.ed States
              yironmental Protection
             .yency
Washington DC 20460
             Research and Development
EPA COO 987 009 May 1987
c/EPA     ORD  Annual  Report

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                                  EPA/600/9-87/009
                                  May 1987
 ORD Annual Report
            1986
 Office of Research and Development
U.S. Environmental Protection Agency
      Washington, DC 20460
                      U.S. Environmental Protection Agency
                      K^rion 5, Library (5PL-16)
                      :  ."• S. Dearborn St-eot, ID >-i 1'370

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The Organization
  The EPA's Office of Research and Development
headquarters in Washington does the strategic
planning and budgeting for a program of more than
$250 million in research per year.

  Our research is conducted out of 14 laboratories
located from as far  east as Narragansett, Rl, to as
far west as Corvallis, OR. We have major research
centers in  Cincinnati, OH and Research Triangle
Park, NC. These laboratories report to five major
headquarters offices which are organized along
discipline lines: health; environmental processes
and effects; environmental engineering and
technology; monitoring systems and quality
assurance; and health and environmental
assessment.

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Foreword
  EPA's Office of Research and Development (ORD)
continues to strive for excellence in the Agency's
research program. Because the overall goal of our
program is to provide the scientific and technical
information necessary to support the Agency's
regulatory and enforcement responsibility,
representatives from ORD's client program and
regional offices actively participate on each of our
five research committees (Air and Radiation; Water;
Toxics and Pesticides; Hazardous Waste and
Superfund; and Multimedia Energy). We believe
that the EPA research committee system has
strengthened our ability to plan and manage the
essential scientific and technical work of the
Agency.
  For the second consecutive year each committee
has been asked to prepare an annual report. The
FY-1 986 report is designed to provide the reader
with three things: (1) a summary of major research
issues as defined by each committee; (2) highlights
of major accomplishments that were responsible to
those issues in FY-1 986; and (3) the identification
of related research to be performed during FY-
1 987. The major  purpose of this document is to
acquaint the reader with the interests of each
committee, and to highlight specific research
outputs. We hope that you find the reports
enlightening and useful.
                                                  Vaun A. Newill
                                                  Assistant Administrator for
                                                    Research and Development

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Pesticides and Toxics Committee

Introduction
  Research in pesticides and toxic substances
provides support to meet the current and future
needs of the Toxic Substances Control Act (TSCA),
the Federal Insecticide, Fungicide, and Rodenticide
Act (FIFRA) and, to a limited extent, the Federal
Food, Drug and Cosmetic Act (FFDCA). Research
efforts are geared to provide scientifically valid yet
cost-effective evaluations of the risks associated
with pesticides uses and the manufacture and use
of new and existing chemicals.
  The research program in support of TSCA and
FIFRA develops, evaluates, and validates health and
environmental test methodologies and procedures
to improve the predictability of human risk
estimates, develop exposure monitoring systems,
environmental fate and effects methods, and
guidelines for performing environmental risk
assessments. Additional  research develops and
evaluates release and control methods for new and
existing chemicals, structure activity relationships
as predictors of chemical fate and biological effects,
and procedures for ensuring the human and
environmental safety of the products of
biotechnology. The contamination of ground water
from pesticides is another area of emphasis in the
ongoing research program.


Major Research Issues

ISSUE: Test Method Development
  Under TSCA and FIFRA, manufacturers must test
chemicals and pesticides for potential hazards to
the public health and to the environment.
Consequently, research is needed to provide
guidance for performing such tests. Regulatory
decisions on a chemical depend on  qualitative and
quantitative scientific data from industry regarding
potential adverse environmental and human health
effects of exposure to the chemical. Because
sensitive, reliable, cost and time effective tests are
needed, carefully screened methods under
development include methods for identification of
organic chemicals in products and trace-level
wastes. Such methods will be incorporated into
testing guidelines for use by industry and by those
who must evaluate the safety of chemicals.
1986 Program Accomplishments

Scientific Assessment
  Methods were developed for: (1) deriving factors
for extrapolating male reproductive effects from
laboratory animals to humans; (2) detecting the
spread of antibiotic-resistant bacteria in the
environment; and (3) detecting DNA adducts at
ultratrace  levels. A computer information system
and data collection system for effects of chemicals
on human reproduction was established.

Monitoring
  Analytical methods were developed to identify
and quantitate azo dye chemical compounds in
environmental samples and to support engineering
research on azo dye waste treatment. These
studies employed both tandem mass spectrometry
and Raman laser spectroscopy. Cell biology and
monoclonal antibody techniques were developed to
identify and discriminate chlorinated dibenzo-p-
dioxin isomers.

Health Effects
  In the pesticides health effects area, journal
articles were prepared in the areas of
reproduction/teratology and neurotoxicology. Also,
reports were issued on the role of maternal stress
in the etiology of birth defects, on inhibition of the
expression of oncogenic transformation by certain
pesticide chemicals, on growth enhancement of
human bronchial epithelial cells by nickel sulfate,
and on the use of a renal concentration test in the
neonate as a predictor of long-term alterations in
urogenital morphology. Other reports discussed
test methods to determine the neurotoxic potential
of pesticides. The reports will be used by the Office
of Pesticide Programs for inclusion in testing
guidelines for industry to follow.
  In the toxics area, test methods were developed
to detect the effects of toxic chemicals on the
nervous system, male reproductive system,
developing organisms, and the immune system.
Bioassays to predict carcinogenicity using cell
cultures were  also delivered.

Environmental  Processes
  In pesticides environmental  research, test
methods were developed for early life-stage toxicity

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for Atlantic silversides, Tidewater silversides,
Inland silversides, California grunion, and Gulf
toadfish. Effects were determined for 21 organotin
compounds on survival and growth of marine
unicellular algae. In addition, a handbook of acute
toxicity of chemicals to estuarine organisms was
prepared.
  In the toxics area, several reports addressing
techniques for effects and uptake of sediment-
associated chemicals by benthic marine species
were issued. To answer testing issues related to
species  sensitivity and surrogate species testing,
other reports covered the relative susceptibility of
fish and aquatic invertebrates to reactive chemicals
and the  use of the fathead minnows as a surrogate
for other aquatic species. Research established the
estuarine species, Cyprinodon variegatus, as a
carcinogen assay organism, identified three hepatic
neoplasia types and developed similar systems for
three fresh-water species.


FY 1987 Program Outlook

Scientific Assessment
  In FY  1987, research will focus on the
development of quantitative dose-response models
for developmental and reproductive toxicology, on
methods for detecting dose-rate effects of
chemicals in mutagenesis, and the validation of
human mutagenicity assays in clinical
epidemiology.

Monitoring
  Research will be conducted on methods
development for use in exposure monitoring
research. Immunological techniques will be applied
to exposure monitoring and dose measurements.
Chemometrics will be studied for complex samples
analysis. Efforts will be made to produce standard
procedures for these techniques.

Health Effects
  In FY  1987, test methods development in support
of FIFRA will continue  in the areas of reproduction/
teratology, neurotoxicology, genetic toxicology and
immunotoxicology. Research emphasis in the area
of immunotoxicology is a priority area for the Office
of Pesticide Programs.
  In toxics research, neurochemical, behavioral,
and physiological methods for detecting
neurotoxicity will be provided. Methods for
predicting developmental and reproductive toxicity
and germ cell mutation will continue under
development. Methods will also be delivered
evaluating the impact of chemicals on immune
function, predicting the skin absorption of
chemicals, and evaluating the effect of chemicals
on microflora of the gastrointestinal tract. Methods
for evaluating tumor initiators and promoters will
also continue to be developed.

Environmental Processes
  Research in the pesticides area will develop and
validate bioassay methodologies for determining
the effects of chemical pesticides on estuarine and
freshwater animals. Methods will be made
available to test fishes and crustaceans and other
pesticide sensitive organisms. Environmental
factors which may modify organism response will
be determined so that proper interpretations can be
rendered. Impacts on acute or chronic tests such as
toxicity-time exposure relationships, sediment-
water interactions and salinity will be examined.
The new methods and test organisms may become
monitoring tools providing the opportunity to make
more reliable decisions when regulating.
  Toxicity test methods will be developed for
evaluating hazards associated with toxic chemicals
to aquatic and terrestrial species. These methods
will be used for evaluating new and existing
chemicals under Sections 5 and 4 of TSCA,
respectively. Some of the major methods  which will
be studied include tests for bioavailability for
evaluating sediment bound toxicants for freshwater
and estuarine organisms; tests to determine
toxicant effects on avian egg viability and methods
to use fish as surrogate assays for determining
carcinogen and teratogen potentials in other life-
forms.

ISSUE: Structure Activity Relationships
        (SAR)
  To enhance the efficiency of the regulatory
process for toxic substances, it is convenient to
group various chemicals which share common or
similar chemical characteristics rather than to deal
with each individual chemical. If it can be
demonstrated that chemical relationships, such as
similar molecular structures and similar modes of
toxic activity, form a firm scientific basis for
estimating probable environmental risks, then
better guidelines and techniques can be applied
and regulatory actions can be completed more
quickly using  less resources. SAR is vital for
reviewing and screening PMN chemicals  under
Section 5 of TSCA. The findings  and techniques
established in this research are used to select
appropriate toxicity tests, to document test results,
to develop fate and effects data bases where
necessary and to provide the modeling means to
predict toxicity.

FY 1986  Program Accomplishments

Health Effects
  A rapid, inexpensive method for calculating
molecular electrostatic potentials was developed to

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aid in predicting the toxicity of chemicals.
Additional data were provided on the enzyme
azoreductase to aid in predicting the toxicity of azo
dyes.

Environmental Processes
  Three advances on SAR application have been
made available. Methods have been devised for use
which predict the mode of toxic  action for PMN
chemicals, which provide a comprehensive
structure-toxicity method to estimate chemical
toxicity to aquatic organisms, and which function to
predict major metabolites of  industrial chemicals.
SAR applications were reported on identification of
potential tumorogenic industrial chemicals and a
knowledge base expert system for managing false
negatives m  chemical evaluations was delivered to
OTS. Relationships between microbial rate
constants and chemical properties have been
published.


FY 1987 Program Outlook

Health Effects
  Studies will continue to explore which azo dyes
will be converted by azoreductase to more toxic
compounds.  Toxicological data will be gathered on
specific chemical classes such as acrylates and
pyridines. Computer-assisted methods for relating
mutagenicity to chemical structure and a data
management system for genotoxic chemicals are
being developed.

Environmental Processes
  Structure activity relationships, used for
evaluating PMN chemicals under TSCA, Section 5,
will be developed for determining the fate and
toxicity of toxic substances in the environment.
While prediction of metabolites  using the SAR
concept will  be included, emphasis will be on
developing fate  relationships for predicting
photolysis and biodegradation. Estimation
parameters which incorporate property/molecular
reactivity will consider metabolic factors,
bioaccumulation, and persistence. Compound
structure influences on microbial rate constants
and microbial transformation mechanisms will be
described.

ISSUE: Special Human Data Needs
  To improve the Agency's ability to estimate risks
to human  health, research activities in FY 1987 will
examine population groups exposed to suspect
toxicants in the environment to  determine if
biological indicators of dose and/or effects are
related to environmental levels of exposure. Data
are also being developed on  adverse effects as
measured by traditional epidemiological studies.
FY 1986 Program Accomplishments

Health
  Support was provided for examining the potential
for using biological monitoring to estimate
exposure to toxic chemicals.
FY 1987 Program Outlook

Health
  Research will focus on biochemicals and
environmental epidemiology with emphasis on
better methods of assessing exposure and effects.


ISSUE: Ecology—Transport/Fate/Field
        Validation
  To adequately evaluate the likely perturbations a
pesticide or toxic chemical may cause in the
environment, it is necessary to understand
probable exposure concentration/durations,
movements through ecosystems, degradation rates,
reservoirs, effects and residues. The Agency  must
have applicable techniques to attain this
information; must be able to interpret findings and
have a capacity to predict problems. Research in
this area is designed to improve the criteria and
standards against which users of the toxic
materials and the Agency must comply. The intent
is to provide new or improved state-of-the-art
techniques to fill data gaps in order to have
scientifically credible and legally defensible
regulatory actions.

Environmental Processes
  In the pesticides research area, findings of three
important areas relating to field studies were
transferred to program offices. Field effects of
methyl parathion to terrestrial species were
determined.  Proposals were issued on how to test
the limits of applicability, i.e., methods results
laboratory vs. field comparisons and how to relate
field to laboratory data to improve hazard
evaluations in coastal/marine systems. Methods
were field validated for predicting the effects of
Abate on freshwater non-target organisms.
Exposure determinations from the annual
Dougherty Plain site study results were compiled
and distributed and simplified exposure procedures
(nomographs; tables) based on HSP-F model
production runs were transferred in usable form to
OPP in the form of a manual.
  In the toxics program, marine studies determined
the influence of scale on measured results from
laboratory microcosms subjected to toxic
chemicals. Field studies were used to verify the fate
and effects resulting from microcosm experiments.
Fate screening tests were applied to collect

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biodegradation information which was used to
predict the fate of toxic organic compounds.
  Freshwater system investigations were
completed and an evaluation of a pharmacokinetic
model offish, used as a surrogate species, for
mammals for initial screening of PMN chemicals.
The application of Leffler and Taub microcosms
was evaluated for screening chemicals and
information was released on SAR applications on
microcosms at various ecosystem levels from single
species to community level toxicant reactions.
  Also, research results were released on
environmental factors which govern the kinetics of
abiotic redox transformations and on light-induced
oxidation of inorganic substances, as were the
study results of the model TOXIWASP applied in an
exposure study of organic substances in the
Delaware River.
  Terrestrial system studies have determined the
effects of cold stress on avian  acute toxicity tests in
an effort to reduce test result variability.

FY 1987 Program Outlook

Environmental  Processes
  Pesticides research will concentrate on the
development, refinement and validation  of
techniques and models to predict transport,
degradation and fate, as well as exposure estimates
of pesticides through surface and subsurface
matrices. Field and laboratory studies will be
conducted to determine the validity of laboratory
methodologies and if the results reflect
environmental responses  under natural conditions.
Laboratory and field data will be used to assess
pesticide hazards to surrogate species and
populations and to provide information on pesticide
sorption, leaching and residues. The environmental
impact to ground-water caused by pesticides and
other agricultural practices will be investigated.
Pesticide processes in soils and ground-water will
be characterized, information will be developed on
spatial variability in subsurface cores, and
evaluations will produce best management
practices which could mitigate ground-water
contamination by pesticides.
  Toxic substances research is designed to provide
methods, approaches and information for three
evaluative areas concerned with field validation,
exposure to toxicants and risk determinations. Field
and lab related activities will include: (1)
development of comparative toxicological
correlations for identifying surrogate species used
in toxicity testing;  (2)  development of assessment
techniques and their evaluation in field sites for
assessing hazards to  ecosystems and biota; and (3)
development of fate (such as biodegradation) and
exposure mathematical models to evaluate or
predict exposure and  hazards associated with toxic
substances in the environment. Research will
attempt to put limits on or define uncertainty of
predictive methods and provide user guides for
those models verified and/or ready for regulatory
application. System-level effects studies will define
adverse effects of toxic chemicals on system
processes and functions. Investigations will focus
on multispecies and community level methods and
data generation. Application of multistage
microcosms will be enlisted to  enhance verification
procedures of methods, models and hazards.

ISSUE: Health—Markers, Dosimetry, and
        Extrapolation
  For both the pesticides and toxic substances
programs, health effects research in FY 1986
focused on development of methodologies for
extrapolation of data from high  to low doses and
between mammalian species to enhance  human
health risk assessment predictability. In the toxic
substances research program, particular emphasis
is being placed on studies which involve defining
the relationship between biological markers of
exposure to neurotoxicants and behavioral
dysfunction as well as studies in dosimetry and
extrapolation related to genetically mediated  health
effects. Additional pesticides, research includes
evaluating the relationship(s) between age and
dermal absorption using in vivo animal  models as
well as research on compound-induced
reproductive alterations following exposure during
developmental periods. Data generated are used to
extrapolate toxicant risks to humans.

Health Risks
  Journal articles on extrapolation methodology
were prepared on percutaneous absorption of
Folpet in young and adult rats in vivo and in vitro
and on age-related skin penetration of three
insecticides in rats in vivo and in vitro.
  A gene-tox carcinogenic data base was also
developed to help determine those bioassay
systems which best predict carcinogenicity. The
dose-effect relationship of asbestos and other
durable mineral fibers was provided to  the Office of
Toxic Substances in support of the ban and phase
down of asbestos.

FY 1987 Program Outlook

Health Effects
  In FY 1987, a number of methods for
extrapolating neurotoxic  risks from animals to
humans will be investigated. Other research
projects will study in vitro systems to predict  the
tumorogenicity of chemicals.  Methods will be
developed for detecting functional effects of
prenatal exposure to toxicants.

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  Research will also continue in the areas of
genetic toxicology, neurotoxicology and
reproduction/teratology. Research will focus on the
application of the parallelogram extrapolation
approach for pesticides, on the effects of
formamidenes on visual evoked potentials, and on
comparison of in vitro and in vivo skin absorption of
dinoseb in rats.


ISSUE: Exposure Monitoring
  The major TSCA related monitoring research
efforts are directed toward improvements in
monitoring systems to estimate total  human
exposure. Research  is conducted to develop
approaches for multimedia/multipathways of
pollutants to estimate total human exposure. This
entails development of questionnaires and human
activity pattern analyses to identify key population
segments which may be exposed to chemicals,
investigation of environmental dose and personal
monitoring devices,  providing more accurate
measures of the different exposure routes of
pollutant intake, and incorporation of network
monitoring systems  with total human exposure
monitoring through studies conducted in the
human Exposure Assessment Location Project and
research at the  Environmental Methods  Test Site.
Additionally, research is conducted on chemicals
and biological techniques to identify and quantify
pollutants in biological tissues and fluids.


FY 1986 Program Accomplishments

Monitoring
  Research for exposure monitoring  in FY 1 986
focused on implementation of the Environmental
Methods Test Site (EMTS) and the Human Exposure
Assessment Location (HEAL) Project. At  EMTS,
construction of the base map for the  geographic
information system (CIS) was defined and initiated
and the quality assurance plan for the project was
produced. The draft protocol and the  quality
assurance for the  HEAL HCB/DDT pilot was
approved by the participating countries.
Additionally, reports on the use of kriging were
produced and a preliminary computer program for
analysis of human activity patterns was  produced.
FY 1987 Program Outlook

Monitoring
  The HCB/DDT pilot will be conducted at the
EMTS. Work on development of a users guide for
the CIS will continue. Statistical research on
human activity pattern analysis using TEAM study
results will be conducted.
ISSUE: Biotechnology/Microbial and
        Biochemical Pest Control Agents
  Many of the techniques required to adequately
control or regulate microbial organisms or
"biochemical" products (e.g., pheromones) apply to
both TSCA and FIFRA mandates. Beyond these
basic techniques, however, there is a divergence—
microbial applications under TSCA are usually
industrially oriented and relate to workplace
exposure or accidental releases; the microbial
applications under FIFRA are an intentional
dispersion to control undesirable flora or fauna.
Such microbial pest control agents (MPCA's) may
be "natural" selected stock or may be genetically
altered.
  Users of biotechnological products must follow
recommended Agency guidelines in a testing
regime designed to help prevent adverse
environmental impacts. ORD helps establish these
techniques, determines if environmental effects are
exhibited by previously untested non-target
organisms and conducts field-oriented validation
studies as necessary to insure that testing criteria
and guidelines are appropriate and functional.
Engineering research is also underway to develop
and/or improve methods to contain or destroy
genetically engineered organisms.
  Under FIFRA,  research is geared towards
developing or improving bioassay methodologies for
determining the effects of biological control agents
(BCA's) on non-target receptors or hosts. This
research includes testing protocols and effects
information for unaltered and genetically altered
microbial BCA's. Investigations focus on routes of
exposure, methods to detect and identify agents,
toxicity, infectivity, persistence and effects. The
resultant information will be used to revise subpart
M guidelines and to guide regulatory decisions in
pre- and post-registration actions.
  Pesticides health research  in  biotechnology
involves development of data on the immunologic
effects of microbial pesticides on mammalian cells.
Also, methods are being developed for using
monoclonal antibodies and biotinated DNA probes
to identify genetic material from biological
pesticides in non-target sites such as mammalian
cells. Such methods will provide the basis for
validation subpart M guidelines for testing
microbial pesticides.
  Under TSCA, research focuses on development of
scientific rationales and procedures for evaluating
the environmental survivability, reproduction,
distribution, effects and risk associated with the
escape of genetically manipulated organisms. The
research results will be used to prepare protocols
for use in evaluating TSCA products involving
environmental application of microbes. This
research will also support  regulatory rule-making

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specifying which products are to be considered
under TSCA.
  In the toxic substances health research area,
studies are being conducted to determine the
genetic stability and function of a baculovirus
expression vector in vertebrate cells and to
evaluate the health effects of species and strains of
genetically altered organisms.

FY 1986 Program Accomplishments

Health Effects
  A report on characterization of Bacillus
thur/ngiensis Cytolytic Factor was prepared. This
report will be included in the Office of Pesticide
Programs' testing guidelines. Work also continued
on developing assay methods for studying the
genetic stability and gene expression of a
baculovirus constructed to express foreign gene
products. Efforts were also directed toward better
characterizing microorganisms that degrade
hazardous compounds in order to use these
organisms in future studies to evaluate the health
effects of mutant and recombinant organisms.

Environmental Processes
  A report was published on a FY 1 986 workshop
on "Enclosed Systems for Testing Microbial Pest
Control Agents." In order to  assess the fate and
effects of non-target marine species of viruses,
bacteria, fungi, and protozoa that are potential
biorational agents, a study was completed where
shrimp were exposed to an insect virus and
histological and serological examinations were
carried out. Also, a report was published on field
validation  of tests techniques for biological control
agents of freshwater organisms and a progress
report on the hazard assessment of biochemical
agents on  metamorphosis and growth of marine
crustaceans.
  Two other published reports increase the
Agency's ability to obtain and interpret data on new
biotechnology products. The reports describe a data
base to assess the genetic stability of novel
organisms and a data base for detecting, identifying
and enumerating novel organisms  in terrestrial
environments. Several journal articles describe
techniques for evaluating regrowth and persistence
of genetically engineered bacteria released to
terrestrial  ecosystems, evaluation of techniques for
the detection and enumeration  of transconjugants
in laboratory media, and the fate and survival of
recombinant bacteria and recombinant DNA in
insects.

FY 1987 Program Outlook

Health Effects
  Research will continue in  methods for the
identification and characterization  of microbial
agents. This work will include recombinant of DNA
in plasmids and genome of bacilli, and on the
competition of endogenous and non-endogenous
intestinal microorganisms. Research will also
continue on the ability of non-endogenous
microorganisms to compete with endogenous
organisms of the gastrointestinal tract and the
implications of this to human health. The potential
for genetic exchange between organisms will also
be investigated.

Environmental  Processes
  Research will be conducted to develop or improve
bioassay methodologies for determining the effects
of biological control agents (BCA's) on non-target
receptors or hosts. This includes providing testing
protocols and effects information for unaltered and
genetically altered microbial BCA's and the study of
biochemical and genetically altered agents.
Investigations will focus on routes of exposure,
methods to detect and identify the agents and the
toxicity-infectivity, persistence and effects. Major
research will commence on biotechnological
applications, e.g., on genetically altered microbial
control agents. Studies will discern  how to handle,
recover and monitor these organisms and how to
determine probable impacts on non-target
organisms in the aquatic and terrestrial
environment.  This research defines areas of
concern and will result in more rapid and greater
public acceptance of safe reliable bioengmeered
organisms and substances. BCA testing
requirements, movement and survival in
freshwater systems and biochemical and  insect
fungi BCA hazards to marine  crustaceans will be
determined. The information  will be used for
improving subpart M guidelines  and for regulatory
decisions in pre- and post-registration actions.
  FY 1987 research will also  address the need for a
credible system for analyzing the potential
environmental hazards of genetically modified
microbes. This includes laboratory and field or
microcosm studies to modify  procedures for
identifying, enumerating and tracking  engineered
organisms or genes in environmental situations.
Factors which will be studied in  the aquatic and
terrestrial media are persistence, detection,
potential for gene transfer and fate and effects.
Research should provide testing protocols and
provide information and guidance on hazards and
on approaches to assessing those hazards. This will
support regulatory rule-making for TSCA products
which  involve environmental application of
microbes.

ISSUE: Engineering  Release and Controls
   Under the premanufacture notification (PMN)
process, manufacturers are required to submit

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information to EPA on the release and control of
new chemicals and significant new uses of existing
chemicals. EPA uses existing data to predict the
risks of and from the release of new substances;
and under the existing chemicals control program
evaluates technological alternatives to reduce the
release of and exposure to chemicals that are
already in use.
  Models are being developed which can predict
release of and exposure to classes of new
chemicals for assessment of chemical-unit
operations and processes, and the physical and
chemical properties of chemical substances.
Additionally, models to predict potential exposure
and release levels, and the best control measures
to control release of an exposure to new chemicals
are being developed. Treatability testing of
potentially toxic chemicals are also conducted.
  Alternatives to mitigate the release of and
exposure to specific existing and new toxic
substances are being defined through the
evaluation and adaptation of control measures
related to the release of chemicals in the workplace
and into the environment. Technologies,
management practices, and personal protective
equipment to limit the release into the
environment, and exposure to those toxic
substances, are being evaluated.
  Under the Federal Insecticide, Fungicide, and
Rodenticide Act, EPA is responsible for pesticide
exposure studies, for reviewing and approving
pesticide labels, for administration of the pesticide
Farm Safety Program, and for supporting training
and education programs for pesticide users through
state extension services. The Agency is concerned
that protective clothing currently recommended for
use by pesticide users is not providing acceptable
protection. This situation is aggravated by a lack of
appropriate data. In order to improve the situation,
EPA requires  greatly improved documentation
regarding the effectiveness of protective clothing.
This program  focuses on generating breakthrough-
time and steady-state permeation rate data for
concentrated  formulations of high toxicity
pesticides through a range of commonly available
polymer gloves that may be suitable for use by
mixers and  loaders of pesticides. Evaluations of the
job compatibility and degree of protection provided
by clothing items other than gloves are also being
conducted in  laboratory and field testing.


FY 1986 Program Accomplishments

Engineering
  A report entitled, "Interim Guidance Manual for
Selecting Protective Clothing for Agricultural
Pesticide Operations" was completed in FY 1 986.
This report provides information needed by the
Office of Pesticide Programs to make acceptable
the protection provided by protective clothing for
workers exposed to pesticides. Also, preliminary
assessment was conducted on predictive
techniques for extraction, flaking, and
agglomeration unit operations. Interim reports were
completed on the development of validation of
predictive models and test methods for estimating
the effectiveness of protective clothing for
chemicals, and laboratory testing on half-mask
respirators. Evaluations of procedures and
methodology of asbestos abatement were also
conducted.
FY 1987 Program Outlook

Engineering
  Evaluations of polymer gloves for agricultural
pesticide use are currently being conducted, and
will be continued. Methodologies for estimating
protective clothing performance will be evaluated
for PMN review process. Full-scale piece
respirators are being tested in the laboratory.
Research on asbestos abatement will cover
evaluations of plastic barrier effectiveness, glove
bag removal, and localized vacuum removal, and an
assessment  of effectiveness of O&M guidance
provided for  asbestos abatement.

ISSUE: Ecology—Ecotoxicity and Risk
        Assessment
  In the past, emphasis in ORD's scientific
assessment  program has been placed on the
assessment  of risk to human populations. However,
there  is also a need to assess the risk to non-
human populations and the environment. The
development of ecological risk assessment
protocols and guidance for terrestrial and aquatic
ecosystems (primarily endangered species and
commercial fisheries) is necessary to quantify the
probability that adverse effects may occur as a
result of exposure to a toxic substance and to
estimate the significance of such effects in the
environment. Since environmental data developed
by industry may vary greatly from chemical to
chemical, procedures need to be developed which
provide guidance and consistency for the various
environmental exposure activities. This work will
develop risk  assessment protocols and guidelines
for the assessment of adverse effects on terrestrial
and aquatic ecosystems.

FY 1986 Program Accomplishments

Scientific Assessment
  Studies were initiated to develop procedures for
performing environmental risk assessment.

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Environmental Processes
  The initial design of a computerized terrestrial
model was completed, as was a survey of existing
biological databases covering susceptible species.
As part of  an effort to integrate information from
toxicity, biochemistry, and physiology with
ecosystem models, a report was prepared
describing a comparative physiology database for
use in identifying susceptible populations. In
addition a  report describing suitable methods for
estimating the probability of ecological damage was
prepared. Also developed was a component of the
Decision Support System, a gill exchange model
which predicts the exchange and bioaccumulation
of hydrophobic chemicals. The model has been
computerized and a  user's manual has been
completed.


FY 1987 Program Outlook

Scientific Assessment
  Guidelines for risk assessment for terrestrial and
aquatic ecosystems  focusing on endangered
species and commercial fisheries will be
completed.

Environmental Processes
  FY 1 987 studies will develop ecological risk
models and integrated risk assessment processes
which express probability of risk to important
ecological populations. These studies include
combining exposure and hazard methods and data
and utilizing modified existing or new fate,
transport and effects models to depict risk. Levels
of uncertainty coupled with these approaches will
be described. Extrapolation of data across
populations and multiple usage of information will
be initiated to identify reliable endpoints.
Integration of models, output and other information
will allow ecosystem degradation criteria to be
developed. Some key factors to be addressed are
ecosystem stability,  resiliency, stratification, kinetic
changes or exchanges and transport and fate.
Substantial modeling will address multimedia risks
and the models will  be subtended with appropriate
software packages.

ISSUE: Support
  For certain assessments, because data are
lacking, the ORD staff is required to conduct
literature  searches,  interpret data or render
technical  and scientific judgments. In cases where
program office evaluations are complicated and/or
controversial, independent peer review of
assessments are required to ensure consistency.
There is a continuing need for ORD participation in
and review of major exposure and hazard
assessments conducted by OPTS which provide
Agency policy makers with technical expertise from
qualified scientists, and for improving Agency
regulatory decisions. Finally, in both the pesticides
and toxics areas, support will continue for quality
assurance and maintenance and dissemination of
standard reference materials. This entails the
production of pure chemicals in solvent solutions or
in environmental media to ensure the precision and
accuracy of routine analyses as well as the
production of reference spectra to identify unknown
chemical compounds in routine environmental
monitoring.

FY 1986 Program Accomplishments

Scientific Assessment
  Detailed assessments in the areas of
carcinogenicity, mutagenicity, reproductive toxicity
and exposure on a variety of chemicals were
delivered to the program offices. Additional support
was provided by ORD personnel serving as expert
witnesses in litigations and  in review of program
office proposed test rules and pesticides review.

Monitoring
  A 1985 annual report (EPA Pesticides and
Industrial Chemicals Repository) summarized the
activities of the repository and its continuing
mission to provide a source  of analytical reference
materials. This program helps to insure a single
common source of certified  standard samples
necessary for good quality control. Samples are
supplied to and used by federal, state, local and
academic laboratories. The report indicates the
distribution of 30,000 samples to over 1,400
laboratories each year and includes information by
type of laboratory, types of standards (samples) and
by country.
  A 1985 annual report (Intercomparison Program
for Pesticides) described a study program which
tests the ability of various laboratories to perform
pesticide analysis for environmental monitoring
purposes.  This study evaluated the OPP contract
laboratories and identified a need for continued
monitoring of all laboratories engaged in
measurement and analysis. Average analytical
results need to  be improved. Also conducted was
the annual bulk audit program for analysis of
asbestos from building  materials and insulation; a
report of the results was produced. The  need for
quantitative analysis of these materials  was
investigated. An audit program for airborne
asbestos identification was started by development
of a standard transmission electron microscope
method for asbestos analysis.

Environmental Processes
  Technical assistance was  provided to OTS by
ORD in areas requiring special research  aid or

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scientific expertise necessary for TSCA
implementation. This assistance enhances both
regulatory action(s) and program management to
insure that deliverables are appropriate to needs
and priorities. Another completed TA project
determined the chronic toxicity of phthalate esters
to two test organisms, Daphnia and fathead
minnows.
FY 1987 Program Outlook

Scientific Assessment
  Detailed assessments in the areas of
carcinogenicity, mutagenicity, reproductive toxicity
and exposure on a variety of chemicals were
delivered to the program office. Additional support
involved serving as expert witnesses in litigations
and review of program office activities including
proposed test rules, and special review of
pesticides.

Monitoring
  A program will be maintained to provide quality
assurance/quality control to laboratories engaged
in pesticides monitoring activities. This will provide
the prevision and accuracy for analytical and other
data to support Agency requirements.  Secondly, the
repository for standard chemicals and  its
distributional function will continue to supply high
purity certified samples to users.
  A study will also  be conducted in an effort to
produce quality assurance chemicals which match
program office needs more readily than current
procedures. The airborne asbestos audit program
development will be increased to develop a
standard analytical method this year.

Environmental Processes
  Technical assistance will be provided to OTS on
complex problems relating to environmental fates,
exposure, effects and risks of toxic chemicals or
bioengmeered organisms necessary for
implementation of TSCA. The forms of technical
assistance to be accomplished include workshops
and peer and other reviews and provision of special
data or information which is not otherwise
available. This includes toxicity tests and support
for exposure and risk  modeling and assessments.

Test Method Development

1986 Program Accomplishments

Monitoring

• Report on application of analytical methods for
  detecting organic compounds in biological
  tissues—FY 1986. 12/86
• Journal article on azo dye identification and
  quantitation procedures. 11/86

• Report on application of analytical methods for
  detecting organic compounds in air—FY 1986.
  12/86

Health Effects

• Journal articles on percutaneous absorption of
  folpet in young and adult rats in vivo and in vitro.
  9/86

• Journal articles on age-related skin penetration of
  three insecticides in rats in vivo and in vitro
  studies. 9/86

• Neurophysiological effects of perinatal lead
  exposure in monkeys. 9/86

• The evaluation of neuron-specific proteins as
  biochemical indicators of toxicity. 9/86

• Comparison of in vitro and in vivo testicular
  function. 9/86

• Journal article on role of maternal stress in the
  etiology of birth defects. 6/86

• Cytogenic methods for detecting chromosome
  damage. 9/86

• Journal article on inhibition of the expression  of
  oncogenic transformation by chemicals.  9/86

• Quantification of neurotypic and gliotypic proteins
  for assessing neurotoxicity following toxicant
  exposure. 9/86

• Complete development  of two immunotoxicity
  assays in animals. 9/86

• Journal article on growth enhancement of human
  bronchial epithelial cells by nickel sulfate. 9/86

• Journal article on the use of renal concentration
  test in the neonate as a predictor of  long-term
  alterations in urogenital morphology. 9/86

• Report on the dose-response curve for 1,2
  dibromoethane. 8/86

• Testing protocol for rapid and cost-effective
  assessment of reproductive damage. 9/86

Environmental Processes

• Report on  critical responses of populations of
  Crustacea to toxicants. 12/85

• Report on  reproductive biology of Menidia
  peninsulas. 12/85

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• Report: Handbook of Acute Toxicity of Chemicals
  to Estuarine Organisms. 9/86

• Report on techniques for effects and update of
  sediment-asso. tox. chemicals by Benthic Marine
  Species. 6/86

• Report on the fathead minnow as a surrogate for
  other aquatic species. 7/86

• Report on use of relative, position-specific eff.
  measure, of benzo(a)pyrene metabolism as field
  indicators of carcm stress aquatic environ. 9/86

• Report on analysis of several tumor types exper.
  induced Sheepshead minnow and their signific.
  to mammalar lesions. 9/86
Structure Activity Relationships (SAP)

1986 Accomplishments

Health Effects

• Report on how to use molecular electrostatic
  potential to make risk assessments on
  chemically induced toxicity for one class of
  chemicals. 9/86

• Report on SAR studies on azoreductase II. 9/86
Environmental Processes

•  Report on SAR methods to predict mode of toxic
   action for PMN chemicals. 10/85

•  Report on a comprehensive structure-toxicity
   method to estimate toxicity of chemicals to
   aquatic organisms. 3/86

•  Delivery of a knowledge-base expert system for
   managing false negatives in chemical
   evaluation 6/86

•  Report on SAR methods to predict major
   metabolites of industrial chemicals. 8/86

•  Report on SAR identification of potential
   tumorogenic industrial chemicals. 9/86
Special Human Data Needs

1986 Program Accomplishments

• Proceedings of conference on medical screening
   and monitoring for effects of chemical exposure
   in the workplace. 9/86
Ecology: Transport, Fate and Field
          Validation

1986 Program Accomplishments

Environmental Processes

• Final Report on Field Effects of Methyl Parathion to
  Terrestrial Species. 12/85

• Draft Users Manual—Simplified Exposure
  Procedures (nomographtables) to OPP  Based on
  HSP-F Production Runs. 1/86

• Report on hazard evaluations and relate field to lab
  data with proposal on how to test limits of
  applicability. 2/86

• Report on field validation for predicting effects of
  Abate (Temephos) on non-target organisms.
  6/86

• Dougherty plain project report. 9/86

• Report on pharmacokmetic model of fish as a
  surrogate species for mammals in initial
  screening of PMN chemicals. 10/86

• Report on field validation of fate and effects of
  selected toxic chemicals derived from laboratory
  microcosms. 12/85

• Journal article on environmental factors
  governing the kinetics  of abiotic redox.
  tranformation. 12/85

• Journal article on light-induced oxidation of
  pollutants involving natural inorganic species.
   12/85

•  Progress report on evaluation of Leffler & Taub
   microcosms for screening chemicals. 1 /86

•  Report on effects of cold stress on avian LD-50 and
   LC-50 tests. 2/86

•  Report on the influence of scale in  marine
   microcosms perturbed by toxic chemicals derived
  from laboratory microcosms. 3/86

•  Journal article on exposure studies of organics in
   Delaware River using TOXIWASP.  4/86

• Journal article on SAR at the ecosystem levels
  single: species relative tox. vs. ecosystem level
  tox. using microcosms. 8/86

• Report on suitability of biodegradation information
  from fate screen tests for pred. fate of tox.  organ.
  compounds in microcosms and field studies.
  9/86
                                               10

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Health: Markers, Dosimetry and
        Extrapolation

1986 Program Accomplishments

Health Effects

• Report on test methods to determine the
  neurotoxic potential of pesticides. 1 2/85

• Journal articles on development of new
  techniques to evaluate  heritable gene mutations.
  9/86

• Predictive models for dose effects of asbestiform
  minerals. 9/86

• Journal article on the review and analysis of the
  literature of select chemicals and the
  establishment of the gene-tox data base. 7/86

• Journal article on metabolism of benzo(a)pyrene in
  monolayer cultures of human bronchial
  epithelial cells from a series of donors. 3/86

• Journal article on mterspecies comparison of
  adult to developmental  toxicity ratios. 8/86

• Effects and interaction of metals and metal
  chelators on  aversion learning. 3/86

Exposure Monitoring

1986 Program Accomplishments

Monitoring

• Annual report on research activities and methods
  development at the EMTS. 12/86

• Interim report on pollutant sources and human
  exposure to toxic pollutants using TEAM data—
  FY 1986. 12/86

Biotechnology/Microbial and
Biochemical Pest Control Agents

1986 Program Accomplishments

Health Effects
• Characterization of Bacillus thuringiensisc\\.o\\i\\c
  factor. 9/86

Environmental Processes

• Summary Report: Exposure Studies of Insect
  Virus, Bacterium, and Protozoa and Fungus on
  Non-Target Estuarme Crustacea. 11 /85

• Report on field validation of test techniques for
  biological control agents on freshwater
  organisms. 1 2/85
• Report on workshop for ORD/OPP entitled:
  Enclosed Systems for Testing Microbial Pest
  Control Agents. 6/86

• Progress report on hazard assessment of
  biochemical agents on metamorphosis and
  growth of marine crustaceans. 9/86

• Progress report on the effects of insect fungi in
  Crustacea. 9/86

• Report on data base for detecting, identifying, and
  enumerating novel organisms in terrestrial
  environments. 1/86

• Report on development of data base assessing
  genetic stability of novel organisms. 2/86

• Internal report on regulatory res. for biotech:
  identification and enumeration techniques,
  survivability and genetic exchange in genet.
  altered microorg. 9/86

Engineering Release and Controls

1986 Program  Accomplishments

• Interim guidance manual for selecting protective
  clothing for agricultural pesticide operations.
  9/86

• Preliminary assessment of predictive techniques
  for extraction, flaking, and agglomeration unit
  operations. 1/86

• Interim report on the development of validation of
  predictive models and test methods for
  estimating the effectiveness of protective
  clothing. 5/86

• Interim report on laboratory testing on half-mask
  respirators. 9/86

• Report on the fate of azo dyes m activated sludge
  process. 10/86

• Evaluations of procedures and methodology of
  asbestos abatement.  3/86

Ecology: Ecotoxicity and Risk
          Assessment

1986 Program  Accomplishments

Environmental  Processes

• Report describing design specifications for models
  including quantitative uncertainty estimates.
  7/86

• Report on comparative physiology data base for
  the  identification of susceptible species. 9/86
                                              7 1

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• Report on components and characteristics of
  prototype terrestrial exposure model. 9/86

• Report on functional objectives and
  recommendations of prototype computer system.
  7/86

• Journal article on model developed to describe
  exchange of non-polar hydrophobic toxicants
  across fish gills. 8/86

• Computer program of gill exchange model for
  predicting bioaccumulation of hydrophobic
  xenobiotic chemicals. 9/86

Support

1986 Program Accomplishments

Monitoring

• Annual report on intercomparison program for
  pesticides. 7/86

• Annual Report: EPA Pesticides and Industrial
  Chemicals Repository. 5/86

• Guidelines for field testing soil, data, and transport
  models—final report.  4/86

• Report on asbestos audit program. 9/86

Environmental Processes

• Final report on chronic toxicity of phthalate esters
  to Daphnia and fathead minnows. 3/86


Test Method Development

FY 1987 Program Outlook

Monitoring

• Report on application of analytical methods for
  detecting organic compounds in biological
  tissues—FY  1987. 12/87

• Report on application of analytical methods for
  detecting organic compounds in air—FY 1987.
  12/87

Health Effects

• Journal article describing validated test protocol to
  detect and characterize neurotoxicity. 10/86

• Report on age-related neurotoxic sensitivity to
  organophosphate induced delayed neurotoxicity.
  10/86

• Report on germ cell cytogenetic test systems.
  12/86
• Glutathione Synthesis: A Requirement for Sperm
  Nuclear Decondensation During Oocyte
  Maturation. 3/87

• Maternal Toxicity of Methoxychlor and Dibutyl
  Phthalate. 2/87

• Neurobehavioral  Assessment of Egyptian
  Pesticide and Textile Workers. 1 2/86

• Pyrethroid Effects on Schedule-Controlled
  Behavior: Time and Dosage Relationships
  12/86

• Behavioral aiid Neurochemical Effects of Amitraz
  Exposures in Rats. 6/87

• Some Behavioral Effects of Triphenyltm. 3/87

• Embryonic Dosimetry of Putative Teratogens.
  8/87

• Comparison of the Behavioral Effects of
  Endosulphan in Juvenile and Adult Rats. 9/87

• Carcinogenic Initiators and Promoters' Report on 4
  Agents. 2/87

• Test System  for Evaluating the Effect of
  Environmental Chemicals on Metabolism by
  Microflora of the  Gastrointestinal Tract. 9/87

• Report on prediction of skin absorption with the
  rotating diffusion cell (RDC) model. 9/87

• Modification of Aversion Learning: A Behavioral
  Assay for Studying  Metal-Chelator Interaction.
  6/87

• Report on developmental toxicity of phenolic
  congeners. 9/87

• Report on immune function in young adult  mice
  exposed to 2-deoxycoformycin in utero. 12/86

• Evaluation of the immunotoxic effects of di-n-
  octyltin-dichloride in the rat. 2/87

• Evaluation of seizure models as indicators of
  neurotoxicity. 8/87

• A sound stimulation system for auditory research
  with small research animals. 9/87

• Report on detection and quantification of nervous-
  system protein in cerebrospinal fluid in toxicant
  exposed rats. 9/87

Environmental Processes

• Report on optimization of environmental factors
  during the life-cycle of Mysidopsis bahia. 1  2/86
                                               12

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• Final Report: Methods Manual for Spawning,
  Culture and Testing of Atherinid Fishes. 1 2/86

• Report on toxicity-time relationships for fish
  exposed to pesticides. 9/87

• Journal article on detection and discrimination of
  chemicals in feed. 6/87

• Report on the uses of oysters and fishes as
  carcinogen assay subjects in laboratory
  screening systems. 9/87

• Report on effects of photosynthesis on update of
  selected chemicals. 1/87

• Report on low cost amphibian test for specific
  cytotoxicity effect. 6/87

• Report describing a mathematical model for
  uptake of chemicals by plants. 6/87

• Report on integrated multiple endpoint test to
  screen for dermal toxicity, neurotoxicity and
  acute toxicity. 8/87

• Report on biochemical and pathological effects of
  contaminated sediment on marine fishes. 9/87

• Journal article describing relationships between
  microbial degradation rate constants and
  chemical properties. 9/86

• Report on efficacy of fish embryo infection assays
  to screen environmental carcinogens. 9/87

• Report on comparison of rapid estuarine/marine
  chronic tests results with those from long-term
  chronic tests with synthetic chemicals. 9/87

• Methods manual for marine algae toxicity tests.
  9/87
Structure Activity Relationships (SAP)

1987 Program Outlook

Health Effects

• Structure-activity relationships based on causally
  derived parameters for one class of chemicals of
  interest to OTS. 9/87

• Pattern recognition analysis of azo dyes using
  mammalian azoreductase. 9/87

• Report on SAP studies on chlorinated pyridines.
  3/87

• Review of approximate methods for calculation of
  molecular electrostatic potentials and their use
  in the comparison of chemicals for toxicological
  assessment. 6/87

• Identification of substructures associated with
  Salmonella mutagenicity by CASE-SAR analysis
  for PMN use. 9/87

• Computer assisted structure-mutagenicity
  relationships for some nitrogen containing
  chemicals of interest to OTS. 3/87

• Provide a combined data  management system for
  genotoxic chemical. 12/86

• Journal article on the genotoxicity of acrylates in
  cultured mammalian cells. 8/87

Environmental  Processes

• Report describing SAR models for predicting the
  toxicity and uptake to terrestrial plants and
  animals.  6/87

• Report on evaluation of SAR methods for
  estimating physical/chemical properties of
  industrial chemicals. 10/86

• Delivery of Version 1 of a computer system for
  QSAR  estimation of physical/chemical
  properties and ecotox. effects. 6/87

• Report on database for QSAR models of
  ecotoxicity. 8/87

• Feasibility report on the development of SAR
  models for chronic toxicity to fish, invertebrate,
  and algae. 9/87
Special Human Data Needs

1987 Program Outlook

• Determine feasibility of using monitoring or
  screening methods to assess exposure-effect
  relative to two organ systems. 1 2/86


Ecology:  Transport,  Fate and Field
           Validation

1987 Program Outlook

Environmental Processes

• Final data report on the field validation of
  freshwater hazard assessment methodology.
  12/86

• Users Guide/Manual for Unsaturated-Saturated
  Zone Pesticide Exposure Model. 1/87
                                               13

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• Report on spatial variability of soil release
  characteristics (Dougherty Plain Site) for use in
  PRZM (testing). 3/87

• Journal article on results of field testing of PRZM
  and PESTANS leaching models for coastal plain
  soils. 3/87

• Report on chemical characteristics of a pesticide in
  aquatic field site  after use application. 4/87

• Journal article: Correlation Between Tissue
  Cholinesterase Levels and Chemical Exposure in
  Avians. 6/87

• Journal article of spatial variability of pesticide
  application. 11/86

• Dougherty Plain Annual Report. 9/87

• First Annual Report—North Carolina Pesticide
  Runoff-Estuarine Exposure/Risk Assessment
  Field Study. 9/87

• Report on mathematical models for transport and
  transformation of toxic chemicals in subsurface
  environments. 10/86

• Report on comparative sensitivity of larval stages
  of pelagic spawning  estuarine fishes to toxic
  substances  12/86

• Project report on  rates, constraints, and kinetic
  formulation for organic  chemicals and metals.
  12/86

• Report on environmental assessment on an azo
  dye. 5/87

• Report on microbial transformation rate constants
  for chemicals of high priority to OTS. 3/87

• Journal article on amphipod crustacean Hyalella
  azteca acute freshwater sediment toxicity tests.
  3/87

• Report on response  of laboratory and field
  communities to stress:  impact of
  pentachlorophenol.  3/87

• Journal article in bioaccumulation of
  hexachlorobenzene  from contaminated
  sediments by amphipods and Oligochaetes. 8/87

• Journal article on freshwater sediment acute
  toxicity test with the amphipod crustacean
  Hyalella azteca. 8/87

• Report relating response of single species and
  benthic communities to toxic chemicals. 8/87
• Report on SARs of chemical toxicant groups as
  measured by microecosystem functional
  responses. 11/86

• Journal article on aerobic biodegradation of
  complex organic mixtures. 1 2/86

• Book chapter—Abiotic Organic Chemical
  Transformations at the Sediment Particle-Water
  Interface. 5/87

• Journal article on oxidation processes in aquatic
  environments. 6/87

• Journal article on environmental factors affecting
  microbial transformation rates of pollutants.
  8/87

• Progress report on acute/chronic toxicity
  extrapolation method for freshwater organisms.
  9/87

• Report on a draft protocol for a naturally derived
  mixed cult microcosm for hazard evaluation of
  toxic chemicals. 9/87
Health: Markers, Dosimetry and
         Extrapolation

1987 Program Outlook

• Report on the application of the parallelogram
  extrapolation approach for pesticides. 9/87

• Report on the effects of formamidines on visual
  evoked potentials. 10/86

• Comparison of In Vitro and In Vivo Skin Absorption
  of DINOSEB in Rats. 9/87

• Report on comparison of tumorigenicity for 5
  mineral fibers by means of intrapleural
  innoculation. 6/87

• Report on the use of in vitro systems to assess the
  carcinogenicity of asbestiform minerals. 9/87

• Validation of a Human Behavioral Test Battery:
  Effects of CO Exposure on Human Performance.
  9/87

• Report on effects of prenatal exposure to selected
  organic compounds on development of sensory
  systems. 6/87

• Report on teratogenic effects of diphenyl ethers  on
  the pulmonary system of neonatal rats. 9/87

• Report on percutaneous absorption of organic
  compounds in young and adult rats. 9/87
                                               14

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 • Onset Visual Evoked Potential and Motion
   Adaptation in Humans: A Study to Provide Data
   for Rat to Man Extrapolation. 6/87

 • Validation of a Learning Paradigon: Effects of
   Selection Delay and Scopolamine and
   Acquisition and Steady-State Performance on an
   Automated Radial-Arm-Maze. 3/87

 • A Novel Method for Detection and
   Characterization of Neuronal Phosphoproteins
   Bound to Nitrocellulose. 9/87

 • Evidence That Genes for Tumor Markers are
   Clustered with Cellular Oncogenes on Human
   Chromosomes. 7/87

 • Report on expression of cellular oncogenes in
   normal and transformed respiratory trace cells
   and implications for risk assessment. 8/87

 • Evaluation of Industrial Chemicals in the Rat
   Tracheal Cell Transformation Assay on Potential
   Human Respiratory Carcinogens. 5/87

 • Report on the use of evoked brain potentials in the
   evaluation of toxic exposures. 10/86
Exposure Monitoring

1987 Program Plans

• Interim report on pollutant sources and human
  exposure to toxic pollutants using TEAM data—
  FY 1987. 12/87

• Users Guide—Data Base Management System
  (CIS) for EMTS. 12/87

• Annual Report of Pesticide Total Exposure. 9/87

Biotechnology/Microbial and
Biochemical Pest Control Agents

1987 Program Plans

Health Effects

• Report on the competition of endogenous and non-
  endogenous intestinal microorganisms: utility in
  health research. 8/87

• Journal article on recombination of DNA in
  plasmids and genomes of bacilli. 9/87

Environmental Processes

• Report on biological control agent testing
  requirements  for representative freshwater
  species. 12/86
 • Report on lab testing and evaluation of selected
   MPCA's on non-target arthropods. (Terrestrial)
   6/87

 • Report on susceptibility of stressed non-target
   species to microbial pest control agents. 9/87

 • Journal article: Evaluation of Techniques for
   Detection and Enumeration of Trans-Conjugates
   in Lab Media. 11/86

 • Journal article: On Methods to Evaluate Conjugal
   DNA Transfer in a Terrestrial Microcosm. 1 2/86
Engineering Release and Controls

1987 Program Outlook

• Evaluations for Polymer Gloves for Agricultural
  Use. 9/87

• Assessment of Toxic Exposures and Releases from
  Filtration Unit Operations: Feasibility Study.
  11/86

• Report on methodologies  for estimating clothing
  performance for PMN review process. 9/87

• Interim report on laboratory testing of full-
  facepiece respirators. 9/87

• Evaluation of plastic barrier effectiveness, glove
  bag removal, and localized vacuum removal.
  9/87

• Evaluation of the effectiveness of O&M guidance
  provided for asbestos abatement. 7/87
Ecology: Ecotoxicity and Risk
          Assessment

1987 Program Outlook

• Initial Design and Specifications of Computerized
  Terrestrial Exposure Model. 9/87

• Soils and Meteorological Databases for Terrestrial
  Exposure Models. 9/87

• Report on intertaxa correlations for toxicity to
  aquatic organisms.  9/87

• Report on baseline data and database definition
  for seagrass ecosystems. 9/87

• Report on efficacy and accuracy of empirical
  models of toxic impacts and species interactions
  in small mammals.  9/87
                                              15

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• Report on prelim, ident. of existing models to
  predict physiologic and life history consequences
  of animal body burdens of xenobiotic chemicals.
  9/87

• Report on wildlife population model dynamics:
  emphasis on identification of critical
  components. 12/86

• Report on development of ecosystems resiliency
  data base. 7/87

• Documentation report on databases to support
  aquatic ecosystem models. 8/87

• Journal  article: Describing Algorithms for Using
  Standard Laboratory Results to Predict Effects on
  Natural  Populations. 8/87

• Prototype Population Model and Software
  Incorporating Lethal Effects of Narcotic
  Chemicals on Natural Populations. 2/87

• Update WASP 4.1 aquatic exposure model for risk
  assessment. 9/87

• Computer program of model predicting kinetic
  exchange of hydrophobic xenobiotics. 9/87

• Report on comparison of  models for representing
  bioconcentration and biomagnification in fishes.
  9/87

• Report on selection of critical terrestrial
  ecosystems. 9/87

• Report documenting the coupled plant-soil model
  and software for predicting the fate of xenobiotic
  chemicals in terrestrial plants. 9/87

•  Documentation report on preliminary models and
   software that predict wildlife body burden of
   xenobiotics as a func. of exp. rte. and avoidance
   mechanism. 9/87

•  Report on efficacy and accuracy of empirical
   models  of plant species interactions;
   development of existing  models to incorporate
   chemical insult. 9/87

•  Report on survey and analysis of mathematical
   models  suitable for assessing risk to terrestrial
   ecosystems from releases of xenobiotics. 9/87

•  Review  article on effects of chemicals on soil
   microorganisms responsible for ecosystem
   processes—carbon, nitrogen, phosphorus, sulfur
   cycles. 9/87
Support

1987 Program Plans

Monitoring

• FY 1986 Annual Report on intercomparison
  program for pesticides. 6/87

• Annual Report: EPA Pesticides and Industrial
  Chemicals Repository. 6/87

• Manual of Quality Assurance in Pesticide
  Laboratories.  1/87

• Revised and updated manual for analytical
  methods for tissue and environmental samples.
  1/87

• Evaluation report on air models to assess human
  exposure to toxic organic compounds. 1 2/87

• Report on asbestos audit program. 12/87

• Annual report—QC sample program EPA
  repository for toxic and hazardous materials—
  FY 1987. 12/87

• Development and Validation Procedures for Total
  Exposure Multi-Media Models (Air Model
  Evaluation). 12/86

• Annual report—QC sample program EPA
  repository for toxic and hazardous materials—
  FY1986. 12/86

Environmental Processes

• Internal report on status of exposure and risk
  modeling support provided by CWQM team.
  9/87

• Development of Potential Risk Identification
  System for  Existing Industrial Chemicals by ITC.
  6/87

• Internal report on status of chemical reviews,
  assessments  and modeling support/training
  provided. 9/87

• Report on the toxicity-persistence of Cationic Poly-
  Electrolytes. 9/87
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Hazardous Waste/Superfund Research
Committee


Introduction
  The Resource Conservation and Recovery Act
(RCRA) authorizes a regulatory program to identify
wastes which pose a substantial hazard to human
health or the environment, and to  develop
management standards for wastes which protect
human health and the environment. Research
support for this program provides the scientific and
engineering bases for characterizing wastes,
determining the hazards they pose, and formulating
controls. In addition, Section 311 of the Clean
Water Act authorizes research to support
prevention and control of hazardous materials
releases.
  The Office of Emergency and Remedial Response
(OERR) and the Office of Waste Programs
Enforcement (OWPE) require scientific and
technical support from the Office of Research and
Development to mitigate health and environmental
problems at priority sites listed under the
Comprehensive Environmental Response,
Compensation and Liability Act (CERCLA). ORD's
program provides a core of scientific and technical
expertise to support the implementation
requirements of CERCLA and the enforcement
actions undertaken to obtain cleanup and recovery
of costs. It concentrates on evaluating equipment
and techniques for discovering, assessing,
preventing, controlling, removing, and ultimately
disposing of hazardous substances released into
the environment. Many of the Superfund-
sponsored activities consist of field testing and
evaluating technologies developed in other
research programs, such as hazardous waste;
however, as a result of the Superfund Amendments
and Reauthorization  Act of 1986, ORD will, for the
first time, begin research that is focused on
problems that are  unique to Superfund sites.
  The major research issues identified by the
Assistant Administrators for the Office of Solid
Waste and Emergency Response (OSWER) and the
Office of Research and Development (ORD) provide
the framework for this discussion  of the ORD
research accomplishments for FY  1986 and the
proposed program outlook for FY 1 987.
ISSUE: Alternative Technologies
  Information and data developed in this issue
support the Office of Solid Waste (OSW)
implementation of those portions of the RCRA
amendments which require banning high-hazard
wastes from land disposal. For safe disposal of
such wastes, the effective alternative or waste-
altering treatment processes must be evaluated
and performance parameters established.

1986 Program Accomplishments
  For evaluation of existing full-scale treatment
technologies which support OSW's "Best
Demonstrated Available Technologies" (BOAT) land
disposal restriction program, performance of
several alternative treatment process units were
evaluated for distillation, thin film evaporation,
steam stripping and biological treatment for
hazardous waste solvents; neutralization, cyanide
destruction, chromium reduction,  and chemical
precipitation for metals, cyanides, and corrosive
wastes. Test data show that these alternative
treatment technologies are preferable to direct land
disposal, and that good design  and operation are
critical for reduction of waste hazard potential to
acceptable levels.
  Other data characterize  residuals at 10
incinerators. Generally, these data show that
incinerator ashes would pass the existing toxicity
test (EP); however, in  some cases, they also show
that the incinerators would not pass the proposed
Toxicity Characteristics Leaching Procedure (TCLP)
for metals and organics.
  Technical Resource documents  on treatment
alternatives for dioxin and solvent wastes were
produced. These TRDs provide  information  on
design and performance of alternative treatment
technologies for treating wastes banned from
direct-land disposal.
  Six pilot-scale units for in-house cyanides and
metals research were fabricated and installed.
Inorganic waste treatment data were generated for
neutralization, lime precipitation, sulfide
precipitation, chromium reduction, cyanide
oxidation, and mixed-media filtration. Two other
pilot-scale units for in-house biological treatment
research were installed.
  Waste-minimization case studies were conducted
on seven processes in support  of the Waste
                                               17

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Minimization Report to Congress. Waste-
minimization audit procedures developed were
applied to three waste-generating facilities. Audit
results were encouraging. Several significant
opportunities for reducing cyanide and solvent
waste generation having paybacks of less than one
year were identified. In FY 1987, this latter program
will include four of the "first third" waste streams
prohibited from land disposal.
  The Hazardous Waste Engineering Research
Laboratory sponsored the annual hazardous waste
research symposium. Approximately 1,000 people
attended and some 60 papers were  presented.
  Significant technical and laboratory testing
support was provided to Environment Canada in a
cooperative project. Protocols for testing solidified
hazardous waste were evaluated. In the area of
emerging technologies, field application of APEG
(alkaline polyethylene glycol) reagents was
successfully demonstrated at Montana Pole, an
inactive wood-preserving site in Butte,  Montana. At
this site, 9000 gallons of pentachlorophenol-
contammated light  oil containing chlorinated
dibenzodioxm and chlorinated dibenzofuran (CDDs/
CDFs) homologs, ranging from 422  ppb of tetra
isomers to 84,000 ppb of octa isomer, had been
separated from ground water  From Butte, the test
equipment was sent to Kent, Washington, where it
was used to treat 8,000 gallons of solvent-oil
wastes containing CDDs and CDFs.
  In laboratory tests, oil from the Montana site was
effectively decontaminated by the APEG reagent at
conditions as  mild as 70°C after 1 5  minutes. Total
TCDDs and total TCDFs were reduced from 422 ppb
and 147 ppb to below detection limits for TCDDs,
and to 33 3 ppb for TCDFs. At  100°C and 30
minutes of reaction time, all of the CDD/CDF
homologs were reduced to quantities below
detection limits. The field equipment used to
implement the chemical  process consisted of a
2700-gallon reactor mounted on a 45-foot trailer
and equipped with  a boiler/cooling  system and a
laboratory/control room.
  The APEG process, which successfully destroys
CDDs and CDFs in liquid haloorganic wastes, may
revolutionize the treatment of such  wastes. The
process cost in the  Butte, Montana
decontamination field demonstration was less than
10 percent of the projected cost of on-site
incineration.
  Related work with KTEG (potassium
tetraethylene glycol) reagent has shown that
complex to be capable of destroying ethylene
dibromide (EDB) and a host of other halogenated
organics. The most recent work with KTEG was
oriented toward reactor design for full-scale
destruction of EDB formulation stocks and
investigation  of the reaction kinetics of KTEG with a
series of compounds. The reagent successfully
dehalogenated carbon tetrachloride, chloroform,
methylene chloride, ethylene dichloride, ethylene
dibromide, and chloropicrin.
  The white-rot fungus (Phanerochaete
chrysosporium) has been found to mineralize a
broad spectrum of persistent organopollutants
including: pentachlorophenol, hexachlorobenzene,
lindane, chlordane,  Mirex, atrazine, DDT, DDE,
benzo(a)pyrene, methoxychlor, 2,3,7,8-TCDD, and
PCB congeners. After 90 days of incubation and
two additions of glucose, more than 99 percent of
the DDT was degraded. This naturally occurring
organism  shows promise for the treatment of toxic
pollutants, even in  low concentrations.

7557 Program Outlook
  During 1987, in cooperation with  the state of
California, the alternative technologies program
will concentrate on evaluation and demonstration
of six alternative treatment processes. Six to eight
existing treatment systems for controlling
hazardous wastes banned from land disposal will
be evaluated. Process evaluation will include
composting of toxic wastes, UV-hydrogen peroxide
treatment of pesticide rinse wastes, treatment of
auto-shop wastes, low temperature thermal
treatment, chlorinated solvent adsorption, and an
HF acid treatment process. Three more pilot-scale
technologies for m-house organic treatabihty
research will be investigated. In-house organic and
inorganic  BOAT treatability studies will support
OSW development  of regulations and respond to
ban-waiver petitions. Testing in FY 1987 will focus
on the "first-third"  listed wastes to  be restricted
from land  disposal under HSWA. Four additional
hazardous waste minimization facility audits will be
performed. Technical resource documents on
alternative treatment technologies for corrosives,
halogenated organics, metals and selected special
waste streams will  be produced. The 1 3th annual
hazardous waste research symposium will be
sponsored. The cooperative study with Environment
Canada on waste solidification will be completed.
Tests to determine  if solidification is an appropriate
means to treat residuals from alternative treatment
processes prior to land disposal will be initiated.
  Work will continue on reactor design and full,
field-scale demonstration of CDD and CDF
destruction in waste oils and on reaction studies for
KTEG detoxification of a series of chlorinated
organic solvents such as chloroform, carbon
tetrachloride, and methylene chloride. Laboratory
testing of  techniques for removing PCBs from
harbor, sewer and lagoon sediments will also
continue.  Currently, in response to needs in Region
II, investigators are  removing and separating the
sediments from water, then using solvent
                                                18

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extraction followed by chemical dehalogenation to
destroy PCBs being entrained/adsorbed by the
solids.
  Research will continue to identify degradation
products of selected organopollutants formed by
the white-rot fungus in simulated waste treatment
systems. This will include a search for the
conditions  necessary to promote mineralization of
the organopollutants and any conditions leading to
inhibition of mineralization. A determination of
lethal concentrations of pollutant will also be
undertaken.
  Full-scale testing of the white-rot fungus
requires knowledge of cultivational practices
specific to the fungus and of requirements for
protection against indigenous microflora. The effect
of selected abiotic soil factors (moisture,
temperature,  pH) on the survival in the absence of
competing  microorganisms of the fungus will be
investigated. To support maximal growth of the
fungus, the ranges of environmental factors will be
optimized. The effect  of soil  biota on the survival
and growth of P. chrysosporium will be assessed to
determine the influence of indigenous microbial
populations.
  Investigation of the complex system of 10-15
extracellular enzymes expressed by P.
chrysosporium will include development of criteria
that can be used to predict the activity of certain
hgnin-degradmg enzymes towards persistent toxic
aromatic pollutants. This  information is critical to
any effort to optimize the  degrading ability of P.
chrysosporium towards these persistent pollutants.

ISSUE: Waste Characterization
  Human health, environmental effects and risk
assessment information and procedures are
developed to assist in the characterization of waste
and assessment of their hazards. This information
will assist m the development and revision of
regulations, and will be used in permitting and
enforcement decision-making. Research products
will provide simpler, less costly, and more accurate
information and risk assessment methodologies.

1986 Program Accomplishments
  During FY 1986,  62 Health and Environmental
Effects Profiles (HEEPs) were prepared for use by
the Office of Solid Waste  in  making listing
decisions under Section 3001 of RCRA. To support
the Hazardous and Solid Waste Amendments—
1 984 requirements which restrict land-disposal  of
certain hazardous wastes, 100 Reference Doses
(RfDs) and 40 carcinogenicity evaluations were
prepared. As RfDs are prepared and verified, they
are entered into the Integrated Risk Information
System (IRIS). Currently, 90  such profiles are in
IRIS. Four petitions or Exposure Information
 Reports were reviewed and assistance on health/
 exposure assessment provided to work groups and
 the permit assistance team. Forty-five subchronic
 testing protocols were developed for specific
 chemicals, and work continued on various aspects
 of health and exposure assessment methodology.
   Under development are short-term in vivo and in
 vitro bioassays for use in screening protocols to
 determine whether wastes, as single chemicals or
 complex mixtures, are hazardous. The screening
 protocol for determining potential adverse effects
 on human health included cancer and non-cancer
 endpoints. Toxic endpoints evaluated included
 carcinogenicity/mutagenicity, neurotoxicity,
 reproductive effects,  teratogenicity, general toxicity
 and immunotoxicity.  The findings with four known
 toxic chemicals, each with specific toxic endpoints,
 gave very good predictability for each specific
 endpomt. Validation studies indicated that
 screening is capable  of detecting specific biological
 activity of pure compounds as single components.
 Since most hazardous waste and hazardous waste
 sites contain complex mixtures, future screening
 will focus on more single-chemical constituents of
 complex mixtures, including comparative organ-
 specific toxicity, interactions, synergism, and
 antagonism.
   Activities in  environmental processes research
 supported risk assessment, listing, and waste-
 banning decisions. The behavior of two groups of
 important ground-water contaminants,
 alkylbenzenes and halogenated aliphatic
 hydrocarbons, was studied in microcosms
 containing actively methanogenic aquifer material.
 Although chloroalkenes degrade anaerobically to
 produce products that are more  hazardous and
 more mobile, this study showed that sequential
 reductive dechlormation need not result in the
 appreciable accumulation of products and that the
 reduction can be rapid and extensive once activity
 begins. The significant anaerobic degradation of the
 alkylbenzenes has important implications for
 remediating polluted  ground waters.
  An in vivo fish model was adapted to monitor
 respiratory-cardiovascular responses of rainbow
trout exposed to acutely toxic aqueous
concentrations of chemicals. Observed responses
for acute toxicity syndromes in the fish have
allowed the grouping of similar response sets
caused by other chemicals into four types: AChE
inhibitors, respiratory irritants, respiratory
uncouplers, and narcotics.
  A metal speciation  modeling approach was
developed for evaluating potential mobilities of
arsenic, barium, cadmium, chromium, lead,
mercury, nickel, selenium,  silver, and thallium in
ground waters under conditions reflecting leachate
contamination from a failed land disposal facility.
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Although these studies divide the metals into
"mobile" and "relatively immobile" groups, a high
degree of uncertainty still exists about such
predictions because of the wide variability in
ground-water characteristics, and the lack of redox
equilibria in many ground-water systems.
Information was gathered on the role of
microorganisms in the anaerobic transformation of
xenobiotic compounds for assessment of kinetic
concepts of degradation in natural environments.
Finally, to provide input to the mathematical model
developed to estimate potential ground-water
contamination from  chemicals in land disposal
sites, extensive laboratory measurement of
hydrolysis rate constants for 26 compounds
regulated under RCRA were performed.


7557 Program Outlook
  Approximately 90  HEEPs and 180 pre-HEEPs will
be prepared for OSW use in FY  1 987. In addition,
100 Reference Doses and 40 carcinogenicity
profiles will be prepared. Additional health and
exposure evaluations will be provided in support of
ban-waiver petitions, ACL and permit applications.
Major new  emphasis will be on development of risk
assessment methodology, including the initiation of
a project to develop guidance on risk assessments
for the incineration process.
  The health research program  will continue
development of a screening protocol for
determining waste toxicity using short-term in vivo
and in vitro bioassays. This protocol is used for
predicting potential human health hazards and as
an indicator of future research needs. Bioassays for
the most significant, potentially debilitating, health
endpoints are under development to reduce the
uncertainties in assessing hazards and  risks from
complex mixtures of unidentified and
uncharacterized wastes.
  Environmental processes research will be
conducted in three major areas: risk assessment,
listing, and land disposal restriction programs.
Multimedia assessment models for predicting
potential migration of wastes from land disposal
sites into the air, soil and ground water will be
developed as will quantitative structure-activity
predictions of waste toxicity and models for
predicting waste concentration  in ground water.
Studies  have been initiated to determine the ability
of wetlands to retain or transform hazardous
wastes.

ISSUE: Dioxin
  This research supports Agency assessments of
the extent and severity of dioxin contamination and
development of control procedures. Research
continues in health and risk assessment activities
and in transport and fate and quality assurance
assistance. Technologies with the potential to
detoxify or decontaminate dioxins and dioxm-like
compounds are also being evaluated.

1986 Program Accomplishments
  Engineering evaluated chemical reagents,
alkaline polyethylene glycol (APEG) reagents, and
use of the mobile incinerator for treatment of dioxin
contaminated soils. Methods for decontaminating
PCB transformer and capacitor fire sites were also
evaluated.
  In laboratory soil column and batch leaching
experiments using contaminated soils from
Missouri and New Jersey, TCDD was shown to be
strongly bound to the waste-soil matrix. Disposal of
these soils by themselves would be safe as regards
leaching and mobility of TCDD in water. A report
"Solubility of 2,3,7,8-TCDD in Contaminated  Soils"
indicated that solubility of TCDD in these soils was
regulated not by clay and native organic matter, but
by the levels of other organic  contaminants present
in the wastes with the  TCDD. This finding suggests
that soils containing higher concentrations of other
organic contaminants would allow leaching of
greater amounts of dioxins.
  During FY 1986, an analysis of soil ingestion
rates was performed to assist in the determination
of dioxin exposure to humans from ingestion  of
contaminated soil. From a literature review, the
vapor-phase photolysis of 2,3,7,8-TCDD and the
kinetic and tissue distributions of 2,3,7,8-TCDD
were evaluated.
  Monitoring research produced an  evaluation
report on current methods for analyzing 2,3,7,8-
TCDD. Reference standards were prepared for tetra
isomers and for the important isomers in the  penta,
hexa and hepta homolog series. Experiments were
conducted to evaluate co-elution and to optimize
analytical procedures for identification and
quantitation in environmental samples. A
monoclonal  antibody to 2,3,7,8-TCDD was
produced and will be evaluated for its ability to
detect 2,3,7,8-TCDD in samples.
  Of particular note is  the major progress achieved
in the development of a monoclonal assay for TCDD
in the health program. A thyroglobulin was
conjugated with dioxin and this conjugate was used
to immunize mice and  produce hybridomas. To
assay the cultures, a solid-phase
radioimmunoassay was developed. Fourteen
hybridomas  were identified that produce
monoclonal  antibodies reacting with bovine serum
albumin-TCDD (BSA-TCDD) but not  with BSA
alone. Two hybridomas showed preferential binding
of BSA-TCDD of more than 200-fold. One of the
two was purified and showed high binding to BSA-
TCDD; this should allow detection of less than  1 ng
of dioxin per ml or about 50 pg in  a 50 p\ sample. In
                                                20

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general, the procedure looks promising and further
attempts will be made to demonstrate specificity
and binding characteristics.
  Also determined was the linear relationship
between in vivo toxicity of dioxin (weight loss,
thymic atrophy) and induction of hepatic
microsomal cytochrome P-488-dependent
monooxygenases AHH (aryl hydrocarbon
hydroxylase) and EROD (ethoxyresorufin O-
diethylase). More importantly, there was excellent
quantitative correlation between the in vivo
structure activity relationships for 15 PCDFs and
their in vitro activities such as AHH induction in rat
hepatoma H-r-ll E cells and as ligands for the
2,3,7,8-TCDD receptor protein. This makes the use
of the in vitro AHH induction assays suitable as a
short-term quantitative test system for this class of
compounds.
  Work continues to define the cytosolic receptor
binding as an assay applicable as a short-term test
system for PCDDs and PCDFs using cell cultures.
Results to date are promising and the  study has
progressed to the use of radiolabeled ligands of
TCDD and TCDF for determination of the binding
power and further characterization of the receptor.
Work is also progressing to develop and test
computational structure-activity relationship
methods based on molecular properties related to
the mechanisms of interaction with biological
systems. The work is  also addressing ways to
assess the toxicity of  dioxin-like chemicals.
  Laboratory experiments were conducted to
determine the bioavailability of polychlorinated
dibenzo-p-dioxins in the aquatic environment to
two species of fish—carp and fathead  minnows.
Although the nominal water concentration of
2,3,7,8-TCDD was 0.2 ppt, based on input of stock
solution, a large portion of the material was
associated with suspended solids or was attached
to the aquaria walls. The "dissolved" 2,3,7,8-TCDD
concentrations were only about 0.005 ppt or 2.5%
of the nominal water  concentration due to TCDD's
extreme hydrophobicity. Although the  rate of
uptake of 2,3,7,8-TCDD appears to be  slow, the rate
of depuration is even  slower, and the steady-state
bioconcentration factor (BCF) for fathead minnows
is estimated to exceed 80,000 and for  carp 33,000.
BCFs calculated on the basis of the dioxin
concentration in water free of suspended
paniculate matter are approximately six times
greater or in excess of 200,000 for carp. Although
no-effect water concentration cannot be predicted;
for carp, it would probably be less than 1 ppq.

1987 Program Outlook
  The health research program will develop test
methods to detect health effects and will collect
data  relevant to the assessment of human health
effects from dioxin and related compounds.
Endpoints to be studied include neurological effects
and immunotoxicological effects. This research will
further the development of a method for using
molecular designators to assess and predict
toxicological activity of dioxin-like compounds.
  Engineering research will address mobility of
dioxins from soils containing high levels of other
organic contaminants. High priority is being given
to soils from wood preservative sites that are
contaminated with oil/pentachlorophenol and
higher chlorinated dioxins and furans. Data from
this work will be used by OSW in listing decisions
under RCRA and in guidance for making site
specific decisions on "how clean is clean."
  Environmental processes research will continue
work to determine rates of uptake, body burden and
tissue distribution of 2,3,7,8-TCDD through
exposure to fish, plants, and large mammals. In
addition, the mobility of dioxins in soils and the
degradation of 2,3,7,8-TCDD and other dioxin
isomers will be evaluated, as will the rates of
photolysis of 2,3,7,8-TCDD and other dioxin
isomers.

ISSUE:  Waste Identification
  Analytical methods development for identifying
hazardous wastes and their chemical constituents
will continue. Analytical methods for enforcing
Section 3001 of RCRA must be standardized and
tested to  determine their validity and reliability.
New methods and procedures for detecting the
presence of hazardous wastes under field
conditions are also required to satisfy RCRA facility
monitoring requirements.

1986 Program Accomplishments
  1986 accomplishments included work on the
development, evaluation and validation of
monitoring methods. Generic methods for analysis
of Appendix VIII compounds were developed. The
generic approach being developed is intended to
reduce the  cost and time needed for the analysis of
wastes. One example is the evaluation of a general
extraction procedure, EP III, for hazardous  waste.
Another is a thermal-spray coupling device to make
solid and nonvolatile analysis possible by MS/MS
instrumentation.
  To assist with problems associated with
measuring  an ever-increasing number of organic
and inorganic contaminants in complex
environmental matrices, at ever-decreasing levels
of sensitivity, monitoring research has worked on
the development of advanced analytical methods
for detection, including such techniques as Liquid/
Gas Chromatography, and Mass, Fourier Transform
Infra-Red, and Inductively Coupled Plasma
Spectroscopy. Also, biological procedures have
                                               27

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been evaluated as possible analytical screening
techniques.
  Several monitoring methods designed to map
subsurface conditions were evaluated. These
methods included electromagnetic conductivity,
ground penetrating radar, and resistivity. The
standardization of subsurface monitoring methods
was initiated in order to improve the quality of data
collected.

1987 Program Outlook
  Several projects will be pursued at the Ada and
Las Vegas laboratories to improve ability to monitor
pollutants in surface soils, the subsurface
unsaturated zone, and ground water. These
include  geophysical techniques to characterize site
geohydrology and locate  surface contamination;
among the FY 1 987 projects are  investigations of
the capabilities of electromagnetic induction,
resistivity, and magnetometry techniques and their
subsequent use in EPA field operations; coupling of
lasers and downhole fiber optics for direct
measurement of groundwater quality; and
evaluation of other traditional ground-water
monitoring methods. Research on subtitle D
facilities will include development of expert
systems for location standards and monitoring in
wet environments and fractured  zones.
  A computerized Geographical Information
System (CIS) for integrating terrain, remote sensing
and sampling data will be evaluated and made
available for pilot use in the Regions. The Las
Vegas laboratory will continue to provide overhead
remote sensing to locate waste disposal sites,
detect waste discharges, identify erosion and other
types of deterioration, and to define environmental
impacts of land use.

ISSUE:  Land Disposal
  Research in  land disposal provides guidance on
design, permitting, operation, maintenance, closure
and regulation of land treatment, storage and
disposal. Other research subjects include large-
volume waste disposal, mining wastes, solid waste
(non-hazardous) characterization and technology
transfer. In addition, source characterization and
control techniques for air emissions from
hazardous waste treatment, storage and disposal
facilities are the focus of research activity.

1986 Program Accomplishments
  During FY  1 986, several major accomplishments
were achieved in support of the Office of Solid
Waste (OSW).  A report on prediction and mitigation
of subsidence damage to covers  of hazardous
waste landfills was completed. The final report
presents methods to estimate the amount of cover
subsidence that may be expected when the
physical characteristics of wastes are known.
Settlement time may also be estimated. Methods
are described to prevent or mitigate subsidence.
Included are methods to hasten pre-closure
settlement and methods to strengthen waste
materials to minimize the amount of post-closure
settlement. Containerized (e.g., drummed) wastes
were found to contribute most to post-closure
settlement, and thus to subsidence in a  RCRA-
permitted  landfill. This contribution may be
prevented by  not landfilling with contained waste
and by filling  all voids with high strength materials.
The final report was prepared in response to a
critical OSW  need for information on the potential
for subsidence damage to hazardous waste landfill
covers.
  A project was undertaken to characterize
municipal  waste combustor residue and its
leachate. The information gained will contribute
directly to  a critical need of the Office of Solid
Waste for  meeting a Congressional mandate in the
Hazardous and Solid Waste Amendments (HSWA)
of 1 984. That mandate requires a report to
Congress as the basis for further needed action
regarding the possible regulation of the residues as
hazardous waste.  The project will sample bottom
ash, fly ash, quench water, and ash landfill
leachate for comprehensive analysis of hazardous
components.  Based upon earlier results from other
investigations, unacceptable concentrations of
heavy metals  and  several complex organics may be
present. This  project will continue in FY 1 987.
  A technical  guidance document was issued.
Entitled, "Construction Quality Assurance for
Hazardous Waste  Land Disposal Facilities," this
report discusses the five elements of a construction
quality assurance  (CQA) plan (responsibility and
authority, CQA personnel qualifications, inspection
activities, sampling strategies, and documentation).
The document also addresses inspection activities
for six facility  components. These components are
foundations, dikes, low-permeability soil liners,
flexible membrane liners,  leachate collection
systems, and  final cover systems. This guidance
document  will help CQA personnel to ensure that a
constructed hazardous waste land disposal facility
will meet or exceed all design criteria, plans and
specifications.
  Two reports on leachate collection systems for
hazardous waste facilities were completed. The
first, "Leachate Collection and Gas Migration and
Emission Problems at Landfills and Surface
Impoundments," identifies and describes potential
problems and is based on  interviews with
regulatory officials, design engineers, and
commercial firms  involved  in hazardous waste land
disposal facility management. The second,
"Avoiding  Failure  of Leachate Collection and Cap
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Drainage Systems," describes state-of-the-art
design, construction, inspection, maintenance, and
repair activities to avoid system failures.
  The report, "Estimating Leachate Production
from a Closed Hazardous Waste Landfill," was
issued. It presents a decision-free-based approach
to estimating leachate production rates and times,
and provides several practical examples of the
approach in estimating production in both
hypothetical and real-world situations.
  Significant technical and laboratory testing
support were provided to Environment Canada in a
cooperative effort to evaluate protocols for testing
solidified hazardous waste. Results of this study
will be used by Canada to assist in development of
regulations for solidification/stabilization
technology. The results will provide that agency
with critical data on the applicability of regulatory
leaching procedures to predict performance of
solidified/stabilized waste.
  Two major laboratory studies demonstrated that,
in contrast to the effects of pure solvents, diluted
solvents or very low concentrations of  chemical
compounds in landfill leachates have no significant
effect on clay soil permeability over the term of
laboratory measurements. The data obtained from
studying a variety of methods for testing permeant
liquid, soil type, and permeability confirm that,
where permeability is used as a measure of
leachate/liner compatibility, site-specific tests
must be conducted, and the procedure must be
documented in detail if permit reviewers are to fully
understand the meaning of the data.
  Data from one and one-half years of hydrologic
monitoring of three-layer cover plot studies have
confirmed the utility of the HELP model in
designing landfill cover systems. Calibration of the
model using short-term observations, however,
may lead to larger predictive errors, emphasizing
the need to consider both short climatic events and
long-term trends in applying the predictive results
to multilayer cover design. This work also
demonstrated the effectiveness of various soil
moisture sensing systems in multilayered soils
placed in accordance with engineering design.
  A study evaluating 10 rapid testing methods for
characterizing different commercially available
bentonite clay types was completed. Using selected
petroleum industry bentonite test methods, specific
clay hydration techniques, and standardized
laboratory techniques, made it possible to
differentiate amounts of unaltered, polymer-
treated, or chemically treated commercial bentonite
products. Correlation between indicator test data
and hydraulic conductivity performance could not
be shown. The data obtained will be useful for
identifying type-distinction of unknown bentonite
products, and for confirming that bentonite
products meet design engineering specifications.
  Development was initiated on four expert
systems to aid in review of RCRA permits for land
disposal sites. They will provide data on the
likelihood of subsidence, chemical resistance of
flexible membrane liners, acceptability of waste
analysis plans, and suitability of surface
impoundment design. The first two of the four
items are ready for testing and the other two will be
ready during FY 1987.
  In FY 1987, twenty-four staff professionals
responded to over 200 requests for technical
assistance from EPA program offices. Regional
offices, industry, state officials, academia, Federal
agencies, and Canada. State-of-the-art technology
seminars were held in all 10 regions and at
headquarters.
  Research was directed toward determining the
feasibility of land treatment of  hazardous waste.
Activities included laboratory, bench- and pilot-
scale studies on soil and waste processes, organics
degradation, and loading  rates. Studies measured
and interpreted the effect of surface soil changes
on the underlying soil and the quality of runoff
water from petroleum landfarms undergoing
simulated closure. The field results over two years
showed insignificant downward migration of the
organics and heavy metals,  and demonstrated that
the closure period of two years in this study
allowed significant reductions  in waste/soil
concentrations. In other work,  laboratory column
and field lysimeter studies were conducted to
evaluate the efficiency of soil core and soil-pore
water samples to detect the migration of the
organic components of long-treated wastes through
soil. Finally, four specific  industrial wastes were
used  in studies to assess the potential for
treatment using soil as the treatment medium.
These wastes were API separator sludge, slop oil
emulsion solids, pentachlorophenol wood
preserving wastes, and creosote wood preserving
wastes. The data, recommendations, and
conclusions are useful for permitting and
regulating land treatment facilities and for their
design and operation.

1987 Program  Outlook
  During FY 1987, technical assistance to program
offices will be expanded to assist in the
development of regulations  and guidance to the
Regional offices in the review of permits and
special problems, to the Office of Enforcement in
developing compliance actions, and to the public in
their waste management activities to protect the
public health and environment.
  The four expert systems initiated during FY 1986
will be tested by selected permit reviewers,
independent subject specific specialists, and
program  office staff. Development of additional
systems will be initiated to assist in other high
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priority RCRA permit-review decision areas.
Specific topics will be selected after further
consultation with the program office staff and
permit reviewers. Anticipated decision areas to be
addressed by the new systems are closure plans,
cover systems, and leachate collection systems.
  A methodology for estimating the reliability of
flexible membrane liners (FML) in field service will
be prepared. The product will be a personal-
computer-based system with which a permit
reviewer or disposal-site operator may determine
the probability of FML failure versus time. The
methodology will be based upon current knowledge
regarding FML characteristics and responses to
given conditions. The conditions will include those
under the user's control (e.g.,  FML thickness) and
those that are uncontrollable (e.g., weather).
  A report will be produced describing the
characteristics of municipal-waste combustor
residues for several sites. The chemical
characteristics of bottom ash, fly ash, quench
water, and ash landfill leachate will be described.
In addition, reports will be provided to OSW to
assist them in their regulatory determination for
large volume wastes. These include the topics of
mitigation techniques for copper heap and dump
leaching, gold/silver heap leaching and conceptual
management practices to control cyanide releases,
and utilization of soils to mitigate cyanide releases
from mining operations.
  A data base on leachate generation
characteristics for municipal solid waste landfill
leachates is being developed and should be
available for use. The data base currently consists
of mostly small-scale research lysimeters, that will
have the capability of receiving and storing data
from full-scale MSW facilities also. A report on
municipal landfill gas condensate will be  prepared
which will evaluate the gas condensate for
hazardous constituents and characteristics. Air
emissions measurement data from the stabilizing
and curing of hazardous wastes will be presented
in another report.
  Preparation of a guidance document for the
design, construction, operation, and closure of an
RCRA surface impoundment will be completed.
Data will be provided on detailed characterization of
leachate from actual hazardous waste facilities.
This  information will assist in liner compatibility
determinations, correlation of liner performance
versus waste, and determining the feasibility of
formulating synthetic leachate for use in  regulatory
tasks.
  ORD will continue to assist the program office in
development, review, and implementation of
regulatory systems for RCRA Subtitle D criteria. A
study of the permeability of a soil liner test section
constructed with normal field  construction methods
and evaluated using both infiltration and  drainage
collection techniques will be completed. Data
already obtained demonstrate the large variability
obtained over an area of field construction.
  The Geotechnical Analysis for Review of Dike
Safety (CARDS) personal computer program will be
completed and distributed to Regional permit
writers and to the engineering community. The
CARDS program provides comprehensive and  rapid
evaluation of dike safety for hazardous waste
surface impoundments.
  Research will attempt to determine the
environmental conditions related to land treatment
site closure. Such environmental protection
parameters need to be identified to permit the
orderly phase-out of land treatment operations.

ISSUE: Incineration
  Technical  information and data are needed to
support permitting of incinerators and improvement
of design requirements.  Results of this research are
used by the Agency and permitting officials to
evaluate the acceptability of incinerating  particular
wastes and in monitoring operating units for
compliance with performance requirements.
Laboratory, pilot and full-scale units are
investigated to determine the  performance of a
range of incinerators and other thermal treatment
devices.
  ORD has generated a large quantity of  existing
environmental performance data from more than
20 EPA-supported incinerator evaluations covering
all of the commonly employed engineering designs
operated by the user community, including liquid
injection, rotary kiln plus afterburner, at-sea types,
hearth or controlled air configurations, etc., and
from several advanced or less popular schemes
such as fluidized bed, molten salt and plasma arc
concepts, etc. Several reports on this broad base of
data have provided OSW, OTS, and OW program
offices the scientific and technical bases  for
developing and promulgating their respective
regulatory approaches and standards for  controlling
the thermal decomposition of  hazardous wastes.
Subsequent industry-supported trial-burn data from
Regional permit actions continue to supplement
and confirm the ORD data base.
  Additionally, ORD's field- and pilot-scale
evaluations of high-temperature industrial
processes including boilers, cement, lime,
aggregate, and asphalt  kilns, and iron-making  blast
furnace concepts represent another 20 facilities of
various designs where hazardous wastes are used
for their fuel value in lieu of fossil  energy sources.
OSW uses this performance information  in their
continuing formulation of regulatory policy for
these methods of treating hazardous waste. These
non-incinerator systems now dispose of a volume
of material by a factor of two or three times  more
than do conventional hazardous waste incinerators.
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  As the need for incineration and other forms of
thermal destruction increases in response to land
bans and Superfund actions, still more information
and detailed performance data must be gathered in
response to a wide spectrum of new questions
posed by the Science Advisory Board as well as the
concerned  public. This additional data is necessary
in order to  effectively support facility permitting
priorities by program and Regional offices, and
involves such issues as improved design and
operating conditions, reliability, monitoring
techniques, residue and total effluent qualities,
delisting, incinerating as-yet untested Superfund
wastes, and more recently the issue of measuring
the biological health impacts from incinerator stack
emissions as compared to those from traditional
fossil fuel combustion. ORD is addressing these
new needs through a continuation of laboratory,
pilot-scale, and field performance evaluations of a
range of incinerators and other thermal treatment
devices.

1986 Program Accomplishments
  In Fiscal Year 1986, the research  emphasized
generating  additional incineration performance
data for dioxm, Superfund and RCRA permit
support actions. Support was provided to the Office
of Water's long-term research needs for ocean
incineration tests on PCBs and organochlorine
wastes; delivering and interpreting ORD's
performance results reports on high temperature
industrial process tests to OSW through
participation in their regulatory policy work group
workshops; mobile monitoring van development
with field testing; continued sampling and analysis
methodology refinements for untested compounds
and real-time monitoring concepts,  and an
extensive amount of field technology transfer and
technical assistance which includes participation in
a permitting program for disposing of Department
of the Army chemical agent inventories.
  Continuing progress was made on the primary
concerns which repeatedly impair the Agency's
ability to permit incinerators, namely products of
incomplete combustion (PICs), toxic  heavy metal
fate and partitioning during thermal treatment,
failure  mode or off-design performance, and  the
new concern of biological health impacts of stack
emissions.  On the latter  issue, two series of  marine
animal toxicity tests were completed at the
Combustion Research Facility (CRF). The CRF
accomplished a PCB trial-burn series in the rotary
kiln, tests of a Superfund site waste involving
solvent contaminated soils, and completion of a
Superfund site dioxin waste test burn report. The
overall  FY 1986 program encompassed various
incineration tests using rotary kilns, liquid injection
units, plasma  arc, industrial boilers, asphalt plants.
Center Hill's turbulent flame reactor, the kiln
simulator burning plastics and chlorinated
compounds, and additional progress in process
control measurement and instrumentation.


1987 Program Outlook
  In 1 987, studies will be conducted to address
priority issues in incineration of hazardous waste
including the potential for increased emissions to
the environment during failure mode or off-design
operation, products of incomplete combustion
(PICs), toxic heavy metals partitioning  and
emissions, and in-depth studies of process
monitoring parameters.
  A field-scale evaluation of incinerator compliance
assessment techniques and performance will
determine the characteristics of all possible
effluents (total mass emissions) in any form (e.g.,
solid,  liquid, gas) at all exit points of a  typical full-
scale  operating incinerator. Research  in the boiler
and industrial furnace areas will continue with
pilot-scale boiler testing at two boilers to study
further PIC and metals emissions under both steady
and non-steady-state conditions in further support
of OSW's boiler regulations.
  A computerized data base will be further
developed to manage both research and field data
on hazardous waste incineration and treatment
processes. The key information in the  data base
includes process design and operating conditions,
waste characteristics, trial burn performance data
and air pollution  control device performance.
  In addition, ORD has been working with OSW to
develop necessary new and expanded incineration
program initiatives and goals to be considered for
FY 1 987 through 1 990 and beyond. These
programs, if funded, will adequately address
current unmet needs for additional research
information related to OSW's urgent permitting and
public information issues. The ORD incineration
research  facilities have been identified by OSW as
being the most useful permitted facility for
conducting pilot-scale evaluations relating to their
initiatives.
  Major classes of incinerators will be evaluated in
both field and pilot/laboratory situations. The
health effects of  the initial waste will  be compared
directly to effects of air emissions and residues to
evaluate whether the chemical alterations resulting
from combustion of the waste represent an
acceptable mitigation of health risk. Health
endpoints to be evaluated include: genotoxicity,
carcinogenicity, pulmonary toxicology, and other
major target organ effects as appropriate. Currently
available toxicological methods will be applied.
  Existing methods will be evaluated for assessing
risks from municipal and hazardous waste
incinerators.  From these evaluations,  interim
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guidance on performing risk assessments from
such incinerators will be developed.

ISSUE: Quality Assurance
  Improved method applications are needed to
assure the reliability of monitoring data used in
support of the RCRA program. The purpose of this
program is to assure that EPA consistently
produces scientifically and legally defensible
environmental data in support of its critical
regulatory and enforcement actions and research
objectives. Analytical standards and reference
materials are developed for and distributed to all
participating laboratories. Quality control and
performance evaluation samples are also being
developed and distributed to appropriate
laboratories. Additional support is provided to State
laboratories to facilitate their performance of the
required measurement and monitoring functions.
This support includes instrument calibration and
provision of reference materials.

1986 Program Accomplishments
  Overall, 30,000 quality control check samples
were distributed to Agency contractors, State, local
and EPA laboratories. More than 20,000 organic
calibration standards were distributed to this same
set of laboratories. All contractor laboratories were
evaluated.

7557 Program Outlook
  Calibration standards and performance
evaluation samples will continue to be developed
and distributed to Agency laboratories, Agency
contractors, state and local laboratories to assist m
standardizing monitoring methods and for
calibration  of analytical techniques used in RCRA
monitoring. Dynamic validation reports will be
completed on all contractor laboratory program
methods.

ISSUE: Control of Hazardous Releases
  Procedures needed to prevent, contain and clean
up accidental discharges of hazardous materials
are the focus of research in this issue. Accidental
releases of oil and hazardous materials occur
frequently and constitute a significant
environmental hazard. Such emergencies include
releases from transportation accidents (rail cars,
tank trucks, vessels, and pipelines); m-plant
releases, where the hazardous material leaves the
boundary of the plant; releases from  uncontrolled
hazardous  waste disposal sites, and releases from
underground storage tanks. The research
conducted  in this program supports the mandates
of Section 311 of the Clean Water Act for release
provisions and Title  I of RCRA for underground
storage tank provisions. The object of this research
program is to develop new and improved
technology for the prevention and control of these
releases and to provide technical information and
guidance regulations, development and
implementation.


1986 Program Accomplishments
  The engineering program completed two
handbooks on hazardous substance release
prevention and removal ("Manual for Preventing
Spills of Hazardous Substances at Fixed Facilities"
and "Reference Manual of Countermeasures for
Hazardous Substance Releases"). The prevention
manual provides guidance on preventing spills of
hazardous substances in fixed facilities that
produce, store, and transport substances from raw
or starter materials. In this manual, emphasis is on
smaller chemical manufacturing facilities and
addresses almost 700 hazardous substances. The
removal manual contains procedures for selecting
treatment and disposal processes, or
countermeasures. Again, some 700 hazardous
substances are addressed. These documents are
providing federal, state, local government, and
industrial personnel with the most up to date
information on preventing and cleaning up
accidental releases of hazardous materials.
  A state-of-the-art report on leak detection
methods ("Underground Tank Leak Detection
Methods: A State-of-the-Art Review") was
published. This report contains a catalog of known
leak detection methods, a description of in-tank
detection methods (as contrasted with inventory or
leak effects monitoring methods), a description of
physical environmental and operational variables
that each method must deal with to obtain a valid
determination, and an evaluation of how well each
method addresses each variable. The significance
to EPA's Office of Underground Storage Tanks (and
to the user community) is that none of the
commercial methods identified appeared to address
all variables adequately. This finding underscores
the importance of the ongoing evaluation of
volumetric leak detection methods,  under which a
full-scale, controlled condition, environmentally
safe test apparatus was designed and installed. The
results of this evaluation will provide direct input
into the OUST development of regulations under
the 1 984 RCRA Subtitle I amendments, and will be
of interest to state and local regulators as well as to
the user community.  Drafts of guidance documents
on preventing leaks from underground storage
tanks (USTs) and on corrective actions to clean up
leaks from USTs were delivered.
  Ten spill prevention control and countermeasure
(SPCC) studies and 1 5 river contingency studies in
support of Section 311 (k) of the Clean Water Act
were completed. Development of a  subsurface
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system for monitoring leaks from underground
storage tanks was initiated for detection of
contaminants away from the leaking tank.
  A state-of-the-art report was completed on
subsurface biodegradation. Although prepared for
underground storage tanks, this report may also
serve as a general state-of-the-art on in-situ
biorestoration of contaminated ground water. The
report indicates that in-situ biodegradation, where
applicable, is potentially a very cost-effective and
environmentally acceptable remediation
technology. Many contaminants in solution in
ground water, and vapors m the unsaturated zone,
can be completely degraded or  transformed into
new compounds by  naturally occurring, indigenous
microbial populations. In other  studies, a  bioassay
protocol employing aquatic and terrestrial
organisms was used to evaluate various solid
waste and industrial leachates for their potential
environmental impacts. A battery of bioassay tests
was also verified in  field trials to determine the
impact of toxic waste sites in actual drainage
systems.
major task will be initiated to develop new and
improved design, construction, and operating
practices for effective prevention of releases from
underground storage tanks and to survey corrective
action techniques for releases from underground
storage tanks.
  Monitoring will continue to provide aerial
photography to assist in the assessment and
mitigation of spills from facilities engaged m
production, storage, processing, and distribution of
hazardous materials. Performance criteria for
evaluating leak monitoring methods will be
established and methods for detecting leaks from
underground storage tanks will continue to be
evaluated. Field measurements will be conducted
and private, local and state leak detection
experiences will be investigated.
  Development of a multi-media bioassessment
screening protocol will continue, with modifications
incorporated to reflect the results of field
evaluations. In-situ cleanup procedures for
controlling leaks from underground storage tanks
will continue to be studied.
7357 Program Outlook
  A guidance document on the use of
nondestructive techniques for locating buried
chemical containers will be published. Of the 17
techniques considered, four were selected as the
most applicable: electromagnetic induction, metal
detection, magnetometer, and ground penetrating
radar. The report will present techniques for
detecting steel and/or plastic containers under
different subsurface conditions.
  A new laboratory method for determining the
effectiveness of chemical  dispersants for
controlling floating hazardous materials will be
issued. This  method is required by regulation and
incorporates scientific advances made since the
original  EPA test was published in 1 971. A
recommended database covering the test results
for numerous combinations  of commonly
transported crude oils  and dispersants will be
available to the Regional Response Teams. The
field test and database should allow RRTs to reduce
the time needed to approve the use of dispersants
at a spill.
  In a cooperative effort with the U.S. Coast Guard,
the Minerals Management Service, and
Environment Canada, a recommended method for
testing offshore oil spill cleanup equipment will be
completed. A major multi-agency  international at-
sea demonstration is scheduled off the  Canadian,
New Foundland coast.
  The evaluation of existing volumetric methods for
detecting leaks from petroleum tanks will be
completed, and a similar effort initiated for tanks
containing chemicals and hazardous wastes. A
Superfund

ISSUE: Site Assessment
  Superfund develops technologies and
information needed for assessing and managing
uncontrolled sites. The success of removal and
remedial actions frequently depends on an accurate
appraisal of the nature and severity of the problem.
Research activities supporting this objective provide
the techniques and procedures for on-scene
coordinators' need to quickly and effectively assess
the degree of hazard and the cleanup requirements
at specific uncontrolled waste sites.

1986 Program Accomplishments
  In FY 1986, 58 Health Effects Assessment
documents were completed on individual chemicals
to support the program office in setting cleanup
goals  at National Priority List (NPL) sites. At the
request of the program office,  1 6 rapid response
health assessments were provided for use in
emergency situations and for technical assistance/
information on specific chemicals.
  Remote sensing was provided  for site
assessment and pnontization of  sites. For example,
aerial photographic analysis of RCRA work disposal
sites m Illinois (Chicago, Freeport, Sterling, and
Sheffield). Similar projects were  performed in each
region to provide either single data analysis and
assessments at a site or to provide documentation
of change in site conditions. Also monitoring
assisted in the assessment and demonstration of
source and ambient air monitoring equipment and
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analytical methods for abandoned sites. Protocols
for analysis of aqueous samples were evaluated
and protocols for sediment and ground-water
sampling were prepared.
  Engineering staff served on technical advisory
committees, reviewed site assessment and
feasibility plans and advised on engineering issues.
Examples include the technical advisory work
groups for the Iron  Mountain and the Stringfellow
sites and the EPA Ground-Water Modeling Policy
Work Group. A prototype engineering cost model
was developed to test its applicability to remedial
action evaluations. A computer-assisted
engineering design work station was established to
demonstrate the application of this technique. If
successful, the system will save time and cost in
design engineering and the EPA remedial project
management.


7557 Program Outlook
  Scientific Assessment assistance will continue to
be provided to the Regions in FY 1 987. Specific
activities will include preparation of site/situation-
specific risk assessments, rapid response health
assessments and Health Effects Assessment
documents for use in RI/FS and other response
efforts. A central point for coordinating the review
of Regional risk assessments and for providing a
focal point for Regional Offices to request risk
assessment assistance will be established.
  Monitoring will produce protocols for soil
sampling, guidelines for sampling design and
guidelines for monitormg-well construction. Short-
term, quick turn-around technical advice and
reviews will continue to be provided to the Regions
and the Enforcement program.
  Engineering staff members will serve on
technical advisory committees, review site
assessment and feasibility plans, and advise on
remedial action engineering issues. Based on data
collected from surveys, technology evaluations, and
other research activities, evaluations of technology
applicability, feasibility, and costs will be provided
for specific  sites. A report on data requirements for
remedial action technology screening, evaluation,
design, and construction will be completed.
Engineering cost models will be completed to assist
in estimation of the costs of remedial action
alternatives in a remedial investigation/feasibility
study. The application of computer-assisted
engineering design techniques will be
demonstrated for remedial action assessments.
ORD staff will serve on technical advisory
committees, review site assessment and feasibility
plans, and advise on engineering issues.
  Technical advice and consultation on emergency
and remedial response options will be provided to
Regional CERCLA programs and Enforcement
offices. This activity will continue to be conducted
primarily by ORD staff experts to the maximum
extent possible. Technical comments on site and
situation assessment plans and reports will
continue to be a major part of this overall activity.
In addition, a microcomputer-based system to
provide a tool for consistent and reliable cost
estimation will be developed for use in the
Feasibility Study process. The system will be
available to EPA headquarters and regional staff,
state personnel, and contractors for quick, easy
and accurate cost estimation. Engineering will also
continue to develop and demonstrate computer-
assisted engineering design techniques for
evaluation of remedial action alternatives for
uncontrolled hazardous waste sites.


ISSUE:  Personnel Protection
  Personnel protection research in support of
CERCLA activities is directed toward improving the
safety, range, and efficiency of operations, and the
reduction of personnel protection costs. EPA is
obliged to provide those who perform the response
activities at chemical spill and hazardous waste site
incidents with chemical protective clothing,
equipment, and procedures that will prevent
personnel from receiving harmful exposures. Since
all chemcial protective clothing, equipment, and
procedures have limitations, it is important that
these limitations be defined through evaluation and
testing to prevent misuse and personal injury.
Defining the capabilities of personnel protection
technology is also important to promotion of more
efficient operations.

1986 Program Accomplishments
  An  Intra-EPA Workshop  on Personnel Protection
Technology Research/Research Needs was
conducted during  1986. The attendees represented
six regions, the Occupational Health and Safety
staff,  the Environmental Response Team and ORD.
Attendees identified a  number of  areas in need of
research and these will be Jntegrated into future
research program plans. A multiyear contract for
research on personnel protection technology to
support  EPA operational and regulatory programs
was initiated. Development of a test kit for in-the-
field assessment of the degradation and
permeation resistance of protective clothing
materials was initiated. Prototype kits should be
ready during FY 1987.
  An  assessment of the technical and economic
feasibility of decontaminating and reusing
protective garment materials was initiated. An
evaluation of the compatibility of  high-pressure
oxygen with the materials  of construction of a
previously developed self-contained breathing
apparatus was conducted along with an
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 investigation of the state-of-knowledge concerning
 the health effects of routinely breathing high
 concentrations of oxygen. Also, an evaluation of
 heat stress management, including assessment of
 the performance  of vital-signs monitors and
 personal cooling  devices was initiated.
  Interagency coordination of personnel health and
 safety issues has been enhanced by the
 implementation of a Memorandum of
 Understanding (MOD) amongst  EPA, the Coast
 Guard, the National Institute of  Occupational Safety
 and Health, the Occupational Safety and Health
 Administration, and the Federal Emergency
 Management Agency. This MOD covers research,
 development, testing, and evaluation of chemical
 protective clothing, equipment and procedures.

 1987 Program Outlook
  FY 1 987 efforts will include additional testing of
 chemical protective ensembles, evaluation of
 personal cooling  devices and hazard detectors, and
 evaluation, development and verification of field
 methods for rapid on-site determination of
 appropriate chemical protective clothing. Another
 mtra-EPA workshop is planned  as is participation  in
 the MOD Workshop Group. Other coordination
 efforts will continue via participation m ASTM
 Committee F-23 on protective clothing and other
 technical organizations and meetings.


 ISSUE: Reportable Quantities
  Activities under this issue provide carcinogenicity
and chronic effects risk information on specific
chemicals for use by the program office in setting
and adjusting the Reportable Quantity for a
 hazardous substance. The Reportable Quantity is
the amount of a chemical that has to be released
 before the  requirement to notify Federal officials of
the release is triggered.

 1986 Program Accomplishments
  During 1986, Reportable Quantity
documentation, for carcinogenic and other chronic
health effects, was prepared concurrent with
 health documentation efforts for the RCRA
 hazardous waste listing effort for 62 chemicals  and
wastes.  In  addition, draft Reportable Quantity
documents were  prepared for other chemicals on
the original CERCLA hazardous  substances list.
OERR issues revised RQs for 102 chemicals based
on chronic toxicity evaluations prepared by ORD.

 7557 Program Outlook
  Scientific assessment will continue to provide
carcinogenic and other chronic health effects
documentation to support the adjustment of the
 Reportable Quantity for potential and actual
CERCLA hazardous substances  during  1987.
Approximately 90 chemicals/wastes will be
evaluated and documentation prepared
concurrently with RCRA listing activities.
Approximately 100 additional chemicals currently
on the Acute  Hazards List will undergo evaluation
of carcinogenicity and other chronic effects for use
in adjusting their Reportable Quantity. OERR will
propose RQ adjustments for 191 suspect
carcinogens based on evaluations prepared by
ORD.
ISSUE: Technical Support
  Activity under this issue provides expert advice
and technical information to Enforcement, the
Regions and the Program Office for effective
implementation of the Superfund program.

1986 Program Accomplishments
  The scientific assessment program provided
technical assistance to the Office of Waste
Programs Enforcement during 1 987 in the
assessment of exposure, health effects and risk
associated with Enforcement lead Superfund
remedial activities. Assistance was provided on
approximately 20 sites, and activity will continue on
several of these during FY 1 987.
  The environmental monitoring support program
responded to a number of requests for technical
information and assistance during 1 986. These
included performance of a geophysical survey at
the Marrion Landfill  in Region V, a geophysical and
soil organic vapor analysis of the Colber Landfill in
Region X, and review of possible responsible party
reports for the Keystone  Landfill in Region III and
the Cinnammson Study Area of Region II.
  Engineering provided technical assistance to
various EPA Regional offices on the feasibility of
using mobile treatment technologies (e.g., the
Mobile Incineration System, Mobile Soils Washing
System, Mobile In-Situ Containment/Treatment
Unit) for specific cleanup activities in their
jurisdictions. State-of-the-art technology seminars
were developed and presentations made in each of
the Regions. EPA's Mobile Carbon Regenerator,
was prepared for an evaluation to regenerate spent
carbon from a treatment system at EPA's Region IX
Stringfellow site.
  Technical reviews and other advisory activities
were provided on the Beacon Heights, McKin,
Nashua, and Charles George sites in Region I; Byde
Park/Bloody Run, Love Canal, LiPari and
Cinnaminson sites in Region II; the Pepper Steel
and Biscayne Aquifer in Region IV: Indiana Harbour
and Woodville, Wl sites in Region V; Denney Farm
and other dioxin sites in Region VII;  Rocky
Mountain Arsenal in Region VIII, Stringfellow, Iron
Mountain, and Celter Chemical  sites in Region IX;
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and the Western Processing, Bunker Hill and
United Chrome sites in Region X.
  A modeling study was completed on the
performance of proposed remedial action
alternatives at the Western Processing sites in
Kent, WA for Region X. A feasibility study was
completed on the use of mines for the long-term
storage of dioxin-contaminated soils for Region VII
and the state of Missouri.
  Technical support was provided in the
environmental processes area in response to
specific requests from the Regions, Enforcement,
and States on ground-water sampling, analyses,
data interpretation and site-specific modeling.


 7557 Program Outlook
  The scientific assessment program will continue
to provide technical support to Enforcement and to
the Regions by reviewing and/or preparing
endangerment assessments.
  Environmental monitoring will continue to
provide aerial imagery and remote sensing reports
for all sites assessed. Assistance in the review and
testing of site-specific sampling  design, quality
assurance procedures and field audit procedures
will be provided.
  Technical assistance will be provided to EPA
 Regional Offices on the feasibility of using mobile
treatment technologies (e.g., the Mobile
 Incineration System, Mobile  Soils Washing System,
 Mobile In-Situ Containment Treatment Unit) for
specific site cleanup activities. State-of-the-art
technology seminars will be continued in each
 Region. Support will continue to be provided to
 OERR  in the development of a technology base in
support of legislative mandates relative to a final
 rule that adjusts reportable quantities for
designated CERCLA hazardous substances. Efforts
will continue to provide the Program Office,
 Regions and the states with technical support. Pilot
 studies will be conducted on the use of Rotating
 Biological Contactors (RBCs) on  leachates from a
 number of Superfund sites. Technical exchange on
 new, innovative techniques will  continue with
 other countries through formal organizations, such
 as the NATO CCMS, to maximize research
 information generated by outside sources.
   Emphasis will be on technology transfer activities
 such as review and presentation of Superfund
 technology transfer documents to the Regions and
 participation  in many technical conferences and
 seminars. In-house studies will  be initiated on the
 application of biological processes to regenerate
 spent carbon in connection with the Stringfellow
 site. Innovative/newly developed applications for
 treatment of contaminated soils and leachates
 which evolve from site specific (NPL) experiences
 will be provided to all Regional Offices. An increase
in in-house technical assistance to the Regions is
anticipated.
  Technical reviews will continue to be provided to
Regional Offices. Geochemical and geotechnical
assistance will be provided on an extramural basis
and an expanded in-house soils testing laboratory
capability will be initiated. This capability will
provide the Regions with slurry-wall waste
compatibility data, as well as information on
permeability and other soil characteristics. A
modest increase of in-house support will be
provided for a transfer of the latest RCRA protocols
relating to secure landfill design to Superfund
remedial action plan reviews.
  Assistance will continue to Agency and State
personnel on the use of subsurface models,
sampling and testing techniques, and  on the
application of the bioassessment protocols for
determining the toxicity of spilled materials and
Superfund site waste samples.

ISSUE: Technology Evaluations
  The objective of this research is to develop and
evaluate technologies that support emergency
actions at hazardous substance releases and
remedial actions for cleanup of uncontrolled
hazardous waste sites. The technical information
supports the implementation requirements  of
CERCLA to obtain cleanup of the environment,
mitigation of health problems, and recovery of
cleanup costs.

1986 Program Accomplishments
  A handbook on remedial actions at waste
disposal sites was revised and published during
1 986. This handbook is the basic technical
reference for remedial action technologies.  At the
request of Region VII, EPA's Mobile Incineration
System has operated over the past two years at the
Denney Farm site for cleanup of dioxm-
contaminated liquids and soils to demonstrate the
feasibility of this approach to destruction of dioxm.
The Mobile Soils Washing System was designed for
water extraction of a broad range of hazardous
materials from contaminated soils, and has been
prepared for functional system evaluation.
  Detailed technical manuals were completed on
leachate plume management, systems to
accelerate the stabilization of waste piles, cover
systems, stabilization/solidification technology,
drum handling practices, overtopping  control for
impoundments, use of foams for hazardous spills,
and removal/prevention techniques for hazardous
substances releases. Research efforts were
initiated or continued in the area of in-situ
treatment including a field evaluation of
electrokinetics extraction,  bench evaluation of
permeable treatment barriers, bench evaluation of

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aphron technology, and laboratory studies of
grouts, slurry backfill materials and chemical
stabilization techniques.
  Two joint EPA-United States Air Force projects
were completed to demonstrate the full-scale
feasibility of EPA's Mobile In-Situ Containment
Treatment Unit and to evaluate in-situ biological
degradation technology.


1987 Program Outlook
  Efforts will continue to develop and evaluate
techniques for remediation of uncontrolled
hazardous waste site problems with the greatest
emphasis on typical  Superfund wastes (e.g.,
contaminated soil) in two categories: in-situ
treatment and on-site equipment. Four major
technology areas will be developed in each of these
categories:  extraction processes; degradation/
detoxification processes; immobilization processes;
and delivery and recovery processes.
  Extraction processes efforts will include
evaluation of vegetative uptake of organics from
contaminated soils, further research in the use of
artificial freezing for the treatment of contaminated
soils, vapor phase soil decontamination both in-situ
(soil flushing, biodegradation, vacuum removal,
etc.)  and with aphron technology.
  Immobilization processes activities will include
an increased emphasis of solidification/
stabilization evaluations, as well as research in the
areas of grouting, precipitation and thermal fusion.
  Degradation/detoxification processes activities
will include additional research focused on in-situ
processes but with a shift towards developmental
activities rather than field-scale evaluations which
will be the emphasis of the Superfund innovative/
alternative technology demonstration program.
  Delivery and recovery processes activities will
evaluate the effectiveness of delivery and recovery
systems which are essential for effective
implementation of most in-situ processes.
  On-site equipment efforts will be directed
towards field demonstrations and evaluations of
various mobile hazardous waste control
technologies involving flow-through treatment
systems for excavated soils and sludges.
  Studies will be conducted on the mobile
incinerator  and other on-site treatment
technologies to develop efficient feedstock
preparation and handling  schemes in the field.
Combustion Research Facility Tests will occur on
wastes from Hyde Park and Love Canal sites.
  A limited program to demonstrate innovative/
alternative technologies will be initiated in FY
1 987. The objective of this program will be to
develop  reliable cost and performance data on fully
developed cleanup technologies so that their full
commercialization can be accelerated and their use
in Superfund cleanups considered. Technologies
will be selected by the engineering program for use
on sites that are identified by the program office.
Demonstrations will be cost-shared with the
developer paying for the technology and the
demonstration, and the Agency paying for and
conducting the performance evaluation phase. The
Agency will also publish and disseminate the
results of these evaluations.

ISSUE: Innovative/Alternative Treatment
         Technology
   Program components in this issue fulfill the
Agency's responsibilities under Section 311(b) of
CERCLA established by the Superfund
Amendments and Reauthonzation Act of 1986
(SARA) which, in part, establish a comprehensive
and coordinated Federal program of research,
demonstration, and development to promote
commercialization of alternative and innovative
treatment technologies to be used in response
actions. The program is also intended to provide
incentives for development and use of such
technologies.
7557 Program Outlook
   Engineering, in close coordination with the
program office, will operate a large-scale
demonstration program focused on testing  and
evaluation of innovative/alternative treatment
technologies  The objective is to develop  reliable
cost and performance data on fully developed
cleanup technologies so that their full
commercialization can be accelerated and their use
in Superfund cleanups considered. The engineering
program will select privately developed
technologies for demonstration on sites identified
by the program office.  Technologies will be selected
from requests for proposals published in  the
Commerce Business Daily. Demonstrations will be
cost-shared, with the developer paying for the
technology and the demonstration, and the Agency
paying for and conducting the performance
evaluation phase and publishing/dissemination of
results.
  Also in response to SARA, the engineering
program will initiate a  development program that
will focus on evaluating innovative and emerging
alternative technologies developed mostly by the
private sector. These evaluations will provide
credibility to these new techniques and thereby
accelerate their potential for demonstration and
commercialization. Input from a Superfund
technology user's perspective to optimize the
usefulness of the technology will be provided by
coordination of our efforts with the program office.
Technologies will be selected from responses to
requests for proposals in the Commerce Business
Daily.
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  Monitoring will test and evaluate newly
developed, but unproven, innovative monitoring
technologies for their applicability to Superfund site
assessment and pollutant characterization
problems. This will be a cooperative program
between developers of the technologies and the
Agency. In addition, promising, advanced/
innovative monitoring techniques and systems
which are not yet ready for demonstration, will be
evaluated for their applicability to Superfund sites
and further developed so that they can be
validated/demonstrated for use in Superfund
assessments.

ISSUE: Hazardous Substances  Research
7557 Program Outlook
  Program components in this issue fulfill the
Agency's responsibilities under Section 311(c) of
CERCLA created by the Superfund Amendments
and Reauthorization Act of 1986 (SARA). This
section establishes, in part, a comprehensive and
coordinated Federal program of research and
development, the purpose of which is to improve
the Agency's scientific capabilities to assess, detect
and evaluate effects on, and risk to, human health
from hazardous substances.
  An integrated research program, involving the
health effects and scientific assessment offices,
will develop data and procedures to fill information
and assessment gaps which exist in the various
phases of the Superfund public health evaluation
process. The health evaluation process includes the
assessment of toxicity, exposure and dose
assessment and risk characterization. This program
will develop: test methods needed to  evaluate the
hazard potential of waste mixtures; screening
techniques for early detection  of adverse health
effects;  and improved measurement of health
endpoints particularly non-cancer endpomts such
as reproductive effects and neurotoxicity. Predictive
techniques that can reduce the uncertainties in risk
assessment  caused by data limitations will be
developed and data will be generated in response
to specific requests from the Office of Emergency
and Remedial Response. Three research themes
will be emphasized: bioavailabilty/
pharmacokinetics; interactions of compounds in
chemical mixtures; non-cancer health effects.
  The scientific assessment activity under this
coordinated program will include research to
provide data and methodologies for health risk
assessment  use throughout the removal and
remedial processes for the program,  regional and
enforcement offices. Efforts will begin on research
to understand risks posed to reproductive health
resulting from exposure to chemical mixtures, on
the development of methods for treatment of
available exposure information, on the development
of pharmacokinetic models for the exposure
assessment components of risk assessment, on
development of a field guide to assist field
personnel in the application of risk assessment
methods, and on methods to better characterize the
risks from chemical mixtures.
  Health effects research will provide data and
methods in a wide spectrum of health science
disciplines to support hazardous substances
detection and responses and site management
processes. This research program has been jointly
designed with the program office to include
projects that have near-term, direct applicability to
CERCLA waste site issues. In FY 1987,  research
will be initiated for rapid response toxicity testing of
selected waste samples, evaluation of the efficacy
of site cleanups which used hazardous  waste
incinerators by testing the toxicity of residues,
development of dose measurement techniques for
inhaled compounds to provide microdosimetnc and
microtoxicological evaluations in risk assessments,
development of methodologies for predicting
neurotoxic effects from  interactions of complex
mixtures,  utilization of plant sentinel surveillance
systems for detecting contaminated sites, and
development and use of hemoglobin binding as a
dose monitor for human exposure to carcinogens.
  Field screening techniques for contaminants in
soil, water, sediment and volatile organics in
ground water will be developed to accommodate
the increasing data requirements associated with
RI/FSs. These techniques will allow a more
focused, more complete, expedient and cost-
effective field effort during remedial investigations.
The major advantages include rapid turn-around
times enabling cost-saving field decisions, analysis
of a large number of samples in the field, ability to
redirect and focus sampling efforts thereby
increasing the accuracy of estimates of zones of
contamination and shortening field schedules, and
optimum selection of samples for off-site laboratory
analyses.
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Air and Radiation Research Committee

Introduction
  Under the Clean Air Act, the Environmental
Protection Agency (EPA) has three clearly defined
regulatory responsibilities: (1) setting and revising
National Ambient Air Quality Standards (NAAQS),
(2) setting New Source Performance Standards to
limit emissions of NAAQS pollutants, and (3)
setting National Emission Standards for Hazardous
Air Pollutants, compounds not regulated as NAAQS
pollutants but which present a danger to health.
  Primary (health-based) and secondary (welfare-
based) NAAQS are set to protect public health and
welfare from major air pollutants emitted from both
stationary and mobile sources. EPA has set NAAQS
for six pollutants: ozone (03), carbon monoxide
(CO), particulate matter (PM), sulfur oxides (SOX),
nitrogen oxides (NOX), and lead (Pb). Standards for
these "criteria" pollutants must be reviewed every
five years and revised  if necessary. Individual states
are responsible for meeting NAAQS, which they
attempt to do through the development of State
Implementation Plans (SIPs).
  In recent years, some additional environmental
concerns have arisen. One of these involves threats
to the global environment, such as hazards arising
from stratospheric ozone depletion and pollution-
induced changes in global climate. At the other end
of the scale are microenvironments—homes, other
buildings, vehicle interiors. Another major issue is
radon and its potential for causing lung cancer. For
some of these areas, EPA's responsibilities are
limited to technical and public assistance. In other
cases, research is necessary to ascertain the
seriousness of the threat posed and to determine
whether environmental  regulation is an appropriate
response.
  EPA does not have specific regulatory authority
over radioactive materials. The Agency is
responsible for determining public exposure to
radioactive materials and for other advisory and
public assistance responsibilities associated with
radioactive substances.
  In support of the above responsibilities, the Office
of Research and Development (ORD) provides
health and ecological effects data bases,
monitoring and modeling methods, risk
assessments, emission reduction and mitigation
technologies, and the corresponding quality
assurance and technical assistance to develop
regulations. In addition, ORD assists states in
developing SIPs by providing improved monitoring,
modeling, and control technology as they become
available.
  Research performed through the Air and
Radiation Research Committee is managed
according to six research "issues." These issues
focus on regulatory needs and cut across scientific
disciplines and the pollutant-specific structure of
the regulatory program. The following discussion of
ORD's major accomplishments and program
outlook is organized accordingly.

Major Research Issues

ISSUE: Provide Scientific Support to
        Develop and Review Primary and
        Secondary NAAQS

1986 Program Accomplishments
Technical analyses were provided to the Office of
Air Quality Planning and Standards (OAQPS) in
support of their  review of the NAAQS for NOX, CO,
S02 and PM. Comments from the Clean Air
Scientific Advisory Committee (CASAC) of EPA's
Science Advisory Board were addressed in a
second External Review Draft (ERD) of the Air
Quality Criteria  Document (AQCD) for ozone. This
ERD was completed in  November 1985. A follow-
up CASAC meeting was held in March of 1 986 and
a final AQCD was completed in September 1986.
An addendum for the lead AQCD was prepared to
consider blood lead-blood pressure relations. A
CASAC meeting was held in March 1986. A final
addendum was  completed in June of 1986.
  Under the new requirements for measuring
particulate matter less than or equal to 10
micrometers in size (PM-10), studies are being
conducted to develop an appropriate Federal
Reference Method (FRM). As part of this effort, a
study was conducted in Phoenix, Arizona, to
compare the precision and accuracy of two
instruments for  measuring PM-10. Although the
data from this study have not been completely
evaluated, preliminary examination indicates that
measurement discrepancies result from inlet
soiling and the passing of large particles through
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the instrument. Vendors have tentatively agreed
with these observations and are attempting to
correct the problems.
  In 1985, ORD developed a method for measuring
ambient concentrations of non-methane organic
compounds (NMOC) using cryogenic
preconcentration. The method was successfully
employed in multisite field monitoring projects
during the summers of 1 984 and 1985. A third field
monitoring project using the NMOC method was
initiated in the summer of 1986. These field studies
were carried out for OAQPS to obtain data needed
by that office before considering revisions to the
ozone standards. Approximately 20 cities were
monitored in each test. Refinements to the
monitoring methods have  resulted from each of the
first two field tests.
  As a result of documented visibility degradation
in the Western United States, ORD undertook to
develop methods to measure the visibility decrease
and to identify sources of such impairment. This
was accomplished in 1986. In addition, efforts
began to implement a similar measurement and
identification network for the Eastern United
States.
  Quality assurance was provided for air programs
in an effort to ensure that  measurement data are of
known accuracy and precision. A report published
in 1986 indicated that improvements have occurred
in the precision and accuracy of monitoring data
obtained in regional and national measurements.
  Investigators in the Clinical Research Branch of
ORD's Health Effects Research Laboratory
demonstrated significant increases in the
permeability of the respiratory epithelium in normal
volunteers following acute inhalation exposure  to
ozone. This permeability increase is greatest in
those individuals having the largest pulmonary
function responses. There is also evidence that the
epithelial changes may be associated with  local
inflammation. To determine  if this inflammation
arises from acute ozone exposure, additional
studies were begun  using  pulmonary cellular
elements derived from the lungs of volunteers by
bronchoalveolar lavage. Investigators at UCLA,  in
collaboration with EPA, studied the respiratory
effects of long-term  oxidant exposure to determine
the association between the chronic effects and the
magnitude of acute effects of a  single Oa exposure.
  Studies were completed which describe the
amount of ozone removed in the nasal passages
and in the lungs  of individuals while they breathe
ozone. These studies provided information which is
important for the determination of the dose of
ozone reaching target tissues in the lungs and for
risk assessment analysis in humans.
  Significant increases in airway resistance
occurred in a group of moderately exercising
asthmatics exposed to 0.3 ppm N02. This finding is
valuable to Agency regulators who must either set
a short-term standard for NO2 or determine
unequivocally that no such standard is needed.
  Substantial progress was made in a wide-ranging
study of the chronic effects of long-term exposure
to N02 and 03. One-week, three-week, and three-
month exposure regimens were completed and the
data are being analyzed. Additional  animals are
being exposed for 1 2 and 18 months. The study
focuses on the ability of oxidant gases to  cause
chronic lung disease, as reflected by biochemical,
structural and functional changes in the lung.
  Studies of natural killer (NK) cells and mterferon
were developed in 1986. These studies will be
valuable in determining the role of NK cells in
pulmonary immunology. Further, an animal viral
infectivity  model was developed that is  more
analogous to viral infections in man than  are
conventional bacterial infectivity models.  The
former model was used to study the effects of Oa
exposure on host defenses. The oxidant dosimetry
program produced a refined model for the uptake of
ozone in the lungs of  animals, thereby improving
dosimetry comparisons for the ozone risk
assessment to be conducted in 1987.
  Two clinical studies of S02 were completed. The
first study described the concentration-response
range of mildly asthmatic volunteers exposed to
S02 concentrations between 0.25 and 1.0 ppm.
Some asthmatics experienced bronchoconstriction
at S02 levels as low as 0.25 ppm. At 0.75 ppm S02,
50% of the subjects tested had a doubling of airway
resistance. This finding can be important  in
evaluating the margin of safety provided by current
S02 standards. Another clinical study showed that
exposure to a mixture of S02 and sulfuric acid mist
did not affect mild asthmatics more  than exposure
to either chemical alone.
  The basis for the Agency's position on PM-10
was significantly strengthened by simulations that
examined  the influence of breathing route and of
activity levels (from normal respiration to  heavy
exercise) on particle deposition in human  lungs.
  The journal, Science, accepted a paper  which
summarizes current epidemiological, chemical, and
toxicologic evidence relating to lung cancer etiology
in Xuan Wei, Peoples Republic of China. The
toxicologic and chemical evidence is consistent
with the epidemiologic hypothesis that  indoor
smoky coal burning is the prime determinant of
lung cancer in Xuan Wei, especially in women. In
other epidemiological studies, a series of  papers
were published in peer-reviewed journals. These
studies help elucidate the effects of NO2,  particles,
ozone, and passive smoking on lung function,
growth, and respiratory symptoms. Results from
the studies of NO2 and ozone show  effects at low
                                               34

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levels and will be included in the criteria
documents and OAQPS Staff Papers on these
pollutants.
  A study of lead neurotoxicity in children aged
three to seven years indicated that within the range
of 6.3-47.4 milligrams per deciliter of blood there
was a significant negative relationship  between
blood lead level and Stanford-Bmet IQ,  a measure
of cognitive function. The IQ decreased linearly as
blood lead increased. An analysis of audiometric
data from the second National Health and Nutrition
Examination Survey (NHANES-II) was initiated to
investigate the relationship of blood lead levels and
hearing thresholds. Results indicated that the
probability of hearing threshold changes increases
significantly with increasing blood  lead levels at the
frequencies tested (0.5, 1.2, and 4.0KHz). A study
of the neurophysiological effects of lead exposure
in monkeys was also completed and an article
summarizing the findings was accepted for
publication. This study, which is part of a  larger
investigation of the effects of perinatal  lead
exposure, indicated prenatal or postnatal exposure
to lead resulted in abnormal neurophysiological
processing of complex auditory stimuli.
Electrophysiological recordings from specific
auditory areas of the brain showed that lead-
exposed monkeys discriminated between different
sets of auditory stimuli in an abnormal  and
immature fashion.
  Analysis of the 03 data taken by the National
Crop Loss Assessment Network (NCLAN) showed
that substantial dollar  losses could be attributed to
03 damage. In that analysis, water stress during 03
exposure appeared to influence the results. Field
studies were initiated in  an attempt to quantify the
effects of water stress. When completed,  the
revised dose response data will be used to assess
the need to revise the secondary 03 standard. Also
in 1 986, planning was completed for the
International Conference on Assessment of Crop
Loss from Air Pollutants. This conference, to be
held  in 1 987, will include full reports on the NCLAN
research.
  As part of a continuing effort to determine the
causes of visibility degradation, a new method for
measuring  light extinction was developed which
avoids the sight-path problems of a simple
teleradiometer. One study addressed haze trends
over Eastern North America during the 1978-1 982
mini-recession and concluded that improved
visibility and a slight reduction in sulfur emissions
were found in the Northeast in the summer season.
Substantial increases of  sulfur emissions were
noted in the Southeast, with no evidence of
improved visibility. A semi-empirical approach was
developed for selecting chemical and physical rate
parameters to be used in a Monte Carlo regional air
quality model to estimate source-receptor
relationships. This approach is unique on a regional
scale and can be applied to estimate best fit rate
constants for the simulation of sulfur transport,
transformation, and removal.
  Research on the effects of air pollution on
galvanized  steel showed that the corrosion of zinc
results completely from deposited sulfur dioxide. It
was also shown that particle deposition accelerates
the corrosion of zinc.

 1987 Program Outlook
  The research program on NAAQS pollutants will
remain similar in  1 987. Research on the health
effects of carbon monoxide and lead will be de-
emphasized as the focus shifts toward ascertaining
the respiratory, immunologic, and metabolic effects
of long-term and peak exposures to oxidants,
particularly N02. Also, in anticipation of the need to
study the effects of ozone on major deciduous and
coniferous  forests, work will begin on establishing
a laboratory population of suitable trees for future
study.

ISSUE:  Provide Scientific Support to
         Develop NSPS and SIPs

7555 Program Accomplishments
  Two promising procedures for sampling  source
emissions of PM-10 were field-tested. One method
involves the use of a  modified Hi-Vol sampler with
a size selective inlet.  The other uses a modified
dichotomous sampler. Further testing, evaluation,
and refinement are in progress. One of these
methods will be chosen as the standard, based on
its performance during the evaluations. In a related
area, ORD began fabrication of two sampling  trains,
for use by OAQPS, regions, and states.
  Quality assurance was provided to OAR, regions,
other ORD  laboratories, the World Meteorological
Organization, and the World Health Organization
on projects supporting development of NSPS and
SIPs.
  Under the National Audit  Program, audits were
conducted for seven EPA source reference
methods. These were: CO, C02, Oa, Gas Meter
Calibration, S02, NOX, and Coal Sulfur and BTU
content. This program supports development of
EPA regulations and assists regions in determining
stack emissions for SIPs.
  Two manuals, An Operation and Maintenance
Manual for Fabric Filters and Operation and
Maintenance Manual for Electrostatic Precipitators,
were completed and distributed to regional and
state personnel  involved in inspection and
permitting of particulate control systems for electric
utility coal-fired boilers. An interactive computer
model for electrostatic precipitators was developed
which allows prediction of electrical operating
conditions and particle collection  efficiency for any
                                                35

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arrangement of round wire discharge electrodes. A
series of source category reports was completed on
major sources of  PM-10 emissions. These reports
contain  PM-10 emission factors needed by the
states to develop  SIPs. The Sixth Symposium on the
Transfer and Utilization of Paniculate Control
Technology was held jointly with the Electric Power
Research Institute to transfer information and
program results to users and other interested
parties.
  A major achievement in flue gas desulfurization
(FGD) research was the development of improved
calcium  sorbents for low-cost retrofit SC>2 control.
Up to 95% S02 removal has been achieved in a 50
cfm pilot plant using duct injection of dry sorbent in
a humidified flue  gas followed by a fabric filter.  A
Lime/Limestone  Flue Gas Desulfurization
Inspection and Performance Evaluation Manual
was published for use by regional and state
personnel  involved in inspection and permitting of
FGD systems for  electric utility coal-fired boilers.
  The Office of Air and Radiation currently has two
NSPS for industrial boilers in the formal rule-
making process—both under court-ordered
schedules. Both the  NSPS for PM/NOX and for S02
are scheduled for promulgation in early 1987. For
the past year, ORD worked with regulators to
review and acquire NOX emission test data  in an
attempt  to establish the proposed emission
standard based on Iow-N0x burners. However,
insufficient Iow-N0x burner test data were
available to meet the maximum criteria for  standard
setting.  ORD helped regulators develop an S02
standard that is flexible enough to permit the use of
"developing technologies" as alternatives to
scrubbers. The proposed S02 standard includes a
"window" for these  technologies through a 50%
removal and 0.6 lb/106 Btu emission limit criterion.
This window is an alternative in the proposed S02
standard for "developing" technologies to the 90%
removal and 1.2 lb/106 Btu emission limit based on
scrubbers.
  Industrial flares are demonstrated to be effective
in controlling emissions of volatile organic
compounds (VOCs). Source data were developed on
the effect of pilot flames on the performance of
flares. A technical paper summarizing this data was
published  in Combustion Science and Technology.
  An improved mechanism was developed to
quantify the atmospheric formation of ozone from
its precursors (hydrocarbons and NOX). This
mechanism will afford increased accuracy in air
quality simulation models. The first generation
regional oxidant model was evaluated and
improvements are being made. When completed,
this model will be used to evaluate the impact of
various control strategies on ozone air quality for
both episodic (one hour to two days) and long-term
(seasonal to annual) concentrations, in support of
primary ozone standards.
  In support of the proposed inhalable paniculate
air quality standard, the Regional Lagrangian Model
of Air Pollution (RELMAP) was completed. RELMAP
simulates ambient concentrations and wet and dry
deposition of sulfur dioxide, sulfates, and fine and
coarse particles over the eastern U.S. and
southeastern  Canada. A user's guide for the second
pollution episodic model (PEM-2) was completed.
Results of an  evaluation of the PEM-2, an urban
scale  paniculate model, showed that background
concentrations of particles contribute significantly
to urban paniculate pollution. Sulfate sources in
Philadelphia were apportioned using mathematical
and statistical methods.
  A comparison of air quality dispersion models
and receptor models using urban data sets also
demonstrated high background pollutant
contributions  to urban concentrations. AROSOL, an
urban scale aerosol  model, was modified to include
two modules for conversion of sulfate, thereby
allowing AROSOL to be operated either as a
lumped sulfate model or as a model which predicts
the particle size and composition distributions.  In
order to improve model  accuracy, fugitive emission
factors were developed  for use with urban and
mesoscale paniculate models.
  Version Six of the User's Network for Applied
Modeling of Air Pollution (UNAMAP) program was
disseminated to the user community. UNAMAP is a
collection of models and data bases on magnetic
tape which is made available to the user
community through the National Technical
Information Service.
  Version One of the Meteorological Processor for
Diffusion Analysis (MPDA-1) was completed,
resulting in a  format easily used by air quality
dispersion models. An adjustable buoyancy balloon
tracer of atmospheric motion (Phase III) was
improved. The tracer was  developed to evaluate the
accuracy of air pollution transport models and has
application throughout the atmospheric sciences. A
complex terrain workshop was held to review the
preliminary version of the S02 Complex Terrain
Dispersion Model. Several recommendations
emerged from the workshop which will be
incorporated in future versions of the model.

1987 Program Outlook
  The goals for this area remain the same for 1987.
A major area of emphasis  will be research which
focuses on the interrelationship between volatile
organic compounds (VOCs) and air  toxic controls. In
addition, remote monitoring systems will be
developed, evaluated and  applied in areas in which
data are needed for SIP  evaluation  or revisions  and
for Agency evaluation of the need for new
                                               36

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standards. In the area of modeling research, ORD
expects to complete smog chamber studies of
ozone, supporting the development of urban and
regional scale ozone air quality mdoels. In addition,
a user's guide for an evaluated complex terrain
model for stable plume impingement on elevated
terrain will be completed.
ISSUE: Provide Scientific Support to
        Develop Regulations for Hazardous
        Air Pollutants (HAPs)

1986 Program Accomplishments
  Final comprehensive Health Assessment
Documents (HADs) for asbestos and nickel were
delivered to OAQPS. External review drafts on
beryllium, acetaldehyde and dibenzofurans were
released to the public for comment. An addendum
was issued to OAQPS on perchloroethylene, and
assessments were prepared on gasoline and
benzene vapors.
  Tier I Health Effects Summaries were prepared
for 1 2 compounds. They are: propylene oxide,
phenol, styrene, napthalene, ammonia, chlorine,
zinc/zinc oxide, methyl and toluene isocyanates,
xylene, propylene, copper, and mineral fibers.
These documents summarize available scientific
literature on the health effects of a compound. If a
chemical is considered for regulation as a result of
evidence presented in a Tier I document,  ORD will
proceed with the preparation of a comprehensive
HAD.
  Advanced ambient measurement techniques
were investigated, such as combined mass
spectrometry, gas chromatography/Fourier
transform infrared spectrometry, supercritical fluid
chromatography, tunable atomic line mass
spectrometry and cryogenic concentration.  To
improve surveillance and control of industrial
sources, techniques such as capillary column
chromatography, selective detectors, and portable
monitors were investigated. One promising
technique involves the use of specially prepared
stainless steel canisters to inhibit reactions with
pollutants collected. Canister use obviates many of
the problems encountered in using solid sorbents,
such as Tenax, for sample collection. Canisters
have been successfully used in several field
projects and the technique will be further explored.
  Monitoring for VOCs through  the Toxic Air
Monitoring  System (TAMS) continued throughout
the year at Houston, Boston, and Chicago.
Validated TAMS data  have been supplied to OAQPS
and incorporated into their interim air toxics data
base. A second monitoring location in each of the
above three cities has been selected and
monitoring equipment is being installed.
  An earlier study which used the Total Exposure
Assessment Methodology (TEAM) to measure
personal exposures and breath concentrations of
VOCs generated data from 600 individuals. The
data were partially analyzed in 1986. Indoor and in-
vehicle sources were found to be much more
important than outdoor sources, even in the
extremely concentrated petrochemical refinery
areas of northern New Jersey and Los Angeles. The
major source of exposure to benzene and styrene
was cigarette smoking. Chloroform exposure was
primarily due to shower use. Room air deodorizers
and moth crystals were the major sources of
exposure to para-dichlorobenzene. Exposure to
tetrachloroethylene comes mainly from dry-cleaned
clothes. Presentation of the results at the annual
meeting of the  American Chemical Society
(Chicago, September 1985) was reported
nationwide  by the Associated Press and the
nationally televised NBC Today Show. An analysis
of the risks  of organic chemicals in the home was
presented at the annual meeting of the Air
Pollution Control Association in Minneapolis (June
1986). The carcinogenic risks to these unregulated
air pollutants exceeded the risks associated with
the regulated hazardous air pollutants by factors of
10-100.
  The Total Human Exposure Research Council
(THERC) was formed to enhance communication on
research projects concerning human exposures to
chemicals, including hazardous air pollutants.  An
important objective of THERC is to develop a
strategic, five-year plan for all research on human
exposure methodology and assessments conducted
by the Agency. As a first step toward this goal, a
paper was published which summarizes total
human exposure concepts, and a second paper was
published which reviews the Agency's research
program on total human exposure to environmental
pollution.
  An  Interdivisional Air Toxics Study (IATS) was
initiated to  study the health effects of inhaled
HAPs. Compounds under study were selected
based on high  production and potential human
exposure. As part of this effort, studies of p-xylene,
toluene, and phosgene exposures were completed.
The results of one of these studies showed a
concentration-related response of saccharine
aversion to p-xylene in rats, indicating a
neurobehavioral response which warrants further
investigation.
  Important advances were made in developing and
validating test  methods to determine the neurotoxic
potential of HAPs. In particular, studies using
discrete lesions in the visual cortex are beginning
to elucidate the relationships between neural
structures in the visual system and indivdual
components of the flash-evoked potential, which is
                                               37

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commonly used to measure neurotoxicity. The
findings of these studies will provide for a better
understanding of the neurological basis for
neurotoxicity of HAP compounds. Another
significant advance in test method development
was provided by studies to evaluate nervous system
specific proteins (NSSP) as biochemical markers for
neurotoxicity. A variety of NSSPs (synapsin  I, GFAP,
P-38, b-tubulin and N-200) have been shown to
respond to prototype neurotoxicants in a manner
which is consistent with  the accompanying
cytopathology, thus indicating that NSSPs can be
used as biochemical indicators of neurotoxicity.
  A study to evaluate the visual function effects of
the industrial solvent, sulfolane, was completed
and submitted for publication. The results indicated
that high dosages were required to produce effects,
thereby indicating that the visual system is  not
particularly sensitive to this compound. These data
are in contrast to previous work which has shown
that neurotoxic effects such as increased
susceptibility to seizures are produced by lower
dosage of sulfolane.
  More cost effective and better predictive
indicators of reproductive dysfunction are being
developed to evaluate potential HAPs. A series of
papers was published in  peer-reviewed journals
dealing with reproductive effects of manganese in
rats. The results indicated a delay m sexual
development in males with no apparent long-term
reproductive impact. An evaluation of age
dependent gastrointestinal adsorption of Mn304,
which is a combustion product of the fuel additive
methylcyclopentadienyl manganese tricarbonyl
(MMT), was conducted to evaluate the fate of
inhaled particles translocated to the gut. Results
indicate higher absorption and retention in young
(preweanling) rats than in adults, resulting in
greater exposure for younger animals and an
increased possibility of toxicity.
  Two woodstove emission samples showed a
dose-related tumorogenic response in the Sencar
Mouse Skin Tumor Initiation/Promotion Assay.
These two samples are from an airtight woodstove
burning oak or a softwood mixture. This finding is
particularly important in  light of EPA's recent move
to regulate woodstove emissions.
  A preliminary study of seven potential ethylene
oxide control technology concepts for hospital
sterilizers was completed. Acid hydrolysis, low
temperature catalytic oxidation, and adsorption
were selected for follow-up laboratory and field
investigation. Laboratory testing and field
evaluations will be  initiated in  December 1986 to
define the control capabilities of the three
techniques.
  A report entitled "Evaluation of Control
Technologies for Hazardous Air Pollutants"  was
developed to assist state and local air pollution
control agencies in preparing and reviewing
permits for HAP emission. The report was
distributed to OAQPS, all regional offices, and
numerous state and local agencies.
  Two wood stove emission control technologies
are under study: existing catalytic secondary
combustors and advanced non-catalytic secondary
combustion. The catalytic work is focused on two
projects,  both of which will be used to determine
the degradation in emission control performance
over time. Final results will be published following
the 1986-87 heating season. The advanced non-
catalytic secondary combustion development work
is focusing on the use of a small secondary heat
source to maintain a stable secondary flame at the
low, smoldering burn rates commonly encountered
in wood stoves. Lab tests on experimental units of
both types retrofitted into an existing stove show
that this technique is capable of reducing carbon
monoxide and total hydrocarbons by more than
95% compared to this stove's normal emission
level.
  In 1986, ORD conducted a workshop to foster
EPA and industry communication. Partially as a
result of this workshop, Prevention Reference
Manuals  are being developed which will cover how
to evaluate processes and facilities for accidental
release potential, detailed descriptions of pertinent
controls (prevention, protection, and mitigation),
and specific evaluations for individual chemicals.
  Smog chamber studies can simulate a variety of
atmospheric conditions and can provide
information that can be used to predict atmospheric
lifetimes and daughter products. In 1 986, such
photochemistry studies were conducted on several
candidate HAPs, including acrolein, a  compound
currently undergoing regulatory assessment.
  A study was completed on the mutagenic activity
of wood smoke emissions under typical
atmospheric conditions. This study indicated that
the mutagenicity of wood smoke emissions was
enhanced under conditions simulating sunlight.
  The results of a field measurements program on
HAPs were published. This report summarizes the
atmospheric concentrations of a variety of HAPs
observed in selected U.S. cities.
Integrated Air Cancer Program
  Data collected during 1985/86 sampling in
Raleigh, N.C. and Albuquerque, N.M. were
analyzed. Two manuscripts were reviewed and
submitted for publication. One of these describes
effective techniques for measuring the mutagenic
activities of gas and particulate-phase photo-
oxidation products from wood smoke. The other
evaluates the effectiveness of specially coated

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silica gel cartridges for sampling aldehydes and
ketones in the air. Bioassay data were used in the
source receptor modeling analysis for the first time.
Results from the Albuquerque site show that an
average of 50% of the ambient particulate
mutagenicity was from wood stoves and 50% from
automobiles.
7557 Program Outlook
  Hazardous air pollutants are an agency priority
for 1987 and 1 988. Several areas will be
experiencing growth. One of these is total human
exposure monitoring. Efforts will be  made to adapt
the Total Exposure Assessment Methodology to
meet the requirements of the Agency's Air Toxics
Strategy. Ambient and source measurement
methods will be improved to provide accurate data
for use in characterizing source emissions and
ambient concentrations of HAPs.
  Another growth area is municipal  waste
research. Health effects studies will  be initiated in
an effort to determine the risks associated with
emissions from  municipal waste incinerators.
Research will also be initiated to develop efficient,
cost-effective ways to eliminate or control
hazardous emissions from municipal waste
combustors.
  The issue of accidental releases will be
addressed in several ways. A study of hazard
identification and evaluation techniques will begin
as will preparation of reference manuals for key
hazardous chemicals. Technical support will be
provided to regions and states to assist in
preventing such releases or reducing the hazards
once such a release has occurred.
ISSUE: Provide Scientific Support to the
        Mobile Source Regulatory Program

1986 Program Accomplishments
  As recommended by EPA's Science Advisory
Board, validation of the Simulation of Human Air
Pollutant Exposure (SHAPE) and the NAAQS
Exposure Model (NEM) began, using field data
collected during the Denver-Washington, D.C.,
carbon monoxide exposure study. These models
predict human exposure frequency distributions by
modeling human activity patterns and the
concentrations associated with particular
microenvironments. A paper was completed
providing preliminary information on the field
performance of SHAPE and its validation  using the
Denver data base. Additional analyses of the
Denver data base were conducted to determine the
relationship between fixed monitoring stations and
microenvironmental CO concentrations. Additional
analyses were completed which related CO
exposure profiles to estimated carboxyhemoglobin
levels and  measurements of CO in the breath of
subjects. A model for calculating the CO
concentrations in the passenger compartment of
motor vehicles moving in traffic was tested using
the field data from the Washington, D.C.,
microenvironment study. A field study was
conducted in Honolulu to measure CO exposures
while people engage in activities such as
automobile and bus commuting, jogging, shopping,
eating in restaurants, and office work. The findings
suggest that human  exposure to motor vehicle
exhaust in some microenvironments can be a
problem even in Hawaii, which otherwise has
relatively clean ambient air.
  Exposure to carbon monoxide elevates levels of
carboxyhemoglobin (COHb) in the blood. COHb,
therefore,  is a good indicator of CO dose level. In
1986, a study was completed which  showed that
an effects threshold for COHb apparently exists and
lies between the 4%, and 6% COHb levels. This
conclusion was drawn because the investigators
were unable to detect significant physiological or
symptomatic changes in patients with ischemic
heart disease when exposed to CO sufficient to
cause COHb levels of 4% but symptoms were
significantly increased when the COHb was 6%.
Another study of  CO  toxicity was completed in
1 986 which showed  that CO exposure diminishes
hand-eye coordination. These findings are
important for evaluating whether the margin of
safety provided by the current CO standard is
adequate.
  A major  study was completed on the impact of
methanol fuels on evaporative emissions from a car
fueled with 85/15 unleaded gasoline/methanol.
Although the data are still being analyzed, the
results are expected  to have an impact on decisions
about the feasibility of methanol as a popular-use
vehicle fuel.
  Research was conducted to characterize organic
emissions  from motor vehicles operated at reduced
ambient temperatures. The results showed that
formaldehyde emissions did not increase in
gasoline or methanol fueled cars running at idle. It
is hypothesized, however, that further studies,
under actual operating conditions, will show
increased formaldehyde levels as temperatures
drop.
1987 Program Outlook
  The overall approach will remain the same,
particularly in the area of exposure monitoring. A
new research effort will be undertaken, initially
through the Health Effects Institute, to determine
the health effects of aldehydes, especially
                                               39

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formaldehyde, from motor vehicles. In 1988, this
effort will be expanded to include an in-house
component. In addition, research will be conducted
to determine the emission rates of hydrocarbons
from gasoline during vehicle refueling. Studies will
also be done to characterize the emissions from
diesel powered vehicles equipped with advanced
emission control technologies.

ISSUE: Provide the Scientific Data to
        Determine the Impact of the
        Quality of Global and
        Microenvironments on Public
        Health and the  Environment

1986 Program Accomplishments

Integrated Indoor Air Research Program
  EPA has taken a second look at its indoor air
research program and has initiated several changes
as a result.  ORD  and the Office of Air and Radiation
are working closely to develop a  long-range plan for
indoor air. OAR established a new indoor air policy
staff to assist in guiding indoor air research and
decision-making. A review of EPA's plans for the
indoor air research program was conducted by the
Science Advisory Board (SAB). The results of this
review will be available in early 1 987 and will be
used to guide the program in the future. The SAB
was also asked to review ongoing efforts in indoor
air research and  their comments were highly
favorable. Major  program accomplishments are
highlighted be'ow.
  Work was begun on an extensive bibliography of
the world literature on indoor air and total human
exposure, emphasizing concentrations measured in
indoor microenvironments.  The  bibliography will be
completed in 1  987. EPA also developed the
computerized Bibliographic Literature Information
System (BLIS) to  search and retrieve abstracts of
the indoor air quality literature rapidly using an IBM
personal computer. EPA sponsored or participated
in technical meetings to advance knowledge and
understanding  of indoor air quality problems.
  In conjunction  with the federal interagency
Committee on  Indoor Air Quality (CIAQ), a
statistical design was developed for a nationwide
field survey of concentrations of volatile organic
compounds (VOCs) and combustion products in
homes. Although the design was completed,
implementation of the nationwide field survey was
not performed, and a smaller-scale pilot survey was
designed  as an initial step to test hypotheses and to
evaluate,  develop, and refine measurement
methodologies and instruments.
  Protocols were prepared for a chamber study
designed to replicate and extend earlier findings
regarding the neurobehavioral and pulmonary
physiology effects of inhaling VOCs. Additional
research on neurobehavioral effects of VOCs was
begun in several areas including: (1) development
of a portable olfactometer, (2) study of the
possibility of trigeminal sensitivity in Sick Building
Syndrome responders, and (3) development of a
neurobehavioral test battery for use in evaluating
children.
  The exposure portion of a clinical study of
children with parents who smoke was completed,
as were analyses of nicotine in indoor air, blood
and urine cotmme, air and urine mutagenicity,
COHb, particulates, and organics. A pilot field study
was initiated which examines the levels of nicotine
in children of smoking parents and evaluates indoor
levels of nicotine and other pollutants  in the homes
where the children live. A second pilot field study
was completed of the mutagenicity of emissions
from several in-home combustion sources,
including convective and radiant kerosene heaters,
gas stoves, fireplaces, and cigarettes. The data are
being analyzed and will be published within one
year.
  Preliminary studies of organic compound
emissions from  kerosene space heaters were
completed in 1986. Measurements were made of
products of incomplete combustion, and bacterial
mutagenesis bioassays  were conducted on
emission samples. The results suggested that
emissions of carcinogens  may be significant for
certain heater types under specific operating
conditions.
  Laboratory studies of  organic vapor emission
rates from selected indoor building materials and
consumer products were conducted and reported in
1986. Sources studied include a  floor adhesive,
caulking compound, particle board, acrylic floor
wax, moth crystals, and paints. Interlaboratory
comparisons of formaldehyde emissions were
conducted as part of a long-term effort to
standardize emission testing procedures. Several
papers on testing procedures and results were
presented and published.
  A prototype version of a computerized data base
on sources of indoor air pollutants was developed
and distributed for review by a small group of
indoor air quality researchers and the  regulatory
office in 1986. A revised verison will be distributed
for general use in 1987.
Radon Mitigation
  Field testing to develop and demonstrate low-cost
techniques for reducing radon concentrations in
homes was continued. Some of the radon-reducing
techniques being tested are: various natural and
forced ventilation methods, air pressure equilizing
methods, drain-tile suction, block-tile suction,
block-wall ventilation, and sub-slab suction. The
                                               40

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 techniques selected for testing in each home vary
 according to type of house, foundation, local
 geology and meteorology, and other factors.
 Through 1986, 30 homes in eastern Pennsylvania
 and 10 homes in Clinton, New Jersey have had
 radon reduction techniques installed. Reductions in
 most homes have ranged from 90% to 99+%. Based
 largely on the experience in eastern Pennsylvania,
 a brochure for homeowners and a technical manual
 for installers of radon mitigation techniques were
 issued in August 1986. These will be  updated in
 1 987, based largely on results from ongoing field
 projects in Pennsylvania, New Jersey, and New
 York. A Radon Mitigation Test Matrix was
 developed and is being refined to better define the
 size of the future program needed to recommend
 mitigation methods for all housing types on a
 national basis. Initial estimates show a need to
 perform mitigation studies on about 600 existing
 houses and 100 new houses to meet this objective.
 The Radon Mitigation Demonstration Program  is
 being rapidly expanded to meet these objectives
 within a reasonable timeframe.

 Stratospheric Modification
  Initiation of studies on  control technology and
 strategies for controlling  ozone depleting
 substances resulted in identification of sources for
 which additional studies  need to be conducted.
 National and international workshops were held on
 control strategies for stratospheric  modification. A
 workshop was also held specifically on N20
 emissions from combustion. This workshop helped
 to define analytical approaches for  measurement of
 N20 and examined the relative strength of various
 emission source sectors.  The available data
 indicate that stationary combustion sources are the
 major contributors of N20 emissions. Existing
 information has been summarized and evaluated
 and will be incorporated into the information
 provided to the EPA Administrator for use in
 decision-making.


 1987 Program Outlook
  The immediate priority  for the indoor air program
 is to complete a  research needs assessment. A
 preliminary plan for this assessment was presented
 to the SAB in September  1 986 as the cornerstone
for ORD's long-term strategy for studying the
 problems associated with indoor air pollutants. As
this study progresses, research will also continue
 on developing improved monitoring and
 measurement methods, characterizing source
 emissions, developing control technologies, and
assessing the health effects from VOCs and indoor
combustion products.
  The level of effort in the radon mitigation
program will remain stable Stratospheric
 modification research will be increased, to include
 a new research effort to ascertain the extent to
 which changes in global climate may be due to
 man-made pollutants. Interim results are also
 expected from studies of the biological effects of
 increased UV-B radiation resulting from reduction
 of stratospheric ozone.

 ISSUE: Provide Monitoring Support for the
        Department of Energy Off-Site
        Monitoring Program

 1986 Program Accomplishments
  Each year, ORD provides technical assistance to
the Department of Energy in the form of radiation
safety monitoring, long-term hydrological
monitoring, a human surveillance  investigation
program, and maintenance of a radiation data base
ORD also provides radiochemical analyses of
environmental samples for regions, states, and
contractor laboratories. Support to both of these
activities continued in 1986 with a stable level of
effort.


 7557 Program Outlook
  No changes are expected in this program m
1987.


FY 1986 Deliverables

ISSUE: National Ambient Air Quality
        Standards

• Air Quality Criteria Document on Ozone and Other
  Photochemical Oxidants (9/1 5/86)

• Air Quality Criteria Document for Lead (6/30/86)

• Proceedings of an International  Symposium on
  Aerosols (8/28/86)

• Report on  Externalization of Methods to Model
  Visibility Degradation (5/12/86)

• Comparison of Precision and Accuracy Data from
  SLAMS and the National Audit Program in CY-
  83(12/31/85)

• Annual  Report on the CY-84 Ambient Air Audit
  Program (12/31/85)

• Annual  Report on the CY-84 National Audit
  Program (12/31/85)

• Annual  Report on the QA Program for the SLAMS
  Network- CY-83 (12/31/85)

• Report on Audits of Laboratories Making Source
  Measurements (1 2/1 2/85)
                                              41

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• Journal Article on Changes in Pulmonary
  Epithelial Permeability in Man (8/25/86)

• Journal Article on Sensitivity of Neonatal Versus
  Adult Rats to Ozone and Nitrogen Dioxide
  (9/30/86)

• Model of the Regional Uptake of Gaseous
  Pollutants in the Lung (7/25/86)

• Journal Articles on Effects of Air Pollution on
  Symptoms,  Lung Function and Lung Function
  Growth of Children (6/24/86)

• Journal Articles on Dose Effects Function of CO on
  Compensatory Tracking (9/26/86)

• Journal Articles on the Influence of Breathing
  Mode and Activity Level on the Regional
  Deposition of Particles in Man (2/28/86)

• Task Force on Environmental Cancer and Heart
  and Lung Disease (8/27/86)

• NAS Study of the Feasibility of Conducting
  Epidemiologic Research on the Criteria Air
  Pollutants (12/31/85)

• Project Report on the Health  Effects Institute
  Cooperative Agreement (9/30/86)

• Article on Semi-Empirical Evaluation of Regional
  Scale Source-Receptor Relationships (2/28/86)

• Article on Recommended Parameters for Use in
  Regional Visibility Models (11 /27/S5)

• Article on Visibility Improvements Due to a
  Decrease in S02 Emissions During the  1981-82
  Mini-Recession (9/30/86)


ISSUE: New Source  Performance
        Standards and State
        Implementation Plans

• Status Report on Amendment to 40 CFR Parts 50
  and 53 with Respect to PM10 (6/25/86)

• Final Report on Pilot Scale Cement Kiln Test to
  Evaluate Applications  of NOX Control  Combustion
  Modification (12/26/85)

• Interim Report on VOC Destruction Efficiency of
  Industrial Flares (8/27/86)

• Final Report on Evaluation of a Selective Catalytic
  Reduction System of NO* Control on a Stationary
  Diesel Engine (1/31/86)

• Final Report on Application of Reburning on Gas-
  and Oil-fired Package  Boilers (10/31/85)
• Final Report on Field Verification of Wind Screen
  Model for Storage Piles (7/31/86)

• Mathematical Modeling of Single Droplet
  Trajectories in Combustor Flow-Field-FCR Task 1
  Draft Final Report (9/29/86)

• Report on Evaluation of OH Reaction Rate Protocol
  for Determining Reactivity of Organic
  Compounds (9/29/86)

• Report on Numerical Simulations of
  Photochemical Air Pollution in the NE United
  States (6/27/86)

• Report on Development and Evaluation of an
  Improved CBM Mechanism for Urban and
  Regional Modeling (1/15/86)

• Report on EPA Regional Oxidant Model: ROM-1
  Evaluation for 3-4 August 1 979 (5/21 /86)

• User's Guide for Interim (Linear) Regional
  Particulate Matter Model  (2/14/86)

• Report on First Cross Appalachian Tracer
  Experiment (CAPTEX) on Long Range Transport
  of Air Pollutants (4/10/86)

• Report on COM 2.0 (Climatological Dispersion
  Model) User's Guide (11/1 2/85)

• Develop and Distribute UNAMAP Version 6
  (9/2/86)

• User's Guide: A Multiple Source Gaussian
  Dispersion Algorithm Using On-site Turbulence
  Data C\ 727/86)

• Report on Pollution Episodic Model (PEM)
  Evaluation Against Philadelphia Data Base
  (2/10/86)

• Report on Local and Regional Contribution to
  Urban Particulate Matter (7/31/86)

• Report on the Incorporation of S02 Photochemical
  Oxidation Model into AROSOL (7/23/86)

• Report on Air Parcel  Marker System. Phase  III—
  System Testing (5/20/86)

• MDPA-1: A Meteorological Processor for Diffusion
  Analysis User's Guide (1/29/86)

• Report on EPA Complex Terrain Modeling
  Workshop (8/15/86)

ISSUE: Hazardous Air Pollutants

• Final Health Assessment  Document for Nickel
  (9/15/86)
                                              42

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• Final Health Assessment Document for Beryllium
  (4/1/86)

• Tier I Health Effects Summary for Hydrogen
  Sulfide (6/30/86)

• Tier I Health Effects Summary for Chlorine
  (4/30/86)

• Tier I Health Effects Summary for Zinc Oxide
  (3/15/86)

• Tier I Health Effects Summary for Styrene
  (3/7/86)

• Tier I Health Effects Summary for Phenol
  (2/18/86)

• Tier I Health Effects Summary for Ammonia
  (4/30/86)

• Tier I Health Effects Summary for Propylene
  (4/29/86)

• Tier I Health Effects Summary for Methyl
  Isocyanate (7/31/86)

• Tier I Health Effects Summary for Toluene
  Diisocyanate (4/15/86)

• Tier I Health Effects Summary for Propylene Oxide
  (4/15/86)

• Tier I Health Effects Summary for Xylene
  (7/31/86)

• Tier I Health Effects Summary for Copper
  (7/30/86)

• External Review Draft Health Assessment
  Document for Dibenzofurans (7/15/86)

• External Review Draft Health Assessment
  Document for Hydrogen Sulfide (9/1 5/86)

• Final Report on Woodstove/Fireplace  Emissions
  (11/15/85)

• Internal Report on Options for Externalizing the
  HAP QA Repository (5/15/86)

• Journal Articles on Mutagenicity, Carcinogenicity
  and DMA Dosimetry of Polycychc Organic Matter
  from Air (3/26/86)

• Journal Articles on Metabolism, Mutagenicity,
  and Carcinogenicity of Selected PAHs and Nitro-
  PAHs Found in Air (9/30/86)

• Report on the Effect of HAPs (Organic Vapors) on
  Lung Host Defenses in Animals (3/31/86)
• Interim Report on Control Technology for
  Reduction of Hazardous/Toxic Emissions
  (6/2/86)

• Draft Final Report on Effectiveness of Woodstove
  Combustion Chamber Modifications (9/29/86)

• Complete Facility for Wood Stove Lab Certification
  Tests (9/29/86)

• Complete a Detailed Plan for Phase II of the Radon
  Mitigation Research Plan (3/26/86)

• Radon Reduction Approaches for Detached
  Houses: Technical Guidance (4/30/86)

• Provide Engineering Evaluation and Technical
  Assistance to EPA for National and International
  Workshops (9/12/86)

• Report on the Identification, Screening, and
  Measurement of HAP Concentrations in Ambient
  Air (9/30/86)

• Report on Primary Photochemical Processes of
  Acrolein (12/24/85)

• Draft Final Report on Residential Wood
  Combustion Emission Laboratory Measurements
  from Phase I IACP Field Study (1 2/20/85)

• Report on Atmospheric Transformation of
  Emissions from Residential Combustion Under
  Winter-time Conditions (7/23/86)
ISSUE: Mobile Source Pollutants

• Report on New Fuels/Additives Registered in
  FY'85 (12/19/85)

• Complete a Model of Human Exposures Inside
  Vehicles on Highways (3/28/86)

• Article on Characterization of Emissions from Late
  Model In-use, Light-duty Vehicles (10/23/85)

• Article on the Impact of Low Ambient Temperature
  on the Emission Rate of Formaldehyde from
  Motor Vehicles (9/29/86)

• Develop Procedures for Measurement of
  Hydrocarbon Emissions During Vehicle  Refueling
  (12/12/85)
ISSUE: Global and Microenvironmental

• Journal Article on the Effects of UV-B Radiation on
  Competitive Interactions (9/30/86)
                                             43

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• Journal Article on Dose-Response of Marine
  Ecosystems to UV-B Radiation Simulating 0-1 5%
  Stratospheric Ozone Depletion (9/30/86)


ISSUE: Provide Radiological Support to
        DOE

• Annual Report on Off-Site Surveillance Around
  the Nuclear Test Site (5/14/86)

• Annual Report of the Radionuclide
  Intercompanson Studies (6/24/86)

• Report to Headquarters on Externahzation of
  Quality Assurance (5/1 3/86)
                                             44

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Projected FY 1987 Deliverables

ISSUE: National Ambient Air Quality
        Standards
•  Update Evaluation of PM/SOx Health Effects:
   Addendum to the PM/SOx Air Quality Criteria
   Document (1/31/87)

•  Status Report on Evaluation of PM10 Monitoring
   Methodology (12/30/86)

•  Status Report on Evaluation of Improved NMOC
   Methodology (12/30/86)

•  Annual Report on Monitoring Support Provided in
   FY'86 (12/30/86)

•  Interim Report: Establishment and Preliminary
   Results of the Eastern Visibility Network
   (12/30/86)

•  Interim Report on the Status of the Visibility
   Program: FY'86 (12/30/86)

• Annual Report for CY'85 on the QA Program of the
  SLAMS Network (12/30/86)

•  Annual Report on the CY'85 National Ambient Air
   Auditing Program (1 2/30/86)

•  Report on the Reference and Equivalence
   Methods Program (12/30/86)

•  Annual Report on the National Source Monitoring
   Audit Program (12/30/86)

• Journal Articles on the Effects of 03 and N02 on
   Pulmonary Host Defenses in Animals (6/30/87)

• Journal Article on the Immunologic and
   Biochemical Response of Volunteers to 03 and
   N02 (9/30/87)

•  Series of Reports on Various Aspects of Indoor
   N02 and Paniculate Exposure (6/30/87)

• Journal Articles on Pulmonary Function and
   Symptoms in Asthmatic Volunteers Exercising in
   Sulfur Dioxide and Sulfuric Acid (9/30/87)

•  Reports on Health Effects and Sources of
   Summertime Haze in the Northeastern U.S.
   (3/31/87)

•  Journal Articles on Mutagenicity and
   Carcinogenicity of Air Samples from Xuan Wei,
   PRC (9/30/87)

•  Project Report on the Health Effects Institute
   Cooperative Agreement (9/30/87)
• Article on the Use of Streaker to Measure Aerosol
  Composition and Absorption with One-Hour
  Time Resolution (9/30/87)

• Journal Article on Small Particle Soiling of
  Building Materials (9/30/87)
ISSUE: New Source Performance
        Standards and State
        Implementation Plans
• Final Draft Report on Multistage ESP Using
  Prechargers and Collectors with Large Diameter
  Electrodes (5/30/87)

• Report on Fundamental and Bench-Scale
  Evaluations of Promising Dry Injection Sorbents
  and Additives (6/30/87)

• Final Report on Transfer Efficiency Measurement
  Method for Spray Painting in the Surface
  Coating Industry (3/30/87)

• Project Reports on Reduction of VOC Emissions
  from Surface Coating Operations via Process
  Modifications (9/30/87)

• Interim Report on VOC Destruction via Catalytic
  Oxidation (9/30/87)

• Report on Field Measurements of Background
  VOCs (9/1/87)

• Report on Results of Simulated Emissions Control
  Impacts on Seasonal Ozone Statistics (9/30/87)

• Report on Validation of Second Generation Model
  Based on Complete NEROS  Data (6/30/87)

• Report on Evaluation of Alternative to CBM
  Chemical Mechanism for Use in Urban Air
  Quality Simulation Models (3/30/87)

• Report on Development and Evaluation of
  Advanced Lagrangian Model (6/30/87)

• Report on Regional Scale Photochemical Module
  and Sulfate Formation (3/30/87)

• Evaluation and Assessment of UNAMAP
  (4/30/87)

• Article on the Evaluation of  Receptor Modeling
  Results in Philadelphia  (10/30/86)

• Development and Delivery of User's Guide for
  PEM-2 Pollution Episodic Model (11 /30/86)

• User's Guide for Evaluated Complex Terra in Model
  for Stable Plume Impaction (8/30/87)
                                             45

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• Report on Mesoscale Dispersion Study Using
  Tracer Released from 1000 Foot Meteorological
  Tower in Beijing (3/1/87)
ISSUE: Hazardous Air Pollutants
• Experimental Procedures: Measurement of
  Survival of Recombmant DNA Molecules in Air
  (10/30/86)

• External Review Draft Health Assessment
  Document for Acrolein (10/15/86)

• External Review Draft Health Assessment
  Document for Acetaldehyde (11 /30/86)

• External Review Draft Health Assessment
  Document for Phosgene (10/1 5/86)

• Tier One Health Effects Summary for Mineral
  Fibers (10/1/86)

• Tier One Health Effects Summary for Ethyl
  Chloride (10/15/86)

• Tier One Health Effects Summary for Methyl
  Methacrylate (10/1 5/86)

• Tier One Health Effects Summary for Maleic
  Anhydride (10/1 5/86)

• Final Health Assessment Document on
  Dibenzofurans  (10/30/86)

• Project Report on Validation of a Method for
  Measuring Cadmium (1/30/87)

• Report on Development of New Sampling and
  Analysis Techniques for Ambient HAPs
  (12/30/86)

• Status Report on Sampling Conducted at Three
  Locations (12/30/86)

• Internal Report Summarizing Quality Assurance
  for HAP Projects in FY'86 (12/30/86)

• Journal Articles on Neurotoxicological
  Assessment of Hazardous Air Pollutants
  (3/31/87)

• Journal Articles on the Development and
  Application of Micromutagenesis Methods to
  Identify Carcinogens in Ambient Air (10/31 /86)

• Interim  Report  on Wood Stove Catalyst Longevity
  for Pollutant Reduction in Developing Regulatory
  Alternatives  (10/30/86)

• Final Report  on Efficiency Degradation of Wood
  Stove Catalysts (12/31/86)
• Report Describing the Engineering Program and
  Data Base Control of Major Accidental HAP
  Releases (6/30/87)

• Report on Performance of HAP Control Under
  Transient Conditions (1/31 /87)

• Report on a Literature Review Update of More
  Than 1 50 Volatile Organic Compounds (5/30/
  87)

• Report on Atmospheric Depletion Rates of
  Selected HAPS Consistent with Clean Air Act
  Requirements (1 2/31 /86)

• Report on Screening High Volume Emissions for
  Potential HAPS Formed as Transformation
  Products Using SARS (10/30/86)

• Report on Gas/Aerosol Phase Distribution  and
  Structure of Chemical Species in the
  Atmosphere Which May Prove Hazardous to
  Humans (4/1/87)

• Report on the Status of the Integrated Air Cancer
  Project (12/30/86)

• Journal  Articles on the Development and
  Evaluation of Methods to Apportion the
  Mutagenicity of Ambient Air to Sources
  (10/31/86)

• Interim Report on Methods for Determining Wood
  Smoke Source Emissions (9/30/87)
ISSUE: Mobile Source Pollutants
• Report on New Fuels and Additives Registered in
  FY'86 (12/30/86)

• Status Report on Population Exposure Activities
  (12/30/86)

• Article on the Impact of Low Temperature on
  Emissions from Late Model Motor Vehicles
  (9/1/87)

• Article on the Impact of Methanol Fuels on
  Evaporative Emissions (1/30/87)

• Reportto Evaluate FTIR as a Tool for Measurement
  of Exhaust Methanol and Formaldehyde
  (9/30/87)

• Article on Evaluation of Emissions from Diesels
  Equipped with Advanced Emission Control
  Technology (9/30/87)

• Article on the Emission Rate of Mutagenic
  Gasoline Hydrocarbons (3/30/87)
                                              46

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ISSUE: Global and Microenvironmental
• Final Report on Evaluation of the Effectiveness of
  Residential Radon Mitigation for Building Code
  Officials, Home Builders, and Home Owners
  (9/30/87)

• Journal Article on the Nature of UV-B-Related
  Changes in Competitive Balance and
  Mechanisms Involved (9/30/87)

• Journal Article on the Effect of UV-B Radiation on
  Energy and Carbon Cycling Through Plankton
  Species to Economic  Species (8/31/87)

• Biennial Report to Congress on Stratospheric
  Ozone Modification (11/30/86)
ISSUE: Provide Radiological Support to
        DOE
• Annual Report of Off-Site Surveillance Around the
  Nuclear Test Site: CY'86 (7/30/87)

• Annual Report on the Intercomparison Program
  for Radiation Quality Assurance (1 2/30/86)

• Annual Report on Radiation Standards and
  Reference Materials: 1986(12/30/86)
                                             47

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Multimedia-Energy Research Committee

Introduction
  The multimedia energy research and
development program provides the scientific and
technical information necessary to guide the
development and utilization of energy sources in an
environmentally acceptable manner. Research is
conducted: (1) to better understand the
phenomenon of acid deposition and provide
information upon which mitigation decisions may
be made; and (2) to expand EPA's knowledge of the
performance, reliability, and cost of the limestone
injection multistage burner (LIMB) control
technology.
Acid Deposition
  Research on acid deposition (acid rain) is
coordinated through the National Acid Precipitation
Assessment Program (NAPAP), which is
administered by the Interagency Task Force on Acid
Precipitation (ITFAP). The term "acid rain" means
the atmospheric deposition of acidic or acid-
forming compounds in either dry or wet  form.
These compounds exist in the atmosphere as gases
or aerosol particles containing sulfur oxides (SOX),
nitrogen oxides (NOX), hydrogen chloride, sulfuric
acid, nitric acid and certain sulfate and nitrate
compounds. The objective of acid deposition
research  is to develop the necessary data to fully
understand the sources and characteristics of acid
deposition; the extent of damage or potential
damage; and the corrective measures used to
diminish the problem of acid deposition.
Major Research Issues

ISSUE: Man-Made Emissions
  Estimates of high-quality emission data bases
and historical emission trends are needed to
establish the basis for scientific assessments and
policy development. Detailed emission data bases
are required for operation of the Regional Acid
Deposition Model (RADM). State-of-the-art
emission models are needed to develop projections
of future emission trends and costs of alternative
control strategies.
1986 Program Accomplishments
  The 1980 Emissions Data Base and Inventory
was completed. Cooperative efforts with 48 States
to develop the 1985 Emission data base are well
underway. A report on emission trends for SOs and
NOx from 1 900 to 1 980 has been published as have
other supporting studies for developing a
comprehensive emission data base and inventory.
A quality assurance and quality control plan for the
NAPAP Emission Inventory has been published.

FY 1987 Program  Outlook
  The program will continue to develop emissions
data bases derived from mass balance calculations,
new source tests, New Source Performance
Standards (NSPS), and literature reports. We will
continue to develop and test  models specific to
major source sectors (e.g., utilities, other industrial
sources). These models are responsive to policy
options and will be used to analyze cost-effective
strategies.

ISSUE: Atmospheric Processes
  Improvements are needed  in both the scientific
understanding and the field data bases on
atmospheric transport, transformation, and
deposition on acidic substances in order to develop
more scientifically acceptable, yet simplified
models, to meet assessment and policy needs.

1986 Program Accomplishments
  The preliminary evaluation of the full Regional
Acid Deposition Model (RADM) using  the Oxidation
and Scavenging Characteristics of April Rams
(OSCAR) meteorology and wet chemical deposition
data were reported. The gas-phase chemistry
module was compared with both smog chamber
data and the more complex chemical mechanisms.
The RADM cloud processes and aqueous-phase
chemistry module was subjectively evaluated
against limited field data and more complex
models. Because of lack of appropriate data, the dry
deposition module was not evaluated. Much larger
and extensive data bases are required to test the
system thoroughly. Several sensitivity tests were
also conducted in which emissions in the Ohio
Valley were theoretically reduced by 50% and 90%
and resultant calculated deposition patterns were
analyzed.
                                               48

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  A detailed operational plan and feasibility
analysis for the intensive field studies and the
atmospheric model evaluation effort was
completed. Draft performance evaluation
statements and data quality objectives were
provided for  nine experimental tasks required for
the operational and diagnostic model evaluation
field studies. The report indicates that the
operational evaluation of RADM is feasible and the
diagnostic evaluation of the model is currently
being ascertained. Proposed management and
technical approaches are also provided.
  The Regional Lagrangian Model of Air Pollution
(RELMAP) has been applied and evaluated for the
entire year of 1980 as part of the International
Sulfur Deposition Model Evaluation (ISDME).
Assessments have been made of the sensitivity of
the predictions of S02, S04, and total sulfur wet
deposition to the process rates; i.e., the modeled
rates at which S02 transforms to S04 and S02 and
S04 wet/dry deposition occur. The RELMAP
sensitivity studies also address single-layer versus
multilayer model applications.

FY 1987 Program Outlook
  The program will conduct sensitivity testing on
the advanced verison of the Regional Acid
Deposition Model (RADM) using existing
monitoring data bases. We will evaluate and refine
the advanced version of RADM using field study
data. Once completed, RADM will be used to
calibrate Lagrangian models, develop control
strategies, perform source-receptor analysis, and
assess materials damage.

ISSUE: Deposition  Monitoring
  A long-term quality assured monitoring record of
total deposition (both wet and dry) is needed with
sufficient spatial and temporal scale to: (1) provide
data for long-term trend analysis; (2) evaluate
atmospheric models such as RADM and (3)
determine exposure in effects studies.

1986 Program Accomplishments
  The deposition monitoring research  program  is
providing the deposition data on wet precipitation
through the  National Trends Network (NTN). This
1 50-station  network operated  at full capacity in
1 986. A series of data reports covering the first
four years of operation of the deposition were
published.
  Since dry deposition may account for a larger
proportion of total deposition than wet deposition,
implementation of a dry deposition network was
begun with a 5-station dry deposition pilot network.
A contract was awarded for installation of the first
30 sites of a  more extensive network (up to  100
sites)  and field evaluations of proposed
concentration monitors were conducted.
FY 1987 Outlook
  Work will continue on the establishment of an
operational dry deposition network and on the
development of an analytical method which more
precisely measures dry deposition. Dry deposition
characterization and measurements will lead to a
better determination of deposition velocities. An
additional 1 5 dry deposition monitoring sites will be
deployed expanding the network to a total of 45
sites.

ISSUE: Aquatic Effects
  Acidic deposition is believed to be a major
contributing factor to chronic depressions of pH and
possible episodic depressions in aquatic systems.
Effects which  may result include effects on fish and
other aquatic organisms and  on drinking water
quality. The population-at-risk of surface waters
and aquatic biota in the United States is only
partially known. Improvements are needed in  both
the scientific understanding and the field data
bases which define the processes affecting: (1) the
current status of surface waters and watersheds
including episodes; (2) the chemical and biological
changes to those resources; and (3) the rate of
change resulting from current and altered loadings
of acidic or neutralizing substances. These
improvements will allow the development of more
scientifically acceptable, yet simplified relationships
and models for assessment and policy needs.

1986 Program Accomplishments
  The National Surface Water Survey (NSWS)
addresses the current  status  of resources. Results
of Phase I  activity are nearing completion with the
recent publication of data and analysis of the
Eastern Lake Survey and Stream Survey (Pilot), and
the imminent release of the report on the Western
Lake Survey. The Stream Survey (Mid-Appalachian
Region) report will be released in  1987.
  The rate of change of systems is being
investigated by the Direct/Delayed Response
Project. Three different levels of modeling activities
will supply target loading predictions by region.
Results of analyses based on empirical
relationships and single-factor response times for
the Northeast and Southeast will  be released  in
1 987. Verification of prediction of the Direct/
Delayed Response Project (DDRP) will be
undertaken with field and pilot level manipulations
of watersheds as part of the Watershed
Manipulation Project (WMP).  The research plan for
this project was developed and reviewed in 1986 so
that manipulations can begin within 1 987.
  Long-term monitoring provides the ultimate
verification of model predictions by producing
information on water quality trends  especially in
sensitive systems. The Long-term Monitoring
                                               49

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Project was evaluated and redesigned based on the
results of the Eastern Lakes Survey to maximize its
applicability to detecting changes in sensitive
surface waters.

FY 1987 Program Outlook
  Support will continue for the National Surface
Water Survey (NSWS) to allow us to determine the
temporal chemical and biological variability of
sensitive aquatic resources. We will continue the
DDRP which will allow us to predict short-, mid-,
and long-term responses of surface waters to
acidification. The WMP will be continued to allow
us to corroborate the DDRP model predictions at
current and altered acid loadings.


ISSUE:Forest Effects
  Since the early 1 980's, various adverse changes
in forest condition have been observed in the
United States. Apparently increased mortality  has
been observed in high elevation stands of red
spruce and balsam fir. Also there is some indication
that annual increment growth is reduced in these
stands. These observed symptoms are non-specific
and could  be caused by several different factors  or
combination of factors. Acidic deposition and its
associated pollutants have  been  implicated as
causal factors.

1986 Program Accomplishments
  A joint EPA/U.S. Forest Service research
program, the Forest Response Program (FRP),  was
established in 1985 to investigate:  (1) extent of
damage to forest ecosystems which might be
caused by acid deposition, (2) cause and effect
relationships and (3) dose response relationships.
During late FY 1985 and FY 1986, the FRP
established the Spruce-Fir, Southern Commercial,
Eastern Hardwoods and Western Conifer, research
cooperatives. Also, the National Vegetation Survey
was implemented and a Synthesis  and Integration
Team was established. All of these activities have
produced detailed research plans which have
passed peer-review.
  Spruce-Fir Research Cooperative: Following peer
review in  March 1986, a meeting with the Federal
Management Group (FMG) was held  and, as a
result of that meeting and subsequent
reprogrammmg, 21 projects were funded in FY
1986. These projects are addressing  the question
of extent of forest damage  and investigating most
of the major hypotheses of cause and effect related
to the impact of atmospheric deposition on forests.
  Southern  Commercial Forest Research
Cooperative: In FY 1986, three controlled exposure
laboratory studies were funded at Texas A&M
University, Oak Ridge National Laboratory (ORNL),
and North Carolina State University (NCSU).
Controlled exposure-field research was conducted
in FY 1 986 at ORNL and at the Duke Forest Primary
Research Site. In mid-March a request for
proposals was issued with two objectives: (1) to
study plant physiology in natural stands on the
Duke Forest and stand representation of the region;
and, (2) to study the feasibility of field fumigation
techniques. Two projects have been identified for
funding. Four secondary research sites have been
identified and will be established in FY 1987.
Planning for the development of a central testing
facility began in FY 1 986 on a site at the Forest
Service greenhouse facility in Macon, GA.
  Eastern Hardwood Research Cooperative: The
Eastern  Hardwoods Cooperative initiated three
projects in FY 1986. These concentrated mainly on
the spatial extent and temporal development of
adverse changes in forest condition in eastern
hardwood species. Also included are studies
concerning the effects of atmospheric deposition on
physiological  and nutritional processes.
  Western Conifers Research Cooperative: Seven
projects were funded by the Western Conifers
Cooperative in FY 1 986. Similar to Eastern
Hardwoods, the thrust of this cooperative is
problem definition. The concentration of effort in FY
1 986 was on resolution of questions on extent of
damage with  a smaller effort addressing effects
mechanisms.
  National Vegetation Survey: Fourteen projects
were undertaken by the National Vegetation Survey
in FY 1986 exploring the questions of the temporal
development  and spatial extent of changes in forest
condition. These include both analysis of available
data, field observations and two studies along
known deposition gradients.
  Synthesis and Integration: A computerized data
base system was developed to track outputs and
status of FRP projects. The data base will be
expanded to include key bibliographic information
as well as pertinent non-FRP project data. Two
research projects were initiated, both dealing with
the assessment of physiological characteristics for
use in developing models of whole-tree processes
and with the evaluation and development of
statistical techniques for the analysis of
dendrochronological data.


FY 1987 Program Outlook
  We will expand the collaborative research with
the Forest Service on the effects of acid deposition
and other air pollutants on spruce/fir forests in the
eastern U.S. and southern commercial forests.
Quantitative  research will continue on eastern
mixed hardwood forests and western conifers. We
will continue to cooperate with the Forest Service
in the design and implementation  of studies to
determine the extent and magnitude of forest
                                                50

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productivity decline, and will initiate a design to
meet long-term monitoring needs.


ISSUE: Effects on Materials
  The Materials Effects research is directed
towards (1) understanding the quantitative
relationships between the various forms of acidic
deposition and the resulting damage rates to
materials, and (2) identifying the geographical
extent of matenals-at-risk.

1986 Program Accomplishments
  As a result of major program and project reviews
conducted with NAPAP, the materials research
program was reconstructed. A major initiative was
the development of a research program to
determine the effects of acid deposition on paint/
substrate systems.
  A preliminary physico-chemical model of acid
deposition on  galvanized steel was prepared. This
model demonstrated the ability to predict damage
in the field from information gathered in the
laboratory. Additional laboratory and field studies
are being conducted to refine and test the model.
Field studies on other common metals are in
progress at five materials exposure sites. Initial
results of the field study were published that
indicate the sensitivity of metal  surfaces to acid
deposition changes over time, as a corrosion layer
is formed. This has led to the development of a
model of deterioration based on the formation of a
carbonate layer as a rate-controlling step in
deposition.
  The Materials Inventory Data  Base was
extensively reviewed and recommendations
prepared for future work on extrapolating the data
to other metropolitan areas.

FY 1987 Program Outlook
  The program will continue to determine those
materials and resources at risk and to develop data
bases and methods for differentiating the effects of
acid deposition from those of other sources of
pollution. Damage functions will be developed for
selected materials at field sites so that we can
determine if widely used building materials
deteriorate as a result of acid  deposition. An
assessment of the effect of acid deposition on the
service life of  materials and life  cycle costs will be
undertaken.


ISSUE: Assessments
  What existing mechamsm(s) would best integrate
acid deposition research information to provide
policy-makers with the ability to formulate timely
and cost-effective decisions for  dealing  with acid
deposition issues?
7555 Program Accomplishments
  At the beginning of 1986, the assessment
responsibility was transferred from a staff at EPA to
a staff at NAPAP. Ongoing activities were
concluded. Draft assessments in the areas of
emissions, atmospheric processes and source/
receptor relationships, deposition and air quality,
aquatic effects, forest effects, and health and
visibility were completed. In-house analyses to
support EPA programs continue in these areas,
using products of the assessment program such as
a geographical information analysis system.
Limestone Injection Multistage Burner
(LIMB)
  EPA continues to develop LIMB technology that is
designed to reduce both SOX and NOX, the two
major acid deposition precursors. The LIMB
emission reduction technology is designed to be
retrofited to large and small existing coal-fired
boilers.
Major Research Issues

ISSUE: LIMB Technology Development
  Additional information is necessary to document
the reliability and cost-effectiveness of LIMB
technology to reduce the emissions of sulfur and
nitrogen oxides.


1986 Program Accomplishments
  In 1 986, work continued on the development of
high surface area sorbents and sorbents treated
with "promoters" to improve  the sulfur capture
ability of the LIMB technology. The design phase of
the wall-fired full-scale LIMB demonstration was
completed. Also, we continued the laboratory and
pilot-scale research of the LIMB process to improve
engineering  knowledge of the effects of operating
parameters and systems variables associated with
NOx control and S02 capture. As a result of a
Congressional add-on to the LIMB budget, we
initiated a competitive procurement for a
tangentially fired full-scale LIMB demonstration.

FY 1987 Program  Outlook
  In 1987, we will complete the boiler modification
of the wall-fired LIMB demonstration at Ohio
Edison's Edgewater #4 site. After modification we
will begin a full year of testing at the site. We will
evaluate proposals for the tangentially fired
demonstration and continue to support pilot-scale
work. Startup for the tangentially fired
demonstration is projected for 1989.
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FY 1986 Deliverables

ISSUE: Man-Made Emissions

• Proceedings: Second Annual Acid Deposition
  Emission Inventory Symposium (November
  1985), EPA/600/9-86/010, Radian Corporation,
  April 1986.

• Quality Assurance and Quality Control Plan for
  the NAfAP Emission Inventory, EPA/600/8-86/
  025, August 1986.

ISSUE: Atmospheric Processes

• Preliminary Evaluation Studies with the Regional
  Acid Deposition Model (RADM), February 1986.
• Regional Eulerian Model Field Study and
  Evaluation: Proposed Management and
  Technical Approaches, August 1986.

ISSUE: Deposition Monitoring

• Final Report1 Development of Methods for
  Collection and Analysis of Precipitation, June
  1986.

• Precipitation in North America 1984: Annual
  Data Summary, September  1986.

• Annual Report: Spatial Temporal Analysis 1984,
  September 1986.

• Report on Status of Western Core Sites for Dry
  Deposition Measurement, July 1986.

• Report: Siting Selection for  Dry Deposition
  Network, December 1985.

• FTIR Determination of Ammonium and Sulfate
  Ions, September 1986.

ISSUE: Aquatic Effects

• Direct Delayed Response Project, September
  1986.

Volume I:     Executive Summary
Volume II:     State-of-the-Science
Volume III:    Appendices (for State-of-the-
               Science)
Volume IV:    Implementation Plan
Volume V:    Appendices (for Implementation
               Plan)

• Characteristics of Lakes in the Eastern United
  States, Volume I, II, III, EPA/600/4-86/007a, b
  and c, June 1986; National Surface Water
  Survey: National Stream Survey, Phase I Pilot
  Survey, EPA/600/4-86/026, June 1986.
ISSUE: Terrestrial Effects

• Completed Peer Review of Forest Survey Plan,
  April 1986.

• Research Plan for Spruce-Fir Cooperative, April
  1986.

• Research Plan for Southern Commercial
  Cooperative, April 1986.

• Research Plan for Western Confiers Cooperative,
  April 1986.

• Research Plan for Eastern Hardwoods
  Cooperative, April 1986.

• Progress Report from the Southern Commercial
  Cooperative, April 1986.

• Progress Report from the Spruce-Fir
  Cooperative, April 1986.

ISSUE: Materials Effects

• Report on U.S. EPA Workshop on Acid
  Deposition Effects on Portland Cement,
  Concrete, and Related Materials, February 1986.

• Supplemental Statistical-Analysis of North East
  Materials Inventory Data Base, September 1986.

• Report on Development of Acid Damage
  Functions, September 1986.

• Report: Evaluation of Existing Paint Damage Data,
  September 1986.

ISSUE: Limestone Injection Multistage
        Burner (LIMB)

• Status Report on EPA LIMB Development and
  Demonstration Program, EPA/600/8-86/036,
  October 1986.

Projected FY 1987 Deliverables

ISSUE: Man-made Emissions

• Preliminary 1985 Point Source Emissions File
  (1/31/87)

ISSUE: Atmospheric Processes

• Report on Urban and  Mesoscale Modeling
  Methods for Pollutants for Material Damage
  Assessment (12/1/86)

• Report on Chemical Module for the Second
  Generation Eulerian Model (RADM-2) (2/1/87)
                                            52

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•  Report on Sulfur Species Engineering
   Assessment Model and Evaluation Against
   RADM (3/30/87)

•  Report on Review of Existing Mesoscale Models
   for Use in Complex Terrain (3/30/87)

•  User's Guide for a  Mesoscale Acid Deposition
   Model for Assessments Including Model
   Evaluation Report (6/30/87)

•  Detailed Design, Logistics Documentation and
   Sampling and Analytical Manuals for the Model
   Evaluation Field Study (9/30/87)
ISSUE: Deposition Monitoring

• Annual Report on the National Monitoring QA
  for NTN Network, CY 85 (1 2/30/87)

• Site Survey for the Western Core Site (2/28/87)

• Final Report: Development of Real-Time Acid
  Precipitation Monitor (3/30/87)

• Final Contract Report on Development and
  Evaluation of Dry Deposition Monitors (3/30/87)

• Annual Data Inventory Report, CY 1986
  (4/30/87)

• Siting Plan for Dry Deposition Stations in  the
  Southeast. Deliver Final Report (5/30/87)

» Analytical Differences Between Snowfall  and
  Rainfall (9/30/87)

• Trends Analysis of Wet Deposition Data
  (9/30/87)
ISSUE: Aquatic Effects

• Provide Final Report on the Results of the
  Western Lake Survey (1 2/30/86)

• Report on Analysis of Aquatic Long Term
  Monitoring Data (12/31/86)

• Final Analytical Methods Manual for Western
  Lake Survey—I (2/28/87)

• Report on the Application of Flow Injection
  Technology for the Determination of pH in Low
  Ionic Strength Waters (7/30/87)

• Final Report on Critical Evaluation of Biological
  Sensitivity Indices (8/30/87)

• Final Report on Results of NSS-MA/SE National
  Stream Survey (8/30/87)
• Project Report on Regional Rates of Surface
  Water Acidification and Direct/Delayed
  Response(9/30/87)

• Report on Evaluation of QA/QC in the NSWS
  (9/30/87)

• Final Report on Cistern Drinking Water Survey
  (9/30/87)

ISSUE: Forest Effects

• Annual Research Plan for Eastern  Hardwood
  Cooperative (3/31/87)

• Forest Research Plan National Progress Report
  (9/30/87)

• Report on Findings of Mountain Cloud Chemistry
  Project for 1986(9/30/87)

ISSUE: Materials Effects

• Research Plan for Determining the Effects of
  Acid Deposition on Exterior Coatings (2/28/87)

• Field Sampling for Materials Inventory Validation
  (4/30/87)

• Laboratory Study of Acid Deposition of Damage
  Mechanisms on Galvanized Steel (9/30/87)

ISSUE: LIMB

• Final Reports on LIMB Engineering Processes
  Analysis Studies Relating Development Results
  to Industry Comm. Requirements (2/28/87)

• Completion of Boiler Modification and Initiation
  of One Year LIMB Wall-Fired Demonstration
  (7/31/87)

• Draft Report on Initial  LIMB T-Fired Prototype
  Testing (9/30/87)

• Plan and Implement the 1 987 Joint EPA/EPRI
  Symposium on Dry SO2 and Simultaneous
  NOX Control Technologies (9/30/87)
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Interdisciplinary Research Committee

Introduction
  The Interdisciplinary Research Committee plans
those programs in the Office of Research and
Development (ORD) which cross all media. Quality
assurance oversight activities ensure that the data
are of known and documented quality, and that
they meet the requirements established by the
responsible office or laboratory. Risk assessment
management efforts include the development of
risk assessment procedures for ensuring the
consistency and technical competence of the
overall risk assessment program. ORD manages a
series of new intra-agency workgroups which have
developed guidelines for carcinogenicity,
mutagenicity, developmental toxicity, exposure
assessment, and assessment of chemical mixtures.
Exploratory research provides solutions to
environmentally related problems which  require a
basic or fundamental understanding. ORD is
particularly interested in long-range strategic
research issues addressing emerging
environmental problems. Consequently, in addition
to the research conducted through the laboratories,
ORD supports research conducted through its
research grants and centers programs. Efforts
carried out under the auspices of the
interdisciplinary program also include centralized
support for the production and dissemination of
information products, and provide a technology
transfer program to synthesize information and
develop methods of data presentation. Finally,
regulatory support efforts serve to identify major
regulatory issues early in their development and
bring together ORD experts and key  regulatory staff
to discuss the state of the applicable science.

ISSUE: Manage and Implement Exploratory
        Research Grants  Programs and
        Research Centers Program

  This program is designed  to broaden and
enhance the Agency's environmental, scientific and
engineering knowledge base and assure the
application of science basic to the solution of
environmental problems.
  The Research Grants Program is divided into five
environmental program areas: health, biology,
engineering, air physics and chemistry, and water
physics and chemistry. This program division
results in research on a number of such broad
topics as the identification and characterization of
hazardous contaminants in various media (air,
water and soils); understanding of the intermedia
transport, conversion and fate of pollutants in the
environment; human and ecological risk
assessment, incineration and combustion studies,
emissions reduction and control processes m
hazardous waste sites; and development of new
technologies for industrial wastewater treatment.
  Investigator-initiated research applications are
solicited by an annual "Request for Applications"
(RFA). The RFA is a mechanism by which proposals
are solicited for  a one-time competition in a
narrowly defined, high-priority research area.
Grants are awarded on technical merit, potential
relevance to the Agency's long-range research
goals and contribution to a balanced research
program m ORD.
  The Environmental Research Centers Program
supports long-term environmental research m
science and engineering. The program consists of
eight university-based centers, each specializing in
an area of interest to EPA: ground water, hazardous
waste, environmental epidemiology, marine
science, industrial waste, municipal waste,
intermedia pollutant transport, and environmental
risk assessment. Support for each center is
provided through a cooperative agreement with
EPA. Each center's research program is managed
by a center director and an EPA project officer. The
center director is assisted by a Science Advisory
Committee (SAC) which advises the director on the
technical progress of ongoing research and reviews
proposals for further research. The SACs are
staffed by scientists and engineers from industry,
government and academia. At least two scientists
from EPA laboratories are members of each SAC.

1986 Program Accomplishments
  In the Research Grants program, 405 research
applications were reviewed by ad hoc peer review
panels; 1 39 were approved for scientific merit and
evaluated by m-house scientists for agency
relevance. Fifty-four new proposals were awarded
and funding for 49 others was continued. Abstracts
of the new grants awarded m FY 1 986 were
                                               54

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published and widely distributed. A workshop on
genetic toxicology featured the completed work of
EPA grantees. Some major scientific
accomplishments of the Research Grants Program
are highlighted below; a complete annual report, an
FY 1 986 abstract of new awards and an FY 1986
bibliography of published articles from funded
grants are available from the Office of Exploratory
Research upon request.

Major Scientific Highlights
• Validation of an in  vitro fish model technique for
  investigations of metabolism, carcinogenesis,
  and chemical toxicity for better quantitative risk
  assessment and extrapolation from animal data
  to man.

• Establishment of animal models to demonstrate
  correlation between UV-B radiation and the
  onset of melanoma.

• Studies are in progress to elucidate a mechanism
  for  UV-B radiation effects on plants. Efforts are
  focused on the efflux of specific  ions (calcium,
  potassium, etc.) from cells and the influence of
  environmental stresses (such as temperature
  and nutrients) on the UV-B damage.

• The biological detoxification of complex industrial
  mixtures shows promise in the engineering of
  microorganisms which can degrade both
  polychlormated biphenyls and chlorobenzoates.
  Under study is the  mechanism for inserting
  engineered plasmid genes into suitable bacteria
  for  amplification and subsequent degradation of
  industrial sludges,  wastewaters  and
  contaminated soils.

• Development of a new bonded phase for gas
  chromatographic separation of 1 8 priority
  pollutants which are difficult to separate in other
  systems.

• Demonstration that oxygen depletion in sewage
  receiving waters is due mainly to microbial
  utilization of organic carbon rather than to
  nitrification.

• Development of a method, based on supercritical
  fluids, to separate polyaromatic hydrocarbons
  (e.g., benzo-a-pyrene) from particulate water and
  sorbent traps

• Establishment of a  model for the production of (-T,
  NO's and SO-f2 m atmospheric water droplets
  for  use in acid precipitation studies.

• Development of an  effective treatment method for
  removal and control of radon indoor air pollution.
  A promising technique is the use of granular
  activated carbon (GAG) to reduce concentration
  of radon and other radionuclides in household
  water supplies.

• Testing of new, cost-effective drinking water
  disinfection processes. Alternate techniques to
  chlorination, such as the use of ozone, anion
  exchange resins and the coagulation of
  submicron particles methods are being
  investigated.

  In the Research Centers Program, minor changes
were made in several project officer assignments to
provide maximal coverage of centers by the various
ORD laboratories. Each center was assigned a lead
ORD laboratory responsible for conducting
interacting center-ORD laboratory  studies. Detailed
explanations of 1986 accomplishments may be
found in the Research Centers annual reports.

  There were 31 3 deliverables in the centers
program in  FY 1 986, including 99 journal articles,
28 books or bound proceedings, 47 book chapters,
123 project reports, and numerous conferences,
workshops and seminars. Following is a
representative listing:

• Field measurements and laboratory analyses in
  concert with EPA's Ada, Oklahoma Robert S.
  Kerr Environmental Research Laboratory
  (RSKERL) confirmed natural degradation of
  hydrocarbon plumes in ground water.

• Development of stable biofilms of phototropic
  microorganisms used to detoxify organic
  compounds using specific compounds (sodium
  algenate, for example).

• An extensive investigation on the volatilization of
  chloroform and trichloroethylene from bath and
  shower water showed that inhalation of these
  compounds during showering and bathing is a
  significant route of exposure.

• Two new analytical  methods were developed to
  characterize organic aerosols for use in source
  allocation models. Both methods were
  successfully field-tested as part  of the California
  Air Resources  Board's analytical method
  intercompanson study.

• A workshop on environmental risk assessment led
  to alteration in the environmental scoring
  methods being developed by the Office of Policy,
  Planning, and Evaluation.

• Isolation of two new bacterial species which are
  capable of degrading chloroaromatic compounds.
  Experiments showed that these species are
  capable of using trichloroethylene  as their sole
  source of carbon and energy.
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 •  Research on the solubility of gasoline in water
   showed that gasoline octane enhancers increase
   their solubility in water, increasing spill or leak
   hazard.

 7557 Program Outlook
   During FY 1987, the Research Grants Program
 will release a minimum of three RFAs: (1)
 mechanisms of immune alterations induced by
 environmental pollutants; (2) effects of air
 pollutants on forests; and (3) studies of  ozone
 depletion and global climate modification. Five to
 ten grants per RFA will be awarded. Applications
 from individual investigators will continue to be
 peer reviewed and evaluated for relevance to EPA's
 mission. Emphasis will continue on improvement in
 quality of research; coordination with the  Agency's
 research planning process; broad dissemination  of
 results of completed grants, and coordination with
 other Federal agencies. A workshop, "Concepts in
 Inhalation Toxicology," will be cosponsored by the
 Research Grants Program during FY 1987. Other
 seminars and workshops featuring the completed
 work of grantees will be held in Agency
 laboratories and other locations.
   Oversight and management of the Research
 Centers Program will emphasize attention to new
 trends, discoveries or leads coming from ongoing
 studies. An annual summary report of the results
 obtained by each of the centers will be prepared  for
 evaluation of the effectiveness of the center to
                          respond to new research needs. Recommendations
                          for reorienting existing centers will be prepared
                          when appropriate. Symposia and workshops will be
                          held to disseminate information from center
                          studies.
                          ISSUE: Manage and Implement
                                  Distinguished Visiting Scientists
                                  Program
                            The Distmguised Visiting Scientists Program
                          (DSVP) is designed to identify senior visiting
                          scientists who will conduct research in ORD
                          laboratories on a temporary basis, usually one to
                          three years. The intent of the program is to
                          enhance the Agency's scientific competence and
                          quality, to improve relations between EPA and the
                          scientific community, and to increase the visibility
                          of research in ORD laboratories. During the DSVP
                          assignment, EPA provides technical and
                          administrative support as needed.

                          1986 Program Accomplishments
                            During FY 1986, five new DSVP researchers
                          were selected, bringing the total of active DVSP
                          researchers to nine. The five new researchers were
                          selected from a peer review, process of 36
                          candidates. Their names, areas of research, project
                          areas and relevant EPA laboratories are listed
                          below.
   Applicant
   (Institution)
      Winners of the 1 986 DVSP Competition

    Field(s)               ORD Laboratory
                             Project Area(s)
Michael Overcosh
(North Carolina
 State)
Guenther Stotzky
(New York U.)
Richard Dick
(Cornell)
Charles Wurrey
(U. of Missouri,
 Kansas City)

Richard Hawkins
(Utah State U.)
Environmental
 Engineering
Biology
 Microbial
 Ecology


Civil
 Engineering
Physical
 Chemistry
Civil
 Engineering
Watershed
 Science
HWERL-Cincinnati
ERL-Corvallis
WERL-Cmcmnati
EMSL-Las Vegas
ERL-Corvallis
• Engineering methods
  for source reduction
  of toxics and
  hazardous wastes.

• Ecotoxicology

• Fate of genetically
  engineered organisms.

• Classification of
  sludges via physical
  properties.

• Sludge management.

• Measurement methods
  for isomers of dioxins
  and dibenzofurans.

• Responses of
  watersheds to
  acid deposition.
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 1987 Program Outlook
  The DSVP will continue as in 1 986. The
 scientists selected to participate will be renowned
 in their fields of research. Involvement of these
 visiting scientists in the day-to-day activities of
 ORD laboratories will result in exchanges of
 information beneficial to both EPA and the
 scientific community and will enhance the
 reputation and credibility of  EPA research.

 ISSUE: Manage the Agency's Quality
        Assurance Program
  A significant portion of EPA's budget is spent on
collecting environmental data. Quality assurance
(QA) activities play an integral role in the planning
and implementation of environmental data
collection efforts and in the evaluation of the
resulting data.  By means of their QA programs,
EPA organizations can enjoy substantial resource
savings, collecting only needed data and assuring
that the collected data are of the requisite quality.
  Quality assurance is the process of management
review and oversight at the planning,
implementation, and completion stages of an
environmental  data collection activity to  assure that
data provided by a line operation to data  users are
of the quality needed and claimed. Quality
assurance should not be confused with quality
control (QC); QC includes those activities required
during data collection to produce the data quality
desired and to document the quality of the collected
data (e.g., sample spikes and blanks).
  Quality assurance programs consist of specific
activities conducted before, during, and after
environmental  data collection. During the planning
of an environmental data collection program, QA
activities focus on assuring that the quality of the
data needed by data users has been defined, and
that a QC system has been designed for  measuring
the quality of the data being collected. During the
implementation of a data collection effort, QA
activities ensure that the QC system is operating
and that problems found by QC are corrected. After
environmental  data are collected, QA activities
focus on assessing the quality of the data obtained.
Here, one determines whether the data obtained
are adequate to support data-dependent regulatory
decisions or  research hypotheses.
  The Quality Assurance Management Staff
(QAMS) is charged with overseeing the quality
assurance activities of the Agency.

 1986 Program Accomplishments
  The basic elements of the Agency's mandatory
quality assurance program are specified in EPA
Order 5360.1. These include:
 • requiring QA in all Agency-supported  data
  collection activities,
• defining Data Quality Objectives,

• developing quality assurance program and project
  plans,

• conducting audits,

• implementing corrective actions based on the
  audits,

• establishing achievable data quality limits for
  methods cited in EPA regulations,

• developing technical guidelines for assessing data
  quality, and

• providing for QA training.

  Since the issuance of EPA Order 5360.1  in April
1984, QAMS has been intensively involved in
preparing guidance on these key QA program
components. Beginning in FY 1986, QAMS has
begun a transition from the guidance phase to
interactive implementation support. QAMS wants
each Agency data collection organization to
possess the knowledge, resources, and
management support that are essential to  effective
QA program  implementation.
  Specific FY 1986 priorities included:

 1.  Quality Assurance Program Plans—Each
     Agency data collection organization needs an
     approved Quality Assurance Program Plan
     formalizing and documenting the
     management  structure, policies, and
     procedures of its quality assurance program.
     QAMS  has worked with individual
     organizations in order to assure that their
     Program  Plans are carefully designed and well
     implemented. By the end of FY 1986, both the
     pace and the quality of Program Plan
     submissions to QAMS had improved.

 2.  Data Quality Objectives—Data Quality
     Objectives (DQOs) are qualitative and
     quantitative statements developed by data
     users to specify the quality of data needed to
     support a regulatory decision or research
     hypothesis obtained from  a particular data
     collection activity. QAMS  has placed  strong
     emphasis on the development of Data Quality
     Objectives, since they are essential
     precursors to  the design of a meaningful QA/
     QC program for data collection. In FY 1 986,
     QAMS  pursued the following emphases
     related to DQOs:

    • working  directly with individual programs to
     help them develop solid DQOs for selected
     data collection activities,
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     • in cooperation with the Office of Policy,
       Planning, and Evaluation, developing an
       Agency-wide tracking mechanism for DQO
       preparation, and

     • preparing a training workshop on DQO
       concepts and procedures.

QAMS' overall goal has been to direct the attention
of senior management to the importance and value
of the DQO process.

 3.   Management Systems Audits—
     Comprehensive auditing is critical to the
     success of the Agency's quality assurance
     program. Although there are several major
     categories of QA audits, the one which QAMS
     emphasized most in FY 1986 is the
     Management Systems Audit (MSA). The MSA
     is a tool for measuring the implementation of
     a QA program against the individual
     organization's management structures and
     standards described in  its approved QA
     Program Plan. QAMS' FY 1986
     accomplishments in this area were threefold:

     • QAMS provided formal guidance as well as
       informal support and training to Agency
       organizations carrying out their own
       internal Management Systems Audits.

     • QAMS conducted an extensive independent
       MSA of the Region V quality assurance
       program. (A report on this audit will be
       available early in FY  1987.)

     • QAMS commenced a comprehensive
       analysis of the difficult conceptual problems
       associated with quality assurance audits
       focused on Office of  Research and
       Development activities

All of these achievements were directed at the
ultimate goal of making MSAs a routine but
powerful oversight tool for identifying the strengths
and weaknesses of Agency QA program operation.
 1987 Program Outlook
  During FY 1 987, QAMS' transition from the
 guidance phase to implementation support will
 continue. The long-term goal is to institutionalize
 line management responsibility for assuring the
 quality and utility of the Agency's data collection
 programs. Specific FY 1987  priorities include:

 1.  Establish the DQO process as the first key step
    in designing major new  data  collection
    programs.
 2.  Assure that all Agency data collection
    organizations are supported by program plans.

 3.  Establish both internal and external
    management systems audits as a standard
    and effective element of the Agency's QA
    program.

 4.  Implement a comprehensive QA training
    program/workshop to meet the information
    needs of all participants in the Agency-wide
    QA program.

 5.  Implement an alternative  method validation
    procedure to take advantage of data collected
    during laboratory QC analyses.

 6.  Analyze the procedures for developing QA
    project plans, and make subsequent revisions
    to clarify responsibilities and streamlining the
    review process whenever appropriate.

Fulfillment of these priorities will enhance EPA's
progress toward its ultimate goal of assuring
scientifically and legally defensible environmental
data in support of its regulatory, enforcement, and
research objectives.

ISSUE: Assure Uniform  Risk Assessment

  EPA's scientific assessment program provides
uniform  Agency-wide  guidance to assure the
consistency of exposure and  risk assessments to
support  regulatory decision-making.
  Final risk assessment guidelines will be
published on carcinogenicity, mutagenicity,
developmental toxicity, complex mixtures, and
exposure. Additional guidelines on reproductive
effects, systemic toxicity and pharmacokinetics are
under development. A new Agency-wide Risk
Assessment Forum provides a  mechanism for
interoffice exchange on  science issues in risk
assessment; advises the Agency on precedent-
setting cases and important environmental risk
assessment issues, and recommends revisions or
updates to the risk assessment guidelines A
computer-housed electronically communicated
catalogue of Agency risk assessment  and risk
management information on chemicals will be
managed by the scientific assessment program.

1986 Program Accomplishments
  Five risk assessment guidelines were approved
by the Administrator and were published in Vol. 51
of the Federal Register on  September 26, 1 986:

• Guidelines for Carcinogen Risk Assessment, pp.
  33992-34003.

• Guidelines for Estimating Exposures, pp. 34042-
  34054.
                                               58

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• Guidelines for Mutagenicity Risk Assessment, pp.
  34006-34012.

• Guidelines for the Health Assessment of Suspect
  Developmental Toxicants, pp. 34028-34040.

• Guidelines for the Health Risk Assessment of
  Chemical Mixtures, pp. 34014-34025.

  The guidelines and the public comment were
reviewed favorably by the Science Advisory Board,
and revisions requested by the Science Advisory
Board were made. An ad hoc Agency-wide group is
working on an implementation plan for the new
guidelines. In FY 1 986, the Agency continued work
on separate guidelines for male infertility and
female infertility, scheduled proposal in the Federal
Register for FY 1 987, and initiated work on new
guidelines on the use of measurement information
m exposure assessments, and on
pharmacokinetics.

  The Reference Dose (RfD) Workgroup on
acceptable daily intakes completed and made public
risk assessment information on 98 chemicals.

  The Integrated Risk Information System (IRIS), a
computer-housed electronically communicated
catalogue on Agency risk assessment and risk
management information for chemical substances
was developed and implemented. Risk assessments
for non-carcinogenic health effects for 98
chemicals have been input into  IRIS. IRIS contains
information (toxicity and regulatory) in a chemical
specific format and chemicals are selected for
inclusion based on both their lexicological
properties and their occurrence in the environment.
IRIS was designed especially for Federal, State and
local environmental health agencies as a source of
the latest information about Agency  health
assessments and regulatory positions for specific
chemicals

1987 Program Outlook
  Risk Assessment Forum activities for FY 1 987
are as follows.

• The RfD workgroup will complete and make public
  risk assessment information on approximately
  200 chemicals in addition to those completed in
  FY 1986.
• A draft report on several issues involved in
  assessing carcinogenic risk from oral exposure
  to arsenic is  nearmg completion and being
  readied for a peer review workshop in early FY
  1987.

• A draft report on the appropriate safety or
  uncertainty factors for cholinesterase inhibitors
  will be ready for review in early FY 1 987.
The following reports were completed and are
being published:

• Interim risk assessment procedures for mixtures
   of chlorinated dibenzodioxins and dibenzofurans.

• Formal report on "Proliferative Hepatocellular
   Lesions of the Rat: Review and Future Use in
   Risk Assessment (neoplastic nodules)." All
   internal and external reviews are completed and
   it will be printed early in FY 1 987.

• Interpretation of Neoplastic Nodules

  The IRIS activity is expected to include risk
assessments for noncarcinogenic health effects for
approximately 200 additional chemicals and
carcinogenic health effects for 100 chemicals.

ISSUE: Technical Information Product
        Management/Technology Transfer
  The Center for Environmental Research
Information (CERI) provides centralized support for
the production of information products in a cost-
effective manner, insures consistent uniform
dissemination of research results, and provides a
technology transfer program to synthesize
information and develop presentations to more
effectively support specific high-priority program
objectives at the lowest cost to the government.

1986 Program Accomplishments
  In 1 986, CERI continued to provide support to
ORD laboratories by writing summaries of research
projects conducted by or for ORD, editing
documents and  summaries, assuring the quality of
material submitted for printing, typesetting and
producing documents, assuring the quality of and
preparing documents for submission to the
National Technical Information Service, controlling
the distribution of documents, and responding to
requests for  publications and documents.
  The technology transfer program assessed the
status of research and regulations, discussed with
the Research Committees their priorities for
disseminating material, developed innovative
information transfer mechanisms, and ensured that
information on improved technology and
management practices was distributed to
appropriate audiences to comply with EPA
regulations. All information on products was
developed using a team of participants from ORD,
EPA program offices, and private industry.
  In 1986, CERI developed and published two
manuals, two handbooks and a variety of brochures
and information tools for the technological and
regulatory communities.
  The design manual. Municipal  Wastewater
Treatment, provides a comprehensive source of
                                              59

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information to be used in the design of disinfection
facilities for municipal wastewater treatment
plants. The manual includes design information on
halogenation/dehalogenation, ozonation, and
ultraviolet radiation.
  The manual entitled Fabric Filter Operation and
Maintenance is slanted toward the concerns of the
plant environmental engineer responsible for long-
term control strategies, O&M plans, preparation of
bid specifications, and performance trends analysis.
The document also presents information to enable
plant personnel to recognize potential problem
areas as well as existing problems, their underlying
causes, and their solutions. The  information
provided should help EPA field personnel to
determine if the fabric filter is operating within the
applicable regulations, to judge the effectiveness of
the plant's O&M program, and to assess the causes
of poor fabric filter performance.
  The handbook entitled Permit Writer's Guide to
Test Burn Data—Hazardous Waste Incineration
was developed for State and Federal permit writers
and others concerned with the permitting and
testing of hazardous waste incinerators. The
handbook summarizes the test results from
hazardous waste burns conducted at 23 full-scale
stationary incinerators. In addition to the
incinerator data, the handbook also presents the
results of hazardous waste test burns at 11 lime,
cement, and aggregate kilns and 11 industrial
boilers.
  The handbook entitled Stream Samp/ing for
Waste Load Allocation Applications discusses
sampling strategies that will facilitate the fine
tuning of water quality models for allocating waste
loads.
  Other publications produced this year describe
radon reduction techniques for detached houses,
nitrogen oxide control for  stationary combustion
sources, the technology of sequencing batch
reactors, the causes and control of activated sludge
bulking and foaming, and the National
Pretreatment Program  These publications help
industries and municipalities to understand
applicable regulations and the available
technologies to satisfy those  regulations.
   In addition to publications that aid EPA
constituencies, CERI develops seminars to transfer
information to appropriate users. In 1986, the
seminar series  stressed current technologies for
treating hazardous waste in keeping with RCRA
and CERCLA regulations. These seminars were
intended not only to transfer information  but to
establish lines of communication so that the Office
of Solid Waste, the Office of Research and
Development, EPA Regional Offices and the states
could avail themselves of all  resources for
accomplishing their missions.
1987 Program Outlook
  In the ensuing year, CERI plans to realize 11
projects in hazardous waste treatment and
disposal, ground-water contamination, drinking
water and air pollutant regulations, and municipal
treatment of wastewater, each of them calling for
seminars or guidance documents, depending on
audience needs. As an example, CERI plans to
address the problems associated with RCRA
regulations that pertain to the contamination of
groundwater by hazardous wastes migrating from
sites where they are stored. Since site owners
must control releases of these wastes they need to
know how to investigate and assess whether
corrective action is required and what technological
alternatives are available and appropriate for
resolving the problem. CERI is therefore conducting
10 technology transfer seminars and developing an
associated publication that addresses preliminary
assessments, site investigations, and corrective
action alternatives. The information will be targeted
at owners and operators of hazardous waste sites,
as well as federal and state regulatory officials who
are involved with administrating RCRA regulations.
ISSUE: Regulatory Support
  The Office of Regulatory Support (ORS) was
established in FY 1986 to sharpen ORD's focus on
regulatory development in the Agency and to serve
as a bridge between scientific/technical expertise
and regulatory decision making. ORS' primary
function is to identify major regulatory issues early
on in their development and bring together ORD
experts and key regulatory staff to discuss the state
of the applicable science. By helping to delineate
the areas of relative certainty and uncertainty,  and
by identifying areas of research which hold
promise, ORS seeks to improve the ability of ORD
to make a very direct and timely impact on
regulatory decisions.

1986 Program Accomplishments

• Created a  new organizational unit comprised of
  seven professionals and a director from diverse
  regulatory and scientific backgrounds.

• Established strong working ties with key staff in
  EPA program offices.

• Organized several workshops to bring regulatory
  and scientific personnel together to reach an
  understanding of the applicability of selected
  research to critical regulatory decisions.

•  Provided weekly status report to ORD senior staff
  on  key legislative and regulatory development
  activities.
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 • Organized information on all ORD workgroup
   activities.

 • Represented ORD on the Agency steering
   committee and the Administration Risk
   Management Council.

 • Organized briefings for the AA on all options
   selection reviews and related regulatory
   decisions.

 • Represented ORD in critical Agency-wide task
   groups including the integrated chlorinated
   solvents strategy, CERCLA Title III planning,
   Superfund health program development, etc.

 • Developed a series of issue papers informing  ORD
   of regulatory and technical issues on the Agency
   regulatory agenda.

 7357 Program Outlook
  ORD has identified  the following regulatory items
 as those which will warrant significant ORD
 involvement in the coming fiscal year. Staff will
 employ a variety  of means to ensure ORD's
 participation in the deliberations leading to
 regulatory decision-making.

 Resource Conservation and Recovery Act

 • Leaking underground storage tanks: regulations
   for tanks

 • Restriction of land  disposal of certain hazardous
   wastes—solvents and dioxins; California list—
   and establishing the framework for EPA's land
   disposal program.

 • Air emission regulations for hazardous waste
   treatment, storage  and disposal facilities.

 • Proposed reinterpretation of mining waste
   exclusion—smelting/refining.
 •  Corrective action at Federal facilities.

 •  Corrective action regulations for  underground
   storage tanks.

 •  Criteria for classification of solid  waste disposal
   facilities and practices (revision).

 •  RCRA location standards for hazardous waste
  treatment, storage  and disposal facilities.

Superfund (CERCLA)

 •  National Contingency Plan—Hazard Ranking
   System revision.

 • Title III Emergency  Planning and  Community
   Right-to-Know.
• Research, Development, Demonstration and
  training amendments.
Clean Water Act

•  Sewage sludge use and disposal regulations.

•  Regulations to implement recommendations of
   the Domestic Sewage Study.

   Comprehensive revision to ocean dumping
   regulations.


Safe Drinking Water Act

•  Proposed National Primary Drinking Water
   Regulations: inorganic and organic compounds
   and microbiological contaminants.

•  Final National Primary Drinking Water
   Regulations: maximum contaminant levels for
   volatile organic chemicals found in drinking
   water.

•  Proposed National Primary Drinking Water
   Regulation: surface water treatment criteria.

•  List of contaminants occurring in public water
   systems which may require regulation.

•  Criteria for state implementation of wellhead
   protection program.
Clean Air Act

• National Ambient Air Quality Standards: carbon
  monoxide, particulate matter, sulfur oxides,
  nitrogen oxides, ozone.

• National Emissions Standard for Hazardous Air
  Pollutants—organics.

• Control  of excess evaporative emissions/fuel
  volatility.

• Gasoline marketing control strategy.

• Treatment, storage and disposal facility area
  source air emissions—RCRA standards.
Toxic Substances Control Act

• Testing rules and standards, Section 4—various.

• Biotechnology rule—significant new use rule;
  pre-manufacturmg notification and reporting
  requirements.
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Federal Insecticide, Fungicide, and
Rodenticide Act
• Strategy for regulation of inert ingredients in
  pesticide formulations.
• Special and routine reviews of registration/
  reregistration.
Cross Media Issues
• Strategy for control of municipal waste
  combustion.
• Stratospheric ozone protection plan.
• Agency indoor air quality policy.
• Air toxics strategy.
• Risk assessment guidelines implementation.
• Integrated Environmental Management Program.
• ORD Regional Communication Study.
• Regulatory endpomts for environmental risk
  assessment.
• Implementation of chlorinated solvents
  integration strategy.
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Water Research Committee

Introduction
  EPA's water research program provides the
knowledge and methods required to protect our
Nation's freshwater and marine environments, to
ensure the continued safety of our drinking water
supplies, and to implement the most cost-effective
wastewater treatment technologies. Demands on
water supplies are increasing while chemical
contamination from toxic wastes and waterborne
diseases are posing major threats to some
localities. Traditional methods and strategies to
measure and control pollution effects, especially
from organic chemicals, may no longer provide the
level of assurance demanded by the public. In order
to meet the challenges of increasingly complex
contaminants in water,  research must develop
effective  approaches to  assess a growing number
of potentially harmful mixtures of organic,  toxic and
chlorinated organic compounds Water
management is becoming more complicated, and
regulators in both the federal and state sectors
require greater scientific certainty as a basis for
their decision.
  In this  context, EPA's health effects research is
important to the development of both drinking
water and ambient water quality regulations. The
engineering research program's evaluation,
development and transfer of innovative treatment
technologies to municipalities, industry and private
landowners assists in the  implementation of cost-
effective  alternatives. EPA is also accelerating its
research into the toxic impacts to fish, wildlife and
their ecological systems. Finally, the necessity for
credible research and monitoring data is a  cross-
cutting issue of significance to the entire research
program.
  Water  research activities will continue to provide
support to the Agency in the following areas.
developing revised and  new drinking water
Maximum Contaminant Levels and Health
Advisories; developing Criteria Documents and the
scientific underpinnings of ambient  water-quality
regulatory policies; assisting the  Regions and
States to meet the burgeoning demand for toxicity-
based National Pollutant Discharge Elimination
System (NPDES) permits; providing technical
support to the municipal wastewater construction
program in pretreatment, sludge, infiltration/inflow
and protecting ground water resources.
  The six topics described in this report represent
the principal concerns in the water research area.
Specific research outputs supporting each topic
appear in the final section of this water research
committee report

Major Research  Issues

ISSUE: Water Quality Based Approach/
        Permitting
  A continuing issue in water quality regulation is
the water  quality based approach (WQBA) program
which focuses on the quality of ambient marine
and freshwaters essential to protect human health
and aquatic life Emphasis is now placed on
characterizing the attainable uses of a water body
based on  natural features and surrounding land
forms, single chemical criteria development and
toxicity reduction through biomonitoring.
Determination of wasteload allocation is based on
these factors. Diffuse or nonpoint sources of
contamination are addressed through best
management practices. These activities are
supported by permit and receiving water
monitoring, methods standardization, and quality
assurance. Measurement of toxic concentrations of
chemicals m water, sediment and tissue is
required. Speciation of elements, metallic
compounds,  and PCBs by GC/MS are to be
included in these measurements.

1986 Program Accomplishments
  During FY  1986, the health research program
continued to develop and evaluate health effect
bioassays  for the National Pollutant Discharge
Elimination System/Water Quality Based Approach
(WQBA) permitting program. Research has been
conducted to enhance our knowledge of collection,
concentration, and bioassay techniques for
evaluating the health effects of wastewater
effluents. Modifications to existing protocols for the
Salmonella mutagenicity assay and the Chinese
Hamster Ovary cell cytotoxicity assay have
increased the sensitivity for detecting these
biological endpoints  in whole wastewater samples.
Using this modification, mutagenicity and toxicity
were detected in wastewater, and these effects
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were not previously detected using the standard
protocol. A preliminary finding has demonstrated
that over 80% of the mutagenic activity of
wastewater effluents was identified in the
particulate matter. Determination  of the physical
state of mutagenic components of wastewater
would  have a significant effect on  the assessment
and management of the disposal options to protect
human health.
  A site has been selected on the  York and James
Rivers  to study the relationship between  microbial
indicators of water quality and disease in clam and
oyster  consumers. Background measurements of
various microbial indicators have been made in the
harvesting waters and in  the shellfish meats prior
to beginning the human clinical study. OMB
approval to conduct the human clinical study was
obtained in the  last quarter of the  year.
  Manuals were published for the  identification of
marine invertebrates and phytoplankton.  Improved
alternative media for total coliform bacteria were
developed and validated in an mterlaboratory study.
Mammalian cell culture methods for virus analysis
were updated and published to replace dated
material in the existing (1 984) USEPA Virology
Methods Manual
  Effects of holding time and temperature on
coliform testing for waters was published, as was
an improved method to reconstitute waterborne
viruses and revised urology methods on sample
cytotoxicity removal. User friendly  data reduction
PC packages were provided for use by Regions
  In the area of monitoring and quality assurance,
three reference ualibrations were added to the
repository of biological toxicity testing systems,
single laboratory precision/accuracy testing was
performed for biological and chemical test
procedures; and reference materials and
performance audit materials were  supplied to
support freshwater analysis. Performance
evaluation studies were conducted for trace metals,
minerals, nutrients, PCBs, pesticides, volatile
organics, cyanides, residues, and oil and grease
Biological quality control samples,  calibration
standards, and other quality control samples were
distributed
  The environmental processes and effects
program has completed several major projects in
1986. These include completion of: a report on
analytical components of  a field study to test
wasteload allocation (WLA) models; workshops on
selected WLA models and the development of
user's  manuals for two models; a map of aquatic
ecoregions for the coterminous United States and a
report on methods to measure bioaccumulation
from pollutants  in sediments.  Several  case  studies
using toxicity tests of complex effluents were
finished. An evaluation was conducted of the
toxicity factor model to derive site-specific criteria,
and evaluation of site-specific ammonia criteria for
a field site was carried out. Ambient water quality
criteria were completed for nickel, Dursban,
pentachlorophenol, dissolved oxygen, toxaphene,
selenium and zinc. In  addition, several ambient
water quality advisories were also prepared.


7557 Program Outlook
  The health research program will continue to
generate data which is suitable for performing
assessments of the potential health effects from
wastewater  effluents.  Assessment of the relative
potency of a given wastewater effluent will be
based on biological data in comparison with
relevant reference chemicals and environmental
mixtures for which there are documented  effects. A
report on the evaluation of health effect bioassays
methods for  testing wastewaters will be published
  The human clinical study to determine the
relationship  between microbial water quality
indicators and disease in shellfish consumers will
begin. The first year will concentrate on oysters. In
FY 1988, clams will be used in the clinical studies
  In the scientific assessment area, 10 human
health chapters for Ambient Water Quality criteria
documents will be prepared  Ten water quality
advisories will also be  developed for OW Reviews
of four 301(g) variance requests will be completed.
Response to technical  assistance requests will
continue.
  The monitoring and  quality assurance program
will continue to emphasize the generic
instrumental approach in order to provide  the most
cost-effective methods possible. This will include
continuing work  on GC/MS and HPLC/MS
methods for  organic chemicals, ICP methods for
metals and ion chromatography for specific ion  and
metals speciation in ambient waters, municipal
sludge, sediments and biological tissue  Research
will continue the development of an on-line fiber
optics toxic analyzer
  A manual  containing five standardized, short-
term chronic effluent toxicity test methods for
marine organisms will be published. Standardized
methods will be developed for specific human
pathogens such as Salmonella, Hepatitis A virus
and rotavirus in sludges, waters and wastewaters.
Additional reference toxicants and other biological
reference materials will be developed and
distributed
  The environmental processes and effects
program will continue  to develop, improve, simplify,
and test data bases and wasteload allocation
models to implement the water quality based
approach. The Center for Water Quality Modeling
will maintain and provide model codes, user
manuals, training and  user assistance  In addition,
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 work on the national atlas and maps of aquatic
 ecoregions will continue.
  Toxicity test methods for aquatic life will be
 developed, improved, verified, and transferred to
 Regions and States for predicting instream water
 body and biological impacts m fresh, brackish and
 marine systems. The significance of toxicity
 persistence to biota will be determined and
 methods developed for factoring these into the
 permitting process. Research will continue to
 integrate pollutant-specific toxic control techniques
 with whole effluent toxicity testing procedures and
 best available technology (BAT) limits for use in
 water quality permitting. Freshwater and marine
 specific chemical aquatic life criteria and advisories
 will be developed as needed. The research program
 will develop, modify, and apply experimental
 knowledge-based expert systems for environmental
 assessment needs
  Research will be conducted to strengthen the
 scientific and technical data base to support the
 Agency's effort to reduce the loss or degradation of
 the wetlands. Improved methods will be developed
 to assess individual and cumulative impacts of
 wetland conversions as well as effective means of
 mitigating impacts.
  The cooperative ecological research with the
 People's Republic of China will continue in FY 1 987
 to address the impact of contaminants on
 freshwater organisms, and will be at the stage in
 which field verification of methodologies will be
 emphasized
ISSUE: Wastewater Treatment Technology
  This issue focuses on the identification and
evaluation of technologies to improve the reliability
and cost-effectiveness of municipal and industrial
wastewater treatment facilities This research
emphasizes the technical  area in support of the
development and implementation of regulations for
the management of sludge produced by waste
treatment plants Standardized analytical methods
and quality assurance practices are needed to
support these efforts.

1986 Program Accomplishments
  In the scientific assessment area, a quantitative
risk assessment for the land application/
distribution and marketing sludge disposal option
for pathogens was  initiated using a revised version
of the Sandia Lab fate and transport model.
Qualitative pathogen  risk assessments for sludge
landfilling and ocean  disposal options were
completed.
  In the engineering area, major accomplishments
included five Regional seminars on evaluation of I/
A projects for the 100% modification/replacement
program, and the completion of eight I/A
technology evaluations The Process Design
Manual for Disinfection was published and
distributed at the October 1 986 WPCF conference.
Fiver Regional seminars on "Improving POTW
Performance Using the Composite Correction
Program Approach" were presented. Ten Design
Information Series reports were produced. Toxics
Interference Manual for Municipal Wastewater
Treatment was prepared in support of pretreatment
regulations  Assessment of the capability of six
alternative treatment technologies for the control of
toxics in marine waste discharges and a survey of
toxics reduction on six Ohio municipal wastewater
treatment plants were conducted. Reports were
completed on evaluations of enhanced chemical
oxygen demand (COD) removal in pharmaceutical
wastewater, pilot anaerobic biological treatment of
pulp mill evaporator foul condensates, evaluations
of granular carbon treatment of pesticide
manufacture wastewater, toxic reduction
evaluations at a multipurpose special chemical
plant with the development of a modified Walsh
fractionation procedure to identify toxics, and
alternatives to toxic organic paint strippers.
  The engineering research program continues to
support the development of sludge regulations.
Technical  support was provided to revise the draft
regulations on the pathogen content of sludge for
land disposal A paper entitled "Basis for Risk
Reference Dose for Dietary Cadmium Intake" was
published
  In the health effects area, research activities
were directed toward determining the health
effects of pathogens, organic and inorganic
chemicals in sludge. In addition, HERL has had
major input to ECAO's efforts to provide risk
assessment models for pathogens in sludge.
  A study of the pathogen content of D&M sludge
products was initiated in FY 1 986 to evaluate the
regulatory needs for the safe use of composted
sludge  Municipal sludges which are marketed or
otherwise distributed to the public as soil
amendments or fertilizer will  be analyzed for both
organic and inorganic  priority pollutants. Six
composite samples from each of two major cities
will be analyzed as well one composite sample from
each of 24 smaller cities. In addition to priority
pollutants, the mass spectra of the chromatograms
will be examined for other substances which occur
frequently or in high concentrations. A report on
this study  will  be completed by September 1 987.
  In addition, research results on the leaching of
viruses from sludge, survival of enteric viruses
during sludge storage, and determination of the
stability of viruses in sludge extracts were
published. These data will be useful in conducting
pathogen risk assessments.
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  A final report on the Lubbock Infection
Surveillance Study describing the relationship
between land application of wastewater and
infections in an exposed population was published
in 1986. The data from this study will be useful in
determining the risks of infectious disease to
humans living near wastewater land application
sites. The final report from the Ohio Farms Sludge
application study was completed, and three
separate sections reported on: (1) Health Effects; (2)
Tissue Metal Residues; and (3) Estimation of
Cadmium Intake.
  In the monitoring and quality assurance areas, a
report reviewing and updating the "Guidelines
Establishing Test Procedures for  Analysis of
Pollutants" was published in the Federal Register.
A report on "Systematic Approach to  Methods
Development for RCRA Appendix VIII Analytes"
was made at the Industrial Wastewater "Annual
Symposium on the Analysis  of Pollutants in the
Environment "

7557 Program Outlook
  In the scientific assessment area, identification of
five second round Hazard Indices/Environmental
Profiles  and one Pathogen Prototype Criteria
document for viruses will be initiated The scientific
assessment program will respond to the SAB and
public comments on land application chemical risk
assessment methodology. The pathogen land
application/distribution and marketing risk
assessment methodology and a bacteria prototype
document will be delivered to OWRS.
  In the engineering area, technology evaluations
in support of the 100% modification/replacement
program will be continued. The design, cost and
performance information will be  developed for
sludge stabilization and dewatermg processes, and
the evaluations of sludge management alternatives
Work  on improved design procedures and
parameters to correct design deficiencies and
improve performance and compliance of POTW will
be conducted toward the completion. Toxicity
reduction evaluation protocols and case histories,
and approaches to enhance  toxics removal in
wastewater treatment will be conducted Work will
be initiated on the engineering data base and
managerial techniques needed by states to apply a
system engineering approach in  implementing the
water quality control programs. Evaluations of
treatment technologies will  be continued to assist
the Industrial Technology Division in the
development of BAT limitations,  and on toxicity
reduction evaluations for use by  permitting
authorities  in writing best professional judgment
(BPJ)  NPDES permits. Work in toxics to be
conducted includes assessment  of treatment
mechanisms and the fate of toxics during
wastewater treatment, pilot study on acclimated
and un-acchmated wastewater, evaluation of
industrial wastewater treatment methods for the
removal of pharmaceutical chemical oxygen
demand (COD) in powder activated carbon
treatment (PACT) wastewater treatment, the
removal of phthalates from plastic molding and
forming wastewaters, and ammonium from
tungsten wastewater. A data base on the kinetics
of removal of toxics by sorption, volatilization, and
biodegradation will be developed. Methods to
facilitate evaluation and prediction of the
consequences of disposal of various wastes into
the freshwater and marine environment will be
evaluated and determined
  In FY 1 987, HERL plans to develop a computer
simulation method based on Kjellstrom and
Nordberg's kinetic model of cadmium metabolism
in the human being that will allow predicted
population frequency distributions of accumulated
renal cortex cadmium. Expected cadmium injection
levels resulting from various sludge usage
scenarios recently developed by HERL and MERL
staff will be used to predict the effect of the
increased cadmium in the food supply.
  A field study to determine the survival of Ascaris
ova in sludge amended soil will be completed in
early FY 1 987. Further studies on the infective dose
of enteric viruses will continue into mid-FY 1 988.
  The monitoring and quality assurance program
will review, update and distribute the annual report
on "Guidelines for Establishing Test Procedures for
the Analyses of Pollutants." Research to evaluate
and standardize HPLC/MS methods for sludge
matrices is planned. Plans to assist the regions in
evaluation and improvements of flow measurement
techniques and equipment are being made.
Supercritical fluid extraction techniques for field
preparation of samples will be investigated.
  Rapid, advanced techniques will be evaluated
and standardized to detect and monitor toxicity
entering municipal waste treatment plants and to
measure toxicity reduction during waste treatment
processes.
  Research will be conducted to identify and
determine the distribution of unlisted chemicals in
industrial wastewater. Compounds that can be
identified by empirical mass spectra matching, as
well as those that elude identification by this
technique, will be included


ISSUE: Marine, Estuaries and Lakes
  Marine research focuses on the development/
validation of protocols for predicting the impacts
from ocean disposal actions for use in the ocean
dumping permit program, the 301(h) waiver
program and the NPDES program. Technology-
related research in this area focuses on

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correlations between the type of treatment and the
resulting environmental impacts following disposal
and is expected to be used in assessing the
appropriate level of treatment for wastes to be
disposed to the ocean. Estuarine research develops
procedures and information that supports decisions
related to estuarine water quality. Great Lakes
research is conducted on the transport, fate and
effects of toxic materials in selected areas of that
ecosystem for use by the Great Lakes National
Program Offices, the Regions and the International
Joint Commission under the US/Canada Water
Quality Agreement.

1986 Program Accomplishments
  In support of EPA's ocean disposal activities,
effects and exposure methods were evaluated.
Emphasis was given to the development of a
wasteload allocation  model for municipal sewage
sludge at the 106-mile ocean dumping site. The
correlation of laboratory and field bioaccumulation
data for pollutants from dredged material was
completed. Researchers supported the 301 (h)
waiver program for ocean outfall discharges by
providing technical assistance, as part of a national
task force, for several applicants nationwide.
Methodologies were developed/evaluated for use
in predicting and assessing the effects of emission
products from the at-sea incineration  of hazardous
wastes. An inter-laboratory comparison of toxicity
from selected drilling fluids was conducted. A
report on the desorption of toxic metals from sludge
to marine waters was also completed.
  EPA's Great Lakes program was supported
through the development of a documentation
manual  for a phytoplankton simulation model,
completion of a study of biological effects along a
chemical gradient, and the development of a
source-fate-transport-exposure toxicity model for a
Great Lakes area of concern (Raisin River). A high
level of technical assistance was also provided to
the Great Lakes National Program Office, the
Regions end the International Joint Commission.

7357 Program Outlook
  The marine research program will continue to
develop  and test assessment procedures for
evaluating the impact of ocean dumping and ocean
outfall discharge of wastes, to develop and test
monitoring methods for coastal and deepwater
application, to develop and revise  screening
procedures for characterizing the bioaccumulation
potential of contaminants associated with wastes
to be ocean disposed, and to evaluate  the
significance of bioaccumulation processes,
resultant tissue residues and biological effects. The
program will also continue to evaluate the impacts
of drilling fluids and produced waters  on the marine
 environment. Research to develop a procedure for
 evaluating alternative technology options and their
 impact on the viability of ocean dumping will
 continue. Technology related research in this area
 will focus on the desorption of toxic organics from
 sludges to marine waters and on the fate of toxic
 organics and metals during treatment.
  The estuarine research program will be
 developing generic procedures for conducting
 wasteload allocations in estuaries. These
 procedures will be used to help make better source
 control decisions in the NPDES and construction
 grants programs.
  Great Lakes research will study the transport,
 fate and effects of toxics. Emphasis will be given to
 problems related to in-place pollutants. In additon,
 substantial technical assistance will continue to be
 given to the Office of Water, the Great Lakes
 National Program Office, and the Regions.
  The Office of Water is developing a Near Coastal
 Waters Strategy. The goal of this strategy is to
 maintain, and where possible, enhance
 environmental quality of near coastal waters. A
 research plan will be developed which is
 responsive to the needs expressed in the strategy.

 ISSUE:  Health Effects of Drinking Water
         Contaminants
  This research defines the health risks from
 exposure to drinking water contaminants. Areas of
 major concern deal with developing toxicological
testing and risk assessment methodologies for
complex mixtures, determining the public health
 risk from exposure to waterborne infectious disease
agents, conducting epidemiological studies to
determine health risk in human populations
exposed to various drinking water contaminants,
and providing criteria documents summarizing the
 relevant scientific data to support the health risk
assessment.

 1986 Program Accomplishments
  In FY 1986, 1 0 and/or 90 day toxicity studies
were completed for the following compounds:
tetrahydrofuran; 1,2-dichloropropane; 1,2,3-
trichloropropane; ethylene glycol; chlorobenzene;
 1,3-dichlorobenzene; and 1,2-dichloroethane. The
data from these studies will be used by ODW to
develop  Maximum Contaminant Level Goals
(MCLG) in support of drinking water standards.
  Trichloroethylene (TCE) has been reported to
increase the incidence of liver cancer in mice, but
not rats. Evidence suggests that the species
sensitivity to TCE resides in its ability to induce
peroxisome proliferation in mice, but not in rats.
Mice were shown to be sensitive to
tnchloroethylene and trichloroacetic acid (a by-
product of TCE metabolism) induction of
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peroxisome proliferation while rats were
insensitive. Both species were sensitive to
dichloroacetic acid, while monochloroacetic was
inactive in either species. Cytotoxicity studies using
primary cultures of rat and mouse hepatocytes
reflected the sensitivity to the TCE metabolites seen
for peroxisome proliferation induction in vivo.
These data support the idea that mouse liver
sensitivity to induction of peroxisome proliferation
by tnchloroacetic acid in part underlies the
carcinogenicity of trichloroethylene in that species.
  Chlorine dioxide and chlorite were evaluated for
effects on neurobehavioral development of rat
pups. Rat pups were indirectly exposed (via their
dam's drinking water) to various levels of these
compounds. The development of locomotor activity
of the rat pups was tested in a home cage
apparatus from 14 through 21 days of age. Pups
exposed to both compounds exhibited delays in the
development of locomotor activity when compared
to controls.
  A study to determine the carcinogenic and tumor
promoting activity of dichloroacetic acid,
trichloroacetic acid and trichloroethylene
administered  in the drinking water to B6C3F1 mice
was completed. The results indicate that both
dichloroacetic acid and trichloroacetic acid are
hepatocarcmogens. Not only are these chlorinated
acetic acids contaminants of drinking water, but
trichloroacetic acid is a major metabolite of
trichloroethylene and perchloroethylene. Therefore,
our results might also explain the
hepatocarcmogenic activity of trichloroethylene and
perchloroethylene, which are industrial
contaminants of ground and drinking water.
  The study of health effects associated with  point-
of-use granular activated carbon (GAG) filters in the
home is nearing  completion. Preliminary results
from that portion of the study dealing with by-pass
GAG home filters indicate that  there is no excess
illness or infection in a study group who used this
type  of filter relative to a group that did not use
GAG filters. The data from this  portion of the study
were very similar to that observed with faucet type
GAG filters, where no excess illness or infections
were linked to filter use.
  A two-day training session detailing Giardia
Immunofluorescent Antibody techniques was
conducted. Representatives from  Regions I and X,
the Pennsylvania Department of Environmental
Resources and the University of Pittsburgh
attended the session.
  The re-evaluation of the Aluminum Drinking
Water Criteria Document (DWCD) initiated some
minor revisions. Future action on this document
awaits direction from the Office of Drinking Water
(ODW). Public comments on all 28 Phase II
documents have been received and reviewed  and
 appropriate responses have been delivered to
 ODW.
  Three Phase IV drinking water criteria documents
 on disinfectants and disinfectant by-products were
 externally peer reviewed (chlorine and by-products;
 chloramines and ammonia, chlorophenols). A
 workshop was conducted on all eight of the Phase
 IV documents. The eight Phase V documents have
 been revised and prepared for external  review. The
 30 health advisories for Section 1445 unregulated
 VOCs were revised to incorporate comments
 received from  OHEAand internal ECAO-CIN
 reviews (rough external review drafts).

 1987 Program Outlook
  In FY 1987, the health effects program will
conduct 10 and 90 day subchronic toxicity studies
on: 1,2-dichlorobenzene, epichlorohydrin, 1,1,1-
trichloropropanone, chloropicrm, monochloramine,
chlorine, monochloroacetic acid, 2,4,6-
trichlorophenol, and 90 day studies on dichloro-
and trichloroacetic acids.
  In order to resolve the questions relating
disinfection of  drinking water to cardiovascular
disease, the following studies will be initiated: a
second pigeon study on the primary disinfectants to
independently  repeat the previous study, an animal
study on chlorine, chlorine dioxide, and  chloramine.
Human clinical studies on the disinfectants and
cardiovascular disease parameters will be
completed as well as the currently ongoing
epidemiology study.
  To determine the role of MX as a possible
mutagenic by-product of disinfection, in vivo tests
will be conducted to determine the  mutagenicity
and tumorgenicity of MX. This data will  be
important  in validating the results of in vitro studies
and will directly support development of an MCLG
for this disinfection by-product.
  The microbiology research program will conduct
a study to  determine the risk of infectious diseases
in populations  using point of entry drinking water
treatment devices. Infectivity data will be developed
for an additional  20 heterotrophic organisms
isolated from in home granular activated carbon
filters. A feasibility study will be conducted to
determine if a  population can be identified to
determine the  health significance of low levels of
viruses in  finished drinking water. Research on
identifying Giardia cysts in water supplies and
determining their viability and infectivity will
continue.
  Twenty-eight Phase II documents will undergo
quality assurance/quality control (QA/QC) checks
for submission as final documents. The  aluminum
document will  be finalized pending guidance  from
ODW. Phase IV documents will incorporate all
external review comments (Public/Workshop).
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 Phase V documents will undergo external review
 and final drafts will be prepared. Health advisories
 will be prepared as final External Review Drafts.
 ISSUE: Drinking Water Technology
   The Safe Drinking Water Act of 1 974 directs the
 Environmental Protection Agency (EPA) to set
 national drinking water standards. This research
 program is focused on developing a defensible
 basis for standards that apply to public water
 supply systems and providing technical assistance
 to states, regions and utilities.
   Major engineering research issues deal with the
 treatment of trace  organics and disinfection by-
 products. Also, control of microbiological
 contaminants and  development of a fundamental
 understanding of disinfection kinetics remain a
 relatively high priority. For example, there is
 general concern over the occurrence of Giardiasis
 in the U.S. and a growing awareness of problems
 resulting from poorly operated treatment plants and
 deterioration of water quality in distribution
 systems. Finding cost-effective treatment processes
 to remove regulated contaminants from small
 water supplies that are not in compliance is also
 receiving major attention.
   In the analytical  methods and mandatory quality
 assurance areas, 10 regional laboratories are
 evaluated annually in support of the National
 Interim Primary  Drinking Water Regulations
 monitoring certification program. This program is
 also responsible for methods development and
 analytical procedures to produce  precise and
 accurate total measurements systems for chemical,
 microbiological,  and radiochemical analysis.
 Technically and  economically feasible analytical
 procedures to be used by the Agency, states,
 municipalities and operators of public drinking
 water systems to monitor contaminants are also
 provided.

 FY 1986 Accomplishments
  A report on the cost and performance for
treatment of synthetic organic chemicals was
produced to assist Phase II regulations. Evaluation
of mplant trihalomethane control  technology was
completed to provide cost and performance
information. An interim report on  the inactivation of
microbial agents by chemical disinfectants was
produced. Evaluations of silicate and phosphate
compounds for their ability to inhibit corrosion of
different pipes were completed.
  About 1 5 final  reports were produced to provide
information on such topics as trihalomethane
control techniques, removal of radium from
drinking water, and the control of dioxins from a
granular activated carbon regeneration furnace.
About 25 presentations were made at national and
international meetings on EPA research activities
in VOC removal techniques, distribution system
problems, virus and bacteria  inactivation by
disinfection, and the effective operation of water
filtration plants.
  In support of the revised Primary Drinking Water
Regulations, analytical "Methods for the
Determination of Organic Compounds in Finished
Drinking Water and Raw Source Water" were
developed. These include packed wide-bore and
capillary GC and GC/MS methods for the nine
volatile organic chemicals (VOCs) and the 51
Section 1445 VOCs requiring monitoring. A method
employing the wide-bore column and the
photoionization and electrolytic conductivity
detectors in series is being evaluated for
determination of all 60 compounds. The laboratory
continued to support the National Inorganics and
Radiochemical Survey and is developing analytical
methods for the National Pesticides Survey.
  Drinking water certification training courses
were  offered to regional and  state  personnel in
chemistry and microbiology. On-site laboratory
evaluations were made in response to regional
requests.
  The Presence-Absence test, a rapid, inexpensive
method for detecting coliform bacteria in finished
drinking water, was evaluated. State-of-the-art
reports were prepared for methods for several
microbiological parameters under consideration for
proposed regulation of maximum contaminant
levels.
FY 1987 Program
  Research will continue to focus on developing
information to assist utilities in complying with
existing regulations and providing data to support
proposed regulations. Large-scale experimental
treatment technology projects will be conducted for
organic and inorganic contaminant removal,
especially related to ground water. Studies to
evaluate the effectiveness of chlorine and
alternative disinfectants for the control of
waterborne pathogens will be continued.
Evaluations of treatment technology for
radionuclide removal and residues disposal
management will also continue. Studies to
determine the factors leading to deterioration of
water quality in distribution systems will be
conducted, and problems of small systems,
including system design, performance and
operation criteria, and institutional and pricing
policies will also be studied. Field scale evaluations
of granular activated carbon (GAC) technology and
additional treatment technology data for new
chemicals under the Safe Drinking Water Act

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Amendments will be initiated in support of
regulation developments.
  The monitoring and quality assurance program
will provide methods development and analytical
procedures to produce precise and total
measurement systems for chemical, radiochemical,
and microbiological analysis and will develop less
expensive methods for new parameters including
synthetic organic chemicals (SOCs) which have
been established. Final standardized methods for
these SOCs and the pesticides for the underground
water survey will be published. The program will
also conduct laboratory certification for the 10
regions for organic and inorganic chemicals and
microbiological analysis, will produce and distribute
quality assurance and performance evaluation
samples and standard reference  materials, and will
also conduct requested on-site laboratory
evaluations for radiochemicals analysis of drinking
water.
  Research will begin on the development and
standardization of rapid, inexpensive new methods,
such as gene probes and monoclonal and
polyclonal antibody methods for detecting and
quantifying pathogen  microorganisms in water
supplies and finished  drinking water.
ISSUE: Ground Water
  Our science for assessing and predicting the
impacts of ground water pollution is meager but
growing. In the past few years, important gains
have been made by the EPA ground water research
program in technology for accessing the subsurface
and taking samples that are contaminated by the
sampling process. Further, we know reasonably
well how a few organic chemicals of concern
behave in a few geological materials. However, the
state-of-the-art for ground water monitoring is
cumbersome, expensive, and  insufficiently precise.
Our capability for predicting the behavior of organic
and microbiological contaminants is limited. Finally,
there is little information available on the
effectiveness for the costs of methods for in-situ
cleanup of already polluted aquifers.
1986 Program Accomplishments
  A number of significant scientific advances were
reported. "Aerobic Degradation of Halogenated
Methanes, Ethanes, and  Ethylenes by a Natural
Gas-Stimulated Microbial Community" reported the
discovery of a method by which trichloroethylene
and other important ground water contaminants
may be degraded in place. "Behavior of the
Hepatitis A Virus in Subsurface Systems" and
"Predictive Models for Pathogens in Ground
Water" described our increasing knowledge about
potential contamination of underground water
supplies from surface sources. "Physical and
Chemical Components of Dispersion" provided
important new information about a significant
problem in ground-water contaminant models.
  Two major reports were published: EPA Ground-
Water Research Programs, and Ground-Water
Quality Protection—State and Local Strategies.
  Training efforts continued with basic ground-
water hydrogeology courses taught at five EPA
Regions, and the publication of a Training Manual
on Ground Water Contaminant Modeling. Other
important information transfer efforts also
continued with  support of the International
Ground-Water Modeling Center and the National
Ground Water Information Centers.
7557 Program Outlook
  The program will continue to focus on studies of
subsurface processes such as sorption,
biotransformation, redox, hydrolysis, and ion
exchange. Research to evaluate the cost-
effectiveness of aquifer restoration methods, such
as the one described above for trichloroethylene,
will also continue.
  Major outputs are expected to support the
current regulatory emphasis on determining the
safety of deep-well injection of wastes. Research  is
underway on fluid front movement from Hazardous
Waste injection wells, interaction of injected fluids
with injection zone materials, and mechanical
integrity test methods.
  Emphasis will also continue on technical
assistance and information transfer Training of
Regions is expected to continue for several years,
and support of the International Ground-Water
Modeling Center and  the National Ground Water
Information Center will also continued.
  Research resulting  from the wellhead protection
provision of the Safe Drinking Water Act
Amendment is being proposed.
  In the monitoring and quality assurance area, the
program will continue to support the Regions in
locating abandoned wells. Research will continue
to determine the application of geophysics and
other techniques for detecting and mapping fluid
movement from injection wells. Evaluation and
development of laser-induced fluorescence for
monitoring ground-water contamination using fiber
optics will also continue. Hollow stem auger
methods will be evaluated to determine if sampling
wells completed by this method contribute to
vertical movement of contamination outside well
casings, potentially contaminating potable ground
water.
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Environmental Results and Projected
Output

ISSUE: Water Quality Based Approach/
        Permitting

FY 1986 Results

•  Report on Evaluation of Metals Dissolution for
   Chromium.

•  User-Friendly IBM PC Computer Programs for
   Solving Sampling and Statistical Problems.

•  Development of Methods for Collection and
   Analysis of Precipitation.

•  Report on Methods for Distinguishing Compounds
   of Mercury.

•  Third Quarter/Annual Alternative Test Procedure
   (ATP) Application Report.

•  Equivalency Applications Received, Notice and
   Comments Published in Federal Register, Final
   Notice Published in Federal Register.

•  Methods for Selected Synthetic Organic
   Compounds—Summary Report.

•  Evaluation of Method 200.1, Determination of
   Acid Soluble Metals.

•  Recommendations to the Office of Drinking Water
   on Analytical Procedures for Virus Monitoring of
   Drinking Water.

•  Status Report on "Virus Recoveries from
   Retrofitted Aerobic Sludge Digesters," submitted
   to Wastewater Research Division, Water
   Engineering Research Laboratory, Cincinnati.

•  Report to Wastewater Research Division, Water
   Engineering Research Laboratory, Cincinnati, on
   indigenous viruses isolated from sludge
   digesters undergoing different feeding protocols.

•  Annual Report on the Repository for Toxic and
   Hazardous Materials.

•  Annual Report on Quality Control  Samples
   Program.

• Annual  Report on Referee Analytical Services.

•  Annual Report on the Traceability to the National
   Bureau of Standards.

•  "Methods for Synthetic Organic Compounds
   (SOCs)."
•  Report on analytical components of field study to
   test WLA models.

•  TOXIWASP Workshop—Annapolis, MD.

•  QUAL—lie Workshop—Boston, MA

•  User's manual for metals including
   enhancements to MEXAMS with tutorial.

•  Map of aquatic ecoregions of the conterminous
   United States.

•  Journal article on methods to measure
   bioaccumulation resulting from sediment
   toxicants.

•  Report on case studies on toxicity tests of complex
   effluents to predict aquatic community impacts-
   Ohio River, Wheeling, WV, Skeleton Creek, Enid,
   OK, Naugatuck River, Waterbury, CT, and
   Kanawha River, Charleston, WV

•  Report on feasibility of toxicity factors model to
   derive site-specific criteria.

•  Journal article on field evaluation of site-specific
   criteria for ammonia.

•  Ambient Water Quality Criteria for Nickel,
   Dursban, Pentachlorophenol, Dissolved Oxygen,
   Toxaphene, Selenium and Zinc.


Projected FY 1987 Outputs

•  Delivery of 10 Water Quality Advisors (human
   health portions) to the regions on unregulated
   chemicals found  in ambient water.

•  Preparation of 10 human health documents of
   Ambient Water Quality Criteria on presently
   unregulated chemicals.

•  Review of up to four 301 (g) CWA, variance
   requests for program office acceptance process.

•  Response to technical assistance as requested.

•  Quality Assurance  Support of Regional, State,
   Municipal Monitoring by Preparation and
   Distribution of Calibration Materials.

•  Evaluation and Validation/Standardization of
   Monitoring Methods for Monitoring Methods:
   Chemical, Biological, and Physical.

•  Development/Improvement to Monitoring
   Methods: Analysis of Sludge and Wastewater
   Contaminated by Non-Gas Chromatographic/
   Mass  Spectrometer Analyzable Pollutants (Dye
   Formulator).
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• Health Effects Bioassay Methods Manual for
  Determining Whether Receiving Streams Meet
  Water Quality Standards.

• Report on framework for application of
  ecoregional approach to water quality standards
  setting.

• Report on the relationship between lipid content,
  body burden, and thermodynamics calculation of
  bioaccumulation.

• Report on updated documentation for QUAL-lle
  including uncertainty analysis.

• Training courses, through the Center for Water
  Quality Modeling, including QUAL-lle, WASP
  4.1, EXAMS Hand CYNTOX.

• Journal publication and guidance relative to
  toxicity persistence

• Report on the feasibility of using complex effluent
  toxicity to allocate wasteload at an estuarme
  site.

• Report on toxicity characterization at a field site.

• Journal article on the life cycle toxicity of Al, low
  ca, and low pH to fathead minnows.

• Report on use of toxicity factors for Copper
  Criteria.

• Ambient Water Quality Criteria for 10 compounds.

• Ambient Water Quality Advisories for  10
  compounds.

• Journal article describing algorithms for
  estimating photochemical rate constants from
  chemical properties.

• Report on design of a decision support system for
  freshwater wetlands.

• Report on experimental approach to testing of
  mitigation projects.

• Process-based ecosystem model of water quality
  functions of freshwater wetlands.

• Report on approach for assessing the cumulative
  impacts of wetland loss.

• Internal report on application of water quality
  criteria and effluent toxicity tests in the control
  of toxic chemicals in China.

• Internal report on application of models for
  conventional and toxic pollution  assessment in
  China.
• Internal report on joint US-China studies on
  pollutant transformation processes.

ISSUE: Wastewater Treatment Technology

FY 1986 Results

• Initiation of a quantitative risk assessment for the
  land application/distribution and marketing
  sludge disposal option for pathogens.

• Completion of qualitative pathogen risk
  assessments for sludge landfilling.

• Completion of qualitative pathogen risk
  assessments for sludge ocean disposal options.

• Regional seminars on evaluation of I/A projects
  for the 100% modification/replacement
  program

• Evaluation reports on eight I/A technologies.

• Process design manual for wastewater
  disinfection.

• Regional seminars on "Improving POTW
  Performance Using the Composite Correction
  Program Approach."

• Series reports on design-information.

• Toxics interference manual for municipal
  wastewater treatment.

• Assessment of the capability of six alternative
  treatment technologies for the control of toxics
  in marine waste discharges.

• A survey of toxics reduction of six Ohio municipal
  wastewater treatment plants.

• Report on evaluations of enhanced COD removal
  in pharmaceutical wastewater.

• Report on pilot anaerobic biological treatment of
  pulp mill evaporator foul condensates

• Report on evaluations of granular activated carbon
  treatment of pesticide manufacture wastewater

• Report on toxic reduction evaluations at a
  multipurpose special chemical plant with the
  development of a modified Walsh fractionation
  procedure to identify toxics.

• Technical support to revise the draft regulations
  on the pathogen content of sludge for land
  disposal.

•  Report on the current frequency  of occurrence of
  toxic pollutants in industrial wastewater.
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 • Report on the results of a feasibility study to
   determine the best tool for database
   management system (DBMS) construction.
 Projected FY 1987 Outputs

 • Revision/refinement of five environmental profile
   documents to be utilized by OWRS for screening
   of chemicals for possible regulation under CWA
   405(d).

 • Delivery of the pathogen land application/
   distribution and marketing risk assessment
   methodology for health-based criteria to OWRS.

 • Delivery of Bacteria Prototype Document for
   Deriving Criteria for Bacteria in Sludge to OWRS.

 • Report on the Occurrence of  Microorganisms in
   Distribution and Marketing Sludge Products.

 • Technology evaluations in support of the 100%
   modification/replacement program.

 • Report on the design, cost and performance
   information for sludge stabilization and
   dewatering  processes.

 • Evaluations of sludge management alternatives.

 • Improved design procedures  and parameters to
   correct design deficiencies and improve
   performance and compliance of POTW.

 • Toxicity reduction evaluation protocols and case
   histories, and approaches to  enhance toxics
   removal in wastewater treatment.

 • Evaluations of treatment technologies to assist the
   Industrial Technology Division in the
   development of BAT limitations.

 • Toxicity reduction evaluations for use in writing
   best professional judgment (BPJ) NPDES
   permits.

 • Reports on the removal of phthalates from plastic
   molding and forming wastewaters, and
   ammonium from tungsten wastewater.

•  Development of data base on kinetics of removal of
   toxics by sorption, volatilization, and
   biodegradation.

•  Report on performance of capillary column data
   using the VAX 785 computer.
 ISSUE: Marine, Estuaries and Lakes

 FY 1986 Results

 • Development of wasteload allocation model for
   municipal sewage sludge at the 1 06-mile ocean
   dumping site.

 • Correlation of  laboratory and field
   bioaccumulation data for pollution from dredged
   material.

 • Technical assistance in support of 301(h) waiver
   program for ocean outfall discharges, and to
   national and international offices with  Great
   Lakes concerns.

 • Development and evaluation of methods to assess
   effects of emission products from hazardous
   wastes incinerated at sea.

 • Inter-laboratory comparison of toxicity  from
   selected drilling fluids.

 • Development of source-fate-transport-exposure
   toxicity model  for Great Lakes area of concern—
   the River Raisin.

 Projected FY 1987 Outputs

 • Development and testing of procedures for
   evaluating impacts of ocean dumping/ocean
   outfall discharge of wastes.

 • Evaluation of significance of bioaccumulation
   processes, tissue residue, and biological effects
   of contaminants in wastes to be ocean-disposed.

 • Evaluation of impacts of drilling fluids on marine
   environments.

 • Development of procedures for conducting
   wasteload allocations in estuaries to support
   NPDES and construction grant programs.

 • Elucidation of  problems related to in-place
   pollutants in Great Lakes; technical assistance to
   national and international offices with  Great
   Lakes concerns.

 • Development of research plan to respond to Near
   Coastal Waters Strategy.
ISSUE: Health Effects Drinking Water
        Documents

FY 1986 Results

•  Preparation of 30 draft Drinking Water Health
   Advisories on unregulated VOCs.
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• Disposition of public comments received on Phase
  II DWCDs and Agency response document.

• Three final  draft documents of the Phase IV
  documents on disinfectants and disinfectant by-
  products.

• Eight external review drafts of Phase V chemicals.

• Report on the Percutaneous Absorption of
  Chemicals in Drinking Water.

• Published International Symposium on the Health
  Effects of Disinfectants and Disinfectant By-
  Products.

• Published a Review of the Occurrence and
  Significance of Viruses in Drinking Water.

• Published an Annual Summary of Waterborne
  Disease Outbreaks.

• Report on Parameters Governing the Volatilization
  of Trichloroethylene into Indoor Air from a
  Laboratory  Model Shower.

Projected FY 1987 Outputs

• Fmalization of 22 Phase II DWCD for MCLGs/
  MCLs proposal  1988.

• Incorporation of external review comments  and
  updating 1 6 Phase V documents for MCLGs/
  MCLs proposal  1989.

• Updating three  Phase IV DWCD on disinfectant
  and disinfectant by-products for FY-90
  regulation.

• Report on Cancer and Reproductive Hazards of
  Principal Contaminants in Drinking Water.

• Report on Target Organ Toxicity of Major Chemical
  Contaminants in Drinking Water.

• Report on Cardiovascular Disease Risk Factors m
  Populations Supplied with Chlorinated and
  Nonchlorinated Drinking Water of Varying
  Hardness.

• Determination of Virulence and Growth
  Conditions of Legionella.
 ISSUE: Drinking Water Technology

 FY 1986 Results

 • Provided and distributed 55,000 quality control
   and performance evaluation samples for
   chemical, radionuclide,  and microbiological
   analysis of drinking water.
• Conducted methods validation studies for organic,
  inorganic, and microbiological analysis of
  drinking water.

• Evaluated 10 regions and personnel for
  capabilities to provide quality data in support of
  the NIPDWR's monitoring (laboratory
  certification) program.

• Evaluated alternate test procedures for chemical,
  microbiological and radiochemical analysis of
  drinking water.

• Report on the treatment of synthetic organic
  chemicals in  drinking water.

• Report on the mactivation of microbial agents by
  chemical disinfectants.

• Report on investigation of treatment modifications
  other than the use of alternate disinfectants for
  the control of trihalomethanes.

• Report on investigation of design scale-up
  suitability for air stripping columns for the
  removal of volatile organic chemicals from
  groundwater.

• A report on the evaluation of the presence of dioxin
  and furans in all effluent streams from the
  fluidized-bed GAC reactivation furnace.
Projected 1987 Outputs

• Provide methods development and analytical
  procedures to produce precise and accurate total
  measurement systems for chemical,
  radiochemical and microbiological analysis.

• Develop and distribute quality control (QC) and
  performance evaluation (PE) samples for drinking
  water laboratory certification and evaluation
  programs.

• Conduct laboratory evaluation for 10 regions for
  laboratory certification program.

• Evaluate alternate test procedures for drinking
  water analysis.

• Overview Agencywide mandatory quality
  assurance program for drinking water.

• Report on removal of radium from drinking water.

• Report on removal or prevention of disinfection by-
  products and precursors.

• Report on concentration times time (Cxt) values for
  Hepatitis A and coliphage inactivation.
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   Report on carbon usage rate data for determining
   the cost of removal of specific organics on Phase
   II Regulatory calendar.

   Report on carbon usage rate data for determining
   the cost of removal of specific organics on Phase
   V Regulatory calendar.

   Report on inexpensive low-tech removal methods
   for radon.

   Report on performance and cost information on a
   full-scale ion exchange plant for nitrate removal.

   Report on field evaluation of packed tower
   aeration for the removal of volatile organic
   contaminants from groundwater.

   Report on the performance of air stripping and
   granular activated carbon for removal of volatile
   organic contaminants from groundwater.
ISSUE: Ground Water

FY 1986 Results

•  Evaluating initial methods for constructing
   monitoring wells with hollow stem augers.

•  Investigating use of fiber optics for monitoring
   contaminants in groundwater.

•  Annual report on source variability affecting
   ground-water monitoring.

•  Described a method by which trichloroethylene
   and other important ground-water contaminants
   may be degraded in the subsurface.

•  Described how a number of viruses survive and
   move in ground water.

•  Provided new evidence on the nature of
   dispersion, the most important problem in
   ground-water contaminant transport modeling.

•  Provided a description of important strategies for
   State and local ground-water protection
   programs.

•  Provided a consolidated description of current
   ground-water research.

•  Provided materials and conducted training in
  ground-water modeling.

•  Continued other important information transfer
  efforts, including the International Ground Water
   Modeling Center and the National Ground Water
   Information Center.
Projected FY 1987 Outputs

• Report on the use of hollow stem augers for
  constructing monitoring wells.

• Report on developing methods for mapping fluid
  movement from injection wells.

• Field testing techniques for locating abandoned
  wells.

• Report on development of fiber optics for
  monitoring ground-water contaminants.

• Computer code and documentation for predicting
  organic chemical solubility in mixed solvent
  systems.

• Article on adaptation of subsurface microbes to
  degrade organic pollutants.

• Report on study of Class I  injection wells.

• Report on cement bonding of injection wells.

• Interim report on the use of ground-water models
  in wellhead protection.
  US GOVERNMENT PRINTING OFFICE 1987 748-121/40724
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