EPA/540/2-89/004
   SUPERFUNDTREATABILITY
          CLEARINGHOUSE
            Document Reference:
Portier R., et al. "Field Plot Test Report, Phase III Engineering Design, Old Inger
 Superfund Site, Darrow, Louisiana." Approximately 250 pp. November 1986.
           EPA LIBRARY NUMBER:

         Superfund Treatability Clearinghouse - EUQX

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                SUPERFUND TREATABILITT CLEARINGHOUSE ABSTRACT
 Treatment Process:

 Media:

 Document Reference:
 Document Type:

 Contact:
 Site  Name:

 Location  of  Test:
Biological - Composting/Biodegradation

Soil/Sandy

Portier R., et al. "Field Plot Test Report, Phase
III Engineering Design, Old Inger Superfund Site,
Darrow, Louisiana."   Approximately 250 pp.
November 1986.

Contractor/Vendor Treatability Study

Timothy Mahon
U.S. EPA - Region VI
1445 Ross Avenue
12th Floor, Suite 1200
Dallas, TX  75202
214-655-6444

Old Inger Site, LA (NPL)

Ascension Parish, LA
BACKGROUND;  This  project  report describes  the results of biodegradation
with  indigenous microorganisms on  soils at  an oil reclamation plant.  The
site  occupied about  16 acres  including a 7.5 acre swamp.  The wastes were
oily  sludges found in lagoons, diked  tank containment areas, buried waste
areas and  in the swamp.  Wastes identified  at the site were consistent with
hazardous  materials  used at an oil reclamation plant.  Benzene,  toluene and
PAHs  were  present; no PCBs were found and very low levels of chlorinated
hydrocarbons and heavy metals were detected.  Numerous PAHs such as
naphthalene, methyl  naphthalene, anthracene and fluorene were detected in
lagoon soils and buried waste soils.  The concentrations of PAH  compounds
ranged from less than 100 ppm to approximately 5700 ppm for phenanthrene.
OPERATIONAL INFORMATION;  The purpose of the study was to determine
microorganism loading rate on the silt and  sandy clay soils.  Task I was a
screening  test to  determine the maximum toxicant loading rates.  After
selection  of the loading rate, Task II was  mesocosm tests in the laboratory
where loading, nutrients and other parameters could be controlled.  This
included evaluation  of commercially available bacterial cultures.  Field
verification studies (Task III) were conducted on special plots  set off at
the site and the plots were loaded sequentially with different waste types.
The volume of soil which was treated was not reported.  The duration of the
treatment was 35 days.  The report contains a discussion of the  mechanism
of biodegradation and an appendix showing the actual chemical reaction
pathways associated with the biodegradation of various PAH compounds.
PERFORMANCE;  Optimal loading rates of the various contaminants  were shown
to induce microbial biotransformations.  All of the compounds studied
decreased in concentration over time, but no specific correlations were
presented or discussed by the authors.  Data that was generated  only
3/89-11                                              Document Number:  EUQX

   NOTE:  Quality assurance of data may not be appropriate for all uses.

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 indicated gross trends and no contaminant destruction efficiencies were
 reported.  Also there was no analysis for toxic intermediates in this study
 and the authors suggested that toxic intermediate production needed to be
 evaluated further.   No specific QA/QC procedures were reported.   The
 authors state that  microbial degradation and detoxification of the site is
 scientifically verifiable and economically feasible although no  discussion
 of the economics was contained in the study.  Post closure monitoring of
 soils and leachate  from the site was recommended for 30 years.

 CONTAMINANTS:

 Analytical data is  provided in the treatability study report.  The
 breakdown of the contaminants by treatability group is:

 Treatability Group              CAS Number        Contaminants

 W08-Polynuclear Aromatic       120-12-7           Anthracene
                                91-20-3           Naphthalene
                                85-01-8           Phenanthrene
                                208-96-8           Acenaphthylene
                                86-73-7           Fluorene
                                206-44-0           Fluoranthene
3/89-11                                              Document  Number:   EUQX
   NOTE:  Quality assurance of data may not be appropriate  for all uses.

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                                             -  7  >'~J<1-   i
                           November 18, 1986
Mr. William B. DeVille                        DWMS  Project  No.  6752-0101
Administrator                                    GDC  Project  No.  85-501
Inactive and Abandoned Sites Division       DEQ  Contract  No. 25202-85-01
Louisiana Department of                            Correspondence  No.  84
Environmental Quality
P. 0. Box 44307
Baton Rouge, LA  70804
                              Transmittal
                         Field Plot Test Report
                        Old Inger Superfund Site
                           Parrow. Louisiana
Dear Mr. DeVille:
IT Corporation is herein transmitting the report  of  the  field  plot  tests
as prepared by LSU.  LSU was subcontracted by IT  to  review data from the
field  tests  and other  tests  performed by West  Paine Laboratories  and
Robert S.  Ke,rr Environmental Research  Laboratory.    The  review of  the
data was  to substantiate other  facts  that indicated biodegradation  as
the most feasible  and  environmentally  sound  remedial  action alternative
at the site.

In  summary,   LSU   made  positive  input  in  public  attitudes   about
biodegradation.  The  reports concludes that this alternative  will  work
if properly  managed.    IT/GDC did  not  wish to rewrite  the report  in  a
more  amiable  (as  far  as  IT/GDC  is  concerned)  format,  however  LSU's
conclusions are supported and favorable to the overall project.
Yours Truly,
Mark L. Morgan, P. E.
Senior Project Manager

MLM:jlc

Attachment

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                                                          K
Project Report:    Old Inger Abandoned Hazardous Waste Site,
                  Darrow, Louisiana
                  In  Vitro and In Situ Investigations
                  La DEQ Contract No. 25202-85-01
                  IT.  Project No. 435062
                             Submitted to:

                           Mr. Mark Morgan
                   IT Corporation, Baton Rouge, LA
                            Submitted by:

                       Ralph  Portier, Ph.D.
                       Martina Bianchini, M.S
                       Paul Templet, Ph.D.
                       Debra  McMillin,  M.S.
                       Charles Henry,  B.Sc.

                  Institute for Environmental  Studies,
              Louisiana State University,  Baton Rouge, LA
            I.E.S. Technical Report Number: 86-09-20-6103

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                             ACKNOWLEDGEMENTS
       Mrs Susan Cange and Ms Debra Saxton, project engineers of IT Corporation and
GDC Inc., respectively, coordinated the collection and transportation of the field samples
to our laboratory. It must be acknowledged, that IT Corporation as prime contractor of this
project was responsible for the preparation, application and subsequent sampling in all
field studies and related activities conducted. Their contribution is greatly appreciated.

       Mr. John Matthews, research biologist at US- EPA Robert S. Kerr
Environmental Research Laboratory, Ada, OK provided Microtox™ support and
Microtox™ data sets. His interpretation of these data sets and his input into microbiological
investigations is recognized and appreciated.

       This project was supported by funds from LSU contract # 135-20- 6103 with
funds provided by IT Corporation through a general technical service contract with the
Louisiana Department of Environmental Quality (DEQ) (Hazardous and Abandoned Waste
Sites, Mr. William Deville, Director). IT Project No. 435062. DEQ Contract 25202-85-01

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                            TABLE OF CONTENTS


                                                                     PAGE


List of Figures  	  vi

List of Tables 	   v

I. Introduction 	   1

n. Site Characterization   	   5
             1. Location 	   5
             2. History	   2
             3. Waste Identification	   2
             4. Site Soil Profile	   2

HI. Materials and Methods	   6

      A. Experimental Design	   6
             1. General 	   6
             2. Screening Tests	'...,.   6
             3. Mesocosm Tests 	   8
               a. Addition of Commercial Inoculum	   8
               b. Sequential Re-loading of 4% and 2.5% Waste Mesocosms	   9
             4. Field Verification Studies	   9

      B. Field Sample Collection	   11

      C. Biological Methods	   11
             1. Microbial Diversity	   11
             2. Microbial Adenosine 5* Triphosphate (ATP)	   12
             3. Microtox™  	   12
             4. Plant Biomass Determination	   13

      D. Analytical Methods	   15
             1. Soil/Waste Chromatographic Analysis	   15
             2. High Performance Liquid Chromatography (HPLC)	   15
             3. Soil Moisture and pH	   16

      IV. Results	   17

      A. Pure Waste Analysis	   17

      B. Screening Tests 	   17
                                     in

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                       TABLE OF CONTENTS (CONTD)
       C Mesocosm Tests 	  24
             l.Chromatographic Analysis	  24
             2. Microbial ATP	  29
             3. Microbial Diversity	  29
             4. Microtox™	  34
             5. Commercial Inoculum Test	  36
             6. Sequential Re-loading of 4% and 2.5 % Waste Mesocosms	  40
             7. High Performance Liquid Chromatography (HPLC)	  40
             8. Soil Moisture and pH	  41

       D. Field Verification Studies 	  46
             1. Chromatographic Analysis	  46
             2. Microbial ATP	  52
             3. Microbial Diversity	  57
             4. Microtox™ and TOC Analysis	'.	  61
             5. Soil Moisture and pEL	  63
             6. Plant Biomass Determination	  68

V.  Discussion 	  71

VI. Conclusions and Recommendations 	  78

VH. Bibliography  	  81

VIE. Appendices 	  87
                                    ill

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                              LIST OF FIGURES
FIGURE	TITLE	   PAGE
1     Chemical structures of some common aromatic hydrocarbons	3
2     Experimental  design	7
3     Mesocosm  identification  	
4     Microbial ATP profile of screening test mesocosms	21
5     Microbial diversity of screening  test  mesocosms	22
6     Microtox™ analysis of WSF of screening test mesocosm soil/waste loading	23
7     GC/FBD analysis for 4% lagoon waste, F-2 fraction over a 35 day time frame	25
8     GC/FID analysis for 4% lagoon waste, F-l fraction over a 35 day time frame	26
9     GC/FID analysis for 2.5% lagoon waste, F-2 fraction over a 35 day time frame	21
10    Microbial ATP profiles of 4% lagoon mesocosms plus Cl, day 7-35	30
11    Microbial ATP profiles of 2% lagoon mesocosms plus Cl, day 2-35	31
12    Microbial diversity of mesocosms plus Cl, day  7-35	 32
13    Microbial diversity of mesocosms day 28-84	33
14    Microtox™  toxicity  results of mesocosms	 35
15    Total ion chromatograms of mesocosms with commercial inoculum.	37
16    Degradation rates for commercial inoculum as compared to 4% adapted inoculum,.. 38
17    Degradation rates for commercial inoculum as compared to 2.5% adapted inoculum. 39
18    GC/FID analysis for group I and group n over a 25 day time frame	47
19    GC/FID analysis for group I, F-2 fractions, day 65-156	48
20    GC/FID analysis for group n, F-2 fractions, day 46-156	49
21    GC/FID analysis for PL50"-20"	51
22    GC/FID analysis for alternate locations of PL5,0"-6"	53
23    Microbial ATP profiles of field plots, day 0-37	54
24    Microbial ATP profiles of field plots, day 51-113	55
25    Microbial ATP profiles of field plots, day 156-184	56
26    Microbial diversity of field plots, day 23-58	58
27    Microbial diversity of field plots, day 102-141	59
28    Microbial diversity of field plots, day 170-184	60
29    Microtox™ toxicity results of field samples, 0"-6"	62
30    TOC analysis for day 149	.64
31    TOC analysis for day 184	.65
32    Plant biomass determination of field plots	69
                                        IV

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                               LIST OF TABLES
TABLE                       TITLE                                     PAGE

1       Schedule of field plot activities	16.
2       Waste statistics	24
3       Primary F-2 constituents in CERCLA waste site	26
4       Primary F-l constituents in CERCLA waste site	27
5       Total Organic (TOC) analysis for laboratory mesocosms	28
6       F-2 fraction; 2.5% lagoon waste	36
7       % Moisture of laboratory mesocosms	50
8       pH Profiles of laboratory mesocosms	52
9       % Moisture of fieldplots	75
10      pH Profiles of field plots	76

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/. Introduction
       This study reports the results of a multi task project  for achieving biological closure
of an abandoned hazardous waste site situated on the Mississippi River. Biological closure
is a method which employs microorganisms to detoxify  chemical compounds present in
hazardous waste sites.
       The study was divided into three tasks, namely task I (screening tests), task It
(mesocosm tests) and task HI (field verification studies). In all three tasks waste materials
from the site were evaluated for biodegradative potential and acute toxicity of leachate. The
validity of the task I tests for establishing biotransformation/biodegradation kinetics and
leachate toxicity were documented in subsequent task n laboratory mesocosm studies and
task HI field studies.
       The selected cleanup procedure for the site was onsite biodegradatipnjiirougjhland
treatment orjn situ biological treatment (ISBT). This method was chosen because it is
consistent with the National Contingency Plan in that it provides the lowest cost alternative
while providing  a permanent remedy for an existing or potential threat to public health and
the environment Onsite biodegradation through land treatment (ISBT) was selected as the
least expensive alternative and most applicable to the site. This technique consists of
spreading and mixing the oily sludges over land^providing nutrient supplements,
optimizing the necessary growth conditions  for the microbial growth, and promoting the
decomposition of hydrocarbon components by the indigenous microbiota (8). Such
manipulation of the site, "while favoring microbial growth and promoting the degradation
of the toxicants, also reduces the potential threat of ground water pollution. A literature
review explaining microbial hydrocarbon degradation processes is presented in Appendix
A.
       According to RCRA regulations, after successful completion of task I, n and ffl
studies, soils and wastewater must still be biologically monitored to prepare the site for the
final remediation consisting of the establishment of a permanent vegetative cover and post-

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Louisiana Environmental Control Commission in June, 1981, that the site was
abandoned.  Appendix A shows a map of the site and the nature of the contaminated
areas. The site occupies about sixteen acres, including a 7.5 acre swamp. The wastes are
found in lagoons I and n, the diked tank containment area, the buried waste area and the
swamp. Contamination is observed to a depth of three to five feet in the areas of the
lagoon I and filled portion of the swamp. Swamp  sediments are less contaminated.
Lagoon I occupies an area of 0.7 acre. At least 50  partially filled 55 gallon waste drums
are located in the northeastern comer of that lagoon and in the filled southeastern comer
of the swamp (17).
3. Waste Identification
       The wastes identified at the site were consistent with the nature of the oil
reclamation plant They were mixtures of refinery oils, motor oils, and lubricating  o'ils. As
is typical of waste oils, hazardous priority pollutants such asJbenzene, toluene and PAHs
were present (16). No PCB's were found; very low levels of chlorinated hydrocarbons and
low levels of heavy metals were found. The waste statistics  are shown in Table 2.
4. Site Soil Profile
The site soil consists predominantly of silty and sandy clays, silts and fine sands, to a
depth of about 115 to 125 feet (16). Below this is  a substratum silty sand, a potential water
supply source. The average vertical and horizontal permeability were both about Ix 10~5
cm/ sec (10 ft/ year). Groundwater was encountered generally ata depth of six to twelve
feet, however rising to within a few feet of the ground surface (16). Trace amounts of
some hazardous compounds had migrated vertically through the site soils to depths of 20
feet or more. Trace amounts (parts per billion) were found in the groundwater at the site to
a depth of 75 feet (17). The potential for continued vertical and horizontal migration of
hazardous compounds exists.

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FIG.  1:     Chemical structures of some common aromatic hydrocarbons.
      Alkyl Benzene
            Naphthalene
      Anthracene
Acenapthene
Phenanthrene
      Pyrene
      Chiysene
      Benzo[a]pyrene

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TABLE 2:
   Waste  Statistics
Type
Maior Problem Area
       Jiuantitv
Oily sludges
Heavily cont. soil

slightly cont. soil
Heavily cont. fluid

slightly cont water
cont. wood
slightly conL
groundwater
Total:
waste oils, sludges and
heavily (visibly) contaminated
soils and sediments
   Lagoon 1
   Lagoon 1
   Buried waste area
   swamp, S.E. swamp
   all areas
   Lagoon 1
   tanks
   swamp
   swamp
   shallow silt lens
  7,600 CY
  34,ooo CY

 70,000 CY
2.5 million gallons

7.5 million gallons
5000 tons
10 million gallons

51.500CY

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closure monitoring. The cleanup time required using this method (ISBT) takes the longest
time of all alternatives, but provides a permanent solution to this public health and
environmental threat. Risks would be posed by using other techniques. For example,
offsite RCRA landfill  would not only pose a transportation problem, but just place the
sludges in long term storage, where they could pose a potential threat and demand future
remedial actions.
II. Site Chracterization
1. Location
       The hazardous waste site investigated was the "Old Inger" site. This is a CERCLA
site (Comprehensive Environmental Response, Compensation and Liability Act of 1980)
located on die East bank of the Mississippi River. The site lies about 4.5 miles north of
Darrow, LA, in Ascension Parish between Highway 75 and the Mississippi River. In April
1982, the site was designated  as Louisiana's number one priority  forSuperfund
assistance. It scored highest out of the five qualifying sites in Louisiana, with 48.98 on the
EPA Superfund list
2. History
       Old Inger is an abandoned oil reclamation facility that was operated between
1967 and 1978. During these times, waste oils were brought to the site by barge and
truck. The oil was re-processed in  the larger of the two cracking towers by heating the
heavier oils to increase their viscosity and mixing them with used lubrication oils, light
oils and spent solvents (16). Finalproducts were transported from the facility by truck.
As part of the plant operation, sludges were stored in a large, open lagoon and/or buried
in a shallow pit; some wastes were spilled into the adjacent swamp. In March 1978, a
large spill contaminated a total of 16 acres of the surrounding area. This spill was
associated with the unloading of used oil from a barge in the Mississippi River. A shut
off valve failure or human error led to overtopping a tank and containment area (16).
Failure by the owner to clean up  the site resulted in the formal declaration by the

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///. Materials and Methods
A. Experimental Design
1. General
       The study was comprised of three tasks, namely task I, task n, and task EL
Task I consisted of a series of screening tests with the objective of determining the
maximum toxicant loading rate that would not stress the microorganisms. These
screening tests involved the determination of optimal toxicant loading rates for the
CERCLA site on the basis of both indigenous microbial population performance and
acute toxicity by leachate analysis. The major correlative aspects of task I,n and ffl, are
depicted in Fig.2.
2. Sreening Tests
       Screening tests consisted of a series of experimental mesocosms with a mixture
of Mississippi river silt and top soil (sandy clay). Soil mixtures type I and type n
consisted of one part river silt and two parts top soil, and  two parts river silt and one
part top soil, respectively.  The soils were collected from Johnson's Pit located
approximately 11 miles from the site and air dried prior to analyzation. The soils were
passed through a no. 10 sieve (Soiltest, Inc.) to remove larger particles and plant
residues, and also to render them more homogenous. Soil type n was found to be
unsuitable because of its lower £€59,  detected by EPA research laboratory in Ada, OK.
The EC 50 was defined as the Vol % distilled, deionized water (DW) extract effecting
50% decrease in bacterial bioluminescence during a 5 min test period. It represented a
relative measure of acute toxic characteristics of water soluble constituents contained in
the soil/waste matrix.Soil type I, therefore, was selected for the course of the
experiment The loading rates of the wastes mixed with soil type I were 2% oil and
grease (O&G), 4% O&G, 8% O&G and 12% O&G by weight, based on standard oil
and grease test) for both lagoon and buried wastes. No nutrient amendment was added.
The microbial activity was evaluated 2 hours after mixing,  and re-evaluated over a

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FIG. 2
Experimental design
            SREENING TEST
   LITERATURE
   SUBYEY
   COMPUTER
   BASES
   FATE/CFFECT KINETICS
   TRANSPORT STUDIES
                                    MESOCOSM TEST
                                 M1CRQBIAL
                                 ATP
                                           SOIL:::::::
                                           ENZYMOLOGY
                                           FATE: ::::
                                           ANALYSIS

                                           MICROB-JAL
                                           DIYCRSITY

                                           BltDACCUM
                                           ULATIOM '•
                                          :POTE:NTJAIS
                                          : HEAVY ::::
                                           METALS
                               KINETIC MODELS
                                              LEACHATE/
                                              LOADING
                                              RATE
                                              RATIOS

                                              SOIL/
                                              WATER
                                              SATURATION
                                              EXPERIMENTS
                                               SURFACE
                                               RUNOFF
                                               TESTS
                                                         MIGRATION
                                                         MODELS
9D
O
   TOXICOLOGY/NET DETOXIFICATION ASSESSMENT
                                         BASE
               ^^^^

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period of 17 days after mixing. The optimal loading rates for each waste type were
indicated by the highest levels of microbial activity.
3. Mesocosm  Tests
       After determination and selection of an acceptable loading rate, task n studies were
carried out The objective was to evaluate microbial biotransformation and biodegradation
of key pollutants in the laboratory, under controlled parameters, such as pH and moisture,
and to determine the optimal re-application schedule for the subsequent field verification
studies. A battery of 21 mesocosms was set up in the laboratory to evaluate
biotransformation processes under the selected loading rates. All mesocosms were set up in
triplicate. CS1, CS2 and CS3 were controls without toxicant loadings.  1L4,2L4 and 3L4
were test replicates, loaded with 4% lagoon waste. 1B4,2B4 and 3B4', also test replicates,
were loaded with 4% buried waste. 1L2, 2L2 and 3L2; 1B2,2B2 and 3B2 were test
replicates loaded with 2.5 % waste for lagoon and buried waste, respectively. Mesocosm
identifications with the soil/ waste concentrations are given in Fig.3. Along with the waste
loadings, each mesocosm (4.5 kg soil mixture type I) received nutrient amendments
consisting of 4.0  g KH2?O4) 2.0 g K2HPO4,1.0 g MgSO4 and 0.5 g KNO3. Nutrient
amendments were incorporated very thoroughly into the soil mixture, together with the
waste application, at day zero. A second nutrient amendment consisting of 2.0 g KH2PC>4,
2.0 g K2HPO4,1.0 g MgSC>4 and 0 J g KNO3, followed on day 27. The moisture was
also adjusted on day 27. Since microbial oxidation of PAH requires molecular oxygen, the
whole contents of the mesocosms were re-mixed weekly, to guarantee oxygenated
conditions.
a. Addition ofCommerial Inoculum
       In addition to investigations of the biotransformation processes by indigenous
microflora, it was of special interest to evaluate the use of a commercially available blend of
bacterial cultures. These commercial  cultures are marketed for their known ability to
degrade polynuclear aromatics. Their application is referred to by the supplier as

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"bioaugmentation". The inoculum used in the experiment was purchased from Microbe
Masters, Inc., Baton Rouge, LA. For further product data and application instructions, see
Appendix B. Two of the 21 mesocosms were inoculated with the commercial bacterial
blend at the suggested rate of 0.01 lb/ 4.5 kg soil mixture and labelled Cl and C2. Cl
contained lagoon waste at 4% load plus the inoculum. C2 mesocosm contained buried
waste at 4% load plus the inoculum. These mesocosms were inoculated at day zero.
b. Sequential Re-loading of 4% and 2 5% Waste Mesocosms
       Another aspect of the design consisted of sequential reloading the 4% and 2.5 %
mesocosms after the initial wastes had been sustantially degraded The study  of the effects
of repeated waste applications  would allow extrapolations for the task IH field verification
studies. At day 35, the mesocosms labeled 1L4,2L4,3L4,1L2,2L2,3L2,
1B4,2B4,3B4,1B2,2B2 and 3B2 were reloaded with the same waste concentrations as
initially received.
4. Field Verification Studies
        In task m, the field verification studies, 5 plots of 12' x 50' x2' were set off at the
site, filled with soil type I and loaded sequentially with the two waste types. Plot one
(PL1) served as a control plot (no toxicant loading). Plots two and three (PL2, PL3) were
classified as group I and received only buried waste loadings. Plots four and five (PL4,
PL5) were classified as group n and received alternate waste applications of buried and
lagoon waste at loading rates of 4% and 2.5% O & G. Periodic tillage and fertilization of
all the plots (control included) were carried out to optimize the biodegradation. The
objectives of the field plot activities  were to: 1) determine the fate of the waste in the
ecological system:  2) to determine whether it was degraded, immobilized or transformed;
and 3) whether hazardous constituents were migrating below the treatment zone. A
complete schedule of the field plot activities is given in Table 1. All field applications and
collections were performed by and/or under the supervision of the prime contractor on this
project, IT Corporation. They were started in Oct. 1986 and completed in April 1986.

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TABLE 1:
                      SCHEDULE OF FIELD PLOT ACTIVITIES
DAY
0


14
23
46
51
59
102
DATE
10/08/85
10/09/85
10/10/85
10/25/85
11/04/85
11/25/85
(day 30)
12/02/85
12/09/85
01/22/86
(day 60)
WASTE APPLICATION
4% Buried (129 gaUplot or 2.3 drums/plot)
4% lagoon (339 galVplot or 6 drums/plot)



2% lagoon (170 gaUplot or 3 drums/plot)


2.5% buried (80.49 gal./plot or 1.5 drums/plot)
PLOT
PL2, PL3
PL4.PL5



PL4.PL5


PL2, PL3
PL4, PL5
ACTIVITIES


Tilling of PL4.PL5
Fertilization (5 Ibs/plot
10-5-5) and tilling of all f
plots
Raking of surface and
removing of crust of all p




156
03/18/86
2.5% buried (1.5 drums/plot)                 PL4, PL5
Tilling of PL4.PL5

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B. Field Sample Collection
       Soil samples and soil cores were collected at random on each plot with a Shelby
tube sampler. Six random soil samples were composited to form one sample. Records
were kept to to make sure the same areas were not resampled. Holes were plugged with
fresh soil. Microbial samples were taken once a week to a depth of six inches for general
biological monitoring. The soil core samples were taken after the 2nd and 4th waste
loading, from a depth of 0-24 inches and analyzed in 6-inch increments. Sampling days
were field adjusted according to the soil moisture contents. No samples were taken after
heavy rainfalls. All the microbiological methods were performed on fresh soil samples
(stored on ice dun" - transportation to the laboratory). Analysis was within 3 h after
sampling.
C. Biological Methods
       The following standard microbial activity estimations, bioassays and analytical
methods were conducted for all tasks of the research project:
1. Microbial Diversity
       The Standard Plate Count Method (SPC) is a direct quantitative measurement of the
viable aerobic and facultative anaeobic microflora. Four general groups of
microorganisms, i.e. bacteria, actinomycetes, yeasts and filamentous fungi were
enumerated using colony forming units (CPU) and SPC. 1.0 g of soil (wet weight) was
weighed into 99 ml sterile, distilled and deionized water. Further dilutions were made in
phosphate buffer, as described in the EPA Manual 600/8-78-017.(20). For the
enumeration of bacteria and actinomycetes, replicate 1.0-ml aliquots were inoculated on
Jensens agar medium (31) supplemented with  40 Jig/ml cycloheximidine (Sigma) to inhibit
growth of filamentous fungi.  All plates were incubated for 4 d at 30 C. Bacteria and
actinomycetes were counted on an automatic colony counter  (Biotran m, New Brunswick
Scientific).The spot size of the monitor was set on  0.2 mm throughout all measurements.
Filamentous fungi and yeasts were enumerated on Martins agar medium (41), with 30
                                          11

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jig/ml streptomycin (Sigma) and 30|il/ml chlorotetracycline (Sigma)  to retard bacterial
growth. All plates were incubated for 4 d at 30 C, and were enumerated manually.
2. Adenosine 5'Triphosphate (ATP)
       A modification of the adenosine 5' triphosphate (ATP) assay as advanced by
Holm-Hansen and Booth (28), and further presented by Van de Werf .Verstraete (62) and
Karl (34), was used for determination of microbial biomass. 1.0 g aliquots from all
mesocosms (wet weight) were transferred into dilution bottles containing 99 ml sterile
distilled and deionized water, homogenized on a homogenizer (Janke&Kunkel Ultra Turrax
SD 45) for 45 sec. A 100 pi aliquot of that suspension was then transferred to a 3 ml
plastic vial. The vial was inserted into a Lumac 3M Biocounter (Biocounter M 2010,
Lumac Systems, USA),  100 pi of buffer and 100 pi Nucleotide releasing agent for
bacterial cells (NRB reagent) were added. ATP was released from microbial cells by
adding NRB to a 100 jil sample. After application of this reagent, ATP was measured
using the following reactions:
Luciferin + Luciferase +ATP -Mg++_> (Luciferin-Luciferase-AMP) + ppj
(Luciferin-Luciferase-AMP)-O2-> Decarboxyluciferin +Luciferase +CO2+ AMP + light
       Following the injection of 100 pi of a Luciferin luciferase solution (Lumit, Lumac
systems) into the vial, light outputs expressed as relative light units  (RLU) were
determined over a 10 sec integration period. Relative light units were expressed as
|ig ATP/g dry net weight using standardized  10 pi aliquots of a known ATP standard.
Quench corrections for each environmental sample were established with 100 |il aliquots of
ATP standard /buffer added in place of the 100 pi buffer addition.
3. Microtox™
       Acute toxicity assessment of waste/soil water soluble fractions (WSF) was
determined following procedures described in the EPA Permit Guidance Manual on
Hazardous Waste Land Treatment Demonstrations (19). A maximum acceptable initial
loading rate for task I studies was determined by calculating the EC50 and 95% confidence
                                          12

-------
intervals for the raw waste WSF. The lower EC5Q limit for the confidence interval provides
the maximum loading rate for waste/ soil toxicity testing. In this case, the EC 50  is the
volume % of the distilled deionized water (DW) extract effecting a 50% decrease in
bioluminescence of the luminescent marine bacterium, Photobacterium phosphoreum
during a 5 min test period. The DW extract was obtained by shaking 400 ml DW + 100 g
sample for 24 h.
       Mesocosm and field analyses are reported using LL5 values, defined as the five
minute mean light loss for the combined full strength (100%) and half strength (50%)
dilutions of the DW extract. T.T.5 values combine the measured dose-response with the
slope of the dose-response curve to provide a relative measure of the acute impact of the
WSF of the waste-soil matrix for comparative purposes when a quantitative EC50 cannot be
established. Results are reported +- relative standard deviation calculated for all test results
falling within a given LL5 range. A toxic effect is observed (OTE) when definite loss of
light is more than  20% (LL5 > 20%). LL5 values of 20-25% are considered to be in the
transition area and must be interpreted with care (Matthews, personnel communication).
Stimulation of bioluminescence was reported where TI.5 values were £ -5%. All samples
were analyzed on  a Microtox model 2055 Toxicity Analyzer (Beckman Instruments, Inc.,
Carlsbad,CA). All reagents and test bacteria were supplied by the manufacturer. Microtox
analysis for this project were conducted by EPA personnel at the Kerr McGee Laboratories,
ADA.OK.
4. Plant Biomass Determination
       Four months after the last activities at the field plots were carried out, a sampling of
the above ground plant biomass of the individual plots was sampled. The amount of plant
biomass was indicative of phytotoxic residual levels of oil sludges incorporated into the soil
earlier. From each plot, within a randomly selected surface of 1m x 1 m, above-ground
plant matter was collected, transported to the laboratory and dried in a drying oven at a
                                         13

-------
temperature of 100 C for three days. The amount of dry weight plant biomass/m2 was
determined by weighing the plant matter after drying.
                                          14

-------
D.  Analytical Methods
1. Soil/waste Chromatographic Analysis
       The principal analysis performed on the soil/waste samples involved determination
of aliphatic (F-l) and aromatic hydrocarbons (F-2). Replicate samples of the waste/soil
mixture were used to form a composite and 2.0 g aliquots of that composite were
subsampled and extracted with 10.0 ml of dichloromethane (DCM). A portion (100}il) of
the extract was placed on a silica gel column for sample cleanup and fractionation. The
column was eluted, first with hexane to remove the trapped aliphatics (F-l), and then with
30% DCM in hexane to elute the polynuclear aromatics (PAH). The two liquid fractions
were analyzed in a gas chromatograph with a flame ionization detector (GC/FID; Hewlett
Packard 5890, equipped with a 50 m x .32 mm DB-5 phenyl methyl silicone capillary
column). Each compound peak was quantitated and identified by GC/MS (Hewlett Packard
5970B, directly interfaced with another HP 5890 GC). The identities of several unknowns
were elucidated in this manner. Data of mesocosm samples containing the commercial
inoculum was presented by GC/MS total ion chromatograms (TIC) using a 25 m x 0.17|im
film 5% phenyl methyl silicone column (0.32 mm ID). Oven temperature started at 50 C for
3 min and increased by 8 degrees C per min to a final temperature of 280 C held for 4 min.
A complete protocol for GC determination of the Fl and F2 constituents is presented in the
Federal Register/Vol. 49. (22).
2. High Performance Liquid Chromatography (HPLC)
       Aliquots of the soil/waste mixture (2.0 g wet weight) were weighed, placed into
clean 25 ml scintillation vials. 20.0 ml DCM were added. After mixing by hand-shaking,
the vials were sonicated for 12 min and stored over night at 4 C to let the particulates settle.
With a transfer pipet, 3.0 ml liquid were transferred from the vial into a syringe barrel,
attached to a Millex-SR 0.5JJ. non-sterile PTFE filter. This solution was pushed through the
filter into a 3 ml autosampler vial, capped with a septum cap using a  teflon septum. A 10
jil aliquot of that extraction was injected into a Waters HPLC 840 System. Integration  of
                                         15

-------
area counts was based on a 3-pt least-squares standard curve, not forced through zero. The
detector used was a fluorescence detector (FL, gain : 2), using an excitation wavelength of
254 nm, and emission wavelength of 375 nm. The column used was a Waters Radial
Compression Separation System (RCSS) column, 5 mm internal diameter (I.D.) x 10 cm;
10 Jim particle size. Separation of PNA's was achieved using a 40 min. water/acetonitrile
(ACN) linear gradient beginning at 35% ACN and ending at 100% ACN, and held for two
min. before reequilibration.
3. Moisture andpH
       Analyses of soil moisture and pH were conducted weekly for a total period of 84
days for the task II studies and 184 days for the task HI studies.
The  % soil moisture was determined over the whole course of the experiment by weighing
out approximately 20 g of  wet soil and drying it in a Blue M constant temperature cabinet
at 120 C for 24 h. The % moisture was determined using the following formula:

                                        Wet weight - dry weight   x  100
                                              dry weight
                                         16

-------
 IV. Results
 A. Pure Waste Analysis
       Tables 3 and 4 present data on the analysis of the "pure wastes", classified as
 "buried waste"  and "lagoon waste" and the river silt (control). About 24 PNA's were
 identified (F-2 fraction) (Table 3) and 22 aliphatic hydrocarbons (F-l fraction) (Table 4).
 Quantitation was by external standard GC in all cases. Some analyses were semi-
 quantitative due to problems in obtaining accurate external standards  and the large
 dilution factors necessary to prevent GC detector saturation. Detection limit for both
 fractions was 100 ppm due to the large dilution factors and the lower response factors of
 the higher molecular weight components. The detection limit for the river silt, however,
 was 1 ppm.
       Table 5  gives the analysis of the total carbon of the soil in % carbon. It was
 important, that the indigenous carbon content of the soil be low, in order to guarantee
 that the toxicant-carbon be used as prefered source of carbon and energy by the soil
 microbial communities.
B. Screening  tests
        The optimal loading rates for the soil/waste mixtures were determined by
 evaluation of both indigenous microbial population performance and acute toxicity
 leachate analysis. Microbial ATP analysis showed initial stress on the indigenous
 microbial populations through day ten for both waste types (Fig. 4). The microbial
 populations had recovered by day 17. Lagoon wastes, applied at 4% and 8%, showed
 minimal stress, and microbial ATP levels increased by day  17.  Buried wastes showed
 comparable results for 2-4% waste loadings at day 17. Microbial diversity indices agreed
with ATP predictions, which suggested maximum loading rates of 8%, for lagoon
 wastes, and 2-4% for buried wastes (Fig. 5).  EC$Q levels of leachates (Fig 6) predicted
4.0-9.5% waste weight (wet basis) for lagoon waste  and 2.5-6.0% waste weight (wet
basis) for the buried waste.Two soil mixtures, soil type I, comprising of one part river
                                     17

-------
TABLE 3:  Primary F-2 Constituents in CERCLA waste site
                                    Buried      Lagoon     River
     Compound                     Wastes     Wastes       Silt
(control)
1. naphthalene
2. 1-methylnaphthalene
3. 2,3-dimethylnaphthalene
4. 2,3,5-trimethylnaphthalene
5. biphenyl
6. fluorene
7. dibenzothiophine
8. phenanthrene
9. anthracene
10. 1-methylphenanthrene
11. fluoranthrene
12. pyrene
13. benzo(a) anthracene
14. chrysene
15. benzo(b) fluoranthrene
16. benzo(e) pyrene
17. benzo(a) pyrene
18. perylene
19. acenapthene
20. substituted benzene
21. indenopyrene
22. benzo(g,h,i) perlene -^
23. dibenzo(a,h) anthracene
24. benzo(k) pyrene
25. unknown (E.T. = 24.51)
920
1200*
600*
600*
1700*
850
800*
4528
2257
800*
930
830
470
340
ND
ND
<100a

2300
1500*
1100
1200
100 +
ND
ND
1500
1700*
1000*
700*
1600*
1300
1200*
5757.
3227
600*
1686
1059
570
550
ND
60
215

1466
2100*
321
1432
357
457








trace
trace


trace
trace
trace
trace










-------
TABLE 4:  Primary F-l Constituents in CERCLA waste site
Compound
C-12
C-13
C-14
C-15
C-16
C-17
PRISTANE
C-18
PHYTANE
C-19
C-20
C-21
C-22
C-23
C-24
C-25
C-26
C-27
C-28
C-29
C-30
C-31
Buried
Wastes
800 ppm
1100 ppm
1100 ppm
1400 ppm
1700 ppm
1400 ppm
4300 ppm
1200 ppm
2700 ppm
750 ppm
370 ppm
240 ppm









	
Lagoon
Wastes
2300 ppm
3600 ppm
4000 ppm
4300 ppm
5000 ppm
4400 ppm
3100 ppm
4900 ppm
1500 ppm
3300 ppm
2900 ppm
2000 ppm
1400 ppm
840 ppm
600 ppm
890 ppm
300 ppm
320 ppm
*
1200 ppm
560 ppm
330 ppm
       Below detection limit of 100 ppm
       Lab contaminant
                                19

-------
            TABLE 5:  TOC analysis (% carbon) of (he 4% lagoon and 4% buried waste mesocosms over 35 day
                      (ime frame
            TIME
4% LAGOON TL41
4%  BURIED (B41
CONTROL SOIL
NJ
O
pure waste
dayO
day?
day 14
day 21
day 28
day 35
day 42
(after
reapplication)
22.847
6.256
5.620
5.625
8.110
9.228
' 4.753
12.197
-
5.295 0.771
4.777
4.152
4.831
5.080
4.816
8.395

-------
   Fig. 4:     Microbial ATP profile of screening  test mesocosms
                	DAY1
  0% SOIL TYPE 1
    12% BURIED
  "j  8% BURIED
  55  4% BURIED
  O  2% BURIED
  212% LAGOON
  ^ 8% LAGOON
    4% LAGOON
    2% LAGOON
              0  10 20 30 40 50 60 70 80 90 100110120
            RELATIVE LIGHT UNITS ( x 100/g dry wt soil)
                                         DAY 10
0% SOIL TYPE 1
  12% BURIED
 "j 8% BURIED
 m 4% BURIED
 DC
 « 2% BURIED
 c£
 dl2% LAGOON
 ^ 8% LAGOON
  4% LAGOON
  2% LAGOON
                        33
             0  10 20 30 40 50 60 70 80 90100110120
           RELATIVE LIGHT UNITS (x 100 /g dry wt soil)
                                         DAY 17
0%SOILTYPE1
   12% BURIED
$ 8% BURIED
2 4% BURIED
^ 2% BURIED
=i 12% LAGOON
5? 8% LAGOON
   4% LAGOON
   2% LAGOON
            0    20    40    60    80    100   120
           RELATIVE LIGHT UNITS (x 100/g dry wt soil)
                                    21

-------
   Fig. 5:     Microbial diversity  of screening test mesocosms
01
OT
<
til
oc
o
08
     SOIL7YPE1
     12% BURIED
8% BURIED
      4% BURIED
      2% BURIED
                    100    200    300    400    500

                         COLONY FORMING UNFTS
                                                600
                                                      • BAC
                                                      0 PGI
01
Ol
oc
     SOILTYPE1
    12% LAGOON
     8% LAGOON
     4%LAGOON
     2%LAGCON
                     100    200    300   400    500    600

                          COLONY FORMING UNITS
                                                          BAC
                                                          R3
                  BAC: Bacteria & actinomycetes (x 10 /g dry wt)
                  FGI: Fungi & yeasts (x 10 /g dry wt)
                                      22

-------
Fig. 6:     Microtox™ analysis of WSF  of screening test mesocosm
     SOILTYPE1
 w  12% LAGOON

 O)
 <
 UJ

 §   9%LAGOON

 08
     6%LAGOON
    3% LAGOON
              0     20    40    60    80    100  120   140
    SOILTYPE2
uj   12% BURIED
03
<
LU

O    9% BURIED
•a
3?    6% BURIED
     3% BURIED
                               51.3
                         232.
              0    20    40    60    80   100   120   140

                                EC 50
                                23

-------
silt and two parts sandy clay (1:2), and soil type II, comprised of two parts river silt and
one part sandy clay (2:1) were tested. Soil type n, when mixed with the waste, was
found to be unsuited for subsequent investigations, because the indigenous microflora
exhibited stress when exposed to that mixture. Soil type I, when mixed with the waste
did not impose stress and was therefore selected for subsequent task n and task m
studies. Loading rates of 4% for lagoon waste and 2.5% for the buried waste were,
therefore, shown as mean optimum loading rates. These rates  of waste application were
therefore used in  task II mesocosm studies. A 4% O&G loading rate of lagoon waste
required 799.9 g of pure waste sludges to be incorporated into soil mixture I (1.5 kg
river silt mixed with 3 kg sandy clay). Soil waste preparations for 4% O&G buried
waste mesocosms required 274.0 g of pure waste sludges, for 2.5% O&G buried waste
167.4 g and for 2.5% O&G lagoon waste 468.7 g. All these quantities were determined
for 4.5 kg of soil mixture L
C. Mesocosm Tests
2. Chromatographic Analysis
       Fig. 7 shows GC/FID chromatograms of the F-2 fractions for lagoon waste
mesocosms with a 4% loading rate and the control soil All compounds show a decrease
over the 35 day incubation period The total gross removal over this time period is
almost 70%. Fig.  8 presents GC/FID chromatograms  for the aliphatic fraction (F-l)  for
4% lagoon mesocosms over the same time period. The total  removal is 90.85%. The
same degradation pattern, but at a faster rate is observed for the 2.5% Lagoon waste
loading (Fig. 9). The total removal for the 2.5% loading F-2 fraction is 96.95% (Table
6).
                                     24

-------
                1C/1 __ ai	ysis
                day time frame
-„-• 4'.. .agt_..
       d-0
,, F- . _rat._..i o._- a
     Phenanthrene
       Anthracene
                                                                         •.AW..lW*.	_,v.
       d-7
             _•	t_

                                                        -Av+AJPlAv^iMj
       d!4
       d-28
to
                                    L/uw^JLMukAJt^A^A^
      d-35
      Control
      Time 5.0 mln
                                Time 26 mln

-------
      Fig. 8:    GC/FID analysis for 4%  lagoon waste, F-l fraction  over a 35


                day time frame



                                  C-15
       d-0
            n.A         C-16  c.1?       C-19
            1-14               v"*'/^ifl      /~« if\

     C-13     I                   -    C'18  I    C-20





jL^^JLAA^ojJwAviXJ^^^
       d-7
             ^A^^—.». K ^. r -vA Jvi^ AA<_)l^*-ir-tA*N-^-. ,M^ ^Jl—, ^A> IM ^^ A>. ^ l|_
       d-14
                    ju^JU/vJJWvi^^
to
                                       -J	1.
                                                              '   *    '
      d-28
                    .^^^JLrAMU^/U^
                                               -1	1	lu
       d-35
          *
                                                              '*''**•    *   *
       Control
       Time 5.0 min
                                                                                         Time 34.25 m

-------
         Fig. 9:    GC/FID analysis for 2.5%  lagoo   /aste, F-2 fraction  over a
            d-0
                    35 day time frame        Unk. 15.3  Phenanthrene
                                                            II Anthracene

            d-14
to
            d-21
            d-35
                                                      •     '	'     '
                          ^^jj^^^
                                                      ^
                                                                     1 - 1 - 1 - 1 - 1 - 1 - 1 - ' - "
                                ,Ju^_«A*>rV--K*_A^~A>Ai— J LJ^v
                            >*k4*«r*_/V*
«..K X.




I	I	I	I	1	1	1	1	1	1-
                                                        .•.> .Av
            Time 7.0 min                                                                          Time 28.0 min

-------
TABLE 6:  F-2 fraction;  2.5% lagoon waste.


Compound               0
Total (Gross)
            Time in days
             7          14
1900
860
710
                         21
340
                        35
NAPHTALENE
METHYLNAPHT.
DIMETHYLNAP.
UNK (Rt-11.1)
UNK (Rt=11.4)
TRIMETHYLNAP.
ACENAPHTALENE
UNK (Rt=12.7)
UNK (Rt=13.2)
BIPHENYL
FLUORENE
UNK (Rt=15.3)
UNK (Rt=15.8)
UNK (Rt=15.9)
UNK (Rt=16.2)
DIBENZOTfflO.
PHENANTHRENE
ANTHRACENE
UNK (Rt=18.4)
UNK (Rt=18.9)
UNK (Rt=19.0)
1-METHYLPHEN.
UNK (Rt=19.9)
UNK (Rt=20.8)
FLUORANTHENE
PYRENE
18.0
19.0
13.0
28.0
25.0
7.0
20.0
82.0
48.0
19.0
30.0
170.0
50.0
21.0
24.0
21.0
138.0
97.0
33.0
27.0
33.0
17.0
51.0
28.0
37.0
1.0
>0.1
>0.1
>0.1
16.0
14.0
0.6
11.0
64.0
39.0
12.0
13.0
120.0
32.0
>0.1
15.0
8.0
68.0
42.0
36.0
16.0
18.0
11.0
33.0
17.0
22.0
11.0
>0.1
>0.1
>0.1
>0.1
1.0
>0.1
3.0
42.0
20.0
5.0
3.8
119.0
36.0
>0.1
12.0
7.0
42.0
38.0
44.0
13.0
18.0
12.5
39.0
22.0
32.0
20.0
>0.1
>0.1
>0.1
>0.1
>0.1
>0.1
1.6
39.0
15.0
1.2
>0.1
75.0
25.0
>0.1
>0.1
>0.1
7.8
22.0
22.0
2.9
5.2
4.4
9.5
9.0
14.0
11.0
>0.
>0.!
>0.!
>Q..
>0.
o.:
u
o.:
>0.1
O.f
13.C
>0.1
>0.1
>0.1
>0.1
1.3
4.2
3.3
0.3
>0.1
0.7
>0. 1
>0 1
4.0
13.0
58
NOTE—The above numbers are accurate to only 2 significant fig.
      concentr. in ppm
                                   28

-------
2. MicrobialATP
       Microbial ATP contents of the soil/waste mesocosms were monitored over the
course of the experiment. Over the first 35 days, the microbial activities of all
experimental mesocosms in the 4% L-series were higher than those of the control
mesocosms (Fig. 10). Gradual biodegradation was evident during the first 14 days. The
highest microbial activity was evident on day 21 for the lagoon mesocosms, which
correlates with the kinetics of toxicant disappearance. At this point, the indigenous
microflora have adapted to the waste.
       Fig. 11 shows the  ATP activity for 1L2 - 3B2 as compared to the controls over
the first 35 days of the experiment The microbial activity was initially high in all
experimental units, with a characteristic suppression of growth on day 14. The low
activity in the control mesocosms after day 2 was probably a result of nutrient depletion,
while the subsequent increases in activity at days 28 and 35 resulted from the application
of another nutrient amendment at day 27. Partial reason for the decrease in activity at day
14was moderate desiccation  of the mesocosms. The moisture at this day had dropped
to less than 9% for the buried mesocosms. It has been reported in the literature, that
microbial activity becomes marginal once moisture drops to less than 10% water holding
capacity (9). Rewetting of the mesocosms with sterile deionized water increased the
moisture level. Good recovery, indicating the re-adjustment of the microorganisms to the
waste, was evident at day 21.
3. Microbial diversity
       Microbial diversity estimates for laboratory mesocosms, as calculated by
determining the mean number of CFU on triplicate plates is presented in Fig. 12. At day
21, the experimental mesocosms exhibited significantly more growth than the control
mesocosms. Counts were high for all mesocosms throughout the experiment indicating
a microbial consortium present at all times. Further monitoring of microbial diversity
through day 84 is presented in Fig.  13. The fact, that such a high density as well as
                                        29

-------
RELATIVE LIGHT UNITS /g dry wt soil
           111
                                                  RELATIVE LIGHT UNfTS /g dry wt soil
                                                                                      Tl
                                                                                      (5*
11
-•• »N|
.*>.
                                                                                      o
                                                                                      3
                                                                                      CT
                                                                                      5T
                                                                                      TJ
                                                                                      •D

                                                                                      o
                                                                                      «*»

                                                                                      to
                                                                                      (Q
                                                                                      o
                                                                                      o
                                                                                      3

                                                                                      3
                                                                                      
-------
  RELATIVE UGHT UNITS /g dry wt soil
                                 RELATIVE LIGHT UNITS /g dry wt soil
o
 OJ
o
 en
o
 at

                                                                       111
                                                                       -«. -4 10
o
o
cr

si!

>

TJ

-i
o
—Jj

CD"

o^

10
                                                                          (O
                                                                          o
                                                                          o
                                                                          3


                                                                          O
                                                                          (0
                                                                          o
                                                                          o
                                                                          o
                                                                          0)
                                                                          3
                                                                          (0

                                                                          "E
                                                                          c
                                                                          to

                                                                          O
                                                                                                  Q.
                                                                                                  0)
                                                                                                  ro

-------
Fig. 12: Microbial diversity of mesocosms plus C1, day 7-35
8
C3
|
X
§
•a
§
CO
            CS1  CS2  CS3   1L4   2L4   3L4   C1

                         MESOCOSMS
  I
  O
  •0
  d
           CS1   CS2   CSS   1L4   2L4  3L4   C1
                         MESOCOSMS
                                                     DAY?
                                                     DAY 14
                                                    DAY 21
                                                    DAY 28
                                                    DAY 35
                        32

-------
   Fig. 13: Microbia! diversity of mesocosms, day 28-84
 O
 W
 GQ
1
O


O)
     50
 ~   40 H



 £
 2   30H
         CS1  CS2  CS3  1L4  2L4  3L4  1B4 2B4  3B4


                       MESOCOSM
      50-i
40-
30-
                                                     D84
                                                     D77
          CS1 CS2 CSS 1L4 2L4 3L4 1B4 2B4 3B4


                       MESOCOSM
                        33

-------
diversity of all four general groups of microorganisms was maintained, indicated that
the requirements of a genetic pool to emphasize biodegradative kinetics were evident
Microbial diversity of fungi and yeasts were generally comparable to the bacterial
counts. Fungal counts were high throughout the whole course of the experiment, while
the yeasts were always present in low numbers. This relationship is characteristic of
many agricultural soils (51). The SPC-plate counts for fungi and yeasts indicated a well
adapted fungal population with highest overall counts in the 4% and 2% lagoon
mesocosms. These mesocosms seemed to provide an extraordinarily good habitat for the
fungi. Generally, SPC data reflects only variations in colony forming units and does
not provide interpretations on either total biomass or actively viable biomass. It was
intended to only reflect  the transient microbial morphology of control and amended
soils during the experimental time frame indicated so as to insure that a genetic pool
capable of toxicant biotransformation was present at all times. At no time during the
experiment was there evidence of acute or chronic toxicity to these adapted microbial
populations.
4. Microtox™
       Microtox results were provided by EPA laboratories in Ada, OK. Microtox™
toxicty results for the WSF fractions of the mesocosms over a 35 day time frame are
presented in Fig. 14. Data, shown as LL5 value, is defined as the relative mean % loss
of bioluminescence in the WSF samples over a five minute time period. The 20% value
marks the threshold between the non toxic state  (LL5 < 20%)  and the toxic state (T.T.5 >
20%). If LL5 values were found to be less than -5%, this was reported as stimulation of
bioluminescence. Stimulation was observed for the control mesocosms (CS) from day
zero to day 35. For all the other experimental mesocosms of the 4% - and 2.5% loading
rates, TJ-S values were constantly  high indicating a highly toxic response by the test
bacteria. Technician error may have contributed to inaccuracies in the analysis of this
data set Additional sources of error may have been introduced by various interferences
                                       34

-------
 LL5 (Relative
 Mean % Loss of
Blolumlnescence
In WSF Samples)   20..
                         Fig. 14:     Microtox Toxicity
                             Results of Mesocosms
 ics

 1L4

 I L2

EJB4

DB2
                                       Day
                                         35

-------
with the Microtox™ test system. The test organism, being a marine microbe,  may have
been repressed by intermediates formed in the soil or partially solubilized metals present
in the river silt Plating studies and microbial activity estimations of the adapted,
indigenous microflora did not exhibit repression over this time period. For these
reasons, Microtox™ is still being evaluated for assay of oil-contaminated terrestrial
ecosystems (Matthews, personnel communication). Despite the Micotox™ results, EPA
approval was obtained for the field verification studies; decisions were based on GC/MS
and microbial activity data.
5. Commercial inoculum test
       Fig. 15 shows a series of Total Ion Chromatograms (TIC) of the mesocosm
inoculated with the commercial inoculum (Cl). This mesocosm contained 4% lagoon
waste plus commercial inoculum.The upper TIC chromatogram  presents  analysis for
day zero while the middle TIC chromatogram presents analysis for day 35. Separation
into aliphatic and aromatic hydrocarbons was not possible when preparing these samples
for GC analysis. High biomass interferences were noted.When comparing the upper
two chromatograms to the PAH standard (lowest TIC), it was evident, that all of the
compounds analyzed exhibited a decrease in concentration over time. The appearence of
unresolved compounds as expressed in the high baseline for day 35 was probably due to
complex organic molecules produced by the microorganisms. This was consistent with
our difficulties in F-l/F-2 fractionation as mentioned and further documented by HPLC
(not represented here).
       The degradation rates for acenapthene, anthracene and phenanthrene by the
indigenous (adapted) and commercial inoculum are shown in Fig. 16 and Fig. 17. The
commercial inoculum showed an  enhanced degradation rate for these compounds over
the first 14 days of the experiment The rate of degradation was almost linear for this
time period observed. The degradation pattern for the 2.5% lagoon waste  began at a
similiar kinetic rate and slowed slightly for anthracene and phenanthrene. Acenapthene
                                         36

-------
Fig. 15:     Total ion  chromatograms of mesocosms with commercial


            inoculum
 TREATMENT OftY-0
 nc a
 B. BC4-]
 B.BC4




 7. BC4




 i.BC4




 3.BC4




 4.BC4




 3.BC4
     f V3iCL?.Q
 1BQQQ



    a
TREATMENT OAY-35
 TIC of V3.CL43.D
 SBBB
 4BBB
 aaaa
 aaaa
 1QOB
PftH STANDARD  25 NG
TIf .
l.BEd
1.4C4-
t.SC4;
laaaa;
BQQO;
SDpQ;
SBBQ:
' va'ST«'






*^. •
° VP
Q











1







9Q















1
.
SB dQ
                                                 37

-------
Fig. 16:     Degradation rates for commercial inoculum as compared

            4% adapted inoculum
         100
                                  Commercial Inoculum
                                                        •a-
                                                        -»- ANTHRACENE
                                                        •» PHBMANTHFBvE
                      10        20

                         Time (days)
 30        40

  4% LAGOON
        100
                                    Adapted Inoculum
                     10        20

                    TIME (days)
                                                        •o ACBVJAPTHALENE
                                                        -^ PHBJANTHRBE
                                                        -B- ANTHRACENE
30        40

 4% LAGOON
                               38

-------
Fig. 17:     Degradation rates for commercial inoculum as compared to

            2.5% adapted inoculum
              100-
                                      Commercial Inoculum
                          10        20

                             Time (days)
                                                            -a- ACBMAFTHEM
                                                            -»- ANTHRACENE
                                                            «• PHENANTURB
                               30        40


                                4% LAGOON
        a
        Z
        z
        LU
        CC
        LU
        QC
        (ft
              100-
80-J
60 i
               40-
20-
                                                                ACENAPHTENE
                                                                ANTHRACENE
                                                                PHENANTHFe
                     0       7      14      21      35


                          TIME (DAYS)          2.5% LAGOON
                                 39

-------
was degraded at a linear rate (Fig. 17). This biodegradation response, at 2.5% O&G,
would be expected since the waste concentration was lower and consequently less toxic
than the 4% loadings. The adapted mesocosms at the 4% load show an initial lag task.
No degradation was observed over the first 14 days of the experiment. Following day
14, however, there was a significant increase in toxicant degradation rates.  Both rates,
at concentrations at or approaching 20% of the original toxicant toxicant addition for both
adapted and commercial inoculum, leveled out over time. Final residual concentrations
for both inocula were similiar. At the conclusions of these investigations, no noticeable
variation in biotransformation/ degradation by the commercially available mutated
bacterial cultures over the adapted cultures was evident
6. Sequential Re-loading of 4% and 25% Mesocosms
After substantial waste reduction had been evident in the first 35 days of the experiment,
the 4% and 2.5%  mesocosm series were reloaded with identical waste concentrations.
Soon after waste incorporation, it became evident, that the 4% lagoon mesocosms were
overloaded as indicated  by decrease in microbial activity (Fig. 13). The soil/waste
mixture was too heavy and dough-like. More soil was therefore incorporated into the
mixture to compensate and render it more workable. A 4% reloading rate was deemed
too high for subsequent applications. It was observed from data generated in the 2.5%
reloading experiments, that 2.5% would be an acceptable rate for waste re-applications in
the given time frame. Subsequent monitoring of microbial ATP and microbial diversity
reconfirmed no induced stress of the microbial communities under these waste
concentrations.
7.HPLC
       High Performance Liquid Chromatography (HPLC) analyses were performed
weekly to monitor general trends in toxicant concentrations. Fluorescence detection
using an excitation wavelength of 254 nm, and emission wavelength of 375 nm, was
used in all cases. Peak identification was by comparison with PAH standards. Several
                                     40

-------
major problems rendered interpretations of data sets difficult. The major problem
consisted of poor reproducability of replicate samples due to mechanical error on
autosampling coupled with sample variability. Thus, for example, significant sampling
differences were noted for the 2.5% O&G buried waste, while other waste treatments
showed similarities between the individual replicate mesocosms. Achieving the
necessary homogenicity in test samples was not possible due to unfavorable mixing
properties of the oily sludges with the soils. Also observed was an increase in baseline
of the respective chromatogram caused by accumulation of unresolved polar organic
compounds on the column. This phenomena was also observed by GC/MS and will be
discussed in section "discussion". Another problem associated with the use of
fluorescence detection was that anthracene, one of the key compounds, saturated the
detector at concentrations more suitable for the detection of other PNA's. This was due
to its high fluorescence response compared to other PNA's. Generally, HPLC results
for 4% and 2.5% loading rates illustrated similiar trends in waste reduction up to day
35. After reloading, however, no continued reduction was evident indicating that the 4%
sequential load was excessive. For these reasons, this data was not presented in the
context of this report However, all HPLC samples were retained and will be re-analyzed
at such time when equipment limitations have been corrected. Appendix C shows HPLC
chromatograms for the buried waste (1B4,2B4,3B4). These are presented to indicate
trends only.
 8. Soil Moisture andpH
These parameters were monitored consequently over the whole course of the
experiment. No significant changes in pH were observed, while moisture fluctuations
were controlled by periodic addition of sterile water. Again, it was noted, that %
moisture below correlated with ATP responses. Higher microbial activity was always
observed when moisture contents were at maxima. The pH, however, did not seem to
have an effect The results are presented in Table 7 andTable. 8.
                                     41

-------
           TABLE 7: % Moisture of Laboratory Mesocosms
                                                 Time in days
           Mesocosm       25           7           10         14         21         28        35
N>
CS1
CS2
CS3
1L4
2L4
3L4
1B4
2B4
3B4
1L2
2L2
3L2
1B2
2B2
3B2
B20
B21
L20
L21
Cl
C2
3.00
5.50
3.85
22.78
19.56
19.80
7.50
5.28
6.52
9.21
8.19
9.01
6.10
8.29
6.34
1.49
8.05
4.15
2.18
1.41
3.24
6.68
10.62
18.54
26.45
22.14
24.57
10.00
8.86
8.30
17.22
15.68
16.52
11.36
11.93
14.32
8.05
11.73
8.39
7.01
8.52
8.54
7.01
12.10
9.66
29.47
23.27
24.98
8.57
7.91
8.39
9.86
14.83
17.86
10.41
11.71
13.42
13.67
15.49
27.08
25.01
24.11
12.56
7.78
11.24
9.85
29.45
24.42
26.25
10.86
9.59
8.92
20.95
14.81
18.75
13.05
12.08
14.04
13.75
17.28
28.62
24.36
27.33
13.12
3.78
7.09
6.18
20.60
16.54
22.16
6.79
6.69
7.07
11.52
10.63
15.09
8.99
8.67
13.05
8.10
11.53
21.33
19.76
13.88
10.31
6.63
12.28
10.04
24.86
20.37
23.42
8.22
8.37
8.77
16.10
13.77
17.53
11.31
11.14
17.41
23.21
14.11
27.70
24.96
15.91
13.65
8.84
12.58
9.63
27.69
22.61
22.83
8.26
9.00
9.04
15.63
13.83
18.40
11.48
13.78
13.90
12.91
14.83
23.88
30.11
23.54
12.59
8.76
10.71
9.24
23.81
15.83
18.54
7.26
7.61
7.76
13.88
11.75
13.72
10.18
13.72
11.55
9.81
13.73
28.23
38.34
17.18
12.02

-------
TABLE 7 (cont'd):
Mesocosm
% Moisture of Laboratory Mesocosms
                    Time in  days
   42               49
77
84
CS1
CS2
CS3
1L4
2L4
3L4
1B4
2B4
3B4
1L2
2L2
3L2
1B2
2B2
3B2
B20
B21
L20
L21
Cl
C2
9.45
13.59
11.83
37.75
37.75
35.30
12.48
7.71
6.37
14.12
15.96
18.82
11.49
12.77
12.42
10.86
12.23
24.10
19.91
21.41
12.18
10.47
37.66*
37.34
38.21
13.13
12.23
10.80
-
-
_
.
-
5.99
6.69
8.27
34.41
33.72
45.21
10.99
10.92
9.01
-
-
-
-
-
5.81
6.67
9.25
29.88
32.44
31.33
7.45
10.01
8.57
-
-
.
_
-
      * After waste re-application for 1L4 - 3B4.

-------
ol
              TABLE 8:  pH Profiles of Laboratory Mesocosms

                                                             Time in days

              Mesocosm        257           10          14         21           28         35
CS1
CS2
CS3
1L4
2L4
3L5
1B4
2B4
3B4
1L2
2L2
3L2
1B2
2B2
3B2
B20
B21
L20
L21
Cl
C2
8.25
7.82 '
8.17
7.28
7.21
7.23
7.90
7.91
7.96
7.34
7.38
7.45
7.55
7.58
7.54
3.42
8.14
8.55
8.44
8.4
8.5
6.05
6.87
6.87
6.93
6.95
7.11
6.05
6.64
7.52
5.86
5.79
6.99
6.94
6.97
5.88
5.65
6.89
7.17
7.16
6.99
6.87
7.23
7.25
8.34
6.78
6.28
5.94
6.11
6.85
6.67
6.52
6.50
6.50
6.40
6.43
6.40
6.95
6.90
6.23
6.63
6.13
7.16*
8.55
7.56
8.12
6.39
6.51
6.53
6.82
6.81
6.75
6.75
6.81
6.56
6.57
6.56
6.54
6.53
6.54
6.42
6.51
6.48
6.72
8.40
7.89
7.86
6.61
6.58
6.55
6.97
7.07
7.01
6.76
6.78
6.81
6.77
6.80
6.67
6.58
6.59
6.69
6.58
6.58
7.08
8.05
7.52
7.98
6.70
6.63
6.93
7.06
7.02
7.01
6.79
6.80
6.91
6.87
6.80
6.88
6.91
6.64
6.72
6.98
6.90
7.30
8.38
7.59
7.74
7.41
7.06
7.19
7.21
7.07
6.98
7.30
7.40
7.36
7.26
7.13
6.97
6.74
7.13
7.15
7.03
7.26
7.42
8.22
7.77
8.25
7.73
6.83
6.93
7.61
7.91
7.66
8.34
7.37
8.00
7.36
7.33
6.94
7.27
7.02
6.91
6.89
7.13
7.71
                   * Waste application at day 6.
                   * pH measurements: Ig soil in 99ml distilled deionized H20

-------
ui
           TABLE 8 (cont'd):  pH  Profiles of Laboratory Mesocosms
                                                         Time in days
           Mesocosm              42               49               77               84
CS1
CS2
CS3
1L4
2L4
3L4
1B4
2B4
3B4
1L2
2L3
3L2
1B2
2B2
3B2
B20
B21
L20
L21
Cl
C2
7.98
7.63
7.94
6.95
6.90
6.79
6.77
6.96
6.74
7.42
7.44
7.53
7.45
7.35
6.89
6.48
6.62
6.58
6.85
6.72
6.97
8.31
-
-
* 7.02
7.26
7.20
7.06
7.08
6.91
_
-
-
_
-
-
_
-
_
-
.
™
8.12
7.38
7.85
6.97
6.88
6.92
6.28
6.10
6.08
»
-
-
_
-
-
_
-
_
-
_-
~
7.87
7.52
7.75
7.24
7.06
6.97
6.50
6.31
6.26
_
_
-
^
_
-
_
-
_
-
_
••
                 * After waste re-application for 1L4 - 3B4

-------
D. Field Verification Studies
       1. Chromatographic Analysis
       Field studies, coordinated under IT personnel, were conducted at the Old Inger
site. Data from pilot verification studies is shown in Fig. 18. These GC/FID chromatograms
represent analysis of the first waste application for both group I and group n plots over a
25 day time frame. The first chromatograms of each series were from the control plot
before waste application. Complete differentiation into the two fractions, F-l and F-2, was
not possible, because the waste was different in hydrocarbon composition than the waste
used in the previous task n mesocosm studies. Additionally, the waste contained a lesser
amount of PAHs than the one tested in the laboratory. A composite of the F-l and F-2
fractions therefore was used for analyses. However, this did not pose a problem from
analytical detection capabilities involved, but supported GC/MS data of original wastes
being predominantly F-l. The general trend of hydrocarbon reduction as observed in task
n studies was evident It was important to note, that the residual components, found at day
25, were determined by GC/MS to be primarily F-l  straight chain hydrocarbons,
respectively C-17 and C-19 aliphatics.
       Subsequent re-analysis of F-2 fractions at the end of the experiment were
conducted for group I and n where group I analysis is presented in Fig.  19. On day 65,
only a small residue was left as a consequence of the first waste application. Reloading
group I with 2.5% buried waste was completed on day 102. This additional waste
application at day 102 was responsible for the increase in  hydrocarbon content by day 127
as compared to day 65.  By  day 156, a further increase in F-2 content along with substantial
biotransformation as expressed by increase in baseline was obvious. This unexpected
increase could probably be attributed to sample variability and poor mixing of the soil/
waste components in the field plots.
       Subsequent group n re-analyses of  F-2 fractions are presented in Fig. 20. On day
46, first reloading of these plots with 2% lagoon sludge was applied.  Samples analyzed
                                         46

-------
                         IS
  group I
  before waste application
Fig. 18:    GC/FID analysis for group I and  group II over a 25  day
            time frame
  d-l
  d-25
               ^>	^JU>
Time 7.0 min      IS=Intemal Standard
group
before waste application
                                                      Time 19.0 min
d-25

-------
        FIG.  19:    GC/FID analysis for group I, F-2 fractions, day  65  -156




         day  65
oo
         Time: 10.0 min
         day 127
         Time: 10.0 min,
                              _J	•   >
                PNA STD
                                            '   '   [   '  f.
        Time: 10.0 min
                                                                                               Time: 41.0 min
Time: 41.0 min
                                                                                                          .11
                                                                                            •    '   •
 Time: 41.0 min

-------
FIG. 20:   GC/FID analysis for group II, F-2 fractions, day 46-156


day 46
                              il i  Al     , A A  ,  J    I

                                                    -vUwuAKK_K^_
Time: 10.0 min
day  65
                                                                                    Time: 41.0 min
Time: 10.0 min
day 79
                                                                                    Time:  41.0 min
Time: 10.0 min
Time: 10.0 min
Time: 41.0 min
Time: 41.0 min

-------
on day 65 showed a consequent reduction of waste. The noticeable increase by day 79
without additional waste application is probably due to sample variability, or collection
from an alternate part of the fieldplot. Sample variability was one of the major problems of
the experiment and will be discussed in Section V, Discussion. The chromatogram of day
102 reflects the third waste application for these plots. This was the first time the plots
received a load of buried waste (2.5% O&G). The mixing of buried with lagoon waste in
the same plot rendered tracing the fate of one or more individual key toxicant significantly
more complicated. GC chromatogram interpretations were impeded, because general
disappearance patterns of the toxicants were no longer apparent Hydrocarbon
accumulation was observed from day 127 over day 156 to the end of the investigations.
This indicated overloading of these plots as had also been confirmed by Microtox™ (and
plant biomass determinations, see section IV, D 6). The fourth waste applicatiori with 2.5%
buried waste on day 156, was therefore excessive.
       To conclude,  hydrocarbon biotransformation/degradation fates were re-evaluated
after a time period of human inactivcness. Samples were taken four months after the last
activity at the field had been carried out, because there was a concern about overloading of
the group n experimental plots. At this time, plants were established in the field plots.
Samples were analyzed in two increments to a depth of ten to twelve inches to study the
residue levels and downward migration of organics. In addition, four surface samples (0"-
6") were taken from alternate locations of PL5 to evaluate uniformity in samples, and better
explain previous variation in analytical results due to sample and hydrocarbon content
variability. No fractionation into F-l and F-2 fractions was done for these last samples,
because the wastes were already too heavily weathered and metabolized
       Plot four exhibited limited toxicant downward migration to a depth of 12 inches
as was confirmed by TOC data (see Fig. 31). No  migration was evident for the other
plots up to the tested depth of ten to twelve inches. Fig. 21 depicts a depth profile for
PL5. The large area of unresolved compounds indicates, that substantial
                                          50

-------
FIG. i*.    GC/FJD analysis for PL5, 0"-20"
             0"-6"
                                                                              sv
            Time: 3.0 mi
mm
Time:  38.0 min
Ul
                          IS
                                   j  I   LsX
                                   ul^
            Time: 3.0 min
                                                              phthalate
                                                                          Time:  38.0 min
              16"-20"
                          IS
                                        phthalate
            Time: 3.0 min
                                                                         Time:  38.0 min

-------
biotransformation took place in the upper six inches of the soil with only a few
identifiable compounds left. It was of great interest to note therefore, that even with
application of large quanities of toxicants, no significant migration took place into the
deeper soil layers.
       Fig. 22 depicts four chromatograms of PL5 from different locations as shown
below.
                           PLS
       It can be seen (Fig. 22), that there was no homogenicity in the samples indicating
poor mixing of the waste with the soils in the field. The tendency to clump and oily
contents of the soil rendered mixing by field engineers difficult Better methods for
future soil/waste incorporations need therefore to be applied to promote uniformity in
samples. Such better methods could also favor microbial biotransformation while
providing a better microbial habitat
2. Microbial ATP
       Microbial ATP estimations for the field plots over the total time period of 184
days are shown in Fig. 23 - 25. Day zero is actually the second day after the first waste
application. The microbial activity was  depressed through day ten, but recovered  well,
by day 23.  This recovery was favored by the application of fertilizer, (application rate 5
Ib /plot 10-5-5) and tillage for better oxygenation at day 14 for all five plots. All
fieldplots at day 23 showed higher activity than the control plot (Pll). The group I
experimental plots (P12.P13) (Loaded with 4% buried waste) show less microbial activity
than the group n (P14,P15) Goaded with 4% lagoon waste) experimental plots. There
was also activity suppression observed at day 30. This probably resulted from
reloading of the group n plots with another 2% loading rate. In contrast, group I and the
                                             52

-------
FIG. 22:    GC/FID analysis for alternate  locations of PL5, 0"-6"
         Time: 3.0 min
             D
                                                                                               Time:   46.0 min
                                                                                                Time:  46.0 min
Time:   46.0 min
         Time: 3.0 min
Time:   46.0 min

-------
RELATIVE LIGHT UNITS /g dry wt soil
            111
RELATIVE LIGHT UNITS /g dry wt soil
                                                                                             •n
                                                                     o
                                                                      to
             ooo
                                                                   -* o> o
                                                                   o
                                       to
                                       O)
                                                                                             o
                                                                                             ~t
                                                                                             o
                                                                                             g;
                                                                                             o^

                                                                                             5"

                                                                                             o
                                                                                             O
                                                                                             »-••
                                                                                             W
                                                                                             ««

                                                                                             a
                                                                                             0)
                                                                                             •<
                                                                                             o
                                                                                              I
                                                                                             CO

-------
  Fig. 24: Microbial ATP profiles of field plots, day 51-113
o
(A
CD
UJ
Ul
DC
                    PL2     PL3      PL4

                         FIELDPLOT
                                           PL5
                                                        DAY 51
                                                        DAY 58
                                                        DAY 65
b
D
I-
o

      10
                    PL2      PL3     PL4

                         RELDPLOT
                                                       DAY 102
                                                       DAY 106
                                                       DAY 113
                                 55

-------
RELATIVE LIGHT UNITS /g dry wt. soil
                                             RELATIVE LIGHT UNITS /g dry wt soil
                      •n
                      (5*
                                      3
                                      m
                                      §
            11
II
^i en
o o>
                                                                                Ol
                                                                                O)
                                                                                _A
                                                                                09

-------
control plot at this day showed extreme suppression. The cause for this phenomena is
believed to have been an extraordinarily heavy rainfall, associated with a hurricane, that
flooded the fieldplots and rendered them temporarily anoxic. The microbial ATP levels
had recovered well by day 37 for all fieldplots.This good recovery was probably
enhanced by renewed tillage of all plots at day 33. Further  activity fluctuations, until day
102 are also believed to have been caused by different aeration conditions induced by
tillage, temperature fluctuations and periodic frost
        A third waste application followed on day 102, when both groups were re-
loaded with 2.5% of buried waste. Sampling took place after the waste application, and
counts at this point were lower than those of the control plot No significant changes
were seen over the following four days, a period believed to be a task of adaptation to
the new carbon sources.  At day 113 however, significant increases in activity were
observed.  A final waste application of 2.5% buried waste was applied to group n at day
156 of the experiment Even though sampling took place after waste application, the
microbial activity for group n, P14 and P15 were significantly higher than that of the
other experimental plots. This unexpected phenomena was probably due to the short
time period between application, sampling and tillage of these two plots on that day,
while no activities were carried out for the other field plots. At the end of the experiment
(day 177-184), group n plots contained significantly higher activity than the control and
group I plots. The latter probably were nutrient depleted or deficient of a bioavailable
carbon source, while the former still were growing on the toxicant carbon source.
3. Microbial diversity
       Microbial diversity is presented in Fig. 26-28. Plating studies for bacteria and
actinomycetes reflect the ATP biocounts for day 23. The biomass throughout the
experiment lay in the range of 107organisms/g wet weight soil which is comparable to a
rich agricultural soil No further variations in biomass were observed between day 30
and day 37. At day 51, there was a reduction in microbial activity, but a high biomass
                                         57

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   Fig. 26: Microbial diversity of field plots, day 23-58

       12
o
(A
I
O)
                                                        D23
                                                        D30
                                                        D37
             PL1     PL2     PL3     PL4

                          RELD PLOT
                                            PL5
o
(0
O>

5
T"
X

*
•B

d
CO
       12-1
       10-
                                                         D51
                                                        D5S
            PL1     PL2     PL3     PL4     PIS

                         RELD PLOT
                              58

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 Fig. 27: Microbial diversity of field plots, day 102-141

       12

a>

o

|
x
 •

<
•8
CO
10


 8


 6


 4
                    PL2     PL3     PL4

                         FIELD PLOT
                                           PL5
                                                        D1Q6
                                                        D 113
                                                        D102
 8
 i
             PL1      PL2     PL3     PL4

                          RELD PLOT
                            59

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 Fig. 28: Microbial diversity of field plots, day 170-184

       12
o
at
•J

"55

o>

5
•0

tj
00
10 J
                                                I  D170
                                                I  D177
                                                I.D184
             PL1      PL2     PL3     PL4     PL5


                          FIELD PLOT
                              60

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was indicated by SPC. It is believed, that the biocounts using SPC could be higher than
expected due to the suggested presence of some spore-forming species. Those, once
inoculated on the Petri dishes having a more readily available carbon source, germinate
and grow out in high numbers, but are relatively inactive in the ecosystem. After the
third waste application at day 156, all experimental fieldplots invariably contained more
biomass than the control plotThis was probably due to the better nutrient supply
provided in the wastes contained in these plots.
4. Microtox™ and TOC analysis
       The Microtox™ toxicity results for the WSF fractions of the 0-6 inch soil core
samples are given in Fig. 29. Day 00 indicates analysis of plots before any treatments.
Group I and Group n received waste loadings on day zero of 4% loading rates of buried
waste and lagoon waste, respectively. Samples were collected after waste incorporation.
Group n WSF showed highest toxic responses by the test organisms, indicating this
waste to be the most toxic. The T.T.5 values for Group I were lower than for Group II.
This level of higher relative toxicity for the Group n samples was expected, since more
toxic waste constituents had been found in lagoon waste than in buried waste. Task n
mesocosm studies also confirmed this phenomena. After a period of 45 days, both
groups showed conditions similar to the initial ones (Day 00), which led to the
conclusion, that substantial waste reduction took place during this time period.
Continuing decreases in LL5 values for group I was observed until day 86. At day  102,
the significant increase is in response to a second reloading of buried waste incorporated
that day before sampling. This LT.5 observation would predict another decrease in WSF
toxicity until day 184. The deviation from the expected pattern was believed to be due to
the presence of toxic intermediates and/or partially solubilizcd metals during this time
period. A toxic effect has been observed for the group I samples at day 184 to a depth of
18 inches. This indicated downward migration of soluble constituents, and possible
inhibition of soil degradation processes. Since the control samples fell in the transition
                                        61

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                               Fig. 29:    Microtox Toxiclty Results of Reid
                                            Samples (0"-6")
                  70 j

                  60 ••

                  50 ..

LL5 (Relative Mean  40
    % Loss of
Bioluminescence In
  WSF Samples)    30 +
  4% B before sampling
    A
4% 8 before sampling

        I
2.5% B before sampling

           I
                     B « buried waste

                    L» lagoon waste
                                                    62

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range over the same time period, other intermediates or toxic substances may have been
present, which contributed to this toxic response by the bacteria. Microtox™ toxicity
results for group n followed the same pattern as group one, except there were a total of
four alternate waste applications for group n while there were only two waste
applications for group I.
       Reduction in LL5 light outputs was observed over the first 45 days of the
experiment, while the subsequent increase in WSF toxicity indicated potential
overloading of the plots. On day 184, downward migration to a depth of 18 inches was
evident, while no toxic effects were observed earlier at this depth. This limited
downward movement is still within the depth of acceptable treatment (Matthews,
personnel communication).
       TOG data was provided by West Paine Laboratories, Baton Rouge, LA. TOC
analysis of day 149 and day 184 are depicted in Fig. 30 and 31. Day 184 confirmed
Microtox™ data in that no significant migration of materials was noted with increasing
depth of 18 or 24 inches. With the exception of PL4, where limited downward migration
was evident in a depth of 12 inches, all plots followed the pattern of the control plot
indicating very little vertical migration. This limited migration of PL4 was also
confirmed by GC/MS data. Both analyses indicated no carbon accumulation with
increasing depth. These results  demonstrated the usefulness of the TOC assay in
monitoring vertical migration of organic carbons in the soil environment However, this
assay should not be used alone, to estimate soil migration patterns.
5. Soil moisture andpH
Tables 9 andlO depict the soil moisture conditions and pH measurements for all the
field plots over the total 184 days of the experiment for the 0-6 inch-depth intervals. pH
values were mostly neutral or slightly alkaline, a range which normally favors microbial
growth of bacteria. The pH values did not drop nor rise significantly thus imposing little
effect on the soil processes.
                                         63

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                       Fig. 30:   TOO analysis for Day
                                      149
TOO (mg/Vg C)
            25000 r
            20000 • •
            15000- •
            10000"
            5000- •
                    fl.1
P12
PL3

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                                 Fig. 31:    TOC analysis for Day 184
TOC (mg/kg C)
               25000 T
               20000 •
               15000 •
               10000
                5000 A
                                       12                 18
                                            Depth in inches
24
                                        65

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TABLE 9:  % Moisture of Fieldplots (0-6" depth)
                                                   Time in days
Mesocosm    03          10          23          30         51          58         65        102
PL1
PL2
PL3
PL4
PL5
(cont.)

PL1
PL2
PL3
PL4
PL5
17.93
18.61
18.96
22.42
21.55

106
28.53
22.99
23.99
21.45
23.35
26.51
28.76
31.40
31.03
24.42

113
22.56
22.80
22.80
20.33
22.17
23.54
25.42
23.06
21.86
23.38

127
20.74
19.13
17.33
16.66
18.81
26.26
25.33
25.79
28.31
24.77

134
15.55
17.01
17.91
15.60
18.29
20.88
23.50
21.41
22.95
23.82

141
14.44
19.23
16.44
16.45
14.78
18.39
20.45
19.73
24.20
19.48

156
23.42
22.54
22.41
22.40
22.48
19.29
17.84
12.99
20.08
22.44

170
17.36
19.48
18.63
19.81
17.77
26.37
26.99
24.53
23.37
23.92

177
17.23
19.02
15.83
17.43
19.78
18.4
19.8
24.4
24.1
23.3

184
17.1
15.2
18.8
15.5
18.9

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TABLE 10:  pH Profiles of Field Plots  (0-6"  depth)                 Time in days

Fieldplot          0           3            10          23         30          44          51          58
PL1
PL2
PL3
PIA
PL5
(cont.)
Fieldplot
PL1
PL2
PL3
PL4
PL5






65
7.4
7.3
7.3
6.9
6.8
8.08
8.23
8.45
8.16
8.43

102
7.68
7.71
7.25
7.59
7.51
6.17
6.16
6.52
6.44
6.22

106
7.26
7.33
7.34
7.24
7.27
7.00
7.20
7.60
7.50
7.30

113
7.60
7.42
7.65
7.50
7.70






127
7.50
7.50
7.70
7.60
7.50
7.00
7.50
7.80
7.40
7.35

134
7.7
7.7
7.7
7.7
7.5
7.50
7.65
8.02
8.05
7.91

141
7.3
7.5
7.5
7.6
7.5






156
7.9
7.8
7.6
7.8
8.6

.
-
7.61
7.72

170
8.2
8.2
8.4
8.1
8.3
8.21
7.85
7.75
6.71
7.11

177
6.8
6.8
6.8
6.7
6.8
8.25
7.00
7.00
6.90
6.90

184
7.8
8.0
8.1
8.2
7.9
      * pH measurements: Ig soil in 99ml distilled deionized H20
       verified by measuring with various pH instruments.

-------
The moisture ranged from almost 13%, as the lowest recorded value, up to 31 %.
Moisture contents correlated with microbial activity data. Moisture was not a limiting
factor in the field studies since it never became marginal, however only in combination
with good aeration can it favor microbial growth.
6. Plant Biomass Determination
       Since one of the ultimate goals of in situ biological treatment consists of the
establishment of a vegetative cover over the treated areas, it was of special interest to
measure the amount of plant biomass after some time. Four months after the last
experimental treatments at the field plots had been carried out (August 13 -17,1986), the
above ground plant biomass was determined. Varieties of plants indigenous to the area
were growing in the field plots. There was a very gradual, but defined increase in plant
biomass from group n, to group I, to the control field plot The control plot, which had
never been loaded with any toxicants, had substantially more plant biomass than the other
groups. Some individual plants in this plot  reached a height of five feet, seven inches.
Plant height in the other plots was generally lower. However, group I plots (PL2, PL3)
also contained individual plants up to five feet tall.. Only small grasses, growing
sporadically were found in the group two plots (PL4, PL5). These two plots also showed
evidence of water logging toward the western edge of the plots, while there was  no direct
water logging seen in the other plots. Fig. 32 shows the g plant dry weight /m2.
       These plant biomass results correlate with the toxicity predictions of the
Microtox™ measurements. Both indicated an overloading of these plots with the oily
sludges, resulting in toxic conditions for the micro- as well as the macrofiora. A  factor
contributing to these toxic conditions especially for the group n plots was probably the soil
composition in the plots, which seems to have been too high in clay and silt content, thus
preventing good aeration for maximum microbial activity and favoring water logging.
However, soil composition was acceptable in preventing downward migration of organics.
                                           68

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                                   Fig. 32: Plant biomass
                                determination of field  plots
                600T
                500.
                400--
g plant dry wV m2
field plot surface
                200-
                100-
                        PL1
  PL3
Fleldplot
PL4
PL5
                                             69

-------
       In addition, IT representatives indicated hydraulic overloading of the group II
plots, caused by excessive amounts of water that was not separated from the sludge before
applications. Over saturation with water therefore prevented maximum microbial activity
and rendered degradation by indigenous microbial populations incomplete. Over saturation
with water may have also indirectly caused oxygen-deprivation of plant roots thus
rninimizing plant growth in these plots.
                                          70

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V. Discussion
       Bacteria play the major role in hydrocarbon degradation in soil environments. It is
reported in the literature, that most of the responsible bacterial strains are sensitive to
acidity and only little growth is reported at pH values of below six or even five (1). The
soil pH thus plays an important role in hydrocarbon degradation. Most bacteria have limited
tolerance for acidic conditions in the soil, fungi are more resistent (9). Consequently, the
soil pH often determines the types of microorganisms that assist in hydrocarbon
degradation.  There is evidence that the overall rate of hydrocarbon biodegradation is
higher under slightly alkaline conditions than under acidic conditions (9). The soils tested
in our field studies were neutral or slightly alkaline. Soils in Louisiana are generally more
basic in nature and well buffered, therefore pH did not impose a problem on the microbial
consortium tested in our investigations. The pH range of our tested soils should, indeed,
favor the growth of the mixed bacterial-fungal communities. No pH lower than six was
measured in our experiment, as can be seen in Table  10.
       Abiotic factors, especially temperature and moisture changes contributed to the
relatively great changes in the biological parameters measured. Controlled conditions as
maintained in the laboratory are not achievable in field studies. Since the initial steps of
hydrocarbon biodegradation are oxygen dependant, and the rate of degradation is highest
when aeration is maximized, monitoring and maintenance of oxygenated conditions in the
field are important (9). Aeration of field plot soils was achieved by tillage of the plots and
raking of the surfaces to remove the formed crusts. Large amounts of readily usable
organic substrates, including hydrocarbons, tend to deplete the oxygen reserves of the soil,
especially if small pore spaces or a high degree of water saturation slows oxygen diffusion
(9). Too much moisture in the soil interferes with the availability of oxygen and renders
aerobic microbial metabolic activity marginaL Therefore, it is suggested, that greater tillage
or better mixing methods be applied by the field engineers.
                                            71

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       The oxidation of aliphatic hydrocarbons has been reported to occur in the
temperature range from 0 C to about 55 C (1). Aromatic hydrocarbons are also oxidized at
a wide range of soil temperatures, starting from freezing point and optimizing between 30
C and 40 C (8). In experiments conducted by Dibble and Bartha with a New Jersy soil,
hydrocarbon biodegradation was highest at temperatures above 20 C with no further
increase in rate at 37 C. The generally warm local temperatures of the southern Louisiana
region should therefore favor the conditions for landfarming and ISBT for hazardous
waste sites in the region.
       To eliminate the interference of other readily available carbon sources in the soil,
that could delay toxicant-carbon uptake, the carbon content of the soil was determined
before waste application. The determination of the % carbon content of the soil (Table 5)
was a necessary step to minimize competition for other carbonaceous substrates, which
could probably lead to preferential utilization by the indigenous microflora. The design of
our experiment did not give insight in the direct fate of the hydrocarbons, if they were
metabolized by the microorganisms and used for growth directly or first cometabolized in
some form. Cometabolism is defined as the metabolism of a compound by a
microorganism, mostly fungi, that the cell cannot use as an energy source or source of
growth (1). The ecological significance of hydrocarbon cometabolism is still uncertain, but
it is known that many microorganisms metabolize aliphatic hydrocarbons that they cannot
use as carbon source for growth, but could serve other microorganisms in some form as a
source of growth. (1). These findings could help to explain the formation of the many
organic intermediates formed in the soiL It is therefore very important to maintain a diverse
microbial consortium in the soil habitat
       Using SPC method, the microbial counts recorded in our experiment for both
laboratory mesocosms and field verification studies were relatively  high, but  generally
reflected the degradation rates. SPC counts from 10^-108 are reported as normal for a good
                                             72

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agricultural soil. Of course, this depends on physical and chemical characteristics of the
soils (1). A possible explanation for these recorded high counts, not associated with
substrate utilization, could lie in the fact, that some of the indigenous microbial strains were
spore-forming organisms, that germinated when plated on the petri-dishes. Thus, they
contributed to a higher microbial density on the plate, while, in the natural ecosystem, they
are inactive.Generally, about 50% of a microbial cell consists of carbon, and frequently,
under aerobic conditions, from about 20 to 40 % of the substrate carbon is assimilated, the
rest is released as carbon dioxide.
       The general purpose of the commercial inoculum experiment was to study the
effects of a commercial blend of microorganisms on the rates of hydrocarbon degradation.
Since there was no experiment conducted using a sterile soil/waste mixture, inoculated
with the commercial inoculum only, it is not possible to attribute the exact percentages of
microbial transformation to either the commercial or the indigenous microflora. Only
general trends can be observed. The reason for not sterilizing the soil is found in the
purpose for ISBT, namely to simulate the natural ecological environment as closely as
possible. A situation where soil is sterile would never occur in the real ecosystem and was
therefore not considered. Sterilization could also affect the nature of the soil.  Since
toxicants often react differently in the presence of one or more other toxicants, no general
behavioral guidelines can be followed and therefore multi-species x multi-toxicant
interactions were the preferred study goal. An artifact in the use of commercially available
inocula may have been, that these inocula are often supplied as freeze dried organisms in a
bran of sawdust containing ample nutrients in the form of fertilizer. During resuspension
with water and application to the assigned soil plots, this fertilizer probably stimulated the
growth of the indigenous microflora just as well as that of the commercial organisms. Due
to the so increased overall biomass, there may be a faster overall initial degradation rate of
the toxicants. No specific and exact statements, however, could be made of what kind of
microorganisms were degrading what part of the waste. Elucidation into the different
                                            73

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 individual biochemical processes could be achieved, and extrapolations on the
 detoxification efficiency of just the commercial cultures could not be made. Therefore,
 these results were not useful in explaining the biochemical reactions associated with waste
 degradation in the soil environment. The benefit of using commercial inocula consisted of
 the enhanced rate of degradation and probably the decreased lag phase. This benefit would
 be especially helpful when time is a limiting factor.
       The interpretations of the GC data as well as the HPLC data were rendered more
 difficult, because all chromatograms showed an increased baseline with time of toxicant
 exposure with a substantial amount of unresolved compounds under this baseline. Even
 though the current methods of analysis are quite sophisticated, they were not capable of
 resolving all the aromatic components into individual compounds. This is partially because
 there were thousands or even tens of thousands of molecules present (24). Many of them
 were microbial intermediates which are too polar in character to be resoved by the
 extraction techniques designed for PAH's. One of the major future goals is to resolve these
 unresolved mixtures and try to increase the extraction efficiency and consequently decrease
 the amount of residuals. Such investigations are presently under way in our laboratory.
       Associated with these interpretations are the aspects of photooxidation
 and volatility of the hydrocarbons. The GC chromatograms do not give any evidence of
 qualitative toxicant disappearance. The kinetics of toxicant disappearance for lower
 molecular weight PAHs, e.g. naphthalene, can not be completely attributed to a microbial
 biotransformation scenario, but may also involve  the high volatility of these compounds.
Moderate abiotic losses of these PAH's due to volatility have been reported (65), but are
 believed to not be substantial. Photooxidation of the PAH's was minimized in the
mesocom tests, by incubating the experimental units, covered, in the dark. Other
unidentified abiotic mechanisms of PAH loss probably occurred Additional investigations
into identifying intermediate abiotic transformation processes are under way. In the field
experimental studies, there was no covering of the experimental plots and the amount of
                                          74

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hydrocarbons lost due to photooxidation could not be determined, but only materials in a
very thin layer after each application and tillage are going to be photooxidized . Therefore,
photooxidation was insignificant in direct sunlight However, a few cm of dark soil may
get hot enough to contribute to excessive volatilization.
       In landfarm soil experiments conducted by Bossert et al. (8), analysis of oily
sludge at the conclusion of a 1,280 day laboratory simulation showed F-2 fractions to
have been more rapidly eliminated than F-l fractions. Although parallels can be drawn
with our field experiment, their F-2 fraction constituted only a small portion of the total
hydrocarbons present, 47% of the total hydrocarbons applied (7 applications) was
eliminated in their bioflask study (8). Preliminary data from our field experiments
indicate a net total hydrocarbon removal of 80.34 % for group I and 83.40% for group
II by day 25. The differences in overall microbial performance in the degradation process
of the phase n and phase ffl investigations reported above and those of Bossert et al. (8)
are due to differences in environmental parameters such as temperature, moisture, soil,
microbial diversity/density and other biotic or abiotic factors.
       Microorganisms are generally the first biota exposed to the toxic insults by toxicants
in the soil environment Therefore, they are useful for initial toxicity tests since they are
generally well adapted (37). The Microtox™ toxicity test provides a rapid measurement of
the WSF concentrations in a soil sample at the time of sample collection.  Changes in the
light output from luminescent bacteria (Photobacterium phosphorewri) are monitored in a
photometer when exposed to various concentrations of toxicants (13). It does not provide a
measure of of the potential accumulation of refractory hazardous organics in the waste-soil
matrix.
       The results of the tested field samples indicated limited downward migration of
some organic components within an acceptable range, but generally exhibited patterns of
normal soil functioning (Matthews, personnel communications). This downward leaching
indicated, that the waste reapplications needed to be carefully evaluated in order to prevent
                                             75

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overloading of the field plots (Matthews). Also, it must be emphasized, that there were
potential additive, synergistic and antagonistic interactions among the metabolites and
intermediates formed in the soil samples over time. These interactions generally render the
identification of those constituents, that exhibit the toxic effect on the bacteria quite
difficult. However, the test is well suited to serve as a primary screening test to indicate
whether a sample is non-toxic, toxic or very toxic. These results were especially useful if
correlations and comparisons with other bioassays could be done (13). However, a
chemical's toxicity varies dramatically from one species to another, as has been shown by
experiments done by Liu (37), who tested 6 different cultures of bacteria for the same
variety of toxicants (12 test chemicals). Therefore, our results do not give much evidence
in all the interactions between the different bacterial or fungal species in the soil. The
interactions between the biota and the toxicants are very complicated in the ecosystems and
there are no general patterns to follow (37). The studies  done by Liu demonstrate the
extreme complexity and unpredictability of the biota-toxicant interactions. In light of these
findings, the focus of the ecotoxicological research done in our experiments was to
determine the overall trends of toxicant disappearence under optimal environmental
parameters. Environmental insults and stresses are thus best monitored in multi-species
multi-toxicant experiments.
       Another major problem occured in sample variability. Mixing the soil with the
grease was difficult due to the high viscosity of the sludge. Hence, lack of homogenicity
may have introduced sampling errors. Sample errors were especially problematic in
HPLC analyses, while for the microbial methods, they were compensated by using a
Turrax homogenizer prior to further preparation. Sample variability was smaller in GC
analyses, because composite samples of the replicate mesocosms were analyzed, while
the analyses by HPLC always involved individual samples.
       Parallelling the disappearence of some of the aromatics such as phenanthrene, an
increase in their methylated homologs was observed. These methylated homologs are
                                           76

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VI. Conclusions
       Microorganisms play a key role in the turnover of carbon in nature. Most of the
known metabolic pathways have been derived from controlled laboratory studies with pure
cultures and single substrates. However, this scenario does not reflect real environmental
conditions, and predictions of microbial behavior under field conditions cannot be made.
Therefore, multiple-toxicant, multiple-species interactions were monitored in our
experiments to evaluate the suitability of the hazardous waste site for ISBT.
       Optimal toxicant loading rate windows, determined in screening tests, were shown
to be  acceptable for inducing microbial biotransformation/biodegradation in mesocosm
units and minimizing acute leachate toxicity. In the above studies, all of the compounds
analyzed exhibited decreases in concentration over time, for both laboratory and field tests.
The decreases were mostly attributed to microbial activity by the indigenous soil
microfiora. However, undefined abiotic losses were noted and need to be further studied.
Both waste types, the lagoon and buried wastes, at both loading rates, 2.5% and 4%, were
degraded by the indigenous microfiora. Mutagenicity and carcinogenicity of these
intermediate degradation products need to be evaluated.
       Task n and task HI studies verified microbial degradation and detoxification
processes. It was shown that the indigenous microfiora was adapted to the wastes and
capable of degrading it Substantial hydrocarbon degradation was observed, even though
not all of it could be attributed to microbial activity, as has been discussed earlier.
       In short, it can-be concluded, that:
*      group two (PL4, PL5) plots show indication of waste-overloading toward the end
       of the applications. Microtox™ and plant biomass analyses confirmed these results.
*       soil composition of plots was too high in silt-clay content, causing waterlogging
       and limiting aeration. Soil mixtures for future applications  should contain higher
       sand contents and less clay contents thus preventing the hydrocarbons from binding
       to the clay and reducing waterlogging, but the sand content should be low enough
                                        78

-------
to not promote leaching of soluble compounds. LSU investigators are willing to
confer with IT Corporation engineers on a final soil/sand mixture at such time in
which a general contractor for this project is named (see further recommendations
on hydraulic overloading).
Waste composition used in field plots was not exactly similiar to the wastes tested
in the laboratory (confirmed by GC/MS). This may be due to collection from a
different location at the site thus rendering GC data comparisons more difficult.
A complete GC/ MS profile of hazardous wastes prior to waste application is
mandatory. This will facilitate subsequent post treatment analysis of each waste
application.
Microtox results of WSF toxicity generally exhibited patterns representative of
normal operation of natural soil treatment processes (Matthews), but limited
downward migration of organic constituents has been observed, however no
deeper man the  acceptable treatment zone.
Microtox™ data suggested, that  time periods between sequential reloadings need to
be carefully evaluated and adjusted according to environmental parameters with
special respect to downward leaching of organic constituents.
All compounds analyzed exhibited decreases in concentration over time, but proper
identification and monitoring of potential toxic intermediates formed by the
microbial consortium need to be  further evaluated.
Indications from IT Corporation  engineers, that hydraulic overloading of lagoon
wastes due to excessive amounts of water not separated from the sludge were
recently presented to LSU investigators. This variable, although not mentioned in
previous correspondence represents a significant problem for general waste
application. It is of the same order of magnitude as waste over loading.
                                   79

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       It is recommended that IT Corporation suggest specific engineering improvements
       for waste application so as to minimize anaerobic  conditions during waste
       application due to hydraulic over loading (phase separation of oil and water).
*      Laboratory data indicated that soil mixtures were adaequate from a microbiological
       perspective. Field tests, based on GC/MS and TOC data, indicated that this mixture
       was adaequate, i. e. minimal leachate problems. However, a more complete mixing
       of waste to soil is needed.
       Heavy equipment, specifically modified for waste application efforts is currently
       available. IT Corporation has been advised of potential manufacturers of this
       equipment
*      For large scale waste application, significant quantities of soil'exhibiting minimal
       microbial activity (specifically microbial activity related to hydrocarbon
       degradation) may be used.
       It is recommended that either adapted microbial populations be generated in these
       soils prior to the initial waste application or commercial inocula be used

Under proper management microbial degradation and detoxification of the site is
scientifically verifiable and economically feasable. Post-closure monitoring of
soil and leachate collected from the site is recommended for a time period of 30
years after setup of landtreatment facilities. Closure activities are varied throughout the site
and include more than just biodegradation. Although we have been requested to provide
engineering input on many of these activities, our focus has been strictly associated with
biological closure, L e. the degradation of the waste materials. We choose not to comment
on other aspects of the Phase in Engineering Design Final Report These activities are quite
properly the responsibility of IT Corporation.
                                        80

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believed to be formed by microbial activity. Methyl groups are often converted to
carboxyl groups prior to ring cleavage. In certain compounds, however, the methyl is
not removed before the ring is opened (11). The carboxyl is often, but not always,
removed before ring cleavage. The methoxyl is replaced by a hydroxyl and gives rise to
formaldehyde (1).
                                          77

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                               BIBLIOGRAPHY
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                                 APPENDIX A

 Literature Review on Hydrocarbon Biodcgradation
A. General
       Microbial metabolism of hydrocarbons has been reported in the literature for several
decades. Some of the first investigations date back as early as  1928 , when Gray and
Thornton (46)  first reported soil bacteria capable of decomposing certain aromatic
compounds. In 1941, Bushnell and Haas (46) documented microbial degradation of
certain hydrocarbons. Sisler and Zobell (57), in  1947 used microorganisms of marine
origin  in their experiments to degrade aromatic hydrocarbons. They studied the utilization
of polyaromatic hydrocarbons (PAH's) by mixed cultures of marine bacteria. PAH's were
introduced into seawater cultures adsorbed to ignited sand. The amount of PAH
metabolized by the bacteria was determined by measuring the amount of carbon dioxide
evolved in hydrocarbon oxidation and substracting carbon dioxide produced by the control
cultures. In these experiments, phenanthrene and anthracene were metabolized more
rapidly than naphthalene, benz(a)anthracene, and dibenz(a,h)anthracene. (57).
       In the present decade, it is well known that hydrocarbons are ubiquitous in the
environment and even found in relatively pristine areas. Their sources are of natural as well
as anthropogenic origin. Due to the toxicity, mutagenicity and carcinogenicity that many of
them exhibit after undergoing metabolic activation, hydrocarbons in the environment may
pose a  hazard to the biota and, ultimately, to human health (12). Major environmental fate/
transport mechanisms include:
              evaporation (volatilization)
              photochemical oxidation
              sedimentation
              microbial degradation
                                          87

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1. Definition
       Hydrocarbons are compounds containing carbon and hydrogen.
Aliphatic hydrocarbons are straight or branched chain hydrocarbons of various lengths.
Aliphatic hydrocarbons are contained naturally in waxes and other constituents of plant
tissues as well as in petroleum or petroleum products. Their transformations are
therefore of great significance in the terrestrial carbon cycle (1). The rate of their
decomposition is markedly affected by the length of the hydrocarbon chain (1).
       Aromatic hydrocarbons contain the benzene ring as the parent  hydrocarbon.
Several benzene rings joined together at two or more ring carbons form PAH's. The
toxicity of these molecules is determined by the arrangement and configuration of the
benzene rings. The hydrogens in the aromatic hydrocarbons may or may not be
substituted by a variety of groups. Some of the common substituents are -Cl, chloro; -
Br, bromo; -I, iodo; -NO2, nitro; -NO, nitroso; and -CN, cyano (60).
2. Sources and Formation
       Most of the aromatic hydrocarbons are initially formed by the pyrolysis of
organic material (24). In this process, the temperature determines the type of compound
formed. For example, unsubstituted PAH's are formed at high temperatures (2,000 C)
whereas alkyl-substituted molecules predominate at 80-150 C. The latter temperature
range is usually associated with the formation of petroleum (24). Generally, PAH's are
formed when organic material  containing carbon and hydrogen is subjected to
temperatures exceeding 700 C, which is the case hi pyrolytic processes and with
incomplete combustion (65). Some common sources associated with incomplete
combustion are cigarette smoke, automobile exhaust, and industiral processes.
       The higher the number of joined benzene rings, the lower the rate of degradation.
The very high molecular weight PAH's are less significant in environmental pollution
problems, due to their low volatility and solubility (65). The growth rates of bacteria on
PAH's are directly related to the solubilities of the PAH's (65). Solubility and
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       The mechanisms used by bacteria for the introduction of hydroxyl moieties into
PAH's will depend on whether the substrate contains alkyl substituents (24). The initial
step of aromatic metabolism consists of the modification or removal of substituents on
the benzene rings and the introduction of hydroxyl groups (2). The first metabolites of
unsubstituted PAH's created by bacteria are cis-dihydrodiols, formed by the
incorporation of two atoms of molecular oxygen. Fungi, in contrast, form trans-
dihydrodiols. The enzymes catalyzing these processes are oxygenases,  known as
cytochrome P450 enzyme complexes. Bacteria use dioxygenases, a multicomponent
enzyme system consisting of a flavoprotein, an iron-sulfur protein, and a ferredoxin
(12).
       Although, initial phases of the degradation pathways differ, the reactions proceed
such that only a few common and key intermediates are produced. These few are then
metabolized by essentially similiar processes. Most common of these intermediates are
catechol, protocatechuic acid and to a lesser degree, gentisic acid (1) (Fig. 2). These
three molecules have in common the presence of two hydroxyls. They are then further
oxidized by the five pathways also shown in Fig.2. The products of these reactions,
namely pyruvate, fumarate, and succinate may  then be incorporated in the TCA and other
biochemical cycles (39). The degradation pathways involved are dictated by the site of
cleavage of the aromatic nucleus.
       Naphthalene and its alkylated homologs are among the most water-soluble and
potentially toxic compounds in petroleum. Fig.3 depicts the pathway for the bacterial
oxidation of naphthalene to catechol. It also shows the different pathways  for the
bacterial oxidation of phenanthrene. Oxidation of this compound by fungi  has not been
reported. Special interest has been paid by various researchers to the degradation of
anthracene and its derivatives. These compounds are not acutely toxic, but possess a
structure also found in other carcinogenic PAH's (12). Degradation of anthracene has

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adsorbtivity are the most important physical properties that influence the rate of
transformation. Among the  chemical properties, photochemical reactivity is the most
relevant Tricyclic or larger PAH and related heterocyclic systems show a very reactive
photochemical behavior. They have strong UV adsorption at wavelengths longer than
300 nm (present in solar radiation) and most are readily photooxidized. Photooxidation
plays one of the major roles in the removal of PAH's from the environment (29).
Adsorbed PAH's are photooxidized more rapidly than dissolved PAH's. (65).
       The chemical structures of some of the major aromatic hydrocarbons are shown
in Fig.l. Biological activity  of these compounds  depends on their inherent
stereochemistry. The addition of another benzene ring in a select position of the
compound can result in the formation of a powerful carcinogen, even if the parent
compound does not exhibit much toxicity (10). The reactive sites of the molecules are
called "Bay- regions" (10). Such a Bay region is found in phenanthrene, the simplest
PAH. It resembles that of benz(a)-anthracene and benz(a)pyrene, and is the region
between an angular benzo ring and the rest of the molecule (11). If dihydrodiol-epoxides
are formed in this region, the molecule becomes very biologically reactive and is
suspected to be a ultimate carcinogen. The primary active carcinogen is usually in the
form of a diol epoxide (25). Phenanthrene itself has been shown to be inactive or only
slightly mutagenic in Salmonella assays, but its metabolites may be highly mutagenic and
tumorigenic(ll).
       Historically, it was believed, that a certain area, called the "K region" was related
specifically to the carcinogenic potential of a hydrocarbon compound. Evidence now
suggests mat activation of PAH's is not likely associated with this K region, but rather
occurs via a two step oxidation with the eventual formation of dihydrodiol epoxide (10).
Another portion of the molecule, called the "L region" can increase the carcinogenic
potency of the molecule, if there are substituents on these positions (i.e., the 7 and 12
                                          90

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       Of these, microbial degradation is the area most extensively studied and
commercialized as evidenced by the most recent developments in biotechnology and
genetic engineering. It is a major mechanism for compound removal from sediments and
terrestrial systems. Microbial degradation of aromatic hydrocarbons by bacteria as well
as fungi has been documented in numerous publications. The degradation processes are
generally inversely proportional to the ring size of the respective PAH molecule. The
lower weight PAH's are degraded more rapidly, while molecules with more than three
condensed rings generally do not serve as amenable substrates for microbial growth
(24). Hence the effectiveness of creosote as a wood preservative.
B. Aromatic and Aliphatic Hydrocarbons
       The capacity of microorganisms to grow in a given habitat is determined by their
ability to utilize the nutrients in their surroundings (1). Among the energy sources
available to be utilized by soil heterotrophic microorganisms are cellulose, hemicellulose,
lignin, starch, chitin, sugars, proteins, hydrocarbons and various other compounds (1).
Numerous hydrocarbons, or their derivatives, are naturally synthesized within the soil
while others are added to the soil from various pollution sources. Their mineralization
and formation by the indigenous microfiora are a fundamental component in the general
carbon cycle (2).
       The three major types of microbial metabolism are: fermentation, aerobic
respiration and anaerobic respiration (24). Aerobic respiration plays the most important
role in the transformation of PAH's. Very little anaerobic respiration of PAH's has been
reported. However, anaerobic biodegradation of PAH's has been observed where suited
electron acceptors were supplied (2). Aerobic respiration initially involves the
incorporation of molecular oxygen in the hydrocarbon molecules. The hydrocarbons are
then converted to more oxidized products. Energy produced during these oxidation
processes is partially used in the synthesis of protoplasmic constituents (24).
                                      91

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carbons in benz(a) anthracene) (10). These structure-activity relationships and the
locations of the different regions are shown in Fig.l.
C. Microbial Metabolism of Hydrocarbons
       There are various and controversial scenarios reported in the literature as to the
physical form under which the hydrocarbons are metabolized. Some studies indicate the
presence of large hydrocarbon droplets, others mention micro-drops as small or smaller
than the microbial cells, still others suggest the importance of the water soluble fraction
(WSF) or the utilization of the hydrocarbons in a vapor phase (38). There are also
reports on the importance of emulsifying agents for initiating hydrocarbon utilization.
However, most reported microbial hydrocarbon metabolism processes are intracellular
oxidation processes (38).
       Historically, most of the investigations of PAH biodegradation were concerned
with measuring the amount of CC>2 produced or the fractions of the toxicants (parent
molecule) converted into CO2. In these early studies, CO2  production was the major
focus of attention with little consideration paid to the intermediates formed. Only recently
has it been recognized that there is a need to investigate these metabolites and the ratio of
polar compounds to CO^ The oxygenated polar compounds may be highly mutagenic
and/or accumulative in the aquatic/terrestrial environment and thus be dangerous to living
cells. Recent advances in analytical techniques (such as Thin Layer Chromatography
and/or MS) have revealed the subtle complexity of biotransformation intermediates and
endproducts.
1. Bacterial Transformation (Biotransformation)
       Bacteria are the dominant group involved in the degradation of PAHs. The most
widely occuring species are Pseudomonas, Myobacterium, Acinetobacter, Arthrobacter,
Bacillus andNocardia (1). Bacteria can oxidize PAH'S ranging from the size of benzene
to benzo(a)pyrene. For more highly condensed PAH'S, there is little evidence of
bacterial oxidation (24).
                                         92

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been reported by bacteria as well as fungi and follows the general degradation pattern of
the other PAH's.
2. Fungal Transformation (Biotransformation)
       Many fungi cannot grow with PAH's as a sole source of carbon and energy, but
still have the ability to oxidize these compounds (24). Fungi carry out reactions similar
to mammals in the degradation process. Therefore, fungi are often used as model
systems. Their enzyme systems for the oxidation of PAH's  differs from that of bacteria
(e.g. mono-oxygenases) and is similiar to that of higher organisms. The cytochrome
P450 mono-oxygenase system catalyzes the initial steps in the oxidation of these
lipophilic PAH's. Many fungi add hydroxyls to the ring structures without being able to
open the ring, but subsequent ring opening and cleavage of ether bonds can then be
brought about through cometabolic conversions (1). Cometabolism is defined as the'
metabolism of a compound by a microorganism that the cell is unable to use as an energy
source or source of growth (1).
       An example for a fungal metabolic pathway quite similiar to those in mammalian
systems for the oxidation of naphthalene is given in Fig.4. In contrast to bacteria, fungi
incorporate only one atom of molecular oxygen into naphthalene via a cytochrome P450
mono-oxygenase (12).                   ^^
                                        93

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                                APPENDIX A
Fig. 1:       Structure-activity relationships of PAH's
Fig. 2:       Common intermediates in bacterial oxidation of PAH's
Fig. 3:       Pathway for the bacterial oxidation of naphtalene to catechol
Fig. 4:       Pathway for the fungal oxidation of napthalene
Fig. 5:       Pathway for the bacterial oxidation of phenanthrene
Fig. 6:       Old Inger Site Map
                                            94

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Fig. 1:        Structure-activity relationships of PAH's
source:       Casarett and Doull, J. 1980. Toxicology. The Basic Science of Poisons.
              Sec. edition, edited by J. Doull; C.D. Klaassen and M.O. Amdur.
              Macmillan Publishing Co., Inc. New York. 92.
                             EXAMPLES OF CARCINOGENIC POLYCYCLIC
                      OR HETEROCYCLIC AROMATIC HYDROCARBONS   .
                         Bay region
                                       region
                      Benz[a]anthracene; R » H
                    7,12-DimethylbenzManthracene;
                             R-CH,
                           Benzo[a]pyrene
                                                        L region
                                                   Dibenz[fl,A]anthracene
                                                 H,C
3-MethylchoUnthrcne
                                                     Dibenz[a, AJacridtne
                                                   95

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Fig. 2:       Common intermediates in bacterial oxidation of PAH's
source:       Alexander, M. 1977. Introduction to Soil Microbiology. John Wiley &
             Sons. N.Y. Chichester, Brisbane, Toronto. 219.
                                   HOOC.
                                                         OH
                             -OH

                             -OH
                         COOH
                        Catechol
Protocatechuic
     acid
                                                           H
Gentisic
  acid
                                             96

-------
Fig. 2:        Common intermediates in bacterial oxidation of PAH's
source:        Alexander, M. 1977. Introduction to Soil Microbiology. John Wiley &
              Sons. N.Y. Chichester, Brisbane, Toronto. 219.
               HOO
HOOC
 -2U
COOH
|
(
"• nnw <
^COOH or


Muconic acid


:H
:H
fH v
H\
COOH

COOH
CH „„
1 -"X
HOOC-C /
500H nr CH
or ••
;OOH
CH


V
COOH 1
\CH, )
1 s
CO
i
CH,
Xin,
COOH ,


Acetic
acid
COOH
, 	 ^TH^

COOH
1
CH,
' 'CH,
i
COOH
Succinic
acid

                 Protocatechuic
                    acid
                   COOH
     0-Carboxymuconic acid
                   CHO
                HOO
                  Protocatechuic
                      acid
              >H     CH
                    COH
                    COOH
    2-Hydroxymuconic tcmialdehyde
                    COOH
                            COOH

                            io
            ^•»-——     i  -—   * CWi
          :HO HOOC-C      T „
                    ^.HHOOC-60H

                     CHO
  4-Carboxy-2-hydroxyrnucociic
                                                                    COOH
                                                                  Pyruvic acid

                                                                    Pyruvic acid
                                                                       100H
                                                         CH,
        semialdehyde
COOH
CH
              OH       {
              OCOOH   i
                  -s
                                                          COOH

                                                          CH
                                    Fumaric
                                      acid
                                                          COOH
                                                          }CH,
                                                        -io
                                                          COOH
                                        Maleylpyruvic acid
                                    Pyruvic
                                      acid
                                                      97

-------
Fig. 3:       Pathway for the bacterial oxidation of naphtalene to catechol
source:       Cerniglia, C.E. 1984. Microbial Transformation of Aromatic
             Hydrocarbons, in Petroleum Microbiology, edited by R.M. Atlas.
             Macmillan Publishing Company N.Y., London. 107
                                        H
              (+)-cis-l,2-Dihydroxy-l,2-dihydronaphtha!ene
                        1,2-Dihydroxynaphthalene
                      cU-o-Hydroxy benzol pyruvic acid
                                   I
                                    OH
                           Salicyloldehyde



                                     >H
                             Salicylic acid
                                    'OH

                               Catechol
              META PATHWAY
ORTHO PATHWAY
                                         98

-------
Fig.4:         Pathway for the fungal oxidation of napthalene
source:        Cemiglla, C.E. 1984. Microbial Transformation of Aromatic
               Hydrocarbons. in:Petrolium Microbiology, edited by R.M. Atlas.
               Macmillan Publishing Company N.Y., London.  110.
                                            ... „<
                                            "
                                         $0. *' 'Epoxidi
                                                           2.Naphlhol
                                                           "OM
                        Nophihol.n.   Nophihol.n*  (+MS,2S-dihydr|oxy-l,2.dihydronophlhoUi»»

                                    1,2-oxid*              |M*

                                                        OH
                                                                     •on
                                                   l-Nophlhol    2-Nophlhcl
                                                    •OM
                          oiojsscoi j n==
                        Naphlhalin*  NaphlhoUn*  . .      .  .•   ......    .  ,
                                     12-oxid«   (+)-lS.2S-dihydf|o«y.l,2.dihydronapthol«n«
                                                   l-Nophlhol    2-Naphlhol
                                                99

-------
Fig.5:        Pathway for the bacterial oxidation of phenanthrene
source:       Cemiglia, C.E. 1984. Microbial Transformation of Aromatic
             Hydrocarbons. in:Petrolium Microbiology, edited by R.M. Atlas.
             Macmillan Publishing Company  N.Y., London. 112.
                                          100

-------
Fig. 6:       Old Inger Site Map
source:       D' Appolonia/GDC 1984. Draft Final Report Phase H Feasibility Study.
             Old Inger abandoned hazardous waste site, Darrow, LA. Louisiana
             Department of Environmental Quality, Baton Rouge, LA. D'Appolonia
             Project No. 84-6058  GDC Project No. 84-501. Contract No. 28800-84-01.
             May 1984, fig. 3-12.
                                               101

-------
o
KJ
                                                                                                               LAWOH
                               '•'     .''\   '  '  • '.'•'".'  ;:. MISSISSIPPI  mven   :    •••.'• :  '•   '••'•'••; Vj '.. ,-"•'.    •;;
                                                  •' •                               fiow  ^
                                          IEOEND
                                             A - LACOON X
                                             • - BUN I CO WAITCAMCA
                                             C - IWAMr
                                             0 - 1C SWAMP
                                             E - IOUTMCNN •OUNOAMV
                                             r - MONTAtC
                                             • - TANK I
                                             H - OPMITK ONAINACC
                                                                                                                                         »MIT
                                                                                                          ri«u*c 5.11
                                                                                                      •Asrc ANCA LOCAHOW
                                                                                                     •I* ••!•
                                                                                                    LOUISIANA OCPAHTMCNTOr
                                                                                                    CNVIMOMMCNTAl QUALITT
                                                                                                     •ATOM MOUCt.LOUISIANA
MIC (HMfr
co. w
                           «T-«M.r CO«tT

-------
        APPENDIX  B
Product data by Microbe Masters, Inc.
                103

-------
UMJIKSlfii©
MASTERS, Inc
             'Biotechnology In Action'
                                            PROCULT DATA

                                      MICRO PRO DEIDXIFCER SERIES

                                  Micro Pro Mutant Bacterial Cultures
                                       Hazardous Waste Treatment

      Micro  Pro Detoxifier  Series is  a special blend of bacterial  cultures which were selected and
      nutated  for hazardous waste sites and land farming.  This product line has performed successfully
      in  numerous  hazardous  waste applications  to  degrade  compounds  such  as  styrene,  phenols,
      polynuclear  arcmatics,  amines,  ammonia,  sulfides,  alcohols, and oils and'grease. The product
      series  has proven itself in actual field trials as an economical  tool which can be used to attain
      efficient hazardous waste control.

      FORM:  Dry or liquid bacterial cultures

      PHYSICAL PROPERTIES:

                                     Dry Cultures

                Appearance

                Odor

                Bacteria Count

                Specific Gravity

                pH

                Effective Temperature  50-110° F

                Shelf Life            1 year

                Moisture              15%

      DOSAGES:
         Tan, free flowing powder

         Yeast-like

         8-10 billion/gram

         0.6

         6.0 - 8.5
Liquid Cultures'-

Slightly turbid liquid

Faint grape-lite odor

100 million/ml (nun)

1.0

Neutral

50-110° F

6 months

N/A
     Dosages for  the products will vary according to the specific situation and also the particular
     goals  of  the evaluation. Typical applications would consist of one or two pounds of dry product
     per 100 square feet of waste site.

     Please refer to the reverse side for application instructions.
                                                  104
                   11814 Coursey Blvd. Suite 285, Baton Rouge, LA 7^°<6 (504) 
-------
                                        SOIL fflOAUCMLNTATION
To  ensure  proper  biodegrarhtjon,   ,1  ccmpetible  product  .can  be  rcconttsnclej  by the tschrjcal
department;  or  if so desired, a  lab screening,  or  plot  study,  can  be performed on the particular
soil in question.

Initial chemical concentrations should be found and  napped for  the site.

Selected  product  should  be  applied at the  rate of  1 Ib./lOO square feet of surface area.  This
dosage is effective to about 6-12  inches deep, depending  on soil type.

Product  should  be spread evenly  and the entire  area  thoroughly watered.  The site should be kept
damp (standing veter is not recommended) by  periodic waterings.

Weekly  analyses  for  ammonia  nitrogen,  orthophosphate and pH should be performed.  If nutrient
levels  fall  below  5 -ppm,  they can be supplemented by using 3-5 Ibs. of commercial fertilizer
(8-6-8, 10-10-10, etc.) per 100 feet.

The  above  dosage procedure should be applied weekly  for two applications and biweekly thereafter
as needed to detoxify the area.

Depending  upon  the  soil  penrability,  clay content, etc., periodic  tilling of the soil has been
used  successfully  in  many applications to achieve greater soil  penetration.  Please consult our
technical department for details.

Vhen  possible,  mixing  can be treated by installing  deep wells,  French drains, etc. and allowing
for extended time.
                   «•
Once  a  layer  of  soil has been  detoxified (as  determined by lab analyses), it can be removed or
stored  according  to  EPA  guidelines  and  the dosage procedure can be started again on the newly
exposed toxic laden layer, if applicable.

For additional information or technical  assistance,  please contact:

                                   Technical Service Department
                                       MICROBE MASTERS, INC.
                                         1181A Coursey Blvd.
                                             Suite 285
                                       Eaton Rouge, LA  70316
                                           (504) 665-1903
                                                   105

-------
                      MASTERS, Inc.
       "Biotechnology In Action"
Micro Pro Product

Efetoxifier "A"
   MICH) PRO DETOXIFIER SERIES

     APPiJCATICN SUGGESTIONS

Soil Contamination

Phenol, pentaphenol, cyanides, cyanates, styrene, diviny]
benezene, methycresotinate, trimethyl amine, ethylene
dichloride, dimethylatitino ethanol.
Detoxifier "B"
Polychloriiiated biphenyls, chlorinated hydrocarbons,
pentachlorophenol,  chlorinated hydrocarbons.
Efetoxifier "D1
Basic general chemicals (such as "A" and "B"),  oil, grease,
sulfur compounds.                        --~
Eetoxifier "F1
cacionics,
Eetoxifier "H"
         , anionics and
ethoxylated phenols, detergents.
:tants,
                                 ,  oil (refined and natural), triglycerides,
                            polynuclear arcmatics, napthalenes.
Used to enhance cold weather activity of natural
organisms and other Micro Pro CetoxLfier products.
Effective temperature range is 40-70* F.
                                          106

             11814 Coursey Blvd. Suite 285, Baton Rouge, LA 70816 (504) 665-1903

-------
                  APPENDIX  C
Selected HPLC chromatograms of phase n mesocosm studies,
buried waste (2% O&G) replicates (1B2, 2B2, 3B2), day 0-42
                           107

-------
GDI 82 VI
45.000
mV
-5.000
7182 VIA"
45,000
mV
-5.000
14182 Vl
45.000
raV
-5.000
211B2 VI
45.000
mV
-5.000
351B2 VI
45.000
mv
-5 Oflfl

421B2 VI
45.000
mV
.000
BLANK VI
45.000
raV
-5.000

PTHALATE
45.000
raV
-5.000

AL 19 INJECT # 1 CH 2
^ A J '_^-"-_ /W*»— " *~rm» "' "-I-- - 	 _
111 I' 1
L 3 INJECT # 1 CH 2
AL 6 INJECT # 1 CH 2
-1 ^^> •* * " "iii 	 — r "u" "- ••"'"' ~ i i
AL 10 INJECT ft 1 CH Z
J^^1 " i i •'•" i i i 	 •" ' '" .-i r'~ i i
0-5 10 15 20 25 30
Minutes Press RESUME
AL 10 INJECT # 1 CH 2
i L J ' ' ' ' i . .
AL 7 INJECT * 1 CH 2
i i i i i i
AL 6 INJECT * 1 CH 2
	 r 	 • 	 r - . .
ECT # 1 CH 2
0 5 10 15 20 25 30
Minutes Press RESUME
108

-------
00282 VIAL  2 INJECT tt 1 CH 2
80.000
mV

5.000
7282 VIAL~ 4 INJECT K i CH "I
75,000
raV
0.000
14282 VIAL  7 INJECT « 1 CH 2

75.000
ra9


°'000   'r   „.......
212B2 VIAL 11 INJECT * 1 CH 2

75.000
rav

0.000
        0         5

352B2 VIAL 11 INJECT « 1 CH 2

75.000
raO

0.000
422B2 V

75.000
nV

0.000  	
BLANK VIATTT

75.000
raV

0.000
10        15
    Minutes
20
25        30
       Press RESUME
PTHALATE VIAL  1 INJECT ft 1 CH Z
a-000
-5.000
                            10        IS
                                 Minutes
                    20
          25
          30
       Press RESUME
                                      109

-------
OD3B2 VI
75.000
mv
0.000
73B2 VIA
75.000
mV
0.000
14382 VI
75.000
mV
0.000
213B2 VI
75.000
mv
0.000
353B2 VI
75.000
roV
0.000
423B2 VI
75.000
raV
0.000
BLANK VI
75.000
raV
0.000
112710 V
75.000
mV
0.000

AL 18 INJECT # i CH 2
t>> 	 i i " wj u ir< ^ ^-' i i i
L 5 INJECT it l CH 2
11 Al A -J^V-V/^— ^^^-U^L-^V— ^-u^-^—r—
Vyvjv^
ii*"- i - ' r— niro 	 1 i^-Jf w I I i
AL 8 INJECT « 1 CH 2
n-k»^i • L in i 111 J i-^-^-^ 	 	 — r"^"'^ i i
AL 12 INJECT ft 1 CH 2
|

0 5 10 15 20 25 30
Minutes Press RESUME
AL 12 INJECT ft 1 CH 2
AL 9 idjKil ft 1 CH i
\ _._- -n.r—
A- — 	 ,.. ., 	 , 	 , 	 1 	 ^"^^
AL 6 INJECT ft 1 CH 2
IAL 2 INJECT ft 1 CH 2
i i i i i i -
0 5 10 15 20 25 30
Minutes Press RESUME
110

-------
       EST-PAINE
               we. ,
nn asm »v«. • BATON MOUOC. LA rauo
                              SAMPLE ANALYSES
                                    for
                              IT CORPORATION
                         8124 South. Choctav Drive
                       Baton Rouge, Louisiana  70815
                         Attention:  Ms.  Sue Cange
                             January  21,  1986
       dsl
                                                                    oe

-------
      EST-PAINE
               ixc.
7979 asm AVC. • MTOM HOUOI. m rano
                             IT CORPORATION
                         Baton Rouge, Louisiana
                            January 21, 1986
         Samples  collected  by IT Corporation as documented  by  the
    enclosed  chain-of-custody  forms,  were received  at  West-Paine
    Laboratories, Incorporated on October 11, 1985.  The samples were
    analyzed   according  to   the  Environmental  Protection  Agency
    protocol as referenced below:

    A.  ' Standard   Methods   for  the   Examination  of  Water   and
         Wastewater. 15th Edition, 1980:
         Parameter                          Method
         Chloride                           407B
         Sulfate                            426C
         pH                                 423
    B)   Methods for Chemical Analysis of Water and Wastes.  EPA-600-
         4-79-020, March, 1979:
         Parameter                          Method
         Calcium                            215.1
         Magnesium                          242.1
         Sodium                       '      273.1
    C)   Methods  of Soil Analysis.  Agronomy Part 2,  Chemical  and
         Microbiological Properties, 2nd Edition, 1982:
         Parameter                          Method
         Saturated Paste Extract            10.2
         Cation Exchange Capacity           8-4

-------
      p
      EST-PAINE
               we.
r»rt asm AVI. • IATON nouac. LA TOKO
C)
                             IT CORPORATION
                         Baton Rouge,  Louisiana
                            January  21,  1986
         Methods  of Soil Analysis.   Agronomy Part 2,   Chemical  and
         Microbiological Properties,  2nd Edition,  1982: (Continued)
         Parameter                           Method
         Total Nitrogen                      31-7
         Total Organic Carbon                29-3.5.3
         Total Phosphorous                   24-2
         Sodium Absorption Ratio             10-2
         The results are reported on the following pages.
                                                              ard,  III
       dsl

-------
      •p
      EST-PAINE
      olouz&uied INC. ,
nn GSKI AVI • §ATON MOUOC. LA ntao
                             IT CORPORATION
                         Baton Rouge, Louisiana
                            January 21, 1986
    Sample ID:   0103 Group l. Plot 2 0-6. 10-08-85
    Date Received:  October 11. 19as
    Parameter

    Cation Exchange
    Capacity (meq/lOOg)
    pH (Units)
    Chloride (mg/L Cl)
    Sulfate (mg/L SO4)
    Calcium (mg/L Ca)
    Magnesium (mg/L Mg)
    Sodium (mg/L Na)
    Total Phosphorous
    (mg/kg P)
    Total Nitrogen
    (mg/kg N)
    Total Organic Carbon
    (mg/L C)
            Quality Assurance
   Results    Actual/Found
                   Date/Time
                    Analyst
     8.2
     8.1
    18.5
   <25
    18
     2.8
    25

   160

     3.1

13,700
0.250/0.248
  7.0/7.0
   50/51
 10.0/9.7
0.250/0.250
0.250/0.248
  5.0/5.0

  125/108

  100/81

  200/192
     10-23/FD
10-23/1700/V11
10-25/0800/NB
11-07/1400/DH
     10-23/FD
     10-23/FD
     10-23/FD

11-12/1500/HS

11-13/1700/RC

10-14/1600/NB

-------
      EST -PAINE
             &A INC. i
7*7* GSM A VI • IATON MOUOC. t» 70UO
                             IT CORPORATION
                         Baton Rouge, Louisiana
                            January 21, 1986
    Sample ID:   0109 Group 2. Plot 4 0-6. 10-10-85
    Date Received:  October 11. 1985
    Parameter

    Cation Exchange
    Capacity (meq/lOOg)
    pH (Units)
    Chloride (mg/L Cl)
    Sulfate (ag/L S04)
    Calcium (mg/L Ca)
    Magnesium (mg/L Mg)
    Sodium (mg/L Na)
    Total Phosphorous
    (mg/kg P)
    Total Nitrogen
    (mg/kg M)
    Total Organic Carbon-
          C)
            Quality Assurance
   Results    Actual/Found
                   Date/Time
                    Analyst
    11
     7.9
     5.50
   <25
    23
     3.6
    11

   148

     3.0

16,400
0.250/0.248
  7.0/7.0
   50/51
 10.0/9.7
0.250/0.250
0.250/0.248
  5.0/5.0

  125/108

  100/81

  200/192
     10-23/FD
10-23/1700/VH
10-25/0800/NB
11-07/1400/DH
     10-23/FD
     10-23/FD
     10-23/FD

11-12/1500/MS

11-13/1700/RC

10-14/1600/NB

-------
f>
 EST-PAINE
         IMC. _


  •ATOM MOUOI. LA 70HO
                        IT CORPORATION
                    Baton Rouge, Louisiana
Sanrale ID: 0112 Grouo
Data Received: October
Parameter
Cation Exchange
Capacity (meq/lOOg)
pH (Units)
Chloride (mg/L Cl)
sulfate (mg/L S04)
Calcium (mg/L Ca)
Magnesium (mg/L Kg)
Sodium (mg/L Na)
Total Phosphorous
(mg/kg P)
Total Nitrogen
(mg/Xg N)
Total Organic Carbon
(mg/L C)

January 21, 1986
2. Plot 5 0-6. 10-10-85
11. 1985
Quality Assurance
Results Actual /Found
7.8 0.250/0.248
7.7 7.0/7.0
4.50 50/51
<25 10.0/9.7
22 0.250/0.250
3.6 0.250/0.248
9.8 5.0/5.0
184 125/108
2.4 100/81
24,500 200/192

Date/Time
Analyst
10-23/FD
10-23/1700/VM
10-25/0800/NB
11-07/1400/DH
10-23/FD
10-23/FD
10-23/FD
11-12/1500/MS
11-13/1700/RC
10-14/1600/NB
*
^

-------
  •p
  'EST-PAINE
 laubaia&yu£A we- <
rf in. •
    WON MOUOL LA raao
                         IT CORPORATION
                     Baton Rouge, Louisiana
                        January 21, 1986
Sample ID:   0113 Control. 0-6. 10-09-85
Date Received:  October 11. 1985
Parameter

Cation Exchange
Capacity (meq/lOOg)
pH (Units)
Chloride (mg/L Cl)
Sulfate (mg/L S04)
Calcium (mg/L Ca)
Magnesium (mg/L Kg)
Sodium (mg/L Na)
Total Phosphorous
(mg/kg P)
Total Nitrogen
(mg/kg H)
Total Organic Carbon
(mg/L C)
         Quality Assurance
Results    Actual/Found
                                                           Date/Time
                                                           Analyst
                             9.1
                             7.7
                             6.50
                           <25
                            18
                             3.1
                            11

                           153

                             3.6

                         3,660
            0.250/0.248
              7.0/7.0
               50/51
             10.0/9.7
            0.250/0.250
            0.250/0.248
              5.0/5.0

              125/108

              100/81

              200/192
     10-23/FD
10-23/1700/VM
10-25/0800/NB
11-07/1400/DH
     10-23/FD
     10-23/FD
     10-23/FD

11-12/1500/MS

11-13/1700/RC

10-14/1600/NB

  dsl

-------
       EST-PAINE
T»T» o»w AVI. • BATON flouof. LA row
                               IT CORPORATION
                           Baton  Rouge, Louisiana

                              January 21, 1986
Sample ID: 0106 GrouD
Date Received: October

Parameter
Cation Exchange
Capacity (neq/lOOg)
pH (Units)
Chloride (mg/L Cl)
Sulfate (mg/L S04)
Calcium (mg/L Ca)
Magnesium (mg/L Mg)
Sodium (mg/L Na)
Total Phosphorous
(mg/kg P)
Total Nitrogen
(mg/kg N)
Total Organic Carbon
(ng/L C)
1. Plot 3. 0-6. 10-08-85
11. 1985
Quality Assurance
Results Actual/Found

7.9 0.250/0.248
7.7 7.0/7.0
15.0 50/51
<25 10.0/9.7
20 0.250/0.250
3.9 0.250/0.248
20 5.0/5.0

149 125/108

3.0 100/81

8,810 200/192


Date/Time
Analyst
f
10-23/5D
10-23/1700/VIt
10-25/0800/NB
11-07/1400/DH
10-23/FD
10-23/FD
10-23/FD

11-12/1500/MS

11-13/1700/RC

10-14/1600/NB
       dsl
86-0321

-------
PROJECT NAME

PROJECT NO.	
                                                  CHAIN OF CUSTODY RECORD


                                                 	     SAMPLER (SI SIGNATURE
                                             i i
    SAMPLE
  IOCNTIFICATION
                  SAMPLIM6
                  LOCATION
                               DATE
                              SAMPLED
                                         SAMPLE TYPE
                                            10  AM  OIL
 VOLUME
  TO BE
COLLECTED
  NO OF
CONTAINERS
   TIME
COLLECTION
   BEGAN
   TIME
COLLECTION
COMPLETED
COMMENTS
                                                                               1510
                                                                                          1525
  OlOQ?
                                                                              1515
                                                                                         1533
 01 Oj

Dili
                                                     X
                                                                             uvn

RELINQUISHED BY  NAME

RELINQUISHED BY

RELINQUISHED BY

AUTHORIZATION I
                                                    /HI
                OIWOSAL
                                       DATE /TIME _.

                                       OATE/TlMf _

                                       DATE/TINE 	
    RECEIVED BY NAME

    RECEIVED BY NAME

    RCCEIVED.BY NAME

    DISPOSED BY: 	
                                       DATE / TIME
                                       DATE/TIME.
                                       DATE/TIME .
                                       DATE/TIME.
                                                                                                                       &j£°Q

-------
      'p
      EST-PA1NE
tm OSMI AVL . BATON flOUaC. LA 70120
                                                       INTERNATIONAL
                                                        TECHNOLOGY
                              SAMPLE ANALYSES
                                    for
                              IT CORPORATION
                         8124 South Choctaw Drive
                       Baton Rouge, Louisiana  70815
                         ATTENTION:  Ms. Sue Cange
                             February 17, 1986
    nal
86-0743

-------
      EST-PAINE
          ztartte& INC. <
nn asm AVI. • BATON AOUOC. LA TOUO
                             IT CORPORATION
                         Baton Rouge, Louisiana
                            February 17,  1986
         Samples  collected  by IT Corporation as  documented  by  the
    enclosed  chain-of-custody  forms,  were  received  at  West-Paine
    Laboratories,  Incorporated on the dates  indicated.    The samples
    were  analyzed  according to the  Environmental Protection  Agency
    protocol as referenced below:

    A.   Standard   Methods   for  the    Examination  of  Water   and
         Wastewater.  15th Edition, 1980:
         Parameter                               Method
         Chloride                                407B
         Sulfate                                 426C
         pH                                     423
         Oil & Grease                           503C

    B.   Methods  of  Soil Analysis.  Agronomy Part 2,  Chemical  and
         Microbiological Properties,  2nd Edition,  1982:
         Parameter                               Method
         Saturated Paste Extract                  10.2
         Cation Exchange Capacity                8-4
         Total Nitrogen                          31-7
         Total Organic  Carbon                    29-3.5.3
     nal
86-0743

-------
      PAINE
   tATON NOUOC. LA 70130
                         IT CORPORATION
                     Baton Rouge, Louisiana
                        February 17,  1986
B.   Methods  of Soil  Analysis.  Agronomy Part 2,   Chemical  and
     Microbiological Properties, 2nd Edition,  1982:  Con't...
     Parameter                               Method
     Total Phosphorus                        24-2
     Sodium Absorption Ratio                 10-2

     The results are reported on the following pages.
                      Ill
Manager
nal                                                         86-0743
                                                                     J

-------
      EST-PA1NE
         ottftt^A we, •
 	
r»TIQ8IH AVI. • SATON MOUQf. LA 70190
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             February 17,  1986
    Sample  ID:   Control  Plot «l.  11-25-85 E Q93Qhrs.

    Date Received:   December 23.  1985
    Parameter
    Oil  &  Grease
     (mg/kg)

    Total  Organic
    Carbon (mg/kg C)

    Total  Phosphate
     (mg/kg P)

    Total  Nitrogen
     (mg/kg N)

    Cation Exchange
    Capacity (meq/lOOg)
     Saturated Paste
     Extract:

     Chloride (mg/kg Cl)

     Sulfate (mg/kg SO4)

     Sodium Absorption
     Ratio

     pH (Units)
            Quality Assurance
  Results     Actua1/Found
2,980


3,190


  318


 <200


    9.9
   16.0

   41.1



    2.5

    8.5
                    Date/Time
                     Analyst
 10.0/11.3
  200/191
 0.50/0.52
  100/71
0.250/0.246
 50.0/52.0

 10.0/9.5


  5.0/5.1

  7.0/7.0
12-27/0900/TO
12-26/0900/RC
01-14/1730/GS
01-16/1200/RH
     02-12/VM
01-20/1100/BT

01-19/2000/RC



     02-05/VM

01-17/1200/RC
   nal

-------
      EST-PAINE
         attni06 INC.
nn asm AVI • BATON nouot LA 70120
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             February 17,  1986
    Sample  ID:   Group «1.  Plot *2.  11-25-85 3 0939hrs.

    Date Received:   December 23.  1985
    Parameter
    Oil  &  Grease
     (mg/kg)

    Total  Organic
    Carbon (mg/kg C)

    Total  Phosphate
     (mg/kg P)

    Total  Nitrogen
     (mg/kg N)

    Cation Exchange
    Capacity (meq/lOOg)
     Saturated Paste
     Extract:

     Chloride (mg/kg ci)

     Sulfate (mg/kg SO4)

     Sodium Absorption
     Ratio

     pH (Units)
            Quality Assurance
  Results     Actual/Found
8,540



9,020



  250



  330



    9.2
   10.5

   42.0



    1.2

    8.4
 10.0/11.3
  200/191
 0.50/0.52
  100/71
0.250/0.246
 50.0/52.0

 10.0/9.5



  5.0/5.1

  7.0/7.0
                    Date/Time
                     Analyst
12-27/0900/TO
12-26/0900/RC
01-14/1730/GS
01-16/1200/RH
     02-12/VM
01-20/1100/BT

01-19/2000/RC



     02-05/VM

01-17/1200/RC
  nal
                                 86-0743

-------
      EST-PAINE
             dd urc.
mt asm AVI • SATON nouat. LA TWO
                              IT CORPORATION
                          Baton Rouge,  Louisiana
                             February 17,  1986
    Sample ID:  Group  il.  Plot:  <3.  11-25-85 3 0946hrs.
    Date Received:   December 23.  1985
    Parameter
    Oil  & Grease
     (mg/kg)
    Total Organic
    Carbon  (mg/kg C)
    Total Phosphate
     (mg/kg  P)
    Total Nitrogen
     (mg/kg  N)
    Cation  Exchange
    Capacity (meq/lOOg)
     Saturated Paste
     Extract:
     Chloride  (mg/kg Cl)
     Sulfate (mg/kg SO4)
     Sodium Absorption
     Ratio
     pH (Units)
             Quality Assurance
   Results     Actua1/Found
 7,180

11,200

   259

   240

     9.0
    13.5
    42.4

     1.6
     8.6
 10.0/11.3
  200/191
 0.50/0.52
  100/71
0.250/0.246
 50.0/52.0
 10.0/9.5

  5.0/5.1
  7.0/7.0
                    Date/Time
                     Analyst
12-27/0900/TO
12-26/0900/RC
01-14/1730/GS
01-16/1200/RH
     02-12/VM
01-20/1100/BT
01-19/2000/RC

     02-05/VM
01-17/1200/RC
    nal
                                   86-074-

-------
  •p
   EST-PA1NE
    BATON aouat LA n*»
                          IT CORPORATION
                      Baton Rouge, Louisiana

                         February 17, 1986
 Sample ID:  Group *2. Plot »4. 11-25-85 3 0955hrs.

 Date Received:  December 23. 1985
 Parameter
Oil & Grease
(mg/kg)

Total Organic
Carbon (mg/kg C)

Total Phosphate
(mg/kg P)

Total Nitrogen
(mg/kg N)

Cation Exchange
Capacity (meq/lOOg)
 Saturated Paste
 Extract:

 Chloride (mg/kg Cl)

 Sulfate (mg/kg SO4)

 Sodium Absorption
 Ratio

 pH  (Units)
                                    Quality Assurance
                          Results     Actual/Found
                         5,640


                         6,640


                           268


                           460


                             9.8
                            13.0

                            50.6


                             1.4

                             8.5
 10.0/11.3
  200/191
 0.50/0.52
  100/71
0.250/0.246
 50.0/52.0

 10.0/9.5


  5.0/5.1

  7.0/7.0
                                                                     1
                    Date/Time
                     Analyst
12-27/0900/TO
12-26/0900/RC
01-14/1730/GS
01-16/1200/RH
     02-12/VM
01-20/1100/BT

01-19/2000/RC


     02-05/VM

01-17/1200/RC
nal
                                                          86-0743

-------
      'EST-PAINE
nn asm AVI • BATON ROUGE, i> rano
                              IT CORPORATION
                          Baton Rouge, Louisiana

                             February 17, 1986
    Sample  ID:   Group <2.  Plot »5.  11-25-85 @ 10Q2hrs.

    Date Received:   December 23.  1985
    Parameter
    Oil  &  Grease
     (ing/kg)

    Total  Organic
    Carbon (mg/kg C)

    Total  Phosphate
     (mg/kg P)

    Total  Nitrogen
     (mg/kg N)

    Cation Exchange
    Capacity (meq/lOOg)
     Saturated Paste
     Extract:

     Chloride (mg/kg Cl)

     Sulfate (mg/kg SO4)

     Sodium Absorption
     Ratio

     pH (Units)
             Quality Assurance
   Results     Actual/Found
11,000


10,800


   279


  <200


     7.9
     9.5

    63.6


     1.1

     8.7
                    Date/Time
                     Analyst
 10.0/11.3
  200/191
 0.50/0.52
  100/71
0.250/0.246
12-27/0900/TO
12-26/0900/RC
01-14/1730/GS
01-16/1200/RH
     02-12/VM
 50.0/52.0

 10.0/9.5



  5.0/5.1

  7.0/7.0
01-20/1100/BT

01-19/2000/RC



     02-05/VM

01-17/1200/RC

   nal
                                  86-0743

-------
7*7« GSM AVI • IATON MOUQC. LA 70*30
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             February 17,  1986
    Sample ID:  0286.  0"  -  6"

    Date Received:  January 6.  1986
    Parameter
          Quality Assurance
Results     Actual/Found
                                                            Date/Time
                                                             Analyst
Oil & Grease
(mg/kg)                   610

Total Organic
Carbon (mg/kg C)        2,600

Total Phosphate
(mg/kg P)                 248

Total Nitrogen
(mg/kg N)                 320

Cation Exchange
Capacity  (meq/lOOg)         10
                                             10.0/8.8
                                              200/185
                                             0.50/0.52
                                              100/71
                                            0.250/0.246
                              01-13/1200/TO
                              01-09/0900/BT
                              01-14/1730/GS
                              01-16/1200/RH
                                   02-12/VM
    Saturated  Paste
    Extract:

    Chloride  (mg/kg Cl)

    sulfate (mg/kg SO4)

    Sodium Absorption
    Ratio

    pH (Units)
 20.0

 52.3


   1.6

   8.8
                                         50.0/52.0

                                         10.0/9.5



                                          5.0/5.1

                                          7.0/7.0
01-20/1100/BT

01-19/2000/RC



     02-05/VM

01-14/1145/DT
   nal
                                86-0743

-------
EST-PAIN E
   xttnieA INC.
    JaJxn
nrt asm AVI. • BATON rauai. LA mao
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             February 17, 1986
    Sample  ID:   0287.  6" - 12"

    Date Received:   January 6. 1986
    Parameter
                                   Quality Assurance
                        Results      Actual/Found
                                                            Date/Time
                                                             Analyst
Oil & Grease
(mg/kg)                    660

Total Organic
Carbon (mg/kg C)         3,980

Total Phosphate
(mg/kg P)                  257

Total Nitrogen
(mg/kg N)                 <200

Cation Exchange
Capacity  (meq/lOOg)          7.0
                                             10.0/8.8
                                              200/185
                                             0.50/0.52
                                              100/71
                                            0.250/0.246
                                                      01-13/1200/TO
                                                      01-09/0900/BT
                                                      01-14/1730/GS
                                                      01-16/1200/RH
                                                           02-12/VM
    Saturated Paste
    Extract:

    Chloride  (mg/kg Cl)

    Sulfate (mg/kg SO4)

    Sodium Absorption
    Ratio

    pH (Units)
                          11.0

                          28.0


                           1.5

                           8.8
                                         50.0/52.0

                                         10.0/9.5



                                          5.0/5.1

                                          7.0/7.0
01-20/1100/BT

01-19/2000/RC



     02-05/VM

01-17/1200/RC

   nal
                                                        86-0743

-------
      EST-PAINE
             <&& IKC. i
rmosm AVI. • BATON nouot i>TOKO
                              IT CORPORATION
                          Baton Rouge,  Louisiana
                             February 17,  1986
    Sample  ID:   Q288.  12" - 18"
    Date Received:   January 6.  1986
     Parameter
    Oil  &  Grease
     (mg/kg)
    Total  Organic
    Carbon (mg/kg C)
    Total  Phosphate
     (mg/kg P)
    Total  Nitrogen
     (mg/kg N)
     Cation Exchange
     Capacity (meq/lOOg)
     Saturated Paste
     Extract:
     Chloride (mg/kg Cl)
     Sulfate (ag/kg SO4)
     Sodium Absorption
     Ratio
     pH (Units)
            Quality Assurance
  Results     Actual/Found
  560

1,600

  300

 <200
10.0/8.8

 200/185

0.50/0.52

 100/71
  Cancelled
   27.5
   51.4

  Cancelled
    8.7
50.0/52.0
10.0/9.5


 7.0/7.0

                   Date/Time
                    Analyst
01-13/1200/TO

01-09/0900/BT

01-14/1730/GS

01-16/1200/RH
01-20/1100/BT
01-19/2000/RC


01-14/1145/DT
   nal
                                  86-0743

-------
      EST-PAINE
         
-------
      EST-PAINE
             £A IMC. |
TtTtOSNAVI. • tATON MOUQf. LA TOKO
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             February 17,  1986
     Sample  ID:   0290.  0"  - 6"

     Date Received:   January 6.  1986
    Parameter
          Quality Assurance
Results     Actua1/Found
                   Date/Time
                    Analyst
    Oil  & Grease
     (mg/kg)                    820

    Total Organic
    Carbon  (mg/kg C)         6,530

    Total Phosphate
     (mg/kg  P)                  250

    Total Nitrogen
     (mg/kg  N)                 <200

    Cation  Exchange
    Capacity (meq/lOOg)          8.0
              10.0/8.8
               200/188
              0.50/0.52
               100/71
             0.250/0.246
               01-13/1200/TO
               01-10/1000/RC
               01-14/1730/GS
               01-16/1200/RH
                    02-12/VM
     Saturated Paste
     Extract:

     Chloride (mg/kg Cl)

     Sulfate (mg/kg SO4)

     Sodium Absorption
     Ratio

     pH (Units)
 10.0

 31.1


  1.2

  8.5
50.0/52.0

10.0/9.5


 5.0/5.1

 7.0/7 .0
01-20/1100/BT

01-19/2000/RC



     02-05/VM

01-17/1200/RC
    nal
                                86-0743

-------
     'p
      EST-PAINE
T«T» OSm AVI • tATON HOOOI. UA 70120
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             February 17,  1986
    Sample  ID:   0291.  6"  - 12"

    Date Received:  January 6. 1986
    Parameter
    Oil & Grease
    (mg/kg)

    Total Organic
    Carbon  (mg/kg C)

    Total Phosphate
    (mg/kg  P)

    Total Nitrogen
    (mg/kg  N)

    Cation  Exchange
    Capacity  (meq/lOOg)
    Saturated Paste
    Extract:

    Chloride  (mg/kg Cl)

    Sulfate  (mg/kg SO4)

    Sodium Absorption
    Ratio

    pH  (Units)
            Quality Assurance
  Results     Actual/Found
  640



4,870



  254



  420



    7.9
   13.5

   28.6



    1.3

    8.4
                    Date/Time
                     Analyst
 10.0/8.8



  200/188



 0.50/0.52



  100/71



0.250/0.246
01-13/1200/TO



01-10/1000/RC



01-14/1730/GS



01-16/1200/RH



     02-12/VM
 50.0/52.0

 10.0/9.5



  5.0/5.1

  7.0/7.0
01-20/1100/BT

01-19/2000/RC



     02-05/VM

01-17/1200/RC
   nal
                                 86-0743

-------
      EST-PA1NE
T»T» asm AVI. • BATON nouot. i> TOKO
                              IT CORPORATION
                          Baton Rouge, Louisiana
                             February 17, 1986
     Sample ID:  0292. 12" - 18"
     Date Received:  January 6. 1986
     Parameter
            Quality Assurance
  Results     Actua1/Found
                   Date/Tin
                    Analyst
     Oil & Grease
     (mg/kg)
     Total Organic
     Carbon (mg/kg C)
     Total Phosphate
     (mg/kg P)
     Total Nitrogen
     (mg/kg N)
     Cation Exchange
     Capacity (meq/lOOg)
     Saturated Paste
     Extract:
     Chloride (mg/kg Cl)
     Sulfate (mg/kg SO4)
     Sodium Absorption
     Ratio
     pH  (Units)
1,940

5,020

  275

 <200

 Cancelled
   16.5
   41.6

 Cancelled
    8.6
10.0/8.8

 200/188

0.50/0.52

 100/71
50.0/52.0
10.0/9.5


 7.0/7.0
01-13/1200/1

01-10/1000/F

01-14/1730/G

01-16/1200/E
01-20/1100/E
01-19/2000/F


01-17/1200/1
    nal
                                 86-0743

-------
     'p
      EST-PAINE
7(79 05*1 AVI. • BATON ROUGE. LA 70620
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             February 17,  1986
    Sample ID:   0293.  18"  - 24"

    Date Received:  January 6.  1986
    Parameter
    Oil & Grease
     (mg/kg)

    Total Organic
    Carbon  (mg/kg  C)

    Total Phosphate
     (mg/kg  P)

    Total Nitrogen
     (mg/kg  N)

    Cation  Exchange
    Capacity  (meq/lOOg)
    Saturated  Paste
    Extract:

    Chloride  (mg/kg  Cl)

    Sulfate  (mg/kg SO4)

    Sodium Absorption
    Ratio

    pH  (Units)
            Quality Assurance
  Results     Actual/Found
                   Date/Time
                    Analyst
1,970


4,510


  259


 <200


 Cancelled
10.0/8.8


 200/188



0.50/0.52


 100/71
01-13/1200/TO



01-10/1000/RC



01-14/1730/GS



01-16/1200/RH
   20.0

   36.1


 Cancelled

    8.4
50.0/52.0

10.0/9.5





 7.0/7.0
01-20/1100/BT

01-19/2000/RC





01-17/1200/RC
   nal
                                  86-0743

-------
      EST-PA1NE
             ea inc.
 _
T»T» asm AVI. • BATON nouoc. LA ntx
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             February 17,  1986
    Sample  ID:   0294.  0" - 6"

    Date Received:   January 6.  1986
    Parameter
     Oil  &  Grease
     (mg/kg)

     Total  Organic
     Carbon (mg/kg C)

     Total  Phosphate
     (mg/kg P)

     Total  Nitrogen
     (mg/kg N)

     Cation Exchange
     Capacity (meq/lOOg)
     Saturated Paste
     Extract:

     Chloride (mg/kg Cl)

     Sulfata (mg/kg SO4)

     Sodium Absorption
     Ratio

     pH (Units)
            Quality Assurance
  Results     Actual/Found
1,460


4,330


  241


 <200


    9.9
   18.0

   46.6



    2.4

    8.7
                    Date/Time
                     Analyst
 10.0/8.8
  200/188
 0.50/0.52
  100/71
0.250/0.246
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
     02-12/VM
 50.0/52.0

 10.0/9.5


  5.0/5.1

  7.0/7.0
01-20/1100/BT

01-19/2000/RC



     02-05/VM

01-17/1200/RC
    nal
                                  86-0743

-------
      EST-PAINE
7*79 GSM AVC. • SATON ROUGE. LA 7WJO
                              IT  CORPORATION
                          Baton Rouge,  Louisiana
                             February 17,  1986
    Sample ID:   0295.  6"  -  12"
    Date Received:  January 6.  1986
    Parameter
          Quality Assurance
Results     Actual/Found
                                                            Date/Time
                                                             Analyst
Oil & Grease
(mg/kg)                 2,550
Total Organic
Carbon (mg/kg C)        3,720
Total Phosphate
(mg/kg P)                 268
Total Nitrogen
(mg/kg N)                <200
Cation Exchange
Capacity  (meq/lOOg)          8.8
                                             10.0/8.8
                                              200/188
                                             0.50/0.52
                                              100/71
                                            0.250/0.246
                             01-13/1200/TO
                              01-10/1000/RC
                              01-14/1730/GS
                              01-16/1200/RH
                                   02-12/VM
     Saturated Paste
     Extract:
     Chloride  (mg/kg Cl)
     Sulfate (mg/kg SO.)
     Sodium Absorption
     Ratio
     pH (Units)
 19.0
 44.3

  1.5
  8.6
                                         50.0/52.0
                                         10.0/9.5

                                          5.0/5.1
                                          7.0/7.0
01-20/1100/BT
01-19/2000/RC

     02-05/VM
01-17/1200/RC
    nal
                                86-0743

-------
  EST-PAINE
   MTOM MtMC. LA 7000
                         IT CORPORATION
                     Baton Rouge, Louisiana
                        February 17, 1986
Sample ID:  0296. 12" - 18"
Date Received:  January 6. 1986
Parameter
            Quality Assurance
  Results     Actual/Found
                   Date/Time
                    Analyst
Oil & Grease
(nig/kg)
Total Organic
Carbon  (mg/kg C)
Total Phosphate
(mg/kg  P)
Total Nitrogen
(mg/kg  N)
Cation  Exchange'
Capacity  (meq/lOOg)
Saturated Paste
Extract:
Chloride  (mg/kg el)
Sulfate  (ag/kg SO4)
Sodium Absorption
Ratio
pH  (Units)
1,480

4,450

  248

 <200

 Cancelled
   12.5
   31.1

 Cancelled
    8.2
10.0/8.8

 200/188

0.50/0.52

 100/71
50.0/52.0
10.0/9.5


 7.0/7.0
01-13/1200/TO

01-10/1000/RC

01-14/1730/GS

01-16/1200/RH
01-20/1100/BT
01-19/2000/RC


01-17/1200/RC
nal
                                   86-0743

-------
W9 asm AVC. • IATON nouac. LA TOUO
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             February 17,  1986
    Sample ID:  0297.  18"  -  24"

    Date Received:  January  6. 1986
    Parameter
    Oil & Grease
    (mg/kg)

    Total Organic
    Carbon  (mg/kg C)

    Total Phosphate
    (mg/kg  P)

    Total Nitrogen
    (mg/kg  N)

    Cation  Exchange
    Capacity  (meq/lOOg)
    Saturated Paste
    Extract:

    Chloride (mg/kg Cl)

    Sulfate (mg/kg SO.)

    Sodium Absorption
    Ratio

    pH (Units)
            Quality Assurance
  Results     Actual/Found
1,330



3,720



  241



 <200



 Cancelled.
10.0/8.8


 200/188



0.50/0.52


 100/71
   18.0

   56.2


 Cancelled

    8.5
50.0/52.0

10.0/9.5





 7.0/7.0
                   Date/Time
                    Analyst
01-13/1200/TO



01-10/1000/RC



01-14/1730/GS



01-16/1200/RH
01-20/1100/BT

01-19/2000/RC





01-17/1200/RC
   nal
                                  86-0743

-------
TfT* (MM AVi. • BATON MOUOC. LA 7M20
                              IT CORPORATION
                          Baton Rouge, Louisiana
                             February 17, 1986
     Sample ID:   0298.  0" - 6"

     Date Received:   January 6. 1986
     Parameter
Oil & Grease
(mg/kg)

Total Organic
Carbon (mg/kg C)

Total Phosphate
(mg/kg P)

Total Nitrogen
(mg/kg N)

Cation Exchange
Capacity  (meq/lOOg)
     Saturated Paste
     Extract:

     Chloride (mg/kg Cl)

     Sulfate (mg/kg SO4)

     Sodium Absorption
     Ratio

     pH (Units)
                                    Quality Assurance
                          Results     Actual/Found
5,420


9,500


  268


 <200


    4.4
13.0

44.6


 1.4

 8.5
                                             10.0/8.8
                                              200/188
                                             0.50/0.52
                                              100/71
                                            0.250/0.246
                                         50.0/52.0

                                         10.0/9.5


                                          5.0/5.1

                                          7.0/7.0
                                Date/Time
                                 Analyst
                            01-13/1200/TO
                            01-10/1000/RC
                            01-14/1730/GS
                            01-16/1200/RH
                                  02-12/VM
                                01-20/1100/BT

                                01-19/2000/RC


                                     02-05/VM

                                01-17/1200/RC

   nal
                              86-0743

-------
      EST-PAINE
Ttrt OSMAVI. • iATON BOUOt LA TOiJO
                              IT CORPORATION
                          Baton Rouge, Louisiana
                             February 17, 1986
     Sample ID:   0299.  6" - 12"
     Date Received:   January 6. 1986
     Parameter
     Oil & Grease
     (mg/kg)
     Total Organic
     Carbon (mg/kg C)
     Total Phosphate
     (mg/kg P)
     Total Nitrogen
     (mg/kg N)
     Cation Exchange
     Capacity (meq/lOOg)
     Saturated Paste
     Extract:
     Chloride (mg/kg Cl)
     Sulfate (mg/kg SO4)
     Sodium Absorption
     Ratio
     pH (Units)
            Quality Assurance
  Results     Actual/Found
  630

4,690

  225

1,390

    9.5
   22.0
   53.0

    1.5
    8.6
                    Date/Time
                     Analyst
 10.0/8.8
  200/188
 0.50/0.52
  100/71
0.250/0.246
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
     02-12/VM
 50.0/52.0
 10.0/9.5

  5.0/5.1
  7.0/7.0
01-20/1100/BT
01-19/2000/RC

     02-05/VM
01-14/1615/DT
   nal
                                 86-0743

-------
      •P
      EST-PAINE
nn asm AVI. • BATON nouoc LA ratio
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             February 17, 1986
     Sample  ID:   0300.  12" - 18"

     Date  Received:   January 6. 1986
     Parameter
            Quality Assurance
  Results     Actua1/Found
                   Date/Time
                    Analyst
     Oil  &  Grease
     (mg/kg)

     Total  Organic
     carbon (mg/kg C)

     Total  Phosphate
     (mg/kg P)

     Total  Nitrogen
     (mg/kg N)

     Cation Exchange
     Capacity (meq/lOOg)
     Saturated Paste
     Extract:

     Chloride (mg/kg Cl)

     Sulfate (mg/kg SO4)

     Sodium Absorption
     Ratio

     pH (Units)
  410


3,720


  284


 <200


 Cancelled
    9.5

   23.5


 Cancelled

    8.6
10.0/8.8



 200/188



0.50/0.52



 100/71
50.0/52.0

10.0/9.5





 7.0/7.0
01-13/1200/TO



01-10/1000/RC



01-14/1730/GS



01-16/1200/RH
01-20/1100/BT

01-19/2000/RC





01-14/1615/DT
    nal
                                  86-0743

-------
      EST-PAINE
TfTtOSm AVl • BATON NOUQC. UA 7IM20
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             February 17,  1986
    Sample  ID:   0301.  18"  - 24"

    Date Received:   January 6.  1986
    Parameter
            Quality Assurance
  Results     Actual/Found
                   Date/Time
                    Analyst
    Oil & Grease
     (mg/kg)

    Total Organic
    Carbon  (mg/kg C)

    Total Phosphate
     (mg/kg  P)

    Total Nitrogen
     (mg/kg  N)

    Cation  Exchange
    Capacity (meg/lOOg)
     Saturated Paste
     Extract:

     Chloride (ag/kg Cl)

     Sulfate (mg/kg SO4)

     Sodium Absorption
     Ratio

     pH (Units)
  550


4,100


  257


 <200


 Cancelled
   22.0

   39.3


 Cancelled

    8.7
10.0/8.8



 200/188



0.50/0.52



 100/71
50.0/52.0

10.0/9.5





 7.0/7.0
01-13/1200/TO



01-10/1000/RC



01-14/1730/GS



01-16/1200/RH
01-20/1100/BT

01-19/2000/RC





01-14/1145/DT
    nal
                                  '86-0743

-------
      •p
      EST-PAINE
         a&nteA INC.
 _
rVTfOSMAVI. • BATON MOUGC. LA 7IM10
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             February 17,  1986
    Sample  ID:   0302.  0" - 6"

    Date Received:   January 6.  1986
    Parameter
    Oil  & Grease
     (mg/kg)

    Total Organic
    Carbon  (mg/kg C)

    Total Phosphate
     (mg/kg  P)

    Total Nitrogen
     (mg/kg  N)

    Cation  Exchange
    Capacity (meq/lOOg)
     Saturated Paste
     Extract:

     Chloride  (mg/kg Cl)

     Sulfate (mg/kg SO4)

     Sodium Absorption
     Ratio

     pH (Units)
             Quality Assurance
   Results     Actua1/Found
 6,330


10,900



   252


   300
     8.5
    40.0

    67.7


     1.3

     8.8
                    Date/Time
                     Analyst
 10.0/8.8
  200/188
 0.50/0.52
  100/71
0.250/0.246
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
     02-12/VM
 50.0/52.0

 10.0/9.5


  5.0/5.1

  7.0/7.0
01-20/1100/BT

01-19/2000/RC



      02-05/VM

01-14/1615/DT
    nal
                                                               86-0743

-------
        EST-PAINE
rrr» asm AVI. • IATON nouat, LA TOMB
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Samnle ID: 0303. 6" - 12"
Date Received: January 6. 1986

Quality Assurance
Parameter Results Actual /Found
Oil & Grease
(mg/kg) 2,910 10.0/8.8
Total Organic
Carbon (mg/kg C) 4,450 200/188
Total Phosphate
(mg/kg P) 266 0.50/0.52
Total Nitrogen
(mg/kg N) <200 . 100/71
Cation Exchange
Capacity (meq/lOOg) 10 0.250/0.246
Saturated Paste
Extract:
Chloride (mg/kg Cl) 25.0 50.0/52.0
Sulfate (mg/kg SO4) 29.9 10.0/9.5
Sodium Absorption
Ratio 1.7 5.0/5.1
pH (Units) 8.3 7.0/7.0


Date/Time
Analyst
c
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
02-12/VM

01-20/1100/BT
01-19/2000/RC
02-05/VM
01-14/1615/DT
     nal
86-0743

-------
      EST-PA1NE
im osm AVI • BATON aouaf. LA TOKO
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             February 17,  1986
    Sample ID:  0304.  12"  - 18"

    Date Received:  January 6. 1986
    Parameter
            Quality Assurance
  Results     Actual/Found
                   Date/Time
                    Analyst
    Oil & Grease
    (mg/kg)

    Total Organic
    Carbon  (mg/kg C)

    Total Phosphate
    (mg/kg  P)

    Total Nitrogen
    (mg/kg  N)

    Cation  Exchange
    Capacity  (meq/lOOg)
    Saturated Paste
    Extract:

    Chloride  (mg/kg  ci)

    Sulfate  (mg/kg SO4)

    Sodium Absorption
    Ratio

    pH  (Units)
  580


3,690


  257


  230


 Cancelled
   30.0

   45.0


 Cancelled

    8.7
10.0/8.8


 200/188


0.50/0.52


 100/71
50.0/52.0

10.0/9.5





 7.0/7.0
01-13/1200/TO



01-10/1000/RC



01-14/1730/GS



01-16/1200/RH
01-20/1100/BT

01-19/2000/RC





01-14/1615/DT
   nal
                                  86-0743

-------
      EST-PAINE
rrrt asm AVC. • BATON nouoi. LA TOUO

                              IT CORPORATION
                         Baton Rouge,  Louisiana

                            February  17,  1986
    Sample ID:  0305. 18" -  24"

    Date Received:  January  6. 1986
    Parameter
    Oil & Grease
    (mg/kg)

    Total Organic
    Carbon  (mg/kg C)

    Total Phosphate
    (mg/kg  P)

    Total Nitrogen
    (mg/kg  N)

    Cation  Exchange
    Capacity  (meq/lOOg)
    Saturated Paste
    Extract:

    Chloride  (mg/kg Cl)

    Sulfate  (mg/kg SO4)

    Sodium Absorption
    Ratio

    pH  (Units)
            Quality Assurance
  Results     Actual/Found
                   Date/Time
                    Analyst
  590


4,630


  289


  240


 Cancelled
10.0/8.8


 200/188


0.50/0.52


 100/71
01-13/1200/TO



01-10/1000/RC



01-14/1730/GS



01-16/1200/RH
   18.0

   53.2

     *

 Cancelled

    8.6
50.0/52.0

10.0/9.5




 7.0/7.0
01-20/1100/BT

01-19/2000/RC





01-14/1615/DT
   nal
                                                              86-0743

-------
                                                                                                                      SHEET NO
 PROJECT NAME.

 PROJECT NO_£
         OQ
         33
                    CHAIN  OF CUSTODY  RECORD


                   	     SAMPLERISI SIGNAT
i  OT.  0\
     SAMPLE
  IOCNTIFICATIOM
SAMPLING
LOCATION
  DATE
 SAMPLED
                                            SAMPLE TYPE
                                               10  «m  OIL
  VOLUME
  TO BE
COLLECTED
  NO OF
CONTAINERS
   TIME
COLLECTION
   BEGAN
   TIME
COLLECTION
COMPLETED
                                                                                                   COMMENTS
                                                         IX
                                                                                                                            PMY
                                             IX
                                                                                                                          p uy
                                                                                   <££L
                                                                                             £L
                                                                                         J2LL
                                                    V
RELINQUISHED BY  NAME

RELINQUISHED BY:

RELINQUISHED BY  NAME 	

AUTHORIZATION FOR DISPOSAL.
                      DATE/TIME

                      DATE/TIME

                      DATE/TIME
                                   RECEIVED BY NAME.

                                   RECEIVED BY: NAME .

                                   RECEIVED BY: NAME.

                                   DISPOSED BY: 	
                                                   DATE/TIME.

                                                   DATE/TIME .

                                                   DATE/TIME.

-------
             CHAIN OF CUSTODY RECORD
                            SAMPLER (S) SIGNATUR
PROJECT NAME
PROJECT NO
                                                                                                         TIME
                                                                                                     COLLECTION
                                                                                                   Z  COMPLETED
                                                                        RECEIVED BY  NAME
                                                                        RECEIVED BY: NAME
                                                                        RECEIVED BY  NAME
                                                                        DISPOSED BY:
                                                                                                                          DATE/TIME  ~
DATE/TIME
DATE/TIME
DATE/TIME
RELINQUISHED BY  MAMtSX-hLfl^l'] (_
RELINQUISHED BY:  NAME
RELINQUISHED BY:  NAMC
AUTHORIZATION FOR DISPOSAL

-------

-------
       EST-PAIN E
          atoti^A INC. .
7979 QSM AVl • BATON fWUQC. LA 70620
                               SAMPLE ANALYSES
                                      for
                               IT CORPORATION
                              1150 LeBlanc Road
                        Port Allen, Louisiana  70767
                          Attention:   Ms.  Sue Cange
                                June  18,  1986
   dsl
86-2736

-------
nn asm AVE. • BATON MOUQC. LA TOKO
                             IT CORPORATION
                          Port Allen, Louisiana
                              June 18, 1986
         Samples  collected  by IT Corporation as documented  by  the
    enclosed  chain-of-custody  forms,  were received  at  West-Paine
    Laboratories,  Incorporated on the dates indicated.   The samples
    were  analyzed  according to the Environmental Protection  Agency
    Protocol as referenced below:

    A.   Standard   Methods  for  the  Examination  of  Water   and
         Wastewater. 15th Edition, 1980:
         Parameter                                   Method
         Chloride                                    407B
         Sulfate                                     426C
         pH                                          423
         Oil & Grease                                503C
    B.   Methods  of Soil Analysis.  Agronomy Part 2,  Chemical   and
         Microbiological Properties, 2nd Edition, 1982:
         Parameter                                   Method
         Saturated Paste Extract                     10.2
         Cation Exchange Capacity                    8-4
         Total Nitrogen                              31-7
         Total Organic Carbon                        29-3.5.3
                                                                86-2736

-------
      EST-PA1NE
         4Z&-U641NC. mm
rtraosmAVI. • IATONnouot.LAroaao
                              IT CORPORATION
                           Port Allen, Louisiana
                               June IB, 1986
    B.   Methods   of Soil Analysis.  Agronomy Part 2,   Chemical  and
         Microbiological Properties, 2nd Edition, 1982:
         Parameter                                   Method
         Total  Phosphorus                            24-2
         Sodium Absorption Ratio                     10-2
         The documented results are reported on the  following pages.
                                         Manager
                                                 .  Blanchard, III
    dsl
86-2736

-------
      •p
      EST-PAIN E
      aJtaiatoiteA we.
7978 QSfll AVt • BATON HOUQt LA 70*20
                              IT CORPORATION
                           Port Allen,  Louisiana
                               June 18,  1986
    Sample Identification:   F1197.  6" - 12"
    Date Received:   04-15-86
    Parameter               Result
    Oil & Grease  (mg/kg)     758
    Total Organic Carbon
     (mg/kg C)              4,000
    Total Phosphate
     (mg/kg P)                260
    Total Nitrogen
     (mg/kg N)                340
    Cation Exchange
    Capacity (meq/lOOg)        8.8
    Saturated  Paste
    Extract:
    Chloride (mg/kg Cl)       22.5
    Sulfate  (mg/kg SO4)       37
    Sodium Absorption
    Ratio                      1.4
    pH (Units)                8.7
Quality Assurance
  Actual/Found
    10.0/9.7
     200/192

    0.50/0.47

   5,000/3,890

   0.250/0.250


      50/53
    10.0/9.9

   0.250/0.250
     7.0/7.0

   Date/Time
    Analyst
04-24/0830/TO
04-18/0830/KT

04-30/1500/JS

05-02/0815/KT

     05-08/VM


05-12/1400/RC
05-12/1300/DT

     05-08/VM
05-01/1500/DT
  dsl
                                                                86-2736

-------
      EST-PAINE
Ttft OSNI AVt • BATON ROUOi. LA 70UO
                              IT CORPORATION
                           Port Allen,  Louisiana

                               June 18,  1986
    Sample Identification:   F1199.  18" - 24"

    Date Received:   04-15-86
    Parameter               Result

    Oil & Grease  (mg/kg)     659

    Total Organic Carbon
    (mg/kg C)              4,500

    Total Phosphate
    (mg/kg P)                240

    Total Nitrogen
    (mg/kg N)                400

    Saturated Paste
    Extract:

    Chloride (mg/kg Cl)       14.5

    Sulfate  (mg/kg SO4)       37

    pH (Units)                 8.5
Quality Assurance
  Actual/Found

    10.0/9.7
     200/192


    0.50/0.47


   5,000/3,890





      50/53

    10.0/9.9

     7.0/7.0
   Date/Time
    Analyst

04-24/0830/TO
04-18/0830/KT


04-30/1500/JS


05-02/0815/KT





05-12/1400/RC

05-12/1300/DT

04-28/1400/JS

-------
      EST-PAINE
             eA IKC. (
7*7* QSm AVI. • BATON flOUOC LA 70UO
                              IT  CORPORATION
                          Port Allen,  Louisiana
                              June IS,  1986
    Sample Identification:   F12QO.  0"  - 6"
    Date Received:  04-15-86
    Parameter              Result
    Oil & Grease  (mg/kg)   3,080
    Total Organic Carbon
    (mg/kg C)             10,000
    Total Phosphate
    (mg/kg P)
    Total Nitrogen
    (mg/kg N)
    Cation Exchange
    Capacity  (meq/lOOg)
    Saturated Paste
    Extract:
    Chloride  (mg/kg Cl)
    Sulfate  (mg/kg SO4)
    Sodium Absorption
    Ratio
    pH  (Units)
260
400
  6.2
 21.0
 35

  2.0
  8.9
        Quality Assurance
          Actual/Found
            10.0/11.6
  200/192

 0.50/0.47

5,000/3,890

0.250/0.250


   50/53
 10.0/9.9

0.250/0.250
  7.0/7.0
                    Date/Time
                     Analyst
                 04-28/1400/TO
04-18/0830/KT

04-30/1500/JS

05-02/0815/KT

     05-08/VM


05-12/1400/RC
05-12/1300/DT

     05-08/VM
04-24/1600/DT
       dsl
                                      86-2736

-------
      EST-PAINE
             6d ixc.
7979 OSRI AVC. • BATON ROUGE. LA 70120
                              IT CORPORATION
                           Port Allen,  Louisiana

                               June 18,  1986
    Sample Identification:   F1201.  6"  - 12"

    Date Received:  04-15-86
    Parameter

    Oil & Grease  (nig/kg)

    Total Organic Carbon
    (mg/kg C)

    Total Phosphate
    (mg/kg P)

    Total Nitrogen
    (mg/kg N)

    Cation Exchange
    Capacity  (meq/lOOg)

    Saturated Paste
    Extract:

    Chloride  (mg/kg Cl)

    Sulfate  (mg/kg SO4)

    Sodium Absorption
    Ratio

    pH (Units)
          Quality Assurance
 Result     Actual/Found

  499         10.0/11.6
5,000          200/192


  260         0.50/0.47


  440        5,000/3,890


    7.2      0.250/0.251





   20.0         50/53

   29         10.0/9.9


    1.9      0.250/0.250

    8.8        7.0/7.0
   Date/Time
    Analyst

04-28/1400/TO
04-18/0830/KT


04-30/1500/JS


05-02/0815/KT


     06-05/YM





05-12/1400/RC

05-12/1300/DT


     05-08/VM

04-24/1600/DT

-------
7t79GSfllAVI. • BATON AOUOC. LA 70820
                              IT CORPORATION
                           Port Allen,  Louisiana

                               June 18,  1986
    Sample Identification:   F1202.  12" - 18"

    Date Received:   04-15-86
    Parameter               Result

    Oil & Grease  (rag/kg)     910

    Total Organic Carbon
    (mg/kg C)              4,700

    Total Phosphate
    (mg/kg P)                220

    Total Nitrogen
    (mg/kg N)                360

    Saturated Paste
    Extract:

    Chloride  (mg/kg  Cl)       21.5

    Sulfate  (mg/kg S04)       32

    pH  (Units)                 8.2
Quality Assurance
  Actual/Found

    10.0/8.4
     200/192


    0.50/0.47


   5,000/3,890





      50/53

    10.0/9.9

     7.0/7.0
   Date/Time
    Analyst

06-09/0930/TO
04-18/0830/KT


04-30/1500/JS


05-02/0815/KT





05-12/1400/RC

05-12/1300/DT

04-28/1400/JS
  dsl
                            86-2736

-------
ran asm AVE. • BATON nouoc. LA TOKO
                             IT CORPORATION
                          Port Allen,  Louisiana

                              June  18,  1986
    Sample Identification:  F1204.  0"  -  6"

    Date Received:  04-15-86
    Parameter              Result

    Oil & Grease  (mg/kg)  7,290

    Total Organic Carbon
    (mg/kg C)            12,700
    Total Phosphate
    (mg/kg P)

    Total Nitrogen
    (mg/kg N)

    Cation Exchange
    Capacity  (meq/lOOg)

    Saturated Paste
    Extract:

    Chloride  (mg/kg Cl)

    Sulfate  (mg/kg SO.)

    Sodium Absorption
    Ratio

    pH (Units)
260
480
  8.6
 21.0

 30



  1.4

  8.5
        Quality Assurance
          Actual/Found

            10.0/11.6
  200/192


 0.50/0.47



5,000/3,890


0.250/0.250





   50/53

 10.0/9.9


0.250/0.250

  7.0/7.0
                    Date/Time
                     Analyst

                 04-28/1400/TO
04-18/0830/KT


04-30/1500/JS



05-02/0815/KT



     05-08/VM





05-12/1400/RC

05-12/1300/DT


     05-08/VM

04-28/1400/JS
  dsl

-------
         EST-PAINE
-f
         atotte&wc. ••
7979 QSW AVE. • BATON ROUOE. LA 70620
                                IT CORPORATION
                             Port Allen,  Louisiana
                                 June  18,  1986
       Sample Identification:   F1206.  12"  - 18"
       Date Received:  04-15-86
       Parameter
       Oil & Grease  (mg/kg)
       Total Organic Carbon
       (mg/kg C)
       Total Phosphate
       (mg/kg P)
       Total Nitrogen
       (mg/kg N)
       Saturated Paste
       Extract:
       Chloride  (mg/kg  Cl)
       Sulfate  (mg/kg S04)
       pH  (Units)
                                     Quality Assurance
                            Result     Actual/Found
                             877         10.0/11.6
                           5,500          200/192

                             360         0.50/0.47

                             420        5,000/3,890


                              21.5         50/53
                              27         10.0/9.9
                               8.7        7.0/7.0

   Date/Time
    Analyst
04-28/1400/TO
04-18/0830/KT

04-30/1500/JS

05-02/0815/KT


05-12/1400/RC
05-12/1300/DT
05-01/1700/DT

                                                                    OC

-------
      EST-PA1NE
r<79 asm AVE. • SATON nouae. LA TOKO
                              IT CORPORATION
                           Port Allen, Louisiana
                               June 18, 1986
    Sample  Identification:   F1207. 18" - 24"
    Date Received:   04-15-86
    Parameter
    Oil  &  Grease (rag/kg)
    Total  Organic Carbon
     (mg/kg C)
    Total  Phosphate
     (mg/kg P)
    Total  Nitrogen
     (mg/kg N)
    Saturated Paste
    Extract:  •
    Chloride  (mg/kg Cl)
    Sulfate (mg/kg SO.)
    pH (Units)
          Quality Assurance
 Result     Actual/Found
  950         10.0/8.4
4,500          200/192

  340         0.50/0.47

  420        5,000/3,890


   15.5         50/53
   32         10.0/9.9
    8.4        7.0/7.0
   Date/Time
    Analyst
06-09/0930/TO
04-18/0830/KT

04-30/1500/JS

05-02/0815/KT


05-12/1400/RC
05-12/1300/DT
04-28/1400/JS
    dsl
                                       86-2736

-------
      •p
      'EST-PA1NE
     IojbotCLt&u&b INC. i
nn asm AVC. • BATON ROUGE. LA rono
                              IT CORPORATION
                           Port Allen,  Louisiana
                               June 18, 1986
    Sample Identification:   F1208.  0" - 6"
    Date Received:   04-15-86
    Parameter               Result
    Oil & Grease  (mg/kg)  12,900
    Total Organic Carbon
    (mg/kg C)
    Total Phosphate
    (mg/kg P)
    Total Nitrogen
    (mg/kg N)
    Cation Exchange
    Capacity  (meq/lOOg)
    Saturated Paste
    Extract:
    Chloride  (mg/kg Cl)
    Sulfate  (mg/kg SO.)
    Sodium Absorption
    Ratio
    pH (Units)
18,500
   360
   400
     8.2
    28.0
    32

     1.3
     8.6
           Quality Assurance
             Actual/Found
               10.0/11.6
  200/192

 0.50/0.47

5,000/3,890

0.250/0.250


   50/53
 10.0/9.9

0.250/0.250
  7.0/7.0
                    Date/Time
                     Analyst
                 04-28/1400/TO
04-18/0830/KT

04-30/1500/JS

05-02/0815/KT

     05-08/VM


05-12/1400/RC
05-12/1300/DT

     05-08/VM
04-28/1400/JS
                                                                 Otf OT-JC

-------
      'p
      EST-PAIN E
7979 OSMI AVt • BATON MUQE. LA 70UO
                              IT CORPORATION
                           Port Allen,  Louisiana
                               June 18,  1986
    Sample Identification:   F1209.  6" - 12"
    Date Received:   04-15-86
    Parameter               Result
    Oil  &  Grease (mg/kg)  10,700
    Total  Organic Carbon
     (mg/kg C)             15,200
    Total  Phosphate
     (mg/kg P)
    Total  Nitrogen
     (mg/kg N)
    Cation Exchange
    Capacity (meq/lOOg)
    Saturated Paste
    Extract:
    Chloride (mg/kg Cl)
    Sulfate (mg/kg S04)
    Sodium Absorption
    Ratio
    pH (Units)
280
440
  9.1
 22.5
 32

  1.4
  8.4
        Quality Assurance
          Actual/Found
            10.0/8.4
  200/192

 0.50/0.47

5,000/3;-890

0.250/0.250


   50/53
 10.0/9.9

0.250/0.250
  7.0/7.0

                    Date/Time
                     Analyst
                 06-09/0930/TO
04-18/0830/KT

04-30/1500/JS

05-02/0815/KT

     05-08/VM


05-12/1400/RC
05-12/1300/DT

     05-08/VM
04-28/1400/JS

   dsl
                                    86-2736

-------
      EST-PAINE
             GA we. i
nn asm AVI. • BATON NOUOE. LA mao
Parameter              	

Oil & Grease  (mg/kg)  1,560
                              IT CORPORATION
                           Port Allen,  Louisiana

                               June 18,  1986
    Sample Identification:   F1210.  12" - 18"

    Date Received:   04-15-86
                                     Quality Assurance
                                       Actual /Found

                                         10.0/8.4
                                      200/192


                                     0.50/0.47


                                    5,000/3,890





                                       50/53

                                     10.0/9.9

                                      7.0/7.0
    Total Organic  Carbon
    (mg/kg C)

    Total Phosphate
    (mg/kg P)

    Total Nitrogen
    (mg/kg N)

    Saturated Paste
    Extract:

    Chloride  (mg/kg  Cl)

    Sulfate  (mg/kg SO4)

    pH  (Units)
6,600
  300
  280
   18.0

   46

    8.6
                                  Date/Time
                                  Analyst

                              06-09/0930/TO
04-18/0830/KT


04-30/1500/JS


05-02/0815/KT





05-12/1400/RC

05-12/1300/DT

04-28/1400/JS

-------
       •p
        EST-PA1NE
       ^aJxn&totisA INC. i
 7979 QSfll AVE. • BATON AOUGC. LA 70(20
                               IT CORPORATION
                            Port Allen, Louisiana

                                June 18, 1986
      Sample Identification:  F1211. 18" - 24"

      Date Received:  04-15-86
      Parameter

      Oil & Grease (mg/kg)

      Total Organic Carbon
      (mg/kg C)

      Total Phosphate
      (mg/kg P)

      Total Nitrogen
      (mg/kg N)

      Saturated Paste
      Extract:

      Chloride (mg/kg Cl)

      Sulfate (mg/kg SO.)

      pH (Units)
          Quality Assurance
 Result     Actual/Found

1,070         10.0/8.4
5,700          200/192


  260         0.50/0.47



  500        5,000/3,890





   14.5         50/53

   29         10.0/9.9

    8.5        7.0/7.0
   Date/Time
    Analyst

06-09/0930/TO

        c

04-18/0830/KT



04-30/1500/JS



05-02/0815/KT





05-12/1400/RC

05-12/1300/DT

04-28/1400/JS
I
   dsl
                                                                 QC

-------
      •p
      EST-PAINE
nn QSRI AVI. • iATON Mouot. LA rouo
                              IT CORPORATION
                           Port Allen,  Louisiana
                               June 18,  1986
    Sample Identification:   F1212.  0"  - 6"
    Date Received:  04-15-86
    Parameter               Result
    Oil & Grease  (ing/kg)  21,900
    Total Organic Carbon
     (rag/kg C)
    Total Phosphate
     (mg/kg P)
    Total Nitrogen
     (mg/kg N)
    Cation Exchange
    Capacity  (meq/lOOg)
    Saturated Paste
    Extract:
    Chloride  (mg/kg  Cl)
    Sulfate  (mg/kg SO.)
    Sodium Absorption
    Ratio
    pH  (Units)
22,900
   260
   380
     8.2
    17.0
    33

     1.1
     8.4
           Quality Assurance
             Actual/Found
               10.0/8.4
  200/192

 0.50/0.47

5,000/3,890

0.250/0.250


   50/53
 10.0/9.9

0.250/0.250
  7.0/7.0
                    Date/Time
                     Analyst
                 06-09/0930/TO
04-18/0830/KT

04-30/1500/JS

05-02/0815/KT

     05-08/VM


05-12/1400/RC
05-12/1300/DT

     05-08/VM
04-28/1400/JS
                                                                 86-2736

-------
      EST-PAIN E
7979 OSNI AVI. • BATON NOUOC. LA 70(20
                              IT CORPORATION
                           Port Allen,  Louisiana
                               June 18,  1986
    Sample Identification:   F1213.  6" - 12"
    Date Received:   04-15-86
Parameter              Result
Oil & Grease  (mg/kg)  3,390
    Total Organic  Carbon
     (mg/kg  C)
    Total Phosphate
     (mg/kg  P)
    Total Nitrogen
     (mg/kg  N)
     Cation  Exchange
     Capacity (meq/lOOg)
     Saturated Paste
     Extract:
     Chloride (mg/kg Cl)
     Sulfate (mg/kg SO4)
     Sodium  Absorption
     Ratio
     pH (Units)
                      6,700
                         260
                         400
                           7.1
                          18.5
                          26

                           2.0
                           9.0
                                     Quality Assurance
                                       Actual/Found
                                         10.0/8.4
  200/192

 0.50/0.47

5,000/3,890

0.250/0.250


   50/53
 10.0/9.9

0.250/0.250
  7.0/7.0
                    Date/Time
                     Analyst
                 06-09/0930/TO
04-18/0830/KT

04-30/1500/JS

05-02/0815/KT

     05-08/VM


05-12/1400/RC
05-12/1300/DT

     05-08/VM
05-05/1400/DT
   dsl
                                                                86-2736

-------
      'P
      EST-PAINE
nn asm AVC. • BATON «ooot LA nix
                              IT CORPORATION
                           Port Allen,  Louisiana
                               June 18,  1986
    Sample Identification:   F1214.  12" - 18"
    Date Received:   04-15-86
    Parameter
    Oil  & Grease  (rag/kg)
    Total Organic Carbon
     (mg/kg  C)
    Total Phosphate
     (mg/kg  P)
    Total Nitrogen
     (mg/kg  N)
    Saturated  Paste
    Extract:
    Chloride  (mg/kg Cl)
    Sulfate (mg/kg SO.)
    pH (Units)
          Quality Assurance
 Result     Actual/Found
1,100         10.0/8.4
4,500          200/192

  280         0.50/0.47

  400        5,000/3,890


   15.5         50/53
   40         10.0/9.9
    8.4        7.0/7.0
   Date/Time
    Analyst
06-09/0930/TO
04-18/0830/KT

04-30/1500/JS

05-02/0815/KT


05-12/1400/RC
05-12/1300/DT
04-28/1400/JS

-------
      •p
      'EST-PAINE
     IcuboiQt&ii&A INC. i
nn asm AVI. • BATON AOUOE. LA rauo
                              IT CORPORATION
                           Port Allen, Louisiana
                               June 18, 1986
    Sample Identification:   F1215.  18** - 24"
    Date Received:   04-15-86
    Parameter
Result
    Oil & Grease  (mg/kgJ   1,180
    Total Organic Carbon
    (mg/kg C)              4,100
    Total Phosphate
    (mg/kg P)                320
    Total Nitrogen
    (mg/kg N)               <200
    Saturated Paste
    Extract:
    Chloride  (mg/kg  Cl)       19.0
    Sulfate  (mg/kg S04)       27
    pH  (Units)                 8.8
Quality Assurance
  Actual/Found
    10.0/8.4
              200/192

             0.50/0.47

            5,000/3,890


               50/53
             10.0/9.9
              7.0/7.0
   Date/Time
    Analyst
06-09/0930/TO
                    04-18/0830/KT

                    04-30/1500/JS

                    05-02/0815/KT


                    05-12/1400/RC
                    05-12/1300/DT
                    05-18/1700/DT
                                                                 86-2736

-------
                                                                                                                 SHEET NO
PROJECT NAME
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                                 CHAIN  OF CUSTODY RECORD


                                	     SAMPLER(S)  SIGNATUR
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-------
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                                                                                                   /*

-------
      'EST-PAINE
               INC. _

7979 GSMI AVE. • BATON ROUGE LA 70820
                              SAMPLE ANALYSES
                                                     NOV 1 9
                                     for
                              IT CORPORATION
                         8124 South  Choctaw Drive
                       Baton Rouge,  Louisiana  70815
                         ATTENTION:   Ms.  Sue Cange
                             November 14,  1985
                                                                  PS

-------
      EST-PA1NE
             eA INC. i
T978 OSRI AVE • BATON ROUGE. LA 70020
                             IT CORPORATION
                         Baton Rouge, Louisiana
                            November 14, 1985
         Samples  collected  by IT Corporation as documented   by   the
    enclosed  chain-of-custody  forms,  were received   at  West-Paine
    Laboratories, Incorporated on October 17, 1985.  The samples  were
    analyzed   accoarding  to  the  Environmental  Protection  Agency
    protocol as referenced below:
    A.   Standard   Methods   for  the   Examination   of   Water
         Wastewater. 15th Edition, 1980:
         Parameter                          Method
         Chemical Oxygen Demand             508A
         Total Organic Carbon               505
         Total Suspended Solids             209B
         pH                                 423
         The results are reported on the following  pages.
and
                                             lanager

-------
      EST-PAINE
               we.
7979 OSRI AVE. • BATON ROUGE. LA 70120
                             IT CORPORATION
                         Baton Rouge, Louisiana

                            November  14,  1985
    Sample Identification:  Control. Plot  1.  10-l7-85/1010hrs,

    Date Received:  October 17.  1985
    Parameter
    pH (Units)

    Total Organic Carbon
    (mg/L C)

    Total Suspended Solids
    (mg/L)

    Chemical Oxygen Demand
    (mg/L 02)
         Quality Assurance
Results    Actual/Found
  8.0


  2


 32.7



 13
7.0/7 . 0


 25/23


 50/49



100/109
                 Date/Time
                  Analyst
10-17/1650/NB


11-12/1200/BT


10-18/1400/KW



10-18/1050/NB

-------
      EST-PAIN E
7«79 GSRI AVE • BATON ROUOE. LA 70820
                             IT CORPORATION
                         Baton Rouge, Louisiana

                            November 14,  1985
    Sample Identification:  Group 1. Plot  2.  10-17-85/1045hrs,

    Date Received:  October 17. 1985
    Parameter
    pH (Units)

    Total Organic Carbon
    (mg/L C)

    Total Suspended Solids
    (mg/L)

    Chemical Oxygen Demand
    (mg/L 02)
         Quality Assurance
Results    Actual/Found
  8.0


  8


157


 27
7.0/7.0


 25/23


 50/49


100/109
                 Date/Time
                  Analyst
10-17/1650/NB



11-12/1200/BT



10-18/1400/KW



10-18/1050/NB

-------
nn asm AVE. • BATON AOUGE. LA TOUO
                             IT CORPORATION
                         Baton Rouge, Louisiana

                            November 14,  1985
    Sample Identification:  Group 1. Plot  3.  10-17-85/1100hrs,

    Date Received:  October 17. 1985
    Parameter
    pH (Units)

    Total Organic Carbon
    (mg/L C)

    Total Suspended Solids
    (mg/L)

    Chemical Oxygen Demand
    (mg/L 02)
         Quality Assurance
Results    Actual/Found
  8.1


 10


 13.7


 37
7.0/7.0


 25/23


 50/49


100/109
                 Date/Time
                  Analyst
10-17/1650/NB


11-12/1200/BT



10-18/1400/KW



10-18/1050/NB

-------
      EST-PAINE
7979 QSRI AVE • BATON RQUQE, LA 70(20
                             IT CORPORATION
                         Baton Rouge, Louisiana

                            November 14,  1985
    Sample Identification:  Group 2. Plot 4.  io-l7-85/1115hra.

    Date Received:  October 17. 1985
    Parameter
    pH (Units)

    Total Organic Carbon
    (mg/L C)

    Total Suspended Solids
    (mg/L)

    Chemical Oxygen Demand
    (mg/L 02)
         Quality Assurance
Results    Actual/Found
  8.1


 20


  4.2


 68
7.0/7.0


 25/23


 50/49


100/109
                 Date/Time
                  Analyst
10-17/1650/NB


11-12/1200/BT


10-18/1400/KW



10-18/1050/NB

-------
      p
      EST-PAINE
             66 we.
7979 GSBI AVE. • BATON ROUOE. LA 70WO
                              IT  CORPORATION
                         Baton Rouge,  Louisiana

                            November 14,  1985
    Sample Identification:  Group  2.  Plot 5.  10-17-85/113Ohrs.

    Date Received:  October 17.  1985
    Parameter
    pH (Units)

    Total Organic Carbon
    (mg/L C)

    Total Suspended Solids
    (mg/L)

    Chemical Oxygen Demand
    (mg/L 02)
         Quality Assurance
Results    Actual/Found
  8.1


 18


 12.2


 74
7.0/7.0


 25/23


 50/49


100/109
                 Date/Time
                  Analyst
10-17/1650/NB



11-12/1200/BT


10-18/1400/KW



10-18/1050/NB

-------
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-------
      p
      EST-PAINE
               INC.
7979 GSRI AVE • BATON ROUGE. LA 70820
                                                 rffltRNATIONAl
                             SAMPLE ANALYSES      TECHNOLOGY

                                                  JAN 06 1986
                                    for
                              IT  CORPORATION
                        8124  South  Choctaw Drive
                      Baton Rouge,  Louisiana  70815
                      ATTENTION:  Mr.  Jeffery Cange
                             December 30,  1985
                                                                 85-4800
       nal

-------
      EST-PAIN E
               INC.
7979 QSPl AVE • BATON ROUGE. LA 70820
                             IT CORPORATION
                         Baton Rouge, Louisiana
                            December 30, 1985
         Samples  collected  by IT Corporation as documented  by  the
    enclosed  chain-of-custody  forms,  were received  at  West-Paine
    Laboratories, Incorporated on November 1, 1985.  The samples were
    analyzed   accoarding  to  the  Environmental  Protection  Agency
    protocol as referenced below:
    A.
Standard
Methods
for  the   Examination  of  Water
and
         Wastewater. 15th Edition, 1980:
         Parameter
         Nitrate
         Nitrite
         Total Kjeldahl Nitrogen
         Total Dissolved Solids
         Total Organic Carbon
         Specific Conductance
                                   Method
                                   418C
                                   419
                                   417A, 417B
                                   209B
                                   505
                                   205
         The results are reported on the following pages.
                                             /
                                            Burton A.  Boeneke
                                            Environmental Coordinator
      nal
                                                        85-4800

-------
      EST-PAINE
               we.,
7979 GSRI AVE • BATON ROUGE. LA 70820
                              IT  CORPORATION
                         Baton Rouge,  Louisiana

                             December 30,  1985
    Sample ID:  Control Plot  #1.  Como.  of Container's #0145 & 01A6

    Date Received:  November  1.  1985
    Parameter
         Quality Assurance
Results    Actual/Found
                    Date/Time
                     Analyst
    Specific Conductance
    (umhos/cm)

    Total Dissolved
    Solids (mg/L)
680
330
  720/720
11-19/1500/ML
5,000/4,945     12-02/0930/TO
       nal
                                                                85-4800

-------
      EST-PAINE
7979 GSBI AVE • BATON ROUGE. LA 70820
                             IT CORPORATION
                         Baton Rouge,  Louisiana

                            December  30,  1985
    Sample ID:  Group 1. Plot  #2. Como.  of  Container's #0153 & 0154

    Date Received:  November 1.  1985
    Parameter
         Quality Assurance     Date/Time
Results    Actual/Found          Analyst
    Specific Conductance
    (umhos/cm)

    Total Dissolved
    Solids (mg/L)
700
338
  720/720
11-19/1500/ML
5,000/4,945    12-02/0930/TO
      nal
                                85-4800

-------
      EST-PAINE
               INC. <
7979 GSBI AVE • BATON ROUGE. LA 70420
                             IT CORPORATION
                         Baton Rouge, Louisiana

                            December  30,  1985
    Sample ID:   Group ],,	Plot #3. Como.  of  Container's #0161.
                 0163 and 0164
    Date Received:  November 1. 1985
    Parameter
         Quality Assurance
Results    Actual/Found
                   Date/Time
                    Analyst
    Specific Conductance
    (umhos/cm)

    Total Dissolved
    Solids (mg/L)
740
411
  720/720
11-19/1500/ML
5,000/4,945     12-02/0930/TO
      nal
                                85-4800

-------
      f>
      EST-PAINE
               INC.
7979 GSRI AVE • BATON ROUGE. LA 70620
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             December 30,  I9B5
    Sample ID:   Group  2.  Plot #4.  Comp.  of Container's #0169.  0170.
                 and 0171

    Date Received:  November 1.  1985
    Parameter
    Specific Conductance
    (umhos/cm)

    Total Dissolved
    Solids  (mg/L)
         Quality Assurance
Results    Actual/Found
                   Date/Time
                    Analyst
990
566
  720/720
11-19/1500/ML
5,000/4,945     12-02/0930/TO
        nal
                                                                 85-4800

-------
      EST-PAINE
               INC.,
    !><#•	
7979 GSRI AVE • BATON ROUGE. LA 70820
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             December 30, 1985
    Sample ID:   Group 2.  Plot  #5.  Como.  of Container's  #0177.  oi?fl

    Date Received:  November  1.  1985
                                         Quality Assurance      Date/Time
    Parameter                   Results    Actual/Found          Analyst
    Specific Conductance
    (umhOS/cm)                  765           720/720       11-19/1500/ML

    Total Dissolved
    Solids (mg/L)               332         5,000/4,945     12-02/0930/TO
      nal                                                         85-4800

-------
      EST-PAINE
               we.
7979 GSRI AVE • BATON ROUGE. LA 70820
                             IT CORPORATION
                         Baton Rouge, Louisiana

                            December 30, 1985
    Sample ID:    Group 2. Plot 15

    Date Received:   November 1. 1985
    Parameter
         Quality Assurance
Results    Actual/Found
                  Date/Time
                   Analyst
    Total  Organic
    Carbon (mg/L C)

    Total  Kjeldahl
    Nitrogen (mg/L N)

    Nitrate (mg/L N)

    Nitrite (mg/L N)
 10


 14.56

  2.6

 <0.05
  25/22


 100/95

0.50/0.50

0.50/0.50
12-04/1600/BT



12-09/1620/RH

12-02/0900/RC

12-02/0900/RC
     nal
                                 85-4800

-------
s
ui
•fl
o
VI
»
u

is

-------
       -L
PROJECT NAME
PROJECT NO
               0\c\
                                                      CHAIN  OF CUSTODY  RECORD

                                                                     SAMPLER(S)  SIGNATl
REllNOUISHtl 81  NAME
RELINQUISHED BY  NAME _.
RELINQUISHED BY  NAME.	
AUTHORISATION FOR DfSPOSAL
. DATE/TIME
. DATE/TIME
. DATE/TIME
 DAT E / TIME
                                                                    RECEIVED BY NAME .
                                                                    PECE1.ED BY NAME
                                                                                                                    DATE/TiME 	
                                                                                                                	DATE/TIME.

-------

-------
                                                          CHAIN  OF  CUSTODY RECORD

                                                                          SAMPLER(S) SIGNATURE
PROJECT  NAME
PROJECT  NO
                                                                          RECEIVED  BY
                                                                          RECEIVED  BY  NAME
                                                                          RECEIVED BY  NAME
                                                                          DISPOSED BY
RELINQUISHED BY  NAME
RELINQUISHED BY  NAME
RELINQUISHED BY  NAME
AUTHORIZATION FOR DISPOSAL

-------
                   CHAIN  OF  CUSTODY RECORD
PROJECT NAME
PROJECT NO.
C
                                   SAMPLERIS) SIGNATURE
RELINQUISHED BY
RELINQUISHED BY  NAME	
RELINQUISHED BY  NAME 	
AUTHORIZATION FOR DISPOSAL.
      DATE /TIME
      DATE/TIME
      DAT E / TIME
                                                                           BECtlvED BY NAME ;
                                                                           RECEIVED BY NAME .
                                                                           RECEIVED BY NAME
                                                                           Dl   OSED BY.  	
DATE/TIME
DATE/TIME
DATE/TIME
DATE/TIME

-------
       •p
       EST-PAINE
 797* OSm AVI. • BATON NOUQi. LA 70UO
                               SAMPLE ANALYSES
                                     for
                               IT CORPORATION
                          8124. South Choctaw Drive
                        Baton Rouge, Louisiana   70815
                          ATTENTION:  Ms. Sue  Cange
                              January 23,  1986
I
                                                                oc

-------
      EST-PAINE
    JcL6otettxni0A INC.
7*71 OSNI AVI. • BATON ROUGt LA 70820
                             IT CORPORATION
                         Baton Rouge, Louisiana
                            January 23,  1986
         Samples  collected  by IT Corporation as documented  by  the
    enclosed  chain-of-custody  forms,  were received at  West-Paine
    Laboratories,  Incorporated  on December 13,  1985.    The samples
    were  analyzed according to the Environmental   Protection  Agency
    protocol as referenced below:
    A.   Standard   Methods   for  the   Examination   of  Water .  and
         Wastewater. 15th Edition, 1980:
         Parameter
         Chemical Oxygen Demand
         Total Organic Carbon
         Total Suspended Solids
         PH
         Total Phosphorus
         Total Dissolved Solids
         Specific Conductance
         Total Kjeldahl Nitrogen
         Nitrate
Method
508A
505
209D
423
424C, 424F
209B
205
420A, 417A, 417D
418C
         The results are reported on the  following pages.
                                          Manager
                                                            rd,  III
                                                            oc noii

-------
      EST-PAINE
7979GSMIAVC. • BATON HOUGE. LA 7M20

                              IT CORPORATION
                         Baton Rouge,  Louisiana

                            January 23,  1986
 Sample Identification:  Control  Sump <1.  0222

 Date Received:  December  13.  1985
Parameter
Total Organic Carbon
(mg/L C)

Chemical Oxygen Demand
(mg/L 02)
                                       Quality Assurance     Date/Time
                             Results     Actual /Found         Analyst
 5


25
                                            25/25


                                           100/100
                                                         01-05/0600/JS-


                                                         12-19/0900/RC

-------
      •p
      EST-PAINE
               INC. i
7979 Htm AVI. • BATON (WOO!. LA 7M20
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             January 23,  1986


 Sample Identification:   Group #1.  Sump #1. 0224

 Date Received:  December 13.  1985
 Parameter
 Total Organic Carbon
 (ng/L C)

 Chemical Oxygen Demand
 (mg/L 02)
          Quality Assurance  /   Date/Time
Results     Actual/Found          Analyst
  9


 45
 25/25


100/100
01-05/0600/JS-


12-19/0900/RC


-------
     EST-PAINE
            eA we.,
      • BATON nOUOC. LA 7M20
                            IT CORPORATION
                        Baton Rouge, Louisiana

                           January 23, 1986
Sample Identification:  Group #1. Sump 13. 0226

Date Received:  December 13. 1985
                                     Quality Assurance     Date/Time
Parameter                  Results     Actual/Found          Analyst
Total Organic Carbon
(mg/L C)                    16             25/25        01-05/0600/JS-

Chemical Oxygen Demand
(mg/L 02)                   54             100/100       12-19/0900/RC


-------
      EST-PA1NE
              A INC.
7*7* asm AVI • BATON ftouat. LA ratio
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             January 23, 1986
 Sample Identification:   Group 12.  Sumo 14. 0228

 Date Received:   December 13.  1985
 Parameter
 Total Organic  Carbon
 (mg/L C)
          Quality Assurance      Date/Time
Results     Actual/Found          Analyst
480
 Chemical Oxygen Demand
 (mg/L 02)                 2,860
 25/25


100/100
01-05/0600/JS-



12-19/0900/RC

-------
     EST-PAINE
        aloti6A me.
      • BATON BOOOt L» 7M20
                            IT CORPORATION
                        Baton Rouge, Louisiana

                           January 23, 1986
Sample Identification:  Grouts 12. Sumo *5. 0230

Date Received:  December 13. 1985
                                     Quality Assurance     Date/Tine
Parameter                  Results     Actual/Found          Analyst
Total Organic Carbon                                           ,
(mg/L C)                    40             25/25        01-05/0600/JS-

Chemical Oxygen Demand
(mg/L 02)                  145             100/100       12-19/0900/RC


-------
      EST-PAINE
         atonteA we. -.

r»7» OSMI AVl • IATOM MOUOf. tA 70«0
                             IT CORPORATION
                         Baton Rouge, Louisiana

                            January 23,  1986
 Sample Identification:  Control Sumo  II.  0221

 Date Received:  December 13. 1985
 Parameter
 pH (Units)

 Total Suspended
 Solids (mg/L)
          Quality Assurance     Date/Time
Results     Actual/Found         Analyst
  7.1
  6.8
7.0/7.0
 50/48
12-13/1630/TO
12-18/1200/TO

-------
      EST-PA1NE
         atoti6A INC. •

nn asm AVI. • BATON nouot. LA ratio
                              IT CORPORATION
                          Baton Rouge, Louisiana

                             January 23, 1986
 Sample Identification:   Group II.  Sump #2. 0223

 Date Received!   December 13.  1985
 Parameter
 pH  (Units)

 Total Suspended
 Solids  (mg/L)
          Quality Assurance     Date/Time
Results     Actual/Found         Analyst
  6.8
 14.2
7.0/7.0



 50/48
12-13/163 0/TO


12-18/1200/TO


-------
nn asm AVI. • SATON nouot. LA mat
                              IT CORPORATION
                         Baton  Rouge,  Louisiana
                            January 23,  1986
 Sample Identification:  Group  <1.  Sump 13.  0225
 Date Received:  December  13. 1985
                                       Quality Assurance     Date/Time
 Parameter                  Results     Actual/Found         Analyst
 pH (Units)                    7.9          7.0/7.0      12-13/1630/TO
 Total Suspended
 Solids  (mg/L)                11.8           50/48       12-18/1200/TO

-------
       EST-PA1NE
              6A INC.
 7979 S»m AVi • BATON ROUQl LA 7M20
                               IT CORPORATION
                          Baton  Rouge,  Louisiana

                             January 23,  1986


  Sample Identification:  Group  *2.  Sumo  *4. 0227

  Date Received:  December  13. 1985
                                        Quality Assurance     Date/Time
  Parameter                   Results     Actual/Found         Analyst
  pH (Units)                    7.9          7.0/7.0      12-13/1630/TO

  Total Suspended
  Solids  (mg/L)                15.6           50/48       12-18/1200/TO
I

-------
      EST-PAINE
              A 1KC. i
797» QSMI AVI. • SATON HOUdf. LA 7QOO
                              IT CORPORATION
                          Baton Rouge, Louisiana

                             January 23, 1986
 Sample Identification:   Group »2. Sumo 15. 0229

 Date Received:   December 13.  1985
Parameter
pH (Units)
          Quality Assurance
Results     Actual/Found
                                                              Date/Tine
                                                               Analyst
  7.9
                                            7.0/7.0       12-13/1630/TO

-------
      EST-PAINE
              A INC. <
nn QSM Ave. • BATON aouat, LA rota
1
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             January 23,  1986
 Sample Identification:   Group *1.  Plot 12.  0233

 Date Received:  December 13.  1985
                                       Quality Assurance     Date/Time
 Parameter                   Results     Actual/Found         Analyst
 pH  (Units)                    7.8          7.0/7.0      12-13/1630/TO

 Total Organic
 Carbon  (mg/L C)              20             25/22       01-07/0800/RC

 Total Phosphate
 (mg/L P)                      0.06         1.0/1.0      01-02/1000/RG

-------
     EST-PAINE
        atatieA INC. •

      • BATON MOUQC. LA 70820
                            IT CORPORATION
                        Baton Rouge,  Louisiana

                           January  23,  1986
Sample Identification:  Group  II.  Plot  »3.  0235

Date Received:  December 13. 1985
Parameter
                                     Quality Assurance
                           Results     Actual/Found
                   Date/Time
                    Analyst
pH (Units)

Total Dissolved
Solids (mg/L)

Specific Conductivity
(umhos/cm)
                              7.8


                            569


                            840
  7.0/7.0      12-13/1630/TO



5,000/5,088    12-18/1000/TO



  720/720      01-13/1500/MS

-------
      EST-PAINE
               IMC. i
nn asm AVE. • §ATON aouat LA TOHO
                             IT CORPORATION
                         Baton Rouge, Louisiana
                            January 23, 1986
 Sample Identification:  Group »2. Plot  *5.  0239
 Date Received:  December 13. 1985
 Parameter
pH (Units)
Total Organic
Carbon (mg/L C)
Total Phosphate
(ng/L P)
Total Dissolved
Solids (ng/L)
Specific Conductivity
(umhos/cm)
Total Kjeldahl
Nitrogen  (mg/L N)
Nitrate (mg/L N)
Nitrite (mg/L N)
          Quality Assurance
Results     Actual/Found
                              7.7

                              7

                              0.06

                             628

                             850

                              2.9
                              <0.05
                              <0.05
               7 . 0/7 . 0
                25/22
               1.0/1.0
                                                            Date/Time
                                                             Analyst
12-13/1630/TO
01-07/08 00/RC
01-02/1000/RG
             5,000/5,088     12-18/1000/TO

               720/720       01-13/1500/MS
                100/99
               0.50/0.50
               0.50/0.50
12-26/1150/RG
12-31/1000/RG
12-31/1000/RG

-------
      'P
      EST-PAINE
         attn±&> we. —

nn asm *vt • BATON MOUQI. t* TOKO
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                             January 23,  1986
 Sample Identification:   Group 12.  Plot *4.  0247

 Date Received:  December 13,  1985
Parameter
pH (Units)

Total Dissolved
Solids (mg/L)

Specific Conductivity
(umhos/cm)
          Quality Assurance
Results     Actual/Found
                              7.9
               7.0/7.0
                                                             Date/Time
                                                              Analyst
                                                         12-13/1630/TO
705
                                          5,000/5,088    12-18/1000/TO
                               Insufficient Sample

-------
      EST-PAINE
             'jJA tur ,
7*79 QSMI AVf. • BATON MOUOf. LA 7IN30
                              IT CORPORATION
                         Baton Rouge,  Louisiana

                            January 23,  1986
 Sample Identification:  Group  12.  Plot *5.  0249

 Date Received:  December  13. 1985
 Parameter
 pH (Units)

 Total Organic
 Carbon (mg/L C)

 Total Dissolved
 Solids (mg/L)

 Specific C iductivity
 (umhos/cm)
          Quality Assurance     Date/Time
Results     Actual/Found         Analyst
  7.8


  8


670


820
  7.0/7.0
   25/22
12-13/1630/TO
01-07/0800/RC
5,000/5,088    12-18/1000/TO
  720/720
01-13/1500/MS

-------
      •p
      EST-PAINE
7*79 03*1 AVI • RATON HOUOi LA TOKO
                              IT CORPORATION
                         Baton Rouge,  Louisiana

                            January 22,  1986
 Sample Identification:  Group  tl.  Plot  *3.  0245

 Date Received:  December  13. 1985
 Parameter
 Total Organic
 Carbon  (mg/L C)

 Total Phosphate
 (ag/L P)

 Total Kjeldahl
 Nitrogen  (mg/L N)

 Nitrate (mg/L N)

 Nitrite (mg/L N)
          Quality Assurance
Results     Actual/Found
                  Date/Time
                   Analyst
 17


 <0.05


  7.1

 <0.05

 <0.05
  25/22


 1.0/1.0


 100/99

0.50/0.50

0.50/0.50
01-07/0800/RC-



01-02/1000/RG



12-26/1150/RH

12-31/1000/RG

12-31/1000/RG

-------
        EST-PAINE
                 INC.
 T»T» asm AVI • BATON MOUOE. LA TOKO
                               IT CORPORATION
                           Baton Rouge, Louisiana

                              January 23,  1986
   Sample Identification:  Group 12. Plot  »4.  0237

   Date  Received:   December 13. 1985
                                        Quality Assurance     Date/Time
  Parameter                  Results     Actual/Found         Analyst
  Total Organic
  Carbon  (ng/L C)              21              25/22        01-07/0800/RC.

  Total Phosphate
  (mg/L P)                     <0.05         1.0/1.0       01-02/1000/RG
I
                                                               •* s* /%«*«•«

-------
      EST-PAINE
               we.,
rtn asm AVE • «ATON nouat. L» rouo
                              IT  CORPORATION
                         Baton Rouge,  Louisiana

                            January 23,  1986
 Sample Identification:  Group *l.  Plot *2.  0243

 Date Received:  December  13.  1985
                                       Quality Assurance     Date/Time
 Parameter                   Results     Actual/Found         Analyst
 Total Dissolved
 Solids  (mg/L)C)           21721          5,000/5,088    12-18/1000/TO
                                                                      «
 Specific Conductivity
 (umhos/cm)                      Insufficient Sample
 nal                                                          86-0311

-------
        INTERNATIONAL
        TECHNOLOGY
        CORPORATION
PROJECT NAME/NUMBER
                                               CHAIN-OF-CUSTODY RECORD
                      LAB DESTINATION _1
                                                                                               R/A Control No.
                                                                                               C/C Control No.   Q006461
                                                                                                      /
                                  •^jr

SAMPLE TEAM MEMBERS TV \nt Wx\n ^i^L JT-H . !
                                                                CARRIER/WAYBILL NO.
    Sample
    Number
                        Sample
                  Location and Description
     Dale and Time
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                                                               Sample
                                                               Type
Container
 Type
Conditlon on Receipt
 (Name and Date)
Disposal
          Ccnrjrrl-Sornp .1
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Special Instructions:
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SIGNATURES:  (Name. Company, Date

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                                                              3. Relinquished By:

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                                                              4. Relinquished By:

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WHITE - To accompany tamplas
YELLOW -FI«W copy

-------
                                                                                                                     R/A Control No.
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PROJECT N
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O33I
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it.]i?/s5 ^2*^5
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YELLOW -FMd copy

-------
            "tKNATIUNAL
         .-CHNOLOGY
         CORPORATION
PROJECT NAME/NUMBER

SAMPLE TEAM MEMBERS
                                                                                                  R/A Control No.
                                \KNL-Ft7-.    fs5"~  5Of
CHAIN-OF-CUSTODY RECORD
                                                  C/C Control No.  Q00650

                  LAB DESTINATION   it 'K8* 11  riL\ fl g  ^L(3 b *3	
                                       O  V^
                                              CX*.
                                                                  CARRIER/WAYBILL NO.
    Sampla
    Number
                         Sample
                    Location and Description
                                                Date and Time
                                                  Collected
                 Sample
                  Type
Container
  Type
Condition on Receipt
 (Name and Date)
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Record No.
*«•'.

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Special Instructions:
Possible Sample Hazards:
SIGNATURES:  (Name. Company, Date and Time)

1. Relinquished I

  Received By:_^
                                                                  3.  Relinquished By:

                                                                     Received by:	
2. Relinquished By:

  Received By: 	
                                                                  4. Relinquished By:

                                                                    Received By:	
WHITE - To »ccomp«ny Mmptot
YELLOW -FMd copy

-------
      EST-PAINE
INTERNATIONAL
 TECHNOLOGY

 JlAR201QRn
7979~GSRI AVE. • BATON ROUQ8. LA 70820
                              SAMPLE ANALYSES
                                    for
                              IT CORPORATION
                         8124 South Choctaw Drive
                       Baton Rouge, Louisiana  70815
                         Attention:  Ms.  Sue Cange
                              March  14,  1986
                                                                  86-1200

-------
      EST-PAINE
7979 QSRI AVE. • BATON SOlXJt LA 70830
                              IT  CORPORATION
                          Baton Rouge,  Louisiana
                              March 14,  1986
         Samples  as designated  below,   and on the enclosed chain-of-
    custody documentation,  were received at West-Paine Laboratories,
    Incorporated on February  6,   1986.   The samples were analyzed for
    the   requested   parameters  according  to   the   Environmental
    Protection Agency protocol:
    A.   Standard   Methods   for  the   Examination  of  Water   and
         Wastewater. 15th Edition,  1980:
         Parameter
         pH
         Specific Conductance
         Total Dissolved Solids
         Total Suspended Solids
         Total Kjeldahl Nitrogen
         Chemical Oxygen Demand
         Total Organic Carbon
         Nitrate/Nitrite
         Total Phosphate
Method
423
205
209B
209D
420A, 417A, 417D
508
505
418C
424C, 424F
         The  results  are reported on the following pages.
                                                          nchard, III
                                             Manager
     dsl
                                                                 86-1200

-------
      EST-PA1NE
             te* INC.
7979 QSRI AVt • BATON TOUCH. LA 70820
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                              March 14,  1986
    Sample Identification:    SW.  Control Plot II. 1259 & 1260

    Date Received:   02-06-86
    Parameter

    pH  (Units)

    Total Suspended  Solids
    (mg/L)

    Total Organic  Carbon
    (mg/L C)

    Chemical Oxygen  Demand
    (mg/L 02)
         Quality Assurance     Date/Time
Results    Actual/Found         Analyst
 7.8
37
14
 7.0/7.0
50.0/48.8
               25/23
 100/94
02-06/1700/KT
02-06/2130/TO
              02-16/1400/RC
02-10/0830/KT
                                                                  oc 1

-------
      •p
      EST-PA1NE
7979 GSM AVC • BATON HOUOE. LA 70820
                              IT  CORPORATION
                         Baton Rouge,  Louisiana

                              March  14,  1986
    Sample Identification:   SW. Plot  2-5.  1261  &  1262

    Date Received:  02-06-86
    Parameter

    pH (Units)

    Total Suspended Solids
    (mg/L)

    Total Organic Carbon
    (mg/L C)

    Chemical Oxygen Demand
    (mg/L 02)
         Quality Assurance     Date/Time
Results    Actual/Found         Analyst
   7.9
 132
  29
 110
 7.0/7.0
50.0/48.8
  25/23
 100/101
02-06/1700/KT
02-06/2130/TO
02-16/1400/RC
02-12/0930/KT
     dsl

-------
7979 GSRI AVE. • BATON ROUQE. LA 70820
                              IT CORPORATION
                          Baton Rouge, Louisiana
                              March 14, 1986
    Sample  Identification:    Control Plot #1 1263 &  1275
    Date Received:   02-06-86
    Parameter
    pH  (Units)
    Specific Conductance
    (umhos/cm)
    Total Dissolved  Solids
    (mg/L)
    Total Organic  Carbon
    (mg/L C)
         Quality Assurance    Date/Time
Results    Actual/Found         Analyst
   8.0
 680
 937
  26
  7.0/7.0
  720/720
   25/23
02-06/1700/KT
02-25/1630/GS
5,000/4,918    02-06/2300/TO
02-16/1400/RC
     rlcl

-------
      EST-PAINE
7979 GSR! AVE. • BATON ROUGE. LA 70820
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                              March 14,  1986
    Sample Identification:   Group 1.  Plot #2 1264 & 1274

    Date Received:   02-06-86
    Parameter                 Results

    pH (Units)                   7.7

    Specific Conductance
    (umhos/cm)               1,000

    Total Dissolved Solids
    (mg/L)                   1,030

    Total Kjeldahl Nitrogen
    (mg/L N)                     3.5

    Nitrate (mg/L N)             0.05

    Nitrite (mg/L N)            <0.05

    Total Organic Carbon
    (mg/L C)                    20

    Total Phosphate
    (mg/L P)                    <0.1
Quality Assurance     Date/Time
  Actual/Found        Analyst
     7.0/7.0
    720/720
     100/102

    0.50/0.50

    0.50/0.50


      25/23


    1.00/1.04
 02-06/1700/KT
02-25/16-30/GS
   5,000/4,918    02-06/2300/TO
 02-14/1200/RH

 02-18/0800/DH

 02-18/0800/DH


 02-16/1400/RC



 02-13/1400/RH
      dsl
                        86-1200

-------
      EST-PAINE
     ICfjbo
7979 asm AVE. • BATON nouac. LA TOUO
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                              March 14, 1986
    Sample Identification:   Group 1.  Plot #3 1265 & 1272

    Date Received:   02-06-86
    Parameter                 Results

    pH (Units)                   7.3

    Specific Conductance
    (umhos/cm)               1,000

    Total Dissolved Solids
    (mg/L)                     745

    Total Kjeldahl Nitrogen
    (mg/L N)                     2.03

    Nitrate (mg/L N)             0.35

    Nitrite (mg/L N)            <0.05

    Total Organic Carbon
    (mg/L C)                    10

    Total Phosphate
    (mg/L P)                    <0.1
Quality Assurance     Date/Time
  Actual/Found        Analyst
     7.0/7.0


    720/720


   5,000/4,918


     100/102

    0.50/0.50

    0.50/0.50


      25/23


    1.00/1.04
 02-06/1700/KT



02-25/1630/GS



 02-06/2300/TO



 02-14/1200/RH

 02-18/0800/DH

 02-18/0800/DH



 02-16/1400/RC



 02-13/1400/RH
    dsl
                                                                  86-1200

-------
      EST-PAINE
7979 GSRI AVt • BATON POUQt LA 70820
                              IT CORPORATION
                          Baton Rouge,  Louisiana

                              March 14,  1986
    Sample Identification:   Group 2.  Plot #4 1266. 1270. & 1271

    Date Received:   02-06-86
    Parameter                 Results

    pH (Units)                   7.0

    Specific Conductance
    (umhos/cm)               1,100

    Total Dissolved Solids
    (mg/L)                     792

    Total Kjeldahl Nitrogen
    (mg/L N)                     2.1

    Nitrate (mg/L N)             2.9

    Nitrite (mg/L N)            <0.05

    Total Organic Carbon
    (mg/L C)                     5

    Total Phosphate
    (mg/L P)                    <0.1
Quality Assurance     Date/Time
  Actual/Found        Analyst
     7.0/7.0


  1,400/1,400


   5,000/4,918


     100/102

    0.50/0.50

    0.50/0.50


      25/23


    1.00/1.04
 02-06/1700/KT



02-25/1630/GS


 02-06/2300/TO



 02-14/1200/RH

 02-18/0800/DH

 02-18/0800/DH


 02-16/1400/RC


 02-13/1400/RH
      dsl
                                                                   86-1200

-------
      EST-PA1NE
     IaJtotototi
7979 GSfll AV6. • BATON ROUGE. l> 70MO
                              IT CORPORATION
                          Baton Rouge, Louisiana

                              March 14, 1986
    Sample  Identification:   Group 2. Plot #5 1267. 1268,  &  1269

    Date  Received:   02-06-86
    Parameter                Results

    pH  (Units)                   7.2

    Specific Conductance
     (umhos/cm)               1,000

    Total  Dissolved Solids
     (mg/L)                     694

    Total  Kjeldahl Nitrogen
     (mg/L  N)                     1.26

    Nitrate (mg/L N)             1.2

    Nitrite (mg/L N)            <0.05

    Total  Organic Carbon
     (mg/L  C)                    10

    Total  Phosphate
     (mg/L  P)                    <0.1
Quality Assurance    Date/Time
  Actual/Found
     7.0/7.0
02-06/1700/KT
  1,400/1,400    02-25/1630/GS
   5,000/4,918    02-06/2300/TO
     100/102

    0.50/0.50

    0.50/0.50


      25/23


    1.00/1.04
02-14/1200/RH

02-18/0800/DH

02-18/0800/DH



02-16/1400/RC



02-13/1400/RH
      rkl

-------
                                   \-\
                                                      CHAIN  OF CUSTODY  RECORD
PROJECT  NAME
PROJECT  MO
                                                                     SAMPLER(S)  SIGNATUR
RELINQUISHED BY: NAME
RELINQUISHED BY  NAME 	
JK'THORIZATION FOR DISPOSAL.
DATE/TIME
DATE/TIME
DATE /TIME
        BY
RECEIVED BY: NAME .
RECEIVED BY: NAME .
DISPOSED BY: 	
                                                                                                                     DATE /TIME ±jjt f **>   /VJ"
                                                                                                                     DATE/TIME
                                                                                                                     DATE/TIME .
                                                                                                                     DATE /TIME.
                                                                      -  6^lc\55  6 1
                                                                           J>v>

-------
                                                                                                                              SHEET NO
PROJECT NAME

PROJECT NO.
                                    CHAIN OF CUSTODY  RECORD


                                   	     SAMPLER(S) SIGNATUR
     SAMPLE
  IDENTIFICATION
SAMPLING
LOCATION
 DATE
SAMPLED
                                               SAMPLE TYPE
                                                   ID AIM  OIL
  VOLUME
  TO BE
COLLECTED
  NO. OF
CONTAINERS
   TIME
COLLECTION
   BEGAN
   TIME
COLLECTION
COMPLETED
                                                                                                                              COMMENTS
  .12.7:5
                                                             L
                                                                                                  Ar
                                                                                                                                       !.  }..
RELINQUISHED BY

RELINQUISHED BY-  NAME 	

RELINQUISHED BY  NAME 	

AUTHORIZATION FOR DISPOSAL.
                        t/ATE /TIME

                        DATE/TIME

                        DATE/TIME

                        DATE/TIME
                   //&/$&
     RECEIVED BY MAME .

     RECEIVED BY: NAME .

     RECEIVED BY: NAME .

     DISPOSED BY: 	
                                                                                       DATE/Tit
                                                                                                                           DATF/TIMF

-------
.KiTCRNATtONAL
                           OLD  IN6ER FIELD PLOT STUDIES
                          MICROTOX  SOIL CORE WSF RESULTS
1 £V^r11'**
^njrArJj
"k re.
. n0 iQflfi — -T- —
DEf TN1 U °
SAMPLE
EC50
\\fCfji- \ I /
iv- '-'-*•• 1 j c
LL5
7 -p\ fJU
I C**
SAMPLE
'#
"^r
EC50


LL5


SAMPLE


EC50


LL5

0-6"
6-12"
12-18"
18-24"


0-6"
6-12"
12-18"
18-24"


0-6"
6-12"
12-18"
18-24"


0-6"
6-12"
12-18"
18-24"


0-6"
6-12"
12-18"
18-24"
0306
0307
0308
0309


0310
0311
0312
0313


0314
0315
0316
0317


0318
0319
0320
0321


0322
0323
0324
0325
OTE
NTE
NTE
NTE


NTE
NTE
NTE
S


OTE
S
NTE
NTE


OTE
S
S
NTE


NTE
S
S
NT
22 0266
-3
17
15
— -RRflllP

-1
-3
3
-7
.GROUP

28
-19
3
8
rpniip

28
-13
-8
5
....GROUP

10
-19
-13
NT
0267
0268
0269
1, PLOT
0270
0271
0272
0273
1, PLOT
0274
0275
0276
0277
2, PLOT
0278
0279
0280
0281
2, PLOT
0282
0283
0284
0285
NTE
NTE
NTE
OTE
7 ....

99
OTE
NTE
NTE
1 ....

97
OTE
NTE
NTE
A ....

OTE
NTE
NTE
NTE

5....
70
OTE
NTE
NTE
19
18
15
24


33
35
3
17


39
22
19
4


27
14
10
10


50
28
18
7
3121
3122
3123
3124


3125
3126
3127
3128


3129
3130
3131
3132


3133
3134
3135
3136


3137
3138
3139
3140
OTE
OTE
OTE
NTE


75
OTE
76
NTE


62
OTE
83
NTE


OTE
OTE
OTE
NTE


90
OTE
OTE
NTE
30
21
22
15


47
33
36
11


55
35
46
7


37
31
33
3


47
37
28
8
EC50  Vol  % of the distilled,  de ionized  water  (DW)  extract  effecting  a  50% decrease
      1n b1olum1nescence during a 5  minute  test  period.   The  DW extract 1s obtainec
      by shaking 400  ml  DW + 100  g sample for  24 hours.

 LL5  The 5 minute mean  X light loss 1n  the combined  full strength  and  50% serial
      dilutions of the DW extract.

 NTE  No observed Toxic  Effect [reported when  the LL5 1s  less than  20X;  1f the LL5
      1s less than -5X,  stimulation  (S)  of  blolumlnescence  1s reported].

 OTE  Observed Toxic  Effect [reported when  a definite loss  of light 1s  observed
      (LL5>20X); however, the  level  of decrease  1n blolumlnescence  1s such that
      a quantitative  EC50 cannot  be  established].
  NT  Sample not tested.

-------
                                                                                                                             SHEET NO
PROJECT  NAME
CHAIN OF CUSTODY  RECORD
                SAMPLER (S)  SIGNATURE
RELINQUISHED BY
RELINQUISHED BY:  NAME 	
RELINQUISHED BY  NAME	
AUTHORIZATION FOR DISPOSAL.
                                           . DATE/TIME
                                            DATE/TIME
                                           . DATE/TIME
                                            DATE/TIME
  XCIVED BY NAME.
RECEIVED BY: NAME .
RECEIVED BY: NAME.
DISPOSED BY= 	
                                                                 DATE/TIME
                                                                 DATE/TIME -
                                                                 DATE/TIME .
                                                                 DATE/TIME.

-------
                                                                                                                             SHEET NO
PROJECT  NAME
PROJECT NGk
                                                         CHAIN  OF CUSTODY RECORD
                                                                        SAMPLER(S) SIGNATURE
RELINQUISHED BY:
RELINQUISHED BY:
RELINQUISHED BY
AUTHORIZATION FOR DISPOSAL .
NAME
.DATE/TIME <
. DATE/TIME
. DATE/TIME
. DATE/TIME .
  :CEIVED BY NAME .
RECEIVED BY: NAME .
RECEIVED BY: NAME
DISPOSED BY: 	
                                                                                                                           OATf/TIMF
DATE/TIME.
DATE/TIME.

-------
            6TAT1CM
                                                               CO ATE
3030
3037
J033
JA34
3039
3037
303H
30«A
jA4l
3042
3043
3044
3049
J046
30*7
3048
J049
3ASO
3051
3053
30S4
30*9
305*
3057
30S9
30bA
3Ao2
30o3
30e4
3Ao9
30e*
30oB
30o9
JA70
3071
4072
3073
3074
3A75
3A76
3077
3074
30*0
30«1
30»3
3A»4
30*5
30»6
30*3
3094
3099
JlgO
3101
31u7
31w3
       lei
      11»7
      lib*
      115*
      1909
      1910
      1914
      191S
      1917
      1918
      19-^0
      1922
      19*3
      1929
      192*
      19*7
      1928
      193ft
      1931
      194?
      1934
1937
193"
1939
1940
1941
1942
1943
1944
1949
      19*7
      19,«
      3037
J040
3041
3042
30*7  JA44
                  1 H.uT 7  0-«"   0101
                         3   u-o-   olo4
                         4  0-*«   010?
                      it 9  A-6'
                 2 0-*"
                 3 0-6"
            PLOT 4 ft.*-   »1*10
                 9 0-*"
                         0-^"  |0^0b
            G»OI'f I ?1;oT 2  0-
                                §020*
            0?«6
            02e*
      0770
      0771
      0772
      0273
      0274_
      u279
      0776
      0277
      027«
                         INTERNATIONAL
                          TECHNOLOGY

                           JUM06198G
            02*7
            0^12
            C313
            0'14
            0*16
            0317
            0319
            03*o
      03k?
      03.4
                   i    mor
      CPOUP i,  PLOT *   §1302
      CPuUP l.ftCT 3    tl3o1

               PbOT b
                    ••f.CiT I   «U73
            CONTHOt, «•! 0T 1   41374
            CPullr 1.
                  1,
                       H32*
                       tl*27
               PI.CT 3
      (,»uUP \, PbCT 3
            1. PLCt *  tl331
            i., PUCT 'i  «l3i2
                    4
            «,»C-"t» <
                          »  §1337
                                                                09/2U/»9
                                                                10/0&/b5
                                                                in/03/k5
                                                                10/10/89
                                                                10/10/09
                                                                09/20/**
                                                         09/70/b9
                                                         09/20/«9
                                                         11/29/U9
                                                         11/7S/H5
                                                         11/79/H9
                                                         11/25/49
                                                         11/25/89
                                                         01/2'4/bA
                                                         01/22/86
                                                         01/22/H6
                                                         OI/22/K6
                                                         01/22/86
                                                         01/22/S6
                                                         01/22/66"
                                                         01/22/86
                                                         01/22/86
                                                         01/22/86
                                                         01/22/86
                                                         01/22/86
                                                         01/22/86
                                                         01/22/86
                                                         01/22/86
                                                         01/22/86
                                                         01/22/B6
                                                         01/22/86
                                                         01/22/86
                                                         01/22/86
                                                         01/22/06
                                                         01/22/K6
                                                         01/22/06
                                                         01/22/86
                                                         01/22/86
                                                         01/2^/bfi
                                                         01/22/M6
                                                         01/22/H6
                                                         01/22/86
                                                         01/22/86
                                                         01/22/86
                                                         01/2^/66
                                                         01/22/86
                                                                01/22/U6
                                                                01/22/8*
                                                                01/72/86
                                                                01/22/8*
                                                                01/2-^/86
                                                                01/22/M6
                                                                U3/10/86
                                                                03/10/MA
                                                                03/10/86
                                                                03/10/86
                                                                03/10/M6
                                                                04/15/»6
                                                                04/lb/H6
                                                                04/15/H6
                                                                04/15/M6
                                                                04/tb/h6
                                                                04/15/B6
                                                                04/lb/d«
                                                                04/15/4i6
                                                                04/15/B6
                                                                U4/13/B6
                                                                04/ls/e6
                                                                04/15/«6
                                                                04/l«/«6
                                                                04/la/k*
                                                                04/lb/»6
                                                                04/ls/b*

-------
 PROJECT:  mo INGERS SAMPLES COLLECTED 4/is/w
          W.13
                                                        PRECISION REPORT
                                                     OUFUCATE ANALYSIS DATA
                                                         ABURPT.LSTJ671
 CONCENTRATION IN N6/K6 DRY UT.
SAHP
NO
TIME
DATE
ELMT
NA
K
CA
N6
FE
m
CO
NO
AL
AS
SE
CD
BE
01
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI


3036 * ANALYSIS DATA
 NO * LESS THAN LOD
ZRD * PERCENT RELATIVE DIFFERENCE
                                                                                                 EPA/RSKERL/ADA.OK

-------
                                                         PRECISION REPORT
                                                      DUPLICATE ANALYSIS DATA
                                                          ADURPT.LST?666
 PROJECT:   OLD INGERS SITE SANPLES COLLECTED 3/10/86
 CONCENTRATION IN N6/K6 DRY «T.
SAMP
NO
TINE
DATE
ELHT
NA
K
CA
H6
FE
HN
CO
NO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
AG
TL
PB
LI
SR
V
BA
B
TI
2483M)
18113
22HAY86
AVERAGE
111.
1240.
4S10.
2370.
16900.
562.
7.35
NO
15300.
ND
ND
ND
0.907
7.2?
18.9
11.4
34,7
ND
NO
13.5
8.61
23.4
33.7
218.
5.53
325.
ZRD
1.76
3.38
2.06
0.94
0.17
1.41
3.03

2.43



7.09
2.28
1.18
2.72
1.38


9.36
3.4
1.54
3.35
0.56
1.81
0.84
2487(A)
17:28
22HAY86
AVERAGE
150.
1650.
5290.
2700.
15800.
467.
6.9
5.43
17700.
ND
ND
ND
0.93
9.8S
24.5
14.
43.3
ND
ND
17.9
11.6
28.9
36.5
250.
6.48
368.
ZRD
7.4
1,39
•3.84
0.42
-3.76
-30.74
-29.15
-3.16
0.22



-0.97
-1,46
-3,8
-8,61
4.19


-19.21
2.06
4.84
-5.09
-5.9
-21.62
0.42
                                                       AVERA6E     ZRD
AVERAGE     ZRD
AVERAGE     ZRD
 * NEASURENENT DATA
(A) * ANALYSIS DATA
 ND * LESS THAN LOO
ZRD = PERCENT RELATIVE DIFFERENCE
                                                                                                   EPA/RSKERL/ADAiOK

-------
                                                         PRECISION REPORT
                                                      DUPLICATE ANALYSIS DATA
                                                          ADURPT.LSTJ663
 PROJECT:  OLD INGERS SITE SAMPLES COLLECTED 1/22/86
           P9.9
 CONCENTRATION IN N6/K6 DRY VT.
SAflP
NO
TINE
DATE
ELHT
NA
K
CA
H6
FE
HN
CO
HO
AL
AS
SE
CD
BE
CU
SB
CR
NI
ZN
A6
TL
PB
HG
LI
TE
SR
6E
V
DA
B
TI


1929
-------
                                                        PRECISION REPORT
                                                     DUPLICATE ANALYSIS DATA
                                                         ADURPT.LSTJ661
 PROJECT:   OLD INGERS SHE SAMPLES
           COLLECTED  1/22/86
           P9.1
 CONCENTRATION IN H6/K6
SANP
NO
TIKE
DATE
ELNT
MA
K
CA
MS
FE
m
CO
HO
AL
AS
SE
CD
BE
CU
SB
CR
NI
ZN
AG
TL
PB
HG
LI
TE
SR
6E
V
BA
B
TI


1909(N)
10M7
7NAY86
AVERAGE
149.
1550.
4950.
2690.
14800.
257.
4.96
KB
17100.
ND
14.2
KD
0.85
7,74
ND
22.2
13,4
37.2
NO
ND
14,1
ND
8.77
NO
24.6
ND
31.8
164.
6.65
338.



1909CA)
10
:o3
7NAY86
ZRD
6.28
6.17
0.76
3.54
3.38
2.43
1.22

2.91

5.91

3.74
6.38

1.81
1.04
1,47


18.94

9.97

3.52

4.59
3,2
8.14
0.24
AVERAGE
ISO.
1550.
4820.
2740.
15300.
288.
5.33
ND
17200.
ND
13.4
ND
0.878
7.92
ND
22,1
13.3
37.
NO
ND
13.5
ND
9.11
ND
25.
MB
32.4
174.
6.44
322.
ZRD
4.32
5.78
6.22
-0.34
-3.64
-19.47
-12.75

1.57

6.31

-2.79
1.56

2.69
2.47
2.54


-11.18

2.11

0.86

0.68
-6.64
14.71
10.42


1917(A)
10:24
7HAY86
AVERAGE
161.
1390.
4420.
2590.
14500.
246.
4.84
ND
16700.
KD
14.3
ND
0.826
6.77
ND
21.8
12.3
35.5
ND
ND
14.9
ND
7.97
ND
23.9
ND
30.4
160.
7.34
316.
ZRD
-6.2
-11.67
3.76
-3.3
-1.67
11.55
2.01

-4.06

-13.7

-11.75
-7.14

-2.06
-2.51
-1.27


-1,54

-6.71

-1.95

-2.43
-4.07
1.84
-6.21


1928(A)
10
M6
7HAY86
AVERAGE
144.
1270.
6450.
2300.
13000.
235.
4.62
NO
15600.
MB
12.7
ND
0.738
6.08
ND
20.9
11.6
33.9
ND
ND
16.1
NO
7,2
NO
34.5
NO
28.2
151.
4.18
292.
ZRD
-11.76
13.51
-57.6
2.06
2.32
3.91
5.64

3.59

7.81

8.86
1,09

6.53
4.65
0.7


5.96

1.4

-61.85

5.62
2.32
-40.93
21.7
                                                                                                    AVERAGE     ZRD
(H) > NEASURENENT DATA
(A) * ANALYSIS DATA
 NO * LESS THAN L09
ZRD * PERCENT RELATIVE DIFFERENCE
                                                                                                  EPA/RSKERL/ADA>OK

-------
PROJECT:  on INGERS SITE T.A.
          P1B4.8

CONCEKTRAnON IM N6/K6 VET UT.
                                                       PRECISION REPORT
                                                    DUPLICATE ANALYSIS MTA
                                                        ADURPT.LSTJ651
SAMP
NO
626 (H)
1155
2006 (A)
1155
336 (A) 4366(A)
11S9DUP GRIND 1159 DUP 016
OFDUP6RIMB
TINE
DATE
ELNT
NA
K
CA
M6
FE
W
CO
HO
AL
AS
SE
CD
BE
CU
CR
HI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
n:o?

300EC8S
AVERAGE
73.6
1260.
5010.
2420.
13000.
301.
5.04
HD
14700.
ND
ND
NO
0.703
6.51
16.?
11.2
39.3
NO
ND
10.2
8.3
22.4
28.8
152.
ND
270.
ZRD
7.74
3.41
1.17
0.47
0.5
0.45
5.02

2.16



1.39
1.99
2.02
1.25
1.02


11.33
4.7
0.66
0.85
1.3?

1.63
13
:56
30DEC85
AVER/WE
72.5
1270.
5050.
2420.
13000.
354.
5.46
KD
14800.
ND
ND
ND
0.712
6.61
16.8
11.1
36.3
ND
ND
9.89
8.45
22.8
29.1
158.
ND
272.
ZRO
10.85
2.64
-2.85
0.8
-1.71
-30.64
-10.74

0.98



-1.3
-1.1
3.7
0.41
14.95


-5.94
1.05
-3.29
-2.58
-6.89

-2.83
14:
04
30BEC85
AVERAGE
57.4
961.
5280.
2110.
12500.
29?.
5.55
1.49
12400.
NO
NO
ND
0.629
6.62
16.2
10.5
30.7
ND
ND
13.
6.95
23.9
26.2
163.
ND
210.
ZRD
7.83
-14.87
49.55
0.79
-7.06
-2.43
6.85
-33.75
-10.



-12.4
1.12
-5.79
-3.72
-0.33


-11.17
-0,94
23.
-14,82
-8.64

-12.62
14
:os
30DEC8S
AVERAGE
57.4
1080.
4030.
2120.
13000.
292.
5.17
1.67
13300.
ND
NO
ND
0.679
6.65
17,4
10.7
31.2
ND
ND
13.9
7.34
21.8
28.6
168.
ND
236.
ZRD
-8.08
-9.26
-2.84
-1.76
-1.18
7.51
7.11
8.66
-5.04



-3.22
-2.08
-7.36
-1.01
-2.88


-2.84
-9.73
-6.04
-3.07
1.61

-11.22
                                                                                                  AVERAGE   .  BtD
(H) * MEASUREMENT MTA
(A) * ANALYSIS DATA
 ND » LESS THAN LOB
ffO * PERCENT RELATIVE DIFFERENCE
                                                                                                 EPA/RSKERL/ABAtlX

-------
                                                         PRECISION REPORT
                                                      DUPLICATE ANALYSIS DATA
                                                          ADURPT.LSTJ637
 PROJECT:  INGERS SITE SAMPLES COLLECTED 9/20/93
           P181.8

 CONCENTRATION IN N6/KB UET HT.
SAMP
NO


TIME
BATE
ELHT
NA
K
CA
M6
FE
MM
CO
NO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
AG
TL
PB
LI
SR
V
BA
B
TI


180 (H)
PLOT

14212
1NOV8S
AVERAGE
103.
1130.
4430.
2310.
13400.-
441.
5.61
ND
14000.
NO
ND
ND
0.761
7.13
18.1
12.1
33.3
ND
ND
10.5
7.91
23.1
31.3
170.
3.27
278.
1 CONTROL
»


ZRO
9.93
10.28
1,73
0.32
0.12
1.52
0.9

1.58



6.4
1.24
0.96
0.26
1.1


1.11
6.61
0.21
2.32
0.77
7.42
2.05


180 (A)
0-6§ PLOT

13!45
1NOV85
AVERAGE
105.
1120.
4090.
2260.
13500.
382.
5.03
NO
13900.
ND
ND
ND
0.747
6.9
17.6
12.
32.8
ND
ND
1 CONTROL



184

(A)
0-6' GROUP 2 PLOT 2
0-6
14i
•
02
1NOV85
ZRD
6.59
12.35
14.8
5.04
-0.22
28.82
24.35

2.9?



10.16
7.96
7.13
1.5
1.87


11.3 -15.89
7.87
21.7
30.2
164,
7.74
12.69
5.31
8.32
5.08. -66.27
271.
3.68
AVERA6E
97.1
966.
3710.
1950.
11500.
221,
4.04
5.6
12100.
NO
ND
ND
0.623
7.51
18.6
10.4
35.5
ND
ND
15,3
7.1
21.8
25.8
186.
3.53
205.
ZRO
6.81
14.21
-17.65
3.47
2.23
-36.1
-9.21
-7.56
8.63



2.73
-0.09
5.08
-6.96
-15.8


-9.1
10,11
-13.27
5,34
-6.53
55.15
27,54

188
PLOT


(A)
0-6'

14:09
1NOV85
AVERAGE
94.7
967.
3610.
2050.
12200.
330.
4.42
ND
13400.
ND
ND
ND
0.67
6.43
16.6
10.1
30,2
ND
ND
10.
7.26
20.
27.5
141.
2.27
237.
ZRD
1.38
1.26
13.84
-0.07
3.16
84.82
38.47

3.99



1.82
0.74
4.57
3.66
1.33


27.81
5.06
15.56
-0.3
8.33
-63.25
1.33
                                                                                                     AVERAGE    ZRD
(H) * MEASUREMENT OATA
(A) * ANALYSIS DATA •
 ND * LESS THAN LOT
ZKB * PERCENT RELATIVE DIFFERENCE
                                                                                                  EPA/RSKERL/ADAtOK

-------
                                                       PRECISION REPORT
                                                    DUPLICATE ANALYSIS DATA
                                                        AOURPT.LSTU23
PROJECT:  INBERSAQC
          DIGESTED EXTRACTS FROM CONTRACT LAJ
CONCENTRATION IN H6/K6 VET VT,
SAW-
NO
TINE
DATE
ELNT
NA
K
CA
H6
FE
HN
CO
NO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
AG
TL
PB
LI
SR
V
BA
B
TI



1247 -- HEASUREMENT DATA
(A) - ANALYSIS DATA
 ND = LESS THAN LOB
ZRD ' PERCENT RELATIVE  DIFFERENCE
                                                                                                 EPA/RSKERL/ABAtOK

-------
                                                ELEMENTAL CONSTITUENTS ANALYSIS  BY  ICAP
                                                          FOR:
                                                            LIST.LSTI2247
 PROJECT:  OLD INGERS SAMPLES COLLECTED 4/15/86
           P9.13

 CONCENTRATION INI   M6/K6 DRY UT.

DATE        22MAY86      STDV           22MAY86
TIME        18108         +/-           1811
TA6.NO.      3054          3054          3054BUP

ELEMENT      VALUE         VALUE        VALUE
22MAYB6
18H1
  3055

 VALUE
STDV
 f/-
   3055

  VALUE
VAUC
VALUE
NA
K
CA
M6
FE
HN
CO
HO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
AC
TL
PB
LI
SR
V
BA
B
TI
124,
1410.
4130.
2310.
15600.
311.
5.03
<1.
18000.
<22.
<12.
<.2
0.91
8.1
20.4
11.5
35.0
<.9
<5.
13.7
9.9
23.1
38.0
175.
4.6
349.
13.
140.
410.
•230.
1500.
30.
0,97
1.5
1800.
22.
12.
0.28
0.30
2.4
2.0
1.2
3.8
0.97
5.4
5.7
1.0
2.3
4.6
17.
1.5
34.
108.
989.
3990.
2190.
15200.
279.
4.4
<1.
15700.
<19.
<11.
<.2
0,79
7.8
19.5
11.8
36.2
<1.
<4.
15,2
8.30
20.8
33.2
170.
3.7
260.
11.
99.
400.
220.
1500.
27.
1.0
1.3
1500.
19.
11,
0.29
0.30
2.4
1.9
1.2
3.9
1.0
4.8
5.3
0.98
2.0
4.1
17.
1.3
26.
107.
1140.
4000.
2290.
15000.
271.
5.34
<1.
16100.
<19.
<11.
<,2
0,82
7.6
19.4
11.6
35.0
<.9
<4.
15.9
8.60
21.5
35,8
166.
5.1
298.
11.
110.
400.
230.
1500.
26.
0.95
1.4
1600.
19.
11.
0.28
0.29
2.3
1.9
1.2
3.8
0.96
4.9
5.4
0.94
2.1
4.3
16.
1.3
29.
< VALUE«LINIT OF DETECTION DETERMINE! BY INSTRUMENT SENSTiSAHPLE DIL»  AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO 2 SI6NIFICANT DIGITS
                                                                                                  EPA/RSKERL/ADAtOK

-------
                                               ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
                                                         FOR.
                                                            LIST,LST?2247
 PROJECT:   OLD INGERS SAMPLES  COLLECTED 4/15/86
           P9.13

 CONCENTRATION IN!   NBAS DRY  YT,
»TE
[ME
tt.NO.
LEHENT
NA
K
CA
MS
FE
HN
CO
HO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
AG
TL
PB
LI
SR
V
BA
B
TI
22MAY86
18102
3051
VALUE
119.
1020.
5060.
2440.
14900.
495.
6.93
1.4
13700.
<17.
<11.
0.27
0.83
8.0
18.3
12.5
37.5
<,9
<4.
13.9
8.41
25.2
32.8
202.
3.8
266.
STDV
t/-
3051
VALUE
12.
100.
500.
240.
1400.
49,
0.91
1.2
1300.
17.
11.
0.27
0,27
2.3
1.8
1.3
4.0
0.92
4.3
4.8
0.90
2.5
4.0
20,
1.2
26.
22MAY86
18J04
3052
VALUE
153.
1620.
5150.
2650.
17200.
545.
8.06
3.5
18900.
<23.
<13.
<.2
1.06
10.5
24.7
14.4
41.3
<»9
<5.
20,2
11.3
26.9
41,9
242.
6.6
367.
STDV
+/-
3052
VALUE
16.
160.
510,
260.
1700.
54.
0,96
1.8
1800.
23.
13.
0.28
0.29
2.6
2.4
1.5
4.5
0.96
5.6
6.5
1.1
2,6
5.0
24.
1.5
36.
22MAY86
is:os
3052D
VALUE
129.
1050.
4160.
2450.
17500.
824.
10.4
3.2
15200.
<18.
<13.
<.2
1.08
11.6
20,8
14.8
40.9
<.9
<4«
22.0
9.48
22.4
37.1
258,
2.9
188.
STDV
*/-
2052DUP
VALUE
13.
100.
410.
240.
1700.
81.
1,1
1.5
1500.
18.
13.
0.28
0.28
2.7
2.0
1.5
4.4
0.96
4,7
5.9
0.95
2.2
4.5
25.
1.2
18.
22NAY86
18107
3053
VALUE
125.
1130.
4620.
2330.
13900.
354.
5.83
1.4
14800.
<18»
<11.
<.2
0.78
7.8
19.2
11.6
35.4
<.9
<4.
14.2
8.93
23.9
31.9
181,
4.6
292,
STDV
f/-
3053
VALUE
13.
110,
460,
230,
1300.
35.
0.98
1,3
1400.
18,
11,
0.29
0.30
2,2
1,9
1,2
3.8
0.98
4.6
5,0
0.97
2.3
3.9
IB.
1.3
29.
< VALUE«LI«T OF DETECTION DETERMINED BY  INSTRUMENT SENSTiSANPLE OILf AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO  2 SIGNIFICANT DIGITS
                                                                                                 EPA/R$KERL/ADA»OK

-------
                                               ELEMENT*! CONSTITUENTS ANALYSIS BY  ICAP
                                                         FOR*
                                                           LIST.LSTJ2247
PROJECT:  OLB INGERS SAMPLES COLLECTED vis/86
          P9.13
CONCENTRATION IN!  KG/KG DRY «T,
DATE
TIME
TA6.NO.
ELEMENT
NA
K
CA
H6
FE
MN
CO
NO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
AG
TL
PB
LI
SR
V
BA
B
TI
* ••*• ii^_i
22HAY86
17:53
3047
VALUE
130.
1340.
4420.
2580.
17100.
552.
7.31
<1.
18500.
<22.
<13.
<.2
1.01
9.0
21.7
13.7
36.9
<",?
<5.
15.5
9.91
23.6
42,2
223.
6.1
359.
STDV
*/-
3047
VALUE
13.
130.
440.
250.
1700.
54.
0.98
1.6
1800.
22.
13.
0.28
0.30
2.6
2.1
1.4
4.0
0.99
5.5
6.0
0.99
2.3
5.0
22.
1.5
35.
                                       22MAY86
                                       17134
                                        3048

                                        VALUE

                                        164.
                                       1450.
                                       5660.
                                       2680.
                                      15800.
                                        281.
                                          5.10
                                          3.4
                                      16400.
                                        <20.
                                          0.89
                                          9.1
                                         22.4
                                         13.2
                                         39.6
                                         16.3
                                         10.0
                                         28.7
                                         36.6
                                        195.
                                          5.1
                                        320.
STJV
 */-
  3048

  VALUE

   17.
  140.
  560.
  260.
 1500.
   27.
    0.91
    1.6
 1600.
   20.
   12.
    0.26
    0.28
    2.4
    2.2
    1.4
    4.3
    0.91
    5.0
    5.6
    1.0
    2.8
    4.4
   19.
    1.4
   32.
 22MAY86
 17J59
  3049

  VALUE

  161.
 1330.
 7290.
 2560.
13900.
  236.
    4.64
    2.0
15500.
    0.79
    7.8
   20.2
   11.7
   37.4
   12.3
    9.64
   39.0
   32.2
  179,
    6.8
  298.
                DETECTION KTERHINED IT INSTRUMENT SENSTrSAHPLE DILi AND MATRIX  INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT  DIBITS
STW
*/-
3049
VALUE
16.
130.
720.
250.
1300.
23.
0.92
1.4
1500.
18.
10.
0.27
0.28
2.2
2.0
1.2
4.0
0.92
4.8
5.0
0.97
3.9
3.9
18.
1.3
29.
•w • i ITI~ m rrm i~i tn
22HAY86
ia:oi
3050
VALUE
149.
1530.
4760.
2540.
14800.
273.
4.86
1.5
17000.
<20.
<11.
<.2
0.86
7.4
20.5
11.4
35.0
<,9
<5.
12.5
9.9
26,2
35.8
170.
11.3
388.
STDV
*/-
3050
VALUE
15.
150.
470.
250.
1400.
26.
0.99
1.5
1700.
20.
11.
0.29
0.31
2.3
2.0
1.2
3.8
0.99
5.2
5.4
1.0
2.6
4.3
17.
1.7
38.
                                                                                                EPA/RSKERL/ADArOK

-------
                                                ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
                                                          FOR:
                                                             LIST.LSTI2247
 PROJECT:  OLB INGERS SAMPLES COLLECTED 4/15/86
           P?.13


 CONCENTRATION IN:   MB/KB DRY WT,
BATE
TIME
TAG, NO.
ELEMENT
NA
K
CA
M6
FE
m
CO
NO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
22MAY86
17:47
3043
VALUE
108.
1020.
3620.
2190.
144CO.
279.
4.8
<1.
14500.
<18»
<11.
<.2
0.74
6.9
17.5
10.4
32.1
<1.
<4.
13.7
7.73
19.1
31,0
158.
3.2
237.
STDV
t/-
3043
VALUE
11.
100.
360.
220.
1400.
27.
1.0
1.2
1400.
18.
11.
0.29
0.30
2.3
1.7
1.1
3.5
1.0
4,5
4.9
0.99
1.9
3.8
IS.
1.3
23.
22MAY86
17!48
3044
VALUE
174.
1720.
4900.
2690.
15300.
294.
4.96
<1.
18400.
<22.
<12.
<»2
0.91
8.5
22.9
12.7
37.3
<.8
<5.
11.7
11,4
26.7
37.1
183.
6.2
383.
STDV
f/-
3044
VALUE
18.
170.
490.
270.
1500.
29.
0.88
1.5
1800.
22.
12.
0.25
0.27
2.3
2.2
1.3
4.0
0,87
5.5
5.6
1,1
2,6
4.5
18.
1.5
38.
22MAY86
17!5
3045
VALUE
132.
1280.
5010.
2590.
16900.
300.
4.6
<1.
16800.
<20.
<13.
<»2
0.89
8.0
20.6
12.8
37.7
<1.
<5.
13.2
9.34
24.7
40.4
171.
6.2
338.
STDV
+/-
3045
VALUE
14.
120.
500.
260.
1600.
29.
1.0
1.4
1600.
20.
13.
0.29
0.31
2.6
2.0
1.3
4.1
1.0
5,2
5.5
0.99
2.4
4.9
17.
1.5
'33.
22HAYB6
17J51
3046
VALUE
137.
1340.
3800.
2640.
17000.
484.
6.86
<1.
17900.
<21.
<13.
<.2
0.94
7.9
21.3
13.2
35.0
<,9
<5.
15.0
10.5
21.6
38.7
192.
6.2
349,
STDV
*/-
3046
VALUE
14.
130.
380.
260.
1700.
47.
0.95
1.5
1700*
21.
13.
0.27
0.29
2.6
2.1
1.4
3.8
0.96
5.5
5.9
1.0
2.1
4.7
19.
1.5
34.
< VALUE«LINIT OF DETECTION DETERMINED BY INSTRUMENT SENSTiSAHPLE DILi AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO 2 SI6NIFICANT BI6ITS
                                                                                                  EPA/RSKERL/ADA.OK

-------
                                                ELEMENTAL CONSTITUENTS ANALYSIS  BY  ICAP
                                                          FOR:
                                                            LIST.LSTJ2247
 PROJECT:  OLD INGERS SAMPLES COLLECTED 4/is/w
           P9.13
 CONCENTRATION IN*.   M6/K6 DRY UT.

DATE        22Mm      STDV
TIME        17M          */-
TA6.NO.      3040          3040

ELEMENT      VALUE         VALUE
22MAY86
17J42
 3041

 VALUE
STDV
 */-
  3041

  VALUE
22HAY86
17J43
 3042

 VALUE
                                                                                STDV
                                                                                 +/-
                                                                                  3042

                                                                                  VALUE
NA
K
CA
M6
FE
HN
CO
HO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
152.
1400.
4940.
2350.
14000.
227.
3.87
<1,
16500.
<20.
<11.
<.2
0.84
7.5
19.6
11.2
33.8
<,8
<4.
10.8
9.92
26.?
33.3
165.
6.0
337.
15.
140.
490.
230.
1400.
22.
0.81
1.3
1600.
20.
11.
0.23
0.25
2.1
1.9
1.2
3.6
0.81
4.9
5.1
0.99
2.7
4.0
16.
1.3
33.
115.
1000.
4050.
2300.
14500.
260.
5.55
<1.
15800.
<19.
<11.
<.2
0.76
7.3
19.3
10.8
34.6
<.9
<4.
13.0
8,48
20.6
31.0
165.
3.2
256.
12.
100.
400.
230.
1400.
25.
0.99
1.3
1500.
19.
11.
0.29
0.30
2.3
1.9
1.1
3.7
0,99
4.8
5.1
0.97
2.0
3.8
16.
1.3
25.
133.
1270.
5640.
2470.
14600.
241.
4.38
<1.
16300.
<20.
<11.
<.2
0.83
7.3
19.6
11.9
38.2
<.9
<5.
12.1
9.22
28.3
33.8
164.
4.9
331.
14.
120.
560.
240.
1400.
23.
0.97
1.4
1600.
20.
11.
0.28
0.30
2.3
1.9
1.2
4.1
0.97
5.0
5.2
0.95
2.8
4.1
16,
1.4
33.
129.
1200.
6010.
2470,
14500.
240.
4.22
<1.
15700.
<19.
<11.
<.2
0.81
7.1
19.4
11.4
37.3
<.9
<4.
13.1
8.95
28.1
33.0
161.
4.4
317.
13.
120.
600.
240.
1400.
23.
0.99
1.3
1500.
19.
11.
0.29
0.30
2.3
1.9
1.2
4.0
0.99
4.9
5.1
0.97
2.8
4.0
16.
1.4
31.
< VALUE=LIMIT OF DETECTION DETERNINEB BY INSTRUMENT SENSTrSAMPLE DILf  AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO 2 SIGNIFICANT DIBITS
                                                                                                  EPA/RSKERL/ADAfOK

-------
                                                 ELEMENTAL CONSTITUENTS ANALYSIS  IT  ICAP
                                                           FOR:
                                                             LIST.LSTJ2243
 PROJECT:  OLD  INGERS SITE  SAMPLES COLLECTED 3/10/86
           P13.9
 CONCENTRATION  IN: K6/K6 DRY «T.


 MTE        22NAY86      STDV

 TIME        17:26         +/-
 rAG.NO.      2487          2487


 •LENENT      VALUE         VALUE
VALUE
VALUE
VALUE
VALUE
m
K
CA
H6
FE
m
CO
MO
AL
AS
SE
CD
DE
CU
CR
NI
ZN
A6
TL
PD
LI
SR
V
BA
D
TI
155.
1660.
5180.
2700.
15400.
395.
5.89
5.3
17700.
<21.
<12.
<.2
0.92
9.7
23.9
13.4
44.1
<.9
<5.
16.1
11.6
29.6
3S.5
242.
5.7
368.
16.
160.
510.
270.
1500.
39.
0.99
1.8
1700.
21.
12.
0.29
0.30
2.4
2.3
1.4
4.7
0.99
5.3
5.8
1.1
2.9
4.3
24.
1.5
36.
144.
1640.
5380.
2690.
16000.
538.
7.9
5.5
17600.
<21,
<12.
<.2
0.93
9.9
24.9
14.6
42.3
<1.
<5.
19.6
• 11.4
28.2
37.4
257.
7.1
366.
15.
160.
530.
270.
1600.
53.
1.0
1.9
1700.
21.
12.
0.29
0.31
2.5
2.4
1.5
4.5
1.0
5.5
6.1
1.1
2.8
4.5
25.
1.5
36.
< VALUE*LIMIT OF DETECTION DETERMINED DY INSTRUMENT SENSTtSAHPLE DILr AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO 2 SIBNIFICANT DIGITS
                                                                                                  EPA/RSKERL/ADA»OK

-------
                                                ELENENTAL CONSTITUENTS ANALYSIS BY  ICAP
                                                          FOR:
                                                             LIST.LSTJ2243
  PROJECT:  OLD  INGERS SITE SAMPLES COLLECTED 3/10/86
           P13.9
  CONCENTRATION IN! NG/KG DRY HT.

 MTE        22MAY86      STDV           22MAY86      STBV           22NAY86
 FINE        17J2          t/-           17J22        */-           17:23
 FAG.NO.      2483          2483          2484          2484          2485

 ELEMENT      VALUE         VALUE         VALUE         VALUE         VALUE
< VALUE«LINIT OF DETECTION DETERMINED BY INSTRUMENT SENSTiSANPLE DILt AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
STDV
 */-
  2486

  VALUE
NA
K
CA
M6
FE
HN
CO
MO
AL
AS
SE
CD
BE
CU
CR
HI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
110.
1210.
4440.
2370.
16900.
557.
7.46
<1.
15000.
<18.
<13.
<.2
0.87
7.2
19.0
11.2
34.4
<1.
<4.
12.8
8.46
23.2
33.1
217.
5.5
326.
11.
120.
440.
230.
1600.
55.
0.99
1.3
1500.
18.
13.
0.29
0.30
2.6
1.8
1.2
3.8
1.0
4.8
5.1
0.97
2.3
4.1
21.
1.4
32.
189.
1050.
16500.
2400.
14500.
472.
6.4
<1.
14700.
<18.
<11.
<.2
0.77
8.1
18.0
12.8
34.8

-------
                                               ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
                                                         FOR:
                                                            LIST.LSTJ2237
 PROJECT:  OLD INGERS  SITE SAMPLES COLLECTED 1/22/8*
           P9.9

 CONCENTRATION IN! MS/KG DRY HT.

DATE        2mm      STDV           21MAY86      STDV
TIKE        20U4         */-           20:15         V-
TA6.NO.       1947          1947          1948          1948

ELEMENT      VALUE          VALUE         VALUE         VALUE
VALUE
VALUE
NA
K
CA
M6
FE
HN
CO
MO
AL
AS
SE
CD
BF
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
134.
1470.
S210.
2870.
17600,
375.
3.78
<1.
18000.
<22,
<13.
<.2
0.90
8.8
22.7
14.3
S3.0
<,9
<5.
12.7
9.09
26.9
38.5
192.
4.9
381.
14.
140.
520.
280.
1700.
37.
0.94
1.5
1800.
22.
13.
0.28
0.30
2.7
2.2
1.4
5.7
0.96
5.4
5.7
0.93
2.6
4,7
19.
1.5
38.
121.
1100.
6440.
2410.
14500.
545.
8.16
<1.
14900.
<1B.
<11.
<,2
0,82
7.9
19.0
12.7
37.5
<,8
<4.
13.6
7.68
29.5
32.4
187.
3.4
248.
13.
110.
640.
240.
1400.
54.
0.87
1.3
1400.
18.
11.
0.25
0.26
2.2
1.8
1.3
4.1
0.88
4.8
5.0
0.86
2.9
3.9
18.
1.2
24.
164.
981.
19300.
2300.
13200.
330.
5.6
<1.
13800.
<17.
<10.
<.2
0.67
7,4
17,1
11.4
37.3
<1,
<5.
13.1
6.83
80.2
28.9
159.
1,8
239.
t7.
99.
1900.
230.
1300.
32.
1.0
1.2
1300.
17.
10.
0.29
0.30
2.1
1,6
1.2
4.0
1.0
5.7
4.7
0.99
8.0
3.5
16.
1.4
24.
< VALUE*LIHIT OF 1ETECTION BETERMINED BY INSTRUMENT  SENSTfSAMPLE DILr AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO  2  SIGNIFICANT DIBITS
                                                                                                 EPA/RSKERLWAfOK

-------
                                                 ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
                                                          FOR!
                                                             LIST.LSTI2237

  PROJECT:   OLD  INSERS  SITE SAMPLES COLLECTED  1/22/86
           P9.9

  CONCENTRATION IN! M6/K6 DRY UT.

 DATE        21MAY86      STDV           21NAY86      STDV           21HAY86      STDV           21MAY86      STDV
 TIME        20J08         t/-           20J09        f/-           20M1         +/-           20J12         +/-
 TA6.NO.      1943          1943          1944          1944          1945          1945          1946          1946

 ELEMENT      VALUE         VALUE         VALUE         VALUE         VALUE         VALUE         VALUE         VALUE
MA
K
CA
H6
FE
MN
CO
MO
AL
AS
SE
CO
BE
cu
CR
HI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
134.
1440.
4740.
2850.
15100.
290.
4,9
<1.
16600.
<20.
<11,
<.3
0.81
8.2
21.1
12.5
38.4
<1.
<5.
11.1
8.6
24.8
34.4
170.
5.5
317.
14.
140.
470.
280.
1500.
28.
1.0
1.4
1600.
20.
11.
0.30
0.31
2.4
2.0
1.3
4.2
1.0
5.1
5.2
1.0
2.4
4.1
17.
1.4
31.
105.
1070.
4240.
2380.
12400.
254.
4.9
<1.
13700.
<16.
<9.
<.2
0.71
6.8
16.4
11.3
34.4
<1.
<4.
12.0
7.05
21.3
28.5
145.
2.0
261.
11.
100.
420.
240.
1200.
25.
1.0
1.1
1300.
16.
9.9
0.29
0.30
2.0
1.6
1.1
3.7
1.0
4.0
4.5
0,99
2.1
3.4
14.
1.3
26.
125.
1260.
4610.
2490.
14700.
333.
5.3
4.8
15600.
<1*.
<11.
<.2
0.91
9.0
21.9
12.1
41.5
<1.
<4.
16.4
8.51
24.5
32.3
220.
6.5
264.
13.
120.
460.
250.
1400.
32.
1.0
1.6
1500.
18.
11.
0.29
0.30
2.3
2.1
1.2
4.5
1.0
4.6
5.4
0.99
2.4
3.9
22.
1.3
26.
144.
1440.
7740.
2540.
14500.
243.
5.2
3.7
16700.
<20.
<11.
<.3
0.92
9.1
22.5
11.9
41.6
<1.
<5.
15.7
8.9
42.6
32.3
214.
1.6
279.
IS.
140.
770.
250.
1400.
23.
1.0
1.6
1600.
20.
11.
0.30
0.31
2.3
2.2
1.2
4.5
1.0
5.2
5.6
1.0
4.2
3.9
21.
1.4
28.
< VALUE4.IMIT OF DETECTION DETERMINED IY INSTRUMENT SENSTtSAMPLE DILt AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
                                                                                                  EPA/RSKERL/ADAiOK

-------
                                                ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
                                                          FOR:
                                                             LIST.LSTI2237
 PROJECT:  on INGERS SITE SAMPLES COLLECTED 1/22/8*
           P9.9

 CONCENTRATION IN! H6/KS DRY IfT.
                         STDV
                          */-
                           1940

                           VALUE

                            14.
                           110.
                           920.
                           220.
                          1300.
                            31.
                             0.94
                             1.3
                          1500.
                            18.
                            10.
                             0.28
                             0.28
                             2.1
                             1.7
                             1.1
                             3.9
                             0.95
                             4.9
                             5.0
                             0,93
                             3.4
                             3.7
                            17.
                             1.2
                            23.
DATE
TIME
TA6.NO.
ELEMENT
NA
K
CA
M6
FE
MM
CO
HO
AL
AS
SE
CD
BE
CD
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
21HAY86
20:02
1940
VALUE
139.
1120,
9200.
2230.
13500.
318.
5.12
<1.
15400.
<18.
<10.
<,2
0.78
7.2
18.0
11.2
35.7
<,9
<4.
12.7
7,76
34.9
30,9
176.
4.4
233.
21MAY86
20:03
1940DUP
VALUE
127.
1380,
4510.
2340,
14200.
298.
4.5
<1.
16800.
<20.
<11.
<.3
0.86
7,6
19.7
11.5
36.3
<1.
<4.
12.5
8.3
24.5
34,7
179.
10.0
312.
STDV
V-
1940DUP
VALUE
13.
130,
450.
230.
1400.
29.
1.0
1.4
1600.
20.
11.
0.30
0.31
2.2
1.9
1.2
3.9
1.0
4.9
5.3
1.0
2.4
4,1
IB.
1.5
31.
 21NAY86
 20!05
  1941

  VALUE

  149.
 1450.
 4240.
 2550.
14000.
  227.
    4.22
    2.6
16300,
    0.81
    8.1
   22.3
   11.8
   39,1
   12.9
    8.55
   24.4
   32,5
  187.
    7.6
  332.
< VALUE*LIMIT OF DETECTION DETERMINES BY INSTRUMENT SENSTrSAHPLE DILi AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
STDV
\l-
1941
VALUE
15.
140.
420.
250.
1400.
22.
0.98
1.5
1600.
19.
11.
0.28
0.30
2.2
2.2
1.2
4.2
0.98
4.9
5.2
0.96
2.4
3.9
18.
1.4
33.
21MAY86
20:06
1942
VALUE
116.
1350.
4320.
2410.
15300.
277.
4,7
<1.
16800.
<20.
<12.
<.2
0.83
7.2
20.9
12.1
34.0
<1.
<5.
13.2
8.00
23.4
36.1
165.
7.9
330.
STDV
*/-
1942
VALUE
12.
130.
430.
240.
1500.
27.
1.0
1.4
1600.
20.
12.
0.29
0,31
2.4
2.0
1,2
3.7
1.0
5.1
5.4
0,99
2.3
4,3
16.
1,4
33.
                                                                                                  EPA/RSKERL/ADAfOK

-------
                                                 ELEHENTM. CONSTITUENTS ANALYSIS BY ICAP
                                                          FOR:
                                                             LIST.LSTI2237
  PROJECT:   OLD  INGERS SITE SAMPLES COLLECTED  1/22/86
           P9.9
 CONCENTRATION INI KG/KG DRY MT.

 MTE        21MAY86      STDV           21HAY86      STDV           21MAY86      STDV
 TIME        19551         */-           19:53         f/-           19154         */-
 FAG.NO.      1934          1936          1937          1937          1938          1938

 ELEMENT      VALUE         VALUE         VALUE         VALUE         VALUE         VALUE
NA
K
CA
N6
FE
m
CO
m
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
118.
1260.
4300.
2370.
HIM.
269.
4.82
<1.
15800.
<19.
<11.
<.2
0.79
7.0
18.5
11.3
34.4
<.9
<4.
11.9
7,73
21.7
32.3
165.
3.0
300.
12.
120.
430.
230.
1400.
26.
0.90
1.3
1500.
19.
11.
0.26
0.27
2.2
1.8
1.1
3.7
0.90
4.7
5.0
0.88
2.1
3.9
16.
1.3
30.
148.
1260.
5290.
2530.
14200.
277.
5.07
<1.
15700.
<19.
<11.
<.2
0.80
7.5
20.3
12.6
37.9
<.8
<4.
10.5
8.06
26.8
31.5
167.
3.4
256. .
15.
120.
530.
250.
1400.
27.
0.86
1.3
1500.
19.
11.
0.25
0.26
2.2
2.0
1.3
4.1
0.86
4.6
4.9
0.84
2.6
3.8
16.
1.2
25.
126.
1450.
4840.
2650.
15900.
280.
5.1
<1.
17400.
<21.
<12.
<.3
0.84
8,0
22.1
14.8
39.1
<1.
<5.
11.2
8.8
26.5
36.5
172.
6.0
374.
13.
140.
480.
260.
1500.
27.
1.0
1.5
1700.
21.
12.
0.30
0.31
2.5
2.1
1.5
4.2
1.0
5.3
5.4
1.0
2.6
4.4
17.
1.6
37.
227.
1520.
18600.
2390.
13700.
300.
5.6
<1.
16900.
<20.
<10.
<.3
0.79
7.2
19.3
10.9
34.4
<1.
<6.
11.2
8.6
72.8
33.4
175.
14.8
344.
23.
150.
1800.
240.
1300.
29,
1.0
1.4
1600.
20.
10.
0.30
0.31
2.2
1.9
1.1
3.7
1.0
6.2
5.3
1.0
7.2
4.0
17.
2.1
34.
< VALUE=LIMIT OF DETECTION DETERMINED DY INSTRUMENT SENSTiSAMPLE DILi AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
                                                                                                  EPA/RSXERL/ADAfOK

-------
                                                ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
                                                          FOR!
                                                             LIST.LSTJ2237
  PROJECT:   OLD INBERS SITE SAMPLES COLLECTED 1/227*6
            P9.9
CONCENTRATION IN! M8/K6 DRY NT.
DATE
TIME
TA6.M.
ELEMENT
NA
K
CA
H6
FE
m
CO
MO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
21NAY86
19M5
1932
VALUE
• 129.
1020.
3910.
2370.
13100.
235.
4.55
<1.
14200.
<17.
<10.
<.2
0.66
6.7
17.7
10.7
32.4
<,8
<4.
10,1
6.12
20,4
27.5
154.
8.5
242.
STDV
+/-
1932
VALUE
13.
100.
390.
230.
1300.
23.
0.89
1.2
1400.
17.
10.
0.26
0.27
2.0
1.7
1.1
3.5
0.89
4.3
4.5
0.87
2.0
3.3
IS.
1.2
24.
                                        21MAY86
                                        19J47
                                         1933

                                         VALUE

                                         185.
                                        1440.
                                        9350.
                                        2590.
                                       14900.
                                         293.
                                           5.17

                                       17100.
                                           0.79
                                           7.9
                                          21,7
                                          13.1
                                          55.9
                                          10.9
                                           8.64
                                          42.1
                                          34.2
                                        183.
                                           4.6
                                        363.
STDV
 */-
  1933

  VALUE

   19.
  140.
  930.
  260.
 1400.
   28.
    0.99
    1.4
 1700.
   21.
   11.
    0.29
    0.30
    2.3
    2.1
    1.3
    5,9
    0.99
    5.4
    5.3
    0.96
    4.2
    4.1
   18.
    1.5
   36.
 21MAY86
 19!48
  1934

  VALUE

  145.
 1590.
 7450.
 2610.
15400.
  372.
    5.8

18300.
  <22.
    0.90
    8.0
   21.4
   12.8
   36.4
   13.3
    8.99
   35.6
   37.4
  198.
    5.3
  382.
STDV
*/-
1934
VALUE
IS.
160.
740.
260.
1500.
36.
1.0
1.5
1800.
22.
12.
0.29
0.31
2.4
2.1
1.3
4.0
1.0
5.5
5.8
0.98
3.5
4.5
19.
1.6
38.
21HAY86
19:5
1935
VALUE
156.
1630.
4500.
2570.
17400.
366.
6.6
<1.
22000.
<27.
<13.
<.2
1.03
8.9
24.4
13.8
37.7
<1,
<6.
12.6
9,79
27.5
40,8
. 200.
7.8
385.
< VALUE»LIMIT OF DETECTION DETERMINE BY  INSTRUMENT SENST,SAMPLE DILi AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO 2 SIGNIFICANT BI6ITS
STDV
 */-
  1935

  VALUE

   16.
  160.
  450.
  250.
 1700.
   36.
    1.0
    1.8
 2200.
   27.
   13.
    0.29
    0.31
    2.7
    2.4
    1.4
    4.2
    1.0
    6.3
    6.6
    0.99
    2.7
    4.9
   20.
    1.6
   38.
                                                                                                 EPA/RSKERL/ADAfOK

-------
                                               ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
                                                         FOR:
                                                            LIST.LSTI2237
PROJECT:  OLD IMBERS SITE SAMPLES COLLECTO 1/22/8*
          P9.9
CONCENTRATION IN! H6/K6 DRY WT.
BATE
TINE
TA6.NO.
ELEMENT
MA
K
CA
MS
FE
m
CO
MO
AL
AS
SE
CD
BE
OJ
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
j IIAI ttf-A
21HAYB6
19139
1929
VALUE
146.
1250.
6340.
2640.
14700.
349.
6.14
<1.
15900.
<19.
<11.
<.2
0.83
7.9
18.9
12,8
37.4
<.9
<4.
10.7
8.38
28.0
31.4
170,
24.8
278.
VMW ftf tf il'M
STDV
+/-
1929
VALUE
15.
120.
630.
260.
1400.
34.
0.91
1.3
1500.
19.
11.
0.26
0.27
2.3
1.8
1.3
4.1
0.91
4.8
5.0
0.39
2.8
3.8
17.
2.7
27.
TTHM A^WVUCVUf"
21MAY86
19M1
1929DUP
VALUE
121.
1540.
5670.
2740.
15200.
259.
4.60
<1.
17600.
<21.
<11.
<,2
0.85
7,6
21.1
12.4
36.5
<.9
<5.
9.8
9.05
26.0
34.6
177.
9.1
357.
Ik •«• • IM* iiMMl^lil
STDV
+/-
1929DUP
VALUE
13.
150.
560.
270.
1500.
25.
0.95
1.4
1700.
21.
11*
0.28
0.29
2.3
2.0
1.3
4.0
0.95
5.2
5.3
0.93
2.5
4.2
17.
1.5
35.
r Mf*UMV MAMMA f
21MAYB6
19!42
1930
VALUE
122.
1410.
4240.
2510.
16100.
460.
6.2
<1.
18100.
<22.
<12.
<«2
0.90
8.1
21.4
12.8
36.7
<1.
<5.
12.8
8.53
24.5
37.8
199.
5,4
347,
IkVl AUK UAWh
STDV
+/-
1930 t
VALUE
13.
140.
420.
250.
1600.
45.
1.0
1.5
1800.
22,
12,
0.29
0.31
2.5
2.1
1.3
4.0
1.0
5.3
5.7
0.98
2.4
4.5
19.
1.5
34.
21HAY86
19!44
1931
VALUE
140.
1380.
6670.
2220.
13800.
381.
6.36
<1.
16200.
<20.
<10.
<.2
0.81
7.5
19.0
11.2
32.8
<»9
<4.
13.9
7.87
28.7
34.2
184.
3.4
344.
STDV
\l-
1931
VALUE
15.
140.
660.
220.
1300.
37.
0.99
1.3
1600.
20.
10.
0.29
0.30
2,2
1,8
1.1
3.6
0.99
4.9
5.3
0.97
2.8
4.1
18.
1.5
34.
RESULTS ACCURATE TO 2 SIGNIFICANT DIBITS
                                                                                                 EPA/RSKERL/ADAiOK

-------
 PROJECT:  OLB INGERS SITE SAMPLES
          COLLECTED 1/22/84
          P9.1

 CONCENTRATION IN! M6/K6 DRY HT.
                                               ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
                                                         FOR:
                                                            LIST.LSTI2233
                                                    STHV
                                                     */-
                                                      1928

                                                      VALUE

                                                       13.
                                                      130.
                                                      450.
                                                      230.
                                                     1300.
                                                       23.
                                                        0.99
                                                        1.3
                                                     1500,
                                                       19.
                                                       10.
                                                        0.29
                                                        0.30
                                                        2.1
                                                        2.1
                                                        1.2
                                                        3.6
                                                        0.99
                                                        5.0
                                                        5.3
                                                        0.98
                                                        2.3
                                                        3.5
                                                       15.
                                                        1.4
                                                       32.
                                     BY INSTRUMENT SENSTtSAHPLE BILi  ANB MATRIX  INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT BI6ITS
BATE
TIME
TA6.NO.
ELEMENT
m
K
CA
H6
FE
MN
CO
NO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
AC
TL
PB
LI
SR
V
BA
B
TI
S IftAI IIP_i
7MAYB6
10M3
1927
VALUE
136.
1310.
4870.
2540.
14700.
443.
7.4
<1.
15800.
<19.
14.
<,2
0.80
11.0
21.9
13.9
36.4
<1.
5.5
19.1
7.20
25.2
32.0
175.
5.1
307.
VMW Hf nfrrt
STDV
*/-
1927
VALUE
13.
130.
480.
250.
1400.
43.
1.0
1.3
1500.
19.
11.
0.29
0.30
2.3
2.1
1.4
3.9
1.0
5.2
5.6
0.99
2.5
3.9
17.
1.4
30.
tWMAl fcl'll lfcM»M^
7MAY86
10M4
1928
VALUE
135.
1350.
4590.
2320.
13100.
239.
4.75
<1.
15900.
<19.
13.
<,2
0.77
6.1
21.5
11.8
33.9
<.9
<5.
16.5
7.25
23.8
28.9
152.
3.3
323.
& &M TlMITnillM-
7NAY86
10!46
1928BUP
VALUE
152.
1180.
8300.
2270.
12800.
230.
4.48
<1.
15300.
<18,
12.
<,2
0.70
6.0
20.2
11.3
33.7
<.9
<5.
15.5
7.15
45.1
27.3
149.
5.0
259.
•hVt A&Ht MATM1
STDV
*/-
19280UP
VALUE VALUE
IS.
110.
830.
220.
1200.
22.
0.96
1.3
1500.
IB.
10.
0.28
0.29
2.0
1.9
1.2
3.6
0.96
5.1
5.1
0.94
4.5
3.3
14.
1.3
26.
VALUE
                                                                                                EPA/RSXERL/ADAiOK

-------
                                                ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
                                                          FORt
                                                             LIST.LSTJ2233
 PROJECT:  ou INGERS SITE SAMPLES
           COLLECTED 1/22/86
           P9.1

 CONCENTRATION IN! H6/K6 DRY «T.
 CONCENTRATION IN!
DATE
TIME
TA6.NO.
ELEMENT
NA
K
CA
M6
FE
MN
CO
MO
AL
AS
SC
CD
BE
CU
CR
NI
ZN
AG
TL
PB
LI
SR
V
BA
B
TI
* ••&• i^_i <
7HAY86
10:37
1923
VALUE
151.
1300.
5320.
2420.
14600.
531.
8.7
<1.
15700.
<19.
13.
0.51
0.82
6.9
21.4
16.1
36.0
<1.
5.3
17.9
7.57
27.4
31.0
168.
8.1
311.
STDV
\h
1923
VALUE
15.
130.
530.
240.
1400.
52.
1.0
1.3
1500.
19.
11.
0.29
0.30
2.3
2.1
1.6
3.9
1.0
5.1
5.5
0.98
2.7
3.8
16.
1.4
31.
7MAY86
10138
1924
VALUE
131.
1240.
3570.
2220.
13800.
232.
4.62
<1.
16400.
<20.
12.
<,2
0.77
6.4
20.8
12.2
32.2
<.8
5.2
15.0
7.35
20.2
29.2
158.
6.1
277.
fe ^M 9UM4MM m*^\m
STDV
*/-
1924
VALUE
13.
120.
350.
220.
1300.
22.
0.87
1.3
1600.
20.
10.
0.25
0.26
2.1
2.0
1.2
3.5
0.87
5.2
5.4
0.85
2.0
3.6
15.
1.2
27.
V J^VtMt V M A MIU
7MAY86
10!4
1925
VALUE
129.
1440.
4980.
2430.
14400.
232.
4.6
4.8
17100.
<20.
13.
<.2
0.77
8.0
25.7
14.1
40.6
<1.
6.4
21.9
8.06
26.6
30.5
215.
4.3
304.
•hVt AUfh MAVA
STDV
+/-
1925
VALUE
12.
140.
490.
240.
1400.
22.
1.0
1.7
1700.
20.
11.
0.29
0.31
2.3
2.5
1.4
4.3
1.0
5.5
6.1
0.99
2.6
3.7
21.
1.4
30.
7HAY86
10M1
1926
VALUE
148.
1420.
5180.
2560.
14400.
295.
5.81
<1.
16600.
<20.
13.
<.2
0.83
6.7
21.7
12.0
37.8
<»9
<5.
17.1
7.80
25.2
31.4
170.
6.0
316.
< VALUE-LIMIT OF DETECTION DETERMINED BY INSTRUMENT SENSTtSAHPLE DIL» AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
                                                                                                             STDV
                                                                                                              f/-
                                                                                                               1926

                                                                                                               VALUE

                                                                                                                14.
                                                                                                               140.
                                                                                                               510.
                                                                                                               250.
                                                                                                              1400.
                                                                                                                29.
                                                                                                                 0.90
                                                                                                                 1.4
                                                                                                              1600.
                                                                                                                20.
                                                                                                                11.
                                                                                                                 0.26
                                                                                                                 0.27
                                                                                                                 2.2
                                                                                                                 2.1
                                                                                                                 1.2
                                                                                                                 4.0
                                                                                                                 0.90
                                                                                                                 5.3
                                                                                                                 5.6
                                                                                                                 0.88
                                                                                                                 2.5
                                                                                                                 3.8
                                                                                                                17.
                                                                                                                 l.*4
                                                                                                                31.
                                                                                                  EPA/RSKERL/AOAiOK

-------
                                                ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
                                                          FOR:
                                                             LIST.LST52233
 PROJECT:  OLD INSERS SITE SAMPLES
           COLLECTED 1/22/86
           P9.1

 CONCENTRATION IN!  N6/K6 DRY VT.
DATE
TIME
TA6.NO.
ELEMENT
NA
K
CA
MS
FE
HN
CO
HO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
7NAY86
10:28
1919
VALUE
135.
11BO.
4090.
2490.
14100.
407.
6.56
<1.
14700.
<17.
11.
0,37
0.75
6.5
18.9
13.0
34.0
<.9
5,0
15.9
7.37
21.1
28.5
171.
3.2
236.
STDV
+/-
1919
VALUE
13.
110.
400.
250.
1400.
40.
0.95
1.2
1400.
17.
11.
0.28
0.28
2.2
1.8
1.3
3.6
0.95
4.8
5.1
0.93
2.0
3.5
17.
1.2
23.
7HAY86
10:3
1920
VALUE
160.
1610.
5450.
2840.
14300.
250.
4.87
<1.
17300.
<21.
12.
<.2
0.82
6.9
28.4
12.9
52.6
<.8
<5.
16.2
8.35
27.1
30.7
167.
5.3
314.
STW
*/-
1920
VALUE
16.
160.
540.
280.
1400.
24.
0.87
1.4
1700.
21.
11.
0.25
0.27
2.2
2.8
1.3
5.5
0.87
5.5
5.7
0.85
2.7
3.8
16.
1.3
31.
7MAY86
10:31
1921
VALUE
159.
1620.
7730.
2720.
15700.
289.
5.51
2.3
18400.
<22.
14.
<.2
0.93
8.0
26.9
13.9
38.9
<.9
<5.
18.0
8.95
38.1
34.0
195.
6.1
342.
STDV
*/-
1921
VALUE
15.
160.
770.
270.
1500.
28.
0.97
1.6
1800.
22.
12.
0.28
0.29
2.4
2.6
1.4
4.2
0.97
5.8
6.1
0.94
3.8
4.2
19.
1.5
34.
THAW
10:34
1922
VALUE
147.
1550.
4670.
2500.
14200.
243.
4.38
<1.
17500.
<21.
12.
0.36
0.84
6.8
22.9
13.5
35.1
<.8
5.6
15.7
8.23
26.5
31.2
165.
6.7
354.
STDV
*/-
1922
VALUE
14.
150.
460.
250.
1400.
23.
0.89
1.4
1700.
21.
11.
0.26
0.27
2.2
2.2
1.4
3.8
0.89
5.5
5.7
0.87
2.6
3,8
16.
1.4
35.
< VALUE«UNIT OF DETECTION DETERMINED BY INSTRUMENT SENSTiSAMPLE OIL.  AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO  2 SIGNIFICANT DIGITS
                                                                                                  EPA/RSKERL/ADAtOK

-------
  PROJECT:  ou DKJERS SITE SAMPLES
           COLLECTED 1/22/84
 CONCENTRATION IN: KG/KG DRY BT.
                                                ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
                                                          FOR:
                                                             LIST.LSTJ2233
DATE
TIME
TA6.NO.
ELEMENT
NA
K
CA
H6
FE
HN
CO
HO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B.
TI
7MAY86
10:21
1916
VALUE
140.
1200.
5540.
2330.
12900.
237.
4.56
<1.
15000.
<18.
11.
<.2
0.75
6.3
17,4
10.9
32.8
<<8
<4.
13.4
7.31
27,4
27.1
147.
5.4
275.
STDV
•f/-
1916
VALUE
14.
120.
550.
230.
1200.
23.
0,88
1,2
1500.
18.
10.
0.26
0.27
2.0
1.7
1.1
3.5
0.88
4.8
4.9
0,86
2,7
3.3
14.
1.2
27.
7NAY86
10:23
1917
VALUE
156.
1310.
4500.
2540.
14400.
259.
4.8
<1,
16300.
<20.
13.
<.2
0.77
6.5
21.5
12.1
35.2
<1.
<5.
14.8
7.69
23.6
30.0
156.
7.4
305.
STDV
*/-
1917
VALUE
15.
130.
450.
250.
1400.
25.
1.0
1.4
1600.
20.
11.
0.29
0.30
2.3
2.1
1.2
3.8
1.0
5.1
5.4
0.99
2.3
3.7
IS.
1.4
30.
7NAY86
10:24
1917DUP
VALUE
166.
1470.
4330.
2630.
14600.
231.
4.79
<1»
17000.
<20.
15.
<.2
0.87
7.0
22.0
12.4
35.6
<.9
<5.
15.0
8.23
24.1
30.7
163.
7.2
325.
STDV
#/-
1917DUP
VALUE
16.
140.
430.
260.
1400.
22.
0.98
1.4
1700.
20.
11.
0.28
0.30
2.3
2.1
1.3
3.8
0.98
5.4
5.5
0.96
2.4
3.8
16.
1.4
32.
7HAY86
10126
1918
VALUE
164.
1380.
5000.
2390.
13600.
210.
4.41
<1»
16000.
<19.
12.
<.2
0.79
6.6
21,3
12.9
35.3
<.9
<5.
13.5
7,93
26,1
29,0
151.
7,5
320.
STDV
*/-
1918
VALUE
16.
130.
500.
260.
1300.
20.
0.96
1.3
1600.
19,
10.
0.28
0.29
2,1
2.1
1.3
3.8
0.96
5.0
5.2
0.94
2.6
3,6
15.
1,4
32.
< VALUE*IMIT OF DETECTION BETERMINED BY INSTRUMENT SENSTiSAMPLE DILi AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO  2 SI6NIFICANT  DIBITS
                                                                                                  EPA/RSKERL/ADA.OK

-------
 PROJECT:  OLD INGERS SITE SAMPLES
           COLLECTED 1/22/84
           P9.1

 CONCENTRATION INI M6/K6 DRY VT.
                                                ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
                                                          FOR:
                                                             LIST.LSTI2233
DATE
TIME
TA6.NO.
ELEMENT
NA
K
CA
M6
FE
MN
CO
MO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
7HAY86
10:i
1912
VALUE
148.
1360.
4300.
2300.
14604.
215.
3.5
<1.
18700.
<22.
13.
<.3
0.79
7.3
22.9
12.1
34.4
<1»
<5.
14.6
8.6
25.5
30.6
172.
5.9
291.
STDV
*/-
1912
VALUE
14,
130.
430.
230.
1400.
21.
1.0
1.5
1800.
22.
11.
0.30
0.31
2.3
2.2
1.3
3.7
1.0
5.8
5.9
1.0
2.5
3.8
17,
1.4
29.
7MAY86
10:13
1913
VALUE
152.
1170.
5250.
2450.
14000.
220.
4.18
<1.
15200.
<18.
14.
0.27
0.71
6.5
21.1
11.4
35.4
<«9
<4.
14.7
7.55
27.0
27.0
149.
4.8
225.
STDV
*/-
1913
VALUE
15.
110.
520.
240.
1400,
21.
0.91
1.2
1500.
18.
11.
0.27
0,27
2.2
2.0
1.2
3.8
0.92
4.8
5.1
0.90
2.6
3.4
15.
1.2
22.
7MAY86
10! 15
1914
VALUE
166.
1470.
6770.
2410.
14600.
328.
5.57
<1.
17800.
<21.
14.
<»2
0,83
7.5
23.7
13.0
40.3
<»9
<5.
16.5
8.04
34.0
32.8
180.
7.4
328.
STDV
+/-
1914
VALUE
16.
140.
670.
240.
1400,
32.
0.96
1.5
1700.
21.
11.
0.28
0.29
2.3
2.3
1.3
4.3
0.96
5.6
5.8
0.94
3.3
4.0
18.
1.4.
32.
7MAY86
10I17
1915
VALUE
165,
1530.
5950.
2310.
16300.
364,
6.01
<1.
18400.
<22.
14.
0.27
0.93
7.8
23.7
12.6
35.4
<»8
<5.
18.3
8.59
32.8
36.0
208.
9.5
339.
STDV
*/-
1915
VALUE
16.
150.
590.
230.
1600.
35.
0,88
1.5
1800.
22.
12.
0.25
0.27
2.5
2.3
1.3
3.8
0.89
5.9
6.2
0.86
3.2
4.4
20,
1.4
33.
< VALUE*UNIT OF DETECTION DETERMINED BY INSTRUMENT SENSTiSAHPLE DIL»  ANB MATRIX INTERFERENCE.
 RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
                                                                                                 EPA/RSKERL/ABAiOK

-------
                                                 ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
                                                           FOR:
                                                              LIST.LSTJ2233
  PROJECT:   OLD INSERS SITE SAMPLES
            COLLECTED  1/22/86
            w.i

  CONCENTRATION IN: M6/K6 DRY (IT.

 DATE        7MAY86       STDV
 TIME        10:            +/-
 TA6.NO,      1909           1909

 ELEMENT      VALUE          VALUE
7MAY86
10:0?
 1910


 VALUE
< VALUE*LIHIT OF DETECTION DETERMINED DY INSTRUMENT SENST»SAMPLE DIL» AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO 2 SIGNIFICANT DIBITS
STDV
 I/-
  VALUE
NA
K
CA
H6
FE
HN
CO
MO
AL
AS
SE
CO
DE
CU
CR
NI
ZN
AG
TL
PB
LI
SR
V
DA
B
TI
153.
1590,
4970.
2730.
15000.
260.
4.9
<1.
17300.
<20.
13.
<.3
0.86
7.9
22.3
13.4
37.4
<1.
<5.
12.7
9.2
25,0
32.5
168.
6.9
338.
15.
150.
490.
270.
1500.
25.
1.0
1.4
1700.
20.
11.*
0,30
0,31
2,3
2,2
1,4
4.0
1,0
5.4
5.5
1.0
2.4
4,0
16.
1,5
33.
147.
1500.
4670.
2740.
15500.
316.
5.67
<1.
17100.
<20.
12.
<,2
0.89
7.8
21.7
13.1
36.5
<,9
<5.
14.2
9.01
24.8
32,2
180.
5.9
304.
14.
ISO.
460.
270.
1500.
31.
0.97
1.4
1700.
20.
12.
0.28
0.29
2.4
2.1
1.3
3.9
0.98
5.3
5.5
0.95
2.4
4.0
18.
1.4
30.
140.
1110.
4210.
2250.
13600.
387,
5.78
<1.
14600.
<17.
12,
<.2
0.73
8.0
22.3
18.1
36,7
<,9
<4.
15.3
7.66
22.2
27,8
153.
7.0
268.
14.
110.
420.
220.
1300.
38.
0.94
1.2
1400.
17,
10,
0,27
0,28
2.1
2.2
1.8
3.9
0.94
4.6
5.0
0.93
2.2
3.4
15.
1.3
26.
164.
1340.
7840.
2380.
13700.
217,
4.56
<1.
16100.
<19.
11.
<.2
0.77
6.4
21,5
12.1
32.5
<.B
<5.
14,5
7.71
43.3
30.3
153.
6.1
302.
16.
130.
780.
230.
1300.
• 21.
0.82
1,3
1600.
19.
10.
0.24
0,25
2.1
2.1
1.2
3.5
0.82
5.2
5.3
0.80
4.3
3.7
15.
1.3
30.
                                                                                                  EPA/RSKERL/AOAfOK

-------
                                                ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
                                                          FOR!
                                                             LIST.LSTJ2261
  PROJECT:   OLD INGERS SITE T.A,
            P184.8

  CONCENTRATION IN: N6/K6 WET UT,
DATE
TIME
TAG.NO.


30DEC8S
14:01
56
1158
6ROUP2
STDV
f/-
56

PLOT 4
0-6* 10208
ELEMENT
NA
K
CA
H6
FE
MN
CO
NO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
VALUE
64.
1290.
4370.
2320.
13100.
257.
4.63
<1.
14800.
<17.
<10.
<.2
0.71
7.1
17.8
11.1
31.7
<.8
<4,
10.9
8.68
22.9
.29.4
163.
<1.
284.
VALUE
12.
130.
430.
230.
1300.
25.
0.84
1.2
1400.
17.
10.
0.24
0.26
2.0
1.7
1.1
3.4
0.84
4.4
4,9
0.88
2.2
3.6
16.
1.2
28.
                                        30DEC85
                                        14J02
                                         4436
                                         US?
                                         6ROUP2PLOT5
                                         0-6* 40209
STDV
  4456
                                         VALUE

                                          59]
                                         88?.
                                        6590.
                                        2110.
                                       12000.
                                         295.
                                           5.73
                                           1.2
                                       11700.
                                          0.59
                                          6.6
                                          15.7
                                          10.2
                                          30.6
                                          12.2
                                          6.91
                                          26.6
                                          24.2
                                         155.
                                          <1.
                                         196.
  VALUE

   12.
   90.
  650.
  210.
 1200.
   29.
    0.81
    1.0
 1100.
   14.
    9.5
    0.24
    0.24
    1.9
    1.5
    1.0
    3.3
    0.82
    3.8
    4.1
    0.80
    2.6
    3.0
   15.
    1.0
   19.
 30DEC85      STBV
 14.*04         */-
  336           336
  11S90UP 6RIND
  6ROUP2PLOT5
  0-6* 10209
  VALUE

   55.
 1030.
 3970.
 2090.
12900.
  302.
    5.35
    1.7
12900.
    0.66
    6.5
   16.7
   10.6
   30.7
   13.6
    6.98
   21.1
   28.1
  169.
   <1.
  223.
VALUE
             300EC85      STDV
             14:05         */-
              4366          4366
              11S9DUP 016 OF DUP 6RIND
              6ROUP 2 PLOT 5
              0-6f 10209
VALUE
11.
100.
390.
210.
1200.
29.
0.77
1.2
1200.
15.
10.
0.22
0.23
2.0
1.6
1.1
3.3
0.78
4.0
4.5
0.76
2.1
3.4
16.
1.0
22.
59,
1130.
4080.
2130.
13100.
280.
4.98
1.5
13600.
<16.
<10.
<,2
0.68
6.7
17.9
10.7
31.6
<.8
<4.
14.0
7.69
22.4
29.0
166.
<1.
249.
< VALUE«LINIT OF DETECTION DETERMINED BY INSTRUMENT SENSTtSANPLE DILt AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
 VALUE

  12.
 110.
 400.
 210.
1300.
  27.
   0.83
   1.2
1300.
  16.
  10.
   0.24
   0.25
   2.0
   1.7
   1.1
   3.4
   0.83
   4.2
   4.7
   0.81
   2.2
   3.5
  16.
   1.1
  25.
                                                                                                 EPA/RSKERL/AMiOK

-------
                                                ELEMENTAL CONSTITUENTS ANALYSIS BY  ICAP
                                                          FOR:
                                                             LIST.LSTI2261
 PROJECT:  out INGERS SITE T.A,
           P184.8

 CONCENTRATION IN! KG/KG MET HT.
DATE
TIME
TA6.NO.



ELEMENT
NA
K
CA
H6
FE
HN
CO
HO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
30DEC85
13Z54
626
1155
CONTROL
W205
VALUE
76.
1280.
4980.
2430.
12900.
299.
5.17
<1.
14800.
<17.
<10.
<.2
0.70
6.5
17.0
11,1
39.0
<.8
<4.
9.5
8.49
22.4
28.7
152.
<1,
267.
STDV
*/-
626

PLOT 0-6'

VALUE
13.
130.
490.
240.
1200.
29.
0.83
1.1
1400.
17.
10.
0.24
0.25
2.0
1,6
1.1
4.1
0.83
4.4
4.7
0.86
2.2
3.5
13.
1.7
26.
STDV
  2006
                                        30DEC8S
                                        13:56
                                         2006
                                         11S5DUP
                                         CONTROL PLOT 0-6'DUP
                                         M20S
                                         VALUE
                                        1250.
                                        5120.
                                        2410.
                                       13100.
                                         408.
                                           5.75
                                          <1.
                                       14700.
                                           0.71
                                           6,6
                                          16.4
                                          11.0
                                          33.6
                                          10.1
                                           8.40
                                          23.1
                                          29.4
                                         163.
                                          <1.
                                         275.
  VALUE

   13.
  120.
  510.
  240.
 1300.
   40.
    0.84
    1.1
 1400.
   17.
   10.
    0.24
    0.26
    2.0
    1.6
    1.1
    3.6
    0.84
    4.4
    4.7
    0.85
    2.3
    3.6
   16.
    1.2
   27.
 30DEC85
 13:58
  4306
  1156
  GROUP
  0-6*  *02M

  VALUE
  769.
 3760.
 1960.
12300.
  354.
    5.02
    <.9
11800.
    0.60
    6.2
   15.3
    9.8
   28.3
    9,9
    5.65
   18.2
   22.0
  154.
    <.9
  164.
< VALUE»LINIT OF DETECTION DETERMINED BY INSTRUMENT SENSTrSAHPLE DIL» AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
STDV
*/-
4306

NTROL PLOT2
6
VALUE
14.
78.
370.
190.
1200.
35.
0.78
0.97
1100.
14.
9.7
0.23
0.23
1.9
1.5
1,0
3.0
0.79
3.6
4.0
0.77
1.8
2.8
15.
0.99
16.
30DEC85
13:59
3056
1157
STDV
*/-
3056

6ROUP 1PLOT3
0-6' 10207
VALUE
101.
1390.
4100.
2200.
12500.
274.
4.80
<1.
14500.
<17.
<9,
<.2
0.71
6.6
16.5
10.6
28.7
<.8
<4.
9,6
8,57
22.1
28.9 .
148.
<1.
298.

VALUE
16.
130.
410.
220.
1200.
27.
0.81
1.1
1400.
17.
9,8
0,24
0.25
1.9
1.6
1.1
3.1
0.81
4.3
4.6
0.86
2.2
3.5
14.
1.2
29.
                                                                                                 EPA/RSKERL/ADAiOK

-------
                                                ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
                                                          FOR:
                                                            LIST.LSTJ2257

  PROJECT:  INGERS SITE SAMPLES COLLECTEB 9/20/85
           PI81,8

  CONCENTRATION IN5  M6/K6 MET HT

DATE        1MOV8S       STDV
TIME        14M2         */-
TA6.NO.      180R          180R

ELEMENT      VALUE         VALUE         VALUE        VALUE         VALUE         VALUE         VALUE        VALUE
MA
K
CA
N6
FE
m
CO
MO
AL
AS
SE
CD
BE
CU
CR
NI
»
AG
TL
PB
LI
SR
V
BA
B
TI
97,
1070.
4460.
2300.
13400.
444.
3,58
<1.
13800.
<17.
<10.
<.2
0.73
7.0
18,0
12.0
33.4
<,8
<4.
10.5
7.64
23.1
31,6
169.
3.1
281.
10.
100.
440.
230.
1300.
44.
0.83
1.1
1300,
17.
10.
0.24
0.25
2.1
1.7
1.1
3.6
0.83
4.4
4.5
0.81
2.3
3.8
16.
1.2
28.
< VALUE»LINIT OF DETECTION KTERHIND BY INSTRUMENT SENSTiSANPLE OILi AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO 2 SNNIFICANT BI6ITS
                                                                                                 EPA/RSKERL/AOAfOK

-------
                                                ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
                                                          FOR:
                                                             LIST.LSTJ2257
 PROJECT:   INGERS  SITE SAMPLES COLLECTED 9/20/93
           P181.8


 CONCENTRATION  IN!  H6/K6 UET  HT
DATE
TIME
TAB.NO.


ELEMENT
m
K
CA
N6
FE
MN
CO
MO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
* •••• *^_*
1NOV8S
14:05
186
PLOT 3
F1609
VALUE
103.
1110.
37W.
2210.
12200.
244.
4.05
<1.
13500.
<16.
 AND MATRIX  INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
                                                                                                  EPA/RSXERL/ADAiOK

-------
                                                ELEMENTAL  CONSTITUENTS ANALYSIS BY ICAP
                                                          FOR:    .
                                                            LIST.LSTJ2257
 PROJECT:  INGERS SITE SAMPLES COLLECTED 9/20/95
           P181.8

 CONCENTRATION IN!  H6/K6 MET VT
                                                                   1NOVB5
                                                                   14:02
                                                                    1MB
                                                                    GROUP 2 PLOT
                                                                    OHO
                                                                    VALUE

                                                                     93.
                                                                    897.
                                                                   4040.
                                                                   1910.
                                                                  11300.
                                                                    260.
                                                                      4.22
                                                                      5.8
                                                                  11600.
                                                                      0.41
                                                                      7,5
                                                                     18.1
                                                                     10.8
                                                                     38.3
                                                                     15.?
                                                                      6.74
                                                                     23.2
                                                                     25.0
                                                                    192.
                                                                      2.55
                                                                    177.
< VALUE*LIHIT OF DETECTION DETERMINE)  BY INSTRUMENT SENSTiSANPLE DILi AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
DATE
TINE
TA6.NO.


ELEMENT
NA
K
CA
H6
FE
MN
CO
HO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
AG
TL
PB
LI
SR
V
BA
B
TI
1NOV85
13:59
183
GROUP 2
0107
VALUE
94.
1010.
3700.
2030.
12100.
197.
3.95
2.2
13000.
<16.
<9.
<.2
0,60
6.6
17,5
10.1
42,2
<,7
<4,
12.6
7.19
20.9
28.7
156.
5.4
251.
STDV
*/-
183
PLOT 4 0-6'

VALUE
10.
100.
370.
200.
1200.
19.
0.79
1.2
1300.
16.
9.5
0,23
0,24
1,9
1.7
1.0
4.5
0.79
4,1
4.4
0,77
2.0
3.4
IS.
1.1
25.
1NOV85
14:
184
GROUP2
0110
VALUE
100.
1030.
3380.
1980.
11500.
181.
3.85
5.3
12600.
<15.
<9.
<,2
0,63
7.5
19.0
10,0
32.7
<.7
<3.
14.5
7.46
20.3
26.4
180.
4.5
233.
. &u ••Mk««UMM
STDV
t/-
184
PLOT 5 0-6'

VALUE
11.
100.
330.
190.
1100.
17.
0.78
1.5
1200.
IS.
9.1
0.23
0.23
1.8
1.8
1.0
3.5
0,78
3.9
4.5
0.76
2.0
3,2
18.
1.1
23.
•»•• 4^VUM9 M A MflM
STDV
*/-
1B4D
.OT 5 0-6'

DIGESnON
VALUE
10.
90.
400.
190.
1100.
25.
0,75
1.4
1100.
14.
8.9
0.22
0.22
1.7
1.7
1.0
4.1
0.76
3.6
4.3
0.74
2.3
3.0
19.
0.98
17.
1NOV85
14!03
185
PLOT 2 0-61
F1608

VALUE
102.
951.
3750.
2070.
12300.
229.
4.13
<1.
13100.
<16.
<9.
<.2
0.65
6,2
16.3
10.2
29,8
<,7
<4,
12,1
7.04
20.2
27.8
138.
3.0
261.
STDV
+/-
185



VALUE
11.
96.
370*
200.
1200.
22.
0.73
1.1
1300.
16.
9.6
0.21
0.22
1.9
1.6
1,0
3.2
0,73
4.0
4.4
0.71
2,0
3.3
13.
1.1
26.
                                                                                                 EPA/RSXERL/ADA»OK

-------
                                                ELEMENTAL CONSTITUENTS ANALYSIS BY  ICAP
                                                          FOR*
                                                             LIST.ISTI2257

 PROJECT:  INSERS SITE SAMPLES COLLECTED 9/20/85
           P181.8

 CONCENTRAnON IN:  MS/KB VET HT

BATE        1NOV83       STDV           1NOV85       STDV          1NOV8S       STDV           1NOV85       STDV
TINE        13544         f/-           13:45         */-          13J47        */-           13J49         */-
TA6.NO.      180           180           180B          180D         181            181           182           182
             PLOT 1 CONTROL 0-6'         PLOT 1 CONTROL 0-6'        GROUP  1  PLOT 2 0-6'         GROUP 1 PLOT 3 0-4*
             F1607                       F1607                      0101                       0104
                                         DUPLICATE DIGESTION

ELEMENT      VALUE         VALUE         VALUE         VALUE        VALUE          VALUE         VALUE         VALUE
NA
K
CA
N6
FE
MN
CO
NO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
108.
1180.
4390.
2310.
13400.
437.
S.63
<1.
14000.
<17.
<10.
<,2
0.78
7.1
18.1
12.0
33.0
<.8
<4.
10.4
8.17
23.1
30.9
170.
3.3
275.
11.
110.
430.
230.
1300.
43.
0.83
1.1
1400.
17.
10.
0.24
0.25
2.1
1.7
1.2
3.6
0.83
4.3
4.6
0.82
2.3
3.7
17.
1.2
27.
101.
1040.
3780.
2190.
13400.
327.
4.41
<1.
13400,
<16.
<10.
<.2
0.70
6,6
16,9
11.8
32.4
<.8
<4,
12.2
7.56
20.3
29.3
156.
6.7
265.
11.
100.
370.
220.
1300.
32.
0.82
1.1
1300.
16.
10.
0.24
0.25
2.1
1.6
1.1
3.5
0.82
4.3
4.6
0.80
2.0
3.5
IS.
1.2
26.
154.
1140.
3440.
2060.
12500.
298.
5.06
<1.
13800.
<17.
<9.
<,2
0.71
6.4
17.0
11.2
30.7
<.7
<4.
14.0
7.54
20.6
29.5
156.
5.0
300.
16.
110.
340.
200.
1200.
29.
0.79
1.1
1300.
17.
9.8
0.23
0.24
1,9
1.6
1.1
3.3
0.79
4.2
4.7
0.78
2.0
3.5
15.
1.2
30.
149,
954.
6260.
1980.
11800.
231.
4.14
<1.
12600.
<15.
<9.
<.2
0.64
6.6
16.4
9.89
29.5
<,8
<4.
10.2
6.95
26.5
25.9
135.
3.4
245.
15.
96.
620.
190.
1100.
22.
0.80
1.0
1200.
15.
9,3
0.23
0.24
1.8
1.6
0.98
3.2
0.80
4.1
4.1
0.79
2.6
3.1
13.
1.1
24.
< VALUE*LINIT OF DETECTION DETERMINED BY INSTRUMENT SENSTiSANPLE BILt AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
                                                                                                  EPA/RSKERL/ADA»OK

-------
                                                ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
                                                          FOR:
                                                             LIST.LSTI2263

 PROJECT:  IMSERS SITE SAMPLES-SUPERFUNB
           P174.8

 CONCENTRATION IN: MS/KB BET BEI6HT EXCEPT FOR 1550(SLUWE + LIQUID) IN N6/L

DATE      STDV          STDV          STDV          STDV          STDV          STDV          STDV        ' STDV
TIME       14:           V-           14U8        +/-          14:19         +/-           14:21         */-
TA6.IKJ.      308H          308H          4306          4306          516           516           4106          4106
             1550                        1550 DUPLICATE            1550 (LIQUID FROM SLUB6E)t$   1546 REBURN
             SLUD6E                      SLUB6E                    -RESULTS IN N8/L-

ELEKENT      VALUE         VALUE         VALUE         VALUE        VALUE         VALUE         VALUE         VALUE
MA
K
CA
H6
FE
MN
CO
MO
AL
AS
SE
CD
BE
CU
CR
Ml
IN
A6
TL
PB
LI
SR
V
BA
B
TI
126.
40.
227,
96,
1550,
12.0
3.6
28.9
541,
<3.
<3.
<«3
<.3
19.2
73.7
8.0
89.4
<1»
3.4
123.
<1.
38.8
11.}
886.
3.9
<11.
13.
29.
24.
11.
150.
1.1
1.1
2.9
54.
3.5
3.8
0.35
0.35
1.9
7.4
1.1
9.1
1.1
2.3
12.
1.1
3.8
1.2
88.
1.1
11.
118.
35.
232.
93.
1550.
12.1
3.4
27.2
497.
<3.
<3«
<»3
<«3
18.1
72.6
8.2
86.5
<1.
<2.
122.
<1.
37.3
11.4
844.
<1.
<10.
12.
27.
25.
10.
150.
1.1
1.0
2.7
SO.
3.3
3.5
0.32
0.32
1.8
7.2
1.0
8.8
1.0
2.2
12.
1.0
3.7
1.2
84.
1.1
10.
128.
5.27
65.3
35.9
233.
3.09
0.22
0.20
13.8
<•!
<»2
<»01
<.009
0.08
1.53
0.39
1.90
<.03
<.06
0.38
0.05
0.82
0.05
0.21
0.44
<»3
12.
0.82
6.5
3.6
23.
0.30
0.03
0.03
1.4
0.10
0.22
0.01
0.009
0.05
0.15
0.04
0.19
0.03
0.06
0,06
0.03
0.08
0.03
0.03
0.04
0.33
177.
3160.
4390.
4610.
19200.
246.
5.88
<1.
27600.
<25.
<17.
<»1
1.45
18.4
30.0
16.0
65.7
<.6
<7.
15.8
17.2
36.0
58.8
141.
20.3
223.
18.
310.
440.
460.
1900,
24.
0.66
1.9
2700.
25.
17.
0,19
0.20
3.7
2.9
1.6
6.9
0.64
7.0
5.8
1.7
3.6
6.7
14.
5.1
22.
< VALUE«LIMIT OF DETECTION  DETERMINED BY INSTRUMENT SENSTiSAMPLE DIL. AND MATRIX INTERFERENCE.
 RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
                                                                                                 EPA/RSKERL/ADAfOK


 »  SAMPLE COMPOSED OF TUO PHASES (SLUDGE AND LIQUID).   LIQUID MAS TAKEN FROM SURFACE OF SAMPLE AND ANALYZED SINCE
     THE SAMPLE COULD NOT BE SAMPLED REPRESENTATIVELY.  RESULTS REPORTED ABOVE AS N6/L OF LIQUID.

-------
                                                ELEMENTAL CONSTITUENTS ANALYSIS IY  ICAP
                                                          FOR:
                                                             LJST.LSTJ2263

 PROJECT:  INGERS SITE SAMPLES-SUPERFUND
           P174.8

 CONCENTRATION IN: M6/K6 VET HEIGHT EXCEPT FOR 1SSO(SIUD6E + LIQUID)  IN M6/L
                                       STDV          STDV
                                        13:55        +/-
                                         426           428
                                         1549 DUPLICATE
                                         BURIED HASTE
DATE
TINE
TA6.NO,


ELEMENT
NA
K
CA
H6
FE
HN
CO
MO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
Tl
PB
LI
SR
V
BA
B
' TI
STDV
13:53
4026
154?
BURIED
VALUE
487.
<50.
183.
69.
457.
5.3
<2,
<2.
189.
7,7
<6,
<,6
<.6
10.9
20.5
8.4
58.8
<2.
5.4
27.5
<2.
9.0
28.1
92.4
7,5
<20.
STDV
*/-
4026

HASTE
VALUE
50,
50.
21.
20.
46.
2.0
2.0
2.0
20.
6.0
6.0
0.60
0.60
2.0
2.1
2.0
6.1
2.0
4.0
4.0
2.0
2.0
2.8
9.2
2,0
20.
                                        VALUE

                                        543,
                                        <46.
                                        194.
                                          73.
                                        472.
                                           5.2
                                         216.
                                           7.5
                                          12.1
                                          19,8
                                           9.2
                                          67.4
                                          
-------
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-------
                                                     SHEET NO.
CHAIN OF CUSTODY  RECORD
                                      -A
Mnjrrr MAMr^ Ji
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^
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COMMENTS
////V//7^f
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> ncrr/TMF
nATT/TIMF
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BATT/TIMr

-------
                                                                                                              SHEET NO..
PROJECT NAME

PROJECT no
                                CHAIN  OF CUSTODY  RECORD


                               	    SAMPLER (S) SIGNATUR
                                                                                                       
-------

-------

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-------
                                                                                                                       SHEET NO _ie±l OF
 PROJECT NAMC

 PROJECT NO.
                                  CHAIN  OF CUSTODY  RECORD


                                 	     SAMPLER (SI SIGNATURE
                                                                                            ^
     SAMPLE
  IDENTIFICATION
SAMPLING
LOCATION
 DATE
SAMPLED
                                            SAMPLE TYPE
                                                10 AM  OIL
  VOLUME
  TO BE
COLLECTED
  NO OF
CONTAINERS
   TIME
COLLECTIO
   BEGAN
   TIME
COLLECTION
COMPLETED
                                                                                                                        COMMENTS
                                                                 LpL
                                                                                                   JUX
                                                          X
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AUTHORIZATION FOR DISPOSAL.
                               m
                      , DATE/TIME

                      . DATE/TIME

                      DATE/TIME
                            !^££Jfl>VE(xivED BY NAME"

                            _     RECEIVED BY: NAME .

                            _     RECEIVED BY: NAME

                            _     DISPOSED BY: 	
                                                   DATE /TIME.

                                                   DATE/TIME
                                                                                                                    HATF/TIMF

-------
 PROJECT

 PROJECT NO.
                                 CHAIN  OF CUSTODY RECORD


                                	     SAMPLER(S)  SIGNATUR
                                                                                                                    SHEET NO
                                                             b/V/S1^  £   VvS/ffK
     SAMPLE
  IDENTIFICATION
SAMPLING
LOCATION
 DATE
SAMPLED
                                           SAMPLE TYPE
                                               10 AW  OIL
  VOLUME
  TO BE
COLLECTED
  NttOF
CONTAINERS
   TIME
COLLECTION
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COLLECTION
COMPLETED
                                                                                                                     COMMENTS
               £>*vz/ay/
                                                           X"
                                                                /
                                                                           L
RELINQUISHED BY: NAME	
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AUTHORIZATION FOR DISPOSAL.
                     .DATE/TIME

                      DATE/TIME

                     . DATE/TIME

                      DATE/TIME
                                                    7/z3
                                         BY^ NAME.

                                 RECEIVED BY: NAME.

                                 RECEIVED BY: NAME .

                                 DISPOSED BY: 	
                                                  DATE/TIME-

                                                  DATE/TIME .

                                                  DATE/TIME.

                                                  DATE/TMC.

-------
 EEJ
 PROJECT NAME

 PROJECT M
                                                                                        SHEET NO..
                                                                                                  .OF
                              CHAIN OF CUSTODY RECORD


                              	     SAMPLER (S)  SIGNATUR
    SAMPLE
  IDENTIFICATION
SAMPLING
LOCATION
 DATE
SAMPLED
                                       SAMPLE TYPE
                                           10 AIM  OIL
 VOLUME
  TO BE
COLLECTED
  NO. OF
CONTAINERS
  TIME
COLLECTION
  BEGAN
   TIME
COLLECTION
COMPLETED
COMMENTS
                   f /
                                                                                                   '1'
                                                                                   12^1
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                                     DATE/TIME-

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                                     DATE/TIME.

-------
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PROJECT NAME.

PROJECT NO	
                          Lna-fS''
                                                    CHAIN OF CUSTODY RECORD


                                                   	     SAMPLER(S) SIGNATURE
    SAMPLE
  IDENTIFICATION
                   SAMPLING
                   LOCATION
 DATE
SAMPLED
                                          SAMPLE TYPE
                                              io *m OIL
 VOLUME
  TO BE
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  NO. OF
CONTAINERS
   TIME
COLLECTION
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COLLECTION
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COMMENTS
                                                                                   ''&>
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                                                                                                                DATE/TIME.

                                                                                                                DATE/TIME.

                                                                                                                DATE/TIME.

                                                                                                                DATE/TIME.

-------