United States
         Environmental Protection
         Agency
             Region 5
             77 West Jackson Boulevard
             Chicago, Illinois 60604
EPA 905-R-96-007
NTS
September 1997
&EPA
Biological Characterization
of the Middle Fork
Anderson River,
Perry County, Indiana

-------
                                                   EPA Technical Report
BIOLOGICAL CHARACTERIZATION OF THE
MIDDLE FORK ANDERSON RIVER,
PERRY COUNTY, INDIANA

by Thomas P. Simon

-------
                                              CONTENTS
                                                                                                    Page
Abstract	               1
Introduction	              1
        Purpose and Scope	            2
        Description  of the Study Area	         2
Methods   	              3
        Survey Design	             3
        Sample  Collection	            3
Results	                 7
        Biological Integrity  	             7
        Designated  Uses	            10
References  	               10
                                           ILLUSTRATIONS
Figures
        1. Project  Map showing the location of sampling locations on the Middle Fork Anderson River .  . .4

        2. Trends  in biotic integrity for the Middle Fork Anderson River	8

        3. Trends  in biotic integrity for Sulphur Fork, Kraus Creek, and  Theis Creek	    8

                                                TABLES

Table   1.      Interim Scoring Criteria for 12 IBI metrics for low to moderate gradient
                stream systems within the Interior River Lowland for the Ohio River
                subsection  	               6

        2.      Biotic Integrity classes used in assessing fish communities along with
                general descriptions of their attributes  (Karr et al. 1986)	               7

        3.      Habitat quality of 12  locations in the Middle Fork Anderson  River watershed	9

                                              APPENDIX

        1.      Metric scoring for individual stations sampled within the Middle Fork Anderson River. . 12.

        2.      Statewide list of Indiana species considered to be headwater  species for
                evaluating  permanent  habitat in headwater streams  (after Smith 1971). . .42

        3.      Statewide list of Indiana fish species considered  to be sensitive to a wide
                variety of environmental disturbances including water quality and habitat
                degradation.                                                                           43

        4.      Statewide list of species considered to  be highly tolerant  to a wide variety
                of environmental disturbances including water quality and habitat
                degradation.                                                                           44

        5.      Statewide  list of Indiana fish species considered  to be omnivores.                         45

        6.       Statewide  list of Indiana fish species considered  to be msectivores.                        46

-------
Biological Characterization
                                      APPENDIX (CONTINUED)

        7.       Statewide  list of Indiana fish species considered to be carnivores.                         48

        8.       Statewide  list of Indiana species considered to be pioneer species
                (indicators of temporally  unavailable  or stressed  habitats (after Larimore
                and Smith  1963; Smith 1971)).                                                         49

        9.       Statewide  list of Indiana fish species considered to be simple lithophilous
                spawners.                                                                             50

-------
                                                                            Middle Fork Anderson River
BIOLOGICALCHARACTERIZATIONOF THE MIDDLE FORK ANDERSON RIVER,
PERRY COUNTY, INDIANA

by Thomas P. Simon

Abstract

The  Middle Fork of the Anderson River is currently under review for the completion of an intensive project
channelizing a significant portion of the watershed.  During the fall of 1994, a biological  characterization
study evaluated the integrity of twelve locations on the Middle Fork Anderson River and tributaries.  The
biological conditions found in the watershed reflected complete recovery from an earlier  channelization event
and was habitat limited only in areas  which has been routinely maintained through select dredging projects.
Fish community attributes reflected good IBI scores  for portions of the watershed  which would be scheduled
for rechannelization, suggesting that the  biological communities are typical of similar streams with general
designated use category. A use attainability analysis for the lower Middle Fork Anderson River should be
conducted  to preserve  this reach as an exceptional aquatic  resource.

Introduction

The  Middle Anderson  River  has been selected  as a watershed within Indiana which is considered for flow
modification under the authority of the  Natural Resources  Conservation Service (NRCS).  This program is
designed to mitigate flooding  and ecosystem decline  through habitat modification including channelization
and  flood control structure construction. The Middle Fork Anderson River has been a targeted watershed
due  to the potential high instantaneous  flow measured  in the system and the associated impacts on
agriculture and adjacent land  use.  The Middle Fork contains about 170 miles of permanent and  intermittent
streams of which 34 miles have been planned for channel work as part  of the Middle Fork Anderson river
project under the provision of PL 566.  The work plan for the Middle Fork was authorized  on August 28,
 1961. As of 1980,11.9 miles  of stream  channelization  has  been completed (Hottell 1980). Flood control
 structure, channelization,  shoal  removal, clearing and snagging are  recommended for the Middle Fork
 Anderson  River in order  to facilitate flood  damage control, sediment overwash and swamping, erosion
 damage, water management  problems with drainage including excavation of drainage channels.  The primary
 reason for the project includes  reduction of total land  acreage subject to flooding (NRCS 1980).

 During the fall of 1994, the U.S. Environmental Protection Agency (USEPA) and Indiana  Department  of
 Environmental Management   (IDEM) evaluated the  biological potential of the Middle Fork Anderson River
 to determine  the potential for meeting  current designated  uses.  The biological structure and function was
 evaluated  using the Index of  Biotic Integrity, a multi-metric index used to quantify water resource integrity
 (Karr 1981;Karr et al. 1986).

 The term biological integrity originated  in  the  Water Pollution Control Act Amendments of 1972 (PL 92-
 500). Karr and Dudley (1981) defined biological integrity as, "the ability of an aquatic ecosystem  to support
 and  maintain a balanced, integrated, adaptive community  of organisms having a species  composition,
 diversity, and functional organization comparable  to the best natural  habitats within a region". Expectations
 for the regional areas of similarity in Indiana,  termed  "reference condition", have been developed  over the
 past five years through a cooperative project between  the  IDEM and  USEPA.  Each  of Indiana's six
 ecoregions have been investigated to determine "least impacted"  conditions across current  ecoregion
 boundaries.  Recognizing the possibility that no areas in Indiana's landscape  have escaped dramatic alteration
 since the industrial revolution, efforts to characterize  the  best attainable conditions  for the biological

-------
 Biological Characterization
community  have evaluated fish community  structure and function.  The  IBI, as a multi-metric index, was
developed for warmwater stream  fish communities in Indiana and Illinois and uses characteristics  (termed
"metrics") of the community  to identify important aspects.

        Purpose and Scope

This report  presents the investigative design, methodology, and  results from the sampling of twelve locations
in the  Middle Fork Anderson  River. The criteria used for evaluating the  biological integrity of the streams
are based on interim criteria for the Interior River Lowland ecoregion, Ohio River subdivision. Criteria is
determined  from extensive sampling across the Interior  River Lowland (as defined by Omernik and Gallant
1988) which was used  to score the metric expectations for characteristics  requiring drainage area  calibration.
Stations were selected which corresponded  to previous investigations by the USEPA  and Indiana  Department
of Natural Resources (IDNR).  The following study objectives are considered:

        1) What is the current status of the aquatic communities  of the Middle Fork Anderson River
        watershed? The fish community was used as an environmental  indicator or direct measure
        of the aquatic health  of the system since the data analysis component  of the project was less
        intensive than using  the  macroinvertebrate  community.  Also, the fish community evaluates
        data over a longer time frame  due to life history aspects and ability of the fish communities
        to assimilate information  over larger spatial scales.

        2) Are the appropriate designated uses assigned to the Middle Fork Anderson River? The
        designated  use for the Middle Fork Anderson River is general use.  This suggests that the
        stream  should be capable of supporting a warmwater fish community.

        3) Does the species assemblage of the Middle Fork Anderson River possess any unique or rare
        species which are either receiving State or Federal protection? Species diversity was evaluated
        by a comparison of threatened  or endangered species listed under the protection of the
        Federal Endangered  Species Act and State of Indiana List of Endangered,  Threatened, and
        Special Concern taxa (Simon et al. 1992).

        Description of the Study Area

The Middle Fork Anderson River is a small watershed found in extreme southern Indiana.  The watershed
lies primarily in Perry  County and forms the border  between the Interior  River Lowland  and the Interior
Plateau ecoregions  as defined by Omernik and Gallant (1988). The  ecoregion consists of dissected glacial till
plain which  is covered by thick mantle loess, rolling narrow  ridgetops, and hilly to steep ridge and  valley
slopes.  The Interior River Lowland is an area  of varied  land use including forestry, diverse cropland
agriculture,  orchards, livestock and oil and gas  production. The Middle Fork drains 69,400acres and lies on
the edge of the  Crawford Upland  (USDA  1969).  Major land use in the drainage is woodland  (46%),
cropland (25%), and grassland (22.5%). Nearly 20% of the land is publicly owned  by the U.S. Forest Service
and managed  for public fishing (Hottell 1980).

The Middle Fork Anderson River is a tributary  of the Anderson River and has  a drainage area of 106 miles2
(Hoggatt 1975). The Middle Fork begins in Crawford County but is primarily contained  in Perry  County.
The study area for this investigation was concentrated between  Bristow and the confluence of the  Middle
Fork with the  Anderson River. The river flows southwest across northwestern  and  central Perry County.

-------
                                                                          Middle Fork Anderson River
The primary land use is agricultural and livestock production.

Stations selected for the current investigation correspond to historical collections made by the IDNR and
EPA during the last decade.  Twelve locations  (Fig. 1) were sampled  in the Middle Fork Anderson River (6
sites) and select tributaries  (Sulphur Fork (2);  Kraus Creek (2); and Theis Creek  (2)) to determine fish
community integrity.

From upstream to downstream, the stations include Perry County (station 94170): Middle Fork Anderson
River, at SR 145 bridge, Bristow, Clark Twp. T 4W R 3W  S 27; Perry County (station 94171): Middle Fork
Anderson River, 2 mi S Bristow, Anderson Twp. T 5S R 3W S 4; Perry County (station  94179): Middle Fork
Anderson River, 2.75 mi S  Bristow, Anderson Twp. T 5S R 3W S 9; Perry County (station 94174): Sulphur
Fork, at SR 145 bridge, 2.5 mi S Bristow, Anderson Twp. T 5S R 3W S 11; Perry County (station 94173):
Sulphur Fork, 1.5 mi W SR 145 off dirt road, 3 mi SE Bristow, Anderson  Twp. T 5S R 3W S 12; Perry
County (station 94175): Kraus Creek, at SR  145 bridge, 9 mi N Cannelton, Anderson Twp. T 5S R 3W S 26;
Perry County (station 94176): Kraus Creek, at  CR  82 bridge, 6.75 mi  S Bristow, Anderson Twp. T 5S R 3W
R 33; Perry County (station 94177): Middle Fork Anderson River, 6.75 mi N Cannelton,  Anderson Twp. T
6S R 3W S 5; Perry County (station 94178): Middle Fork Anderson River, Lincoln's Ford, 6 mi S Bristow,
Anderson Twp. T 5S R 3W S 28; Perry County (station 94172) Middle Fork Anderson River, at CR  116
bridge, 4.125 mi S Bristow, Anderson Twp. T 5S R 3W S 15; Perry County (station  94180): Theis Creek, off
CR 116 at bridge 26,4.5 mi S Bristow, Anderson Twp. T 5S R 3W S  22; and Perry County (station 94181):
Theis Creek,  at SR 145 bridge, 4.5 mi S Bristow, Anderson Twp. T 5S R 3W S 23.

Methods

        Survey design

Sample  locations were selected based on historical'collection events in the Middle Fork  Anderson  River.
Shelby Gerking was the first to collect from  a single location in the Middle Fork during his investigation of
Indiana fish distribution during the mid  1940's (Gerking  1945).  Stream  surveys were collected by Indiana
Department of Natural Resources personnel during the early  1970's(Keller 1971), early  1980's(Hottell
1980), and late 1980's(Stefanavage 1989) and early 1990's(T. Simon, unpublished data).  Stefanavage has
conducted  additional surveys of the impoundments  during  the last five years (T. Stefanavage,  personal
communication).

         Sample collection

Qualitative Habitat Evaluation Index (QHEI): Habitat evaluation  was completed in conjunction with the fish
survey. The  QHEI, developed by Ohio  EPA (1987), provides numerical assignments for six criteria to assess
riffle and pool  quality. Scoring incorporates substrate quality, instream cover, channel morphology, riparian
zone and bank erosion, and pool and riffle quality based on drainage  area.  The QHEI also has a component
which rates stream gradient since many features  of the habitat are determined  by critical flow velocities.
This metric was eliminated  from our evaluation since the gradient throughout this watershed  was low to
moderate and typical of the Interior River Lowland. Since results were only being compared  within a narrow
range this did not matter for our analysis.  Based on information derived from the Ohio EPA, a QHEI  score
of 75 is considered exceptional and meeting Clean Water Act goals.

Fish Community Structure and Function: Standard  operating procedures of the EPA, Region  5, for conducting

-------
 COUTH. MIIE
 n s rir,f.'.sutt>utK
 CIVIL [OWIISWP llll£
 SEniOi'l HUE
 SECTtlTN NUMBER
 PW0 FIOAD
 GRAVE! flOAD
 Dili I BOAQ
 FARM HOAR
 WIEBSI/HtHIRHW/iV
 BRIDGE
 DUllOIIIG
 SCI 1001
 ciiuncii
 CEME1ERV
 PEHENWIAL STREAM
 IHlLnMllTEH! SIHtAM
 WATEIiSIIEDBOUHDAitV
 linAIHAriE AI1EA CON fROLLED
 BV smucTimE
DRAIHAGE AREA COMIflOLLEB
BY EXISTING STRUCTURE
DRAINAGE AR6A ACREAGE

EXISTING STRUCTURE

Fl OODWATER RETARDING STRUCTURE

MUlTIPLF PURPOSE STRUG HIRE

ftEACII

SIRUCTlIRt NUMBER
IFTTFHS INDICATE PURPOSE
 UPWATER SUPPLY
   HECHEATION
CHANNEL WORK (COMPt El ED)
 Cl EARING, SHAGGING A SlIltAI REMOVAL
 CHAUNEL EXCAVATION
CHANNEL WORK (PLANNED)
 DEBHIS & SflOM REMOVAI
 CflAHMEl  EXCAVATION
 CLEARING, SNAGGING 4 SHOAL REMOVAL
               Figure 1.  Project Map showing the location of sampling locations of the Middle Fork Anderson River.
                                                             MIDDLl  FORK  OF ANDliSOlM RIVER  WATERSHED
                                                                                    CRAWFORD AND PERRY COUNTIES
                                                                                                 IMDIAWA

-------
Biological Characterization
Fig. 1.  Project Map showing the location of sampling locations on the Middle Fork Anderson  River

-------
                                                                             Middle Fork Anderson River
Rapid  Assessment of Ambient Water Quality using Fish (EPA  1988) were used.  The methods are based on
those described  for fish by Ohio  EPA (1987), Karr (1981), and  Karr et al. (1986) for evaluating midwestern
warmwater streams.   Electrofishing collection gear was employed to collect a "representative" collection of
species occurring within a stream reach.  Leopold et al. (1964) in evaluating various sized streams in North
America  found that major  habitat cycles (i.e. riffle, run, pool) were repeated every 15 stream widths.  Sample
distances were based  on 15 times the stream width with a  minimum sample distance of 50 m.  Since all
stream segments were wadable, a long-line electrofishing unit comprised  of a T&J pulsed D-C unit capable
of 1750 watt  output was fished in an upstream  direction, output was 300 volts and approximately  6-8 amps.
Collected specimens were placed in a live well, identified  to  species, measured for bulk  weight and length
ranges for each  species, and released.

Stream community health was evaluated using the Index of Biotic Integrity (Karr  et al.  1986).  The IBI relies
on multiple attributes based on community concepts, to evaluate complex systems. The IBI incorporates
professional judgement  in a systematic  and sound manner, but sets quantitative criteria  that enables
determination of biological  integrity based on species richness and composition, trophic  composition,
reproductive  guilds, and fish abundance and  individual condition.  The IBI  metrics are a modification of
Karr's original (Table 1) to better evaluate important community  attributes of Interior River Lowland
streams.   The Indiana version of the  IBI  for the Interior River Lowland retains the same precepts and
evaluation ability as  Karr's  original. Metrics reflect insights  from several perspectives and cumulatively are
responsive to changes of relatively small magnitude, as  well as broad ranges of environmental degradation.
Since metrics respond differentially to various  perturbations  (e.g.,siltation versus  toxic chemicals), as well as
to various levels within the  ranges of integrity, conditions can be determined with considerable  accuracy.  The
interpretation of IBI  numerical scoring is incorporated  into six narrative categories (Table  2).

The IBI  requires calibration in order to provide accurate  depictions of reference condition within a region
(Hughes  1995). Reference  conditions were defined from areas  of least impact from anthropogenic activities.
Although reference sites may not exist in Indiana, subtle aspects of community structure and function are
exhibited in streams with minimal impact. By evaluating  aspects of the community function the structural
attributes of the community emerges from a large  database.  Several of the metrics are  drainage  size
dependent and  require  the  selection of numerical expectations in order to score metrics.  The EPA  in
cooperation  with IDEM are providing  reference  conditions for  each of the recognized regions  in Indiana
(Simon  1991; Simon  1992;  Simon 1994).  Sampling of the  Interior River Lowland ecoregion was completed
during 1994. Interim criteria are presented  in Table 2 for the Ohio River subdivision.  This  area  would
encompass the  Middle Fork Anderson  River.  Interim  criteria are presented for small headwater  streams less
(< 20 mi2) and for wadable streams  (> 20-1000mi2).  For this analysis results for the maximum species
richness lines are presented for  stations between 10-20 mi2 and for streams between  80-100 mi2.  The
reference database contained stream segments which generally  had  an intact riparian corridor; was free from
toxic pollutant influence and major point source dischargers; lacked obvious diffuse non-point source impacts,
 such as  agriculture and silviculture; and generally had consistent habitat cycles representative  of the region.

 Metrics  used for the current study are modifications of the original IBI  (Karr  1981) and more closely reflect
 accepted changes designed by Ohio Environmental  Protection Agency (1987) and Simon (1991) for the
 Central  Corn Belt Plain.

-------
Biological Characterization
Table 1.  Interim  Scoring Criteria for 12 IBI metrics for low to moderate  gradient stream systems within the
         Interior River Lowland for the Ohio River subsection.
Metric
Category
Metric
     Scoring Criteria by Drainage  Area
< 20 mi2               > 20-100 mi2
Species
Composition
Total number of species         6-12

Number of darter/sculpin/
madtom species                 1-2
Number of darter species

% Headwater species           13-26%
Number of sunfish species

Number of minnow species      3-6
                       7-16
                                                                      1-3
                                                                     2-3


Trophic
Composition


Fish
Condition


Number of sucker species
Number of sensitive species 2-4
% Tolerant species 25-50%
% Omnivores 18-33%
% Insectivores 25-50%
% Pioneer Species 22-48%
% Carnivores
Catch per unit effort 125-275
% Simple lithophils 16-34%
% DELT anomalies 0.1-1.3%
1-3
3-5
25-50%
18-33%
25-50%
2-5%
150-300
16-34%
0.1-1.3%
' Special scoring procedures required when less than 25 (headwater)  or 50 individuals (wadeable) are
collected.

-------
                                                                             Middle Fork Anderson River
Table 2. Biotic Integrity classes used  in assessing fish communities along with general descriptions  of their
         attributes (Karr et al. 1986).
Total IBI
Score
Integrity
Class
Attributes
58-60
48-52
40-44
28-34
 12-22
Excellent        Comparable to the best situation without human disturbance; all regionally
                expected species for the habitat  and stream size, including the most
                intolerant forms are present with a full array of age (size) classes; balanced
                trophic  structure.

Good           Species  richness somewhat below expectation, especially due to the loss of
                the most intolerant forms; some species are present with less than optimal
                abundance  or size distributions;  trophic structure shows some signs of
                stress.

Fair            Signs of additional deterioration  include loss of intolerant forms, fewer
                species, highly skewed trophic structure (e.g. increasing frequency of
                omnivores  and other  tolerant species); older age classes of top predators
                may be  rare.

Poor            Dominated  by omnivores, tolerant forms, and habitat generalists; few top
                carnivores; growth rates and condition factors commonly depressed;  hybrids
                and diseased fish often present.

Very Poor      Few fish present, mostly introduced  or  tolerant  forms; hybrids common;
                disease, parasites,  fin damage, and other anomalies regular.

No fish         Repeated  sampling finds no fish.
Results
         Biological Integrity
 The general  ecological health of the Middle Fork Anderson River is in the "Good" narrative category.
 Biological integrity improves downstream  with a low rating of "Fair-Poor" in  Bristow (station  94170; IBI  =
 36) and an "Excellent" rating at the lowermost station upstream  of the mouth of the Anderson River (station
 94177; IBI = 57).  Middle reaches of the  study zone typically scored in the "Fair-Good" category.  Declines
 in biological integrity in the upper portions of the Middle Fork may be attributed  to past channelization
 events upstream  of Bristow and the movement  of suspended  sediment and bedload  through the system.
 Evidence of this effect is the low proportion of simple lithophilic (broadcast  and egg-burying  species
 requiring rock substrates)  species from Bristow to the County Road 116 bridge (station 94179).  The lack of
 substrate  heterogeneity also reduced habitat for sensitive species.  Removal of shoals and riffle habitats also
 reduced  the number  of darter  species at Bristow.

-------
 Biological Characterization
  A.  Middle  Fork  Anderson  River
   Trends  in  Biological  Integrity
      B.  Tributaries  Middle  Fork  Anderson
       River—Trends  in  Biological   Integrity
cO r
50 t
 ~0
                                             IIUI
1970

198O

1989

^992

1994
           23      5O      75

             DRAINAGE APEA  (SO.
                                   100
                                          125
                                                          CD
GO
<
4O'
20
20
1O
0
19

. fc- • . _. 	
£5 = = - \ 	 y^
S. »fc^»— ^^^^^^




iM
71 198O 1989 1992 1994
YEAR
     SUtfxr For
     Dirt Road
••••• SulpKr Rx
     SR 145
     Thele  Cree
     CO 1  16
     Kraua Oet
     SH 145
     KrauB Cre«
     CR 82
 Three  tributaries of the Middle Fork Anderson River had biological integrity scores typically in the "Fair"
 rating  category. Biological integrity  improved downstream for Sulphur Fork and Theis Creek  and did not
 vary appreciably for Kraus Creek.  Each tributary segment had two sampling points.  Both Kraus and Theis
 Creek  lacked species  typical of headwater stream segments, while the upstream  site on Theis Creek and both
 stations on Kraus Creek had high incidence of pioneer species.  Pioneer  species increase in areas where
 water  quantity  is limiting and are the first species to  recolonize an area because of the tolerant nature of the
 species and ability to  endure harsh environments. The simple lithophilic species metric  also reflected
 lowered expectations  probably as a result of the  lack of flow during periods of the year.

 Habitat ratings based on the QHEI  indicate a reduction of available habitat in the  stream segment between
 Bristow (station 94170; QHEI = 60.3) and the middle zone (station  94172; QHEI = 40.6). Station 94172
 also had the lowest habitat quality observed in the Middle Fork (Table 3).  The lower three stations all met
 Ohio EPA habitat criteria with scores above 75 (94179 = 75.4,94178 = 78.3,and 94177  = 82).  Lowered
 scores in the upper three stations were  a result of substrate quality, lack of riffles, and the evidence of bank
 erosion. Habitat quality declined downstream in Sulphur Fork, remained  consistent in Theis  Creek, and
 improved marginally  in Kraus Creek. Lower Sulphur Fork (station 94-173, QHEI = 32.3) and both sites on
 Kraus  Creek reflected a stream channel recovering from a previous channelization  events.  The detrimental
 activities associated with clearing, snagging, and  shoal removal prevent biological communities from
 inhabiting disturbed segments.  Stream  segments affected by high to moderate  influence  from channel
 modification include  the Middle Fork Anderson  River (stations  94172,94171,94170); Sulphur Fork (station
 94173); and Kraus  Creek (stations  94176 and 94175).

 Evaluation of trends  in the Middle Fork Anderson River from 1971 to 1994 show that biological integrity has
 improved with time and longitudinal distance downstream  (Fig. 2A).  Improvement in biological integrity has
 been observed in each year monitoring  has been conducted.  The largest improvements  in biological integrity
 has been observed  in the lowermost  Middle Fork Anderson River site (station  94177.increase of 20 IBI
 points between  1970  and  1994). IBI  ratings for the station  has shown an  improvement from "Poor-Fair"
 during 1970 to a rating of "Excellent" in 1994. This station had  the best habitat  of all Middle Fork Anderson
 River  locations.
                                                    8

-------
Table 3. Matrix of QHEI scores and corresponding natural and modified channel habitat attributes
from the Middle Fork Anderson River watershed during 1994.





Site
Number QHEI
Natural Channel Attributes

ll ! • i I
jiilfjjjijl
J^ 2 £ £•- = z a" °
j^^iillliiii
Modified Channel Attribute
High Influence Moderate Influence
F I I
Je 4"3§>^7l5«^.|ll§|'
-------
Biological Characterization
Biological integrity of the tributaries of the Middle Fork have been  relatively stable between  1971-1994, with
the exception of the downstream Sulphur Fork location (94173; Fig. 2B).  The upstream  Sulphur Fork
location between 1980 and 1989 declined substantially (ca. 10 IBI points) in biological integrity. Only
Sulphur Fork at the SR  145 bridge (station 94174) has shown any improvement in biological integrity (period
between  1992-1994). Since no major point source dischargers occur in the watershed, differences in biological
integrity of the Middle Fork can be attributed to changes in landuse and channelization.

The number of species collected between Hottell's investigation during the 1980'sand 1994 show a reduction
in diversity. The reduction in diversity can only be attributed to largescale landuse disturbance in the
watershed. The construction of impoundments reduce migration and recolonization potential of upstream
tributaries during extremes in flow conditions.  These barriers  reduce any fish species from occupying
tributaries during drought conditions, likewise the degradation  of habitat as a result of channel excavation,
shoal  removal,  and clearing and snagging activities only accentuates  problems during high flow events and
result in  flooding.  The importance of riparian  wetlands  for the decrease in nutrient transport,  decrease of
erosion, importance to certain  sensitive fish species, and the stabilization of banks during high  flow events
cannot be overstated.  Best Management  Practices must include the protection  of these vital riparian  wetland
areas  for the continued existence of some endangered species including aquatic and terrestrial  communities.
        Designated  Uses

The Middle Fork  Anderson River is a general use stream  in the state of Indiana.  Based on the Index of
Biotic Integrity results the Middle Fork is typical of streams classified as a general use stream.   Since the
lower portions of  the Middle Fork Anderson  River reflect near exceptional attributes  of the Interior River
Lowland, a use attainability analysis should be conducted to determine  if the reach should receive additional
protection  from encroaching land management practices.  Channelization of tributary  segments and the
Middle  Fork proper will reduce available habitats,  decrease riparian corridor and instream  habitat for aquatic
and terrestrial organisms, and cause the reduction  and  loss of sensitive species.
References

Hoggatt, R.E. 1975. Drainage Areas of Indiana Streams. U.S. Geological Survey, Indianapolis, Indiana.

Hottell, H.E.  1980. Middle  Fork Anderson  River, Perry and Crawford Counties Stream  Survey report.
Indiana Department  of Natural  Resources,  Indianapolis, Indiana. Unpublished report 27 pp.

Hughes, R.M. 1995. Determination of reference conditions, in W.S. Davis and T.P. Simon. Biological Criteria
and  Assessment: Tools for  Water Resource Decision Making.  Lewis Publishers, Ann Arbor, Michigan.

Karr, J.R.  1981. Assessment of  biotic integrity using fish communities. Fisheries 6:21-27.

Karr, J.R..K.D. Fausch, P.L. Angermeier, P.R. Yant, and  I.J. Schlosser. 1986. Assessing biological integrity
in running  waters: a method and its rationale.  Illinois Natural History Survey, Special Publication 5. 28 pp.

Karr, J.R. and D.R. Dudley. 1981. Ecological perspective on water quality goals. Environmental  Management
5: 55-68.
                                                   10

-------
                                                                          Middle Fork Anderson River
Keller, C.R. 1971. Middle Fork Anderson River, Stream  Survey Report, Perry and Crawford Counties.
Indiana  Department of Natural Resources, Indianapolis,  Indiana.  Unpublished report.

Leopold, L.B..M.G. Woolman, and J.P. Miller. 1964. Fluvial processes in Geomorphology.  W.H. Freeman,
San Francisco, California.

Ohio Environmental Protection Agency. 1987. Biological criteria for the protection of aquatic life: Volume II.
Users manual  for biological field assessment  of Ohio  surface waters.  Ohio Environmental Protection Agency,
Columbus.

Omemik, J.M. and A.L. Gallant. 1988. Ecoregions of the upper Midwest States. U.S. Environmental
Protection Agency, Environmental  Research  Laboratory, Corvallis, Oregon. EPA 600/3-88/037.

Simon, T.P. 1991. Development of Index of Biotic Integrity expectations for the ecoregions  of Indiana.
I. Central Corn Belt Plain. U.S. Environmental  Protection Agency, Region 5, Chicago, Illinois. EPA 905/9-
91/025.

Simon, T.P. 1992. Biological criteria development for  large rivers  with an emphasis on an assessment of the
White River drainage, Indiana. U.S. Environmental Protection Agency, Region 5, Chicago,  Illinois. EPA
905/R-92/006.

Simon, T.P. 1994 Development of Index of Biotic Integrity  expectations for the ecoregions of Indiana.
II. Huron-Erie Lake Plain. U.S. Environmental  Protection Agency, Region 5, Chicago, Illinois. EPA 905/R-
92/007.

Simon, T.P..J.O. Whitaker, Jr, J.S. Castrale,  and  S.A. Minton. 1992. Checklist of the vertebrates  of Indiana.
Proceedings of the Indiana Academy of Science 101:95-126.

Stefanavage, T.C. 1993. Fisheries Survey of the  Anderson River watershed, 1989 fish management report.
Indiana  Department of Natural Resources, Division of Fish and Wildlife, Indianapolis, Indiana. Unpublished
report 58 pp.

U.S. Department  of Agriculture. 1969. Soil Survey, Perry County, Indiana. Soil Conservation and Forest
Service. 70 pp.

U.S. Department  of Agriculture, Soil Conservation Service.  1980. Environmental  Impact Assessment- Middle
Fork Anderson River. U.S. SCS, Indiana.
                                                  11

-------
Biological Characterization
Appendix I. Metric scoring for individual stations sampled within the Middle Fork Anderson River.
Site 94170
Perry Co: Middle Fork Anderson River, at SR 145 bridge, Bristow, Clark Twp. T 4S R 3W S 27
Drainage area: 39.8 mi2
Metric
Category
Metric
                                    Scoring Criteria
Actual
IBI Score
Species
Composition
Trophic
Composition
Fish
Condition
Total number of species

Number of darter/sculpin/
madtom species
Number of darter species

% Headwater species
Number of sunfish species

Number of minnow species
Number of sucker species

Number of sensitive species

% Tolerant species


% Omnivores

% Insectivores

% Pioneer Species
% Carnivores


Catch per unit effort

% Simple lithophils

% DELT anomalies
2

1

10.3%


24%

51.7%


20.7%


29

6.8%

0

Total  IBI Score
3

1

5


3

5
1

1

5
                                                                     36
                                                 12

-------
                                                                        Middle Fork Anderson River
Site 94171
Perry Co: Middle Fork Anderson  River, 2 mi S Bristow, Anderson Twp. T 5S R 3W S 4
Drainage area: 43.8mi2
Metric
Category
Metric
                                    Scoring Criteria
Actual
IBI Score
Species
Composition
Fish
Condition
Total number of species

Number of darter/sculpin/
madtom species
Number of darter  species

% Headwater species
Number of sunfish species

Number of minnow species
19



Trophic
Composition

Number of sucker species
Number of sensitive species
% Tolerant species

% Omnivores
% Insectivores
2
1
1.2%

0
85.7%
3
1
5

5
. 5
                % Pioneer Species
                % Carnivores
Catch per unit effort

 % Simple lithophils

 % DELT anomalies
14.3%                 5


84                     1

16.7%                 3

0                      5

Total IBI Score         46
                                                 13

-------
Biological Characterization
Site 94172
Perry Co: Middle Fork Anderson River, 2.75 mi S Bristow, Anderson Twp. T 5S R 3W S 9
Drainage area: 44.8 mi2
Metric
Category
Metric
                                    Scoring Criteria
Actual
IBI Score
Species
Composition
Fish
Condition
Total  number of species

Number of darter/sculpin/
madtom species
Number of darter species

% Headwater species
Number of sunfish species

Number of minnow species
 13



Trophic
Composition

Number of sucker species
Number of sensitive species
% Tolerant species
% Omnivores
% Insectivores
0
2
1.9%
1.9%
91.4%
1
1
5
5
5
                % Pioneer  Species
                % Carnivores
Catch per unit effort

% Simple lithophils

% DELT anomalies
6.7%                   5


105                    1

0%                    1

0                      5

Total IBI Score         40
                                                14

-------
                                                                        Middle Fork Anderson River
Site 94173
Perry Co: Sulphur Fork, at SR  145 bridge, 2.5 mi S Bristow, Anderson Twp. T 5S R 3W S 11
Drainage area: 27.1 mi2
Metric
Category
Metric
                                    Scoring Criteria
Actual
IBI Score
Species
Composition
Total number of species

Number of darter/sculpin/
madtom species
Number of darter species

% Headwater species
Number of sunfish species

Number of minnow species
18



Trophic
Composition

Number of sucker species
Number of sensitive species
% Tolerant species
% Omnivores
% Insectivores
1
2
18.8%
14.6%
79.7%
3
1
5
5
5
 Fish
 Condition
                %  Pioneer Species
                %  Carnivores
 Catch per unit effort

 % Simple lithophils

 % DELT anomalies
3.1%                  3


192                   3

1.6%                  1

0                     5

Total IBI Score         44
                                                  15

-------
Biological Characterization
Site 94174
Perry Co: Sulphur Fork, 1.5 mi W SR 145 off dirt road, 3 mi SE Bristow, Anderson Twp. T 5S R 3W S 12
Drainage area: 23.2mi2
Metric
Category
Metric
                                    Scoring Criteria
Actual
IBI Score
Species
Composition
Fish
Condition
Total number of species

Number of darter/sculpin/
madtom species
Number of darter species

% Headwater species
Number of sunfish species

Number of minnow species
 16



Trophic
Composition

Number of sucker species
Number of sensitive species
% Tolerant species
% Omnivores
% Insectivores
1
4
42.3%
41.8%
57.2%
3
3
3
1
5
               % Pioneer Species
               % Carnivores
Catch per unit effort

% Simple lithophils

% DELT anomalies
1.0%


194

1.5%

0

Total IBI Score
                                                                    38
                                                16

-------
                                                                       Middle Fork Anderson River
Site 94175
Perry Co: Kraus Creek, at SR 145 bridge, 9 mi N Cannelton, Anderson Twp. T 5S R 3W S 26
Drainage area: 6.2 mi2
Metric
Category
Metric
                                   Scoring Criteria
Actual
IBI Score
Species
Composition
Total number of species         13

Number of darter/sculpin/
madtom species                1
Number of darter species

% Headwater species           0.3%
Number of sunfish species

Number of minnow species      4
Number of sucker species
Number of sensitive species 2

Trophic
Composition


Fish
Condition


% Tolerant species
% Omnivores
% Insectivores
% Pioneer Species
% Carnivores
Catch per unit effort
% Simple lithophils
% DELT anomalies
25.3%
21.5%
59.2%
43.6%
316
0.1%
0
Total IBI Score
3
3
3
5
3
5
1
5
40
                                                 17

-------
Biological Characterization
Site 94176
Perry Co: Kraus
Drainage area: 8
Metric
Category
Species
Composition





Trophic
Composition


Fish
Condition



Creek, at CR 82 bridge, 6.75 mi
.2 mi2
Metric
Total number of species
Number of darter/ sculpin/
madtom species
Number of darter species
% Headwater species
Number of sunfish species
Number of minnow species
Number of sucker species
Number of sensitive species
% Tolerant species
% Omnivores
% Insectivores
% Pioneer Species
% Carnivores

Catch per unit effort
% Simple lithophils
% DELT anomalies


S Bristow, Anderson Twp. T 5S R 3W S 33
Scorine Criteria
Actual
13
2
0
4
2
34.8%
30.4%
69.5%
36.4%

187
0.5%
0
Total IBI Score
IBI Score
5
3
1
3
3
3
3
5
3

3
1
5
38
                                               18

-------
                                                                         Middle Fork Anderson River
Site 94177
Perry Co: Middle Fork Anderson River, 6.75 mi N Cannelton, Anderson Twp. T 5S R 3W S 5
Drainage area: 105 mi2
Metric
Category
Metric
                                    Scoring Criteria
Actual
IBI Score
Species
Composition
Fish
Condition
Total number of species

Number of darter/sculpin/
madtom species
Number of darter species

% Headwater species
Number of sunfish species

Number of minnow species
23



Trophic
Composition

Number of sucker species
Number of sensitive species
% Tolerant species

% Omnivores
% Insectivores
3
5
18.8%

16.0%
81.1%
3
3
5

5
5
                % Pioneer Species
                % Carnivores
Catch per unit effort

 % Simple lithophils

 % DELT anomalies
2.6%                  3


313                    5

57.5%                 5

0                      5

Total IBI Score         57
                                                 19

-------
Biological Characterization
Site 94178



Perry Co: Middle Fork Anderson River, at Lincoln's Ford, 6 mi S Bristow, Anderson Twp. T 5S R 3W S 28
Drainage area: 92.5 mi2
Metric
Category
Species
Composition





Trophic
Composition


Fish
Condition


Metric
Total number of species
Number of darter/ sculpin/
madtom species
Number of darter species
% Headwater species
Number of sunfish species
Number of minnow species
Number of sucker species
Number of sensitive species
% Tolerant species
% Omnivores
% Insectivores
% Pioneer Species
% Carnivores

Catch per unit effort
% Simple lithophils
% DELT anomalies
Scoring Criteria
Actual
22
2
3
3
4
23.5%
22.3%
75.9%
0.4%

651
42.9%
0
Total IBI Score

IBI Score
5
3
3
3
3
5
3
5
1

5
5
5
46
                                                20

-------
                                                                        Middle Fork Anderson River
Site 94179
Perry Co: Middle Fork Anderson  River, at CR 116 bridge, 4.125 mi S Bristow, Anderson Twp. T 5S R 3W
S IS; Drainage area: 79.6 mi2
Metric
Category
Metric
                                    Scoring Criteria
Actual
IBI Score
Species
Composition
Total number of species

Number of darter/sculpin/
madtom species
Number of darter species

% Headwater species
Number of sunfish species

Number of minnow species
16

Number of sucker species
2
Number of sensitive species 3

Trophic
Composition


Fish
Condition


% Tolerant species
% Omnivores
% Insectivores
% Pioneer Species
% Carnivores

Catch per unit effort
% Simple lithophils
% DELT anomalies
20.1%
15.9%
58.9%
25.2%

214
1.9%
0
Total IBI Score
3
3
5
5
5
5

3
1
5
46
                                                21

-------
 Biological Characterization
 Site 94180
 Perry Co: Theis Creek, off CR 116 at bridge 26,4.5 mi S Bristow, Anderson Twp. T 5S R 3W S 22
 Drainage area:  9.5 mi2
Metric
Category
 Metric
                                     Scoring Criteria
Actual
IBI Score
Species
Composition    Total number of species

                Number of darter/sculpin/
                madtom species
                Number of darter species

                % Headwater species
                Number of sunfish species

                Number of minnow species
                Number of sucker species

                Number of sensitive species

                % Tolerant species

Trophic
Composition    % Omnivores

                % Insectivores

                % Pioneer  Species
                % Carnivores
Fish
Condition
Catch per unit effort

% Simple lithophils

% DELT anomalies
                               12
3
12.0%
10.2%
89.2%
13.9%
166
4.8%
0
Total IBI Score
3
5
5
5
5
3
1
5
43
                                                22

-------
                                                                        Middle Fork Anderson River
Site 94181
Perry Co: Theis Creek, at SR  145 bridge, 4.5 mi S Bristow, Anderson Twp. T 5S R 3W S 23
Drainage area: 6.5 mi2
Metric
Category
Species
Composition
Metric
Total number of species
Scoring Criteria
Actual
18

IBI Score
5
               Number of darter/sculpin/
               madtom species
               Number of darter species

               % Headwater species
               Number of sunfish species

               Number of minnow species
               Number of sucker species
0.5%
Number of sensitive species 4

Trophic
Composition


Fish
Condition


% Tolerant species
% Omnivores
% Insectivores
% Pioneer Species
% Carnivores
Catch per unit effort
% Simple lithophils
% DELT anomalies
31.7%
30.8%
46.9%
59.6%
441
6.0%
0
Total IBI Score
3
3
3
3
1
5
1
5
36
                                                 23

-------
Biological Characterization
Site 1 (Keller 1971)
Perry Co: Middle Fork Anderson River, 6.75 mi n Cannelton, Anderson Twp. T 5S R 3W S 5
Drainage area:  105 mi2
Metric
Category
Metric
                                    Scoring Criteria
Actual
IBI Score
Species
Composition
Fish
Condition
Total  number of species

Number of darter/sculpin/
madtom species
Number of darter species

% Headwater species
Number of sunfish species

Number of minnow species
 16



Trophic
Composition

Number of sucker species
Number of sensitive species
% Tolerant species
% Omnivores
% Insectivores
1
3
34.0%
30.4
59.0%
3
3
3
3
5
                % Pioneer Species
                % Carnivores
Catch per unit effort

% Simple lithophils

% DELT anomalies
 1.7%                  1


1118                   5

4.5%                   1

0                      5

Total IBI Score         38
                                                24

-------
                                                                         Middle Fork Anderson River
Site 1 (Hottell  1980)
Perry Co: Middle Fork Anderson River, 6.75 mi n Cannelton, Anderson Twp. T 5S R 3W S 5
Drainage area: 105 mi2
Metric
Category
Metric
                                    Scoring Criteria
Actual
IBI Score
Species
Composition
Fish
Condition
Total number of species

Number of darter/sculpin/
madtom species
Number of darter species

% Headwater species
Number of sunfish species

Number of minnow species
22



Trophic
Composition

Number of sucker species
Number of sensitive species
% Tolerant species

% Omnivores
% Insectivores
4
5
34.2%

15.4%
66.5%
5
3
3

5
5
                % Pioneer Species
                % Carnivores
Catch per unit effort

% Simple lithophils

% DELT anomalies
 7.5%                  5


 663                   5

25.8                   3

0                      5

Total IBI Score         48
                                                 25

-------
Biological Characterization
Site 1 (Stefanavage  1989)
Perry Co: Middle Fork Anderson River, 6.75 mi n Cannelton, Anderson Twp. T 5S R 3W S 5
Drainage area:  105 mi2
Metric
Category
Metric
                                    Scoring Criteria
Actual
IBI Score
Species
Composition
Fish
Condition
Total number of species

Number of darter/sculpin/
madtom species
Number of darter species

% Headwater species
Number of sunfish species

Number of minnow species
 18



Trophic
Composition

Number of sucker species
Number of sensitive species
% Tolerant species
% Omnivores
% Insectivores
5
3
25.556
19.3%
71.2%
5
4
3
3
5
                % Pioneer Species
                % Carnivores
Catch per unit effort

% Simple lithophils

% DELT  anomalies
 8.5%


 212

48.6

0

Total IBI Score
3

5

5

48
                                                26

-------
                                                                        Middle Fork Anderson River
Site 2 (Keller 1971)
Perry Co: Kraus Creek, at CR 82 bridge, 6.75 mi S Bristow, Anderson Twp. T 5S R 3W S 33
Drainage area: 8.2 mi2
Metric
Category
Metric
                                    Scoring Criteria
Actual
IBI Score
Species
Composition
Total number of species         15

Number of darter/sculpin/
madtom species                2
Number of darter species

% Headwater species           0.3%
Number of sunfish species

Number of minnow species      3
Number of sucker species
Number of sensitive species 2

Trophic
Composition


Fish
Condition


% Tolerant species
% Omnivores
% Insectivores
% Pioneer Species
% Carnivores

Catch per unit effort
% Simple lithophils
% DELT anomalies
53.9%
48.9%
33.5%
20.0%

779
1.2%
0
Total IBI Score
3
5
1
3
5

5
1
5
40
                                                27

-------
Biological Characterization
Site 3 (Keller 1971)
Perry Co: Theis Creek, off CR 116 at bridge 26, 4.5 mi S Bristow, Anderson Twp. T 5S R 3W S 22
Drainage area: 9.5 mi2
Metric
Category
Metric
                                    Scoring Criteria
Actual
IBI Score
Species
Composition
Total  number of species

Number of darter/sculpin/
madtom species
Number of darter species

% Headwater species
Number of sunfish species

Number of minnow species
Number of sucker species
21
Number of sensitive species 2

Trophic
Composition


Fish
Condition


% Tolerant species
% Omnivores
% Insectivores
% Pioneer Species
% Carnivores

Catch per unit effort
% Simple lithophils
% DELT anomalies
15.6%
11.9%
48.1%
46.6%

320
1.9%
0
Total IBI Score
3
5
5
3
3

5
1
5
42
                                                28

-------
                                                                       Middle Fork Anderson River
Site 3 (Hottell  1980)
Perry Co: Theis Creek, off CR 116 at bridge 26,4.5 mi S Bristow, Anderson  Twp. T 5S R 3W S 22
Drainage area: 9.5 mi2
Metric
Category
Metric
                                   Scoring Criteria
Actual
IBI Score
Species
Composition
Total number of species         20

Number of darter/sculpin/
madtotn species                3
Number of darter species

% Headwater species           0.7%
Number of sunfish species

Number of minnow species      3
Number of sucker species
Number of sensitive species 2

Trophic
Composition


Fish
Condition


% Tolerant species
% Omnivores
% Insectivores
% Pioneer Species
% Carnivores

Catch per unit effort
% Simple lithophils
% DELT anomalies
22.0%
20.4%
77.8%
17.6%

437
4.1%
0
Total IBI Score
3
5
3
5
5

5
1
5
46
                                                29

-------
Biological Characterization
Site MNON (Stefanavage 1989)
Perry Co: Middle Fork Anderson River, at Lincoln's Ford, 6 mi S Bristow, Anderson Twp. T 5S R 3W S 28
Drainage area: 92.5 mi2
Metric
Category
Metric
                                    Scoring Criteria
Actual
IBI Score
Species
Composition
Fish
Condition
Total  number of species

Number of darter/sculpin/
madtom species
Number of darter species

% Headwater species
Number of sunfish species

Number of minnow species
16



Trophic
Composition

Number of sucker species
Number of sensitive species
% Tolerant species
% Omnivores
% Insectivores
3
4
7.2%
50.8%
79.7%
3
3
5
5
5
                % Pioneer Species
                % Carnivores
Catch per unit effort

% Simple lithophils

% DELT  anomalies
10.1%                  5


69                     1

8.7%                   1

0                      5

Total IBI Score         42
                                                 30

-------
                                                                        Middle Fork Anderson River
Site 5 (Keller 1971)
Perry Co: Middle Fork Anderson River, Anderson Twp. T 5S R 3W S 15
Drainage area: 44.8 mi2
Metric
Category
Metric
                                    Scoring Criteria
Actual
IBI Score
Species
Composition
Fish
Condition
Total number of species

Number of darter/sculpin/
madtom species
Number of darter  species

% Headwater species
Number of sunfish species

Number of minnow species
14



Trophic
Composition

Number of sucker species
Number of sensitive species
% Tolerant species
% Omnivores
% Insectivores
1
2
57.3%
52.4
35.4%
3
1
1
1
3
                % Pioneer Species
                % Carnivores
Catch per unit effort

% Simple lithophils

% DELT anomalies
 7.3%


82

6.1%

0

Total IBI Score
1

1

5
                                                                    30
                                                 31

-------
Biological Characterization
Site 5 (Hottell 1980)
Perry Co: Middle Fork Anderson  River, Anderson Twp. T 5S R 3W S 15
Drainage area: 44.8 mi2
Metric
Category
Metric
                                     Scoring Criteria
 Actual
IBI Score
Species
Composition
Fish
Condition
Total number of species

Number of darter/sculpin/
madtom species
Number of darter species

% Headwater species
Number of sunfish species

Number of minnow species
 19



Trophic
Composition

Number of sucker species
Number of sensitive species
% Tolerant species
% Omnivores
% Insectivores
3
3
23.7%
13.5
71.2%
3
3
5
5
5
                % Pioneer Species
                % Carnivores
Catch per unit effort

% Simple lithophils

% DELT anomalies
13.5%


156

5.1%

0

Total  IBI Score
                                                                    46
                                                32

-------
                                                                        Middle Fork Anderson River
Site 6 (Hottell  1980)
Perry Co: Middle Fork Anderson River, at SR 145 bridge, Bristow, Clark Twp. T 4S R 3W S 27
Drainage area: 39.8 mi2
Metric
Category
Metric
                                    Scoring Criteria
Actual
IBI Score
Species
Composition
Fish
Condition
Total number of species

Number of darter/sculpin/
madtom species
Number of darter  species

% Headwater species
Number of sunfish species

Number of minnow species
27



Trophic
Composition

Number of sucker species
Number of sensitive species
% Tolerant species
% Omnivores
% Insectivores
4
4
36.7%
31.5%
58.9%
5
3
3
3
5
                % Pioneer  Species
                % Carnivores
Catch per unit effort

% Simple lithophils

% DELT anomalies
 2.1%                  3


333                    5

7.5%                   1

0                      5

Total  IBI Score         44
                                                33

-------
 Biological Characterization
Site M6 (Stefanavage  1989)
Perry Co: Middle Fork Anderson  River, at SR  145 bridge, Bristow, Clark Twp. T 4S R 3W S 27
Drainage area:  39.8 mi2
Metric
Category
 Metric
                                     Scoring Criteria
 Actual
                                                      IBI Score
Species
Composition
Fish
Condition
Total number  of species

Number of darter/sculpin/
madtom species
Number of darter species

% Headwater  species
Number of sunfish species

Number of minnow species
 23



Trophic
Composition

Number of sucker species
Number of sensitive species
% Tolerant species
% Omnivores
% Insectivores
4
3
29.6 %
10.2%
62.4%
5
3
3
5
5
                % Pioneer Species
                % Carnivores
Catch per unit effort

% Simple lithophils

% DELT anomalies
 8.1%


186

7.0%

0

Total IBI Score
                                                                    44
                                                34

-------
                                                                         Middle Fork Anderson River
Site 7 (Keller 1970)
Perry Co: Sulphur Fork, 1.5 mi W SR 145 off dirt road, 3 mi SE Bristow, Anderson  Twp. T 5S R 3W S 11
Drainage area: ca. 23.2 mi2
Metric
Category
                                    Scoring Criteria
Metric
Actual
IBI Score
Species
Composition   Total number  of species

               Number  of darter/sculpin/
               madtom  species
               Number  of darter species

                % Headwater  species
               Number  of sunfish species

               Number  of minnow species
               Number  of sucker species

               Number  of sensitive species

                % Tolerant  species

Trophic
Composition    % Omnivores

                % Insectivores

                % Pioneer Species
                % Carnivores
                               17
 Fish
 Condition
Catch per unit effort

 % Simple lithophils

 % DELT anomalies
4
1
30.1%
23.1%
62.4%
5
1
3
3
5
7.5%                  5


173                    3

2.3%                  1

0                      5

Total IBI Score         44
                                                  35

-------
Biological Characterization
Site 7 (Hottell 1980)
Perry Co: Sulphur Fork, 1.5 mi W SR 145 off dirt road, 3 mi SE Bristow, Anderson Twp. T 5S R 3W S 12
Drainage area: 23.2 mi2
Metric
Category
Species
Composition
Metric
Total number of species
Scoring Criteria
Actual
25

IBI Score
5
Fish
Condition
                Number of darter/sculpin/
                madtom species
                Number of darter species

                % Headwater species
                Number of sunfish species

                Number of minnow species



Trophic
Composition

Number of sucker species
Number of sensitive species
% Tolerant species
% Omnivores
% Insectivores
3
4
23.4%
21.7%
54.9%
3
3
5
3
5
                % Pioneer Species
                % Carnivores
Catch per unit effort

% Simple lithophils

% DELT anomalies
2.9%


410

7.1%

0

Total IBI Score
                                                                     46
                                                 36

-------
                                                                        Middle Fork Anderson River
Site M7 (Stefanavage 1989)
Perry Co: Sulphur Fork, 1.5 mi W SR 145 off dirt road, 3 mi SE Bristow, Anderson Twp. T 5S R 3W S 12
Drainage area: 23.2 mi2
Metric
Category
Metric
                                    Scoring Criteria
Actual
mi Score
Species
Composition
Total number of species

Number of darter/sculpin/
madtom species
Number of darter  species

% Headwater species
Number of sunfish species

Number of minnow species
12

Number of sucker species
1
Number of sensitive species 2

Trophic
Composition


Fish
Condition


% Tolerant species
% Omnivores
% Insectivores
% Pioneer Species
% Carnivores

Catch per unit effort
% Simple lithophils
% DELT anomalies
28.7%
26.3%
65.1%
7.8%

129
3.1%
0
Total IBI Score
3
1
3
3
5
5

1
1
5
34
                                                37

-------
Biological Characterization
Site 92131 (Simon 1992)
Perry Co: Kraus  Creek, at SR 145 bridge, 9 mi N Cannelton, Anderson Twp. T 5S R 3W S 26
Drainage area: 6.2 mi2
Metric
Category
Metric
                                    Scoring Criteria
Actual
IBI Score
Species
Composition
Total number of species          17

Number of darter/sculpin/
madtom species                 2
Number of darter species

% Headwater species            2.5%
Number of sunfish species

Number of minnow species       6
Number of sucker species
Number of sensitive species 3

Trophic
Composition


Fish
Condition


% Tolerant species
% Omnivores
% Insectivores
% Pioneer Species
% Carnivores
Catch per unit effort
% Simple lithophils
% DELT anomalies
20.9%
18.3%
59.7%
50.7%
278
7.2%
0
Total IBI Score
3
5
3
5
1
5
1
5
40
                                                38

-------
                                                                         Middle Fork Anderson River
Site 92132 (Simon  1992)
Perry Co: Sulphur  Fork, at SR 145 bridge, 2.5 mi S Bristow, Anderson Twp. T 5S R 3W S 11
Drainage area: 27.1 mi2
Metric
Category
Metric
                                    Scoring Criteria
Actual
IBI Score
Species
Composition   Total number  of species

               Number of darter/sculpin/
               madtom species
               Number of darter species

                % Headwater  species
               Number of sunfish species

               Number of minnow species
               Number of sucker species

               Number of sensitive species

                % Tolerant species

Trophic
Composition    % Omnivores

                % Insectivores

                % Pioneer Species
                % Carnivores
Fish
Condition
Catch per unit effort

% Simple lithophils

% DELT anomalies
                               16
1
2
39.2%
16.3%
61.4%
3
I
3
5
5
2.6%


153

0%

0

Total  IBI Score
                                                                     36
                                                 39

-------
Biological Characterization
Site 92133 (Simon 1992)
Perry Co: Middle Fork Anderson River, at dirt
23
Drainage area:
Metric
Category
Species
Composition





Trophic
Composition


Fish
Condition


41.8mi2
Metric
Total number of species
Number of darter/sculpin/
madtom species
Number of darter species
% Headwater species
Number of sunfish species
Number of minnow species
Number of sucker species
Number of sensitive species
% Tolerant species
% Omnivores
% Insectivores
% Pioneer Species
% Carnivores

Catch per unit effort
% Simple lithophils
% DELT anomalies
road bridge, 0.75 mi NE Bristow, Clark Twp. T 4S R 3W S

Scoriae Criteria
Actual
23
3
5
2
3
37.3%
36.7%
47.6%
5.8%

311
4.2%
0
Total IBI Score


IBI Score
5
3
5
3
3
3
1
3
5

5
1
5
47
                                                  40

-------
                                                                       Middle Fork Anderson River
Site 92134 (Simon 1992)
Perry Co: Middle Fork Anderson  River, at SR 62 bridge, 1 mi E Uniontown, Oil Twp. T 3S R 2W S 28
Drainage area: 8.18 mi2
Metric
Category
Metric
                                   Scoring Criteria
Actual
IBI Score
Species
Composition
Total number of species         14

Number of darter/sculpin/
madtom species                3
Number of darter species

% Headwater species           29.2%
Number of sunfish species

Number of minnow species      3
Number of sucker species
Number of sensitive species 1

Trophic
Composition


Fish
Condition


% Tolerant species
% Omnivores
% Insectivores
% Pioneer Species
% Carnivores

Catch per unit effort
% Simple lithophils
% DELT anomalies
6.9%
5.5%
56.9%
36.1%

72
11.1%
0
Total IBI Score
1
5
5
5
3

1
1
5
39
                                                41

-------
Biological Characterization
Appendix II. Statewide list of Indiana  species considered  to be headwater species for evaluating permanent
habitat in headwater streams (after Smith 1971).
Least brook lamprey

American brook lamprey

Redside  dace

Blacknose dace

Southern redbelly dace

Brook stickleback

Fantail darter

Mottled  sculpin

Banded sculpin
Lampetra aepyptera

Lampetra appendix

Clinostomus elongatus

Rhinichthys atratulus

Phoxinus erythrogaster

Culaea inconstans

Etheosloma flabellare

Cottus bairdi

Cottus carolinae
                                                   42

-------
                                                                           Middle Fork Anderson River
Appendix III. Statewide list of Indiana fish species considered to be sensitive to a wide variety of
environmental disturbances including water quality and habitat degradation.
Common Name         Scientific Name
Ohio lamprey           Ichthyomyzon bdellium
Northern brook lamprey I.fossor
Least brook lamprey     Lampetra aepyptera
American brook lamprey L. appendix
Paddlefish

Goldeye
Mooneye

Redside dace
Streamline  chub
Gravel chub
Speckled chub
Bigeye chub
Pallid shiner
Rosefui shiner
Horayhead chub
River chub
Pugnose  shiner
Popeye shiner
Bigeye shiner
Ironcolor shiner
Blacknose shiner
Blackchin shiner
Sand shiner
Silver shiner
Rosyface shiner
Silverband shiner
Weed  shiner
Mimic shiner
Channel  shiner
Pugnose minnow
Longnose dace

Blue sucker
Highfm  carpsucker
Northern hogsucker
Silver redhorse
Ohio redhorse
River redhorse
 Black redhorse
 Golden  redhorse
Polyodon spatula

Hiodon alosoides
H. tergisus

Clinostomus elongatus
Erimystax dissimilis
E. x-punctatcf
Extrarius aestivalis
Hybopsis amblops
H. amnis
Lythrurus ardent
Nocomis biguttatus
N. micropogon
Notropis anogenus
N. ariommus
N. hoops
N. chafybaeus
N. heterolepis
N. heterodon
N. ludibunduf
N. photogenis
N. rubellus
N. shumardi
N. texanus
N. volucellus
N. wickliffi
Opsopoeodus  emiliae
Rhinichthys cataractae

Cydeptus elongatus
Carpiodes velifer
Hypentelium nigricans
Moxostoma anisurum
Moxostoma breviceps
Moxostoma carinatum
M. duquesnei
M. erythrurum
Common Name
 Shorthead  redhorse
 Greater redhorse

 Mountain madtotn
 Slender madtom
 Stonecat
 Brindled madtom
 Freckled madtom
 Northern  madtom

 Northern  cavefish
 Southern  cavefish

 Northern  studfish
 Starhead topminnow

 Brook silverside

 Rock bass
 Longear sunfish
 Smallmouth bass

 Western sand darter
 Eastern sand darter
 Crystal darter
 Greenside darter
 Rainbow  darter
 Bluebreast darter
 Harlequin darter
 Spotted darter
 Spottail darter
 Tippecanoe darter
 Variegate  darter
 Banded darter
 Logperch
 Channel darter
 Gilt darter
 Slenderhead  darter
 Dusky darter
 Saddleback darter
Scientific Name
M. macrolepidotum
M. Valenciennes i

Noturus eleutherus
N. exilis
N.flavus
N. miurus
N. noaurnus
N. stigmosus

Amblyopsis spelaea
Typhlichthys subterraneus

Fundulus  catenatus
F. dispar

Labidesthes  sicculus

Ambloplites  rupestris
Lepomis megalotis
Micropterus dolomieu

Ammocrypta  clara
A. pellucida
Crystallariaasprella
Etheostoma  blennioides
E. caeruleum
£'. camurum
E. histrio
E. maculatum
E. squamiceps
E. tippecanoe
E. variatum
E. zonale
Percina caprodes
P. copelandi
P. evides
P. phoxocephala
P. sciera
P. vigil
 * This species is tentatively placed in this category
   until further life history data is available.
                                                   43

-------
 Biological Characterization
 Appendix IV. Statewide list of species considered to be highly tolerant  to a wide variety of environmental
 disturbances including water quality and habitat degradation.
 Common Name
 Longnose gar
 Shortnose gar
 American eel
 Gizzard  shad
 Central mudminnow
 Goldfish
 Grass  carp
 Red shiner
 Carp
 Silver  carp
 Bighead  carp
 Black  carp
 Golden shiner
 Bluntnose minnow
 Fathead  minnow
 Blacknose dace
 Creek  chub
 Rudd
 White  sucker
 River carpsucker
 Quillback
 Smallmouth buffalo
 Bigmouth buffalo
 Black bullhead
 Yellow bullhead
 Channel  catfish
 Flathead  catfish
 Banded killifisfa
White  bass
White  perch
Green  sunfish
Freshwater drum
 Scientific Name
 Lepisosteus osseus1
 Lepisosteus platostomus'
 Anguilla rostrata
 Dorosoma cepedianum
 Umbra limi
 Carassius aural us
 Ctenopharyngodon idella
 Cyprinella lutrensis
 Cyprinus carpio
 Hypophthalmichthys molitrix
 H. nobilis
 Mylopharyngodon piceus
 Notemigonus crysoleucas
 Pimephales  notatus
 Pimephales promelas
 Rhinichthys  atratulus
 Semotilus atromaculatus
 Scardinius erythrophthalmus
 Catostomus  commersoni
 Carpiodes carpio1
 Carpiodes cyprinus*
 Ictiobus bubalu?
 Ictiobus cyprineUus*
Ameiurus  melas
Ameiurus  natalis*
Ictalurus punctatus
Pylodictis olivaris
Fundulus diaphanus
Morone chrysops'
Morone americana
Lepomis cyanellus
Aplodinotus  grunniens'
" This species is being considered as
 listed in Simon (1991; 1992).
   'highlytolerant" rather than  "moderately tolerant" or "unclassified" as
                                                  44

-------
                                                                          Middle Fork Anderson River
Appendix V. Statewide list of Indiana  fish species considered to be omnivores.
Common Name                        Scientific Name

Gizzard shad                           Dorosoma cepedianum*
Threadfin shad                         Dorosoma petenense'

Central  mudminnow                    Umbra limi

Goldfish                               Carassius auratus
Grass carp                             Ctenopharyngodon idella
Carp                                   Cyprinus carplo
Cypress minnow                        Hybognathus hayi
Mississippi silvery minnow               Hybognathus nuchalis
Silver carp                             Hypopthalmichthys molitrix
Black carp                             Mylopharyngodon piceus
Bluntnose minnow                      Pimephales not at us
Fathead minnow                        Pimephales promelas
Bullhead minnow                       Pimephales vigilax
Rudd                                  Scardinius erythrophthalmus

River carpsucker                        Carpiodes carpio*
Quillback                              Carpiodes cyprinus*
Highfin carpsucker                      Carpiodes velifer
White sucker                           Catostomus commersoni
  This species is being added to the Statewide list based on additional information not available in Simon
  (1991).
                                                  45

-------
 Biological Characterization
Appendix VI. Statewide list of Indiana fish species considered to be insectivores.
Common Name
Shovelnose sturgeon

Goldeye
Mooneye

Redside  dace
Lake chub
Red shiner
Spotfin shiner
Steelcolor  shiner
Silverjaw minnow
Streamline chub
Gravel chub
Speckled chub
Bigeye chub
Pallid shiner
Striped shiner
Common  shiner
Rosefin shiner
Ribbon shiner
Redfin shiner
Silver chub
Hornyhead  chub
River chub
Golden shiner
Pugnose shiner
Emerald  shiner
Popeye shiner
River shiner
Bigeye shiner
Ghost shiner
Ironcolor shiner
Bigmouth shiner
Blacknose  shiner
Blackchin shiner
Spottail shiner
Sand shiner
Silver shiner
Rosyface shiner
Silverband  shiner
Weed shiner
Mimic shiner
Channel shiner
 Scientific Name
 Scaphirhynchus platorynchus

 Hiodon alosoides
 H. tergisus

 Clinostomus elongatus
 Couesius plumbeus
 Cyprinella lutrensis
 C. spiloptera
 C. whipplei
 Ericymba buccata
 Erimystax dissimilis
 E. x-punctata
 Extrarius aestivalis
 Hybopsis amblops
 H. amnis
 Luxilus chrysocephalus
 L. cornutus
 Lythrurus ardens
 L. fumeus
 L. umbratilis
 Macrhybopsis storeriana
 Nocomis biguttatus
 N. micropogon
 Notemigonus crysoleucas
 Notropis anogenus
 N. atherinoid.es
 N. ariommus
 N. blennius
 N. hoops
 N. buchanani
 N. chalybaeus
 N. dorsalis
 N. heterolepis
 N. heterodon
 N. hudsonius
 N. ludibundus
N. photogenis
N. rubellus
N. shumardi
N. texanus
N. volucellus
N. wickliffi
Common Name
 Pugnose minnow
 Suckermouth  minnow
 Longnose dace

 Blue sucker
 Longnose sucker
 Creek chubsucker
 Lake chubsucker
 Northern hogsucker
 Spotted sucker
 Silver redhorse
 Ohio redhorse
 River redhorse
 Black redhorse
 Golden redhorse
 Shorthead redhorse
 Greater redhorse

 Black bullhead
 Yellow bullhead
 Brown bullhead
 Mountain madtom
Slender madtom
Stonecat
Tadpole madtom
Brindled  madtom
Freckled  madtom
Northern madtom

Pirate perch

Trout-perch

Northern studfish
Banded killifish
Starhead  topminnow
Blackstripe topminnow

Mosquitofish

Brook silverside
Inland silverside
 Scientific Name
 Opsopoeodus emiliae
 Phenacobius mirabilis
 Rhinichthys cataractae

 Cycleptus elongatus
 Catostomus catostomus
 Erimyzon oblongus
 E. sucetta
 Hypentelium nigricans
 Minytrema melanops
 Moxostoma anisurum
 M. breviceps
 M. carinatum
 M. duquesnei
 M. erythrurum
 M. macrolepidotum
 M. valenciennesi

 Ameiurus melas
 A.  natalis
 A.  nebulosus
 Noturus eleutherus
 N.  exilis
 N. flavus
 N.  gyrinus
 N.  miurus
 N.  nocturnus
 N.  stigmosus

 Aphredoderus sayanus

 Percopsis omiscomaycus

 Fundulus  catenatus
 F. diaphanus
 F. dispar
 F. notatus

 Gambusia affinis

Labidethes sicculus
Menidia beryllina
                                                  46

-------
                                                                            Middle Fork Anderson River
Common Name
Brook stickleback
Ninespine stickleback
Threespine stickleback

Flier
Green sunfish
Pumpkinseed
Orangespotted  sunfish
Bluegill
Longear  sunfish
Redear sunfish
Spotted sunfish
Bantam sunfish
Scientific Name
Culaea inconstans
Pungitius pungitius
Gasterosteus aculeatus

Centrarchus macropterus
Lepomis  cyanellus
L. gibbosus
L. humilis
L. macrochirus
L. megalotis
L. microlophus
L. punctatus
L. symmetricus
Common Name
Mottled sculpin
Banded sculpin
Spoonhead sculpin

Round goby
Scientific Name
Cottus bairdi
C. carolinae
C. ricei

Neogobius melanostomus
' This list of species  is based on best  available
 information.  Species tentatively placed  in this
 category will remain until additional information is
 available.
Banded pygmy sunfish   Elassoma zonatum
Crystal darter
Western  sand darter
Eastern  sand darter
Mud darter
Greenside  darter
Rainbow darter
Bluebreast  darter
Bluntnose  darter
Iowa darter
fantail darter
Slough darter
Harlequin  darter
Spotted darter
Least darter
Johnny darter
Orangethroat  darter
Spottail darter
Tippecanoe darter
Variegate  darter
Banded darter
Logperch
Channel  darter
Gilt darter
Blackside darter
Slenderhead  darter
Dusky darter
River darter
Yellow saddleback
darter
Crystallaria asprella
Ammocrypta  clara
A. pellucida
Etheostoma asprigene
E. blennioides
E. caeruleum
E. camurum
E. chlorosoma
E. exile
E. flabellare
E. gracile
E. histrio
E. maculatum
E. microperca
E. nigrum
E. spectabile
E. squamiceps
E. tippecanoe
E. variatum
E. zonale
Percina caprodes
P. copelandi
P. evides
P. maculata
P. phoxocephala
P. sciera
P. shumardi

P. vigil
                                                   47

-------
 Biological Characterization
 Appendix VII. Statewide list of Indiana  fish species considered to be carnivores.
 Common Name
 Ohio lamprey
 Chestnut  lamprey
 Silver lamprey
 Sea lamprey

 Alligator  gar
 Spotted gar
 Longnose gar
 Shortnose gar

 Bowfin

 American eel

 Skipjack herring

 Rainbow  trout
 Coho salmon
 Chinook salmon
 Brook trout
 Lake trout
 Atlantic salmon
 Brown trout

 Grass pickerel
 Northern  pike
 Muskellunge

 Blue catfish
 Channel catfish
 Flathead catfish

 White bass
 Yellow bass
 Striped bass

 Rock bass
 Warmouth
 Smallmouth  bass
 Spotted bass
 Largemouth  bass

Walleye
Sauger
 Scientific Name
 Ichthyomyzon bdellium
 I. casteneus
 I. unicuspis
 Petromyzon marinus

 Atractosteus spatula
 Lepisosteus oculatus
 L. osseus
 L. platostomus

 Amia calva

 Anguilla rostrata

 Alosa chtysochloris

 Oncorhynchus mykiss
 O. kisutch
 O. tshawytscha
 Salvelinus fontinalis
 S. namaycush
 Salmo solar
 S. trutta

 Esox americanus
 E. lucius
 E. masquinongy

 Ictalurus furcatus
 I. punctatus
 Pylodictis olivaris

 Morone chrysops
 M. mississippiensis
 M. saxatilis

Ambloplites rupestris
Lepomis gulosus
Micropterus dolomieu
M. punctulatus
M. salmoides

Stizostedion vitreum
S. canadense
                                                  48

-------
                                                                          Middle Fork Anderson River
Appendix VIII. Statewide list of Indiana  species considered to be pioneer species (indicators of temporally
unavailable or stressed  habitats (after Larimore  and Smith  1963; Smith 1971)).
Common Name

Central stoneroller

Largescale stoneroller

Silverjaw minnow

Bluntnose  minnow

Fathead minnow

Creek chub

Creek chubsucker

Lake chubsucker

Green sunfish

Johnny darter

Orangethroat darter
Scientific Name

Campostoma  anomalum

Campostoma  oligolepis

Ericymba buccata

Pimephales notatus

        Pimpehales promelas

Semotilus atromaculatus

Erimyzon oblongus

Erimyzon sucetta

Lepomis cyanellus

Etheostoma nigrum

Etheostoma spectabile
                                                  49

-------
Biological Characterization
Appendix IX. Statewide  list of Indiana fish species considered to be simple iithophilous spawners.
Common Name
Paddlefish
Lake sturgeon
Shovelnose sturgeon

Redside dace
Lake chub
Streamline chub
Gravel chub
Cypress minnow
Miss, silvery minnow
Bigeye chub
Pallid shiner
Popeye shiner
River shiner
Bigeye shiner
Silver shiner
Rosyface shiner
Silverband shiner
Suckermouth minnow
Southern redbelly dace
Blacknose dace
Longnose dace

Blue sucker
Longnose sucker
White sucker
Northern hogsucker
Scientific Narne
Polyodon spathula
Acipenser Julvescens
Scaphirhynchus platorynchus

Clinostomus elongatus
Couesius plumbeus
Erimystax dissimilis?
E. x-punctatct
Hybognathus hayi*
H. nuchalis
Hybopsis amblops
H. (minis'
Notropis ariommus*
N. blennius
N. hoops
N. photogenis
N. rubellus
N. shumardi
Phenacobius mirabilis
Phoxinus erythrogaster
Rhinichthys atratulus
R. cataractae

Cycleptus elongatus
Catostomus catostomus
C. commersoni
Hypentelium nigricans
Common Name

 Spotted  sucker
 Silver redhorse
 Ohio redhorse
 River redhorse
 Black redhorse
 Golden redhorse
 Shorthead  redhorse
 Greater  redhorse

 Burbot

 Western sand darter
 Eastern  sand  darter
 Crystal  darter
 Rainbow darter
 Orangethroat  darter
 Tippecanoe darter
 Variegate darter
 Logperch
 Channel darter
 Gilt darter
 Blackside darter
 Slenderhead darter
 Dusky darter
 River darter
 Saddleback darter
 Sauger
Walleye
 Scientific Name

 Minytrema melanops
 Moxostoma anisurum
 M. breviceps
 M. carinatum
 M. duquesnei
 M. erythrurum
 M. macrolepidotum
 M. valenciennesi

 Lota  lota

 Ammocrypta clara
 A. pellucida
 Crystallaria asprella
 Etheostoma caeruleum
 E. spectabile
 E. tippecanoe
 E. variatum
 Percina caprodes
 P. copelandi
 P. evides
 P. maculata
P. phoxocephala
P. sciera
P. shumardi
P. vigil
Stizostedion canadense
S. vitreum
                                                     * This species is tentatively placed in this
                                                      classification until further life history information is
                                                      available.
                                                  50

-------