

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   and 35 ppm.  The highest concentrations in subsurface water were found  in
   spring.   Annual subsurface discharge was estimated at 27% of input P at
   site 1 and 2% at site 2.  The extensive removals of PC>4 and the similar
   concentrations encountered in subsurface waters are of theoretical and
   practical interest since P04~P had already accumulated in soil profiles
   at both sites in quantities which exceed the Langmuir maxima for nonirri-
   gated control soils.
      During seasons of major irrigation input, HO^ appeared in subsurface
   waters in concentrations exceeding public health standards; P04 concen-
   trations exceeded environmental guidelines at all times except where irri-
   gation was discontinued during the winter at site 2.
      Soil systems appeared poised to discharge at the observed rates because
   of the large quantities of organic N and fixed P which had accumulated  in
   the profiles over 20 years operation at site 1 and 10 years at site 2.  The
   rate of residual accumulation in soil could have been reduced by harvest
   to extend system life materially.  The harvest potential of three grass
   clippings per season removed for silage was estimated experimentally at
   31% of input N at both sites, 80% of input PC>4 at site 1 and 27% at site
   2.  (AA)
4. Akin, E. W., et al.  1971.  Enteric Viruses in Ground and Surface Waters:
      a Review of their Occurrence and Survival, pp. 59-74.  In V. Snoeyink
      and V. Griffin  (eds.). Virus and Water Quality: Occurrence and Con-
      trol.  Univ. of Illinois, Urbana-Champaign.
5. Alabaster, J. S.  1959.  Effect of Sewage Effluent on Distribution  of
      Dissolved Oxygen and Pish in a Stream.  J. Anim. Ecol., 28:283-   .
6. Albrecht, S. C., and B. J. Barfield.  1981.  Use of a Vegetative Filter
      Zone to Control Fine-Grained Sediments from Surface Mines.  EPA
      600/57-81-117.  U.S. Environmental Protection Agency.  Industrial
      Environmental Research Laboratory.  Cincinnati, OH.

      The objective of this study was "to demonstrate the effectiveness  of  a
   vegetative filter zone in trapping fine-grained sediments from surface
   mining operations."  The area selected for study was located in Whitley
   County, Kentucky, directly below an active surface mining operation.  The
   outslope above the filter was the primary drainage area monitored during
   this study.
      Results of the monitoring efforts revealed that a dramatic reduction  in
   sediment load was achieved by vegetative filtration for particle sizes
   larger than clay.  Based on the results of this study, it was concluded
   that vegetatative filters are an effective control for reducing the
   quantity of sediment transported into surface streams and rivers from
   disturbed mined lands.

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 7. Alleman, J. E., and S. P. Lo.  1980.  Investigation of Full-Scale Bio-
       logical Batch Treatment of Intermittent Wastewater Flows.  Tech.  Rept.
       No 63.  Water Resources Research Center.  Univ. pf Maryland, College
       Park.  30 pp.
 8. Allen/ G., 6. Conversano, and B. Colwell.  1972.  A Pilot Fish-Pond
       System for Utilization of Sewage Effluents/ Humboldt Bay, Northern
       California.  California State Univ., Humboldt, Arcata.  Marine
       Advisory Extension Service.  31 pp.

       The paper documents all out-of-pocket and other real costs in the
    construction of a pilot fish pond system for the utilization of sewage
    effluents, Humboldt Bay, Northern California.  Discussions deal with the
    engineering and construction problems encountered.  A description of the
    project site and reasons for site selection are explained.  (NT)
 9. Allen, 6. H.  1969.  A Preliminary Bibliography on the Utilization of
       Sewage in Fish Culture.  FAO Fisheries Circ. #308.  Rome*
10. Allen, 6. H.  1972.  The Constructive Use of Sewage, with Particular
       Reference to Fish Culture.  Marine Pollution and Sea Life.  506-513.

       Giving a bibliography of 132 references, the author reviews methods
    for using sewage in aquaculture, particularly in fish culture, as in
    Germany, U.S.A., Israel, and India, and discusses the possibility of
    future world-wide developments, including the desirability of inter-
    national co-operation for research on the use of waste waters in inte-
    grated agriculture aquaculture systems.  (AS)
11. Allen, G. H.  1976.  Rearing Pacific Salmon in Saltwater Ponds Fertilized
       with Domestic Wastewater.  Sept. 1974-Nov. 1975 Data Kept. HSU-SG-10,
       Humboldt State Univ., Arcata, CA.  92 pp.
12. Allen, G. H., and R. A. Gearheart.  1978.  Arcata Integrated Wastewater
       Treatment, Reclamation, and Salmon Ranching Project.  California State
       Univ., Humboldt, Arcata.  Marine Advisory Extension Service.  NOAA,
       Office of Sea Grant.  76 pp.
13. Allen, G. H., and B. Hepher.  1976.  Recycling of Wastes Through Aqua-
       culture and Constraints to Wider Application.  FAO Tech. Conf. on
       Aquaculture.  Kyoto, Japan.

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       Pacific salmon smolts have been reared in saline ponds fertilized with
    domestic wastewaters.   An artificial homestream is to be  created from the
    discharge of a marsh-lake system to be developed with reclaimed waste-
    water.   This proposed  salmon ranching method has the potential for
    producing food cheaply.  (NT)
14. Allen, J.  and S.  S. Wilson.   1977.  A Bibliography of References to Avian
       Botulism.   U.S.  Fish and  Wildlife Service Special Scientific Report -
       Wildlife No.  24.  Department of the Interior, Washington, DC.  6 pp.
15. Allen, M.  B.   1955.  General Features of Algal Growth in Sewage Oxidation
       Ponds.   Calif.  State Water Poll.  Control Bd. Publ. #13.
16. Allison, J.,  and J.  L.  Fox.   1976.  Coliform Monitoring of Waters Associ-
       ated with  the Cypress Dome Project, pp.  279-290.   In H. T. Odum, K. C.
       Ewel, J. W.  Ordway,  and M. K.  Johnston (eds.), Cypress Wetlands for
       Water Management, Recycling, and Conservation.  Third Annual Report.
       Center for Wetlands.  Univ. of Florida.   Gainesville.
17. Althaus, H.  1966.  Biological Wastewater Treatment with Bulrushes.
       G.W.F. 107(18):486-488.
18. American Water Works Association Research Foundation.  1979a.  Proceed-
       ings of the Water Reuse Symposium.  Volume 1.  Held 25-30 March in
       Washington, DC.  Co-sponsored with U.S. Department of the Interior,
       Office of Water Research and Technology;  U.S. Environmental Protection
       Agency; National Science Foundation; and Water Pollution Control
       Federation.  Denver, CO.  782 pp.
19. American Water Works Association Research Foundation.  1979b.  Proceed-
       ings of the Water Reuse Symposium.  Volume 2.  Held 25-30 March in
       Washington, DC.  Co-sponsored with U.S. Department of the Interior,
       Office of Water Research and Technology; U.S. Environmental Protection
       Agency; National Science Foundation; and Water Pollution Control
       Federation.  Denver, CO.  332 pp.
20. American Water Works Association Research Foundation.  1979c.  Proceed-
       ings of the Water Reuse Symposium.  Volume 3.  Held 25-30 March in
       Washington, DC.  Co-sponsored with U.S. Department of the Interior,
       Office of Water Research and Technology; U.S. Environmental Protection
       Agency; National Science Foundation; and Water Pollution Control Fed-
       eration.  Denver, CO.  1626 pp.

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21. Anderson, R. K., and D. Kent.   1979.  Progress  Report:   Drummond,
       Wisconsin, Tertiary Treatment Demonstration  Project  Study.   Report to
       the U.S. Fish and Wildlife Service.  University  of Wisconsin,  Stevens
       Point, WI.   22 pp.

       The Drummond, Wisconsin wastewater treatment facility consists  of  a
    primary and two secondary reservoirs.  Secondary  effluent is  spread on
    the surface of  a nearby bog by  means of a  gated irrigation pipe.   Theo-
    retically, the  receiving bog will provide  tertiary  treatment  to the
    effluent without deleterious effects to the  ecosystem.   The initial dis-
    charge of effluent occurred on  June 15, 1979.
       The study area is a 25-acre  sphagnum bog  in  sections 28 and 33,
    T45N, R7W, Bayfield County, Wisconsin, which  drains into a 4.1-acre
    bog lake (Weso Lake) and thence to the Long Lake  Branch of the White
    River.  The objective of this study is to  determine the effects
    of the secondary effluent on the fauna of  the receiving bog.
22. Anon.  1970.  Studies of Marine Estuarine Ecosystems  Developing with
       Treated Sewage Wastes. University  of North Carolina,  Institute of
       Marine Sciences Annual Report for  1969-1970.  NTIS No. PB-199 537.
       May.  364 p.

       This is the second annual report in a study  of  ecological  systems
    that develop when treated wastes from municipal  sewage systems  that
    develop when treated wastes from municipal sewage  systems flow  into
    estuarine waters.  Studies by a team  of faculty  and students  consid-
    ered a small marsh-lined estuary, Calico Creek,  which received  wastes
    following secondary treatment, and a  set of three  ponds  in which est-
    uarine water and treated sewage mixture flows.   Three control ponds
    received tapwater and estuarine water.  In their second  year, the ponds
    were rich in productivity with successive algal  blooms throughout the
    year and a food chain culminating in  blue crabs.   Chapters are  included
    on the events in the salinity regime  and input  management.  Photosyn-
    thetic productivity and respiration,  algal growth, phosphorous  and nitro-
    gen, bacteria, and animal populations.  The presence  of  a substantial
    ecological system suggests a viable intermediary system  interface is
    possible between man's municipal wastes and normal estuaries.   These
    systems have potential for aquiculture and waste amelioration.
23. Anon.  1972.  Outdoor Mass Culture of Marine Phyto-plankton.   Aquacul-
       ture 1(2):181.
24. Anon. 1975a.  Floating Aquatic Plants Remove Chemicals  from Polluted
       Waters.  Water and Pollution Control, 113(6): 23-25.

       NASA's National Space Technology Laboratories (NSTL) in Bay  St. Louis,
    Mississippi has been experimenting with water hyacinths to determine the
    plant's ability to absorb and concentrate toxic metals, and metabolize
    other chemical pollutants - phenols, creosols, insecticides, nitrates, and

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    phosphates.  In early 1975 large quantities of water hyacinths were
    planted in a portion of the 60-acre lagoon that serves as the city's  total
    sewage disposal outlet.  The planting begins a complex process that
    results in the total recycling of enormous quantities of water hyacinths.
    One month after initial stocking, plant harvesting begins; by this time
    the plants will have reproduced enough to appreciably reduce pollution
    levels.  The harvested crop is subjected to procedures to determine  im-
    purity assimilation.  Two methods under study by NSTL involve continual
    processing of the water hyacinths.  Anaerobic fermentation converts
    shredded water hyacinths into bio-gas, similar to natural gas.  The poten-
    tial yield is expected to be 28,500 cubic meters of gas per acre  of plants.
    Pyrolytic decomposition produces a mixed hydrocarbon fuel similar to  bio-
    gas.  Plants grown in domestic sewage effluent (low in toxic metal con-
    tent) will be evaluated as an animal food source.  Residues from  these
    processes will yield high grade fertilizer.  The advantages of the inte-
    grated aquatic plant disposal method are durability and low installation
    and maintenance costs.  An obvious drawback is that it is limited to
    tropical and subtropical climate zones.
25. Anon. 1975b.  Marshes and Wetlands May Provide Safe Community  Tertiary
       Sewage Treatment.  Compost Science, 16(4):31.

       The preliminary conclusion of a University of Michigan Research Project
    now in its third year is that marshes, bogs, swamps and  other  wetlands are
    able to provide high-quality tertiary sewage treatment.   The project was
    designed to find out whether the natural processes in  a  1,000  acre marsh
    near Houghton Lake in North Central Lower Michigan will  be  able  to provide
    tertiary balance.  The research has included a survey  of  pertinent scien-
    tific literature, the construction of a computer model of the  marsh, and
    small, carefully controlled, on-site experiments.  The model portrays the
    dynamic character of the marsh and accurately simulates  the complex inter-
    actions among soil, water, wildlife, and climate.  The model predicts that
    when millions of gallons of secondarily treated effluent are pumped into
    the marsh, the four-foot layer of peatland  covering the  area will  absorb
    many of the dissolved nutrients in the water; only a small  portion of the
    total will be utilized by vegetation.  Wetlands bordering lakes  and
    streams must not be used for treatment because of the  risk  that  effluent
    would reach adjacent waters before being  sufficiently  purified.  Each
    potential treatment site must be thoroughly studied to ensure  that the
    wetland will be able to remain in an aesthetic and viable state, and the
    water quality will meet environmental standards.
26. Anon. 1977a.  Concept, Research, Approval...An Effluent Irrigation Project
       Houghton Lake :vwer Authority.  Consulting Engineer.  48(5):77.

       A marshland irrigation system was proposed for  the  disposal of treat-
    ment plant effluents  in Michigan.  Two possible  advantages  were seen:   an
    estimated cost savings of $700,000 over  the use  of a conventional irriga-
    gation system, and improved food supply  and wildlife proliferation in the
    marsh area.  Because  of the lack of data on wetlands irrigation in Michi-
    gan, a test program was conducted before project approval.   The test area

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    was evaluated through plant, soil, and insect samples and through water
    quality tests.  The organic deposits proved excellent in nutrient removal
    without overloading, and no adverse effects were detected in  the test pro-
    gram.   The application of 12.5 million gallons of wastewater  during 1975
    and 1976 continued to produce favorable results without adverse effects.
    The marsh ecosystem was not damaged, and plants grew larger and greener.
    The irrigation project was accepted as financially and environmentally
    feasible.
27. Anon. 1977b.  Lab to Test Bulrushes as Filters for Sewage.  Water  and
       Wastes Engineering  14(9):16.  September 1977.

       Environmental Quality Laboratory, Inc., a subsidiary  of General Devel-
    opment Corporation, is investigating the use of bulrushes to  create  a
    low-cost, natural means of tertiary sewage treatment.  In experiments  at
    Port Charlotte, Florida, 6 inch bulrush plants were planted in plastic-
    lined troughs which were filled with water to create a half-acre artifi-
    cial marsh.  A 12-month testing program is being used to evaluate  phos-
    phorus and nitrogen removal from primary and secondary treated effluents
    by bulrushes in the waste water flooded artificial marsh.  Since ad-
    vanced waste water treatment is required for the future, the  marshland
    filtration system may provide tertiary treatment at lower construction
    and maintenance costs than conventional systems.
28. Anon. 1977c.  Using Wetlands for Wastewater Treatment.  Journal Water
       Pollution Control Federation.  49(7):1581.  July,  1977.

       The possible use of wetlands in Michigan and Florida for  final  treat-
    ment of municipal wastewater has been considered.  At a trial  site in
    Florida, wastewater is pumped into cypress ponds where nutrients are
    absorbed by vegetation.  Costs for this method of  treatment  are approxi-
    mately 50 percent less than for treatment by a conventional  plant.  At  a
    trial site in Michigan, wastewater is pumped into  a peat marsh for the
    removal of nutrients.
29.  Anon.  1979.  Treatment of Stormwater Runoff by a Marsh/Flood Basin.
       Association of Bay Area Governments.  Berkeley, CA.

       The flood basin functions as a marsh/wetland.  The  first  year's
    results of the study show that pickleweed dominates the  lower  elevation
    marsh areas that are subject to daily and seasonal inundation.   Pickle-
    weed also tends to concentrate heavy metals to a greater extent than the
    mixed marsh or upland grass vegetation.  The aerial plant parts of
    pickleweed demonstrates the ability to concentrate heavy metals -
    particularly Zn and Cd - at significant  levels beyond  soil and stormwater
    concentrations.

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30. Anon.  1980.  Solar Aquaculture Wastewater Treatment Plant.  Public Works
       (USA) 111(5):122.

       The Solar Aquacell System of wastewater treatment which utilizes natur-
    al ecological processes in an anaerobic pond lined and covered with a
    rubber membrane, creating a sealed chamber.  Here between 30-50 percent
    of solids are settled and digested.  Secondary treatment is in an aerated
    pond from which waste water flows to an aerated lagoon covered with water
    hyacinths and duckweeds which metabolize wastewater nutrients.  These
    ponds are enclosed in a solar-heated greenhouse.  Effluent from the
    lagoons is passed through a sand filter into an ozone contact chamber for
    final disinfection.
31. Anthony, R. G., G. R. Bierei, and R. Kozlowski.  1978.  Effects of
       Municipal Wastewater Irrigation on Select Species of Mammals, pp.
       281-287.  In H. L. McKim  (coor.), State of Knowledge in Land Treatment
       of Wastewater.  Vol. 2.  Proceedings of an international symposium
       held 20-25 August 1978 at Hanover, NH, sponsored by U.S. Army Corps of
       Engineers.
32. Anthony, R. G., and G. W. Wood.  1979.  Effects of Municipal Wastewater
       Irrigation on Wildlife and Wildlife Habitat, pp. 213-223.  In W. E.
       Sopper and S. N. Kerr (eds.), Utilization of Municipal Sewage Effluent
       and Sludge on Forest and Disturbed Land.  The Pennsylvania State
       University Press, University Park, PA.

       The effects of wastewater renovation on wildlife and wildlife habitat
    have been investigated in mixed oak and aspen/pine/shrub habitats.  Influ-
    ence of wastewater on vegetation and wildlife is dependent on the type of
    habitat irrigated and the species of wildlife in question.  Studies to
    date indicate no major detrimental effect of irrigation and wildlife and
    wildlife habitat; however, some influences are apparent.  The establish-
    ment of lush herbaceous undergrowth in mixed oak habitats decreases forage
    production for white-tailed deer, results in higher populations of white-
    footed mice during the fall, and appears to reduce stability and abundance
    of songbird populations.  The influences of wastewater irrigation in
    aspen/pine/shurb habitats on wildlife and wildlife habitat appear to be
    less negative.  In these habitats the quantity and quality of forage pro-
    duction for white-tailed deer and cottontail rabbits may be increased.
       Songbirds avoided irrigated sites while irrigation was in progress, but
    use of these areas was normal when irrigation was not in progress.  Song-
    birds also used irrigated mixed oak habitats less during early summer but
    significantly more during late summer.  Wastewater irrigation appeared to
    favor species of songbirds at the expense of diversity.
       Heavy metals were not accumulating in cottontail rabbits or white-
    footed mice inhabiting irrigated sites.  These studies should be con-
    tinued over longer time periods and in areas where industrial wastes
    create higher risks concerning this hazard.
       Studies on the effect of wastewater renovation on wildlife and wildlife
    habitat have been limited to only a few vegetative types and have been
    hindered by small plot size and short duration of investigations.  As lar-
    ger irrigation systems become operable, opportunities to investigate the

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    definitive relationship between habitat changes and wildlife  responses
    will arise.  Integral to these studies are investigations on  the enhance-
    ment, prevalence, and pathogenicity of disease in wildlife which may pre-
    sent a human health hazard. (AA)
33. Anthony, R.G. and R. Kozlowski. 1982.  Heavy Metals in Tissues of Small
       Mammals Inhabiting Waste Water Irrigated Habitats.  Journal of Environ-
       mental Qualities. 11:20-22.

       Concentrations of heavy metals in liver and kidney of meadow voles
    (Microtus pennsyIvanicus) and white-footed mice {Pejrmy sous leucopus) from
    wastewater-irrigated and control areas were analyzed.  Heavy metals were
    not accumulating in meadow voles inhabiting a reed canarygrass (Phalaris
    arundinacea) field irrigated with sewage effluent. In contrast, Pb and Cd
    concentrations were significantly higher in tissues of white-footed mice
    inhabiting an irrigated forested site as compared with a control forested
    site.  However, concentrations of Pb and Cd were not high enough to be
    considered toxic.  Cadmium/zinc ratios for kidneys of small mammals were
    higher than those for soils and vegetation on irrigated areas, demonstrat-
    ing potentially hazardous levels in herbivorous small mammals inhabiting
    areas irrigated with sewage effluent at application rates ranging from 5.0
    to 7.5 cm/week for 14 years. (AA)
34. Attwell, B.J. et al.  1980.  The Uptake of Phosphate by Carex spp. from
       Oligotrophic to Eutrophic Swamp Habitats. Physiologia Plantarum
       44(4):
35. AveLallemant, S. P., and J. W. Held.  1980.  Assessment of Sewage Lagoons
       as Potential Fish Culture Sites in West Central Wisconsin.  University
       of Wisconsin-Madison.  Water Resources Center.  Office of Water
       Research and Technology, Washington, DC.  92 pp.

       The potential of wastewater treatment lagoons in Wisconsin for cul-
    turing fathead minnows (Pimphales pronelas) was considered.  The efficacy
    of this treatment strategy in terms of improving effluent water quality
    by reclaiming nutrients in the form of fish biomass and reducing treat-
    ment costs by providing marketable bait fish was assessed.
       Wastewater lagoon systems located in 19 counties in west-central Wis-
    consin were assessed as potential fish culture sites during 1978 and 1979.
    Decisions were based on field measurements of temperature and dissolved
    oxygen (DO), number of lagoons, loading rates, and 24-hr, on-site cage
    bioassays with fathead minnows.  Water samples for chemical and phyto-
    plankton analyses were collected every 2 weeks from May through September
    at 10 of the facilities during 1979.
       Successful fish culture in wastewater treatment lagoons appears to
    depend on the loading rate and design capacity of the facilities consid-
    ered.  High levels of ammonia-nitrogen and low levels of DO were major
    limitations to fish culture in sewage lagoons.  Characteristics of lagoon
    systems with a high probability of culture success include moderate load-

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    ing rates, pretreatment of waste and aeration.  Fish culture in waste-
    water lagoons may provide a harvestable by-product which could help off-
    set operating costs*  (AA)
36. AveLallemant, S. P.  (In preparation).  Baitfish Culture in Wastewater
       Treatment Lagoons in Wisconsin.  Master's Thesis.  Univ. of Wisconsin.
       La Crosse.
37. Azharia Jahn, Samia.  1976.  Sudanese Native Methods for the Purification
       of Nile Water During the Flood Season,  p. 95-106, In; Biological Con-
       trol of Water Pollution.  J. Tourbier and R.W. Pierson, Jr. (Editors).
       University of Pennsylvania Press.
38. Badour, S. S., H. R. Godavari, A. Hussain, Y. C. Tai, and E. R. Waygood.
       1980.  Purification and Reclamation of Farm and Urban Wastes by
       Euglena gracilis; Photosynthetic Capacity, Effect of pH, Temperature,
       Acetate, and Whey.  Environmental Pollution 23(3):179-215.

       The purpose of this study was to provide an efficient, inexpensive
    algal system for the purification of urban and farm wastes, and the
    conversion of organic and inorganic nutrients into a mono-algal product
    that could be standardized as a feedstuff or as a fertilizer, with the
    remaining water fit for aquaculture or human consumption.  The method and
    results are described in detail.  The system proposed could be useful for
    purifying and reclaiming wastes by Euglena gracilis, especially for the
    dairy and meat-packing industries, and in increasing the organic matter of
    laterite soils, provided that wastes are readily accessible. (AL)

39. Bagnall, L. O.  1979.  Resource Recovery from Wastewater Aquaculture,
       pp. 421-439.  In R. K. Bastian and R. C. Reed (Project Officers),
       Aquaculture Systems for Wastewater Treatment: Seminar Proceedings and
       Engineering Assessment.  EPA-430/9-80-006.  U.S. Environmental Pro-
       tection Agency, Office of Water Program Operations, Municipal Con-
       struction Division, Washington, DC.  485 pp.

       Removal and recycling of the nutrients and other components in the
    wastewater aquaculture system are completed by harvesting, processing and
    utilizing the aquatic organisms in which the nutrients are collected.
    Aquatic species grown in wastewater have been experimentally used for
    food, feed, fiber, fertilizer and energy.  Some of the processes and pro-
    ducts appear to be technologically and economically feasible on an opera-
    tional scale.
40. Bagnell, L.O. et al.  1974.  Feed and Fiber from Effluent-Grown Water
       Hyacinth, pp. 116-141.  In: Wastewater Use in the Production of Food
       and Fiber-Proc.  U.S. Environmental Protection Agency, Ada, OK.

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  41.  Bajer,  D.C.  and W.B. Fryer.  1973.  Undesirable Plant Response With Sewage
         Irrigation.   J.  Irr. Drain.  Div. Am. Soc. Civil Eng. 99:133-141.
  42.  Ball,  R.C.   1977.  The Michigan State University Management Project—A
         Facility for Research in Recycling Treated Wastewater.  p.205-256.
         In; F.M. D'ltri (ed.)  Wastewater Renovation and Reuse.  Marcei Dekker,
         Inc.,  New York
  43.  Baker,  F.C.   1910.  The Ecology of the Skokie Marsh Area, with Special
         Reference to the Mollusca.  Bulletin of the Illinois State Laboratory
         of Natural History  8(4):441-499.
  44.  Banus,  M.D.,  I.  Valiela, and J.M. Teal.  1975.  Lead, Zinc, and Cadmium
         Budgets in Experimentally Enriched Salt Marsh Ecosystems.  Estuarine
         and Coastal Marine Science  3:421-430

         The amounts of lead, zinc and cadmium were measured in various compo-
      nents of a salt-marsh ecosystem experimentally treated at two levels with
      a metals-containing fertilizer.  Lead entering a salt marsh is largely re-
      tained by the surface sediments but 6-8% is taken up by marsh grasses.
      Lead in grass tops is exported as detritus to deeper waters but this loss
      was only about 3-4% of the entering lead.  About 2% of the zinc at both
      levels of addition was exported in grass detritus while 16 and 45% were
      lost by undetermined mechanisms.  About 80% of 15 mg m~2 year"1 of
      cadmium added was retained in the sediments but at a dosage of 43 mg m~2
      year"^, 65% of the added cadmium left the plot, probably in dissolved
      form.  The nitrogen added in conjunction with the metals resulted in in-
      creased movement of these metals into coastal waters due to increased pro-
      duction of grasses and increased metals contents of the grass tops. (AA)
  45.  Bardach, J.E., J.H. Ryther, and W.O. McLarney.  1972.  Aquaculture, the
         Farming and Husbandry of Freshwater and Marine Organisms.  John Wiley
         & Sons, Inc., New York.  868 pp.
1066.  Barton,  J.M. 1983.
  46.  Barth,  C.L., and J.H. Bond.  1975.  Water Management in Livestock Waste
         Handling Systems.  Clemson Univ., SC.  Water Resources Research Inst.
         Office of Water Research and Technology, Washington, DC.

         The  potential for minimizing the use of water in several animal waste
      treatment and storage procedures was evaluated.  Laboratory units simu-
      lated the operation of aerobic lagoons, anaerobic lagoons, and storage for
      diluted swine feeding floor waste under conditions of controlled tempera-
      ture, lighting,  loading rate,  and detention time.  Anaerobic lagoon simu-
      lators  achieved volatile solids (VS) reduction rates of 75.8% at 24°C and
      200 days detention time and as little as 22.1% at 10°C and 100 days deten-

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    tion time.  Degradation of VS was generally high in the loading  rate  range
    of 50 to 400 g VS/day/cu m and sludge storage efficiency was also high in
    that range.  Liquefaction was a significant factor in the storage
    simulators with reductions of total solids by as much as 69%.  Survival  of
    the swine pathogens Sa_lmpnel_la cholerae-suis and Salmonella tyjahimurium  in
    laboratory simulators of swine manure lagoons was determined and compared
    with the survival of the indicator organism Escherichia coli and fecal
    coliforms.  The heaviest populations of the pathogens died off in 33  days.
    A cause of Salmonella, die-off was proposed.  A mathematical model was
    developed to describe the operation of the anaerobic animal waste lagoon
    and compared with relevant data which characterized the lagoon simulators.
    (NT)
47. Barret, G.W., G.M. Van Dyne, and E.P. Odum.   1976.  Stress Ecology.   Bio-
       Sciences 26(3):192-194.
48. Bartlett, M. S. , L. C. Brown, N. B. Hanes, and N. H. Nickerson.   1979.
       Denitrification in fresh water wetland soil.  J. Environ. Qual.  8(4):
       460-464.

       The application of secondary treated wastewater to  freshwater  wetlands
    received recent attention as a tertiary treatment method.  The  fate of
    nitrate nitrogen in such applications is  of concern because of  the
    potential for leaching into ground and surface water supplies.  Nitrate
    removal in wetland soilwater suspensions  was  studied using a Warburg
    respirometer.  All important pathways of  nitrate reduction were accounted
    for using a N mass balance.  Of the nitrate,  90-95% added to wetland
    soil-water suspensions is reduced to nitrogenous gases, with little or no
    transfer of nitrate to ammonia or organic nitrogen fractions.  (BA)
49. Bartsch, A. F.  1956.  Biological and chemical aspects of  organic  waste
       lagoons, pp. 61-86.  In Land Disposal of Liquid Waste.  Robert  A.  Taft
       Sanitary Engineering Center, Cincinnati, OH.

       Growing popularity of waste stabilization ponds has created new
    interest in the pond mechanism and factors affecting  it.   Mode of  action
    and impact of these factors influence design and  resulting pond accom-
    plishment.  Of importance are photosynthetic oxygenation;  interrelation
    of light penetration, algal density, and depth; the effect of  temperature
    upon BOD rate, DO saturation level, and photosynthesis; and  the manner in
    which seasonal forces are superimposed upon daily fluctuation  in pond
    characteristics.  The relative influence of ice cover and  loading  pattern
    and the responses that follow are pointed out.  (AA)
50. Bastian, R.K.   1979.  EPA Interest  in  the Potential  for Wetland Treatment
       of Wastewater.  In;  Sutherland  and Kadlec  (eds.),  Wetland Utilization
       for Management of Community Wastewater.  Abstracts of a  Conference held
       July 10-12 at Higgins Lake, MI.

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       Wetland treatment  systems  do  appear  to  have  a place  as  an alternative
    to conventional technologies  used  in municipal  wastewater  treatment.  This
    is especially true for  small  communities faced  with  upgrading existing
    facilities and increasing wastewater treatment  requirements.
       Natural wetlands have inadvertently  served as natural waste treatment
    systems for centuries.  However/ in recent years marshes,  swamps,  bogs,
    and other wetland areas have  been  successfully  utilized as managed
    "nutrient sinks" for polishing partially treated effluents under rela-
    tively controlled conditions.  Constructed wetlands  have also been in-
    vestigated and designed to meet  specific project conditions while  pro-
    viding new wetland areas that also improve available wildlife habitats
    and provide the other numerous benefits of wetland areas.
       Caution should be taken, however, to avoid negative  public, government-
    al and/or scientist reactions that may be  raised when proposing wetland
    treatment systems.  The potential  for impacting biotic  communities, espe-
    cially when involving natural wetlands in  wastewater management, must be
    recognized and appropriate management practices and  adequate monitoring
    implemented to avoid potential ecological  problems from developing.   After
    all, the overloading of our Nation's waterways  with  the wastes of  civili-
    zation has been the major environmental reason  for constructing and oper-
    ating publicly owned wastewater  treatment  works in the  first  place.
       The recent research and project development  work  that has  been  under-
    taken in Michigan, Florida, New York, California, and other areas  on  wet-
    land treatment systems has helped  draw more attention to the  potential
    utility of these low cost and low  energy consuming approaches for  waste-
    water treatment.  Such natural biological  treatment  systems  should become
    increasingly important to EPA and  local communities  in  developing  the best
    practicable systems to meet future site specific wastewater treatment (and
    water reclamation) requirements.  The future of wetland systems as an
    innovative/alternative technology for municipal  wastewater treatment
    should be a bright one, although it could  be greatly influenced by public
    opinion as well as concerns expressed by governmental officials and scien-
    tists which has certainly been true in the past for  the acceptance of
    various "land treatment" technologies.  (AA)
51. Bastian, R.K.  1981.  EPA's Role and Interest in Using Wetlands  for Waste-
       water Treatment, pp. 325-336.  In; B. Richardson  (ed.).  Selected Pro-
       ceedings of the Midwest Conference on Wetland Values  and Management.
       Minnesota Water Planning Board.  St. Paul.  660 pp.

       Wetland treatment systems involving natural or artificial wetlands
    appear to be very promising as a low cost, energy efficient alternative to
    certain conventional technologies used in municipal wastewater treatment.
    This is especially true for small communities faced with upgrading or ex-
    panding existing treatment facilties.
       Both freshwater and saltwater wetlands have inadvertently served as
    natural waste treatment systems for centuries.  However, in recent years a
    number of marshes, swamps, bogs, and other wetland areas in various parts
    of the country have been effectively utilized as managed natural biologi-
    cal systems to assist in wastewater treatment.  Artificial wetlands also
    have been created and managed to meet specific pollution control require-
    ments in an environmentally acceptable, cost-effective and energy effi-
    cient manner while effectively recycling the nutrients, organic matter and

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      other wastewater constituents,  improving available wildlife habitat, sta-
      bilizing stream flows,  recharging ground water,  etc.  Therefore, such man-
      aged wetland wastewater treatment systems represent a logical extension of
      the basic land treatment recycle/reuse concepts  which have been strongly
      encouraged by the Congress and EPA.
         At the same time,  the potential for wastewater treatment projects to
      harm biotic communities in existing wetlands must be recognized.  Past in-
      cidents, where wetlands were used as a handy place to dispose of wastes
      and resulted in serious environmental problems,  have produced strong re-
      sponses from environmental groups, government agencies, and members of the
      scientific community.   Their concerns and reactions have lead to a basic
      desire to protect existing wetlands from all outside influences.  The need
      for wetland preservation should be addressed whenever wetlands wastewater
      treatment systems are proposed.  These concerns  and the ecological charac-
      teristics of wetlands suggest that appropriate management practices and
      adequate monitoring,  as well as proper regulation and control of projects
      using wetlands for wastewater treatment, must be developed and implemented
      to avoid potential ecological problems or serious project opposition from
      developing.
1067.  Bastian,  R.K.  and J. Benforado.  1983.
  52.  Bastian,  R.  K.,  and R.  C.  Reed (Project Officers).  1979.  Aquaculture
         Systems for Wastewater Treatment: Seminar Proceedings and Engineering
         Assessment.  EPA-430/9-80-006.  U. S. Environmental Protection Agency,
         Office of Water Program Operations, Municipal Construction Division,
         Washington, DC.  485 pp.

         This publication contains an engineering assessment and the proceed-
      ings of a seminar held at the University of California-Davis on 11-12
      September 1979,  on the use of aquatic systems for the treatment of
      municipal wastewater.  Case studies drawn from throughout the United
      States are used to illustrate the engineering, design, operation, and
      management of various wastewater aquaculture systems, including projects
      involving wetlands processes, aquatic plant processes, and combined
      aquatic processes.  The potential recovery of energy and resources also
      is considered. (AA)
  53. Bayley, S. E., J. Zoltek, Jr., and F. L. Boyt.  1975.  Removal of
         Nutrients from Treated Municipal Wastewater by Wetland Vegetation.
         Final report to the City of Wildwood, Florida.  Project No. 245-N34.
         Department of Environmental Engineering Sciences, Univ. of Florida,
         Gainesville, in cooperation with Michaels-Stiggins Incorporated,
         Orland, FL.  51 pp. plus appendices.
  54.  Bayley, S.E.  1983.  The Effect of Natural Hydroperiod Fluctuations  on
         Fresh Water Wetlands Receiving Added Nutrients.   (Draft Manuscript).
         In;  Ecological Considerations in Wetlands Treatment of Municipal
         Wastewaters, Proceedings of a Workshop, June 23-25, 1982, University
         of Massachusetts, Amherst MA.  US Fish and Wildlife Service  and US
         Environmental Protection Agency.

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55. Bedinger, M.S.  1978.  Relation Between Forest Species and Flooding.
       p. 427-435,  In;  Greeson, P.E., J.R. Clark, and J.E. Clark (Editors),
       Wetland Functions and Values:   the State of Our Understanding.   Ameri-
       can Water Resources Association, Minneapolis, MN.
56. Beeftink, W.G.  1977.  Salt Marshes, pp. 93-121.  In;  R.S.K.  Barnes  (ed.)
       The Coastline.  John Wiley and Sons, New York.

       In temperate regions, salt marshes are found in and around  river
    mouths, bays, Wadden areas, lagoons, and on coastal plains protected  by
    sand- and shingle-spits.  During marsh genesis, sedimentation  of  clay and
    silt takes place especially in the basins while coarser particles  are de-
    posited on the creek banks.  The roost common marsh use is grazing—usually
    by sheep, cows, and horses.  Although mostly limited to the higher marshes
    and coastal dune-slacks, grazing may take up vast areas.  Grazing  includes
    selective cutting and removal of plant parts, local manuring,  and  tramp-
    ling of the vegetation.  The latter also leads to compaction of the top-
    soil, and, more intensified, to destruction of the turf-layer.  Drainage
    of marshes is also another form of land reclamation.  It results in a low-
    ering of the water table and thus in changes in habitat, flora, and fauna.
    Transversing with ditches is harmful to the geomorphological development,
    for the natural watercourses may lose their function.  Embankment  is  one
    of the most serious impacts on salt marshes.  In industrial!1zed areas,
    marshes are threatened by a complex array of man-made detritus, litter,
    and chemical substances derived from agricultural wastes, fertilizer  or
    trash and urban sewage.
57. Beeston, M. D.  1971.  Decapod Crustacean and Fish Populations  in
       Experimental Marine Ponds Receiving Treated Sewage Wastes.   Univ. of
       North Carolina, Inst. of Marine Sciences, Chapel Hill.
58. Bender, M. E., and D. L. Correll.  1974.  The Use of Wetlands as Nutrient
       Removal Systems, Chesapeake Research Consortium Pub. No. 29.  Univ. of
       Maryland, College Park.

       Two different methodologies were used to investigate the effectiveness
    of marshes as nutrient removal systems in Chesapeake Bay.  The first
    method employed fertilization or loading experiments in combination with
    tracers, while the second measured natural flux at two undisturbed study
    sites.  Results showed that regularly flooded tidal marshes should not be
    considered as sinks for nitrogen or phosphorus forms.  Although incoming
    particulate nitrogen was transformed in the marsh and exported to the
    estuary as ammonia and dissolved organic nitrogen, there was little, if
    any, net loss of available nitrogen and phosphorus in the marsh.  The
    highest exports of dissolved inorganic phosphorus and ammonia occurred
    during the summer.  High irregularly flooded salt meadow hay (Spartina
    patens) marshes appear to have some capacity for phosphorus removal. How-
    ever, it was estimated that a loading of 29,000 gallons of secondary sew-
    age per day would saturate the capacity of an acre of high marsh in 45

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    days, and once loaded to the capacity the acre could not be expected to be
    reused for many years.  It is concluded that utilization of marshes of any
    type as nutrient removal systems is highly questionable.
59. Bender, M. E., and D. L. Correll.  1974.  The Use of Wetlands as Tertiary
       Treatment Systems.  Report No. NSF-RA-E-74-033.  National Technical
       Information Service publication PB-241002.
60. Beneman, J. R. et al.  1977.  Cultivation on Sewage of Microalgae Harvest-
       able by Microstrainers.  Sanitary Eng. Res. Lab., University of Cali-
       fornia, Richmond, CA.
61. Beneman, J. R. et al.  1978.  Large-scale Freshwater Microalgal Biomass
       Production for Fuel and Fertilizer.  Sanitary Eng. Res. Lab., Univer-
       sity of California, Richmond, CA.
62. Benforado, J.  1981.  Ecological Considerations in Wetland Treatment of
       Municipal Wastewater, pop. 307-324.  In;  B. Richardson (ed.),  Selected
       Proceedings of the Midwest Conference on Wetland Values and Management.
       Minnesota Water Planning Board. St. Paul.  660 pp.

       The use of natural and artificially created wetlands for wastewater
    discharge or treatment offers potential to reduce costs, conserve  energy,
    and reclaim wastewater constituents, when compared with conventional
    "high technology" sewage treatment.  Benefits can include preservation of
    open space, potential for wildlife enhancement, flow stabilization, and
    more.  However, ecological problems should be identified early  in  the
    development of wetland/wastewater technology.  Technical issues  include:
    health concerns for both humans and wildlife (e.g., viral, bacterial, and
    other pathogenic organisms, ground and surface water contamination, nui-
    sance insects); food chain effects of toxic substances  (e.g. biomagnifica-
    tion of heavy metals and synthetic organic chemicals, effect of  surfact-
    ants on invertebrate populations); adverse community changes (e.g. accel-
    erated succession, eutrophication, species changes, etc.); effects on fish
    and wildlife values (e.g., shellfish contamination, botulism in  waterfowl,
    effect of chlorine compounds on fish, beneficial effects such as habitat
    enhancement); effects on other wetland functions and values (e.g., flood
    control, aesthetics, recreation); and long-term changes (e.g. cumulative
    effects).
       Developing a sound policy on the use of wetland treatment systems is
    vital.  Management guidelines and water quality standards must be  devel-
    oped that will allow for treatment, yet protect wetland functions  and
    values.  Four areas of study — engineering design constraints/impact
    pathways/ecological effects/management potential —  provide a  framework
    for developing such a policy.  It seems prudent at this time to  limit the
    general application of wetland treatment technology to  artificially con-
    structed wetlands and (in appropriate circumstances) to highly  degraded

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      natural wetlands; in the latter case, the overall objective should be  to
      restore and enhance the wetland.  Management of natural wetlands should
      optimize a combination of wetland values, rather than maximize  sewage
      treatment capability.
1068. Benforado, J.   1983.
  63.  Bennett, C.L.  1979.  The Concerns of a Public Servant When Secondary
         Treated Effluent is Spread Across a Wetland.  In;  J.C. Sutherland and
         R.H. Kadlec (eds.), Wetland Utilization for Management of Community
         Wastewater, Abstracts of a Conference held July  10-12 at Higgins Lake,
         MI.

         As an ecologist concerned with lifestyles, environmental stability,
      etc., I well realize the problem involved with disposal of human waste and
      a system of recycling is the solution we feel most  appropriate, but until
      such a program is instituted it seems a likely prospect to put partially
      treated effluent onto peat lands or other forms of  bogs, marshes, and
      possibly even some flood plains.  These areas, by their nature, are
      usually highly productive in all forms of wildlife  and are used at differ-
      ent times of the year and in varying degrees by both wildlife and people.
      Our concerns are (1) public health, of course (2) changes in both flora
      and fauna that may occur, (3) deleterious effects to either flora or
      fauna, (4) to what extent the area can absorb the high level nutrients for
      a given period of time, (5) the watersheds involved, (6) maintaining the
      areas open to public use and not have them restricted because of material
      coming through with the effluent, (7) assurances that products coming off
      from the marsh remain usable, able to be handled, or edible, and  (8)
      assurances that after the areas are saturated and pumping has stopped on
      those areas that they are still usable by the public, productive, and op-
      erate in an ecosystem fashion.  In other words, we're concerned about the
      effect on plants, animals, substrate, and people.   Disposal of municipal
      waste and industrial waste is an immediate problem  and needs to be
      resolved, but industrial waste should not be put on a marsh even under
      experimental conditions.  For handling municipal wastewater, wetlands
      appear to be a possible partial solution, but research efforts must be
      maintained to assure us that we are not taking a shallow-sighted view and
      end up with an even larger problem.  I get scared every time we push our
      environmental system another notch up the scale.  The past track record
      usually ends up with, "We did it wrong and now we must pay."   (AA)
  64.  Bently,  E.M.  1969.   The Effect of Marshes on Water Quality.  Ph.D. Diss.
         University of Wisconsin, Madison.
  65.  Berg,  G.  1966.   Virus Transmission by the Water Vehicle.  II.  Virus
         Removal by Sewage Treatment Procedures.  Health Library Science 2:90.
  66.  Bevis,  F.B.   1979.   Ecological Considerations in the Management of Waste-
         water-engendered Volunteer Wetlands.  The Michigan Wetlands Conference,
         July 10-12,  Higgins Lake, MI.  19 pp.

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       This is a collection of eighteen handouts used by Dr. Bevis in his
    presentation at the Michigan Wetlands Conference at Higgins Lake,
    Michigan.  Data from the Vermontville, Paw Paw, and Kinross volunteer
    wetland treatment sites were compiled and compared in the tables and
    figures. (EPA)
       The phenology, phytosociology, productivity, nutrient levels, and wild-
    life values of the Vermontville, Michigan, wastewater-engendered volunteer
    wetlands were measured for the period June 1978-June 1979.  Results of
    this work are reported here as:

    1)   Seasonal growth and development of the dominant wetland species,
        Typha latifolia L. (Robust Cattail) and T. angustifolia L._ (Narrow-
        Leaved Cattail),
    2)   Vegetation descriptions of eight (8) different volunteer community
        types occurring in the wetlands,
    3)   Biomass accumulation, potential above-ground plant harvest, and decom-
        position of the vegetation,
    4)   TKN and TP accumulation in the vegetation, potential removal of these
        nutrients from the system via standing crop harvest,
    5)   Wildlife populations and utilization of the wetlands.

    Observations on the above features from the PawPaw, Michigan, wastewater/
    wetlands system support the results of the Vermontville project.  (AA)
67. Bsvls, F. B., and R. H. Kadlec.  1979.  Effect of Long-Term Discharge of
       Wastewater on a Northern Michigan Wetland.  In J. C. Sutherland and R.
       H. Kadlec (eds.), Wetlands Utilization for Management of Community
       Wastewater.   Abstracts of a conference held 10-12 July 1979 at Higgins
       Lake, MI.

       Apparently the effluent discharge from Kincheloe/Kinross Air Force
    Base, initiated in the late 1940's, has converted portions of a diverse
    acid/peat system to A more specific monoculture cattail marsh system.  The
    degree of this  ecological/habitat change in a sensitive peatland ecosystem
    is being documented by observations on the composition of the vegetation;
    plant community distribution patterns; successional changes in the vegeta-
    tion; and flow paths of the wastewater traceable through the wetland by
    following the cattail component community assemblages evident in the peat-
    land.
       Mapping and phytosociological descriptions of the vegetation have been
    determined in the field.  The extend (acreage) of the various plant commu-
    nities and their pattern of occurrence in the peatland are being deter-
    mined from 1939, 1952, 1964, 1969, 1974 and 1978 aerial photographs. The
    study of the progress (rate) of community conversion(s) over the 25 years
    from a diverse, species-rich natural peatland to the cattail monoculture
    should help to establish appropriate guidelines for the effective utili-
    zation of such wetlands in wastewater polishing.  (AA)
68. Bierei, G. R., G. W. Wood, and R. G. Anthony.  1975.  Population response
       and Heavy Metals Concentrations in Cottontail Rabbits and Small
       Mammals in Wastewater Irrigated Habitat, pp. 1-9.  In G. W. Wood et
       al. (eds.), Faunal Response to Spray Irrigation of Chlorinated Sewage

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    Effluent.  Institute for Research on Land and Water Resources Research
    Publication 87.  The Pennsylvania State University, University Park,
    PA
69. Bingham, F. T., A. L. Page, R. J. Mahler, and T J. Ganje.   1975.   Growth
       and Cadmium Accumulation of Plants Grown on a Soil Treated with a
       Cadmium-enriched Sewage Sludge.  Toxicity.  J. Environ. Qual.
       4:207-211.

       Corn, wheat, rice, field bean, soybean, spinach, lettuce, curlycress,
    carrot, turnip, radish, tomato, and squash plants were grown to commer-
    cial harvest stage using as the substrate, soil pretreated with a  muni-
    cipal sewage sludge (1%) containing variable amounts of CdSO4 up to 640
    ug Cd/g soil.  Observations included injury symptoms, yield decrement, Cd
    level of diagnostic tissue, and Cd and Zn content of harvested produce.
    Cadmium sensitive plants such as spinach, soybean, curlycress, and lettuce
    were injured by soil Cd levels of 4-13 ug Cd/g soil; whereas, tomato and
    cabbage tolerated soil levels of approximately 170 ug Cd/g soil without
    exhibiting injury symptoms.  Rice was tolerant at all levels tested. Leafy
    plants such as lettuce, spinach, and turnip greens (tops) accumulated  175
    to 354 ug Cd/g; whereas, fruit and seed tissue of plants under comparable
    treatment, with the exception of soybean, accumulated no more than 10 to
    15 ug Cd/g tissue.  The concentrations for soybean extended up to  30 ug
    Cd/g tissue.  Paddy rice exhibited no ill effects for soil Cd treatments
    up to 640 ug Cd/g soil.  Grain of plants under the 640 ug Cd/g treatment
    contained 2 ug Cd/g tissue.  DPTA-extractable Cd correlated (r=0.99) with
    the level of CdSO^ added to the substrate.  (AA)
70. Bingham, F. T., A.L. Page, R.J. Mahler, and T.J. Ganje.  1976.  Cadmium
       Availability to Rice in Sludge-amended Soil Under "Flood" and  "Non
       Flood" Culture.  Soil Sci.  Soc. Am. J. 40: 715-719.

       Rice plants (Oryza sativa. var.  'Colusa') were grown to maturity in pots
    containing a soil (Domino silt loam, pH 7.5, Xerpllilc calciprthi d) amend-
    ed with 1% sewage sludge enriched with variable amounts of CdSO,  ranging
    up to 640 ug Cd/g.  Two sets of soil cultures were used, one for  rice
    under continuous flood and the other under nonflood conditions.   Grain
    production for rice under flood management was relatively unaffected by
    the Cd treatment; 25% yield decrement was associated with a treatment of
    320 ug Cd/g.  Under nonflood management, however, a comparable decrement
    in grain production was observed with a treatment of only 17 ug Cd/g. The
    Cd content of mature leaves at early flowering varied from approximately
    0.3 ug Cd/g for the controls to 2.8 ug Cd/g for plants receiving  the high-
    est Cd treatments.  Leaf Cd values were slightly higher under the nonflood
    culture.  The Cd content of the grain under nonflood management was
    approximately 55% greater than than of grain under flood management.  The
    Cd content of mature leaves correlated with grain production (r=0.872***)
    for rice produced under either flood or nonflood culture.  Chemical analy-
    sis of saturation extracts revealed greatly reduced Cd concentrations in
    soil solutions under flood management which may account for the greater
    tolerance of the cultivar to soil Cd under flood culture.  This reduced
    availability of Cd in flooded soils is attributed to precipitation of
    CdS.  (AA)

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  71. Bisogni, J. J., Jr.,  and A.  W.  Lawrence.   1975.   Kinetics of Mercury
        Methylation  in Aerobic  and Anaerobic Aquatic  Environments.  Journal
        Water Pollution  Control Federation  47:135-152.

        The microbial transformation of inorganic mercury to organic mercury
      (monomethyl and dimethyl mercury)  was studied under a variety of condi-
     tions.  A  kinetic model  was  proposed and  experimentally verified to des-
     cribe the  rate  of microbial  methylation as a function of the followoing
     parameters: relative  microbial  activity;  biochemical availability of in-
     organic mercury; total inorganic mercury  concentration; and redox poten-
     tial.  The model and  the experimental results demonstrated that mercury
     may  be methylated under  anaerobic and aerobic conditions and that the
     predominant methylation  product near neutral pH  is  monomethyl mercury.  It
     was  also found  that large  amounts of inorganic mercury may be released
     from an aquatic system as  volatile elemental mercury.  Application of the
     kinetic model to natural aquatic benthic  regions and biological wastewater
     treatment  processes is discussed in terms of possible control of the meth-
     ylation phenomenon.  (AA)
  72.  Bitton,  G.,  G.  E.  Gifford,  C.  Monteith,  and O.  Pancorbo.  1977.  Virus
         Removal  in Marine Aquaculture Systems.   Revue Internationale
         d'Oceanographic Medicale 48:47-52.

         The possibility of removing polioviruses from sea water,  secondary
      sewage effluent and mixtures of the two was investigated using a magnetic
      separation  process.   The object was to remove poliovirus without reducing
      the nutrient content of the water-sewage mixture, and the growth of two
      types of marine diatom in the filtered effluent was tested.  It was con-
      cluded that magnetic filtration could form an effective primary step for
      removal  of  viruses in an aquaculture system without affecting the sub-
      sequent  growth  of  algae acting as primary producers in the aquatic
      system.   (AL)
  73.  Bitton,  G.,  and N.  Masterson.   1975.   Virus:  Effect of Dome Water on the
         Movement  of Poliovirus Type 1  and Bacteriophage T2 through a Sandy
         Soil  Sampled at  Sewage Dome 1, pp. 383-384.  In H. T. Odum, K. C.
         Ewel, J.  W. Ordway,  and M.  K.  Johnston (eds.), Cypress Wetlands for
         Water Management,  Recycling and Conservation.  Second Annual Report.
         Center for Wetlands.  Univ. of Florida.  Gainesville.
  74.  Bitton,  G.,  P.  R.  Scheureman,  G.  E.  Gifford, and A. R. Overman.  1976.
         Movement  of  Viruses through a Cypress Dome: Evaluation of the
         Virus-retention Capacity of the Dome Sediments and of a Loamy Sand
         Layer,  pp.  321-335.  In H.  T.  Odum, K. C. Ewel, J. W. Ordway, and M.
         K.  Johnston  (eds.), Cypress Wetlands for Water Management, Recycling
         and Conservation, Third Annual Report.  Univ. of Florida, Gainesville.
1069.  Black,  S.A.,  I.  Wile,  and G.  Miller.  1981.

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75. Blunter, K.  1978.  The Use of Wetlands for Treating Wastes - Wisdom  in
       Diversity, pp. 182-201.  In M. A. Drew (ed.), Environmental Quality
       Through Wetlands Utilization.  Proceedings of a symposium held 28
       February 2-March 1978, at Tallahassee, Florida.  Sponsored by the
       Coordinating Council on the Restoration of the Kissimmee River Valley
       and Taylor Creek-Nubbin Slough Basin, Tallahassee, PL.

       Two artificial  wetland systems consisting of linear configurations of
    a marsh-pond and a meadow-marsh-pond have been treating sewage in contin-
    uous operation for four years.  Data from the past two and one half  years
    suggest that the trapping of nutrients may be increased by increasing the
    number of biotopes in the flow sequence.
       For the entire system, the retention of phosphorus and nitrogen per
    unit area of wetland appears greater when a variety of ecocomponents are
    used in sequence than if only one type of system is used.  By increasing
    the physical and biological barriers to nutrient movement, an increase in
    the number of habitats (and ecological niches) is achieved.  This seems to
    be especially important when hydrologic flow alternates between drought
    and flood, and in a temperate climate with dramatic seasonal temperature
    differences.
       The implication for the Kissimmee watershed, beset with the problems of
    high seasonal nutrient flushes, is that a management strategy which
    includes the intermittent use of diverse wetland ecosystems can be
    expected to achieve a greater degree of nutrient retention than a strategy
    which attemps to optimize a single type of ecosystem, for example a  marsh
    alone.  Practical applications may include such simple measures as provid-
    ing ponds to alternate with marsh wetlands, or designing wetlands with is-
    land meadows in appropriate sequences.  (AA)
76. Bogaert, R. M.  Nocturnal Dissolved Oxygen and Ammonia Cycles  in  the
       Wastewater Fed Marshes of Mt. View Sanitary District.  Presented at
       the Water Reuse Symposium II.  Washington, DC.  Aug. 23-28,  1981.

       Mt. View Sanitary District has had a system of marshlands fed  entirely
    by secondary treated wastewater since 1975.  The creation, management,
    and development of the marshlands was reported by Francesca Demgen in
    1979.
       In 1979-1980, a special monitoring program was conducted a-t  the request
    of the California Regional Water Quality Control Board, San Francisco Bay
    Region (CRWQCB).  This program monitored several water quality  parameters.
    These parameters were of concern because of their effects when  the marsh
    waters are released from the marsh environment.
       Nocturnal dissolved oxygen cycles were studied during the winter,
    summer, and early fall.  There were five monitoring  stations,  one in  each
    pond, at which D. O. concentrations were recorded through the  night.
    Lowest levels averaged 4.0 mg/L in three ponds; these levels consistently
    occurred at about 8 am.  Lowest D. O. levels of about 1.0 mg/L occurred
    in cattail choked pond and in the pond which first receives the waste-
    water.
       Un-ionized ammonia levels were monitored for four weeks during the dry
    weather and for four weeks during the wet weather.   Un-ionized  ammonia
    levels averaged 0.22 mg/L at all stations during the winter monitoring.
    During the summer dry weather monitoring, un-ionized ammonia levels

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    averaged 0.210 mg/L at one station, 0.026 mg/L at a second station, and
    0.050 mg/L at a third.  Transitory levels above the 0.4 mg/L maximum
    limit allowed in receiving waters occurred once at one station.  Other
    stations occasionally reached 0.1 mg/L.  The marshlands at present have
    two fish species, carp, and mosquito fish.  Carp develop gill lesions
    when exposed to 0.1 mg/L un-ionized ammonia for periods exceeding 35
    days.
       Prime factor causing the high, transitory levels was algal photosyn-
    thesis.  This activity increases the pH of the water; since un-ionized
    ammonia concentration increases with pH, concentration rose as the day
    progressed.
77. Bollman, F. H.  1975.  The Value of Estuarine Fisheries Habitats:  Some
       Basic Considerations in their Preservation.  Proc. Conf. on Estuarine
       Pollution Control and Assessment.  Pensacola, FL.  11-13 Feb.  1975.
       EPA report 7-77-007B/1-77-007A.

       Two broad sources of degradation of fishery habitat are foreseen  as
    resulting from population growth and economic development.  These  are
    direct pollution of nutrients and toxic materials, and secondly,  physical
    alteration.  Three areas of intensified use will increase the difficulty
    in maintaining fishery resources in the estuary.  These include:  1)
    increased municipal and industrial waste loads, 2) leakage of petroleum
    and petroleum products into estuaries, 3) upstream activities affecting
    freshwater inflows.  There is an urgent need to improve EIS's so  that the
    full extent of the value is displayed for the decisionmakers.  (WE)
78. Boto, K. G., and W. H. Patrick, Jr.   1979.  Role of Wetlands in  the
       Removal of Suspended Sediments, pp. 479-489.  In P. E. Greeson, J.  R.
       Clark, and J. E. Clark (eds.), Wetland Functions and Values:  the  State
       of Our Understanding.  American Water Resources Association,  Min-
       neapolis, MN.

       One of the major functions of wetlands is the removal of suspended
    sediment from water moving through the wetlands.  Flow rate is decreased
    as the water moves more by sheet flow than by channel flow, and  the  re-
    sulting decrease in velocity and the  presence of vegetation promote  fall-
    out of suspended particles.  The effect of salt water in estuarine mixing
    is to further enhance sediment removal by flocculation of clay particles.
    Transfer of the suspended sediment and the associated dissolved  materials
    from the water column to the land surface has important consequences both
    for the quality of the water and the  properties and functions of the wet-
    land.  In areas where the land surface is subsiding, sediment removal  from
    water is essential for maintaining the marsh surface.  With increase in
    toxicant load of water reaching wetlands, they are serving more  than in
    the past as a sink for materials of known toxic effects on biota.  The
    long-term consequences of the effect  are poorly understood and deserve
    study.  (AA)

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79. Bourg, A. C. M., I. Valiela, and J. M. Teal.  1979.  Heavy metal budgets
       in salt marsh ecosystems.  Presented at the Commission of European
       Communities et al.  Heavy Metals in the Environment International
       Conf./ London.  Sept. 1979.  365 pp.

       Research report:  The consequences of enriching salt marshes with a
    sludge that contains fertilizer were examined. Marsh capacity to retain
    heavy metals present in the sludge was surveyed.  The metal load of the
    sediment increased with application of the sludge fertilizer.  The con-
    centrations of cadmium, chromium, copper, and nickel in marsh plants were
    greater than those in control plants.  Sediment cores showed a downward
    migration of metals.  No deleterious effects of the metals on plants were
    observed. (EL)
80. Bourne, G.  1976.  Water Quality Effects of Sewage Effluent on a Cypress
       Dome System.  Master's thesis, Department of Environmental Engineer-
       ing, University of Florida, Gainesville.
81. Bower, H., and R.L. Chaney, 1974.  Land Treatment of Wastewater.  Adv.
       Agron. 26:133-176
82. Boyd, C. E.  1970.  Vascular Aquatic Plants for Mineral Nutrient Removal
       from Polluted Waters.  Econ. Hot. 241:95-103.
83. Boyd, C. E.  1971.  A Bibliography of Interest in the Utilization of
       Vascular Aquatic Plants.  Econ. Bot.  25: 74-84.
84. Boyt, F. L.  1975.  A mixed hardwood swamp as an alternative to tertiary
       wastewater treatment, pp. 749-750.  In H. T. Odum, K. C. Ewel, J. W.
       Ordway, and M. K. Johnston (eds.). Cypress Wetlands for Water Manage-
       ment, Recycling, and Conservation.  Second Annual Report.  Center for
       Wetlands.  Univ. of Florida.  Gainesville.
85. Boyt, F. L., S. E. Bayley, and J. Zoltek, Jr.  1977.  Removal of nutri-
       ents from treated municipal wastewater by wetland vegetation.  J.
       Water Pollut. Control Fed. 49(5):789-799.

       A mixed hardwood swamp in central Florida, U.S.A., which has been
    receiving secondarily treated domestic wastewater from Wildwood for 20
    years, was examined to determine the effectiveness of the swamp in
    removing nutrients from wastewater.  After flowing through the experi-
    mental swamp, the nutrient concentration of the water was less than the
    concentration of nutrients found in the control swamp and in Lake

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      Panasoffkee,  the final receiving body.  Water samples throughout the
      experimental  and control swamp were examined for pathogenic bacteria.
      Wetlands may  be used as an alternative to tertiary treatment especially
      to increase nutrient removal.  (BA)
  86.  Bozko,  L.  et al.   1967.   Biological ponds as the third stage of sewage
         treatment.   Gas.  Woda Sanit. 40:201-202.
  87.  Bradford,  G.  R.,  A.  L. Page, L. J. Lund, and W. Olmstead.  1975.  Trace
         Element Concentrations of Sewage Treatment Plant Effluents and
         Sludges:  Their Interactions With Soils and Uptake by Plants.  J.
         Environ.  Qual. 4:123-127.

         Metropolitan sewage sludges and effluents were characterized in rela-
      tion to their total  and water extractable trace elements, their reactions
      with soils,  and their effects on plants.  The total concentrations of
      trace elements in sludges and effluents were highly variable depending on
      the source and were  often much higher than concentrations found in soils.
      Concentrations of Cu, Zn, Ni, Co, and Cd were consistently greater in the
      saturation extracts  obtained from sludges than those obtained from a large
      sampling of  California soils.  The effects of irrigating bean, barley, and
      tomato plants in a greenhouse sand culture experiment with diluted satura-
      tion extracts of  sludges from different sources were all adverse but vari-
      able depending on the sludge source.  Leaf samples from treated plants
      frequently contained toxic levels of B and excessive levels of one or more
      of the following elements: Cu, Mo, Ni, Co, Pb, and Cd.  (AA)
  88.  Breckenridge,  R.P.,  L.S.  Cahn, T.L. Thurow.  1981.  Biological Treatments
         and Uses of Geothermal Water as Alternatives to Injection, Idaho
         National Engineering Laboratory.  EE&G Idaho, Inc. Idaho Palls, ID.

         Results of  experiments demonstrate the ability of biological systems to
      thrive on and  accumulate elements from the geothermal effluent. Additional
      benefits can also be realized from the reuse of this water for irrigation
      or creation of fish or wildlife habitat.  Future studies are aimed at fur-
      ther quantifying the treatment capability and the biomass production
      potential of biological systems using geothermal water. By providing an
      environmentally sound alternative to injection or a way to improve fluid
      quality prior  to injection,  the competitive economic position of geother-
      mal commercialization may be enhanced.
1070.  Brennan,  K.M.   1981.
  89.  Brennan, K.M. and C.G. Garra. 1981.  Wastewater Discharges to Wetlands  in
         Six Midwestern States, pp. 285-294.  In.  B. Richardson (ed.), Selected
         Proceedings of the Midwest Conference on Wetland Values and Management.
         Minnesota Water Planning Board.  St. Paul.  660 pp.

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         An inventory was conducted of wastewater treatment facilities that dis-
      charge to wetlands in the states of Illinois, Indiana, Michigan, Minne-
      sota, Ohio, and Wisconsin.  The following information was obtained for
      each of the 96 facilities identified: location, type of facility, type of
      wastewater treatment, operational date, number of years of discharge,
      acreage of wetland, and general description of wetland.  Each wetland ten-
      tatively was classified according to the U.S. Fish and Wildlife Service
      classification system of Cowardin, et al.  (1979).
         The greatest number of discharges are to Palustrine emergent wetlands
      (principally cattail marshes).  Fifteen of the discharges are to man-modi-
      fied wetlands.  Three wastewater treatment systems have been designed to
      include the use of a nearby wetland as a tertiary treatment site.  Three
      volunteer wetlands (wetlands that formed as a consequence of a wastewater
      discharge) also were identified.  No artificial wetland treatmlent system
      is in operation within the six-state area.  (AA)
1071.  Brennan,  K.M.,  S.D.  Bath and G.L. Seegert.  In press.
  90.  Breteler,  R.J.,  I.  Valiela, and J.M. Teal, 1981.  Bioavailability of Mer-
         cury in Several Northeastern U.S. Spartina Ecosystems.  Estuarine,
         Coastal and Shelf Science  12(2): 155-166.

         Although considerable research has been done on the release of mercury
      from the sediments of salt marsh environments and on factors contributing
      to the mercury concentration of organisms, little attention has been given
      to the effect of the sediment itself on the availability of soil-bound
      mercury to biota.
  91.  Brightman,  R.S.  1976.  Benthic Macroinvertebrate Response to Secondarily
         Treated Sewage Effluent in North-Central Florida Cypress Domes.  Mas-
         ter's Thesis,  University of Florida, Gainesville.  112 pp.
  92.  Brightman,  R.,  and J.L.  Fox.   1976.  Response of Benthic Invertebrate
         Populations  to sewage Addition.  In  H.T. Odum, K.C. Ewel, J.W. Ordway,
         and M.K.  Johnston (eds.),  Cypress Wetlands for Water Management, Recy-
         cling and Conservation.   Third Annual Report. Center for Wetlands.
         Univ.  of  Florida, Gainesville.
  93.  Brinson,  M.M.   1977.   Decomposition and Nutrient Exchange of Litter in an
         Alluvial Swamp Forest.   Ecology 58:601-609.


  94.  Brinson,  M.M.   1983.   Management Potential for Nutrient Removal in
         Forested Wetlands.   (Draft Manuscript).  In; Ecological Considerations
         in Wetlands Treatment of Municipal Wastewaters.  Proceedings of a Work-
         shop,  June  23-25,  1982,  University of Massachusetts, Amherst, MA.  US
         Fish and Wildlife  Service and US Environmental Protection Agency.

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 95. Brinson, M. M., H. D. Bradshaw, and E. S. Kane.  1981.  Nitrogen Cycling
        and Assimilative Capacity of Nitrogen and Phosphorus by Riverine
        Wetland Forests.  Rept. No. UNC-WRRI-81-76.  North Carolina Resources
        Research Inst.  Raleigh.

               Labeled (15N) nitrate and ammonium were added to swamp surface
        water and their diffusion to the forest floor was followed.  Of the
        original nitrate added, 46% remained in the surface water of Tar Swamp
        and 62% in Creeping Swamp after 2 days.  Two days after ammonium
        treatments, corresponding levels were 79% and 81%.  As indicated by
        the absence of recoverable 15N in sediments following nitrate treat-
        ments, diffusion of labeled nitrate to the forest floor resulted in
        its transformation to T*2° or N2 bY denitrification.  Available
        nitrogen reserves in the sediments were depleted through ammonifica-
        tion and nitrification during annual drydown episodes, and the
        capacity for additional nitrogen assimilation by the sediments was
        renewed.  An experiment was then conducted to determine the capacity
        of sediments for sustained nutrient assimilation by adding nitrate,
        ammonium, phosphate, and secondarily treated sewage effluent to sur-
        face water in separate chambers at weekly intervals for 46 weeks.
        (AA)

 96. Brown, J. L., and R. S. Farnham.  1976.  Use of Peat for Wastewater
        Filtration - Principles and Methods.  Submitted for publication.
        Fifth Int. Peat Cong., Warsaw.
 97.  Brown, R. J. (ed.).  1978.  Water Pollution in Estuaries and Coastal
        Zones, Volume 2: A Bibliography with Abstracts.  NTIS/PS-78/1176.
        Springfield, VA.  252 pp.
 98. Brown, S.  1978.  A Comparison of Cypress Ecosystems and their Role in
        the Landscape of Florida.  Ph.D. Dissertation, University of Florida,
        Gainesville.
 99.  Brungs, W.A.,  R.W.  Carlson, W.B. Horning II, J.H. McCormick, R.L. Spehar,
        and J.D.  Yount.   1978.  Effects of Pollution on Freshwater Fish. J.
        Water Pollut.  Control Fed.  50(6):1582-1637.
100.  Bryan,  E.H., 1981.   Recycling Projects Receive "Appropriate" NSF Support.
        Biocycle  22(1):47.

        The  term  appropriate technology describes approaches to solving prob-
     lems that do not require large investments of capital resources, and the
     National Science Foundation has been instructed by Congress to support
     appropriate  technology.  The innovative concept of using wetlands for
     waste water  management is an example of an appropriate technology.  The
     foundation supported a basic study to mathematically model the wetland

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     ecosystem near the community of Houghton Lake in Michigan.  Field verifi-
     cation of the ecosystem models followed.  Then a pilot scale experiment
     was conducted to verify the ecosystem model's predictions of the effects
     that the placement of the secondary effluent from the area's waste water
     treatment plant would have on the wetland.  Now, full scale experimenta-
     tion is in progress at Houghton Lake.  The community's savings over a 20-
     year period were estimated to be $1.2 million over land application, the
     next least-cost alternative.  Other current research includes alternative
     methods and systems for curbside sorting of municipal solid wastes and a
     project to use wastes to help revegetate the areas damaged by the eruption
     of Mt. St. Helens.
101. Buck, D.H., J.B. Richard, and C.R. Rose.  1978.  Utilization of Swine
        Manure in a Polyculture of Asian and North American Fishes.  Trans.
        Amer. Fish. Soc. 107(1):216-222.

        Chinese methods of fish culture were evaluated for potential use in
     animal waste management, pollution control, and the production of pro-
     tein.  Two ponds were stock with grass carp (Ctenopharyngpdpn idella),
     silver carp (Hypophthalmichthys moilotrix), bighead carp (Aristighthys
     nobilis), carp (Cyprinus carpig), northern largemouth bass (Micropterus
     salmpides salmoides), channel catfish (Ictalurus punctatus), and the
     hybrid of bigmouth buffalo female (Ictiobus cyprjnellus) and black
     buffalo male (3^. niger).  One pond (0.127 hectare) received the total
     wastes from five growing pigs (about 39 pigs/hectare of water area), the
     other pond (0.121 hectare) received the wastes from eight pigs (66
     pigs/hectare).  Two consecutive lots of pigs were fattened during the
     study.  Over a fish-growing period of about 170 days (May to October  1975)
     the net increments in fish biomass were 2,971 kg/hectare in the 0.127-
     hectare pond and 3,834 kg/hectare in the 0.121-hectare pond.  A beneficial
     influence by the fish on water quality was indicated by continuously ade-
     quate levels of dissolved oxygen, and final biochemical oxygen demands in
     the range of 8-12 mg/liter.  (AA)
102.  Buck, J.  D.  1973.  Ecological Evaluation of Multiuse Waters Receiving
        Primary Treatment Effluent Prior to a Major Flow Increase.  University
        of Connecticut, Storrs.  Inst. of Water Resources.

        A chemical and biological study is described for a Connecticut estuary
     receiving 0.25-0.50 MGD of treated sewage effluent prior to construction
     of a secondary treatment plant with a projected flow of 5-6 MGD.  An
     adjacent, unpolluted cove was used as a control.  Three stations in each
     estuary were sampled bi-weekly from September 1970 to June 1972.
     Analyses  included temperature, salinity, dissolved oxygen, BOD, nitrate,
     and phosphate.  Counts were made of total bacteria, total and fecal
     coliforms, fecal streptococci, phytoplankton, and benthic invertebrates.
     (NT)

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103. Buckley, E. H.  Mitigation of Habitat Losses in the Estuary of the Hudson
        River:  Suggested Goals for Long Term Management.  Presented at the
        American Fisheries Society/et al. Mitigation Symposium, Fort Collins,
        July 16-20, 1979.  108 pp.

        Survey report:   A biological information base obtained during inves-
     tigation of an estuarine ecosystem of the Hudson River, NY, is used to
     determine mitigation requirements for long-term management of the
     estuary.  Habitat  creation can be combined with recreational development
     along the river to foster greater productivity and balance of species
     among the higher trophic levels.  (27 References).  (EL)
104. Burk, J. P., P. Hosier, A. Lawry, A. Lenz, and A. Mesrobian.  1973.
        Partial Recovery of Vegetation in a Pollution-damaged Marsh.  Project
        Completion Report.  Water Resources Research Center, Univ. of
        Massachusetts.   Amherst.
105. Burke, W.   1975.  Fertilizer and Other Chemical Losses in Drainage Water
        from a  Blanket Bog.  Irish J. Agric. Res. 14:163-178.
106. Burnett, M.  No Date.  Cypress Growth Response in a Sewage-Enriched
        Cypress Pond, pp. 774-782.  In H. T. Odum, K. C« Ewel, J. W. Ordway,
        and M. K.  Johnston (eds.), Cypress Wetlands for Water Management,
        Recycling, and Conservation.  Third Annual Report.  Center for
        Wetlands.  Univ. of Florida.  Gainesville.
107.  Euros, O. K.   1976.   Wastewater Reclamation Project, St. Croix, U. S.
        Virgin Islands.  Environmental Protection Agency,  Cincinnati, OH.
108.  Burton, T.  M., D.  L. King, R.  C.  Ball, and T. G. Bahr.  1979.  Utiliza-
        tion of  Natural Ecosystems for Wastewater Renovation.  Institute of
        Water Research, Michigan State University, East Lansing, MI.  153 pp.

        Michigan State  University constructed on 200 ha (500 acres) of the
     main campus, a permanent facility for the experimental treatment,
     recycle, and reuse of municipal sewage plant effluents.  The facility
     provides for the diversion of up to 7570 m^/d (2 MGD) of secondary
     effluent from an activated sludge treatment plant.  This waste flow is
     directed away from the receiving stream to an intensely managed aquatic
     and terrestrial nutrient recycling system.  The facility consists of a
     portion of  the East Lansing Wastewater Treatment Plant, a transmission
     line, four  experimental lakes, and a spray irrigation site.  A primary
     objective is to filter nutrients from the waste flow as it proceeds
     through the system by incorporating nutrients into harvestable biomass.

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        The system has been in operation with tertiary effluent for about  18
     months.  It will go on line with secondary effluent in  1976.  Biological
     activity in the aquatic system has a major impact on water quality  as
     evidenced by significantly reduced water concentrations of phosphorus,
     nitrogen, and inorganic carbon.  Much of the nutrient flow is shunted
     into harvestable plant material both in the aquatic and terrestrial
     portions of the system.  (AA)
109. Buikema, A. L., and E. F. Benfield.  1980.  Effects of Pollution on
        Freshwater Invertebrates.  J. Water Pollut. Control Fed. 52(6):
        1670-1686.
110. Buikema, A.L., E.F. Benfield and B.R. Niederlehner.  1981.  Effects of
        Pollution on Freshwater Invertebrates. J. of Water Pollut. Control
        Federation  53(6):1007-1014
111. Burk, C.J., Lauermann, S.D. and A.L. Mesrobian, 1976.  The Spread of Sev-
        eral Introduced or Recently Invading Aquatic Plants in Western Massa-
        chusetts.  Rhodora 78(816):727-767.

        Changes in the distributional status of several recently introduced or
     invading vascular plant species have been observed during the present cen-
     tury.  Water clover, Marsilea guadrifolia L. has become established since
     1945 along two miles of the Mill River in Northampton, Massachusetts, and
     successfully invaded the Arcadia Wildlife Sanctuary marsh during a period
     of disturbance.  Water-meal, Wolffia colu^nbiana (Karst) unknown in the
     area prior to 1933, is now abundant in highly eutrophic lakes and impound-
     ments which receive sewage effluents.  Water chestnut, Trapa natans 1^. has
     reached pest densities in Upper and Lower Ponds on the Mount Holyoke
     College campus in South Hadley and has escaped to form a colony downstream
     in Stoney Brook.  Cooter-grass, Cabpmba caroliniana (Gray), first detected
     in Massachusetts in 1930, has spread to several other western Massachu-
     setts sites and is the dominant submerged aquatic plant life in Lake
     Rohunta in Franklin and Worcester Counties.  Each of these species occurs
     in sites affected by some form of habitat disruption; none is yet so wide-
     spread as to be regularly encountered in freshwater systems.
112. Burns, L.A.  1978.  Productivity, Bioraass, and Water Relations in a Flor-
        ida Cypress Forest.  Ph.D.  Dissertation, University of North Carolina,
        Chapel Hill.
113.  Burton, T.M.  1981.  The Effects of Riverine Marshes on Water Quality, pp.
        139-152.   In  B. Richardson (ed.), Selected Proceedings of the Midwest
        Conference on Wetland Values and Management.  Minnesota Water Planning
        Board.   St. Paul.  660 pp.

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        Riverine marshes affect water quality in a number of ways.  These
     marshes usually receive water during floods as overflows from the river to
     the marsh.   They act as water retention basins and slowly release this
     water back  to the stream during periods of low flows*  While the water is
     retained in the marsh, part of the sediment load is deposited due to
     reduced carrying capacity of the slow moving water and entrapment of sedi-
     ments in marsh vegetation.  Much of the phosphorus, heavy metals, and
     toxic organic materials in stream water is transported by sorption on sus-
     pended sediments, and these materials are deposited with the sediments*
     The flooded anaerobic soils of the marsh also promote losses of certain
     elements to the atmosphere due to such products of microbial metabolism as
     nitrogen gas formed by denitrification, hydrogen sulfide, methane, etc.,
     as well as  products of aerobic decomposition at the thin oxidized micro-
     layer at the surface of the sediment.  Nutrient uptake and release pat-
     terns by the plant communities play a very important role in determining
     water quality outputs of the marsh.  Riverine marshes appear to be traps
     for most nutrients on an annual basis but can act as sources of nutrients
     under certain conditions or during seasons where plant nutrient uptake is
     low and decomposition is high. The processes affecting water quality in
     riverine marshes will be discussed and quantified whenever possible using
     literature  values.  Ongoing studies on the effects of a Michigan river-
     mouth marsh on water quality will be described.  (AA)
114.  Burton, T.M. and J.E. Hook, 1982.  Oldfield and Grass Management Studies
        on the Water Quality Management Facility at Michigan State University,
        p. 107-133.  In  Land Treatment of Municipal Wastewater: Vegetation
        Selection and Management.  Ann Arbor Science Publishers, Inc., Ann
        Arbor, Michigan.
115. Button, G», N. Morton and G.E. Gifford.  1976.  Effect of a Secondary
        Treatment Effluent on Movement of Viruses through a Cypress Dome Soil.
        J. Env. Qual. 5(4):370-375.
116. Burton, T.M., and D.L. King.  1979.  A Lake-land System for Recycling
        Municipal Wastewater, pp.68-75.  In  Proceedings of the 1979 National
        Conference on Environmental Engineering, sponsored by the American
        Society of Civil Engineers, held 9-11 July 1979 at San Francisco, CA.
117. Byers, G.L.  1971.  Influence of Wetlands on Quantity and Quality of
        Stream Flow.  Water Resources Center, Durham, NH.
118. Cairns, J., Jr.  1976.  Estimating the Assimilative Capacity of Water Eco-
        systems,  pp. 173-189.  In;  Sharma, R.K., J.P. Buffington, and J.T.
        McFadden (eds.)/ The Biological Significance of Environmental Impacts.
        NR-CONF-002.  US Nuclear Regulatory Commission, Washington, DC.

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119. Carlson, P. R., Jr.  1977.  Nutrients, Hydrogen Sulfide and Dry Matter
        Production of Spartina alterniflora in Three Salt Marshes.  Associ-
        ation of Southeastern Biologists Bulletin 24(2):41-42.
120. Carpenter, R. L., M. S. Coleman, and R. Jarman.  1975.  Aguaculture as an
        Alternative Wastewater Treatment System.  Okla. State Dept. of Health,
        Okla. City.  In; Tourbier, J. and R.W. Pierson, Jr. (eds.). 1976. Bio-
        logical Control of Water Pollution, Univ. of PA. Press, Philadelphia.
121. Carpenter, R. L., H. K. Malone, A. F. Roy, A. L. Mitchum, H. E.
        Beauchamp, and M. S. Coleman.  1974.  The Evaluation of Microbial
        Pathogens in Sewage and Sewage Grown Fish, pp. 46-55.  In; Wastewater
        Use in the Production of Food and Fiber-proc.  Environmental Pro-
        tection Agency.  Ada, OK.
122, Carriker, N.E. and P.L. Brezonik.  1975.  Heavy Metals, p. 372.  In;
        H. T. Odum, K. C. Ewel, J. W. Ordway, and M. K. Johnston (eds.),
        Cypress Wetlands for Water Management, Recycling, and Conservation.
        Third Annual Report.  Center for Wetlands.  Univ. of Florida.  Gaines-
        ville.

        Treated wastewater from a trailer park near Gainesville, FL was intro-
     duced into a small cypress dome.  Water flow generally was downward, but
     occasionally horizontal flow was observed.  Elevated levels of heavy met-
     als were found in the upper sediment layers-  (EP)
123. Carriker, N. E.  1977.  Heavy Metal Interactions with Natural Organics in
        Aquatic Environments.  Doctoral Dissertation, Department of Environ-
        mental Engineering Sciences, University of Florida, Gainesville.
        155 pp.
124. Carter, V., M.S.  Sedinger, R.P. Novitzki, and W.O.  Wien.   1978.   Water
        Resources and  Wetlands,  pp. 344-376, In;  P.E.,  J.R. Clark,  and J.E.
        Clark (eds.),  Wetland Functions and Values:  The State of Our Under
        standing.  American Water Resources Association, Minneapolis, MN.
125.  Cederquist,  N.   1977.   Wastewater Reclamation and Reuse Pilot Demonstra-
        tion Program for the Suisun Marsh.   Progress Report, U.S.  Department
        of the Interior, Bureau of Reclamation,  Mid-Pacific Region, Water
        Quality Branch,  Sacramento, CA.

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126.  Cederquist, N.  1979.  Using Wastewater for Duck Club Management in the
        Suisun Marsh of California.  In J. C. Sutherland and R. H. Kadlec
        (eds.), Wetland Utilization for Management of Community Wastewater.
        Abstracts of a conference held 10-12 July 1979 at Higgins Lake, MI.

        The Bureau of Reclamation in cooperation with the City of Fairfield,
     the Solano Irrigation District, and federal and state agencies has been
     investigating the use of wastewater for wetland management since 1973.
     The goal  of the study is to find any problems wastewater may cause and
     develop management alternatives for successfully using wastewater to
     assist in maintaining the brackish water characteristics of the Suisun
     Marsh. The initial study involved the use of secondary treated effluent
     from the  City of Fairfield's Cordelia treatment plant on about 10 acres of
     marsh and agriculture land from 1975 through the spring of 1978.  In 1977,
     a 5-year  study was started, using nitrified, filtered wastewater from the
     new 10.35 mgd Fairfield-Suisun subregional waste treatment facility. The
     study involves extensive monitoring of three clubs, each with 100 to 120
     acres of  ponds and limited monitoring of one club with 350 acres of ponds.
        During the first two years, one club used 100 percent wastewater, one
     used a mix of waste and slough water, and the third used slough water only
     as a control.  The 350 acre club used varying amounts of slough and waste-
     water. Operation is according to the schedule recommended by the Solano
     County Mosquito Abatement District and the Soil Conservation service.
     Under this schedule, the waterfowl ponds are flooded from October 1
     through May 30, then drained and dried from June 1 to September 30. From
     about January 20 to March 30, the ponds are drained and filled two to
     three times to leach salts from the soil.  The summer dry season is for
     mosquito, cattail and tule control.  (AA)
127. Cederquist, N.  1980.  Suisun Marsh Management Study: Progress Report on
        the Feasibility of Using Wastewater for Duck Club Management.  U.S.
        Department of the Interior, Water and Power Resources Service,
        Sacramento, CA.  45 pp.
128. Cederquist, N. W., and R. W. Martin.  Reclamation and Reuse of Wastewater
        in the Suisan Marsh, California.  Presented at the AWWA Research
        Foundation/et al. Water Reuse Symposium, Washington, DC.  March 25-30,
        1979.  685 pp.

        Technical Report:  The waste water reclamation and reuse pilot demon-
     stration program for the Suisan Marsh, Calif., was initiated in  1972 with
     the objective of evaluating the feasibility of beneficial reuse  and
     treatment of municipal wastewater with marsh management.  Existing tech-
     niques of marsh management were employed to maintain a desirable water-
     fowl habitat and agricultural irrigation in the marsh, utilizing waste-
     water as a supply.  It was found that algae ponds could be used  most of
     the year to provide water storage and treatment and treatment of waste-
     waters for supply to the irrigation marsh ponds.  No environmental
     problems in the irrigated marsh ponds were encountered.  Wastewater was
     shown to be a satisfactory source of irrigation water for pasturelands.
     (32 graphs, 3 maps).  (EL)

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129. Chabreck, R. H.  1976.  Management of Wetlands  for Wildlife Habitat
        Improvement, pp. 227-233.  Iri M. Wiley  (ed.)/ Estuarine processes.
        Vol. 1.  Uses/ Stresses, and Adaptation to the Estuary.  Academic
        Press, Inc., New York, NY.
130. Chalmers, A. G., E. B. Haines, and B. F. Sherr.   1976.  Capacity of  a
        Spartina Salt Marsh to Assimilate Nitrogen from Secondarily Treated
        Sewage.  Environ. Resource Cent. Georgia Inst. Technology, Atlanta.
        Tech. Rept. ERC-0776.  88 pp.

        Air-dried, pulverized secondarily treated sewage sludge was applied  to
     duplicate 100 sq m plots in a short Spartina high marsh near Sapelo
     Island, Georgia, at a rate of 200 g sludge, or 4 g nitrogen per square
     meter every two weeks for 12 months.  Monthly measurements were made of
     nitrogen pools in the marsh plants and soils in the experimental plots  and
     in duplicate control plots in the high marsh and in a low marsh area.
     Potential denitrification rates were also analyzed for marsh soils in the
     study sites.  (NT)
131. Chamie, J. P. M.  1976.  The Effects of Simulated Sewage Effluent on
        Decomposition, Nutrient Status, and Litterfall in a Central Michigan
        Peatland.  Ph.D. Diss. Univ. of Michigan, Ann Arbor.

        A wet peatland near Houghton Lake in central Michigan was chosen to
     test the feasibility of using a natural wetland as a tertiary system for
     handling secondary sewage effluent.  Nutrient solutions simulating sec-
     ondary sewage effluent were applied weekly from May to September during
     1973 and 1974 on an area dominated by sedge-willow and leatherleaf-bog
     birch cover types.  Litter biomass in both types showed significant
     and direct responses in retention of elemental concentrations of N, P, K,
     Na, Ca, and Mg following weekly nutrient application.  These values
     decreased over winter to approximately spring levels.  No significant
     differences for any species due to water and/or nutrient treatment addi-
     tions were detected for weight loss or nutrient concentrations of vegeta-
     tion exposed to decomposition.  Based on nutrient concentration and
     conditions used in this study, no significant changes are likely to occur
     in the amounts and rates of litter fall or decomposition of vegetation in
     the area studied.  (WE)
132. Chan, E., T. A. Bursztynsky, N. Hantzsche, and Y. J. Litwin.  1981.  The
        Use of Wetlands for Water Pollution Control.  Municipal Environmental
        Research Lab.  U. S. Environmental Protection Agency.  Cincinnati, OH.

        Wetlands such as marshes, swamps, and artificial wetlands, have been
     shown to remove selected pollutants from urban stormwater runoff and
     treated municipal wastewaters.  Wetlands have produced reduction in BOD,
     pathogens, and some hydrocarbons, and excel in nitrogen removal.  They
     have been reported to act as sinks for trace metals, phosphorus, and sus-
     pended solids.  Physical pollutant removal mechanisms in wetlands include

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     sedimentation, coagulation, chemical filtration, volatilization,
     adsorption, and chelation.  Vegetative mechanisms include absorption
     through roots, stems and leaves, filtration and chemical transformations
     in the plants•  Chemical transformations of some water borne pollutants
     also occur in sediment and the water column as a result of anaerobic or
     aerobic conditions, the presence of catalysts and reactive substances, and
     with the aid of microbial action.  Although individual plant species have
     been studied for their pollutant removal properties, the interaction of
     numerous plant and animal species in pollutant removal in a wetland is not
     well understood.
133. Chaney, R. L., and P. T. Hundemann.  1979.  Use of Peat Columns to Remove
        Cadmium from Wastewaters.  J. Water Pollut. Control Fed. 51:17-21.

        A method for reducing effluent Cd from industrial wastewaters to levels
     equal or below those normally found in domestic wastewaters was examined.
     A synthetic oxidized Cd plating solution (100 mg/1 Cd) was adjusted to
     levels of pH and carbonate which would cause very low equilibrium dis-
     solved Cd; 560 ug/1 Cd remained soluble because of slow precipitation.
     The solution was added in 1 1 increments (14 times) to 60 cm columns (4.9
     cm ID) of peat or peat plus CaCO3.  Effluent Cd was measured for each 1
     1 addition.  Cd in the peat columns was analyzed at various depths at the
     end of the leachings.  Results showed that peat was both an excellent
     physical filter of Cd precipitates and also an effective material for
     dissolved Cd.  Effluent averaged 2 ug/1 Cd for peat columns and 24 ug/1 Cd
     for peak plus CaCC^.  Column analysis showed that most of the CD was
     present in the upper few centimeters of the column.  Thus peat appears to
     be an effective and inexpensive method for removing Cd from pH and carbon-
     ate adjusted industrial wastewaters free of strong chelating agents.  (AA)
134. Chapel Hill Inst. of Marine Science.  Structure and Functioning of Estu-
        arine Ecosystems Exposed to Treated Sewage Wastes.  North Carolina
        Univ.  NTIS Com-71-00688, Springfield, VA.
135. Chatterjie, S. N., B. K. Arora, and D. R. Gupta.  1967.  Some Observa-
        tions on the Utilization of Sewage for Fish Culture in Oxidation
        Ponds.  Environ. Health 9:156-161.
136. Chen, K. Y., C. S. Young, T. K. Jan, and N. Rohatgi.  1974.  Trace Metals
        in Wastewater Effluents.  J. Water Pollut. Control Fed. 46:2663-2675.

        Because of the nature of its primary and secondary treatments and the
     discharge of wastewater and sludge effluents, the Hyperion Treatment
     Plant, Los Angeles, CA, was selected for an intensive study to character-
     ize trace metals in the effluents of different treatment processes.  The
     chief concern of the study was the partition of trace metals into the  dis-
     solved and particulate phases and the size distribution of the particulate

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     borne fractions in wastewater effluents and digested sludge.  With  the
     exception of Ni, Mn, and Pb, secondary treatment was found to be moderate-
     ly effective in removing trace metals in the particulate fraction.  On a
     dry weight basis, trace metals in the particulate fractions of wastewater
     effluents and digested sludge were found to be within a narrow range. (AA)
137. Cheng, P. H.  1975.  A Study on the Application of Biogrowth Sheets to
        Improve Lagoon Effluent Quality.  Univ. of Missouri, Rolla.  Dept. of
        Civil Engineering.  Office of Water Research and Technology, Washing-
        ton, DC.

        Wastewater stabilization lagoons have been employed in small commun-
     ities where available land and favorable climatic conditions allow for
     utilization as an economical secondary treatment process.  Because of
     problems associated with the discharge of algae in the effluent, the
     traditional lagoon may require either some process modifications or
     additional effluent polishing processes in order to meet local water
     quality standards.  The purpose of this study was to investigate the
     possibility of increasing attached algal growths on the surfaces of
     experimental biogrowth sheets to decrease algal discharges and improve
     the removal efficiency of organic materials as measured by Chemical
     Oxygen Demand.  (NT)
138. Chesapeake Research Consortium Inc., Baltimore, MD.  1976.  Five Year
        Program Assessment, 1970-1975.  National Science Foundation, Wash-
        ington, DC.  50 pp.

        In 1970, the Office of Interdisciplinary Research Relevant to Problems
     of Our Society (IRRPOS) of the National Science Foundation made grants to
     the Johns Hopkins University, the University of Maryland, and the Vir-
     ginia Institute of Marine Science for a research planning study of the
     Chesapeake Bay.  The report's principal recommendation was the esta-
     blishment of the Chesapeake Research Consortium (CRC) consisting
     initially of the three above-named institutions.  Early in 1972, these
     institutions were formally joined by the Smithsonian Institution, which
     up to that time had been participating in the discussions on an obser-
     vational basis.  The CRC's research programs in the areas of wetlands,
     water quality, and wastewater are reviewed in this publication.  The
     educational role of the CRC is discussed.  (NT)
139. Chestnut, A. F.  1971.  Structure and Functioning of Estuarine Ecosystems
        Exposed to Treated Sewage Wastes.  Univ. of North Carolina, Inst. of
        Marine Sciences, Chapel Hill.  345 pp.
140. Chitty, N., and C.  W. Davis (eds.).  1972.  The Effects of the Discharge
        of Secondarily Treated Sewage Effluent into the Everglades Ecosystem.
        Sea Grant Spec.  Bull. 6.  Univ. of Miami, Miami, FL.

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141.  Cody, T.  E., V.  J.  Elia, C.  S.  Clark, and R.  T. Christian.  1979.
        Integrated Use of Bioassays  and Chemical Analyses to Evaluate the
        Quality of Reuse Water, pp.  2230-2238.  In American Water Works
        Association Research Foundation, Proceedings of the Water Reuse
        Symposium.  Held 25-30 March at Washington, DC.  Denver, CO.
142.  Coldrick, J.   1975.   Sewage as a resource.  New Scientist 68:276.

        Technical  Feature:   Physicists at the Wessex Authority are working on
     ways to minimize the economic reclamation of useful products from sewage.
     They have been successful in recovering industrial water, sludge gas, and
     soil conditioners.   In the future they want to develop processes for
     recovering proteins, fine chemicals, and animal feed supplements.  A pro-
     cess for using sludge  gas for vehicle propulsion is described.  In the
     Middle East and Far  East, domestic waste and sewage are used to increase
     rural fish production.  The nutrients in such wastes increase the produc-
     tivity of fish ponds.   The few attempts to use this technique in Europe
     have succeeded in raising annual fish production rates.  This shows how a
     new cycle can be developed:  the conversion of wastes into palatable
     protein via fish rearing.  (2 photos).  (EL)
143. Cole, D. P.  (ed.).  1979.  Proceedings of the Sixth Annual Conference on
        Wetlands  Restoration and Creation.  Hillsborough Community College,
        Tampa, Florida.  Sponsored by the Tampa Port Authority and the Envir-
        onmental  Studies Center at Cockroach Bay, FL.  357 pp.
144. Cole, H., W.  Merrill, F.L. Lukezic, and J.R. Bloom. 1969.  Effects on
        Vegetation of Irrigation with Waste Treatment Effluents and Possible
        Plant Pathogen-irrigation Interactions.  Phytopathology 59:1181-1191.
145.  Cooke, W.B.   1956.   Potential Plant Pathogenic Fungi in Sewage and Pollu-
        ted Water.   Plant Dis.  Rep. 40:681-687.
146.  Cooke, W.B.   1976.   Fungi in Sewage,  p. 389-434.  In:  E.B.G. Jones (ed.)
        Recent Advances  in Aquatic Mycology.  John Wiley & Sons, New York.
147.  Coleman, M.  S., J. P.  Henderson, H. 6. Chichester, and R. L. Carpenter.
        1974.  Aquaculture as a Means to Achieve Effluent Standards,
        pp. 199-215. In Wastewater Use in the Production of Food and Fiber
        Proc. EPA 660/2-74-041.

        This paper presents an advanced biological system based on herbivorus
     or filter-feeding fish for the removal of algae.  This system produces a

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     useful product Ln the form of fish which may be used as bait, restocking,
     or forage.  The Quail Creek Sewage Lagoons in Oklahoma City, OK, were
     used as a large scale test to fish viability.
148. Coler, R. A., and J. Pardo.  1977.  A Test of the Effects of Domestic
        Sewage on the Growth of the Common Blue Mussel, Etytilus gdul. is, in an
        Aquaculture System.  University of Massachusetts, Water Resources
        Center Publication No. 87, 44 pp. (AL)

        The effects of domestic sewage on the growth rate of the marine
     bivalve mollusk, Myjbilus edujJLs was investigated by introduciig con-
     trolled amounts of sewage inbo a raceway system and comparing the
     response with that of organisms in a similar system fed with uncon-
     taminated estuarine water.  The results showed that the admixture of
     domestic sewage with sea water retarded the growth of this organism, and
     indicated that it would not be feasible to use populations of Mytilus to
     reduce the BOD due to sewage sludge in receiving waters. (AL)
149.  Collins, E. R., Jr., E. T. Kornegay, and 0. C. Martens.  1978.  Swine
        Lagoon Effluent on a Soil-Plant Environment:  An Impact Assessment.
        Virginia Water Resources Research Center Bulletin 110.  Virginia
        Polytechnic Inst. and State Univ., Blacksburg, VA.  44 pp.

        Two biological treatment units foe a swine production unit were
     evaluated.  One unit was maintained as an anaerobic system, the other as
     a mixed aerated unit by recirculation of effluent.  Analysis of the
     supernatant of both systems showed that, with time, accumulation of heavy
     metals and other elements increased.  Effluent from both treatment
     systems was irrigated on fescue plots, and effects on soil, soil water,
     and plant tissue were determined.  While no visible deleterious effects
     ware noted over a period of one and one half years, accumulation or high
     uptake of Na, Cu, and Zn was recorded in both plant tissue and soils.
     Nitrate leaching from plots was minimal.  Other waste constituents
     measured did not appear to be limiting factors in land disposal of swine
     lagoon effiuent.  (NT)
150. Colt, J., S. Mitchell, G. Tchobanoglous, and A. Knight.  1979.  The Use
        and Potential of Aquatic Species for Wastewater Treatment.  Appendix
        B: The Environmental Requirements of Fish.  California State Water
        Resources Control Board, Publication No. 65, Sacramento, CA, 240 pp.
151.  Colt, J. et al. 1980a.  The Use and Potential of Aquatic Species for
        Wastewater Treatment.  Appendix C:  The Environmental Requirements of
        Freshwater Crustaceans.  California State Water Resources Control
        Board, Publication No. 65, Sacramento, CA.

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152.  Colt, J.  et al.   1980b.   The Use and Potential of Aquatic Species for
        Wastewater Treatment.   Appendix D:  The Environmental Requirements of
        Freshwater Bivalves.   California State Water Resources Control Board,
        Publication No. 65, Sacramento, CA.
153. Copeland, B. J., and H. L. Davis.  1972.  Estuarine Ecosystems and High
        Temperatures.  North Carolina Water Resources Research Inst. Rept. No.
        72-68.  Raleigh, NC.  103 pp.
154. Copeland, B.J., H.T. Odum, and D.C. Cooper.  1972.  Water Quality for Pre-
        servation of Estuarine Ecology, pp. 107-126.  In: Conflicts in Water
        Resources Planning.  Water Resource Symp. No. 5.  Univ. of Texas,
        Austin.

        Responses of estuarine community structure, respiration and production
     to added heat, sewage and their combination were investigated.  Plastic
     pools containing transplanted ecosystems from South Creek Estuary, NC,
     were used.  Temperature replication was achieved.  Thermal treatment in-
     creased nutrient regeneration rates, yielding slightly higher algal bio-
     mass; although, seasonal differences were more significant.  Sewage addi-
     tion substantially increased ammonia levels, particularly during the win-
     ter.  Community metabolism responded positively to thermal treatment, but
     not to sewage treatment.  Temperature had very little effect on phyto-
     plankton composition during the spring.  A flow-through experiment was
     conducted to test more realistic estuarine conditions.  Results were sub-
     stantially the same as obtained under quiescent conditions.  (NT)


155. Cornwell, D.A., J. Zoltek, Jr., C.D. Patrinely, T. deS. Furman, and J. I.
        Kim.  1977.  Nutrient Removal by Water Hyacinths.  J. Water Pollut.
        Control Fed. 49:57-65.

        Water hyacinths (Eichhornia crassipes) were grown in polishing ponds
     containing secondary effluent from the University of Florida Campus Waste-
     water Treatment Plant.  It was found that water hyacinths had an aera-
     doubling time of about 6 days when growing in secondary effluent compared
     to a 12 day area-doubling time under natural conditions.  Nutrient removal
     was found to be less than 20 percent when the pond depth was 1.4m (4.5 ft)
     and the detention time was 15 h.  Ponds with 0.34 m  (1.1 ft) depth and 48
     h detention time had 80 percent total nitrogen removal and 47 percent
     total phosphorus removal.  A direct correlation was  found to exist between
     the percent nutrient removal and a loading parameter defined as surface
     area per unit flow.  (AA)
156. Costas, P.  1978.  Potomac Estuary Tapped for Pilot Study.  Water and Sew-
        age Works  125(11):64.

        Technical Feature:  An experimental water treatment plant is being con-
     structed to study the feasibility of using the Potomac Estuary as a water

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     supply source.  A water quality analysis program will assay raw, in
     process, and finished waters to evaluate the operating efficiency of
     various treatment processes and to determine the potability of the product
     water.  The site for the plant was selected for its proximity to poor
     quality estuary water and wastewater treatment plant effluent.  The
     estuary receives effluents from several water pollution control plants.
     During severe drought conditions, the percentage of treated wastewater in
     the estuary can be very high.  Consequently, use of the estuary as a
     supplemental water supply source is still only a possibility.  (EL)
157. Coupal, B., and J. Lalancette.  1976.  The Treatment of Wastewaters with
        Peat Moss.  Water Research 10:1071-1076.
158. Crisp, D. T.  1966.  Input and Output of Minerals for an Area of Pennine
        Moorland: the Importance of Precipitation, Drainage, Peat erosion, and
        animals.  J. Appl. Ecol. 3:327-348.
159. Culley, D. D., Jr., and E. A. Epps.  1973.  Use of Duckweed for Waste
        Treatment and Animal Feed.  J. Water Pollut. Control Fed. 45:337-347.
160. Czerwenka, W., and K. Seidel.  1976.  The Combination of Biological and
        Chemical Treatment at the Krefield Water Treatment Works, pp. 287-293
        In; J. Tourbier and R.W. Pierson, Jr. (eds.) Biological Control of
        Water Pollution.  University of Pennsylvania Press, Philadelphia.
161. Damman, A.W.H.  1978.  Distribution and Movement of Elements in Ombro-
        trophic Peat Bogs.  Oikos 30:480-495.
162. Dane, C.W.   1959.  Succession of Aquatic Plants in Small Artificial Marsh-
        es in New York State.  New York Fish and Game Journal 6(10):57-76.

        This report compares studies conducted in 1952, 1953, and 1956 in which
     successional changes in vegetation were measured and evaluated on 22 small
     marshes in the Southern Tier and Genesee-Niagara regions of New York.  The
     latter region was found to be more favorable for abundance of emergent
     cover, abundance of subergent food, retainment of residual woody cover,
     slope of the marsh basin, and marsh-basin depths than the former region.
     Shallower flooding and a greater natural drawdown during the summer months
     permitted the woody cover to remain alive in the marshes of the Genessee-
     Niagara region, but the woody vegetation was largely flooded out in the
     Southern Tier.
        The water level during the growing season was found to be the single
     most important factor in aquatic plant succession in marshes.  Many of the

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     marshes in the Southern Tier had slopes that dropped off sharply to 24
     inches below the normal water level (the limit of regional emergents).
     Overcrowding of emergents occurred frequently in the shallower marshes of
     the Genessee-Niagara region during years with low water level.  (EPA)
163.  Darnell, R., W. E. Pequegrat, B. M. James, F. J. Benson, and R. A.
        Defenbaugh.  1976.  Impacts of Construction Activities in Wetlands of
        the United States.  EPA-600/3-76-045.  U.S. Environmental Protection
        Agency, Corvallis Environmental Research Laboratory, Corvallis, OR,
        393 pp.

        The primary types of construction activity which severely impact wet-
     land environments of the United States include: floodplain surfacing and
     drainage, mining, impoundment, canalization, dredging and channelization,
     and bank and shoreline construction.  Each type of construction activity
     is attended by an identification suite of physical and chemical altera-
     tions of the wetland environment which may extend for many miles from the
     site of construction and may persist for many years.  In turn, each type
     of physical and chemical modification has been shown to induce a derived
     set of biological effects, many of which are predictable, in general, if
     not in specific detail.  The most environmentally damaging effects of con-
     struction activities in wetland areas, in order of importance, are: direct
     habitat loss, addition of suspended solids, and modification of water
     levels and flow regimen.  Major construction-related impacts also derive
     from altered water temperature, pH, nutrient levels, oxygen, carbon
     dioxide, hydrogen sulfide, and certain pollutants such as heavy metals,
     radioactive isotopes, and pesticides.  Over one third of the nation's wet-
     lands have been lost through various forms of direct habitat destruction,
     and well over half of the remainder have been severely modified.  Many
     aquatic species are known to have been lost or severely restricted, and a
     number of species and habitats are currently in jeopardy, at least in part
     as a result of construction activities.  Deliberate and drastic action is
     required to reverse the present trends, and recommendations are given for
     specific steps which must be taken to insure the survival of the wetland
     ecosystems of the nation.  (AA)
164. Davis, A. C.  1980.  Is This the Decade for Aquaculture?  II.  Compost
        Science/Land Utilization 81(2):37-39.

        Some papers presented at a seminar on aquatic systems for purifying
     waste waters held at the University of California, Davis, are  summarized.
     The Arkansas Game and Fish Commission are using silver and bighead  carp
     for water quality improvement.  These fin fish have especially adapted
     gill rakers efficient at filtering particles down to microns.  Benton
     Wastewater Treatment Facility has a system of 6 sewage lagoons in series,
     the last 4 containing fish; BOD and suspended solids have been lowered by
     96 and 86 per cent, respectively, with fish production of 5000 Ib per
     acre year.  Work at Michigan State University on ecological limits  to the
     use of alternative systems of wastewater management is noted,  as is a
     study of energy from wastewater aquaculture systems.  The Denver Research

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     Institute is examining the legal, political, and social implications of
     wastewater treatment through aquaculture.  (AL)
165. Davis, C.B, J.L Baker, A.G. Van der Valk, and C.E. Beer.  1981.  Prairie
        Pothole Marshes as Traps for Nitrogen and Phosphorus in Agricultural
        Runoff, pp.  153-164.  In; B. Richardson (ed.), Selected Proceedings of
        the Midwest  Conference on Wetland Values and Management.  Minnesota
        Water Planning Board.  St. Paul.  660 pp.

        Water quality of drainage influents and the lone effluent at Eagle Lake
     marsh, Iowa, was studied for 4 years (1976-1979).  Because of drought con-
     ditions, there  was no effluent from the marsh in 1976, 1977, or 1978.  In
     1979, the marsh was effective at removing inorganic N, especially NO^-N,
     from runoff water passing through.  It has little impact on levels of
     inorganic-P, total-P, and Kjeldahl-N; it was a net exporter of soluble or-
     ganic carbon.  (AA)
166. Davis, G. J. ,  and M. M. Brinson.  1980.  Responses of Submersed Vascular
        Plant Communities to Environmental Change.   U.S.  Fish and Wildlife
        Service, FWS/OBS-79/33.  70 pp.
167. Davis, H. 1978a.   Effects of the Treated Effluent on Mosquito Populations
        and Arbovirus  Activity, pp. 361-428.  In H.T.  Odum and K.  C.  Ewel
        (eds.).  Cypress Wetlands for Water Management, Recycling and Conser-
        vation,  Fourth Annual Report.  University of Florida, Gainesville.
168. Davis, H. G. 1978b.  Recycling Treated Sewage through Florida Cypress
        Swamps:   Its Effect on Mosquito Population and Arbo-virus Implica-
        tions.  Ph.D. Diss., Department of Entomology, University of Florida,
        Gainesville.  163 pp.
169.  Davis, S.M. ,  and L.A.  Harris, 1978.  Marsh Plant Production and Phosphor-
        us Flux in Everglades Conservation Area 2.   pp. 105-131, In;  Drew, M.A.
        (Editor),  Environmental Quality Through Wetlands Utilization.  A Sym-
        posium on  Freshwater Wetlands, 28 February-2 March 1978.  The Coordi-
        nating Council on the Restoration of the Kissimmee River Valley and
        Taylor Creek-Nubbin Slough Basin, Tallahassee, FL.
170.  Day, J.W.,  Jr., and G.  Paul Kemp.  1983.  Long-term Impacts of Agricultur-
        al Runoff in a Louisiana Swamp Forest (draft manuscript).  In;  Ecologi-
        cal Considerations in Wetlands Treatment of Municipal Wastewaters.  Pro-
        ceedings of a Workshop, 23-25 June 1982, University of Massachusetts,
        Amherst, MA.  US Fish and Wildlife Service and US Environmental Protec-
        tion Agency.

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171.  Deghi, G.A.,  1977.   Effect of Sewage Effluent Application on Phosphorus
        Cycling in Cypress Domes*   Master's Thesis University of Florida,
        Gainesville.   143 pp.
172.  Deghi, G.  S.,  K.  C. Ewel, and W. Mitsch.  1980.  Effects of Sewage
        Effluent Application on Litter Fall and Litter Composition in Cypress
        Swamps. J. of Applied Ecol.  17:397-408.

        No increase in litter fall was observed after two years of addition of
     sewage to  a cypress dome in northern Florida.  The rate of decay of
     cypress needles in litter bags also was not observed to change in the
     presence of the effluent.  The authors concluded that even if litter fall
     were to increase, litter accumulation probably would not increase.  (EPA)
173. DeJong, J.  1976.  The Purification of Wastewater with the Aid of Rush or
        Reed Ponds, pp. 161-172.  In J. Tourbier and R. W. Pierson, Jr.
        (eds.).  Biological Control of Water Pollution.  University of Penn-
        sylvania Press, Philadelphia, PA.

        This research was performed in the Netherlands on artificial marshes
     constructed for the treatment of primary sewage effluent.  Experimental
     treatment of the sewage of a camping site with a rush (Scirpus lacustris)
     pond, one hectare (2.5 acres) in size, began in 1967.  In 1969, a number
     of experimental ditches for sewage treatment were dug that enabled a
     comparison of rushes, reeds (Phragnvites australis), and polypropene
     fibers in purifying sewage.  The aquatic plants functioned mainly by
     providing attachment sites for micro-organisms purifying the sewage and
     also consumed part of the nitrogen and phosphorus supplied to the pond.
     Research has been conducted at this site since 1967, and additional
     studies have been performed at a larger scale facility (a treatment plant
     of 40,000 population equivalents).  (EPA)
174. DeJong, J.  1975.  Bulrushes and Reed Ponds.  Rijksdienst Voor de Ijssel-
        meer-polders.  Flevobricht.  N.R.  104.
175. Demgen, F. C.  1979.  Water Used One More Time: Treated Wastewater for
        Wildlife.  Outdoor California, pp. 28-29.
176. Demgen, F. C.  1979.  Wetlands Creation for Habitat and Treatment - at
        Mt. View Sanitary District, California, pp. 61-73.  In R. K. Bastian
        and S. K. Reed (eds.).  Aquaculture Systems for Wastewater Treatment:
        Seminar Proceedings and Engineering Assessment.  EPA 430/9-80-006.
        U.S. Environmental Protection Agency, Office of Water Program Oper-
        ations, Municipal Construction Division.

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177. Demgen, F. C., and B. J. Blubough. 1977.  Mt. View Sanitary District
        Marsh Enhancement Pilot Program.  Progress report No. 3, Contra Costa
        County, CA, 50 pp.
178. Demgen, F. C., and J. W. Nute.  1977.  Marsh Enhancement Program: Con-
        ceptual Plan for Mt. View Sanitary District, Contra Costa County, CA,
        143 pp.
179. Demgen, F. C., and J. W. Nute.  Wetlands Enhancement Using Secondary
        Effluent.  Presented at the National Conference on Environmental
        Engineering, Research Development and Design.  Kansas City, MO.
        10-12 July 1978.
180. Demgen, F. C., and J. W. Nute.  Wetlands Creation Using Secondary Treated
        Wastewater.  Presented at the AWWA Research Foundation Water Reuse
        Symposium.  Washington, DC.  March 25-30, 1979.

        Technical Feature:  The concept of using treated sewage effluent as a
     freshwater source for the creation and restoration of wetland ecosystems
     of San Francisco Bay is investigated.  The wetlands habitats, including
     vegetation and wildlife, and wetlands restoration facilities are des-
     cribed.  Water quality characteristics of the effluent, such as DO, BOD,
     suspended solids, and nutrients, are surveyed.  The benefits of this
     wetlands system include improved water quality, habitat creation or
     enhancement, and recreational and educational opportunties.  (EL)
181. deMichel, E., and R. M. Trip.  1975.  Estimate of Treatment Plant Efflu-
        ent Impact on Chloride Concentrations and Oyster Environment within
        the Murderkill Estuary.  Water Research 9(12):1107.

        Technical Report:  The effect of treatment plant effluent on chloride
     concentrations and oyster environment within the Murderkill River
     Estuary, DE, is investigated.  Use of a one-dimensional steady state
     model for a conservative substance shows that chloride values would be
     reduced by up to 20% at low water slack periods for wastewater flows of
     10 MGD-1.  Chloride additions in the treatment plant effluent should be
     implemented to maintain minimum summertime low water slack levels of 5.6
     PPT, consistent with a proper environment for oyster development. (EL)
182. DePauw, N. et al.  1976.  Research on the Tertiary Treatment of Swine
        Waste by Mass Culturing of Algae.  International Symposium on "Experi-
        mental Use of Algal Cultures in Limnology", Sandefjord, Norway, Oct.
        26-28, 1976.

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183.  DePauw,  N.  and L.  Deleenheer.   1977.   Mass Culturing of Marine and Fresh-
        water Algae on  Aerated Swine Manure.   Presented at the Conference:
        "Cultivation of Fish Fry and its Live Food", Szymbark, Poland,  Sept.,
        23-28, 1977.
184.  Dewitt, C.B.  and E.  Soloway (eds.), 1978.   Wetlands:  Ecology,  Values, and
        Impacts.   Proceedings of the Waubesa Conference on Wetlands, June 2-5,
        1977.  Madison, WI.   388 pp.

        The 27 papers presented in the proceedings of the  Waubesa Conference on
     Wetlands are  grouped into eight categories:  wetland soils,  nutrition, and
     productivity, environmental impacts, wetland protection and restoration,
     wetlands for  wastewater treatment, wetland system studies,  wetland ani-
     mals, wetland mapping,  and patterns and dynamics of wetland communities.
     The abstracts of seven  papers not presented in this volume  are presented
     in Appendix 1.  A majority of the papers deal with the wetlands of Wis-
     consin.  A list of films on wetlands and related topics, excerpts from the
     conference program and  an index are provided.
185.  Dickson, K.  L., J.  Cairns, Jr., J.  R.  Clark, and J.  H.  Rodgers.   1978.
        Evaluating Pollution Stress on Ecosystems, pp. 80-83.  In K.  C. Flynn,
        and W. T. Mason (eds.).  The Freshwater Potomac - Aquatic Communities
        and Environmental Stresses.  Proceedings of a symposium held in
        January 1977 at College Park, MD.  Interstate Commission on the
        Potomac River Basin, Rockville, MD.
186. Dierberg, F. E., and P.  L. Brezonik.  1976.  Denitrification and Surface
        Runoff in the Experimental Domes, pp. 353-374.  In H. T. Odum, K. C.
        Ewel, J. W. Ordway, and M. K. Johnston (eds.), Cypress Wetlands for
        Water Management, Recycling, and Conservation.  Third Annual Report.
        Center for Wetlands.   Univ. of Florida.  Gainesville.
187. Dierberg, F.E., and P.L. Brezonik.  1978.  The Effects of Secondary Sewage
        Effluent on the Surface Water and Groundwater Quality of Cypress Domes,
        pp. 178-270.  In; H.T. Odum and K.C. Ewel (eds.).  Cypress Wetlands for
        Water Management, Recycling, and Conservation.  Fourth Annual Report to
        the National Science Foundation and the Rockefeller Foundation.  Center
        for Wetlands, University of Florida, Gainesville.
188. Dierberg, F.E., 1980.  The Effects of Secondary Sewage Effluent on the
        Water Quality, Nutrient Cycles and Mass Balances, and Accumulation of
        Soil Organic Matter in Cypress Domes.  Ph.D. Dissertation.  University
        of Florida, Gainesville.  3/24 pp.

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        The capability of cypress domes to act as efficient nutrient  traps  has
     been demonstrated for a natural and sewage-enriched cypress dome.  Mass
     balance models indicated that 87% of the nitrogen and 92% of the phosphor-
     us loadings were retained within a cypress dome receiving secondary  sewage
     effluent, removal efficiencies that are among the highest reported for any
     wetland ecosystem receiving treated sewage.  Thus, cypress domes seem  to
     be effective natural tertiary treatment systems.  Field data and labora-
     tory investigations demonstrated that denitrification was a major nitro-
     gen sink in both natural and sewage-enriched domes.  Bulk precipitation
     was the most important factor in supplying new minerals and nutrients
     (except nitrogen) to the natural cypress dome.  Analyses of standing
     waters of natural and sewage-enriched cypress domes near Gainesville,
     Florida, over a 4.5 year period showed that discharge of secondary sewage
     effluent altered the soft, acid water found in natural domes to  a neutral,
     moderately hard condition.  Furthermore, the absence of dissolved oxygen,
     high levels of phosphorus, nitrogen, biochemical oxygen demand,  and  the
     presence of hydrogen sulfide mean that treated sewage effluent has a sub-
     stantial impact on water quality.  Water samples from shallow wells, cer-
     amic soil moisture tubes, and laboratory percolation columns indicated
     that the underlying organic matter and sands were serving as an  effective
     barrier to the transport of sewage pollutants to the shallow aquifer imme-
     diately below.
189. Dinges, R. 1973.  Ecology of Daphnia. in Stabilization Ponds.  Texas State
        Department of Health Resources, Austin, TX., pp. 133-136.

        The objectives of this report were: to locate Texas wastewater stabil-
     ization ponds supporting Daphnia populations and identify those environ-
     mental factors which are favorable, or inimical to Daphnia, and to use in-
     formation acquired in field studies of "Daphnia. Ponds" to establish a
     pilot pond for maintenance of a mass paphnia culture to use in clarifica-
     tion of stabilization pond effluent.  (NT)
190. Dinges, R.  1974.  The Availability of Daphnia for Water Quality Improve-
        ment and as Animal Food Source, pp. 142-161.  In Wastewater Use in the
        Production of Food and Fiber-Proc.  U. S. Environmental Protection
        Agency. Ada, OK.
191.  Dinges, R. 1976a.  A Proposed Integrated Biological Wastewater Treatment
        System, pp. 225-230.  In J. Tourbier and R. W. Pierson, Jr. (ed,).
        Biological Control of Water Pollution.  University of Pennsylvania
        Press, Philadelphia, PA.

        Construction of an experimental facility at an area wastewater treatment
     plant is underway to evaluate potential employment of various animals and
     plants in an integrated, sequenced system for improvement of water  quality.
     The wastewater treatment plant consists of an aeration basin, clarifier, and
     three stabilization ponds operated in series.  Input to the experimental
     unit will be a portion of the effluent from the terminal stabilization pond.

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     Stabilization pond effluent in which original organic material has been re-
     duced by bacterial action and converted into algae cells would be of high
     quality except for algae present and high bacteria and nutrient levels. The
     study scheduled to commence in the near future will incorporate information
     gained from past studies and include several new concepts.  It is intended
     to correct the deficiency of stabilization pond effluent quality.
192. Dinges, R. 1976b.  Water Hyacinth Culture for Wastewater Treatment.  Texas
        Department of Health, Division of Wastewater Technology and Surveil-
        lance, Austin, TX.  143 pp.
193. Dinges, R. 1976c.  Who Says Sewage Plants have to be Ugly?  Water and
        Wastes Engineering  13(4):20-23.
194. Dinges. R. 1978.  Upgrading Stabilization Pond Effluent by Water Hyacinth
        Culture.  J.  Water Pollut. Control Fed.  50(5):833-845.

        Water hyacinths (Eichhprnia crassipes) were grown in a series of four
     earthen basins provided with stabilization pond effluent to determine the
     effect of their cultilvation on water quality.  System effluent contained
     less than 10 ing/1 of suspended solids of 5-day biochemical oxygen demand.
     Significant reductions in fecal bacteria and nitrogen were recorded. Most
     of the improvement in quality obtained may be attributed to the unique
     habitat afforded by a dense stand of hyacinths.  Mineral nutrients and
     heavy metals were accumulated by the plants during active growth.  Basic
     culture basin design considerations and the potential applications of hya-
     cinth treatment are discussed.  (AA)
195. Dinges, R.  1979.  Development of Hyacinth Wastewater Treatment Systems
        in Texas, pp. 193-226.  In R. K. Bastian and S. C. Reed  (eds.).
        Aquaculture Systems for Wastewater Treatment: Seminar Proceedings and
        Engineering Assessment.  U.S. Environmental Protection Agency.  Office
        of Water Program Operations, Municipal Construction Division.  EPA
        430/9-80-006.

        There is sufficient information available for designing  hyacinth treat-
     ment facilities to be employed in warm climates.  The optimal design of
     culture basins is necessary to minimize areal requirements  and greenhouse
     costs in temperate climates.  Hydraulic loading is the most critical con-
     sideration in culture basin design.  The primary pollutant  removal mechan-
     ism of hyacinth treatment is the reduction of suspended particulate
     content.  The harvesting of hyacinths disrupts treatment.   Provision of
     multiple culture basins to allow alternate operation is desirable.  Each
     culture basin should be drained and the accumulated sludge  and plant
     debris removed on an annual basis.  Hyacinth culture may be employed as a
     complete treatment process.  Nitrogen management is a key factor  in util-
     izing hyacinths for wastewater treatment, or for biomass production.   Sta-

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     bilization ponds can be designed for effective nitrogen reduction.  Hya-
     cinth culture will remove most remaining nitrogen in pond effluent, espe-
     cially that which is in the organic form.  Stabilization ponds  followed by
     hyacinth culture constitutes a highly effective wastewater treatment
     system.  A hyacinth treatment system is capable of producing effluent
     having a mean content of <10mg/l 8005 and TSS.  (AA)
196.  D'ltri, F.M. (ed.).  1977.  Wastewater Renovation and Reuse.  Marcel
        Dekker, Inc., New York.
197. D'ltri, F.M. (ed.). 1981.  Vegetation Management for Land Treatment of
        Municipal Wastewater.  Great Lakes National Program Office U.S.
        Environmental Protection Agency.  Chicago, IL.  163 pp.

        A conference/workshop was held at Michigan State University on Febru-
     ary 23-25, 1981, to review, discuss, and recommend vegetation management
     strategies and cultivars which can be used most effectively in slow rate
     and overland flow municipal land treatment systems in the north central
     region of the United States.  Eleven research papers were presented on
     various aspects of vegetation selection and management such as (a) irri-
     gation system design, (b) wastewater crop management, (c) vegetation
     selection and management for overland flow systems, (d) tree and old field
     responses to wastewater irrigation, and (e) plant diseases associated with
     municipal wastewater irrigation.  The participants and audience for the
     conference consisted of scientists, wastewater treatment plant operators,
     and state and local governmental officials.
        The workshop phase was limited to the speakers and Environmental Pro-
     tection Agency representatives.  They evaluated more fully the information
     presented during the conference to summarize the current state of the art
     and identify future research needs.
198.  Dixon, K.  R.,  and J. A. Kadlec.  1975.  A Model for Predicting the
        Effects of  Sewage Effluent on Wetland Ecosystems.  Wetlands Ecosystem
        Research Group.  Univ. of Michigan, Ann Arbor.

        This report examines a mathematical model of the organic matter com-
     ponents of a wetland ecosystem—its objective, its development, and its
     analysis.   The objective of the model is to be able to forecast the
     effects of sewage wastewater disposal on the ecosystem; this requires
     that the variables of water and nutrients be included in the models of
     the several processes which may be affected by the wastewater disposal.
     The model  consists of a set of ordinary first-order, nonlinear differ-
     ential equations representing the mass balance of the biomass components
     of the system.  Its analysis indicates the model's credibility as a
     first-time model that can be used as an aid in making decisions about the
     feasibility of using a wetland ecosystem for wastewater disposal.  (NT)

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199. Dodge, D. E.   1972.  Avian Botulism: Factors Affecting its Occurrence on
        Domestic Sewage Lagoons.  Master's Thesis, Utah State University,
        Logan.  101 pp.

        The sewage lagoons for the city of Logan, Utah, were investigated for
     factors which are conducive to the death of ducks and other water birds
     by botulism.   Observations on waterfowl populations in relation to
     botulism were conducted during the summer and fall of 1970 and 1971.
     Ecological features of the lagoons were also studied to determine if
     outbreaks were related to ecological phenomena.
        During 1970 approximately 140 birds died from botulism between August
     14 and September 30.  In 1971 35 birds died between August 11 and
     September 15.  Data for 1969 showed that approximately 2000 birds died
     between July and September of that year.  Botulism losses all 3 years
     correlatd with peaks in the invertebrate populations, especially chirono-
     mid larvae, in some of the ponds and with large blooms of the blue-green
     algal Microcystis aeruginpsa.  The decreasing severity of the botulism
     outbreaks from 1969 to 1971 was probably the result of several factors.
     The abundance of chironomids in the sediment of lagoons appeared to
     decline from 1969 to 1971, the toxigenicity of the botulinum bacteria in
     the lagoons may have been reduced from 1969 to 1971 , and the number of
     decaying bird carcasses left on the lagoon ponds was less in 1970 and 1971
     than in 1969.  (AA)
200. Dolan, T.  1978.  Effect of Sewage Effluent on the Phosphorus Budget of a
        Marsh.  Master's Thesis.  Department of Environmental Engineering
        Sciences.  University of Florida, Gainesville.  77 pp.
201. Dolan, T.J., S.E. Bayley, J. Zoltek, Jr., and A. Hermann.  1978.  The
        Clermont Project:  Renovation of Treated Effluent by a Freshwater
        Marsh, pp. 132-152.  In M. A. Drew (ed.), Environmental Quality
        through Wetlands Utilization.  Proceedings of a symposium held 28
        February-2 March 1978, at Tallahassee, FL.  sponsored by the Coor-
        dinating Council on the Restoraton of the Kissimmee River Valley and
        Taylor Creek-Nubbin Slough Basin.  Tallahassee, FL.
202. Dolan, T.J., S.E. Bayley, J. Zoltek, Jr., and A.J. Hermann.   1981.  Phos-
        phorus Dynamics of a Florida Freshwater Marsh Receiving Treated Waste-
        water.  Journal of App. Ecol. 18(1):205-219.

        Phosphorus in secondary effluent, applied weekly for a year to a test
     plot, was over 97% assimilated.  Soil  (peat, clay, and sand) was the major
     sink, with secondary sinks in the below-ground biomass and litter.  Three
     2000 sq. meter plots received effluent at the rates of 1.3, 3.8, and 10.2
     cm per week, with a control plot receiving 3.8 cm per week of freshwater.
     Vegetation present included Sag^ttaria landfolia, Pontederia cordata,
     Panicum spjo. and Hibiscus sp_.  The plot with the highest rate of effluent
     loading showed increases in net production of plant shoots, litter, root
     and rhizomes, and in P concentration in living and dead plant tissue,

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     compared with the control plot.  There was 38.03 g P per  sq  meter applied
     to the high effluent plot during the course of the study;  26.31,  8.81,  and
     1.97 g P per sq meter were stored in soil, roots and rhizomes,  and litter,
     respectively.  Outflow from the plot was 0.94 g P per  sq  meter.   The
     freshwater control plot received a total of 0.38 g P per  sq  meter, stored
     0.21 g P per sq meter in the litter, and lost 0.17 g P per sq meter in  the
     outflow.  There was no evidence of a spring flush of P from  any plot.
203. Dollar, S. J.  1979.  Ecological Response to Relaxation of Sewage Stress
        Off Sand Island, Oahu, HI.  Kept. No. 124.  Univ. of Hawaii, Oahu.
204. Donaszy, E.  1974.  Principles of Sewage Treatment through Utilization  in
        Fish Ponds, pp. 546-547.  In Wastewater Use in the Production of  Food
        and Fiber-Proc.  U. S. Environmental Protection Agency.  Ada, OK.
205. Dovovan, J.F. and J.E. Bates, 1980.  Guidelines for Water Reuse.  EPA
        Report No. EPA-600/8-80-036.  August.  115 p.

        In order to increase interest in and assist implementation of waste-
     water reuse for nonpotable purposes, guidelines have been developed to
     make water managers and resource planners aware of proven reuse possi-
     bilities.  Perceived benefits in water reuse include: conservation of
     water; recycling of nutrients; practical cost and energy savings; reduc-
     tion in the discharge of pollutants; realization of other public priori-
     ties such as preservation of open space for aesthetic and recreation
     value; and encouragement of industrial recycling.  Following a step-by-
     step approach provided in the guidelines, the water manager and resource
     planner can address the principle areas of concern in water reuse pro-
     grams , including technology, economics, legal issues, institutional
     arrangements, markets, and public information.  Case histories provide in-
     sight into actual reuse experience.  Potential markets for reclaimed water
     include: groundwater recharge; recreation; environmental enhancement.
206. Dorris, T. C., S. L. Burks, and G. R. Waller.  1974.  Effects of Residual
        Toxins in Oil Refinery Effluents on Aquatic Organisms.  Technical
        Completion Report, Oklahoma Water Resources Research Inst. , Still-
        water, OK.  81 pp.

        Laboratory evaluations of partial evaporation of oil refinery waste-
     waters were performed to determine removal of toxic substances by partial
     evaporation, suitability of discharge to a receiving stream of the bottom
     partially concentrated waters from an evaporator, and acceptability of
     overhead evaporator product water for reuse as boiler make-up or other
     uses.   The wastewaters from biological treatment—lagoon and activated
     sludge units-and from primary treatment facilities—API separator and air
     flotation—were partially evaporated in rotating thin-film or columnar
     falling film evaporators.  Conclusions were that partial evaporation of

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     biological treated oil refinery wastewater, removes toxic substances from
     biologically treated oil refinery wastewaters, produces an overhead
     product water which has low inorganic salt concentration and should be
     reusable for boiler make-up or other uses where high-quality water is
     desirable, and produces a partially concentrated bottom product water
     generally less toxic. (NT)
207. Dressier, R. L., and G. W. Wood.  1976.  Deer Habitat Response to Irriga-
        tion with Municipal Wastewater.  J. Wildl. Manage. 40(4):639-644.

        The influence of wastewater irrigation on the quantity and quality of
     forage production and white-tailed deer (Odocoileus yIrffinianus) feeding
     response was studied in central Pennsylvania.  Little difference was found
     in the amounts of available forage during the early spring months. During
     the growing season the production of palatable forage on the irrigated
     site was considerably less than on the non-irrigated site.  Concentrations
     of crude protein, P, K, and Mg in forage generally were increased by irri-
     gation as was present digestible dry matter.  Observations of semi-free
     ranging lead deer did not indicate any deterrence from use of the site due
     to wastewater irrigation.  (AA)
208. Drew, M. L.  1975.  Decomposition of Cypress Dome Litterfall by Fungal
        Populations.  Master's Thesis, Department of Environmental Engineering
        Sciences, Univ. of Florida, Gainesville.  75 pp.
209. Drew, J. A.  (ed.).  1978.  Environmental Quality through Wetland
        Utilization. Proceedings of a symposium held 8 February-2 March 1978,
        at Tallahassee, FL.  Sponsored by the Coordinating Council on the
        Restoration of the Kissimmee River Valley and Taylor Creek-Nubbin
        Slough Basin.  Tallahassee, FL.  743 pp.
210. Drifmeyer, J.E. , and W. Odum.  1975.  Lead, Zinc, and Manganese in
        Dredge-Spoil Pond Ecosystems in Virginia.  Environ. Conserv. 2:1-7.
211. Duever, M.J. , J.E. Carlson, L.A. Riopelle, and L.C. Duever.  1977.  Eco-
        system Analysis at Corkscrew Swamp.  Pages 534-570.  In; H.T. Odum and
        K.C. Ewel (eds.), Cypress Wetlands for Water Management, Recycling and
        Conservation.  4th Annual Report to the National Science Foundation and
        the Rockefeller Foundation, Center for Wetlands, University of Florida,
        Gainesville.
212. Duffer, W. R., and C. C. Harlin.  1979.  Potential of Aquaculture for
        Reclamation of Municipal Wastewater, pp. 740-747.  In AWWA Research
        Foundation, Proceedings of the Water Reuse Symposium, Vol. I.

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        Survey Report:  Wastewater aquaculture is surveyed.  Federal R & D
     programs concerning wastewater reuse in aquaculture facilities are sum-
     marized.  Production aquaculture is differentiated from treatment process
     aquaculture.  The latter emphasizes the culture of organisms for waste-
     water treatment and management, and the former emphasizes production.
     Research needs concern aquatic macrophytes, wetlands, food chain pro-
     cesses, economic evaluations, finfish, and invertebrates.
213. Duffer, W. R., and J. E. Hoyer.  1978.  Municipal Wastewater Aquaculture.
        EPA-600/2-78-110.  U.S. Environmental Protection Agency, Wastewater
        Management Branch, Robert S. Kerr Environmental Research Laboratory,
        Ada, OK.  47 pp.

        The developmental status of the aquacultural alternative for treatment
     and reuse of municipal wastewater was reviewed.  Major emphasis was  given
     to the reduction or fate of pollutants in such areas as organics,  solids,
     nutrients, heavy metals, residual hydrocarbons, and potentially path-
     ogenic organisms.  Economic assessments of treatment and production  rates
     for organisms were included for several types of aquacultural processes.
     wetlands, macrophytes, invertebrates, fish and integrated or food  chain
     units.  Based on the literature examined, the information available  at
     this time is not adequate for the design of operational aquaculture
     systems to treat or utilize inputs of municipal wastewater.  Sufficient
     exploratory studies have been conducted, however, to demonstrate a
     definite potential for development of wastewater aquaculture systems.
     Areas having high potential for further research and development were
     identified.  This report covers recent research progress in municipal
     wastewater aquaculture as of June 1977. (AA)
214. Dunigan, E. P., R. A. Phelan, and Z. H. Shamsuddin.   1975.  Use of Water
        Hyacinths to Remove Nitrogen and Phosphorus from Eutrophic Waters.
        Hyacinth Control J. 13:59-61.
215. Dunstan, W.M., and K.R. Tenore.  1972.  Intensive Outdoor Culture of
        Marine Phytoplankton Enriched with Treated Sewage Effluent.  Aquacul-
        ture 1: 181-192.

        The authors describe studies on the development of a combined tertiary
     sewage treatment and aquaculture system in which the effluent from sec-
     ondary sewage treatment is used as a growth medium for unicellular marine
     algae, and the algae, in turn, are used as food for bivalve molluscs.
     Under outdoor conditions, the sewage enrichment of sea water stimulated
     the growth of a variety of micro-algae which were usually dominant in the
     water before enrichment.  Several sources and samples of sewage effluent
     were successfully used for enrichment.  (AL)

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216. Dunstan, W.M. and K.R. Tenore, 1974.  Control of Species Composition in
        Enriched Mass Cultures of Natural Phytoplankton Populations.  J. Appl.
        Ecol. 11(2):529-536.

        The changes in species composition of intensively harvested outdoor
     cultures of enriched natural populations of micro-algae were studied using
     a variety of nutrient additions and dilution rates.  The control of
     species in such semi-continuous outdoor cultures is not only important for
     aquaculture systems but is also helpful in identifying the effects of
     eutrophication on coastal phytoplankton populations.  In a series of 3
     experiments in which cells were counted and identified during the 6th-10th
     day of the'daily 50% harvest, N, P and SI definitely favored the growth of
     diatoms.  The addition of just N and P or N, P and trace metals stimulated
     a green microalgae population.  Addition of treated sewage effluent
     resulted in a 70-80% diatom culture.  In another series of experiments the
     rate of daily dilution was varied and in 2 experiments in June a clear
     control of species composition resulted.  In 10% daily dilution green
     micro-algae dominated while at 75% dilution diatoms dominated particularly
     Skeletqnema costatum (now Stephanopyxis costata).  The results from exper-
     iments in July and August were not as definitive as those in June and the
     ubiquitous diatom Cylindrptheca clqsterium prevailed at all dilution
     rates.
217. Dunstan, W. M., and H. L. Windom.  1975.  The Influence of Environmental
        Changes in Heavy Metal Concentrations of S^artina. alternifIgra,
        pp. 393-404.  In L. E. Cronin (ed.), Estuarine Research, Vol. II,
        Geology and Engineering.  Academic Press, NY.
218. Dworkin, J. M.  1978.  Wastewater Management and Decision-Making:  The
        Role of Community Acceptance in the Adoption of Land Treatment.  Ph.D.
        Diss., Dept. of Geography, Clark University, Worchester, MA.  246 pp.
219. Dykyjova', D. and J. Kvet (ed.).  Pond Littoral Ecosystems:  Structure and
        Functioning.  Springer-Verlag, New York.  1978.

        The book is the result of the Czechoslovak IBP wetlands project which
     focused on intense quantitative ecological investigations of shallow
     littoral ecosystems of typical Central European fishponds.  The book is
     divided into several sections: (i) General ecology and inventorization of
     biotic communities, (ii) Environmental factors in fish pond littoral,
     (iii) Primary production and production processes in littoral  communities,
     (iv) Structure and functioning of algal communities in fish ponds,  (v) De-
     composition processes in the fish pond littoral, (vi) Structure and role
     of animal populations in fish pond littoral, (vii) Effect of fish pond
     management on the littoral communities, and  (viii) Conservation of plant
     communities and waterfowl in wetlands of Czechoslovakia.  The  largest sec-
     tion was section 3 on primary production.  Generally, the book is a com-
     pilation of data generated by the various studies with little  transition
     from one section to another and, although one section will refer to

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     another, the editors made very little effort to  tie  the material  together.
     As stated by the editors "the volume does not present a final  synthesis  in
     the form of overall ecosystems budgets and models/ but the  editors  have
     attempted to give as much as possible for condensed  quantitative  data  as
     is needed for syntheses and ecosystem modeling."  This book would probably
     be a good reference work for a library, but individual researchers  should
     examine a copy before ordering one for their personal reference.
220. EEC Company.  1979.  The Marsh-Forest System:  A Pleasant and Positive
        Answer for Water Reclamation.
221. Ecology Consultants.  1977.  Reclamation for Wildlife Habitat.   In;  Pro-
        ceedings of the Reclamation Workshop, Ft. Colllins, CO.
222. Eckenfelder, W. W., and M. P. Wanielista.   1978.  Advances  in Water  and
        Wastewater Treatment.  Biological Nutrient Removal.  Ann Arbor  Science
        Publishers, Inc. Ann Arbor, MI.  286 pp.

        The present status of biological nutrient removal  systems is  reviewed.
     Kinetics of relevant biochemical reactions  and the design and operation
     of a number of different practical treatment systems  and equipment are
     discussed.  The roles of water hyacinths and of cypress swamps in  de-
     creasing or eliminating the nutrient load are considered.   A separate
     chapter is devoted to the operation of biological fluidized beds for
     nitrogen control.  (AL)
223. Edeline, F., and G. Lambert.  1974.  A Simple Simulation Method  for River
        Self-purification Studies.  Water Res. 8(5):297-306.
224. Edds, G.T. and J.M. Davidson.  1981.  Sewage Sludge Viral and Pathogenic
        Agents in Soil-Plant-Animal Systems.  U.S. Environmental Protection
        Agency.  Pub. No. EPA-600/51-81-026.  Health Effects Research Lab.
        Cincinnati, OH.

        In this study, a multi-disciplinary approach was used to determine the
     ultimate fate of various toxic elements or pathogens associated with Flor-
     ida and Chicago municipal sludges when applied to soil-plant-water  systems
     as an alternative method for the utilization of recycled digested munici-
     pal sludge.  Determination was made of the physiologic, pathologic,
     growth, and reproductive responses of cattle, swine, and poultry that were
     fed sludges, grains or forages from soils pretreated with urban liquid  di-
     gested sludges, as well as health effects in mice receiving liver or kid-
     ney tissues from steers and swine exposed to such feeds or contaminants.
        There were minimal differences in growth performance or egg production
     in cattle, swine, or poultry fed forage or grain from soils pretreated

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     with a variety of urban sewage sludges.  Cattle and swine tissues, when
     fed to mice, resulted in alterations of the normal mineral balance as well
     as reproductive performance.  Tissues from animals intended for human con-
     sumption exposed to sarcocyst contaminated sewage sludges may  serve as
     health hazards for animals and humans.
        Application of urban sludges at 19.8 ton/hectare produced equivalent
     plant growth stimulation for corn, barley, wheat, and sorghum  as  commer-
     cial fertilizers.  Certain bacteria, commonly associated with  sludges,
     disappear in a few days after soil or plant application.  However, certain
     viruses and parasites were shown to persist.  New and improved methods
     were developed to monitor persistence as well as assay for the presence of
     drugs or other hazardous materials.
225. Eisenberg, D. M., J. Benemann, and J. Weissman.  Recent Advances  in  the
        Utilization of High Rate Photosynthetic Wastewater Treatment Systems:
        Potential for Reclamation of Municipal and Agricultural Tastewater.
        Presented at the Water Reuse Symposium II.  Washington, DC.  Aug.
        23-29, 1981.

        Wastewater stabilization ponds have  long been recognized  as a  low-cost
     alternative for the treatment of many types of wastewater.   The uncon-
     trolled environment within ponds of conventional design, however, has
     often resulted in unreliable performance.  This and the difficulty of
     removal of algal suspended solids from  the pond effluents have caused
     engineers to no longer design this type of system, and many  existing
     systems have been abandoned in favor of conventional wastewater treatment
     plants which are most costly and more energy intensive.
        With the recent development, at the  University of California Sanitary
     Engineering Research Laboratory, of the paddlewheel mixed high rate
     stabilization pond, an intermediate alternative has become available.
     These ponds provide a degree of control which cannot be achieved  in
     conventional ponds with only a small increase in cost and energy  con-
     sumption.  They make use of intensive photosynthetic oxygenation  and high
     algal productivity to produce high quality reclaimed water and algal-bac-
     terial floes which are of relatively large size and thus can be readily
     removed.
        In this paper the principals of operation will be described as well as
     the details of contruction of the experimental ponds at University of
     California.  Data on wastewater treatment and algal productivity  will be
     presented from two years of continuous  pond operation.  The  implications
     of this data will be discussed with emphasis on the effectiveness of
     treating water for reclamation purposes*
        Two possible applications outside of the municipal wastewater  treat-
     ment field will be discussed.  These are the reclamation of  agricultural
     drainage water from California's Imperial Valley and the on-site  recla-
     mation of wastewater generated in daily, poultry, and livestock oper-
     ations.  In addition to water reclamation both of these applications have
     enormous potential for generation of valuable by-products ranging from
     methane gas or electricity to expensive aquaculture products for  human
     consumption.  (AA)

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226. Engler, R.M., D.A. Antie and W.H. Patrick, Jr.   1976.  Effect  of Dissolved
        Oxygen and Redox Potential and Nitrate Removal in Flooded Swamp  and
        Marsh Soils.  J. Environ. Qual. 5(3):230-235.

        Flooded and marsh soils in the interior swamp and coastal areas  of
     Louisiana have a high capacity for removing 1*03.  Nitrate  added either
     to the shallow floodwater or mixed in the soil rapidly disappeared  under
     laboratory conditions.  Nitrate loss does not take place in the flood-
     water, but is dependent on the NOj moving downward into the anaerobic
     soil layer.  The N(>3 removal capacity is much greater than is  required
     to handle any amount of N©3 -N that is  likely to be present in these
     areas, either from oxidation or ammonium, that has been released from
     organic matter or from NC>3 derived from local runoff from  adjacent
     agricultural areas.  Nitrate loss does  not appear to be inhibited by the
     presence of a small amount of molecular 02 in flooded soils.
227. Engler, R. M., and W. H. Patrick, Jr.  1974.  Nitrate Removal from
        Floodwater Overlying Flooded Soils and Sediments.  J. Environ. Qual.
        34:409-415.

        The floodwater nitrate removal rate of intermittently flooded fresh
     swamp soils and continuously flooded saline marine soils in southern
     Louisiana is quantitatively characterized.  Of the two areas studied, the
     marsh area is the more effective sink for nitrate contaminated waters.
228. Enns, W. R.  1967.  Insects Associated with Midwestern Oxidation Lagoons.
        Terminal Progress Report.  Univ. of Missouri, Columbia.  21 pp.

        The aim of this project was to study the entomological fauna of  sewage
     lagoons.  Fauna studied included insects, Crustacea, mites, spiders,  and
     sowbugs.  Studies were conducted in different types of lagoons from the
     newest, rawest ones to old ones; these were both primary and  secondary
     lagoons.  Other areas of investigation included; predator prey species in
     the lagoons and determination of predators most effective in  supressing
     midges and mosquitoes; presence of insect pathogens and whether they  could
     be manipulated artificially; and the possibility of manipulating insect
     populations to maintain a balanced lagoon.
229. Epstein, L., K. Ditz, and G.R. Safir. 1982.  Plant Disease in an Old Field
        Ecosystem Irrigated with Municipal Waste Water.  J. of Environ. Qual.
        11:65-68.

        During 1978 and 1979, plant disease on foliage was assessed  in an old
     field dominated by goldenrod (Solidago canjidensis and S_. gramini folia) and
     quackgrass (Agrogyron repens) and irrigated with waste water.   Plant path-
     ogens abundant in waste-water-irrigated areas included, Coleosporium
     asterum, Erysiphe cichoracearum, PhyJLlachora gramini s, and Helminthospor-
     ium sp.; such fungi reportedly alternate their life cycles or reproduce on
     economically important plant species.  The increased foliar disease in the
     irrigated areas was probably due to increased moisture.  Frequencies of

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     plant pathogens in the waste water were apparently low since wounded seed-
     lings of numerous crop varieties immersed in waste water remained healthy.
     Alternaria alternata and Stemphylium sarcinaeforine survived equally well
     after 24 days in filter-sterilized waste water or tapwater; however, Er-
     winia hjerbicola and E. atrqsepjtica, survived longer than 24 hours in buf-
     fered tapwater.  (AA)
230.  Epstein, L. and G.R. Safir.  1981.  Plant Diseases Associated with Muni-
        cipal Wastewater Irrigation,  pp. 157-161.  Paper presented at the Con-
        ference on Vegetation Management for Municipal Wastewater Land Applica-
        tion Systems:  Great Lakes Region of the United States, held at Michi-
        gan State University, East Lansing, MI, 23-24 February 1981, prelimin-
        ary draft of the proceedings.  Kellogg Center for Continuing Education,
        Michigan State University, East Lansing, MI, 15 p.

        Over a 24 day period, 0.1 ml aliquots of wastewater or tap water were
     plated onto potato dextrose agar in order to monitor populations.  No dif-
     ferences in the survival of the spores in tap and wastewater were noted.
     It is believed plant parasites and saprophytes compete for nutrients in
     exudates found on plant surfaces.  To examine this interaction, we moni-
     tored the short term survival of a phytopathogenic bacterium, Erwinia
     herbicqla, in wastewater and tap water.  A sterile dilute phosphate buffer
     was added to filter-sterilized wastewater or tap water with or without 0.1
     percent yeast extract (to simulate plant exudate).  After inoculation with
     a uniform bacterial suspension containing approximately 105 cells/ml,
     the flasks were shaken for 24 hr.  Populations at 0 and 24 hr after inocu-
     lation were determined with a dilution series of the liquid on yeast ex-
     tract agar. Both the pathogenic and the saprophytic Erwinia species sur-
     vived better in the wastewater than in the tap water, but grew more rapid-
     ly in the simulated leaf exudate in tap water than in simulated leaf
     exudate in wastewater.
231. Erlich, S.  1966.  Two Experiments in Biological Clarification of Stabili-
        zation Pond Effluents.  Hydrobiologia 27:70-80.
232. Estrada, M., I. Valiela, and J. M. Teal.  1974.  Concentration  and Dis-
        tribution of Chlorophyll in Fertilized Plots in a Massachusetts Salt
        Marsh.  Sea Grant Kept. No. 74-03.  Center for Wetland Resources,
        Louisiana State Univ., Baton Rouge.  30 pp.
233. Evans, D. W., and J. P. Giesy.  1978.  Trace Metal Concentrations  in  a
        Stream-Swamp System Receiving Coal Asn Effluent, pp. 782-791.   In  Pro-
        ceedings  of the Intl. Congress  for Energy and Ecosystem Ecol. and  Coal
        Resource  Devel. Conf. Vol. 2.   Grand Forks.

        Water, suspended solids, and emergent plants were analyzed  for  trace
     metal concentrations in a stream-swamp ecosystem receiving effluents  from

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     a coal ash disposal basin in South Carolina.  Samples were  analyzed  for
     cadmium, cobalt, chromium, copper, iron, manganese, molybdenum,  nickel,
     lead, and zinc.  Results from the polluted stream are compared with  those
     from a stream not receiving ash effluents.  The effluent-receiving stream
     showed small elevations in metal levels in biotic and abiotic components
     compared with the control stream.  (EL)
234. Everest, J.W. and D.E. Davis.  1979.  Studies of Phosphorus Movement Using
        Salt Marsh Microecosystems.  J. Environ. Qual. 8(4):465.

        Microecosystems of a salt marsh community were designed, constructed,
     and used to follow phosphorus flux through the physical and biological
     components of that community.  The biological components  in these  micro-
     ecosystems were smooth cordgrass  (Spartina alterniflora Loisel), diatoms,
     mud fiddler crabs (Upa pugnax Smith), periwinkle snails (Littorina irro-
     rata Say), and ribbed mussels (Geukensia demissa Oiliwyn; formerly Mpdio-
     IUJB demissus Dillwyn).  The physical components were the  marsh soil and
     the seawater.  Phosphorus (P) placed 17.5 cm below the soil surface was
     abundantly present in the smooth  cordgrass leaves and on  the  soil  surface
     4 days later.  No P was secreted  by the leaves of smooth  cordgrass during
     the experiment.  The rapid transfer of phosphorus to the  soil surface was
     found to be the result of the burrowing activity of the fiddler crab. (AA)
235. Ewel, K.C., 1976a.  Changes in Understory Vegetation  in Cypress Domes
        Brought About By Fire and Addition of Sewage,  pp.  171-189.  In;  Third
        Annual Report on Cypress Wetlands, University of Florida,  Center  for
        Wetlands, Gainesville.

        The effects of application of groundwater and sewage on  ecological
     relationships in burned and unburned cypress domes was monitored  over  a
     two-year period since the initial treatments.  There  were rapid changes  in
     dominance and invader species of understory vegetation for  the burned,
     sewage dome than in any of the others. The duckweed layer is  also thicker.
     The presence of sewage does not seem to have had an overriding influence
     in determining vegetation; however, the two sewage domes are  less similar
     to one another in species composition than are the two burned domes.   The
     similarity index for the species composition shortly  after  sewage intro-
     duction and one year later was 78 (possible maximum of 86)  for the
     unburned dome, but only 62 for the burned dome, suggesting  greater con-
     stancy for the flora in the second sewage dome in response  to the stress
     of sewage.  This suggests that the presence of nutrient vegetation at  the
     time of sewage introduction has damped the kinds of changes in the unburn-
     ed dome.
236. Ewel, K. C.  1976b. Effects of Sewage Effluent on Ecosystem Dynamics  in
        Cypress Domes,  pp. 169-196.  Iia D. C. Tilton, R. H. Kadlec,  and C.  J.
        Richardson (eds.), Proceedings of A National Symposium on Freshwater
        Wetlands and Sewage Effluent Disposal.  Univ. of Michigan, Ann Arbor.

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        Studies of experimental treatment of cypress domes in Florida with
     wastewater are summarized.  A dramatic seasonal increase was noted for
     three species of floating plants that were responsible for rapid uptake
     of heavy metals and nutrients.  Large amounts of the nutrients subse-
     quently were transferred to cypress roots after decay of the floating
     plants.  Other changes in species composition also were noted.  Tree
     growth and seedling growth were slightly faster in treated domes as
     compared with control domes.  Litterfall was greater in the treated dome,
     but no differences in the decomposition rates of cypress needles were
     noted.  (EPA)
237. Ewel, K.  1976c.  Seasonal Changes in Distribution of Water Fern and
        Duckweed in Cypress Domes Receiving Sewage, pp. 164-170.  In H. T.
        Odum, K. C. Ewel, J. W.  Ordway, and M. K. Johnston  (eds.), Cypress
        Wetlands for Water Management, Recycling and Conservation, Third Annual
        Report.  Univ. of Florida, Gainesville.

        During the spring, summer, and fall, the non-rooted, floating plant
     cover in two cypress domes which had been receiving secondarily treated
     sewage consisted almost entirely of two duckweed species, Lemna perpusilla
     and Spirgdela a 1 i go rh j. za.  Small patches of Azqlla carp 1 iniana, a  small
     floating water fern, was also found, usually associated with a patch of
     emergent vegetation.  There were no floating plants on  the surface of the
     central area of a cypress dome which has had groundwater pumped into it
     weekly, although both Lemna and Azolla are common around the edge. Only
     Lemna and Spirodela are found on the undisturbed dome.  During the winter,
     Azolla. increased its percentage in coverage in both domes significantly.
     This change may be due primarily to a change in light intensity.   Consid-
     ering both the nitrogen-fixing ability of Azolla and the dependence of
     Lemana on a nitrate source, the shift in species composition may clearly
     have important consequences on the nutrient cycling role played by the
     plants.
238.  Ewel, K.C.  1979.  Effects of Sewage Recycling on Structure  and Function
        of Cypress Ecosystems.  In; J.C. Sutherland and R.H. Kadlec  (eds.),
        Freshwater Wetlands and Sanitary Wastewater Disposal.  Abstracts  of  a
        conference held 10-12 July 1979 at Higgins Lake, MI.

        Cypress-dominated ecosystems are common  in Florida  and the Southeast in
     sites where standing water during part of the year reduces  competition
     from other tree species, and occasional  drawdowns permit germination.
        Cypress domes, or ponds, have been found to be able to absorb nutrients
     from secondarily treated sewage applied  at  the rate of 2.5  cm/wk.  This
     loading rate converts the oligotrophic cypress pond into a  duckweed-cover-
     ed, virtually anaerobic body of water.   However, gross and  net  primary
     productivity rates are increased and decomposition rates are  not affected.
     Under-story vegetation composition is not affected.  Most of  the phosphor-
     us discharged into the cypress pond is apparently retained  in the  organic
     sediments and underlying clay layers.
        Cypress strands, which are slowly flowing bodies of water  dominated  by
     cypress trees, area also being investigated for their potential in treat-

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     ing sewage because of the greater land area they cover.  Examination  of
     sites where sewage enrichment has occurred for forty years indicates  that
     increased tree growth rates can be sustained.  Investigations are underway
     to determine area needed for uptake of nutrients, heavy metals, and bac-
     teria.  (AA)
239. Ewel, K. C. , and S. E. Bayley.  1976.  Waldo, A Strand Receiving Sewage,
        pp. 759-767.  In H. T. Odum, K. C. Ewel, J. W. Ordway, and M. K.
        Johnston (eds.), Cypress Wetlands for Water Management, Recycling, and
        Conservation.  Third Annual Report.  Center for Wetlands.  Univ. of
        Florida.  Gainesville.
240. Ewel, K. C., and H. T. Odum.  1978.  Cypress Swamps for Nutrient Removal
        and Wastewater Recycling, pp. 181-198.  In M. P. Wanielista and W. W.
        Eckenfelder, Jr. (eds.), Advances in Water and Wastewater Treatment:
        Biological Nutrient Removal.  Ann Arbor Science Publishers, Inc., Ann
        Arbor, HI.

        This paper is an overview of research conducted on the effect of
     addition of sewage to cypress dome wetlands in Florida.  This appears to
     be an ecologically and economically suitable method for tertiary treat-
     ment.  Growth of cypress trees was increased greatly, but subcanopy
     vegetation was not altered to a similar degree.  No increases in nitrogen
     or phosphorus in groundwater were observed.  Also, no higher rate of
     export of encephalitis would be expected from a treated wetland, as
     opposed to a natural cypress swamp.  The treatment method is considered
     to be economical because the "ratio of solar energy to dollar costs" is
     high.
241. Ewel, K. C., and H. T. Odum.  1979.  Cypress Domes: Nature's Tertiary
        Treatment Filter, pp. 103-114.  In W. E. Sopper and S. N. Kerr  (eds.).
        Utilization of Municipal Sewage Effluent and Sludge on Forest and
        Disturbed Land.  The Pennsylvania State University Press, University
        Park.

        This paper is a summary of 4 years of research concerning the effects
     of additions of treated wastewater to cypress dome wetlands in northern
     Florida.  It was concluded that these systems are capable of effective
     tertiary wastewater treatment.  Domes are common in Florida, and the
     vegetation already is adapted to high water levels.  The ability to deal
     with elevated nutrient loads is related to:

        0 A continuous cover of duckweed causing rapid nutrient removal;

        0 Uptake of nutrients deposited in sediments by duckweed and by cy-
          press roots and incorporation in wood and leaves; trees may absorb
          up to 10% of the nutrient load;

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        0 Phosphorus seems to adsorb on clays beneath the dome; the  domes  also
          appear to have an additional geological mechanism of precipitation
          of phosphate pebbles*

     Overall, the ability of a dome to treat sewage is best evaluated by
     considering  the "geologic framework within which the ecosystem
     operates/" and "taking advantage of long-term adsorptive and concen-
     trating properties."  It is suggested that the use of domes for treatment
     of wastewater may be a viable alternative for small communities whose tax
     base is insufficient to support high-technology treatment systems.
242. Farnham, R.S.  1974.  Use of Organic Soils for Wastewater Infiltration,  pp.
        111-118.  In:  A.R. Aandahl (ed.), Histosols: Their Characteristics,  Use
        and Classification.  Soil Sci. Soc. Am. Spec. Publ. No. 6.
243. Farnham, R.S., and D.H. Boelter.   1976.  Minnesota's Peat Resources:   Their
        Characteristics and Use in Sewage Treatment, Agriculture,  and Energy,
        pp. 241-255.  In; D.L. Tilton,  R.H. Kadlec, C.J. Richardson  (eds.),
        Proceedings of a National Symposium on Freshwater Wetlands and  Sewage
        Effluent Disposal.  University  of Michigan, Ann Arbor.

        Minnesota has 3 million ha (7.5 million acres) of peatlands, the  most  of
     any state in the conterminous United States.  The most extensive areas of
     these peatlands are located in the large glacial  lake plains  in northern
     Minnesota.  A few of the largest areas are vast expanses of peat ranging  up
     to 200,000 ha in size and only a very small percent of them are presently
     being utilized for agriculture or  forestry.
        One of the more important resources of peatlands is water  and the head-
     water areas for several major river basins are located in the northern for-
     ested regions of Minnesota on these peat-covered wetlands.  The hydrologic
     role of peatlands may not be as significant as once thought.  Rather than
     being giant sponges soaking up snowmelt and early spring rains  and gradually
     releasing runoff through the summer, peatlands often play a less significant
     role in the seasonal distribution  of runoff with  a large portion of  the
     annual flow occurring prior to June 15.
        Drained and fertilized, these organic soils are productive for  both field
     and forest crops.  They are particularly suited to the production  of vegeta-
     tive crops, seed crops, forage crops, wild rice and other specialty  crops
     although their present use for these crops is limited.
        The unique physical properties  of peat materials, especially Sphagnum
     types, suggest several potential uses for harvested peat.  Their high water
     storage capacity and low density makes them very  useful as a  soil  amendment
     to improve the physical properties of mineral soils or as a horticultural
     medium for plant growth.  The relative high caloric values of peat have led
     to their use as fuel in Europe and similar potentials exist in Minnesota.
        Peat materials have been used successfully as  filter systems for  treat-
     ment of campground sewage wastes in several national forests.  Renovation of
     wastewaters is quite good using a  combinatin of the filtering and  absorbing
     action of the peat and the harvest of plants growing on the filter beds for
     assimilation and removal of nutrients.  (AA)

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244. Farnham, R.S., and J.L. Brown.   1972.  Advanced Wastewater Treatment Using
        Organic and Inorganic Materials/ Part I.  Use of Peat  and Peat-sand
        Filtration Media, pp. 271-286.  In; Fourth Int. Peat Congr. Proc.,
        Ontaniemi, Finland.
245. Ferrara, R.A., and D.R.F. Harleman.   1978.  A Dynamic Nutrient Cycle Model
        for Waste Stabilization Ponds.  Kept. No. 237 R78-43.  Massachusetts  In-
        stitute of Tech., Cambridge.  351 pp.

        Stabilization ponds provide a simple, economic, and effective method  of
     waste treatment.  Due to their dynamic nature, there is a critical  need  for
     a reliable, predictive method for the design and analysis of stabilization
     ponds that goes beyond the classical BOD approach and considers biological
     and nutrient dynamics.  Existing hydraulic models are evaluated and improved
     dispersion model characterized by an active flow zone and a return  zone  is
     developed and verified.  It is shown it is appropriate to assume fully mixed
     conditions for most existing unbaffled ponds.  A biogeochemical model con-
     taining nine state variables describing the transformations of three ele-
     ments - carbon, nitrogen, and phosphorus - is developed.  The model reflects
     the cycling of elements between organic and inorganic forms and is  capable
     of predicting oxygen demand, fecal coliform, nitrogen, and phosphorus con-
     centrations on a time-variable basis. Application of the model at existing
     treatment sites has indicated the capability of the model structure to ade-
     quately simulate the stabilization pond environment. (NT)
246. Fetter, C.W., and R.G. Holzmacher.  1974. Groundwater Recharge with Treated
        Sewage.  J. Water Pollut. Control Fed.  46(2):260-270.
247. Fetter, C.W., Jr., W.E. Sloey, and F.L. Spangler. 1976a.  Potential Replace-
        ment of Septic Tank Drain Fields by Artificial Marsh Wastewater Treatment
        Systems.  Groundwater 14(6)-.396-402.

        Unchlorinated primary effluent was applied to an artificial marsh in an
     experimental facility ay Seymour, Wisconsin (population 2,257), during the
     period 21 August to 4 November 1975.  The marsh consisted of shallow trench-
     es lined with PVC plastic and filled with gravel and handplanted specimens
     of bulrush (Scirpus validus).  The vegetation and microbiota had been
     established previously during two seasons of experiments with secondary
     effluent.  Effluent was applied at an average rate of 2.5 m /day,
     which yielded a loading factor of 29 1/m^ and a retention time of
     about 10 days.  Reductions in BODs (77%), COD (71%), orthophosphate
     (35%),  total phosphorus (37%), nitrate (22%),  and coliform bacteria
     (99.9%) were reported.  The researchers recommended the use of small-scale
     waste treatment systems at summer cottages, camping areas, resorts, and
     roadside rest areas where effluent would be applied only during the grow-
     ing season.  A proposed design for such a treatment system is included.
     (EPA)

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248. Fetter, C. W., Jr., W. E. Sloey, and F. L. Spangler.  1976b.  Wastewater
        Treatment by Natural and Artificial Marshes.  U.S. National Technical
        Information Service.  Pub. No. PB-259-992.  Springfield, VA.  180 pp.

        The feasibility of using marshland as a means of purifying sewage-works
     effluent was investigated, using artificial marshes in plastic-lined
     excavations, and a natural marsh in Wisconsin already receiving water
     polluted from a sewage-works outfall.  The level of improvement in water
     quality suggests that the process may be acceptable for certain applica-
     tions .  Harvesting of vegetation, however, was not a practicable method of
     phosphorus removal since the marsh was found to absorb phosphorus during
     the growing season and to release it at other times.  It is concluded that
     marsh treatment may be a practical means of dealing with temporary over-
     loads of existing sewage-treatment systems during the summer.  (AL)
249. Fetter, C. W., Jr., W. E. Sloey, and F. L. Spangler.  1977.  Biogeo-
        chemical Studies of a Polluted Wisconsin Marsh, pp. 13-49.  Pro-
        ceedings of the First Annual Wisconsin Water Resources Conference,
        held 11 February 1977 at Stevens Point, WI.
250. Fetter, C. W., Jr., W. E. Sloey, and F. L. Spangler. 1978a.  Biogeo-
        chemical Studies of a Polluted Wisconsin Marsh.  J. Water Pollut.
        Control Fed.

        This study concerns the effect of a Typha dominated marsh in Wisconsin
     on BOD, coliform bacteria, COD, nitrate, turbidity, suspended solids,
     total phosphorus, and orthophosphate.  (WE)
251. Fetter, C. W., Jr., W. E. Sloey, and F. L. Spangler.  1978b. Use of a
        Natural Marsh for Wastewater Polishing.  J. Water Pollut. Control Fed.
        50(2): 290-307.

        The water quality of a stream flowing through a Tygha marsh was studied
     for a 15-month period.  During low flow periods, as much as 50% of the
     wastewater monthly streamflow was discharge from a wastewater treatment
     plant.  During passage through the marsh there were significant reductions
     in concentrations of biochemical oxygen demand  (BOD) (80.1%), coliform
     bacteria (86.2%), nitrate (51.3%), chemical oxygen demand  (43.7%), turbid-
     ity (43.5%), suspended solids (SS) (29.1%), total P (13.4%), and ortho-
     phosphate (6.4%).  An estimated mass balance of P indicated the marsh
     might retain one-third of the entering P over an annual cycle.  One source
     of P removal appears to be preciptation into the sediments.  Because pass-
     age through a marsh will reduce the BOD, P, N and SS, discharged effluents
     may not necessarily need to meet strict water quality standards before
     entering the system.  (AA)
252. Findlay, C. R.  1973.  Salmonella in Sewage Sludge.  Part II.  Multipli-
        cation.  Vet. Rec. 93:102-103.

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253. Finn/ J.T. and T.M. Leschine, 1980.  Does
        Shellfish Production.  An Application
        Management  4(3):193-203.
 Salt Marsh Fertilization Enhance
of Flow Analysis.  Environmental
        Flow analysis was used to determine that adding nitrogen  fertilizer  to
     Spartina salt marsh sediment was not an efficient way to enhace  shellfish
     growth.  Nitrogen flow models for Barataria Bay salt marsh,  Louisiana,  and
     Sippewissett Marsh, Cape Cod, indicated that in both locations only  3%  of
     added N reached the shellfish, despite the great physical differences and
     despite the inclusion of considerably more milcrobial processing  of N in
     the Barataria Bay model.  The most efficient K transfer would have to by-
     pass Spartina growth, decomposition, and detijital feeding.
254. Fitzgerald, M. G.  1980.  Anthropogenic Influence on the Sedimentary
        Regime of an Urban Estuary-Boston Harbor.  Ph.D. Diss.  Dept. of
        Geology and Geophysics, Woods Hole Oceanographic Institution, Woods
        Hole, MA.  300 pp.  (Available as Woods Hole Oceanographic Inst. Rept.
        No. 80-38.)

        Boston Harbor (and its approaches) is a glacially carved, tidally
     dominated estuary in western Massachusetts Bay.  Characterized by  low
     river discharge and significant human impact, the harbor is typical of
     many bays and estuaries along the New England coast.  Modern organic-rich
     sediments are accumlating in several depocenters at an estimated rate of
     0.2-0.3 cm/yr.  The high organic matter content of these modern sediments
     is attributed in part to anthropogenic wastes.  Wastes rich in trace
     metals are discharged into the harbor from the sewage treatment facility
     at Deer Island and from numerous point sources in the Inner Harbor.
     Trace metal profiles in the bottom sediments reflect the increasing use
     and discharge of these metals during the past 100 years.  Large vari-
     ations in several cores may be related to circulation changes.  In the
     water column, silt and clay sized mineral grains are suspended together
     in organically bound agglomerates.  The organic film binding these par-
     ticles is a by-product of biological activity and aids in the deposition
     of particulates and pollutants.  (NT)
255. Fitzgerald, P.R. 1981.  Helminth and Heavy Metals Transmission  for Anaero-
        bically Digested Sewage Sludge.  Pub. No. EPA-600/S2-81-924.  U.S. En-
        vironmental Protection Agency.  Cincinnati, OH.

        This summary discusses the findings of a study designed to determine
     the transmission to an animal host of the ova of the nematode worm Ascaris
     sp. that have survived through a modern sewage treatment process and are
     present in the sludge.  Four large experiments and three smaller ones
     involving 178 specific pathogen-free (SPF) pigs were used.  Natural trans-
     mission of Ascaris sp. from soil treated with liquid anaerobically
     digested sewage sludge that had been stored for several years occurred in
     a few pigs in each of four experiments.  Also, natural transmission from
     Nu-Earth, a dried, stored sewage sludge, also occurred in pigs  that were
     exposed to this material by contact in the pens.  In general, ova in
     anaerobically digested sludge or in Nu-Earth remained unembryonated until

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     after they were exposed to the air.  Within 6 weeks after exposure to air,
     the ova began to embryonate, and thereafter, a small percentage of the ova
     that embryonated became infective for pigs.
        The occurrence of heavy metals in the tissues of swine held in pens
     treated with anaerobically digested sludge or Nu-Earth, which originated
     from a large municipality, was also studied.  Chemical analyses of kid-
     neys, livers, hearts, diaphragm muscles, and bones were conducted to
     determine the quantities of the heavy metals cadmium, zinc, copper, iron
     lead, chromium, and nickel that were present in the tissues following
     exposure of the pigs to different amounts of the sewage products in or on
     the soil.  Only cadmium accumulated to a significant degree in some
     tissues of swine exposed to sludge containing heavy metals.  No physiolog-
     ical or pathological changes associated with exposure to the sludge
     material were detected.  Examination of visceral fat from control and
     experimental pigs indicated that there was no unusual accumulation of
     organic compounds including polychlorinated biphenyls (PCB's) and the
     insecticides Heptachlor and Dieldrin.
256. Flemer, D.A.  and D.R. Heinle.  1974.  Effects of Wastewater on Estuarine
        Ecosystems.  Publication 33.  Chesapeake Research Consortium, Inc.,
        Baltimore, MD.  33 pp.  (Also published as Maryland Univ. (Solomons)
        Natural Resources Inst. Ref. No. 74-79.

        Recent increases in algae, measured as concentration of chlorophyll a,
     in the Patuxent River estuary appear to have occurred as a consequence of
     increased loading by sewage in the upper watershed.  The increases in
     concentration of chlorophyll were observed downstream from the turbid
     sediment-trap portion of the estuary, an impact distant from the points
     of addition.   Primary production, measured by the 14C method, increased
     also as did zooplankton biomass.  Samples of water from the Sandy Point
     area of the upper Chesapeake Bay were enclosed in 750-liter (200 gallon)
     polyethylene  microcosms, enriched with treated sewage, and ensuing events
     followed.  While the predictions from the microcosms have been fully
     validated, clear effects on several parameters were noted.  Phosphate
     appeared to be the major limiting nutrient in these experiments.  En-
     riched microcosms developed higher concentrations of zooplankton and
     chlorophyll a and rates of primary production, permitting predictions of
     effects of the sewage treatment plant under various loads.  (NT)
257. Folsom, B. L., Jr., C. R. Lee, and D. J. Bates.  1980.  Influence of
        Disposal Environment on Availability and Plant Uptake of Heavy Metals
        in Dredged Material.  Tech. Rept. U. S. Army Corps of Engineers
        Waterways Experiment Station, Vicksburg, MS.
258. Fox, J. C., and P. R. Fitzgerald.  1979.  The Presence of Giardia lamblia
        Cysts in Sewage and Sewage Sludges from the Chicago Area.  In W.
        Jakubowski and J. C. Huff (eds.), Waterborne Transmission of
        Giardiasis.  EPA-600/9-79-001, U.S. Environmental Protection Agency,
        Cincinnati, OH.

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259. Fox, R. L., and E. J. Kamprath.   1971.  Adsorption  and Leaching of  P  in
        Acid Organic Soils and High Organic Matter Sand.  Soil  Sci.  Soc. Amer.
        Proc. 35:154-156.
260. Frederickson, L. 1978.  Lowland Hardwood Wetlands:  Current  Status  and
        Habitat Values for Wildlife,  pp. 296-306, In; P.E. Greeson, J.R.
        Clark, and J.K. Clark (eds.), Wetland Function and Values - The  State
        of our Understanding.  Proceedings of the National Symposium on  Wet-
        lands, Buena Vista, Florida.  Amer. Water Resources Assoc. 7-10
        November 1978.
261. Friend, Milton.  In press.  Wildlife Health Implications of Sewage Dis-
        posal in Wetlands.  In  Ecological Considerations in Wetlands Treat-
        ment of Municipal Wastewaters. Proceedings of a workshop, June 23-25,
        1982. University of Massachusetts, Amherst MA.  US Fish and Wildlife
        Service and US Environmental Protection Agency.
262. Fritz, W. R., and S. C. Helle.  1977.  Tertiary Treatment of Wastewater
        Using Cypress Wetlands.  National Science Foundation.  Updated Pre-
        liminary Envir. Rept. 76-23276.  96 pp.

        This report deals with the results of study using cypress domes  for
     tertiary treatment of secondary effluent.  Conclusions include:  1)  cost
     comparisons with physical, chemical, spray irrigation and other  wetland
     treatment methods show cypress domes, to be cost effective if favorable
     site specific conditions exist, 2) feasible loading rates and physical
     size of domes restrict applicability to small wastewater systems, and 3)
     analysis reveals that wetland treatment required substantially less
     purchased energy and chemicals.  No significant detrimental effects to
     dome ecology have been discovered.  (WE)
263.  Fritz, W.  R.,  and S. C. Helle.  1978a. Cypress Wetlands as a Natural
        Tertiary Treatment Method for Secondary Effluents.  Boyle Engineering
        Corp.,  Orlando, FL.  National Science Foundation Summary Rept.   14 pp.

        The concept of using cypress wetlands as a natural tertiary treatment
     mechanism for  domestic wastewaters is examined.  Previous studies have
     shown that cypress domes supplied with sewage effluent effectively
     treated the effluent well within acceptable tertiary treatment standards
     with no significant adverse effect on the environment.  As a result of
     these findings,  a three-phase approach was developed for an applied
     engineering science suitable for implementation.  This article addresses
     Phases 1 and 2:  development of conceptual techniques for using cypress
     wetlands for tertiary treatment and determination of the feasibility of
     utilizing the  method.  (NT)

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264. Fritz, W. R., and S. C. Helle.  1978b.  Cypress Wetlands  as  a Natural
        Treatment Method for Secondary Effluents, pp. 69-81.   In  M. A.  Drew/
        (ed.),  Environmental Quality through Wetlands Utilization.   Pro-
        ceedings of a symposium held 28 February - 2 March  1978,  at Tallahas-
        see, FL.  Sponsored by the Coordinating Council on  the Restoration  of
        the Kissimmee River Valley and the Taylor Creek-Nubbin Slough Basin.
        Tallahassee, FL.

        Research by the Center for Wetlands, University of  Florida demonstrates
     that cypress wetlands can be a natural tertiary treatment mechanism for
     domestic wastewaters.  Their studies show that wetlands provide  excellent
     nutrient removal with no significant adverse environmental effects. Boyle
     Engineering Corporation has been awarded a National Science  Foundation
     grant to study the research and convert it into an applied engineering
     science.  Boyle's approach has been to: 1) examine the various concepts of
     using cypress wetlands for tertiary treatment, 2) explore the feasibility
     of each concept, and 3) develop procedures and preliminary regulations for
     implementation.
        Thus far, the study has demonstrated that cypress wetlands offer a
     viable tertiary treatment alternative for many potential  users.  The
     research to date has concentrated on using isolated cypress  "domes" in-
     volving ponding and percolation of wastewaters.  The treatment results,
     reliability, energy conservation potential, and environmental outlook  for
     the method are quite favorable.  Federal and state regulatory agencies
     cautiously support wetland treatment systems, allowing demonstration pro-
     jects, but are not yet ready to permit widespread implementation.   Cost
     analyses have shown cypress domes to be cost competitive  if  the  wetlands
     are favorably located.  A survey of treatment plant locations with ade-
     quate cypress wetlands is being conducted to determine the availability of
     application throughout the State of Florida.  (AA)
265. Fritz, W. R., and S. C. Helle. 1978c.  Cypress Wetlands  for  Tertiary
        Treatment.  Boyle Engineering Corp., Orlando,  FL.   National Science
        Foundation Final Report.   114 pp.

        The report contains a summary of  studies  of impacts on vegetation in
     cypress domes that receive wastewater.  In general,  cypress  trees  appear
     to suffer little negative effects, and in fact may  even  grow more  rapidly
     (in one study they grew 2.6 times as  fast).  Other  studies have shown
     that needle tissue levels of N and P  and seed production increased as a
     result of wastewater application.  Possible  negative effects could in-
     clude the requirement of cypress seedlings for periodic  dry  periods for
     germination.  Other impacts on vegetation include increased  abundance of
     duckweed, associated with nutrient and heavy metal  uptake by those
     plants.  Cypress roots may absorb a  large percentage of  nutrients  when
     the duckweed dies off.  Other impacts include creation of anaerobic
     conditions during duckweed population declines and  possible  changes in
     denitrification rates, but these have been determined not to cause
     significant changes in the ecology of the domes.   (EPA)

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266. Fritz, W. R., and S. C. Helle.  1978d.  Tertiary Treatment of Wastewater
        Using Cypress Wetlands.  Boyle Engineering Corp., Orlando, FL.
        National Science Foundation Final Report No. 0-N04-100-50. 114 pp.

        Intense field investigation, testing, and research were conducted for
     the purpose of providing tertiary treatment utilizing cypress domes.
     Presented in four annual reports, the research has  demonstrated high
     quality treatment using cypress domes, while recycling water and helping
     to protect the environment from periodic drought conditions.  Treatment
     concepts and results, cost-effectiveness, energy and environmental
     considerations, reliability, regulatory agency aspects, availability, and
     a proposed rule for application of domestic wastewater to cypress wetlands
     are discussed.  Also included are considerations for an engineering design
     of a cypress wetland tertiary treatment system and  the dissemination of
     information through seminars and conferences, publications, workshop, and
     reports.  (NT)
267. Fritz, W. R., and S. C. Helle.  1979a.  Cypress Wetlands for Tertiary
        Treatment, pp. 75-81.  In Bastian, R. K., and S. C. Reed (Project
        officers).  Aquaculture Systems for Wastewater Treatment: Seminar
        Proceedings and Engineering Assessment.  U.S. Environmental Protection
        Agency, Office of Water Program Operations, Municipal Construction
        Division.  Washington, D.C.
268. Fritz, W. R., and S. C. Helle.  1979b.  Cypress Wetlands:  A Natural
        Tertiary Treatment Alternative.  Water and Sewage Works 126(4):58-60.
269. Fritz, W. R., and S. C. Helle.  1979c.  Natural Tertiary Treatment of
        Secondary Effluents by Wetlands in Florida.  In; J.C. Sutherland and
        R.H. Kadlec (eds.), Wetland Utilization for Management of Community
        Wastewater.  Abstracts of a conference held 10-12 July 1979 at Higgins
        Lake, MI.

        In Florida, two forms of cypress wetlands are found to be suitable for
     tertiary treatment.  Cypress domes are relatively small (1-25 acres) wood-
     ed ponds which collect runoff from surrounding higher grounds and allow it
     to percolate slowly into underlying soils and groundwaters.  A strand is a
     diffuse freshwater stream flowing through a shallow cypress forested
     depression on a gently sloping plain.
        The Center for Wetlands1 research has demonstrated that cypress domes
     offer a viable tertiary treatment alternative for many potential users.  A
     demonstration project using cypress domes attained 98% total nitrogen and
     97% total phosphorus removal.  The concentrations of the nutrients, coli-
     forms, viruses, heavy metals and other monitored parameters in the ground-
     waters remained virtually unchanged from background levels.  However, an
     investigation by Boyle Engineering Corporation also revealed that these
     ponding and percolation wetland systems are limited by their physical size
     and availability.

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        A survey of nearly one-half the wastewater treatment facilities in
     Florida showed that while only 3% of the state's wastewater effluent had
     the potential of being treated with cypress domes, over 28% of the efflu-
     ent had the potential of being treated with cypress strands and other
     flow-through forested wetlands.  Thus, it becomes apparent that flow-
     through systems offer more promise for use and should be investigated
     further.
        Two and one-half years of research and testing (to quantify design
     parameters) will be conducted in conjunction with the Center for Wetlands
     at two wetland sites near Jasper and Waldo, Florida.  In both instances,
     municipal treatment plants discharge secondary effluents into wetlands.
        A number of water quality and vegetation response parameters are being
     monitored and analyzed at several locations within the study and control
     areas.  Among the water quality parameters being studied are nutrients,
     heavy metals, and coliform bacteria.  Vegetation analyses will include
     determination of tree growth rates, litterfall rates and study of succes-
     sional changes occurring in the vegetation.  Very early results indicate
     that phosphorus is not removed from the wastewater as rapidly as nitrogen
     and thus may be the controlling factor.  (AA)
270. Fritz, W. R., and S. C. Helle.  1980a.  Tertiary Treatment of Wastewater
        Using Flow-Through Wetland Systems, Status Report 3.  Boyle Engineer-
        ing Corp., Orlando, FL. 79 pp.

        The monitoring operations and a water budget analysis of a
     flow-through wetland system for wastewater treatment near Jasper, FL,
     named Basin Swamp are evaluated.  The purpose of the monitoring program
     is to identify the effects of wastewater on the swamp as well as the
     effects of the swamp on wastewater effluent.  In addition, points where
     the magnitude of these effects diminish to background levels are being
     identified.  An effort is being made to determine which effects are
     acceptable to the wetland environment and public health.  Monitoring of
     surface and subsurface waters included studying water chemistry, nutri-
     ents, heavy metals, vegetation, and coliform bacteria.  Preliminary
     results show: 1) a reduction of total nitrogen to near background levels?
     2) a reduction of phosphorus levels; 3) that the application of effluent
     to cypress strand may be harmful to trees; 4) that the total and fecal
     coliform levels appear to be readily reduced; 5) that nutrients and
     coliform bacteria penetrate shallow groundwater levels; and 6) that no
     significant amounts of heavy metals are being applied to Basin Swamp.  To
     supplement various monitoring programs, a water budget analysis was
     refined and updated to include data collected from weekly monitoring
     operations.  Results significantly improved the accuracy of the water
     budget analysis.  (NT)
271. Fritz, W. R., and S. C. Helle.  1980b.  Tertiary Treatment of Wastewater
        Using Flow Through Wetland Systems, Status Rept. 2.  Boyle Engineering
        Corp., Orlando, FL.  115 pp.

        Recent research has demonstrated that certain types of wetlands  can
     provide tertiary treatment for effluents from secondary wastewater  treat-

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     ment  facilities.   To identify and quantify design parameters for imple-
     menting  such  systems,  a  study is  being conducted at a north Florida site
     near  Jasper.   This report  describes the development of the monitoring
     system and  its preliminary mapping.  It presents a brief  history of the
     project,  the  study approach,  the  research plan,  preliminary results, and
     recommendations for monitoring future periods.   Reports for each of five
     monitoring  programs are  included  in the appendices:  water chemistry;
     bacterial sampling;  vegetation distribution in West Swamp; and an analy-
     sis of cypress tree growth rate.   The appendices also include research on
     a water budget study,  development of a water budget model, instructions
     for a water monitoring program, and output from  a water budget program.
     Findings  indicate  that nitrogen appears to be removed to  below AWT levels
     with  a loading rate of about  100  acres per mgd.   Phosphorus is not
     removed as  readily.  Applying raw and/or primary effluent to a cypress
     stand appears  to be harmful to the trees whereas applying secondary
     effluent  appears to be beneficial.   (NT)
272. Frook, D. S.   1974.  Clarification  of  Sewage Treatment Plant  Effluent
        with Daphnia pulex.  Master's Thesis.  Univ.  of  Toledo.  OH.
273. Frykberg, W. R., C. J. Goodnight,  and P.  G. Meier.   1975.  Limnological
        Investigation of the Muskegon County,  Michigan, Wastewater  Storage
        Lagoons.  Phase 1.  EPA/600/3-75-009.  U.S. Environmental Protection
        Agency, Interim Rept.

        The limnology of two 850 acre wastewater storage  lagoons was  inves-
     tigated from September 1973, shortly after the initial  filling,  through
     August 1974.  Special emphasis was placed upon the biological  aspects of
     these lagoons.  During the period of study, the East Lagoon received most
     of the wastewater, while the West Lagoon  received mostly land  drainage
     and seepage water.  Due to these different waters in each  lagoon,  dif-
     ferences in most of the parameters were apparent between the two lagoons.
     Chironomid larvae comprised virtually all of a scant benthic population
     in both lagoons, with different dominant  genera in each body of  water.
     The dominant zooplankton in the East Lagoon were cyclopoid copepods, with
     Cyclops yernalis the most common form, while calanoid copepods and
     Daphnia were dominant in the West Lagoon.  Chlorophyll a and primary
     productivity were measured and numerous physical-chemical  parameters were
     also investigated.  (NT)
274. Frykberg, W. R., C. Goodnight, and P. G. Meier.  1977.  Muskegon,
        Michigan Industrial-Municipal Wastewater Storage Lagoons: Biota and
        Environment.  U.S. Environmental Protection Agency, Final Rept.
        EPA/600/3-77-039.  89 pp.

        A limnological investigation was carried out on two 344 hectare
     (850 acre) industrial-municipal wastewater storage lagoons from August
     1973 until August 1975.  Besides monitoring physical and chemical par-
     ameters during the period of the initial filling, the biological com-

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     munity was critically examined for the purpose of documenting ecological
     succession over this two year period.  In general, the lagoons remained
     aerobic, well mixed vertically, and slightly alkaline.  The low trans-
     parency within the lagoons was an important factor which limited the
     phytoplankton population and excluded rooted aquatics and benthic algae.
     Ample nutrients were present for algal demands.  The lagoon's phyto-
     plankton-protozoan assemblage was extremely variable with respect to
     total abundance distribution.  The zooplankton community was composed of
     fourteen species of free living crustaceans and four species of rotifers<
     The benthic fauna consisted of a small number of organisms representing
     only a few taxonomic groups.  (NT)
275. Fuller, R.J., and D.E. Glue.  1980.  Sewage Works as Bird Habitats in
        Britain.  British Trust for Ornithology, United Kingdom.  Biological
        Conservation  17(3):165-182

        This research report demonstrates that sewage treatment works are
     essentially good bird habitats.  Bird communities associated with dif-
     ferent sewage disposal systems in the United Kingdom are described in
     relation to the components of each system.  Ornithological gains and
     losses associated with recent developments in sewage treatment methods
     are discussed.  Modern sewage treatment works, which are smaller in area
     than sewage farms, support smaller and less varied populations of wetland
     species.  However, modern sewage treatment works can be important bird
     habitats, particularly as feeding sites for several passerine species.
     Systems with percolating filters and tertiary treatment by surface irri-
     gation will support the most varied bird communities.  (EPA)
276. Fuller, R.J. and D.E. Glue, 1981.  The Impact on Bird Communities of the
        Modernization of Sewage Treatment Works.  Effluent and Water Treatment
        Journal 21(1):27-31.

        The main ornithological characteristics of the obsolete sewage farms in
     Great Britain are described, and the ornithological potential of modern
     sewage works is discussed.  Sewage treatment systems in Great Britain have
     provided some of the most spectacular examples of birds utilizing human
     artifacts.
277. Gallagher, J. L.  1978.  Decomposition Processes: Summary and Recommen-
        dations,  pp. 145-151.  In R. E. Good, D. F. Whigham, and R. L.
        Simpson (eds.), Freshwater Wetlands: Ecological Processes and Manage-
        ment Potential.  Academic Press, Inc., New York, NY.
278. Gallagher, J. L., and H. U. Kibby.  1980.  Marsh Plants as Vectors  in
        Trace Metal Transport in Oregon Tidal Marshes. Am. J. of Bot. 67(7):

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279. Gambrell, R.P.  1977.  Transportation of Heavy Metals and Plant Nutrients
        in Dredged Internal Sediments as Affected by Oxidation Reduction Poten-
        tial pH; Volume II, Materials and Methods/Results and Discussion.
        Contract Rept.  D-77-4.
280. Gambrell, R. P., V. R. Collard, C. N. Reddy, and W. H. Patrick, Jr.
        1977.  Trace and Toxic Metal Uptake by Marsh Plants as Affected by Eh,
        pH, and Salinity.  U.S. Army Corps of Engineers, Waterways Experiment
        Station, Dredged Material Research Program Technical Rept. D-77-40.

        A laboratory study was conducted to develop and refine methods for
     growing marsh plants under conditions of controlled pH, redox potential
     (oxidation-reduction conditions) and salinity as well as to determine the
     effects of these physiochemical conditions in the rooting medium on trace
     and toxic metal availability to plants.  Reasonably successful methods
     were developed for growing marsh plants in the experimental systems.  The
     successful methods as well as procedures which were not successful are
     discussed as are techniques for overcoming many of the experimental
     difficulties.
        In laboratory studies using soil suspensions, Distichlis spicata (L.)
     Greene, Spartina alternifIqra Loisel, and Spartina cynpsuroides (L. ) Roth
     were grown in soil suspensions in which treatments included oxidized and
     reduced conditions at two pH levels and two salinity levels.  The effect
     of these soil physiochemical conditions on plant content of mercury,
     lead, cadmium, copper, iron, and manganese was studied.  Lead and cadmium
     uptake by Oryza sativa L. was also studied, but over a range of four soil
     pH levels and six redox (oxidation-reduction) potential conditions.
        Greenhouse studies were conducted using Distichlis spicata, Spartina.
     alterniflora, and Sorghum halepense (L.) Pers. in which oxidation-reduc-
     tion conditions were controlled in unstirred soil material.  The plant
     content of the trace metals studied in the greenhouse generally supported
     results of laboratory studies where soil suspensions were used as the
     rooting medium.
        In laboratory studies using labeled mercury, the content of this metal
     in marsh plants tended to be enhanced by decreases in salinity and in-
     creases in pH.
        Although results with lead were inconclusive in laboratory studies,
     lead uptake by lowland rice was enhanced as the soil became more acid.
     In greenhouse studies, an increase in soil oxidation-reduction conditions
     increased plant lead content, but this was attributed to a redox poten-
     tial mediated effect on soil pH.
        Plant cadmium content responded more to a change in the physiochemical
     environment of the rooting medium than did other metals studied.  The
     cadmium content of above ground tissue was consistently and substantially
     increased with an increase in oxidation conditions in all species in both
     laboratory and greenhouse studies.  Maximum cadmium content occurred
     under acid, oxidizing conditions except for one species in which an
     oxidized, weakly alkaline environment favored increased cadmium content.
        The effects of physiochemical conditions on the zinc content of plants
     grown in the greenhouse were studied where the zinc content was found to
     increase with increasing oxidation conditions.
        There was little effect of either pH or oxidation-reduction conditions
     on the copper content of the species studied.

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        Two extractants were used on the soil suspensions following harvest to
     determine the linear relationships between chemical availability and plant
     content of metals in laboratory studies.  Of the metals studied, cadmium
     generally gave the best linear association between measured uptake and
     chemical availability, although there were important species differences.
        Based on the results of this research, it was concluded that soil and
     sediment physiochemical conditions do affect the plant availability of
     trace and toxic metals, and it was recommended that these effects should
     be considered in selecting environmentally sound disposal methods for
     contaminated dredged sediments.  (AA)
281. Garbisch, E. W., Jr.  1977.  Recent and Planned Marsh Establishment Work
        Throughout the Contiguous United States - A Survey and Basic Guide-
        lines, U.S. Army Corps of Engineers, Waterways Experiment Station,
        Technical Kept. D-77-3.  Vicksburg, MS.
282. Garbisch, E. W., Jr.  1978.  Wetland Rehabilitation, pp. 217-219.  In J.
         H. Montanari, and J. A. Kusler (eds.), Proceedings of the National
         Wetland Protection Symposium held 6-8 June 1977 at Reston, Virginia.
         FWS/OBS-78/97.  U.S. Fish and Wildlife Service, Washington, DC.
283. Gaudet, J.J. 1978.  Effect of a Tropical Swamp on Water Quality.  Verhand-
        lungen Internationale Veremiging Fur Theoretische und Unvewandte
        Limnologie,  (20(4):2202-2206

        A papyrus swamp may be effective in removal of organic nutrients  from
     sewage effluents directed into these swamps, but will have little effect
     on the salt content of the effluent if the discharge is at a high rate.
     Water samples collected from a floating papyrus swamp show a decrease
     in major ions on the swamp bottom during the period August-December. Papy-
     rus takes up and accumulates considerable amounts of the major ions.

284. Gearheart, R. A., and B. A. Finney.  1981.  Use of Vascular Plants for
        Treatment and Reclamation of Oxidation Pond Effluent and Non-Point
        Source Pollution Loads.  Presented at the Water Reuse Symposium II.
        Washington, DC.  August 23-28, 1981.

        The City of Arcata is presently funding an alternative wastewater
     pilot project by the State Water Resources Control Board, which will 1)
     evaluate the feasibility of using secondarily treated wastewater to
     enhance the productivity of a freshwater marsh, and 2) to test the
     effectiveness of marshes to reliably treat stabilization pond effluent to
     tertiary standards.  Twelve 0.11 acre (22 feet by 200 feet) experimental
     marshes have been operated with varying flow rates, types and density of
     vegetation, and depth of flow.  The experimental design requires testing
     and evaluation of the optimum aquatic composition, water level control,
     hydraulic toading rate, organic and nutrient loading rates, and manage-
     ment practices for use as a wastewater reclamation process.  Nutrients
     such as ammonia nitrogen, Kjeldahl nitrogen, nitrates, nitrites, and

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     phosphate forms as well as other water quality parameters such as BOD,
     COD, total and fecal coiiform, and fecal streptococcus.  A balance of the
     bio-mass production and its associated nitrogen content will be made.  A
     thorough sampling program of the aquatic plants, including roots and
     rhizomes, phytoplankton, zooplankton, heavy metals, pH, temperature, and
     specific conductivity have been monitored on a regular basis.
        The City of Arcata has also constructed three marshes, adjacent to the
     existing wastewater treatment facility, totaling 35 acres, and a 17-acre
     recreational lake all connected in series with hydraulic alternatives to
     isolate any one of the three marshes from the total system.  The water
     source for this study area will be obtained from James Creek and from a
     well on site for use during dry periods.  One of the water quality
     objectives of this project is to reduce the non-point  source load to
     Humboldt Bay by processing the runoff through wetland  systems.  This
     project, referred to as the City of Arcata's Coastal Conservancy Marsh
     Enhancement Project, involves the restoration and enhancement of 35 acres
     of freshwater marsh and conversion of a sterile catch  basin and a
     closed-out sanitary landfill into a recreational lake would restore
     approximately seventeen acres to a productive aquatic  habitat and the
     landfill conversion, once implemented, would add ten acres to the entire
     project.
        The marsh system and recreational lake are designed as a series of
     units so that continuous and uninterrupted productivity of the aquatic
     systems will be possible at all times.  The input flow to the Marsh
     Enhancement Project will be from James Creek watershed (combined agri-
     cultural and urban non-point sources of pollution) during the wet months
     of the year and from a well with relatively high water quality during the
     dry months of the year where low flow augmentation precludes taking water
     from James Creek. (AA)
285. Gee & Jenson Engineers, 1980.    Water Hyacinth Wastewater Treatment
        Design Manual.  NASA/National Space Technology Laboratories.  NSTL
        Station, Mississippi.

        This handbook on wastewater treatment with water hyacinths has been
     prepared with two objectives in mind.  The primary objective is tp present
     practical procedures necessary to plan, design and construct a wastewater
     treatment system utilizing water hyacinths.  Laboratory and field-scale
     investigations have repeatedly demonstrated that wastewater treatment with
     water hyacinths can be a viable, cost-effective alternative to convention-
     al treatment schemes.  The state-of-the-art has reached a level where
     existing data can be used to develop reliable design requirements and pro-
     cedures, as presented in this manual.
        A secondary objective is to provide a reference on water hyacinths that
     can be used by engineers, designers, researchers, city officials, utility
     personnel, public regulatory agencies and others involved in wastewater
     treatment systems.  A concise summary of past research is presented so
     interested individuals can locate information pertaining to their needs.
     (AA).
286. George, D.B. , 1981.  Lagoons and Oxidation Ponds. J. Water Pollut. Cont.
        Fed.  (Literature Review Issue), 53(6):709-711.

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        A literature review is presented of publications on lagoons and oxida-
     tion ponds published in 1980.   Articles fall into the general categories
     of process performance and biology, upgrading of lagoons/ and process
     models.   Connecticut published standards on lagoon systems for the dis-
     posal of septage.  Several studies dealt with the Dan Region Project, which
     is the largest and most advanced water reuse scheme in Israel which util-
     izes oxidation ponds.  A study on stabilization ponds in Peru examined the
     use of treated wastewater for agricultural purposes.  More stringent
     wastewater treatment plant discharge standards resulted in several studies
     on upgrading existing lagoons.  An in situ method is alum addition,
     another study investigated water hyacinth, and another evaluated the cul-
     turing of fathead  minnows.  Process models included a biogeochemical model
     developed to describe the dynamic responses of carbon, nitrogen, phosphor-
     us, and pathogenic contaminants in facultative wastewater stabilization
     ponds.
287. Giblin, A. E., A. Bourg, I. Valiela, and J. M. Teal.  1980.  Uptake and
        Losses of Heavy Metals in Sewage Sludge by a New England Salt Marsh.
        Am.  J. Bot. 67(7):1059-1068.

        As part of an investigation on the potential of salt marshes to act as
     natural waste treatment systems, the cycling of heavy metals in Great
     Sippewissett Marsh, MA, was studied.  For the last 7 yrs. , varying doses
     of fertilizer containing sewage sludge were added to experimental plots.
     Changes in metal levels in the sediment, grasses, and animals were
     monitored.  Marsh sediments retained 20-35% Cd, 20-50% Cr, 60-100% Cu,
     55-100% Pb, 80-100% Fe, 55-60% Mn and 20-45% of the Zn added in the fer-
     tilizer.  When compared with low marsh, high marsh areas retained a
     significantly greater fraction of all the added metals except Mn.  In the
     treated plots the dominant grasses, Spartina. alterniflora and IS. patens,
     contained elevated levels of Cd, Cr, Cu and Zn.  Recent Spartina litter
     contained elevated levels of Fe, Cu, Cr, and Pb and reduced levels of Cd.
     The mussell Modiqlus demissus exhibited significantly elevated levels of
     Cd, Cr and Cu and reduced levels of Pb compared to control animals.  The
     fiddler crab Uca pugnax showed significantly higher levels of Cd, Cu, and
     Mn.  (BA)
288. Giesecke, F. E., and P. Zeller.  1939.  Treatment of Settled Sewage in
        Lakes.  Bulletin 47, Agricultural and Mechanical College, Texas ASM
        Univ. College Station.
289. Gloyna, E. F., J. F. Malina, Jr., and E. M. Davis.  1976.  Ponds as a
        Wastewater Treatment Alternative.  Water Resources Symp. #9.  Center
        for Water Research, College of Engineering, Univ. of Texas, Austin.
        447 pp.

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 290.  Goldman,  J.  C.   1979.   Outdoor Algal Mass Cultures.  I.  Applications.
         Water  Research 13(1):1-19.

         Giving a  bibliography  of  111 references,  the author  reviews work on
      the mass  culture  of  algae, with particular reference to the  relative
      merits  of existing and new applications,  including production of
      single-cell  protein, aquaculture,  sewage  treatment,  and water renovation.
      (AL)
 291.  Goldman, J.  C.  et  al.   1974.   Inorganic  Nitrogen Removal  in a Combined
        Tertiary  Treatment-marine Aquaculture System I.   Removal Efficiencies.
        Water Res. 8:45-54.
292. Goldman, J. C.,  and J.  H.  Ryther.   1975.  Nutrient Transformations  in
        Mass Cultures of Marine Algae.   J. Env. Eng.  Div.,  ASCE  101:351-364.

        Results from  the continuous  operation  of  2  algal  growth  ponds  con-
     taining mixtures of secondary sewage effluent  and sea  water,  over a
     period of 24 weeks, demonstrated the feasibility of  this  method for
     nutrient removal from wastewater and/or food production for aquaculture
     of shellfish.  Nitrogen removal was highly dependent on operating con-
     ditions and graphs show the effects of dilution  rate and  nitrogen loading
     on nitrogen removal and algal growth.  Removal of nitrogen, through
     evolution of ammonia at the high pH values attained  in the  ponds, was
     enhanced by maintaining ammonium nitrogen as the dominant form in the
     sewage effluent.  Algal yields  of 6 g of particulate carbon/m2/day  we
     obtained.  A bibliography  of 22 references is  appended.   (AL)
293. Goldman, J. C., and J. H. Ryther.  1976a. Development of a Waste Re-
         cycling Marine Aquaculture System.  In J. Tourbier and R. W. Pierson
         (eds.), Biological Control of Water Pollution.  The University Press,
         Philadelphia, PA.
294. Goldman, J. C., and J. H. Ryther.  1976b. Waste Reclamation in an
        Integrated Food Chain System, pp. 197-214.  In J. Tourbier and R. W.   re
        Pierson (eds.), Biological Control of Water Pollution.  Univ. of
        Pennsylvania.  Philadelphia.
295. Goldman, J. C., K. R. Tenore, J.H. Ryther, and N. Corwin.  1974.  Inor-
        ganic Nitrogen Removal in a Combined Tertiary Treatment Marine Aqua-
        culture System.  I. Removal Efficiencies.  Water Resources 8:45-54.

        The increasing awareness that nitrogen is often a key nutrient control-
     ling algal growth in coastal maring waters has led to a concerted effort
     to find ways to remove ammonia and nitrate from wastewaters.  A novel

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     approach to this problem involves the combining of algal and seaweed
     nutrient stripping processes with a marine aquaculture.  Not only is
     nitrogen removed from wastewater, but important commercial shellfish and
     seaweeds are produced.
        A prototype process consisting of growth systems for marine algae, oys-
     ters, and seaweed, joined in series was fed secondarily treated
     wastewater, diluted 1:4 with seawater for 11 weeks during the Summer of
     1972.  During this time 95 percent of the influent inorganic nitrogen was
     removed by algal assimilation.  The oysters in turn removed 85 percent of
     the algae, but regenerated as soluble ammonia 16-18 percent of the nitro-
     gen originally bound in the algal cells.  All of the regenerated nitrogen
     was removed in the seaweed system so that the total inorganic nitrogen
     removal efficiency of the system was 95 percent.  Phosphorus removal on
     the other hand, was not nearly as complete as only 45-60 percent was
     removed.
        The process has the capability of being expanded to include additional
     trophic levels in an integrated and highly controlled food chain system to
     serve the dual function of tertiary wastewater treatment and waste recycl-
     ing through the production of shellfish and seaweeds.  (AA)
296. Goldman, J. C., K. R. Tenore, and H. I. Stanley.  1974.  Inorganic
        Nitrogen Removal in a Combined Tertiary Treatment - Marine Aquaculture
        System.  II.  Algal Bio-assays.  Water Res.  8:55-59.

        Algal bio-assays of samples from the mariculture sewage treatment sys-
     tem showed that seawater dilutions of sewage effluent from which nitrogen
     had been removed, supported no more algal growth than sea water alone.
     Samples from the oyster culture tank contained regenerated nitrogen and
     showed increased algal growth potential, but assays of the effluent from
     the seaweed growth stage confirmed that this regenerated nitrogen had been
     removed.
297. Golueke, C.G.  1979.  Aquaculture in Resource Recovery.  Compost Science/
        Land Utilization, 20(3):16-23.
298. Golueke, C. G., and L. F. Diaz.  1981.  Operating a Solar Aquaculture
        Sewage Treatment Plant.  Biocycle 22(1):38-39.

        Hercules, CA, is a rapidly growing community with a  rapidly  growing
     sewage treatment problem.  In an experiment that could  have  important
     implications for the future of sewage treatment in Hercules  and other
     growing communities, about 25-50% of the city's wastewater is currently
     diverted to a solar aquaculture facility, where water hyacinths and other
     biological agents stabilize the dissolved and  suspended solids  in  waste-
     water.  Principle elements of the facility are a primary tank,  an
     aeration basin, and a water hyacinth lagoon.  Problems  that  have
     developed during five months of operation are  discussed.   (EL)

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 299. Golueke, C. G., and W. J. Oswald.   1965.  Harvesting  and Processing Sew-
         age-grown Planktonic Algae. J. of Water Pollu. Control  Fed.  37:471-99.

         The great unit savings which would result  from the production  and
      separation of algae in large plants should encourage  neighboring  communi-
      ties to merge their sewage flows and pump the material  to  areas of  inex-
      pensive level land.  Here, large, skillfully  managed  systems  could  be  used
      for converting waste nutrients to algae at low unit costs.
         The entire question resolves itself to a final one of whether  or not
      there is a durable and lucrative market for sewage-grown algae.  Should
      such a market exist, communities which are forced by  rigid water  pollu-
      tion control requirements to undertake tertiary treatment  could ill-
      afford to overlook algae production as an early and ultimate  step in their
      waste disposal systems. (AA).
 300. Good, R. E., D. F. Whigham, and R. L. Simpson  (eds.).   1978.  Freshwater
         Wetlands: Ecological Processes and Management Potential.  Academic
         Press, New York, NY.  378 pp.

         Proceedings of the symposium, "Freshwater Marshes: Present Status,
      Future Needs," held in February 1977 at Rutgers University, New
      Brunswick, NJ.  Contains six articles on primary production processes,
      five on decomposition processes, six on nutrient dynamics, and six on
      management potential.
 301. Gordon, M.S., and D.J. Chapman.  1979.  Aquaculture Approaches to Waste-
         water Nutrient Recycling.  Technical Completion Report.  Office of
         Water Research and Technology, Washington, DC.  79 pp.

         Five years of laboratory scale studies were conducted to assess the
      potential of wastewater aquaculture as a means of 1) developing a basis
      for a freshwater aquaculture industry in the U.S., 2) producing salable
      foods or other commodities to improve waste treatment plant economics,
      3) reducing agricultural energy costs by eliminating fertilization needs,
      and 4) improving the quality of waste treatment plant discharges.  Sequen-
      tial monocultures of wastewater based food chains were investigated using
      algae, crustaceans, and fishes.  All wastewater used for the  studies
      was fully secondarily treated, chlorinated, and dechlorinated.  Results
      show that the use of artificial food chains for tertiary wastewater
      treatment is feasible for the reduction of nutrient loading and for the
      production of valuable protein sources.  (NT)


1072.  Gosselink, J.G.  and L. Gosselink.  In press.


 302.  Gosselink, J. G., E. P. Odum, and R. M. Pope.  1974.   The Values of the
         Tidal Marsh.   Sea Grant Publ.  LSU-SG-74-03.  Center for Wetland Re-
         sources, Louisiana State Univ.,  Baton Rouge.  30 pp.

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303. Goulden, C. E.  1976.  Biological Species Interaction and Their Signi-
        ficance in Waste Stabilization Lagoons.  In Ponds as a Wastewater
        Treatment Alternative.  Water Resources Symp. #9.  Center for Water
        Research, College of Engineering, Univ. of Texas.  Austin.
304. Goulder, R., A. S. Blanchard, P. L. Sanderson, and B. Wright.  1980.
        Relationships Between Heterotrophic Bacteria and Pollution in an
        Industrialized Estuary.  Water Research 14(6):591-602.

        The effects of industrial effluents and bacterial populations in the
     estuary near Humber, England, were studied.  In general, growth of
     bacteria in the estuary corresponded to the amount of organic pollution
     in the water; no widespread inhibition of growth due to industrial wastes
     was found.  At effluent discharge sites of a metal refinery, however,
     heavy metal concentrations affected bacterial growth adversely.  (EL)
305. Goyal, S. M., and C. P. Gerba.  1979.  Comparative Adsorption of Human
        Entero-viruses, Simian rotarirus, and Selected Bacteriophages to
        Soils.  Applied Environmental Microbiology 38:

        Virus adsorption to soils is considered to be the most important factor
     in removing viruses after land treatment of wastewater.  Most of the stud-
     ies on virus adsorption to soils have utilized poliovirus as the model
     system.  In the present study, comparative adsorption of a number of dif-
     ferent types and strains of human enteroviruses and bacteriophages to nine
     different soil types was studied.  Under the experimental conditions of
     this study, greater than 90% of all viruses adsorbed to a sandy  loam soil
     except echovirus types 1, 12, and 29 and a Simian rotavirus (SA-11), which
     adsorbed to a considerably lower degree.  A great deal of  variability was
     observed between adsorption of different strains of echovirus type 1,
     indicating that viral adsorption of soils is highly strain  dependent.  Of
     the five phages studied, f2 and 0X174 adsorbed the least.  In addition to
     being dependent on type and strain of virus, adsorption was found to be
     influenced also by type of soil.  Thus, soils having a saturated pH of
     less than 5 were generally good adsorbers.  From these results, it appears
     that no one enterovirus or coliphage can be used as the sole model for
     determining the adsorptive behavior of viruses to soils and that
     no single soil can be used as the model for determining viral adsorptive
     capacity of all soil types.  (AA)
306. Graetz, D.  1976.  Dynamics of Sediments in the Cypress Domes.  In H. T.
        Odum, K. C. Ewel, J. W. Ordway, and M. K. Johnston  (eds.), Cypress
        Wetlands for Water Management, Recycling, and Conservation.  Third
        Annual Report.  Center for Wetlands.  Univ. of Florida.  Gainesville.
307. Graetz, D. A.  1980.  Denitrification in Wetlands as a Means of Water
        Quality Improvement.  Rept. No. 48.  Univ. of Florida, Gainesville.
        75 pp.

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308. Graetz, D. A.., P. A. Krottje, N. L. Erickson, J. G. A. Fiskell, and D. F.
        Rothwell.  1980.  Denitrification in Wetlands as a Means of Water
        Quality Improvement.  Publication 48.  Water Resources Research
        Center, Univ. of Florida, Gainesville.   86 pp.

        The feasibility of using natural wetlands to remove nitrogen via
     denitrification from secondarily treated municipal sewage effluent was
     studied. Nitrification and denitrification rates were measured in simu-
     lated marsh ecosystems, i.e., soilrwater columns containing approximate-
     ly 30 cm of marsh soil and either 15 or 30 cm of overlying water (secon-
     darily treated sewage effluent).  Nitrification was apparently rate
     limiting when nitrogen was supplied in the form of ammonium.  Therefore,
     efficiency of nitrogen removal would be increased by allowing the nitrogen
     in wastewater to nitrify prior to entering the marsh.  First-order denit-
     rification was observed in soil:water columns.  Average nitrate removal
     rates, assuming a floodwater nitrate concentration of 10 mg N liter"^,
     were 1.2 kg N ha~1 without plants and 2.0 kg N ha"1day"1 with plants.
        Denitrification potentials of Florida wetland soils were determined
     using 15 soils with a wide range of texture, organic matter content, and
     soil reaction (pH).  Denitrification followed apparent first-order
     kinetics, with rate constants varying from 0.040 day"1 to 0.192 day"1.
     Nitrate removal rates, assuming a 10 mg N liter"1 floodwater nitrate con-
     centration, ranged from 0.6 to 2.9 kg N ha~1day~1. Denitrification rate
     was significantly correlated with soil organic content and with soil pH.
     Nitrous oxide accounted for from less than 0.2 to 6.5% of nitrate con-
     sumed.
        The experimental data obtained in this work indicate that many Florida
     wetlands could function as efficient treatment systems for nitrate-bearing
     wastewater and drainage water.  Soils high in organic matter with a pH
     range of 5 to 7 would provide the most rapid removal rates. (AA)
309. Graetz, D.A, and J. Struble.  1975.  Rates of Sedimentation: Sediments as
        a Nutrients Sink in Cypress Domes, pp. 561-563.  In; H.T. Odum, K.C.
        Ewel, J.W. Ordway, and M.K. Johnston (eds.), Cypress Wetlands for Waste
        Management, Recycling, and Conservation. Third Annual Report.  Center
        for Wetlands. Univ. of Florida.  Gainesville.
310.  Grant, R.  R., Jr., and R. Patrick.  1970.  Tinicum Marsh as a Water
        Purifier,   pp. 105-123.  In J. McCormick, R. R. Grant, and R. Patrick
        (eds.).  Two Studies of Tinicum Marsh, Delaware and Philadelphia
        Counties,  Pennsylvania.  The Conservation Foundation, Washington, DC.

        The general health of a marsh receiving water from a creek polluted by
     municipal  sewage was studied in an attempt to assess the effectiveness of
     marshes in improving water quality.  Results indicated that marsh vegeta-
     tion and algae help reoxygenate water, reduce nitrates and phosphates, and
     generally  enhance water quality.

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 311.  Grasso,  S.  V.   1979.   An Analysis of the Factors Affecting the Distribu-
         tion  of  Heavy Metals in a Tidal Estuary.   Ph.D.  Diss.   Rutgers Univ.,
         New Brunswick, NJ.   288 pp.
 312.  Greenwald,  C.  M.   1981.   Prediction of Songbird Responses to Habitat
         Alteration  Resulting from Wastewater Irrigation.   School of Forest
         Resources.   Pennsylvania State Univ., University Park.  81 pp.

         In an effort to predict songbird responses to habitat changes result-
      ing from year-round spray irrigation of chlorinated municipal wastewater,
      relationships  between habitat features and attributes of songbird popula-
      tions and communities were studied on Penn State's Wastewater Renovation
      Facility.   Habitat types in the study area ranged from shrubby old fields
      to mixed-oak forest.   Songbirds were censused at 30 randomly chosen
      points four times between June 1979 and May 1980.  Principal components
      analysis of the habitat measurements resulted in a reduction of the 34
      original variables to nine habitat components.   Multiple regression of
      avian community parameters in each season on the habitat components
      produced ten signficiant equations; fall and winter N could not be pre-
      dicted.
 313.  Greer,  D.  E.,  and C.  D. Ziebell.   1972.   Biological Removal of Phosphates
         from Water.   J.  Water Pollut.  Control Fed.   44:2342-2348.

         Various organisms  were tested for their ability to remove the ortho-
      phosphate  ion  from solution.   A system incorporating natural algal succes-
      sions and  beds of the oriental clam Corbicula fluminea Muller was the most
      effective  of  the systems tested.   Experiments were conducted under con-
      trolled conditions at concentrations of  5.0, 10.0, and 15.0 mg/1 PO3.
      Results indicated that this system can remove the PO3 ion to below 0.10
      mg/1 in 16 days or less and yield a clear effluent.  (AA)
 314.  Greeson,  P.E.,  J.R.  Clark,  and J.E.  Clark (eds.). 1979.  Wetland Functions
         and Values:  The State of Our Understanding,  Proceedings of the National
         Symposium on Wetlands, Lake Buena Vista, Florida. Amer. Water Resour.
         Tech.  Publ.  TPS 79-2.  Minneapolis,  MN.  674 pp.
 315.  Greij,  E.  D.   1976.   The Effects of a Marsh on Water Quality.  Project
         Completion Report.   Michigan Inst. of Water Res., Michigan State Uni.
         Lansing.
1073.  Grimes,  D.   In press.
 316.  Gross,  F.,  and G.  Ryvarden.  1981.   Effluent Treatment Systems Requiring
         No External Chemicals or Energy  Supply for Use in Protected Groundwater
         Zones in the Chalk Uplands.   Model Studies.  Gas, Wasster, Warme,
         (Austria) 35(2):49-52.

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        Problems of sewage disposal in mountain refuges in protected  areas
     used for groundwater supply are considered.  Experiments are described  in
     which a rudimentary biological filter was connected in series with  a
     number of shallow containers forming a cascade system for aquaculture of
     phytoplankton and higher forms of plant life.  The containers were
     arranged so that they experienced direct sunlight, and the system was fed
     with effluent from a septic tank.  Progressive elimination of dissolved
     organic matter and ammonia occurred with a steady decrease of coliform
     counts through the action of the algae and the sedge grass in the basins;
     the possibility that antiobiotics are released by these grasses  is  con-
     sidered to merit further study.  By using a sufficient number of basins
     in series, effluent approaching the quality of raw water sources for
     potable supplies should be obtained.  Methods of composting the  sludge
     from the septic tank in a mixture with straw and quicklime were  also
     tentatively investigated.  (AL)
317. Guntenspergen, G., and F. Stearns.  1979.  Ecology of an Ombrotrophic Bog in
        Northern Wisconsin.  Bull. Eco. Soc. of Americal  60:135.

        This study was designed to provide background information  on plant nutri-
     ent content, primary productivity and biomass in an ombrotrophic bog.   Such
     basic knowledge is lacking for ombrotrophic peatlands in North America.  Our
     work concentrated on the herb and shurb layers and is part of a larger  re-
     search effort investigating the biological and physiochemical nature of  this
     community.
        The study site is located in northeastern Wisconsin thirty miles south of
     Lake Superior in Bayfield County.  The area is physiographically complex
     with calcareous maraines and wet depressions creating a diverse landscape.
     Drummond bog is a 22 acre perched bog system isolated from the influence of
     the regional ground water table.  The mean pH is 4.5.  Runoff from the  up-
     land is collected in the moat or lag zone around the bog.
318. Guntenspergen, G., W. Kappel, and F. Stearns. 1980.  Response  of  a Bog to
        Application of Lagoon Sewage: The Drummond Project - An Operational
        Trial.  Presented at the 6th International Peat Congress. Duluth,  MN.
        Aug. 17-23.  8 pp. plus attachments.

        The town of Drummond, in northwestern Wisconsin, completed  a three-
     lagoon, secondary sewage treatment system in 1978.  To avoid discharging
     the effluent into a Class 1-A trout stream, the U.S. Forest Service sug-
     gested a tertiary treatment alternative where the lagoon effluent passes
     through a 10 ha. bog.
        Pretreatment data on water quality of the bog outflow, and  productivity
     and nutrient content of the bog vegetation is typical for bogs of this
     type.  Wastewater from the lagoons was applied to the bog during  the  1979
     growing season. Preliminary water quality analysis indicates that the  bog
     is assimilating the nutrients in the effluent although the total  loadings
     were relatively small.  Plant tissue nutrient concentrations changed  less
     than those in the surface horizons of the past.
        Parameters other than nutrient immobilization require attention before
     the widespread use of such systems becomes common.  The long term response
     of peatland ecosystems to sewage effluent disposal must be assessed.   (AA)

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 319.  Guntenspergen,  G.,  and F.  Stearns.   1981.   Ecological Limitations on Wet-
         land Use  for Wastewater Treatment,  pp.  273-284.  In; B. Richardson
         (ed.),  Selected Proceedings of the Midwest Conference on Wetland Values
         and Management.   Minnesota Water Planning Board.  St. Paul.  660 pp.

         Wetland use  for tertiary treatment of sewage effluent is gaining wide-
      spread attention partly for economic reasons and partly because wetlands
      are presumed to absorb nutrients and retain them.  At one time, surface
      waters  were assumed to have an infinite capacity to absorb nutrients.
      The ecological  consequences of that assumption were streams and lakes rich
      in phosphorus and nitrogen filled with blue green algae and nuisance
      plants; a  condition called cultural eutrophication.   Conventional sewage
      treatment  is effective in removing organic material but not nutrients.
      Advanced wastewater treatment removes nutrients, but its cost is often
      prohibitive  for small communities.   These small communities need alterna-
      tive techniques to treat their sewage and meet water quality standards.
         What happens when nutrients are transferred from an effluent flow to a
      wetland?  The ultimate result is still difficult to predict; we have an
      imprecise  understanding of nutrient cycling at the ecosystem level.
      Traditionally the environment is viewed as an enormous sink and the
      usefulness of an ecosystem is measured by the amount of human waste it
      can absorb.  Freshwater wetlands have long been thought to improve the
      quality of waters flowing through them.  It was inevitable that these
      systems would be perceived as being capable of treating wastewater.
         Two lines of inquiry are needed to evaluate this wetland alternative
      for waste  disposal.  First, we must determine the ability of wetland
      ecosystems to accommodate nutrient inputs, i.e., the effectiveness of the
      process.  Second, we must examine the ecological consequences — i.e., the
      wetland community response to large nutrient inputs.
1074.  Guntenspergen,  G.R.  and F. Stearns.  In press.


 320.  Gupta,  G.C.,  1980.   Use of Water Hyacinths in Wastewater Treatment.  Jour-
         nal of Environmental Health.  43(2):80-82

         A literature review is presented on the use of water hyacinths in
      sewage and wastewater treatment.  Water hyacinths have been effective in
      removing algae, fecal coliforms, suspended particles, trace toxic metals,
      organics, and many other dissolved impurities from wastewater.  Water hya-
      cinths are classified as an aquatic pest, but culturing hyacinths in a
      sewage lagoon system can reduce 8005 up to 95%, total suspended solids
      up to 90%,, and also reduce nitrogen, phosphorus, heavy metals, and pesti-
      cides.   Biological,  chemical, and physical factors combine to  remove con-
      tamination.  Five and one-tenth acres of water hyacinths are needed for 1
      mgd throughout to remove 80% of the nitrogen and 44% of the phosphorus.
      Hyacinths are effective and cheap where land is available and  inexpensive.


 321.  Gupta, P.K.  1977.   Dynamic Optimization Applied to Systems with Periodic
         Disturbances.  Ph.D. Dissertation Univ. of Michigan, Ann Arbor.


 322.  Haag, R.D., Jr.  1979.  The Hydrogeology of the Houghton Wetland.  Ph.D.
         Dissertation  University of Michigan, Ann Arbor.

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323. Hagarty, E. P.  1978.  Wastewater Disposal on Wetlands.  North  Carolina.
        Department of Natural Resources and Community Development, Division  of
        Environmental Management.  Raleigh, NC, 18 pp.

        This report contains an overview of some of the wetland  treatment
     system projects in the United States and the advantages, disadvantages,
     and uncertainties of such techniques as part of an analysis of  the poten-
     tial for the use of this type of treatment system in the coastal  areas  of
     North Carolina.  The author recommends establishing a pilot study in
     North Carolina and  identifies the general types of wetlands found there*
     (EPA)
324. Haines, E.B., 1974.  Growth Dynamics of Cordgrass, Spartina alterniflora
        Loisel on Control and Sewage Sludge Fertilized Plots in a Georgia  Salt
        Marsh.  Estuaries 2(1):50-53.

        Seasonal plant growth dynamics were followed for a year in  undisturbed
     plots of tall and short form Spartina alterniflora and in plots of short
     form S_. alterniflpra which were enriched with  sewage sludge.   Monthly de-
     terminations of above ground live and dead biomass, density of live stems,
     the ratio of number of young shoots to total number of shoots, and below
     ground mass of macro-organic matter to a depth of 30 cm were made for each
     area.  Sludge fertilization increased the live biomass of the  short form
     S_. alterniflora by up to 150% of the control live biomass, but had little
     effect on the dead biomass, stem density, or proportion of young shoots.
     There was a trend of increased amount of below ground macroorganic matter
     in fertilized compared to control plots during the last 6 months of the
     study.  In all areas, there was a marked decrease in the proportion of
     young shoots from winter to early summer, followed by a rapid  increase in
     the percent of young shoots from late summer to fall.  Sampling of plots
     7 and 20 months after termination of sludge enrichment showed  higher  plant
     biomass and % N content in surface soils, but no difference in N content
     or live plant tissue, in fertilized compared to unfertilized marsh.   After
     20 months, about half of the sludge nitrogen remaining in the  soils of the
     fertilized plots had disappeared.
325. Haines, E.  1980.  Salt Marsh Creation: Impact of Sewage. In: J.C. Lewis
        and E.W. Bruce (eds.), Rehabilitation and Creation of Selected Coastal
        Habitats: Proceedings of a workshop. (Rept. No. 80/27).  Office of  Bio-
        logical Services.  U.S. Pish and Wildlife Service.

        A study of the capacity of a Spartina salt marsh to assimilate nitrogen
     from sewage was carried out in Georgia.  Focus has been on the basic
     nitrogen cycle as well as the impact of applying sewage sludge to the
     marsh;  specifically what happens to the nitrogen in the sludge.
        Coastal urban areas have found disposing of raw sewage in coastal wet-
     lands inexpensive and convenient.  The increasing BOD has already resulted
     in deterioration of water quality in some northern estuaries.  The pro-
     cesses  in the estuaries tend to keep materials that come into estuaries
     within  the estuary.  Major components of sewage are plant nutrients, heavy
     metals, chemicals, pesticides, petroleum hydrocarbons, and pathogenic

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     microorganisms.  Evaluation of sewage impact is based on 1) accumulation
     of substances, 2) stimulation of biological processes, and 3) inhibition
     of microbial processes.
        Salt marshes are not effective for secondary sewage treatment. However,
     estuaries may be efficient at tertiary treatment.
326. Hall, F., R. Rutherford, and G. Byers. 1972.  The Influence of a New Eng-
        land Wetland on Water Quantity and Quality.  Research Report No. 4.
        Water Resources Research Center.  Univ. of New Hampshire, Durham, H.H.
327. Hallock, R.J., and C.D. Ziegell.  1970.  Feasibility of a Sport Fishery in
        Tertiary Treated Wastewater. J. Water Pollut. Control Fed. 42:1656-1665.

        The objective of this study was to test the feasibility of developing a
     sport fishery in tertiary treated domestic wastewater.  Fish tested were
     channel cat fish, Ictalurus punctatus, rainbow trout, Salmo gairdneri, and
     Malacea Tilapia hybrids.  Less than 1 percent survival occurred in five
     trout experiments and in one catfish experiment. An important cause of
     death was low oxygen sunrise tensions.  This seemed to be a result of
     respiration by dense phytoplankton blooms, which were stimulated in part
     by high (14 mg/1) average inflow orthophosphate concentrations, and oxy-
     gen reduction through bacterial respiration.  When fish survived, produc-
     tion was high. The highest combined yield of locally acceptable channel
     catfish and Tilapia from one pond was 697 Ib/acre (779 kg/ha), a yield
     much greater than that from natural waters.  Chironomus larvae, the
     predominant food organism, comprised 90 percent of the estimated annual
     benthic production of 12.680 Ib/yr/acre (14,180 kg/yr/ha).  Phytoplankton
     productivity averaged 10.2 g O2/day/cu m.  These tertiary treated
     waters, although highly productive, need more stable oxygen conditions to
     establish dependable fishery.   (AA).
328. Hamdy, M. K.  1977.  Biochemical Transformation and Detoxification  of
        Mercury in Aquatic Environments. Rept. No. B-069-GA.  Environmental
        Research Center.  Georgia Institute of Technology.  Atlanta.   85 pp.
329. Hammer, D. E., and R. H. Kadlec.   [No  date.]  Wetland Utilization for
        Management of Community Wastewater  - Concepts  and Operations  in
        Michigan.  Industrial Development Division,  Institute  of  Science  and
        Technology, Univ. of Michigan,  Ann  Arbor.  28  pp.

        This booklet contains a summary of  the  results of the  operation of
     four wetland  irrigation systems  in Michigan  (Bellaire,  Houghton  Lake,
     Kinross,  and  Vermontvi1le).   Basic concepts  of  the processes that use
     wetlands  for  advanced wastewater treatment are  explained  in  easily under-
     standable terms and diagrams.  Implementation factors  such as economics,
     site selection, design criteria, and monitoring procedures are discussed.
     (EPA)

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 330.  Hammer,  D.  E.,  and R. H. Kadlec.  1980.  Orthophosphate Adsorption on
         Peat Presented at the 6th International Peat Congress. Duluth, MN,
         Aug.  17-23,  1980.  19 pp.


 331.  Hammer,  D.E.,  and R.H. Kadlec.  1982.  (Preliminary Draft). Design Princi-
         ples  for Wetland Treatment Systems.  Work conducted under cooperative
         agreement between U.S. EPA, Robert S. Kerr Laboratory, Office of Re-
         search and Development, Ada OK, and the University of Michigan, Ann
         Arbor, MI.
1075.  Hammer, D.E.  and R.H. Kadlec.  1983.

1076.  Hammer, D.E.  et al.  1983.


 332.  Handley, L. L.  Effluent Irrigation of a Tropical Wetland Forage Grass in
         Hawaii.   Presented at the Water Reuse Symposium II.  Washington, DC.
         August 23-28, 1981.

         California grass (Brachiaria mutica Forsk.) Stapf  (Panicum purpuras
      cens Raddi) irrigated with domestic sewage effluent shows
      excellent results as a means for water use, nitrogen  removal and high
      productivity of medium grade fodder.  This grass tolerates prolonged
      flooding and has a high transpiration rate.  It has a broad geographic
      potential for use, as its range includes a large portion of the tropics
      and sub-tropics.  In Hawaii it is used both as a pasturage for cattle and
      horses and for fodder for dairy cattle.  The grass is allelopathic and
      forms a dense, easily maintained monoculture which is tolerant to C1~ and
      Na+.
         California grass in eight percolate-style lysimeters was irrigated
      with sewage effluent, varying the volume and concentration of effluent.
      Irrigation water, soil percolate, and plant tissues were analyzed for
      nitrate/nitrite-nitrogen and ammonia plus organic nitrogen.  Soil was
      analyzed for ammonia and mineralizable nitrogen.  A water budget was kept
      and a mass  balance for nitrogen and water was calculated.
         At the highest irrigation levels (89 mm/week) percolate NO~3-nitro-
      gen did not exceed 10 ppm, nor did nitrate-nitrogen accumulate to toxic
      levels in the grass.
         Plant productivity was up to 156 mT/ha/year, dry weight.  Irrigation of
      more than 76 mm/week decreased productivity slightly.  Growth was also
      slightly depressed during lower winter temperatures.
         Water use, as evapotranspiration, was about half of that of an adjacent
      Class A pan.   Heavy irrigation and large water use are advantages in
      Hawaii where human population is dense and land is expensive and scarce.
      (AA)
 333.  Hanes, N. B.,  L. C. Brown, M. S. Bartlett, and N. H. Nickerson.   1979.
         Denitrification in Freshwater Wetland Soil.  J. Env. Qual. 8(4):
         460-464.

         The application of secondary-treated wastewater to freshwater wetlands
      as a method of tertiary treatment has been studied.  Nitrate removal in
      wetland soil-water suspensions was studied using a Warburg respirometer.
      All important  pathways of nitrate reduction were accounted for using a

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     nitrogen mass balance.  Results show that 90 to 95% of the nitrate added
     to wetland soil-water suspensions is reduced to nitrogenous gasses, with
     little or no transfer of nitrate to ammonia nitrogen or organic nitrogen
     fractions.  (AL)
334. Hansen, R. J., and J. W. DeWitt.  1967.  A Study of Some Physical and
        Chemical Environmental Features of a Large Sewage Oxidation Pond.
        Part I.  Final Report Humboldt State College, Arcata, CA.  133 pp.

        The specific objective of this study was to gain background informa-
     tion concerning certain chemical and physical features of the Arcata, CA,
     sewage oxidation pond in order to assess waste treatment performance of
     the pond relative to future operational changes.  Secondary objectives of
     the study were to evaluate present waste treatment performance of the
     Arcata pond and to determine its ability to support fish life.   (NT)
335. Hanseter, R.  1975.  Recovery, Productivity and Phosphorus Content of
        Selected Marsh Plants After Repeated Cuttings.  Master's Thesis, Univ.
        Wisconsin, Oshkosh.  81 pp.
336. Hanson, R.B. 1977. Nitrogen Fixation  (Acetylene Reduction) in a Salt Marsh
        Amended with Sewage Sludge and Organic Carbon and Nitrogen Compounds.
        Applied and Environmental Microbiology 33(4):846-852.

        Results of a 12-month study on nitrogen fixation in a  sewage sludge-
     amended Spartina alterniflpra salt marsh were reported.   Four 100-meter
     plots were used, two of these treated with sludge to the  equivalent of 4
     grams of nitrogen per square meter.   The effects of nutrient amendment
     were also studied on similar plots, divided  into 10 treatment areas.  Half
     of these areas were clipped of S. alterniflpra  and enclosed in lawn
     edging to prevent lateral root movement.  Clipped and undipped plots were
     injected with glucose, ammonium nitrate, a combination of the two, rho-
     damine WD, and distilled water as a control.  The acetylene reduction
     method was used to measure nitrogen fixation.   No significant difference
     was found between the sludge-amended  plots and  the controls.  Apparently
     sludge enhanced rhizosphere nitrogen  fixation in the soil through stimula-
     tion of Spartina production.  Other processes,  however,  such as denitrifi-
     cation, may be inhibited by sludge treatment. In the clipped and  undipped
     plots, nitrogen fixation was significantly lower in the  clipped plots due
     to the lack of carbon input via the roots.   Nitrogen fixation in  the
     ammonium nitrate-enriched undipped plot was increased,  indicating that
     increased Spartina production from nitrogen  addition out-weighed  the
     inhibitory effect of inorganic nitrogen on nitrogen fixation.  Glucose
     addition inhibited nitrogen fixation  in the  soil, perhaps due to  its  stim-
     ulation of microbial activity.

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337. Hantzche, N.N. 1983.  Wetland Systems  for Wastewater Treatment:  Engineer-
        ing Applications  (draft manuscript).  In; Ecological Considerations  in
        Wetlands Treatment of Municipal Wastewaters.  Proceedings  of  a Workshop
        23-25 June 1982, University of Massachusetts, Amherst MA.  US  Fish  and
        Wildlife Service and US Environmental Protection Agency.
338. Harding, J.P.C., and B.A. Whitton.   1978.  Zinc, Cadmium, and Lead  in
        Water, Sediments, and Submerged Plants of  the Derwent Reservoir,
        Northern England.  Wat. Res.  12:307-316.

        A partial budget is presented of  the  zinc, cadmium and lead entering
     the Derwent Reservoir.  The mean levels  in the water column  upstream of
     the site of inflow are Zn. 0.216 mg/1; Cd. 0.003 mg/1; Pb. 0.065 mg/1;
     levels after passage through the 4.1 km^ reservoir  fall by:  Zn. 70.3%;
     Cd. 98.3%; Pb. 89.2%.  Most of these metals are deposited in sediments,
     the mean values for which are; Zn. 1035 micrograms/gram; Cd.  13 micro-
     grams/gram; Pb. 827 micrograms/gram.  Lead, a higher percentage of which
     occurs as particulate material,  is deposited more rapidly than zinc;  this
     effect is especially obvious when streaming of cold water along the bottom
     of the reservoir takes place at  the  time of floods.  Macroscopic plants
     are only occasional in this reservoir, due perhaps  in part to  heavy metal
     toxicity.  Of the two most common submerged species, Nitella flexilis
     probably accumulates almost all  of its metal content directly  from the
     water, but the data suggests that sediments are a source of  some of the
     heavy metals accumulated by Glyceria fluitans.  (AA).
339. Harlin, M.M., 1981.  Nitrate Uptake by Enteromqrgha spp.  (Chlorophyceae):
        Applications to Aquaculture Systems.  Aquaculture.  15(4):373-376.

        Enteromorpha linza. and E. intestinalis removed nitrates  from a  sea
     water test medium at rates considerably higher than those measured for
     other seaweeds.  At 15 C these algae showed a V-max of 129 micro mol/h/g
     dry weight and a Ks of 17 micro M.  At similar temperature and irradiance,
     Vmax and Ks for nitrates in Enteromprpha species are twice that of Codium
     fragile.  In aguaculture systems where the objective is to strip nitrates
     from sea water, nitrate-rich water may be pulsed through tanks of Entero-
     mprpha species during the day.  Night feeding of Chondrus crispus culture
     tanks would capitalize upon the difference in nitrate uptake strategies
     between these two algae minimizing uptake by the Enteromprpha.  This paper
     investigates three parameters that regulate nitrate uptake for Enteromgr-
     pjhia linza and E.  intestinalis; temperature, lights, and substrate concen-
     trations.  At five C nitrate uptake was 19% of that measured at 15 C and
     at 0 C nitrate uptake was not detected.  In darkness, nitrate uptake was
     reduced 35% in the first hour, 10% in the second hour.  After standing in
     nitrate-depleted sea water, algae scavenged nitrate at concentrations 0.5
     micro M and no nitrate leaked from the tissue into nitrate-free water.
     Nitrate uptake increased with increasing substrate concentrations.
340. Harmston, F.C., and L.J. Ogden.  1976. Mosquito Problems Associated with
        Man-made Impoundments in Western and Mid-western United States.

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341. Harris, S.W. , and W.H. Marshall.  1963.  Ecology of Water-level Manipula-
        tions on a Northern Marsh.  Ecology 44(2):331-343.

        A study of vegetarian changes associated with marsh drawdowns at
     Agassiz National Wildlife Refuge, Minnesota, revealed that the development
     of five types of vegetation on mud flats during the first year was influ-
     enced by seed availability, soil type and moisture, season and duration of
     drawdown, and the amount of stranded algal debris.  The more an area com-
     bined early season drawdown, rich soil types, slow rates of mud flat
     drainage, and small amounts of stranded algae, the greater was the devel-
     opment of emergent aquatics.
        In the second year of drawdowns, most areas developed greater amounts
     of upland and shoreline weeds and fewer emergents.  Areas originally
     exposed before August of the first year lost emergent cover during the
     second year, while the reverse was true of areas exposed later in the
     first year.  Specific changes were influenced by density and comosition of
     residual vegetation, soil types, and soil moisture.  During longer draw-
     downs the soil dried more completely, and over a 5-year period nearly
     solid stands of willow developed.
        Upon reflooding, mud flat and shoreline annuals were eliminated and
     marshes of cattails, soft-stem bulrush, sedges, spike-rush, willows, and
     aquatic annuals developed in the first year.  Specific development in sub-
     sequent years was determined by the nature of the residual vegetarian and
     the depth of the restored water.  (AA).
342. Barter, R.D. 1966.  The Effect of Water Levels on Soil Chemistry  and Plant
        Growth of the Magee Marsh Wildlife Area.  Ohio Game Mono. No.  2.  Ohio
        Div. of Wildlife, Columbus. 36 pp.
343. Hartland-Rowe, R. C. B., and P. B. Wright.   1974a. Effects  of  Sewage
        Effluent on a Swampland Stream, pp.  1575-1584.  In V.  Sladecek (ed.),
        Proceedings of the International Association of Theoretical and
        Applied Limnology (Verb. Int. Ver. Limnol).

        Wastewater was introduced into a riverine  wetland near Hay  River,
     Northwest Territories, Canada.  A variety of  water quality  parameters
     were studied.  Dramatic improvements were shown observed  for many par-
     ameters, including total phosphorus and suspended solids.   (EP)
344. Hartland-Rowe, R. C. B., and P. B. Wright.   1974b.  Swamplands for Sewage
        Effluents.  Final Report.  Prepared  for  the  Environmental-Social
        Program, Northern Pipelines, of the  Task Force on Northern Oil Devel-
        opment.  Information Canada.  Ottawa,  Ontario, Canada,  137 pp.

        This is the final report of the two-year study described in Hart-
     land-Rowe and Wright (1974a).  The creek  flows  intermittently into Great
     Slave Lake and broadens in a number  of  locations into  pools bordered with
     Carex.  Some standing  crop measurements and phosphate  analyses of
     sediment cores and  root masses were  made  in addition to the primary
     research on water quality.  (EP)

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345. Harvey, R. M., and J. L. Fox.  1973.  Nutrient Removal Using Lemna minor.
        J. Water Pollut. Control Fed. 45(9):1927-1938.

        Wastewater nutrient removal capabilities and economic resource poten-
     tial of Lemna minor are investigated and discussed.  L. minor was selected
     over Wqffia Columbiana and Salvinia rotundifIpra as the priincipal subject
     of the investigation because it was the only one of the three species
     screened that was both easy to harvest and has a high forage potential.
     Lemna removed substantial quantities of nitrogen and phosphorus from sec-
     ondary effluent under batch laboratory conditions.  Dried Lemna plants
     contained 4.59% nitrogen and 0.80% phosphorus.  Lemna was found to double
     its area every 4 days and is as good or better than common pasture grass
     as a cattle feed based on digestability testing.  Furthermore, harvesting
     costs are low because of its small size and free floating nature. (AA)
346. Haughey, A. W. V.  1968.  The Planktonic Algae of Auckland Sewage Treat-
        ment Ponds.  New Zealand J. of Marine and Fresh Water Resources 2:
        721-766.
347. Haughey, A. W. V.  1969.  Further Planktonic Algae of Auckland Sewage
        Treatment Ponds.  New Zealand J. of Marine and Freshwater Resources
        3:245-261.
348. Haung, C. P., H. A. Elliott, and R. Ashmead.   1977.  Interfacial Re-
        actions and the Fate of Heavy Metals in Soil-water System.  J. Water
        Pollut. Control Fed.  49:745-756.

        The fate of heavy metals in soil-water systems is primarily controlled
     by the congruent-incongruent solubility of the carbonates, oxides,  sul-
     fates, and sulfides of metals and by the adsorptions of free and complexed
     metals at solids-solution interface.  The presence of organic substances
     modifies the overall interfacial reactions.  Adsorption of copper,  zinc,
     lead, and cadmium increased abruptly at a give pH when the free metal  ion-
     ic species dominates the system.  At low pH, lead and cadmium were  nega-
     tively adsorbed, which results in an excess of lead and cadmium ions in
     the bulk solution phase.  Copper and zinc were always adsorbed by all  four
     solids (SiO2, AL2C>3, Evesboro, and Metapeak) investigated.  In a
     neutral to acidic environment, the adsorption  reaction appears to be the
     most important process in controlling the chemical makeup of the soil
     water system.  (AA)
349. Have, M. R.  1973.  Effects of Migratory Waterfowl on Water Quality  at
        the Montezuma National Wildlife Refuge, Senec County, New York.   J.
        Research 1(6).

        Studies were conducted in response to shellfish industry's  concern
     that bacteria effluent from northeastern wildlife refuges were adversely

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     affecting shellfish.  Results showed that the net effect of the  refuge  is
     to improve the quality of the water which flows through it.
350. Heimburg, K.F.  1976a.  Hydrology of Some North-Central Florida Cypress
        Domes.  Master's Thesis, Department of Environmental Engineering
        Sciences, University of Florida, Gainesville. 115 pp.
351. Heimburg, K.F.  1976b.  Hydrology and Water Budgets of Cypress Domes,
        pp. 56-67.  In H. T. Odum, K. C. Ewel, J. W. Ordway, and M. K. Johnson
        (eds.), Cypress Wetlands for Water Management, Recycling and  Conser-
        vation.  Third Annual Report. Center for Wetlands, Univ. of Florida,
        Gainesville.

        Intensive hydrological measurements in two cypress domes near Gaines-
     ville, FL, were conducted between 30 May 1975 and 30 April 1976.  This
     report is a summation of findings detailed in Heimburg  (1976a).
352. Heimburg, K.  1977.  Use of Florida Cypress Domes as Tertiary  Treatment
        Facilities, pp. 165-177.  In C. B. DeWitt  and E. Soloway  (eds.),
        Wetlands Ecology, Values, and Impacts.  Proceedings  of  the  Waubesa
        Conference on Wetlands held at Madison, Wisconsin, 2-5  June 1977.
        Institute for Environmental Studies, Univ. of Wisconsin,  Madison.

        Cypress domes can function as tertiary treatment facilities.   Long
     range questions about the accumulation of nutrients in  the sediments  and
     the importance of any virus that may  escape to  the water table aquifer do
     remain, however.
        Because the wastewater load is limited by  wet seasons to  something
     under an inch per week it seems that  this treatment alternative  would be
     most attractive in small scale applications,  such as the trailer park
     that produces the effluent used in the experiment.  In  addition,  small
     developments like this are least likely to be able to afford tertiary
     treatment.  Ordway  (1976) has made cost estimates for this disposal  tech-
     nique that show it to be competitive, even considering  land  and  piping
     costs, factors not usually considered in treatment cost estimates.   Boyle
     Engineering, Inc., of Orlando is currently involved in  a study comparing
     the cypress dome treatment method with others with which they  have  exten-
     sive experience.
        Although the final word will not be in for some time, tertiary treat-
     ment via cypress swamps does look like a technically and economically
     feasible way to meet 1983 water quality standards while helping  to
     recharge deeper aquifers.   (AA).
353. Heimbuch, D. G.,  S. Perretta,  and J.  G.  Nickum.   1978.   Cultured Daphnids
        as a Biological Wastewater  Treatment  System for Oxidation Pond Effluent
        Clarification.  Tech.  Completion Rept.  Office of Water Research and
        Technology, Washington, D.C.  68 pp.

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         The technical feasibility of culturing Daphnia magna in  flowing water
      and their function as biological filters for removing algae  from the
      effluent of simulated oxidation ponds was demonstrated.  Greatest daily
      increases in daphnid population biomass in 5.5 liter culture vessels were
      observed in populations receiving flows of low algal concentration
      (3 mg/1) and high flow rate (240 ml/min).  The maximum four  day average
      increase for three replicate populations of that treatment was 136 mg
      (dry weight)/liter/day.  Water leaving the culturing vessels of the
      3 mg/1 x 240 ml/min treatment had significantly lower concentrations of
      total nitrogen, total phosphorus, and volatile suspended solids than
      water entering the vessels; however, control vessels with no daphnid
      populations achieved similar reductions in total nitrogen and total
      phosphorus.  Concentrations of volatile suspended solids were reduced
      significantly in vessels containing daphnids in comparison to control
      vessels.  Concentrations of ammonia nitrogen and ortho-phosphate in-
      creased significantly in passing through culture vessels containing
      daphnid populations.  (NT)
 354. Heinz, G. 1979.  MethyImercury: Reprbductive and Behavioral Effects on
         Three Generations of Mallard Ducks.  Journal of Wildlife Management
         43:394-401.
 355.  Hejkal, T.W.  1978.  The Importance of Viruses Associated with Solids in
         Water.  Ph.D.  Thesis Florida State University.
1077.  Heliotis, F.D.  1981.
 356.  Heliotis, F.D.   1982.  Wetland Systems for Wastewater Treatment: Operating
         Mechanisms and Implications for Design.  Instructional Program,
         Institute for Environmental Studies; University of Wisconsin-Madison.
         IBS Report No. 117.   68 pp.


1078.  Heliotis, F.D.  and C.B. Dewitt.  1983.


 357.  Helz,  G.  R., J.  M. Hill,  and R. J.  Huggett.  1975.  Behavior of Mn, Fe,
         Cu, Zn,  Cd,  and Pb Discharged From a Wastewater Treatment Plant into
         an  Estuarine Environment.   Water Research 9(7):631-636.

         Studies  have been carried out on the Back River, MD, a small estuary
      tributary to the Chesapeake Bay which receives effluent from the prin-
      cipal  sewage works of Baltimore, to obtain information on the fate of
      trace  metals discharged to the estuarine environment.   Except possibly
      for Mn and  Fe,  the decrease in metal concentration within 2-3 km of the
      outfall is  much greater than can be ascribed to simple dilution, and the
      metals must be  removed to the sediments.  However, after the initial
      decrease, concentrations  of Mn and Cd in the water begin to increase
      again, suggesting remobilization from the sediment.  Of the metals
      studied,  Cd poses the greatest risk because its input from sewage probably
      exceeds fluvial input,  it appears to be readily remobilized from
      sediments,  and  it is known to be toxic to many organisms.  (AL)

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358. Hemond, H., and W.  Nuttle.   1983.  Significance of Hydrology to Wetland
        Nutrient Processing (draft document).  In;  Ecological Considerations in
        Wetlands Treatment of Municipal Wastewaters.  Proceedings of a Work-
        shop, 23-25 June 1982, University of Massachusetts, Amherst MA. U.S.
        Fish and Wildlife Service and U.S. Environmental Protection Agency.
359. Henderson, C., Q.  H. Pickering, and J. M. Cohen.  1959.  The Toxicity of
        Synthetic Detergents and Soaps to Fish.  Sewage and Industrial Wastes
        31:295-306.
360. Henderson, S.  1977.  An Evaluation of Filter-feeding Fishes for Water
        Quality Improvement.  Arkansas Game and Fish Comm., Little Rock, AR
361. Henderson, S.  1979.  Utilization of Silver and Bighead Carp for Water
        Quality Improvement, pp. 309-317.  In R. K. Bastian and S. C. Reed
        (eds.), Aquaculture Systems for Wastewater Treatment: Seminar Pro-
        ceedings and Engineering Assessment.  U.S. Enviromental Protection
        Agency, Office of Water Program Operations, Municipal Construction
        Division.  Washington, DC.  EPA 430/9-80-006.

        Filter feeding fishes, the silver carp, Hypopthalmichthyes mplitrix and
     bighead carp, Aristichthyes mobilis, were stocked in an existing lagoon
     treatment system in 1975-76 for a preliminary evaluation of the effect of
     the fish on water quality and the potential of this nutrient source for
     fish production.  Positive results have led to an ongoing Environmental
     Protection Agency funded study of the efficacy of finfish as a treatment
     method in a full scale, six cell (24 acre) treatment facility at Benton,
     Arkansas.
        Information concerning water quality improvement, fish production,
     product utilization and some design considerations are presented.  The
     promising results, design adaptability, and pay back possibilities make
     this an attractive, innovative alternative.   (AA).
362. Henderson, U. B., and F. S. Wert.  1978.  Feed Fish Effluent and Reel in
        Savings.  Water and Wastes Engineering 15(6):38-39 and 43-44.

        The cost-effectiveness of aquaculture is compared with that of more
     conventional sewage-treatment methods.  Fifteen technically feasible
     strategies appropriate for an influent flow of 200,000 gal per day, and
     consisting of various combinations of activates-sludge process, bio-
     logical filters, and waste-stabilization lagoons with aquaculture race-
     ways and aquaculture fish lagoons were selected for comparison.  The
     results are tabulated and indicate that aquaculture wastewater treatments
     are viable systems, though further scientific documentation of removal
     efficiencies is  needed.  (AL)

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363. Henderson, U. B., and F. S. Wert.   1976.  Economic Assessment  of  Waste-
        water Aquaculture Treatment Systems.  U.S. Environmental  Protection
        Agency, Office of Research and Development.  EPA 600/2-76-293.
        120 pp.

        This study attempted to ascertain the economic viability  of aquaculture
     as an alternative to conventional wastewater treatment  systems for  small
     municipalities in the Southwestern  region of the United States.   A
     multiple water quality objective level cost-effectiveness model was
     employed.  A total of fifteen wastewater treatment strategies, eleven with
     aquaculture systems and four without aquaculture, were  examined.
     Estimates were made of the technical effectiveness and  the present  value
     of costs for all strategies.  Estimates of the current  value of revenues
     derived from sale of products produced in the aquaculture systems were
     made, and the impact of such revenues on total costs was analyzed.     In
     all cases, when aquaculture was deemed capable of c.chieving  a  given water
     quality objective, the aquaculture  system compared to a conventional
     system was cost-effective.  The cost differentials between aquaculture and
     conventional strategies were highly significant ranging from a low  3.8% to
     a high of 94%.  While certain data  limitations exist, especially  in the
     area of water quality estimates, aquaculture systems appear  to be low cost
     alternatives to conventional wastewater treatment systems.   (AA)
364. Hendricks, D. W., W. D. Pote, and J. G. Andrew.   1970.  A Thermodynamic
        Analysis of a Primary Waste Stabilization Pond.  Utah Water Research
        Lab.  Logan, UT.  72 pp.

        A 97.5 acre oxidation pond with an average depth of  1 1/2 to  1  2/3
     meters, in operation since 1967, was sampled in September 1969 and in
     June 1970.  The primary objective was to quantitate the actual energy
     trade-off, in terms of algae produced vs. amount  of waste degraded,  for
     oxidation ponds.  The results defined respectively: the calculated
     absolute lower limit of daily algal synthesis necessary for production of
     the stoichiometric oxygen to satisfy the daily influent BOD requirement/-
     measured daily synthesis rate of algae to compare with the daily influent
     TOC, under conditions of maximum sunshine in the  annual cycle; and the
     calculated absolute upper limit of daily algal synthesis, through  the
     annual cycle, if all usable solar energy were utilized.  (NT)
365. Henley, D.A. 1978.  An Investigation of Proposed Effluent Discharge into
        a Tropical Mangrove Estuary,  pp. 43-64.  In; Water Pollution Control
        in Developing Counties.  Proceedings of the International Conference,
        Bangkok, Thailand.  February, 1978.

        Described are environmental studies carried out to determine the likely
     effects of effluent discharge into a mangrove-fringed estuary at Darwin in
     northern Australia.  The oceanographic studies are described together with
     the statistical method used to calculate likely buildup of pollutants
     within the estuary.  Studies of the nutrient status of the mangrove, in-
     cluding their sediments, are also described.  They were used to assist in
     determining likely effects on the mangroves of an increase in nutrient. It

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     was concluded that effluent from a primary-secondary lagoon system  could
     safely be discharged within the mangrove areas with no detrimental  effect.
366. Hermann, A.J. 1980. Nitrogen Cycling in a Freshwater Marsh Receiving Sec-
        ondary Sewage Effluent.  Master's Thesis.  Department of Environmental
        Engineering Sciences.  Univ. of Florida, Gainesville.  301 pp.
367. Hermann, A., J. Zoltek, S. E. Bayley, and T. Dolan.   1979.  Nutrient
        Budget in a Fluctuating Freshwater Marsh System in Florida.  In J. C.
        Sutherland and R. H. Kadlec (eds.), Wetland Utilization for Management
        of Community Wastewater.  Abstracts of a conference held 10-12 July
        1979 at Higgins Lake, MI.

        The effect of secondarily treated municipal sewage effluent on the
     nitrogen and phosphorus budgets of a central Flordia  freshwater marsh was
     evaluated.  Four 2000 m2 experimental plots were constructed in the
     marsh. Three of the plots received effluent at controlled rates of 1.3,
     3.8, and 10.2 cm/wk, respectively.  A fourth control  plot received muni-
     cipal freshwater at a controlled rate of 3.8 cm/wk.
        Effluent and freshwater were applied to the respective plots over a
     24-hour period once each week.  Fiberglass walls were placed around the
     perimeter of each plot to a depth of 0.5 m in the peat substrate to retain
     applied water.  Screened wells were installed to the  bottom (1.5 m) of the
     peat substrate in the northwest corner of each plot for water quality
     sampling.
        Effluent and all well samples were analyzed for nitrate nitrogen,
     nittrate nitrogen, ammonium nitrogen, total Kjehldahl nitrogen, orthophos-
     phorus, and total phosphorus.  Aboveground vegetation, root, and peat
     samples were analyzed for total nitrogen and total phosphorus content.
368. Hess, A.D., F.C. Harmston, and R.O. Hayes. 1970.  Mosquito and Arbivorus
        Disease Problems of Irrigated Areas in North America, pp. 443-459. In;
        Critical Reviews in Environmental Controls (November).
369. Hickey, L.S. and P.C. Reist. 1975a.  Health Significance of Airborne
        Microorganisms From Wastewater Treatment Processes.  Part I.  Summary
        of Investigations.  J. Water Pollut. Cont. Fed. 47(12):2741-2757,  1975.

        Published field measurements and related investigations of viable  bac-
     terial aerosols generated from wastewater are summarized in detail.   Bac-
     terial aerosols of up to 1,170 viable particles/cu ft  (41,785.71/cu m)  of
     ambient air have been recovered near aerated wastewater sources.  These
     aerosols persist in much lower concentrations [about 5 viable particles/
     cu ft  (178.57 cu m)] several hundred yards downwind.   Interference from
     other aerosol sources generally is not controlled in samples taken at
     great downwind distances from the presumed source. Pathogenic genera  and
     species of bacteria are identified frequently from recovered particles  in

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     the human respirable size range.  Limited  data  indicate virulence reten-
     tion by some organisms.  Investigators  generally  infer  a respiratory
     disease hazard from experimental  results.  Viable aerosols  from land
     application of wastewater have not been examined  adequately in the field.
     However, health hazards attributed to aerosols  from this source have also
     been postulated.  Aerated wastewater also  generates protein-bearing par-
     ticulate aerosols with suggested  allergenic  significance.   No evidence of
     health effects in exposed groups  attributable to  viable aerosols has been
     reported, the single applicable health  survey being inconclusive.
370. Hickey, L.S. and P.C. Reist.  1975b.  Health Significance  of  Airborne
        Microorganisms From Wastewater Treatment Processes.  Part II.   Health
        Significance and Alternatives for Actin.  J. Water Pollut.  Control Fed.
        47(12):2758-2773, 1975.

        The air transport route for viable bacterial aerosols  from aerated
     wastewater to residential areas has been demonstrated by  field studies.
     Land application of wastewater by spraying is postulated  to  have  a much
     higher potential for production of viable aerosols  than conventional aera-
     tion.  Various field sampling protocols yield similar results,  but samp-
     lers capable of providing particle size characteristics and  volumetric
     concentrations of viable aerosols are preferred in  field  studies.   Viable
     particles recovered range in diameter from 2 to 10  microns and are predom-
     inantly in the human respirable size range.  Wind speed and  die-off from
     evaporative stress influence viable aerosol dispersion and survival.
     Selective aerosolization and survival of particular bacterial species
     reflect the need to develop a valid index agent for  monitoring viable
     aerosols.  Some evidence supports the existence of  a health  hazard from
     viable wastewater aerosols.  However, lack of evidence of actual  disease
     transmission by these aerosols divides professional  opinion  on the
     question.  Examination of susceptible exposed groups is indicated.   (AA).
371. Hickok, E.A.  1979.  Wetlands and Organic Soils for the Control  of Urban
        Stormwater.  In; Lake Restoration, EPA Report No. 440/5-79-001.

        A seven-acre wetland in the Minnehaha Creek Watershed District,
     Minnesota, was studied during 1974 through 1975 to determine  its  effect  on
     stormwater runs.   Influent and effluent water sources and amounts were
     monitored to develop water and nutrient balances.  Instrumentation used
     includes 5 parshall flumes, 13 polyvinyl chloride observation wells,  and
     a complete weather observation station.  Physical entrapment, microbial
     utilization, plant uptake, and adsorption were all found to be active
     mechanisms in the wetland system.  The phosphorus adsorptive  capacity of
     the area indicated that the organic soils of the watershed contain between
     5 and 17 times the amount of phosphorus expected.  Application of study
     results led to the development of five projects: (1) extensive environ-
     mental planning and construction was done for the building of a new shop-
     ping center in the study area; (2) a biologically activated soil  filtra-
     tion unit was constructed to filter phosphorus from the hypolimnion water
     of Wirth Lake in Minneapolis; (3) an oil retention basin, a six-acre
     wetland treatment area, and a filter dyke for drawdown were developed for

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     a watershed at the Minneapolis St. Paul airport; (4) a marsh is being
     utilized by Annandale, Minnesota, to treat combined urban runoff and
     treated  wastewater; and (5) organic soils and wetlands are being used  for
     the Long Lake Restoration Project.
372. Hickok, E.A., Hannaman, M.C. and N.C. Wenck, 1977.  Urban Runoff Treatment
        Methods.  Vol. I.  Non-Structural Wetland Treatment. Hickok  (Eugene A.)
        and Associates, Wayzata, MN.  NTIS No. PB-278  172.  EPA Rpt. No. 600/2-
        77-217.  131 p.

        The detrimental effects of stormwater runoff in the Minnehaha Creek
     Watershed District in Minnesota, leading to reduced water quality in Lake
     Minnetonka, may be alleviated by utilization to adjacent wetlands.  The
     non-structural use of wetlands for the retention of phosphorus  and sus-
     pended solids from urban runoff has been demonstrated as a feasible means
     of controlling runoff and water quality; the renovation of the  stormwater
     runoff in the wetland occurred by a combination of physical entrapment,
     microbial transformation, and biological utilization.  The wetlands stud-
     ied has a phosphorus retention capacity about 5.5 times that of soil and
     retained 77% of all phosphorus and 94% of the total suspended solids
     entering the site.  The physical entrapment of contaminants was attributed
     to the fine texture of the wetland soil, which is also responsible for re-
     ducing the velocity of the groundwater movement.  The non-structural use
     of the wetland for controlling urban runoff had no apparent impacts on the
     wildlife or vegetation in the area.
373. Higman, D., 1973.  Emergent Vascular Plants of Chesapeake Bay Wetlands
        pp. C72-C80.  In; Army Corps of Engineers, Chesapeake Bay Existing Con-
        ditions Report.  Appendix C., Vol. 2

        Two annotated checklists have recently been compiled for vascular
     plants of the Chesapeake Bay and areas potentially subject to flooding by
     it (Krauus et al. 1971; Wass. 1972).  The Maryland list of 421 species
     covers all land areas within the high tide limits of the Bay and its trib-
     utaries both in Maryland and Virginia.  The Virginia Institute of Marine
     Sciences (VIMS) list of 435 species is restricted to Virginia (including
     the Barrier Islands of the Atlantic seashore) and the Patuxent River estu-
     ary in Maryland.  Both lists include the flora of salt and brackish
     marshes, beaches, and freshwater swamps.  The VIMS also covers ponds and
     floodplains on tidal creeks and shores subject to storm wave inundation.
     Each list contains many species not found in the other, so together they
     probably comprise a fairly complete flora of Chesapeake Bay wetlands.
     Since the distribution of many salt marsh species depends to some degree
     upon salinity, engineering projects which alter the salinity of portions
     of the Bay likewise alter the floristic composition of salt marshes in the
     affected areas.  Pollution by sewage raises the level of nutrients in salt
     marshes.  Wass and Wright (1969) noted that Spartina spp. grows taller and
     has a darker green color than normal when sewage is present.  During the
     growing season, salt marshes are able to absorb some of the excess
     nutrients which otherwise would create algal blooms in the estuaries.

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 374. Hillman, W.S., and D.D. Culley, Jr.  1978.  The Uses of Duckweed.   Amer.
         Scientist 66:442-451.

         Duckweed is capable of removing organic and inorganic materials  from
      wastewater.  It is particularly effective for reducing nitrogen  and phos-
      phorus concentrations and less effective with toxic chemicals  or heavy
      metals.   Duckweed will tolerate micronutrients such as Cu,  Zn, and  B. It
      will grow in the presence of high concentrations of sodium  and will sur-
      vive in high concentrations of potassium, phenols, or other organic sub-
      stances .
         The principles and observations we have presented are not new in any
      fundamental sense, though they may be unfamiliar in combination.  The cen-
      tral concept is that of using a new crop, or group of crops, not primarily
      in monoculture but in polyculture systems having several integrated func-
      tions.  Because it is a concept not fully at ease with more traditional
      views of agriculture, aquaculture, or waste management, a good deal more
      research and development is needed to apply it successfully.   While the
      Lemnaceae,  specifically, are by no means the only plants with  promise for
      systems such as the dairy farm model described, their unique growth char-
      acteristics and worldwide distribution offer important advantages.  Current
      concern with problems of the environment and limited resources should
      direct attention to this and other novel approaches to managing  them.  (AA)
 375. Hinde Engineering Co. 1979.  Little Fish, Big Help in Sewage Treatment,
         Farm Pond Harvest 13(1):8.

         For the first time, Muskelunge, an American game fish, were  raised  in
      a sewage treatment lagoon.  The village of Dorchester, WI donated  its
      lagoons for the project that was supervised by the Wisconsin Department
      of Natural Resources.  Raising the fingerling muskies in the lagoon
      produced both high quality fish and high quality effluent from  the
      lagoon.  Minnows feed directly on natural foods in the treatment lagoons
      and muskies feed on the minnows.  A substantial portion of  the  phosphates
      were removed; 5.5 mg/1 in the influent to 2.4 mg/1 in the effluent after
      treatment in the fish lagoons.
 376.  Hodson, R. E.  1980.  Microbial Degradation of Industrial Wastes Applied
         to Freshwater Swamps and Marshes.  Kept. No. A-082-GA.  Georgia  In-
         stitute of Technology.  Atlanta.

         Methodology has been developed for assessment of the effects of  or-
      ganic industrial wastes on the natural geochemical activities  of aquatic
      microbial populations in marshes and swamps in the coastal plain of
      Georgia.   Wetlands are receiving increasing pollutional burdens due to
      the rapid industrialization of this region.  Several schemes have been
      proposed for the utilization of wetland environments as natural "tertiary
      treatment facilities" to detoxify the industrial effluents prior to their
      introduction into larger streams and estuarine ecosystems.


1079.  Hodson, E. et al.  In press.

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377. Hoeppel, R. E.  1974.  Nitrogen Transformations in Wetland Soils.  Misc.
        Paper Y-74-4.   U.S.  Array Corps of Engineers Waterways Experiment
        Station, Vicksburg,  MS.  21 pp.

        Nitrogen transformation pathways are often complex in soils that are
     periodically subjected  to submergence.  Both biochemical and chemical
     mechanisms can be implicated in these transformations.  Nitrate accumula-
     tion in the environment has caused much conern recently due to its build-
     up in animals that lack the ability to reduce it.  Young infants and many
     ruminant animals  lack nitrate-reducing enzymes, and eventually this may
     lead to respiration difficulties.  However, nitrate, or intermediates
     leading to its formation, must first be present in order to remove con-
     siderable excesses of combined nitrogen from the soil.  The report dis-
     cusses these processes  in wetlands.  (NT)
378. Holley, E. R., Jr., and D. R. F. Harleman.  1965.  Dispersion of Pol-
        lutants in Estuary Type Flows.  Report No. 74, T65-02.  Massachusetts
        Inst. of Technology.  Cambridge, MA.  202 pp.

        A review is given of the procedures which have been used for modeling
     dispersion in estuaries.  The results of this investigation may be used
     to estimate the time averaged dispersion coefficient in the equation for
     constant density portions of tidal estuaries.  (NT)
379. Hook, J.E. and T.M. Burton.  1979.  Nitrate Leaching From Sewage-irri-
        Perennials as Affected by Cutting Management.  J. Environ. Qual.
        8:496-502.
380. Hopkins, H.P., Jr.  1981.  In-situ Evaluation of the Filtering Function of
        a Piedmont Creek Swamp During High and Low Flows.  Rept. No. A-071-GA.
        Georgia Institute of Technology.  Atlanta.
381.  Horsefield, D.R. 1968.  Master Wastewater Collection and Treatment Plan
        for Bogota, Columbia.  J. Water Pollut. Control Fed. 40:1443-1458.
382. Hortenstine, C.C.  1976. Chemical Changes in the Soil Solution From a
        Spodosol Irrigated with Secondary Treated Sewage Effluent.  J. Environ.
        Qual.  5:335-338.
383. Howard-Williams, C., and W. Howard-Williams.  1978.  Nutrient Leaching
        from the Swamp Vegetation of Lake Chilwa a Shallow African Lake.
        Aquatic Botany 4(3):257-268.

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        The decomposition of Typha dpmingensis Pers. was  investigated  in Lake
     Chilwa, a shallow endorheic tropical  lake.  The decomposition  curve
     showed a typical rapid initial weight loss/ followed by  a  slowing down  of
     this process.  The amounts of various elements released  by the physical
     leaching process during the 1st rapid weight  loss period were  investi-
     gated experimentally.  A significant  proportion of the ions were  released
     from the dead Typha shoots on the  1st day of  contact with  water.   During
     this short period, loss by leaching amounted  to: Na, 26%; K, 7.5%; Ca,
     9.2%; Mg, 11.5%; P, 1.5%; and N, 0.3%.  The significance of this  rapid
     leaching process is discussed in relation to  the field conditions pre-
     vailing in the Lake Chilwa swamps, and to the general applicability of
     using swamps to remove nutrients from polluted waters.   (BA)
384. Hruder, S.E., 1981.  Water Reclamation and Reuse.  J. Water Pollut.
        Control Fed. (Literature Review Issue), 53(6):751-767.

        A literature review is presented of publications on water  reclamation
     and reuse published during 1980.  Several reviews of current  and recent
     water reclamation and reuse practices in the U.S., Japan, the Netherlands,
     and South Africa became available.  Papers on water reuse planning and
     allocation considerations included an evaluation of a series  of computer
     models for water reuse planning.  On the topic of agriculture and irriga-
     tion, several NTIS bibliographies were published, and studies dealt with
     such topics as salinity problems associated with municipal and industrial
     effluent reuse.  Several publications reported investigations of the  long-
     term effects of land irrigation with municipal wastewater.  Aquaculture
     and wetland systems received attention in publications including an
     engineering assessment of aquaculture wastewater systems.  Water reuse for
     groundwater recharge was the topic of a California symposium  and other
     papers.  The reuse of industrial water was explored for the pulp and paper
     industry, the textile industry, and organic chemicals processing; petro-
     leum, coal, and synthetic fuels processing; inorganic chemicals process-
     ing, steel mills, metal processing and finishing, recirculating cooling
     systems and power generation, and other miscellaneous processes.  Domestic
     reuse remained controversial, and health aspects of reclamation and reuse
     were considered in papers dealing primarily with bacteria and viral patho-
     gens.
385. Huggins, T. G.  1969.  Production of Channel Catfish (Ictalurus punctatus)
        in Tertiary Treatment Ponds.  Master's Thesis.  Iowa State Univ., Ames.
        120 pp.
386. Huggins, T.G.  1970.  Fish Production in Tertiary Ponds.  Southwest Water
        Works Journal 51(12):20-22.
387. Huguenin, J. E., and J. T. Kildow.  1974.  Social, Political, Regulatory,
        and Marketing Problems in Marine Saste-food Recycling Systems,

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        pp. 344-357.   In Wastewater Use in the Production of Food and Fiber.
        U.  S.  Environmental Protection Agency.  Ada, OK.
388. Huguenin, J. E. , and J. H. Ryther.  1974.  Experiences with a Marine
        Aquaculture Tertiary Sewage Treatment Complex, pp. 377-386.  In
        Wastewater Use in the Production of Food and Fiber-Proceedings.
        EPA-660/2-74-041  Environmental Technology Series.  Washington, D.C.

        Treated secondary sewage effluent has been shown to be a good complete
     fertilizer for the growth of microscopic marine plants (phytoplankton),
     which form the base of the marine food chain.  From this fact emerged the
     concept for a combined aquaculture-tertiary sewage treatment system.
     While the optimal operating points for the two objectives of nutrient
     removal and maximizing aquacultural output are not coincident, they are
     close enough to make a combined system feasible.  The paper outlines
     briefly and discusses design problems and features of such a system.  The
     essence of the concept is a treated sewage-marine phytoplankton-bivalve
     mollusk food chain.  Data and experience notes from the Woods Hole Ocean-
     ographic Institution, where such a system is presently in operation, are
     included in the report.  (NT)
389. Hunt, P.G. and C.R. Lee.  1976.  Land Treatment of Wastewater by Overland
        Flow for Improved Water Quality, pp. 151-  .  In; J. Tourbier and R.W.
        Pierson, Jr. (eds.), Biological Control of Water Pollution. Univ. of
        Pennsylvania Press, Philadelphia.

        This paper presents an overview of land treatment of wastewater, a
     somewhat detailed development of the overland flow system of treatment,
     which is probably the least understood mode of land treatment of waste-
     water; and possible modifications of current concepts of overland flow
     treatment for improved water quality.
390. Hunter, J. V., and T. A. Kotalik.   1973.  Chemical and Biological Quality
        of Treated Sewage Effluents, pp. 6-25.  In W. E. Sopper and L. T.
        Kardos  (eds.), Recycling Treated Municipal Wastewater and Sludge
        through Forest and Cropland.  Pennsyvlania State Univ. Press, Uni-
        versity Park, PA.
391. Hurtubise, F.G., 1974.  Problems of the Canadian North,  pp. 75-84.   In;
        International Symposium on Wastewater Treatment in Cold Climates.
        Environment Canada Economic and Technical Review Report. EPA 3-WP-74-3.

        The role of the Canadian Environmental Protection Service is examined.
     Projects  in the Northwest Territories  are outlined.  These include:   a
     study on  the use of  swampland lakes for sewage  treatment; the  use  of
     vacuum and recirculating toilet units  for sewage collection with subse-
     quent incineration disposal; feasibility of land disposal of sewage efflu-

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      ent or use as fertilizer in permafrost areas; the practicability of the
      use of physical-chemical and rotating bio-disc treatment plants; the use
      of  lagoon systems to serve isolated institutions; and the use of plasti-
      cized paperbags  to replace garbage cans.
	.  Hyde,  B.C.   et al.   In press.


 392.  Hynes,  H.B.N., 1969.   The Enrichment of Streams,  pp. 188-196.  In:
         Eutrophication:  Causes,  Consequences,  and Corrections. Printing and
         Publishing Office,  National Academy of Sciences, Washington, D.C.

         The  term eutrophic,  with its connotations of aging and evolution, can-
      not be  properly applied to running water.  Streams change along their
      length,  increasing in  discharge,  width, depth, temperature, and transport
      of  suspended matter and dissolved solids.  Running water tends to be more
      fertile than standing  water, but primary production is patchy due to tur-
      bulence and washout conditions.  Community respiration is greater than
      photosynthesis is  all  but the most highly enriched streams.  Input of sew-
      age effluent enriches  streams by increasing allochthonous organic matter
      near the effluent  source and by increasing the concentrations of nutrient
      salts  farther downstream.  Additional nutrient inputs occur through
      leaching of agricultural soils (Wisconsin Data—12 Ib. nitrate nitrogen
      and 0.6 Ib. phosphate/square mile per day).  Land clearing, channeliza-
      tion and swamp drainage have converted streams into better producers.
      Stream floras tend to  be opportunistic species (Myriophyllum, Potamogeton,
      Cladophora, Oscillatoria) which will invade newly-enriched substrates.
      Production  of large rivers is dominated by planktonic diatoms and roti-
      fers:  turbidity appears to be major limiting factor.  High temperatures,
      low discharges and low turbidities as found in impoundments are favorable
      for the development of  chlorophycean and myxophycean flora and resultant
      increase of primary production.
 393.  Incropera,  F.  P.,  and J.  F.  Thomas.  1977.   A Model for Oxygen and Bio-
        mass Production in a Mass Algal Culture.   Office of Water Research and
        Technology.   Purdue University, Lafayette, IN.  Washington, DC.
        87 pp.

        The primary purpose was to develop a reliable model for predic*ving the
      yield of a  mass culture of unicellular algae in wastewater treatment
      ponds.  Such  algal cultures  are capable of  producing a high protein food
      supplement.   The model developed determines  both oxygen and algal biomass
      production  and is  based upon the use of available insolation data.  It
      accounts for  spectral effects in the photosynthetically active region, as
      well as directional effects  through delineation of the diffuse and
      collimated  components of  the radiation.  The effects of the air-water
      interface are treated, and predictions of the radiation field within the
      water are used with a representative photosynthesis model to predict the
      hourly yield.   Calculations  have been performed for the Indianapolis, IN,
      region, and comparisons with field data for  similar latitudes reveal that
      the model is  well  suited  for predicting the  maximum yield of mass cul-
      tures.  (NT)

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394. Institute of Water Pollution Control.  1977.  Environmental Pollution and
        Agriculture.   Water Pollution Control 76(4):505-510.

        Examples of pollution caused by agricultural activities and of the
     effects of pollution on agriculture are presented; water-quality criteria
     for spray irrigation and for livestock are given in tables.  The need for
     national guidelines on the use of sewage sludge in agriculture, and the
     polluting effects of aquaculture (fish farming and watercress growing)
     are highlighted. (AL)
395. Isirimah, N.O. and D.R. Keeney.  1973.  Contribution of Developed and
        Natural Marshland to Surface and Subsurface Water Quality.  Tech. Rep.
        73-09.  Wise. Water Resources Center, Madison, WI.
396. Jacobs, S. A., and W. B. Kerfoot.  1976.  Cadmium Accrual in Combined
        Wastewater Treatment-Aquaculture System.  Environ. Sci. Technol. 10(7):
        662-667.

        Trace amounts of cadmium were added to isolated links of a sewage/sea-
     water-plankton-mollusc food chain in an experimental tertiary treat-
     ment-aquae ulture system.  Cd accumulation was studied in two types of
     phytoplankton (a green platymonad and a mixture of diatoms), and in two
     species of mollusc (American oyster and hard clam).  Cd concentrations in
     algae increased rapidly until equilibrium was reached while molluscs
     showed a continuous accumulation when exposed to sea water and algae
     mixtures contaminated with Cd.  Investigation of the pathways of accumula-
     tion in the food chain showed that although oysters and clams can absorb
     Cd direct from sea water, the principal source of accumulation is the
     algae.  Human uptake of Cd through eating shellfish, and the rate
     of accumulation by age are discussed.  Recommended levels of Cd in
     municipal effluent and diluent sea water used for aquaculture are cal-
     culated.  (AL)
397. Janota, T., and O. L. Loucks.  1975.  An Analysis of the Value of Wet-
        lands for Holding Inorganic Phosphorus.  Univ. of Wisconsin, Center
        for Biotic Systems. Madison, WI.
398. Jarman, R.  1976.  Aquaculture as an Alternative Wastewater Treatment
        System, pp. 215-224.  In J. Tourbier and R. W. Pierson, Jr.  (eds.),
        Biological Control of Water Pollution.  Univ. of Pennsylvania Press,
        Philadelphia, PA.
399. Jenkins, S. H.   1973.  Advances  in Water Pollution Research.   Proceedings
        of the Sixth  International Conference held  in Jerusalem, June  18-23,
        1972.  Perg.  Press Ltd.  895  pp.

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        This book contains seventy-one of the papers presented at the 6th
     conference organized by the international Association on Water Pollution
     Research, together with the subsequent discussions and replies.  Sections
     are included on thermal pollution and oxygen transfer; trace metals in
     waters; microbiology and virology of wastewater; pollution of ground
     water;  eutrophication and ecosystems; mathematical modelling; sewers and
     stormwater; sewage treatment; industrial effluents; advanced treatment
     and re-use of wastewaters; water management and water quality; and
     estuarine and marine problems.  Author and subject indexes are provided.
     (AL)
400. Jenne, E. A., and S. N. Luoma.  1977.  Forms of Trace Elements in Soils,
        Sediments, and Associated Waters: An Overview of Their Determination
        and Biological Availability, pp. 110-143.  In H. Drucker and R. E.
        Wildung (eds.), Biological Implications of Metals in the Environment.
        Energy Research and Development Administration Symposium Series No. 42,
        Washington, DC.
401. Jetter, W.  1974.  Animal Populations at Two Cypress Domes, pp. 537-588.
        In H. T. Odum, K. Ewel, and J. Ordway (eds.), Cypress Wetlands for
        Water Management, Recycling, and Conservation.  First Annual Report to
        the National Science Foundation.  Center for Wetlands, Univ. of
        Florida, Gainesville.
402. Jetter, W.  1975.  Effects of Treated Sewage on the Structure and Func-
        tion of Cypress Dome Consumer Communities, pp. 588-610.  In; H.T. Odum,
        K. Ewel, and J. Ordway, and M.K. Johnston (eds.), Cypress Wetlands for
        Water Management, Recycling and Conservation.  Second Annual Report.
        Center for Wetlands, Univ. of Florida, Gainesville.
403. Jetter, W., and L.D. Harris.  1976.  The effects of perturbation on
        cypress dome animal communities, pp. 577-653.  In  H.T. Odum, K.C.
        Ewel, J.W. Ordway, and M.K. Johnston (eds.),  Cypress Wetlands for
        Water Management, Recycling, and Conservation.  Third Annual Report
        to the National Science Foundation.  Center for Wetlands, Univ. of
        Florida, Gainesville.

        Wildlife studies were conducted at three cypress domes located 8 km
     northwest of Gainesville, Alachua County, FL, during 1973-1976.  Arthro-
     pods, amphibians, reptiles, birds, and mammals were sampled to determine
     their response to sewage treatment of the cypress domes.  A chronology of
     events affecting the consumer communities; the methodologies employed,
     with types of sampling devices and techniques illustrated; and the results
     of the study are included.  Food web structures were developed for a nat-
     ural dome, a dome that received secondary treated effluent, and a dome
     that received effluent with a heavy organic load to show the major shifts
     in the energy flow pathways.  (EP)

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404. Jewell, W. J. , R. M. Kabrick, J. J. Madras, and J. R. Bouzoun.   1981.
        Reclamation of Wastewater with Plants in Nutrient Films.  Presented at
        the Water Reuse Symposium II.  Washington, DC.  August 23-28,  1981.

        The present directions in wastewater treatment should emphasize maxi-
     mum pollution control and the recovery of nutrients with minimum capital
     and energy investments.  This is the first comprehensive report  on a
     unique plant production system, which could lead to a new solarpowered
     pollution control/resource recovery system.  It could serve as a  major
     tool in achieving high water quality and resource recovery for the
     future.
        The nutrient film technique  (NFT) is a unique modification of  a hydro-
     ponic plant growth system which utilizes plants growing on an impermeable
     surface.  A thin film of water  flowing through the extensive root system
     provides plant nutrients. Exceptionally high annual plant yields, 90  tons/
     ha-yr  (40 tons/acre per year),  and root mass up to 30 cm thick or more
     have been obtained.  It was hypothesized that this self-generating plant
     system could be modified for use as a filter to immobilize and use the
     gross and trace organics in wastewater.  The goal of this study  is to
     determine the economic, technical, and practical feasibility of  using
     plants grown in the NFT system  as pollution control systems.
        This feasibility study has been divided into two phases-a preliminary
     study to establish the potential of the system, and an additional 9-month
     test phase to be completed in June 1981 , to enable additional variables
     to be evaluated in longer term  plant growth tests.  Three parallel
     testing programs were used: pure substrate definition of candidate
     species culturing practices and nutrient responses, synthetic polluted
     wastewater, and primary settled domestic sewage.  These three substrates
     allow measurements of gross pollutant removal, as well as nutrient and
     trace  organic removal kinetics.  The three initial test systems, using  a
     total  of nine test units, were  operated at daily application rates of 2.5
     to 40  cm per day, and flow rates of more than 4000 liters per day.
        The successful cultural experiments have produced, from seed  or
     cuttings, viable stands of Reed Canary Grass, Coastal Bermuda Grass,
     Watercress, Napier Grass, and Cattails in a maximum of six weeks. The
     propagation of NFT Reed Canary  Grass and its use in  solar  greenhouses
     that require little, if any, energy input anywhere in the U.S. has been
     successful.
        At  daily  loading rates of 10 cm per  day the effluent  quality  with
     primary  settled  sewage was often less than 10 mg/1 for SS  and 8005.   The
     influent sewage temperature was 9°C.  Estimated  area needs of an NFT
     system designed  for BOD and SS  removal  appear to be  approximately 2
     hectares per thousand cubic meters  (20  acres per million  gallons) of
     flow,  whereas up to 10 times this  amount may be  needed to  provide ter-
     tiary  quality effluent.  The latter portions of  this study will  include:
     additional plant yield information, valuable by-product  recovery poten-
     tial  (food,  fiber, energy and distilled water),  and  full  scale  system
     design and start-up requirements.   (AA)
 405. Johnson,  R.  R.,  and J.  F.   McCormick (eds.).   1978.  Strategies for
        Protection  and Management of Floodplain Wetlands and Other Riparian
        Ecosystems.   Proceedings of the Symposium, December 11-13, Callaway
        Gardens,  Georgia.  General Tech. Rept. WO-12.  U.S. Forest Service,
        Washington, DC.  410 pp.

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406. Jones, R.C. 1979.  Design and Construction Aspects of the Houghton Irri-
        gation Project.  In; J.C. Sutherland and R.H. Kadlec  (eds.), Wetland
        Utilization for Management of Community Wastewater.  Abstracts of a
        Conference held 10-12 July 1979.

        The unique feature about the Roscommon Township Treatment Facility,
     which is one of several in the Houghton Lake area, is that secondary
     effluent can be disposed of at that site by flood irrigation to seepage
     beds, irrigation fields, or a natural wetland of 600 acres which provides
     tertiary treatment.
        Initial construction of wastewater facilities was begun in  1972 and all
     of the above facilities except for the marshland irrigation project were
     completed by 1974, however, it was anticipated previously that by 1978,
     additional irrigation areas would be required.  Projections made in early
     1970 indicated an additional 98 million gallons of irrigation  capacity per
     year would be needed by 1988, and an additional 181 million gallons by
     1998.
        The most attractive alternative for additional irrigation areas was
     using a nearby State owned swamp for tertiary treatment.  The  cost for
     this method of providing additional irrigation area was much more attrac-
     tive due to the considerable cost savings involved for purchasing of addi-
     tional land and construction of the required facility.
        The wetland was left in its natural state.  It was decided to disperse
     the effluent through gated irrigation pipe.  (AA)
407. Jones, R.A. and G.F. Lee.  1978.  An Approach for the Evaluation of the
        Efficacy of Wetlands-Based Phosphorus Control Programs on Downstream
        Water Quality, pp. 217-243.  In! M.A. Drew (ed.), Environmental Quality
        Through Wetlands Utilization.  Coordinating Council on the Restoration
        of the Kissimmee River Valley and Taylor Creek-Nubbin Slough Basin.
        Tallahassee, FL.  243 pp.

        The Kissimmee River Valley and Taylor Creek-Nubbin Slough Basin Restor-
     ation Project has as one of its objectives a reduction of phosphorus
     transport from agricultural lands to nearby water-courses.  Any project of
     this type with this objective should include an evaluation of the
     potential impact of the nutrient control program on downstream water qual-
     ity.  In the past several years, significant advances have been made in
     the ability to predict with a fair degree of reliability, the impact on a
     water body's water quality, of the control of phosphorus input to the
     waterbody by a certain amount.  These predictions are based on the Vollen-
     weider OECD eutrophication study modeling results where it has been found
     for a wide variety of water bodies located in Western Europe, North Ameri-
     ca, and Japan, that the phosphorus load to a water body normalized by its
     mean depth and hydraulic residence time, can be used to predict the
     average and maximum growing season chlorophyll concentrations, water clar-
     ity based on Secchi depth, and hypolimnetic oxygen depletion rates.
        A discussion will be presented of the use of the OECD eutrophication
     study results to estimate the improvement in water quality which would be
     expected to result from the construction of wetlands in the project area.
     Particular attention will be given to the types of modifications of the
     OECD eutrophication modeling approach that may have to be made in order to
     apply this approach to the water bodies downstream from the project area.
     (AA)

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408. Jones, R.D. and M.A. Hood, 1980.  Effects of Temperature, pH, Salinity,
        and Inorganic Nitrogen on the Rate of Ammonium Oxidation by Nitrifiers
        Isolated from Wetland Environments.  Microbial Ecology 6(4):339-347.

        The transformation of nitrogen and its recycling are extremely import-
     ant to the productivity of aquatic ecosystems.  Although factors affecting
     ammonium oxidation in agricultural soils and sewage have been investigated,
     information on factors affecting nitrification in wetland environments is
     lacking.
        Ammonium-oxidizing bacteria were examined in a freshwater marsh and in
     an estuarine bay over a 2-year period.  Two predominant species of Nitro-
     somonas were consistently isolated, one from each environment.  A closed
     culture, high cell density assay was used to determine the effects of
     temperature, pH, salinity, sodium, potassium, nitrite, nitrate, and ammon-
     ium concentrations on nitrification.  Maximum oxidation of ammonium by the
     freshwater isolate occurred at 35 degrees C, pH 8.5, salinities of 0.3 to
     0.5 percent sodium and potassium, and ammonium concentrations exceeding
     0.5 grams per liter.  The estuarine isolate exhibited maximum activity at
     40 degrees C, pH 8.0, salinities of 0.5 to 1.0 percent sodium and potass-
     ium, and 0.2 grams of ammonium per liter.  The sodium requirement of the
     estuarine isolate could be partially substituted by potassium, suggesting
     that the organism is a true estuarine bacterium.  Salinities below 0.5
     percent may severely reduce oxidation of ammonium by the bacterium in the
     estuarine environment.  While nitrate had no significant effect on either
     isolate, certain combinations of ammonium nitrite were inhibitory, espe-
     cially to the estuarine species.  The data indicate that oxidation of
     ammonium by isolates from wetlands is influenced by pH and temperature and
     that estuarine nitrifiers may be more sensitive to and more extensively
     affected by salinity, ammonium concentrations, and nitrite than are
     isolates from fresh waters.
409. Jorek, N. 1977.  Habitat Management Concepts Worked Out at the Sewage Farm
        Project at Muenster.  Agnew Ornithology 5(2):57-108.
410. Joseph, J. 1978.  Hyacinths for Wastewater Treatment.  Reeves Journal.
        56(2):34-36.

        Secondary treatment of wastewater with water hyacinths produced signif-
     icant reductions in impurities, coliforms, and heavy metals in pilot plant
     tests conducted by the National Space Technology Laboratory at Orange
     Grove, Mississippi.  Biologically treated wastewater from a population of
     5,000 was introduced into an 0.7-acre hyacinth lagoon which was four to
     five times smaller than the primary aerated lagoon.  Retention times of
     14-21 days in the lagoon reduced suspended solids by an average of 72%,
     total nitrogen by 60%, total phosphorus by 26%, BOD to below standard
     levels, and coliforms from 121,000 to 40,000/100 ml. In the absence of
     toxins or metals, the harvested hyacinths yielded up to 25% crude protein
     for supplemental animal feed.  An acre of hyacinths, producing from 8-16
     tons wet bio-mass/day, generated 3,200-6,450 cu ft of methane gas/day with
     0.5 ton fertilizer as a by-product.  The water hyacinths were effective in
     removing lead, mercury, cadmium, cobalt, nickel, silver, and phenols.  Re-

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     search is being conducted to expand the hyacinth belt north of 33 degrees
     latitude.
411. Jurdi, M. S., A. Acrea, and M. Soufi.  1981.  Wastewater Reclamation by
        Hydroponic Plant Growth.  Presented at the Water Reuse Symposium II.
        Washington, DC.  August 23-28, 1981.

        The feasibility of growing plants hydroponically in raw untreated
     wastewater for reclamation and reuse was investigated.  A variety of
     plants (cucumbers, tomatoes, peppers, and lettuce) were successfully
     grown in the medium but required the addition of iron.
        The yield of vegetative growth in the medium was about double that in
     half-strength Hoaglands nutrient solution.  Monitoring macro-elements
     (e.g., nitrate, sodium, potassium, phosphate, calcium), and trace ele-
     ments (e.g., zinc, copper, manganese) showed a substantial reduction in
     their concentrations in the medium.  Reduction of the mineral content
     correlated exponentially with time to a specific conductivity level of
     (300/umohos/cm, 25°C) equivalent to that of tap water.  Transplanting
     into the medium of seedlings at more advanced stages of growth substan-
     tially lessened the period required for reduction in the mineral content
     of wastewater.  This reduction correlated significantly with the final
     dry weight of the plants at the 0.05 level (r=0.979).  (This work is
     supported by grants from The National Council for Scientific Research,
     Beirut, Lebanon).  (AA)
412. Jurriens, M., and G. J. Kaassen.  1980.  I. Evaluation of the Possibili-
        ties and the Effects of Bypassing Water Along Marshy Areas, and II*
        Sediment Intake to Reduce Degradation in an Offtake Canal.  Rept. No.
        226.  Delft Hydraulics Laboratory.  The Netherlands.
413. Kadlec, J.A. 1962.  Effects of a Drawdown on a Waterfowl Impoundment.
        Ecology 43:167281.
414. Kadlec, J. A.  1976.  Dissolved Nutrients in a Peatland Near Houghton
        Lake, Michigan, pp. 25-50.  In D. L. Tilton, R. H. Kadlec, and C. J.
        Richardson (eds.), Proceedings of a Symposium on Freshwater Wetlands
        and Sewage Effluent Disposal.  Held 10-11 May 1976 at the Univ. of
        Michigan, Ann Arbor.

        The use of peatlands and other wetlands as tertiary sewage treatment
     systems depends in part on a knowledge of the nutrient dynamics.  The
     studies of dissolved nutrients reported in this paper were designed to
     contribute to that knowledge.  The objectives were threefold:

       To establish the levels and variability of the principle dissolved
       nutrients as background for other studies;

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        To learn something of the causal mechanisms involved;

        To estimate the impact of controlled fertilization on the concentrations
        of dissolved nutrients.
 415.  Kadlec,  J.  A.   1979.   Nitrogen and Phosphorus Dynamics in Inland Fresh-
         water Wetlands,  pp. 17-41.   Iii T.  A.  Bookhout (ed. ),  Waterfowl and
         Wetlands -  An Integrated Review.  Proceedings of a Symposium held at
         the 39th Midwest Fish and Wildlife Conference at Madison, WI, 5
         December 1977.   La Crosse Printing Company, La Crosse, WI.


1080.  Kadlec,  J.A.   In press.
 416.  Kadlec,  J.  A.,  R.  H.  Kadlec,  P.  E.  Parker, and K. R. Dixon.  1973.  The
         Effects  of Sewage  Effluent on Wetland Ecosystems.  Progress Rept. to
         the National Science Foundation  for 1 July 1972 to 1 April 1973.
         Univ.  of Michigan,  Ann Arbor.
 417.  Kadlec,  J.  A.,  R.  H.  Kadlec,  and C.  J.  Richardson.  1974.  The Effects of
         Sewage Effluent on Wetland Ecosystems.   Semi-Annual Rept.  No. 1 to the
         National Science Foundation.   Univ.  of  Michigan, Ann Arbor.
 418.  Kadlec,  J.  A.,  R.  H.  Kadlec,  and C.  J.  Richardson.  1974.  The Effects of
         Sewage Effluent on Wetland Ecosystems.  Progress Rept. to the National
         Science Foundation 1  April 1973 to 1 March 1974.  Univ. of Michigan,
         Ann Arbor.
 419.  Kadlec,  R.  H.   1975.   Pilot Scale Irrigation Experiment - 1975; Prelim-
         inary and Partial  Results.   In R.  H.  Kadlec,  C. J.  Richardson, and J.
         A.  Kadlec (eds.),  The Effects of Sewage Effluent on Wetland Ecosys-
         tems.  Semi-annual Rept.  No. 4.   Univ.  of Michigan, Ann Arbor.


 420.  Kadlec,  R.  H.   1976.   Surface Hydrology of Peatlands,  pp. 5-24.   In
         D.  L. Tilton,  R. H.  Kadlec,  and C. J.  Richardson (eds.),  Proceedings
         of  a Symposium on  Freshwater Wetlands and Sewage Effluent Disposal.
         Held 10-11  May 1976 at the Univ. of Michigan,  Ann Arbor.

         Annual precipitation exceeds evaporation and drainage is relatively
      poor in peatlands.  Throughflow is  slow enough to permit peat deposition
      and permit growth of  moisture-tolerant plants.  Water depth, nutrient
      availability and  climatic conditions combine with successional processes
      to determine the  type of vegetation in a given wetland situation.
         Any process which  affects the water content of such an area will, in
      the long term, affect the character of the wetland.  Large-scale addition
      of wastewater  will  tend to increase water depths, and at some scale, will

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     produce an environment conducive  to  deep-water  species  such as cattail.
     It is necessary to understand and quantify  the  water  budget of a given
     wetland in order that wastewater  addition can be  intelligently planned.
        In July, August and September  1975, wastewater was added to a small
     site in the peatland.  The pilot  (10-acre)  site is  in a shallow depres-
     sion, with a mean ground of  10.5  feet with  respect  to the  datum point of
     10.00 feet located at station Z-4.   A "ridge" of  elevation 10.75 feet
     along the 68-line effectively "dams" a 200-acre area  enclosing the pilot
     area.
        Wastewater addition amounted to 8 inches on  10 acres in 8 weeks.  No
     level effects were observed  in the test area during irrigation.   Levels
     increased and decreased in accord with the  rest of  the  peatland.  There  is
     a possible small increase in the  water depth for  the  entire 200-acre
     locale, but the topography negates the possibility  of cross-country dis-
     persal.  No increases in peatland outflow were  observed.   Based on the
     data, it appears that a major portion of the added  water evaporated from
     the test locale.
        Larger-scale additions of water will increase  depths and throughputs.
     However, the flow of water added  at  up-gradient points  will be uniform,
     and at the same linear velocity as that in  the  natural  peatland. Thus, the
     added water will be in contact with  the surface litter  for a period of
     approximately 100 days.  The amount  of added evaporation,  if any,  will
     depend upon changes in the plant  cover type and density, and in water
     level.  (AA)
421. Kadlec, R. H., 1977a. Natural and Irrigation Hydrology  of  the Porter Ranch
        Peatland, pop. 198-229.  In; C.B. DeWitt and E.  Soloway (eds.),  Wet-
        lands Ecology,, Values, and Impacts.  Proceedings  of the Waubesa Con-
        ference on Wetlands held in Madison, WI, 2-5 June  1977.  Institute for
        Environmental Studies, Univ. of Wisconsin, Madison.
422. Kadlec, R.H. 1977b. Pilot Scale Irrigation Hydrology, pp.  30-52.   In;
        R.H. Kadlec, D.L. Tilton, and J.A. Kadlec  (eds.).  Feasibility of
        Utilization of Wetland Ecosystems for Nutrient  Removal  from Secondary
        Municipal Wastewater Treatment Plant Effluent.   Semi-Annual Rept. No.
        5.  Univ. of Michigan, Ann Arbor.

        Over 10 million gallons of secondarily treated  wastewater were pumped
     to a northern Michigan peatland and distributed via a 200  meter gated
     irrigation pipe during the period May-September 1976.  The added water
     spread in an irregular concentric pattern, creating a very shallow mound
     of surface water.  The area of the mound ranged upward from 40,000 m^  at
     an average depth of about 3 cm, depending upon precipitation and evapo-
     transpiration.  The prevailing drought conditions  created  a dry soil
     condition surrounding the pumped water mound.  A concentric elliptical
     ring compartment budget was developed to summarize details of water
     movement and storage.  (AA)
423. Kadlec, R.H. 1979a. Monitoring Report on the Bellaire Wastewater Treatment
        Facility.  Utilization Rept. No. 3.  Univ. of Michigan, Ann Arbor.

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424. Kadlec, R. H.  1979b. Monitoring Report on the Bellaire Wastewater Treat-
        ment Facility.  Utilization Rept. No. 4.  Univ. of Michigan, Ann
        Arbor. 48 pp.

        Wastewater from stabilization lagoons is gravity-discharged  during
     stammer and autumn to a partially forested peatland at Bellaire, MI.  The
     results of studies conducted over a five year period show good  phosphorus
     and nitrogen removal.  Some damage has occurred to trees.
425. Kadlec, R.H. (ed.).  1979c. Wetland Utilization for Management  of Com-
        munity Wastewater - 1978 Operations Summary.  Report to the  National
        Science Foundation.  Univ. of Michigan, Ann Arbor.  107 pp.
426. Kadlec, R. H.  1979d. Wetlands for Tertiary Treatment.  In P. K. Greeson,
        J. R. Clark, and J. E. Clark (eds.), Wetland Functions and Values: The
        State of Our Understanding.  Proceedings of the National Symposium on
        Wetlands, Lake Buena Vista, FL.  Am. Water Resour. Assoc., Tech. Publ.
        TPS79-2.  Minneapolis, MN.

        Current information on the performance of fresh water wetlands  in
     tertiary wastewater treatment is reviewed.  A significant potential for
     water quality improvement has been demonstrated at many sites.  Different
     choices of wetland partitioning are examined and illustrated, including
     the entire ecosystem, transects, and plots.  Various  derived measures of
     performance are illustrated, and all have some merit.  Time effects are
     explored on several scales.  Diurnal, daily, annual,  and geological
     variations are all important.  Data are lacking in many categories, but
     further studies are clearly warranted.  Digital computation is  necessary
     to organize the available data and extrapolate to different times, sites,
     and management strategies.  Design criteria are not yet available, but
     economics appear favorable.  (AA)
427. Kadlec, R. H.  1979e. Performance of the Bellaire, Michigan,  Wetland
        Treatment Facility.  In J. C. Sutherland and R. H. Kadlec  (eds.),
        Wetland Utilization for Management  of Community Wastewater.   Abstracts
        of a conference held 10-12 July at  Higgins Lake, MI.


        The village of Bellaire (pop. 1,000) is located in the  northwestern
     portion of Michigan's lower peninsula, on the Intermediate River,  which
     flows into Lake Bellaire.  To protect  the lake from unnecessary nutrient
     loading, secondary and tertiary stage  treatment of wastewater is achieved
     at a considerable saving to the village, by surface discharge onto a con-
     iferous forest wetland.  The design and method of discharge at Bellaire
     was intended to be as similar to Houghton Lake as possible.  Despite a
     vegetational dissimilarity, nutrient removals from wastewater at Bellaire
     have been as efficient as at Houghton  Lake.
        Wastewater was pumped to the wetland during the summers of 1976 and
     part of 1977, and a  gravity system was used thereafter.  Data were

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     acquired on water  flows, water  chemistry,  and environmental factors during
     all three summers.  Summer averages  of  total  dissolved P ranged from 2.11
     to 3.48 mg/1; while the wetland outfall ranged from 0.06 to 0.16 mg/1.
     This is a concentration reduction  of 94-97%.  Summer averages of dissolved
     N ranged from 1.94 to  10.84 mg/1;  while the wetland outfall ranged from
     0.04 to 0.61 mg/1.  This is a concentration reduction  of 91-98%.   About
     one-third of these reductions are  due to dilution.   Water samples taken
     during late winter and early spring  1977 indicated  a release of P equal to
     1.02% of the total P added over the  period March-November.   (AA)
428. Kadlec, R. H. 1979f.  Performance  of the Houghton Lake, Michigan,  Wetland
        Treatment Facility.  In J. C. Sutherland  and R.  H. Kadlec  (eds.),
        Wetland Utilization for Management of Community  Wastewater.   Abstracts
        of a conference held 10-12 July at Higgins Lake, MI.

        The Houghton Lake Wetland Treatment System was built and placed in
     operation during the first six months of 1978.  An  operation  and mainte-
     nance plan has been developed, and the system operated successfully in  the
     treatment of 65 million gallons of secondary wastewater in summer  1978.
        Water depths in the irrigation  area were  approximately 10-20  cm, with
     patches of deeper water in isolated depressions.  Nitrogen and phosphorus
     removal was typically 90% complete within  100 meters of the discharge.
     Chloride was mostly unaffected by  passage  through the wetland.   Altered
     water chemistry persisted further  downgradient in the deeper  water areas.
     The water in the discharge area has recovered completely by mid-October.
     No dramatic changes in vegetation  species  composition occurred in  this
     first year, but plant growth and chlorophyll content were increased with-
     in 30 m of the discharge.  No large changes  occurred in vertebrate use,
     but there were alterations in vertebrate species composition  and abundance
     within 30 m of the discharge.
        The Houghton Lake wetland is well suited  for use as a tertiary  treat-
     ment facility due to several natural features and functions:  deep  organic
     soils, low nutrient levels in the  background water, high plant productiv-
     ity, and slow water movement.  (AA)
429. Kadlec, R. H. 1979g.  Wetland Tertiary Treatment at Houghton Lake,
       . Michigan,  pp. 101-139.  In R. K. Bastian and S. C. Reed (eds.),
        Aquaculture Systems for Wastewater Treatment: Seminar Proceedings and
        Engineering Assessment.  U.S. Environmental Protection Agency, Office
        of Water Program Operations, Municipal Construction Division.  Wash-
        ington, DC. EPA 430/9-80-006.

        The effects of secondary treated wastewater applied to a peatland in
     central Michigan for five consecutive summers are documented in this
     report.  The Houghton Lake wetland treatment system was built and placed
     in operation during the first six months of 1978 and has proved to be an
     effective means of nutrient removal.  Numerous figures and tables are
     included in the report that explain the effects of the study on wetland
     hydrology, water quality, vegetation, soils, algae, vertebrate and inver-
     tebrate populations, and pathogenic organisms. (EP)

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430. Kadlec, R. H.  1981a. Monitoring Report on the Bellaire Wastewater Treat-
        ment Facility.  Utilization Rept. No. 5.  Univ.  of Michigan, Ann
        Arbor.
431. Kadlec, R. H. 1981b.  Monitoring Report on the Bellaire Wastewater Treat-
          ment Facility.  1980.  Wetlands Ecosystem Research Group,  Univ.  of
          Michigan, Ann Arbor. 56 pp.

          Approximately 30 million gallons of effluent were discharged to  a
     wetland treatment site at Bellaire, Michigan, during the period 4 June to
     5 November 1980.  Data on water flows, water chemistry and  other environ-
     mental parameters were collected and analyzed.  Concentration  reductions
     of 90% total dissolved phosphorus and 91% dissolved nitrogen were
     achieved in passage of the water through the wetland.
        A five year summary of data for the performance of the wetland is
     included.  Gross removal of dissolved nitrogen remained stable at ap-
     proximately 78%.  Gross removal of phosphorus remained at 65%.  As a
     consequence, the amount of phosphorus discharged to Lake Bellaire has
     remained at five times the background level in the natural  wetland.   (EP)
432. Kadlec, R.H.  1981c.  How Natural Wetlands Treat Wastewater, pp.  241-254.
        In; B. Richardson (ed.), Selected Proceedings of  the Midwest Conference
        on Wetland Values and Management.  Minnesota Water Planning Board.   St.
        Paul.  660 pp.

        The advanced treatment of wastewater consists of  removing the  last
     traces of dissolved and suspended materials, pathogens and perhaps  other
     biota.  A wetland provides a blend of the aquatic and terrestrial eco-
     system functions which have been proven to remove these undesirable mater-
     ials.  The incoming waters, typically slow to creeping flow, provide set-
     tling time for suspended material.  Physical filtration through litter
     material also can occur.  The wetland produces detritus and algal debris,
     which partly contribute to the pool of suspended and suspendable  solids.
        Dissolved nutrients can be sorbed on soil surfaces in  shallow  waters if
     given sufficient time to reach that surface.  Bacterial action, such as
     denitrification, can occur at these surfaces as well.  Typically, phos-
     phorus compounds sorb and precipitate but nitrogen compounds do not.
     Nutrients are also used by algae and vascular plants in formation of new
     tissues on a seasonal basis.  Subsequent death and litter fall of all or
     some algae and plant parts (leaves) occurs.  The litter then decomposes,
     releasing the stored nutrients.  However, a small portion becomes a
     permanent component of the wetland soil.  Algal decomposition  is  rapid
     (weeks), and the detritus is finely divided.  Plant  litter decomposition
     is slow (years), and the detritus is coarse.
        Addition of nutrients builds a larger vegetative  pool, and  hence a
     larger litter pool.  Significant amounts of material are  required.
        Other dissolved materials may be stored in the vegetative/litter pool,
     or sorbed, as well.  Elements such as boron and sulfur display this behav-
     ior.  Other elements, such as sodium and chloride, are passed  through with
     little effect-.

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         Bacteria of many kinds thrive in the wetland environment, including
      coliforms.   This serves to confuse many health-related issues.  Viruses
      appear to deactivate at variable rates, ranging from hours to weeks.  More
      research is needed in these areas.  (AA)
1081.  Kadlec, R.H.  1983.

1082.  Kadlec, R.H.  In press.


 433.  Kadlec, R. H., and D. E. Hammer.  1980.  Wetland Utilization for Manage-
         ment of Community Wastewater - 1979 Operations Summary, Houghton Lake
         Wetlands Treatment Project.  Report to the National Science Foun-
         dation.  Univ. of Michigan, Ann Arbor. 83 pp.

         The Houghton Lake Wetland Treatment System operated successfully in
      the treatment of 65 million gallons of secondary wastewater in the summer
      of 1978, and 101 million gallons in 1979.  Treated wastewater from a
      holding pond is pumped through a 12 inch diameter underground force line
      to the edge of the Porter Ranch peatland. The water is distributed even-
      ly across the width of the peatland through small gated openings in the
      discharge pipe.  Each of the 199 gates discharged approximately 16 gal-
      lons per minute, under typical conditions, and the water spreads slowly
      over the peatland.  Results are presented under the following topic areas:
      system operation, hydrology, surface water chemistry, litter and soil,
      vegetation status and response, and algal productivity.  (NT)


 434.  Kadlec, R. H., and D. E. Hammer.  1981.  Wetland Utilization for Manage-
         ment of Community Wastewater - 1980 Operations Summary, Houghton Lake
         Wetlands Treatment Project.  Report to the National Science Foun-
         dation.  Univ. of Michigan, Ann Arbor. 76 pp.
 435. Kadlec, R. H., D. E. Hammer, I. S. Nam, and J. O. Wilkes.  Overland Flow
         in Wetlands.  Presented at the 88th National Meeting, American Insti-
         tute of Chemical Engineers, 1980.


 436. Kadlec, R. H., D. E. Hammer, and D. L. Tilton.  1978.  Wetland Utiliza-
         tion for Management of Community Wastewater.  Status Rept. to the
         National Science Foundation.  Univ. of Michigan, Ann Arbor. 59 pp.
         (Available as NTIS Report PB80-108228).

         Studies resulting in high water quality of the Houghton Lake Wetland
      Treatment system are reported.  This system successfully treated 65
      million gallons of secondary wastewater in the summer of 1978 for use  in
      irrigating the Porter Ranch wetland in Michigan.  Field research at the
      wetland focused on: (1) Collection of data to establish a current picture
      of the ecosystem in the discharge area; (2) continuous monitoring of the
      response of the irrigation area to wastewater flow; and  (3) continuation
      of fundamental field studies to better understand response mechanisms  and
      wetland system limits.  Specific aspects described include water chem-
      istry, soil elevation sampling, vegetation analysis, algae studies,

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      nutrient sorption, bacteria and virus assays, and census of vertebrates
      and invertebrates.  Laboratory studies were conducted to measure the
      rates and extent of physiochemical sorption of ammonia, nitrate, and
      phosphate.   (NT)
1083. Kadlec, R.H. and D.E. Hammer.  1982.

1084. Kadlec, R.H. and D.E. Hammer.  1983.
 437.  Kadlec, R. H., D. E. Hammer, D. L. Tilton, L. Rosman, and B. Yardley.
         1978.  Boughton Lake Wetland Treatment Project.  First Annual Oper-
         ations Report.  Univ. of Michigan, Ann Arbor. 87 pp.
 438.  Kadlec,  R.  H.,  and J. A. Kadlec.  1979.  Wetlands and Water Quality
         (Theme Paper), pp. 436-456.  In P. E. Greeson, J. R. Clark, and J. E.
         Clark (eds.),  Wetland Functions and Values: The State of Our Under-
         standing.  Proceedings of the National Symposium on Wetlands, Lake
         Buena Vista, FL, Am. Water Resour. Assoc., Tech. Publ. TPS 79-2.
         Minneapolis, MN.

         Wetland waters interact strongly with the other biotic and abiotic
      components of the ecosystem.  Every water quality parameter is altered by
      passage through a wetland ecosystem.  Nutrients and other dissolved
      constituents, heavy metals,  suspended solids, and bacteria move into and
      out of the wetland with entering and leaving waters.  Their concentra-
      tions can be altered by uptake, cycling, and dilution.  Studies have been
      made of the gross inputs, outputs, and storages within wetlands; as well
      as more detailed studies of the water, sediment, and floral compartments.
      To interpret the performance of a wetland in altering any water quality
      parameter,  a knowledge of the hydrology of the wetland is required, along
      with data on concentrations, mass flow and storage of the constituent of
      interest.  Wetland ecosystems are dynamic, with both stochastic and time
      varying effects being of great importance.  Diurnal, seasonal, and
      historic time patterns are necessary for complete understanding.  Nitro-
      gen and phosphorus cycle from the water to the plants to the sediments in
      the wetland ecosystem, with net storage or release depending upon the
      site.  Dissolved constituents, such as chloride, often pass through the
      system without much alteration.  Heavy metals appear to be immobilized in
      many situations,  as are pesticides and other refractory chemicals.
      Suspended solids  play an important role in wetland functions, both by
      themselves and as transporters of other components.  Although wetlands
      are microbially active, they appear to be intolerant of human enteric
      microorganisms.  These ecosystems appear to function as dynamic buffers
      against external  upsets.  In many cases, they are capable of serving as
      disposal sites for treated wastewaters.  In almost all areas, there is a
      need to expand the data base in order to gain a better understanding of
      wetland processes which affect water quality.  (AA)
 439.  Kadlec, R.H. and J.A. Kadlec, 1974.  The Effects of Sewage Effluent  on
         Wetland Ecosystems.  NSF-RANN Semi-Annual Report.  Univ.  of Mich.  Ann
         Arbor, MI.

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440. Kadlec, R.  H,, J.  A.  Kadlec,  C.  J.  Richardson,  and D. L. Tilton.  1975.
        The Effects of  Sewage Effluent on Wetland Ecosystems.  Semi-Annual
        Rept. No.  4.  Univ.  of Michigan,  Ann Arbor.  197 pp.

        The report is part of an  ongoing study of the nutrient status of the
     surface and interstitial waters  of peatlands in Michigan.  The contact of
     influent water with mineral  soil and the length of travel through peat
     after such  contact are  key factors in determining retention.  The balance
     between influent ground water and rain water as sources of nutrients for
     plant growth are considered  to determine the plant communities.  This
     study has been directed at an examination of the major ecosystem com-
     ponents with a view to  predicting and monitoring these changes.  This
     report considers the  dissolved nutrients of  the peatland, their variation
     in time and space, and  the impact of small scale nutrient additions. (NT)
441. Kadlec, R. H., C. J. Richardson,  and J.  A.  Kadlec.   1974.   The Effects of
        Sewage Effluent  on Wetland Ecosystems.   Semi-Annual Rept. No. 2 to the
        National Science Foundation.   Univ.  of Michigan,  Ann Arbor.
442. Kadlec, R. H., C. J. Richardson,  and J.  A.  Kadlec.   1975a.   The Effects of
        Sewage Effluent on Wetland Ecosystems.   Semi-Annual Rept. No. 3 to the
        National Science Foundation.   Univ.  of Michigan,  Ann Arbor.
443. Kadlec, R. H., C. J. Richardson,  and J. A.  Kadlec.   1975b.   The Effects of
        Sewage Effluent on Wetland Ecosystems.   Semi-Annual Rept. No. 4 to the
        National Science Foundation.   Univ.  of Michigan,  Ann Arbor.

        Ongoing studies of the  effects of sewage effluents  in wetland eco-
     systems are reported, with particular emphasis  on the  nutrient  status of
     the surface and interstitial waters  of  peatlands in  Michigan.  Specific
     topics covered include: the effects  of  simulated sewage effluent on the
     decomposition, nutrient status, and  litterfall  in a  central Michigan peat-
     land, the effects of simulated sewage effluents on the growth and produc-
     tivity of peatland plants, pilot  scale  irrigation experiments,  a statisti-
     cal analysis of seasonal changes  in  biomass (standing  crop  new  growth)  of
     selected wetland species as influenced  by nutrient additions, and studies
     of dissolved nutrients in Michigan peatland.
444. Kadlec, R. H., and D. L. Tilton.   1977.  Monitoring Report  on the
        Bellaire Wastewater Treatment Facility.  Utilization  Rept.  No.  1.
        Univ. of Michigan, Ann Arbor.   (Two versions: complete and abridged).
445. Kadlec, R. H., and D. L. Tilton. 1978a.  Monitoring Report  on  the  Bell-
        aire Wastewater Treatment Facility.  Utilization Rept. No.  2.   Univ.
        of Michigan, Ann Arbor.

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        This report concerns a secondary and tertiary stage treatment of
     wastewater by the village of Bellaire, MI, by surface discharge of ef-
     fluent onto a forested wetland.  Water flows, water chemistry, and en-
     vironmental data were collected for an 8 month period.  Total dissolved
     phosphorus and nitrogen in the wastewater averaged 3.48 mg per liter and
     4.95 mg N/liter during the discharge period.  Concentrations of P and N
     in water leaving the wetland were 0.11 mg/1 and 0.46 mg/1 respectively.
     This represents concentration reductions of 97% TNP and 91% DN.  (WE)
446. Kadlec, R.H., and D.L. Tilton.  1978b. Wastewater Treatment Via Wetland
        Irrigation: Nutrient Dynamics,  pp. 153-170.  In M. A. Drew  (ed.),
        Environmental Quality through Wetlands Utilization.  Proceedings of a
        Symposium held 28 February - 2 March 1978, at Tallahassee, FL.
        Sponsored by the Coordinating Council on the Restoration of the
        Kissimme River Valley and Taylor Creek-Nubbin Slough Basin.  Talla-
        hassee, FL.

        The impact of discharging approximately 10 million gallons  of  sec-
     ondary effluent into a 10 acre section of the Houghton Lake wetland is
     described.  Element budgets showed that 99%, 95%, and 71% of the  total
     input of nitrate and nitrite nitrogen, total dissolved phosphorus (TDP),
     and ammonia nitrogen, respectively, were immobilized within 30 meters of
     the discharge location.  Element immobilization occurred by a  combination
     of biological and physical processes.
        The Bellaire wetland was monitored from 15 March through 21 November
     1977 to determine water flows, water chemistry, and environmental data.
     TDP and TDN in the wastewater averaged 3.48 mg/1 and 4.95 mg/1 during the
     discharged period (27 May through 3 November).  Concentrations in the
     water leaving the wetland were 0.11 mg-P/1 and 0.46 mg-N/1.  These re-
     present concentration reductions of 97% TDP and 91% TDN.  Water samples
     taken during late winter and early spring indicate a release of phos-
     phorus equal to 1.02% of the total phosphorus added during the period
     March to November.  (EP)
447. Kadlec, R. H., and D. L. Tilton.  1979.  The Use of Freshwater Wetlands
        as a Tertiary Wastewater Treatment Alternative.  CRC Critical Reviews
        in Environmental Control 9(2):185-212.

        The use of wetlands for tertiary wastewater treatment is a feasible
     alternative in the proper circumstances.  The constraints which apply have
     not been totally determined, but are likely to be very site-specific.
     Small communities with large adjacent wetlands might consider this alter-
     native.
        Wetlands appear to be strongly buffered against the stress of treated
     wastewater additions.  Dissolved nutrients are effectively removed in many
     cases, and suspended solids are exchanged.  The added water and nutrients
     have an impact on the ecosystem which varies from site to site.  The plant
     and animal communities undergo changes which range from barely undetect-
     able to dramatic.

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448. Kadlec, R. H., D. L. Tilton, and J. A. Kadlec.   1977.  Feasibility of
        Utilization of Wetland Ecosystems for Nutrient Removal from Secondary
        Municipal Wastewater Treatment Plant Effluent.  Semi-Annual Rept. No.
        5 to the National Science Foundation.  Univ.  of Michigan, Ann Arbor.

        This study details the effect of discharging  approximately 10 million
     gallons of secondary effluent onto a 10 acre section of wetland.  Total
     dissolved phosphorus (TOP), ammonia nitrogen, nitrate plus nitrite nitro-
     gen, Cl, Na, Ca, Mg, K, Ni, Zn, Cu, Pb, COD, pH, alkalinity, hardness,
     and specific conductivity were measured in the effluent and in surface
     waters at various distances (30-225 m) from the  discharge location.  TOP,
     nitrate plus nitrite nitrogen, and pH in surface waters had returned to
     background concentrations within 30 m of the discharge.  Alkalinity and
     hardness had decreased to background concentrations 50 m from the dis-
     charge point, while Cl, Ca, Mg, Na, and to a lesser extent, K, tended not
     to decrease with the distance as much as the more biologically active
     ions.  COD was lower in the effluent than in the wetland's surface water.
     Element budgets showed that 99%, 95%, 71% of the total input of nitrate
     plus nitrite nitrogen, TOP, and ammonia nitrogen, respectively, were
     immobilized within 30 m of the discharge site.   Equivalent immobiliza-
     tions of the total input for K, Mg, Na, Ca, and  Cl, were 69%, 42%, 39%,
     32%, and 30% respectively.  Element immobilization occurred through a
     combination of physical and biological factors.  (WE)
449. Kadlec, R. H., D. L. Tilton, and B. R. Schwegler.  1979.  Wetlands for
        Tertiary Treatment: A Three-Year Summary of Pilot Scale Operations at
        Houghton Lake.  Report to the National Science Foundation.  Univ. of
        Michigan, Ann Arbor, 101 pp.  (NTIS No. PB 295 965)

        The process of wetland tertiary treatment of sewage treatment plant
     effluent was studied.  The site was a peatland located at Houghton Lake,
     MI. The goal was to discharge 100,000 gallons/day treated wastewater onto
     a test plot measuring 40,000 square meters.  This area was a scale up of
     1000 over the earlier test plots.  Monitoring and research activity
     focused primarily on hydrology and water quality, with secondary emphasis
     on plant, soils, animals, algae, bacteria, and viruses.  Criteria for
     stopping effluent discharge were developed.  This report covers the field
     results related to the discharge of wastewater.  The results indicate
     that this method is an effective means of nutrient removal.  All nitrogen
     and phosphorus were stored or removed within a five acre area, at a
     discharge rate of 100,000 gallons per day.  The maximum increase in water
     depth was 15 cm at the center of a single point discharge.  Coliform
     bacteria were present in both the discharge and in the natural wetland in
     comparable numbers.  No virus was transported to the wetland.  The animal
     populations exhibited little response to the discharge.  (NT)
450. Kaila, A.  1959.  Retention of Phosphate by Peat Samples.  J. Sci. Agri.
        Soc. Finland 31:215-225.

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451. Kaminski, R. M., and H. H. Prince.  1981.  Dabbling Duck and Aquatic
        Macroinvertebrate Responses to Manipulated Wetland Habitat.  J. Wildl.
        Manage. 45(1):1-15.

        Responses of breeding dabbling ducks (Anatini) and aquatic raacroinverte-
     brates to experimental modifications of cover; water ratio and basin sur-
     face were investigated in 1977 and 1978 within an impounded whitetop river-
     grass (Scplochloa festucacea) meadow on the Delta Marsh, south-central Man-
     itoba.  Three areal percentage ratios of emergent hydrophytes to open water
     (30:70, 50:50, or 70:30) and 2 basin treatments (mowing of existing emer-
     gents or scarification by rototilling) were tested.  Between years, pair
     numbers of mallards (Anas platyrhynchqs) and blue-winged teal (A. dijcors)
     declined, whereas pair numbers of northern shovelers (A. clypeata),
     gadwalls (A. strepera), and pintails (A. acuta) were comparable.  The
     greatest density and species diversity of dabbling duck pairs occurred on
     50:50 plot in both years.  Only blue-winged teal and pintail pair densities
     in 1978 were greater on mowed than on rototilled areas.  Within years,
     species diversity of dabbling ducks was unaffected by mowing or rototill-
     ing.  More pursuit flights arose from 50:50 plots and mowed areas compared
     to alternative treatments.  Composition and resource levels (abundance,
     biomass, and number of families) of aquatic macroinvertebrate communities
     varied within and between years in response to basin treatments.  These
     results imply prescriptions for wetland habitat management.  (AA)
452. Kamppi, Armas.  1971.  Studies of Wastewater Disposal on Peatland Forest
        Basin Infiltration Systems Which were in Effect in Finland in 1970.
        National Board of Waters, Finland:  Report A4.  120 pp.
453.  Kappel, W.  1979a.  The Drummond Project.  In; J.C. Sutherland and R.H.
        Kadlec (eds.), Wetland Utilization for Management of Community Waste-
        water.  Abstracts of a conference held 10-12 July 1979 at Higgins Lake,
        MI.

        The Town of Drummond (pop. 270), in northwestern Wisconsin, recently
     built a sewage treatment facility to handle the community's septic wastes.
     Plans were initially developed for a three lagoon, secondary treatment sys-
     tem, with effluent discharge twice a year to the Long Lake Branch of the
     White River, a Class 1-A trout stream.  The lagoon system was to be built
     on U.S. Forest Service land, and in reviewing the project's environmental
     assessment, the Forest Service suggested a tertiary treatment alternative
     in which a 27-acre peat bog would be used to remove inorganic nutrients
     before final discharge to this important trout stream.
        This tertiary treatment system uses gated irrigation pipe to disperse
     the secondary sewage effluent across and through the upper layers of this
     black spruce/tamarack peat bog.  Effluent is discharged to the bog in
     100,000 gallon slugs based on climatic and bog hydrologic conditions dur-
     ing the ice-free period of the year.
        Research on this system is based in four critical areas. Primarily,
     water quality changes are studied as the effluent moves from the secondary
     treatment lagoons, through the bog, and downstream to the Long Lake Branch.
     A second study of the productivity and mineral content of bog plants is

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     being made to determine how the bog species react to the application of the
     sewage effluent.  A companion study uses aerial and ground level stereo
     photography to study bog vegetation compositional changes due to effluent
     application.  A third study will compare the density and diversity of popu-
     lation of reptiles, amphibians, birds, small mammals, and terrestrial
     invertebrates between the project area and a nearby control area.  Finally,
     a water/nutrient budget will be developed combining bog hydrologic data and
     the above mentioned nutrient studies to determine how the bog reacts to
     these discharges.
454. Kappel, W.M.  1979b.  The Drummond Project: Applying Sewage Lagoon Effluent
        to a Bog:  an Operational Trial, pp. 83-89.  In; R.K. Bastian and S.C.
        Reed (eds.), Aquaculture Systems for Wastewater Treatment: Seminar Pro-
        ceedings and Engineering Assessment.  U.S. Environmental Protection
        Agency, Office of Water Program Operations, Municipal Construction
        Division Washington, DC. EPA 430/9-80-006.

        Sewage effluent was introduced into this 10 ha spruce-sphagnum bog in
     northwestern Wisconsin in May 1979.  Effluent was applied to the wetland
     surface twice each year (spring and autumn) using a gated irrigation pipe
     system.  This report contains a description of the overall operation of
     the system and the studies being conducted at the site on water quality,
     vegetation, and animal populations.  A water and nutrient budget study
     also has been initiated.  (EP)
455. Kardos, L. T., and J. E. Hook.  1976.  Phosphorus Balance in Sewage
        Effluent Treated Soils.  J. Environ. Qual. 5:87-90.

        Four field sites which have been irrigated with various levels of sec-
     ondary treated sewage effluent for 9 to 11 years have been examined for
     phosphorus content in soil water extracted with suction lysimeters.  The
     cornfield site on Hublersburg clay loam irrigated with effluent had an
     average P concentration of 0.329, 0.070, and 0.046 mg/liter in soil water
     at 15, 60, and 120 cm respectively compared to 0.043, 0.046, and 0.037
     mg/liter, respectively, under an adjacent fertilized, but unirrigated con-
     trol.  Year round application of effluent including 2 years of effluent
     plus sludge to reed canarygrass (Phalaris arjandinacea L.) on Hublersburg
     clay loam resulted in average soil water concentrations of 0.170, 0.100,
     and 0.052 mg/P/liter at 15, 60, and 120 cm, respectively.
        In an abandoned field on Hublersburg clay loam where no P was removed
     by crops, P concentrations were 0.140, 0.103, and 0.0765 mg P/liter for
     the treated plot and 0.038, 0.047 and 0.042 mg P/liter for the control at
     15, 60, and 120 cm, respectivley.  Only the 15-cm depth had significantly
     higher P concentrations in the treated plot.  A hardwood forest located on
     a Morrison sandy loam soil was irrigated year round. Phosphorus concentra-
     tions in soil water were 0.349, 0.080, and 0.087 mg/liter for the treated
     vs. 0.059, 0.039, and 0.039 for the control at 15, 60, and 120 cm, respec-
     tively.  Leaching losses were calculated from concentration of P in soil
     water at 120 cm and from leaching volume which was rainfall plus irrigation
     minus potential evapotranspiration.  Over all years of treatment no site
     had leaching losses of >3% of the total applied.  (AA)

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456. Kardos, L.T., W.E. Sopper, E.A. Myers,, R.R. Parizek, and J.B. Nesbitt.
        1974.  Ord. U.S. EPA

        A 500,000 gallon per day supply of chlorinated, secondary treated
     wastewater was applied through sprinkler irrigation systems to a combined
     acreage of approximately 70 acres of cropland and forestland in variously
     located experimental plots on Hublersburg silt loam or clay loam soils or
     on Morrison sandy loam soil.  The hydraulic loads tested ranged from one
     to six inches at weekly intervals.  The effects of application of the
     wastewater to the land on crop yields and crop chemical composition were
     generally favorable for the two hydraulic loads tested, 1 and 2 inches at
     weekly intervals during the growing season, only, or year-round. Harvested
     crops removed important amounts of the two key eutropic nutrients, phos-
     phorus and nitrogen.  On the forested sites tree growth respons was bene-
     ficial except with red pine on the Hublersburg soil when 2 inches of
     wastewater was applied at weekly intervals during the growing season.  N
     and P content of the harvested crops and forest foliage was generally in-
     creased by wastewater irrigation.
        Nitrate nitrogen levels in water recharging through the soil at the
     four foot depth were less than 10 mg/1 except in the growing-season-2-inch
     red pine on Hublersburg soil and the year-round-2-inch hardwood area on
     Morrison sandy loam soil when NO3~N values were 2 to 4 times the
     U.S.P.H.S. limit.
        Phosphorus concentration in the suction lysimeter water samples at a
     depth of four feet was no greater on wastewater treated areas than on un-
     treated control areas even on the sandy loam soil.  Passage through four
     feet of soil decreased P concentration 98 to 99% through the combined
     effects of soil and vegetation in the "living filter" system.  Deep well
     samples adjacent to the Hublersburg soil area showed no changes in water
     quality from normal background values.  One deep well completely surround-
     ed by wastewater treated sandy loam soil showed higher N03 and CL~
     concentrations in the fifth year of treatment but sill met U.S.P.H.S.
     drinking water standards.
        Recharge benefit to two nearby University water supply wells was esti-
     mated to result in water table build-up equivalent to 2 to 6 feet after a
     2 year period of recharge of 6.5 x 10' gallons per year on 43.5 acres.
        Annual costs of a spray irrigation system to recycle wastewater through
     the land was estimated to range from $13 per year per E.D.U. for 1 MGD to
     $81 per year per E.D.U. for 10 MGD.
        This report was submitted in fulfillment of Project No. 16080 DYJ under
     the partial sponsorship of the Water Quality Office, Environmental
     Protection Agency.
457. Katzenelson, E., and S. Kedmi, 1979.  Unsuitability of Polio Viruses  as
        Indicators of Virological Quality of Water.  Applied Environmental
        Microbiology 37.
458. Keating, K.I.  1976.  Interference by Blue-green Algae with Nutrient
        Recovery in Water-quality Control Schema:  Management Implications,  pp.
        241-245.  In; J. Tourbier and R.W. Pierson (eds.), Biological Control
        of Water Pollution.  Univ. of Pennsylvania Press.

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        Nutrient-rich natural waters are usually dominated by blue-green algae.
     From the viewpoint of a food chain culminating in harvestable fish the
     blue-greens are among the least desirable primary producers in that most,
     or all, are considered unsatisfactory food sources for zooplankton.  They
     become even less suitable for intentional cultivation when certain of
     their other characteristics are considered-e.g. many produce unpleasant
     odors and tastes in waters; some act as allergens, or toxins-for animals
     ranging from invertebrates to humans; some have recently been implicated
     as possible vectors for viruses, although the capacity to harbor viruses
     may provide a means to control blue-green algae.  It is therefore essen-
     tial develop the management information and techniques necessary either to
     control, or to eliminate, the blue-green algae.
        However, there is strong evidence that the competitive advantage of the
     blue-green algae is based at least in part on an allelopathic capacity.
     (AA)
459. Keenan, C.  1979.  Effect of a Desert Marsh System on the Fertility of
        Secondarily Treated Sewage Effluent Discharged into Lake Mead.
        Journal of the Arizona-Nevada Academy of Science, Vol. 14.   1979
        Proceedings Supplement.  Arizona State University, Tempe, AZ.
460. Keeney, D. R.  1973.  The Nitrogen Cycle in Sediment-water Systems.  J.
        Environ. Qual. 2:15-29.

        The available literature on the fate of nitrogen in waters and  sedi-
     ments is reviewed.  Emphasis is placed on the importance of N to aquatic
     productivity, the pathways leading to N gains or losses in aquatic eco-
     systems, and the availability of N in sediments to the overlying waters.
     Important biological reactions include N mineralization and immobiliza-
     tion, nitrification and denitrification, and N fixation.  The effect of
     sediment properties, lake morphology and environmental factors  (pH,
     temperature, dissolved oxygen, oxidation-reduction potential) on the
     pathways and rates of N turnover are considered.  The mixing process in
     sediments appear to be the most important in releasing sediment-N  to
     waters.  Several facets of the N cycle in waters and sediments  require
     further elucidation.  Research needs are outlined.
461. Keeney, D. R., and R. E. Wildung.  1977.  Chemical Properties of  Soils
        in:  Soils for Management of Organic Wastes and Wastewater.  Soil
        Science Society of America.  Madison, WI.
462. Kelley, R., and M.A. Harwell.  1983.  Comparisons of Processions of
        Elements by Ecosystems: Nutrients (Draft Manuscript) In; Ecological
        Considerations in Wetlands Treatment of Municipal Wastewaters.  Pro-
        ceedings of a Workshop, 23-25 June 1982, University of Massachusetts,
        Amherst, MA.  U.S. Fish and Wildlife Service and U.S. Environmental
        Protection Agency.

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463. Kemp, M. C., D. S. Filip, and D. B. George.  1978.  Evaluation and Com-
        parison of Overland Flow and Slow Rate Systems to Upgrade Secondary
        Wastewater Lagoon Effluent. Rept. No. Q-78-02. Utah Water Research
        Lab. Logan, UT.  70 pp.

        To evaluate the effectiveness of overland flow treatment in upgrading
     secondary wastewater lagoon effluent, three 15 x 36 m plots on a 2.5
     percent slope were constructed and sown for a high density vegetative
     cover using Reed Canary grass.  Wastewater was applied to the upper end
     of each plot at rates of 7.5, 15, and 22.5 cm/week.  Results from the
     overland flow system investigation were compared with similar data ob-
     tained the preceding year from an existing slow rate land application
     system on an adjacent site.  Secondary effluent from the same lagoon
     system as applied to the slow rate system study water quality character-
     istics from both systems, site specific efficiencies were detailed.  (NT)
464. Kerfoot, W. B., and S. A. Jacobs.  1974.  Permissible Levels of Heavy
        Metals in Secondary Effluent for Use in a Combined Sewage Treat-
        ment-Marine Aquaculture System I.  Monitoring During Pilot Operation,
        pp. 65-78.  In Wastewater Use in the Production of Food and Fiber
        Proc.  U. S. Environmental Protection Agency.  Ada, OK.
465. Kerfoot, W. B., and 6. A. Redmann.  1974.  Permissible Levels of Heavy
        Metals in Secondary Effluent for Use in a Combined Sewage Treat-
        ment-Marine Aquaculture System II.  Development of Guidelines by
        Method of Additions, pp. 79-101.  In Wastewater Use in the Production
        of Food and Fiber-Proc. U. S. Environmental Protection Agency.  Ada,
        OK.
466. Khalid, R. A., R. P. Gambrell, M. G. Verloo, and W. H. Patrick, Jr. 1977.
        Transformation of Heavy Metals and Plant Nutrients in Dredged Sedi-
        ments as Affected by Oxidation Reduction Potential and pH.  Vol. I:
        Literature Review.  Contract Report D-77-4.  U.S. Army Corps of
        Engineers Waterways Experiment Station, Vicksburg, MS.

        The occurrence and chemistry of selected trace metals and plant nutri-
     ents in sediment-water systems were studied.  The toxic and nutrient
     elements included in the study were Pb, Cd, Hg, As, Se, Cu, Zn, Mn, Fe, N,
     P, and S.  Effects of pH and oxidation-reduction conditions on metal and
     nutrient chemistry were stressed.  (AA)
467. Khalid, R. A., W. H. Patrick, Jr., and R. D. Delaune.  1977.  Phosphorus
        Sorption Characteristics of Flooded Soils.  Soil Science Society of
        Amer. Journal 41:305-310.

        Surface soils representing Alfisols and Inceptisols were collected from
     various parts of Louisiana under rice (pryza sativa L.) cultivation and
     incubated under oxidized (aerobic) and reduced (anaerobic)) conditions for

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     2 weeks in a soil to 0.01M CaCl2  solution ratio of 1:5.   The release of
     native soil P and the  sorption  of added  inorganic P was  investigated under
     these conditions.  The soils  selected for study were characterized for
     clay content, total carbon, extractable  P, pH,  and oxalate-extractable Fe,
     soil properties associated with P sorption.   The results show that
     generally more soil P  was released under reduced than oxidized conditions,
     and this increase in soluble  P  under  reduced conditions  was  significantly
     related to Bray No. 2  extractable P,  clay content, and oxalate Fe.   At
     high levels of added P, more  P  was sorbed under reduced  conditions than
     under oxidized conditions in  14 out of 20 soils under study.  However, in
     Midland fine sandy loam essentially all  of added P was recovered in the
     equilibrium solution under both oxidized and reduced conditions, suggest-
     ing this soil had no capacity to  sorb P.  The relationship between P
     sorbed at 500 ug/g added P and  oxalate-extractable Fe was described by
     fitting two linear curves with  different slopes.   In the reduced soil
     samples the slope of Region I (3,000  ug  Fe/g) suggests that  5.4 Fe sorbed
     1P and that this ratio did not  change until  all of the added P was sorbed.
     In the oxidized samples this  ratio was about 16 Fe/lP for Region 1( 4,500
     ug Fe/g).  The slopes  for Region  II of the linear curves for both reduced
     and oxidized samples were essentially zero.   A  closer relationship between
     P sorbed and oxalate extractable  Fe under reduced conditions indicates
     that poorly crystalline and amorphous oxides and hydroxides  of Fe play a
     primary role in P retention by  flooded soils and sediments.   A probable
     greater surface area generated  by the transformation of  oxyferric
     hydroxide to more reactive ferrous compounds may be responsible for
     greater P sorption under reduced  conditions.  (AA)
468. Kidlow, J., and J. E. Huguenin.   1974.  Problems  and Potentials  of
        Recycling Wastes for Aquaculture.  Mass. Inst. of Technology,  Sea
        Grant Program, 74-27. Cambridge, MA.

        The potentialities and problems of using thermal effluents  and/or
     secondary  sewage as inputs to marine aquaculture  systems  are discussed.
     Although marine aquaculture technology is relatively undeveloped, and  the
     use of waste in such a system is  even less developed, potential  for  such
     systems appears enormous.  In the future, the growing demand on  coastal
     zones for  both waste disposal and food production can be  met only by a
     sophisticated, well-managed system.  Feed cost for raising  fish  and  farm
     animals will probably continue to rise, making the use of recycled wastes
     for feed in an aquaculture process an economically attractive  concept.
     Research on the safety of marine waste-food recycling may have to be
     government sponsored.  Also, the economic viability of these systems has
     not been demonstrated.  (EL)
469. Kiemerle, R. A., and W. R. Enns.  1968.  Aquatic Insects Associated with
        Mid-western Waste Stabilization Lagoons.  J. Water Pollut. Control Fed.
        40:R31-R41.

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470. King, D. L.  1978.  The Role of Ponds in Land Treatment of Wastewater,
        pp. 191-198.  In State of Knowledge in Land Treatment of Wastewater.
        Vol. 2.  U. S. Army Corps of Engineers Cold Region Res. Eng. Lab.
        Hanover, NH.

        Ponds used for storage or pretreatment of wastewater prior to terres-
     trial application significantly alter wastewater quality.  Data from the
     Michigan State University Water Quality Management Facility (WQMF) indi-
     cate that significant permanent phosphorus reduction decreases markedly
     once benthic sediments are saturated with phosphorus but that excellent
     nitrogen removal continues.
        Phosphorus concentrations within such ponds vary seasonally but signif-
     icant permanent phosphorus removal should not be anticipated beyond the
     first two or three years of operation.  Harvest of aquatic plants yields
     only about 10% removal of both phosphorus and nitrogen.
        In wastewater ponds, aquatic plant growth leads to accelerated denitri-
     fication and increases pH to 10 or more causing large ammonia losses to
     the air during those periods of the year when algae and aquatic macro-
     phytes are active.  While this nitrogen loss to the atmosphere will be
     controlled by site-specific conditions of light availability and tempera-
     ture and alkalinity of the wastewater, nitrogen concentration decreases in
     a logistic fashion as a function of detention time within the WQMF ponds
     yielding a 95% nitrogen reduction in 120 days.  The rate of nitrogen
     removal, as gN/M2/day, from these ponds is a linear function of nitrogen
     concentration.
        In addition to marked nitrogen losses associated with aquatic plant
     growth, the ability of these plants to elevate the pH also leads to pre-
     cipitation of a variety of heavy metals.  Such alterations in wastewater
     should be included in design criteria whenever ponds are incorporated in
     land treatment of wastewater. (AA)
471. King, D. L.  1979.  Some Ecological Limits to the Use of Alternative
        Systems for Wastewater Management, pp. 299-307.  In R. K. Bastian and
        S. C. Reed (eds.), Aquaculture Systems for Wastewater Treatment:
        Seminar Proceedings and Engineering Assessment. EPA 430/9-80-006.
        U.S. Environmental Protection Agency, Office of Water Program Oper-
        ations, Municipal Construction Division, Washington, DC.

        A great variety of biological, chemical, and physical factors interact
     and feedback to set limits on the ability of natural ecosystems to process
     wastewater.  An understanding of these ecological limits allows better de-
     sign of alternative wastewater management systems which can be tailored to
     fit local environmental and wastewater conditions relative to local waste-
     water standards.  Ecological interactions responsible for oxygen supply
     for BOD satisfaction, plant production, and nutrient removal and recycle
     by alternative wastewater systems are considered for aquatic ecosystems
     and combinations of aquatic and terrestrial ecosystems. (AA)
472. King, D. L., and T. M. Burton.  1979.  A Combination of Aquatic and Ter-
        restrial Ecosystems for Maximal Reuse of Domestic Wastewater, pp.
        714-726. In American Water Works Association Research Foundation,

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        Proceedings of  the Symposium Vol.  1  held 25  - 30  March 1979 in Wash-
        ington, DC.

        In the design of pond-land wastewater processing  systems the best
     features of the ponds should be meshed  with the best features of the land
     to yield a system  which  meets specific  needs for wastewater management and
     reuse relative to  soil type, terrestrial vegetative  cover and climate of
     the individual region.
        While the nitrogen stripping capacity of ponds allows wastewater appli-
     cation to land on  which  the vegetative  cover is inefficient at nitrogen
     removal', it can limit both attempts to  reuse wastewater nutrients and
     attempts to maintain harvestable  fish populations in the ponds.   Where the
     goal is to maximize wastewater  nitrogen recycle through terrestrial crops,
     ponding of wastewater should be minimized,  particularly during the summer
     months, to prevent nitrogen loss  prior  to land  application.  When waste-
     water is stored over the winter and applied to  the land during the summer,
     considerable nitrogen loss will occur as a function  of  detention time in
     ponds, particularly during the  warmer months of the  year.  Nitrogen
     limitation of aquatic plants to types which cannot be harvested allows
     accumulation of significant BOD which,  when exerted,  is detrimental to any
     fish in the pond.  (AA)
473. Kitchens, W.M., Jr., J.M. Dean, L.H.  Stevenson,  and  J.H.  Cooper,  1975.  The
        Santee Swamp as a Nutrient Sink, pp.  349-366.   In;  J.B.  Gentry,  and
        M.H. Smith  (eds.), Mineral Cycling in Southeastern  Ecosystems. ERDA
        Symposium Series Conference -  740513.

        The Upper Santee Swamp, South  Carolina,  extending from the  confluence
     of the Congaree and Wateree Rivers, was  studied  for  its role in the reduc-
     tion of nutrient loads and fecal  coliforms.  The two rivers are heavily
     polluted from  sewage discharges and agricultural runoff but Lake Marion,
     an impoundment five miles downstream  of  the Rivers'  confluence has  not
     shown eutrophication symptoms typical  of shallow impoundments  receiving
     comparable nutrient loads.  The general  trend  observed, for both total  and
     reactive phosphates, a 50% reduction  in  concentrations as the  water passed
     from the rivers to the lower swamp provinces,  depending on flow.  Nitrate
     concentrations, consistently high in  both rivers,  was  essentially unalter-
     ed in passage through the swamp.  The flow  through the swamp during the
     study period was usually a mass sheet  over  the floodplain floor, with no
     additional stream runoff inputs.  Therefore concentration changes are
     definitely not due to dilution.  The  nutrient  sink is  considered primarily
     biological, with possible total phosphates  adsorbed  to suspended silts  and
     clays. There was also a significant reduction  in bacterial  counts,
     including fecal coliforms, with little or no oxygen  depletion  as the
     waters coursed over the swamp.
474. Klafter, R.D., and W.E. Mattis.  Optimal Waste Discharge  in Estuaries  and
        Bays.  Presented at the IFAC Congress.  Paris.  June 12-17,  1972.

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475. Klein, R.L., Jr.  1976.  The Fate of Heavy Metals  in Sewage Effluent
        Applied to Cypress Wetlands.  Master's Thesis,  Department  of  Environ-
        mental Engineering Sciences, University of Florida, Gainesville.
        151 pp.
476. Klemetson, S. L., and G. L. Rodgers.   1981a. Evaluation  of Biofilter Water
        Reuse Systems for Macrobrachium rosenbergii Prawn Aquaculture Ponds.
        Presented at the Water Reuse Symposium II.  Washington, DC.
        August 23-28.

        The yield of food protein from domestic aquaculture and its  relative
     importance as a food source of human nutrition is rapidly rising.   The
     interest in the commercial culture of  Malaysian prawns,  Macrobrachium
     rosenbergii, is steadily moving in the direction of intensification and
     the use of industrial methods.  Additionally, since the  prawn is a  warm
     weather animal which requires a temperature of approximately 87°F  (30°C),
     efforts are being made to protect the  pond surface and maintain the proper
     temperature profile.
        Water quality maintenance is the primary technical factor limiting the
     intensification of closed system aquaculture.  Good water quality can
     theoretically be maintained by pass through biological filters  where
     bacterial polycultures oxidize the toxic wastes, rendering it fit for
     reuse.  The nitrogenous wastes are the most important task of the
     biofilters.  The biofliters must operate in conditions that vary zero to
     12 ppt of salinity, and with temperatures near 87°F (30°c).
        The research on the treatment of fresh and saline wastewaters with a
     rotating biofilter has been completed, and the systems have produced good
     removal efficiencies using synthetic wastewaters for aquaculture pond
     wastewaters.  Current studies involve  live juvenile and  adult prawn
     connected to the biofilter system.  Concurrently, nitrogen compound
     toxicity studies are being conducted.  The adult toxic limit is approx-
     imately 26 mg/1 total available nitrogen, however, the biofilter systems
     can achieve effluent levels for biofilter reuse system at approximately
     1 mg/1.
        Current research is being conducted in the area of packed biological
     towers for both nitrification and denitrification of the heated, fresh
     and saline wastewaters produced by the live prawn aquaculture systems.
        The paper will compare the cost and efficiency of several types  of
     biological filter systems for direct aquaculture water reuse.   (AA)
477. Klemetson, S.L. and G.L. Rodgers.   1981b. Biological  Filters.   J.  Water
        Pollut. Control Fed.  53(6):687-699.
478. Klock, J. W.  1971.  Survival of Coliform Bacteria  in Wastewater Treatment
        Lagoons.  J. Water Pollut. Control Fed. 43:2071-2083.

        The survival of coliform  organisms in  domestic wastewater was studied
     under field conditions using a  specially  constructed channeled lagoon
     4,000 ft.  (1,218 m) long.  The  lagoon was continuously  fed with partially

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     settled raw wastewater, and the sequence of physical, chemical, and bio-
     logical events comprising the transformation of raw waste to a treated
     wastewater was studied in conjunction with the measurement of coliform
     survival.  Coliform die-away was observed to be of simple exponential
     form, N-^NolO^, where N is the number of organisms at times 0 and
     t and k is a rate constant.  The influence on k of temperature (T),
     oxidation-reduction potential (Eh), and pH was evaluated.  (AA)
479. Klopatek, J. M.  1974.  Production of Emergent Macrophytes and their Role
        in Mineral Cycling within a Freshwater Harsh.  Master's Thesis.
        Department of Botany, Univ. of Wisconsin.  Milwaukee.  278 pp.
480. Klopatek, J. M.  1975.  The Role of Emergent Macrophytes in Mineral
        Cycling in a Freshwater Marsh, pp. 367-393.  In F. G. Howell, J. B.
        Gentry and M. H. Smith (eds.), Mineral Cycling in Southeastern Eco-
        systems.  U.S. Energy Research and Development Administration Sym-
        posium Series (CONF-740513).

        Thersa Marsh, Wisconsin, was investigated by examining both the onto-
     geny of the species and their patterns of nutrient uptake and release.
     Three variable linear regression equations were employed to compare the
     changes in the amount of nutrients (G/SQ M) in the belowground and the
     aboveground standing crops and the level of soil nutrients in monotypic
     stands of tyjpha latifolia, scirpus fluviatilis, and carex lacustris. The
     highly significant correlation values between both scirpus and carex  (and
     to a lesser extent typha) and total soil nitrogen was somewhat surprising
     since, in continually waterlogged soils, ammonia is regarded as the sole
     plant-available nitrogen.  The magnitude of correlations with phosphorus
     indicated that the emergent macrophytes are perhaps the factor controlling
     the flux of phosphorus in the soil during the growing season. Total nitro-
     gen and available phosphorus in the marsh soil appear to be adequate; the
     macrophytes are acquiring their nutrients from the soil, and control the
     nutrients within the soil during the growing season.  Denitrification
     reactions appeared to occur throughout the year, especially during summer.
     Potassium, calcium, and magnesium concentratins throughout the year
     appeared dependent on the macrophyte uptake and leaching losses, total
     respiration in the marsh, and the physical and chemical characteristics of
     the water.
481. Klopatek, J.  1978.  Nutrient Dynamics of a Freshwater Riyerine Marsh  and
        the Role of Emergent Macrophytes, pp. 195-216.  In; R.E. Good, D.F.
        Whigham and R.L. Simpson  (eds.), Freshwater Wetlands: Ecological Pro-
        cesses and Management Potential.  Academic Press, New York.

        The open characteristic of freshwater riverine marshes  results in a
     combined subsidy and withdrawal of nutrients, with the specific patterns
     in a given wetland being dependent on seasonal hydrological fluctuations
     and biological activity.  A  key feature of the marsh separating it from
     terrestrial ecosystems is its inundated and anaerobic soils which show

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     statistically significant (P<.01) seasonal variations in available P and K
     and exchangeable Ca and Mg.  Based primarily on investigations in Wiscon-
     sin, the role of emergent macrophytes within the marsh nutrient cycle is
     described. Concentrations of N,P,K,Ca, and Mg are shown to follow predict-
     able trends over the macrophyte growing season.  Regression analyses
     revealed nutrient update by the macrophytes was significantly correlated
     (r2 = .98,P<.01) with total soil N and available P.  For the most part,
     significant correlations were not present for the other elements.  This is
     explained by their mobility and the possibility of luxury uptake.  Models
     of the flow of nutrients in a Scirpus fluyiatilis stand are depicted.  The
     effects of a marsh drawdown on the nutrient cycle and nutrient dynamics of
     Salix interior, a typical riverine marsh shurb, are also discussed.  (AA)
482. Knighton, M.D., and D.E. Streblow.  1979.  Study Plan:  Changes in a Bog
        Community Following Application of a Secondary Lagoon Effluent. Unpub-
        lished report.  U.S. Department of Agriculture, Forest Service, North
        Central Forest Experiment Station, Grand Rapids, MI.  11 pp.

        The research planned at this site includes measurements of species
     composition changes that may occur as a result of wastewater applications.
     Field methods such as low and high level photography of permanent quad-
     rats, ground truthing, vegetation mapping, and other techniques are des-
     cribed.
483. Kok, T.  1974.  The Purification of Sewage from a Camping Site with the
        Aid of a Bulrush Pond.  H20 7(24):536-544.
484. Konefes, J.L., and R.W. Bachman.  1970.  Growth of the Fathead Minnow
        (Pimepha1es promelas) in Tertiary Treatment Ponds.  Iowa Acad. Sci.,
        77:104-111.
485. Kothandarman, V., and R.L. Evans. 1972.  Removal of Algae from Waste Sta-
        bilization Pond Effluents.  State of the ARt. 111. Water Surv. Circ.,
        108 pp.
486. Kowal, N.E., and H.R. Pahren.  1981. Health Effects Associated with Waste-
        water Treatment and Disposal.  J. Water Pollut. Control Fed. 52(6):
        1312-1325.
487. Kramer, J.R., S.E. Herbes, and H.E. Allen.  1972. Phosphorus Analysis  of
        Water, Biomass and Sediment, pp. 51-100.  Ii» H.E. Allen and J.R. Kramer
        (eds.), Nutrients in Natural Waters.  John Wiley and Sons, New York.

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488. Krapu, G. L., D. R. Parsons, and M. W. Waller.  1970.  Waterfowl in
        Relation to Land Use and Water Levels on the Spring Run Area.  Iowa
        State Journal of Science 44:437-452.
489. Krauss, A. P.  1979.  Design Involvement Leads to Public Acceptance.
        Water and Wastes Engineering 16( 11):50-52.

        The design and construction of the new advanced wastewater treatment
     plant at Greenville, Maine, is described with tables and diagrams.  The
     facility uses the Marsh Farm land application system for wastewater
     treatment and disposal, and comprises aerobic/facultative treatment of
     wastewater to a secondary level, followed by storage in a lagoon system
     ready for spray irrigation from May to November.  (AL)
490. Krebs, C. T., and I. Valiela.  1978.  Effects of Experimentally Applied
        Chlorinated Hydrocarbons on the Biomass of the Fiddler Crab, Uca.
        pugnax. Estuar. Coast. Mar. Sci.  6:375-386.
491. Krebs, C. T., I. Valiela, G. Harvey, and J. M. Teal.  1974.  Reduction of
        Field Populations of Fiddler Crabs by Uptake of Chlorinated Hydro-
        carbons. Mar. Pollut. Bull. 5:140-142.
492. Krishnamoorthi, K. P., and M. K. Abdulappa.  Domestic Wastewater Utiliza-
        tion Through Aquaculture Studies at Nagpur, India.  Presented at IAWPR
        Developments in Land Methods of Wastewater Treatment and Utilization
        Intl. Conf. Melbourne, Australia.  Oct. 23-27, 1978.

        In Nagpur City, India, domestic sewage is being treated in stabiliza-
     tion ponds.  A four year study was conducted to examine algal ecology,
     productivity, and operational and biological parameters in the system.
     Results indicated that the system achieved a very large reduction in
     pathogenic load, recycled domestic wastewaters efficiently, and yielded
     many useful by-products.  The relative low cost of stabilization ponds
     makes them a viable economic proposition.  (EL)
493. Krull, J.N. 1970.  Aquatic Plant Macroinvertebrate Associations and Water-
        fowl.  Journal of Wildlife Management  34(4):707-718.
494. Krupauer, V.  1971.  The Use of Herbivorous Fishes for Ameliorative
        Purposes in Central and Eastern Europe, pp. 95-103.  In Proc. Eur.
        Weed Res. Counc. 3rd Int. Symp. Aquat. Weeds.

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495. Kruse, H.  1962.  Some Present-day Sewage-treatment Methods in Use for
        Small Communities in the Federal Republic of Germany.  Bull, of the
        World Health Organization 26:542-549.
496. Kruzic, A. P.  1979.  Water Hyacinth Wastewater Treatment System at
        Disney World, pp. 257-272.  In R. K. Bastian and S. C. Reed  (eds.),
        Aquaculture Systems for Wastewater Treatment: Seminar Proceedings and
        Engineering Assessment.  EPA 430/9-80-006.  U. S. Environmental Pro-
        tection Agency, Office of Water Program Operations, Municipal Con-
        struction Division. Washington, DC.

        In general all of the components of the project are operating as
     planned but there have been problems.  For example, when the ponds were
     first seeded the hyacinths were hand picked and placed into the channels.
     This was too slow, so a backhoe was used to finish the seeding.  This was
     a mistake because a great deal of dollarweed and grass was brought in with
     the hyacinths.  Some of the unwanted species were removed but total segre-
     gation was not possible.  The unwanted plant species were harvested out of
     the system only after much effort.
        The only major concern at this time is whether or not the system can
     effectively handle primary effluent.  The dissolved oxygen concentrations
     are very low, floating sludge in the open areas has appeared and there are
     many fly larvae where the ponds once flourished with thousands of mosquito
     fish.  Fortunately mosquitoes have not been a problem so far.
        In summary, the hyacinth project at WALT DISNEY WORLD is still in the
     process of start up.  The scope of the project is quite large and there
     are many details to be worked out.  The system is unique in its attempt
     to provide all the data needed by engineers to design a water hyacinth
     wastewater treatment system.  (AA)
497. Kuenzler, E. J.  1971.  Aspects of Phosphorus Cycling in Brackish Waters.
        Univ. of North Carolina, Chapel Hill.  50 pp.   (NTIS ORO-3549-7)

        In this study the consequences of subjecting small brackish water  eco-
     systems to domestic sewage was investigated.  Six ponds, three receiving
     municipal sewage wastes, were regulated with seawater, freshwater and
     sewage to control salinities and retention time.  The ponds were seeded
     with larvae and microscopic plants, fish, and invertebrates.  Measure-
     ments of temperature, salinity, insolation, plant nutrients, standing
     crop, and metabolic rate were taken for a two year period.  The ponds
     acted as tertiary treatment or oxidation ponds and prevented the deteri-
     oration of the quality of receiving water.  The study indicates that
     reasonable amounts of sewage waste may not prove harmful to functioning
     brackish ecosystems.  (WE)
498. Kuenzler, E. J., and A. F. Chestnut.  1971.  Structure and Functioning  of
        Estuarine Ecosystems Exposed to Treated Sewage Wastes.  Institute  of
        Marine Sciences, Univ. of North Carolina, Morehead City, NC.   346  pp.

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        This is the third annual report from an investigation of the eco-
     logical systems which develop when estuarine waters are enriched with
     sewage wastes*  Faculty and students from the University of North
     Carolina have studied various phases of community structure and
     metabolism of six experimental brackish water ponds, three of which
     receive treated sewage wastes, and of a small tidal creek and its salt
     marshes.  In this report are chapters on productivity, carbon metabolism,
     the phosphorus budget, nitrogen, and bacterial heterotrophy; on the
     standing crops of phytoplankton, decapod crustaceans, fishes , meiofauna,
     foraminifera, insects, molluscs, and birds; on calcium analysis; and on
     growth and reproduction of algae*  The waste ponds have developed into
     productive, well-integrated, but slightly unstable systems.  They perform
     some of the functions of tertiary treatment and hold promise for pro-
     duction of harvestable seafood protein.  (NT)
499. Kuenzler, E. J., W. Laughinghouse, J. A. Marsh, M. S. May, and H. N.
        McKellar.  1971.  Structure and Functioning of Estuarine Ecosystems
        Exposed to Treated Sewage Wastes.  II.  Inst. of Marine Sciences.
        Univ. of North Carolina.  Morehead City, NC.  53 pp.

        Contents:  Insolation, pH, and turbidity;  notes on hydrography and
     phytoplankton of Calico Creek; direct bacterial counts; micro-organism
     diversity study of the two sets of ponds at Morehead City, North Carol-
     ina; excretion of dissolved organic phosphorus by plankton.   (NT)
500. Kuenzler, E.J., A.F. Chestnut, and C.M. Weiss.  1973.  Structure and Func-
        tioning of Estuarine Ecosystems Exposed to Treated Sewage Wastes, III.
        1971-1972.  Sea Grant Rept.  No. 73-10. Univ. of North Carolina. Chapel
        Hill, NC.  228 pp.

        This is the fifth report of investigations of the ecological systems
     which develop in small brackish-water ponds enriched with secondarily
     treated domestic sewage.  Six ponds were built, three of which received
     sewage wastes from the town of Morehead City, North Carolina.  In essence,
     the waste ponds were tertiary treatment or oxidation ponds.  Brackish oxi-
     dation ponds have seldom been studied and consequently there was a need to
     examine, insofar as possible, the entire system, the physical factors, the
     water chemistry (especially plant nutrient), the plant and animal popula-
     tions which developed, the metabolic processes of the pond systems, the
     effects of the pond system on water quality, and the aquaculture poten-
     tials.  (NT)
501. Kuenzler, E.J., P.J. Mulholland, L.A. Ruley, R.P. Sniffen.  1977.  Water
        Quality in North Carolina Coastal Plain Streams and Effects of Channel-
        ization.  North Carolina Water Resources Research Institute, Raleigh,
        NC.  UNC-WRRI Rpt. No. 127.  NTIS No. PB-278 273. October.  160 p.

        The physical and chemical characteristics of seven small Coastal Plain
     streams of eastern North Carolina were studied.  Three natural streams
     which flow through relatively undisturbed bottomland hardwood forests were

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     compared to four streams which had been channelized  for  the purpose  of
     reducing agricultural losses caused by flooding.  Two or three  sampling
     stations were established on each stream.  Some stations on natural
     streams were essentially pristine but others received point-source inputs
     of livestock farm wastes.  The natural streams had broad floodplains and
     low stream velocities even during flood stages.  Spates occurred  in  all
     seasons, but high discharge, and concomitant flooding of natural  swamps,
     were usually greatest during winter and spring.  The waters of  channelized
     streams, however, were restricted: they attained higher velocities,
     carried greater particulate loads, and were more turbid than  natural
     streams.  Some channelized streams were enriched by  sewage, by  livestock
     wastes, or by agricultural fertilizer.  Weighted mean concentrations of
     the nutrient element N and P were much lower in waters in unpolluted nat-
     ural streams than in rainwater, and there was a net  input of  these
     nutrients to the watershed.  Concentrations and fluxes of elements such as
     Ca, Mg, Na, K, and Cl, however, were higher in all streams than in precip-
     itation and the net fluxes of these elements were directed out  of the
     watershed.
502. Kuo, S. and D.S. Mikkelsen.  1979.  Distribution  of  Iron  and Phosphorus in
        Flooded and Unflooded Soil Profiles and their  Relation to Phosphorus
        Absorption.  Soil Science 127:18-25.

        Phosphorus adsorption was investigated in two, paired  soil  profiles-
     flooded rice and irrigated pasture-to elucidate changes of phosphorus as a
     consequence of long term seasonal soil flooding.  In  the two concentrations
     studied, 10 and 50 ppm P, the adsorption of phosphorus was considerably
     higher in the flooded rice topsoils, 0 to 30 cm in depth,  than in the up-
     land topsoils.  The difference in phosphorus adsorption gradually
     decreased as soil depth increased.  High phosphorus  adsorption in the rice
     topsoils was attributed to the higher content  of  amorphous iron resulting
     from reduction caused by seasonal soil submergence.
        Fractionation of phosphorus in the soil profiles  revealed that prolong-
     ed seasonal flooding tends to increase the 0.1 N  NaOH-extractable phos-
     phorus and, concomitantly, to decrease the reductant soluble and calcium
     phosphate contents.  The lower pH and reductive conditions prevailing in
     the flooded rice soils appear to be responsible for  the transformations of
     calcium and crystalline iron phosphates to amorphous types of  phosphate
     precipitates.
        No significant eluviation, of iron or phosphorus was observed in the
     flooded rice, as compared with adjacent pasture soils.  The high clay con-
     tent of these soils, coupled with a virtual lack  of  water movement through
     the profiles, may restrict the movement of these  two elements.  (AA)
   _. Kusler, J.A.  1980.

   . Kusler, J.A.  1983.
503. Kutty, M.N., 1980.  Aquaculture  in Southeast Asia:  Some Points of Empha-
        sis.  Aquaculture.  20(3):159-168.

        Some aspects of aquiculture in Asia  are  reviewed,  with emphasis on the
     need for increased production of cheaper  animal protein.   The Eastern cul-
     tures have generally been more advanced in  the commercial growing of
     aquatic speciss.  Aquiculture is practiced  in developing countries mainly

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     to provide organisms to serve as food and to recycle organic wastes.   The
     priorities of current aquiculture programs, which are  supported by many
     international organizations, are discussed.  The use of  sewage as a  nutri-
     ent source in fish culture systems has been practiced  in several Asian
     countries.  Controlled eutrophication has given high production rates  with
     caged fish in Indonesian streams, and the polyculture  of carp has been
     tested in sewage-fed ponds in India.  Fish raised on sewage must be
     examined thoroughly for pathogens before they are deemed fit for human
     consumption.  Semi-cultural conditions of fish growing using oceans  and
     man-made reservoirs may also be profitable.  The abiotic factors affecting
     fish reproduction and growth must be carefully controlled.  Behaviorial
     patterns of fish should also be studied to optimize fish growth and pre-
     vent wasting of energy derived from food.  Other areas of aquiculture  that
     require further research are the genetic improvement "of  species used for
     breeding and fish food sources.
504. Lackey, J.B. 1967.  Nutrient and Pollutant Response of Estuarine Biotas.
        In; Proc. Natl. Symp. Estuarine Pollution.  Aug. 23-25,  1967.

        The behavior of microbiota, algae and protozoa, as affected  by  commer-
     cial, recreational, and metropolitan uses of estuarine waters are  out-
     lined.  Some of the findings include: 1) estuaries and bays  are of great
     economic and recreational value as nurseries, fishing grounds,  shellfish-
     eries, and boating and bathing areas, 2) human activities often modify  the
     preferred ecology by adding nutrients or pollutants, 3) the  suspended
     microscopic plants and animals (plankton), and those of the  interface
     (benthos) are most easily studied qualitatively and quantitatively,  4)
     some pollutants, such as silt, sharply reduce the microscopic populations,
     and 5) the role of the engineer in estuarine studies is seeing  that  ade-
     quate biological studies are made so adequate treatment facilities will
     prevent overenrichment or pollution.  (WE)
505. Lakshman, G. 1979.  An Ecosystem Approach to the Treatment of Waste
        Waters.  J. Environ. Qual. 8(3):353.

        Aquatic plants such as bulrush (Scirpus sjgp_) and cattail  (Typjha spp)
     were used to purify untreated municipal sewage in experimental tanks in  a
     growth chamber measuring 5.5 by 3.7 by 3.7 m high.  Initial  concentrations
     of total phosphorus (TP) and total Kjeldahl nitrogen  (TKN) before treat-
     ment ranged from 3.9 to 29.0 ppm for TP and from 10.3 to 44.0 ppm for TKN.
     Rates of removal of phosphorus and Kjeldahl nitrogen by the  plants were
     monitored and compared with a control without plants.  Generally, the
     concentrations of TP and TKN decreased exponentially in the  treatment
     trays during the individual experimental runs. High rates of purification,
     up to 98%, were achieved in <20 days.  During the 20 experimental runs
     lasting >500 days the plants showed unabated ability to remove nutrients
     while the control achieved a saturated condition.
        Rates of nutrient uptake by the experimental plants increased with the
     ambient concentration and were much faster than the rates predicted by
     Michaelis-Menten kinetics.  Above-water biomass obtained by  periodic har-
     vesting showed that the plants contained high levels of digestible organic

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     matter (DOM) and crude protein comparable to the values obtainable from
     alfalfa.  Experimental data have been used in develop equations for large-
     scale in situ applications.  Data presented here show that aquatic plants
     have considerable potential in the purification of municipal waste waters
     within reasonably short periods.
506. Lakshman, G.  1980.  A Demonstration Project at Humboldt to Provide Ter-
        tiary Treatment to the Municipal Effluent Using Aquatic Plants.  1979
        Progress Report.  Saskatchewan Research Council.  Saskatoon, Saskatche-
        wan, Canada, 67 pp.
507. Lalancette, J.M. , and B. Coupal.  1972.  Recovery of Mercury from Polluted
        Water through Peat Treatment, pp. 213-217.  in; Fourth Int. Peat Cong.
        Proc. Ontaniemi, Finland.
508. Lance, W.R.  1971.  Use of Tertiary Treated Water for Waterfowl Habitat
        Fish Rearing Facilities.  Master's Thesis.  Colorado State University.
509. Larsen, J.E., G.F. Warren, and R. Langston.  1959.  Effect of Iron, Alumi-
        num, and Humic Acid on Phosphorus Fixation by Organic Soils.  Soil Sci,
        Soc. Amer. Proc. 23:438-440.
510. Larson-Albers, C.E.  1981.  The Effects of Wetland and Drainage Ditch Out-
        puts on the Water Quality of a Stream in South Central Minnesota, pp.
        181-198.  In; B. Richardson (ed.), Selected Proceedings of the Midwest
        Conference on Wetland Values and Management. Minnesota Water Planning
        Board.  St. Paul.  660 pp.

        To form a baseline study of a proposed county ditch, nutrient levels
     (PO4-P, TKN and N03~N) were monitored during 1980 at eight sites along
     the stream proposed to be channelized.  Tributaries from three partially
     drained wetlands and four drainage ditches were also sampled to compare
     wetland and ditch outputs and to assess their impact on the stream's water
     quality.  The 4554-acre watershed of the stream was dissected into the
     tributary subsheds and percentage of different land use categories, num-
     bers of animal units, and tiling histories were compiled and examined for
     relationships to water chemistry differences.
        From the significant differences found in nutrient concentrations among
     sites, it was possible to form a wetland-to-ditch continuum bounded at
     either end by the most distinct wetland (P04-P, TKN, and NO3~N means
     of .919, 3.42, and 0.24 ppm) and the most distinct ditch (PO4~P, TKN,
     and NO3~N means of .112, 1.32, and 8.75 ppm).  In the spring, one wet-
     land of the three studied and two in the summer were significantly differ-
     ent from drainage ditches with higher levels of PO4~P and TKN and lower
     levels of NO3~N.  Differences among the other sites were based primarily

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     on nitrate levels in which the wetlands as a group could be separated by
     their lower concentrations from the ditches.  Land use possibly
     contributes to these differences, as a lower row crop-to-wetland ratio  in
     the watershed of a tributary corresponds to more wetland-like chemistry in
     the outlet.
511. Lazio, T.  1972.  Reeds Control Eutrophication of Balaton Lake.  Water
        Research 6:1533-1539.

        The effects of sewage from a water treatment plant on Lake Balaton,
     Austria, was studied.  A comparison was made of the effect of effluent
     that flowed through reeds versus the effluent that flowed directly  into
     the lake.  The effluent passing through the reeds had hardly detectable
     amounts of nitrogen and phosphorus.  (WE)
512. Lee, C.R., et. al., 1976.  Feasibility of the Functional Use of Vegetation
        to Filter, Dewater, and Remove Contaminants from Dredged Material. U.S.
        Army Corps of Engineers Environmental Laboratory, Tech. Rept. D-76-4
        NTIS No. AD A028336.
513. Lee, C.R., B.L. Folsom, Jr., and R.M. Engler.  1980.  Availability  and
        Plant Uptake of Heavy Metals from Contaminated Dredged Material  Placed
        in Flooded and Upland Disposal Environments.  U.S. Army Corps of Engi-
        neers Waterways Experiment Station, Vicksburg, MS.

        A solid-phase plant bioassay was used in an attempt to verify previous
     dredged material results and to develop a procedure that could indicate
     phytotoxicity and bioaccumulation of heavy metals in contaminated dredged
     material.  Sediments known to be highly contaminated with one or more con-
     taminants were collected from five freshwater locations in the Great Lakes
     and five saltwater locations in the U.S.  The plant species used as indi-
     cators were Cyperus esjpu^enjbjas in the freshwater sediment tests and Spar-
     tina alterniflpra and Di^Jbichlj.^ spicata in the saltwater sediment  tests.
     The bioassays indicated that greater uptake of Cd and Zn occurred from
     dredged freshwater sediments in the upland disposal environment than from
     the flooded environment.  The reverse was true for As, with an increase  in
     bioaccumulation and phytotoxicity observed in the flooded environment.
     Factors influencing the availability and plant uptake of the heavy  metals
     include sediment redox potential, organic matter content, total sulfur
     content, and pH.  (WR)
514. Lee, C., R. Hoeppel, G. Hunt, and C. Carlson.  1967.  Feasibility of  the
        Functional Use of Vegetation to Filter, Dewater and Remove Contamin-
        ants from Dredged Material.  Tech. Rept. D-76-4.  U.S. Army Corps  of
        Engineers Waterways Experiment Station, Vicksburg, MS.

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515. Lee, C.R., R.M. Smart, T.C. Sturgis, R.N. Gordon, Sr., and M.C. Landin.
        1978.  Prediction of Heavy Metal Uptake by Marsh Plants Based on Chemi-
        cal Extraction of Heavy Metals from Dredged Material.  Tech. Rept.
        D-78-6.  U.S. Army Corps of Engineers Waterways Experiment Station.
        Vicksburg, MS.  107 pp.

        Marsh plant species studied included Spartina a^terniflora, Spartina
     patens, and Distichlis spticata.  Results indicated that most marsh plants
     colonizing dredged material disposal sites sampled in this study contained
     relatively low concentrations of Zn, Cu, Cd, Pb, Cr, Ni, and Hg.  These
     concentrations were very similar to those reported for natural coastal
     marshes.  There were a few locations, however, in which the concentrations
     of Zn, Cu, Cd, and Pb in the marsh plants were an order of magnitude
     greater than the concentrations measured in the majority of marsh plants.
     The occurrence of these elevated concentrations of heavy metals emphasizes
     the need for a method to predict heavy metal availability from dredged
     material to plants.  (AA)
516. Lee, C.R., T.C. Sturgis, and M.C. Landin.  1976.  A Hydroponic Study of
        Heavy Metal Uptake by Selected Marsh Plant Species.  Tech. Rept.
        D-76-5.  U.S. Army Corps of Engineers Waterways Experiment Station,
        Vicksburg, MS.

        Eight species of marsh plants were grown in chemically controlled
     hydroponic solutions containing three concentrations of heavy metals to
     evaluate the ability of each plant species to take up and accumulate heavy
     metals.  The marsh plants were Cyperus esculentus, Scirpus validus, Spar-
     tina patens, Scirpus robust us, pistichlis spicata , Tric[lochin mar i t ima,
     Sjpjirtina a 11 er ni f lor a, and Spartina foliosa.  The heavy metals studied
     were Zn, Cd, Ni, Pb, and Cr, at concentrations of 0.0, 0.5, and  1.0 ppm.
     Marsh plants were exposed to heavy metals for six weeks and harvested.
     Plants were separated into tops, lower stems, rhizomes, tubers,  and roots
     and analyzed for heavy metals to locate plant parts where heavy  metals
     may accumulate.
        Exposure to heavy metals adversely affected the growth of jS.  validus,
     £• patens, D. spi cat a, and S_. alterniflora more than the other plant
     species evaluated.  The species that appeared to have more potential in
     taking up Zn, Cd, and Ni were C. e sculentus, jS. patens, D. spipata> and to
     some extent IS. alternif 1 or a.  Pb and Cr accumulated in the roots of all
     species with very little translocation into plant tops.  Phosphorus and
     iron content in the roots apopeared to be a major factor in determining
     the ability of a marsh plant to translocate heavy metals from the roots
     into other plant parts.  (AA)
517. Lee, G.F., E. Bentley, and R. Admundson. 1969. Effect of Marshes  on Water
        Quality.  Water Chemistry Laboratory, Madison, WI.  25 pp.
518. Lee, G.F. , E. Bentley, and R. Admundson. 1975.  Effects of Marshes  on
        Water Quality,  pp. 105-127.  In; A.D. Hasler  (ed.), Coupling  of Land

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        and Water Systems.  Ecological Studies, Vol.  10.  Springer-Verlag, New
        York, NY.

        Four marsh areas located near Madison, Wisconsin, were  studied  for
     several years to determine the effects of these wetlands on water  quality.
     Productivity, chemical composition, and nutrient transport from the marsh-
     es are described based on data from the sites.   It was determined  that
     marshes have both beneficial and detrimental effects on water  quality,  and
     that the beneficial effects outweigh the detrimental effects.  (EP)
519. Lee, G.F., and R.A. Jones.  1980.  An Approach for the'Evaluation  of Effi-
        cacy of Wetlands-based Phosphorus Control Programmes for Eutrophication
        Related Water Quality Improvement in Downstream Water Bodies.   Water,
        Air, and Soil Pollution 14:359-378.

        It has been suggested that re-creation of the marshes that  used to
     exist in the Okeechobee Lake, Florida, watershed is the key to the preven-
     tion of further eutrophication of this lake.  The need for information  on
     the factors controlling algae and other aquatic growth is stated,  and in-
     formation available on the subject is reviewed.  The cycling of the con-
     trolling element(s) through restored wetlands must also be considered,  and
     whether a Florida wetland is a significant nutrient trap.  The possibility
     of developing a sufficient acreage of wetlands to maintain and, where
     possible, improve water quality in Lake Okeechobee is discussed.   It is
     concluded that the proposed wetlands may have limited impact on eutrophi-
     cation-related water quality of the lake.  (AL)
520. Lee, K.W., R.J. Natuk, R.L. Simpson, and D.F. Whigham.   1977.  Effects pf
        Sewage on Soil Algae of a Freshwater Tidal Marsh.  J. of Phycology Vol.
        13 (supplement).  39 pp.
521. Leendertse, J.J.  1970.  Environmental Simulation as a Tool in a Marine
        Waste Disposal Study of Jamaica Bay.  Rept. No. P-4163.  Rand Corp.
        Santa Monica, CA.  11 pp.

        The study of technical solutions to the problem of managing fluid waste
     discharges in an estuary involves complicated relationships, such as those
     between the waste load, the location of discharges, the degree of treat-
     ment, the geometry of the estuary, the flow in the estuary, and the temp-
     erature.  Models can be built to predict the probable consequences of each
     of the alternative solutions chosen.  Using the predictions obtained from
     these models and whatever other information or insight is relevant, alter-
     natives can be compared and conclusions drawn concerning the most desir-
     able course of action.  Such a model for well-mixed estuaries and coastal
     seas is presented and described.  (NT)
522. Lefor, M.W., W.C. Kennard, and T.B. Helfgott (eds.).  1976.  Proceedings:
        Third Wetlands Conference.  The University of Connecticut Institute of
        Water Resources*  Report No. 26.  August 1976.

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        The work upon which this publication is based was supported in part by
     funds provided by the United States Department of the Interior, as author-
     ized under the Water Resources Research Act of 1964, Public Law 88-379.
523. LeFurgey, A., and J.St. Jean, Jr.  1976.  Foraminifera in Brackish-Water
        Ponds Designed for Waste Control and Aquaculture Studies in North
        Carolina.  Journal of Foraminiferal Research.  6(4):274-294.

        Effluent may not itself be lethal to foraminifera or deleterious to
     sedimentary environments but effluent in experimental ponds does not favor
     foraminifera success.  This study determines how sewage effluent affects
     foraminifera species composition and diversity, standing crops, and sea-
     sonal and aeral distribution in six artificial ponds on Calico Creek and
     Bogue Sound, North Carolina.  Each 0.1 acre pond with maximum depth less
     than one m, was constructed and began operation in late 1968; monthly sam-
     pling was initiated in fall 1970.  Bogue Sound ponds simulate conditions
     of a well-flushed shallow marine lagoon typical of U.S. mid-Atlantic
     coast.  Calico Creek ponds simulate a shallow marine marsh estuary pol-
     luted by secondary effluent discharged from a sewage treatment plant.
     Elphidium clayatum is the most common species in both effluent and control
     ponds.  Species diversity is 20% higher and average living-foraminifera
     numbers are five times greater than effluent ponds.  Standing crops in
     effluent ponds average 4.5 living specimens cc, in control ponds 23.7
     living specimens cc.  Estimated productivity is trebled in control ponds.
     Microenvironmental differences in oxygen, pH, and food supply make fora-
     miniferal distribution erratic.  Moderate summer phytoplankton blooms
     occur in control ponds. In effluent ponds, dense winter phytoplankton
     blooms and concurrently low light levels, devastate original foraminifera
     populations, creating fluctuating foraminifera ecological significance.
524. Leonard, R.A., Bailey, and Swank, R.R., Jr.  1976. Transport, Detoxifica-
        tion, Fate and Effects of Pesticides in Soil and Water Environments,
        pp. 48-78.  In; Land Application of Waste Materials, Soil Conservation
        Society of America, Ankeny, IA.
525. Lewis, B.C., P.C.  Chee, R.M. Goldstein, F.C. Kornegay, D.L. Mabes, L.F.
        Soholt, and W.S. Vinikour.  1978.  A Biologist's Manual for the Evalua-
        tion of Impacts of Coal-Fired Power Plants on Fish, Wildlife, and Their
        Habitats.  U.S. Fish and Wildlife Service.  FWS/OBS-78/75.  206 pp.
526.  Lewis, J.C.,  and E.W. Bunce (eds.).  1980.  Rehabilitation and Creation of
        Selected Coastal Habitats:   Proceedings of a Workshop, U.S. Fish and
        Wildlife Service.  FWS/OBS-80/27.  162 pp.

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527. Lewis, S.J.  1977.  Avian Communities and Habitats on Natural and Waste-
        water Irrigated Vegetation.  Master's Thesis.  Pennsylvania State
        University, University Park.
528. Lewis, W.M., and M. Bender.  1961.  Effect of a Cover of Duckweeds and the
        Alga Pijthophora Upon Dissolved Oxygen and Free Carbon Dioxide of Small
        Ponds.  Ecology 42:602-603.

        The investigation of ponds which had a cover of duckweeds revealed that
     oxygen was very low and carbon dioxide abnormally high.  The observations
     given indicate that a covering of duckweeds and to a lesser extent a cov-
     ering of Pithophora can reduce the dissolved oxygen of water to a critical
     level for fishes.  There is also an increase in free carbon dioxide.  A
     comparison between ponds with some cover and a pond without cover demon-
     strates a marked difference in these two variables.
        Although actual fish mortality was observed only once, (Pond 1, 1959)
     repopulation of Pond 1 by flooding occurred in the fall of 1959 and at the
     time of the August 1960 observations, the fishes were gasping at the
     surface.
529. Lin, S.Y.  1974.  The Dialectics of a Proposal on Biological Control of
        Eutrophication in Sewage Lagoons.  In; Proc. Conf. on Wastewater Use in
        the Production of Food and Fiber. U.S. Environmental Protection Agency.
        Ada, OK.
530. Linde, A.F.  1969.  Techniques for Wetland Management.  Wisconsin Depart-
        ment of Natural Resources Research Rept. 45.  Madison, WI.  155 pp.
531. Linde, A.F., T. Janisch, and D. Smith.  1976.  Cattail - The Significance
        of Its Growth, Phenology, and Carbohydrate Storage to Its Control and
        Management.  Tech. Bull. No. 94.  Wisconsin Department of Natural Re-
        sources.  Madison, WI.  27 pp.
532. Lindsley, D., T. Schuck, and F,. Stearns.  1976.  Productivity and Nutrient
        Content of Emergent Macrophytes in Two Wisconsin Marshes, pp. 53-76.
        In;  D.L. Tilton, R.H. Kadlec, and C.J. Richardson (eds.), Proceedings
        of a Symposium on Freshwater Wetlands and Sewage Effluent Disposal,
        Univ. of Michigan, Ann Arbor.

        This study took place at McNaughton Marsh, a relatively infertile marsh
     on the  Wisconsin River in north-central Wisconsin.  The purpose was to
     determine if aquatic emergent macrophytes influenced the level of avail-
     able soil nutrients and consequently water quality.  Data from this infer-
     tile marsh is compared to data from Theresa Marsh, a fertile marsh.  Con-
     clusions are that levels of nutrients in the sandy substrate appear to be

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     controlled to a great extent by vegetation.  McNaughton marsh appears to
     function in a similar manner to Theresa marsh with the major difference
     being the quantity of nutrients involved.  Authors suggest that sandy
     marshes, such as McNaughton, whose soils do not bind nutrients appreciably
     offer high promise for wastewater treatment.  (WE)
533.  Lindsley, D., T. Schuck, and F. Stearns.  1977.  Primary Productivity and
        Mineral Regimes in a Northern Wisconsin Marsh, pp. 38-52.  In; C.B.
        DeWitt and E. Soloway (eds.), Wetlands Ecology, Values, and Impacts.
        Proceedings of the Waubesa Conference on Wetlands held 2-5 June 1977.
        Institute for Environmental Studies, University of Wisconsin, Madison.

        McNaughton Marsh, located on the Wisconsin River in north central Wis-
     consin, is underlain by sands of the Vilas/Crivitz series.  During the
     1974 growing season primary production of five species of emergent aquat-
     ics was determined monthly.  Net primary production was found to approxi-
     mate values reported from much more eutrophic sites elsewhere in North
     America.  Daily productivity above-ground, from the beginning of growth to
     the time of peak biomass, was as follows:  Sagittaria latifolia 4.39g/m^,
     Eleocharis palustris 4.32 g/m^, Juncus effusus 10.3 g/m2, Spar cranium
     eurycarpum 8.8 g/m2 and Scirpus validus 6.3 g/m2.  Mineral nutrient
     concentrations in McNaughton Marsh plants are comparable to those reported
     elsewhere, despite the low levels of available minerals in these sandy
     soils.  The nature of the substrate is important in nutrient uptake.
     Plants growing in McNaughton Marsh appear to "skim off" nutrients from the
     substrate as rapidly as they are received from river flow and runoff.  The
     degree to which the river nutrient load can be increased without altering
     the functioning of the marsh system must be resolved by further research.
     (AA)
534. Lion, L.W. 1980.  Cadmium, Copper, and Lead in Estuarine Salt Marsh Micro-
        layers: Accumulation, Speciation, and Transport. Ph.D. Diss. Stanford,
        Univ., CA.  386 pp.
535. Lisiecki, J.B. , and C.D. McNabb.  1975.  Dynamics of Hazardous Elements  in
        Wastewater Ponds.  Project Completion Report.  Office of Water Research
        and Technology.  Washington, DC. 101 pp.  (NTIS PB-248 404/GST)

        A detailed record was developed of temperature, light, dissolved oxy-
     gen, Eh, pH, and sediment characteristics for a municipal waste  stabiliza-
     tion pond in Michigan in which the dynamics of Cu and Cd were studied over
     the growing season.  The submersed vascular plants, Ceratpphyllum
     demersum, and Potomogetqn foliosus, and the epineustic Lemma minor formed
     the principal plant population in the pond.  Their degree of participation
     in the budgets of the metals is described.  Budgets for total Cu and total
     Cd were developed for the pond at two or three week intervals from May
     through September.  For the period of study, the plants had a net loss
     equivalent to 2% of the Cu influent to the pond.  There was a net removal
     of Cd by the pond for intervals from the start of the study to mid-August.

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     The aquatic plant community  showed  a  net  gain  in Cd  until mid-August,  and
     a net loss with senescence thereafter.  The zooplankton and benthic macro-
     invertebrates of the pond showed  net  gains and losses that were  a  very
     small fraction of the total  budgets of the elements.  (NT)
536. Lohman, L.C.  1979.  An Overview of the Legal, Political, and Social
        Implications of Wastewater, pp. 459-474.  In; R.K. Bastian and S.C.
        Reed (eds.), Aquaculture Systems for Wastewater Treatment: Seminar Pro-
        ceedings and Engineering Assessment.  EPA-430/9-80-006. U.S. Environ-
        mental Protection Agency, Office of Water Program Operations, Construc-
        tion Grants Program.  Washington, DC.

        One of the ways to deal with the legal and political conflicts which
     could face a wastewater aquaculture proposal would be the establishment of
     a lead agency to coordinate planning, public information, and legal
     requirements.  Such an agency could address the specific concerns of in-
     volved federal and state agencies from a single information  source.
        Although the national water policy represents a call for  innovative
     methods of water use and treatment, the only real incentive, at present,
     is the lower construction and operating costs of a wastewater aquaculture
     facility.  These savings could be offset by discharge restrictions, extra
     testing requirements, or limitations on by-product use.  Until the
     incentive to innovate is clear, outweighing extra demands for educational
     and environmental safeguards, wastewater aquaculture will not receive wide
     consideration.
        Many of the social and political implications of such a project can be
     addressed through a properly designed citizen participation program.  The
     public is sometimes ahead of the professionals in willingness to innovate.
     Only one rule is unchangeable-all official presentations must be complete-
     ly honest.  (AA)
537. Loucks, O., R. Prentki, U. Watson, B. Reynolds, P. Weiler, S. Bartell, and
        A.B. D'Allessio.  1977.  Studies of the Lake Wingra Watershed: An
        Interim Report.  Rept. 78.  Center for Biotic Systems, Institute for
        Environmental Studies, Univ. of Wisconsin, Madison.
538. Lowry, D., and D. Yonika.  1979.  Feasibility of Wetland Disposal of
        Wastewater Treatment Plant Effluent in Massachusetts.  In; J.C. Suther-
        land and R.H. Kadlec (eds.), Wetland Utilization for Management of Com-
        munity Wastewater.  Abstracts of a conference held 10-12 July 1979 at
        Higgins Lake, MI.

        Investigations were carried out to consider the feasibility of utiliz-
     ing wetland ecosystems for the "polishing" of secondary wastewater treat-
     ment plant effluent in Massachusetts.  The study involved several distinct
     but complementary tasks.  Task 1 was designed to identify inland and coas-
     tal wetlands within Massachusetts which offered potential as sites for
     acceptance of secondary treated wastewater effluent.  Task 2 involved a
     further focusing of potential study areas down to two wetland areas, one

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     inland and one coastal.  Task 3 was designed to complement the evaluation
     process embodied in Tasks 1 and 2, by monitoring of an actual field situa-
     tion involving wetland disposal of secondarily treated wastewater efflu-
     ent.  The existing situation at Concord, Massachusetts was selected for
     this task.  At this site secondary treated wastewater effluent is dis-
     charged into the "Upper Pool" marsh of the U.S. Pish and Wildlife Service
     Wildlife Refuge.  Water quality improvements through the marsh were noted
     by annual reductions in BOD (67%), ammonia-N (59%), ortho-P  (49%), total P
     (47%) and nitrate-N (21%).  Distinct seasonal variations in marsh reno-
     vating capacities were also noted, generally marked by highest removal
     rates during the spring to mid-growing season, and lowest removal rates
     (or increase) during the winter.
        A recommendation is made to monitor in detail all existing situations
     in the State where treatment plant effluent is discharged into vegetated
     wetlands.  Multi-variate analysis relating wetland component conditions to
     water quality results might then provide further elucidation into the
     feasibility of the concept at specific locations.  (AA)
539. Ludwig, R.  et al.  1980.  The Use and Potential of Aquatic Species for
        Wastewater Treatment.  Appendix E.  The Use of Aquatic Systems for
        Wastewater Treatment: An Assessment.  Publication No. 65. California
        State Water Resources Control Board, Sacramento, CA.
540. Lunz, J.D.  1978a. The Uptake of Toxic Substances by Wetland Soils and
        Plants.   Paper presented at the National Symposium on Wetlands, Lake
        Buena Vista, FL.  November 7-10, 1978.
541. Lunz, J.D. 1978b. Habitat Development Field Investigations, Windmill Point
        Marsh Development Site, James River, Virginia; Appendix E; Environment-
        al Impact of Marsh Development with Dredged Material: Metals and Chlor-
        inated Hydrocarbon Compounds in Marsh Soils and Vascular Plant Tissues.
        Technical Report D-77-23.  U.S. Army Corps of Engineers Waterways Ex-
        periment Station, Vicksburg, MS.
542. Lyles, E.,  M. McCollum, J. Chirieleison, R. Finley, R. Engel-Wilson, and
        S.  Anderson.  1979.  A Computerized Ecological Impact Evaluation Method
        (CEIEM), pp. 365-368. In; G.A. Swanson  (ed.), The Mitigation Symposium:
        A National Workshop on Mitigating Losses of Fish and Wildlife Habitats.
        General  Technical Rept. RM-65.  Rocky Mountain Forest and Range Experi-
        m;lent Station,  U.S. Forest Service.  Fort Collins, CO.
543. Lynch, J.J. et al.  1947.  Effects of Aquatic Weed Infestations on the
        Fish and Wildlife of the Gulf States,  USDI Fish and Wildlife Special
        Scientific Report, 39, 1947, pp. 1-71.

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        Lynch evaluated the nature  and  extent  of  damage  to  wildlife and fisher-
     ies caused by water hyacinth and alligatorweed,  the amount  of  economic
     damage caused, and the effect  of weed  control programs on fisheries and
     wildlife.  Water hyacinth and  alligatorweed  provide a  serious  threat
     because once the species colonize  an area they  alter the  habitat,  diminish
     the value of wildlife/ and restrict access of recreationalists.  In Louisi-
     ana alone the fish and wildlife resource  affected annually  by  the
     encroachment of these species  is estimated to be worth $368,000,000.  In
     all cases, water under mats of hyacinth plants  exhibited  an oxygen deficit
     and was unsuitable for fish life.  Hyacinth  mats also  interfere  with the
     normal interchange of gases between the water surface  and atmosphere pre-
     venting the release of CC>2 and H2S.  It was  concluded  that  a concen-
     trated effort needs to be developed to control  the  further  spread  of
     exotic weed species in the south.  (AA)
544. MacGregor, K.A., M.S. Klein, J.S. Bazzolo,  and M.E.  Delaney.   1980.  Muni-
        cipal Solid Waste Disposal in Estuaries  and Coastal Marshlands.  U.S.
        Environmental Protection Agency, Municipal Environmental Research Lab.
        Cincinnati, OH   183 pp.

        This study was accomplished through:  questionnaire survey  on disposal
     practices of the coastal states and Puerto  Rico that identified the  extent
     of monitoring data  available; site visits to state agencies possessing
     significant monitoring data; and a survey/analysis of State and Federal
     regulations.  In addition, an extensive  search of both the formal  scien-
     tific literature and the "gray" technical report literature was conducted.
     Recommendations for improvements in regulations, for the  mitigation  of ad-
     verse environmental effects of MSW disposal in the coastal zone and  an
     extensive bibliography are also provided.   This study reached the  follow-
     ing conclusions:

        0  MSW disposal  sites should be located  to prevent leachate migration/-
           operated to minimize leachate production; and  monitored and  regu-
           lated to control the detrimental effects to the environment.

        0  The impact of municipal solid waste disposal on coastal ecosystems
           has not been analyzed in depth by  eilther the  scientific or  the
           regulatory community.

        0  The only well-documented impact is habitat depletion.   There  is a
           high probability that this is also the most serious impact result-
           ing from MSW disposal.

        0  There are circumstances in which coastal MSW disposal can be used
           for beneficial purposes, e.g., to provide a substrate for habitat
           creation.  (AA)
545. Maguire, L.A.  1974.  A Model of Beaver Population and Feeding Dynamics  in
        a Peatland at Houghton Lake, Michigan.  Master's Thesis.  Univ. of
        Michigan, Ann Arbor.

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546. Majori, L., and P. Petronio.  1973.  Marine Pollution by Metals and Their
        Accumulation by Biological Indicators (accumulation factor).  Rev.
        Intern. Oceanorg. Med. Tomes XXXI-XXXII.

        Mention is first made of the present day toxicological interest taken
     in the growing environmental pollution by potentially toxic materials.
     Especially in the marine environment, these dissolved pollutants set
     serious hygienic problems when the close contact and their affinity, as
     regards marine organisms, are considered.
        The experimental study of the effects of metal pollution simulated  in
     the laboratory, on sensitive biological subjects such as mussels, is
     useful not only in estimating the negative hygienic consequences on a
     wider scale, but also for example for purposes of pollution diagnosis  by
     using the mussel as pollution indicator.  This investigation can be aided
     by working out a suitable mathematical model of correlation between pollu-
     tion and accumulation, for descriptive and predictive purposes.
        As regards the experimental procedure, the description of the analyti-
     cal methods used in our institute for the measurement of the metals in
     marine water and in biological tissue are described, as well as the
     methods of maintenance and simulated pollution of the mussels by heavy
     metals, added as soluble ions.
        The results, obtained by using Cd++, CU"*"1", Pb++ as soluble pollu-
     tants, confirm their accumulation by the mussel and specify the hygienic
     dangers for the mussel and man, as well as the characteristics of the
     mussel as indicator of marine pollution.  The simplified model of corre-
     lation between pollution and accumulation which we use, is very useful to
     quantify these important aspects. (AA)
547. Mann, R., and R.E. Taylor, Jr.  1981.  Growth of the Bay Scallop, Argq-
        pecten irradians, in a Waste Recycling Aquaculture System. Aquaculture
        24(1/2):45-52.

        This report presents results of studies of the growth of Argppecten
     irradians both in a pilot scale waste recycling aquaculture system,  and  in
     laboratory systems at four temperatures when fed on phytoplankton cultured
     in sewage-seawater mixtures.  Juvenile A. irradians were grown  success-
     fully on a diet of phytoplankton cultured in sewage-seawater mixtures.   A.
     irradians increased from initial live and dry meat weights of 1.15 g and
     0.043 g respectively, to terminal values of 9.08 g and 0.599 g,  respec-
     tively.  This corresponds to instantaneous growth rates for live weight
     and dry meat weight of 0.009 and 0.013, respectively. High mortalities
     were evident towards the end of the experiment.
548. Mann, R., J.M. Vaughn, E.F. Landry, and R.E. Taylor, Jr.   1979.  Uptake of
        Heavy Metals, Organic Trace Contaminants and Viruses by  the Japanese
        Oyster, Crassostrea gi gas, Grown in a Waste Recycling Aquaculture  Sys-
        tem.  Tech. Rept. Woods Hole Oceanographic Institution.  Wood  Holes, MA.

        A 24 week study was carried out in which oysters  (C. gigas) were  grown
     in four regimes: on phytoplankton cultured in a mixture of  secondary
     treated sewage effluent and seawater for a period  of 12 weeks followed by

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     a second 12 week period of feeding on phytoplankton cultured in a "clean,"
     organically enriched regime; as above except that the secondary effluent
     was sand-filtered prior to use; as above except that the effluent was
     charcoal-filtered prior to use; and using "clean," inorganically enriched
     phytoplankton food for the 24 week period.  At 2 week intervals, oyster
     populations were removed for assay for trace metals (e.g., Cd, Cr, Cu, Hg,
     Ni, Pb, and Zn) and organic contaminants (e.g., hydrocarbons and PCBs). No
     significant accumulation or depuration of any metal or organic contaminant
     was evident in any of the regimes.  In terms of these contaminants all
     oysters were within acceptable edible standards as set by FDA.  A series
     of experiments carried out to examine the public health implications of
     enterovirus survival in a mollusk culture system fertilized with secondary
     treated sewage effluent used MS-2 bacteriophage and vaccine strain polio-
     virus;  apparently depuration could be effected in 20-25 days in C. gigas
     at 15 degrees C.  However, this does not mean that such a time span would
     be adequate for other enteroviruses.  (PA)
549. Mann, R., and J.H. Ryther.  1977.  Growth of Six Species of Bivalve
        Molluscs in a Waste Recycling-Aquaculture System.  Aquaculture 11(3):
        231-246.

        Growth of six species of bivalve mollusk (Grassestrea giges, Tapes
     japgnica, Mercenaria mercenaria, C. virginica, mytilus edulis, and ostrea
     edulis) was compared in a waste recycling-aquaculture system.  Tests were
     conducted at temperatures of 15 and 20 degrees C from November 1975 - May
     1976.  The animals were fed algae, mostly Phaeodactylum tricornutum, en-
     riched with secondary treated sewage effluent.  C. gigas, T. japonica, and
     2* Edulis grew well, but M. mercenaria, C. virginica, and M. edulis exhib-
     ited poor growth.  The growth of C. gigas, T. j aponica, and 0. edulis in-
     dicates the biological feasibility of producing animal protein through a
     combined tertiary sewage-treatment-aquaculture system.  Although the
     growth rates presented were not exceptional when compared with those
     recorded in natural situations, the potential for culture at high densi-
     ties appears reasonable.  (EL)
550. Mariani, G.M. 1979.  Development of a Bioassay for the Evaluation of Open
        Water Disposal of Dredged Material. Ph.D. Univ. of Texas, Dallas. 511
        pp.
551. Markarian, R.K.,  J.E. Balon, and A.C. Robinson. 1977.  A Review of
        Current Interest and Research in Water Hyacinth-Based Wastewater Treat-
        ment. Battelle Columbus Labs, Ohio. NTIS No. N77-33683. February, 53 p.

        A survey of researchers, literature, and potential users indicates that
     water hyacinth (Eichhornia crassipes) for wastewater treatment  can provide
     a lower-cost method for small cities and towns to meet increasingly strin-
     gent effluent requirements.  While there have been a few demonstrations of
     hyacinth systems during the past two years, little design data  has been
     made available.  Several full-scale projects are planned for  1977-78.  At

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     present there is no source from which to purchase a treatment system or
     design which has been approved by appropriate regulatory agencies or for
     which there are demonstrated performance capabilities or cost information.
     Such a product may be available in three or four years, however, as sever-
     al suppliers of conventional systems are testing hyacinth designs.  Con-
     trolled experiments under realistic nutrient loading conditions are needed
     to develop a satisfactory system.  Required data include: (1) optimal
     physical design (lagoon depth, area, holding time, and harvesting method-
     ology); (2) construction and operating costs; (3) plant-escape prevention
     techniques; and (4) system performance under various loading conditions
     and temperature cycles.  The best institutional arrangement for such test-
     ing would involve a joint effort of an engineering firm, wastewater treat-
     ment authority, regulatory agencies and a funding source such as EPA. The
     use, if any, of harvested plants is not of general concern.
552. Marshall, D.E.  1970.  Characteristics of Spartina Marsh Receiving Treated
        Municipal Sewage Wastes.  In; H.T. Odum and A.F. Chestnut  (eds.),
        Studies in Marine Estuarine Ecosystems Developing with Treated Sewage
        Wastes.  Inst. Marine Sci., University of North Carolina.  Annual
        Report 1969-1970.

        This study compared an effluent-fertilized and a natural salt marsh
     through an annual cycle.  On the basis of general appearance, the waste
     receiving marsh in Calico Creek produced a higher biomass of  Spartina
     alternifplia than the natural marsh, thus indicating a beneficial effect
     of fertilization. The augmented growth of Spartina and its rapid decay
     were paralleled by a high population of snails, particularly  Ljttorina
     irrorata.  The increased activity of bacteria and rate of decomposition of
     organic tissues is attributed to the release of nutrients by  dead Spartina
     grass and melampus snails.  No significant difference was detected  in the
     subsurface salinity of the two marshes.
553. Marshall, P.E., and N. Pattullo,  1981.  Mycorrhizal Occurrence  in Willows
        in a Northern Freshwater Wetland.  Plant  and Soil  59(3):465-471.

        The intensity of mycorrhizal infection  in wetland  willows  growing in
     central Michigan was not affected by  added phosphorus.  Two sites were
     compared, one water-saturated  and one drier.   P was added at  two levels,
     20 or 200 kg P per ha, to  simulate potential inputs from  the  disposal of
     secondary municipal wastewater effluent.   Scoring was based on  a 0 to 4
     scale, 2 and 3 designating the presence of a definite faunal  mantle ob-
     served by electron microscopy, with 3 having considerable intercellular
     hyphae and slight epidermal cell  elongation.   In  the  wet  site,  mycorrhizal
     infection was rated between 1.86  and  3.30, reaching a minimum in August, a
     a dry month, and a maximum in  September.   No statistical  differences were
     seen between control,  high P,  and low P,  except in August when  the infec-
     tion was greater than  control  in  the  sites receiving  P.   In the drier
     site, infection was rated  between 1.25  and 3.30,  reaching a minimum in
     August at all sites and a  maximum in  September at the control site, and a
     maximum in July in the low and high  sites.  Low and high  P sites had more
     infection than the controls in July  and August, and less  in September.

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554. Maryland Water Resources Research Center.   1974.  Program Activities.
        Water Resources Research Center. Univ. of Maryland.  (NTIS  Rept.  No.  PB-
        239686).

        Research and other program activities under way  at the Maryland  Water
     Resources Research Center during FY74 are described, and  current  and anti-
     cipated water problems are identified.  The Center's largest  project per-
     tains to the potential impact on soils, crops, and  other  vegetation of
     emissions of a large, natural draft cooling tower that  used brackish
     water.  Other problems being studied include treatment  and disposal of
     farm wastes, urban storm runoff, chlorinated sewage effluents,  heavy
     metals and minerals in Chesapeake Bay sediments, water  pollution  effects
     on aquatic life, and contributions of tidal marshlands  to food  chains in
     estuaries.  (EL)
555. Massachusetts Inst. of Tech.   1977.  Electron Irradiation,  Sewage  Sludge,
        and Aquaculture.  MIT/Marine Industry Collegium Opportunity Brief No.
        6.  Sea Grant Rept. No. 77-14.  Massachusetts Inst. of Tech.  Cambridge,
        MA.  31 pp.

        The current state of electron irradiation as a sludge sterilization
     method is reviewed.  The costs with existing methods are compared  and some
     economic projections about the production and sale of sludge  as  fertilizer
     are made.  Given that the irradiation treatment of sewage sludge produces
     a nutrient-rich, sterile medium for the culture of microorganisms, a con-
     trolled ecosystem could be designed using this nutrient energy base upon
     which to structure a food web  capable of supporting the commercial pro-
     duction of marketable human or animal foods.  Such a contrived ecosystem
     might be managed intensively to achieve high efficiency of  production of
     desired high-value aquatic species.  Alternatively, extensive culture
     systems based on irradiated sludge might be managed to produce large quan-
     tities of animal feed composed essentially of algal or fungal single-cell
     proteins. (NT)
556. Mattson, C.P, et al.  1975.  Nitrogen Import and Uptake Measured on
        Marshes of the Hackensack River in New Jersey.  Paper Presented at  38th
        Ann. Meeting Amer. Soc. Limnol. Oceanogr., Halifax, Nova Scotia.  1975.
557. Mayes, R.A., Mclntosh, and V.L. Anderson.  1977.  Uptake of Cadmium and
        Lead by a Rooted Aquatic Macrophyte  (Elodea canadensis).  Ecol. 58:
        1176-1180.

        The role of roots in the uptake of nonessential trace metals by aquatic
     macrophytes was investigated using Elodea canadensis.  Plants were grown
     in 2 lakes in which metal concentrations in the water differed.  Specimens
     in each lake were anchored in either control sediment or in sediment con-
     taminated with Cd and Pb.
        Plants grown in the same water but in sediment from different sources
     had significantly different concentrations of the 2 metals.  Elodea sam-

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     pies rooted in sediments from the same source but grown in water with
     different levels of metals also accumulated significantly different
     amounts of Cd and Pb.   Thus the importance of both sediment and the sur-
     rounding water as sources of metals for aquatic plants was demonstrated.
     Samples grown in water and sediment containing low levels of metals had
     minimal concentrations of 0.32 microgram Cd/g and 5.2 microgram Pb/g,
     while plants grown in metal-contaminated water and sediment accumulated up
     to 32.33 ug Cd/g and 1609 microgram Pb/g.  (AA)
558. Maystrenko, Yu. G., A.I. Densiova, V.M. Bagnyuk, and Zh. M. Aryamova.
        1969.  The Role of Higher Aquatic Plants in the Accumulation of Organ-
        ic and Biogenic Substances in Water Bodies.  Hydrobiological Journal
        5(6):10-31.
559. McCaffrey, R.J.  1977.  A Record of the Accumulation of Sediment and Trace
        Metals in a Connecticut Salt Marsh.  New Haven Ct. Yale University,
        Ph.D. Dissertation, 156 p.
560. McCalley, D.V., M. Cooke, and G. Nickless.  1980.  Coprostanol in Severn
        Estuary Sediments.  B. Env. Contam. and Tox. 25(3):374-382.

        Occurrence of the fecal pollutant coprostanol in the Severn, U.K.,
     estuary was measured. The Severn estuary, which receives sewage outfalls,
     is used as a public bathing area. Degradation rates of coprostanol in
     estuarine environments is unknown.  However, coprostanol concentrations in
     Severn water averaged only 0.1-0.3 ppb.   (EL)
561. McCool, M.M.  1921.  Peat and Muck Soils, Fixation of Fertilizers. Mich.
        Quart. Bull. 3:126-127.
562. McCormick, J. 1970.  The Natural Features of Tinicum Marsh, with Particu-
        lar Emphasis on the Vegetation, pp.  1-104.  In: J. McCormick, R.R.
        Grant, Jr., R. Patric.  Two studies  of Tinicum Marsh, Delaware and
        Philadelphia Counties, PA.  The Conservation Foundation, Washington.
        123 pp.
563. McCormick, J., R.R. Grant,  and R. Patrick (eds.).   1970.  Two Studies  of
        Tinicum Marsh, Delaware  and Philadelphia Counties, PA.  The Conserva-
        tion Foundation.  Washington, DC.

        Extensive  study on  the natural features and purification  capability of
     Tinicum Marsh in Pennsylvania, illustrated that  "marshlands  play  an  im-
     portant role  in reducing the  nutrient  load in water and  in increasing  the

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     oxygen content."  This marsh,  however,  had  "been severely injured by the
     organic pollution."
564. McDonald M.G., and W.B. Fleck.   1978.  Model Analysis  of the Impact on
        Ground-Water Conditions  of the Muskegon County Wastewater Disposal Sys-
        tem, Michigan, U.S. Department of the  Interior, Geographical Survey.
565. McDonald, R.C. and B.C. Wolverton.   1979. Comparative  Study  of  Wastewater
     Lagoon with and without Water Hyacinth.  Econ.  Bot.  34(2):101-110.

        A three year study was conducted  on an existing,  one  cell,  facultative
     sewage lagoon having a total surface area of  3.6  hectares  and  receiving a
     BOD5 loading rate of 44 kg/ha/da  (40 Ibs/ac/da).   The  comparative exper-
     imental periods ran from July through November  for three consecutive
     years.  During the first period,  water hyacinths  completely  covered the
     lagoon.  The water hyacinth coverage was reduced  to  33%  of the  total sur-
     face area the second year.  The lagoon, free  of all  vascular aquatic
     plants the third year, was•monitored for comparative purposes.   The most
     significant improvement overall in the effluent quality  occurred when
     water hyacinths covered the entire lagoon.  During this  period  the  efflu-
     ent 8005 and TSS were 23 and 6 mg/1,  respectively.   Without  water hya-
     cinths, the effluent BOD5 and TSS were 52 and 77  mg/1, respectively.
     The effluent total organic carbon concentration with water hyacinths
     averaged 40 mg/1 and without water hyacinths, 72  mg/1.   A  complete  dis-
     cussion of the results from this  three year study is presented  in this
     paper along with any associated problems that were observed  when water
     hyacinths were introduced into the lagoon and altered  its  behavior  from
     that of a normal facultative lagoon.  (AA)
566. McGarry et al.  1974.  Wastewater Reclamation Under Tropical  Conditions.
        Process.  Biochem.  9(7).

        Under tropical conditions, nitrogen can be removed  from wastewater  by
     growing algae.  Processes pertaining to wastewater pretreatment,  algal
     harvest by flotation, chlorination of the final effluent, and removal  of
     low-level organics by semi-rapid filtration are described and compared to
     adsorption by activated carbon produced from locally available waste
     products.
567. McGarry, M.G. 1977.  Domestic Wastes as an Economic Resource:   Biogas  and
        Fish Culture, pp. 347-365.  In;  Water, Wastes, and Health  in Hot Cli-
        mates.  John Wiley and Sons, New York.

        Methane produced through fermentation of farm and human wastes  is
     assessed as an alternative to increasingly expensive petroleum-based
     fuels.  This fermentation takes place in a "biogas" unit, which accepts
     waste materials that digest without access to oxygen.  The biogas,  largely

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     a mixture of methane and carbon dioxide, bubbles out of the liquid, is
     trapped, and may be used as fuel for household lighting or cooking.  The
     digested mixture or slurry can be used on the land as a soil conditioner
     and fertilizer.  Various biogas plant designs are described, and the ad-
     vantages of each are assessed.  Fish farming, also, is considered as a
     means of handling waste materials.  The wastes are introduced into fish
     ponds, where they provide the main source of nutrients for bacterial
     growth.  The byproducts from the wastes' bacterial decomposition, such as
     ammonia and CC>2/ are primary nutrients for the algae, which, in turn,
     are the basic form of food for many of the fish used in pond culture. (EL)
568. McGarry, M.G. 1979.  The jjnportance of Waste Re-use, pp. 91-116.  In; Pro-
        ceedings of the ASCE Appropriate Technology in Resource Conservation
        and Recovery Symposium.  Held Oct. 22-26 in Atlanta, GA.

        Human wastes are regarded as a valuable resource in China.  Their fer-
     tilizer components are valued in agriculture and fish farming operations.
     Current practices employed in China for the reuse of sewage are surveyed.
     Composting systems include granular composting, thermophilic composting,
     and composting toilets.  Other uses for this waste, such as direct algal
     harvesting or gas generation, are being researched in developing nations.
     (EL)
569. McHarg, I.L.  1976.  Biological Alternatives to Water Pollution, pp. 7-13.
        In; j. Tourbier and R.W. Pierson (eds.), Biological Control of Water
        Pollution.  Univ. of Penn. Press.
570. Mclntosh, A.W., E.K. Sheppard, R.A. Mayes, G.J. Atchison, and D.W. Kelson.
        1978.  Some Aspects of Sediment Distribution and Macrophyte Cycling of
        Heavy Metals in a Contaminated Lake.  J. Environ. Qual. 7:301-305.

        Distribution and forms of the metals cadmium (Cd) and zinc  (Zn) in
     sediment and the possible significance of a die-off of the aquatic macro-
     phyte Pptamggetpn crispus in Cd cycling in a metal-contaminated  lake were
     studied.  Metal levels in the upper 5 cm of lake sediment ranged from  2.54
     ppm Cd and 115 ppm Zn in an uncontaminated area of the lake to 805 ppm Cd
     and 6,120 ppm Zn near the entrance of a metal-bearing ditch to the lake.
     Evidence of metal contamination existed at a depth of 30 cm in sediments
     in contaminated areas of the system.  Dominant forms present in  the sedi-
     ment were the carbonate for Cd and carbonate and organic for Zn. Analysis
     indicated Cd levels as high as 89.6 ppm in P. crispus in the lake with a
     maximum burden of 1.5 kg Cd held by the lake's P. crispus population.
     Release of the total amount could raise water concentrations by  a maxi-
     mum of 1 microgram/liter.  (AA)
571. McKim, H.L.  (Sym. Coor.)  1978.  State  of Knowledge  in Land Treatment of
        Wastewater.  Vol.  1 and Vol. 2.  International  Symposium 20-25.  August

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         1978.  U.S. Army Corps  of Engineers.   Cold  Regions  Research and
        Engineering Lab.  Hanover, NH.

        The objectives of this  Symposium are  to  summarize the  state of
     knowledge of the physical  aspects  of the treatment  of  wastewater  by land
     application and to identify the  suitable approaches for the  design of  such
     land treatment systems.  The topics to be included  are: site selection
     considerations, case studies of  national and international concern, health
     effects of land treatment  systems,  pretreatment  considerations, uses of
     wastewaters in agricultural and  forest systems,  monitoring,  modeling and
     design criteria.
        The Symposium Proceedings are published  in  two volumes.   Volume 1 con-
     tains the invited papers presented and discussed at the conference. Volume
     2 contains shorter papers  about  on-going research that were  selected from
     the responses received following a call  for abstracts.
572. McKinney, R.E. 1976.  Functional Characteristics Unique  to Ponds.   In;
        E.F. Gloyna, J.F. Malina, Jr., and E.H. Davis  (eds.), Ponds  as  a Waste-
        water Treatment Alternative.  Water Resources Symp. #9. Center  for
        Water Research, College of Engineering, Univ. of Texas, Austin.
573. McLarney, W., and M. Sherman.   1974. Midge Culture  at New Alchemy  East.
        Aquaculture and the Pish Farmer  1:10-13.
574. McLeod, K.W., and C. Sherrod, Jr.   1980.  Revegetation  of Thermally
        Altered Swamp Forest.  Association of Southeastern Biologists  Bull.
        27(2):49-50.

        For 15 years, thermal effluents  were introduced  into Stell Creek,
     located on the Savannah River Plant (Georgia).  Drastic alteration  of the
     forest occurred during this period  with complete  loss of the canopy trees.
     Succession following cessation of thermal effluents in  this totally open
     habitat has been slow and developed more similar  to an  isolated semiaquat-
     ic habitat, rather than a continuous swamp forest.  It  is important to
     determine if the establishment requirements for the formerly dominant
     species are met by the "new" Steel  Creek habitat.  Studies investigating
     the germination and seedling growth of Nyssa aquatica,  Salix nigra, and
     Taxodium distichum are discussed.   Apparently dispersal mechanisms  are
     responsible for creating a biotic environment which has restricted  the
     establishment of the formerly dominant species, water tupelo and  bald
     cypress.  (PA)
575. McMahan, E.A.  1972.  Relative Abundance of Three Marsh Floor Organisms  in
        a Sewage Affected Marsh and in a Sewage-free Marsh.  J. Elisha Mitchell
        Sci. Soc. 88(2):61-65

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        Three marsh-floor dwellers were considered as possible biological indi-
     cators of pollution in marine habitats after sampling a spartina marsh
     exposed to treated sewage wastes and a similar but nonpolluted marsh. Sys-
     tematic sampling of the marsh surface and the upper 2 cm of substrate was
     carried out in both marshes to compare population densities of the
     amphipod, Talorjshestia Ignjgricornis, the collembolan Sminthurides rubron-
     jLveus var. leyanderi and the tubificid Monopylephgrus rubroniveus.  The
     amphipods and collembolans were sampled with a shop vacuum cleaner with an
     extension tube plugged into a portable generator.  Worm samples were taken
     by pusing a plastic jar, open and down, into the mud to about 2 cm depth.
     A small plastic spade was used to undercut the jar, trapping the substrate
     inside.  Significantly more collembolans were captured in the sewage-free
     marsh, and significantly more amphipods and tubificids were captured in
     the polluted marsh.  Presence of amphipods, tubificids, and collembolans
     in both polluted and sewage-free marshes complicates their use as
     biological indicators.  More studies, involving relative densities of all
     substrate inhabitants seem indicated.  Some other factor of factors—such
     as sediment size, Spartj.na density, current patterns, or predator
     pressure—are also considered.
576. McMahan, E.A., and L. Davis.  1978.  Density and Diversity of Microarthro-
        pods in Wastewater Treated and Untreated Cypress Domes, pp. 429-462.
        In; H.T. Odum and K.C. Ewel  (eds.), Cypress Wetlands for Water Manage-
        ment, Recycling and Conservation.  Fourth Annual Report. Univ. of Flor-
        ida, Gainesville.
577. McMahan, E.A., R.L. Knight, and A.R. Camp.   1972.  A Comparison  of Micro-
        arthropod Populations in Sewage-exposed and Sewage-free Spartina  Salt
        Marshes.  Environmental Entomology 1:244-252.

        An investigation of effects of sewage effluents on populations of
     microarthropods living in Spartina. marshes was carried out in  the spring
     and summer of 1970.  Using primarily a. vacuum collecting technique,  com-
     parisons of dominant forms, population densities, and species  diversities
     were made between populations inhabiting marsh grass exposed to  flooding
     by treated sewage wastes and those in nonpolluted areas.  Only in the case
     of spiders and amphipods were significant differences found, with density
     in both cases being greater in the polluted  marsh.  Species diversity wasd
     relatively high, especially for populations  from Sjpartina growing within
     margins of artificial ponds, some of which were  also exposed to  treated
     sewage wastes.
578. McNabb, C.D. 1976.  The potential of  Submersed Vascular Plants  for Recla-
        mation of Wastewater in Temperate  Zone Ponds.   In;  J.  Tourbier  and R.W.
        Pearson  (eds.), Biological Control of Water Pollution.   The  University
        Press, Philadelphia, PA.

        The productivity of submersed vascular plants  in a series  of wastewater
     treatment ponds was assessed.   In the temperate climate of  Michigan,

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     submersed plants demonstrated a doubling time of 11 days during  the  summer
     (>10°C) until light became a limiting factor due to dense algal  blooms.
     The submersed vascular species flourished best in an environment where
     Daphnia magna and D. pulex decreased algal densities through  grazing and
     thus increased light penetration and clarity. Nutrients were  accumulated
     primarily from the water through foliage rather than from the  sediments
     through the roots.  Strategies for harvesting submersed aquatic  macro-
     phytes for maximum yield are discussed.  (AA)
579. McNabb, C.D., Jr., and D.P. Tierney, 1972.  Growth and Mineral
        Accumulation of Submersed Vascular Hydrophytes in Pleioeutrophic
        Environs, Department  of Fisheries and Wildlife, Michigan State
        University, Technical Report No. 26.

        Submersed vascular hydrophytes invade ponds designed  for stabilizing
     municipal waste.  The adaptability of several species for vegetative
     growth in pleioeutrophic environments was compared by measuring  growth
     rates.  Coontail has a range of tolerance that includes  the extremes  in
     water quality that are observed in wastewater ponds.  The tissue concen-
     tration of phosphorus in submersed plants tended to increase linearly with
     concentrations of soluble phosphorus in the ambient water.  A similar re-
     lationship regarding nitrogen was not found.  Heavy metals were  not dis-
     cussed or studied.  (AA)
580. McNabb, C.D. Jr., D.P. Tierney, and S.R. Kosek, 1972.  The Uptake  of Phos-
        phorus by Ceratophyllum demersum Growing in Wastewater.  Michigan State
        Univ., Department of Fisheries and Wildlife, East Lansing.  Institute  of
        Water Research Technical Rpt. No. 24.  23p.

        The cultivation and harvest of aquatic plants at wastewater treatment
     sites has long been suggested as a first step in recycling minerals. Po-
     tential of an aquatic angiosperm for sequestering harvestable  phosphorus
     during growth in a system of sewage oxidation ponds is evaluated.  Experi-
     mental plants of geratojphyllum demersum, a cosmopolitan aquatic angio-
     sperm, were selected from a naturally occurring clone in a .pond on the
     Michigan State University campus.  Actively growing tips, 30 cm in length,
     were transplanted to pots placed on the bottom of the third lagoon in a
     series receiving raw waste from a city.  The plant population  approached
     its stationary phase of growth in 57 days.  Its dry weight and growth rate
     compared favorably with maximum rates reported for other aquatic
     macrophytes.  Following transplant, phosphorus concentration in the
     tissues doubled.  Reflecting environmental differences in the  amount of
     the element available to the plants, the terminal phosphorus concentration
     in tissue in 1.3% of dry weight is twice the maximum value ordinarily ob-
     served in field studies.  Results suggest that continued attention be
     given to development of managed crops of aquatic macrophytes as one compo-
     nent is mineral recycling schemes using wastewater.

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581. McNaughton, C.D.  1974.  Heavy Metal Tolerance of Typha Ij^tifolia without
        Evolution of Tolerant Races.  Ecology 55:1163-1165.

        Clones of the broad-leaved cattail (Tyjpha latifplia L.) and soil sam-
     ples were obtained from near a zinc smelter and from a control location.
     In the smelter location/ soil zinc concentration was 385 times higher,
     cadmium content 37 times higher, and lead content 16 times higher.  No
     evidence for the evolution of heavy metal tolerance could be detected in 2
     X 2 experiments in which genotypes from both locations were grown on both
     soils.  Growth of genotypes from both locations was inhibited on the heavy
     metal soil, but not to the extent that would be expected from previous
     studies of heavy metal effects.  This is the first case described in which
     a species has been able to colonize heavy metal soils in the absence of
     the evolution of tolerant races.  (AA)
582. McVea, C., and C.E. Boyd.  1975.  Effects of Water Hyacinth Cover on Water
        Chemistry, Phytoplankton, and Fish in Ponds.  J. of Environ. Qual.
        4(3):375-378.

        Water hyacinth  [Eichhgrnia crassipes (Mort.) Solms] cover of 0, 5,  10,
     or 25% surface was established in fertilized ponds stocked with the fish,
     Tilapia aurea (steindachner), at Auburn, Alabama.  Measurements of water
     chemistry, phytoplankton density, and fish production were made during the
     1973 growing season.
        Phytoplankton production was less in ponds with 10 and 25% cover by
     water hyacinth than in ponds with 0 and 5% cover.  Competition of water
     hyacinth with phytoplankton involved shading and removal of phosphorus
     from the water.
        Concentrations of dissolved oxygen were lowest in ponds with 25% cover
     but oxygen tensions in all ponds were adequate for survival and fish
     growth.  (AA)
583. Meany, R.A. , I. Valiela, and J.M. Teal.  1976.  Growth, Abundance,  and
        Distribution of Larval Tabanids in Experimentally Fertilized Plots on
        a Massachusetts Salt Marsh.  J. Appl. Ecol. 13(2):323-332.

        On the Great Sippewissett Marsh (Tabanus nigrovittatus and Chryspps
     fuliginosus) comprised approximately 90% of the total  larvel tabanid pop-
     ulation and were both predominantly found in areas where the short  form of
     Spartina alternifIpra was the dominant plant.  The other two species are
     T. lineola and C. atlanticus.  The average larval densities for T.  nigrp-
     yittatus and C. fuliginosus found in the control plots of 1972-3  and the
     1974 plots are comparable to the maximum values for T. nigrpyittatus ob-
     tained elsewhere on the east coast of North America.   Larval tabanid pop-
     ulations were drasticallyl reduced in plots treated with a sewage sludge
     fertilizer but were unaffected by treatments with a phosphate and a urea
     fertilizer.  Analysis of monthly larval size classes for the 1972-3 sampl-
     ing period indicates that the larval life of T. nigrovittatus probably
     lasts 2 yrs.  The summer growth rate is about 4mm/mo.  T. nigrovittatus
     feeds on most soft-bodied insect larvae found on the marsh and also on  the
     amphipod Orchestia grillus.  Larval densities were reduced in laboratory

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     experiments from 80-240/m2 to 40-120/m2 through cannibalism, the  lower
     figure approximating the densities of larval tabanids found in the  field.
     (BA)
584. Mechenich, D.J.  1980.  Tertiary Wastewater Treatment Using  a Natural Peat
        Bog.  Master's Thesis, College of Natural Resources, Univ. of Wisconsin
        Stevens Point.  136 pp.

        Significant reductions in effluent concentrations of chloride  (60%),
     alkalinity (100%), hardness (83%), phosphorus  (80%), and total  suspended
     solids (95%), were noted based on differences  between the effluent  and bog
     outflow water quality during the effluent discharge period.  Effluent pH
     and conductivity were also significantly reduced, while COD  increased 204%.
        Chloride tracing revealed that the effluent water primarily  moved
     through the bog in the upper 0.5 meter. Other  than chloride  and conductiv-
     ity increases, the most significant water quality changes within the bog
     appeared to be a drop in COD, BOD, phosphorus, and nitrogen  below the es-
     tablished background levels at several well sites, and a reduction  in
     background COD in the bog discharge.
        Leaching studies using Drummond peat cores  were used to estimate the
     long-term potential of the bog to remove nutrients by physical-chemical
     processes.  Under the specific experimental conditions, net  phosphorus
     removals up to 0.19 mg P/gram oven dry weight  peat were found,  which was
     equivalent to approximately 40 kg P/acre-foot  of surface peat soil.
     Nitrogen and calcium removals of 0.45 and 4.5  mg/gram oven dry  weight peat
     respectively were also found.  (AA)
585. Meeks, R.L. 1969.  The Effect of Drawdown Date on Wetland Plant Succes-
        sion.  J. Wildl. Manage.  33(4):817-821.

        A 7-year study was begun on the Winous Point Shooting club in  1956 to
     determine the effect of drawdown date on plant succession.  An 80-acre
     marsh was diked into four units, one of which was drained yearly  in mid-
     March, one in mid-April, one in mid-May, and one in mid-June.  All the
     units were reflooded during September. Plant succession followed  the same
     general trend on all units, going  from semi-aquatic species to predomi-
     nantly annual weeds. Fewer years were required within early drawdown for
     annual weeds to replace semi-aquatic species.  The May drawdown unit had
     the best plant associations for wildlife after 7 seasons.  Draining dur-
     ing mid-to-late May should allow muskrats (Ondatra zibethiga) to  raise two
     litters without interruption, and not interfere with duck nesting.  (AA)
586. Mees, Q.M., and J.R. Hensley.  1974.  Survival of Pathogens in Sewage Sta-
        bilization Ponds.  Final Rept. Dept. of Civil Engineering, Univ. of
        Arizona, Tucson.  114 pp.

        In semi-arid areas where water supplies are being depleted at alarming
     rates, re-use of water is of paramount importance.  The investigation was
     initiated in an attempt to determine the survivability of amoebic cysts,

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     intestinal flagellates, and ova of helminths at various phases of the  sta-
     bilization process.  Only those organisms pathogenic to man were to be
     investigated.  (NT)
587. Meo, M., J.W. Day, Jr., and T.B. Ford.  1979.  Land Treatment of Fish
        Processing Wastes on Dredge Spoil Sites:  Comparative Cost Evaluations.
        Coastal Zone Management Journal 3(3):307-318.
588. Merezhko, A.I.  1973.  Role of Higher Aquatic Plants in the Self-purifica-
        tion of Lakes and Streams.  Hydrobiol. J. 9:103-109.
589. Merritt, R.W. 1978.  Insect Pest Problems Associated with Wastewater Oxi-
        dation Lakes in Michigan.  Project Completion Rept. to Office of Water
        Research and Technology.  Dept. of Entomology.  Michigan State Univ.
        East Lansing, MI.  47 pp.

        The distribution and abundance of larval Chironomidae were investigated
     in the first and last lakes of a 4 lake sewage oxidation system.  Chiromid
     species composition and adult seasonal occurrence were examined and the
     potential of species to become pests of man was evaluated.  A stratified
     random sampling program was employed to quantitatively sample larvae in
     Lakes 1 and 4.  Each lake was stratified by depth, distance from the
     influent site, and wind.  Samples were taken during the summer and early
     fall of 1976 and during late spring and early summer 1977.  Chironomidae
     was the dominant subfamily in both lakes initially; the grthocladiinae
     gradually becoming dominant in Lake 4 by early summer 1977.  The patterns
     of larval distribution and abundance in Lakes 1 and 4 indicate that the
     Chironidae actively responded to differences in oxygen and nutrient avail-
     ability in the sediments by migrating to areas offering favorable condi-
     tions.  Oxygen appeared to be the limiting factor in Lake 1, nitrogen and
     phosphorus in Lake 4.  (NT)
590. Merrell, J.C., Jr., W.F. Jopling, R.F. Bott, A. Katko, and H.E. Pintler.
        1967.  Santee Recreation Project, Santee, California. Final Rept. to
        Federal Water Pollution Control Administration, Cincinnati, OH.  174 pp.

        The report presents the results of a study of the Santee, California
     recreational lakes, which were deliberately planned to utilize the  commu-
     nity 's reclaimed sewage effluent.  By the summer of 1965, a special basin
     adjacent to one of the lakes was supplied with reclaimed water and  used
     for swimming.  This was found possible after progressive use of the lakes
     for boating and then for fishing. No health hazards have been demonstrated
     by the viral or other findings.  (NT)
591. Metcalf, T.G. 1975.  Human Enteric Viruses in a Waste Recycling Aquacul-
        ture System.  NTIS No. PB 245 909 Final Report. Nat. Sci. Found. 32 pp.

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     Potential public health hazards  from the use  of  the  aquaculture waste re-
     cycling system were evaluated.   The  treatment system consisted of algal
     ponds  in which secondary  non-chlorinated sewage  was  mixed with seawater,
     and raceways stocked with shellfish.   Test  viruses were Poliovirus II,
     Echovirus 5, Coxackievirus B-3,  Reovirus 1, and  type 5  Adenovirus. Results
     of the two-year study indicated  that the system  is unacceptable,  if a
     virus-free effluent is desired,  without  a second virus  inactivating pro-
     ceses.  If virus-free shellfish  were to  be  the parameter of  public health
     acceptance, the full-grown shellfish could  be depurated to remove viruses
     or the sewage effluent could be  treated  to  remove viruses.  The system was
     judged unsafe for  use as  a means of  providing a  growth  medium for commer-
     cially valuable marine life.  Further research was recommended.
592. Mhatre, G.N., S.B. Chaphekar,  I.V.  Ramani  Rao,  M.R.  Patil,  and B.C.
        Haldar.  1980.  Effect of Industrial  Pollution  on the Kalu River  Eco-
        system.  Environmental Pollution;  Series  A:  Ecological and Biological
        23(1):67-78.

        Vegetation stands above and below  industrial discharge points in  the
     Kalu River  (India) were studied for relative frequency,  relative density,
     and relative dominance of the  component  species.   Samples of  water,  sedi-
     ment, vegetation, and industrial effluents were examined for  Cu, Cd, Pb,
     and Hg by AAS.  Aquatic life was severely  affected at Ambivali due to
     toxic discharges from local mills.  Although the water quality was not
     satisfactory at either Ambivali or  Titwala,  it  was much  worse at Ambivali.
     Sediments along the intertidal banks  of  the  river  at both locations  showed
     high concentrations of metals.  The diversity of plants  in  the intertidal
     region at Ambivali was low compared to that  at  Titwali;  10  species were
     found at Ambivali, while 20 were present at  Titwali.  The plant community
     along the riverbank at Ambivali was dominated by only two species, Cynodon
     da.ctyj.qn and Pycreus macrostachyos, which  showed high Importance Value
     Indexes.  However, they showed no similar  dominance  values  at Titwali with
     higher floristic diversity.  Replacement of  a diverse vegetation by  a few
     tolerant species with a tendency to accumulate  toxic  metals opens up an
     interesting field of dynamics  of plant populations facing severe pollution
     stress.  (PA)
593. Mickle, A.M., and R.G. Wetzel.  1979.  Effectiveness of Submersed Angio-
        sperm-epiphyte Complexes on Exchange of Nutrients and Organic Carbon in
        Littoral Systems.  III.  Refractory Organic Carbon. Aquatic Botany
        6(4):339-355.
594. Middlebrooks, E.J. 1980.  Aquatic Plant Processes Assessment, pp. 43-62.
        In; S.C. Reed and R.K. Bastian (eds.), Aquaculture Systems for Waste-
        water Treatment: An Engineering Assessment.  EPA-430/9-80-007. U.S.
        Environmental Protection Agency, Office of Water Program Operations,
        Municipal Construction Division. Washington, D.C.

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        The most common water hyacinth wastewater treatment system incorporates
     a stabilization pond followed by series-type water hyacinth culturing
     tanks.  The design characteristics of hyacinth systems are discussed in
     this report, and this paper's conclusions are that:

        0 Treatment process appears to be applicable in warm temperate and
          tropical climates, and adequate data appears to be available to
          assist in the design of a system capable of producing an advanced
          secondary effluent.

        0 Water hyacinths thrive in municipal wastewaters and appear to do
          well in mixtures of municipal and industrial wastewaters.

        0 A hydraulic loading rate of 2,000 m4/ha/day to a hyacinth system
          appears reasonable when treating secondary wastewater treatment plant
          effluent if nutrient control is not an objective.
595. Mihursky, J.A. , 1969.  On Using Industrial and Domestic Wastes in Aqua-
        culture.  Agricultural Engineering. 50( 11):667-689.

        Three waste materials have the greatest potential for recycling through
     aquatic systems into useful nutrients—petroleum wastes, sewage wastes,
     and waste heat.  Protein developed and sold in France is produced by
     microorganisms growing on waste low-grade petroleum.  Large-scale use of
     ndcrobiologically derived proteins seems possible. Sewage converted to in-
     organic materials may provide fertilizer for algal production.  Waste heat
     may assist in providing optimal environmental conditions for biological
     growth at all trophic levels.  In various world situations sewage or sew-
     age products are used to stimulate fishery production.  Algae grown in
     mass aided by water from treated sewage and concentrated by evapodrying
     technique produces food for chickens.  Waste heat from  steam electric
     stations is used to raise carp in latitudes farther north than usual and
     to extend the growing season in some localities.  A waste flue gas was
     used to supply carbon dioxide gas to mass-culture an algal food supply.
     Wild or natural water is preferable to pond culture.  Running water
     systems permit dense stocking, waste removal and continued dissolved oxy-
     gen renewal.  Costs of producing fish protein concentrate through aqua-
     culture plus waste utilization will be subsidized by society's need to
     recycle its waste materials into useful foodstuffs.
596.  Miller, H.E. 1973.  Production of Fathead Minnows and Muskellunge  (North-
        ern and Tiger) in Municipal Wastewater Stabilization Systems.  Michigan
        DNR.  Fisheries Div. Grand Rapids, MI.
597. Miner, J.R., J.W. Wooten, and J.D. Dodd.   1971. Water Hyacinths to Further
        Treat Anaerobic Lagoon Effluent, pp. 170-173. In; Livestock Waste Man-
        agement and Pollution Abatement.  American Society of Agricultural En-
        gineers.  St. Joseph, MI.

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        Effluent from an anaerobic lagoon treating  liquid  swine manure  was
     pumped through a series of four pools, each ten  feet  in  diameter.  Water
     hyacinths were grown on these pools in an effort to provide  further  treat-
     ment.  The plants flourished, necessitating weekly harvesting  of one-
     fourth of the growth.  During the month of July  1970,  nine pounds  of ammo-
     nia were added to the system in the influent and less  than one-fourth pound
     discharged in the effluent.  During this same  period,  28 pounds of COD
     were added and 2.6 pounds discharged.  Extrapolating  the system to a per
     acre basis indicates ammonium nitrogen removal to be  in  excess of  35
     pounds per acre per day, COD removal to exceed 100 pounds per  acre per  day
     and phosphate removal to exceed 15 pounds per  acre per day.  Nitrate re-
     lease was less than 0.3 pounds per acre per day.  In  one sample weekly
     harvest (Aug. 14-Aug. 21), 450 plants with a total wet weight  of 90  Ibs.
     were removed from two of the ponds having a combined  area of approximately
     160 sq.ft.  This corresponds to an increase of over 17,000 new plants per
     acre per day and an increase in wet weight of  over 2500  pounds per acre
     per day.  At a 4% dry weight conversion factor,  this  equals  100 pounds  of
     dry weight per acre per day.  The system has performed satisfactorily
     showing potential as a means of removing nutrients from  partially  treated
     animal wastes which are not removed by currently used  processes.
598. Mitsch, W.J. 1975a.  Observation on Utilization of Wetlands Ecosystems  for
        Sewage Treatment, pp. 90-113.  In; H.T. Odum, K.C. Ewel, J.W. Ordway,
        and M.K. Johnston (eds.), Cypress Wetlands  for Water Management,  Recy-
        cling, and Conservation.  Third Annual Report.  Center for Wetlands.
        Univ. of Florida.  Gainesville.
599. Mitsch, W.J. 1975b. Systems Analysis of Nutrient Disposal  in Cypress Wet-
        lands and Lake Ecosystems in Florida.  Ph.D. Diss., Univ. of Florida,
        Gainesville.  421 pp.

        Models, field measurements, and computer simulations were used  to eval-
     uate alternative systems of man's nutrient cycling using a freshwater  lake
     and cypress swamps in Florida.  Ecological characteristics, nutrient bud-
     gets, organic productivity, energy relations, and interfaces with  man's
     economy were compared between the two systems.  The two systems responded
     similarly to the addition of nutrients through the introduction of plants
     with low quality structure, intermediate in biomass between phytoplankton
     of the lake and trees of the dome.  Preliminary evaluation of cypress
     dome, lake and technologically based nutrient disposal alternatives sug-
     gest that the cypress system may realize a higher work service per pur-
     chased energy invested.  (AA)
600. Mitsch, W.J. 1976a. Ecological Engineering Through the Disposal of Waste-
        water into Cypress Wetlands in Florida.  In; Proceedings of the Nation-
        al Conference on Environmental Engineering Research, Development and
        Design.  Univ. of Washington, Seattle.

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601. Mitsch, W.J. 1976b.  Ecosystem Modelling of Water Hyacinth Management  in
        Lake Alice, Florida.  Ecol. Modelling,  1:68-69.
602. Mitsch, W.J.  1977.  Water Hyacinth  (Eichhornia crassipes) Nutrient Uptake
        and Metabolism in a North Central Florida Marsh.  Archiv. fur Hydrobio-
        logie. 81(2):188-210.

        Effects of high nutrient loading  from secondary  sewage effluent on  the
     marshy eastern part of Lake Alice, Gainesville, Florida were studied,  with
     special attention to the disposition of nitrogen and phosphorus/ and to
     the metabolism of water hyacinth (Eichhornia crassipes) which dominates
     the marsh.  Construction of an earthen dam in 1948  and addition of the
     sewage effluent and heated once-through condenser water from a heating
     plant in 1964 increased the lake's size from four to thirty-three ha.
     High nutrient levels and water temperatures in the  29-31 range have
     resulted in gross primary productivity among the highest ever reported—
     19.3 g C/sq m/day for large hyacinths and 15.6 g C/sq m/day for dwarf
     hyacinths.  Net production efficiencies were about  1.6% of solar insola-
     tion for both plants.  Uptake of total nitrogen averaged 49% across the
     marsh.  Nitrate showed a seasonal pattern of greater uptake from winter to
     summer; phosphorus uptake averaged 11% with signs of a net phosphorus
     export in winter.  Rapid nutrient turnover and high water hyacinth metab-
     olism apparently accounts for the low percentage of nitrogen and phosphor-
     us uptake. Seasonal changes in nitrate levels leaving the marsh suggest
     denitrification is a significant nitrogen sink, and sediments continue to
     act as an additional nitrogen source to overlying waters.  Use of a hya-
     cinth marsh as a natural tertiary treatment system  would require much
     lower nutrient loading rates to be effective.
603.  Mitsch, W.J., C.L. Dorge, and J.R. Wiemhoff.  1977.  Forested Wetlands  for
        Water Resources Management in Southern Illinois.  Research Rept.  132.
        Water Resources Center, Univ. of Illinois.  275 p.
604. Mitsch, W.J., C.L. Dorge, and J.R. Wiemhoff.   1979. Ecosystem Dynamics  and
        a Phosphorus Budget of an Alluvial Cypress  Swamp in Southern  Illinois.
        Ecology 60(6):1116-1124.

        Annual patterns in hydrology, phosphorus circulation, and sediment
     dynamics were studied in a southern Illinois,  USA floodplain swamp  domi-
     nated by bald cypress (Taxpdium distichum) and swamp tupelo (Nyssa  aguati-
     ca).  The study emphasized the swamp's interactions with the adjacent
     river.  For the year, major inputs of water to the swamp were throughfall
     (74.3 cm) and runoff (69.4 cm) with monir contributions due to groundwater
     (21.6 cm).  Outflows were by evapotranspiration  (72.3 cm), surface  outflow
     (56.5 cm), and groundwater (21.0 cm), with the latter two draining  primar-
     ily to the river.  A flood occurred during the study period, passing  1.6 x
     10^ m^ of river water over the swamp and depositing 0.06 cm of sedi-
     ments.
        An annual phosphorus budget was developed for the swamp from  field
     measurements.  The greatest input of phosphorus to the swamp was 3.6  g  P

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      m2 yr^ due to deposition  of high-phosphorus  sediments  during the
      flood.  This was 10 times greater  than  the outflow  of  phosphorus to the
      river.  0.34 g P m2 yr  ,  and 26 times greater  than  the throughfall
      input of 0.14 g P m2 yr1.  Total tree uptake from sediments was estimated
      to be 0.87 g P m2 yr1 of  which 0.77  g P m2 yr1  returns as  litterfall
      to the swamp sediments.   Duckweed  productivity  was  estimated to take 3.3 g
      P m2 yr1 from the water column and deposit this in  the sediments during
      die-off.
         For the period 1937-1967, cypress growth, based  on  tree ring analyses,
      was closely correlated with several  measures of flooding frequency and
      magnitude, all obtained from past  river data.   Tree ring data prior to
      1937 showed poor correlation with  flooding,  probably because of logging
      activity.  Cypress growth has decreased dramatically in recent years,
      corresponding to the rise in water level  caused by  beaver  activity.  (AA)
 605. Mitsch, W.J., M.D. Hutchison, and G.A. Paulson.   1979.   The Momence Wet-
         lands of Kankakee River in Illinois - An Assessment  of  Their Value.  A
         Descriptive and Economic Approach to the Appraisal of Natural Ecosystem
         Function.  State of Illinois, Institute of Natural Resources,  Chicago,
         IL, 55 pp.
 606. Mitsch, W.J., and K.C. Ewel.   1979.  Comparative Biomass  and Growth of
         Cypress in Florida Wetlands.  The Amer. Midi. Nat.  101(2):417-426.

         Tree biomass and increase in biomass were  determined for  cypress (Tax-
      odium distichum) in different  systems in Florida, USA.  Ten  trees  were
      harvested to determine biomass regressions.   Lowest  biomass  and tree
      growth rates were found in cypress-pine associations indicative of low
      water, in monospecific stands  of cypress which  are indicative of high
      water levels, and in a poorly  drained cypress dome.  Increases  in  indi-
      vidual tree growth rates were  found in cypress-tupelo  systems and  cypress-
      hardwood systems.  The latter  are less dominated by  cypress,  so individual
      tree growth is greater (7.7 kg/yr vs. 4.0 kg/yr).  Cypress-hardwood asso-
      ciations are generally better  drained than cypress-tupelo  sysems.   Two ex-
      perimental cypress domes currently receiving  treated sewage  effluent and
      groundwater showed high individual tree growth  (5.0  and 4.2  kg/yr,
      respectively), but little difference was noted  between the two  domes.  Tree
      diameter increase showed normal cypress tree  growth  to be  1.0-2.0  mm/yr
      with higher values of 2.8-3.3  in cypress-hardwood associations  and the
      experimental cypress dome.  Cypress in the poorly drained  dome  increased
      by only 2.0 mm/yr. (BA)
 607.  Mitsch, W.J.,  H.T. Odum, and K.C. Ewel.  1976.  Ecological Engineering
         Through the Disposal of Wastewater into Cypress Wetlands in Florida.
         National Conference on Environmental Engineering Research, Development
         and Design.  Univ. of Washington, Seattle.
1085.  Monge, D.  1978.

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608. Montroni, R.  1980.  The Importance of Riparian Zones to Terrestrial Wild-
        life; An Annotated Bibliography.  U.S. Fish and Wildlife Service.  Sac-
        ramento, CA.  83 pp.
609. Mood, E.W.  1976.  Epidemilogic and Public Health Implications of Wet-
        lands, pp. 116-120.  In; M.W. Lefor, W.C. Kennard, and T.B. Helfgott
        (eds.), Proceedings: Third Wetlands Conference.  Rept. No. 26. Insti-
        tute of Water Resources, Univ. of Connecticut, Storrs.

        This paper addresses two major concerns of medical and public health
     authorities about wetlands: (1) the use of subsurface absorption systems
     in or immediately adjacent to wetlands as a means of wastewater disposal;
     and (2) that wetlands provide breeding habitats for mosquitoes which are
     direct or indirect vectors of human disease (e.g., eastern equine enceph-
     alitis).  (DM)
610. Moore, J.W. 1979.  Diversity and Indicator Species as Measures of Water
        Pollution in a Subarctic Lake.  Hydrobiologia 66:73.
611. Moraine, R. et al.  1979. Algal Single Cell Protein from Wastewater Treat-
        ment and Renovation Process.  Biotech, and Bioeng., XXI:1191-1207.
612. Moreau, G.D. 1976.  Effects of Fertilizers on Aquatic Vegetation. Master's
        Thesis. Dept. of Wildlife Science, Utah State University, Logan. 40 pp.
613. Morel, F.M.M., and S.L. Schiff.  1980. Geochemistry of Municipal Waste in
        Coastal Waters. Rept. No. 259. Mass. Inst. of Tech. Cambridge.  205 pp.
614. Mosey, F.E. 1976. Assessment of the Maximum Concentration of Heavy Metals
        in Crude Sewage Which Will Not Inhibit the Anaerobic Digestion of
        Sludge.  Water Pollultion Control 75:10-20.
615. Motts, W.S. 1972. Environmental Impact of Alternative Waste Disposal Meth-
        ods.  Cooperative Extension Service. Univ. of Mass., Amherst, Mass.
616. Moulton, D.W, W.I. Jensen, and J.B. Low.  1976. Avian Botulism Epizootiol-
        ogy on Sewage Oxidation Ponds in Utah.  J. Wildl. Management
        40(4):735-742.

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        In  the microenvironment  concept of avian botulism epizootiology, it is
     hypothesized  that  invertebrate  carcasses may serve both as a substrate for
     toxin  production by Clpstridium bptulinum type C and as a vehicle for
     toxin  transmission to  water birds.  We field-tested that hypothesis by
     attempting  to induce botulism in wing-clipped mallard ducks (Anas platy-
     rhynchps) on  sewage oxidation ponds in Utah.  The experimental ponds were
     inoculated  with C.  bptju^immi spores in June 1974.  Aquatic insect popula-
     tions  were  monitored throughout the summer.  Rotenone was used in August
     to kill  insects in two ponds (one served as control), thereby providing
     potential substrate for clostridial growth and toxin production.  Botulism
     was not  detected among the  birds even though they routinely ingested in-
     vertebrate  carcasses.   Samples  of dead invertebrates contained no botu-
     linum  toxin.   We concluded  that the microenvironment concept, as  it now
     stands,  cannot always  be a  sufficient explanation of how type C botulism
     epizootics  are initated in  nature.  Other microbes may inhibit the growth
     of clostridial cells or destroy botulinum toxin.  (AA)
617. Mudroch, A. and J.A. Capobianco.   1978.  A Preliminary Study on a Natural
        Marsh Receiving Sewage Treatment Plant Effluent.   Canada Centre for In-
        land Waters, Geology  Section,  Process Research Division. Burlington,
        Ontario, Canada.  45  pp.

        Long-term effects of  poor  quality wastewater treatment plant effluent
     on a natural marsh  on the western shore  of Lake Ontario,  Canada, were
     studied.  The major contributor of N and P to the marsh area was the
     treatment plant discharge.  The effect of the effluent on plant communi-
     ties, and the availability and cycling of nutrients  and heavy metals in
     the marsh are discussed.  Metal concentrations in the water were generally
     low.  Increased concentrations of Pb, Cr, Zn, N, P,  and organic C were
     found in the sediment vertical profile obtained from the  marsh area.  The
     submersed aquatic plants collected from  the marsh area accumulated larger
     quantities of Pb, Zn, Cd, and Cr  than the same species collected at the
     station in the pond outside the marsh area.  The shoot standing crop of
     the dominant plant  species (Glyceria grandis) was associated with amounts
     of P, N, and organic C in the sediments.   (BA)
618. Mudroch, A., and J.A. Capobianco.   1979. Effects  of  Treated Effluent on a
        Natural Marsh.  J. Water Poll.  Control.  Fed. 51(9):2243-2256.

        Changes in biomass tissue nutrient  levels,  and tissues  metal  levels in
     emergent marsh vegetation as a  function of  increasing  distance from a sew-
     age discharge were studied at a  site in Ontario.   All  three parameters de-
     creased with increasing distance.
        Sediments were indicated as  the possible source of  heavy metals. Float-
     ing and submerged aquatic vascular plants adsorbed greater levels  of heavy
     methods than did emergent plants.   (EP)
619. Murnyak, M.O. and D.F. Murnyak.  1981. Aquaculture  in Minnesota's  Wetlands.
        pp. 51-58. In; B. Richardson  (ed.),  Selected Proceedings  of  the Midwest

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        Conference on Wetland Values and Management.  Minnesota Water Planning
        Board.  St. Paul.  660 pp.

        The feasibility of raising fish as a  source of  food in Minnesota  farm
     ponds is the focus of an ongoing research and extension project centered
     in Wright County, Minnesota.  Studies over the past three years have shown
     the farm ponds larger than 0.1 ac in size, averaging 4' or more in depth,
     can be used for food fish production on  a seasonal or year-round basis.
     Ideally, areas to be used for fish production should have a reliable
     source of good quality water, a controllable water level, and  allow  com-
     plete drainage. Lowland areas such as marshes and  swamps that  have abun-
     dant vegetation or are subject to flooding or greatly fluctuating water
     levels are not recommended for fish farming.  The choice of production
     method and species to culture is determined by the water quality condi-
     tions and the suitability of the site for intensive management.  Cold
     water ponds can be used for trout (Salmo spp.) production, where year-
     round growth is possible if flowing water is available.  Warm  water  ponds
     can be used for seasonal production of channel catfish (Ictalurus puncta-
     tus), bluegill sunfish (Lepgmis macrochirus), black crappies (Pomixis
     nigromaculatus), yellow bullheads (Ictalurus natalis) and largemouth bass
     (Micropterus salmoides).  Standing ponds averaging less than 15' deep are
     subject to winterkill, and must be completely harvested each year unless
     aeration is provided.  At this time it appears that using farm ponds for
     fish production in Minnesota is economical for home-use, but not for
     commercial operations.  (AA)
620. Murphy, C.B.  1981.  Bioaccumulation and Toxicity of Heavy Metals  and
        Related Trace Elements.  J. of Wat. Pollut. Control Fed.  53(6):993-998.
621. Murray, C.  1976. Weeds Hold Promise for Pollution Cleanup. Chemical  and
        Engineering News.  54(12):23-24.

        The water hyacinth is being tested as a means of  sewage  treatment  for
     small towns which cannot afford the capital investment necessary with con-
     ventional treatment techniques.  The water hyacinth  is a vascular  plant
     containing a system of vessels that transport nutrients from  the roots
     through the leaves while absorbing large quantities  of nutrients and
     pollutants found in domestic sewage.  The hyacinth also has the ability  to
     concentrate heavy metals and organic substances often present in indus-
     trial wastes.  National Aeronautics and Space Administration  (NASA) offi-
     cials have been conducting research at Bay St. Louis, Mississippi.  In the
     spring of 1975, three acres of a 40-acre municipal lagoon that receives
     raw sewage from about 6000 households was planted with water  hyacinths.
     Although three acres was insufficient to clean up the^entire  lagoon,
     planting 12 acres of the lagoon with hyacinths in summer of 1976 is
     expected to produce clean water in the lagoon.  Uses for water hyacinths
     produced are also being explored.  In a study at the Mississippi State
     University Agricultural Experiment Station, hyacinths grown in municipal
     sewage were dried, ground into meal, and added to cattle feed.  The hya-
     cinth meal is high in minerals and protein and comparable to  cottonseed
     meal or soybean meal as a feed supplement.  NASA is  researching the con-

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     version of water hyacinths  into  methane  by anaerobic  fermentation.  Residue
     produced by anaerobic  digestion  is  an  efficient  fertilizer  and soil condi-
     tioner 4
622. Nathans, M.W. and T.J.  Bechtel,  1977.   Availability of  Sediment-adsorbed
        Pesticides to Benthic with Particular  Emphasis  on Deposit-Feeding in
        Fauna.  U.S. Army  Corps Tech.  Rpt.  D-77-34.
623. National Academy of Sciences.  1976.  Making Aquatic Weeds  Useful:  Some
        Perspectives for Developing Countries.   Washington,  DC.,  174 pp.

        Aquatic weeds are becoming  a menace  at  alarming rates in  many parts of
     the world.  Several methods  for controlling these aquatic  macrophytes  are
     presented and discussed in semi-detail,  i.e.  utilization of  herbivorous
     animals, harvesting techniques, and  other  uses  such as  wastewater  treat-
     ment and aquatic plants for  food. Each  section  includes a  bibliography and
     a listing of research contacts.   (AA)
624. National Science Foundation.   1977. Putting Wetlands  to Work.   (Cypress
        Domes and Peatlands Offer Ecologically  Sound,  Energy-Conserving Waste-
        water Disposal).  NSF Mosaic 8(3):25-29.

        This article reprint reports on two  research projects,  conducted  in
     Florida and in Michigan, designed to ascertain the capability  of wetlands
     to absorb partially treated wastewater  from municipal facilities.  These
     studies are part of an accumulating body of research  that  suggests that
     wetlands are ecological systems that not only can absorb treated effluents
     while treating them in a natural rather than energy-consuming,  artificial
     way, but can benefit from the  fertilization secondarily treated effluents
     are able to provide.  The research suggests that  as long as  the effort is
     based on understanding of the  wetlands  environment, it represents  a  way
     that civilized man can return  to a symbiotic relationship  with  an  import-
     ant part of his natural environment.  There is substantial opportunity for
     small communities which occupy a large part of our geography to take ad-
     vantage of the results of these experiments.  (NT)
625. National Science Foundation. 1980.  Wetlands.  Our Natural Partners in
        Wastewater Management, 16mm Film in Sound and Color.  Directorate  for
        Engineering and Applied Science.  Washington, DC.
626. Natter, A.S., and D. Walrath.  1976.  Aquaculture - New Broom Cleans Up
        Wastewater.  Water and Wastes Engineering 13(2):38-41.

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627. Neary, D.G., G. Schneider, and D.P. White.  1975.  Boron Toxicity in Red
        Pine Following Municipal Wastewater Irrigation.  Soil Sci. Soc. of Am.
        Proc. 39:981-982.
628. Neel, J. K., and G. J. Hopkins.  1956.  Experimental Lagooning of Raw
        Sewage.  Sewage and Industrial Wastes 28(11):1326-1356.

        The authors outline studies being carried out at several places in
     U.S.A. on treatment of sewage by aquaculture systems, in which sewage is
     treated in lagoons. The problems of harvesting phytoplankton are overcome
     either by culturing filter-feeding shellfish to consume the phytoplank-
     ton, or by growing higher aquatic plants such as water hyacinth.  Flow
     diagrams of two proposed systems are included, and factors to be taken
     into account before such a scheme can be implemented are listed.  (AL)
629. Neff, J. W., R. S. Foster, and J. F. Slowey.  1978.  Availability of
        Sediment-Adsorbed Heavy Metals to Benthos with Particular Emphasis on
        Deposit-Feeding Infauna.  Tech. Rept. D-78-42.  U.S. Army Corps of
        Engineers Waterways Experiment Station, Environmental Laboratory.
        Vicksburg, MS.  311 pp.

        Biological laboratory studies were conducted to evaluate the bioavail-
     ability of sediment-adsorbed heavy metals to benthic invertebrates.  For
     these studies, five test organisms (Rangia cuneata, Palaemonetes
     kadiakensi s, Neanthes arenaceodentata, and Tubifex sp.) were exposed to
     metal-enriched natural sediments for periods up to six weeks at different
     salinities.  The test sediments came from Texas City and Corpus Christi,
     Texas, ship channels and Ashtabula, Ohio, harbor.  The accumulation of
     eight heavy metals (Cd, Cr, Cu, Fe, Mn, Ni, Pb, and Zn) by all species
     was measured.
        Statistically significant accumulation of metals from sediment was
     demonstrated only 36 times (26.5%) out of 136 metal-species-sediment test
     combinations.  Variations in bioaccumulation were observed between
     species, metals, sediments, and salinity.  In these studies, correlation
     was not observed between accumulation and specific metal forms as deter-
     mined by selective chemical extraction of test sediments.  Bulk metal
     analyses of the test sediments also did not correlate with metal bio-
     availability.  (AA)
630. Neil, J. H.  1976.  The Harvest of Biological Production as a Means  of
        Improving Effluents From Sewage Lagoons.  Canada-Ontario Agreement on
        Great Lakes Water Quality Research Rept. tt38.
631. Nelson, S.G., B.D. Smith, and B.R. Best,  1981.  Kinetics of Nitrate  and
        Ammonium Uptake by the Tropical Freshwater Macrophyte Pisjbia  stratiotes
        L. Aquaculture, 24 (1/2):11-19.

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        The kinetics of nitrogen uptake were  examined  for  a  common  freshwater
     macrophyte Fistia stratiotes L.  Nitrate-nitrogen and ammonium-nitrogen
     uptake were examined over a wide range of  substrate concentrations  gather
     information which would be useful in the design of aquaculture  systems  for
     ammonium and nitrate removal.  Nitrate uptake  rates were higher after 24
     hr. of exposure to the nitrate source than immediately  after exposure.  The
     rate of uptake of nitrate-nitrogen was greater in the light than in the
     dark.  Nitrate uptake followed a pattern which could  be adequately
     described by the Michaelis-Menten expression.  Ammonium nitrogen uptake
     response to substrate concentration appeared to be linear.  Rates of
     ammonium-nitrogen uptake was similar in  the dark  and  in the light.   For
     any given dissolved nitrogen concentration, the rate  of ammonium-nitrogen
     uptake was greater than the rate of nitrate-nitrogen  uptake.
632. Nessel, J. 1978.  Distribution and Dynamics of Organic Matter  and Phos-
        phorus in a Sewage Enriched Cypress Swamp.  Master's Thesis.  Depart-
        ment of Environmental Engineering Sciences, Univ. of Florida, Gaines—
        ville. 150 pp.
633. Nessel, J.K. , and S.E. Bailey.  1980. Distribution and Dynamics  of Organic
        Matter and Phosphorus in a Sewage-Enriched Cypress Swamp. In; H.E. Odum
        and K.C. Ewel (eds.), Cypress Wetlands  for Water Management,  Recycling
        and Conservation.  Fifth Annual Report  to NSF and Rockefeller Founda-
        tion, NSF Grant ttPFR-7706013, Rockefeller Foundation Grant #RF-76034.
634. Neuhold, J. M.  1971.  The Study of the Physical, Chemical, and Bio-
        logical Nature of Water Quality Under Utah Conditions.  Completion
        Rept. Utah State Univ., Logan, UT.  100 pp.

        Addition of domestic sewage effluent into a high mountain  valley
     stream can produce a complexity of effects on receiving waters.  Mountain
     streams are cold waters which have envolved a diverse biological system
     adapted to cold temperature, clear water and in which sewage  effluents
     can persist for relatively long periods of time.  The lower Logan River
     below the City of Logan is such a stream.  It receives domestic effluent
     from the City of Logan, which at the start of the study went  directly,
     untreated, into the Logan River, and during the course of the study was
     diverted through an extensive primary-secondary-tertiary treatment lagoon
     system returning treated water back to the receiving stream.  The phys-
     ical, chemical, and biological qualities of the Logan River were studied
     before and after treatment.  The development of a biological  system and
     its effect on the physical and chemical qualities in the lagoon system,
     built essentially upon a primordial substrate, were also studied.  An
     annotated bibliography of 250 references on wastewater stabilization
     ponds is included.  Tertiary treatment ponds can be ecologically managed
     to minimize organic build-up.  Design criteria should also be established
     to maximize retention time to fully utilize the ecosystem developed in
     the pond.   Free-form designs are suggested as a possible means to accom-
     plish this with the added benefit of improving aesthetics of  the problem.
     (NT)

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 635.  Nichols,  D.  S.   1980a.  Developing Environmentally Safe Procedures for
         Sewage Waste Treatment Using Organic Soils and Peat Materials.  Unpub-
         lished report.   U.S.  Forest Service, North Central Forest Experiment
         Station,  Grand Rapids, MN,  54 pp.
 636.  Nichols,  D.  S.   1980b.  Nutrient Removal from Wastewater by Wetlands. Paper
         presented at the 6th International Peat Congress, August 17-23, Duluth
         MN.   23 pp.

         Interest  in  wetland applications as a means of removing N and P from
      wastewater is rapidly increasing.   However, the capacity of wetlands to
      function in  this manner has not been well quantified.  This paper briefly
      reviews  the  literature on this topic.  The retention of inflowing P by
      wetlands under  natural conditions  seems to be limited to the relatively
      small  amount that is accumulated as peat is formed from partially decayed
      vegetation.   When P in excess of natural levels is added to a wetland,
      some is  adsorbed by the soil.  The efficiency with which wastewater P is
      removed  is highest at low loading rates and decreases rapidly as loading
      rates  increase.  Removal of P also declines with time; short-term studies
      can give misleadingly high estimates of ultimate P removal capacities.
      The removal  of  N in excess of the  natural rate of accumulation in the peat
      is apparently by denitrification.   As with P, the efficiency of N removal
      decreases rapidly as loading rates of wastewater N are increased.  The
      rate of  denitrification may be limited by the rate of nitrification or
      NH4~N, N03~N, or §2 diffusion.  From the sparse data available in
      the literature  it is estimated that  1 ha of wetland would be required to
      remove 75 percent of the P generated by 15 people and 75 percent of the N
      from 20  people, or 54 percent of the N and P generated by 50 people.  Wet-
      land application is feasible only where wetlands are abundant and popula-
      tion densities  are low. (AA)
 637.  Nichols,  D.S.  [No Date]  Capacity of Natural Wetlands to Remove Nutrients
         from Sewage.   U.S.  Forest Service, Grand Rapids, MN  24 pp. plus refer-
         ences  and illustrations.

         The mechanisms by which wetlands remove nutrients were reviewed.  A
      model of  the relationships between rates of wastewater application and
      efficiencies of  removal of N and P was developed on the basis of data from
      nine natural wetlands used for wastewater treatment: six located in the
      U.S., two located in Canada, and one located in Ireland.  It was concluded
      that natural wetlands can remove nutrients from wastewater effectively,
      but only if the  population density is relatively low and wetlands are
      relatively abundant.  Approximately 1 hectare of wetland was estimated to
      be required for  50% removal of nitrogen and phosphorus for every 60
      people.   An extensive (131 references) bibliography is included.  (EP)
1086.  Nichols, D.S.  1983.
 638.  Nichols, D.S. and D.H. Boelter. 1982.  Treatment of Secondary Sewage
         Effluent With a Peat-Sand Filter Bed.  J. Environ. Qual.  11(1):86-

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        A peat-sand filter bed was constructed  to provide  additional  treatment
     of the secondary sewage effluent from a campground  located  on  the  shore  of
     North Star Lake, within the Chippewa National Forest  in  north-central
     Minnesota.  This effluent contained an average of 30.9 mg/liter  total
     nitrogen, 8.63 mg/liter total phosphorus,  and 122,000 fecal coliforms/
     100 ml.  An average of 6.8 cm/week was applied from late May through early
     October for 8 years.  The peat-sand filter bed accomplished almost com-
     plete removal of fecal coliform bacteria and P.  About 90%  of  the  waste
     water N was removed during the 2nd and 3rd years of operation, but this
     declined to about 50% by the 5th year, due to oxidation  of  the peat and
     release of N.  The high Fe, Al, and ash content of  the peat contributed  to
     the filter's highly efficient removal of P.  Microbial immobilization  in
     the peat contributed to N and P removal during the  first 2  or  3  years  of
     waste water application.  Rough stalked bluegrass (Pqa triyialis L.)
     planted on the peat surface of the filter  was very  important in  overall  N
     and P removal.  Nutrient uptake by the bluegrass increased  each  year and
     accounted for 45% of the P and virtually all of the N removed  from the
     waste water in the 5th year of the study.  (AA)
639. Niering, W. A.  1972.  The Wetlands.  Ecol. Today 2:32-36.

        Four major roles of freshwater wetlands are discussed, including:
     hydrologic, productivity, pollution filtration, and education/recreation.
     Examples are cited from wetlands throughout the U.S.  Includes a  separate
     article on saltwater wetlands.  (DM)
640. Niering, W. A.  1973.  The Ecological Role of Inland Wetlands, pp.
        100-109 and 163-164.  In T. H. Helfgott, M. W. Lefor, and W. C.
        Kennard (eds.), Proceedings: First Wetlands Conference.  Rept. No.  21.
        Inst. Water Res., Univ. of Connecticut, Storrs.

        Five wetland types — marsh, wooded swamp, bog, floodplain, and stream
     belt ~ and their associated vegetation are discussed, followed by expla-
     nations of the numerous roles of wetlands including:  flood control,
     siltation or sediment trapping, recharging the water table, pollution
     filtration, oxygen production, productivity, aid in maintenance of a
     balanced nitrogen cycle, education, and recreation.  (DM)
641. Nixon, S. W., and C. A. Oviat.  1973.  Analysis of Local Variation in the
        Standing Crop of Spartirta alterniflora.  Botanica Marina  16:103-109.

        Measurements of standing crop biomass and height of the tall form of
     Spartina. alterniflora on 12 Rhode Island salt marshes suggests that the
     nutrients of municipal sewage inputs may increase cordgrass  growth.  Even
     with enhanced growth from eutrophication, calculations show  that treat-
     ment of marsh areas with sewage effluents is not a realistic hope for the
     recycling of wastes from coastal cities, or for providing low cost
     tertiary treatment.  (WE)

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642. Noble, R.  1975.  Growing Fish in Sewage.  New Scientist 67:259-261.
643. North Atlantic Treaty Organization.  1976.  Harvesting Polluted Waters:
        Waste Heat and Nutrient-Loaded Effluents in Aquaculture.  Plenum
        Press, New York.  335 pp.

        This symposium was concerned with the scope for making use of  the
     thermal pollution produced by power stations, and the waste minerals
     contained in domestic sewage, for the greater production of the primary
     and intermediate components in the Food chain of fresh and salt water
     fish.  Topics discussed covered the selection of sites where thermal
     "pollution" could be expected to produce beneficial results (e.g., fjords
     and nearly-closed bays), the idea of constructing "marine greenhouses,"
     the deliberate eutrophication of selected areas with attendant algal
     proliferation, the development of existing shellfish beds and the intro-
     duction of new species to heated areas, and the possibilities of  im-
     proving the rearing of fish fry for release and cultivation.  There is
     one paper drawing attention to the safeguards that would have to  be
     implemented when using domestic sewage for aquaculture.  (AL)
644. Notermans, S., A. H. Havelaar, and J. Schellart.   1980.  The Occurrence
        of ClostridjLum botulinum in Raw-Water Storage Areas and Their Elim-
        ination in Water Treatment Plants.  Water Research  14(11):1631-1635.

        Large scale outbreaks of waterfowl botulism in  1976 in the Netherlands
     have raised questions about the effectiveness of Clostridium botulinum
     removal by three water treatment plants.  The Leiduin, Weesperkarspel and
     Kralingen treatment plants each receive water from three storage areas
     where C. botulinum was detected in bottom mud samples.  The extent  of
     removal of the bacterium by routine treatment operations of the plants was
     examined, and model experiments were conducted to  evaluate the efficiency
     of slow sand filters in removing Clostridium spores.  Samples were  taken
     from coagulation sludge and the upper layers of filters in all three
     plants and also from the bottom sediments of ozone treatment chambers from
     the Weesperkarspel plant.  The presence of C. botulinum was determined by
     mouse antiserum bioassays.  Results of the three year investigations
     (1977-1979) indicated that slow sand filters were  adequate for retaining
     £•• sporogenes spores.  In two of the plants, the Weesperkarspel and Kral-
     ingen plants, C. botulinum was found only in the early purification
     stages.  At the Leiduin facility, C. bacteria were also detected on the
     slow sand filters, the last step of purification.  The bacterium was
     detected in only the upper sand layers of this plant, and was never found
     in the filter water of any of the three treatment  facilities.  (WR)
645. Nute, J. W.  1975.  Mt. View Sanitary District Marsh Enhancement Pilot
        Program.  Progress Rept. No. 1.  J. Warren Nute, Inc.  San Rafael, CA.
        9 pp.

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646. Nute, J. W.  1977.  Mt. View Sanitary District Marsh Enhancement Pilot
        Program.  Progress Report No. 2.  J. Warren Nute, Inc. Civil &  San-
        itary Engineers, San Rafael, CA.

        The data from the pilot marsh operation  for the period from January,
     1975 to July, 1976 indicate that secondary  effluent can be  used to create
     beneficial waterfowl habitat.  The pilot marsh areas established by Mt.
     View Sanitary District receive heavy use by waterflow and other birds.
     Studies begun in November, 1976 will provide additional data  on wildlife
     use of the marsh and its productivity for downstream habitats.
        Water quality analyses conducted thus far indicates that the marshes
     provide additional treatment to the effluent but that it will be necessary
     to control algae growth in order to consistently meet water quality objec-
     tives.  Control of algae blooms is particularly critical if receiving
     water objectives for undissociated ammonia  are to be met.
        Both achievement of water quality objectives and better  waterfowl habi-
     tat can probably be achieved by modifying the layout of the marsh  areas  to
     increase water management options.  This can be most easily done at Mt.
     View by expanding the pilot project to include Area C across  the drainage
     channel from the present areas.  This area  could be flooded and drained  as
     necessary for optimum plant growth and vector control.
        In conclusion, the pilot project thus far has yielded considerable in-
     formation necessary for the design and operation of a marsh for the bene-
     fit of wildlife.
647. Nute, J. W., and F. C. Demgen.   1977.  Marsh Enhancement Program -  Con-
        ceptual Plan.  Mt. View Sanitary District.  Contra Costa County, CA.
648.  Nute, J. W., and F. Demgen.  1979.  The Wetlands Wastewater Management
        Alternative.  In J. C. Sutherland and R. H. Kadlec  (eds.), Wetland
        Utilization for Management of Community Wastewater.  Abstracts  of a
        conference held 10-12 July 1979 at Higgins Lake, MI.  Mt. View,  CA.

        Mt. View Sanitary District, located east of Martinez, Contra  Costa
     County, California, within the San Francisco Bay metropolitan area, pro-
     vides secondary treatment for wastewater from a population  of approxi-
     mately 14,000 using the two-stage biofiltration process.  Since  1954 the
     District has developed a valuable wildlife habitat by  flooding nine acres
     of man-made wetlands adjacent to the plant with the treated effluent.  In
     1978 the wetlands system was expanded by construction  of three additional
     plots on ten additional acres.  This wastewater reuse project, providing
     valuable environmental benefits, has been developed by the  District as an
     alternative to disposal through a costly deep water outfall to Suisun  Bay.
        Environmental benefits of the wetlands system include improved  wildlife
     habitat, utilization of aquatic life forms to improve water quality and
     establishment of a valuable recreational and educational resource.  The
     wetlands system is managed with the objective of achieving  a flourishing
     wildlife habitat through use of nutrients in the wastewater to produce a
     living ecosystem of vegetation and aquatic organisms to be  utilized by
     higher forms of life for food and shelter.  Aquatic life forms also im-
     prove water quality by utilizing algae, detritus and organic pollutants

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     as food.  Through management, a balance  of  emergent  vegetation  and open
     water areas with adequate water circulation is  reached.  This is  important
     to attract migrating ducks who need vegetation  for food, protection and
     occasionally for nesting.  The District's freshwater wetlands supports 86
     species of birds, 20 species of animals, as well  as  33 species  of aquatic
     invertebrates.
        Flow through the wetland pond system  is  by gravity.  Thus, no  energy is
     required for moving or pumping the water.   Circulation and  mixing,  as  well
     as surface aeration, is provided by the  prevailing winds.   Solar  energy
     regulates the natural biologic processes developed within the wetlands
     ecosystem.  Artificial substrate, "Ecofloats" are installed in  open water
     areas to provide habitat for aquatic invertebrates near the surface of the
     water where oxygen levels are highest.
        Adequate water circulation and mosquito  fish (Gambusia afinis) are  the
     major means for controlling mosquito populations.  All ponds must be
     stocked with mosquito fish in the spring and maintained in  proportion  to
     the aquatic invertebrate population.   (AA)
649. Nute, J.W., and W.E. Nute.  1979.  Marsh/Forest Demonstration Project
        Feasibility Assessment.  J. Warren Nute, Inc. San Rafael, CA.  15 pp.
650. Oberts, G.L. 1981.  Impact of Wetlands on Watershed Water Quality,  pp.
        213-226.  In; B. Richardson  (ed.), Selected Proceedings  of  the Midwest
        Conference on Wetland Values and Management.  Minnesota  Water Planning
        Board.  St. Paul.  660 pp.

        A nonpoint source water quality sampling study was  conducted  on  six
     rural and 11 urban watersheds in the Minneapolis-St. Paul Metropolitan
     Area (2,968 square miles).  Wetland occurrence was one principal watershed
     selection criterion because wetlands were expected to  play  a major  role  in
     determination of watershed water quality.  Regionally, wetlands  comprise
     approximately 7.4 percent of the total seven-county area; wetland percent-
     ages in the sampled watersheds  vary from 4.8 to  16.3 in  the rural basins
     and from 0 to 14.5 in the urban basins.  Results of the  water  quality mon-
     itoring show that wetlands occurrence relates to the annual watershed
     loads for several sampled constituents.  Multiple regression statistical
     modeling of the sampled watersheds yields significant  relationships when
     various combinations of wetland-related watershed factors are  evaluated.
651. O'Brien, P., and J.W. Mitsch.  1980. Root Zone Nitrogen Simulation  Model
        for Land Application of Sewage Sludges. Ecological Modelling 8:233-257.

        A root zone nitrogen simulation model was designed to  evaluate long
     term effects of various sewage sludge and ammonia N fertilizer  applica-
     tions upon soil root zone N effects.  Specific effects studied  were root
     zone N build-up, crop N uptake (as a percentage of applied N),  crop yield
     and nitrate N leaching. Model results showed that nitrate N  leaching had  a
     direct linear relationship to the simulated sludge application  rates.  For
     each metric ton of sludge incorporated into the farm soils,  the leaching

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     rate increased 21 kg/ha/yr.  The leaching rate  increased 22  kg/ha/yr  for
     each metric ton of sludge incorporated into the stripraine soils.  Leaching
     rates 50% lower than incorporation amounts resulted  from surface
     application of sludge.  The maximum uptake of N was  determined  from
     Michaelis-Menten/Monod kinetics to be 135 kg/ha/yr for an incorporated
     loading rate of 25 metric tons/ha/yr.  Over a 50 year period organic  N  in
     the root zone build-up due to sludge application rates of 25 metric
     tons/ha/yr would range from 8,400 to 10,000 kg/ha, depending on the site.
     Simulated application of 300 kg/ha/yr of ammonia N gave a 98 kg/ha/yr crop
     N yield, representative of an average yield for the  U.S.  corn  belt.  Most
     of the model findings were in agreement with literature values.   (WR)
652. O'Brien, W. J.  1980.  Engineering Assessment: Use of Aquatic Plant
        Systems for Wastewater Treatment, pp. 63-80.  In S. C. Reed  and R. K.
        Bastian (eds.), Aquaculture Systems for Wastewater Treatment: An
        Engineering Assessment.  EPA-430/9-80-007.  U.S. Environmental Pro-
        tection Agncy, Office of Water Program Operations.  Municipal Con-
        struction Division.  Washington, DC.

        Aquaculture, the production of aquatic organisms under controlled
     conditions, has been practiced for many centuries to produce food, fiber,
     and fertilizer.  This legacy is both a boon and a liability when the
     feasibility for using aquatic plants in municipal wastewater treatment  is
     evaluated.  In the first place, the terms "aquaculture" and "aquatic
     plants" are much too broad to permit meaningful analysis of wastewater
     treatment systems unless boundary conditions are established.   The bound-
     aries used in this assessment are: 1) The aquatic plants used in the
     treatment processes are free floating macrophytes; 2) The primary object-
     ive of the treatment system is wastewater renovation.  Byproduct recovery
     is a useful adjunct to this objective but it is of secondary importance,
     and; 3) Aquatic plant treatment processes can be used to replace, or
     upgrade existing conventional treatment processes but they must
     successfully compete with these processes in terms of performance,
     reliability, and total costs.
        Another restriction imposed upon this assessment is limitation of the
     plant species to water hyacinth, Eichhornia cra s sipe s, and duckweed, Lemma
     sp.
653. O'Brien, W.J., 1981.  Use of Aquatic Macrophytes for Wastewater Treatment.
        J. of Environ. Eng. Div., Amer. Soc. of Civil Eng.  107(4):681-698.
654. O'Connor, D.J., R.V. Thomann, and D.M. DiToro,  1973.  Dynamic Water
        Quality Forecasting and Management. NTIS No. PB-225 048/8. Ecological
        Research Series Rpt. No. EPA-660/3-73-009.   August.

        The formulation and initial verification of  two modeling frameworks  are
     described.  The first is directed toward an analysis of the impact of the
     carbonaceous and nitrogenous components and wastewater on the dissolved
     oxygen resources of a natural water system.  The second modeling  framework

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     concentrates on the interactions between  the  discharge  of  nutrient,  both
     nitrogen and phosphorus, and the biomass  of the phytoplankton  and zoo-
     plankton populations which result, as well as  incorporating the  overall
     impact on dissolved oxygen.  The models are formulated  in  terms  of coupled
     differential equations which incorporate  both  the  effect of transport due
     to tidal motion and turbulence, and the kinetics which  describe  the  bio-
     logical and chemical transformations that can  occur.  The  modeling frame-
     works are applied to the Delaware and Potomac  estuaries in order to  esti-
     mate the ability of such models to describe the water quality  effects of
     carbon, nitrogen, and phosphorus discharges.   The  agreement achieved be-
     tween observation and calculation indicate that the major  features of the
     impact of wastewater components on eutrophication  phenomena can  be
     successfully analyzed within the context  of the models  presented.
655. Odens, D.  1976.  Suitability of Lagoon Effluents  for  Irrigation  in South
        Dakota.  Completion Rept. 1  (1 July 1975-3 June 1976)  to Office  of
        Water Research and Technology.  Dept. of Civil  Engineering,  South
        Dakota State Univ. Brookings, SD.  46 pp.

        An evaluation and classification of lagoon effluents in South  Dakota
     was made with respect to their  suitability for  irrigation.  An  estimate
     of the quantity of water available was also made.   Lagoon waters  from  20%
     of the total number of lagoons  were sampled and analyzed  during July and
     August.  The water quality of the lagoon waters was compared  to published
     water supply quality data for the respective water supply.  From  these
     comparisons,  empirical relationships were developed permitting  the  pre-
     diction of lagoon water quality on the basis of published water quality
     data.  Using these relationships, all lagoon waters were  classified with
     respect to their suitability for irrigation.  Estimates of volume of
     water available for irrigation  were based on area  of existing lagoons.
     (NT)
656. Odum, E. P.  1978a. Value of Wetlands as Domestic Ecosystems,  pp.  9-18.
        In J. H. Montanari and J. A. Kusler  (eds.), Proceedings  of  the
        National Wetland Protection Symposium held 6-8 June  1977 at Reston,
        VA.  FWS/OBS-78/97.  U.S. Fish and Wildlife Service, Washington,  DC.
657. Odum, E. P.  1978b. The Value of Wetlands: a Hierarchical Approach,
        pp. 16-25.  In P. E. Greeson, J. R. Clark, and J. E.  Clark (eds.),
        Wetland Functions and Values: The State of Our Understanding.   Pro-

        The value of wetlands is being recognized in political and economic
     circles; however, the means for quantifying values  for  decision purposes
     are yet to be developed.  The hierarchical approach to  determining the
     values involves a consideration of component levels (for example,  popula-
     tion), ecosystem levels, and global levels.  In ecosystems,  values are
     implied for hydrologic considerations and productivity.  Waste assimila-
     tion and the role of wetlands in global cycling and atmospheric stability
     form the highest level.  Several approaches for summing the  values that

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      accrue to the various hierarchical levels are possible, including the
      common denominator approach,  the scaling and weighting approach, and the
      replacemlent value approach.   The latter seems to be more acceptable to
      economists,  because one can make an accurate estimate of the monitary
      costs associated with the loss of a "free" service performed by a wetland
      ecosystem.   (AA)
 658.  Odum,  H.T.   1978.  Principals for Interfacing Wetlands with Development,
         pp.  29-56.   In;  M.  Drew (ed.), Environmental Quality Through Wetland
         Utilization.  Coordinating Council on Restoration of the Kissimmee
         River Valley and Taylor Creek-Hubbin Slough Basin.
1087.  Odum,  H.T.   In press.
 659.  Odum,  H.  T.,  and A.  F. Chestnut.  1970.  Studies of Marine Estuarine
         Ecosystems Developing With Treated Sewage Wastes.  Annual Report for
         1969-1970.  Accession No. PB 199537.  364 pp.
 660.  Odum,  H.  T.,  A.  F.  Chestnut, M. Beeston, J. Day, and C. R. Dillon.  1970.
         Studies of Marine Estuarine Ecosystems Developing with Treated Sewage
         Wastes.  Annual Rept.   1969-1970.  Institute of Marine Sciences.
         Univ.  of North Carolina, Morehead City, NC.  366 pp.

         The report concerns developments in a study of ecologial systems that
      develop when the treated wastes from municipal sewage systems flow into
      estuarine waters.   Studies by a team of faculty and students of the
      University of North Carolina are considering a small marsh-lined estuary,
      Calico Creek,  which receives the wastes following secondary sewage treat-
      ment and a set of three ponds in which estuarine water and treated sewage
      mixture flows.  Three control ponds receive tap water and estuarine
      water.  Now in their second year, the ponds are rich in productivity with
      successive algal blooms throughout the year and a food chain culminating
      in blue crabs.  In this report there are chapters on the events in the
      salinity regime  and input management, photosynthetic productivity and
      respiration,  algal growth, P and N, bacteria, and animal populations.
      The presence of  a substantial ecological system suggests a viable inter-
      mediary system interface is possible between man's municipal wastes and
      normal estuaries.   These systems have potential for aquaculture and waste
      amelioration.   (AA)
 661.  Odum,  H.  T.,  and S.  Brown.   1976.   Regional Implications of Sewage
         Effluent Application on  Cypress Domes,  pp. 329-330.  Tn D. L. Tilton,
         R.  H.  Kadlec,  and J. C.  Richardson (eds.), Proceedings of a National
         Symposium  on Freshwater  Wetlands and Sewage Effluent Disposal.  Univ.
         of  Michigan, Ann  Arbor.

         The regional balance of  swamps and man are considered for wetlands
      districts of  Florida using  models, simulations,  energy evaluations,
      energy quality, measurements of water, and the investment ratio prin-

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     ciple.  Five special examples given include a long range model of basin
     formation and landscape maintenance; record of sewage in swamps at
     Wildwood (FL); development density and water priority with energy in-
     vestment ratio; contracting water and energy quality; regional model of
     water, energy, land use, and nutrients in the green swamp district of
     Florida.  (AA)
662. Odum, H. T., K. Ewel, W. Mitsch, and J. Ordway.  1975.  Recycling Treated
        Sewage Through Cypress Wetlands in Florida.  Occasional Publ. No.  1.
        Center for Wetlands.  Univ. of Florida, Gainesville.
663. Odum, H. T., and K. C. Ewel.  1978.  Cypress Wetlands for Water Manage-
        ment, Recycling and Conservation.  Fourth annual report, Center  for
        Wetlands, University of Florida, Gainesville.  953 p.

        Recycling of treated sewage wastewaters into two cypress domes in
     Gainesville, Florida is in its fourth year with much accelerated growth
     of cypress trees and recharge of groundwater after removal of most  of the
     nutrients and microbes by the natural filter mechanisms of the swamps.
     The cypress swamps through the shielding effect of their thin leaf  cover
     and their leaf drop in dry season save water, as compared to open water
     storage.  Extension of studies to a cypress strand at Waldo receiving
     sewage for 43 years showed the long-term stability of the ecosystem
     receiving the nutrients.  Drawdown of superficial groundwaters was  found
     to occur a half mile from canals in South Florida.  Lowering the water
     tables reduced the productivity and temporarily reduced transpiration.
     Evaluations showed that cypress swamp substitution for technological
     tertiary treatment was feasible.  (NT)

664. Odum, H. T., and K. C. Ewel (eds.).  1980.  Cypress Wetlands for Water
        Management, Recycling and Conservation.  Fifth and Final Report.
        Center for Wetlands, Univ. of Florida, Gainesville.  291 pp.

        This is the final report to National Science Foundation and the  Rocke-
     feller Foundation on results of research grant: "Cypress Wetlands for
     Water Management, Recycling, and Conservation."  Results given in the
     first four project reports are not repeated, but new data obtained  in
     1977-1979 are included.  Also included are summaries of the results and
     implications for management.  A book entitled Cypress Swamps reporting all
     results is being prepared for publication for the Center and is forthcom-
     ing from University Presses of Florida.
        Recycling of treated wastewaters in cypress domes has now had 5  years
     of testing and appears to be feasible.  This method is preferable to tech-
     nological treatment because it costs less; it is better than release on
     uplands because better filtration exists and less ecosystem change  is
     required; and it is preferred to release in open waters because the swamp
     ecosystem adapts better to high nutrients and low oxygen conditions.  In
     Florida, workshops and discussions with state authorities have led  to con-
     sidering this alternative for tertiary treatment and for storm runoff on a
     case-by-case basis.

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        A surprising discovery is the savings of water from evapotranspiration
     by upland swamps as compared to open water.  Comparative studies show that
     wetlands1 photosynthetic productivity ranged from very low to very high
     depending mainly on the amount of flowing water and the nutrients in the
     trajectory.  Energy evaluations show the swamps to be a major way to both
     save energy and use solar energy for useful economic purpose, thereby sav-
     ing waste treatment costs and maintaining a supply of high quality timber.
        A series of smaller projects has been initiated with the National
     Science Foundation and other agencies to prove long-term feasibility, to
     determine the best loading for wastewater addition to flowing swamps, and
     to test other kinds of wastewater, such as those containing sulfates and
     those from phosphorus mining.  The Center for Wetlands, which has started
     with funds from this project, has recently received an increased level of
     funding from the university.  A variety of projects on mangroves, marshes,
     and other swamps continues as significant research activities aimed at
     increasing our understanding of the basic and applied aspects of wetlands
     and their symbiosis with humanity.  Other work considers regional aspects
     of water and energy of wetlands.
        The unburned cypress pond receiving treated sewage wastes continues to
     show healthy tree growth, to maintain a layer of duckweed most of the
     year, and to allow fast growth of planted seedlings where light is ade-
     quate.  In order to test for self-regeneration and long-range management
     options, sewage flow to the burned pond was eliminated 2 years ago. Seed-
     ling regeneration has not yet occurred.  Seed supply is adequate but ex-
     ceptionally high waters in what is usually the dry period of both years
     prevented germination or drowned the seedlings.
        The conditions necessary for substantial natural regeneration and their
     frequency have yet to be established.  Funds obtained from Environmental
     Protection Agency allow continuation of the experiment for another year
     beyond the NSF-Rockefeller projects, and other funds are being sought so
     that the means to establish long-range sustenance of wetland ecosystems
     can be determined.  (AA)
665. Odum, H.T., K.C. Ewel, W.J. Mitsch and J.W. Ordway.   1977.   Recycling
        Treated Sewage Through Cypress Wetlands in Florida, pp.  35-67.   In;
        F.M. D'ltri  (ed.), Wastewater Renovation and Reuse. Marcel Dekker, New
        York.
666. Odum, H. T., K. C. Ewel, J. W. Ordway, and M. K. Johnston.   1975.   Cy-
        press Wetlands for Water Management, Recycling and Conservation.
        Second Annual Report.  Center for Wetlands, Univ. of Florida, Gaines-
        ville.  817 pp.

        Studies of water, nutrients, geological process, chemical  cycles,
     microbes, vegetation, forestry, wildlife, economics, energetics, and
     systems are being made in field tests and in comparisons with other swamp
     sites.  Treated sewage from a trailer court is flowing at  1  inch per week
     into two cypress domes, one severely burned and one unburned.   The  burned
     sewage dome, originally perforated with soil pits and water-jetted  wells,
     leaked some coliform bacteria and virus into the groundwater  in the first
     year.  Now, in its second year, it is retaining nutrients, heavy metals

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     and micro-organisms.  Both sewage domes have anaerobic water and a heavy
     cover of duckweed, but decomposition of organic matter in this semi-na-
     tural anaerobic digester is as fast as in aerobic domes.  So far, the
     cypress swamps seem to be a feasible alternative for wastewater re-
     cycling, using the semi-natural interface swamp ecosystems for improving
     water quality and recharging groundwaters.  (NT)
667. Odum, H. T., K. C. Ewel, J. W. Ordway, and M. K. Johnston (eds.).  1976.
        Cypress Wetlands for Water Management, Recycling, and Conservation,
        Third Annual Report.  Center for Wetlands, Univ. of Florida.  Gaines-
        ville.  879 pp.

       This is the third annual report on the Cypress Wetlands project spon-
     sored by the Rockefeller Foundation and the RANK Division of the National
     Sciences Foundation.  Studies of water budget and geology show how cy-
     press domes store and save water compared to reservoirs.  Experiments
     with recycling treated sewage water show that the cypress basins (domes)
     filter nutrients, microbes, heavy metals, and virus, improving the water
     as the sewage water recharges groundwater.  Measurements and systems
     models show budgets of phosphorus, nitrogen, and carbon, and the role of
     trees, shrubs, sediments, duckweed, and sedimentation in processing
     materials, productivity, total metabolism, and building ecological
     patterns.  Comparisons are made with strands and floodplains.  Regional
     studies consider the quantitative role of swamps in water and nutrient
     budgets of four counties and the south Florida region.  (NT)
668. Odum, H. T., K. C. Ewel, J. W. Ordway, M. K. Johnston, and W. J. Mitsch.
        1974.  Cypress Wetlands for Water Management Recycling and Conserva-
        tion.  First Annual Report. Center for Wetlands, Univ. of Florida,
        Gainesville. 947 pp.
669. Okorie, P. E.  1976.  Effect of Sewage Effluent in Cypress Ponds on
        Surrounding Soil Moisture and Nutrient Content and on Growth of Pine
        Trees, pp. 438-472.  In H. T. Odum, K. C. Ewel, J. W. Ordway, and M.
        K. Johnston (eds.). Cypress Wetlands for Water Management, Recycling,
        and Conservation.  Third Annual Report.  Center for Wetlands.  Univ.
        of Florida.  Gainesville.
670. Ordway, J. W. 1976a.  General Costs for Cypress Wetland Recycling.  In H.
        T. Odum, K. C. Ewel, J. W. Ordway, and M. K. Johnston (eds.), Cypress
        Wetlands for Water Management, Recycling, and Conservation.  Third
        Annual Report.  Center for Wetlands.  Univ. of Florida.  Gainesville.
671. Ordway, J. W. 1976b.  General Site Selection, Design Criteria, and Costs
        for Recycling Wastewaters through Cypress Wetlands.  Master's Thesis.
        Univ. of Florida, Gainesville, 54 pp.

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672. Ordway, J. W. 1976c.  New phases:  Waldo, a Strand Receiving Sewage,
        pp. 803-811.  In H. T. Odum, K. C. Ewel, J. W. Ordway, and M. K.
        Johnston  (eds.), Cypress Wetlands for Water Management, Recycling, and
        Conservation, Third Annual Rept.  Center for Wetlands.  Univ. of
        Florida, Gainesville.
673. Ordway, J.W.  1976d.  The Projected Cost of Cypress Wetland Disposal for
        Waldo, Florida,  pp. 803-811.  In; Third Annual Report on Cypress Wet-
        lands, University of Florida, Center for Wetlands, Gainesville.

        The general costs for using cypress wetlands as recycling sites for
     secondarily treated sewage are considered for the city of Waldo, Florida.
     Using one preliminary design, the total cost for this wetland treatment
     site was 42.2 cents per 1000 gallons compared with 62 cents per 1000
     gallons for spray irrigation and 106 cents per 1000 gallons for advanced
     waste treatment.
674. Ornes, W.H., and D.L. Button.  1975.  Removal of Phosphorus from Static
        Sewage Effluent by Water Hyacinth.  Hyacinth Control J. 13:56-58.
675. Ornes, W.H. and K.K. Steward, 1973.  Effect of Phosphorus and Potassium on
        Phytoplankton Populations in Field Enclosures.  Agricultural Research
        Service, Ft. Lauderdale, FL.  NTIS No. PB-231 650.  May 14 p.

        Some of the parameters involved in the addition of nutrients to a marsh
     environment are evaluated.  The effect of weekly applications of phosphor-
     us and potassium on algal populations in artificially isolated segments
     of a natural marsh environment was monitored.  Applications were made to
     field enclosures for 22 weeks.  The treatment rates were equivalent to the
     quantities contained in 2.5 cm/ha of sewage effluent with a concentration
     of 10 mg/liter P and 20 mg/liter K.  The rates were arbitrarily chosen and
     were similar to rates recommended for terrestrial application. Phytoplank-
     ton blooms and dynamic shifts in dominant phytoplankton genera resulted.
     Also, the aquatic macrophytes Chara and Utricularia disappeared after 22
     weeks of treatment.  Both polluted and clean water phytoplankton genera
     appeared in the control and treated enclosures.  The experiment results
     indicated a disrupted environment.  More study should be conducted before
     utilizing the sawgrass marshes as living filters for wastewater.
676. Oron, G. et al.  1979.  Algae/bacteria Ratio in High-rate Ponds Used for
        Waste Treatment.  Applied and Environ. Microbio., 38(4):570-576.
677. Oswald, W.J. 1973.  Productivity of Algae in Sewage Disposal.  Solar
        Energy, 15: 107-117.

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678. Oswald, W.J. et al.   1953.  Algal Symbiosis  in Oxidation Ponds.   II.
        Growth Characteristics of Chlprella pyrenoidosa culture in  sewage.
        Sewage Ind. Wastes, 25: 26-37.
679. Oswald, W.J. and H.B. Gotaas.   1957.  Photosynthesis in Sewage Treatment
        Trans. Am. Soc. Civ. Eng. Eng.,  122:73-105.
680. Ozimek, T.  1978.  Effect of Municipal Sewage on the Submerged Macro-
        phytes of a Lake Littoral.  Ekologia Polska 26:3-39.
681. Palmer, H. V. R.  1971.  Research Project Appears to Hold Special Promise
        for Towns, Fishing.  National Fisherman.  Camden, ME.  52(5):20-24.
682. Palmer, M., and S. T. R. Espirito.  1980.  Environmental Study of the
        Tagus Estuary.  Nature and Resources - UNESCO 16(3):14-21.

        The Tagus Estuary of Portugal has been severely degraded by the
     introduction of industrial and municipal wastes and nonpoint source
     pollution contributions.  A research project was organized to document
     the extent of estuarine pollution, its impacts on the environment and
     industry, and remedial measures.  The estuary is an important regional
     resource, as it is used for transport, aquaculture, urban and agricul-
     tural water supply, and recreation.  (EL)
683. Parizek, R. R.  1973.  Site Selection Criteria for Wastewater Disposal -
        Soils and Hydrogeologic Consideration, pp. 95-147.  In W. E. Sopper
        and L.T. Kardos (eds.), Recycling Treated Municipal Wastewater and
        Sludge Through Forest and Cropland.  Pennsylvania State University
        Press.
684. Parker, C.D. and G.P. Skerry, 1968.  Function of Solids in Anaerobic
        Lagoon Treatment of Wastewater: J. Water Pollut. Control Fed. 40(2):
        192-204.

        Field studies of anaerobic lagoons in Australia have shown that solids
     farther from the inlet of a lagoon are more active in purification than
     those deposited closer to the inlet, although in many cases they show
     lower gas yields.  Lagoon sludge purification index (Ibs BOD/acre/day/lb
     vs) values ranged from 0.0053 during the winter at a typical pond inlet to
     0.00902 during the summer at the pond outlet.  The organic load to the
     pond during this period was about 482 Ibs BOD/day/acre, and the sludge gas
     yield varied from 1.2 to 7.7 ml/day/gvs.  The presence of algae in pond
     supernatant apparently does not reduce the sludge's BOD removal capacity.

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     Where methane fermentation is inhibited, BOD can still be removed by sul-
     fate reduction.  Laboratory studies showed that a substantial increase
     (almost 30%) in BOD removal can be achieved by mixing sludge with the
     supernatant, and that algae proliferate readily in media with high organic
     contents.
685. Parker, D. S.  1976.  Performance of Alternative Algae Removal Systems.
        In E. F. Gloyna  (ed.), Ponds as a Wastewater Treatment Alternative.
        Center for Research  in Water Resources.  Water Resources Symposium No.
        9.  Univ. of Texas Press, Austin.
686. Parker, P. E.  1974.  A Dynamic Ecosystem Simulator.  Ph.D. Diss. Univ.
        of Michigan, Ann Arbor.
687. Parker, P. E., P. K. Gupta, K. R. Dixon, R. H. Kadlec, and D. E. Hammer.
        1978.  REBUS: A Computer Routine for Predictive Simulation of Wetland
        Ecosystems.  Report to the National Science Foundation.  NSF
        ENV 76-80708.  Univ. of Michigan, Ann Arbor.
688. Parker, P. E., and R. H. Kadlec.  1978.  A Dynamic Ecosystem Simulator.
        Paper presented at the American Institute of Chemical Engineers 78th
        National Meeting, Salt Lake City, UT.  August 1978.
689. Parrott, H. A., and D. H. Boelter.  1977.  The Use of Peat Filter Beds
        for Wastewater Renovation at Forest Recreation Areas.  Presented at a
        Symposium on Municipal Wastewater and Sludge Recycling on Forest Land
        and Disturbed Land.  Philadelphia, PA.
690. Patel, P. M., A. Wallace, W. L. Berry, and O. R. Lunt.  1978.  Reclaimed
        Sewage Water: A Hydroponic Growth Medium for Plants.  Resource
        Recovery and Conservation 3(2):191-199.

        Crops of cucumber, tomato, lettuce and chrysanthemum have been grown
     in flow culture with secondary sewage effluent as the nutrient source.
     Energy was saved because fertilizers were not necessary.  Cu, Zn, Mn, Fe,
     Ni, and Pb were removed from the water by the plants so that it could be
     used for other biologically-based processes where heavy metals might
     cause problems.  Variable amounts of nitrates and phosphates were
     stripped from the water, and these amounts could be increased at the
     expense of crop production.  (AL)

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691. Patrick, R.  1976.  The Role of Aquatic Plants in Aquatic Ecosystems,  pp.
        53-59.  In;  J. Tourbier and R.W. Pierson, Jr. (eds.), Biological
        Control of Water Pollution. Univ. of Pennsylvania Press, Philadelphia.

        Aquatic plants enrich aquatic and wetland ecosystems by fixing carbon
     and giving off free oxygen during photosynthesis.  They also provide shel-
     ter for aquatic organisms.  In the growth of aquatic plants various nutri-
     ents are removed from the water.  Nutrients may be accumulated in excess
     of what is needed for growth.  Significant amounts of heavy metals are
     also accumulated.  Nutrients and trace metals are stored in plant tissues
     and are returned to the aquatic ecosystem as food or detritus.  In the
     case of emergent vegetation, they may be dispersed into the terrestrial
     ecosystem.  Wetlands are important for the production of various sub-
     stances essential for the aquatic ecosystem.  An example is the production
     of vitamin B12 by fungi and bacteria in swamps.
692. Patrick, W.H., Jr., and R.D. Delaune.  1976. Nitrogen and Phosphorus Util-
        ization by Spartina alterniflora in a Salt Marsh in Barataria Bay,
        Louisiana.  Estuarine and Coastal Marine Sci. 4:59-64.
693. Patrick, W.H., R.D. Deluane, D.A. Antie, and R.M. Engler.   1971.  Nitrate
        Removal from Water at the Water-Soil Interface in Swamps, Marshes,  and
        Flooded Soils.  Annual Progress Report PFWOA, EPA (Project  1605 FJR,
        LSU).
694. Patrick W.H., Jr., R.D. Deluane, R.M. Engle, and S. Gotoh.   1976. Nitrate
        Removal from Water at the Watermud Interface in Wetlands.  U.S. Envi-
        ronmental Protection Agency. Ecological Research Series.  Washington, DC

        Flooded swamp and coastal marsh soils in Louisiana have a high capacity
     for nitrate reduction, according to reserarch focused:  (1) on learning how
     rapidly, how completely and by what mechanism nitrate is removed by bio-
     logical reduction from shallow surface water in swamps, marshes and
     flooded soils, and (2) to determine the oxidation-reduction  properties of
     the water-mud interface that control or influence the reduction of nitrate
     to nitrogen gas.  Oxidized and reduced layers in flooded soil were differ-
     entiated according to the vertical distribution of the  oxidation-reduction
     (redox) potential and concentrations of manganous manganese, ferrous  iron,
     sulfide, nitrate and ammonium.  Redox potential was measured with a
     special motor-driven assembly which pushed a platinum electrode through a
     soil sample at a steady rate.  From laboratory experiments,  it was found
     that nitrate added to shallow floodwater rapidly disappeared, oxygen  loss
     from sediments occurred rapidly and nitrate reduction was not inhibited by
     up to 16 ppm oxygen dissolved in soil suspensions.  The nitrate was not
     reduced in the floodwater but filtered downward into the anaerobic soil
     layer.  Additions of organic matter to a mineral soil flood  for rice
     culture decreased the thickness of the aerobic-anaerobic zone at the  soil-
     water interface and increased the rate of nitrate reduction.

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695. Patterson, D. R.  1971.  Impact of Effluent Spraying on the Small Mammal
        Populations and Vegetation of the Disposal Area.  Master's Thesis.
        Bowling Green State University, Bowling Green, OH.

        To determine the effect of effluent spraying on the small mammal
     populations and vegetation of the disposal area, two different systems
     were selected for study, a woodland disposal system at Seabrook Farms,
     Seabrook, New Jersey, and a grassland disposal system at the Campbell
     Soup Company, Napolean, Ohio.  Periodic systematic surveys of the vege-
     tation and small mammals had been undertaken by other investigators at
     Seabrook since the start of the effluent spraying system in 1950.  The
     present study deals with data previously collected by others as well as
     1967 and 1969 data collected at Seabrook and 1968-69 data collected at
     Napoleon by the author.
        For the first two or three years of effluent spraying at Seabrook, the
     mixed-oak woods exhibited an increased rate of growth over reference
     areas, however, those trees sprayed more than three years began dying,
     presumably by defoliation and debarking of the trees by the sprayers,
     thus opening them up to fungal and insect infestation.  A decrease in
     Perpmyscus leucopus populations increased in marginally sprayed areas,
     where the force of the spray was reduced by distance and a dense her-
     baceous cover which provided cover and a variety and quantity of food.
     The increase in Perpmyscus populations in the marginally sprayed areas
     may be due to higher pregnancy rates, or invasion of mice into the more
     favorable habitat.  The data from the Campbell spray fields is somewhat
     inconclusive with only two years of data.  (AA)



696. Patterson, J.W., R.A. Minear, and T.K. Nedved.  1971.  Septic Tanks in the
        Environment.  NTIS, Springfield, VA.
697. Pavoni, J. L., S. W. Kelber, and G. T. Bablitt.  1974.  The Harvesting of
        Algae as a Food Source From Wastewater Using Natural and Induced
        Flocculation Techniques, pp. 435-497.  In Wastewater Use in the Pro-
        duction of Food and Fiber- Proc.  U. S. Environmental Protection
        Agency.  Ada, OK.
698. Payonk, P.M.  1975. The Response of Three Species of Marsh Macrophytes to
        Artificial Enrichment at Dulac, LA.  Master's Thesis.  Louisiana State
        University.  Baton Rouge.  122 pp.

        This study details the response of three emergent species to treatment
     with wastewater from a menhaden processing plant.  The wastewater signi-
     ficantly increased net above-ground productivity of all species, 38 -
     51%.  Percentage of applied nutrients actually incorporated into plant
     tissue was measured.  Values ranged from 3.6 - 41.1% for N, and
     4.0 - 7.4% for P. Applied nutrients accounted for a 12 - 16% increase in
     N and P in surface waters of the marsh.  The remaining nutrients were
     accounted for through incorporation into the marsh's soil system, as
     through limited flushing action.  (WE)

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699. Payonk, P.M., R.E. Turner and J.H. Stone.  1976. Production, Tissue Nutri-
        ents and the Response to Enrichment of Three Marsh Macrophytes in
        Louisiana,  unpbl. manuscript.
700. Pecor, C. H., J. R. Novy, K. E. Childs, and R. A. Powers.  1973.
        Houghton Lake Annual Nitrogen and Phosphorus Budgets.  Tech. Bull. No.
        73-6.  Michigan Dept. Nat. Res., Lansing.  128 pp.
701. Perry, J.J., D.E. Armstrong, and D.D. Huff.  1981. Phosphorus Fluxes  in an
        Urban Marsh During Runoff, pp. 199-212.  In; B. Richardson (ed.),
        Selected Proceedings of the Midwest Conference on Wetlands Values  and
        Management.  Minnesota Water Planning Board.  St. Paul.  660 pp.

        Marshes have frequently been regarded as filters that remove nutrients
     from water passing through them.  A field-oriented study was done  to  show
     the effects of an urban marsh on the transport of dissolved and particu-
     late P during runoff events.  The marsh was particularly amenable  to  eval-
     uation because a water budget had been developed for the area and  90% of
     the water enters the marsh as residential runoff from a gaged storm sewer.
        The basic approach involved direct measurement of P input from  runoff,
     precipitation, and groundwater recharge. P output was obtained from P con-
     centrations in surface and groundwater discharge.  Laboratory and  field
     investigations were used to calculate the extent of P flux in the  marsh
     by the mechanisms of particulate sedimentation, soil sorption and  leaching
     from vegetation.
        The annual dissolved P input was 224 Kg.  Approximately 14% of  the
     dissolved P was retained in the marsh, primarily by the mechanisms of
     sorption during filtration.  Seasonal variability in dissolved P retention
     (8-50%) reflects the capacity of the runoff water to infiltrate the marsh
     soils.  Annual particulate P input was 282 Kg  of which 82% was retained by
     particulate sedimentation.  These values do not include release of partic-
     ulate P originating from the erosion of marsh  soils.  Reservation  should
     be used in extrapolating the data to other wetlands owing to the unique
     hydrology in Wingra marsh.   (AA)
702. Pershe, E. R.   1980.  Combined Aquaculture  Systems  for  Wastewater Treat-
        ment in Cold Climates,  an Engineering Assessment,  pp.  105-127.  In S.
        C. Reed and  R. K. Bastian  (eds.),  Aquaculture  Systems  for Wastewater
        Treatment: An Engineering Assessment.  EPA-430/9-80-007.   U.S. Envir-
        onmental Protection Agency.   Office  of Water Program Operations,
        Municipal Construction  Division.   Washington,  DC.

        The purpose  of this assessment  is  to highlight some  of the more impor-
     tant aspects of combined aquaculture  systems  and  to evaluate their merit
     for use in cold climates.   Much  of the  assessment is based on presenta-
     tions that were made at  a  seminar  held  on the campus of the University of
     California at Davis  in September 1979.

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703. Peters, R.E. and C.R. Lee.  1978.  The Potential of Overland Flow to Treat
        Runoff Water in the Kissimmee River Valley and Taylor Creek-Nubbin
        Slough Watersheds, pp. 202-216.  In; M.A. Drew (eds.), Environmental
        Quality Through Wetlands Utilization.  Coordinating Council on the
        Restoration of the Kissimmee River Valley and Taylor Creek-Nubbin
        Slough Basin.  Tallahassee, FL.  243 pp.

        Overland flow has been used successfully to treat both industrial and
     municipal wastewaters for a number of years.  Wastewater renovation is
     achieved through interaction of the physical, chemical, and biological
     components of the grass/soil system.  The most notable example of indus-
     trial application in the United States is the use of overland flow to
     treat cannery wastewater by the Campbell's Soup Company in Paris, Texas.
        As part of the mandate in Public Law 92-500 the U.S. Army Corps of
     Engineers has conducted extensive research on land treatment of wastewater
     to develop design and operational criteria for cost-effective wastewater
     treatment systems.  A field research facility to investigate overland flow
     for treatment of municipal wastewater was established near Utica, Mississ-
     ippi, in the fall of 1975.  Results indicate that overland flow can reduce
     applied nitrogen concentrations of 20 mg/1 to below 5 mg/1, representing  a
     mass nitrogen reduction of up to 90%.  However, only moderate reduction of
     phosphorus has been achieved.  BOD and suspended solids have been reduced
     to 10 and 15 mg/1, respectively.
        Overland flow has potential for reducing pollutants from both point and
     nonpoint sources in the Kissimmee River watershed. Three alternatives
     appear to have the most potential:

        a.  Effluents from dairy stabilization ponds or lagoons may be spray or
            flood irrigated onto adjacent pasture for overland flow treatment.

        b.  Storm-water runoff from improved pasture, citrus groves, or other
            agriculturally oriented areas may be collected in detention ponds
            and spray or flood irrigated onto adjacent lands at a lower, con-
            trolled rate to enhance nutrient removal and water use by agricul-
            tural crops.

        c.  In lieu of storm water detention, a third alternative is to build  a
            broad, base terrace at near zero channel grade to promote infiltra-
            tion and evapotranspiration of storm water runoff where soil condi-
            tions permit.
704. Peverly, J.H.  1982.  Stream Transport of Nutrients Through a Wetland.  J.
        of Environ. Qual.  11:38-42.

        The potential of managed wetlands to remove nutrients from agricultural
     drainage water was investigated by hydrographic and nutrient analyses for
     2 years in Oak Orchard Creek, NY, which drains in series cultivated Histo-
     sols (upstream) and wetlands managed for waterfowl (downstream).  Three
     stations were established, two above and one below the wetland.  Creek-
     water pH averaged about 7.4.  Dissolved concentrations (mg/liter) ranged
     from 0 to 19 for N, 0.009 to 2 for P, 2 to 10 for K, 45 to 411 for Ca, and
     10 to 102 for C, and were highest just below the cultivated areas.  Eighty
     to ninety percent of the creek load was carried from December through
     March.  The cultivated organic soils were a rich source of nutrients to

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     the creek, and the managed wetlands acted as net nutrient sinks for N and
     P during the second year only, when flow through the system was about half
     that in the first year.  However, on an areal basis, much less nutrient
     material was lost from the wetlands than from other parts of the basin.
     The creek system seems to be N-limited; P loads exceeded N loads during
     late summer.  It is suggested that nutrient retention by wetlands depends
     on past nutrient history and current regulation of water movement through
     the system.  (AA)
705. Piecynska, E., V. Sikorska, and T. Ozimek.  1975.  The Influence of
        Domestic Sewage on the Littoral Zone of Lakes.  Polish Archives of
        Hydrobiology (Polskie Archiwum Hydrobiologii) 22:1141-1456.
706. Pilling, J. R., II.  1978.  Design of a Marsh/Pond Sewage Treatment
        System for a 10-unit Housing Development.  Master's Thesis, Department
        of Landscape Architecture and Regional Planning, Univ. of Penn-
        sylvania, Philadelphia.  74 pp.

        This project was entered into under contract with a private developer
     for a 10-unit housing development on a 250 acre site in Buckingham Town-
     ship, PA.  An investigation of three alternative biological sewage treat-
     ment systems, a summary of legal requirements for such a system, a site
     assessment and suitability analysis of the site to accomodate biological
     treatment, and a draft design for a marsh/pond sewage treatment system
     are included in the report.  The marsh/pond system used is an adaptation
     of the technology developed at Brookhaven National Laboratory.  (EP)
707.  Pirrung, D. F., 1978.  Feasibility of Utilization of a Wetland for Nutri-
        ent Removal from Secondary Municipal Wastewater Treatment Plant Efflu-
        ent. Presented at American Water Res. Conf., Wisconsin Section,
        February.

        The objective of this study was to evaluate alternative methods for
     cost effective and environmentally sound treatment of secondarily treated
     effluent from the Waupun City, Wisconsin, wastewater plant.  One of the
     alternatives was to discharge the effluent to Horicon Marsh, a State and
     National Wildlife Refuge, for tertiary treatment.  This alternative,
     based on 30/30 mg/liter effluent limits exhibited an estimated present
     worth cost savings of 1.8 million dollars when compared to conventional
     treatment.  (WE)
708. Pirrung, D.F.  1979.  Feasibility of Utilizing a Wetland for Tertiary
        Treatment.  In; J.C. Sutherland and R.H. Kadlec  (eds.), Wetland Utili-
        zation for Management of Community Wastewater.  Abstracts of a Confer-
        ence held 10-12 July 1979.

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        The City of Waupun,  located  in  east  central Wisconsin,  presently dis-
     charges secondary effluent from its wastewater treatment plant to the
     South Branch of the Rock River.  The  Wisconsin Department  of Natural Re-
     sources has established very  strict effluent  limits  for the discharge of
     the City of Waupun wastewater treatment plant to the Rock  River.   To meet
     the effluent requirements for discharge to  the Rock  River, the City of
     Waupun wastewater treatment plant  would require  upgrading  of the  primary
     and secondary units plus tertiary  treatment.  The requirements for ter-
     tiary treatment are quite stringent and would require very sophisticated,
     energy intensive, unit  processes.
        The City of Waupun 201 Facilities  Plan,  prepared  by Donohue &  Associ-
     ates, Inc., evaluated various alternate discharge points and unit process-
     es in order to obtain the most  cost-effective and environmentally sound
     alternative.  One of the alternatives was to  discharge the secondary
     effluent from the wastewater  treatment  plant  to  a wetland.  The Hoticon
     Marsh, a national wildlife refuge, is located approximately five  miles
     east of the City of Waupun.
        The feasibility of utilizing a  wetland for nutrient removal from
     secondary municipal wastewater  treatment plant effluent consisted of the
     following tasks: a review of  the literature,  site visitations of  similar
     projects in Wisconsin and Michigan, evaluation of the wastewater  charac-
     teristics, and preliminary site investigations.
        The purpose of the wetland alternative was to provide the City of
     Waupun with a less costly simplified  wastewater  treatment  system  which is
     environmentally sound.  The marsh  alternative considered upgrading of the
     primary and secondary treatment units plus  dechlorination, an effluent
     pumping station and a transmission line to  the wetland.  Preliminary costs
     for conventional tertiary treatment were compared to the cost of  discharg-
     ing the secondary effluent to the wetland.  Similar  projects throughout
     the nation, the present state of art, governmental and public input
     regarding the concept are discussed.  (AA)
709. Polin, B. K.  1977.  Re-use of Treated Wastewater:  A Summary Statement.
        Rept. No. 50.  Water Research Foundation of Australia,  Kingsford,
        N.S.W. 60 pp.

        The possible uses of treated or partially-treated sewage for agricul-
     tural, aquacultural or domestic purposes are reviewed, with examples  of
     the current application of such systems in Australia.  Recent experience
     of these methods as a means of conservation of water supplies and re-
     charging of groundwater sources is drawn upon in  order to  assess their
     present and likely future potential in Australia.   It is concluded that
     direct re-use of treated effluent for supplementing drinking water sup-
     plies is inadvisable in the present state of knowledge, and that scarce
     supplies of first-class water should be reserved  for drinking while water
     of poorer quality could be made available for alternative  uses.   Some
     published data regarding water consumption by typical households are
     compared, and details of small-scale treatment facilities  suitable for
     installation in remote situations are outlined.   (AL)
710. Polprasert, C. and P. Edwards. 1981.  Low Cost Waste Recycling  in  the
        Tropics.  Biocycle 22(4):30-35.

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711. Pope, P.R.  1981.  Wastewater Treatment by Rooted Aquatic Plants in Sand
        and Gravel Trenches.  U.S. Environmental Protection Agency.  Municipal
        Environmental Research Laboratory.  Cincinnati, OH.

        A patented process developed by the Max Planck Institute (MPI) of West
     Germany to treat industrial wastes was evaluated as an energy-efficient
     method to treat municipal wastewater.  The major goal was to achieve
     effluents meeting the U.S. Federal Effluent Standards using this novel
     biological treatment process that requires a minimal amount of mechanical
     equipment and manpower for normal operation.
        The Moulton Niguel Water District (MNWD) of Laguna, California, con-
     structed and operated an earthen trench system using rooted aquatic plants
     for the treatment of wastewater.  Two trenches in series were planted with
     the reed Phragmites and the bulrush Scirpus, respectively.
        A 2-month study using conventional secondary effluent as the trench in-
     fluent showed the system was not effective for removing nitrogen and phos-
     phorus components.
        An 11-month study demonstrated that raw screened wastewater applied to
     the trench system at a rate not exceeding 95 m-^/d (25,000 gpd) could be
     treated to secondary effluent quality.  Spatial requirements were about
     the same as for a septic tank system.
712. Porath, D., G. Oron, J. Pollock, and A. Richmond.  1981.  Ammonia Stripping From
        Fish Influent by Duckweed in a Circulating System.  Presented at the Water
        Reuse Symposium II.  Washington, DC.  August 23-28, 1981.

        Free floating duckweed (Lemma gibba) seems to be an excellent ammonia
     stripper in the presence of nitrate, as well as other nutrients and
     wastes found in fish aquaculture.  In standard aseptic conditions, about
     0.1% fresh duckweed biomass was able to strip ammonia (2.0 - 0.2 mg/1) in
     the presence of a higher concentration of nitrate (500 mg/1).  Slight
     changes in pH and light did not significantly affect ammonia uptake.
     About 200 1 water taken from a fish aquaculture which contained 02. mg/1
     ammonia was circulated 5 times a day under the floating duckweed biomass
     in order to improve contact of the plants with the nutrients.  More than
     80% of the ammonia content was stripped after 48 hours.  Ammonia level
     was not changed when the circulating system was activated without the
     duckweed.  (AA)
713. Powers, J. E.  1974.  Critical Variables in Food-item Population Dynamics
        in a Wastewater Aquaculture System, pp. 497-509.  In Wastewater Use  in
        the Production of Food and Fiber-.  U.S. Environmental Protection
        Agency.  Ada, OK.
714. Pratt, D. C., V. Bonnewell, N. J. Andrews, and J. H. Kim.   1980. The
        Potential of Cattail as an Energy Source.  Report prepared for the
        Minnesota Energy Agency, Minneapolis - St. Paul, MN.  147 pp.

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715. Prentki, R. I., T. D. Gustafson,  and M.  S. Adams.   1978.  Nutrient Move-
        ments in Lakeshore Marshes, pp.  169-194.  In R.  E. Good, D. F.
        Whigham, and R. L. Simpson  (eds.), Freshwater Wetlands, Ecological
        Processes and Management Potential.   Academic Press, Inc., New York.

        Seasonal P allocation  in Typha latifolia L. was  investigated by analy-
     sis of above and belowground parts  in a  lakeshore marsh at Lake Mendota,
     Wisconsin.  Maximum total P stocks  of 4.3 and 2.2-2.5 g P/m^ were found
     in summer and winter, respectively.  A maximum upward translocation of 140
     mg P/m^, 1/3 due to mobilization  of belowground reserves, was observed
     the first 2 weeks of June.  Over  an entire summer,  40% of the 3.2g P/m^
     accumulated aboveground was reallocated  from belowground plant parts.
     However, only 23% of this amount  returned to belowground in the fall; 2.5
     g P/m^ was left on the marsh surface as  litter plus leachate.  Typha
     roots continued to absorb P past  aboveground senescence and into early
     winter, resulting in post-shoot-death uptake of up  to 9% of the next
     season's P requirement.
        Analysis of lakeshore marsh literature via a input-output model sug-
     gests that macrophytic translocation of  nutrients is an important source
     of internal loading.  In those marshes without major surface water inputs,
     seasonal accumulation and abandonment of nutrients  above the soild inter-
     face is likely to be the dominant term in nutrient  budgets.  (AA)
716. Price, D.  1975a. The Fate of Fecal Coliforms in Cypress Domes.  In H.T.
        Odum, K. C. Ewel, J. W. Ordway and M. K. Johnson  (eds.), Cypress
        Wetlands for Water Management, Recycling and Conservation, Third
        Annual Report.  Center for Wetlands, Univ. of Florida, Gainesville.
717. Price, D.  1975b.  The Role of Duckweed in Recycling Sewage Effluent in a
        Cypress Swamp, pp. 360-382.  In H. T. Odum, K. C. Ewel, J. W. Ordway,
        and M. K. Johnston (eds.), Cypress Wetlands for Water Management,
        Recycling, and Conservation.  Third Annual Report.  Center for
        Wetlands.  Univ. of Florida.  Gainesville.
718. Price, D. E., and L. Fox.  1973.  Pattern of Distribution of Coliform
        Bacteria in and Around the Experimental Cypress Dome, pp. 285-308.  In
        H. T. Odum, K. C. Ewel, J. W. Ordway, and M. K. Johnston (eds.),
        Cypress Wetlands for Water Management, Recycling, and Conservation.
        Third Annual Report.  Center for Wetlands.  Univ. of Florida.  Gaines-
        ville.
719. Pruder, G.D., J. Carberry and C. Wethe.  1981.  Wastewater-molluscan Aqua-
        culture for Lewes, Delaware.  EPA report (in preparation).

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720. Public Health Service.  1961.  Waste Stabilization Lagoons.  Proceedings
        of a Symposium at Kansas City, MO.  Aug. 1-5, 1960.  PHS, Division of
        Water Supply and Pollution Control.  Washington, DC.  172 pp.

        Contents:  History of raw sewage lagoons in the midwest; History of
     oxidation ponds in the southwest; Lagoon research project of the public
     health service at Fayette, Missouri; Research and installation exper-
     iences in California; Virginia's experimental installation; Sewage
     lagoons in Australia; Economics of waste stabilization lagoons in region
     VI; Use of stabilization ponds in the United States; Missouri basin
     criteria; Lagoon development and acceptance in Mississippi; Experiences
     in Canada; Sewage lagoons and mosquito problems; Lagoon disposal of
     livestock wastes; Operating experiences in the ten Missouri Basin states;
     Arctic sewage lagoons.  (NT)
721. Queen, W.H.  1977. Human Uses of Salt Marshes,  pp. 363-368.  In; Ecosys-
        tems of the World I: Wet Coastal Ecosystems-Elsevier Scientific Pub-
        lishing Co. , New York.

        Present calculations of marsh value emphasize natural characteristics
     and processes revealed by ecological studies over the past several
     decades.  These characteristics and processes may be grouped into four
     categories: biological production, aquaculture, wastewater assimilation,
     and other uses.
        Biologically, estuaries bordered by marshes, and coastal waters in the
     vicinity of extensive marsh-estuarine systems, are among the world's most
     productive waters in terms of both commercial and sport fisheries.  Both
     estuarine shellfish and many coastal water finfish are doubly dependent on
     coastal salt marshes.  Of equal importance with the habitat role is the
     function of marshes as producers of nutrient material, especially organic
     material.  Economic demand has stimulated interest in increasing fish and
     shellfish production in estuarine waters by the use of aquaculture tech-
     niques.  Although these techniques represent commercial exploitation of
     marshes, they are independent upon the production of organic nutrients by
     marsh plants, a natural marsh process.  Wastewater assimilation by marsh
     plants has led to considerable speculation that marshes can be used to
     partially purify domestic wastewater thereby reducing the need for ter-
     tiary sewage treatment.  Other marsh characteristics useful to man are
     erosion control, prevention of damage resulting from tidal flooding, and
     the cycling of nitrogen and sulphur.
722. Rabe, M. L.  1979.  Impact of Wastewater Discharge upon  a Northern
        Michigan Wetland Wildlife Community.  Report to the Houghton  Lake
        Sewer Authority, Houghton Lake, MI.
723. Rai, D.N. and J.D. Munski,  1979.  The Influence of Thick Floating Vegeta-
        tion  (Water Hyacinth: Eichhornia crassipes) on the Physico-chemical
        Environment of a Freshwater Wetland.  Hydrobiologia  62(1):65-69.

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        Physiochemical measurements December 1976-August  1977 of Bhathwa
     Pokhar, a freshwater wetland at Darbhanga, India, showed that  dissolved
     carbon dioxide was higher and that oxygen, pH and temperature  fluctuations
     were lower under water hyacinth areas than in open water.  The extent  of
     difference depends on the time of day and season.  Due to  extreme hyper-
     carbic and hypoxic conditions most vertebrates living in hyacinth infested
     areas are air breathers such as water snakes and turtles or supplemental
     air breathers such as frogs and air-breathing fish.  High  carbon dioxide
     and low oxygen concentrations are due to high anaerobic decomposing mat
     leaves and detritus, increased respiratory activities of organisms, and
     decreased photosynthetic rates caused by water hyacinth shadowing effects
     over the water surface.  Oxygen is very low under water hyacinth mats
     (always less than 2 ppm) and during the summer oxygen levels are reduced
     to zero in both infested and uninfested water areas.  Dissolved oxygen
     reaches its lowest value in late night hours (4 a.m.) and  peaks in the
     afternoon; the maximum-value is 7 ppm in clear areas during winter.
     Bhathwa Pokhar is a shallow (1.5 m mean depth), small (1.5 ha), swampy
     pond and 98% area is covered by water hyacinth.  The source of pond water
     is Darbhnage Medical College campus sewage.
724.  Ramsay, A.  1976.  Comparison of Fall Birds Present in a Natural Cypress
        Dome, a Sewage Dome, and the Adjacent Pine Plantation, pp.  654-
        In H. T. Odum, K. C. Ewel, J. W. Ordway, and M. K. Johnston (eds.),
        Cypress Wetlands for Water Management, Recycling, and Conservation.
        Third Annual Report.  Center for Wetlands.  Univ. of Florida.  Gaines-
        ville.
725. Ramsay, A.  1978.  The Effect of the Addition of Sewage Effluent  on
        Cypress Dome Bird Communities.  Master's paper.  School  of Forest
        Resources and Conservation, Univ. of Florida, Gainesville. 22  pp.
726.  Randall, C.  W., and L. D. Benefield.  1980.  Biological Process Design
        for Wastewater Treatment.  Prentice-Hall, Inc.  Englewood Cliffs, NJ.
        540 pp.

        This volume is a practical treatise on the design of biological  ef-
     fluent treatment plant which bases the calculations employed on an
     understanding of the theoretical principles involved.  The  first  two
     chapters are concerned with the fundamentals of process kinetics  and
     microbiology (including growth kinetics) respectively, and  are followed
     by a section on wastewater characteristics and flows.  Thereafter,  a
     series of chapters on specific treatment applications is presented, in-
     cluding activated sludge and its various modifications, aeration  system
     design, treatment ponds and aerated lagoons; attached-growth biological
     treatment methods, and sludge digestion.  The problems and  processes of
     nitrification and denitrification are considered where appropriate, in
     addition to the oxidation of organic matter.  (AL)

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 727.  Raschke,  R.  L.   1968.   Algal Periodicity,  Primary Production and Waste
         Reclamation  in  a Tertiary Sewage Stabilization Pond Ecosystem.  Ph.D.
         Diss.   Iowa  State Univ.,  Ames.
 728.  Reddy,  C.  N.,  and W.  H.  Patrick,  Jr.   1977.   Effect of Redox Potential
         and  pH  on the Uptake  of Cadmium and Lead by Rice; Plants.  J. Environ.
         Qual. 6:  259-262.

         Redox potential and pH are two of  the major factors influencing the
      mobilization and immobilization of heavy metals in flooded soils and sedi-
      ments,  and their availability to plants.  A system developed for growing
      plants  in  soil suspensions where redox potential and pH can be controlled
      was used to study the uptake of cadmium (Cd) and lead (Pb) by rice plants
      (Oryza  sativa L.).  Uptake of Cd and Pb by root and shoot tissue, and their
      translocation from root  to shoot, was determined at six different redox
      potentials (-200, -100,  0, +100,  +200, and +400 mV) and four pH values
      (5,6,7, and 8).   The  effects of redox potential and pH on the levels of
      water-soluble Cd and  Pb  in the soil suspensions were also studied.  Almost
      all Cd  entering the rice plants accumulated in the shoots.  Total Cd
      uptake  and shoot uptake  increased with an increase in suspension redox
      potential  and a decrease in pH.  Water-soluble Cd in the soil suspension
      was significantly correlated with total plant Cd and Cd uptake by shoot.
      Total Pb uptake,  including Pb associated with the roots, decreased with an
      increase in pH.   No definite redox potential effect was found on Pb uptake
      by the  shoot.   Lead was  less mobile than Cd in the rice plants and was
      primarily  associated  with the roots.   Water-soluble Pb decreased with an
      increase in redox potential and pH, and was significantly correlated with
      total plant Pb uptake and Pb uptake by shoot.
 729.  Reddy,  K.  R.,  and D.  A.  Graetz.   1980.   Use of Shallow Reservoirs and
         Flooded Soil Systems  for Agricultural Drainage Water Treatment: In-
         organic nitrogen removal.  J.  Environ. Qual. (in review).
 730.  Reed,  C.A.,  and T.  Kubiak.   In press.   An Ecological Evaluation Procedure
         for Determining Wetlands Suitability for Wastewater Discharge/Treat-
         ment.   In;  Ecological Considerations in Wetlands Treatment of Municipal
         Wastewaters, Proceedings of a Workshop, 23-25 June 1982. University of
         Massachusetts,  Amherst MA.  US Fish and Wildlife Service and US
         Environmental Protection Agency.


 731.  Reed,  S.  C.,  and R. K.  Bastian (eds.). 1980.  Aquaculture Systems for
         Wastewater Treatment: An Engineering Assessment.  EPA-430/9-80-007.
         U.S.  Environmental Protection Agency, Office of Water Program
         Operations, Municipal Construction Division.  Washington, DC.  127 pp.

         This publication contains five additional engineering assessments not
      included in Bastian and Reed (1979).
1088.  Reed,  S^C.  and R.K.  Bastian.   In press.

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732. Reed, S. C., R. K. Bastian, and W. Jewell.   1979.  Engineering Assessment
        of Aquaculture Systems for Wastewater Treatment: an Overview,
        pp. 1-12.  In R. K. Bastian and S. C. Reed  (eds.), Aquaculture
        Systems for Wastewater Treatment: Seminar Proceedings and Engineering
        Assessment.  EPA 430/9-80-006.  U.S. Environmental Protection Agency,
        Office of Water Program Operations, Municipal Construction Division,
        Washington, DC.

        This overview contains a summary of the current status of aquaculture
     technologies, including a section on wetland treatment processes.  For
     the purposes of this assessment, wetland processes were defined as those
     treatment processes that take place on land where the water table is at
     or above the surface for long enough each year to maintain saturated soil
     conditions and the growth of related vegetation.  A number of conclusions
     and recommendations for further studies are  listed for wetland processes
     and for the other sections of this publication (e.g., aquatic plant
     systems and combined systems). (EP)
733.  Reid, G. W.  1976.  Algae Removal by Fish Production.  In E. F. Gloyna,
        J. F. Malina, Jr., E. M. Davis (eds.J, Ponds as a Wastewater Treatment
        Alternative.  Water Resources Symp. #9, Center for Research in Water
        Resources, College of Engineering, Univ. of Texas, Austin.
734.  Reim, J. 1980.  Sewage Treatment in a Sphagnum Peat Bog.  Great Lakes
        Focus on Water Quality 6(3):6-9.
735.  Reimold,  R.J.,  1980.  Marsh Creation: Impact of Pesticides on the Fauna,
        Use of Infrared Photography, Ditching and Diking,  pp. 132-135.  In;
        Rehabilitation and Creation of Selected Coastal Habitats: Proceedings
        of a Workshop, Sapelo Island, Georgia, 16-20. May, 1976.  Fish and
        Wildlife Service, Biological Services Program, Washington, DC Report
        FWS/OBS-80/27.  November.

        Species diversity is used as an indication of the effects of toxaphene
     on the fauna of two estuarine systems.  Brunswick Estuary in Georgia
     receives  toxaphene from a manufacturing plant and from agricultural run-
     off.   The Duplin Estuary is a pristine estuary, part of a National
     Estuarine Sanctuary.  The study covers the period 1968-1976.  Except for
     the early years of the study (1968-1971), no mesurable quantities of
     toxaphene were  found anywhere except very close to the manufacturing
     plant.  Since the study began,  the levels of toxaphene in the manufactur-
     ing plant effluent decreased from parts per hundred to parts per billion.
     There was a dramtic change both in the number of species and in the num-
     ber of individuals over the years.  The diversity indices for Terry Creek,
     the stream into which the toxaphene effluent was discharged, revealed that
     in 1968 there were only three different kinds of organisms, as opposed to
     nearly 25 species by 1976.  Biomass also increased with time.  After the
     first three years, the number of species remain more constant in the
     Brunswick Estuary than it did in the natural Duplin Estuary.  Color infra-

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     red aerial photography is useful for ecological delineation and for econ-
     omic delineation including legal purposes.  Ditching and diking for habi-
     tat creation and mosquito control have a variety of effects on marshes.
     Ditching reduces the water level, can cause invasion of upland species,
     and reduces the area of vegetated wetland.  Another problem with ditching
     is the creation of cat clays.  The various consequences of alteration of
     the salt marsh ecosystem should be evaluated when ditching or diking is
     being considered.
736. Reppert, R.T.  W. Sigleo, E. Stakhiv, L. Messman, and C. Meyers.  1979.
        Wetland Values: Concepts and Methods for Wetlands Evaluations. Research
        Kept. 79-41. U.S. Army Corps of Engineers Institute for Water
        Resources, Fort Belvoir, VA.  109 pp.

        Report presents concepts and methods for the evaluation of wetlands
     pursuant to Section 404, PL 92-500 (as amended), President Carter's May
     24, 1977 Executive Order on wetlands protection, and other statutory and
     Administrative authorities.  The evaluation of wetlands is based on the
     analysis of their physical, biological and human use characteristics.  The
     report discusses these functional characteristics and identifies specific
     criteria for determining the efficiency with which the respective func-
     tions are performed.
        Two potential wetlands evaluation methods are described.  One is a non-
     quantitative method in which individual wetland areas are evaluated based
     on the deductive analysis of their individual functional characteristics.
     The other is a semi-quantitative method in which the relative values of
     two or more site alternatives are established through the mathematical
     rating and summation of their functional relationships.
        The specific functions and values of wetlands which are covered in this
     report are (1) natural biological functions, including good chain produc-
     tivity and habitat, (2) their use as sanctuaries, refuges or scientific
     study areas, (3) shoreline protection, (4) groundwater recharge, (5) stor-
     age for flood and storm water, (6) water quality improvement, (7) hydro-
     logic support, and (8) various cultural values.
737. Reynolds, B.J. 1976.  An Example of Sewage Disposal by Spray Irrigation,
        pp. 218-220.  In; G. Berg, H.L. Bodily, E.H. Lennette, J.L. Melnick,
        and T.G. Metcalf (eds.), Viruses in Water.  American Public Health
        Association, Inc., Washington, D.C.
738. Reynolds, J.H.  1981.  Land Application of Municipal Wastewater.  J. Water
        Pollut. Control Fed.  53(6):767-769.
739. Rich, L.G. 1980.  Low Maintenance Mechanically Simple Wastewater Treat-
        ment Systems.  McGraw-Hill Book Co., New York.  219 pp.

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         The basic principles and mechanisms for biological treatment of sewage
      are summarized and a number of low maintenance, mechanically simple
      systems,  which can reliably treat sewage flows of 1 mgd or less, are
      described.   They comprise activated-sludge systems, lagoon systmes, solids
      removal and stabilization systems, and fixed-film systems.  In addition,
      land disposal of both sewage and sewage sludge is discussed.  The design
      equations and criteria governing the performance of the varioun systems
      are presented as a basis for practical operation and control of the
      systems in question, with particular emphasis on the fate of the suspended
      solids and the maintenance of acceptable suspended solids concentrations
      in the final effluent.  (AL)
    .  Richardson,  B.   1981.
 740.  Richardson,  C.J.  1976.  Plant Growth, Nutrient Accumulation and Decompo-
         sition in a Central Michigan Peatland Used for Effluent Treatment.
         Proc.  of  Symposium on Freshwater Wetlands and Sewage Effluent Disposal.
         Univ.  of  Michigan, Ann Arbor.
1089.  Richardson, C.J.  and D.S. Nichols.  In press.
 741.  Richardson,  C.  J., J.  A. Kadlec, W. A. Wentz, J. P. M. Chamie, and R. H.
         Kadlec.   1975.  Background Ecology and the Effects of Nutrient
         Additions on a Central Michigan Wetland.  Publ. No. 4.  Univ. of
         Michigan, Ann Arbor. 64 pp.

         In central Michigan, studies are currently underway to determine the
      effects of  nutrient additions on the structure and function of a wetland
      (peatland)  ecosystem.   Field experiments were conducted to determine the
      effects of  nutrient additions on various components of wetland eco-
      systems. A simulation model was utilized.  The underlying purposes of
      these studies were to test the feasibility of wetlands to remove
      nutrients from  secondarily treated sewage wastewater and to determine the
      magnitude of any changes in the structure and function of the wetland
      ecosystem.   Parameters discussed in this paper are net primary produc-
      tion, nutrient  uptake and transfers, water quality, and decomposition
      rates.  (NT)
 742.  Richardson,  C.J.,  D.L. Tilton, J.A. Kadlec, J.P. Chamie, and W.A. Wenz.
         1978.   Nutrient Dynamics in Northern Wetland Ecosystems, pp. 217-241.
         In;  R.E.  Good,  D.F. Whigham, and R.L. Simpson (eds.), Freshwater Wet-
         lands:  Ecological Processes and Management Potential.  Academic Press,
         Inc.,  New York.

         Nutrient  information was reviewed by compartment (i.e. soils, plants,
      water)  and at the  ecosystem lever among 4 northern wetland types: fens,
      bogs,  swamps, and  marshes.  Total soil N, P, and Ca were lowest in bog
      peats  but the Mg content did not vary appreciably among wetland types.
      Cation exchange capacity was >100 meq/100 g for all wetlands.  Concentra-
      tions  of  N,  P,  Ca  and Mg were significantly lower in bog plants than fen
      plants.   Seasonal  patterns of nutrient concentrations (N and P) in leaves
      and steins decreased through the growing season in fen plants.  Transloca-
      tion of nutrients  from plant parts prior to abscission did not occur in

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     the plants studied.  Minerotrophic fen water chemistry differed  from  that
     of ombrotrophic bogs in that fen waters were dominated by Ca++ and HCO3~
     ions and bog waters were dominated by H4 and SC>4++ ions  (Moore and Bell-
     amy, 1974).  Seasonal variations in NH4-N, NC^-N and T?O^P were closely  re-
     lated to peatland hydrology, organism uptake and peat exchange character-
     istics.  A cycling study for a central Michigan fen revealed that >97%  of
     the N, P and Ca was in the peat compartment. The turnover time for N  in
     aboveground biomass was  2 years.  An examination of plant uptake rates of
     N and P in the Michigan fen revealed that low plant productivity may  be
     related to low N and P availability.  Nutrient outputs for wetland ecosys-
     tems, when compared to yields from terrestrial forest systems, indicate
     that natural outputs for some wetland types are well within the  range,  or
     in some cases exceed, outputs from upland terrestrial ecosystems.  The
     capacity of acid peatlands to store or assimilate additional P or K on  a
     long-term basis appears limited.  Nutrient dynamics in wetlands  at the
     organism, community and ecoystem level are all poorly understood and  are
     deserving of further study.  (AA)
743. Richardson, C. J., W. A. Wentz, J. P. M. Chamie, J. A. Kadlec, and D. L.
        Tilton.  1976.  Plant Growth, Nutrient Accumulation and Decomposition
        in a Central Michigan Peatland Used for Effluent Treatment, pp.
        77-118.  In D. L. Tilton, R. H. Kadlec, and C. J. Richardson  (eds.),
        Proceedings of A National Symposium on Freshwater Wetlands and Sewage
        Effluent Disposal.  Univ. of Michigan, Ann Arbor.

        Studies were performed on the impacts of artificial nutrient  loading
     on peatland vegetation in Michigan.  Experiment plots were treated with
     two, four, and ten times the normal levels of nutrient salts.  Short  term
     results indicate that nutrient addition results in the enhancement of
     growth of some species, tissue concentrations are increased, and litter
     and organic soils function as a nutrient sink.  Specifically, sedge N and
     P levels and sedge biomass increased over controls in the 4x and 10x
     plots, but that growth of grass and aster decreased.  No changes in
     litter decomposition rate were noted in any treatment plots, although
     nutrient levels of plant tissue were significantly higher in the plots
     that received the lower-level applications (only P was significantly
     higher in the 4x and 10x plots).  Nutrient processing in this system was
     accomplished by denitrification in soil, sorption by litter and  peat
     soil, and nutrient uptake by plants.  The authors conclude that  such
     natural filtering systems offer a short-term solution for removing a
     limited amount of nutrient input. (EP)
744. Rickards, W. L.  1973.  The Feasibility of Penaeid Shrimp Culture  in
        Brackish Ponds Receiving Treated Sewage Effluent.  Sea Grant Reprint
        No. 58.  North Carolina State Univ. Raleigh, NC. 10 pp.

        The report focuses on a study conducted as an extension of a previous
     project where penaeid shrimp had been stocked in control and treated  sew-
     age ponds — shrimp grew in control ponds but failed to survive in treated
     sewage ponds.  It has been concluded in the earlier project that low
     levels of dissolved oxygen in the water at night and wide diurnal  pH  flue-

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     tuations were responsible for shrimp failure.  The subsequent project was
     designed to modify oxygen and pH regimes in a sewage pond and subsequently
     stock it with shrimp to determine whether or not factors in addition to
     oxygen and/or pH has been responsible for the previous shrimp mortalities.
     It was found that shrimp were able to survive and grow in the aerated
     sewage pond.  (NT)
745. Robbins, R.,  and D. Lagerroos (eds.).  1981.  Materials on Floodplains
        and Wetlands: Legal Restraints and Options.  American Bar Association
        Special Committee on Housing and Development Law.  Prepared for a
        workshop at Chicago, IL, 28-29 January 1981.
746. Roberts, M. H.  1980.  Detoxification of Chlorinated Sewage Effluent by
        Dechlorination in Estuarine Waters.  Estuaries 3(3):184-191.

        Secondary treated sewage was chlorinated to a residual level of 2-10
     mg/1 in a continuous flow system in the laboratory and  diluted to various
     levels with estuarine water.  A portion of the chlorinated sewage
     effluent was dechlorinated with sodium thiosulfate and  the dechlorinated
     waste was diluted with estuarine water in the same proportions as the
     chlorinated waste.  Menidia menidia, Palaemonetes pugio and Crassostrea
     virginica were exposed to chlorinated and dechlorinated treatments.  High
     concentrations of chlorinated effluent caused mortalities in M. menidia
     and P. pugio.  Similarly, the chlorinated wastes depressed the shell
     deposition rate of C. virginica below 50% of controls at all doses.   (PA)
747. Robinson, A. C. et al.  1976.  An Analysis of the Market Potential  of
        Water Hyacinth-Based Systems for Municipal Wastewater Treatment.
        Battelle Research Rept. BLC-OA-TFR-76-5, Battelle Columbus Lab.,
        Columbus, OH.
748. Roche, W. M., 1973.  Wastewater Reclamation and Reuse in the Suisun Marsh,
        California,  pp. 326-335.  In; Water for the Human Environment, Vol.
        Ill, Tech. Sessions, Proceedings of the First World Congress  on Water
        Resources. Chicago.  September 24-28.

        The U.S. Bureau of Reclamation, Solano Irrigation District, and  .ity of
     Fairfield are conducting a wastewater reclamation and reuse pilot   jmon-
     stration program in the Suisun Marsh, Solano County, California.   he  pro-
     gram objective is to demonstrate the compatibility of beneficial i ;use and
     treatment of municipal and industrial wastewater with marsh manage nent.
     The program is designed to provide information to assist the loca  area in
     meeting its waste treatment and disposal obligations.  To provide informa-
     tion on the reuse of wastewater for agricultural purposes, and tc provide
     the Bureau of Reclamation with information on a source of water  for meet-
     ing its responsibility for protection of the Suisun Marsh.  The program

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      evaluates several features which beneficially use wastewater and also
      remove  nitrogen.   Field facilities include a detention reservoir, a
      sprinkler-irrigated and tile-drained pasture, marsh ponds, and irrigated
      marshland.   The facilities are designed to remove 90% of the nitrogen from
      the wastewater that eventually flows to the marsh channels.
 749.  Rodgers,  E.  W.   1976.   Everything is Ducky at Vandenberg AFB.  Water and
         Wastes Engineering 13(10):43-44.
 750.  Roels,  O.  A.,  V.  Harris,  V.  Lee,  and B.  Sharfstein.  1977.  Effect of
         Detergent Legislation  on Phosphorus in Effluent and Receiving Waters.
         J. Water Poll.  Control Fed.  49(9):2017-2021.

         A small effluent aquaculture system has been operated at the Tallman
      Island  sewage  works of New York City since 1972,  to remove dissolved
      inorganic  N and P from secondary sewage effluent before discharge to the
      East river. Regular monitoring has shown a marked increase in the molar
      N:P culture phytoplankton in this system, and studies have confirmed that
      this is due to recent state legislation banning the use of P in deter-
      gents.   This has resulted in a reduction of more than 50% in the pro-
      duction of protein in the aquaculture system.  (AL)
 751.  Rogers,  H.  H.,  and D.  E.  Davis.   1972.  Nutrient Removal by Water
         Hyacinth.  Weed Sci.  20:423-428.
 752.  Rohlich,  G.  A.,  and D.  P. Uttermark.  1972.  Wastewater Treatment and
         Eutrophication,  pp.  231-245.   In G.  E.  Likens (ed.), Nutrients and
         Eutrophication.   Amer. Society of Limnol. Oceanogr., Special Symposia,
         Vol.  1.


 753.  Rosman, L.   1978.   Impact Assessment of a Northern Michigan Wetland
         Invertebrate  and Vertebrate Fauna Receiving Secondarily Treated Sewage
         Effluent.
 754.  Rudolfs,  W.,  L. L.  Frank, and R. A. Ragotzkie.  1950.  Literature Review
         on the Occurrence and Survival of Enteric, Pathogenic, and Related
         Organisms  in Soil, Water, Sewage, and Sludges, and on Vegetation.
         Sewage &  Industrial Wastes 22:1261-1281.
1090.  Rusincovitch, F.   In press.


 755.  Russell, W.C. et al.  1981.  Detergents and Waterfowl. J. of Zoo Animal
         Sci.  12(1):10-12.

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756. Rybczynski, W., C. Polprasert, and M. McGarry.   1978.  Low Cost Tech-
        nology Options for Sanitation: A State of the Art Review and Annotated
        Bibliography.  International Development Research Center and World
        Bank Report IDRC-102E.

        Technologies available for primitive and advanced sewage treatment
     systems are reviewed.  Alternative approaches to collection, treatment,
     reuse, and disposal of human wastes are presented as an aid to
     policy-makers and technicians faced with instituting or improving  sewage
     programs.  Options for waste disposal in hot climates also are discussed.
     Included is an annotated bibliography of literature on sewage treatment
     and handling.  The bibliography contains sections on: deposition devices;
     onsite collection and treatment-pit latrines, composting privy, septic
     tank and aqua-privy; collection and offsite treatment-cartage, water-
     borne, ponds, composting, aquatic weeds; reuse-irrigation, aquaculture,
     algae, fertilization, biogas; greywater; and water conservation.   (EL)
757. Ryther, J. H.  1973.  The Use of Flowing Biological Systems in Aquacul-
        ture, Sewage Treatment, Pollution Assay, and Food-Chain Studies.
        Tech. Rept. 73-2.  Woods Hole Oceanographic Institution, Woods Hole,
        MA.  506 pp.

        A combined tertiary sewage treatment-marine aquaculture system has
     been designed and successfully tested on a small, experimental scale.  In
     this system, the effluent from secondary sewage treatment, diluted with
     seawater, is used as a source of nutrients for the growth of unicellular
     marine algae, and the algae, in turn, are fed to oysters or other shell-
     fish.  The algae remove the objectionable constituents from the  secondary
     sewage effluent (ammonia, nitrate, phosphate, etc.) and the algae are
     removed by the oysters.  The products are purified waste effluent, which
     will not support further algal growth (undesirable "algae blooms") in
     nature, and a commercially-valuable crop of seafood.  Using a continuous
     flow mode of operation, the process was capable of removing 95-100% of
     the inorganic nitrogen content of the sewage effluent, the discharge from
     the system being unable to support further algal growth and often con-
     taining less nitrogen than the receiving seawater.  (NT)
758. Ryther, J. H.  1974.  The Use of Flowing Biological Systems  in Aqua-
        culture, Sewage Treatment, Pollution Assay, and Food Chain Studies.
        Rept. to National Science Foundation.  Woods Hole Oceanographic
        Institution.  Woods Hole, MA.  371 pp.

        The study is concerned with the growth and nutrient-uptake kinetics  of
     several species of marine phytoplankton.  These were studied by means of
     chemostat culture techniques in an attempt to determine the  optimal com-
     bination of sewage effluent and seawater in the medium, algal yield, and
     nutrient utilization consistent with the concept of combined ter-
     tiary-treatment aquaculture system.  Output from the continuous algal
     cultures was fed to juvenile oysters in an attempt to determine growth  of
     the molluscs as a function of algal food species.  Other, separate
     experiments were conducted to determine the effect of concentration of  a

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     single diatom species, Phaedactylum tricornutum, on the rate and effi-
     ciency of food assimilation deposition  (as feces and pseudofeces) and
     growth of juvenile oysters.  These and  other aspects of the study are
     fully detailed.  (NT)

759. Ryther, J. H.  1975a. Investigative Research and Management of Effluent
        from Secondary Wastewater Treatment  Utilizing a Marine Aquaculture
        System for Removal of Inorganic Nutrients.  Final Report to National
        Science Foundation.  Woods Hole Oceanographic Institution, Woods Hole,
        MA.  60 pp.

        A combined waste recycling-marine aquaculture system capable of com-
     plete N removal from treated domestic wastewater has been developed and
     tested on a pilot-plant scale over a 1  year period.  Effluent from
     secondary sewage treatment, mixed with  seawater, is used to grow unicell-
     ular marine algae in large, continuous-flow, outdoor mass cultures.
     Harvest from the algae cultures is fed  to oysters and other filter-feed-
     ing bivalve molluscs and to secondary crops of flounders or lobsters.
     Dissolved wastes produced by the animals are assimilated by cultures of
     commercially-valuable seaweeds.  Successful cultures of unicellular
     algae, mostly diatoms, and seaweeds have been sustained over long periods
     of time (months) with only minor problems.  Bivalve mollusc culture was
     unsuccessful during the first year of operation.  An alternative
     N-removal system consisting only of seaweeds fed a continuous flow of
     secondary sewage effluent mixed with seawater has also been evaluated.
     Year-round operation would be possible  in tropical to semi-tropical
     climates.  In temperate climates, operation of the system is possible
     only on a seasonal basis.  (NT)
760. Ryther, J. H. 1975b.  Physical Models of Integrated Waste Recycling-marine
        Polyculture Systems.  Aquaculture 5:163-177.
761. Ryther, J. H.  1975c. Preliminary Results with a Pilot Plant Waste
        Recycling Marine-Aquaculture System.  Tech. Rept. No. 75-41.  Woods
        Hole Oceanograhic Institution.  Woods Hole, MA.  61 pp.

        A combined waste recycling-marine aquaculture system capable of com-
     plete nitrogen removal from treated domestic wastewater has been
     developed and tested on a pilot-plant scale over a one-year period.
        Effluent from secondary sewage treatment, mixed with seawater, is used
     to grow unicellular marine algae in large, continuous-flow, outdoor mass
     cultures.  Harvest from the algae cultures is fed to oysters and other
     filter-feeding bivalve molluscs and to secondary crops of flounders or
     lobsters.  Dissolved wastes produced by the animals are assimilated by
     cultures of commercially-valuable seaweeds.  Successful cultures of uni-
     cellular algae, mostly diatoms, and seaweeds have been sustained over long
     periods of time (months) with only minor problems.  Bivalve mollusc cul-
     ture was unsuccessful during the first year of operation.  An alternative
     N-removal system consisting only of seaweeds fed a continuous flow of
     secondary sewage effluent mixed with seawater has also been evaluated.
     Year-round operation would be possible in tropical to semi-tropical

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     climates.  In temperate climates, operation of the system is possible
     only on a seasonal basis.  (NT)
762. Ryther, J. H.  1976a. Preliminary Results with a Pilot Plant Waste
        Recycling Marine Aquaculture System.  Report to the National Science
        Foundation.  Woods Hole Oceanographic Institution.  Woods Hole, MA.
        46 pp.

        A biological, tertiary sewage treatment-marine aguaculture system has
     been developed, tested, and evaluated over one year on a "pilot-plant"
     scale at the Environmental Systems Laboratory (ESL) of the Woods Hole
     Oceanographic Institution.  The effluent from secondary sewage treatment,
     mixed with seawater, is used as a source of nutrients to grow sin-
     gle-celled marine algae in mass, continuous flow-through cultures.  Algal
     cultures diluted with seawater are fed into cement raceways containing
     stacked trays of shellfish.  The trays were stocked with the American
     oyster (Crassostrea. Virginia) and the hard clam (Mercenaria mercenaria)
     as well as smaller numbers of other shellfish species.  The phytoplankton
     remove the nutrients from sewage effluent which has varied experimentally
     from 10-50% of the effluent-seawater mixture.  The shellfish remove the
     phytoplankton from the water.  Prior to its discharge, the effluent from
     the shellfish cultures is passed through seaweeds grown in suspended
     culture to remove nutrients not initially assimilated by phytoplankton
     and excretion from the shellfish.  (NT)
763. Ryther, J. H.  1976b. Marine Polyculture Based on Natural Food Chains and
        Recycled Wastes.  Tech. Rept. 76-92.  Woods Hole Oceanographic Insti-
        tution. Woods Hole, MA.

        Research has continued during the past Sea Grant fiscal year on the
     development, testing, and evaluation of a combined waste recycling-marine
     polyculture system.  The concept of the system is to grow unicellular
     marine algae (phytoplankton) in mixtures of seawater and the effluent
     from a secondary sewage treatment plant.  The algae grown in continuous
     flow-through cultures, are then fed to bivalve molluscs, such as oysters,
     clams, scallops, or mussels.  The algae remove the nutrients from the
     wastewater and the molluscs remove the algae.  Finfish  (winter flounder)
     and Crustacea (American Lobster) are stocked as post-larval or juvenile
     animals together with the molluscs to feed upon the solid wastes (feces
     and pseudo feces) produced by the shellfish and upon the small inverte-
     brate fauna (polychaete worms, amphipods, etc.) that are supported by
     these wastes.  Seaweeds constitute a final polishing stage to the system,
     receiving the effluent from the animal culture system and removing from
     it nutrients regenerated through excretion and metabolism of the animals
     as well as any nutrients from the wastewater not initially removed by the
     phytoplankton.  Twelve reports by various authors are compiled in this
     volume*  (NT)

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764. Ryther, J. H.  1979.  Treated Sewage Effluent as a Nutrient Source for
        Marine Polyculture, pp. 351-375.  In R. K. Bastian and S. C. Reed
        (eds.), Aquaculture Systems for Wastewater Treatment: Seminar Pro-
        ceedings and Engineering Assessment.  EPA 430 9-80-006.  U.S. Envir-
        onmental Protection Agency, Office of Water Program Operations,
        Municipal Construction Division.  Washington, DC.

        A biological tertiary sewage treatment-marine aquaculture system was
     developed, tested, and evaluated for two years on a "pilot-plant" scale at
     the Woods Hole Oceanographic Institution's Environment Systems Laboratory.
     The effluent from secondary sewage treatment, mixed with seawater, was
     used as a source of nutrients to grow single-celled marine algae (phyto-
     plankton) in mass (35,000 gallon), continuous flow-through cultures.  Har-
     vest from the algal cultures (experimentally varied from 25% to 75% of the
     culture volume/day), diluted with seawater, was fed into 40' x 4' x 5'
     (deep) cement raceways containing stacked trays of shellfish.  The latter,
     stocked at densities ranging from 75,000 to 150,000 animals/raceway (1,500
     - 3,000 per tray) have consisted of different species of oysters and
     clams, and smaller numbers of other shellfish.
        The phytoplankton removed the nutrients from the sewage effluent, which
     varied experimentally from 10 percent to 50 percent in the effluent-sea-
     water mixture.  The shellfish removed the phytoplankton from the water.
     Effluent from the shellfish cultures (i.e., the pond harvest and diluting
     seawater) prior to its discharge was passed through a culture of seaweeds,
     grown in suspended culture in raceways adjacent to the shellfish cultures,
     which serve as a final polishing step, removing nutrients not initially
     assimilated by the phytoplankton and those regenerated by excretion of the
     shellfish and decomposition of their solid wastes.  After initial experi-
     mentation with several seaweed species, research was concentrated on two
     red algae of potential commercial value, Gracilaria tikyahiae and Neoag-
     ardhiella baileyi. (which contain the polysaccharides, agar and carrageenan
     respectively).  (AA)
765. Ryther, J.H. et al.  1972.  Controlled Eutrophication-increasing Food Pro-
        duction from the Sea by Recycling Human Wastes.  BioScience 22:144-152.
766. Ryther, J.H., T.A. DeBusk, M.D. Hanisak, and L.D. Williams. 1979.  Fresh
        Water Macrophytes for Energy and Wastewater Treatment, pp. 652-660. In;
        P.E. Greeson, J.R. Clark, and J.E. Clard (eds.), Wetland Functions and
        Values:  The State of Our Understanding, Amer. Water Resou. Assoc.
        Tech. Publ. TPS 79-2.  Minneapolis, NM.

        Fresh water weed crops are capable of simultaneously providing advanced
     treatment (nutrient removal) of wastewater passed through them and produc-
     biomass that may be converted to fuel by anaerobic digestion.  Performance
     of both functions depends upon the rate of primary organic production of
     the plant species in question.  Annual yields of the floating species,
     Lemna minor (duckweed) and Eichhornia. crassipes (water hyacinth), and of
     the submerged species, Hvdrilla vertic illata, were determined in central
     Florida.  Yields of duckweek and Hydrilla, 13.5 and 15.3 m tons/ha/year,
     respectively, are comparable to many temperate terrestrial food and  grass

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     crops.  The yield of water hyacinths, 88.3 m  tons/ha/year,  is  among the
     highest documented for any plant  species on earth.  A  one thousand  hectare
     water hyacinth farm is capable of producing tO^BTU of energy  per
     year as methane gas and, at the same time, of  removing the  nutrients from
     the wastewater of a population of 700,000 people.  (AA)
767. Salmon, J. E.  1973.  A Question as to the Possibility of Utilizing
        Plants Capable of Assimilating Heavy Metals  to Practical Ends.  Biol.
        J. Linn. Soc. Abstract.  5:357.
768. Sanders, J.G. and E.J. Kuenzler, 1979.  Phytoplankton Population Dynamics
        and Productivity in a Sewage-Enriched Tidal Creek in North Carolina.
        Estuaries 2:87-96.

        Calico Creek differs from neighboring estuaries  in that  it receives
     sewage effluent, and its waters therefore contain ample nutrients. High
     nutrient levels enable the phytoplankton population, which  is probably
     light-limited, to reach densities of a thousand billion cells/1 during -the
     summer, 2 to 3 orders of magnitude higher than the  surrounding, nutrient-
     limited populations.  At cell densities greater than 10 million cells/1
     the number of dominant species is greatly reduced and the level of diver-
     sity drops sharply.  Calico Creek also differs by being very shallow; the
     population can be drastically affected by high runoff.  The productivity
     of both the phytoplankton and the surrounding Spartina marsh is much high-
     er than the neighboring unpolluted estuaries.  Unlike the larger and more
     stable estuaries nearby, the dependence of the population on the effluent
     for nutrients, the possibility of toxic materials entering the creek with
     the effluent, and its small size make Calico Creek  subject to sudden
     change.
769. Sanville, W. D.  1979.  Productivity Response of an Alaskan USA, wetland
        Plant Community to Nutrient Enrichment.  Proceedings of the Alaska
        Science Conference 30:137.  U. S. Environmental Protection Agency,
        Corvallis Environmental Research Laboratory. Corvallis, OR.
770. Sauze, F.  1975.  Algae, a Resource for the Future?  From Water Purifica-
        tion...to Aquaculture and Energy Production.  Tech. Sci. Munic.
        (France) 70(12): 537-540.

        The role of phytoplankton in solving problems of pollution, protein
     shortage, and energy replacement is discussed with reference to research
     throughout the world.  Purification of sewage-works effluents by algae in
     lagoons could produce quantities of plankton, small crustaceans, and
     molluscs for use as food for higher animals, or as fertilizer.  Marine
     farming presents economic problems but a simpler type of aquaculture,
     similar to purification lagoons, is visualized for production of carp,
     eels, or trout.  Rotation of crops, such as fodder, rice, and fish, is

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     possible on drainable ponds.  Energy production from cellulosic wastes,
     from the sun via plankton, and by "biophotolysis" of water is also
     reviewed.  (EL)
771. Savannah River Ecology Lab, 1972.  Distribution and Cycling of Radioactive
        Isotopes Released into the Savannah River Swamp Forest:  Environmental
        Implications Progress Report.  Nov. 1, 1971 to Aug. 1, 1972.  Aiken,
        South Carolina.  NTIS No. SRO-708-1.

        Reports progress of a study on the environmental implications of  radio-
     activity released to a Savannah River swamp forest.  The specific objec-
     tives were to - (1) completely review the literature pertinent to the pro-
     posed studies and study areas.  (2) Define and mark off the limits of the
     study area and sampling areas within the overall plot.  (3) Compile  a list
     of the most abundant or dominant species in the area and determine the
     degree to which the various species differ in the concentrations of  the
     radioactive isotopes: CS137, CO60, ZN56.  (4) Identify the potential prob-
     lem species that, because of their migratory habits and value as human
     food, carry isotopes off the Savannah River plant site. (5) Initiate food
     chain studies involving the dominant and problem species.  (6) Develop a
     model of the swamp forest to serve as a guide for the design of future
     studies and the identification of important components of the systems and
     transfer coefficients between these compartments of the model.
772. Saville, T.  1966.  Study of Estuarine Pollution Problems on a Small Un-
        polluted Estuary and a Small Polluted Estuary in Florida.  Univ.
        Florida Engineer Progress 20(8):Bull. Ser. No.  125.  210 pp.

        A three and one-half year study designed to evaluate the ability of  an
     estuary to assimilate waste under natural and controlled conditions.
     (WE)
773. Schlesinger, W.H. 1978.  Community Structure, Dynamics, and Nutrient Cycl-
        ing in the Okefenokee Cypress Swamp Forest.  Ecological Monographs.
        48(4):43-65.
774. Schroeder, G. L.  1975.  Some Effects of Stocking Fish in Wastewater
        Treatment Ponds.  Water Research 9:591-595.

        Oxidation ponds or sewage lagoons often present characteristics  of
     natural water bodies in an extreme state of ecological imbalance.   Excess
     nutrients of the decaying waste give rise to plankton blooms which  subse-
     quently die.  In such conditions dissolved oxygen concentrations  (DO)  in
     the water are often low and the pH depressed.  The addition of  fish to
     ponds which twice a week received, per ha, wastes with a 5 day  BOD  of  up
     to 800 kg (20 C) and 5600 kg solids, reduced plankton and benthic popu-
     lations, increased the average DO, and raised the pH.  All of these

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     changes improve the effectiveness of a waste treatment pond for reducing
     BOD and removing nutrients from the water.  Bacteria concentrations were
     as much as 15 times lower in treament ponds stocked with fish as compared
     with unstocked ponds.  Lower bacteria concentrations however reduced the
     rate of loss of COO in the solids of the waste.  (AA)
775. Schrouder, J. D.  1974.  Forage Production in Municipal Wastewater Treat-
        ment Lagoons.  Michigan DNR, Fisheries Div., Grand Rapids.
776. Schulze, K.L.  1966.  Biological Recovery of Wastewater.  J. Water Pollut.
        Control Fed.  38:1914.
777. Schurr, K., and J. M. Golonbeck.  1974.  Calculated Yield of Sewage
        Lagoon Biomass.  A Plan for Production, and Some Problems Inherent in
        Using Biomass or Lagoon Water for Production of Food and Fiber, pp.
        102-110.  In Wastewater Use in the Production of Food and Fiber.  U.
        S. Environmental Protection Agency.  Ada, OK.
778. Schwartz, H. G. , Jr., and B. S. Shin.  1980.  Combined Aquaculture Sys-
        tems for Municipal Wastewater Treatment - an Engineering Assessment,
        pp. 81-104.  In S. C. Reed and R. K. Bastian (eds.), Aquaculture
        Systems for Wastewater Treatment: An Engineering Assessment.  EPA
        430/9-80-007.  U.S. Environmental Protection Agency, Office of Water
        Program Operations.  Washington, DC.

        The purpose of this paper is to assess the current status of combined
     aguaculture systems developed for municipal wastewater treatment and
     determine if these concepts are ready for routine use and, if not, what
     must be done to make them a reality. A combined aquaculture system, or
     polyculture system, is defined for this paper as one in which major
     wastewater treatment work is carried out by several different levels of
     aquatic organisms.  It includes wastewater treatment ponds with a com-
     bination of components such as mechanical elements, aquatic plants,
     invertebrates, and fish.
779. Schwegler, B.R., Jr.  1977.  Effects of Secondary Effluent on the Plant
        and Soil Components of a Northern Wetland Ecosystem, pp. 312.  In;
        R.H. Kadlec, D.L. Tilton, and J.A. Kadlec (eds.). Feasibility of Util-
        ization of Wetland Ecosystems for Nutrient Removal from Secondary Mun-
        icipal Wastewater Treatment Plant Effluent, Semi-Annual Report No. 5.
        Univ. of Michigan, Ann Arbor.

        Continuous flow bioassay chambers were used to determine the effects of
     sewage effluent on the growth rates and nutrient concentrations of fila-
     mentous algae in a northern Michigan wetland.  Bioassay growth rates were

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     2656 mg dry wt. m~2 day ~1 at the site of effluent addition and 85 mg
     dry wt. nT2 day~^ at the control site.  Phosphorus concentrations were
     significantly higher in algae grown at the pipeline site (0.47%) than in
     algae grown at the control site (0.12%).  These differences in growth
     rates and phosphorus concentrations represent 0.96 g m~2 P in the algae
     at the effluent addition site and 0.006 g m~2 P in the algae in the con-
     trol site.  Calculations for examining uptake rates are presented and dis-
     cussed.  Suspended sediments limited the photic zone to the upper 3-5 cm
     of the water column.  (AA)
780. Schwegler, B. R., Jr.  1978.  Effects of Sewage Effluent on Algal
        Dynamics of a Northern Michigan Wetland.  Master's Thesis.  Univ. of
        Michigan, Ann Arbor.
781. Schwegler, B. R., and C. J. Richardson.  1977.  Effects of Nutrient
        Additions on Algae in a Central Michigan Wetland.  Bulletin of the
        Ecological Society of America.  Houghton Lake, MI.
782. Sea Pines Public Service District.  1981.  Wastewater Management Study:
        Effluent Reuse and Disposal.  Volume 1 Technical Report.  Hilton Head
        Island, SC.

        Among other methods of effluent disposal, an experimental wetlands
     application system will be constructed to evaluate the potential for this
     method of effluent disposal.  A 4' by 10' unlined ditch will be excavated
     across the wetland.  Effluent will be discharged to the ditch via small
     diffuse pipes at 100' spacing.
783. Searsbrook, E., and D. W. Davis.  1971.  Effect of Sewage Effluent on
        Growth of Five Vascular Aquatic Species.  Hyacinth Control J. 9:26.
784. Seidel, K.  1966.  Purification of Water by Higher Plants.  Natur-
        wissen-Schaften (German) 12:289-297.
785. Seidel, K.  1971a. Wirkung Hoherer Pflanzen Auf Pathogene Keime  in
        Gewassern.  Naturwissenschaften 58(4):150-151.
786. Seidel, K.   1971b. Macrophytes as Functional Elements  in  the Environment
        of Man.   Hydrobiologia  12:121-130.

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787. Seidel, K.  1976.  Macrophytes and Water Purification, pp. 109-121.  In
        J. Tourbier and R. W. Pierson, Jr. (eds.), Biological Control of Water
        Pollution.  Univ. of Pennsylvania Press, Philadelphia.
788. Seidel, K., and R. Kickuth.  1967.  Biological Treatment of Phenol-con-
        taining Wastewater with Bulrush (Scirpus lacustris L.).  Wassersirt-
        schaft-Wassertechnik.  17(6):209-210.
789. Sell, M.G.  1977.  Modeling the Response of Mangrove Ecosystems to Herbi-
        cide Spraying, Hurricane, Nutrient Enrichment, and Economic Development
        Ph.D. Dissertation Dept. of Env. Engr. Sci.  University of Florida,
        Gainesville.  389 pp.
790. Semkin, R. G., A. W. McLarty, and D. Craig.  1976.  A Water Quality Study
        of Cootes Paradise.  Water Resources Assessment, Technical Support
        Section, Ministry of the Environment, West Central Region.  Toronto,
        Ontario, Canada.

        Studies were performed on a deep-water, channelized wetland in
     Ontario, Canada, that received a wastewater discharge.  Nitrogen removal
     was good.  Heavy metals were present in sediment samples collected down-
     stream from the discharge.  (EP)
791. Shannon, E. E. et al.  1976.  Polychlorinated Biphenyls (PCB's) in
        Municipal Wastewaters.  Research Rept. No. 49, Ministry of the Envir-
        onment, Ontario, Canada.
792. Shedden, R.D.  1974.  The Significance of Biological Denitrification of
        Nitrate Polluted Ground Waters in the Anaerobic Sediments of Wetlands.
        Ms.  Thesis.  Tufts Univ., Newton, Mass.
793. Sheffield, C.W.  1967.  Water Hyacinth for Nutrient Removal.  Hyacinth
        Control J.  6:27-30.
794. Shelef, G.  1978. The Combination of Algal and Anaerobic Waste Treatment
        in a Bioregenerative Farm System. United Nations Univ. Conference on
        "The State of the Art of Bioconversion or Organic Residues for Rural
        Communities", Guatemala City, Guatemala, 13-15 Nov. 1978.

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 795. Shelef, G. et al.  1977.  Economic Aspects of Microalgae Production  on
         Sewage.  GSF Workshop on Microalgae for Food and Feed - A Status  Analy-
         sis, Munchen, Oct. 17-18, 1977.


 796. Shelef, G. et al.  1980.  Algal Mass Production as an Integrated Part of
         Wastewater Treatment and Reclamation.  In; Proc. Int. Symp. Prod, and
         Use Algal Biom.,  Shelef and Boeder (eds.), Elsevier, Amsterdam.


 797. Shelef, G., R. Moraine, and E. Sandbank.  1977.  Physico-chemical Treat-
         ment of Pond Effluents for Unrestricted Agricultural Reuse with
         Recovery of Algae as Protein Source: Pilot and Field Scale Exper-
         iments.  Technion-Israel Inst. of Tech., Haifa.  Sherman Environmental
         Engineering Research Center.  17 pp.

         Alum flocculation coupled with flotation was found to upgrade stabil-
      ization pond effluents to quality permitting reuse for unrestricted
      irrigation.  Removal of phosphate by the alum permits seasonal impound-
      ment of the clarified effluent with minimal eutrophication.  The by-
      product algal sludge can serve as a high quality protein source  for
      poultry and fish, replacing over a quarter of the soymeal in broiler
      ratios as well as over three-quarters of the fishmeal in carp rations.
      The combined values  of waste disposal, renovated water, and feed protein
      make the system highly economical.  (NT)


1091. Shiaris, M.P. In press.


 798. Shih,  S.F.  1981. Upland Marsh for Water Quality Control. Journal of the
         Environmental Engineering Division, Proceedings of the ASCE,  107  (EE4):
         861-864.

         Although many studies have investigated the use of vascular plants to
      improve the water quality of fresh water marshes,  previous researchers
      have not emphasized  the application of models to determine plant nutrient
      uptake in marshes.  This study used time series analysis to analyze plant
      nutrient uptake, used the F-test as the statistical criterion to determine
      the possibility of any component existing in series,  and demonstrated the
      application of time  series analysis to interpolate missing data.  Since
      plant  nutrient uptake is a function of time,  time series analysis can be
      used to observe the  trends,  cyclical movement, and residual pattern  of a
      water  quality system in marsh areas.  Total phosphate data taken at 4 hour
      intervals over a 5 day period in September was used to demonstrate the
      application of time  series analysis.  The Fourier coefficients, amplitude,
      phase  angle,  and percent of  variance were determined for each harmonic at
      three  sampling sites.   The Fourier analysis showed that three harmonics
      explained over 83 percent of the variance.  The amplitudes were much
      smaller in the marsh than in the slough,  confirming the reduction of total
      phosphate after the  water flowed through the marsh zone.  Interpolation of
      missing data  based on time series function resulted in somewhat higher
      values than estimates based  on conventional linear function.  The F-test
      for the cyclic function component of the marsh site was significant at the
      0.01  level, demonstrating the existence of the cyclic component in the
      marsh  zone system.

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799. Shih, S. F., A. C. Federico, J. F. Milleson, and M. Rose.  1979.
        Sampling Programs for Evaluating Upland Marsh to Improve Water
        Quality.  Transactions of the American Society of Agricultural
        Engineers 22(4):828-833.
800. Shindala, A.  1972.  Nitrogen and Phosphorus Removal from Waste Waters.
        Part 1.  Water & Sewage Works Journ. Jun:66-71.
801. Shore, F., W. Van Zandt, and W. Smith.  1977.  Water Hyacinths for
        Removal of Toxaphene from Water.  J. Miss. Acad. Sci., 22(supp.):
        17-22.
802. Siccama, T. G., and E. Porter.  1972.  Lead in a Connecticut Salt Marsh.
        Bio Science 22(4):

        The author reports that a salt marsh removes and stores lead from
     inflowing waters washed from urban pavements.
803. Sickels, F. A., R. L. Simpson, and D. F. Whigham.  1977.  Decomposition
        of Vascular Plants in a Delaware River, Freshwater Tidal Marsh exposed
        to Sewage Spray Irrigation.  Bulletin of the New Jersey Academy of
        Science 22(2):41-42.
804. Silvo, O. E. J.  1972.  Some Experiments on Purification of Wastewaters
        from Slaughterhouses with Sphagnum Peat, pp. 311-318.  In Fourth Int.
        Peat Congr. Proc., Ontaniemi, Finland.
805. Simmers, J. W., B. L. Folsom, Jr., C. R. Lee, and D. J. Bates.  1981.
        Field Survey of Heavy Metal Uptake by Naturally Occurring Saltwater
        and Freshwater Marsh Plants.  U.S. Army Engineer Waterways Experiment
        Station. Environmental Lab Vicksburg, MS.  161 pp.

        A natural marsh heavy metal survey was conducted to establish a base-
     line for comparison to plant concentration and uptake data from con-
     taminated and perturbated sediments.  Extensive field sampling of natural
     marshes was conducted along the east and gulf coasts of the United
     States, and along the shores of the Great Lakes.  Spartina alterniflora.
     was collected from the saltwater marshes and Cyperus species were col-
     lected from the freshwater marsh areas.

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        Results indicated that, in the saltwater marsh, Mn and Zn concen-
     trations were slightly lower than those previously determined in green-
     house and disposal site studies with contaminated sediments, while the
     concentrations of the remainder of heavy metals were similar.  Calcula-
     tion of total uptake values indicated that only Cd may be of concern.  Cd
     levels in Cyperus species in naturally occurring marshes were similar to
     those of a greenhouse flooded (reduced) environment.  Fe and Mn were
     generally present in lower concentration in the natural marsh than in the
     greenhouse plants grown on contaminated sediment, while the remainder of
     the heavy metals examined were present in higher concentrations.
        This investigation forms the basis for the conclusion that marsh
     plants grown on contaminated dredged material in a flooded environment do
     not bioconcentrate excessive or even significantly higher levels of toxic
     metals than those same plant species in naturally occurring marshes.
     (AA)
806. Simpson, H. J.  1979.  Dredge Spoils and Sewage Sludge in the Trace Metal
        Budget of Estuarine and Coastal Waters.  National Technical Informa-
        tion Service Report PB-295-711.  224 pp.

        Human-induced increases in heavy metal and PCB pollution in the Hudson
     River estuary were monitored.  Anthropogenic increases of 3-6 times
     natural levels were detected for Zn, Cu, and Pb.  Measurements indicate
     that the predominant source of organic pollution in the estuary  is
     sewage.  A new technique developed to trace the distribution of  cellulose
     in coastal sediments is described.  Management alternatives that could
     minimize adverse impacts of sewage disposal on the estuary are con-
     sidered.  (EL)
807. Simpson, R. L.  T979.  The Effects of Sewage Spray Irrigation in  a
        Freshwater Tidal Wetland.  II.  Decomposition Studies.  Presented  at
        the Annual Meeting of the American Institute of Biological Sciences,
        held 12-17 August 1979, at Stillwater, OK.
808. Simpson, R. L., and D. F. Whigham.  1978.  The Impact of Treated Sewage
        on Water Quality of a Delaware River Freshwater Tidal Wetland.   Paper
        presented at Annual Meeting American Society of Limnology  and Ocean-
        ography.
809. Simpson, R.L. and R.E. Good. Fluxes of Heavy Metals  in Freshwater Wetlands
        Rutgers Univ., NJ  Manuscript.

        The project objectives are to identify  specific compartments  of heavy
     metal (especially Pb, Cd, Zn) accumulation in freshwater  tidal wetlands,
     monitor fluxes among these compartments, and determine the  upper thresh-
     olds of these compartments for heavy metal content.  Phase  one of the
     study is to identify sites of heavy metal  accumulation in the  study area,

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     determine seasonal changes in heavy metal concentrations  in  the plants,
     sediments, and detritus, and the role of water in moving  heavy metals  to
     and from the sediments.  Phase two of the study will determine the  fate  of
     heavy metals added to the wetland experimentally.
        The results of this study should identify the way in which freshwater
     tidal wetlands act as "sinks" for heavy metals and the role  these wetlands
     play in immobilizing these pollutants.
810. Simpson, R. L., D. F. Whigham, and R. Walker.   1978.  Seasonal Patterns
        of Nutrient Movement in a Freshwater Tidal Marsh, pp. 243-258.   In R.
        E. Good, D. F. Whigham, and R. L. Simpson  (eds.), Freshwater Wetlands:
        Ecological Processes and Management Potential.  Academic Press,  New
        York.

        The distribution and movement of dissolved 02, CO2, NO3~N, NK^-N and
     PO4~P in the surface waters of a freshwater tidal marsh were studied.
     Tidal action, particularly periodic inundation  and flushing, resulted in
     distinctly different patterns of nutrient distribution in the major wet-
     land habitats.  Inorganic N and PO4~P were accumulated in the marsh dur-
     ing summer with emergent vegetation appearing to play an important  role in
     the uptake and retention of nutrients.  Although evidence is accumulating
     that some N and P may be translocated into belowground parts by several
     perennial macrophytes, most is rapidly leached  after death of vascular
     plants with up to 80% of the total N and even more of the P lost within 1
     month.  In pond-like areas where filamentous algal blooms develop follow-
     ing the fall dieback of vascular plants, inorganic N and PO4~P levels
     remain depressed through the winter and spring. On the basis of presently
     available evidence, it appears almost all habitats of freshwater tidal
     marshes may be sinks for inorganic N and PO4~P  during the vascular  plant
     growing season and that certain habitats may continually function as
     sinks.  (AA)
811. Simpson, R.L., D.F. Whigham, and F. Sickels.   1979.  The Effect of  Sewage
        Spray Irrigation in a Freshwater Tidal Wetland II.  Decomposition
        Studies.  Paper Presented at Annual Meeting Ecological Society of
        America.
812. Sinclair, L.R. and R.B. Forbes, 1980.  Nutrient Removal from Drainage
        Waters with Systems Containing Aquatic Macrophytes.  Transactions of
        the ASAE.  23(5):1189-1194.

        Water quality was measured at the inflow and outflow of three treat-
     ment systems to determine their effectiveness in removing nutrients from
     agricultural drainage water.  The study was adjacent to Lake Apopka, Flor-
     ida, which has undergone eutrophication during the past 50 years from
     nutrients added by drainage water from muck farming.  A natural swamp was
     not very effective in improving water quality.  Turbidity, sulfate, pH,
     and nitrates were slightly reduced, and alkalinity, ammonia, phosphate,
     tannin-lignin, carbon dioxide, and temperature increased slightly. Oxygen

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     concentration decreased significantly.  An anaerobic system, floating
     water hyacinths planted in a 16.2 hectare reservoir, improved water qual-
     ity slightly through the growing season.  Nitrates, oxygen, phosphates,
     and sulfate decreased; other changes were not statistically significant.
     This method was most effective of the three in nitrate removal.  After the
     water hyacinths started to decay, water quality degenerated.  A 0.4 hec-
     tare recycle reservoir containing Na j us, submerged plants, removed nutri-
     ents very effectively.  In this aerobic process, alkalinity, ammonia,
     carbon dioxide, phosphate, tannin-lignin, turbidity, and sulfate decreased
     significantly, and oxygen and pH increased significantly.  This system was
     effective in winter.  The author speculated that a combination of naiad
     and hyacinth systems might produce improved results.  The possibility of
     using emergent plants such as cattails was also mentioned.
813. Sinha, S.N., and L.P. Sinha.  1969.  Studies on Use of Water Hyacinth Cul-
        ture in Oxidation Ponds Treating Digested Sugar Wastes and Effluent  of
        Septic Tank.  Environmental Health 11:197-207.
814. Slaughter, R., and I. Wile.  1979.  Natural and Artificial Marshes  for
        Sewage Treatment in Ontario.  In; J.C. Sutherland, and R.H. Kadlec
        (eds.), Wetlands Utilization for Management of Community Wastewater.
        Abstracts of a Conference held 10-12 July  1979 at Higgins Lake,  MI.

        To determine potential of wetlands for year-round sewage treatment in
     Ontario, an experimental study using both a natural and artificial  wetland
     will commence in 1979.  The study will attempt to define the degree of
     pretreatment of the waste required prior to discharge to the wetlands,
     maximum loading rates and required retention  periods to establish optimal
     operational limits for the systems.  Also operational costs and  land
     requirements for wetland treatment systems will be assessed.
        The artificial marsh system will consist of four separate 980 m2 cells,
     two of which will be channelled to permit harvesting of the cattails,
     Typha spp.  Two of the cells will receive raw aerated sewage and the
     remaining two will be treated with sewage lagoon effluent.  The  operating
     range will provide for retention periods between 2 and 10 days and  areal
     loading rates between 1 and 10 cm/day. An additional marsh/pond/channelled
     marsh system will be operated in series and treated with lagoon  effluent.
     The marsh cell of this system will operate at depths of 5 to 30  cm  and  1
     to 4 day retention periods.  The pond will be maintained at a 90 cm depth
     with a retention time of 2 to 9 days and the  channelled marsh at 5  to 30
     cm depths with 0.5 to 2 day retention periods.  A small 30 m2 cell  with
     an impermeable lining will be used for evapotranspiration studies.   Con-
     struction of the facility is scheduled to commence in July 1979, adjacent
     to the town of Listowel sewage treatment lagoons.
        In addition, a 1400 m2 plot, adjacent to the town of Bradford sewage
     treatment plant has been fully instrumented to permit detailed measure-
     ments of its water balance.  The site is densely populated by both  Typha
     latifolia L. and Typha ancpastifolia L.  Background nutrient budgets for
     the plot will be monitored for two years prior to the addition of
     partially treated wastes from the sewage treatment plant.  (AA)

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815. Sloan, L.  1971.  Sewage Oyster-Farm.  Technology Review 73(6):72.
816. Sloey, W. E., F. L. Spangler, C. W. Fetter,Jr. 1976. Productivity and
        Phosphorus Distribution in Natural and Artificial Marshes in Wis-
        consin.  Aquatic Ecology Newsletter 9:20-21.
817. Sloey, W. E., F. L. Spangler, and C. W. Fetter, Jr.  1978.  Management of
        Freshwater Wetlands for Nutrient Assimilation, pp. 321-340.  In R. E.
        Good, D. F. Whigham, and R. L. Simpson  (eds.), Freshwater Wetlands:
        Ecological Processes and Management Potential.  Academic Press, New
        York.

        Nutrient transformation processes such  as sorption, coprecipitation,
     active uptake, nitrification and denitrification remove P and N from the
     free-flowing water of a wetland and transfer them to the substrate and
     biota for storage.  Advantage is being taken of these processes by using
     wetlands to treat sewage in Germany, Holland, Finland, and other European
     countries.  In the U.S.A., experimental application of cultural water
     (wastewater) to peatlands in Michigan, tidal marshes in Louisiana and New
     Jersey, cattail marshes in Wisconsin, cypress domes and sawgrass in
     Florida and many more have shown promising results.  Denitrification may
     remove up to 3.5 kg N/ha/day and as much as 20 g P/m~ may be detained
     a growing season.  Natural release of nutrients between growing seasons,
     however, either restricts application periods, or demands management of
     the wetland systems to regulate such releases.  The most obvious manage-
     ment tool, plant harvesting, removes only  a few grams of P per square
     metre per year which is usually  20% of that detained.  Most of the
     remainder is in the substrate-microbial compartment and is subject to
     between-season washout.
        Other management techniques used and proposed, such as dikes, drains,
     and intermittent application, relate to manipulating the hydraulics of
     the system to optimize conditions for assimilative biogeochemical pro-
     cesses and to prevent washout from reaching surface waters.  Chemical
     treatment to reduce the surplus of P has also been considered.  Manage-
     ment options for a specific wetland will depend to a large extent upon
     that particular hydrologic regime.  Riverine systems have different
     hydraulic patterns than lacustrine or palustrine systems.  Flow-through
     systems cannot be managed like influent or seepage systems.   Already,
     changes in the biota of some experimentally treated wetlands indicate
     undesirable disturbances of these valuable natural resources.  Caution in
     widespread use of natural wetlands to treat waste at this time is advised
     and careful monitoring of all biotic communities in experimental programs
     is essential.  Artificial marshes and peat filters offer feasible alter-
     natives to other treatment methods for small systems and do not endanger
     natural wetlands.  (AA)
818.  Small, E. and J.D. Gaynor.  1975.  Comparative Concentrations of Twelve
        Elements in Substrates and Leaves of Scirpus yalidus and Other Aquatic
        Plant Species in a Sewage Lagoon in Unpolluted Habitats.  The Canadian
        Field Naturalist, 88, 1975, pp. 44-45.

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        Concentrations of 12 elements were examined in vegetation and associ-
     ated soils and water of 12 rooted aquatic plant species growing in the
     lagoon of a sewage treatment plant, and elsewhere in unpolluted habitats.
     Near the point of discharge of effluent into the lagoon, the substrate
     accumulated sludge, with very high concentrations of Cl, Zn, and Cu, and
     also notably high concentrations of P, Fe, and Ca.  The only rooted aquat-
     ic plant species successfully colonizing this area was Scirpus valjldus
     which accumulated significantly higher concentrations in the leaves of P,
     Fe, Cl, N, Na, and Mg compared with the same species in nonsludge areas of
     the lagoon, and in unpolluted habitats.  Eleven additional aquatic plant
     species were found in the lagoon, rooted in sandy sediment adjacent to the
     sludge area.  These species appeared excluded from the sludge area.  Con-
     centrations of the 12 elements examined in substrates and leaves of these
     species growing just outside the sludge area did not differ notably in
     comparison with concentrations in unpolluted habitats.  (AA)
819. Small, M.M. 1967.  Fresh Water from Sewage in Long Island.  Brookhaven
        National Lab, Upton, NY.  NTIS No. BNL-21371.  April, 1967.  16 p.

        Sewage and cesspool contents were used to recharge groundwater in
     studies at the  Brookhaven National Laboratory in Upton, New York.  The
     wastewater purification system was a sloping grass meadow draining  into  a
     planted marsh and eventually into a fish-stocked pond.  The sewage  was
     applied to the meadow where it permeated the soil and plant roots.  The
     wastewater passed through the marsh soils, plant stalks, and root before
     flowing into the pond.  Overflow from the pond was usable as potable
     water.  The same degree of purification could be attained by a  marsh/pond
     system where meadowland is not available and there is a high population
     density. Both systems were completely lined so the wastewater could not
     percolate into the groundwater before purification.  The system produced
     no sludge or odor. The marsh/pond sewage treatment system costs an  esti-
     mated $1,440,000 compared to $640,000,000 for a municipal sewage treatment
     plant.
820. Small, M.M.  1975a.  Brookhaven1s Two Sewage Treatment Systems.   Compost
        Science 16(5):7-10.

        Now operating at Brookhaven National Lab on Long Island, NY,  are  two
     novel sewage treatment systems, each of which processes  10,000 gpd domes-
     tic sewage and returns drinkable water to the groxindwater  supply.  Neither
     system produces any sludge.  The meadow-marsh-pond system  and the  marsh-
     pond system, which are in competition to determine the least expensive to
     build and operate, are described.   (EL)
821. Small, M. M.  1975b. Meadow/Marsh Systems  as  Sewage Treatment  Plants.
        Brookhaven National Laboratory, Upton,  NY.  39 pp.   (NTIS  Kept.
        BNL-20757).

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        All parameters of the marsh/pond and the meadow/marsh/pond  systems
     that have been measured to date indicate that both systems produce water
     suitable for groundwater recharge or other reuse without  a public health
     hazard.  This discharged water can be made to satisfy both effuent and
     drinking water standards.  Both systems can be recommended for use as
     sewage treatment plants, water producers, and farms for sewage loads from
     10,000-1,000,000 gpd.  Land availability and its terrain  will  be prin-
     cipal determinants in choosing between the systems for a  particular
     application.  (EL)
822. Small, M. M.  1976a. Marsh/Pond System.  Brookhaven National Laboratory,
        Upton, NY.  28 pp.

        Data from the first year of operation of the Marsh/Pond  semi-works  at
     Brookhaven National Laboratory are reported.  Sewage is first aerated,
     then passed through a marsh, then through a pond, and finally,  is  spread
     on a forest floor to recharge groundwater supplies.  Since  the  system  is
     still under study, detailed conclusions would be premature; however, it
     was observed that a Marsh/Pond system will convert weak sewage  to  water
     suitable for recharge through a vegetated sandy loam.
823. Small, M. M.  1976b. Marsh/pond Sewage Treatment Plants, pp.  197-214.   In
        D. L. Tilton, R. H. Kadlec, and C. J. Richardson  (eds.), Proceedings
        of a National Symposium on Freshwater Wetlands and Sewage  Effluent
        Disposal.  Univ. of Michigan, Ann Arbor.

        At Brookhaven National Laboratory today there is  operating a  10,000
     gallon per day sewage treatment system that consists only  of  a marsh and
     a pond.  The influent to this system is raw sewage blended with  septage
     and sludge.  The effluent is potable water.  The system is odor,  rodent,
     fly, and mosquito free.  It produces no sludge nor airblown particles.
     It is a pleasant site to have next door, complete with fish,  birds, and
     wildlife.  It produces a useful annual crop which is salable.  This
     project is the prototype of similar systems that are proposed for com-
     munities of up to 10,000 people.  They can be built  at a cost of  about
     60t/gal day and operated at less than one man year expense.   (AA)
824. Small, M. M.  1976c. Data Report, Marsh/Pond System.  Preliminary Rept.
        No. 50600 to the U.S. Energy Research and Development Administration.
        Brookhaven National Laboratory, Upton, NY. 28 pp.

        This report summarized data compiled after one year's operation of  an
     experimental marsh/pond system constructed at Brookhaven National Labor-
     atory, Upton, New York, in 1973.  This facility was one of a  number of
     facilities used as prototypes to develop natural systems for  the reno-
     vation of wastewater.  Because the system was under study at  the time
     this report was written, no detailed conclusions were drawn except the
     observation that a marsh/pond system will convert weak sewage into water
     that is suitable for recharge through a vegetated sandy loam.   (EP)

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825. Small, M. M.  1977.  Natural Sewage Recycling Systems.  Rept.  to  the U.S.
        Energy Research and Development Administration.  Brookhaven National
        Laboratory, Upton, NY. 37 pp.

        This paper reviews the work done at Brookhaven National Lab, in  the
     development of natural systems which produce potable water from sewage.
     Experiments with two prototype systems, a marsh/pond and meadow/marsh/
     pond system, are described, and performance data are presented in detail
     for the marsh/pond system.  Empirical interpretation of results achieved
     to date are suggested for the use and design of marsh/ponds  as natural
     sewage recycling systems.  (WE)
826. Small, M.M., 1978a. Artificial Wetlands as Non-point  Source Wastewater
        Treatment Systems.  In; Drew, M.A.  (ed.), Environmental Quality  Through
        Wetlands Utilization.  Proceedings  of  a Symposium  held 28 February - 2
        March 1978 at Tallahassee, FL, p. 171-181.

        A comparison of nutrient data from  the Taylor Creek,  Florida farm
     wastewater drains with characteristics of effluent  from  the Brookhaven,
     New York lowland system models shows about the  same concentration on NC>3
     -N and and PC^-P in the discharges  from both systems.  An analysis  of
     each system indicates that both operate at a higher input rate  of phos-
     phates than the natural biological  communities  are  removing.   In the case
     of the Brookhaven models, the 2 ppm of PC^-P in the effluent  is insig-
     nificant; in the case of drains leaching  to Lake Okeechobee,  the 2  ppm is
     excessive.
        Over four years experimentation  with the meadow/marsh/pond model indi-
     cates that modifications of this design,  with care  in operation, will
     remove both inorganic nitrogen and  phosphorus to levels  which no longer
     constitute a threat to the Lake.  If it is true that  the annual dump of
     PC>4 into the Lake is contributed principally by runoff from  the farms,
     that dump can be eliminated by detaining  the runoff at each  farm by a
     marsh/pond.
        For low-cost, low-energy detention  systems for this purpose, it  is
     suggested that the Demonstration Project  consider,  at each farm, a  marsh/
     pond combination which is designed  to  remove some water  by evaporation, a
     lot of nitrogen by denitrification, and the major portion of  available
     phosphate by the harvest and removal from the basin of indigenous aquatic
     weeds.
        This paper develops a module  for such  a  lowland  nutrient  removal
     system.  Construction costs depend  on  size but  will be below $2800  per
     acre of farm.  Operating costs are  under  18^/1000 gallons.   (AA)
827. Small, M. M.   1978b. Wetlands  Wastewater Treatment Systems,  pp. 141-149.
        In H. L. McKim  (ed.),  State of  Knowledge in Land Treatment of
        Wastewater.  Volume 2.   Proceedings of an International Symposium
        Sponsored  by the U.S.  Army  Corps of Engineers,  held 20-25 August, 1978.

         Two artificial wetlands sewage reclamation systems are examined from
     an engineering viewpoint.   The systems appear to be technically superior
     to conventional secondary treatment plants and the efficiency of such

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     systems can be compared to that of advanced wastewater treatment plants.
     Estimated construction/ operation, and maintenance costs of  artificial
     wetlands are far less than equivalent costs are  for either conventional or
     advanced treatment plants in the 38-3800 m-^/day  flow  range.  Energy
     consumed in artificial wetlands construction is  estimated to be a  small
     fraction of the total energy required to build a conventional  or advanced
     treatment plant.  Design constraints that are necessary to gain the  sewage
     reclamation are presented.  (EL)
828. Small, M.M.  1979.  Marsh/pond Sewage Treatment Plants II.   In; J.C.  Suth-
        erland and R.H. Kadlec  (eds.), Wetland Utilization for Management  of
        Community Wastewater.  Abstracts of a Conference held 10-12 July 1979
        at Higgins Lake, MI.

        A general description of artificial wetlands, built and operated at
     Brookhaven National Laboratory for the treatment of sewage,  was presented
     at the Freshwater Wetlands and Sewage Effluent Disposal Conference, May
     10-11, 1976 at the University of Michigan.  In May, 1976 an  artificial
     Marsh/Pond and an artificial Meadow/Marsh/Pond had been operated  as con-
     tinuously on-line treatment systems for about 9 months and some prelimin-
     ary observations were reported - some prescient - others dead wrong.  This
     is an update three years later.
        The net conclusion is that artificial Marsh/Ponds work.   They  make an
     attractive wetlands which can be constructed almost anywhere.  They will
     not dump raw sewage if overloaded, will generate no sludge for separate
     disposal and do not produce objectionable odors, vermin or disease vec-
     tors.  Less than 20 acres of land is sufficient to treat 1 MGD of normal
     domestic sewage in a Marsh/Pond which will cost about $1 million  to build.
     They are an energy bargain for today.  (AA)
829. Small, M., and C. Wurm. 1977.  Meadow/Marsh/Pond System.  Data Report to
        U.S. Energy Research and Development Administration.  Brookhaven
        National Laboratory, Upton, NY.  33 pp.

        This report summarizes 13 months' operating data, August  1975-August
     1976, from the Meadow/Marsh/Pond system, one of two natural  sewage-treat-
     ment systems constructed at Brookhaven National Laboratory.  These sys-
     tems are free of disease vectors, aerosols, and objectionable odors. The
     Meadow/Marsh/Pond system appears superior to the Marsh/Pond  system for
     renovating blends of septage and weak sewage to groundwater  recharge qual-
     ity.  The only conclusion offered at the present time is that the system
     will produce potable water from raw sewage following recharge of pond
     effluent through a vegetated sandy soil.
830. Smith, D.W. 1980.  Water Reclamation and Reuse. J. Water Pollut. Control
        Fed. (Literature Review Issue)  52(6):1242-1284.

        A review of recent (1978-1979) literature on water reclamation and use
     highlights the Water Reuse Symposium held March 25-30, 1979, in Washing-

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     ton, D.C., which dealt with water reuse from the research, development,
     and application viewpoints.  In addition to a summary of general papers,
     the article discusses recent developments in water resources planning
     (models, options in wastewater reuse).
831. Smith, G. R.  1978.  Botulism, Waterfowl and Mud.  British Veterinary
        Journal 134(5):407-411.
832. Smith, L. J., and J. E. Huguenin.  1975.  The Economics of Wastewater
        Aquaculture Systems, pp. 285-293.  In I. E. E. E. Conf. Record of
        Engr. in the Ocean Environment - Ocean '75.  San Diego, CA.
833. Smith, R. C.  1975.  Hydrogeology of the Experimental Cypress Swamps.
        Master's Thesis.  Department of Geology, Univ. of Florida, Gaines-
        ville.  87 p.
834. Smith, W. G., and J. W. Day.  1973.  Enrichment of Marsh Habitats with
        Organic Wastes.  Completion Report.  Louisiana Water Resources Re-
        search Institute.  Baton Rouge, LA.  9 pp.

        Municipal and industrial wastewater have created local problems,
     including eutrophication, plant nutrient loss, discharge-induced die-off
     of algal flora, and changes in chemical and biological character.  Among
     methods used to solve these problems is that of overland runoff, and the
     project is an attempt to incorporate this method into an estuarine envir-
     onment.  A site in the subtropical coastal marsh at Dulac, Louisiana,
     home port facility for a menhaden processor, was selected: an artifi-
     cially enclosed freshwater marsh, totaling about two and one-half square
     miles. (NT)
835. Smithsonian Science Information Exchange Inc.  1976.  Utilization and
        Removal of Nutrients from Fresh Water by Aquatic Vascular Plants.
        Research Information Package BA14, Washington, DC.

        A review of ongoing research supported by various government agencies
     concerning aquatic ecosystems and the ability to remove nutrients from
     fresh water.
        Contains details of ongoing research projects (updated at least every
     90 days). Available from SSIE, Room 300, 1730 M Street, N.W., Washington,
     DC.
836. Snider, J. R., and G. W. Wood.  1975.  The Effects of Wastewater Irriga-
        tion on the Activities and Movements of Songbirds, pp. 20-49.  In G.

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        W. Wood, et al. (ed.), Faunal Response to Spray Irrigation of Chlor-
        inated Sewage Effluents.  Publication 87.  Institute for Research on
        Land and Water Resources Research, Pennsylvania State Univ., Phila-
        delphia.
837. Sodd, A. N.  1981.  Municipal Solid Waste Disposal in Estuaries and
        Coastal Marshlands: Project Summary.  U. S. Environmental Protection
        Agency.  Municipal Environmental Research Lab. Cincinnati, OH.

        See MacGregor et al. (1980) for abstract of this project.
838. Sommers, L. E., C. F. Parker, and G. J. Meyer.  1981.  Volatilization,
        Plant Uptake and Mineralization of Nitrogen in Soils Treated With
        Sewage Sludge.  Tech. Kept. No. 133.  Water Resources Research Center,
        Purdue Univ., Lafayette, IN.  (Available as NTIS Rept. PB81-173940).

        Field and laboratory experiments were conducted to quantify the amount
     of ammonia volatilization taking place after application of sewage sludge
     to the soil surface, evaluate the movement of nitrogen and plant uptake
     from soils treated with sewage sludge, and determine the potential N
     mineralization in a wide range of sewage sludges.  Volatilization of
     NH3-N was found to be dependent upon the method of sludge application,
     initial soil moisture content and sludge pH.  Sorghum plants recovered 7
     to 27% of the total sludge N while the recovery of the total N ranged
     from 8 to 33% considering the N in the soil to a depth of 90 cm.  Signi-
     ficant losses of sludge inorganic N occurred within 3 days of applica-
     tion.  The amount of mineralizable N in 24 sewage sludges was found to be
     proportional to the total organic N content.  The results of the study
     demonstrated the need to use a different N mineralization percentage for
     the the various sludge types.  Recognizing the limitation of the study,
     the recommended N mineralization percentage to use in calculating the
     appropriate sludge application rate for agronomic crops is 25% for raw
     and primary sludges/ 40% for waste activated sludges, 15% for anaer-
     obically digested sludges and 8% for composted sludges.  (WR)
839. Sopper, W. E., and L. T. Kardos (eds.).  1973a. Recycling Treated
        Municipal Wastewater and Sludge Through Forest and Cropland.  Penn-
        sylvania State University Press, University Park, PA.
840. Sopper, W. E., and L. T. Kardos.  1973b. Vegetation Responses to Irriga-
        tion with Treated Municipal Wastewater, pp. 271-294.  In Recycling
        Treated Municipal Wastewater and Sludge Through Forest and Cropland.
        University Park, Penn. State Univ. Press.

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841. Sopper, W.E.  1976.  Renovation of Municipal Wastewater for Groundwater
        Recharge by the Living Filter Method, pp. 269- . In; J. Tourbier, and
        R.W. Pierson, Jr. (eds.), Biological Control of Water Pollution. Univ.
        of Pennsylvania Press, Philadelphia.

        The idea embodied in this concept is to apply wastewater for  direct re-
     charge to the groundwater reservoir. This can be accomplished by control-
     ling application rates and by maintaining normal aerobic conditions within
     the soil.  Under these conditions organic and inorganic constituents in
     the wastewater are removed and degraded by microorganisms, chemical
     precipitation, ion exchange, biological transformation, and biological
     absorption through the root systems of the vegetative cover.  The  utiliza-
     tion of the vegetative cover as an integral part of the system to  comple-
     ment the microbiological and physiochemical systems in the soil  is an
     essential component of the living filter concept and provides for  maximum
     renovation capacity and durability of the system.
842. Sorber, C.A. and K.J. Guter.  1975.  Health and Hygiene Aspects of Spray
        Irrigation.  Amer. J. Publ. Hlth.  65(1):47-51, 1975.

        The following conclusions relative to the health and hygiene effects of
     spray irrigation land disposal of wastewater can be drawn.   1) Many  of the
     detrimental health and hygiene aspects of land disposal should be signifi-
     cantly reduced by proper wastewater pretreatment including  secondary
     treatment, filtration, and complete disinfection. 2) By choosing a land
     disposal site that has from 5-10 feet of continuous fine soil, biological
     contamination of groundwater can be avoided. 3) The probability of inhal-
     ing pathogenic aerosols near a spray irrigation site may be  significant.
     4) If ponding results in spray irrigation areas mosquito breeding is en-
     hanced. 5) In areas where land disposal is the first step in a water
     recycle program, total dissolved solids, sodium, and nitrate buildup in
     the ground water supply can be a problem.
843. Spangler, F.L., C.W. Fetter, Jr., and W.E. Sloey.   1977.  Phosphorus
        Accumulation-Discharge Cycles in Marshes.  Water Resources Bulletin
        Artificial and natural marshes were studied to determine  changes  in
     quality of polluted water passing through them.  Phosphorus  removal
     ranged from zero to 64%.  Removals in the 35% range were common,  however.
     Much of the phosphorus went into sediments and unharvestable plant parts.
     Harvesting vegetation removed 6% of the phosphorus put into  the system.
     Accumulation of phosphorus during the growing season was about 10 g  nr ,
     much of which was flushed out after the onset of freezing weather.   The
     amount of phosphorus flushed out can be prevented from entering a lake or
     stream by handling the marsh discharge in one of three ways: irrigate  on
     land, lagoon to recycle later, treat conventionally if facilities are
     available.  (AA)

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 844.  Spangler,  F. L.,  W.  E.  Sloey,  and C.  W.  Fetter, Jr.   1976a.  Artificial
         and Natural Marshes  as  Wastewater Treatment Systems in Wisconsin, pp.
         215-240.  In D. L. Tilton,  R.  H.  Kadlec,  and C. J.  Richardson (eds.),
         Proceedings of a  National Symposium on Freshwater Wetlands and Sewage
         Effluent Disposal.   Univ. of Michigan, Ann Arbor.

         Artificial marshes were constructed by lining excavations with 20 mil
      PVC, placing gravel  in  them, then planting emergent  vegetation, espe-
      cially softstem bulrush (Scirpus  yalidus).  A control  was used.  Obser-
      vations were made on the effect of the artificial marshes, first on
      secondary, then on primary effluent.   A  study was also done  on water
      quality improvement  by  a polluted natural marsh.
         It was  found that phosphorus removals of  30 - 40% could be expected in
      both natural and  artificial marshes  but  ranged up to 64%. Removal during
      the growing season may  be  negated by  loss during winter and  spring unless
      a management technique  is  developed  and  applied.  Harvesting of plants is
      not a feasible P  removal technique nor does  it influence treatment
      efficiency.  In the  artificial system most of the P  (75%) taken out of
      the wastewater went  into the gravel  and  only about 5%  went into harvest-
      able tissue.  Most of the  purification processes (i.e., BOD  removal and
      nutrient retention)  appear to  be  carried out in the  substrate system
      rather than by the vascular plants.
         Highest BOD removals were 91%  and  77% for secondary and primary
      effluent respectively.   Retention time did not seem  to be critical to BOD
      removal over the  5-hour to 10-day range  for  secondary  effluent.  Efflu-
      ents with  BODs of 5  - 15 mg/1  were produced  by the artificial marshes in
      spite of loss of  up  to  45% of  the water  by evapotranspiration.
         Waste treatment employing emergent vegetation is  probably not as
      energy intensive  as  traditional methods.   Winter application is not
      thought to be possible  in  northern latitudes.   (AA)
845. Spangler, P., W. Sloey, C.W. Fetter.  1976b.  Experimental  Use  of  Emergent
        Vegetation for the Biological Treatment of  Municipal Wastewater in
        Wisconsin, pp. 161-171.  In; J. Tourbier  and R.W. Pierson  (eds.),  Bio-
        logical Control of Water Pollution.

        Native Wisconsin marsh plants were assessed for their ability  to pro-
     vide secondary or tertiary sewage treatment.   A 258-ha natural cattail
     marsh that receives both municipal and industrial waste was investigated;
     a striking water quality improvement  due to  movement through the  marsh  was
     recorded relative to BOD, COD, O-PO4, total-P,  coliform bacteria, and
     turbidity.  The highest concentrations of nutrients were found to occur in
     young plant tissue of emergent species; therefore the effects  of
     harvesting were investigated.  Scirpus acutug  (hardstem bullrush)  and S_.
     yalidus (softstem bullrush) recovered well post harvest while  cattails
     (Typha spp.) did not.  Additionally most P was  associated  with the  root
     rhizomes, and little was harvested in the shoots.  (AA)
846. Spangler, F.L., W.E. Sloey, and C.W. Fetter.  1976c.  Wastewater Treatment
        by Natural and Artificial Marshes.  EPA Technology Series Rpt. No. EPA-
        600/2-76-207.  September.  NTIS No. PB-259 992.  182 p.

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        The uses of artificial and natural marshes as purifiers of municipal
     treatment plant effluent was investigated at various sites in Wisconsin.
     Harvesting plants was not a practical phosphorus removal technique as only
     a small portion of the phosphorus retained by a marsh system is incorpor-
     ated into harvestable plant tissue, and then only in summer.  The level of
     water quality improvement attained suggests that the process may be
     acceptable for polishing effluent from ssptic tanks of single or small
     clusters of buildings.  Marshes remove phosphorus during the growing sea-
     son but release it at other times, thus acting as buffers which may be
     managed to advantage.  The natural marsh was moderately effective in
     removing phosphorus during the growing season but the annual phosphorus
     output was about equal to the input.  Average concentrations of orthophos-
     phate, total phosphorus, conductivity, and total solids were reduced by
     13% or less by passage through some 1900 m of Brillion Marsh.  The phos-
     phorus concentration in water draining from the marsh ranged from a low of
     0.43 mg/1 in September to a high of 11.85 mg/1 in July, and did not follow
     any discernable pattern related either to temperature, rainfall or season.
     Reduction of most parameters did not vary when primary or secondary efflu-
     ent was treated by a marsh.
847. Spehar, R.L. , A.E. Lemke, Q.H. Pickering, T.H. Roush, R.C. Russo,  and J.O.
        Yount.  1981.  Effects of Pollution on Freshwater Fish.  J. Water
        Pollut. Control Fed.  53(6): 1028-1075.
848. Spoon, D.  1976.  Survey, Ecology, and Systematics of the Upper Potomac
        Estuary Biota: Aufwuchs Microfauna, Phase II.  Rept. No. 8.  Washing-
        ton Technical Inst. TVater Resources Research Center, Washington, DC.

        Two model rivers, representing Potomac River sites at Haines Point,
     Blue Plains Sand Piscataway Creek connected to a common reservoir  con-
     taining upriver water, were studied.  Each model consisted of four
     24-gallon aquaria.  In one model at aquarium 2, frozen sewage was  added,
     and in the other, dechlorinated tap water was added.  Four attempts to
     duplicate the May 1974 fishkill, which took place when river to sewage
     flow ratio was 20:1, failed: negative results were obtained with aquaria
     conditions representing river to sewage flow ratios of 20:1, 5:1,  1:1,
     and 4:4.  It was concluded that the fishkill was caused by a toxin in the
     sewage and not a heat shock or the river model closely approximated those
     at Potomac River sites at the same temperature.  Other studies were done
     on a 50 ,000-gallon pilot activated sludge plant before and after hydrogen
     peroxide treatment of a bulking condition.  A new method for counting and
     studying the activated sludge community was devised using thin plastic
     film (Handiwrap) coverslips with plastic slides.  (NT)
849. Stahl, J. B., and D. S. May.  1967.  Microstratification  in Waste-treat-
        ment Ponds.  J. of Water Pollut. Control Fed., 39:72-88.

        A study of stratification of temperature and dissolved oxygen  content
     in a small pond receiving wastes that had  undergone primary treatment

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     showed that sharp stratification  of  these  properties  almost always is
     present.  At a depth of less than two  centimeters,  only  one percent of  the
     surface light remained.  Only  on  heavily overcast,  windy days was  strati-
     fication absent.  On calm, clear  days, temperature  dropped  as much as 20°C
     in 50 cm and oxygen dropped from  300 percent  saturation  at  the surface  to
     3 percent at 5 cm.  These effects were more extreme than those reported
     for other ponds, few of which, however, were  waste  treatment facilities.
     (AA)
850. Stanley Consultants.  1977.  New Orleans-Baton Rouge  Metropolitan Area,
        Louisiana, Water Resources Study.  Wastewater Treatment  by  Marsh
        Application Work Report.  Submitted  to U.S. Corps  of  Engineers, New
        Orleans District.  Atlanta, GA.   147 pp.

          The potential for using study  area marshes for disposal of  secondarily
       treated municipal effluent is discussed and an overview is given of
       methodologies utilized at 18 sites  in the United States.  Brief descrip-
       tions of the dominant vegetation, type of studies being conducted,  and
       results accumulated at each site  are  included.  Three  coastal  marshlands
       in the delta region of the Mississippi River are discussed in  greater
       detail.  Specific characteristics such as climate,  hydrology,  water
       quality, vegetation, sediments, and limitations to  marsh  availability  are
       described for the study sites.  Mechanisms in natural  marshes  and the
       limitations of wastewater application to marshes also  are addressed.
       Based on data gathered in experimental marsh treatment systems, loadings
       and general criteria were established as guidelines to be used in treat-
       ment of marshes.  In cases where  there was a lack of available data,
       criteria were chosen arbitrarily.   (EP)
851. Stanley, N.E.T.L. Thurow, B.F. Russell, and J.F.  Sullivan.   1980.   Geother-
        mal Wetlands: An Annotatd Bibliography of Pertinent Literature.   Idaho
        National Engineering Laboratory.  U.S. Dept. of Energy.  Idaho Falls.

        Literature providing information on natural and artificially created
     wetlands were reviewed.  The objective was to gather pertinent data that
     could be extrapolated to geothermal wetlands.  Applicable studies are pre-
     sented in the form of an annotated bibliography.  Emphasis  was placed on 1)
     the characterization of water quality requirements; 2) tolerance limits  of
     wetland flora and fauna; and 3) the potential effectiveness of aquatic
     vegetation at bioaccumulating solutes inherent in most geothermal waters.
     (AA)
852. Stanlick, H.T.  1976.  Treatment of Secondary Effluent Using  a Peat Bed,
        pp. 257-268.  In; D.L. Tilton, R.H. Kadlec, and C.J. Richardson  (eds.),
        Proceedings of a National Symposium on Freshwater Wetlands and Sewage
        Effluent Disposal. Univ. of Michigan, Ann Arbor.

        The use of a peat bed for systems that operate during the  growing  sea-
     son should give an effluent that will meet most if not all discharge

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     requirements.  If properly designed and installed, the bed should produce
     an effluent with no fecal coliform, and little total coliform, without any
     special disinfection facilities; BOD5 and suspended solids of approximately
     5 mg/1; and nitrogen of less than 10 mg/1.  Design life is expected to be 5
     to 10 years.  With the test results we have, we expect the bed life to be
     close to ten years.  After three years, the treatment efficiency has not
     deteriorated but in fact has increased.
        This type of system can be used to disinfect an effluent and improve the
     quality all year if you do not have stringent requirements on phosphorus
     and nitrogen.  It is ideal for camps and resorts due to the little
     operation and maintenance needed.
853. Stearns, F.  1978.  Management Potential Summary and Recommendations, pp.
        357-363.  In; R.E. Good, D.F. Whigham and R.L. Simpson  (eds.), Fresh-
        water Wetlands: Ecological Processes and Management Potential.  Academic
        Press, New York.

        Wetland management goals fall in three areas: environmental protection,
     recreation and aesthetics, and production of renewable resources.  Most
     wetland management, however, is oriented to several goals  and most wetlands
     serve multiple uses. Several general conclusions concerning wetland manage-
     ment can be made at this time: management decisions should complement nat-
     ural wetland functions whenever possible.  Wastewater should not be applied
     to natural wetlands, other than experimentally, until more is known about
     long-term effects.  The rate of wetland conversion should  be slowed until
     more is known about wetland functions.  Management should  hold to a minimum
     those factors which degrade marsh structure and function.  In creating wet-
     lands, one should remember that plant species differ in their vulnerability
     to stresses.  In modifying wetlands, attempt to avoid disturbances. Natural
     perturbations may occur and management techniques successful at one point
     in the climatic cycle may not be applicable at other times.
854. Stephenson, M., G. Turner, P. Pope, A. Knight, and G. Tchobanoglous.
        1980.  The Use and Potential of Aquatic Species for Wastewater Treat-
        ment.  Appendix A.  The Environmental Requirements of Aquatic Plants.
        Publication No. 65.  California State Water Resources Control Board.
        Sacramento, CA.  380 pp.
855. Steward, K.K.  1970.  Nutrient Removal Capacity of Various Aquatic Plants.
        Hyacinth Control Journal 8:34-35.
856. Steward K.K., and W.H. Ornes.  1973.  Assessing the Capability  of  the Ever-
        glades Marsh Environment for Renovating Wastewater.  Final Rept. Agri-
        cultural Research Service, Fort Lauderdale, FL. 28 pp.

        Everglades vegetation was enriched with simulated effluents  in  order  to
     determine the feasibility of recycling wastewater through  the marshes.

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     Weekly application of nutrients increased assimilation of nutrients by
     the plants but did not increase growth.  This indicated that growth was
     not limited by lack of nutrients.  Only 12% of the applied nutrients were
     assimilated into the vegetation.  Of the amount remaining, 3% was used to
     produce algal blooms, 43% settled to the bottom, 5% remained dissolved in
     the water and 37% was unaccounted for.  The dense algal blooms, which
     were maintained throughout the experiment, were believed to have been
     responsible for the disappearance of several floral components of the
     ecosystem.  Because of the low capacity for nutrient assimilation, and
     because of potential alterations in species composition, it appeared
     unlikely that the marsh system could be used to renovate wastewater.
     (NT)
857. Steward, K. K., and W. H. Ornes.  1975.  Assessing a Marsh Environment
        for Wastewater Renovation.  J. Water Pollut. Control Fed. 47(7):
        1880-1891.

        The authors measured the response of vegetation in the Everglades  to
     enrichment with N and P.  Increases in plant tissue nutrient levels,  but
     no changes in growth, were observed after a single application.  A dense
     algal bloom was produced 4-7 weeks after the start of continuous weekly
     application, which eliminated natural periphyton and Utricularia com-
     munities.  Based on water, sediment, and tissue nutrient levels, the
     researchers concluded that sawgrass had limited ability to remove
     nutrients and that the assimilative capacity of the marsh was exceeded at
     less than the application level of 2.5 kilograms of P per hectare.   (EP)
858. Stewart, E. A., III.  1979.  Utilization of Water Hyacinths for Control
        of Nutrients in Domestic Wastewater - Lakeland, Florida/ pp. 273-293.
        In R. K. Bastian and S. C. Reed  (eds.), Aquaculture Systems for
        Wastewater Treatment: Seminar Proceedings and Engineering Assessment.
        EPA 430/9-80-006.  U. S. Environmental Protection Agency, Office  of
        Water Program Operations, Municipal Construction Division.  Wash-
        ington, DC.

        The use of water hyacinths (Eichhornia crassipes) for removal  of  nutri-
     ents and solids during a demonstration project in Lakeland, Florida  indi-
     cates that removals are as a result of more than hyacinth  uptake.  There-
     fore, if design of a hyacinth system is based upon the uptake and growth
     kinetics of the plants during cool weather periods, it appears that  an
     ample safety margin can be maintained.  The hyacinth growth rates were
     found to be similar to those found in other studies.  A first order  design
     equation based upon the Monod concept using total nitrogen as the limiting
     nutrient is presented. Other aspects of the hyacinth system such  as  har-
     vesting, crop processing, and marketing have been investigated, but  are
     still in need of additional field work before maximum effectiveness  is
     realized.  (AA)
859. Stewart, W. C., and S. A. Serfling.   1979.  The Solar AquaCell System for
        Primary, Secondary, or Advanced Treatment of Wastewaters, pp.  377-412.

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        In R. K. Bastian and S. C. Reed (eds.), Aquaculture Systems for Waste-
        water Treatment: Seminar Proceedings and Engineering Assessment.
        EPA 430/9-80-006.  U. S. Environmental Protection Agency, Office of
        Water Program Operations, Municipal Construction Division.  Wash-
        ington, DC.

        The Solar AquaCell process is an aquaculturally derived wastewater
     treatment system which integrates proven wastewater treatment elements to
     allow greater process control, higher quality effluent, and reduced land
     area as compared to conventional wastewater lagoons.  Three AquaCell pond
     systems, anaerobic, faculative, and aerobic, all containing high surface
     area bio-film devices, can be used in series to achieve primary, second-
     ary, and/or advanced treatment, respectively.  Three years of pilot scale
     demonstrations have shown that conservative design practices can be used
     while also achieving reduced construction, operation and maintenance
     costs, minimal solids handling, low energy demand, and low operator skill
     requirements.  The use of a multiple series of controlled cells allows a
     high degree of design and operational flexibility for meeting various
     effluent quality objectives.  The City of Hercules' Solar AquaCell Treat-
     ment System (California) is also described.  (AA)
860. Stora, G.  1976.  Effects of a Freshwater Effluent on the Development of
        Benthic Communities in a Marine Lagoon.  Bull. Ecol. 7(3) -.275-282. In
        French).

        Since 1966, after installation of the Saint-Chamas hydroelectric plant,
     the Etang de Berre, (France), has received freshwaters of the Durance
     Channel.  This important freshwater inflow representing approximately 4
     times the volume of the lagoon during a year has considerably decreased
     the water salinity.  Studies were made in 1965, 1970 and 1972 to follow
     the evolution of the benthic populations of the Etang de Berre.  Charac-
     terized by typical marine populations in 1964 (bivalves, polychaetes), the
     studies in 1970 show a general degradation of the lagoon populations with-
     out substitution of benthic marine population by a brackish population.
     However, in 1972 the benthic populations were replaced on the littoral
     coast by a characteristic brackish benthic population.
861. Stout, J. P., A. A. de la Cruz, and C. T. Hackney.  1980.  Effects of
        Harvesting in the Annual Net Above-ground Primary Productivity of
        Selected Gulf Coast Marsh Plants, pp. 213-222.  In V. S. Kennedy
        (ed.), Estuarine Perspectives.  Academic Press, Inc., New York.
862. Stowell, R., G. Tchobanoglous, J. Colt, and A. Knight.  1979.  The Use of
        Aquatic Plants and Animals for the Treatment of Wastewater.  Unpub-
        lished Paper.  Dept. Civil Engineering, Univ. of Davis, CA.  24 pp.
863. Stowell, R., R. Ludwig, J. Colt, and G. Tchobanoglous.  1980.  Toward the
        Rational Design of Aquatic Treatment Systems.  Presented at the

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        American Society of Civil Engineers Spring Convention, Portland/ OR,
        April 14-18, 1980.  59 pp.

        The term "aquatic treatment" is defined as wastewater treatment in
     either natural or man-made wetlands.  A summary of the mechanisms in-
     volved in the removal of wastewater contaminants (including BOD, solids,
     N, P, heavy metals, refractory organic compounds, and bacteria and
     viruses) is included in the paper.  Effective use of charts and tables
     make the concepts easy to follow.  The following aquatic system design
     concepts are presented in the paper:

        0 Aquatic Processing Unit (APU): an assemblage of plants (and possibly
          animals) grouped together to achieve specific wastewater treatment
          objectives

        0 Aquatic plants and animals for use in aquatic treatment systems

        0 Climatic influences

        0 Environmental factors

        0 Wastewater characteristics

        0 Process reliability, upsets and recovery

        0 Resources recovery

        The authors also present a guide and checklist for the design of
     aquatic systems.  They conclude that more information is needed to
     establish fully rational design criteria for aquatic treatment systems.
     A bibliography of recommended literature also is included.  (EP)
864. Strange, J. R.  1976.  Effects of High Levels of Inorganic Phosphate on
        Aquatic Organisms in Phosphate-Rich Environments.  P. B. No. 263-390.
        Environmental Resources Center.  Georgia Institute of Technology.
        Atlanta, GA.
865. Straub, P., and D.M. Post.  1977. Rates of Growth and Nutrient Concentra-
        tion of Trees in Cypress Domes.  Pages 271-318. In; H.T. Odum and K.C.
        Ewel (eds.), Cypress Wetlands for Water Management, Recycling and Con-
        servation.  45th Annual Report to the National Science Foundation.
866. Struble, J. E.  1977.  Nitrification, Denitrification, and Sedimentatic
        Rates in Cypress Ponds Receiving Secondary Treated Sewage Effluent.
        Master's Thesis.  Dept. of Soil Science, Univ. of Florida, Gaines-
        ville- QT r*.

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867. Suffern, J. S., M. Adams, B. G. Blalock, C. C. Coutant, and C. A.
        Guthrie.  1978.  Growth of Monosex Hybrid Tilapia in the Laboratory and
        Sewage Oxidation Ponds. Presented at meetings of the World Mariculture
        Society, Atlanta, GA, 3 Jan. 1978.  18 pp.

        Studies were conducted to evaluate the potential of monosex hybrid
     Tilapia (female T. mossambica x male T. hornorum) in waste-heat polycul-
     ture systems.  The optimum growth temperature for this hybrid was found
     to be 32°C in laboratory experiments.  Experiments in sewage pond cage
     culture in the temperature range of 23 to 33°C at stocking densities of
     approximately 53 fish/m3 were also conducted. At fish sizes between 5
     and 12 cm TL, estimated annual production is approximately 50,000
     kg/ha/yr (50,000 lb/acre/ yr).  Fish in the sewage oxidation ponds grew
     signficantly faster than fish fed trout chow at optimum temperature in the
     laboratory, even though temperatures in the sewage ponds averaged below
     the optimum growth temperature.  Techniques to accelerate growth rates are
     being explored.  Exposure to gamma radiation (500 rads), known to cause
     significant increases in channel catfish growth rate, was found to have a
     similar effect on Tilapia.  After a 20-week growth period, exposed fish
     weighted an average of 20% more than controls.  (NT)
868. Suffern, J.S., C.M. Fitzgerald, and A.T. Szluha.  1981.  Trace Metal Con-
        centrations in Oxidation Ponds.  J. Water Pollut. Cont. Fed. 53,1599

        Heavy metal concentrations of Cd, Cr, Cu, Ni, Pb, and Zn in the waste-
     water, sludge, and biotic components of the Oak Ridge National Laboratory
     oxidation ponds were examined to determine whether metals accumulated in
     Tilapia.  Results indicated that metal levels in the wastewater and biotic
     components are generally low and that the major metal reservoir is the
     sludge.  Metals did not accumulate beyond standards in the muscle or liver
     °f Tilapia grown in the oxidation ponds.  This result may be partially due
     to the rapid growth rates of these fish (1-2 g fish~^ day~^), with new
     tissue developing more rapidly than metals can accumulate.  Another factor
     may be that the high concentrations of organic complexes in the ponds
     lower the availability of metals to the biota.  (AA)
869. Sullivan, R., J. T. Tierney, and E. P. Larkin.  1976.  Persistence of
        Poliovirus 1 in Soil Irrigated with Inoculated Sewage Sludge and
        Effluent, pp. 192-.  In The Abstract of the Annual Meeting of the
        American Society of Microbiologists.
870. Surakka, Seppo.  1971.  Some Observations on the Possibilities to Infil-
        trate Wastewater in Peatland According to Results Obtained at Kesaladen
        Town, Finland.  Vesitalous 3(5):26-31.  (Finnish-English Summary).
871. Surakka, S., and A. Kamppi.  1971.  Infiltration of Wastewater into Peat
        Soils.  SUO 22:51-57.  (Finnish with English Summary)

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872. Sutherland, J. C.  1977.  Investigation of the Feasibility of Tertiary
        Treatment of Municipal Wastewater Stabilization Pond Effluent Using
        River Wetlands in Michigan.  Final Report to the National Science
        Foundation, William and Works, Inc., Grand Rapids, MI. 171 pp.   (NTIS
        PB 275 283)

        The engineering feasibility of using Michigan river wetlands for
     tertiary treatment of municipal wastewater stabilization pond effluent
     was examined in relation to the alternatives of upland spray irrigation
     and chemical tertiary treatment.  Seventy-five Southern Peninsula rural
     municipalities with populations ranging from 360 to 2,800, served by
     treatment ponds, and with proximal streams, were studied by means of
     aerial reconnaissance.  Of these, 35%-40% were estimated to have a  viable
     wetland alternative for tertiary treatment.  The wetland method is
     estimated to be the most cost-effective at pond-wetland distances less
     than 4 miles.  The anticipated low or zero land cost and minimum of
     on-site hardware are favorable capital cost advantages.  Operational
     energy consumption would be minimized by low site pressure-head require-
     ments and the generally low-lying position of river wetlands.  The
     average potential capital cost savings in use of wetlands would be
     approximately $320,000 (1977 dollars) relative to upland irrigation, and
     approximately $150,000 relative to chemical treatment.  (AA)
873. Sutherland, J. C.  1979a. The Vermontville, Michigan, Wastewater-grown
        Volunteer Seepage Wetlands: Water Quality and Engineering Implica-
        tions.  In J.C. Sutherland and R. H. Kadlec  (eds.), Wetland Utiliza-
        tion for Management of Community Wastewater.  Abstracts of a confer-
        ence held 10-12 July 1979 at Higgins Lake, MI.

        The municipal wastewater treatment system at rural Vermontvilla, Michi-
     gan (pop. 975), consists of two faculative stabilization ponds (10.9 ac
     area), followed by four flood irrigation fields (11.5 ac area).  The
     fields are nearly overgrown with volunteer emergent aquatic vegetation,
     mainly cattail (Typha spp.).  Wastewater (0.068 MGD average inflow) is
     released from the ponds into the fields between June and November.  Low
     field berms contain the water until it infiltrates and percolates to the
     groundwater at 15—25 ft. depths.  Minor surface overflow sometimes occurs.
     No cutting or removal of vegetation has been done in the six-season his-
     tory of the fields.
        The wetland appears to add nitrogen species and phosphorus to the irri-
     gated water.  Anaerobic decomposition (thin organic soil mat) and release
     of nutrients by the standing crop are likely causes of the increases in
     these nutrients.  Greater than 97% of the P released from the stabiliza-
     tion ponds is removed in the soils beneath the fields before the perco-
     lating effluent reaches the ground water body.
        Operation and maintenance activities at Vermontville include only mow-
     ing of grassed wetland berms, manual valve control (8 hr/wk during 5-month
     irrigation season) and water quality monitoring. Wetland field maintenance
     seems not to be needed.  Capital costs for volunteer seepage wetland sys-
     tems, compared to upland spray irrigation, would be rougly 92% (0.5 MGD),
     75% (0.1 MGD), and less at still lower flows.
        Any deliberate construction of volunteer seepage wetlands for waste-
     water treatment in Michigan should be done with overflow prevention in

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     mind because of strict P effluent limitations.  Such construction could be
     technically feasible on fine-grained but noncohesive soils (hydraulic con-
     ductivity 10~3 - 10~4 cm/sec) which can be selectively compacted during
     construction.  (AA)
874. Sutherland, J.C. 1979b.  A Wetland Wastewater Feasibility Determination
        Procedure and Related Subjects for Discussion and Research.  Presented
        at the American Water Works Association Research Foundation, Water
        Reuse Symposium held 25-30 March 1979 in Washington, DC.  9 pp. plus
        supplement.

        Determination of feasibility involves at least ten major categories of
     consideration:  1) Wastewater treatment needs (wastewater quality, efflu-
     ent limitations, required removals, and wastewater volume.  2) Wetland
     distance.  3) Wetland ownership.  4) Wetlands protection legislation
     (federal, state, and legislation with regard to particular wetlands).  5)
     Wetland factors in wastewater treatment (modes of water exit from the wet-
     land, residence time, vegetation uptake).  6) Special wetland characteris-
     tics and relationships (world-reference values, regional and local values,
     needs which wastewater application might satisfy). 7) Wetland adequacy for
     wastewater treatment (with unaltered natural setting, with altered natural
     setting, degree of certainty).  8) Environmental impact (upon the wetland,
     after the wetland).  9)  Preliminary design.  10) Cost-effectiveness.
     The categories should be considered in the order given, but there should
     be savings in time if categories 2-4, 5-6, and 7-10 are addressed concur-
     rently.  In preparing the above outline, the following questions occur:
     1) Could wastewater grown wetlands be beneficially used as sanctuaries for
        rare, sensitive, or endangered plant or animal species?
     2) What are the ecological concerns vis-a-vis the winter application of
        wastewater to seepage wetlands under temperate climate conditions?
     3) How much wastewater (inches per week) should be added to a wetland?
     4) Are there any classifications (e.g., USFWS-Cowardin, et al.) of wetland
        that should be excluded a priori from wastewater application?
     5) Are the transformation and removal of nitrogen in wetlands well enough
        understood that a compilation or "systematics" on the subject from the
        existing literature would serve in lieu of detailed field studies as a
        basis of design for wastewater treatment/disposal?
     6) Are the phosphorus removal/immobilization factors well enough under-
        stood in flow-through wetlands that existing knowledge would suffice,
        in place of detailed stuldies, as a basis of design?
     7) How should new volunteer wetlands which exist solely because of waste-
        water application be managed?  (AA)
875. Sutherland, J.C.  1981.  Economic Implications of Using Wetlands for
        VJastewater Treatment, pp. 295-306. In; B. Richardson  (ed.), Selected
        Proceedings of the Midwest Conference on Wetland Values and Management.
        Minnesota Water Planning Board.  St. Paul.  660 pp.

        The cost of using Michigan riverine wetlands for  (hypothetical) treat-
     ment of pond-stabilization municipal wastewaters from small, mostly rural
     populations (range of flows, 0.038 - 0.35 MGD) were approximated in 1976-

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      1977,  and  are  herein updated to Spring,  1981  (ENR construction cost
      index).  The capital costs (C,  in $1,000's)  depend strongly upon distance
      from ponds to  wetland (D,  in miles),  with C = 117D + 262.  Forceline costs
      dominate in this  relationship.   Wastewater application would be at hydrau-
      lic  loadings of  less than  1  in/wk in all instances.
        The Houghton Lake Sewer Authority (Michigan)  recently added a natural
      state-owned peatland tertiary treatment system to its stabilization pond-
      seepage pond facilities  which serve approximately 6,000 full-time and
      14,000 seasonal population equivalents.   Similar updating of actual capi-
      tal  costs  (January,  1978 to  Spring, 1981 ) yields the approximate figure of
      $507,000,  excluding  engineering related costs, for the peatland system.
      There  are  not  costs  for  the  use of land.
        The 1980 wetland  operation and maintenance costs borne by HLSA totaled
      approximately  $19,000,  including labor plus overhead (16%), pumping-
      electrical (16%), administration (8%), vehicle use (3%), repair-replace-
      ment (0.5%), lab  equipment (3.5%), and state-required wildlife and vegeta-
      tion studies  (53%).
        Before  the  HLSA peatland  system concept was approved and funding
      obtained  (Municipal  Construction Grants Program), six years of scientific
      investigation  (University  of Michigan Wetlands Ecosystem Research Group)
      and  hundreds of person-hours in coordination efforts (HLSA, Williams &
      Works) were invested at  costs of $1 million to $2 million.  These costs,
      compared to projected capital savings of $800,000 relative to the upland
      irrigation alternative,  were borne by Michigan and U.S. taxpayers.  Al-
      though experience with natural wetland treatment systems may lead to
      reduction  in  documentation time and monetary costs in future situations,
      significant documentation  effort should, nonetheless, be anticipated
      because of the perceived values and individuality of natural wetlands.
        Operation and  maintenance costs related to the volunteer wetland-
      dominated  soil seepage system that treats pond-stabilized wastewater from
      the  Village of Vermontville, Michigan (pop. 900) were $4,800 annually,  of
      which  43%  are  for environmental monitoring.  (AA)
1092.  Sutherland,  J.C.   In press.
 876.  Sutherland,  J.C.,  and F.  Bevis.  1979. Reuse of Municipal Wastewater by
         Volunteer Freshwater Wetlands, pp. 762-782.  In; American Water Re-
         sources Association, Proceedings of the Water Reuse Symposium held
         25-30 March 1979 at Washington,  DC.  Vol. 1. Denver, CO.

         The volunteer wetland vegetation-organic mat complex at Vermontville
      actually adds phosphorus  to wastewater during the late spring and summer
      irrigation season.  Consequently, the wetland waters overflowing at the
      final discharge contain phosphorus in slight excess of permitted levels,
      absent a significant amount of diluting wastewater which has previously
      been filtered through the inorganic soils.  Phosphorus removal adequate
      to satisfy strict effluent requirements is accomplished only by seepage
      from the wetlands  through the inorganic soil substrate.
         No field maintenance has been required at Vermontville. There are no
      bare soil (tilled) areas  to be plugged through siltation caused by rain
      splash, spray irrigation, or flood-suspension of inorganic soils.  The
      Vermontville wetlands have shown buildup of three or four inches (0.1 m)
      of organic residues largely in the form of cattail straw after six irri-
      gation seasons. Over the 20-year design period the net accumulation would
      likely be less than 1 ft (0.3 m) due to progressive compaction and further

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     decomposition of older plant  residues.   Tree  control  is  not  needed at Ver-
     montville, although one of the fields may  soon be  overgrown  with willow
     whips.  On fields which might depend upon  a thin compacted soil  zone or
     other seepage restricting improvement,  tree control might  be needed to
     maintain the integrity of the conditioned  soil zone.
        The uptake of phosphorus by the prevailing cattail  standing crop is
     approximately 20% of the phosphorus released  into  the  wetlands.   Cattail
     harvesting would therefore be a means of reducing  effluent phosphorus.  But
     harvesting is not needed for  phosphorus removal in seepage wetland set-
     tings where subsurface soil types and volumes are  adequate to effect
     streams.
        Casual observation in 1978 reveals the  wastewater-grown wetlands have
     significantly added to the acreage of suitable, adequately isolated habi-
     tat for waterfowl and other wildlife in the Vermontville area.
877. Sutherland, J. C., and R. H. Kadlec  (eds.).   1979.  Wetland Utilization
        for Management of Community Wastewater.  Abstracts  of  a conference.
        Held 10-12 July 1979 at Higgins Lake, MI.  31 pp.

        This document is a collection of  24 abstracts  of presentations on
     wetland treatment systems in the United States and Canada given  at the
     conference.  Descriptions of a number of the  wetland treatment sites are
     given in Appendix B of this report.  (EP)
878. Sutton, D. L., and R. D. Blackburn.   1971.  Uptake  of  Copper  by Water
        Hyacinth.  Hyacinth Control J. 9:18-20.
879. Sutton, D. L, and W. H. Ornes.   1975.  Phosphorus  Removal  from Static
        Sewage Effluent Using Duckweed.  J. Environ. Qual. 4(3):367-370.

        Two experiments were conducted in containers to evaluate  the influence
     of various concentrations of static sewage effluent on  the growth  of  duck-
     weed and to determine the rate of P removal and harvesting effects on
     growth rates.  Several replicates were provided to ensure  data validity.
     Maximum growth rates were achieved at 25% effluent plus 75%  pond water.
     Increased effluent concentration did not increase  the growth rate  but did
     affect an increase in P uptake and protein content of plant  tissues.  There
     is a positive correlation between P concentration  in the water and duck-
     weed uptake rate up to 2.1 microgram ml.  With continued nutrient  input,
     half of the duckweed could be harvested every 4 days.   (AA)
880. Sutton, D. L., and W. H. Ornes.  1977.  Growth of Spirodela pqlyrhiza
        Static Sewage Effluent.  Aquatic Botany 3:231.

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881. Swett, D.  1979.  A Water Hyacinth Advanced Wastewater Treatment  System,
        pp. 233-255.  In; R.K. Bastian and S.C. Reed  (eds.)/ Aquaculture  Sys-
        tems for Wastewater Treatment: Seminar Proceedings and Engineering
        Assessment.  EPA 430/9-80-006. U.S. Environmental Protection Agency,
        Office of Water Program Operations, Municipal Construction Division,
        Washington, DC.

        A one year field experiment,  in Coral Springs, Florida,  (pop.  30,000),
     at the 378,530 Ipd (100,000 gpd) level has demonstrated that a system of
     5,035 m2 (54,200 ft2) of water hyacinth  (E ichhorni a cT;assiges) lagoons
     can provide advanced treatment to effluent from an activated sludge  plant
     by achieving removal rates of 67% for total suspended solids, 98% for bio-
     chemical oxygen demand, 97% for  total N and 79% for total P.  Biomass pro-
     duced by the system offers a potential for energy, fertilizer or  fodder
     production.  (AA)
882. Szczepanska, W., and A. Szczepanski.   1973.  Emergent Macrophytes and
        Their Role in Wetland Ecosystems.   Polskie Archiwum Hydrobiologgi
        20(1):41-50.
883. Tate, R. L. Ill, and R. E. Terry.   1980.  Effect of Sewage Effluent  on
        Microbial Activities and Coliform Populations of Pahokee Muck.  J.
        Environ. Qual. 9(4):673-677.

        The effect of sewage effluent was measured on microbial activity  in
     Pahokee muck.  Test plots were amended at weekly intervals with  sewage
     effluent at a rate of  5 cm/wk.  In  general, the indigenous microbial
     activity of the effluent-amended soil was greater than that of the soil
     which was water-amended or untreated.  An increase in indigenous activity
     occurred with the addition of the sewage effluent to the soil, and then
     declined steadily throughout the week between sewage additions.  The
     increase in activity immediately after amendment reflected the bio-
     chemical oxygen demand of the effluent.  Anaerobic bacterial populations
     did not differ significantly between the sewage-effluent or water-amended
     samples or unamended soil plots.  However, significant variations were
     noted for various sample dates, most likely resulting from soil  moisture
     changes.  The augmented dehydrogenase activities and generally elevated
     carbon metabolic rates in amended soils indicate that microbial  activity
     in the muck was stimulated.  Coliform bacteria passed through the soil
     profile, but the increased moisture, not the nutrients, of the sewage
     effluent stimulated the oxidation of soil organic matter, thereby
     stimulating soil subsidence.  Therefore, a sewage disposal plan  involving
     cultivated organic soils must be viewed as a threat to the longevity of
     the soils.  (WR)
884. Tchobanoglous, G.  1979.  Wetland Systems for Wastewater Treatment in
        Cold Climates: An Engineering Assessment.  Report to U.S. Army Cold
        Regions Research and Engineering Laboratory, Hanover, NH.  43 pp.

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885. Tchobanoglous,  G., J. Colt, and R. Crites.  1979.  Energy and Resource
        Consumption in Land and Aquatic Treatment Systems.  Presented at the
        Energy Optimization of Water and Wastewater Management for Municipal
        and Industrial Applications Conference, Department of Energy, New
        Orleans, LA, 10-13 December, 1979. 12 pp.
886.  Tchobanoglous, G., and G. L. Gulp.  1980.  Wetland systems for wastewater
        treatment: an engineering assessment, pp. 13-42.  In S. C. Reed, and
        R. K. Bastian (eds.), Aquaculture Systems for Wastewater Treatment: An
        Engineering Assessment.  EPA  430/9-80-007.  U.S. Environmental Pro-
        tection Agency, Office of Water Program Operations, Municipal Con-
        struction Division, Washington, DC.

        The use of natural and artificial wetlands for the treatment of
     wastewater is examined in this engineering assessment.  The primary
     objective of the assessment was to answer the question of whether the
     technology of using wetlands for the treatment of wastewater  is ready  for
     routine use and, if not, what must be done to make it a reality.  On the
     basis of 1) treatment efficiency and reliability, 2) availability of
     design criteria and procedures, 3) availability of proven managment
     techniques, 4) energy and resource consumption, 5) costs, and 6) health
     risks, it is concluded that the current status of wetlands technology  is
     not yet developed to the point where the use of wetland systems can be
     considered routine.  Data and information that must be developed before
     the design of wetland systems can become a rational undertaking are
     identified and discussed. (AA)
887. Tchobanoglous, G., R. Stowell, R. Ludwig, J. Colt,  and A. Knight.   1979.
        The Use of Aquatic Plants and Animals for the Treatment  of Wastewater:
        an Overview, pp. 35-55.  In S. C. Reed, and R. K. Bastian  (eds.),
        Aquaculture Systems for Wastewater Treatment: An Engineering Assess-
        ment.  EPA  430/9-80-006.  U.S. Environmental Protection Agency,
        Office of Water Program Operations, Municipal Construction Division,
        Washington, DC.

        Aquatic systems employing plants and animals have been proposed as
     alternatives to conventional wastewater treatment systems.  The fun-
     damental difference between conventional and aquatic systems  is that  in
     the former, wastewater is treated rapidly in highly managed environments,
     whereas in the latter, treatment occurs at a comparatively  slow rate  in
     essentially unmanaged natural environments.  The consequences  of this
     difference are 1) conventional systems require more construction and
     mechanization but less land than aquatic systems, and 2) conventional
     processes are subject to  greater operational control and less  environ-
     mental influence than aquatic processes.  The major stimulus  for further
     research into the fundamentals, design, and management  of aquatic systems
     is the potential for reducing the construction, operation,  and  mainten-
     ance costs for wastewater treatment.  The general concepts  involved in
     the design and use of aquatic systems are presented and the implications
     are discussed in this overview.  (AA)

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888. Teal, J.M. and I. Valiela.  1973.  The Salt Marsh as a Living Filter.
        Mar. Tech. Soc. Jour., 7: 19-21.
889. Teal, J. M., and I. Valiela.  1975.  Enrichment of Coastal Wetlands.
        Woods Hole Oceanographic Institute.  Woods Hole, MA.

        Nutrient enrichment of coastal wetlands enhances production of both
     plant and animal components.  Nitrogen accounts for most of the effect.
     Pollutants tend to be bound on sediments but are also taken up by
     organisms in proportion to their mobility.
890. Tenney, M. W., W. F. Echleberger, Jr., K. J. Guter, and J. B. Carberry.
        1972.  Nutrient Removal from Wastewater by Biological Treatment
        Methods, pp. 391-421.  In H. E. Allen and J. E. Krammer (eds.),
        Nutrients in Natural Waters.  John Wiley and Sons, Inc., NY.
891. Teskey, R. O., and T. M. Hinckley.  1977a. Impact of Water Level Changes
        on Woody Riparian and Wetland Communities.  Vol. I: Plant and Soil
        Responses to Flooding.  FWS/OBS-77/58.  U. S. Fish and Wildlife Ser-
        vice.  30 pp.

        Volume I is a scientific literature review detailing the impact of
     water level changes on growth and development of important riparian and
     wetland trees and shrubs and describing physiological mechanisms.  It  is
     applicable to all geographical areas in the contiguous 48 states.
892. Teskey, R. O., and T. M. Hinckley.   1977b. Impact of Water Level Changes
        on Woody Riparian and Wetland Communities.  Vol. II: The Southern
        Forest Region.  FWS/OBS-77/59.  U. S. Fish  and Wildlife Service.
        30 pp.
893. Teskey, R. O., and T. M. Hinckley.   1977c. Impact  of Water Level Changes
        on Woody Riparian and Wetland Communities.  Vol. Ill: The Central
        Forest Region.  FWS/OBS-77/60.  U.S. Fish  and Wildlife Service.
        36 pp.
894. Teskey, R. O., and T. M. Hinckley.   1978a.  Impact  of Water Level Changes
        on Woody Riparian and Wetland Communities.  Vol. IV: Eastern Deciduous
        Forest Region.  FWS/OBS-78/87.  U.S. Fish and Wildlife Service.
        36 pp.

        This volume of the series covers  the important  woody plant  species  in
     the Eastern Deciduous Forest Region, which  encompasses the area from
     southern Minnesota eastward to  the Appalachians.

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895. Teskey, R. 0., and T. M. Hinckley.  1978b. Impact of Water Level Changes
        on Woody Riparian and Wetland Communities.  Vol. V: Northern Forest
        Region.  FWS/OBS-78/88.  U.S. Fish and Wildlife Service.  54 pp.

        This volume of the series covers the important woody plant species in
     the Northern Forest Region, which encompasses the Great Lakes region from
     Minnesota eastward to the Atlantic Ocean.
896. Teskey, R. O., and T. M. Hinckley.  1978c. Impact of Water Level Changes
        on Woody Riparian and Wetland Communities.  Vol. VI: Plains Grassland
        Region.  FWS/OBS-78/89.  U.S. Fish and Wildlife Service.  54 pp.

        This volume of the series covers the important woody plant species in
     the Plains Grassland Region, which covers the middle west of the United
     States from eastern Montana to western Minnesota and south to Texas.
897. Teskey, R. O., and T. M. Hinckley.  1980.  Impact of Water Level Changes
        on Woody Riparian and Wetland Communities.  Vol. VII: Mediterranean
        Region, Western Arid and Semi Arid Region.  FWS/OBS-78/93.  U.S. Fish
        and Wildlife Service.  54 pp.
898. Texas Dept. of Health.  1976.  Water Hyacinth Culture for Wastewater
        Treatment.  Austin, TX.
899. Thomson, S.V. and R.M. Allen.  1974.  Occurrence of Phytpphthora species
        and Other Potential Plant Pathogens in Recycled Irrigation Water.
        Plant Dis. Rep. 58:945-949.
900. Thorhaug, A.  1977.  Ecological Study of the Effects of Power Plants on
        Benthic Macroplant Microcosms in Subtropical and Tropical Estuaries.
        Progress Report, 1976-77.  Miami Univ., FL. 34 pp.

        The effect of two fossil fuel and two nuclear plants on a near-shore
     seagrass community was studied in South Biscayne Bay,  Dade County,
     Florida, over the past four years.  The focus of the study was to
     delineate the effects that a series of various pollutants have had on the
     dominant system of seagrass Thalassia testudinum and its related com-
     munity.  Data are included from studies on the effects of heavy metals,
     waste heat, and dredging activities, with emphasis on thermal pollution.
     In order to separate the perturbation of the specific pollutant from the
     natural fluctuations of the system, efforts were made to understand the
     dynamic processes in adjacent untouched areas.  (NT)

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 901. Tietjen, W. L.,  and  J.  C.  Carter.   1980.   Phosphate Uptake by River Swamp
        Ecosystems.   Georgia Southwestern College.   Americus,  GA.
902. Tiffin, L. O.   1977.  The  Form and Distribution of  Metals in Plants:  an
        Overview, pp.  315-334.   In  H.  Drucker,  and R.  E.  Wildung (eds.),
        Biological Implications of  Metals  in the Environment.   ERDA Symposium
        Series No. 42.  Energy  Research and Development  Administration, Wash-
        ington, DC.
903. Tilton, D. L.   1976.  Recovery  of Nutrients  from Peatlands  used for
        Tertiary Treatment, pp.  307-327.   Iri  D. L.  Tilton,  R.  H.  Kadlec, and
        C. J. Richardson  (eds.). Proceedings  of a National  Symposium on Fresh-
        water Wetlands and Sewage Effluent Disposal.   Univ.  of Michigan, Ann
        Arbor.

        The potential for wetlands to simultaneously  act  as  tertiary treatment
     systems and produce a harvestable crop is considered.   Characteristics  of
     organic soils which are  important to  their use as tertiary  treatment al-
     ternatives and which influence  crop production are discussed.  In addition,
     current and potential uses  of wetlands are described,  and the  compatibil-
     ity of each use with tertiary treatment  is evaluated.
        Analysis of the Porter Ranch Peatland project (Michigan  project) indi-
     cates that vegetable and/or field crop production is not  feasible, but
     silviculture of appropriate pulpwood  species may be possible.   Forestry
     provides advantages in harvesting, nutrient  storage, and  low risk due to
     heavy metals and viruses.  However, forestry may not be feasible if water-
     fowl production is a management goal  for the area or if nutrient require-
     ments of the trees are not met  by nutrient additions in the  effluent.
904. Tilton, D. L.  1977a. The Effects of Secondary Effluent on the Plant and
        Soil Components of a Northern Wetland Ecosystem.  In R. H. Kadlec, D.
        L. Tilton, and J. A. Kadlec  (eds.), Feasibility of Utilization of
        Wetland Ecosystems for Nutrient Removal from Secondary Municipal
        Wastewater Treatment Plant Effluent.  Semi-annual Rept. No. 5.  Univ.
        of Michigan, Ann Arbor.

        This paper describes the effect of discharging 12 million gallons of
     secondarily treated effluent on the growth of vascular plant species.
     The nutrient content of certain ecosystem components is described.  (WE)
905. Tilton, D. L.  1977b. Nitrogen Dynamics in Northern Freshwater Wetlands.
        Bulletin of the Ecological Society of America.
906. Tilton, D.L.  1978.  Wastewater Treatment via Wetland Irrigation: Nutrient
        Dynamics, pp. 178-197. In; C.B. DeWitt and E. Soloway (eds.), Wetlands:

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        Ecology, Values/ and Impacts.  Proceedings of the Waubesa Conference on
        Wetlands, 2-5 June, 1977.  Madison, WI.  Institute for Environmental
        Studies, Univ. of Wisconsin, Madison.

        The effect of discharging approximately 10 million gallons of secondary
     effluent onto a ten acre section of wetland is described.  Total dissolved
     P, NH4 - N, N(>3 + NO2 - N, and Cl were measured in the effluent and in
     surface waters various distances (30-255 m) from the discharge location.
     Total dissolved P and NO3 + NC>2 - N in surface waters had returned to
     background concentrations within 30 m of the discharge, while Cl tended
     not to decrease with distance as much as the more biologically active
     ions.  Element budgets showed the 99 percent, 95 percent and 71 percent of
     the total input of nitrate+nitrite-N, total dissolved P, and NIfy - N,
     respectively, were immobilized within 30 m of the discharge site.  Element
     immobilization occurred by a combination of physical and biological pro-
     cesses.
907. Tilton, D.L.  1981.  The Environmental Impacts Associated with Develop-
        ments in Wetlands, pp. 357-362.  In; B. Richardson (ed.), Selected Pro-
        ceedings of the Midwest Conference on Wetland Values and Management.
        Minnesota Water Planning Board. St. Paul.  660 pp.

        The environmental impacts associated with the utilization of wetland
     ecosystems for stormwater management, wastewater treatment, or other pur-
     poses is not widely understood.  This paper suggests a conceptual frame-
     work which is useful for the preparation of wetland environmental impact
     assessments and presents several examples of impacts associated with
     altering the energy budget, nutrient budget, or hydrologic budget of
     various wetland ecosystems.
908. Tilton, D.L. and R.H. Kadlec.  1979.  The Utilization of a Freshwater Wet-
        land for Nutrient Removal from Secondarily Treated Wastewater Effluent.
        J. Environ. Qual. 8(3):328-334.

        To test the feasibility of utilizing a fresh-water wetland for tertiary
     wastewater treatment, secondarily treated wastewater was applied to a wet-
     land in the northern lower peninsula of Michigan.  During the discharge of
     38,840 m3 (10,260,000 gal) and 23,520 m3 (6,213,000 gal) of secondar-
     ily treated wastewater in 1976 and 1977, respectively, surface water qual-
     ity, plant productivity, and nutrient status of plants and soils were
     measured.  In 1976 the concentration of (nitrate + nitrite)-N averaged
     0.36 mg/1 in the effluent and 0.01 mg/1 at stations 25 m from the dis-
     charge pipeline.  Total dissolved P (TDP) averaged 0.41 mg/1 in the
     effluent and 0.11 mg/1 at sampling stations 25 m from the discharge.  The
     efficiency of P removal from the effluent was greater when surface water
     depth were 6 cm compared to 30 cm.  Ammonium - N concentrations were
     rarely higher in the effluent compared to background concentrations in the
     wetland surface waters.  In 1977 a point source distribution system was
     used.  The effluent averaged 1.50 mg/1 nitrate - N and 1.57 mg/1 TDP.
     Within 30 m, nitrate - N was  0.10 mg/1 in the surface water and within
     80 m TDP was 0.07 mg/1.  Biomass of live plants in 1976 was somewhat

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     greater near the pipeline compared to control areas.  Leaves of cattail
     (Typha latifolia) were longer near the pipeline in 1976 compared to control
     areas.  Chemical analysis of sedge (Carex spja.) leaves and roots, standing
     dead and litter sampled in 1976 showed no significant differences in N
     concentrations among sampling locations, but P concentrations were higher
     in leaves and roots 6 m from the pipeline compared to control areas.  (BA)
909. Tilton, D. L., R. H. Kadlec, and J. Linde.  1977.  Surface Water
        Chemistry and Element Budgets of a Wetland Used for Wastewater Treat-
        ment , pp. 53-126.  In R. H. Kadlec, D. L. Tilton, and J. A. Kadlec
        (eds.), Feasibility of Utilization of Wetland Ecosystems for Nutrient
        Removal from Secondary Municipal Wastewater Treatment Plant Effluent.
        Semi-Annual Report No. 5.  Univ. of Michigan, Ann Arbor.

        The effect of discharging approximately 10 million gallons of secondary
     effluent onto a ten acre section of a northern Michigan wetlands is des-
     cribed.  Total dissolved P, NH4-N, NO3+NO2-N, Cl, Na, Ca, Mg, K, Ni, Zn, Cu,
     Pb, COD, pH, alkalinity, hardness, and specific conductivity were measured
     in the effluent and in surface waters various distances (30 - 225 m) from
     the discharge locations.  Total dissolved P, N03+NO2-N, and pH in surface
     waters had returned to background concentrations within 30 m of the dis-
     charge.  Alkalinity and hardness decreased to background concentrations 50
     m from the discharge site, while Cl, Ca, Mg, Na, and to a lesser extent, K
     tended not to decrease with distance as much as the more biologically ac-
     tive ions.  COD was lower in the effluent than surface waters in the wet-
     land.
        Element budgets showed that 99%, 95% and 71% of the total input of
     nitrate+nitrite-N, total dissolved P, and NH4~N, respectively, were
     immobilized within 30 m of the discharge site.  Equivalent immobilizations
     of the total input for K, Mg, Na, Ca, and Cl were 69%, 42%, 39%, 32%, and
     30%, respectively.  Element immobilization occurred by a combination of
     physical and biological processes.  (AA)
910. Tilton, D. L., R. H. Kadlec, and C. J. Richardson (eds.).  1976.  Pro-
        ceedings of a National Symposium on Freshwater Wetlands and Sewage
        Effluent Disposal.  Univ. of Michigan, Ann Arbor. 343 pp.

        The symposium was held to discuss the feasibility of using freshwater
     wetland ecosystems to remove certain chemicals from secondarily treated
     wastewater before they enter lakes, streams, and groundwaters.  The
     following represents a general list of those areas of consideration: (1)
     Hydrology: fate of water beyond the wetlands, loading rate, flow rate
     within the wetland; (2) Nutrient Dynamics and Accumulation: efficiency of
     treatment, mechanism of nutrient removal from wastewater, loading rate;
     (3) Environmental Impacts: changes in species composition, habitat pro-
     tection, health; (4) Legal Aspects: enabling policy and legislation; (5)
     Economic Considerations: savings in construction cost, benefits associ-
     ated with protection of environmental quality; (6) Land Use: compatible
     with other traditional uses of wetlands, policies associated with state
     and federal wetlands; and (7) Design Considerations: artificial or
     natural wetlands, method of effluent discharge, schedule of pumping,
     determination of discharge rate.   (NT)

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911. Tilton, D.L., and B.R. Schwegler.  1978.  The Values of Wetland Habitat in
        the Great Lakes Basin,  pp. 267-277, In; P.E. Greeson, J.R. Clark, and
        J.E. Clark (eds.), Wetland Functions and Values:  The State of Our
        Understanding.  American Water Resources Association, Minneapolis, MN.
912. Tischer, R. G., and L. R. Brown.  1962.  Mechanism of Algae-Bacteria
        Complexes in Oxidation Ponds.  Progress Rept. Mississippi State Univ.,
        State College. 50 pp.

        During the course of the investigation it was found that practically
     all of the ponds tested contained some thermophilic micro-organisms.
     Studies conducted with these thermophiles indicated that they held con-
     siderable potential as a means of stabilizing domestic sewage.  It seemed
     desirable to include in the report only that material relating to sewage
     stabilization by aerobic thermophiles.  (NT)
913. Tortell, P.  The Utilization of Waste Nutrients for Aquaculture.  Pre-
        sented at IAWPR Developments in Land Methods of Wastewater Treatment
        and Utilization International Conference, held Oct. 23-27, 1978 in
        Melbourne, Australia.

        Fish pond fertilization with human and animal wastes has been prac-
     ticed for centuries.  Sewage effluent use for aquaculture is reviewed,
     and integrated wastewater-aquaculture systems are analyzed.  The need for
     large areas of land and adequate public health safeguards, and logistics
     of harvesting are problems that can be easily solved; consumer acceptance
     is more difficult to achieve.  Until this obstacle can be overcome, the
     products of wastewater-aquaculture (unicellular algae, aquatic macro-
     phytes, invertebrates, and finfish) can be put to other uses.  There is a
     consensus that aquaculture has great potential as an alternative to
     conventional waste treatment and disposal systems.  (EL)
914. Toth, L.  1972.  Reeds Control Eutrophication of Balaton Lake.  Journal
        Internat. Assoc. Water Pollution Res. 6(12):1533-1539.

        Wastewater was applied to the upland edge of a reed swamp on the
     border of Lake Balaton in Hungary.  Nutrients in the wastewater were
     removed effectively by passage through the swamp.  (EP)
915. Tortora, L.  1978.  Analysis of Phosphorus Data from Wetlands Application
        Site for Secondarily Treated Municipal Effluents.  Master's Paper.
        Dept. of Environmental Engineering Sciences, University of Florida,
        Gainesville.

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916. Tourbier, J., and R. W. Pierson, Jr. (eds.).  1976.  Biological Control
        of Water Pollution.  Univ. of Pennsylvania Press, Philadephia.

        This publication contains a number of articles that are related to the
     topic of wastewater renovation by natural and artificial wetlands, in-
     cluding research performed in foreign countries.  The section entitled
     "Biological Treatment of Wastewater" contains a number of papers that
     deal with specific vegetation types and their relationship to water
     purification.  (EP)
917. Trieff, N. M. et al. 1976.  Sewage Treatment by Controlled Eutrophication
        Using Algae and Artemia, pp. 231-239.  In Biological Control of Water
        Pollution.  Univ. of Pennsylvania, Philadelphia.
918. Trimberger, J.  1972.  Production of Fathead Minnows (Pimephales prqmelas)
        in a Municipal Wastewater Stabilization System.  Michigan Department of
        Natural Resources, Grand Rapids.  5 pp.

        Sewage oxidation ponds may provide a valuable culture media for
     rearing forage fish for piscivorous species—especially muskellunge.  The
     fish manager should consider using lagoons to rear forage fish for
     restocking lakes following chemical treatment.
919. Tsai, C.  1975.  Effects of Sewage Treatment Plant Effluents on Fish: a
        Review of the Literature.  Publication No. 36.  Chesapeake Research
        Consortium, Inc., Baltimore, MD.
920. Tsai, C.  1978.  Impact of Wastewater Treatment Plant Discharges,
        pp. 149-150.  In K. C. Flynn and W. T. Mason (eds.), The Freshwater
        Potomac - Aquatic Communities and Environmental Stresses.  Proceedings
        of a symposium held in January 1977 at College Park, MD.  Interstate
        Commission on the Potomac River Basin, Rockville, MD.
921. Turner, R. E., J. W. Day, Jr., M. Meo, P. M. Payonk, T. B. Ford, and W.
        G. Smith.  1976.  Aspects of Land-treated Waste Application in
        Louisiana Wetlands, pp. 145-168.  In D. L. Tilton, R. H. Kadlec, and
        C. J. Richardson (eds.), Proceedings of a National Symposium on
        Freshwater Wetlands and Sewage Effluent Disposal.  Univ. of Michigan,
        Ann Arbor.

        This is a summary of a short-term feasibility study utilizing a com-
     bination of overland flow and topical application of menhaden fisheries
     waste in a Louisiana freshwater, coastal marsh.  Significant amounts
     (40-50%) of C, N, and P were lost to the soil-plant system when waste-
     water followed an overland flow route.  Live standing crops were

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     significantly higher when wastes were applied directly.  Both application
     procedures increased net production of the three plant communities
     between 32-50%.  Less than 5% of total nutrient application was  taken  up
     by plants.  The soil-water interface is implicated as the major  site of
     sorption, deactivation, and denitrification for both application pro-
     cedures.  Authors suggest use of abundant spoil disposal sites for this
     type of treatment system.  (WE)
922. Tusack-Gilmour, D.  1980.  Effluent Creates an Oasis in Nevada.  Water
        and Wastes Engineering 17(9):22-24, 56.

        The Las Vegas Wash, fed by effluents from the Las Vegas and Clare
     County (Nevada) wastewater treatment plants, is a lush, 4,000-acre bird
     and animal retreat in the desert.  More than 200 species of birds and
     animals inhabit it, and marsh and riparian vegetation abounds.  However,
     excessive perennial flows and flooding during summer and winter have
     caused extensive damage; erosion and headcutting must be remedied to
     preserve the Wash.  Besides its present status as a wildlife habitat,
     several existing and proposed beneficial uses for the Wash include a
     recreation-park area, education and research, hunting, a flood flow
     conduit and urban stormwater treatment system, a wastewater effluent
     conduit, a potential treatment system for the removal of P, N, and other
     pollutants, a salinity control point for reducing TSS levels to the
     Colorado River system, and a source of return flow credits for expanding
     the water supply system.  Recent progress by concerned conservation
     groups and public agencies has strengthened the concept of maintaining
     and improving this unique wetlands environment.  Preservation of its
     distinctive features depends upon development of an overall marsh-wet-
     lands management plan and the support to implement it.  (PA)
923. U.S. Environmental Protection Agency.   1973.  Tertiary Treatment  with
        a Controlled Ecological System. Pub. No. EPA-660/2-73-022.  National
        Water Quality Lab. Duluth, MN.

        A two-stage pond system was constructed and operated as a process for
     polishing secondary sewage effluent.  In the shallow first pond a luxur-
     iant growth of algae was maintained.  In the second stage a population of
     Daphnia jjujLex effectively removed the algae.  Total volume of the system
     was 1,135 cubic meters.  A program of chemical and biological monitoring
     was followed over a twelve-month period.  Objectives were to demonstrate
     feasibility of the process for producing recreational-grade water, acquire
     operating data on a completely biological process, and determine  its po-
     tential for nutrient removal.  The system was operated with about 10 days'
     detention in each stage.
        The Daphnia remained as the dominant zooplankton species in the second
     stage pond throughout the observation period, and during periods  when
     their concentration was above 500 organisms/liter, were able to hold water
     transparence at Secchi disk readings around 2 meters.  At such times COD
     reduction was above 40 percent across the system.  Significant removal of
     nutrients occurred only during the months of July and August when nitrogen

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     and phosphate reductions were 48 percent and 63 percent,  respectively.
     Nutrient removal performance was hampered by occasional invasions  of
     Daphnia or rotifers in the first stage pond, which decimated  the algae
     population; such events were not successfully controlled  and  remain the
     principal obstacle to further development of the process.
924. U.S. Environmental Protection Agency.  1976a. Wastewater Treatment  by
        Natural and Artificial Marshes.  EPA Technology Transfer  Series
        EPA-600/2-76-207.
925. U.S. Environmental Protection Agency.   1976b. Economic Assessment  of
        Wastewater Aquaculture Treatment Systems.  Cincinnati, OH.  118  pp.

        A desk study of the economic viability of aquaculture  as  an alterna-
     tive to conventional wastewater treatment systems was undertaken on
     behalf of small townships in southwest U.S.A.  A total of 15 alternative
     treatment combinations was examined, in which aquaculture either in the
     form of a raceway or lagoon was adopted in  11 instances,  in  conjunction
     with various forms of preliminary biological treatment.   The most
     cost-effective strategy for meeting particular criteria,  such  as the
     degree of removal of BOD or dissolved nutrients, or the fulfillment of
     advanced water treatment standards, was selected. It was  demonstrated
     that in certain situations wastewater aquaculture presented  a  low-cost
     alternative to conventional treatment technology, but that,  on the basis
     of current knowledge, it may not provide the most economic method  of
     meeting stricter effluent standards.  (AL)
926. U.S. Environmental Protection Agency.   1980a. Mutagenic Activity  and Chem-
        ical Characterization for the Palo Alto Wastewater Reclamation and
        Groundwater Injection Facility. Pub. No. EPA-600/S1-81-029.  Washington,
        DC.

        At the Palo Alto Reclamation Plant 0.44 cu m/s  (1 mgd)  of  secondary
     effluent is reclaimed through a series  of wastewater treatment  processes.
     Mutagenic activity was consistently found to be present in the  secondary
     treated municipal wastewater influent to the Reclamation Facility.   This
     activity was not reduced significantly  by high time treatment,  air strip-
     ping, recarbonation, or ozonation, even though these processes  did remove
     a portion of the overall organic content of the wastewaters and many of
     the volatile organic compounds.  Activated-carbon  adsorption  was  effective
     in removing mutagenic activity to  such  a degree that mutagenic  activity
     could not be found in water used for injection or  that taken  from monitor-
     ing wells.  Chlorination resulted  in an increase in mutagenic activity.  A
     laboratory study demonstrated that this increase in activity  would not
     result if chlorine dioxide rather  than  chlorine were used  for disinfec-
     tion. Chlorination resulted in the production of a significant  concentra-
     tion of non-purgeable, but otherwise unidentified, chlorinated  organic
     compounds, as well as trihalomethanes.  Formation  or removal  of such
     compounds did not seen to correlate with changes in mutagenic activity.

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     Air stripping by the fountain-spray system was most effective in removing
     volatile organic compounds, while activated carbon was responsible for
     removals obtained for most other organic materials.
927.  U.S. Environmental Protection Agency.  1980b. Treatment of Primary Efflu-
        ent by Rapid Infiltration.  Pub. No. EPA-600/S2-80-207.  Washington, DC
928. U.S. Environmental Protection Agency.  1980c. Municipal Solid Waste
        Disposal in Estuaries and Coastal Marshlands EPA-600/S2-80-212.
929. U.S. Environmental Protection Agency.  1981a. Process Design Manual for
        Land Treatment of Municipal Wastewater.  EPA 625/1-81-013.
930. U.S. Environmental Protection Agency.   1981b. Sewage Sludge Viral and
        Pathogenic Agents in Soil-Plant Animal Systems.  (EPA-600/S1-81-026),
        Washington, DC.
931. U.S. Environmental Protection Agency.   1981c. Cropping Systems  for Treat-
        ment and Utilization of Municipal Wastewater and Sludge.  Pub. No.  EPA-
        600/S2-81-065.  Washington, DC.

        The efficiency of nitrogen stripping by  intercropping  forages  with  corn
     is evaluated in a three-year study at Muskegon County Wastewater  Treatment
     Facility in Michigan.  Corn is grown as the major  cash crop  at  the Muske-
     gon treatment facility, but experience  has  shown that corn is not effi-
     cient in removing nitrogen from applied wastewater except during  a few
     short weeks during its growing season.  Studies were designed to  evaluate
     the improved nitrogen removal for the entire  irrigation season  when  corn
     is no-till planted in a number of selected  forage  crops.  In addition  to
     nitrogen stripping studies, sludge compatibility and metal contaminated
     sludge studies were evaluated to determine  the feasibility of applying
     sludge to land which is being used in a wastewater treatment system.
932. U.S. Environmental Protection Agency.   1981d.  Evaluation  of  the Health
        Risks Associated with  the Treatment  and Disposal  of  Municipal Waste-
        water and Sludge.  Pub. No.  EPA-600/S1-81-030.  Washington,  DC.
933. U.S. Environmental Protection  Agency.   1981e.  Health Effects of Sewage
        Aerosols:  Additional  Serological  Surveys  and Search for Legionella
        pneumophila  in Sewage.   Pub.  No.  EPA-600/S1-81-032.  Washington, DC.

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        Antibody  levels  to  Legionella pneumophila,  sero-group 1 and Hepatitis A
     Virus  (HAV)  were  determined for 433 persons living within a 1.6 km radius
     of an  activated sludge plant.   Sera of children 6 to 13 years of age were
     also tested  for antibody  to Norwalk Virus.   The antibody prevalence rates
     for L. pneumophila,  HAV and Norwalk Virus were 23.2%, 31.55% and 7.1%,
     respectively.  The  prevalence  of L.  pneumophila antibody was higher than
     expected  but not  the others.  Seroconversions  were not observed except for
     a 4-fold  rise  in  HAV antibody  in one pair of sera suggesting new infec-
     tions  did not  occur in the 8-month study period by any of these agents.
     Using  an  index of exposure for the number of viable organisms generated at
     the plant to which  participants were exposed,  the average index for per-
     sons with antibody  to  L.  pneumophila or HAV, was not different from the
     averages  of  those without antibody.   In addition, there was no association
     between how  close people  lived to the plant, or how long they lived in the
     study  area,  and being  seropositive.   These  findings augment but still did
     not prove the  previous findings that sewage aerosols emitted from this
     plant  had no overt  health effects.
934. U.S. Environmental Protection  Agency.   1981f.  Health Risks of Human Expo-
        sure to Wastewater.   Pub. No.  EPA-600/S1-81-002.   Washington,  DC.

        In this study  started in  1977,  clinical  and serologic evaluations of
     workers involved  in  composting of  wastewater treatment plant sludge by the
     aerated pile method  was  initiated to  evaluate the potential health effects
     of exposure to Aspergillus fumigatus  and other viable and nonviable compo-
     nents of  sludge.  A  health study  consisting of analysis of blood specimens
     for liver and kidney function  parameters, determination of serum and urine
     bile acid concentrations, physical examinations,  interviews, and air and
     water monitoring  was conducted of  residents exposed  to carbon tetrachlor-
     ide and other toxic  chemicals  in  drinking water as a result of contamina-
     tion of domestic  wells by a  nearby hazardous waste dump.  A study was
     undertaken to determine  if evidence of  exposure to hexachlorocyclopenta-
     diene, hexachlorabicycloheptadiene, heptachlorobicycloheptane,  and chlor-
     dene could be detected by urine analysis of workers  exposed to chlorinated
     insecticide substances discharged  to  the sewer by a  pesticide formulator.
     In order to assess the potential  for  health risks associated with the
     spray irrigation  of  wastewater, a  clinical  and virus serologic evaluation
     of workers and wastewater and  air  monitoring for  viruses,  pathogenic bac-
     teria, and volatile  organic  chemicals was conducted.   A field ecosystem
     study was carried out to evaluate  the effects  of  land application of heat
     dried municipal wastewater treatment plant  sludge (Milorganite)  on vege-
     tation, insects and  medow voles.   The potential health effects associated
     with sludge incineration and pyrolysis  are  also presented.
935. U.S. Environmental Protection Agency.   1981g.  Helminth  and Heavy Metals
        Transmission from Anaerobically Digested Sewage  Sludge.   Pub.  No.  EPA-
        600/S2-81-024.  Washington, DC.

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 936. U.S. Environmental Protection Agency.  1981.  Soil Temperature and Sewage
         Sludge Effects on Plant and Soil Properties.  Pub. No. EPA-600/S2-81-
         069.  Washington, DC.

         A field experiment was conducted to determine the influence of soil
      temperature and sewage sludge on growth and composition of corn  (Zea mays
      L.).  Changes in soil organic matter, extractable metals, pH, bulb den-
      sity,  aggregation, fecal coliform, and fecal streptococcus were  deter-
      mined.  Soil temperatures studied were 16, 27, 35, and 22C°  (ambient).
      Sludge was applied at rates of 0, 56, and 112 metric tons per hectare.
      Corn yields and growth were reduced by 16C° soil temperature, and in-
      creased by heating the soil to 35C° in 1976, but the warmer  soil did not
      significantly increase yields in 1975.  The effects of soil  temperature on
      heavy metal concentrations in plants varied according to year, metal, and
      plant part.  Zinc concentrations generally increased with increased soil
      temperature.  Sludge increased concentrations of Zn, Cu, Ni, and Cd in
      corn,  radishes, legume and small grain tissues.  Cd increased significant-
      ly in corn seedlings with soil temperature increase, but not in  stover.
      The highest rate of fecal coliform and fecal streptococcus addition and
      the longest survival times in soil were on plots receiving the highest
      sludge application.  Survival time varied directly with soil temperature.


 937. U.S. Environmental Protection Agency.  1981.  Wastewater Treatment by
         Rooted Aquatic Plants in Sand and Gravel Trenches.  EPA-600/S2-81-091.
         Washington,  DC.

         The objective of this project was to evaluate a patented  process devel-
      oped by the Max Planck Institute (MPI) of West Germany for the treatment
      of industrial wastes, as an energy-efficient method for the  treatment of
      municipal wastewater.  The major goal was to achieve effluents meeting the
      United States Federal Effluent Standards by this novel biological treat-
      ment process that uses a minimal amount of mechanical equipment  and does
      not require a great amount of manpower for normal operation.  An eleven
      month study demonstrated that raw screened wastewater applied to the
      trench system at a rate not exceeding 95 m3/d  (25,000 gpd) could be
      treated to secondary effluent quality.  Spatial requirements were about
      the same as for a septic tank system.


 938. U.S. Environmental Protection Agency.  1981.  Water Quality  and Mangrove
         Ecosystem Dynamics.  Pub. No. EPA-600/S4-81-022.  Washington, DC.

         Field studies were made to determine the relationship between general
      water quality parameters with emphasis on pesticides and metal pollutants,
      and the functioning of the halophytic mangroves. It was concluded, from a
      broad range of ecological sample analysers, that mangroves are relatively
      insensitive to toxic materials in the parts per million range and lower.
      Further, they do not significantly concentrate synthetic organics or
      metals to levels which could be considered harmful to detritus feeders.
1093.  U.S.  Environmental Protection Agency.  1983a.
1094.  U.S.  Environmental Protection Agency.  1983b.
1095.  U.S.  Environmental Protection Agency.  1983c.

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939. U.S. Fish and Wildlife Service.  Refuge Manual  17.  Disease Prevention
        and Control (draft),  pp. 729-757, In;  Populations Management, 7RM17,
        National Wildlife Refuge System.  Department of the Interior, Washing-
        ton , DC.
940. U.S. Forest Service.  1978.  Strategies for Protection and Management  of
        of Floodplain Wetlands and Other Riparian Ecosystems.  GTR-WO-12. Pro-
        ceedings of the Symposium, 11-13 December 1978, Callaway Gardens, GA.
        Department of Agriculture, Washington, DC, 410 p.
941. Vadas, R.L., M. Keser, P.C. Rusanowski, and B.R. Larson.  1976.  The
        Effects of Thermal Loading on the Growth and Ecology of a Northern
        Population of Spartina alterniflora, pp. 54-63.  In G.W. Esch, and R.
        W. McFarlane (eds.), Thermal Ecology II.  Proceedings of a symposium
        held at Augusta, Georgia, 2-5 April 1975.  Technical Information
        Center, Energy Research and Development Administration.  (Available
        from NTIS as CONF -750425).

        Thermal effluents from the Maine Yankee Atomic Power Co. increased the
     plant densities of Spartina alterniflora during the first year of opera-
     tion (1973) but reduced the densities  substantially the second year
     (1974).  Growth and flowering were initiated earlier near the heated
     effluent.  Subsequent growth was reduced in early summer but was more
     normal during the late summer and fall of the first year.  During the
     second year of operation, growth was greatly reduced, and large plants
     never developed.  Biomass and net-production estimates at the control site
     compare favorably with other marshes along the east coast of North
     America.  In the marsh affected by the thermal discharge, biomass de-
     creased by the thermal discharge, biomass decreased by 48% in 1973 and 40%
     in 1974.  The rhizome system of affected plants collapsed in 1974-1975.
     These plants, in contrast to southern  forms, appear to have lost their
     tolerance of high temperatures.  (AA)
942. Valiela, I., M.D. Banus, and J.M. Teal.  1974.  Response of Salt Marsh
        Bivalves to Enrichment with Metal-containing Sewage Sludge and Reten-
        tion of Lead, Zinc, and Cadmium by Marsh Sediments.  Env. Pollution
        7(2):149-157.

        Growth in the bivalves Mercenaria mercenaria and gra_Bsos_trea, virginica
     was not affected by experimental additions of metal-containing sewage
     sludge and fertilizers to salt marsh plots.  Nodiolus demissus, a mussel
     inhabiting the marsh surface, did grow better under the same fertilizer
     treatments.  Input-output, budgets show that Pb was trapped in the sedi-
     ments with virtually no loss to deeper waters.  Zn and Cd also accumulated
     in the sediments but there was some transport away from the salt marsh
     surface.  Increases in Zn and Cd, but not Pb, were detected in the creek
     bottom detritus downstream.  All three species of shellfish showed no
     increases in Pb or Zn contents, but all showed increases in Cd.

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943. Valeila, I., and J. M. Teal.  1972.  Nutrient and Sewage Sludge Enrich-
        ment Experiments in a Salt Marsh Ecosystem.  Intecol. Symp. on
        Physiological Ecology of Plants and Animals in Extreme Environments.
        Dubrovnik, Yugoslovia.
944. Valiela, I., and J. M. Teal.  1978a. Inputs, Outputs, and Interconver-
        sions of Nitrogen in a Salt Marsh Ecosystem.  In R. Jeffereies and A.
        Davy (eds.), Ecological Processes in Coastal Environments.  Brit.
        Ecol. Soc. Symp. Blackwell.
945. Valiela, I., and J.M. Teal.  1978b.  Nutrient Dynamics: Summary and Recom-
        mendations,  pp. 259-263, In; R.E. Good, D.F. Whigham, and R.L. Simpson
        (eds.), Freshwater Wetlands:  Ecological Processes and Management
        Potential.  Academic Press, Inc., New York, NY.
946. Valiela, I., J. M. Teal, and N. Y. Persson.  1976.  Production and
        Dynamics of Experimentally Enriched Salt Marsh Vegetation: Below
        Ground Biomass.  Limnol. Oceanogr. 21:245-252.

        Root growth increased during the early growing season in Spartina
     alterniflora salt marsh plots.  While fertilization with nitrogenous fer-
     tilizer did not affect initial growth, a marked decrease in root biomass
     followed the spring peak particularly where nutrient doses were highest.
     A sharp reduction in roots occurred in enriched areas covered by Spartina
     patens, although as with S_. alterniflora., aboveground biomass increased.
     Roots disappeared during autumn leaving rhizomes as the only part of the
     plants to overwinter.  The maximum standing crop for roots was 0.2 cm
     deep, for rhizomes 2.5 cm.  Net annual underground production was calcu-
     lated from annual increments in dead matter belowground. Total production,
     underground and aboveground, exceeds that of any marine-vegetation ranking
     from 3,900 to 6,600 g m^ yr^ in £3. alterniflora areas and 3,200 to
     6,200 g m^ yr^ in S>. patens areas.  Fertilization increased production
     particularly aboveground where dead plant parts are subject to export.
     (AA)
947. Valiela, I., J. M. Teal, and W. Sass.  1973.  Nutrient Retention in Salt
        Marsh Plots Experimentally Fertilized with Sewage Sludge.  Estuarine
        Coastal Mar. Sci. 1(3):261-269.

        Growing population on the NW Atlantic Coast has increased the contami-
     nation of coastal wetlands with sewage wastes.  Effects of sewage on salt
     marsh ecosystems by experimentally treating salt marsh plots with sewage
     sludge was studied.  Plots 10 m in diameter were fertilized once every two
     weeks with commercially available sewage sludge and nutrient losses mea-
     sured in ebbing tidal water.  The experimental plots were located in
     basins drained by single small ditches to allow sampling of ebbing tidal
     water from each plot.  Concentration of ammonia nitrogen, nitrate+nitrite

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     nitrogen, nitrite nitrogen, phosphate phosphorus and total phosphorus,
     respectively, in water leaving the treated plots was measured.  Sea water
     temperature negatively affected phosphate phosphorus losses.  Principal
     losses were in the form of ammonium and phosphate and were related to dos-
     age applied and height of tide following application.  Salt marsh systems
     are sufficiently flexible to use as much nitrogen as is available regard-
     less of the supply mechanisms. They may act as effective removers of
     excess inorganic nitrogen.  Results suggest that adequately managed salt
     marshes may be considered as potential tertiary treatment systems for sew-
     age effluent.
948. Valiela, I., J. M. Teal, and W. J. Sass.  1975.  Production and Dynamics
        of Salt Marsh Vegetation and the Effects of Experimental Treatment
        with Sewage Sludge.  J. Appl. Ecol. 12(3):973-981.

        The response of salt marsh vegetation to sewage applications is  deter-
     mined.  The seasonal pattern of growth and decay of  organic matter  is
     studied, and changes in the botanical composition of vegetation are
     measured.  Fertilization with a 10-6-4 sewage sludge fertilizer increased
     total peak standing crops of salt marsh vegetation despite the presence
     of considerable amounts of heavy metals and chlorinated hydrocarbons in
     the sludge.  (EL)
949. Valiela, I., J. M. Teal, S. Volkmann, D. Shafer, and E. J. Carpenter.
        1978.  Nutrient and Particulate Fluxes in a Salt Marsh Ecosystem:
        Tidal Exchanges and Inputs by Precipitation and Groundwater.   Limnol.
        and Oceanogr. 23: 798-812.

        Waterborne nutrients enter Great Sippewissett Marsh through  ground-
     water, rain, and tidal flooding. The ebb of tidal water removes  nutrients.
     During summer, uptake by marsh biota leads to net import of  nutrients.  The
     increased export of ammonium in August may be due to leaching from senes-
     cent marsh plants.  There is a net annual export of ammonium, nitrate,
     nitrite, dissolved organic (DON) and particulate (PN) nitrogen,  particu-
     late carbon (PC), and phosphate.  Ammonium, DON, and PN are  the  major
     forms of nitrogen exported.  Nutrient concentrations in coastal  and marsh
     water are correlated, and marsh exports could contribute substantially  to
     nutrient supplies of coastal waters.
        Groundwater entering the marsh provides primarily N03~N and DON. Nutri-
     ent inputs through precipitation consist primarily of DON, NO3~N and NH4~N.
     Particulate materials in rain have a high C:N ratio, contributing little
     to enrichment of the nitrogen-limited salt marsh.  Groundwater  carries
     over 20 times the amount of nutrients brought in by rain.  The nitrogen
     provided by both sources is more than enough to support annual plant
     growth.  Inputs of nitrogen by groundwater are therefore important to the
     nitrogen economy of a salt marsh.
        About half the dissolved inorganic nitrogen brought into  the  marsh by
     groundwater is converted to and exported as PN.  The marsh thus  transforms
     the nitrogen that would have been used by primary producers  into a form
     suitable for consumers such as shellfish.  Large amounts of  apparently
     refractory DON enter the marsh in groundwater and similar amounts are

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     exported by tides. PC exported to coastal water is equivalent to 40% of
     the net annual production of Sjpartina al ternif lor a, the dominant marsh
     plant.  (AA)
950. Valiela, I., and S. Vince.  1976.  Assimilation of Sewage by Wetlands,
        pp. 234-253.  In M. Wiley (ed.), Estuarine Processes, Vol.  1.  Uses,
        Stresses and Adaptation to the Estuary.  New York.  541 pp.

        Wetlands have attracted attention as potential components of waste
     treatment systems because of typically high primary productivity, high
     decomposer activity, anaerobic condition and large adsorptive  areas in the
     sediments.  These properties seem to provide wetlands with the ability to
     degrade and eliminate contaminants in wastewaters, and have been studied
     in a variety of wetland habitats throughout the world (salt and brackish
     marshes, polders, cypress, papyrus and mangrove swamps, freshwater marshes
     and bogs).  Although each wetland type differs, current results from
     marshes show that N and P compounds are removed from contaminated or waste
     waters.  Heavy metals from sludges are retained by marsh muds, but the
     effectiveness varies considerably for different metals.  Petroleum hydro-
     carbons accumulate in marsh muds and may be actively decomposed, particu-
     larly at low levels of contamination.  Chlorinated hydrocarbons have high
     affinities for marsh sediments and can be altered by microrganismal
     activity.  Preliminary evidence indicates that counts of coliform bacteria
     may be reduced in tidal water that floods marshes.  Little is  known about
     the thresholds of wetlands to tolerate the impacts of wastewater, but
     studies in chronically contaminated sites show that marshlands function
     well even in severely polluted areas.  (WE)
951. van der Valk, A. G., C. B. Davis, J. L. Baker, and C. E. Beer.   1979.
        Natural Freshwater Wetlands as Nitrogen and Phosphorus Traps  for Land
        Runoff, pp. 457-467. In P. E. Greeson, J. R. Clark, and J. E. Clark
        (eds.), Wetland Functions and Values: The State of Our Understanding.
        Proceedings of the National Symposium on Wetlands held in Lake Buena
        Vista, FL, 7-10 Nov. 1979.  Tech. Publ. No. TPS 79-2.  Amer.  Water
        Resour. Assoc., Minneapolis, MN.

        All types of natural wetlands behave as N and/or P traps at least
     seasonally and, as a consequence, improve the quality of polluted water
     passing through them.  Annual efficiency of N and P removal by a wetland
     is primarily a function of the wetland's hydrologic regime, litter fall
     pattern, and rate of litter decay.  Improving the efficiency of  N and P
     removal by proper management of a wetland is at least theoretically
     possible in some cases.  (AA)
952. Van Raalte, C.D.  1977.  Nitrogen Fixation in Salt Marshes - A Built-in
        Plant Fertilizer.  Oceanus 20(2):58-63.

        The structure of salt marsh ecosystems and the effect of pollutants on
     them has attracted the interest of agricultural researchers looking for

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     ways to maintain crop yields while  reducing  nitrogen  requirements,   the
     present research, of nitrogen fixation in  such an  ecosystem,  studied a
     salt marsh cord grass, Spartina alterniflora.  The grass  roots  fix  nitro-
     gen at high rates and the bacterial  symbiont in  the root  is  similar to
     that of Digitaria (crab grass).  These studies,  in the  Great Sippewissett
     Marsh on Cape Cod in Massachusetts,  found  that in  plots fertilized  with
     sewage sludge and in plots fertilized only with  nitrogen, marsh grass grew
     taller, looked greener, and was higher in  nitrogen.   There was  no change
     in plots fertilized only with phosphate.   It was presumed that  nitrogen
     fixation must be important in salt marshes.  In  fact  fixation rates were
     very high.  Several years of study  have produced a better understanding of
     nitrogen fixation in salt marshes, with major fixation  sites  identified
     and seasonal rates and environmental factors determining  those  rates
     determined.
953. Van Raalte, C. D., I. Valiela, and J. M. Teal.   1976.  The Effect  of  Fer-
        tilization on the Species Composition of  Salt Marsh Diatoms*  Water
        Research 10(1):1-4.

        The diversity of epibenthic diatoms  in  a  salt marsh is reduced  by
     fertilization with either sewage sludge or urea.  Counts of  500  indivi-
     duals are sufficient to demonstrate  a lower  total number in  the  fer-
     tilized areas as compared with that  of  controls.  Nayicula salinarum
     Grun., which formed 5-9% of the diatoms in the  controls, become  dominant
     in fertilized plots, constituting 20-25% of  the population.   (EL)
954. Vaughn, J. M., and J. H. Ryther.   1974.  Bacteriophage  survival  patterns
        in a Tertiary Sewage Treatment-aquaculture Model System.  Aquaculture
        4(4): 399-407.

        Virus survival is investigated  under  simulated  conditions of  a ter-
     tiary sewage treatment-aquaculture system using a  bacteriophage
     indicator.  Survival time extends  in a pure culture growing algae despite
     environmental conditions usually considered antiviral in nature.   Sur-
     vival is further enhanced by increasing  the concentration of sewage
     effluent in the sewage-sea-water mixtures.  (EL)
955. Veber, K., J. Zahradnik, and I. Breyl.   1980.  Efficiency  and Rate  of
        Elimination of Polychlorinated Biphenyls from Wastewaters by Means  of
        Algae.  Bulletin of Environmental Contamination and Toxicology
        25(6):841-845.

        Polychlorinated biphenyls (PCB) were  eliminated from municipal waste
     water and piggery waste water at efficiencies of up to 100% by algal
     concentration and subsequent lyophilization.  Seenedesmus  acutjis was
     cultivated outdoors in southern Bohemia  for 2-week periods during
     June-September 1978, at average daily temperatures of 19.8 C.  Once a  day
     a volume of the suspension was withdrawn and replaced by the same volume
     of wastewater.  Dilution rates and corresponding elimination efficiencies

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     were as follows: 0.1, 100%; 0.2, 67%; 0.3, 80%; and 0.5, 45%.  Similar
     studies done with Chlamydpmpnas geitleri at an average temperature of
     17.8 C in May and June 1979 showed 0.1, 84%; 0.2, 73%; 0.3, 64%; and 0.5,
     100%.  Although there was a trend toward decreasing efficiency of PCB
     absorption with increasing dilution rate, weather may also have been a
     factor.  The PCB contents of the dry algal biomass at the maximum
     dilution rate were 213 mg per kg for £>. acutus and 157 mg per kg for C.
     geitleri.  (WR)                      ~                               "~
956. Vennes, J. W.  1970.  Microbiology of Sewage Lagoons - Role of Purple
        Sulfur Bacteria in the Stabilization of Industrial Wastes.  Tech.
        Completion Kept. North Dakota Water Resources Research Inst. , Fargo,
        ND.  21 pp.

        Ecologic relationships among various lagoon organisms and chemical and
     physical parameters of the lagoon were attempted.  Pure cultures of the
     purple sulfur bacteria, Chromatium yinosum and Thipcapsa floridana were
     characterized with respect to optimum pH, temperature, and a variety of
     organic substrates.  Experimental and actual lagoon substrate deter-
     minations suggest that the purple sulfur bacteria are predominantly
     responsible for the utilization of volatile fatty acids from the lagoon.
     Furthermore, the majority of the BOD in the lagoon may be attributed to
     these volatile acids.  A variety of microbial and chemical determinations
     were made.  (AA)
957. Vennes, J. W., H. W. Holm, M. W. Wentz, K. L. Hanson, and J. M. Granum.
        1969.  Microbiology of Sewage Lagoons - Effects of Industrial Wastes
        on Lagoon Ecology.  Tech. Completion Rept.  Univ. of North Dakota.
        Grand Forks, ND.

        The report presents some of the ecological changes occurring in  sewage
     lagoons receiving domestic wastes plus potato processing wastes
     (Grafton); cheese manufacturing wastes (Lakota); and domestic wastes
     (Harvey).  Since each lagoon presents unique operating conditions,  the
     results are summarized in topical form.  (AA)
958. Verduin, J.  1969.  Man's Influence on Lake Erie. The Ohio Journal of
        Science.  69(2):65-69.

        Conversion of  northwestern Ohio's great Black Swamp to farm  land  during
     the last half of  the 19th century had a profound, but scantily  documented
     influence on Lake Erie.  Silts, once largely filtered by swampland vegeta-
     tion, were, with  the destruction of that vegetation, carried into Lake
     Erie, reducing light penetration and significantly  altering the lake's
     biota.  More recently enhancement of plant nutrients, especially phosphor-
     us, which has increased 5-fold since 1948, has  supported nuisance levels
     of plant growth creating severe oxygen depletion near the lake  bottom and
     responsible for additional major and undesirable changes in species  compo-
     sition of plant and animal communities.  The solution to this problem is

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      removal  of  plant  nutrients from the waters before they enter Lake Erie.
      The  'Living Filter'  treatment,  in which sewage plant effluents are filter-
      ed through  root zones of  plant  communities, seems most promising.  This
      treatment effectively removes nutrients, converting them to plant pro-
      ducts.   Another model, where effluent from sewage treatment plants is
      passed through a  series of artificial lakes,  processes the effluents
      effectively so that  final-stage lakes are excellent recreational facili-
      ties, and the released waters do not burden aquatic environments.
 959. Verry, E.  S.   1979.   Plant Nutrient Retention in Natural Bogs and Impli-
         cations for Sewage Application.   In J.  C.  Sutherland and R.  H. Kadlec
         (eds.), Wetland Utilization for  Management of Community Wastewater.
         Abstracts of conference held 10-12  July 1979 at Higgins Lake, MI.

         An undisturbed 9.72  ha  watershed in north  central Minnesota having
     two-thirds upland till  soils  with mature  aspen and one-third sphagnum
     (3.24 ha) with mature black spruce  was intensely instrumented to measure
     water-borne nutrient  flow.  We wanted  to  find out how the peatland por-
     tion retained  natural inputs  of N,  P,  K,  Ca,  and Mg resulting from direct
     precipitation  and flows from  the surrounding  upland.  Seasonal  effects
     were measured  during  3  years  (1971-1973)  using three natural periods  for
     input; 1)  spring (mid Nov.  -  mid May), 2)  summer (mid May - mid Sept.),
     and 3) fall  (mid Sept.  - mid  Nov.). Output periods were similar except
     for "fall" when streamflow sometimes extended to mid-Jan.  Natural
     nutrient retention properties can help guide  the timing of sewage efflu-
     ent application.
         The peatland received nutrients  in  direct  proportion to the  amount of
     precipitation  plus upland  flow and  passed  on  nutrients in direct propor-
     tion to the amount of streamflow leaving  it.   In almost all situations
     the peatland retained a portion of  the nutrients that entered it.
     However, retention was  not related  to  water amounts, but rather to un-
     known processes.
         We thought  that spring  rain and  snowmelt might result in greater
     relative flushing rather than retention, but  the opposite occurred.   Gen-
     erally the highest relative retention  and  the highest absolute  retention
     were in the spring.   We also  plotted the absolute nutrient amount retain-
     ed  during each season over the amount  received and found that retention,
     for all nutrients, increased  as  more was received.   Thus, under natural
     ranges, the peatland  was never saturated with nutrients.
         These data  indicate  that effluent application need not be delayed
     until well after snowmelt  because relative retention is highest during
     snowmelt runoff.  This  phenomenon may  be related to luxurious sphagnum
     growth immediately after snowmelt and  chemical adsorption.   Also, natural
     processes exist for retaining increased nutrient loads.   Thus,  effluent
     research should define  the  range of loadings  necessary to reach satura-
     tion so that criteria matching land area or outflow water quality to
     loading are possible.   (AA)
960. Wagner, D.D.  1973.  An Investigation of the Physical Impact  of  Sewage
        Outflow on a River-Estuarine Environment.  Trident Scholar Project
        Rept. No. 50.  U.S. Naval Academy.  Annapolis, MD.

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        The impact of the sewage outflow from the treatment plant in Anna-
     polis, Eastport, Maryland, into the Severn River is explored.  A buoyant
     plume model of the behavior of the sewage upon introduction to the re-
     ceiving water is presented which indicates dilution to a 3% sewage con-
     centration by the time the waste reaches the surface.  Bottom currents
     are investigated and compared with surface flows to assess their effect
     on dispersion of the contaminants.  Finally, a tidal flushing model  is
     constructed that predicts a uniform sewage pollution excess over the
     conditions of the Chesapeake Bay of approximately  .05% throughout the
     Severn River.  The effects of various environmental changes on the system
     are discussed as predicted by modification of the  numerical model.   Under
     present circumstances, no detrimental physical impact on the environment
     can be shown to exist.  (AA)
961. Walker, M., and A. T. Ingham.  1980.  Alternative Systems for Wastewater
        Management.  Compost Science/Land Utilization 21(6):31-33.

        Three promising alternatives to the construction of conventional
     gravity sewers are proposed for rural communities.  These systems are
     intended to reduce the cost of sewage collection in these locations.  The
     design and operation of the vacuum sewer, pressure sewer, and small
     diameter gravity sewer are described.  Options for the treatment and
     disposal of collected sewage include sand filters, lagoons, aquaculture
     marsh systems, overland flow, and other land treatment alternatives.
     Management considerations for these systems are surveyed.   (EL)
962. Walker, W. R., and W. E. Cox.  1974.  Wastewater Irrigation:  Its Legal
        Impact.  Water Spec\.rum 6(2): 15-22.

        EPA is encouraged by the Federal Water Pollution Control Act Amend-
     ments of 1972 to develop revenue-producing waste treatment facilities:
     specifically, recycling potential sewage pollutions in connection with
     agriculture, silviculture, or aquaculture.  The land use, groundwater,
     and surface water rights connected with wastewater irrigation at the
     state and municipal levels are explained.  The ultimate test  of feasi-
     bility for a given wastewater irrigation installation may well depend on
     legal constraints.  (EL)
963. Walsh, G. E., L. H. Bahner, and W. B. Horning.   1980.  Toxicity  of  Tex-
        tile Mill Effluents to Freshwater and Estuarine Algae, Crustaceans,
        and Fishes.  Environmental Pollution; Series  A: Ecological  and Bio-
        logical 21 (3):169-179.

        The toxicity of secondary waste effluents  from textile manufacturing
     plants was determined with freshwater (algae-Selenastrum caprIcprnuturn,
     crustacean-paphnia jgulex, minnow-pimephales promelus)  and estuarine
     (algae-Skeletonema costaturn, shrimp-Palaemonetes pugio, min-
     nows-Cyprinodon variegatus) organisms.  D. Pulex was  generally the  most
     sensitive animal, but no animal responded to  all wastes.  Growth of the

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     algae £!. capricprnutum and S_. cpstatum was  affected  by  all  wastes,
     through either inhibition or stimulation.   Some wastes  were stimulatory
     to §.• costatum at low concentrations  (i.e.,  less  than 1%) but  inhibitory
     at high concentrations (i.e., greater than  50%).  The concentration  of
     waste that stimulated growth of IS. costatum by 20% compared with controls
     (SC20) was calculated and used with EC50 values for  the survival of  D.
     pulex to estimate the potential impact of the wastes in relation to
     volume of discharge.  (PA)
964. Walters, A.M., R.O. Teskey, and T.M. Hinckley.   1980.   Impact  of  Water
        Level Changes on Woody Riparian and Wetland Communities.  Vol  VII:
        Mediterranean Region Western Arid and Semi-Aird Region.   FWS/OBS-78/93.
        U.S. Fish and Wildlife Service.

        This volume covers the important woody plant  species in the Mediterran-
     ean, Western Arid and Semi-arid Regions.  These  regions cover  Arizona, New
     Mexico, California, Utah, Nevada, southern Idaho, eastern Washington  and
     Oregon.  This discussion centers primarily on the lower elevations  of
     these states, while the mountainous areas of the western states will  be
     discussed in a subsequent volume.
        This review attempts to synthesize existing information on  the effect
     of water level changes on woody plants found in  riparian and wetland  com-
     munities.  In addition, the effect of drought on woody  plants  of  these
     regions is described.  Riparian vegetation is normally  exposed to high
     groundwater levels as well as to periods of excess water due to flooding.
     The effect of excess water often causes stress to the plant  resulting  in
     decreased growth or even death.  A plant's response to  flooded conditions
     depends on many factors, including the species tolerance, water level,
     duration of flooding and time of year.  Available information  for individ-
     ual species of the region has been listed in a tabular  format.
        In this arid region of the United Sttes the majority of precipitation
     falls in the dormant season, thus flooding effects are  not as  prominant as
     in other sections of the United States.  Drought and the plant's  resist-
     ance to drought are also important factors to consider.  Therefore, a
     table of species responses to drought for these  regions has  been  included.
965. Wanielista, M P., and W. W. Eckenfelder, Jr.  (eds.)  1978.  Advances  in
        Water and Wastewater Treatment-Biological Nutrient Removal.  Papers
        presented in part at a conference on biological nutrient removal
        alternatives held at Orlando, Florida, March  1978.

        This book presents the state-of-the-art of biological nutrient removal
     alternatives by incorporating the results of  recent  research with design
     and operational details.  The 13 papers discuss  the  fundamentals of
     process selection, N control processes, the kinetics of N removal,
     carousel activated sludge, 3 stage processing in nutrient removal, the
     Bardenpho process, the Phostrip system for removing P from wastewater,
     the use of water hyacinths in wastewater treatment, the use of cypress
     swamps in nutrient removal and wastewater recycling, biological fluidized
     beds, biological nutrient removal sludge processing, wastewater treatment
     cost effect and rotating biological contractors.  Tables, diagrams,  photo-

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     graphs, and a subject index complement the text.  Individual papers  are
     indexed in bioresearch index.   (BA)
966. Water Films.  Affluent Effluent: New Choices in Wastewater Treatment.
        Watertown, MA.  A 40-minute film on wastewater treatment  issues  and
        options.  The film looks at the practical and creative wastewater
        treatment systems of 17 communities across the country.   Water Films
        30 Bates Road, Watertown, MA.
967.  Webster, B.  1975.  Bulrushes Being Used in Artificial Marshes  to  Filter
        Water.  New York Times. March 9, 1975.  p. 47.

        Bulrushes are being used in artificial marshes to screen out  bacteria
     and chemicals that too often pollute much of the world's freshwater
     supply.  The marsh water filtration method and other systems  involving
     the use of aquatic plants and animals are being investigated  as  possible
     alternative, relatively inexpensive, ways of reclaiming wastewater.  More
     than 1,000 such projects are being conducted in the U.S. alone,  mostly by
     small communities, summer camps, and recreation areas.  (EL)
968. Webster, L. F.  1974.  Forging the Missing Link.  Wat. and Pollut. Con-
        trol 112(3):42-45.

        The author discusses the possibility of using a closed system where
     the particular ecology would be self-supporting and -sustaining and
     include man, to treat organic wastes.  Work being done at the Woods Hole
     Oceanographic Institute on a complex, multi-species food chain serving
     the dual function of a tertiary sewage treatment process, removing N and
     P before discharge into the ocean, and an aquaculture system producing a
     primary crop of shellfish, is described and the system flow-charted.  The
     work carried out,  and organisms used, to date are reviewed.  It is cal-
     culated that a population of 50,000 would require 50 ha for algal farms
     and 1 ha for oyster production, compared to 40 ha, required by tradition-
     al land-disposal sewage treatment facilities.  (AL)
969. WED Enterprises.  1976.  Sewage Filtration and Energy Production  in Using
        Water Hyacinths.  EPCOT Tech. Meet.  July 1, 1976.  Walt Disney World,
        Orlando, FL.
970. Welch, E. B., and T. Lindell.  1980.  Ecological Effects of Wastewater.
        Cambridge University Press, New York.  337 pp.
971. Weller, M. W.  1975.  Studies of Cattail in Relation to Management  for
        Marsh Wildlife.  Iowa State Journal of Research 49(4)-.383-412.

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972. Weller, M. W.   1978.  Management  of  Freshwater  Marshes  for Wildlife,
        pp. 267-284.  In R. E. Good, D. F. Whigham,  and R. L.  Simpson (eds.),
        Freshwater Wetlands: Ecological Process  and  Management Potential.
        Academic Press, Inc., New York.

        Although commonly practiced  on wildlife  management areas,  marsh manage-
     ment is poorly  founded in theory  and as a predictive science.   Major
     objectives have been to preserve  marshes in a natural state and to main-
     tain their productivity.  System  or  community-oriented  management  techni-
     ques are encouraged as most likely to meet  diverse public needs, whereas
     species-specific management is  more  difficult,  costly and limited  in
     application.
        The structure of a marsh is  a  product of basin shape,  water regimes,
     cover—water interspersion, and plant species diversity.   Resultant vege-
     tative patterns strongly influence species  composition  and size of bird
     populations.  Food resources influence mammals  as well  as birds.  Species
     richness (i.e.  number of species) may be the most simple  index to  habitat
     quality, although various diversity  indices need  further  evaluation.
        Marshes are  in constant change, and wildlife species have  evolved
     adaptations of wide tolerance or  mobility.   Throughout  the Midwest, water
     levels and muskrats (Ondatra zibethicus) induce most vegetative change,
     and the pattern of vegetation,  muskrat and  avian  responses are predictable
     in a general way.  This short-term successional pattern in marshes forms  a
     usable management strategy.  Various  ramifications are  discussed that may
     enhance or perpetuate the most  beneficial stages.
        Artificial management practices are discouraged as costly  and of short-
     term value whereas systems based  on  natural successional  patterns  produce
     the most ecologically and economically sound results.   Public pressures
     for single-purpose management often  increase as management potential
     increases, but  such problems often can be avoided by advance  planning and
     public relations.
        Marsh management projects for  wildlife have  rarely been adequately
     evaluated because of cost, manpower,  and inadequate experimental study
     areas.  Some high priority, management-oriented research  goals are
     suggested.  (AA)
973. Weller, M. W., and L. H. Fredrickson.   1973.  Avian Ecology of  a Managed
        Glacial Marsh, pp. 269-291.  Iii The  Living Bird, Twelfth Annual,  1973,
        Cornell Laboratory of Ornithology, Ithica, NY.
974. Wellings, F. M.  1975.  Interim Progress Report on Effect  of  Cypress
        Domes on Viruses in Effluent.  In H. T. Odum, K.  C.  Ewel,  J.  W.
        Ordway, and M. K. Johnston  (eds.), Cypress Wetlands  for Water Manage-
        ment, Recycling and Conservation, Second Annual Report. Univ.  of
        Florida, Gainesville.
975. Wellings, F.M. 1976.  Viral Aspects of Wetland Disposal  of  Effluent,  pp.
        297-305.  In;  D.L. Tilton, R.H. Kadlec, and C.J. Richardson  (eds.).
        Proceedings of a National Symposium on Freshwater Wetlands and  Sewage
        Effluent Disposal. Univ. of Michigan, Ann Arbor.

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        From a virological standpoint, the effluent from a cypress dome  treat-
     ment plant serving a small trailer court is relatively comparable with
     that of urban treatment plant effluent.  The quantities of virus entering
     the plants are comparable, but the variety of virus types entering  the
     dome is limited.  Since this dome represented a burned out dome, the  effi-
     cacy of sewage treatment by an undisturbed cypress dome has not been
     tested.
976. Wellings, P.M.  1979.  Technical Difficulties in Determining Virus  Sur-
        vival in Wetlands.  In; J.C. Sutherland and R.H. Kadlec  (eds.),  Wet-
        land Utilization for Management of Community Wastewater.  Abstracts of
        a Conference held 10-12 July 1979.

        All viruses which are replicated in the alimentary tract of man  are
     discharged as an integral part of man's excrement.  Over the past decade
     studies have shown that many of these viruses which enter the treatment
     plant are not inactivated but are discharged as infectious virus in the
     effluent and sludge.  Because the viruses are solids-associated they  are
     protected from many of the physiochemical, potentially virus inactivating
     processes encountered during passage through the treatment plant.
        Although terminal chlorination inactivates some of the surviving
     virions, studies have demonstrated the presence of 100 to 750 plaque
     forming units (PFU) of virus per liter of chlorinated effluents.
     Determining the ultimate fate of these surviving virions in effluents
     discharged into wetlands is beset with technical problems, including  the
     obtaining of a representative sample, elution and concentraion of the
     virus present and, finally, assay of the virus per se.
        Based on the above considerations, there is little doubt that the
     presence of even one or two PFU of virus in any field sample must be  con-
     sidered significant since they would represent perhaps 1% to 10% of the
     total virus present.  (AA)
977. Wellings, F. M., A. L. Lewis, and C. W. Mountain.   1974.  Virus  Survival
        Following Wastewater Spray Irrigation of Sandy Soils, pp.  253-260.   In
        J. F. Malina, Jr. and B. P. Sagik (eds.), Virus  Survival in Water  and
        Wastewater Systems.  Austin Center for Research  in Water Resources.
978. Wellings, F. M. et al.  1975.  Demonstration of Virus  in Groundwater
        after Discharge Onto Soil.  Applied Microbiology 29(6):751-757.

        The survival of virus present in  secondary effluents discharged  into  a
     cypress dome was studied.  Isolations were made from concentrates of water
     drawn from 10-foot (304.80 cm) deep  wells.  Data presented  show  vertical
     and lateral virus movement as well as survival within  the dome for  28 days
     during a period of heavy rains when  no effluent was being applied.   Due  to
     the inefficiency of virus concentration procedures, it is proposed  that
     much of the virus present was probably not demonstrated.  A rapid,  rela-
     tively inexpensive concentration technique for sewage  influents  and
     effluents is discussed.  (AA)

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979. Wenck, N.C.  1981.  Wetlands and Organic Soils for the Control of Urban
        Stormwater, pp. 227-240.  In; B. Richardson (ed.), Selected Proceedings
        of the Midwest Conference on Wetland Values and Management.  Minnesota
        Water Planning Board.  St. Paul.  660 pp.

        This paper consists of two major areas of interest.  The first area is
     a review of a research project performed for the EPA to evaluate and
     assess urban runoff treatment methods using non-structural wetland treat-
     ment techniques.  The second area will be to show examples of the appli-
     cation of the technology gained from the research.  Five projects which
     are either constructed or in the design stage will be addressed.  (AA)
980. Wentz, W. A.  1975.  The Effects of Simulated Sewage Effluents on the
        Growth and Productivity of Peatland Plants.  Ph.D. Diss.  Univ. of
        Michigan, Ann Arbor. 112 pp.

        This study was conducted on a north-central Michigan peatland to  deter-
     mine the effects of sewage effluents on the growth and productivity  of
     several common peatland plants.  Emphasis throughout these  experiments was
     on practical application and the consequences of using the  peatland  as a
     disposal site.
        Plants were subjected to varying levels of simulated sewage effluents
     in field and greenhouse experiments during both 1973 and  1974.  Throughout
     the experiments information was collected on changes in aboveground  and
     belowground standing crops, relative growth rates, net production rates,
     changes in nitrogen and phosphorus concentrations, specific leaf areas,
     leaf area index, and related parameters.
        Although some changes in plant species composition and productivity
     are possible, it is unlikely that rapid or extensive degradation of  the
     peatland will occur.  It is possible that denitrification and phosphate
     adsorption onto the peat will have significant effects on the availability
     of plant nutrients.   Assuming that sewage effluent will  add nutrients at
     a rate approximately the same as that tested, it appears  that the efflu-
     ents will have no direct detrimental effects on most of the plants now
     present on the site.
981. Wentz, W. A., R. L. Smith, and J. A. Kadlec.   1974.  A  Selected Annotated
        Bibliography on Aquatic and Marsh Plants and Their Management.  Publ.
        No. 2. Wetland Ecosystem Research Group, Univ. of Michigan, Ann Arbor.
982. Werblan, D., A. van der Valk, C. B. Davis, and R. J. Smith.   1979.
        Artificial Marshes as a Means of Treating Wastes From a Confined Swine
        Feeding Unit.  In; J.C. Sutherland and R.H. Kadlec  (eds.), Wetlands
        Utilization for Management of Community Wastewater.  Abstracts of  a
        conference held 10-12 July,  1979.  University of Michigan-Ann Arbor.

        Eighteen "artificial marshes" (1.2 x 2.4 m plywood  boxes  filled with
     gravel) were constructed and planted with either cattail, burr  reed,  or
     common reed and loaded with one of 3 rates of liquid and solid  swine

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     manure.  One objective was to examine plant viability under these abnormal
     nutrient loads; another objective was to evaluate these marshes as a
     manure renovation system in contrast to an anaerobic lagoon.
        In treatments using solid manure, surface filtration alone removed ap-
     proximately 600 g/m2 wk of volatile solids, 200 g/m2 wk of COD, 6 g/m2 wk of
     and 2 g/m2 wk of P.  In all treatments, 50 g/m2 wk of volatile solids and
     N, 100 g/m2 wk of COD were removed by anaerobic filtration.  Also, 3 g/m2 wk
     of P was precipitated, adsorved onto gravel particles and incorporated by
     microorganisms; organic nitrogen was readily converted to NH4 and 6 g/m2 wk
     of N was removed by volatilization, gravel adsorption and microorganisms.
     Plant uptake of minerals and denitrification did not constitute major
     removal pathways.
        Although the marshes were operated only from May to November, results
     indicate artificial marshes could be a desirable alternative to an
     anaerobic lagoon.  The removals of COD (80-90%), volatile solids (50-90%),
     N (60%), and P (50%) in the artificial marshes were equivalent to removals
     in an anaerobic lagoon.  Offensive odors were not produced and the lush
     vegetation (particularly that of cattail) attracted birds, amphibians, and
     small mammals.  Insect problems were virtually eliminated by maintaining
     the water level at or below the gravel surface. Design modifications which
     might allow winter operations will be discussed.  (AA)
983. Wert, F.S. and V.B. Henderson.  1978.  Feed Fish Effluent and Reel in the
        Savings.  Aguaculture Can be a Cost-Effective Wastewater Treatment
        Alternative.  Water and Wastes Engineering 15(6):38.
984.  Whaling, P.J., R.T. Barber, and J.C. Paul.  1977.  The Distribution  of
        Toxic Metals in Marine Ecosystems as a Result of Sewage Disposal and
        Natural Processes.  North Carolina Water Resources Research Institute,
        Raleigh, NC.  UNC-WRRI Rpt. No. 123. NTIS No. PB-272 644. March. 132 p.

        Industrial discharge of wastes containing high concentrations of
     mercury and other toxic metals has largely been abated by strong federal
     and state regulation.  The recovery of the polluted ecological systems is
     taking longer.  Sewage treatment is a necessary part of our way of life
     and in addition, the natural accumulation of threatening levels of mercury
     and cadmium in unpolluted marine food chains is an aspect of environmental
     concern that must be understood to protect human health.  The role of sew-
     age disposal and natural food chain processes in the distribution of  toxic
     metals in marine ecosystems was examined in several small eastern North
     Carolina estuaries receiving different inputs.  On the basis of metal
     distribution, estuaries receiving treated sewage effluent were clearly
     distinguishable from those not receiving this discharge.  The magnitude
     and spatial extent of the elevated toxic metal distributions was very
     limited in both sediments and organisms.  While some organisms did not
     have ele- vated metal concentrations, the economically important oysters
     did not, nor did the marsh grass, Spartina alterniflora.  The large and
     consistent variation in the amount of mercury and other trace metals
     discharged to the surrounding estuaries by various eastern North Carolina
     towns was traced to variations between the plants. It was not possible to
     account for the origin of the metal variations in wastewaters of these
     small towns which have no known commercial activities using these metals.

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985. Wharton, C.H.  1970.  The Southern River Swamp - A Multiple-Use Environ-
        ment, Bureau of Business and Economic Research, Georgia State Univer-
        sity, pp. 23-43.

        The river-marsh ecosystem was studied to determine potential water
     cleansing abilities.  Water quality data indicate that the Flint and
     Alcovy Rivers and their adjacent swamps apparently have the  ability to
     filter pollutants from water.  Swamp streams appeared to eliminate human
     waste and may have removed toxic pesticides.  Swamps induced deposits of
     silt which may become useful to the biotic community.  The swamp and its
     stream channel seemed intimately associated functionally, and appeared  to
     form a natural hydrogeobiological water treatment system.  The value of the
     cleansing action of 10 km of river and 251 ha of swamp was equivalent to
     sewage treatment for a city of 50,000.  Potentially, the Alcovy River has
     three times this ability, worth $990,000/year.  (AA)
986. Wharton, C. H., and H. P. Hopkins, Jr.   1980.  In-Situ Evaluation  of  the
        Filtering Function of a Piedmont Creek Swamp.  Rept. No.  2-80.   Envir-
        onmental Resources Center, Georgia Institute of Technology,   Atlanta.
        46 pp.  (Available as NTIS Publ. No.  PB 81-142234).

        A small swamp in the northern piedmont region of  Georgia  was  monitored
     from September 1976 to September 1978 to determine if swamps act as sinks
     for nutrients and trace metals.  The total amounts of phosphorous,  ni-
     trates , ammonia, and several trace metals flowing into and out of  the
     Cornish Creek Swamp were monitored during the two year period.   This
     swamp was formed when a portion of the  channelized Cornish Creek was
     filled up with sand and debris, and since the entrance and exit  for this
     swamp is well-defined, we were able to  establish the total amount  of
     water moving through the swamp.  This information, combined  with the
     chemical analysis, allowed us to calculate the total amount  of chemical
     nutrients and metals going into and out  of the swamp over the two  year
     period.  The levels of phosphorous, nitrogen, and metals in  the  waters  of
     the swamp indicate that it is pristine.  Phosphorous was monitored in the
     form of orthophosphate and hydrolyzable  phosphate; nitrogen  was  monitored
     as the ammonia and nitrate species.  The metals: iron, magnesium,  man-
     ganese, aluminum, sodium, and zinc were  also monitored in the water.  A
     small reduction in the total phosphate was observed  after the water
     flowed through the swamp, but more nitrate left the  swamp than entered  it
     during the majority of the months in the study.  Large quantities  of
     ammonia were observed to leave the swamp during the  spring months, but
     the swamp retained a small fraction of  the amounts entering  the  swamp at
     other times.  An abnormally high level  of zinc reached the swamp during
     the period of study from an unknown source, but the  swamp was effective
     in reducing the amount of zinc in the stream during  this period.  When
     phosphates and nitrates were added upstream from the swamp during the
     summer of 1978, the level of these in the water flowing through  the swamp
     increased greatly.  During the two day period of these experiments
     appreciable quantities of the nutrients  remained in  the swamp.   (AA)

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 987.  Wharton,  C.  H.,  H.  T.  Odum,  K.  Ewel,  M.  Duever,  and A.  Lugo.  1977.
         Forested  Wetlands  of Florida,  Their Management and Use.   Kept,  to the
         Division  of State  Planning,  Tallahassee,  FL.   Center for Wetlands,
         Univ.  of  Florida,  Gainesville.   348 pp.


 988.  Whelan,  T.,  J.  T.  Ishmael,  and W.  S.  Bishop.  1976.  Long-term Chemical
         Effects of Petroleum in  South Louisiana  Wetlands, Organic Carbon in
         Sediments and Waters. Marine Pollution  Bulletin 7(8):


 989.  Whigham,  D.  F.   1979.   Effects of Sewage Spray Irrigation on a Freshwater
         Wetland.   I.  Primary Production and Nutrient Standing Stocks.
         Presented at the Annual  Meeting of the American Institute of Biological
         Sciences, held  12-17 August.  Stillwater, OK.
1096.  Whigham,  D.F.   In press.


 990.  Whigham,  D. F., and S. Bayley.  1978.  Nutrient Dynamics in Freshwater
         Wetlands,  pp. 468-478.   In P.  E. Greeson, J. R. Clark, and J. E. Clark
         (eds.),  Wetland Functions and Values: The State of Our Understanding,
         Proceedings  of the National Symposium on Wetlands, Lake Buena Vista,
         Florida.  Tech. Publ.  TPS 79-2.  Amer. Water Resour. Assoc., Minne-
         apolis,  MN.

         The purpose  was to determine if definable trends appear when com-
      parisons  are made between the nutrient absorption capacities of various
      types of  fresh  water wetlands.  The review showed that there are few data
      available for comparison,  with the exception of N and P in above ground
      vegetation.  Data from mass balance studies are almost nonexistent, which
      makes it  impossible to determine the conditions necessary for specific
      types of  wetlands to annually lose, gain, or be in balance with regard to
      nutrient  fluxes.  The only discernable trend was that wetlands with
      predominantly organic substrates accumulated less N and P in the above
      ground vegetation, yet the peat substrates seem to be capable of
      long-term storage of N and P.  Recommendations for future research on
      nutrient  dynamics in fresh water wetlands are discussed.  (AA)


 991.  Whigham,  D. F., J. McCormick, R. E. Good, and R. L. Simpson.  1978.
         Biomass and Primary Production  in Freshwater Tidal Wetlands  of the
         Middle Atlantic Coast, pp. 3-20.  In R. E. Good, D. F. Whigham, and R.
         Simpson (eds.), Freshwater Wetlands: Ecological Processes and Manage-
         ment Potential. Academic Press, Inc., New York.

         Although there are many measurements of peak standing crop and  several
      estimates of aboveground annual net production, there are few accurate
      estimates of total net primary production for Middle Atlantic coastal
      freshwater tidal wetlands.  Estimates  of biomass  and production vary
      widely and between vegetation types.   The variability appears to be due to
      samplilng techniques  and/or the heterogeneous  nature of  freshwater tidal
      wetland vegetation.  Undoubtedly,  all  estimates of primary production are

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     low because they do not include data on growing season mortality of plants
     and plant parts, herbivore consumption, and belowground production.  Most
     estimates of primary production are also low because freshwater tidal wet-
     land communities undergo a series of physiognomic changes during the grow-
     ing season and the measurements of primary production do not account for
     those seasonal patterns.  Comparatively, freshwater macrophyte production
     in tidal wetlands appears to be at least equal to that of brackish water
     wetlands at the same geographic latitude.  (AA)
992. Whigham, D. F., and R. L. Simpson.  1975.  Ecological Studies of the
        Hamilton Marshes Progress Report for the Period June 1974 - January
        1975.  Rider College, Lawrenceville, NJ.
993. Whigham, D. F., and R. L. Simpson.  1976a. Sewage Spray Irrigation in a
        Delaware River Freshwater Tidal Marsh, pp. 119-144.  In D. L. Tilton,
        R. H. Kadlec, and C. J. Richardson (eds.), Proceedings of a National
        Symposium on Freshwater Wetlands and Sewage Effluent Disposal.  Univ.
        of Michigan, Ann Arbor.

        High application rates of chlorinated effluent to a freshwater tidal
     marsh in New Jersey, caused changes in the vascular plant species com-
     position of the wetland.  Perennials have not been affected by the
     chlorine and clones at several species (sweet flag, cattial, rice, cut
     grass, arrow arum) have increased in aerial  extent.  Some annuals will
     decline in importance  (touch-me-not, bur marigold) while others will
     increase (hallbeard tearthumb).  Annual species composition can be regu-
     lated by controlling the spray regimes.  Vascular plants acted as nutri-
     ent sinks during the summer and nutrients were released during the fall
     and winter with most of the nutrients being  released within a month of
     the end of the growing season.  There was also a noticeable increase in
     the amount and coverage of epibenthic algae.
994. Whigham, D. F. , and R. L. Simpson.  1976b. The Potential Use of Fresh-
        water Tidal Marshes in the Management of Water Quality in the Delaware
        River, pp. 173-186.  In J. Tourbier and R. W. Pierson, Jr.  (eds.).
        Biological Control of Water Pollution.  Univ. of Pennsylvania Press,
        Philadelphia.

        A summary of the studies concerning functional aspects of a freshwater
     tidal marsh and its potential use in water quality management.  Research
     includes vegetation composition, primary production, soil algal distribu-
     tion and biomass, soil nutrient and organic matter content, plant nutri-
     ent content, nutrient movement through marsh by surface water  analysis,
     and movement of detritus in and out of marshes.  The authors conclude
     that "high marsh areas may be acting as a nutrient sink during summer
     months and that perhaps the pond-like areas of the marsh may be playing a
     similar role in the winter...", "...it would appear that freshwater tidal
     marshes may also be capable of performing tertiary treatment."  (WE)

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995. Whigham, D. F., and R. L. Simpson.  1977.  Growth, Mortality, and Biomass
        Partitioning in Freshwater Tidal Wetland Populations of Wild Rice
        (Zizania aquatica var. aguatica).  Bull. Torrey Bot. Club.  104:
        347-351.
996. Whigham, D. F., and R. L. Simpson.  1978.  Nitrogen and Phosphorus Move-
        ment in a Freshwater Tidal Wetland Receiving Sewage Effluent, pp.
        2189-2203.  In Coastal Zone '78, the Proceedings of the Symposium on
        Technical, Environmental, Socioeconomic, and Regulatory Aspects of
        Coastal Zone Management.  Am. Soc. Civil Engineers, New York.

        Recent work on nutrients in tidal wetlands has led to the suggestion
     that they can be managed for treatment of sewage effluent.  In this paper
     we report some results of a three year study on the effects of spraying
     chlorinated secondarily treated sewage effluent on a Delaware River
     freshwater tidal wetland near Trenton, New Jersey.  Biomass and standing
     crops of N and P at the end of 1975 and 1976 growing seasons did not
     differ between experimental areas and control sites.  The only consistent
     response of the aboveground vegetation was that plant material in areas
     receiving effluent had significantly greater %N and %P concentrations.
     Total N, %N, total P and %P of plant litter was significantly greater in
     the experimental areas.  There were no significant substrate responses
     due to spray irrigation.  Tide cycle studies showed that the irrigated
     wetland was a sink for N and an exporter of P.  (AA)
997. Whigham, D. F., and R. L. Simpson*  1979.  Effects of Sewage Spray Ir-
        rigation in a Freshwater Tidal Wetland.  I.  Primary production and
        Nutrient Standing Stocks.  Presented at Annual Meeting Ecol. Soc. of
        America.
998. Whigham, D. F., R. L. Simpson, and K. Lee.  1980.  The Effect of Sewage
        Effluent on the Structure and Function of a Freshwater Tidal Marsh
        Ecosystem.  Tech. Completion Report to Office of Water Resources and
        Technology.  Rider College, Laurenceville, NJ.  160 pp.

        The effects of spraying chlorinated secondarily treated sewage on a
     Delaware River freshwater tidal wetland for three years was studied.
     Macrophyte net primary production was significantly lower in the ex-
     perimental sites receiving sewage than in the no treatment controls in
     1975 but not in 1976 or 1977.  Diversity of annuals was reduced in the
     experimental sites largely due to the elimination of annuals.  Although
     percent N and P were generally high in the vegetation of experimental
     sites, there was little difference in total N and total P between treat-
     ments and controls.  Macrophyte decomposition rates were little affected
     by sewage application.  Substrate N and P were not significantly dif-
     ferent between sites, but surface litter of the experimental sites ac-
     cumulated N and P.  Epibenthic algae may contribute to this accumulation.
     Water quality studies showed the high marsh to be metabolically active.
     Tide cycle flux studies indicated that up to 40% of the N added to the

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      wetland has assimilated  during  the  late  spring-early summer period.
      Conversely, there was a  net  loss  of P from  the  wetland.   These results
      are compared with those  of similar  studies  in other wetlands.   It is
      concluded that the strongly  pulsed  tidal  regimes  of the  wetland,  the low
      organic content substrate, and  the  eutrophic  nature of the Delaware  River
      waters contribute to the inability  of the wetland to efficiently
      assimilate nutrients from sewage.   (AA)
1097. wile, I. et al.  In press.
 999. White, D. H., and M. T. Finley.   1978.  Uptake  and Retention of Dietary
         Cadmium in Mallard Ducks.  Environmental  Research 17:53-59.

         Adult mallard ducks fed 0, 2,  20,  or 200  ppm of cadmium chloride in the
      diet were sacrificed at 30-day intervals  and tissues were  analyzed for
      cadmium.  No birds died during the  study  and body weights  did not change.
      The liver and kidneys accumulated the highest levels of  cadmium.   Tissue
      residues were significantly  correlated in all treatment  groups and
      residues increased with treatment level.   Hematocrits and  hemoglobin
      concentrations were normal in all groups  throughout the  study.   Little
      cadmium accumulated in eggs  of laying hens,  but egg production was sup-
      pressed in the group fed 200 ppm.
1000. White, D. H., M. T. Finley, and J. F. Ferrell.   1978.   Histopathologic
         Effects of Dietary Cadmium on Kidneys  and Testes  of  Mallard Ducks.
         Journal of Toxicology and Environmental Health  4:551-558.

         Mallard ducks fed 2, 20, or 200 ppm  cadmium  chloride were  sacrificed at
      30, 60, and 90 d. No mortality occurred during  the study and  body weights
      remained unchanged.  Kidney weights of  the 200-ppm group were sigfnifi-
      cantly greater after 60 and 90 d than those of  controls; also,  testis
      weights were significantly lower after  90 d.  Kidneys of ducks fed 2 and
      20 ppm cadmium were relatively unaffected; however,  slight  to severe kid-
      ney lesions were found in the 200-ppm group after  60 d  of treatment.   No
      significant lesions were found in mallard testes after  feeding 2 ppm cad-
      mium in the diet, and only a few birds  in the 20-ppm group  showed slight
      to moderate gonad alterations.  After 90 d of treatment,  however,  testes
      of males fed 200 ppm had atrophied and  the spermatogenic process had
      ceased.  This study should provide important information for  the inter-
      pretation of cadmium levels found in kidneys and testes of  wild ducks.
      (AA)
1001.  Whitton, B.A.  1971.  Toxicity of Heavy Metals to Freshwater  Algae:   A
         Review,  Phykos, 9, 1971, pp. 116-125.

         This text provides a literature review on toxicity  of heavy  metals to
      freshwater algae.  Emphasis was put on effects of exposure  to heavy  metals
      over long periods and adaptation potential of algae to heavy  metals.

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1002. Wikum, D., and M.  Ondrus.  1980.  The Drvunmond Bog Project-Growth of
         Selected Plant  Species as a Function of Foliar and Peat Substrate
         Nutrient Concentrations.  Report to the U.S. Forest Service. Univ. of
         Wisconsin,  Stout. 38 pp.

         The following results were reported on this wastewater treatment site
      in Wisconsin:  1) measurable increases in nutrient content of sediment
      over a one year period; 2) reduction in levels of ash and calcium in
      black spruce,  labrador tea, and leatherleaf foliage; 3) no measurable
      damage to plant community during the study period; 4) measurable changes
      in peat and foliar levels of phosphorus, calcium, and ash content.  The
      authors suggested  that these parameters be measured during future re-
      search at the  site.  (EP)
1003. Wilcox, W.  H.  1979.  The Effect of Nitrogen and Phosphorus Enrichment on
         Salt Marsh Community Structure.  Ph.D. Oiss.  Univ. of Tennessee.
         Nashville.
1004. Wile, I.  1980.  An Experimental Approach to Wastewater Treatment Using
         Natural and Artificial Wetlands.  Progress report, 27 October 1980.
         Ontario Ministry of the Environment, Ontario, Canada. 4 pp. plus
         attachment.

         A natural marsh site located near the town of Bradford, Ontario, and a
      multicelled artificial marsh system near the town of Listowel, Ontario,
      are being assessed for their potential for year-round sewage treatment.
      The diked natural marsh has been monitored for background nutrient
      budgets for the last two years.  The addition of partially treated wastes
      from the sewage treatment plant were scheduled to commence in 1981.  The
      artificial marsh operations began in August 1980.  The preliminary
      results obtained during the first 2 months of the latter operation and
      information on the design of the facility are included in this report.
      (EP)
1005. Wile, I., G. Palmateer, and G. Miller.  1981.  Use of Artificial Wetlands
         for Wastewater Treatment, pp. 255-272.  In; B. Richardson (ed.),
         Selected Proceedings of the Midwest Conference on Wetland Values and
         Management.  Minnesota Water Planning Board.  St. Paul.  660 pp.

         In Ontario, sewage lagoons are a common method for wastewater treatment
      in rural communtiies or small urban centers.  However, receiving waters
      are frequently limited in their capacity to provide adequate dilution for
      the effluents, leading to proliferation of aquatic plants and fluctuating
      oxygen levels.  The alternative approach of storing the wastes during
      periods of low flow is land consumptive, and mechanical systems are gener-
      ally beyond the financial capabilities of small municipalities.
         Since wetlands may provide a viable treatment alternative, an experi-
      mental study using an artificially constructed wetland was initiated in
      1979.  This study will attempt to define the degree of pretreatment

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      required prior to waste discharge  to the wetlands, loading  rates,
      retention times and water depths for optimal operation of these types of
      systems.
         The experimental facility consists of five separate systems and  a small
      control marsh for monitoring rates of evapotranspiration.   Construction of
      the marshes was completed in July  1980.  Following planting with cattails,
      Typha spp., sewage additions were  started in August  1980.   Good nutrient
      removal rates were observed during the ice-free period, with total  phos-
      phorus concentrations of <0.2 mg 1~^ in the marsh effluents.  Similar-
      ly, BOD and suspended solid loadings were reduced by more than 95%  during
      passage through both marshes receiving raw aerated sewage or partially
      treated wastes from the existent east lagoon.  During the winter period,
      the efficiency of the marsh sewage tretment systems  declined substantial-
      ly.  Prior to the onset of winter, loadings of sewage influents to  the
      marsh systems were more than doubled to avoid freezing in the pipes and
      outflow chambers.  Concurrent with the increased flows, volumes within the
      marsh cells were reduced by ice formation, with resultant changes in
      retention times from approximately 8 days in the summer months to less
      than 3 days.  These factors contributed to the reduced performance  of the
      experimental marshes during the winter months.  (AA)
1006. Williams, B., and M. B. Murdoch.  1969.  The Potential Importance of
         Spartina alterniflora in Conveying Zinc, Manganese, and Iron into
         Estuarine Food Chains, pp. 431-439.  In D. J. Nelson and J. C. Evans
         (eds.), Proc. 2nd Nat. Symp. Radioecology.  NBS.  Springfield, VA.
1007. Williams, T. C.  1976.  The Feasibility, Planning, and Construction of
         Peatland Tertiary Treatment Systems, pp. 331-343.  In D. L. Tilton, R.
         H. Kadlec, and C. J. Richardson (eds.). Proceedings of a National
         Symposium Freshwater Wetlands and Sewage Effluent Disposal.  Univ. of
         Michigan, Ann Arbor.

         The "feasibility" of adopting wetlands irrigation as an integral part
      of tertiary wastewater treatment involves finding satisfactory answers to
      the concerns of the local project Authority, the service area population,
      regulatory agencies, and scientific investigators.  The concerns are for
      economy, effectiveness, environmental compatabi]ity, and aesthetics.
      Communication and publicity efforts have helped forge feelings of common
      interest and working together among the separate township governments in
      the Houghton Lake Area sewer Authority.  The Houghton Lake Wetlands Study
      is a landmark in tertiary treatment, and in the future may rival upland
      irrigation in feasibility for rural and resort communities.  (AA)
1008.  Williams, T.  C.   1979.  The History and Development of the Houghton Lake
         Wetland Wastewater Treatment Project.  In J. C. Sutherland, and R. H.
         Kadlec (eds.), Wetland Utilization for Management of Community Waste-
         water.  Abstracts of a conference held 10-12 July 1979 at Higgins
         Lake, MI.

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         The Tri-Township wastewater treatment system serving the Houghton Lake,
      Michigan recreation area population of 15,000,  consists of  aeration-stab-
      ilization and storage ponds,  and tertiary treatment provided by a natural
      peat  wetland of 600 acres size.  Completion of  the wetland  treatment
      facilities  in early Summer 1978 was the final step in a 15-year effort.
      The wetland alternative for tertiary treatment  represents savings in land
      and construction costs, relative to upland irrigation, of approximately
      $1,000,000.  (AA)
1009.  Williams, T.  C.   1980.   Wetland Irrigation Aids Nature and Man.  Water
         and Wastes Engineering 17(11):28-31.

         Two wetlands  in Michigan (1 natural and 1 artificial) provide inexpen-
      sive tertiary treatment and benefit from the nutrients.  The facility at
      Houghton Lake, Michigan, discharges to a natural 500 acre peat marsh.
      The wetlands  irrigation system consists  of gated, 12 in Al irrigation
      pipe, supported by a wooden, elevated walkway suspended on 2 in piling.
      The walk permits easy access for operation and maintenance.  The system
      has been in full-scale operation for 2 irrigation seasons, each 4-6 mo
      long.  During the summers of 1978 and 1979, an average of 85 million gal
      of effluent were applied to the 500 acre marsh.  The wastewater contains
      4 mg/1 P, 0.6 mg/1 NH3-N, and 5.4 mg/1 NO3-N.  A high level of treatment
      is achieved with minimal impact on the marsh.  The system at Vermont-
      ville, Michigan, has developed an artificial wetland.  Treatment
      facilities were completed in 1972 and have a capacity of 0.30 mgd.  The
      system consists of 2 facultative ponds and 4 food irrigation fields
      located on the hill next to a small tributary to the Thornapple River.
      The Vermontville system presents excellent opportunities for the pre-
      servation of plant and wildlife.  (PA)
1010. Williams, T. C., and J. C. Sutherland.  1979.  Engineering, energy, and
         Effectiveness Features of Michigan Wetland Tertiary Wastewater Treat-
         ment Systems, pp. 141-173. In R. K. Bastian, and S. C. Reed  (eds.),
         Aguaculture Systems for Wastewater Treatment: Seminar Proceedings and
         Engineering Assessment.  EPA 430/9-80-006.  U.S. Environmental Pro-
         tection Agency, Office of Water Program Operations, Municipal Con-
         struction Division. Washington, DC.

         The results of two studies done on different wetlands in Michigan that
      receive secondary treated, non-chlorinated wastewater are summarized in
      this report.  Both operations, one located at Vermontville and  the other
      at Houghton Lake, have resulted in the effective removal of 98% to 100%
      of phosphorus through contact with reactive  soils.  The operation and
      maintenance, design, and capital and land costs are compared for these
      systems.  The Vermontville site (11.5 acres) has flood irrigation fields
      that have overgrown with volunteer wetland vegetation (mainly cattails).
      The Houghton Lake site is a natural peat wetland approximately  600 acres
      in size.  (EP)

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1011. Williams, T. C., and J. C. Sutherland.  Technical Aspects of the
         Tri-township (Houghton Lake, Michigan) Peat Wetland Tertiary Treatment
         System.  Presented at the Water Pollution Control Federation Confer-
         ence (Session 44), 11 October 1979, Houston, TX.  15 pp.
1012. Williams and Works, Inc.  1978.  Operation and Maintenance Manual.
         Houghton Lake Sewer Authority, Houghton Lake, MI.
1013. William and Works, Inc.  1979.  Reuse of Municipal Wastewater by Volun-
         teer Freshwater Wetlands.  Interim Report.  William and Works, Inc.
         Grand Rapids, MI. 121 pp.
1014. Windom, H.L. 1977.  Ability of Salt Marshes to Remove Nutrients and Heavy
         Metals from Dredged Material Disposal Area Effluents.  Army Engineer
         Waterways Experiment Station, Vicksburg, Mississippi.  Tech. Rpt.
         D-77-37.  December.  100 p.

         Experimental raceways were constructed in a salt marsh adjacent to a
      dredged material confinement area to evaluate use of this environment as
      an overland flow advanced treatment system for effluent resultilng from
      dredged material disposal.  The program was designed to determine the
      ability of salt marsh systems to remove nitrogen, phosphorus, iron, man-
      ganese, cadmium, copper, nickel and zinc from effluent.  Application rates
      varied between 0.06 and 3.7 acre inches per day and concentrations of the
      contaminants were determined in the effluent from the disposal area and
      dishcarge from the explerimental system.  All contaminants were found to
      decrease in the effluent as it passed through the experimental raceways.
      Removal efficiencies for nitrogen and phosphorus varied up to 50 and 70
      percent, respectively.  Mean metal removal efficiencies ranged between 15
      and 32 percent.  Efficiency of removal appeared unrelated to application
      rate but did appear to increase with concentration.  Contaminants accumu-
      lated to sediments with only minor increases in salt marsh vegetation.
      Although results indicate nutrients and heavy metal concentrations in
      effluents from dredged material disposal areas can be reduced during pass-
      age through a salt marsh, conclusions about efficiency or removal may be
      influenced by the size of the experimental system used.
1015.  Witter, J.  A.,  and S.  Croson.  1976.  Insects and Wetlands, pp. 271-295.
         In D. L. Tilton, R. H. Kadlec, and C. J. Richardson (eds.), Pro-
         ceedings of  a National Symposium on Freshwater Wetlands and Sewage
         Effluent Disposal.   Univ. of Michigan, Ann Arbor.

         An exploratory study of insect fauna in Houghton Lake Marsh, Michigan,
      conducted in 1973 is summarized in this report.  Other contents include a
      short literature review of other faunistic studies in wetlands, a review
      of the objectives of various insect sampling programs, a comparison of
      relative and absolute  sampling techniques, and a discussion of criteria

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      to be used when selecting insects as indicator species.  Critical areas
      and principles in insect-wetland interactions are addressed.  It is
      recommended that further long-term studies be conducted.  (EP)
1016.  Wolverton,  B.  C.   1975a.  Aquatic Plants for Removal of Mevinphos from the
         Aquatic  Environment.  NTIS Report N75-16206.

         Fragrant waterlily, joint-grass, and rush were used to assess the
      effectiveness  of  vascular aquatic plants in removing the insecticide
      Mevinphos from contaminated waters.  The emersed aquatic plants waterlily
      and joint-grass succeeded in removing 87 and 93 ppm of mevinphos from
      water test  systems in less than two weeks without apparent damage to the
      plants.   Rush, a  submerged plant, removed less—it still contained toxic
      levels of this insecticide after 35 days.  (DM)
1017. Wolverton, B. C.   1975b. Water Hyacinths for Removal of Cadmium and
         Nickel from Polluted Waters.  National Aeronautics and Space Adminis-
         tration.  Tech. Memorandum X-72721.  National Space Tech. Laboratory,
         Bay St. Louis, MS.
1018. Wolverton, B. C.   1979.  Engineering Design Data for Small Vascular
         Aquatic Plant  Wastewater Treatment Systems, pp. 179-192.  In R. K.
         Bastian, and S. C.  Reed (eds.), Aquaeulture Systems for Wastewater
         Treatment: Seminar Proceedings and Engineering Assessment.  EPA
         430/9-80-006.   U. S. Environmental Protection Agency, Office of Water
         Program Operations, Municipal Construction Division.  Washington, DC.
1019.  Wolverton, B.  C.   1981.  Hybrid Wastewater Treatment System Using Anaero-
         bic microorganisms and reed (Phragmites communis).  National Aeronau-
         tics and Space Administration.  Tech. Memorandum TM-X-72739.

         A small hybrid wastewater treatment system consisting of a settling
      tank in series with an anaerobic filter-reed (Phragmites communis)
      treatment cell was evaluated and compared with a similar plant-free
      system.  Data  demonstrated that by combining anaerobic filters, also
      referred to as attached film filters, and vascular aquatic plants a
      synergistic effect is produced which increases the treatment efficiency
      of each individual system.  The plant-free system reduced the 8005 from
      114 to 31 mg/1 in 6 hours as compared to a reduction of 110 to 9 mg/1 in
      the anaerobic  filter-reed system in the same length of time.  The 8005
      and TSS after  24  hours in each component of the plant-free system was
      reduced from 114  to 14 -tig/1 and 51 to 15 mg/1, respectively.  Under the
      same conditions,  the hybrid system reduced the 8005 from 110 to 3 mg/1
      and the TSS from 68 to 6 mg/1.  The hybrid system also reduced the total
      kjeldahl. nitrogen (TKN) from 16.1 to 2.9 mg/1, total phosphorous (TP)
      from 4.4 to 2.0 mg/1, and the ammonia (NH3~N) from 12.4 to 0.6 mg/1 after
      24 hours of exposure while the plant-free system demonstrated insigni-
      ficant reduction  of these components.  (AA)

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1020. Wolverton, B.C., R.M. Barlow and R.C. McDonald.  1975.  Application of
         Vascular Aquatic Plants for Pollution Removal, Energy, and Food Produc-
         tion in a Biological System.  NASA. NSTL. Bay St. Louis, MS.

         Vascular aquatic plants such as water hyacinths (Eichhorni a crassipes)
      (Mart.) Solms and alligator weeds (Al^ernanthera philoxeroides) (Mart.)
      Griesb., when utilized in a controlled biological system (including a
      regular program of harvesting to achieve maximum growth and pollution
      removal efficiency) may represent a remarkably efficient and inexpensive
      filtration and disposal system for toxic materials and sewage released
      into waters near urban and industrial areas.  The harvested and processed
      plant materials are sources of energy, fertilizer, animal feed, and human
      food.  Such a system has industrial, municipal, and agricultural applica-
      tions .  (AA)
1021. Wolverton, B. C., R. M. Barlow, and R. C. McDonald.  1976.  Application
         of Vascular Aquatic Plants for Pollution Removal, Energy, and Food
         Production in a Biological System, pp. 141-149.  In J. Tourbier and R.
         W. Pierson, Jr. (eds.), Biological Control of Water Pollution.  Univ.
         of Pennsylvania Press, Philadephia.

         This article describes the potential applications for wetlands as
      wastewater treatment systems.  A one acre water hyacinth wetland can
      remove N and P from sewage effluent, then be harvested to yield bio-gas
      and fertilizer.  Removal of pollutants such as phenol and heavy metals is
      possible also.  The water hyacinth has potential as a food source for
      cattle, and even for man.  (WE)
1022. Wolverton, B. C., and D. D. Harrison.  1974.  Aquatic Plants for Removal
         of Mevinphos From the Aquatic Environment.  J. Miss. Acad. Sci.,
         19:84-88.
1023. Wolverton, B. C., and R. C. McDonald.  1975a. Water Hyacinths and Alliga-
         tor Weeds for Removal of Lead and Mercury from Polluted Waters.
         National Aeronautics and Space Administration. Tech. Memorandum
         X-72723, National Space Tech. Laboratory, Bay St. Louis, MS.
1024.  Wolverton, B.  C., and R. C. McDonald.  1975b. Water Hyacinths and Alli-
         gator Weeds for Removal of Silver, Cobalt, and Strontium from Polluted
         Waters.  National Aeronautics and Space Administration. Tech. Memor-
         andum X-72727, National Space Tech. Laboratory, Bay St. Louis, MS.
1025.  Wolverton, B.  C.,  and R.  C.  McDonald.  1975c. Water Hyacinths for Upgrad-
         ing Sewage  Lagoons to  Meet Advanced Wastewater Treatment Standards:
         Part I. Tech.  Memo. TM-X-72729. National Aeronautics and Space Adminis-
         tration, National Space Technology Laboratories, Bay St. Louis, MS.

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         Water hyacinths were studied as a means of accomplishing final filtra-
      tion to remove nutrients, heavy metals/ and other chemicals from domestic
      wastewaters.   Biweekly grab samples from before and after saturating a
      secondary domestic sewage lagoon were compared with samples from a control
      lagoon free of water hyacinths.  The water hyacinth-covered lagoon had a
      surface area  of 0.70 acre with a total capacity of 1.5 million gallons/
      receiving an  inflow of 115,000 gallons per day from a 3.8 acre aerated
      primary sewage lagoon.  Retention times varied between 14 and 21 days
      depending on  the water hyacinth evapotranspiration rates.  The water hya-
      cinths functioned as an efficient and inexpensive final filtration system
      in a secondary domestic sewage lagoon during a three month test period.
      They reduced  the suspended solids/ biochemical oxygen demanding sub-
      stances / and  other chemical parameters to levels below the state stand-
      ards.  While  absorbing organics/ nutrients, and other chemical elements
      from sewage waste, they produced large quantities of plant material.  When
      grown in enriched sewage waste free of heavy metals, this biomass can be
      harvested and possibly processed into valuable feed products, organic fer-
      tilizer and soil conditioner, or methane gas and inorganic fertilizer.
      The volume of water hyacinths required depends onthe amount of sewage to
      be processes  and the desired purity of the final wastewater.
1026.  Wolverton, B.  C., and R. C. McDonald.  1976a. Don't Waste Waterweeds.
         New Scientist 67:318-320.

         Experiments conducted by the National Aeronautics and Space Administra-
      tion indicate  that water hyacinths (Eichhornia crassipes) have the ability
      to absorb organics, heavy metals, nutrients, and other chemical elements
      from wastewater under tropical and subtropical conditions while producing
      a large quantity of plant material.  This water hyacinth biomass/ when
      grown in sewage free of toxic metals, is a potential source of protein
      fertilizer, methane gas, and other valuable products.  Currently, a water
      hyacinth chemical waste filtration system consisting of a zig-zag canal
      330 meters long, 6.4 meters wide, and 0.8 meters deep is treating 95/000
      liters/day of  chemical and photo lab waste. On the basis of an analysis of
      raw sewage from small communities in South Mississippi/ a half hectare
      lagoon covered with water hyacinths with a minimum sewage retention time
      of 2 weeks is  estimated to be capable of purifying the daily wastes of
      1000 people.  An experimental water hyacinth lagoon has already reduced
      pollutant levels by 75-80%.
1027. Wolverton, B. C., and R. C. McDonald.  1976b. Water Hyacinths for Upgrad-
         ing Sewage Lagoons to Meet Advanced Wastewater Treatment Standards:
         Part II. Tech. Memo. TM-X-72730. National Aeronautics and Space Admin-
         istration, National Space Technology Laboratories, Bay St. Louis, MS.

         Field tests using water hyacinths as biological filtration agents were
      conducted in the Missisippi Gulf Coast Region.  The plants were installed
      in one single cell and one multiple cell sewage lagoon systems.  Water
      hyacinths demonstrated the ability to maintain BODs and total suspended
      solid (TSS) levels within the Environmental Protection Agency's (EPA) pre-
      scribed limits of 30 mg/1 BOD5 and 30 mg/1 TSS.

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         A multiple cell sewage lagoon system consisting of two aerated and one
      water hyacinth covered cells connected in series demonstrated the ability
      to maintain 8005 and TSS levels below 30 mg/1 year-round.  A water hya-
      cinth covered lagoon with a surface area of 0.28 hectare containing a
      total volume of 6.8 million liters demonstrated the capacity to treat
      437,000 to 1,893,000 liters of sewage influent from 2.65 hectares of
      aerated lagoons daily and produce an effluent that met or exceeded stand-
      ards year-round.  (AA)
1028. Wolverton, B. C., and R. C. McDonald.  1976c. Water Hyacinths, Eichhornia
         crassipes (Mart.) solms, a Renewable Source of Energy.  In; Capturing
         the Sun Through Bioconversion.  Center for Metropolitan Studies.
         Washington, DC.
1029. Wolverton, B.C., and R.C. McDonald.  1976d.  Water Hyacinths (Eichhornia
         cra s s ipe s) for Removing Chemical and Photographic Pollutants from Lab-
         oratory Wastewaters.  Tech. Memo. TM-X-72731, National Aeronautics
         Space Administration, Bay St. Louis, MS.  10 pp.
1030. Wolverton, B.C., and R.C. McDonald.  1977a.  Wastewater Treatment Utiliz-
         ing Water Hyacinths Eichhornia crassipes (Mart.) solms, pp. 205-208.
         In; Proceedings of the 1977 National Conference on Treatment and Dis-
         posal of Industrial Wastewaters and Residues, Houston, TX.
1031. Wolverton, B. C., and R. McDonald.  1977b. Compiled Data on the Vascular
         Plant Program, 1975-1977.  Rept. No. TM-79511.  National Aeronautics
         and Space Administration. Bay St. Louis, MS.  149 pp.

         The performance of a single cell, facultative sewage lagoon was signi-
      ficantly improved with the introduction of vascular aquatic plants.
      Water hyacinth (Eichhornia crassipes) was the dominant plant from April
      to November; duckweed (Lemna spp.) and (Spirodela spp.) flourished from
      December to March.  This 2 ha lagoon received approximately 475 cu m/day
      of untreated sewage and has a variable COD, loading rate of 22-30
      kg/ha/day.  During the first 14 months of operation with aquatic plants,
      the average influent BOD, was reduced by 95% from 110 mg/1 to an average
      of 5 mg/1 in the effluent.  The average influent suspended solids were
      reduced by 90% from 97 mg/1 to 10 mg/1 in the effluent.  Significant
      reductions in nitrogen and phosphorus were affected.  The monthly kjeldahl
      nitrogen for influent and effluent averaged 12.0 and 3.4 mg/1, respective-
      ly, a reduction of 72%.  The total phosphorus was reduced on an average of
      56% from 3.7 mg/1 influent to 1.6 mg/1 effluent.  (NT)
1032.  Wolverton,  B.  C.,  and R.  C.  McDonald.   1978a.  Bioaccumulation and Detec-
         tion of  Trace Levels of Cadmium in  Aquatic  Systems by Eichhornia
         crassipes.   Environmental Health Perspectives 27:161-164.

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         The water hyacinth (Eichhornia crassipes) may be used as a sensitive
      biological indicator for continuously trace quantities of toxic heavy
      metals in aquatic systems.  A river water system polluted with Cd was
      simulated while other factors of temperature, day-night cycle, water
      quality, and light intensity remained constant.  When the water hyacinth
      is maintained in river water containing 0.001 mg/1 of cadmium chloride,
      the plant's root system will concentrate this element at an average rate
      of 0.9, 1.4, and 3.0  g Cd/g root dry weight after 24, 48, and 72 hr
      exposure periods, respectively.  At a higher Cd concentration of
      0.01 mg/1, Cd was concentrated in the roots much faster to levels of 6.8,
      13.6, and 39.1  g/g root after 4, 8, and 24 hr exposure periods, re-
      spectively.  At initial  concentrations of 0.05 mg/1.  Cd, the roots con-
      tained 29.5, 48.8, and 156  g/g root following 4,8,  and 24 hr exposure
      periods, respectively.  During these same time intervals, the water
      hyacinth sorbed 56.7, 153, and 281  g/g root when the initial Cd concen-
      tration was increased to 0.10 mg/1.
         The water hyacinth tops can also assist in the monitoring process when
      Cd contamination levels  are 0.10 mg/1 and greater. At this initial Cd
      concentration, Cd is translocated into the tops.  After 8 hr, the tops
      averaged 1.1  g/g top.  After 24 hr, this concentration was increased to
      6.1  g/g top. (AA)
1033.  Wolverton, B.C.  and R.C. McDonald. 1978b.  Nutritional Composition of
         Water Hyacinths Grown on Domestic Sewage.  In;  Compiled Data on the
         Vascular Aquatic Plant Program:  1975-1977. NASA. NSTL Station. MS

         A nutrient analysis of water hyacinths grown in sewage wastewaters was
      conducted.  Crude protein averaged 32.9% dry weight in the leaves, where
      it was most concentrated.  The amino acid content of water hyacinth leaves
      was found to compare favorably with that of soybean and cottonseed meal.
      The vitamin and  mineral content of dried water hyacinths met or exceeded
      the FAO recommended daily allowance, in many cases.  It is concluded that
      in favorable climate zones, water hyacinths grown in enriched mediums,
      such as sewage lagoons, could potentially serve as a substantial dietary
      supplement or nutrient source.  (AA)
 1034.  Wolverton, B.C. and R.C. McDonald.  1978c.  Water Hyacinth Sorption Rates
         of Lead, Mercury, and Cadmium.  NASA. NSTL. Bay St. Louis, MS.

         Experiments were performed to test the ability of the water hyacinth
      (Eichhornia crassipes)  to remove lead, cadmium and mercury from solution
      both individually and in combination.  The plants were exposed to 10 ppm
      lead and one ppm each of cadmium and mercury for a period of 96 hours. The
      sorption of the heavy metals was monitored by periodic water sampling and
      plant tissue analysis at the termination of the experiment.  Results indi-
      cated no significant interactive effect among the three metals tested.
      Within one hour, water hyacinths removed approximately 65 percent of the
      lead, 50 percent of the cadmium and 65 percent of the mercury, whether the
      plants were exposed to these metals individually or in combination.
      Almost all of the heavy metals were concentrated in the root tissue of the
      plants, although there was some translocation, particularly of cadmium,
      into the upper plant parts.

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         These results are discussed in the light of previous investigations. It
      is concluded that the water hyacinth could be useful both as an agent for
      reducing heavy metal pollution and as an indicator or monitor of chronic
      metal contamination of aquatic systems.  (AA)
1035. Wolverton, B.C. and R.C. McDonald.  1978d.  Upgrading Faculative Waste
         Stabilization Ponds with Vascular Aquatic Plants.  In; Compiled Data on
         the Vascular Plant Program: 1975-1977.  NASA. NSTL Station, MS.

         The performance of a single cell, faculative sewage lagoon at NASA's
      National Space Technology Laboratories has been significantly improved
      with the introduction of vascular aquatic plants.  Water hyacinth
      (Eichhornia crasslpes) was the dominant plant from April to November;
      duckweed (Lemna spp.) and (Spi rodela spp.) flourished from December to
      March.  This 2 ha lagoon receives approximately 175 m^/day of untreated
      sewage and has a variable BOD5 loading rate of 22-30 kg/ha/day.
         During the first 14 months of operation with aquatic plants, the
      average influent 6005 was reduced by 95% from 110 mg/1 to an average of
      5 mg/1 in the effluent.  The average influent suspended solids were re-
      duced by 90% from 97 mg/1 to 10 mg/1 in the effluent.
         Although this lagoon was not harvested at a rate necessary to achieve
      maximum nitrogen and phosphorus removal, significant reductions in both of
      these nutrients were effected.  The monthly kjeldahl nitrogen for influent
      and effluent averaged 12.0 and 3.4 mg/1, respectively, a reduction of 72%.
      The total phosphorus was reduced on an average of 56% from 3.7 mg/1 influ-
      ent to 1.6 mg/1 effluent.  (AA)
1036. Wolverton, B. C., and R. C. McDonald.  1979a. Upgrading Facultative
         Wastewater Lagoons with Vascular Aquatic Plants.  3. Water Pollut.
         Control Fed. 51(2):305-313.

         The performance of a single-cell, faculative wastewater lagoon has been
      significantly improved with the introduction of vascular aquatic plants.
      Water hyacinth (Eichhornia crassipes) was the dominant plant from April to
      November; duckweed (Lemna and Splrodela spp.) flourished from December to
      March.  This 2-ha lagoon received approximately 475 m-^/d of untreated
      wastewater and had a 5-day biochemical oxygen demand loading rate of 22 to
      30 kg/ha d.  During the first 14 months of operation with aquatic plants,
      the average influent 5-day biochemical oxygen demand was reduced by 95%
      from 110 mg/1 to an average of 5 mg/1 in the effluent.  The average influ-
      ent suspended solids were reduced by 90% from 97 mg/1 to 10 mg/1 in the
      influent.  (AA)
1037.  Wolverton, B.C., and R.C. McDonald.  1979b.  The Water Hyacinth: From Pro-
         lific Pest to Potential Provider.  Ambio 8(1):2-9.

         The hardness and high productivity of the water hyacinth can be put to
      use in a controlled, freshwater environment to remove chemical pollutants
      from wastewater.  In the process of absorbing minerals and nutrients from

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      the water, the water hyacinth produces tremendous quantities of plant
      biomass which can be useful for feed, fertilizer or biogas. Great quanti-
      ties of pure, fresh water can also be recovered from water hyacinth sys-
      tems by covering them with greenhouses fitted with collection devices for
      capturing the water vapor that is normally lost through evapotranspira-
      tion.
         At present, water hyacinth systems are limited to freshwater systems in
      warm climates.  Floating greenhouses would be necessary to cover these
      systems if they were to be used in northern climates.  Another possibility
      of harnessing nature to clean up polluted waters in northern climates would
      be to survey the freshwater aquatic plants native to these regions which
      possess many of the characteristics which make the water hyacinth a good
      biological agent for pollution removal.  (AA)
1038.  Wolverton, B.C.  and R.C. McDonald.  1979c.  Energy From Aquatic Plant
         Wastewater Systems.  NASA.  NSTL. Bay St. Louis, MS.

         Water hyacinth (Eichhornia crassipes), duckweed (Spirodela sp. and
      Lemna sp.), water pennywort (Hydrocptyle ranunculoides), and kudzu (Puer-
      aria lobata) were anaerobically fermented using an anaerobic filter tech-
      nique that reduced the total digestion time from 90 days to an average of
      23 days and produced 0.14-0.28 m3 CH4/kg (dry weight) (2.3-4.5 ft3/lb)
      from mature filters.  The anaerobic filter provided a large surface area
      for the anaerobic bacteria to establish and maintain an optimum balance of
      faculative, acid-forming, and methane-producing bacteria.  Consequantly
      the efficiency of the process was greatly improved over prior batch fer-
      mentations.  (AA)
1039.  Wolverton, B. C., and R. C. McDonald.  1980. Water Hyacinth (Eichhornia
         crassipes) Productivity and Harvesting Studies. Economic Botany 34(1).

         Water hyacinth growth rates were monitored from May through October in
      two sewage lagoons with different nutrient loading rates.  The lagoon
      receiving the heaviest load sustained the highest average growth rates
      throughout the summer.  The lightly loaded lagoon averaged a 29% increase
      in weight per week over the six month period with the highest growth rate
      occurring during June with an average weekly weight gain of 71%.  The
      heavily loaded lagoon sustained an average growth rate of 46% per week for
      the same six month period with the highest measured growth rate of 73%
      increase in weight per week also occurring in June.  In addition, the per-
      formance of three harvesters was evaluated.  One harvester, consisting of
      a chopper and conveyor, was capable of picking up and chopping approxi-
      mately 2.3 t of plants per hour and delivering them to a waiting truck.
      The second harvester was a single 1.52 m (5 ft) wide conveyor, and the
      third one was a modified clamshell bucket attached to a dragline.  The
      average harvesting rate of each of these harvesters was approximately 9.3
      t of water hyacinths per hour.  (AA)

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1040. Wolverton, B. C., and R. C. McDonald.   1981.  Natural Processes  for
         Treatment of Organic Chemical Waste.  In Toxic and Hazardous  Sub-
         stances, a special publication of the Environmental Professional.

         Anaerobic filters and combination anaerobic filter/vascular aquatic
      plant systems were used to remove and biologically degrade phenol and
      m-cresol from contaminated river water.  The common reed  (Phragniites
      communis) and cattail (Typha latifolia.) were grown on top of two separate
      anaerobic filters.  Starting with approximately  100 mg/1 phenol
      solutions, the P. communis, T. latifglia, and plant-free anaerobic filter
      systems removed 93%, 83%, and 60%, respectively, of the phenol during the
      first 24 hours of exposure.  The P. communis and plant-free anaerobic
      filters removed 69% and 58% of m-cresol from 100 mg/1 solutions  after 24
      hours.  The results indicated that the phenol and m-cresol were  rapidly
      adsorbed on the filter surfaces and then assimilated and/or metabolized.
      Accurate determinations of the organics were made using gas chroma-
      tography.  The corresponding TOC, 6005, and COD of each sample were also
      reported.  (AA)
1041. Wolverton, B.C. and M.M. McKown. 1976. Water Hyacinths for Removal of
         Phenols from Polluted Waters.  Aquatic Botany, 2(1):191-201.

         A quantity of 2.75g dry weight of water hyacinth demonstrated the abil-
      ity to absorb 100 mg. of phenol/72 hr from distilled water, river water,
      and bayou water.  One ha of water hyacinth is potentially capable of
      removing 395 kg of phenol/72 hr from water polluted with this  chemical.
      (AA)
1042. Wolverton, B.C., R.C. McDonald and J. Gordon.  1975a.  Bio-Conversion of
         Water Hyacinths into Methane Gas: Part I.  National Aeronautics and
         Space Adminstration Tech. Memorandum X-72725, Natl. Space Tech.
         Laboratory, Bay St. Louis, MS.
1043. Wolverton, B. C., R. C. McDonald, and J. Gordon.  1975b. Water Hyacinths
         and Alligator Weeds for Final Filtration of Sewage.  Tech. Memo.
         TM-X-72724.  National Aeronautics and Space Administration, National
         Space Technology Laboratories, Bay St. Louis, MS.
1044. Wondrausch, J.  1969.  Phosphorus Sorption in Mucky-peat Soils.  Polish
         J. Soil Sci. 11:997-106.
1045. Wood, D.K., and G. Tchobanoglous.  1976.  Trace Elements in Biological
         Waste Treatment.  J. Water Pollut. Control Fed. 74:1933-1945.

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         The importance of trace elements for microbial growth and metabolism is
      well recognized.   However, their importance in the operation of biological
      waste treatment processes is not usually considered or understood fully.
      It is often assumed that the concentration of trace elements present in a
      wastewater will be sufficient for good treatment.  This assumption may not
      be valid, because the types and concentrations of trace elements in a
      wastewater vary with location and time.  Trace elements may also be
      removed from the  wastewater by various means, including adsorption and
      hydrogen sulfide  precipitation.  For these reasons, trace element defi-
      ciencies may occur, and treatment problems, including the occurrence of
      filamentous organisms, may result.  An argument supporting this thesis,
      along with an analysis of the role of trace elements in the activated
      sludge process, is presented.  Recommendations and alternatives for over-
      coming problems that may be caused by trace element deficiencies are
      offered.  (AA)
1046. Wood, J. M.  1974.  Biological Cycles for Toxic Elements in the Envir-
         onment.  Science.  183:1049-1052.

         In this article, the author describes some of the chemical and bio-
      chemical transformations of toxic elements in the environment, placing
      special emphasis on the role played by microorganisms.  He also shows that
      our present knowledge of the biological cycles of toxic elements enables
      us to predict the behavior of other toxic elements in the environment.
      (AA)
1047. Woodwell, G., J. Ballard, J. Clinton, and E. Pecan.  1976.  Nutrients,
         Toxins and Water in Terrestrial and Aquatic Ecosystems Treated with
         Sewage Plant Effluents.  Brookhaven National Laboratory, Upton, NY.

         The objective was to appraise the capacity of terrestrial and aquatic
      plant communities for absorbing and retaining nutrients and organic matter
      in sewage and for releasing 'clean* water.  The results showed the follow-
      ing:  (1)  Under normal circumstances, without the addition of water or
      nutrients in sewage, the flux of nutrients into the groundwater was great-
      est under the agricultural communities and least under the late succes-
      sional forest communities; (2) The highest concentrations of nutrients in
      the percolate of the untreated communities commonly occurred in the earli-
      est  stages of succession; (3) Treatment with sewage reduced the differ-
      ences in concentration in the percolate between the communities of the
      sere; (4) Calculation of the budgets of the various ecosystems showed that
      the forested stages accumulated the largest fractions of the inputs; (5)
      Appreciable changes occurred in the quality of percolate under the con-
      trolled communities over the period of the experiment; and (6) Present
      indications are that if sewage-treatment systems using natural communi-
      ties are to be devised, they will require combinations of aquatic and
      terrestrial systems.
1048. Woodwell, G. M.  1977.  Recycling Sewage Through Plant Communities.
         Amer. Sci. 65:556-562.

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         Recent research indicates that aquatic systems, especially marshes and
      ponds used in combination with terrestrial systems that benefit from
      irrigation, can be used to manage sewage effectively and inexpensively.
      An area of 50 acres containing a marsh associated with a shallow pond
      approximately the same size could effectively handle sewage for 10,000
      people.  In light of the economic and health repercussions that result
      from the current uncontrolled use of large water bodies for waste dis-
      posal-the New York Bight, Puget Sound, the Charles River, etc. - the
      transition toward closed, controlled systems, despite the large areas and
      careful design required, appears necessary and economical.  (EL)
1049. World Health Organization.  1973.  Reuse of Effluents:  Methods of
         Wastewater Treatment and Health Safeguards.  Tech. Rept. Series No.
         517.  Geneva, Switzerland.
1050. Wynn, S. L.  1979.  A Comparison of Data Collection and Methods Used to
         Monitor Impacts on Severely Disturbed Wetlands.  Ph.D. Diss. Univ. of
         Wisconsin.  Madison.  417 pp.
1051. Yip, S. W., and M. H. Wong.  1977.  The Effects of Sewage Sludge on the
          Growth Rate of Carp, Cyjgyinus carpip L. Environ. Pollution 14(2):
          127-132.

          The optimal quantity of sludge for rearing fish was investigated in
      the laboratory, Cvprinus carpio L. was chosen because it is one of the
      principal species reared locally in Hong Kong, and because it is a
      detritus feeder.  Carp reared in tap water to which sludge is added to
      give a concentration of 0.2%, or in sewage effluent, have higher growth
      rates than carp reared in tap water alone have.  (EL)
1052.  Yonika, D. A.   1979.  Effectiveness of a Wetland in Eastern Massachusetts
         in Improvement of Municipal Wastewater, pp. 91-100.  In R« K. Bastian,
         and S.  C.  Reed (eds.), Aquaculture Systems for Wastewater Treatment:
         Seminar Proceedings and Engineering Assessment.  EPA 439/9-80-006.
         U.S. Environmental Protection Agency, Office of Water Program
         Operations,  Municipal Construction Division.  Washington, DC.

         The quality  of water entering and leaving part of Great Meadows
      National Wildlife Refuge near Concord, Massachusetts, was studied.  The
      refuge receives chlorinated secondary effluent.  Nutrient removal effi-
      ciencies were measured over different seasons for each of the two wetland
      types present.   Low removal rates of about 0.5 pounds per acre per day of
      N and P were measured.  Removal rates were greatest during early spring,
      which corresponded to the period of most rapid vegetation growth.  (EP)

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1053. Yonika, D. A., and D. Lowry.   1978.  Bi-monthly summary reports:  Novem-
         ber-December 1977; March-April 1978;  May-June 1978;  September-October
         1978.  IEP, Inc., Wayland, MA.
1054.  Yonika,  D.,  and D.  Lowry.   1979.   Feasibility Study of Wetland Disposal
         of Wastewater Treatment Plant  Effluent.  Final report to the Massachu-
         setts Water Resources Commission, Division of Water Pollution Control.
         IEP,  Inc., Wayland, MA.  134 pp. (plus operations manual and biblio-
         graphy ).
1055. Yonika, D., and D. Lowry.  1980.  Wetland Disposal of Wastewater Treatment
         Plant Effluent.  IEP, Inc., Wayland, Mass.  October 24, 1977 to October
         24, 1978.
1056. Yount, J.L.  and R.A.  Grossman, Jr.  1970.  Eutrophication Control by Plant
         Harvesting.   J.  Water Poll. Contr.  Fed. 41:  173-183.
1057. Zahradnik, J. W., L. S. Turner, H. L. Levine, and C. Tucker.  1976.
         Evaluation of Proposed Man-Made Ponds for Food Production to Recover
         Values in Waste Heat and Solid Organic Sludges.  Publ. No. 65.  Water
         Resources Research Center, Univ. of Massachusetts, Amherst.  64 pp.

         An analysis was conducted to determine: (1) the potential value of
      using, simultaneously, thermal effluents and sewage sludges in aquatic
      cultures; (2) the technical and economic feasibility of utilizing multi-
      level aquatic cultures in the recovery of these wastes; and (3) data
      voids and uncertainties which are of importance in implementation of the
      system designed.   System inputs, potential system outputs, and potential
      subsystem characteristics were characterized and documented through a
      literature survey.  (NT)
1058. Zaim, M. and H.D. Newson, 1980.  Effects of Wastewater Spray Irrigation
         on Indigenous Mosquito Populations. Environmental Entomology 9(5):
         563-566.
1059. Zapatka, T.F., and R.W. Hann, Jr. 1976.  Evaluation of Safety Factors with
         Respect to Ocean Disposal of Waste Materials.  Sea Grant Publ. 77-202.
         Texas ASM University.  College Station, TX.  105 pp.

         Assessment of the risk and consequent environmental harm to the oceans
      is essential if ocean dumping is to be continued.   The available litera-
      ture, bioassay studies, and pertinent research concerning chronic effects
      and the risk they impose on the marine ecosystem was studied.  The corre-

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      lation between acute and chronic toxicities for known  hazardous  materials
      were investigated and defined.  The variables defined  to  be  of most
      importance are: stage of development, concentration  of toxicant,  time  of
      exposure, specific or non-specific reactions, lifetime of the compound,
      reactions with other chemicals, and bioaccumulation.   The ranges  of  ratios
      for different conditions and situations or at least  the ratios of acute to
      chronic toxicities for various classes of materials  were  determined.   (NT)
1060. Zeiders, K.E., 1975.  Stagonospora foliicola,  a Pathogen  of  Reed Canary
         Grass Spray-Irrigated with Municipal Sewage Effluent.  Plant  Dis.  Rep.
         59:779-783.
1061. Zeiders, K.E. and R.T. Sherwood.   1977.  Effect of Irrigation  with  Sewage
         Wastewater, Cutting Management, and Genotype on Tawny Blotch  of  Reed
         Canary Grass.  Crop Sci. 17:594-596.
1062. Zoltek, J., S. E. Bayley, F. L. Boyt.   1977.  Removal of Nutrients  from
         Treated Municipal Wastewater by Wetland Vegetation.  J. Water Pollut.
         Control Fed. 49(5 ):789-799.

         Secondary sewage effluent from Wildwood, Florida, is discharged  to  a
      mixed hardwood swamp,  and studies have  been carried out to assess the
      effectiveness of this method of disposal.  The results, summarized  in
      tables and graphs, indicate that this is a satisfactory alternative to
      tertiary treatment as regards removal of nutrients and bacteria, and the
      sewage effluent is, therefore, not a major contributor of pollution to
      Panasoffkee Lake, which is bordered by  the swamp.  The economic  value  of
      the swamp is estimated.  (AL)
1063. Zoltek, J., Jr., S. E. Bayley, T. J. Dolan, A. J. Herman, D.  A. Graetz,
         and N. L. Erickson.  1978.  Removal of Nutrient from Treated Municipal
         Wastewater by Freshwater Marshes.  Progress Report to the  City  of
         Clermont, FL. Center for Wetlands, Univ. of Florida, Gainesville.
         297 pp.
1064.  Zoltek, J., Jr., S. E. Bayley, A. J. Herman, L Tortora, and T. J.  Dolan.
         1979.  Removal of Nutrients from Treated Municipal Wastewater by
         Freshwater Marshes.  Final Rept. to the City of Clermont, FL.   Center
         for Wetlands, Univ. of Florida, Gainesville.  325 pp.
1065.  Zunarellilvandini, R. and A.M. Cognettivarriale.   1981.  Effects of Waste-
         water Effluents on a Polychaete Community in Littoral Sandy Bottoms.
         Cahiers de Biologic Marine 22(2):123-131.

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The most recent additions to the
bibliography are located on the
next pages.  All new citations
are alphabetized separately and
numbered in sequence with the
previous entries, beginning with
number 1066.   For the reader's
convenience, these new entries
have been inserted in proper al-
phabetical order into the main
entries by author and date.

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1066. Barten, J.M. 1983.  Nutrient Removal from Urban Stormwater by Wetland Fil-
         tration:  The Clear Lake Restoration Project.  Presented at the Fourth
         Annual Meeting of the Society of Wetland Scientists.  June 5-8, 1983.
         St. Paul/ Minnesota.

         Clear Lake is a 257 ha body of water located in southcentral Minnesota.
      It is a heavily utilized recreational lake which has become severely
      eutrophic due to the inflow of nutrient rich urban runoff from the adjacent
      City of Waseca, Minnesota.  In 1981, 50% of the hydraulic load and 55%  of
      the phosphorus load to the lake was diverted into a 21.4 ha peat marsh  on
      the northwest corner of Clear Lake.  A series of ditches and dikes were
      constructed in the marsh to retain stormwater until the phosphorus could be
      removed by percolation through the peat. The filtered water was then pumped
      into Clear Lake.  The filtration system was designed to allow for harvest-
      ing of vegetation in the marsh to prevent saturation of the phosphorus  ad-
      sorption sites in the peat.  In 1981, 73.3 x 104m^ of water were filtered
      through the system and 258.6 kg of phosphorus removed.  In 1982, 89.6 x 104
      m^ of water were filtered and 526.7 kg of phosphorus removed. The primary
      phosphorus removal mechanisms were physical entrapment and plant uptake.
      The total quantity of phosphorus removed in 1982 amounts to 40% of the
      average annual load to Clear Lake.  Mean orthophosphorus, total phosphorus
      and chlorophyll a concentrations in Clear Lake decreased significantly
      following diversion of stormwater to the marsh.  (AA)


1067. Bastian, R.K. and J. Benforado. 1983.  Waste Treatment:  Doing What Comes
         Naturally.  Technology Review 86(2):59-66.  Feb. - March.

         Wastewater and sludge are steadily growing in quantity and water pollu-
      tion remains a serious national concern.  These problems can be alleviated
      by viewing municipal wastewater and sludge as valuable resources.  Many
      communities can handle their sewage more economically through reuse by
      adopting "natural" treatment systems.
         The challenge is to learn how to manage natural ecosystems so that they
      can safely and effectively assimilate and recycle sewage wastes.  Better
      understanding of natural ecosystem functions will lead to development of
      "artificial" natural systems that capitalize on the capabilities of these
      same processes.
         A brief informational introduction is presented on the various methods
      of applying wastewater and sludge to land.  Current examples of operating
      systems are presented as well as a short discussion of the advantages/dis-
      advantages of each method.
         Aquatic treatment systems are also discussed in the same format of back-
      ground information,  current examples in operation,  and the relative attrac-
      tiveness of this wastewater treatment method.
         Federal policies  regarding alternative and innovative technologies are
      discussed along with data on the Federal money available for such grants
      and the results of the past few years of alternative and innovative tech-
      nology projects.
         Wide spread acceptance of natural treatment systems can be achieved  with
      more public education.   Also needed for success are good project design and
      management, more research on long term impacts of contaminants in a natural
      system, and appropriate regulations and guidelines.  Properly managed nat-
      ural ecosystems, while no panaceas, do offer an ecologically acceptable way
      to deal with pressing water pollution problems at reasonable cost.

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1068.  Benforado,  J.  1983.  Wetlands for Wastewater Treatment.  EPA Journal
         9(2):  14-15.
1069.  Black,  S.A,  I. Wile and G. Miller.  1981.  Sewage Effluent Treatment  in an
         Artificial Marshland.  Presented at the 1981 Conference of the Water
         Pollution Control Federation.  Detroit, Michigan.  Oct. 4-9,  1981.

         Sewage lagoons are a common means of wastewater treatment for smaller
      Ontario communities.  Frequently, the capacity of receiving waters to
      accept  effluent from such facilities is limited by inadequate dilution dur-
      ing summer low flow periods leading to nuisance proliferation of plants and
      algae,  with attendant widely fluctuating oxygen levels.  Storage of efflu-
      ent during periods of low flow requires large areas of land, and replace-
      ment of lagoons with mechanical treatment systems is very costly for  small
      municipalities.  Natural or artificial marshlands may provide a viable
      treatment alternative.
         To determine the potential of wetlands for year-round sewage treatment
      in Ontario,  an experimental study using an artificial marsh was established
      in 1979.  The study is designed to define the degree of pretreatment
      required prior to waste discharge to wetlands, maximum loading rates,
      required retention periods and depths of liquid to establish optimal  oper-
      ational limits for these types of systems.
         The  artificial marsh has been constructed at the site of the Listowel
      sewage  lagoon which consists of an aeration cell and two lagoons operated
      in series.  The channelized and open marshes are planted with cattails,
      Typha spp.
         Each system is capable of operating at flow rates of 0.5 to 2 times
      average design and at various depths and retention times.  Flows are  con-
      trolled and measured by V-notch and rectangular weirs and water  level
      recorders.  Routine parameters (i.e. nutrients, 8005, etc.) are  monitored
      on a weekly basis in all influent and effluent streams.
         This paper presents data on the first full year's operation of the arti-
      ficial  marsh, including loadings, removal efficiencies and effluent quali-
      ties of the various systems.
         Preliminary data suggest that artificial marshes have the capacity to
      improve the quality of partially treated wastewaters; the degree of
      improvement being dependent upon many factors including hydraulic loading,
      retention time, season and system configuration.   (AA)


1070. Brennan, K.M. (ed.).  1981.  The Effects of Wastewater Treatment Facilities
         on Wetlands in the Midwest.   (Draft Technical Report). Prepared under
         contract to the U.S. Environmental Protection Agency, Region V, by
         WAPORA, Inc.  (See also USEPA, 1983b, for final document.)


1071. Brennan, K.M., S.D. Bach and G.L. Seegert. In press.  Effects of Wastewater
         on Wetland Animal Communities.  In  Ecological  Considerations in Wet-
         lands Treatment of Municipal Wastewaters. Proceedings of a Workshop
         June 23-25, 1982.  University of Massachusetts,  Amherst, MA.  U.S. Fish
         and Wildlife Service and U.S. Environmental Protection Agency.

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            Little is known  of  the  effects  of  the  discharge of treated munici-ies
      pal wastewater on the  animal  communities of  freshwater wetland eco-
      systems.  The addition of nutrients,  suspended and dissolved solids,
      chlorine, heavy metals, and disease organisms  and the alterations of
      flow periodicity and rate, water  level,  pH,  and alkalinity constitute
      changes in environmental  conditions that could result in a wide range of
      changes in communities of invertebrates/ fish,  and other wildlife (am-
      phibians, reptiles, birds, and mammals). Because of  the potential
      impact and the lack of direct knowledge  of impacts, wetland use for
      wastewater treatment must be  carefully considered.  Most of the studies
      performed at wetland treatment sites  have focused on  changes in  water
      quality, vegetation, and  litter;  the  only studies of  wildlife at natural
      wetlands have been performed  in Florida, Michigan,  and Wisconsin.  Pre-
      liminary studies on wildlife  have also been  done at two volunteer wet-
      lands in Michigan.  Because so few studies have  been done on the pri-
      marily terrestrial animals inhabiting wetland  ecosystems which receive
      wastewater, the potential effects of  wastewater application must be
      identified by inference from  wildlife use of such treatment facilities
      as effluent lagoons, stabilization ponds, and  old field or forest land
      irrigated with wastewater.  On the positive  side,  the availability of
      nutrient-bearing water from a treatment  facility constitutes a potential
      resource that could be used to restore or maintain a  disturbed wetland,
      create an artificial wetland  to provide  additional habitat, or maintain
      a volunteer wetland (developed as a consequence of the growth of wetland
      plants in a flood irrigation  field or in a terrestrial plant community
      through which a wetland discharge passes), and  consequently,  to increase
      the diversity of wildlife habitat.  The  known  and anticipated effects of
      the discharge of treated  municipal wastewater  on the  animal communities
      of freshwater wetland  ecosystems  are  presented  in this paper according
      to three groups of animals present in wetland  ecosystems:

                        * Invertebrates
                        * Fish
                        * Wildlife  (amphibians, reptiles, birds,  and mammals).

      Information in the literature about the  effects  of wastewater on each of
      these groups is summarized and the major types  of impacts  identified  or
      postulated are presented  in the form  of  impact  factor trains.   In these
      diagrams, changes in the  physical and biological environment are indi-
      cated as potential causes of  subsequent  changes  in the animal communi-
      ties.  Effects on animal  health,  including potential  transmission of
      disease to humans,  are mentioned  briefly.  The  use of wastewater for  en-
      hancement of wildlife  habitat is  also discussed in the following paper.
      (AA)
1072.  Gosselink, J.G., and L. Gosselink.  In press.  The Mississippi  River
         Delta - A Natural Wastewater Treatment System.  In  Ecological
         Considerations in Wetlands Treatment of Municipal Wastewaters.
         Proceedings of a Workshop June 23-25, 1982.  University  of Massachu-
         setts, Amherst, MA.  U.S. Fish and Wildlife Service and  U.S.  Envi-
         ronmental Protection Agency.

         Wetlands have been shown to have considerable potential  for  waste-
      water treatment, but in only a few cases has it been possible to monitor
      the long-term effect of high nutrient loading rates on wetland  ecosys-
      tems (Kadlec and Kadlec 1979).

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         River deltas have evolved over millennia as the downstream receivers
      of  runoff into rivers.   Large rivers, especially, have high loading
      rates for sediments and sediment-related nutrients.  The Mississippi
      River delta is the largest wastewater treatment system in the United
      States;  it receives runoff from more than one-half of the U.S. land
      area.  Historically, as urban and industrial growth has occurred, the
      Mississippi River has become the conduit for elimination of wastes from
      a million households and thousands of industrial plants.
         The present day Mississippi River delta region is about 10,000  years
      old.   Its sediments contain a record of nutrient deposition that goes
      back  to the end of the last glaciation.  The hydrogen bomb fallout
      product Celsium-137(^'Cs) has labelled the sediments and allows us
      to  examine the last 30 years in detail. What can we learn from this
      record?  Is the delta a useful analog for a municipal overland flow
      system?  If it is, what are the long-term characteristics of the delta,
      and what conditions make its activity effective?  To answer these
      questions, we examined the nutrient concentrations and loading rates of
      river water flowing into the delta, and the nutrient retention ability
      of  several different kinds of delta environments.  (AA)
1073.  Grimes, D. In press.  Microbiological Studies of Municipal Waste Release
         to Aquatic Environments.  In  Ecological Considerations in Wetlands
         Treatment of Municipal Wastewaters.  Proceedings of a workshop, June
         23-25,  1982.  University of Massachusetts, Amherst MA.  U.S. Fish and
         Wildlife Service and U.S. Environmental Protection Agency.
1074.  Guntenspergen, G.R., and F. Stearns. In press. Ecological Perspectives
         on Wetland Systems.  In  Ecological Considerations in Wetlands
         Treatment of Municipal Wastewaters.  Proceedings of a Workshop June
         23-25, 1982.  University of Massachusetts, Amherst, MA.  U.S. Fish
         and Wildlife Service and U.S. Environmental Protection Agency.

         Wetlands have long been used as sites for domestic sewage discharge,
      but the studies needed for an ecosystem-based evaluation are few and
      recent (e.g., Boyt et.al. 1977); Ewel and Odum 1979; Tilton  and Kadlec
      1979; Valiela, this volume; Guntenspergen et.al. 1980;  Mudroch and
      Capobianco 1979; Deghi et.al. 1980; Dolan et.al. 1981).  To date, most
      wetland studies have emphasized water quality effects to the exclusion
      of other impacts.  The major concern has been the ability of the
      wetlands to significantly improve the quality of  the effluent being
      discharged.  We believe that examination of the ecological impacts of
      wastewater application is essential and is long overdue.  It is unrea-
      listic to assume that there will be no ecological changes.  In an
      earlier review  (Guntenspergen and Stearns 1981), we concluded that
      biological changes are associated with nutrient  addition to wetlands
      and that these changes are manifested at the ecosystem level.  A number
      of studies have indicated that nutrient addition through effluent
      discharge enhanced biomass production and, at the same time, certain
      species were eliminated by alterations in  the physical environment or
      by competition.  (AA)


1075. Hammer, D.E. and R.H. Kadlec.   1983.  Design Principles for Wetlands
         Treatment Systems.  Project Summary.  EPA-600/S2-83-026.  Robert S.
         Kerr Environmental Research Laboratory.  Ada, Oklahoma.  May.  (See
         also Hammer, D.E. et al., 1983 for complete version of this article.)

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         Published data pertaining to the treatment of wastewater  by wetland
      irrigation have been assembled and analyzed to begin identifying general
      principles for the successful design of wetland facilities.   Sources  of
      operating data have been tabulated.  Performance is roughly  correlated
      with overall systems features, but cannot be predicted on  the current
      basis.   Existing compartmental models require more detailed  information
      than does or will exist; thus a simplified compartment model is pre-
      sented.
         Water quality is controlled by rapid processes related  to water move-
      ment, mass transport to other commpartments, and consumption kinetics.
      Thus, wetland hydrology is fundamental to the analysis of  water quality
      improvement.  The ultimate fate of nutrients and contaminants is deter-
      mined by sedimentation, biomass production and harvest,  soil and micro-
      bial processes.  Required wetland area depends on effluent quality, eco-
      system type and age, and hydraulic regime.  These questions  can be ad-
      dressed in terms of a mass transport model for the zone  of rapid removal
      and a "saturation" model for the expansion of a zone of  stabilized
      activity about the discharge point.  Material balances,  considering only
      long-term consumption mechanisms for nutrients and other pollutants,
      determine the useful life and ultimate performance of a  wetland system.
         Operational techniques and the use of constructed wetlands are also
      considered.  The economics of wetland treatment are discussed.   (AA)
1076.  Hammer, D.E. et.al.  1983.  Design Principles for Wetland Treatment Sys-
         tems.  University of Michigan - Ann Arbor.  National Technical  Infor-
         mation Service No. PB83-188722.  April. (See also Hammer, D.E.  and
         R.H. Kadlec, 1983, for EPA Project Summary version of this  article.)
         Published data pertaining to the treatment of wastewater by wetland
      irrigation have been assembled and analyzed to begin to identify  general
      principles for successful design of wetland facilities.  Sources  of
      operating data have been tabulated.  Performance is roughly correlated
      with overall system features, but cannot be predicted on the  current
      basis.   Existing compartmental models require more detailed information
      than does or will exist; thus a simplified compartment model  is pre-
      sented.
         Water quality is controlled by rapid processes related to  water move-
      ment,  mass transport to other compartments, and consumption kinetics.
      Thus,  wetland hydrology is fundamental to the analysis of water quality
      improvement.   The ultimate fate of nutrients and contaminants is  deter-
      mined by sedimentation, biomass production and harvest, and soil  and
      microbial processes.  Required wetland area depends on effluent quality,
      ecosystem type and age, and hydraulic regime.  These questions can be
      addressed in terms of a mass transport model for the zone of  rapid re-
      moval,  and a "saturation" model for the expansion of a zone of stabil-
      ized activity about the discharge point.  Material balances,  considering
      only long-term consumption mechanisms for nutrients and other pollutants
      determine the useful life and ultimate performance of a wetland system.
         The  selection of natural sites and the physical facilities associated
      with wetland treatment are discussed.   A possible protocol for site re-
      view is  presented.  An appendix of existing wetland sites used for
      wastewater treatment is included.  Operational techniques and the use of
      constructed wetlands are also considered.  Finally, wastewater impacts
      on wetlands and the economics of wetland treatment are discussed.  (AA)

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1077. Heliotis, F.D. 1981.  Wetland Systems for Wastewater Treatment:  Opera-
         ting Mechanisms and Implications for Design.  University  of Wisconsin
         -Madison.  Master's Thesis.  103 p.

         The goal of this study is to provide a working knowledge  of the
      mechanisms related to the interactions of secondarily  treated wastewater
      with natural wetlands and to suggest an approach to the rational design
      of wetland treatment systems.
         A systematic review of nutrient cycling studies in  wetlands resulted
      in the construction of a conceptual model of nutrient  dynamics.   The
      following needs were identified: 1) Quantification of  hydrological  re-
      lationships emphasizing the contribution of different  water  sources to
      the nutrient budget of the wetland; 2) Mass-balance studies  of wetland
      processes especially decomposition, adsorption phenomena, and peat
      accumulation; 3) Studies on the role of periphyton in  nutrient uptake;
      and 4) Studies on the nutritional ecology of wetland macrophytes.
         The response of different wetland types to wastewater addition was
      reviewed with emphasis on mechanisms for the removal of nutrients.  It
      was concluded that the major long-term mechanism for the removal of
      phosphorus is incorporation to peat whereas, denitrification is  respon-
      sible for most of nitrogen removal.  The best nutrient removals  were
      observed in wetlands with thick peat soils and in nutrient limited
      swamps.  Wetlands receiving energy subsidy from tides  or waves do not
      seem to effectively treat wastewater.  Environmental alterations due to
      the addition of wastewater include decreasing diversity and  stability
      with a shift of the trophic relationships towards simpler food chains.
      It is recommended that an evaluation of the initial wetland  quality
      should be performed in order to avoid using high value wetlands.
         The following steps were suggested for an approach  to the rational
      design:  1) Identification of objectives; 2) Identification  of time-
      frame; 3) Assessment of sensitivity to environmental perturbation;  4)
      Identification of operating mechanisms; and 5) Identification of manage-
      ment options.  A discussion of the time element revealed the need for
      calculation of turnover times of various system components.
         The lack of information on many aspects of wetland  ecology especially
      as related to long-term impact of wastewater is considered as a  suffi-
      cient reason for avoiding their use.  If, however, the options are
      limited,  it is recommended that only degraded wetlands should be used
      and the purpose of management should be to integrate the practice to the
      overall functions of the landscape.  (AA)
1078.  Heliotis, F.D. and C.B. DeWitt.  1983.  A Conceptual Model  of Nutrient
         Cycling in Wetlands Used for Wastewater Treatment:  A Literature
         Analysis.  Presented at the Fourth Annual Meeting of the Society  of
         Wetland Scientists.  June 5-8, 1983.  St. Paul, Minnesota.

         A conceptual model of nutrient dynamics in wetlands use  for waste-
      water treatment provided the basis for reviewing the literature  on the
      subject.  Papers were selected in order to describe the storages and
      transfers of the conceptual model.  This approach serves to develop  an
      understanding of wetland systems behavior and allows easy identifica-
      tion of gaps in the literature and of research needs.  The  response  of
      different wetland types to wastewater addition was evaluated with empha-
      sis on the mechanisms of nutrient removal.  The concensus of the liter-
      ature is that incorporation into peat is the major long-term mechanism

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      for phosphorus removal; that ecological changes reflect the  response  of
      the system to nutrient enrichment; and that wetland quality  should  be
      evaluated before the application of wastewater. It is recommended that
      an approach to the design of wetland wastewater treatment systems should
      include identification of objectives, time frame, operating  mechanisms,
      management options, and assessment of sensitivity to environmental  per-
      turbation.  (AA)
1079. Hodson, E., A.E. Maccubbin, R. Benner, and R.E. Murray. In press.  Micro-
         bial Transformation of Detrital Carbon in Wetland Ecosystems: Effects
         of Environmental Stress.  In  Ecological Considerations of Wetlands
         Treatment of Municipal Wastewaters.  Proceedings of a Workshop June
         23-25, 1982.  University of Massachusetts, Amherst MA.  U.S. Pish  and
         Wildlife Service and U.S. Environmental Protection Agency.
      Hyde, H.C., R.S. Ross and F. Demgen.  In press.  Technical Assessment  of
         Wetlands for Municipal Wastewater Treatment.  USEPA Office of
         Research and Development, Municipal Environmental Research Lab.
         Cincinnati.
1080.  Kadlec,  J.A.  In press.  Wetlands, Wastewater, and Wildlife.  In  Ecolog-
         ical Considerations in Wetlands Treatment of Municipal Wastewaters.
         Proceedings of a Workshop June 23-25, 1982.  University of Massachu-
         setts,  Amherst MA.   U.S. Fish and Wildlife Service and U.S. Environ-
         mental  Protection Agency.
         Wetlands are noted for the diversity and abundance of wildlife that
      Inhabits them.   Indeed,  it was their importance to wildlife, notably
      duck and geese, that led to the first (and, for a long time, only) ef-
      forts to preserve wetlands and marshes.   More recently, other values of
      wetlands have received increased attention.  Of particular concern to us
      here is the potential use of wetlands for wastewater treatment,  wildlife
      interests view this possibility with mixed motions — having battled so
      long for wetlands preservation, they welcome any help they can get in
      "selling" the need for wetlands, but they are concerned that this use not
      destroy the very values  they seek to preserve.
         Data on the effects on wildlife of wastewater inputs to wetlands are
      distressingly scarce.  As a consequence,  the approach adopted herein is
      to examine the documented and predicted effects of wastewater on wet-
      lands,  and then to interpret those effects in terms of their potential
      impact on wildlife.  For convenience, I will categorize wastewater
      effects on wetland ecosystems in five major categories:  1) primary
      production, 2)  plant community structure, 3) water regime, 4) pathogens,
      and 5)  toxic chemicals.   These are not mutually exclusive and there are
      important interactions among categories,  but each category serves to
      focus attention on a particular set of concerns.
         Before commencing a consideration of each of the major effects, a
      comment about the ubiquity of change in wetlands is in order.  Natural,
      pristine wetlands are dynamic and undergo change at various rates, yet
      many are essentially permanent features  of the landscape.  For example,
      prairie potholes seem to change in a cyclic fashion with patterns of
      drought,  with a time scale on the order  of 5-10 years (van der Valk and

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      Davis 1978),  yet they have persisted over a vast area of the northern
      prairies since the last glaciation, approximately 10,000 years ago.
      Against that background of natural change, our knowledge of wetland
      ecosystems strongly indicates that the discharge of wastewater into a
      wetland ecosystem will produce changes, perhaps dramatic, perhaps subtle,
      in that system.  It might very well change one kind of wetland into a
      different kind.  Both kinds may well be "natural" in the sense that
      equivalents are found elsewhere as essentially undisturbed systems.  A
      part of this change quite  probably will be a change in wildlife.  Some
      species will increase, others decrease.  In and of itself, such change is
      neither bad nor good, but becomes so only in the context of human values
      and the  relative commoness or rarity of these ecosystems in the land-
      scape/  usually at the regional, state, or national level.  (AA)
1081.  Kadlec,  R.H.  1983.   The Bellaire Wetland:  Wastewater Alteration and Re-
         covery.   Presented at the Fourth Annual Meeting of the Society of Wet-
         land Scientist.   June 5-8, 1983.  St. Paul, Minnesota.

         The village of Bellaire, MI has discharged wastewater from their sta-
      bilization lagoons  to a 18 hectare wetland for ten years at about 30 mil-
      lion gallons per year.  For the last seven of those years, studies of
      hydrology,  water quality, vegetation and soils have been conducted.  Dis-
      charges terminated in 1981, with a resultant recovery of the ecosystem.
      During irrigation,  wastewater parameters were dramatically improved by
      waste treatment standards/ but not by receiving ecosystem standards.
      Mass balances for water and chloride agreed reasonably well.  All sur-
      face inflows and outflows and precipitation were measured; and evapo-
      transportation was  calculated from climatic data.  With well-defined
      hydrology,  mass balances were possible for nutrients.  Nitrogen, phos-
      phorus,  and suspended solids were retained or removed in large measure.
      After irrigation, no significant release occurred.  Vegetation suffered
      major damage in the immediate vicinity of the discharge; trees were up-
      rooted and new species invaded.  Heavy growths of fungi and bacteria
      were noted in the immediate area of wastewater application.


1082.  Kadlec,  R.H.   In press.  Aging Phenomena in Wastewater Wetlands.  In
         Ecological Considerations in Wetlands Treatment of Municipal Waste-
         waters.   Proceedings of a Workshop June 23-25, 1982.  University of
         Massachusetts, Amherst, MA.  U.S. Fish and Wildlife Service and U.S.
         Environmental Protection Agency.


1083.  Kadlec,  R.H.  and D.E. Hammer.  1982.  Wetland Utilization for Management
         of Community Wastewater - 1981 Operations Summary.  Houghton Lake Wet-
         lands Treatment Project.  Report to the National Science Foundation,
         Grant ENV-23868.  Wetlands Ecosystem Research Group, College of Engi-
         neering, The University of Michigan, Ann Arbor MI, 69 p.


1084.  Kadlec, R.H. and D.E. Hammer. 1983.  Wetlands Utilization for Management
         of Community Wastewater - 1982 Operations Summary.  Houghton Lake Wet-
         lands Treatment Project.  Report to the National Science Foundation.
         Wetlands Ecosystem Research Group, College of Engineering, The Univer-
         sity of Michigan, Ann Arbor MI, 67 p.

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      Kusler, J.A.  1980.  Regulating Sensitive Lands.  Ballinger Pub.  Co.
         Cambridge, MA.  248 p.
      Kusler, J.A.  1983.  Our National Wetland Heritage  A Protection
         Guidebook.  Environmental Law Institute.  Washington, D.C.   168 p.
1085. Monge, D. 1978.  Bibliography of Wetland Values.  School of Forestry.
         State University of New York, Syracuse.
1086. Nichols, D.S. 1983.  Capacity of Natural Wetlands to Remove Nutrients
         from Wastewater.  Journal Water Pollution Control Federation.  55(5):
         495-505.

         Wastewater, even after secondary treatment, is a major source  of
      nutrients that can cause eutrophication of lakes and streams and  deter-
      ioration of water quality.  Conventional advanced wastewater treatment
      typically requires large capital investments and consumes large amounts
      of energy.  Therefore, interest is increasing in the use of natural wet-
      lands as a simple and energy-efficient means of removing nutrients from
      wastewater.  Wastewater effluent has been applied to many types of nat-
      ural wetlands from Florida to Canada's Northwest Territories.  In all of
      these studies, some nitrogen and phosphorus was removed from the  waste-
      water as it flowed through the wetland.  However, a more quantitative
      assessment is needed of the capacities and limitations of wetlands to
      removal nutrients.  The purpose of this paper is to review the mechanisms
      by which wetlands remove wastewater nutrients and to synthesize from data
      in the literature a model of the relations between wastewater nitrogen
      (N) and phosphorus (P) application rates and efficiency of N and  P
      removal by wetlands.  (AA)


1087. Odum, H.T., In press.  Water Conservation and Wetland Values.  In
         Ecological Considerations in Wetlands Treatment of Municipal
         Wastewaters.  Proceedings of a Workshop June 23-25, 1982.  University
         of Massachusetts, Amherst, MA.  U.S. Fish and Wildlife Service and
         U.S. Environmental Protection Agency.

         It might be said that the hydrological cycle is the ATP of the bio-
      sphere, because of its role in organizing and driving ecosystems.  Wet-
      lands are one manifestation of landscape organization by water.   Whereas
      photosynthetic productivity is sometimes used as a basis for evaluating
      ecosystems, other kinds of work involving the hydrologic cycle may be
      more important in wetlands.  What do wetlands do with water, and  how is
      this related to energy and to regional value?  The answers to these ques-
      tions may affect the way we manage wetlands, preserve them, or generate
      new ones in making a better mosaic of humanity and nature.  (AA)
1088.  Reed, S.C. and R.K. Bastian.  In press.  Wetlands for Wastewater Treat-
         ment — An Engineering Perspective.  In  Ecological Considerations
         in Wetlands Treatment of Municipal Wastewaters.  Proceedings of a
         Workshop June 23-25, 1982.  University of Massachusetts, Amherst,
         MA.  U.S. Fish and Wildlife Service and U.S. Environmental Protec-
         tion Agency.

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   The capability of wetlands to renovate wastewater,  as  well as tolerate
effluent discharges, has been demonstrated  in a  variety  of  natural wet-
land types across the country, as well  as in  "artificial" or constructed
wetlands.  This has been well documented elsewhere  in  a  number of state-
of-the-art conference and workshop proceedings  (Stowell  et  al.,  Hantzsche,
this volume) and special project reports, such  as:  a 1978 review by
Duffer and Moyer, the proceedings of  a  multi-agency-sponsored seminar
held at the University of California-Davis  in September  of  1979  (Bastian
and Reed 1980), and a literature review of  the  vegetative and hydraulic
processes responsible for wetlands removal  of selected pollutants from
storm water runoff and treated municipal wastewater by Chan et al.
(1981).
   Under the right conditions, wetland  systems  can  achieve  high removal
efficiencies for BOD, suspended solids, trace organics and  heavy metals.
They have considerable potential as a low-cost,  low-energy  technique for
upgrading wastewater effluents (especially  for  smaller communities loca-
ted in areas where natural wetlands abound  or ample opportunity for the
construction of new wetlands exists).   The  concept  has been shown to be
viable and qualifies under current EPA  definitions  as  an innovative tech-
nology.  Projects which incorporate such technologies  are eligible for
more federal construction grants assistance than those projects which re-
ly solely on conventional technologies.  However, the  specific factors
responsible for such high treatment levels  are  not  clearly  understood at
present.  Optimum cost-effective criteria applicable throughout the U.S.
are not now available for routine design of wastewater treatment systems
involving wetlands as a viable part of  the  treatment facility.
   The use of constructed wetlands shows great  promise for  more general
application because, potentially, they  are  more  reliable and involve less
risk of adverse environmental impact  because  of  better process control.
The potential for a more widespread and routine  use of wetland systems
(particularly the constructed type) seems high  — if and when reliable,
cost-effective, engineering criteria  are made available.   While consider-
able information about these systems  is already available,  the scientific
uncertainties over the long-term impacts  (beneficial as  well as detrimen-
tal) of applying municipal wastewater to managed wetland systems can only
be clarified through more experience  with operational  systems.  A closely
coordinated, multidisciplinary approach involvling  the cooperative
efforts of experienced engineers, ecologists, biologists, hydrologists,
etc. will be required to adequately address the full range  of ecological
and related issues that must be addressed when  designing wetland treat-
ment systems.
   The engineering design of a wastewater treatment system is based on a
predictable or an assumed set of conditions.  The successful performance
of that system is then based upon the maintenance of those conditions
throughout the useful life of the system — for most municipal wastewater
treatment facilities, this is 20 years• When the conditions cannot be
guaranteed, the engineer incorporates a safety  factor and overdesigns the
system to compensate for the uncertainty.   Most of  these aspects are more
or less under control during the design and operation of a constructed or
"artificial" wetland, so the safety factors for this concept can be rela-
tively low.  These systems can then reliably  treat  a relatively large
volume of wastewater in a confined space, and the design criteria used
can be extrapolated for use in the design of  similar systems in the same
climatic zone.  In natural wetlands,  the hydrologic regime is difficult
to predict and often impossible to maintain.  So a  very  large safety fac-
tor must be incorporated to insure reliable treatment under all condi-

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      tions.  As a result, the wastewater loading will tend to be relatively
      low and the required wetland area relatively large.  Since the hydrologic
      regime of the natural setting is also subjected to local influences, the
      extrapolation of design criteria for one natural wetland system to
      another setting will be difficult.  (AA)
      Richardson, B. (ed).  1981.  Selected Proceedings of the Midwest Con-
         ference on Wetland Values and Management.  June 17-19.  St. Paul,
         Minnesota.  Minnesota Water Planning Board; Water Resources Research
         Center, University of Minnesota; Upper Mississippi River Basin
         Commission; and Great Lakes Basin Commission.
1089. Richardson, C.J. and D.S. Nichols. In press.  Ecological Analysis of
         Wastewater Management Criteria in Wetland Ecosystems.  In  Ecological
         Considerations in Wetlands Treatment of Municipal Wastewaters.
         Proceedings of a Workshop June 23-25, 1982.  University of Massachu-
         setts, Amherst, MA.  U.S. Fish and Wildlife Service and U.S.
         Environmental Protection Agency.

         Considerable interest has been focused on the use of wetlands to treat
      secondary municipal sewage effluent further (Valiela et.al. 1975; Tilton
      et.al. 1976; Tilton and Kadlec 1979; Odum and Ewel 1980; Lyons and Ben-
      forado 1981).  Reports on the effectiveness of wetlands in removing
      nitrogen and phosphorus from effluent have been encouraging (Richardson
      et.al. 1976; Tourbier and Pierson 1976; Kadlec 1979b; Kadlec and Tilton
      1979; Whigham and Bayley 1979), but questions persist about sorption
      capacity, uptake rates (Richardson et.al. 1978), and long-term capacities
      of wetland ecosystems to remove nutrients (Richardson 1981; Nichols
      1983). This paper presents a series of ecological management criteria
      that should be addressed prior to the decision to use any wetland eco-
      systems for treatment of secondary municipal effluent. These criteria
      include the value of the effluent as a resource, the capacities and
      limitations of wetlands to accomplish wastewater treatment, wastewater
      management objectives, wastewater suitability for wetland discharge, and
      wetland values.  Also presented are discussions of wetland hydrology,
      productivity, cycling of nutrients and heavy metals, and estimates of
      efficiencies of wastewater nutrient removal by wetlands and the wetland
      area needed for specific levels of nutrient removal.  (AA)


1090.  Rusincovitch, F. In press.   Use of Wetlands for Wastewater Treatment and
         Effluent Disposal:  Institutional Constraints.  In  Ecological Consid-
         erations in Wetlands Treatment of Municipal Wastewaters.  Proceedings
         of a Workshop June 23-25, 1982.  University of Massachusetts, Amherst,
         MA.  U.S. Fish and Wildlife Service and U.S.  Environmental Protection
         Agency.

         The Environmental Protection Agency's Office of Federal Activities
      (Policy and Procedure Branch and 404 Branch) and the Office of Water Pro-
      grams, in conjunction with the U.S.  Fish and Wildlife Service, are con-
      sidering the use of wetlands to treat municipal wastewater.  The impetus
      for such use is that many small communities have found that "high tech-
      nology" conventional municipal sewage treatment systems are not well
      suited to their limited needs.

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         The primary reason that such conventional municipal treatment  systems
      are often not appropriate for small communities is that they  use  sophisti-
      cated biological and chemical processes to remove biochemical oxygen  de-
      mand (BOD),  suspended solids, and nutrients.  Such systems are  relatively
      expensive to operate, especially on a scale suited to small communities
      and, for the most part, require at least one full time, highly  trained
      operator to work properly.  The advantage for larger communities  is that
      higher technology treatment plants can remove pollutants from municipal
      waste more rapidly and require much less land area than the less  techni-
      cal, less expensive, more "natural" systems, such as stabilization ponds.
      (AA)
1091.  Shiaris, M.P. In press. Public Health Implications of Sewage Applications
         on Wetlands:   Microbiological Aspects.  In  Ecological Considerations
         in Wetlands Treatment of Municipal Wastewaters.  Proceedings  of  a
         Workshop June 23-25, 1982.  University of Massachusetts, Amherst, MA.
         U.S.  Fish and Wildlife Service an* U.S. Environmental Protection
         Agency.

         With an ever expanding population, demand for water  for urban agricul-
      tural, and industrial use is growing.  At the same time, the volume of
      biological and chemical wastes which enter our waters is also  increasing.
      The impending dilemma requires a switch to increased and more  efficient
      water recycling.  The potential use of wetlands as wastewater  treatment
      sites is a promising approach (U.S. EPA 1979, Kadlec and Tilton  1979);
      hence, the subject of this workshop.
         Any future application of sewage to wetlands must be free of  unreason-
      able risks to public health.  As a rule, untreated municipal wastewater
      contains many components which pose a threat to the well-being of wild-
      life and humans.  Pathogens from human and animal feces, organic toxins,
      industrial waste, heavy metal, and pesticides are present in varying
      amounts in all municipal sewage.  A variety of specific treatments  to
      attain a satisfactory water effluent are available. The projected use of
      the recycled wastewater and the potential for human contact will dictate
      which treatment and the degree of treatment necessary to reduce  the
      health risk (Shuval 1982).
         Microorganisms are of paramount importance in any public health  con-
      sideration of wastewater.  Clearly, the direct hazards  of microorgan-
      isms as agents of disease in wastewater are well recognized if not  fully
      understood.  The role of microorganisms as agents of biotransformations,
      however, is not as well recognized and certainly not as well understood
      relative to public health.  In a wetlands treatment site, the  microbial
      component may actively detoxify hazardous chemicals and, conversely, may
      transform or "activate" some relatively nontoxic chemicals to  more  toxic
      forms.
         The present purpose is to highlight briefly the potential health risks
      of microorganisms and microbial activities in sewage wastewater.  Rela-
      tively little information is available on the public health hazards that
      may be encountered by applying wastewater to wetlands.  On the other
      hand, there is considerable information on land application health
      aspects which have been reviewed elsewhere (Lance and Gerba 1978; Kowal,
      in press; Pahren et.al. 1979).  Regulatory authorities  postulate that the
      health risks for wetland systems will not be greater than the  hazards
      encountered in conventional wastewater treatment, assuming that  materials
      will not be harvested for human consumption (U.S. EPA 1979).   Therefore,

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      while most of the work discussed herein was not conducted in wetland
      systems,  it will be assumed that the health risks discussed are
      applicable  to wetlands as well.  (AA)


1092.  Sutherland, J.C. In press.  Wetland-Wastewater Economics.  In  Ecological
         Considerations in Wetlands Treatment of Municipal Wastewaters.  Pro-
         ceedings of a Workshop June 23-25, 1982.  University of Massachusetts,
         Amherst, MA.  U.S. Fish and Wildlife Service and U.S. Environmental
         Protection Agency.

         In the mid-1970's, there was a great need in Michigan for economical,
      post-secondary wastewater treatment, including phosphorus removal.
      Wetland application for removal of phosphorus from this community's
      stabilization pond effluent seemed an affordable alternative.  By early
      1976, four years of research under the direction of Dr. Robert Kadlec of
      the University of Michigan at the Porter Ranch peatland near Houghton
      Lake, Michigan indicated excellent renovation of wastewater and nitrogen
      species by wetland application (Kadlec  et.al. 1976).
         If it had not been for Natural Science Foundation (NSF) support, a
      full-scale wetland treatment project at Houghton Lake might never  have
      been developed.  Nothing like it had been done before in Michigan.  The
      U.S.  EPA had never funded design and construction of a wetland treatment
      project through the Municipal Construction Grants program, and state and
      federal review agencies had understandable concerns for the integrity of
      this pristine natural wetland. The factors that brought success to the
      project were excellent P-removal potential, prospects for net positive
      environmental responses, an informed local populace, and great projected
      savings in wastewater treatment costs.  The construction cost for the
      wetland at Houghton Lake was projected, in 1976, to be $600,000.  The
      construction of the upland irrigation alternative was projected at $1.1
      million or 83% higher.  The construction of the wetland wastewater
      facilities, in fact, cost approximately $400,000 (1978 dollars).
         By early 1976, the NSF project officer, Dr. Edward Bryan, thought it
      time to develop the general economic feasibility picture.  Williams and
      Works, Inc. was selected to begin such a study, limited to Michigan
      (Sutherland 1977).   At that time,  we have more questions than informa-
      tion.  One of the questions was what kind of pretreatment assumption
      should be made.  It seemed reasonable to think of secondary treatment as
      the minimum pretreatment requirement for natural wetlands. Natural wet-
      lands, in general,  were perceived to be highly sensitive to cultural use.
      Also, there was great need in Michigan for economical,  post-secondary
      treatment,  including phosphorus removal.  The use of either wetlands or
      upland irrigation for phosphorus removal would be achieved primarily by
      small rural and resort communities in Michigan.  There are only a few
      large communities located near the extensive tracts of undeveloped upland
      or  wetlands needed,  but there are many small communities with wastewater
      flows of  less than 0.5 million gallons per day (MGD) that are so located.
      And a great number of these were already committed to secondary treatment
      by  stabilization ponds.
         We found that wetlands of significant size located within 10 miles
      of  secondary treatment facilities were all bordered by streams and riv-
      ers.   The size of wetlands we had in mind would be such that all of a
      community's wastewater could be applied at less than one inch per week
      during a  6-month season.  Stabilization ponds would store the wastewater
      during the 6-month off-season.  Ten communities were selected to evalu-

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      ate in detail.   The ten had wastewater flows in the range of about 40,000
      gallons per day (GPD) to 350,000 GPD.  All were located near wetlands
      large enough to apply wastewater at the rate of less than 1 inch/week. It
      was assumed that state-owned wetlands would be free, that privately-owned
      wetlands would be $550/acre (updated to 1982), and that a gated irriga-
      tion pipe would be used for applying wastewater to the wetland based on
      the developing design for Hougton Lake.  It was also assumed that chlor-
      ination and dechlorination facilities would be needed and, of course,
      transmission forceline from ponds to wetland would be like any other
      conventional wastewater forceline.  Very few small Michigan communities
      are served with biomechanical secondary treatment systems; however, the
      economic projections should apply to wetland treatment of secondary
      effluent in general.  (AA)
1093.  U.S.  Environmental Protection Agency.  1983a.  Freshwater Wetlands for
         Wastewater Management.  Environmental Impact Statement.  Phase I
         Report - EPA 904/9-83-107.  Region IV, Atlanta.

         The understanding of wetlands values and functions has increased sig-
      nificantly during the past decade. During that period more attention has
      also  been given to the use of wetlands for wastewater management.  With
      increased pressure placed on wetland systems in recent years, regulatory
      and ecological issues have been raised.  This EIS is designed to develop
      tools that can assist local, state and federal agencies in making waste-
      water management decisions affecting wetlands.
1094. U.S. Environmental Protection Agency. 1983b.  The Effects of Wastewater
         Treatment Facilities on Wetlands in the Midwest.  Technical Report.
         Region V 905/3-83-002.  Chicago, Illinois.

         The purpose of this report is to provide background information for
      use in the preparation of a Generic Environmental Impact Statement on
      wastewater management alternatives.  Both natural and constructed (arti-
      ficial) wetlands are considered for this alternative wastewater treat-
      ment technology.  The report describes issues of wastewater and wetlands
      in the Midwest States (Illinois, Indiana, Michigan, Minnesota, Ohio, and
      Wisconsin), summarizes the existing literature on wastewater impacts to
      wetlands (includes a bibliography), inventories the 98 existing wetland
      discharges in EPA Region V, and identifies potential sites and topics for
      additional study.  Key topics for future study include:  constructed
      wetlands, hydrologic impacts, long-term ecological effects, legal and
      administrative constraints, mitigation of impacts, management of receiv-
      ing wetlands, and disease and health considerations.


1095. U.S. Environmental Protection Agency. 1983c.  Wetlands Treatment:  A
         Practical Approach.  EPA Emerging Technology Series.

         This EPA brochure briefly introduces the practical possibilities of
      wetlands treatment of wastewater.  Use of natural versus constructed
      wetlands is differentiated.  Treatment systems design features are sum-
      marized.  A list of currently operating wetland system is presented.
      Potential  limitations of wetland use for wastewater treatment are noted.
      Costs, both capital and O&M are briefly presented.

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1096.  Whigham,  D.F. In press.  Vegetation in Wetlands Receiving Sewage
         Effluent:   The Importance of the Seed Bank.  In  Ecological
         Considerations in Wetlands Treatment of Municipal Wastewaters.
         Proceedings of a  Workshop June 23-25, 1982.  University of Massa-
         chusetts/  Amherst, MA.  U.S.  Fish and Wildlife Service and U.S.
         Environmental Protection Agency.

         Van der Valk (1981, 1982) has proposed a model for studying changes in
      wetland vegetation that is based on three life history attributes:   (1)
      life span, (2) propagule longevity, and (3) propagule establishment
      requirements.  The model assumes that knowledge of these attributes  for
      species in a wetland would enable one to predict vegetation composition
      under various hydrologic (and other environmental) conditions.  Although
      the model needs to be modified or expanded to permit quantification  of
      population properties (e.g., biomass, aerial coverage, etc.), it provides
      a suitable framework to evaluate the types of changes that might occur
      when wetlands are used for wastewater management.
         The purpose of this paper is to discuss the effects that wastewater
      application might have on wetland vegetation. Because two of the three
      attributes used in Van der Valk's model relate to the seed bank, the
      paper will focus primarily on the effects that altered hydrologic and
      nutrient patterns have on recruitment from the seed bank.  (AA)
1097.  Wile,  I.,  G.  Miller and S. Black. In press.  Design and Use of Artificial
         Wetlands.   In  Ecological Considerations in Wetlands Treatment of
         Municipal  Wastewaters.  Proceedings of a Workshop June 23-25, 1982.
         University of Massachusetts, Amherst, MA.  U.S. Fish and Wildlife
         Service  and U.S.  Environmental Protection Agency.

            In recent years, the use of wetlands for wastewater treatment has
      received increasing attention in North America.  To date, attention has
      largely focused on the use of natural wetlands for tertiary treatment.
         Artificial wetlands,  however,  offer greater scope for general use and
      are not restricted by many of the environmental concerns and user
      conflicts  associated with natural wetlands.  Unlike natural wetlands,
      which  are  confined by availability and proximity to the sewage source
      (Sutherland 1981), engineered marshes can be built anywhere, including
      lands  with limited alternative uses.  They also offer greater scope for
      design and management options and thus may provide superior performance
      and reliability.
         Use of  artificial wetlands for year-round treatment of sewage has been
      under  study in Ontario since 1979.  An experimental facility in Listowel,
      Ontario consists of five separate marsh systems (Figure 1) and provides
      flexibility in pretreatment (conventional lagoon;  complete mix aerated
      cell), system configuration, hydraulic loading rates, liquid depths and
      detention  times (Wile, Palmateer and Miller 1981;   Black, Wile and Miller
      1981).  Data  from this facility has been used to develop preliminary
      design and management guidelines.  (AA)

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                                     INDEX

Absorption, 132, 502, 990
Active uptake (nutrients), 817
Adsorption, 132, 241, 259, 330, 950
Aeration, 726, 744
Aerobic, 46, 71, 132, 149, 274, 488, 666, 912
Aerosols, 369, 370, 933
Aesthetics, 1007
Africa, 383
Agricultural wastes, 225, 720, 729, 982
Agriculture, 128, 170, 243, 301, 308, 394, 568, 682, 709, 797, 962
Alaska, 769
Algae, 38, 60, 61, 128, 137, 147, 194, 199, 215, 234, 274, 292, 299, 301, 316,
    339, 353, 364, 393, 429, 433, 436, 449, 458, 485, 498, 504, 519, 520, 528,
    549, 555, 566, 676-678, 685, 697, 733, 756, 757, 759, 761, 762, 763, 770,
    780, 781, 794-797, 834, 912, 913, 917, 954, 955, 963, 968, 993, 994, 1001

Algal growth (bloom), 2, 15, 49, 76, 154, 290, 292, 295, 296, 492, 643, 660,
    727, 757, 856, 857
Alkalinity, 448, 584
Alligator weed, 543, 1023, 1024, 1043
Alluvial, 604
Alum  (flocculation), 797
Aluminum, 509, 986
Ammonia, 48, 76, 154, 284, 292, 295, 316, 332, 436, 446, 448, 451, 567, 623,
    651, 712, 757, 838, 908, 986, 1009, 1019
Ammonium, 95, 408, 631
Amoeba, 586
Amphibians, 403, 1071
Amphipods, 575, 763
Anaerobic processes, 46, 71, 132, 149, 265, 597, 614, 666, 766, 792, 794, 838,
    883, 950, 1019, 1040
Animal Populations, communities, 449, 454, 504, 539, 575, 660, 851, 1071
Animals, 158, 401, 403, 862, 887, 889, 922, 930, 1009, 1071, 1080
Annuals (plants), 993, 998
Anthropogenic, 234, 806
Antibiotics, 316
Aquaculture, 10, 13, 30, 39, 45, 52, 72, 101, 120, 147,  164, 212, 213, 290,
    291, 292, 293, 295, 296, 297, 298, 301, 316, 339, 362, 363, 388, 394, 396,
    398, 464, 465, 468, 471, 476, 491, 500, 503, 548, 555, 567, 573, 591, 594,
    626, 642, 643, 652, 660, 682, 702, 709, 712, 713, 719, 731, 732, 750, 756,
    757, 758, 759, 760, 761, 762, 770, 778, 832, 859, 886, 887, 913, 925, 954,
    961, 962, 968, 983, 1018, 1052, 1057
Aquatic animals (see also animals), 854, 1080
Aquatic communities, 920
Aquatic plants (see also vegetation), 82, 162, 493, 506, 570, 612, 623, 652,
    691, 756, 778, 783, 818, 835, 854, 855, 862, 887, 913, 937, 981, 1016,
    1018, 1020-1022, 1031, 1036, 1040, 1080,  1096
Aquatic weeds, 543, 756, 826, 1096
                                      T-1

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Arbovirus, 167, 168, 368
Arcata, CA, 334
Arctic, 610, 720
Arid, 897
Arizona, 459
Arrow arum, 993
Arrow grass, 516
Arsenic, 194
Arthropods, 403, 576
Artificial Wetlands, 132, 162, 173, 180, 213, 247, 248, 280, 284, 814, 816,
    817, 826, 827-829, 834, 843, 846, 851, 873, 916, 924, 967, 982, 1004, 1005,
    1009, 1013, 1057, 1067, 1069, 1088, 1095, 1097
     1002
Aster(s), 743
Atlantic, 942, 991
Austria, 316, 511
Australia, 709, 720
Avian  (see also birds), 14, 199, 409, 527, 616, 973

Bacteria, 85, 102, 271, 304, 436, 438, 449, 476, 478, 498, 499, 567, 644, 660,
    676, 718, 863, 883, 912, 956, 967, 1061
Bacteriophage, 73, 305, 548, 954
Baitfish, 35, 36, 147
Balaton Lake, 914
Bald cypress (see also cypress), 574
Basin shape, 846
Bass, largemouth, 101, 619
Bays, saltwater, 474, 504
Beaver, 545
Bellaire, MI, 329, 423, 424, 427, 430, 431, 444, 445, 446, 1086
Benthic, benthos, 91, 92, 102, 273, 274, 504, 535, 629, 860, 900
Bermuda grass, 404, 592
Bibliography, 9, 10, 14, 97, 290, 544, 634, 756, 851, 863, 919, 981, 1015, 1057
Bioaccumulation, 513, 629, 851, 1032, 1059
Bioassay, 141, 513, 550, 644, 1059
Biochemical oxygen demand  (BOD), 49, 102, 132, 148, 164, 180, 194, 247, 250,
    251, 284, 364, 404, 584, 843, 863, 925, 956, 1019, 1031, 1036, 1040
Bioconversion, 1028, 1042
Biofilter, 476, 477
Biogas, 567, 756, 1021, 1037
Biogeochemical, 245, 249, 250, 817
Biogenic, 558
Biological clarification, 7, 160, 191, 222, 231, 316, 569, 776, 890, 916, 965,
    967
Biomass, 112, 393, 487, 490, 599, 606, 618, 712, 743, 766, 777, 876, 946, 955,
    991, 994, 995, 996, 1037
Biomagnification  (bioconcentrate), 805
Biophotolysis, 770
                                      1-2

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Birds (see also avian, waterfowl, ducks), 275, 276, 312, 403, 498, 724, 725,
    823, 836, 922, 1071
Bivalves, 152, 215, 388, 549, 759, 761, 942
Black willow  (see also willows), 574
Bladderwort, 857
Bluegill sunfish, 619
Bluegrass, rough stalked, 638
Bog (see also peat, Sphagnum spp.), 108, 161, 317, 318, 454, 482, 584, 640,
    734, 950, 959, 1002
Bog willow, 131
Boston, MA, 234
Botulism, 14, 199, 616, 644, 831
Brackish waters, 497, 498, 499, 500, 554, 744, 950
Britain, 275, 304, 338, 560
Brookhaven National Laboratory, 706, 820, 829
Buffalo, bigmouth, 101
Buffalo, black, 101
Bullheads, yellow, 619
Bulrushes, 17, 27, 173, 174, 247, 483, 516, 788, 843, 846, 967
Bur marigold, 993
Burned, 666

Ca (see calcium)
Cabomba caroliniana (Gray) (see cooter-grass)
Cadmium, 29, 44, 69, 70, 79, 133, 280, 287, 338, 357, 396, 515, 516, 534, 535,
    546, 557, 589, 629, 728, 805, 942, 999, 1000, 1017, 1032
Calcium, 131, 383, 411, 448, 481, 498, 501, 584, 1002
Calico Creek, 22, 768
California, 76, 125, 126, 176, 177, 178, 225, 284, 298, 334, 645, 646, 647,
    648, 649, 720, 748
California grass, 332, 539, 590
Camps, camping areas, 173, 247, 483, 967
Canada, 343, 506, 630, 1004
Canals, 412
Canary grass, 404, 455, 463
Carbohydrate(s), 531
Carbon (total), 921, 1083
Carbon dioxide, 528, 567
Carbon, inorganic, 115, 498, 667, 1079
Carbon, organic, 364, 593, 617, 623, 988, 1036, 1040, 1079
Carex spp. (see sedges)
Carp, 1, 76, 101, 770, 797, 1051
Carp, bighead, 101, 164, 361
Carp, grass, 101
Carp, silver, 101, 164, 361
Case studies, 323, 329
Catfish, channel, 101, 385, 619, 867
Cattail, 66, 67, 76, 89, 250, 251, 383, 404, 531, 578, 581, 817, 829, 876, 908,
    971, 993, 1010, 1040, 1069
                                      1-3

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Cd  (see cadmium)
Ceratophyllum demersum (see coontail)
Ceratophyllum spp.  (see hornwort)
Chamaedaphne calyculata (see leatherleaf)
Channelization, 501
Chelation, 132
Chemical oxygen demand (COD), 137, 194, 247, 250, 251, 284, 448, 584, 1031,
    1040
Chemistry, chemical(s), 49, 132, 390, 431, 433, 436, 445, 461, 515, 582, 634,
    666, 797, 834, 967, 988, 1029, 1037
Chemical treatment, 872
Chesapeake Bay, 58, 138, 256, 357, 373, 554, 919, 960
Chicago, IL, 258
Chinese, China, 101
Chironomid, 199, 273, 589
Chlorella (see algae)
Chloride, chlorine, 181, 194, 438, 447, 501, 584
Chlorinated (hydrocarbons), 541, 554, 948, 950
Chlorination (see also unchlorinated, dechlorinated), 301, 312, 490, 491, 566,
    746, 836, 993, 996, 998, 1010, 1052
Chlorophyll, 232, 256, 273
Chromium, 79, 194, 287, 515, 516, 617, 629
Chromatum vinosum (see bacteria), 956
Chrysanthemum, 690
Cl  (see chlorine)
Clam(s), 396, 762, 763
Clay, 241
Climate, 850, 863
Closed system, 968
Clostridium botulinum (see botulism)
Coagulation, 132
Coal (wastes, ash), 233, 525
Coastal, 97, 468, 515, 521, 526, 544, 613, 641, 805, 806, 837, 850, 889, 921,
    928, 942, 944, 947, 991, 996
Cobalt, 1024
Cold temperatures, 634, 702, 843, 884
Collembolan, 575
Colorado River, 922
Coliform, 16, 102, 247, 250, 251, 270, 284, 316, 449, 478, 666, 718, 883, 950
Columbia, Bogota, 381
Commercial, 504
Community wastewater (see also domestic),  329, 425, 426, 433, 434, 436, 453,
    495, 538, 590, 706, 814, 877, 967, 982, 1007, 1036
Community structure (see also plant and animal community), 154, 773, 1003, 1080
Compost, 568
Conductivity, 284, 448, 584
Conferences (see also symposia), 179, 330, 399, 877, 965, 982, 1011, 1030
Connecticut, 75, 802
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Conservation, 663, 664, 666, 667, 668, 756,  1087
Construction, 163, 1007
Consumer  (communities), 402, 403
Contaminants  (see also pollutants), 514, 950, 960
Cooling tower, 554
Coontail  (Ceratophyllum demersum), 579, 580
Cooter-grass  (Caboiuba caroliniana Gray), 111
Copepods, 273
Copper, 79, 149, 194, 280, 287, 357, 411, 448, 515, 534, 535, 546, 592, 629,
     690,  806, 878
Coprostanol, 560
Cord grass, 90, 119, 130, 234, 253, 280, 287, 324, 325, 336, 513, 515, 516,
     552,  575, 577, 583, 641, 692, 805, 941,  1006
Corkscrew Swamp, Florida, 211
Costs, capitol, 670, 671, 756, 872, 886, 910, 983, 1010, 1095
Cr  (see chromium)
Crabs, 490, 491, 660
Crappies, black, 619
Crops, 554, 651, 690, 766, 770, 823, 838, 839, 931
Crustaceans, 57, 151, 227, 274, 307, 498, 757-766, 770, 963
Cu  (see copper)
Cucumber, 411, 690
Cut  grass, 993
Cynadon spp.  (see Bermuda grass)
Cyperus spp., 606
Cypress dome, 16, 73, 74, 80, 91, 186, 188, 208, 235, 236, 237, 241, 262, 263,
     266,  306, 309, 350, 351, 352, 401, 402, 403, 576, 606, 624, 661, 716, 718,
     724,  725, 817, 865, 974
Cypress pond, 28, 109, 669, 866
Cypress strand, 239, 270, 672
Cypress swamp, 168, 171, 211, 222, 238, 240, 262, 263, 264, 265, 266, 267, 268,
     475,  598, 599, 600, 604, 607, 632, 633, 662-673, 717, 773, 833, 950, 965,
Czechoslovakia, 219

Daphnia,  189, 190, 272, 273, 353, 578
Data collection (methods), 1050, 1075, 1076
Deactivation, 921
Dechlorination, 746, 848
Decomposition, 93, 131, 171, 208, 277, 300, 740, 741, 743, 803, 807, 811, 948,
    950. 951, 998
Deep water,  790
Deer, 207
Dehydrogenase, 883
Delaware,  181, 993, 998
Delaware River, 803, 808, 993,  994, 996
Denitrification (see also nitrogen), 48,  130, 186, 265, 307, 308, 333, 476,
    726, 743,  792, 817, 826, 866, 921
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Desert, marsh system, 459, 922
Design, 671, 706, 726, 739, 826, 827, 863, 886, 887, 910, 929, 961,  1004, 1007,
    1010, 1018, 1057, 1075, 1076, 1088, 1095, 1097
Detergents (soaps), 1, 359, 750, 755
Detoxification, 328, 524, 746
Detrital, 76, 1079
Detritus, 994, 1051, 1079
Developing countries, 623
Development, 658
Diatoms, 72, 396, 758, 759, 761, 953
Discharges,  treatment plant (see also effluent), 920
Disney World, 496
Dissolved organic matter, 316
Dissolved oxygen (DO), 5, 49, 76, 102, 180, 528, 535, 744
Distichlis spicata (see spike grass)
Disturbed land, 1050
Diurnal, 426, 438, 656, 1032
Domestic waste, 472, 492, 567, 568, 634, 643, 705, 706, 759, 760, 761, 817,
    858, 912, 957
Drainage, 158
Dredge, dredged material, 210, 257, 466, 513-515, 541, 550, 587, 805, 806, 900,
    1014
Drinking Water, 709, 820, 821, 823, 825
Drummona, WI, 21, 318, 453, 454, 1002
Ducks (see also waterfowl), 126, 199, 354, 451, 453, 749, 999, 1000
Duckweed, 159, 237, 241, 265, 345, 374, 528, 535, 666, 712, 717, 766, 1031

East River,  New York City, 750
Ecology, ecological effects of wastewater (general), 62, 184, 319, 970, 992
    1074, 1080, 1089, 1093
Economics, 142, 240, 262, 363, 605, 664, 666, 832, 875, 925, 1007, 1036, 1048,
    1057, 1061, 1075, 1076, 1092
Ecosystem (functioning, dynamics), 115, 139, 229, 238, 416-420, 439-443, 472,
    499, 500, 604, 656, 659, 660, 686-688, 727, 846, 882, 998, 1047, 1074, 1082
Ecosystem diversity, 75, 236
Education, 180. 639, 640, 922
Eels, 770
Effluent (see also tertiary and secondary effluent), 416-420, 439-443, 455,
Effl538, 617, 618, 623, 674, 695, 750, 764, 783, 797, 813, 846, 922, 966, 974,
    975, 980, 983, 996, 998, 1047, 1052-1055, 1061
Eh  280, 535
Elements (budgets), 809, 818, 909
Elodea canadensis, 557
Elemgent vegetation (see also macrophytes, plants, vegetation), 162, 373, 479-
    481, 532, 618, 698, 843, 845, 846, 882
Encephalitis, 240
Energetics,  666
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Energy, 624, 661, 714, 766, 770, 827, 843, 885, 886, 969, 1010,  1021,  1028
Engineering, 52, 337, 652, 731, 732, 778, 827, 872, 873, 884, 886,  1010, 1018
     1088
England (See Britain)
Enteric pathogens (see also human enteroviruses), 759
Enteromorpha spp.   (see algae)
Environmental impact statement, 77, 615, 1093
Environmental Protection Agency (U.S.), 50, 51, 924, 925, 928, 929, 930, 1070,
     1094
Epibenthic algae (see also algae), 993, 998
Epidemiology, 609
Epiphytes, 593
Escherichia coli, 46
Estuary, estuarine, 77, 97, 102, 103, 129, 134, 148, 153, 154, 156, 181, 234,
     256, 304, 311,  357, 376, 378, 399, 473, 498-500, 504, 521, 534, 544, 554,
     560, 659, 660,  682, 746, 772, 806, 834, 837, 848, 900, 928,  947, 950, 960,
     963, 1006
Euglena gracilis (see algae)
Europe, 79, 494, 817
Eutrophic, 34, 392, 399, 511, 519, 529, 641, 643, 752, 765, 797, 834,  914, 917,
     1056, 1082
Evaporation, 826
Evapotranspiration, 420, 664, 843, 1037
Everglades marsh, 140, 169, 856, 857

Facultative (ponds), 1009, 1031, 1036
Farm, farming, 489, 554, 567, 597
Fatty acids, 956
Fe  (see iron)
Feasibility (study), 874, 909, 910, 921, 1007, 1054
Fecal coliform (see also coliform), 46, 102, 194, 245, 284, 716
Fecal pollutant, 560
Fecal streptococcus (see also streptococcus), 102, 284
Feed (see also food), 40, 159, 983
Fertilizer, 108, 232, 287, 561, 612, 756, 770, 913, 948, 953, 1021, 1037
Fescue (see also grass), 149
Fiber, 40, 404, 777
Fiddler crab (see also crab), 234, 287
Films (system), 625, 739, 1019, 1040
Filter, soil, 242, 638, 841, 927
Filter, vegetative, 6, 514, 888, 958
Filtration, 96, 132, 986, 1066
Finland, 452, 507, 804, 817, 871
Fish, 1, 5, 8, 57, 77, 99, 121, 147, 150, 212, 213, 301, 327, 334,  359-362,
    375, 386, 394, 494, 497, 498, 525, 542, 543, 582, 642, 643,  681, 712, 733,
    770, 774, 778, 797, 823, 847, 848, 867, 913, 918, 919, 963,  983
Fish culture,  farming, 9, 10, 35, 135, 142, 164, 204, 468, 567,  568, 573, 642,
    681
Flagellates (intestinal), 586
Floating aquatic vascular plants, 618
                                      1-7

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Flocculation, 697
Flood, 55, 70, 227, 467, 502, 513, 891, 922
Flood basin, 29
Flood conditions, 640, 729, 805, 891
Flood plain, 405, 640, 667, 745
Flora (see plants, vegetation)
Florida, 53, 75, 84, 85, 143, 168, 171, 186, 201, 208, 209, 236, 239-241, 264,
    269-271, 306, 307, 309, 350, 351, 367, 403, 475, 519, 576, 598-601,
    606-608, 632, 661-672, 717, 724, 725, 772, 817, 826, 833, 858, 866, 900,
    969, 987, 1061, 1063, 1064
Flounder(s), 759, 761, 763
Flow (wastewater, stream), 270, 284, 431, 438, 445, 521, 726, 757, 758
Flushing, 698
Flies, 823
Fodder, 770
Foliage, 1002
Food (see also feed), 190, 387, 404, 713, 765, 777, 1021, 1037, 1057
Food chain (or web), 212, 294, 301, 396, 549, 554, 555, 643, 713, 757-760,
    762-764, 778, 863, 867, 930, 968, 1006
Forage, 147, 775
Foraminifera, 498, 523
Forests, forestry, 95, 220, 243, 445, 452, 574, 603, 640, 649, 666, 689, 695,
    839, 892, 894, 895, 987
Freshwater, 45, 300, 367, 376, 415, 426, 447, 479-481, 497, 520, 766, 803, 805,
    807, 808, 810, 811, 817, 834, 906, 908, 910, 921, 950, 951, 963, 972, 989,
    990, 993-998, 1013, 1037, 1063, 1064
Fungus, fungal, 145, 146, 208, 555, 697

Gambusia affinis (see mosquitofish)
Gamma radiation, 867
Gasoline (see petroleum)
Geology, geologic, 241, 666, 667
Georgia, 130, 376, 574, 864, 901, 986
Geochemistry (see also chemistry), 613
Geothermal, 88, 851
Germany, 495, 817
Giardia lamblia (Giardiasis), 258
Glacial, 973
Glyceria grandis (see reed-meadow grass or manna grass)
Grass (grassland), 149, 287, 638, 695, 743, 876
Great Lakes, 513, 617, 805
Great Sippewissett Marsh, MA, 287, 583
Greywater, 756
Greenhouse(s), 280, 1037
Groundwater, 186, 245, 564, 792, 820, 821, 824, 841, 910, 926, 949, 962, 978
Growth (animal), 867, 1051
Growth (plant), 69, 109, 531, 865, 948, 980, 995
Gulf coast, 861
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Habitat, 32, 68, 75, 77, 103, 128, 129, 176, 180, 221, 275, 312, 409, 525, 527
    541, 542, 834, 876, 911, 922, 950, 1071
Hallbeard tearthumb, 993
Hardwood swamp, 84, 606, 1061
Harvest, 248, 299, 502, 630, 643, 697, 817, 826, 843, 846, 861, 876, 913, 1056
Hawaii, 203, 332
Hazardous wastes (see also toxins, pathogens), 1040, 1059
Health, 486, 609, 821, 886, 913, 932-934, 1048, 1049, 1071, 1091
Heat (see also thermal pollution), 154, 643, 842, 900, 1057
Heavy metals (see also trace elements), 29, 68, 79, 122, 123, 149, 213, 217,
    236, 255, 257, 265, 270, 279, 284, 287, 304, 311, 348, 374, 438, 464, 465,
    466, 475, 513, 515, 516, 541, 546, 548, 554, 570, 579, 581, 614, 617, 618,
    620, 629, 666, 667, 690, 767, 790, 805, 806, 809, 863, 900, 935, 942, 948,
    950, 984, 1001, 1014, 1021, 1032,  1089
Helminths, 255, 586, 935
Herbivory, herbivorous, 494
Heterothrophy, 304
Hg (see mercury)
Histopathologic, 1000
Histosols, 246
Holland (see Netherlands)
Hornwort, 535
Hot climates, 756
Houghton Lake, MI, 26, 100, 329, 406, 414, 428, 429, 433, 434, 436, 437, 446,
    449, 545, 700, 722, 875, 980, 1007, 1008, 1009, 1010, 1015, 1083, 1084
Hudson River, 103, 806
Human enteroviruses (see also viruses),* 305, 438, 548, 591
Humic acid, 509
Hungary, 914
Hunting, 922
Hydrilla spp., 766
Hydrocarbons, 152, 213, 490, 491
Hydrogen peroxide, 848
Hydrogen sulfide (K^S), 119
Hydrogeology, 322, 683, 833
Hydrology (hydraulics), 245, 284, 341, 350, 351, 420-422, 429, 433, 438, 449,
    499, 639, 817, 850, 910, 951, 1080, 1087, 1089
Hydroperiod, 54
Hydroponic, 404, 411,  516, 690

Ice (cover), 49
Ictalurus natalis (see bullhead, yellow)
Ictalurus punctatus (see catfish, channel)
Illinois,  603-605
India,  492, 592
Indiana, 393
Indicator species, 610
Industrial wastes, 77, 142, 274, 304, 376, 399, 592, 595, 682, 834, 956, 957,
    1030
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Inorganic carbon (see carbon, inorganic)
Inorganic nitrogen, 729
Insecticide (see also mevinphos), 1016
Insects, 227, 469, 498, 589, 695, 1015
In-situ, 986
Invertebrates, 92, 102, 104, 109, 110, 199, 212, 213, 429, 436, 451, 497, 616,
    629, 753, 763, 778, 913, 1071
Iowa, 484, 971
Iron, 194, 287, 357, 411, 502,  509, 516, 629, 690, 805, 986, 1006
Irradiation, 555
Irrigation, 3, 31, 32, 41, 68,  115, 128, 312, 329, 332, 368, 394, 419, 421,
    422, 436, 446, 655, 737, 756, 797, 803, 807, 811, 836, 840, 869, 899, 905,
    962, 977, 989, 993, 997,^ 1007, 1009, 1048, 1058, 1060, 1061
Israel, 797

Joint grass, 1016

K (see potassium)
Kidneys, 1000
Kincheloe/Kinross Air Force Base, 67, 309
Kinetics, 726, 758
Kissimee River Valley, 75, 407, 703

Labrador tea, 1002
Lacustrine, 817
Lagoons, 35, 46, 49, 137, 199,  227, 273, 274, 286, 303, 318, 375, 424, 463,
    469, 478, 489, 529, 565, 597, 626, 628, 630, 634, 655, 720, 726, 739, 770,
    775, 777, 925, 956, 957, 961, 1025, 1031, 1036
Lake Meade, 459
Lake Okeechobee, 826
Lakes, 114, 115, 288, 511, 537, 570, 588, 599, 601, 680, 705, 715, 910, 914
Land, 114
Land application, 470, 738, 885, 921, 929, 961
Land disposal, 3
Landscape, 661, 664
Land treatment, 81
Land use, 910, 962
Larvae, 497, 583
Leaching, 383, 651
Lead, 44, 194, 210, 280, 287, 338, 357, 448, 515, 516, 534, 546, 557, 589, 617,
    629, 690, 728, 802, 806, 942, 1023
Leatherleaf, 131
Leaves (coniferous, deciduous), 241
Legal, 536, 706, 745, 910, 962, 1090
Legislation, 750
Lemna spp. (see duckweed)
Lepomis macrochirus (see bluegill sunfish)
Lettuce, 411, 690
Light (see also solar), 535, 712, 1032
Lime (see also quicklime), 316
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Limnology, 273, 274
Literature review, 759
Litter, litterfall, 131, 171, 208, 236, 433, 663, 743, 951, 996, 998
Littoral, 219, 593, 680, 705, 715
Livestock waste, 46
Lobsters, 759, 761, 763
Long-term effects, 67, 1072
Louisiana, 227, 302, 692, 698, 817, 834, 850, 921, 988

McNaughton Marsh, WI, 533
Macroinvertebrates (see also invertebrates), 91, 451, 493, 535
Macrophytes  (see also plants, vegetation), 570, 588, 631, 653, 697, 699, 723,
    766, 786, 787, 791, 812, 818, 869
Magnesium, 131, 194, 383, 448, 481, 501, 986
Maine, 489
Maintenance, 1012, 1095
Mammals, 31, 68, 403, 695, 1071
Management,  300, 529, 531, 603, 625, 648, 663, 664, 666-668, 759, 782, 806,
    814, 817, 853, 877, 886, 887, 940, 961, 971-973, 981, 987, 994, 1083,
    1084, 1089, 1090, 1093, 1097
Manganese, 194, 210, 287, 357, 411, 629, 690, 805, 986, 1006
Mangrove, 365, 789, 938, 950
Market, 747
Marine, 22,  23, 45, 57, 72, 215, 227, 291-293, 295, 296, 387, 388, 399, 464,
    465, 468, 521, 546, 643, 659, 764, 765, 757-763, 770, 860, 1059
Marsh(es) (natural), 29, 64, 76, 79, 106, 132, 201, 220, 227, 248-251, 281,
    284, 315, 341, 367, 376, 412, 479-481, 489, 515-518, 532, 541, 544, 556,
    562, 563, 575, 617, 618, 640, 645-649, 660, 698, 706, 715, 798, 805, 814,
    820-825, 826, 828, 834, 837, 843, 846, 850, 856, 861, 922, 950, 961, 971,
    972, 981, 994, 1048, 1063, 1064, 1068
Marsilea quadrifolia (see water clover)
Maryland, 58, 357, 554, 919, 960
Massachusetts, 106, 232, 234, 245, 287, 538, 555, 583, 1052, 1053, 1054, 1055,
    1057
Mathematical models, 198
Meadow(s), 820, 821, 825, 826, 829
M-cresol, 1040
Meiofauna, 498
Mercury, 71, 194, 280, 328, 354, 507, 515, 592, 1023
Mass balance, 990
Metabolism, 667
Metals (see also heavy metals, trace elements), 541, 546, 618, 902, 942
Methane, 567, 1042
Mevinphos, 1016,  1022
Mg (see magnesium)
Michigan, 66, 67, 115, 131, 230, 273, 274, 315, 322, 414, 416-425, 427-430,
    433, 434, 436, 437, 440-446, 449, 545, 564, 589, 596, 624, 700, 722, 741,
    743, 753, 779-781, 817, 872, 873, 904, 906, 908, 918, 959, 980-982, 1007,
    1009, 1010, 1015,  1092
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Michigan State University, 42
Microbes, 663, 666, 667, 1073, 1079, 1091
Microbiology, 726, 1073, 1079, 1091
Microbial action, 132, 376, 399, 817, 883, 956, 957, 1073, 1079, 1091
Microceptis aeruginosa (see algae)
Micro-organism, 173, 666, 912, 950, 1073, 1079, 1091
Micropterus salmoides (see bass, largemouth)
Middle East, 142
Midges, 227, 573
Midwest, 227, 469, 1070, 1094
Migratory birds (see also birds, waterfowl), 349
Mineral, 479, 480, 533, 554, 1037
Mines (mine drainage), 6
Minnesota, 243, 318, 635-637, 714, 852, 1066
Minnows, 35, 375, 484, 596, 918, 963
Mississippi, 850, 1017-1019, 1021-1032, 1036, 1037, 1040-1043, 1072
Missouri, 720
Mites, 227
Mn (see manganese)
Models (see also mathematical models), 198, 223, 245, 271, 378, 399, 521, 542,
    546, 564, 599, 601, 651, 661, 667, 686-688, 715, 741, 760, 789, 826, 848,
    954, 960, 1075, 1076, 1077, 1078, 1086, 1096
Molluscs (see also clams, oysters), 215, 388, 396, 498, 548, 719, 758, 759,
    761-763, 770
Mortality, 995
Moss (see Sphagnum spp., peat)
Mosquito, 167, 227, 340, 368, 609, 720, 823, 1058
Mosquitofish, 76, 168, 648
Mouse, white-footed, 695
Muck, 561, 883, 1044
Multiple use, 102
Municipal wastewater (sewage), 77, 201, 207, 212, 213, 225, 230, 274, 390, 448,
    506, 535, 544, 552, 594, 596, 613, 624, 641, 660, 680-682, 707, 747, 775,
    778, 791, 834, 837, 839, 840, 845, 872, 876, 904, 918, 928, 929, 955, 962,
    1013, 1052, 1053, 1061, 1063, 1064, 1068, 1073
Muskellunge (muskies), 375, 596, 918
Muskrat, 585
Mussels, 148, 234, 287, 546, 549, 763
Mycorrhizae, 553

N (see nitrogen)
Na (see sodium)
Napier grass, 404
National Science Foundation, 100
National wildlife refuge(s), 341, 707, 1052
Natural waters (wetlands) (see also swamps, marshes, bogs, peat, salt
    marshes), 213, 890, 1004, 1076, 1086, 1095
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Navicula salinarum  (see diatoms)
Needles (cypress, pine), 171, 236, 265
Nematode, 255
Netherlands, 173, 412, 644, 817
Nevada, 922
New England, 287
New Jersey, 311, 556, 695, 817, 992, 993, 996, 998
New Orleans, LA, 850
New York, 103, 162, 349, 750
New Zealand, 346, 347
Ni (see nickel)
Nickel, 79, 194, 448, 515, 516, 629, 690, 1017
Nitrate, 48, 95, 102, 149, 226, 227, 247, 250, 284, 295, 332, 339, 377, 411,
    436, 446, 448, 502, 510, 631, 651, 690, 693, 694, 712, 757, 908, 986, 1009
Nitrification, 336, 408, 726, 817, 866
Nitrite, 284, 332, 444, 448, 908
Nitrogen, 3, 48, 75, 95, 115, 130-132, 173, 194, 214, 222, 240, 245, 265, 270,
    271, 284, 291, 292, 295, 296, 308, 332, 353, 367, 377, 383, 415, 431, 438,
    445, 446, 456, 470, 476, 498, 501, 510, 511, 556, 566, 584, 589, 617, 637,
    651, 660, 667, 692, 698, 700, 729, 743, 750, 759, 760, 761, 790, 800, 817,
    838, 857, 863, 889, 906, 908, 921, 922, 931, 944, 950-952, 965, 968, 986,
    990, 996, 998, 1002, 1009, 1019, 1021, 1031, 1052, 1077, 1078, 1086
Nitrogen cycle, 95, 460, 640
Nitrogenous gases, 48
N2 (see nitrogen)
NO2 (see nitrite)
NO3 (nitrate)
Nocturnal, 76
Nonflood conditions (see also flood), 70
Nonpoint source pollution (see also pollutants), 284, 682, 826
North Carolina, 153, 154, 323, 498-500, 660
North Dakota, 956, 957
Northwest Territories, Canada, 343, 391
Nutrient film technique, 404
Nutrients, 53, 77, 119, 131, 180, 213, 236, 270, 292, 300, 301, 309, 367, 383,
    404, 414, 436, 438, 440, 446, 458, 466, 497, 499, 504, 532, 563, 593, 599,
    618, 643, 663, 664, 666, 667, 669, 690, 698, 741, 742, 743, 758-764, 766,
    769, 781, 817, 826, 834, 843, 857, 858, 865, 876, 889, 903, 905, 910, 913,
    921, 925, 943, 946, 947, 949, 959, 980, 989, 990, 993, 994, 996, 997, 1002,
    1009,  1036, 1037, 1047, 1072
Nutrient(s) cycling, 115, 188, 245, 301, 460, 479, 480, 481, 518, 519, 773,
    843, 1077, 1078, 1089
Nutrient(s) movement, 93, 704, 715, 810, 843, 906, 949, 959, 990, 994, 1077,
    1078
Nutrient(s) removal, 53, 58, 82, 84, 85, 94, 155, 222, 240, 241, 345, 429, 448,
    636, 637, 751, 766, 793, 812, 835, 855, 890, 908, 910, 965, 1014, 1052,
    1053,  1061, 1063, 1064,  1066, 1086, 1089
Nutrient(s) sink, 473, 743,  986, 993, 994
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02 (see oxygen)
Ocean disposal, 1059
Odor, 823
Ohio, 629, 695
Oil refinery effluent (see also petroleum, hydrocarbons), 206
Oligotrophic, 34
Okefenokee Cypress Swamp Forest, 773
Oklahoma, 147, 206
Ombrotrophy, 317
Ondatra zibethica (see muskrat)
Ontario, Canada, 344, 617, 618, 630, 790, 791, 814, 1004, 1069, 1097
Oregon, 278
Organic(s), 213, 548, 558, 563, 566, 623, 863
Organic carbon (see carbon)
Organic chemical waste (see also toxins), 926, 1040
Organic matter, 632, 633, 726, 994, 1057
Organic soil (see also histosol), 242, 244, 259, 509, 635, 990, 979
Oryza sativa (see rice)
Overland flow (see also flow), 435, 463
Oxidation, 227, 284, 334, 353, 364, 466, 497, 500, 589, 616, 720, 726, 813,
    867, 883, 912, 918
Oxidation ponds, 286, 678, 868
Oxygen, 226, 393, 399, 563, 589, 640
Oxygen demand (see also BOD), 245
Oysters, 181, 295, 296, 396, 548, 549, 746, 757, 759, 761-763, 815, 968

P (see phosphorus)
Palustrine, 817
Panicum purpurascans (see California grass)
Papyrus, 283, 950
Particulates, 949
Pathogens (see also bacteria, viruses), 85, 132, 144, 213, 224, 227, 429, 492,
    586, 759, 899, 930, 1000, 1060, 1080, 1091
Patuxent River Estuary (see also Chesapeake Bay), 256
Peat (land, moss, resources) (see also moss, Sphagnum spp.), 96, 133, 157, 161,
    243, 244, 330, 414, 420, 421, 429, 433, 436, 440, 449, 452, 507, 545, 561,
    584, 624, 635-637, 689, 734, 740-743, 804, 817, 852, 870, 871, 903, 959,
    980, 990, 1002, 1007, 1009, 1010, 1011, 1041, 1066
Pennine moorland (see also bog), 158
Pennsylvania, 310, 312, 562, 563, 706
Peppers, 411
Perennials, 993
Periphyton, 857
Peromyscus leucopus (see mouse, white-footed)
Pesticides, 438, 524, 622
Petroleum (see also gasoline, hydrocarbons, oil), 77, 595, 725, 950, 988
pH, 76, 280, 284, 448, 466, 499, 513, 535, 584, 712, 728, 743, 838
Phalaris spp. (see canary grass)
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 Phenol,  788,  1040
 Phosphates (ortho),  34,  102,  247,  250,  256,  284,  313,  330,  344,  375,  411,  436,
     510,  583,  623,  690,  757,  797,  864,  901,  986
 Phosphorus,  3  75, 95,  115,  131,  132,  169,  171,  173,  194,  200,  202,  214,  234,
     240,  241,  245,  247,  248,  250,  251,  259,  265,  270,  271,  295,  335,  343,  353,
     367,  383,  396,  397,  407,  415,  431,  438,  440,  445,  446,  448,  455,  456,  460,
     470,  481,  487,  497-499, 501, 502, 509,  511, 516,  519,  579,  580,  584, 589,
     604,  617,  632,  633,  637,  660,  667,  674,  675,  692,  698,  700,  701,  743,  750,
     800,  816,  8x7,  826,  843,  846,  857,  863,  876,  879,  908,  915,  921,  922,  950,
     951,  965,  968,  986,  990,  996,  998,  1003,  1010,  1019,  1021,  1031,  1044,  1052
     1066,  1077,  1078,  1086
 Photographic pollutants,  1029
 Photosynthesis,  photosynthetic,  49, 76,  225,  660, 679
 Phragmites conununis  (see  reed, common)
 Physical  alteration  (see  also construction),  77,  634,  960
 Phytoplankton, 102,  154,  215, 216, 274,  284,  295, 316,  388,  498,  499,  548,  582,
     599,  628,  675, 750,  758,  762,  764,  768,  770
 Phytotoxicity  (see also  toxins), 513
 Pickerelweed,  29
 Piedmont  Swamp,  380, 986
 Pimephales promelas  (see  also minnows,  fathead),  35
 Pine(s),  606,  669, 724
 Plant(s)  (see  also vegetation), 69, 79,  87,  149,  166,  236,  278,  280,  284,  338,
     404,  440,  449, 466, 479-481, 497, 504, 506, 513, 515, 516, 532, 539-541,
     557,  558,  570, 575, 588,  592-594, 743, 767, 779, 784, 803, 838, 1080
 Plant disease(s), 230
 Plant tissue,  149
 Plankton  (planktonic algae) (see also phytoplankton, zooplankton), 299,  346,
     347,  396,  504
 Poa  trivialis L. (see bluegrass)
 Poland, 705, 882, 1044
 Polio virus, 72, 73, 457, 548, 869
 Political, 536
 Pollution, pollutants, 132, 284, 504, 623, 639, 640, 643, 757, 758, 770, 772,
     843, 846. 889, 916, 922,  1021, 1037, 1041
 Polychaete worms, 763, 1065
 Polyculture (see also food chain), 757-764, 766, 778, 867
 Pomixis nigromaculatus (see crappie, black)
 Ponds, 8, 49, 86, 174, 189, 225, 231, 284, 289, 303, 334, 353, 364, 470, 500,
     528, 535, 572, 589, 616, 634, 660, 685, 706, 733, 744, 797, 820-826, 828,
     829, 849, 867, 912, 918, 994, 1009,   1048, 1057
Pondweed, 535
Population(s)  (dynamics)  (see also fish, animal, plant, algae, etc), 68, 713,
     1007
Portugal, 682
Potassium, 131, 194, 383, 411, 448, 481, 501, 675
Potomac River,  156,  185, 848,  920
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Potomogeton spp. (see pondweed)
Power plant effluent, 295
Power plant emissions, 900
Prawns (Malaysian), 476
Precipitation, atmospheric, 158, 420, 949
Precipitation, chemical, 817
Predator-prey, 227
Primary effluent (see also sewage, raw), 843, 846, 1036
Primary production, 256, 273, 284, 300, 533, 727, 741, 766, 861, 950, 989, 991,
    994, 997, 998, 1080
Primary treatment, 102, 271, 364, 859
Productivity, 112, 253, 532, 533, 639, 640, 667, 677, 698, 740, 768, 769, 816,
    908, 921, 946, 948, 980, 1080, 1089
Protein, 750, 770, 797
Protozoa, 274, 504
Puerto Rico, 544
Purification, 784, 785, 787, 804, 846, 916

Quality, water, 64, 307
Quicklime, 316

Rabbit(s), 68
Raceway, 148, 925
Radioactive isotopes, 771
Recharging (water table) (see also groundwater, recharge), 640, 663, 666, 667,
    709
Reclamation, 111, 125, 128, 212, 220, 221, 225, 284, 294, 411, 566, 578, 591,
    610, 830, 1081
Recreation, recreational, 103, 180, 284, 504, 590, 639, 640, 682, 689, 922, 967
Recycling (see also nutrient cycling), 114, 168, 238, 240, 266, 293, 468, 663,
    664, 666-668, 670, 671, 710, 765, 757-764, 766, 794, 962, 1048, 1067
Redox potential, 226, 280, 466, 513, 728
Reed, common, 173, 174, 217, 511, 914, 1019, 1040
Reed-meadow grass, 617
Refuges (see National Wildlife Refuge)
Regulation(s), 1007,  1093
Rehabilitation, wetland, 282
Reptiles, 403, 1073
Research (projects),  835, 922
Reservoirs, 667, 729
Resource(s), 885, 886, 1067
Respiration, 48, 154, 660
Restocking, 147
Restoration, 143, 180, 1081
Reuse (water), 756, 830, 876
Rhizome(s), 284, 516
Rhode Island, 641
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Rice, wild, 70, 280, 728, 770, 995
Riparian (see also riverine), 405, 608, 640 891-897, 922, 940, 964
River, 223, 592, 901, 960, 985, 1032, 1072
Riverine, 95, 343, 481, 817, 848, 872, 985
Rodent, 823
Rooted aquatic macrophyte (see also plants, emergent vegetation,  submerged
    vegetation), 557, 711, 818, 845, 937
Roots, 236, 241, 265, 284, 344, 516, 651, 908, 1032
Rotenone, 616
Rotifers, 274
Runoff, 186, 372, 701, 703, 951
Rural communities, 961, 1007
Rush, 1016

Safety factors, 1059
Salinity, 102, 280, 476, 629, 922
Salmo spp.  (see trout)
Salmon, 11-13
Salmonella, 46, 252
Salt marsh(es), 44, 56, 79, 119, 130, 232, 253, 287, 325, 498, 534, 583, 641,
    690, 721, 802, 888, 942-944, 946-950, 953, 1003, 1014
Saltwater (sea), 11, 96, 548, 639, 643, 660, 757-763, 805, 954
Sand, sandy soil, 73, 74, 937
San Francisco Bay, 76, 180
Sanitation, 756
Sawgrass, 817, 857
Scallops, 547, 763
Sea grasses, 900
Season(s),  seasonal, 49, 76, 236, 237, 438, 810, 829, 843, 846, 948, 951, 1009,
    1052
Seawater (see saltwater)
Seaweed, 295, 759, 761-763
Secondary effluent, 179, 180, 186, 263, 264, 269, 271, 333, 433, 448, 459,
    463-465, 468, 482, 500, 548, 660, 690, 707, 741, 750, 753, 757, 759-763,
    779, 843, 846, 850, 852, 866, 904, 908, 966, 996, 998, 1010, 1052
Secondary treatment, 48, 115, 271, 284, 301, 429, 445, 459, 859
Sedge(s), 34, 131, 316, 344, 513, 516, 743, 805, 908
Sediment, 132, 287, 306, 309, 338, 344, 357, 400, 412, 460, 466, 487, 513, 535,
    554, 570, 592, 617, 618, 629, 640, 667, 694, 790, 805, 806, 850, 856, 857,
    866, 889, 942, 950, 988, 1002
Sedimentation, 866
Scenedesmus acutus (see algae)
Semi-arid, 586
Septic tank systems, 247, 696
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Sewage, raw, 203, 271, 529, 628, 720, 829, 838, 848
Shellfish, 253, 349, 628, 643, 757-763, 968
Shrimp, 744, 963
Shrub (growth), 667
Siltation, 640
Silver, 1024
Silviculture, 962
Simian rotarirus, 305
Simulated sewage effluent, 980
Site (selection), 671, 683, 706
Skokie Marsh Area, 43
Sludge (digestion, disposal), 555, 614, 651, 726, 739, 759, 806, 820, 838, 839,
    869, 929, 936, 942, 943, 947, 948, 950, 953, 1051, 1057, 1067
Small-scale facilities, 709
Sminthurides rubronivius (see collembolan), 575
Snails, 234, 552
Soap(s) (see also detergents), 359
Social, 536
Socioeconomic, 75
Sodium, 131, 149, 383, 411, 448, 501, 986
Sodium thiosulfate, 746
Soil, 3, 87, 149, 305, 348, 377, 400, 429, 433, 436, 449, 461, 520, 540, 541,
    554, 561, 651, 669, 683, 698, 743, 759, 779, 838, 876, 891, 904, 908, 921,
    930, 977, 980, 994
Soil profiles, 502
Solar energy, 298, 404, 497, 499, 859, 1028
Soiids, 544, 739, 863
Sorghum, 838
Sorption, 817, 921
South Dakota, 655
Southeast USA, 473
South Carolina, 782
Southwest USA, 363, 386, 720, 925
Sowbugs, 227
Spartina spp. (see cord grass)
Species composition, 953, 993
Sphagnum spp. (see also peat), 454, 734, 804
Spiders, 227
Spike grass, 280, 513, 515, 516
Spirodela polyrhiza (see water-flaxseed)
Spray irrigation, 842, 989
Spruce, 454
Spruce, black, 1002
St. Croix, Virgin Islands, 111
Stabilization ponds (see also ponds), 189, 194, 231, 245, 284, 364, 424, 469,
    485, 492, 535, 586, 594, 596, 634, 720, 727, 739, 797, 872, 918
Standing crop, 344, 497, 498, 921, 948, 996
Storm water, 29, 132, 371, 399, 922, 979, 1066
Strands, 667
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Stratification, 849
Stream(s), 5 117, 233, 343, 392, 519, 588, 634, 640, 910
Stress, 185, 203, 920, 950, 1079
Strontium, 1024
Submerged vascular plants, 535, 570, 578, 593, 617, 680
Subsurface, 3
Succession, 585, 593
Suisun Marsh, Sacramento CA, 125-128
Sulfur, 513
Surface water, 186, 433, 445, 452, 908, 909, 962, 994
Suspended particulates, 78, 132, 164, 180, 194, 213, 250, 343,  353,  404, 438,
    584, 601, 922, 1019, 1036
Swamp(s), 34, 132, 233, 343, 344, 376, 383, 985
Sweet flag, 993
Swine, 101, 149, 182,  183, 982
Switzerland, 1049
Symposia, 18-20, 52, 128, 404, 910, 920, 921, 990,  1015

Tabanid, 583
Talorchestia longicornis (see amphipod), 575
Taxodium distichum (see bald cypress)
Taylor Creek - Nubbin Slough Basin, 407, 703
Temperate climates, 759, 761
Temperature, 49, 102,  153, 154, 284, 497, 521, 535, 936, 1032
Terrestrial (see also uplands), 1047, 1048
Tertiary treatment, 21, 25, 59, 84, 86, 115, 131, 215, 240, 241, 262, 263, 265-
    271, 275, 291, 295, 296, 319, 327, 352, 385, 386, 388, 414, 426, 429, 445,
    447, 449, 484, 497, 498, 506, 549, 584, 634, 641, 663, 664, 666-668, 708,
    727, 757, 758, 764, 859, 872, 903, 908, 923, 954, 968, 994, 1007, 1009-
    1011, 1061
Testes, 1000
Texas, 189, 195, 288, 629, 685, 898
Textile waste, 963
Thalassia testudinum (see sea grass)
Thermal pollution, 399, 468, 574, 643, 848, 851, 867, 900, 941
Thermodynamic, 364
Thiocapsa floridana (see also bacteria), 956
Tidal marsh (see also salt marsh), 278, 302, 311, 381, 520, 554, 768, 803, 807,
    808, 810, 811, 817, 942, 949, 950, 960, 991, 993-998
Tilapia spp., 582, 867
Tinicum Marsh, PA, 310, 562, 563
Tissue, plant, 743, 857
Tomatoes, 411, 690
Touch-me-not, 993
Toxaphene, 801
Toxicology, 1, 77, 78,  206, 280, 546, 963, 1001
Toxins, 357, 359, 476,  535, 540, 592, 848, 963, 984, 1015, 1032, 1040, 1046,
    1047, 1059,  1080, 1091
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Trace metals  (elements), 87, 132, 136, 233, 234, 278, 280, 357, 399, 400, 548,
    559, 806, 868, 986, 1032, 1045
Transpiration, 663
Transport, 682, 704
Tree(s)  (growth, seedlings), 236, 240, 241, 265, 270, 271, 424, 599, 606, 663,
    667, 669
Triglochin spp., 516
Trophic  levels (see also food chain), 295
Tropical, 332, 383, 566, 594, 759, 761, 834, 900
Trout, 619, 770
Tubers,  516
Tupelo,  574, 606
Turbidity, 250, 499
Typha spp. (see cattail)

Uca pugnax (see fiddler crab, crab)
Unchlorinated effluent (see also chlorination), 247
United Kingdom (see Britain)
United Nations (UNESCO), 682
Upland(s), 513, 872, 1007
Upland swamps, 664, 799
Urea, 583, 942, 953
Urban, 38, 234, 554, 682, 802, 922, 1066
Utah, 199, 616, 634
Utricularia spp. (see bladderwort)

Values,  314, 397, 605, 656, 657, 736, 905, 951, 1085, 1087
Vascular plants (see also plants, macrophytes), 83, 993, 1020, 1096
Vegetation, 53, 85, 106, 180, 197, 265, 270, 271, 429, 433, 436, 454, 463, 479-
    481, 512, 514, 527, 535, 554, 562, 563, 592, 666, 695, 759, 840, 850, 851,
    856, 857, 876, 891, 894, 916, 922, 946, 948, 990, 993, 994, 996, 1052,
    1061, 1096
Vermontville, 329, 873, 876
Vertebrates, 429, 436, 753
Virginia, 149, 210, 541, 720
Virus(es), 4, 65, 72-74, 305, 355, 399, 436, 449, 548, 666, 667, 863, 930, 953,
    974, 975, 977, 978
Volatization, 132, 838
Volunteer wetlands (see also artifical wetlands), 66, 873, 876

Waldo, CA, 673
Walt Disney World, 969
Water budget, 667, 1080, 1087
Water clover (Marsilea quadrifolia), 111
Waterfern, 237
Water-flax seed, 880
Waterfowl (see also ducks), 128, 199, 349, 413, 488, 508, 616, 755, 831, 876
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Water hyacinth, 24, 40, 155, 192,  194,  195,  214,  222,  285,  298,  320,  410,  496,
    543, 551, 565, 582, 594, 597,  601,  621,  628,  674,  723,  747,  751,  766,  793,
    801, 813, 858, 878, 881, 898,  965,  969,  1017,  1021,  1023-1039,  1041-1043
Water lily, 1016
Water-meal  (Wolffia columbiana Karst),  111
Water pollution (see pollution, pollutants)
Water quality, 64, 79, 113, 154, 180,  190, 283, 307, 308,  310,  315,  360,  429,
    438, 449, 454, 458, 501, 517,  518,  651,  798,  808,  843,  850,  873,  994,  1032
Water reuses  (see also reuse), 18,  19,  20, 125, 128, 141,  175,  196,  205,  830,
    874
Water table,  162, 241, 284, 341, 488,  585, 663, 741, 891-897
Water weeds,  1026, 1096
Weeds, aquatic, 766
Wetland evaluation, 730, 736
Wildlife, 32, 129, 175, 180, 221,  403,  525,  543,  608,  666,  722,  823,  922,  971,
    972, 1009, 1071, 1080
Willow, 131
Wisconsin, 35, 36, 247-250, 268, 317,  334, 375, 453, 454,  518,  531-533, 537,
    574, 816, 817, 843, 845, 1002,  1050
Woodland (see also trees, forests), 695,  891-897,  964
Woods Hole Oceanographic Institute, 388,  757-762,  889, 968
World Health Organization, 495, 1049
Wolffia columbiana (Karst) (see water-meal)

Yugoslovia, 943

Zinc, 29, 44, 149, 194, 210, 280,  287,  338,  357,  411,  448,  515,  516,  617,  629,
    690, 805, 806, 942, 986, 1006
Zizania aquatica (see rice, wild)
Zn (see zinc)
Zooplankton, 256, 273, 274, 284, 535

                                           4 U.S. GOVERNMENT PRINTING OFFICE: 1984-756-531/321
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