EPA/540/2-89/004
SUPERFUNDTREATABILITY
CLEARINGHOUSE
Document Reference:
Portier R., et al. "Field Plot Test Report, Phase III Engineering Design, Old Inger
Superfund Site, Darrow, Louisiana." Approximately 250 pp. November 1986.
EPA LIBRARY NUMBER:
Superfund Treatability Clearinghouse - EUQX
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SUPERFUND TREATABILITT CLEARINGHOUSE ABSTRACT
Treatment Process:
Media:
Document Reference:
Document Type:
Contact:
Site Name:
Location of Test:
Biological - Composting/Biodegradation
Soil/Sandy
Portier R., et al. "Field Plot Test Report, Phase
III Engineering Design, Old Inger Superfund Site,
Darrow, Louisiana." Approximately 250 pp.
November 1986.
Contractor/Vendor Treatability Study
Timothy Mahon
U.S. EPA - Region VI
1445 Ross Avenue
12th Floor, Suite 1200
Dallas, TX 75202
214-655-6444
Old Inger Site, LA (NPL)
Ascension Parish, LA
BACKGROUND; This project report describes the results of biodegradation
with indigenous microorganisms on soils at an oil reclamation plant. The
site occupied about 16 acres including a 7.5 acre swamp. The wastes were
oily sludges found in lagoons, diked tank containment areas, buried waste
areas and in the swamp. Wastes identified at the site were consistent with
hazardous materials used at an oil reclamation plant. Benzene, toluene and
PAHs were present; no PCBs were found and very low levels of chlorinated
hydrocarbons and heavy metals were detected. Numerous PAHs such as
naphthalene, methyl naphthalene, anthracene and fluorene were detected in
lagoon soils and buried waste soils. The concentrations of PAH compounds
ranged from less than 100 ppm to approximately 5700 ppm for phenanthrene.
OPERATIONAL INFORMATION; The purpose of the study was to determine
microorganism loading rate on the silt and sandy clay soils. Task I was a
screening test to determine the maximum toxicant loading rates. After
selection of the loading rate, Task II was mesocosm tests in the laboratory
where loading, nutrients and other parameters could be controlled. This
included evaluation of commercially available bacterial cultures. Field
verification studies (Task III) were conducted on special plots set off at
the site and the plots were loaded sequentially with different waste types.
The volume of soil which was treated was not reported. The duration of the
treatment was 35 days. The report contains a discussion of the mechanism
of biodegradation and an appendix showing the actual chemical reaction
pathways associated with the biodegradation of various PAH compounds.
PERFORMANCE; Optimal loading rates of the various contaminants were shown
to induce microbial biotransformations. All of the compounds studied
decreased in concentration over time, but no specific correlations were
presented or discussed by the authors. Data that was generated only
3/89-11 Document Number: EUQX
NOTE: Quality assurance of data may not be appropriate for all uses.
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indicated gross trends and no contaminant destruction efficiencies were
reported. Also there was no analysis for toxic intermediates in this study
and the authors suggested that toxic intermediate production needed to be
evaluated further. No specific QA/QC procedures were reported. The
authors state that microbial degradation and detoxification of the site is
scientifically verifiable and economically feasible although no discussion
of the economics was contained in the study. Post closure monitoring of
soils and leachate from the site was recommended for 30 years.
CONTAMINANTS:
Analytical data is provided in the treatability study report. The
breakdown of the contaminants by treatability group is:
Treatability Group CAS Number Contaminants
W08-Polynuclear Aromatic 120-12-7 Anthracene
91-20-3 Naphthalene
85-01-8 Phenanthrene
208-96-8 Acenaphthylene
86-73-7 Fluorene
206-44-0 Fluoranthene
3/89-11 Document Number: EUQX
NOTE: Quality assurance of data may not be appropriate for all uses.
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- 7 >'~J<1- i
November 18, 1986
Mr. William B. DeVille DWMS Project No. 6752-0101
Administrator GDC Project No. 85-501
Inactive and Abandoned Sites Division DEQ Contract No. 25202-85-01
Louisiana Department of Correspondence No. 84
Environmental Quality
P. 0. Box 44307
Baton Rouge, LA 70804
Transmittal
Field Plot Test Report
Old Inger Superfund Site
Parrow. Louisiana
Dear Mr. DeVille:
IT Corporation is herein transmitting the report of the field plot tests
as prepared by LSU. LSU was subcontracted by IT to review data from the
field tests and other tests performed by West Paine Laboratories and
Robert S. Ke,rr Environmental Research Laboratory. The review of the
data was to substantiate other facts that indicated biodegradation as
the most feasible and environmentally sound remedial action alternative
at the site.
In summary, LSU made positive input in public attitudes about
biodegradation. The reports concludes that this alternative will work
if properly managed. IT/GDC did not wish to rewrite the report in a
more amiable (as far as IT/GDC is concerned) format, however LSU's
conclusions are supported and favorable to the overall project.
Yours Truly,
Mark L. Morgan, P. E.
Senior Project Manager
MLM:jlc
Attachment
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K
Project Report: Old Inger Abandoned Hazardous Waste Site,
Darrow, Louisiana
In Vitro and In Situ Investigations
La DEQ Contract No. 25202-85-01
IT. Project No. 435062
Submitted to:
Mr. Mark Morgan
IT Corporation, Baton Rouge, LA
Submitted by:
Ralph Portier, Ph.D.
Martina Bianchini, M.S
Paul Templet, Ph.D.
Debra McMillin, M.S.
Charles Henry, B.Sc.
Institute for Environmental Studies,
Louisiana State University, Baton Rouge, LA
I.E.S. Technical Report Number: 86-09-20-6103
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ACKNOWLEDGEMENTS
Mrs Susan Cange and Ms Debra Saxton, project engineers of IT Corporation and
GDC Inc., respectively, coordinated the collection and transportation of the field samples
to our laboratory. It must be acknowledged, that IT Corporation as prime contractor of this
project was responsible for the preparation, application and subsequent sampling in all
field studies and related activities conducted. Their contribution is greatly appreciated.
Mr. John Matthews, research biologist at US- EPA Robert S. Kerr
Environmental Research Laboratory, Ada, OK provided Microtox™ support and
Microtox™ data sets. His interpretation of these data sets and his input into microbiological
investigations is recognized and appreciated.
This project was supported by funds from LSU contract # 135-20- 6103 with
funds provided by IT Corporation through a general technical service contract with the
Louisiana Department of Environmental Quality (DEQ) (Hazardous and Abandoned Waste
Sites, Mr. William Deville, Director). IT Project No. 435062. DEQ Contract 25202-85-01
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TABLE OF CONTENTS
PAGE
List of Figures vi
List of Tables v
I. Introduction 1
n. Site Characterization 5
1. Location 5
2. History 2
3. Waste Identification 2
4. Site Soil Profile 2
HI. Materials and Methods 6
A. Experimental Design 6
1. General 6
2. Screening Tests '...,. 6
3. Mesocosm Tests 8
a. Addition of Commercial Inoculum 8
b. Sequential Re-loading of 4% and 2.5% Waste Mesocosms 9
4. Field Verification Studies 9
B. Field Sample Collection 11
C. Biological Methods 11
1. Microbial Diversity 11
2. Microbial Adenosine 5* Triphosphate (ATP) 12
3. Microtox™ 12
4. Plant Biomass Determination 13
D. Analytical Methods 15
1. Soil/Waste Chromatographic Analysis 15
2. High Performance Liquid Chromatography (HPLC) 15
3. Soil Moisture and pH 16
IV. Results 17
A. Pure Waste Analysis 17
B. Screening Tests 17
in
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TABLE OF CONTENTS (CONTD)
C Mesocosm Tests 24
l.Chromatographic Analysis 24
2. Microbial ATP 29
3. Microbial Diversity 29
4. Microtox™ 34
5. Commercial Inoculum Test 36
6. Sequential Re-loading of 4% and 2.5 % Waste Mesocosms 40
7. High Performance Liquid Chromatography (HPLC) 40
8. Soil Moisture and pH 41
D. Field Verification Studies 46
1. Chromatographic Analysis 46
2. Microbial ATP 52
3. Microbial Diversity 57
4. Microtox™ and TOC Analysis '. 61
5. Soil Moisture and pEL 63
6. Plant Biomass Determination 68
V. Discussion 71
VI. Conclusions and Recommendations 78
VH. Bibliography 81
VIE. Appendices 87
ill
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LIST OF FIGURES
FIGURE TITLE PAGE
1 Chemical structures of some common aromatic hydrocarbons 3
2 Experimental design 7
3 Mesocosm identification
4 Microbial ATP profile of screening test mesocosms 21
5 Microbial diversity of screening test mesocosms 22
6 Microtox™ analysis of WSF of screening test mesocosm soil/waste loading 23
7 GC/FBD analysis for 4% lagoon waste, F-2 fraction over a 35 day time frame 25
8 GC/FID analysis for 4% lagoon waste, F-l fraction over a 35 day time frame 26
9 GC/FID analysis for 2.5% lagoon waste, F-2 fraction over a 35 day time frame 21
10 Microbial ATP profiles of 4% lagoon mesocosms plus Cl, day 7-35 30
11 Microbial ATP profiles of 2% lagoon mesocosms plus Cl, day 2-35 31
12 Microbial diversity of mesocosms plus Cl, day 7-35 32
13 Microbial diversity of mesocosms day 28-84 33
14 Microtox™ toxicity results of mesocosms 35
15 Total ion chromatograms of mesocosms with commercial inoculum. 37
16 Degradation rates for commercial inoculum as compared to 4% adapted inoculum,.. 38
17 Degradation rates for commercial inoculum as compared to 2.5% adapted inoculum. 39
18 GC/FID analysis for group I and group n over a 25 day time frame 47
19 GC/FID analysis for group I, F-2 fractions, day 65-156 48
20 GC/FID analysis for group n, F-2 fractions, day 46-156 49
21 GC/FID analysis for PL50"-20" 51
22 GC/FID analysis for alternate locations of PL5,0"-6" 53
23 Microbial ATP profiles of field plots, day 0-37 54
24 Microbial ATP profiles of field plots, day 51-113 55
25 Microbial ATP profiles of field plots, day 156-184 56
26 Microbial diversity of field plots, day 23-58 58
27 Microbial diversity of field plots, day 102-141 59
28 Microbial diversity of field plots, day 170-184 60
29 Microtox™ toxicity results of field samples, 0"-6" 62
30 TOC analysis for day 149 .64
31 TOC analysis for day 184 .65
32 Plant biomass determination of field plots 69
IV
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LIST OF TABLES
TABLE TITLE PAGE
1 Schedule of field plot activities 16.
2 Waste statistics 24
3 Primary F-2 constituents in CERCLA waste site 26
4 Primary F-l constituents in CERCLA waste site 27
5 Total Organic (TOC) analysis for laboratory mesocosms 28
6 F-2 fraction; 2.5% lagoon waste 36
7 % Moisture of laboratory mesocosms 50
8 pH Profiles of laboratory mesocosms 52
9 % Moisture of fieldplots 75
10 pH Profiles of field plots 76
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/. Introduction
This study reports the results of a multi task project for achieving biological closure
of an abandoned hazardous waste site situated on the Mississippi River. Biological closure
is a method which employs microorganisms to detoxify chemical compounds present in
hazardous waste sites.
The study was divided into three tasks, namely task I (screening tests), task It
(mesocosm tests) and task HI (field verification studies). In all three tasks waste materials
from the site were evaluated for biodegradative potential and acute toxicity of leachate. The
validity of the task I tests for establishing biotransformation/biodegradation kinetics and
leachate toxicity were documented in subsequent task n laboratory mesocosm studies and
task HI field studies.
The selected cleanup procedure for the site was onsite biodegradatipnjiirougjhland
treatment orjn situ biological treatment (ISBT). This method was chosen because it is
consistent with the National Contingency Plan in that it provides the lowest cost alternative
while providing a permanent remedy for an existing or potential threat to public health and
the environment Onsite biodegradation through land treatment (ISBT) was selected as the
least expensive alternative and most applicable to the site. This technique consists of
spreading and mixing the oily sludges over land^providing nutrient supplements,
optimizing the necessary growth conditions for the microbial growth, and promoting the
decomposition of hydrocarbon components by the indigenous microbiota (8). Such
manipulation of the site, "while favoring microbial growth and promoting the degradation
of the toxicants, also reduces the potential threat of ground water pollution. A literature
review explaining microbial hydrocarbon degradation processes is presented in Appendix
A.
According to RCRA regulations, after successful completion of task I, n and ffl
studies, soils and wastewater must still be biologically monitored to prepare the site for the
final remediation consisting of the establishment of a permanent vegetative cover and post-
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Louisiana Environmental Control Commission in June, 1981, that the site was
abandoned. Appendix A shows a map of the site and the nature of the contaminated
areas. The site occupies about sixteen acres, including a 7.5 acre swamp. The wastes are
found in lagoons I and n, the diked tank containment area, the buried waste area and the
swamp. Contamination is observed to a depth of three to five feet in the areas of the
lagoon I and filled portion of the swamp. Swamp sediments are less contaminated.
Lagoon I occupies an area of 0.7 acre. At least 50 partially filled 55 gallon waste drums
are located in the northeastern comer of that lagoon and in the filled southeastern comer
of the swamp (17).
3. Waste Identification
The wastes identified at the site were consistent with the nature of the oil
reclamation plant They were mixtures of refinery oils, motor oils, and lubricating o'ils. As
is typical of waste oils, hazardous priority pollutants such asJbenzene, toluene and PAHs
were present (16). No PCB's were found; very low levels of chlorinated hydrocarbons and
low levels of heavy metals were found. The waste statistics are shown in Table 2.
4. Site Soil Profile
The site soil consists predominantly of silty and sandy clays, silts and fine sands, to a
depth of about 115 to 125 feet (16). Below this is a substratum silty sand, a potential water
supply source. The average vertical and horizontal permeability were both about Ix 10~5
cm/ sec (10 ft/ year). Groundwater was encountered generally ata depth of six to twelve
feet, however rising to within a few feet of the ground surface (16). Trace amounts of
some hazardous compounds had migrated vertically through the site soils to depths of 20
feet or more. Trace amounts (parts per billion) were found in the groundwater at the site to
a depth of 75 feet (17). The potential for continued vertical and horizontal migration of
hazardous compounds exists.
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FIG. 1: Chemical structures of some common aromatic hydrocarbons.
Alkyl Benzene
Naphthalene
Anthracene
Acenapthene
Phenanthrene
Pyrene
Chiysene
Benzo[a]pyrene
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TABLE 2:
Waste Statistics
Type
Maior Problem Area
Jiuantitv
Oily sludges
Heavily cont. soil
slightly cont. soil
Heavily cont. fluid
slightly cont water
cont. wood
slightly conL
groundwater
Total:
waste oils, sludges and
heavily (visibly) contaminated
soils and sediments
Lagoon 1
Lagoon 1
Buried waste area
swamp, S.E. swamp
all areas
Lagoon 1
tanks
swamp
swamp
shallow silt lens
7,600 CY
34,ooo CY
70,000 CY
2.5 million gallons
7.5 million gallons
5000 tons
10 million gallons
51.500CY
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closure monitoring. The cleanup time required using this method (ISBT) takes the longest
time of all alternatives, but provides a permanent solution to this public health and
environmental threat. Risks would be posed by using other techniques. For example,
offsite RCRA landfill would not only pose a transportation problem, but just place the
sludges in long term storage, where they could pose a potential threat and demand future
remedial actions.
II. Site Chracterization
1. Location
The hazardous waste site investigated was the "Old Inger" site. This is a CERCLA
site (Comprehensive Environmental Response, Compensation and Liability Act of 1980)
located on die East bank of the Mississippi River. The site lies about 4.5 miles north of
Darrow, LA, in Ascension Parish between Highway 75 and the Mississippi River. In April
1982, the site was designated as Louisiana's number one priority forSuperfund
assistance. It scored highest out of the five qualifying sites in Louisiana, with 48.98 on the
EPA Superfund list
2. History
Old Inger is an abandoned oil reclamation facility that was operated between
1967 and 1978. During these times, waste oils were brought to the site by barge and
truck. The oil was re-processed in the larger of the two cracking towers by heating the
heavier oils to increase their viscosity and mixing them with used lubrication oils, light
oils and spent solvents (16). Finalproducts were transported from the facility by truck.
As part of the plant operation, sludges were stored in a large, open lagoon and/or buried
in a shallow pit; some wastes were spilled into the adjacent swamp. In March 1978, a
large spill contaminated a total of 16 acres of the surrounding area. This spill was
associated with the unloading of used oil from a barge in the Mississippi River. A shut
off valve failure or human error led to overtopping a tank and containment area (16).
Failure by the owner to clean up the site resulted in the formal declaration by the
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///. Materials and Methods
A. Experimental Design
1. General
The study was comprised of three tasks, namely task I, task n, and task EL
Task I consisted of a series of screening tests with the objective of determining the
maximum toxicant loading rate that would not stress the microorganisms. These
screening tests involved the determination of optimal toxicant loading rates for the
CERCLA site on the basis of both indigenous microbial population performance and
acute toxicity by leachate analysis. The major correlative aspects of task I,n and ffl, are
depicted in Fig.2.
2. Sreening Tests
Screening tests consisted of a series of experimental mesocosms with a mixture
of Mississippi river silt and top soil (sandy clay). Soil mixtures type I and type n
consisted of one part river silt and two parts top soil, and two parts river silt and one
part top soil, respectively. The soils were collected from Johnson's Pit located
approximately 11 miles from the site and air dried prior to analyzation. The soils were
passed through a no. 10 sieve (Soiltest, Inc.) to remove larger particles and plant
residues, and also to render them more homogenous. Soil type n was found to be
unsuitable because of its lower £€59, detected by EPA research laboratory in Ada, OK.
The EC 50 was defined as the Vol % distilled, deionized water (DW) extract effecting
50% decrease in bacterial bioluminescence during a 5 min test period. It represented a
relative measure of acute toxic characteristics of water soluble constituents contained in
the soil/waste matrix.Soil type I, therefore, was selected for the course of the
experiment The loading rates of the wastes mixed with soil type I were 2% oil and
grease (O&G), 4% O&G, 8% O&G and 12% O&G by weight, based on standard oil
and grease test) for both lagoon and buried wastes. No nutrient amendment was added.
The microbial activity was evaluated 2 hours after mixing, and re-evaluated over a
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FIG. 2
Experimental design
SREENING TEST
LITERATURE
SUBYEY
COMPUTER
BASES
FATE/CFFECT KINETICS
TRANSPORT STUDIES
MESOCOSM TEST
M1CRQBIAL
ATP
SOIL:::::::
ENZYMOLOGY
FATE: ::::
ANALYSIS
MICROB-JAL
DIYCRSITY
BltDACCUM
ULATIOM '•
:POTE:NTJAIS
: HEAVY ::::
METALS
KINETIC MODELS
LEACHATE/
LOADING
RATE
RATIOS
SOIL/
WATER
SATURATION
EXPERIMENTS
SURFACE
RUNOFF
TESTS
MIGRATION
MODELS
9D
O
TOXICOLOGY/NET DETOXIFICATION ASSESSMENT
BASE
^^^^
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period of 17 days after mixing. The optimal loading rates for each waste type were
indicated by the highest levels of microbial activity.
3. Mesocosm Tests
After determination and selection of an acceptable loading rate, task n studies were
carried out The objective was to evaluate microbial biotransformation and biodegradation
of key pollutants in the laboratory, under controlled parameters, such as pH and moisture,
and to determine the optimal re-application schedule for the subsequent field verification
studies. A battery of 21 mesocosms was set up in the laboratory to evaluate
biotransformation processes under the selected loading rates. All mesocosms were set up in
triplicate. CS1, CS2 and CS3 were controls without toxicant loadings. 1L4,2L4 and 3L4
were test replicates, loaded with 4% lagoon waste. 1B4,2B4 and 3B4', also test replicates,
were loaded with 4% buried waste. 1L2, 2L2 and 3L2; 1B2,2B2 and 3B2 were test
replicates loaded with 2.5 % waste for lagoon and buried waste, respectively. Mesocosm
identifications with the soil/ waste concentrations are given in Fig.3. Along with the waste
loadings, each mesocosm (4.5 kg soil mixture type I) received nutrient amendments
consisting of 4.0 g KH2?O4) 2.0 g K2HPO4,1.0 g MgSO4 and 0.5 g KNO3. Nutrient
amendments were incorporated very thoroughly into the soil mixture, together with the
waste application, at day zero. A second nutrient amendment consisting of 2.0 g KH2PC>4,
2.0 g K2HPO4,1.0 g MgSC>4 and 0 J g KNO3, followed on day 27. The moisture was
also adjusted on day 27. Since microbial oxidation of PAH requires molecular oxygen, the
whole contents of the mesocosms were re-mixed weekly, to guarantee oxygenated
conditions.
a. Addition ofCommerial Inoculum
In addition to investigations of the biotransformation processes by indigenous
microflora, it was of special interest to evaluate the use of a commercially available blend of
bacterial cultures. These commercial cultures are marketed for their known ability to
degrade polynuclear aromatics. Their application is referred to by the supplier as
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"bioaugmentation". The inoculum used in the experiment was purchased from Microbe
Masters, Inc., Baton Rouge, LA. For further product data and application instructions, see
Appendix B. Two of the 21 mesocosms were inoculated with the commercial bacterial
blend at the suggested rate of 0.01 lb/ 4.5 kg soil mixture and labelled Cl and C2. Cl
contained lagoon waste at 4% load plus the inoculum. C2 mesocosm contained buried
waste at 4% load plus the inoculum. These mesocosms were inoculated at day zero.
b. Sequential Re-loading of 4% and 2 5% Waste Mesocosms
Another aspect of the design consisted of sequential reloading the 4% and 2.5 %
mesocosms after the initial wastes had been sustantially degraded The study of the effects
of repeated waste applications would allow extrapolations for the task IH field verification
studies. At day 35, the mesocosms labeled 1L4,2L4,3L4,1L2,2L2,3L2,
1B4,2B4,3B4,1B2,2B2 and 3B2 were reloaded with the same waste concentrations as
initially received.
4. Field Verification Studies
In task m, the field verification studies, 5 plots of 12' x 50' x2' were set off at the
site, filled with soil type I and loaded sequentially with the two waste types. Plot one
(PL1) served as a control plot (no toxicant loading). Plots two and three (PL2, PL3) were
classified as group I and received only buried waste loadings. Plots four and five (PL4,
PL5) were classified as group n and received alternate waste applications of buried and
lagoon waste at loading rates of 4% and 2.5% O & G. Periodic tillage and fertilization of
all the plots (control included) were carried out to optimize the biodegradation. The
objectives of the field plot activities were to: 1) determine the fate of the waste in the
ecological system: 2) to determine whether it was degraded, immobilized or transformed;
and 3) whether hazardous constituents were migrating below the treatment zone. A
complete schedule of the field plot activities is given in Table 1. All field applications and
collections were performed by and/or under the supervision of the prime contractor on this
project, IT Corporation. They were started in Oct. 1986 and completed in April 1986.
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TABLE 1:
SCHEDULE OF FIELD PLOT ACTIVITIES
DAY
0
14
23
46
51
59
102
DATE
10/08/85
10/09/85
10/10/85
10/25/85
11/04/85
11/25/85
(day 30)
12/02/85
12/09/85
01/22/86
(day 60)
WASTE APPLICATION
4% Buried (129 gaUplot or 2.3 drums/plot)
4% lagoon (339 galVplot or 6 drums/plot)
2% lagoon (170 gaUplot or 3 drums/plot)
2.5% buried (80.49 gal./plot or 1.5 drums/plot)
PLOT
PL2, PL3
PL4.PL5
PL4.PL5
PL2, PL3
PL4, PL5
ACTIVITIES
Tilling of PL4.PL5
Fertilization (5 Ibs/plot
10-5-5) and tilling of all f
plots
Raking of surface and
removing of crust of all p
156
03/18/86
2.5% buried (1.5 drums/plot) PL4, PL5
Tilling of PL4.PL5
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B. Field Sample Collection
Soil samples and soil cores were collected at random on each plot with a Shelby
tube sampler. Six random soil samples were composited to form one sample. Records
were kept to to make sure the same areas were not resampled. Holes were plugged with
fresh soil. Microbial samples were taken once a week to a depth of six inches for general
biological monitoring. The soil core samples were taken after the 2nd and 4th waste
loading, from a depth of 0-24 inches and analyzed in 6-inch increments. Sampling days
were field adjusted according to the soil moisture contents. No samples were taken after
heavy rainfalls. All the microbiological methods were performed on fresh soil samples
(stored on ice dun" - transportation to the laboratory). Analysis was within 3 h after
sampling.
C. Biological Methods
The following standard microbial activity estimations, bioassays and analytical
methods were conducted for all tasks of the research project:
1. Microbial Diversity
The Standard Plate Count Method (SPC) is a direct quantitative measurement of the
viable aerobic and facultative anaeobic microflora. Four general groups of
microorganisms, i.e. bacteria, actinomycetes, yeasts and filamentous fungi were
enumerated using colony forming units (CPU) and SPC. 1.0 g of soil (wet weight) was
weighed into 99 ml sterile, distilled and deionized water. Further dilutions were made in
phosphate buffer, as described in the EPA Manual 600/8-78-017.(20). For the
enumeration of bacteria and actinomycetes, replicate 1.0-ml aliquots were inoculated on
Jensens agar medium (31) supplemented with 40 Jig/ml cycloheximidine (Sigma) to inhibit
growth of filamentous fungi. All plates were incubated for 4 d at 30 C. Bacteria and
actinomycetes were counted on an automatic colony counter (Biotran m, New Brunswick
Scientific).The spot size of the monitor was set on 0.2 mm throughout all measurements.
Filamentous fungi and yeasts were enumerated on Martins agar medium (41), with 30
11
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jig/ml streptomycin (Sigma) and 30|il/ml chlorotetracycline (Sigma) to retard bacterial
growth. All plates were incubated for 4 d at 30 C, and were enumerated manually.
2. Adenosine 5'Triphosphate (ATP)
A modification of the adenosine 5' triphosphate (ATP) assay as advanced by
Holm-Hansen and Booth (28), and further presented by Van de Werf .Verstraete (62) and
Karl (34), was used for determination of microbial biomass. 1.0 g aliquots from all
mesocosms (wet weight) were transferred into dilution bottles containing 99 ml sterile
distilled and deionized water, homogenized on a homogenizer (Janke&Kunkel Ultra Turrax
SD 45) for 45 sec. A 100 pi aliquot of that suspension was then transferred to a 3 ml
plastic vial. The vial was inserted into a Lumac 3M Biocounter (Biocounter M 2010,
Lumac Systems, USA), 100 pi of buffer and 100 pi Nucleotide releasing agent for
bacterial cells (NRB reagent) were added. ATP was released from microbial cells by
adding NRB to a 100 jil sample. After application of this reagent, ATP was measured
using the following reactions:
Luciferin + Luciferase +ATP -Mg++_> (Luciferin-Luciferase-AMP) + ppj
(Luciferin-Luciferase-AMP)-O2-> Decarboxyluciferin +Luciferase +CO2+ AMP + light
Following the injection of 100 pi of a Luciferin luciferase solution (Lumit, Lumac
systems) into the vial, light outputs expressed as relative light units (RLU) were
determined over a 10 sec integration period. Relative light units were expressed as
|ig ATP/g dry net weight using standardized 10 pi aliquots of a known ATP standard.
Quench corrections for each environmental sample were established with 100 |il aliquots of
ATP standard /buffer added in place of the 100 pi buffer addition.
3. Microtox™
Acute toxicity assessment of waste/soil water soluble fractions (WSF) was
determined following procedures described in the EPA Permit Guidance Manual on
Hazardous Waste Land Treatment Demonstrations (19). A maximum acceptable initial
loading rate for task I studies was determined by calculating the EC50 and 95% confidence
12
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intervals for the raw waste WSF. The lower EC5Q limit for the confidence interval provides
the maximum loading rate for waste/ soil toxicity testing. In this case, the EC 50 is the
volume % of the distilled deionized water (DW) extract effecting a 50% decrease in
bioluminescence of the luminescent marine bacterium, Photobacterium phosphoreum
during a 5 min test period. The DW extract was obtained by shaking 400 ml DW + 100 g
sample for 24 h.
Mesocosm and field analyses are reported using LL5 values, defined as the five
minute mean light loss for the combined full strength (100%) and half strength (50%)
dilutions of the DW extract. T.T.5 values combine the measured dose-response with the
slope of the dose-response curve to provide a relative measure of the acute impact of the
WSF of the waste-soil matrix for comparative purposes when a quantitative EC50 cannot be
established. Results are reported +- relative standard deviation calculated for all test results
falling within a given LL5 range. A toxic effect is observed (OTE) when definite loss of
light is more than 20% (LL5 > 20%). LL5 values of 20-25% are considered to be in the
transition area and must be interpreted with care (Matthews, personnel communication).
Stimulation of bioluminescence was reported where TI.5 values were £ -5%. All samples
were analyzed on a Microtox model 2055 Toxicity Analyzer (Beckman Instruments, Inc.,
Carlsbad,CA). All reagents and test bacteria were supplied by the manufacturer. Microtox
analysis for this project were conducted by EPA personnel at the Kerr McGee Laboratories,
ADA.OK.
4. Plant Biomass Determination
Four months after the last activities at the field plots were carried out, a sampling of
the above ground plant biomass of the individual plots was sampled. The amount of plant
biomass was indicative of phytotoxic residual levels of oil sludges incorporated into the soil
earlier. From each plot, within a randomly selected surface of 1m x 1 m, above-ground
plant matter was collected, transported to the laboratory and dried in a drying oven at a
13
-------
temperature of 100 C for three days. The amount of dry weight plant biomass/m2 was
determined by weighing the plant matter after drying.
14
-------
D. Analytical Methods
1. Soil/waste Chromatographic Analysis
The principal analysis performed on the soil/waste samples involved determination
of aliphatic (F-l) and aromatic hydrocarbons (F-2). Replicate samples of the waste/soil
mixture were used to form a composite and 2.0 g aliquots of that composite were
subsampled and extracted with 10.0 ml of dichloromethane (DCM). A portion (100}il) of
the extract was placed on a silica gel column for sample cleanup and fractionation. The
column was eluted, first with hexane to remove the trapped aliphatics (F-l), and then with
30% DCM in hexane to elute the polynuclear aromatics (PAH). The two liquid fractions
were analyzed in a gas chromatograph with a flame ionization detector (GC/FID; Hewlett
Packard 5890, equipped with a 50 m x .32 mm DB-5 phenyl methyl silicone capillary
column). Each compound peak was quantitated and identified by GC/MS (Hewlett Packard
5970B, directly interfaced with another HP 5890 GC). The identities of several unknowns
were elucidated in this manner. Data of mesocosm samples containing the commercial
inoculum was presented by GC/MS total ion chromatograms (TIC) using a 25 m x 0.17|im
film 5% phenyl methyl silicone column (0.32 mm ID). Oven temperature started at 50 C for
3 min and increased by 8 degrees C per min to a final temperature of 280 C held for 4 min.
A complete protocol for GC determination of the Fl and F2 constituents is presented in the
Federal Register/Vol. 49. (22).
2. High Performance Liquid Chromatography (HPLC)
Aliquots of the soil/waste mixture (2.0 g wet weight) were weighed, placed into
clean 25 ml scintillation vials. 20.0 ml DCM were added. After mixing by hand-shaking,
the vials were sonicated for 12 min and stored over night at 4 C to let the particulates settle.
With a transfer pipet, 3.0 ml liquid were transferred from the vial into a syringe barrel,
attached to a Millex-SR 0.5JJ. non-sterile PTFE filter. This solution was pushed through the
filter into a 3 ml autosampler vial, capped with a septum cap using a teflon septum. A 10
jil aliquot of that extraction was injected into a Waters HPLC 840 System. Integration of
15
-------
area counts was based on a 3-pt least-squares standard curve, not forced through zero. The
detector used was a fluorescence detector (FL, gain : 2), using an excitation wavelength of
254 nm, and emission wavelength of 375 nm. The column used was a Waters Radial
Compression Separation System (RCSS) column, 5 mm internal diameter (I.D.) x 10 cm;
10 Jim particle size. Separation of PNA's was achieved using a 40 min. water/acetonitrile
(ACN) linear gradient beginning at 35% ACN and ending at 100% ACN, and held for two
min. before reequilibration.
3. Moisture andpH
Analyses of soil moisture and pH were conducted weekly for a total period of 84
days for the task II studies and 184 days for the task HI studies.
The % soil moisture was determined over the whole course of the experiment by weighing
out approximately 20 g of wet soil and drying it in a Blue M constant temperature cabinet
at 120 C for 24 h. The % moisture was determined using the following formula:
Wet weight - dry weight x 100
dry weight
16
-------
IV. Results
A. Pure Waste Analysis
Tables 3 and 4 present data on the analysis of the "pure wastes", classified as
"buried waste" and "lagoon waste" and the river silt (control). About 24 PNA's were
identified (F-2 fraction) (Table 3) and 22 aliphatic hydrocarbons (F-l fraction) (Table 4).
Quantitation was by external standard GC in all cases. Some analyses were semi-
quantitative due to problems in obtaining accurate external standards and the large
dilution factors necessary to prevent GC detector saturation. Detection limit for both
fractions was 100 ppm due to the large dilution factors and the lower response factors of
the higher molecular weight components. The detection limit for the river silt, however,
was 1 ppm.
Table 5 gives the analysis of the total carbon of the soil in % carbon. It was
important, that the indigenous carbon content of the soil be low, in order to guarantee
that the toxicant-carbon be used as prefered source of carbon and energy by the soil
microbial communities.
B. Screening tests
The optimal loading rates for the soil/waste mixtures were determined by
evaluation of both indigenous microbial population performance and acute toxicity
leachate analysis. Microbial ATP analysis showed initial stress on the indigenous
microbial populations through day ten for both waste types (Fig. 4). The microbial
populations had recovered by day 17. Lagoon wastes, applied at 4% and 8%, showed
minimal stress, and microbial ATP levels increased by day 17. Buried wastes showed
comparable results for 2-4% waste loadings at day 17. Microbial diversity indices agreed
with ATP predictions, which suggested maximum loading rates of 8%, for lagoon
wastes, and 2-4% for buried wastes (Fig. 5). EC$Q levels of leachates (Fig 6) predicted
4.0-9.5% waste weight (wet basis) for lagoon waste and 2.5-6.0% waste weight (wet
basis) for the buried waste.Two soil mixtures, soil type I, comprising of one part river
17
-------
TABLE 3: Primary F-2 Constituents in CERCLA waste site
Buried Lagoon River
Compound Wastes Wastes Silt
(control)
1. naphthalene
2. 1-methylnaphthalene
3. 2,3-dimethylnaphthalene
4. 2,3,5-trimethylnaphthalene
5. biphenyl
6. fluorene
7. dibenzothiophine
8. phenanthrene
9. anthracene
10. 1-methylphenanthrene
11. fluoranthrene
12. pyrene
13. benzo(a) anthracene
14. chrysene
15. benzo(b) fluoranthrene
16. benzo(e) pyrene
17. benzo(a) pyrene
18. perylene
19. acenapthene
20. substituted benzene
21. indenopyrene
22. benzo(g,h,i) perlene -^
23. dibenzo(a,h) anthracene
24. benzo(k) pyrene
25. unknown (E.T. = 24.51)
920
1200*
600*
600*
1700*
850
800*
4528
2257
800*
930
830
470
340
ND
ND
<100a
2300
1500*
1100
1200
100 +
ND
ND
1500
1700*
1000*
700*
1600*
1300
1200*
5757.
3227
600*
1686
1059
570
550
ND
60
215
1466
2100*
321
1432
357
457
trace
trace
trace
trace
trace
trace
-------
TABLE 4: Primary F-l Constituents in CERCLA waste site
Compound
C-12
C-13
C-14
C-15
C-16
C-17
PRISTANE
C-18
PHYTANE
C-19
C-20
C-21
C-22
C-23
C-24
C-25
C-26
C-27
C-28
C-29
C-30
C-31
Buried
Wastes
800 ppm
1100 ppm
1100 ppm
1400 ppm
1700 ppm
1400 ppm
4300 ppm
1200 ppm
2700 ppm
750 ppm
370 ppm
240 ppm
Lagoon
Wastes
2300 ppm
3600 ppm
4000 ppm
4300 ppm
5000 ppm
4400 ppm
3100 ppm
4900 ppm
1500 ppm
3300 ppm
2900 ppm
2000 ppm
1400 ppm
840 ppm
600 ppm
890 ppm
300 ppm
320 ppm
*
1200 ppm
560 ppm
330 ppm
Below detection limit of 100 ppm
Lab contaminant
19
-------
TABLE 5: TOC analysis (% carbon) of (he 4% lagoon and 4% buried waste mesocosms over 35 day
(ime frame
TIME
4% LAGOON TL41
4% BURIED (B41
CONTROL SOIL
NJ
O
pure waste
dayO
day?
day 14
day 21
day 28
day 35
day 42
(after
reapplication)
22.847
6.256
5.620
5.625
8.110
9.228
' 4.753
12.197
-
5.295 0.771
4.777
4.152
4.831
5.080
4.816
8.395
-------
Fig. 4: Microbial ATP profile of screening test mesocosms
DAY1
0% SOIL TYPE 1
12% BURIED
"j 8% BURIED
55 4% BURIED
O 2% BURIED
212% LAGOON
^ 8% LAGOON
4% LAGOON
2% LAGOON
0 10 20 30 40 50 60 70 80 90 100110120
RELATIVE LIGHT UNITS ( x 100/g dry wt soil)
DAY 10
0% SOIL TYPE 1
12% BURIED
"j 8% BURIED
m 4% BURIED
DC
« 2% BURIED
c£
dl2% LAGOON
^ 8% LAGOON
4% LAGOON
2% LAGOON
33
0 10 20 30 40 50 60 70 80 90100110120
RELATIVE LIGHT UNITS (x 100 /g dry wt soil)
DAY 17
0%SOILTYPE1
12% BURIED
$ 8% BURIED
2 4% BURIED
^ 2% BURIED
=i 12% LAGOON
5? 8% LAGOON
4% LAGOON
2% LAGOON
0 20 40 60 80 100 120
RELATIVE LIGHT UNITS (x 100/g dry wt soil)
21
-------
Fig. 5: Microbial diversity of screening test mesocosms
01
OT
<
til
oc
o
08
SOIL7YPE1
12% BURIED
8% BURIED
4% BURIED
2% BURIED
100 200 300 400 500
COLONY FORMING UNFTS
600
• BAC
0 PGI
01
Ol
oc
SOILTYPE1
12% LAGOON
8% LAGOON
4%LAGOON
2%LAGCON
100 200 300 400 500 600
COLONY FORMING UNITS
BAC
R3
BAC: Bacteria & actinomycetes (x 10 /g dry wt)
FGI: Fungi & yeasts (x 10 /g dry wt)
22
-------
Fig. 6: Microtox™ analysis of WSF of screening test mesocosm
SOILTYPE1
w 12% LAGOON
O)
<
UJ
§ 9%LAGOON
08
6%LAGOON
3% LAGOON
0 20 40 60 80 100 120 140
SOILTYPE2
uj 12% BURIED
03
<
LU
O 9% BURIED
•a
3? 6% BURIED
3% BURIED
51.3
232.
0 20 40 60 80 100 120 140
EC 50
23
-------
silt and two parts sandy clay (1:2), and soil type II, comprised of two parts river silt and
one part sandy clay (2:1) were tested. Soil type n, when mixed with the waste, was
found to be unsuited for subsequent investigations, because the indigenous microflora
exhibited stress when exposed to that mixture. Soil type I, when mixed with the waste
did not impose stress and was therefore selected for subsequent task n and task m
studies. Loading rates of 4% for lagoon waste and 2.5% for the buried waste were,
therefore, shown as mean optimum loading rates. These rates of waste application were
therefore used in task II mesocosm studies. A 4% O&G loading rate of lagoon waste
required 799.9 g of pure waste sludges to be incorporated into soil mixture I (1.5 kg
river silt mixed with 3 kg sandy clay). Soil waste preparations for 4% O&G buried
waste mesocosms required 274.0 g of pure waste sludges, for 2.5% O&G buried waste
167.4 g and for 2.5% O&G lagoon waste 468.7 g. All these quantities were determined
for 4.5 kg of soil mixture L
C. Mesocosm Tests
2. Chromatographic Analysis
Fig. 7 shows GC/FID chromatograms of the F-2 fractions for lagoon waste
mesocosms with a 4% loading rate and the control soil All compounds show a decrease
over the 35 day incubation period The total gross removal over this time period is
almost 70%. Fig. 8 presents GC/FID chromatograms for the aliphatic fraction (F-l) for
4% lagoon mesocosms over the same time period. The total removal is 90.85%. The
same degradation pattern, but at a faster rate is observed for the 2.5% Lagoon waste
loading (Fig. 9). The total removal for the 2.5% loading F-2 fraction is 96.95% (Table
6).
24
-------
1C/1 __ ai ysis
day time frame
-„-• 4'.. .agt_..
d-0
,, F- . _rat._..i o._- a
Phenanthrene
Anthracene
•.AW..lW*. _,v.
d-7
_• t_
-Av+AJPlAv^iMj
d!4
d-28
to
L/uw^JLMukAJt^A^A^
d-35
Control
Time 5.0 mln
Time 26 mln
-------
Fig. 8: GC/FID analysis for 4% lagoon waste, F-l fraction over a 35
day time frame
C-15
d-0
n.A C-16 c.1? C-19
1-14 v"*'/^ifl /~« if\
C-13 I - C'18 I C-20
jL^^JLAA^ojJwAviXJ^^^
d-7
^A^^—.». K ^. r -vA Jvi^ AA<_)l^*-ir-tA*N-^-. ,M^ ^Jl—, ^A> IM ^^ A>. ^ l|_
d-14
ju^JU/vJJWvi^^
to
-J 1.
' * '
d-28
.^^^JLrAMU^/U^
-1 1 lu
d-35
*
'*''**• * *
Control
Time 5.0 min
Time 34.25 m
-------
Fig. 9: GC/FID analysis for 2.5% lagoo /aste, F-2 fraction over a
d-0
35 day time frame Unk. 15.3 Phenanthrene
II Anthracene
d-14
to
d-21
d-35
• ' ' '
^^jj^^^
^
1 - 1 - 1 - 1 - 1 - 1 - 1 - ' - "
,Ju^_«A*>rV--K*_A^~A>Ai— J LJ^v
>*k4*«r*_/V*
«..K X.
I I I I 1 1 1 1 1 1-
.•.> .Av
Time 7.0 min Time 28.0 min
-------
TABLE 6: F-2 fraction; 2.5% lagoon waste.
Compound 0
Total (Gross)
Time in days
7 14
1900
860
710
21
340
35
NAPHTALENE
METHYLNAPHT.
DIMETHYLNAP.
UNK (Rt-11.1)
UNK (Rt=11.4)
TRIMETHYLNAP.
ACENAPHTALENE
UNK (Rt=12.7)
UNK (Rt=13.2)
BIPHENYL
FLUORENE
UNK (Rt=15.3)
UNK (Rt=15.8)
UNK (Rt=15.9)
UNK (Rt=16.2)
DIBENZOTfflO.
PHENANTHRENE
ANTHRACENE
UNK (Rt=18.4)
UNK (Rt=18.9)
UNK (Rt=19.0)
1-METHYLPHEN.
UNK (Rt=19.9)
UNK (Rt=20.8)
FLUORANTHENE
PYRENE
18.0
19.0
13.0
28.0
25.0
7.0
20.0
82.0
48.0
19.0
30.0
170.0
50.0
21.0
24.0
21.0
138.0
97.0
33.0
27.0
33.0
17.0
51.0
28.0
37.0
1.0
>0.1
>0.1
>0.1
16.0
14.0
0.6
11.0
64.0
39.0
12.0
13.0
120.0
32.0
>0.1
15.0
8.0
68.0
42.0
36.0
16.0
18.0
11.0
33.0
17.0
22.0
11.0
>0.1
>0.1
>0.1
>0.1
1.0
>0.1
3.0
42.0
20.0
5.0
3.8
119.0
36.0
>0.1
12.0
7.0
42.0
38.0
44.0
13.0
18.0
12.5
39.0
22.0
32.0
20.0
>0.1
>0.1
>0.1
>0.1
>0.1
>0.1
1.6
39.0
15.0
1.2
>0.1
75.0
25.0
>0.1
>0.1
>0.1
7.8
22.0
22.0
2.9
5.2
4.4
9.5
9.0
14.0
11.0
>0.
>0.!
>0.!
>Q..
>0.
o.:
u
o.:
>0.1
O.f
13.C
>0.1
>0.1
>0.1
>0.1
1.3
4.2
3.3
0.3
>0.1
0.7
>0. 1
>0 1
4.0
13.0
58
NOTE—The above numbers are accurate to only 2 significant fig.
concentr. in ppm
28
-------
2. MicrobialATP
Microbial ATP contents of the soil/waste mesocosms were monitored over the
course of the experiment. Over the first 35 days, the microbial activities of all
experimental mesocosms in the 4% L-series were higher than those of the control
mesocosms (Fig. 10). Gradual biodegradation was evident during the first 14 days. The
highest microbial activity was evident on day 21 for the lagoon mesocosms, which
correlates with the kinetics of toxicant disappearance. At this point, the indigenous
microflora have adapted to the waste.
Fig. 11 shows the ATP activity for 1L2 - 3B2 as compared to the controls over
the first 35 days of the experiment The microbial activity was initially high in all
experimental units, with a characteristic suppression of growth on day 14. The low
activity in the control mesocosms after day 2 was probably a result of nutrient depletion,
while the subsequent increases in activity at days 28 and 35 resulted from the application
of another nutrient amendment at day 27. Partial reason for the decrease in activity at day
14was moderate desiccation of the mesocosms. The moisture at this day had dropped
to less than 9% for the buried mesocosms. It has been reported in the literature, that
microbial activity becomes marginal once moisture drops to less than 10% water holding
capacity (9). Rewetting of the mesocosms with sterile deionized water increased the
moisture level. Good recovery, indicating the re-adjustment of the microorganisms to the
waste, was evident at day 21.
3. Microbial diversity
Microbial diversity estimates for laboratory mesocosms, as calculated by
determining the mean number of CFU on triplicate plates is presented in Fig. 12. At day
21, the experimental mesocosms exhibited significantly more growth than the control
mesocosms. Counts were high for all mesocosms throughout the experiment indicating
a microbial consortium present at all times. Further monitoring of microbial diversity
through day 84 is presented in Fig. 13. The fact, that such a high density as well as
29
-------
RELATIVE LIGHT UNITS /g dry wt soil
111
RELATIVE LIGHT UNfTS /g dry wt soil
Tl
(5*
11
-•• »N|
.*>.
o
3
CT
5T
TJ
•D
o
«*»
to
(Q
o
o
3
3
-------
RELATIVE UGHT UNITS /g dry wt soil
RELATIVE LIGHT UNITS /g dry wt soil
o
OJ
o
en
o
at
111
-«. -4 10
o
o
cr
si!
>
TJ
-i
o
—Jj
CD"
o^
10
(O
o
o
3
O
(0
o
o
o
0)
3
(0
"E
c
to
O
Q.
0)
ro
-------
Fig. 12: Microbial diversity of mesocosms plus C1, day 7-35
8
C3
|
X
§
•a
§
CO
CS1 CS2 CS3 1L4 2L4 3L4 C1
MESOCOSMS
I
O
•0
d
CS1 CS2 CSS 1L4 2L4 3L4 C1
MESOCOSMS
DAY?
DAY 14
DAY 21
DAY 28
DAY 35
32
-------
Fig. 13: Microbia! diversity of mesocosms, day 28-84
O
W
GQ
1
O
O)
50
~ 40 H
£
2 30H
CS1 CS2 CS3 1L4 2L4 3L4 1B4 2B4 3B4
MESOCOSM
50-i
40-
30-
D84
D77
CS1 CS2 CSS 1L4 2L4 3L4 1B4 2B4 3B4
MESOCOSM
33
-------
diversity of all four general groups of microorganisms was maintained, indicated that
the requirements of a genetic pool to emphasize biodegradative kinetics were evident
Microbial diversity of fungi and yeasts were generally comparable to the bacterial
counts. Fungal counts were high throughout the whole course of the experiment, while
the yeasts were always present in low numbers. This relationship is characteristic of
many agricultural soils (51). The SPC-plate counts for fungi and yeasts indicated a well
adapted fungal population with highest overall counts in the 4% and 2% lagoon
mesocosms. These mesocosms seemed to provide an extraordinarily good habitat for the
fungi. Generally, SPC data reflects only variations in colony forming units and does
not provide interpretations on either total biomass or actively viable biomass. It was
intended to only reflect the transient microbial morphology of control and amended
soils during the experimental time frame indicated so as to insure that a genetic pool
capable of toxicant biotransformation was present at all times. At no time during the
experiment was there evidence of acute or chronic toxicity to these adapted microbial
populations.
4. Microtox™
Microtox results were provided by EPA laboratories in Ada, OK. Microtox™
toxicty results for the WSF fractions of the mesocosms over a 35 day time frame are
presented in Fig. 14. Data, shown as LL5 value, is defined as the relative mean % loss
of bioluminescence in the WSF samples over a five minute time period. The 20% value
marks the threshold between the non toxic state (LL5 < 20%) and the toxic state (T.T.5 >
20%). If LL5 values were found to be less than -5%, this was reported as stimulation of
bioluminescence. Stimulation was observed for the control mesocosms (CS) from day
zero to day 35. For all the other experimental mesocosms of the 4% - and 2.5% loading
rates, TJ-S values were constantly high indicating a highly toxic response by the test
bacteria. Technician error may have contributed to inaccuracies in the analysis of this
data set Additional sources of error may have been introduced by various interferences
34
-------
LL5 (Relative
Mean % Loss of
Blolumlnescence
In WSF Samples) 20..
Fig. 14: Microtox Toxicity
Results of Mesocosms
ics
1L4
I L2
EJB4
DB2
Day
35
-------
with the Microtox™ test system. The test organism, being a marine microbe, may have
been repressed by intermediates formed in the soil or partially solubilized metals present
in the river silt Plating studies and microbial activity estimations of the adapted,
indigenous microflora did not exhibit repression over this time period. For these
reasons, Microtox™ is still being evaluated for assay of oil-contaminated terrestrial
ecosystems (Matthews, personnel communication). Despite the Micotox™ results, EPA
approval was obtained for the field verification studies; decisions were based on GC/MS
and microbial activity data.
5. Commercial inoculum test
Fig. 15 shows a series of Total Ion Chromatograms (TIC) of the mesocosm
inoculated with the commercial inoculum (Cl). This mesocosm contained 4% lagoon
waste plus commercial inoculum.The upper TIC chromatogram presents analysis for
day zero while the middle TIC chromatogram presents analysis for day 35. Separation
into aliphatic and aromatic hydrocarbons was not possible when preparing these samples
for GC analysis. High biomass interferences were noted.When comparing the upper
two chromatograms to the PAH standard (lowest TIC), it was evident, that all of the
compounds analyzed exhibited a decrease in concentration over time. The appearence of
unresolved compounds as expressed in the high baseline for day 35 was probably due to
complex organic molecules produced by the microorganisms. This was consistent with
our difficulties in F-l/F-2 fractionation as mentioned and further documented by HPLC
(not represented here).
The degradation rates for acenapthene, anthracene and phenanthrene by the
indigenous (adapted) and commercial inoculum are shown in Fig. 16 and Fig. 17. The
commercial inoculum showed an enhanced degradation rate for these compounds over
the first 14 days of the experiment The rate of degradation was almost linear for this
time period observed. The degradation pattern for the 2.5% lagoon waste began at a
similiar kinetic rate and slowed slightly for anthracene and phenanthrene. Acenapthene
36
-------
Fig. 15: Total ion chromatograms of mesocosms with commercial
inoculum
TREATMENT OftY-0
nc a
B. BC4-]
B.BC4
7. BC4
i.BC4
3.BC4
4.BC4
3.BC4
f V3iCL?.Q
1BQQQ
a
TREATMENT OAY-35
TIC of V3.CL43.D
SBBB
4BBB
aaaa
aaaa
1QOB
PftH STANDARD 25 NG
TIf .
l.BEd
1.4C4-
t.SC4;
laaaa;
BQQO;
SDpQ;
SBBQ:
' va'ST«'
*^. •
° VP
Q
1
9Q
1
.
SB dQ
37
-------
Fig. 16: Degradation rates for commercial inoculum as compared
4% adapted inoculum
100
Commercial Inoculum
•a-
-»- ANTHRACENE
•» PHBMANTHFBvE
10 20
Time (days)
30 40
4% LAGOON
100
Adapted Inoculum
10 20
TIME (days)
•o ACBVJAPTHALENE
-^ PHBJANTHRBE
-B- ANTHRACENE
30 40
4% LAGOON
38
-------
Fig. 17: Degradation rates for commercial inoculum as compared to
2.5% adapted inoculum
100-
Commercial Inoculum
10 20
Time (days)
-a- ACBMAFTHEM
-»- ANTHRACENE
«• PHENANTURB
30 40
4% LAGOON
a
Z
z
LU
CC
LU
QC
(ft
100-
80-J
60 i
40-
20-
ACENAPHTENE
ANTHRACENE
PHENANTHFe
0 7 14 21 35
TIME (DAYS) 2.5% LAGOON
39
-------
was degraded at a linear rate (Fig. 17). This biodegradation response, at 2.5% O&G,
would be expected since the waste concentration was lower and consequently less toxic
than the 4% loadings. The adapted mesocosms at the 4% load show an initial lag task.
No degradation was observed over the first 14 days of the experiment. Following day
14, however, there was a significant increase in toxicant degradation rates. Both rates,
at concentrations at or approaching 20% of the original toxicant toxicant addition for both
adapted and commercial inoculum, leveled out over time. Final residual concentrations
for both inocula were similiar. At the conclusions of these investigations, no noticeable
variation in biotransformation/ degradation by the commercially available mutated
bacterial cultures over the adapted cultures was evident
6. Sequential Re-loading of 4% and 25% Mesocosms
After substantial waste reduction had been evident in the first 35 days of the experiment,
the 4% and 2.5% mesocosm series were reloaded with identical waste concentrations.
Soon after waste incorporation, it became evident, that the 4% lagoon mesocosms were
overloaded as indicated by decrease in microbial activity (Fig. 13). The soil/waste
mixture was too heavy and dough-like. More soil was therefore incorporated into the
mixture to compensate and render it more workable. A 4% reloading rate was deemed
too high for subsequent applications. It was observed from data generated in the 2.5%
reloading experiments, that 2.5% would be an acceptable rate for waste re-applications in
the given time frame. Subsequent monitoring of microbial ATP and microbial diversity
reconfirmed no induced stress of the microbial communities under these waste
concentrations.
7.HPLC
High Performance Liquid Chromatography (HPLC) analyses were performed
weekly to monitor general trends in toxicant concentrations. Fluorescence detection
using an excitation wavelength of 254 nm, and emission wavelength of 375 nm, was
used in all cases. Peak identification was by comparison with PAH standards. Several
40
-------
major problems rendered interpretations of data sets difficult. The major problem
consisted of poor reproducability of replicate samples due to mechanical error on
autosampling coupled with sample variability. Thus, for example, significant sampling
differences were noted for the 2.5% O&G buried waste, while other waste treatments
showed similarities between the individual replicate mesocosms. Achieving the
necessary homogenicity in test samples was not possible due to unfavorable mixing
properties of the oily sludges with the soils. Also observed was an increase in baseline
of the respective chromatogram caused by accumulation of unresolved polar organic
compounds on the column. This phenomena was also observed by GC/MS and will be
discussed in section "discussion". Another problem associated with the use of
fluorescence detection was that anthracene, one of the key compounds, saturated the
detector at concentrations more suitable for the detection of other PNA's. This was due
to its high fluorescence response compared to other PNA's. Generally, HPLC results
for 4% and 2.5% loading rates illustrated similiar trends in waste reduction up to day
35. After reloading, however, no continued reduction was evident indicating that the 4%
sequential load was excessive. For these reasons, this data was not presented in the
context of this report However, all HPLC samples were retained and will be re-analyzed
at such time when equipment limitations have been corrected. Appendix C shows HPLC
chromatograms for the buried waste (1B4,2B4,3B4). These are presented to indicate
trends only.
8. Soil Moisture andpH
These parameters were monitored consequently over the whole course of the
experiment. No significant changes in pH were observed, while moisture fluctuations
were controlled by periodic addition of sterile water. Again, it was noted, that %
moisture below correlated with ATP responses. Higher microbial activity was always
observed when moisture contents were at maxima. The pH, however, did not seem to
have an effect The results are presented in Table 7 andTable. 8.
41
-------
TABLE 7: % Moisture of Laboratory Mesocosms
Time in days
Mesocosm 25 7 10 14 21 28 35
N>
CS1
CS2
CS3
1L4
2L4
3L4
1B4
2B4
3B4
1L2
2L2
3L2
1B2
2B2
3B2
B20
B21
L20
L21
Cl
C2
3.00
5.50
3.85
22.78
19.56
19.80
7.50
5.28
6.52
9.21
8.19
9.01
6.10
8.29
6.34
1.49
8.05
4.15
2.18
1.41
3.24
6.68
10.62
18.54
26.45
22.14
24.57
10.00
8.86
8.30
17.22
15.68
16.52
11.36
11.93
14.32
8.05
11.73
8.39
7.01
8.52
8.54
7.01
12.10
9.66
29.47
23.27
24.98
8.57
7.91
8.39
9.86
14.83
17.86
10.41
11.71
13.42
13.67
15.49
27.08
25.01
24.11
12.56
7.78
11.24
9.85
29.45
24.42
26.25
10.86
9.59
8.92
20.95
14.81
18.75
13.05
12.08
14.04
13.75
17.28
28.62
24.36
27.33
13.12
3.78
7.09
6.18
20.60
16.54
22.16
6.79
6.69
7.07
11.52
10.63
15.09
8.99
8.67
13.05
8.10
11.53
21.33
19.76
13.88
10.31
6.63
12.28
10.04
24.86
20.37
23.42
8.22
8.37
8.77
16.10
13.77
17.53
11.31
11.14
17.41
23.21
14.11
27.70
24.96
15.91
13.65
8.84
12.58
9.63
27.69
22.61
22.83
8.26
9.00
9.04
15.63
13.83
18.40
11.48
13.78
13.90
12.91
14.83
23.88
30.11
23.54
12.59
8.76
10.71
9.24
23.81
15.83
18.54
7.26
7.61
7.76
13.88
11.75
13.72
10.18
13.72
11.55
9.81
13.73
28.23
38.34
17.18
12.02
-------
TABLE 7 (cont'd):
Mesocosm
% Moisture of Laboratory Mesocosms
Time in days
42 49
77
84
CS1
CS2
CS3
1L4
2L4
3L4
1B4
2B4
3B4
1L2
2L2
3L2
1B2
2B2
3B2
B20
B21
L20
L21
Cl
C2
9.45
13.59
11.83
37.75
37.75
35.30
12.48
7.71
6.37
14.12
15.96
18.82
11.49
12.77
12.42
10.86
12.23
24.10
19.91
21.41
12.18
10.47
37.66*
37.34
38.21
13.13
12.23
10.80
-
-
_
.
-
5.99
6.69
8.27
34.41
33.72
45.21
10.99
10.92
9.01
-
-
-
-
-
5.81
6.67
9.25
29.88
32.44
31.33
7.45
10.01
8.57
-
-
.
_
-
* After waste re-application for 1L4 - 3B4.
-------
ol
TABLE 8: pH Profiles of Laboratory Mesocosms
Time in days
Mesocosm 257 10 14 21 28 35
CS1
CS2
CS3
1L4
2L4
3L5
1B4
2B4
3B4
1L2
2L2
3L2
1B2
2B2
3B2
B20
B21
L20
L21
Cl
C2
8.25
7.82 '
8.17
7.28
7.21
7.23
7.90
7.91
7.96
7.34
7.38
7.45
7.55
7.58
7.54
3.42
8.14
8.55
8.44
8.4
8.5
6.05
6.87
6.87
6.93
6.95
7.11
6.05
6.64
7.52
5.86
5.79
6.99
6.94
6.97
5.88
5.65
6.89
7.17
7.16
6.99
6.87
7.23
7.25
8.34
6.78
6.28
5.94
6.11
6.85
6.67
6.52
6.50
6.50
6.40
6.43
6.40
6.95
6.90
6.23
6.63
6.13
7.16*
8.55
7.56
8.12
6.39
6.51
6.53
6.82
6.81
6.75
6.75
6.81
6.56
6.57
6.56
6.54
6.53
6.54
6.42
6.51
6.48
6.72
8.40
7.89
7.86
6.61
6.58
6.55
6.97
7.07
7.01
6.76
6.78
6.81
6.77
6.80
6.67
6.58
6.59
6.69
6.58
6.58
7.08
8.05
7.52
7.98
6.70
6.63
6.93
7.06
7.02
7.01
6.79
6.80
6.91
6.87
6.80
6.88
6.91
6.64
6.72
6.98
6.90
7.30
8.38
7.59
7.74
7.41
7.06
7.19
7.21
7.07
6.98
7.30
7.40
7.36
7.26
7.13
6.97
6.74
7.13
7.15
7.03
7.26
7.42
8.22
7.77
8.25
7.73
6.83
6.93
7.61
7.91
7.66
8.34
7.37
8.00
7.36
7.33
6.94
7.27
7.02
6.91
6.89
7.13
7.71
* Waste application at day 6.
* pH measurements: Ig soil in 99ml distilled deionized H20
-------
ui
TABLE 8 (cont'd): pH Profiles of Laboratory Mesocosms
Time in days
Mesocosm 42 49 77 84
CS1
CS2
CS3
1L4
2L4
3L4
1B4
2B4
3B4
1L2
2L3
3L2
1B2
2B2
3B2
B20
B21
L20
L21
Cl
C2
7.98
7.63
7.94
6.95
6.90
6.79
6.77
6.96
6.74
7.42
7.44
7.53
7.45
7.35
6.89
6.48
6.62
6.58
6.85
6.72
6.97
8.31
-
-
* 7.02
7.26
7.20
7.06
7.08
6.91
_
-
-
_
-
-
_
-
_
-
.
™
8.12
7.38
7.85
6.97
6.88
6.92
6.28
6.10
6.08
»
-
-
_
-
-
_
-
_
-
_-
~
7.87
7.52
7.75
7.24
7.06
6.97
6.50
6.31
6.26
_
_
-
^
_
-
_
-
_
-
_
••
* After waste re-application for 1L4 - 3B4
-------
D. Field Verification Studies
1. Chromatographic Analysis
Field studies, coordinated under IT personnel, were conducted at the Old Inger
site. Data from pilot verification studies is shown in Fig. 18. These GC/FID chromatograms
represent analysis of the first waste application for both group I and group n plots over a
25 day time frame. The first chromatograms of each series were from the control plot
before waste application. Complete differentiation into the two fractions, F-l and F-2, was
not possible, because the waste was different in hydrocarbon composition than the waste
used in the previous task n mesocosm studies. Additionally, the waste contained a lesser
amount of PAHs than the one tested in the laboratory. A composite of the F-l and F-2
fractions therefore was used for analyses. However, this did not pose a problem from
analytical detection capabilities involved, but supported GC/MS data of original wastes
being predominantly F-l. The general trend of hydrocarbon reduction as observed in task
n studies was evident It was important to note, that the residual components, found at day
25, were determined by GC/MS to be primarily F-l straight chain hydrocarbons,
respectively C-17 and C-19 aliphatics.
Subsequent re-analysis of F-2 fractions at the end of the experiment were
conducted for group I and n where group I analysis is presented in Fig. 19. On day 65,
only a small residue was left as a consequence of the first waste application. Reloading
group I with 2.5% buried waste was completed on day 102. This additional waste
application at day 102 was responsible for the increase in hydrocarbon content by day 127
as compared to day 65. By day 156, a further increase in F-2 content along with substantial
biotransformation as expressed by increase in baseline was obvious. This unexpected
increase could probably be attributed to sample variability and poor mixing of the soil/
waste components in the field plots.
Subsequent group n re-analyses of F-2 fractions are presented in Fig. 20. On day
46, first reloading of these plots with 2% lagoon sludge was applied. Samples analyzed
46
-------
IS
group I
before waste application
Fig. 18: GC/FID analysis for group I and group II over a 25 day
time frame
d-l
d-25
^> ^JU>
Time 7.0 min IS=Intemal Standard
group
before waste application
Time 19.0 min
d-25
-------
FIG. 19: GC/FID analysis for group I, F-2 fractions, day 65 -156
day 65
oo
Time: 10.0 min
day 127
Time: 10.0 min,
_J • >
PNA STD
' ' [ ' f.
Time: 10.0 min
Time: 41.0 min
Time: 41.0 min
.11
• ' •
Time: 41.0 min
-------
FIG. 20: GC/FID analysis for group II, F-2 fractions, day 46-156
day 46
il i Al , A A , J I
-vUwuAKK_K^_
Time: 10.0 min
day 65
Time: 41.0 min
Time: 10.0 min
day 79
Time: 41.0 min
Time: 10.0 min
Time: 10.0 min
Time: 41.0 min
Time: 41.0 min
-------
on day 65 showed a consequent reduction of waste. The noticeable increase by day 79
without additional waste application is probably due to sample variability, or collection
from an alternate part of the fieldplot. Sample variability was one of the major problems of
the experiment and will be discussed in Section V, Discussion. The chromatogram of day
102 reflects the third waste application for these plots. This was the first time the plots
received a load of buried waste (2.5% O&G). The mixing of buried with lagoon waste in
the same plot rendered tracing the fate of one or more individual key toxicant significantly
more complicated. GC chromatogram interpretations were impeded, because general
disappearance patterns of the toxicants were no longer apparent Hydrocarbon
accumulation was observed from day 127 over day 156 to the end of the investigations.
This indicated overloading of these plots as had also been confirmed by Microtox™ (and
plant biomass determinations, see section IV, D 6). The fourth waste applicatiori with 2.5%
buried waste on day 156, was therefore excessive.
To conclude, hydrocarbon biotransformation/degradation fates were re-evaluated
after a time period of human inactivcness. Samples were taken four months after the last
activity at the field had been carried out, because there was a concern about overloading of
the group n experimental plots. At this time, plants were established in the field plots.
Samples were analyzed in two increments to a depth of ten to twelve inches to study the
residue levels and downward migration of organics. In addition, four surface samples (0"-
6") were taken from alternate locations of PL5 to evaluate uniformity in samples, and better
explain previous variation in analytical results due to sample and hydrocarbon content
variability. No fractionation into F-l and F-2 fractions was done for these last samples,
because the wastes were already too heavily weathered and metabolized
Plot four exhibited limited toxicant downward migration to a depth of 12 inches
as was confirmed by TOC data (see Fig. 31). No migration was evident for the other
plots up to the tested depth of ten to twelve inches. Fig. 21 depicts a depth profile for
PL5. The large area of unresolved compounds indicates, that substantial
50
-------
FIG. i*. GC/FJD analysis for PL5, 0"-20"
0"-6"
sv
Time: 3.0 mi
mm
Time: 38.0 min
Ul
IS
j I LsX
ul^
Time: 3.0 min
phthalate
Time: 38.0 min
16"-20"
IS
phthalate
Time: 3.0 min
Time: 38.0 min
-------
biotransformation took place in the upper six inches of the soil with only a few
identifiable compounds left. It was of great interest to note therefore, that even with
application of large quanities of toxicants, no significant migration took place into the
deeper soil layers.
Fig. 22 depicts four chromatograms of PL5 from different locations as shown
below.
PLS
It can be seen (Fig. 22), that there was no homogenicity in the samples indicating
poor mixing of the waste with the soils in the field. The tendency to clump and oily
contents of the soil rendered mixing by field engineers difficult Better methods for
future soil/waste incorporations need therefore to be applied to promote uniformity in
samples. Such better methods could also favor microbial biotransformation while
providing a better microbial habitat
2. Microbial ATP
Microbial ATP estimations for the field plots over the total time period of 184
days are shown in Fig. 23 - 25. Day zero is actually the second day after the first waste
application. The microbial activity was depressed through day ten, but recovered well,
by day 23. This recovery was favored by the application of fertilizer, (application rate 5
Ib /plot 10-5-5) and tillage for better oxygenation at day 14 for all five plots. All
fieldplots at day 23 showed higher activity than the control plot (Pll). The group I
experimental plots (P12.P13) (Loaded with 4% buried waste) show less microbial activity
than the group n (P14,P15) Goaded with 4% lagoon waste) experimental plots. There
was also activity suppression observed at day 30. This probably resulted from
reloading of the group n plots with another 2% loading rate. In contrast, group I and the
52
-------
FIG. 22: GC/FID analysis for alternate locations of PL5, 0"-6"
Time: 3.0 min
D
Time: 46.0 min
Time: 46.0 min
Time: 46.0 min
Time: 3.0 min
Time: 46.0 min
-------
RELATIVE LIGHT UNITS /g dry wt soil
111
RELATIVE LIGHT UNITS /g dry wt soil
•n
o
to
ooo
-* o> o
o
to
O)
o
~t
o
g;
o^
5"
o
O
»-••
W
««
a
0)
•<
o
I
CO
-------
Fig. 24: Microbial ATP profiles of field plots, day 51-113
o
(A
CD
UJ
Ul
DC
PL2 PL3 PL4
FIELDPLOT
PL5
DAY 51
DAY 58
DAY 65
b
D
I-
o
10
PL2 PL3 PL4
RELDPLOT
DAY 102
DAY 106
DAY 113
55
-------
RELATIVE LIGHT UNITS /g dry wt. soil
RELATIVE LIGHT UNITS /g dry wt soil
•n
(5*
3
m
§
11
II
^i en
o o>
Ol
O)
_A
09
-------
control plot at this day showed extreme suppression. The cause for this phenomena is
believed to have been an extraordinarily heavy rainfall, associated with a hurricane, that
flooded the fieldplots and rendered them temporarily anoxic. The microbial ATP levels
had recovered well by day 37 for all fieldplots.This good recovery was probably
enhanced by renewed tillage of all plots at day 33. Further activity fluctuations, until day
102 are also believed to have been caused by different aeration conditions induced by
tillage, temperature fluctuations and periodic frost
A third waste application followed on day 102, when both groups were re-
loaded with 2.5% of buried waste. Sampling took place after the waste application, and
counts at this point were lower than those of the control plot No significant changes
were seen over the following four days, a period believed to be a task of adaptation to
the new carbon sources. At day 113 however, significant increases in activity were
observed. A final waste application of 2.5% buried waste was applied to group n at day
156 of the experiment Even though sampling took place after waste application, the
microbial activity for group n, P14 and P15 were significantly higher than that of the
other experimental plots. This unexpected phenomena was probably due to the short
time period between application, sampling and tillage of these two plots on that day,
while no activities were carried out for the other field plots. At the end of the experiment
(day 177-184), group n plots contained significantly higher activity than the control and
group I plots. The latter probably were nutrient depleted or deficient of a bioavailable
carbon source, while the former still were growing on the toxicant carbon source.
3. Microbial diversity
Microbial diversity is presented in Fig. 26-28. Plating studies for bacteria and
actinomycetes reflect the ATP biocounts for day 23. The biomass throughout the
experiment lay in the range of 107organisms/g wet weight soil which is comparable to a
rich agricultural soil No further variations in biomass were observed between day 30
and day 37. At day 51, there was a reduction in microbial activity, but a high biomass
57
-------
Fig. 26: Microbial diversity of field plots, day 23-58
12
o
(A
I
O)
D23
D30
D37
PL1 PL2 PL3 PL4
RELD PLOT
PL5
o
(0
O>
5
T"
X
*
•B
d
CO
12-1
10-
D51
D5S
PL1 PL2 PL3 PL4 PIS
RELD PLOT
58
-------
Fig. 27: Microbial diversity of field plots, day 102-141
12
a>
o
|
x
•
<
•8
CO
10
8
6
4
PL2 PL3 PL4
FIELD PLOT
PL5
D1Q6
D 113
D102
8
i
PL1 PL2 PL3 PL4
RELD PLOT
59
-------
Fig. 28: Microbial diversity of field plots, day 170-184
12
o
at
•J
"55
o>
5
•0
tj
00
10 J
I D170
I D177
I.D184
PL1 PL2 PL3 PL4 PL5
FIELD PLOT
60
-------
was indicated by SPC. It is believed, that the biocounts using SPC could be higher than
expected due to the suggested presence of some spore-forming species. Those, once
inoculated on the Petri dishes having a more readily available carbon source, germinate
and grow out in high numbers, but are relatively inactive in the ecosystem. After the
third waste application at day 156, all experimental fieldplots invariably contained more
biomass than the control plotThis was probably due to the better nutrient supply
provided in the wastes contained in these plots.
4. Microtox™ and TOC analysis
The Microtox™ toxicity results for the WSF fractions of the 0-6 inch soil core
samples are given in Fig. 29. Day 00 indicates analysis of plots before any treatments.
Group I and Group n received waste loadings on day zero of 4% loading rates of buried
waste and lagoon waste, respectively. Samples were collected after waste incorporation.
Group n WSF showed highest toxic responses by the test organisms, indicating this
waste to be the most toxic. The T.T.5 values for Group I were lower than for Group II.
This level of higher relative toxicity for the Group n samples was expected, since more
toxic waste constituents had been found in lagoon waste than in buried waste. Task n
mesocosm studies also confirmed this phenomena. After a period of 45 days, both
groups showed conditions similar to the initial ones (Day 00), which led to the
conclusion, that substantial waste reduction took place during this time period.
Continuing decreases in LL5 values for group I was observed until day 86. At day 102,
the significant increase is in response to a second reloading of buried waste incorporated
that day before sampling. This LT.5 observation would predict another decrease in WSF
toxicity until day 184. The deviation from the expected pattern was believed to be due to
the presence of toxic intermediates and/or partially solubilizcd metals during this time
period. A toxic effect has been observed for the group I samples at day 184 to a depth of
18 inches. This indicated downward migration of soluble constituents, and possible
inhibition of soil degradation processes. Since the control samples fell in the transition
61
-------
Fig. 29: Microtox Toxiclty Results of Reid
Samples (0"-6")
70 j
60 ••
50 ..
LL5 (Relative Mean 40
% Loss of
Bioluminescence In
WSF Samples) 30 +
4% B before sampling
A
4% 8 before sampling
I
2.5% B before sampling
I
B « buried waste
L» lagoon waste
62
-------
range over the same time period, other intermediates or toxic substances may have been
present, which contributed to this toxic response by the bacteria. Microtox™ toxicity
results for group n followed the same pattern as group one, except there were a total of
four alternate waste applications for group n while there were only two waste
applications for group I.
Reduction in LL5 light outputs was observed over the first 45 days of the
experiment, while the subsequent increase in WSF toxicity indicated potential
overloading of the plots. On day 184, downward migration to a depth of 18 inches was
evident, while no toxic effects were observed earlier at this depth. This limited
downward movement is still within the depth of acceptable treatment (Matthews,
personnel communication).
TOG data was provided by West Paine Laboratories, Baton Rouge, LA. TOC
analysis of day 149 and day 184 are depicted in Fig. 30 and 31. Day 184 confirmed
Microtox™ data in that no significant migration of materials was noted with increasing
depth of 18 or 24 inches. With the exception of PL4, where limited downward migration
was evident in a depth of 12 inches, all plots followed the pattern of the control plot
indicating very little vertical migration. This limited migration of PL4 was also
confirmed by GC/MS data. Both analyses indicated no carbon accumulation with
increasing depth. These results demonstrated the usefulness of the TOC assay in
monitoring vertical migration of organic carbons in the soil environment However, this
assay should not be used alone, to estimate soil migration patterns.
5. Soil moisture andpH
Tables 9 andlO depict the soil moisture conditions and pH measurements for all the
field plots over the total 184 days of the experiment for the 0-6 inch-depth intervals. pH
values were mostly neutral or slightly alkaline, a range which normally favors microbial
growth of bacteria. The pH values did not drop nor rise significantly thus imposing little
effect on the soil processes.
63
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Fig. 30: TOO analysis for Day
149
TOO (mg/Vg C)
25000 r
20000 • •
15000- •
10000"
5000- •
fl.1
P12
PL3
-------
Fig. 31: TOC analysis for Day 184
TOC (mg/kg C)
25000 T
20000 •
15000 •
10000
5000 A
12 18
Depth in inches
24
65
-------
TABLE 9: % Moisture of Fieldplots (0-6" depth)
Time in days
Mesocosm 03 10 23 30 51 58 65 102
PL1
PL2
PL3
PL4
PL5
(cont.)
PL1
PL2
PL3
PL4
PL5
17.93
18.61
18.96
22.42
21.55
106
28.53
22.99
23.99
21.45
23.35
26.51
28.76
31.40
31.03
24.42
113
22.56
22.80
22.80
20.33
22.17
23.54
25.42
23.06
21.86
23.38
127
20.74
19.13
17.33
16.66
18.81
26.26
25.33
25.79
28.31
24.77
134
15.55
17.01
17.91
15.60
18.29
20.88
23.50
21.41
22.95
23.82
141
14.44
19.23
16.44
16.45
14.78
18.39
20.45
19.73
24.20
19.48
156
23.42
22.54
22.41
22.40
22.48
19.29
17.84
12.99
20.08
22.44
170
17.36
19.48
18.63
19.81
17.77
26.37
26.99
24.53
23.37
23.92
177
17.23
19.02
15.83
17.43
19.78
18.4
19.8
24.4
24.1
23.3
184
17.1
15.2
18.8
15.5
18.9
-------
TABLE 10: pH Profiles of Field Plots (0-6" depth) Time in days
Fieldplot 0 3 10 23 30 44 51 58
PL1
PL2
PL3
PIA
PL5
(cont.)
Fieldplot
PL1
PL2
PL3
PL4
PL5
65
7.4
7.3
7.3
6.9
6.8
8.08
8.23
8.45
8.16
8.43
102
7.68
7.71
7.25
7.59
7.51
6.17
6.16
6.52
6.44
6.22
106
7.26
7.33
7.34
7.24
7.27
7.00
7.20
7.60
7.50
7.30
113
7.60
7.42
7.65
7.50
7.70
127
7.50
7.50
7.70
7.60
7.50
7.00
7.50
7.80
7.40
7.35
134
7.7
7.7
7.7
7.7
7.5
7.50
7.65
8.02
8.05
7.91
141
7.3
7.5
7.5
7.6
7.5
156
7.9
7.8
7.6
7.8
8.6
.
-
7.61
7.72
170
8.2
8.2
8.4
8.1
8.3
8.21
7.85
7.75
6.71
7.11
177
6.8
6.8
6.8
6.7
6.8
8.25
7.00
7.00
6.90
6.90
184
7.8
8.0
8.1
8.2
7.9
* pH measurements: Ig soil in 99ml distilled deionized H20
verified by measuring with various pH instruments.
-------
The moisture ranged from almost 13%, as the lowest recorded value, up to 31 %.
Moisture contents correlated with microbial activity data. Moisture was not a limiting
factor in the field studies since it never became marginal, however only in combination
with good aeration can it favor microbial growth.
6. Plant Biomass Determination
Since one of the ultimate goals of in situ biological treatment consists of the
establishment of a vegetative cover over the treated areas, it was of special interest to
measure the amount of plant biomass after some time. Four months after the last
experimental treatments at the field plots had been carried out (August 13 -17,1986), the
above ground plant biomass was determined. Varieties of plants indigenous to the area
were growing in the field plots. There was a very gradual, but defined increase in plant
biomass from group n, to group I, to the control field plot The control plot, which had
never been loaded with any toxicants, had substantially more plant biomass than the other
groups. Some individual plants in this plot reached a height of five feet, seven inches.
Plant height in the other plots was generally lower. However, group I plots (PL2, PL3)
also contained individual plants up to five feet tall.. Only small grasses, growing
sporadically were found in the group two plots (PL4, PL5). These two plots also showed
evidence of water logging toward the western edge of the plots, while there was no direct
water logging seen in the other plots. Fig. 32 shows the g plant dry weight /m2.
These plant biomass results correlate with the toxicity predictions of the
Microtox™ measurements. Both indicated an overloading of these plots with the oily
sludges, resulting in toxic conditions for the micro- as well as the macrofiora. A factor
contributing to these toxic conditions especially for the group n plots was probably the soil
composition in the plots, which seems to have been too high in clay and silt content, thus
preventing good aeration for maximum microbial activity and favoring water logging.
However, soil composition was acceptable in preventing downward migration of organics.
68
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Fig. 32: Plant biomass
determination of field plots
600T
500.
400--
g plant dry wV m2
field plot surface
200-
100-
PL1
PL3
Fleldplot
PL4
PL5
69
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In addition, IT representatives indicated hydraulic overloading of the group II
plots, caused by excessive amounts of water that was not separated from the sludge before
applications. Over saturation with water therefore prevented maximum microbial activity
and rendered degradation by indigenous microbial populations incomplete. Over saturation
with water may have also indirectly caused oxygen-deprivation of plant roots thus
rninimizing plant growth in these plots.
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V. Discussion
Bacteria play the major role in hydrocarbon degradation in soil environments. It is
reported in the literature, that most of the responsible bacterial strains are sensitive to
acidity and only little growth is reported at pH values of below six or even five (1). The
soil pH thus plays an important role in hydrocarbon degradation. Most bacteria have limited
tolerance for acidic conditions in the soil, fungi are more resistent (9). Consequently, the
soil pH often determines the types of microorganisms that assist in hydrocarbon
degradation. There is evidence that the overall rate of hydrocarbon biodegradation is
higher under slightly alkaline conditions than under acidic conditions (9). The soils tested
in our field studies were neutral or slightly alkaline. Soils in Louisiana are generally more
basic in nature and well buffered, therefore pH did not impose a problem on the microbial
consortium tested in our investigations. The pH range of our tested soils should, indeed,
favor the growth of the mixed bacterial-fungal communities. No pH lower than six was
measured in our experiment, as can be seen in Table 10.
Abiotic factors, especially temperature and moisture changes contributed to the
relatively great changes in the biological parameters measured. Controlled conditions as
maintained in the laboratory are not achievable in field studies. Since the initial steps of
hydrocarbon biodegradation are oxygen dependant, and the rate of degradation is highest
when aeration is maximized, monitoring and maintenance of oxygenated conditions in the
field are important (9). Aeration of field plot soils was achieved by tillage of the plots and
raking of the surfaces to remove the formed crusts. Large amounts of readily usable
organic substrates, including hydrocarbons, tend to deplete the oxygen reserves of the soil,
especially if small pore spaces or a high degree of water saturation slows oxygen diffusion
(9). Too much moisture in the soil interferes with the availability of oxygen and renders
aerobic microbial metabolic activity marginaL Therefore, it is suggested, that greater tillage
or better mixing methods be applied by the field engineers.
71
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The oxidation of aliphatic hydrocarbons has been reported to occur in the
temperature range from 0 C to about 55 C (1). Aromatic hydrocarbons are also oxidized at
a wide range of soil temperatures, starting from freezing point and optimizing between 30
C and 40 C (8). In experiments conducted by Dibble and Bartha with a New Jersy soil,
hydrocarbon biodegradation was highest at temperatures above 20 C with no further
increase in rate at 37 C. The generally warm local temperatures of the southern Louisiana
region should therefore favor the conditions for landfarming and ISBT for hazardous
waste sites in the region.
To eliminate the interference of other readily available carbon sources in the soil,
that could delay toxicant-carbon uptake, the carbon content of the soil was determined
before waste application. The determination of the % carbon content of the soil (Table 5)
was a necessary step to minimize competition for other carbonaceous substrates, which
could probably lead to preferential utilization by the indigenous microflora. The design of
our experiment did not give insight in the direct fate of the hydrocarbons, if they were
metabolized by the microorganisms and used for growth directly or first cometabolized in
some form. Cometabolism is defined as the metabolism of a compound by a
microorganism, mostly fungi, that the cell cannot use as an energy source or source of
growth (1). The ecological significance of hydrocarbon cometabolism is still uncertain, but
it is known that many microorganisms metabolize aliphatic hydrocarbons that they cannot
use as carbon source for growth, but could serve other microorganisms in some form as a
source of growth. (1). These findings could help to explain the formation of the many
organic intermediates formed in the soiL It is therefore very important to maintain a diverse
microbial consortium in the soil habitat
Using SPC method, the microbial counts recorded in our experiment for both
laboratory mesocosms and field verification studies were relatively high, but generally
reflected the degradation rates. SPC counts from 10^-108 are reported as normal for a good
72
-------
agricultural soil. Of course, this depends on physical and chemical characteristics of the
soils (1). A possible explanation for these recorded high counts, not associated with
substrate utilization, could lie in the fact, that some of the indigenous microbial strains were
spore-forming organisms, that germinated when plated on the petri-dishes. Thus, they
contributed to a higher microbial density on the plate, while, in the natural ecosystem, they
are inactive.Generally, about 50% of a microbial cell consists of carbon, and frequently,
under aerobic conditions, from about 20 to 40 % of the substrate carbon is assimilated, the
rest is released as carbon dioxide.
The general purpose of the commercial inoculum experiment was to study the
effects of a commercial blend of microorganisms on the rates of hydrocarbon degradation.
Since there was no experiment conducted using a sterile soil/waste mixture, inoculated
with the commercial inoculum only, it is not possible to attribute the exact percentages of
microbial transformation to either the commercial or the indigenous microflora. Only
general trends can be observed. The reason for not sterilizing the soil is found in the
purpose for ISBT, namely to simulate the natural ecological environment as closely as
possible. A situation where soil is sterile would never occur in the real ecosystem and was
therefore not considered. Sterilization could also affect the nature of the soil. Since
toxicants often react differently in the presence of one or more other toxicants, no general
behavioral guidelines can be followed and therefore multi-species x multi-toxicant
interactions were the preferred study goal. An artifact in the use of commercially available
inocula may have been, that these inocula are often supplied as freeze dried organisms in a
bran of sawdust containing ample nutrients in the form of fertilizer. During resuspension
with water and application to the assigned soil plots, this fertilizer probably stimulated the
growth of the indigenous microflora just as well as that of the commercial organisms. Due
to the so increased overall biomass, there may be a faster overall initial degradation rate of
the toxicants. No specific and exact statements, however, could be made of what kind of
microorganisms were degrading what part of the waste. Elucidation into the different
73
-------
individual biochemical processes could be achieved, and extrapolations on the
detoxification efficiency of just the commercial cultures could not be made. Therefore,
these results were not useful in explaining the biochemical reactions associated with waste
degradation in the soil environment. The benefit of using commercial inocula consisted of
the enhanced rate of degradation and probably the decreased lag phase. This benefit would
be especially helpful when time is a limiting factor.
The interpretations of the GC data as well as the HPLC data were rendered more
difficult, because all chromatograms showed an increased baseline with time of toxicant
exposure with a substantial amount of unresolved compounds under this baseline. Even
though the current methods of analysis are quite sophisticated, they were not capable of
resolving all the aromatic components into individual compounds. This is partially because
there were thousands or even tens of thousands of molecules present (24). Many of them
were microbial intermediates which are too polar in character to be resoved by the
extraction techniques designed for PAH's. One of the major future goals is to resolve these
unresolved mixtures and try to increase the extraction efficiency and consequently decrease
the amount of residuals. Such investigations are presently under way in our laboratory.
Associated with these interpretations are the aspects of photooxidation
and volatility of the hydrocarbons. The GC chromatograms do not give any evidence of
qualitative toxicant disappearance. The kinetics of toxicant disappearance for lower
molecular weight PAHs, e.g. naphthalene, can not be completely attributed to a microbial
biotransformation scenario, but may also involve the high volatility of these compounds.
Moderate abiotic losses of these PAH's due to volatility have been reported (65), but are
believed to not be substantial. Photooxidation of the PAH's was minimized in the
mesocom tests, by incubating the experimental units, covered, in the dark. Other
unidentified abiotic mechanisms of PAH loss probably occurred Additional investigations
into identifying intermediate abiotic transformation processes are under way. In the field
experimental studies, there was no covering of the experimental plots and the amount of
74
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hydrocarbons lost due to photooxidation could not be determined, but only materials in a
very thin layer after each application and tillage are going to be photooxidized . Therefore,
photooxidation was insignificant in direct sunlight However, a few cm of dark soil may
get hot enough to contribute to excessive volatilization.
In landfarm soil experiments conducted by Bossert et al. (8), analysis of oily
sludge at the conclusion of a 1,280 day laboratory simulation showed F-2 fractions to
have been more rapidly eliminated than F-l fractions. Although parallels can be drawn
with our field experiment, their F-2 fraction constituted only a small portion of the total
hydrocarbons present, 47% of the total hydrocarbons applied (7 applications) was
eliminated in their bioflask study (8). Preliminary data from our field experiments
indicate a net total hydrocarbon removal of 80.34 % for group I and 83.40% for group
II by day 25. The differences in overall microbial performance in the degradation process
of the phase n and phase ffl investigations reported above and those of Bossert et al. (8)
are due to differences in environmental parameters such as temperature, moisture, soil,
microbial diversity/density and other biotic or abiotic factors.
Microorganisms are generally the first biota exposed to the toxic insults by toxicants
in the soil environment Therefore, they are useful for initial toxicity tests since they are
generally well adapted (37). The Microtox™ toxicity test provides a rapid measurement of
the WSF concentrations in a soil sample at the time of sample collection. Changes in the
light output from luminescent bacteria (Photobacterium phosphorewri) are monitored in a
photometer when exposed to various concentrations of toxicants (13). It does not provide a
measure of of the potential accumulation of refractory hazardous organics in the waste-soil
matrix.
The results of the tested field samples indicated limited downward migration of
some organic components within an acceptable range, but generally exhibited patterns of
normal soil functioning (Matthews, personnel communications). This downward leaching
indicated, that the waste reapplications needed to be carefully evaluated in order to prevent
75
-------
overloading of the field plots (Matthews). Also, it must be emphasized, that there were
potential additive, synergistic and antagonistic interactions among the metabolites and
intermediates formed in the soil samples over time. These interactions generally render the
identification of those constituents, that exhibit the toxic effect on the bacteria quite
difficult. However, the test is well suited to serve as a primary screening test to indicate
whether a sample is non-toxic, toxic or very toxic. These results were especially useful if
correlations and comparisons with other bioassays could be done (13). However, a
chemical's toxicity varies dramatically from one species to another, as has been shown by
experiments done by Liu (37), who tested 6 different cultures of bacteria for the same
variety of toxicants (12 test chemicals). Therefore, our results do not give much evidence
in all the interactions between the different bacterial or fungal species in the soil. The
interactions between the biota and the toxicants are very complicated in the ecosystems and
there are no general patterns to follow (37). The studies done by Liu demonstrate the
extreme complexity and unpredictability of the biota-toxicant interactions. In light of these
findings, the focus of the ecotoxicological research done in our experiments was to
determine the overall trends of toxicant disappearence under optimal environmental
parameters. Environmental insults and stresses are thus best monitored in multi-species
multi-toxicant experiments.
Another major problem occured in sample variability. Mixing the soil with the
grease was difficult due to the high viscosity of the sludge. Hence, lack of homogenicity
may have introduced sampling errors. Sample errors were especially problematic in
HPLC analyses, while for the microbial methods, they were compensated by using a
Turrax homogenizer prior to further preparation. Sample variability was smaller in GC
analyses, because composite samples of the replicate mesocosms were analyzed, while
the analyses by HPLC always involved individual samples.
Parallelling the disappearence of some of the aromatics such as phenanthrene, an
increase in their methylated homologs was observed. These methylated homologs are
76
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VI. Conclusions
Microorganisms play a key role in the turnover of carbon in nature. Most of the
known metabolic pathways have been derived from controlled laboratory studies with pure
cultures and single substrates. However, this scenario does not reflect real environmental
conditions, and predictions of microbial behavior under field conditions cannot be made.
Therefore, multiple-toxicant, multiple-species interactions were monitored in our
experiments to evaluate the suitability of the hazardous waste site for ISBT.
Optimal toxicant loading rate windows, determined in screening tests, were shown
to be acceptable for inducing microbial biotransformation/biodegradation in mesocosm
units and minimizing acute leachate toxicity. In the above studies, all of the compounds
analyzed exhibited decreases in concentration over time, for both laboratory and field tests.
The decreases were mostly attributed to microbial activity by the indigenous soil
microfiora. However, undefined abiotic losses were noted and need to be further studied.
Both waste types, the lagoon and buried wastes, at both loading rates, 2.5% and 4%, were
degraded by the indigenous microfiora. Mutagenicity and carcinogenicity of these
intermediate degradation products need to be evaluated.
Task n and task HI studies verified microbial degradation and detoxification
processes. It was shown that the indigenous microfiora was adapted to the wastes and
capable of degrading it Substantial hydrocarbon degradation was observed, even though
not all of it could be attributed to microbial activity, as has been discussed earlier.
In short, it can-be concluded, that:
* group two (PL4, PL5) plots show indication of waste-overloading toward the end
of the applications. Microtox™ and plant biomass analyses confirmed these results.
* soil composition of plots was too high in silt-clay content, causing waterlogging
and limiting aeration. Soil mixtures for future applications should contain higher
sand contents and less clay contents thus preventing the hydrocarbons from binding
to the clay and reducing waterlogging, but the sand content should be low enough
78
-------
to not promote leaching of soluble compounds. LSU investigators are willing to
confer with IT Corporation engineers on a final soil/sand mixture at such time in
which a general contractor for this project is named (see further recommendations
on hydraulic overloading).
Waste composition used in field plots was not exactly similiar to the wastes tested
in the laboratory (confirmed by GC/MS). This may be due to collection from a
different location at the site thus rendering GC data comparisons more difficult.
A complete GC/ MS profile of hazardous wastes prior to waste application is
mandatory. This will facilitate subsequent post treatment analysis of each waste
application.
Microtox results of WSF toxicity generally exhibited patterns representative of
normal operation of natural soil treatment processes (Matthews), but limited
downward migration of organic constituents has been observed, however no
deeper man the acceptable treatment zone.
Microtox™ data suggested, that time periods between sequential reloadings need to
be carefully evaluated and adjusted according to environmental parameters with
special respect to downward leaching of organic constituents.
All compounds analyzed exhibited decreases in concentration over time, but proper
identification and monitoring of potential toxic intermediates formed by the
microbial consortium need to be further evaluated.
Indications from IT Corporation engineers, that hydraulic overloading of lagoon
wastes due to excessive amounts of water not separated from the sludge were
recently presented to LSU investigators. This variable, although not mentioned in
previous correspondence represents a significant problem for general waste
application. It is of the same order of magnitude as waste over loading.
79
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It is recommended that IT Corporation suggest specific engineering improvements
for waste application so as to minimize anaerobic conditions during waste
application due to hydraulic over loading (phase separation of oil and water).
* Laboratory data indicated that soil mixtures were adaequate from a microbiological
perspective. Field tests, based on GC/MS and TOC data, indicated that this mixture
was adaequate, i. e. minimal leachate problems. However, a more complete mixing
of waste to soil is needed.
Heavy equipment, specifically modified for waste application efforts is currently
available. IT Corporation has been advised of potential manufacturers of this
equipment
* For large scale waste application, significant quantities of soil'exhibiting minimal
microbial activity (specifically microbial activity related to hydrocarbon
degradation) may be used.
It is recommended that either adapted microbial populations be generated in these
soils prior to the initial waste application or commercial inocula be used
Under proper management microbial degradation and detoxification of the site is
scientifically verifiable and economically feasable. Post-closure monitoring of
soil and leachate collected from the site is recommended for a time period of 30
years after setup of landtreatment facilities. Closure activities are varied throughout the site
and include more than just biodegradation. Although we have been requested to provide
engineering input on many of these activities, our focus has been strictly associated with
biological closure, L e. the degradation of the waste materials. We choose not to comment
on other aspects of the Phase in Engineering Design Final Report These activities are quite
properly the responsibility of IT Corporation.
80
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believed to be formed by microbial activity. Methyl groups are often converted to
carboxyl groups prior to ring cleavage. In certain compounds, however, the methyl is
not removed before the ring is opened (11). The carboxyl is often, but not always,
removed before ring cleavage. The methoxyl is replaced by a hydroxyl and gives rise to
formaldehyde (1).
77
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BIBLIOGRAPHY
[1] Alexander, M. 1977. Introduction to Soil Microbiology. John Wiley & Sons. N.Y.
Chichester, Brisbane, Toronto. 115-225
[2] Alexander, M. 1981. Biodegradation of Chemicals of Environmental Concern.
Science, Vol. 211, 9. Jan. 1981. 132-139
[3] Alexander, M. 1985. Biodegradation of Organic Chemicals. ES&T Vol.
18, No. 2, 106-110.
[4] Anderson, J.P.E. and Domsch, K.H. 1978. A Physiological Method for the
Quantitative Measurement of Microbial Biomass in Soils. SoilBiol.
Biochem. Vol 10. 215-221.
[5] Atlas, R.M., Boehm, P.D. and Calder, J.A. 1981. Chemical and Biological
Weathering of Oil from the Amoco Cadiz Oil Spillage in the Littoral
Zone. Estuarine, Coastal and Shelf Science 12:589-606.
[6] Bianchini, M., R. Portier, K. Fugisaki and C. Henry. 1986. Determination of
Optimal Toxicant Loading for Biological Closure of A Hazardous Waste Site. •
Manuscript in press, ASTM Proceedings 1986.
[7] Bois, R, M. Vaillant and P. Vasseur. 1986. Multiple Regression Analysis of
Toxic Interactions: Application to the Microtox™ Test and General Comments.
Bull Environm. Contain. Toxicol. 1986. 36: 707-714. Springer Verlag New York
Inc. December 1978.56-558 and 101-107.
[8] Bossert, I., Kachel,W.M. and Bartha, R. 1984. Fate of Hydrocarbons
during Oily Sludge Disposal in Soil. Applied and Environmental
Microbiology, April 1984. 763-767.
[9] Bossert, I. and Bartha, R. 1984. The fate of Petroleum in Soil Ecosystems in:
Petroleum Microbiology, edited by R.M. Adas. Macmillan Publishing Company
N.Y., London. 435-475
[10] Casarett and Doull, J. 1980. Toxicology. The Basic Science of Poisons. Sec.
edition, edited by J. Doull; CD. Klaassen and M.O. Amdur. Macmillan
Publishing Co., Inc. New York. 91-93 and 632-661.
[11] Cemiglia, CE. 1983. Microbial Metabolism of 4-, 7-, 10-,
Methylbenz[a]anthracenes. in: Cooke, Marcus and Dennis, Anthony J. 1983.
Polynuclear Aromatic Hydrocarbons: Formation, Metabolism and Measurement
Battelle Press, Columbus, Richland 283-293
[12] Cemiglia, CJE. 1984. Microbial Transformation of Aromatic Hydrocarbons, in:
Petroleum Microbiology, edited by RM. Adas. Macmillan Publishing Company
N.Y., London. 99-129
81
-------
[13] Coleman, R.N. and A.A. Qureshi 1985. Microtox® and Spirillum voluntans Tests
for Assessing Toxicity of Environmental Samples. Bull. Environm. Comam.
Toxicol. 1985. 35: 443-451. Springer Verlag New York Inc.
[14] Connell, D.W. and Miller, GJ. 1984. Chemistry and Ecotoxicology of Pollution
John Wiley and Sons, New York, Chichester, Brisbane, Toronto, Singapore. 228-
250.
[15] Colwell, R.R., Brayton, P.R., Grimes, D.J., Roszak, D.B., Huq, S.A. and
Palmer, L.M. 1985. Viable, but non Culturable Vibrio Cholerae and
Related Pathogens in the Environment: Implications for Release of
Genetically Engineered Microorganisms. Bio/Technology Vol. 3, Sept
1985 817-820.
[ 16] D'Appolonia/GDC 1983. Draft Report-Vol. I. Remedial Investigation phase one-
Element I, Contract No 28135-83-01. Old Inger abandoned hazardous waste site,
Darrow, LA. Louisiana Department of Natural Resources, Baton Rouge, LA. DCE
Project No. SW 83-1124, GDC Project No 83-521. Oct. 1983.
[17] D1 Appolonia/GDC 1984. Draft Final Report Phase H Feasibility Study. Old Inger
abandoned hazardous waste site, Darrow, LA. Louisiana Department of
Environmental Quality, Baton Rouge, LA. D'Appolonia Project No. 84-6058.
GDC Project No. 84-501. Contract No. 28800-84-01. May 1984.
[18] Dipple, Anthony. 1985. Polycyclic Aromatic Hydrocarbon Carcinogenesis: An
Introduction, in: Harvey, R.G. Polycyclic Hydrocarbons and Carcinogenesis.
ACS Symposium Series 283. 1-19
[19] EPA/530-SW-84-015 Dec. 1984. Permit Guidance Manual on
Hazardous Waste Land Treatment Demonstrations.
[20] EPA-600/8-78-017. Dec.1978. Microbiological Methods For Monitoring the
Environment Water and Wastes. 32-59 and 101-108.
[21] Farrington, J.W., et al. 1982. Petroleum Contamination: Quantification
and Passive Tagging in Organisms and Sediments. U.S.
Department of Commerce, NTIS, March 1982, PB 82-254087
[22] Federal Register/ VoL 49 No. 209. Oct. 1984/ Rules and Regulations,
Method 610,533-541.
[23] Fedorak, P.M: and D.W.S. Wesflake 1981. Degradation of Aromatics and
Saturates in Crude Oil by Soil Enrichments. Water, Air, and Soil Pollution 16.367-
375.
[24] Gibson, D.T. Microbial Metabolism, in: Hutzinger, 0.1980. The Handbook
of Environmental Chemistry VoL 2 Part A, Springer-Verlag Berlin Heidelberg
New York Tokyo 1980. 161-189 and 231-244.
[25] Harvey, R.G. 1985. Synthesis of Dihydrpdiol and Diol Epoxidc Metabolites of
Carcinogenic Polycyclic Hydrocarbons.in: Harvey, R.G. Polycyclic Hydrocarbons
and Carcinogenesis. 1985. ACS Symposium Series 283.19-35.
82
-------
[26] Herbes, S.E. and L.R. Schwall. 1978. Microbial Transfonnation of Polycyclic
Aromatic Hydrocarbons in Pristine and Petroleum- Contaminated Sediments.
Applied and Environmental Microbiology, Feb. 1978. 306-316.
[27] Hill, E.G. Biodegradation of Petroleum Products, in: Petroleum Microbiology.
edited by R. M. Atlas. Macmillan Publishing Company N.Y., London. 579-619.
[28] Holm.-Hansen, O. and Booth, C.R. Limnology and Oceanography,
Vol.11, 1966, 510-519.
[29] Hutzinger, 0.1982. The Handbook of Environmental Chemistry Vol. 3
Part B, Springer-Verlag Berlin Heidelberg New York Tokyo 1982. 117-147.
[30] Hutzinger, O. 1985. The Handbook of Environmental Chemistry Vol.2
Part C, Springer-Verlag Berlin Heidelberg New York Tokyo 1985.
[31] Jensen, H.Z., Soil science, Vol. 30, 1930, 59-77
[32] Johnston, J.B. and Robinson, S.G. 1984. Genetic Engineering and
the Development of new Pollution Control Technologies. U.S.
DepL of Commerce National Technical Information service. PE 84-
148972; EPA-600/2-84-0337.
[33] Karcher, W., RJ. Fordham, JJ. Dubois, P.G.J.M. Claude, J.A.M. Ligthart
1985. Spectral Adas of Polycyclic Aromatic Compounds including data on
occurence and biological activity. D. Reidel Publishing Company
Dordrecht/Boston/Lancaster for the European Communities. 30-109.
[34] Karl, D.M., Microbiology Reviews, Vol. 44, 1980. 739-796.
[35] Korte, F. 1985. Concepts for the Ecptoxicological Evaluation of Chemicals:
Ecotoxicological Profile Analysis, in: Niirnberg, H.W. 1985. Pollutants and their
Ecotoxicological Significance. John Wiley & Sons, Chichester, New York,
Brisbane, Toronto, Singapore 337-363.
[36] Lehr, R.E., S. Kumar, W. Levin, A.W. Wood, R.L. Chang, A. H. Conney,
KYagi, J. M. Sayer and D.M Jerina. 1985. The Bay Region Theory of
Polycyclic Aromatic Hydrocarbon Carcinogenesis. in: Harvey, R.G. Polycyclic
Hydrocarbons and Carcinogenesis. 1985. ACS Symposium Series 283. 63-85.
[37] Liu, D. 1985. Effect of Bacterial Cultures on Microbial Toxicity Assessment. Bull.
Environm. Contain. Toxicol. 1985.34: 331-339. Springer Verlag New York TJIC.
[38] London, S.A., C.R. Mantel and J.D. Robinson 1984. Microbial Growth Effects
of Petroleum and Shale-derived Fuels. Bull. Environm. Contain. Toxicol. 1984.
32: 602-612. Springer Verlag New York me.
[39] Mandelstam, J. and K. McQuillen 1982. Biochemistry of Bacterial Growth, Third
edition, Blackwell Scientific Publications, Oxford, London, Edinburgh, 142-158
83
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[40] Martel, L. M., J. Gagnon, R. Mass6, A. Leclerc and L. Tremblay. 1986.
Polycyclic Aromatic Hydrocarbons in Sediments from the Saguenay Fjord, Canada
Bull. Environm. Contain. Toxicol. 1986. 37: 133-140. Springer Verlag New York
Inc.
[41] Martin, J.P., So/7 science, Vol. 69, 1950, 215-233.
[42] Meyer, J.S., Marcus, M.D., Bergman, H.L. 1984. Inhibitory Interactions
of Aromatic Organics During Microbial Degradation. Environmental Toxicology
and Chemistry, Vol. 3, 583-587.
[43] Morgan, D. and Monmaney, T. 1985. The Bug Catalog. Science 85
July/August 37-41.
[44] Miiller, P. 1985. Ecological Parameters and their Significance for Pollution
Monitoring in the Terrestrial Environment in: Niirnberg, H.W. 1985. Pollutants
and their Ecotoxicological Significance. John Wiley & Sons, Chichester, New
York, Brisbane, Toronto, Singapore 291 - 317
[45] Nannipieri, P., R.L Johnson and E.A. Paul, 1978. Criteria for
Measurement of Microbial Growth and Activity in Soil. Soil Biol. Biochem.
Vol. 10. 223-229.
[46] Neff, Jerry M. 1979. Polycyclic Aromatic Hydrocarbons in the Aquatic
Environment Sources, Fates and Biological Effects. Applied Science Publishers
LTD, London. 61-73 and 102-149.
[47] Norton, Patricia, Louisiana Department of Environmental Quality, Report, March
11, 1985. 12.
[48] Office of the Governor. 1984. Environment and Health in
Louisiana: The Cancer Problem, Louisiana State Planning Office, Baton
Rouge, La.
[49] Overton, E.B., L.V. McCarthy, S.W. Mascarella, M.A.
Maberry, S.R. Antoine and JJL Laseter. Detailed Chemical
Analysis of Ixtoc-I Crude Oil and Selected Environmental Samples
from the Research and Pierce Cruises, in: Proceedings of a
Symposium on Preliminary Results from the September 1979
Researcher/Pierce Ixtoc-I Cruise Dec. 1980. U.S. Department of
Commerce, National Oceanic and Atmospheric Administration,
Office of Marine Pollution Assessment 439-455.
[50] Pfaender F.K. and E.N. Buckley UI1984. Effects of Petroleum on Communities.
in: Petroleum Microbiology, edited by R.M. Atlas. Macmillan Publishing Company
N.Y., London. 507-537
84
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[51] Portier, RJ. 1985. Comparison of Environmental Effect and
Biotransformation of Toxicants on Laboratory Microcosm and Field
Microbial Communities, Validation and Predictability of
Laboratory Methods for Assessing the Fate and Effects of
Contaminants in Aquatic Ecosystems, ASTM STP 865, T.P.
Boyle, Ed., American Society for Testing Materials, Philadelphia, 1985
14-30.
[52] Portier, RJ. and S.P. Meyers. 1982. Monitoring Biotransformation and
Biodegradation of Xenobiotics in Simulated Aquatic Microenvironmental Systems.
Developments in Industrial Microbiology, Vol. 23,1982 459-475.
[53] Portier, RJ., H.M. Chen and S.P. Meyers. 1983. Environmental Effect
and Fate of Selected Phenols in Aquatic Ecosystems Using Microcosm
Approaches. Developments in Industrial Microbiology, Vol. 24,
1983. 409-424.
[54] Powledge, T.M. 1983. Prospects for Pollution Control with Microbes.
Bio/Technology Nov. 1983. 743- 755.
[55] Riggin, R., P. Strap and S. Billets. 1983. Development of a Screening Method for
the Determination of Total Polynuclear Aromatic Hydrocarbons (PAH) in Water
and Wastewater. in: Cooke, Marcus and Dennis, Anthony J. 1983. Polynuclear
Aromatic Hydrocarbons: Formation, Metabolism and Measurement Battelle Press,
Columbus, Richland 1021-1035.
[56] Schniirer, J. and Ross wall, T. 1982. Fluorescein Diacetate Hydrolysis as
a Measure of Total Microbial Activity in Soil and Litter. Applied and
Environmental Microbiology, June 1982.1256-1261.
[57] Sisler, F.D. and C.E. ZoBell. 1947. Microbial Utilization of Carcinogenic
Hydrocarbons. Science, Nov. 28, 1947. 521-522.
[58] Southworth, GJR. and Keller, J.L. 1984. Partitioning of Naphtalene,
Methylnaphtalenes and Biphenyl between Wastewater Treatment
Sludges and Water. Environmental Toxicology and Chemistry, Vol. 3. 575-
582.
[59] Tabatabai, M.A. and Bremner, J.M. 1969. Soil Biology and
Biochemistry Vol. 1,1969, 301-307.
[60] Traynham, J.C. 1980. Organic Nomenclature: A Programed Introduction.
Prentice-Hall, INC., Englewood Cliffs, New Jersey 07632.41-51 and 66-74.
[61] Van de Werf, R. and Verstraete, L.M.J. 1979. in: Proceedings, International
Symposium Analytical Applications of Bioluminescence and
Chemiluminescence, Schram et al. Eds., 1979 333-338.
[62] Verstraete, LJvU., K. De Coninck and K. Vlassak, 1983. ATP content of soils
estimated by two contrasting extraction methods. Soil/Biol. Biochem, Vol. 15,
No.4 397-402.
85
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[63] Vestal, J.R., JJ. Copney, S. Crow, and J. Berger. 1984. Effects of
Hydrocarbons on Microorganisms, in: Petroleum Microbiology, edited by R.M.
Atlas. Macmillan Publishing Company N.Y., London 475-507.
[64] Wilson, J.T., J.F. McNabb, D.L. Balkwill, and W.C. Ghiorse 1983.
Enumeration and Characterization of Bacteria Indigenous to a Shallow Water
Table Aquifer. Ground Water, Vol. 21 No 2, March-April 1983, 134-142.
[65] Zander, M. 1980. Polycyclic Aromatic and Heteroaromatic Hydrocarbons, in:
Hutzinger, O.I980. The Handbook of Environmental Chemistry Vol.3
Part A, Springer-Verlag Berlin Heidelberg New York Tokyo 1980. 109-128.
86
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APPENDIX A
Literature Review on Hydrocarbon Biodcgradation
A. General
Microbial metabolism of hydrocarbons has been reported in the literature for several
decades. Some of the first investigations date back as early as 1928 , when Gray and
Thornton (46) first reported soil bacteria capable of decomposing certain aromatic
compounds. In 1941, Bushnell and Haas (46) documented microbial degradation of
certain hydrocarbons. Sisler and Zobell (57), in 1947 used microorganisms of marine
origin in their experiments to degrade aromatic hydrocarbons. They studied the utilization
of polyaromatic hydrocarbons (PAH's) by mixed cultures of marine bacteria. PAH's were
introduced into seawater cultures adsorbed to ignited sand. The amount of PAH
metabolized by the bacteria was determined by measuring the amount of carbon dioxide
evolved in hydrocarbon oxidation and substracting carbon dioxide produced by the control
cultures. In these experiments, phenanthrene and anthracene were metabolized more
rapidly than naphthalene, benz(a)anthracene, and dibenz(a,h)anthracene. (57).
In the present decade, it is well known that hydrocarbons are ubiquitous in the
environment and even found in relatively pristine areas. Their sources are of natural as well
as anthropogenic origin. Due to the toxicity, mutagenicity and carcinogenicity that many of
them exhibit after undergoing metabolic activation, hydrocarbons in the environment may
pose a hazard to the biota and, ultimately, to human health (12). Major environmental fate/
transport mechanisms include:
evaporation (volatilization)
photochemical oxidation
sedimentation
microbial degradation
87
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1. Definition
Hydrocarbons are compounds containing carbon and hydrogen.
Aliphatic hydrocarbons are straight or branched chain hydrocarbons of various lengths.
Aliphatic hydrocarbons are contained naturally in waxes and other constituents of plant
tissues as well as in petroleum or petroleum products. Their transformations are
therefore of great significance in the terrestrial carbon cycle (1). The rate of their
decomposition is markedly affected by the length of the hydrocarbon chain (1).
Aromatic hydrocarbons contain the benzene ring as the parent hydrocarbon.
Several benzene rings joined together at two or more ring carbons form PAH's. The
toxicity of these molecules is determined by the arrangement and configuration of the
benzene rings. The hydrogens in the aromatic hydrocarbons may or may not be
substituted by a variety of groups. Some of the common substituents are -Cl, chloro; -
Br, bromo; -I, iodo; -NO2, nitro; -NO, nitroso; and -CN, cyano (60).
2. Sources and Formation
Most of the aromatic hydrocarbons are initially formed by the pyrolysis of
organic material (24). In this process, the temperature determines the type of compound
formed. For example, unsubstituted PAH's are formed at high temperatures (2,000 C)
whereas alkyl-substituted molecules predominate at 80-150 C. The latter temperature
range is usually associated with the formation of petroleum (24). Generally, PAH's are
formed when organic material containing carbon and hydrogen is subjected to
temperatures exceeding 700 C, which is the case hi pyrolytic processes and with
incomplete combustion (65). Some common sources associated with incomplete
combustion are cigarette smoke, automobile exhaust, and industiral processes.
The higher the number of joined benzene rings, the lower the rate of degradation.
The very high molecular weight PAH's are less significant in environmental pollution
problems, due to their low volatility and solubility (65). The growth rates of bacteria on
PAH's are directly related to the solubilities of the PAH's (65). Solubility and
88
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The mechanisms used by bacteria for the introduction of hydroxyl moieties into
PAH's will depend on whether the substrate contains alkyl substituents (24). The initial
step of aromatic metabolism consists of the modification or removal of substituents on
the benzene rings and the introduction of hydroxyl groups (2). The first metabolites of
unsubstituted PAH's created by bacteria are cis-dihydrodiols, formed by the
incorporation of two atoms of molecular oxygen. Fungi, in contrast, form trans-
dihydrodiols. The enzymes catalyzing these processes are oxygenases, known as
cytochrome P450 enzyme complexes. Bacteria use dioxygenases, a multicomponent
enzyme system consisting of a flavoprotein, an iron-sulfur protein, and a ferredoxin
(12).
Although, initial phases of the degradation pathways differ, the reactions proceed
such that only a few common and key intermediates are produced. These few are then
metabolized by essentially similiar processes. Most common of these intermediates are
catechol, protocatechuic acid and to a lesser degree, gentisic acid (1) (Fig. 2). These
three molecules have in common the presence of two hydroxyls. They are then further
oxidized by the five pathways also shown in Fig.2. The products of these reactions,
namely pyruvate, fumarate, and succinate may then be incorporated in the TCA and other
biochemical cycles (39). The degradation pathways involved are dictated by the site of
cleavage of the aromatic nucleus.
Naphthalene and its alkylated homologs are among the most water-soluble and
potentially toxic compounds in petroleum. Fig.3 depicts the pathway for the bacterial
oxidation of naphthalene to catechol. It also shows the different pathways for the
bacterial oxidation of phenanthrene. Oxidation of this compound by fungi has not been
reported. Special interest has been paid by various researchers to the degradation of
anthracene and its derivatives. These compounds are not acutely toxic, but possess a
structure also found in other carcinogenic PAH's (12). Degradation of anthracene has
-------
adsorbtivity are the most important physical properties that influence the rate of
transformation. Among the chemical properties, photochemical reactivity is the most
relevant Tricyclic or larger PAH and related heterocyclic systems show a very reactive
photochemical behavior. They have strong UV adsorption at wavelengths longer than
300 nm (present in solar radiation) and most are readily photooxidized. Photooxidation
plays one of the major roles in the removal of PAH's from the environment (29).
Adsorbed PAH's are photooxidized more rapidly than dissolved PAH's. (65).
The chemical structures of some of the major aromatic hydrocarbons are shown
in Fig.l. Biological activity of these compounds depends on their inherent
stereochemistry. The addition of another benzene ring in a select position of the
compound can result in the formation of a powerful carcinogen, even if the parent
compound does not exhibit much toxicity (10). The reactive sites of the molecules are
called "Bay- regions" (10). Such a Bay region is found in phenanthrene, the simplest
PAH. It resembles that of benz(a)-anthracene and benz(a)pyrene, and is the region
between an angular benzo ring and the rest of the molecule (11). If dihydrodiol-epoxides
are formed in this region, the molecule becomes very biologically reactive and is
suspected to be a ultimate carcinogen. The primary active carcinogen is usually in the
form of a diol epoxide (25). Phenanthrene itself has been shown to be inactive or only
slightly mutagenic in Salmonella assays, but its metabolites may be highly mutagenic and
tumorigenic(ll).
Historically, it was believed, that a certain area, called the "K region" was related
specifically to the carcinogenic potential of a hydrocarbon compound. Evidence now
suggests mat activation of PAH's is not likely associated with this K region, but rather
occurs via a two step oxidation with the eventual formation of dihydrodiol epoxide (10).
Another portion of the molecule, called the "L region" can increase the carcinogenic
potency of the molecule, if there are substituents on these positions (i.e., the 7 and 12
90
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Of these, microbial degradation is the area most extensively studied and
commercialized as evidenced by the most recent developments in biotechnology and
genetic engineering. It is a major mechanism for compound removal from sediments and
terrestrial systems. Microbial degradation of aromatic hydrocarbons by bacteria as well
as fungi has been documented in numerous publications. The degradation processes are
generally inversely proportional to the ring size of the respective PAH molecule. The
lower weight PAH's are degraded more rapidly, while molecules with more than three
condensed rings generally do not serve as amenable substrates for microbial growth
(24). Hence the effectiveness of creosote as a wood preservative.
B. Aromatic and Aliphatic Hydrocarbons
The capacity of microorganisms to grow in a given habitat is determined by their
ability to utilize the nutrients in their surroundings (1). Among the energy sources
available to be utilized by soil heterotrophic microorganisms are cellulose, hemicellulose,
lignin, starch, chitin, sugars, proteins, hydrocarbons and various other compounds (1).
Numerous hydrocarbons, or their derivatives, are naturally synthesized within the soil
while others are added to the soil from various pollution sources. Their mineralization
and formation by the indigenous microfiora are a fundamental component in the general
carbon cycle (2).
The three major types of microbial metabolism are: fermentation, aerobic
respiration and anaerobic respiration (24). Aerobic respiration plays the most important
role in the transformation of PAH's. Very little anaerobic respiration of PAH's has been
reported. However, anaerobic biodegradation of PAH's has been observed where suited
electron acceptors were supplied (2). Aerobic respiration initially involves the
incorporation of molecular oxygen in the hydrocarbon molecules. The hydrocarbons are
then converted to more oxidized products. Energy produced during these oxidation
processes is partially used in the synthesis of protoplasmic constituents (24).
91
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carbons in benz(a) anthracene) (10). These structure-activity relationships and the
locations of the different regions are shown in Fig.l.
C. Microbial Metabolism of Hydrocarbons
There are various and controversial scenarios reported in the literature as to the
physical form under which the hydrocarbons are metabolized. Some studies indicate the
presence of large hydrocarbon droplets, others mention micro-drops as small or smaller
than the microbial cells, still others suggest the importance of the water soluble fraction
(WSF) or the utilization of the hydrocarbons in a vapor phase (38). There are also
reports on the importance of emulsifying agents for initiating hydrocarbon utilization.
However, most reported microbial hydrocarbon metabolism processes are intracellular
oxidation processes (38).
Historically, most of the investigations of PAH biodegradation were concerned
with measuring the amount of CC>2 produced or the fractions of the toxicants (parent
molecule) converted into CO2. In these early studies, CO2 production was the major
focus of attention with little consideration paid to the intermediates formed. Only recently
has it been recognized that there is a need to investigate these metabolites and the ratio of
polar compounds to CO^ The oxygenated polar compounds may be highly mutagenic
and/or accumulative in the aquatic/terrestrial environment and thus be dangerous to living
cells. Recent advances in analytical techniques (such as Thin Layer Chromatography
and/or MS) have revealed the subtle complexity of biotransformation intermediates and
endproducts.
1. Bacterial Transformation (Biotransformation)
Bacteria are the dominant group involved in the degradation of PAHs. The most
widely occuring species are Pseudomonas, Myobacterium, Acinetobacter, Arthrobacter,
Bacillus andNocardia (1). Bacteria can oxidize PAH'S ranging from the size of benzene
to benzo(a)pyrene. For more highly condensed PAH'S, there is little evidence of
bacterial oxidation (24).
92
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been reported by bacteria as well as fungi and follows the general degradation pattern of
the other PAH's.
2. Fungal Transformation (Biotransformation)
Many fungi cannot grow with PAH's as a sole source of carbon and energy, but
still have the ability to oxidize these compounds (24). Fungi carry out reactions similar
to mammals in the degradation process. Therefore, fungi are often used as model
systems. Their enzyme systems for the oxidation of PAH's differs from that of bacteria
(e.g. mono-oxygenases) and is similiar to that of higher organisms. The cytochrome
P450 mono-oxygenase system catalyzes the initial steps in the oxidation of these
lipophilic PAH's. Many fungi add hydroxyls to the ring structures without being able to
open the ring, but subsequent ring opening and cleavage of ether bonds can then be
brought about through cometabolic conversions (1). Cometabolism is defined as the'
metabolism of a compound by a microorganism that the cell is unable to use as an energy
source or source of growth (1).
An example for a fungal metabolic pathway quite similiar to those in mammalian
systems for the oxidation of naphthalene is given in Fig.4. In contrast to bacteria, fungi
incorporate only one atom of molecular oxygen into naphthalene via a cytochrome P450
mono-oxygenase (12). ^^
93
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APPENDIX A
Fig. 1: Structure-activity relationships of PAH's
Fig. 2: Common intermediates in bacterial oxidation of PAH's
Fig. 3: Pathway for the bacterial oxidation of naphtalene to catechol
Fig. 4: Pathway for the fungal oxidation of napthalene
Fig. 5: Pathway for the bacterial oxidation of phenanthrene
Fig. 6: Old Inger Site Map
94
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Fig. 1: Structure-activity relationships of PAH's
source: Casarett and Doull, J. 1980. Toxicology. The Basic Science of Poisons.
Sec. edition, edited by J. Doull; C.D. Klaassen and M.O. Amdur.
Macmillan Publishing Co., Inc. New York. 92.
EXAMPLES OF CARCINOGENIC POLYCYCLIC
OR HETEROCYCLIC AROMATIC HYDROCARBONS .
Bay region
region
Benz[a]anthracene; R » H
7,12-DimethylbenzManthracene;
R-CH,
Benzo[a]pyrene
L region
Dibenz[fl,A]anthracene
H,C
3-MethylchoUnthrcne
Dibenz[a, AJacridtne
95
-------
Fig. 2: Common intermediates in bacterial oxidation of PAH's
source: Alexander, M. 1977. Introduction to Soil Microbiology. John Wiley &
Sons. N.Y. Chichester, Brisbane, Toronto. 219.
HOOC.
OH
-OH
-OH
COOH
Catechol
Protocatechuic
acid
H
Gentisic
acid
96
-------
Fig. 2: Common intermediates in bacterial oxidation of PAH's
source: Alexander, M. 1977. Introduction to Soil Microbiology. John Wiley &
Sons. N.Y. Chichester, Brisbane, Toronto. 219.
HOO
HOOC
-2U
COOH
|
(
"• nnw <
^COOH or
Muconic acid
:H
:H
fH v
H\
COOH
COOH
CH „„
1 -"X
HOOC-C /
500H nr CH
or ••
;OOH
CH
V
COOH 1
\CH, )
1 s
CO
i
CH,
Xin,
COOH ,
Acetic
acid
COOH
, ^TH^
COOH
1
CH,
' 'CH,
i
COOH
Succinic
acid
Protocatechuic
acid
COOH
0-Carboxymuconic acid
CHO
HOO
Protocatechuic
acid
>H CH
COH
COOH
2-Hydroxymuconic tcmialdehyde
COOH
COOH
io
^•»-—— i -— * CWi
:HO HOOC-C T „
^.HHOOC-60H
CHO
4-Carboxy-2-hydroxyrnucociic
COOH
Pyruvic acid
Pyruvic acid
100H
CH,
semialdehyde
COOH
CH
OH {
OCOOH i
-s
COOH
CH
Fumaric
acid
COOH
}CH,
-io
COOH
Maleylpyruvic acid
Pyruvic
acid
97
-------
Fig. 3: Pathway for the bacterial oxidation of naphtalene to catechol
source: Cerniglia, C.E. 1984. Microbial Transformation of Aromatic
Hydrocarbons, in Petroleum Microbiology, edited by R.M. Atlas.
Macmillan Publishing Company N.Y., London. 107
H
(+)-cis-l,2-Dihydroxy-l,2-dihydronaphtha!ene
1,2-Dihydroxynaphthalene
cU-o-Hydroxy benzol pyruvic acid
I
OH
Salicyloldehyde
>H
Salicylic acid
'OH
Catechol
META PATHWAY
ORTHO PATHWAY
98
-------
Fig.4: Pathway for the fungal oxidation of napthalene
source: Cemiglla, C.E. 1984. Microbial Transformation of Aromatic
Hydrocarbons. in:Petrolium Microbiology, edited by R.M. Atlas.
Macmillan Publishing Company N.Y., London. 110.
... „<
"
$0. *' 'Epoxidi
2.Naphlhol
"OM
Nophihol.n. Nophihol.n* (+MS,2S-dihydr|oxy-l,2.dihydronophlhoUi»»
1,2-oxid* |M*
OH
•on
l-Nophlhol 2-Nophlhcl
•OM
oiojsscoi j n==
Naphlhalin* NaphlhoUn* . . . .• ...... . ,
12-oxid« (+)-lS.2S-dihydf|o«y.l,2.dihydronapthol«n«
l-Nophlhol 2-Naphlhol
99
-------
Fig.5: Pathway for the bacterial oxidation of phenanthrene
source: Cemiglia, C.E. 1984. Microbial Transformation of Aromatic
Hydrocarbons. in:Petrolium Microbiology, edited by R.M. Atlas.
Macmillan Publishing Company N.Y., London. 112.
100
-------
Fig. 6: Old Inger Site Map
source: D' Appolonia/GDC 1984. Draft Final Report Phase H Feasibility Study.
Old Inger abandoned hazardous waste site, Darrow, LA. Louisiana
Department of Environmental Quality, Baton Rouge, LA. D'Appolonia
Project No. 84-6058 GDC Project No. 84-501. Contract No. 28800-84-01.
May 1984, fig. 3-12.
101
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o
KJ
LAWOH
'•' .''\ ' ' • '.'•'".' ;:. MISSISSIPPI mven : •••.'• : '• '••'•'••; Vj '.. ,-"•'. •;;
•' • fiow ^
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-------
APPENDIX B
Product data by Microbe Masters, Inc.
103
-------
UMJIKSlfii©
MASTERS, Inc
'Biotechnology In Action'
PROCULT DATA
MICRO PRO DEIDXIFCER SERIES
Micro Pro Mutant Bacterial Cultures
Hazardous Waste Treatment
Micro Pro Detoxifier Series is a special blend of bacterial cultures which were selected and
nutated for hazardous waste sites and land farming. This product line has performed successfully
in numerous hazardous waste applications to degrade compounds such as styrene, phenols,
polynuclear arcmatics, amines, ammonia, sulfides, alcohols, and oils and'grease. The product
series has proven itself in actual field trials as an economical tool which can be used to attain
efficient hazardous waste control.
FORM: Dry or liquid bacterial cultures
PHYSICAL PROPERTIES:
Dry Cultures
Appearance
Odor
Bacteria Count
Specific Gravity
pH
Effective Temperature 50-110° F
Shelf Life 1 year
Moisture 15%
DOSAGES:
Tan, free flowing powder
Yeast-like
8-10 billion/gram
0.6
6.0 - 8.5
Liquid Cultures'-
Slightly turbid liquid
Faint grape-lite odor
100 million/ml (nun)
1.0
Neutral
50-110° F
6 months
N/A
Dosages for the products will vary according to the specific situation and also the particular
goals of the evaluation. Typical applications would consist of one or two pounds of dry product
per 100 square feet of waste site.
Please refer to the reverse side for application instructions.
104
11814 Coursey Blvd. Suite 285, Baton Rouge, LA 7^°<6 (504)
-------
SOIL fflOAUCMLNTATION
To ensure proper biodegrarhtjon, ,1 ccmpetible product .can be rcconttsnclej by the tschrjcal
department; or if so desired, a lab screening, or plot study, can be performed on the particular
soil in question.
Initial chemical concentrations should be found and napped for the site.
Selected product should be applied at the rate of 1 Ib./lOO square feet of surface area. This
dosage is effective to about 6-12 inches deep, depending on soil type.
Product should be spread evenly and the entire area thoroughly watered. The site should be kept
damp (standing veter is not recommended) by periodic waterings.
Weekly analyses for ammonia nitrogen, orthophosphate and pH should be performed. If nutrient
levels fall below 5 -ppm, they can be supplemented by using 3-5 Ibs. of commercial fertilizer
(8-6-8, 10-10-10, etc.) per 100 feet.
The above dosage procedure should be applied weekly for two applications and biweekly thereafter
as needed to detoxify the area.
Depending upon the soil penrability, clay content, etc., periodic tilling of the soil has been
used successfully in many applications to achieve greater soil penetration. Please consult our
technical department for details.
Vhen possible, mixing can be treated by installing deep wells, French drains, etc. and allowing
for extended time.
«•
Once a layer of soil has been detoxified (as determined by lab analyses), it can be removed or
stored according to EPA guidelines and the dosage procedure can be started again on the newly
exposed toxic laden layer, if applicable.
For additional information or technical assistance, please contact:
Technical Service Department
MICROBE MASTERS, INC.
1181A Coursey Blvd.
Suite 285
Eaton Rouge, LA 70316
(504) 665-1903
105
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MASTERS, Inc.
"Biotechnology In Action"
Micro Pro Product
Efetoxifier "A"
MICH) PRO DETOXIFIER SERIES
APPiJCATICN SUGGESTIONS
Soil Contamination
Phenol, pentaphenol, cyanides, cyanates, styrene, diviny]
benezene, methycresotinate, trimethyl amine, ethylene
dichloride, dimethylatitino ethanol.
Detoxifier "B"
Polychloriiiated biphenyls, chlorinated hydrocarbons,
pentachlorophenol, chlorinated hydrocarbons.
Efetoxifier "D1
Basic general chemicals (such as "A" and "B"), oil, grease,
sulfur compounds. --~
Eetoxifier "F1
cacionics,
Eetoxifier "H"
, anionics and
ethoxylated phenols, detergents.
:tants,
, oil (refined and natural), triglycerides,
polynuclear arcmatics, napthalenes.
Used to enhance cold weather activity of natural
organisms and other Micro Pro CetoxLfier products.
Effective temperature range is 40-70* F.
106
11814 Coursey Blvd. Suite 285, Baton Rouge, LA 70816 (504) 665-1903
-------
APPENDIX C
Selected HPLC chromatograms of phase n mesocosm studies,
buried waste (2% O&G) replicates (1B2, 2B2, 3B2), day 0-42
107
-------
GDI 82 VI
45.000
mV
-5.000
7182 VIA"
45,000
mV
-5.000
14182 Vl
45.000
raV
-5.000
211B2 VI
45.000
mV
-5.000
351B2 VI
45.000
mv
-5 Oflfl
421B2 VI
45.000
mV
.000
BLANK VI
45.000
raV
-5.000
PTHALATE
45.000
raV
-5.000
AL 19 INJECT # 1 CH 2
^ A J '_^-"-_ /W*»— " *~rm» "' "-I-- - _
111 I' 1
L 3 INJECT # 1 CH 2
AL 6 INJECT # 1 CH 2
-1 ^^> •* * " "iii — r "u" "- ••"'"' ~ i i
AL 10 INJECT ft 1 CH Z
J^^1 " i i •'•" i i i •" ' '" .-i r'~ i i
0-5 10 15 20 25 30
Minutes Press RESUME
AL 10 INJECT # 1 CH 2
i L J ' ' ' ' i . .
AL 7 INJECT * 1 CH 2
i i i i i i
AL 6 INJECT * 1 CH 2
r • r - . .
ECT # 1 CH 2
0 5 10 15 20 25 30
Minutes Press RESUME
108
-------
00282 VIAL 2 INJECT tt 1 CH 2
80.000
mV
5.000
7282 VIAL~ 4 INJECT K i CH "I
75,000
raV
0.000
14282 VIAL 7 INJECT « 1 CH 2
75.000
ra9
°'000 'r „.......
212B2 VIAL 11 INJECT * 1 CH 2
75.000
rav
0.000
0 5
352B2 VIAL 11 INJECT « 1 CH 2
75.000
raO
0.000
422B2 V
75.000
nV
0.000
BLANK VIATTT
75.000
raV
0.000
10 15
Minutes
20
25 30
Press RESUME
PTHALATE VIAL 1 INJECT ft 1 CH Z
a-000
-5.000
10 IS
Minutes
20
25
30
Press RESUME
109
-------
OD3B2 VI
75.000
mv
0.000
73B2 VIA
75.000
mV
0.000
14382 VI
75.000
mV
0.000
213B2 VI
75.000
mv
0.000
353B2 VI
75.000
roV
0.000
423B2 VI
75.000
raV
0.000
BLANK VI
75.000
raV
0.000
112710 V
75.000
mV
0.000
AL 18 INJECT # i CH 2
t>> i i " wj u ir< ^ ^-' i i i
L 5 INJECT it l CH 2
11 Al A -J^V-V/^— ^^^-U^L-^V— ^-u^-^—r—
Vyvjv^
ii*"- i - ' r— niro 1 i^-Jf w I I i
AL 8 INJECT « 1 CH 2
n-k»^i • L in i 111 J i-^-^-^ — r"^"'^ i i
AL 12 INJECT ft 1 CH 2
|
0 5 10 15 20 25 30
Minutes Press RESUME
AL 12 INJECT ft 1 CH 2
AL 9 idjKil ft 1 CH i
\ _._- -n.r—
A- — ,.. ., , , 1 ^"^^
AL 6 INJECT ft 1 CH 2
IAL 2 INJECT ft 1 CH 2
i i i i i i -
0 5 10 15 20 25 30
Minutes Press RESUME
110
-------
EST-PAINE
we. ,
nn asm »v«. • BATON MOUOC. LA rauo
SAMPLE ANALYSES
for
IT CORPORATION
8124 South. Choctav Drive
Baton Rouge, Louisiana 70815
Attention: Ms. Sue Cange
January 21, 1986
dsl
oe
-------
EST-PAINE
ixc.
7979 asm AVC. • MTOM HOUOI. m rano
IT CORPORATION
Baton Rouge, Louisiana
January 21, 1986
Samples collected by IT Corporation as documented by the
enclosed chain-of-custody forms, were received at West-Paine
Laboratories, Incorporated on October 11, 1985. The samples were
analyzed according to the Environmental Protection Agency
protocol as referenced below:
A. ' Standard Methods for the Examination of Water and
Wastewater. 15th Edition, 1980:
Parameter Method
Chloride 407B
Sulfate 426C
pH 423
B) Methods for Chemical Analysis of Water and Wastes. EPA-600-
4-79-020, March, 1979:
Parameter Method
Calcium 215.1
Magnesium 242.1
Sodium ' 273.1
C) Methods of Soil Analysis. Agronomy Part 2, Chemical and
Microbiological Properties, 2nd Edition, 1982:
Parameter Method
Saturated Paste Extract 10.2
Cation Exchange Capacity 8-4
-------
p
EST-PAINE
we.
r»rt asm AVI. • IATON nouac. LA TOKO
C)
IT CORPORATION
Baton Rouge, Louisiana
January 21, 1986
Methods of Soil Analysis. Agronomy Part 2, Chemical and
Microbiological Properties, 2nd Edition, 1982: (Continued)
Parameter Method
Total Nitrogen 31-7
Total Organic Carbon 29-3.5.3
Total Phosphorous 24-2
Sodium Absorption Ratio 10-2
The results are reported on the following pages.
ard, III
dsl
-------
•p
EST-PAINE
olouz&uied INC. ,
nn GSKI AVI • §ATON MOUOC. LA ntao
IT CORPORATION
Baton Rouge, Louisiana
January 21, 1986
Sample ID: 0103 Group l. Plot 2 0-6. 10-08-85
Date Received: October 11. 19as
Parameter
Cation Exchange
Capacity (meq/lOOg)
pH (Units)
Chloride (mg/L Cl)
Sulfate (mg/L SO4)
Calcium (mg/L Ca)
Magnesium (mg/L Mg)
Sodium (mg/L Na)
Total Phosphorous
(mg/kg P)
Total Nitrogen
(mg/kg N)
Total Organic Carbon
(mg/L C)
Quality Assurance
Results Actual/Found
Date/Time
Analyst
8.2
8.1
18.5
<25
18
2.8
25
160
3.1
13,700
0.250/0.248
7.0/7.0
50/51
10.0/9.7
0.250/0.250
0.250/0.248
5.0/5.0
125/108
100/81
200/192
10-23/FD
10-23/1700/V11
10-25/0800/NB
11-07/1400/DH
10-23/FD
10-23/FD
10-23/FD
11-12/1500/HS
11-13/1700/RC
10-14/1600/NB
-------
EST -PAINE
&A INC. i
7*7* GSM A VI • IATON MOUOC. t» 70UO
IT CORPORATION
Baton Rouge, Louisiana
January 21, 1986
Sample ID: 0109 Group 2. Plot 4 0-6. 10-10-85
Date Received: October 11. 1985
Parameter
Cation Exchange
Capacity (meq/lOOg)
pH (Units)
Chloride (mg/L Cl)
Sulfate (ag/L S04)
Calcium (mg/L Ca)
Magnesium (mg/L Mg)
Sodium (mg/L Na)
Total Phosphorous
(mg/kg P)
Total Nitrogen
(mg/kg M)
Total Organic Carbon-
C)
Quality Assurance
Results Actual/Found
Date/Time
Analyst
11
7.9
5.50
<25
23
3.6
11
148
3.0
16,400
0.250/0.248
7.0/7.0
50/51
10.0/9.7
0.250/0.250
0.250/0.248
5.0/5.0
125/108
100/81
200/192
10-23/FD
10-23/1700/VH
10-25/0800/NB
11-07/1400/DH
10-23/FD
10-23/FD
10-23/FD
11-12/1500/MS
11-13/1700/RC
10-14/1600/NB
-------
f>
EST-PAINE
IMC. _
•ATOM MOUOI. LA 70HO
IT CORPORATION
Baton Rouge, Louisiana
Sanrale ID: 0112 Grouo
Data Received: October
Parameter
Cation Exchange
Capacity (meq/lOOg)
pH (Units)
Chloride (mg/L Cl)
sulfate (mg/L S04)
Calcium (mg/L Ca)
Magnesium (mg/L Kg)
Sodium (mg/L Na)
Total Phosphorous
(mg/kg P)
Total Nitrogen
(mg/Xg N)
Total Organic Carbon
(mg/L C)
January 21, 1986
2. Plot 5 0-6. 10-10-85
11. 1985
Quality Assurance
Results Actual /Found
7.8 0.250/0.248
7.7 7.0/7.0
4.50 50/51
<25 10.0/9.7
22 0.250/0.250
3.6 0.250/0.248
9.8 5.0/5.0
184 125/108
2.4 100/81
24,500 200/192
Date/Time
Analyst
10-23/FD
10-23/1700/VM
10-25/0800/NB
11-07/1400/DH
10-23/FD
10-23/FD
10-23/FD
11-12/1500/MS
11-13/1700/RC
10-14/1600/NB
*
^
-------
•p
'EST-PAINE
laubaia&yu£A we- <
rf in. •
WON MOUOL LA raao
IT CORPORATION
Baton Rouge, Louisiana
January 21, 1986
Sample ID: 0113 Control. 0-6. 10-09-85
Date Received: October 11. 1985
Parameter
Cation Exchange
Capacity (meq/lOOg)
pH (Units)
Chloride (mg/L Cl)
Sulfate (mg/L S04)
Calcium (mg/L Ca)
Magnesium (mg/L Kg)
Sodium (mg/L Na)
Total Phosphorous
(mg/kg P)
Total Nitrogen
(mg/kg H)
Total Organic Carbon
(mg/L C)
Quality Assurance
Results Actual/Found
Date/Time
Analyst
9.1
7.7
6.50
<25
18
3.1
11
153
3.6
3,660
0.250/0.248
7.0/7.0
50/51
10.0/9.7
0.250/0.250
0.250/0.248
5.0/5.0
125/108
100/81
200/192
10-23/FD
10-23/1700/VM
10-25/0800/NB
11-07/1400/DH
10-23/FD
10-23/FD
10-23/FD
11-12/1500/MS
11-13/1700/RC
10-14/1600/NB
dsl
-------
EST-PAINE
T»T» o»w AVI. • BATON flouof. LA row
IT CORPORATION
Baton Rouge, Louisiana
January 21, 1986
Sample ID: 0106 GrouD
Date Received: October
Parameter
Cation Exchange
Capacity (neq/lOOg)
pH (Units)
Chloride (mg/L Cl)
Sulfate (mg/L S04)
Calcium (mg/L Ca)
Magnesium (mg/L Mg)
Sodium (mg/L Na)
Total Phosphorous
(mg/kg P)
Total Nitrogen
(mg/kg N)
Total Organic Carbon
(ng/L C)
1. Plot 3. 0-6. 10-08-85
11. 1985
Quality Assurance
Results Actual/Found
7.9 0.250/0.248
7.7 7.0/7.0
15.0 50/51
<25 10.0/9.7
20 0.250/0.250
3.9 0.250/0.248
20 5.0/5.0
149 125/108
3.0 100/81
8,810 200/192
Date/Time
Analyst
f
10-23/5D
10-23/1700/VIt
10-25/0800/NB
11-07/1400/DH
10-23/FD
10-23/FD
10-23/FD
11-12/1500/MS
11-13/1700/RC
10-14/1600/NB
dsl
86-0321
-------
PROJECT NAME
PROJECT NO.
CHAIN OF CUSTODY RECORD
SAMPLER (SI SIGNATURE
i i
SAMPLE
IOCNTIFICATION
SAMPLIM6
LOCATION
DATE
SAMPLED
SAMPLE TYPE
10 AM OIL
VOLUME
TO BE
COLLECTED
NO OF
CONTAINERS
TIME
COLLECTION
BEGAN
TIME
COLLECTION
COMPLETED
COMMENTS
1510
1525
OlOQ?
1515
1533
01 Oj
Dili
X
uvn
RELINQUISHED BY NAME
RELINQUISHED BY
RELINQUISHED BY
AUTHORIZATION I
/HI
OIWOSAL
DATE /TIME _.
OATE/TlMf _
DATE/TINE
RECEIVED BY NAME
RECEIVED BY NAME
RCCEIVED.BY NAME
DISPOSED BY:
DATE / TIME
DATE/TIME.
DATE/TIME .
DATE/TIME.
&j£°Q
-------
'p
EST-PA1NE
tm OSMI AVL . BATON flOUaC. LA 70120
INTERNATIONAL
TECHNOLOGY
SAMPLE ANALYSES
for
IT CORPORATION
8124 South Choctaw Drive
Baton Rouge, Louisiana 70815
ATTENTION: Ms. Sue Cange
February 17, 1986
nal
86-0743
-------
EST-PAINE
ztartte& INC. <
nn asm AVI. • BATON AOUOC. LA TOUO
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Samples collected by IT Corporation as documented by the
enclosed chain-of-custody forms, were received at West-Paine
Laboratories, Incorporated on the dates indicated. The samples
were analyzed according to the Environmental Protection Agency
protocol as referenced below:
A. Standard Methods for the Examination of Water and
Wastewater. 15th Edition, 1980:
Parameter Method
Chloride 407B
Sulfate 426C
pH 423
Oil & Grease 503C
B. Methods of Soil Analysis. Agronomy Part 2, Chemical and
Microbiological Properties, 2nd Edition, 1982:
Parameter Method
Saturated Paste Extract 10.2
Cation Exchange Capacity 8-4
Total Nitrogen 31-7
Total Organic Carbon 29-3.5.3
nal
86-0743
-------
PAINE
tATON NOUOC. LA 70130
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
B. Methods of Soil Analysis. Agronomy Part 2, Chemical and
Microbiological Properties, 2nd Edition, 1982: Con't...
Parameter Method
Total Phosphorus 24-2
Sodium Absorption Ratio 10-2
The results are reported on the following pages.
Ill
Manager
nal 86-0743
J
-------
EST-PA1NE
ottftt^A we, •
r»TIQ8IH AVI. • SATON MOUQf. LA 70190
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: Control Plot «l. 11-25-85 E Q93Qhrs.
Date Received: December 23. 1985
Parameter
Oil & Grease
(mg/kg)
Total Organic
Carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
Quality Assurance
Results Actua1/Found
2,980
3,190
318
<200
9.9
16.0
41.1
2.5
8.5
Date/Time
Analyst
10.0/11.3
200/191
0.50/0.52
100/71
0.250/0.246
50.0/52.0
10.0/9.5
5.0/5.1
7.0/7.0
12-27/0900/TO
12-26/0900/RC
01-14/1730/GS
01-16/1200/RH
02-12/VM
01-20/1100/BT
01-19/2000/RC
02-05/VM
01-17/1200/RC
nal
-------
EST-PAINE
attni06 INC.
nn asm AVI • BATON nouot LA 70120
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: Group «1. Plot *2. 11-25-85 3 0939hrs.
Date Received: December 23. 1985
Parameter
Oil & Grease
(mg/kg)
Total Organic
Carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg ci)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
Quality Assurance
Results Actual/Found
8,540
9,020
250
330
9.2
10.5
42.0
1.2
8.4
10.0/11.3
200/191
0.50/0.52
100/71
0.250/0.246
50.0/52.0
10.0/9.5
5.0/5.1
7.0/7.0
Date/Time
Analyst
12-27/0900/TO
12-26/0900/RC
01-14/1730/GS
01-16/1200/RH
02-12/VM
01-20/1100/BT
01-19/2000/RC
02-05/VM
01-17/1200/RC
nal
86-0743
-------
EST-PAINE
dd urc.
mt asm AVI • SATON nouat. LA TWO
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: Group il. Plot: <3. 11-25-85 3 0946hrs.
Date Received: December 23. 1985
Parameter
Oil & Grease
(mg/kg)
Total Organic
Carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
Quality Assurance
Results Actua1/Found
7,180
11,200
259
240
9.0
13.5
42.4
1.6
8.6
10.0/11.3
200/191
0.50/0.52
100/71
0.250/0.246
50.0/52.0
10.0/9.5
5.0/5.1
7.0/7.0
Date/Time
Analyst
12-27/0900/TO
12-26/0900/RC
01-14/1730/GS
01-16/1200/RH
02-12/VM
01-20/1100/BT
01-19/2000/RC
02-05/VM
01-17/1200/RC
nal
86-074-
-------
•p
EST-PA1NE
BATON aouat LA n*»
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: Group *2. Plot »4. 11-25-85 3 0955hrs.
Date Received: December 23. 1985
Parameter
Oil & Grease
(mg/kg)
Total Organic
Carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
Quality Assurance
Results Actual/Found
5,640
6,640
268
460
9.8
13.0
50.6
1.4
8.5
10.0/11.3
200/191
0.50/0.52
100/71
0.250/0.246
50.0/52.0
10.0/9.5
5.0/5.1
7.0/7.0
1
Date/Time
Analyst
12-27/0900/TO
12-26/0900/RC
01-14/1730/GS
01-16/1200/RH
02-12/VM
01-20/1100/BT
01-19/2000/RC
02-05/VM
01-17/1200/RC
nal
86-0743
-------
'EST-PAINE
nn asm AVI • BATON ROUGE, i> rano
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: Group <2. Plot »5. 11-25-85 @ 10Q2hrs.
Date Received: December 23. 1985
Parameter
Oil & Grease
(ing/kg)
Total Organic
Carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
Quality Assurance
Results Actual/Found
11,000
10,800
279
<200
7.9
9.5
63.6
1.1
8.7
Date/Time
Analyst
10.0/11.3
200/191
0.50/0.52
100/71
0.250/0.246
12-27/0900/TO
12-26/0900/RC
01-14/1730/GS
01-16/1200/RH
02-12/VM
50.0/52.0
10.0/9.5
5.0/5.1
7.0/7.0
01-20/1100/BT
01-19/2000/RC
02-05/VM
01-17/1200/RC
nal
86-0743
-------
7*7« GSM AVI • IATON MOUQC. LA 70*30
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: 0286. 0" - 6"
Date Received: January 6. 1986
Parameter
Quality Assurance
Results Actual/Found
Date/Time
Analyst
Oil & Grease
(mg/kg) 610
Total Organic
Carbon (mg/kg C) 2,600
Total Phosphate
(mg/kg P) 248
Total Nitrogen
(mg/kg N) 320
Cation Exchange
Capacity (meq/lOOg) 10
10.0/8.8
200/185
0.50/0.52
100/71
0.250/0.246
01-13/1200/TO
01-09/0900/BT
01-14/1730/GS
01-16/1200/RH
02-12/VM
Saturated Paste
Extract:
Chloride (mg/kg Cl)
sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
20.0
52.3
1.6
8.8
50.0/52.0
10.0/9.5
5.0/5.1
7.0/7.0
01-20/1100/BT
01-19/2000/RC
02-05/VM
01-14/1145/DT
nal
86-0743
-------
EST-PAIN E
xttnieA INC.
JaJxn
nrt asm AVI. • BATON rauai. LA mao
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: 0287. 6" - 12"
Date Received: January 6. 1986
Parameter
Quality Assurance
Results Actual/Found
Date/Time
Analyst
Oil & Grease
(mg/kg) 660
Total Organic
Carbon (mg/kg C) 3,980
Total Phosphate
(mg/kg P) 257
Total Nitrogen
(mg/kg N) <200
Cation Exchange
Capacity (meq/lOOg) 7.0
10.0/8.8
200/185
0.50/0.52
100/71
0.250/0.246
01-13/1200/TO
01-09/0900/BT
01-14/1730/GS
01-16/1200/RH
02-12/VM
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
11.0
28.0
1.5
8.8
50.0/52.0
10.0/9.5
5.0/5.1
7.0/7.0
01-20/1100/BT
01-19/2000/RC
02-05/VM
01-17/1200/RC
nal
86-0743
-------
EST-PAINE
<&& IKC. i
rmosm AVI. • BATON nouot i>TOKO
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: Q288. 12" - 18"
Date Received: January 6. 1986
Parameter
Oil & Grease
(mg/kg)
Total Organic
Carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (ag/kg SO4)
Sodium Absorption
Ratio
pH (Units)
Quality Assurance
Results Actual/Found
560
1,600
300
<200
10.0/8.8
200/185
0.50/0.52
100/71
Cancelled
27.5
51.4
Cancelled
8.7
50.0/52.0
10.0/9.5
7.0/7.0
Date/Time
Analyst
01-13/1200/TO
01-09/0900/BT
01-14/1730/GS
01-16/1200/RH
01-20/1100/BT
01-19/2000/RC
01-14/1145/DT
nal
86-0743
-------
EST-PAINE
-------
EST-PAINE
£A IMC. |
TtTtOSNAVI. • tATON MOUQf. LA TOKO
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: 0290. 0" - 6"
Date Received: January 6. 1986
Parameter
Quality Assurance
Results Actua1/Found
Date/Time
Analyst
Oil & Grease
(mg/kg) 820
Total Organic
Carbon (mg/kg C) 6,530
Total Phosphate
(mg/kg P) 250
Total Nitrogen
(mg/kg N) <200
Cation Exchange
Capacity (meq/lOOg) 8.0
10.0/8.8
200/188
0.50/0.52
100/71
0.250/0.246
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
02-12/VM
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
10.0
31.1
1.2
8.5
50.0/52.0
10.0/9.5
5.0/5.1
7.0/7 .0
01-20/1100/BT
01-19/2000/RC
02-05/VM
01-17/1200/RC
nal
86-0743
-------
'p
EST-PAINE
T«T» OSm AVI • tATON HOOOI. UA 70120
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: 0291. 6" - 12"
Date Received: January 6. 1986
Parameter
Oil & Grease
(mg/kg)
Total Organic
Carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
Quality Assurance
Results Actual/Found
640
4,870
254
420
7.9
13.5
28.6
1.3
8.4
Date/Time
Analyst
10.0/8.8
200/188
0.50/0.52
100/71
0.250/0.246
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
02-12/VM
50.0/52.0
10.0/9.5
5.0/5.1
7.0/7.0
01-20/1100/BT
01-19/2000/RC
02-05/VM
01-17/1200/RC
nal
86-0743
-------
EST-PA1NE
T»T» asm AVI. • BATON nouot. i> TOKO
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: 0292. 12" - 18"
Date Received: January 6. 1986
Parameter
Quality Assurance
Results Actua1/Found
Date/Tin
Analyst
Oil & Grease
(mg/kg)
Total Organic
Carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
1,940
5,020
275
<200
Cancelled
16.5
41.6
Cancelled
8.6
10.0/8.8
200/188
0.50/0.52
100/71
50.0/52.0
10.0/9.5
7.0/7.0
01-13/1200/1
01-10/1000/F
01-14/1730/G
01-16/1200/E
01-20/1100/E
01-19/2000/F
01-17/1200/1
nal
86-0743
-------
'p
EST-PAINE
7(79 05*1 AVI. • BATON ROUGE. LA 70620
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: 0293. 18" - 24"
Date Received: January 6. 1986
Parameter
Oil & Grease
(mg/kg)
Total Organic
Carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
Quality Assurance
Results Actual/Found
Date/Time
Analyst
1,970
4,510
259
<200
Cancelled
10.0/8.8
200/188
0.50/0.52
100/71
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
20.0
36.1
Cancelled
8.4
50.0/52.0
10.0/9.5
7.0/7.0
01-20/1100/BT
01-19/2000/RC
01-17/1200/RC
nal
86-0743
-------
EST-PA1NE
ea inc.
_
T»T» asm AVI. • BATON nouoc. LA ntx
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: 0294. 0" - 6"
Date Received: January 6. 1986
Parameter
Oil & Grease
(mg/kg)
Total Organic
Carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfata (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
Quality Assurance
Results Actual/Found
1,460
4,330
241
<200
9.9
18.0
46.6
2.4
8.7
Date/Time
Analyst
10.0/8.8
200/188
0.50/0.52
100/71
0.250/0.246
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
02-12/VM
50.0/52.0
10.0/9.5
5.0/5.1
7.0/7.0
01-20/1100/BT
01-19/2000/RC
02-05/VM
01-17/1200/RC
nal
86-0743
-------
EST-PAINE
7*79 GSM AVC. • SATON ROUGE. LA 7WJO
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: 0295. 6" - 12"
Date Received: January 6. 1986
Parameter
Quality Assurance
Results Actual/Found
Date/Time
Analyst
Oil & Grease
(mg/kg) 2,550
Total Organic
Carbon (mg/kg C) 3,720
Total Phosphate
(mg/kg P) 268
Total Nitrogen
(mg/kg N) <200
Cation Exchange
Capacity (meq/lOOg) 8.8
10.0/8.8
200/188
0.50/0.52
100/71
0.250/0.246
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
02-12/VM
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO.)
Sodium Absorption
Ratio
pH (Units)
19.0
44.3
1.5
8.6
50.0/52.0
10.0/9.5
5.0/5.1
7.0/7.0
01-20/1100/BT
01-19/2000/RC
02-05/VM
01-17/1200/RC
nal
86-0743
-------
EST-PAINE
MTOM MtMC. LA 7000
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: 0296. 12" - 18"
Date Received: January 6. 1986
Parameter
Quality Assurance
Results Actual/Found
Date/Time
Analyst
Oil & Grease
(nig/kg)
Total Organic
Carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange'
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg el)
Sulfate (ag/kg SO4)
Sodium Absorption
Ratio
pH (Units)
1,480
4,450
248
<200
Cancelled
12.5
31.1
Cancelled
8.2
10.0/8.8
200/188
0.50/0.52
100/71
50.0/52.0
10.0/9.5
7.0/7.0
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
01-20/1100/BT
01-19/2000/RC
01-17/1200/RC
nal
86-0743
-------
W9 asm AVC. • IATON nouac. LA TOUO
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: 0297. 18" - 24"
Date Received: January 6. 1986
Parameter
Oil & Grease
(mg/kg)
Total Organic
Carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO.)
Sodium Absorption
Ratio
pH (Units)
Quality Assurance
Results Actual/Found
1,330
3,720
241
<200
Cancelled.
10.0/8.8
200/188
0.50/0.52
100/71
18.0
56.2
Cancelled
8.5
50.0/52.0
10.0/9.5
7.0/7.0
Date/Time
Analyst
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
01-20/1100/BT
01-19/2000/RC
01-17/1200/RC
nal
86-0743
-------
TfT* (MM AVi. • BATON MOUOC. LA 7M20
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: 0298. 0" - 6"
Date Received: January 6. 1986
Parameter
Oil & Grease
(mg/kg)
Total Organic
Carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
Quality Assurance
Results Actual/Found
5,420
9,500
268
<200
4.4
13.0
44.6
1.4
8.5
10.0/8.8
200/188
0.50/0.52
100/71
0.250/0.246
50.0/52.0
10.0/9.5
5.0/5.1
7.0/7.0
Date/Time
Analyst
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
02-12/VM
01-20/1100/BT
01-19/2000/RC
02-05/VM
01-17/1200/RC
nal
86-0743
-------
EST-PAINE
Ttrt OSMAVI. • iATON BOUOt LA TOiJO
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: 0299. 6" - 12"
Date Received: January 6. 1986
Parameter
Oil & Grease
(mg/kg)
Total Organic
Carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
Quality Assurance
Results Actual/Found
630
4,690
225
1,390
9.5
22.0
53.0
1.5
8.6
Date/Time
Analyst
10.0/8.8
200/188
0.50/0.52
100/71
0.250/0.246
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
02-12/VM
50.0/52.0
10.0/9.5
5.0/5.1
7.0/7.0
01-20/1100/BT
01-19/2000/RC
02-05/VM
01-14/1615/DT
nal
86-0743
-------
•P
EST-PAINE
nn asm AVI. • BATON nouoc LA ratio
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: 0300. 12" - 18"
Date Received: January 6. 1986
Parameter
Quality Assurance
Results Actua1/Found
Date/Time
Analyst
Oil & Grease
(mg/kg)
Total Organic
carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
410
3,720
284
<200
Cancelled
9.5
23.5
Cancelled
8.6
10.0/8.8
200/188
0.50/0.52
100/71
50.0/52.0
10.0/9.5
7.0/7.0
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
01-20/1100/BT
01-19/2000/RC
01-14/1615/DT
nal
86-0743
-------
EST-PAINE
TfTtOSm AVl • BATON NOUQC. UA 7IM20
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: 0301. 18" - 24"
Date Received: January 6. 1986
Parameter
Quality Assurance
Results Actual/Found
Date/Time
Analyst
Oil & Grease
(mg/kg)
Total Organic
Carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meg/lOOg)
Saturated Paste
Extract:
Chloride (ag/kg Cl)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
550
4,100
257
<200
Cancelled
22.0
39.3
Cancelled
8.7
10.0/8.8
200/188
0.50/0.52
100/71
50.0/52.0
10.0/9.5
7.0/7.0
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
01-20/1100/BT
01-19/2000/RC
01-14/1145/DT
nal
'86-0743
-------
•p
EST-PAINE
a&nteA INC.
_
rVTfOSMAVI. • BATON MOUGC. LA 7IM10
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: 0302. 0" - 6"
Date Received: January 6. 1986
Parameter
Oil & Grease
(mg/kg)
Total Organic
Carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
Quality Assurance
Results Actua1/Found
6,330
10,900
252
300
8.5
40.0
67.7
1.3
8.8
Date/Time
Analyst
10.0/8.8
200/188
0.50/0.52
100/71
0.250/0.246
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
02-12/VM
50.0/52.0
10.0/9.5
5.0/5.1
7.0/7.0
01-20/1100/BT
01-19/2000/RC
02-05/VM
01-14/1615/DT
nal
86-0743
-------
EST-PAINE
rrr» asm AVI. • IATON nouat, LA TOMB
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Samnle ID: 0303. 6" - 12"
Date Received: January 6. 1986
Quality Assurance
Parameter Results Actual /Found
Oil & Grease
(mg/kg) 2,910 10.0/8.8
Total Organic
Carbon (mg/kg C) 4,450 200/188
Total Phosphate
(mg/kg P) 266 0.50/0.52
Total Nitrogen
(mg/kg N) <200 . 100/71
Cation Exchange
Capacity (meq/lOOg) 10 0.250/0.246
Saturated Paste
Extract:
Chloride (mg/kg Cl) 25.0 50.0/52.0
Sulfate (mg/kg SO4) 29.9 10.0/9.5
Sodium Absorption
Ratio 1.7 5.0/5.1
pH (Units) 8.3 7.0/7.0
Date/Time
Analyst
c
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
02-12/VM
01-20/1100/BT
01-19/2000/RC
02-05/VM
01-14/1615/DT
nal
86-0743
-------
EST-PA1NE
im osm AVI • BATON aouaf. LA TOKO
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: 0304. 12" - 18"
Date Received: January 6. 1986
Parameter
Quality Assurance
Results Actual/Found
Date/Time
Analyst
Oil & Grease
(mg/kg)
Total Organic
Carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg ci)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
580
3,690
257
230
Cancelled
30.0
45.0
Cancelled
8.7
10.0/8.8
200/188
0.50/0.52
100/71
50.0/52.0
10.0/9.5
7.0/7.0
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
01-20/1100/BT
01-19/2000/RC
01-14/1615/DT
nal
86-0743
-------
EST-PAINE
rrrt asm AVC. • BATON nouoi. LA TOUO
IT CORPORATION
Baton Rouge, Louisiana
February 17, 1986
Sample ID: 0305. 18" - 24"
Date Received: January 6. 1986
Parameter
Oil & Grease
(mg/kg)
Total Organic
Carbon (mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
Quality Assurance
Results Actual/Found
Date/Time
Analyst
590
4,630
289
240
Cancelled
10.0/8.8
200/188
0.50/0.52
100/71
01-13/1200/TO
01-10/1000/RC
01-14/1730/GS
01-16/1200/RH
18.0
53.2
*
Cancelled
8.6
50.0/52.0
10.0/9.5
7.0/7.0
01-20/1100/BT
01-19/2000/RC
01-14/1615/DT
nal
86-0743
-------
SHEET NO
PROJECT NAME.
PROJECT NO_£
OQ
33
CHAIN OF CUSTODY RECORD
SAMPLERISI SIGNAT
i OT. 0\
SAMPLE
IOCNTIFICATIOM
SAMPLING
LOCATION
DATE
SAMPLED
SAMPLE TYPE
10 «m OIL
VOLUME
TO BE
COLLECTED
NO OF
CONTAINERS
TIME
COLLECTION
BEGAN
TIME
COLLECTION
COMPLETED
COMMENTS
IX
PMY
IX
p uy
<££L
£L
J2LL
V
RELINQUISHED BY NAME
RELINQUISHED BY:
RELINQUISHED BY NAME
AUTHORIZATION FOR DISPOSAL.
DATE/TIME
DATE/TIME
DATE/TIME
RECEIVED BY NAME.
RECEIVED BY: NAME .
RECEIVED BY: NAME.
DISPOSED BY:
DATE/TIME.
DATE/TIME .
DATE/TIME.
-------
CHAIN OF CUSTODY RECORD
SAMPLER (S) SIGNATUR
PROJECT NAME
PROJECT NO
TIME
COLLECTION
Z COMPLETED
RECEIVED BY NAME
RECEIVED BY: NAME
RECEIVED BY NAME
DISPOSED BY:
DATE/TIME ~
DATE/TIME
DATE/TIME
DATE/TIME
RELINQUISHED BY MAMtSX-hLfl^l'] (_
RELINQUISHED BY: NAME
RELINQUISHED BY: NAMC
AUTHORIZATION FOR DISPOSAL
-------
-------
EST-PAIN E
atoti^A INC. .
7979 QSM AVl • BATON fWUQC. LA 70620
SAMPLE ANALYSES
for
IT CORPORATION
1150 LeBlanc Road
Port Allen, Louisiana 70767
Attention: Ms. Sue Cange
June 18, 1986
dsl
86-2736
-------
nn asm AVE. • BATON MOUQC. LA TOKO
IT CORPORATION
Port Allen, Louisiana
June 18, 1986
Samples collected by IT Corporation as documented by the
enclosed chain-of-custody forms, were received at West-Paine
Laboratories, Incorporated on the dates indicated. The samples
were analyzed according to the Environmental Protection Agency
Protocol as referenced below:
A. Standard Methods for the Examination of Water and
Wastewater. 15th Edition, 1980:
Parameter Method
Chloride 407B
Sulfate 426C
pH 423
Oil & Grease 503C
B. Methods of Soil Analysis. Agronomy Part 2, Chemical and
Microbiological Properties, 2nd Edition, 1982:
Parameter Method
Saturated Paste Extract 10.2
Cation Exchange Capacity 8-4
Total Nitrogen 31-7
Total Organic Carbon 29-3.5.3
86-2736
-------
EST-PA1NE
4Z&-U641NC. mm
rtraosmAVI. • IATONnouot.LAroaao
IT CORPORATION
Port Allen, Louisiana
June IB, 1986
B. Methods of Soil Analysis. Agronomy Part 2, Chemical and
Microbiological Properties, 2nd Edition, 1982:
Parameter Method
Total Phosphorus 24-2
Sodium Absorption Ratio 10-2
The documented results are reported on the following pages.
Manager
. Blanchard, III
dsl
86-2736
-------
•p
EST-PAIN E
aJtaiatoiteA we.
7978 QSfll AVt • BATON HOUQt LA 70*20
IT CORPORATION
Port Allen, Louisiana
June 18, 1986
Sample Identification: F1197. 6" - 12"
Date Received: 04-15-86
Parameter Result
Oil & Grease (mg/kg) 758
Total Organic Carbon
(mg/kg C) 4,000
Total Phosphate
(mg/kg P) 260
Total Nitrogen
(mg/kg N) 340
Cation Exchange
Capacity (meq/lOOg) 8.8
Saturated Paste
Extract:
Chloride (mg/kg Cl) 22.5
Sulfate (mg/kg SO4) 37
Sodium Absorption
Ratio 1.4
pH (Units) 8.7
Quality Assurance
Actual/Found
10.0/9.7
200/192
0.50/0.47
5,000/3,890
0.250/0.250
50/53
10.0/9.9
0.250/0.250
7.0/7.0
Date/Time
Analyst
04-24/0830/TO
04-18/0830/KT
04-30/1500/JS
05-02/0815/KT
05-08/VM
05-12/1400/RC
05-12/1300/DT
05-08/VM
05-01/1500/DT
dsl
86-2736
-------
EST-PAINE
Ttft OSNI AVt • BATON ROUOi. LA 70UO
IT CORPORATION
Port Allen, Louisiana
June 18, 1986
Sample Identification: F1199. 18" - 24"
Date Received: 04-15-86
Parameter Result
Oil & Grease (mg/kg) 659
Total Organic Carbon
(mg/kg C) 4,500
Total Phosphate
(mg/kg P) 240
Total Nitrogen
(mg/kg N) 400
Saturated Paste
Extract:
Chloride (mg/kg Cl) 14.5
Sulfate (mg/kg SO4) 37
pH (Units) 8.5
Quality Assurance
Actual/Found
10.0/9.7
200/192
0.50/0.47
5,000/3,890
50/53
10.0/9.9
7.0/7.0
Date/Time
Analyst
04-24/0830/TO
04-18/0830/KT
04-30/1500/JS
05-02/0815/KT
05-12/1400/RC
05-12/1300/DT
04-28/1400/JS
-------
EST-PAINE
eA IKC. (
7*7* QSm AVI. • BATON flOUOC LA 70UO
IT CORPORATION
Port Allen, Louisiana
June IS, 1986
Sample Identification: F12QO. 0" - 6"
Date Received: 04-15-86
Parameter Result
Oil & Grease (mg/kg) 3,080
Total Organic Carbon
(mg/kg C) 10,000
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
260
400
6.2
21.0
35
2.0
8.9
Quality Assurance
Actual/Found
10.0/11.6
200/192
0.50/0.47
5,000/3,890
0.250/0.250
50/53
10.0/9.9
0.250/0.250
7.0/7.0
Date/Time
Analyst
04-28/1400/TO
04-18/0830/KT
04-30/1500/JS
05-02/0815/KT
05-08/VM
05-12/1400/RC
05-12/1300/DT
05-08/VM
04-24/1600/DT
dsl
86-2736
-------
EST-PAINE
6d ixc.
7979 OSRI AVC. • BATON ROUGE. LA 70120
IT CORPORATION
Port Allen, Louisiana
June 18, 1986
Sample Identification: F1201. 6" - 12"
Date Received: 04-15-86
Parameter
Oil & Grease (nig/kg)
Total Organic Carbon
(mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
Quality Assurance
Result Actual/Found
499 10.0/11.6
5,000 200/192
260 0.50/0.47
440 5,000/3,890
7.2 0.250/0.251
20.0 50/53
29 10.0/9.9
1.9 0.250/0.250
8.8 7.0/7.0
Date/Time
Analyst
04-28/1400/TO
04-18/0830/KT
04-30/1500/JS
05-02/0815/KT
06-05/YM
05-12/1400/RC
05-12/1300/DT
05-08/VM
04-24/1600/DT
-------
7t79GSfllAVI. • BATON AOUOC. LA 70820
IT CORPORATION
Port Allen, Louisiana
June 18, 1986
Sample Identification: F1202. 12" - 18"
Date Received: 04-15-86
Parameter Result
Oil & Grease (rag/kg) 910
Total Organic Carbon
(mg/kg C) 4,700
Total Phosphate
(mg/kg P) 220
Total Nitrogen
(mg/kg N) 360
Saturated Paste
Extract:
Chloride (mg/kg Cl) 21.5
Sulfate (mg/kg S04) 32
pH (Units) 8.2
Quality Assurance
Actual/Found
10.0/8.4
200/192
0.50/0.47
5,000/3,890
50/53
10.0/9.9
7.0/7.0
Date/Time
Analyst
06-09/0930/TO
04-18/0830/KT
04-30/1500/JS
05-02/0815/KT
05-12/1400/RC
05-12/1300/DT
04-28/1400/JS
dsl
86-2736
-------
ran asm AVE. • BATON nouoc. LA TOKO
IT CORPORATION
Port Allen, Louisiana
June 18, 1986
Sample Identification: F1204. 0" - 6"
Date Received: 04-15-86
Parameter Result
Oil & Grease (mg/kg) 7,290
Total Organic Carbon
(mg/kg C) 12,700
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO.)
Sodium Absorption
Ratio
pH (Units)
260
480
8.6
21.0
30
1.4
8.5
Quality Assurance
Actual/Found
10.0/11.6
200/192
0.50/0.47
5,000/3,890
0.250/0.250
50/53
10.0/9.9
0.250/0.250
7.0/7.0
Date/Time
Analyst
04-28/1400/TO
04-18/0830/KT
04-30/1500/JS
05-02/0815/KT
05-08/VM
05-12/1400/RC
05-12/1300/DT
05-08/VM
04-28/1400/JS
dsl
-------
EST-PAINE
-f
atotte&wc. ••
7979 QSW AVE. • BATON ROUOE. LA 70620
IT CORPORATION
Port Allen, Louisiana
June 18, 1986
Sample Identification: F1206. 12" - 18"
Date Received: 04-15-86
Parameter
Oil & Grease (mg/kg)
Total Organic Carbon
(mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg S04)
pH (Units)
Quality Assurance
Result Actual/Found
877 10.0/11.6
5,500 200/192
360 0.50/0.47
420 5,000/3,890
21.5 50/53
27 10.0/9.9
8.7 7.0/7.0
Date/Time
Analyst
04-28/1400/TO
04-18/0830/KT
04-30/1500/JS
05-02/0815/KT
05-12/1400/RC
05-12/1300/DT
05-01/1700/DT
OC
-------
EST-PA1NE
r<79 asm AVE. • SATON nouae. LA TOKO
IT CORPORATION
Port Allen, Louisiana
June 18, 1986
Sample Identification: F1207. 18" - 24"
Date Received: 04-15-86
Parameter
Oil & Grease (rag/kg)
Total Organic Carbon
(mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Saturated Paste
Extract: •
Chloride (mg/kg Cl)
Sulfate (mg/kg SO.)
pH (Units)
Quality Assurance
Result Actual/Found
950 10.0/8.4
4,500 200/192
340 0.50/0.47
420 5,000/3,890
15.5 50/53
32 10.0/9.9
8.4 7.0/7.0
Date/Time
Analyst
06-09/0930/TO
04-18/0830/KT
04-30/1500/JS
05-02/0815/KT
05-12/1400/RC
05-12/1300/DT
04-28/1400/JS
dsl
86-2736
-------
•p
'EST-PA1NE
IojbotCLt&u&b INC. i
nn asm AVC. • BATON ROUGE. LA rono
IT CORPORATION
Port Allen, Louisiana
June 18, 1986
Sample Identification: F1208. 0" - 6"
Date Received: 04-15-86
Parameter Result
Oil & Grease (mg/kg) 12,900
Total Organic Carbon
(mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO.)
Sodium Absorption
Ratio
pH (Units)
18,500
360
400
8.2
28.0
32
1.3
8.6
Quality Assurance
Actual/Found
10.0/11.6
200/192
0.50/0.47
5,000/3,890
0.250/0.250
50/53
10.0/9.9
0.250/0.250
7.0/7.0
Date/Time
Analyst
04-28/1400/TO
04-18/0830/KT
04-30/1500/JS
05-02/0815/KT
05-08/VM
05-12/1400/RC
05-12/1300/DT
05-08/VM
04-28/1400/JS
Otf OT-JC
-------
'p
EST-PAIN E
7979 OSMI AVt • BATON MUQE. LA 70UO
IT CORPORATION
Port Allen, Louisiana
June 18, 1986
Sample Identification: F1209. 6" - 12"
Date Received: 04-15-86
Parameter Result
Oil & Grease (mg/kg) 10,700
Total Organic Carbon
(mg/kg C) 15,200
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg S04)
Sodium Absorption
Ratio
pH (Units)
280
440
9.1
22.5
32
1.4
8.4
Quality Assurance
Actual/Found
10.0/8.4
200/192
0.50/0.47
5,000/3;-890
0.250/0.250
50/53
10.0/9.9
0.250/0.250
7.0/7.0
Date/Time
Analyst
06-09/0930/TO
04-18/0830/KT
04-30/1500/JS
05-02/0815/KT
05-08/VM
05-12/1400/RC
05-12/1300/DT
05-08/VM
04-28/1400/JS
dsl
86-2736
-------
EST-PAINE
GA we. i
nn asm AVI. • BATON NOUOE. LA mao
Parameter
Oil & Grease (mg/kg) 1,560
IT CORPORATION
Port Allen, Louisiana
June 18, 1986
Sample Identification: F1210. 12" - 18"
Date Received: 04-15-86
Quality Assurance
Actual /Found
10.0/8.4
200/192
0.50/0.47
5,000/3,890
50/53
10.0/9.9
7.0/7.0
Total Organic Carbon
(mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO4)
pH (Units)
6,600
300
280
18.0
46
8.6
Date/Time
Analyst
06-09/0930/TO
04-18/0830/KT
04-30/1500/JS
05-02/0815/KT
05-12/1400/RC
05-12/1300/DT
04-28/1400/JS
-------
•p
EST-PA1NE
^aJxn&totisA INC. i
7979 QSfll AVE. • BATON AOUGC. LA 70(20
IT CORPORATION
Port Allen, Louisiana
June 18, 1986
Sample Identification: F1211. 18" - 24"
Date Received: 04-15-86
Parameter
Oil & Grease (mg/kg)
Total Organic Carbon
(mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO.)
pH (Units)
Quality Assurance
Result Actual/Found
1,070 10.0/8.4
5,700 200/192
260 0.50/0.47
500 5,000/3,890
14.5 50/53
29 10.0/9.9
8.5 7.0/7.0
Date/Time
Analyst
06-09/0930/TO
c
04-18/0830/KT
04-30/1500/JS
05-02/0815/KT
05-12/1400/RC
05-12/1300/DT
04-28/1400/JS
I
dsl
QC
-------
•p
EST-PAINE
nn QSRI AVI. • iATON Mouot. LA rouo
IT CORPORATION
Port Allen, Louisiana
June 18, 1986
Sample Identification: F1212. 0" - 6"
Date Received: 04-15-86
Parameter Result
Oil & Grease (ing/kg) 21,900
Total Organic Carbon
(rag/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO.)
Sodium Absorption
Ratio
pH (Units)
22,900
260
380
8.2
17.0
33
1.1
8.4
Quality Assurance
Actual/Found
10.0/8.4
200/192
0.50/0.47
5,000/3,890
0.250/0.250
50/53
10.0/9.9
0.250/0.250
7.0/7.0
Date/Time
Analyst
06-09/0930/TO
04-18/0830/KT
04-30/1500/JS
05-02/0815/KT
05-08/VM
05-12/1400/RC
05-12/1300/DT
05-08/VM
04-28/1400/JS
86-2736
-------
EST-PAIN E
7979 OSNI AVI. • BATON NOUOC. LA 70(20
IT CORPORATION
Port Allen, Louisiana
June 18, 1986
Sample Identification: F1213. 6" - 12"
Date Received: 04-15-86
Parameter Result
Oil & Grease (mg/kg) 3,390
Total Organic Carbon
(mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Cation Exchange
Capacity (meq/lOOg)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO4)
Sodium Absorption
Ratio
pH (Units)
6,700
260
400
7.1
18.5
26
2.0
9.0
Quality Assurance
Actual/Found
10.0/8.4
200/192
0.50/0.47
5,000/3,890
0.250/0.250
50/53
10.0/9.9
0.250/0.250
7.0/7.0
Date/Time
Analyst
06-09/0930/TO
04-18/0830/KT
04-30/1500/JS
05-02/0815/KT
05-08/VM
05-12/1400/RC
05-12/1300/DT
05-08/VM
05-05/1400/DT
dsl
86-2736
-------
'P
EST-PAINE
nn asm AVC. • BATON «ooot LA nix
IT CORPORATION
Port Allen, Louisiana
June 18, 1986
Sample Identification: F1214. 12" - 18"
Date Received: 04-15-86
Parameter
Oil & Grease (rag/kg)
Total Organic Carbon
(mg/kg C)
Total Phosphate
(mg/kg P)
Total Nitrogen
(mg/kg N)
Saturated Paste
Extract:
Chloride (mg/kg Cl)
Sulfate (mg/kg SO.)
pH (Units)
Quality Assurance
Result Actual/Found
1,100 10.0/8.4
4,500 200/192
280 0.50/0.47
400 5,000/3,890
15.5 50/53
40 10.0/9.9
8.4 7.0/7.0
Date/Time
Analyst
06-09/0930/TO
04-18/0830/KT
04-30/1500/JS
05-02/0815/KT
05-12/1400/RC
05-12/1300/DT
04-28/1400/JS
-------
•p
'EST-PAINE
IcuboiQt&ii&A INC. i
nn asm AVI. • BATON AOUOE. LA rauo
IT CORPORATION
Port Allen, Louisiana
June 18, 1986
Sample Identification: F1215. 18** - 24"
Date Received: 04-15-86
Parameter
Result
Oil & Grease (mg/kgJ 1,180
Total Organic Carbon
(mg/kg C) 4,100
Total Phosphate
(mg/kg P) 320
Total Nitrogen
(mg/kg N) <200
Saturated Paste
Extract:
Chloride (mg/kg Cl) 19.0
Sulfate (mg/kg S04) 27
pH (Units) 8.8
Quality Assurance
Actual/Found
10.0/8.4
200/192
0.50/0.47
5,000/3,890
50/53
10.0/9.9
7.0/7.0
Date/Time
Analyst
06-09/0930/TO
04-18/0830/KT
04-30/1500/JS
05-02/0815/KT
05-12/1400/RC
05-12/1300/DT
05-18/1700/DT
86-2736
-------
SHEET NO
PROJECT NAME
PROJECT no
CHAIN OF CUSTODY RECORD
SAMPLER(S) SIGNATUR
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SAMPLE
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y/J^'foG^ 7/
-------
PROJECT NAME
PROJECT NO.
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CHAIN OF CUSTODY RECORD
SAMPLER (SI SIGNATURE
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/*
-------
'EST-PAINE
INC. _
7979 GSMI AVE. • BATON ROUGE LA 70820
SAMPLE ANALYSES
NOV 1 9
for
IT CORPORATION
8124 South Choctaw Drive
Baton Rouge, Louisiana 70815
ATTENTION: Ms. Sue Cange
November 14, 1985
PS
-------
EST-PA1NE
eA INC. i
T978 OSRI AVE • BATON ROUGE. LA 70020
IT CORPORATION
Baton Rouge, Louisiana
November 14, 1985
Samples collected by IT Corporation as documented by the
enclosed chain-of-custody forms, were received at West-Paine
Laboratories, Incorporated on October 17, 1985. The samples were
analyzed accoarding to the Environmental Protection Agency
protocol as referenced below:
A. Standard Methods for the Examination of Water
Wastewater. 15th Edition, 1980:
Parameter Method
Chemical Oxygen Demand 508A
Total Organic Carbon 505
Total Suspended Solids 209B
pH 423
The results are reported on the following pages.
and
lanager
-------
EST-PAINE
we.
7979 OSRI AVE. • BATON ROUGE. LA 70120
IT CORPORATION
Baton Rouge, Louisiana
November 14, 1985
Sample Identification: Control. Plot 1. 10-l7-85/1010hrs,
Date Received: October 17. 1985
Parameter
pH (Units)
Total Organic Carbon
(mg/L C)
Total Suspended Solids
(mg/L)
Chemical Oxygen Demand
(mg/L 02)
Quality Assurance
Results Actual/Found
8.0
2
32.7
13
7.0/7 . 0
25/23
50/49
100/109
Date/Time
Analyst
10-17/1650/NB
11-12/1200/BT
10-18/1400/KW
10-18/1050/NB
-------
EST-PAIN E
7«79 GSRI AVE • BATON ROUOE. LA 70820
IT CORPORATION
Baton Rouge, Louisiana
November 14, 1985
Sample Identification: Group 1. Plot 2. 10-17-85/1045hrs,
Date Received: October 17. 1985
Parameter
pH (Units)
Total Organic Carbon
(mg/L C)
Total Suspended Solids
(mg/L)
Chemical Oxygen Demand
(mg/L 02)
Quality Assurance
Results Actual/Found
8.0
8
157
27
7.0/7.0
25/23
50/49
100/109
Date/Time
Analyst
10-17/1650/NB
11-12/1200/BT
10-18/1400/KW
10-18/1050/NB
-------
nn asm AVE. • BATON AOUGE. LA TOUO
IT CORPORATION
Baton Rouge, Louisiana
November 14, 1985
Sample Identification: Group 1. Plot 3. 10-17-85/1100hrs,
Date Received: October 17. 1985
Parameter
pH (Units)
Total Organic Carbon
(mg/L C)
Total Suspended Solids
(mg/L)
Chemical Oxygen Demand
(mg/L 02)
Quality Assurance
Results Actual/Found
8.1
10
13.7
37
7.0/7.0
25/23
50/49
100/109
Date/Time
Analyst
10-17/1650/NB
11-12/1200/BT
10-18/1400/KW
10-18/1050/NB
-------
EST-PAINE
7979 QSRI AVE • BATON RQUQE, LA 70(20
IT CORPORATION
Baton Rouge, Louisiana
November 14, 1985
Sample Identification: Group 2. Plot 4. io-l7-85/1115hra.
Date Received: October 17. 1985
Parameter
pH (Units)
Total Organic Carbon
(mg/L C)
Total Suspended Solids
(mg/L)
Chemical Oxygen Demand
(mg/L 02)
Quality Assurance
Results Actual/Found
8.1
20
4.2
68
7.0/7.0
25/23
50/49
100/109
Date/Time
Analyst
10-17/1650/NB
11-12/1200/BT
10-18/1400/KW
10-18/1050/NB
-------
p
EST-PAINE
66 we.
7979 GSBI AVE. • BATON ROUOE. LA 70WO
IT CORPORATION
Baton Rouge, Louisiana
November 14, 1985
Sample Identification: Group 2. Plot 5. 10-17-85/113Ohrs.
Date Received: October 17. 1985
Parameter
pH (Units)
Total Organic Carbon
(mg/L C)
Total Suspended Solids
(mg/L)
Chemical Oxygen Demand
(mg/L 02)
Quality Assurance
Results Actual/Found
8.1
18
12.2
74
7.0/7.0
25/23
50/49
100/109
Date/Time
Analyst
10-17/1650/NB
11-12/1200/BT
10-18/1400/KW
10-18/1050/NB
-------
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COMMENTS
^
^
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DATT/TIMF
F B»TF/TM»
DATF/TIMT
-------
CHAIN OF CUSTODY RECORD
SHEET MO -^— Or
PROJECT NAME
M)QJF£T MO
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DATE
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r - BATt/TIMr
OATE/TI1*
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DATF/TMT
-------
p
EST-PAINE
INC.
7979 GSRI AVE • BATON ROUGE. LA 70820
rffltRNATIONAl
SAMPLE ANALYSES TECHNOLOGY
JAN 06 1986
for
IT CORPORATION
8124 South Choctaw Drive
Baton Rouge, Louisiana 70815
ATTENTION: Mr. Jeffery Cange
December 30, 1985
85-4800
nal
-------
EST-PAIN E
INC.
7979 QSPl AVE • BATON ROUGE. LA 70820
IT CORPORATION
Baton Rouge, Louisiana
December 30, 1985
Samples collected by IT Corporation as documented by the
enclosed chain-of-custody forms, were received at West-Paine
Laboratories, Incorporated on November 1, 1985. The samples were
analyzed accoarding to the Environmental Protection Agency
protocol as referenced below:
A.
Standard
Methods
for the Examination of Water
and
Wastewater. 15th Edition, 1980:
Parameter
Nitrate
Nitrite
Total Kjeldahl Nitrogen
Total Dissolved Solids
Total Organic Carbon
Specific Conductance
Method
418C
419
417A, 417B
209B
505
205
The results are reported on the following pages.
/
Burton A. Boeneke
Environmental Coordinator
nal
85-4800
-------
EST-PAINE
we.,
7979 GSRI AVE • BATON ROUGE. LA 70820
IT CORPORATION
Baton Rouge, Louisiana
December 30, 1985
Sample ID: Control Plot #1. Como. of Container's #0145 & 01A6
Date Received: November 1. 1985
Parameter
Quality Assurance
Results Actual/Found
Date/Time
Analyst
Specific Conductance
(umhos/cm)
Total Dissolved
Solids (mg/L)
680
330
720/720
11-19/1500/ML
5,000/4,945 12-02/0930/TO
nal
85-4800
-------
EST-PAINE
7979 GSBI AVE • BATON ROUGE. LA 70820
IT CORPORATION
Baton Rouge, Louisiana
December 30, 1985
Sample ID: Group 1. Plot #2. Como. of Container's #0153 & 0154
Date Received: November 1. 1985
Parameter
Quality Assurance Date/Time
Results Actual/Found Analyst
Specific Conductance
(umhos/cm)
Total Dissolved
Solids (mg/L)
700
338
720/720
11-19/1500/ML
5,000/4,945 12-02/0930/TO
nal
85-4800
-------
EST-PAINE
INC. <
7979 GSBI AVE • BATON ROUGE. LA 70420
IT CORPORATION
Baton Rouge, Louisiana
December 30, 1985
Sample ID: Group ],, Plot #3. Como. of Container's #0161.
0163 and 0164
Date Received: November 1. 1985
Parameter
Quality Assurance
Results Actual/Found
Date/Time
Analyst
Specific Conductance
(umhos/cm)
Total Dissolved
Solids (mg/L)
740
411
720/720
11-19/1500/ML
5,000/4,945 12-02/0930/TO
nal
85-4800
-------
f>
EST-PAINE
INC.
7979 GSRI AVE • BATON ROUGE. LA 70620
IT CORPORATION
Baton Rouge, Louisiana
December 30, I9B5
Sample ID: Group 2. Plot #4. Comp. of Container's #0169. 0170.
and 0171
Date Received: November 1. 1985
Parameter
Specific Conductance
(umhos/cm)
Total Dissolved
Solids (mg/L)
Quality Assurance
Results Actual/Found
Date/Time
Analyst
990
566
720/720
11-19/1500/ML
5,000/4,945 12-02/0930/TO
nal
85-4800
-------
EST-PAINE
INC.,
!><#•
7979 GSRI AVE • BATON ROUGE. LA 70820
IT CORPORATION
Baton Rouge, Louisiana
December 30, 1985
Sample ID: Group 2. Plot #5. Como. of Container's #0177. oi?fl
Date Received: November 1. 1985
Quality Assurance Date/Time
Parameter Results Actual/Found Analyst
Specific Conductance
(umhOS/cm) 765 720/720 11-19/1500/ML
Total Dissolved
Solids (mg/L) 332 5,000/4,945 12-02/0930/TO
nal 85-4800
-------
EST-PAINE
we.
7979 GSRI AVE • BATON ROUGE. LA 70820
IT CORPORATION
Baton Rouge, Louisiana
December 30, 1985
Sample ID: Group 2. Plot 15
Date Received: November 1. 1985
Parameter
Quality Assurance
Results Actual/Found
Date/Time
Analyst
Total Organic
Carbon (mg/L C)
Total Kjeldahl
Nitrogen (mg/L N)
Nitrate (mg/L N)
Nitrite (mg/L N)
10
14.56
2.6
<0.05
25/22
100/95
0.50/0.50
0.50/0.50
12-04/1600/BT
12-09/1620/RH
12-02/0900/RC
12-02/0900/RC
nal
85-4800
-------
s
ui
•fl
o
VI
»
u
is
-------
-L
PROJECT NAME
PROJECT NO
0\c\
CHAIN OF CUSTODY RECORD
SAMPLER(S) SIGNATl
REllNOUISHtl 81 NAME
RELINQUISHED BY NAME _.
RELINQUISHED BY NAME.
AUTHORISATION FOR DfSPOSAL
. DATE/TIME
. DATE/TIME
. DATE/TIME
DAT E / TIME
RECEIVED BY NAME .
PECE1.ED BY NAME
DATE/TiME
DATE/TIME.
-------
-------
CHAIN OF CUSTODY RECORD
SAMPLER(S) SIGNATURE
PROJECT NAME
PROJECT NO
RECEIVED BY
RECEIVED BY NAME
RECEIVED BY NAME
DISPOSED BY
RELINQUISHED BY NAME
RELINQUISHED BY NAME
RELINQUISHED BY NAME
AUTHORIZATION FOR DISPOSAL
-------
CHAIN OF CUSTODY RECORD
PROJECT NAME
PROJECT NO.
C
SAMPLERIS) SIGNATURE
RELINQUISHED BY
RELINQUISHED BY NAME
RELINQUISHED BY NAME
AUTHORIZATION FOR DISPOSAL.
DATE /TIME
DATE/TIME
DAT E / TIME
BECtlvED BY NAME ;
RECEIVED BY NAME .
RECEIVED BY NAME
Dl OSED BY.
DATE/TIME
DATE/TIME
DATE/TIME
DATE/TIME
-------
•p
EST-PAINE
797* OSm AVI. • BATON NOUQi. LA 70UO
SAMPLE ANALYSES
for
IT CORPORATION
8124. South Choctaw Drive
Baton Rouge, Louisiana 70815
ATTENTION: Ms. Sue Cange
January 23, 1986
I
oc
-------
EST-PAINE
JcL6otettxni0A INC.
7*71 OSNI AVI. • BATON ROUGt LA 70820
IT CORPORATION
Baton Rouge, Louisiana
January 23, 1986
Samples collected by IT Corporation as documented by the
enclosed chain-of-custody forms, were received at West-Paine
Laboratories, Incorporated on December 13, 1985. The samples
were analyzed according to the Environmental Protection Agency
protocol as referenced below:
A. Standard Methods for the Examination of Water . and
Wastewater. 15th Edition, 1980:
Parameter
Chemical Oxygen Demand
Total Organic Carbon
Total Suspended Solids
PH
Total Phosphorus
Total Dissolved Solids
Specific Conductance
Total Kjeldahl Nitrogen
Nitrate
Method
508A
505
209D
423
424C, 424F
209B
205
420A, 417A, 417D
418C
The results are reported on the following pages.
Manager
rd, III
oc noii
-------
EST-PAINE
7979GSMIAVC. • BATON HOUGE. LA 7M20
IT CORPORATION
Baton Rouge, Louisiana
January 23, 1986
Sample Identification: Control Sump <1. 0222
Date Received: December 13. 1985
Parameter
Total Organic Carbon
(mg/L C)
Chemical Oxygen Demand
(mg/L 02)
Quality Assurance Date/Time
Results Actual /Found Analyst
5
25
25/25
100/100
01-05/0600/JS-
12-19/0900/RC
-------
•p
EST-PAINE
INC. i
7979 Htm AVI. • BATON (WOO!. LA 7M20
IT CORPORATION
Baton Rouge, Louisiana
January 23, 1986
Sample Identification: Group #1. Sump #1. 0224
Date Received: December 13. 1985
Parameter
Total Organic Carbon
(ng/L C)
Chemical Oxygen Demand
(mg/L 02)
Quality Assurance / Date/Time
Results Actual/Found Analyst
9
45
25/25
100/100
01-05/0600/JS-
12-19/0900/RC
-------
EST-PAINE
eA we.,
• BATON nOUOC. LA 7M20
IT CORPORATION
Baton Rouge, Louisiana
January 23, 1986
Sample Identification: Group #1. Sump 13. 0226
Date Received: December 13. 1985
Quality Assurance Date/Time
Parameter Results Actual/Found Analyst
Total Organic Carbon
(mg/L C) 16 25/25 01-05/0600/JS-
Chemical Oxygen Demand
(mg/L 02) 54 100/100 12-19/0900/RC
-------
EST-PA1NE
A INC.
7*7* asm AVI • BATON ftouat. LA ratio
IT CORPORATION
Baton Rouge, Louisiana
January 23, 1986
Sample Identification: Group 12. Sumo 14. 0228
Date Received: December 13. 1985
Parameter
Total Organic Carbon
(mg/L C)
Quality Assurance Date/Time
Results Actual/Found Analyst
480
Chemical Oxygen Demand
(mg/L 02) 2,860
25/25
100/100
01-05/0600/JS-
12-19/0900/RC
-------
EST-PAINE
aloti6A me.
• BATON BOOOt L» 7M20
IT CORPORATION
Baton Rouge, Louisiana
January 23, 1986
Sample Identification: Grouts 12. Sumo *5. 0230
Date Received: December 13. 1985
Quality Assurance Date/Tine
Parameter Results Actual/Found Analyst
Total Organic Carbon ,
(mg/L C) 40 25/25 01-05/0600/JS-
Chemical Oxygen Demand
(mg/L 02) 145 100/100 12-19/0900/RC
-------
EST-PAINE
atonteA we. -.
r»7» OSMI AVl • IATOM MOUOf. tA 70«0
IT CORPORATION
Baton Rouge, Louisiana
January 23, 1986
Sample Identification: Control Sumo II. 0221
Date Received: December 13. 1985
Parameter
pH (Units)
Total Suspended
Solids (mg/L)
Quality Assurance Date/Time
Results Actual/Found Analyst
7.1
6.8
7.0/7.0
50/48
12-13/1630/TO
12-18/1200/TO
-------
EST-PA1NE
atoti6A INC. •
nn asm AVI. • BATON nouot. LA ratio
IT CORPORATION
Baton Rouge, Louisiana
January 23, 1986
Sample Identification: Group II. Sump #2. 0223
Date Received! December 13. 1985
Parameter
pH (Units)
Total Suspended
Solids (mg/L)
Quality Assurance Date/Time
Results Actual/Found Analyst
6.8
14.2
7.0/7.0
50/48
12-13/163 0/TO
12-18/1200/TO
-------
nn asm AVI. • SATON nouot. LA mat
IT CORPORATION
Baton Rouge, Louisiana
January 23, 1986
Sample Identification: Group <1. Sump 13. 0225
Date Received: December 13. 1985
Quality Assurance Date/Time
Parameter Results Actual/Found Analyst
pH (Units) 7.9 7.0/7.0 12-13/1630/TO
Total Suspended
Solids (mg/L) 11.8 50/48 12-18/1200/TO
-------
EST-PA1NE
6A INC.
7979 S»m AVi • BATON ROUQl LA 7M20
IT CORPORATION
Baton Rouge, Louisiana
January 23, 1986
Sample Identification: Group *2. Sumo *4. 0227
Date Received: December 13. 1985
Quality Assurance Date/Time
Parameter Results Actual/Found Analyst
pH (Units) 7.9 7.0/7.0 12-13/1630/TO
Total Suspended
Solids (mg/L) 15.6 50/48 12-18/1200/TO
I
-------
EST-PAINE
A 1KC. i
797» QSMI AVI. • SATON HOUdf. LA 7QOO
IT CORPORATION
Baton Rouge, Louisiana
January 23, 1986
Sample Identification: Group »2. Sumo 15. 0229
Date Received: December 13. 1985
Parameter
pH (Units)
Quality Assurance
Results Actual/Found
Date/Tine
Analyst
7.9
7.0/7.0 12-13/1630/TO
-------
EST-PAINE
A INC. <
nn QSM Ave. • BATON aouat, LA rota
1
IT CORPORATION
Baton Rouge, Louisiana
January 23, 1986
Sample Identification: Group *1. Plot 12. 0233
Date Received: December 13. 1985
Quality Assurance Date/Time
Parameter Results Actual/Found Analyst
pH (Units) 7.8 7.0/7.0 12-13/1630/TO
Total Organic
Carbon (mg/L C) 20 25/22 01-07/0800/RC
Total Phosphate
(mg/L P) 0.06 1.0/1.0 01-02/1000/RG
-------
EST-PAINE
atatieA INC. •
• BATON MOUQC. LA 70820
IT CORPORATION
Baton Rouge, Louisiana
January 23, 1986
Sample Identification: Group II. Plot »3. 0235
Date Received: December 13. 1985
Parameter
Quality Assurance
Results Actual/Found
Date/Time
Analyst
pH (Units)
Total Dissolved
Solids (mg/L)
Specific Conductivity
(umhos/cm)
7.8
569
840
7.0/7.0 12-13/1630/TO
5,000/5,088 12-18/1000/TO
720/720 01-13/1500/MS
-------
EST-PAINE
IMC. i
nn asm AVE. • §ATON aouat LA TOHO
IT CORPORATION
Baton Rouge, Louisiana
January 23, 1986
Sample Identification: Group »2. Plot *5. 0239
Date Received: December 13. 1985
Parameter
pH (Units)
Total Organic
Carbon (mg/L C)
Total Phosphate
(ng/L P)
Total Dissolved
Solids (ng/L)
Specific Conductivity
(umhos/cm)
Total Kjeldahl
Nitrogen (mg/L N)
Nitrate (mg/L N)
Nitrite (mg/L N)
Quality Assurance
Results Actual/Found
7.7
7
0.06
628
850
2.9
<0.05
<0.05
7 . 0/7 . 0
25/22
1.0/1.0
Date/Time
Analyst
12-13/1630/TO
01-07/08 00/RC
01-02/1000/RG
5,000/5,088 12-18/1000/TO
720/720 01-13/1500/MS
100/99
0.50/0.50
0.50/0.50
12-26/1150/RG
12-31/1000/RG
12-31/1000/RG
-------
'P
EST-PAINE
attn±&> we. —
nn asm *vt • BATON MOUQI. t* TOKO
IT CORPORATION
Baton Rouge, Louisiana
January 23, 1986
Sample Identification: Group 12. Plot *4. 0247
Date Received: December 13, 1985
Parameter
pH (Units)
Total Dissolved
Solids (mg/L)
Specific Conductivity
(umhos/cm)
Quality Assurance
Results Actual/Found
7.9
7.0/7.0
Date/Time
Analyst
12-13/1630/TO
705
5,000/5,088 12-18/1000/TO
Insufficient Sample
-------
EST-PAINE
'jJA tur ,
7*79 QSMI AVf. • BATON MOUOf. LA 7IN30
IT CORPORATION
Baton Rouge, Louisiana
January 23, 1986
Sample Identification: Group 12. Plot *5. 0249
Date Received: December 13. 1985
Parameter
pH (Units)
Total Organic
Carbon (mg/L C)
Total Dissolved
Solids (mg/L)
Specific C iductivity
(umhos/cm)
Quality Assurance Date/Time
Results Actual/Found Analyst
7.8
8
670
820
7.0/7.0
25/22
12-13/1630/TO
01-07/0800/RC
5,000/5,088 12-18/1000/TO
720/720
01-13/1500/MS
-------
•p
EST-PAINE
7*79 03*1 AVI • RATON HOUOi LA TOKO
IT CORPORATION
Baton Rouge, Louisiana
January 22, 1986
Sample Identification: Group tl. Plot *3. 0245
Date Received: December 13. 1985
Parameter
Total Organic
Carbon (mg/L C)
Total Phosphate
(ag/L P)
Total Kjeldahl
Nitrogen (mg/L N)
Nitrate (mg/L N)
Nitrite (mg/L N)
Quality Assurance
Results Actual/Found
Date/Time
Analyst
17
<0.05
7.1
<0.05
<0.05
25/22
1.0/1.0
100/99
0.50/0.50
0.50/0.50
01-07/0800/RC-
01-02/1000/RG
12-26/1150/RH
12-31/1000/RG
12-31/1000/RG
-------
EST-PAINE
INC.
T»T» asm AVI • BATON MOUOE. LA TOKO
IT CORPORATION
Baton Rouge, Louisiana
January 23, 1986
Sample Identification: Group 12. Plot »4. 0237
Date Received: December 13. 1985
Quality Assurance Date/Time
Parameter Results Actual/Found Analyst
Total Organic
Carbon (ng/L C) 21 25/22 01-07/0800/RC.
Total Phosphate
(mg/L P) <0.05 1.0/1.0 01-02/1000/RG
I
•* s* /%«*«•«
-------
EST-PAINE
we.,
rtn asm AVE • «ATON nouat. L» rouo
IT CORPORATION
Baton Rouge, Louisiana
January 23, 1986
Sample Identification: Group *l. Plot *2. 0243
Date Received: December 13. 1985
Quality Assurance Date/Time
Parameter Results Actual/Found Analyst
Total Dissolved
Solids (mg/L)C) 21721 5,000/5,088 12-18/1000/TO
«
Specific Conductivity
(umhos/cm) Insufficient Sample
nal 86-0311
-------
INTERNATIONAL
TECHNOLOGY
CORPORATION
PROJECT NAME/NUMBER
CHAIN-OF-CUSTODY RECORD
LAB DESTINATION _1
R/A Control No.
C/C Control No. Q006461
/
•^jr
SAMPLE TEAM MEMBERS TV \nt Wx\n ^i^L JT-H . !
CARRIER/WAYBILL NO.
Sample
Number
Sample
Location and Description
Dale and Time
Collected
Sample
Type
Container
Type
Conditlon on Receipt
(Name and Date)
Disposal
Ccnrjrrl-Sornp .1
^ Tor
J.
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1^3.
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Special Instructions:
C "
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Possible Sample Hazards:
SIGNATURES: (Name. Company, Date
1. Relinquished
Received By
2. Relinquished By:
Received By:
3. Relinquished By:
Received by:
4. Relinquished By:
Received By:
WHITE - To accompany tamplas
YELLOW -FI«W copy
-------
R/A Control No.
Luc
PROJECT N
SAMPLE TE
Sample
Number
O33I
G^s:^
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0337
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SIGNATURE
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OlRPORATIoJl CHAIN-OF-CUa fOI
lAMF/NI IMRPR C'6-t^ 1 V^k>£R "^ b S~Of 1 i
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(Vvrtvc* /^tc-T 1 ^J^V, Con^'.
GU«SS T«4J/r>
^_i;n'vvt;\ , V^^^X, tSioc^-> r*B^*>
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Date and Time
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^(\3/"J'i 2'2-D
iMisy?*- Z'p^
IZJOfT^ p jj^j
it.]i?/s5 ^2*^5
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WHITE - To accompany tampta
YELLOW -FMd copy
-------
"tKNATIUNAL
.-CHNOLOGY
CORPORATION
PROJECT NAME/NUMBER
SAMPLE TEAM MEMBERS
R/A Control No.
\KNL-Ft7-. fs5"~ 5Of
CHAIN-OF-CUSTODY RECORD
C/C Control No. Q00650
LAB DESTINATION it 'K8* 11 riL\ fl g ^L(3 b *3
O V^
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CARRIER/WAYBILL NO.
Sampla
Number
Sample
Location and Description
Date and Time
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Sample
Type
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Type
Condition on Receipt
(Name and Date)
Dlspoul
Record No.
*«•'.
O
c.fTl|c.oi>'J, ^cnsi.
^ Tor
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Special Instructions:
Possible Sample Hazards:
SIGNATURES: (Name. Company, Date and Time)
1. Relinquished I
Received By:_^
3. Relinquished By:
Received by:
2. Relinquished By:
Received By:
4. Relinquished By:
Received By:
WHITE - To »ccomp«ny Mmptot
YELLOW -FMd copy
-------
EST-PAINE
INTERNATIONAL
TECHNOLOGY
JlAR201QRn
7979~GSRI AVE. • BATON ROUQ8. LA 70820
SAMPLE ANALYSES
for
IT CORPORATION
8124 South Choctaw Drive
Baton Rouge, Louisiana 70815
Attention: Ms. Sue Cange
March 14, 1986
86-1200
-------
EST-PAINE
7979 QSRI AVE. • BATON SOlXJt LA 70830
IT CORPORATION
Baton Rouge, Louisiana
March 14, 1986
Samples as designated below, and on the enclosed chain-of-
custody documentation, were received at West-Paine Laboratories,
Incorporated on February 6, 1986. The samples were analyzed for
the requested parameters according to the Environmental
Protection Agency protocol:
A. Standard Methods for the Examination of Water and
Wastewater. 15th Edition, 1980:
Parameter
pH
Specific Conductance
Total Dissolved Solids
Total Suspended Solids
Total Kjeldahl Nitrogen
Chemical Oxygen Demand
Total Organic Carbon
Nitrate/Nitrite
Total Phosphate
Method
423
205
209B
209D
420A, 417A, 417D
508
505
418C
424C, 424F
The results are reported on the following pages.
nchard, III
Manager
dsl
86-1200
-------
EST-PA1NE
te* INC.
7979 QSRI AVt • BATON TOUCH. LA 70820
IT CORPORATION
Baton Rouge, Louisiana
March 14, 1986
Sample Identification: SW. Control Plot II. 1259 & 1260
Date Received: 02-06-86
Parameter
pH (Units)
Total Suspended Solids
(mg/L)
Total Organic Carbon
(mg/L C)
Chemical Oxygen Demand
(mg/L 02)
Quality Assurance Date/Time
Results Actual/Found Analyst
7.8
37
14
7.0/7.0
50.0/48.8
25/23
100/94
02-06/1700/KT
02-06/2130/TO
02-16/1400/RC
02-10/0830/KT
oc 1
-------
•p
EST-PA1NE
7979 GSM AVC • BATON HOUOE. LA 70820
IT CORPORATION
Baton Rouge, Louisiana
March 14, 1986
Sample Identification: SW. Plot 2-5. 1261 & 1262
Date Received: 02-06-86
Parameter
pH (Units)
Total Suspended Solids
(mg/L)
Total Organic Carbon
(mg/L C)
Chemical Oxygen Demand
(mg/L 02)
Quality Assurance Date/Time
Results Actual/Found Analyst
7.9
132
29
110
7.0/7.0
50.0/48.8
25/23
100/101
02-06/1700/KT
02-06/2130/TO
02-16/1400/RC
02-12/0930/KT
dsl
-------
7979 GSRI AVE. • BATON ROUQE. LA 70820
IT CORPORATION
Baton Rouge, Louisiana
March 14, 1986
Sample Identification: Control Plot #1 1263 & 1275
Date Received: 02-06-86
Parameter
pH (Units)
Specific Conductance
(umhos/cm)
Total Dissolved Solids
(mg/L)
Total Organic Carbon
(mg/L C)
Quality Assurance Date/Time
Results Actual/Found Analyst
8.0
680
937
26
7.0/7.0
720/720
25/23
02-06/1700/KT
02-25/1630/GS
5,000/4,918 02-06/2300/TO
02-16/1400/RC
rlcl
-------
EST-PAINE
7979 GSR! AVE. • BATON ROUGE. LA 70820
IT CORPORATION
Baton Rouge, Louisiana
March 14, 1986
Sample Identification: Group 1. Plot #2 1264 & 1274
Date Received: 02-06-86
Parameter Results
pH (Units) 7.7
Specific Conductance
(umhos/cm) 1,000
Total Dissolved Solids
(mg/L) 1,030
Total Kjeldahl Nitrogen
(mg/L N) 3.5
Nitrate (mg/L N) 0.05
Nitrite (mg/L N) <0.05
Total Organic Carbon
(mg/L C) 20
Total Phosphate
(mg/L P) <0.1
Quality Assurance Date/Time
Actual/Found Analyst
7.0/7.0
720/720
100/102
0.50/0.50
0.50/0.50
25/23
1.00/1.04
02-06/1700/KT
02-25/16-30/GS
5,000/4,918 02-06/2300/TO
02-14/1200/RH
02-18/0800/DH
02-18/0800/DH
02-16/1400/RC
02-13/1400/RH
dsl
86-1200
-------
EST-PAINE
ICfjbo
7979 asm AVE. • BATON nouac. LA TOUO
IT CORPORATION
Baton Rouge, Louisiana
March 14, 1986
Sample Identification: Group 1. Plot #3 1265 & 1272
Date Received: 02-06-86
Parameter Results
pH (Units) 7.3
Specific Conductance
(umhos/cm) 1,000
Total Dissolved Solids
(mg/L) 745
Total Kjeldahl Nitrogen
(mg/L N) 2.03
Nitrate (mg/L N) 0.35
Nitrite (mg/L N) <0.05
Total Organic Carbon
(mg/L C) 10
Total Phosphate
(mg/L P) <0.1
Quality Assurance Date/Time
Actual/Found Analyst
7.0/7.0
720/720
5,000/4,918
100/102
0.50/0.50
0.50/0.50
25/23
1.00/1.04
02-06/1700/KT
02-25/1630/GS
02-06/2300/TO
02-14/1200/RH
02-18/0800/DH
02-18/0800/DH
02-16/1400/RC
02-13/1400/RH
dsl
86-1200
-------
EST-PAINE
7979 GSRI AVt • BATON POUQt LA 70820
IT CORPORATION
Baton Rouge, Louisiana
March 14, 1986
Sample Identification: Group 2. Plot #4 1266. 1270. & 1271
Date Received: 02-06-86
Parameter Results
pH (Units) 7.0
Specific Conductance
(umhos/cm) 1,100
Total Dissolved Solids
(mg/L) 792
Total Kjeldahl Nitrogen
(mg/L N) 2.1
Nitrate (mg/L N) 2.9
Nitrite (mg/L N) <0.05
Total Organic Carbon
(mg/L C) 5
Total Phosphate
(mg/L P) <0.1
Quality Assurance Date/Time
Actual/Found Analyst
7.0/7.0
1,400/1,400
5,000/4,918
100/102
0.50/0.50
0.50/0.50
25/23
1.00/1.04
02-06/1700/KT
02-25/1630/GS
02-06/2300/TO
02-14/1200/RH
02-18/0800/DH
02-18/0800/DH
02-16/1400/RC
02-13/1400/RH
dsl
86-1200
-------
EST-PA1NE
IaJtotototi
7979 GSfll AV6. • BATON ROUGE. l> 70MO
IT CORPORATION
Baton Rouge, Louisiana
March 14, 1986
Sample Identification: Group 2. Plot #5 1267. 1268, & 1269
Date Received: 02-06-86
Parameter Results
pH (Units) 7.2
Specific Conductance
(umhos/cm) 1,000
Total Dissolved Solids
(mg/L) 694
Total Kjeldahl Nitrogen
(mg/L N) 1.26
Nitrate (mg/L N) 1.2
Nitrite (mg/L N) <0.05
Total Organic Carbon
(mg/L C) 10
Total Phosphate
(mg/L P) <0.1
Quality Assurance Date/Time
Actual/Found
7.0/7.0
02-06/1700/KT
1,400/1,400 02-25/1630/GS
5,000/4,918 02-06/2300/TO
100/102
0.50/0.50
0.50/0.50
25/23
1.00/1.04
02-14/1200/RH
02-18/0800/DH
02-18/0800/DH
02-16/1400/RC
02-13/1400/RH
rkl
-------
\-\
CHAIN OF CUSTODY RECORD
PROJECT NAME
PROJECT MO
SAMPLER(S) SIGNATUR
RELINQUISHED BY: NAME
RELINQUISHED BY NAME
JK'THORIZATION FOR DISPOSAL.
DATE/TIME
DATE/TIME
DATE /TIME
BY
RECEIVED BY: NAME .
RECEIVED BY: NAME .
DISPOSED BY:
DATE /TIME ±jjt f **> /VJ"
DATE/TIME
DATE/TIME .
DATE /TIME.
- 6^lc\55 6 1
J>v>
-------
SHEET NO
PROJECT NAME
PROJECT NO.
CHAIN OF CUSTODY RECORD
SAMPLER(S) SIGNATUR
SAMPLE
IDENTIFICATION
SAMPLING
LOCATION
DATE
SAMPLED
SAMPLE TYPE
ID AIM OIL
VOLUME
TO BE
COLLECTED
NO. OF
CONTAINERS
TIME
COLLECTION
BEGAN
TIME
COLLECTION
COMPLETED
COMMENTS
.12.7:5
L
Ar
!. }..
RELINQUISHED BY
RELINQUISHED BY- NAME
RELINQUISHED BY NAME
AUTHORIZATION FOR DISPOSAL.
t/ATE /TIME
DATE/TIME
DATE/TIME
DATE/TIME
//&/$&
RECEIVED BY MAME .
RECEIVED BY: NAME .
RECEIVED BY: NAME .
DISPOSED BY:
DATE/Tit
DATF/TIMF
-------
.KiTCRNATtONAL
OLD IN6ER FIELD PLOT STUDIES
MICROTOX SOIL CORE WSF RESULTS
1 £V^r11'**
^njrArJj
"k re.
. n0 iQflfi — -T- —
DEf TN1 U °
SAMPLE
EC50
\\fCfji- \ I /
iv- '-'-*•• 1 j c
LL5
7 -p\ fJU
I C**
SAMPLE
'#
"^r
EC50
LL5
SAMPLE
EC50
LL5
0-6"
6-12"
12-18"
18-24"
0-6"
6-12"
12-18"
18-24"
0-6"
6-12"
12-18"
18-24"
0-6"
6-12"
12-18"
18-24"
0-6"
6-12"
12-18"
18-24"
0306
0307
0308
0309
0310
0311
0312
0313
0314
0315
0316
0317
0318
0319
0320
0321
0322
0323
0324
0325
OTE
NTE
NTE
NTE
NTE
NTE
NTE
S
OTE
S
NTE
NTE
OTE
S
S
NTE
NTE
S
S
NT
22 0266
-3
17
15
— -RRflllP
-1
-3
3
-7
.GROUP
28
-19
3
8
rpniip
28
-13
-8
5
....GROUP
10
-19
-13
NT
0267
0268
0269
1, PLOT
0270
0271
0272
0273
1, PLOT
0274
0275
0276
0277
2, PLOT
0278
0279
0280
0281
2, PLOT
0282
0283
0284
0285
NTE
NTE
NTE
OTE
7 ....
99
OTE
NTE
NTE
1 ....
97
OTE
NTE
NTE
A ....
OTE
NTE
NTE
NTE
5....
70
OTE
NTE
NTE
19
18
15
24
33
35
3
17
39
22
19
4
27
14
10
10
50
28
18
7
3121
3122
3123
3124
3125
3126
3127
3128
3129
3130
3131
3132
3133
3134
3135
3136
3137
3138
3139
3140
OTE
OTE
OTE
NTE
75
OTE
76
NTE
62
OTE
83
NTE
OTE
OTE
OTE
NTE
90
OTE
OTE
NTE
30
21
22
15
47
33
36
11
55
35
46
7
37
31
33
3
47
37
28
8
EC50 Vol % of the distilled, de ionized water (DW) extract effecting a 50% decrease
1n b1olum1nescence during a 5 minute test period. The DW extract 1s obtainec
by shaking 400 ml DW + 100 g sample for 24 hours.
LL5 The 5 minute mean X light loss 1n the combined full strength and 50% serial
dilutions of the DW extract.
NTE No observed Toxic Effect [reported when the LL5 1s less than 20X; 1f the LL5
1s less than -5X, stimulation (S) of blolumlnescence 1s reported].
OTE Observed Toxic Effect [reported when a definite loss of light 1s observed
(LL5>20X); however, the level of decrease 1n blolumlnescence 1s such that
a quantitative EC50 cannot be established].
NT Sample not tested.
-------
SHEET NO
PROJECT NAME
CHAIN OF CUSTODY RECORD
SAMPLER (S) SIGNATURE
RELINQUISHED BY
RELINQUISHED BY: NAME
RELINQUISHED BY NAME
AUTHORIZATION FOR DISPOSAL.
. DATE/TIME
DATE/TIME
. DATE/TIME
DATE/TIME
XCIVED BY NAME.
RECEIVED BY: NAME .
RECEIVED BY: NAME.
DISPOSED BY=
DATE/TIME
DATE/TIME -
DATE/TIME .
DATE/TIME.
-------
SHEET NO
PROJECT NAME
PROJECT NGk
CHAIN OF CUSTODY RECORD
SAMPLER(S) SIGNATURE
RELINQUISHED BY:
RELINQUISHED BY:
RELINQUISHED BY
AUTHORIZATION FOR DISPOSAL .
NAME
.DATE/TIME <
. DATE/TIME
. DATE/TIME
. DATE/TIME .
:CEIVED BY NAME .
RECEIVED BY: NAME .
RECEIVED BY: NAME
DISPOSED BY:
OATf/TIMF
DATE/TIME.
DATE/TIME.
-------
6TAT1CM
CO ATE
3030
3037
J033
JA34
3039
3037
303H
30«A
jA4l
3042
3043
3044
3049
J046
30*7
3048
J049
3ASO
3051
3053
30S4
30*9
305*
3057
30S9
30bA
3Ao2
30o3
30e4
3Ao9
30e*
30oB
30o9
JA70
3071
4072
3073
3074
3A75
3A76
3077
3074
30*0
30«1
30»3
3A»4
30*5
30»6
30*3
3094
3099
JlgO
3101
31u7
31w3
lei
11»7
lib*
115*
1909
1910
1914
191S
1917
1918
19-^0
1922
19*3
1929
192*
19*7
1928
193ft
1931
194?
1934
1937
193"
1939
1940
1941
1942
1943
1944
1949
19*7
19,«
3037
J040
3041
3042
30*7 JA44
1 H.uT 7 0-«" 0101
3 u-o- olo4
4 0-*« 010?
it 9 A-6'
2 0-*"
3 0-6"
PLOT 4 ft.*- »1*10
9 0-*"
0-^" |0^0b
G»OI'f I ?1;oT 2 0-
§020*
0?«6
02e*
0770
0771
0772
0273
0274_
u279
0776
0277
027«
INTERNATIONAL
TECHNOLOGY
JUM06198G
02*7
0^12
C313
0'14
0*16
0317
0319
03*o
03k?
03.4
i mor
CPOUP i, PLOT * §1302
CPuUP l.ftCT 3 tl3o1
PbOT b
••f.CiT I «U73
CONTHOt, «•! 0T 1 41374
CPullr 1.
1,
H32*
tl*27
PI.CT 3
(,»uUP \, PbCT 3
1. PLCt * tl331
i., PUCT 'i «l3i2
4
«,»C-"t» <
» §1337
09/2U/»9
10/0&/b5
in/03/k5
10/10/89
10/10/09
09/20/**
09/70/b9
09/20/«9
11/29/U9
11/7S/H5
11/79/H9
11/25/49
11/25/89
01/2'4/bA
01/22/86
01/22/H6
OI/22/K6
01/22/86
01/22/S6
01/22/66"
01/22/86
01/22/86
01/22/86
01/22/86
01/22/86
01/22/86
01/22/86
01/22/86
01/22/86
01/22/B6
01/22/86
01/22/86
01/22/86
01/22/06
01/22/K6
01/22/06
01/22/86
01/22/86
01/2^/bfi
01/22/M6
01/22/H6
01/22/86
01/22/86
01/22/86
01/2^/66
01/22/86
01/22/U6
01/22/8*
01/72/86
01/22/8*
01/2-^/86
01/22/M6
U3/10/86
03/10/MA
03/10/86
03/10/86
03/10/M6
04/15/»6
04/lb/H6
04/15/H6
04/15/M6
04/tb/h6
04/15/B6
04/lb/d«
04/15/4i6
04/15/B6
U4/13/B6
04/ls/e6
04/15/«6
04/l«/«6
04/la/k*
04/lb/»6
04/ls/b*
-------
PROJECT: mo INGERS SAMPLES COLLECTED 4/is/w
W.13
PRECISION REPORT
OUFUCATE ANALYSIS DATA
ABURPT.LSTJ671
CONCENTRATION IN N6/K6 DRY UT.
SAHP
NO
TIME
DATE
ELMT
NA
K
CA
N6
FE
m
CO
NO
AL
AS
SE
CD
BE
01
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
3036 * ANALYSIS DATA
NO * LESS THAN LOD
ZRD * PERCENT RELATIVE DIFFERENCE
EPA/RSKERL/ADA.OK
-------
PRECISION REPORT
DUPLICATE ANALYSIS DATA
ADURPT.LST?666
PROJECT: OLD INGERS SITE SANPLES COLLECTED 3/10/86
CONCENTRATION IN N6/K6 DRY «T.
SAMP
NO
TINE
DATE
ELHT
NA
K
CA
H6
FE
HN
CO
NO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
AG
TL
PB
LI
SR
V
BA
B
TI
2483M)
18113
22HAY86
AVERAGE
111.
1240.
4S10.
2370.
16900.
562.
7.35
NO
15300.
ND
ND
ND
0.907
7.2?
18.9
11.4
34,7
ND
NO
13.5
8.61
23.4
33.7
218.
5.53
325.
ZRD
1.76
3.38
2.06
0.94
0.17
1.41
3.03
2.43
7.09
2.28
1.18
2.72
1.38
9.36
3.4
1.54
3.35
0.56
1.81
0.84
2487(A)
17:28
22HAY86
AVERAGE
150.
1650.
5290.
2700.
15800.
467.
6.9
5.43
17700.
ND
ND
ND
0.93
9.8S
24.5
14.
43.3
ND
ND
17.9
11.6
28.9
36.5
250.
6.48
368.
ZRD
7.4
1,39
•3.84
0.42
-3.76
-30.74
-29.15
-3.16
0.22
-0.97
-1,46
-3,8
-8,61
4.19
-19.21
2.06
4.84
-5.09
-5.9
-21.62
0.42
AVERA6E ZRD
AVERAGE ZRD
AVERAGE ZRD
* NEASURENENT DATA
(A) * ANALYSIS DATA
ND * LESS THAN LOO
ZRD = PERCENT RELATIVE DIFFERENCE
EPA/RSKERL/ADAiOK
-------
PRECISION REPORT
DUPLICATE ANALYSIS DATA
ADURPT.LSTJ663
PROJECT: OLD INGERS SITE SAMPLES COLLECTED 1/22/86
P9.9
CONCENTRATION IN N6/K6 DRY VT.
SAflP
NO
TINE
DATE
ELHT
NA
K
CA
H6
FE
HN
CO
HO
AL
AS
SE
CD
BE
CU
SB
CR
NI
ZN
A6
TL
PB
HG
LI
TE
SR
6E
V
DA
B
TI
1929
-------
PRECISION REPORT
DUPLICATE ANALYSIS DATA
ADURPT.LSTJ661
PROJECT: OLD INGERS SHE SAMPLES
COLLECTED 1/22/86
P9.1
CONCENTRATION IN H6/K6
SANP
NO
TIKE
DATE
ELNT
MA
K
CA
MS
FE
m
CO
HO
AL
AS
SE
CD
BE
CU
SB
CR
NI
ZN
AG
TL
PB
HG
LI
TE
SR
6E
V
BA
B
TI
1909(N)
10M7
7NAY86
AVERAGE
149.
1550.
4950.
2690.
14800.
257.
4.96
KB
17100.
ND
14.2
KD
0.85
7,74
ND
22.2
13,4
37.2
NO
ND
14,1
ND
8.77
NO
24.6
ND
31.8
164.
6.65
338.
1909CA)
10
:o3
7NAY86
ZRD
6.28
6.17
0.76
3.54
3.38
2.43
1.22
2.91
5.91
3.74
6.38
1.81
1.04
1,47
18.94
9.97
3.52
4.59
3,2
8.14
0.24
AVERAGE
ISO.
1550.
4820.
2740.
15300.
288.
5.33
ND
17200.
ND
13.4
ND
0.878
7.92
ND
22,1
13.3
37.
NO
ND
13.5
ND
9.11
ND
25.
MB
32.4
174.
6.44
322.
ZRD
4.32
5.78
6.22
-0.34
-3.64
-19.47
-12.75
1.57
6.31
-2.79
1.56
2.69
2.47
2.54
-11.18
2.11
0.86
0.68
-6.64
14.71
10.42
1917(A)
10:24
7HAY86
AVERAGE
161.
1390.
4420.
2590.
14500.
246.
4.84
ND
16700.
KD
14.3
ND
0.826
6.77
ND
21.8
12.3
35.5
ND
ND
14.9
ND
7.97
ND
23.9
ND
30.4
160.
7.34
316.
ZRD
-6.2
-11.67
3.76
-3.3
-1.67
11.55
2.01
-4.06
-13.7
-11.75
-7.14
-2.06
-2.51
-1.27
-1,54
-6.71
-1.95
-2.43
-4.07
1.84
-6.21
1928(A)
10
M6
7HAY86
AVERAGE
144.
1270.
6450.
2300.
13000.
235.
4.62
NO
15600.
MB
12.7
ND
0.738
6.08
ND
20.9
11.6
33.9
ND
ND
16.1
NO
7,2
NO
34.5
NO
28.2
151.
4.18
292.
ZRD
-11.76
13.51
-57.6
2.06
2.32
3.91
5.64
3.59
7.81
8.86
1,09
6.53
4.65
0.7
5.96
1.4
-61.85
5.62
2.32
-40.93
21.7
AVERAGE ZRD
(H) > NEASURENENT DATA
(A) * ANALYSIS DATA
NO * LESS THAN L09
ZRD * PERCENT RELATIVE DIFFERENCE
EPA/RSKERL/ADA>OK
-------
PROJECT: on INGERS SITE T.A.
P1B4.8
CONCEKTRAnON IM N6/K6 VET UT.
PRECISION REPORT
DUPLICATE ANALYSIS MTA
ADURPT.LSTJ651
SAMP
NO
626 (H)
1155
2006 (A)
1155
336 (A) 4366(A)
11S9DUP GRIND 1159 DUP 016
OFDUP6RIMB
TINE
DATE
ELNT
NA
K
CA
M6
FE
W
CO
HO
AL
AS
SE
CD
BE
CU
CR
HI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
n:o?
300EC8S
AVERAGE
73.6
1260.
5010.
2420.
13000.
301.
5.04
HD
14700.
ND
ND
NO
0.703
6.51
16.?
11.2
39.3
NO
ND
10.2
8.3
22.4
28.8
152.
ND
270.
ZRD
7.74
3.41
1.17
0.47
0.5
0.45
5.02
2.16
1.39
1.99
2.02
1.25
1.02
11.33
4.7
0.66
0.85
1.3?
1.63
13
:56
30DEC85
AVER/WE
72.5
1270.
5050.
2420.
13000.
354.
5.46
KD
14800.
ND
ND
ND
0.712
6.61
16.8
11.1
36.3
ND
ND
9.89
8.45
22.8
29.1
158.
ND
272.
ZRO
10.85
2.64
-2.85
0.8
-1.71
-30.64
-10.74
0.98
-1.3
-1.1
3.7
0.41
14.95
-5.94
1.05
-3.29
-2.58
-6.89
-2.83
14:
04
30BEC85
AVERAGE
57.4
961.
5280.
2110.
12500.
29?.
5.55
1.49
12400.
NO
NO
ND
0.629
6.62
16.2
10.5
30.7
ND
ND
13.
6.95
23.9
26.2
163.
ND
210.
ZRD
7.83
-14.87
49.55
0.79
-7.06
-2.43
6.85
-33.75
-10.
-12.4
1.12
-5.79
-3.72
-0.33
-11.17
-0,94
23.
-14,82
-8.64
-12.62
14
:os
30DEC8S
AVERAGE
57.4
1080.
4030.
2120.
13000.
292.
5.17
1.67
13300.
ND
NO
ND
0.679
6.65
17,4
10.7
31.2
ND
ND
13.9
7.34
21.8
28.6
168.
ND
236.
ZRD
-8.08
-9.26
-2.84
-1.76
-1.18
7.51
7.11
8.66
-5.04
-3.22
-2.08
-7.36
-1.01
-2.88
-2.84
-9.73
-6.04
-3.07
1.61
-11.22
AVERAGE . BtD
(H) * MEASUREMENT MTA
(A) * ANALYSIS DATA
ND » LESS THAN LOB
ffO * PERCENT RELATIVE DIFFERENCE
EPA/RSKERL/ABAtlX
-------
PRECISION REPORT
DUPLICATE ANALYSIS DATA
ADURPT.LSTJ637
PROJECT: INGERS SITE SAMPLES COLLECTED 9/20/93
P181.8
CONCENTRATION IN N6/KB UET HT.
SAMP
NO
TIME
BATE
ELHT
NA
K
CA
M6
FE
MM
CO
NO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
AG
TL
PB
LI
SR
V
BA
B
TI
180 (H)
PLOT
14212
1NOV8S
AVERAGE
103.
1130.
4430.
2310.
13400.-
441.
5.61
ND
14000.
NO
ND
ND
0.761
7.13
18.1
12.1
33.3
ND
ND
10.5
7.91
23.1
31.3
170.
3.27
278.
1 CONTROL
»
ZRO
9.93
10.28
1,73
0.32
0.12
1.52
0.9
1.58
6.4
1.24
0.96
0.26
1.1
1.11
6.61
0.21
2.32
0.77
7.42
2.05
180 (A)
0-6§ PLOT
13!45
1NOV85
AVERAGE
105.
1120.
4090.
2260.
13500.
382.
5.03
NO
13900.
ND
ND
ND
0.747
6.9
17.6
12.
32.8
ND
ND
1 CONTROL
184
(A)
0-6' GROUP 2 PLOT 2
0-6
14i
•
02
1NOV85
ZRD
6.59
12.35
14.8
5.04
-0.22
28.82
24.35
2.9?
10.16
7.96
7.13
1.5
1.87
11.3 -15.89
7.87
21.7
30.2
164,
7.74
12.69
5.31
8.32
5.08. -66.27
271.
3.68
AVERA6E
97.1
966.
3710.
1950.
11500.
221,
4.04
5.6
12100.
NO
ND
ND
0.623
7.51
18.6
10.4
35.5
ND
ND
15,3
7.1
21.8
25.8
186.
3.53
205.
ZRO
6.81
14.21
-17.65
3.47
2.23
-36.1
-9.21
-7.56
8.63
2.73
-0.09
5.08
-6.96
-15.8
-9.1
10,11
-13.27
5,34
-6.53
55.15
27,54
188
PLOT
(A)
0-6'
14:09
1NOV85
AVERAGE
94.7
967.
3610.
2050.
12200.
330.
4.42
ND
13400.
ND
ND
ND
0.67
6.43
16.6
10.1
30,2
ND
ND
10.
7.26
20.
27.5
141.
2.27
237.
ZRD
1.38
1.26
13.84
-0.07
3.16
84.82
38.47
3.99
1.82
0.74
4.57
3.66
1.33
27.81
5.06
15.56
-0.3
8.33
-63.25
1.33
AVERAGE ZRD
(H) * MEASUREMENT OATA
(A) * ANALYSIS DATA •
ND * LESS THAN LOT
ZKB * PERCENT RELATIVE DIFFERENCE
EPA/RSKERL/ADAtOK
-------
PRECISION REPORT
DUPLICATE ANALYSIS DATA
AOURPT.LSTU23
PROJECT: INBERSAQC
DIGESTED EXTRACTS FROM CONTRACT LAJ
CONCENTRATION IN H6/K6 VET VT,
SAW-
NO
TINE
DATE
ELNT
NA
K
CA
H6
FE
HN
CO
NO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
AG
TL
PB
LI
SR
V
BA
B
TI
1247 -- HEASUREMENT DATA
(A) - ANALYSIS DATA
ND = LESS THAN LOB
ZRD ' PERCENT RELATIVE DIFFERENCE
EPA/RSKERL/ABAtOK
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR:
LIST.LSTI2247
PROJECT: OLD INGERS SAMPLES COLLECTED 4/15/86
P9.13
CONCENTRATION INI M6/K6 DRY UT.
DATE 22MAY86 STDV 22MAY86
TIME 18108 +/- 1811
TA6.NO. 3054 3054 3054BUP
ELEMENT VALUE VALUE VALUE
22MAYB6
18H1
3055
VALUE
STDV
f/-
3055
VALUE
VAUC
VALUE
NA
K
CA
M6
FE
HN
CO
HO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
AC
TL
PB
LI
SR
V
BA
B
TI
124,
1410.
4130.
2310.
15600.
311.
5.03
<1.
18000.
<22.
<12.
<.2
0.91
8.1
20.4
11.5
35.0
<.9
<5.
13.7
9.9
23.1
38.0
175.
4.6
349.
13.
140.
410.
•230.
1500.
30.
0,97
1.5
1800.
22.
12.
0.28
0.30
2.4
2.0
1.2
3.8
0.97
5.4
5.7
1.0
2.3
4.6
17.
1.5
34.
108.
989.
3990.
2190.
15200.
279.
4.4
<1.
15700.
<19.
<11.
<.2
0,79
7.8
19.5
11.8
36.2
<1.
<4.
15,2
8.30
20.8
33.2
170.
3.7
260.
11.
99.
400.
220.
1500.
27.
1.0
1.3
1500.
19.
11,
0.29
0.30
2.4
1.9
1.2
3.9
1.0
4.8
5.3
0.98
2.0
4.1
17.
1.3
26.
107.
1140.
4000.
2290.
15000.
271.
5.34
<1.
16100.
<19.
<11.
<,2
0,82
7.6
19.4
11.6
35.0
<.9
<4.
15.9
8.60
21.5
35,8
166.
5.1
298.
11.
110.
400.
230.
1500.
26.
0.95
1.4
1600.
19.
11.
0.28
0.29
2.3
1.9
1.2
3.8
0.96
4.9
5.4
0.94
2.1
4.3
16.
1.3
29.
< VALUE«LINIT OF DETECTION DETERMINE! BY INSTRUMENT SENSTiSAHPLE DIL» AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SI6NIFICANT DIGITS
EPA/RSKERL/ADAtOK
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR.
LIST,LST?2247
PROJECT: OLD INGERS SAMPLES COLLECTED 4/15/86
P9.13
CONCENTRATION IN! NBAS DRY YT,
»TE
[ME
tt.NO.
LEHENT
NA
K
CA
MS
FE
HN
CO
HO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
AG
TL
PB
LI
SR
V
BA
B
TI
22MAY86
18102
3051
VALUE
119.
1020.
5060.
2440.
14900.
495.
6.93
1.4
13700.
<17.
<11.
0.27
0.83
8.0
18.3
12.5
37.5
<,9
<4.
13.9
8.41
25.2
32.8
202.
3.8
266.
STDV
t/-
3051
VALUE
12.
100.
500.
240.
1400.
49,
0.91
1.2
1300.
17.
11.
0.27
0,27
2.3
1.8
1.3
4.0
0.92
4.3
4.8
0.90
2.5
4.0
20,
1.2
26.
22MAY86
18J04
3052
VALUE
153.
1620.
5150.
2650.
17200.
545.
8.06
3.5
18900.
<23.
<13.
<.2
1.06
10.5
24.7
14.4
41.3
<»9
<5.
20,2
11.3
26.9
41,9
242.
6.6
367.
STDV
+/-
3052
VALUE
16.
160.
510,
260.
1700.
54.
0,96
1.8
1800.
23.
13.
0.28
0.29
2.6
2.4
1.5
4.5
0.96
5.6
6.5
1.1
2,6
5.0
24.
1.5
36.
22MAY86
is:os
3052D
VALUE
129.
1050.
4160.
2450.
17500.
824.
10.4
3.2
15200.
<18.
<13.
<.2
1.08
11.6
20,8
14.8
40.9
<.9
<4«
22.0
9.48
22.4
37.1
258,
2.9
188.
STDV
*/-
2052DUP
VALUE
13.
100.
410.
240.
1700.
81.
1,1
1.5
1500.
18.
13.
0.28
0.28
2.7
2.0
1.5
4.4
0.96
4,7
5.9
0.95
2.2
4.5
25.
1.2
18.
22NAY86
18107
3053
VALUE
125.
1130.
4620.
2330.
13900.
354.
5.83
1.4
14800.
<18»
<11.
<.2
0.78
7.8
19.2
11.6
35.4
<.9
<4.
14.2
8.93
23.9
31.9
181,
4.6
292,
STDV
f/-
3053
VALUE
13.
110,
460,
230,
1300.
35.
0.98
1,3
1400.
18,
11,
0.29
0.30
2,2
1,9
1,2
3.8
0.98
4.6
5,0
0.97
2.3
3.9
IB.
1.3
29.
< VALUE«LI«T OF DETECTION DETERMINED BY INSTRUMENT SENSTiSANPLE OILf AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
EPA/R$KERL/ADA»OK
-------
ELEMENT*! CONSTITUENTS ANALYSIS BY ICAP
FOR*
LIST.LSTJ2247
PROJECT: OLB INGERS SAMPLES COLLECTED vis/86
P9.13
CONCENTRATION IN! KG/KG DRY «T,
DATE
TIME
TA6.NO.
ELEMENT
NA
K
CA
H6
FE
MN
CO
NO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
AG
TL
PB
LI
SR
V
BA
B
TI
* ••*• ii^_i
22HAY86
17:53
3047
VALUE
130.
1340.
4420.
2580.
17100.
552.
7.31
<1.
18500.
<22.
<13.
<.2
1.01
9.0
21.7
13.7
36.9
<",?
<5.
15.5
9.91
23.6
42,2
223.
6.1
359.
STDV
*/-
3047
VALUE
13.
130.
440.
250.
1700.
54.
0.98
1.6
1800.
22.
13.
0.28
0.30
2.6
2.1
1.4
4.0
0.99
5.5
6.0
0.99
2.3
5.0
22.
1.5
35.
22MAY86
17134
3048
VALUE
164.
1450.
5660.
2680.
15800.
281.
5.10
3.4
16400.
<20.
0.89
9.1
22.4
13.2
39.6
16.3
10.0
28.7
36.6
195.
5.1
320.
STJV
*/-
3048
VALUE
17.
140.
560.
260.
1500.
27.
0.91
1.6
1600.
20.
12.
0.26
0.28
2.4
2.2
1.4
4.3
0.91
5.0
5.6
1.0
2.8
4.4
19.
1.4
32.
22MAY86
17J59
3049
VALUE
161.
1330.
7290.
2560.
13900.
236.
4.64
2.0
15500.
0.79
7.8
20.2
11.7
37.4
12.3
9.64
39.0
32.2
179,
6.8
298.
DETECTION KTERHINED IT INSTRUMENT SENSTrSAHPLE DILi AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIBITS
STW
*/-
3049
VALUE
16.
130.
720.
250.
1300.
23.
0.92
1.4
1500.
18.
10.
0.27
0.28
2.2
2.0
1.2
4.0
0.92
4.8
5.0
0.97
3.9
3.9
18.
1.3
29.
•w • i ITI~ m rrm i~i tn
22HAY86
ia:oi
3050
VALUE
149.
1530.
4760.
2540.
14800.
273.
4.86
1.5
17000.
<20.
<11.
<.2
0.86
7.4
20.5
11.4
35.0
<,9
<5.
12.5
9.9
26,2
35.8
170.
11.3
388.
STDV
*/-
3050
VALUE
15.
150.
470.
250.
1400.
26.
0.99
1.5
1700.
20.
11.
0.29
0.31
2.3
2.0
1.2
3.8
0.99
5.2
5.4
1.0
2.6
4.3
17.
1.7
38.
EPA/RSKERL/ADArOK
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR:
LIST.LSTI2247
PROJECT: OLB INGERS SAMPLES COLLECTED 4/15/86
P?.13
CONCENTRATION IN: MB/KB DRY WT,
BATE
TIME
TAG, NO.
ELEMENT
NA
K
CA
M6
FE
m
CO
NO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
22MAY86
17:47
3043
VALUE
108.
1020.
3620.
2190.
144CO.
279.
4.8
<1.
14500.
<18»
<11.
<.2
0.74
6.9
17.5
10.4
32.1
<1.
<4.
13.7
7.73
19.1
31,0
158.
3.2
237.
STDV
t/-
3043
VALUE
11.
100.
360.
220.
1400.
27.
1.0
1.2
1400.
18.
11.
0.29
0.30
2.3
1.7
1.1
3.5
1.0
4,5
4.9
0.99
1.9
3.8
IS.
1.3
23.
22MAY86
17!48
3044
VALUE
174.
1720.
4900.
2690.
15300.
294.
4.96
<1.
18400.
<22.
<12.
<»2
0.91
8.5
22.9
12.7
37.3
<.8
<5.
11.7
11,4
26.7
37.1
183.
6.2
383.
STDV
f/-
3044
VALUE
18.
170.
490.
270.
1500.
29.
0.88
1.5
1800.
22.
12.
0.25
0.27
2.3
2.2
1.3
4.0
0,87
5.5
5.6
1,1
2,6
4.5
18.
1.5
38.
22MAY86
17!5
3045
VALUE
132.
1280.
5010.
2590.
16900.
300.
4.6
<1.
16800.
<20.
<13.
<»2
0.89
8.0
20.6
12.8
37.7
<1.
<5.
13.2
9.34
24.7
40.4
171.
6.2
338.
STDV
+/-
3045
VALUE
14.
120.
500.
260.
1600.
29.
1.0
1.4
1600.
20.
13.
0.29
0.31
2.6
2.0
1.3
4.1
1.0
5,2
5.5
0.99
2.4
4.9
17.
1.5
'33.
22HAYB6
17J51
3046
VALUE
137.
1340.
3800.
2640.
17000.
484.
6.86
<1.
17900.
<21.
<13.
<.2
0.94
7.9
21.3
13.2
35.0
<,9
<5.
15.0
10.5
21.6
38.7
192.
6.2
349,
STDV
*/-
3046
VALUE
14.
130.
380.
260.
1700.
47.
0.95
1.5
1700*
21.
13.
0.27
0.29
2.6
2.1
1.4
3.8
0.96
5.5
5.9
1.0
2.1
4.7
19.
1.5
34.
< VALUE«LINIT OF DETECTION DETERMINED BY INSTRUMENT SENSTiSAHPLE DILi AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SI6NIFICANT BI6ITS
EPA/RSKERL/ADA.OK
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR:
LIST.LSTJ2247
PROJECT: OLD INGERS SAMPLES COLLECTED 4/is/w
P9.13
CONCENTRATION IN*. M6/K6 DRY UT.
DATE 22Mm STDV
TIME 17M */-
TA6.NO. 3040 3040
ELEMENT VALUE VALUE
22MAY86
17J42
3041
VALUE
STDV
*/-
3041
VALUE
22HAY86
17J43
3042
VALUE
STDV
+/-
3042
VALUE
NA
K
CA
M6
FE
HN
CO
HO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
152.
1400.
4940.
2350.
14000.
227.
3.87
<1,
16500.
<20.
<11.
<.2
0.84
7.5
19.6
11.2
33.8
<,8
<4.
10.8
9.92
26.?
33.3
165.
6.0
337.
15.
140.
490.
230.
1400.
22.
0.81
1.3
1600.
20.
11.
0.23
0.25
2.1
1.9
1.2
3.6
0.81
4.9
5.1
0.99
2.7
4.0
16.
1.3
33.
115.
1000.
4050.
2300.
14500.
260.
5.55
<1.
15800.
<19.
<11.
<.2
0.76
7.3
19.3
10.8
34.6
<.9
<4.
13.0
8,48
20.6
31.0
165.
3.2
256.
12.
100.
400.
230.
1400.
25.
0.99
1.3
1500.
19.
11.
0.29
0.30
2.3
1.9
1.1
3.7
0,99
4.8
5.1
0.97
2.0
3.8
16.
1.3
25.
133.
1270.
5640.
2470.
14600.
241.
4.38
<1.
16300.
<20.
<11.
<.2
0.83
7.3
19.6
11.9
38.2
<.9
<5.
12.1
9.22
28.3
33.8
164.
4.9
331.
14.
120.
560.
240.
1400.
23.
0.97
1.4
1600.
20.
11.
0.28
0.30
2.3
1.9
1.2
4.1
0.97
5.0
5.2
0.95
2.8
4.1
16,
1.4
33.
129.
1200.
6010.
2470,
14500.
240.
4.22
<1.
15700.
<19.
<11.
<.2
0.81
7.1
19.4
11.4
37.3
<.9
<4.
13.1
8.95
28.1
33.0
161.
4.4
317.
13.
120.
600.
240.
1400.
23.
0.99
1.3
1500.
19.
11.
0.29
0.30
2.3
1.9
1.2
4.0
0.99
4.9
5.1
0.97
2.8
4.0
16.
1.4
31.
< VALUE=LIMIT OF DETECTION DETERNINEB BY INSTRUMENT SENSTrSAMPLE DILf AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIBITS
EPA/RSKERL/ADAfOK
-------
ELEMENTAL CONSTITUENTS ANALYSIS IT ICAP
FOR:
LIST.LSTJ2243
PROJECT: OLD INGERS SITE SAMPLES COLLECTED 3/10/86
P13.9
CONCENTRATION IN: K6/K6 DRY «T.
MTE 22NAY86 STDV
TIME 17:26 +/-
rAG.NO. 2487 2487
•LENENT VALUE VALUE
VALUE
VALUE
VALUE
VALUE
m
K
CA
H6
FE
m
CO
MO
AL
AS
SE
CD
DE
CU
CR
NI
ZN
A6
TL
PD
LI
SR
V
BA
D
TI
155.
1660.
5180.
2700.
15400.
395.
5.89
5.3
17700.
<21.
<12.
<.2
0.92
9.7
23.9
13.4
44.1
<.9
<5.
16.1
11.6
29.6
3S.5
242.
5.7
368.
16.
160.
510.
270.
1500.
39.
0.99
1.8
1700.
21.
12.
0.29
0.30
2.4
2.3
1.4
4.7
0.99
5.3
5.8
1.1
2.9
4.3
24.
1.5
36.
144.
1640.
5380.
2690.
16000.
538.
7.9
5.5
17600.
<21,
<12.
<.2
0.93
9.9
24.9
14.6
42.3
<1.
<5.
19.6
• 11.4
28.2
37.4
257.
7.1
366.
15.
160.
530.
270.
1600.
53.
1.0
1.9
1700.
21.
12.
0.29
0.31
2.5
2.4
1.5
4.5
1.0
5.5
6.1
1.1
2.8
4.5
25.
1.5
36.
< VALUE*LIMIT OF DETECTION DETERMINED DY INSTRUMENT SENSTtSAHPLE DILr AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIBNIFICANT DIGITS
EPA/RSKERL/ADA»OK
-------
ELENENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR:
LIST.LSTJ2243
PROJECT: OLD INGERS SITE SAMPLES COLLECTED 3/10/86
P13.9
CONCENTRATION IN! NG/KG DRY HT.
MTE 22MAY86 STDV 22MAY86 STBV 22NAY86
FINE 17J2 t/- 17J22 */- 17:23
FAG.NO. 2483 2483 2484 2484 2485
ELEMENT VALUE VALUE VALUE VALUE VALUE
< VALUE«LINIT OF DETECTION DETERMINED BY INSTRUMENT SENSTiSANPLE DILt AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
STDV
*/-
2486
VALUE
NA
K
CA
M6
FE
HN
CO
MO
AL
AS
SE
CD
BE
CU
CR
HI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
110.
1210.
4440.
2370.
16900.
557.
7.46
<1.
15000.
<18.
<13.
<.2
0.87
7.2
19.0
11.2
34.4
<1.
<4.
12.8
8.46
23.2
33.1
217.
5.5
326.
11.
120.
440.
230.
1600.
55.
0.99
1.3
1500.
18.
13.
0.29
0.30
2.6
1.8
1.2
3.8
1.0
4.8
5.1
0.97
2.3
4.1
21.
1.4
32.
189.
1050.
16500.
2400.
14500.
472.
6.4
<1.
14700.
<18.
<11.
<.2
0.77
8.1
18.0
12.8
34.8
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR:
LIST.LSTJ2237
PROJECT: OLD INGERS SITE SAMPLES COLLECTED 1/22/8*
P9.9
CONCENTRATION IN! MS/KG DRY HT.
DATE 2mm STDV 21MAY86 STDV
TIKE 20U4 */- 20:15 V-
TA6.NO. 1947 1947 1948 1948
ELEMENT VALUE VALUE VALUE VALUE
VALUE
VALUE
NA
K
CA
M6
FE
HN
CO
MO
AL
AS
SE
CD
BF
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
134.
1470.
S210.
2870.
17600,
375.
3.78
<1.
18000.
<22,
<13.
<.2
0.90
8.8
22.7
14.3
S3.0
<,9
<5.
12.7
9.09
26.9
38.5
192.
4.9
381.
14.
140.
520.
280.
1700.
37.
0.94
1.5
1800.
22.
13.
0.28
0.30
2.7
2.2
1.4
5.7
0.96
5.4
5.7
0.93
2.6
4,7
19.
1.5
38.
121.
1100.
6440.
2410.
14500.
545.
8.16
<1.
14900.
<1B.
<11.
<,2
0,82
7.9
19.0
12.7
37.5
<,8
<4.
13.6
7.68
29.5
32.4
187.
3.4
248.
13.
110.
640.
240.
1400.
54.
0.87
1.3
1400.
18.
11.
0.25
0.26
2.2
1.8
1.3
4.1
0.88
4.8
5.0
0.86
2.9
3.9
18.
1.2
24.
164.
981.
19300.
2300.
13200.
330.
5.6
<1.
13800.
<17.
<10.
<.2
0.67
7,4
17,1
11.4
37.3
<1,
<5.
13.1
6.83
80.2
28.9
159.
1,8
239.
t7.
99.
1900.
230.
1300.
32.
1.0
1.2
1300.
17.
10.
0.29
0.30
2.1
1,6
1.2
4.0
1.0
5.7
4.7
0.99
8.0
3.5
16.
1.4
24.
< VALUE*LIHIT OF 1ETECTION BETERMINED BY INSTRUMENT SENSTfSAMPLE DILr AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIBITS
EPA/RSKERLWAfOK
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR!
LIST.LSTI2237
PROJECT: OLD INSERS SITE SAMPLES COLLECTED 1/22/86
P9.9
CONCENTRATION IN! M6/K6 DRY UT.
DATE 21MAY86 STDV 21NAY86 STDV 21HAY86 STDV 21MAY86 STDV
TIME 20J08 t/- 20J09 f/- 20M1 +/- 20J12 +/-
TA6.NO. 1943 1943 1944 1944 1945 1945 1946 1946
ELEMENT VALUE VALUE VALUE VALUE VALUE VALUE VALUE VALUE
MA
K
CA
H6
FE
MN
CO
MO
AL
AS
SE
CO
BE
cu
CR
HI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
134.
1440.
4740.
2850.
15100.
290.
4,9
<1.
16600.
<20.
<11,
<.3
0.81
8.2
21.1
12.5
38.4
<1.
<5.
11.1
8.6
24.8
34.4
170.
5.5
317.
14.
140.
470.
280.
1500.
28.
1.0
1.4
1600.
20.
11.
0.30
0.31
2.4
2.0
1.3
4.2
1.0
5.1
5.2
1.0
2.4
4.1
17.
1.4
31.
105.
1070.
4240.
2380.
12400.
254.
4.9
<1.
13700.
<16.
<9.
<.2
0.71
6.8
16.4
11.3
34.4
<1.
<4.
12.0
7.05
21.3
28.5
145.
2.0
261.
11.
100.
420.
240.
1200.
25.
1.0
1.1
1300.
16.
9.9
0.29
0.30
2.0
1.6
1.1
3.7
1.0
4.0
4.5
0,99
2.1
3.4
14.
1.3
26.
125.
1260.
4610.
2490.
14700.
333.
5.3
4.8
15600.
<1*.
<11.
<.2
0.91
9.0
21.9
12.1
41.5
<1.
<4.
16.4
8.51
24.5
32.3
220.
6.5
264.
13.
120.
460.
250.
1400.
32.
1.0
1.6
1500.
18.
11.
0.29
0.30
2.3
2.1
1.2
4.5
1.0
4.6
5.4
0.99
2.4
3.9
22.
1.3
26.
144.
1440.
7740.
2540.
14500.
243.
5.2
3.7
16700.
<20.
<11.
<.3
0.92
9.1
22.5
11.9
41.6
<1.
<5.
15.7
8.9
42.6
32.3
214.
1.6
279.
IS.
140.
770.
250.
1400.
23.
1.0
1.6
1600.
20.
11.
0.30
0.31
2.3
2.2
1.2
4.5
1.0
5.2
5.6
1.0
4.2
3.9
21.
1.4
28.
< VALUE4.IMIT OF DETECTION DETERMINED IY INSTRUMENT SENSTtSAMPLE DILt AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
EPA/RSKERL/ADAiOK
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR:
LIST.LSTI2237
PROJECT: on INGERS SITE SAMPLES COLLECTED 1/22/8*
P9.9
CONCENTRATION IN! H6/KS DRY IfT.
STDV
*/-
1940
VALUE
14.
110.
920.
220.
1300.
31.
0.94
1.3
1500.
18.
10.
0.28
0.28
2.1
1.7
1.1
3.9
0.95
4.9
5.0
0,93
3.4
3.7
17.
1.2
23.
DATE
TIME
TA6.NO.
ELEMENT
NA
K
CA
M6
FE
MM
CO
HO
AL
AS
SE
CD
BE
CD
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
21HAY86
20:02
1940
VALUE
139.
1120,
9200.
2230.
13500.
318.
5.12
<1.
15400.
<18.
<10.
<,2
0.78
7.2
18.0
11.2
35.7
<,9
<4.
12.7
7,76
34.9
30,9
176.
4.4
233.
21MAY86
20:03
1940DUP
VALUE
127.
1380,
4510.
2340,
14200.
298.
4.5
<1.
16800.
<20.
<11.
<.3
0.86
7,6
19.7
11.5
36.3
<1.
<4.
12.5
8.3
24.5
34,7
179.
10.0
312.
STDV
V-
1940DUP
VALUE
13.
130,
450.
230.
1400.
29.
1.0
1.4
1600.
20.
11.
0.30
0.31
2.2
1.9
1.2
3.9
1.0
4.9
5.3
1.0
2.4
4,1
IB.
1.5
31.
21NAY86
20!05
1941
VALUE
149.
1450.
4240.
2550.
14000.
227.
4.22
2.6
16300,
0.81
8.1
22.3
11.8
39,1
12.9
8.55
24.4
32,5
187.
7.6
332.
< VALUE*LIMIT OF DETECTION DETERMINES BY INSTRUMENT SENSTrSAHPLE DILi AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
STDV
\l-
1941
VALUE
15.
140.
420.
250.
1400.
22.
0.98
1.5
1600.
19.
11.
0.28
0.30
2.2
2.2
1.2
4.2
0.98
4.9
5.2
0.96
2.4
3.9
18.
1.4
33.
21MAY86
20:06
1942
VALUE
116.
1350.
4320.
2410.
15300.
277.
4,7
<1.
16800.
<20.
<12.
<.2
0.83
7.2
20.9
12.1
34.0
<1.
<5.
13.2
8.00
23.4
36.1
165.
7.9
330.
STDV
*/-
1942
VALUE
12.
130.
430.
240.
1500.
27.
1.0
1.4
1600.
20.
12.
0.29
0,31
2.4
2.0
1,2
3.7
1.0
5.1
5.4
0,99
2.3
4,3
16.
1,4
33.
EPA/RSKERL/ADAfOK
-------
ELEHENTM. CONSTITUENTS ANALYSIS BY ICAP
FOR:
LIST.LSTI2237
PROJECT: OLD INGERS SITE SAMPLES COLLECTED 1/22/86
P9.9
CONCENTRATION INI KG/KG DRY MT.
MTE 21MAY86 STDV 21HAY86 STDV 21MAY86 STDV
TIME 19551 */- 19:53 f/- 19154 */-
FAG.NO. 1934 1936 1937 1937 1938 1938
ELEMENT VALUE VALUE VALUE VALUE VALUE VALUE
NA
K
CA
N6
FE
m
CO
m
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
118.
1260.
4300.
2370.
HIM.
269.
4.82
<1.
15800.
<19.
<11.
<.2
0.79
7.0
18.5
11.3
34.4
<.9
<4.
11.9
7,73
21.7
32.3
165.
3.0
300.
12.
120.
430.
230.
1400.
26.
0.90
1.3
1500.
19.
11.
0.26
0.27
2.2
1.8
1.1
3.7
0.90
4.7
5.0
0.88
2.1
3.9
16.
1.3
30.
148.
1260.
5290.
2530.
14200.
277.
5.07
<1.
15700.
<19.
<11.
<.2
0.80
7.5
20.3
12.6
37.9
<.8
<4.
10.5
8.06
26.8
31.5
167.
3.4
256. .
15.
120.
530.
250.
1400.
27.
0.86
1.3
1500.
19.
11.
0.25
0.26
2.2
2.0
1.3
4.1
0.86
4.6
4.9
0.84
2.6
3.8
16.
1.2
25.
126.
1450.
4840.
2650.
15900.
280.
5.1
<1.
17400.
<21.
<12.
<.3
0.84
8,0
22.1
14.8
39.1
<1.
<5.
11.2
8.8
26.5
36.5
172.
6.0
374.
13.
140.
480.
260.
1500.
27.
1.0
1.5
1700.
21.
12.
0.30
0.31
2.5
2.1
1.5
4.2
1.0
5.3
5.4
1.0
2.6
4.4
17.
1.6
37.
227.
1520.
18600.
2390.
13700.
300.
5.6
<1.
16900.
<20.
<10.
<.3
0.79
7.2
19.3
10.9
34.4
<1.
<6.
11.2
8.6
72.8
33.4
175.
14.8
344.
23.
150.
1800.
240.
1300.
29,
1.0
1.4
1600.
20.
10.
0.30
0.31
2.2
1.9
1.1
3.7
1.0
6.2
5.3
1.0
7.2
4.0
17.
2.1
34.
< VALUE=LIMIT OF DETECTION DETERMINED DY INSTRUMENT SENSTiSAMPLE DILi AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
EPA/RSXERL/ADAfOK
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR!
LIST.LSTJ2237
PROJECT: OLD INBERS SITE SAMPLES COLLECTED 1/227*6
P9.9
CONCENTRATION IN! M8/K6 DRY NT.
DATE
TIME
TA6.M.
ELEMENT
NA
K
CA
H6
FE
m
CO
MO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
21NAY86
19M5
1932
VALUE
• 129.
1020.
3910.
2370.
13100.
235.
4.55
<1.
14200.
<17.
<10.
<.2
0.66
6.7
17.7
10.7
32.4
<,8
<4.
10,1
6.12
20,4
27.5
154.
8.5
242.
STDV
+/-
1932
VALUE
13.
100.
390.
230.
1300.
23.
0.89
1.2
1400.
17.
10.
0.26
0.27
2.0
1.7
1.1
3.5
0.89
4.3
4.5
0.87
2.0
3.3
IS.
1.2
24.
21MAY86
19J47
1933
VALUE
185.
1440.
9350.
2590.
14900.
293.
5.17
17100.
0.79
7.9
21,7
13.1
55.9
10.9
8.64
42.1
34.2
183.
4.6
363.
STDV
*/-
1933
VALUE
19.
140.
930.
260.
1400.
28.
0.99
1.4
1700.
21.
11.
0.29
0.30
2.3
2.1
1.3
5,9
0.99
5.4
5.3
0.96
4.2
4.1
18.
1.5
36.
21MAY86
19!48
1934
VALUE
145.
1590.
7450.
2610.
15400.
372.
5.8
18300.
<22.
0.90
8.0
21.4
12.8
36.4
13.3
8.99
35.6
37.4
198.
5.3
382.
STDV
*/-
1934
VALUE
IS.
160.
740.
260.
1500.
36.
1.0
1.5
1800.
22.
12.
0.29
0.31
2.4
2.1
1.3
4.0
1.0
5.5
5.8
0.98
3.5
4.5
19.
1.6
38.
21HAY86
19:5
1935
VALUE
156.
1630.
4500.
2570.
17400.
366.
6.6
<1.
22000.
<27.
<13.
<.2
1.03
8.9
24.4
13.8
37.7
<1,
<6.
12.6
9,79
27.5
40,8
. 200.
7.8
385.
< VALUE»LIMIT OF DETECTION DETERMINE BY INSTRUMENT SENST,SAMPLE DILi AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT BI6ITS
STDV
*/-
1935
VALUE
16.
160.
450.
250.
1700.
36.
1.0
1.8
2200.
27.
13.
0.29
0.31
2.7
2.4
1.4
4.2
1.0
6.3
6.6
0.99
2.7
4.9
20.
1.6
38.
EPA/RSKERL/ADAfOK
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR:
LIST.LSTI2237
PROJECT: OLD IMBERS SITE SAMPLES COLLECTO 1/22/8*
P9.9
CONCENTRATION IN! H6/K6 DRY WT.
BATE
TINE
TA6.NO.
ELEMENT
MA
K
CA
MS
FE
m
CO
MO
AL
AS
SE
CD
BE
OJ
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
j IIAI ttf-A
21HAYB6
19139
1929
VALUE
146.
1250.
6340.
2640.
14700.
349.
6.14
<1.
15900.
<19.
<11.
<.2
0.83
7.9
18.9
12,8
37.4
<.9
<4.
10.7
8.38
28.0
31.4
170,
24.8
278.
VMW ftf tf il'M
STDV
+/-
1929
VALUE
15.
120.
630.
260.
1400.
34.
0.91
1.3
1500.
19.
11.
0.26
0.27
2.3
1.8
1.3
4.1
0.91
4.8
5.0
0.39
2.8
3.8
17.
2.7
27.
TTHM A^WVUCVUf"
21MAY86
19M1
1929DUP
VALUE
121.
1540.
5670.
2740.
15200.
259.
4.60
<1.
17600.
<21.
<11.
<,2
0.85
7,6
21.1
12.4
36.5
<.9
<5.
9.8
9.05
26.0
34.6
177.
9.1
357.
Ik •«• • IM* iiMMl^lil
STDV
+/-
1929DUP
VALUE
13.
150.
560.
270.
1500.
25.
0.95
1.4
1700.
21.
11*
0.28
0.29
2.3
2.0
1.3
4.0
0.95
5.2
5.3
0.93
2.5
4.2
17.
1.5
35.
r Mf*UMV MAMMA f
21MAYB6
19!42
1930
VALUE
122.
1410.
4240.
2510.
16100.
460.
6.2
<1.
18100.
<22.
<12.
<«2
0.90
8.1
21.4
12.8
36.7
<1.
<5.
12.8
8.53
24.5
37.8
199.
5,4
347,
IkVl AUK UAWh
STDV
+/-
1930 t
VALUE
13.
140.
420.
250.
1600.
45.
1.0
1.5
1800.
22,
12,
0.29
0.31
2.5
2.1
1.3
4.0
1.0
5.3
5.7
0.98
2.4
4.5
19.
1.5
34.
21HAY86
19!44
1931
VALUE
140.
1380.
6670.
2220.
13800.
381.
6.36
<1.
16200.
<20.
<10.
<.2
0.81
7.5
19.0
11.2
32.8
<»9
<4.
13.9
7.87
28.7
34.2
184.
3.4
344.
STDV
\l-
1931
VALUE
15.
140.
660.
220.
1300.
37.
0.99
1.3
1600.
20.
10.
0.29
0.30
2,2
1,8
1.1
3.6
0.99
4.9
5.3
0.97
2.8
4.1
18.
1.5
34.
RESULTS ACCURATE TO 2 SIGNIFICANT DIBITS
EPA/RSKERL/ADAiOK
-------
PROJECT: OLB INGERS SITE SAMPLES
COLLECTED 1/22/84
P9.1
CONCENTRATION IN! M6/K6 DRY HT.
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR:
LIST.LSTI2233
STHV
*/-
1928
VALUE
13.
130.
450.
230.
1300.
23.
0.99
1.3
1500,
19.
10.
0.29
0.30
2.1
2.1
1.2
3.6
0.99
5.0
5.3
0.98
2.3
3.5
15.
1.4
32.
BY INSTRUMENT SENSTtSAHPLE BILi ANB MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT BI6ITS
BATE
TIME
TA6.NO.
ELEMENT
m
K
CA
H6
FE
MN
CO
NO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
AC
TL
PB
LI
SR
V
BA
B
TI
S IftAI IIP_i
7MAYB6
10M3
1927
VALUE
136.
1310.
4870.
2540.
14700.
443.
7.4
<1.
15800.
<19.
14.
<,2
0.80
11.0
21.9
13.9
36.4
<1.
5.5
19.1
7.20
25.2
32.0
175.
5.1
307.
VMW Hf nfrrt
STDV
*/-
1927
VALUE
13.
130.
480.
250.
1400.
43.
1.0
1.3
1500.
19.
11.
0.29
0.30
2.3
2.1
1.4
3.9
1.0
5.2
5.6
0.99
2.5
3.9
17.
1.4
30.
tWMAl fcl'll lfcM»M^
7MAY86
10M4
1928
VALUE
135.
1350.
4590.
2320.
13100.
239.
4.75
<1.
15900.
<19.
13.
<,2
0.77
6.1
21.5
11.8
33.9
<.9
<5.
16.5
7.25
23.8
28.9
152.
3.3
323.
& &M TlMITnillM-
7NAY86
10!46
1928BUP
VALUE
152.
1180.
8300.
2270.
12800.
230.
4.48
<1.
15300.
<18,
12.
<,2
0.70
6.0
20.2
11.3
33.7
<.9
<5.
15.5
7.15
45.1
27.3
149.
5.0
259.
•hVt A&Ht MATM1
STDV
*/-
19280UP
VALUE VALUE
IS.
110.
830.
220.
1200.
22.
0.96
1.3
1500.
IB.
10.
0.28
0.29
2.0
1.9
1.2
3.6
0.96
5.1
5.1
0.94
4.5
3.3
14.
1.3
26.
VALUE
EPA/RSXERL/ADAiOK
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FORt
LIST.LSTJ2233
PROJECT: ou INGERS SITE SAMPLES
COLLECTED 1/22/86
P9.1
CONCENTRATION IN! H6/K6 DRY «T.
CONCENTRATION IN!
DATE
TIME
TA6.NO.
ELEMENT
NA
K
CA
M6
FE
MN
CO
MO
AL
AS
SC
CD
BE
CU
CR
NI
ZN
AG
TL
PB
LI
SR
V
BA
B
TI
* ••&• i^_i <
7HAY86
10:37
1923
VALUE
151.
1300.
5320.
2420.
14600.
531.
8.7
<1.
15700.
<19.
13.
0.51
0.82
6.9
21.4
16.1
36.0
<1.
5.3
17.9
7.57
27.4
31.0
168.
8.1
311.
STDV
\h
1923
VALUE
15.
130.
530.
240.
1400.
52.
1.0
1.3
1500.
19.
11.
0.29
0.30
2.3
2.1
1.6
3.9
1.0
5.1
5.5
0.98
2.7
3.8
16.
1.4
31.
7MAY86
10138
1924
VALUE
131.
1240.
3570.
2220.
13800.
232.
4.62
<1.
16400.
<20.
12.
<,2
0.77
6.4
20.8
12.2
32.2
<.8
5.2
15.0
7.35
20.2
29.2
158.
6.1
277.
fe ^M 9UM4MM m*^\m
STDV
*/-
1924
VALUE
13.
120.
350.
220.
1300.
22.
0.87
1.3
1600.
20.
10.
0.25
0.26
2.1
2.0
1.2
3.5
0.87
5.2
5.4
0.85
2.0
3.6
15.
1.2
27.
V J^VtMt V M A MIU
7MAY86
10!4
1925
VALUE
129.
1440.
4980.
2430.
14400.
232.
4.6
4.8
17100.
<20.
13.
<.2
0.77
8.0
25.7
14.1
40.6
<1.
6.4
21.9
8.06
26.6
30.5
215.
4.3
304.
•hVt AUfh MAVA
STDV
+/-
1925
VALUE
12.
140.
490.
240.
1400.
22.
1.0
1.7
1700.
20.
11.
0.29
0.31
2.3
2.5
1.4
4.3
1.0
5.5
6.1
0.99
2.6
3.7
21.
1.4
30.
7HAY86
10M1
1926
VALUE
148.
1420.
5180.
2560.
14400.
295.
5.81
<1.
16600.
<20.
13.
<.2
0.83
6.7
21.7
12.0
37.8
<»9
<5.
17.1
7.80
25.2
31.4
170.
6.0
316.
< VALUE-LIMIT OF DETECTION DETERMINED BY INSTRUMENT SENSTtSAHPLE DIL» AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
STDV
f/-
1926
VALUE
14.
140.
510.
250.
1400.
29.
0.90
1.4
1600.
20.
11.
0.26
0.27
2.2
2.1
1.2
4.0
0.90
5.3
5.6
0.88
2.5
3.8
17.
l.*4
31.
EPA/RSKERL/AOAiOK
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR:
LIST.LST52233
PROJECT: OLD INSERS SITE SAMPLES
COLLECTED 1/22/86
P9.1
CONCENTRATION IN! N6/K6 DRY VT.
DATE
TIME
TA6.NO.
ELEMENT
NA
K
CA
MS
FE
HN
CO
HO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
7NAY86
10:28
1919
VALUE
135.
11BO.
4090.
2490.
14100.
407.
6.56
<1.
14700.
<17.
11.
0,37
0.75
6.5
18.9
13.0
34.0
<.9
5,0
15.9
7.37
21.1
28.5
171.
3.2
236.
STDV
+/-
1919
VALUE
13.
110.
400.
250.
1400.
40.
0.95
1.2
1400.
17.
11.
0.28
0.28
2.2
1.8
1.3
3.6
0.95
4.8
5.1
0.93
2.0
3.5
17.
1.2
23.
7HAY86
10:3
1920
VALUE
160.
1610.
5450.
2840.
14300.
250.
4.87
<1.
17300.
<21.
12.
<.2
0.82
6.9
28.4
12.9
52.6
<.8
<5.
16.2
8.35
27.1
30.7
167.
5.3
314.
STW
*/-
1920
VALUE
16.
160.
540.
280.
1400.
24.
0.87
1.4
1700.
21.
11.
0.25
0.27
2.2
2.8
1.3
5.5
0.87
5.5
5.7
0.85
2.7
3.8
16.
1.3
31.
7MAY86
10:31
1921
VALUE
159.
1620.
7730.
2720.
15700.
289.
5.51
2.3
18400.
<22.
14.
<.2
0.93
8.0
26.9
13.9
38.9
<.9
<5.
18.0
8.95
38.1
34.0
195.
6.1
342.
STDV
*/-
1921
VALUE
15.
160.
770.
270.
1500.
28.
0.97
1.6
1800.
22.
12.
0.28
0.29
2.4
2.6
1.4
4.2
0.97
5.8
6.1
0.94
3.8
4.2
19.
1.5
34.
THAW
10:34
1922
VALUE
147.
1550.
4670.
2500.
14200.
243.
4.38
<1.
17500.
<21.
12.
0.36
0.84
6.8
22.9
13.5
35.1
<.8
5.6
15.7
8.23
26.5
31.2
165.
6.7
354.
STDV
*/-
1922
VALUE
14.
150.
460.
250.
1400.
23.
0.89
1.4
1700.
21.
11.
0.26
0.27
2.2
2.2
1.4
3.8
0.89
5.5
5.7
0.87
2.6
3,8
16.
1.4
35.
< VALUE«UNIT OF DETECTION DETERMINED BY INSTRUMENT SENSTiSAMPLE OIL. AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
EPA/RSKERL/ADAtOK
-------
PROJECT: ou DKJERS SITE SAMPLES
COLLECTED 1/22/84
CONCENTRATION IN: KG/KG DRY BT.
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR:
LIST.LSTJ2233
DATE
TIME
TA6.NO.
ELEMENT
NA
K
CA
H6
FE
HN
CO
HO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B.
TI
7MAY86
10:21
1916
VALUE
140.
1200.
5540.
2330.
12900.
237.
4.56
<1.
15000.
<18.
11.
<.2
0.75
6.3
17,4
10.9
32.8
<<8
<4.
13.4
7.31
27,4
27.1
147.
5.4
275.
STDV
•f/-
1916
VALUE
14.
120.
550.
230.
1200.
23.
0,88
1,2
1500.
18.
10.
0.26
0.27
2.0
1.7
1.1
3.5
0.88
4.8
4.9
0,86
2,7
3.3
14.
1.2
27.
7NAY86
10:23
1917
VALUE
156.
1310.
4500.
2540.
14400.
259.
4.8
<1,
16300.
<20.
13.
<.2
0.77
6.5
21.5
12.1
35.2
<1.
<5.
14.8
7.69
23.6
30.0
156.
7.4
305.
STDV
*/-
1917
VALUE
15.
130.
450.
250.
1400.
25.
1.0
1.4
1600.
20.
11.
0.29
0.30
2.3
2.1
1.2
3.8
1.0
5.1
5.4
0.99
2.3
3.7
IS.
1.4
30.
7NAY86
10:24
1917DUP
VALUE
166.
1470.
4330.
2630.
14600.
231.
4.79
<1»
17000.
<20.
15.
<.2
0.87
7.0
22.0
12.4
35.6
<.9
<5.
15.0
8.23
24.1
30.7
163.
7.2
325.
STDV
#/-
1917DUP
VALUE
16.
140.
430.
260.
1400.
22.
0.98
1.4
1700.
20.
11.
0.28
0.30
2.3
2.1
1.3
3.8
0.98
5.4
5.5
0.96
2.4
3.8
16.
1.4
32.
7HAY86
10126
1918
VALUE
164.
1380.
5000.
2390.
13600.
210.
4.41
<1»
16000.
<19.
12.
<.2
0.79
6.6
21,3
12.9
35.3
<.9
<5.
13.5
7,93
26,1
29,0
151.
7,5
320.
STDV
*/-
1918
VALUE
16.
130.
500.
260.
1300.
20.
0.96
1.3
1600.
19,
10.
0.28
0.29
2,1
2.1
1.3
3.8
0.96
5.0
5.2
0.94
2.6
3,6
15.
1,4
32.
< VALUE*IMIT OF DETECTION BETERMINED BY INSTRUMENT SENSTiSAMPLE DILi AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SI6NIFICANT DIBITS
EPA/RSKERL/ADA.OK
-------
PROJECT: OLD INGERS SITE SAMPLES
COLLECTED 1/22/84
P9.1
CONCENTRATION INI M6/K6 DRY VT.
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR:
LIST.LSTI2233
DATE
TIME
TA6.NO.
ELEMENT
NA
K
CA
M6
FE
MN
CO
MO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
7HAY86
10:i
1912
VALUE
148.
1360.
4300.
2300.
14604.
215.
3.5
<1.
18700.
<22.
13.
<.3
0.79
7.3
22.9
12.1
34.4
<1»
<5.
14.6
8.6
25.5
30.6
172.
5.9
291.
STDV
*/-
1912
VALUE
14,
130.
430.
230.
1400.
21.
1.0
1.5
1800.
22.
11.
0.30
0.31
2.3
2.2
1.3
3.7
1.0
5.8
5.9
1.0
2.5
3.8
17,
1.4
29.
7MAY86
10:13
1913
VALUE
152.
1170.
5250.
2450.
14000.
220.
4.18
<1.
15200.
<18.
14.
0.27
0.71
6.5
21.1
11.4
35.4
<«9
<4.
14.7
7.55
27.0
27.0
149.
4.8
225.
STDV
*/-
1913
VALUE
15.
110.
520.
240.
1400,
21.
0.91
1.2
1500.
18.
11.
0.27
0,27
2.2
2.0
1.2
3.8
0.92
4.8
5.1
0.90
2.6
3.4
15.
1.2
22.
7MAY86
10! 15
1914
VALUE
166.
1470.
6770.
2410.
14600.
328.
5.57
<1.
17800.
<21.
14.
<»2
0,83
7.5
23.7
13.0
40.3
<»9
<5.
16.5
8.04
34.0
32.8
180.
7.4
328.
STDV
+/-
1914
VALUE
16.
140.
670.
240.
1400,
32.
0.96
1.5
1700.
21.
11.
0.28
0.29
2.3
2.3
1.3
4.3
0.96
5.6
5.8
0.94
3.3
4.0
18.
1.4.
32.
7MAY86
10I17
1915
VALUE
165,
1530.
5950.
2310.
16300.
364,
6.01
<1.
18400.
<22.
14.
0.27
0.93
7.8
23.7
12.6
35.4
<»8
<5.
18.3
8.59
32.8
36.0
208.
9.5
339.
STDV
*/-
1915
VALUE
16.
150.
590.
230.
1600.
35.
0,88
1.5
1800.
22.
12.
0.25
0.27
2.5
2.3
1.3
3.8
0.89
5.9
6.2
0.86
3.2
4.4
20,
1.4
33.
< VALUE*UNIT OF DETECTION DETERMINED BY INSTRUMENT SENSTiSAHPLE DIL» ANB MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
EPA/RSKERL/ABAiOK
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR:
LIST.LSTJ2233
PROJECT: OLD INSERS SITE SAMPLES
COLLECTED 1/22/86
w.i
CONCENTRATION IN: M6/K6 DRY (IT.
DATE 7MAY86 STDV
TIME 10: +/-
TA6.NO, 1909 1909
ELEMENT VALUE VALUE
7MAY86
10:0?
1910
VALUE
< VALUE*LIHIT OF DETECTION DETERMINED DY INSTRUMENT SENST»SAMPLE DIL» AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIBITS
STDV
I/-
VALUE
NA
K
CA
H6
FE
HN
CO
MO
AL
AS
SE
CO
DE
CU
CR
NI
ZN
AG
TL
PB
LI
SR
V
DA
B
TI
153.
1590,
4970.
2730.
15000.
260.
4.9
<1.
17300.
<20.
13.
<.3
0.86
7.9
22.3
13.4
37.4
<1.
<5.
12.7
9.2
25,0
32.5
168.
6.9
338.
15.
150.
490.
270.
1500.
25.
1.0
1.4
1700.
20.
11.*
0,30
0,31
2,3
2,2
1,4
4.0
1,0
5.4
5.5
1.0
2.4
4,0
16.
1,5
33.
147.
1500.
4670.
2740.
15500.
316.
5.67
<1.
17100.
<20.
12.
<,2
0.89
7.8
21.7
13.1
36.5
<,9
<5.
14.2
9.01
24.8
32,2
180.
5.9
304.
14.
ISO.
460.
270.
1500.
31.
0.97
1.4
1700.
20.
12.
0.28
0.29
2.4
2.1
1.3
3.9
0.98
5.3
5.5
0.95
2.4
4.0
18.
1.4
30.
140.
1110.
4210.
2250.
13600.
387,
5.78
<1.
14600.
<17.
12,
<.2
0.73
8.0
22.3
18.1
36,7
<,9
<4.
15.3
7.66
22.2
27,8
153.
7.0
268.
14.
110.
420.
220.
1300.
38.
0.94
1.2
1400.
17,
10,
0,27
0,28
2.1
2.2
1.8
3.9
0.94
4.6
5.0
0.93
2.2
3.4
15.
1.3
26.
164.
1340.
7840.
2380.
13700.
217,
4.56
<1.
16100.
<19.
11.
<.2
0.77
6.4
21,5
12.1
32.5
<.B
<5.
14,5
7.71
43.3
30.3
153.
6.1
302.
16.
130.
780.
230.
1300.
• 21.
0.82
1,3
1600.
19.
10.
0.24
0,25
2.1
2.1
1.2
3.5
0.82
5.2
5.3
0.80
4.3
3.7
15.
1.3
30.
EPA/RSKERL/AOAfOK
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR!
LIST.LSTJ2261
PROJECT: OLD INGERS SITE T.A,
P184.8
CONCENTRATION IN: N6/K6 WET UT,
DATE
TIME
TAG.NO.
30DEC8S
14:01
56
1158
6ROUP2
STDV
f/-
56
PLOT 4
0-6* 10208
ELEMENT
NA
K
CA
H6
FE
MN
CO
NO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
VALUE
64.
1290.
4370.
2320.
13100.
257.
4.63
<1.
14800.
<17.
<10.
<.2
0.71
7.1
17.8
11.1
31.7
<.8
<4,
10.9
8.68
22.9
.29.4
163.
<1.
284.
VALUE
12.
130.
430.
230.
1300.
25.
0.84
1.2
1400.
17.
10.
0.24
0.26
2.0
1.7
1.1
3.4
0.84
4.4
4,9
0.88
2.2
3.6
16.
1.2
28.
30DEC85
14J02
4436
US?
6ROUP2PLOT5
0-6* 40209
STDV
4456
VALUE
59]
88?.
6590.
2110.
12000.
295.
5.73
1.2
11700.
0.59
6.6
15.7
10.2
30.6
12.2
6.91
26.6
24.2
155.
<1.
196.
VALUE
12.
90.
650.
210.
1200.
29.
0.81
1.0
1100.
14.
9.5
0.24
0.24
1.9
1.5
1.0
3.3
0.82
3.8
4.1
0.80
2.6
3.0
15.
1.0
19.
30DEC85 STBV
14.*04 */-
336 336
11S90UP 6RIND
6ROUP2PLOT5
0-6* 10209
VALUE
55.
1030.
3970.
2090.
12900.
302.
5.35
1.7
12900.
0.66
6.5
16.7
10.6
30.7
13.6
6.98
21.1
28.1
169.
<1.
223.
VALUE
300EC85 STDV
14:05 */-
4366 4366
11S9DUP 016 OF DUP 6RIND
6ROUP 2 PLOT 5
0-6f 10209
VALUE
11.
100.
390.
210.
1200.
29.
0.77
1.2
1200.
15.
10.
0.22
0.23
2.0
1.6
1.1
3.3
0.78
4.0
4.5
0.76
2.1
3.4
16.
1.0
22.
59,
1130.
4080.
2130.
13100.
280.
4.98
1.5
13600.
<16.
<10.
<,2
0.68
6.7
17.9
10.7
31.6
<.8
<4.
14.0
7.69
22.4
29.0
166.
<1.
249.
< VALUE«LINIT OF DETECTION DETERMINED BY INSTRUMENT SENSTtSANPLE DILt AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
VALUE
12.
110.
400.
210.
1300.
27.
0.83
1.2
1300.
16.
10.
0.24
0.25
2.0
1.7
1.1
3.4
0.83
4.2
4.7
0.81
2.2
3.5
16.
1.1
25.
EPA/RSKERL/AMiOK
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR:
LIST.LSTI2261
PROJECT: out INGERS SITE T.A,
P184.8
CONCENTRATION IN! KG/KG MET HT.
DATE
TIME
TA6.NO.
ELEMENT
NA
K
CA
H6
FE
HN
CO
HO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
30DEC85
13Z54
626
1155
CONTROL
W205
VALUE
76.
1280.
4980.
2430.
12900.
299.
5.17
<1.
14800.
<17.
<10.
<.2
0.70
6.5
17.0
11,1
39.0
<.8
<4.
9.5
8.49
22.4
28.7
152.
<1,
267.
STDV
*/-
626
PLOT 0-6'
VALUE
13.
130.
490.
240.
1200.
29.
0.83
1.1
1400.
17.
10.
0.24
0.25
2.0
1,6
1.1
4.1
0.83
4.4
4.7
0.86
2.2
3.5
13.
1.7
26.
STDV
2006
30DEC8S
13:56
2006
11S5DUP
CONTROL PLOT 0-6'DUP
M20S
VALUE
1250.
5120.
2410.
13100.
408.
5.75
<1.
14700.
0.71
6,6
16.4
11.0
33.6
10.1
8.40
23.1
29.4
163.
<1.
275.
VALUE
13.
120.
510.
240.
1300.
40.
0.84
1.1
1400.
17.
10.
0.24
0.26
2.0
1.6
1.1
3.6
0.84
4.4
4.7
0.85
2.3
3.6
16.
1.2
27.
30DEC85
13:58
4306
1156
GROUP
0-6* *02M
VALUE
769.
3760.
1960.
12300.
354.
5.02
<.9
11800.
0.60
6.2
15.3
9.8
28.3
9,9
5.65
18.2
22.0
154.
<.9
164.
< VALUE»LINIT OF DETECTION DETERMINED BY INSTRUMENT SENSTrSAHPLE DIL» AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
STDV
*/-
4306
NTROL PLOT2
6
VALUE
14.
78.
370.
190.
1200.
35.
0.78
0.97
1100.
14.
9.7
0.23
0.23
1.9
1.5
1,0
3.0
0.79
3.6
4.0
0.77
1.8
2.8
15.
0.99
16.
30DEC85
13:59
3056
1157
STDV
*/-
3056
6ROUP 1PLOT3
0-6' 10207
VALUE
101.
1390.
4100.
2200.
12500.
274.
4.80
<1.
14500.
<17.
<9,
<.2
0.71
6.6
16.5
10.6
28.7
<.8
<4.
9,6
8,57
22.1
28.9 .
148.
<1.
298.
VALUE
16.
130.
410.
220.
1200.
27.
0.81
1.1
1400.
17.
9,8
0,24
0.25
1.9
1.6
1.1
3.1
0.81
4.3
4.6
0.86
2.2
3.5
14.
1.2
29.
EPA/RSKERL/ADAiOK
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR:
LIST.LSTJ2257
PROJECT: INGERS SITE SAMPLES COLLECTEB 9/20/85
PI81,8
CONCENTRATION IN5 M6/K6 MET HT
DATE 1MOV8S STDV
TIME 14M2 */-
TA6.NO. 180R 180R
ELEMENT VALUE VALUE VALUE VALUE VALUE VALUE VALUE VALUE
MA
K
CA
N6
FE
m
CO
MO
AL
AS
SE
CD
BE
CU
CR
NI
»
AG
TL
PB
LI
SR
V
BA
B
TI
97,
1070.
4460.
2300.
13400.
444.
3,58
<1.
13800.
<17.
<10.
<.2
0.73
7.0
18,0
12.0
33.4
<,8
<4.
10.5
7.64
23.1
31,6
169.
3.1
281.
10.
100.
440.
230.
1300.
44.
0.83
1.1
1300,
17.
10.
0.24
0.25
2.1
1.7
1.1
3.6
0.83
4.4
4.5
0.81
2.3
3.8
16.
1.2
28.
< VALUE»LINIT OF DETECTION KTERHIND BY INSTRUMENT SENSTiSANPLE OILi AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SNNIFICANT BI6ITS
EPA/RSKERL/AOAfOK
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR:
LIST.LSTJ2257
PROJECT: INGERS SITE SAMPLES COLLECTED 9/20/93
P181.8
CONCENTRATION IN! H6/K6 UET HT
DATE
TIME
TAB.NO.
ELEMENT
m
K
CA
N6
FE
MN
CO
MO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
* •••• *^_*
1NOV8S
14:05
186
PLOT 3
F1609
VALUE
103.
1110.
37W.
2210.
12200.
244.
4.05
<1.
13500.
<16.
AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
EPA/RSXERL/ADAiOK
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR: .
LIST.LSTJ2257
PROJECT: INGERS SITE SAMPLES COLLECTED 9/20/95
P181.8
CONCENTRATION IN! H6/K6 MET VT
1NOVB5
14:02
1MB
GROUP 2 PLOT
OHO
VALUE
93.
897.
4040.
1910.
11300.
260.
4.22
5.8
11600.
0.41
7,5
18.1
10.8
38.3
15.?
6.74
23.2
25.0
192.
2.55
177.
< VALUE*LIHIT OF DETECTION DETERMINE) BY INSTRUMENT SENSTiSANPLE DILi AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
DATE
TINE
TA6.NO.
ELEMENT
NA
K
CA
H6
FE
MN
CO
HO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
AG
TL
PB
LI
SR
V
BA
B
TI
1NOV85
13:59
183
GROUP 2
0107
VALUE
94.
1010.
3700.
2030.
12100.
197.
3.95
2.2
13000.
<16.
<9.
<.2
0,60
6.6
17,5
10.1
42,2
<,7
<4,
12.6
7.19
20.9
28.7
156.
5.4
251.
STDV
*/-
183
PLOT 4 0-6'
VALUE
10.
100.
370.
200.
1200.
19.
0.79
1.2
1300.
16.
9.5
0,23
0,24
1,9
1.7
1.0
4.5
0.79
4,1
4.4
0,77
2.0
3.4
IS.
1.1
25.
1NOV85
14:
184
GROUP2
0110
VALUE
100.
1030.
3380.
1980.
11500.
181.
3.85
5.3
12600.
<15.
<9.
<,2
0,63
7.5
19.0
10,0
32.7
<.7
<3.
14.5
7.46
20.3
26.4
180.
4.5
233.
. &u ••Mk««UMM
STDV
t/-
184
PLOT 5 0-6'
VALUE
11.
100.
330.
190.
1100.
17.
0.78
1.5
1200.
IS.
9.1
0.23
0.23
1.8
1.8
1.0
3.5
0,78
3.9
4.5
0.76
2.0
3,2
18.
1.1
23.
•»•• 4^VUM9 M A MflM
STDV
*/-
1B4D
.OT 5 0-6'
DIGESnON
VALUE
10.
90.
400.
190.
1100.
25.
0,75
1.4
1100.
14.
8.9
0.22
0.22
1.7
1.7
1.0
4.1
0.76
3.6
4.3
0.74
2.3
3.0
19.
0.98
17.
1NOV85
14!03
185
PLOT 2 0-61
F1608
VALUE
102.
951.
3750.
2070.
12300.
229.
4.13
<1.
13100.
<16.
<9.
<.2
0.65
6,2
16.3
10.2
29,8
<,7
<4,
12,1
7.04
20.2
27.8
138.
3.0
261.
STDV
+/-
185
VALUE
11.
96.
370*
200.
1200.
22.
0.73
1.1
1300.
16.
9.6
0.21
0.22
1.9
1.6
1,0
3.2
0,73
4.0
4.4
0.71
2,0
3.3
13.
1.1
26.
EPA/RSXERL/ADA»OK
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR*
LIST.ISTI2257
PROJECT: INSERS SITE SAMPLES COLLECTED 9/20/85
P181.8
CONCENTRAnON IN: MS/KB VET HT
BATE 1NOV83 STDV 1NOV85 STDV 1NOV8S STDV 1NOV85 STDV
TINE 13544 f/- 13:45 */- 13J47 */- 13J49 */-
TA6.NO. 180 180 180B 180D 181 181 182 182
PLOT 1 CONTROL 0-6' PLOT 1 CONTROL 0-6' GROUP 1 PLOT 2 0-6' GROUP 1 PLOT 3 0-4*
F1607 F1607 0101 0104
DUPLICATE DIGESTION
ELEMENT VALUE VALUE VALUE VALUE VALUE VALUE VALUE VALUE
NA
K
CA
N6
FE
MN
CO
NO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
TL
PB
LI
SR
V
BA
B
TI
108.
1180.
4390.
2310.
13400.
437.
S.63
<1.
14000.
<17.
<10.
<,2
0.78
7.1
18.1
12.0
33.0
<.8
<4.
10.4
8.17
23.1
30.9
170.
3.3
275.
11.
110.
430.
230.
1300.
43.
0.83
1.1
1400.
17.
10.
0.24
0.25
2.1
1.7
1.2
3.6
0.83
4.3
4.6
0.82
2.3
3.7
17.
1.2
27.
101.
1040.
3780.
2190.
13400.
327.
4.41
<1.
13400,
<16.
<10.
<.2
0.70
6,6
16,9
11.8
32.4
<.8
<4,
12.2
7.56
20.3
29.3
156.
6.7
265.
11.
100.
370.
220.
1300.
32.
0.82
1.1
1300.
16.
10.
0.24
0.25
2.1
1.6
1.1
3.5
0.82
4.3
4.6
0.80
2.0
3.5
IS.
1.2
26.
154.
1140.
3440.
2060.
12500.
298.
5.06
<1.
13800.
<17.
<9.
<,2
0.71
6.4
17.0
11.2
30.7
<.7
<4.
14.0
7.54
20.6
29.5
156.
5.0
300.
16.
110.
340.
200.
1200.
29.
0.79
1.1
1300.
17.
9.8
0.23
0.24
1,9
1.6
1.1
3.3
0.79
4.2
4.7
0.78
2.0
3.5
15.
1.2
30.
149,
954.
6260.
1980.
11800.
231.
4.14
<1.
12600.
<15.
<9.
<.2
0.64
6.6
16.4
9.89
29.5
<,8
<4.
10.2
6.95
26.5
25.9
135.
3.4
245.
15.
96.
620.
190.
1100.
22.
0.80
1.0
1200.
15.
9,3
0.23
0.24
1.8
1.6
0.98
3.2
0.80
4.1
4.1
0.79
2.6
3.1
13.
1.1
24.
< VALUE*LINIT OF DETECTION DETERMINED BY INSTRUMENT SENSTiSANPLE BILt AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
EPA/RSKERL/ADA»OK
-------
ELEMENTAL CONSTITUENTS ANALYSIS BY ICAP
FOR:
LIST.LSTI2263
PROJECT: IMSERS SITE SAMPLES-SUPERFUNB
P174.8
CONCENTRATION IN: MS/KB BET BEI6HT EXCEPT FOR 1550(SLUWE + LIQUID) IN N6/L
DATE STDV STDV STDV STDV STDV STDV STDV ' STDV
TIME 14: V- 14U8 +/- 14:19 +/- 14:21 */-
TA6.IKJ. 308H 308H 4306 4306 516 516 4106 4106
1550 1550 DUPLICATE 1550 (LIQUID FROM SLUB6E)t$ 1546 REBURN
SLUD6E SLUB6E -RESULTS IN N8/L-
ELEKENT VALUE VALUE VALUE VALUE VALUE VALUE VALUE VALUE
MA
K
CA
H6
FE
MN
CO
MO
AL
AS
SE
CD
BE
CU
CR
Ml
IN
A6
TL
PB
LI
SR
V
BA
B
TI
126.
40.
227,
96,
1550,
12.0
3.6
28.9
541,
<3.
<3.
<«3
<.3
19.2
73.7
8.0
89.4
<1»
3.4
123.
<1.
38.8
11.}
886.
3.9
<11.
13.
29.
24.
11.
150.
1.1
1.1
2.9
54.
3.5
3.8
0.35
0.35
1.9
7.4
1.1
9.1
1.1
2.3
12.
1.1
3.8
1.2
88.
1.1
11.
118.
35.
232.
93.
1550.
12.1
3.4
27.2
497.
<3.
<3«
<»3
<«3
18.1
72.6
8.2
86.5
<1.
<2.
122.
<1.
37.3
11.4
844.
<1.
<10.
12.
27.
25.
10.
150.
1.1
1.0
2.7
SO.
3.3
3.5
0.32
0.32
1.8
7.2
1.0
8.8
1.0
2.2
12.
1.0
3.7
1.2
84.
1.1
10.
128.
5.27
65.3
35.9
233.
3.09
0.22
0.20
13.8
<•!
<»2
<»01
<.009
0.08
1.53
0.39
1.90
<.03
<.06
0.38
0.05
0.82
0.05
0.21
0.44
<»3
12.
0.82
6.5
3.6
23.
0.30
0.03
0.03
1.4
0.10
0.22
0.01
0.009
0.05
0.15
0.04
0.19
0.03
0.06
0,06
0.03
0.08
0.03
0.03
0.04
0.33
177.
3160.
4390.
4610.
19200.
246.
5.88
<1.
27600.
<25.
<17.
<»1
1.45
18.4
30.0
16.0
65.7
<.6
<7.
15.8
17.2
36.0
58.8
141.
20.3
223.
18.
310.
440.
460.
1900,
24.
0.66
1.9
2700.
25.
17.
0,19
0.20
3.7
2.9
1.6
6.9
0.64
7.0
5.8
1.7
3.6
6.7
14.
5.1
22.
< VALUE«LIMIT OF DETECTION DETERMINED BY INSTRUMENT SENSTiSAMPLE DIL. AND MATRIX INTERFERENCE.
RESULTS ACCURATE TO 2 SIGNIFICANT DIGITS
EPA/RSKERL/ADAfOK
» SAMPLE COMPOSED OF TUO PHASES (SLUDGE AND LIQUID). LIQUID MAS TAKEN FROM SURFACE OF SAMPLE AND ANALYZED SINCE
THE SAMPLE COULD NOT BE SAMPLED REPRESENTATIVELY. RESULTS REPORTED ABOVE AS N6/L OF LIQUID.
-------
ELEMENTAL CONSTITUENTS ANALYSIS IY ICAP
FOR:
LJST.LSTJ2263
PROJECT: INGERS SITE SAMPLES-SUPERFUND
P174.8
CONCENTRATION IN: M6/K6 VET HEIGHT EXCEPT FOR 1SSO(SIUD6E + LIQUID) IN M6/L
STDV STDV
13:55 +/-
426 428
1549 DUPLICATE
BURIED HASTE
DATE
TINE
TA6.NO,
ELEMENT
NA
K
CA
H6
FE
HN
CO
MO
AL
AS
SE
CD
BE
CU
CR
NI
ZN
A6
Tl
PB
LI
SR
V
BA
B
' TI
STDV
13:53
4026
154?
BURIED
VALUE
487.
<50.
183.
69.
457.
5.3
<2,
<2.
189.
7,7
<6,
<,6
<.6
10.9
20.5
8.4
58.8
<2.
5.4
27.5
<2.
9.0
28.1
92.4
7,5
<20.
STDV
*/-
4026
HASTE
VALUE
50,
50.
21.
20.
46.
2.0
2.0
2.0
20.
6.0
6.0
0.60
0.60
2.0
2.1
2.0
6.1
2.0
4.0
4.0
2.0
2.0
2.8
9.2
2,0
20.
VALUE
543,
<46.
194.
73.
472.
5.2
216.
7.5
12.1
19,8
9.2
67.4
-------
MI
il
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5 3
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INITIAL
INITIAL
oCN
-------
SHEET NO.
CHAIN OF CUSTODY RECORD
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Mnjrrr MAMr^ Ji
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DATE /Til
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BEGAN
1510
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^1525
^
~JLA/X.y (J r V
INITIAL
^
^
ttf
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COMMENTS
////V//7^f
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> ncrr/TMF
nATT/TIMF
F MIT/TIMT
BATT/TIMr
-------
SHEET NO..
PROJECT NAME
PROJECT no
CHAIN OF CUSTODY RECORD
SAMPLER (S) SIGNATUR
-------
-------
-------
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5
i
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SHEET NO _ie±l OF
PROJECT NAMC
PROJECT NO.
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SAMPLER (SI SIGNATURE
^
SAMPLE
IDENTIFICATION
SAMPLING
LOCATION
DATE
SAMPLED
SAMPLE TYPE
10 AM OIL
VOLUME
TO BE
COLLECTED
NO OF
CONTAINERS
TIME
COLLECTIO
BEGAN
TIME
COLLECTION
COMPLETED
COMMENTS
LpL
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X
X
y ig"
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lt>fyH]-x fV'-<
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RELINQUISHED BY
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, DATE/TIME
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!^££Jfl>VE(xivED BY NAME"
_ RECEIVED BY: NAME .
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DATE /TIME.
DATE/TIME
HATF/TIMF
-------
PROJECT
PROJECT NO.
CHAIN OF CUSTODY RECORD
SAMPLER(S) SIGNATUR
SHEET NO
b/V/S1^ £ VvS/ffK
SAMPLE
IDENTIFICATION
SAMPLING
LOCATION
DATE
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10 AW OIL
VOLUME
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NttOF
CONTAINERS
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BEGAN
TIME
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COMMENTS
£>*vz/ay/
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/
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RELINQUISHED BY: NAME
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AUTHORIZATION FOR DISPOSAL.
.DATE/TIME
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DATE/TIME
7/z3
BY^ NAME.
RECEIVED BY: NAME.
RECEIVED BY: NAME .
DISPOSED BY:
DATE/TIME-
DATE/TIME .
DATE/TIME.
DATE/TMC.
-------
EEJ
PROJECT NAME
PROJECT M
SHEET NO..
.OF
CHAIN OF CUSTODY RECORD
SAMPLER (S) SIGNATUR
SAMPLE
IDENTIFICATION
SAMPLING
LOCATION
DATE
SAMPLED
SAMPLE TYPE
10 AIM OIL
VOLUME
TO BE
COLLECTED
NO. OF
CONTAINERS
TIME
COLLECTION
BEGAN
TIME
COLLECTION
COMPLETED
COMMENTS
f /
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12^1
(37,4
L
frrrM*+Ml A"-6>"
if
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t
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it
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RELINQUISHED BY n.*^^<^T)(JL^{ '^pffiff^K* /Tim^/Cyj^ lOOJei^r.^^
RELINQUISHED BY: NAME DATE/TIME RECEIVED
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RELINQUISHED BY: NAME
AUTHORIZATION FOR DISPOSAL.
. DATE/TIME
DATE/TIME .
RECEIVED
DISPOSED
BY NAME.
BY: NAME.
BY: NAME .
BY:
DATE/TIME-
DATE/TIME .
DATE/TIME .
DATE/TIME.
-------
«Mrrr no C—^ or C •
PROJECT NAME.
PROJECT NO
Lna-fS''
CHAIN OF CUSTODY RECORD
SAMPLER(S) SIGNATURE
SAMPLE
IDENTIFICATION
SAMPLING
LOCATION
DATE
SAMPLED
SAMPLE TYPE
io *m OIL
VOLUME
TO BE
COLLECTED
NO. OF
CONTAINERS
TIME
COLLECTION
BEGAN
TIME
COLLECTION
COMPLETED
COMMENTS
''&>
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ti'-lZ
!*>.>
nf'-ff'
ti
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.DATE/TIME
. DATE/TIME
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RECEIVED BY: NAME.
RECEIVED BY: NAME.
RECEIVED BY: NAME
DISPOSED BY!
DATE/TIME.
DATE/TIME.
DATE/TIME.
DATE/TIME.
------- |