United States          Office of          SS'S±2*
           Environmental Protection      Solid Waste and       EPA/540/R/94/083
           Agency            Emergency Response     PB95-963525
                                       December1994
           Superfund

vxEPA     LABORATORY DATA VALIDATION
            FUNCTIONAL GUIDELINES FOR
            EVALUATING INORGANICS
            ANALYSES
                 REPRODUCED BY
                 U.S. DEPARTMENT OF COMMERCE
                    NATIONAL TECHNICAL
                    INFORMATION SERVICE
                    SPRINGFIELD, VA 22161

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                                                           9240.1-26
                                                           PB95-963525
                                                           EPA540/R-94/083
                  LABORATORY DATA VALIDATION

FUNCTIONAL GUIDELINES'FOR EVALUATING INORGANICS ANALYSES
                                                          t


                          Prepared for the
             HAZARDOUS SITE EVALUATION DIVISION
            U.S. ENVIRONMENTAL PROTECTION AGENCY
                            Compiled by
                           Ruth Bleyler
                     Sample Management Office
                          Viar St Company
                            Prepared by
                The USEPA Data Review Work Group
           Jeanne Hankins - EPA Region III - Chairperson
            Frank Messina, Laura Scalise - EPA Region II
                   Gary Bennett - EPA Region IV
                     Ida Levin - EPA Region V
                Mahmoud EI-Feky -  EPA Region VI
                  Larry Marchin - EPA Region VII
                          July 1, 1988
               U.S. Environmental Protection Agency
               Region 5, Library (PL-12J)
               77 West Jackson Boulevard, 12th Floor
               Chicago, IL  60604-3590                              //88

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                                            TABLE OF CONTENTS
                                                                                             Page

              INTRODUCTION	1

              PRELIMINARY REVIEW	2

              INORGANICS PROCEDURE	2

              I.      Holding Times	3

              II.     Calibration	4

             HI.     Blanks	6

             IV.     ICP Interference Check Sample (ICS)	7

             V.     Laboratory Control Sample (LCS)	9
^
v            VI.     Duplicate Sample Analysis	10

 |           VII.   Matrix Spike Sample Analysis	II

 :            VIII.   Furnace Atomic Absorption QC	12
 !
             IX.    ICP Serial Dilution	13

 ,           X.     Sample Result Verification	14

             XI.    Field Duplicates	 14

            XII.    Overall Assessment of Data for a Case	15

            GLOSSARY A: Data Qualifier Definitions	16

            GLOSSARY B: Additional Terms	17
                                                                                          7/88

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                                      INTRODUCTION

         This document  is  designed to  offer  guidance  in  laboratory data  evaluation  and
  validation.   In some aspects,  it is  equivalent to a Standard  Operating Procedure (SOP).  In
  other, more subjective areas,  only  general  guidance is  offered due to the complexities  and
  uniqueness  of data relative to specific  samples.  These Guidelines have  been updated to
  include all  requirements in the 7/87 Statement of Work (SOW) for  Inorganics, Amendment 1
  and December 1987 Revisions.

         Those areas  where specific SOPs  are possible are primarily  areas in which definitive
  performance  requirements  are  established.     These  requirements  are  concerned  with
  specifications that are not  sample dependent;  they specify  performance  requirements  on
  matters that should be fully under a laboratory's control.   These specific areas include blanks,
  calibration  standards,  calibration verification standards, laboratory control standards,  and
  interference check standards.   In particular, mistakes such as calculation and transcription
  errors must  be rectified by resubmission of corrected data sheets.

        This  document  is intended  for  technical review.   Some areas of overlap between
  technical  review and  Contract Compliance Screening (CCS)  exist;  however, determining
  contract compliance is not intended to be a  goal of these guidelines.  It is assumed that the
  CCS is available and can be utilized  to assist  in the data review procedure.

        At times,  there  may  be an urgent need  to use data which do not meet all contract
  requirements and  technical criteria.   Use of these  data does  noj  constitute  either a  new
  requirement standard or full  acceptance of the data.   Any decision to utilize data for which
  performance criteria have not  been met is strictly  to  facilitate the  progress of  projects
 requiring the availability of the data.  A contract laboratory submitting data which are out of
 specification may be required to rerun or resubmit data even if the previously submitted data
 have  been  utilized  due  to  urgent program  needs; data  which  do  not  meet specified
 requirements are  never  fully acceptable.  The only exception to this requirement is in the
 area of requirements for individual sample analysis; if the nature of the sample itself limits
 the attainment of specifications,  appropriate allowances  must  be  made.   The  overriding
 concern of the Agency is to obtain data which are technically valid and legally defensible.

        All  data  reviews  must have,  as a cover  sheet,  the  Inorganic Regional  Data
 Assessment (IRDA) form.  (A copy is attached at the  end of this document)  If mandatory
 actions are required, they should be specifically noted on this form. In addition, this form is
 to be used to summarize overall deficiencies requiring attention, as well as general laboratory
 performance  and  any discernible trends in  the  quality of  the data. (This form is  not a
 replacement for the data review.)  Sufficient supplementary documentation  must accompany
 the  form to  clearly  identify the  problems  associated with a Case.  The form  and  any
 attachments  must  be  submitted to  the  Contract Laboratory  Program Quality Assurance
 Coordinator (CLP QAC), the Regional Deputy Project Officer (DPO),  and the Environmental
 Monitoring Systems Laboratory in Las Vegas (EMSL/LV).

       It is the responsibility of the data  reviewer to  notify the Regional DPO concerning
problems and shortcomings with  regard to laboratory data.  If there is an urgent requirement,
the DPO may be contacted by telephone  to expedite corrective action.  It  is recommended
that all items for DPO action be presented  at one  time.  In any case, the Inorganic  Regional
Data Assessment form must be completed and submitted.
                                                                                   7/88

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                                 PRELIMINARY REVIEW


       In order to use this document effectively, the reviewer should have a general overview
 of the Case at hand.  The exact number of samples, their assigned numbers, their matrix, and
 the number of laboratories involved in their analysis  are essential information.   Background
 information on the site is helpful but often this information  is very difficult to locate.  The
 site project officer is the best source for answers or further direction.

       CCS is a source of a large quantity of summarized information.  It can be  used  to alert
 the reviewer of  problems in  the Case or what  may be  sample-specific  problems.   This
 information may be utilized in data validation.  If CCS is unavailable, those criteria affecting
 data validity must be addressed by the data reviewer.

       Cases routinely have unique samples which  require  special attention  by the reviewer.
 Field  blanks, field  duplicates, and  performance audit samples need to be  identified.  The
 sampling records should provide:

            1.    Project Officer for site

            2.    Complete list of samples  with  notations on

                 a)    sample matrix

                 b)    blanks*

                 c)    field duplicates*

                 d)    field spikes*

                 e)    QC audit sample*

                 0     shipping dates

                 g)    labs involved

                 * If applicable

     The  chain-of-custody  record  includes sample  descriptions  and  date of sampling.
Although sampling date is not addressed by contract requirements,  the  reviewer  must take
into account lag time between sampling and shipping while assessing sample holding times.

                              INORGANICS PROCEDURE
     The requirements to  be  checked in validation are listed below.   ("CCS" indicates  that
the contractual requirements for these items will also be checked by CCS;  CCS requirements
are not always the same as the data review criteria.)

     I.     Holding Times (CCS - Lab holding times only)
                                                                                   7/88

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       II.    Calibration

             o     Initial (CCS)

             o     Initial and Continuing Calibration Verification (CCS)

       III.    Blanks (CCS)

       IV.    ICP Interference Check Sample (CCS)

       V.    Laboratory Control Sample (CCS)

       VI.   Duplicate Sample (CCS)

       VII.  Matrix Spike Sample (CCS)

       VIII.  Furnace Atomic Absorption QC (CCS)

       IX.   ICP Serial Dilution (CCS)

       X.    Sample Result Verification (CCS - 10%)

       XL   Field Duplicates

      XII.   Overall Assessment of Data for a Case


                                  I.  HOLDING TIMES

A.     Objective

       The objective is to  ascertain the validity of results based on the holding time of the
       sample from time of collection to time of analysis.

       Note: The holding  time is based on the  date of collection, rather than verified time
       of sample receipt, and date of digestion/distillation. It is a technical evaluation rather
       than a contractual requirement

B.     Criteria

       Technical requirements for sample holding times have only been established for water
       matrices.  The following holding time and preservation requirements were established
       under 40  CFR 136  (Clean Water Act) and are found  in Volume 49, Number 209 of
       the Federal Register, page 43260, issued on October 26, 1984.

           METALS:        6 months;  preserved to pH < 2

           MERCURY:      28 days; preserved to pH < 2

           CYANIDE:       14 days; preserved to pH > 12
                                                                                 7/88

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 C.     Evaluation Procedure

        Actual  holding times are established  by comparing the sampling date on the EPA
        Sample  Traffic Report with  the  dates  of analysis found in the laboratory raw data
        (digestion logs and  instrument  run logs).   Examine the digestion  and/or distillation
        logs to determine if samples were preserved at the proper pH.

        Analyte Holding Time (Days) =  Analysis Date -  Sampling Date

 D.     Action

        1.      If 40 CFR 136 criteria for holding times and preservation are not met,  qualify
               all results  > Instrument Detection  Limit (IDL) as  estimated  (J)  and  results
               < IDL as estimated (UJ).

        2.      If holding times are exceeded,  the reviewer must use professional judgment to
              determine the reliability of the data and the effects of additional storage on
              the  sample results. The  expected bias would be  low and  the  reviewer  may
              determine that results < IDL are unusable (R).

        3.     Due to limited information concerning holding times for soil samples, it is left
              to the  discretion of the  data reviewer whether to apply water  holding  time
              criteria  to soil  samples.   If the  data are qualified when water  holding  time
              criteria  are  applied  to soil samples,  it  must be  clearly  documented  in the
              review.


                                   II.  CALIBRATION

A.     Objective

       Compliance requirements  for satisfactory instrument calibration are  established to
       ensure that  the instrument is  capable  of  producing   acceptable quantitative  data.
       Initial calibration   demonstrates  that  the  instrument  is   capable of  acceptable
       performance at the  beginning  of  the  analysis  run,  and continuing calibration
       verification  documents that the initial calibration is still valid.

B.     Criteria

       1.      Initial Calibration

              Instruments must be calibrated daily and each time the instrument is set up.

              a.      ICP Analysis

                     A blank and at least one  standard must  be  used  in  establishing  the
                     analytical  curve.

              b.      Atomic Absorption Analysis (AA)

                     1)  A  blank and at least three standards, one of which must  be at the
                         Contract Required  Detection  Limit   (CRDL),  must  be  used  in
                         establishing  the analytical curve.
                                                                                    7/88

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                       2)  The correlation coefficient must be >0.995.

                           Note:  The correlation coefficient of 0.995 is  a  technical criterion
                           and  not contractual.

               c.      Mercury Analysis

                       I)  A  blank and  at least  four standards must be  used in establishing
                           the analytical  curve.
                       2)  The  correlation coefficient must be >0.995.

               d.      Cyanide Analysis

                       I)   A blank  and at  least three standards must be  used in establishing
                           the analytical curve.

                       2)   A midrange standard must be distilled.

                       3)   A  correlation  coefficient  >0.995  is required  for  photometric
                           determination.

        2.      Initial and Continuing Calibration Verification (ICV and CCV)

               a.      Analysis results must fall  within the control limits  of 90 -110 percent
                      Recovery (%R) of the  true  value  for all analytes except mercury and
                      cyanide.

               b.      Analysis results for mercury must fall within  the control limits of 80-
                      I20%R.

              c.      Analysis  results for cyanide  must fall within the control limits of 85-
                      HS%R.

C.     Evaluation Procedure

       1.     Verify that the instrument was calibrated  daily and each time the instrument
              was set up using the correct number of standards and blank.

       2.     Verify that the correlation coefficient is >0.995

       3.     Check the  distillation  log  and  verify that the midrange  CN  standard was
              distilled.

       4.     Recalculate one or more of the ICV and CCV %R per type of  analysis  (ICP,
              GFAA,  etc.)  using  the following equation  and verify that the recalculated
              value agrees with  the laboratory reported values on Form HA.  Due  to possible
              rounding discrepancies, allow results  to fall within 1% of the  contract windows
              (e.g.,89-111%).

                            %R .  Found x  100
                                   True

              Where,

              Found =  concentration (in  ug/L) of each analyte measured in the analysis of
                       the ICV or CCV solution


                                              5                                      7/88

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                True =   concentration (in ug/L) of each analyte in the ICV or CCV source

 D.      Action

         1.      If the minimum number of standards as defined in section B were not used for
                initial calibration, or if the instrument was not calibrated daily and each time
                the instrument was set up, qualify the data as unusable (R).

         2.      If the correlation  coefficient is <0.995, qualify results > IDL as estimated (J),
                and  results < IDL  as estimated  (UJ).

         Note:   For critical  samples, further  evaluation of  the calibration  curve may  be
         warranted to determine if qualification is necessary.
        3.     If the midrange CN standard was not distilled, qualify all associated results as
               estimated (J).

        4.     If the ICV or CCV %R falls outside the acceptance windows, use professional
               judgment to qualify all associated data.   If possible,  indicate  the  bias in  the
               review.  The following guidelines are recommended:

               a   If the  ICV or CCV %R falls outside the acceptance windows but within
                   the ranges of 75-89% or 111-125% (CN. 70-84% or  116-130%; Hg. 65-
                   79% or 121-135%). qualify results > IDL as estimated (J).
               b.  If the ICV or CCV %R is  within the range of 111-125% (CN. 116-130%;
                   Hg, 121-135%). results < IDL are acceptable.
               c.   If the  ICV or CCV %R is 75-89%  (CN, 70-84%; Hg, 65-79%), qualify
                   results  < IDL as estimated (UJ).
               d.   If the  ICV  or CCV  %R  is <75%,  (CN, <70%; Hg.  <65%), qualify all
                   positive results as unusable  (R).

               e.   If the ICV or CCV %R is >125%, (CN >130%; Hg >135%).  qualify results
                   > IDL as unusable (R); results < IDL are acceptable.


                                      III. BLANKS

A.     Objective

       The assessment of blank analysis results is to determine the existence and magnitude
       of contamination problems. The criteria for evaluation of blanks applies to  any blank
       associated with the samples.  If problems with anv blank exist, all data associated with
       the Case must be carefully evaluated to determine whether or not there  is an inherent
       variability in  the data for the Case, or if the  problem is  an isolated occurrence  not
       affecting other data.

B.     Criteria

       Nc contaminants should be in the blank(s).
                                                                                    7/88

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  C.      Evaluation Procedures

         Review the results reported on the  Blank Summary (Form III) as well as the raw data
         (ICP printouts, strip charts, printer  tapes, bench sheets, etc.) for all blanks and verify
         that the results were accurately reported.

  D.      Action

         Action in the case of unsuitable blank results depends on  the circumstances and origin
         of the blank. Sample results  > IDL but  <5  times  the amount in any blank should be
         qualified as (U).

         Any  blank  with  a negative result whose absolute value  is > IDL must be carefully
         evaluated to determine its effect on  the sample data.

         Note:  The blank analyses may  not involve the same weights, volumes,  or  dilution
         factors as the associated samples.  In particular, soil sample results reported on Form I
         will  not be on the same basis (units, dilution) as the calibration blank data reported on
         Form HI.  The reviewer may find it easier toovork f.^m  the raw data when applying
         5X criteria to soil sample data/calibration blank data.

        In instances  where  more  than  one  blank  is  associated  with  a  given  sample,
        qualification should be based upon a comparison  with the  associated blank  having  the
        highest concentration of a contaminant   The results  must  not be  corrected  by
        subtracting any blank value.


                     IV.  ICP INTERFERENCE CHECK SAMPLE CICS)

 A.     Objective

        The ICP Interference Check Sample verifies the contract laboratory's interelement and
        background correction factors.

 B.      Criteria

        1.     An ICS must be run at  the  beginning  and end  of each sample analysis run (or
              a minimum of twice per 8 hour working shift, whichever is more frequent).

       2.     Results for the ICS solution AB analysis must fall within the control limits of
              ± 20% of the true value.

C.     Evaluation Procedure

        1.     Recalculate from  the raw data (ICP printout) one or more of the recoveries
              using the following equation (%R) and  verify that the recalculated value agrees
              with the laboratory reported values on Form IV.

                            ICS %R =     Found Solution AB     .««
                                         True Solution AB
                                                                                   7/88

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               Where,

               Found Solution AB  =  concentration (in ug/L) of each analyte measured  in
                                     the analysis of solution AB

              True Solution AB    =  concentration (in ug/L) of each anaiyte in solution AB

       2.     Check ICS raw data for results with an absolute value > IDL for those analytes
              which  are not present in the ICS solution.
D.     Action
       I.      For samples with concentrations of Al, Ca, Fe, and Mg which are comparable
              to or greater than their respective levels in  the Interference Check Sample:

              a.      If the ICS recovery for an element is >120% and the sample results are
                     < IDL, this data is acceptable for use.

              b.      If the ICS recovery for an eleofent is >I20% and the  sample results are
                     > IDL. qualify the affected data as estimated (J).

              c.      If the ICS recovery for an element  falls  between 50  and 79% and  the
                     sample results are > IDL, qualify the affected data as estimated (J).

                IDL are observed for elements which are not present in the EPA
              provided  ICS solution, the possibility of false positives exists.  An evaluation
              of the associated sample data for  the affected elements should be made.  For
              samples with comparable or  higher levels  of  interferents and  with  analyte
              concentrations that approximate those levels  found in die ICS (false positives),
              qualify sample results > IDL as estimated (J).

      3.      If negative  results are observed for elements that are not present in the EPA
              ICS  solutions,  and their  absolute  value is   >  IDL,  the possibility of  false
              negatives  in the samples  may exist.   If the absolute value  of  the negative
              results is  >  IDL, an evaluation of the associated sample data should be made.
              For samples with comparable or higher levels of  interferents, qualify results
              for the affected  analytes < IDL as estimated (UJ).

      4.      In general, the sample data can be accepted  if  the concentrations of Al, Ca,
              Fe and  Mg in the sample are found to be less than or equal  to  their  respective
             concentrations in the ICS.   If these  elements  are present at concentrations
              greater  than the level in the  ICS, or other elements are present in the sample
             at >10 mg/L,  the   reviewer should  investigate  the  possibility  of  other
              interference effects by using  Table 2  given  on page  D-22 of  the 7/87  SOW.
              These  analyte concentration   equivalents presented  in  the  Table  should be

                                             8                                      7/8S

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                considered  only as estimated values, since the exact value of  any analytical
                system is instrument specific.  Therefore, estimate the concentration produced
                by an interfering element.  If the estimate is >2X CRDL and also greater than
                10%  of  the  reported  concentration of  the  affected  element, qualify  the
                affected results as estimated (J).


                       V.  LABORATORY CONTROL SAMPLE (LCS1

 A.     Objective

        The laboratory control sample serves as a monitor of the overall performance of all
        steps in the analysis, including the  sample preparation.

 B.     Criteria

        1.     All aqueous LCS results must fall within  the control  limits  of 80-I20%R,
               except Sb and Ag which have no control limits.

        2.     All solid  LCS  results  must fall within  the control  limits established by  the
               EPA.  This information is available from EMSL/LV.

 C.     Evaluation Procedure

        1.     Review Form VII and verify that results fall within the control limits.

        2.      Check  the raw data (ICP printout, strip charts, bench sheets) to verify  the
               reported recoveries on Form VII.  Recalculate one or more of  the recoveries
               (%R) using the following equation:

                            LCS %R -    LCS Fpynd   x I(X)
                                          LCS True

              Where,

              LCS Found   -    concentration (in ug/L for aqueous; mg/kg for solid) of each
                               analyte measured in the analysis of LCS solution

              LCS True    -    concentration (in ug/L for aqueous; mg/kg for solid)  of each
                               analyte in the LCS source

D.     Action

       1.     Aqueous LCS

             a.     If the  LCS recovery for any analyte falls within the range  of 50 - 79%
                    or >120%.  qualify results > IDL as estimated (J).

             b.     If results are < IDL  and the  LCS  recovery is  greater than  120%, the
                    data are acceptable.

             c.     If results are < IDL and  the LCS recovery  falls within the range  of 50-
                    79%, qualify the data for the affected analytes as estimated  (UJ).
                                                                                   7/88

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               d.      If LCS recovery results are <50%, qualify the data for these samples as
                      unusable (R).

        2.      Solid LCS

               a.      If the solid LCS recovery for any analyte falls outside the  EPA control
                      limits, qualify all sample results > IDL as estimated (J).

               b.      If the LCS results  are  higher than the control  limits and  the sample
                      results are < IDL, the data are acceptable.

               c.      If the LCS results are lower than the control limits,  qualify all sample
                      results < IDL  as estimated (UJ).


                           VI. DUPLICATE SAMPLE ANALYSIS

 A.    Objective

       Duplicate analyses are indicators of laboratory precision based on each sample  matrix.

 B.    Criteria

       1.     Samples identified as  field blanks cannot be used for duplicate sample analysis.

       2.     A control limit  of ± 20% (35%  for soil) for the Relative Percent Difference
              (RPD) shall be used for sample values >5X CRDL.

       3.     A control limit  of tCRDL (±2X CRDL for soil) shall  be  used for sample
              values <5X CRDL, including the case when only one of the  duplicate sample
              values is <5X CRDL.

C.     Evaluation Procedure

       1.     Review Form VI and  verify that results fall within the control limits.

       2.     Check the raw data  and recalculate one or  more RPD using the following
              equation to verify that results  have been correctly reported on  Form  VI.
                            RPD-

              Where,

              S   -  First Sample Value (original)
              D   «=  Second Sample Value (duplicate)

       3.      Verify that the field blank was not used for duplicate analysis.

D.     Action

       I.      If duplicate  analysts results for a particular analyte fall outside the appropriate
              control windows,  qualify the results  for that ai.ulyte  in all associated samples
              of the same  matrix as estimated (J).

                                              10                                     7/88

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        2.      If the field blank was 'used for duplicate analysis, all other QC data must be
                carefully  checked and  professional  judgment  exercised  when  evaluating  the
                data.

                Note:  This information must be included on the IRDA form.


                         VII.  MATRIX SPIKE SAMPLE ANALYSIS

 A.     Objective

        The matrix spike sample analysis provides information about the effect, of each sample
        matrix on the digestion and  measurement methodology.

 B.     Criteria

        1.      Samples identified as field blanks cannot be used for spiked sample analysis.

        2.      Spike recovery (%R) must  be within the limits of 75-125%.  However, spike
               recovery limits  do not  apply  when  sample concentration exceeds  the  spike
               concentration by a factor of 4 or more.

 C.     Evaluation Procedure

        1.      Review Form V and  verify that results fall within the specified limits.

        2.      Check  raw data and  recalculate  one or more %R using the following equation
               to verify that results were correctly reported on Form V.

                            %R =  (SSR-SR)   x 100
                                      SA

               Where,

              SSR  =  Spiked Sample Result
              SR   -  Sample Result
              SA   *  Spike Added

       3.      Verify that the field blank was not used for spike analysis.

D.     Action

       1.      If the spike recovery  is >I25% and the reported sample results are < IDL, the
              data is acceptable for  use.

       2.      If the spike recovery is >125%  or <75%  and the sample results are  > IOL,
              qualify  the data for these samples as estimated (J).

       3.      If the spike recovery  falls within the range of 30-74% and the sample results
              are <  IDL, qualify the data for these samples as estimated (UJ).

       4.-     If spike recovery results  fall  <30% and the sample results are < IDL, qualify
              the data for these samples as unusable (R).
                                                                                    7/88

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        5.     If the field blank  was used for matrix  spike analysts, all other QC data must
               be carefully checked and professional judgment exercised when evaluating the
               data.

               Note:  This information must be included on  the IRDA form.

        Note:  If the  matrix spike recovery does not meet criteria  (except  in  Ag), a post
        digestion spike is  required for all methods except furnace, but this data is not used to
        qualify  sample  results.  However,  this information must be included in the IRDA
        report.
                       VIII.  FURNACE ATOMIC ABSORPTION PC

 A.     Objective

        Duplicate injections and furnace  post  digestion spikes establish the precision and
        accuracy of the individual analytical determinations.

 B.      Criteria

        I.      For sample  concentrations >  CRDL, duplicate  injections must agree within
               ±20%  Relative Standard Deviation (RSD), (or Coefficient of  Variation (CV)),
               otherwise the sample must be rerun once (at least two additional injections).

        2.      Spike  recovery must be >85% and <115%.

        3.      The Furnace  Atomic Absorption Scheme must be followed as  described in the
               7/87 SOW, p. E-15.

C.      Evaluation Procedure

        1.     Check raw data to verify that duplicate injections agree within ±20% RSD (or
              CV) for sample concentrations > CRDL.

       2.     Review Furnace  AA raw data to verify that the Furnace Atomic Absorption
              Scheme has been followed.

D.     Action

        1.     If duplicate injections are outside the ±20% RSD (or CV) limits and the sample
              has not been rerun once as required, qualify the data as estimated (J).

       2.     If the rerun sample results do not agree within ±20%  RSD (or CV), qualify the
              data as estimated (J).

       3.     If the post digestion spike recovery is <40%, qualiiy results > IDL as estimated
              (J).

       4.     If the  post digestion spike recovery  is >IO%,  but <40%, qualify results < IDL
              as estimated (UJ).

       5.     If the po*t digestion spike recovery is <10%, qualify results < IDL as  unusable
              (R).

                                              12                                    7/88

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         6.     If sample absorbance h <50% of the post digestion spike absorbance then:

                a.  If the  furnace pose  digestion  spike recovery  is  not within  85-115%,
                    qualify the sample results > 1DL as estimated (J).

                b.  If the  furnace post  digestion spike recovery  is  not within  85-115%,
                    qualify the sample results < IDL as estimated (UJ).

         7.      If Method of Standard Additions (MSA)  is required  but  has  not been  done,
                qualify the data as estimated (J).

         8.      If any of the samples run  by MSA have not been spiked at  the appropriate
                levels, qualify the data as estimated (J).

         9.      If the MSA correlation coefficient is <0.995, qualify the data as estimated (J).


                                IX. ICP SERIAL DILUTION
 A.     Objective
        The serial dilution determines whether significant physical or chemical  interferences
        exist due  to sample matrix.

 B.     Criteria

        If the  analyte concentration is sufficiently high (concentration in the original sample
        is  minimally a factor of 50 above  the IDL), an  analysis of a 5-fold dilution must
        agree within  10% Difference (%D) of the original results.

C.     Evaluation Procedures

        I.      Check the  raw data and  recalculate the %D using the following equation to
               verify that  the dilution analysis results agree with results reported on Form IX.

                            %D .    I!z3   x 100


               Where.

               I  = Initial Sample Result
               S  = Serial Dilution Result (Instrument  Reading x 5)

       2.      Check the raw data for evidence of negative interference, i.e., results of  the
               diluted sample are significantly higher than the original sample.

D.     Action

       1.      When criteria are not met,  qualify the associated data as estimated (J).

       2.      If  evidence  of negative interference is  found,  use professional judgment to
              qualify the data.
                                              «3                                     7/88

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                            X.  SAMPLE  RESULT VERIFICATION
                                      ^

  A.     Objective

         The objective is to ensure that the reported quantitation results are accurate.

  B.      Criteria

         Analyte quantitation must be calculated according to the appropriate SOW.

  C.      Evaluation Procedures

         The  raw data should be examined to verify  the correct calculation oT sample  results
         reported by the laboratory.  Digestion and  distillation logs, instrument printouts, strip
         charts, etc. should be compared to the reported sample results.

         1.      Examine  the  raw  data  for any  anomalies  (i.e., baseline  shifts,  negative
                absorbances, omissions, legibility, etc.).

         2.      Verify  that there are no transcription or  reduction  errors (e.g., dilutions,
                percent solids, sample weights) on one or more samples.

         3.      Verify that results  fall  within the  linear range of the  ICP  (Form  XIII) and
                within the calibrated range for the non-ICP parameters.

         4.      Verify that sample results  are >5X  ICP IDL,  if ICP analysis results are used
                for As, TI. Se, or Pb.

         ("Jote:  When the laboratory provides both ICP and furnace results for an analyte in a
        sample and  the concentration is  >  ICP IDL, the  results can assist  in  identifying
        quantitation problems.

 D.     Action

        If there  are  any  discrepancies  found,  the  laboratory  may  be contacted by the
        designated representative to obtain  additional information that  could resolve any
        differences.   If a  discrepancy  remains  unresolved,  the  reviewer  may determine
        qualification of the data  is warranted.


                                 XI.  FIELD DUPLICATES

A.      Objective

        Field  duplicate  samples   may be  taken  and  analyzed as  an  indication of overall
        precision.  These analyses measure  both  field and lab precision; therefore, the results
        may have more  variability than  lab duplicates which measure only lab performance.
        It is also expected that soil duplicate results will have a greater variance than  water
        matrices due to difficulties associated with collecting identical field samples.

B.      Criteria

        There are  no review criteria for field duplicate analyses comparability.
                                                                                      7/88

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 C.      Evaluation Procedures       ,

        Samples  which are field  duplicates should  be  identified  using  EPA Sample Traffic
        Reports or sample field sheets.  The reviewer should compare  the results reported for
        each sample and calculate the Relative Percent Difference (RPD). if appropriate.

 D.     Action

        Any evaluation  of  the  field  duplicates  should  be  provided with the  reviewer's
        comments.


                  XII.  OVERALL ASSESSMENT OF DATA FOR A CASE

        It is appropriate for the data reviewer to make professional judgments  and express
 concerns and comments on the validity of the overall data for a Case.  This is particularly
 appropriate when there are several QC criteria out of specification.  The additive nature of
 QC factors  out of specification  is difficult to assess in an objective manner, but the reviewer
 has a responsibility to inform the  user concerning data quality and data limitation* in order to
 assist  that  user  in  avoiding  inappropriate  use  of the  data,  while not  precluding  any
 consideration of  the  data  at all.  If qualifiers other than those  used  in  this document  are
 necessary to describe or qualify the data,  it is necessary  to thoroughly document/explain  the
additional qualifiers used.  The data reviewer would be greatly assisted in this endeavor if  the
data quality objectives  were provided.  The cover form and  supplementary documentation
must be included with the  review.
                                             15                                      7/88

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                                   .  GLOSSARY A


                                Data  Qualifier Definitions


For the purposes of this document the following code letters  and associated definitions are
provided.

       U     -  The material  was analyzed for.  but was oot detected  above the level of the
               associated value.  The associated value is either the sample quantitation limit
               or the sample detection limit.                              ,

       J      -  The associated value is  an estimated quantity.

       R     -  The data are unusable.  (Note: Analyte may or may not be present.)

       UJ    -  The material was analyzed for. but was oot detected.  The associated value is
               an estimate and may be inaccurate or imprecise.
                                             16                                    7/88

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                                  GLOSSARY 0
                                Additional Terms
  Associated Samples
  AA

  Calibration Curve


  Case




 CCB



 CCS




 CCV



CLP

CRDL

CV

DPO

EMSL/LV



Field Blank
   Any sample related to a particular QC analysis.
   For example:
        For ICV, all samples run under the same
        calibration curve.

        For duplicate RPD, all SDG samples
        digested/distilled    of the same matrix.

   Atomic Absorption

   A  plot  of   absorbance  versus  concentration  of
  standards

  A finite,  usually predetermined  number of samples
  collected in a given time period for a  particular site.
  A Case consists of one  or more Sample Delivery
  Groups.

  Continuing Calibration  Blank  -  a  deionized water
  sample run every ten  samples designed to detect any
  carryover contamination.

  Contract Compliance  Screening  - process in  which
  SMO  inspects   analytical   data   for  contractual
  compliance and provides EMSL/LV, laboratories,  and
  the Regions with their  findings.

 Continuing Calibration Verification - a standard  run
 every  ten  samples designed  to  test  instrument
 performance.                             -  •

 Contract  Laboratory Program

 Contract Required Detection Limit

 Coefficient of Variation

 Deputy Project Officer

 Environmental   Monitoring   System   Laboratory/
 Las  Vegas  (P.O.    Box   15027,   Las   Vegas
 Nevada  89114)

 Field blanks are intended  to  identify contaminants
that  may   have  been   introduced  in   the field.
Examples    are    trip    blanks,    travel    blanks,
rinsate blanks, and decontamination  blanks.
                                       17
                                                                             7/88

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  Field Duplicate


  Holding Time


 ICB


 ICP

 ICS

 ICV


 Initial Calibration
 IRDA

 LCS

 MS




 MSA

 Post digestion Spike



QAC

RPD

RSCC

RSD

Serial Dilution
  A duplicate sample generated in the field, not in the
  laboratory.

  The   time  from  sample  collection  to  laboratory
  analysis.

  Initial Calibration Blank - first blank standard run to
  confirm the calibration curve.

  Inductively Coupled Plasma

  Interference Check Sample

  Initial Calibration Verification - first standard run to
  confirm the calibration curve.

  The  establishment  of a  calibration   curve   with
  the    appropriate   number   of   standards    and
 concentration range.   The  calibration  curve  plots
 absorbance  or   emission  versus  concentration   of
 standards.

 Inorganic Regional Data Assessment

 Laboratory Control Sample - supplied by EPA

 Matrix Spike - introduction of a known concentration
 of analyte  into a sample to provide information about
 the effect of the sample matrix on  the digestion  and
 measurement methodology.

 Method of Standard Addition

 The addition of a known amount of standard after
 digestion.     (Also  identified   as  analytical  spike,
 or spike, for furnace analyses.)

 Quality Assurance Coordinator

 Relative Percent Difference

 Regional Sample Control Center

 Relative Standard  Deviation

 A sample  run at  a  specific  dilution  to  determine
 whether  any   significant  chemical  or   physical
 interferences  exist due to  sample   matrix effects.
(ICP only)
                                        18
                                              7/88

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 SDG                            Sample  Delivery  Group -  defined  by  one of the
                                 following, whichever occurs first:

                                         case of field samples

                                         each twenty  field samples in a Case

                                         each  14-day calendar period  during which
                                         field   samples   in  a  Case  are   received,
                                         beginning  with  receipt of the first sample
                                         in the SDG.

SMO                           Sample Management Office

SOP                            Standard  Operating  Procedure

SOW                            Statement of Work
                                      19                                    7/88

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                                                                              Region
                          INORGANIC REGIONAL DATA ASSESSMENT
  CASE NO.	SITE
  LABORATORY 	   NO. OF SAMPLES/
                                             MATRIX 	
  SDG*	   REVIEWER (IF NOT ESD)
  SOW*	   REVIEWER'S NAME 	
  DPO: ACTION	FYI 	   COMPLETION DATE	
                               DATA ASSESSMENT SUMMARY

                                            ICP       AA       Hg         CYANIDE

  1.    HOLDING TIMES                    	   	   	    	

  2.    CALIBRATIONS                      	   	   	    	

  3.    BLANKS                            	   	   	    	
                                            1    .     —*——^—.       "       ~~    - . .  .
  4.    ICS                                 	

  5.    LCS                                 	   	

  6. .   DUPLICATE ANALYSIS	   	    	

  7.     MATRIX SPIKE                      	  	   	    	

  8.     MSA                                         	

  9.    SERIAL DILUTION                  	

 10.    SAMPLE VERIFICATION             	   	  	    	

 11.    OTHER QC                          	   	  	    	

 12.    OVERALL ASSESSMENT              	   	  	    	

    O «= Data had oo problems/or qualified due to minor problems.
    M = Data qualified due to major problems.
    Z = Data unacceptable.
    X - Problems, but do not affect data.
ACTION ITEMS:
AREAS OF CONCERN:
NOTABLE PERFORMANCE
                    U.S. Environmental Protection Agency
                    Region 5, Library (PL-12J)
                    77 West Jackson Boulevard,  12th Floor
                    I Kj>»j%^it.A. JJ  ^,/iy A *   -AA

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