United States
        Environmental Protection
        Agency
Region 5
77 West Jackson Boulevard
Chicago, Illinois 60604
EPA-905-R-92-007
December 1994
$EPA  Development of Index of
        Biotic Integrity Expectations
        For the Ecoregions of Indiana
        II. Huron-Erie Lake Plain

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                                                     EPA 905/R-92/007
Development of Index of Biotic Integrity Expectations

for the Ecoregions of Indiana: II. Huron-Erie Lake Plain
                     Thomas P. Simon
            U.S. Environmental Protection Agency
             Standards and Assessment Section
                77 West Jackson, WQS-16J
                    Chicago. IL 60604
                    In cooperation with:
       Indiana Department of Environmental Management
        Water Quality Surveillance and Standards Branch
            100 North Senate Ave., P.O. Box 6015
                Indianapolis, IN 46206-6015
                      March 9, 1994

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                                               NOTICE

Use of this document  is intended  for the objective facilitation of information exchange  between the States
and  Federal  Water  Pollution Control Biologists for which  it was  intended.   Mention of trade names  or
commercial  products  does  not constitute  endorsement  or recommendation  for use.
 When citing this document:

 T.P. Simon.  1994. Development of Index of Biotic Integrity expectations  for the Ecoregions  of Indiana. II.
 Huron-Erie Lake Plain. U.S. Environmental Protection Agency, Region V,Water Division,Watershed and Non-
 Point  Source Branch, Chicago,  IL. EPA 905/R-92/007.


 If requesting  copies  of this document:

 U.S. Environmental  Protection  Agency
 Publication  Distribution Center, DDD
 11027 Kenwood  Road,  Bldg. 5 - Dock 63
 Cincinnati, OH  45242

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                                    TABLE OF CONTENTS
Section	                                                Page
i.  List of Figures                                                                        iii
ii. List of Tables                                                                        v
iii. Executive Summary                                                                   vii
iv. Acknowledgements                                                                    ix

1.0 INTRODUCTION                                                                    1

   Definition of Reference Conditions                                                       1
   Criteria for Selecting  Reference  Sites                                                    2
2.0 STUDY AREA                                                                       3

   Physiographic Provinces                                                                3
   Ecoregions                                                                            5
       Eastern  Corn Belt Plain                                                            5
       Huron-Erie Lake Plain                                                             5
   Natural Areas                                                                         7
   Drainage Features                                                                     9
       Maumee River                                                                    9
       St.  Joseph  River                                                                  9
       St.  Marys River                                                                  10
   Historical Maumee River data                                                          10

3.0 MATERIALS AND  METHODS                                                      10

   Sampling                                                                            10
       Site Specific                                                                     10
       Habitat                                                                          11
   Community Analysis                                                                  11
       Sampling Considerations                                                           12
       Sample  Site Selection                                                             12
       Index  of Biotic Integrity                                                           14
   Metrics                                                                              14
   Scoring  Modifications                                                                 49
4.0 RESULTS AND DISCUSSION                                                        50

   Maumee River Drainage                                                               SO
   St. Joseph  River Drainage                                                             54
   St. Mary's River Drainage                                                              55
   Reference  Sites                                                                      56
   Biocriteria  Comparison of the Huron-Erie Lake Plain                                      58

6.0 LITERATURE CITED                                                               61

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7.0 APPENDICES

   A.  Tolerance, trophic, and reproductive guild classifications for computing the Index of Biotic
       Integrity for Indiana taxa.
   B.  Metric specific Index of Biotic Integrity scores for sites in the Maumee River  drainage.
   C.  Fish nomenclature changes for the species offish occurring within the political boundaries of
       Indiana.

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                                          LIST  OF FIGURES

Figure
Number	                     Page

1       Map of Indiana showing the major and minor drainage  basins
        (after USGS  drainage  maps).                                                             4

2       Map of Indiana showing the ecoregions   designation
        of Omernik  and Gallant (1988)                                                          6

3       Map of Indiana indicating  the natural  areas  designation
        ofHomoya  et al. (1985).                                                                8

4       Maumee River drainage indicating the location of sampled locations
        during 1991.                                                                           12

5       Maximum species  richness lines for determining  trends in total
        number of species  with increasing  drainage area for the Maumee
        River drainage.                                                                         19

6       Maximum species  richness lines for determining  trends in number
        of darter/madtom/sculpin   species  with increasing drainage area for
        the Maumee  River drainage.                                                             22

7       Maximum species  richness lines for determining  trends in number
        of sunfish species  with increasing  drainage  area  for the Maumee
        River drainage.                                                                         24

8       Maximum species  richness lines for determining  trends in number  of
        minnow species with increasing  drainage  area  for the  Maumee  River
        drainage.                                                                               27

9       Maximum species  richness lines for determining  trends in number
        of sucker  species  with increasing  drainage area for the Maumee
        River drainage.                                                                         28

10      Maximum species  richness lines for determining  trends in number
        of sensitive  species  with increasing  drainage  area for the
        Maumee River drainage.                                                                31

11      Maximum species  richness lines for determining  trends in the
        proportion  of tolerant  species  with increasing  drainage  area for
        the Maumee  River drainage.                                                             32

12      Maximum species  richness lines for determining  trends in the
        proportion  of omnivores with increasing  drainage area for the
        Maumee River drainage.                                                                36
                                                  in

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                                  LIST OF  FIGURES (CONTINUED)

Figure
Number	                     page

13      Maximum species  richness  lines for determining  trends in the
        proportion  of insectivores with increasing  drainage  area  for the
        Maumee River drainage.                                                                3g

14      Maximum species  richness  lines for determining  trends in the
        proportion  of pioneer species  with increasing drainage area  for the
        Maumee River drainage.                                                                40

15      Maximum species  richness  lines for determining  trends in the
        proportion  of carnivores with increasing  drainage area for the
        Maumee River drainage.                                                                4 j

16      Maximum species  richness  lines for determining  trends in the
        catch  per unit effort with increasing  drainage  area for the
        Maumee River drainage.                                                                43

17      Maximum species  richness  lines for determining  trends in the
        proportion  of simple litbophil  species with increasing  drainage
        area  for the Maumee  River drainage.                                                    45

18      Maximum species  richness  lines for determining  trends in the
        proportion  of diseased,  eroded  fins, lesions, and  rumors  (DELT)
        with increasing  drainage area  for the Maumee River drainage.                             48
                                                  IV

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                                           LIST OF  TABLES
Table
Number	Page

1       Attributes of Index of Biotic Integrity (IBI) classification,
        total IBI scores,  and integrity classes  from Karr et al. (1986).                             16

2       Index of Biotic Integrity metrics used  to evaluate headwater  streams
        (^20 miles2 drainage  area)  sites  in the Maumee River drainage                           17

3       Index of Biotic Integrity metrics used  to evaluate wadable  river
        (> 20-1,000 miles2 drainage  area) sites  in the Maumee  River drainage.                    18

4       The distributional characteristics  of Indiana darter (Etheostomatini),
        madtom (Noturus),  and sculpin (Cottus) species.                                         23

5       List of Indiana sunfish  species  for evaluating  quality pool habitats.                         25

6       Distributional characteristics  of Indiana sucker  species (family
        Catostomidae)  in the Maumee River drainage.                                            28

7       List of Indiana fish species  considered  to  be  sensitive  to a wide
        variety  of environmental disturbances  including  water quality and
        habitat  degradation.                                                                       32

8       List of Indiana fish species  considered  to  be  highly tolerant to
        a wide  variety of environmental  disturbances  including water
        quality  and  habitat degradation  for headwater  and wadable river sites.                    35

9       List of Indiana fish species  considered  to  be  omnivores.                                   37

10      List of Indiana fish species  considered  to  be  indicator"  ->f temporally
        unavailaole  or stressed  habitats (Larimore and  Smith 1963; Smith 1971)                  41

11      List of Indiana species  considered  to  be simple lithophilic
        spawners.                                                                                47

12      Species  list of taxa collected  in the  Maumee  River drainage:  Maumee
        St.  Mary's,  and St. Joseph  River drainages, Indiana, during 1991.                         53

13      Reference  sites determined  using  fish community biotic  integrity for the
        Maumee River drainage,  Indiana.                                                         59

14      Comparison  of Ohio Environmental  Protection  Agency (1989) reference conditions
        derived from the Stream  Regionalization Project with reference conditions
        developed  from Indiana portions of the Huron-Erie  Lake Plain (Ohio EPA 1989).           62

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                                       EXECUTIVE  SUMMARY

The Clean Water Act Amendments  of 1987 suggest  the development  of biological criteria for evaluating
the quality of the nation's surface waters.  The Maumee  River drainage  was investigated  in Indiana to
determine  water resource expectations  for the Huron-Erie Lake Plain. A total of 77 sites were sampled  in
the Maumee River drainage in order  to develop and calibrate an Index  of Biotic Integrity for  use  in this
region of Indiana.  The Maumee  basin  crosses  two ecoregions,  based  on anticipated  variance, sub-
drainages  were established  using natural divisions as  recognized  by Homoya et al. (1985).

Three  sub-drainages   are recognized  and include the major drainage units of the Maumee River:  Maumee
River. St. Joseph  River, and St. Mary's River drainages.  Graphical  analysis  of the data enabled  the
construction  of maximum species  richness  lines for calibrating the  Index of Biotic Integrity for 12 metrics
as modified  for application to headwater  and  mid-sized wadable  rivers in Indiana.  Metrics were primarily
based  on the previous works  of Karr (1981),  Karr et al. (1986), Ohio EPA (1987), and  Simon  (1991).
Metrics are similar to those  developed  for the Lake Michigan Division of the Central Corn Belt Plain. This
includes  the number  of minnow  and  sun fish species,  proportion  of pioneer species,  and  a combination
of sensitive benthic  insectivores,  e.g. darters,  madtoms,  and sculpins.

Separate   metrics  were developed for headwater streams  (< 20 miles2) and wadable river (20-1000
miles2) drainage  area.  Scoring  criteria modifications  were instituted  when less than 50 individuals  were
collected  from a sampling  location. This affected the trophic composition, tolerance,  simple lithophil, and
disease proportional  metrics.  Stations with drainage  areas  less than 20 miles2 used  a  metric  which
included  darters,  madtoms,  and  sculpins  (all benthic  insectivores).   These species are sensitive
indicators  of a high quality aquatic  resource.  In reaches  with drainage  areas  greater than  20  miles2 a
metric evaluating  only darter species  was  used  following the original IBI. The proportion  of pioneer
species was substituted  for the  proportion of carnivores  in small  headwater  streams.  The number  of
sunfish species  was  retained for both categories of stream sizes.  Indiana numerical criteria from the
Huron-Erie Lake Plain is comparable  to Ohio's statewide numerical  expectations.

The distribution of IBI scores represented   a normal curve for the Maumee and St. Joseph  River
subdrainages.   Declining biological  integrity was observed  in the headwaters  of the St. Mary's River
subdrainage.  This seemed  to be the  pattern  for almost the entire Maumee River drainage.  The trend was
towards  increasing  biological  integrity with increasing  drainage area in all subdrainages.   The only
exception  was  the St. Joseph  River which possessed   considerably   better fish community  at the
headwaters.  A comparison  of Huron-Erie Lake Plain numerical  biocriteria was  made  between  reference
condition  expectations  for Indiana with statewide  criteria for  Ohio. Most metrics  were  statistically
comparable   including the number of species,   number  of minnow species,  number of sucker  species,
proportion  of tolerant species,  proportion of carnivores, proportion   of simple  lithophils, and proportion  of
disease,  eroded  fins, lesions, and tumors  (DELT). Metrics which were significantly different included
proportion  of omnivores  and proportion  of insectivores which are  more  stringent  in Indiana criteria.
However, six other metrics had  more stringent  expectations   in Ohio's statewide  numerical criteria than
was observed  in the Indiana portion  of the Huron-Erie Lake Plain. Significantly different metrics included
number of species  in wadable rivers, number   of darter species, proportion of tolerant  species   in
headwater  streams,  number of sensitive species, proportion  of omnivores, proportion  of insectivores,
proportion  of pioneer species,  and  proportion  of simple lithophils. Several plausible explanations   suggest
why these  differences exist. The first is that the sites in the Huron-Erie Lake  Plain are severely degraded
due  to the human modification of the landscape. A second explanation  is a reflection  of the historical
vicariant  events which occurred  post-glaciation.  A third explanation  is the statewide  criteria developed  in
Ohio should  have a greater  richness  and  composition  because  of the larger affected area.
                                                   vn

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                                       ACKNOWLEDGEMENTS

The U.S. Environmental  Protection Agency wishes to express  their appreciation  to those individuals
which enabled  this study to be completed:  Wayne Davis, Valerie Jones,  and Boniface Thayil, USEPA-
Region  V, Ambient Monitoring Section, and  John Winters, Dennis Clark, and Lee  Bridges, Indiana
Department  of Environmental Management  (IDEM) managed  and  facilitated logistics and sampling
needs.   Special  thanks  to Thomas  Lauer, Fisheries Scientist,  Indiana  Department  of Natural Resources
who  provided information from the Department's  stream  reports  which enabled  sampling at reference
sites.  Field assistance   was provided by Andrew Ellis, Doug Campbell,  and Gregory Nottingham,  Steve
Newhouse,   James  Stahl, and Stephen  Wente, IDEM biologists.  We express  our appreciation to all the
Indiana  landowners  which allowed access  across their property to facilitate River launching  of gear. We
are indebted to John Dustman, Indiana University-Northwest,  for use of work space to process  the large
volume  of samples.  Metric  expectation  graphs were  prepared  by Beth Simon. Hydrologic unit and
ecoregion  maps  were prepared by George Graettinger,  USEPA, Water Division, CIS Section, while the
Natural  Regions  of Indiana  map was prepared by Mary Van Buren, GIS Laboratory, School  of Public and
Environmental  Affairs,  Indiana University, Bloomington.  Shelby Gerking, Arizona State  University,
provided notes  and  copies  of valuable information from his previous  collection  efforts in Indiana.
Numerous  professional  courtesies  were provided by colleagues  which facilitated completion  of this
project:  Chris Yoder, Marc  Smith, and  Ed Rankin, Ohio EPA, provided  help in numerous  aspects  of this
study. Much information was gained  through conversations  with colleagues concerning  techniques  and
logistical aspects:  William Matthews, Brooks  Burr, Melvin Warren, Jr., Lawrence Page, John Lyons, Bob
Hughes, Phil Larsen, and Jim Omernik. Historic records were provided by Susan  Jewett, National
Museum of Natural  History; Douglas Nelson  and Gerald  Smith, University  of Michigan Museum of
Zoology; William Eschmeyer,  California Academy of Science;  and Ted Cavender,  The Ohio  State
University.  Special  thanks  to Wayne Davis, Lee Bridges, James  Stahl, Rhonda  Dufour, and  Steve
Newhouse   for constructive  review comments  on a previous draft of the  manuscript.  The project
manager,  chief scientist, and author  of this report was Thomas P. Simon,  Regional  Biocriteria
Coordinator.
Guest  Reviewers:

Mr. Chris Yoder, Ohio Environmental  Protection  Agency
Mr. Marc Smith, Ohio Environmental Protection  Agency
Dr. Lizhu Wang, Wisconsin  Department  of Natural Resources
                                                  IX

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                 Development   of  Index of Biotic  Integrity  Expectations
                 for the Ecoregions   of Indiana.  II. Huron-Erie  Lake  Plain
1.0 INTRODUCTION

The term  "biological  integrity" originated in the
Water  Pollution Control Act Amendments  of
1972 (PL 92-500) and has likewise appeared  in
subsequent  versions  (PL 95-217; PL 100-1). Karr
and Dudley (1981) defined biological integrity
as,  "the ability of an  aquatic  ecosystem  to
support and  maintain a balanced,  integrated,
adaptive  community  of organisms  having a
species composition,  diversity, and  functional
organization  comparable  to the best natural
habitats within a region". The use  of a biological
component  to evaluate  the  ambient  lotic aquatic
community  of our nations surface  waters has
been  well discussed  elsewhere (Karr et al.  1986;
Ohio  EPA 1987; Whittier et  al. 1987; Simon et
al. 1988;  Davis 1990; Fausch  et al.  1990; Karr
1991).

An assessment   of the Maumee River drainage
enabled  the  objective evaluation of specific
metrics performance   and  evaluation of reference
conditions for the  Huron-Erie Lake Plain. The
Maumee  River drainage has impacts associated
with channelization,  agriculture, and municipal
and point source  dischargers.  The primary point
sources  are  municipal facilities, chemical
manufacturers,  and electric  power generating
stations distributed in the main population
centers of the basin.  The  effects of
channelization  and agriculture  have been well
documented   (Raney  and  Menzel  1969; Brown
1976;  Brungs  and  Jones  1977; Hokanson  and
Biesinger 1980;  USEPA 1980; McCormick et  al.
1981;  EPRI  1981).

The objective  of this study  was to evaluate  the
biological integrity of Indiana water  resources
based  on "least impacted" reference  conditions
for establishing baseline conditions  (Hughes  et
al. 1986).  Least impacted  reference  sites  are
representative  of the subbasin  under study and
reflect the better  sites  with  minimum
anthropogenic   change.  Least impacted  is not
synonymous  with pristine.  Rather,  sites are
selected   for their representativeness   of the area.
The Maumee  River drainage and  the historical
Black Swamp  have been  dramatically  changed
over the last 250 years with the  draining of the
wetland and  the intensive  ditching projects  that
completely changed   the landscape.   The
following project  goals were addressed  during
the Huron-Erie Lake  Plain biological  criteria
project:

o Develop  biological  criteria for  headwater,
  mid-size,  and large  river reaches  using
  the Index of Biotic Integrity and habitat
  classification;

o Identify areas  of least disturbance  within
  the Huron-Erie Lake Plain for establishing
  reference condition;

o Develop  maximum  species  richness  (MSR)
  lines  from the reference   database  for each
  Index of Biotic Integrity metric  as a
  function of drainage  area;

o Compare  numerical biological  criteria
  expectations  between this study  and the
  State  of Ohio.

This technical  report  includes  specific  Index  of
Biotic  Integrity criteria including  the
development  of metrics and maximum species
richness  lines, to delineate  areas  of least
disturbance  in the Huron-Erie  Lake Plain. The
purpose  of this study  is not to verify ecoregion
boundaries  since additional  study  areas  would
need to be sampled   to determine the
heterogeneity  of the  "fuzzy border" areas. At the
termination  of the ecoregion effort a final report
will examine  the ecoregion  concept  for water
resource management  in  Indiana.
Definition of Reference  Conditions

In order  to make accurate evaluations  of the
biological condition  of the region,  various
baseline  geological,  geographic, and climatic
differences need to  be assessed.  The  goal is
not to provide a definition of pristine conditions,

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Indiana  Ecoregion
since these types of conditions  are either few in
number  or nonexistent  in heavily populated
states  (Hughes  et al. 1982; Whittier et al. 1987).
Our expectations  are based  on fish community
structure  and function to define attainable
natural  conditions of "least impacted"  or
reference conditions.  Assessment   of these
criteria need to be modified nationally, since
regional differences  can be attributed  to the
expectations  determining distribution  of fishes.
The ecoregion  concept   is useful for clustering
large homogeneous   regions,  since these  areas
are influenced  by different physical processes
(Omeraik 1987).

In order to select stations  for sampling  it is
necessary  to know  the  geographical  boundary
of the "ecoregions"  within the State of Indiana.  A
valid ecoregion  has boundaries  where
ecosystem  variables and patterns  emerge
(Hughes  et al. 1986). Omernik (1987) mapped
the ecoregions   of the conterminous United
States  from maps of land-surface  form, soil
types, potential natural  vegetation, and  land use.
Each ecoregion  was then  based  on areas  of
regional homogeneity.   Ecoregions  became  a
very useful mechanism   for determining
community complexity  and for establishing
boundaries  associated   with various land forms.

Ecoregions  provide a geographical  framework
for determining the appropriate  response  for
streams of similar proportion  and complexity.
Reference sites  are used for establishing  the
areas  of "least impact",  and will reveal the
current conditions of the surface  waters  of
Indiana. Once  ecoregional  expectations  are
determined  it is important  to consider  that
conditions  do  not remain  static.  On the
contrary, repeat monitoring and sampling  of
stations,  both  reference and  site  specific  will
need to be conducted   in order to document
change  over time and  further refine the  IBI.

Because  of subregional  differences,  further
demarcation  was made by examining  the role of
the basin or the watersheds within natural areas.
Natural areas  are similar to ecoregions  but are
demarcated  and established  along biotic
components.   Fish composition  and community
structure  is determined,  within a natural area  by
the availability of water of appropriate  quality
and  quantity to ensure  existence,   provide routes
of emigration,  sustain  growth, and  increase
fitness through  reproduction.  Likewise, species-
specific differences  exist  in community  structure
which  may  not  reveal  differences  in current
water quality but  may  be determined  by
historical  geomorphic   (Leopold et al. 1964)  or
zoogeographic   processes   (Hocutt and  Wiley
1986). Trends in Indiana water quality were
therefore  evaluated  using a basin approach
within an  ecoregion  framework.
Criteria for Selecting  Reference  Sites

Several  procedures  are available  for determining
reference  conditions.  Larsen et al. (1986) and
Whittier et al. (1987) chose  sites after  careful
examination  of aerial  photographs,   sub-basin
specific information review, and on-site
reconnaissance,   and  expert  consultation.  This
procedure  requires  that a limited  number of
high-quality sites be sampled  in order  to  predict
regional expectations.   The methods  chosen  for
site selection  were based  on the evaluation  of
Regional Water Quality Planning Maps (USGS
undated)  which identified known impact sources
and diffuse nonpoint  sources  which could
potentially influence  a site. A balanced
distribution of sites  within all parts of the
Maumee River drainage was maintained against
historic collections  sites (Jordan  1877; Gerking
1945; IDEM 1990).  Allsites  were rigorously
sampled in order  to get representative,  distance
specific, quantitative estimates  of species
richness and  biomass. Maximum  species
richness lines were then compiled  (see methods
below), followed by calculations  of the Index of
Biotic Integrity values  to reveal  which  stations
were the  "least impacted"  stations  for  the
Maumee  River drainage.

Reference  sites are defined as the stations
which  cumulatively  define  the 95th  percentile
line of the individual metrics. Evaluation of
habitat  and other physical  parameters   refined
the final list of reference  sites.  Sites  which had
habitat  or water quality deficiencies,  but still

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                                                                                  Huron-Erie Lake Plain
attained  high  index  ratings would  have been
removed from the final list. This action  was not
required, since poor habitat and water  quality
affected  various portions of the community
resulting in a  lowered index score.  These  sites
are not pristine or undisturbed  (few exist in
Indiana), but they do represent the best
conditions  given the background  activities (i.e.
anthropogenic  impacts;  channelization;  cultural
eutrophication).

Sampling was  conducted  in all size classes  of
river reaches  in the Maumee,  St. Joseph,  and
St. Mary's Rivers from the headwater (<20
mile2)  to the largest  mamstem  drainage  area
(ca.  1,000  mile2) in  Indiana.
2.0  STUDY AREA

Indiana has an  area  of 36,291 square  miles, and
drains the Ohio, the  upper Mississippi, and
Great  Lakes Regions  (Seaber  et al. 1984).
These three  regions  were further subdivided into
nine subregions  (Fig. 1), five of which drain 86%
of the State (USGS 1990). The State  of Indiana
lies within the limits of latitude 37° 46' 18" and
41° 45' 33" north, for an extreme length of 275.5
miles in a north-south  direction; and  between
longitude  84° 47' 05" and  88°  05' 50" west with
an extreme width in  an east-west direction  of
142.1  miles.

The  State  has a maximum topographic  relief of
about  900.9 ft, with elevations  ranging  from
about  300.3 ft above  mean sea  level at the
mouth of the Wabash River to slightly more than
1,201.2 ft in Randolph  County in the  east-central
part  of the state.

This report  considers  only the Maumee River
drainage.  The Maumee River drains  an area of
6,608 mile: (Hoggatt   1975). It crosses  two
ecoregions  and drains 5.5% of  Indiana. The St.
Joseph  River drains  the Eastern  Corn Belt  Plain
while the  Maumee  and St. Mary's River drain the
Huron-Erie Lake Plain ecoregions  (Omernik and
Gallant, 1988). The Maumee River is located in
northeastern  Indiana and  drains in a
northeastern  direction  into Ohio and  then  Lake
Ene. The Maumee  River is the  largest tributary
of Lake Erie. Tributaries of the  Maumee  which
drain  the  Eastern  Corn Belt Plain include the St.
Joseph  River, Cedar Creek, Fish Creek, and
Black River. The Huron-Erie Lake Plain in
Indiana includes  the upper Maumee  and  the St.
Mary's Rivers.
Physiographic  Provinces

Fenneman  (1946) divided the State  into two
physiographic  provinces  based  on the
maximum extent of glaciation.  The glaciated
portion of the State  contains  the Central
Lowland province,  which includes the majority
of the Maumee  River drainage, and  the
unglaciated  portion  is termed  the Interior Low
Plateaus province.

Schneider  (1966) further divided  Indiana into
three broad physiographic  areas  that  closely
reflect the  surface-water  characteristics  of the
State.  The Maumee  River drains  a portion  of the
Northern Lake and Moraine Region.

The Northern  Lake and  Moraine  Region  covers
the northern  one-fourth  of the State  and is of
variable relief. Its characteristic  deep  peat
deposits  and  small lakes are restricted to the
rugged, terminal moraines.  Numerous  broad
lacustrine  and outwash plains occur,  often
marked by wide marshes (or marshes  now
drained)  broken  by low sand ridges  or knolls.
The northern  section  of the  State was  covered
during the  most  recent Wisconsinan  glacial
event.

The last major glaciation  event dramatically
altered northern  Indiana during the Wisconsinan
period (14,000 to 22,000 years  ago). As glaciers
advanced  and  retreated,  the  land surface was
dramatically altered  as the  I and forms were
either scoured  by advancing  glacial  ice or the
scoured materials were deposited  by retreating
glaciers.  Two distinct glacial lobes are known
to have advanced  into Indiana, from the
northeast  out  of Lake Erie and  Saginaw  Bay
basins and from the north from the Lake
Michigan basin.

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Indiana  Ecoregions
  71
Great Lakes Basin
Mississippi  River
Ohio River
Regional  Boundary
Sub-Regional Boundary
Accounting  Unit  Boundary
                                                                     f\/\  Cataloging Unit Boundary
                                                                     1/Vj  County Boundary
                                                                           State  Boundary
Figure  1.   Map of  Indiana showing Major and  Minor drainage basins,  (from USGS data).
                                               4

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                                                                                   Huron-Erie Lake Plain
Ecoregions

Omernik  and Gallant (1988) characterized  the
attributes of ecoregions  of the midwestern
states.  Indiana  has six recognized  ecoregions:
Central Corn Belt Plain,  Huron-Erie Lake Plain,
Southern  Michigan-Northern Indiana Till Plain,
Eastern  Corn Belt Plain, Interior Plateau,  and
Interior River Lowland (Fig. 2). The Maumee
River basin drains portions  of the Eastern  Corn
Belt Plain and Huron-Erie Lake Plain ecoregions
(Omernik and Gallant (1988).

Eastern  Corn Belt Plain

Much  of the ecoregion  consists  of extensive
cropland  agriculture.  It is distinguished  from the
western  corn belt plains by the natural  forest
cover  and associated   soils. The gently  rolling
glacial till plain is broken  by moraines,   kames,
and outwash  plains. Elevations  range between
399.3  ft to greater than 1320 ft. The ecoregion  is
characterized  by low relief, typically  less  than 66
ft;  however,  some  morainal hills occur  in the
northern portion  near  Lake Erie. Stream  valleys
are long and sinuous and generally narrow and
shallow throughout the 31,800 miles2 of the
ecoregion.  Small streams have  narrow  valley
floors; larger streams  have  broad valley floors.
Elevation varies from about 399.3 ft, in  the
southern  portion  of the ecoregion,  to over 999.9
ft on a few of the hills in the north. Precipitation
occurs  mainly during the growing season  and
averages  from  35 to 40 inches  annually. The
ecoregion  has  few reservoirs or  natural  lakes.

Both perennial  and intermittent streams  are
common  in the ecoregion.  Constructed
drainage  ditches  and  channelized streams
further assist  in soil drainage in flat, poorly
drained areas.  Stream  density is approximately
one half mile per square  mile in  the most typical
portions  of the  ecoregion  (Fig. 2).

The ecoregion   is almost  entirely  farmland.  The
major  crops  produced  are com and soybeans.
A total of 75% of the landuse is cropland, while
the  remaining 25%  is permanent  pasture,  small
woodlots, or urban. Emphasis on livestock
 includes  the growing of feed  grains  and hay.
 Swine, beef and dairy cattle,  chickens,  and
 turkey  are  raised.

 Most of the soils were developed under the
 influence of deciduous  forest vegetation.  The
 soils are  loamy calcareous  glacial till,overlain
 by loess  deposits.  The soils are lighter in color
 and more acid  than  the  adjacent  Central  Corn
 Belt Plain.  Hapludolls and Ochraqualfs  are the
 dominant  soil groups on dry  and wet upland
 sites, respectively. Argiaquolls, Haplaquolls,  and
 Medisaprists have developed  in flats and
 depressions.  Hapludalfs and  Fragiudalfs are
 common  on well drained slopes of valleys.
 Shallow Hapludolls occur  on  some  valley sides
 where  erosion  has removed  the glacial  material
 and exposed  the underlying shale limestone.
 Udifluvents and  Fluvaquents have derived  from
 silty alluvium in narrow floodplains.

 The natural vegetation of the  area consists  of
 diverse hardwood  forests, predominantly
 American  beech  and  sugar maple. However, a
 significant amount of white oak, black oak,
 northern  red oak, yellow popular, hickory,  white
 ash, and  black walnut exists.  Many  of the trees
 are common in adjacent  ecoregions,  but most
 are comprised  of oak and hickory. Wetter sites
 include white oak, pin oak, northern  red oak,
 yellow popular,  ash,  and sweetgum  primarily,
 and  shingle oak, black oak, and hickory also
 occur.  Silver maple, cottonwood, sycamore,  pin
 oak, elm, and sweetgum  grow along rivers and
 stream  corridors.
Huron-Erie  Lake Plain

The Huron-Erie Lake Plain is discontinuous  and
is primarily distinguished  from surrounding
ecoregions  based  on  poor soil drainage.  Most
of the  area  was once  covered  by forested
wetlands.  Many wetlands are  still present, but
many have  been drained and  cleared  for
cropland.  Diverse cash crops  and livestock  are
principal land uses.  The ecoregion  consists  of
broad,  nearly level lake plain crossed  by beach
ridges  and  low moraines. The ecoregion  is
characterized  by areas  around 600  ft rising  to

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Indiana Ecoregions
                                                                        Central Corn Belt Plains
                                                                        Eastern Corn Belt Plains
                                                                        Northern  Indiana Till  Plains
                                                                        Huron/Erie  Lake  Plains
                                                                        Interior Plateau
                                                                        Interior River Lowland
     Figure 2. Map of Indiana showing the ecoregion designation ftomOmemik and Gallant (1988).
                                                   6

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                                                                                   Huron-Erie Lake Plain
800 ft in elevation  on some of the moraines.
Local relief is usually only a few feet. Streams
within the morainal hills and  valleys are often
intermittent becoming  perennial  when  they
reach the valley floors.

Large watersheds  in the southern  portion  of the
ecoregion  often drain  as  much  as 400-500
miles2 in the overall  11,000 miles2 of the
ecoregion.  The majority of streams  drain less
than 100 miles2. Precipitation is evenly
distributed throughout  the year and  averages
from 31 to 35 inches annually. The ecoregion
has few lakes and reservoirs  with those  present
usually being less  than a quarter mile2.

Numerous  drainage  ditches  have been
constructed   and many  streams  are extensively
channelized  allowing for quicker  agricultural
drainage in flat, poorly drained  areas.  More than
half of the streams  in the ecoregion  are
intermittent.  These intermittent streams  have a
density  of approximately  one-half miles per mile2
in the most typical portions of the ecoregion
(Fig. 2).

The ecoregion   has a broad range of land  uses
including  farmland which is used for cash  crops
and hay  for livestock.  Cora, winter wheat,
soybeans,  and  hay are principal crops. In
addition, sugar  beets,  field and  seed beans,  and
a variety of canning  crops are also grown. Fruit
and truck crops are grown on some coarse-
textured  soils.  Some farmland is maintained  in
pasture  and  small woodlots.  Livestock  includes
swine, dairy cattle, and chickens. Approximately
10% of the area is urbanized.

The extensive,   nearly level plains and  numerous
depressions   in  morainal areas are responsible
for the formation of poor and very poor drained
soils. The poorly drained  soils of the Huron-Erie
Lake  Plain support  swamp  forests.  Ochraqualfs
and Haplaquepts  formed in lacustrine  and
glacial drift. Udipsamments  and  Hapludalfs are
found on  beach  ridges and well drained sites.
The natural climax vegetation of the area
consists  of American elm, red maple, and  black
ash.  In parts of northern Ohio forest species
include silver maple, swamp  white oak,
sycamore,  pin oak, blackgum,  and  eastern
cottonwood.
Natural Areas

A natural  region is a major, generalized unit of
the landscape  where  a distinctive assemblage  of
natural  features is present  (Homoya  el al. 1985).
It is similar to the ecoregion  concept  integrating
several  natural  features,  including climate, soils,
glacial history, topography,  exposed  bedrock,
presettlement  vegetation,  and physiography. It
differs from the ecoregion  concept in the
utilization of biodiversity of the fauna  and flora
to delineate areas of relative homogeneity.

The Maumee  River drainage  incorporates  the
Black Swamp and Central Till Plain Natural
Regions (Fig. 3).  The Central Till Plain Natural
Region  is compossed   of three sections. The St.
Joseph  River drains  the Bluffton Till Plain
Section.  Only the Bluffton Till Plain Section  is
appropriate  for this discussion.

The Central Till Plain is the largest  natural
region in Indiana, formerly considered  a part of
the forested  Wisconsinan  till in the central
portion  of the state. The Region  is
topographically  homogeneous   although glacial
moraines  are common.  The region is a major
divide between  the biotic communities   with a
strong northern affinity and those with strong
southern affinity. The Entrenched  Valley is a
concentrated  continuum of northern,  southern,
eastern  and  western  affinities. The Bluffton Till
Plain  subsection  is the  predominant  subsection
of the St. Joseph  River drainage.  The Bluffton
Till Plain is characterized  by  clay rich soils on  a
relatively level till plain. This section in
conjunction with the Black Swamp,  Northern
Lakes and Northwestern Morainal Natural
Regions was one of the last areas  covered  by
the Ontario-Erie Lobe of the Wisconsian ice
sheet.  A distinct series  of moraines  occurs  with
the southern border  marked  by the Union City
Moraine.

The soils are predominantly clay till which
provides poor drainage.  The acid to neutral  silty

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 Indiana Ecoregion
                                                                                        HOMOYA'S NATURAL REGIONS
                                                                                                  OF INDIANA
                                                                                                      NORTHERN LAKES


                                                                                                      GRAND PRAIRIE—Kankakee Marsh


                                                                                                      GRAND PRAIRIE—Kankakee Sand


                                                                                                      GRAND PBAIRIE — Grand Paririe

                                                                                                      NORTHWESTERN MORAINAL— CNcagoLake
                                                                                                                      Basin

                                                                                                      NORTHERWESTERN MORAINE—Valparaiso
                                                                                                                       MoSne

                                                                                                      NORTHWESTERN MORAINAL — Lake Michigan
                                                                                                                       Border

                                                                                                      CENTRAL TILL PLAIN — Tipton Till Plain


                                                                                                      CENTRAL TILL PLAIN — Entrenched Valley


                                                                                                      CENTRAL TILL PLAIN -Bluffton Till
                                                                                                                   Plain

                                                                                                      BLACK SWAMP

                                                                                                     BLUEGRASS — Muscatatuck Flats and
                                                                                                              Canyon

                                                                                                     BLUEGRASS — Scottsburg


                                                                                                     BLUEGRASS — Switzerland Hills


                                                                                                     SHAWNEE Hm—Crawford Upland


                                                                                             §^|  SHAWNEE HILL — Escarpment


                                                                                             |H  SOUTHWESTERN LOWLANDS — Glaciated


                                                                                             HI  SOUTHWESTERN LOWLANDS — Plainville


                                                                                             ^H  SOUTHWESTERN LOWLANDS — Drifiless


                                                                                             I    I  HIGHLAND RIM — Mitchell Karst Plain


                                                                                             iJJaSi  HIGHLAND RIM — Brown County Hills


                                                                                             tv;.^  HIGHLAND RIM — Knobstone Escarpment


                                                                                             ^^1  SOUTHERN BOTTOM LANDS
Figure 3:  Map of Indiana indicating the natural areas designation of Homoya et al.
(1985)

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                                                                                Huron-Erie Lake Plain
clay loams of the Blount-Pewano-Morley
Associations  characterize  the region.  The
forested community associated  with these
poorly drained soils were  ubiquitous but are
now confined to the scattered  woodlots within
the region. A species  common  to the woodlots
is swamp cottonwood.  Minor areas  of bog,
prairie, fen, marsh, and  lake communities  are
comprised  of cottongrass,  northern St. John's
wort, pitcher plant, and  sedges.  Two southern
swamp species  are geographically   restricted,
including swamp  St. John's  wort and  log sedge.
The Black Swamp  Natural Region  is the western
lobe of a large lacustrine plain occupying
ancient Lake Maumee.  Lake Maumee,  a
predecessor  to modern  Lake Erie, was created
when the meltwater of the Ontario-Erie Lobe of
the Wisconsinan  ice sheet was  dammed  by the
Fort Wayne Moraine. The Maumee and St.
Mary's River drainages  occur within this Natural
Region. Much  of the area is nearly level and
poorly drained.  Streams   are low-gradient,  silty
and shallowly entrenched. This area  is the same
as  Mallot's Maumee Lacustrine  Section of the
Northern  Moraine and Lake Region.  Early
settlers named  the Black Swamp because   the
natural communties  included swamp forests
dominated by American elm, black ash, and  red
maple. Additional species include bur oak,
swamp white oak, white ash, shellbark  hickory,
pawpaw,  and  spicebush.  These species  have
been  virtually eliminated  from this region  in
present day Indiana.  The soils are  typically
deep,  acidic to neutral clay and silt loams of the
Hoytville-Nappanee  Association.
Drainage Features

Three major  drainage  units occur in the Huron-
Erie Lake Plain of Indiana: the Maumee  River,
St. Joseph  River, and  the St.  Mary's River
drainages.
Maumee River

The Maumee River begins  at the junction of the
 St. Joseph  and  St. Mary's Rivers and  consists
 otherwise of only minor tributaries  in Indiana.
 The Maumee  River basin contains  aquifers
 which provide significant ground-water
 contribution to streamflow.  During dry years
 ground water contributes 27% of the stream
 flow, while direct surface  runoff accounts   for 73-
 92% of the stream flow (Indiana  Department  of
 Natural Resources  1980). The Maumee River
 drains 5.5% of the State.  The Maumee River
 flows northeast  as a major  tributary of Lake
 Erie. Direct tributaries  of the Maumee  within
 Indiana include  Gar Creek,  Black Creek,
 Flatrock Creek,  and  Mars Ditch.  The minor
 tributaries fluctuate with seasonal  flows. The
 Maumee River varies dramatically with
 contributions   from the St. Joseph and  St. Mary's
 Rivers and baseflow from groundwater. Average
 discharge for the Maumee River during 1991,
 upstream  of the Norfolk and Western  railroad
 bridge, near New Haven, was  1,828 cfs. Flow
 ranged  between  45.0 cfs during  the 7 day, 10
 year low flow to 26,600 cfs  during  maximum
 discharge during the period of record  (Arvin,
 1989).
St. Joseph  River

The St. Joseph  River drainage  is the major
northern segment  of the Maumee  River
(comprising  1,060 miles2) which joins with the
St. Mary's River at Fort Wayne. The St. Joseph
River has been  impounded  at numerous
localities above Fort Wayne, and receives  a
substantial  amount  of its streamflow  from
surface  water. Tributary segments  of the St.
Joseph  River in Indiana includes  Cedar Creek,
Fish Creek,  Bear  Creek, and Yohe Ditch. The St.
Joseph  originates  in a different ecoregion and
natural area than the rest of the Maumee  River
drainage. The average discharge  of the St.
Joseph  River near Fort Wayne (Allen County
downstream  from Ely Run) during 1991 was
1,019 cfs. Discharge ranged  from 1.3 cfs during
the 7 day, 10 year low flow to 13,200 cfs during
maximum flow periods (Arvin, 1989).

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St. Mary's River

The St. Mary's River drainage is the major
southeastern  segment  of the Maumee River
(draining 762 miles2) which connects  with the
St. Joseph  River near Fort Wayne, Allen County.
The St. Mary's River has fewer impoundments,
and receives  a substantial  amount  of its
stream/low from surface  water. The River
emanates  southeast  of Fort Wayne and is
formed  in the state of Ohio. Tributary segments
of the St.  Mary's River include Blue River, Little
Blue River, Yellow Creek, and Holthouse ditch.
The St. Mary's River lies solely within the Huron-
Erie Lake Plain and Black Swamp natural  area.
The average discharge of the St. Mary's River
near  Ft. Wayne (Allen County downstream  from
Anthony Boulevard extension  bridge) was  942
cfs during 1991. Discharge  records ranged from
0.2S cfs during the 7 day,  10 year low flow to
13,600 cfs during  maximum  flow periods  (Arvin,
1989).
Historical Maumee River Data

The Maumee River is the largest tributary of
Lake Erie and historically was stated  to be  one
of Indiana's highest quality resources.  The  first
use  of the Maumee River was as a commercial
connection   for fur traders  between  the Great
Lakes and  the Mississippi River. Since  then the
Maumee River has been intensively examined
including its importance  as a trade route  (Fatout
1985); hydrology (Pettijohn and Davis 1973;
Hoggatt  1981); surficial geology  and
physiography  (Leverett 1902; Pettijohn and
Davis 1973); Paleozoic  and Quartemary geology
(Droste and Orr 1974; Doheny et al. 1975;
Sunderman  1987); geomorphic  contribution of
the Maumee River during  the draining of Lake
Maumee (Bleuer 1989); groundwater  flow
(Pettijohn and Davis 1973); nutrient and
sediment  transport (Richards and Baker 1991);
sediment  contamination  (Sobiech and  Sparks
1992); agricultural  nonpoint  sources  (Logan
1981; Antosh 1991; Baker 1991; Krieger 1991;
Yoder 1991); pesticide  toxicity (Biever and
Giddings  1991; Dickson and Tiemey 1991); and
bioaccumulation  (Sobiech and  Sparks  1992).
The aquatic  communities  of the Maumee River
have been correlated  with water quality (Logan
1981).  Various components  of the aquatic
community of Lake Erie tributaries  have been
studied  including the  microbial community  (Pratt
1991),  algal  community (Lowe 1991),
macroinvertebrates  (Krieger and Deshon 1991),
while the mussel  fauna was studied  by Clark
and Wilson (1912) and  Waiters  (1991). The fish
community has also been  well studied  including
distribution (Kirtland 1844, 1847; Meek 1889;
Kirsch  1895;  Gerking  1945; Van Meter and
Trautman  1970;  Pearson and Shipman  1978;
Trautman  1981);  population dynamics of
stocked fish  (Pearson  and Shipman   1978;
Pearson  1984); endangered   species   status
(Simon  1993); and  fisheries potential (Klippart
1877; Braun  1993).

The Maumee River possesses  a highly diverse
fish community.  Previous studies have
documented   a total of 102 species  of fish in the
Maumee River basin (Simon et  al. in press). The
earliest  records of Jordan  (1877) suggest the
river was abundant  with both food  and non-
game species. The Maumee River received a
large amount of collection effort by early
ichthyologists.

Kirsch (1895) collected at five locations  in the
basin.  Kirtland (1844,  1847) documented
species  assemblages   in Ohio portions  of the
drainage.  Gerking (1945) collected  at 10
localities in the St.  Marys', St. Joseph,  and
Maumee Rivers.  Clark and Allison (1966)
documented   species  trends  in the Maumee  and
Auglaize Rivers of Ohio.

3.0 MATERIALS AND  METHODS

Sampling

  Site Specific

In order to answer  basin-specific questions   and
to calibrate  an IBI for evaluating  ecosystem
health,  a sufficient number of samples  were
required from each of the various drainages.  A
total of 77 locations (Fig. 4) were surveyed
during  June  through  August 1991 in order  to
                                                  10

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                                                                                   Hi
                                      I Mice Plain
 compile the data needed  to evaluate  the
 maximum species  richness lines  for calibration
 of the Index of Biotic Integrity. Site and
 collection records  are maintained  within the
 State of Indiana files. Since the primary  purpose
 of this study was to evaluate  the  water quality of
 Indiana using  biological methodology,  no further
 evaluation of site specific data  (e.g. site specific
 taxonomic  species  lists) will be included other
 than  an overall taxa list for each sub-drainage.

 To ensure repeat sampling  at the exact  same
 site, all locations are based on latitude and
 longitude. Narrative descriptions for mileage are
 from the  center  point rather than  the  edge of
 the nearest town since  the  boundaries of many
 Indiana towns will change  over the next century.
 All sites were evaluated based on drainage  area,
 since this provides  a reliable  quantification
 (Hughes  e» al. 1986) of stream  size.  As drainage
 area  increases   fewer locations are available  for
 comparative  analysis.
  Habitat

The diversity of habitats  sampled  has a major
effect on  data collection.  A representative
sample  always  requires that the entire range of
riffle, run, pool, and extra-channel   habitat be
sampled,  especially when  large rivers are
surveyed. Atypical samples  result when
unrepresentative  habitats  are sampled  adjacent
to the sampling site. Species  richness  near
bridges or near the mouths of tributaries
entering large rivers, lakes,  or reservoirs  are
more likely to be characteristic  of large-order
habitats than the one under  consideration
(Fausch  «f  al. 1984).

A general site description  of each  established
sampling  location was conducted  using the field
observation   procedure  of Ohio  EPA (1989) and
Rankin  (1989). The Quality Habitat Evaluation
Index (QHEI) takes  into account  important
attributes  of the habitat which increases
heterogeneity.  Scoring incorporates  information
on substrate  composition,  instream  cover,
channel morphology,  riparian zone and bank
erosion, and pool and  riffle quality.
 Physical/chemical  parameters  were recorded
 for each sample site to assist in assessing  the
 biological  data  further: dissolved oxygen, pH,
 temperature,  and specific conductivity.
 Equipment  utilized for physical  water quality
 analysis was  a  Hydrolab SVR2-SU meter
 following the  specifications  of the manufacturer.
  Community  Analysis

Sample Considerations

Only one electrofishing gear type need  be used
at each location to collect a representative
sample (Jung  and Libosvarsky  1965; Ohio EPA
1989). A T&J pulsed-DC generator capable  of
300 volt output was mounted  in a Coleman
Sport-canoe,  floated in a Sport-Yak,  or attached
to a long-line. We collected by wading  in
shallow riffles and runs,  and floated  through
pools  and unwadeable   habitat.

All fish encountered  were collected  at each site.
Adult and juvenile specimens  from each stream
reach  were identified to  species   utilizing the
taxonomic keys  of Gerking (19SS), Trautman
(1981), and Becker (1983). Cyprinid  taxonomy
follows Mayden  (1989), changes  in species
nomenclature   is listed  in Appendix D for
comparability   with previous investigations.
Generally, young-of-the-year  fish less than 20
mm in length  were  not included  in Index of
Biotic Integrity or composite  totals analysis.
Early life stages  exhibit high initial mortality
(Simon 1989) and  are  difficult to collect with
gear designed  for larger fish (Angermeier  and
Karr 1986). Collection  of fish from this category
will be retained for possible future use in State
water monitoring programs  (e.g.
ichthyopiankton  index  (I2)).

The length of stream reach  sampled  is  an
important consideration.  Karr ef  al. (1986)
recommended   in larger streams   to select
several contiguous  riffle-pool sequences   rather
than relying on a standard  length. When
electrofishing  equipment  was  employed  in larger
rivers (i.e. > 1,000 mi2),  samples  were taken  in
                                                   11

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Indiana Ecorerion
units of 0.5 to 1.0 km (Gammon et al. 1981).
The length of the sample  reach  was long
enougd to include  all major habitat types.
Distances of  11 to IS stream widths were
generally  adequate   to sample two cycles of
habitat  (Leopold et al. 1964). Ohio EPA (1989)
suggested  that after 150-200 m of stream  length
no  significant increases  in the IBIare observed,
however species richness  may still increase  until
250-300 m. The additional  increase in effort is
not justified by the assessment   capability  of the
index so the minimum distance  of 15 times  the
mean stream  width was adopted.  Additional site
information (e.g. photographs;  latitude  and
longitude)  were recorded  on the data  sheet.

Selecting  the appropriate  time of year for
sampling  is critical. Kan et al. (1986) found that
periods of low-to moderate  stream flow are
preferred and  the relatively variable flow
conditions of early spring and late
autumn/winter  should  be avoided.  Species
richness tends  to be higher later in summer  due
to the presence  of young-of-the-year  of rare
species,  but this can be  avoided if data analysis
does  not incorporate  young-of-the-year  species.
Samples  of limited area may be less  variable in
early summer  than  comparable  samples taken
later in the year. Each site was  sampled for a
single pass on both shorelines for nonwadeable
locations.  .
 Sample  Site Selection

 Fish sample sites were selected  based upon
 several factors:

 1). Choosing  stream reaches  not  affected
    by point source  dischargers;

 2). Stream use  issues  (i.e. municipal
    treatment  works, non-point source,
    nutrient reduction);

 3). Location  of physical stream  features
    (e.g. dams,  changes in geology, changes
    in stream  order, presence  of stream
    confluence,  etc.);
4).  Location of non-point sources  of
    pollution (e.g. urban  areas  or obvious
    farm runoff);

5).  Variations in habitat suitability for fish;

6).  Atypical habitat not representative  of
    River reach or basin.

Whenever possible, sites were located  upstream
from pollution sources  and adjacent  tributaries
(Gammon 1973). Stations were selected  to
include natural areas,  parks (Federal, State,
County, and Local), exceptional  designated
streams,  and from historical sampling locations
whenever available.

When non-impacted areas  were  not  present,
"least impacted" areas  were selected  based on
the above criteria.  Sites were  chosen  which
indicated  recovery  from channelization or
potential non-point source  areas,  and which had
a suitable riparian  buffer on the shoreline.  When
a series of point source dischargers  were
located on a river, every effort was made to
sample  upstream  of the discharger or to search
for areas  of recovery between  dischargers
(Krumholz 1946).

When  impoundments  or other physical habitat
alterations had been imposed  on a .l.sr,
sampling  was conducted  in the tailwaters of a
dam (area immediately  downstream).  Tailwaters
possess  the greatest resemblance  of the lotic
habitat. The serial  discontinuity concepts   of
Stanford et al. (1988) predicts  that the thermal
character  of a stream  below a dam will be
"reset" toward  that typical of the stream  reaches
above  the dam.  In areas  where  sampling  could
not be accomplished   downstream  of the
physical structure  due  to lack  of access,   stream
tributary segments  were located  upstream of the
dam away from the immediate influence  of the
pooled  portion. Likewise, bridges were always
sampled on the upstream side, away from the
immediate vicinity of the structure  and latent
bridge construction effects.

Fish from each  location  were identified to
species and enumerated.  Smaller and more
                                                    12

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Figure 4       Maumee  River drainage indicating the  location  of sampled  locations
               during  1991.

                                     13

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difficult to identify taxa were preserved  for later
examination  and identification in the laboratory.
All fish were examined  for the presence  of gross
external  anomalies.  Incidence of these
anomalies was defined as the presence  of
externally visible morphological  anomalies (i.e.
deformities,  erosion,  lesions/ulcers).   Specific
anomalies include: anchor  worms;  leeches;
pugheadedness;   fin rot; Aeromonas  (causes
ulcers, lesions, and skin growth, and formation
of pus-producing  surface  lesions  accompanied
by scale erosion);  dropsy  (puffy body);  swollen
eyes; fungus; ich; curved  spine; and swollen-
bleeding mandible or opercle.  Incidence  is
expressed  as percent of anomalous  fish among
all fish collected.  Incidence  of occurrence  was
computed  for each  species  at each  station.

Hybrid species  encountered   in the field (e.g.
hybrid ceotrarchids,  cyprinids) were recorded
on the data sheet,  and when possible,  potential
parental  combinations  recorded.
Index of Biotic Integrity

The ambient environmental  condition  was
evaluated  using  the Index of Biotic Integrity
(Karr 1981; Karr et al. 1986). This index relies on
multiple parameters (termed "metrics") based  on
community concepts,   to evaluate a complex
biotic system.  It incorporates  professional
judgement  in a systematic  and  sound manner,
but  sets quantitative  criteria that enables
determination  of a continuum between very
poor and  excellent based on species  richness
and composition,  trophic and reproductive
constituents,  and  fish abundance  and condition.
The twelve original Index of Biotic Integrity
metrics  reflect insights from several perspectives
and cumulatively are  responsive to changes  of
relatively small magnitude,  as well as broad
ranges  of environmental degradation.

Since  the metrics  are  differentially sensitive  to
various  perturbations   (e.g.  siltation or toxic
chemicals),  as well as various degrees or levels
of change within the  range  of integrity,
conditions  at a site can  be  determined with
considerable  accuracy. The interpretation of the
index scoring is provided  in six narrative
categories  which have been tested in the
midwestem  United States  (Karr 1981; Table 1).

Several  of the metrics are drainage  size
dependent  and require calibration to determine
numerical scores  (Tables  2-3). The ecoregion
approach  developed  by USEPA-Corvallis,
Oregon,  was utilized to compare   "least
impacted" zones  within the  region (Omernik
1987). Ohio EPA (1987),  modified several of the
original 12 metrics  in order  to make them more
sensitive to environmental effects  based  on their
experiences   in Ohio  and  to account  for stream
and  river size, fauna! differences,  and  sampling
gear selectivity. The  current study utilizes the
experiences   of the Ohio EPA and Karr et al.
(1986) in developing  an IBI for Indiana streams
     rivers.
Metrics

In general, the metrics utilized for the current
study are those developed  by the State of Ohio
(Ohio EPA 1989) for analysis  of surface water
designated use-attainment.  This includes
modification  of several of the original Index of
Biotic Integrity metrics as proposed   by Karr
(1981).

Although the  methodology  and application  of
the ecoregional  expectations   are  similar in
approach  to Ohio and much of the  information
below is taken directly from  the Ohio document
(Ohio EPA 1989), a significant difference exists
between  the Indiana and Ohio reference
conditions. This difference  exists  in how the
metric expectations  are developed.  In Ohio, the
ecoregional reference  stations were  combined
into a single data set for the entire State,  and
later modifications  were developed  for the
Huron-Erie Lake Plain. In Indiana, "least
impacted"  conditions  are being developed  on a
regional  basis, with a priori recognition  of basin
differences within ecoregion,   based   on the
natural division classification  of Homoya et al.
(1985). Further evaluation at the completion  of
the
                                                    14

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                                                                                   Huron-Erie Lake Plain
Table 1. Attributes of Index of Biotic Integrity (IBI) classification,  total IBI scores,
        and integrity classes  from Karrer aL (198$).
Total IBI
score
 Integrity
   Class
Attributes
58-60
48-52
40-44
28-34
12-22
Excellent       Comparable  to the best situation without human  disturbance;  all
                regionally  expected species  for the habitat and stream size,
                including the most intolerant forms, are present  with a full array of
                age  (size)  classes;  balance  trophic  structure.

Good           Species  richness  somewhat  below expectations,  especially  doe to
                the loss of the most  intolerant  forms;  some  species  are present
                with less than  optimal  abundances   or size distributions; trophic
                structure shows  some  signs of stress.

Fair            Signs of additional deterioration include loss of intolerant  forms,
                fewer species,  highly skewed  trophic structure  (e.g. increasing
                frequency  of omnivores and other  tolerant species);  older age
                classes  of top predators  may be rare.

Poor            Dominated  by omnivores,  tolerant  forms,  and habitat  generalists;
                few top carnivores; growth rates and condition factors commonly
                depressed;   hybrids and diseased  fish often  present.

Very Poor      Few fish present,  mostly introduced or tolerant forms; hybrids
                common;   disease,  parasites, fin damage,  and other anomalies
                regular.

No Fish        Repeated   sampling finds no fish.
of the study  will determine if differential metric
treatment is warranted for basin  specific or
larger scale  criteria development.

The Index of Biotic Integrity  is sensitive to
differences  in collection effort and  gear type.  In
order to account  for these inherent biases,
separate  expectations   are developed  for each of
the two stream  classification  types utilized in  the
current study. Headwater  stream sites (<20
miles2)  were primarily sampled  for 50-100 m
using wading techniques.  These  sites were
sampled  using a  long-line configuration usually
                                        off bridges, while larger wadable rivers (> 20-
                                        1000 miles2) were  sampled  using the sport-yak
                                        configuration. This technique  requires  a
                                        sampling  distance  of 100-300 m and wading in
                                        all available habitats.

                                        Below is an explanation of each  of the  twelve
                                        metrics utilized for the calibration of the Indiana
                                        Index of Biotic Integrity for the Huron-Erie Lake
                                        Plain.  Due to inherent differences  at
                                        approximately  20 miles2 drainage area,  different
                                        metrics were  necessary  to evaluate both
                                        headwater  (< 20 miles2 drainage  area)  and
                                                   15

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                                                                                  Huron-Erie Lake Plain
Table 3. Index of Biotk Integrity metrics used to evaluate wadable river
(> 20-1,000 miles2 drainage area) sites in the Maumee River drainage.
Metric
Category
Species
Composition





Metric
Total Number of Species
Number of Darter Species
Number of Sunfish Species
Number of Sucker Species
Number of Sensitive Species
% Tolerant Species
Scoring Classification
5 3 1
>23 16-23
>4 2-4
>4 2-4
>4 2-4
>7 4-7
<15% 15-30%
< 16 (Fig. 5)
<2 (Fig. 6)
< 2 (Fig. 7)
< 2 (Fig. 9)
< 4 (Fig. 10)
> 30% (Fig. 11)
Trophic        % Onmivores1
Composition    < 1,000 square  miles

                % Insectivores'
                < 1,000 square  miles
Fish
Condition
% Carnivores'


Catch per Unit Effort

% Simple Lithophils

% DELT anomalies'
            15-30%    > 30% (Fig. 12)


>65%      40-65%    < 40% (Fig. 13)

Varies with drainage  area  (Fig. 15)


Varies with drainage  area  (Fig. 16)

Varies with drainage  area  (Fig. 17)

<0.1%     0.1-1.3%  >1.3%(Fig. 18)
'  Special  scoring  procedures  are required when less than  100 individual fish are collected.
observed  within the three basin segments,   the
segments  were pooled to reflect an ecoregional
consensus.  Likewise, if no relationship  with
increasing  drainage area was observed, the
maximum species  richness  lines either leveled
off at the  point where no additional  increases
were exhibited or horizontal  plots were
delineated  indicating no  increase with drainage
area.

Differentiation between headwater and  wadable
stream  and  river sites are indicated  on the
                                        graphs  by a vertical dashed  line on the
                                        appropriate metrics. This relationship was
                                        determined by searching  for bimodal patterns  in
                                        the basin specific  data  set plots of species
                                        richness.  A sixth order  polynomial defined
                                        where a significant bimodal  effect was evident
                                        for each of the drainage basins (Simon  1991).
                                        The tails of distribution of the data  are not
                                        significant. However the point where the data
                                        differentiates  into two distinct peaks suggest
                                        that the transition  between headwater  and
                                        wadable streams  is at 20 miles2 and between
                                        wadable and  large rivers at  1,000 miles2.
                                                   17

-------
Indiana  Ecorarion
Metric 1. Total Number  of Fish Species   (Headwater  and Wadable  Sites)
Impetus

This metric is utilized for all of the stream
classification  types used for calibrating the
Indiana Index of Biotic Integrity. Unlike the Ohio
metric,  exotic species  are  included in the  total
number of taxa. The premise  behind  this metric
is based on the observation that the  number  of
fish species  increases  directly with
environmental complexity  and quality of the
aquatic  resource  (Karr 1981;  Karr et  al. 1986).
Although  the number of exotic or introduced
species  may be indicative of  a loss of integrity
(Karr et al. 1986; Ohio  EPA 1989), the
differences between lower levels of biotic
integrity resolution may be due to colonization
of habitats by pioneer or tolerant taxa which
tend to incorporate  exotic species.

This single metric is considered  to be  one of the
most powerful metrics  in resolving water
resource  issues since a direct correlation  exists
between high quality resources  and  the
numbers  of species  for warmwater assemblages
(Ohio EPA 1987; Davis and Lubin 1989; Plafkin
et al. 1989; Simon,  1991). As total number of
        increases,  species become  more
specialized  and have narrower niche breadths,
numerous  higher level interactions  occur and
presumably  enable  greater efficiency in
resource  utilization. The delimitation between
headwater and wadable  Indiana streams  in the
Central Corn Belt Plain ecoregion  was  made
primarily on the data  from this metric.
Headwater  and wadable streams are
differentiated at 20  miles2 drainage  area.
Headwater and Wading Sites

The number of species  is strongly  correlated
with drainage  area  at headwater  and wadable
stream and river sites up to ca. 1,000 miles2.
Determining the Index of Biotic Integrity scoring
criteria for this metric did not require the
recognition of basins. Comparison  of maximum
species  richness lines for the appropriate  basin
and drainage  area  did not reveal any  significant
differences between ecoregion or subbasin (Fig.
5; headwater  and wading sites).
                                                  18

-------
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Metric 2. Number  Darter, Madtom, &Sculpin Species   (Headwater  _<_20  miles2)
             Number  of Darter Species   (Wadable  Rivers  > 20-1,000  miles2)
Impetus

Karr et  al. (1986) indicated  that the presence  of
members  of the tribe Etheostomatini are
indicative of a quality resource.  Darters require
high dissolved  oxygen  concentrations,  are
intolerant of toxicants  and siltation, and thrive
over clean substrates.

Life history  information for all of the 28 Indiana
species  indicates darters are insectivorous,
habitat  specialists,  and sensitive to physical and
chemical  environmental  disturbances  (Page
1983; Kuehne and  Barbour  1983). Darters are
excellent  indicators  of a quality resource,  and
are generally found in riffle habitats.

Headwater  Streams

The darters  include the  genera:  Amqnocrypta.
Crvstallaria. Etheostoma.  and PeTqna.   Of the
28 species recorded  from Indiana,  six are
commonly found throughout the State and  are
not restricted  to a particular stream  size
(Gerking  1945). Thirteen of  these 28 species  are
confined  to the Ohio River basin; none of the
species  are restricted  to the Mississippi River
basin; and a single  species   occurs  only in the
Great Lakes  drainage  (Table 4).

For sites  having drainage areas  less than 20
miles2, this metric also  includes  members  of the
family Cottidae (sculpins) and Ictaluridae
(madtoms;  genus  Noturus).  The sculpins  and
madtoms  are benthic insectivores  and
functionally occupy  the same type of niche as
darters. Their inclusion  enables  a greater  degree
of sensitivity in evaluating  streams  that "naturally
have significantly fewer darter  species. By
adding madtoms  and sculpins  this metric
asymptotes  with increased drainage  area  (Fig.
6). The number  of benthic insectivores was
found to remain static with increasing drainage
area  for each of the three  basins. In the
Maumee  River drainage, few darters  occurred
so this metric  was estimated  based on the total
number of species  which could be expected
rather man observed  during  the  current study.
No differences  in ecoregion  expectations   were
observed  between sites of the Huron-Erie Lake
Plain and  Eastern  Corn Belt  Plain.

Wadable  River Sites

Due  to a reduction  of quality sites  within greater
drainage  area  categories  for the Maumee  River
drainage  the expected number of sculpin  and
madtom  species declines.  Thus, only the
number of darter species  are included in
cumulative scoring  for drainage  areas greater
than  20 miles2 due  to inconsistency  in sampling
and  the patchy distribution of sculpins and
madtoms  in small rivers (> 20-1000 mi2). This
conforms  with the original IBI and  is consistent
with the Ohio  metrics established  for the Huron-
Erie  Lake Plain (Fig. 6).
                                                  20

-------
                                                                              Huron-Erie Lake Plain
Table 4. The distributional characteristics of Indiana darter (Etheostomatini),
madtom (Noturus). and sculpin (Cottus) species.
Distribution in Indiana Drainaees

Species
Arflflnncrvpta pellucida
A. clara
Crvstallaria asprella
Etheostoma asprigene
E. HeT"iinides
E. caeruleum
E- qnw"11"
E. chJoj32S°IQ&
E. exile
E. flabellare
E. gracile
E. histrio
£. iHflnirtfitofH
E. microperca'
E- nigrum
E. spectabile
£. squamiceps
E. tippecanoe
E. variatum
E. zonale
Percina caprodes
£. copelandi
£. evides
£• IWTI'Hf
P. phoxoceohala
P. sciera
P. sflflijmrdi
P. vigil
Noturus eleutherus
N. flavus
N. gvrinus
N. insiynis
N. miurus
N. nocturnus
N. stigmosus
Cottus bairdi
C. carolinae
C. cognatus


Statewide
X

X
X

X

X

X



X
X





X


X



X

X
X



X
X



Ohio Great Mississippi
River Lakes River

X X


X

X

X

X
X
X
X X

X
X
X
X
X X

X
X

X X
X
X X

X


X
X X
X


X
X
1
Restricted to northern portions of these drainages.
                                                 21

-------
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-------
                                                                               Huron-Erie Lake Plain
Metric  3. Number of Sunfisb  Species  (Headwater  and  Wadable Sites)
Impetus

This metric foUowed Karr (1981) and Karr et al.
(1986) by including the number  of sunfish
species  (family Centrarchidae) and excluding
the black  basses  (Micropterus spp). Unlike the
Ohio metric, the redear sunfish Lepomis
microlophiig is included  because  it is native to
Indiana (Table 6). Hybrid sunfish are not
included  in this metric following Ohio EPA
(1989).

This metric is an  important measure  of pool
habitat quality. It includes  all members  of the
sunfish genera  AfffrfTlife8 (rock bass),
Centnucfaus (round sunfish), Lepomis  (sunfish),
and  Pomoxis (crappies), as well as, the
ecological  equivalent  Elassomatidae  (Elassoma
zonatuml.  Sunfish normally occupy slower
moving water  which may act as "sinks" for the
accumulation  of toxins and  siltation. This metric
measures  degradation  of rock substrates  (i.e.
gravel  and boulder) and instream  cover (Pflieger
1975; Trautman  1981), and the associated
aquatic macroinvertebrate  community  which are
an important  food resource  for sunfish  (Forbes
and  Richardson  1920; Becker 1983). Sunfish are
important components   of the aquatic
community since they are wide  ranging, and
distributed in most  streams  and rivers of
Indiana. They  are also very susceptible  to
sampling  using electrofishing  gear. Karr et al.
(1986) found sunfish to occupy  the intermediate
to upper  ends of sensitivity of the index of biotic
integrity  (161).

Headwater and Wadable Streams and Rivers

Sunfish colonization is limited by the amount  of
pool habitat in many  river reaches.   This metric
did not show any difference  in scoring  based  on
ecoregion  or sub-basin.  The number of sunfish
species is not affected  by increasing drainage
area (Fig. 7).
Table 5. List of Indiana sunfish
           species  for evaluating
           quality pool  habitat.
Common  Name
Scientific Name
Rock  bass

Flier


Green sunfish

Pumpkinseed

Warmouth

Orangespotted
sunfish

Bluegill

Longear sunfish

Redear sunfish

Spotted  sunfish

Bantam  sunfish

White crappie

Black crappie

Banded  pygmy sunfish
Ambloplites

Centrarchus
Lepomis cvanellus

L. gibbosus

L. gulosus


L. faumilis

L. macrochirus

L. megalotis

L.microlophus

L. punctatus

L. svmmetricus

Pomoxis annularis

P. nigromaculatus

Elassoma  zonatum
                                                 23

-------
to
                                      Wading/Headwater   Sites

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Drainage Drainage Drainage



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                  Figure 7.  Maximum species richness lines for determining trends in number of sunfish species
                          with increasing drainage area for the Maumee River drainage.

-------
Metric 4. Number  of Minnow  Species  (Headwater  Sites)
            Number  of Sucker Species   (Wadable Sites)
Impetus

The original Index of Biotic Integrity metrics
included the number  of sucker species (Karr
1981; Karr el al. 1986). Suckers  represent a
major component  of the Indiana  fish fauna since
their total biomass usually ranks  them among
the highest  biomass  contributors  in the
community. Most sucker  species  are intolerant
to habitat and water quality degradation  (Phillips
and Underbill 1971; Karr et al. 1986; Trautman
1981; Becker  1983) and this results in metric
sensitivity at ths higher end of environmental
quality. Suckers,  due to their long  life span (10-
20 years),  provide a long-term assessment   of
past environmental conditions. Of the 19
species  historically found in Indiana,  Lapochila
lacera  is considered  extinct, seven species  are
widely distributed  throughout  the State (Table
6). Extant sucker genera  include:  Cvcleptus.
Carpiodes.  Catostomus.  Erimvzon. Hvpentelium.
Ictiobus.  Mipytreq|ia. and Moxostoma.
Headwater Sites

The number  of minnow  species is substituted
for the number of sucker species  in headwater
sites.  The number  of sucker  species  decreases
rapidly with declining drainage area (Fig. 8).
While as many as ten different minnow species
have been observed  at locations  with drainage
areas less than 20 miles2.  The number of
minnow  species  generally  correlates with
increased  environmental quality.
Environmental tolerance  is represented   at both
ends of the continuum.   Sensitive species  such
as hornyhead  chub (Nocomis biguttatusl.
bigeye chub  (Hvbopsis  amblops). and  rosyface
shiners (Notropis rubellus) are examples  of
minnow species  which occur  in high quality
headwater  streams. Species  such as creek chub
(Semotilus  atromaculatus)  and  fathead  minnow
             promelas)  are  tolerant  to both
chemical degradation  and stream intermittence.
The number of minnow species  increases  with
increasing drainage area  (Fig. 8).

Wadable River Sites

The number of sucker species,  with the
exception  of the Catostomus  compersnni.
Ictiobus and Carpiodes.  represent  sensitive
species  intolerant  to thermal, siltation, and
toxins stresses. The redhorses  are particularly
important  indicator organisms in rivers. Round-
bodied  suckers include members  of the genera
Cvcleptus.  Hvpentelium. Moxostoma.
Minvtrema. and Erimvzon. These  species  are
effectively sampled with electrofishing  gear and
comprise  a significant component  of  riverine fish
faunas.  Their feeding  and reproductive
requirements  are sensitive to turbidity and
marginal to poor  water quality.  The number  of
species  were not significantly different among
the two ecoregions or between  the three sub-
basins  (Fig. 9).
                                                  25

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Indiana
Table  6. Distribution characteristics
         of Indiana sucker  species
         (family Catostomidae)   in
         the Maumee  River.

                           Large  Rare
Species            Statewide  Rivers Taxa
Cvcleptus elongates
Carpiodes carpio
C. cvprinus
C. velifer
X
X X
X
X
X


X
Erimvzon oblongus       X

Erimvzon sucetta

Hvpeotelium nigricans     X   X
Ictiobus bubalus
I. cvprinellus
I. niger
Lagochila lacera
Minvtrema melanops
^doxostotna anisuruin
M.carinatum
M. duquesnei
M. ervthrurum
M. macrolepidotum
M. valenciennesi
X
X

X
X
X
EXTINCT
X
X

X
X
X


X
X
X
X
X
X
                                           26

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                   Wading/Headwater  Sites
                St.  Joseph    A   St. Marys      +  Maumee
                Drainage          Drainage          Drainage

-------
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-------
Metric 5. Number  of Sensitive   Species   (Headwater  and Wadable  Sites)
Impetus

The number  of sensitive species  metric                  2).
distinguishes between streams of highest
quality. Designation  of too many species as
intolerant  will prevent  this metric from
discriminating among  the highest quality
resources.  Only species  that are highly
intolerant  to a variety of disturbances  were
included in this metric so  it will  respond to
diverse types of perturbations  (Table 7; see
Appendix  A for species-specific  information).

The number  of intolerant taxa is a modification
of the original index  developed  by Ohio EPA
(1987). The metric included moderately
intolerant  species  when sampling at headwater
sites.  This combination is called sensitive
species since few intolerant taxa  are expected.
The moderately intolerant species  meet  most of
the established  criteria of Ohio EPA (1987). An
absence  of these  species  would indicate a
severe  anthropogenic  stress  or loss of habitat.

The criteria for determining  intolerance is based
on the numerical  and graphical analysis of
Ohio's regional data base, Gerking's (1945)
documentation   of historical changes  in the
distribution of Indiana species,  and
supplemental  information from regional
ichthyofaunal texts  (Pflieger  1975; Smith 1979;
Trautman  1981; Becker 1983; Burr and Warren
 1986). Intolerant taxa are those  which decline
with decreasing environmental  quality and
disappear,  as viable populations,  when  the
aquatic environment  degrades  to the "fair"
category  (Karr et al.  1986). The intolerant
species list was divided  into three categories,  all
are included in this metric for scoring:
                                             or
                                                                       or geographically  restricted
                                                           species  (S): species  that are infrequently
                                                           captured  or that have  restricted  ranges;
                                                        3). rare or possibly extirpated  species  (R):
                                                           intolerant  species  that are rarely captured
                                                       or which lack recent  status data.

                                                       Commonly occurring  intolerant  species  made
                                                       up 5-10% of the common  species  in Indiana.
                                                       This was a recommended  guideline of Karr
                                                       (1981) and Karr et al. (1986). Although the
                                                       addition of species designated  as uncommon
                                                       rare sensitive  species  (categories  2 and 3),
                                                       inflates the number  of intolerant species above
                                                       the 10% guideline, nowhere  in the State do  all
                                                       of the species  coexist at the  same  time. In order
                                                       to evaluate streams  in the Large and Great  river
                                                       categories, only the sensitive species  metric will
                                                       be  used until further resolution is possible with
                                                       the addition  of adjacent  ecoregion  sampling.
                                                       Until more sampling  is completed  or
                                                       improvements  in water quality  warrant it, the
                                                       sensitive species   metric (Ohio  EPA 198 1) will be
                                                       used for all headwater  streams  and wadable
                                                       river sites in Indiana.
  1). fvnmmnn  intolerant species  (I): species
     which are intolerant, but are widely
     distributed  in the best  streams  in Indiana;
Headwater and Wadable  Streams and Rivers

The number of intolerant species  increases  with
drainage area among  headwater  and wading
sites (Fig. 10). Intolerant taxa are scarce in the
Huron-Erie Lake Plain and may even decrease
at larger wading sites. In order to provide
meaningful stream  reach comparisons   in
Indiana,  the sensitive species  metric is currently
retained  until further evaluation can  be
completed.
                                                   29

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Indiana
Table  7. List of Indiana fish species   considered   to be sensitive   to a wide
           variety of environmental  disturbances  including  water quality and
         habitat degradation.
Common  Name
Scientific  Name
Common  Name
Scientific Name
Ohio lamprey
Northern brk lamprey
Least brook lamprey
American  brk lamprey

Paddlefish
Goldeye
Mooneye
Redside dace
Streamline  chub
Gravel chub
Speckled chub
Bigeye chub
Pallid shiner
Rosefin shiner
Hornyhead  chub
River chub
Pugnose  shiner
Popeye shiner
Bigeye shiner
Ironcolor shiner
Blackchin shiner
Blacknose  shiner
Sand  shiner
Silver shiner
Rosyface shiner
Silverband  shiner
Weed  shiner
Mimic shiner
Pugnose  minnow
Longnose dace
Blue sucker
Highfin carpsucker
Northern hogsucker
Silver redhorse
River redhorse
Black redhorse
Golden redhorse
Shorthead  redhorse
Greater redhorse
Ichthvomvzon  bdellium Mountain madtom
I. fossor
T-ampetra  aepyptera
L. appendix

Polvodon  spathula
Hiodon alosoides
H. tergisus
            elongatus
E. x-punctata
Extrarius aestivalis
Hvbopsis  amblops
H. amnis
Lvthrurus ardens
Nocomis biguttatus
Notropis anogenus
M- arjojnmuj
N.boops
N. chalvbaeus
N. heterodon
N. heterolepis
N. ludibundis
N. photogenis
N. rubellus
N. shumardi
£f . texanus
N. volucellus
Opsopoeodus   emiliae
Rhinichthys cataractae
Cvcleptus elongates
Carpiodes  velifer
Hvpentelium nigricans
Moxostoma anisurum
M. carinatum
M. duquesnei
M. ervthurum
M. macrolepidotum
M. valenciennesi
Slender  madtom
Stonecat
Brindled  madtom
Freckled  madtom
Northern madtom
Northern cavefish
Southern  cavefish

Northern studfish
Starhead  topminnow

Brook silverside

Rock bass
Longear  sunfish
Smallmouth  bass

Western  sand  darter
Eastern  sand darter
Greenside darter
Rainbow darter
Bluebreast  darter
Harlequin darter
Spotted  darter
Spottail  darter
Tippecanoe  darter
Variegate darter
Banded  darter
Logperch
Channel  darter
Gilt darter
Slenderhead   darter
Dusky darter
Saddleback  darter
Noturus eleutherus
N. exilis
N. flavus
N. miurus
N. nocturnus
N. stigmosus
Amblvopsis spelaea
T. subterraneus

Fundulus catenatus
£• dispar

Labidesthes  sicculus

Ambloplites rupestris
Leoomis megafotis
Micropterus dolomieui

Ammnnrypffl
A. oellucida
Etheostorna
E. caeruleum
S- camurum

E.maculatum
£. squamiceps
E. tippecanoe
E. variatum
E. zonale
Percina caprodes
P. copelandi
P. evides
£• pfaoxocephala
£. sciera
E- vigil
                                                 30

-------
                              l£
             NUMBER OF SENSITIVE SPECIES
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-------
Metric 6. Percent Abundance   of Tolerant  Species
            Sites)
        (Headwater  and  Wadable
Impetus

This metric  is a modification of the original
index metric, the percentage  of green sunfish
(Karr et al. 1986), by Ohio EPA (1989). This
metric detects  a decline in stream  quality from
fair to poor  categories.  The green sunfish,
Lapomig cvanellus. is a species that  is often
present  in moderate numbers  in many Midwest
streams and can become  a dominant member
of the community in cases  of degradation or
poor  water quality. Competitive advantage  in
disturbed  environments enables  the  green
sunfish  to survive and reproduce  even under
perturbed  conditions.  Although the green
sunfish  is widely distributed in the Midwest, it is
most  commonly  collected  in headwater  streams.
This introduces  an inherent bias for moderate  to
large  rivers. Karr et al. (1986) suggested
additional species could be substituted  for the
green sunfish if they responded  in a similar
manner. Several  species in Indiana meet this
criteria of increasing in proportion with
increasing degradation  of stream  quality. This
increase  in the number of tolerant species
increases  the sensitivity of this metric for various
sized  streams  and  rivers. Since different species
have  habitat  requirements  that are correlated
with stream  size, compositional diversity of the
tolerant species  metric does not change  with
         area.

Indiana's tolerant species  are listed in Table 8.
This list is based  on a numerical and  graphical
analysis of Indiana catch  data  and historical
changes  in the distribution of fishes throughout
Indiana (Gerking 1945). Species  listed as
tolerant taxa  exhibit diverse tolerance  to thermal
loadings, siltation, habitat degradation, and
certain toxins (Gammon  1983; Ohio EPA 1989).
Tolerant species were selected  based  on  the
following criteria:

 1) present at poor or fair sites: Based  on
    our data base  of Indiana  collections
    these  species  are commonly  collected at
    sites ranked either fair or poor.

 2) historically increases  i" aKmirfanm-
    Based  on historical collection  information
    (Gerking 1945) these species  increase in
    abundance  and have not indicated  any
    reduction in distribution.

 3) increased  tolerance  to degraded
   conditions:  these species  increased in
    community  dominance  when
    environmental  conditions  shifted  from
    good  to fair or poor environmental
    quality.
Headwater and Wadable  Streams  and Rivers

No relationship was evident for drainage  areas
between  20-1000 miles1  (Fig. 11), nor was there
any relationship  with ecoregion  or sub-basin
apparent  for the Maumee River drainage.
                                                  32

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                                                                           Huron-Erie Lake Plain
Table 8. List of Indiana fish species   considered  to be highly tolerant to a wide
         variety of environmental  disturbances   including  water quality  and
         habitat degradation  for Headwater  and Wadable  River sites.

Tolerant  Species
Common Name                             Scientific Name
Loognose  gar
Shortnose  gar

Gizzard shad

Central mudminnow

Carp
Goldfish
Red shiner
Golden shiner
Bluntnose  minnow
Fathead minnow
Blacknose  dace
Creek chub

River carpsucker
Quillback
Smallmouth buffalo
Bigmouth  buffalo
White sucker

Channel catfish
Ftathead catfish
Yellow bullhead
Brown bullhead

Eastern  banded killifish

Freshwater drum

White bass

Green  sunfish
Lepisosteus  osseus
L. platostomus

          cepedia^um

Umbra limi

Cvprinus carpio
Carrasius auratus
Cyprinella lutrensis
Nntemigoniis  crvsoleucas
P. promelas
           atratulus
Carpiodes  cvprinus
C. carpio
Ictiobus bubalus
I. cvprinellus
Catostomus
Ictalurus punctatus
Pvlodictis olivaris
Amieums natalis
A. melas

Fundulus diaphanus  diaphanus

Aplodinotus grunniens

Morone chrvsops

Lepomis cvanellus
                                               33

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                   Wading/Headwater   Sites
                                                                                     i
                                                                                     9

                                                                                     9
                St.  Joseph
                Drainage
           st. Marys
           Drainage
*   Maumee
    Drainage
      1OO
   O
A Q o
-.A A £|£ f
	 .sr?*.*.! 	
o + r +
+ 0* ^ 0Q
I O
: ' o
A+ I
! ° o
'. o '
I
$1 A;

o •
3o \
A
5
         0.1
1             10           10O

  DRANAGE AREA  (SQ. Ml)
              100O
Figure 11  Maximum species richness lines for determining trends in the proportion of tolerant
        species with increasing drainage area for the Maumee River drainage.

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                                                                               Huron-Erie Lake Plain
Metric  7.  Proportion  of Omnivores  (Headwater  and Wadable  Rivers)
Impetus

The definition of an omnivore follows that of
Kan (1981) and Karr et ol. (1986), which
requires  species to consume  significant
quantities of both  plant and animal materials
(including detritus) and have the ability (usually
indicated by  the presence  of a long gut and
dark peritoneum)  to utilize both.  Omnivores are
species  whose diets include at least 25% plant
and 25% animal foods. Fishes which do not
feed on plants but on  a variety of animal
material  are not considered  omnivores.
Dominance of omnivores  suggests  specific
components  of the food base are less  reliable,
increasing  the success  of more opportunistic
species.  Specialized filter-feeders are not
included in this metric after Ohio EPA (1989)
since these species  are sensitive to
environmental  degradation,  e.g.  paddlefish,
Polvodon spatnula and lamprey  ammocoetes,
I^flyfira and Ichthvomvzon.  Facultative
species   which shift diet due to degraded
environmental  conditions  are also not
considered omnivores, e.g. Semotilus
atromaculatus  and Rhinichthys  atratulus. This
metric evaluates  the intermediate to low
categories  of environmental  quality (Table 9;  see
Appendix B  for species-specific   feeding guild
classification).

Headwater and Wadable Streams  and Rivers

Due to minor changes  in omnivore
classification, only those  species  which
consistently  feed  as omnivores  were included in
our analysis.  These values  differ from the
omnivore  percentages   of Karr et ol. (1986) but
resemble Ohio EPA's (1987) classification.  No
relationship  with drainage area was found for
headwater or wadable  stream and river sites
(Fig. 12). The lack of a drainage area pattern is
anticipated since  degraded  habitats  are not
 exclusive  to any particular  size waterbody.
Table  9.  List of Indiana fish  species
         considered   to  be  omnivores.
Common  Name
                       Scientific Name
Gizzard shad


Threadfin  shad

Central  mudminnow

Goldfish

Grass carp


Carp

Cypress minnow

Miss, silvery minnow

Silver carp


Black carp


Bluntnose  minnow

Fathead  minnow

Bullhead  minnow

Rudd


River carpsucker

Quillback

Highfin carpsucker

White sucker
                       Dorosoma
                       cepedianum

                       D. petenense

                       Umbra  limi

                       Carassius  auratus

                       Ctenopharyngodon
                       idella

                       Cvprinus carpio

                       Hybognathus  hayi

                       H. nuchalis
                       molitrix

                       Mvlopharyngodon
                       piceus

                       Pimephales  notatus

                       P. promelas

                       P. vigilax

                       Scardinius
                       ervthropbtfaalmus

                       Carpiodes  carpio

                       C. cvprinus

                       C. velifer

                       Catostomus
                                                                              commersom
                                                  35

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                   Wading/Headwater  Sites
            o   St. Joseph
                Drainage
      1OO
  CO


  >
St. Marys     +  Maumee
Drainage          Drainage
                       1             10          10O

                         DRAINAGE AREA (SO. Ml)
                            1000
Figure 12. Maximum species richness lines ibr determining trends in the proportion of omaivoies
       with increasing drainage area for the Maumee River drainage.            '

-------
                                                                                Huron-Erie Lake Plain
Metric  8.  Proportion  of Insectivores   (Headwater  and Wadable  Sites)
Impetus

The proportion of insectivores is a modification
of KMT et al. 's (1986) original metric, i.e.
proportion  of insectivorous cyprinidae.  This
metric is intended  to respond  to a depletion  of
the benthic macroinveitebrate  community  which
comprises  the primary food base  for most
insectivorous  fishes. As disturbance  increases,
the diversity of insect larvae decreases,
triggering an increase  in the omnivorous trophic
level. Thus, this metric varies  inversely  with
metric 7  with increased  environmental
degradation.  The inclusion of all insectivorous
species  was based on the observation  that all
regions of Indiana do not possess  high
proportions  of insectivorous cyprinids in high
quality streams,  e.g. Central Corn Belt  Plain  and
Interior Plateau  ecoregions.  This metric was
recalibrated following the recommendation  of
Karr et al, (1986; see Appendix  B for species-
specific trophic  level classifications).
Headwater and Wadable Streams  and Rivers

Insectivorous  species are an important link in
transfering  energy between  lower trophic levels
to keystone  predator  species.   Species
designations  generally conforms  to mat
provided in Karr et al. (1986), however, 1 concur
with Ohio EPA in the elimination of the
opportunistic  feeding creek  chub, Semotilus
atromaculatus.  and blacknose  dace,
atratulus. from the insectivore designation.
Leonard and Orth (1986) felt that  the current
trophic definitions of Karr et al. (1986) were
rather arbitrary since they observed  a negative
correlation  between  insectivores and biotic
integrity in a West Virginia stream. Plots of the
MSR lines showed no relationship existed
between drainage  area  and proportion  of
insectivorous fishes  in either ecoregion  or sub-
basin in the Maumee  River drainage (Fig. 13).
                                                 37

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00
                                     Wading/Headwater  Sites

rrt
TIVOREJ
U



1OO
75
5O
oc
25
0
o St. Joseph A St. Marys
Drainage Drainage
I
+ + i
: *' o
0 +
; i
/\ I 4*
* -i o
0 ^ u o *
© o i o
+O A ' A^O
* A* ^\ O
^Br yNQ i T
A iA
A ^ • 1
"§* A
1 1 10
+ Maumee
Drainage
O
'o°° 5
3
1
1OO

A
o •
A '
^ A
o!
°A
1O
                                           DRAINAGE  AREA (SO.  Ml)
                  Figure 13. Maximum species richness lines for determining trends in the proportion of insectivores
                          with increasing drainage area for the Maumee River drainage.

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                                                                                Huron-Erie Lake Plain
Metric 9. Proportion  of Pioneer  Species  (Headwater  Streams)
            Proportion  of Carnivores  (Wadable  Rivers)
Impetus

Karr (1981) developed  the carnivore metric to
measure  community integrity in the upper
trophic  levels of the fish community. It is only in
high quality environments  that upper trophic
levels are able to flourish. This metric  includes
individuals of species in which the adults are
predominantly  piscivores,  although some may
feed on invertebrates and  fish as larvae  or
juveniles. Species  which are opportunistic do
not fit into this metric, e.g. creek  chub or
channel catfish, Ictalurus  punctatus  (Karr et al.
1986; Ohio EPA 1987). Karr et al. (1986)
suggest mat some  members  of this group may
feed extensively on crayfish and various
vertebrates, e.g. frogs. Species-specific
classifications are included in Appendix  B and
include piscivores  (P) and carnivores  (C).

Headwater Streams

Carnivores are generally not abundant in
headwater  streams.  An alternate  metric  was
developed  by Ohio EPA (1987) to determine the
permanence  of the  stream  habitat. Smith (1971)
identified a signature assemblage  of small
stream  species  which he termed "pioneer
species"  (Table 10). These  are species which
are the first to colonize sections of headwater
streams  after desiccation.  These pioneer
species  predominate in unstable  environments
affected  by anthropogenic   stresses  and
temporal  desiccation. A high proportion  of
pioneer species indicates  an environment
temporally unavailable or stressed.  The  metric
does  decreases  with increases in drainage  area
(Fig. 14).

Wadable Sites

Karr (1981) suggested  that the proportion of
carnivores should be a reflection of drainage
Table 10. List of Indiana fish species
          considered  to be indicators  of
          temporally unavailable  or
          desiccated   habitats  (Larimore and
          Smith 1963; Smith 1971).

Pioneer Species
         Name         Scientific Name
Central stoneroller


Largescale  stoneroller


Silverjaw minnow

Bluntnose  minnow

Fathead minnow

Creek chub


Creek chubsucker

Lake chubsucker

Green sunfish

Johnny darter

Orangetbroat  darter
                       oligolepis

                       Ericvmba buccata

                       Piifiaptffllftg  notatllS

                       Pimephales  promelas

                       Semotilus
                       atromaculatus

                       Erimvzon  obioneus

                       Erimvzon sucetta

                             H§ cvanellus

                                   nigrum
                       Ethepstnma  spectabile
area. Such a correlation  in streams greater than
20 miles2 was not found  by Ohio EPA or
previous  ecoregion  studies  (Simon,  1991). A
drainage  area relationship was observed
between  the  sub-basins  and increasing drainage
area in the Maumee  River drainage.  The
proportion  of carnivores  from the current  data
base was considerably higher than that
approximated  in Karr et al.'s (1986) original
numbers  (Fig. 15).
                                                  39

-------
                   Wading/Headwater  Sites
St. Joseph
Drainage
                     A   St. Marys
                         Drainage
                                                 +   Maumee
                                                     Drainage
      100
  
  g   75
0.1
                                                         10
                         DRAINAGE AREA  (SQ. Ml)
Figure 14. Maximum species richness lines for determining trends in the proportion of pioneer
        species with increasing drainage area for the Maumee River drainage.

-------
                   Wading/Headwater   Sites
    to
       20
St. Joseph
Drainage
                          st. Marys
                          Drainage

0 "-«—«Q  v'»* '
 20
                           o o
                          -rfi—
                                                +   Maumee
                                                    Drainage
                             -e-1-
                               100

                         DRAINAGE  AREA (SO. Ml)
                                              1000
Figure 15. Maximum species richness lines for determining trends in the proportion of carnivores
        \vithincreasingdrainageareafbrtheMauraeeRiverdrainage.

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Indiana  Ecnresion
Metric 10. Number of Individuals  in a Sample  (Headwater  and Wadable  Sites)
Impetus

This metric evaluates population density and is
expressed  as catch  per unit of effort. Effort is
expressed  by the relative number of individuals
per length of reach  sampled,  per unit of area
sampled,  or  per unit time spent depending  on
the gear used.  Karr et al. (1986) suggest that
this metric is most sensitive  at  intermediate  to
low ends  of  the sensitivity continuum. When low
numbers  of individuals are observed  the normal
trophic relationships  are generally  disturbed.
Because  of this effect,  scoring  adjustments  are
encouraged   for sites in which less than 50
individuals are collected (see next section  for
details). As integrity increases,  total abundance
increases  and  becomes  more variable only
depending on  the level of energy and other
natural chemical  factors limiting production.
Under certain circumstances,  e.g.
channelization, increases in the abundance  of
tolerant fishes  can be observed  (Ohio EPA
1987). Lyons (1992) found that abundance,
excluding tolerant species,  was greatest at fair
quality sites  in Wisconsin warmwater  streams
and  lower at sites classified  as excellent. In this
study,
catch  per unit effort was determined  based
on the total number  of individuals collected  per
15 times the channel  width without  modification
for tolerant taxa. The level of effort sampled
within a reach was 50 m if the stream was < 3.4
m wide or 100 m minimum distance  if the
stream was > 3.4 m wide. A maximum  distance
of 1000 m was sampled  for stream  widths >
66.7 m.  Each shocking  run was conducted  with
a standardized  effort of 30 minutes  of sampling
per shoreline  in 1000 m  sites  and 15 minutes
per shoreline  at 500 m sites including both
shorelines.
Headwater  and Wadable  Streams  and Rivers

A drainage area-dependent   relationship was
observed  for the Maumee  River drainage  (Fig.
16).

If fewer than  50 fish are collected  during a
sampling  event, alternate  scoring  procedures
are required (see next section  for details).  Even
at the river reach with the  smallest drainage
area I was able to collect a minimum of 100
fish.
                                                  42

-------
                   Wading/Headwater  Sites
      8OO
  U
St. Joseph
Drainage
St. Marys
Drainage
                                               *   Maumee
                                                   Drainage
                      1           1O         10O

                         DRAINAGE AREA  (SQ. M)
                      1OOO
Figure 16. Maximum species richness lines for determining trends in the catch per unit of effort
        with increasing drainage area for the Maumee River drainage.

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Metric 11. Proportion  of Individuals as Simple  Lithophilic Spawners
          (Headwater  and  Wadable  Rivers)
Impetus

Ohio EPA (1987) replaced the original index
metric, proportion of hybrids  (Karr et al. 1986),
with this metric. The hybrid metric was
abandoned  since the original intent of the metric
was to assess  the extent to which degradation
has altered  reproductive  isolation among
species.  Difficulties of identification, lack of
occurrence  in headwater  and impacted  streams,
and presence  in high quality streams  among
certain taxa, e.g., cyprinids and centrarchids,
caused  a lack of sensitivity for the hybrid metric.

Spawning guilds have been  shown  to be
affected  by  habitat  quality (Balon 1975;
Berkman  and  Rabeni 1987) and  have been
suggested  as  an alternative metric (Angermeier
and Karr 1986).  Reproductive attributes of
simple spawning behavior requires  clean gravel
or cobble for success  (i.e. lithophilous) and are
the most  environmentally sensitive (Ohio EPA
1987). Simple lithophils broadcast  eggs which
then come into  contact  with the substrate.  Eggs
develop  in the interstitial spaces  between sand,
gravel, and  cobble  substrates   without parental
care. Berkman  and Rabeni (1987) observed  an
inverse  correlation  between simple  lithophilic
spawners  and the proportion  of silt in streams.
Historically, some simple lithophilic spawners
have
experienced   significant range reductions  due  to
increased  silt loads in streams.  Some simple
lithophils do  not require clean  substrates  for
reproduction. Larvae of these species are
buoyant, adhesive,  or possess  fast developing
eggs with phototactic  larvae which have  minimal
contact  with the substrate  (Balon 1975) and are
not included  in the above designation.  Simple
lithophils are sensitive to environmental
disturbance,   particularly siltation. Designated
lithophilic species  are included in Table 11 (see
Appendix  C for species-specific   ratings).
Headwater and Wadable  Streams  and Rivers

A relationship  with drainage  area  was observed
at stream  and river sites for the proportion of
lithophilic species  in the Maumee  River drainage
(Fig. 17). Scoring  was completed  using the
trisection method  of Fausch  et al. (1984). The
lack of an increased  relationship  in the largest
Maumee River drainage  reaches  was  nought  to
be a reflection of degraded  condtions.  Best
professional  judgement  was used in evaluating
this metric. Simple lithophils are major
components  of fish communities  indicating the
importance of clean  gravel and  cobble
substrates.
                                                  44

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                                                                               Huron-Erie Lake Plain
Table  11. List  of Indiana  species   considered  to be  simple  lithophilic  spawners.

Simple  Lithophils
Common Name
Scientific name
Common  Name
Scientific Name
Paddlefish
Lake sturgeon
Shovehiose  sturgeon

Redside dace
Lake chub
Streamline  chub
Gravel chub
Cypress minnow
Mississippi
silvery minnow
Bigeye chub
Pallid shiner
Striped shiner
Common shiner
Rosefin shiner
Popeye  shiner
River shiner
Bigeye shiner
Silver shiner
Rosyface shiner
Silverband  shiner
Southn redbelly dace
Blacknose  dace
Longnose   dace

Blue sucker
Longnose   sucker
White sucker
Northern hogsuckei
Polyodon  spatula
Acipenser fulvescens
Scaphirfavnchus  platorvnchus

Clinostomus elongatus
Couesius  plumbeus
Erimvstax dissimilis
E- x-punctata
Hvbognathus havi

JJ. nuchalis
Hvbopsis  amblops
H. amnis
Luxilus chrvsocephalus
L. comutus
Lvthrurus ardens
N. ariommus
N. blennius
N. boons
IN. photoyenis
N. rubellus
M. shumardi
Phfmnus  ervthrogaster
Rhjnichthyg  atratulus
R. cataractae

Cvcleptus elongatus
Catostomus  catostomus
f* JXJ-L«V> «vt.a. m-dj-Ln *
Hvpentilium nigricans
Spotted  sucker
Silver redhorse
River redhorse
Black redhorse
Golden redhorse
Shorthead redhorse
Greater redhorse

Burbot
Western sand  darter
Eastern  sand darter
Rainbow darter
Bluebreast  darter
Orangethroat darter
Tippecanoe  darter
Variegate  darter
Crystal darter
Logperch
Channel darter
Gilt darter
Blackside  darter
Slenderhead darter
Dusky darter
River darter
Saddleback  darter
Sauger
Walleye
Minvtrema melanops
Moxostoma anisurum
M.carinatum
M. duquesnei
M. ervthrurum
M. """Tolepidotuni
M. valerfcjenpesi

Lota lota
Amnw.ryrrfa clara
A. pellucida
F.thenstnma caeruleum
E. camurum
E. spectabile
E. tippecanoe
E. variatum
Crvstallaria asprella
Percina caorodes
P. copelandi
P. evides
P. maculata
P. phoxocephala
£. sciera

£. vigil
Stizostedion  canadense
S. vitreum
                                                 45

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                   Wading/Headwater   Sites
                St.  Joseph
                Drainage
      100
       75
       50
       25
         0.1
           St. Marys
           Drainage
Maumee
Drainage
                                                   .-'"   5
1            10           100

  DRAINAGE AREA (SQ  Ml)
          1000
Figure 17. Maximum species richness lines for determining trends in the proportion of simple
        lithophil species with increasing drainage area for the Maumee River drainage: -

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Metric 12. Proportion  of Individuals  with Deformities,  Eroded  Fins,  Lesions,
             and Tumors  (Headwater  and Wadable  Sites)
Impetus

This metric evaluates the individual condition of
fish based  on the percent  occurrence  of
external anomalies.  DELTcorresponds  to the
percent of diseased  fish in Karr's (1981) original
index. Studies  of fish populations  indicate that
anomalies  are  either absent  or occur  at very low
rates  naturally, but reach higher percentages  at
impacted  sites (Mills# al,  1966; Bern and Au
1981; Baumann et al. 1987). Common  causes
for deformities, eroded  fins, lesions, and  tumors
are a result of bacterial, fungal, viral, and
parasitic infections;  neoplastic  diseases;  and
chemicals  (Allison « al. 1977; Post 1983; Ohio
EPA 1987).  An increase  in the frequency  of
occurrence  of these anomalies  is an indication
of physical stress due to environmental
degradation,  chemical  pollutants, overcrowding,
improper  diet, excessive  siltation, and other
perturbations.  The presence of black  spot is not
included in the above analyses since  infestation
varies in degree  and is a function  of the
presence  of snails,  thus it is not solely related to
environmental  degradation  (Allison et al.  1977;
Bern and Au 1981). Whittier* al. (1987)
showed no relationship  between Ohio stream
quality and black spot. Other  parasites are also
excluded  due  to the lack of a consistent
relationship  with environmental degradation.
In Ohio and in the current  study,  the highest
incidence  of deformities, eroded  fins, lesions,
and tumors  occurred  in fish communities
downstream from dischargers  of industrial and
municipal wastewater,  and areas  subjected  to
the  intermittent stresses  from combined sewers
and urban  runoff. Leonard and Orth (1986)
found this metric to correspond  to increased
degradation  in streams  in  West Virginia. Karr et
al. (1986) observed  this metric to be most
sensitive at the lowest ranges  of the Index of
Biotic Integrity.
Headwater  and Wadable Streams and Rivers

The scoring criteria used for this metric  follows
Ohio EPA (1987) and was developed  by
analyzing wading  data. According to Ohio
protocols, if a single fish in a sample  of less
than 200 fish was captured  with anomalies  this
would have been  enough to exceed  the
established  criterion. Ohio EPA scoring
modifications  enable a single diseased  fish to
be  present  at a site to score a "5"and two fish
at a site to  score  a "3"when less than 200
individuals are collected  (Fig.  18).
                                                   47

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           Wading/Headwater  Sites
15
1O
5
O
O.
Max
O St. Joseph & St. Marys + Maumee
Drainage Drainage Drainage
O
A
, 	 p. 	
+ ° *"
	 , . . — "-^""jrfhTTitvi -m • fi rt o -o- • -iflnfe*
_ 1
_ 3
L. 5
1 1 1O 10O 1OOO
DRAINAGE AREA (Sa Ml)
imum species richness liaes for determining treads in the proportion of diseased,
eroded fins, lesions, and tumors (DELT) with increasing drainage area for the Maumee
River drainage.

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                                                                                 Huron-Erie Lake Plain
Scoring  Modifications
Samples  with extremely low numbers in the
catch can present a scoring problem in some of
the proportional metrics unless adjustments  are
made to reduce the possibility of bias towards
higher scoring  of degraded sites. Aquatic
habitats  impacted  by anthropogenic
disturbances  may exhibit a disruption in the
food base and the  sample will reflect very few
individuals. At such low population  sizes the
normal structure  of the community  is
unpredictable  (Ohio EPA 1987).  Based  on Ohio
EPA experiences,  the  proportion of omnivores,
insectivorous  fishes, and percent  individuals
affected  by anomalies  do  not always match
expected  trends at these sample  sizes.  Although
scores are expected to deviate strongly from
those of high quality areas, this is not always
observed. Rather, at these times the opposite
deviation  of metric score is achieved due to low
numbers  of individuals or absence   of certain
taxa.

Scoring very degraded  sites without modifying
scoring criteria for the proportional  metrics can
overestimate  the total index score  for these
sites. The following scoring modifications
proposed  by Ohio  EPA (1987) were adopted  for
evaluating Indiana sites  with low numbers of
individuals.

Proportion of omnivores for headwater  streams
and wadable  river sites a score of "l"is
assigned   if less  than 50 total  individuals are
collected. When less  than 150 individuals are
collected, but are dominated  (>50%)by such
species  as creek chub  and blacknose dace,  a
"1'can be assigned  when dominated by
generalise feeders.  This is left up to the
biologists best professional judgement.
Proportion of insectivores  is scored  a "1'when
a high proportion  of insectivores  are observed
and less than 50 individuals are collected.  At
sites with less than  150 individuals, this metric
can be  scored  "1 "ifthe community  was
dominated  (>50%)by either striped shiner,
common shiner, or spotfin  shiner. These
species  can act as functional omnivores under
certain conditions  (Angermeier  and Karr 1986).

Proportion of top carnivores metric should be
scored a "I'when dominated by high numbers
(> 50%) of grass pickerel  in impacted wading
areas.

Proportion  of sample lithophils always  scores  a
Tat sites with less  than  50 total individuals.
Based  on Ohio EPA data  (1987) this is rarely
different from the metric score  without the
adjustment.

Proportion  of individuals with deformities.
erosion, lesjofys  and tumor anomalies  is scored
a Twhen less than 50 individuals are
collected.  A high proportion of young  fishes
may also be  sufficient reason to score a "1"
since  they  will not have had sufficient thru, 10
develop anomalies  from exposure  to chemical
contaminants.

Proportion of pioneer species  is scored a  "l"at
headwater  site if less than 50 individuals are
collected at drainage areas greater than 8 miles2
or 25 individuals at drainage areas  less than 8
miles2.
No scoring adjustments  are necessary
proportion  of tolerant species.
for
                                                  49

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RESULTS  AND DISCUSSION

  Maumee River Drainage

Species  Composition:  A total of 21 sites were
sampled in die Maumee  River drainage during
1991. A total of 54 species  were collected
(Table  12) and were numerically dominated by
cyprinid, catostomid, and centrarchid  species.

The fish assemblages  of the  Maumee  River
ranges  from a low of no  fish (score of 0; 1 site)
to good-excellent  (score  of 55; 1 site) based  on
the Index of Biotic Integrity scoring criteria.
Increasing  biological integrity was observed
from upstream  to downstream,  however,
declining  conditions  were observed in the
headwaters  of the  minor  tributaries. The Index
of Biotic Integrity scores  of the sites
approximated  a normal curve with a mode
classification of poor. The frequency distribution
for each of the IBI community categories for the
Maumee River drainage stations (21) follows:
good-excellent  4.8% (1 station); good  9.5% (2
stations);  fair-good 4.8%  (1 station);  fair 4.8% (1
station); poor-fair 19.0% (4 stations); poor
28.6% (6 stations);  very poor-poor 9.5% (2
stations);  very poor 14.3% (3 stations)  and no
fish 4.8% (1 station). The sites with tow
biological integrity  were primarily headwater and
mid-reach rivers. The highest biotic integrity was
associated  with the Maumee  River, at the SR 24
bridge in New Haven (IBI = 55). This River
segment deserves  protection  to ensure  that the
quality of the resource  continues. Some of the
northern tributaries are intermittent and were dry
during our investigations  in 1991.

Several  species  collected  only in the Maumee
River during this  study  included: emerald shiner
Notropis atfaerinoides.  river shiner  Notropis
hlennius. and  flathead catfish Pvlodictis olivaris.
Of special interest was  the  collection of the river
redhorse Moxostoma carinatum. largescale
stoneroller  Campostoma  oligolepis. and
 smallmouth bass  Micropterus  dolomieui
 Moxostoma carinatum  is considered  state
 threatened.  The capture of Canjgostoma
 oligolepis is the first record  for northeastern
 Indiana.  Species  such as the emerald  shiner
 and river shiner are considered  large river
 species.  Several  Micropterus dolomieui (N = 3)
 were collected from the Maumee  River at the
 upper two stations,  however, none  were
 observed  at Bull Rapids and the Ohio state line.

 Species  Trends:  Round-bodied suckers,
 minnows, and darters  are good indicator  taxa
 revealing  good to exceptional  biotic integrity.
 The Maumee  River possessed  a high number of
 round-bodied  suckers,  but limited numbers  of
 minnows  and  darters.

 Eight species  of suckers  were collected from
 the Maumee River drainage.  Round-bodied
 suckers are considered   the most  sensitive of the
 Catostomidae  species.  A total of 5  species,
 excluding the species  Catostomus   commersoni
 which tends to be a ubiquitous species  found in
 a variety of habitats, represented  round-bodied
 suckers.  Four species  of redhorse  and  the
 northern  hogsucker  are  regularly  represented  in
 catches from  the Maumee River and the larger
 tributaries. These species are insectivores  and
 are highly intolerant to thermal  pollution
 (Gammon  1976).

 The minnows  are a diverse  group of North
 American  fish with close to 200 recognized
 species. Seventeen  species  of the family
 Cyprinidae were collected  from the  Maumee
 portion of the  drainage.  Less than half (45%) of
 the species  are considered  to represent good-
 fair biotic  integrity (Karr et al. 1986). Many
 (40%) of the species are representative  of
pioneering  taxa which colonize  recently
disturbed  or water limited stations (Ohio EPA
 1989). The trophic composition  of the species
showed 25% of the species  are omnivores.
Omnivores can utilize a  greater  proportion  of
the resource,
                                                  50

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                                                                             Huron-Erie Lake Plain
Table  12.  Species   list of taxa collected   in the Maumee River drainage:
             Maumee,  St.  Marys', and St. Joseph   River drainages,  Indiana,
             during 1991.

                                                                Drainage
                                                      St. Marys St. Joseph  Maumee
Leptsosteif bnnes  - gars
  Lepisosteidae -gars
L. osseus  Linnaeus, longnose gar                                    XX

Amiiformes - bowfin
  ATiid<"" • bowfin
Amia calva Linnaeus, bowfin                                         X

Clupeifonnes  - herring, shad
  Cluepidae -herring
Dorosoma cepedianum   (Lesueur), gizzard shad                 XXX

Sahnoniformes - pike and mudmianows
  Eaocidae  -pikes
Esox 
-------
 Table  12. (Continued).
Drainage

Rhinichthvs atratulus (Agassiz), blacknose dace
Semotilus atmmaculatus (Mi tchill), creek chub
CfltO^Qiriiflfly - suckers and buffalo
Carpiodes carpio (Rafinesque), river carpsucker
£. cvprinus (Lesueur), quillback
C. velifer (Rafinesque), highfin carpsucker
Catostomus commersoni Lacepede. white sucker
Hvpentelium nigricans (Lesueur), northern hogsucker
Ictiobus bubalus (Rafinesque), smallmouth buffalo
Minvtrema melanops (Rafinesque), spotted sucker
Moxostoma anisurum (Rafinesque), silver redhorse
M. carinatum (Cope), river redhorse
M. duquesnei (Lesueur), black redhorse
M. ervthunun (Rafinesque), golden redhorse
M. macrolepidotum (Lesueur), shorthead redhorse
M. valenciennesi Jordan, greater redhorse
Siluriformes - bullhead and catfish
Ictaluridae - bullhead and catfish
Ameiurus melas (Rafinesque), black bullhead
A., natalis (Lesueur), yellow bullhead
Ictalurus punctatus (Rafinesque), channel catfish
Noturus flavus Rafinesque, stonecat
fi. gvrinus (Mitchill), tadpole madtom
H. mjufus Jordan, brindled madtom
Pvlodictis olivaris (Rafinesque), flathead catfish
St. Marys
X
X

X
X
X
X
X
X

X
X

X
X
X



X
X
X
X


St. Joseph
X
X


X
X
x
X
X
X
X

X
X

X


X
X
X
X

X

Maumee

X


X
X
X




X
X
X
X



X
X
X
X
X
X
X
Atheriniformes  - topminnows,  silversides
  Fundulidae  - topminnows
Fundulus  notatus (Rafinesque),  blackstripe topminnow          X              X
  Athff"Mlh?  • silversides
Labidesthes  sicculus  (Cope), brook silverside                          X

Perrifonnes  - basses,  sunfish, perch, darters
  Centrarchidae  - black bass and  sunfish
Ambioplites rupestris  (Rafinesque),  rock bass                           X       X
Lepomis cvanellus Rafinesque,  green  sunfish                   XXX
L. gibbosus  (Linnaeus), pumpkinseed                           XXX
L. pulosus (Cuvier), warmouth                                         X
If. humilis (Girard), orangespotted  sunfish                      X              X
L. macrochirus  Rafinesque,  bluegill                            XXX
L. meyalotis  (Rafinesque), longear  sunfish                      XXX
L. microlophus  (Gunther), redear sunfish                       X
                                                52

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                                                                                 Huron-Erie Lake Plain
Table  12.   (Continued)
                                                             Drainage
Centrarchidae - black bass  and sunfish  (continued)
Micropterus dolomieui  Lacepede, smallmouth bass
M-ab&fiides  (Lacepede),  largemouth  bass
Pomoxis a^nularis  Rafinesque,  white crappie
£. niyromaculatus   (Lesueur), black  crappie

  Percidae  -perch and darters
             peltucida  Agassiz,  eastern sand  darter
                        Rafinesque,  greenside  darter
                                                         St. Marys  St. Joseph  Maumee
£. flabellare Rafinesque, fantail darter
E. nigrum Rafinesque, johnny darter
E. spectabile  (Agassiz),  orangethroat  darter
Percina caprodes  (Rafinesque),  logperch
£. maculata (Girard), blackside darter
£. pfaoxocephala  (Nelson), slenderhead  darter
Stizostedion  canadense  (Smith), sauger
§. vitreum (Mitchill), walleye

   Sciaenidae  - drum
Anlodinotus  grunniens  Rafinesque,  freshwater  drum

   Cottidae - sculpins
Cottus bairdi Girard, mottled sculpin
        X
        X
        X
        X
        X
        X

        X
        X
X
X
X
X
X
X
X
X

X
X
X
X
X
X
X
X
X
X
X
X
X
                        X
Total Number of Species
        48
58
54     =75
however,  tend to dominate when the habitat is
degraded  and resources  are unpredictable.

The darters are a group  of small, benthic
insecrivores which require  high dissolved
oxygen conditions and clean  substrates  for
reproduction  (Page 1983; Kuehne and Harbour
1983). The  darters have  close  to 150 recognized
species.  Many of the  species  exhibit simple
lithophilic modes  of reproduction,  while a few
species have  evolved more complex
reproductive  behaviors.  A total of 6 darter
species were  collected from the Maumee River
basin. This is significantly below what is
expected  for  the mainstem of the River based
on historical data,  however, may be
representative  of the  tributaries. The eastern
         pellucida was  once
sand darter
found in the basin (Kirsch 1895; Gerking  1945)
but has been  extirpated  from the Maumee River
in Indiana  (Simon 1993). No specimens were
collected  from the Maumee River or minor
tributaries  during the current  investigation. The
darter species  collected  from the Maumee River
represent  the least  reproductively specialized
species  of the 28 species  of darters found within
Indiana and are typical of the fair category of
biotic integrity.

An important  consideration  when evaluating
trends  in the Maumee River is to recognize that
virtually no ideal reference sites exist. Even the
best site immediately upstream  of the Fort
Wayne  outfall was perturbed  by diffuse nonpoint
                                                   53

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Indiana
source  impacts  from the city and was not truly
representative of unimpacted  conditions. Thus,
none  of the sites in the Maumee River were
considered  excellent resource  waters.

  St.  Joseph  River Drajnage

Species  Composition:  A total of 33 wading and
boat sites were  sampled in the St. Joseph River
drainage during  1991.  This drainage  possessed
the most diverse fish community with 58
species (Table 12). Numerically the subdrainage
was dominated  by cyprinids, centrarchid,  and
catostomid  species.  The tributaries  of the St.
Joseph  River, including Fish Creek  and  Cedar
Creek have extremely  diverse  fish communities
composed  of cyprinids, darters,  and
catostomids.   The mainstem St. Joseph  River
scored  the highest IBI at Johnny  Appleseed
park of all sites  sampled during  1991.

The fish community  assemblage  of the  St.
Joseph  River drainage  ranged  from a low of
very poor (score of  14; 3 stations) to good-
excellent (score  of 57; 1 station) based  on IBI
scoring criteria. The biotic integrity  of the St.
Joseph  River varied  with increasing  drainage
area.  Stations above Fort Wayne scored
considerably   poorer  than  the  furthest
downstream  site at Johnny  Appleseed  park.
Like the Maumee River, the IBI  scores  of the
St.  Joseph  River drainage  approximated  a
normal distribution with respect  to biotic
integrity classification.  The biological integrity
rating had  a mode classification  of poor. The
frequency  distribution  for the  St. Joseph  River
stations (33) within each IBI classification
follows: good-excellent   3.0% (1 station); good
6.1% (2 stations); fair-good 6.1% (2 stations);
fair 12.1% (4 stations);  fair-poor 18.2% (6
stations);  poor 39.4% (12 stations);  poor-very
poor  6.1% (2 station);  very poor 9.1% (3
stations).

Fish were collected  at  all sites in the St. Joseph
River drainage.  Sites which had  low index
values were  primarily attributed to non-point
sources (e.g., agriculture). Exceptional  streams
sites  in the St. Joseph  River drainage include
the St. Joseph River at Johnny  Appleseed park
(IBI=57) and below Cedarville Reservoir
(IBI=45), and direct tributaries such as Fish
Creek and Cedar  Creek. Stations  sampled  in the
lower portions  of each  of these  tributaries had
good  biological integrity, however, the
headwaters  of these streams  are degraded  and
need  remediation  to preserve  biodiversity and
biological integrity.

Unique  species  collected  in the St. Joseph  River
include  bowfin Amia calva. northern pike Esox
lucius. steelcolor  shiner Cvprinella whipplei.
hornyhead chub Nocomis bifuttatus.  pugnose
shiner Notropis anogenus.  rosyface  shiner
Notropis rubellus. spotted sucker  Minvtrema
melanops.  brook  silverside Labidesthes
sicculus. warmouth  Lepomis  pulosus.  eastern
sand  darter Ammocrvpta oellucida. slenderhead
darter Percina  phoxocephala.   and mottled
sculpin  Cottus  bairdi. Many of these species  are
sensitive to low dissolved  oxygen, siltation, and
degraded  habitat. This study  indicated that they
may have been reduced  to low population
densities  or extirpated  from the remainder  of the
Maumee River drainage.  In addition, three  State
threatened  species were collected during the
study. The greater redhorse  Moxostoma
valenciennesi.  a state  threatened  species,  was
captured  at several  stations. The eastern  sand
darter was collected at a single location
immediately  adjacent to Johnny Appleseed park
(Simon  1993).  This station is immediately below
the last of a  series of dams which serve as
sediment  traps  removing much  of the sediment
bedload  from the water column. Although the
species  benefits  from the presence of the dam,
the dam also blocks any future  migration
potential upstream.  The pugnose  shiner was
also collected  from a single location.  This
species  typically inhabits lakes and is rarely
collected  from riverine sites. This is the first
collection of this species  from northeastern
Indiana  in over 50 years  (Gerking  1945).
Previously, the species  was only known from
Hamilton Lake, Steuben County, in the St.
Joseph  drainage.  The largescale  stoneroller was
also documented  from the St. Joseph  River.
This is the first collection of the species  from
this drainage.
                                                   54

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                                                                                  Huron-Erie Lake Plain
Species  Trends: The St. Joseph  River had  the
greatest  species  richness  and highest biotic
integrity among the Maumee River drainage
subdrainages.   The series  of dams have  reduced
biological integrity in the upstream  portions of
the drainage by reducing  migration potential
and decreasing  dissolved  oxygen levels. The
lowermost  sections  of the tributaries  had the
best biological integrity among  any of the
subdrainages.   The quality of the  riparian zones
and reduced channelization  was  considered  a
significant benefit for the preservation  of
biological integrity. It should  be noted that  the
system  recovers immediately downstream  of the
hydroelectric dams  with the best station located
below the tailwaters of the lowermost  dam.

The number  of sensitive species  also paralleled
the IBI trend with reductions in sensitive
species  richness above  the dams. Nineteen
species  of Cyprinidae were collected  from the
St. Joseph  River drainage.  Both species  of
Campostoma  and Luxilus were collected from
the drainage,  likewise two species of Cvprinella
were  documented   for the first time in the basin.
The minnow species  collected  from the  St.
Joseph  River drainage  are mostly insectivores
(63.1%), high pioneering tendencies  (42.1%),
and only a few (26.3%) of the species  being
sensitive to low dissolved  oxygen, toxic
chemicals,  and degraded  habitat.

Nine  sucker  species  were collected  during  the
current investigation. A large proportion  of the
species  are considered  sensitive  to thermal and
other habitat modifications.  The redhorses  are a
significant  part of the community (44.4%)
represented   by 4 species.   Other  sensitive
round-bodied   suckers include spotted sucker
Minvtrema melanops and northern  hogsucker.

The number of benthic  insectivore species
include 7 species  of darters, 2 species  of
madtoms,  and  a single  sculpin species.  The
rediscovery of the eastern  sand darter was an
important collection  from the current study.
Simon (1993)  documented   the species  historical
presence  in the St. Joseph,  St. Mary's and
Maumee Rivers. The species requires clean
sand  and fine gravel substrates  for feeding and
reproduction.  The number  of darter species
present in the St. Joseph  River was probably
typical of the Maumee  River drainage  before the
extensive  landuse modifications. The  stonecat
Noturus flavus and  brindled madtom  N. miurus
were found in the only the highest  quality
tributary and  mainstem  sites.

A saugeye  fishery has  been developed in the St.
Joseph  River perpetuated  by the stocking  of
juveniles (E. Braun,  IDNR.pers. comm.). No
saugeyes  were collected in our collections,
however,  some populations  had been collected
in the St.  Mary's River and Maumee River below
the junction of the St. Joseph  and  St. Mary's
Rivers (Pearson and Shipman  1978). Several
large individual sauger  and  a few walleye were
collected  from the Maumee  River. A large
proportion of young-of-the-year  were  either
stocked or indicated a successful  spawn in the
St. Mary's River near Decatur.

   St. Marvs* River Drainage

Species  Composition:  A total of 23 sites were
sampled  in the St. Mary's River. A total of 47
species were collected  (Table  12) and were
numerically dominated  by centrarchid,  cyprinid,
and catostomid species.

The fish community  assemblage  of the St.
Mary's River ranged  from a low of very poor
(score of 12; 6 stations) to a high of good
(score of 49; 1 station)  based on IBI
classification  criteria. The biotic integrity of the
St. Mary's River increased with increasing
drainage area. Stations  in the headwaters  of the
tributaries  were generally  degraded.  IBI scores
within the  St. Mary's drainage were skewed
towards lower water resource quality. Among
the 23 stations  4.3% (1) were classified  as
good; good-fair 8.7% (2 stations);  fair 13.0% (3
stations);  fair-poor 13.0% (3 stations);  poor
21.7% (5  stations); poor-very  poor  13.0% (3
stations);  and  very poor 26.1  (6 stations).  Fish
were collected at all sites  in the St.  Mary's River
drainage.  Visually it appeared  sites  which  had
low IBI values  were affected  by significant
nutrient input.
                                                   55

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Indiana Ecorecion
Species  collected only in the St. Mary's River
include river carpsucker  Carpiodes  carpio.
redear sunfish Lepomis microlopfaus.  nnd
walleye Stizostedion  vitreum. Semotilus
               white sucker  Catostomus
              fathead minnow Pimehales
promelas.  bluntnose  minnow  Piiqephales
notatus.  and green sunfish Lepomis cvaneilus
were  the dominant species  in the drainage.
Several threatened and endangered  species
were  documented  from the St. Mary's
subdrainage  including river redhorse
Moxostoma carinatq^i and  greater redhorse  M-
yalenciennesi.  The majority of sites  are
dominated  by  omnivorous,  pioneering,  tolerant
species.

Population Attributes: Longitudinal trends show
declining biological integrity in the headwaters
of the subdrainage.  Headwaters  are highly
          by agricultural runoff and  nutrient
input which results  in reduced  biological
diversity. It should be noted  that the St. Mary's
River drainage exhibited declining  biotic integrity
upstream of Decatur caused  from agricultural
loss of highly erosional  soils and input of
nutrients  from fertilizers.

The number  of indicator species  also paralleled
the IBI longitudinal  trends with reductions in
species  richness  of minnows,  suckers, and
darters.  Species  richness  was  the  lowest
observed among  the three subdrainages.

Only 14 species  of minnow were collected
usually representing  the most  tolerant (50%),
omnivorous  (50%), pioneering  (64.3%) species.
Only the largescale  stoneroller was a new
record for the subdrainage of  any  significance.
Both species  of £ajnp£§toma.   were collected
from the drainage.

Ten species  of Catostomidae  were found during
this investigation.  Trophic arrangement  of the
species  showed  equal  distribution  between
omnivores  and the more sensitive  round-bodied
insectivores.  The greater redhorse  and river
redhorse were found at several  stations.  These
are new records  for the drainage with the
greater  redhorse  never  being  documented  from
the drainage.

The number  of darter species  was  significantly
reduced being represented   by only 4 species.
The darter  species  observed reflected the
moderate  ranges of biological integrity (Table
12). Two species  of madtoms  were collected
including stonecat  and  tadpole  madtom.  Thus, a
total of 6 benthic insectivore species were
collected from the  St. Mary's subdrainage.  More
sensitive members  of the guild have been
extirpated  (Kirsch 1895; Gerking 1945).  Species
such  as blackside darter, logperch, greenside
darter, and eastern sand darter  had been
collected historically.
Reference  Sites

Reference  sites are localities which best
represent  the regional  framework  under study.
Reference  sites define  the  "reference condition"
or "least impacted"  condition which are used  to
define the  Maximum Species Richness  line
based  on the 95th percentile.  Subsequent
recalibration  of the  individual IBI metrics  can
concentrate   on these  sites during future
monitoring efforts. Few natural areas remain in
the Maumee  River drainage. The list of
candidate  sites are  based on superior  IBI
scores,  typical habitat  for the ecoregion,  and
professional  judgement  (Table 13).  The
reference  sites listed are those which achieved
he highest  biotic integrity based on species
composition,   trophic and reproductive  guilds,
catch per unit effort, and disease  factors.
                                                   56

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                                                                             Huron-Erie Lake Plain
Table  13. Reference1   sites  used  to determine   fish  community  biotic integrity
         for the Maumee  River drainage,  Indiana.
Maumee River
Drainage
St. Joseph River
Drainage
Maumee River: Allen County: at Anthony Road bridge, Ft. Wayne, St.
Joseph Twp., T SON R 13E S 06 long. 85° 06' 52" lat. 41" 04* 56 (site:
91-80).
Maumee River: Allen County, at SR 24 bridge,  New Haven,  Adams Twp.,
T 30N R 13E S 2/1 long. 85° 01' 11' lat. 41° 05' 04" (site: 91-127).
Maumee River: Allen County, at Bull Rapids  Road bridge, 4 mi NE New
Haven, Milan Twp. T31N R 14E S 22 long. 84°  52' 41" lat. 41° 09' 02"
(site: 91-64).
Maumee River: Allen County, at old Scipio Road  bridge, 0.5 mi W Ohio
State  line. T 31N R 15E S 10. long. 84° 48' 42" lat. 41° 09' 48"  (site:  91-
63).

Fish Creek: Steuben  County:  at 210A bridge, 1.5 mi W Hamilton,
Oteego Twp.,T 36N R 14E S 32. long. 84° 56' 07" lat. 41° 32' 06"
(site: 91-22).
St. Joseph  River: Allen County: at SR 24/30/14,  adjacent to Johnny
Appleseed  Park, Ft. Wayne, Wayne Twp., T 3IN R 12E S 25 long. 85° 07'
02" lat. 41° 06'45" (site: 91-139).
Fish Creek: Steuben  County:  at CR 850E bridge, 4.5 mi NE Hamilton,
Richland Twp., T 36N R 15E S  20. long. 84° 50' 25" lat. 41° 35' 04"
(site: 91-24).
Cedar Creek:  Dekalb County:  at CR 27 Road bridge, 2.25 mi  NW Waterloo,
Smithfield Twp.,T35N R 12E S 31. long. 85° 03'48"lat. 41° 26' 51"
(site: 91-100).
St. Joseph  River Allen County:  at Main Road bridge, below Cedarville
Reservoir, 0.5 mi S Cedarville,  Cedar Creek Twp. T 32N R 13E S 28
long.  85° 01' 30" lat. 41° 11' 51" (site: 91-129).

St. Mary's River: Allen County: at SR 1 bridge, Fort Wayne, Wayne Twp.
T 30N R 12E S 26. long. 85° 08' 52' lat. 41° 01' 45" (site: 91-138).
SL Mary's River: Adams County: at SR 224  bridge, Decatur, Washington
and Root Twp.,  T27/28N R 14E S 34/3.  long. 84C  55' 19" lat. 40° 50'
00" (site: 91-130).
St Mary's River: Allen County: at Hoagland  Road bridge, Poe, Marion
Twp. T 29N R 13E S  29. long.  85° 05' 11" lat. 40° 56' 14" (site: 91-132).
Reference sites in this drainage represent "least impacted"  sites and  do not reflect conditions
comparable  to excellent resource  waters of the state.
St. Marys' River
Drainage
                                                57

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 Biocriteria Comparison  of the Huron-Erie
 Lake Plain

 The States  of Ohio and Michigan share the
 Huron-Erie Lake Plain ecoregion  with Indiana.
 Ohio in an attempt  to develop  the most
 stringent  biological criteria evaluated  statewide
 biological  criteria for the ecoregions  and
 sampled extensively in Michigan during the late
 1980's  in an attempt to find additional reference
 stations  (Simon, personal  observation). It has
 been well-known that the  Huron-Erie Lake Plain
 has  been severely degraded  by the  actions of
 humans  (Ohio EPA 1989). It was a primary goal
 of this study to determine  if reference  condition
 expectations  developed from the Indiana  portion
 of the ecoregion  could advance biological
 criteria  expectations  for this region.

 The U.S. Environmental Protection Agency,
 Environmental  Research Laboratory-CorvaUis
 and  Ohio EPA collaborated  on a project to
 determine  reference  conditions for the state.
 The Stream Regionalization  Project culminated
 in the development  of statewide reference
 condition expectations  for Ohio (Whittier et al.
 1987; Ohio EPA 1989). The Huron-Erie Lake
 Plain was recognized  as having limitations for
 warmwater  habitat designations  as defined  by
 Ohio Water Quality Standards  (Ohio EPA 1989).
 Ohio EPA biologists  determined mat none of the
 wading  and headwater  sites sampled   in this
 ecoregion  reflected 'least  impacted"  conditions
 relative  to reference  sites  from the remainder  of
 the State. The Huron-Erie  Lake Plain is
 distinquished  from the other ecoregions by the
 wide-scale macrohabitat alterations  within the
 headwaters  and  small rivers. Intensive rowcrop
 agriculture and drainage practices  (i.e. channel
 modifications  to  increase  subsurface  drainage)
 have preserved  few streams.  As a result the IBI
 reflects  this lowered  biological  integrity. In order
 to determine warmwater habitat criteria for
headwater  and mid-sized streams and rivers the
Ohio EPA examined  results  from all sites
sampled between 1979 and 1986. The criteria
was  derived from metric values which
delineated  the upper 10%  of all sites sampled.
They also reviewed  historical literature to
determine expectations  for this region.
 Ohio EPA developed  a "modified warmwater
 habitat" designation for streams  in the  Huron-
 Erie Lake Plain in order to properly  characterize
 the extensive  landscape  modifications,  yet
 protect the chemical,  physical, and  biological
 integrity of these streams.  The modified
 designation is used to protect streams  and
 rivers which function as warmwater  fish
 communities  but because   of structural  and
 functional  modifications of these communities
 caused by human  induced practices  the
 community  cannot  fully attain warmwater  status.
 The return of biological integrity to sufficient
 levels representative  of "least impacted"
 conditions  are not  expected in the  near future
 due to the physical  modification of the  stream
 channel and substrate. Recovery of such areas
 to "least impacted"  conditions  is not possible
 without some recovery of the  stream channel
 and an increase  in gradient to a pre-modified
 condition  or extensive  landuse  changes  within
 the drainage.

 Two possibilities exist for explaining the
 structural  and  functional  attributes  of the Huron-
 Erie Lake Plain. First, the altered community
 attributes  are a direct result of macrohabitat
 degradation.  These  communities are
 characterized  by a predominance  of tolerant
 species,  generalists  and omnivores,  and only
 moderatley  reduced biological diversity.
 Abundance  and biomass can increase  as  a
 result  of nutrient enrichment which  cascades
 into increased  productivity  for those tolerant,
 generalise  or omnivorous species  able to endure
 the impact.

 A second  compounding  reason for reduced
 structural  and functional complexity  in the
 Huron-Erie Lake Plain community may  be a
 function of zoogeography.   The reduction  of
 available dispersal  routes post-glaciation
 required many species  to reinvade  the  area only
after the glaciers receeded  some  10,000 years
ago (Underbill 1986). Species recolonization  of
the Lake Plain was  aided by the glacial
connection   between the Wabash and Maumee
Rivers by the  Little Wabash River and by  the
Grand River connection  across  Michigan. As the
Saginaw  lobe  retreated across  Michigan a large
                                                  58

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                                                                                Huron-Erie Lake Plain
pool of water remained which was the precursor
of Lake Erie. Species capable  of tolerating
lentic, turbid, cold water were able to reinvade
the system first. Many species were unable  to
disperse  into the Great Lakes, thus, the Great
Lakes as a whole are biologically limited in
comparison  to riverine systems such  as the
Mississippi and Ohio River systems. A study by
Smith et al. (1981) in the Raisin River system
demonstrates  this effect with the headwaters  of
the system reflecting more structural  and
functional attributes  of the Eastern Com Belt
Plain fish community,  while lower sections of
the river were dominated  by  the most tolerant
species.  The lack of riffles and other
macrohabitat  features  would  not have been
present  even during pre-Columbian "pristine*
conditions. The predominance of wetlands,  low-
gradient,  seiche directed  streams and rivers of
this region would have precluded die majority of
sensitive species.  Thus, reference  conditions
need to reflect not only high  gradient  "least
impacted"  streams  but also the typical low-
gradient types of habitats  which occurred  along
the Great Lakes.

In order to compare the criteria and direction of
the individual metrics  to determine if Ohio  EPA
biological criteria could  benefit from the effort
conducted in Indiana  it is necessary   to compare
the associated  variance of the reference
conditions.  The State  of Ohio developed
biological  criteria from the analysis of over  300
reference sites statewide.  After careful analysis
of the results, the Huron-Erie Lake Plain was
separately scored  using a 90th percentile
objective.  In order to  compare  metric
expectations,  datasets  from  each  of the  12
metrics  was compared from Ohio EPA (1989) to
the current metric criteria. The Indiana dataset  is
based  on a  95th percentile of the reference
condition. Results  are summarized  in Table 14
between  Ohio Statewide  criteria and  Indiana
Huron-Erie Lake Plain expectations.

 In a comparison  (student  t-test, alpha =  0.05)
between  the two reference conditions equal
amounts  of similarities and differences exist.
 Metrics which did not differ statistically between
 Ohio Statewide criteria and Indiana Huron-Erie
Lake Plain expectations  include total number  of
species  for headwater  sites,  number of sunfish
species,  number  of minnow  species, number  of
sucker species, proportion of tolerant species  at
wadable  sites, proportion of carnivores,
proportion  of simple lithophils for wadable  sites,
and proportion of disease,  eroded  fins, lesions,
and tumors.

Metrics which exhibited  a statistical difference
between  Ohio statewide  criteria and Indiana
Huron-Erie Lake  Plain expectations  included
total number of species  at wadable sites,
number of darter species,  proportion  of tolerant
species  at headwater   sites, number of sensitive
species,  proportion of omnivores,  proportion  of
insectivores,  proportion  of pioneer  species,  and
proportion of simple lithophils at headwater
sites.  Of the  eight metrics  which have  significant
differences, 6 (75%) of the metrics  were more
stringent when using Ohio EPA's statewide
criteria. Only the proportion  of omnivores and
insectivores  were more  stringent using  Indiana's
Huron-Erie Lake  Plain criteria. The number  of
species  metric showed differences  in wadable
sites.  This may have been a function of more
speciose  areas such  as the  Eastern Corn Belt
Plain or Interior Plateau  skewing results  because
of the increased  species  richness  of these
areas.  This would have  been  exaggerated  at
larger drainage areas  where more habitat
complexity would have  been  exhibited. The
number of darter species and number of
sensitive species   showed  similar trends  with
Ohio statewide criteria exhibiting greater
biodiversity than  the Lake Plain. The proportion
of tolerant species, pioneer  species, and simple
lithophils (headwater  sites) all reflected more
stringent expectations  using  Ohio statewide
criteria than  the  ecoregion specific criteria  from
Indiana. This was anticipated since these
characteristics  are exhibited in the structural
and functional attributes of die community.  The
specific expectations   would have  to be modified
either through  lowered expectations or by
adjusting die warmwater habitat attainment
criteria as developed   by Ohio EPA.
                                                   59

-------
Indiana
Table 14. Comparison   of Ohio EPA (1989) reference  conditions  derived  from
         the Stream Regionalization  Project with reference  conditions
           developed  from Indiana portions of the Huron-Erie Lake Plain.

Metric
1. Total Number of Species
2. Number of darter species
3. Number of Sunfish species
4. Number of Minnow species
Number of Sucker species
5. Number of Sensitive species
6. Proportion Tolerant species
7. Proportion of Omnivores
8. Proportion of Insectivores
9. Proportion Pioneer species
Proportion of Carnivores
10. Catch per unit of effort
11. % Simple Lithophils
12. Proportion of DELT

Reference
Ohio EPA (1989) Statewide
Headwater'" Wadable Stream0*
21 34
7
6
10
6
9
80%
43%
74%
30%
~
~
11%
<0.1
7-8
6
-
-
-
60%
44%
80%
-
5%
-
54%
<0.1
Condi tjiflflfi

Indiana Huron-Erie Lake Plain
Headwater Wadable Stream
18 19
3
5
9
7
4
50%
25%
50%
50%
—
300
45%
<0.1
4
5
—

6
50%
25%
50%
—
5%
400
45%
<0.1
M Maximum value from 95th percentile of Maximum Species  Richness  lines at 20 mi2;
w Maximum value from 95th percentile of Maximum Species  Richness  lines at 100 mi2.
                                          60

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                                                                                Huron-Erie Lake Plain
Only two metrics were collected  differently
prohibiting further comparison.  The catch  per
unit of effort was expressed  as the number of
fish per 300 m. Samples  from this collection
included 15x the stream  width with a minimum
of SO m sampled  and the longest distance
sampled was 500 m. The number of sensitive
species  at wadable sites also  deviated in
collection and categorizing  strategy. Ohio EPA
uses the sensitive species designation  only at
headwater sites.  At larger wadable  sites, Ohio
uses an intolerant species metric which reduces
the number  of recognized sensitive  species
from the headwater  category.  This makes  the
criteria  inherently more stringent  and prohibits
comparison  of reference  conditions  between the
two datasets.

The result of this comparison  suggests  that the
Huron-Erie Lake  Plain criteria developed  during
mis study can be either directly comparable  to
statewide biological criteria developed  for the
Ohio State  Water Quality Standards  or more
stringent in  the protection of surface waters for
many metrics. It must be mentioned that
differences  in regional framework approaches
may be the  difference between these two State
strategies. In only 37.5% of the metrics did the
existing Ohio statewide criteria provide more
stringent expectations  than  what was observed
from the Indiana  portion of the Huron-Erie Lake
Plain. This shows  that "least impacted"
conditions can be estimated for the Huron-Erie
Lake Plain based on reference  conditions
developed across  political boundaries. Further
evaluation of the Ohio Huron-Erie Lake Plain
dataset  is necessary  to evaluate  the exact
deviations from the Indiana  expectations.
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                                                 63

-------
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-------
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                                                 67

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Indiana  Ecoreeion
    APPENDICES

       A.  Tolerance, trophic, and reproductive guild classifications for computing the Index of Biotic
           Integrity for Indiana taxa.

       B.  Metric specific Index of Biotic Integrity scores for sites in the Maumee River drainage.

       C.  Fish nomenclature changes for the species of fish occurring within the political boundaries of
           Indiana.
                                                 68

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APPENDIX  A.  Tolerance, trophic, and reproductive guild classifications for computing the Index of
               Biotic Integrity for Indiana taxa.                         -f—c u«, UHWX 01
                                                                                                                             Pagel
VOUCHERCD
1.00
2.00
3.00
4.00
5.00
6.00
7.00
8.00
9.00
10.00
11.00
12.00
13.00
14.00
15.00
16.00
17.00
18.00
19.00
20.00
21.00
22.00
23.00
24.00
25.00
26.00
27.00
28.00
29.00
30.00
31.00
32.00
33.00
34.00
35.00
36.00
37.00
38.00
39.00
40.00
41.00
42.00
43.00
44.00
46.00
47.00
48.00
49.00
GENUS
Lampetra
Lampetra
Petromyzon
Ichthyomyzon
Ichthyomyzon
Ichthyomyzon
Ichthyomyzon
Acipenser
Scaphirhynchus
Polyodon
Lepisosteus
Lepisosteus
Lepisosteus
Atractosteus
Amia
Anguilla
Alosa
Alosa
Dorosoma
Alosa
Dorosoma
Hiodon
Hbdon
Coregonus
Coregonus
Coregonus
Coregonus
Coregonus
Coregonus
Oncorhynchus
Oncorhynchus
Oncorhynchus
Salmo
Salmo
Salvelinus
Salvelinus
Dsmerus
Esox
Esox
Esox
Esox
Jmbra
^yprinus
iarassius
Hybognathus
tybognathus
tybognathus
Notemigonus
SPECIES
aepyptera
appendix
marinus
bdellium
castaneus
fossor
unicuspis
fulvescens
platorynchus
spathula
osseus
oculatus
platostomus
spatula
calva
rostrata
alabamae
pseudoharengus
cepedianum
chrysochloris
petenense
alosoides
tergisus
clupeaformis
artedi
hoyi
nigripinnis
reighardi
zenith icus
kisutch
tshawytscha
my kiss
salar
trutta
namaycush
fontinalis
mordax
ucius
americanus
ohioensis
masquinongy
imi
carpio
auratus
nuchalis
lankinsoni
iayi
crysoleucus
COMMON NAM
LEAST BROOK LAMPREY
AMERICAN BROOK LAMPR
SEA LAMPREY
OHIO LAMPREY
CHESTNUT LAMPREY
NORTHERN BROOK LAMP
SILVER LAMPREY
LAKE STURGEON
SHOVELNOSE STURGEON
PADDLEFISH
LONGNOSE GAR
SPOTTED GAR
SHORTNOSE GAR
ALLIGATOR GAR
BOWFIN
AMERICAN EEL
ALABAMA SHAD
ALEWIFE
GIZZARD SHAD
SKIPJACK HERRING
THREADFIN SHAD
GOLDEYE
MOONEYE
LAKE WHITEFISH
CISCO OR LAKE HERRING
BLOATER
BLACKFIN CISCO
SHORTNOSE CISCO
SHORT JAW CISCO
COHO SALMON
CHINOOK SALMON
RAINBOW TROUT
ATLANTIC SALMON
BROWN TROUT
LAKE TROUT
BROOK TROUT
RAINBOW SMELT
NORTHERN PIKE
GRASS PICKEREL
MUSKELLUNGE
GREAT LAKES MUSKELLU
CENTRAL MUDMINNOW
CARP
GOLDFISH
MISSISSIPPI SILVERY MINN
BRASSY MINNOW
CYPRESS MINNOW
GOLDEN SHINER
FEED GUILD
F
F
P
P
P
F
P
V
I
F
P
P
P
P
P
C

F
O
P
O
I
I
V
F

_
.
_
3
3
3
3
3
3
3
V
3
J
)
>
O
O
O
O
O
O

REPR GUILD
N
N
N
N
N
N
N
S
S
S
M
M
M
M
C

N
M
M
M
M
M
M
M
M
M
N
N
M
N
N
N
N
N
N
N
M
M
M
M
M
f\,
f
M
n
i

\A
M
TOLERANCE
R
R

S

S

_
_
S

_
_
_
_
T

_
_
—
.
R
R

_
.
_
_

\A
\A
M
\A
\A
VI
\A


3


•
-
•



•

-------
VOUCHERCD

53.00
54.00
55.00
56.00
57.00
58.00
59.00
60.00
61.00
62.00
63.00
64.00
65.00
66.00
67.00
68.00
69.00
70.00
71.00
72.00
73.00
74.00
75.00
76.00
77.00
78.00
79.00
80.00
81.00
82.00
83.00
84.00
85.00
86.00
87.00
88.00
89.00
90.00
91.00
92.00
93.00
94.00
95.00
96.00
97.00
98.00
99.00
100.00
101.00
GENUS

Rhinichthys
Nocomis
Nocomis
Notropis
Notropis
Notropis
Notropis
Notropis
Notropis
Notropis
Notropis
Notropis
Notropis
Notropis
Notropis
Notropis
Notropis
Notropis
Notropis
Notropis
Euricymba
hlybopsis
Hlybopsis
3henacobius
Campostoma
Dampostoma
3imephales
Dimephales
3imephales
Couesius
Dtenopharyngodon
Dhoxinus
Scardinius
typophthalmichthys
Cyprinella
Syprinella
Cyprinella
Erimystax
Erimystax
.uxilus
.uxilus
.ythrurus
.ythrurus
.ythrurus
flacrhybopsis
)psopoeodus
•xtrarius
Catostomus
Catostomus
SPECIES
atratulus
cataractae
micropogon
biguttatus
chalybaeus
hudsonius
rubellus
atherinoides
buchanani
shumardi
ludibundus
texanus
volucellus
anogenus
ariommus
blennius
boops
dorsalis
heterodon
heterolepis
photogenis
buccata
amblops
amnis
mirabilis
anomalum
oligolepis
notatus
Dromelas
vigilax
plumbeus
idella
eythrogaster
erythrophthalmus
molitrix
utrensis
spiloptera
whipplei
dissimilis
x-punctatus
chrysocephalus
cornutus
ardens
fumeus
umbratilis
storeriana
emiliae
aestivalis
catostomus
commersoni

BLACKNOSE DACE
LONGNOSE DACE
RIVER CHUB
HORNYHEAD CHUB
IRONCOLOR SHINER
SPOTTAIL SHINER
ROSYFACE SHINER
EMERALD SHINER
GHOST SHINER
SILVERBAND SHINER
SAND SHINER
WEED SHINER
MIMIC SHINER
PUGNOSE SHINER
POPEYE SHINER
RIVER SHINER
BIGEYE SHINER
BIGMOUTH SHINER
BLACKCHIN SHINER
BLACKNOSE SHINER
SILVER SHINER
SILVERJAW MINNOW
BIGEYE CHUB
PALLID SHINER
SUCKERMOUTH MINNOW
CENTRAL STONEROLLER
LARGESCALE STONEROLL
BLUNTNOSE MINNOW
FATHEAD MINNOW
BULLHEAD MINNOW
LAKE CHUB
GRASS CARP
SOUTHERN REDBELLY DA
RUDD
SILVER CARP
RED SHINER
SPOTFIN SHINER
STEELCOLOR SHINER
STREAMLINE CHUB
GRAVEL CHUB
STRIPED SHINER
COMMON SHINER
ROSEFIN SHINER
RIBBON SHINER
REDFIN SHINER
SILVER CHUB
PUGNOSE MINNOW
SPECKLED CHUB
LONGNOSE SUCKER
WHITE SUCKER
FEED GUILD
G
























H
H
O
O
D

D
H
O
D













D
REPR GUILD
s
S
N
N
M
M
S
M
M
S
M
M
M
M
S
S
S
M
M
M
S
M
S
S
S
N
N
*\
^
*\
^/
*^
*s
S
M
S
M
M
N
M
M
S
S
S
s
S
M
N
/|
M
M
*
)
*
O

T
R
I
I
I
P
I

_
I
M
R
I
s
s

I

R
S
R

I
R


_
T
T

_
T

r
T
T


R
M


/|



^
%

•
Page 2

-------
CO
'

                                        Q^Qu-Lt
   /
          I
          TJ
          ,E!
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                C 0)J3
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4

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-------
A.
                        Continued
                                                                                                                      Page 4
VOUCHERCD
152.00
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159.00
160.00
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163.00
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165.00
166.00
167.00
168.00
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170.00
171.00
172.00
173.00
174.00
175.00
176.00
177.00
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193.00
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199.00
200.00
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GENUS
Myoxocephalus
Morone
Morone
Morone
Ambloplites
Centrarchus
Lepomis
Lepomis
Lepomis
Lepomis
Lepomis
Lepomis
Lepomis
Lepomis
Lepomis
Micropterus
Micropterus
Micropterus
Pomoxis
Pomoxis
Etheostoma
Etheostoma
Etheostoma
Etheostoma
Etheostoma
Etheostoma
Etheostoma
Etheostoma
Etheostoma
Etheostoma
Etheostoma
Etheostoma
Etheostoma
Etheostoma
Etheostoma
Etheostoma
Etheostoma
Perca
Percina
Percina
Percina
Percina
Percina
Percina
Percina
Percina
Stizostedion
Stizostedion
Percina
Ammocrypta
SPECIES
thompsoni
saxatilis
chrysops
mississippiensis
rupestris
macropterus
cyanellus
gulosus
macrochirus
gibbosus
humilis
megalotis
microlophus
punctatus
symmetricus
dolomieu
salmoides
punctulatus
annularis
nigromaculatus
chlorosomum
gracile
spectabile
nigrum
asprigene
blennioides
caeruleum
camurum
exile
flabellare
histrio
maculatum
microperca
squamiceps
tippecanoe
variatum
zonale
flavescens
caprodes
sciera
evides
maculata
phoxocephala
shumardi
uranidea
vigil
vitreum
canadense
copelandi
clara
COMMON NAM
DEEPWATER SCULPIN
STRIPED BASS
WHITE BASS
YELLOW BASS
ROCK BASS
FLIER
GREEN SUNFISH
WARMOUTH
BLUEGILL
PUMPKINSEED
ORANGESPOTTED SUNFIS
LONGEAR SUNFISH
REDEAR SUNFISH
SPOTTED SUNFISH
BANTAM SUNFISH
SMALLMOUTH BASS
LARGEMOUTH BASS
SPOTTED BASS
WHITE CRAPPIE
BLACK CRAPPIE
BLUNTNOSE DARTER
SLOUGH DARTER
ORANGETHROAT DARTER
JOHNNY DARTER
MUD DARTER
GREENSIDE DARTER
RAINBOW DARTER
BLUEBREAST DARTER
IOWA DARTER
FANTAIL DARTER
HARLEQUIN DARTER
SPOTTED DARTER
LEAST DARTER
SPOTTAIL DARTER
TIPPECANOE DARTER
VARIEGATE DARTER
BANDED DARTER
YELLOW PERCH
LOGPERCH
DUSKY DARTER
GILT DARTER
BLACKSIDE DARTER
SLENDERHEAD DARTER
RIVER DARTER
STARGAZING DARTER
SADDLEBACK DARTER
WALLEYE
SAUGER
CHANNEL DARTER
WESTERN SAND DARTER
FEED GUILD
-
P
P
P
C


C







C
C
C
-
-

















-








P
P


REPR GUILD
C
M
M
M
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
M
N
S
C
M
M
S
S
M
C
M
S
N
C
S
S
M
M
S
S
S
S
S
S
S
S
S
S
S
S
TOLERANCE
-
-
-
-
M
-
T
-
P
P
-
M
-
-
-
M
-
-
-
-
-
-
-
-
-
M
M
R
-
-
S
R
-
-
R
R
M
-
M
M
R
-
I
-
S
M
-
-
S
R

-------
APPENDIX A.
Continued
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209.00
210.00
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212.00
213.00
214.00
215.00
216.00
217.00
218.00
219.00
220.00
GENUS
Ammocrypta
Crystallana
Aplodinotus
Elassoma
Notropis
Esox
Morone
Stizostedion
Lepomis
Gymnocephalus
Mylopharyngodon
Hypophthalmichthys
Neogobius
Proterorhinus
Morone
Moxostoma
Menidia
Gasterosteus
SPECIES
peilucida
asprella
grunniens
zonatum
wickliffi
lucius x maspuinongy
chrysops x saxatilis
canadense x vitreum
x-hybrid
cemuus
piceus
noblis
malanostomus
marmoratus
americana
m. breviceps
beryllina
aculeatus
COMMON NAM
EASTERNSAND DARTER
CRYSTAL DARTER
FRESHWATER DRUM
BANDED PYGMY SUNFISH
CHANNEL SHINER
TIGER MUSKIE
WIPER
SAUGEYE
SUNFISH HYBRID
RUFFE
BLACK CARP
BIGHEAD CARP
ROUND GOBY
TUBE NOSE GOBY
WHITE PERCH
OHIO REDHORSE
INLAND SILVERSIDE
THREESPINE STICKLEBAC
FEED GUILD
I
I
.
I
I
P
P
P

C
0
H
I

P
I
I
I
REPR GUILD
S
S
M
C
M
M
M
S

S
M
M
C

M
S
M
C
TOLERANCE
R
S
P

I

_
M

—
T
T
M

—
M
P

                                                                                                                                          Page5
 Feeding Guild: C - carnivore; F - filter feeder, G = generally feeder; H - herbivore; I =
 insectivore; O = omnivore; P = piscivore; Pa = Parasite; V = invertivore; - - feeding guild
 behaviorally plastic.                                                         B s
Tolerance/Sensitivity: I = common intolerant; M = moderately intolerant; P - moderately tolerant-

          1          = *** ***"'" T =             ' ~ =

-------
       APPENDIX B.  Metric specific Index of Biotic Integrity scores for sites in the Maumee River drainage.


                                         FISH COMMUNITY IBI DEVELOPMENT DATA
                                           HURON-DUE LAKX PLAUT BCORKJIOH

                                      TPS SAMPLE * :TS91011    IDEM 8:91011.0

WILLOW CRXKK              COUNTY:ALIOW        LOCATION: LIMA ID. BKHX2B                  COLLECTION DATE: 17-Jun-1991
1300hr  LATITUDE/LONGITUDE:41-  14" 55.0'/85'  10" 47.0°      DRAINAGE: 19.Qua.  miles
MT: IB -CEDAR CREEK                                      NATURAL REGION CODB:SC-BLUFFTON TILL PLAIN SECTION
«AP: HUNTBRTOWN                  HYDROLOGIC UNIT:4100003-ST.  JOSEPH RIVER BASIN (MAUMEE RIVER BASIN)

                         GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE:100meter3
                         VOLTS-30£  DC  CAPTURE DEPTH:* O.SOmetera DISTANCE  TO SHORE:6.Smetera

                                     TOTAL NUMBER OF INDIVIDUALS COUNTED   149
                                            TOTAL NUMBER OF TAXA PRESENT    10
                                           NUMDBR OF LARGE RIVER SPECIES     0
                                                NUMBER OF DARTER SPECIES     1
                                                NUMBER OF MADTOM SPECIES     0
                                               NUMBER OF SCULPIN SPECIES     0
                                 NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     1
                                               NUMBER OF SUNFISH SPECIES     2.
                                             NUMBER OF HEADWATER SPECIES     0
                                                NUMBER OP MINNOW SPECIES     4
                                     NUMBER OF ROUND BODY SUCKER SPECIES     0
                                         NUMBER OF SUCKER FAMILY SPECIES     1
                                             NUMBER OF SENSITIVE SPECIES     0
                                              NUMBER OF TOLERANT SPECIES     8
            PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
              PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                  PROPORTION OF TOLERANTS TO TOTAL COUNT    97.3%
                                  PROPORTION OF OMNIVORBS TO TOTAL COUNT    30.9%
                               PROPORTION OF INSECTIVORES TO TOTAL COUNT    55.7%
                                 PROPORTION OF CARNIVORES TO TOTAL COUNT     0.0%
                                   PROPORTION OF PIONEERS TO TOTAL COUNT    63.1%

                          PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    20.8%
                                              PERCENT D.E.L.T  ANOMOLIES     0.0%

-------
                                         INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                   OFFICE OF WATER MANAGEMENT
                                                   BIOLOGICAL STUDIES SECTION

                                              FISH COMMUNITY IBI DEVELOPMENT DATA
                                                HURON-KRJB LJUtS PLAIN BCORKGION

                                           TPS SAMPLE »:TS91012    IDEM 0:91012.0

SITE:LITTL* CBXAK CRZXK         COUNTY:ALL*H        LOCATION:F1TCS M>. BXIDGK (D/S)            COLLECTION DATE: 17-Jun-1991
TIME:1410hr  LATITUDE/LONGITUDE :4V  15"  40.5V85'   1" 30.0"      DRAINAGE: 72. Bsq. miles
SEGMEWT: 18-CEDAR CREEK                                       NATURAL REGION CODE :5C-BLUFFTON TILL  PLAIN SECTION
TOPOMAP: GARRETT                      HYDROLOGIC UNIT:4100003-ST.  JOSEPH RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR: LONGLINE      NET MESH-. 0 . 31Bcm  SAMPLING DISTANCE : IQOmeters
                             VOLTS:300   DC  CAPTURE DEPTH.< 1.20meters  DISTANCE TO SHORE:1i.Ometers

                                          TOTAL NUMBER OF INDIVIDUALS COUNTED    7£
                                                 TOTAL NUMBER OF TAXA PRESENT    13
                                                NUMBER OF LARGE RIVER SPECIES     £
                                                     NUMBER OF DARTER SPECIES     2
                                                     NUMBER OF MADTOM SPECIES     0
                                                    NUMBER OF SCULPIN SPECIES     1
                                      NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     3_
                                                    NUMBER OF SUNFISH SPECIES     3_
                                                  NUMBER OF HEADWATER SPECIES     3_
                                                     NUMBER OF MINNOW SPECIES     4
                                          NUMBER OF ROUND BODY SUCKER SPECIES     I
                                              NUMBER OF SUCKER FAMILY SPECIES     2
                                                  NUMBER OP SENSITIVE SPECIES     2
                                                   NUMBER OF TOLERANT SPECIES     5
                 PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                   PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT    26.3V

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    39.5%
                                       PROPORTION OF OMNIVORES TO TC..^L COUNT    10.5%
                                    PROPORTION OF INSECTIVORES TO TOTAL COUNT    73.7%
                                      PROPORTION OF CARNIVORES TO TOTAL COUNT     2. j%
                                         PROPORTION OF PIONEERS TO TOTAL COUNT    39.5%

                                PROPORTION  OF  SIMPLE LITHOPHILS TO TOTAL COUNT    21.1%
                                                    PERCENT D  E L T   ANOMOLIES     0.0%

-------
                                         INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                   OFFICE OF WATER MANAGEMENT
                                                   BIOLOGICAL STUDIES SECTION

                                              FISH COMMUNITY IBI DEVELOPMENT DATA
                                                HURON-BRIE LAKE PLAIN ECOREGION

                                          TPS SAMPLE #:TS91013    IDEM  #:91013.0

SITE:GKLLKR DITCH              COUNTY:ALLKW        LOCATION:S.R. 3  BRIDGE  (0/S)                COLLECTION DATE:17-Jun-1991
TIME:1510hr  LATITUDE/LONGITUDE :4_1' 12."  Ii£°/M'  ifi"  14.0°      DRAINAGE:9^4sq.  miles
SEGMENT: 18-CEDAR CREEK                                      NATURAL REGION CODE: SC-BLUFFTON TILL PLAIN SECTION
TOPOMAP: HUNTERTOWN                  HYDROLOGIC UNIT:4100003-ST. JOSEPH RIVER BASIN (MAUMEE RIVER BASIN)

                                                 NET MESH: 0.318cm  SAMPLING DISTANCE :!50meters
                                         DC  CAPTURE DEPTH:.: 0.20meters   DISTANCE  TO SHORE :2 . Smeters

                                         TOTAL NUMBER  OF INDIVIDUALS COUNTED    2£
                                                 TOTAL  NUMBER OF TAXA PRESENT      1
                                                NUMBER  OF LARGE RIVER SPECIES      0
                                                    NUMBER OF DARTER SPECIES      0
                                                    NUMBER OF MADTOM SPECIES      0
                                                    NUMBER OF SCULPIN SPECIES      0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES      0
                                                    NUMBER OF SUNFISH SPECIES      0
                                                  NUMBER OF HEADWATER SPECIES      0_
                                                    NUMBER OF MINNOW SPECIES      ID
                                         NUMBER OF ROUND BODY SUCKER SPECIES      0
                                              NUMBER OF SUCKER FAMILY SPECIES      0
                                                  NUMBER OF SENSITIVE SPECIES      0_
                                                   NUMBER OF TOLERANT SPECIES      1
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL  COUNT      0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL  COUNT      0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL  COUNT    100.0%
                                       PROPORTION OF OMNIVORES TO TOTAL  COUNT    100.0%
                                   PROPORTION OF INSECTIVORES TO TOTAL  COUNT      0.0%
                                     PROPORTION OF CARNIVORES TO TOTAL  COUNT      0.0%
                                       PROPORTION OF PIONEERS TO TOTAL  COUNT      0.0%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL  COUNT      0.0%
                                                   PERCENT D.E.L.T.  ANOMOLIES      0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               BORON-ERIK LAKS PLAIN BCORBGIOH

                                          TPS SAMPLE #:TS91014    IDEM  #:91014.0

SITE:BBCKBTT RUN               COUNTY:ALLEN        LOCATION:AOTDRH RD.  BRIDGE (TJ/S)            COLLECTION DATE:17-Jun-19£
TIME:1600hr  LATITUDE/LONGITUDE: 41'  9" 17.0°/85'  T_"   3.0°      DRAINAGE: Li±3
-------
                                         INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                   OFFICE OF WATER MANAGEMENT
                                                   BIOLOGICAL STUDIES SECTION

                                              FISH COMMUNITY IBI DEVELOPMENT DATA
                                                HURON-ERIE LAKE PLAIN KCORBGION

                                           TPS SAMPLE tt:TS91015    IDEM #:91015.0

SITE:SPY RUN                   COUNTY: ALLEN        LOCATION:S.R.  30/33/14/24 BRIDGE (U/S)      COLLECTION DATE:17-Jun-1991
TIME:1630hr  LATITUDE/LONGITUDE:41'   T_*   5^°/SS.'   i"  44.0"      DRAINAGE: 7_.7sq. miles
SEGMENT: 19-MAUMEE RIVER  (MAIN STEM)                          NATURAL REGION CODE:5C-BLUFFTON TILL PLAIN SECTION
TOPOMAP: FORT WAYNE WEST              HTOROLOGIC UNIT:4100003-ST.  JOSEPH RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH: 0 . 318cm  SAMPLING DISTANCE: IQOmeters
                             VOLTS:100   DC  CAPTURE DEPTH:< O.SOmeters  DISTANCE TO SHORE;3.2meters

                                          TOTAL NUMBER  OF INDIVIDUALS COUNTED    38
                                                 TOTAL  NUMBER OF TAXA PRESENT     1
                                                NUMBER  OF LARGE RIVER SPECIES     0
                                                     NUMBER OF DARTER SPECIES     0
                                                     NUMBER OF MADTOM SPECIES     £
                                                    NUMBER OF SCULPIN SPECIES     0_
                                      NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     0
                                                    NUMBER OF SUNFISH SPECIES     3,
                                                  NUMBER OF HEADWATER SPECIES     0
                                                     NUMBER OF MINNOW SPECIES     3
                                          NUMBER OF ROUND BODY SUCKER SPECIES     0_
                                              NUMBER OF SUCKER FAMILY SPECIES     1
                                                  NUMBER OF SENSITIVE SPECIES     0
                                                   NUMBER OF TOLERANT SPECIES     5
                 PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     ti.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    89.5%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT    26.3%
                                    PROPORTION OF INSECTIVORES TO TOTAL COUNT    34.2%
                                      PROPORTION OF CARNIVORES TO TOTAL COUNT     2.6%
                                        PROPORTION OF PIONEERS TO TOTAL COUNT    68.4%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    IB.4%
                                                   PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HORON-KRIB LARK PLAIN BCORBGION

                                                                  IDEM #:91016.0

SITE:CLEAR LAKE CXBBK          COUNTY:STBUBKH      LOCATION:C.R. 500M BRIDGE  (D/S)             COLLECTION DATE: 18-Jun-19S
TIM£:215hr   LATITUDE/LONGITUDE:41' £3"  i^O'/Si1 SjO" 42.0°      DRAINAGE: l^Bsq.  miles
SEGMENT:20-ST. JOSEPH RIVER                                 NATURAL REGION CODE:4-NORTHERN LAKES NATURAL REGION
TOPOMAP: CT.KAR LAKE                  HYDROLOGIC UNIT:4100003-ST. JOSEPH RIVER  BASIN (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH;0.318cm  SAMPLING DISTANCE:5Ometers
                             VOLTS :3_OJ>  DC  CAPTURE DEPTH :< 0.1 Ometers  DISTANCE TO SHORE: 1. Ometers

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED     3_3
                                                TOTAL NUMBER OF TAXA PRESENT      1
                                               NUMBER OF LARGE RIVER SPECIES      <>
                                                    NUMBER OF DARTER SPECIES      0
                                                    NUMBER OF MADTOM SPECIES      0
                                                   NUMBER OF SCULPIN SPECIES      0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES      0
                                                   NUMBER OF SUNFISH SPECIES      0
                                                 NUMBER OF HEADWATER SPECIES      £
                                                    NUMBER OF MINNOW SPECIES      0
                                         NUMBER OF ROUND BODY SUCKER SPECIES      0
                                             NUMBER OF  SUCKER FAMILY SPECIES      1
                                                 NUMBER OF SENSITIVE SPECIES      0
                                                  NUMBER OF TOLERANT SPECIES      i
                PROPORTION OF  LARGE RIVER  SPECIES  INDIVIDUALS TO TOTAL  COUNT      0.0%
                   PROPORTION OF  HEADWATER  SPECIES  INDIVIDUALS TO TOTAL  COUNT      0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL  COUNT    100.0%
                                       PROPORTION OF OMNIVORES TO TOTAL  COUNT    100.0%
                                    PROPORTION OF  INSECTIVORES TO TOTAL  COUNT      0.0%
                                      PROPORTION  OF CARNIVORES TO TOTAL  COUNT      0.0%
                                        PROPORTION  OF  PIONEERS TO TOTAL  COUNT      0.0%

                               PROPORTION OF SIMPLE LITHOPHILS  TO  TOTAL  COUNT    100.0%
                                                   PERCENT D.E.L.T.  ANOMOLIES      0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-ERIK LAKE PLAIN RCORBGIOM

                                          TPS SAMPLE #:TS91019

SITE:PISH CREEK                COUNTY:STKOBBN      LOCATION:C.R. 200 S Bridge  (u/a)             COLLECTION DATE: 18-Jun-1991
TIME:1105hr  LATITUDE/LONGITUDE: 4V 3J7" 33.0°/84' 49" 39.0°      DRAINAGE:29.6so. miles
SEGMENT:20-ST. JOSEPH RIVER                                 NATURAL REGION COPE:4-NORTHERN LAKES NATURAL REGION
POPOMAP:  CLEAR LAKE                  HYDROLOGIC UNIT:4100003-ST. JOSEPH RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE:IQOmeters
                             VOLTS:300  DC  CAPTURE DEPTH:< 1.0Procters  DISTANCE  TO SHORE;5.Smeters

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    98_
                                                TOTAL NUMBER OF TAXA PRESENT    12
                                               NUMBER OF LARGE RIVER SPECIES      0
                                                    NUMBER OF DARTER SPECIES      1
                                                    NUMBER OF MADTOM SPECIES      0
                                                   NUMBER OF SCULPIN SPECIES      1
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES      2
                                                   NUMBER OF SUNFISH SPECIES      4
                                                  NUMBER OF  HEADWATER SPECIES      1
                                                    NUMBER  OF MINNOW SPECIES      3
                                         NUMBER OF ROUND BODY SUCKER SPECIES      1
                                             NUMBER OF  SUCKER FAMILY SPECIES      2
                                                  NUMBER OF  SENSITIVE SPECIES      2
                                                  NUMBER OF TOLERANT SPECIES      5
                PROPORTION OF  LARGE RIVER  SPECIES INDIVIDUALS TO  TOTAL COUNT      0.0%
                   PROPORTION OF HEADWATER  SPECIES INDIVIDUALS TO  TOTAL COUNT      1.0%

                                       PROPORTION  OF TOLERANTS TO  TOTAL COUNT     86.7%
                                       PROPORTION  OF OMNIVORES TO  TOTAL COUNT     19.4%
                                    PROPORTION OF  INSECTIVORES TO  TOTAL COUNT     67.3%
                                      PROPORTION OF CARNIVORES TO  TOTAL COUNT      1.0%
                                        PROPORTION OF  PIONEERS TO  TOTAL COUNT     68.4%

                               PROPORTION OF SIMPLE LITHOPHILS TO  TOTAL COUNT     21.4%
                                                   PERCENT D.E.L.T.  ANOMOLIES      0.0%

-------
                                         INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-BRIK LAKE PLAIN BCOREQIOH

                                          TPS SAMPLE #:TS91020    IDEM #:91020.0

SITE:11H-KMfXD TRIE. H. BRANCH  COUNTY:STHOBKN      LOCATION:CR 200 S Bridg*  (d/«)              COLLECTION DATE:lB-Jun-19'
TIME;1200hr  LATITUDE/LONGITUDE:^1 3_6"  S6.00/B4' 52"   1.0°      DRAINAGE :1^2sq. miles
SEGMENT-.20-ST. JOSEPH RIVER                                 NATURAL REGION CODE:4-NORTHERN LAKES NATURAL REGION
TOPOMAP: BOON                        HYDROLOGIC UNIT;4100003-ST. JOSEPH RIVER  BASIN (MAOMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH: 0 . 318cm  SAMPLING DISTANCE: B^meters
                             VOLTS :3_0_0   DC  CAPTURE DEPTH:< 0.30meter3  DISTANCE TO SHORE : 1. 5meters

                                          TOTAL NUMBER OF INDIVIDUALS COUNTED   187
                                                TOTAL NUMBER OF TAXA PRESENT     12
                                               NUMBER OF LARGE RIVER SPECIES     0_
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     1
                                                   NUMBER OF SUNFISH SPECIES     3_
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     5
                                          NUMBER OF ROUND BODY SUCKER SPECIES     0
                                             NUMBER OF  SUCKER FAMILY SPECIES     1
                                                 NUMBER OF  SENSITIVE SPECIES     0
                                                  NUMBER OF TOLERANT SPECIES     7
                 PROPORTION OF  LARGE RIVER SPECIES  INDIVIDUALS TO TOTAL  COUNT    0.0%
                   PROPORTION OF  HEADWATER SPECIES  INDIVIDUALS TO TOTAL  COUNT    0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL  COUNT    84.0%
                                       PROPORTION OF OMNIVORES TO TOTAL  COUNT    34.8%
                                    PROPORTION OF  INSECTIVORES TO TOTAL  COUNT    47.6%
                                      PROPORTION OF CARNIVORES TO TOTAL  COUNT     0.0%
                                        PROPORTION  OF  PIONEERS TO TOTAL  COUNT    63.1%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL  COUNT     3.7%
                                                   PERCENT D.E.L.T.  ANOMOLIES     0.0%

-------
                                         INDIANA DEPARTMENT OF  ENVIRONMENTAL MANAGEMENT
                                                   OFFICE  OF WATER MANAGEMENT
                                                   BIOLOGICAL STUDIES  SECTION

                                              FISH COMMUNITY IBI  DEVELOPMENT DATA
                                                BORON-KRIS LAKE PLAIN  ECOREOION

                                                                   IDEM #:91022.0

SITE: FISH CRKBK                COUNTY: STHOBEN      LOCATION: BALL LAKB LAIR BRIDGB (D/S)        COLLECTION DATE: 18-Jun-1991
TIME:14S2hr  LATITUDE/LONGITUDE:^!.1 32"  10.0°/B4'  £6"  12.0°      DRAINAGE: 11. 6sq.  miles
SEGMENT:20-ST. JOSEPH RIVER                                  NATURAL REGION CODE:4-NORTHERN LAKES NATURAL REGION
TOPOMAP: HAMILTON                    HYDROLOGIC UNIT:4100003-ST.  JOSEPH RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE       NET MESH: 0.318cm  SAMPLING DISTANCE: IQOmeters
                             VOLTS:3_00   DC   CAPTURE DEPTH:< 0.60meters  DISTANCE TO SHORE:3 .Smeters

                                         TOTAL  NUMBER  OF  INDIVIDUALS  COUNTED    118
                                                 TOTAL  NUMBER OF  TAXA  PRESENT     15
                                                NUMBER  OF  LARGE RIVER  SPECIES      .0
                                                    NUMBER OF  DARTER  SPECIES      3_
                                                    NUMBER OF  MADTOM  SPECIES      0
                                                    NUMBER OF SCULPIN  SPECIES      0
                                     NUMBER  OF  DARTER/MADTOM/SCULPIN  SPECIES      3
                                                    NUMBER OF SUNFISH  SPECIES      5
                                                  NUMBER OF HEADWATER  SPECIES      0
                                                    NUMBER OF  MINNOW  SPECIES      2
                                         NUMBER OF ROUND  BODY  SUCKER  SPECIES      1
                                              NUMBER OF SUCKER  FAMILY  SPECIES      1
                                                  NUMBER OF SENSITIVE  SPECIES      5
                                                   NUMBER  OF TOLERANT  SPECIES      4
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS  TO TOTAL COUNT      0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS  TO TOTAL COUNT      0.0%

                                      PROPORTION OF TOLERANTS  TO TOTAL COUNT     12.7%
                                      PROPORTION OF OMNIVORES  TO TOTAL COUNT      1.7%
                                   PROPORTION OF INSECTIVORES  TO TOTAL COUNT     45.8%
                                     PROPORTION OF CARNIVORES  TO TOTAL COUNT     49.2%
                                       PROPORTION OF PIONEERS  TO TOTAL COUNT     16.9%

                              PROPORTION OF  SIMPLE LITHOPHILS  TO TOTAL COUNT      1.7%
                                                   PERCENT D.E.L.T. ANOMOLIES      0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-KRIK LAKE PLAIN BCOXBGIOH

                                          TPS  SAMPLE »:TS91023

SITE:BLAOC CRKSJC               COUNTY:STBDBKN      LOCATION:C.R. 550K BRIDGE  (U/S)             COLLECTION DATE: 18-Jun-19!
TIME:1538hr  LATITUDE/LONGITUDE:41'  33" 22^0°/84' 53"   2.0°      DRAINAGE: 8^3 sq. miles
SEGMENT:20-ST. JOSEPH RIVER                                 NATURAL REGION CODE:4-NORTHERN LAKES NATURAL REGION
TOPOMAP: HAMILTON                    HYDROLOGIC UNIT:4100003-ST. JOSEPH RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE :50meters
                             VOLTS -.300.  DC   CAPTURE DEPTH :< 0.2 Ometers  DISTANCE TO  SHORE: 2 . Ometera

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    141
                                                TOTAL NUMBER OF TAXA PRESENT     9_
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     0
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     0
                                                   NUMBER OF SUNFISH SPECIES     1
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     5
                                         NUMBER OF ROUND BODY SUCKER SPECIES     <>
                                             NUMBER OF  SUCKER FAMILY SPECIES     I
                                                 NUMBER OF SENSITIVE SPECIES     0
                                                  NUMBER  OF TOLERANT SPECIES     6
                 PROPORTION OF  LARGE RIVER  SPECIES  INDIVIDUALS TO TOTAL  COUNT     0.0%
                   PROPORTION OF  HEADWATER  SPECIES  INDIVIDUALS TO TOTAL  COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL  COUNT     90.1%
                                       PROPORTION OF OMNIVORES TO TOTAL  COUNT     71.6%
                                    PROPORTION OF  INSECTIVORES TO TOTAL  COUNT     2.8%
                                      PROPORTION OF CARNIVORES TO TOTAL  COUNT     1.4%
                                        PROPORTION  OF  PIONEERS TO TOTAL  COUNT     89.4%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL  COUNT     6.4%
                                                   PERCENT D.E.L.T.  ANOMOLIES      0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                              FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HORON-KRIK LAKE PLAIN BCORKGION

                                          TPS SAMPLE  #:TS91024    IDEM #:91024.0

SITE:FISH CREEK                COUNTY:STXDBKK      LOCATION:850B BRIDGB  (U/S)                  COLLECTION DATE:18-Jun-1991
TIME:1610hr  LATITUDE/LONGITUDE :4_1'  35"  T^'/ii1 50"  41.0°      DRAINAGE:37.Sag,  miles
SEGMENT:20-ST. JOSEPH RIVER                                 NATURAL REGION CODE:4-NORTHERN LAKES NATURAL REGION
TOPOMAP: EDON                        HYDROLOGIC UNIT:4100003-ST. JOSEPH  RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE:IQOmeters
                             VOLTS :3_0p_  DC   CAPTURE DEPTH:< 0.60meters   DISTANCE TO SHORE: 7 . Smetera

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    71
                                                TOTAL NUMBER OF TAXA PRESENT    14
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     3.
                                                    NUMBER OF MADTOM SPECIES     0
                                                    NUMBER OF SCULPIN SPECIES     1
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     4
                                                    NUMBER OF SUNFISH SPECIES     2
                                                 NUMBER OF HEADWATER SPECIES     2
                                                    NUMBER OF MINNOW SPECIES     6
                                         NUMBER OF  ROUND BODY SUCKER SPECIES     1
                                              NUMBER OF SUCKER FAMILY SPECIES     2
                                                 NUMBER OF SENSITIVE SPECIES     5
                                                  NUMBER OF TOLERANT SPECIES     4
                PROPORTION OF  LARGE RIVER SPECIES  INDIVIDUALS TO TOTAL COUNT     0.0%
                  PROPORTION OF  HEADWATER SPECIES  INDIVIDUALS TO TOTAL COUNT     11.3%

                                      PROPORTION OF TOLERANTS TO TOTAL COUNT     23.6%
                                      PROPORTION OF OMNIVORES TO TOTAL COUNT     11.3%
                                    PROPORTION OF  INSECTIVORES TO TOTAL COUNT     78.9%
                                      PROPORTION OF  CARNIVORES TO TOTAL COUNT      1.4%
                                        PROPORTION  OF  PIONEERS TO TOTAL COUNT     28.2%

                               PROPORTION OF SIMPLE  LITHOPHILS TO TOTAL COUNT     53.5%
                                                   PERCENT D.E.L.T.  ANOMOLIES      0.0%

-------
                                         INDIANA DEPARTMENT OF  ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES  SECTION

                                              FISH COMMUNITY IBI  DEVELOPMENT DATA
                                               HURON- BRIE LAKE PLAIN  BCOKEGICN

                                           TPS SAMPLE »:TS91042    IDEM It: 91042.0

SITE:WUm DITCH                COUNTY:ALLES        LOCATION:SCIPIO RD.  BRHX3X (U/S)            COLLECTION DATE:24-Jun-199
TIME:1400hr  LATITUDE/LONG I TUDE:4_1'  12."  33.0°/84' 49i"  34.0°       DRAINAGE: 5.3 sq. miles
SEGMENT; 19-MAUMBE RIVER  (MAIN STEM)                         NATURAL REGION CODE:6-BLACK SWAMP NATURAL REGION
TOPOMAP: WOODBURN NORTH               HYDROLOGIC UNIT:4100005-MAUMEE RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR-.LONGLINE     NET MESH: 0 . 31Bcm  SAMPLING DISTANCE :£0meters
                             VOLTS:3_00   DC  CAPTURE DEPTH:< 0.70meter3 DISTANCE TO SHORE: 2 . 2metera

                                          TOTAL NUMBER  OF  INDIVIDUALS  COUNTED    194
                                                TOTAL  NUMBER OF  TAXA  PRESENT     16_
                                               NUMBER  OF  LARGE RIVER  SPECIES      0
                                                    NUMBER  OF  DARTER  SPECIES      2
                                                    NUMBER  OF  MADTOM  SPECIES      0
                                                    NUMBER OF SCULPIN  SPECIES      £
                                      NUMBER OF DARTER/MADTOM/SCULPIN  SPECIES      2
                                                    NUMBER OF  SUNFISH  SPECIES      2_
                                                  NUMBER OF  HEADWATER  SPECIES      0
                                                    NUMBER  OF  MINNOW  SPECIES      £
                                          NUMBER  OF ROUND BODY SUCKER  SPECIES      0
                                              NUMBER OF SUCKER FAMILY  SPECIES      0
                                                  NUMBER OF  SENSITIVE  SPECIES      1
                                                   NUMBER OF TOLERANT SPECIES      §
                 PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                   PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    54.1%
                                       PROPORTION OF OMNIVORES ""O TOTAL COUNT    21.6%
                                    PROPORTION OF INSECTIVORES TO TOTAL COUNT    54.1%
                                      PROPORTION OF CARNIVORES TO TOTAL COUNT     1.0%
                                        PROPORTION OF PIONEERS TO TOTAL COUNT    62.4%

                               PROPORTION OF  SIMPLE LITHOPHILS TO TOTAL COUNT    18.6%
                                                   PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                         INDIANA DEPARTMENT OF  ENVIRONMENTAL MANAGEMENT
                                                   OFFICE OF WATER MANAGEMENT
                                                   BIOLOGICAL STUDIES SECTION

                                              FISH COMMUNITY IBI  DEVELOPMENT DATA
                                                HURON-BRIE LAKE PLAIN BCORKGION

                                           TPS SAMPLE #:TS91043    IDEM »:91043.0

SITE:BLACK CRBXX               COUNTY:ALLKN        LOCATION:SCHXFFER RD.  BRIDGE (TT/S)          COLLECTION DATE:24-Jun-1991
TIME:1430hr  LATITUDE/LONGITUDE: 4V  11°   
                                              NUMBER OF SUCKER  FAMILY SPECIES     i
                                                  NUMBER OF SENSITIVE SPECIES     0
                                                   NUMBER OF TOLERANT SPECIES     5
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS  TO TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS  TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS  TO TOTAL COUNT    74.6%
                                       PROPORTION OF OMNIVORES  TO TOTAL COUNT    58.7%
                                    PROPORTION OF INSECTIVORES  TO TOTAL COUNT    34.9%
                                      PROPORTION OF CARNIVORES  TO TOTAL COUNT     1.6%
                                        PROPORTION OF PIONEERS  TO TOTAL COUNT    76.2%

                              PROPORTION OF SIMPLE LITHOPHILS  TO TOTAL COUNT    14.3%
                                                   PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                         INDIANA DEPARTMENT  OF  ENVIRONMENTAL MANAGEMENT
                                                   OFFICE  OF WATER MANAGEMENT
                                                   BIOLOGICAL STUDIES  SECTION

                                              FISH  COMMUNITY IBI  DEVELOPMENT DATA
                                               BORON-KRIK XJLKX PLAIN  BCORBGION

                                           TPS SAMPLE #:TS91044    IDEM #;91044.0

SITE:GAR CRKSK                 COUNTY:ALLKH        LOCATION:BKRTHMID  RD.  BRIDGE (U/S)          COLLECTION DATE:24-Jun-199
TIME:1515hr  LATITUDE/LONGITUDE :41'  £"  50.5°/84'  5£"  34.0"      DRAINAGE: 10.6sq.  miles
S3GMENT:19-MAOMEE RIVER  IMAIN STEM)                          NATURAL REGION CODE:6-BLACK SWAMP NATURAL REGION
TOPOMAP: MAPLES                      HYDROLOGIC UNIT:4100005-MAHMEE RIVER BASIN (MAUMEE RIVER BASIN)

                             GEARiLONGLINE     NET MESH: 0 . 318cm  SAMPLING DISTANCE: 5j>meters
                             VOLTS-.30.0   DC  CAPTURE DEPTH :< 0.30meters DISTANCE TO SHORE : 3 . 7meters

                                          TOTAL NUMBER  OF  INDIVIDUALS  COUNTED   125
                                                TOTAL  NUMBER OF  TAXA  PRESENT    13
                                               NUMBER  OF  LARGE RIVER  SPECIES     0.
                                                    NUMBER  OF  DARTER  SPECIES     3_
                                                    NUMBER  OF  MADTOM  SPECIES     0
                                                    NUMBER OF SCULPIN  SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN  SPECIES     3.
                                                    NUMBER OF SUNFISH  SPECIES     1
                                                  NUMBER OF  HEADWATER  SPECIES     0
                                                    NUMBER  OF  MINNOW  SPECIES     6
                                          NUMBER  OF ROUND BODY  SUCKER  SPECIES     0
                                              NUMBER OF SUCKER  FAMILY  SPECIES     1
                                                  NUMBER OF  SENSITIVE  SPECIES     0
                                                   NUMBER OF TOLERANT  SPECIES     6
                 PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS  TO TOTAL COONT     0.0%
                   PROPORTION OF HEADWATER SPECIES INDIVIDUALS  TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS  TO TOTAL COUNT    29.6%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT    11.2%
                                    PROPORTION OF INSECTIVORES TO TOTAL COUNT    27.3%
                                      PROPORTION OF CARNIVORES TO TOTAL COUNT     0.0%
                                        PROPORTION OF PIONEERS TO TOTAL COUNT    79.2%

                               PROPORTION OF  SIMPLE LITHOPHILS TO TOTAL COUNT    12.8%
                                                   PERCENT  D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                              FISH COMMUNITY IBI DEVELOPMENT DATA
                                               BORON-BRIE LAEX PLAIN BCOREGION

                                          TPS SAMPLE  #:TS91045    IDEM  #:91045.0

SITEiCJLCHEIT DITCH             COUNTY.-ALLKH        LOCATION:QREKN ROAD  BRIDOK  (0/S)            COLLECTION DATE:24-Jun-1991
riME:160Shr  LATITUDE/LONGITUDE:41.'  3." 22.0°/8S'  0" 40.5°      DRAINAGE:6,-isq. miles
SEGMENT: 19-MAUMEE RIVER  (MAIN STEM)                         NATURAL REGION CODE:5C-BLUFFTON TILL PLAIN SECTION
rOPOMAP:  FORT HAYNK EAST             HYDROLOGIC UNIT:410000S-MAUMEE RIVER  BASIN (MAUMEE RIVER BASIN)

                             GEAR: LONGLINE      NET MESH: 0.318cm  SAMPLING DISTANCE: IQOmeters
                             VOLTS:3CU)  DC   CAPTURE DEPTH:-: O.SOmetera   DISTANCE TO  SHORE;4.Ometera

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    71
                                                TOTAL NUMBER OF TAXA PRESENT     7
                                               NUMBER OF LARGE RIVER SPECIES     £
                                                    NUMBER OF DARTER SPECIES     0
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER  OF DARTER/MADTOM/SCULPIN SPECIES     0
                                                   NUMBER OF SONFISH SPECIES     1
                                                 NUMBER OF HEADWATER SPECIES     £
                                                    NUMBER OF MINNOW SPECIES     4
                                         NUMBER OF ROUND BODY SUCKER SPECIES     0
                                              NUMBER OF  SUCKER FAMILY SPECIES     1
                                                 NUMBER OF  SENSITIVE SPECIES     0
                                                  NUMBER OF TOLERANT SPECIES     7
                PROPORTION OF  LARGE RIVER  SPECIES  INDIVIDUALS TO  TOTAL  COUNT     0.0%
                  PROPORTION OF HEADWATER  SPECIES  INDIVIDUALS TO  TOTAL  COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO  TOTAL  COUNT    100.0%
                                       PROPORTION OF OMNIVORES TO  TOTAL  COUNT     56.3%
                                    PROPORTION OF  INSECTIVORES TO  TOTAL  COUNT     33.8%
                                      PROPORTION OF CARNIVORES TO  TOTAL  COUNT     0.0%
                                        PROPORTION  OF  PIONEERS TO  TOTAL  COUNT     47.9%

                               PROPORTION OF  SIMPLE LITHOPHILS TO  TOTAL  COUNT     40.8%
                                                   PERCENT D.E.L.T.  ANOMOLIES      0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HORON-miB LUCE PLAIN SCOREQION

                                          TPS SAMPLE #:TS91046    IDEM #:91046.0

SITE:TRIER DITCH               COUNTY:ALLKK        LOCATION:ADAMS CXHTKR RD. BRIDGS  (D/S)      COLLECTION DATE:24-Jun-199
TIME:1645hr  LATITUDE/LONGITUDE:41'  3"  3^°/8S.'  3" 30.0°      DRAINAGE:SJ)sq. miles
SEGMENT .-19-MAOMEE RIVER  (MAIN STEM)                         NATURAL REGION CODE: 5C-BLOFFTON TILL PLAIN SECTION
TOPOMAP:  PORT WAYNE EAST             HYDROLOGIC UNIT:4100005-MAUMBE RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE:BOmeters
                             VOLTS: 300  DC  CAPTURE DEPTH:* 0.40meters  DISTANCE TO  SHORE: 3 . Ometers

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   190
                                                TOTAL NUMBER OF TAXA PRESENT    10
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     0
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     0
                                                   NUMBER OF SUNFISH SPECIES     2
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     6
                                         NUMBER OF ROUND BODY SUCKER SPECIES     0
                                             NUMBER OF SUCKER FAMILY SPECIES     1
                                                 NUMBER OF SENSITIVE SPECIES     0
                                                  NUMBER OF TOLERANT SPECIES     5
                PROPORTION OF LARGE  RIVER  SPECIES  INDIVIDUALS TO TOTAL COOHT     0.0%
                   PROPORTION OF HEADWATER  SPECIES  INDIVIDUALS TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT     86.3*
                                       PROPORTION OF OMNIVORES T) TOTAL COUNT     38.4%
                                    PROPORTION  OF INSECTIVORES TO TOTAL COUNT     17.9%
                                      PROPORTION OF CARNIVORES TO TOTAL COUNT     0.5%
                                        PROPORTION  OF  PIONEERS TO TOTAL COUNT     90.0%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT     4.7%
                                                   PERCENT D.E.L.T.  ANOMOLIES      0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-ERIE LAKE PLAIN ECORBOION

                                                                  IDEM #:91060.0

SITE:LITTLB CEDAR CREEK        COUNTYrNOBLE        LOCATION:1150E  (D/S)                        COLLECTION DATE:26-Sep-1991
riME:hr      LATITUDE/LONGITUDE:41' 16" 34.0°/85' 13" 40.0°      DRAINAGE:5.Osq. miles
SEGMENT: 18-CEDAR CREEK                                      NATURAL REGION CODE:4-NORTHERN LAKES NATURAL REGION
lOPOMAP: GARRETT                     HYDROLOGIC UNIT:4100003-ST. JOSEPH RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR: LONGLINE      NET MESH;0.318cm  SAMPLING DISTANCE: BOmeters
                             VOLTS :3JK>  DC  CAPTURE DEPTH:< 0.40meter3  DISTANCE TO SHORE: 6 . Ometera

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    89
                                                TOTAL NUMBER OF TAXA PRESENT     8_
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     0
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     1
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     1
                                                   NUMBER OF SUNFISH SPECIES     1
                                                  NUMBER OF  HEADWATER SPECIES     2
                                                    NUMBER  OF MINNOW SPECIES     4
                                         NUMBER  OF ROUND  BODY SUCKER SPECIES     0
                                              NUMBER OF  SUCKER FAMILY SPECIES     3.
                                                  NUMBER OF  SENSITIVE SPECIES     0
                                                  NUMBER  OF TOLERANT SPECIES     5
                PROPORTION OF  LARGE RIVER  SPECIES INDIVIDUALS TO  TOTAL COUNT     0.0%
                  PROPORTION OF  HEADWATER  SPECIES INDIVIDUALS TO  TOTAL COUNT     14.6%

                                       PROPORTION OF  TOLERANTS TO  TOTAL COUNT     78.7%
                                       PROPORTION OF  OMNIVORES TO  TOTAL COUNT     18.0%
                                    PROPORTION OF INSECTIVORES TO  TOTAL COUNT     33.7%
                                      PROPORTION OF  CARNIVORES TO  TOTAL COUNT      0.0%
                                        PROPORTION OF  PIONEERS TO  TOTAL COUNT     51.7%

                               PROPORTION OF SIMPLE  LITHOPHILS  TO  TOTAL COUNT     41.6%
                                                   PERCENT D.E.L.T.  ANOMOLIES      0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HORON-KRIB UXE PLAIN ECORBGION

                                          TPS SAMPLE #:TS91061    IDEM #:91061.0

SITE:BIACK CREEK               COUNTY:NOBLE        LOCATION:C.R. 450S  (0/S)                    COLLECTION DATE:26-Jun-195
TIME:1514hr  LATITUDE/LONGITUDE:45_' V7"  8^0°/.851 12" 30.0"      DRAINAGE: 19.Isq. miles
SEGMENT: 18-CEDAR CREEK                                      NATURAL REGION CODE:4-NORTHERN  LAKES NATURAL REGION
TOPOMAP: GARRETT                     HYDROLOGIC UNIT-.4100003-ST. JOSEPH RIVER BASIN  (MAUMEE RIVBR BASIN)

                             GEAR:LONGLINE      NET MESH: 0 . 318cm  SAMPLING DISTANCE: IQOmeters
                             VOLTS: 300  DC  CAPTURE DEPTH :< 0.20meters  DISTANCE TO  SHORE: 5 . Smeters

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    63
                                                TOTAL NUMBER OF TAXA PRESENT    10
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     0
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     0
                                                   NUMBER OF SUNFISH SPECIES     3_
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     ]5
                                         NUMBER OF ROUND BODY SUCKER SPECIES     0
                                             NUMBER OF SUCKER FAMILY SPECIES     0
                                                 NUMBER OF SENSITIVE SPECIES     0
                                                  NUMBER OF TOLERANT SPECIES     4
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    68.3%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT    27.0%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    49.2%
                                      PROPORTION OF CARNIVORES TO TOTAL COUNT     3.3%
                                        PROPORTION OF  PIONEERS TO TOTAL COUNT    81.0%

                              PROPORTION OF  SIMPLE LITHOPHILS TO TOTAL COUNT     6.3%
                                                  PERCENT  D.E.L.T.  ANOMOLIES     0.0%

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                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                              FISH COMMUNITY IBI DEVELOPMENT DATA
                                               BORON-BUB LAKE PLAIN KCOREGION

                                          TPS SAMPLE  tt:TS91062    IDEM #:91062.0

SITE:WILLOW CRKKK              COONTY:NOBLS         LOCATION:C.R. 1000N  (D/S)                   COLLECTION DATE: 26-Jun-1991
TIME:1548hr  LATITUDE/LONGITUDE: 41'  16." 32.0°/8S' 13"   9.0°      DRAINAGE -.T/Tsq. miles
SEGMENT: 18-CEDAR_CREEK                                      NATURAL REGION CODE:4-NORTHERN LAKES NATURAL REGION
TOPOMAP: GARRETT                     HYDROLOGIC UNIT:4100003-ST. JOSEPH RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH: 0. 318cm  SAMPLING DISTANCE :50.meters
                             VOLTS:30£ DC   CAPTURE DEPTH :< 0.40meter3  DISTANCE TO SHORE: 2 . Smeters

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    50
                                                TOTAL NUMBER OF TAXA PRESENT     6
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     £
                                                    NUMBER OF MADTOM SPECIES     £
                                                    NUMBER OF SCULPIN SPECIES     0
                                     NUMBER  OF DARTER/MADTOM/SCULPIN SPECIES     0
                                                    NUMBER OF SUNFISH SPECIES     2
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     1
                                         NUMBER OF  ROUND BODY SUCKER SPECIES      0
                                              NUMBER OF SUCKER FAMILY SPECIES      0
                                                 NUMBER OF SENSITIVE SPECIES      0
                                                  NUMBER OF TOLERANT SPECIES     4
                 PROPORTION OF  LARGE RIVER  SPECIES  INDIVIDUALS TO TOTAL COUNT     0.0%
                   PROPORTION OF  HEADWATER  SPECIES  INDIVIDUALS TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS  TO TOTAL COUNT    80.0%
                                       PROPORTION  OF OMNIVORES TO TOTAL COUNT     0.0%
                                    PROPORTION OF  INSECTIVORES  TO TOTAL COUNT    34.0%
                                      PROPORTION OF  CARNIVORES TO TOTAL COUNT     8.0%
                                        PROPORTION OF  PIONEERS  TO TOTAL COUNT    70.0%

                               PROPORTION OF SIMPLE  LITHOPHILS  TO TOTAL COUNT     0.0%
                                                   PERCENT D.E.L.T.  ANOMOLIES     0.0%

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                                         INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                              FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-KRIE LAKX PLAIN KCORBOION

                                                                  IDEM #:91063.0

SITE:MMJMBK RIVKR              COUNTY:ALLEN       LOCATION:OLD SCIPIO RD (D/S)                COLLECTION DATE:27-Jun-19S
TIME:1500hr  LATITUDE/LONGITUDE -.41'  2"  48.0°/84' 48>" 42.0°      DRAINAGE:•*«.*sq. miles
SEGMENT: 19-MMJMEE RIVER  (MAIN STEM)                         NATURAL REGION CODE:6-BLACK SWAMP NATURAL REGION
TOPOMAP: HOODBURN NORTH              HYDROLOGIC UNIT:4100005-MAOMEE RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:SPORT  YAK II  NET MESH:0.318cm  SAMPLING DISTANCE:SOOmeters
                             VOLTS: 300   DC   CAPTURE DEPTH:< 1. IQmetera  DISTANCE TO  SHORE:57 . Ometera

                                          TOTAL NUMBER OF INDIVIDUALS COUNTED   280
                                                TOTAL NUMBER OF TAXA PRESENT    28
                                               NUMBER OF LARGE RIVER SPECIES     4
                                                    NUMBER OF DARTER SPECIES     5
                                                    NUMBER OF MADTOM SPECIES     1
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     6
                                                   NUMBER OF SUNFISH SPECIES     3
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     8.
                                          NUMBER OF ROUND BODY SUCKER SPECIES     3.
                                              NUMBER OF  SUCKER FAMILY SPECIES     i
                                                 NUMBER OF SENSITIVE SPECIES     7
                                                  NUMBER OF TOLERANT SPECIES     11
                 PROPORTION OF LARGE RIVER SPECIES  INDIVIDUALS TO TOTAL COUNT      5.4%
                   PROPORTION OF HEADWATER SPECIES  INDIVIDUALS TO TOTAL COUNT      0.0%

                                       PROPORTION OF TOLERANTS  TO TOTAL COUNT     47.5%
                                       PROPORTION OF  OMNIVORES  TO TOTAL COUNT     12.1%
                                    PROPORTION OF INSECTIVORES  TO TOTAL COUNT     70.0%
                                      PROPORTION OF CARNIVORES TO TOTAL COUNT      4.6%
                                        PROPORTION OF PIONEERS TO TOTAL COUNT     40.0%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT     26.1%
                                                   PERCENT D.E.L.T.  ANOMOLIES      0.0%

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                                         INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                   OFFICE OF WATER MANAGEMENT
                                                   BIOLOGICAL STUDIES SECTION

                                              FISH COMMUNITY IBI DEVELOPMENT DATA
                                                HURON-ERIB LAKE PLAIN BCORBGION

                                           TPS SAMPLE (f:TS9JJ064    IDEM #:91064.0

SITE:MAUHBB RIVER               COUNTY:ALLKN        LOCATION:BULL RAPIDS RD. (D/S)              COLLECTION DATE-.27-Jun-1991
TIME:.910hr   LATITUDE/LONGITUDE :4i'   9"  26.0°/84' 52" 39.0"      DRAINAGE:«*«.*sct.  miles
SEGMENT: 19-MAOMBE RIVER  (MAIN STEM)                          NATURAL REGION CODE:6-BLACK SWAMP NATURAL REGION
TOPOMAP: GRABIL                       HYDROLOGIC UNIT:4100005-MAUMEE RIVER BASIN (MAUMEE RIVER BASIN)

                              GEAR:SPORT  YAK II  NET MESH:0 .318cm  SAMPLING DISTANCE-.SOOmeters
                              VOLTS:3JH>  DC  CAPTURE DEPTH:< l.lOmeters  DISTANCE TO SHORE : 73 . Ometers

                                          TOTAL NUMBER OF INDIVIDUALS COUNTED   340
                                                 TOTAL NUMBER OF TAXA PRESENT    24
                                                NUMBER OF LARGE RIVER SPECIES     3_
                                                     NUMBER OF DARTER SPECIES     1
                                                     NUMBER OF MADTOM SPECIES     1
                                                    NUMBER OF SCHLPIN SPECIES     0
                                      NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     2
                                                    NUMBER OF SUNFISH SPECIES     3.
                                                  NUMBER OF HEADWATER SPECIES     <>
                                                     NUMBER OF MINNOW SPECIES     9_
                                          NUMBER OF ROUND BODY SUCKER SPECIES     4
                                              NUMBER OF SUCKER FAMILY SPECIES     5
                                                  •NUMBER OF SENSITIVE SPECIES     £
                                                   NUMBER OF TOLERANT SPECIES     £
                 PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT    11.5%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    28.8%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT    12.6%
                                    PROPORTION OF INSECTIVORES TO TOTAL COUNT    80.9%
                                      PROPORTION OF CARNIVORES TO TOTAL COUNT     4.7%
                                        PROPORTION OF PIONEERS TO TOTAL COUNT    13.2%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    29.7%
                                                   PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                         INDIANA DEPARTMENT OF  ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                              FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-ERIK LAKE PLAIN BCORBGION

                                          TPS SAMPLE #:TS91065   IDEM #:91065.0

SITE:SORSKH DITCH              COUNTYrAliKH        LOCATIONiMOROJUI RD.  (T7/S)                   COLLECTION DATE: 01-Jul-19S
TIME:1310hr  LATITUDE/LONGITUDE: 41.'  £"  42.0°/84' 49" 25.0°      DRAINAGE :8_.3sq. miles
SEGMENT:21-ST. MARYS RIVER                                  NATURAL REGION CODE:6-BLACK SWAMP NATURAL REGION
TOPOMAP: HOODBORN SOUTH              HYDROLOGIC UNIT:4100007-FLATROCK CREEK BASIN  (MADMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH: 0 . 316cm  SAMPLING DISTANCE:5£meters
                             VOLTS :300_   DC  CAPTURE DEPTH:< 0 . IQmeters  DISTANCE TO  SHORE: 0 . Smeters

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED     51
                                                TOTAL NUMBER OF TAXA PRESENT     8
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF  DARTER SPECIES     0
                                                    NUMBER OF  MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     0
                                                   NUMBER OF SUNFISH SPECIES     2
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF  MINNOW SPECIES     2
                                         NUMBER OF ROUND BODY  SUCKER SPECIES     <)
                                              NUMBER OF SUCKER  FAMILY SPECIES     ()
                                                 NUMBER OF SENSITIVE SPECIES     0
                                                  NUMBER OF TOLERANT SPECIES     5
                PROPORTION OF LARGE  RIVER SPECIES INDIVIDUALS  TO TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS  TO TOTAL COUNT     0.0%

                                      PROPORTION OF TOLERANTS  TO TOTAL COUNT     66.7%
                                      PROPORTION OF OMNIVORES  TO TOTAL COUNT     7.8%
                                    PROPORTION OF INSECTIVORES  TO TOTAL COUNT     88.2%
                                     PROPORTION OF CARNIVORES  TO TOTAL COUNT     3.9%
                                        PROPORTION OF PIONEERS  TO TOTAL COUNT     7.8%

                              PROPORTION OF SIMPLE LITHOPHILS  TO TOTAL COUNT     0.0%
                                                  PERCENT  D.E.L.T. ANOMOLIES     0.0%

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                                         INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                   OFFICE  OF WATER MANAGEMENT
                                                   BIOLOGICAL STUDIES  SECTION

                                              FISH COMMUNITY IBI  DEVELOPMENT DATA
                                                HURON-ERIE LAKE PIAIH  KCORKGION

                                           TPS SAMPLE #:TS91066     IDEM #:91066.0

SITE:HOrFKMf CRBSK              COUNTY:ALLEN        LOCATION:LORTIB RD.  (U/S)                   COLLECTION DATE: 01-Jul-1991
TIME: 1345hr  LATITUDE/LONGITUDE:41.'  \*  50.0°/e4'  50." 38.0°       DRAINAGE:45.tag,  miles
SEGMENT:21-ST. MARYS RIVER                                  NATURAL REGION CODE:4-NORTHERN LAKES NATURAL REGION
TOPOMAP: WOODBURN SOUTH              HYDROLOGIC UNIT:4100007-FLATROCK CREEK BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE:IQOmeters
                             VOLTS:300   DC  CAPTURE DEPTH:< 0.70meters  DISTANCE TO SHORE:6.Smeters

                                          TOTAL NUMBER OF  INDIVIDUALS  COUNTED    190
                                                 TOTAL NUMBER OF  TAXA  PRESENT     13_
                                                NUMBER OF  LARGE RIVER  SPECIES      0
                                                     NUMBER OF DARTER  SPECIES      2.
                                                     NUMBER OF MADTOM  SPECIES      I
                                                    NUMBER OF SCULPIN  SPECIES      0
                                     NUMBER OF DARTER/MADTOM/SCULPIN  SPECIES      3_
                                                    NUMBER OF SUNFISH  SPECIES      3_
                                                  NUMBER OF HEADWATER  SPECIES      £
                                                     NUMBER OF MINNOW  SPECIES      4
                                          NUMBER OF ROUND  BODY SUCKER  SPECIES      0
                                              NUMBER OF SUCKER FAMILY  SPECIES      1
                                                  NUMBER OF SENSITIVE  SPECIES      0
                                                   NUMBER  OF TOLERANT  SPECIES      6
                 PROPORTION OF  LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0*
                   PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    72.6%
                                       PROPORTION OF OMSIVORES  TO TOTAL COUNT    27.9%
                                    PROPORTION OF INSECTIVORES  TO TOTAL COUNT    70.5%
                                      PROPORTION OF CARNIVORES  TO TOTAL COUNT     0.0%
                                        PROPORTION OF PIONEERS  TO TOTAL COUNT    55.8%

                               PROPORTION OF SIMPLE LITHOPHILS  TO TOTAL COUNT     3.7%
                                                   PERCENT D.E.L.T. ANOMOLIES     0.0%

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                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               BORON-ERIK LAKE PLAIN KCORKQION

                                          TPS SAMPLE tt:TS91067    IDEM »:91067.0

SITE:FLATRQCK CREEK            COUNTY: ALLKN        LOCATION-.HOFFHMJ RD. BRIDGE  (O/S)           COLLECTION DATE : 01 - Jul-19S
TIME:1430hr  LATITUDE/LONGITUDE:40' 59" 36.0°/84' 51° 48.0"      DRAINAGE:43.33q. miles
SEGMENT:21-ST. MARYS RIVER                                  NATURAL REGION CODE:6-BLACK  SWAMP  NATURAL REGION
TOPOMAP: DIXON                       HYDROLOGIC UNIT:4100007-FLATROCK CREEK BASIN  (MADMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE:IQOmeters
                             VOLTS: 300  DC  CAPTURE DEPTH :< 0.40meters  DISTANCE TO SHORE: 6 . Ometera

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    134
                                                TOTAL NUMBER OF TAXA PRESENT    10
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     1
                                                   NUMBER OF SONFISH SPECIES     1
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     7
                                         NUMBER  OF ROUND BODY SUCKER SPECIES     0
                                             NUMBER OF  SUCKER FAMILY SPECIES     0
                                                 NUMBER OF SENSITIVE SPECIES     1
                                                  NUMBER  OF TOLERANT SPECIES     4
                 PROPORTION OF  LARGE RIVER  SPECIES  INDIVIDUALS TO TOTAL  COUNT    0.0%
                   PROPORTION OF  HEADWATER  SPECIES  INDIVIDUALS TO TOTAL  COUNT    0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL  COUNT    80.6%
                                       PROPORTION OF OMNIVOREo TO TOTAL  COUNT    13.4%
                                    PROPORTION OF INSECTIVORES TO TOTAL  COUNT    29.1%
                                      PROPORTION OF CARNIVORES TO TOTAL  COUNT     0.0%
                                        PROPORTION  OF  PIONEERS TO TOTAL  COUNT    85.1%

                               PROPORTION OF SIMPLE LITHOPHILS  TO TOTAL  COUNT     3.7%
                                                   PERCENT D.E.L.T.  ANOMOLIES     0.0%

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                                         INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                   OFFICE OF WATER MANAGEMENT
                                                   BIOLOGICAL STUDIES SECTION

                                              FISH COWTONITY IBI  DEVELOPMENT DATA
                                                HURON-BRIE LAKE PLAIN KCORBGION

                                           TPS SAMPLE #:TS91068    IDEM (t: 91068.0

SITE:BOHNCE DITCH              COUNTY:ALLKH        LOCATION:MONROKVILLE RD.                    COLLECTION DATE: 01-Jul-1991
TIME:1517hr  LATITUDE/LONGITUDE: 40 '  51"  41.0<)/84'  53"  58.0°      DRAINAGE: 10.3 3d.  miles
SEGMENT:21-ST. MARYS RIVER                                   NATURAL REGION CODE:6-BLACK SWAMP NATURAL REGION
TOPOMAP: HOAGLAND                    HYDROLOGIC UNIT:4100007-FIATROCK CREEK BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH: 0.318cm  SAMPLING DISTANCE:5J)meters
                             VOLTS:3£0   DC  CAPTURE DEPTH :< 0 . SOmeters  DISTANCE TO SHORE: 3 . Smeters

                                         TOTAL NUMBER  OF INDIVIDUALS COUNTED   242
                                                 TOTAL  NUMBER OF  TAXA PRESENT    12
                                                NUMBER  OF LARGE RIVER SPECIES    0
                                                     NUMBER OF DARTER SPECIES    2
                                                     NUMBER OF MADTOM SPECIES    0
                                                    NUMBER OF SCULPIN SPECIES    0
                                     NUMBER OF DARTER/MADTOM/SCUWIN SPECIES    2.
                                                    NUMBER OF SUNFISH SPECIES    1
                                                  NUMBER OF HEADWATER SPECIES    £
                                                     NUMBER OF MINNOW SPECIES    7
                                         NUMBER OF ROUND BODY SUCKER SPECIES    0
                                              NUMBER OF SUCKER FAMILY SPECIES    1
                                                  NUMBER OF SENSITIVE SPECIES    0
                                                   NUMBER OF TOLERANT SPECIES    f
                PROPORTION OF LARGE  RIVER  SPECIES INDIVIDUALS TO TOTAL COONT    0.0%
                  PROPORTION OF HEADWATER  SPECIES INDIVIDUALS TO TOTAL COUNT    0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    92.1%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT    52.9%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    21.5%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT    0.0%
                                       PROPORTION OF PIONEERS TO TOTAL COUNT    79.S%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    3.7%
                                                   PERCENT D.E.L.T. ANOMOLIES    0.0%

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                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               BDRON-KRIB LAXX PLAIN ECORSGION

                                          TPS SAMPLE #:TS91069    IDEM #-.91069.0

SITE:HOFF1OH DITCH             COUNTY:ALLK»        LOCATION:lttPLBS RD.  (D/S)                   COLLECTION DATE:OX-Jul-199
TIME:15S2hr  LATITUDE/LONGITUDE:41'  0' 23.0°/84' 57"  2.0°      DRAINAGB:sq. miles
SBGMENT:21-ST. MARYS RIVER                                  NATURAL REGION CODE:SC-BLUFFTON  TILL PLAIN SECTION
TOPOMAP: MAPLES                      HYDROLOGIC UNIT:4100005-MAUMEE RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR: LONGLINE      NET MESH: 0.318cm  SAMPLING DISTANCE :SO.meters
                             VOLTS:200  DC  CAPTURE DEPTH:< 0.30meters  DISTANCE TO SHORE:5.Ometers

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   233
                                                TOTAL NUMBER OF TAXA PRESENT    10
                                               NUMBER OF LARGE RIVER SPECIES     £
                                                    NUMBER OF DARTER SPECIES     2
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     2
                                                   NUMBER OF SUNFISH SPECIES     1
                                                 NUMBER OF HEADWATER SPECIES     (>
                                                    NUMBER OF MINNOW SPECIES     5
                                         NUMBER OF ROUND BODY SUCKER SPECIES     0
                                              NUMBER OF SUCKER FAMILY SPECIES     1
                                                 NUMBER OF SENSITIVE SPECIES     0
                                                  NUMBER  OF TOLERANT SPECIES     5
                 PROPORTION OF  LARGE RIVER  SPECIES  INDIVIDUALS TO  TOTAL COUNT     0.0%
                   PROPORTION OF  HEADWATER  SPECIES  INDIVIDUALS TO  TOTAL COUNT     0.0%

                                       PROPORTION OF  TOLERANTS TO  TOTAL COUNT     85.0%
                                       PROPORTION OF  OMNIVORES TO  TOTAL COUNT     68.2%
                                    PROPORTION OF INSECTIVORES TO  TOTAL COUNT     14.2%
                                      PROPORTION OF CARNIVORES TO  TOTAL COUNT      0.0%
                                        PROPORTION  OF PIONEERS TO  TOTAL COUNT     90.6%

                               PROPORTION OF SIMPLE LITHOPHILS  TO  TOTAL COUNT     2.1%
                                                   PERCENT D.E.L.T.  ANOMOLIES      0.0%

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                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF MATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-ERIE LAKE PLAIN ECORBGIOH

                                          TPS SAMPLE #:TS91070    IDEM #:91070.0

3ITE.-HOFFKMI CREEK             COUNTY:ALLEN        LOCATION:TERHKT RD. (D/S)                  COLLECTION DATE: 01-Jul-1991
CIME:1623hr  LATITUDE/LONGITUDE:41'  \«  0^0°/841 53"  30.0°      DRAINAGE: 17^93 q. miles
SEGMENT:21-ST.  MARYS RIVER                                  NATURAL REGION CODE:4-NORTHERN LAKES NATURAL REGION
[•OPOMAP:  MAPLES                      HYDROLOGIC UNIT:4100005-MAOMEE RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE: IQOmeters
                             VOLTS :3JM)  DC  CAPTURE DEPTH: < 1.20meters  DISTANCE TO  SHORE :4 . Smeters

                                         TOTAL NUMBER  OF INDIVIDUALS COUNTED   167
                                                TOTAL  NUMBER OF TAXA PRESENT    15
                                               NUMBER  OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     £
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     1
                                                   NUMBER OF SUNFISH SPECIES     2
                                                 NUMBER OF HEADWATER SPECIES     0_
                                                    NUMBER OF MINNOW SPECIES     £
                                         NUMBER OF ROUND BODY SUCKER SPECIES     0
                                             NUMBER OF SUCKER FAMILY SPECIES     1
                                                 NUMBER OF SENSITIVE SPECIES     <>
                                                  NUMBER OF TOLERANT SPECIES     9_
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0*
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    70.1%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT     7.8%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT     82.6%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT     0.6%
                                        PROPORTION OF PIONEERS TO TOTAL COUNT     31.7%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT     6.0%
                                                  PERCENT D.E.L.T. ANOMOLIES     0.0%

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                                         INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-KRIK LAXB PIAXW RCOREGION

                                          TPS SAMPLE #:TS91071    IDEM #:91071.0

SITE:LITTLB BLUK CRHKK         COUNTY:ADAMS        LOCATIONS.R. SOOK                          COLLECTION DATE: 02-Jul-19!
TIME: 1102hr  LATITUDE/LONGITUDE:4£' 31"  21.0°/84' 4_9" 14.0"      DRAINAGE: 8_._3sq. miles
SEGMENT:21-ST. MARYS RIVER                                  NATURAL REGION CODE:5C-BLUFFTON TILL PLAIN SECTION
TOPOMAP: WILLSHIRE                   HYDROLOGIC UNIT-.4100004-ST. MARY'S RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR: LONGLINE      NET MESH: 0 . 318cm  SAMPLING DISTANCE :5_0meters
                             VOLTS :3JK>   DC  CAPTURE DEPTH:< 0.20meters  DISTANCE TO SHORE:3.Ometera

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   352
                                                TOTAL NUMBER OF TAXA PRESENT     7
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     1
                                                   NUMBER OF SUNFISH SPECIES     0
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     5
                                         NUMBER OF ROUND BODY SUCKER SPECIES     0
                                             NUMBER OF  SUCKER FAMILY SPECIES     1
                                                 NUMBER OF SENSITIVE SPECIES     1
                                                  NUMBER OF TOLERANT SPECIES     4
                PROPORTION  OF  LARGE RIVER  SPECIES  INDIVIDUALS TO TOTAL  COUNT     0.0%
                  PROPORTION OF  HEADWATER  SPECIES  INDIVIDUALS TO TOTAL  COUNT     0.0%

                                       PROPORTION OF  TOLERANTS TO TOTAL  COUNT     42.5%
                                       PROPORTION OF  OMNIVORES TO TOTAL  COUNT     38.1%
                                    PROPORTION  OF  INSECTIVORES TO TOTAL  COUNT     57.5%
                                      PROPORTION OF CARNIVORES TO TOTAL  COUNT      0.0%
                                        PROPORTION  OF PIONEERS TO TOTAL  COUNT     82.5%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL  COUNT      6.3%
                                                   PERCENT  D.E.L.T.  ANOMOLIES      0.0%

-------
                                         INDIANA DEPARTMENT OF  ENVIRONMENTAL MANAGEMENT
                                                   OFFICE  OF WATER MANAGEMENT
                                                   BIOLOGICAL STUDIES  SECTION

                                              FISH COMMUNITY IBI  DEVELOPMENT DATA
                                                HURON-ERIE LAKE PLAIN  BCORBQION

                                          TPS SAMPLE 8:TS91072    IDEM #;91072.0

SITE:BLOE CREEK                COUNTYrADAMS         LOCATIONS.R.  200S (D/S)                    COLLECTION DATE: 18-Jul-1991
TIME:1015hr  LATITUDE/LONGITUDE :40.' 43_"   B^J)0/!!!'  £9"  37.0°      DRAINAGE:70.3sq.  miles
SEGMBNT:21-ST. MARYS RIVER                                   NATURAL REGION  CODE:5C-BLUFFTON TILL PLAIN SECTION
TOPOMAP: WILLSHIRE                   HYDROLOGIC UNIT:4100004-ST.  MARY'S RIVER  BASIN (MADMBE RIVER BASIN)

                             GEAR;LONGLINE       NET MESH: 0. 318cm  SAMPLING  DISTANCE: IQOmeters
                             VOLTS:3JX)   DC   CAPTURE DEPTH:< 0.30metera  DISTANCE TO SHORE:3 . 9meters

                                          TOTAL NUMBER  OF  INDIVIDUALS  COUNTED    242
                                                 TOTAL  NUMBER OF  TAXA  PRESENT     15
                                                NUMBER  OF  LARGE RIVER  SPECIES     0
                                                    NUMBER OF  DARTER  SPECIES     2
                                                    NUMBER OF  MADTOM  SPECIES     0
                                                    NUMBER OF SCULPIN  SPECIES     0
                                     NUMBER  OF DARTER/MADTOM/SCULPIN  SPECIES     2
                                                    NUMBER OF SUNFISH  SPECIES     1
                                                  NUMBER OF HEADWATER  SPECIES     0
                                                    NUMBER OF  MINNOW  SPECIES     9
                                          NUMBER OF ROUND  BODY  SUCKER  SPECIES     £
                                              NUMBER OF SUCKER  FAMILY  SPECIES     2
                                                  NUMBER OF SENSITIVE  SPECIES     1
                                                   NUMBER  OF TOLERANT  SPECIES     £
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS  TO TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS  TO TOTAL COUNT     0.0%

                                      PROPORTION OF TOLERANTS  TO TOTAL COUNT     58.3%
                                      PROPORTION OF OMNIVORES  TO TOTAL COUNT     32.2%
                                   PROPORTION OF INSECTIVORES  TO TOTAL COUNT     52.1%
                                     PROPORTION OF CARNIVORES  TO TOTAL COUNT     0.0%
                                       PROPORTION OF PIONEERS  TO TOTAL COUNT     59.9%

                              PROPORTION OF  SIMPLE LITHOPHILS  TO TOTAL COUNT     19.8%
                                                   PERCENT D.E.L.T.  ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OP ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IB! DEVELOPMENT DATA
                                                          IAKB PLAXH BCOREOION
                                          TPS  SAMPLE  8:TS91073     IDEM  #:91073.0

SITE:BLUB CRBBK                 COUNTY : ADAMS         LOCATION: 100K  (U/S)                         COLLECTION DATE : 02- Jul-199
TIME:1146hr  LATITUDE/LONGITUDE :40'  41"  50.0<>/84'  55"   2.0°       DRAINAGE : 2 2. 3 sq.  miles
SEGMENT: 21 -ST. MARYS RIVER                                   NATURAL REGION CODE : 5C-BLUFFTON TILL PLAIN SECTION
TOPOMAP: BERNE                       HYDROLOGIC ONIT:4100004-ST.  MARY'S RIVER BASIN (MADMEE RIVER BASIN)

                              GEAR : LONGLINE       NET MESH: 0.3 18cm   SAMPLING DISTANCE :50meters
                              VOLTS :3_00_   DC   CAPTURE DEPTH :<  0.20meters   DISTANCE  TO SHORE : 4 . Ometer 8

                                          TOTAL NUMBER OF INDIVIDUALS COUNTED   503
                                                 TOTAL NUMBER OF TAXA PRESENT   11
                                                NUMBER OF LARGE  RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     £
                                                    NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/ SCULPIN SPECIES     1
                                                    NUMBER OF SUNFISH SPECIES     1
                                                  NUMBER OF HEADWATER SPECIES     0_
                                                    NUMBER OF MINNOW SPECIES     £
                                          NUMBER OF ROUND BODY SUCKER SPECIES     0
                                              NUMBER OF SUCKER FAMILY SPECIES     1
                                                  NUMBER OF SENSITIVE SPECIES     0
                                                   NUMBER OF  TOLERANT SPECIES     £
                 PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                   PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0V

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    80.1%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT    64.4%
                                     PROPORTION OF INSECTIVORES TO TOTAL COUNT    15.7%
                                      PROPORTION OF CARNIVORES TO TOTAL COUNT     0.0%
                                         PROPORTION OF  PIONEERS TO TOTAL COUNT    85.1%

                                PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    12.3%
                                                    PERCENT D.E.L.T.  ANOMOLIES      0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-ERIK LAKE PLAIN KCOREQION

                                          TPS SAMPLE #:TS91074    IDEM #:91074.0

5ITE:HABJCGGKR DITCH            COUNTY:ADM«S        LOCATION:C.R. 100E  (U/S)                    COLLECTION DATE: 02-Jul-1991
riME:1208hr  LATITUDE/LONGITUDE :4J3' 40" 30.0°/84' 55"  2.0°      DRAINAGE :7_ilsq- miles
SEGMENT:21-ST. MARYS RIVER                                  NATURAL REGION CODE:5C-BLUFFTON TILL PLAIN SECTION
fOPOMAP:  BERNE                       HYDROLOGIC UNIT:4100004-ST. MARY'S RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE:SOmetera
                             VOLTS:300  DC  CAPTURE DEPTH:< 0.20meters  DISTANCE TO  SHORE:3.9meters

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED     £
                                                TOTAL NUMBER OF TAXA PRESENT     2
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     0
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0_
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     0
                                                   NUMBER OF SUNFISH SPECIES     t)
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     2
                                         NUMBER OF ROUND BODY SUCKER SPECIES     0
                                             NUMBER OF SUCKER FAMILY SPECIES     0
                                                 NUMBER OF SENSITIVE SPECIES     0
                                                  NUMBER OF TOLERANT SPECIES     2
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    100.0%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT     50.0%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT     0.0%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT     0.0%
                                        PROPORTION OF  PIONEERS TO TOTAL COUNT     50.0%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT     0.0%
                                                  PERCENT  D.E.L.T.  ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               BORON-BRIE LAKX PLAIN BCORK3IOM

                                          TPS SAMPLE #:TS9107S

SITE:BLOT CRUX                COUNTY:ADMIS        LOCATIONS.R. 100S  (U/S)                    COLLECTION DATE:02-Jul-199
TIME:1239hr  LATITODE/LONGITUDB:4£' 43" 32.0°/85'  2"   6.0"      DRAINAGE: 17.33d. miles
SEGMENT:21-ST. MARYS RIVER                                  NATURAL REGION CODB:5C-BLIIFFTON  TILL PLAIN SECTION
TOPOMAP: LINN GROVE                  HYDROLOGIC UNIT:4100004-ST. MARY'S RIVER BASIN (MAUMEE  RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE :5p_meters
                             VOLTS:3£0  DC  CAPTURE DEPTH:< 0.60meter3  DISTANCE TO SHORE: 4 . Procters

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    144
                                                TOTAL NUMBER OF TAXA PRESENT     8
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     0
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     £
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     0
                                                   NUMBER OF SUNFISH SPECIES     1
                                                 NUMBER OF HEADWATER SPECIES     0_
                                                    NUMBER OF MINNOW SPECIES     5
                                         NUMBER OF ROUND BODY SUCKER SPECIES     £
                                             NUMBER OF  SUCKER FAMILY SPECIES     0
                                                 NUMBER OF SENSITIVE SPECIES     0
                                                  NUMBER OF TOLERANT SPECIES     7
                 PROPORTION OF LARGE RIVER  SPECIES  INDIVIDUALS TO  TOTAL COUNT     0.0%
                   PROPORTION OF  HEADWATER  SPECIES  INDIVIDUALS TO  TOTAL COONT     0.0%

                                       PROPORTION OF TOLERANTS TO  TOTAL COUNT     80.6%
                                       PROPORTION OF OMNIVORE£ TO  TOTAL COUNT     70.8%
                                    PROPORTION OF INSECTIVORES TO  TOTAL COUNT     29.2%
                                      PROPORTION OF CARNIVORES TO  TOTAL COUNT      0.0%
                                        PROPORTION OF  PIONEERS TO  TOTAL COUNT     59.0%

                               PROPORTION OF SIMPLE LITHOPHILS TO  TOTAL COUNT      0.0%
                                                   PERCENT D.E.L.T.  ANOMOLIES      0.0%

-------
                                         INDIANA DEPARTMENT OF  ENVIRONMENTAL MANAGEMENT
                                                   OFFICE  OF WATER MANAGEMENT
                                                   BIOLOGICAL STUDIES SECTION

                                              PISH COMMUNITY IBI  DEVELOPMENT DATA
                                                HURON-KRIB LAKE PLAIN BCOKBGION

                                           TPS SAMPLE #:TS91076    IDEM 0:91076.0

SITE:MARTZ CRSXK               COONTY:ADA1IS         LOCATIONS.R.  100H (0/S)                    COLLECTION DATE:02-Jul-1991
TIME:1416hr  LATITUDE/LONGITUDE:4J)1  45."  40.Q''/84'  52"  21.0°      DRAINAGE :8i8sq. miles
SEGMENT:21-ST. MARYS RIVER                                   NATURAL REGION CODE:5C-BLUFFTON TILL PLAIN SECTION
TOPOMAP: WREN                        HYDROLOGIC UNIT:4100004-ST.  MARY'S RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH: 0 . 318cm  SAMPLING DISTANCE: SOmeters
                             VOLTS: 3.00   DC  CAPTURE DEPTH:< 0.30meter3  DISTANCE TO SHORE:2.3meters

                                         TOTAL NUMBER  OF  INDIVIDUALS COUNTED    4S.
                                                 TOTAL  NUMBER OF  TAXA PRESENT     7
                                                NUMBER  OF  LARGE RIVER SPECIES     0
                                                     NUMBER OF  DARTER SPECIES     0
                                                     NUMBER OF  MADTOM SPECIES     0
                                                    NUMBER OF SCULPIN SPECIES     0
                                     NUMBER  OF DARTER/MADTOM/SCULPIN SPECIES     0
                                                    NUMBER OF SUNFISH SPECIES     1
                                                  NUMBER OF HEADWATER SPECIES     0
                                                     NUMBER OF  MINNOW SPECIES     3
                                         NUMBER OF ROUND  BODY  SUCKER SPECIES     0
                                              NUMBER OF SUCKER  FAMILY SPECIES     0
                                                  NUMBER OF SENSITIVE SPECIES     0
                                                   NUMBER  OF TOLERANT SPECIES     4
                PROPORTION  OF  LARGE  RIVER  SPECIES INDIVIDUALS  TO TOTAL COUNT     0.0%
                  PROPORTION OF  HEADWATER  SPECIES INDIVIDUALS  TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS  TO TOTAL COUNT    85.7%
                                       PROPORTION OF OMNIVORES  TO TOTAL COUNT    69.4%
                                   PROPORTION OF INSECTIVORES  TO TOTAL COUNT    26.5%
                                     PROPORTION OF CARNIVORES  TO TOTAL COUNT     2.0%
                                        PROPORTION OF PIONEERS  TO TOTAL COUNT    81.6%

                               PROPORTION OF  SIMPLE LITHOPHILS  TO TOTAL COUNT     0.0%
                                                   PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OP ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HORON-KR1B LAKE PLAIN BCORKG10N

                                          TPS SAMPLE #:TS910T7

SITErYKLLOW CREEK              COUNTY:ADMIS        LOCATION:C.R. 100H  (0/S)                    COLLECTION DATE: 02-Jul-199
TIME:1419hr  LATITODE/LONGITUDE:4CP 45" 34.0°/84' 55." 54.0°      DRAINAGE: 12.2sg. miles
SEGMENT:21-ST. MARYS RIVER                                  NATURAL REGION CODE:5C-BLUFFTON TILL PLAIN SECTION
TOPOMAP: DECATDR                     HYDROLOGIC UNIT:4100004-ST. MARY'S RIVER  BASIN (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH: 0 . 318cm  SAMPLING DISTANCE:50meters
                             VOLTS :3_00  DC   CAPTURE DEPTH :< 0.30meters  DISTANCE TO SHORE :4 . 9meters

                                          TOTAL NUMBER OF INDIVIDUALS COUNTED   129
                                                TOTAL NUMBER OF TAXA PRESENT     4
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     0
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER  OF DARTER/MADTOM/SCDLPIN SPECIES     0_
                                                   NUMBER OF SUNFISH SPECIES     0
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     3_
                                          NUMBER OF ROUND BODY SUCKER SPECIES     0
                                              NUMBER OF  SUCKER FAMILY SPECIES     £
                                                 NUMBER OF SENSITIVE SPECIES      0
                                                  NUMBER OF TOLERANT SPECIES     2
                 PROPORTION OF  LARGE RIVER SPECIES  INDIVIDUALS TO TOTAL COUNT      0.0%
                   PROPORTION OF HEADWATER SPECIES  INDIVIDUALS TO TOTAL COUNT      0.0%

                                       PROPORTION OF  TOLERANTS TO TOTAL COUNT     96.1%
                                       PROPORTION OF  OMNIVORES  TO TOTAL COUNT     96.1%
                                    PROPORTION OF INSECTIVORES  TO TOTAL COUNT      3.9%
                                      PROPORTION OF CARNIVORES  TO TOTAL COUNT      0.0%
                                        PROPORTION OF PIONEERS  TO TOTAL COUNT    96.1%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT     0.0%
                                                   PERCENT D.E.L.T.  ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-KRIK LAKE PLAIN KCOKBGXON

                                          TPS SAMPLE #:TS91078

3ITE:HOLTHOUSK DITCH           COUNTY:ADAMS        LOCATIONS.R. 400H  (D/S)                    COLLECTION DATE:02-Jul-1991
riME:I550hr  LATITUDE/LONGITUDE-.40' 48"  6^0°/8^'  2"  7.0"      DRAINAGE:23.Isq. miles
3EGMENT:21-ST. MARYS RIVER                                  NATURAL REGION CODE:5C-BLOFFTON TILL  PLAIN SECTION
rOPOMAP:  PREBLE                      HYDROLOGIC UNIT = 4100004-ST. MARY'S RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH: 0.318cm  SAMPLING DISTANCE:50meters
                             VOLTS :300_  DC  CAPTURE DEPTH:< 0.20meters  DISTANCE TO  SHORE:2 . Smeters

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   166
                                                TOTAL NUMBER OF TAXA PRESENT    10
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     £
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     1
                                                   NUMBER OF SUNFISH SPECIES     1
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     5
                                         NUMBER OF ROUND BODY SUCKER SPECIES     0
                                             NUMBER OF SUCKER FAMILY SPECIES     1
                                                 NUMBER OF SENSITIVE SPECIES     0
                                                  NUMBER OF TOLERANT SPECIES     6_
                PROPORTION OF LARGE RIVER SPECIES  INDIVIDUALS TO TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES  INDIVIDUALS TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT     81.9%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT     70.5%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT     24.1%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT     0.0%
                                        PROPORTION  OF  PIONEERS TO TOTAL COUNT     58.4%

                              PROPORTION OF  SIMPLE LITHOPHILS TO TOTAL COUNT    30.7%
                                                   PERCENT D.E.L.T.  ANOMOLIES     0.0%

-------
                                         INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-DUX LAKE PLAIN BCORBGION

                                          TPS SAMPLE #:TS91079

SITEtBOKUM RDM                 COUNTY:ADMIS        LOCATIONtC.R. 400H                          COLLECTION DATE: 02-Jul-199
TIME:1624hr  LATITUDE/LONGITUDE: 40 ' 48."  10.0°/84' 5£" 24.0°      DRAINAGE: 7. lag. miles
SEGMENT:21-ST. MARYS RIVER                                  NATURAL REGION CODE:5C-BLUFFTON TILL PLAIN SECTION
TOPOMAP: DECATUR                     HYDROLOGIC UNIT-.4100004-ST. MARY'S RIVER  BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH: 0 . 318cm  SAMPLING DISTANCE :£0meters
                             VOLTS:300   DC  CAPTURE DEPTH:< 0.20meter3  DISTANCE TO  SHORE:2.4meters

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   238
                                                TOTAL NUMBER OF TAXA PRESENT    11
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     2
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     2.
                                                   NUMBER OF SUNFISH SPECIES     0
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     6
                                         NUMBER OF ROUND BODY SUCKER SPECIES     £
                                             NUMBER OF SUCKER FAMILY SPECIES     1
                                                 NUMBER OF SENSITIVE SPECIES     0
                                                  NUMBER OF TOLERANT SPECIES     5
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    80.7%
                                       PROPORTION OF OMNIVOREr TO TOTAL COUNT    69.7%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    10.1%
                                      PROPORTION OF CARNIVORES TO TOTAL COUNT     0.0%
                                        PROPORTION OF  PIONEERS TO TOTAL COUNT    90.3%

                              PROPORTION OF  SIMPLE LITHOPHILS TO TOTAL COUNT     8.4%
                                                  PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-KRIS LAKB PLAIN ECORBQION

                                                                  IDEM #:91080.0

:iTE:MAtJMBB RTVKR               COUNTY:ALLES        LOCATIONS.5 mi N FT.  WKXNB                COLLECTION DATE:03-Jul-1991
•IME:9_OOhr   LATITUDE/LONGITUDE: 41'   4"  56.0°/8S'  6." 52.0"      DRAINAGE: •••. *sq.  miles
!EGMEHT:19-MAUMEE RIVER (MAIN STEM)                          NATURAL REGION CODE:5C-BLUFFTON TILL PLAIN SECTION
•OPOMAP:  FORT WAYNE  EAST             HYDROLOGIC UNIT:4100005-MADMEE RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:SPORT  YAK II  NET MESH:0.316cm  SAMPLING DISTANCE:400meters
                             VOLTS :3JK>  DC  CAPTURE DEPTH: < 1.3Procters  DISTANCE TO SHORE:79 .2meters

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   319
                                                TOTAL NUMBER OF TAXA PRESENT    33.
                                               NUMBER OF LARGE RIVER SPECIES     5
                                                    NUMBER OF DARTER SPECIES     4
                                                    NUMBER OF MADTOM SPECIES     1
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     5
                                                   NUMBER OF SUNFISH SPECIES     6
                                                 NUMBER OF HEADWATER SPECIES     1
                                                    NUMBER OF MINNOW SPECIES    10
                                         NUMBER OF ROUND BODY SUCKER SPECIES     3_
                                             NUMBER OF SUCKER FAMILY SPECIES     5
                                                 NUMBER OF SENSITIVE SPECIES    10
                                                  NUMBER OF TOLERANT SPECIES     8
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT    17.9%
                 PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.9%

                                      PROPORTION OF TOLERANTS TO TOTAL COUNT    55.2%
                                      PROPORTION OF OMNIVORES TO TOTAL COUNT    48.6%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    42.9%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT     4.4%
                                       PROPORTION OF PIONEERS TO TOTAL COUNT    19.4%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT     8.2%
                                                  PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HORON-RRIB LAKX PLAIN BCOKBGION

                                          TPS SAMPLE #:TS910B1    IDEM #:91081.0

SITE:HOTK DITCH                COUNTY:AIiBK        LOCATION:FLXTROCX ROAD BRIDGE               COLLECTION DATE:08-Jul-19S
TIME:1400hr  LATITUDE/LONGITUDE :40' 57" 44.0°/8S'  0" 25.0°      DRAINAGE: 10.3 SO. miles
SEGMENT:21-ST. MARYS RIVER                                  NATURAL REGION CODE:5C-BLUFFTON TILL PLAIN SECTION
TOPOMAP: POE                         HYDROLOGIC ONIT:4100004-ST. MARY'S RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0. 318cm  SAMPLING DISTANCE:BOmeters
                             VOLTS:3_0_0  DC   CAPTURE DEPTH:< O.VOmeters  DISTANCE TO  SHORE-. 1.9meters

                                          TOTAL NUMBER OF INDIVIDUALS COUNTED   379
                                                TOTAL NUMBER OF TAXA PRESENT    12
                                               NUMBER OF LARGE RIVER SPECIES     0_
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER  OF DARTER/MADTOM/SCULPIN SPECIES     1
                                                   NUMBER OF SUNFISH SPECIES     2
                                                  NUMBER OF  HEADWATER SPECIES     0
                                                    NUMBER  OF MINNOW SPECIES     5
                                          NUMBER  OF ROUND BODY SUCKER SPECIES     JO
                                              NUMBER OF  SUCKER FAMILY SPECIES     1
                                                  NUMBER OF  SENSITIVE SPECIES      0
                                                   NUMBER OF TOLERANT SPECIES      6
                 PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     JL,0%
                   PROPORTION OF  HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT      0.0*

                                       PROPORTION OF TQLERANTS  TO TOTAL COUNT     72,8%
                                       PROPORTION OF OMNIVORES  TO TOTAL COUNT    40.6%
                                    PROPORTION OF INSECTIVORES  TO TOTAL COUNT    12.4%
                                      PROPORTION OF CARNIVORES  TO TOTAL COUNT     0.3%
                                        PROPORTION OF PIONEERS  TO TOTAL COUNT    93.7%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT     2.1%
                                                   PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                              FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-ERIB LAKE PLAIN BCORKGIOH

                                          TPS SAMPLE #:TS91082

SITE.-HAHBKR DITCH              COUNTY:ALLKN         LOCATION:HAmLTOH RD.  (D/S)                 COLLECTION DATE:08-Jul-1991
TIME:1500hr  LATITUDE/LONGITUDE:4J3'  5£" 44.0°/85' 10"  53.0°      DRAINAGE:77l.Oaq.  miles
SEGMENT: 21-ST. MARYS RIVER                                  NATURAL REGION CODE: SC-BLUFFTON TILL PLAIN SECTION
TOPOMAP: OSS IAN                      HYDROLOGIC UNIT:4100004-ST. MARY'S RIVER BASIN (MAOMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE :5_0meters
                             VOLTS :3JM)  DC   CAPTURE DEPTH:< 1. OOmeters  DISTANCE  TO SHORE :2 .4meters

                                          TOTAL NUMBER  OF INDIVIDUALS COUNTED    6T_
                                                TOTAL  NUMBER OF TAXA PRESENT      8
                                               NUMBER  OF LARGE RIVER SPECIES      0
                                                    NUMBER OF DARTER SPECIES      0.
                                                    NUMBER OF MADTOM SPECIES      0
                                                    NUMBER OF SCULPIN SPECIES      0
                                     NUMBER  OF DARTER/MADTOM/SCULPIN SPECIES      0
                                                    NUMBER OF  SONFISH SPECIES      1
                                                  NUMBER OF HEADWATER SPECIES      0_
                                                    NUMBER OF MINNOW SPECIES      4
                                          NUMBER OF  ROUND BODY SUCKER SPECIES      0
                                              NUMBER OF SUCKER FAMILY SPECIES      1
                                                  NUMBER OF SENSITIVE SPECIES      0
                                                  NUMBER OF  TOLERANT SPECIES      5
                 PROPORTION  OF  LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                   PROPORTION OF  HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    82.1%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT    26.9%
                                    PROPORTION OF INSECTIVORES TO TOTAL COUNT    20.9%
                                      PROPORTION OF CARNIVORES TO TOTAL COUNT     1.5%
                                        PROPORTION OF PIONEERS TO TOTAL COUNT    74.6%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    19.4%
                                                   PERCENT D.E.L.T.  ANOMOLIES     0.0%

-------
                                         INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                   OFFICE  OF WATER MANAGEMENT
                                                   BIOLOGICAL STUDIES  SECTION

                                              FISH COMMUNITY IBI  DEVELOPMENT DATA
                                                HURON-ERIE LAKE PLAIN  BCORTOION

                                           TPS SAMPLE #:TS91084

SITE:HQOK DITCH                COUNTY:ALLK»T        LOCATION:MARION CEHTKR HD.  (B/S)            COLLECTION DATE:08-Jul-199
TIME:1616hr  LATITUDE/LONGITUDE: 4_0'  52"  55.0°/85'   31"  23.0°      DRAINAGE: 13 .2so.  miles
SEGMENT:21-ST. MARYS RIVER                                   NATURAL REGION  CODE:5C-BUJFFTON TILL PLAIN SECTION
TOPOMAP: POE                          HYDROLOGIC UNIT:4I00004-ST.  MARY'S  RIVER  BASIN (MAUMEE RIVER BASIN)

                             GEAR-.LONGLINE      NET MESH:0.318em  SAMPLING  DISTANCE:SOmeters
                             VOLTS :3_00   DC  CAPTURE DEPTH:< 0. SOmeters   DISTANCE TO SHORE: 3 . Ometers

                                          TOTAL NUMBER  OF  INDIVIDUALS  COUNTED   289
                                                 TOTAL  NUMBER OF  TAXA  PRESENT    21
                                                NUMBER  OF  LARGE RIVER  SPECIES     1
                                                    NUMBER OF DARTER  SPECIES     2
                                                    NUMBER OF MADTOM  SPECIES     0
                                                    NUMBER OF SCULPIN  SPECIES     0
                                      NUMBER OF DARTER/MADTOM/SCULPIN  SPECIES     2
                                                    NUMBER OF SUNFISH  SPECIES     4
                                                  NUMBER OF HEADWATER  SPECIES     0
                                                    NUMBER OF MINNOW  SPECIES     9_
                                          NUMBER OF ROUND  BODY SUCKER  SPECIES     0_
                                              NUMBER OF SUCKER FAMILY  SPECIES     2_
                                                  NUMBER OF SENSITIVE  SPECIES     1
                                                   NUMBER  OF TOLERANT  SPECIES     9
                 PROPORTION  OF  LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.3V
                   PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    74.0%
                                       PROPORTION OF OMNIVOREE TO TOTAL COUNT    37.0%
                                    PROPORTION OF INSECTIVORES TO TOTAL COUNT    20.4%
                                      PROPORTION OF CARNIVORES TO TOTAL COUNT     1.7%
                                        PROPORTION OF  PIONEERS TO TOTAL COUNT    76.5%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT     9.0%
                                                   PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-BRIB LAKH PLAIN ECORBGION

                                          TPS SAMPLE ft:TS91100    IDEM ft:91100.0

!ITE:CKUAR CRBKK               COUNTY: DKKALB       LOCATIONS.R. 27 (U/S)                     COLLECTION  DATE: 11-Jul-1991
'IME:84_Ohr   LATITUDE/LONGITUDE :41'  26_" 51.0°/8S'  31" 48.0"      DRAINAGE: 2 4.8 sq. miles
iEGMENT: 18-CEDAR CREEK                                      NATURAL REGION CODE.-SC-BLUFFTOH TILL PLAIN SECTION
•OPOMAP: WATERLOO                    HYDROLOGIC UNIT:4100003-ST. JOSEPH RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE: IQOmeters
                             VOLTS rJJM)   DC  CAPTURE DEPTH :< O.SOmeters  DISTANCE TO SHORE:5.5meters

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    83
                                                TOTAL NUMBER OF TAXA PRESENT    17
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     4
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     4
                                                   NUMBER OF SUNFISH SPECIES     1
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     3_
                                         NUMBER OF ROUND BODY SUCKER SPECIES     1
                                             NUMBER OF SUCKER FAMILY SPECIES     2
                                                 NUMBER OF SENSITIVE SPECIES     4
                                                  NUMBER OF TOLERANT SPECIES     5
                PROPORTION  OF LARGE  RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                 PROPORTION  OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                     PROPORTION OF TOLERANTS TO TOTAL COUNT    44.6%
                                     PROPORTION OF OMNIVORES TO TOTAL COUNT     6.0%
                                  PROPORTION OF INSECTIVORES TO TOTAL COUNT    83.1%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT    10.8%
                                       PROPORTION OF PIONEERS TO TOTAL COUNT    37.3%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    28.9%
                                                  PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HDRON-BRIX LAKX PLAIN BCOREGIOH

                                          TPS SAMPLE #:TS91101    IDEM  #:91101.0

SITE:MATSAN DITCH              COUNTY:DXEALB       LOCATION-.S .R. 6  (D/S)                       COLLECTION DATE: ll-Jul-199
TIME:j>3_Ohr   LATITUDE/LONGITUDE: 41' 25" 52.0''/84I 5£" 50.0°      DRAINAGE: 15. 4sa.  miles
SEGMENT: 18-CEDAR CREEK                                      NATURAL REGION CODE:5C-BLUFPTON TILL PLAIN SECTION
TOPOMAP: WATERLOO                    HYDROLOGIC UNIT:4100003-ST. JOSEPH RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE;IQOmeters
                             VOLTS:10£  2£  CAPTURE DEPTH:< 1. OOmeters  DISTANCE TO SHORE: 5 . 3meters

                                          TOTAL NUMBER OF INDIVIDUALS COUNTED    111
                                                TOTAL NUMBER OF TAXA PRESENT     12
                                               NUMBER OF LARGE RIVER SPECIES      0
                                                    NUMBER OF DARTER SPECIES      2.
                                                    NUMBER OF MADTOM SPECIES      p.
                                                   NUMBER OF SCULPIN SPECIES      (>
                                     NUMBER OF DARTER/MADTOM/ SCULPIN SPECIES      2
                                                   NUMBER OF SUNFISH SPECIES      1
                                                 NUMBER OF HEADWATER SPECIES      2
                                                    NUMBER OF MINNOW SPECIES      6
                                          NUMBER OF ROUND BODY SUCKER SPECIES      £
                                             NUMBER OF  SUCKER FAMILY SPECIES      1
                                                 NUMBER OF SENSITIVE SPECIES      0
                                                  NUMBER OF TOLERANT SPECIES      £
                 PROPORTION OF LARGE RIVER SPECIES  INDIVIDUALS TO TOTAL  COUNT     0.0%
                   PROPORTION OF HEADWATER SPECIES  INDIVIDUALS TO TOTAL  COUNT    11.7%

                                       PROPORTION OF TOLERANTS TO TOTAL  COUNT    80.2%
                                       PROPORTION OF OMNIVORES TO TOTAL  COUNT    31.5%
                                    PROPORTION OF  INSECTIVORES TO TOTAL  COUNT    18.9%
                                      PROPORTION OF CARNIVORES TO TOTAL  COUNT     1.8%
                                        PROPORTION  OF  PIONEERS TO TOTAL  COUNT    SI.4%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL  COUNT    44.1%
                                                   PERCENT D.E.L.T.  ANOMOLIES     0.0%

-------
                                         INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                   OFFICE OF WATER MANAGEMENT
                                                   BIOLOGICAL STUDIES SECTION

                                              FISH COMMUNITY IBI  DEVELOPMENT DATA
                                                BORON-KRIS LAKE PLAIN KCOKEQION

                                           TPS SAMPLE #:TS91102

SITE:FISH CRKKK                 COUNTY: STBUBKH      LOCATION:CR 775 S                          COLLECTION DATE: ll-Jul-1991
TIME:1005hr  LATITUDE/LONGITUDE:41.'  31."  55.00/84'  54."  9.0°      DRAINAGE: 37 .Sag.  miles
SEGMENT:20-ST. JOSEPH RIVER                                  NATURAL REGION CODE:4-NORTHERN LAKES NATURAL REGION
TOPOMAP: HAMILTON                     HYDROLOGIC UNIT:4100003-ST.  JOSEPH RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0. 318cm  SAMPLING DISTANCE:IQOmeters
                             VOLTS :3_00   DC  CAPTURE DEPTH :< 0.70meters  DISTANCE TO SHORE: 5 . 2meters

                                          TOTAL NUMBER OF INDIVIDUALS COUNTED   161
                                                 TOTAL NUMBER OF  TAXA PRESENT    17
                                                NUMBER OF LARGE RIVER SPECIES     0
                                                     NUMBER OF DARTER SPECIES     2
                                                     NUMBER OF MADTOM SPECIES     0
                                                    NUMBER OF SCULPIN SPECIES     0_
                                      NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     2
                                                    NUMBER OF SUNFISH SPECIES     4
                                                  NUMBER OF HEADWATER SPECIES     0
                                                     NUMBER OF MINNOW SPECIES     2
                                          NUMBER OF ROUND BODY SUCKER SPECIES     2.
                                              NUMBER OF SUCKER FAMILY SPECIES     2.
                                                  NUMBER OF SENSITIVE SPECIES     £
                                                   NUMBER OF TOLERANT SPECIES     5
                 PROPORTION OF  LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                   PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    68.9%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT    52.2%
                                    PROPORTION OF INSECTIVORES TO TOTAL COUNT    31.7%
                                      PROPORTION OF CARNIVORES TO TOTAL COUNT    14.9%
                                        PROPORTION OF PIONEERS TO TOTAL COUNT    18.0%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT     4.3%
                                                   PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                         INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-ERIK LAKE PLAIN BCORHOION

                                          TPS SAMPLE #:TS91103    IDEM #:91103.0

SITE.-FISH CREKE                COUNTY: DKEALB       LOCATION:CR 16                              COLLECTION DATE: 11-Jul-199
TIME:1045hr  LATITUDE/LONGITUDE :4_1' 2f)"  25.0''/84' £9" 34.0"      DRAINAGE: 98. Bsg.  miles
SEGMENT:20-ST. JOSEPH RIVER                                 NATURAL REGION CODE:SC-BLOTFTON TILL PLAIN SECTION
TOPOMAP: BOTLER EAST                 HYDROLOGIC UNIT:4100003-ST. JOSEPH RIVER BASIN (MAHMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE:IQOmeters
                             VOLTS:^00   DC  CAPTURE DEPTH:< 0.9Procters  DISTANCE TO SHORE:7.Smeters

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    183
                                                TOTAL NUMBER OF TA3CA PRESENT     19.
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     4
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     1
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     5
                                                   NUMBER OF SONFISH SPECIES     3_
                                                 NUMBER OF HEADWATER SPECIES     1
                                                    NUMBER OF MINNOW SPECIES     5
                                         NUMBER OF ROUND BODY SUCKER SPECIES     2
                                             NUMBER OF  SUCKER FAMILY SPECIES     3
                                                 NUMBER OF SENSITIVE SPECIES     6
                                                  NUMBER OF TOLERANT SPECIES     7
                PROPORTION OF  LARGE RIVER SPECIES  INDIVIDUALS TO TOTAL COUNT     0.0%
                   PROPORTION OF HEADWATER SPECIES  INDIVIDUALS TO TOTAL COUNT     1.1%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT     48.6%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT     29.5%
                                    PROPORTION  OF  INSECTIVORES TO TOTAL COUNT     SO.3%
                                      PROPORTION OF CARNIVORES TO TOTAL COUNT     10.4%
                                        PROPORTION  OF  PIONEERS TO TOTAL COUNT     43.7%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT     12.0%
                                                   PERCENT  D.E.L.T.  ANOMOLIES      0.0%

-------
                                         INDIANA DEPARTMENT OF  ENVIRONMENTAL MANAGEMENT
                                                   OFFICE  OF WATER MANAGEMENT
                                                   BIOLOGICAL STUDIES  SECTION

                                              FISH  COMMUNITY IBI DEVELOPMENT DATA
                                                BORON-ERIE LAKS PLAIN  ECOREGION

                                          TPS SAMPLE #:TS91104    IDEM  #:91104.0

3ITE:B10 RDM                   COUNTY:DKKALB       LOCATION:C.R.  75  (U/S)                      COLLECTION DATE:ll-Jul-1991
riME:1143hr  LATITUDE/LONGITUDE: 41.' 2_5"  48.0°/84'  50_" 37.0°       DRAINAGE: 2 8. lag, miles
SEGMENT:20-ST.  JOSEPH RIVER                                  NATURAL REGION CODE: SC-BLUFFTON TILL PLAIN SECTION
TOPOMAP:  BUTLER EAST                 HYDROLOGIC UNIT:4100003-ST.  JOSEPH RIVER  BASIN (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE       NET MESH: 0 . 318cm   SAMPLING DISTANCE:lOOmeters
                             VOLTS:300   DC  CAPTURE DEPTH:<  0.40meter3  DISTANCE TO SHORE:S.Ometers

                                         TOTAL NUMBER OF  INDIVIDUALS  COUNTED   215
                                                 TOTAL NUMBER OF TAXA  PRESENT     7
                                                NUMBER OF  LARGE RIVER  SPECIES     0
                                                    NUMBER OF  DARTER  SPECIES     0
                                                    NUMBER OF  MADTOM  SPECIES     0
                                                   NUMBER OF SCULPIN  SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN  SPECIES     0
                                                   NUMBER OF SUNFISH  SPECIES     1
                                                 NUMBER OF HEADWATER  SPECIES     1
                                                    NUMBER OF  MINNOW  SPECIES     5.
                                         NUMBER OF ROUND  BODY  SUCKER  SPECIES     £
                                              NUMBER OF SUCKER  FAMILY  SPECIES     1
                                                 NUMBER OF SENSITIVE  SPECIES     0
                                                  NUMBER  OF  TOLERANT  SPECIES     4
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS  TO  TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS  TO  TOTAL COUNT     20.0%

                                      PROPORTION OF TOLERANTS  TO  TOTAL COUNT     89.8%
                                      PROPORTION OF OMNIVORES  TO  TOTAL COUNT     2.8%
                                   PROPORTION OF INSECTIVORES  TO  TOTAL COUNT     26.S%
                                     PROPORTION OF CARNIVORES  TO  TOTAL COUNT     0.0%
                                       PROPORTION OF PIONEERS  TO  TOTAL COUNT     74.9%

                              PROPORTION OF SIMPLE LITHOPHILS  TO  TOTAL COUNT     25.1%
                                                  PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES  SECTION

                                              FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-KRIK LAKE PLAIN  BCORKGION

                                          TPS SAMPLE #:TS91105    IDEM  #:91105.0

SITE:BUCK CRBBX                COUNTY-.DKKALB       LOCATION :C.R. 40  (D/S)                      COLLECTION DATE: ll-Jul-199
TIME:1313hr  LATITUDE/LONGITUDE: 4_1' 23." 10.Q''/e4l 50." 47.0°      DRAINAGE:13.2aq.  miles
SEGMENT:20-ST. JOSEPH RIVER                                 NATURAL REGION CODE:5C-BLUFFTON TILL PLAIN SECTION
TOPOMAP: BUTLER EAST                 HYDROLOGIC UNIT:4100003-ST. JOSEPH RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE:IQOmeters
                             VOLTS :3_OJ)  DC  CAPTURE DEPTH: < 0.30meters  DISTANCE TO SHORE-.5 .Smeters

                                          TOTAL NUMBER OF INDIVIDUALS  COUNTED    248
                                                TOTAL NUMBER OF TAXA  PRESENT      £
                                               NUMBER OF LARGE RIVER  SPECIES      0
                                                    NUMBER  OF DARTER  SPECIES      2
                                                    NUMBER  OF MADTOM SPECIES      0
                                                   NUMBER OF SCULPIN SPECIES      0
                                      NUMBER OF DARTER/MADTOM/SCULPIN SPECIES      2
                                                   NUMBER OF  SUNFISH SPECIES      2
                                                  NUMBER OF  HEADWATER SPECIES      1
                                                     NUMBER  OF  MINNOW SPECIES     2
                                          NUMBER OF ROUND BODY SUCKER SPECIES     0
                                              NUMBER OF SUCKER FAMILY SPECIES     1
                                                  NUMBER OF SENSITIVE SPECIES     0
                                                   NOMBER OF TOLERANT SPECIES     5
                 PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                   PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     1^3%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    96.0%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT    42.7%
                                    PROPORTION OF INSBCTIVORES TO TOTAL COUNT    30.3%
                                      PROPORTION OF CARNIVORES TO TOTAL COUNT     0.0%
                                        PROPORTION OF PIONEERS TO TOTAL COUNT    54.0%

                               PROPORTION OF  SIMPLE LITHOPHILS TO TOTAL COUNT    12.1%
                                                   PERCENT D.E.L.T. ANOMOLIES      0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HORON-KRIK LAKE PLAIN BCORBGION

                                                                  IDEM #:91106.0

>ITE:SAL SHANK DITCH           COUNTY :DKCALB       LOCATIONS.R. 59 (U/S)                     COLLECTION DATE: 11-Jul-1991
?IME:1408hr  LATITUDE/LONGITUDE-.41'  21" 43.00/84' 54" 25.0"      DRAINAGE: 18^4sq. miles
iEGMENT:20-ST.  JOSEPH RIVER                                 NATURAL REGION CODE: 5C-BLUFFTON TILL PLAIN SECTION
'OPOMAP:  SAINT JOE                   HYDROLOGIC UNIT-.4100003-ST. JOSEPH RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE:5£raeters
                             VOLTS:300  DC  CAPTURE DEPTH:< 0.70meter3  DISTANCE TO SHORE:3.Ometers

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    42
                                                TOTAL NUMBER OF TAXA PRESENT     6
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     2
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     2
                                                   NUMBER OF SUNFISH SPECIES     1
                                                 NUMBER OF HEADWATER SPECIES     £
                                                    NUMBER OF MINNOW SPECIES     0
                                         NUMBER OF ROUND BODY SUCKER SPECIES     (>
                                             NUMBER OF SUCKER FAMILY SPECIES     1
                                                 NUMBER OF SENSITIVE SPECIES     0
                                                  NUMBER OF TOLERANT SPECIES     3_
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0*
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                      PROPORTION OF TOLERANTS TO TOTAL COUNT    90.5%
                                      PROPORTION OF OMNIVORES TO TOTAL COUNT    71.4%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    26.3%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT     2.4%
                                       PROPORTION OF PIONEERS TO TOTAL COUNT    21.4%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    73.8%
                                                  PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HORON-BRIB LAXB PIAHJ ECORKGION

                                          TPS SAMPLE #:TS91107

SITE:BKAR CHEEK                COUNTY: DKKALB       LOCATIONrC.R. 56 (0/S)                      COLLECTION DATE:ll-Jul-19S
TIME.-lSOOhr  LATITUDE/LONGITUDE:^1 .19" 41.0°/B4" 5jj" 33.0°      DRAINAGE: 22 .Tag, miles
SEGMENT:20-ST. JOSEPH RIVER                                 NATURAL REGION CODE:5C-BLUFFTON TILL PLAIN SECTION
TOPOMAP: SAINT JOE                   HYDROLOGIC UNIT-.4100003-ST. JOSEPH RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH: 0 .318cm  SAMPLING DISTANCE:50meters
                             VOLTS-.300  DC  CAPTURE DEPTH:< O.SOmeters  DISTANCE TO  SHORE: 3 . Ometers

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   149
                                                TOTAL NUMBER OF TAXA PRESENT    10
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     2
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     1
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     3_
                                                   NUMBER OF SUNFISH SPECIES     1
                                                 NUMBER OF HEADWATER SPECIES     2
                                                    NUMBER OF MINNOW SPECIES     4
                                         NUMBER OF ROUND BODY SUCKER SPECIES     (>
                                             NUMBER OF SUCKER FAMILY SPECIES     0
                                                 NUMBER OF SENSITIVE SPECIES     0
                                                  NUMBER OF TOLERANT SPECIES     £
                 PROPORTION OF  LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT    0.0%
                   PROPORTION OF  HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT    20.1%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    81.2%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT    19.5%
                                    PROPORTION  OF INSECTIVORES TO TOTAL COUNT    31.5%
                                      PROPORTION OF CARNIVORES TO TOTAL COUNT    0.0%
                                        PROPORTION OF  PIONEERS TO TOTAL COUNT    75.8%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    17.4%
                                                   PERCENT  D.E.L.T.  ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-KRIE LAKE PLAIN ECORBGION

                                          TPS SAMPLE #:TS91109    IDEM #:91109.0

3ITE:ROBINSON CRKXK            COUNTY:ALLKH        LOCATION:COVERDALE RD.  (U/S)               COLLECTION DATE:15-Jul-1991
riME:1430hr  LATITUDE/LONGITUDE :4£' 59." 40.00/85' .13" 33.0°      DRAINAGE -.T^laq. miles
3EGMENT:35-LITTLE WABASH RIVER                              NATURAL REGION CODE-.5C-BLUFFTON TILL PLAIN SECTION
rOPOMAP: GARRETT                     HYDROLOGIC UNIT:4100004-ST. MARY'S RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE:SOmeters
                             VOLTS: 300.  DC  CAPTURE DEPTH-.< 0.20meter3  DISTANCE TO  SHORE: 3 . Ometers

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    10
                                                TOTAL NUMBER OF TAXA PRESENT     3_
                                               NUMBER OF LARGE RIVER SPECIES     £
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     1
                                                   NUMBER OF SUNFISH SPECIES     1
                                                 NUMBER OF HEADWATER SPECIES     £
                                                    NUMBER OF MINNOW SPECIES     0
                                         NUMBER OF ROUND BODY SUCKER SPECIES     £
                                             NUMBER OF SUCKER FAMILY SPECIES     £
                                                 NUMBER OF SENSITIVE SPECIES     £
                                                  NUMBER OF TOLERANT SPECIES     1
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                      PROPORTION OF TOLERANTS TO TOTAL COUNT    30.0%
                                      PROPORTION OF OMNIVORES TO TOTAL COUNT     0.0%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    40.0%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT    60.0%
                                       PROPORTION OF PIONEERS TO TOTAL COUNT    40.0%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT     0.0%
                                                  PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                         INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-ERIK LAKE PLAIN ECOSSQION

                                          TPS  SAMPLE #:TS9iriO    IDEM 8:91110.0

SITE:LITTLK RXVKR              COUNTY:ALLXN        LOCATION:SMITH RD.  (U/S)                    COLLECTION DATE:15-Jul-19S
TIME:1507hr  LATITUDE/LONGITUDE :4_1'  1"  12.0°/85' 12" 29.0°      DRAINAGE: Z^Osq. miles
SEGMENT:21-ST. MARYS RIVER                                  NATURAL REGION CODE:SC-BLUFFTON TILL PLAIN SECTION
TOPOMAP: FORT WAYNE WEST             HYDROLOGIC UNIT:4100004-ST. MARY'S RIVER  BASIN (MAUMEE RIVER BASIN)

                             GEAR: LONGLINE      NET MESH: 0 . 318cm  SAMPLING DISTANCE :.50meters
                             VOLTS:300   DC   CAPTURE DEPTH:< 0.40meters  DISTANCE TO SHORE:2.Smeters

                                          TOTAL NUMBER OF INDIVIDUALS COUNTED     57
                                                TOTAL NUMBER OF TAXA PRESENT     10
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     0
                                                    NUMBER OF MADTOM SPECIES     1
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER  OF DARTER/MADTOM/SCULPIN SPECIES     1
                                                   NUMBER OF SUNFISH SPECIES     2
                                                 NUMBER OF HEADWATER SPECIES     0_
                                                    NUMBER OF MINNOW SPECIES     4
                                          NUMBER OF ROUND BODY SUCKER SPECIES     £
                                             NUMBER OF SUCKER FAMILY SPECIES     1
                                                 NUMBER OF SENSITIVE SPECIES     0
                                                  NUMBER OF TOLERANT SPECIES     6
                 PROPORTION  OF  LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     O.Ot
                   PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT     49.1%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT     13.3%
                                    PROPORTION OF  INSECTIVORES TO TOTAL COUNT     22.8%
                                      PROPORTION OF  CARNIVORES TO TOTAL COUNT     1.8%
                                        PROPORTION OF  PIONEERS TO TOTAL COUNT     71.9%

                               PROPORTION OF SIMPLE  LITHOPHILS TO TOTAL COUNT      1.8%
                                                   PERCENT  D.E.L.T.  ANOMOLIES      0.0%

-------
                                        INDIANA DEPARTMENT OP ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-KRIK LAJCB PLAIN BCORBGION

                                          TPS SAMPLE #:TS91111    IDEM »:91111.0

3ITE:OTUkHMI MCCULLOCH DITCH 1  COUNTY:ALLEH        LOCATION:SOUTH BBKD RD. (U/S)              COLLECTION DATE: 15-Jul-1991
riME:1536hr  LATITUDE/LONGITUDE:41'  3"  i^O'/SS1 13" 56.0°      DRAINAGE: 10.4sq. miles
SEGMENT:35-LITTLE HABASH RIVER                              NATURAL REGION CODE:5C-BLUFFTON TILL PLAIN  SECTION
rOPOMAP:  FORT WAYNE WEST             HYDROLOGIC UNIT:4100004-ST. MARY'S RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH: 0 . 318cm  SAMPLING DISTANCE: SOmetera
                             VOLTS:3JM)  DC  CAPTURE DEPTH:< 0.30meters  DISTANCE TO  SHORE:3.Smeters

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   293
                                                TOTAL NUMBER OF TAXA PRESENT    ^0
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     1
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     2
                                                   NUMBER OF SUNFISH SPECIES     0
                                                 NUMBER OF HEADWATER SPECIES     1
                                                    NUMBER OF MINNOW SPECIES     6
                                         NUMBER OF ROUND BODY SUCKER SPECIES     0
                                             NUMBER OF SUCKER FAMILY SPECIES     1
                                                 NUMBER OF SENSITIVE SPECIES     <)
                                                  NUMBER OF TOLERANT SPECIES     6_
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT    17.1%

                                      PROPORTION OF TOLERANTS TO TOTAL COUNT    44.7%
                                      PROPORTION OF OMNIVORES TO TOTAL COUNT     6.5%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    18.1%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT     0.0%
                                       PROPORTION OF PIONEERS TO TOTAL COUNT    61.8%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    37.5%
                                                  PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-ZRIK LAKK PLAIN ICOREGION

                                          TPS SAMPLE #:TS91112

SITE:JOHU DIKHL DITCH          COUNTY:DRKXLB       LOCATION:S.R. 327  (U/S)                     COLLECTION DATE:16-Jul-199
TIME:840hr   LATITUDE/LONGITUDE: 41' 23" 17.0°/8S'  8" 11.0°      DRAINAGE: T^sq. miles
SEGMENT-.18-CEDAR CREEK                                      NATURAL REGION CODE:4-NORTHERN  LAKES NATURAL REGION
TOPOMAP-. CORUNNA.                     HYDROLOGIC ONIT:4100003-ST. JOSEPH RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR: LONGLINE      NET MESH: 0. 318cm  SAMPLING DI STANCE -.SOmeters
                             VOLTS :30£  DC  CAPTURE DEPTH :< 0.6 Procters  DISTANCE TO SHORE: 3 . Imeters

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   133
                                                TOTAL NUMBER OF TAXA PRESENT    14
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     1
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     2
                                                   NUMBER OF SUNFISH SPECIES     2.
                                                 NUMBER OF HEADWATER SPECIES     2
                                                    NUMBER OF MINNOW SPECIES     5
                                         NUMBER OF ROUND BODY SUCKER SPECIES     (>
                                             NUMBER OF  SUCKER FAMILY SPECIES     1
                                                 NUMBER OF  SENSITIVE SPECIES     0
                                                  NUMBER OF TOLERANT SPECIES     7
                 PROPORTION OF  LARGE RIVER  SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                   PROPORTION OF HEADWATER  SPECIES INDIVIDUALS TO TOTAL COUNT    24.8*

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    56.4%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT    20.3%
                                    PROPORTION  OF INSECTIVORES TO TOTAL COUNT    48.9%
                                      PROPORTION OF CARNIVORES TO TOTAL COUNT     2.3%
                                        PROPORTION OF  PIONEERS TO TOTAL COUNT    40.6%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    28.6%
                                                   PERCENT D.E.L.T.  ANOMOLIES      0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HDRON-KRIK LAKH PUO2J BCORBGION

                                          TPS SAMPLE #:TS91113    IDEM #:91113.0

3ITE:OBKR DITCH                COUNTY:DEKALB       LOCATIONS.R. 19  (U/S) BRIDGE               COLLECTION DATE:16-Jul-1991
[•IME:j>12hr   LATITUDE/LONGITUDE :4_1' 2_3" 44.0"/85'  6." 52.0"      DRAINAGE:T^±aq. miles
SEGMENT: 18-CEDAR CREEK                                      NATURAL REGION CODE:4-NORTHERN LAKES NATURAL REGION
rOPOMAP:  WATERLOO                    HYDROLOGIC ONIT:4100003-ST. JOSEPH RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH: 0 .318cm  SAMPLING DISTANCE:BOmeters
                             VOLTS:300  DC  CAPTURE DEPTH:< 0.40meter3  DISTANCE TO  SHORE:2.3meter3

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    6£
                                                TOTAL NUMBER OF TAXA PRESENT    11
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     1
                                                   NUMBER OF SUNFISH SPECIES     3_
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     5
                                         NUMBER OF ROUND BODY SUCKER SPECIES     0
                                             NUMBER OF SUCKER FAMILY SPECIES     1
                                                 NUMBER OF SENSITIVE SPECIES     1
                                                  NUMBER OF TOLERANT SPECIES     J5
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                      PROPORTION OF TOLERANTS TO TOTAL COUNT    60.3%
                                      PROPORTION OF OMNIVORES TO TOTAL COUNT     5.9%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    42.6%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT     4.4%
                                       PROPORTION OF PIONEERS TO TOTAL COUNT    66.2%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    20.6%
                                                  PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-BRIX LAKE PLAIN BCORBGION

                                          TPS SAMPLE tt:TS91114

SITE:CBDAR CRKHK               COUNTY:DKK»LB       LOCATIONS.R. 35 (D/S)                      COLLECTION DATE :16-Jul-199
TIME:.954hr   LATITUDE/LONGITUDE: 41' 23."  5^0°/85'  1" 24.0°      DRAINAGE: 74 .4sq. miles
SEGMENT: 18-CEDAR CREEK                                      NATURAL REGION CODE:SC-BLUFFTON TILL PLAIN SECTION
TOPOMAP: WATERLOO                    HYDROLOGIC UNIT:4100003-ST. JOSEPH RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH: 0.318cm  SAMPLING DISTANCE:IQOmetera
                             VOLTS-.300.  DC  CAPTURE DEPTH:< O.SOmeters  DISTANCE TO SHORE:6.Ometers

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   187
                                                TOTAL NUMBER OF TAXA PRESENT    15
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     1
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     2
                                                   NUMBER OF SUNFISH SPECIES     2
                                                 NUMBER OF HEADWATER SPECIES     3
                                                    NUMBER OF MINNOW SPECIES     7
                                         NUMBER OF ROUND BODY SUCKER SPECIES     0
                                             NUMBER OF  SUCKER FAMILY SPECIES     1
                                                 NUMBER OF SENSITIVE SPECIES     1
                                                  NUMBER OF  TOLERANT SPECIES     a
                PROPORTION OF  LARGE RIVER  SPECIES INDIVIDUALS TO  TOTAL COUNT    0.0%
                   PROPORTION OF HEADWATER  SPECIES INDIVIDUALS TO  TOTAL COUNT    5.9%

                                       PROPORTION OF  TOLERANTS TO  TOTAL COUNT    77.0%
                                       PROPORTION OF  OMNIVORES TO  TOTAL COUNT    8.0%
                                    PROPORTION OF INSECTIVORES TO  TOTAL COUNT    31.0%
                                      PROPORTION OF CARNIVORES TO  TOTAL COUNT    0.5%
                                        PROPORTION OF PIONEERS TO  TOTAL COUNT    77.5%

                               PROPORTION OF SIMPLE  LITHOPHILS TO  TOTAL COUNT     E.0%
                                                   PERCENT D.E.L.T.  ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               BORON-ERIK LUCK PLAIN ECORBGION

                                          TPS SAMPLE #:TS91115    IDEM #:91115.0

iITE:CKD*R CREEK               COUNTY:DEKALB       LOCATIONS.R. 8  (U/S)                      COLLECTION DATE:16-Jul-1991
'IME:1047hr  LATITUDE/LONGITUDE:41.' 22"  2J±°/8S'  3"  3.0°      DRAINAGE:87.3sa. miles
SEGMENT: IB-CEDAR CREEK                                      NATURAL REGION CODE: 5C-BLUFFTON TILL PLAIN SECTION
•OPOMAP:  AUBURN                      HYDROLOGIC UNIT:4100003-ST. JOSEPH RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0. 318cm  SAMPLING DISTANCE: IQOmeters
                             VOLTS:300.  DC  CAPTURE DEPTH:< 0.30meters  DISTANCE TO  SHORE-.6 .Ometers

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   105
                                                TOTAL NUMBER OF TAXA PRESENT    12
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     2
                                                    NUMBER OF MADTOM SPECIES     £
                                                   NUMBER OF SCULPIN SPECIES     1
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     3_
                                                   NUMBER OF SUNFISH SPECIES     2.
                                                 NUMBER OF HEADWATER SPECIES     2.
                                                    NUMBER OF MINNOW SPECIES     3
                                         NUMBER OF ROUND BODY SUCKER SPECIES     2
                                             NUMBER OF SUCKER FAMILY SPECIES     1
                                                 NUMBER OF SENSITIVE SPECIES     1
                                                  NUMBER OF TOLERANT SPECIES     6
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT    20.0%

                                      PROPORTION OF TOLERANTS TO TOTAL COUNT    88.6%
                                      PROPORTION OF OMNIVORES TO TOTAL COUNT     8.6%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    53.3%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT     2.9%
                                       PROPORTION OF PIONEERS TO TOTAL COUNT    65.7%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    20.0%
                                                  PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HDRON-BRIB LXKX PLAIN BCORBOIOM

                                          TPS SAMPLE tt:TS91116    IDEM #:91116.0

SITE:JOHN D1EHL DITCH          COUNTY:DKKALB       LOCATION:A.UBORN DR. (C.R. 48)  (D/S)         COLLECTION DATE: 16-Jul-199
TIME:1127hr  LATITUDE/LONGITUDE:4i' 2_1"  7_._0° /SS_'  4" 48.0"      DRAINAGE: 37.4sq. miles
SEGMENT: 18-CEDAR CREEK                                      NATURAL REGION CODE:SC-BLUFFTON TILL PLAIN SECTION
TOPOMAP: AUBURN                      HYDROLOGIC UNIT:4100003-ST. JOSEPH RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE:lOOmeters
                             VOLTS :.300  DC  CAPTURE DEPTH :< 0.70meter3  DISTANCE  TO SHORE:6.Ometera

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    155
                                                TOTAL NUMBER OF TAXA PRESENT    12
                                               NUMBER OF LARGE RIVER SPECIES      0
                                                    NUMBER OF DARTER SPECIES      3.
                                                    NUMBER OF MADTOM SPECIES      0
                                                   NUMBER OF SCULPIN SPECIES      1
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES      3
                                                   NUMBER OF SUNFISH SPECIES      1
                                                 NUMBER OF HEADWATER SPECIES      3_
                                                    NUMBER OF MINNOW SPECIES      6
                                         NUMBER OF ROUND BODY SUCKER SPECIES      £
                                             NUMBER OF SUCKER FAMILY SPECIES      1
                                                 NUMBER OF  SENSITIVE SPECIES      1
                                                  NUMBER OF TOLERANT SPECIES      4
                PROPORTION OF  LARGE RIVER  SPECIES INDIVIDUALS TO  TOTAL COUNT      0.0%
                   PROPORTION OF  HEADWATER  SPECIES INDIVIDUALS TO  TOTAL COUNT     71.6%

                                       PROPORTION OF TOLERANTS TO  TOTAL COUNT     54.8%
                                       PROPORTION OF OMNIVORES TO  TOTAL COUNT      3.9%
                                    PROPORTION OF  INSECTIVORES To  TOTAL COUNT     42.6%
                                      PROPORTION OF  CARNIVORES TO  TOTAL COUNT      0.6% .
                                        PROPORTION OF  PIONEERS TO  TOTAL COUNT     19.4%

                               PROPORTION OF SIMPLE  LITHOPHILS TO  TOTAL COUNT     45.8%
                                                   PERCENT D.E.L.T.  ANOMOLIES      0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HORON-BRIE LAKE PLAIN KCOREOION

                                          TPS SAMPLE #:TS91117

3ITE.-CSDAR CREEK               COUNTY:DBKALB       LOCATION:S.R. 427 (D/S)                    COLLECTION DATE:16-Jul-1991
CIME:1407hr  LATITUDE/LONGITUDE:^1 19" 17.0°/85'  4" 39.0°      DRAINAGE: 133 .6sq. miles
SEGMENT: 18-CEDAR CREEK                                      NATURAL REGION CODE:5C-BLUFFTOS TILL PLAIN SECTION
rOPOMAP:  AUBURN                      HYDROLOGIC UNIT:4100003-ST. JOSEPH RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH: 0 .318cm  SAMPLING DISTANCE: IQOmeters
                             VOLTS:300  DC  CAPTURE DEPTH:.: l.OOmetera  DISTANCE TO  SHORE:6 .4meters

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   102
                                                TOTAL NUMBER OF TAXA PRESENT    14
                                               NUMBER OF LARGE RIVER SPECIES     0_
                                                    NUMBER OF DARTER SPECIES     3_
                                                    NUMBER OF MAOTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     1
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     4
                                                   NUMBER OF SUNFISH SPECIES     2
                                                 NUMBER OF HEADWATER SPECIES     3_
                                                    NUMBER OF MINNOW SPECIES     5
                                         NUMBER OF ROUND BODY SUCKER SPECIES     1
                                             NUMBER OF SUCKER FAMILY SPECIES     2.
                                                 NUMBER OF SENSITIVE SPECIES     1
                                                  NUMBER OF TOLERANT SPECIES     t
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     4.9%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    84.3%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT    17.6%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    44.1%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT     0.0%
                                       PROPORTION OF PIONEERS TO TOTAL COUNT    76.5%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    13.7%
                                                  PERCENT  D.E.L.T.  ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-ERIK LAKB PLAIN BCORKGION

                                          TPS SAMPLE #:TS91118    IDEM #:91118.0

SITE:LITTLK CEDAR CKXXK        COUNTY:DKKALB       LOCATION:C.R. 64  (D/S)                      COLLECTION DATE:16-Jul-199
TIME:1522hr  LATITUDE/LONGITUDE: 41' 17" 37.0°/B5'  8" 26.0°      DRAINAGE:45.Bag. miles
SEGMENT: 18-CEDAR CREEK                                      NATURAL REGION CODE:5C-BLDFFTON TILL PLAIN SECTION
TOPOMAP: GARRETT                     HYDROLOGIC ONIT-.41.00003-ST. JOSEPH RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH; 0 . 318cm  SAMPLING DISTANCE: IQOmetera
                             VOLTS-.3CH3  DC  CAPTURE DEPTH:< O.SOmeters  DISTANCE TO  SHORE: 8 . Smetera

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    115
                                                TOTAL NUMBER OF TAXA PRESENT    16
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     4
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     1
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     5_
                                                   NUMBER OF SUNFISH SPECIES     1
                                                 NUMBER OF HEADWATER SPECIES     2
                                                    NUMBER OF MINNOW SPECIES     6
                                         NUMBER OF ROUND BODY SUCKER SPECIES     1
                                             NUMBER OF SUCKER FAMILY SPECIES     2
                                                 NUMBER OF SENSITIVE SPECIES     3_
                                                  NUMBER OF TOLERANT SPECIES     B
                PROPORTION OF  LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     9.6%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT     53.0%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT     27.0%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT     35.7%
                                      PROPORTION OF CARNIVORES TO TOTAL COUNT     1.7%
                                        PROPORTION OF  PIONEERS TO TOTAL COUNT     41.7%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT     29.6%
                                                   PERCENT  D.E.L.T.  ANOMOLIES      0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               BORON-BRIE LAKE PLAIN BCOREGION

                                          TPS SAMPLE #:TS91119    IDEM #:91119.0

;iTE:CBDAR CREEK                COUNTY: DEKALB       LOCATION: C. R.  68 (D/S)                      COLLECTION DATE: 16-Jul-1991
'1MB: 144 Shr  LATITUDE/LONGITUDE:^'  16" tT-O'/BS'  6_" 38.0"      DRAINAGE: 136 . 8sq. miles
iEGMENT: 18-CEDAR  CREEK                                      NATURAL REGION CODE: 5C-BLUFFTON TILL PLAIN SECTION
•OPOMAP: AUBURN                       HYDROLOGIC UNIT:4100003-ST.  JOSEPH RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH: 0 .318cm  SAMPLING DISTANCE: IQOmeters
                             VOLTS :3JH>  DC  CAPTURE DEPTH:< l.OOmeters  DISTANCE TO SHORE:6 . Ometers

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    92
                                                TOTAL NUMBER OF TAXA PRESENT    11
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     £
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     1
                                                   NUMBER OF SUNFISH SPECIES     2
                                                 NUMBER OF HEADWATER SPECIES     1
                                                    NUMBER OF MINNOW SPECIES     6
                                         NUMBER OF ROUND BODY SUCKER SPECIES     I
                                             NUMBER OF SUCKER FAMILY SPECIES     2
                                                 NUMBER OF SENSITIVE SPECIES     1
                                                  NUMBER OF TOLERANT SPECIES     7.
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL CO0NT     0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT    14.1%

                                      PROPORTION OF TOLERANTS TO TOTAL COUNT    79.3%
                                      PROPORTION OF OMNIVORES TO TOTAL COUNT    17.4%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    59.8%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT     0.0%
                                       PROPORTION OF PIONEERS TO TOTAL COUNT    66.3%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    22.8%
                                                  PERCENT D.E.L.T. ANOMOLIES      0.0%

-------
                                         INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                              FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HOROH-KRXB LAKE PLAIN KCOREQION

                                          TPS SAMPLE #:TS91120

SITE:ST. JOSEPH RTVKR          COUNTY-.DBKALB       LOCATIONS.R. 8  (D/S)                       COLLECTION DATB:17-Jul-199
TIME:1040hr  LATITUDE/LONGITUDE:41' 20"  51. 0°/_84' SO.' 40.0°      DRAINAGE:641.Oaq. miles
SEGMENT:20-ST. JOSEPH RIVER                                 NATURAL REGION CODE:SC-BLUFFTON TILL PLAIN SECTION"
TOPOMAP: HICKSVILLE                  HYDROLOGIC UNIT:4100003-ST. JOSEPH RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR;SPORT  YAK II  NET MESH:0 . 318cm  SAMPLING DISTANCE:300meter3
                             VOLTS :3QO_   DC  CAPTURE DEPTH:< 1.20meCers  DISTANCE TO  SHORE:41 .Smeters

                                          TOTAL NUMBER OF INDIVIDUALS COUNTED   293
                                                TOTAL NUMBER OF TAXA PRESENT     18
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     
-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-ERIK LAKE PLAIN KCORW3ION

                                          TPS SAMPLE #:TS91121    IDEM #:91121.0

iITE:ST. JOSEPH RIVER          COUNTY:DEKALB       LOCATION:C.R.  64 (U/S)                      COLLECTION DATE :17-Jul-1991
'IME:1420hr  LATITUDE/LONGITUDE: 41'  17" 54.0°/84' £3" 58.0"      DRAINAGE: 703 .Sag, miles
:EGMENT:20-ST. JOSEPH RIVER                                 NATURAL REGION CODE: 5C-BLUFFTON TILL PLAIN SECTION
'OPOMAP: SAINT JOE                    HYDROLOGIC UNIT:4100003-ST.  JOSEPH RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:SPORT YAK II  NET MESH: 0 . 318cm  SAMPLING DISTANCE:300meters
                             VOLTS: 3JH)   DC  CAPTURE DEPTH :< 1.20meter3  DISTANCE TO SHORE: 22 . Ometers

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   339
                                                TOTAL NUMBER OF TAXA PRESENT    23.
                                               NUMBER OF LARGE RIVER SPECIES     2
                                                    NUMBER OF DARTER SPECIES     3.
                                                    NUMBER OF MADTOM SPECIES     1
                                                   NUMBER OF  SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     4
                                                   NUMBER OF  SUNFISH SPECIES     3_
                                                 NUMBER OF HEADWATER SPECIES     2
                                                    NUMBER OF MINNOW SPECIES     9_
                                         NUMBER OF ROUND BODY SUCKER SPECIES     4
                                             NUMBER OF SUCKER FAMILY SPECIES     4
                                                 NUMBER OF SENSITIVE SPECIES     £
                                                  NUMBER OF TOLERANT SPECIES     7
               PROPORTION  OF LARGE RIVER SPECIES INDIVIDUALS TO  TOTAL COUNT     3.5%
                 PROPORTION  OF HEADWATER SPECIES INDIVIDUALS TO  TOTAL COUNT    10.0%

                                     PROPORTION OF TOLERANTS TO  TOTAL COUNT    50.7%
                                     PROPORTION OF OMNIVORES TO  TOTAL COUNT    23.9%
                                  PROPORTION OF INSECTIVORES TO  TOTAL COUNT    68.7%
                                     PROPORTION OF CARNIVORES TO  TOTAL COUNT     5.3%
                                       PROPORTION OF PIONEERS TO  TOTAL COUNT    40.4%

                             PROPORTION OF SIMPLE LITHOPHILS TO  TOTAL COUNT    14.5%
                                                  PERCENT  D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                              FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HORON-SR1B UUKB PLAIN KCORBGION

                                          TPS SAMPLE #:TS91122    IDEM  #:91122.0

SITE:ST. MARY'S RTVXR          COUNTY:ADAMS         LOCATIONS.R. 101  (tJ/S)                     COLLECTION DATE:18-Jul-199
TIME:915hr   LATITUDE/LONGITUDE.-40' 46" 4S.O-'/84' SO"  31.0"      DRAINAGE:550.Oaq.  miles
SEGMENT:21-ST. MARYS RIVER                                  NATURAL REGION CODE:5C-BLUFFTON TILL PLAIN SECTION
TOPOMAP: WREN                        HYDROLOGIC ONIT:4100004-ST. MARY'S RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:SPORT YAK  II  NET MESH: 0 . 318cm  SAMPLING DISTANCE: 300meters
                             VOLTS: 3 00  DC   CAPTURE DEPTH:< 1.20meters  DISTANCE TO SHORE: 19 . Ometers

                                          TOTAL NUMBER  OF INDIVIDUALS COUNTED    188
                                                TOTAL  NUMBER OF TAXA PRESENT     23.
                                               NUMBER  OF LARGE RIVER SPECIES      4
                                                    NUMBER OF DARTER SPECIES      2
                                                    NUMBER OF MADTOM SPECIES      2
                                                    NUMBER OF SCULPIN SPECIES      Q.
                                      NUMBER  OF DARTER/MADTOM/SCULPIN SPECIES      4
                                                    NUMBER OF SUNFISH SPECIES      4
                                                 NUMBER OF HEADWATER SPECIES      0
                                                    NUMBER OF MINNOW SPECIES      £
                                          NUMBER OF  ROUND BODY SUCKER SPECIES      0
                                              NUMBER OF SUCKER FAMILY SPECIES      2
                                                 NUMBER OF SENSITIVE SPECIES      3
                                                  NUMBER OF TOLERANT SPECIES     10
                 PROPORTION OF LARGE  RIVER SPECIES  INDIVIDUALS TO  TOTAL COUNT     10.1%
                   PROPORTION OF HEADWATER SPECIES  INDIVIDUALS TO  TOTAL COUNT      0.0%

                                       PROPORTION  OF TOLERANTS TO  -TOTAL COUNT     62.2%
                                       PROPORTION  OF OMNIVORBS TO  TOTAL COUNT     36.7%
                                    PROPORTION OF  INSECTIVORBS TO  TOTAL COUNT     52.1%
                                      PROPORTION OF CARNIVORES TO  TOTAL COUNT     10.1%
                                        PROPORTION OF  PIONEERS TO TOTAL COUNT     17.0%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    29.3%
                                                   PERCENT D.E.L.T.  ANOMOLIES      0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-ERIK LAKE PLAIN ECORBGION

                                          TPS SAMPLE #:TS91123    IDEM ft:91123.0

3ITE:HOLTHOUSB DITCH           COUNTY:ADAMS        LOCATIONS.R. 224  (U/S)                    COLLECTION DATE: 18-Jul-1991
riME:1140hr  LATITUDE/LONGITUDE :4CP 4£" 56.0"/84' 57" 22.0°      DRAINAGE: 32. Sag. miles
SEGMENT: 21-ST. MARYS RIVER                                  NATURAL REGION CODE : 5C-BLUFFTON TILL  PLAIN SECTION
TOPOMAP:  DECATOR                     HYDROLOGIC UNIT:4100004-ST. MARY'S RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE:5£meters
                             VOLTS.-302  DC  CAPTURE DEPTH :< 0.20meter3  DISTANCE TO  SHORE:3.Smeters

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    £9
                                                TOTAL NUMBER OF TAXA PRESENT     9
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     £
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     1
                                                   NUMBER OF SUNFISH SPECIES     2
                                                 NUMBER OF HEADWATER SPECIES     <>
                                                    NUMBER OF MINNOW SPECIES     4
                                         NUMBER OF ROUND BODY SUCKER SPECIES     0_
                                             NUMBER OF SUCKER FAMILY SPECIES     1
                                                 NUMBER OF SENSITIVE SPECIES     0
                                                  NUMBER OF TOLERANT SPECIES     5
                PROPORTION OF  LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT     85.5%
                                       PROPORTION OF OMNIVORES TO TOTAL COUNT     26.1%
                                   PROPORTION  OF INSECTIVORES TO TOTAL COUNT     36.3%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT     5.B%
                                        PROPORTION OF  PIONEERS TO TOTAL COUNT    87.0%

                               PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    4.3%
                                                  PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                         INDIANA DEPARTMENT  OF  ENVIRONMENTAL MANAGEMENT
                                                   OFFICE  OF WATER MANAGEMENT
                                                   BIOLOGICAL STUDIES  SECTION

                                              FISH  COMMUNITY IBI  DEVELOPMENT DATA
                                               HURON-ERIB LAKE PLAIN  BCORBOION

                                          TPS SAMPLE #;TS91124    IDEM #:91124.0

SITE.-OKWa DITCH               COUNTY:ADAMS        LOCATION: 100K (0/S)                         COLLKCTION DATE:18-Jul-199
TIME:1324hr  LATITUDE/LONGITUDE:^' 52"  27.0°/84-  55" 12.0"       DRAINAGE: 10.lag.  miles
SEGMENT:21-ST. MARYS RIVER                                  NATURAL REGION  CODE:5C-BLUFFTON TILL PLAIN SECTION
TOPOMAP: DECATUR                     HYDROLOGIC UNIT:4100004-ST.  MARY'S RIVER  BASIN (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0. 318cm  SAMPLING  DISTANCE:5_0meters
                             VOLTS: 300   DC  CAPTURE DEPTH :< O.SOmeters  DISTANCE TO SHORE :4 . Ometers

                                         TOTAL NUMBER OF  INDIVIDUALS  COUNTED  156
                                                TOTAL NUMBER OF  TAXA  PRESENT    8.
                                               NUMBER OF  LARGE RIVER  SPECIES    0
                                                    NUMBER  OF  DARTER  SPECIES    2
                                                    NUMBER  OF  MADTOM  SPECIES    0
                                                   NDMBBR OF SCULPIN  SPECIES    £
                                     NUMBER OF DARTER/MADTOM/SCULPIN  SPECIES    2
                                                   NUMBER OF SUNFISH  SPECIES    0
                                                 NUMBER OF  HEADWATER  SPECIES    0
                                                    NUMBER  OF  MINNOW  SPECIES    4
                                         NUMBER OF ROUND  BODY  SUCKER  SPECIES    <>
                                              NUMBER OF SUCKER  FAMILY  SPECIES    1
                                                 NUMBER OF  SENSITIVE  SPECIES    0
                                                   NUMBER  OF TOLERANT  SPECIES    4
                PROPORTION OF LARGE RIVER SPECIES  INDIVIDUALS  TO TOTAL COUNT    0.0%
                  PROPORTION OF HEADWATER SPECIES  INDIVIDUALS  TO TOTAL COUNT    0.0%

                                      PROPORTION OF TOLERANTS  TO TOTAL COUNT    47.4%
                                      PROPORTION OF OMNIVORES  TO TOTAL COUNT    18.6%
                                   PROPORTION OF INSECTIVORES  TO ""VTAL COUNT    31.3%
                                     PROPORTION OF CARNIVORES  TO TOTAL COUNT    0.6%
                                        PROPORTION  OF PIONEERS  TO TOTAL COUNT    89.7%

                              PROPORTION OF SIMPLE LITHOPHILS  TO TOTAL COUNT    21.8%
                                                   PERCENT D.E.L.T. ANOMOLIES    0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES  SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HORON-ERIK LAKE PLAIN  BCOREGION

                                          TPS SAMPLE #:TS91125

3ITE:DNTRIB.  PTATROCK CRBBK    CODNTY:ADAMS        LOCATION:C.R. 1100N (0/S)                   COLLECTION DATE: 18-Jul-1991
riMB:1400hr  LATITUDE/LONGITUDE:40; 54" 26.0°/84' 4£" 44.0°      DRAINAGE:28.Osq. miles
SEGMENT:21-ST.  MARYS RIVER                                  NATURAL REGION CODE:6-BLACK  SWAMP NATURAL REGION
rOPOMAP:  DIXON                       HYDROLOGIC UNIT:4100007-PLATROCK CREEK BASIN  (MADMEE  RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE:SOmeters
                             VOLTS :3_0£  DC  CAPTURE DEPTH:.: 0.30meter3  DISTANCE TO SHORE: 3 . Ometers

                                         TOTAL NUMBER OF INDIVIDUALS  COUNTED     99
                                                TOTAL NUMBER OF TAXA  PRESENT     10
                                               NUMBER OF LARGE RIVER  SPECIES     0_
                                                    NUMBER OF DARTER  SPECIES      1
                                                    NUMBER OF MADTOM  SPECIES     (>
                                                   NUMBER OF SCULPIN  SPECIES      <>
                                     NUMBER OF DARTER/MADTOM/SCULPIN  SPECIES      1
                                                   NUMBER OF SUNFTSH  SPECIES      1
                                                 NUMBER OF HEADWATER  SPECIES      0
                                                    NUMBER OF MINNOW  SPECIES      5
                                         NUMBER OF ROUND BODY SUCKER  SPECIES      0
                                             NUMBER OF  SUCKER FAMILY  SPECIES      1
                                                 NUMBER OF SENSITIVE  SPECIES      0
                                                  NUMBER OF TOLERANT  SPECIES      6.
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT      0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT      0.0%

                                      PROPORTION OF TOLERANTS TO TOTAL COUNT     92.9%
                                      PROPORTION OF OMNIVORES TO TOTAL COUNT     10.1%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT     56.6%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT      0.0%
                                        PROPORTION OF  PIONEERS TO TOTAL COUNT     39.4%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT      7.1%
                                                  PERCENT D.E.L.T.  ANOMOLIES      0.0%

-------
                                        INDIAHA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HORON-KR1B LAKE PLAIN BCORKOION

                                          TPS SAMPLE #:TS91126    IDKM #:91126.0

SITE:NIOCKLSKH CKBBK           COUNTY: ADAMS        LOCATIONS.R. 550* (n/S)                    COLLECTION DATE: 18-Jul-199:
TIME:1455hr  LATITUDE/LONGITUDE:4£' 53." 32.0°/85'  2" 39.0°      DRAINAGE: 11.7sq. miles
SEGMENT:21-ST. MARYS RIVER                                  NATURAL REGION CODE:5C-BLUFFTON TILL PLAIN SECTION
TOPOMAP: POE                         HYDROLOGIC UHIT:4100004-ST. MARY'S RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH: 0 . 318cm  SAMPLING DISTANCE:BOmeters
                             VOLTS: 300  DC  CAPTURE DEPTH :< 0.30meters  DISTANCE TO  SHORE: 3 . Smeters

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   211
                                                TOTAL NUMBER OF TAXA PRESENT    13_
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     1
                                                   NUMBER OF SUNFISH SPECIES     2_
                                                 NUMBER OP HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     7
                                         NUMBER OF ROUND BODY SUCKER SPECIES     0
                                             NUMBER OF SUCKER FAMILY SPECIES     1
                                                 NUMBER OF SENSITIVE SPECIES     0
                                                  NUMBER OF TOLERANT SPECIES     £
                 PROPORTION  OF  LARGE RIVER  SPECIES  INDIVIDUALS TO  TOTAL  COUNT     0.0%
                   PROPORTION OF  HEADWATER  SPECIES  INDIVIDUALS TO  TOTAL  COUNT     0.0*

                                       PROPORTION OF TOLERANTS TO  TOTAL  COUNT     62.9%
                                       PROPORTION OF OMNIVORES TO  TOTAL  COUNT     51.7%
                                    PROPORTION  OF INSECTIVORES TO  TOTAL  COUNT     35.1%
                                      PROPORTION OF CARNIVORES TO  TOTAL  COUNT     0.5%
                                        PROPORTION  OF  PIONEERS TO  TOTAL  COUNT     71.1%

                               PROPORTION OF SIMPLE LITHOPHILS TO  TOTAL  COUNT      3.3%
                                                   PERCENT D.E.L.T.  ANOMOLIES      0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HDRON-BR1B LAKE PLAIN KCORKGION

                                          TPS SAMPLE #:TS91127    IDEM tf:91127.0

;iTE:WinilEE RTVXR              COUNTY:ALLMJ        LOCATION:S.R. 24 (AT JOYCKKS PARK)         COLLECTION DATE:19-Jul-1991
:iME:902hr   LATITUDE/LONGITUDE: 4.1'   5*  ±^0."/SB'  1" 11.0°      DRAINAGE :«**.«SQ. miles
SEGMENT:19-MAUMBE RIVER (MAIN STEM)                          NATURAL REGION CODE:5C-BLUFFTON TILL PLAIN SECTION
•OPOMAP:  FORT WAYNE EAST             HYDROLOGIC UNIT:4100005-MAUMBE RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:SPORT YAK II  NET MESH:0.318cm  SAMPLING DISTANCE:400meters
                             VOLTS:3_00  DC  CAPTURE DEPTH:< 1.20meters  DISTANCE TO SHORE:34.Smetera

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   416
                                                TOTAL NUMBER OF TAXA PRESENT    3_1
                                               NUMBER OF LARGE RIVER SPECIES     3
                                                    NUMBER OF DARTER SPECIES     4
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     4
                                                   NUMBER OF SUNFISH SPECIES     7
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     £
                                         NUMBER OF ROUND BODY SUCKER SPECIES     4
                                             NUMBER OF SUCKER FAMILY SPECIES     5
                                                 NUMBER OF SENSITIVE SPECIES    10
                                                  NUMBER OF TOLERANT SPECIES     £
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT    10.3%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                      PROPORTION OF TOLERANTS TO TOTAL COUNT    41.6%
                                      PROPORTION OF OMNIVORES TO TOTAL COUNT    25.0%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    64.2%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT    10,6%
                                       PROPORTION OF PIONEERS TO TOTAL COUNT    30.5%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    19.7%
                                                  PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                         INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                              FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON-ERIE UKK PLAIN ECORBOION

                                          TPS SAMPLE #:TS91128

SITE:CEDAR CREEK               COUNTY:MiLKN        LOCATION:TONXKL RD.  (U/S 6 D/S)             COLLECTION DATE:22-Jul-199
TIME:1341hr  LATITUDE/LONGITUDE: 41' 13"  11.0°/85'  4" 32.0°      DRAINAGE: 27 O.Osg. miles
SEGMENT: 18-CEDAR CREEK                                      NATURAL REGION CODE:5C-BLOFFTON TILL  PLAIN SECTION
TOPOMAP: CEDARVILLE                  HYDROLOGIC UNIT:4100003-ST. JOSEPH RIVER BASIN  (MAUMEE RIVER BASIN)

                             GRAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE:200metera
                             VOLTS :3_0£   DC  CAPTURE DEPTH :< O.SOmeters  DISTANCE TO  SHORE:23.Bmetera

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    73
                                                TOTAL NUMBER OF TAXA PRESENT    IS
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     5
                                                    NUMBER OF MADTOM SPECIES     £
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     5
                                                   NUMBER OF SUNFISH SPECIES     2
                                                 NUMBER OF HEADWATER SPECIES     1
                                                    NUMBER OF MINNOW SPECIES     S
                                         NUMBER OF ROUND BODY SUCKER SPECIES     3.
                                              NUMBER OF SUCKER FAMILY SPECIES     3_
                                                 NUMBER OF SENSITIVE SPECIES     7
                                                  NUMBER OF TOLERANT SPECIES     3
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     1.4%

                                      PROPORTION OF TOLERANTS TO TOTAL COUNT    43.8%
                                      PROPORTION OF OMNIVORES TO TOTAL COUNT     8.2%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    SI.8%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT     0.0%
                                       PROPORTION OF PIONEERS TO TOTAL COUNT    37.0%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    28.8%
                                                  PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               BORON-KRIS LAKE PLAIN BCOREGION

                                                                  IDEM tt:91129.0

!ITE:ST.  JOSEPH RIVER          COUNTY:ALLEN        LOCATION:KMN BD. (D/S)                    COLLECTION DATE:22-Jul-1991
•IMErlSOOhr  LATITUDE/LONGITUDE: 41.'  li" 51.0°/85'  1" 30._00      DRAINAGE: 763 .Ssq. miles
IEGMENT:20-ST.  JOSEPH RIVER                                 NATURAL REGION CODE:5C-BLUFFTON TILL PLAIN SECTION
•OPOMAP:  CEDARVILLE                  HYDROLOGIC UNIT:4100003-ST. JOSEPH RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:SPORT YAK II  NET MESH:0 . 318cm  SAMPLING DISTANCE:4OOmeters
                             VOLTS -.300  DC  CAPTURE DEPTH:< 1.20meters  DISTANCE TO SHORE:27 . Ometers

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   489
                                                TOTAL NUMBER OF TAXA PRESENT    22
                                               NUMBER OF LARGE RIVER SPECIES     2
                                                    NUMBER OF DARTER SPECIES     5
                                                    NUMBER OF MADTOM SPECIES     £
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     5
                                                   NUMBER OF StJNFISH SPECIES     3
                                                 NUMBER OF HEADWATER SPECIES     1
                                                    NUMBER OF MINNOW SPECIES     6
                                         NUMBER OF ROUND BODY SUCKER SPECIES     5
                                             NUMBER OF SUCKER FAMILY SPECIES     5
                                                 NUMBER OF SENSITIVE SPECIES     8
                                                  NUMBER OF TOLERANT SPECIES     £
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     4.7%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.2%

                                      PROPORTION OF TOLERANTS TO TOTAL COUNT    81.0%
                                      PROPORTION OF OMNIVORES TO TOTAL COUNT    61.1%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    31.5%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT     7.0%
                                       PROPORTION OF PIONEERS TO TOTAL COUNT    17.4%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    11.5%
                                                  PERCENT D.E.L.T.  ANOMOLIES      0.0%

-------
                                         INDIANA DEPARTMENT OF  ENVIRONMENTAL MANAGEMENT
                                                   OFFICE  OF WATER MANAGEMENT
                                                   BIOLOGICAL STUDIES  SECTION

                                              FISH  COMMUNITY IBI  DEVELOPMENT DATA
                                               HURON-KRIS LAKE PLAIN  KCORKGION

                                           TPS SAMPLE  #:TS91130    IDEM 8:91130.0

SITE:ST. MARY'S RXVBR          COUNTYrADAMS         LOCATIONS .R.  224  (D/S  6 TT/S)              COLLECTION DATE: 23 - Jul-199
TIME:900hr   LATITUDE/LONGITUDE :40'  50"  £J>«/81'  55"  19.0°       DRAINAGE: 616.5sq.  miles
SEGMENT:21-ST. MARYS RIVER                                  NATURAL REGION CODE:5C-BLUFFTON TILL PLAIN SECTION
TOPOWAP: DECATUR                      HYDROLOGIC UNIT:4100004-ST.  MARY'S RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR .-SPORT  YAK  II NET MESH: 0 . 318cm  SAMPLING DISTANCE: 4 0 Ometers
                             VOLTS: 3.00   DC  CAPTURE DEPTH:< 1.2Ometers  DISTANCE TO SHORE:29 . Ometers

                                         TOTAL NUMBER OF  INDIVIDUALS  COUNTED   132
                                                TOTAL NUMBER OF  TAXA  PRESENT    2§
                                               NUMBER OF  LARGE RIVER  SPECIES     4
                                                    NUMBER OF  DARTER  SPECIES     2.
                                                    NUMBER OF  MADTOM  SPECIES     £
                                                    NUMBER OF SCULPIN  SPECIES     0
                                      NUMBER  OF DARTER/MADTOM/SCULPIN  SPECIES     2.
                                                    NUMBER OF SUNFISH  SPECIES     3_
                                                 NUMBER OF HEADWATER  SPECIES     0
                                                    NUMBER OF  MINNOW  SPECIES     7
                                         NUMBER OF ROUND  BODY  SUCKER  SPECIES     3_
                                              NUMBER OF SUCKER  FAMILY  SPECIES     8
                                                 NUMBER OF SENSITIVE  SPECIES     5_
                                                   NUMBER  OF TOLERANT  SPECIES    11
                 PROPORTION OF  LARGE RIVER  SPECIES  INDIVIDUALS  TO TOTAL COUNT    24.2%
                   PROPORTION OF  HEADWATER  SPECIES  INDIVIDUALS  TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS  TO TOTAL COUNT    61.4%
                                       PROPORTION OF OMNIVORES  TO TOTAL COUNT    33.3%
                                    PROPORTION OF  INSECTIVORES  TO TOTAL COUNT    38.6%
                                      PROPORTION OF CARNIVORES  TO TOTAL COUNT    23.7%
                                        PROPORTION  OF  PIONEERS  TO TOTAL COUNT    22.0%

                               PROPORTION OF SIMPLE LITHOPHILS  TO TOTAL COUNT    25.8%
                                                   PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMONITY IBI DEVELOPMENT DATA
                                               HtJRON-KRIB LAXX PLAIN BCORKGIOR

                                          TPS SAMPLE #:TS91131    IDEM #:91131.0

ITE.-ST. MARY'S RIVER           COUNTY:ADAMS        LOCATION:C.R.  35(W (0/S)                   COLLECTION DATE:23-Jul-1991
1MB:llOShr  LATITUDE/LONGITUDE:40'  53,"  40.0°/85'   0* 30.0°      DRAINAGE:687.Osg.  miles
EGMBNT:21-ST. MARYS RIVER                                   NATURAL REGION CODE: 5C-BLUFFTON TILL PLAIN SECTION
OPOMAP: POE                          HYDROLOGIC ONIT:4100004-ST.  MARY'S RIVER BASIN (MADMEE RIVER BASIN)

                             GEAR:SPORT  YAK II  NET MESH: 0 . 316cm  SAMPLING DISTANCE: 30Procters
                             VOLTS :30J1   DC  CAPTURE DEPTH: < 1.20.meters  DISTANCE TO SHORE:27.Ometera

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   173
                                                TOTAL NUMBER OF TAXA PRESENT    23
                                               NUMBER OF LARGE RIVER SPECIES     3
                                                    NUMBER OF DARTER SPECIES     3.
                                                    NUMBER OF MADTOM SPECIES     1
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     4
                                                   NUMBER OF SUNFISH SPECIES     3.
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     8
                                         NUMBER OF ROUND BODY SUCKER SPECIES     1
                                             NUMBER OF SUCKER FAMILY SPECIES     2
                                                 NUMBER OF SENSITIVE SPECIES     4
                                                  NUMBER OF TOLERANT SPECIES     8
               PROPORTION  OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     4.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                     PROPORTION OF TOLERANTS TO TOTAL COUNT    48.0%
                                     PROPORTION OF OMNIVORES TO TOTAL COUNT    26.0%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    63.6%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT     7.5%
                                       PROPORTION OF PIONEERS TO TOTAL COUNT    37.0%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    21.4%
                                                  PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               RDKOH-KRIE LAKE PLAIN KCORBGION

                                          TPS SAMPLE #:TS91132

SITE.-ST. MARY'S RIVER          COUNTY : ALLBH        LOCATION: HQAGLAND ROAD  (D/S)                COLLECTION DATE :23-Jul-199
TIME:1348hr  LATITUDE/LONGITUDE:±0' 5.6" 14.0°/8S'  5" 11.0°      DRAINAGE:738.Oag.  miles
SEGMENT:21-ST. MARYS RIVER                                  NATURAL REGION CODEiSC-BLUFFTON TILL PLAIN SECTION
TOPOMAP: POE                         HYDROLOGIC UNIT:4100004-ST. MARY'S RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:SPORT YAK  II  NET MESH: 0.818cm  SAMPLING DISTANCE:300meters
                             VOLTS :3_0£  DC   CAPTURE DEPTH :< 1. IQmeters  DISTANCE  TO SHORE : 2 9 . Ometers

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    929
                                                TOTAL NUMBER OF TAXA PRESENT    2S
                                               NUMBER OF LARGE RIVER SPECIES      4
                                                    NUMBER OF DARTER SPECIES      2
                                                    NUMBER OF MADTOM SPECIES      1
                                                   NUMBER OF SCULPIN SPECIES      0.
                                     NUMBER  OF DARTER/MADTOM/SCULPIN SPECIES      3
                                                   NUMBER OF SUNFISH SPECIES      3.
                                                 NUMBER OF HEADWATER SPECIES      0
                                                    NUMBER OF MINNOW SPECIES      T.
                                         NUMBER OF ROUND BODY SUCKER SPECIES      4
                                             NUMBER OF  SUCKER FAMILY SPECIES      6
                                                 NUMBER OF SENSITIVE SPECIES      T_
                                                  NUMBER  OF TOLERANT SPECIES      9
                PROPORTION  OF  LARGE RIVER  SPECIES  INDIVIDUALS TO  TOTAL COUNT      2.0%
                   PROPORTION OF  HEADWATER  SPECIES  INDIVIDUALS TO  TOTAL COUNT      0.0%

                                       PROPORTION OF TOLERANTS TO  TOTAL COUNT     44.1%
                                       PROPORTION OF OMNIVORES TO  TOTAL COUNT     41.4%
                                    PROPORTION OF INSECTIVORES TO  TOTAL COUNT     52.6%
                                      PROPORTION OF CARNIVORES TO  TOTAL COUNT      3.8%
                                        PROPORTION OF  PIONEERS TO  TOTAL COUNT      7.3%

                               PROPORTION OF SIMPLE LITHOPHILS TO  TOTAL COUNT     41.2%
                                                   PERCENT D.E.L.T.  ANOMOLIES      0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMONITY IBI DEVELOPMENT DATA
                                               HURON-ERIE LAKE PLAIN ECOREGIOK

                                          TPS SAMPLE #:TS91133

3ITE:WILBUR DITCH              COUNTY:ALLMf        LOCATION:BHLK ROAD  (U/S)                   COLLECTION DATE-.24-JU1-1991
riME:£30hr   LATITODE/LONGITUDE:41'   2" 34.0*/84' 54" 55.0"      DRAINAGE :5jJ.sg. miles
SEGMENT-.19-MAOMEE RIVBR (MAIN STEM)                          NATURAL REGION CODE:6-BLACK SWAMP NATURAL REGION
[•OPOMAP:  GRAB IL                      HYDROLOGIC ONIT:4100005-MAUMEE RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR: LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE: SOmeters
                             VOLTS:300_  DC  CAPTURE DEPTH:< 0.40meter3  DISTANCE TO SHORE; 3 . Smeters

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   169
                                                TOTAL NUMBER OF TAXA PRESENT    14
                                               NUMBER OF LARGE RIVER SPECIES     0_
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SOJLPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     1
                                                   NUMBER OF SUNFISH SPECIBS     3
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     7
                                         NUMBER OF ROUND BODY SUCKER SPECIES     £
                                             NUMBER OF SUCKER FAMILY SPECIES     1
                                                 NUMBER OF SENSITIVE SPECIES     £
                                                  NUMBER OF TOLERANT SPECIES     7
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                      PROPORTION OF TOLERANTS TO TOTAL COUNT    78.1%
                                      PROPORTION OF OMNIVORES TO TOTAL COUNT    33,7V
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    26.6%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT     2.4%
                                        PROPORTION OF PIONEERS TO TOTAL COUNT    76.9%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT     4.1%
                                                  PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                         INDIANA DEPARTMENT OF  ENVIROHtffiNTAL  MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES  SECTION

                                              FISH COMMUNITY IBI DEVELOPMENT  DATA
                                                BORON-KRIS LAKE PLAIN  HCOREGION

                                          TPS SAMPLE #:TS91134   IDEM #:91134.0

SITE:BBTTSRH DITCH             COUNTY: ALLEH        LOCATION:EHLX ROAD (TT/S)                    COLLECTION DATE :24-Jul-199
TIME:1033hr  LATITUDE/LONGITUDE:41'  2"  29.00/84' 57" 39.0°      DRAINAGE:4.Bag.  miles
SEGMENT :19-MAUMKE RIVER  (MAIN STEM)                         NATURAL REGION CODE:6-BLACK SWAMP NATURAL REGION
TOPOMAP: GRABIL                      HYDROLOGIC UNIT:4100005-MAUMBE RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR: LONGLINE      NET MESH: 0.318cm  SAMPLING DISTANCE : SOmeters
                             VOLTS:30£   DC  CAPTURE DEPTH:< O.lOmeters  DISTANCE TO  SHORE: 1.2meters

                                         TOTAL  NUMBER OF INDIVIDUALS  COUNTED    2T_
                                                TOTAL NUMBER OF TAXA  PRESENT     4
                                                NUMBER OF LARGE RIVER  SPECIES     0
                                                    NUMBER OF  DARTER  SPECIES     0
                                                    NUMBER OF  MADTOM  SPECIES     0
                                                   NUMBER OF SCULPIN  SPECIES     0
                                     NUMBER OF  DARTER/MADTOM/SCULPIN  SPECIES     0
                                                   NUMBER OF SUNFISH  SPECIES     1
                                                 NUMBER OF HEADWATER  SPECIES     £
                                                    NUMBER OF  MINNOW  SPECIES     2.
                                         NUMBER OF ROUND BODY  SUCKER  SPECIES     0
                                              NUMBER OF SUCKER  FAMILY  SPECIES     1
                                                 NUMBER OF SENSITIVE  SPECIES     0
                                                  NUMBER OF TOLERANT  SPECIES     1
                PROPORTION OF LARGE  RIVER SPECIES  INDIVIDUALS  TO TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES  INDIVIDUALS  TO TOTAL COUNT     0.0%

                                      PROPORTION OF TOLERANTS  TO TOTAL COUNT   100.0%
                                      PROPORTION OF OMNIVORES  TO TOTAL COUNT    51.9%
                                   PROPORTION OF INSECTIVORES  TO TOTAL COUNT     3.7%
                                      PROPORTION OF CARNIVORES  TO TOTAL COUNT     0.0%
                                        PROPORTION OF  PIONEERS  TO TOTAL COUNT    92.6%

                              PROPORTION OF  SIMPLE LITHOPHILS  TO TOTAL COUNT     7.4%
                                                   PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HURON- BRIE LAKE PLAIN BCOREQION

                                          TPS SAMPLE #:TS91135

!ITE:SIXMXLB CREEK             COUNTY:ALLKN        LOCATION:PARENT ROAD BRIDGE  (U/S)          COLLECTION DATE:24-Jul-1991
•IMB:llllhr  LATITUDE/LONGITUDE:41'   6." 26.0°/B4' £9" 33.0"      DRAINAGE:5j.fsq. miles
IBGMENT:19-MAUMEE  RIVER (MAIN STEM)                          NATURAL REGION CODEiSC-BLUFFTON TILL PIAIM  SECTION
'OPOMAP: MAPLES                       HYDROLOGIC UNIT:4100005-MAUMEE RIVER BASIN  (MADMEE RIVER BASIN)

                             GEAR:LONGLINE      NET MESH:0.318cm  SAMPLING DISTANCE:6£meters
                             VOLTS :3JX>  DC  CAPTURE DEPTH:< 0.20meter3  DISTANCE TO SHORE:2 . 7meters

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    18
                                                TOTAL NUMBER OF TAXA PRESENT     £
                                               NUMBER OF LARGE RIVER SPECIES     0
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     £
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     1
                                                   NUMBER OF SUNFISH SPECIES     1
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     1
                                         NUMBER OF ROUND BODY SUCKER SPECIES     0_
                                             NUMBER OF SUCKER FAMILY SPECIES     £
                                                 NUMBER OF SENSITIVE SPECIES     0
                                                  NUMBER OF TOLERANT SPECIES     3_
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                      PROPORTION OF TOLERANTS TO TOTAL COUNT    77.8%
                                      PROPORTION OF OMNIVORES TO TOTAL COUNT     5.6%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    88.9%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT     5.6%
                                       PROPORTION OF PIONEERS TO TOTAL COUNT    72.2%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT     0.0%
                                                  PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               BORON-ERIK LAKE PLAIN ECORBQION

                                          TPS SAMPLE #:TS91137

SITE:ST. MARY'S RIVER          COUNTY:ALLBK        LOCATION: S. R. 469  (D/S)                     COLLECTION DATE :25-Jul-199
TIME:840hr   LATITUDE/LONGITUDE-.40' 59_"  V^O'/SB1  5" 45.0°      DRAINAGE:740.5sq. miles
SEGMENT:21-ST. MARYS RIVER                                  NATURAL REGION CODB:5C-BLUFPTON TILL PLAIN SECTION
TOPOMAP: POE                         HYDROLOGIC UNIT:4100004-ST. MARY'S RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:SPORT YAK II  NET MESH: 0 . 318cm  SAMPLING DISTANCE: 300meters
                             VOLTS:3_00  DC  CAPTURE DEPTH:< O.SOmetera  DISTANCE TO  SHORE-.21. Ometers

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED    234
                                                TOTAL NUMBER OF TAXA PRESENT    24
                                               NUMBER OF LARGE RIVER SPECIES     3
                                                    NUMBER OF DARTER SPECIES     3
                                                    NUMBER OF MADTOM SPECIES     0
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     3.
                                                   NUMBER OF SUNFISH SPECIES     2_
                                                 NUMBER  OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     7
                                         NUMBER OF ROUND BODY SUCKER SPECIES     1
                                             NUMBER OF SUCKER FAMILY SPECIES     £
                                                 NUMBER  OF  SENSITIVE SPECIES     5
                                                  NUMBER OF TOLERANT SPECIES     9
                PROPORTION  OF  LARGE RIVER  SPECIES  INDIVIDUALS TO  TOTAL COUNT     2.6%
                   PROPORTION OF  HEADWATER  SPECIES  INDIVIDUALS TO  TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO  TOTAL COUNT     57.7%
                                       PROPORTION OF OMSIVORES TO  TOTAL COUNT     52.1%
                                    PROPORTION OF  INSECTIVORES TO  TOiAL COUNT     38.9%
                                      PROPORTION OF CARNIVORES TO  TOTAL COUNT      5.6%
                                        PROPORTION  OF  PIONEERS TO  TOTAL COUNT     23.9%

                               PROPORTION OF SIMPLE LITHOPHILS TO  TOTAL COUNT     33.3%
                                                   PERCENT D.E.L.T.  ANOMOLIES      0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMUNITY IBI DEVELOPMENT DATA
                                               HUROH-BRIE LAKE PLAIN KCORBGION

                                          TPS SAMPLE tt:TS91138    IDEM ft:91138.0

!ITE:ST. MARY'S RIVKR          COUNTY: AIAHK        LOCATIONS.R. 1 (D/S)                       COLLECTION DATE :25-Jul-1991
'IME:1103hr   LATITUDE/LONGITUDE: 41'   1* 45.0°/8S'  6" 52.0°      DRAINAGE: 780. Oaq. miles
•.EGMENT:21-ST. MARYS RIVER                                  NATURAL REGION CODE:5C-BIiUFFTON TILL PLAIN SECTION
•OPOMAP: FORT WAYNE  WEST             HYDROLOGIC UNIT :4100004-ST. MARY'S RIVER BASIN (MAUMEE RIVER BASIN)

                             GEAR:SPORT YAK II  NET MESH: 0. 318cm  SAMPLING DISTANCE: BOOmeters
                             VOLTS:300   DC  CAPTURE DEPTH:< 0.90meters  DISTANCE TO SHORE:34.Ometers

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   239
                                                TOTAL NUMBER OF TAXA PRESENT    3£
                                               NUMBER OF LARGE RIVER SPECIES     3,
                                                    NUMBER OF DARTER SPECIES     1
                                                    NUMBER OF MADTOM SPECIES     1
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     2
                                                   NUMBER OF SUNFISH SPECIES     £
                                                 NDMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     1
                                         NUMBER OF ROUND BODY SUCKER SPECIES     3.
                                             NUMBER OF SUCKER FAMILY SPECIES     5
                                                 NDMBER OF SENSITIVE SPECIES     £
                                                  NUMBER OF TOLERANT SPECIES    10
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     5.4%
                  PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                      PROPORTION OF TOLERANTS TO TOTAL COUNT    23.0%
                                      PROPORTION OF OMNIVORKS TO TOTAL COUNT    11,3%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    74.9%
                                     PROPORTION OF CARNIVORES TO TOTAL COUNT     8.8%
                                       PROPORTION OF PIONEERS TO TOTAL COUNT    12.1%

                              PROPORTION OF SIMPLE LITHOPHILS TO TOTAL COUNT    51.5%
                                                  PERCENT D.E.L.T. ANOMOLIES     0.0%

-------
                                        INDIANA DEPARTMENT OF ENVIRONMENTAL MANAGEMENT
                                                  OFFICE OF WATER MANAGEMENT
                                                  BIOLOGICAL STUDIES SECTION

                                             FISH COMMONITY IBI DEVELOPMENT DATA
                                               BORON-ERIE LAKE PLAIN SCOREGION

                                          TPS SAMPLE #;TS91139    IDEM #:91139.0

SITE:ST. JOSEPH RTVKR          COUNTY:ALLKN        LOCATION: JOHNNY APPLESKKD PARK              COLLECTION DATE : 25-Jul-199
TIME:1345hr  LATITUDE/LONGITUDE:^'  £" 4S.O°/85'  2"  2-0"      DRAINAGE:*«*.*sg. miles
SEGMENT:20-ST. JOSEPH RIVER                                 NATURAL REGION CODE:5C-BLUFFTON TILL PLAIN SECTION
TOPOMAP: FORT WAYNE EAST             HYDROLOGIC UNIT:4100003-ST. JOSEPH RIVER BASIN  (MAUMEE RIVER BASIN)

                             GEAR:SPORT YAK II  NET MESH:0.318cm  SAMPLING DISTANCE:SOOmeters
                             VOLTS :3_0£  DC  CAPTURE DEPTH: < 1.20meters  DISTANCE TO  SHORE:38.Ometers

                                         TOTAL NUMBER OF INDIVIDUALS COUNTED   730
                                                TOTAL NUMBER OF TAXA PRESENT    33.
                                               NUMBER OF LARGE RIVER SPECIES     4
                                                    NUMBER OF DARTER SPECIES     5
                                                    NUMBER OF MADTOM SPECIES     2_
                                                   NUMBER OF SCULPIN SPECIES     0
                                     NUMBER OF DARTER/MADTOM/SCULPIN SPECIES     7
                                                   NUMBER OF SUNFISH SPECIES     4
                                                 NUMBER OF HEADWATER SPECIES     0
                                                    NUMBER OF MINNOW SPECIES     7_
                                         NUMBER OF ROUND BODY SUCKER SPECIES     5
                                             NUMBER OF SUCKER FAMILY SPECIES     7.
                                                 NUMBER OF SENSITIVE SPECIES    15
                                                  NUMBER OF TOLERANT SPECIES     8
                PROPORTION OF LARGE RIVER SPECIES INDIVIDUALS TO TOTAL COUNT     0.8%
                   PROPORTION OF HEADWATER SPECIES INDIVIDUALS TO TOTAL COUNT     0.0%

                                       PROPORTION OF TOLERANTS TO TOTAL COUNT    43.0%
                                       PROPORTION OF OMNIVORBS TO TOTAL COUNT    39.7%
                                   PROPORTION OF INSECTIVORES TO TOTAL COUNT    55.3%
                                      PROPORTION OP CARNIVORES TO TOTAL COUNT     4.4%
                                        PROPORTION OF PIONEERS TO TOTAL COUNT    10.3%

                               PROPORTION OF  SIMPLE LITHOPHILS TO TOTAL COUNT    46.2%
                                                  PERCENT  D.E.L.T. ANOMOLIES     0.0%

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APPENDIX  C.  Fish nomenclature changes for the species of fish occurring within the poUtical
                boundaries of Indiana.
Petromyzontifonnes - lampreys
  Petromyzontidae - lamprey
Lampetra appendix (DeKay), American brook lamprey
Lepisosteiformes - gars
  Lepisosteidae - gars
Atractosteus spatula (Lacepede), alligator gar
Salmoniformes - trout, salmon, whitefish
Salmonidae - trout, salmon, whitefish
Oncorhynchus mykiss Walbaum, rainbow trout
Cypriniformes - carps and minnows
  Cyprinidae -carps and minnows
Campostoma oligolepis Hubos and Greene, largescale stoneroller

Cyprinella lutrensis (Baud and Girard), red shiner
Cyprinella spiloptera Cope, spotfin shiner
Cyprinella whipplei (Girard), steelcolor shiner
Erimystax dissimilis Rutland, streamline chub
Erimystax x-punctata Hubbs and Crowe, gravel chub
Extrarius aesttvalis Girard, speckled chub
Hybopsis amnis Hubbs and Greene, pallid shiner
Luxilus chrysocephalus (Rafinesque), striped shiner
Luxilus cornutus (Mitchell), common shiner
Lythrurus ardens (Cope), rosefin shiner
Lythrurus fumeusEvenaaan, ribbon shiner
Lythrurus umbratilis (Girard), redfin shiner
Macrhybopsis storeriana (Kirkland), silver chub
Notropis ludibuundus Cope, sand shiner
Opsopoeodus emiliae Hay, pugnose minnow
SUuriformes - bullhead and catfish
  Ictahiridae - bullhead and catfish
Ameiurus catus (Linnaeus), white catfish
Ameiurus melas (Rafinesque), black bullhead
Ameiurus natalis (Lesueur), yellow bullhead
Ameiurus nebulosus (Lesueur), brown bullhead
Atheriniformes - topminnows, silversides
  Fundulidae - topminnows
Pereiformes - basses, sunfish, perch, darters
  Moronidae - temperate basses
Morone chrysops (Rafinesque), white bass
Morone mississippiensis Jordan and Eigenmann, yellow bass
Morone saxatilis (Walbaum), striped bass
  Elassomatidae -  pygmy sunfish
Elassoma zonatum  Jordan, banded pygmy sunfish
  Percidae - perches and darters
Crystallaria asprella Jordan, crystal darter
Previous
Nomenclature

Lampetra lamottei
Lepisosteus spatula


Salmo gairdneri
previously considered
Campostoma anomalum pullum
Notropis lutrensis
Notropis spiloptera
Notropis whipplei
Hybopsis dissimilis
Hybopsis x-punctata
Hybopsis aesttvalis
Notropis amnis
Notropis chrysocephalus
Notropis cornutus
Notropis ardens
Notropis fitmeus
Notropis umbratilis
Hybopsis storeriana
Notropis stramineus
Notropis emiliae
Ictalurus catus
Ictalurus melas
Ictalurus natalis
Ictalurus nebulosus

previously Cyprinodontidae
previously Percichthyidae
previously Centrarchidae
Ammocrypta asprella

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             REPORT  DOCUMENTATION PAGE
                                                        Form Approved
                                                        OMB NO. 0704-0188
 Public reporting Burden tor this collection of intormation is estimated to average i hour per response, including the time tor reviewing instructions, searching existing data sources.
 gathering and maintaining the data needed, ana completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this
 collection or information, including suggestions tor reducing this burden to Washington Headquarters Services. Directorate for Information Operations and Reports. 1215 Jefferson
 Davis Highway. Suite 1204, Arlington, VA 22202-4302. and to the Office o* Manaaemem and Budget, Paperwor* Reduction Protest (0704-0188). Washington. DC 20503.
 1. AGENCY USE ONLY (Leave blank)
          2. REPORT DATE
           December 1994
  3. REPORT TYPE AND DATES COVERED
    Final
 4. TITLE AND SUBTITLE
   Development of  Index of  Biotic  Integrity Expectations
   for the Ecoregions  of Indiana.   II. Huron-Erie  Lake
   Plain
 6. AUTHOR(S)

   Thomas P.  Simon
                                                                     5. FUNDING NUMBERS
 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES)
   U.S.  Environmental  Protection  Agency,  Region
   Water Division
   Watershed  and Non-Point  Source Branch
   77 West Jackson  Boulevard, WW-16J
   Chicago,  Illinois   60604	 	
                                                 8. PERFORMING ORGANIZATION
                                                   REPORT NUMBER
                                                    EPA 905/R-92-007
 9. SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES)

    same as  # 7
                                                 10. SPONSORING/MONITORING
                                                    AGENCY REPORT NUMBER
 11. SUPPLEMENTARY NOTES
   Prepared  in cooperation  with  the Indiana Department  of Environmental  Management,
   Surveillance and  Standards Branch
 12a. DISTRIBUTION / AVAILABILITY STATEMENT
    UNLIMITED
                                                                     12b. DISTRIBUTION CODE
                                                     UNLIMITED
 13. ABSTRACT (Maximum 200 words)
   The Maumee River drainage was  investigated during 1992 to determine  water
    resource expectations  for headwater and wadable streams.  A  total of 77 sites
   were sampled within  three watersheds  to develop and  calibrate an Index of  Biotic
    Integrity for  use in the Indiana portion of  the watershed. ^Maximum  species
    richness lines  were  developed  for streams less  than  2000 mi   drainage area.  The
   Maumee  River possesses  a highly skewed  IBI indicating  lower  extremes in water
    resource integrity in  the headwater streams.   The St.  Joseph River draiange  had
    the highest integrity  of the three watersheds.   Site specific data including
    an evaluation  of fish  community trends, tolerance,classifications, trophic and
    reproductive guilds  are included.
 14. SUBJECT TERMS

    Maumee  River,
    criteria, IBI
St. Joseph River, St.  Marys  River,  biological
 fish  community 'structure,  Indiana
                                                        15. NUMBER OF PAGES
                                                           68 +  appendices
                         16. PRICE CODE
 17. SECURITY CLASSIFICATION
    OF REPORT

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         OF THIS PAGE

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f9. SECURITY CLASSIFICATION
   OF ABSTRACT

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20. LIMITATION OF ABSTRACT
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NSN 7540-01-280-5500
                                                                         Standard Form 298 (Rev
                                                                         prescribed by ANSI Std  Z39-18
                                                                        2-89)

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