EPA/540/2-89/034
SUPERFUND TREATABILITY
CLEARINGHOUSE
Document Reference:
ECOVA Corporation. "Final Report: Soil Treatment Pilot Study Brio/DOP Site."
Technical Report No. 861014/1 (Ecova No.) prepared for U.S EPA Brio Site Task Force.
Approximately 130pp. June 1987.
EPA LIBRARY NUMBER:
Superfund Treatability Clearinghouse - EZZA
-------
SUPERFUND TREATABILITY CLEARINGHOUSE ABSTRACT
Treatment Process: Biological - Aerobic
Media: Soil/Generic
Document Reference: ECOVA Corporation. "Final Report: Soil Treatment
Pilot Study Brio/DOP Site." Technical Report No.
861014/1 (Ecova No.) prepared for U.S EPA Brio Site
Task Force. Approximately 130 pp. June 1987.
Document Type: Contractor/Vendor Treatability Study
Contact: Louis Barinka
U.S. EPA - Region VI
1445 Ross Avenue
12th Floor, Suite 1200
Dallas, TX 75202
212-655-6735
Site Name: Brio DOP Site (NPL)
Location of Test: Friendswood, TX
BACKGROUND; Bench and pilot-scale studies were conducted to demonstrate the
feasibility of using solid-phase biodegradation for destroying portions of
organic constituents present in the soil. The predominant constituents at
the BRIO DOP site located in Texas were volatile compounds such as: methylene
chloride, 15-17,000 ppb; 1,2-dichloroethane, 25-195,000 ppb; 1,1,2-
trichloroethane, 25-195,000 ppb. Semivolatile compounds were present in
lower concentrations: phenanthrene, 1,392-15,083 ppb; anthracene and
fluorene, 440-563 ppb (single samples only).
OPERATIONAL INFORMATION; Aerobic microorganisms present in soil samples
removed from the site ranged from 10 to 10 colony forming units per gram
weight of wet soil, indicating the site contained a diverse microbial popula-
tion. Bench-scale and pilot-scale tests were conducted. The pilot-scale
solid phase treatment facility consisted of a lined soil treatment area with
a leachate collection system, water/nutrient distribution system, emission
control system, a microbiological management system, and greenhouse enclosure
and support facilities. The pilot facility was operated for 94 days commenc-
ing in January of 1987. Two hundred (200) cubic yards of soil removed from
the site were placed in the pilot facility, inoculated with microorganisms,
nutrients were added (inorganic N&P), and the soils were tilled daily to
ensure contact and aeration. Tilling also facilitated air stripping of the
more volatile organics. Volatile compounds were trapped by activated carbon
absorbers at the pilot facility.
PERFORMANCE; The pilot-scale treatment facility demonstrated under field
conditions that a solid-phase treatment process could be used to successfully
treat the organic constituents present in the site soil. The process removed
the volatile organic compounds by air stripping, and destroyed semivolatile
organic compounds by biodegradation. More than 99£ of the volatile organic
compounds were removed within the first 21 days of operation. However, the
3/89-20 Document Number: E2ZA
NOTE: Quality assurance of data may not be appropriate for all uses.
-------
biodegradation of the semivolatile organic constituents was much slower. It
was estimated that approximately 131 days would be required to reduce the
phenanthrene concentrations to non-detectable levels in the treatment
facility. The time required to treat affected soils and materials (volatile/
semivolatile organics) in a solid phase treatment process might be
unacceptably long if rapid remediation is required.
No actual tests were conducted on a full scale facility. However, the
authors discuss the feasibility of full scale tests and postulate that
aqueous phase biodegradation could enhance the rate of removal of the organic
components by improving the contact between microorganisms, nutrients, and
oxygen. No treatment cost data was provided. Numerous references to the
biodegradation of specific organic compounds are contained in this document.
EPA analytical methods were utilized to analyze for volatile organics. A
QA/QC plan is contained in the document along with a statistical analysis of
the data.
CONTAMINANTS;
Analytical data is provided in the treatability study report.
breakdown of the contaminants by treatability group is:
The
Treatability Group
WOl-Halogenated Aromatic
Compounds
W04-Halogenated Aliphatic
Solvents
W07-Heterocyclics and
Simple Aromatics
W08-Polynuclear Aromatics
W09-0ther Polar Organic
Compounds
CAS Number
108-90-7
79-34-5
79-00-5
75-09-2
75-34-3
100-41-4
100-42-5
71-43-2
108-88-3
1330-20-7
91-20-3
85-01-8
91-57-6
67-64-1
78-93-3
Contaminants
Chlorobenzene
1,1,2,2,-Tetrachloroethane
1,1,2,-Trichloroe thane
Methylene Chloride
1,1-Dichloroethane
Ethylbenzene
Styrene
Benzene
Toluene
Xylenes
Naphthalene
Phenanthrene
2-Methylnaphthalene
Acetone
2-Butanone
3/89-20 Document Number: EZZA
NOTE: Quality assurance of data may not be appropriate for all uses.
-------
FINAL REPORT
SOIL TREATMENT PILOT STUDY
BRIO DOP SITE
FRIENDSWOOD, TEXAS
SUBMITTED TO
BRIO SITE TASK FORCE
JUNE 1987
-«**§
*«**
-«**
THEON-SITE
HAZARDOUS WASTE
MANAGEMENT COMPANY
E C 0 V A
-------
FINAL REPORT
SOIL TREATMENT PILOT STUDY
BRIO/DOP SITE
Friendswood, Texas
Prepared by:
ECOVA CORPORATION
15555 N.E. 33rd
Redmond, Washington 98052
June 1987
Project Number 861014
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SUMMARY AND CONCLUSIONS
A preliminary assessment during the Remedial Investigation indicated that solid-phase
biodegradation might be a suitable technology for destroying a portion of the organic
constituents present in the soil at the Brio/DOP site. Pit O was selected as the pit best
suited to demonstrate this technology.
Ecova Corporation conducted an evaluation of the amenability of the organic constituents
in Pit O to solid-phase biodegradation. The evaluation:
o Defined the types of organic constituents present in Pit O soil, and assessed the
amenability of these compounds to biodegradation.
o Defined the level of existing microbiological activity within Pit O.
o Demonstrated at the bench-scale level that solid-phase biodegradation processes can
be used for removing or destroying the organic constituents present in Pit O soil.
o Demonstrated on-site at the pilot-scale level that solid-phase biodegradation
processes can be used for removing or destroying the organic compounds present in
Pit O soil.
o Evaluated potential full-scale systems capable of removing or destroying organic
compounds in on-site soil and affected material.
Pit O Characterization
The initial step in the solid-phase biodegradation evaluation was to determine the types
and concentrations of organic constituents present in Pit O soil, so that their amenability
to biodegradation could be assessed. In addition, the number of aerobic microorganisms
present in samples was determined to define the level of existing microbiological activity
within Pit O.
The chemical analyses demonstrated considerable variation in the types and concentra-
tions of organic constituents present in Pit O. The predominant constituents were
volatile compounds such as methylene chloride, 1,2-dichloroethane and 1,1,2-trichloro-
ethane. These compounds were detected at concentrations ranging from 15 to 17,000 ppb,
25 to 195,000 ppb, and 25 to 195,000 ppb respectively. Semi-volatile compounds such as
phenanthrene, anthracene, and fluorene were also present, but generally at lower
concentrations. Phenanthrene concentrations ranged from 1,392 to 15,083 ppb, while
anthracene and fluorene were detected in only single samples with concentrations of 440
ppb and 563 ppb, respectively. Constituent concentrations generally decreased with
depth.
The organic compounds detected in Pit O can be grouped into the following categories:
o Ketones (e.g., acetone and 2-butanone)
o Short chain chlorinated hydrocarbons (e.g., chloroethanes)
o Chlorinated aromatic hydrocarbons (e.g., chlorobenzenes)
o Aromatic hydrocarbons (e.g., phenanthrene)
861014/1-FINAL REPORT ii
-------
The numbers of aerobic microorganisms present in the samples removed from Pit O
ranged from 10* to 10* colony forming units (CPU) per gram wet weight of soil. Higher
numbers of microorganisms were present at the surface than the subsurface, which is
typical since more oxygen is available to microorganisms at the soil/air interface. All of
the samples contained several different types of microorganisms differentiated on the
basis of colony morphology on the surface of agar plates. This is typical for environ-
mental samples. In summary, these data demonstrated that Pit O contained a diverse
microbiai population.
Feasibility of On-Site Biodegradation
A literature review demonstrated that all of the organic constituents detected in Pit O
were amenable to solid-phase biodegradation. In order to substantiate this conclusion, a
bench-scale evaluation was performed to demonstrate the biodegradability of the organic
compounds found in Pit O.
Bench-Scale Treatabilitv Evaluation
The bench-scale treatability evaluation demonstrated that the semi-volatile organic
constituents present in Pit O soil were amenable to biodegradation, and that biodegrada-
tion would be the mechanism by which these compounds would be destroyed. The
evaluation also demonstrated that while the volatile compounds are biodegradable, because
of their physical properties, volatilization would be the mechanism by which these
constituents would be removed in the proposed treatment facility.
Since effective removal of volatile organic compounds and biodegradation of semi-volatile
compounds was demonstrated by this bench-scale testing, Ecova recommended the
construction and operation of a pilot-scale, solid-phase biodegradation facility in order to
demonstrate the effectiveness of this process under field conditions.
Pilot-Scale Treatment Facility
The pilot-scale treatment facility demonstrated under field conditions that a solid-phase
treatment process could be used to successfully treat the organic constituents present in
Pit O soil. The process removed the volatile organic compounds by air stripping, and
destroyed semi-volatile organic compounds by biodegradation. More than 99% of the
volatile organic compounds were removed within the first 21 days of operation of the
pilot test. However, the biodegradation of the semi-volatile organic constituents was
much slower. For example, it was estimated that approximately 131 days would be
required to reduce the phenanthrene concentration to non-detectable levels in the
treatment facility.
Full-Scale Treatment Facility
The pilot-scale treatment facility effectively demonstrated an efficient, cost-effective
process for remediating the organic compounds found in Pit O soil. The process removed
volatile organic compounds by air stripping and destroyed semi-volatile organic compounds
by biodegradation. Although such a facility would be effective in reducing the con-
centrations of volatile and semi-volatile organic compounds, the time required to treat
affected materials and soils might be unacceptably long. An aqueous biodegradation
process would increase the rate of removal of organic compounds. Ecova Corporation
861014/1-FINAL REPORT iii
-------
therefore concludes that an aqueous phase biodegradation process is the optimum system
for removing the organic compounds in the required period of time, assuming that site
remediation must be accomplished in less than five years.
Conclusions
o A solid-phase treatment process can be used for removing or destroying the organic
compounds detected in Pit O soil.
o The process removes volatile organic compounds by air stripping, and destroys semi-
volatile organic compounds by biodegradation.
o Although such a facility would be effective in reducing the concentrations of
volatile and semi-volatile organic compounds, the time required to treat affected
materials and soils by a solid-phase treatment process might be unacceptably long.
o An aqueous phase biodegradation process would increase the rate of removal of
organic compounds. Ecova Corporation therefore concludes that an aqueous phase
biodegradation process is the optimum system for removing the organic compounds
in the required period of time, assuming that site remediation must be accomplished
in less than five years.
861014/1-FINAL REPORT iv
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TABLE OF CONTENTS
SUMMARY AND CONCLUSIONS
Pit O Characterization ii
Feasibility of On-Site Biodegradation ijj
Bench-Scale Treatability Evaluation in
Pilot-Scale Treatment Facility iii
Full-Scale Treatment Facility iii
Conclusions iv
1. INTRODUCTION 1-1
2. PIT O CHARACTERIZATION
2.1 Introduction 2-1
2.2 Sample Collection 2-1
2.3 Chemical Evaluation 2-1
2.4 Microbiological Evaluation 2-1
3. FEASIBILITY OF ON-SITE BIODEGRADATION
3.1 Introduction 3-1
3.2 Degradation Processes 3-1
3.3 Microbial Degradation 3-3
3.4 Half-Life Values 3-4
3.5 Conclusion . 3-6
4. BENCH-SCALE TREATABILITY EVALUATION
4.1 Introduction 4-1
4.2 Pit O Microbial Characterization 4-1
4.3 Bench-Scale Microcosm Evaluation 4-1
4.4 m Summary and Conclusions 4-6
5. ON-SITE PILOT SCALE TREATMENT FACILITY
5.1 Introduction 5-1
5.2 Treatment Facility Construction and Operation 5-1
5.3 Initial Treatment Facility Conditions 5-2
5.4 System Performance 5-14
5.5 Summary and Conclusions 5-18
6. FULL-SCALE TREATMENT SYSTEM
6.1 Feasibility of Aqueous Phase Biodegradation 6-1
6.2 Full-Scale Aqueous-Phase Biodegradation Facility 6-1
6.3 Summary and Conclusions 6-4
7. CONCLUSIONS 7-1
8. REFERENCES CITED 8-1
861014/1-FINAL REPORT v
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TABLE OF CONTENTS
(Continued)
APPENDICES
A Glossary
B Analytical Chemistry Methods
C Analytical Chemistry Data: Pit O Characterization
D Analytical Chemistry Data: Bench-Scale Biodegradation Evaluation
E Facility Construction and Operation
F Analytical Chemistry Data: Pilot-Scale Evaluation of Solid-Phase
Biodegradation
G Analytical Chemistry Data: Pilot-Scale Evaluation of Aqueous
Biodegradation
H Statistical Analysis of Data
I Laboratory Quality Assurance/Quality Control Data
J Microbiological Methods
K Microbiology Data
L Operational Sampling Locations
861014/1-FINAL REPORT vi
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TABLES
Page
2-1 Pit O Organic Constituent Analysis Baseline Characterization 2-3
2-2 Pit O Microbial Evaluation: Enumeration of Aerobic Heterotrophic 2-4
Microorganisms
3-1 Reported Half-Life Values for Compounds in Soil Treatment 3-6
4-1 Volatile Organic Carbon Compound Concentrations in Enrichment 4-4
Culture CH0132
4-2 Semi-Volatile Organic Carbon Compound Concentrations in Enrichment 4-5
Culture CHOI 32
5-1 Removal of Volatile Organic Compounds in the Control Lane 5-3
5-2 Removal of Volatile Organic Compounds in the Nutrient-Adjusted Lane 5-4
5-3 Removal of Volatile Organic Compounds in the Single-Inoculated Lane 5-5
5-4 Removal of Volatile Organic Compounds in the Multiple-Inoculated Lane 5-6
5-5 Degradation of Semi-Volatile Organic Compounds in the Control Lane 5-7
5-6 Degradation of Semi-Volatile Organic Compounds in the Nutrient-Adjusted 5-8
Lane
5-7 Degradation of Semi-Volatile Organic Compounds in the Single-Inoculated 5-9
Lane
5-8 Degradation of Semi-Volatile Organic Compounds in the Multiple-Inoculated 5-10
Lane
5-9 Initial Microorganism Concentrations 5-11
5-10 Initial Percent Mineralization of 14C-Glucose to 14C-CO2 5-12
5-11 Initial Percent Mineralization of 14C-Phenanthrene to 14C-CO2 5-13
5-12 Numbers of Aerobic Heterotrophic Microorganisms in Pilot-Test Treatment 5-15
Lanes
5-13 Percent Mineralization of 14C-Glucose to 14C-CO2 5-16
5-14 Percent Mineralization of 14C-Phenanthrene to 14C-CO2 5-17
861014/1-FINAL REPORT vii
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TABLES
(Continued)
Page
5-15 Phenanthrene Half-Life Values in Treatment Lanes 5-19
5-16 Calculation of Phenanthrene Half-Life Value 5-20
5-17 Effect of Initial Concentration on Phenanthrene Degradation 5-21
6-1 Half-Life Values for Phenanthrene in the Fermentation Vessel 6-2
6-2 Half-Life Values for Polynuclear Aromatic Hydrocarbons in Bench-Scale Aqueous 6-2
Phase Systems
861014/1-FINAL REPORT viii
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FIGURES
Page
2-1 Pit O Sampling Locations 2-2
3-1 Typical Degradation Kinetics 3.5
4-1 Pit O Microbial Evaluation: Typical Growth Patterns in Enrichment Cultures 4-3
5-1 Reduction in Phenanthrene Concentration in Pilot-Test Area 5-22
6-1 Process and Instrumentation Diagram, Aqueous-Phase System 6-3
861014/1-FINAL REPORT ix
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Chapter 1
INTRODUCTION
-------
1. INTRODUCTION
A preliminary assessment during the Remedial Investigation of the Brio/DOP site indi-
cated that solid-phase biodegradation might be a suitable technology for destroying a
portion of the organic constituents present in the soil at the site. Pit O was selected as
the pit best suited to demonstrate this technology.
Ecova Corporation was retained to perform a pilot study and evaluation of the biodegrad-
ability of the organic compounds present in Pit O soil. The objectives of the project
were to:
1. Review existing information on the solid-phase biodegradation of the organic
compounds present in Pit O soil. The object of the review was to assess the
potential for using solid-phase biodegradation to destroy the organic constituents
present in the soil.
2. Demonstrate at the bench-scale level that solid-phase biodegradation could be used
to destroy the organic constituents present in the soil.
3. Perform an on-site, pilot-scale demonstration of solid-phase biodegradation.
4. Evaluate potential full-scale systems capable of removing or destroying organic
compounds in on-site soil and affected material.
Major project tasks included the collection of initial soil samples, microbiological and
chemical analyses of these samples, treatment facility design and construction, pilot
system startup and operations, and chemical monitoring support during operations. Work
was accomplished on-site and in the Ecova corporate laboratories in Redmond, Washing-
ton.
Laboratory activities included the following:
o Microbial and chemical baseline analysis of Pit O soils
o Monitoring of nutrient concentrations during the pilot-scale test
o Monitoring of microbial populations during the bench-scale and pilot-scale tests
o Radiotracer analyses to assess biodegradation potential during the pilot-scale test
Field activities included the following:
o Initial sampling of Pit O and site characterization
o Design and construction of the pilot-scale treatment facilities
o Production of inocula (biodegrading microorganisms)
o Removal of soil from Pit O and placement in the treatment facility
o Operation of pilot-scale treatment facility
o Monitoring of air, temperature, humidity, and moisture content
o Maintenance of pilot study facilities
o Control of operational parameters such as aeration, moisture content, and particle
size of soil
o Sample collection for laboratory analysis
861014/1-FINAL REPORT 1-1
-------
Following final sample collection on April 30, 1987, the treatment facility was closed and
the mobile support units shut down.
This report describes and presents the results of the project activities described above.
The chapters are organized around the five main project activities: Pit O characteriza-
tion, literature review, bench-scale test, pilot-scale demonstration, and full-scale treat-
ment evaluation.
The contents of Pit O are characterized in Chapter 2 in terms of microbial activity and
the type and concentration of organic compounds present in the pit backfill. Chapter 3
reviews the existing information on the biodegradability of the organic compounds found
in Pit O, while Chapter 4 proves their biodegradability through bench-scale testing.
Another treatment method evaluated on a bench-scale basis in Chapter 4 is air stripping.
Chapter 5 evaluates the results of pilot-scale treatment facility operation and Chapter 6
discusses potential full-scale on-site treatment systems suitable for treating the organic
compounds found at the Brio/DOP site.
861014/1-FINAL REPORT 1-2
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Chapter 2
PITO CHARACTERIZATION
-------
2. PIT O CHARACTERIZATION
2.1 Introduction
The initial step in the solid-phase biodegradation evaluation was to determine the
types and concentrations of organic constituents present in Pit O soil, so that their
amenability to biodegradation could be assessed. In addition, the number of aerobic
microorganisms present in samples was determined to define the level of existing
microbiological activity within Pit O.
2.2 Sample Collection
Nine soil samples from three locations (Figure 2-1) were removed by hand auger
from Pit O on December 2 and 3, 1986. At each location, composite samples were
taken of depths of 0 to 5 ft, 5 to 10 ft, and 10 to 15 ft. A 0 to 2 ft field
background sample was removed from property at the corner of Beamer Road and
Dixie Farm Road. The samples were delivered to Ecova's Redmond, Washington,
laboratory for chemical and microbiological analysis.
2.3 Chemical Evaluation
The Pit O samples and background soil sample were analyzed for volatile and semi-
volatile organic constituents. The chemical analyses demonstrated considerable
variation in the types and concentrations of organic constituents present in Pit O
(Table 2-1). The predominant constituents were volatile compounds such as
methylene chloride, 1,2-dichloroethane and 1,1,2-trichloroethane. These compounds
were detected at concentrations ranging from IS to 17,000 ppb, 25 to 195,000 ppb,
and 10 to 1,600,000 ppb, respectively. Semi-volatile compounds such as phenan-
threne, anthracene, and fluorene were also present, but generally at lower con-
centrations. Phenanthrenc concentrations ranged from 1,392 to 15,083 ppb, while
anthracene and fluorene were detected in only single samples with concentrations of
440 ppb and 563 ppb, respectively. Constituent concentrations generally decreased
with depth.
The organic compounds detected in Pit O (Table 2-1) can be grouped into the
following categories:
o Ketones (e.g., acetone and 2-butanone)
o Short chain chlorinated hydrocarbons (e.g., chloroethanes)
o Chlorinated aromatic hydrocarbons (e.g., chlorobenzenes)
o Aromatic hydrocarbons (e.g., phenanthrene)
2.4 Microbiological Evaluation
The numbers of aerobic microorganisms present in the samples removed from Pit O
ranged from 10^ to 105 colony forming units (CFU) per gram wet weight of soil
(Table 2-2). Higher numbers of microorganisms were present at the surface than
the subsurface, which is typical since more oxygen is available to microorganisms at
the soil/air interface. The field background sample contained 10" CFU per gram
wet weight of soil. All of the samples contained several different types of micro-
organisms differentiated on the basis of colony morphology on the surface of agar
plates. This is typical for environmental samples. In summary, the microbiological
analysis demonstrated that Pit O contained a diverse microbial population.
8610H/1-FINAL REPORT 2-1
-------
APPROX. 100ft.
APPROX.
100ft.
-ri
SI,.
+1
Sit. I
+ 1
aJ
(7)CH 0139
(8) CH 0140
(9) CH 0141
(4) CH 0135
(5) CH 0136
(6) CH 0137
(1) CH 0132
(2)CH 0133
«»£H 0134/
^r"
1
15ft.
1
SAMPLE DESIGNATION
SECTION
OFFSITE FIELD BLANK
(10) CH0138
(LOCATED 400 FEET
EAST OF PIT O>
FIGURE 2-1
Pit O Sampling Location!
C OVA
i
BP.IQ SITE TASK FORCE
BRIO PROCESS AR
PIT O SAMPLING LOCAi.
861014/1-FINAL REPORT
2-2
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TABLE 2-1
Pit O Organic Constituent Analysis
Baseline Characterization
Conetntrttloni (n itf/| (ppk)
S»pl1nf locitfan
X
1-5
5-U
10-15
Otpth (ft)
0-$ S-10 11-1$
I-S
$-11
tl-IS
SWPU
CHOI 12 CHOI)}
CMOIX
CHIOS CXOIH 0(01)7 CMIDI CMOI4I W0141
KTOKES
lc*tan*
2-OutlMM
SHMT-OU1II CKlOlimTEO
KYtttUIOONS
CMorofor*
R*tKy1*"« CMorldf
l,2-Oichloro«th
UN 001(4}$) OOL(4SI) 001(440) 001(4)}) 101(411)
101(44$) 101(4)1) Mt(l2S) MK.(ISI) 101(440) 101(4)2) 001(471)
*.(!) Ml(l)
OL(I) OOL(I)
OOL(I) 101(1)
OL(44I) OOL(4)2) 001(411)
001(440) 001(412) 001(411)
UN
OOL(1) UN*
H.(4H) OOL(IIN)
001(1) OOL(4SI)
440 001(44$) OX(4)I) OX(42S) OOL(4SI) 001(440) 001(41}) 101(477)
SI) Od(4)$) 101(440) 001(44$) 001(4)1) OOL(42S) OOL(ISI) 101(140) 101(4)2) 001(111)
001(4}$) 001(440) OOL(44S) 001(4)1) 001(41$) OOL(ISI) HL(III) OX(4))) 001(411)
MIES: (<) TM( r«utt MI ttlwi 1n* dilution tt t
(k) * AUo Itunt In klmk. >o««1H«/»rofc«kU MntMtMt<« tf iM»U
(c) ikav* u*nt
-------
TABLE 2-2
Pit O Microbial Evaluation
Enumeration of Aerobic Heterotrophic Microorganisms
Sample Number
Location
CHOI 32
CHOI 33
CHOI 34
CH0135
CHOI 36
CHOI 37
CHOI 39
CHOI 40
CH0141
CHOI 38
Y
Y
Y
X
X
X
z
z
z
Off -Site
Sample Depth
0 -
5 -
10-
0 -
5 -
10-
0 -
5 -
10 -
0 -
5ft
10ft
15 ft
5 ft
10ft
15ft
5ft
10ft
15 ft
2ft
Number of Microorganisms
Per Gram Wet Weight of Soil
x s.d.
1.75 x
1.87 x
1.21
9.20
7.09
2.31
4.79 x
5.97 x
1.00 x
1.28 x
104
103
103
105
105
10*
106
106
1.66 x 103
9.24 x 102
102
D4
103
X
3.21 x
1.67 x
1.15 x ..
3.21 x 102
2.08 x 104
8.19 x
3.47 x
2.26 x
103
103
105
Notes: CH0138 is the field background sample.
x » Mean
s.d. - Standard Deviation
861014/l-FINAL REPORT
2-4
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Chapter 3
FEASIBILITY OF BIODEGRADATION
-------
3. FEASIBILITY OF ON-SITE BIODEGRADATION
3.1 Introduction
Existing information on the biodegradability of the organic compounds detected in
Pit O was reviewed. The object of this literature review was to assess the
potential for using solid-phase biodegradation to destroy the organic constituents
found in Pit O and elsewhere at the Brio/DOP site.
The organic compounds found at Pit O can be grouped into the following categories:
o Ketones (e.g., acetone and 2-butanone)
o Short-chain chlorinated hydrocarbons (e.g., chlorinated ethanes)
o Chlorinated aromatic hydrocarbons (e.g., chlorobenzenes)
o Aromatic hydrocarbons (e.g., phenanthrene)
'3.2 Degradation Processes
Several processes influence the fate of organic compounds in solid-phase biodegrada-
tion systems. These include physical, chemical, and biological processes such as air
stripping, photooxidation, leaching, absorption, desorption, chemical oxidation,
hydrolysis, and biological absorption and metabolism. The physical, chemical, and
biological properties of the organic constituents and the soil interact with environ-
mental variables to influence the fate of the compounds in the soil environment.
This section discusses the parameters that affect solid-phase biodegradation, and the
expected rates of removal for different types of organic compounds by solid-phase
biodegradation processes.
3.2.1 Physical and Chemical Parameters. The importance of air stripping as a
mechanism for removing compounds during solid-phase biodegradation
processes is directly related to the volatility of the compounds being
treated. For example, air stripping is an important mechanism for
removing volatile compounds such as short-chain chlorinated hydro-
carbons, but it is not an important mechanism for removing compounds
that have low volatility. Operational procedures such as tilling will
increase the air stripping of compounds.
The importance of photooxidation depends upon the location of the
compound. For example, compounds are subjected to photoiytic action
when they are at the soil surface and exposed to light, but not when
they are incorporated into the soil, since sunlight does not penetrate the
soil surface.
Leaching, absorption, desorption, hydrolysis, and chemical oxidation
processes til influence the fate of compounds in solid-phase biodegrada-
tion systems. Absorption is a very important parameter in determining
the fate of compounds in solid-phase biodegradation systems. It influ-
ences the rate of volatilization, diffusion, and leaching, as well as the
availability of the compounds to microbial or chemical degradation. It
should be noted that these parameters are usually not differentiated from
biological processes, and consequently it is difficult to quantify how much
861014/1-FINAL REPORT 3-1
-------
they contribute to compound removal in solid-phase biodcgradation
systems.
3.2.2 Biological Parameters. Microbial processes are usually considered the
most important means for removing compounds in solid-phase biodcgrada-
tion systems. The following parameters influence microbial activity, and
consequently the rate of compound removal in solid-phase biodegradation
systems:
Available Nutrients. The availability of oxygen is one of the most
important parameters influencing the performance of solid-phase biodegra-
dation processes, since aerobic microorganisms (microorganisms which
require oxygen for growth) are primarily responsible for degrading
constituents in solid-phase biodegradation processes. Soil has a limited
oxygen supply which is determined by rate of oxygen diffusion from the
atmosphere into the soil. If the rate of oxygen consumption by the soil
microflora exceeds the rate at which the oxygen supply can be reple-
nished by diffusion, oxygen will become limiting, and the rate of com-
pound removal will decrease. Oxygen diffusion rates vary with soil
texture and structure, soil moisture content and hydraulic loading rates.
Solid phase biodegradation areas are usually tilled at regular intervals to
optimize soil aeration. In addition, the soil moisture content of solid-
phase biodegradation areas usually is maintained at 10% to 20% to prevent
saturation and the development of anaerobic conditions.
Nutrients such as nitrogen and phosphorous are usually present at low
concentrations in soils, and consequently microbial activity is limited.
The addition of these nutrients stimulates microbial activity and increases
the rate of compound removal. Nitrogen and phosphorous are usually
added to the soil in solid-phase biodegradation facilities to produce
carbon:nitrogen:phosphorous ratios of approximately 50:2:1, which is the
optimum ratio for biodegradation.
Additional carbon and energy sources (e.g., manure) are sometimes added
to soils that have low organic carbon contents. The additional carbon
sources usually stimulate the growth of microorganisms which cannot
utilize the organic constituents of interest as food sources, but which can
modify the compounds while growing on the additional carbon sources.
This process is referred to as co-oxidation, and it is due to the non-
specificity of the enzymes used by microorganisms to metabolize food
sources. Caution should be exercised when adding additional carbon and
energy sources to solid-phase biodegradation systems, as this practice can
shift the microbial population from one which preferentially degrades the
compounds of interest to one which preferentially degrades the added
carbon sources and thus reduces the rate of compound removal.
Soil oH. While different types of microorganisms have different pH
values for maximum activity, the optimum pH range for microbial activity
in soils is usually considered to be 6.5 to 8.5, with most microorganisms
preferring a neutral pH (Parr fl al., 1983). The solubility and/or
availability of nutrients and compounds can also be influenced by pH.
861014/1-FINAL REPORT 3-2
-------
Soil Temperature. While there are microorganisms that can grow at
temperatures as low as 4°C and greater than 100°C, the optimum
temperature range for microbial activity in soils is considered to be 15°C
to 35°C. If the degradation rate at a specific temperature is known,
rates for other temperatures can be estimated from the following equation
(Overcash and Pal, 1979):
K2 - iq x 1.08
-------
Recently, Nelson and his colleagues (1986, 1987) reported the isolation of
an aerobic bacterium which could biodegrade trichloroethene. One or
more enzymes of an induciHe pathway for aromatic degradation were
responsible for the degradation of the trichloroethylene.
The chemical characterization also demonstrated that Pit O contains
methylene chloride, bis(2-chloroethyl)ether and hexachlorobutadiene.
These chlorinated hydrocarbons are biodegradable. Jhaveri and Mazzacca
(1983, 1985) have reported the aerobic biodegradation of methylene
chloride. Tabak and his colleagues (1981) reported 100% degradation of
bis(2-chloroethyl)ether and hexachlorobutadiene in flask cultures in the
laboratory.
3.3.3 Chlorinated Aromatic Hydrocarbons. The baseline chemical characteriza-
tion demonstrated that Pit O contains the following chlorinated aromatic
hydrocarbons: chlorobenzene, 1,2-dichlorobenzene, 1,3-dichlorobenzene,
1,4-dichlorobenzene, 1,2,4-trichlorobenzene, hexachlorobenzene, and 2-
chloronaphthalene. All of the chlorinated benzene compounds have been
reported to be biodegradable. For example, Tabak and his colleagues
(1981) reported degradation of these compounds in flask cultures in the
laboratory. Bouwer and McCarty (1985) reported the biotransformation of
chlorinated benzenes in acetate-grown biofilms. Tabak and his colleagues
(1981) also reported significant degradation of 2-chloronaphthalene in
flask cultures in the laboratory.
3.3.4 Aromatic Hydrocarbons. The baseline chemical characterization demon-
strated that Pit O contains the following aromatic hydrocarbons: ethy-
Ibenzene, o-, m-, and p-xylenes, styrenes, 2-methylnaphthalene, phenan-
threne, anthracene, fluorene, and fluoranthene. The microbial degradation
of these compounds is well documented in the literature. There have
been numerous reports on the ability of microorganisms to utilize
benzenes and substituted benzenes, naphthalenes and alkylnaphthalenes,
phenanthrene, and anthracene as sole sources of carbon and energy
(Cerniglia, 1984). Bouwer and McCarty (1985) reported the biotransforma-
tion of styrene in acetate-grown biofilms. Sielicki and his colleagues
(1978) reported aerobic mineralization of styrene in landfill soil. Tabak
and his colleagues (1981) have reported significant degradation of fluorene
and fluoranthene in flask cultures in the laboratory. Although the more
complex 4 and 5 ring compounds have not conclusively been proven to be
utilized by microorganisms as sole sources of carbon and energy, these
compounds can be co-oxidized (Perry, 1979).
3.4 Half-Life Values
The degradation of most organic compounds in the soil follows a first-order
reaction. This implies that at any given time, the rate of degradation is pro-
portional to the concentration of the compound in the soil (Figure 3-1). First order
kinetics generally apply where the concentration of the compound being degraded is
low relative to the biological activity in the soil. At very high concentrations,
Michaelis-Menten kinetics seem to apply and the rate of degradation changes from
being proportional to the concentration of the compound to being independent of
concentration (Kaufman, 1983).
861014/1-FINAL REPORT 3-4
-------
FIGURE 3-1
Typical Degradation Kinetics
O
UJ
o
O
o
z
s
o
o
CONTAMINANT CONCENTRATION
VS.
TIME
1st. ORDER
LOGRITHMIC DECAY
2nd. ORDER
MICHAELIS - MENTEN
DECAY
TIME
Half-life is the term most frequently used to report the rate at which compounds
are removed in solid-phase biodegradation systems and includes losses due to
physical, chemical and biological processes. It is defined as the time required for
the concentration of a compound to decrease to one half of its initial value.
Reported half-life values for the compounds in Pit O range from 5 to 298 days
(Table 3-1). It should be emphasized that most of these half-life values were
determined under different operational conditions. This explains the wide variation
in reported half-life values, and emphasizes that the time required to remove a
compound is directly related to the operational conditions employed in the solid-
phase biodegradation system. Nonetheless, the data does provide a general indica-
tion of expected rates of removal for compounds in solid-phase biodegradation
systems.
861014/1-FINAL REPORT
3-5
-------
TABLE 3-1
Reported Half-Life Values for Compounds
In Soil Treatment
Half-Life
Compound (Davs)
Ethylbenzene 5 to 9
Chlorobenzene 7
o-,m-, and p-xylenes 8 to IS
Naphthalene 24 to 39
2-methylnaphthaIene 46
Phenanthrene 69 to 298
Anthracene 28 to 298
Fluoranthene 44 to 182
Source: Sims, 1986; Ryan, 1986; unpublished data
Ecova is not aware of any information on half-life values for ketones and short-
chain chlorinated hydrocarbons in solid-phase biodegradation systems. Indeed, given
the volatility of these compounds, it would appear that air str ng (Section 3.2.1)
may be the most important mechanism for their removal. Li ise, Ecova is not
aware of any information on half-life values for bis (2-chlor.-thyl) ether, hexa-
chlorobutadiene, styrenes, 1,2-, 1,3-, 1,4-, 1,2,4-, and hexachlorobenzenes; and 2-
chloronaphthalene. However, given the ease with which these compounds can be
biodegraded and/or air stripped, they probably have short half-life values.
3.5 Conclusion
The literature review demonstrated that all of the organic constituents detected in
Pit O- were amenable to solid-phase biodegradation. In order to substantiate this
conclusion, a bench-scale evaluation was undertaken to determine the biodegrad-
ability of the organic compounds found in Pit O.
861014/1-FINAL REPORT 3-6
-------
Chapter 4
BENCH-SCALE TREATABILITY EVALUATION
-------
4. BENCH-SCALE TREATABILITY EVALUATION
4.1 Introduction
The review of the existing information on the biodegradability of the organic
constituents present in Pit O demonstrated that all of the compounds could in
theory be degraded. The object of the bench-scale treatability evaluation was to
demonstrate that biodegradation could indeed be used to destroy the organic
compounds present in Pit O.
4.2 Pit O Microbial Characterization
The level of existing microbiological activity within Pit O was determined by using
standard plate count techniques. The data demonstrated that Pit O contained a
diverse microbial population. The numbers of aerobic heterotrophic microorganisms
in Pit O ranged from 103 to 105 colony forming units per gram wet weight of soil.
Higher numbers of microorganisms were present at the surface than in the sub-
surface, which is typical since more oxygen is available to microorganisms at the
air/soil interface (Section 2.4).
In addition to determining the level of existing microbial activity within Pit O,
chemical characterization was performed to determine the effect of parameters such
as pH, nutrient (nitrogen and phosphorous) availability, and metal concentrations on
the existing microbiological activity. These analyses demonstrated that the concen-
trations of metals in Pit O were not high enough to inhibit microbial activity.
However, the low concentrations of nitrogen, phosphorous, and oxygen in Pit O
probably limited the biodegradation of the organic constituents. pH was within the
range conducive to microbial activity (Appendix C).
4.3 Bench-Scale Microcosm Evaluation
A series of microcosms was established to assess if biodegradation could be used to
destroy the organic constituents present in Pit O soil. These microcosms are briefly
described below.
o Control Microcosm. This microcosm contained Bushnell-Haas medium plus 0.5
percent (w/w) of sodium azide, which inhibits the growth of microorganisms.
This azide-killed control therefore was designed to determine the impact of
non-biological processes, such as air stripping, on the concentration of con-
stituents.
o Inorganic Nutrient-Adjusted Microcosm. This microcosm contained Bushnell-
Haas medium which provided the microorganisms with the inorganic nutrients
they required for growth. These nutrients (primarily nitrogen and phos-
phorous) are usually present at growth-limiting concentrations in the environ-
ment. By providing ample nutrients for microorganism growth, this microcosm
evaluated the effectiveness of indigenous organisms in degrading the organic
constituents found in Pit O.
o Inorganic and Organic Nutrient-Adjusted Microcosm. The third microcosm
contained Bushnell-Haas medium plus 0.05% (w/v) peptone yetst extract. The
additional carbon sources (peptone and yeast extract) stimulated the growth of
861014/1-FINAL REPORT 4-1
-------
microorganisms which could not utilize the organic compounds present in Pit O
as food sources, but which could modify these constituents while growing on
the additional carbon sources. This process is referred to as co-oxidation, and
it is due to the non-specificity of the enzymes used by microorganisms to
metabolize food sources. This microcosm evaluated the effect of adding co-
substrates on the degradation of organic compounds in Pit O.
o Ecova Inoculum Microcosm. The final microcosm contained Bushnell-Haas
medium plus an Ecova inoculum at a concentration of approximately 1 x 10°
microorganisms per ml. The inoculum was composed of microorganisms from
the Ecova culture collection which potentially could degrade the compounds
present in Pit O. This microcosm was designed to determine the effect of the
Ecova inoculum on organic compound concentrations. If no microorganisms had
been isolated from Pit O which could degrade the compounds, this inoculum
could have been used to seed the pilot test.
The concentrations of volatile and semi-volatile compounds in the microcosms were
determined initially and again after 28 days of incubation at 2S°C on a rotary
shaker (Appendix B). Microbial growth was assessed by enumerating the numbers of
microorganisms present in the microcosms on days 0, 7, 14, 21, and 28 (Appendix J).
Microbiological Activity in Microcosms
During the first 7 to 14 days of incubation, the numbers of microorganisms in the
inorganic and organic nutrient-adjusted cultures increased from one-thousand to
one-hundred-thousand-fold, indicating that the growth of the microorganisms could
be stimulated by the addition of inorganic and/or organic nutrients (Figure 4-1).
Furthermore, the data indicated that the concentrations of the organic constituents
in the microcosms did not inhibit the growth of the microorganisms. The numbers
of microorganisms in the azide-labeled control remained constant during the
incubation period, indicating that azide effectively inhibited microbial growth
(Figure 4-1).
Removal of Volatile Organic Compounds
There was no significant difference in the percent reduction observed in the
concentrations of the volatile organic compounds in the control and the test
microcosms (Table 4-1). The concentrations of all the volatile compounds except
acetone were reduced by 84% or more. The concentration of acetone was reduced
by 36%; this low removal rate was probably due to the high aqueous solubility of
acetone. The data demonstrated that while the volatile compounds are biodegrad-
able, because of their physical properties, volatilization would be the predominant
mechanism by which these constituents would be removed in the pilot-scale treat-
ment facility.
Biodeeradation of Semi-Volatile Organic Compounds
Bis(2-chloroethyl) ether and phenanthrene were the only semi-volatile compounds
present at detectable levels in the microcosms (Table 4-2). The concentration of
bis(2-chloroethyl) ether was reduced by more than 97%, while the concentration of
phenanthrene was reduced by more than 61%. Microbial degradation was responsible
for the reduction in the concentration of phenanthrene. Both volatilization and
861014/1-FINAL REPORT 4-2
-------
FIGURE 4-1
Pit O Microbial Evaluation
Typical Growth Patterns in Enrichment Cultures
r^
i
s
1
i
o
O
mix aursi
BttPY O Bfftt
Off
BH
BHPY
BHEI
BHA
Bushnell-Haas Medium
Bushnell-Haas Medium plus Peptone/Yeast Extract
Bushnell-Haas Medium plus Ecova Inoculum
Bushnell-Haas Medium plus Azide
861014/1-FINAL REPORT
4-3
-------
TABLE 4-1
Volatile Organic Carbon Compound Concentrations
in Enrichment Culture CH0132
2
O
Sft
*g
M U
K
Q
Ct)
K
X
M
w
X
CO
Q
u
K
X
a.
CO
Q
U
cc
M
CQ
Q
U
cc
M
<
CO
INITIAL
CONC.
COMPOUND
«f
(O M
CO CD
f CO
rt o
m cn
^ 00
10 *
f »H
» >H
co 10
« ID
co co
* *
o m
CO O
»» -^
^ o>
to cn
CM CO
CO rt
« cr>
CM Ol
CM ID
CO
r»
ID f
-1 ID
01 cn
n n
CM cn
ij
Q
n
t» i»
01 +
CM ID
*
CM
CHLOROFORM
MEHTYLENB
CHLORIDE
f
4
V
CO
^*
IO
CM
u
o
CO
r-
«
4
1,1-DICHLORO
ETHENB
ID co r-
CO CD ID
CM CO ID
CO CD Ol
Ol 01 Ol
» m K>
CM CM CM
Q O O
CO CO CO
CM CO ID
CO CD 01
cn cn en
m m m
CM CM CM
Q O Q
CO CO CO
« CO Ol
cn co o
cn cn cn
IO CM
a o
r* CM 10
«^
£
(Q
CM CO O>
CO CO IO
01 Ol Ol
CM CM t»
ij tJ
Q Q
COCO
« « O
c» o> n
now
«H CM W
TRICHLOROETHENE 1
TETRACHLOROETHENE
CHLOROBENZENE
10 O
ID f
10 O
ID fi
01 CO
10 10
CM CM
0 D
co n
in o
ID t-
01 co
in «
CM CM
o a
co a
0 O
BDL(2S) 96
BDL(25) 87
10 0
to t^
O) CO
CM CM
So1
CQ (Q
a M
«-i 01
! n
1
TOTAL XYLENES
STYRENE
CO CD
ID IO
CO CD
ID IO
Ol O)
« 10
CM CM
0 Q
n co
co co
ID <0
Ol 01
10 in
CM CM
aa
ma
CO CO
BDL(2S) 96
BDL(23) 96
CO CO
ID ID
en 01
CM CM
UtJ
aa
CQ a
00
« CO
1- 1-
BENZENE 1
TOLUENE
»i
6
H
J
C
wl
V
itimated Detec.
u
Limit
g
H
V
u
V
u
a
I
r~»
w
NOTE: BDL(X) 1
861014/1-FINAL REPORT
4-4
-------
TABLE 4-2
Semi-Volatile Organic Carbon Compound Concentrations
in Enrichment Culture CH0132
z
0
bi H
> 0
< D
a
* U
.
a
M
K
it
M
w
X
CO
.
Q
u
K
*
^4
a*
X
o
Q
M
OS
X
a
Q
M
OS
^
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CQ
u
<
M U
H Z
M O
Z U
IH
a
z
J3
o
a,
£
O
O
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0
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o
0
a
^
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a
CO
.
u
H
O
Z
861014/1-FINAL REPORT
4-5
-------
biodegradation were responsible for the reduction in the concentration of the bis(2-
chloroethyl)ether. There was no significant difference in the percent reduction
observed in the different test microcosms, indicating that all of the treatment
options evaluated in the microcosms promoted similar rates of biodegradation of the
semi-volatile constituents. Reductions in the concentrations of the semi-volatile
compounds in control microcosms was an experimental artefact due to insufficient
inhibition of microbial activity by azide.
The data demonstrated that the semi-volatile compounds in Pit O soil were amenable
to biodegradation, and that biodegradation would be the mechanism by which these
compounds would be destroyed in the proposed pilot-scale treatment facility.
4.4 Summary and Conclusions
The bench-scale treatability evaluation demonstrated that the semi-volatile organic
constituents present in Pit O soil were amenable to biodegradation, and that
biodegradation would be the mechanism by which these compounds would be
destroyed. The evaluation also demonstrated that while the volatile compounds are
biodegradable, because of their physical properties, volatilization would be the
mechanism by which these constituents would be removed in the proposed treatment
facility.
Since effective removal of volatile organic compounds and biodegradation of semi-
volatile compounds was demonstrated by this bench-scale testing, Ecova recom-
mended the construction and operation of a pilot-scale, solid-phase biodegradation
facility in order to demonstrate the effectiveness of this process under field
conditions.
861014/1-FINAL REPORT 4-6
-------
Chapter 5
ON-SITE PILOT SCALE TREATMENT FACILITY
-------
5. ON-SITE PILOT-SCALE TREATMENT FACILITY
5.1 Introduction
The bench-scale treatability evaluation demonstrated that a biological treatment
system could be used to remove the organic constituents present in Pit O soil.
Consequently, in January of 1987 Ecova initiated an on-site pilot-scale demonstra-
tion of solid-phase biodegradation. An above-ground, fully enclosed treatment
facility was constructed. The facility was designed to simulate the system charac-
teristics and operating conditions of a full-scale solid-phase biodegradation system.
The bench-scale treatability evaluation indicated that volatilization would be the
most important mechanism for removing the volatile organic constituents present in
Pit O soil. Consequently, the pilot-scale facility contained an activated carbon
system to control the release of the volatile organic compounds.
The bench-scale treatability evaluation also demonstrated that the soil in Pit O
contained a diverse microbial population, and that this population could degrade the
semi-volatile organic constituents present in the soil. The object of the solid-phase
treatment process was to stimulate the existing microbiological population to destroy
the semi-volatile constituents.
5.2 Treatment Facility Construction and Operation
The pilot-scale solid-phase treatment facility consisted of a lined soil treatment area
with a leachate collection system; a water and nutrient distribution system; an
emission control system; a microbiological management system (including a fermenta-
tion vessel); a greenhouse type enclosure; and support facilities. Construction and
operation details are presented in Appendix E.
The soil treatment area was divided into four lanes so that different methods of
optimizing microbial activity and biodegradation rates could be evaluated. The four
treatment lanes are described below.
o Control. The control lane was established to provide a baseline for evaluating
the effectiveness of the three treatment options. This lane received only
tilling and water additions.
o Nutrient-Adjusted. The inorganic nutrient-ady*sted lane determined the
biodegradative abilities of the existing microorgarusms from Pit O. Inorganic
nutrients (nitrogen and phosphorous) were added to the lane to stimulate the
activity of these microorganisms. This treatment process was designed to
determine the rate at which the indigenous microbiological activity could
degrade organic compounds with only the addition of oxygen and inorganic
nutrients.
o Inoculated. This lane was inoculated at the start of operations with high con-
centrations (103 to 104 cfu per gram weight of soil) of microorganisms isolated
from Pit O. An inoculum containing indigenous microorganisms was developed
in the on-site fermentation vessel and applied to the soil with inorganic
nutrients. This treatment process was designed to determine if the rate of
861014/1-FINAL REPORT 5-1
-------
organic compound degradation could be increased by augmenting the existing
microbial activity from Pit O, and by adding oxygen and nutrients.
o Multiple-Inoculated. After initial inoculation, this lane was subsequently
inoculated at approximately ten day intervals using inocula developed from soil
removed from the lane and water from the leachate collection system. This
treatment was designed to determine if the rate of removal of organic
constituents could be accelerated by increasing the frequency of application of
microorganisms in addition to adding nutrients and oxygen.
The pilot test facility was operated for 94 days. The soil in the treatment facility
was tilled daily to optimize contact between microorganisms and the organic
constituents, and to ensure adequate aeration for microbial activity. Tilling also
facilitated the air stripping of volatile organic compounds. Soil moisture content,
soil temperature, and soil pH were monitored to ensure that they remained within a
range conducive to microbial activity. (Facility operation is described in Appendix
E.)
The effectiveness of the various treatment options was determined by measuring the
concentrations of volatile and semi-volatile organic constituents in the treatment
lanes. Microbiological activity was monitored by using standard plate count and
radiotracer techniques.
5.3 Initial Treatment Facility Conditions
Concentrations of Volatile Organic Compounds. The predominant volatile organic
compounds detected in the pilot-scale treatment facility were ethylbenzene, styrene,
and toluene. These compounds were detected at maximum concentrations of
4,400,000 ppb, 240,000 ppb, and 510,000 ppb, respectively. Methylene chloride and
1,1,2-trichloroethane were also detected but at lower concentrations. For example,
methylene chloride concentrations ranged from 530 ppb to 20,000 ppb, and 1,1,2-
trichloroethane concentrations ranged from 520 ppb to 110,000 ppb (Tables 5-1 to
5-4). .
Concentrations of Semi-Volatile Organic Compounds. Phenanthrene was the
predominant semi-volatile organic constituent detected in the treatment facility.
Phenanthrene concentrations ranged from 440 ppb to 170,000 ppb and the average
phenanthrene concentration was 36,300 ppb. Methyl naphthalene and naphthalene
were also detected in the pilot test facility. 2-Methylnaphthalene concentrations
ranged from 6,200 ppb to 170,000 ppb. and the average concentration was 50,700
ppb. Naphthalene concentrations ranged from 130 ppb to 96,000 ppb, and the
average concentration was 19,500 ppb (Tables 5-5 to 5-8).
Microbiological Activity. The initial concentrations of microorganisms in the pilot
test area ranged from 104 to 107 colony forming units (cfu) per gram wet weight of
soil (Table 5-9). 14C-glucosc radiotracer studies indicated that the activity of these
microorganisms varied widely with location in the treatment facility. The initial
percent mineralization of 14C-glucose to 14C<>2 ranged from 0% to 50%, and the
average was 21% (Table 5-10). 14C-phenanthrene radiotracer studies indicated that
a phenanthrene biodegradation potential did exist in the treatment facility, but that
861014/1-FINAL REPORT 5-2
-------
TABLE 5-1
Removal of Volatile Organic Compounds
in the Control Lane
(Concentrations in ppb)
COMPOUND
Acetone
2-Butanone
Methylene Chloride
1,1, 2-Trichloroe thane
1,1,2, 2-Tetrachloroe thane
Total Xylenea
Cthylbenzene
Styrene
Toluene
QUADRANT
1
2
3
4
AV. (XRED)
1
2
3
4
AV. (XRED)
1
2
3
4
AV. (KRED)
1
2
3
4
AV. (XRED)
1
2
3
4
AV.(XRED)
1
2
3
4
AV.(XRED)
1
2
3
4
AV. (XRED)
1
2
3
4
AV.(XRED)
1
2
3
4
JAN 26
6.900
18,000
19,000
17,000
15,22S(-)
9,500
24.000
22,000
17,000
18.12S(-)
1600
3,800
6.400
4,300
4,02S(-)
2,500
14,000
33,000
7,100
14,150(-)
BDL(S.OOO)
4,600
5,100
4,000
4675(-)
12,000
BDL(IO.OOO)
23,000
6.900
12.97S(->
190,000
200.000
40,000
83.000
128,250(-)
1 1 . 000
7.600
18.000
4.700
10,325(-)
15,000
34.000
44.000
11,000
FEB 16
46
BDL(18)
BDL(49)
34
37(99.8)
BDL(46)
BDL(18)
BDL(49)
BDL(48)
40(99.8)
34
11
29
29
26(99.4)
17
22
380
250
167(98.8)
BOM 23)
BDL(S»
BDL(24)
BDM24)
20(99.6)
BDL(23)
BDM9)
BDM24)
BDM24)
20(99.8)
660
62O
85
54
355(99.7)
34
57
BDL(24)
BDM24)
35(99.7)
24
31
BDL(24)
BDL(24)
MAR 25
62
49
31
36
45(99.7)
31
38
31
31
33(99.8)
18
16
12
13
15(99.6)
22
19
71
28
35(99.8)
BDL(5)
BDL(6)
3
BDL(6)
5(99.9)
BDt,(S)
BDL(6)
BDL(5)
BDL(6)
6(>99.9)
4
2
BDL(5)
BDL(6)
4(>99.9)
1
BDL(6>
1
BOL(6)
4(>99.9)
2
BOL(6)
BOL(S)
BDL(6)
APR 30
27
10
27
27
23(99.9)
S3
36
36
35
40(99.8)
9
13
15
13
13(99.7)
27
11
210
75
81(99.4)
BDL(S)
BDL ( 6 )
7
BDL(5)
6(99.9)
13
3
BDL(S]
BOMS;
7(99.9
290
28
6
7
73(99.9
17
2
BDLC
BDL(!
7(99.5
2:
AV.(XRED)
26,000(-) 26(99.9) 5(>99.9) 7(>99.
861014/1-FINAL REPORT
5-3
-------
TABLE 5-2
Removal of Volatile Organic Compounds
in the Nutrient-Adjusted Lane
(Concentrations in ppb)
COMPOUND
Acetone
Methylene Chloride
1 , 1-Dlchloroethene
Total Xylenes
Ethylbenzene
Styrene
Toluene
QUADRANT
1
2
3
4
AV. (XRED)
1
2
3
4
AV.(XRED)
1
2
3
4
AV.(XRED)
1
2
3
4
AV.(XRED)
1
2
3
4
AV.(XRED)
1
2
3
4
AV.(XRED)
1
2
3
4
JAN 26
9,300
8 ,300
3,400
8,600
7400(-)
1,900
1,800
530
BDL(IO.OOO)
3,558(-)
BDL(S.OOO)
2,300
8.900
14,000
7,5SO(-)
BDM3.000)
4,000
960
12.000
S,490(-)
170.000
120,000
35,000
580,000
226.250(-)
2.500
6,500
2,000
15,000
6.500(-)
5,300
17,000
6,600
49.000
FEB 16
21
48
30
8
27(99.6)
8
21
32
9
18(99.5)
BDL(7)
BDL(8)
BDL(5)
BDL(6)
6(99.9)
BDL(7)
BDL(8)
BDL(S)
BDL(6)
6(99.9)
2
760
3
19
196(99.9)
BDL(7)
28
BDL(5)
3
11(99.8)
BDL(7)
SO
BDL(5)
4
MAR 25
45
58
26
19
37(99.5)
22
13
9
8
13(99.6)
BDL ( 5 )
BDL(6)
BDL(5)
BDL(5)
5(99.9)
BDL ( 5 )
BDL ( 6 )
BDL(5)
BDL(5)
5(99.9)
57
57
BDL(S)
BDL(5)
31(>99.9)
2
3
BDL(S)
BDL(S)
4(99.9)
5
11
1
1
APR 30
68
16
11
16
28(99.6)
36
26
21
18
25(99.3)
BDL(6)
BDL { 6 )
BDL(6)
BDL ( 6 )
6(99.9)
8
2
BDL(6)
2
5(99.9)
21
3
4
6
9(>99.9)
BDL(6)
BDL ( 6 )
BDL(6)
BDL ( 6 )
6(99.9)
4
1
2
2
AV.(XRED)
19.475(-) 17(99.9) 5(>99.9) 2O99.9)
8610I4/1-FINAL REPORT
5-4
-------
TABLE 5-3
Removal of Volatile Organic Compounds
in the Single Inoculated Lane
(Concentrations in ppb)
COMPOUND
Accton*
2-But«nonc
H*thyl*n« Chloride
1 . 1 . J-Trlchloro«than«
1 . l-Dlchloro«th«n«
Chlorobcnztn*
Total Xyl«n««
Cthylb«nz«n*
Sty ran*
B«nx«n*
Tolotn*
QUADRANT
1
2
3
4
AV.(XRED)
1
2
3
4
AV.(XRED)
1
2
3
4
AV.(kRED)
1
2
3
4
AV.(XRED)
1
3
3
4
AV.IXXED)
1
t
3
4
AV. (XRED)
1
2
3
4
AV. (XRED)
1
2
3
4
AV. (XKEO)
1
2
3
4
AV. (XXZD)
1
2
3
4
AV.(XREO)
t
2
3
4
JAN 2*
37.000
ii.OOO
13,000
2.000
SO.OOO(-)
BOL(SO.OOO)
84.000
1! . '0
Si. -JO
42.000(-|
BDU29.000)
20.000
3.700
17.000
19.429(-)
29,000
22.000
110,000
49.000
91.290(-|
21.000
200.000
4.100
BDt(SO.OOO)
99.7791-)
BOL(29,000)
2*. 000
3.400
11.000
19.100I-)
71.000
110.000
,300
110.000
4,329(-)
1,300.000
3.200.000
13O.OOO
4.400.000
2.297.900(-)
71.000
4.00O
00
140.000
104.700(-)
13.00O
a. too
BDLUO.OOO)
39.0OO
1B.200(-)
140.000
400. OOO
13.000
910.000
FIB 1«
7
It
43
4»(»».»)
BDL(14)
BOL(14|
BOt(lS)
BDL(14)
14(>tfl.t)
B
1«
10(tB.B)
BOLI7I
4
4t
21
20(>»».»)
BDL(7)
BDU7)
BOL(C)
BOL(7)
7(>M.«)
BOL(7>
BOL(7)
4
4
(>. B)
4
BOL(7)
BOL(«)
BOL(l)
(>*.»
B
42
B
B
!«(>.)
S
4
9
a
B(>BB.B)
Wt(7)
BOU7)
nut)
M.I7)
7(>«t.B)
BBL(7)
4
BOL(«)
01(7)
MA* 29
BOL(ll)
21
29
13
!(>»».)
31
22
22
IB
24(BB.B)
9
19
IB
12(«».»|
74
91
S
39IBB.B)
BOL(B)
2
BOMO
BOL(B)
9OBB.B)
BDL(B)
3
4
BOC«)
9OBB.B)
19
BDLI9)
BDL(«)
BOL(B)
BOBB.B)
ICO
1
BOL(B)
1
97OBO.B)
B
3
BOMB)
B8t(B)
(>. t)
BOL(«)
BM(C|
BOL(9)
BOt(B)
90BB.BI
4
IB
t
a
APR 30
10
9
It
12
11(>»9.»)
39
33
39
37
39I9B.B)
11
19
12
10
12(99. B>
37
17
99
130
73(99.9)
BDM9)
BOMB)
BOU9)
BDL(S)
»(>»».»)
BOM 9)
BDL(9)
9
9
«(>».«)
19
9
B0t(»)
4
Bon.B)
(90
110
B
a*
10BOBB.B)
IB
9
BOKB)
3
7(>t».t>
BOKB)
B0L(«)
BBLIB)
1
4OBB.B)
29
9
2
4
AV.(XRED)
29S.790(-> 9(>BB.B) 9(>BB.B) 10(>B9.9)
861014/1-FINAL REPORT
5-3
-------
TABLE 5-4
Removal of Volatile Organic Compounds
in the Multiple-Inoculated Lane
(Concentrations in ppb)
COMPOUND
QUADRANT
JAN 26
FEB 16
MAR 25
APR 30
Acetone
2-Butanone
1.1, 2-Trichloroeth*ne
Total Xylenes
Ethylbenzene
Styrene
Benzene
Toluene
1
2
3
4
AV. (XRED)
1
2
3
4
AV.(XRED)
1
2
3
4
AV.(XRED)
1
2
3
4
AV. (XRED)
1
2
3
4
AV.(XRED)
1
2
3
4
AV. (XRED)
1
2
3
4
AV. (XRED)
1
2
3
4
3,100
73,000
3,400
BDLC3.000)
23.125(-)
3.700
76,000
4,900
BDL (13, 000)
24,400(-)
520
BDL (50, 000)
77.000
3,000
36.630(-)
550
98.000
BDL (500)
11.000
27,512(-)
24,000
2,000,000
350
350,000
593.588(-)
990
140.000
BDL (500)
11.000
38.122(-)
210
23.000
BDL (500)
1,700
6,352(-)
1,700
230,000
170
29.000
BDL(14)
12
19
34
20(99.9)
BDL (14)
BDL(IO)
BDL (10)
BDL (10)
11099.9)
BDL(7)
2
14
14
9(>99.9)
BDL ( 7 )
BDL(S)
BDL(S)
BDL(5)
6(>99.9)
6
50
12
16
21(>99.9)
BDL(7)
3
2
BDL(5)
4(>99.9)
BDL(7)
BDL(S)
BDL(S)
BDL(S)
6(99.9)
BDL(7)
3
2
BDL ( 5 )
16
28
21
21
22(99.9)
20
32
24
31
27(99.9)
2
23
63
22
32(99.9)
BDL(5)
BDL ( 5 )
BDL(5)
21
9(>99.9)
BDL(5)
11
19
110
36(>99.9)
BDL ( 5 )
3
2
6
4(>99.9)
BDL ( 5 )
BDL(S)
BDL(5)
BDL(S)
5(99.9}
1
5
BDL(S)
60
11
22
14
12
15(99.9)
36
' 33
39
20
32(99.9)
3
15
180
19
54(99.8)
3
20
BDL ( 6 )
3
8O99.9)
28
280
6
38
88(>99.9)
2
20
BDL(6)
2
8(>99.9)
BDL ( 5 )
BDL(6)
BDL(6)
BDL(S)
6(99.9)
2
12
1
3
AV.(XRED) 65,218(-) 4(>99.9) 18(>99.9) 4(>99.9)
861014/1-FINAL REPORT
5-6
-------
TABLE 5-5
Degradation of Semi-Volatile Organic Compounds
in the Control Lane
(concentrations in ppb)
Compound
Phenanthrene
Quadrant
1
2
3
4
Average (% Red.)
January 26 February 16
34,000
8,400
11,000
58.000
27,850(-)
12,000
31,000
13,000
16.000
March 25
19,000
11,000
9,800
8.30Q
April 30
9,000
4,100
7,100
2.700
18,000(35.4) 12,025(56.8) 5,725(79.4)
Naphthalene
1
2
3
4
Average (% Red.)
14,000
3,400
4,400
18.000
9,950(-)
830
980
150
810
692(93.0)
50
BDL(380)
50
220
130
115
131(98.7) 131(98.7)
861014/1-FINAL REPORT
5-7
-------
TABLE 5-6
Degradation of Semi-Volatile Organic Compounds
in the Nutrient-Adjusted Lane
(concentrations in ppb)
Compound
Phenanthrene
Quadrant
1
2
3
4
Average (% Red.)
January 26 February 16
14,000
2,600
3,000
58.000
19,400(-)
13,000
20,000
7,200
12.000
March 25
19,000
10.000
5,600
7.700
5,600
2,300
1,700
1.25Q
13,050(32.7) 10,575(45.5) 2,712(86.0)
Naphthalene
1
2
3
4
Average (% Red.)
4,600
12,000
BDL(6,600)
28.000
12,800(-)
140
350
BDL( 1,650)
BDLM.65Q)
948(92.6)
100
80
70
75(99.4)
170
60
45
2&
91(99.3)
86I014/1-FINAL REPORT
5-8
-------
TABLE 5-7
Degradation of Semi-Volatile Organic Compounds
in the Single-Inoculated Lane
(concentrations in ppb)
Compound
Quadrant
2-Methylnaphthalene 1
2
3
4
Average (% Red.)
January 26 February 16
6,200
170,000
BDL (6,600)
20.000
50,700(-)
310
720
230
420
420(99.2)
March 25
April 30
130
160
160
HP.
142(99.7)
190
65
50
ISO
114(99.8)
Phenanthrene
1
2
3
4
Average (% Red.)
48,000
170,000
4,400
72.000
73,600(-)
11,000
21,000
7,600
13.000
13,150(82.1)
12,000 7,700
11,000 5,600
12,000 5,300
11.600 4.400
11,650(84.2) 5,750(92.2)
Naphthalene
I
2
3
4
Average (% Red.)
22,000
96,000
BDL(6,600)
62.000
46,650(-)
150
430
160
300
260(99.4)
110
160
86
_8J>
109(99.8)
420
115
75
219.
205(99.6)
861014/1-FINAL REPORT
5-9
-------
TABLE 5-8
Degradation of Semi-Volatile Organic Compounds
in the Multiple Inoculated Lane
(concentrations in ppb)
Phenanthrene
1
2
3
4
Average (% Red.)
7,000
40,000
440
50.000
24,360(-)
8,400
19,000
10,000
8.700
11,525(52.7)
11,000 5,900
22,500 6,700
8,950 3,100
9.8QO 5.400
13,062(46.4) 5,275(78.3)
Naphthalene
1
2
3
4
Average (% Red.)
(BDL)(6600)
2.800
130
25.000
8,632(-)
70
200
200
BDLf 1.650)
530(93.9)
BDL(370)
110
60
100
160(98.1)
90
800
55
360
326(96.2)
861014/1-FINAL REPORT
5-10
-------
TABLE 5-9
Initial Microorganism Concentrations
Treatment Cells/g wet wt
Lane of Soil Standard Deviation
CONTROL
Quadrant: 1 5.85 x 104 5.90 x 105
2 1.98 x 105 2.72 x 104
3 1.04 x 106 1.04 x 105
4 2.58 x 104 1.99 x 103
Mean 1.78 x 106 1.81 x 105
INORGANIC
NUTRIENT-ADJUSTED
Quadrant: 1 2.44 x 103 7.00 x 103
2 1.03 x 105 6.08 x 103
3 7.85 x 104 5.51 x 103
4 2.57 x 104 1.90 x 105
Mean 7.49 x 105 5.21 x 104
SINGLE-INOCULATED
LANE
Quadrant: 1 2.76 x 104 1.42 x 103
2 1.49 x 104 2.05 x 103
3 5.89 x 104 4.16 x 103
4 1.55 x 104 5.13 x 102
Mean 2.92 x 104 2.04 x 103
MULTIPLE-INOCULATED
LANE
Quadrant: 1 1.08 x 107 1.76 x 106
2 1.19 x 104 1.53 x 102
3 1.91 x 104 5.42 x 103
4 2.11 x 105 9.64 x 103
Mean 2.76 x 106 4.44 x 105
861014/1-FINAL REPORT 5-11
-------
TABLE 5-10
Initial Percent Mineralization of 14C-Gluco$e
to 14C-CO2
Percent
Treatment '^C-Glucose Mineralization
CONTROL LANE
Quadrant: 1 50.0
2 40.8
3 34.0
4 7.1
Mean 33.0
INORGANIC NUTRIENT
ADJUSTED LANE
Quadrant: 1 29.4
2 0.9
3 1.8
4 38.5
Mean 17.7
SINGLE-INOCULATED
LANE
Quadrant: 1 ,18.0
2 0.1
3 24.3
4 0.0
Mean 10.6
MULTIPLE-INOCULATED
LANE
Quadrant: 1 47.6
2 0.0
3 14.8
4 34.0
Mean 24.1
NOTES: 1. The experimental procedure employed is described in Appendix J.
2. Incubation was for 24 hours at 2S°C.
3. All counts were corrected for non-biological mineralization in controls.
861014/1-FINAL REPORT 5-12
-------
TABLE 5-11
Initial Percent Mineralization of 14C-Phenanthrene
to 14C-C02
Percent
Treatment "C-Phenanthrene Mineralization
CONTROL LANE
Quadrant: 1 17.9
2 25.4
3 28.9
4 3.6
Mean 19.0
INORGANIC NUTRIENT
ADJUSTED LANE
Quadrant: 1 6.9
2 0.0
3 31.7
4 51.5
Mean 22.5
SINGLE-INOCULATED
LANE
Quadrant: 1 0.5
2 1.0
3 45.8
4 0.1
Mean ' 11.6
MULTIPLE-INOCULATED
LANE
Quadrant: 1 42.7
2 0.0
3 40.0
4 2.0
Mean 10.7
NOTES: 1. The experimental procedure employed is described in Appendix J.
2. Incubation was for 7 days at 25°C.
3. All counts were corrected for non-biological mineralization in controls.
861014/1-FINAL REPORT 5-13
-------
this potential was low and extremely variable (Table 5-11). The percent mineraliza-
tion of 14C-phenanthrene to 14CC>2 ranged from 0% to 51.5% and the average was
16%.
5.4 System Performance
Removal of Volatile Organic Compounds. The concentrations of the volatile organic
compounds in the treatment facility were reduced by more than 99% (Tables 5-1 to
5-4). Most of this reduction occurred within the first 21 days of operation of the
pilot test, and was predominantly due to air stripping. It is possible that microbial
degradation of some of the volatile compounds occurred; however, due to the rapid
rate at which these compounds were air stripped from the treatment facility, it was
not considered necessary to evaluate the contribution that biodegradation may have
made to the removal of the volatile organic constituents.
Volatile compounds of both high and low volatility were removed with equal
efficiency. For example, the concentrations of methylene chloride and 1,1,2-
trichloroethane, both highly volatile compounds, were reduced by more than 99%.
The concentrations of ethylbenzene and styrene, both low volatility compounds, were
also reduced by more than 99%.
Two methods were used to estimate the amount of volatile organic constituents air
stripped from the pilot-test treatment facility: the concentrations of volatile
compounds in the air management activated carbon units, and the OVM data
compiled during the pilot-test. The estimated amounts of volatile compounds air
stripped by these methods were 137 kg and 159 kg, respectively. Since more than
99% of the volatile constituents were air stripped during the first 21 days of the
pilot-test, this is equivalent to a removal rate of approximately 7 kg per day.
Biodegradation of Semi-Volatile Organic Compounds. The initial concentrations of
microorganisms in the pilot test area ranged from 104 to 107 cfu per gram wet
weight of soil. During the operation of the pilot test, these numbers increased to
107 to 10* microorganisms per gram wet weight of soil (Table 5-12). The increase
and stabilization in the numbers of microorganisms indicated that the pilot test
operations produced stable microbial communities within the pilot test area.
The initial percent mineralization of 14C-labelled glucose to 14C-labelled carbon
dioxide within the pilot test area was low and extremely variable. During the first
21 days of operation of the pilot test, the percent mineralization stabilized at
approximately 46% and then subsequently decreased to approximately 17% by day 94
(Table 5-13). The initial percent mineralization of 14C-labelled phenanthrene to
'4C-labelled carbon dioxide ranged from 0% to 51.5%. During the 94-day operation
period of the pilot test, the percent mineralization increased to, and stabilized at,
approximately 44% (Table 5-14).
The initial increase and stabilization in the percent mineralization of 14C-glucose
and 14C-phenanthrene indicates that the pilot test operations stimulated and
promoted an even distribution of microbial activity, and in particular, phenanthrene
biodegradation potential, within the pilot test area. The subsequent decrease in
14C-glucose activity suggests that the pilot test operations promoted the establish-
ment of a microbial population which preferentially degraded phenanthrene, the most
861014/1-FINAL REPORT 5-14
-------
TABLE 5-12
Numbers of Aerobic Heterotrophic
Microorganisms in Pilot-Test Treatment Lanes
a
iitti
iiiii
Hill
S3
S- =
«^ « m *>4
?
« s
a: -s.
s'
v
;s
| -s
;r
r
3
! ;iff! l il] i
861014/1-FINAL REPORT 5.15
-------
Treatment
CONTROL LANE
Quadrant: 1
2
3
4
Mean
TABLE 5-13
Percent Mineralization of 14C-GIucose
to 14C-CO2
Percent
^C-Glucose Mineralization
February 16 (Day 21) March 25 (Day 58) April 30 (Day 94)
48.8
43.9
44.0
45.2
45.5
38.9
21.8
24.2
16.9
25.5
17.8
9.7
5.8
6.4
9.9
INORGANIC NUTRIENT
ADJUSTED LANE
Quadrant: 1 44.0
2 48.7
3 48.5
4 48.6
Mean 47.5
41.4
38.8
21.9
19.3
30.4
41.6
25.0
11.6
13.8
23.0
INOCULATED LANE
Quadrant: 1
2
3
4
Mean
48.6
45.6
45.6
39.9
44.9
38.8
29.1
14.5
33.9
29.1
18.6
33.7
7.5
11.2
17.8
MULTIPLE INOCULATED
LANE
Quadrant: 1 48.5
2 45.6
3 45.6
4 42.8
Mean 45.6
43.7
38.8
24.2
33.9
35.2
18.0
21.4
10.7
13.4
15.9
NOTES: 1. The experimental procedure employed is described in Appendix J.
2. Incubation was for 24 hours at 25°C.
3. All counts were corrected for non-biological mineralization in controls.
861014/1-FINAL REPORT
5-16
-------
TABLE 5-14
Percent Mineralization of 14C-Phenanthrene
to 14C-C02
Treatment
CONTROL LANE
Quadrant: 1
2
3
4
Mean
Percent
"C-Phenanthrene Mineralization
February 16 (Day 21) March 25 (Day 58) April 30 (Day 94)
46.4
51.9
31.9
37.7
42.0
37.6
45.1
44.8
43.5
42.8
49.8
39.3
43.2
38.0
42.6
INORGANIC NUTRIENT
ADJUSTED LANE
Quadrant: 1 51.6
2 41.9
3 56.2
4 55.0
Mean 51.2
40.2
42.5
45.2
41.8
42.4
49.7
41.0
49.2
46.9
46.7
INOCULATED LANE
Quadrant: 1
2
3
4
Mean
46.4
31.9
35.7
37.9
38.0
43.1
46.3
42.9
40.7
43.3
48.9
41.3
39.9
44.9
43.8
MULTIPLE INOCULATED
LANE
Quadrant: 1 48.6
2 40.2
3 47.8
4 48.1
Mean 46.2
47.0
38.9
44.2
43.1
43.3
42.7
47.8
43.5
37.1
42.8
NOTES: 1. The experimental procedure employed is described in Appendix J.
2. Incubation was for 7 days at 25°C.
3. All counts were corrected for non-biological mineralization in controls.
\
861014/1-FINAL REPORT
5-17
-------
predominant semi-volatile constituent in the soil. The concentration of phenan-
threne was reduced by 78.3% to 92.2%
Naphthalene and 2-methylnaphthalene were also detected in the treatment facility.
The concentrations of naphthalene and 2-methylnaphthalene were reduced by 96.2%
to 99.8% (Tables 5-5 to 5-8).
Because of the predominance of phenanthrene in the pilot test treatment facility,
this compound was selected to determine the effect of the various treatment options
on the rate of removal of semi-volatile compounds.
Phenanthrene half-life values for the control, nutrient-adjusted, inoculated, and
multiple-inoculated lanes were 40.8 days, 33.0 days, 25.7 days, and 43.3 days,
respectively (Tables 5-15 and 5-16). A statistical analysis of the data demonstrated
that there was no significant difference (p < 0.05) in the rate of phenanthrene
degradation in the different treatment lanes (Appendix H). Initial phenanthrene
concentration was apparently the parameter controlling the rate of phenanthrene
degradation (Table 5-17). The data also suggest that aeration and optimizing
contact between the microorganisms and the phenanthrene were also important
parameters controlling the rate of phenanthrene degradation.
Because there was no significant difference in the rate of phenanthrene degradation
in the different treatment lanes, all of the data was pooled to provide an overall
determination of the rate of phenanthrene biodegradation in the treatment facility.
The average half-life value for phenanthrene in the treatment facility was 33 days,
significantly less than previously reported half-life values for phenanthrene in solid-
phase biodegradation systems, which ranged from 69 days to 298 days.
During the first 21 days of operation, phenanthrene degradation occurred at a
relatively rapid rate. For the remainder of the pilot test the rate of phenanthrene
degradation was approximately linear (Figure 5-1), and the removal rate was
approximately 124 micrograms per kilogram per day. If the rate of degradation
remained linear, approximately 131 days would be required for the phenanthrene
concentration to reach 330 ppb, the detection limit of the EPA recommended semi-
volatile analytical procedure.
5.5 Summary and Conclusions
The pilot scale treatment facility demonstrated under field conditions that a solid-
phase treatment process could be used to successfully treat the organic constituents
present in Pit O soil. The process removed the volatile organic compounds by air
stripping, and destroyed semi-volatile organic compounds by biodegradation. More
than 99% of the volatile organic compounds were removed within the first 21 days
of operation of the pilot test. However, the biodegradation of the semi-volatile
organic constituents was much slower. For example, it was estimated that approxi-
mately 131 days would be required to reduce the phenanthrene concentration to
non-detectable levels in the treatment facility.
861014/1-FINAL REPORT 5-18
-------
TABLE 5-15
Phenanthrene Half-Life Values in
Treatment Lanes
Lane
Control
Nutrient
Adjusted
Initial Concentration3
(nob)
27,850
19,400
Final Concentration1*
5,725
2,712
Half Life Value
(DavO
40.8
33.0
Inoculated
Multiple
Inoculated
73,600
24,360
5,750
5,275
25.7
43.3
All Data
36,302
4,866
33.0
a - Day 0
b - Day 94
861014/1-FINAL REPORT
5-19
-------
Given:
Given:
Given:
1.
2.
TABLE 5-16
Calculation of Phenanthrene Half-Life Value
Initial average phenanthrene concentration - 36,302 ppb ± 43,018, n - 16
Final average phenanthrene concentration 4,866 ppb + 2,227, n - 16
In cp. - kt
In 36.302 - k.94
4,866
k - 2.01
94
Rate constant
0.021
0.693 - k.t1/2
tl/2 " ^1
Half Life - 33.0 davs
Where co - initial concentration
c - final concentration
t - 94 days
k - rate constant
Where \.\/2m half-life
861014/1-FINAL REPORT
5-20
-------
TABLE 5-17
Effect of Initial Concentration on Phenanthrene Degradation
Initial Concentration (cob) Average Reduction
1,000 - 4,999 27.4
5,000 - 9,999 33.4
10,000 - 49,999 67.2
50,000 - 100,000 94.0
> 100,000 96.7
861014/1-FINAL REPORT 5-21
-------
FIGURE 5-1
Reduction in Phenanthrene Concentration
in Pilot Test Area
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861014/1-FINAL REPORT
5-22
-------
Chapter 6
FULL-SCALE TREATMENT FACILITY
-------
6. FULL-SCALE TREATMENT FACILITY
The pilot-scale treatment facility installed at the Brio/DOP site effectively demonstrated
an efficient, cost-effective process for remediating organic compounds found in affected
materials and soils. This process removed volatile organic compounds from affected
materials and soils by air stripping and destroyed semi-volatile organic compounds by
biodegradation. Air stripping volatile organic compounds and trapping of the vapors on
activated carbon was a fast and effective process, removing more than 99 percent of the
volatile organics in the first 21 days of operation. Biodegradation of the semi-volatile
organic compounds was a much slower process, requiring over 100 days to reduce
phenanthrene concentrations below detection limits.
Scaling up the pilot-scale solid-phase biodegradation facility evaluated at the Brio/DOP
site can be accomplished by simply increasing the size or number of treatment beds.
With plastic-film greenhouse components readily available, the full-scale facility would
most likely consist of a series of long side-by-side greenhouses. Although such a facility
'would be effective in reducing volatile and semi-volatile organic compound concentra-
tions, the total time required to treat affected materials and soils might be unacceptably
long. The only way to substantially decrease the treatment period is to use an aqueous-
phase biodegradation process.
6.1 Feasibility of Aqueous-Phase Biodeeradation
A biological treatment process with the potential for removing and degrading the
organic compounds found at the Brio/DOP site is aqueous phase biodegradation.
Degradation of organic compounds is more rapid when the microorganisms and target
compounds are in an aqueous solution. The aqueous solution greatly improves
contact between the microorganisms and their required nutrients and oxygen as
compared to the solid-phase process. The increased contact can increase the
biodegradation rate by as much as an order of magnitude. For example, half-life
values for phenanthrene in the on-site aqueous phase fermentation vessel were 1.4
to 2 days (Table 6-1). These half-lives are significantly less than the 33-day half-
life value for phenanthrene in the solid-phase pilot-scale biodegradation system.
Furthermore, in-house research and development by Ecova Corporation has demon-
strated that in aqueous phase systems half-life values for a number of polynuclear
aromatic hydrocarbons are commonly in the 3 to 4 day range (Table 6-2).
Given this information, aqueous biodegradation is a feasible process for rapid
degradation of the semi-volatile organic compounds found at the Brio/DOP site.
6.2 Full-Scale Aoueoua-Phaae Biodearadation Facility
The full-scale aqueous phase system would consist of four major components (Figure
6-1):
o Materials handling and slurry mixing
o Biodegradation/Air Stripping
o Dewatering and disposal
o Process support and monitoring
861014/1-FINAL REPORT 6-1
-------
TABLE 6-1
Half-Life Values for Phenanthrene
in the Fermentation Vessel
Run
02
Initial
Concentration
(ppb)
187
190
Final
Concentration
(ppb)
16a
9b
Half-Life
Value
(days)
2.0
1.4
a After 8 days' incubation at ambient temperature
b After 15 days' incubation at ambient temperature
TABLE 6-2
Half-Life Values for Polynuclear Aromatic
Hydrocarbons in Bench-Scale Aqueous Phase Systems
Compound
Phenanthrene
Naphthalene
2-MethyI naphthalene
Fluorene
Fluoranthene
Acenaphthene
Initial
Concentration
(ppb)
180,000
250,000
: 81,000
59.000
86,000
73,000
Final
Concentration*
(ppb)
BDL (660)b
BDL (660)
BDL (660)
BDL (660)
BDL (660)
BDL (660)
Half-Life
Value
(days)
3.5
3.3
4.1
4.3
4.1
4.1
a After 28 days incubation on a rotary shaker at 25°C
b BDL - Below Detection Limit (Estimated Detection Limit)
Source: Ecova Corporation, Unpublished Information
861014/1-FINAL REPORT
6-2
-------
MECHANICAL EQUIPMENT
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Process ind Instrumenlalion
Aqueous-Phase System
WCIKAl fTTO PUUT
CCMEBESSeaS
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[ t t VJ>
86IOI4/1-FINAL REPORT
6-3
-------
6.2.1 Materials Handling and Slurry Mixing. Two materials handling alterna-
tives have been identified: 1) excavate soils and transport to a central
facility where they are mixed with water and subsequently remediated, or
2) mix the soil slurry at the pit and pump the soil slurry to the treat-
ment facility.
6.2.2 Biodegradation and Air Stripping. Microbiological treatment of the soil
slurry would be accomplished in either a series of aboveground carbon
steel bioreactor tanks or in a series of enclosed lined ponds. Either of
these options would accommodate an efficient continuous process.
Aeration and agitation, which are required to optimize the microbiological
process, would be supplied by compressed air sparging and/or mechanical
mixing. Adjustment of pH and the addition of microbial inocula, if
required, would be accommodated by the process design.
If the concentrations of organic compounds in the incoming slurry
exceeded some predetermined, biologically limiting level, clean effluent
slurry could be recycled for dilution.
All volatile organic compounds would be contained and recycled through
the slurry using the air compression and sparging system. Any air
discharged to the atmosphere would pass through vapor phase carbon
units.
6.2.3 Dewatering and Disposal. Dewatering would be accomplished through
thickening in a clarifier followed by a filtration or drying process. The
dewatered soils would be hauled to the excavated pit area and used as
backfill. Effluent from the filtration/drying process would be recycled
back to the slurry mixing location.
6.2.4 Process Support and Monitoring. Process support facilities would include:
o Office, control room, and laboratory facilities
o Equipment and material storage areas
o Equipment maintenance area
o Stand-by emergency power operation
6.3 Summary and Conclusions
The pilot-scale treatment facility effectively demonstrated an efficient, cost-
effective process for remediating the organic compounds found in Pit O soil. The
process removed volatile organic compounds by air stripping and destroyed semi-
volatile organic compounds by biodegradation. Although such a facility would be
effective in reducing the concentrations of volatile and semi-volatile organic
compounds, the time required to treat affected materials and soils might be unac-
ceptabiy long. An aqueous phase biodegradation process would increase the rate of
removal of organic compounds. Ecovt Corporation therefore concludes that an
aqueous phase biodegradation process is the optimum system for removing the
organic compounds in the required period of time, assuming that site remediation
must be accomplished in less than five years.
861014/1-FINAL REPORT 6-4
-------
Chapter 7
CONCLUSIONS
-------
7. CONCLUSIONS
A solid-phase treatment process can be used for removing or destroying the organic
compounds detected in Pit O soil.
The process removes volatile organic compounds by air stripping, and destroys semi-
volatile organic compounds by biodegradation.
Although such a facility would be effective in reducing the concentrations of
volatile and semi-volatile organic compounds, the time required to treat affected
materials and soils by a solid-phase treatment process might be unacceptably long.
An aqueous phase biodegradation process would increase the rate of removal of
organic compounds. Ecova Corporation therefore concludes that an aqueous phase
biodegradation process is the optimum system for removing the organic compounds
in the required period of time, assuming that site remediation must be accomplished
in less than five years.
861014/1-FINAL REPORT 7-1
-------
Chapter 8
REFERENCES CITED
-------
8. REFERENCES CITED
Bouwer, E.J., and McCarty, P.L. (1983). Transformations of 1- and 2-carbon halogenated
aliphatic organic compounds under methanogenic conditions. Appl. Environ. Micro-
biol., iL 1286-1294.
Bouwer, E.J., and McCarty, P.L. (1985). Utilization of Trace Halogenated Organic
Compounds in Acetate-Grown Biofilms. Biotechnology and Bioengineering, 27, 1564-
1571.
Bushnell, L.D. and Haas, H.F. (1941). The utilization of certain hydrocarbons by micro-
organisms. Journal of Bacteriology, 41, 653-673.
Cerniglia, C.E. (1984). Microbial Transformation of Aromatic Hydrocarbons. In Petroleum
Microbiology, Atlas, R.M. (ed). MacMillan Publishing Company, pp. 99-128.
Coover, M.P., and Sims, R.C. (1987). The effect of temperature on polycyclic aromatic
hydrocarbon persistence in an unacclimated agricultural soil. Hazardous Waste and
Hazardous Materials, ±, 69-82.
Dalton, H., Prior, S.D., Leak, D.J. and Stanley, S.H. (1984). Regulation and control of
methane monooxygenase. In Microbial Growth on C-l Compounds. Crawford and
Hanson (eds). pgs. 75-82.
Fogel, MM., Taddeo, A.R., and Fogel, S. (1986). Biodegradation of chlorinated ethenes
by a methane-utilizing mixed culture. 1986. Appl. Environ. Microbiol. 51. 720-724.
Gossett, J.M (1985). Anaerobic degradation of Cj and C2 chlorinated hydrocarbons.
AFESC Final Report AD-A165 005. 153 pgs.
Jhaveri, V., and Mazzacca, A.J. (1983). Bioremediation of ground and groundwater by in-
situ biodegradation, case history. In Proceedings of the 4th National Conference on
Management of Uncontrolled Waste Site, October 31 to November 2, Washington,
D.C.
Jhaveri, V., and Mazzacca, A.J. (1985). Bioreclamation of ground and groundwater case
history. In Proceedings of the 6th National Conference on Management of Uncon-
trolled Hazardous, November 4 to 6, Washington, D.C.
Kaufman, D.D. (1983). Fate of toxic organic compounds in land-applied wastes. In J.F.
Parr, P.B. March and J.M. Kla (eds.) Land Treatment of Hazardous Wastes. Noyes
Data Corp. Park Ridge, NJ. pgs. 77-151.
Kleopfer, K..P., Easly. D.E., Haas, B.B., Jr., and Delhi, T.G~ (1985). Anaerobic degradation
of trichloroethylene in soil. Environ. Sci. Technol.. lg. 277-280.
Loehr, R.C. and Malina, J.F. (eds). (1986). Land Treatment: A Hazardous Waste
Management Alternative.
Nelson, M.J.K., Montgomery, S.O., O'Neill, E.J., and Pritchard. P.H. (1986). Aerobic
metabolism of trichloroethylene by a bacterial isolate. Appl. Environ. Microbiol. 52.
383-384.
861014/1 8-1
-------
Nelson, M.J.K., Montgomery, S.O., Mahaffey, W.R. and Pritchard, P.H. (1987). Biodegrada-
tion of Trichloroethylene and Involvement of an Aromatic Biodegradative Pathway.
Appl. Environ. Microbiol., 5J, 949-954.
Ovcrcash, R., and Pal, D. (1979). Design of Land Treatment Systems for Industrial
Wastes-Theory and practice. Ann Arbor Science Publ. Inc. Ann Arbor, MI.
Parr, J.F., Sikora, L.J., and Burge, W.D. (1983). Land Treatment of Hazardous Wastes.
Noyes Data Corp. Park Ridge, N.J.
Parsons, F., Wood, P.R., and DeMarco, J. (1984). Transformations of tetrachloroethene
and trichloroethene in microcosms and groundwater. J. Am. Water Works Assoc.
26:56-59.
Patel, R.H., and Hou, C.T. (1984). Enzymatic transformation of hydrocarbons by mcthano-
trophic organisms. Dev. Ind. Microbiol., 22. 141-163.
Perry, J.J. (1979). Microbial Co-oxidations Involving Hydrocarbons. Microbiological
Reviews, 43_ 59-72.
Ryan, J. (1986). The land treatability of Appendix VIII organics presented in petroleum
industry wastes. In Land Treatment: A Hazardous Waste Management Alternative.
Water Resources Symposium No. 13. TX. Center for Research in Water Resources.
The University of Texas, Austin, TX. Pgs. 347-367.
Sielicki. M, Focht, D.D. and Martin, J.P. (1978). Microbial transformations of styrene and
[14C] styrene in soil and enrichment cultures. Appl. Environ. Microbiol., 3JL 124-128.
Sims, R.C. (1986). Loading rates and frequencies ,for land treatment systems. In. Land
Treatment: A Hazardous Waste Management Alternative. Water Resources Symposium
No. 13. Center for Research in Water Resources. The University of Texas, Austin,
TX. pgs. 151-170.
Singleton, P. and Sainsbury, D. (eds). (1978) Dictionary of Microbiology, 1978. J. Wiley
and Sons Ltd. (pub).
Tabak, H.H., Quave, S.A., Mashai, C.I. and Barth, E.F. (1981). Biodegradability studies
with organic priority pollutant compounds. J. Water Pollut. Control Fed. 5J.: 1,503-
1,518.
Vogel, T.M and McCarty, P.L. (1985). Biotransformation of tetrachloroethylene to
trichloroethylene, dichloroethylene, vinyl chloride, and carbon dioxide under
methanogenic conditions. Appl. Environ. Microbiol., 42, 1080-1083.
Wilson, J.T. and Wilson, B.H. (1985). Biotransformation of trichloroethylene in soil. Appl.
Environ. Microbiol., 12, 242-243.
861014/1 8-2
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APPENDIX A
Glossary
-------
Aerobe
Aerobic
Anaerobe
Anaerobic
Bacteria
(singular: bacterium)
Broth
Carbon and
energy source
Cell
Colony
Culture
Enrichment culture
Enzymes
APPENDIX A
Glossary
Any organism which grows in the presence of air or
oxygen.
Refers to an environment in which the partial pressure of
oxygen is similar to that which occurs under normal
atmospheric conditions.
Any organism which grows in the absence of air or
oxygen.
Refers to an environment in which no oxygen is present.
A group of diverse and ubiquitous prokaryotic single-
celled organisms.
A term used in bacteriology to refer to any of a variety
of liquid media.
A substrate that provides an organism with carbon and
energy.
Basic structural and functional unit of all living organisms
except viruses.
Collectively, a number of individual cells or organisms of
a given species which, during their development, have
formed a discrete aggregate or group.
A growth of particular type(s) of microorganism on or
within a solid medium, or in a liquid medium, formed as a
result of the prior inoculation and incubation of that
medium. Mixed culture: one containing two or more
species or strains of an organism. Pure culture: one
comprising organisms which ire all of the same species or
strain.
Any form of culture, in a liquid medium, which results in
an increase in the numbers of a given type of organism
relative to the numbers of other types of organism which
may be present in the inoculum: the enrichment medium
may contain substance(s) which encourage the growth of
the required organism or which inhibit the growth of
other types of organism.
Proteins which function as highly efficient biological
catalysts. An enzyme increases the rate of a (thermo-
dynamically feasible) reaction without altering the
equilibrium constant for that reaction; reactions which, in
the absence of an enzyme, require extreme conditions of
861014/1-FINAL REPORT
A-l
-------
Eukaryote
Fungi
(singular: fungus)
Heterotroph
Incubation
Indigenous
Inoculate
Inoculum
(plural: inocula)
Medium
(plural: media)
Metabolism
Methanogcn
Microflora
Microorganism
Morphology
Physiology
Prokaryote
Substrate
Yeasts
temperature, pH, etc., can occur rapidly under physio-
logical conditions in the presence of an appropriate
enzyme.
An organism made up of cells with true nuclei e.g.
algae, fungi, protozoa and yeasts.
A group of diverse and widespread unicellular and
multicellular eukaryotic microorganisms.
Any organism which requires a range of exogenous organic
compounds for growth and reproduction.
The maintenance of a particular ambient temperature for
inoculated media.
Originating within.
To introduce a microorganism into a suitable situation for
growth.
The material used to inoculate a medium: an inoculum
typically comprises or contains viable microorganisms.
In general, any material which supports the growth/
replication of microorganisms.
The chemical and physical processes continuously going on
in living organisms and cells.
A methane-producing bacterium.
The totality of microorganisms associated with a given
environment or location.
The term microorganism commonly includes the following
types of organism: algae, bacteria, blue-green algae, fungi,
lichens, protozoa, yeasts and viruses.
Form and structure of an organism.
The functions and vital processes of a living organism.
An organism lacking a true nucleus in the cell - e.g.
bacteria.
The substance that is used by a microorganism for growth.
A category of fungi defined in terms of morphological and
physiological criteria.
861014/1-FINAL REPORT
A-2
-------
APPENDIX B
Analytical Chemistry Methods
-------
APPENDIX B
Analytical Chemistry Methods
B.I GC/MS Analyses
Volatile organic and base/neutral/acid compounds were analyzed by GC/MS utilizing
U.S. EPA methodologies 8240 and 8270, respectively. Due to the ppm concentration
of organic compounds in the samples, which extended beyond the linear calibration
range of the instrumentation, additional sample preparation methods were necessary.
The methods used were from the U.S. EPA Contract Laboratory Program protocols
for handling samples of wide ranging concentrations in order to achieve quantifiable
results for higher concentration constituents. Utilizing these methodologies, samples
were prepared as low level (lower detection limits) or medium level (medium
detection limits) extractions. Following the EPA protocols, results were reported on
the method achieving quantifiable results for the highest level constituents. Results
were adjusted when possible for dry weight.
With these methods, detection limits varied significantly, depending on the amount
of sample initially prepared or dilutions performed. Because some of the analytes of
interest were below the detectable limits (BDL), results for all the constituents
potentially present in the samples were not obtained. However, baseline levels for
all major constituents were measured. A summary of methods used for each
analysis follows.
Analysis Method
VOC Low Level Soil Method. Five (5) grams of soil were weighed
into a purge and trap vessel and 5 milliliters (ml) of organic
free water was added to the vessel. The vessel was attached
to a Tekmar LSC-2 purge and trap device interfaced to a
GC/MS via a heated transfer line. The sample was then
sparged and analyzed according to Method 8240 of SW 846.
Medium Level Soil Method. Four (4) grams of soil were
weighed into a VOA bottle, to which 100 ml of methanol was
added. The bottle was capped and shaken. One hundred
microliters of the methanol extract was taken and added to 5
ml of organic free water. This mixture was then introduced to
the Tekmar purge and trap device and analyzed according to
Method 8240 of SW 846. If constituent levels exceeded the
linear range of calibration, a smaller aliquot of the methanol
extract was taken and analyzed.
The GC/MS system was calibrated by performing a 5-point
calibration curve. The instrument was tuned daily to meet
mass spectral criteria. After meeting tune, a daily standard
was analyzed and response factors were verified to the
multipoint calibration response factor. Following the standard,
a water blank was analyzed to verify reagent water purity and
ensure that the instrument was free from compounds interfering
with the analysis. Samples were then analyzed.
861014/l-FINAL REPORT B-l
-------
Base/Neutral/Acid ^ow Level Soil Method. A thirty (30) gram portion of soil was
extracted three times with methylene chloride/ acetone mixture
by sonication. The extracts were filtered and combined. The
combined extracts were reduced to 1 ml extract volume and
analyzed by GC/MS Method 8270 of SW 846.
Medium Level Soil Method. One (1) gram of soil was used in
lieu of 30 grams and was extracted and analyzed as the low
level soil method for base/neutral/acids. If concentrations
were greater than the linear range of calibration curve the
analysis was repeated with a dilution of the extract.
The GC/MS system was calibrated by performing a 5-point
calibration curve. The instrument was tuned daily to meet
mass spectra criteria. After meeting tune a daily standard was
analyzed and response factors were verified to the multipoint
calibration. Samples were then analyzed.
Because of the high water content of the enrichment culture samples, some modifi-
cations to the standard sample preparation procedures were applied. Results are
based on wet weight. The modifications summary is as follows:
Analysis Method Modification
VOC Samples for the volatile organic analyses were collected from
the enrichment culture flasks by hand shaking the flask to
create a homogenous suspension. While in suspension, 10
milliliters were poured into a culture tube. The tube was
transferred to the GC/MS laboratory for analysis.
Prior to analysis, the culture tube was shaken again to produce
a suspension and 5 ml aliquot was removed from purge and trap
analysis by GC/MS.
Base/Neutral/Acid The remaining portion of the enrichment culture, after taking
an aliquot for VOC analysis, was transferred to a centrifuge
bottle. The water was separated from the soils by centrifuging
and water was decanted. The remaining soil was then ex-
tracted by the soil methods and the extract reduced to a 1-ml
volume for analysis by GC/Ms, and corrected for weight.
Because of the high water content of the fermentation vessel samples, the standard
sample preparation methods were modified as follows:
Volatile Compounds Samples for volatile organic analysis were received in 40 ml
VOA vials from the field. Prior to analysis, the vial was
shaken to produce a homogeneous suspension. A 5-ml aliquot
of the suspension was then removed for purge and trap
analysis by GC/MS. Calculations were performed on a slurry
volume basis.
861014/l-FINAL REPORT B-2
-------
Base, neutral, and Approximately one liter of sample was centrifuged to separate
acid-extractable the liquid and solids. The liquid was decanted and the volume
compounds recorded. The entire volume of liquid was then extracted by
EPA procedure 625. Thirty grams of the remaining solids were
then extracted by EPA procedure 8270 of SW846. Prior to final
concentration, the liquid and solid extracts were combined.
The extract was then reduced to 1 ml and analyzed by GC/MS.
Calculations were performed on a slurry volume basis.
B.2 Nutrient and oH Analyses
Analysis of soluble ammonium, nitrate, phosphorous and pH was performed on a
water extract of the soils. The extraction was performed by taking 100 grams of
each soil mixed with 500 ml of water. The mixture was allowed to stand a minimum
of 12 hours. The water extract was filtered to remove particulates and analysis was
performed on the extract. A summary of methods for each analysis follows.
Analysis
Ammonia (as N)
Nitrate (as N)
Phosphorous (as
PH
B.3 Other Analyses
Method
Standard Method 417E (Ammonia Selected Electrode Method).
The ammonia analyses were performed using an Orion model
9512 specific ion electrode in conjunction with an Orion model
940 Ion Analyzer. The concentration was read directly in mg/1.
Nitrate was analyzed using a modification of standard method
418.C (Cadmium Reduction Method). The modification utilized a
Hack Cadmium Reduction Method using Nitrayer 5 Nitrate
Reagent. With this method, the solution develops an amber
color proportional to the concentrations of nitrates in the
water and the concentration of nitrate is determined color-
metrically.
Standard Method 424E (Stannous Chloride Method). Phosphor-
ous was determined colormetrically by converting all phosphor-
ous forms to molyblophosphoric acid. This was reduced by
stannis chloride to intensely colored molybdenum blue, which
was read colormetrically on a Milton Ray Spec 20 with a 5
point calibration curve.
EPA Method 150.1. With this method, the water extract from
soil was measured by a Ross 8104 combination pH Electrode and
read directly on an Orion 940.
Moisture Content
Metals
Ten grams of soil were dried to 105°F for a minimum of
24 hrs.
ICP Metal Method Digestion was by Method 3050 from
SW 846, Acid Digestion of Sludges. Analysis was by
Method 6010 from SW 846, Inductively Coupled Plasma.
861014/1-FINAL REPORT
B-3
-------
Total Organic Carbon EPA Method 415.1 Organic carbon in a sample was
converted to C02 by catalytic combustion. The C(>2
formed was measured directly by an infrared detector.
The amount of C(>2 was directly proportional to the
concentration of carbonaceous material in the sample.
861014/1-FINAL REPORT B-4
-------
APPENDIX C
Analytical Chemistry Data:
Pit O Characterization
-------
-------
APPENDIX C
Analytical Chemistry Data:
Pit O Baseline Characterization
861014/1-FINAL REPORT C-l
-------
TABLE C-I
Pft O Nutrient Analysis
Baseline Characterization
Sampling Location
SAMPLE 1
AMonia (
Phosphate
Potass iu«
Nitrates
P«
g NH3-N/kg)
(*g P04/kg)
(g K/kg)
(g MOS-N/kg)
CH0132
2.5
1.3
3.3
9.3
6.7
CH0133
0.6
1.3
3.2
4.3
7.1
CHOI 34
0.6
1.3
3.3
2.5
7.4
CHOI 35
6.0
1.3
4.0
1.3
7.6
CH0136
0.7
1.3
3.3
6.5
7.1
CH0137
0.9
1.4
3.4
4.6
7.1
' CHOI 39
142
1.4
23
9.9
1.1
CH0140
101
1.3
13
4.3
1.9
CH0141
135
1.3
21
S.6
7.9
' CH0138
1.1
1.4
5.5
6.3
1.1
t Moisture 24.39 22.41 24.21 26.22 24.25 26.51 21.49 25.13 23.76 27.97
Total Organic
Carbon 1*9/kg) 10000 4000 6900 12000 4900 1700 15000 14000 12000 33000
Nott: AMonia, phosphate, nitrate, and potasslu* analyses
wt pcrforatd on a 1:5 soil to water extract vith
final quantltatlon based on dry weight of soil.
861014/1-FINAL REPORT C-2
-------
TABLE C-2
Pit O Metal Analysis
Baseline Characterization
(Concentration in rag/kg)
a
b
M
e
N
S
z
*
9
U
b
u
o
u
- o
k
II
M B
f.
e
e
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w
«0
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O
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ggggggggg
n««i«r'*O«« *;
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§ i i i i i i i i §
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olooiooioi
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s
861014/1-FINAL REPORT
C-3
-------
APPENDIX D
Analytical Chemistry Data:
Bench-Scale Biodegradation Evaluation
-------
APPENDIX D
Analytical Chemistry Data:
Bench-Scale Biodegradation Evaluation
861014/1-FINAL REPORT D-l
-------
TABLE D-l
Enrichment Culture Chemistries
Sample CH0132
Concentrations in ng/g (ppb)
CHTUIIUTS
snorts
ICItM
i-tut Mian*
IXttal
toe
HI 111
HL(IIM) ML(SI)
171
111
Ml
141
IM
nueriw
IM
III
It.l
MM1-OUIII CNlttlUTED
Kmouwats
Chlmfm
ChteM*
1 . 1 .M.
Ml IOL(K)
«tr 11
14170 til
nun i«ii(t)
mi sn
in
i»i
Sill OOL(7S)
11111(1)
1IIS
III
1111 OK.(11I)
001(111)
OOL(«) 17
11
111
111
KM »)
KM")
11M
«.(}$)
71
001(7$)
III
ML(H)
11.1
11.7
11.1
IM
11.1
11.7
11.1
auttura uoutic
MM til
IH1 ML(lll)
till c
IIM M.(IM)
1111
tin MM
US
111 OOL(») 001(11) M.I
001(111)
tin IIH
M.(11I) ML(1M)
uouTies
<#^^^Ad
K^^WW
KM
111
117
O7
I1M SIM
t(MI) OOKIM) OOL(III)
471
MUD OK.(IM)
MUD ooi(iM)
OOL(71II) OBL(IMI)
OOL(UO) Mt(IM)
L(ISM) «M7M
OOL(IM)
OOL(H) M.(IS)
001(111)
IIM mo
0».(11I)
OOL(11»)
L(HM)
Hl.l
U.I
TO: (*) Tklt
(*) llw
(e)
klw*.
Note: 28 dav rest period
8610U/1-FINAL REPORT
Ocun^ IM Mix tta «tM wMntlMativi V
cwCMlMttan «t(i) ttit, ttuetlm Itoit (bttattW 0>««etl«< t««(
D-2
I MlflK ( Mil
-------
TABLE D-2
Enrichment Culture Chemistries
Sample CH0133
Concentrations in ng/g (ppb)
CONTUtlJUITS
IETOKES
tenant
t-fctlMM
u
111
1111
WEI HOUCTIOK
»l 111 lit
BX.($0) «.( 10) W.0»)
SNMT-OUII CHIOI1IUTED
NYMOUUONS
IKthylm* Chlorite
I.I.MMcMoraithiiM
l.l.lrTrlditoottlMM
1211 I1(k)
Hit 111
U2II »S!S(c)
MM
HL(tlOO)
SI II 1} 11.3
1MI(e) 111 1< ll.i
HIT(e) MST(e) !»() K.I
Ml(HI) ML(KI) W.(1SI) >IM
Mc(ni) ML(iii) ioc(no)
W.(2S) KX(J) Hl(S)
CNUKIUTEO UOMTIC
HYOMOUtlOKS
L(UM) M.(IS) IK 11
IN ML(IM) HL(1M) M.(1II)
1SII ML(IM) ML(1it) tOUnt) UUnt)
ttt ML(IM) n.(1H) Kill) HKISI)
W.(IM) ML(iii) taunt) w.(iu)
IN M.(1U)
UDMTICS
T«al
1MI
ML(1M) HL(III) M.(UI)
12M II*
TIS: () IMt iMkw Utan 1rm
(k) MM fMttf ta klwk
HNtwlMt1*i
(c)
tatNtri kut talM tta
Italt
lictt)
thrry.
Note: 28 day test period.
S61014/1-FINAL REPORT
D-3
-------
TABLE D-3
Enrichment Culture Chemistries
Sample CH0135
Concentrations in ng/g (ppb)
\ m
CMC BU W Wn WCl KCUCTION
CMTMUUITS
mows
tenant ni(Ult) SI ML(tt) *
111} Hl(i) Mt(1l) HL(II) NR.(10) >M.J
SKMT-CKIIK CXlttlMTCD
HTDMCitlONS
CMorofora 111 m.(f) M.(S) HL($) HL(S) >I7.I
MtM*"* Chlwid* IMS II II 11 « II. I
l.l.MrtcMorottHiftt «.(»«> II II II
l.l.I.I.-TttrtcMaratttiMM Ml(tll) I II t II
NMMhlwefeutWttn* IOL(IU) IM.(IM) Ml(IU)
1.1.1-TrtchlorattttiM m(tlt) HL(S) II ML(S) «.($)
WLOHHTEO MOUTIC
HYMOCilHNS
Ch1«rob«nx«* «.(!») 11 SI tt 11
HL(IN) HL(IH) M.(1tt)
III W.(IM) «L(WI) M.(ni)
* W.(IM) W.((M) Hl(fH)
1.2.1-TrteMorokMitM ML(IM) *
M«uehlor«bwi*M 111 * « ITI SIN
UN W.(1IN) M.(ltM) HL(TII)
UOMTICS
Tettl XylMt 111 ML(S) NC(S) HL(I) M.(S) >«.!
Ettyltantm HI «.({) HL(|) «.($) Bl(l) >II.S
tiMMttrtm 1I2T W.((U) M.(W) NL(ttt) M.I
totta-Mm NC(II$) TN HMNI) «.(««) «.(«)
kntele teM W.(I1ii) W.(MN) MINI)
MTB: () Ihta
(») tin fouW ta kin*. *an1kU/tntekU
f tMf)*
' (e) MM* *]HMt1t«t1«n rinf*
tet«et«4 tot t»t«» tta MtM «nM«f kjtto Itoit
W.(i) Ittaf iKtcttai Itett (Ut««ft«< Maettan
MtUtf U rfW
-------
TABLE D-4
Enrichment Culture Chemistries
Sample CH0136
Concentrations in ng/g (ppb)
CONTAMINANTS
KETONES
Acetooi
2-Buttnont
Initial
Cone MA
BHPY
WEI
\ AVE
REDUCTION
37 13 101(10) BOL(IO) 801(10)
3(b) 19 810(5) 801(10) BDL(IO)
23.7
SHORT-CHAIN CHLORINATED
HYDROCARBONS
Methylent Chloride
1,2-Olehlorotthane
1,1,2-Tr1chloroith«n«
CHLORINATED AROMATIC
HYDROCARBONS
Chlorobenzene
Huciehl orobanzani
AfiOMATICS
Ethylbtnzana
4 11
37 801(5)
122 IOL(S)
15
80L(S)
HI
80L(S)
BOL(S)
2 101(5) 101(5)
* 601(740) BOL(TtO)
II
BOL(S)
OL(S)
10 IOL(S)
* BOL(7SO)
80L(S) 101(5) BOL(S) ttL(5)
IS.1
>!S.J
NOTES: (a) * This nuabar takw froa a dilution
(b) Also found 1n blank, osslbla/probabla eontaalnatlon
of saapla
(e) Abova quantltatlon ranga
* Oataetad but balo* tha aathod «u«nt1fieat1on Halt
MM.(x) Ba1o« Oataetlon L1a1t (Estlaatad Oatactlon Halt)
I Volatlla organtp data Is raportad par oraa of soil
slurry, lasa. nautral, acid data 1s raportad par
fraa Mt Might of Mil
Note. 28 day test period.
861014/1-FINAL REPORT
D-5
-------
TABLE D-5
Enrichment Culture Chemistries
Sample CH-0139
Concentrations in ng/g (ppb)
ornes
letter*
2-Munont
Cone
X(ISOO) II
IX(«00) IOC(U)
Ml
» m
KHCTIOI
II
101(11) ML(1I)
SHMT-OUIII
MthyUn* Chlerldi Jilt
1.1.I-Ti-1eK1or«thM< KX.(1100)
Trteklaroithm Hl(llll)
I.I.MrtehlorMthiM ML(t)M)
I 11 II 12
* HL(S) HL(f) «.($)
HL(S) «.($) HL(I)
«L($) HL(S) I
B.I
onoiiuni UOMTIC
101(1111) HL(I)
L(t)
UMAT1CS
t«u» Iy1«iM
Etkyltantw*
Styrm
ITT
till
Hl(llN)
«-(4SI)
HL(tlM)
M.(S) HL(S)
«L(I) HMD MUS) HL(S)
HUD «L(I) M.(S)
HL(TTI) «.(«!) HL(TM)
>M.J
Ml.l
WTCS: (a) * Tk1«
(k) IlM fwrf to klwk. NntkWr^kU
f
(c) MOM quMClUtfan
tot^t* kut talw thi MtM qvMKlftattai Itolt
L(i) §! tKWtta Ltalt (Efttattlri IttMttai IMt)
I V*l*t1U trfMle 4tt» H i»r>i< fir frw if toil
I«M. MWtrtl,
Note: 28 day test period.
S61014/1-FINAL REPORT
D-6
-------
TABLE D-6
Enrichment Culture Chemistries
Sample CHOI40
Concentrations in ng/g (ppb)
CONTAMINANTS
KETONES
Acetone
2-Botinoni
Initial
Cone BHA
* AVE
8H 8HPY BHEI REDUCTION
9 34 BOL(IO) * BOL(IO)
2(b) 14 801(10) BOL(IO) IOL(10)
SHORT-CHAIN CHLORINATED
HYDROCARBONS
Mathyleni Chloride
208 STI(a)
NOTES: (a) * This mmber taken free a dilution
(b) Also found in blank, tesible/praaabla contamination
of ««ple
(e) Above quantitation range
* Detected but below the s*thod quantification Halt
MH.(x) Below Detection Llalt (Estimated Osttetian L1t1t)
I * Volatile organic data 1s reported par graa af sail
slurry, tote, neutral, acid data 1s reported par
graa net weight of soil
Note: 28 day test period.
861014/l-FINAL REPORT
D-7
-------
APPENDIX E
Pilot-Scale Treatment
Facility Construction and Operation
-------
APPENDIX E
Pilot-Scale Treatment Facility and Construction Operation
E.I Facility Construction
The field pilot test facility was designed to simulate system characteristics and
operating conditions of a full-scale solid-phase soil treatment operation. The pilot
test facility included a containment enclosure, a field office, microbiological support
system, and an emission control system (Figures £-1, E-2, and E-3). The contain-
ment enclosure was designed to contain volatile organic vapors by totally enclosing
the treatment area.
The treatment area was constructed with a liner, leachate collection piping system,
and sump to prevent leachate escape.
Organic vapors were treated by the emission control system, and leachate was
treated in the microbiologic support system.
a. Treatment Area Preparation. Following surveying, preparation of the soil
treatment area consisted of clearing, scarifying, and recompacting the upper 6
in. of soil. Fill was placed to provide a structural base for the liner and
leachate collection system. Berms and drainage swales were constructed as
necessary.
b. Area Staking and Bermine. Prior to receiving the soil from Pit O, locations
within the primary treatment facility were grade staked to establish limits of
site preparation earthwork, access road construction, and soil excavation. In
addition, areas were graded to establish and maintain drainage features. Berms
were graded to assist in run-off control for the treatment and soil excavation
areas.
c. Utilities Connection. Power (3-phase, 230-voIt) for the treatment facility was
extended from existing electrical sources. A telephone was installed in the
site office trailer. Portable chemical toilets were provided on-site. A
nonpotable water supply, available on-site, provided water to the treatment
facility.
d. Building Construction. The containment enclosure was an engineered, steel and
plastic, gutter-connected greenhouse. Materials were prefabricated and
delivered to the project site. The structure was erected over the treatment
area to control the emission of organic compounds during Pit O soil process-
ing.
Prior to installation, the area was surveyed to establish locations for the 4
corner anchors and 4 center anchors for the enclosure structural arches. The
additional 104 anchors were located using a string line and were driven to a
depth of 24 inches.
The 58 structural arches, which support the enclosure's plastic skin, were
assembled and set over the anchors. The arches were attached to the anchors
with self tapping screws. A center purlin was mounted down the middle of
each structure using bolts. A set of side purlins was installed along the
861014/1-FINAL REPORT E-l
-------
-5
ijj... __
Hill ij!
'mis; i
"111 'I
ff!Jif!|e
iiiflJdi
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IIJSS Ii 55 1:59=11
ffe
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imiiiiiii
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o
o
00
DE
SI
§P
O
m
-------
y
n
\
TflBATMENT FACILITY PLAN
ii PatnuvmtJJoa
IM «-kvf> PVC
t ttanf iM«Mn«( Vw tvWMMrt kMtWtnf TM toHf«4« t
1 1* *»-»»i**i«« rvc w« *»« M
t MbMM nil M tf
it O I* ««« bi
LINER INSTALLATION DETAIL
»,
:?£=
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SPRAY SYSTEM DETAIL
/^
LINER ATTACHMENT DETAILS'
MO* TO CALt
1
J
471
LEACHATE SUMP DETAIL
LINER PENETRATION DETAIL/' »
NOT 1O «CMI
FIGURE E-2
Soil Treatment System Plot Pljn
*«
«""
OESCR»IION
BRIO SITE TASK FORCE
SOIL TREATMENT SYSTEM
BRIO REFINING
SITE BIOREMEDIATION
E C 0 V A I '"" tellf. hVp0 -4-D-002
-------
SPRAY DISTRIBUTION PIPE f PVC
PERFORATED DRAIN PIPE
COMPACTED CLAY
SECTION
V:l/8*-lft.,H:l/4"-1ft.
_! *2
iJ C
-------
outside edge of each structure. The inner 58 arches were paired and con-
nected with U bolts. A center gutter was placed between the two structures
in order to join the structures and allow for run-off. Six end braces,
connected to the arches with clamps, were mounted to each end of the two
structures, allowing two roll-up doors to be installed on the southern ends of
the two structures. Concrete pads were placed at the base of the door frame
to serve as supports for the doors and to aid in the structure's "tightness". A
6 ml UV stabilized polyethylene covering was stretched over the structure in 4
pieces and attached with connectors.
e. Liner and Leachate Collection System Construction. The soil treatment system
constructed within the enclosure included a leachate collection and removal
piping system and a synthetic liner beneath the zone of soil processing and
treatment. The system included a permeable collection zone, a system of
lateral subdrains, and a gravity sump. Drain systems were designed for gravity
flow using perforated PVC pipe and the collection sump was sized for the
anticipated maximum inflow (20 gal/min) during the operation of the soil
treatment area. The synthetic liner was constructed of 80-mil high-density
polyethylene and included surrounding piping which discharges to the collection
sump to avoid compromise of liner integrity. The leachate collection piping
provided drainage to the south ends of the structures where each penetrated
the liner, was manifolded with the others, and ran into the leachate collection
sump. The sump had a volume 140 gallons. Accumulated leachate was pumped
from the collection sump to the microbiological support system for treatment.
The permeable collection zone consisted of a layer of bank sand ranging in
thickness from 6 inches at the inner edges to IS inches at the outer edges,
forming a nearly level surface on which the Pit O soil was applied. The edges
of the liner were secured to the inside of the structural arches with self
tapping screws.
f. Inoculum/Nutrient Distribution System. The overhead piping distribution
system, designed to distribute inoculum and nutrient treatments, consisted of a
transmission line manifolded to four valved distribution lines. The individual
.lines covered each of the test plots, isolating the plots for the individual
treatments by their back-to-back configuration. Thus, overspray was virtually
eliminated. The inoculum or nutrient mixtures were applied through 32
individual distribution heads per test plot. Each head was capable of supplying
0.79 gallons per minute (gpm) at 30 pounds per square inch (psi). A Holly
filter equipped with a SO mesh screen filtered the fluids before distribution.
The fluids were pumped with a positive displacement air pump.
g. Ecova Process Units. Two mobile units, each approximately 8 ft x 40 ft x 8 ft
high, were installed on-site for process support.
1. Microbiological Support System. The first mobile unit, designated the
Microbiological Support System, supported the following aspects of the
project:
o Production of microbial inocula for field applications
o Biological treatment of leachate collected during operations
o Storage of sampling and soil monitoring equipment
o Microbiological laboratory assessment
861014/1-FINAL REPORT E-S
-------
o Preservation and temporary storage of samples
o Sample preparation area
2. Emission Control System. The Emission Control System provided activated
carbon polishing of organic vapors contained by the enclosed treatment
facility. Air was removed from the treatment facility by an 8,000-cubic-
feet-per-minute (cfm) turbo fan and directed into a plenum. Air was
removed from the plenum by three individual 3,000 cfm blowers and
directed through three activated carbon absorbers. Each of the carbon
absorbers was capable of treating an air flow of 3,000 cfm. The carbon
absorbers removed volatile organic compounds and associated odors from
exhaust air. Performance of these units was monitored daily by portable
organic vapor analyzer measurements (see Section E.6 "Routine Pilot Test
Operations").
E.2 Pit O Soil Transfer
Following the construction of the treatment facility, approximately 200 cubic yards
of soil were removed from Pit O and transferred to the treatment facility in late
January, 1987.
a. Soil Excavation and Transport to Treatment Areas. Soil removal was accom-
plished using a backhoe with a reach of approximately 12 ft. Approximately
200 cubic yards of soil were removed. Soil was transported directly to the
lined soil treatment enclosure using two 100E tracked front end loaders.
b. Trench Covering. During the excavation of wastes at Pit O, care was taken to
ensure that a minimum length of trench was exposed at any one time so as to
minimize organic vapor discharge. Following excavation, the trench was
backfilled. During the interim, the area was staked and marked with hazard
tape and the trench covered with plastic.
c. Soil Processing and Spreading. The excavated soil was placed on top of the
prepared treatment bed. Due to the cohesiveness of the clay, the material was
allowed to dry on the treatment area before final grading. Final grading was
performed with the tracked front end loaders and a power rototiller attached
to a tractor. The clay was amenable to tillage after 3 days and to addition of
nutrients after 6 days. During the operation of the test, the soil was tilled
and aerated daily. Inoculum and soil amendments were applied to the pilot
test area using the overhead distribution system.
d. Air Monitoring. During Pit O trenching activity in January 1987, Ecova
monitored the air at the trench and in and around the facility enclosure.
Instruments used included:
o Flame lonization Detector (Foxboro Century OVA 128 GC)
o Photoionization Detector (Photovac TIP I)
An action level of a sustained 10 ppm above background was established during the
Remedial Investigation. If sustained levels of 10 ppm above background were
detected, readings were to be taken at the site boundary. Readings of up to 40
ppm total organic vapor were detected directly over the trench as it was being
861014/1-F1NAL REPORT E-6
-------
excavated, but diminished to background (4 ppm) at the site boundary. Readings did
not exceed 20 ppm immediately downwind of the front end loaders during soil
transfer. Inside the facility enclosure, during soil dumping and grading, measure-
ments as high as 70 ppm total organic vapor were detected. A significant portion
of this may have been attributable to the diesei exhaust vapors from the front end
loader (46 ppm at the exhaust stack head).
In order to assess personnel exposures to organic vapors, a total of 9 organic vapor
monitors (3M *3500) were worn in the breathing zones of workers engaged in the
excavation and movement of soil from Pit O to the soil treatment enclosure (Table
£1). In order to identify and quantify the specific compounds of interest, separate
monitors were exposed during two work days, January 23 and 24, 1987. Desorption
was with carbon disulfide, and analysis was conducted by capillary gas chromato-
graphy. Three compounds, 1,1,2-trichloroethane, tetrachloroethane, and 1,2-dichlor-
oethane, were identified in the air samples (see Table E-ll). Analysis showed that
in no case was the threshold limit value (TLV) of the American Conference of
Governmental Industrial Hygienists (ACCIH) exceeded.
E.3 Treatment Lane Designations
The pilot test area was divided into four lanes (Figure E-4), each of which eval-
uated a different treatment option. By varying biological operating parameters, the
optimum approach to the biodegradation process could be determined.
a. Control. The control lane was established to provide a baseline for evaluating
the effectiveness of the three treatment options. This lane received only
tilling and water addition.
b. Nutrient-Adjusted. The inorganic nutrient-adjusted lane determined the bio-
degradative abilities of the existing microorganisms within Pit O. Inorganic
nutrients (nitrogen and phosphorous) were added to the lane to stimulate the
activity of indigenous microorganisms. This treatment process assessed the
rate at which the existing microbiological population could degrade the target
organic compounds.
c. Single Inoculated. This lane was inoculated at the start of operations with
high concentrations of microorganisms isolated from Pit O (Table £-2). An
inoculum containing indigenous microorganisms was developed in the on-site
fermentation vessel and applied to the soil with inorganic nutrients. This
treatment process assessed the rate of organic compound removal achieved by
a single augmentation of the existing microbiological activity.
d. Multiple-Inoculated. This lane was inoculated at approximately ten day
intervals using inocula developed from soil removed from the lane and water
from the leachate collection system. This treatment process assessed the rate
of organic compound removal achieved by increasing the frequency of applica-
tion of microorganisms and inorganic nutrients.
861014/1-FINAL REPORT E-7
-------
TABLE E-l
Industrial Hygiene Sampling Results
Excavation of Soil From Pit O and Transport to Greenhouse
CONDUCTED JAN.23-24. 1987
SAMPLE WORKER
NUMBER NAME
TIME WEIGHTED AVERAGES («g/»3)
TIME 1,1.2-TRI TETRA- 1.2-
(MIN) CHLOROETHANE CHLOROETHANE DICHLOROETHANE
6867
9669
9617
6742
9469
9588
9385
6961
6783
9612
FIELD BLANK
R.
J.
T.
G.
R.
D.
T.
R.
J.
THETFORD
STOUT
TOWER
LAAKSO
BARLOW
CHAWES
TOWER
BARLOW
STOUT
50
525
520
520
524
510
525
645
645
645
0.0000
0.0000
0.0000
0.0000
0.0000
0.0000
0 . 0000
0.0035
0.0090
0.0017
0.0000
0.0000
0.0000
0.0000
0.0000
0.0000
0.0000
0.0000
0.0000
0.0000
0.0000
0.0000
0.0000
0.0000
0 . 0000
0.0000
0.0000
0.0007
0.0016
0.0004
APPLICABLE ACGIH TLV
45.0000
7.0000
40.0000
161014/1-FINAL REPORT
£8
-------
FIGURE E-4
Treatment Lane Designation
140'
72'
QUADRANT
NUMBER
1
N I
LANE
M
EXPLANATION,
C CONTROL
N "NUTRIENT ADJUSTED
I - INOCULATED
M - MULTIPLE INOCULATED
BRIO SITE TASK FORCE
LUL
V A
BRIO PROCESS AREA
TREATMENT
LANE DESIGNATIONS
861014/1-FINAL REPORT
E-9
-------
TABLE E-2
Enumeration of Aerobic Heterophic Microorganisms In
the On-Site Fermentation Vessel
Initial
Batch * cfu/ml soil slurry
1 2.84 x 105
2 4.73 x 106
3 3.00 x 106
4 2.17 x 107
5 2.20 x 108
6 8.40 x 104
7 1.43 x 106
NOTE: cfu colony forming units.
Incubation oeriod(davs)
8
5
4
8
4
4
4
Final
cfu/ml soil slurry
4.75 x 107
8.17 x 109
3.20 x 107
5.70 x 107
3.00 x 1012
2.50 x 10*
4.00 x 109
861014/1-FINAL REPORT
E-10
-------
E.4 Nutrient Additions to the Pilot Test Area
On January 30, 1987, approximately 225 to 250 pounds of ammonium phosphate were
added to each of the nutrient-adjusted, single-inoculated, and multiple-inoculated
treatment lanes. Ammonium phosphate was used because it provided the nitrogen
and phosphorous required by the microorganisms. The ammonium phosphate was
dissolved in site well water and applied to the required treatment lanes with the
overhead distribution system.
E.5 Operation of On-Site Fermentation Vessel
The initial inoculum applied to the single-inoculated and multiple-inoculated lanes
was composed of indigenous microorganisms collected from Pit O soil. These soil
samples were taken from the same area from which samples were removed in
December 1986. The samples were added to the 200-gallon on-site fermentation
vessel containing Bushnell-Haas medium plus 0.005% weight per volume (w/v) yeast
extract (an additional food source) to give a soil to water ratio of approximately
30% (w/v). The soil provided both the microorganisms required to degrade the
target compounds and the carbon sources necessary for the growth of these micro-
organisms. Subsequent inocula for the multiple-inoculated lane were developed from
soil removed from this lane and water from the leachate collection system.
To optimize microbial growth, air and agitation were provided to the fermentation
vessel. The number of microorganisms initially present in the fermentation vessel
was determined, and the vessel was incubated at approximately 25°C until the
number of microorganisms significantly increased (Table E-2). The inoculum was
then applied to the pilot test area with the overhead distribution system.
The concentrations of volatile and base, neutral and acid-extractable compounds in
the on-site fermentation vessel were determined on three occasions (Table E-3).
The analytical techniques employed are summarized in Appendix B.
E.6 Routine Pilot Test Operations
Throughout the pilot test a variety of environmental factors were measured to
establish background conditions. These included soil moisture content, soil pH, soil
and air temperature, enclosure humidity, and air quality both inside and outside the
treatment enclosure. Operations monitoring data are presented in the tables at the
end of this appendix.
a. Soil Conditioning. The pilot test area was tilled daily for periods ranging from
four to eight hours in order to assure maximum oxygen availability for the
microbial populations. Tilling was initiated with the control lane, progressed
to the nutrient-adjusted lane and then the inoculated lane, and ended with the
multiple-inoculated lane. After each tilling operation, the tractor-tiller was
decontaminated. This progression and decontamination precluded cross-
contamination. The soil was tilled to a depth of 6 to 8 inches. Accumulation
of soils along the perimeter of the structure was redistributed into the lanes
using a blade attachment on the tractor.
8610U/1-FINAL REPORT £-11
-------
TABLE E-3
Analysis of Samples Collected
From the Bioreactor During Test Operations
Concentrations in ng/g (ppb)
KETONES
ACftOfl*
2-Butinone
2-HiMncne
SHORT-CHAIN CHLORINATED
HYDROCARBONS
CMoroforn
tothyltnt Chloride
1,2-Oichloroethint
1.1.2-lrichloro«hant
1,4.2,2.-T«tracMoroftn«nt
1.1-D1cMoPotthent
friehlorotthtnt
TttracMorotthent
Bif(2-CMorotthyl)Eth«r
Htxachlerobutad'tiftt
1 . 1-0ieh1opo*th*nt
1.2-OichlorotthtM
CHLORINATED AJtOKATIC
HYDROCARBONS
CMorobmztnt
1.2-DicMorob«nzcne
1,3-Diehlorooenzwe
1,4-Oichlorobtnzcni
1.2,4-Trichlorobtnztnt
Itexachlocobcnztnt
2-Chloroaaohthaltftt
2-CMoroohmol
ARONATICS .
Total XyltMS
EthylbanxtM
Styrone
2-Atthyliuotthilant
Wnnanthrtnt
Anthracm
Bwuro () anthractnt
Oibouo («,h) tnthractnt
Fluortnt
Fluorowthtflt
IHbwuofuran
8«zo (b) fluoranthana
1st Run
Initial F1n«l
BOL(IO)
BOLdO)
BOLCO)
BOL(5)
1
BOL(S)
It
BOL(S)
BOL(S)
SOUS)
TOL(S)
BOL(12)
801(12)
80L(5)
BOL(S)
BOL(S)
801(12)
«(1)
8DL(12)
BOL(12)
(2.«)
801(12)
801(12)
8DL(S)
11
80L(S)
(M)
U
(fl.»)
BOL(12)
8DL(12)
801(12)
(fl.T)
80L(12)
801(10)
BOLdO)
BOL(lfl)
80L(S)
S
BOL(S)
80t(S)
80L(5)
BtH(S)
BOL(S)
BOL(S)
80L(23)
80L(23)
BOL(S)
80L(S)
BOL(5)
80L(23)
BOU(23)
BOL(23)
BOL(23)
801(23)
BOU(23)
BOL(23)
801(5)
BOC(5)
BOL(S)
80L(23)
(>.s)
80L(23)
80L(23)
801(23)
80L(23)
80L(23)
80L(23)
801(12) BOL(23)
2nd tun
InU1*l Fliul
21
BOL(IO)
BOL(tO)
801(5)
11
801(5)
1
WL(S)
80L(5)
80L(S)
BOL(5)
«(«)
moo
BOt(S)
801(5)
BDL(S)
80L(U)
d.5)
BOL(U)
80L(U)
«(U)
BOL(U)
80L(14)
80L(S)
40
80L(S)
(12)
117
(B.3)
BOL(14)
801(14)
'(»)
12.1]
8M.(14)
BOL(IO)
BDL(IO)
BOL(IO)
80L(S)
B
801(5)
80L(5)
801(5)
BOL(S)
80L(S)
BOL(S)
801(11)
801(1!)
80L(S)
801(5)
BOL(S)
801(11)
80L(11)
80L(11)
80L(11)
IS
BOL(II)
80C(11)
BOL(S)
80L(S)
BOKS)
d>
1C
80L(11)
BOL(II)
801(11)
801(11)
(5)
801(11)
80L(14) ! 801(11)
3rd Bun
Initial
1
1
801(50) 1
BDL(50) 1
BOL(SO) !
i
1
1
!
801(25) 1
53 1
7JO 1
440 |
801(25) 1
80L(25) i
801(25) 1
BOL(2S) |
45 1
801(11) |
BX(2S) I
BOL(25) 1
1
1
1
801(25) |
801(11) 1
BOL(II) 1
BOL(ll) !
80L(11) |
d) 1
801(11) 1
801(11) 1
I
I
1
801(25) i
801(25) i
BOL(») !
(4) I
1JO i
«(D 1
801(11) t
801(11) !
(») 1
(«> i
801(11) 1
801(11} 1
Final
BOLdO)
BOL(IO)
801(10)
BDL(S)
15(6)
80L(5)
BOL(S)
801(5)
80L(S)
80L(5)
BOL(5)
«(2)
B0l(11)
80L(5)
80L(S)
BOL(5)
BOL(lt)
BOL(II)
BOL(H)
BOL(H)
«(S)
BOL(tt)
80L(11)
801(5)
BOL(5)
BOL(5)
801(11)
'(«)
80LC.1)
80L(r)
BOLd!)
BOL(1l)
80L(!1)
BOL(ll)
BOC(tl)
861014/1-FINAL REPORT
E-12
-------
TABLE E-3
Analysis of Samples Collected
From the Bioreactor During Test Operations
Concentrations in ng/g (ppb)
(continued)
3e*zo (k) *':uoraitr>ene
Benzene
"o'ue^e
Naonfttlene
Chrysene
Pyrene
Hn'it'cscaionenylaeine
Benzoic Acis
Acenaonthvlene
tSTW
Viryl Acecatt
Carbon Disul'idt
1st
Initial
.1 i
i
1
BOU12) |
80L(5) !
831(5) 1
(3.5) 1
BDL(12) i
P. 3) i
801(12) 1
(5) i
301(12) !
i
!
BOL(IO) !
!
BOL(S) 1
tun
Final
80L(23) |
801(5) i
801(5) 1
801(23) !
BDL(23) |
801(23) !
801(23) |
801(115) 1
301(23) I
1
1
801(10) I
I
801(5) i
2n4
Initial
1
BOL(U) |
80L(5) 1
«(«) 1
«(10) 1
801(14) |
'(5.7) 1
«00(a) |
801(70) 1
'(1.4) 1
i
1
801(10) I
I
80L(5) I
tun
Final
8CL(11)
801(5)
801(5)
*(*)
801(11)
14
(10)
BOL(SS)
801(56)
801(10)
BOL(S)
Jrd
Initial
BOL(II)
801(25)
BOL(25)
'(1)
*(?)
'(«)
aoL(ii)
(S)
*(2)
BOL(SO)
80L(25)
Kun
Final
' BOL(II)
1 BOL(S)
1 »(1)
' 80L( : )
: BOL(II)
»(3)
i 8DL(11)
: *{4)
' 801(11)
1
1 BOL(IO)
!
! 80L(5)
(i) » This nucber taken froa dilution
(b) - Also found in blank. Possible/orobable contaaination of smole
(c) » Above ouantitation rtnqe
(e) * Coelution of coaponents. total detectable acaunt reported
* > Detected but below the Mthod Quantification liiit (estiaated concentration)
OL(>) > Below Detection Li«it (Estlaated Detection Liait)
861014/1-FINAL REPORT
E-13
-------
b. $oil Moisture Content. The soil moisture content in each quadrant was
monitored twice weekly. When required, moisture was applied to the pilot test
area with the overhead distribution system. Moisture content was measured
with a "Speedy Moisture Tester" or with an oven. During the later stages or
operations, as temperatures inside the enclosure increased, up to 400 gallons of
water per day were added to maintain soil moisture. Soil moisture content was
maintained between 10 and 15%.
c. Soil oH. Soil pH was recorded in each test quadrant twice weekly. Three
grams of soil were mixed with 3 grams (3 ml) of deionized water and the pH
measured with a portable Solimat pH meter. The pH meter was standardized
with pH 4 and pH 7 buffers between readings. Soil pH ranged from 7.0 to 8.9.
d. Air Temperature. Twenty-four hour maximum and minimum temperatures were
recorded daily at 5 separate representative locations in the process area: 1)
inside - south end of treatment structure, 2) inside - north end of treatment
structure, 3) inside - air management system, 4) at the inlet to the air
management system, 5) ambient air temperature taken at the portable electric
box located at the front of the structure. These readings were recorded with
a Taylor Self-Registering Thermometer. Air temperature inside the treatment
enclosure ranged from 33° to 130°F.
e. Soil Temperature. Since very high soil temperature (>100°F) could inhibit
microbial activity the soil temperature was recorded daily in each test quadrant
with a Solimat Portable temperature probe. The probe was inserted directly
into the soil. Soil temperature ranged from 53° to 87°F.
f. Test Area Humidity. The humidity, as measured by the wet bulb-dry bulb
method, was taken at the entrance to the duct leading to the air management
system. These measurements were recorded three times daily as temperature
readings and converted to humidity to provide an indication of moisture
loading rates for the carbon units.
g. Air Quality Monitoring. During the soil treatment study, it was anticipated
that volatilization of some fraction of the organic contaminants would occur.
In order to better characterize the emissions inside and outside the treatment
structure, and to demonstrate the effectiveness of the air management system,
a systematic series of air quality measurements was taken. The readings were
averaged daily for all locations, background concentrations, and at treatment
facility locations.
Air quality measurements for total organic vapor were taken using a Foxboro
Century OVA 128 CC. These measurements were taken at various locations
inside and outside the greenhouse structure as follows (Figure E-S):
o 4 sample locations - each corner inside greenhouse
o 3 sample locations - midpoint of each row of arch support pipes
o 13 sample locations - outside greenhouse
861014/l-FINAL REPORT E-14
-------
FIGURE E-5
Air Monitoring Locations N
EXPLANATION
ANCH NUMBER
L - LEFT
M MIDDLE
R - RIGHT .
A - 8'
8. 4'
~
OF BLDG. C_
ABOVE SOIL
SURFACE
c - r J
.
w
CARBON
K
2
O
£
z
O
o
c
o
X
Ul
EMISSION
CONTROL
SYSTEM
^29 LA
29 LB
29 LC
17 LA
17 LB
»~17 LC
1 LA
1 LB
^ILC
17 MA
17 MB
,.17 MC
s
o
LEACHATE SUMP
MICROBIOLOQICAL
SUPPORT SYSTEM
.BACKGROUND
29 RA
29 RB
29 RC
15 RA
15 RB
15 RC
1 RA
1 RB
1 RC.
1
/^7\
\C\s
fi
'
PIT o
E
::: ::~«. !
F r n v A
8 ETJ T"- Kflj
BRIO SITE TASK FORCE
BRIO PROCESS AREA
AIR MONITORING LOCATIC
161014/l-FINAL REPORT
E-15
-------
The air quality on the exterior of the treatment facility did not exceed
background concentrations except for a breakthrough on February 9. The
breakthrough was 4 ppm above background conditions (Figures E-6 and E-7).
Air quality measurements taken inside the treatment structure were, in all
cases, below the applicable ACGIH TLV (Table E-ll). The two methods for
sampling, i.e., organic vapor monitors and charcoal tubes, were compared for
selected contaminants (Figure E-8 and Table E-4). The differences between
the measurements were not significant based on a paired T-test (p < .001).
The measurements taken with the OVA show that the concentration of
contaminants was reduced over time.
h. Chemical Analyses. The analytical chemistry data collected during the
operation of the pilot test is discussed in Section 5 and presented in detail in
Appendix F.
861014/1-FINAL REPORT E-16
-------
O
>
CONCENTRATION (PPM)
o
u
a
*
e>
oo
A
I >
<
? =
fl
o
>
O
n
o
n
03 _»' _
00
x>
m
P
s:
a
to
CD
v|
n
z
o
o
««
50
£'
f j
-------
81-3
1VNIJ-1/H0198
CONCENTRATION (PPM)
m
X
O
B)
n
«r
o
3O
70
O
fil
<»
o
3
rr *
:
O U>
3 »
"I
u
3
U
oo
o
3
n
a
to
x:
w
«
2,
o"
o
i!
32
e <
a
-------
61-3
1MOJ3H 1VNIJ-1/K0198
CONCENTRATION OF TOLUENE (PPM)
g P P g o P P P P
_QOPP»^^-*^
PK»4bOlGl-*rO-|t0iCB
I I i I I
8
s
01
CD
m
o
s
m
Ol
Ol
s
///////////////,
97
O
OB
Ty
o
e
o
5 *>
HS n
2.a e
5 »
3 -PJ
S?«
3 «
e^
a*
10
A
5
o
&
w
-------
TABLE E-4
Comparison of Air Sampling Methods
Pilot Test Treatment Facility
TINE WEIGHTED AVERAGE (Bg/«3)
DATE
2/19
2/26
3/5
3/10
3/19
3/26
4/2
SAMPLE
NUMBER
0354
0391
0415
0306
0336
0436
0307
LOCATION
EXHAUST
EXHAUST
EXHAUST
EXHAUST
EXHAUST
EXHAUST
EXHAUST
DUCT
DUCT
DUCT
DUCT
DUCT
DUCT
DUCT
TEMP
(F)
51
57
61
64
95
80
100
TIME IISOPROPYL
(in) (BENZENE
' OVM
90 i 0.9
90
90
90
90
90
90
2.6
1 .7
0.4
0. 1
0. 1
0. 7
CT
0.5
1 .3
0.2
0.2
0.2
0.1
ETHYL
BENZENE
OVM
2 .3
5.8
2.7
0.4
0.4
0.2
0.61 1.2
CT
2.0
3.6
0.2
0.2
0.3
0. 1
1 .4
TOLUENE
OVM
0.3
3 .0
0.3
0.3
0. 1
0.2
0.5
0
0
0
0
0
0.
0
OVM - Organic Vapor Monitor (3M 3500)
CT - Charcoal Tubes
861014/1-FINAL REPORT
E-20
-------
TABLE E-5
Soil Moisture - Ecova Brio Process Area
(Moisturts in perctnt by wight)
C1 C2 C3 C4 N1 N2 N3 Nl II 12 13 14 HI N2 M3 N4
FEBtl S.I 10.1 10.4 9.( 10.0 10.0 11.2 10.1
FEB12 4.1 11.2 1.0 11.2 11.1 12.2 14.C I.I
FEB14 9.2 10.4 10.2 10.1 S.i 11.2 10.4 11.1 11.4 11.0 11.1 12.1 15.5 14.1 17.4 IS.4
FEB17 S.S 4.1 S.5 10.5 4.1 14.0 VI 10.1 7.1 1.2 I.S 11.1 7.5 1.4 I.I 10.4
FE820 f.l 11.4 10.5 13.7
FEB22 1.4 I.I 10.0 1.4 1.4 11.2 10.( 1.2 11.2 13.2 1.0 10.3 10.4 1.4 1.2 11.7
FEB23 9.1 I.I 1.3 1.1 I.I 10.3 11.4 11.1 11.0
FEB24 7.1 1.2 10.0 11.4 11.5 10.1 1.2 I.I
FEB2S 9.1 10.3 11.5 11.0 10.( 11.1 11.1 12.9 11.1 13.4 12.1 11.3 13.2 13.0 13.4 12.1
FEB27 10.4 10.5 11.3 10.1
FEB2I 10.1 10.3 11.4 11.5
MM2 9.0 36 9.5 9.( 10.0 11.7 11.3 12.2 11.1 12.0 12.0 11.1 10.S 10.1 I.I 11.0
NAR04 10.1 10.2 11.5 10.S 11.1 12.1 11.5
UR05 12.2 10.5 12.4 12.1 12.7 12.1 12.4 13.4 17.1 11.7 14.1 13.0 12.1 13.3 12.2 11.2
NAMf 12.1 14.4 13.4 13.1
MAROI 12.3 10.3 11.7 12.5 11.1 10.1 10.1 10.5 10.2 11.2 11.1 13.1 10.7 12.4 12.1 13.1
MARIO 13.( 12.2 10.1 11.4
HAR11 11.S 11.1 12.0 12.1
XAR12 10.7 9.3 9.5 9.110.210.210.0 9.111.311.1 1.411.013.712.213.111.1
HAR15 13.4 11.0 10.1 10.1
NAR16 10.2 9.4 10.4 10.5 11.5 11.2 13.1 12.1 11.2 12.4 11.1 10.0 10.4 12.0 10.2 11.1
MR1I 10.1 10.4 12.1 11.4
NAR20 10.i 12.0 11.5 11.3 12.1 10.9 10.1 10.1 12.4 13.4 13.1 13.2 13.2 11.2 11.1 13.1
MR24 10.5 10.4 13.1 10.1 11.4 12.4 10.1 10.1 12.1 11.0 11.1 11.1 10.0 l.t I.I
HAR2S
MR2S 14.2 -.2 11.1 10.2
NAR27 1).$ 12.4 10.4 10.9 14.1 15.2 13.4 12.3 14.5 12.4 13.2 1S.O 12.7 11.2 12.1 11.4
Mft31 1.0 It.S 10.5 11.4 12.3 10.4 10.0 13.0 11.2 10.2 12.1 10.1 10.1 12.2 11.1 13.1
AM02 10.1 10.2 10.1 14.2
AMOS 12.0 12.4 12.0 12.0 9.5 10.0 11.4 12.1 1.4 10.1 12.1 12.0 11.1 11.1 11.1
APM4
AFROS
APMI
APR07 I.I 11.1 10.1 11.f 12.2 10.1 11.2 10.4 10.4 10.1 13.1 10.5 10.2 13.1 11.1 12.4
APMI
AFWI
AFH10 11.1 10.1 11.1 11.2 11.1 12.2 12.1 11.1 12.1 15.2 12.1 11.5 1.4 11.2 12.2 12.2
Attll
APR12
APR13 12.4 10.S 11.1 11.1
S61014/1-FINAL REPORT E-21
-------
TABLE E-5
Soil Moisture Ecova Brio Process Area
(continued)
C1 C2 C3 C4 N1 N2 N3 N4 II 12 13 U Ml 1C tt M
AM14 11.1 I.I 10.1 11.S K.I IS.4 11.0 1.4 11.2 10.1 11.1 11.1 11.1 12.0 11.1 11.1
AMIS
AMU 11.1 11.3 12.2 12.0 11.1 12.4 13.2 12.1 12.1 12.4 12.3 10.S 11.1 11.2 11.2
AW 17
AMI*
APHIS
AM20
APR21 11.1 14.2 14.7 14.1 19.t 12.7 14.2 12.1 13.0 11.1 21.S 12.1 13.S 1S.O 11.3 11.7
AM22
APR23 11.7 12.0 12.1 12.1 11.1 1S.O 13.7 U.I 13.S 14.S 14.1 13.4 14.1 13.i 13.7 14.S
AM24
APR2S
APR26
APR27 21.S 12.1 It.6 12.0 12.1 11.1 14.4 13.7 10.1 14.4 14.2 12.1 13.S 14.0 11.3 IS.I
APft28
APR29
AM30
861014/1-FINAL REPORT E-22
-------
TABLE E-6
Soil Temperature - Ecova Brio Process Area
(7Mptritures 1n Dt^r*** F)
DATE
Ct
C2
C3 C4
N3
11
13
II
1
N2
n M
FE8D1
FEM2
FEB03
FEB04
FEBOS
FEB06
FE807
FE808
FEB01
FEB1I
FE812
FEB13
FE814
FEB15
FEB16
FEB17
FEB18
FEB21
FEJ24
FEB27
HAR02
NAR06
NAR09
MD11
NAR12
NAR20
NAR24
MAR2&
MAR26
MAR27
KAR28
MR29
NAR30
MR31
APWI
APW2
AW03
AM04
APROS
AM06
APR07
AM09
APR10
APR12
63.5 63.9
60.1 S9.5
S9.7 60.1
59.4 61.0 59.7
61.0 61.2 63.5 64.6 61.3 63.0
62.2 62.2 62.4 62.6 62.2 62.2
73.4 75.2 77.0 77.0 73.4 75.2
62.6 62.2 62.6 63.3 64.6 63.5
56.8 56.1 57.2 57.2 56.5 58. 5
70.3 67.8 67.6 71.4 76.3 67.6
75.6 76.3 77.2 79.5 74.3 76.3
73.9 75.6 75.6 79.2 72.7 74.3
75.6 7S.2 77.2 78.1 74.3 74.3
64.0 64.6 66.0 66.6 63.0 64.4
72.0 72.3 73.0 74.3 72.1 73.6
64.6 64.4 64.0 66.4 64.0 64.6
65.1 65.7 68.2 70.5 65. 3 66.0
54.0 54.0 54.9 54.5 54.5 53.6
60.1 59.7 61.7 63.5 60.6 59.7
72.9 73.4 73.4 77.0 75.2 75.2
64.6 66.6 69.1 74.7 69.8 75.2
78.0 72.0 75.0 79.0 78.0 76.0
77.0 80.0 78.0 12.0 78.0 76.0
56.0 56.0
57.0 58.0
66.2 67.8 70.7 72.3 69.4 69.0
71.5 73.5 73.5 80.1 76.0 70.7
71.5 61.1 71.5 72.6 71.5 69.1
61.0 66.2 TO. 7 73.9 69.4 66.2
73.0 79.0 82.0 81.0 76.0 73.0
61.2 66.3 69.9 73.1 69.6 68.2
72.3 73.6 77.2 77.2 76.6 74.3
62.8
62.4
75.2
65.1
58.S
68.5
76.3
75.9
74.8
64.4
74.3
64.6
69.1
54.5
61.3
75.2
75.2
78.0
77.0
67.8
75.6
72.7
71.5
77.0
70.3
77.2
61.0
63.0
62.6
77.0
65.1
56.1
72.0
79.2
80.1
74.5
64.4
76.6
66.9
70.5
56.1
63.0
77.0
75.2
78.0
81.0
71.9
80.1
76.4
74.3
17.0
73.1
71.4
64.2
51.4
61.2
59.7
62.2
62.4
73.4
65.3
S8.5
66.2
73.9
75.4
74.3
63.3
71.4
64.2
64.2
54.9
60.1
75.2
66.6
76.0
66.2
74.7
73.1
69.0
76.0
61.1
73.1
63.0
62.6
77.0
85.5
57.7
65.5
76.3
75.4
74.3
64.1
75.6
64.4
6S.8
54.T
51.4
T5.2
70.7
71.0
72.0
62.0
67.4
73. S
73.9
66.1
7T.I
61.4
T3.1
mum
63.
62.
T8.
65.
SI.
66.
76.
77.
74.
64.
75.
64.
61.
54.0
51.1
TS.2
74.3
71.0
70.3
71.4
73.1
71.S
7T.1
T1.S
TS.I
nan
61.
63.
62.
80.
65.
59.
69.
79.
78.
74.
65.
77.
67.
71.
55.
62.;
TT.I
T0.5
Tl.l
71.1
11.9
6l.i
T).!
O.I
T4.1
TS.I
isRrai
64.
60.
61.
56.
62.
62.
77.
64.
59.
66.
75.
T3.
T3.
64.
T1.
64.
64.
1 54.
t 61.1
) TS.;
i is.:
I T8.I
1 61.1
» TT.I
\ 78.;
i 76.!
1 TT.I
T0.1
r TI.I
mnRiw
63.
62.
77.
64.
SI.
17.
75.
TS.
TS.
14.
TS.
(4.
66.
1 55.2
) 59.T
! TS.2
1 66.9
) 78.0
) 71.3
1 7S.2
t 78.8
1 T1.S
1 M.O
1 72. T
t 75.6
1SXXXXXJS
63.
62.
77.
65.
59.
68.
77.
78.
76.
64.
76.
65.
66.
SS.2
60.8
75.2
68.5
78.0
62.0
69.4
10.1
71.0
73.1
12.0
71.1
tt.T
xszsxx
61.0
63.0
62.6
80.6
65.7
59.0
70.2
79.0
71.1
TS.6
16.4
75.4
16.7
68.0
55.8
61.T
77.0
72.3
78.0
71.1
80.5
71.8
T3.S
12.1
T3.I
73.8
161014/1-FINAL REPORT
E-23
-------
TABLE E-6
Soil Temperature - Ecova Brio Process Area
(continued)
DATE C1 C2 C3 C4 N1 N2 N3 N4 11 12 13 (4 Ml N2 M3 N4
^
APR! 7 77.0 77.0 77.0 71.0 74.0 73.0 76.0 76.5 74.0 74.0 7S.O 77.0 7f.O 76. C 76.0 77.0
AM20
*HB1 75.0 76.0 71.0 71.0 77.0 76.0 7S.O 10.0 71.0 77.0 76.0 71.0 77.0 76.0 76.0 79.0
AP822
AM23 76.1 76.4 72.3 7S.6 76.0 76.4 76.0 60.1 79.6 76.4 76.6 60.9 76.4 79.2 77.6 79.2
AM27
AW»8 76.0 75.2 77.6 76.4 74.7 77.6 76.4 77.6 61.3 60. 5 62.S 60. S tl.S 61.3 79.2 79.2
APR29
AM30
861014/1-FINAL REPORT £-24
-------
TABLE E-7
Soil pH - Ecova Brio Process Area
C1 C2 C3 C4 N1 N2 N3 Nl 11 12 13 14 HI « M M
ssrss*rsrsrr«zs«=s2Ss=ssr=s=s=r=rrsss=issss=Bssssissssss»sss:s=sssi=sssrrsssxas»
JAN31 7.5 7.2 7.3 7.4
FE8Q4 7.J 7.2 7.0 7.0
FEB05 8.6 8.5 8.4 8.8 8.S 8.5 8.4 8.7 8.4 8.4 8.4 8.8 8.8 8.5 8.4 8.7
FE806 7.2 7.1 7.2 7.2 7.3 7.1 7.4 7.4 7.3 7.3 7.4 7.4 7.1 7.1 7.5 7.1
FEB09 8.5 8.5 8.6 8.7 8.8 8.9 8.9 8.6 8.6
FEB10 8.5 8.7 8.1 7.8 8.2 7.9 8.0 7.0
FEB12 8.6 8.7 8.7 8.2 8.7 7.6 8.9 7.7
FE814 8.7 8.9 8.5 8.2 8.6 8.5 8.8 8.5 8.4 8.9 8.6 8.7 8.8 8.2 8.5 8.9
FEB17 8.7 1.6 8.6 8.6 8.3 8.4 8.7 8.2 8.5 8.7 8.7 8.4 8.6 8.8 8.8 8.3
FE822 8.3 7.9 8.5 8.0
FEB23 8.3 8.5 8.2 8.2 8.3 8.7 7.8 8.3 8.6 8.0 8.3 6.6 8.6 8.2 7.9 8.4
FE825 8.2 8.2 8.5 7.5 8.3 8.3 7.7 7.9 8.1 8.4 8.0 8.6 8.1 8.2 8.0 9.0
FEB2T 8.3 8.4 8.0 T.9 8.2 7.9 7.7 8.0 6.3 1.0 7.9 8.1 7.9 8.2 8.0 7.9
NAR03 8.4 8.2 8.0 8.1 8.1 8.1 8.0 8.3 8.2 8.3 8.1 8.2 8.3 8.2 8.1 8.2
NAX06 8.0 8.3 6.4 1.4 8.8 8.5 8.4 8.4 8.5 8.5 8.2 8.5 8.3 8.S 8.2 8.5
NAR09 8.5 7.9 6.4 8.0
MARIO 8.1 8.2 8.0 8.1 8.0 8.3 8.0 6.3 8.2 8.0 8.1 8.0 8.1 8.2 8.0 8.0
NAR13 8.5 8.5 8.3 8.2 8.3 8.3 8.1 8.3 8.4 8.5 8.2 8.4 8.3 6.3 6.1 8.4
NAR16 7.9 1.1 8.1 8.1
MR18 6.4 1.4 6.2 8.2 6.3 8.2 8.2 9.2 1.3 6.2 8.1 8.3 8.3 8.3 8.1 8.2
NAttO 1.1 8.2 7.6 7.9 7.9 8.0 7.7 7.7 7.9 7.8 7.6 8.3 7.7 7.1 7.8 7.6
NAR24 8.0 T.9 7.9 7.9 8.0 8.1 7.9 8.0 8.0 8.0 8.1 8.1 8.0 8.2 6.1 8.0
NM25
Nfttti 1.0 8.2 8.0 8.0
MAJH7 8.1 8.0 8.0 8.1 7.9 8.1 7.9 7.9 8.0 8.1 7.8 6.1 8.0 8.1 8.0 8.0
NAR31 7.1 1.0 8.0 8.1 8.0 6.2 6.0 1.0 6.0 6.0 7.6 7.9 7.6 8.0 7.9 7.8
APR02 7.8 7.9 7.8
APR03 8.1 0.1 8.1 8.0 8.1 8.2 8.2 8.1 6.0 8.0 7.9 T.8 7.9 0.0 7.9 8.0
APM4
861014/1-FINAL REPORT E-25
-------
TABLE E-7
Soil pH - Ecova Brio Process Area
(continued)
C1 C2 03 C4 N1 N2 N3 N4 II 12 13 14 N1 K N3 M
APR06
APR07 6.0 8.0 8.0 8.1 8.0 8.0 7.8 8.0 8.1 t.1 8.0 8.1 8.1 8.2 8.1 8.1
APR08
APR1C 8.C 6.0 7.9 7.8 7.9 8.0 7.9 7.9 7.8 7.8 7.7 7.8 7.8 7.8 7.8 7.8
APR12
APR13 7.5 7.7 7.7 7.8
APR14 8.3 8.2 8.2 8.2 8.2 8.3 8.2 8.3 8.1 1.1 8.0 8.1 7.6 1.1 8.1 8.0
APR15
APR16 8.1 7.9 7.9 7.9 8.0 7.9 8.0 7.9 7.9 7.9 7.8 7.9 7.8 7.9 7.9 7.9
APR17
APR21 7.7 7.8 7.6 7.7 7.8 7.8 7.7 7.8 7.8 7.8 7.8 7.8 7.9 7.8 7.9 7.8
APR22
APR23 7.9 8.0 8.1 8.1 8.2 8.2 8.2 8.1 8.2 1.2 8.1 8.2 8.2 8.2 8.2 8.2
APR24
APR28 7.7 7.5 7.6 7.5 7.8 7.7 7.7 7.9 7.9 7.8 7.9 7.8 8.0 7.9 7.9 7.9
AW29
APR30
861014/1-FINAL REPORT E-26
-------
TABLE E-8
Air Temperature - Ecova Brio Process Area
(Tnpcnturis 1n Dtgrm F)
DATE
AMBIENT
MX
JAN31
FEB01
FE802
FEB03
FEBC4
FEBOS
FEB36 60
FEB07 65
FEBOS 75
FEB09 70
FEB10
FEB11
FE812
FE813
FEB14
FE81S
FEB16
FE617
FEB1I
FEI19
FEB20
FEB21
FE822
FEB23
FEB24
FEB25
FE826
FEB27
FEB2I
MR01
MM2
MR03
MR04
MROS
MROi
MR07
MROI
MR09
MR10
MR11
MR12
MR13
MAR14
MR15
MR16
MR17
NAR1I
MR19
MR70
MR21
HAX22
MR23
65
12
II
14
10
12
71
II
70
41
SO
SI
17
II
56
59
64
10
79
71
12
76
10
10
13
12
14
90
51
73
74
II
13
13
IS
92
II
90
11
11
NIN
SO
50
SO
55
47
45
72
SO
49
49
41
41
33
42
41
54
46
SO
SO
S3
SI
60
52
44
46
41
43
44
41
SO
49
SO
41
39
39
40
64
62
SI
52
51
55
54
63
66
STRUCTURE
NORTH
MAX
IS
7S
85
76
75
105
17
10S
120
93
19
96
104
102
94
91
94
IS
99
57
56
74
96
10
64
60
73
87
106
110
107
101
110
114 '
101
99
101
112
91
71
110
111-
104
104
94
117
117
119
125
130
104
101
HIN
II
55
55
SI
62
60
SS
SS
60
SO
SI
56
54
10
II
49
41
34
43
52
46
46
SO
52
59
56
SI
52
44
4S
47
II
43
44
47
SO
40
50
51
31
40
40
14
11
60
52
51
54
54
14
66
STRUCTURE
SOUTH
MX
91
100
91
90
100
16
14
94
53
SI
74
99
72
SI
60
69
II
103
109
101
101
110
114
100
91
110
101
91
75
107
100
102
97
94
111
111
120
122
130
104
102
HIN
54
52
54
SI
SI
47
44
34
43
43
41
41
50
52
10
56
51
51
43
12
41
14
43
44
41
SO
49
SO
51
31
39
41
13
11
11
SO
51
54
54
13
65
AIR
MX
96
94
74
II
94
12
10
90
SI
54
72
91
SI
60
62
69
14
91
111
100
104
105
110
92
S3
103
100
94
75
102
91
100
99
91
109
111
113
111
121
100
AIRMNAGEMENT
IN SYSTEM
an »***»»** i -
MET
BULB
B**4W:
U 1
DRY
BULB
tmx**:
RELATIVE
HUMIDITY DATE
HIN MX HIN AM NOON M AM NOON W
51
SI
74
56
60
41
41
33
49
SO
SI
46
51
52
54
SI
51
52
43
14
41
47
42
44
41
SI
41
SO
51
31
31
40
13
11
61
51
51
54
SI
13
67
71
71
92
10
14
72
70
IS
SI
52
II
19
59
14
75
II
10
17
14
IS
II
10
70
79
14
77
56
76
74
10
13
90
17
94
17
14
10
71
71
SI
70
02
12
43
41
53
41
12
51
57
64
65
II
70
71
12
N
14
44
II
11
41
10
41
SI
43
44
S3
12
SS
60
11
54
74
71
62
12
II
12
10
SO
44
41
45
41
74
41
SI
S3
SI
11
72
71
12
52
S3
SS
52
IT
IT
14
10
41
S2
54
T2
T2
11
57
55
10
70
79
79
12
79
II
71
11
12
60
10
41
41
11
10
SI
SI
62
12
12
12
11
It
14
14
12
11
13
13
12
71
N
71
94
101
12
7S
75
00
70
11
12
II
II
M
41
52
52
S4
51
12
19
11
11
11
14
II
14
12
11
11
IS
54
12
II
M
12
M
II
12
11
M
112
10
14
14
II
14
12
54
41
41
41
SI
11
S3
52
54
57
12
IS
14
IS
SS
10
11
SI
14
12
II
14
51
SI
SI
11
n
M
13
SI
14
14
11
13
II
12
II
14
II
10
M
14
SI
50
13
14
SI
10
14
14
12
M
12
92
112
II
12
N
11
11
II
14
13
11
IN
111
II
II
71
IS
74
10
II
74
72
11
SI
53
51
Si
SI
14
19
12
II
II
91
101
N
10
II
M
10
SI
10
11
11
13
12
112
IS
14
112
115
12
AH NOON
90
91
IB
90
II
71
71
It
14
11
13
11
17
94
94
IS
17
12
IS
12
13
II
12
10
111
11
10
11
11
11
3
11
IS
11
II
12
10
14
14
II
12
77
70
II
10
SO
IT
13
71
10
19
II
90
91
II
10
14
IS
74
57
12
70
IS
19
11
11
12
11
59
71
55
19
PM
JAN31
FEB01
FEB02
FEB03
FE3C4
FEBOS
FEB06
FEB07
FEBOI
FEB09
FEB10
11 FEB11
11 FEI12
12 FEB13
13 FEB14
72 FEB1S
74 FEB16
12 FE817
IT FE81I
IT FEB19
94 FEB20
FEB21
FEB22
IT FEB23
IIFEB24
94 FEB25
91 FEB26
IS FEB27
12 FEB2I
MR01
11 MR02
14 MR03
SI MR04
57 MROS
41 MR06
14 MR07
10 MROI
92 MR09
19MR10
91 MR11
T1 MR12
16 MR13
TSMR14
S9MR1S
I2MR16
52 MR17
54 MR1I
73MR1J
SI MR20
14 MR21
MR22
II MR23
8610H/1-FINAL REPORT
E-27
-------
TABLE E-8
Air Temperature - Ecova Brio Process Area
(continued)
DATE
xxxx:
MR24
NAR25
MR26
NAH27
AMI
NAR29
NAR30
MR31
APR01
APR02
APR03
AM04
VMS
AMOf
AW07
APWI
APR09
AM10
APR11
APH12
APR13
AMU
AMIS
AMIS
AM17
AMU
APR; 3
APR20
AM21
AM22
APR23
AM24
APR25
APR26
APR27
AM21
AHttI
APR30
AMIENT
Bszsnsx*
MX
II
II
If
10
II
(2
II
It
It
71
73
IT
17
10
II
II
12
10
17
104
100
IS
S3
II
95
If
15
13
1M
N
IN
4$
SI
Sf
S3
SI
51
31
32
4f
41
34
37
41
II
45
41
57
14
71
51
41
55
SI
51
to
57
10
51
10
10
57
11
11
STRUCTURE
NORTH
UXUMSS
MX
111
111
114
117
130
10
10
112
120
111
lit
104
117
117
111
111
121
1N
110
124
124
131
12S
12$
130
130
121
130
127
1M
W
NIN
45
SO
51
54
54
53
41
34
47
50
31
31
51
47
45
41
Sf
IS
II
SO
4}
54
SI
10
to
57
12
SI
10
10
SI
10
14
STRUCTURE
SOUTH
*********
MX
til
113
114
114
123
71
IS
112
122
114
111
107
114
121
HI
111
111
101
112
127
127
130
120
120
133
121
130
130
121
130
111
NIN
45
SI
55
S3
S4
S3
31
33
47
SO
35
40
SO
41
4S
41
Sf
14
II
41
41
S3
SI
to
to
57
12
SI
SI
SI
SI
N
13
AIR
xxm
MX
112
112
111
10*
111
10
10
106
116
110
101
102
101
112
112
11$
111
IK
101
122
121
130
124
112
130
123
121
130
123
123
114
AIR
IN
ten i
MNA6EMENT
SYSTEM MET BULB
its****** ************
NIN MX NIN
44
SO
ss
S3
54
S2
31
33
47
SO
35
31
51
41
45
45
Sf
(4
54
51
41
S3
Sf
fO
11
57
12
SI
SI
to
fl
10
13
71
II
12
It
71
14
13
17
12
13
15
11
13
101
13
122
101
114
104
II
110
14
110
M
1DS
(Of
102
M
57
ts
S6
S3
51
41
34
52
16
57
52
Sf
SI
41
70
72
72
12
11
10
12
57
13
14
13
71
71
70
72
AM NOON
10
to
Sf
SI
71
Si
39
S4
If
63
SI
51
52
55
SI
ff
fl
73
52
SI
10
73
70
71
SI
10
51
to
74
10
14
65
77
13
13
100
74
74
II
16
76
74
12
17
10
12
12
71
II
II
II
100
114
II
101
10
Sf
12
II
m
70
it
12
14
10
f6
16
100
72
12
77
16
12
II
12
10
12
101
100
II
II
II
14
14
101
114
11
IS
DRY BULB
************
All NOON
63
II
14
12
77
IS
45
Sf
72
ff
62
56
56
61
62
70
70
71
57
64
67
17
73
14
10
12
42
63
IS
11
»
73
17
17
13
111
71
If
110
112
If
14
11
10
14
12
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14
II
100
If
110
111
II
110
100
101
102
110
PN
If
M
107
lit
111
71
103
111
77
17
14
101
10S
107
114
IT
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116
120
114
112
100
113
123
116
122
122
115
RELATIVE
HWIOI nr DATE
zsxscssrzsxs x:ix:
AN
14
13
fl
71
75
70
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II
73
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71
71
77
II
71
11
10
17
72
75
67
51
If
77
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1)
14
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66
62
76
fS
NOON
64
Sf
ff
S3
13
57
ts
S6
63
13
61
51
SS
ff
SI
77
SO
IS
71
70
II
61
14
42
13
fl
42
«
17 MR24
47 NAR2S
57 MR2f
SO NAR27
45 MR2I
MAR29
SO MR30
50 KAMI
S3 APR01
79 APR02
53 APR03
AW<
AMOS
73 AMOf
45 APR01
fl APMI
47 APROS
44 AM10
AM11
AMI 2
41 APR13
If APftU
71 APR1S
SO APR1f
31 APR17
APR18
APR1I
39 APR20
IS APR21
4> APR22
34 APR23
SI AM24
APR2S
APR26
7S APR27
25 APR2I
29 APR2I
APR30
861014/1-FINAL REPORT
E-28
-------
TABLE E-9
OVA Monitoring - Ecova Brio Process Area
g
E>
Sm
- s
.
** * *^^*^^**^****^^^^MMM^W^W^^WWWWWvWwWWW^WW-»-»W«.vM>
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S :
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25
s
- : *>-
H 1 1
861014/1-FINAL REPORT
E-29
-------
TABLE E-9
OVA Monitoring - Ecova Brio Process Area
(continued)
i. " :
5 _ !
= U mm
£ ft " ^^
-a : -
M * ** ^
** *
j '*l^"-<""*"p-~'"ww~^«;«J!<;«"i»i~~~w2«;Zi«I;3Ip!«»2S2222«Ii"»'«!»J!
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Sc«*MW«w««M»w«»iAvW<^W*>«W«MMiM««*«»*w»wMWmWW*w^^w^*i*»*kWM*»W^wWw<
I;
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R ^^WmWvWWv«W^^M^^WWW«%*^«^<|*«MmvW«*W«i>'tf|»W«*W^«*iWWWWW***»««v«it«Mtf»W^«wM*«^
= 1
r :
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v B Pt«i«^M**W«»W<««>W^«M«WV*V^Mm^^^W4wW«»W«W*«*tfiWW4^«WW«»«li»«BfM«>P«^r*WMW»A**^««^
i!..-.....-..«^«...~^^, ».-.»...-- «.r..»«.......r«~*-..*>
«*M»«*W**«««w^Wk^^i^i«*«>M««««^M>n»^««>^*«vwmmf>>^rWW^M«»«**»WM^WM*»*|**^*«^M«*«i^**w**
i! I i i ii I i i I I ! i I I 1 I I 1 i I I i i I I I I
I
S6I014/1-FINAL REPORT E-30
-------
TABLE E-9
OVA Monitoring - Ecova Brio Process Area
(continued)
a
1 ;!
s S 8
5'-
,
In!
_* m N
*«vw*w«»«w^*»*ii«^M W»
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UM ^i*» ^*»**t«t^»*P*W»W»w ^^^^«*»
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861014/1-FINAL REPORT
-------
TABLE E-9
OVA Monitoring - Ecova Brio Process Area
(continued)
gg M W""»i*«t« > « *D*
*.
: m.'
.i
?
i i I I i
S61014/1-FINAL REPORT
E-32
-------
TABLE E-10
Brio Watering Schedule
of H20
N6
»7
Ml
M
NIC
Hit
M12
HI 3
Mil
Mti
M16
N1T
Mil
Ml 9
M20
M21
M22
W3
M24
M2S
M2S
«T
N2I
K29
M30
M31
At
A;
A3
A4
AS
Ai
AT
At
A9
AID
A11
AN
All
All
A1S
All
AIT
Ail
Alt
A70
A21
UJ
A23
A24
AJ5
A2I
A27
All
A29
c
ICC
too
too
100
too
0
too
100
0
0
too
100
0
0
0
too
ICO
0
0
0
too
100
0
too
100
c
0
100
too
100
too
too
too
0
0
IOC
too
too
100
too
101
0
110
200
too
too
ISO
3
12$
100
tso
100
tso
0
0
0
w
toe
N
100
too
tc:
toe
too
c
MA
ICC
3
c
too
IOC
0
c
0
too
ICO
0
c
0
too
100
0
too
100
0
0
100
too
IOC
19C
too
too
0
0
100
too
too
too
too
too
c
too
20C
'V
* *
In
i
125
100
ISO
ICC
100
0
0
c
V
IOC
;
A
* *
i W
3
:3
c
too
toe
0
*
W
too
too
A
I*
0
0
100
ICO
0
0
0
100
too
0
100
too
0
0
too
too
toe
100
ICC
too
0
0
130
co
30
oc
;30
:oo
c
S3
230
so
sc
ISO
0
"25
100
ISO
130
too
c
A
0
0
ICC
«
A A
wv
oo
'30
tec
100 M !*OC
V
:C3
13:
3
0
100 N 1MCC
3
0
0
130
too
0
0
0
too
too
0
toe
too
too
0
too
too
too
too
too
0
0
0
13C
'SO
too
too
13C
too
A
V
too
203
too
too
00 M INOC
0
125
110
'SO
100
too
0
0
*
3
too
SJK
130
4::
«co
43C
300
c
400
130
0
0
300
40G
3
0
0
400
430
0
0
0
400
400
0
400
400
100
0
400
400
400
400
40C
330
0
0
400
t:o
4CC
400
<:c
430
o
400
103
4;:
toe
55C
«
It
Sflfl
43C
too
400
4co
0
c
e
o
400
161014/l-FINAL REPORT
E-33
-------
TABLE E-ll
Air Quality Sampling Results:
Pilot Test Treatment Facility
1
^
e u
1:1
< u
*?!
X M
! §
ill
! i
1« in « t-
o r»
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sssi^l
25«S2S«;,
M W W W W W ^
§28
* 0
u « £
41 9 -> O
S U I.
861014/1-FINAL REPORT
£-34
-------
APPENDIX F
Analytical Chemistry Data:
Pilot-Scale Evaluation of Solid-Phase Biodegradation
-------
APPENDIX F
Analytical Chemistry Data:
Pilot-Scale Evaluation of Solid-Phase Biodegradation
861014/1-FINAL REPORT F-l
-------
TABLE F-I
Analysis of Samples Collected
From the Control Lane During Test Operations
Concentrations in ng/g (ppb)
srsc.
: :: = = X =
a * s a - *
8888 8
5 £55 i 5
8*88 2
'888888
555555
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l^iflllflllf5!!
8888 I
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k *» ** *« ** M **
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iiil i
* *«^ » ««»
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ll !
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s
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1
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861014/l-FINAL REPORT
F-2
-------
TABLE F-l
Analysis of Samples Collected
From the Control Lane During Test Operations
Concentrations in ng/g (ppb)
(continued)
IM
)
^ti
feM
OMMI
«'«
hn»
i-ittr
1 I
if I
S61014/1-F1NAL REPORT
F-3
-------
i!
TABLE F-2
Analysis of Samples Collected
Froir the Inorganic Nutrient-Adjusted Lane During Test Operations
Concentrations in ng/g (ppb)
lisa a
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rr s s S is s s
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*w ^ w *v ^ 8 ^* ^ *" v v *"' ^* ^ ^ ^
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§111111
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frilrlllllll
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««(») I!N(») MM(t) MM(k)
IH(IMM) Ml(IMN) IIM(k> IH(}NN)
MIIMN) MM INN) M(IMI) Ml(JMM)
iiiiiiiiiiii
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liPIillllil
i~i i iaiiii
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a* aaaaaaaa
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86IOU/1-FINAL REPORT
F-4
-------
TABLE F-2
Analysis of Samples Collected
From the Inorganic Nutrient-Adjusted Lane During Test Operations
Concentrations in ng/g (ppb)
(continued)
ii
i
I
i
H
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g
1
ifiiiiii
II- II- 1
iiilillli
fifii'gf
niiiifii
I rilil|l
i
I I
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2 S
8 *
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= £
I *
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s £
i i
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S61014/1-FINAL REPORT
F-5
-------
«I
TABLE F-3
Analysis of Samples Collected
From the Siogle looculated Lane Duriag Tett Operatioos
Concentratioas la Bf/f (ppb)
« |J«8
g"§ gS*IIie
8-8 8888~|Srf
« «- «» « **
I II I
8 88 8
ffsffzf
BIIIIrI
|"-SSsIII«5=5
9 * »" » »» » ^ *^ v
1 8 * 8-8SS
8* * 88fi
^*- « ^ ^ * ^
ea- g=g
H
l-l
I'l
'88'
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'IIII I
'IIII I
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|^|§i:IIIIiII
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lifiilillilli
li-888
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I
88
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3 z =
II
88
&*£* sects at S.E.
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SSSSSSSSSSSS
1*1 IIII*£lI
88
II
fl5l|5|
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f'rlilllllili
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f
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I I'l^IIII"!! 78*8888
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I 7gg7gggg
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liiilii
rI~IIII
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g*grr gg88gg 78-8888
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4J
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iiiiiiiiii
?i-IIIIIIII
iiiiiiiiiiili
III
! "
s
iiiiiiiiiiili
=- »£»££
8s!s:r'.s|i
-------
TABLE F-3
Analysis of Samples Collected
From the Single Inoculated Lane During Test Operations
Concentrations in ng/g (ppb)
(continued)
i- ! -
i
x
i
j
J
ii
il
?rrI*H i
H * ^ *» ^ ^« ^
8 ' fi**< i
s s a
«"" ^ = « * * i» * i,
rrll5i *
oK«f99««v« _ _
5 -sgaaZx =. r
I^r=
-f-ifgi
IflHIIlI
* «
I ! i
i i i
HUH
2 J I 1 ! {
S61014/1-FINAL REPORT
F-7
-------
TABLE F-4
Analysis of Samples Collected
From the Multiple Inoculated Lane During Test Operations
Concentrations in nf/f (ppb)
5sy g=|
*
1-1 l£f rS
f*<& <
t ** ** ». «. «^
9 S 9 S 3 X
IIII I
IIII I
m
ii
i
i
~~«
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IIII I * "r ^II-c~Igg
:IIII2I f==?mi25»!f
ins t * ~ -t in
Lfsflll £££s|!assf.s»s
z
ii
II
II
ill
I I l-lrlill
Pi iiiillii
fllllll
IIII' I
iiflii
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'iiii^i
rii
II!IIIIIII
'III* III
lillllllll
'III* III
ii
ill !§!!!!!!{!!! Ilifiii iiiiiiiiiiiii
ii! iii-i-iijiii liiiiii 'i'iiiiiiii
i1!
_ .- imr «* w ^ ^* «
i ii « Mi
; 1115=5*55555;
III IrII IIIIIIII
[Ii liiiflilllil illlll! ifiMiiiiini
' S "X 7577 r r zzM«rr r M « « < < < i ««KXMM«^
If
g<«l«tf
lipIIIIIili lilllil
Migggii -IIIIII
I!
IIIIII
! nil!
8 Ml'I tl
n.uliif!
..I .
^'?y«N.
-------
TABLE F-4
Analysis of Samples Collected
From the Multiple Inoculated Lane During Test Operations
Concentrations in ng/g (ppb)
(continued)
i *
i!
!i
i
?
j i
J
il
* ^ ^ *> j
i Ig s
ggg
I'll!
irl|ll|||
Hllliiil
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lillifli!
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iiiilgl
i «
I «
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w^AwAAM v
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I *
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irrfr~l I r
llrlll! I i
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a £
i *
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i i
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11.
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ll, I
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3
S610U/1-FINAL REPORT
F-9
-------
TABLE F-S
Soluble Nitrate Concentrations in the Pilot Test Area
(I/kf)
Treataent
lane
Control 1
2
3
4
Naan
Inorganic 1
2
3
4
Haw
Inoculated 1
2
3
4
Maan
Multiple 1
Inoculated 2
3
4
Keen
January 26
.........._..
3.6
(.3
3.9
6.3
5.0
6.3
6.9
S.9
7.4
6.6
10.1
14.5
5.2
16.4
11.7
24.1
12.5
S.I
1.7
12.2
February 1
1.
1.
1.
1.
1.
1.7
3.9
9.6
1.6
7.7
8.7
14.6
1.6
1.9
9.7
13.
11.
1.
9.
10.
1 February 16
. . - --
5.0
4.9
2.0
1.5
3.4
1.5
2.3
1.9
<0.1
<1.5
<0.1
1.5
2.9
1.0
0.4
0.9
1.4
1.S
2.3
1.S
March 25
.
0.3
0.3
0.6
0.7
0.5
<0.1
0.3
O.I
0.6
-------
TABLE F-6
Soluble Phosphate Concentrations in the Pilot Test Area
(mg/kg)
Treatment 1
Lane 1 January 26
.» ! ««
1
Control 11 <1.0
2 I <1.0
3 1 <1.0
4 1 <1.0
Hean l <1.0
i
Inorganic 1 ! <1.0
2 1 11.5
3 | 0.0
4 I 0.0
Mean 1 <3.6
I
1
Inoculated 1 | O.O
2 ! <1.0
3 | 0.0
4 ! 0.0
Mean 1 O.O
1
!
Multiple 1 | 0.0
Inoculated 21 O.O
3 | O.Q
4 | O.O
Mean 1 O.O
February 1
^>0 * 1
{Q 1
^0 . 1
^>0 > 1
^0 * 1
588
674
30.0
615
477
665
671
11.6
6.6
339
SOS
IU
3.2
393
466
1 February 16 |
I 0.4 1
1 0.3 1
1 0.1 |
1 0.1 I
i 0.2 i
i j
1 1
1 I
I H9 j
1 57.6 1
1 7.9 I
! 21.9 |
I 59.1 I
i i
i i
1 33.7 1
! 5.0 1
1 13.2 j
I 3.9 j
I 13.9 j
1 1
: i
1 <5.6 1
1 33.8 |
1 8.7 1
1 18.0 j
1 26.5 I
Karch 25 i
0.4
0.1
0.1
0.3
0.2
23.4
23.4
3.3
4.9
13.8
5.5
1.6
2.7
3.1
3.2
13.3
4 J
3.7
S.2
6.8
April 30
{Q J
^0 * 1
^0 1
^0 1
<0.1
7.0
2.2
1.6
1.6
3.1
3.7
1.4
0.9
1.2
1.8
3.2
1.5
1.9
1 2-1
1 2.2
861014/1-FINAL REPORT
F-ll
-------
TABLE F-7
Soluble Ammonium Concentration ia the Pilot Test Area
Treatment
Lane
Control 1
2
3
4
Mean
Inorganic 1
2
3
4
Mean
Inoculated 1
2
3
4
Mean
Multiple 1
Inoculated 2
3
4
Mean
January 26
5.9
9.2
3.6
5.2
6.0
11.4
2.9
31.1
2.5
12.0
1.3
5.0
1.6
4.4
3.1
72.6
63.9
71.9
5.6
S3.5
1 February 1
1 34.1
1 19.7
1 7.6
I 6.9
1 17.1
i
1
1 477
| 274
| 652
| 343
1 437
i
1 569
1 227
! 165
1 24.1
j 246
I
1 40.9
1 171
1 25.3
1 30.6
| 192
February 11
10.
7.
20.
3.
10.
169
55. S
73.4
70.4
92.1
51.7
16.1
SO. 4
19.9
36.3
66.2
46.3
39.3
77.6
S7.1
(torch 25
7.3
12.1
13.5
15.9
12.2
10.4
59.2
119
66.1
63.7
33.7
40.7
49.0
33.9
39.3
62.1
33.1
39.4
45.3
4S.O
April 30
12.2
19.9
14.1
6.7
13.0
72.9
53.4
91.5
46.4
63.5
31.9
29.9
29.5
32.1
30.9
44.5
37.0
39.3
41.5
40.6
S61014/1-FINAL REPORT
F-12
-------
TABLE F-8
Total Organic Carbon Concentrations In the Pilot Test Area
Treatiient 1
Lane 1
Control 1 I
2 I
3 I
4 |
Mean I
I
I
Inorganic 1 I
2 I
3 I
t I
Mean I
1
I
i
Inoculated 1 1
2 !
3 1
4 1
Mean |
i
i
1
Multiple 1 |
Inoculated 2 1
3 1
4 I
Mean 1
January 26
19000
17000
31000
30000
24000
19000
27000
6200
24000
19000
27000
12000
36000
18000
23000
26000
42000
1300
7700
21000
February 16
24000
17000
20000
16000
19000
23000
21000
18000
1SOOO
19000
25000
21000
23000
16000
21000
22000
11000
19000
20000
20000
March 25
21000
29000
14000
18000
22000
20000
19000
18000
1SOOO
18000
20000
21000
21000
17000
20000
20000
23000
17000
26000
22000
April 30
16000
18000
19000
17000
16000
19000
16000
15000
16000
17000
20000
18000
16000
16000
18000
19000
19000
19000
17000
19040
161014/1-FINAL REPORT
F-13
-------
APPENDIX G
Analytical Chemistry Data:
Pilot-Scale Evaluation of Aqueous Biodegradation
-------
APPENDIX G
Analytical Chemistry Data:
Pilot-Scale Evaluation of Aqueous Biodegradation
861014/1-FINAL REPORT G-l
-------
TABLE C-l
Analysis of Samples Collected
From the Bioreactor During Test Operations
Concentrations in ng/g (ppb)
CONTAMINANTS
KETONES
Acacona
2-8utanona
2-Maxancna
SHORT-CHAIN CHLORINATED
HYDROCARBOKS
Chloroforn
Natnylana Chlorida
1,2-Dieh'ioroatnana
1.1,2-Trlchloroathana
1.1.2.2.-Tatraehloroathana
1,1-01chloroathana
frlchloroathana
Tatraehloroathaoa
Bia(2-Chloroathyl)£thar
Haxaehlorooirtadiene
1,1-Oichlereathana
1,2-OSchloroathana
CHLORINATED AROMATIC
HYDROCARBONS
Chlorobanzana
1.2-Oichlorobanzana
l,3-01chlopooanxana
t,4-0ichlorobanzana
1.2.4-Trlehlorobanzana
Haxachlorobanzana
2-Chloronaohthalana
2-OileroeAanol
AMMAT1CS .
Total XylaMs
Ethylbanzana
Styrent
2-flathylnaohthalaM
Mananthrtni
Anthracana
Banzo (a) anthracana
Oibanzo (a.h) anthracana
fluorana
Fluoroantnana
Dlbanxofuran
Banzo (b) fluoranthana
1ft Run I 2nd tun 1 3rd
i i
Initial Final
80L(10)
KM 10)
80LOQ)
BOL(5)
9
BOL,(S)
It
80L(5)
80L(5)
80L(S)
801(5)
KM12)
KM 12)
80L(5)
80L(S)
80L(S)
801(12)
(1)
801(12)
801(12)
(2.4)
801(12)
801(12)
80L(S)
11
801(5)
(1.4)
14
*(0.f)
BOL(12)
80L(12)
80L(12)
(0.7)
80L(12)
80L02)
80L(10)
801(10)
801(10)
BX(S)
9
Bot(S)
80L(S)
BOUS)
801(5)
80t(5)
80L(5)
80L(23)
80L(23)
801(5)
8DL(5)
801(5)
801(23)
801(23)
801(23)
801(23)
801(23)
8X(23)
80L(23)
BOL(S)
801(5)
801(5)
80L(23)
(1-5)
801(23)
801(23)
801(23)
801(23)
801(23)
80L(23)
Initial Final
24
KM 10)
80L(10)
801(5)
11
BOt(S)
t
801(5)
KM5)
801(5)
801(5)
»{9)
KM u)
80L(S)
IOL(S)
80L(5)
80L(14)
(1.5)
80L(14)
801(14)
'(14)
80L(14)
801(14)
801(5)
40
801(5)
(12)
117
(1-3)
KM 14)
80L(14)
(')
(2.T)
80L(14)
80L(lfl)
801(10)
80L(10)
801(5)
|
BOC(5)
80L(5)
BOL(5f
801(5)
KMS)
80L(5)
80L(11)
801(11)
801(5)
80L(5)
Initial
BOL(SO)
BOL(50)
KM 50)
80L(25)
53
790
440
80L(25)
80L(25)
80L(2S)
801(25)
45
80L(11)
801(25)
KM25)
1
1
80t(S)
80L(11)
80L(11)
801(11)
80L(11)
IS
801(11)
80L(11)
80L(5)
8X(S)
101(5}
d)
11
801(11)
801(11)
801(11)
801(11)
80L(2S)
801(11)
801(11)
80L(11)
80L(11)
(8)
8X(11)
801(11)
801(25)
801(25)
KM 25)
(4)
190
(9)
801(11)
801(11)
d)
(5) ! »(4)
801(11) 1 801(11)
KM 23) 1 80L( 14) ! 801(11) 1 801(11)
Run
Final
1
1
t KM 10)
1 KM 10)
1 80LOC)
1
1
*
1
! 83w(5)
1 »5(b)
1 i
-------
TABLE G-l
Analysis of Samples Collected
From the Bioreactor During Test Operations
Concentrations in ng/g (ppb)
(continued)
CONTAMINANTS
3e"ro (k) * uor8'i:Pene
Senrene
Vue->e
&i9ftra'e«e
Chrystne
Pvrene
N-»it 'C$031 onenylaeine
Bcnroic Acic
Acenaonthyieoe
tSTcR
Vinyl Acecatt
Carbon Disul'iae
1st
Initial
_,.... ...i
i
1
301(12) !
SOUS) '
9l!L{5) i
(3.5) 1
3C.C2) 1
*(".C) ;
BOL(12) !
"(3)
301(12} !
i
:
801(10) 1
i
SOUS) 1
un M lun
Final Initial Final
1
BDU23)
SOUS)
8DK5)
SOL (23)
BOL(23)
SOU 23)
BDL(23)
aoLft'S)
301(23}
801(10)
BDL(U) | 801(11} |
801(5} I 801(5) 1
««) 1 801(5) 1
(10)
BDL(*<)
«(5.7)
400(«)
801(70)
*(!.«)
801(10)
801(5) i 831(5)
(-) i
BDL(11) I
14 1
'(10) |
801(55) i
SOL (56) 1
i
1
801(10) 1
1
SOL(S) 1
3rd
Initial
BOL(II)
SOL (25)
801(25)
*(1)
«(J)
'(6)
801(11)
»(S)
'(2)
801(50)
SOL (25)
lun
Fintl
!
1
! 801(11)
i BOL(S)
1 »(1)
! BDU'D
; BOL(K)
*(3)
i BOL(M)
1 «)
i BOL(r)
!
1
1 BOL(IO)
!
! BOL(5)
NOTES: (a) * This mwbtr taken fro* a dilution
(b) Also found in blank. Possible/probable contamination of staple
(c) Above ouantltation ranoe
(t) * Coelutlon of eoMpontntt. total detectable Mount reported
* » Detected but below the netted oua«fif1cat1on HaU (estioated concentration)
80L(x) > BeloM Detection Lialt (EstlMted Detection Halt)
S61014/1-FINAL REPORT
G-3
-------
APPENDIX H
Statistical Analysts of Data
-------
APPENDIX H
Statistical Analysis of Data
The phenanthrene data obtained from the Brio DOP site was analyzed using a three-
factor analysis of variance. The pilot test was designed such that there were three main
sources of variation influencing the concentration of phenanthrene over the sampling
period. These factors were treatment, location in the field and sampling time. There
were four levels of each factor. These were as follows: treatment (control, inorganic,
inoculated and multiple inoculated), field location (four plots per treatment) and sampling
time (day 0, 21, 58, and 94). A total of 64 samples were obtained (4 x 4 x 4). The data
was log transformed prior to analysis in order to normalize the frequency distribution
and to equalize within factor variances.
The analysis of variance table shows the sources of variation and their associated sums
of squares, degrees of freedom, mean-squares, F ratio and P value. In a three factor
design there are three main factors, three first order interactions (treatment x location,
treatment x time and location x time) and one second order interactive (treatment x time
x location). Since the statistical design is in essence an expansion of a randomized
complete block design without replication there is no measure of pure error. In this
case additivity is assumed and the mean square for the second-order interaction is used
for the error term.
This analysis indicates that the main effects: treatment, location, and time, as well as
one first order interaction, location x time were significant at P s .05. Interpretation of
main effects is not meaningful given any significant interactions. Therefore the only
significant effect that can be interpreted in the location x time interaction. In this case
the rate of phenanthrene degradation over time was significantly different between the
different field locations. The lack of significance of the treatment x time interaction
indicates that the rate of phenanthrene degradation over time was not significantly
different between the different treatment options.
861014/1-FINAL REPORT H-l
-------
TABLE H-l
Three Factor Analysis of
Pheaaathrea* Coaccatratloas
DEF VAR: LOGPHEN
Ni
64
MULTIPLE Rt .926
SQUARED MULTIPLE Ri .8
SOURCE SUM-OF-SQUARES
ANALYSIS OF VARIANCE
DF MEAN-SQUARE
F-RATIO
P
TREAT
LOC
TIME
TREAT*
LOC
TREAT*
TIME
LOC*
TIME
ERROR
4.215
7.965
16.212
4.924
4.854
15.289
8.868
3
3
3
9
9
9
27
1.405
2.655
5.404
0.547
0.539
1.699
0.328
4.278
8.O84
16.453
1.666
1.642
5. 172
0.014
O.O01
O.OOO
0. 147
0.153
O.OOO
SYSTAT PROCESSING FINISHED
INPUT STATEMENTS FOR THIS JOB:
OUTPUT««
USE BRI011
CATEGORY TREAT«4, LOC-4, TIME-4
MODEL LOGPHEN«CONSTANT+TREAT-H_OC+TIME+TREAT*LOC+TREAT»TIME+LOC*TIME
ESTIMATE
61014/1-FINAL REPORT
H-2
-------
APPENDIX I
Laboratory Quality Assurance/Quality Control Data
-------
APPENDIX I
Quality Assurance/Quality Control Data
Seventy-two (72) soil samples were received between 1/30/87 and 5/1/87 for the analysis
or volatile organics by purge and trap GC/MS; acid and base/neutral extractables by
GC/MS; TOC; soluble ammonium; nitrate; and phosphate. Where appropriate, standard
EPA methods were utilized. Table 1-1 summarizes the methods used.
Due to the sample volume during January, the first 18 soil samples were sent to Califor-
nia Analytical Labs, Inc. (CAL). As a subcontractor, CAL used the EPA-CLP Program
QA/QC requirements for the analysis of this set of samples which are the sample
protocols that Ecova used on the following three sets of samples. A description of the
QA/QC for these samples follows. In most cases, the QA/QC falls within the established
QA/QC ranges established by the U.S. EPA contract lab program. Samples with QA/QC
outside these limits are marked with an asterisk and are believed to be matrix problems
due to the large levels of organics present.
GC/MS
Prior to the analysis of samples or standards, the GC/MS system was tuned and calibra-
ted according to the instrument manufacturer's instructions. The tune was verified by
comparing the spectra of either BFB (bromofluorobenzene) for volatiles or DFTPP
(decafluorotriphenyl phosphine) for acid/base neutral extractabies with the known
fragmentation pattern for these compounds. EPA-specified criteria were met every 12
hours prior to any further analysis.
An initial calibration was performed on the instrument consisting of five concentration
levels of each compound of interest. Six compounds were used as calibration check
compounds (CCC) as described in the EPA Contract Laboratory Program. The maximum
percent difference of the relative response factor for each of these compounds is 30
percent for acceptability of the multipoint. Every 12 hours, after the BFB or DFTPP
tune, the SO ppb standard was analyzed and the CCC compounds were checked versus the
multipoint calibration. The maximum percent difference of the relative response factor
for each of these compounds is 25 percent. This criterion was met prior to any sample
analysis.
Reagent blanks (laboratory water) were analyzed directly following the shift standards for
volatiles and once every 20 extractions for the acid/base neutral samples. In all cases,
these blanks contained no compounds above the EPA acceptable limits established for the
Contract Laboratory Program.
Surrogate compounds were added to all samples prior to extraction or purge and trap
analysis. These surrogates are monitored in every sample and checked for recovery.
Tables 1-2 (Time 0 CAL Data), 1-3 (Time 21 days), 1-4 (Time 58 days), and 1-5 (Time 94
days), list the results of the surrogate recoveries for all samples in this study. An
asterisk (*) marks the. recoveries outside the contract required QC limits. Due to the
high concentration of organics in these samples, many of the extracts required dilutions
and, therefore, the surrogate levels were diluted below the limits of quantitation.
Duplicate matrix spikes were analyzed for both volatiles and semivolatile fractions every
20 samples. Five compounds from each compound class (VOA, acid, or base neutral) were
861014/1-FINAL REPORT M
-------
spiked into the soil prior to purge and trap or extraction and measured for recovery and
precision of duplicates. An asterisk marks the recoveries that fall outside the established
QC ranges. These ranges are advisory levels only and in no way invalidate the data.
Tables 1-6 (Time 0 CAL Data), 1-7 (Time 21 days), 1-8 (Time 58 Days), and 1-9 (Time
94 Days), Hst the results of these matrix spikes. The acid/base neutral spike sample
from time 21 days (Table 1-7) required a 5:1 dilution and therefore contained several
compounds outside of the QC limits.
TABLE 1-1
Analytical Methods Summary
Analysis Method
Volatile Organics EPA 624/8240
Acid/Base Neutral EPA 625/8270
Nitrogen as Ammonium 417 E, Standard Methods for the Examination of
Water and Wastewater. 15th Edition
Nitrate 418 C, Standard Methods for the Examination of
Water and Waste water. 15th Edition
Phosphate 424 E, Standard Methods for the Examination of
Water and Wastewater. 15the Edition
8610H/1-FINAL REPORT 1-2
-------
TABLE 1-2
Tim* O: Surrogate Results
28 7
5
i
§ -
6% ^
« HI
x* -
O t*t t^ ^
u S o
X *.
» W M
sss
|§§
idl
ill
^A
V
§
§
i 2
o «
H
861014/1-FINAL REPORT
1-3
-------
TABLE 1-2
Tine O: Surrogate Results
(coitliued)
i8 ?
-» o
am K
e
5
i
s
i
*-
«
i
i
i
^ *»
S
SSSSSIZS
scssfcsii
SoSSSSSS
S A
if S
= SSS4SSSS
«««
%«
2 Sf C
5 Sv =
i ts s
S> 8J ""
5 -
SI
-«.
r SSS8S8SS
sft S
s r
= | 5
§- £
X C
s« =
0
* §
sssuxes
iss lisas
f*SIf?28
. Jsfsf
S65
sss
M V» VI
888
lil
8
8
i
s -
§ *
8 i
It =
ii
S 9
M1014/1-FIN.AL REPORT
1-4
-------
TABLE 1-3
Time 1 (21 Days): Surrogate Results
5««;«5«5S"2SSSS"22SE~2S3SS25XC!I2ft2S"S2
8 -
SS H
861014/l-FINAL REPORT
1-5
-------
TABLE 1-3
Time I (21 Days): Surrogate Results
(continued)
861014/l-FINAL REPORT
1-6
-------
TABLE 1-4
Time 2 (58 Days): Surrogate Results
V Z C
S i
::
155
S610U/1-FINAL REPORT
1-7
-------
TABLE 1-5
Tin* 3 (94 Days): Surrofite Results
s:
S3
61014/l.FINAL REPORT
-------
TABLE 1-6
Time O: Soil Matrix Spike/Matrix Spike Duplicate Recovery
8
5.
!
*
S8882
ft
88888
Hi
.
Trlch
Cltlor
t*lum«
Mniww
'.oo^o5! a-
S3SSSC
'<*i
^,2 M
lls 15
mom
NIM
(ill
!-*!!
2222
JJJJ
jTu
III!
!
I$£i£8
Iisst"
-* i « e«i-«
****
i
i
!
i
III
iV
te ^ ^ ^
D
5555
861014/1-FINAL REPORT
1-9
-------
TABLE 1-6
Tine O: Soil Matrix Spike/Matrix Spike Duplicate Recovery
(contiaued)
ss«S5S £«ss= 55355
SUSRSR i
8
g 5
i *
X IW
w
u
^ *
P
Ml ^
f!
^ **,*"*" 2 2 2 ° °
gjjjjgj *5*-*
o « « « «
at
3 X w e
"i "
liiiii
. { «
? I If
Hi i!
;««.««
~ I
1!!!
i
Wit
Kill!
StSoSV
I
1331
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Tfu
^s?^
UI.I.
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161014/l-FINAL REPORT
MO
-------
TABLE 1-7
Time 1 (21 Days): Soil Volatile Matrix Spike/
Matrix Spike Duplicate Recovery
Customer *
EAS #
Matrix
Method
CH0900
202-62 MS and MSD
Soil
EPA 624 GC/MS Purge and Trap
Date Sampled: 2-17-87
Compound
1,1-Dichloroethene
Trichloroethene
Benzene
Toluene
Chlorobcnzcnc
SPIKE
ADDED
(ug/Kg)
49
49
49
41
48
SAMPLE
CONC.
(ug/Kg)
0
0
0
1
0
MS
CONC.
(uf/Kg)
58
45
44
42
44
MS
S
REC*
118
92
90
100
92
QC
LIMITS
REC
59-172
62-137
66-142
59-139
60-133
Compound
1,1-Dichloroethcne
Trichloroethene
Benzene
Toluene
Chlorobenzcne
SPIKE
ADDED
(ug/Kg)
56
44
44
42
43
MSD
CONC.
(ug/Kg)
114
90
90
100
90
MSD
REC*
118
92
90
100
92
RPD*
3
2
0
0
2
QC LIMITS
RPD REC
22
24
21
21
21
59-172
62-137
66-142
59-139
60-133
* Column to be used to flag recovery and RPD (Response % Difference) values with an
asterisk.
' Values outside of QC limits.
RPD: JL out of _5_ outside limits.
Spike Recovery: 0 out of 10 outside limits.
46/1014.SPK
S61014/I-FINAL REPORT
Ml
-------
TABLE 1-7
Time 1 (21 Days): Soil Volatile Matrix Spike/
Matrix Spike Duplicate Recovery
(continued)
Customer
EAS *
Matrix
Level
Compound
Phenol
2-Chlorophenol
1,4-Dichlorobenzene
N-Nitroso-di-n-
Propylaminc
1,2,4-Trichloro-
benzene
4-Chloro-3-Mcthyl-
phenol
Acenaphthene
4-Nitrophenol
2,4-Dinitrotoluene
Pentachlorophenol
Pyrene
Compound
Phenol
2-Chlorophenol
1,4-Dichlorobenzene
N-Nitroso-di-n-
Propylamine
1,2,4-Trichloro-
benzene
4-Chloro-3-Mcthyl-
phenol
Acenaphthene
4-Nitrophcnol
2,4'Dinitrotoluene
Pentachlorophenol
Pyrene
CH0902
202-18 MS and MSD
Soil
Low
Date Sampled 2/17/87
SPIKE
ADDED
(ng)
258000
242000
103000
109000
108000
210000
127000
N/A
98000
210000
105000
SAMPLE MS
CONC. CONC.
On/Kg) (ut/Kg)
0
0
0
0
0
N/A
0
0
8
MSD CONC
IN EXTRACT MSD
(ug/Kg) REC *
53.50
41.45
9.8
27.30
17.40
66.3
0
N/A
58.3
132.0
61.75
21*
17*
9.5*
26*
16*
31.5
0*
N/A
60
63
60
52.75
40.0
9.85
27.55
16.0
72.6
0
N/A
63.2
143
95
MSH
tfEC*
20*
16*
9.5*
25*
14*
34
0*
N/A
65
68
90
MS
%
REC*
20*
16*
9.5*
25*
14*
34
0*
N/A
65
68
90
QC
LIMITS
REC
26-90
25-102
28-104
41-126
38-107
26-103
31-137
11-114
28-89
17-109
35-142
RPD*
3
6
0
13
8
100*
N/A
8
8
20
QC LIMITS
RPD REC
35
50
27
38
23
33
19
50
47
47
36
26-90
25-102
28-104
41-126
38-107
26-103
31-137
11-114
28-89
17-109
35-142
Values outside QC limits.
* Column to be used to flag recovery and RPD values with an asterisk.
RPD: I out of 10 outside limits.
Spike Recovery: J2 out of 22 outside limits.
Comments: Samples were analyzed at X5 dilution.
46/1014-SPK.SV
S61014/1-FINAL REPORT
M2
-------
TABLE I-S
Time 2 (58 Days): Soil Volatile Matrix Spike/
Matrix Spike Duplicate Recovery
Customer
EAS*
Matrix
Method
CH0933
227-17 MS and MSO
Soil
EPA 624 GC/MS Purge and Trip
Date Sampled: 3-26-87
Compound
1,1-DichIoroethcne
Trichloroethene
Benzene
Toluene
Chlorobenzene
SPIKE
ADDED
(ug/Kg)
49
49
49
41
48
SAMPLE
CONC.
(ug/Kg)
2
0
0
16
3
MS
CONC.
(«f/Kg)
SI
58
46
54
55
MS
%
REC*
100
118
94
93
108
QC
LIMITS
REC
59-172
62-137
66-142
59-139
60-133
Compound
1,1-Dichlorocthcnc
Trichloroethene
Benzene
Toluene
Chlorobenzene
SPIKE
ADDED
(ug/Kg)
49
49
49
41
48
MSD
CONC.
(ug/Kg)
52
59
47
54
55
MSD
%
REC*
102
120
96
93
108
RPD
2
2
2
0
0
QC LIMITS
RPD REC
22
24
21
21
21
59-172
62-137
66-142
59-139
60-133
* Column to be used to flag recovery and RPD (Response % Difference) values with an
asterisk.
* Values outside of QC limits.
RPD: 0 out of 5 outside limits.
Spike Recovery: 0 out of 10 outside limits.
46/1014.SPK
S61014/1-FINAL REPORT
1-13
-------
TABLE 1-8
Time 2 (58 Days): Water Volatile Matrix Spike/
Matrix Spike Duplicate Recovery
(continued)
Date: 4-9-17 Project No. §61014
WATER VOLATILE MATRIX SPIKE/MATRIX SPIKE DUPLICATE RECOVERY
Customer #
EAS «
Matrix
Level
Compound
Phenol
2-Chlorophenol
1,4-DichIorobcnzcnc
N-Nitroso-di-n-
Propyltminc
1,2,4-Trichloro-
benzene
4-Chloro-3-Mcthyl-
phenol
Aeenaphthene
4-Nitrophenol
2,4-Dinitrotoluene
Pentachlorophenol
Pyrene
Compound
Phenol
2-Chlorophcnol
1,4-Dichlorobenzenc
N-Nitroso-di-n-
Propyiamine
1.2,4-Trichloro-
benzene
4-Chloro-3-Methyl-
phenol
Aeenaphthene
4-Nitrophenol
2,4-Dinitrotolucnc
Peataehlorophenol
Pyreae
CH0933 Date Sampled 3-15-87
227-17 MS and MSD
Soil
Low
SPIKE
ADDED
(ng)
258000
242000
103000
109000
108000
210000
127000
N/A
98000
210000
10SOOO
SAMPLE
CONC.
(ug/Kg)
0
0
0
0
0
0
0
0
7
MSD CONC
IN EXTRACT MSD %
(ug/Kg) REC«
123
97
23
56
42
130
92
N/A
71
146
114
48
40
22*
51
39
62
72
N/A
72
69
102
MS
CONC.
(«g/Kg)
140
104
22
67
37
123
81
N/A
70
137
68
MS*
REC*
54
43
21*
61
34*
59
64
N/A
71
65
58
MS
%
REC*
54
43
21*
61
34'
59
64
N/A
71
65
58
RPD «
12
7
5
18
14
5
12
N/A
2
6
78*
QC
LIMITS
REC
26-90
25-102
28-104
41-126
38-107
26-103
31-137
11-114
28-89
17-109
35-142
QC LIMITS
RPD REC
35
SO
27
38
23
33
19
50
47
47
36
26-90
25-102
28-104
41-126
38-107
26-103
31-137
11-114
28-89
17-109
35-142
RPD: 1 out of 10 outside limits.
Spike Recovery: 3 out of 20 outside limits.
Values outside QC limits.
« Column to be used to flag recovery and RPD values with an asterisk.
Comments: Matrix Spike Duplicate contained higher levels of most of the PNAs and
therefore the RPD for pyrene was out of limits. All other recoveries are
very close.
161014/1-FINAL REPORT
1-14
-------
TABLE 1-9
Time 3 (94 Days): Soil Semivolatile Matrix Spike/
Matrix Spike Duplicate Recovery
Customer #
EAS *
Matrix
Level
Compound
Phenol
2-Chlorophcnol
1,4-Dichlorobcnzene
N-Nitroso-di-n-
Propylamine
1,2,4-Trichloro-
benzene
4-ChIoro-3-Methyl-
phenol
Acenaphthene
4-Nitrophenol
2,4-Dinitrotoiuene
Pen tachlorophenol
Pyrene
CH0357
247-09 MS and MSD
Soil
Low
Date Sampled 4-30-87
SPIKE
ADDED
(ng)
258000
242000
103000
109000
108000
210000
127000
N/A
98000
210000
105000
SAMPLE
CONC.
(ug/Kg)
0
0
0
0
0
0
0
N/A
0
0
8
MS
CONC.
(ug/Kg)
161
130
54
106
66
143
94
N/A
66
121
77
MS
%
REC*
62
54
52
97
61
68
74
N/A
67
58
66
QC
LIMITS
REC
26-90
25-102
28-104
41-126
38-107
26-103
31-137
11-114
28-89
17-109
35-142
MSD CONC
IN EXTRACT MSD %
(ug/K«) REC «
163
135
63
109
73
63
56
61
100
67
MS*
REC«
62
54
52
97
61
RPD *
2
4
16
35
50
27
38
23
Compound
Phenol
2-Chlorophenol
1,4-Dichlorobenzene
N-Nitroso-di-n-
Propylamine
1,2,4-Trichloro-
benzene
4-Chloro-3-Methyl-
phenol
Acenaphthene
4-Nitrophenol
2,4-Dinitrotoluene
Pentachlorophenol
Pyrene
* Values outside QC limits.
* Column to be used to flag recovery and RPD values with an asterisk.
RPD: 0 out of 10 outside limits.
Spike Recovery: 0 out of 20 outside limits.
QC LIMITS
RPD REC
26-90
25-102
28-104
41-126
38-107
152
105
N/A
72
131
76
72
82
N/A
73
62
65
68
74
N/A
67
58
66
6
10
N/A
9
7
2
33
19
50
47
47
36
26-103
31-137
11-114
28-89
17-109
35-142
861014/1-FINAL REPORT
1-15
-------
TABLE 1-9
Tine 3 (94 Days): Water Volatile Matrix Spike/
Matrix Spike Duplicate Recovery
(continued)
Customer
EAS *
Matrix
Method
CH0357
247-09
Soil
EPA 8240 GC/MS Purge and Trap
Date Analyzed 05-04-87
Compound
1,1-Dichloroethene
Trichloroethene
Benzene
Toluene
Chlorobenzene
SPIKE
ADDED
(ug/Kg)
49
49
49
41
48
SAMPLE MS
CONC. CONC.
(ug/Kg) (ug/Kg)
0
0
0
26
2
SO
48
42
64
44
MS
%
REC *
98
98
86
93
88
QC
LIMITS
REC
59-172
62-137
66-142
59-139
60-133
Compound
1,1-Dichlorocthene
Trichloroethene
Benzene
Toluene
Chlorobenzene
SPIKE
ADDED
(ug/Kg)
49
49
49
41
48
MSD
CONC.
(ug/Kg)
50
46
40
60
44
MSD
REC * RPD *
98
94
82
83
88
0
4
5
11
0
QC LIMITS
RPD REC
22
24
21
21
21
59-172
62-137
66-142
59-139
60-133
* Column to be used to flag recovery and RPD (relative percent difference) values
with an asterisk.
Values outside of QC limits.
RPD: 0 out of 5 outside limits.
Spike Recovery: 0 out of 10 outside limits.
Comments: Recoveries calculated on a wet weight basis, directly from quantitation
report.
42/861014.MSD
161014/1-FINAL REPORT
1-16
-------
APPENDIX J
Microbiological Methods
-------
APPENDIX J
Microbiological Methods
J.I Enumeration of Aerobic Heterotroohic Microorganisms
One-gram aliquots of soil were serially diluted through 9-mI aliquots of Bushnetl-
Haas medium (Table J-l), and triplicate 0.1-ml aliquots of the appropriate dilutions
were spread on plates of Bushnell-Haas medium plus 0.1% weight per volume (w/v)
peptone and 0.1% (w/v) yeast extract. Plates were incubated at 25°C for seven
days. Colony forming units (CFU) were then enumerated, and distinct morphological
types noted.
TABLE J-l
Composition of Bushnell-Haas Medium
MgSO4.7H2O 0.20g
CaCl2.2H2O 0.02g
KH2PO4 l.OOg
K2HPO4 l.OOg
NH4NC>3 l.OOg
FeCli 0.05g
Purified agar IS.OOg
Distilled water 1,000 ml
pH 7.0
J.2 Laboratory Microcosm Evaluation Technique
The objective of the laboratory evaluation studies was to determine if the diverse
microbial population in Pit O could degrade the organic constituents present. To
accomplish this, aerobic microorganisms were enriched from the surface to 5 ft and
the 5 to 10 ft composite samples. No enrichment cultures were performed with the
10 to 15 ft composite samples.
Fifteen grams of soil were added to SO ml of Bushnell-Haas medium. Inoculated
flasks were incubated for 28 days at 2S°C on a rotary shaker. Microbial growth
was assessed by both microscopic examination and by enumerating the numbers of
microorganisms present in enrichment cultures. Numbers of aerobic heterotrophic
microorganisms were determined on days 0, 7, 14, 21, and 28.
J.3 Radiotracer Techniques
Radiotracer analysis confirmed biological activity. Soil samples from the treatment
facility were spiked with 14C-labelled compounds and the biological mineralization of
these compounds-to 14C-labelled carbon dioxide was measured.
Two radio-labelled compounds were used to monitor biological activity within the
pilot test area:
861014/1-FINAL REPORT J-l
-------
Glucose. The activity of heterotrophic microorganisms was determined by
measuring the mineralization of 14C-labelled glucose to 14C-labelled carbon
dioxide.
Phenanthrene. The baseline chemical characterization identified phenanthrene
as the major semi-volatile contaminant in Pit O. The phenanthrene biodegra-
dation potential within the pilot test area was monitored by measuring the
mineralization of 14C-labelled phenanthrene to 14C-labclled carbon dioxide.
One-quarter gram aliquots of soil were placed in the incubation vessel (Figure
J-l). Five ml of distilled water containing the required radio-labelled com-
pound were added to each sample. After the required incubation period (24
hours at 25°C for 14C-glucose, and 7 days at 25°C for 14C-phenanthrene), the
reaction was terminated by the addition of 0.05 ml of concentrated sulfuric
acid. The acid terminated biological activity and drove carbon dioxide (CO2>
out of solution. The released 14C-Iabelled C(>2 was trapped on a 20-mm by
50-mm Whatman *1 filter paper wick containing 0.5 ml of phenethylamine, a
CC>2 trapping agent. To maximize 14CC>2 adsorption by the wick, the incuba-
tion vessel was gently shaken overnight in a 35°C waterbath. The wick was
subsequently removed and transferred to a scintillation vial containing 5 ml of
Beckman Ready-Solv CP scintillation cocktail. 14CO2 was measured by liquid
scintillation counting. All counts were corrected for the trapping efficiency of
14CC>2 by the phenethylamine wick system (100%), wick self-adsorption (xl.l),
and counting efficiency by the Beckman H number method. '4C-labelled
compounds present in the aqueous phase were determined by counting a 0.1-ml
aliquot of that phase. All experiments were run in triplicate. The experiments
included sulfuric acid-killed controls to monitor non-biological effects.
861014/1-FINAL REPORT J-2
-------
20mm. X 50mm. WHATMAN *1
FILTER PAPER WICK SATURATED
WITH 0.5ml. OF PHENETHYLAMINE,
(A CO2 TRAPPING AGENT)
20mm. X 150mm.
PYREX TEST TUBE
RUBBER STOPPER
POLYPROPYLENE CUP
ASSEMBLY FOR
SUPPORTING THE
FILTER PAPER WICK
SAMPLE PLUS
RAOIOTRACER
FIGURE J-l
lacubatioa Vessel
for Radlotracer Studies
-«»*
E C 0 V A
INCUBATION VESSEL
FOR RAOiRACER STUDIES
S610U/1-FINAL REPORT
J-3
-------
APPENDIX K
Microbiology Data
-------
APPENDIX K
Microbiology Data
861014/1-FINAL REPORT K-l
-------
TABLE K-l
Numbers of Aerobic Heterotrophic Microorganisms
Present ir Enrichment Cultures
Time (Days)
Numbers of Microorganisms (CPUs/ml slurry)
SAHFLE 1
CK0132 iHA
CHOli: BrtPV
CHOI 32 BHE!
CH0132 EH
LH0133 6HA
CH0133 BHFl
CHOI 33 BHcI
CHC133 6h
DW135 BHA
CHOI 35 Brtr*
CHOS3S IHEI
CH0135 BH
CHOI 36 BHA
CH0136 BHFt
CHCi3o BHEI
CK0136 BH
CHOI 3? iHA
CH0139 BriPY
CHOI 39 IHEI
CHOI 31 BH
CHOUO BHA
CHOI 40 BHft
CH0140 BHEI
CH0140 Bri
I
4.59110*
4.5*110*
5.47116'
4.5'illO*
4.91I10r
4.91110*
4.63110'
4.9IIIO*
2.42110*
2.42110*
6.27I1C'
2.42110*
1. 67110*
1.87110*
5.471107
1.87I101
1.26110*
1. -24110*
6.J7UO*
l.2iI10»
I.59MO*
1.59X10*
S.tCUO'
I.KI10*
0
so
ND
MI
B.9iIlO»
Ku
KD
NO
3.21I10»
ND
NO
ND
2. OHIO*
KD
ND
M
4.04110*
Nu
NO
KC
4.UI10*
ND
ND
N5
5.2*I1(>»
ND
7
7 id
2.07U01 2.10I101
2.2SilOT 1.5MIO*
1.23110' 1.42110'
4.12HO» 4. MHO8
4.33110' 2.06110'
3.13110' 5.77110*
B.07110* 5.13I109
3.BSl'lu» l.l4Ild9
6.33110* 4.14I101
6.30Uu» 1. 71110*
.07110* 2.08110*
.54110' 1.20110*
.23110* A.AillO'
.30110* 4.11110'
.30110' 4.51110*
.83110' 2.21110*
1.44110* 2.20110*
7. 50110* 2.431lOi
3.67110" i.itXIO*
1. 12110* 2.UIIO'
7.47110' 1.50110*
1.35110* 1.44I101
4.10110' 5.29110*
3.93X10* 4.04110*
14
T id
4.17110* 7.511lOa
1.14110* 4.241107
1.1U10' 1.B1HO*
5.77110' 2.04110'
i.001101 2.00110'
2.92I1C* l.fillO*
4.83110* 7.i4I10*
9.10110* 1.7U10*
1.281 10* 4.93110*
3.13110* S.B0116*
2.871)0' 4.16110*
1.14110' 9.I7UO*
4.97110s 5.15I101
3.97110* 9.41110'
7.93110* 1.B9IIO*
1.B7HO' 2.27110*
1.271104 9.54110s
4.10110* 1.00110'
3.37110' 3.21110*
9.67110' 8.14110*
1.08110* 1.B4110*
1.79110* 1. 50110*
9.73110* 1.82110*
3.40110* 2.MI10*
21
r id
9.33110* 7.02110'
7.73110* 4.73110'
3.47110* 3.21IIG*
6.63110' 7.51110-
3.33110' 1.15110'
1.93110* 2.30110'
4.70110* 7.00110*
5.03110* 9.50110*
7.53110s 1.UI10*
1.17110* 2.15110'
9.40110' 5.71110*
7.901IC* 3.00110*
8.87110s 5.51110'
1.90X10* 2.08110*
3.83X10* 4.04X10*
1.57X10' 1.67X10*
1.UI104 1.57X10*
1.66X10* 1.55X10'
1.40110' M5IIO*
5.17110' 2.21X10'
8.10X10* 1.18X10*
2.25X10* 9. 171 10*
f.77110* S.77110"
3.37X10* 2.52X10*
26
r sd
B.47I10* 1.01110*
3.67110* 5.34110'
1.04II&* 1. 53UO*
3.97110' 4.731H1*
4.00110' 5.29I1&1
2.44110* 7.97110'
1.89X10* 3.21110*
5.63X10* 5.71110*
7.27X10* 2.76110*
1.39110* 2.0BI10'
6.63HO* 5. 13110*
7.53110* 1.77110*
5.53110* 1.721!
1.29110* 1.23110
1.77X10* 1.14110*
1.27110' 1.27X10*
7.30X10* 1.30110'
1.17X10' 1.53110*
8.17110* 1.10X10*
9.23110* 2.45110*
3.23110* 4.04X10'
1.34110* 2.40X10'
2.04X10* 2.23/10*
1.27X10* 5.51110*
BH - Bushnell-Haas Medium
BHP** Bushnell-Haas + Peptone/Yeast Extract
BHEI" Bushnell-Haas + Ecova Inoculum
BHA » Bushnell-Haas + Azide
x - Mean
s.d. « Standard deviation
861014/1-FINAL REPORT
K.-2
-------
a-"
**
8
m
»
>
T
3
t*
TABLE K-2
Numbers of Aerobic Heterotrophic
Microorganisms in Pilot Test Treatment Lanes
s
» tO H"* U"l c*<4 «> f>V
£ -S -
r*22 22SS2 22222 2222
M«»M«»X « -J rt »*« «> M in M
s
S _
r «f>: »
3
.f< s 3 s
I1 h i ill! i i! i III 2
861014/1-FINAL REPORT K-3
-------
APPENDIX L
Operational Sampling Locations
-------
APPENDIX L
Operational Sampling Locations
861014/1-FINAL REPORT L-l
-------
/
/
4
s
3
\
/
2
\
/
1
\
C N 1 M
CH1024
CH1025
CH102S
CH1027
CH1020
CH1021
CH1022
CH1023
CH1016
CH1017
CH101S
CH1010
CH1012
CHt013
CH1014
CH101S
CH1040
CH1041
CH1042
CH1043
CH103*
CH1037
CH1038
CH1038
CH1032
CH10S3
CH1034
CH103S
CH1028
CH1020
CH1030
CH1031
CH1088
CH10S7
CH1088
CHIOS*
CH1082
CH1083
CH1054
CH10SS
CH104S
CH1040
OHIO SO
CH1081
CH1044
CH104S
CH104S
CH1047
CH1072
CH1073
CH1074
CH107S
CH10SS
CHIOS*
CH1070
CH1071
CH10S4
CH10SS
CH10SS
CH10S7
CHioeo
CH1081
CH10S2
CH10S3
C N I M
29
JL
Dp
22
15
8
FIELD BLANKS
CH1076
CH1077
CH1078
1
POST
EXPLANATION
C CONTROL
N -NUTRIENT ADJUSTED
MQTE8-
M
1. REMOVE 3 RANDOM SAMPLES PROM EACH
QUADRANT AND COMPOSITE.
2. SAMPLES SHOULD BE REMOVED PROM THE
TREATMENT ZONE (TOP «')
3. SAMPLF.S SHOULD BE REMOVED FROM THE
CENTRAL 10- OF EACH TREATMENT LANE
INOCULATED
MULTIPLE INOCULATED
.~«uu;
...««
.«««»§
>-<«
F r. n v A
MI014/LFINAL REPORT L-2
RIO SITE TASK FORCE
RIO PROCESS AREA
SAMPLING PLAN
JANUARY 27. 1967
-------
tl
M
z
90
m
»
O
so
H
SAMPLE LOCATION SUMMARY
MONSANTO
PROJECT NUMBER
1014
CONDITIONS
N\A
PIT/BORINO no.
N\A
LOCATION
NIO REPINERY SITE ENCLOSURE
SAMPLE DBBION «T ROSSNLAAKSO
DATE
1\1«\«7
TIME
SAMPLE
NUMBER
CHI01*
CHI01*
CRioao
CH1014
CH101B
CHIOS*
CHlOSt
CN1040
CH10«4
CH10M
DErn
o- -
o- -
o- . ,.
o- -
o- -
o- -
o- -
-
o- -
-
SAMPLE DEST. /CONTAINER SIZE
PUTS CHEN MtO OTHER,
1
I
I
I
I
I
I
I
I
I
SAMPLE
METHOD
S
'
CREST
NO.
SAMP
TYPE
S
*
S
S
S
S
PRES-
ERVAT
CHILL
CNILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TIME
to
DATS
10
DAYS
10
DAYS
to
DAYS
1O
DAYS
10
DAYS
10
DAY*
10
DAYS
10
DAYS
10
DAYS
TEST DESCRIPTION \ NOTES
TOTAL OROANIC CARSON.
ASE NEUTRAL ACID EXTRACTABLES
TOTAL ORGANIC CARBON.
ASE NEUTRAL ACtO CXTRACTABLES
TOTAL OROANIC CARBON.
ASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
ASE NEUTRAL ACID CXTRACTASLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
ASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
ASE NEUTRAL ACID EXTRACTABLES
AINKH DESCRIPTION!
- I ! A«fc J«« (4 It)
I ! Jar 14 It)
- t/a ! Jar
- 1 Pt *» J«r
- |/t Pt A«k Jar
- VOA Vial
- Cora
- Mhlrl Pack
- I qt A»b Jar
(t It)
(4SOM1)
SAMPLE NETHODt
Dlat«rk*d
On41at«rb«d
Drill
Baekhoa
Troml
Ballar
T
Hand Cera
S Split Spoon
10 Drab
11 CoMpoalta
12 Hand Auger
SAMPLE TYPE)
S-Soll
N-Mat«r
0-011
X-Othcr (dcacrlbal
rev 1/I/rr
(SAMPLE.rOR)
-------
SAMPLE LOCATION SUMMARY
at
PROJECT NAM
HONSANTO
PROJECT NUMBER
1014
eomtriom
UNA
r
M
M
«
o
jo
H
PIT/BORINO HO. N\A
tOCATION
BRIO REPINERT «IT« ENCLOSURE
SAMPLE DESION »T
ROSSXLAAESO
BATE l\m«7
Tim
SAMPLE
NUMBER
CN1O4B
CHIOS*
CHI 0(0
CN10CB
CNIOC4
CNIOTt
CNIOTC
DEPTH
0- - i"
o- -
o« -
-
e- .
o- -
0" -
SAMTtE
PUTS
DECT. /CONTAINER SIZE
CNEH HMO OTHER
I
I
I
I
I
I
I
SAMPLE
HtTROO
S
S
'
CHEST
NO.
SAMP
TTPE
S
S
S
S
S
S
PRES-
CRVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
BOLD
TINE
10
OATS
10
OATS
10
OATS
1O
DATS
10
DATS
10
OATS
10
DATS
TEST DESCRIPTION \ NOTES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL ORttARIC CARBON.
BASE NEOTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACIO EXTRACTABLES
TOTAL OROANIC CARSON.
BASE NEUTRAL ACID EXTRACTABLES
SAMPLE CONTAINER DESCRIPTION I
t 0*1 Aafc Jmg ( It)
1 ! Jar (4 It)
1/1 9ml Jar
I Pt A*» Jar
l/fl Pt » Jar
VOA Vial
Car*
Whirl Pack
I Qt Auk Jar («IO*I)
|l It)
<«SO«1»
NBTHODi
Dlat«rb*4
OMlatarkad
Drill
Baekhaa
Trowal
Ballar
Hand Cora
Split Spoon
1O Orak
11 Conpoalta
IS Rand Avgar
SAMPLE TTPEl
S-Sall
W-Matar
O-OI1
X-Othar |4«aerlba)
-------
SAMPLE LOCATION SUMMARY
P«g« 3 of B
PROJECT NAME MONSANTO
PROJECT NUMBER MIOI4
CONDITIONS
N\*
f*
90
M
O
90
H
PIT/BORING) NO. N\A
LOCATION
BRIO REPINEMV SITE ENCLOSURE
SAMPLE OSSION BY
ROSSXLAAKSO
DATE I\1«\B1
TIMK
SAMPLE
NUMBER
CNIOIS
CMIOIT
CNI01I
CNIOIS
CMI01B
cmosa
CHI01T
011041
CH104B
CN104B
DEPTH
0- . §
0" -
0" -
o* -
o- -
o- - B-
o- -
o- -
0* - B"
0« - B-
SAMPLE
PNTS
DEBT. /CONTAINER SIZE
CHEN MDIA OTHER
I
I
t
t
t
I
t
t
t
t
-
SAMPLE
METHOD
5
B
S
B
B
B
B
B
B
CHEST
NO.
SAMP
TYPE
B
B
B
B
B
B
S
B
PHtS-
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TIME
10
DATS
10
DATS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
ID
DAYB
1O
DAYS
10
DAYS
10
DAYS
TEST DESCRIPTION \ NOTES
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTSt SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS i SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS i SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTSt SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS i SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS i SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
BANTU CONTAINER DESCRIPTION
- I «! Jar (4 It)
. 1/1 Gal Jar (* It)
. | Pt *» Jar MSO-1)
- l/t Pt AB*J Jar (t30»l|
. VOA Vial
- Cera
- Whirl Pack
- I Qt Aak Jar
SAMPLE METHODi
Dlatvrba*
On4!at»rbad
Drill
Backhea
Trowal
Ballar
T
B Rand Cera
Split Spoon
10 Grab
II Caapoalta
It Rand Augar
SAMPLE TYPE l
S-Sall
M-Watar
O-OI1
X-Othar (daacrlba)
-------
2
z
>
r
»
M
W
O
90
H
SAMPLE LOCATION SUMMARY
PROJECT nun
nonet NUMBER 1014
CONDITIONS
Pug* 4 of
N\A
PIT/RORIMO) M.
N\A
LOCATION
MIO REPINERT ITTt ENCLOSURE
SAMPLE DBSION BT
ROSSVLAAKSO
DATE I\IB\B7
TIME
SAMPLE
NUMBER
CHIOS!
CM109T
CRIOBI
CH10BS
CM IOCS
CNIOT9
CNIOT7
OtPIR
0- .
0" -
o- - s"
o- -
o- .
o- -
0" -
SAMPLE DEST. /CONTAINER StZE
PUTS 6NEN M01O OTHER
f
I
f
f
I
I
t
SAMPLE
METHOD
S
S
CNEST
NO.
^
SAMP
TYrK
s
s
S
s
S
s
PRES-
ERVAT
CR1LL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TIME
10
DATS
10
DATS
IO
DATS
10
DATS
IO
DATS
to
OATS
IO
DATS
TEST DESCRIPTION \ NOTES
INORGANIC NUTRIENTS i SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS t SOLUBLE AMMONIUM.
NITRATE, PHOSPHOROUS
INOROANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS i SOLUBLE AMMONIUM,
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS l SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
COSTAINB* DESCRIPTIONi
» 1 Oal Aab Jut (4 It)
. I ! Jar (4 lt|
. |/S Oal J>r (t It|
. I Pt *» J«r (4*0*1)
I/I Pt A«b Jar (»Oal)
VOA.VlBl
Car*
Whirl Pack
. I Qt Amb Jar t«rb«d
S Drill
« Baekhe*
B Trowel
B Ballar
7 P«mo
B Hand Cora
Split Spoon
10 Drab
II Coiipoalta
IS Hand Augar
SAMPLE TTPEi
S-Soll
w-watar
0-011
X-Othar (daaerlba)
-------
SAMPLE LOCATION SUMMARY
2 PROJECT KAMI
MONSANTO
PROJECT NUMBER
z
>
r
»
m
«
O
»
H
S«IOI4
comiriora
Paga 5 of
N\A
HT/MMIM M.
N\A
LOCATION
BRIO REFINERY SITE ENCLOSURE
ROSSXLAAKSO
UTS
1\2«\ST
TIME
SAMPLE
NUMBER
CRIOIB
CNIOI*
CNI011
CHIOS?
CN1011
CNI03S
CHlOlt
CN104S
CRI041
CHIOftl
DEPTH
0" - "
o- -
o- -
o- . »
0" -
0" -
o- -
0- - »
0- - ft-
SAMPLE
PNYS
DEST./CONTAINI
CHEN MBIO
P
P
P
P
P
P
P
P
P
P
m SIZE
OTHER
SAMPLE
METHOD
S
ft
ft
S
ft
ft
ft
S
S
S
CREST
NO.
SAMP
TYPE
S
S
S
S
S
S
S
S
S
S
PRES-
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TIME
10
DAYS
10
DAYS
1O
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
TEST DESCRIPTION \ NOTES
VOLATILEXORCANICS
VOLATILEVOROANICS
VOLATILEVOROANICS
VOLATILE\OROANICS
VOLATILE\OROANICS
VOLATILEVOROANICS
VOLATILEVOROANICS
VOLAT I LB\OROAN I CS
VOLATI LEXOROANICS
VOLAT I LEXOROAN I CS
CONTAINER DESCRIPTIONi
I 0*1 AMI t*g (4 lt»
I tal Jar (4 It|
!/ Oal 3»r (3 It)
I Pt Aab Jar |4ftO*l)
I/I Pt A*b Jar (IftOall
VOA Vial
" Cora
Whirl Pack
1 qt Aab Jar (SO>)|
SAMPLE METHODi
Dlatarb«d
Drill
ackhoa
Trowal
Ballar
S Rand Cera
S Split Spoon
1O Drab
II CoBpoalta
IS Hand Auger
SAMPLE TYPEi
S-Soll
W-Matar
0-011
X-Othar (daacrlba)
-------
SAMPLE LOCATION SUMMARY
3
5
JO
n
«
O
so
H
PROJECT NAM MONSANTO
PROJECT NUMBIN M6IOI4
CONDITIONS
P»0« ft of
N\A
rtT/MMIIM Ml. N\A
LOCATION
BRIO REFINERY SITE ENCLOSURE
SAMPLE DESIGN BY
ROSS\LAARSO
DATE 1 M*\a7
TIME
SAMPLE
CR10BS
CHIOS*
CHIOS*
CHIOS?
CN1OTI
CH10TS
CM107S
BCPTM
O" -
-
0" -
0" - *
0" -
e- . -
o- - §
SAMTLI D«ST. /CONTAINER St»
PWrS CREM MSIO OTHER
r
r
r
r
r
r
»
SAMPLE
METHOD
a
s
s
CHEST
HO.
SAMP
TTW
s
1
t
s
t
s
PRES-
ERVAT
CHILL
CHILL
CHILL
CHlfc.b
1
CHILL
CHILL
CHILL
HOLD
TIME
10
DAYS
10
DAYS
10
DAYS
. 10
DAYS
1O
DAYS
10
DAYS
1O
DAYS
TEST DESCRIPTION \ MOTES
VOLATI LEVOROANICS
VOLATI LBVOROAMICS
VOLATILENOROAHICS
VOLATILEVOROAHICS
VOLATILEXOROANICS
YOLATILE\OROAHIC«
VOLATILB\OROAHIC«
VOLATI LEXOROANICB
DESCHIPTIOMl
I ! A*te Jmg (4 lt>
| tol Jar |4 It)
I/S 0«1 J»P |t It)
i rt Aak Jar («»»»)
l/» Pt A»b Jar (»»0»1J
«OA Vial
Cora
Mhlrl Pack
I Qt Aab Jar |»5O»1)
SAMPLE
Dlat«rb«d
On4lat«rb*4
Drill
Baekhoa
Trowal
a liar
T
1O
11
It
PUMP
Hand Cora
Split Spoon
Orab
Coapoalta
Hand Augar
SAMPLE TYPE.
Soil
0-011
X-Oth*r (d«acrlb*)
-------
SAMPLE LOCATION SUMMARY
PROJECT KAMI MONSANTA
PROJECT NUMBER IO14
COHDITIOM9
Pag«_
N\A
of
n
M
z
SB
M
O
90
H
PIT/BORING M. N\A
IOC AT I OH MIO 4EPINSRY SITE ENCLOSURE
SAMPLE DESIGN BY HOSSVLAAESO
DAT!
I\»«\B7
TIME
SAMPLE
NUMBER
CHIOI4
CNiois
CH1O11
cHioat
CNIOIO
CH10S4
CHI OSS
CMIO«t
CN104B
CH10BO
DEPTH
0" - "
o» -
0" - "
o- -
P" -
o« -
O" - "
o- -
o- - "
o- - "
SAMTU MST./COHMimil SIZE
nm CHEN mio OTHER
I
i
i
i
t
t
t
t
i
t
SAMPLE
METHOD
5
S
5
S
S
a
S
S
CHEST
NO.
SAMP
TVPB
S
S
S
S
S
S
S
P*ES-
EltVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
MOLD
TIME
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
1O
DAYS
1O
DAYS
10
DAYS
to
DAYS
10
DAYS
TEST DESCRIPTION \ NOTES
MICRO SIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
INSD OBSCHIPTION!
- 1 ! Aak JH« (4 It)
- I tal Jar |4 It)
- l/fl tal Jar (» It)
- i Pt Aab Jar <«»O«1|
- I/I Pt Aab Jar (SSOal)
WOA Vial
- - Cora
- Whirl Pack
- | qt AMD Jar («SOal)
LB MEIBODi
Dlattrbad
Ondlatvrbad
Drill
Baekhoa
Trowal
Bailor
t r««p
B Hand Cora
B Split Spoon
1O Grab
11 Compoalta
IS Hand Auflar
SAMPLE TYPBi
S-Soll
M-Matar
O-O11
X-Othar (d
r*v J/l/iT (SAMPLE.PORI
-------
M
5
n
O
90
H
WTW
PROJECT RANK
PIT/BORIN*.
SAMPLE DM!
SAMPLE LOCATION SUMMARY
p*a*_
of
MONSANTO
PROJECT RBMBER
1014
CONDITIONS
N\A
NO. N\A
LOCATION
MIO REFINERY SITE ENCLOSURE
am n ROSS\LAAMO
DAT! 1\1«\«7
TIME
SAMPLE
NUMBER
CHIOS*
CHIOS*
CH10«1
CM10««
CN10TO
CN1074
DEPTH
0" -
» - "
o- -
0" - «
0" - «"
0" -
SAMMJI DUT./CONTMHER SIZE
mTB CRBM MBIO OmER
I
I
I
I
I
f
SAMPLE
METHOD
S
s
S
s
9
S
CHEST
NO.
SAHP
TYPE,
S
S
MES-
EKVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TINE
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
to
DAYS
TEST DESCRIPTION \ NOTES
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
BANTU CONTAINER DESCRIPTIONt .
A - 1 ! A«b Jo« |« It)
- 1 tal J«r |4 It)
0 - I/I tel J«r ft It)
0 - 1 Pt tab Jar |4SO»1)
I - I/S Pt A»b Jmr (ISO*))
r - to* vui
- " Car*
- NMrl P*ek
I - 1 Qt A*h J«r ftSOal)
SAMPLE METHOD i
Brill
Backho*
Ballvr
1 P»«p
B R*nd Cor*
B Split Spoon
10 Or«b
11 CoBpoaita
It Rana Augar
S-Soll
M-Matar
O-OI1
X-Othar (daacriba)
(SAMPLE.PORI
-------
/
4
\
- /
3
2
/
1
C N 1 M
CH1099
CH1088
CH1097
CHIOtft
CH1093
CH1092
CH1091
CH1090
CH1097
CH1096
CH1095
CH1094
CH1101
CH1100
CH1099
CH1099
C N 1 M
29
22
15
8
1
POST
EXPLANATION
C CONTROL
N -NUTRIENT ADJUSTED
1 -INOCULATED
UOXEA; M
L REMOVE S RANDOM CAMPLES FROM EACH
QUADRANT AND COMPOSITE.
2. SAMPLES SHOULD SB REMOVED PROM THE
TREATMENT ZONE (TOP ')
9. SAMPLKS SHOULD BE REMOVED PROM THE
CENTRAL 10- OP EACH TREATMENT LANE
MULTIPLE INOCULATED
*«
4
»w^4^VVw
::«CMift
.--4
.-.««
....«««
-<«*
F r f) v /i
iQQQ^|^E^^B3BECjDQS39
SRIO SITS TASK FORCe
BRIO PROCESS AREA
SAMPLE PLAN
FEBRUARY 1, 1»}7
W*U ^M, ** ]B-« * . .
161014
REPORT
L-ll
-------
90
n
O
90
H
SAMPLE LOCATION SUMMARY
HONSANTO
PROJECT NUMBER
101*
CONDITIONS
Paaa I of t
NNA
PIT/BORIM NO.
N\A
LOCATION
MIO REP1NERT 9ITt ENCLOSURE
SAMPLI OESION IT
ROSS\LAAESO
DATS
«\1\S1
TIME
I3t00
SAMPLE
NUMBER
CNIOM
CHIOST
CNIOM
CNIOM
CRIOfO
CNIOSI
CNIOtt
CN1M1
CHI O*4
CNIOtt
birr*
o* -
o» - «
-
o- -
0" -
- -
0" -
o- -
o- -
o- -
SAMPLE
nrrs
MST./CONTAINM MZB
CNUI NVIO OTNKN^
I
t
I
I
f
f
I
t
I
I
9M«rtB
HBTHOD
. t
s
CHEST
NO.
>
AMP
TVrK
s
s
t
t
s
s
*
PHBS-
BRVAT
CNILt
CHIU.
CNIU
CNItt
CNIU.
emu.
CHILL
CHILL
CNILL
CNILL
HOLD
TINE
10
OATS
to
DATt
10
OATS
10
OATS
1O
OATS
10
OATS
1O
OATS
IO
OATS
IO
OATS
IO
OATS
TEST DESCRIPTION \ NOTES
INOMANIC NUTRIENTS t SOLUSLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS i SOLUSLE AMNONIOM.
RITRATB, PHOSPHOROUS
INOROANIC NUTRIENTS, SOLURLE AMMONIUM.
INORGANIC NUTRIENTS. SOLUSLE AMMONIUM,
RITRATB . PHOSPHOROUS
INOROANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS ,
INOROANIC NUTRIENTS! SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS i SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS t SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INONOANIC NUTRIENTS! SOLUBLE AMMONIUM.
NITRATE, PHOSPHOROUS
INOROANIC RUTRIEHTSt SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
SAMPLE CONTAINER DESCRIPTION,
I a«l Aab Jo« (4 It)
I Oal Jar |4 It)
1/1 9ml Jar |» It)
I Pt Aab Jar |4SOal)
I/a Pt A>b Jar (ISOal)
VOA Vial
f Cor*
Whirl Pack
I Ql Amb Jar
SAMPLE METHOD f
UndJaturbad
Drill
Backhea
Ballar
T Puap
Rand Cara
S Split Spoon
IO Drab
11 Coapoalta
11 Rand Auger
SAMPLE TTPBi
S-Sol)
M-Watar
O-OII
X-Othar (daacrlba)
-------
2
>
r-
M
n
O
JO
H
SAMPLE LOCATION SUMMARY
PROJECT HMIt
MONSANTO
PROJECT mimeit
1O14
CONDITIONS
Pag« I at 1
N\A
PIT/BORIM m.
N\A
LOCATION
me REPINERY tin ENCLOSURE
M»tS DUIBN IT ROSS\LA»MO
DATE
TIME
I3t00
AMTLK
CHIO«t
CRIOM
CHI 0*1
!
otrra
- -
-
-
.
MMTU OUT./COIRMmi «Itt
fwn CMSM mio ornn
s
I
I
t
SAMPU
HI moo
cnsr
M.
ANT
rrpK
NtlS-
EMVAT
CNttL
CNXLt
CHSLL
CMtLt
oto
TIME
10
OATS
10
DAY*
10
BAY*
10
OATS
TEXT DESCRIPTION \ NOTES
INOMANIC NimilENTS: SOLUBLE AMMONIUM.
NITRATE. PNOSniOROUS
INOROANIC NUTRIENTS i SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
IROROANIC NUTRIENTS! SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROARtC NUTRIENTS l SOLUBLE AMMONIUM.
NITRATE, PHOSPHOROUS
AMTU COBTAIHEH DESCRIPTION
. I Oal Aab Jufl (« lt|
I Om\ Jar <«
I/I Oal Jar
1 Pt * Jar
I/* H A*b Jar
VOA Vial
- Cora
NMrl Pack
I Qt »«b Jar (»50»l)
>*)
I* >«»
(4»0»l|
SAMPLE METHOD t
Dlatarbad
On'latarbad
Drill
ackhoa
Trowal
allar
10
II
IJ
Hand Cara
Split Spoon
Crab
Co«>poa 11 a
Hand Auger
SAMPLE TYPEi
S-Sell
w-Wal*r
0-011 '
X-Oth*r Idaacrleal
-------
. /
/
4
\
/
3
\
/
2
\
/
7
1
\
\
C N I M
CHO«45
CHOS4*
CHOa47
CHoa4a
CHOM1
CHO«42
CHOM3
CMOS44
CHoaa7
CHoaaa
CHoaaa
CH0840
CHoasa
CHoaa4
CHoaaa
CHoaaa
CNoaai
CHoaa2
CHoaaa
CHoa«4
CHoac?
CHoaaa
CHoaaa
CHoaao
CHoaaa
CHoaa4
CHoaaa
CHoaaa
CHO«4«
CHoaao
CHoaai
CNoaax
CHOI77
CHoara
CHoa7a
CHoaao
CNoara
CHOa74
CHoa7a
CHoa7a
CHoaaa
CHoaro
CHOa71
CHOS7t
CHoaaa
CHoaaa
CHoaar
CHoaaa
CHoaaa
CHoa»4
CHoaaa
CHoaaa
CHoaaa
CHoaao
CHoaai
CNoaaa
CHeaaa
CHoaaa
CHoaar
CHoaaa
CHoaai
/oaot
CNoaaa
/oaoi
CHoaaa
/oaoo
CHoaa4
C N 1 M
29
1
m
22
8
FIELD BLANKS
CHOa«7
CHoaaa
i
1
POST
EXPLANATION
C CONTROL
N -NUTRIENT ADJUSTED
1 - INOCULATED
NnTPa: M MULTIPLE INOCULATPD
1. REMOVE 9 RANDOM SAMPLES PROM EACH
QUADRANT AND COMPOSITE.
X. SAMPLES SHOULD SB REMOVED PROM THE
TREATMENT ZONE (TOP a*)
a. SAMPLE* SHOULD »E REMOVED PROM THE
CENTRAL W a* mte-.u TBBATUBU* i *u*
****^J**J*4?W 1
"rina
QnQ^pjpjpjpjRBjQjD^QlKQE
RIO SITE TASK PORCE
RIO PROCESS AREA
SAMPUNO AREA
PESRUARY ia. iaa7
^^f^mtmmmlimiU^mmlimimlimfiii^HftiHffi fMicTiMWl^^rvV-tr^yflr l>rHfH^WRI
IQI4/1-FINM REPORT L-14
-------
3
3
PI
O
JO
H
SAMPLE LOCATION SUMMARY
PROJECT NAME HONSANTO
PROJECT mjMBKR
1014
CONDITIONS
N\A
M.
N\A
LOCATION
BRIO MtriNERT »TI ENCLOSURE
AHPtl DESION MT MMVUUUtM
DATE
TSHK
tt«0 - 13:30
SAMPLE
MOUSE*.
CHOBM
010*37
CM0041
CSJ004S
CM004*
CROSS*
CHOOST
cirasoi
CMOS*
CROOO*
DEPTH
o- .
0" -
o- - «
o- -
-
o« .
" -
-
o- -
0- - "
MMTU »t»T. /CONTAINER «IZE
ms aim mio OTNER
i
X
t
i
t
l
i
t
t
t
lANPtl
NETNOD
S
S
1
»
S
cnrr
no.
SAMP
TTPE
8
a
s
f
*
*
*
PRKS-
CftVAT
CHttt
CHILL
CNItt
CNXU
CMIU
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
rim
10
DAT*
10
DATS
10
DAT*
10
DAT*
10
DAT*
10
DAT*
1O
DAT*
to
DAT*
10
DATS
10
DATS
TEST DESCRIPTION \ MOTES
TOTAL OROANIC CARSON.
SASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL ORGANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON,
BASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABtES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABtES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
SANK* CONTAINER DESCRIPTION i
t «! AM* Jo« (4 It)
1 0*1 Jar 14 It)
1/1 Oal J«r |1 It)
I Pt A«k J*r (450«1)
1/1 Pt A«b Jar (ISOall
VOA Vial
* Cor«
Mhlrl Peek
I - I Qt AMb J«r (soul)
SAMPLE METRODt
Dl«t«rb«d
Ondl*t«rb«d
Drill
Baekho*
Trowel
IUr
Hand Cor*
Split Spoon
1O Or»b
It COBpevlt*
It MM* Auger
SAMPLE TYPEl
S-Soll
M-u«t«r
0-011
X-Oth«r
-------
I
so
PI
"0
O
JO
H
SAMPLE LOCATION SUMMARY
PROJECT HAH!
MONSANTO
PROJECT NUMBER
1014
CONDITIONS
NNA
PIT/BOR1NO NO.
M\A
LOCATION
KIO REPINERT SITE CNCLOSOM
SAMPLE DCSION BT
ROSSXLAAKSO
DATE 1\1«\I7
TIHB
tiOO -
SAMPLE
NUMBER
CHOIT3
CNO«77
email
CHOOSS
CNOSSS
CNOM*
CNOMT
cmtoa
DEPTH
o- -
o- -
-
o- -
0- - "
-
-
" - "
SAHPtt
PUTS
DtST./COIITAIinil StZI
c«eM HBIO onen
t
I
X
t
I
t
t
t
SAHPU
NBTIOD
CHKST
MO.
SAMP
TTFB
s
s
s
nuts-
EMVAT
C»ILl
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TIME
10
DATS
to
DATS
SO
DATS
10
DATS
10
DATS
10
DATS
10
DATS
10
DATS
TEST DESCRIPTION \ NOTES
TOTAL OMOANIC CARSON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABtES
TOTAL ORGANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLES
.
SAMPLE CONTAINER DESCRIPTIONi
A - I 0*1 Aab Juff (4 It)
D - 1 9ml Jmr (4 It)
C - I/* 0*1 Jar |1 It)
D - I Pt Aab Jar (4SO>1|
E - I/I Pt Aak Jar |I90al|
P - VOA Vial
O - Cora
H - Whirl Pack
I - I Qt *«b Jar (*»0«1)
SAMPLE METHODI
Dl«tt§rba4
On41at«rba«
Drill
ckhoa
Trowal
Ballar
T
S Hand Cera
t Split Spoon
10 Orab
11 CoBpoalta
It Hand Auger
SAMPLE TYPEi
S-Soll
H-Matar
0-011
X-Othar (dcccrlb*)
-------
SAMPLE LOCATION SUMMARY
P»0*_
Of
PROJECT NAME
MONSANTO
PROJECT KUMBER
1014
CONDITIONS
N\A
PIT/BORINQ M.
NVA
LOCATION
RIO RCPINCRT SITE ENCLOSURE
O
90
H
SAMPLE DCSION IT
ROSSVLAAKSO
DATE
i\l«\B7
TIME
:00 - 13:30
SAMPLE
NUMBER
CMBS4
cwsaB
cms4a
CNOS4*
CHOSM
CMBB4
CNOBSB
CNOM1
CMS**
CHDD10
DEPTC
0- - «
0" -
.«- - «
-
0" . »"
- -
" -
-
0" -
0" - '
SANK! OEST. /CONTAINER SIZE
pinrs CHEN KSIO OTHER
t
t
I
s
I
I
t
t
t
i
SAMPLE
METHOD
S
S
CHEST
HO.
SAMP
TYPE
S
S
s
s
s
s
s
PRES-
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TIME
10
DATS
to
DATS
10
DATS
14
DAYS
10
DAYS
10
DAYS
to
DAYS
10
DAYS
10
DAYS
10
DAYS
TEST DESCRIPTION \ NOTES
INOROANIC NUTRIENTS t SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS! SOUfflLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORQANIC NUTRIENTS I SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS t SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS I SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROARIC NUTRIENTS! SOLUBLE AMMONIUM.
NITRATE. PNOSPNOROUS
INORGANIC NUTRIENTS I SOLUBLE AMMONIUM.
NITRATE. PNOSPNOROUS
IROROARIC NUTRIENTS! SOLUBLE AMMONIUM.
NITRATE. PNOSPNOROUS
INOROANIC NUTRIENTS t SOLUBLE AMMONIUM.
NITRATE. PNOSPNOROUS
INOROANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
SAMPLE CONTAINER DESCRIPTION.
A - 1 «! Aab Ju« (4 It)
D - I 0«1 J*r (4 It)
C - I/I 0*1 J*r « lt|
D - I Pt Aafe J«r (4»0«t|
E - l/> Pt Amb Jar (2>0al|
P - VOA Vial
0 - « Cor*
R - Whirl Pack
I - I Qt Anb Jar
SAMPLE METHODi
Disturbed
Ufi4l*t«rb*d
Drill
Backho*
Trowel
Ballar
T P«*p
Rand Cor*
Split Spoon
10 Grab
11 CoBpoalt*
12 Hand Augtr
SAMPLE TYPE:
S-Soll
H-Mat*r
0-011
X-Otber |d*»crlb«)
-------
SAMPLE LOCATION SUMMARY
J2 PROJECT NAME MONSANTO
PROJECT NUMBER BB1014
CONDITIONS
Pafa
N\A
or
PIT/MRINO NO.
N\A
LOCATION
RIO RtPINERY SITE ENCLOSURE
SAMPLE DBStON IT
ROSSXLAAKSO
DATE 1M«AB7
TIME
iOO - 13:JO
M
O
90
H
SAMPLE
NUMBER
CMOIT4
CNOSTB
CROBSt
CROBBS
CNOBSO
CROSB4
CHOBBS
CNOSOl
DEPTH
0" -
o- -
o- - "
o- -
o- - "
« . 0"
-
o> -
V
SAMPLE
PNTS
OEST./f
CNEM
t
t
t
1
I
1
S
I
rOHTAINI
M»IO
!R SIZE
OTHKR
SAMPLE
METHOD
t
CHEST
NO.
%^
SAMP
TTPE
S
S
S
S
a
PRIS-
ERVAT
CRILL
CRILL
etr-
Ch
CRILL
CHILL
CXIU.
CHILt
HOLD
TIME
10
BAYS
10
DATS
)
}
Uf.lt
10
OATS
10
DATS
10
DATS
10
DATS
TEST DESCRIPTION \ NOTES
INORGANIC NUTRIENTS I SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS t SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS i SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS I SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS t SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS 1 SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS i SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS t SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
SAMPLE CONTAINER DESCRIPTION!
A
B
E
P
0
R
I
1 Oal ABB J«*
1 Oal Jar
1/1 Oal Jar
1 Pt ABb Jar
1/1 pt ABb Jar
VOA Vial
Cora
Whirl Pack
1 qt ABb Jar
(4 It)
14 lt|
(t lt|
|4SOal|
(ISOBll
(SOBll
SAMPLE METHOD!
Dlatvrbad T
Undlat«rba4 B
Drill
aekhoa 10
Trowal 11
Ballar 11
Rand Cora
Split Spoon
Grab
CoMpoalta
Hand Auger
SAMPLE TTPEi
S-Soll
H-Mat*r
0-011
X-Othar (daaerlbal
tv 1/J/4T
|SAMPLE.PORI
-------
l^^^n
PROJECT mum
SAMPLE LOCATION SUMMARY
MONSANTO
PROJECT.NUMBER
Ml 014
CONDITIONS
Paga.
N\A
of
2 PIT/DORINQ HO. NXA
f» SAMPLE DESMN RY
LOCATION BRIO REFINERY SITE ENCLOSURE
ROSSXLAAKSO
DATE
»\J«\S7
TIME 9:00 - 13:30
«AHPU
emus
CNOSSt
CHOS4S
CNOS41
CROSS 1
CROSS*
CROSS*
CNOSSS
CMCCT
CMM71
MPTH
0- - "
0" -
o- . -
0" - "
0- - »
0" - "
0" -
0" -
o- - »
o* -
AMKI OKST./CONTAtmi StZI
nn CUM mio onu
r
r
r
r
r
r
r
r
r
r
3AHTU
HBTHOD
S
S
S
S
S
a
t
CRIST
NO.
SAMP
TYFE
S
S
S
S
S
S
S
S
S
S
nus-
MVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILI
CHILL
CHILL
HOLD
TIM!
10
DAYS
10
DAYS
10
DAYS
1O
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
TEST DESCRIPTION \ NOTES
VOLATILCXOKGANICS
VOLATILEXOKGMIICS
VOLATILEXORGANICS
VOLATILESORCANICS
VOLATILE\OftGANICS
VOLATILEXOROANICS
VOLATILEVOROANICS
VOLAT I LEXORCAN ICS
VOLATILEXOROAMICS
VOLATILEXOROANICS
O
- 1 tal AM J00 (4 lt|
- 1 ! J*r (4 lt|
- I/I Ml )mt |S It}
- 1 »t Aab Jtr (4SO>1)
- l/» Pt »^b 3*T (SSOHll
- VOA Vial
- Cor*
- Whirl Pack
- l Ot ABO Jar |«ftOal)
S-Soll
Drill
DMtklMM
TraiMl
S Rand Cora
* Split Spoon
10 Orab
II CoBpoalta
IS Rand Augar
0-011
X-Oth«r (d««crlb«)
r«v 1/3/S1- (SAMPLE.POR)
-------
SAMPLE LOCATION SUMMARY
P«ge_
PROJECT NAME MONSANTO
PROJECT NUMBER ««IO14
CONDtTtONS
N\A
z
r*
90
M
«
o
»
H
PIT/EORINO NO. N\A
LOCATION
RIO REPINERY SITE ENCLOSURE
SAMPLE DESIM HI
»OM\LAAHSO
BATS *U«\»7
TIM!
lOO -
AMPLE
CNOSTS
CNOSTS
CROM3
CNOM?
CHOMt
CROSS*
«»
CNOMO
Mpn
« - «
0" -
0" - «"
0> _ (>
" - -
0" - «
o- -
0" - "
tAWU WtST./CONTAIIIIR II XI
PMYS CUM MIO OTMR
r
r
9
t
r
r
t
r
AMPU
NKTIIOD
B
S
S
S
CM*T
NO.
AMP
TY-PB
PMS-
IRVAT
emu
CHILL
CHILL
CMItt
CHILL
OltLL
CHILL
CHILL
OLD
TIN*
10
DAY*
10
OATS
to
DAY*
10
DAYS
10
DAYS
10
DAYS
10
DAYS
1O
DAY*
TEST DKSCRIPTION \ NOTBS
VOLATILCVOROANICS
VOLATILINOROANICS
VOLATILCVOROANICS
VOLATZLIXOROANIC*
VOLATILIVOROANIC*
VOLATIUVOROANICS
VOLATIUXOROANICS
VOLATILHNOROANICS
MMPU OOWTAINM DSSCMlPTIONi
A - 1 «! Aab Jn« (4 lt»
- 1 0*1 J«r |« It)
0 - I/I *! Jar |I Itl
D - 1 Pt Aab J«r |«Stal)
- I/I Pt Mb Jmr (I80«l>
r - to* vi«i
- 4" Cora
- Whirl Pack
I - 1 Qt AMb J«r (»80»1)
SAMPLE
Drill
tackho*
Trowel
taller
R«n4 Cor*
Split Spoon
1O Orsb
11 Coapoait*
II Hand Au0«r
SAHTU TYPE i
-oil
N-M«t«r
O-O11
X-Othor Idescribe)
rev J/J/17
(SAMPLE.POR)
-------
SAMPLE LOCATION SUMMARY
Of
PROJECT MM! MONSANTO
PROJECT NUMBER 161014
CONDITIONS
N\A
PIT/BORING NO. N\A
LOCATION
BRIO REFINERY SITE ENCLOSURE
SAMPLE OISIOII BT
ROSSVLAAKSO
DATI
I\»6\»1
TINE »:00 - 13:3O
SAMPLE
NUMBER
CHO.3.
CHOC40
CHOB44
CHO*4*
CBOBSS
CM*S*
CM****
CBJO**4
CM****
CM*7t
DEPTH
0" -
o- -
o- -
o- -
* - -
""
-"
.....
.....
.....
AMPLE
DEST. /CONTAINER SIZE
CHEM MBIO OTHER
D
D
D
D
D
D
D
'
0
*
SAMPLE
METHOD
S
S
S
CHEST
NO.
SAMP
TYPE
S
S
S
S
'
*
*
PRES-
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TIME
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
BAYS
10
DAYS
10
DAYS
10
DAYS
TEST DESCRIPTION \ NOTES
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
90
M
V
O
90
H
K>
- 1 *! Aafc J»g (4 Itl
- I Ml Jar 14 it)
- I/a *! Jar (I It)
- 1 Pt Aat> Jar (4SO>1|
- l/S Pt Art Jar
- VGA Vial
- Cera
-Whirl Pack
- 1 Qt Art Jar
Otaturbad
Ondlatarbad
Drill
Baekhea
Trowal
Ballar
B Hand Cera
t Split Spoon
10 Orab
11 Coapeelta
12 Hand Auger
S-Soll
N-Natar
0-011
X-Oth*r (daaerlba)
raw 2/3/BT I SAMPLE.POR|
-------
SAMPLE LOCATION SUMMARY
of
O V A
PROJECT NAME
HONSANTO
PROJECT NUMBER
1014
COMOITIONS
N\A
PIT/BORING MO. N\A
LOCATION
RIO REPINCRT SITE ENCLOSURE
SAMPLE DCSION BY
NOSSMAAKSO
DATE I\1«\I7
TIME »:00 - 13:30
so
W
O
SO
H
SAMPLE
NUMBER
CHO«T«
CNOBBO
CHOBB4
CNOB»t
CHOOM
CNOBM
. DEPTH
0- . B"
0" - B"
0" - "
0- - "
0- - B-
0- - B-
SAMPLE DEST. /CONTAINER SIZE
PNYS CHEN NBIO OTHER
.
0
0
D
P
SAMPLE
METHOD
S
9
S
B
B
B
CHEST
HO.
SAMP
TTPE
S
*
S
S
PRES-
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TIME
10
OATS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
MYS
TEST DESCRIPTION \ MOTES
NICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
NICRO BIOLOGICAL EVALUATION
NICRO BIOLOGICAL EVALUATION
HICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
K>
N»
BANTU CONTAIN!* DCSCHlrTIONi
A - 1 *! Aa» * t« lt|
»-!! J«r |4 Itl
C 1/t 0*1 Jmr |t lt|
D - I Pt Art Jar |4BOalt
- i/t Pt Art Jar |1SO«1|
r - VOA Vial
0 - ' Cor*
- Whirl Pack
I - 1 Qt Art Jar |«SOal|
AMPLE Nil HUD i
Dlatwbad
Drill
Baekhoa
Trowel
allar
T
B Ran4 Cora
B Split Spoon
1O Orak
11 Caapoalt*
11 Rand Auger
SAMPLE TYPEI
S-Soll
N-Hatar
0-011
X-Othar (daaerlba)
rav l/3/«T
(SAMPLE.FOR I
-------
/
f
4
\
3
. \
r
2
\
/
/
' 1
C N I M
CHO922
CH0921
CH1134
CH0920
CH0919
CH1133
CHO918
CH0917
CH1132
CHO916
CHO91S
CH1131
CHO930
CHO929
CH1138
CHO92S
CHO927
CH1137
CHO926
CHO92S
CH1136
CHO924
CHO923
CH1135
CHO938
CHO937
CH1142
CHO936
CHO93S
CH1141
CHQP34
CHO933
CH1140
CHO932
CHO931
CH1139
CHO946
CHO949
CH114J
CHO944
CH0943
CH114S
CHO941
CHO941
CH1144
CH0940
CHO949
CHO939
CHO947
CH1U3
C N 1 M
29
m
22
15
8
FIELD BLANKS
CHO949
CHO9SO
1
POST
CX PL A NATION
C -CONTROL
N -NUTNIENT ADJUSTED
I INOCULATED
M MULTIPLE INOCULATED
1. REMOVE 3 RANDOM SAMPLES PROM THE 3 CENTER
SECTIONS IN EACH QUADRANT AND COMPOSITE
S. SAMPLES SHOULD SE REMOVED PROM THE
TREATMENT ZONE (TOP '»
3. SAMPLES SHOULD SE REMOVED FROM THE
CENTRAL 10 OF EACH TREATMENT LANE
I61014/1.FIVAL REPORT
SRIO PROCESS AREA
SAMPLING PLAN
MARCH 2S.19J?
-9*3
L-23
-------
n
M
>
r*
O
90
H
SAMPLE LOCATION SUMMARY
Paga,
of
PROJECT NAME
BRIO TASK FORCEX
MONSANTO
PROJECT NUMBER 861014
CONDITIONS
NXA
PIT/BORING NO.
NXA
LOCATION
BRIO REPINERY SITE ENCLOSURE
SAMPLE DESION BY RO3SXLAAKSO
DATE
3X25X87
TIME
9:00 - 12:30
SAMPLE
NUMBER
CH0915
CH0917
CH0919
CH0921
CHO923
CH0925
CH092T
CN0929
CH0931
CH0933
DEPTH
o- -
0" - «"
0" - 6"
0" - 6"
0" - "
0" - "
0" - 6"
0" - 6"
0" - 6"
0" - 6"
SAMPLE DEST. /CONTAINER SIZE
PNYS CHEN MBIO OTHER
I
I
I
I
I
I
I
I
I
I
SAMPLE
METHOD
S
s
5
5
6 *
S
3
9
3
9
CHEST
NO.
SAMP
TYPE
S
S
S
s
s
s
s
s
s
s
P*ESr
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CNILL
CHILL
HOLD
TIME
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
TEST DESCRIPTION \ NOTES
TOTAL ORGANIC CARBON,
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
TOTAL ORGANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
TOTAL ORGANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
TOTAL ORGANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
TOTAL ORGANIC CARBON,
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
TOTAL ORGANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
TOTAL ORGANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
TOTAL ORGANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
TOTAL ORGANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
TOTAL ORGANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
SAMPLE CONTAINER DESCRIPTION:
A - 1 Gal A»b Jug (4 It)
B - 1 Gal Jar (4 It)
C - 1/2 Gal Jar (2 It)
D - 1 Pt A*b Jar (4SO»1)
SAMPLE METHODS
1 Disturbed
2 Undisturbed
3 Drill
4 Backhoa
i Trowel
7 PUMP
8 Hand Core
9 Split Spoon
10 Grab
11 Coapo«lt«
SAMPLE TYPE:
S-Soll
M-Hater
0-011
X-Other (describe)
-------
II
z
M
O
90
H
SAMPLE LOCATION SUMMARY
PROJECT NAME
BRIO TASK
HONSANTO
PROJECT HUMBER
61014
CONDITIONS
pag«_
NXA
FXT/BORIM M>.
NX*
LOCATION
BRIO REFINERY SITE ENCLOSURE
SAMPLE DESIGN BY
R03SXLAAKSO
DATE axasxsT
TINE
9:00 - 12:30
SAMPLE
NUMBER
CHOS3S
CH0937
CH0939
CH0941
CH0943
CN094S
CH0947
CH0949
DEPTH .
o- - "
0" - "
0" - 6"
0" - "
0" - "
0" -
0" - "
0" - "
SAMPLE DEST. /CONTAINER SIZE
PNYS CEEM MBIO OTHER
I
I
I
I
I
I
I
I
SAMPLE
METHOD
S
5
S
5
S
S
S
S
CHEST
MO.
SAMP
TYPE
S
S
S
S
S
S
S
S
PRES-
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TIME
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
TEST DESCRIPTION X NOTES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
TOTAL OROANIC CARBON,
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
TOTAL ORGANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
TOTAL OROANIC CARBON. DUPLICATE SAMPLE
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
TOTAL ORGANIC CARBON. FIELD BLANK
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
SAMPLE CONTAINER DESCRIPTION:
A - I Gal A»b Jug (4 It)
B - 1 Gal Jar (4 It)
C - 1/2 Gal Jar (2 It)
D - 1 Pt A»b Jar (4SOal)
E - 1/2 Pt A«b Jar (250*1)
m _ ttn* if I 1
SAMPLE METHOD:
1 Disturbed
2 Undisturbed
3 Drill
4 Backho*
S TroM«l
Ballar
7 Punp
Hand Core
Split Spoon
1O Grab
11 Co«po«lte
12 Hand Auger
SAMPLE TYPE:
S-Soll
M-Hater
0-011
X-Other (describe)
-------
^
j
e
H
M
«
s|
o
M
O
M
g
M O
««
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hi I
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-2
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8
«s
So
S
II
08
"3
8
0S
»
ii
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M
ex
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o
I
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X OO
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nx o M
o b
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ha w 9
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Ci
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I I I I I
W
161014/l-FINAL REPORT
L-26
-------
I
n
PI
W
o
so
H
SAMPLE LOCATION SUMMARY
BRIO TASK rORCIN
PROJECT HANI MONSANTO PROJECT NUMBER B61014
CONDITIONS
Page 4 - of 6
N\A
PIT/BORINO NO. N\A
LOCATION
BRIO REPINCRY SITE ENCLOSURE
SAMPLE DESION BY
MOSSXLAAKSO
DATE 3\25\87
TIME
9:00 - 12:30
SAMPLE
NUMBER
CH093*
CH093S
CH094O
CHO942
CH0944
CR094C
CHOt4B
CH0950
1
DEPTH
0" - "
0" - 6"
0" - ft"
0" - "
0" - a"
o- -
0" - '
0" - "
SAMPLE DEST. /CONTAINER SIZE
PNYS CHEM MBIO OTHER
I
I
I
I
I
I
I
I
SAMPLE
METHOD
S
s
3
S
S
s
s
s
CHEST
NO.
SAMP
TYPE
S
S
s
s
s
s
s
s
PRES-
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TIME
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
TEST DESCRIPTION V NOTES
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE, PHOSPHOROUS
INORGANIC NUTRIENTS t SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS DUPLICATE SAMPLE
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS FIELD BLANK
SAMPLE CONTAINER DESCRIPTIONt
A - 1 0*1 Art* Jug (« It)
- 1 0*1 Jar (4 It)
C - 1/2 0*1 Jar (2 It)
D - 1 Pt A»b Jar (4SOal)
E - 1/2 Pt Aab Jar (230al)
P - VOA Vial
SAMPLE METHODi
1 Diaturbad 7 Puap
2 Undlaturbad Hand Cora
3 Drill Split Spoon
4 Backhoa 10 Grab
S Trowal , " Coapoalta
ft Ballar » Hand Augar
SAMPLE TYPE:
S-Soll
M-Hatar
0-011
X-Othar (daacrlba)
-------
so
M
O
90
H
SAMPLE LOCATION SUMMARY
BRIO TASK FORCES
PROJECT NAME MONSANTO PROJECT NUHBER M1014
CONDITIONS
Page S of 6_
N\A
PIT/BOMINO NO. NSA
LOCATION BRIO REFINERY SITE ENCLOSURE
SAMPLE DESIGN BY ROSSXLAAKSO
DATE
3\29\87
TIME 9:00 - 12:30
SAMPLE
NUMBER
CH1131
CHI 132
CHI 133
CHI 134
CHI 135
CH113*
CHI 137
CH113*
CHI 13*
t
Oil 40
DEPTH
0" - 6"
0" - 6"
0" - «"
0" - 6"
0- - "
0" -
0" - "
0" - 6"
0" -
0" - "
SAMPLE DEST. /CONTAINER SIZE
PHYS CREM MBIO OTHER
D
D
D
D
D
D
0
D
D
D
SAMPLE
METHOD
9
5
3
5
S
8
6
S
ft
ft
CHEST
NO.
SAMP
TYPE
S
S
S
S
S
S
B
B
B
B
PRES-
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TIME
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
to
DAYS
10
DAYS
10
DAYS
10
DAYS
TEST DESCRIPTION V NOTES
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOOICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOOICAL EVALUATION
MICRO BIOLOOICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOOICAL EVALUATION
MICRO BIOLOOICAL EVALUATION
r
N»
SAMPLE CONTAINER DESCRIPTION t
A - 1 Gal A»b Jug (4 It)
B - 1 Gal Jar
C - 1/2 Gal Jar
D - I Pt Aab Jar
(4 It)
(2 It)
( 430*1)
1290*1)
SAMPLE METHODt
1 Disturbed
2 Undivturbad
3 Drill
4 Backho*
TroNal
7
B
10
11
12
Pump
Hand Cora
Split Spoon
Grab
Coapoalte
Hand Auger
SAMPLE TYPE:
S-Soll
H-Hatar
0-01 a
X-Othar (daacrlbe)
-------
I
n
I-
90
w
«
O
»
H
SAMPLE LOCATION SUMMARY
C O V A
BRIO TASK rORCEV
PROJECT NAME MONSANTO
PROJECT NUMBER
61014
CONDITIONS
Pag* 6 of 6
N\A
PIT/BORING NO. N\A
LOCATION
BRIO REPINERY SITE ENCLOSURE
SAMPLE DESIGN BY
ROSSVLAAKSO
DATE 3\25\87
TIME 9:00 - 12:30
SAMPLE
NUMBER
CH1141
CH1142
CH1143
CH1144
CH1145
CH114*
1
DEPTH
0" - "
0" - 6"
0" -
0" - "
0" -
0" - "
SAMPLE DEST. /CONTAINER SIZE
PHYS CNEM MBIO OTHER
D
D
D
D
D
D
SAMPLE
METHOD
9
5
S
s
S
s
CHEST
NO.
SAMP
TYPE
S
S
s
s
s
s
PRE3-
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TIME
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
TEST DESCRIPTION. \ NOTES
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATIOH
MICRO BIOLOGICAL EVALUATION
SAMPLE CONTAINER DESCRIPTION:
A - 1 0*1 A«b Jug (4 It)
B - 1 0*1 Jar (4 It)
C - 1/2 Gal Jar (2 It)
D - 1 Pt A»b Jar <450«1)
E - 1/2 Pt Aab Jar (250*1)
SAMPLE METHODi
1 Dlaturbad
2 Undlaturbcd
3 Drill
4 Backho*
S TroM«l
7 ' Pu»p
Hand Cora
9 Split Spoon
1O Grab
11 Coapoalta
12 Hand Auaer
SAMPLE TYPEs
S-Soll
M-Hat«r
0-011
X-Othar (deacrlba)
-------
/
f
4
\
3
\
/
2
\
/
/
1
C N I
CH0342
CH0343
CH0344
CH0339
CH0340
CH0341
CH0336
CH0337
CH0338
CH0333
CH0334
CH0335
CH03S4
CH039S
CH03SB
CH0351
CH0352
CH0393
CH0348
CH0349
CH03SO
CH0345
CH0346
CH0347
C N
NOTES:
1. REMOVE 3 RANDOM SAMPLES FROM EACH
QUADRANT AND COMPOSITE.
2. SAMPLES SHOULD BE REMOVED PROM THE
TREATMENT ZONE (TOP 6')
3. SAMPLES SHOULD BE REMOVED FROM THE
CENTRAL 10' OF EACH TREATMENT LANE.
CM036C
CH036?
CH0368
CH0363
CH0384
CH0385
CH0360
CH03n
CH03«2
CH03S7
CH035«
CH039*
M
CH037»
CH037B
CH03tO
CH037S
CH0376
CH0377
CH0372
CH0373
CH0374
CH0369/
CH03I1
CH0370/
CH03B2
CH0371
1 M
::: :::«: z
::: ::*** H
29
(K)
22
15
8
FIELD BLANKS
CH03M
CH03«3
1
POST
iiffl iHpifasr~>TlflfcAl«a
RIO SITE TASK FORCE
::: ::**** BRIO PHOCESB AREA
"I ?23*i SAMPUMO AREA
I!. «,« APR.L 30. 1»S7
E
UA£
K-BBKWK?,7u?A=flM_
161014/l-FINAL REPORT
LOO
-------
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S61014/1-FINAL REPORT
L-31
-------
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861014/1-FINAL REPORT
L-32
-------
SAMPLE LOCATION SUMMARY
Pag*.
of __
I-
99
w
«
O
90
H
PROJECT NAMC
MIO TASK PORCK\
PROJECT NUMBER M1014
CONDITIONS
N\A
PIT/BOB.INO HO.
LOCATION
BRIO REPINERT BITE ENCLOSURE
BANTU DCSIBM BT ROSSVLAAESO
DATE 4\30\S1
TIME 0»iOO - 11:30
SAMPLE
CH0334
CH033T
CH034O
CRO343
CH034*
CH034S
CH03S9
CSJOBSB
CH03»S
CflOBBI
DEPTH
0- -
0" - "
0" -
0- - »
0" - S"
0" . »
o- -
0" - B"
o* -
o- -
SAMPLE
PHTS
BIST. /CONTAINER SIZE
CHEN MBIO OTHER
I
I
I
I
I
I
I
I
I
I
SAMPLE
METHOD
6
$
S
'
$
*
'
'
S
CHEST
HO.
SAMP
TTPB
B
B
B
B
B
B
B
B
B
S
PRES-
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HQLff
TIME
10
DATS
10
DATS
10
DATS
10
DATS
10
DATS
10
DATS
10
DATS
10
DATS
10
DATS
10
DATS
TEST DESCRIPTION \ NOTES
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
SAMPLE CONTAINER DESCRIPTION:
A - 1 Gal Aab Jug <« It)
B - 1 Gal Jar 14 It)
C - 1/2 Gal Jar <» It)
D - 1 Pt ABb Jar (4SOaJ)
R - I/a Pt Aab Jar (230«1)
P - VOA Vial
O - " Cor*
H - Whirl Pack
I - 1 Qt Aab Jar (»50»1)
SAMPLE METHODI
1 Dlaturbcd
2 Undlaturbvd
3 Drill
4 Backhe*
S Trowel
Ballar
T PIMP
Hand Cor*
Split Spoon
10 Grab
11 COMBO*It*
12 Hand Auger
SAMPLE TTPE:
S-Soll
H-Mat*r
O-O11
X-Oth*r (d**crlb*)
-------
SAMPLE LOCATION SUMMARY
SB
W
O
90
H
PROJECT HANI
riT/WMtlNO MO.
MIO TASK rORCEN
NOMSMTO
PROJECT NUMBER 1014
CONDITIONS
Pao« 4 of __
N\A
N\A
LOCATION
MIO REPINERT SITE ENCLOSURE
SAMPLE DESIO1I BT
ROSSXLAAKSO
DATE 4\30\»7
TINS
Ot:00 - 11:90
SAMPtS
CN03C4
CH0947
CHOS70
CM0979
,CH037S
CMO97S
CNO»S2
CH09S4
DEPIH
0" - "
e- -
0" - "
0" - "
o- -
0" - "
0" -
o- -
AMPU MST./eOKTAMER SIZE
PETS CMEM WZO OTIER
I
I
t
t
X
I
Z
z
SAMPLE
HETIOO
S
O.
TTPE
S
S
PRSS-
EKVAT
CHILL
CHILI
CEILL
MILL
cnu
taut*
CIZU
CtltL
OLD
TIME
10
DATS
to
DATS
to
DATS
10
DATS
10
DATS
10
DATS
10
DATS
10
OATS
TEST DESCRIPTION \ MOTES
ZMOROANie NUTRIENTS t SOLUBLE AHHONIUM.
NITRATE. PHOSPHOROUS
IMOROANIC NOTRIENTSt SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
IMOROANIC NUTRIENTS I SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
IMOROANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS t SOLUBLE AMMONIUM,
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS i SOLUBLE AMMONIUM.
MZTRATE, PHOSPHOROUS
ZNOROANIC NUTRIENTS i SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS DUPLICATE SAMPLE
INOROANIC NUTRIENTS s SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS PIELO BLANK
SAMPLE CONTAINER DESCRIPTION:
A - I 0*1 AMb Jug |4 it)
B - 1 0*1 J«r (4 It)
C - 1/2 Gal Jar (2 lt|
0 - I Pt A«b Jar («50«ll
E - 1/2 Pt Aab Jar (ISO»1)
P - VOA VU1
O - " Cor*
H - Whirl Pack
SAMPLE METHODi
Dlaturbad
Undlaturbad
Drill
Sackho*
Trowal
Bailer
7 Puap
S Hand Cor*
Split Spoon
1O Grab
11 CoMpoalt*
12 Hand Augar
SAMPLE TTPE:
S-Soll
H-Hat*r
0-011
X-Oth«r (describe)
-------
SAMPLE LOCATION SUMMARY
PROJECT KAMI
PIT/RORINO NO.
MIO TASK FORCES
MONSANTO
PROJECT KOMBUI
1014
CONDITIONS
Paga 5 of
H\A
UNA
LOCATION
RIO REFINERY SITE ENCLOSURE
O
90
H
SAMTU DESIGN M
ROSSVLAAKSO
DAT!
4\30\»7
TIM! 09:00 - 11:30
SAMTU
CROSS*
CHO33S
CN0341
CRO344
CNOS4T
CBO3S4)
osjMii
CMO3S*
CSM3SS
CH03«2
ovn
o- -
0" -
o- -
o- -
o- -
o* -
O" - "
0" -
o- -
0" -
MMPtl MrST./CONTAINUt 9IZI
m» CON MBIO OTNEN
0
D
0
O
D
0
0
0
D
D
SAMTLB
HtTNOO
S
OUST
NO.
SAMF
TVI-E
S
S
S
S
ntss-
EJtVAT
CHILL
CULL
CNILL
CHILL
CHILL
CHILL
onu
CHILL
CHILL
CHILL
OLD
TIMS
10
OAT*
10
DATS
10
BATS
10
DATS
10
DATS
10
DATS
10
DATS
10
DATS
10
DATS
10
DATS
TEST DESCRIPTION \ NOTES
MICRO IIOMOICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO SIOLOOICAL EVALUATION
MICRO RIOLOOICAL EVALUATION
MICRO IOMOICAL EVALUATION
MICRO IOLOOICAL EVALUATION
MICRO SIOLOOICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
SANK! CONTAINER DESCRIPTION t
A - I 0*1 Aab Jug (4 It)
- 1 O«l Jar 14 It)
C 1/2 0«1 Jar (1 It)
D 1 Pt Art Jar (4SO.I)
R - 1/2 Pt Aab Jar («<>))
V - VOA Vial
O - «" Cor*
N - Whirl Pack
i . i n» k_h Jar I9SOB1)
SAMPLE KETNODi
1 Dlaturbad
2 Undleturbad
3 Drill
4 Backho*
9 Trowal
Bailer
T Pu«p
Hand Core
Split Spoon
10 Grab
11 Coapoalt*
12 Hand Auger
SAMPLE TYPEi
S-Soll
W-Wat«r
0-011
X-Oth*r (describe)
-------
SAMPLE LOCATION SUMMARY
PKOJECT MAM
BRIO TASE FORCBN
PROJECT NUMMN
1014
CONDITIONS
Paga_« Of _
M\A
PIT/SORIM HO. N\A
LOCATION
BRIO REPINERY «ITI ENCLOSURE
O
SAMPU DESIGN B»
ROSSNLAAXSO
DAT* 4\30\«7
TIMS 0»iOO - 11:30
SAMPLE
NUMBER
CH03«5
CH03M
CM0371
CM03T4
I
CH03TT
CH03BO
DEPTH
o- -
0" - "
0" - "
o- -
0" - "
o- -
SAMPLE DE9T. /CONTAINER SIZE
ran an M»IO OTHER
D
D
D
0
0
0
SAMPLE
Nsnoo
s
s
s
9
'
CREST
NO.
SAMT
TVPE
S
S
s
s
s
PRES-
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TIME
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
TEST DESCRIPTION V NOTES
MICRO SIOLOOICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATIOH
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
SAMPU CONTAINER DESCRIPTION!
A - 1 Gal A«b JOB (4 It}
- 1 0*1 J«r (4 It}
C - I/a 0*1 Jar (t lt|
D - 1 Pt A»b Jar (460.11
E I/a Pt A»b Jar (ISOall
r - VOA vial
O - " Cor*
H - Whirl Pack
I - 1 Qt Aab Jar («50«1)
AMPLE METHODi
Dlaturbad
Umtlaturbcd
Drill
ackhoa
Trowel
Ballar
7 Pu*p
Rand Cora
Split Spoon
10 Grab
11 Coapoalta
12 Hand Aupar
SAMPLE TYPEi
S-Soll
N-Hatar
0-01J
X-Othar (daacrlba)
-------
I
*!
f"
90
M
O
»
H
SAMPLE LOCATION SUMMARY
Paga_
PROJECT NAME
BRIO TASK rORCEV
MONSANTO
PROJECT HUMBER 61014
CONDITIONS
N\A
PIT/BORINO MO.
M\A
LOCATION BRIO REFINERY SITE ENCLOSURE
SAMPLE DESIGN BY ROSSVLAAKSO
DATE 3\2ft\ft7
TIME
9:00 - 12:30
SAMPLE
NUMBER
CH091C
CH091B
CH0920
CH0922
CH0924
CH092*
CHOB28
CH0930
CHO*32
CH0934
DEPTH
0" - ft"
0- - ft"
0" -
0" - ft"
O" - "
0" - «"
o- _ i-
0" - ft"
0" - ft"
0" - ft"
SAMPLE
PHTB
OUT. /CONTAINER SIZE
CHEN MBZO OTHER
I
I
I
I
I
I
I
X
Z
I
SAMPLE
METHOD
ft
9
A
ft
ft
ft
a
ft
s
9
CHEST
NO.
SAMP
TYPE
S
S
S
s
s
s
s
s
s
PRES-
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TIME
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
TEST DESCRIPTION \ NOTES
INOROANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE, PHOSPHOROUS
INOROANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE, PHOSPHOROUS
INOROANIC NUTRIENTS: SOLUBLE AMMONIUM,
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS t SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE, PHOSPHOROUS
INOROANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
SAMPLE CONTAINER DESCRIPTION:
A - 1 Oal A«b Jug (4 It)
B - l Oal Jar (4 It)
C - 1/2 Oml Jar (2 It)
D - 1 Pt A»b Jar <430al)
t - 1/2 Pt A«b Jar <250«1)
P - VOA Vial
G - 6" Cora
SAMPLE METHODi
1 Disturbed
2 Undlaturbad
s Drill
4 Backhoa
S TroMal
6 Ballar
7 Pu*p
Hand Cora
Split Spoon
10 Orab
11 Conpoalta
12 Hand Augar
SAMPLE TYPE:
S-Soll
H-Hatar
0-011
X-Othar (deacrlbe)
-------
I
fl
r-
so
o
90
H
SAMPLE LOCATION SUMMARY
PROJECT MAMS
BRIO TASK FORMA
MONSANTO
PROJECT NUMBER
M1O14
CONDITIONS
Pags.
N\A
Of 6
PIT/BORINO NO. N\A
LOCATION
BRIO REFINERY SITE ENCLOSURE
SAMPLE DESIGN BY . ROSS\LAAKSO
DAT! 3\2S\«7
TIMS
t:OO - 12:30
SAMPLE
NUMBER
CNOS38
CH0937
CH0939
CH0941
CH0943
CH0949
CH0947
CHO»4f
DCPTN
0" - "
0" - "
0" - e"
O" - «"
0" - *"
0" - "
0" - 6"
0" - 6"
SAMPLE DEST. /CONTAINER SIZE
PNYS CNBM MBIO OTHER
I
I
I
I
I
I
I
I
SAMPLE
METHOD
9
8
8
8
8
8
8
9
CHEST
NO.
SAMP
TYPE
S
s
S
s
s
s
s
s
PRES-
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TIMS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
TEST DESCRIPTION \ NOTES
TOTAL OROANIC CARBON,
BASE NEUTRAL ACID EXTRACTABLES\VOLATILES
TOTAL OROANIC CARBON,
BASE NEUTRAL ACID EXTRACTABLES\VOLATILES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLESWOLATILES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLESWOLATILES
TOTAL ORGANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLESWOLATILES
TOTAL ORGANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLESWOLATILES
TOTAL OROANIC CARBON. DUPLICATE SAMPLE
BASE NEUTRAL ACID EXTRACTABLESWOLATILES
TOTAL ORGANIC CARBON. PIELD BLANK
BASE NEUTRAL ACID EXTRACTABLESWOLATILES
K»
In
SAMPLE CONTAINER DESCRIPTION:
A - 1 Gal A«b Jug (4 It)
B - 1 Gal Jar (4 It)
C - 1/2 Gal Jar (2 It)
D - 1 Pt A»b Jar <4SO«1)
SAMPLE METHOD:
1 Disturbed
2 Undisturbed
3 Drill
4 Backho*
7 Puap
Hand Core
Split Spoon
10 Grab
11 Composite
«> Hand Auoer
SAMPLE TYPE:
S-Soll
H-Wat«r
0-011
X-Other (describe)
-------
f-
98
O
90
H
SAMPLE LOCATION SUMMARY
PROJECT HAMS
BRIO TASK FORCES
MONSANTO
PROJECT NUMBER S61014
CONDITIONS
Page 5 of «
N\A
PIT/BORING NO. N\A
LOCATION
BRIO REFINERY SITE ENCLOSURE
SAMPLE DESIGN BY
ROSSXLAAESO
DATE
3\2S\87
TIME 9:00 - 12:30
SAMPLE
NUMBER
CH1131
CH1132
CH1193
CHI 134
CH1136
CH113*
CHI 137
CH113S
CHI 19*
CVll 40
DEPTH
0" - «"
0" - «"
0" - "
0" - «"
o- - "
o- -
0" - "
0" - "
o- -
0" - "
SAMPLE DEST. /CONTAINER SIZE
PHYS CHEN MBIO OTHER
D
D
D
D
D
D
D
D
D
D
SAMPLE
METHOD
5
S
S
ft
S
S
6
5
S
S
CHEST
NO.
SAMP
TYPE
S
S
S
S
S
S
S
S
S
PRES-
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TIME
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
TEST DESCRIPTION \ NOTES
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATIOH
MICRO BIOLOGICAL EVALUATION
K>
SAMPLE CONTAINER DESCRIPTION:
A - 1 Gal Aab Jug (4 It)
B - 1 0«1 Jar (4 It)
C - 1/2 Gal Jar (2 It)
D - 1 Pt A»b Jar (450*1)
E - 1/2 Pt Aab Jar (250al)
W - VOA Vial
SAMPLE METHODi
1 Dlaturbed
2 Undisturbed
3 Drill
4 Backhoa
S Irowal
Bailer
7 Pu»p
Hand Core
Split Spoon
10 Grab
}1 Coapoelte
12 Hand Auger
SAMPLE TYPE:
s-soll
M-Nater
0-011
X-Other (deecrlbe)
-------
s
8s
i!
M SIZE
OTHER
1
H in
i§
ii
O H
X M
M
si
U
i
i
»
"§
H cn
'I
o
M
X M
X M
< H
22
SM
0?
-2
M
H O
2£
!l
M
X M
g15
0?
-2
"I
-1
"2
8
b
U
'*
» *< < JB
I O « V
I « X O O
i I I I I
MS OK
O (
0 « «
b & w O
O M "3
U 4
9
A.
c« e
Gkt. O
X M O OS
O » «
I h) *< O M
I 9 »*
I *» « M M S
i «-i *
I
1
b *
It
i i i i
161014/1-FINAL REPORT
L-27
-------
/
4
\
3
\
2
.\
1
C N I M
CH0342
CH0343
'CH0344
CM0339
CH0340
CH0341
CH0336
CH0337
CH0338
CN0333
CH0334
CH033S
CH0354
CH0355
CH0356
CH0351
CH03S2
CH0353
CH0348
CH0349
CH03SO
CH0345
CH0346
CH0347
C N
NOTES:
1. REMOVE 3 RANDOM SAMPLES MOM f ACM
QUADRANT AND COMPOSITE.
2. SAMPLES SHOULD BE REMOVED PROM THE
TREATMENT ZONE (TOP ')
3. SAMPLES SHOULD BE REMOVED FROM THE
CENTRAL 10' OF EACH TREATMENT LANE.
CH0366
CH036T
CH0368
CH0363
CH0364
CH0365
CH0360
CH0391
CH0362
CH0357
CH03S*
CH03S*
CN037S
CH037*
CH03IO
CH037S
CH0376
CH0377
CH0372
CH0373
CH0374
CH03CB7
CHOSit
CH0370/
CH03IS
CH0371
1 M
»
* «
«
m
««
29
&
22
15
8
FIELD BLANKS
CH03M
CH0313
1
POST
::ttJi MUO WTt TASK FORCE
Zr*** MIO PMOCESS AREA
nntS SAMPUNO AREA
«*** ARRIC 30. 1»t7
£ C 0 V A JM B^P^^tfu-A-oie
610U/1-FINAL REPORT
L-30
-------
SAMPLE LOCATION SUMMARY
90
M
S
PROJECT NAME
RIO TASK rORCE\
MONSANTO
PROJECT NUMBER
61014
CONDITIONS
Paj>«_6 Of __ -
N\A
PIT/BORINO NO. N\A
LOCATION
BRIO REFINERY SITE ENCLOSURE
SAMPLE DESIGN BY
ROSS\LAAXSO
DATE 3\25\«7
TIME 9:00 - 12:30
SAMPLE
NUMBER
CHI 141
CHM42
CH1143
CH1144
CH1145
CM114C
1
DEPTH
0" - «"
0" - 6"
0" - «"
0" - «"
o- - "
0" - "
SAMPLE DEST. /CONTAINER SIZE
PNTS CHEN MBIO OTHER
D
D
D
D
D
D
SAMPLE
METHOD
9
9
S
5
5
S
CHEST
MO.
SAMP
TYPE
S
S
S
S
S
S
PRES-
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TIME
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
TEST DESCRIPTION. \ NOTES
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
K»
O
SAMPLE CONTAINER DESCRIPTIONi
A - 1 Oml ABb Jug (4 It)
B - 1 Gal Jar (4 It)
C - 1/2 0*1 Jar (2 It)
D - 1 Pt Aab Jar (4SOal)
SAMPLE METHODi
I DUturb«d
2 Undlaturbad
3 Drill
4 Baekho*
iTrowal
7 Pu*p
B Hand Cor*
Split Spoon
10 Orab
11 Coapoalte
12 Hand Aua«r
SAMPLE TYPE:
S-Soll
M-Hat«r
0-011
X-Othar (deacrlba)
-------
in
o
u
a.
\t\
I
Ul
8 8
In
2 I 9
ii
ii
Tf
§
08
"2
2-
52
E
I
SS
ii
i
ii
w
I
:i
ii
*:
li
M i
i - >.
»::§
3SS3S
8" S
lii
~J> 6>V
M C
* O« b
I I I I I I I I I
I 4 O a M * O * »
161014/1-FINAL REPORT
L-32
-------
fl
BM]
Z
f
9d
w
w
O
SAMPLE LOCATION SUMMARY
Pag._
of
PROJECT MM*
BRIO TASK rOMCIX
MONSANTO
PROJECT NUMBER
1014
CONDITIONS
N\A
PIT/BORINO HO.
MXA
LOCATION
MIO REFINERY SITE ENCLOSURE
SAMPLE OKSIOK BY ROSSXLAAEBO
OATt
4\30\«7
Tim
OViOO - 11:30
SAMPLE
CR0333
CB039S
CH033*
CH0342
CM0349
1
CH034S
CH03S1
CH03S4
CH03S7
CH03«0
DEP1H
0- -
O" -
0" -
0" -
o* -
o- -
0" -
o- -
0" - "
0" - «"
AMPLE
B*ST . /CONTAIN
Clfltt MBXO
I
I
I
I
I
I
I
I
I
I
at six*
OTHER
SAMPLE
METHOD
*
*
§
*
*
'
*
*
S
9
CHEST
MO.
SAMP
TYMl
f
"
S
PRES-
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
MM
RJliD
TIME
10
DATS
10
DATS
10
DATS
10
DATS
10
DATS
10
DATS
10
DATS
10
OATS
10
DATS
10
DATS
TEST DESCRIPTION X MOTES
TOTAL OROARIC CARBON.
BASB HEOTRAL ACID EXTRACTABLtSXVOLATXLES
TOTAL OROANIC CARBON.
BASE MEOTRAL ACID EXTRACTABLBSXVOLATILES
TOTAL OROANIC CARBON.
BASB MEOTRAL ACID KXTRACTASLBSXVOLATILBS
TOTAL OROANIC CARBON,
BAS* NEOTRAL ACID BXTRACTABLKSXVOLATILKS
TOTAL OROAHIC CARBON,
BAS* NEOTRAL ACID EXTRACTABLESXVOLATILES
TOTAL OROANIC CARBON.
BAS* NEOTRAL ACID BXTRACTABLESXVOLATILES
TOTAL OROANIC CARBON.
BASB NEOTRAL ACID EXTRACTABLESXVOLATILES
TOTAL OROANIC CARBON.
BASB NEUTRAL ACID EXTRACTABLESXVOLATILES
TOTAL OROAHIC CARBON,
BASK NEUTRAL ACID EXTRACTABLESXVOLATILES
TOTAL OROANIC CARBON.
BASE NEUTRAL ACID EXTRACTABLESXVOLATILES
SAMPLE CONTAINER DESCRIPTION:
I 0*1 Aab Jug M lt|
1 Oal Jar (4 It}
1/2 Oal Jar (I It)
I Pt Anb Jar (4SO»1|
1/J Pt Aab Jar |290«1)
V0» Vial
a - " Car*
SAMPLE METHOD!
1 Dtaturbad
2 Undlaturbcd
3 Drill
4 Backho*
5 Trowel
Ballar
7 Piwp
Hand Cor*
» Split Spoon
10 Grab '
11 CoBpoalta
12 Hand Auger
SAMPLE TYPE:
S-Soll
H-Hater
0-011
X-Oth«r (deacribv)
-------
I
2
z
r
»
M
o
»
H
SAMPLE LOCATION SUMMARY
MIO TMK fORCBV
PROJECT NUMBER
1014
COKDITIONS
Pag« 4 of 6_ _
N\A
PIT/BORINO M.
N\A
LOCATION
MIO REPINERY SITI IMCLOSORB
SAMPLE DESION BT
ROSSXLAAESO
BATS 4\30\«7
TIKI
0«:00 - 11:30
SAMPLE
OOM4
CI03S7
00970
CM373
,CMS7«
C*JM7t
ORWSSt
cao3«4
DEPII
0" -
o- -
o- -
o- - "
o* -
0" -
e- - «
e- -
JkNKB MST./COMTAinft «IZt
pm CON mio oraui
t
i
i
t
i
i
i
i
SAMTU
NRIOO
t
0.
«AMT
TTVB
S
S
rus-
BKVAT
CIILL
CHILL
CIILL
CIILL
CIILL
MILL
CIILL
CIILL
TINS
10
OATS
10
DAYS
10
DATS
to
DATS
to'
DATS
10
DATS
10
DATS
10
DATS
TEST DKSCKIPTIOM \ MOTES
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS i SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS! SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS: SOLUBLE AMMONIUM,
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS: SOLUBLE AMMONIUM,
NITRATE. PHOSPHOROUS DUPLICATE SAMPLE
INOROANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS PIELD BLANK
SAMPLE CONTAINER DESCRIPTION:
- 1 Oal Aab Jua (4 It)
- 1 Oal Jar (4 It)
- I/a Oal Jar (I It)
- 1 Pt A»b Jar (450*11
- 1/8 Pt A«b Jar (250«1)
- VOA Vial
- " Cor*
N - Whirl Pack
I - | qt A«b Jar (»50»1)
SAMPLE METHOD:
1 Olaturbad
a Undisturbed
S Drill
4 Backho*
9 Trowel
Ballar
7 Pu»p
Hand Cor*
Split Spoon
10 Grab
11 Co>poalt*
ia Hand »ug«r
SAMPLE TYPE:
S-Soll
H-Hat«r
O-O11
X-Oth*r (dcacrlbe)
-------
z
>
r*
»
w
«
O
SAMPLE LOCATION SUMMARY
Page.
PROJECT NAME
RIO TASK PORCBN
MOMANTO
PROJECT NUMBER
1014
CONDITIONS
N\A
PIT/BORING NO. N\A
LOCATION
RIO REPINERT SITE ENCLOSORE
SAMPLE DRSION ET
ROSSVLAAESO
DATE
4\JO\S7
TINE OtiOO - 11(30
SAMPLE
CH0334
CN0337
CH0340
CN0343
CHOS4S
CH034*
CHASM
CH03SS
CH03SS
CH03«1
UU II
O" -
0" - *
0" - t"
o- - -
o- -
o- -
o- - s»
0" -
0" - S"
0" -
SAMPLE
PHTS
DEBT. /CONTAINER SXEE
OEM MBIO OTEER
Z
I
I
I
I
I
I
I
I
I
SAMPLE
METHOD
*
'
*
'
*
S
4
S
CHEST
NO.
SAMP
TTPE
S
$
S
s
s
s
s
PRES-
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
OLD
TIME
to
DATS
to
DATS
10
DATS
10
DATS
10
DATS
10
DATS
to
DATS
10
DATS
10
DATS
10
DAYS
TEST DESCRIPTION \ NOTES
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS t SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS i SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS i SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INOROANIC NUTRIENTS i SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS i SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
INORGANIC NUTRIENTS: SOLUBLE AMMONIUM.
NITRATE. PHOSPHOROUS
SAMPLE CONTAINER DESCRIPTION:
A - 1 0*1 Aab Ju0 (4 It)
B - 1 Gal Jar 14 It}
C - 1/2 Gal Jar O It)
0 - I Pt A«b Jar (4SO«J)
E - I/a Pt kmb Jar (J50«l|
p - VOA vial
SAMPLE METNODl
O - "
Car*
-« 0...1.
Disturbed
Undisturbed
Drill
Baekhaa
Trowel
Ballar
7 Puap
Hand Cor*
Spilt Spoon
10 Grab
11 Conpoclt*
It Hand Auger
SAMPLE TYPE.
S-Soll
M-Hatar
O-O11
X-Oth*r (deacrlba)
-------
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SAMPLE LOCATION SUMMARY
z
>
r-
»
PI
O
MIO TASK rORCES
PROJECT NUMBER
1014
CONDITIONS
Paaa of _
N\A
PIT/WWIIM M.
N\A
LOCATION
BRIO REPINIRT SIT! ENCLOSURE
MTU DESION BY ROSSNLAAKSO
DATE
4\30\«7
TIME OS:00 - 11:90
SAMPLE
CW3SB
CW9CB
CM1TI
CH0174
CNO377
CHO3«0
DKPTR
0" -
o- _ *
o- -
o- -
o- -
o- -
SAMTtl DEST. /CONTAINER SIZE
NT* OEM MUO OTHER
0
0
D
D
0
0
SAMPLE
METHOD
6
S
S
>
CHEST
HO.
SAMP
TYPE
S
S
S
S
S
S
PRES-
ERVAT
CHILL
CHILL
CHILL
CHILL
CHILL
CHILL
HOLD
TIME
to
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
10
DAYS
TEST DESCRIPTION \ NOTES
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOOICAL EVALUATION
MICRO BIOLOOICAL EVALUATION
MICRO BIOLOOICAL EVALUATION
MICRO BIOLOGICAL EVALUATION
MICRO BIOLOOICAL EVALUATION
SANK* CONTAINER DESCRIPTION i
A - 1 Oal Aab Ja0 (4 It)
- 1 0*1 Jar 14 It)
O - I/a Sal Jar (a It)
D - 1 Pt ABB Jar <4»O»1)
- I/a Pt A»b Jar (180al)
P - VGA Vial
O - " Cora
H - Whirl Pack
I - 1 Qt Aab Jar (»50al)
SAMPLE METHODi
1 Dlaturbad
a Undiaturbad
* Drill
4 Backhoa
S Trowal
Ballar
7 Puap
Hand Cora
Split Spoon
10 Orab
11 Coapoalta
12 Hand Auger
SAMPLE TYPEi
S-Soll
N-Natar
0-011
I-Othar (daacrlba)
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z
w
O
SAMPLE LOCATION SUMMARY
PROJECT MANE
MIO TASK PORCEX
MONSANTO
PROJECT NUMBER 1014
eOHOXTIOMS
Pag* 5 of
H\A
PIT/BORIM MO. M\A
LOCATION BRIO RIPIMERY SITE ENCLOSURE
SAMPLE MS10M IT
OSSMJUUCSO
DATS
4\30\B7
TINE Off:00 - 11:30
SAMPUI
NUMBER
CH033S
CN033*
CM0341
£0344
CM0341
0«03»0
omasa
CN03M
CH03SS
OM03«I
DEPTH
0* - "
0" -
o- - «
o- -
o- -
0" - "
0" - "
0" -
0" -
o- -
SAMPLE DEBT. /CONTAINER SIZE
PITS CBEN MSIO OTRER
0
0
0
D
D
D
D
0
D
D
SAMPLE
HETEOD
S
S
CEEST
MO.
SAMP
IIPE
S
S
8
S
S
PRES-
ERTAT
CHLt
CRILL
CRILL
CRILL
CHILL
CEIU,
OKU.
CBILL
CHILL
CHILL
TIME
to
OATS
10
OATS
10
OATS
10
DATS
10
DATS
10
OATS
ao
DATS
10
DATS
10
DATS
10
OATS
TEST DESCRIPTION \ MOTES
MICRO BIOLOGICAL EVALUATION
MICRO RIOLOOICAL (VALUATION
MICRO BIOMMICAL EVALUATION
MICRO BIOLOOICAL EVALUATION
MICRO BIOLOOICAL EVALUATION
MICRO BIOLOOICAL EVALUATION
MICRO BIOLOOICAL EVALUATION
MICRO BIOLOOICAL EVALUATION
MICRO BIOLOOICAL EVALUATION
MICRO BIOLOOICAL EVALUATION
SAMPLE CONTAINER DESCRIPTION i
A - 1 0*1 ABb Ju0 (4 It)
D - 1 9*1 J«r l« It)
C - 1/2 0*1 Jar (J It)
D - I Pt A»b Jar (450«l|
E - I/a Pt Aab Jar (ISO»1(
P - VOA Vial
0-6" Cor*
H - Whirl Pack
t - i nt *>b Jar 1950ml)
SAMPLE METHODi
1 Dlaturb*4
2 Undl«tarb*d
3 Drill
4 Backho*
S Trow«l
8*1J«r
1 Pu»p
Hand Cor*
Split Spoon
10 Orab
11 Coapo»lt*
12 Hand Auger
SAMPLE TTPEi
S-Soll
N-Hat*r
O-OI1
X-Oth*r |de>crlbe)
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