United States
                          Environmental Protection
                          Agency
Office of Acid Deposition,
Environmental Monitoring and
Quality Assurance
Washington DC 20460
EPA/600/M-90/001
   April 1990
                          Research and Development
&EPA      AERP   status
                                                                                           Eooia
                                                                            I
                             5U604
                          The Aquatic Effects Research Program (AERP) status provides information on AEF
                          projects  dealing with the effects of  acidic  deposition on  U.S.  surface waters.
                          Our objectives are to:

                            •   assist organizations involved in acidic deposition research to avoid duplicator
                                of efforts and to make maximum use of related research,

                            •   promote communication among the Environmental Protection Agency (EPA),
                                state agencies, and organizations involved in acidic deposition monitoring
                                activities, and

                            •   provide a mechanism to distribute available AERP information.

                          AQUATIC EFFECTS RESEARCH PROGRAM-AN  OVERVIEW	

                          In 1980, Congress passed the Acid Precipitation Act, thus establishing the
                          Interagency Task Force on Acid Precipitation.  Given a 10-year mandate, the Task
                          Force implemented the National Acid Precipitation Assessment Program (NAPAP) to
                          investigate the causes and effects of acidic deposition.  NAPAP includes task groups
                          formed to study emissions and controls, atmospheric chemistry, atmospheric
                          transport, atmospheric deposition and air quality, terrestrial effects, aquatic effects,
                          effects on materials  and cultural resources, and direct and indirect human health
                          effects.

                          The AERP, formed in 1983  as part of the NAPAP Aquatic Effects Task Group, is
                          responsible for assessing  the effects of acidic  deposition on aquatic ecosystems.
                          Already, published AERP reports have described the chemical characteristics of lake
                          and stream resources in regions of the United States potentially sensitive to acidic
                          deposition. Complementing these  findings, a report to be published in  early 1990
                          summarizes correlative relationships between watershed and surface water chemica
                          characteristics and projects future conditions for two deposition scenarios in the
                          Northeast and two in the Southern Blue  Ridge Province.  (See Direct/Delayed
                          Response Project article, page 5. For a  complete list of published AERP documents
                          see the mail order form attached to this status.)

                          By 1990, the end of the 10-year mandate, Congress requires NAPAP to provide a full
                          assessment of the acidic deposition phenomenon.  An important aspect of current
                          AERP efforts involves synthesizing results from past and current research to
                          describe the state of the science for acidic deposition effects on aquatic systems.
                          Another aspect involves integrating the state-of-science information with illustrative
                          emission control scenarios to provide an assessment useful for policy decisions
                          concerning alternative control  strategies.  A group of AERP scientists is now workin
                          on this task, which will provide valuable  aquatic information for the NAPAP reports
                          Congress.  A summary of  these activities can be found on page 9.

                          Status of AERP Activities-This issue of the status includes sections that provide
                          information about  recently published AERP materials and projects in progress.
                          Table 1 summarizes the present status of projects within the AERP.

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                                                AERP status
Project
National Surface Water Survey
National Lake Survey-Phase I (East and West)
National Lake Survey-Phase II (Northeast)
National Stream Survey-Phase I
Direct/Delayed Response Project
Northeast and Southern Blue Ridge Province
Mid-Appalachian Region
Watershed Processes and Manipulations
Watershed Manipulation Project
Watershed Recovery Project
Little Rock Lake Experimental Acidification Project
Episodic Response Project
Episodes
Regional Episodic and Acidic Manipulations Project
Temporally Integrated Monitoring of Ecosystems
Biologically Relevant Chemistry
Indirect Human Health Effects
Design

Complete
Complete
Complete

Complete
Complete

Complete
Complete
Ongoing

Complete
Complete
Ongoing
Ongoing
Complete
Implementation

Complete
Complete
Complete

Complete
Ongoing

Ongoing
Ongoing
Ongoing

Ongoing
Ongoing
1991
Ongoing
Complete
Reporting

Complete
1990
Complete

Complete
Fall 1990

Dec. 1992
Fall 1990
Annually

1990/1991
Summer 1990
Annually
Summer 1990
Fall 1990
 Table 1. Present status and projected dates for stages of major AERP projects.
AERP FEATURE ARTICLE
Long-Term Monitoring (LTM) and Temporally
Integrated Monitoring of Ecosystems (TIME)
Projects

Long-term monitoring of surface water resources
plays a  pivotal role in the assessment of acidic
deposition effects. The Long-Term Monitoring (LTM)
and Temporally Integrated Monitoring of Ecosystems
(TIME) projects represent two responses by the AERP
to establish a data base  on trends in aquatic effects
of acidic deposition on acid-sensitive surface waters.

LTM  was initiated in 1983, prior to initiation of the
National Surface Water Survey (NSWS) and the
establishment of the AERP.  LTM incorporated sites
from other ongoing or planned monitoring networks
involved in studies of acid-sensitive surface waters
across a regional and national range of acidic
deposition. The LTM network, whose current
configuration is shown in Figure 1, is composed of 85
sites in  six regions of  the United States. Samples for
chemical analysis are collected and analyzed on a
monthly or seasonal schedule by individual
cooperators (Table 2).  The quality assurance program
is coordinated at the EPA Environmental Research
Laboratory in Corvallis, Oregon and includes
participation in an international round-robin, natural
audit program coordinated by the Canadian Long-
Range Transport of Atmospheric Pollutants  (LRTAP)
program.  A description of the  1985 configuration of
the LTM project is given in Newell et al. (Analysis of
Data from Long- Term Monitoring of Lakes, EPA/600/4-
87/014; to obtain copies of this report and others
mentioned in this article,  contact the  Technical
Director listed at the end of this article.)  Despite the
short data record (4 to 7 years),  some trends in water
quality at LTM sites have been detected (Figure 2),
including decreases in sulfate concentrations in the
Northeast and decreases in acid neutralizing capacity
(ANC) in some of the New York and Upper Midwest
sites.  Sediment cores for paleolimnological
examination have recently been collected  from many
LTM lakes. These cores  will help corroborate recent
trends observed in these lakes and put recent
changes into the perspective of long-term (>100
years) trends.
                                                    -2-

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                                                  AERP status
   LTM  SITES IN COLORADO
    deposition ca. 0.67
Figure 1.   Location of Long-Term Monitoring (LTM) sites in Maine, Vermont, New York, Colorado (inset), and the Upper Midwest. Isopleths
         of sulfate wet deposition (g/m */yr, 1980-84) are overlaid to indicate the range in deposition that these sites experience.
The foundation for many of the AERP projects is the
use of randomly selected probability sample sites to
provide population estimates on a regional basis.

Since the LTM sites were not selected statistically, the
questions of how or whether results from this
network could be used in regional assessments gave
rise to the TIME project.  TIME was designed with
four goals in mind:  (1) to provide regional early
warning signals of surface water acidification or
recovery; (2) to provide an ongoing assessment of
regional patterns or trends in surface water
acidification or recovery; (3) to assess the extent to
which observed spatial and temporal patterns in
surface water chemistry correspond with model
forecasts; and (4) to assess the  relationships
between patterns in surface water chemistry and
patterns in atmospheric deposition.  The current TIME
design combines  temporally intensive monitoring at a
small number of trend sites in each region with
spatially extensive, regional resurveys of sites selected
from a statistical population frame.

Planning for the TIME project has emphasized the
need to evaluate statistical techniques for data
analysis as part of the design process to ensure that
the final design will produce data that specifically
address the four goals of TIME.  Planning activities
have included:

   •    Evaluations of over a dozen statistical
        techniques, both univariate and multivariate,
        for trend detection  in time series data.
        Seasonal Kendall tests, and analysis of
        covariance on ranked data, were selected as
        the trend detection  methods of choice
        (discussed further in Loftis et al., An
        Evaluation of Trend Detection Techniques for
        Use in Water Quality Monitoring Programs,
        EPA/600/3-89/037).

   •    Reviews  of the  potential for using biological
        indicators in the assessment of surface wate
        acidification.  Successful monitoring plans for
        fish, amphibians, zooplankton, algae
        (periphyton, chrysophytes, dinoflaggelates,
        diatoms, greens and bluegreens), and
        macroinvertebrates will be presented in an
                                                      -3-

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                                                  AERP status
Location
Adirondacks
Vermont
Maine
Upper Midwest
Rocky Mountains
Catskill
Mountains
Principal
Invest igator(s)
Charles Driscoll
Wallace McLean
Doug Burnham
Jim Kellogg
Terry Haines
Steve Kahl
Patrick Brezonik
Katherine Webster
John Turk
Pete Murdoch
Affiliation
Syracuse University
Vermont Agency
of Environmental
Conservation
U.S. Fish and Wild-
life Service; University
of Maine
University of
Minnesota; Wiscon-
sin Department of
Natural Resources
U.S. Geological
Survey, Denver
U.S. Geological
Survey, Albany
Number
of Sites
15 lakes
24 lakes
5 lakes
27 lakes"
10 lakes
4 streams
Start
Spring
1985
Winter
1981
Fall
1982
Fall
1983
Summer
1985
Fall
1983
Sampling
Schedule
Monthly
4 seasons
per year
3 seasons per
year
3 seasons per
year
Monthly in
summer
9 times
per year
Comments
Historical data are
available (sampled monthly,
from 1982-1984)".
Changes in sampling and
analytical methods were
made in spring 1985 which
may affect trend
interpretation.

Historical data are
available (sampled
sporadically on a seasonal
basis, from 1978 to 1983)c.

Sampling is flow
directed.
a Data collected by Syracuse University for most of these lakes between 1982 and 1985 were funded by the Electric Power Research
  Institute under the Regional Integrated Lake Watershed Acidification Study.

b Four lakes in Minnesota; 12 lakes in Wisconsin; 11 lakes in Michigan.

c Data collected by the University of  Minnesota-Duluth and the Wisconsin Department of Natural Resources through a cooperative
  agreement with the Environmental Research Laboratory-Duluth are available for most of these lakes for the period 1978-1983. The
  sampling schedule for these lakes was variable, ranging from twice a year to seasonally, and included multiple samples per lake.


 Table 2.  EPA Long- Term Monitoring Project.
     upcoming EPA report, The Role of Biomonitoring
     in Assessing the Aquatic Effects of Acidic
     Deposition.

  •  Multivariate analyses of NSWS data sets and
     development of conceptual models which will
     allow correlation of nonrandomly selected sites
     (as in LTM) to  probability samples such  as the
     NSWS and the regional resurveys planned for
     TIME.

Other areas identified for development have included
assessing the adequacy of existing deposition
networks for the TIME objectives, developing
statistical techniques to relate patterns  and trends in
one monitoring network (e.g., deposition) to another
network with different spatial and temporal resolution
(e.g., surface waters), developing statistical
techniques for analysis of biological data, and
developing an approach for evaluating model
forecasts of surface water acidification.  Several
papers on these topics will appear  in the Proceedings
of an International Symposium on the Design of
Water Quality Information Systems (in press, to be
published by Colorado State University).  In 1989, the
TIME and LTM projects were reassigned from the
AERP to the EPA Environmental Monitoring and
Assessment Program (EMAP).   EMAP has the broad
mission to provide  periodic descriptions of general
ecosystem health across the United States.  Thus,
EMAP treats  several different target resources (near
coastal environments, forests, wetlands, agroeco-
systems, deserts, grasslands, tundra, and air) in
addition to surface waters. EMAP intends to monitor
a range of stressor indicators  (unlike current
monitoring aimed specifially at acidic deposition), and
a range of exposure and response indicators, with a
particular emphasis on biological responses.  The
EMAP design is a tiered, hierarchical approach whose
foundation is an evenly spaced sampling grid of about
12,500 points.  For  more information about EMAP
contact:

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                                                 AERP status
                                              LTM  LAKES
                                          Maine, New York, Upper Midwest
  cr
   0.10) are shown with slopes of zero.
  Jay Messer
  EMAP Deputy Director
  EPA/Atmospheric Research and
   Exposure Assessment Laboratory
  Mail Drop 75
  Research Triangle Park, North Carolina 27711
  (919) 541-0150

One  of EMAP's goals is to provide assessments of
the effectiveness of regulatory programs, such as the
proposed amendments to the Clean Air Act.  Under
EMAP, the TIME and LTM projects will evolve to meet
this goal. The general EMAP design will be
augmented to  allow the monitoring of a specific
stressor (acidic deposition) and a set of regulatory
programs (emissions reductions).

For more information about the TIME  and LTM
projects, and to obtain copies of reports cited in this
article, contact:

  Jesse Ford
  EPA/Environmental Research Laboratory-Corvallis
  200 S.W. 35th Street
  Corvallis, Oregon 97333
  (503) 757-4666
  FTS: 420-4666
                                                        John L. Stoddard
                                                        TIME/LTM Technical Director
                                                        EPA/Environmental Research Laboratory-Corvallis
                                                        200 S.W. 35th Street
                                                        Corvallis, Oregon 97333
                                                        (503) 757-4666
                                                        FTS: 420-4666

                                                       CURRENT AERP ACTIVITIES	

                                                       Summarized below are the AERP activities that are
                                                       currently in progress.

                                                       Direct/Delayed Response Project (DDRP)

                                                       The Direct/Delayed Response Project (DDRP) was
                                                       designed to examine critical scientific and policy
                                                       questions regarding potential future acidification  [loss
                                                       of acid  neutralizing capacity (ANC)] in eastern
                                                       watersheds (April  1989 status).  The final report for
                                                       lakes in the Northeast and stream reaches of the
                                                       Southern Blue Ridge Province was released  in July
                                                       1989; major findings were reported in the July status.
                                                       The report, entitled Direct/Delayed Response Project:
                                                       Future Effects of Long- Term  Sulfur Deposition on
                                                       Surface Water Chemistry in the Northeast and
                                                       Southern Blue Ridge Province, Volumes I-IV
                                                       (EPA/600/3-89/061 a through d) will be available in earl
                                                       1990.
                                                     -5-

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                                                     AERP status
Data analyses continue for 36 DDRP watersheds
being studied in the Mid-Appalachian Region (October
1988 status), and results will be reported as part of
NAPAP 1990 Integrated Assessment.  These analyses
are focusing on the Level II (Single Factor Response
Time Estimates) and Level III (Integrated Watershed)
modeling activities analogous to those  performed for
the Northeast and Southern Blue Ridge Province
regions.  Only a single Level III watershed model-
Model  of Acidification of Groundwater in Catchments
(MAGIC)--is being used in the Mid-Appalachian
studies, because the three models used for the
Northeast and Southern Blue Ridge Province analyses
yielded similar results.

Correction to DDRP  article in September 1989
status:

Tables 2 and 3  on page 5 of the September issue of
the AERP status (EPA/600/M-89/022) contained a
number of erroneous entries with regard, principally,
to the  95 percent confidence estimates of the
projections of the DDRP.  The corrected values appear
in the accompanying tables  published here.  Figure 3
Number of Lakes8
Time from
Present
Year
0
20
50
Constant
ANC <0
162b
5%
161 (245)
5% (8%)
186 (251)
6% (8%)
Deposition
ANC <50
880b
27%
648 (319)
20% (10%)
648 (329)
20% (10%)
Decreased
ANC <0
162b
5%
136 (230)
4% (7%)
87 (237)
3% (7%)
Deposition
ANC <50
880b
27%
621 (313)
19% (10%)
586 (331)
18% (10%)
a % is percentage of the target population of 3,227 lakes; ( )
  indicate 95 percent confidence estimates.
b Indicates estimate from National Surface Water Survey-Phase I
  sample for the same 123 lakes; target population = 3,227
  lakes.
c Projections are based on 123 lake/watersheds successfully
  calibrated by MAGIC.
d See Figure 3 for definition of the deposition scenarios used.
 Table 3, Lakes in the Northeast Projected to  have  ANC Values
        < 0 and < 50 /xeq/L for Constant and Decreased
        Sulfur Deposition Cid
Number of Stream Reaches8
Time from
Present
(year)
0

20

50

Constant Deposition

ANC <0
Ob
0%
0
0%
129 (295)
10% (22%)

ANC <50
3b
0.2%
187 (310)
14% (23%)
203 (333)
15% (25%)
Decreased

ANC <0
Ob
0%
0
0%
159 (291)
12% (22%)
Deposition

ANC <50
3b
0.2%
187 (314)
14% (24%)
340 (359)
26% (27%)
' % is percentage of the target population of 1,323 stream
  reaches; () indicate 95 percent confidence estimates.
b Indicates estimate from National Surface Water Survey, Pilot
  Stream Survey sample for the same 30 streams; target
  population = 1,323 stream reaches.
0 Projections are based on 30 stream/watersheds successfully
  calibrated by MAGIC.
d See Figure 3 for definition of the deposition scenarios used.

 Table 4.  Southern Blue Ridge Province Stream Reaches Projected
         to have ANC  Values  < 0 and < 50ueq/L for Constant
         and Decreased Sulfur Deposition C|°


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o
CO
c
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0
CO
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o
'55
O
Q.
0)
O

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3



























*
X
X
X
X
xx Current
'\
\
\
"x^
\
\
\
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s








SBRP + 20%



(Base Case)





NE - 30%



- i .0
-1.4

-1.3

- 1 9
I .^

-1.1
O
+rf
-0 9

-0.8



-0.6

             10
                     20
                            30
                                    40
                                            50
                     Time (yr)
Figure 3.  Sulfur deposition scenarios for the Northeast (NE) and
         Southern Blue Ridge Province (SBRP) for Level H and
         Level III analyses.  Ratio of total sulfur deposition at
         time t (SJi to current total sulfur deposition (Sc).
                                                         -6-

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STATE
INFORMATION
The AERP status provides a forum for
states to exchange information and update
activities.  Highlighted state activities are
presented below.

California

As part of the five-year Atmospheric Acidity
Protection Program, the California Air
Resources Board recently funded three
major projects to investigate the effects of
acidic deposition on aquatic ecosystems of
the Sierra Nevada.   These projects include:

    •   Watershed Monitoring--^ contract
       was  awarded to the University of
       California, Santa Barbara, to monitor
       watershed and lake geochemistry
       year-round at seven high-elevation
       locations in the Sierra Nevada.  The
       focus of  this project will be the
       collection of long-term data on
       watershed characteristics and lake
       dynamics to determine the impacts
       of acidic  deposition.

    •  Alpine Wet Deposition Network--~tt\Q
       University of California, Santa
       Barbara,  was selected to install and
       operate a 10-station network for a
       period of 4  years to monitor the
       variability of wet deposition on the
       alpine zone in the Sierra Nevada on
       both  spatial and temporal scales.
       This  network will include snowpack
       sampling and rain event collections
       at elevations above 9,000 feet.
       These deposition data will be used
       to improve regional  lake acidification
       assessments.

    •  Aquatic Biota Stud/es-Contracls
       were awarded to investigators to
       carry out surveys  and dose-response
       studies of aquatic species in lakes
       and streams in the Sierra Nevada.
Both economically important species and
indicator species  will be studied during the
1990-91 field seasons.

Address inquiries on the above  information
to:

  Kathy Tonnessen
  Research Division
  Air Resources Board
  P.O. Box 2815
  Sacramento, California  95812
  (916) 324-1744

Florida

The Florida Department of Environmental
Regulation  has been conducting studies of
Florida's  sensitive lakes in order to
characterize their chemistry and biology,
evaluate historical changes in water
chemistry, and evaluate factors contributing
to their acid neutralizing capacity (ANC).
The Florida Soft Water Lakes Study project,
completed in the fall of 1989, evaluated the
fish status  and water chemistry of 12 acidic
soft water  lakes.  The Florida Sensitive
Lakes Reassessment Study project is
evaluating whether historical water
chemistry changes have occurred among
Florida lakes.  The Florida Seepage Lakes
Study is currently evaluating the factors that
regulate ANC,  including groundwater
contributions.

Address inquiries on above information to:

  Curtis E.  Watkins
  Florida Department of
    Environmental Regulation
  2600 Blair Stone Road
  Twin Towers Office Building
  Tallahassee, Florida 32399
  (904) 488-0782
                                            S-1

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                                                AERP status
                                          Announcement

   Due to AERP budget reductions and prioritization of research dollars within the program, publication of the
status will end with the next issue, which is scheduled for September 1990.

   In the final issue of the status, a reader-contribution section will replace the state activities section. The new
section will include articles on state activities but will  also present reader-contributed articles as well.  The reader-
contribution section is intended to be an open forum for exchange of information among acidic deposition
researchers.  All researchers are invited to contribute brief articles on monitoring and research activities in which
they are involved.  The deadline for submission is May 7 for publication in the September issue.


Please submit information or inquiries to:

   J.Y. Aoyama
   AERP State Information Coordinator
   1050 E. Flamingo, Suite 209
   Las Vegas, Nevada 89119
   (702) 734-3288
                                                   S-2

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                                                 AERP status
(referenced in footnote c/of both tables) is also
presented here.  The corrected values will  appear in
the published version of the final report for the DDRP
and do not affect the conclusions and discussion
presented in  the previous issue of the AERP status.
Address inquiries concerning DDRP to:

   M. Robbins Church
   DDRP Technical Director
   EPA/Environmental Research Laboratory-Corvallis
   200 S.W. 35th Street
   Corvallis, Oregon  97333
   (503) 757-4666, ext.  304
   FTS:  420-4666, ext. 304

Watershed Processes and Manipulations

The Watershed Manipulation Project, Watershed
Recovery Project, and Little Rock Lake Experimental
Acidification Project are watershed studies that were
undertaken to investigate the processes that
control the effects of acidic deposition on surface
waters. Progress in  each of these projects follow:
 Watershed Manipulation Project (WM^- Process-
oriented research in the WMP was designed to
examine the quantitative and qualitative responses of
watershed soils and surface waters to altered levels
of acidic deposition.  Manipulation studies have been
conducted at laboratory, plot, and catchment scales.
Using a paired catchment  approach, one catchment
was artificially acidified by applying ammonium
sulfate, with the other serving as  a control.  The first
manipulation occurred in November 1989.  At
catchments  located in southeastern Maine,  hypothesis
tests have been conducted through the cooperative
efforts of  a site team, six  scientific task teams, a
modeling team, and an EPA management team. The
laboratory and plot studies have yielded several
preliminary findings.  Results through June 1989 were
incorporated into a draft findings report that was
completed in December 1989.
Address inquiries concerning WMP to:

   Jeffrey J. Lee
   WMP Technical Director
   EPA/Environmental Research Laboratory-Corvallis
   200 S.W. 35th Street
   Corvallis, Oregon 97333
   (503) 757-4666, ext. 318
   FTS: 420-4666, ext. 318

Little Rock Lake Experimental Acidification Pro/ect--~The
first  year of the Little Rock Lake treatment basin
acidification to the lowest intended pH level of 4.6 is
progressing smoothly.  Approximately the same
amount of acid (400 liters) was added during the
summer of 1989 as was required to establish pH
levels of 5.6 and 5.1 in  1985 and 1987, respectively.
This  is approximately twice the amount of acid
required during  the second year at each pH level, or
approximately 4 and 2 times the background levels of
acidic deposition, respectively. A Mougeotia-
dominated algal complex appeared in greater
quantities this year than last (the second year at pH
5.1) but was still substantially less prevalent than
during the first year at pH 5.1. An  inconsistent
response of Mougeotia growth to low pH also has
been observed in  Max Lake, a  system located near
Little Rock Lake with a natural pH of 5.1. Blue-green
algae essentially have been eliminated in the
treatment basin but remain common in the reference
half  of the lake.  The treatment basin continues to be
more transparent on the average and more blue in
color than the reference basin. The reduction in
zooplankton species has accelerated at pH 4.6, with
only  5 of the approximately 20 or so  original, dominan
species remaining.  Many  indirect as well as direct
effects on zooplankton have been observed.

Reproduction  of largemouth bass and rock bass
ceased at pH 4.6.  Although adults nested and some
spawning occurred, no fry were produced. Adults of
both species appear physiologically and
morphologically normal, but population levels are
                                                    -7-

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                                                AERP status
dropping due to declining recruitment.  Complementary
laboratory studies have demonstrated that survival of
young-of-the-year largemouth bass is reduced under
water quality conditions (temperature, Ca2+, AI3+)
simulating the treatment basin of  Little Rock Lake at
pH 5.1 during the winter (113 days of exposure).
Yellow perch appear to be surviving and reproducing
as well or better in the treatment  basin as in the
reference basin.  Results from the summer of 1989 for
many chemical and  biological studies will be
presented in a subsequent issue of the status.
Several reports on the Little Rock  Lake project became
available during the  summer, including presentations
made in Geneva, Switzerland; Freiburg and Munich,
Germany; Toronto, Canada; Anchorage and Fairbanks,
Alaska; and Madison,  Wisconsin.

Address inquiries concerning the abovementioned
reports and presentations or the Little Rock Lake
Experimental Acidification Project to:

   John Eaton
   Little Rock Lake  Experimental Acidification Project
     Technical Director
   EPA/Environmental Research Laboratory-Duluth
   6201 Congdon Boulevard
   Duluth, Minnesota 55804
   (218) 720-5557
   FTS:  780-5557

Episodic Response Project  (ERP)

Episodic acidification is the process by which lakes
and  streams experience short-term decreases of ANC.
An episode is an occurrence of a  short-term decrease
of ANC, usually during hydrological events (periods of
increased streamflow  due to rainstorms or snowmelt)
and  over time scales of hours to  weeks.  Typically,
changes in other water quality parameters, such as
pH, base cations, or species of dissolved aluminum,
accompany episodes.   Changes in calcium and
aluminum have the potential to impact aquatic biota.

In the past, most approaches to quantifying episodes
have been only partially successful, primarily because
of the unpredictable nature of rainstorms and
snowmelt.  As a result, the ERP is being conducted to
answer key questions about episodic acidification.
The  ERP goals are to  (1) quantify the occurrence of
episodes in four or five streams in each of three
areas, the Northern Appalachian Plateau in
Pennsylvania and the Adirondack and Catskill
Mountains in New York state, (2) describe biological
responses to episodes, and (3) develop and  evaluate
regionally applicable models of episodic acidification.

Eastern Episodes-One year of field research has beer
completed, the results of which are being analyzed
and will be included in the upcoming NAPAP  State-of-
Science reports and Integrated Assessment.
Cooperating research groups at the Adirondack Lakes
Survey Corporation, Pennsylvania State University, and
the United States Geological Survey conducted
intensive biological and hydrochemical research during
the fall of I989.  Biological experiments include wild
fish transplants  and population-level assessments,
field bioassays,  and radiotelemetry work.
Hydrochemical data are being collected with a
combination of automated and manual sampling
approaches.

Address inquiries concerning ERP to:

     Parker J. Wigington, Jr.
     ERP Technical Director
     EPA/Environmental Research Laboratory-Corvallis
     200 S.W. 35th Street
     Corvallis, Oregon 97333
     (503) 757-4666, ext. 354
     FTS: 420-4666, ext. 354

Regional Episodic and Acidic Manipulations Project
(f?EAMJ~AHer manipulation of whole catchments by
enhancing acidic deposition, REAM studied and
provided data of its effects on surface water quality.
At the  United States Department of Agriculture  (USDA)
Forest Service at Fernow Experimental Forest near
Parsons, West Virginia, scientists  monitored the
responses of streams to acidification on both chronic
and episodic time scales.

Using a paired catchment approach, one catchment
was artificially acidified by applying ammonium
sulfate, with the other  serving as a control.
Manipulations began in January 1989.  Subsequent
applications followed in July and October. Application
rates of sulfate were approximately three times the
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                                                AERP status
seasonal ambient rate. Annual ambient sulfate
deposition at Fernow was about 790 eq/ha. Monthly
ambient sulfate deposition was  about 25 eq/ha in
January, 150 eq/ha in July, and 40  eq/ha in October.

Observations in both streams at the site have shown
episodic depressions in pH and  increases in sulfate
concentrations associated with storms. For the most
part, these changes were not associated with the
manipulations.  The only changes in stream chemistry
caused by the applications of ammonium sulfate were
transient increases in ammonium and sulfate
concentrations during the first storm following each
application.  These changes were caused by runoff of
ammonium sulfate, and were  an artifact of the
application methods. Oxygen-18 data for stream
water, soil water, and precipitation was used to
examine hydrologic routing in  the catchments.
Biological studies have been funded and initiated by
the USDA Forest Service at the site.

Address inquiries concerning REAM to:

   Jeffrey J. Lee
   REAM Technical Director
   EPA/Environmental Research  Laboratory-Corvallis
   200 S.W. 35th Street
   Corvallis, Oregon 97333
   (503) 757-4666, ext. 318
   FTS: 420-4666. ext. 318

SYNTHESIS AND INTEGRATION
ACTIVITIES	

State-of-Science/Technology (SOS/7} Reports-The
seven Aquatic Effects Task Group  SOS/T reports will
be presented in February at an international meeting
at Hilton Head Island, South Carolina.  Summaries for
each of the 28 NAPAP SOS/T reports, including the
aquatic effects reports, will be distributed to all
participants at the meeting. Each  participant will
receive a copy of one SOS/T report of their choice.
Additional reports can be purchased.  To receive a
copy of the Summaries document or to purchase
individual SOS/T reports, please  contact:
   Patricia Irving
   Office of  the Director
   National Acid Precipitation Assessment Program
   722 Jackson Place, N.W.
   Washington, D.C. 20503
   (202) 395-5771

Technical Information Project~Jbe Technical
Information Project disseminates information on AERP
activities to state agencies, organizations, and
technical audiences. Distributed information includes
the following items:

   •  Major  Report with Companion Documents -
      These document  sets consist of a compilation
      of the manuals and  reports used during or
      prepared as a result of a particular AERP
      project.  Companion documents to each major
      data report include field operations and quality
      assurance reports, quality assurance plans, and
      analytical methods manuals. Document sets
      for the Eastern Lake Survey - Phase I, Western
      Lake Survey, National Stream Survey, and DDRP
      are available through the mail order form in this
      status.

   •  Data  Bases - Each data base consists of two
      components:  (1) a computer diskette or  tape
      containing the validated data base for a
      particular AERP project, and (2) a user's guide
      with instructions  on  how to use the disk  or
      tape.  Information about how the quality of the
      data was assessed  is included. Data bases fo
      the Eastern Lake Survey - Phase I and Western
      Lake Survey are available through the mail orde
      form in this status.

   •  Handbooks - Handbooks are guidance
      documents that contain procedures for field  am
      laboratory operations for surface water and  soi
      chemistry sampling.  They are beneficial to
      those  organizations  involved in designing and
      implementing monitoring activities related to
      acidic deposition. The Handbook of Methods
      for Acid Deposition Studies, Laboratory Ana/ysi
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                                               AERP status
   for Surface Water Chemistry is available through
   the order form in this issue.

NOTE:   Due to a printing error, a  draft version of
        the Handbook of Methods for Acid
        Deposition Studies, Field Operations for
        Surface Water Chemistry, EPA/600/4-89/020,
        was published and distributed. If you
        have received a copy of this document,
        please destroy it. The correct copy will be
        sent to you as soon as it is available.

Address inquiries concerning the AERP Technical
Information Project to:

   Daniel T.  Heggem
   AERP Technical  Information Project
    Technical Director
   EPA/Environmental Monitoring Systems Laboratory
   P.O. Box 93478
   Las Vegas, Nevada 89103
   (702) 798-2358
   (FTS):  545-2358

AERP ANNOUNCEMENTS	

The Eastern  Lake Survey-Phase II (ELS-II)
zooplankton  data are now available on magnetic
computer tapes and in two reports  by A.J. Tessier and
R.J. Horowitz.

The first report, entitled Analysis and Interpretation of
Zooplankton Samples Collected During Phase II of the
National Lake Survey (Academy of National Sciences
of Philadelphia (ANSP) Report #88-18), contains data
and analyses of samples collected from 146 lakes in
the Northeastern United States:  the abundance of
each  species and each size class of  zooplankton,
lake variance in zooplankton assemblages, and the
composition of zooplankton assemblages in relation
to physical/chemical features of the lakes.

The second report, entitled Zooplankton Community
Patterns and Relationships to Environmental
Parameters in High Elevation Lakes in Maine (ANSP
Report #88-25), includes data on zooplankton samples
taken from 83 lakes in Maine, and  the relationships
between zooplankton assemblages and the
physical/chemical parameters of the lakes.
Address inquiries about acquisition of the ELS-II
zooplankton data and reports to:

   Donald Charles
   EPA/Environmental Research Laboratory
   200 S.W. 35th Street
   Corvallis, Oregon 97333
   (503) 757-4666, ext. 428
   FTS: 420-4666, ext. 428

COMPLETED AERP ACTIVITIES

Listed below is an AERP document that is now
available through the order form in this status.

    The Direct/Delayed Response Project: Quality
   Assurance Plan for Preparation and Analysis of
   Soils from the Mid-Appalachian Region of the
   United States addresses the design and
   implementation of a quality assurance program
   and the verification of the analytical data base  for
   the Mid-Appalachian Soil Survey.  It is addressed
   primarily to users of the data base who  will be
   analyzing the data and making various
   assessments and conclusions  relating to the
   effects of acidic deposition on  the soils of the Mid-
   Appalachian Region, the third and final region
   characterized during the project.
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AERP status
                           DATE DUE
 If you would  like to  receive any of  the following
 AERP  products,  please  check  the  appropriate
 box(es) and fill in your name and address below.
MAJOR REPORT/COMPANION DOCUMENTS
Eastern Lake Survey - Phase I
 Major Report - Characteristics of Lakes in the Eastern United
   States
    Volumes Mil	 600/4-86/007  —
    Volume I	600/4-86/0073 —
    Volume II  	600/4-86/007b —
    Volume III	600/4-86/007C	
 Quality Assurance Plan	 600/4-86/008  —
 Analytical Methods Manusl	 600/4-86/009  —
 Field Operations Report 	 600/4-86/010  —
 Quality Assurance Report	600/4-86/011  —
Western Lake Survey  - Phase I
 Major Report - Characteristics of Lakes in the Western United
   States
    Volumes I-II (Volume I  out of print) . . . 600/3-86/054  —
    Volume II  	600/3-86/054b —
 Quality Assurance Plan	 600/8-87/026  —
 Analytical Methods Manual	 600/8-87/038  —
 Field Operations Report 	 600/8-87/018  —
 Quality Assurance Report	 600/4-87/037  —
National Stream Survey - Phase I
 Major Report - Characteristics of  Streams in the Mid-Atlantic
   and Southeastern United States
    Volumes I-II 	 600/3-88/021  —
    Volume I	 600/3-88/0213 —
    Volume II  	 600/3-88/021b —
 Pilot Survey Major Report  	 600/4-86/026  —
 Pilot Survey Field Operations Report  .... 600/8-87/019  —
 Quality Assurance Plan	 600/4-86/044  —
 Field Operations Report 	 600/4-88/023  —
 Processing Lsboratory Report	 600/4-88/025  —
 Quality Assurance Report	 600/4-88/018  —
Direct/Delayed  Response Project
 Quality Assurance Report
   Southern Blue Ridge Province	 600/8-88/100  —
                          ••  ~> protection
      U"J    ''    *.'".'!'    - .1:0
           Analytical Methods Manusl	  600/8-87/020  —
         * Quality Assurance Plan
             Mid-Appalachian Region	  600/4-89/031  —
           Field Operations Report
             Southern Blue Ridge Province
              Volume I-II  	  600/4-87/041  —
              Volume I 	600/4-87/041a —
              Volume II	600/4-87/041b —
           Quality Assurance Plan	  600/8-87/021  —
         DATA  BASES
           Western Lake Survey Data Base
             (special order form will be sent)
           Eastern Lake Survey - Phase I Data
             Base (special order form will be sent)
         HANDBOOKS
.  600/4-87/027

.  600/4-88/032
           Handbook of Methods for Acid Deposition
             Studies,  Laboratory Analysis for Surface
             Water Chemistry 	  600/4-87/026
           Handbook of Methods for Acid Deposition
             Studies,  Field Operations for Surface
             Water Chemistry 	  600/4-89/020
         PROJECT DESCRIPTORS
           Research Activity Descriptors, FY 1988  .
           Research Activity Descriptors, FY 1989  .
         ABSTRACTS
           Biennial Publications and Presentations
             1985-86  	
  600/9-88/006
  600/9-89/059
  600/9-88/018 —
              Name
              Address
              City/State/Zip,
              Return to:
              CERI, AERP Publications
              U.S. Environmental Protection Agency
              26 W. Martin Luther King Drive
              Cincinnati. Ohio 45268
         *Publication listed for the first time.
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