c/EPA
         United States
         Environmental Protection
         Agency
            Region 5
            77 West Jackson Boulevard
            Chicago, Illinois 60604
EPA 905-R-96-005
NTS
September 1998
Development of Index of
Biotic Integrity Expeditions for
The Lake Agassiz Plain
Ecoregion

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                                      TABLE OF CONTENTS

Section	                                                                           Page
I.  List of Figures                                                                                iii
ii.  List of Tables                                                                                 vii
iii. Executive Summary                                                                            ix
iv. Acknowledgments                                                                              xi

1.0 INTRODUCTION                                                                           1

2.0 STUDY AREA                                                                              1

     Drainage Features                                                                            1
       Red River of the North Basin                                                               1
       Lake Agassiz  Plain Ecoregion                                                               3
     Historical Red River Valley Data                                                               5

3.0 MATERIALS AND METHODS                                                               5

     Establishing the Reference Condition                                                            5
       Criteria for Selecting Reference Sites                                                        5
       Fish Community Sampling Procedures                                                       7
       Habitat                                                                                  8
     Metrics                                                                                     8
       Scoring Modifications                                                                      47

4.0 RESULTS AND  DISCUSSION                                                                48

     Lake Agassiz Plain                                                                           48
     Headwater, Moderate, and Large Rivers                                                        50
     Minnesota and North  Dakota                                                                   51
     The Red River                                                                               52
     Variability                                                                                  53
     Reference Sites                                                                              53

5.0 REFERENCES                                                                              56

6.0 APPENDICES                                                                              61

     A. Documented species (current and historic) found in the Red River Basin (native, exotic, stocked) and those
       species collected from the Lake Agassiz Plain ecoregion during the 1993-1994 study period..

     B. Site specific Index of Biotic Integrity scores for the stations sampled in the Lake Agassiz Plain ecoregion.

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                                          LIST OF FIGURES
Figure
Number	                        Page

 1      Map delineating the major river systems of the Red River                                2
        Basin

 2      Map of the Red River Basin delineating the ecoregion's                                  4
        boundaries.

 3      Map of the Red River Basin showing historical sampling                                 6
        locations and sampling locations during the 1993-1994 study
        period.

 4      Maximum scoring lines used to determine trends in total                                 15
        number of species with increasing drainage area (DA) for the
        Lake Agassiz Plain ecoregion of Minnesota and North Dakota.

 5      Maximum scoring lines used to determine trends in the                                  18
        proportion of headwater individuals with increasing DA for the
        Lake Agassiz Plain ecoregion of Minnesota and North Dakota.

 6      Maximum scoring lines used to determine trends in the number                           19
        of benthic insectivore species with increasing DA for the Lake
        Agassiz Plain ecoregion of Minnesota and North Dakota.

 7      Maximum scoring lines used to determine trends in the                                  20
        proportion of round bodied suckers with increasing DA for the
        Lake Agassiz Plain ecoregion of Minnesota and North Dakota.

 8      Maximum scoring lines used to determine trends in the number                           23
        of minnow species with increasing DA for the Lake Agassiz
        Plain ecoregion of Minnesota and North Dakota.

 9      Maximum scoring lines used to determine trends in the                                  24
        proportion of large river individuals with increasing DA for
        the Lake Agassiz Plain ecoregion of Minnesota and North
        Dakota.

 10     Maximum scoring lines used to determine trends in evenness                              26
        with increasing DA  for the Lake Agassiz Plain ecoregion of
        Minnesota and North Dakota.

 11     Maximum scoring lines used to determine trends in the number                           28
        of sensitive species with increasing DA for the Lake Agassiz
        Plain ecoregion of Minnesota and North Dakota.
                                                  ill

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                               LIST OF FIGURES (CONTINUED)
Figure
Number	                        Page

 12     Maximum scoring lines used to determine trends in the                                  30
        proportion of tolerant individuals with increasing DA for the
        Lake Agassiz Plain ecoregion of Minnesota and North Dakota.

 13     Maximum scoring lines used to determine trends in the                                  32
        proportion of omnivore biomass with increasing DA for the
        Lake Agassiz Plain ecoregion of Minnesota and North Dakota.

 14     Maximum scoring lines used to determine trends in the                                  34
        proportion of insectivore biomass with increasing DA for the
        Lake Agassiz Plain ecoregion of Minnesota and North Dakota.

 15     Maximum scoring lines used to determine trends in the                                  36
        proportion of pioneer individuals with increasing DA for the
        Lake Agassiz Plain ecoregion of Minnesota and North Dakota.

 16     Maximum scoring lines used to determine trends in the                                  37
        proportion of piscivore biomass with increasing DA for the
        Lake Agassiz Plain ecoregion of Minnesota and North Dakota.

 17     Maximum scoring lines used to determine trends in the number                          39
        of individuals per meter with increasing DA for the Lake
        Agassiz Plain ecoregion of Minnesota and North Dakota.

 18     Maximum scoring lines used to determine trends in the                                  41
        proportion of simple lithophilic spawners with increasing DA
        for the Lake Agassiz Plain ecoregion of Minnesota and North
        Dakota.

 19     Maximum scoring lines used to determine trends in the                                  43
        proportion of individuals with DELT anomalies with
        increasing DA for the  Lake Agassiz Plain ecoregion of
        Minnesota and North Dakota.

 20     Maximum scoring lines used to determine trends in the                                  45
        proportion of subterminal mouth minnows with increasing DA
        for the Lake Agassiz Plain ecoregion of Minnesota and North
        Dakota.

 21     Maximum scoring lines used to determine trends in the number                          46
        of sucker species with increasing  DA for the Lake Agassiz
        Plain ecoregion of Minnesota and North Dakota.
                                                   IV

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                                  LIST OF FIGURES (CONTINUED)
Figure
Number        	                     —Page
 22     Plot of DA vs. IBI score for all sites sampled during the 1993-                           48
        1994 study period.

 23     Mean IBI scores for each river size classification in                                     49
        Minnesota, North Dakota, and the entire Lake Agassiz Plain
        ecoregion.

 24     Index of Biotic Integrity ranking (modified from Karr et al.                              50
        1986) for all sites sampled during the 1993-1994 study period
        for the Lake Agassiz Plain ecoregion of Minnesota and North
        Dakota.

 25     Longitudinal community trends in IBI scores for the Red                                 53
        River.

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                                         LIST OF TABLES

Table
Number	                         Page


  1      Total Index of Biotic Integrity (IBI) scores, modified scores                               10
        for the Lake Agassiz Plain ecoregion, integrity classes and
        attributes of IBI classification from Karr et al. (1986).

  2      Index of Biotic Integrity metrics used to evaluate headwater                               11
        streams (<200 mi2 DA) in the Lake Agassiz Plain ecoregion.

  3      Index of Biotic Integrity metrics used to evaluate moderate size                            12
        streams (200-1500 mi2 DA) in the Lake Agassiz Plain
        ecoregion.

  4      Index of Biotic Integrity metrics used to evaluate large rivers                              13
        (> 1500  mi2 DA) in the Lake Agassiz Plain ecoregion.

  5      List of headwater, benthic insectivore, and round bodied                                  17
        sucker species in the Lake Agassiz Plain ecoregion.

  6      List of minnow species in the Lake Agassiz Plain ecoregion                               22
        for evaluating  quality pool habitat.

  7      List of large river taxa found in the  Lake Agassiz Plain                                   22
        ecoregion, following Pflieger (1971) and Simon and Emery
        (1995).

  8      List of species found in the Lake Agassiz Plain ecoregion                                 27
        considered to be sensitive to a wide  range of environmental
        disturbances including water quality and habitat degradation

  9      List of species found in the Lake Agassiz Plain ecoregion that                             29
        are considered to be highly tolerant to a wide range of
        environmental disturbances including water quality and habitat
        degradation.

  10    List of omnivorous species found in the Lake Agassiz Plain                               31
        ecoregion.

  11    List of insectivorous  species  found in the Lake Agassiz Plain                              33
        ecoregion.

  12    List of pioneer species found in the Lake Agassiz Plain                                   35
        ecoregion.

  13    List of piscivorous species found in the Lake Agassiz Plain                                35
        ecoregion.

  14    List of species found in the Lake Agassiz Plain ecoregion that                             40
        are simple hthophilic spawners.
                                                    VI1

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                               LIST OF TABLES (CONTINUED)

Table
Number	                        Page


  15     List of subterminal mouth minnow species in the Lake Agassiz                          44
        Plain ecoregion.

  16     List of sucker species in the Lake Agassiz Plain ecoregion.                              44

  17     List of reference sites determined using fish community biotic                           55
        integrity for the Lake Agassiz Plain ecoregion.
                                                  vin

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                                        EXECUTIVE SUMMARY

The index of biotic integrity (IBI) has been used to evaluate the biological quality of rivers and streams in diverse
areas of the United States. The IBI  compares characteristics of lotic systems, termed "metrics", that represents the
structural and functional attributes of the fish community in three categories: 1) species richness and composition,
2) trophic structure, and 3) fish abundance and health.  Comparisons are made with  lotic systems possessing
unaffected or minimally affected  communities from ecologically similar areas.  Modifications of the metrics have
been made to account for regional or local characteristics of fish communities.  A combined project between the
USGS; USEPA, Regions V and VIII; Minnesota Pollution Control Agency; Minnesota DNR; and the North
Dakota Department of Health, Division of Water Quality targeted the multi-state  Lake Agassiz Plain (formerly the
Red River Valley) ecoregion for  IBI development and assessment.  All of the metrics have been modified for
application to this biologically young (since glaciation) system.  Several new metrics are proposed for various
stream sizes in the Lake Agassiz  Plain ecoregion. New  metrics include the evenness component of diversity,
trophic composition metrics based on biomass, a new definition for the omnivore feeding strategy, and as an
alternative metric, the number of cyprinid species with subterminal mouths. These new metrics are designed to
address the uniqueness of the  climate, hydrology, and biology of the region.
                                                   IX

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ACKNOWLEDGMENTS

The authors wish to express their appreciation to those individuals who made significant contributions to this
study.  The idea to conduct this study was conceived and initiated by Phil Johnson, formerly USEPA Region VIII,
Mike Ell, North Dakota Department of Health, and Patricia Bailey, Minnesota Pollution Control Agency.
Funding was provided by Susan Jackson, U.S. EPA, Headquarters, Office of Science and Technology.  We thank
the Minnesota Department of Natural Resources, specifically John Enblom and Konrad Schmidt for their sampling
and field expertise. We also thank Mike Feist and Nick Proulx who worked as interns for the MPCA during a
hard summer of sampling and showed great perseverance and dedication. Louise Hotka and Sandy Bissonette of
the MPCA also should be thanked for their contribution to the effort. Appreciation goes to the North Dakota
Game and Fish Department personnel, especially Lynn Schluetter, Andy Thompson,  BJ Kratz and Paul Seyer.
Pete Wax of the North Dakota Department of Health provided assistance in the field and Allen Johnson and Dan
Bain provided their expertise for computer related problems. Special gratitude goes to Dr. John Peterka,
Professor, North Dakota State University, for his time spent vouchering specimens.  The species list was provided
by Todd Koel, Ph.D. candidate, North Dakota State University.  Thanks to Willis  Mattison and local SWCD staff
for information about river conditions.  Dr. James Underbill and Dr. Jay Hatch of the Bell Museum assisted in
taxonomic identification and vouchering fish specimens collected in Minnesota. Maps were provided by Carrie
Bartz of the MPCA and personnel from  USGS.
                                                  XI

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                          Development of Index of Biotic Integrity Expectations
                   for the Lake Agassiz Plain Ecoregion of Minnesota and North Dakota
1.0 INTRODUCTION

Biological integrity is defined as "the ability to
support and maintain a balanced, integrated,
adaptive community of organisms having a species
composition, diversity, and functional organization
comparable to that of natural habitat of the region
(Kair and Dudley 1981).  One of the most widely
accepted analytical tools used to measure biological
integrity is the Index of Biotic Integrity (IBI).  The
IBI was developed in 1981 to evaluate  fish
community structure and function in midwestern
wadable streams and rivers (Karr 1981; Karr et al.
1986). This index relies on multiple variables
(termed "metrics") based on  community concepts, to
evaluate a complex biotic system. It incorporates
professional judgement in a systematic and sound
manner, but sets quantitative criteria that allows
determination of a continuum between  very poor and
excellent based on species  richness  and composition,
trophic and reproductive constituents, and fish
abundance and condition.  Since the metrics are
differentially sensitive to various perturbations (e.g.
siltation or toxic chemicals),  as well as various
degrees or levels of change within the range of
integrity, conditions at a site can be determined with
considerable accuracy.

The IBI has been viewed as a family of multi-metric
indices that needs to be modified for regional use
(Miller et al. 1988;  Simon and Lyons 1995).
Various permutations of the original IBI have been
developed for use for use in warmwater streams  in
northern sections of the Midwest (Simon and Lyons
 1995), Wisconsin (Lyons 1992), southern Minnesota
(Bailey et al. 1993), and southern Ontario (Steedman
 1988). However, the species depauparate systems of
the Hudson Bay  drainage have not been evaluated.

Because water within the Red River of the  North
Basin (referred to in the text as the  Red River Basin)
flows northward through prime agricultural lands in
the United States into Canada (Fig.  1), water quality
throughout the basin is an international concern;
particularly in the agriculturally impacted region of
the Lake Agassiz Plain.  Goldstein  et al. (1994)
emphasized multi-agency cooperation to develop an
IBI that could provide resource managers in the Red
River of the North Basin with a common tool to
evaluate water resource quality,  irrespective of
political boundaries.  The States of Minnesota and
North Dakota have recognized the importance of
developing numerical biological criteria to establish
benchmarks to assess water quality degradation in
this heavily impacted basin.  In addition, statewide
numerical biological criteria have been an important
component of the U.S. Environmental Protection
Agency's biocriteria program and monitoring and
assessment objectives (USEPA 19%). The U.S.
Geological Survey has recently completed an
assessment of water quality in the Red River Basin
as pan of the National Water Quality Assessment
(NAWQA) program (Stoner 1991).  Further
mention of the Red River Basin will refer only to
that portion within the United States.

Objectives:

The purpose of this report to outline the rationale for
potential IBI metrics to evaluate the Lake Agassiz
Plain ecoregion (formerly the Red River Valley
ecoregion) within the Red River Basin. This report
includes specific  Index of Biotic Integrity criteria
including the development of metrics and maximum
scoring lines, to delineate areas of high quality
within the Lake Agassiz Plain ecoregion of the Red
River Basin. We suggest that the criteria presented
in this document  are a "first attempt" to evaluate
rivers and streams in the Lake Agassiz Plain
ecoregion.

2.0 STUDY AREA

Drainage Features

Red River of the North Basin

The Red River Basin drains 17,500 mi2 in northwest
Minnesota, 21,000 mi2 in eastern North Dakota,  and
800 mi2 in northeastern South Dakota (Renard et al.
1986).  The Red River is the major drainage unit  in
the basin (Fig.  1). The river meanders northward
for 394 miles to the United States-Canadian border
(IRRPB 1995).  The mean annual flow of the Red
River increases from 519  cfs at its source at the
confluence  of the Bois de  Sioux and Oner Tail
Rivers  (Renard et al.  1986)  to 3386 cfs at the United
States-Canadian border (IRRPB  1995). Much of the
flow occurs during the spring and early summer
months when snow melt and heavy rains can cause

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 Lake Agassiz Plain
          100-
                                                  MANITOBA
Ban from U
1 2.000.000. 107;
projection Stand
4S°40'. oantral IT
                SCALE
                     100 KILOMETERS
                   Figure  1.  Major rivers In  the Red River of  the North  Basin.

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                                                                   Minnesota-North Dakota Ecoregion
severe flooding.  The gradient gradually declines
from  1.3 ft/mi at Wahpeton-Breckenridge to 0.2
ft/mi  at the United States-Canadian border (Stoner et
al. 1993).

About 75% of the Red River's flow orginates from
tributaries in Minnesota (Tornes and Brigham 1994).
The largest of these tributaries, the Red Lake River,
enters into the Red River at East Grand Forks.
With  an mean annual flow of 1110 cfs, the Red
Lake  River contributes almost 1/3 of Red River's
flow (Renard et al. 1983). Other major tributaries
of the Red River in Minnesota from south to north
include the Mustinka, Otter Tail, Buffalo, Wild
Rice,  Marsh, Sandhill, Snake, Middle, Tamarac,
Two,  and Roseau Rivers.

Tributaries to the Red River in North Dakota
contribute substantially less flow.  The largest
tributary to the Red River in North Dakota is the
Sheyenne River with a mean annual flow of  184 cfs.
Other major tributaries of the Red River in North
Dakota from south to north include the Wild Rice,
Maple, Rush, Elm, Goose, Turtle, Forest, Park, and
Pembina Rivers.

Lake  Agassiz Plain Ecoregion

The study area was once entirely inundated by an
immense glacial  lake known as Lake Agassiz. Lake
Agassiz once covered over 22,000 mi2 in Minnesota,
South Dakota and North Dakota (Waters 1977).
During the 12,000 years since the last glacial retreat
the waters of the basin have warmed, erosion has
cut new and deeper stream channels, drainage
patterns have changed,  and vegetation has changed.
Many of these changes came about or  were
accelerated by human activities. The rich glacial
lake sediments left behind by Lake Agassiz were
highly desirable for agriculture. The native
vegetation, mainly tall grass prairie, was cleared for
the production of wheat, other small grains, corn,
potatoes, soybeans, and  vegetables.  The land was
drained and treated with fertilizers, herbicides, and
pesticides to increase productivity.  Currently, over
90% of the land  in counties bordering  the Red River
in Minnesota have  been cultivated (MPCA 1994).
poorly drained,  a massive system of drainage ditches
was built to bring the land into production. In
Minnesota alone, over 7,400 mi of drainage ditches
were created in  the Red River Valley  since the late
1800's  (USGS  1974). To further move water
rapidly off the land, many streams were channelized
and a system of flood control impoundments were
built to control flooding. These extensive channel
modifications along with agricultural run off and
wastewater inputs from industrial and municipal
sources altered the physical, chemical, and
biological makeup of rivers and streams in the Red
River basin.

 Ecoregions (Omernik 1987) were used to determine
the geographic boundaries of different areas within
the basin.  Omernik (1987) mapped the ecoregions
of the conterminous United States from maps of
land-surface form, soil types, potential natural
vegetation, and land use. Ecoregions  were defined
as areas of homogeneous ecological systems or areas
that have the potential (if undisturbed) for similar
biological communities (Omernik and  Gallant 1988).
The Lake Agassiz Plain ecoregion was the only
ecoregion that was entirely included in the Red
River Basin.  However, four additional ecoregions
surrounded the Lake Agassiz Plain ecoregion  and
comprised the outer portion of the basin (Fig. 2).
The other four ecoregions in the basin were the
North Central Hardwood Forests, Northern
Minnesota Wetlands, Northern  Glaciated  Plains, and
Northern Lakes  and Forests.

Because of Lake Agassiz's influence on the basin,
the land in the central portion of the basin was
significantly different than the land at  the outer
edges and as  a result, so was the condition of the
water resources. Headwater reaches of most major
tributaries to  the Red River originated  in the outer
ecoregions (Fig. 1 and 2). The majority of these
tributary streams were higher in gradient, had
definitive geomorphological units (i.e., riffles,
pools, and runs), and were dominated  by course
gravel or cobble substrates.  As tributary streams
entered  the Lake Agassiz Plain ecoregion,  their
gradient decreased and there was a shift in stream
morphology to slow moving, meandering  runs.
Since the soils of the Red River Basin were naturally

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 Lake Agassiz Plain
        49°
                                        98°    MANITOBA
Base Irom U S Geological Survey digital data
1 2.000,000, 1972  Albers Equal-Area Conic
projection Standard parallels 29°30  and
45°40'. central meridian  -97°00
              SCALE

                50


                   100 KILOMETERS
          EXPLANATION

          Red River Valley

          Northern Glaciated Plains

          Northern Minnesota Wetlands

          North Central Hardwood Forest

          Northern Lakes and Forests
  Lake}
Traverse
                      Figure 2.  Ecoregions of the Red River of the North Basin
                                  (modified from Omernik, 1987).

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                                                                  Minnesota-North Dakota Ecoregion
The fine alluvial deposits of Lake Agassiz were
reflected in the substrate of the rivers and streams
such that sand, silt, and clay dominate. These
physical changes associated with Lake Agassiz
affected water chemistry by contributing to an
increase in turbidity, conductivity, and total
suspended solids.
than those in the east.  Of the 75 species found in
the major rivers of the basin, 51 were present in the
western half; whereas, 71 were found in the east.
Species richness was reported to be affected by
drainage area (DA) and the number of ecoregions a
river flows through (Goldstein 1995).
Historical Red River Valley Data

Physical and chemical characteristics of water
resources within the Red River Valley have been
summarized in reports on ground and surface water
hydrology (Stoner et al. 1993; Maclay et al. 1965;
Maclay et al. 1967;  Maclay et al. 1969(a); Maclay
et al. 1969(b); Maclay et al. 1972; Winter et al.
1967; Winter et al. 1970; Winter et al.  1984),
hydrogeology (Ruhl 1986; Siegel 1981), nutrient and
sediment transport ( Tornes 1986; Tornes and
Brigham 1994), and surface water quality (MFCA
1969; MPCA 1977; MPCA 1994). Biological
communities of the Red River basin have been
summarized in numerous reports including surveys
of fish  (Enblom 1982; Renard et al.  1983; Renard
et al. 1986; Hanson et al. 1984; Neel 1985; Peterka
1978; Peterka 1991), macroinvertebrates (Neel
1985; Hanson et al. 1984),  mollusks (Dawley 1947;
Cvancara  1970; Cvancara 1983) and aquatic plants
(Renard et al.  1983).

Renard et al. (1986)  conducted fish community
assessments at 14 locations on the Red River.
Thirty six species were collected from 12 families.
Carp comprised over 50% of the total catch by
weight, while game fish were not common in the
collection. The most common game fish species
were channel catfish (8.2%) and walleye (3.2%).
Renard et al. (1983)  surveyed the fish community at
8 sites on the Red Lake River in Minnesota, the
largest tributary of the Red River. Thirty eight fish
species from  13 families were collected during the
survey. Of the 6 rivers in North Dakota that
Peterka (1991) surveyed, Park River had the
greatest number (18) offish species. Goldstein
(1995) summarized historical fish community data
from the Red River basin and reported that western
tributaries of the Red River supported fewer species
3.0 MATERIALS  AND  METHODS

Establishing the Reference Condition

Reference conditions define the physical, chemical,
and biological expectations of minimally impacted
rivers and streams (Hughes et al. 1982; Whittier et
al. 1987).  Properly defined reference conditions
provide a reasonable benchmark to measure the
degree of water quality degradation (Hughes and
Omernik 1981; Hughes et  al. 1986).

To determine the reference condition for the Lake
Agassiz Plain ecoregion, 111 locations were
sampled throughout the ecoregion during June
through August 1993-1994 (Fig. 3). These  sites
were chosen because they were thought to represent
least impacted areas of the basin and were
considered to be candidate reference sites.  In
addition, select data from past MDNR fisheries
surveys conducted at 102 sites was  also added to the
database. Only those MDNR surveys that contained
information on the entire fish communty and that
used electrofishing methods to capture fish were
included in the analysis.

Criteria for Selecting Reference Sites

Sites that approximated the regional reference
condition were considered  candidate reference sites.
These sites possessed a well  balanced, adaptive
aquatic community and chemical and physical
characteristics indicative of presettlement times.  We
used the following criteria  to help distinguish a
candidate reference site from other  sites:  1) The site
had a natural stream geomorphology.  The stream
channel had not been altered by dredging or
channelization; 2) There was a continuous riparian
area along the reach extending laterally about three
times the channel width.  Land use  was consistent

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Lake Agassiz Plain
            EXPLANATION
          •  1993-1994 Sites
          A  Historical Sites
                        100 Kiometers
         Figure 3. Map of the Red River Basin indicating historical sample locations
                   as well as sample locations during the 1993-1994 study period.

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                                                                   Minnesota-North Dakota Ecoregion
laterally and soils and vegetation are undisturbed; 3)
The vegetation was undisturbed within the riparian
area. Woody vegetation was not logged or removed.
Grasses were not cut, burned, or treated with
herbicides.  If vegetation was altered historically,
the plant community within the  riparian area should
have had sufficient time to grow back to its
predisturbed condition;  4) The  stream segment had
not been stocked with forage or game fish species;
5) There was no point sources or in-flowing springs,
ditches, or drainage canals;  6)The reach
geomorphology was consistent with segment
geomorphology; 7) There was no stream habitat
improvements (wing dams, rip rap, etc.);  8) The
site was not snagged. All woody debris remained in
the stream unless moved by  natural processes;  9)
There were no dams or diversions upstream or
downstream within two meander cycles or two
replications of major geomorphological units; 10)
There were no bridges upstream within two meander
cycles or two replications of major
geomorphological units.

Few sites in the  Lake Agassiz Plain met all of these
criteria because natural, undisturbed land cover in
the ecoregion was  almost nonexistent. Therefore,
the candidate reference sites could not be considered
pristine or undisturbed. Rather, these sites
represented the best available conditions given  the
anthropogenic impacts, channelization, and cultural
eutrophication that had occurred in the Lake Agassiz
Plain ecoregion.

The selection of candidate reference site locations
was based on information from local resource
managers, historic fish collections by  the Minnesota
Department of Natural Resources and the North
Dakota Department of Health, and on-site
reconnaissance.  Obtaining an adequate spatial
coverage of the ecoregion also helped determine the
site locations.

Whenever available, candidate reference sites
included natural areas, parks (federal, state, county,
and local), exceptional designated streams, and
historical sampling locations. Since ditching
occurred at most of the headwater sites in the
ecoregion, we selected sites that indicated recovery
from channelization or potential non-point source
pollution areas and had a stable riparian buffer on
the shoreline.  When a series of point source
dischargers were located on a river, effort was made
to sample upstream of the discharger or to search
for areas of recovery between dischargers.

When impoundments or other physical habitat
alterations had been  imposed on a river, the best
suitable, stable site was used as a sampling location.
"Suitable" in these instances were sites considered
free from the adverse affects of impoundments or
habitat alterations and those sites which posed no
threat of injury to personnel. In areas where
sampling could not be accomplished due to lack of
access downstream of the physical structure,
candidate reference sites  were located upstream of
the dam away from the immediate influence of the
pooled portion. Likewise, bridges were usually
sampled on the upstream side, away from the
immediate vicinity of the structure and latent bridge
construction effects.  If downstream sampling was
conducted because of habitat considerations,
sampling began at least 50 m downstream of the
bridge.

Fish Community Sampling Procedures

All sites were rigorously  sampled in order to get
representative, quantitative estimates of species
richness and biomass.  Sampling was conducted in
all river size classes  in the Lake Agassiz Plain
ecoregion from headwater streams which we defined
as those streams  < 200 mi2 DA, to the large rivers
with DA > 2,000 mi2. The reach length of each
site was determined by multiplying the  mean stream
width by 15 with a maximum reach length of 500 m.
Therefore, stream reach length  varied with  stream
size.  Sites ranged in length from 50 m at some
headwater sites to 500 m  at large river sites.

Sampling took place during the  summer to take
advantage of the low and stable  flow conditions
usually found during this  time of the year.  Karr et
al. (1986) found  that low to moderate stream flows
were preferred and the relatively variable flow
conditions of early spring and late autumn should be
avoided.  Unfortunately, 1993 and 1994 were
somewhat atypical because water levels remained
high in the Lake  Agassiz  Plain throughout the

-------
 Lake Agassiz Plain
summer months.

Gear selection was dependent on stream size,
velocity, substrate, and depth.  However, only one
electrofishing gear type was used at each site to
collect a representative sample (Jung and
Libosvarsky 1965; Ohio EPA 1989).  A generator
powered backpack electrofishing unit or a T&J
pulsed-DC generator (300 V,  1750 watts) mounted
in a Coleman Sport-canoe, floated in a Sport-Yak,
or attached to a long-line was used to sample fish at
headwater and moderately sized wadable streams
(200-1500 mi2 DA).  Large river sites (> 1500 mi2
DA) were sampled using a long line, sport-yak, or
boat electrofisher (see Ohio EPA 1989 or USEPA
1988 for discussion of gear).  Sampling occurred
along both shorelines in streams > 5 m width or
followed a serpentine pattern on both shores for
streams <5 m width.

At each site, we attempted to collect all fish that
were encountered. Adult and juvenile specimens
from each site were counted and identified to species
using the taxonomic keys of Becker (1983), Gerking
(1955), and  Trautman (1981). Cyprinid taxonomy
followed Mayden (1989). Smaller and more difficult
to identify taxa were preserved for laboratory
examination and identification. Young-of-the-year
fish (less than 20 mm in length) were not included in
the analysis.  Early-life stages may exhibit high
initial mortality (Simon  1989) and are often difficult
to collect with gear designed for larger fish
(Angermeier and Karr 1986).  Specimens >  20 mm
total length (TL) were easily collected using our
gear.

All fish were examined for the presence of gross
external anomalies. Incidence of these anomalies
was defined as the presence of externally visible
morphological anomalies (i.e. deformities, fin or gill
erosion, lesions/ulcers and rumors). Specific
anomalies included: fin rot; pugheadedness;
Aeromonas  (causes ulcers, lesions, and skin growth,
and formation of pus-producing surface lesions
accompanied by scale erosion); dropsy (puffy body);
swollen eyes; fungus; ich; curved spine; swollen-
bleeding mandible or opercle.  Incidence was
expressed as percent of anomalous fish  among all
fish collected.  Incidence of occurrence was
computed for each species at each station. Hybrid
species encountered in the field (e.g. hybrid
centrarchids, cyprinids) were recorded on the data
sheet, and when possible, potential parental
combinations recorded.
A general site evaluation for each sampling location
was conducted using the Qualitative Habitat
Evaluation Index (QHEI), (Ohio EPA 1989; Rankin
1989), and Rapid Bioassessment Protocol
(RBP)(Plafkin et al.1989). The QHEI and RBP
measured important: features of the habitat not only
in the channel but also in the surrounding
environment. Scoring included information on
substrate composition, instream cover, channel
morphology, riparian zone and bank erosion, and
pool and riffle quality. Physical-chemical parameters
were recorded for each site including; dissolved
oxygen, pH, temperature, and specific conductivity,
as well as general water chemistry (e.g. hardness
alkalinity, nitrogen, phosphorus) for North Dakota
sites. Equipment used for water quality analysis
varied by each agency conducting the surveys,  but
each meter was used following the specifications of
the manufacturer.

Metrics

The interpretation of the original IBI scoring system
(Karr et al. 1986) and the scoring system developed
for the Lake Agassiz Plain ecoregion is provided in
six narrative categories (Table 1). Numerical
category boundaries for the Lake Agassiz Plain were
determined by comparison of the IBI scores and fish
community attributes with the narrative category
descriptions provided by Karr (1981).

Below  is an explanation of each metric used in  the
calibration of the Index of Biotic Integrity  for the
Lake Agassiz Plain.  Metric selections were based
on the  recommendations of Goldstein et al. (1994).
Each metric was calibrated using data collected
during the 1993-1994 study period and appropriate
historical data. The proportion of DELT anomalies
and catch-per-unit-effort were  based on very little
historical information since this information was
seldom available in the historical records.  Only data

-------
                                                                   Minnesota-North Dakota Ecoregion
collected during the 1993-1994 study period was
presented in the Results and Discussion.

Several of the metrics were drainage size dependent
and required calibration to determine numerical
scores (Tables 2-4). Drainage size effects were
determined by evaluating trends in species or
proportions of individuals with increasing (log
transformed) DA.   Maximum scoring lines were
drawn following the procedure of Fausch et al.
(1984) and Ohio EPA (1987).  Scatter plots of
individual metrics were first evaluated for basin
specific patterns. The trisection method was used to
show the maximum scoring lines. This required that
the uppermost line be drawn so that  95 %  of the  data
points were beneath the line. The other two lines
were drawn so the remainder of the  area beneath the
95th percentile line was divided into three equivalent
areas. If no relationship with increasing DA was
observed, the maximum scoring lines either leveled
off at the point where no additional increases were
exhibited or horizontal plots were delineated
indicating no increase with DA.  Deviations from the
Fausch et al. (1984) procedure were necessary when
outliers were present or when it was determined that
the metric did not respond in a linear fashion.  This
was the the case with the piscivore metric where  a
high and  low proportion of piscivorous species
indicated an unbalanced fish community.
Differentiation between headwater, moderate, and
large river sites was determined by searching  for
bimodal patterns in the basin specific data set  plots.
The  tails  of distribution of the data were not
significant.  However, the point where the data
differentiated into two distinct peaks suggested that
the transition between headwater and moderate sized
streams was at 200 mi DA and between moderate
sized streams and large rivers was at 1,500 mi2 DA.

-------
 Lake Agassiz Plain
Table 1. Total Index of Biotic Integrity (IBI) scores, modified scores for the Lake Agassiz Plain ecoregion,
integrity classes, and attributes of IBI classification from Karr et al. (1986).

Karr IBI  Lake Agassiz  Integrity
 score        Plain        class          Attributes

58-60       51-60       Excellent       Comparable to the best situation without human disturbance; all
                                        regionally expected species for the habitat and stream size,
                                        including the most intolerant forms, were present with a full array of
                                        age (size) classes; balance trophic structure.

48-52      41-50       Good           Species richness was somewhat below expectations, especially due to
                                        the loss of the most intolerant  forms; some species were present
                                        with less than optimal abundances or size distributions; trophic
                                        structure shows some signs of stress.

40-44      31-40       Fair            Signs of additional deterioration included loss of intolerant forms,
                                        fewer species, highly skewed trophic structure (e.g. increasing
                                        frequency of omnivores and other tolerant species); older age
                                        classes of top predators were rare.

28-34      21-30       Poor           Dominated by omnivores, tolerant forms, and habitat generalists;
                                        few top carnivores; growth rates and condition factors commonly
                                        depressed; hybrids and diseased fish were often present.

 12-22       12-20       Very Poor      Few fish were present, mostly introduced or tolerant forms; hybrids
                                        were common; disease, parasites, fin damage, and other anomalies
                                        regular.

0                                      No fish
                                                     10

-------
                                                               Minnesota-North Dakota Ecoregion
Table 2. IBI metrics used to evaluate headwater streams* ( < 200 mi2 DA) sites in the Lake Agassiz Plain
ecoregion.
Category
Species richness and
composition



Trophic composition

Reproductive guild
Functional guild

Fish abundance and
condition

Scoring Classification
Metric 5 3
Total number of species Varied with DA
Evenness >0.8 >0. 6 and _<0.8
Number of minnow species Varied with DA
% Pioneer individuals' Varied with DA
% Headwater individuals > 50 > 25 and _<_50
% Omnivore biomass1 <33 _>_33 and <66
% Insectivore biomass1 > 60 > 30 and _<_60
% Simple lithophilic1 > 60 > 30 and j< 60
% Tolerant individuals < 3 1 >3 1 and < 62
Number of sensitive species Varied with DA
Number of individuals per Varied with DA
meter
% DELT <1 .>_! and < 4
1
^0.6


-<25
>66
^30
<30
^62


-4
*Headwater metrics discussed individually in text.
1 Special scoring procedures were required when < 25 individuals are collected at the site or the number of
species metric is scored a " 1" .

-------
 Lake Agassiz Plain
Table 3. IBI metrics used to evaluate moderate size streams* (200-1500 mi2 DA) sites in the Lake Agassiz
          Plain ecoregion.
Category
Species richness and
composition


Metric
Total number of species

Evenness
Number of minnow species
5
Varied

>0.8
>7
Scoring Classification
3
with DA

> 0.6 and ^0.8
> 4 and < 7
1


^0.6
<4
 Trophic composition
                          Number of benthic insectivore    > 7
                          species
% Piscivore biomass1
                                               >4and <7
> 20 and < 30   > 10 and j< 20,
                > 30 and < 40
                                   <4
< 10 and >40


Reproductive guild
Functional guild

Fish abundance and
condition

Alternative metrics



% Omnivore biomass1
% Insectivore biomass1
% Simple lithophilic spawners1
% Tolerant individuals
Number of sensitive species
Number of individuals per
meter
% DELT
% Headwater individuals
% Pioneer individuals
% Subterm. mouth minnows
Number of sucker species
<33 ^33 and <66
> 60 > 30 and j< 60
> 60 > 30 and _< 60
<31 _>31 and <62
Varied with DA
Varied with DA
< 1 _>_! and <4
>50 >25and<50
Varied with DA
>20 >10and_<.20
> 4 > 2 and ^4
^66
^30
^.30
>_62


^4
.<25

<.10
<2
 *Moderate sized stream metrics are discussed individually in text.
 1 Special scoring procedures were required when < 50 individuals are collected at the site or the number of
 species metric is scored a "1".
                                                   12

-------
                                                                Minnesota-North Dakota Ecoregion
Table 4. IBI metrics used to evaluate large river* (> 1500 mi2 DA) sites in the Lake Agassiz Plain
ecoregion.
 Category
 Metric
                                                                   Scoring Classification
                                                        5                   3              1
 Species richness and       Total number of species
 composition
                          Evenness

                          % Large river individuals

                          % Round bodied suckers

 Trophic composition       % Piscivore biomass'
  Reproductive guild

  Functional guild
  Fish abundance and
  condition
  Alternative metrics
                               >0.8
                              Varied with DA
                               >40
                > 8 and  <_16       ^8

                >0.6and <0.8    <0.6
                >20and  <40
                               >20 and <30   > 10 and <20,
                                              >30 and <40
% Omnivore biomass1            < 33

% Insectivore biomass1           >60

% Simple lithophilic spawners1    > 60
% Tolerant individuals
Varied with DA
Number of sensitive species      > 6

Number of individuals per        Varied with DA
meter
_X33 and < 66

> 30 and j< 60

> 30 and j<_60



> 3 and < 6
% DELT

% Pioneer individuals

Number of sucker species
                                                         <1
                > 1 and < 4
Varied with DA

> 4             > 2 and
                                       and >40
>:66

00

j<30



<3
                    >4
                                                                                            <2
 *Large river metrics are discussed individually in the text.
 1 Special scoring procedures were required when  < 50 individuals are collected at the site or the number of
 species metric is scored a " 1".

-------
 Lake Agassiz Plain
Metric 1. Total Number of Fish Species (All Streams and Rivers)
Impetus

This metric was used for all stream sizes  The
metric was considered to be one of the best for
determining stream condition due to a correlation
between high quality resources and the number of
fish species in warmwater assemblages (Ohio EPA
1987; Davis and Lubin 1989; Plafkin et al. 1989;
Simon 1991). The rationale for this metric was
based on the observation that the number of fish
species increased directly with environmental
complexity and quality (Karr 1981; Karr et al.
1986).

Non-native species were included for the Lake
Agassiz Plain ecoregion. Although the number of
non-native or introduced species may be indicative
of a loss of integrity (Karr et al. 1986; Ohio EPA
1989), they were included to help discern
differences between sites that were at the lower
levels of biotic integrity. The only commonly
occurring non-native species found in the basin was
the common carp Cyprinus carpio and it was
introduced over one hundred years ago.

The difference between what was defined as
headwater and moderate sized streams in the Lake
Agassiz Plain ecoregion was made using information
from this metric.  We separated headwater and
moderate sized streams at 200 mi2 DA.  The number
of species was strongly correlated with DA at
headwater and moderate sized stream sites up to
1500 mi2 DA (Fig. 4).  There was not a DA
relationship for large river sites (> 1500 mi2 DA)
because the number of species reached an
asymptote.

This metric was used in deciding the scoring
modifications (See Scoring Modifications, last
section).  When the number of species  at a site was
extremely low (score equals 1) special scoring was
needed  because the scores for the proportional
metrics did not  reflect the true quality of the
resource.
                                                   14

-------
                                                      Minnesota-North Dakota Ecoregion
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-------
 Lake Agassiz Plain
Metric 2. Proportion of Headwater Species (Headwater Streams)
         Number of Benthic Insectivores (Moderate Sized Streams)
         Proportion of Round-bodied Suckers (Large Rivers)
Impetus

The number of darter species was included in Karr's
(1981) IB1.  Darters are insectivorous, habitat
specialists, and sensitive to physical and chemical
environmental disturbances (Page 1983; Kuehne and
Barbour 1983). Darters are indicators of a quality
resource, and are generally found in riffle habitats.
However, few darter species occur in streams of the
Lake Agassiz Plain ecoregion.  For this reason  we
replaced the number of darter species metric with
three separate metrics (Fig. 5, 6 and 7).

Proportion of Headwater Species

Ohio EPA (1987) and Simon (1991) demonstrated
that certain fish assemblages inhabited headwater
streams (50 km2 DA).  Ohio EPA (1987) and Simon
(1990) designated nine species as headwater taxa.
Five species found in the Lake Agassiz Plain were
designated as headwater taxa (Table 5).  Headwater
fish species were associated with stable flow
conditions,  permanent habitat, low environmental
stress and higher biological  integrity (Ohio EPA
 1987; Simon 1990).

Because the Lake Agassiz Plain ecoregion
experiences a wide range of climatic conditions,
intensive drought and flooding cycles may occur.
Prior to the first year of this study, the Red River
Valley and most of North Dakota suffered through a
prolonged drought. However, during the study
water levels were high.  The abundance and
composition of headwater species can be directly
 related to these natural cycles.

 Number of Benthic Insectivore Species

 The minnows, suckers,  and  madtoms were benthic
 insectivores that functionally occupied the same type
 of niche as the darters (Ohio EPA 1987; Simon
 1991). Their inclusion allowed a greater degree of
 sensitivity in evaluating streams  that naturally had
 few darter species. We designated 19 species to be
benthic insectivores (Table 5).  Except for blacknose
dace, which may be behaviorally plastic (Leonard
and Orth 1986), the remainder of the species were
found in riffle habitats usually over clean gravel
substrates.  An increase in benthic insectivore
species was correlated with increased biotic
integrity.

Benthic insectivores were absent  at a significant
number of headwater sites.  This was a deciding
factor for using separate metrics  for each drainage
size.  This metric asymptoted at moderate stream
sizes and declined at the largest DA's (Fig. 6)

Proportion of Round-bodied Suckers

The number of sucker species was used in Karr's
(1981) IBI.  Unlike smaller benthic insectivores
which were difficult to collect in  large rivers,
suckers were effectively sampled with electrofishing
gear and comprised a significant component  of the
large riverine fish fauna.  Due to their long life
cycles (10-20 years), suckers provided a long term
assessment of past environmental conditions. Most
sucker species were intolerant to  habitat and water
quality degradation (Phillips and Underhill 1971;
Karr et al. 1986; Trautman 1981; Becker 1983).
Their feeding and reproductive requirements were
sensitive to turbidity and marginal to poor water
quality resulting in sensitivity at the higher end of
environmental quality.

We applied the proportion of round-bodied suckers
for DA > 1500 mi2. Round-bodied suckers  include
members of the sucker genera Minytrema.
Hypentelium. Moxostoma. Cycleptus. and Erimyzon
(Table 5). We did not include members of the
sucker genus Catostomus since members of  this
genus are able to tolerate a  wide  variety of
environmental perturbations.  Of the 9 sucker
species present in the Red River  Basin, five  were
considered round bodied (Table 5). The proportion
of round-bodied suckers did not show any
relationship with DA for large rivers (Fig. 7).
                                                    16

-------
            Minnesota-North Dakota Ecoreeion
Table 5. List of headwater, benthic insectivore, and round-bodied sucker species in the Lake Agassiz Plain
ecoregion.
Common name
Northern hogsucker
Silver redhorse
Golden redhorse
Shorthead redhorse
Greater redhorse
Silver chub
Bigmouth shiner
Sand shiner
Blacknose dace
Longnose dace
Northern redbelly dace
Finescale dace
Pearl dace
Trout-perch
Rainbow darter
Iowa darter
Least darter
Johnny darter
Logperch
Blackside darter
Stonecat
Tadpole madtom
Brook stickleback
Scientific name Headwater
species
Hypentelium nigricans
Moxostoma anisurum
M- erythrurum
M- macrolepidotum
M- valenciennesi
Macrhybopsis storeriana
Notropis dorsalis
N. stramineus
Rhinichthys atratulus X
R. cataractae
Phoxinus eos X
Phoxinus neogaeus X
Margariscus margarita X
Percopsis omiscomaycus
Etheostoma caeruleum
E. exile
E. microperca
E. nigrum
Percina caprodes
P. maculata
Noturus flavus
N. gyrinus
Culaea inconstans X
Benthic insectivore Round-bodied
species sucker species
X X
X X
X X
X X
X X
X
X
X
X
X



X
X
X
X
X
X
X
X
X

17

-------
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            Minnesota-North Dakota Ecoregion
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                                                                                       s
                                                                                       3'
                  Drainage Area  (Sq.  Mi.)
Figure 7:  Maximum scoring lines for determining trends in the proportion of round-bodied suckers with

increasing drainage area for the Lake Agassiz Plain ecoregion.

-------
                                                                 Minnesota-North Dakota Ecoregion
Metric 3. Number of Minnow Species (Headwater and Moderate Sized Streams)
          Proportion of Large River Individuals (Large Rivers)
Impetus

The number of minnow species replaced the number
of sunfish species to evaluate pool habitat quality
(Karr 1981).  Sunfish were not common in the Lake
Agassiz Plain. Instead, a diverse minnow
community occupied pool habitats (Table 6).  Since
minnows were often difficult to collect in large
rivers, another metric was substituted for the
original sunfish metric proposed by Karr (1981).
Although not a complete replacement for the
evaluation of pool habitat, the lack of sunfish species
in the Lake Agassiz Plain ecoregion facilitated the
substitution of the proportion of large river
individuals.

Number of Minnow Species

Eleven minnow species occurred in headwater and
moderate sized streams.  Species  including the
hornyhead chub Nocomis biguttatus. sand shiner
Notropis stramineus. and rosyface shiner N. rubellus
were examples of minnows that occurred in high
quality areas. Species such as creek chub  Semotilus
atromaculatus. fathead minnow Pimephales
promelas. and golden shiner Notemigonus
crysoleucas were tolerant to both  chemical
degradation and stream desiccation.  High numbers
of minnow species  corresponded with higher
biological integrity.

Because many minnow species occupied slower
moving pool habitats they may have been exposed to
areas  that acted as  "sinks" for the accumulation of
toxins and silt. Thus, this metric  measured
degradation of rock substrates (i.e. gravel and
boulder)(Hughes  and Gammon 1987), instream
cover (Pflieger 1975; Trautman 1981), and the
associated aquatic macroinvertebrate community
which was an important food resource (Forbes and
Richardson 1920; Becker 1983). Minnows were an
important component of the aquatic  community to
measure because  they were wide-ranging and they
were susceptible  to collection gear in most streams
and rivers of Minnesota and North Dakota. The
number of minnow species asymptoted at moderate
sized streams (Fig. 8).

Proportion of Large River Individuals

Just as darters, madtoms, sculpins, and  other benthic
insectivores were characteristic of small streams, so
certain species were commonly found in large river
habitats (Table 7). Pflieger (1975), Burr and
Warren (1986), Simon (1992), and Simon and
Emery (1995) found that a characteristic fish faunal
assemblage was apparent in large and great river
habitats. Simon  (1992) found that certain fish
species such as sturgeon, gar, river shiner,
carpsuckers, buffalo, channel catfish, and some
chubs appeared at DA's about 2,000 mi2. The
number of large river species in the Lake Agassiz
Plain ecoregion was expected to predominate in >
1,500 mi2 DA. A lower proportion of large river
taxa suggested a loss of biological  integrity  in large
river habitats.  The proportion of large river
individuals in the Lake Agassiz Plain ecoregion
increased with DA (Fig. 9).

-------
 Lake Agassiz Plain
Table 6. Minnow species from the Lake Agassiz
Plain ecoregion of Minnesota and North Dakota
used for evaluating quality pool habitat.
Common name
Scientific name
Central stoneroller
Largescale stoneroller
Spotfm shiner
Carp
Brassy minnow
Common shiner
Silver chub
Flathead chub
Pearl dace
Hornyhead chub
Golden shiner
Pugnose shiner
Emerald shiner
River shiner
Bigmouth shiner
Blackchin shiner
Blacknose shiner
Spottail shiner
Sand shiner
Rosyface shiner
Weed shiner
Mimic shiner
Northern redbelly dace
Finescale dace
Bluntnose minnow
Fathead minnow
Blacknose dace
Longnose dace
Creek chub
Campostoma anomalum
£. oligolepis
Cyprinella spiloptera
Cyprinus carpio
Hybognathus hankinsoni
Luxilus cornutus
Macrhybopsis storeriana
M. gracilis
Margariscus margarita
Nocomis biguttatus
Notemigonus crysoleucas
Notropis anogenus
N. antherinoides
N. blennius
N. dorsalis
N. heterodon
N. heterolepis
N. hudsonius
N. ludibundus
N. rubellus
N. texanus
N. volucellus
Phoxinus eos
P. neogaeus
Pimephales notatus
P. promelas
Rhinichthys atratulus
R. cataractae
Semotilus atromac"latus
                                Table 7.  Minnesota and North Dakota species
                                considered large river taxa following Pflieger
                                (1975) and Simon and Emery (1995).
Common name
Scientific name
Chestnut lamprey
Silver lamprey
Mooneye
Goldeye
Quillback
Bigmouth buffalo
Greater redhorse
Silver chub
Emerald shner
Spottail shiner
Channel catfish
Burbot
White bass
Sauger
Walleye
Freshwater drum
Ichthvomyzon castaneus
I. unicuspis
Hiodon tergisus
H. alosoides
Carpiodes cyprinus
Ictiobus cyprinellus
Moxostoma valenciennesi
Macrhybopsis storeriana
Notropis atherinoides
N. hudsonius
Ictalurus punctatus
Lota lota
Morone chrysops
Stizostedion canadense
S. vitreum
Aplodinotus grunniens

-------
                                  Minnesota-North Dakota Ecoregion
                 .  i  i  •  t
                                  O
                                  O
                                  O
                                  O
                                  O
                                  O
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100
1000
10000      100000
                                  Drainage Area (Sq.  Mi.)
                  Figure 9: Maximum scoring lines for determining trends in proportion of large river individuals with
                  increasing drainage area for the Lake Agassiz Plain ecoregion.

-------
                                                                  Minnesota-North Dakota Ecoreeion
Metric 4. Evenness (All Streams and Rivers)
Impetus

Evenness described the distribution of abundance of
individuals among species by comparing the
observed diversity to a theoretical maximum
(Pielou, 1975). If all species had equal abundance,
the distribution of abundances had maximum
evenness (value = 1).  The greater the differences in
abundance, the smaller the evenness (value
approaches 0).

In many cases where environmental degradation has
occurred, one species in the community will
dominate while the other species decline.  Those
species with the capacity to capitalize on a physical
or chemical change in their environment were
usually tolerant species. In the case of a stream
where degradation had allowed tolerant or exotic
species to increase in abundance to the detriment of
other species, evenness would have decreased as the
proportion of tolerant species increased. Using
evenness as a metric complimented the tolerant
species metric by providing a measure of the degree
that tolerant species dominated a particular
environment.  Thus, reduced evenness indicated a
loss of biotic integrity.

No drainage relationship was observed for this
metric (Fig. 10).  We did not expect any
relationship since this community attribute was not
influenced by stream size categories but disruptions
in community stability.
                                                   25

-------
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                  Figure 10: Maximum scoring lines for determining trends in evenness with increasing drainage area for

                  the Lake Agassiz Plain ecoregion.

-------
                                                                  Minnesota-North Dakota Ecoregion
Metric 5. Number of Sensitive Species (All Streams and Rivers)
Impetus

Karr's (1981) original metric, the number of
intolerant species, included only those species that
were considered highly intolerant to a variety of
disturbances. Karr et al. (1986) further defined
intolerant taxa as those that declined with decreasing
environmental quality and disappeared when the
aquatic environment degraded to fair (Karr et al.
1986). Because very few species at headwater sites
were classified as highly intolerant, Ohio EPA
(1987) modified the intolerant species metric to
include some species classified as moderately
intolerant.  This modified version of Karr's original
intolerant metric was called the sensitive species
metric.

The criteria for determining intolerance for the Lake
Agassiz Plain was based on numerical and graphical
analysis of Ohio's regional data base,  Underbill's
(1989) documentation of historical changes in the
distribution of Minnesota species, and supplemental
information from regional ichthyofaunal  texts
(Pflieger 1975;  Smith 1979; Trautman 1981; Becker
1983; Burr and Warren 1986). The number of
sensitive species distinguished between streams of
highest quality. Designation of too many species as
sensitive prevented this metric from discriminating
among the  highest quality resources. An absence of
sensitive species indicated an anthropogenic stress or
loss of habitat.  Until  improvements in water quality
warrant it,  the sensitive species metric (Ohic EPA
1987) should be retained for all headwater,
moderate sized  streams, and large rivers in the Lake
Agassiz Plain.  Sensitive species increased with DA
among headwater and moderate sized streams and
asymptoted in large rivers (Fig. 11).
Table 8. Fish species found in the Lake Agassiz
Plain of Minnesota and North Dakota that are
considered to be sensitive to environmental
disturbances including water quality and habitat
degradation .
Common name
Goldeye
Mooneye
Hornyhead chub
Pugnose shiner
Blacknose shiner
Blackchin shiner
Sand shiner
Rosyface shiner
Mimic shiner
N. Redbelly dace
Finescale dace
Longnose dace
Northern hogsucker
Silver redhorse
Golden redhorse
Shorthead redhorse
Greater redhorse
Stonecat
Trout-perch
Rock bass
Smallmouth bass
Rainbow darter
Logperch
 Scientific name
 Hiodon alosoides
 H. tergisus
 Nocomis biguttatus
 Notropis anogenus
 N. heterodon
 N. heterolepis
 N. ludibundis
 N. rubellus
 N. volucellus
 Phoxinus eos
 P. neogenus
 Rhinichthys cataractae
 Hypentelium nigricans
 Moxostoma anisurum
 M- erythurum
 M. macrolepidotum
,M- valenciennesi
 Noturus flavus
 Percopsis omiscomaycus
 Ambloplites rupestris
 Micropterus dolomieui
 Etheostoma caeruleum
 Percina caprode
                                                   27

-------
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-------
                                                                 Minnesota-North Dakota Ecoregion
Metric 6. Proportion of Tolerant Individuals (All Streams and Rivers)
Impetus

Karr's (1981) original IBI included the percentage of
the fish community comprised of green sunfish,
Lepomis cyanellus.  The green sunfish was often
present in moderate numbers in many Midwest
streams and became dominant in cases of
degradation or poor water quality. Competitive
advantage in disturbed environments enabled the
green sunfish to survive and reproduce even under
degraded conditions. Although the green sunfish was
widely distributed in the Midwest, it was most
commonly collected in headwater streams. This
introduced bias for moderate sized streams to large
rivers. Karr et al. (1986) suggested  additional
species that could be substituted for  the green
sunfish.  Several species in the Lake Agassiz Plain
were known to increase in abundance along with
increasing degradation of stream quality (Table 9).
By increasing the number of species in this metric
the sensitivity  for different sized streams and rivers
was  improved. This metric detected a decline in
stream quality from fair to poor.

Tolerant species designations were based on Ohio
EPA (1989) and expert consensus between
Minnesota and North Dakota ichthyologists.
Tolerant species were tolerant to thermal loadings,
siltation, habitat degradation, and certain toxins
(Gammon 1983; Ohio EPA 1989).

No DA relationship was evident for small  streams.
However, a negative DA relationship was  observed
in large rivers (> 1500  mi2) (Fig. 12).
Table 9. Fish species found in the Lake Agassiz
Plain ecoregion of Minnesota and North Dakota
that were considered to be highly tolerant to
environmental disturbances including water
quality and habitat degradation.
Common name
Scientific name
Central mudminnow
Carp
Golden shiner
Bluntnose minnow
Fathead minnow
Blacknose dace
Creek chub
Quillback
Bigmouth buffalo
White sucker
Channel catfish
Black bullhead
Freshwater drum
Green sunfish
Brook stickleback
Umbra limi
Cyprinus carpio
Notemigonus crysoleucas
Pimephales notatus
P. promelas
Rhinichthys atratulus
Semotilus atromaculatus
Carpiodes cyprinus
Ictiobus cyprinellus
Catostomus commersoni
Ictalurus punctatus
Amieurus melas
Aplodinotus grunniens
Lepomis cyanellus
Culaea inconstans
                                                  29

-------
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-------
                                                                  Minnesota-North Dakota Ecoregion
Metric 7. Proportion of Omnivore Biomass (All Streams and Rivers)
Impetus

Omnivorous fish species consume significant
quantities of both plant and animal materials
(including detritus) and have the physiological ability
(usually indicated by the presence of a long coiled
gut and dark peritoneum) to utilize both (Goldstein
et al.  1994).  Fishes that do not feed on plants but
on a variety of animal material are not considered
omnivores.

Dominance of omnivores suggested specific
components of the food base were less reliable,
increasing the success of more opportunistic species.
Specialized filter-feeders were not included in this
metric since these species were sensitive to
environmental degradation, e.g. paddlefish,
Polyodon spathula and lamprey ammocoetes,
Lampetra and Ichthyomyzon (Ohio EPA 1989).
Facultative species that shift diet  due to degraded
environmental conditions were also not considered
omnivores, e.g. Semotilus atromaculatus and
Rhinichthys atratulus. The bullheads (Ameiurus spp)
were considered omnivorous.  Although bullheads
did not possess a dark peritoneum and therefore did
not fit the above definition of an omnivore, the
professional opinion of local biologists was that the
bullheads function as omnivorous members of the
fish community in the Lake Agassiz Plain ecoregion.
This metric evaluated the intermediate to low
categories of environmental quality (Table 10).

We chose to use the proportion of omnivore biomass
rather than percentage of individuals  since biomass
more appropriately reflected the utilization of energy
within the fish community. These values differed
from the omnivore percentages of Karr et al. (1986)
that was based on the number of  individuals,  but
resemble Ohio EPA's (1987) classification. No
relationship with DA was found for headwater,
moderate streams, or large river  sites (Fig. 13).
Table 10. List of Minnesota and North Dakota
fish species considered to be omnivores.
Common name
Scientific name
Carp

Pugnose shiner

Bluntnose minnow

Fathead minnow

Quillback

White sucker

Bigmouth buffalo

Brown bullhead

Yellow bullhead

Black bullhead
Cyprinus carpio

Notropis anogenus

Pimephales notatus

P. promelas

Carpiodes cyprinus

Catostomus commersoni

I. cyprinellus

Ameiurus nebulosus

A. natalis

A,, melas
                                                   31

-------
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10000
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                  Figure 13: Maximum scoring lines for determining trends in proportion of omnivore biomass with
                  increasing drainage area for the Lake Agassiz Plain ecoregion.

-------
                                                                 Minnesota-North Dakota Ecoreeion
Metric 8. Proportion of Insectivore Biomass (All Streams and Rivers)
Impetus

The original IBI included the proportion of
insectivorous cyprinidae (Karr et al.  1986).
Insectivorous species are an important link in
transferring energy between lower trophic levels to
keystone predator species. This metric was intended
to respond to a depletion of the benthic
macroinvertebrate community that comprised the
primary food base for most insectivorous fishes. As
disturbance increased, the diversity of insect larvae
decreased, triggering an increase in the omnivorous
trophic level. Thus,  this metric varied inversely with
the omnivore metric with increased environmental
degradation. The inclusion of all insectivorous
species (Table 11) was based on the observation that
not all regions of Minnesota and North Dakota
possess high proportions of insectivorous cyprinids
in high quality streams.  There was no DA
relationship with the proportion of insectivorous
fishes in the Lake Agassiz Plain ecoregion (Fig. 14).
Table 11. Minnesota and North Dakota
species considered to be insectivores.
                                Table 11. (Continued)
                                Common name
                        Scientific name
Common name
Scientific name
Goldeye
Mooneye
Silver redhorse
Golden redhorse
Shorthead redhorse
Greater redhorse
Northern hogsucker
Longnose dace
Blacknose dace
Northern redbelly dace
Finescale dace
Hiodon alosoides
iL tergisus
Moxostoma anisurum
M. erythrurum
M. macrolepidotum
M. valenciennesi
Hypentelium nigricans
Rhinichthys cataractae
R. atratulus
Phoxinus eos
P. neogaeus
 Pearl dace
 Flathead chub
 Silver chub
 Horneyhead chub
 Creek chub
 Golden shiner
 Spottail shiner
 Bigmouth shiner
 River shiner
 Rosyface shiner
 Blackchin shiner
 Blacknose shiner
 Weed shiner
 Sand shiner
 Mimic shiner
 Emerald shiner
 Common shiner
 Spotfin shiner
 Stonecat
 Tadpole madtom
 Central mudminnow
 Freshwater drum
 Yellow perch
 Blackside darter
 Logperch
 Iowa darter
 Rainbow darter
 Least darter
Johnny darter
Green sunfish
Bluegill
Orangespotted sunfish
Pumpkinseed
Brook stickleback
Trout-perch
 Margaricus margarita
 Macrhvbopsis gracilis
 M. storeriana
 Nocomis biguttatus
 Semotilus atromaculatus
 Notemigonus crysoleucas
 Notropis hudsonius

 £L blennius
 N. rubellus
 N. heterodon
 N. heterolepis
 N. texanus
 N. ludibundus
 N^ volucellus
 £L atherinoides
 Luxilus cornutus
 Cyprinella spiloptera
 Noturus flavus
 N. gyrinus
 Umbra limi
 Aplodinotus grunniens
 Perca flavescens
 Percina maculata
 P. caprodes
 Etheostoma exile
 3L caeruleum
 E. microperca
 E, nigrum
 Lepomis cyanellus
 L. macrochirus
 L^ humilus
L. gibbosus
Culaea inconstans
Percopsis omiscomavcus

-------


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Figure 14: Maximum scoring lines for determining trends in proportion of insectivore biomass

with increasing drainage area for the Lake Agassiz Plain ecoregion.

-------
                                                                 Minnesota-North Dakota Ecoregion
Metric 9. Proportion of Pioneer Species (Headwater Streams)
         Proportion of Piscivore Biomass (Moderate Sized Streams and Large Rivers)
Impetus

Karr (1981) developed the carnivore metric to
measure community integrity in the upper trophic
levels of the fish community.  It is only in high
quality environments that species occupying the
upper trophic  levels were able to flourish.
Proportion of Pioneer Species

Piscivores were generally not abundant in headwater
streams. An alternate metric was developed by Ohio
EPA (1987) to determine the permanence of the
stream habitat. Smith (1971) identified a signature
assemblage of small stream species that he called
"pioneer species" (Table 12). These species were
the first to colonize sections of headwater streams
after desiccation and also tended to predominate in
unstable environments affected by anthropogenic
stresses. Thus, a high proportion of pioneer species
indicated an environment that was unstable or
stressed. The  metric was negatively associated with
increases in DA (Fig.  15).

Proportion of Piscivore Biomass

This metric included species in which the adults are
predominantly piscivores, although some may feed
on other vertebrates or invertebrates such as crayfish
(Karr et al. 1986). Species that are opportunistic do
not fit into this metric, e.g. creek chub (Karr et al.
1986; Ohio EPA 1987).

Karr (1981) suggested that the proportion of
piscivores should be related to DA. Such a
correlation was not found in the  Lake  Agassiz Plain
ecoregion (Fig. 16). Piscivore species found in the
Lake Agassiz  Plain ecoregion were listed  in Table
13. Because most piscivores found in this region
were managed for sportfishing, the natural balance
or proportion  of piscivores sometimes outweighed
other species.   Therefore, the metric has an upper
limit of 30%  piscivore biomass.
Table 12. Pioneer species of the Lake Agassiz
Plain ecoregion that are considered to be
indicators of temporally unavailable or desiccated
habitats (Larimore and Smith 1963; Smith 1971).
Common name
Scientific name
Central stoneroller
Largescale stoneroller
Bluntnose minnow
Fathead minnow
Creek chub
Green sunfish
Johnny darter
Campostoma anomalum
C. oligolepis
Pimephales notatus
P. promelas
Semotilus atromaculatus
Lepomis cyanellus
Etheostoma nigrum
Table 13. Fish species of the Lake Agassiz Plain
ecoregion that are piscivores.
Common name
Scientific name
Silver lamprey
Chestnut lamprey
Northern pike
Muskellunge
White bass
Rock bass
Smallmouth bass
Largemouth bass
Walleye
Sauger
Burbot
Channel catfish
Bow fin
Black crappie
White crappie
Ichthvomyzon unicuspis
I. castaneus
Esox lucius
 E. masquinongy
Morone chrysops
Ambloplites rupestris
Micropterus dolomieui
M. salmoides
Stizostedion vitreum
S. canadense
Lota lota
Ictalurus punctatus
Amia calva
Pomoxis nigromaculatus
£L annularis

-------
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 Lake Agassiz Plain
Metric 10. Number of Individuals per Meter (All Streams and Rivers)
Impetus
This metric evaluated population density and was
expressed as catch-per-unit of effort. Effort may be
defined as the length of stream reach sampled, unit
of area sampled, or unit time spent sampling.  Low
catch-per-unit effort values indicated that biotic
integrity was being  compromised and that the
normal trophic relationship of fish communities was
being altered.  Although biotic integrity was
generally considered to be positively correlated with
catch per unit effort, numerous exceptions existed.
For instance, as biotic integrity increased, the catch-
per-unit effort often became more variable because
numerous physical and chemical characteristics of
the stream began to limit species abundance.  Also,
under certain circumstances (e.g. channelization)
reduced canopy cover increased stream productivity
by allowing light to penetrate to the stream surface.
This may have lead to an increase in the abundance
of certain tolerant fishes (Ohio EPA 1987). Lyons
(1992) found that abundance, excluding tolerant
species, was greatest at fair quality sites in
Wisconsin warmwater streams and lower at sites
classified as excellent. Karr et al. (1986) suggested
that this metric was most sensitive at intermediate to
low ends of the sensitivity continuum.

For the Lake Agassiz Plain ecoregion, catch-per-
unit effort was calculated as the total number of
individuals collected per meter of stream read,
sampled. (See materials and methods,  community
analysis, sample considerations).  A DA-dependent
relationship was observed for the Lake Agassiz Plain
ecoregion (Fig. 17).
                                                    38

-------
L: n

-------
 Lake Agassiz Plain
Metric 11.  Proportion of Individuals as Simple Lithophilic Spawners (All Streams)
Impetus

Ohio EPA (1987) replaced the original metric,
proportion of hybrids (Karr et al. 1986), with this
metric. The original intent of the hybrid metric was
to assess the extent to which degradation had altered
reproductive isolation among species. However,
difficulties of identification,  lack of occurrence in
headwater and impacted streams, and presence  of
hybrids  in high quality streams,  (e.g., cyprinids and
centrarchids) caused this metric  to be rather
insensitive.

The spawning guild concept  has been suggested to
be an important metric (Angermeier and Karr 1986).
The success of certain spawning guilds may be
affected by habitat quality (Balon 1975;  Berkman
and Rabeni 1987).  Simple spawning behavior  (i.e.
lithophilous) requires clean gravel or cobble for
success  and may be the most environmentally
sensitive (Ohio EPA 1987).  Simple  lithophils (Table
14) broadcast eggs that come into contact with the
substrate. Eggs develop in the interstitial spaces
between sand, gravel, and cobble without parental
care.  Simple lithophils that do not require clean
substrates for reproduction were not included in this
metric.  These species have rapidly developing  eggs
and larvae that are buoyant or phototaxic and require
minimal contact with the substrate.
Table 14. Species in the lake Agassiz Plain
ecoregion that are considered to be simple
lithophilic spawners.
Common name
Scientific name
River shiner
Rosyface shiner
Northern redbelly dace
Blacknose dace
Longnose dace
White sucker
Northern hogsucker
Silver redhorse
Golden redhorse
Shorthead redhorse
Greater redhorse
Burbot
Rainbow darter
Logperch
Blackside darter
Sauger
Walleye
N. blennius
N. rubellus
Phoxinus eos
Rhinichthys atratulus
R. cataractae
Catostomus commersoni
Hypentilium nigricans
Moxostoma anisurum
M- ervthrurum
M. macrolepidotum
M. valenciennesi
Lota lota
Etheostoma caeruleum

P. maculata
Stizostedion canadense
S. vitreum
Simple lithophils were sensitive to environmental
disturbance, particularly siltation.  Berkman and
Rabeni (1987) observed a negative relationship
between simple lithophilic spawners and the
proportion of silt in streams. Historically, some
simple lithophilic spawners have experienced
significant range reductions due to increased silt
loads in streams. The proportion of lithophilic
species was not related to DA (Fig.  18).
                                                   40

-------
                                                  Minnesota-North Dakota Ecoregion










































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Metric 12. Proportion of Individuals with Deformities, Eroded Fins, Lesions and Tumors (DELT)
           (All Streams)
Impetus

This metric evaluated the condition of fish based on
the percentage of external anomalies such as
deformities, eroded fins, lesions, and tumors
(DELT). The proportion of individuals with DELT
anomalies corresponded to the percentage of
diseased fish in Karr's (1981) original index.  Studies
of fish populations indicated that these anomalies
were either absent or occurred at very low rates
naturally, but reached higher percentages at
impacted sites (Mills et al. 1966; Berra and Au
1981; Baumann et al. 1987). Common causes for
DELT anomalies were bacterial, fungal,  viral, and
parasitic infections, neoplastic diseases,  and
chemicals (Allison et al. 1977;  Post 1983; Ohio
EPA 1987). An increase in the frequency of
occurrence of these anomalies was an indication of
physiological stress due to environmental
degradation, chemical pollutants, overcrowding,
improper diet, excessive siltation, and other
perturbations. In Ohio, the highest incidence  of
deformities, eroded fins,  lesions, and tumors
occurred in fish communities downstream from
dischargers of industrial and municipal wastewater
and areas subjected to intermittent stresses from
combined sewers and urban runoff. Leonard and
Orth (1986) found that this metric corresponded to
increased degradation in streams in West Virginia.
Karr et al. (1986)  observed that this metric was most
sensitive in low quality streams.

The presence  of the parasite black spot is not
 included in the DELT metric because infestation
 varied in degree and was a function of the presence
 of snails, an intermediate host.  Thus, it was  not
 solely related to environmental degradation (Allison
 et al. 1977; Berra and Au 1981). Whittier et  al.
 (1987) and Steedman (1988) showed no  relationship
 between stream quality and black spot infestation.
 Other parasites were also excluded due  to the lack of
 a consistent relationship with environmental
 degradation.
Ohio EPA (1987) modified scores when individuals
numbered less than 200, and DELT fish numbered
over two fish.  No scoring modifications were
necessary for sites in the Lake Agassiz Plain since
fish with DELT anomalies were uncommon and low
numbers of species did not affect the performance of
this metric (see low score modifications). This
metric may be more important in the future as
industrial development in the basin becomes more
widespread. The proportion of individuals with
DELT anomalies was not correlated with DA.

-------
                       Minnesota-North Dakota Ecoregion
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-------
 Lake Agassiz Plain
Alternative Metrics. Proportion of Subterminal-Mouth Minnows (Moderate Sized Streams)
                    Number of Sucker Species (Moderate Sized Streams and Large Rivers)
Impetus

Four alternative metrics were considered for
inclusion into the Lake Agassiz Plain IBI, but were
not included in the scoring. The alternative metrics
were used at the discretion of the  researcher in
situations where the final IBI score was close to the
integrity classification cuttoffs or  where  a particular
metric did not seem to be performing well.

The proportion of headwater individuals metric and
the proportion of pioneer individuals metric were
applicable in moderate sized streams as well as
headwater streams (Tables 3 and 4; Fig. 5 and 15).
The rationale for including these metrics in  the Lake
Agassiz Plain IBI was described earlier.

The proportion of subterminal-mouth minnows and
the number of sucker species metrics were not
included in the scoring for any stream size
classification (Table 15 and 16; Fig. 20 and 21).
Because subterminal mouth minnows and suckers
were primarily benthic insectivores, they depended
on the availability of interstitial habitat to provide
food and cover.  A high proportion of subterminal
mouth minnows and sucker species were associated
with high biotic integrity and a lack of siltation.
Table 15. Subterminal-mouth minnow species in
the Lake Agassiz Plain ecoregion.
Common name
Scientific name
Central stoneroller
Largescale stoneroller
Longnose dace
Silver chub
Bluntnose minnow
Bigmouth shiner
River shiner
Sand shiner
Campostoma anomalum
C. oligolepis
R. cataractae
Macrhybopsis storeriana
Pimephales notatus
Notropis dorsalis
N. blennius
N. stramineus
Table 16. Sucker species in the Lake Agassiz
Plain ecoregion.
Common name
Quillback
White sucker
Northern hogsucker
Bigmouth buffalo
Silver redhorse
Golden redhorse
Shorthead redhorse
Greater redhorse
Scientific name
Carpiodes cyprinus
Catostomus commersoni
Hypentelium nigricans
Ictiobus cyprinellus
Moxostoma anisurum
M- erythrurum
M. macrolepidotum
M- valenciennesi
                                                   44

-------
                                         Minnesota-North Dakota Ecoregion
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      Figure 21: Maximum scoring lines for determining trends in number of sucker species with increasing
      drainage area for the Lake Agassiz Plain ecoregion.

-------
                                                                  Minnesota-North Dakota Ecoregion
Scoring Modifications

Samples with only a few species or extremely low
numbers in the catch presented a scoring problem
for some of the proportional metrics. Scoring
adjustments were made to reduce the possibility of
giving high scores to degraded sites. Aquatic
habitats impacted by anthropogenic disturbances
exhibited a disruption in the food base and the
sample consisted of very few species or numbers of
individuals. At such low population levels the
normal  structure of the community was
unpredictable (Ohio EPA 1987).  Ohio EPA (1987)
observed that the proportion of omnivores,
insectivorous fishes, and percent individuals affected
by anomalies did not always match expected trends
when fewer than  100 individuals were collected.
Although scores were expected to deviate strongly
from those of high quality areas, this was not always
observed.

Similar to Ohio EPA (1987), Bailey et al. (1993)
found that in the Minnesota River Basin many of the
proportional metrics were unpredictable when the
number of species at a site were extremely low. At
these sites some of the proportional metrics did not
reflect the quality of the resource because percent
composition was unpredictably influenced by the
presence of a few species.  Low score modifications
were necessary for the proportional metrics when
the "number of species" metric scored a "1"
indicating severe impairment.

Unlike  Ohio however, the percentage of DELT
anomalies metric was  not modified in the Minnesota
River basin because very few sites had species that
exhibited these anomalies.  This is probably because
most sites were not directly impacted by industrial
pollutants. Rather, habitat destruction through
channelization and sedimentation were the primary
anthropogenic influences on stream quality. Similar
to the Minnesota River basin, few DELT anomalies
were found in fish from the Lake Agassiz Plain
ecoregion.

The following scoring modifications, based on
information from Bailey et  al. (1993) and Ohio EPA
(1987), were adopted for evaluating sites in the Lake
Agassiz Plain ecoregion.  Modifications were applied
where scoring became unpredictable due to a low
number of individuals or species.

Headwater streams:  Proportion of omnivore
insectivore, simple lithophil, and pioneer species
metrics should be scored a " 1" if < 25 individuals
were collected at a site or the number of species
metric is scored a " 1".

Moderate sized streams and Large Rivers:  The
proportion of piscivore, omnivore, insectivore, and
simple lithophil metrics should be scored a " 1" if <
50 individuals were collected at a site or when the
number of species metric is scored a "1".

No scoring adjustments were necessary for
proportion of tolerant species or the percent DELT
anomalies. Further evaluation  is needed to
determine if scoring modifications is necessary for
the other proportional metrics.  In all cases, the
biologist's best professional judgement should be
used to decide when low-score  modifications  are
appropriate.
                                                   47

-------
 Lake Agassiz Plain
4.0 RESULTS AND DISCUSSION

Lake Agassiz Plain

Species Composition: One hundred and eleven sites
were sampled in the Lake Agassiz Plain during 1993
and 1994 (Fig. 3). Sixty three species were
collected in 16 families (appendix A). This
represents about 73 % of the 86 species reported
(Koel and Peterka 1994) to occur in the Red River
basin. Numerically, cyprinids dominated the catch
(74%) followed by catostomids (9%). The most
abundant species were fathead minnow Pimephales
promelas (20%), common shiner Luxilus cornutus
(17%), and creek chub Semotilus atromaculatus
(11%).

Cyprinids also dominated the catch in terms of
biomass (40%). However, the common carp
Cyprinus carpio made up 64% of the cyprinid
biomass.  If we exclude the common carp from the
catch, catostomids were the dominate family by
weight (35%) followed by cyprinids (14%).  The
dominant species by weight was the common carp
(25%) followed by white sucker Catostomus
commersoni (9%), and channel catfish Ictalurus
punctatus (8%).

Three species: the rainbow trout Oncorhvnchus
mykiss. brook trout Salvalinus fontinalis. and white
bass Morone chrysops were stocked for sport
fisheries. The common carp was the only non-
gamefish introduction collected during the study.

Trends in IBI Scoring: The IBI scores were highly
variable throughout the Lake Agassiz Plain.  There
was virtually no correlation between IBI scores and
DA (r2=0.0838) (Fig. 22). IBI scores ranged from
16 to 48 or  from very poor to good using Karr's
integrity classification system modified for the  Lake
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          • = North Dakota Sites
          • = Red River
                 ir2 =0.0838
                       10             100             1000

                                  Drainage area (miles2)
                   10000
                                                                                         100000
      Figure 22: Plot of drainage area vs. IBI score for all sites sampled during the study period, 1993-1994.
      triangle markers represent sites sampled in Minnesota., circle markers for North Dakota, and square markers
      for sites sampled on the Red River.
                                                 48

-------
                                                             Minnesota-North Dakota Ecoregion
   o>
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                                                                       DAM  Sites
                                                                          Minnesota
                                                                       l~lMr.rth Dakota
30
         25
         20
              Headwater
              Streams
                        Moderate
                        Streams
Large
Rivers
All  Size
Classifications
         Figure  23:  Mean ffil s cores  fo r head w ate r, mode rate , large rivers and
         all s ize s tream s . S co res for Minnes ota and North Dakota excluded
         the Red Riversites  while  the  scores  forallsites included the Red
         Rive r s ites .  As te ris  ks  indie  ate d a s ig nific ant d iffe re nc e  from
         otherstream  sizes  (ANOVA,  P<0.05).
Agassiz Plain ecoregion (Table 1). The average
overall score was 32 (Fig. 23). Forty percent of all
sites were rated fair (Fig. 24). There were no
excellent sites (>50)  in the ecoregion (Fig. 24).

Although we attempted to find the highest quality
sites for the purpose of establishing reference
conditions, the sites selected for this study  should
not be considered pristine.  The sites that we
selected represented the best conditions present in
the basin but most likely did not reflect historical
environmental conditions for this  ecoregion.  The
rivers and streams in  the Lake Agassiz Plain
ecoregion were modified extensively through
channel modification, etc. With improved land and
water  management practices we expect some sites to
move  into the excellent category (> 50).

Metric score averages provided insight into fish
community condition  in the ecoregion.  For
                                           instance, the average metric score for the percentage
                                           of DELT anomalies was very high (4.56) indicating
                                           that pollution sources within the ecoregion were not
                                           affecting fish health. Increases in the prevalence of
                                           DELT anomalies have been found in degraded
                                           stream habitats, often associated with industrial and
                                           municipal discharges (Simon 1992).  Since we
                                           attempted to avoid these areas  we did not expect to
                                           see significant numbers of DELT anomalies. The
                                           proportion of omnivore biomass score averaged 3.30
                                           and the proportion of insectivore biomass averaged
                                           2.98.  These scores  were high  relative to the other
                                           metric scores. This indicates that streams within the
                                           ecoregion generally  had a stable food base that
                                           allowed the more specialized feeders to thrive. The
                                           proportion of simple lithophilic spawners scored the
                                           lowest of all metrics. Siltation was the most likely
                                           limiting factor affecting those species that relied on
                                           clean gravel and cobble substrates to successfully
                                           reproduce.
                                               49

-------
 Lake Agassiz Plain
Headwater, Moderate, and Large Rivers

Species Composition: Community composition
varied among headwater streams, moderate sized
streams, and large rivers.  Sampling at headwater
streams (< 200 mi2 DA) found 33 species in 9
families.  The most abundant species in headwater
streams were fathead minnows (41%), blacknose
dace Rhinichthys atratulus (12%), and brook
stickleback Culaea inconstans (11%).  Forty seven
species in 14 families were found in moderate sized
streams  (200-1500 mi2 DA).  The most abundant
species in moderate sized streams were common
shiners (23%), creek chubs (13%), and fathead
minnows (13%). Sampling in large rivers ( > 1500
mi2 DA) found 55 species  in 14 families.  The
dominant species in large rivers were fathead
minnows (16%), common shiners (14%), and
spotfin shiners Cyprinella  spiloptera (11%).

Trends in IBI Scoring: The average IBI score for
                                           headwater streams was 29.1,  which was
                                           significantly lower (ANOVA, p< .05) than either
                                           moderate streams (mean = 34.1) or large rivers
                                           (mean=35.3) (Fig. 23).  Fifty five percent of all
                                           headwater streams were rated poor or very poor.
                                           This contrasted with the better rating  of moderate
                                           sized streams  and large rivers. Only  28% of
                                           moderate sized streams were  rated poor or very
                                           poor and only 12% of large rivers were rated as
                                           such (Fig. 24).

                                           There were a number of plausible explanations for
                                           the low biotic integrity of headwater streams in the
                                           Lake Agassiz  Plain ecoregion. Stream
                                           channelization, parricularily prevalent in headwater
                                           stream reaches, had undoubtedly led to some
                                           impairments to resource quality.  Its negative effects
                                           were most pronounced on small headwater streams
                                           where many have been straightened and rerouted to
                                           serve as field drainage systems for row crops.
                                           Stream channelization  reduced habitat quality by
                                           reducing pool  depth and substrate heterogeneity,
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                                                                  Minnesota-North Dakota Ecoregion
altering riffle-run-pool sequences, increasing
turbidity, and reducing the retention time for water
remaining in the stream channel (Rankin et al.
1992).

Due to the degree of channelization in this
ecoregion, it was impossible to totally avoid the
negative influence of channelization on habitat
quality. Many headwater sites, although  not
channelized, were often surrounded upstream and
downstream by large segments of channelized
stream. These unaltered  stream segments, while
certainly not pristine, represented the best conditions
found in many of these streams and provided an
important habitat refuge for biota.

In addition to channel modification, headwater  sites
often underwent extreme  fluctuations in flow and
created temporal changes in habitat availability for
fish.  Low flow conditions at headwater  sites
benefited pioneer spieces by forcing fish to move out
of an  area for a period of time and then recolonize
the area later when conditions improved.
Conversely, high flow conditions also created
unstable environments due primarily to increased
velocity and turbidity. These conditions were
exacerbated by channelization that was so prevalent
in the Lake Agassiz Plain ecoregion.  As expected, a
relatively large proportion (39%) of the total number
of species collected in headwater environments were
pioneer species. These species were  opportunists
that were able to take advantage of temporarily
available habitat.

The importance of headwater streams cannot be over
emphasized.  Although headwater streams in this
ecoregion did not typically support year-round game
fish populations, they did serve an important
function in providing many game fish species with
suitable spawning habitat. In addition, headwater
streams were important components in river
drainage networks and served as an entry point for
pollutants from the terrestial environment (Bailey et
al.  1993). Therefore, the condition of moderate
sized  streams and large rivers was intimately tied to
the condition of headwater streams.
Minnesota and North Dakota

Species Composition:  In general, streams in the
eastern portion of the basin had a higher species
richness than those in the west.  Excluding the
border waters of the  Red River, 52 species were
collected in Minnesota streams while 46 species
were collected in North Dakota.

Some of the rivers in the basin, like the Otter Tail
River of Minnesota,  were known to have a high
species diversity (Koel and  Peterka 1994). We
collected 23 species from one site on the Otter Tail
River that included 4 species not collected in any
other river during the study: green sunfish Lepomis
cyanellus. largemouth bass  Micropterus salmoides.
greater redhorse Moxostoma valenciennesi. and
logperch Percina caprodes.  The Otter Tail was like
many of the other tributaries to the Red River in
Minnesota in that it flowed  through a number of
ecoregions.  Originating in  the Northern Lakes and
Forests ecoregion,  the Ottertail flowed southward
into the Central Hardwood  Forests ecoregion before
turning west into the  Lake Agassiz Plain. The
rolling hills and abundance  of lakes and wetlands in
the upper two ecoregions contrasted sharply with the
relatively featureless  topography of the Lake
Agassiz Plain ecoregion. This diversity of habitat,
while common to tributaries in the eastern portion  of
the basin, was not present in the western side of the
basin.

A number of species  were unique to one state, either
Minnesota or North Dakota. Species collected only
in Minnesota included mooneye Hiodon tergisus.
goldeye Hiodon alosoides. bigmouth buffalo Ictiobus
cyprinellus. silver lamprey  Ichthyomyzon unicuspis.
green sunfish, pumpkinseed Lepomis gibbosus.
burbot Lota lota, largemouth bass, greater redhorse,
blacknose shiner Notropis heterolepis. logperch,
finescale dace Phoxinus neogeaus. sauger
Stizostedion canadense. and central mudminnow
Umbra limi.  Eight of these species were collected
from either the Otter Tail or Wild Rice Rivers of
Minnesota. The central mudminnow was found
extensively throughout  the Minnesota portion of the
basin (33 sites) but was not  collected from North
Dakota during the study.  Eight species were
collected only in North Dakota: largescale
                                                   51

-------
 Lake Agassiz Plain
stoneroller Campostoma oligolepis. banded killifish
Fundulus diaphanus. brown bullhead Ameiurus
nebulosus. brassy minnow Hybognathus hankinsoni.
bluegill Lepomis macrochirus. orangespotted sunfish
Lepomis humilis. golden shiner Notemigonus
crysoleucas. and rainbow trout. Six of the 8 species
collected exclusively from North Dakota were from
either the  Turtle or Sheyenne Rivers.

Trends in IBI Scoring:  IBI scores were slightly
higher for streams in Minnesota compared to those
in North Dakota (Fig.  23).   Although significant
differences in IBI scoring were not found between
the states, there were some differences in individual
metrics.  For instance,  the species richness metric
scored higher at Minnesota streams for all stream
size classifications.    These differences in species
richness were expected for reasons given earlier.
Somewhat more perplexing is that most of the
biomass metrics and the catch-per-unit effort metric
scored higher in North Dakota than Minnesota. Only
insectivore biomass in headwater streams was higher
in Minnesota. The higher catch rates in North
Dakota suggested that these streams may be more
productive.

Pioneer species occurred much more frequently in
North Dakota headwater streams. With the
exception of the green sunfish, every pioneer species
was more common in  North Dakota.  The likely
explanation for this may lie in the temporary nature
of headwater streams in North Dakota that  was
related to climatic and topographical factors.
Headwater streams in North Dakota typically
originated in relatively dry prairie environments.
Because of this, periods of drought may have caused
rapid and prolonged decreases in stream flow
making some sections of the stream uninhabitable to
fish.  In contrast, Minnesota headwater streams
were linked with wetland habitat that  had the effect
of modifying these hydrologic extremes. In
addition,  the Minnesota portion of the Red  River
basin received more annual precipitation than North
Dakota.

Sensitive species were much more common in
Minnesota. The average IBI score for this metric in
 Minnesota streams was 2.51.  This is  almost a full
point higher than the average IBI score of  for
streams in North Dakota.  There were 7 sensitive
species collected in Minnesota that were not found
in North Dakota.  These species included the
goldeye, mooneye, greater redhorse, blacknose
shiner, log perch, irout perch Percopsis
omiscomaycus. and finescale dace. Sensitive species
such as the northern hogsucker Hypentelium
nigricans. rainbow darter Etheostoma caeruleum.
pugnose Notropis anogenus. mimic Notropis
volucellus. and blackchin shiners Notropis heterodon
were known to historically occur in the ecoregion,
but were not collected in either state during the
study period.

The Red River

Species  Composition:  The Red River was sampled
at 8 locations from sites close to its source to sites
near the Canadian border (Fig. 25). Thirty six
species in 12 families were collected from the Red
River.  Spotfin shiner (38%),  emerald shiner
Notropis atherinoides  (33%), and silver  chubs
Macrhybopsis storeriana (4%) were the most
abundant species.  Shorthead redhorse Moxostoma
macrolepidotum (20%), silver redhorse Moxostoma
anisurum (16%), and common carp (15%)
dominated the catch in terms of biomass.  Three
species: the white bass, silver chub, and  river shiner
Notropis blennius were unique to the Red River.
This survey compared favorably with the  1983-1984
Red River survey by Renard et al. (1986). Renard et
al. (1986) sampled 40 stations and collected the
same number of species (36).  This indicated that
the 8 sites sampled in this study were sufficient to
characterize the fish community of me Red River.

Trends in IBI Scoring: Sites surveyed in the Red
River ranged from fair (score of 32) to good (score of
48) based on IBI scoring criteria (Fig. 25). IBI
scores were generally higher in the Red River
compared to other large rivers in the ecoregion. The
mean IBI score for the Red River was 39.5 while
other large river sites averaged 33.4.  Metrics that
scored particularily well in the Red River included
the number of large river species, proportion of
omnivore species, and proportion of tolerant
individuals.  The tolerant species metric attained a
maximum score of 5 at every site sampled. The
number of simple lithophilic individuals and

-------
                                                                  Minnesota-North Dakota Ecoregion
proportion of round-bodied suckers metrics scored
poorly in the Red River (mean scores of both were
1.5). This suggested that many fish species were
doing quite well in the Red River but siltation may
be limiting those fish species that required clean
gravel substrates to successfully reproduce.

The 8 sites located on the Red River were widely
dispersed and provided insight into the biotic
integrity of this river along a longitudinal gradient
(Fig. 25).  In spite of the input from a multitude of
potential pollution sources, IBI scores did not
decrease with increasing distance downstream.
Rather, some of the highest scoring sites were located
nearest the Canadian border.

The evenness and the CPUE metrics scored
particularity well at the downstream sites indicating
an abundant, well-balanced fish population that was
not dominated  by opportunistic or tolerant species.
However, simple lithophils and round-bodied sucker
species metrics continued to score poorly at the
downstream sites.
  Community Trends in the Red River
bO
40
§ 30
> 20
a 10
0
» • •
L *




      550     450     350     250
          River Miles From Mouth
150
Figure 25: Longitudinal community trends in IBI
scores for the Red River. The circle marker (river
mile 173) is an average.

Variability

Although variability throughout the Lake Agassiz
Plain was quite evident, variability in sampling
technique between agencies needed to be eliminated
as a source  Two types of variability were examined
to determine if any bias in sampling occurred.
Temporal variability was measured at eight sites.
These sites were sampled during different years
(1993-1994) and sampled the same year during
different time periods. Sampling procedures
remained the same between time periods; however,
many of the sites were repeated with different
crews.  Differences between years were not
significantly different (ANOVA, p>0.05).
Differences for sites repeated during the same year
were also not significantly different (ANOVA,
p>0.05). Coefficient of variation (CV), was used to
compare the relative amounts of variation at each
site.  Mean CV for all eight sites and all 20 samples
was 9.06 percent.

Five sites,  each with three consecutive reaches,
were sampled on the same day by a single crew  to
examine the adaquacy of the reach length. The
mean CV for these sites was 7.3 percent. Less than
two percent difference existed between temporal
variability with multiple samplers  and the reach
variability.

This data suggested  that reach lengths were
sufficient to characterize the fish community and
that bias among samplers,  sampling time or
sampling equipment was not significant.

Reference Sites

A list of reference sites was developed from data
collected during 1993-1994 for the Lake Agassiz
Plain ecoregion (Table 17).  Inclusion of a site into
this list was based on superior IBI scores, habitat
characteristics,  and professional judgement. Since
these sites were thought to represent the reference
condition, future monitoring  efforts should
concentrate on these sites to recalibrate the metrics
and further refine the reference  site list.  Hughes
(1995) suggested that the reference condition should
be set as high as possible to adequately protect the
resource. Since the reference condition in the Lake
Agassiz Plain represented a considerable amount of
disturbance, only sites with IBI scores in the 90th
percentile were considered reference sites.

As  mentioned earlier,  these sites should not be
considered pristine but may well be described as the
"least impacted" sites  for the Lake  Agassiz Plain.  It

-------
 Lake Agassiz Plain
was also important to consider that reference
conditions do not remain static. On the contrary,
repeat monitoring and sampling of stations, both
reference and site specific, will be needed in order
to add to and refine the list of reference sites,
document change over time, and further refine the
IBI for the Lake Agassiz Plain ecoregion.
                                                    54

-------
                                                              Minnesota-North Dakota Ecoregion
Table 17: Reference sites determined using fish community biotic integrity for the Lake Agassiz Plain
ecoregion, Minnesota, North Dakota, and the Red River.
Minnesota
North Dakota
Red River
South Branch Wild Rice River:  E of CSAH 27 bridge, T 142 R 45 S 16. Long:
96° 24' 08" Lat: 47° 07' 01" (site: 94-012)

South Branch Wild Rice River:  W of CR 136 bridge, T 143 R 46 S 30.
Long: 96° 33'35" Lat:  47° 10' 15" (site: 94-013)

Buffalo River:  USGS site near Buffalo River State Park, Field # 46514909, T 139
R46S11. Long: 96° 28'04"  Lat: 46° 51'49" (site: 94-507)

Buffalo River: At CR26, 2.5 mi E of Kragnes, T 141 R48S36.  Long: 96° 41'
26" Lat:  46° 58' 39" (site: 94-019)

Marsh River: North of CR  129 bridge, T 146 R 48 S 34.  Long 96° 45' 58"  Lat:
47° 24'44" (site: 94-014)

North Branch Turtle River: SH  18 bridge, 3 mi N of Hwy 2 Junct.
Long: 97° 37' 25" Lat: 47° 59' 31" (site:94-125)

Turtle River: At State Park. Long: 97° 30' 00" Lat: 47° 56' 18".  (site: 94-502, 94-
523 94-524)

Forest River: 2 mi N Inkster. Long: 97° 38' 37" Lat: 48°  10' 46". (site: 94-130)

Goose River:  USGS site at Hillsboro, Field # 0506650. Long: 97° 03' 39" Lat: 47°
24' 34" (site: 94-504)

Pembina River: USGS site at Walhalla, Field # 05099600. Long: 97° 55'00"  Lat:
48° 54' 50" (site: 94-505)

Red River: East of Joliette. Long: 97° 10' 43" Lat: 48° 48' 51". (site: 94-528, 94-
529, 94-530)

Red River: 0.5 mi W of Hwy 75, T 133 R 47 S 21. Long: 96° 36'03" Lat: 46°
19'36" (site: 94-021)

Red River: 0.5 mi W of end of CR 109, T 144 R 48 S 1.  Long: 96° 49' 45" Lat:
47° 19' 35" (site: 94-018)
                                               55

-------
 Lake Agassiz Plain
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                                                                 Minnesota-North Dakota Ecoregion
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 Lake Agassiz Plain
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                                                  58

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                                                               Minnesota-North Dakota Ecoregion
6.0 APPENDICES

 A. Current and historical fish species found in the Red River Basin (native, exotic, stocked) and those species
    collected from the Lake Agassiz Plain ecoregion during the 1993-1994 study period..

 B. Site specific IB1 scores for the stations sampled in the Lake Agassiz Plain ecoregion.

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Appendix A. Current and historic fish species found in the Red River Basin (native, exotic, stocked) and those
species collected from the Lake Agassiz Plain ecoregion. (An X indicates species found during the 1993-1994
ecoregion study). This species list provided by Todd Koel, Ph.D. candidate, North Dakota State University.
FAMILY SPECIES
Acipensendae - Sturgeon
Acipenser fulvescens
Amiidae - Bowfin
Amia calya
Cottidae - Sculpins
Cottus bairdi
Catostomidae - Suckers
Carpiodes carpio
Carpiodes cyprinus
Catostomus commersoni
Hypentelium nigricans
Ictiobus cvprinellus
Moxostoma anisurum
Moxostoma ervthrurum
Moxostoma macrolepidotum
Moxostoma valenciennesi
Centrarchidae - Sunfishes
Ambloplites rupestris
Lepomis cyanellus
Lepomis gibbosus
Lepomis humilis
Lepomis macrochirus
COMMON NAME MINNESOTA NORTH DAKOTA

lake sturgeon

bowfin

mottled sculpin

river carpsucker
quillback X X
white sucker X X
northern hog sucker
bigmouth buffalo X X
silver redhorse X X
golden redhorse X X
shorthead redhorse X X
greater redhorse X

rock bass X X
green sunfish X
pumpkinseed X
orangespotted sunfish X
bluegill X
RED RIVER








X
X

X
X
X
X


X



X
Vlicropterus dolomieu
                                   ;>mallmouth bass

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                                                    Minnesota-North Dakota Ecoregion

FAMILY SPECIES
Micropterus salmoides
Pomoxis annularis
Pomoxis nigromaculatus
Cyprmidae - Minnows
Campostoma anomalum
Campostoma oligolepis
Cvprinella spiloptera
Cvprinus carpio
Hvbognathus hankinsoni
Luxilus cornutus
Macrhybopsis storeriana
Margariscus margarita
Nocomis biguttatus
Notemieonus crvsoleucas
Notropis anogenus
Notropis atherinoides
Notropis blennius
Notropis dorsalis
Notropis heterodon
Notropis heterolepis
Notropis hudsonius
Notropis rubellus
Notropis stramineus
Notropis texanus
Notropis volucellus
Phoxmus cos
COMMON NAME
largemouth bass
white crappie
black crappie

central stoneroller
largescale stoneroller
spotfm shiner
common carp
brassy minnow
common shiner
silver chub
pearl dace
hornyhead chub
golden shiner
pugnose shiner
emerald shiner
river shiner
bigmouth shiner
blackchm shiner
blacknose shiner
spottail shiner
rosyface shiner
sand shiner
weed shiner
mimic shiner
northern redbelly
dace
MINNESOTA NORTH DAKOTA RED RIVER
X

XXX


X
XXX
XXX
X
XXX
X
X X
XXX


XXX
X
X X

X
XXX
X X
XXX


X X
Phoxmus neogaeus
finescale dace

-------

FAMILY SPECIES
Pimephales notatus
Pimephales promelas
Platvgobio gracilus
Rhinichthvs atratulus
Rhimchthys cataractae
Semotilus atromaculatus
Cyprinodontidae - Killifishes
Fundulus diaphanus
Esocidae - Pikes
Esox lucius
Esox masduinongy
Gadidae - Codfishes
Lota lota
Gasterosteidae - Sticklebacks
Culaea mconstans
Hiodontidae - Mooneyes
Hiodon alosoides
Hiodon tergisus
Ictalundae - Bullhead Catfishes
Ameiurus melas
Ameiurus natalis
Ameiurus nebulosus
Iclalurus punctatus
Noturus 11 j\ us
Noturus g\nnus
COMMON NAME
bluntnose minnow
fathead minnow
flathead chub
blacknose dace
longnose dace
creek chub

banded killifish

northern pike
muskellunge

burbot

brook stickleback

goldeye
mooneye

black bullhead
yellow bullhead
bro\vn bullhead
channel catfish
stonecat
tadpole madtom
MINNESOTA NORTH DAKOTA RED RIVER
X X
XXX

X X
X X
XXX

X

XXX


X X

X X

X X
X X

XXX

X
XXX
XXX
X X

-------
                                                                 Minnesota-North Dakota Ecoregion
FAMILY SPECIES
                                     COMMON NAME
                       MINNESOTA  NORTH DAKOTA      RED RIVER
Lepisosteidae - Gars




   Lepisosteus osseus






Percichthyidae - Temperate Basses




   Morone chrvsops
Percidae - Perches




    Etheostoma caeruleum




    Etheostoma exile




    Etheostoma microperca




    Etheostoma nigrum




    Perca flavescens




    Percina caprodes




    Percina maculata




    Percina shumardi




    Stizostedion canadense




    Stizostedion vitreum






Percopsidae - Trout-perch




    Percopsis omiscomavcus






Petromyzontidae - Lamprey




    Ichthvomvzon castaneus




    Ichthvomvzon unicuspis






Salmonidae - Trouts




    Coreaonus artedi




    Coregonus clupeaformis




    Oncorhvnchiis m\ kiss




    Salmo  trutta
longnose gar
white bass
rainbow darter
iowa darter
least darter
johnny darter
yellow perch
logperch
blackside darter
river darter
sauger
walleye

X X

X X
X X
X
X X

X
X X



X
X

X

X
X
trout-perch











chestnut lamprey




silver lamprey
ciscoe




lake \\hitefish




rambou trout




brown trout
X











X




X
                                              X

-------
Lake Agassiz Plain
   FAMILY SPECIES                  COMMON NAME      MINNESOTA  NORTH DAKOTA     RED RIVER








      Salvelinus fontinalis              brook trout






   Sciaenidae - Drums




      Aplodinotus grunniens            freshwater drum             XX                X






   Umbridae - Mudminnows




      Umbra limi                      central mudminnow          X






   Total Number of Species                      85                52               46                36

-------
                                                              Minnesota-North Dakota Ecoregion
 Appendix B:  Site specific Index of Biotic Integrity scores for each of the stations sampled in the Lake Agassiz Plain
ecoregion.  Stations are sorted by state and field number.  Total IBI scores with an asterisk have been low score
modified (see page 47 for an explanation of low score modifications)
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 93-400                 Drainage Area:  70 mi2                       Date: 7-20-1993

  Site:  MN: Kittson Co: Joe River at CR 16/intersection, 4 mi ENE St. Vincent, T139R50S34.
  Long: 97° 07'47" Lat: 48° 59'30"
METRIC (for headwater streams <200 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
3
48.6%
0.907
1
7.8%
92.2%
0.0%
51.4%
0
37.1%
0.35
0.0%,
IBI SCORE
IBI
SCORE
MOD.
1
3
5
1
1
1
1
1
1
1
1
5
22*

-------
Lake Agassiz Plain
   LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

   Station Number: 93-401                 Drainage Area:  438 mi2                      Date:  7-20-1993

   Site: MM: Kittson Co: South Branch Two Rivers at Albin bridge, 3 mi SE of Hallock, T161 R 48 S 32.
   Long: 96° 53'44" Lat: 48° 43' 54"
ACTUAL
METRIC (for moderate size streams 200- 1 500 mi2) OBSERVATION
1. TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI SCORE
11
3
0.793
4
18.1%
14.0%
67.9%
36.5%
67.1%
1
2.05
0.0%

IBI
SCORE
3
1
3
3
3
5
5
3
1
1
1
5
34

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                                                        Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 93-403                Drainage Area: 224 mi2                     Date:  7-20-1993

Site:  MN: Kittson Co:  North Branch Two Rivers at SR 6 bridge, Lancaster, T 162 R 48 S 13.
Long: 96° 49'03" Lat: 48° 51'34"
METRIC (for moderate size streams 200-1500 mi:)
1. TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
6
0
0.914
3
0.0%
44.4%
55.6%
40.4%
78.9%
1
2.28
0.0%
IBI SCORE
IBI
SCORE
3
1
5
1
1
3
3
3
1
1
1
5
28

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Lake Agassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 93-404               Drainage Area: 145 mi2                  Date: 7-20-1993

  Site: MN: Kittson Co: North Branch Two Rivers at TR bridge, 2 mi NE of Lancaster, T 162 R47 S 9.
  Long:  96°45' 16" Lat: 48°52'30"	
                                                    ACTUAL             IBI
  METRIC (for headwater streams <200 mi2)	OBSERVATION	SCORE	

  1. TOTAL NUMBER OF SPECIES                             8                3

  2. PROPORTION OF HEADWATER SPECIES                    38.9%            3

  3. EVENNESS                                            0.911             5

  4. NUMBER OF MINNOW SPECIES                           3                1

  5. PROPORTION OF OMNIVORE BIOMASS                    37.5%            3

  6. PROPORTION OF INSECTIVORE BIOMASS                  62.5%            5

  7. PROPORTION OF SIMPLE LITHOPHILS                     41.7%            3

  8. PROPORTION OF TOLERANT SPECIES                     56.3%            1

  9. NUMBER OF SENSITIVE SPECIES                         2                3

   10. PROPORTION OF PIONEER SPECIES                      27.1%            5

   11. NUMBER OF INDIVIDUALS/METER                       2.68              1

   12. PERCENT DELT                                       0.4%             5

                                      TOTAL IBI SCORE                     38

-------
                                                        Minnesota-North Dakota Ecoregion
 LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

 Station Number: 93-405                Drainage Area:  118 mi2                    Date: 7-20-1993

 Site: MM: Kittson Co: Middle Branch Two Rivers between TR 59 & 15, 3 mi N of Lake Bronson, T 161 R 47
S 1. Long: 96°40'35" Laf. 48° 47'21"
METRIC (for headwater streams <200 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
•}
3
69.9%
0.585%
1
0.1%
99.9%
0.0%
30.1%
0
0.6%
2.05
0.0%
SCORE
IBI
SCORE
MOD.
1
5
1
1
1
1
1
1
1
1
1
5
20*

-------
Lake Agassiz Plain
   LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

   Station Number: 93-406                 Drainage Area: 420 mi2                      Date: 7-20-1993

   Site: MM: Kittson Co: South Branch Two Rivers at CR 15/HWY 59 bridge, Lake Bronson, T 161 R 46 S 31.
  Long:  96° 40' 04" Lat: 48° 43' 54"
METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
4
10. NUMBER OF SENSITIVE SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
12
3
0.624
4
5.5%
39.4%
55.2%
67.1%
67.7%
0
2.48
0.0%
SCORE
IBI
SCORE
3
1
3
3
1
3
o
j
5
1
1
1
5
30

-------
                                                       Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 93-407                Drainage Area: 270 mi2                     Date: 7-20-1993

Site:  MN: Kittson Co: South Branch Two Rivers at SR 11 bridge , 6 mi NE Karlstad, T 160 R 45 S 36.
Long: 96° 23' 12"  Laf. 48° 38' 33"
METRIC (for moderate size streams 200-1500 mi:)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECT1VORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
7
1
0.763
^
3
0.0%
52.3%
47.7%
21.6%
68.9%
0
2.96
0.0%
IBI SCORE
IBI
SCORE
3
1
3
1
1
3
*i
j
1
1
1
1
5
24

-------
Lake Agassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 93-416                 Drainage Area:  170 mi2                     Date:7-22-1993

  Site: MN: Marshall  Co:  Snake River at 5th Street bridge, 0.125 mi NW of Warren, T 155 R 48 S 36.
  Long: 96° 46' 02" Lat: 48° 12' 02"
ACTUAL
METRIC (for headwater streams <200 mi2) OBSERVATION
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE L1THOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI SCORE
6
34.9%
0.671
4
77.3%
22.7%
11.1%
65.1%
2
57.1%
2.52
0.0%

IBI
SCORE
3
3
3
3
1
1
1
1
3
3
1
5
28

-------
                                                        Minnesota-North Dakota Ecoregion
 LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

 Station Number: 93-417                Drainage Area:  255 mi2                     Date: 7-22-1993

 Site: MM: Marshall Co:  Middle River at CR 4 bridge, 0.25 mi E of Argyle, T 156 R 48 S 15. Long: 96° 48'
40"Lat: 48° 20' 16"
METRIC (for moderate size streams 200-1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
10
2
0.671
4
0.0%
99.2%
0.8%
31.7%
70.4%
1
4.80
0.0%
SCORE
IBI
SCORE
->
j
1
3
3
1
1
1
3
1
1
3
5
26

-------
Lake Agassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 93-418                 Drainage Area: 205 mi2                      Date: 7-22-1993

  Site: MN: Marshall Co: Tamarac River at TR 9 bridge, 9 mi SE of Stephan, T 157 R 47 S 14. Long: 96° 41'
  31"Lat: 48° 24'56"
METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL IBI
OBSERVATION SCORE
MOD.
5 1
1 1
0.406 1
0 1
0.0% 1
15.0% 1
85.0% 1
2.3% 1
83.7% 1
0 1
1.72 1
0.0% 5
IBI SCORE 16*

-------
                                                        Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number:  93-419                Drainage Area: 220 mi2                      Date: 7-22-1993

Site:  MN: Marshall  Co: Middle River at Old Mill State Park, 11 mi E of Argyle, T 156 R 46 S 4.
Long: 96° 35'08" Lat: 48° 21'32"
METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF P1SC1VORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
12
2
0.802
7
0.0%
66.1%
33.9%
25.6%
51.2%
2
6.56
0.0%
IBI SCORE
IBI
SCORE
5
1
5
5
1
1
->
;>
1
3
3
3
5
36

-------
Lake Agassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 93-420                Drainage Area: 70 mi2                       Date:7-22-1993

  Site: MN: Marshall Co: Snake River at CR 136 bridge, 7.5 mi E of Warren, T 155 R 46 S 29. Long: 96° 36'
  28"Lat: 48° 12'43"
METRIC (for headwater streams <200 mi2)
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
9
37.0%
0.598
5
61.6%
38.4%
6.0%
61.4%
1
56.5%
3.68
0.0%
SCORE
IBI
SCORE
5
3
1
5
•5
3
3
1
1
1
3
1
5
32

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                                                          Minnesota-North Dakota Ecoregion
 LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

 Station Number:93-421                 Drainage Area: 60 mi2                      Date:  7-23-1996

 Site:  MN: Pennington  Co:  Black River at CR 55 bridge, 10.25 mi W of St. Hilaire, T 152 R 45 S 4.  Long: 96°
25' 51" Lat: 48° 00'28"
METRIC (for headwater streams <200 mi2)
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
4
85.7%
0.73
2
0.0%
100.0%
24.5%
14.3%
2
0.0%
0.98
0.0%
IBI SCORE
IBI
SCORE
3
5
3
1
5
5
1
1
3
5
1
5
3-8

-------
Lake Agassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 93-422                Drainage Area: 271 mi2                      Date:  7-23-1993

  Site: MN: Red Lake Co: Lost River at TR bridge, 2 mi NE of Brooks, T 151 R 42 S 36. Long: 95° 58'37"
  Lat: 47° 50' 53"
METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
13
6
0.718
6
15.2%
43.9%
40.9%
8.4%
35.3%
3
2.38
0.0%
IBI SCORE
IBI
SCORE
5
3
3
3
3
3
3
1
3
3
1
5
36

-------
                                                         Minnesota-North Dakota Ecoregion
 LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

 Station Number:93-423                 Drainage Area:  174 mi2                     Date: 7-23-1993

 Site: MM: Red Lake Co: Hill River, CR at junction 92, 2.5 mi E Brooks, T 150 R 41 S 17.  Long: 95° 56'55"
Lat: 47° 49' 08"
METRIC (for headwater streams <200 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
14
19.6%
0.782
7
31.8%
50.6%
25.8%
40.2%
2
27.3%
3.88
0.0%
SCORE
IBI
SCORE
5
1
->
j
5
5
3
1
3
3
5
3
5
42

-------
Lake Agassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number:93-424                 Drainage Area: 100 mi2                     Date:  7-23-1993

  Site: MN: Red Lake Co: Poplar River at CR 7 bridge, 3 mi S of Brooks, T 150 R 42 S 34. Long: 96° 01' 46"
  Lat: 47° 46' 28"
METRIC (for headwater streams <200 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
8
19.0%
0.776
4
0.3%
99.7%
13.9%
44.3%
0
20.3%
1.58
0.0%
IBI SCORE
IBI
SCORE
3
1
3
3
5
5
1
3
1
5
1
5
36

-------
                                                        Minnesota-North Dakota Ecoregion
 LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

 Station Number: 93-425                Drainage Area:  140 mi2                    Date:  7-23-1993

 Site: MN: Red Lake  Co:  Lower Badger Creek at CR 11 bridge, 3 mi SW of Terrebonne, T 150 R 43 S 18.
Long:  96° 12'34" Lat: 47° 49" 03"
METRIC (for headwater streams <200 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
9
30.5%
0.778
5
38.5%
61.5%
35.8%
53.0%
0
17.9%
3.02
0.0%
SCORE
IBI
SCORE
->
3
3
3
3
3
5
3
3
1
5
1
5
38

-------
Lake Agassiz Plain
   LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

   Station Number: 94-001                 Drainage Area: 375 mi2                       Date: 6-22-1994

   Site: MM: Traverse Co: West Branch Mustinka Creek, 0.15 mi west of CR 13, T 127 R 45 S 30. Long: 96°
  22'01" Lat: 45° 47' 19"
METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL IBI
OBSERVATION SCORE
MOD.
6 1
1 1
0.674 3
3 1
0.0% 1
61.5% 1
38.5% 1
13.5% 1
57.0% 3
0 1
4.58 3
0.0% 5
SCORE 22*

-------
                                                        Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-002                Drainage Area:  190 mi2                    Date:  6-22-1994

Site:  MM:  Wilkin Co: Rabbit River at TR bridge, 2.25 mi W of River Center, T 130 R 45 S 8.
Long: 96° 21' 14" Lat: 46° 04' 45"
METRIC (for headwater streams <200 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
6
6.7%
0.422
0
98.7%
1 .3%
86.7%
85.3%
0
0.0%
0.73
0.0%
IBI SCORE
IBI
SCORE
->
j
1
1
1
1
1
5
1
1
5
1
5
26

-------
Lake Aeassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 94-004                 Drainage Area:  107 mi2                     Date:  6-23-1994

  Site: MN: Wilkin Co: South Branch Buffalo River upstream of Wilkin CR 188, T 136 R 46 S 18.
  Long: 96° 36' 12" Lat: 46° 36' 53"
METRIC (for headwater streams <200 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMN1VORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
3
0.0%
0.527
0
99.6%
0.4%
16.1%
96.8%
0
0.0%
0.31
0
IB1 SCORE
IBI
SCORE
MOD.
1
1
1
1
1
1
1
1
1
1
1
5
16*

-------
                                                      Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY




Station Number: 94-005                Drainage Area: 120 mi2                    Date:6-23-1994




Site:  MN: Clay Co: Whiskey Creek at CR 57, T 137 R 47 S 13. Long: 96° 33'35" Lat: 46° 41' 19"
METRIC (for headwater streams <200 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
4
0.0%
0.726
2
99.6%
0.4%
64.7%
100.0%
0
1 1 .8%
0.15
0.0%
IB1 SCORE
IBI
SCORE
MOD.
1
1
3
1
1
1
1
1
1
1
1
5
18*

-------
Lake Agassiz Plain
   LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

   Station Number: 94-006                Drainage Area: 137 mi2                     Date:6-24-1994

   Site: MN: Clay Co: Stony Creek at Cty. Rd. 21, 1.5 mi from Baker, T 138 R 46 S 31.  Long: 96° 32' 49"
   Lat:46°43'08"
METRIC (for headwater streams <200 mi2)
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
3
0.0%
1
0
2 1 .0%
0.0%
66.7%
33.3%
0
0.0%
0.03
0.0%
1BI SCORE
IBI
SCORE
MOD.
1
1
5
1
1
1
1
3
1
1
1
5
22*

-------
                                                        Minnesota-North Dakota Ecoregion
 LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

 Station Number: 94-007                 Drainage Area: 420 mi2                      Date: 6-28-1994

 Site: MN: Kittson Co:  South Branch Two Rivers at CR 15/HWY 59 bridge, Lake Bronson, T 161 R 46 S 31.
Long:  96° 40' 04" Lat: 48° 43' 54"
METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IB1
ACTUAL
OBSERVATION
13
3
0.653
4
2.8%
47.5%
49.7%
66.6%
59.1%
1
7.42
0.0%
SCORE
1BI
SCORE
5
1
i
j
••>
j
1
3
3
5
1
1
5
5
36

-------
Lake Agassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 94-008                 Drainage Area: 255 mi2                      Date: 6-29-1993

  Site: MM: Marshall Co: Middle River at CR 4 bridge, 0.25 mi E of Argyle, T 156 R 48 S 15.  Long:  96° 48'
  40"Lat: 48° 20' 16"
METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
13
4
0.602
6
13.9%
72.5%
13.7%
36.3%
88.2%
2
4.48
0.5%
SCORE
IBI
SCORE
5
3
3
3
3
1
1
3
1
3
3
5
34

-------
                                                        Minnesota-North Dakota Ecoregion
 LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

 Station Number: 94-009                 Drainage Area: 420 mi2                      Date: 6-29-1994

 Site: MM: Kittson Co: South Branch Two Rivers at CR 15/HWY 59 bridge, Lake Bronson, T 161 R 46 S 31.
Long:  96°40'04"Lat:  48° 43'54"
METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
11
*>
j
0.747
3
5.1%
28.4%
66.5%
6 1 .4%
61.4%
1
2.72
0.0%
IBI SCORE
IBI
SCORE
•>
j
1
3
1
1
5
5
5
1
1
1
5
32

-------
Lake Agassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 94-010                Drainage Area:  220 mi2                      Date: 6-29-1994

  Site: MN: Roseau Co: South Fork Roseau River at Twp. Rd. 0.75 mi E of Hwy 89, T 161 R 40 S 13.
  Long:  95° 41'43"  Lat: 48° 35'03"
METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
13
3
0.763
7
17.8%
47.7%
34.5%
21.7%
34.1%
2
4.23
0.5%
SCORE
IBI
SCORE
5
3
3
5
3
3
3
1
3
3
3
5
40

-------
                                                   Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY




Station Number: 94-011               Drainage Area: 65 mi2                   Date: 08-01-1994




Site: MM:  Clay Co: Hay Creek, 1 mi S of CR71, T 138 R46 S 11. Long: 96° 26'47" Lat: 46° 46' 33"
METRIC (for headwater streams <200 mi2)
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
ACTUAL
OBSERVATION
6
24.2%
0.687
IBI
SCORE
->
j
1
3
4. NUMBER OF MINNOW SPECIES                           2                1




5. PROPORTION OF OMNIVORE BIOMASS                     47.7%             3




6. PROPORTION OF INSECTIVORE BIOMASS                  52.3%             3




7. PROPORTION OF SIMPLE L1THOPHILS                     53.5%             3




8. PROPORTION OF TOLERANT SPECIES                      74.7%             1




9. NUMBER OF SENSITIVE SPECIES                          0                1




10. PROPORTION OF PIONEER SPECIES                       16.2%             5




11. NUMBER OF INDIVIDUALS/METER                       0.94              1




12. PERCENT DELT                                       0.0%              5




                                    TOTAL IBI SCORE                     30

-------
Lake Agassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 94-012                Drainage Area: 185 mi2                     Date: 08-02-1994

  Site: MN: Clay Co: South Branch Wild Rice River, E of CSAH 27 bridge, T 142 R 45 S 16. Long: 96° 24' 08"
  Lat:47°07'01"
METRIC (for headwater streams <200 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
20
17.0%
0.744
10
32.7%
58.8%
49.9%
49.5%
7
27.0%
3.52
00%
IBI SCORE
IBI
SCORE
5
1
3
5
5
3
3
3
5
5
1
5
44

-------
                                                        Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-013                Drainage Area:  207 mi2                      Date: 08-02-1994

Site:  MN: Norman Co: South Branch Wild Rice River, W of CR 136 bridge, T 143 R 46 S 30.
Long: 96° 33' 35" Lat: 47° 10' 15"
METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
21
10
0.659
11
20.8%
9.4%
69.8%
20.5%
48.4%
8
1.91
0.0%
SCORE
IBI
SCORE
5
5
3
5
5
5
5
1
3
5
1
5
48

-------
Lake Agassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 94-014                Drainage Area: 223 mi2                     Date: 08-02-1994

  Site: MM: Norman Co: Marsh River, N of CR129 bridge, T 146 R 48 S 34. Long: 96° 45'58" Lat: 47° 24'
  44"
METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
17
5
0.749
9
36.2%
26.5%
37.3%
10.1%
59.7%
3
3.08
0.2%
SCORE
IBI
SCORE
5
3
3
5
3
5
3
1
3
3
1
5
40

-------
                                                         Minnesota-North Dakota Ecoregion
 LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

 Station Number: 94-015                Drainage Area:  380 mi2                     Date:  08-03-1994

 Site: MN: Clay Co: Buffalo River at Buffalo State Park, 0.2 mi below dam, T 139 R 46 S 10.  Long: 96° 28'
09" Lat: 46° 51' 44"
METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECT1VORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE L1THOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IB1
ACTUAL
OBSERVATION
26
10
0.751
10
15.0%
58.9%
26.1%
27.8%
44.6%
11
0.96
0.0%
SCORE
IBI
SCORE
5
5
3
5
3
3
1
1
3
5
1
5
40

-------
Lake Agassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 94-016                Drainage Area:  1,524 mi2                     Date: 09-07-1994

  Site: MN: Norman  Co: Wild Rice River at Leon Burgen Clubhouse, 0.5 mi E of CR 6, T 143 R 48 S 14.
  Long: 96° 43' 14" Lat: 47° 11'54"
METRIC (for large streams >1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF LARGE RIVER SPECIES
3. PROPORTION OF ROUND BODIED SUCKERS
4. EVENNESS
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPH1LS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
23
28.7%
15.4%
0.856
39.1%
48.6%
12.3%
21.7%
33.6%
9
0.48
0.0%
IBI SCORE
IBI
SCORE
5
3
1
5
3
3
1
1
3
5
1
5
36

-------
                                                       Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-017                Drainage Area: 1,613 mi2                    Date:  09-07-1994

Site:  MM: Norman  Co:  Wild Rice River, 0.5 mi W of end of CR 109, T 146 R 49 S 12. Long:  96° 50'09"
Lat: 47° 19'01"
METRIC (for large streams > 1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF LARGE RIVER SPECIES
3. PROPORTION OF ROUND BODIED SUCKERS
4. EVENNESS
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
21
48.1%
7.5%
0.787
18.0%
5 1 .6%
30.3%
11.3%
23.3%
9
1.77
00%
IBI SCORE
IBI
SCORE
5
5
1
3
3
3
->
j
1
5
5
1
5
40

-------
Lake Agassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 94-018                 Drainage Area: 16,753 mi2                   Date: 09-07-1994

  Site: MM: Norman Co:  Red River, 0.5 mi W of end of CR 109, T 144 R 48 S 1. Long: 96°49'45" Lat: 47°
  19' 35"
METRIC (for large streams >1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF LARGE RIVER SPECIES
3. PROPORTION OF ROUND BODIED SUCKERS
4. EVENNESS
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
24
37.5%
12.5%
0.868
22.5%
20.5%
56.8%
17.5%
18.8%
9
0.32
0.0%
SCORE
IBI
SCORE
5
3
1
5
5
5
3
1
5
5
1
5
44

-------
                                                       Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-019                Drainage Area: 1025 mi2                     Date: 09-08-1994

Site:  MN: Clay Co: Buffalo River at CR 26, 2.5 mi E of Kragnes, T 141 R 48 S 36. Long: 96° 41'26"
Lat: 46° 58'39"
METRIC (for moderate size streams 200-1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
21
5
0.872
5
14.3%
51.5%
34.0%
34.0%
20.6%
7
0.39
0.0%
IBI SCORE
IBI
SCORE
5
3
5
•>
j
3
3
3
-}
j
5
5
1
5
44

-------
Lake Agassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 94-020                 Drainage Area: 3,890 mi2                    Date: 09-08-1994

  Site: MN: Wilkin  Co:  Red River, Downstream of Hwy. 210, below power plant, T 133 R 47 S 33. Long: 96°
  36' 17" Lat: 46° 17'40"
METRIC (for large streams > 1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF LARGE RIVER SPECIES
3. PROPORTION OF ROUND BODIED SUCKERS
4. EVENNESS
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
18
23.6%
4.4%
0.527
50.6%
29.9%
19.1%
5.0%
8.7%
5
0.69
0.3%
SCORE
IBI
SCORE
5
3
1
1
1
5
1
1
5
3
1
5
32

-------
                                                       Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY




Station Number: 94-021                Drainage Area:  3895 mi2                    Date: 09-20-1994




Site:  MN: Wilkin  Co: Red River, 0.5 mi W of Hwy 75, T 133 R 47 S 21. Long: 96° 36'03" Lat: 46° 19'36"
METRIC (for large streams > 1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF LARGE RIVER SPECIES
3. PROPORTION OF ROUND BODIED SUCKERS
4. EVENNESS
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
16
31.3%
46.1%
0.711
17.1%
2.1%
80.8%
49.6%
12.2%
5
0.23
0.0%
IB1 SCORE
IBI
SCORE
•>
j
•1
j
5
3
••>
j
5
5
->
_>
5
3
1
5
44

-------
Lake Agassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 94-022                 Drainage Area:  1925 mi2                     Date: 09-09-1994

  Site: MN: Wilkin Co: Ottertail River, adjacent to CR 14, T 132 R 46 S 18.  Long:  96° 29'32" Lat: 46° 15'
  04"
METRIC (for large streams > 1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF LARGE RIVER SPECIES
3. PROPORTION OF ROUND BODIED SUCKERS
4. EVENNESS
5. PROPORTION OF PISC1VORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION,
25
3.4%
9.1%
0.785
2.8%
70.9%
25.8%
17.3%
39.0%
8
1.25
0.3%
SCORE
IBI
SCORE
5
1
1
3
I
1
1
1
3
5
1
5
28

-------
                                                        Minnesota-North Dakota Ecoregion
 LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

 Station Number: 94-506                Drainage Area: 3,580 mi2                    Date: 09-03-1994

 Site: MN: Red Lake Co:  Red Lake River, USGS site at Red Lake Falls, field # 47533109, T 151 R 44 S 13.
Long:  96° 14'46" Lat: 47° 55'31"
METRIC (for large streams >1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF LARGE RIVER SPECIES
3. PROPORTION OF ROUND BODIED SUCKERS
4. EVENNESS
5. PROPORTION OF PISC1VORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
23
5.4%
19.4%
0.79
12.5%
7.1%
80.4%
43.6%
12.4%
9
1.40
0.3%
SCORE
IBI
SCORE
5
1
1
3
3
5
5
3
5
5
\
5
42

-------
Lake Agassiz Plain
   LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

   Station Number:  94-507                 Drainage Area: 380 mi2                      Date:  08-12-1994

   Site: MN: Clay Co: Buffalo River, USGS site near Buffalo River State Park, Field #46514909, T 139 R 46 S
  11. Long: 96° 28'04" Lat: 46° 51'49"
METRIC (for moderate size streams 200-1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
20
9
0.602
10
12.7%
40.0%
47.4%
27.0%
11.8%
9
3.23
1 .6%
IBI SCORE
IBI
SCORE
5
5
3
5
••>
j
3
3
1
5
5
3
3
44

-------
                                                        Minnesota-North Dakota Ecoregion
 LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

 Station Number: 94-508                 Drainage Area: 446 mi2                      Date: 08-22-1994

 Site: MN: Kittson  Co:  South Branch Two Rivers, USGS site at Lake Bronson, field # 5094000, T 161 R 46 S
30 Long: 96° 39' 50" Lat:  48° 43' 50"
METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
9
4
0.699
4
0.0%
33.1%
66.9%
32.9%
36.6%
2
5.19
1.3%
IBI SCORE
IBI
SCORE
3
3
•}
3
3
1
3
5
3
3
1
3
3
34

-------
Lake Agassiz Plain
   LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

   Station Number:  94-509                 Drainage Area: 218 mi2                      Date: 08-10-1994

   Site: MN: Polk Co: Snake River, USGS site near Alvarado, field # 5085900, reach 3, T 154 R 49 S 8. Long:
  97° 00' 20" Lat:  48° 11' 50"
METRIC (for moderate size streams 200-1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
6
1
0.916
2
0.0%
13.9%
86.1%
15.4%
73.1%
0
0.17
3.8%
IBI SCORE
IBI
SCORE
3
1
5
1
1
5
5
1
1
1
1
•^
j
28

-------
                                                        Minnesota-North Dakota Ecoregion
 LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

 Station Number: 94-510                Drainage Area: 218 mi2                      Date: 08-10-1994

 Site: MN: Polk Co:  Snake River, USGS site near Alvarado, field # 5085900, reach 2, T 154 R 49 S 8. Long:
97° 00'20" Lat: 48° 11' 50"
METRIC (for moderate size streams 200-1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
7
2
0.863
2
0.0%
65.0%
35.0%
19.6%
82.1%
0
0.37
1.8%
SCORE
IBI
SCORE
-i
j
1
5
1
1
3
3
1
1
1
1
3
24

-------
Lake Agassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 94-511                 Drainage Area: 218 mi2                       Date: 08-10-1994

  Site: MM: Polk Co: Snake River, USGS site near Alvarado, field # 5085900, reach 1, T 154 R 49 S 8. Long:
  97° 00' 20" Lat:  48° 11' 50"
METRIC (for moderate size streams 200-1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
6
1
0.912
2
0.0%
47.6%
52.4%
21.4%
76.2%
0
0.28
0.0%
IBI SCORE
IBI
SCORE
->
j
1
5
I
1
3
3
1
1
1
1
5
26

-------
                                                        Minnesota-North Dakota Ecoregion
 LAKE AGASS1Z PLAIN BIOCRITERIA STUDY

 Station Number: 94-512                 Drainage Area: l,358mi2                     Date: 08-24-1994

 Site: MN: Red Lake Co:  Clearwater River, USGS site at Red Lake Falls, field # 0507850, T 151 R 44 S 22.
Long: 96° 16'25" Lat: 47° 53' 15"
METRIC (for moderate size streams 200-1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC 1NSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECT1VORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
12
5
0.856
6
74.5%
0.3%
25.2%
46.4%
18.6%
6
1.22
2.7%
SCORE
IBI
SCORE
T
J
3
5
3
1
5
1
3
5
5
1
3
38

-------
Lake Agassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 94-513               Drainage Area: 5,270 mi2                  Date: 08-31-1994

  Site: MN: Polk Co: Red Lake River, USGS site at Crookston, field # 05079000, T 150 R 46 S 30. Long: 96°
  36'33" Lat: 47° 46'32"	
                                                    ACTUAL             IBI
  METRIC (for large streams >1500 mi2)	OBSERVATION	SCORE	

  1. TOTAL NUMBER OF SPECIES                             20                5

  2. PROPORTION OF LARGE RIVER SPECIES                   3.5%              1

  3. PROPORTION OF ROUND BODIED SUCKERS                0.5%              1

  4. EVENNESS                                            0.526              1

  5. PROPORTION OF PISCIVORE BIOMASS                     69.2%             1

  6. PROPORTION OF OMNIVORE BIOMASS                     15.8%             5

  7. PROPORTION OF INSECTIVORE BIOMASS                  14.8%             1

  8. PROPORTION OF SIMPLE LITHOPHILS                     1.6%              1

  9. PROPORTION OF TOLERANT SPECIES                      62.8%             1

  10. NUMBER OF SENSITIVE SPECIES                         7                  5

  11. NUMBER OF INDIVIDUALS/METER                       2.74               5

  12. PERCENT DELT                                       0.3%              5

                                      TOTAL IBI SCORE                      32

-------
                                                        Minnesota-North Dakota Ecoregion
 LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

 Station Number: 94-532                Drainage Area: 1,880 mi2                    Date: 08-14-1994

 Site: MM: Wilkin  Co:  Bois de Sioux River, USGS near Doran, CSAH 6, field # 05051200, T 131 R 47 S 21.
Long:  96° 34' 46" Laf. 46° 09' 08"
METRIC (for large streams >1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF LARGE RIVER SPECIES
3. PROPORTION OF ROUND BODIED SUCKERS
4. EVENNESS
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
14
24.8%
0.1%
0.471
0.0%
90.5%
9.5%
1.4%
70.9%
2
4.73
0.6%
IBI SCORE
IBI
SCORE
-t
j
3
1
1
1
1
1
1
1
1
5
5
24

-------
Lake Agassiz Plain

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 93-408 Drainage Area:

160 mi2

Date: 7-2 1-1993
Site: ND: Pembina Co: Tongue River below Renwick Dam, 6 mi W Cavalier.
Long: 97° 43' 40.2" Lat: 48° 46' 40".
METRIC (for headwater streams <200 mi2)
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
8
9.5%
0.595
3
85.5%
12.6%
79%
84.8%
0
11.4%
2.1
0
SCORE
IBI
SCORE
3
1
3
1
1
1
5
1
1
5
1
5
28

-------
                                                   Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 93-409               Drainage Area: 295 mi2                     Date: 7-21-1993

Site: ND: Pembina Co: Tongue River at Hwy 18, 3.25 mi SW Bathgate.
Long: 97° 33'21.6" Lat: 48° 51'06".

                                                  ACTUAL             IBI
METRIC (for moderate size streams 200-1500 mi2)	OBSERVATION	SCORE	

1.  TOTAL NUMBER OF SPECIES                            7                  3

2.  NUMBER OF BENTHIC INSECTIVORES                    1                  1

3.  EVENNESS                                           0.812               5

4.  NUMBER OF MINNOW SPECIES                          3                  1

5.  PROPORTION OF PISCIVORE BIOMASS                   0                  1

6.  PROPORTION OF OMNIVORE BIOMASS                   29.4%              5

7.  PROPORTION OF INSECTIVORE BIOMASS                 .706                5

8.  PROPORTION OF SIMPLE LITHOPHILS                    37.5%              3

9.  PROPORTION OF TOLERANT SPECIES                     70.5%              1

10. NUMBER OF SENSITIVE SPECIES                        0                  1

11. NUMBER OF INDIVIDUALS/METER                      1.76                1

12. PERCENT DELT                                      1.1%               3

                                     TOTAL IBI SCORE                      30

-------
Lake Agassiz Plain

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 93-4 1 0 Drainage Area: 3300
Site: ND: Pembina Co: Pembina River, County Road 12 bridge, 0.
Long: 97° 45' 07.1" Lat: 48° 55' 59".
METRIC (for large streams >1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF LARGE RIVER SPECIES
3. PROPORTION OF ROUND BODIED SUCKERS
4. EVENNESS
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT

mi2
5 mi N Leroy.
ACTUAL
OBSERVATION
12
0
3%
0.64
7%
1.1%
97.5%
4.9%
20.9%
4
2.6
0
TOTAL IBI SCORE

Date: 7-2 1-1993

IBI
SCORE
3
1
1
3
1
5
5
1
5
3
3
5
36

-------
                                                        Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 93-411                 Drainage Area: 3200 mi2                     Date: 7-21-1993

Site: ND: Pembina Co: Pembina River, SH 32 bridge, 0.75 mi SW Walhalla.
Long: 97° 55' 01" Lat:  48° 54' 91".
ACTUAL
METRIC (for large streams >1 500 mi2) OBSERVATION
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.

TOTAL NUMBER OF SPECIES
PROPORTION OF LARGE RIVER SPECIES
PROPORTION OF ROUND BODIED SUCKERS
EVENNESS
PROPORTION OF PISCIVORE BIOMASS
PROPORTION OF OMNIVORE BIOMASS
PROPORTION OF INSECTIVORE BIOMASS
PROPORTION OF SIMPLE LITHOPHILS
PROPORTION OF TOLERANT SPECIES
NUMBER OF SENSITIVE SPECIES
NUMBER OF INDIVIDUALS/METER
PERCENT DELT
TOTAL 1BI SCORE
13
0
0
0.705
15.7%
17.1%
63.7%
17.2%
33.6%
4
1.44
0

IBI
SCORE
3
1
1
3
3
5
5
1
3
1
j
1
5
34

-------

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 93-412 Drainage Area: 66 mi2
Site: ND: Pembina Co: North Branch Park River, SH 32 bridge, 6 mi N Edinburg.
Long: 97° 51' 42" Lat: 48° 35' 26".
ACTUAL
METRIC (for headwater streams <200 mi2) OBSERVATION
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI SCORE
5
80.9%
0.499
3
43.7%
43.3%
8.5%
99.8%
0
18.9%
8.26
0

Date: 7-2 1-1993
IBI
SCORE
3
5
1
3
3
3
1
1
1
5
5
5
36

-------
                                                        Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 93-413                 Drainage Area: 89 mi2                      Date: 7-21-1993

Site: ND: Walsh Co: South Branch Park River, SH 32 bridge, 5.5 mi W, 1.5 mi N Park River.
Long: 97° 51' 43" Lat: 48° 24' 52".
METRIC (for headwater streams <200 mi2)
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IB1
ACTUAL
OBSERVATION
8
48.7%
0.685
5
19%
81%
53.4%
63.2%
1
23.3%
3.86
0
SCORE
IBI
SCORE
->
_>
3
3
3
5
5
•>
j
1
1
5
1
5
38

-------
Lake Aeassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 93-414                 Drainage Area: 157 mi2                      Date: 7-21-1993

  Site: ND: Walsh Co: Middle Branch Forest River, SH 32 bridge, 3 mi W Fordville.
  Long: 97° 51' 38" Lat: 48° 12' 43".
ACTUAL IBI
METRIC (for headwater streams <200 mi2) OBSERVATION SCORE
MOD.
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.

TOTAL NUMBER OF SPECIES
PROPORTION OF HEADWATER SPECIES
EVENNESS
NUMBER OF MINNOW SPECIES
PROPORTION OF OMNIVORE BIOMASS
PROPORTION OF INSECTIVORE BIOMASS
PROPORTION OF SIMPLE LITHOPHILS
PROPORTION OF TOLERANT SPECIES
NUMBER OF SENSITIVE SPECIES
PROPORTION OF PIONEER SPECIES
NUMBER OF INDIVIDUALS/METER
PERCENT DELT
TOTAL IBI SCORE
3 1
0 1
0.349 1
1 1
94.2% 1
5.8% 1
90.5% 1
98.6% 1
0 1
9.5% 1
1.48 1
0 5
16*

-------
                                                       Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 93-415                 Drainage Area: 416 mi2                     Date: 7-21-1993

Site: ND: Grand Forks Co: Forest River 2 mi N Inkster on bridge.
Long: 97° 38' 37" Lat: 48° 10' 46".
METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
12
5
0.729
8
0
40.7%
56.7%
36.8%
46.3%
2
3.07
0.3%
SCORE
IBI
SCORE
3
3
3
5
1
3
3
3
3
1
3
5
36

-------

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 93-427 Drainage Area:

148 mi2

Date: 7-23-1993
Site: ND: Grand Forks Co: North Branch Turtle River, SH 18 bridge, 3 mi N US 2 Junct.
Long: 97° 37' 25" Lat: 47° 59' 31".
METRIC (for headwater streams <200 mi2)
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
8
41.8%
0.721
4
30.6%
66.2%
45.5%
85.5%
1
40%
0.73
0
SCORE
IBI
SCORE
••>
j
3
3
3
5
5
3
1
1
3
1
5
36

-------
                                                   Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 93-428               Drainage Area: 34 mi2                     Date: 7-23-1993

Site: ND: Grand Forks Co: Little Goose River, 6 mi S, 3 mi W Larimore.
Long: 97° 41' 37" Lat: 47° 49' 01".
METRIC (for headwater streams <200 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
ACTUAL
OBSERVATION
3
22.9%
0.554
IBI
SCORE
3
1
1
4.  NUMBER OF MINNOW SPECIES                          1                 1

5.  PROPORTION OF OMNIVORE BIOMASS                   92.2%             1

6.  PROPORTION OF 1NSECT1VORE BIOMASS                 7.8%              1

7.  PROPORTION OF SIMPLE LITHOPHILS                    0                 1

8.  PROPORTION OF TOLERANT SPECIES                     98.6%             1

9.  NUMBER OF SENSITIVE SPECIES                         0                 1

10. PROPORTION OF PIONEER SPECIES                      75.7%             1

11. NUMBER OF INDIVIDUALS/METER                      1.4                1

12. PERCENT DELT                                      0                 5

                                    TOTAL IBI SCORE                      18

-------
Lake Agassiz Plain

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94- 1 0 1 Drainage Area:

220 mi2

Date: 7-26-1994
Site: ND: Walsh Co: Middle Branch Park River, .5 mi N, 5 mi E, .5 mi N Edinburg.
Long: 97° 45' 12" Lat: 48° 30' 30".
METRIC (for moderate size streams 200-1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
8
2
0.824
5
0
61.7%
38.3%
29.6%
92.1%
0
23.1
0.4%
SCORE
IBI
SCORE
3
1
5
3
1
3
3
1
1
1
5
5
32

-------
                                                   Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-102               Drainage Area: 333 mi2                    Date: 7-27-1994

Site: ND: Grand Forks Co: Turtle River, 1 mi E, .5 mi S Middle Grove Church.
Long: 97° 14' 17" Lat: 48° 00' 50".

                                                  ACTUAL            IBI
METRIC (for moderate size streams 200-1500 mr)	OBSERVATION	SCORE	

1.  TOTAL NUMBER OF SPECIES                             12                5

2.  NUMBER OF BENTHIC INSECTIVORES                    4                 3

3.  EVENNESS                                           0.549              1

4.  NUMBER OF MINNOW SPECIES                          4                 3

5.  PROPORTION OF PISCIVORE BIOMASS                     1.3%              1

6.  PROPORTION OF OMNIVORE BIOMASS                   81.4%             1

7.  PROPORTION OF INSECTIVORE BIOMASS                  17.3%             1

8.  PROPORTION OF SIMPLE LITHOPHILS                    7.4%              1

9.  PROPORTION OF TOLERANT SPECIES                    86.3%             1

10.  NUMBER OF  SENSITIVE SPECIES                         1                 1

11.  NUMBER OF  INDIVIDUALS/METER                      2.63               1

12.  PERCENT DELT                                       0                 5

                                    TOTAL IBI SCORE                      24

-------
Lake Agassiz Plain

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-103 Drainage Area: 138 mi2
Site: ND: Cass Co: Rush River, 4 mi N, 2 mi W, .5 mi N Mapleton.
Long: 97° 05' 36" Lat: 46° 58' 02".
METRIC (for headwater streams <200 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
1 0. PROPORTION OF PIONEER SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI SCORE

ACTUAL
OBSERVATION
9
0
0.184
4
88.3%
11.7%
5.9%
99.6%
0
91.6%
18.9
0


Date: 9-09- 1994

IBI
SCORE
3
1
1
3
1
1
1
1
1
1
5
5
24

-------
                                                        Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-104                 Drainage Area: 116 mi2                     Date: 9-09-1994

Site: ND: Cass Co: Rush River, 5.3 mi W, 1.2 mi N of SH 18 at Amenia.
Long: 97° 19'30" Lat: 47° 01'21".
ACTUAL
METRIC (for headwater streams <200 mi2) OBSERVATION
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12,

TOTAL NUMBER OF SPECIES
PROPORTION OF HEADWATER SPECIES
EVENNESS
NUMBER OF MINNOW SPECIES
PROPORTION OF OMNIVORE BIOMASS
PROPORTION OF INSECTIVORE BIOMASS
PROPORTION OF SIMPLE LITHOPHILS
PROPORTION OF TOLERANT SPECIES
NUMBER OF SENSITIVE SPECIES
PROPORTION OF PIONEER SPECIES
. NUMBER OF INDIVIDUALS/METER
. PERCENT DELT
TOTAL IBI SCORE
6
2.3%
0.232
3
90.1%
9.9%
5.8%
97.3
1
91.5%
2.89
0

IBI
SCORE
3
1
1
1
1
1
1
I
1
1
1
5
18

-------
Lake Agassiz Plain

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94-105 Drainage Area: 12 mi2
Site: ND: Cass Co: Tributary to Rush River, 2.5 mi S, .75 mi E Erie.
Long: 97° 22' 21" Lat: 47° 04' 44".
ACTUAL
METRIC (for headwater streams <200 mi2) OBSERVATION
1. TOTAL NUMBER OF SPECIES 4
2. PROPORTION OF HEADWATER SPECIES 45.3%
3. EVENNESS 0.637
4. NUMBER OF MINNOW SPECIES 2
5. PROPORTION OF OMNIVORE BIOMASS 72.7%
6. PROPORTION OF INSECTIVORE BIOMASS 27.3%
7. PROPORTION OF SIMPLE LITHOPHILS 39.4%
8. PROPORTION OF TOLERANT SPECIES 60.8%
9. NUMBER OF SENSITIVE SPECIES 1
10. PROPORTION OF PIONEER SPECIES 54.5%
11. NUMBER OF INDIVIDUALS/METER 8.22
12. PERCENT DELT 0
TOTAL IBI SCORE
Date: 9-09- 1994
IB!
SCORE
5
3
3
5
1
1
3
3
5
3
5
5
42

-------
                                                  Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-106               Drainage Area: 58 mi2

Site: ND: Trail Co: Elm River, 2 mi E, .5 mi S Galesburg.
Long: 97° 21'56" Lat: 47° 15'39".
                                                                     Date: 9-09-1994
METRIC (for headwater streams <200 mi2)
                                                 ACTUAL
                                                 OBSERVATION
IBI
SCORE
MOD.
1.  TOTAL NUMBER OF SPECIES                            2                 1

2.  PROPORTION OF HEADWATER SPECIES                  8.4%              1

3.  EVENNESS                                          0.416              1

4.  NUMBER OF MINNOW SPECIES                          1                  1

5.  PROPORTION OF OMN1VORE BIOMASS                   94.5%             1

6.  PROPORTION OF INSECTIVORE BIOMASS                 5.5%              1

7.  PROPORTION OF SIMPLE LITHOPHILS                    0                  1

8.  PROPORTION OF TOLERANT SPECIES                    1%                1

9.  NUMBER OF SENSITIVE SPECIES                        0                  1

10. PROPORTION OF PIONEER SPECIES                      91.6%             1

11. NUMBER OF INDIVIDUALS/METER                      2164              5

12. PERCENT DELT                                      0                  5

                                    TOTAL IBI SCORE                      20

-------

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94-107 Drainage Area: 106 mi2
Site: ND: Trail Co: Elm River 2.5 mi S, 4 mi E, .2 mi S Blanchard.
Long: 97° 08' 01" Lat: 47° 18' 25".
ACTUAL
METRIC (for headwater streams <200 mi2) OBSERVATION
1. TOTAL NUMBER OF SPECIES 6
2. PROPORTION OF HEADWATER SPECIES 1.4%
3. EVENNESS 0.629
4. NUMBER OF MINNOW SPECIES 1
5. PROPORTION OF OMNIVORE BIOMASS 99.3%
6. PROPORTION OF INSECTIVORE BIOMASS 0.7%
7. PROPORTION OF SIMPLE LITHOPHILS 9.6%
8. PROPORTION OF TOLERANT SPECIES 97.3
9. NUMBER OF SENSITIVE SPECIES 0
10. PROPORTION OF PIONEER SPECIES 42.5%
11. NUMBER OF INDIVIDUALS/METER 0.73
12. PERCENT DELT 0
TOTAL IBI SCORE
Date: 9-09- 1994
IBI
SCORE
3
1
3
1
1
1
1
1
1
3
1
5
22

-------
                                                       Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-108                 Drainage Area: 126 mi2                     Date: 9-09-1994

Site: ND: Trail Co: North Branch Elm River, 1 mi S, 2 mi E Kelso.
Long: 96° 59' 18" Lat: 47° 18'40".
ACTUAL IBI
METRIC (for headwater streams <200 mi2) OBSERVATION SCORE
MOD.
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11
12.

TOTAL NUMBER OF SPECIES
PROPORTION OF HEADWATER SPECIES
EVENNESS
NUMBER OF MINNOW SPECIES
PROPORTION OF OMNIVORE BIOMASS
PROPORTION OF INSECTIVORE BIOMASS
PROPORTION OF SIMPLE LITHOPHILS
PROPORTION OF TOLERANT SPECIES
NUMBER OF SENSITIVE SPECIES
PROPORTION OF PIONEER SPECIES
. NUMBER OF INDIVIDUALS/METER
. PERCENT DELT
TOTAL IBI SCORE
2 1
78% 5
0.76 3
1 1
48.4% 1
5 1 .6% 1
0 1
1% 1
0 1
22% 1
1.73 1
0 5
22*

-------
Lake Aeassiz Plain

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94- 1 09 Drainage Area:
Site: ND: Trail Co: Goose River, 4 mi E, 1.4 mi N Hillsboro.
Long: 96° 57' 49" Lat: 47° 25' 51".
METRIC (for moderate size streams 200-1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT

1203 mi2

ACTUAL
OBSERVATION
11
6
0.813
4
22.9%
0.2%
76.9%
15%
47.5%
5
0.27
0

Date: 9- 10- 1994

IBI
SCORE
3
3
5
3
5
5
5
1
3
3
1
5
                                     TOTAL IBI SCORE                     42

-------
                                                        Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-110                 Drainage Area: 180 mi:                     Date: 9-10-1994

Site: ND: Trail Co: North Branch Goose River,  2 mi S, 5.5 mi E Mayville.
Long: 97° 12' 38" Lat: 47° 28' 09".
ACTUAL
METRIC (for headwater streams <200 mi2) OBSERVATION
1.
2.
*>
j.
4.
5.
6.
7.
8.
9.
10.
11.
12.

TOTAL NUMBER OF SPECIES
PROPORTION OF HEADWATER SPECIES
EVENNESS
NUMBER OF MINNOW SPECIES
PROPORTION OF OMNIVORE BIOMASS
PROPORTION OF INSECTIVORE BIOMASS
PROPORTION OF SIMPLE LITHOPHILS
PROPORTION OF TOLERANT SPECIES
NUMBER OF SENSITIVE SPECIES
PROPORTION OF PIONEER SPECIES
NUMBER OF INDIVIDUALS/METER
PERCENT DELT
TOTAL IBI SCORE
9
4.1%
0.43
3
25.3%
74.7%
2.5%
88.6%
0
91.8%
6.32
0

IBI
SCORE
3
1
1
1
5
5
1
1
1
1
->
j
5
28

-------
   Agassiz Plain
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-111                 Drainage Area: 478 mi2

Site: ND: Trail Co: Goose River, 6 mi S, .5 mi W Hatton.
Long: 97° 27' 33" Lat: 47° 32' 30".
Date: 9-10-1994
ACTUAL IBI
METRIC (for moderate size streams 200- 1 500 mi2) OBSERVATION SCORE
MOD.
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12

TOTAL NUMBER OF SPECIES
NUMBER OF BENTHIC INSECTIVORES
EVENNESS
NUMBER OF MINNOW SPECIES
PROPORTION OF PISCIVORE BIOMASS
PROPORTION OF OMNIVORE BIOMASS
PROPORTION OF INSECTIVORE BIOMASS
PROPORTION OF SIMPLE LITHOPHILS
PROPORTION OF TOLERANT SPECIES
NUMBER OF SENSITIVE SPECIES
NUMBER OF INDIVIDUALS/METER
PERCENT DELT
TOTAL IBI SCORE
6 1
1 1
0.868 5
3 1
49.2% 1
49.1% 1
1 .7% 1
36.4% 1
63.6% 1
0 1
0.22 1
4.5 1
16*

-------
                                                       Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-112                 Drainage Area: 180 mi2                     Date: 9-14-1994

Site: ND: Steele Co: Middle Branch Goose River, 8 mi W on Hwy 200, 1 mi S, 1 mi W Mayville.
Long: 97° 32' 16" Lat: 47° 30' 45".
METRIC (for headwater streams <200 mi2)
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL
ACTUAL
OBSERVATION
9
30%
0.664
4
53.1%
46.9%
35.4%
47 3%
0
17.3%
1.73
0
IBI SCORE
IBI
SCORE
-i
_>
3
i
j
3
3
i
j
3
3
1
5
1
5
36

-------
Lake Agassiz Plain

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94-1 13 Drainage Area: 160 mi2
Site: ND: Steele Co: Beaver Creek at USGS Gauge.
Long: 97° 42' 27" Lat: 47° 35' 37".
ACTUAL
METRIC (for headwater streams <200 mi2) OBSERVATION
1. TOTAL NUMBER OF SPECIES 4
2. PROPORTION OF HEADWATER SPECIES 2.7%
3. EVENNESS 0705
4. NUMBER OF MINNOW SPECIES 2
5. PROPORTION OF OMNIVORE BIOMASS 62.8%
6. PROPORTION OF INSECTIVORE BIOMASS 37.2%
7. PROPORTION OF SIMPLE LITHOPHILS 17.5%
8. PROPORTION OF TOLERANT SPECIES 1%
9. NUMBER OF SENSITIVE SPECIES 0
10. PROPORTION OF PIONEER SPECIES 79.8%
11. NUMBER OF INDIVIDUALS/METER 7.42
12. PERCENT DELT v 1.3%
TOTAL IBI SCORE

Date: 9- 10- 1994

IBI
SCORE
MOD.
1
1
3
1
1
1
1
1
1
1
5
3
20*

-------
                                                        Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-114                Drainage Area: 2200 mi2                    Date: 9-07-1994

Site: ND. Cass Co: Wild Rice River, 2.5 mi S, .3 mi W St. Benedict.
Long: 96° 50' 49" Lat: 46° 41' 17".
ACTUAL IBI
METRIC (for large streams >1 500 mi2) OBSERVATION SCORE
MOD.
1.
2.
3.
4.
5.
6.
7.
8.
9.
10
11
12

TOTAL NUMBER OF SPECIES
PROPORTION OF LARGE RIVER SPECIES
PROPORTION OF ROUND BODIED SUCKERS
EVENNESS
PROPORTION OF P1SCIVORE BIOMASS
PROPORTION OF OMNIVORE BIOMASS
PROPORTION OF INSECTIVORE BIOMASS
PROPORTION OF SIMPLE LITHOPHILS
PROPORTION OF TOLERANT SPECIES
. NUMBER OF SENSITIVE SPECIES
. NUMBER OF INDIVIDUALS/METER
. PERCENT DELT
TOTAL IBI SCORE
8 1
5.5% 1
1.8% 1
0.807 5
5.2% 1
53.4% 1
41.4% 1
5.5% 1
63.6% 1
1 1
0.55 1
0 5
20*

-------
Lake Aeassiz Plain

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94-1 15 Drainage Area
Site: ND: Richland Co: Wild Rice River, 5.5 mi S, .4 mi W
Long: 96° 44' 29" Lat: 46° 22' 11".
METRIC (for large streams > 1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF LARGE RIVER SPECIES
3. PROPORTION OF ROUND BODIED SUCKERS
4. EVENNESS
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES

: 2000 mi2 Date: 9-07-1994
Abercrombie.
ACTUAL IB!
OBSERVATION SCORE
9 3
0 1
3.7% 1
0.799 5
11.5% 3
53.9% 3
34.6% 3
14.8% 1
22.2% 5
  10. NUMBER OF SENSITIVE SPECIES                       1                 1




  11. NUMBER OF INDIVIDUALS/METER                      0.27              1




  12. PERCENT DELT                                     0                 5




                                     TOTAL IBI SCORE                     32

-------
                                                    Minnesota-North Dakota Ecoregion
 LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

 Station Number: 94-116                Drainage Area: 1297 mi2                  Date: 9-07-1994

 Site: Co: Richland Co: Wild Rice River, 3.5 mi N Hankinson.
 Long: 96° 54' 24" Lat: 46° 07' 52".	
                                                   ACTUAL              IBI
 METRIC (for moderate size streams 200-1500 mi2)             OBSERVATION         SCORE
	MOD.	

 1.  TOTAL NUMBER OF SPECIES                            6                 1

 2.  NUMBER OF BENTHIC INSECTIVORES                     1                 1

 3.  EVENNESS                                           0.456              1

 4.  NUMBER OF MINNOW SPECIES                          1                 1

 5.  PROPORTION OF PISCIVORE BIOMASS                    83.4%              1

 6.  PROPORTION OF OMNIVORE BIOMASS                    15.2%              1

 7.  PROPORTION OF INSECTIVORE BIOMASS                  1.4%              1

 8.  PROPORTION OF SIMPLE LITHOPHILS                     4.3%              1

 9.  PROPORTION OF TOLERANT SPECIES                     87.1%              1

 10. NUMBER OF SENSITIVE SPECIES                         0                 1

 11. NUMBER OF INDIVIDUALS/METER                      0.93               1

 12. PERCENT DELT                                       0                 5

                                     TOTAL IBI SCORE                      16*

-------
Lake Agassiz Plain

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94-1 17 Drainage

Area: 215 mi2 Date: 9-07-1994
Site: Co: Richland Co: Antelope Creek, 5.5 mi S, .8 mi W Abercrombie.
Long: 96° 45' 08" Lat: 46° 22' H".
METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
ACTUAL IBI
OBSERVATION SCORE
6 3
2 1
0.46 1
2 1
0 1
96.8% 1
3.2% 1
0.4% 1
98.3% 1
0 1
5.34 3
0 5
                                    TOTAL IBI SCORE                     20

-------
                                                        Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-118                Drainage Area: 980 mi2                     Date: 9-07-1994

Site:  Co: Richland Co:  Bois de Sioux, 1.5 mi E Fairmount.
Long: 96° 33' 58" Lat: 46° 03'03".
ACTUAL
METRIC (for moderate size streams 200-1500 mi2) OBSERVATION
1.
2.
->
j.
4.
5.
6.
7.
8.
9.
10.
11
12,

TOTAL NUMBER OF SPECIES
NUMBER OF BENTHIC INSECTIVORES
EVENNESS
NUMBER OF MINNOW SPECIES
PROPORTION OF PISCIVORE BIOMASS
PROPORTION OF OMNIVORE BIOMASS
PROPORTION OF INSECTIVORE BIOMASS
PROPORTION OF SIMPLE LITHOPHILS
PROPORTION OF TOLERANT SPECIES
. NUMBER OF SENSITIVE SPECIES
. NUMBER OF INDIVIDUALS/METER
. PERCENT DELT
TOTAL IBI SCORE
9
3
0.654
3
29.4%
3.1%
64.2%
1.1%
51.1%
1
0.61
0

IBI
SCORE
i
J
1
->
j
1
5
5
5
1
3
1
1
5
34

-------

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94- 1 1 9 Drainage Area:
Site: Co: Richland Co: Red River at Brushvale.
Long: 96° 39' 24" Lat: 46° 22' 11".
METRIC (for large streams >1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF LARGE RIVER SPECIES
3. PROPORTION OF ROUND BODIED SUCKERS
4. EVENNESS
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI

4200 mi2

ACTUAL
OBSERVATION
15
29.9%
2.5%
0.474
3 1 .4%
21.9%
46.6%
5.1%
3.2%
6
1.05
1.9
SCORE

Date: 9-07- 1994

IBI
SCORE
3
3
1
1
3
5
3
1
5
3
1
3
32

-------
                                                       Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-120                Drainage Area: 51 mi2                      Date: 9-08-1994

Site: ND: Cass Co: Swan Creek, 2 mi N, 4 mi W, .5 mi N Casselton.
Long: 97° 18' 09" Lat: 46° 56' 17".
ACTUAL
METRIC (for headwater streams <200 mi2) OBSERVATION
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.

TOTAL NUMBER OF SPECIES
PROPORTION OF HEADWATER SPECIES
EVENNESS
NUMBER OF MINNOW SPECIES
PROPORTION OF OMNIVORE BIOMASS
PROPORTION OF INSECTIVORE BIOMASS
PROPORTION OF SIMPLE LITHOPHILS
PROPORTION OF TOLERANT SPECIES
NUMBER OF SENSITIVE SPECIES
PROPORTION OF PIONEER SPECIES
NUMBER OF INDIVIDUALS/METER
PERCENT DELT
TOTAL IB! SCORE
9
10.2%
0.506
5
51.3%
48.5%
3.7%
91.4%
0
75.9%
3.74
0

IBI
SCORE
3
1
1
5
->
j
-t
j
1
1
1
1
1
5
26

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LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94- 1 2 1 Drainage Area:
Site: ND: Cass Co: Maple River, 1 mi S, 3 mi W Mapleton.
Long: 97 ° 06' 59" Lat: 46 ° 5 11 4 1".
METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT

1275 mi2

ACTUAL
OBSERVATION
8
2
0.393
3
33.2%
28.6%
38.2%
1 .5%
26.1%
0
1.33
0

Date: 9-08- 1994

IBI
SCORE
3
1
1
1
3
5
3
1
5
1
1
5
TOTAL IBI SCORE                    30

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                                                  Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-122                Drainage Area:  1022 mi2                   Date: 9-08-1994

Site: ND: Cass Co: Maple River, .5 mi W, 3 mi S, .5 mi W, 1.5 mi S Chaffee.
Long: 97° 22' 45" Lat: 46° 42' 39".	
                                                  ACTUAL             IB!
METRIC (for moderate size streams 200-1500 mi2)	OBSERVATION        SCORE

1.  TOTAL NUMBER OF SPECIES                            10                3

2.  NUMBER OF BENTHIC INSECT1VORES                     4                 3

3.  EVENNESS                                           0.835              5

4.  NUMBER OF MINNOW SPECIES                           4                 3

5.  PROPORTION OF PISCIVORE BIOMASS                    37%               3

6.  PROPORTION OF OMNIVORE BIOMASS                    30%               5

1.  PROPORTION OF INSECTIVORE BIOMASS                  33%               3

8.  PROPORTION OF SIMPLE LITHOPHILS                     31.1%             3

9.  PROPORTION OF TOLERANT SPECIES                      56.3%             3

10. NUMBER OF SENSITIVE SPECIES                         1                 1

11. NUMBER OF INDIVIDUALS/METER                       1.03               1

12. PERCENT DELT                                       0                 5

                                    TOTAL IBI SCORE                      38

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Lake Agassiz Plain
   LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

   Station Number: 94-123                 Drainage Area: 594 mi2                      Date: 9-08-1994

   Site: ND: Cass Co: Maple River, 1 mi N, 2.6 mi W Alice.
   Long: 97° 36' 40" Lat: 46° 46' 29".
ACTUAL
METRIC (for moderate size streams 200-1500 mi2) OBSERVATION
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.

TOTAL NUMBER OF SPECIES
NUMBER OF BENTHIC INSECTIVORES
EVENNESS
NUMBER OF MINNOW SPECIES
PROPORTION OF PISCIVORE BIOMASS
PROPORTION OF OMNIVORE BIOMASS
PROPORTION OF INSECTIVORE BIOMASS
PROPORTION OF SIMPLE LITHOPHILS
PROPORTION OF TOLERANT SPECIES
NUMBER OF SENSITIVE SPECIES
NUMBER OF INDIVIDUALS/METER
PERCENT DELT
TOTAL IBI SCORE
9
4
0.849
3
25.8%
51.3%
22.9%
23.5%
41.7%
0
2.3
0.4

IBI
SCORE
3
3
5
1
5
3
1
1
3
1
1
5
32

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                                                   Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-124                Drainage Area: 69 mi2                    Date: 9-08-1994

Site: ND: Steele Co: Maple River, 3.5 mi W, .5 mi N Colgate.
Long: 97° 43' 38" Lat: 47° 14' 47".

                                                  ACTUAL             1BI
METRIC (for headwater streams <200 mi:)	OBSERVATION        SCORE

1.  TOTAL NUMBER OF SPECIES                            7                3

2.  PROPORTION OF HEADWATER SPECIES                   10.7%             1

3.  EVENNESS                                           0.375             1

4.  NUMBER OF MINNOW SPECIES                           2                1

5.  PROPORTION OF OMNIVORE BIOMASS                    28.6%             5

6.  PROPORTION OF INSECTIVORE BIOMASS                  4.3%             1

7.  PROPORTION OF SIMPLE LITHOPHILS                     0.5%             1

8.  PROPORTION OF TOLERANT SPECIES                     96.9%             1

9.  NUMBER OF SENSITIVE SPECIES                         0                1

10. PROPORTION OF PIONEER SPECIES                      80.4%             1

11. NUMBER OF INDIVIDUALS/METER                      11.76             5

12. PERCENT DELT                                      0                5

                                   TOTAL IBI SCORE                      26

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Lake Agassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 94-125                 Drainage Area: 148 mi2                     Date: 7-27-1994

  Site: ND: Grand Forks Co: North Branch Turtle River, SH 18 bridge, 3 mi N of Hwy 2 Junct.
  Long: 97° 37'25" Lat: 47° 59' 31".
ACTUAL
METRIC (for headwater streams <200 mi2) OBSERVATION
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.

TOTAL NUMBER OF SPECIES
PROPORTION OF HEADWATER SPECIES
EVENNESS
NUMBER OF MINNOW SPECIES
PROPORTION OF OMNIVORE BIOMASS
PROPORTION OF INSECTIVORE BIOMASS
PROPORTION OF SIMPLE LITHOPHILS
PROPORTION OF TOLERANT SPECIES
NUMBER OF SENSITIVE SPECIES
PROPORTION OF PIONEER SPECIES
NUMBER OF INDIVIDUALS/METER
PERCENT DELT
TOTAL IBI SCORE
10
44.6%
0.54
6
24.6%
75.4%
48.6%
55.2%
1
10.8%
10.84
0.1

IBI
SCORE
5
3
1
3
5
5
3
3
1
5
5
5
44

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                                                        Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-126                 Drainage Area: 148 mi2                     Date: 7-28-1994

Site: ND: Grand Forks Co: North Branch Turtle River, SH 18 bridge, 3 mi N of Hwy 2 Junct.
Long: 97° 37' 25" Lat: 47° 59' 31".
ACTUAL
METRIC (for headwater streams <200 mi2) OBSERVATION
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12

TOTAL NUMBER OF SPECIES
PROPORTION OF HEADWATER SPECIES
EVENNESS
NUMBER OF MINNOW SPECIES
PROPORTION OF OMNIVORE BIOMASS
PROPORTION OF INSECTIVORE BIOMASS
PROPORTION OF SIMPLE LITHOPHILS
PROPORTION OF TOLERANT SPECIES
NUMBER OF SENSITIVE SPECIES
PROPORTION OF PIONEER SPECIES
. NUMBER OF INDIVIDUALS/METER
. PERCENT DELT
TOTAL IBI SCORE
9
48.4%
0.604
5
21.6%
78%
50.9%
62.1%
0
16.2%
7
0.4

IBI
SCORE
3
3
•>
j
3
5
5
•>
3
1
1
5
••>
j
5
40

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Lake Agassiz Plain

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94-127 Drainage
Site: ND: Pembina Co: Tongue River, SH 18, 3.25 mi
Long: 97° 33' 22" Lat: 48° 51' 06".
METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT

Area: 295 mi2 Date: 7-25-1994
SW Bathgate.
ACTUAL IBI
OBSERVATION SCORE
7 3
2 1
0.851 5
3 1
0 1
34.9% 3
65.1% 5
46% 3
51.8% 3
0 1
1.39 1
13.7% 1
                                     TOTAL IBI SCORE                      28

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              Minnesota-North Dakota Ecoregion

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94-128 Drainage
Site: ND: Pembina Co: Pembina River, SH 32 bridge,
Long: 97° 55' 01" Lat: 48° 54' 49".
METRIC (for large streams > 1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF LARGE RIVER SPECIES
3. PROPORTION OF ROUND BODIED SUCKERS
4. EVENNESS
5. PROPORTION OF P1SCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT

Area: 3200 mi2
0.75 mi S Walhalla.
ACTUAL
OBSERVATION
14
0
0
0.712
12.8%
12.4%
70.6%
19.4%
34.8%
5
1.4
0

Date: 7-25-1994

IBI
SCORE
3
1
1
3
3
5
5
1
3
3
1
5
TOTAL IBI SCORE                      34

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Lake Agassiz Plain
  LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

  Station Number: 94-129                 Drainage Area: 66 mi2                      Date: 7-26-1994

  Site: ND: Pembina Co: North Branch Park River, 6 mi N Edinburg.
  Long: 97° 51' 42" Lat: 48° 35' 26".
ACTUAL
METRIC (for headwater streams <200 mi2) OBSERVATION
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI SCORE
5
23.5%
0.756
3
10.6%
32.3%
20.8%
98.6%
0
75.2%
6.33
0.5%

IBI
SCORE
^
j
1
3
3
5
•j
j
1
1
1
1
3
5
30

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                                                        Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-130                Drainage Area: 416 mi2                      Date: 7-27-1994

Site: ND: Grand Forks Co: Forest River, 2 mi N Inkster.
Long: 97° 38' 37" Lat: 48° 10' 46".
ACTUAL
METRIC (for moderate size streams 200- 1 500 mi2) OBSERVATION
1.
2.
">
J.
4.
5.
6.
7.
8.
9.
10.
11.
12.

TOTAL NUMBER OF SPECIES
NUMBER OF BENTHIC INSECTIVORES
EVENNESS
NUMBER OF MINNOW SPECIES
PROPORTION OF P1SC1VORE BIOMASS
PROPORTION OF OMNIVORE BIOMASS
PROPORTION OF INSECTIVORE BIOMASS
PROPORTION OF SIMPLE LITHOPHILS
PROPORTION OF TOLERANT SPECIES
NUMBER OF SENSITIVE SPECIES
. NUMBER OF INDIVIDUALS/METER
. PERCENT DELT
TOTAL IBI SCORE
13
6
0.706
8
8.5%
6.5%
77.6%
37.7%
43.8%
3
9.02
0.1%

IBI
SCORE
3
3
3
5
1
5
5
3
3
3
5
5
44

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Lake Agassiz Plain

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94- 1 3 1 Drainage Area:

180 mi2

Date: 9- 14- 1994
Site: ND: Trail Co: North Branch Goose River, 2 mi S, 5.5 mi E Mayville.
Long: 97° 12' 38" Lat: 47° 28' 09".
METRIC (for headwater streams <200 mi2)
1. TOTAL NUMBER OF SPECIES
2. PROPORTION OF HEADWATER SPECIES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF OMNIVORE BIOMASS
6. PROPORTION OF INSECTIVORE BIOMASS
7. PROPORTION OF SIMPLE LITHOPHILS
8. PROPORTION OF TOLERANT SPECIES
9. NUMBER OF SENSITIVE SPECIES
10. PROPORTION OF PIONEER SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI
ACTUAL
OBSERVATION
9
6.5%
0.46
4
34.3%
65.7%
0.8%
80%
0
85.8%
5.2
0
SCORE
IBI
SCORE
3
1
1
3
3
5
1
1
1
1
3
5
28

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                                                         Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-502                  Drainage Area: 254 mi2                      Date: 8-09-1994

Site: ND: Grand Forks Co: Turtle River at State Park, reach # 2.
Long: 97° 30' 00" Lat: 47° 56' 18".
ACTUAL
METRIC (for moderate size streams 200-1500 mi2) OBSERVATION
1.
2.
••>
4.
5.
6.
7.
8.
9.
10.
11.
12.

TOTAL NUMBER OF SPECIES
NUMBER OF BENTHIC INSECTIVORES
EVENNESS
NUMBER OF MINNOW SPECIES
PROPORTION OF PISCIVORE BIOMASS
PROPORTION OF OMNIVORE BIOMASS
PROPORTION OF INSECTIVORE BIOMASS
PROPORTION OF SIMPLE LITHOPHILS
PROPORTION OF TOLERANT SPECIES
NUMBER OF SENSITIVE SPECIES
NUMBER OF INDIVIDUALS/METER
PERCENT DELT
TOTAL IBI SCORE
12
7
0.731
7
0
17.9%
82.1%
18.2%
53.5%
2
5.46
3.8%

IBI
SCORE
5
5
->
j
5
1
5
5
1
3
3
•>
j
3
42

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LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-503 Drainage Area: 405 mi2
Site: ND: Grand Forks Co: Forest River near Fordville.
Long: 97° 43' 49" Lat: 48° 1 1' 50".
METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL

ACTUAL
OBSERVATION
16
6
0.687
9
12.9%
32.6%
54.2%
14.4%
40.7%
3
20.73
0.7%
IBI SCORE

Date: 8-18-1994

IBI
SCORE
5
3
3
5
3
3
3
1
3
3
5
5
42

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                                                        Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-504                 Drainage Area: 1240 mi2                      Date: 8-30-1994

Site: ND: Trail Co: Goose River at Hillsboro.
Long: 97° 03' 39" Lat: 47° 24' 34".
ACTUAL
METRIC (for moderate size streams 200- 1 500 mi:) OBSERVATION
1.
2.
'j
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.

TOTAL NUMBER OF SPECIES
NUMBER OF BENTHIC INSECTIVORES
EVENNESS
NUMBER OF MINNOW SPECIES
PROPORTION OF PISC1VORE BIOMASS
PROPORTION OF OMNIVORE BIOMASS
PROPORTION OF INSECTIVORE BIOMASS
PROPORTION OF SIMPLE LITHOPHILS
PROPORTION OF TOLERANT SPECIES
NUMBER OF SENSITIVE SPECIES
NUMBER OF INDIVIDUALS/METER
PERCENT DELT
TOTAL IB1 SCORE
20
5
0.645
7
37.8%
31.6%
30.6%
28.3%
29%
3
0.97
0

IBI
SCORE
5
3
3
5
3
5
3
1
5
3
1
5
42

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LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-505 Drainage Area: 3202 mi2
Site: ND: Pembina Co: Pembina River at Walhalla.
Long: 97° 55' 00" Lat: 48° 54' 50".
METRIC (for large streams > 1 500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF LARGE RIVER SPECIES
3. PROPORTION OF ROUND BODIED SUCKERS
4. EVENNESS
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL

ACTUAL
OBSERVATION
19
0
0.3%
0.631
31%
2.3%
64.2%
68.1%
36.6%
6
6.06
0.4%
IBI SCORE

Date: 8- 17- 1994

IBI
SCORE
5
1
1
3
3
5
5
1
3
3
5
5
40

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Minnesota-North Dakota Ecoregion

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94-523 Drainage Area
Site: ND: Grand Forks Co: Turtle River at State Park, reach
Long: 97° 30' 00" Lat: 47° 56' 18".
METRIC (for moderate size streams 200-1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT

: 254 mi2
# 1.
ACTUAL
OBSERVATION
16
7
0.75
7
6.4%
22.7%
62.8%
19.6%
57.8%
3
3.02
2%
TOTAL IBI SCORE

Date: 8-09-1994

IBI
SCORE
5
5
3
5
1
5
5
1
3
3
1
3
40

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Lake Aeassiz Plain

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94-524 Drainage Area
Site: ND: Grand Forks Co: Turtle River at State Park, reach
Long: 97° 30' 00" Lat: 47° 56' 18".
METRIC (for moderate size streams 200-1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT

: 254 mi2
#3.
ACTUAL
OBSERVATION
12
7
0.805
7
0
12.3%
81.3%
30.7%
57.8%
3
3.02
2%
TOTAL IBI SCORE

Date: 8-10-1994

IBI
SCORE
5
5
5
5
1
5
5
3
3
3
1
3
44

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                                                        Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-525                 Drainage Area: 4490 mi2                      Date: 8-25-1994

Site: ND: Ransom Co: Sheyenne River at Lisbon, reach # 2.
Long: 97° 40' 44" Lat: 46° 26' 49".
ACTUAL
METRIC (for large streams > 1500 mi2) OBSERVATION
1.
2.
o
j.
4.
5.
6.
7.
8.
9.
10.
11.
12.

TOTAL NUMBER OF SPECIES
PROPORTION OF LARGE RIVER SPECIES
PROPORTION OF ROUND BODIED SUCKERS
EVENNESS
PROPORTION OF PISCIVORE BIOMASS
PROPORTION OF OMNIVORE BIOMASS
PROPORTION OF INSECTIVORE BIOMASS
PROPORTION OF SIMPLE LITHOPHILS
PROPORTION OF TOLERANT SPECIES
NUMBER OF SENSITIVE SPECIES
NUMBER OF INDIVIDUALS/METER
PERCENT DELT
TOTAL IBI SCORE
23
1.8%
13.1%
0.714
13.4%
45.5%
41.2%
22.9%
21.8%
6
1.83
3.6%

IBI
SCORE
5
1
1
1
j
3
3
3
1
5
3
->
j
3
34

-------

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94-526 Drainage Area: 4490 mi2
Site: ND: Ransom Co: Sheyenne River at Lisbon, reach # 1 .
Long: 97° 40' 44" Lat: 46° 26' 49".
METRIC (for large
streams >1500 mi2)
1. TOTAL NUMBER OF SPECIES
2. PROPORTION
3. PROPORTION
4. EVENNESS
5. PROPORTION
6. PROPORTION
7. PROPORTION
8. PROPORTION
9. PROPORTION
10. NUMBER OF
11. NUMBER OF
OF LARGE RIVER SPECIES
OF ROUND BODIED SUCKERS

OF PISCIVORE BIOMASS
OF OMNIVORE BIOMASS
OF INSECTIVORE BIOMASS
OF SIMPLE LITHOPHILS
OF TOLERANT SPECIES
SENSITIVE SPECIES
INDIVIDUALS/METER
12. PERCENT DELT
ACTUAL
OBSERVATION
22
2.2%
11.2
0.691
24.8%
13.1%
62.1%
19.1%
19.7%
9
1.19
2.8%
Date: 8-25-1994
IBI
SCORE
5
1
1
3
5
5
5
1
5
5
1
3
TOTAL IBI SCORE                    40

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                                                       Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-527                Drainage Area: 1877 mi2                     Date: 8-17-1994

Site: ND: Eddy Co: Sheyenne River S of Warwick.
Long: 98° 42' 57" Lat: 47° 48' 20".
ACTUAL
METRIC (for large streams >1500 mi2) OBSERVATION
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.

TOTAL NUMBER OF SPECIES
PROPORTION OF LARGE RIVER SPECIES
PROPORTION OF ROUND BODIED SUCKERS
EVENNESS
PROPORTION OF PISCIVORE BIOMASS
PROPORTION OF OMNIVORE BIOMASS
PROPORTION OF INSECTIVORE BIOMASS
PROPORTION OF SIMPLE LITHOPHILS
PROPORTION OF TOLERANT SPECIES
NUMBER OF SENSITIVE SPECIES
NUMBER OF INDIVIDUALS/METER
PERCENT DELT
TOTAL IBI SCORE
17
0.9%
0
0.785
9.4%
49.3%
41.3%
26.5%
41.4%
3
12.74
1.2%

IBI
SCORE
5
1
1
5
1
3
3
1
3
1
5
-i
:>
32

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Lake Aeassiz Plain

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94-528 Drainage Area:
Site: ND: Pembina Co: Red River E of Joliette, reach # 2.
Long: 97° 10' 43" Lat: 48° 48' 51".
METRIC (for large streams > 1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF LARGE RIVER SPECIES
3. PROPORTION OF ROUND BODIED SUCKERS
4. EVENNESS
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMN I VORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT

3 1680 mi2

ACTUAL
OBSERVATION
18
58.5%
0
0.787
36.4%
19.9%
43.6%
17.1%
20.3%
5
0.26
0
TOTAL IBI SCORE

Date: 9-02- 1994

IBI
SCORE
5
5
1
5
3
5
3
1
5
3
5
5
46

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LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94-529 Drainage Area:
Site: ND: Pembina Co: Red River E of Joliette, reach # 3.
Long: 97° 10' 43" Lat: 48° 48' 51".
METRIC (for large streams >1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF LARGE RIVER SPECIES
3. PROPORTION OF ROUND BODIED SUCKERS
4. EVENNESS
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT

31680 mi2

ACTUAL
OBSERVATION
16
64.9%
3.1%
0.907
21%:
3.1%
75.8%
37.1%
17.5%
5
0.19
0
TOTAL IB! SCORE

Date: 9-02-1994

IBI
SCORE
3
5
1
5
3
5
5
3
5
3
5
5
48

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Lake Aeassiz Plain

LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94-530 Drainage Area:
Site: ND: Pembina Co: Red River E of Joliette, reach # 1 .
Long: 97° 10' 43" Lat: 48° 48' 51".
METRIC (for large streams > 1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. PROPORTION OF LARGE RIVER SPECIES
3. PROPORTION OF ROUND BODIED SUCKERS
4. EVENNESS
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
1 1 . NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT
TOTAL IBI

31680 mi2

ACTUAL
OBSERVATION
18
46%
1 .4%
0.872
39.8%
9.2%
50.8%
22.3%
13.7%
6
0.28
0
SCORE

Date: 9-02- 1994

IBI
SCORE
5
3
1
5
3
5
3
1
5
3
5
5
44

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                                                        Minnesota-North Dakota Ecoregion
LAKE AGASSIZ PLAIN BIOCRITERIA STUDY

Station Number: 94-531                 Drainage Area: 6690 mi2                     Date: 8-11-1994

Site: ND: Cass Co: Red River at Fargo.
Long: 97° 47' 00" Lat: 46° 51' 40".
ACTUAL
METRIC (for large streams > 1500 mi2) OBSERVATION
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.

TOTAL NUMBER OF SPECIES
PROPORTION OF LARGE RIVER SPECIES
PROPORTION OF ROUND BODIED SUCKERS
EVENNESS
PROPORTION OF PISCIVORE BIOMASS
PROPORTION OF OMNIVORE BIOMASS
PROPORTION OF INSECTIVORE BIOMASS
PROPORTION OF SIMPLE LITHOPHILS
PROPORTION OF TOLERANT SPECIES
NUMBER OF SENSITIVE SPECIES
NUMBER OF INDIVIDUALS/METER
PERCENT DELT
TOTAL IBI SCORE
16
53.4%
0.2%
0.355
9%
66.5%
24.5%
0.5%
2.3%
6
6.61
0

IBI
SCORE
••>
j
5
1
1
1
1
1
1
5
3
5
5
32

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LAKE AGASSIZ PLAIN BIOCRITERIA STUDY
Station Number: 94-533 Drainage Area:
Site: ND: Ransom Co: Maple River at Enderlin.
Long: 97° 34' 25" Lat: 46° 37' 18".

METRIC (for moderate size streams 200-1500 mi2)
1 . TOTAL NUMBER OF SPECIES
2. NUMBER OF BENTHIC INSECTIVORES
3. EVENNESS
4. NUMBER OF MINNOW SPECIES
5. PROPORTION OF PISCIVORE BIOMASS
6. PROPORTION OF OMNIVORE BIOMASS
7. PROPORTION OF INSECTIVORE BIOMASS
8. PROPORTION OF SIMPLE LITHOPHILS
9. PROPORTION OF TOLERANT SPECIES
10. NUMBER OF SENSITIVE SPECIES
11. NUMBER OF INDIVIDUALS/METER
12. PERCENT DELT

828 mi2

ACTUAL
OBSERVATION
12
5
0.538
6
7.9%
10.6%
81.4%
16.5%
29.4%
0
7.98
0.4%

Date: 8-1 1-1994

IBI
SCORE
3
3
1
3
1
5
5
1
5
1
5
5
TOTAL IBI SCORE                     38

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              REPORT DOCUMENTATION  PAGE
                                                       Form Approved
                                                       OMB No. 0704-0188
  Public reoortmg Durden for this collection or inTormalion .s estimated to average i hour oer resoonse including the time lor revfewmq instructions searching existing data sources
  gathering ana maintaining the data neeaea. ana commeting ang reviewing tne collection ot information iena comments reqaramg this burden estimate or 3nv other asoect ot this
  collection or nformation. inducing suggestions tor reducing this Duraen to Washington Heaaauarters services Directorate tor information Ooerations and fteoorts I2'5 Jefferson
  Davis Highway. Suite 1204 Arlington 74 22202-1302 ana to the Office ot Management ana Budget Paoerwor< Reduction Pro|ea(070.4-0188) Washington DC 20503
    AGENCY USE ONLY (Leave Olank)
          2. REPORT DATE

            August 199R
                                                     3. REPORT TYPE AND DATES COVERED
                                                      FINAL
  4. TITLE AND SUBTITLE
    Development  of Index  of Biotic  Integrity Expectations for
    the Lake Agassiz Plain  Ecoregion.
  6. AUTHOR(S)
   Scott Niemela,
   Goldstein,  and
Eric Pearson, Thomas P.
Patricia  A. Bailey
Simon,  Robert M,
                                                5. FUNDING NUMBERS
  7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES)
   U.S.  Environmental Protection Agency
   Watershed and  Non-Point  Source  Branch
   77  West Jackson  Blvd., WW-16J
   Chicago, Illinois  60604
                                                8. PERFORMING ORGANIZATION
                                                  REPORT NUMBER
                                                EPA 905/R-96-005
 9. SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES)
   Minnesota Pollution Control Agency
   North Dakota  Deoartment  of Health
   U.S.  Geological  Survey
                                                10. SPONSORING/MONITORING
                                                  AGENCY REPORT NUMBER
 11. SUPPLEMENTARY NOTES
 12a. DISTRIBUTION / AVAILABILITY STATEMENT
    UNLIMITED
                                                                   12b. DISTRIBUTION CODE
                                                   UNLIMITED
  3. ABSTRACT (Maximum 200 words)
    The  index of  biotic integrity (IBI)  has been  used to evaluate biological quality
    of rivers and streams in  diverse areas of the United States.   The  IBI compares
    characteristics  of lotic  systems,  termed "metrics", that  represents  the structural
    and  functional attributes  of the fish  community  in three  categories:  1) snecies
    richness and  composition,  2) trophic structure,  and 3) fish abundance and  health.
    Comparisons are  made with  lotic systems possessing unaffected or minimally  affected
    communities from ecologically similar  areas.   Modifications of the metrics  have
    been made to account for  regional  or local  characteristics  of fish communities  A
    combined project between  the USGS; USEPA.vRegq'on  V and VII.I;  Minnesota Pollution
    Control  Agency,  Minnesota  DNR;  and the North  Dakota Department of Health, Division
    of Water Quality targeted  the multi-stat* Lqke».Agassiz flptn" (formerly:.the  Red
    River  Valley) ecoregion for  IBI  development "and  assessmeriV'.'AT], of  the'metrics
    have been modified  for application to  this  biologically yblmd (si hie "aTaciation)
    system.   Several  new metrics are proposed  for  various  stream  sizes in  the Lake
    Agassiz  Plain ecoregion. New metrics include  the  evenness component  of diversity,
    trophic  composition  metrics  based on biomass,  a new omnivore  definition,  and as an
                             of subterminal mouth
 4. SUBJECT TERMsalternative r^j-p-j^  the
               cyprinids.

 Biological  integrity,  Biological  assessment,  ecoregions, IBI
                           15. NUMBER OF PAGES

                              fifl + annpndi ri p«;
                                                     16. PRICE CODE
 7.  SECURITY CLASSIFICATION
    OF REPORT
    UNCLASSIFIED
     18.  SECURITY CLASSIFICATION
        OF THIS PAGE
        UNCLASSIFIED
  19  SECURITY CLASSIFICATION
      OF ABSTRACT

      UNCLASSIFIED
20. LIMITATION OF ABSTRACT

   SAR
NSN 7540-01-280-5500
                                                                       Standard Form 298 (Rev 2 89)
                                                                       P'escnoea ov ANSI std 239-18

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