EPA-460/3-74-022
     STUDY  OF  POLYNUCLEAR
    AROMATIC HYDROCARBON
EMISSIONS  FROM HEAVY DUTY
           DIESEL ENGINES
                  Prepared by

                  R. S. Spindt

         Gulf Research and Development Company
                P.O. Drawer 2038
           Pittsburgh, Pennsylvania 15230


              Contract No. 68-01-2116


               EPA Project Officer:

                 R. E. Maxwell


                  Prepared for

          Coordinating Research Council, Inc.
               30 Rockefeller Plaza
             New York, New York 10020

                     and

       U.S. ENVIRONMENTAL PROTECTION AGENCY
          Office of Air and Waste Management
       Office of Mobile Source Air Pollution Control
          Emission Control Technology Division
             Ann Arbor, Michigan 48105

                   July 1974

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This report is issued by the Environmental Protection Agency to report
technical data of interest to a limited number of readers.  Copies are available
free of charge to Federal employees, current contractors  and grantees,
and nonprofit organizations-as supplies permit-from the  Air Pollution
Technical Information Center, Environmental Protection Agency, Research
Triangle Park, North Carolina 27711; or, for a fee, from  the National Technical
Information Service,  5285 Port Royal Road, Springfield, Virginia 22161.
This report was furnished to the Environmental Protection Agency by
Gulf Research and Development Co. , Pittsburgh , Pa . , in fulfillment of
Contract No. 68-01-2116.  The  contents of this report arc reproduced herein
as received from Gulf Research and Development Co. The opinions, findings,
and conclusions expressed are those of the author and not necessarily
those of the Environmental Protection Agency. Mention of company or
product names is not to be considered  as  an endorsement by the Environmental
Protection Agency.
                   Publication No. EPA-460/3-74-022
                                     11

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            FIRST ANNUAL REPORT
                    ON
POLYNUCLEAR AROMATIC CONTENT OF HEAVY DUTY
        DIESEL ENGINE EXHAUST GASES
             Submitted to The
      Environmental Protection Agency
          Contract No. 68-01-2116

                  And The

    Coordinating Research Council, Inc.
  CRC-APRAC Contract No. CAPE-24-72(l-73)
                 731-CD003
                For Period
            Ending July 1, 1974
                    By
               R.  S.  Spindt
    Gulf Research  & Development Company
             P.  0.  Drawer 2038
           Pittsburgh,  PA 15230

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                          TABLE OF CONTENTS

                                                                    Page
LIST OF TABLES	  iii
LIST OF FIGURES	   iv
ABBREVIATIONS	    v
PERSONNEL	    1


  I.  SUMMARY	    2
 II.  INTRODUCTION	    4
III.  EXPERIMENTAL	    6
     A.  Analytical Procedures	    6
     B.  Bench Studies to Validate Sampling Techniques	    6
     C.  Engine Studies	   10
 IV.  RESULTS	   13
     A.  Exhaust Sample Collection Development	   13
     B.  Application of Radiotracers	   17
     C.  Analysis of Diesel Fuels	   22
     D.  Engine Repeatability Studies	   28
     E.  Additional Engine Studies	   30
     F.  Comparison of Exhaust PNA and Fuel PNA	   33
     G.  Comparison of Gasoline and Diesel PNA Emissions	   36
     H.  Analysis of Gaseous Emissions	   38
  V.  CONCLUSIONS	   40
 VI.  REFERENCES	   41

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                                                                          ii



                      TABLE OF CONTENTS - CONTINUED
APPENDIX A - ANALYTICAL PROCEDURES	 42




      I.  PNA HYDROCARBONS IN DIESEL FUEL	 42




          A.  PNA Concentration	 42




          B.  Thin Layer Separation	 45




          C.  Cellulose "Prep" Plate	 45




          D.  Cellulose Separation	 46




          E.  Recovery	 47




     II.  DIESEL EXHAUST GAS ANALYSIS	 49




          A.  Chromosorb 102	 49




          B.  Lines	 49




          C.  Filter Paper	 49




          D.  PNA Concentration	 51




    III.  PHENOL ANALYSIS	 53




          A.  Preparation of Phenol Concentrate by Caustic Extraction..53




          B.  Total Phenol Analysis	 54




               1.  Color Development	 54




               2.  Calculations	 54




               3.  Application of Total Colorimetric Phenol Method	 55




          C.  Chromatographic Analysis	 55




          D.  Methods Comparison	 55






APPENDIX B - Diesel Engine Operating Procedures	  61




APPENDIX C - Gaseous Emissions Summary	  63

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                                                                    iii

                            LIST OF TABLES

Table No.                       Title                                Page
     1        Measurement of BaP Recovery Using Chromosorb-102	  9
     2        Engine Studies of PNA Recovery	 14
     3        Analysis of Radiotracer and UV Results,Exp.ll	 19
     4        Engine Productd BaP	 21
     5        Recovery of Injected BaP	 23
     6        Survey Fuel Inspection Data	 26
     7        PWA Content of Diesel Fuels ug/kg	 27
     8        13-Mode Test Repeatability	 29
     9        PNA Recovery in Double Length 13-Mode Cycle Test	 31
    10        PNA Emissions From The Smoke Cycle	 32
    11        Average PNA Emissions From Four 13-Mode Cycles	 34
    12        Comparison of PNA Found With Fuels 2-5 And 2-16	 35
    13        Comparison of Exhaust PNA to PNA in Fuel	 37
    14        PEA Emissions From Diesel and Gasoline Engines	 38
    15        Gaseous Engine Emissions	 39
   A-l        PNA Recovery-Alumina, Neut.,  2.5$ Water Deactivated.... 44
   A-2        PNA Recovery Study - No. 2 Fuel Oil	 48
   A-3        Total Phenols	 57
   A-4        Gas Chromatographic Operating Conditions	 58
   A-5        Gas Chromatographic Separation of Phenols	 59
   C-l        Gaseous Emission Summary - 13-Mode Cycle	 63

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                                                                          iv





                            LIST OF FIGURES
Figure No.                                                               Fa





     1        System for Validating PKA Collection Efficiency	  7




     2        Chromosorb-102 Trap	  8




     3        Proportional Sampling System	12




     4        PNA Collection Systems	15




     5        Standardized Sampling System	24






    A-l       Diesel Fuel PNA Separation Procedure	  43




    A-2       Exhaust Gas Sample Preparation	  50




    A-3       Exhaust Gas Sample PNA Separation	  52




    A-4       Gas Chromatograph of Phenols in Diesel Exhaust	  56

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                           ABBREVIATIONS
APRAC    -  Air Pollution Research Advisory Committee


Anth     -  Anthracene


BaA      -  Benz(a)anthracene


BaP      -  Benzo(a)pyrene

 14
C  BaP   -  Radioactive Benzo(a)pyrene


BeP      -  Benzo(e)pyrene


BghiP    -  Benzo(g,h,i)perylene


Chry     -  Chrysene


DDAD     -  Detroit Diesel Allison Division


Fluo     -  Fluoranthene


10       -  Isooctane


M-Anth   -  Methyl Anthracene


PNA      -  Polynuclear Aromatic Hydrocarbons


Phen     -  Phenanthrene


Pyr      -  Pyrene


TLC      -  Thin Layer Chromatography


V-V      -  Volume/Volume

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                              PERSONNEL
                                                                            1.
Supervisory Project Group

       Mr. Roger Bascom - Chairman

       Mr. C. R. Begeman

       Mr. D. S. Gray

       Mr. R. J. Hames


       Mr. M. Malbin

       Mr. R. E. Maxwell

       Dr. S. S. Hetrick

       Dr. Margaret Griffing

       Dr. H. K. Newhall

       Dr. J. M. Perez
Cummins Engine Company

General Motors Research Laboratories

AMOCO Oil Company

Detroit Diesel Allison Division,
General Motors Corporation

Shell Development Company

Environmental Protection Agency

Mobil Research and Development

Ethyl Corporation

Chevron Research Company

Caterpillar Tractor Company
Gulf Research & Development Company Personnel:

            Dr. R. S. Spindt     -  Project Manager

            Mr. J. C. Mulac, Jr. -  Senior Project Engineer

            Mr. J. G. Berry, Jr. -  Project Engineer

            Mr. M. S. Norris     -  Senior Research Chemist

            Mr. E. D. Hill       -  Research Chemist

            Mr. E. S. Dorinsky   -  Technician

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                                                                           2.





 I.    SUMMARY




           The first phase of a CRC/EPA sponsored study of polynuclear




 aromatic (PNA)  and  phenolic  hydrocarbon emissions from diesel  engines has




 been completed by Gulf Research & Development  Company.   The  first  year's




 effort was concentrated on validation of the sample  collection and




 analytical techniques  and a  survey of the PNA  content  of U.S.  diesel  fuels.




           A collection system consisting of a  precooler,  a glass fiber




 particulate filter,  and a Chromosorb-102* trap was used to collect PNA1s




 from diesel exhaust.   PNA's  are removed from the collection  system by




 solvent washing and soxhlet  extraction,  concentrated,  and analyzed by thin




 layer chromatography.   Total phenols  are  analyzed by the  4-amino-antipyrene




 procedure.




           Attempts  to  validate  the  sample collection system  using  C




 tracers were  not successful.   It is hypothesized that PNA's  injected  into




 the  exhaust (i.e.,  tracers)  are largely destroyed by reactions with other




 exhaust components, while engine-generated PNA's  on  or  within  soot particles




 survive these reactions.   Radio tracers also indicate that PNA-destroying




 reactions   occur   even  after collection  and extraction from the sample




 system.  Similar losses  of PNA's  do not occur  with synthetic exhaust.




           The fuel  analyses  showed that ASTM 1-D diesel fuels are  nearly




 free  of PNA compounds.   PNA  levels in ASTM 2-D diesel fuels ranged from




 near  zero  to  values that  reach  the average concentration   reported in motor




 gasolines.








*Chromosorb-102  is a registered trademark of Johns-Manville,  Inc.

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          Work has been started to determine the effect of operating




conditions on PHA emissions and to establish the repeatability of the




overall procedure.  Future work will concentrate on measurement of PNA




emissions from other types of diesel engines and on identifying the effects




of specific operating conditions,  blowby,  deposits, lube oil,  and fuels.

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                                                                         4.
II.  INTRODUCTION



          Concern for air pollution has shifted from major pollution



sources, first subjected to control measures, to include those of lesser



magnitude as the major sources have come under control.  Diesel engine



exhaust is still a minor factor in pollution in the United States.  But



because the diesel engine produces both smoke and odor in the exhaust,



attention to this problem area is justified.



          Polynuclear Aromatic Hydrocarbons (PNA) do not appear to occur



naturally in the environment but have been associated with combustion

          / -i  P w              (i }

processes/ '     Ray and Long^ ' state that PNA is associated with soot


                                                    (2)
formation and inefficient combustion.  Bailey, et al    states that PNA



in diesel engines is associated with overfueling.


                                 (5}
          A Public Health Servicev ' study indicated that hand fired coal



burning combustion sources are the most important source of BaP emissions



in the United States. Much lower emissions were estimated for all other



combustion sources including motor vehicles.



          An important investigation of the automotive vehicle contribution



to BaP levels in the atmosphere has been completed by Colucci and Begeman. '



They showed that vehicular contributions to total PNA levels could account
for between 5 and 42/0 based on correlations with lead measurements.  In



another study, these authors^ ' showed that BaP in the fuel can survive the


                                                             (6)
combustion processes in a gasoline engine (up to 36^).  Gross^    in a study



for the CRC-APRAC, showed that PNA emissions from gasoline engines were



influenced by (a) emission control systems, (b) PNA in fuel,  and (c) oil



consumption rate.





 ^Numbers in parenthesis designate References at end of report.

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                                                                         5.






          As a result of the extensive PNA studies that have been made




on the gasoline engine and the relatively little work that has been done




on the diesel engine, the EPA and CRC-APRAC, in the fall of 1972, agreed




to fund a project to investigate PNA in diesel exhaust gases.  The project




was awarded to Gulf Research 8= Development Company with a starting date




of June 29, 1973.




          The scope of work for this project envisioned a two-year program




with specific items clearly spelled out.  The eight items included are as




follows:




               1.  Demonstrate and validate the sample collection and




                   analytical techniques.




               2.  Survey a number of fuels and select a baseline fuel.




               3.  Measure PNA emissions from selected (7) engines.




               4.  Measure PNA emissions by operating mode.




               5.  Determine PNA emissions with fuels of high and low




                   PNA content.




               6.  Evaluate deposit effects on PNA emissions.




               7.  Determine the accumulation of PNA in the oil.




               8.  Evaluate effect of exhaust gas recirculation.






          Only the first four items were to be included in the first year's




contract.  Tasks 3 and 4 were not completed under this contract due to the




increased expenditure required to resolve problems encountered in Task 1.

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                                                                          6.
III.  EXPERIMENTAL





     A.  Analytical Procedures




          We chose to determine polynuclear aromatic hydrocarbons (PEA)




and phenols by a separation technique that includes liquid solid column




chromotography and thin layer chromotography.  Details of the procedures




are given in Appendix A.




          It was anticipated that the engine exhaust sample would be




collected using a filter and a chromosorb-102 adsorption trap.  '  This




material is known to adsorb hydrocarbons readily.  Simple elution with




benzene was expected to recover the FNA quantitatively from the chromosorb




for further separation.




     B.  Bench Studies to Validate Sampling Techniques




          To test the efficiency of the proposed trapping system for




PNA, a heated gas flow system was constructed as shown in Figure 1.   The




construction of the sampling trap is shown in Figure 2.  Air or nitrogen




gas at 3 CFM (5 m3/hr.) heated to an exit temperature of 350°F (177°C),



was spiked with benzo(a)pyrene using a motor driven syringe.   Five jug Bap




in 1 ml. of isooctane was introduced into the gas stream over a 2-hour




period, except for runs B8-10.




          Table 1 lists the results of these tests for several operating




conditions.

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                        FIGURE I
SYSTEM FOR VALIDATING PNA COLLECTION  EFFICIENCY
                 Heating Coil
                  Chromosorb.
                  102 Traps
        Water
       Saturator
                                  Motor  Driven Syringe
                                  For Injecting BaP
                                     Thermocouple
D
Dry Gas
Flow Meter

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                                         Fl GURE 2
                                CHROMOSORB  102 TRAP
      .S.S. Pressure Cyl.
      End Cap
-Screen  Assemblies
 100 Mesh Welded   piston Ring
3" S.S. Tube
(3"O.D. x  -  Wall)
-Half of |- NPT
 Collar
                        3" Marman
                        Flange (4 Req'd.)
                                             3" S.S. Tube
                                             (3"O.D. x JL wall)
                                                                                                CD

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                                                                               9.
                                    TABLE 1
                  MEASUREMENT OF BaP RECOVERY USING CHROMOSORB-102
Test
B-l
B-2
B-3
B-4
B-5
B-6
B-7
B-8
B-9
Gas'1'
Temp.
300 °F
300 °F
300 °F
300 °F
350 °F
350 °F
350 °F
350 °F
350 °F
Added Components


Water Vapor
Water Vapor
Water Vapor
Phenol in 10 + Water Vapor
m-Cresol in 10 + Water Vapor
Preload trap with 5 ug BaP
Preload trap
Carrier Recovery Efficiency %
Gas BaP Phenol
N2 89.
W2 89.
W2 74.
N 85.
N2 ?6'
N2 90. 0
N 96. 23$ (in
water trap)
Air 91.
Air + 87.
720 ppm NO
B-10    350°F    Preload trap inject 1 ml ether
                  + diesel fuel (10/0)
Air +      85.
900 ppm NO
(1) Temperature measured at BaP injection point.
              In each case, a second chromosorb trap was installed in the system

    but no BaP was ever found on it.  Therefore, Chromosorb-102 is an excellent

    scavenger for BaP.  The temperature of 350°F (177°C) was chosen because at

    lower temperature, some of the BaP is found in the lines.  A recovery of

        is quite satisfactory.

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                                                                           10.





          Phenol or m-cresol at 100 mg/ml was added with the BaP in two



experiments.  In neither case, did the presence of these compounds interfere



with the recovery of BaP.



          The three runs with air as the carrier gas showed that no BaP is



flushed from the Chromesorb-102 trap.  However, the addition of NO to the



air seems to indicate a possible degradation of the recovery.  Since these



runs were all of short duration (15 minutes), it is possible that longer



exposure would have increased the loss.



          In these experiments, the syringe, lines and traps are separately



washed with benzene and the separate solutions concentrated with 1 ml



hexadecane.  The final hexadecane solution was then analyzed using ultra-



violet spectroscopy.  Since only one PNA was present, no separation



technique was required, except when ether and diesel fuel were added.



     C.  Engine Studies



          A Detroit Diesel Allison Division (DDAD) model 3-53 automotive



engine was available in this laboratory for our initial studies.  The engine



test cell instrumentation included equipment for measuring: (1) engine torque



and speed, (2) fuel consumption, (3) gaseous emissions (CO, HC, WO, NO ,
                                                                      A


C00, and 0_), and (4) smoke.  The optical portion of the smokemeter was
  t»       Lt


mounted at the end of the exhaust stack approximately 25 feet (7.6 m) from



the engine.  Two sample probes were installed in the engine exhaust pipe



approximately 10 feet (3m) from the engine.  One probe supplied exhaust



sample for the standard gaseous emission measurements and the second probe



supplied sample for PNA analysis.  A variable rate proportional sampler was

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                                                                            11.
modified to permit varying the ratio of sample gas volume to total exhaust



gas volume.  This alteration allows the use of the device on any size



engine.  Figure 3 is a diagram of the exhaust sampling system.



          Two cycles were specified for evaluation in this work.  These


                                        (8)
cycles are given in the Federal Register    in complete detail.  Appendix



B describes the speed load pattern with time that was used.  The 13-mode



Federal Cycle for standard exhaust pollutant analysis was used for



preliminary studies.  This cycle can be sampled at constant flow rate,



proportional to the engine exhaust flow because it is a series of steady



state conditions.  The other cycle is the Federal Smoke Cycle composed of



transient acceleration and lugging modes.  Since this composite transient



cycle must be repeated approximately one hundred times, a DATA-TRAK controller



was programmed to duplicate this cycle.  This cycle controller with the



variable rate proportional sampler makes the collection of a constant fraction



of the exhaust relatively easy.

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                               FIGURE 3
                   PROPORTIONAL  SAMPLING  SYSTEM
   Participate
     Filter
   Chromosorb
    102PNA
     Trap

Heat Exchanger
 8 Water Trap
     35°F
                                                  Engine Air
                                              Laminar Flow Element
                                                   0-600cfm
                                        Proportional  Sampler
                                          Control System
Dry Gas  Meter
          Servo Operated
          Sampling Valve
                                 Exhaust Sample
                              Laminar Flow Element
                                    0-20 cfm

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                                                                             13.




IV.  RESULTS




     A.  Exhaust Sample Collection Developments




          Table 2 summarizes the results of tests to analyze the PKA emissions




and to evaluate the collection and analysis schemes.  All tests were run with




the DDAD 3-53 engine.  The results have not been corrected for analytical losses.




          Runs 1 and 2 show results of PNA collection using a direct filtration




procedure (Figure 4-A).  These data appear to be remarkably reproducible.




          Starting with Experiment 3, various PNA were either injected into




the exhaust gas or predeposited on the chromosorb to compare the recovery




obtained to that in the bench tests.  Heated sample lines were used in




Experiments 3 through 5 (Figure 4-B) because work with the bench studies




indicated that a temperatue of 350°F (177°C) was needed to ensure that the




ERA would be in the vapor phase.  Only the injected compounds (or those




predeposited on the chromosorb) were isolated.




          The failure to recover any BaP in Run 3 was surprising and should




have been a warning that heating the sample might be a problem.  The maximum




temperature at the point of taking the sample from the stack varied, depending




on engine operating conditions, from 130 to 850°F (54 to 454°C).  Run 4 is a




strong indication that BaP is partially destroyed in the sampling system, that




is, less than 50$ of the injected BaP was recovered compared to the approximately




90$ in the bench tests.  Run 5 indicates that destruction of BeP can occur




and that BaP predeposited on the chromosorb is partially lost.  Because, in




bench studies, BaP on the chromsorb 102 trap was recovered in 90$ yield, the 20$




recovered in Run  5 probably means that most of the BaP reacted with constituents




in the exhaust gases.  Similar loss of predeposited BaP on the chromosorb was




observed in Runs 6, 7, and 9.

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                                                                                TABLE 2
ENGINE STUDIES OF PliA RECOVERY
Engine DDAD-3-53 - Fuel 2-5

Exp.
No.
1
2
3
4
5
6
7
8
9
10
11

12
13
14
15
16
17
18
19
20
21

Collection System (Figure No.)
Direct Filtration and Chromosorb-102 (4-A)
Direct Filtration and Chromosorb-102 (4-A)
Heated line (350°), Filtration, Chrom.-102 (4-B)
Heated line (350°), Filtration, Chrom.-102 (4-B)
Heated line (350°), Filtration, Chrom.-102 (4-B)
Condenser 4 Filter 4 Chromosorb-102 (4-C)
Condenser 4 Filter 4 Chrom.-102 (C16H34 on Filter )( 4-C )
Ammonia injection to determine whether NO is destroyed.
Air dil. (4/1) Temp. 75° 4 Filtration 4 Chrom.-102 (4-D)
Air dilution (4/1) (4-D)
Air dilution (4/1) Temp. -20° + filtration. (4-D)

Condenser 4 filter + Chromosorb-102 (4-C)
Heated line - Stack Sampling Procedure (4-E)
Cryogenic (-90°) bath + filtration 4 Chrom.-102, heated
line. Methanol injected. (4-F)
Cryogenic (-90°) bath 4 filtration + Chrom.-102
Methanol injected. (4-F)
Cryogenic (-90°) bath 4 filtration 4 Chrom.-102
(No methanol.) (4-F)
Precooler, cryogenic trap, filter (4-F)
Precooler, filtration + Chromosorb-102 (5)
Precooler, filtration + Chromosorb-102 (5)
Precooler, cryogenic trap 4 filter -
Ammoniated Methanol injected. (4-F)
Precooler, cryogenic trap 4 filter -
Ammoniated Methanol injected. (4-F)

Engine
Cycle
13-mode
13-mode
13-mode
13-mode
13-mode
13-mode
13-mode

1800 rpm
1/2 load
1800 rpm
1/2 load
1800 rpm
1/2 load

13-mode
1800 rpm
1/2 load
1800 rpm
1/2 load
13-mode
13-mode
13-mode
13-mode
13-mode
13-mode
l-3mode

Injected
crop
-
BaP
BaP
BeP
Bap(l)
BeP
Bap(l)
BeP
Bap(l)

BeP
Bepd)
C14BaP
BeP
Bap(l)
BepU)
BaP
BaA
BaP
BaA
BaP
BeP
-
-
C14BaP
-
C14BaP
C14BaP
C14BaP

Compound
JJg_

6
4.4
5.27
5
2.1
5
4.25
5

9.11
4.67
9.90
5.73
6.42
3.97
4.0
3.8
6.22
5.35
6.45
5.47
6.68
5.72


1.30

0.98
1.30
1.26

Recovery - jug/Test
BaP BeP BaA BghiP
0.56 0.66 O.S9 3.72
0.48 0.62 0.86 3.45
0 -
2.38 -
1.11 1.95
2.03 1.5
1.9 0.37

1.93 6.62
2.62 4.24
3.59(2) 3.57(2)
3.22*(2)
1.62(3) 3.83(3)

2.P6 - 1.6o(2)
0.0 (3) o.O (3)
0.64 - Trace
3.43 1.62 0 .96
0.16 0.69 0 0.14
0.39 0.23 Trace 0.20
0.45 000
0.04*
0.38 0.27 Trace 0.72
0.59 1.46 1.14 0.30
0.14*
0000
1.01 0.71 1.37 0.5S
0.22*
Exhaust
Volume
Sampled
7.50
7.67
7.39
7.67
6.34
6.40
6.88
-
6.80
6.51
5.24

6.97
5.21
6.80
7.31
8.30
7.99
6.85
7.76
7.76
7.90

Remarks


Injected over total cycle.
Injected during last 10 min
Injection during Mode 11.
Injection during Mode 10
and 11 (10 min.)
Injection during Mode 6
Condenser water not on.
No loss of HOX found.
Injection over whole test
(rest of runs).






Difficult to recover from
glass wool in trap.
Difficult to recover from
glass wool in trap.
Injection ahead of pre-
cooler temp .> 400 °F in Ng
stream.
Like #1 and 2.
Injection after precooler.
Lost.
Replaced Cu cooling coil
with stainless steel.
(1) Compound placed on Chromosorb-102 before  starting run.
 *Radioactive BaP.
(2)  Recovered from filter and lines.
(3) Recovered from Chromosorb-102.
                                                                                                                                                         RSS-1974

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                                      FIGURE 4
                             PNA  COLLECTION  SYSTEMS
                                                                                           15.
                4A
                     4B
          75°F-340°F
         (2O°C-I7I°F)
             40°F(4.4°C)
                                                                   Heated Line
                                                                   350°F (I77°C)
                                                               40°F (4.4°C)
                                                                                       LU
                 4C
      75°F-340°F
      (24°C-I7I°C)
                           40°F(4.4°C)
3508F(I77°C)
     N2
                                                   3L/Min.
                                                   Run II
                                                                     4D

                                                                 -20°F(-29°C) RUN II
                                                                  I40°F(60°C)RUN 9810
                                                               65°F(I8°C)
                                                               RUN  9 8 10
                               -40°F
                              (-40°C)
                               RUN II
                                                                            AIR
NOTE:  Qiass Wool in Necks of
       Last Two Traps.
                              Dry Ice - Acetone
                        Ice a Salt Water
                                                         200 ml. Ethanol
                                                                     I30°F-300°F
                                                                     (540C-I49°C)
  LEGEND

Syringe Injector

Participate
Filter
Chromosorb 102
Trap

Condenser

Liquid Trap
                                                                      Pre-Cooler
                                                                      60°F Water
                                                                    Methanol
                                                                  Where Applicable
                                     Trap
                          NOTE: Steel or Glass
                                Wool in Both
                    Trap        Traps.

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                                                                             16.
          Because BaP recovery was low with a heated sample line,  it was




decided that cooling the exhaust gas sample might be beneficial.   Runs 6,




1, and 12 use a heat exchanger ahead of the filter (Figure 4-C) to lower




the sample temperature.  In Run 6, BeP loss was only 30$ but BaP loss was




60/o.  In Run 7 the losses were 90$ and 60% respectively, possibly because




water was not used in the heat exchanger.  In Run 12, (• •- 35$) of both BaP




and BaA was recovered. By separate analyses in Run 12,  approximately equal




amounts of these compounds were found in the sampling lines, condenser and




condensate, and on the filter.  There was none of either compound on the




Chromosorb-102 trap, suggesting that this trap is unnecessary for recovery




of the PNA hydrocarbons.  However, it is known that phenols do collect on




this trap.



          Since a water-cooled condenser did not significantly improve the




total recovery, an air dilution system was tried (Figure 4-D).  In these




experiments (9, 10, and 11), instrument air, passed through a Chromosorb-102




trap to remove hydrocarbons, was introduced into the sampling system so that




a 4/1 ratio of air to exhaust gas flow was maintained.   These air dilution




experiments were run at constant engine speed and load where it is easy to




maintain steady flow.  It would be possible to do the same thing for the




13-mode cycle but quite impractical to use air dilution with the smoke cycle




and the proportional sampler.




          In Run 9 an approximate 65$ recovery of injected BeP was obtained,




whereas in Run 10 the recovery of BaP was about 20$.  Run 11, in which the




added air was about -40°F (-40C), resulted in a sample stream temperature




of -20F (-29°C).  This run resulted in the best recovery of BaP,  50$ of the




amount injected.  One problem encountered was the occasional freezing of the

-------
                                                                             17.
 exhaust stream as  it mixed with the  cold air.   A solution to this  problem




 was  not found.




           Calculating  the  recovery of injected PKA from these experiments




 is complicated by  not  knowing exactly how much was produced in the engine. .




 Because we think the injected BaP greatly exceed the  BaP from the  engine,




 we have ignored the  engine produced  BaP in  estimating PNA recovery.





      B.  Application of Radiotracers




           The  Project  Group had been  urging Gulf Research & Development




 Company to employ  radioactive tracers to help  understand what was  happening




 during  sampling.   This approach had not been pursued  because  the analytical




 equipment  was not  available at Gulf Research & Development Company.  In




 March 1974, representatives of the Ethyl Corporation  and Chevron Research then




 volunteered to  do  the radioassays.  Later,  we  arranged  for New England Nuclear




 Company to do this analysis on a routine basis.   Through the  courtesy of




 Dr. Margaret Griffing of the  Ethyl Corporation,  the Contractor was supplied




 with  a  quantity of radioactive benzo(a)pyrene  (C14BaP).   The  C14:BaP permitted




 us to distinguish between BaP injected  and  BaP produced by the engine.  The




 high  purity of  the C  BaP was  established by UV  spectral  analysis.




           The use of C14BaP in Experiment 11 showed several things.  Both




 C14BaP  (6.42 ;ug) and BeP (3.97 ug) were injected while non-radioactive




BaP (4.0/ig) and BeP (3.8 ug) were predeposited on the chromosorb trap.   Upon




analysis, 3.57 jag of BeP (~ 74$) was  recovered from the lines and filter while




3.83 jug Bep (~ 100$) was recovered from the Chromosorb-102 trap.  This recovery




of BeP is considered excellent.

-------
                                                                        18.



                                            14
          Table 3 gives an analysis of the C  BaP distribution found in



Experiment 11.  Of the injected C  BaP, 50$ was recovered from the lines and



filters, 2.2$ in the BaP fraction from the chromosorb,  and 21.5$ in the form



of degradation products from other parts of the system including the soot,



the alumina column and other TLC fractions.  The finding of 2$ on the



Chromosorb-102 means that very little BaP gets beyond the filter.  The


                     14
presence of only 3$ C  BaP in the BeP fraction is an indication of the very



good separation of these compounds by the thin layer chromatographic procedure.



Furthermore, these two compounds can be readily distinguished by UV spectrescopy.



          Table 3 also contains the results of UV analysis for total BaP.  These



data (uncorrected for analytical losses) represent direct measurement of the



compound using a calibration curve developed with BaP standard samples.  The



use of a radioactive tracer injected into the sample stream should permit



measurement of the amount of BaP in the exhaust by applying a factor for



recovery.  This is not true when the radioactive spike is added to collected



samples of exhaust emissions which contain a complex mixture of soot, unburned



fuel, and partial oxidation products.



          Even though the cold air dilution technique gave an adequate



recovery of PKA, the difficulties due to water freezing in the line and of



collecting a proportional sample, led us to look for another technique.  We



next tried a procedure recommended for use in analyzing stationary stack gas



samples, Figure 4-E.  This method utilizes a heated line followed by glass



traps containing ethanol cooled in dry ice.  The trapping went well but the



method was unsatisfactory; only 10$ of the injected BaP was recovered and



only a trace  of the BaA was  found.

-------
                                                                           19.
                                TABLE 3
            ,14.
                 ANALYSIS OF RADIOTRACER AMD UV RESULTS
                            EXPERIMENT NO. 11

                 DDAD 3-53 Engine  -  1800 rpm, 1/2 Load
    6.42 ug C  BaP Injected - 4.0 ug BaP Pre-Loaded on Chromosorb Trap
Lines & Filter
Chromosorb Trap
                        Carbon14 Balance
                       C14BaP
3.22    50.0
0.14     2.2
                     C14X(3)
                                    1.38    21.5
                   Total BaP by UV
                     Aig         %
                  3.58(1)    N/A
                  1.62       37.C
      Total
3.36    52.2
1.38    21.5
5.20
(1)  Includes C  BaP plus engine produced BaP.
                    14
(2)  Corrected for C  BaP found on trap.
                     14
(3)  Other includes C   activity measured in the following:
               BeP fraction (0.097 jag),  other TLC fractions (0.637 ,ug),
               soot (0.339/ug) and Alumina (0.305 ,ug).   Most,  if not all,
               of this activity is associated with degradation products
               of BaP.  Weights reported are calculated as  BaP based on
               radioassay.

-------
                                                                             20.
          Five other cryogenic trapping experiments were conducted using




more efficient traps; a typical system is shown in Figure 4-F.  In all these




experiments, glass wool or steel wool was used in either the first or second




trap to separate the condensed water.  In Run 14, about 43$ of BaP was




recovered though only about 17$ of BeP was recovered.  Runs 15 and 16 were




made without injecting PNA and represent direct comparisons with Runs 1




and 2.  In Run 15 (as well as Run 14) methanol was injected to prevent ice




formation.  In Run 16, no methanol was used and water vapor was collected




as snow on glass wool.  In Run 21, methanol containing ammonia was injected




to keep the condensate basic.  In Runs 15 and 16, recoveries of PNA were




lower than in Runs 1 and 2.  Run 21 resulted in higher recoveries except




for BghiP.




          In all of the cryogenic experiments, difficulties were encountered




because of the very large volumes of liquids that had to be extracted.  Glass




wool or steel wool had to be used to collect the entrained droplets of




condensate.  It was later found that recovery of PNA from either of these




packing agents is difficult.



          Two runs (18 and 19), were also similar to 1 and 2,  except that




a precooler in the sample line was used (Figure 5).  This precooler is not




intended to condense water vapor but to cool the exhaust to a  temperature at




which reactions are less likely to occur.  Again, recoveries of PNA (except




for BghiP) comparable to Runs 1 and 2 were obtained.




          Table 4 lists the recovery of BaP and BeP produced by the,engine




on a constant exhaust volume basis.  Experiments included in this table were




conducted either without BaP injection or with the use of radioactive C-^BaP




to distinguish from the engine produced material.  The amount  of engine produced

-------
                                                                          21.
                                TABLE 4
                           ENGINE PRODUCED BaP


No.
1
2
15
16
17
18
19
21
DDAD 3-53 Engine - Fuel 2-5
Test Conditions - 13-Mode Federal Cycle
Collection System (Figure No.)
Direct filtration and Chromosorb-102 (4-A)
Direct filtration and Chromosorb-102 (4-A)
Cryogenic Bath, methanol injected,
Direct filtration (4-F)
Cryogenic Bath, direct filtration (4-F)
Precooler, cryogenic trap, filter (4-F)
Precooler, direct filtration (5)
Precooler, direct filtration (5)
Precooler, cryogenic trap + filter + amraoniated


BaP
ng/M3*
75
63
20
47
51
56
58
100


BeP
ng/M3*
88
81
94
28
0
39
188
90
                         methanol injected.  (4-F)
                                        Average               58          76





                                        Std. Dev.             26          57
*Namograms per cubic meter of exhaust gas.

-------
                                                                            22.
BaP is relatively constant.  We suspect, based on these experiments, that




engine produced PNA may have behaved differently than injected PNA.  This




hypothesis is fortified by measurements of injected PNA, Table 5.  Data




for this table were estimated either from C14BaP injected or by subtracting




the average engine produced PNA as estimated in Table 4.  Only full 13-mode




cycle runs were considered.  Injected BaP recovery depended greatly on the




sampling procedure and varied from 0 to 40$.  One explanation for this




difference is that the PNA from the engine were stabilized on the soot particles




whereas the injected PNA reacted with constituents in the exhaust gases.




Presumably, some of the injected PNA was destroyed.




          If this is the case, then a collection system involving the




precooler, filter, and chromosorb trap should be adequate to collect PNA




from diesel exhaust.




          With the Project Group's concurrence, the collection system shown




in Figure 5 was adopted.  It consists of a precooler to maintain sample




temperature below 350°F (177°C), a 12" (30 cm) filter holder (Gelman Type A




filters), the Chromosorb-102 trap and a heat exchanger with condensate trap.




The 12" filter reduces the need to change filter papers during a run.





     C.  Analysis of Diesel Fuels



          A principal aspect of the CAPE-24 Project was to determine




typical PNA levels in diesel fuels.  To this end, five ASTM 1-D and sixteen




ASTM 2-D diesel fuels were obtained from around the country for analysis.

-------
                                                                         23.
                               TABLE 5



                       RECOVERY OF INJECTED MA



Exp.                                                  Percent Recovery
No.       	System   (Figure Mo.)	      BaF    BeP    BaA

 3        Direct Filtration (4-B)                     0

 4        Short exposure time of added PNA           41
               Direct Filtration (4-B)

 5        Short exposure time of added PNA                  23
               Direct Filtration (4-B)

 6        40° Condenser  (4-C)                               35


 7        65° Air Cooled Condenser (4-C)                    <7


12        40° Condenser  (4-C)                        27            16

17        Precooler, Cryogenic Trap (4-F)             3*

19        Precooler, Direct Filtration (5)           14*


21        Precooler, -90° Cryogenic Trap (4-F)       17*
^Radioactive Recovery.

-------
                     FIGURE  5
       FINALIZED  PNA COLLECTION SYSTEM
                              I30°F-300°F
                             '(54°C-I49°C
  I2in. (30cm)
  Filter
  Gel man  Type "A"
/ Precooler
. * 	 I

	 , r
i i i
i
i

Water
                     X
                     111
                                                   UJ
Proportional
  Sampler
                             Chromsorb 102
                             Trap
Condenser-40°F  (4.4°C)
                           Water  Trap

-------
                                                                             25.
          Table 6 gives the inspection data for these fuels as well as




the cities where they were obtained.  All fuels meet specifications set




forth by engine makers.




          Table 7 lists the analytical results for ENA.  The first four




columns are the principal ERA hydrocarbons that we have concentrated upon.




Concentration of individual ENA ranges over nearly three orders of




magnitude among the different fuels.  There is no correlation of ENA content




with either aromatic content or 90$ distillation temperature.




          A comparison of these results with gasoline is of interest.




Gross^ ' reports that gasolines vary in BaF content from "near zero" to




an average 500 ppb to a field maximum 3000 ppb.  The limited data in




Table 6 suggests that ASTM 2-D fuels average less BaF, and probably other




ENA, than gasoline even though the boiling range can encompass many ENA.




This fuel difference may result from the fact that diesel fuels are




essentially all non-cracked products obtained by distillation while




gasolines are principally made from products that have been catalytically




processed.  Such processes may lead to either lighter components or to




reformed components, both containing aromatics.




          Fuel 2-5 was used in all of the preliminary engine studies.




Since it is in the lowest range of ENA content, it is not desirable as




the baseline fuel for the determination of representative ENA emissions.




Because of the cost in both time and money of selecting a particular fuel




with a given ENA content, it was decided to make a blend of eight fuels




for the baseline studies.  This fuel,  2-16,  was prepared by blending eight

-------
TABLE  6
SURVEY FUEL INSPECTION DATA
Code
No.
1-1
1-2
1-3
1-4
1-5
2-1
2-2
2-3
2-4
2-5
2-6
2-7
2-8
2-9
2-10
2-11
2-12
2-13
2-14
2-15
2-16
2-17
Source
Wo. 1 Fuels - Kerosene
Philadelphia, PA
Anacortes, WA
Wood River, IL
Nederland TX
Baton Rouge, LA
No. 2 Diesel Fuels
Toledo, OH
Santa Fe Springs, CA
Port Arthur, TX
Cincinnati, OH
Philadelphia, PA
Jacksonville, FL
Denver, CO
Sullivan, MO
Waterville, ME
S. Gate, CA
Oklahoma City, OK
Portland, OR
Columbus, OH
Omaha , NE
Wheeling, WV
Composite Test Fuel
Columbus, IN
API
Gravity
42.0
43.8
44.0
41.6
41.3
34.2
36.2
34.5
35.4
33.3
34.2
34.6
33.6
33.6
34.0
36.5
36.6
35.7
35.6
35.0
36.9
35.1
Density
.8156
.8072
.8063
.8174
.8189
0.8540
0.8438
0.8524
0.8478
0.8588
0.8540
0.8520
0.8572
0.8572
0.3550
0.8422
0.8418
0.8462
0.8468
0.8499
0.8425
0.8493
Cetane
Index
47
49
51
51
50
48
52.5
46
45
42.5
47
44.5
42
41
49
50
49
46
47
46
50
48.5
FIA
Aroma tics
18.0
19.0
18.5
15.5
20.5
31.0 '
28.5
28.5
30.5
39.0
26.5
33.5
37.5
22.0
31.0
27.5
28.5
31.5
31.0
29.5
26.0
35.1
Olefins
.5
.5
1.0
.5
.5
.5
.5
.5
.5
.5
0.5
2.0
.5
.5
.5
.5
.5
.5
2.0
.5
0.5
4.0
Distillation
Sulfur
0.015
0.007
0.032
0.044
0.039
0.21
0.52
0.20
0.18
0.08
0.13
0.32
0.13
0.17
0.28
0.32
0.21
0.20
0.33
0.14
0.17
0.21
10
380
360
373
402
381
434
444
422
416
424
418
400
430
402
443
414
408
408
414
431
417
412
50
420
412
418
447
440
516
518
502
486
494
518
490
494
480
530
498
488
486
492
496
498
508
90
464
476
477
492
498
599
593
591
567
582
610
586
564
576
606
600
578
564
572
560
589
596
                                                                     to
                                                                     cn

-------
TABLE 7
Compound :
Code No.
1-1
1-2
1-3
1-4
1-5
2-1
2-2
2-3
2-4
2-5
2-6
2-7
2-8
2-9
2-10
2-11
2-12
2-13
2-14
2-15
2-16
2-17
MDL
* Equal
BaP
3
1
<1
<1
1
69
40
10
50
9
274
86
256
9
2
422
12

-------
                                                                         28.

ASTM 2-D fuels available in the Pittsburgh area.  Each supplier assured us
that they had prepared the fuel and that it was not an exchange product.
The suppliers were Amoco, Arco, Boron, Exxon, Mobil, Pennzoil, Sun, and
Texaco.  This blended fuel, which has typical inspection, has about six times
the amount of the four PlA's of interest compared to Fuel 2-5 used in the
preliminary experiments of this report.  The PWA content is in the mid-range
of the fuels surveyed.

     D.  Engine Repeatability Studies
          Four 13-mode tests were conducted to measure repeatability of the
total system.  Engine operation was identical in each test.  The baseline
fuel (2-16) and the sampling apparatus shown in Figure 5 were used. Because
injected BaP appeared to react differently than engine produced BaP that
                                                                14
remained in the exhaust, the exhaust gas was not spiked with a C   tracer.
Instead, after the exhaust sample has been collected, the solutions from
                                      14
the soot extraction were spiked with C  BaP to measure the BaP recovery
during the analytical procedure.
          Table 8 gives the results of these tests.  Note first that the
                 14                          j                     „/
recovery of the C  BaP spike is only about 2870 compared with the B5% recovery
when analyzing fuels  (Table A-2).  The 28% recovery of BaP indicates BaP and
probably other PWA react with other exhaust products even in the liquid phase.
The recovery of the three other principal PNA's are only corrected for analytical
loss  as described in Appendix A (Table A-2).  No correction for recovery was
applied to the remaining compounds because no recovery  data have been obtained
for them.

-------
                                                                            29.
                                   TABLE 8
13-MODE TEST REPEATABILITY
DDAD 3-53 Engine - Fuel 2-16
Experiment No. 22
Percent Recovery
of Radioactive BaP* 28.8
Compound - ;ug per Test
BaP, corrected 1.59
BeP 0.79
BaA -.01
BghiP 0 . 20
Chrysene 0 . 80
Pyrene 32 . 3
Phenanthrene 491
Fluoranthene 23.9
Phenanthrene Der. 445
Phenols 1280
23 24 26 Avg. Std.Dev.
(28**) 20.0 28.2 26 4.2

0.84 1.93 0.63 1.25 0.64
-.01 ^.01 0.18 0.24 0.37
•^.01 •v.Ol '.01
0.27 1.00 0.06 0.38 0.42
T.03 0.13 0.87 0.45 0.45
17.3 15.0 14.5 19.8 4.4
340 276 343 362.5 90
19.4 10.5 7.0 15.2 9.8
300 675 464 471 154
840 752 504 849 306
 ^Radioactive spike added to solutions of sample before analytical recovery is  started.
**The material recovered in this sample where BaP was expected did not contain  BaP.
  From the UV spectra of another fraction, BaP was identified.   A 28% recovery  factor
  was assumed.

-------
                                                                        30.




          The amount of each individual compound in the exhaust sample




shows a large variation.  We have no explanation for this variation.   The




collected soot using the baseline fuel was far more tarry than the soot with




Fuel 2-5.  We also experienced greater difficulty in obtaining pure fractions




during the separation because other UV-absorbing substances eluted with the




PNA of interest.  This may be an explanation of why the BaA yield is so




much lower than BaP.  In gasoline exhaust studies BaA always was found in




larger amounts than BaP.  Further work to improve the separation is planned.






     E.  Additional Engine Studies




          At the request of the Project Group, a double length test (i.e., two




13-mode cycles) was conducted.  The results appear in Table 9.  This test




should give an idea about sampling losses during sample collection.




          Recovery of the C  BaP spike was 28%, comparable to that obtained




in the replicate tests.  Because of the large amount of tar in this test,




the filter paper containing the particulates was re-extracted and the solution




separately spiked before processing.   Not a great deal of additional PEA




was recovered but the recovery for the C  BaP spike was 87.5/0 which compares




well with the 85% recovery for fuels.  This result indicates that the species




which react with PNA were extracted with the PNA in the first extraction.




          It was also observed in the double length test that BaP, BghiP,




pyrene,  chrysene, and fluoranthene were recovered at about the same con-




centration as in the single length tests.  This may indicate that these




compounds are partially lost  during sampling.  Phenanthrene, the phenanthrene




derivative and  total phenols  are recovered at about double the concentration.

-------
                                                       31.
             TABLE 9
PNA RECOVERY
Percent Recovery
of Radioactive BaP
Compound
BaP, Corrected
BeP
BaA
BghiP
Chrysene
Pyrene
Phenanthrene
Fluoranthene
Phenanthrene Der.
Phenols
IN DOUBLE LENGTH 13-M3DE CYCLE TEST
Average of 4
Single Length
Tests
26
Ug/Test
1.25
0.24
£.03
0.38
0.45
19.8
362
15.2
431
849
Double
Std. Dev. Length Test
4.2 28.1(87.5)*
ug/Test
0.64 1.21
0.37 <.01
<.01
0.42 0.78
0.45 1.00
4.4 25.9
90. 757
9.8 18.2
154 1089
306 1858
*Recovery of
aP spike from second extraction of carbon deposits.

-------
                                                                         32.






This suggests that the more stable compounds are recovered in better yield.




However, because the test variability is so high, this experiment cannot be




considered conclusive.




          A single run has been made using the smoke cycle (see Appendix C).




No difficulties were  encountered in the use of the proportional sampler or




the DATA-TRAK controller.  The filter paper was found to be quite wet with




condensed water, probably the result of the extensive idle in this cycle




(even though the idle period was reduced from 5 minutes to only 1 minute).




A very large amount of soot was collected in this test and the PNA found




was low.  Table 10 gives the PKA  found   for this test.








                               TABLE 10






                  PMA EMISSIONS FROM THE SMOKE CYCLE








               	Compound	         ug/kg Fuel




               BaP, Corrected                      1.1




               Pyrene                             39.




               Fluoranthene                       17.




               Phenanthrene                      233.




               Phenanthrene Derivative           320.




               Phenol                           2080.

-------
                                                                         33.




                                                        14
No other PNA were found.   In addition, recovery of  the C  BaP  spike amounted



to only 13.7$.  Whether the low PNA yield indicates destruction after sampling



or destruction due to the  high exhaust temperature, is not yet known.  The



yield, particularly for the lower molecular weight  species, are much lower



in the smoke cycle test than in the 13-mode tests.  These tests will be



repeated.



     F.  Comparison of Exhaust PNA and Fuel FNA



          Table 11 compares the recovery of PNA on  the basis of ug per cubic



meter of exhaust gases, ug per gallon, and ug per kg of fuel used.  These



data are average values for the four 13-mode cycles run with the baseline



fuel.  Also included in the table is the concentration of these PNA in the



fuel.  Anthracene was present in the fuel but not in the exhaust gas.



Conversely, phenanthrene was in the exhaust gas but not in the fuel.



          In spite of the  fact that the first 21 runs were made with a variety



of test procedures, a number of them can be used to calculate  an average yield



of PNA for Fuel 2-5.  They include Runs 1, 2, 15, 16, 17, 18,  19, and 21 for



which analytical data were available.  Table 12 compares the PNA recovery in



ug/test for both Fuel 2-5  and 2-16.  It is not possible to give a corrected



recovery for BaP with Fuel 2-5 since radioactive BaP, if incorporated, was



injected into the exhaust  sample stream.  The PNA in the exhaust gas from



both fuels is similar but  with differences.   The variability based on



standard deviations is comparable.  There is no evidence that variation in



sampling procedure used with Fuel 2-5 has increased the variability of



recovery.  The low yield of BaA with Fuel 2-16 may be an artifact due to the



separation procedure.  With this fuel, a considerable amount of background



material present with the  expected BaA cut.

-------
                                             34.
TABLE 11
AVERAGE

Compound
BaP
BeP
BaA
BghiP
Chrysene
Pyrene
Anthracene
Fluoranthene
Phenanthrene
Phenanthrene Der.
Phenols
PNA EMISSIONS FROM FOUR 13-MODE
DDAD 3-53 Engine
Exhaust Gas
0.158
0.031
-
0.048
0.035
2.501
-
1.88
46.1
59.8
107.2
- Fuel 2-16
Aig/Gal.
Fuel Burned
.*
4
-
7
5
349
-
253
6410
8280
14900
CYCLES

Fuel Burned
6.9
1.3
-
2.2
1.6
110
-
79
2013
2600
4680

/ug/Kg.
In Fuel
41
75
108
26
72
144
31400
869
-
-
35000

-------
                                                                     35.
                              TABLE 12
           COMPARISON OF PNA FOUND WITH FUELS  2-5 AND  2-16
                           Fuel-2-5                    Fuel 2-16
BaP
BeP
BaA
BghiP
Chry
Pyr
Phen
Fluo
Phen Der.
Phenols
Average
jog/Test
0.48
0.58
0.50
1.14
1.48
21.9
448.
ED
ND
1014.
Std.
Dev.
0.24
0.44
0.57
1.5
0.77
8.2
256.
ND
ND
266.
Average
ug/Test
1.25*
0.24
< 0.01
0.38
0.45
19.8
362.
15.2
431.
849.
Std.
Dev.
0.64
-
-
0.42
0.45
4.4
90.
9.8
154.
306.
                                      14
^Corrected for analytical loss using C   spike.

-------
                                                                         36.




          The uriburned fuel per test is calculated to be 0.102 kg, from




Table B-l.  From the measured fuel consumption (16.51 kg), the fraction




unburned fuel in the exhaust is 0.62$.  This unburned fuel contains, in




addition to the original fuel, a variety of thermal degradation products.




If synthesized ERA were not present in these degradation products, the




maximum yield of ENA would be 0.62$ of the PNA in the fuel consumed,




assuming all fuel components burn equal]Y .coll.




          Table 13 compares the PNA found in the exhaust in ug/kg of fuel




burned and the percent PM of the original fuel for Fuels 2-5 and 2-16.




Because the data on BaP for Fuel 2-16 has been corrected to account for




losses, it is probable that the yield of this compound is essentially the




same for both fuels.  In nearly every case, except anthracene, the percent




of compound present is much greater than 0.62% of the material in the




original fuel.  This in turn supports the theory that ENA are formed as




byproducts of incomplete combustion.






     G.  Comparison of Gasoline and Diesel ENA emissions




          While it is of interest to compare ENA emissions from gasoline




and diesel engines it must be remembered that the engine tests are conducted




using completely different procedures.  Data reported in Table 14 for the




gasoline engine were obtained while the vehicle was operated repetitively




in the original 7-mode Federal cycled  'Data for the diesel engine were




obtained from the 13-mode Federal diesel emission procedure.   Further,




the sampling and analytical procedures used in the two studies are totally




different.  Ignoring these differences, Table 14 suggests that the diesel




engine produces less ENA than the uncontrolled gasoline engine jn terms of




eaiissions per gallon of fuel burned.

-------
                                           37.
TABLE 13
BaP
BeP
BaA
BghiP
Pyr
Chry
Anth
Flou
Phen
Phen Der.
Phenols
COMPARISON OF
Fuel
ug/kg
Fuel Burned
2.7
3.3
2.7
6.5
121
8.2
0
0
2409
-
5600
EXHAUST PNA TO PNA IN FUEL
2-5
 of Fuel PNA
in Exhaust
30
32
18
72
27
1
0
0
CO
-
15
Fuel
Jig/kg
Fuel Burned
7
1
-
2
110
3
0
85
2015
2396
4723
2-16
% of Fuel PNA
in Exhaust
17
2
0
8
76
4
0
10
00
co
13

-------
                                                                       38.

                              TABLE 14
            PNA EMISSIONS FROM DIESEL AND GASOLINE ENGINES

                       ug/gal. of Fuel Burned
                     Uncontrolled     Controlled
                          Car         	Car	     DDAD-5-55 Engine

                                         ie.b              132
                                          13               22

                                          48                4

                                          32

                                          56                1

                                          87                7

                                         411              349

                                         383              269
Fuel BaP (ug/gal)
BaP
BeP
BaA
BghiP
Chry
Pyr
Fluo
H. Analysis of
45
77
244
119
358
231
2228
1869
Gaseous Emissions
          Part of the requirement for this project is to determine gaseous

emissions during the 13-mode cycle, and smoke emissions during the smoke

cycle.  Results are in Table 15.  Analytical data for each mode is given

in Appendix C.  These data were obtained from the four reproducibility

tests plus the one smoke cycle using the baseline fuel.

          Both gaseous and smoke emissions were below 1974 standards for

heavy duty diesel engines and are typical for this engine model.

-------
                                                       39.
             TABLE 15
     GASEOUS ENGINE EMISSIONS
   Engine DDAD-5-55 - - Fuel 2-16
                        13-Mode Cycle

                          g/BHF-Hr.
CO                           8.8
HC + WO                     10.9
       x


HC                           1.5
NO                           9.4
  x


NO                           7.5
                         Smoke Cycle



   Mode                  % Opacity



Acceleration                18



Lugging                     14



Peak                        35

-------
                                                                       40.
V.   CONCLUSIONS








     1.  An adequate sampling system has been developed for PNA




         collection.  It consists of a gas precooler,  particulate




         filter and a Chromosorb-102 trap.






     2.  Recovery of PNA from diesel exhaust may be reduced by reactions




         of PNA with other constituents in the exhaust.  Injected ENA




         shows greater variability in recovery with different sampling




         systems than does the remaining engine emitted PNA.






     3.  Twenty diesel fuels ranged widely in PNA content,  from 1 ppb




         to 422 ppb for BaP and to 466 for BaA.  These concentrations




         are low relative to gasolines which have been found to contain




         up to 3100 ppb of BaP.






     4.  There appears to be little effect due to fuels in yield of PNA




         from the DDAD-3-53 engine.

-------
                                                                         41.




VI.   REFERENCES






1.  S. K. Ray and R. Long, "Polycyclic Aromatic Hydrocarbon from Diffusion




    Flames and Diesel Engine Combustion," Combustion and Flame 8_ 139-151 (1964)






2.  C. Bailey, A. Javes, and J. Lock, "Investigation Into the Composition of




    Diesel Engine Exhausts," Fifth World Petroleum Congress, Section VI,




    Paper 13, page 209 (1959) New York, N.Y.






3.  R. Hangebrauck, D. von Lehmden, and J. Meeker, "Source of Polynuclear




    Hydrocarbons in the Atmosphere," U.S. Public Health Service Publication




    Wo. 999-AP-33, National Center for Air Pollution Control (1967).






4.  J. Colucci and C. Begeman, "The Automotive Contribution to Air Borne




    Polynuclear Aromatic Hydrocarbons in Detroit," Journal of Air Pollution




    Control Association 15, 113 (1965).






5.  C. Begeman and J. Colucci, "Benzo(a)pyrene in Gasoline Partially Persists




    in Automobile Exhaust," (letter) Science 163, 271, (1968).






6.  G. Gross, "Fourth Annual Report on Gasoline Composition and Vehicle



    Exhaust Gas Polynuclear Aromatic Content," Pages 1, 17, 18 (October 1973)




    Coordinating Research Council.






7.  "Analysis of the Odorous Compounds in Diesel Engine Exhaust." Final




    Report - June 1972 to CRC.






8.  Title 40, Part 85 "Control of Air Pollution from New Motor Vehicles and




    New Motor Vehicle Engines," Federal Register, 37_ No. 221, 24250-24320,




    November 15, 1972.

-------
                                                                        42.




                                                         APPENDIX A






                        ANALYTICAL PROCEDURES








I.   PKA HYDROCARBONS IN DIESEL FUEL






          The polynuclear aromatics (PKA) were concentrated by alumina gel




column chromotography, separated by thin layer chromotography,  and identified




by ultraviolet spectrophotometry.




          All solvents, reagents, and equipment were free of PNA and PKA




separations were performed in subdued light to reduce degradation of PNA.






     A.  FKA Concentration




          The analytical scheme used to separate the PKA hydrocarbons from




the diesel fuel is given in Figure A-l.  The concentration step is done




with a 35 x 1.9 cm glass column packed with 60 g of 2.5$ water deactivated




Woelm Alumina.  In practice, 20 grams of fuel is placed on the column and




eluted with a group of solvents.  The first 400 ml of isooctane (10) and




the 300 ml of 10 containing 20% benzene removes most of the saturates and




light aromatics.  Cut 3 was eluted with 800 ml of a 50/50 mixture of 10 and




benzene followed by 100 ml of benzene.  Cut 4, not used in this work, is




obtained by an additional 700 ml of benzene.  The four PWA analyzed for




in this investigation, benz(a)anthracene (BaA),  benzo(e)pyrene (BeP),




benzo(a)pyrene (BaP), and benzo(g,h,i)perylene (BghiP) are recovered quanti-




tatively as shown in Table A-l.




          The water content of the alumina gel is important.  The water




content of the gel was determined by the weight loss of 10 g of gel after

-------
              FIGURE A-l
DIESEL FUEL PNA SEPARATION PROCEDURE
         Fuel Sample (20 g)
                 1
       Alumina, Woelm Neutral
       2.5$ Water Deactivated
                1
4- v
Cut #1 Cut #2
^ '
Cut #3
800 ml - I0/Benzene (50/50)
400 ml - 10 300 ml - I0/Benzene (80/20) + 100 ml Benzene

J
Cellulose TLC, 1 mm
1
I
1 '
Cut #4
700 ml Benzene
\ >
Cellulose TLC, 1 mm
I
Cut A Cut B Acetylated Cellulose, 1 mm
I
Cellulose TLC, 0.5 mm
1

Acetylated Cellulose. 1 mm
i
I
t v
V TTV
I
UV



-------
                                                             44.
                  TABLE A-l
Cut
1
2
3-1
3-2
3-3
3-4
3-5
3-6
3-7
3-8
3-9
3-10
3-11
3-12
3-13
3-14
3-15
3-16
4-1
4-2
Ml.
400
300
50
50
50
50
50
50
50
50
50
40
50
50
50
50
50
50
50
50
PNA RECOVERY - ALUMINA, NEUTRAL, 2.5% WATER DEACTIVATED
10 jug Each PNA in Isooctane
Pyrene BaA BeP BaP
Eluant jug % jug % Aig % &g %
1-0
I-0/Benz.( 80/20)
I-0/Benz.( 50/50) 4.0 40
" 4.1 41
1.9 19
"
" 1.2 12
11 2.6 26
" 2.4 24 2.2 22
" 2.0 20 3.7 37
" 1.0 10 2.8 28
" 1.3 13 1.4 14
» 2.1 21
" 1.9 19
" 2.0 20
» 2.6 26
ti
ii
Benzene
Benzene
                                                                BghiP
TOTAL       10.0  100    9.2   92    10.0   100   10.0   100   10.0  1

-------
                                                                            45.
                                                            Appendix A

heating for 10 minutes at a red heat over a Bunsen burner.   Then the desired
amount of unheated gel is placed in a bottle and the necessary amount of water
added to give the desired water content.  After equilibration overnight, the
content is verified by repeating the above procedure.  The water content should
be +0.1 wt. % of the desired level.
     B.  Thin Layer Separation
          The cellulose and acetylated cellulose must be prewashed to remove
interfering ultraviolet absorbing material.  A slurry of the absorbent in
methanol is agitated in a Waring blender for 5 minutes, transferred to a
Buchner funnel and rinsed twice with more methanol before air drying.
          The cellulose thin-layer plates are prepared by adding 26 g of
cellulose to 170 ml water and mixing for 3 minutes in a Waring blender.
The solution is transferred immediately to the applicator for the preparation
of about 4 plates 20 x 20 cm and 1 mm thickness.  After air drying, the
plates are developed with 10 to remove additional interfering components,
then stored in a dessicator.
          The acetylated cellulose plates are prepared by mixing 40 g of
acetylated cellulose and 150 ml of 95$ ethanol in a Waring blender for
3 minutes.  This provides sufficient material for about 8 plates 20 x 10 cm
x 1 mm.  These plates are air dried and stored in a  dessicator.
     C.  Cellulose "Prep" Plate
          The effluent of interest, for instance cut 3, is concentrated
to approximately 0.3 ml in benzene and streaked in a very narrow band with
a 50 ,ul syringe about 2 cm from the bottom and 2 cm from the sides of the

-------
                                                                            46.
                                                                    Appendix A
plate.  Also, a spot of BaP is placed on the extreme side of the plate as
a reference compound.  The plate is then immersed, from the end opposite
the sample, in a solution of 20/o N,W-dimethylformamide (DMF) in ether, which
migrates to within 0.5 cm of the applied sample.  The plate is removed and
air dried for approximately 15 seconds, then developed in the reverse direction
with 10 as the mobile phase.
          Two cuts, A and B are removed from the cellulose "prep" plate for
further separation.  This "prep" step is usually necessary "because of the
relatively high concentration of lower molecular weight PNA making the
detection of individual bands difficult. The plate is observed under ultra-
violet light and the upper limit of Cut A marked with a pencil line between
the R  for BaP, as observed with the BaP standard, and the next higher Rf
band, which is pyrene.  The lower limit of Cut A extends down to, but not
including, the starting line.  Cut B is the material remaining on the plate
excluding the uppermost, usually yellow, band which is discarded.  These cuts
are scraped from the plate into separate Pyrex brand extraction thimbles with
a  coarse porosity disc and Soxhlet extracted for 2 hours with benzene.
     D.  Cellulose Separation
          Cut A is concentrated to about 0.3 ml benzene, whereas Cut B, due
to higher solute concentrations, is diluted to 25 ml with benzene and then
an aliquot, usually  1 ml, concentrated  to 0.3 ml.  These samples are applied
to cellulose plates  0.5 mm thick along  with a BaP standard  at the side and
developed as done previously  for the cellulose "prep" plates.  The plates are
then  examined briefly under ultraviolet light and the fluorescent bands
observed, outlined with a pencil and the Rf compared to the BaP  standard.

-------
                                                                        47.






                                                           Appendix A




The outlined bands are transferred into separate 50 ml beakers and extracted




three times with about 10 ml of hot methanol.  The methanol is filtered




through a pressure sintered glass filter into a 50 ml Erlenmeyer flask and




concentrated to approximately 0.3 ml of benzene by adding 30 ml of benzene




in 10 ml increments.




          This concentrate is then applied to 20 x 10 cm cellulose acetate




plates 1 mm thick and developed by a mobile phase consisting of ethanol,




toluene and water (13:4:4, v/v/v).  When the solvent migrates to within 1 cm




of the top, the plate is removed and the fluorescent bands outlined and




transferred directly into the pressure filter and washed with hot methanol




in three 10 ml increments.  The filter effluent is collected in 50 ml




Erlenmeyer flasks and, after adding 1 ml n-hexadecane, evaporated to 1 ml.




Three 10 ml portions of 10 are added to the flasks with evaporation after each




addition to remove all other solvents.  The 1 ml n-hexadecane is transferred




to a 1 cm ultraviolet cell and scanned for absorption in the 220-400 nm




region.  Identification and quantification is done by reference to previously




prepared absorption spectra.






     E.  Recovery




          Two experiments were done to study the recovery and repeatability




of the procedure for the fuel analysis system.  In each case, 1 ug each of BaA,




BaP, BeP, and BghiP were added to 20 g of a No.  2 fuel oil.   This fuel blend




was then put through the complete analytical separation.   The result,  given in




Table A-2,  indicates the average recovery and repeatability of the procedure




after correction for the 10 ug/kg max.,   PNA in  the original oil.   The average




recovery data have been used to correct the fuel survey results.

-------
                                                           48.
                   TABLE A-2
         PNA RECOVERY STUDY,  NO.  2 FUEL OIL
PNA Added          Amount      % Recovery      Avg. % Recovery
Benz ( a ) anthracene
Benzo(a)pyrene
Benzo(e)pyrene
Benzo(g,h,i)perylene
1 Jig
1 Jig
i ps
1 ug
72, 74
81, 88
100, 100
98, 94
73
85
100
96

-------
                                                                            49.



                                                                    Appendix A
II.  DIESEL EXHAUST GAS ANALYSIS
          The exhaust gas sample preparation steps are shown in Figure A-2.



Each of the elements of the exhaust gas sampling system are considered



separately because of the various procedures used.



     A.  Chromosorb-102



          The Chromosorb-102 is reverse flushed with 100 ml of methanol



followed by 100 ml of benzene which removes phenolic and hydrocarbon materials.



Quite often this trap contains some moisture and the pre-wash with methanol



allows a more effective subsequent wash with benzene.



     B.  Lines



          All of the connecting lines in the exhaust sampling system are



disassembled and cleaned thoroughly with a brush and benzene.



     C.  Filter Paper



          The filter housing is cleaned with a brush and benzene and the



solvent added to that obtained when the lines were washed.  The filter paper



is cut into about 1 cm squares and placed in an extraction thimble.  Then



the solutions from the Chromosorb-102 trap, lines and filter housing are



filtered through the extraction thimble into a 1-liter volumetric flask and


                      14
approximately 0.1/ug C  BaP added.  Thus, any soot particles in the rinse



solutions, are retained in the thimble along with the filter paper.  The



filter paper and soot are extracted for 16 hours with a benzene-methanol



mixture (60-40, v/v).



          The Soxhlet extract is added to the 1-liter flask and the contents



adjusted to the mark with isooctane.  Then, 25 ml is withdrawn from the flask,



diluted with 100 ml isooctane and caustic extracted three times with 0.5 W

-------
                                                                 50.
                       FIGURE A-2

EXHAUST GAS SAMPLE PREPARATION
CHROMOSORB-1-2 LINES FILTER PAPER WATER
Methanol Benzene Soxhlet Extraction Phen
and Benzene-Methanol
Benzene For 16 Hours
V >f
>
Exhaust Ga
Concentrate in
Combine Extracts
in 1 Liter Flask
1
Remove 2 5 -ml
1
Extract
With 0.5N NaOH
1 1
Hydrocarbon Caustic
Phase Phase
4
— 	 ~ 	 Phenol a ^ ~~ 	
s Hydrocarbon ^
5 ml Isooctane Total Phenols
TRAP
Is

PNA Separation

-------
                                                                       51.



                                                          Appendix A






sodium hydroxide.  The caustic phase is added to the contents of the water



trap and analyzed for total phenol.  The extracted hydrocarbon phase is




recomibined with the contents of the 1-liter flask and evaporated on a



steam bath under a stream of nitrogen to approximately 5 ml of isooctane.




     D.  ENA Concentration



          The ENA concentration scheme for exhaust gas is shown in Figure A-3




which removes interfering ultraviolet absorbing material and unwanted saturate



and aromatic components.



          The hydrocarbon concentrate in about 5 ml isooctane, obtained



by the exhaust gas sample preparation, Figure A-2, is placed on a 20 x 1.7 cm



glass column containing 15 g of alumina, Woelm neutral, deactivated with



10$ water, and eluted with 150 ml isooctane.  The isooctane eluate is allowed



to flow directly onto a second column of the same dimensions, but containing



15 g of alumina, Woelm neutral, deactivated with 2.5$ water.  After the top



column is dry and the bottom column still has a layer of isooctane over the



alumina, the bottom column is separated and eluted with 150 ml of benzene.



This fraction,which contains the ENA of interest, is concentrated to about



0.3 ml in benzene and separated by cellulose and acetylated cellulose TLC



as shown in Figure A-l and described in the fuel oil analysis section.



          This two-column separation was verified by separating a known



mixture of ENA in isooctane.  Recoveries ranged between 90 and 100$ for




benzo(a)pyrene, benzo(g,h,i)perylene and coronene.  All ENA of interest for



this investigation lie between the elution volumes of these compounds.

-------
                                                          52.
                FIGURE A-3
   EXHAUST GAS SAMPLE PNA SEPARATION
  EXHAUST. GAS HYDROCARBON CONCENTRATE
                   T
         Alumina, Woe1m Neutral

          10/o Water Deactivated

       ISOOCTANE FRACTION, 150-ml
                    i
         Alumina, Woelm Neutral

          2.5$ Water Deactivated
ISOOCTANE, 150-ml
      V
    DISCARD
BENZENE, 150-ml
 PNA CONCENTRATE

-------
                                                                           53.




                                                                   Appendix A
III.  PHENOL ANALYSIS
          A short study was undertaken to develop a method for phenol




determination in diesel fuels and exhaust gas systems.




          Two methods were investigated.  A solvent extraction gas chromato-




graphic method to determine individual phenols and a solvent extraction




colorimetric method using 4-aminoantipyrene for total phenols.




          Total phenol, after extraction with sodium hydroxide, is detected




rapidly and accurately down to 10 parts per billion by the colorimetric




method.  Individual phenols by the extraction gas chromatographic method




are detected down to one part per million for phenol, cresols and xylenols




and five parts per million for tri and tetramethyl phenols.  However, the




higher molecular weight phenols also present in diesel exhaust do not elute




from the gas chromatographic column.  Therefore, we elected to use the total




phenol procedure.




     A.  Preparation of Phenol Concentrate by Caustic Extraction




          For this program it is assumed that all phenols of interest are




extractable by aqueous sodium hydroxide.  This would include many alkyl




substituted phenols, naphthols, indanols, and possibly higher number of ring




phenols.




          A weighed sample (165 g) of diesel fuel or kerosene is extracted




three times with 0.5 N sodium hydroxide (25 ml each).  In the case of exhaust




gases, a PWA solution is obtained by solvent washing all sample lines and




traps, solvent extracting the aqueous condensate, and the soot and diluting




the combined solutions to one liter.  An aliquot (25 ml) of this solution is




then extracted with three 25 ml portions of 1.0 N sodium hydroxide.   The




alkali extracts are combined and diluted to a known volume.

-------
                                                                            54.



                                                                   Appendix A



     B.  Total Phenol Analysis



          The 4-aminoantipyrine method for total phenol was investigated.



In this procedure phenols in an aqueous solution react with 4-aminoantipyrine



at a pH of 10 +0.2 in the presence of potassium ferricyanide to form a



colored antipyrine dye.  This dye is extracted from the aqueous solution



with chloroform and its percent transmission read at 460 nm.  The concentra-



tion of the phenolic compounds is calculated as total phenol (CCH OH).
                                                               D 0


          1.  Color Development



               The caustic washings are adjusted to a pH of 10 with phosphoric



acid, and diluted to 300 milliliters with water.  Phenol I and II pillows*



containing various reagents needed for pH control and color development are



added to an aliquot of the solution further diluted to 300 ml.  The colored



dye formed at room temperature is then extracted after two minutes with



30 milliliters of chloroform into a one-inch test tube.  Percent transmission



at a wave length of 460 nm is measured by means of a Bausch & Lomb Spectronic



20 and compared with a previously prepared calibration chart to give the total



micrograms phenol present in the 300 milliliter sample taken for color



development.



          2.  Calculations



               For diesel fuel, heating oil, kerosene:



                          micrograms phenol from calibration chart

                  PPm  =          weight of sample in grams

              (total phenol)





For exhaust gas:


                         tjriginal sample vol.l

                                aliquot      ) X Micrograms from calib. chart


       (total pheno±;



* Obtained from the Hach Chemical Company, Ames, Iowa.

-------
                                                                             55.



                                                                   Appendix A






          5.   Application of Total Colorimetric Phenol Method




               This colorimetric method was evaluated for various fuels.




The results are given in Table A-3.  Each fuel was analyzed three times and




as can be seen, good agreement was obtained.  Different sample weights of




each fuel were taken to insure that the phenols were quantitavely extracted




by the caustic.




     C.  Chromatographic Analysis




          The combined caustic extracts are acidified and the phenols




extracted by three ethyl ether washings (about 40 ml each).  Organic acids




are removed from the combined ether extracts with a small volume (about




10 ml) of saturated sodium bicarbonate solution.  Finally, dissolved water




is removed from the ether by passing it through a glass column containing




anhydrous sodium sulfate.




          The ether is evaporated, under nitrogen, on a steam bath to a




volume less than one milliliter.  Orthochlorophenol (0.2 mg) was added for




use as an internal standard.  An aliquot of this volume is injected into a




gas chromatograph equipped with a capillary column.




          Figure A-4 shows a chromatogram of the phenols in an engine exhaust.



          Table A-4 shows the gas chromatograph operating conditions and




Table A-5 shows approximate retention times and flame sensitivity of various




phenols.




     D.  Methods Comparison




          The total phenol (4-aminoantipyrene) and gas Chromatographic




procedures are compared in Table A-3.  The GC Method detects considerably




less total phenol than the 4-aminoantipyrene procedure.  The likely

-------
o




o
X

o
   O
   O
   m
   z
   o
   r
   S
   m
   w
   m
   r


   m
   x
      3
      m
                   r
                                                        Ethyl Ether
                                                    •0-Chloro Phenol
                                         Phenol  654p.g
                          0-Cresol 6ug
                           2,6 Xylenol
                          P-Cresol
                         2-lsopropyl  Phenol  IIfig

                      3,5 Xylenol  4/xg
                      0-Ethyl Phenol 3u.g
                                                       M-Cresol 64/ig
                   2,3,5 Trimethyl  Phenol 9/tg
'92
                                          Unknown 196

                                          (Assuming a Flame

                                           Relative  Sensitivity

                                           of 50)

-------
                                 TABLE  A^3

                               TOTAL PHENOLS
                             4-Ami noanti pyrene
                                                                      57.
 Sample  Size
 Grams
 1.5147
 1.3052
 2.0830
#2
Heating
Oil
Total Micrograms
Phenol	
      35
      29
      46
23
22.2
22.1
1.0793
1.1156
1.5058
Diesel
Fuel
      30
      30
      40
27.8
26.9
26.6
1.0554
1.3023
1.7927
Kerosene
       3
       5
       7
 2.8
 3.8
 3.9
#2 Heating Oil
Diesel Fuel
                               GC ANALYSIS
                                                    7
                                                   19

-------
                                                   58.
                TABLE A-4
      GAS CHROMATOGRAPHIC OPERATING
     CONDITIONS FOR PHENOL ANALYSIS
Instrument
Column
Substrate
Carrier Gas
Hydrogen Flow
Air Flow
Column Temperature
Detector
Injector Temperature
Detector Temperature
Splitter Ratio
Sample Size
Attenuation
Perkin Elmer 900
150' x .02" I.D. capillary
Didecylphthalate + 10% H3P04
Helium, 12#/square inch
PSI
PS I
130°C Isothermal
Flame lonization
250°C
250°C
1:90
6 yl
As needed

-------
                                                          59.
                   TABLE A-5
               GAS CHROMATOGRAPHIC
               SEPARATION OF PHENOLS
                  Approx. minutes
Component         past injection          F1 arne  S e n s i t i v i ty (a)

o-Chlorophenol          4                     0.45
Phenol                  6                     0.61
o-Cresol                7.5                   0.66
2,6-Xylenol             7.7                   0.68
p-Cresol                9                     0.60
m-Cresol                9.5                   0.60
o-Ethyl phenol         10.5                   0.63
2,4-Xylenol            10.8                   0.60
2,5-Xylenol            10.9                   0.68
2,3-Xylenol            12.0                   0.62
p-Ethyl phenol         12.9                   0.60
iso-Propylphenol       13.0                   0.70
3,5-Xylenol            13.4                   0.69
3,4-Xylenol            16.0                   0.61
2,3,5-Trimethylphenol  22.0                   0.60
2,3,4,6-Tetra-         25.0                   0.47
  methyl phenol
(a)  W.  A.  Dietz, J.  Chromatog.  Sci.. 10, 423 (1972).

-------
                                                                           60.




                                                                 Appendix A







explanation for this discrepancy is that the phenols in diesel fuels and




exhaust gases contain naphthols and other polynuclear phenols in addition




to alkyl phenol derivatives.  These higher ring phenols do not readily




elute from the GC column.  For example, 1-naphthol had not eluted from the




column by 60 minutes, whereas tetramethylphenol elutes in only 25 minutes.




          On the basis of this limited study, and the fact that the total




phenol method takes considerably less time, it was decided to use the




total phenol 4-aminoantipyrene procedure for this program.

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                                                                                           61.
                                                                       APPENDIX B
                            DIESEL ENGINE OPERATING PROCEDURES




             The  test  procedures used  for  diesel engine operation  are

published  in the Federal Register,  Volume 37, pages  24297 and 24310-24311,

November 15, 1972.   The  description of  the  test  procedure and dynamometer

operation  are  reproduced below  for  each procedure:




      1.   Emissions  Cycle - 13-Mode  or Gaseous
           §85.974-9  Test procedures.

             The test procedures described in this
           and subsequent sections will be the test
           program to determine the conformity of
           engines with the standards set forth in
           § 85.974-1.
             (a) The test procedure begins with a
           warm engine and consists of a prescribed
           sequence of  engine operating conditions
           on an engine dynamometer with continu-
           ous examination of the exhaust gases
             (b) The test is designed to determine
           the brake-specific emissions of hydro-
           carbons, carbon monoxide and oxides of
           nitrogen  when an engine is operated
           through a cycle which consists of three
           idle modes and five power modes at each
           of two speeds which span the typical
           operating range of diesel  engines. The
           procedure requires the determination  of
           the concentration of each pollutant the
           exhaust flow  and the power output dur-
          ing each mode. The measured values are
          weighted and used to calculate the grams
          of  each  pollutant emitted per brake-
          horsepower hour.
            (c) When an engine is tested for ex-
          haust emissions or is operated for dura-
          bility testing on an engine dynamometer
          the complete engine shall be tested with
          all  standard  accessories which might
          reasonably  be expected  to  influence
          emissions to the atmosphere installed and
          functioning.
 §85.974—11   Dynamometer procedure.

  (a)  The following 13 mode cycle shall
 be followed in dynamometer operation
 tests of heavy-duty diesel engines:
Afoda No.
1
2
3
4
5 . ...
6
7
8 	
9
10
11
12.
Engine speed
Low idle
Intermediate 	
do
do , .
.. ..do 	
do
Low idle -
. Rated 	 	
do
.. ..do 	
do
	 do 	 	
Percent
load
0
2
25
60
75
100
0
100
75
60
25
2
13	 Low idle	

  (b)  During  each  mode the specified
speed  shall be held  to witJrn 50 r.p.m.
and the specified torque shall be held to
within 2 percent of the maximum torque
at the test speed. For example-, the torque
for mode 4 shall be between 48 and 52
percent of the  maximum torque meas-
ured at the intermediate speed.

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        2.   Smoke Cycle
                                                                        Appendix  B
                                                                                              62.
  § 85.874-9   Test procedures.
   The procedures described in this and
  subsequent sections will be the test pro-
  gram  to  determine the conformity of
  engines with the standard set forth in
  § 85.874-1.
   (a)  The test consists of a prescribed
  sequence of engine operating conditions
  on  an engine dynamometer with con-
  tinuous  examination  of  the  exhaust
  gases.  The test is applicable equally to
  controlled engines equipped  with means
  for  preventing,  controlling, or  elimi-
  nating smoke emissions and to uncon-
  trolled engines.
   (b) The test is designed to determine
  the opacity of smoke in exhaust emis-
  sions during those engine operating con-
  ditions which tend  to  promote smoke
  from diesel-powered vehicles.
   (c) The test procedure  begins with a
 warm engine which is then run through

 preloading and preconditioning opera-
 tions. After an idling period, the engine
 is operated through acceleration and lug-
 ging modes during which smoke emission
 measurements are made to  compare with
 the  standards. The engine is  then re-
 turned to the idle condition and the ac-
 celeration and lugging  modes  are re-
 peated. Three sequences of acceleration
 and lugging constitute the  full set  of ;
 operating conditions for smoke emission ;
 measurement.
   (d>  All  emission control systems in-
 stalled on or incorporated in a new motor
 vehicle  engine shall  be functioning dur-
 ing all  test procedures in  this subpart.


 § 85.874—11   Dynamometer operation
     cycle for smoke emission tests.
   (a) The following sequence of opera-
 tions shall be performed during engine
 dynamometer testing of smoke emissions,
 starting with the dynamometer preload-
 ing determined and the engine precondi-
 tioned (§ 85.874-16(c)).
   (1) Idle  mode.  The engine is caused
 to idle for 5 to 5.5 minutes at the manu-
 facturer's recommended low  idle speed.
 The dynamometer controls shall be set to
 provide minimum  load  by  turning the
load  switch to the  "off" position'or by
adjusting the controls to the minimum
load  position.
   (2) Acceleration mode, (i) The engine
speed shall be increased to 200±50 r.p.m.
above the manufacturer's recommended
low idle speed within 3 seconds.
   (ii) The engine shall be accelerated at
full-throttle against the inertia of the
engine and dynamometer or alternately
against a preselected dynamometer load
such that the engine speed reaches 85 to
90 percent of rated speed in 5 ±1.5, sec-
onds. This  acceleration shall be  linear
within ±100 r.p.m.
  (ill)  When  the engine  reaches  the
speed required in subdivision (ii) of this
subparagraph,  the  throttle  shall  be
moved rapidly to the closed position and
the preselected load required to perform
the acceleration  in subdivision (iv)  of
this subparagraph shall be  applied. The
engine speed  shall be  reduced to the
speed of maximum rated torque or 60
percent of  rated speed (whichever is
higher), within ±50 r.p.m. Smoke emis-
sions during this transitional mode are
not used in determining pmoke emissions
to compare  with the standard.
  (iv)  The  throttle  shall  be  moved
rapidlv to the full-throttle  position and
the engine accelerated against the pre-
selected dynamometer load such that the
engine speed reaches 95 to 100 percent
of rated speed in 10±2 seconds.
  (3) Lugging  mode,   (i)  Proceeding
from the  acceleration mode, the dyna-
mometer  controls  shall be adjusted to
permit the engine  to develop maximum
horsepower at rated speed. Smoke emis-
sions during  this transitional mode are
not used in determining smoke emissions
to compare with the standard.
  (ii) Without  changing the  throttle
position, the dynamometer controls shall
be adjusted gradually to slow the engine
to the speed  of maximum torque  or to
60 percent of rated speed, whichever is
higher.  This engine lugging operation
shall be performed smoothly over a pe-
riod of 35 ±5  seconds. The rate of slow-
ing of the engine shall be linear, within
±100 r.p.m.
  (4) Engine unloading. After  comple-
tion of the lugging  mode in subpsra-
graph (3)  (ii) of this  paragraph, the dy-
namometer and engine shall  be returned
to the idle condition described  in sub-
paragraph (1)  of this paragraph.
  (b) The procedures described in para-
graph (a) (1)  through  (4) of this fac-
tion shall be repeated until  the entire
cycle has  been run three times.

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                                                                                                                                     APPENDIX C
TABLE C-l






GASEOUS EMISSION
SUMMARY -
- 13-MODE CYCLE
DDAD 3-53 Engine - Fuel 2-16
Modal Data
Mode
1
2
3
4
5
6
7
8
9
10
11
12
13

RPM
505
1816
1822
1820
1813
1817
500
2807
2797
2801
2795
2802
505

JSP-
0.3
2.7
16.8
33.9
50.7
67.9
0.2
86.9
65.0
43.7
21.9
3.2
0.3

% Load
4.0
24.7
50.0
74.7
100.0
-
100.0
74.8
50.3
25.2
3.7
-

Bsfc
Ib/BHP-Hr
4.597
2.497
0.606
0.455
0.433
0.450
6.180
0.466
0.484
0.558
0.815
4.862
4.203

Exh. Flow
Ib/hr
241
833
837
845
843
845
240
1222
1214
1206
1205
1209
239
Bsfc
0.5472
Fuel/Air^1'
Ratio
0.0054
0.0083
0.0123
0.0186
0.0268
0.0375
0.0053
0.0343
0.0266
0.0206
0.0150
0.0130
0.0050
13-Mode Cycle
BHP
31.48
Concentration, ppm
CO
263
325
238
213
400
5788
188
1150
250
175
175
175
188
Average,
CO
8.82
K02 N0
28 139
29 128
45 222
80 407
149 633
206 857
30 146
269 843
165 618
65 382
47 230
41 181
34 136
g/BHP-Hr^3)
H02 NO( g)
1.85 7.53
HC
190
271
240
238
250
140
157
275
275
265
266
279
190
HC
1.54
Mass, g/hr.
CO
27.6
116.8
85.1
76.1
140.6
1992.1
19.5
587.5
126.7
89.1
89.9
90.4
19.5

H02
4.5
15.6
24.1
43.0
78.8
108.6
4.7
204.4
125.7
49.8
35.8
31.5
5.2

NO(2)
21.9
68.8
118.6
218.0
335.7
448.2
22.5
642.0
470.6
291.4
176.4
140.0
20.8

HC
10.0
50.2
44.7
44.9
47.0
26.7
8.6
75.3
74.5
. 71.7
72.0
75.8
10.3

Smoke,
% Opacity
0.6
0.5
0.6
0.8
2.5
14.2
0.6
4.2
2.0
1.3
1.0
0.8
0.6

(1) Includes Scavenger Air.
(2) Calculated as N02.
(3) Calculated as Specified in Federal Register.
                                                                                                                                                        CD
                                                                                                                                                        CM

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                                    TECHNICAL REPORT DATA
                             !?i ate ired >;\Hru£t\f»\s o>< the /^vcn.' before c
                              \7
    EPA-4 60/ 3_____7__4j-02_2_
     Study of Polynuclear Aromatic Hydrocarbon
     Emissions from Heavy Duty Diesel  Engines
                            5. REPORT DATE
                            	July. 1974
                                                            8. PERFORMING ORGANIZATION REPORT NO.
     R.  S.  Spindt
   PS "rCS.V.N J OR "A'.'IZATION NAME AMD ADDRbSS
     Gulf Research and Development  Co.
     P.O. Drawer 2038
     Pittsburgh, Pa.  15230
 12. SPONSORI
              GENCY NAME AND ADDRESS
     Environmental Protection Agency
     2565 Plymouth Road
     Ann Arbor, Michigan  48105
                                                            3. RECIPIENT'S ACCESS.Of*NO.
                            5. PERFORMING ORGANIZATION CODE
                                                            10. PROGRAM
                                                                             NO.
                            11 CONTRACT/GRANT NO.

                               68-01-2116
                                                            13 TYPE OF REPORT AND PERIOD COVERED
                                                                 Final
                            14. SPONSORING AGENCY CODE
 15.3UP°LF\!FNTARY NOTES
   Contract jointly funded by EPA and the Coordinating Research Council, Inc,
 16. ABSTRACT           •                                                   '  '   '       '•

        A  collection  system consisting of a precooler,  a glass fiber particulate
   filter, and a  Chromosorb-102 trap was used  to  collect PNA's from diesel  exhaust.
   PNA's are removed  from the collection system by  solvent washing and soxhlet
   extraction, concentrated,  and analyzed by thin layer chromatography.  Total
   phenols are analyzed  by the 4-amino-antipyrene procedure.

        Attempts  to validate.the sample collection  system using C^ tracers were not
   successful.  It is hypothesized that PNA's  injected  into the exhaust (i.e., tracers)
   are largely destroyed  by reactions with other  exhaust components,  while  engine-
   generated PNA's on or  within soot particles survive  these  reactions.   Radio tracers
   also indicate  that PNA-destroying reactions occur  even after collection  and extraction
   from the sample system.   Similar losses of  PNA's do  not occur with synthetic exhaust.

        The fuel  analyses showed that ASTM 1-D diesel fuels are nearly free of PNA
   compounds.  PNA levels in  ASTM 2-D diesel fuels  ranged from near zero to values that
   reach the average concentration reported in motor  gasolines.
                  DESCRIPTORS
KEY WORDS AND DOCUMb'NT ANALYSIS

              :> IDENTIFIERS OPEX E.'J'-.iJ '. ERVo
   Air Pollution
   Diesel Engines
   Polynuclear Aromatic  Hydrocarbons
   Diesel Fuels
                 PNA Analysis
                 PNA Sampling
                 Emissions
                                                                            COS AT I Field/Group
13. D'.S '':''< 3 JTIOM STATEMENT
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