EPA-600/3-78-008
January 1978
Ecological Research Series
          *"  ''"*' ~4> f' - <    % •»'

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                RESEARCH REPORTING SERIES

Research reports of the Office of Research and Development, U S  Environmental
Protection Agency, have been grouped into nine series  These nine broad cate-
gories were established to facilitate further development and  application of en-
vironmental technology  Elimination of traditional grouping  was  consciously
planned to foster technology transfer and a maximum interface in related fields
The nine series are

      1.  Environmental  Health Effects Research
      2  Environmental  Protection Technology
      3  Ecological Research
      4  Environmental  Monitoring
      5.  Socioeconomic Environmental Studies
      6  Scientific and Technical Assessment Reports (STAR)
      7  Interagency Energy-Environment Research and Development
      8  "Special" Reports
      9.  Miscellaneous Reports

This report has been assigned to the ECOLOGICAL RESEARCH series This series
describes research on the effects of pollution on humans, plant and animal spe-
cies, and materials. Problems  are assessed for their long- and short-term influ-
ences  Investigations include formation, transport, and pathway studies to deter-
mine the fate of pollutants and their effects This work provides the technical basis
for setting standards to minimize undesirable changes in living organisms in the
aquatic, terrestrial, and atmospheric environments.
This document is available to the public through the National Technical Informa-
tion Service, Springfield, Virginia 22161.

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                                                EPA-600/3-78-008
                                                January 1978
     SUMMARY ANALYSIS OF THE NORTH AMERICAN  (US PORTION)
OECD EUTROPHICATION PROJECT:  NUTRIENT LOADING - LAKE RESPONSE
            RELATIONSHIPS AND TROPHIC STATE  INDICES
                             by

                        Walter Rast
                             and
                        G. Fred Lee
             Center for Environmental Studies
            The University of  Texas  at  Dallas
                 Richardson,  Texas 75080
               Contracts No. R-803356-01-0
                  and No. R-803356-01-3
                    Project Officers
        Norbert Jaworski and Jack H. Gakstatter
      Corvallis Environmental Research Laboratory
                Corvallis, Oregon 97330
      CORVALLIS ENVIRONMENTAL RESEARCH LABORATORY
          OFFICE OF RESEARCH AND DEVELOPMENT
          US ENVIRONMENTAL PROTECTION AGENCY
               CORVALLIS,  OREGON 97330

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                           DISCLAIMER
     This report has been reviewed by the Corvallis Environmental
Research Laboratory, U.S. Environmental Protection Agency,  and
approved for publication.  Approval does not signify that the con-
tents necessarily reflect the views and policies of the U.S.  En-
vironmental Protection Agency, nor does mention of trade names
or commercial products constitute endorsement or recommendation
for use.
                   '7
                               ii

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                                  FOREWORD


           Effective regulatory and enforcement actions by the Environ-
      mental Protection Agency would be virtually impossible without
      sound scientific data on pollutants and their impact on environ-
 %     mental stability and human health.  Responsibility for building
 '     this data base has been assigned to EPA's Office of Research
      and Development and its 15 major field installations, one of
 »     which is the Corvallis Environmental Research Laboratory (CERL).

           The primary mission of the Corvallis Laboratory is research
 ^     on the effects of environmental pollutants on terrestrial, fresh-
      water, and marine ecosystems; the behavior, effects and control
 ,     of pollutants in lake systems; and the development of predictive
'v"     models on the movement of pollutants in the biosphere.
<-o
^          This report provides an extensive examination of relation-
fx.     ships between nutrient inputs and lake responses and, therefore,
»     should be extremely valuable to those people concerned with lake
      management and controlling accelerated lake eutrophication.
                                                A.F. Bartsch
                                                Director, CERL
                                      111

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                            PREFACE

     Several years ago the Organization for Economic Cooperation
and Development (OECD) member countries, including the USA,  ini-
tiated a eutrophication study with the primary objective of  formu-
lating the relationships between aquatic plant nutrient loadings
to lakes and impoundments and the response of these water bodies
to these loadings.  Emphasis was on the development of relation-
ships that could be used to identify critical aquatic plant
nutrient (i.e. nitrogen and phosphorus) loadings in order to avoid
or minimize water quality problems caused by excessive fertiliza-
tion (eutrophication).  In the majority of the participating
countries, the OECD eutrophication study caused the initiation of
field studies, using the same or similar sampling techniques and
analytical methods, to assess aquatic plant nutrient loadings
to a water body and its response to these loadings.  In the  US,
however, the lack of funds to initiate comparable studies of US
water bodies limited the United States' participation in the
overall study.  The US EPA did, however, provide small grants to
enable investigators who had already conducted nutrient load-
response studies in US water bodies to develop a report of their
studies which emphasized nutrient load-lake response relationships
in accord with overall OECD Eutrophication Program objectives and
format.  Funds were also provided by the US EPA to prepare this
summary report.  This report represents an initial analysis  of
the results of the US portion of the North American Project  of
the OECD eutrophication study.

     The goal of the OECD eutrophication study is the quantifica-
tion of the relationships between nutrient loading and trophic
response in lakes and impoundments.  Attention in this initial
analysis has been focused mainly on evaluation of the nutrient
loading portion of this relationship, especially as these nutrient
loadings are related to the critical nutrient loading levels
and the trophic response of the US OECD water bodies, using  the
Vollenweider phosphorus and nitrogen loading diagrams.  This re-
port also evaluates the nutrient sources, nutrient budget calcula-
tion methodologies, and nutrient loading estimates reported  by
the US OECD investigators for their respective water bodies.
The US OECD water body nutrient loadings have been evaluated
several ways, including: (1) several relationships developed by
Vollenweider, (2) comparison with calculated nutrient loadings
based on watershed nutrient export coefficients and land usage
patterns within the watershed, and (3) other nutrient loading-
                                 IV

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lake response relationships developed by Vollenweider,  Dillon,
and Larsen and Mercier.  In addition, an attempt was made in this
summary report to formulate some of the relationships between
nutrient load-lake and impoundment water quality responses,
based on the data available for the US OECD water bodies.

     This report also presents a discussion of the application
of the US OECD eutrophication study results for predicting the
changes in water quality that will arise from altering  the phos-
phorus input to lakes and impoundments.   The US OECD water bodies
are ranked in accord with various previously proposed trophic
status index systems.  A new trophic status index system based
on a modification of the Vollenweider phosphorus loading relation-
ships is presented.   A modified Vollenweider phosphorus loading
relationship has been developed which enables individuals con-
cerned with water quality management to select the appropriate
phosphorus loadings  for achieving a desired level of chlorophyll,
water clarity, and hypolimnetic oxygen depletion rate.

     Upon completion of this study a copy of those sections of
the report pertinent to each investigator's water bodies was sent
to the investigators and a request was made for them to  review
and comment on these sections.  Approximately half of the US
OECD eutrophication study investigators responded to this request,
In the two years from the time that the US OECD eutrophication
investigators had provided the data which served as the basis
of this report and the completion of this report, several in-
vestigators have done additional work on their respective water
bodies.  The new data was brought to the authors' attention as
part of the review process.  In most cases the changes  in the
data were relatively minor and did not change the conclusions
of the report.  In others, major changes in the nutrient loads
for their water body were reported, under conditions where the
investigator indicated that the new data more reliably
estimated   the nutrient loads and should be used instead of the
ones reported previously.

     All suggested changes of the investigators have been
noted in this report and in the appendices.  Major changes have
been used as a basis for rewriting sections of this report.
This situation will cause differences between the data presented
in the investigator's report published as a companion volume,
and the data presented in this report.
                                v

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                            ABSTRACT

     The US participation in the OECD Eutrophication Program
consisted of having 20 investigators prepare reports on the
nutrient load-lake and impoundment response relationships for
their respective water bodies.   This report presents a critical
review of these overall relationships with particular emphasis
given to evaluation of the Vollenweider nutrient load-trophic
state formulations.  This review includes consideration of the
nutrient load response relationships for 38 water bodies, or
parts of water bodies, located  throughout the US, with the pre-
ponderance located in the northern half of the US.  It has been
found that the Vollenweider nutrient load relationship involving
water body mean depth, hydraulic residence time and phosphorus
load correlates well with the trophic states assigned by the US
OECD eutrophication study investigators.

     A good correlation has also been found between phosphorus
loading, normalized as to hydraulic residence time and mean
depth, and the average chlorophyll and water clarity (as measured
by Secchi depth) for the US OECD water bodies.  In general,
phosphorus and nitrogen loads to US OECD water bodies were within
a factor of + two of the loads  predicted on the basis of average
nutrient concentrations within the water bodies and on the land
use patterns within the water body watersheds.  Generalized
nutrient export coefficients have been developed in this study,
enabling estimates of nutrient  loads to be made on the basis
of land use patterns within the watershed.

     The relationships developed in this study can be used to pre-
dict the improvement in water quality that will result from a
change in the phosphorus load to a water body for which phos-
phorus is the key chemical element limiting planktonic algal
growth.  The US OECD water bodies all show approximately the
same trophic status when evaluated by several recently-proposed
trophic state index systems.  A new trophic state index system
has been developed in this study which is based on the relation-
ship between the actual phosphorus loading and permissible phos-
phorus loading as defined in the Vollenweider phosphorus loading
and mean depth/hydraulic residence time relationship.  This
relationship has been modified to enable water quality managers
to determine the appropriate phosphorus load for a particular
water body in order to yield a certain chlorophyll content from
planktonic algae and its corresponding water clarity.  It  is
recommended that these relationships be used as a basis for

                                vi

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establishing critical phosphorus loads to lakes and impoundments.

     This report was submitted in fulfillment of Contract No.
R-803356-01-0 and Contract No. R-803356-01-3 under the sponsor-
ship of the U.S. Environmental Protection Agency.  Work was
completed as of August, 1977.
                                VII

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                             CONTENTS

Foreword 	    iii
Preface 	    iv
Abstract	    vi
Figures 	   xii
Tables  	xviii
Acknowledgment 	   xxi

   I.    Introduction 	     i
   II.  Conclusions  	     3
   III. Recommendations 	     6
   IV.  Organization for Economic Cooperation and
        Development  	     8
           Water Management Sector Group  	    10
           OECD International Cooperative Program
           for Monitoring of Inland Waters  	    12
              Objectives of Study 	    12
              Common Measurement System  	    13
              Regional Approach  	    13
   V.    US OECD Eutrophication Study 	    18
           General Characteristics of US OECD Water
           Bodies  	    23
           Data Reporting Methodology  	    30
           US OECD Eutrophication Study and Other
           US Eutrophication Control Programs 	    31
              National Eutrophication Survey 	    31
              Public Law 92-500  	    31
           Use of N:P Ratios in Determining the Aquatic
           Plant Growth Limiting Nutrient in Natural Waters...    32
              The Limiting Nutrient Concept 	    32
              Nitrogen and Phosphorus as Limiting Nutrients  ..    33
              Interaction Between Biotic and Abiotic
              Factors in Determining Limiting Nutrient
              and Algal Nutrient Stoichiometry  	    36
              The Limiting Nutrient Concept as Applied in
              The US OECD Eutrophication Study  	    39
              Aquatic Plant Limitation in US OECD Water
              Bodies 	    47
           Approaches Used in US OECD Eutrophication
           Study 	    50
              Initial Vollenweider Phosphorus and
              Nitrogen Loading Diagrams  	    50
              Vollenweider Phosphorus Loading and
              Nitrogen Loading and Mean Depth/Hydraulic
              Residence Time Relationships  	    55

                                viii

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           Emphasis on Phosphorus Loading
           Relationship 	 63
           Vollenweider Critical Phosphorus Loading
           Equations 	 63
           Vollenweider Phosphorus Loading Characteristics
           and Mean Epilimnetic Chlorophyll  a Relationship.. 67
           Dillon Phosphorus Loading-Phosphorus
           Retention and Mean Depth Relationship 	 70
           Larsen and Mercier Influent Phosphorus
           and Phosphorus Retention Relationship 	 74
VI.  Results of the Initial Analysis of US OECD
     Eutrophication Study Data 	 79
        Sampling and Measurement Methodologies 	 79
        Nutrient Load Calculation Methodologies  	 80
        Methods for Evaluation of Estimate of US
        OECD Water Body Nutrient Loadings  	Ill
           Vollenweider Mean Phosphorus/Influent
           Phosphorus and Hydaulic Residence
           Time Relationship 	118
           Watershed Land Use Nutrient Export
           Coefficients 	125
           Comparison of Phosphorus Loadings Derived
           From Vollenweider Relationship with
           Loadings Derived From Watershed Phos-
           phorus Export Coefficient 	139
VII. US OECD Eutrophication Study Phosphorus Data:	147
        As Applied in Initial Vollenweider Phosphorus
        Loading and Mean Depth/Hydraulic Residence
        Time Relationship; 	'	147
        As Applied in Modified Vollenweider
        Phosphorus Loading Mean Depth/Hydraulic Residence
        Time Relationship; 	153
        As Applied in Phosphorus Residence Time
        Model; 	160
        As Applied in Vollenweider Equation for
        Critical Phosphorus Loading 	169
        Comparison of Results :  	170
           Discrepancies Between Vollenweider
           Phosphorus Loading Diagram and
           Vollenweider Mean Phosphorus/Influent
           Phosphorus and Hydraulic Residence
           Time Diagram 	175
              Lake Waldo 	.-	175
              Lake Weir 	177
              Lower Lake Minnetonka 	178
              Twin Lakes - 1973  and 1974 	179
              Potomac Estuary and Lake  of the Isles  	180
              Lake Stewart, Lake  Virginia and
              Twin Valley Lake  	181
              Kerr Reservoir 	183
           Discrepancies Between  Vollenweider
           Phosphorus Loading Diagram and Watershed
           Phosphorus Export Coefficient Calculations	184

                              ix

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               Dogfish Lake,  Lamb Lake and
               Meander Lake 	134
               Lake Tahoe 	185
               Lake Sallie 	186
VIII. US OECD Eutrophication  Study Nitrogen Data:	187
         As Applied in Vollenweider Nitrogen Loading
         and Mean Depth/Hydraulic Residence Time
         Relationship 	 187
         Comparison of Results :
            Discrepancies Between Investigator-
            Indicated Nitrogen Loadings and Water-
            shed Nitrogen Export Coefficient
            Calculations	190
               Lake Sallie 	191
               Lake Tahoe 	,	191
               Lake Sammamish, Lake Cayuga and '
               Twin Lakes 	192
 IX.   US OECD Data Applied in Other Nutrient
      Relationships 	 193
         US OECD Phosphorus Data Applied in
         Vollenweider's Phosphorus Loading Character-
         istics and Mean Chlorophyll Relationship 	 193
         US OECD Phosphorus Data Applied in
         Phosphorus Loading and  Secchi Depth
         Relationship 	 201
         US OECD Phosphorus Data Applied in Dillon's
         Phosphorus Loading-Phosphorus Retention
         and Mean Depth Relationship 	202
         US OECD Phosphorus Data Applied in Larsen
         and Mercier's  Influent  Phosphorus and
         Phosphorus Retention Relationship 	 211
 X.    Correlations Between Nutrient Loadings and
      Eutrophication Response Parameters 	 217
         Phosphorus Loadings  	 221
         Nitrogen Loadings 	 232
         Mean Total and Dissolved Phosphorus
         Concentrations	244
         Mean Inorganic Nitrogen Concentrations 	 262
         Other Correlations Between Eutrophication
         Response Parameters  	 267
 XI.   Application of US OECD  Results for Predicting
      Changes in Water Quality as a Result of Altering
      Nutrient Inputs 	 281
         Application of Results  for Assessing Water
         Quality in the Great Lakes and Impoundments 	 297
         Application of Results  to Implementation of
         Section 314-A of PL  92-500 	311
         An Approach for the  Use of the Vollenweider
         Nutrient Load-Water  Quality Program  	 314
 XII. Trophic Status Index Study 	 320
         General Considerations  	 ^20
         Requirements for a Trophic Status
         Classification Index 	 323

                              x

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           Current Trophic Status Classification
           Indices	 324
              US EPA Trophic Status Index System 	 324
              Carlson Trophic Status Index System 	 326
              Piwoni and Lee Trophic Status Index System  	 328
              Rast and Lee Trophic Status Index Systems 	 330
           Trophic Status Indices as Applied to the
           US OECD Water Bodies  	 335
              US EPA Trophic Status Index System 	 335
              Carlson Trophic Status Index System 	 342
              Piwoni and Lee Trophic Status Index System  	 348
              Rast and Lee Trophic Status Index Systems 	 348
   XIII .  Discussion 	 368

References 	 380
Appendices

   I.    US OECD Final Report Outline 	 400
  II.    Data Summary Sheets for US OECD
        Water Bodies 	 403

Glossary 	 453
                                  XI

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                             FIGURES


Number                                                       Page

  1   Organizational Structure of OECD .....................    9

  2   Organizational Structure of OECD Environment
      Committee ............................................   11

  3   Organizational Outline of OECD Eutrophication Study . .   16

  4   Locations of US OECD Water Bodies ....................   22

  5   Vollenweider ' s Total Phosphorus Loading and Mean
      Depth Relationship  ...................................   52

  6   Vollenweider ' s Total Nitrogen Loading and Mean
      Depth Relationship  ...................................   53

  7   Initial Vollenweider Total Phosphorus Loading and
      Mean Depth/Hydraulic Residence Time Relationship  .....   58

  8   Modified Vollenweider Total Phosphorus Loading and
      Mean Depth/Hydraulic Residence Time Relationship  .....   62

  9   Vollenweider Critical Phosphorus Loading and Mean
      Depth Relationship  ............... • ....................   68

 10   Vollenweider Critical Phosphorus Loading and
      Hydraulic Loading Relationship  ..................... . .   69

 11   Vollenweider Phosphorus Loading Characteristics and
      Mean Chlorophyll a  Relationship  ......................   71
 12   Dillon Phosphorus Loading-Phosphorus Retention and
      Mean Depth Relationship
 13   Larsen and Mercier Influent Phosphorus and
      Phosphorus Retention Relationship  ....................   78

 14   Evaluation of Estimates of US OECD Water Body Nutrient
      Loadings:  Vollenweider Mean Phosphorus/Influent
      Phosphorus and Hydraulic Residence Time Relationship..  124

                                 xii

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Number                                                        Page

 15   Evaluation of Estimates of US OECD Water Body
      Nutrient Loadings:   Watershed Land Use Phosphorus
      Export Coefficient  Calculations 	 140

 16   Evaluation of Estimates of US OECD Water Body
      Nutrient Loadings:   Watershed Land Use Nitrogen
      Export Coefficient  Calculations 	 141

 17   Comparison of Phosphorus Loadings Derived from
      Watershed Export Coefficients with Loadings Derived
      From Vollenweider Mean Phosphorus/Influent
      Phosphorus and Hydraulic Residence Time Relationship ... 145

 18   US OECD Data Applied to Initial Vollenweider
      Phosphorus Loading  and Mean Depth/Hydraulic
      Residence Time Relationship  	 148

 19   US OECD Data Applied to Modified Vollenweider
      Phosphorus Loading  and Mean Depth/Hydraulic Residence
      Time Relationship 	 154

 20   Comparison of Permissible and Excessive Loading Lines
      in Initial and Modified Vollenweider Phosphorus Loading
      Diagram 	155

 21   US OECD Data Applied to Vollenweider Nitrogen 'Loading
      and Mean Depth/Hydraulic Residence Time Relationship..... 189

 22   US OECD Data Applied to Vollenweider Phosphorus Loading
      Characteristics and Mean Chlorophyll a Relationship 	199

 23   US OECD Data Applied to Phosphorus Loading and Secchi
      Depth Relationship  (Log-Log Scale) 	203

 24   US OECD Data Applied to Phosphorus Loading and Secchi
      Depth Relationship  (Semilog Scale) 	204

 25   US OECD Data Applied to Dillon Phosphorus Loading-
      Phosphorus Retention and Mean Depth Relationship 	206

 26   US OECD Data Applied to Larsen and Mercier Influent
      Phosphorus and Phosphorus Retention Relationship 	212

 27   Phosphorus Loading  and Mean Chlorophyll a Relationship
      in US OECD Water Bodies 	7. '	222

 28   Phosphorus Loading  and Mean Secchi Depth  Relationship
      in US OECD Water Bodies 	223

 29   Phosphorus Loading  and Mean Total  Phosphorus Relationship
      in US OECD Water Bodies 	225

                                xiii

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Number
 30   Phosphorus Loading and Mean Dissolved Phosphorus
      Relationship 	 226

 31   Phosphorus Loading and Primary Productivity
      Relationship 	 227

 32   Phosphorus Loading and Total Primary Production
      Relationship 	 228

 33   Phosphorus Loading and Growing Season Epilimnetic
      Chlorophyll a Relationship  	 229

 34   Phosphorus Loading and Growing Season Epilimnetic
      Total Phosphorus Relationship 	 230

 35   Phosphorus Loading and Growing Season Epilimnetic
      Dissolved Phosphorus Relationship 	 231

 36   Phosphorus Loading and Growing Season Epilimnetic
      Primary Productivity Relationship 	 233

 37   Phosphorus Loading and Spring Overturn Total
      Phosphorus Relationship 	 234

 38   Nitrogen Loading and Mean Chlorophyll a Relationship.... 235

 39   Nitrogen Loading and Mean Secchi Depth Relationship	236

 4-0   Nitrogen Loading and Mean Inorganic Nitrogen
      Relationship 	 237

 41   Nitrogen Loading and Primary Productivity Relation-
      ship 	 239

 42   Nitrogen Loading and Total Primary Production
      Relationship 	 240

 43   Nitrogen Loading and Growing Season Epilimnetic
      Chlorophyll a Relationship  	 241

 44   Nitrogen Loading and Growing Season Epilimnetic
      Inorganic Nitrogen Relationship  	 242

 45   Nitrogen Loading and Growing Season Epilimnetic
      Primary Productivity Relationship 	 243

 46   Nitrogen Loading and Spring Overturn Inorganic
      Nitrogen Relationship  	 245

 47   Mean Total Phosphorus and Mean Chlorophyll a
      Relationship 	7	 246

                                xiv

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Number                                                        Page

 4-8   Mean Total Phosphorus and Mean Secchi Depth
      Relationship 	 247

 49   Mean Total Phosphorus and Mean Dissolved Phosphorus
      Relationship 	 249

 50   Mean Total Phosphorus and Primary Productivity
      Relationship 	 249

 51   Mean Total Phosphorus and Growing Season Epilimnetic
      Chlorophyll a Relationship 	 250

 52   Mean Total Phosphorus and Growing Season Epilimnetic
      Primary Productivity Relationship 	 251

 53   Mean Total Phosphorus and Spring Overturn Total
      Phosphorus 	252

 54   Growing Season Epilimnetic Total Phosphorus and
      Growing Season Epilimnetic Chlorophyll a Relationship... 253

 55   Growing Season Epilimnetic Total Phosphorus and
      Growing Season Epilimnetic Primary Productivity
      Relationship 	 255

 56   Spring Overturn Total Phosphorus and Growing Season
      Epilimnetic Chlorophyll a Relationship 	 256

 57   Spring Overturn Total Phosphorus and Growing Season
      Epilimnetic Total Phosphorus  Relationship 	 257

 58   Spring OVerturn Total Phosphorus and Growing Season
      Epilimnetic Dissolved Phosphorus Relationship 	 258

 59   Mean Dissolved Phosphorus and Mean Chlorophyll a
      Relationship 	7	 259

 60   Mean Dissolved Phosphorus and Primary Productivity
      Relationship 	 260

 61   Mean Dissolved Phosphorus and Spring Overturn
      Dissolved Phosphorus Relationship 	 261

 62   Growing Season Epilimnetic Dissolved Phosphorus  and
      Growing Season Epilimnetic Chlorophyll a Relationship... 263

 63   Spring Overturn Dissolved Phosphorus and Growing Season
      Epilimnetic Chlorophyll a. Relationship 	 264

 64   Spring Overturn Dissolved Phosphorus and Growing Season
      Epilimnetic Dissolved Phosphorus Relationship 	 265

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Number                                                        Page

 65   Mean Inorganic Nitrogen and Mean Chlorophyll a
      Relationship 	 266

 66   Mean Inorganic Nitrogen and Mean Secchi Depth
      Relationship 	 268

 67   Mean Inorganic Nitrogen and Primary Productivity
      Relationship 	 269

 68   Mean Inorganic Nitrogen and Growing Season Epilmnetic
      Chlorophyll a Relationship 	 270

 69   Mean Inorganic Nitrogen and Growing Season Epilmnetic
      Primary Productivity Relationship  	 271

 70   Growing Season Epilimnetic Inorganic Nitrogen and
      Growing Season Epilmnetic Chlorophyll a Relationship.... 272

 71   Growing Season Epilimnetic Inorganic Nitrogen and
      Growing Season Epilimnetic Primary Productivity
      Relationship 	 273

 72   Primary Productivity and Mean Chlorophyll a
      Relationship 	7	 274

 73   Primary Productivity and Mean Secchi Depth
      Relationship 	 276

 74   Growing Season Primary Productivity and Growing
      Season Mean Chlorophyll a Relationship 	 277

 75   Mean Daily Productivity and Mean Chlorophyll a
      Relationship 	 278

 76   Mean Daily Primary Productivity and Mean Areal
      Chlorophyll a Relationship 	 279

 77   Secchi Depth and Chlorophyll a_ Relationship in
      Natural Waters (Linear Scale)"	 289

 78   Secchi Depth and Chlorophyll a Relationship in
      Natural Waters (Log-Log Scale! 	 291

 79   Phosphorus Loading Characteristics and Secchi
      Depth Relationship in Natural Waters 	 292

 80   Phosphorus Loading Characteristics and Hypolimnetic
      Oxygen Depletion Relationship in Natural  Waters 	 298

 81   Phosphorus Loading and Mean Depth/Hydraulic Residence
      Time Relationship as Applied to Hypothetical Water Body
      Under Several Phosphorus Loading Scenarios	 301

                                xvi

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Number                                                        Page


 82   Phosphorus Loading Characteristics and Mean Chlorophyll
      a_ Relationship as Applied to Hypothetical Water Body
      Under Several Phosphorus Loading Scenarios 	 303

 83   Secchi Depth and Mean Chlorophyll a_ Relationship as
      Applied to Hypothetical Water Body Under Several
      Phosphorus Loading Scenarios 	 304

 84   Phosphorus Loading Characteristics and Secchi Depth
      Relationship as Applied to Hypothetical Water Body
      Under Several Phosphorus Loading Scenarios 	 305

 85   Phosphorus Loading Characteristics and Hypolimnetic
      Oxygen Depletion Relationship as Applied to Hypothetical
      Water Body Under Several Phosphorus Loading Scenarios ... 306

 86   Relationship Between Excessive Phosphorus Loads and
      Chlorophyll a in US OECD Water Bodies 	 363

 87   Relationship Between Excessive Phosphorus Loads and
      Excessive Chlorophyll a in US OECD Water Bodies 	 364

 88   Relationship Between Vollenweider Phosphorus Loading
      Diagram, Summer Mean Chlorophyll a and Secchi Depth 	 366
                                 xvn

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                              TABLES


Number                                                        Page

  1   OECD Member Countries ..................................   8

  2   Summary of Essential and Desirable Parameters in
      OECD Eutrophication Study .............................. 14
  3   List of Water Bodies in OECD North American
      Project (US Portion) ................................... 19

  4   Characteristics of US OECD Water Bodies ................ 24

  5   Summary of Aquatic Plant Micronutrient Requirements ..... 33

  6   Demand : Supply Ratios for the Major Aquatic Plant
      Nutrients ............................................... 35

  7   Atomic Ratios of C, N and P Present in Plankton ........ 37

  8   Chemical Composition of Some Algae Erom Ponds and
      Lakes in the Southeastern US ........................... 38

  9   Summary of Limiting Aquatic Plant Nutrients in
      US OECD Water Bodies ................................... 41

 10   Mass Ratios of Inorganic Nitrogen to Soluble
      Orthophosphate in US OECD Water Bodies ................. 43

 11   Analytical Procedures for Major Response Parameters
      Examined in US OECD Eutrophication Study  ............... 81

 12   Summary of Methods Used to Calculate Nutrient Loadings
      for US OECD Water Bodies ............................... 90

 13   Summary of Nutrient Sources Considered in US OECD
      Water Body Nutrient Loading Estimates .................. 112

 14   Identification Key for US OECD Water Bodies ............ 115

 15   US OECD Data for Vollenweider ' s Mean Phosphorus/
      Influent Phosphorus and Hydraulic Residence Time
      Relationship ........................................... 12°

                                xviii

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Number                                                        Page

 16   Typical Values of Watershed Nutrient Export
      Coefficients 	 127

 17   Watershed Nutrient Export Coefficients Used to Check
      US OECD Nutrient Loadings 	128

 18   US OECD Nutrient Loadings Calculated Using Watershed
      Nutrient Export Coefficients 	 130

 19   Comparison of Phosphorus Loadings Derived from
      Watershed Export Coefficients with Loadings Predicted
      by Vollenweider's Mean Phosphorus/Influent Phosphorus
      and Hydraulic Residence Time Relationship 	 142

 20   Phosphorus and Nitrogen Loadings, Mean Depths (z) and
      Hydraulic Residence Times (T )  for US OECD Water
      Bodies 	"	 149

 21   Phosphorus and Nitrogen Residence Times of US OECD
      Water Bodies	 164

 22   US OECD Data Used in Vollenweider's Critical Phosphorus
      Loading Equation 	 171

 23   US OECD Data Applied to Vollenweider's Phosphorus
      Loading and Mean Chlorophyll a Concentration Relation-
      ship 	7	 194

 24   US OECD Data Applied to Dillon's Phosphorus Loading-
      Phosphorus Retention and Mean Depth Relationship 	 207

 25   US OECD Data Applied 'to Larsen and Mercier's Influent
      Phosphorus Concentration and Phosphorus Retention
      Relationship 	 213

 26   List of Correlations Examined In US OECD Water
      Bodies 	 218

 27   Data for Chlorophyll a and Secchi Depth Relationship.... 286


 28   Summary of Data for Hypothetical Water Body  Under
      Several Phosphorus  Load Reduction Scenarios  	300

 29   Summary of Phosphorus Loading Characteristics,
      Chlorophyll a and Secchi Depth  of Hypothetical
      Water Body Under Several Phosphorus Load
      Reduction Scenarios	309
                                  xix

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Number                                                         Page

 30   General Characteristics Frequently Used to Classify
      Water Bodies	  321

 31   US EPA Trophic State Index Parameters	  325

 32   The Carlson Trophic State Index and Its Associated
      Parameters 	  327

 33    Piwoni and Lee  Trophic State  Index Parameters  	  329

 34    Ranking of US OECD Water Bodies  Using  Modified
       US EPA Trophic  State  Index  System 	  336

 35    Relative Trophic  Status Ranking  of US  OECD Water
       Bodies Using  Modified US EPA  Trophic Status Index
       System 	  340

 36    Ranking of US OECD Water Bodies  Using  Carlson  Trophic
       Status Index  System 	  343

 37    Relative Trophic  Status Ranking  of US  OECD Water
       Bodies Using  Carlson  Trophic  Status Index System 	  346

 38    Ranking of US OECD Water Bodies  Using  Piwoni and Lee
       Modified Trophic  Status Index System 	  349

 39    Relative Trophic  Status Rankings of US OECD Water
       Bodies Using  Piwoni and Lee Modified Trophic Status
       Index System  	  353

 40    Ranking of US OECD Water Bodies  Using  Secchi Depth,
       Chlorophyll a,  Excess Chlorophyll a and Excess Phosphorus
       Loading as Ranking Parameters .... 7	355

 41    Relative Trophic  Status Ranking  of US  OECD Water Bodies
       Using Secchi  Depth, Chlorophyll  a, Excess Chlorophyll a_
       and Excess Phosphorus Loading . .7	359
                                xx

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                        ACKNOWLEDGEMENTS

     This study was supported by contract numbers R-803356-01-0
and R-803356-01-3 from the US EPA National Research Laboratory,
Corvallis, Oregon.  N. Jaworski, formerly of that laboratory,
served as contract officer during the majority of the study
period.  J. Gakstatter served as contract officer during the
final phase of this study.  We wish to acknowledge their assis-
tance in this study.  We also wish to acknowledge the assistance
given this study by all of the US investigators in the OECD
Eutrophication Program.

     Special recognition is due R. Vollenweider of the Canada
Centre for Inland Waters who provided the stimulus for the OECD
eutrophication studies, as well as many of the basic ideas
utilized in this study for data examination and formulation into
nutrient load-lake response relationships which can be utilized
for water quality management.

     Several individuals at the University of Texas at Dallas
contributed significantly to the completion of this .report.
Special recognition should be given to D. Canham, J. Hale,E. Meckel,
M. Jaye, A. Jones, L. Lawhorn, G. Max and P. Wernsing.  Substantial
support was given the completion of this report by the Uni-
versity of Texas at Dallas and EnviroQual Consultants £ Lab-
oratories of Piano, Texas.


     This report is essentially the same as the Ph.D.  dissertation
of Walter Rast for The University of Texas at Dallas.
                               xxi

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                          SECTION  I

                         INTRODUCTION
     The  excessive  fertilization  (eutrophication)  of  natural
waters  is  one of the most  significant  causes  of water quality
deterioration in the US  and  in many  other  countries.   This  in-
creasing  eutrophication, resulting principally from the  cultur-
al  activities of man,  is occurring because of the  excessive in-
put  of  aquatic plant nutrients into  water  bodies.   Some  water
bodies  are naturally eutrophic in that they receive sufficient
supplies  of aquatic plant  nutrients, mainly phosphorus and  ni-
trogen, from natural sources  to produce excessive  growths of
algae and  macrophytes.   However, many  of man's activities which
accelerate this transport  of  aquatic plant nutrients  into water
bodies  can greatly  accelerate the eutrophication process.   While
eutrophication may  be  desirable in some water bodies  to  increase
productivity, in general the  eutrophication process is associ-
ated with  water quality  deterioration.   Excessive  algal  or  macro-
phyte growths can result in  a significant  deterioration  of  water
quality,  which can  greatly hinder the  waters' use  for domestic
and  industrial water supplies, for irrigation and  for recreation.
Today eutrophication ranks as one of the most significant causes
of  water  quality problems  in  the US, and it will probably become
of  greater concern  as  other water pollution problems  are allevi-
ated (Lee, 1971).

     While other elements  have occasionally been proposed (Goldman,
1964; Provasoli, 1969; Kerr et_aJ.,  1970;  Schelske  and Stoermer,
1972),  phosphorus and  nitrogen are generally  considered  to  be
the.  major  nutrients controlling or limiting the productivity of
water bodies, and hence the eutrophication process.   Of  these
two  nutrients , the  key element most  often  found limiting aquatic
plant populations is phosphorus (Vollenweider, 1968;  Lee, 1971;
^1973; Vollenweider  and Dillon, 1974).   Furthermore, in many
instances ,  phosphorus  inputs  to water  bodies  are from point
sources such as domestic wastewaters.   By  contrast, large inputs
of nitrogen  are frequently from non-point  (diffuse) sources such
as agricultural runoff, precipitation,  dry fallout  and nitrogen
fixation.   These diffuse sources are usually  more  difficult to
control.   In general,  phosphorus inputs  are often  more amenable
to control  measures than are  nitrogen  inputs  (Vollenweider  and
Dillon, 1974).   Water  bodies  which are  normally nitrogen-limited
can  possibly be made phosphorus-limited  if the phosphorus in-
puts are reduced sufficiently.

                               1

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     Eutrophication control is frequently based on limiting
aquatic plant nutrient inputs, usually phosphorus.  Attempting
to control the eutrophication process by controlling phosphorus
inputs to natural waters is both technically sound and economi-
cally feasible for many water bodies (Lee, 1973; OECD, 1974-a;
Vollenweider and Dillon, 1974).  However, such a strategy re-
quires that the relationships between the phosphorus inputs and
the trophic responses of the aquatic plant populations of a
given water body be understood on a quantitative basis.  Develop-
ment of such an understanding has always been an extremely
difficult problem because the eutrophication process is a complex
physical, chemical and biological phenomenon (Sawyer, 1966;
Fruh et_ a_l. , 1966; Fruh, 1967; Stewart and Rohlich, 1967; Vollen-
weider, 1968; Federal Water Quality Administration, 1969;
National Academy of Science, 1969; Lee, 1971; 1973; Likens,
1972a; US EPA, 1973a).

     It flfas not been possible in the past to quantitatively re-
late the phosphorus loading of a given water body to the result-
ant  aquatic plant related trophic response, as reflected in its
relative degree of eutrophication.  Consequently, the management
of water systems subjected to cultural eutrophication has been
largely subjective.  Extensive, and often expensive, programs
of aquatic plant nutrient removal from domestic wastewaters or
diversion of point source inputs of nutrients have been initiated
in an attempt to alleviate eutrophication problems in lakes and
impoundments.  These programs have no quantitative data on the
expected effects of these programs on trophic response and water
quality in these water bodies.  Clearly, a quantitative method-
ology is required to initiate effective water quality management
with some assurance that the desired results will be attained.

     In an attempt to alleviate this situation, the Organization
for Economic Cooperation and Development  (OECD) member countries
initiated the Cooperative Programme for the Monitoring of Inland
Waters, which was designed to provide quantitative data on the
aquatic plant nutrient load-lake and impoundment response re-
lationships, with particular emphasis on water quality and the
development of approaches to be used for water quality management
of excessive fertility problems.

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                           SECTION II

                          CONCLUSIONS
1.   Based on the initial analysis of the US OECD eutrophication
    study data,  the approach developed and modified by Vollen-
    weider, relating the phosphorus loading of a phosphorus-
    limited water body to its morphological and hydrological
    characteristics, has considerable validity as a method for
    determining  critical phosphorus loading levels and associated
    overall degree of fertility for US lakes and impoundments.

2.   The findings of this initial analysis give considerable sup-
    port to the  recent adoption of the Vollenweider nutrient
    loading-water body fertility response relationship by the
    US EPA as a  basis for establishing phosphorus loading water
    quality criteria.

3.   Initial analysis of the US OECD data indicates the Vollen-
    weider phosphorus critical loading criteria, developed for
    water bodies located in northern temperate climates,  also
    appears to be applicable to warm climate water bodies such
    as those found in the southern and southwestern US.   Addi-
    tional study needs to be done on water bodies in this
    region to confirm this preliminary conclusion.

4.   The Vollenweider phosphorus critical loading criteria, devel-
    oped for planktonic algal responses to phosphorus loadings,
    will likely have to be modified in order to be applicable to
    water bodies whose primary productivity and aquatic plant nui-
    sance problems are manifested mainly in macrophyte and attached
    algal growth.  Modifications of the critical phosphorus load-
    ings will likely be required where the primary problem arising
    from the excessive fertility is domestic water supply water
    quality.  Further, it is possible that the Vollenweider ap-
    proach will not be applicable to impoundments with hydraulic
    residence times in the order of a month or less, and especially
    for those impoundments that show marked stratification of
    inflowing waters during critical growing seasons.

5.   The results  of this study indicate the feasibility of using
    the Vollenweider approach for determining critical nitrogen
    loading levels and trophic state associations for nitrogen-
    limited water bodies.

6.   The similar relative positioning of the US OECD water bodies

                                 3

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     on both the phosphorus loading and nitrogen loading versus
     mean depth/hydraulic residence time diagrams illustrates the
     relatively constant ratio of nitrogen to phosphorus loading
     to water bodies.

 7.  The relationship developed by Vollenweider, between a water
     body's phosphorus loadings and its mean influent phosphorus
     concentration and hydraulic loading, as well as the use of
     watershed land use nutrient export coefficients, appear to
     be effective means for determining the reasonableness of the
     phosphorus and nitrogen loading estimates to a water body.

 8.  The trophic relationships developed by Vollenweider, between a
     water body's phosphorus loading characteristics and its
     mean chlorophyll concentration;  by Dillon, between phosphorus
     loading and phosphorus retention coefficient and mean depth;
     and by Larsen and Mercier, between mean influent phosphorus
     concentration and phosphorus retention coefficient, also
     appear to be potential tools for estimating phosphorus loads,
     average phosphorus content and associated overall degree of
     fertility for many US lakes and impoundments.

 9.  Because of the lack of uniform analytical and sampling method-
     ologies, direct comparisons of eutrophication data between
     the US OECD water bodies must be made with caution.  In
     general, the correlations between phosphorus loading-concen-
     trations and eutrophication response data are better than
     those observed between nitrogen loading-concentration and the
     same response parameters, and support the observations of
     phosphorus-limitation of most of the US OECD water bodies.

10.  The water quality models derived from the relationships be-
     tween phosphorus  loading and chlorophyll a_, phosphorus load-
     ing and Secchi depth and phosphorus loading and hypolimnetic
     oxygen depletion  offer simple,  practical  and  quantitative
     methodologies for assessing the  expected effects of eutroph-
     ication control programs based on phosphorus removal from
     domestic wastewaters and other phosphorus control programs,
     on water quality  in the affected water bodies.

11.  The recently proposed trophic status index systems of the
     US EPA, Carlson, and Piwoni and Lee produce'relatively similar
     trophic rankings for the US OECD water bodies, suggesting
     that their common ranking parameters may equate their trophic
     ranking abilities.

12.  The trophic status index system based on excess phosphorus
     loading and excess chlorophyll a, derived  in this report, offers
     promise as a trophic ranking system based on the phosphorus
     loading and expected water quality responses in water bodies.

13.  The Vollenweider phosphorus loading and mean depth/hydraulic

                                 4

-------
residence time diagram can be related to the common water
quality parameters of chlorophyll a_, Secchi depth and hypo-
limnetic oxygen depletion, based on the relationships between
total phosphorus, chlorophyll a, Secchi depth and hypo-
limnetic oxygen depletion in natural waters.

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                           SECTION III

                        RECOMMENDATIONS
1.   The US EPA and the states should adopt  the  modified  Vollen-
    weider phosphorus load and mean depth/hydraulic  residence
    time relationship for determining the permissible  phosphorus
    loading for phosphorus-limited lakes and impoundments   where
    the primary concern is the impairment of water quality for
    recreational use.  The recently proposed US EPA Quality
    Criteria for Water (US EPA, 1975b) should be modified  to
    include this recent modification of Vollenweider's model,
    as well as the approaches presented by  Dillon, and Larsen
    and Mercier.

2.   The US should continue to actively participate in the  inter-
    national OECD Eutrophication Program data review,  synthesis
    and report preparation.  Such participation is likely  to
    result in a much better understanding of the types of  water
    bodies that obey the modified Vollenweider nutrient  loading
    relationship.

3.   Research funds should be made available at the federal and
    state levels to further investigate the applicability  of the
    Vollenweider nutrient loading relationships for lakes  and
    impoundments located in the southern half of the US  as well
    as for water bodies with high levels of inorganic turbidity,
    color, attached algae and macrophyte, and floating macro-
    phyte water quality problems.  Also, special consideration
    should be given to water bodies with short hydraulic resi-
    dence times and shallow depths and to impoundments which show
    high degrees of stratified inter or underflow waters.

4.   Studies should be conducted to further  refine the permissible
    versus excessive loading criteria, giving particular atten-
    tion to differences in water quality problems associated with
    recreational use in various regions of  the US, especially the
    southern half of the US, and the critical nutrient loadings
    for impairment of domestic water supply water quality.

5.   Further work should be done to establish a relationship be-
    tween the critical phosphorus loading relationship as defined
    by Vollenweider, the actual phosphorus  loading for a given
    water body, and  its associated water quality.  The ultimate

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    objective of these studies should be the development of
    quantitative relationships which can be used to further
    predict a change in the water body's water quality as a
    function of an altered nutrient load.   Particular attention
    should be given to assessment of water quality in terms of
    planktonic algal growth, attached algae and macrophyte
    growth, chlorophyll concentration, water clarity and hypolim-
    netic oxygen depletion.

6.   Studies should be conducted to develop similar nitrogen re-
    lationships and information as described above for phosphorus

7.   Studies need to be conducted to examine the significance of
    utilizing total phosphorus and total nitrogen as a basis
    for establishing loading criteria versus using the available
    forms of these nutrients for establishing loading criteria.

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                            SECTION IV

               ORGANIZATION FOR ECONOMIC COOPERATION
                          AND DEVELOPMENT
     The Organization for Economic Cooperation and Development
(OECD) is an independent, international organization headquartered
in Paris.  It is concerned primarily with the economic growth of
its twenty-four member nations.  These comprise the more highly
developed countries of the world, excluding the Communist-bloc
nations.  As a group, they produce more than 60 percent of the
world's wealth and enjoy the world's highest per capita incomes
(OECD, 1973a; 1974b).  The member nations are presented in Table 1

                  Table 1.  OECD MEMBER COUNTRIES
Australia              Greece              Norway
Austria                Iceland             Portugal
Belgium                Ireland             Spain
Canada                 Italy               Sweden
Denmark                Japan               Switzerland
Finland                Luxembourg          Turkey
France                 Netherlands         United Kingdom
Germany                New Zealand         United States
            Special Status Country:  Yugoslavia

(From OECD, 1973a)

     Because economic development of the member nations is its
organizational focus, OECD contains a number of committees asso-
ciated with the various aspects of economic development and growth
These committees and the OECD organizational structure are
presented in Figure 1.  Recognizing that economic productivity
frequently gives rise to environmental problems, the OECD has
concerned itself with both the quantitative and qualitative
aspects of economic development.  In 1970 it transformed its
Committee for Research Cooperation into the more comprehensive
Environment Committee, which is responsible for:

     1.  investigating the problems of preserving or improving
         man's environment, with particular reference to
         economic and trade implications;

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    DEVELOPMENT
    ASSISTANCE
    COMMITTEE

     TECHNICAL
    COOPERATION
    COMMITTEE

   ^ENVIRONMENT
    COMMITTEE
                                    COMMITTEE FOR
                                    SCIENTIFIC AND
                                    TECHNOLOGICAL
                                       POLICY
  ECONOMIC POLICY
   ECONOMIC AND
   DEVELOPMENT
 REVIEW COMMITTEE
   COMMITTEE FOR
 MONETARY a FOREIGN
 EXCHANGE MATTERS
 COUNCIL
EXECUTIVE
COMMITTEE
1 NTERNATIONAL
SECRETARIAT
(divided mto Directorates cover-
ing all the issues treated by
the Committees)














CENTRE FOR
EDUCATIONAL
RESEARCH AND
INNOVATION

SPECIAL
PROGRAMMES

'Formerly the Committee for Research Cooperation
 ( From OECD, I973o)
  Figure  1.    Organizational  Structure  of OECD.

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     2.   reviewing and confronting actions  taken or
         proposed in member nations in the  field of
         environment, together with their economic
         trade implications;

     3.   proposing solutions  for environmental problems
         that would, as far as possible,  take into
         account all relevant factors including cost
         effectiveness; and

     4.   insuring that the results of environmental
         investigations can be effectively utilized in
         the wider framework of the Organization's work
         on economic policy and social development.

     The Environment Committee is assisted by various delegate
groups  concerned with the development of policy in specific
areas of overall environmental problems.   These delegate groups
are presently concerned with the environmental problems of
water and air pollution, automobile and aircraft noise, traffic
congestion and urban transport and hazardous chemicals (OECD,
1973a;  1974a).  The Environmental Committee and its associated
delegate groups are outlined in Figure 2.

WATER MANAGEMENT SECTOR GROUP

     Concern over the problems of decreased water quality caused
by eutrophication had been expressed by OECD even before the
formation of the Environment Committee.  Eutrophication of vari-
ous degrees of severity had been observed in lakes, flowing
waters and impoundments in most of the world's highly developed
nations for many years  (Vollenweider, 1968).  An ad hoc group of
the OECD Committee for Research Cooperation, chaired by 0. Jaag
(EAWAG, Zurich), recommended that a  study be made of the existing
literature on eutrophication, with particular reference to the
roles of phosphorus and nitrogen in the eutrophication process.
This study, completed by R.A. Vollenweider, resulted in the 1968
report, "Scientific Fundamentals of the Eutrophication of Lakes
and Flowing Waters With Particular Reference to Nitrogen and
Phosphorus as Factors in Eutrophication" (Vollenweider, 1968).
This report noted the lack of "sufficient relevant measurement
data" for producing precise guidelines for eutrophication control.

     In 1967, the Water Management Research Group was formed.  In
May, 1968, this group held a symposium in Skokloster, Uppsala,
Sweden on large lakes and impoundments.  A report of this symposium
was published by OECD in 1970 (OECD, 1970).  The Water Management
Research Group became the Water Management Sector Group (WMSG)
after formation of the Environment Committee in 1970 (OECD, 1975).

     In 1971, after the formation of the Environment Committee,
the WMSG established a  Steering Group on Eutrophication Control.
In 1973 and 1974, this  group produced a  series of reports con-
cerning the effects of  detergents, fertilizers and agricultural
wastes, and phosphorus  and nitrogen  wastewater treatment processes

                                10

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                   OECD COUNCIL
                ENVIRONMENT COMMITTEE —
f"
   SECTOR
   GROUP
   ON AIR
MANAGEMENT
I	
SECTOR GROUP
ON UNINTENDED
 OCCURRENCE
OF CHEMICALS
   IN THE
ENVIRONMENT
I	
     SUB-COMMITTEE OF ECONOMIC  EXPERTS
         (ANALYSIS  8  EVALUATION )
                    JOINT AD HOC
                    POLICIES ISSUES
                      GROUP ON
                   WASTE DISPOSAL
  ( From OECD, I973a )
Figure 2.   Organizational Structure  of
             OECD Environment  Committee.
                         11

-------
on water quality.  It also produced the Report of the Water
Management Sector Group on Eutrophication in 1974.  More sig-
nificant, however, was the 1973 report entitled "Summary Report
of the Agreed Monitoring Projects on Eutrophication of Waters"
(OECD, 1973b).  This report was prepared by a WMSG planning group
on measurement and monitoring.  It is this report which outlines
the working plan for the international cooperative eutrophication
study undertaken by OECD.  The OECD North American Project is
part of this cooperative effort.

OECD INTERNATIONAL COOPERATIVE PROGRAM FOR MONITORING OF INLAND
WATERS

Objectives of Study

     In order to better quantitatively define the eutrophication
process and the factors which cause and control it, upon recommen-
dation of the above-mentioned planning group, the WMSG established
a program among the OECD member nations of measurement and monitor-
ing of inland waters.  This international effort was to coordinate
measurements of
-------
Common Measurement System

     Previous attempts to quantitatively categorize freshwater
bodies in terms of tolerance to nutrient inputs, as manifested in
their biological productivity, nutrient budgets and trophic levels,
have been difficult because of the lack of comparable data for
interrelating water bodies.  Such lack of comparable data has
greatly hindered development of criteria for predicting changes in
water quality resulting from changes in nutrient loadings.

     Consequently a common system of measurements was established
early in the study.  In addition to aiding in the choice of eutrophica-
tion control measures in a water body, the common system will also
permit measurement of the effectiveness of a given control measure and
the response of the water body to changing hydrological conditions.

     The system of measurements recommended was divided into
three categories:  physical, chemical and biological.  These
categories were, in turn, divided into "essential" and "desirable"
measurements.  In addition, guidelines were established for the
range of background data considered necessary for providing ade-
quate geographical, morphometric, hydrological and ecological
descriptions of a given water body.

     The essential parameters were those considered necessary
for establishing an accurate representation of trophic conditions
in a given water body.  These parameters would also allow a com-
parison of eutrophication data between water bodies.  In addition,
they would allow the assessment of the effectiveness of control
measures initiated in an attempt to alleviate eutrophication
problems.

     Those parameters which were appropriate for large capacity
laboratories or certain specialized laboratories were considered
"desirable".  In general, the desirable parameters were used to
supplement the "essential" data  (OECD, 1973b).  A summary of these
essential and desirable parameters is given in Table 2.

     Recommended analytical methods were taken from FWPCA (1969),
APHA et al.  (1971) and Golterman (1971).  Recommendations on
sampling techniques included locations, depths and frequencies of
sampling (OECD, 1973b).

Regional Approach

     Recognizing that geographical, ecological, geological and
morphometric factors are of major importance in the eutrophica-
tion process, the WMSG chose to employ a regional approach.  Con-
sequently the WMSG established four voluntary regional projects,
each embracing a family of specified types of water bodies.

     Eighteen member nations agreed to participate in these
projects.  There were three regionally-based projects and one

                                 13

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   Table  2.  SUMMARY OF ESSENTIAL AND DESIRABLE PARAMETERS
             IN  OECD EUTROPHICATION STUDY
    Category
        Parameters
  Physical
    Essential
    Desirable
  Chemical
    Essential
     Desirable
   Biological
     Essential
     Desirable
Temperature, electrical con-
ductivity, light penetration,
color, total solar radiation.
Turbidity.
pH, dissolved oxygen, phos-
phorus, nitrogen, silica,
alkalinity, acidity, calcium,
magnesium, sodium, potassium,
sulfate, chloride, total iron,
Other trace elements and
other micro-pollutants (e.g.
pesticides), hydrogen sulfide,
Phytoplankton (chlorophyll a)
primary productivity, organic
carbon.
Phytoplankton identification
                          14
(by genera and counting);   C
uptake,  zooplankton identifi-
cation  (by genera and count-
ing) .
(From OECD,  1973b)

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functionally-based project in the overall eutrophication study
(OECD, 1973b).   The regional organization and participating
countries are illustrated in Figure 3.  The coordination centers
were to coordinate the activities within a given project  and to
act as vehicles of exchange of information between the  four
projects.  Each individual project's groups of laboratories,
assisted by its coordination center, was responsible for design-
ing and establishing the necessary measurement procedures and
data evaluation methods (OECD, 1973b).

     Each project had a coordinator who was a senior scientist
from one of the institutions or laboratories involved.   Initially,
the Coordinating Group was established as a link between the
Technical Bureau and the WMSG.  However, it was demonstrated that
the Technical Bureau could adequately perform both the  technical
and managerial roles (OECD, 1975).  The overall assessment and
coordination of the four projects was the responsibility of a
group of nationally nominated delegates from those countries par-
ticipating in the study.  This group was to synthesize  the reports
of the four projects into an optimal eutrophication control strat-
egy and report to the WMSG, in principle once a year.

     The program was planned to run four years, from the beginning
of 1973 to the end of 1976.  An overall analysis of the study is
planned for 1977.  Upon completion of the four-year period of
measurements and study, it is expected that a symposium on the
overall interpretation of the results will be convened  in order
to establish the extent to which nutrient loadings determine the
rate of development of eutrophication (OECD, 1973b; 1975).

     The four regional projects are characterized as follows:

     1.  Nordic Project - Reasonably comparable conditions exist
         in this project.  These include the cool climate zone of
         the Baltic and North Sea areas; lakes resulting from
         retreat of the great Quaternary glaciers; comparable
         ecological conditions and equivalent level of  economic
         development and pollution, and close political, cul-
         tural and scientific links.

     2.  Alpine Project - The Alpine regions are the source
         of many European waters.  The Alpine waters are of
         great social and economic significance because they
         represent a great natural amenity and a source of con-
         siderable tourism.  Their ecology is characterized
         by an abundant variety of species which are vulner-
         able to man's interventions.  The Alpine zones repre-
         sent similar hydrological conditions due to comparable
         geography, geology and ecology.  The Alpine zones
         share certain river basins and commissions.

     3.  Reservoir and Shallow Lakes Project - This project
         includes man-made lakes and reservoirs and other

                                 15

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                ENVIRONMENT COMMITTEE
            WATER MANAGEMENT SECTOR GROUP
  ALPINE PROJECT
      AUSTRIA
      FRANCE
     GERMANY
       ITALY
     SWITZERLAND
    COORDINATION
      CENTER-
      ZURICH,
     SWITZERLAND
                     NORDIC PROJECT
                         DENMARK
                         PIN LAND
                         NORWAY
                         SWEDEN
                       COORDINATION
                         CENTER.
                         HELSINKI,
                          Fl NLAND
    RESERVOIR
     PROJECT
                    COORDINATING
                        GROUP

                   DATA PROCESSING
                     8 ANALYSIS
     AUSTRALIA
      BELGIUM
      GERMANY
      IRELAND
       JAPAN
    NETHERLANDS
       SPAIN
   UNITED KINGDOM
    COORDINATION
      CENTER
      SIEGBURG,
      GERMANY
                     NORTH AMERICAN
                         PROJECT
                          CANADA
                       UNITED STATES
                       COORDINATION
                         CENTER:
                        BURLINGTON,
                          ONTARIO
Figure  3
Organizational
Eutrophication
Outline
Study.
of  OECD
                           16

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comparable water bodies (i.e., shallow lakes,
lagoons and estuarine waters).  All are relatively
shallow and have great economic and social values
(e.g., water supply reserves, water sports, fishing,
navigation, etc.).  Water quality control by
manipulation of hydrological or other factors is
more feasible for these water bodies than for
larger water bodies.

North American Project - In contrast to the other
projects, this project is not restricted to study-
ing specific types of water bodies.  Rather, the
trophic states of the involved water bodies span the
trophic spectrum from oligotrophic to eutrophic (OECD,
1973b).
                        17

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                            SECTION V

                   US OECD EUTROPHICATION STUDY


     The major goal of the North American Project is similar to
that of the other projects; namely, to determine the quantita-
tive relationship between the nutrient loading and the result-
ant trophic state (i.e., degree of fertility) of a given water body,

     Its specific objectives are as follows:

     1.  develop detailed nutrient budgets (phosphorus and
         nitrogen) for a selected group of water bodies;

     2.  assess the physical, chemical and biological char-
         acteristics of these selected water bodies;

     3.  relate the trophic states of the water bodies to
         their nutrient budgets and to their limnological
         and environmental characteristics; and

     4.  synthesize an optimal strategy, based on data from
         all four projects, for controlling eutrophication.

The North Ameridan Project consists of studying thirty-four
water bodies in the United States and a larger number of water
bodies in Canada.  The director of the North American Project
is R. Vollenweider of the Canada Centre for Inland Waters
(CCIW) in Burlington, Ontario, Canada.  The United States
Environmental Protection Agency (US EPA) is the lead organiza-
tion for the US portion of the North American Project.  The US
OECD study directors were N. Jaworski and J. Gakstatter
(US EPA, 1973b).  The 34 water bodies in the US OECD study
are presented in Table 3 and their locations are illustrated
in Figure 4.

     The water bodies in the US OECD study differ considerably
in their limnological characteristics and trophic states.  It
is the responsibility of the principal investigator for each
water body to conduct the necessary measurements and to prepare
the necessary reports for his water body.  Nearly all of the
water bodies selected for the US OECD study have been studied
extensively in the past.  Because of these factors and a lack of
funds, no new sampling programs were initiated in the US OECD
study.  Some of the water bodies were also included in the US
EPA's National Eutrophication Survey (NES), thereby providing a
link between the US OECD studies and the NES studies.

                                 18

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            Table 3.
                      LIST OF WATER BODIES IN OECD NORTH AMERICAN PROJECT
                                     (US PORTION)
Water Body
                           Location
Tropic Status
Principal Investigator
Rlackhawk, Camelot-Sher-   Wisconsin
wood, Cox Hollow, Dutch
Hollow, Redstone, Stewart,
Twin Valley and Virginia

Brownie, Calhoun, Cedar,   Minnesota
Harriet and Isles
                           Mew York


                           New York


                           Minnesota



                           New York


                           N. Carolina.
                           Virginia

                           Wisconsin
Cayuga


Dogfish, Lamb and
Meander
heorge

Kerr Reservoir'


Mendota
Eutrophic



Eutrophic


Eutrophic



Mesotrophic


Oligotrophic
Old gotrophic-
Mesotrophic
Eutrophic-
Mesotrophic
Eutrophic
(Changing)
G. Fred Lee, Center
for Environmental Stu-
dies, Univ. Texas at
Dallas
J. Shapiro, Limnology
Research Center, Univ.
Minnesota
L. Hetling, Dept. Env.
Conscrv., State of New
York

R. Oglesby, Cornell
Univ.

S. Tarapchak, NOAA
Great Lakes Env. Res.
Lab, Ann Arbor, Mich.

M. Clesceri, Rensselaer
Polytechnic Inst.

C. Weiss, Univ. North
Carolina.

G. Fred Lee, Center
for Environmental Stu-
dies, Univ. Texas at
Dallas

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                       Table 3 (continued).
LIST OF WATER BODIES IN OECD NORTH AMERICAN PROJECT
        (US PORTION)
ro
o
Water Body
Michigan
Open waters

Nearshore Waters
Minnetonka
Potomac Estuary
Sal lie
Sammamish
Shagawa
Tahoe
Twin Lakes
Wa]do
Location
Wisconsin,
Michigan,
Illinois £
Indiana


Minnesota
Maryland,
Virginia
Minnesota
Washington
Minnesota
California ,
Nevada
Ohio
Oregon
Trophic Status
Oligotrophic

Mesotrophic
Eutrophic
(Changing)
Ultra-Eutrophic
Eutrophic
Mesotrophic
Eutrophic
Ultra-Oligo-
trophic
Eutrophic
(Changing)
U] tra-Oligo-
trophic
Principal Investigator
G. Fred Lee, Center
for Environmental Stu-
dies, Univ. Texas at
Dallas
and
C. Schelske, Great
Lakes Research Division,
Univ. Michigan
R. Megard, Limnology
Research Center, Univ.
Minnesota
N. Jaworski, US EPA,
Corvallis, Oregon
J. Neel, Univ. North
Dakota
E. Welch, Univ.
Washington
K, Malueg, US EPA,
Corvallis , Oregon
C. Goldman, Univ.
California at Davis
D. Cooke , Kent State
Univ.
C. Powers, US EPA,
Corvallis, Oregon

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       Table 3 (continued).   LIST OF WATER BODIES IN OECD NORTH AMERICAN PROJECT
                                     (US PORT EON)

Wa tor Body
Wash: ngton
Weir
Wingra
Location
Washington
Florida
Wisconsin
Trophic Status
Meos trophic
Meso trophic
Eutrophic
Principal Investigator
W.T. Edmondson , Univ.
Washington
P. Brezonik, Univ.
Florida
G. Fred Lee, Center
                                                                  for Environmental Stu-
                                                                  dies, Univ. Texas at
                                                                  Dallas

Trophic Status Index Study                                        J. Shapiro, Limnology
                                                                  Research Center, Univ.
                                                                  Minnesota

Summarization Report -                                            G. Fred Lee and W.
US OECD Project                                                   Rast, Center for Environ-
                                                                  mental Studies, Univ.
                                                                  Texas at Dallas

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WASHINGTON

SAMMAMISH
                             BROWNIE 1
                             CALHOUN I
                              CEDAR )
                             HARRIET f
                               ISLESJ
               "\
              CAYUGA

           /EAST TWIN
           [WEST TWIN
                         CAMELOT-SHERWOOD |
                            DUTCH HOLLOW V
                                REDSTONE /
                                 VIRGINIAj
                                                             POTOMAC	
                                                             ESTUARY
IMENDOTA
[WINGRA
                               BLACKHAWK
                              COX  HOLLOW \
                              TWIN VALLEY /
                                 STEWART I
         KERR 	'•
       RESERVOIR
   IOO  2OO 300
    MILES
     Figure  4.   Locations  of  US  OECD  Water  Bodies.

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GENERAL CHARACTERISTICS OF US OECD WATER BODIES

     The general characteristics of the water bodies in the
US OECD study are presented in Table 4- , which indicates that
the 34 water bodies of the US OECD study include 24 lakes, nine
impoundments and one estuary.  Thus, 71 percent of the water
bodies in the US OECD study are lakes and 26 percent are im-
poundments.  However, several of these water bodies are divided
into separate arms or regions (e.g., Kerr Reservoir and the
Potomac Estuary).  When these separate regions are considered,
there are 38 US water bodies in the US OECD eutrophication study.
Furthermore, several of the US OECD water bodies have been pre-
viously examined and have subsequently undergone remedial treat-
ment for eutrophication (e.g., Minnetonka, Twin Lakes, Washing-
ton).  Thus, although 38 water bodies are included in the US
OECD study, a total of 47 individual nutrient loading-trophic
response relationships were examined.

    The principal investigators classified 24 of the water
bodies as eutrophic (63 percent), seven as mesotrophic (18 percent)
and seven as oligotrophic (18 percent) at the time of the US OECD
study.  These percentages reflect the investigator-indicated
trophic states at the time of submission of final reports.

     Twenty-eight (74 percent) of the water bodies have mean
depths less than ten meters while ten (26 percent) have mean
depths greater than ten meters.  The mean depths range from 1.7
meters (Lake Virginia) to 313 meters (Lake Tahoe).  The water-
shed areas range from 47 hectares (Brownie Lake) to 1.76 x 10
hectares (Lake Michigan).  Sixteen (42 percent) of the water
bodies have surface areas greater than 1000 hectares.  Twenty-
three (61 percent) of the water bodies have hydraulic residence
times (i.e., water body volume/annual inflow volume) of greater
than one year.  The hydraulic residence times range from 0.08 yr
(Lake Stewart) to 700 yr (Lake Tahoe).  Twenty-four (63 percent)
have mean specific conductances of 200 ymhos/cm (25°C) or greater.

     Of the 24 water bodies with mean specific conductances
greater than 200 ymhos/cm, 21 were classified eutrophic, two
mesotrophic and one oligotrophic.    As expected, the single
estuary studied had the highest mean specific conductance ,
ranging from 200-500 ymhos/cm (25°C) at the fresh water input
to 26,000 ymhos/cm at the saline end of the estuary.

     Of the 13 water bodies with less than 200 ymhos/cm mean
specific conductance, seven were oligotrophic, four mesotrophic,
and two eutrophic.  Ultra-oligotrophic Lake Waldo exhibited the
lowest reading, 3 ymhos/cm (25°C).

     The mean alkalinities ranged from 2 mg/1 as CaCO« (Lake
Waldo) to 248 mg/1 (Canadarago Lake).  The distribution was
relatively even, with 18 (47 percent) having mean alkalinities
greater than 100 mg/1 as CaCOQ.
                             O
                              23

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Table 4.   CHARACTERISTICS OF US OECD WATER BODIES

Water Watershed
WATER BODY Trophic Body Area3 ~
(location) Status" Type0 (xlO m )
LAKE BLACKHAWK E I 36.3
(Wise. )
BROWNIE LAKE E L 0.47
(Minn. )
LAKE CALHOUN E L 7.61
(Minn. )
CAMELOT-SHERWOOD E I 90.6
COMPLEX (Wise. )
CANADARAGO LAKE E L 182
( N . Y . )
CAYUGA LAKE M L 2030
(N.Y. )
CEDAR LAKE E L ] . 63
(Minn. )
COX HOLLOW LAKE E I 16.1
(Wise.)
DOGFISH LAKE ° I, 0.88
(Minn. )
DUTCH HOLLOW E I 12.b
LAKE (Wise. )
Water
Body
Surface
Area r ?
(xlO m
8. 90

0.73

17.0

28.3

75. 9

1720

6. 90

3. 88

2.91

8.50

Mean
Hydraulic
Mean Residence
Depth6 Timef
) (m) (yr)
4.9 0.5

6.8 -2.0

10.6 3.6

3.0 0.09-0.14

7.7 0.6

54.5 8.6

6.1 3.3

3.8 0 .5-0 .7

4.0 3.5

3.0 1.8

Mean Mean Con-
Secchi ductivity
Depth (pmhos/cm
(m) @ 25°C)
3-6 471

1.5 400-475

2.1 400-500

2.0 311

1.8 223

2.3 575

1. 8 400

1 .5 440

2.5-2.7 16-17

0.8 252

Mean Alka-
linity
(mg/1
as CaC03)
227

123-136

80-123

125

248

102

71-109

205

8-10

133


-------
                 TnMe 'I (rcm I i lined ) .   CHARACTERISTICS OF US  OECD WATER  BODIES'1
"
WATLR BODY Trophicb
(Location) Status
LAKE GEORGE 0-M
LAKE HARRIET E
(Minn. )
LAKE OE THE E
ISLES (Minn. )
to
en KERR RESERVOIR E-M
(N. Carol ina-Vir . )
Roanoke Arm
Nutbush Arm
LAMB LAKE 0
(Minn. )
MEANDER LAKE 0
(Minn. )
LAKE MENDOTA E
(Wise. )
LAKE MICHIGAN 0-M
Wa tcr
Body
Water Watershed Surface
Body Area$ „ Area^ „
Type0 (xlO rn ) (xlO ni )
I, 606 11 UO
L 't. 80 14.3

L 2.85 4.20


I 20,200 1754

1250
504
L 1.96 3.97

L 1.69 3.60

L 686 39U

L 176,000 580,000
Mean
Depth6
(m)
18.0
8. 8

?. 7


-

10. 3
8.2
4 .0

5.0

12.0

8U
Mean
Hydraulic Mean Mean Con-
Residence Secchi ductivity
Timef Depth (vimhos/cm
(yr) (m) @ 25 C)
8.0 8.5 86
2.4 2.4 360-425

0.6 ].0 380-470


_ _

0.2 1.4 100
5.1 1.2 123
2.3 1.8-2.2 U7

2.7 3.0-3.1 17-20

4.5 3.0 300

30-100h
Mean Alka-
linity
(mg/1
as CaCO,)
21
92-124

68-131


-

28
22
30-36

8

160

-
(Wise., Mich.,
111. ,  Ind. )

-------
Table 4 (continued).   CHARACTERISTICS OF US OECD WATER BODIES0
WATER BODY Trophic.
(Location) Status
LAKE MICHIGAN
(cont'd)
Nearshore
Waters
Offshore
Waters
Open Lake
Waters
LAKE MINNETONKA


M

M
0

(Minn.)
Pre-sewage E
Treatment (1969)
Post-sewage E-*M
Treatment (1973)
POTOMAC ESTUARY
(Maryland, Vir. )
Upper Reach
Middle Reach
Lower Reach
LAKE REDSTONE
(Wise. )
U-E
E

Water
Body
Water Watershed Surface
Body AreaSJ , Area, „
Typec (x!0b rn ) (xlO m


_

_
_
L 371g 262
L 371g 262
L 371g 262
E 38,000 9644
574
2120
6950
I 76.7 25.2

Mean
Hydraulic Mean
Mean Residence Secchi
Depth6 Timef Depth
) (m) (yr) (m)


_

-
_
8.3
8. 3
8.3
4.8
5.1
7.2
4. 3



2.3

7.0
_
6.3g
1.5
1.8
0.04 0.4-0.8
0.18 0.5-1.3
0.85 1.0-2.3
0.7-1.0 1.6

Mean Con- Mean Alka-
ductivity linity
(vmhos/cm (mg/1
@ 25°C) as CaC03)


265

260
255
^
317
200-500
600-17,000
17,000-26,000
260



107

106
113
,_
250
250
70-110
60- 85
65- 85
125


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               Table  4  (continued).  CHARACTERISTICS  OF  US  OECD WATER BODIES0
WATER BODY Trophic,
(Location) Status
LAKE SALLIE
(Minn. )
LAKE SAMMAMISH
(Wash. )
SHAGAWA LAKE
(Minn. )
LAKE STEWART
(Wise. )
E

M

E

E

LAKE TAHOE U-0
(Calif. ,Nev. )
TWIN LAKES
(Ohio)
EAST TWIN LAKE
Pre-sewage
Treatment(1972
Post-sewage
Treatment(1974
WEST TWIN LAKE
Pre-sewage

_

_
E
)
E
)
_
E
Water Mean
Body Hydraulic
Water Watershed Surface Mean Residence
Body Aread Area,. Depth6 Timef
Type0 (xlO m ) (xlO ni ) (m) (yr)
L 1540

L 273

L 269

I 2.07

L 1310

L 3. 34

L
L

L

L
L
53.0

198

92. 0

0.25

4990

_

2.G9
2.69

2 .69

3.40
3.40
6.4 1.1-1.8

17.7 1.8

5.7 0.8

1.9 0.08

313 700

_ _

5.0
5.0 0.80

5.0 0.50

4.3
4.3 1.6
Mean Mean Con- Mean Alka-
Secchi ductivity linity
Depth (ymhos/cm (mg/1
(m) @ 25 C) as CaCOj)
280-360 162

3.3 94 33

2.3 60 22

1.4 540 213

28 92 43

- — -

_ _ _
1.6 374

1.9 366 105

— — _
2.2 411
Treatment(1972)
Post-sewage
E
L
3.40
4.3 1.0
2.3 380 106
Treatment(1974)

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                   Table 4 (continued).  CHARACTERISTICS OF US OECD WATER BODIES3


WATER BODY
(Location)

Trophic.
Status
TWIN VALLEY LAKE E
(Wise. )
LAKE VIRGINIA
(Wise. )
WALDO LAKE
ro (Ore.)
00

E

U-0


LAKE WASHINGTON
(Wash. )
Pre- sewage
Diversion
Post-sewage
Diversion
LAKE WEIR
(Fla. )
LAKE WINGRA
(Wise. )

E
(1964)
M
(1974)
M

E

Water
Body
Type
I

I

L


L

L

L

L

L

Water
Body
Watershed Surface
Aread
(xlO6 m2
31.1

6.48

79


1590

1590

1590

46.0

14.0

Area,
) (x!0b in ]
6.07

1.82

270


876

876

876

240

13.7

Mean
Hydraulic Mean Mean Con-
Mean Residence Secchi ductivity
Depth Timef Depth (pmhos/cm
I (m) (yr) (m) @ 25°C)
3.8

1.7

36


33

33

33

6.3

2.4

0.4-0.5 1.5 370

0.9-2.8 1.2 230

21 28 3


2.4

2.4 1.2 80

2.4 3.8 81

4.2 1.9 133

0.4 1.3

Mean Alka-
linity
(mg/1
as CaCOg)
175

64

2


-

2S

45

12

153

aAs reported by \JS OECD investigators.  See Summary Sheets (Appendix II)

 Investigator-indicated trophic status:  (U-E) = Ultra-Eutrophic
                                         (E)   = Eutrophic
                                         (M)   = Mesotrophic
                                         (0)   = Oligotrophic
                                         (U-0) = Ultra-Oligotrophic

-------
                     Table '4 (continued).  CHARACTERISTICS OF US OECD WATER BODIES3
EXPLANATION:  (continued)
 Water body type :   E = Estuary
                   I = Impoundment
                   L = Lake

 Includes lake surface area
e                                 32
 Mean depth = water body volume (m ) /water body surface area (m )

 Hydraulic residence time = water body volume (m )/annual inflow volume (m /yr)
a
 Values for whole  lake.  All other data is only for Lower Lake Minnetonka

 Range of values as reported in the literature; most accurate range is assumed  to
     be 70-100 years.  See Piwoni et al. (1976) for discussion of Lake Michigan
     hydraulic residence time.

Dash (-) indicates data not available.

-------
     Twenty-eight (74 percent) had mean Secchi depths less than
three meters.   No Secchi data were available for two water bod-
ies.  Of the 28 water bodies with Secchi depths less than three
meters, 22 were classified by their respective investigators as
eutrophic, five mesotrophic and one oligotrophic (Dogfish Lake).
Within the eight water bodies of three meters or greater Secchi
depths, five were classified oligotrophic,  one mesotrophic and
two eutrophic (Lakes Blackhawk and Mendota).  The mean Secchi
depths ranged from 0.6 meters in the Upper Reach of the Potomac
Estuary to greater than 28 meters (Lakes Tahoe and Waldo).

DATA  REPORTING METHODOLOGY

      The  general approach involved  in the US  OECD study  is pre-
sented in the Final  Report  Outline  (Appendix  I).  This Final
Report Outline was prepared by the  North American Project parti-
cipants and served both as  a  guide  to the types of information
and studies needed in the North American Project and as  an out-
line  for the presentation of  the data generated in the North
American  Project in  standardized Final  Reports.  Part of the in-
formation in the Final Report Outline was suggested by the WMSG
as necessary "background data" (OECD, 1973b).

      The  Final Report Outline begins with a short introductory
section,  followed by a brief  geographical description of the
water body.  This includes  its latitude, longitude and altitude,
the watershed area,  general climate data, general geological
description, vegetation, watershed  population,  land usage and
wastewater discharges into  the water body.  Next is a. brief
morphometric and hydrologic description of  the  water body, in-
cluding its surface  area, volume, mean  and  maximum depths,
ratio of  epilimnion  to hypolimnion, duration  of stratification,
lake  sediment'.types , seasonal precipitation variation, water
budget, water currents"and  hydraulic residence  time.  This is
followed  by a limnological  characterization of  the water body,
including a physical, chemical and  biological summary.   A
nutrient  budget  summary,  including  phosphorus and nitrogen
inputs, follows  the  limnological characterization.  Finally,
there is  a discussion section which includes  a  delineation of
water body trophic status and discussion of the general  lim-
nological characteristics.  In addition, the  degree of correla-
tion  between the water body nutrient loading  and trophic re-
sponse is discussed  in detail.  These two parameters are also
to be discussed  in relation to the  water body's general  lim-
nological characteristics.

      The  US  OECD study  "Summary  Sheets" (Appendix  II) were  de-
vised to  summarize  the  important  loading  and  response parameters
of the US OECD water bodies.   They  include  the water body name
and type, watershed  and water body  surface  area, mean depth,
water residence  time,  important  trophic response parameters
 (e.g., nutrient  and  chlorophyll  a  concentrations,  primary


                                30

-------
productivity) and nutrient loadings.   The Summary Sheets and the
Final Report Outline were prepared to allow the presentation of
data in a standardized form.


US OECD EUTROPHICATION STUDY AND OTHER US EUTROPHICATION
CONTROL PROGRAMS

National Eutrophication Survey

     Several years ago, the US EPA (1975a) initiated the National
Eutrophication Survey.  This Survey was designed to study approx-
imately 800 water bodies throughout the US for which estimated
nutrient load-response relationships would be ascertained.
Because of funding limitations, sampling of tributaries and water
bodies was limited to one year and was not intensive.  The US
OECD eutrophication study provides similar information for a
smaller number of water bodies and was generally based on a much
more intense sampling program.  For the water bodies common to both
programs, a comparison of the two approaches will aid the US EPA
and other water pollution regulatory agencies in assessing the
validity of the results and conclusions from the National Eutro-
phication Survey.

Public Law 92-500

     Section 314-A of Public Law 92-500 requires all the states
in the US to classify their publicly-owned water bodies as to
trophic status.  It further requires the states to initiate
eutrophication control measures in water bodies deemed excessively
fertile.  Thus, the overall aims of the US OECD eutrophication
study, the US EPA's NES study and the intent of Public Law
92-500, Section 314-A, are generally identical.  They are to
ascertain what trophic classification or index system should be
used, what parameters should be measured, how a given set of
conditions in a water body can be related to its trophic status,
how one predicts response of a water body to a change in a
chemical, biological or physical parameter and what the aquatic
plant trophic response will be to a given water body's nutrient
input.  By attempting to answer questions of this type, the US
OECD eutrophication study can be used by the states to help them
fulfill the mandate of Section 314-A of Public Law 92-500.

     Public Law 92-500 also requires the US EPA to develop water
quality criteria.  In October, 1973 the US EPA released draft
proposed criteria for public comment (US EPA, 1973c).  In
November, 1975 the US EPA released revised draft Quality Criteria
for Water (US EPA, 1975b) and again asked for comment.  While
no criteria were proposed for phosphorus as an aquatic plant
nutrient, the US EPA suggested in the November 1975 criteria that
a nutrient loading-response relationship similar to those being
investigated in the US OECD eutrophication study be adopted.


                               31

-------
USE OF N:P RATIOS IN DETERMINING THE AQUATIC PLANT GROWTH
LIMITING NUTRIENT IN NATURAL WATERS

     The role of phosphorus and nitrogen as aquatic plant (i.e.,
algae and macrophytes) nutrients in the primary productivity and,
hence , in the eutrophication of natural waters has been well-
documented (Sawyer, 1947; American Water Works Association, 1966;
Vollenweider, 1968; Edmondson, 1970b; Lee,  1971;  Ryther and Dunstan.
1971; Maloney et_ al_. , 1972; Powers et al. ,  1972; Martin and Goff,
1972; Shannon and Brezonik, 1972; Brezonik, 1973; Lee, 1973;
Vallentyne, 1974; United States Environmental Protection Agency,
1974a; Schindler and Fee, 1974; Vollenweider, 1975a; and Jones
and Bachmann, 1975, to cite but a few).  The effects of man-
induced nutrient inputs , as opposed to natural nutrient inputs,
in accelerating the eutrophication process has also been studied
in detail (Sawyer, 1952; Curry and Wilson,  1955; Shapiro and
Ribeiro, 1965; Maloney, 1966; Vollenweider, 1968; Bartsch, 1970;
Stumm and Morgan, 1970; Bartsch, 1972; Edmondson, 1972; Beeton and
Edmondson, 1972; and Vallentyne, 1974).  Various other elements
or compounds have been suggested as affecting or limiting the
eutrophication process, including iron, molybdenum, nitrate and
sulfate, vitamins and other organic growth factors, carbon and
silicon (Goldman, 1960; Menzel and Ryther,  1961; Goldman and
Wetzel, 1963; Goldman, 1964; Lange, 1967; Kuentzel, 1969; Pro-
vasoli, 1969; Kerr et_ al_. , 1970; Schelske and Stoermer, 1972).
However, most of these effects are either site-specific, or else
are temporal in nature and do not persist over the annual cycle.
Today, it is generally accepted that the phosphorus and nitrogen
in a water body, rather than the above-mentioned compounds, control
or limit the eutrophication process through their roles as aquatic
plant nutrients in the primary productivity of the water body.
However, not only are the absolute quantities of phosphorus and
nitrogen in a water body of importance in the eutrophication
process, but also their relative quantities seem to be a key
factor in determining which of these two elements will limit the
overall process.

The Limiting Nutrient Concept

     A nutrient will be consumed or assimilated by an organism in
proportion to the organism's need for that  nutrient.  However,
it was noted as early as 1840 by Justus Liebig that growth of a
crop was not generally limited by the nutrients needed in large
quantities, which were often abundant in the environment, but
rather by the nutrients needed in minute quantities , which were
often scarce.  This observation forms the basis of one of the
oldest laws of plant nutrition, LiebigTs "Law of the Minimum"
(Odum, 1971).  Simply stated, Liebig's law states'that growth
of an organism is limited by the substance  or foodstuff which
is available to it in the minimal quantity  relative to its needs
for growth or reproduction.  This principle has also been applied
to factors other than nutrients, including light and temperature.

                                32

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However, for the purposes of this discussion, the limiting nutrient
concept, as Liebig's Law of the Minimum has come to be called, will
be restricted to aquatic plant nutrients.

Nitrogen and Phosphorus as Limiting Nutrients

     The nutrients (i.e., elements or compounds) needed in relative-
ly large quantities by aquatic plants include carbon, hydrogen,
oxygen, sulfur, potassium, calcium, magnesium, nitrogen and phos-
phorus (Fruh, 1967).  In addition, there is a requirement for
traces of micronutrients as listed in Table  5.

         TABLE 5.  SUMMARY OF AQUATIC PLANT MICRONUTRIENT
                         REQUIREMENTS


   Process                       Trace Element Required


Photosynthesis                   Manganese, iron, chloride,
                                 zinc and vanadium

Nitrogen Fixation                Iron, boron, molybdenum and
                                 cobalt

Other Functions                  Manganese, boron, cobalt,
                                 copper and silicon


(After Shannon, 1965, as cited in Fruh, 1967)

     Among these macro- and micronutrient requirements, nitrogen
and phosphorus are generally considered to be the aquatic plant
nutrients of major importance in the eutrophication process.

     Recently, the possible role of carbon as a limiting nutrient
has been proposed (Lange, 1967', Kuentzel, 1969; Kerr et al. , 1970).
However, the work underlying the so-called "Lange-Kuentzel-Kerr
thesis" has been questioned on several grounds (Shapiro, 1970;
Schindler, 1971; 1977; Fuhs ejt a 1. , 1972; Goldman et_ al. , 1972).
Goldman et: al. (1972) have reported that the results of Kerr ejt al.
(1970), indicating C02 to be the limiting nutrient in their
experiments, were due primarily to faulty experimental design.
The conclusions of Kerr et_ al. (1970) were supported mainly by
laboratory data with samples which contained surplus phosphorus
and a limited C02 content.  Consequently, carbon was limiting
almost from the beginning of their experiments.  A similar situa-
tion is frequently seen in wastewater stabilization ponds where,
because of the excessive quantities of phosphorus and nitrogen
relative to carbon, total algal productivity is known to be
limited by carbon (Goldman e_t al. , 1972).  Such a situation
generally does not appear to occur in natural waters.  Maloney
et al. (1972), in laboratory assays on water from nine Oregon

                                33

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lakes, and Powers et aj^. (1972), in field experiments on lakes in
Oregon and Minnesota, demonstrated that carbon addition to the
waters had no effect on algal growth rates.   Further, there
appeared to be no correlation between algal rates and carbon con-
centration in the water bodies.  Schindler (1977) reported that
the bottle bioassay experiments used to test the carbon limita-
tion theory were faulty in that they eliminated the turbulence
of water and its interaction with the overlying atmosphere  and
because no attempt was made in the experiments to simulate the
proportion of alkalinity supplied by hydroxyl  ions  in natural
waters which affects the rate at which carbon is taken into the
aquatic ecosystem.

     Shapiro (1973) has demonstrated that a shift from blue-green
algae to green algae resulted when C02 was added to their water.
Presumably, a shift from green algae to blue-green algae would
occur in natural waters as the CC>2 content of the water was
depleted.  Shapiro concluded that this shift to blue-green algae
would likely occur because they appear to be more efficient in
utilizing C02 in waters of low C02 content.  This shift in algal
types, rather than a general reduction in algal biomass, implies
that the total algal content remains relatively unaffected in
waters low in COy-  Rather, there is a shift to blue-green algal
types because of their nutrient uptake kinetics in low CC>2 waters.
Thus, a low CC>2 content in natural waters will not necessarily
limit algal growth, but rather can shift the dominant algal types
from green to blue-green algae without significantly affecting
the overall primary productivity and algal biomass.

     Recently James and Lee  (1974) have shown similar results in
examination of inorganic carbon limitation in natural waters.
According to their model, inorganic carbon limitation could con-
ceivably occur inflow alkalinity waters.  However, they also
indicate that the types, rather than quantities, of algae present
in a water body could be significantly affected by the amounts
and forms of inorganic carbon present.  Under such conditions,
there may be no noticeable change in total algal biomass, even
though the inorganic carbon content of the water may drop to
apparently growth-limiting levels.

     As a result of these above-mentioned studies, it is generally
accepted today among investigators that carbon will not usually
be a limiting nutrient in natural waters, except under certain
well-defined conditions.  These special conditions would include
sewage lagoons, already eutrophic water bodies, laboratory flasks
with artificial media or special situations affecting the amounts
of available inorganic carbon,  such as very low alkalinity lakes
or extremely hard water bodies  (Goldman et al., 1972; James and
Lee, 1974).  As such conditions occur infrequently in nature,
carbon limitation of total algal growth would be rare in most
natural waters.

     In addition to the many works reported on the role of nitrogen
and phosphorus in the eutrophication of natural waters  (Sawyer,

                                34

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1947; Hutchinson, 1957; Vollenweider,  1968; Lee, 1971; Vallentyne,
1974; Vollenweider and Dillon, 1974),  it has also been observed
that these two nutrients are usually present only in small quan-
tities in natural waters during periods of excessive algal growths
(Mackenthun e_t aJL. ,  1964, as cited in Fruh, 1967).  Vallentyne
(1974) has indicated the special significance of nitrogen and
phosphorus among the 15 to 20 elements commonly needed for the
growth of aquatic plants by calculating the demand:supply ratios
of these essential elements.  According to Vallentyne (1974),
aquatic plants have a certain demand for nutrients,  for their growth
and reproduction, in proportion to the quantities of the nutrients
in their cells.  When one or more of these nutrients is present
in short supply relative to the others, then the overall primary
productivity of the aquatic plant population will be limited by
the rates of supply of these nutrients.  Thus, a "demand:supply"
ratio can reveal the nutrient most likely to limit productivity.
The higher this demand:supply ratio, the more a particular nutrient
will limit growth.  The demand:supply ratios, based on a "world
average", were calculated by determination of the chemical composi-
tion of an average aquatic plant community and dividing this
composition by the mean chemical composition of the river waters
of the world.  These demand:supply ratios are presented in Table 6.
The  dominant role of phosphorus and nitrogen is clearly illus-
trated in Table 6 by their very high demand: supply ratios,
relative to all the other elements normally needed by aquatic
plants.  This is especially prominent during the midsummer  (i.e.,
during the growing season).

           TABLE 6.   DEMAND:SUPPLY RATIOS FOR THE MAJOR
                       AQUATIC PLANT NUTRIENTS
                                   Demand:Supply
Element                    Late Winter         Midsummer
Phosphorus                 80,000              up to 800,000

Nitrogen                   30,000              up to 300,000

Carbon                      5,000              up to 6,000

Iron, Silicon              Variable, but generally low

All other elements                   < 1,000


 Prior to spring bloom

 At algal maximum growth period


(Taken from Vallentyne, 1974)

                                35

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     Thus, nitrogen and phosphorus are the two elements most often
found to be limiting aquatic plant growths.  There have been a
few instances in which other elements have been found to have a
cause-effect role in limiting growth, including silicon (Schelske
and Stoermer, 1972) and iron (Welch et al . , 1975).  However, the
overall importance of these exceptions is not comparable to the
dominant roles played by phosphorus and nitrogen in the eutrophica-
tion process.

Interaction Between Biotic and Abiotic Factors in Determining
Limiting Nutrients and Algae Nutrient Stoichiometry

     It is a long-recognized principle in ecology that inter-
actions between organisms and their environment are reciprocal
(Redfield, 1958; Odum, 1971).  The environment determines the
conditions under which an organism lives.  Organisms respond to
changes in their physical environment by altering their metabolism
or growth requirements.  Algae can directly influence their environ-
ment by changing the concentration of nutrients and other sub-
stances in the water by metabolic uptake, transformation, storage
and release.  This is usually related to reciprocal changes in
algal biomass.  This exchange between algal biomass and nutrient
concentration in natural waters is a cyclic process, which must
always be considered in any attempt to understand the chemistry
in aquatic environments (Redfield et al . , 1963; Stumm and Morgan,
1970).

     This cyclical exchange is a two-phase process, including
synthesis and regeneration.  With algae,  the synthesis phase
consists of withdrawal of nutrients, especially nitrogen and
phosphorus, from the water during photosynthesis.  These nutrients
are withdrawn from the water in the proportions required for
growth of the algae.  The regeneration phase occurs when the
elements are returned to the water as decomposition products and
excretions of the algae, the higher trophic level species which
feed upon them and the microorganisms which decompose their
organic debris (Redfield et al . , 1963).

     The proportions in which algal nutrients in natural waters
enter into the cyclical process described above is determined by
the elementary composition of the algal biomass.  It is generally
accepted that algae need a relatively fixed atomic ratio of
carbon to nitrogen to phosphorus of 106 to 16 to 1 (i.e.,
(106C:16N:1P) (Redfield, 1958; Redfield et_ al . , 1963; Vollen-
weider, 1968; Ketchum, 1969; Lee, 1973).   ThTs observation has
a basis in the simple Stoichiometry of the photosynthesis-
respiration reaction as it occurs in nature, as illustrated in
the following equation:

106 C02 + 16 N0~ + HPO* + 122 H20 + 18 H+ + trace elements
                              {c   H   0   *  p } + 138 0
            < respiration ---   106 263 110 16 l
                              algal protoplasm

(Taken from Stumm and Morgan, 1970)

                                  36

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     The 106C:16N:1P atomic ratio was obtained from the early
work of Redfield (1934) and Fleming (1940), as cited in Redfield
et_ al. (1963), who examined the organic matter in plankton samples
obtained in sea water for the relative quantities of the principal
elements present in the plankton.  The C:N:P atomic ratio values
represent an average of the carbon, nitrogen and phosphorus con-
tent present in phytoplankton and zooplankton, as illustrated in
Table 7.

            TABLE 7.  ATOMIC RATIOS OF C, N AND P PRESENT
                            IN PLANKTON
                                 C          N          P


Zooplankton                      103        16.5       1

Phytoplankton                    108        15.5       1

Average Value                    106        16         1


(Taken from Redfield et al., 1963)


     In this discussion, attention is centered on nitrogen and
phosphorus since it is the relative quantities of these two
elements, rather than carbon, that is likely to limit or control
algal growth, and thereby the eutrophication process, presuming
all other physical and chemical factors are optimal for algal
growth.

     The N:P ratios listed above may change as a function of the
aquatic environment.  Harris and Riley (1956, as cited in
Redfield et al., 1963), studying plankton from Long Island Sound,
reported that while the average N:P atomic ratio in phytoplankton
in their study was 16:1, the average zooplankton N:P ratio was
24:1.  Further, differences during the annual cycle varied as
much as 25 percent, with zooplankton having the highest N:P
ratios in winter and spring.  Ketchum and Redfield (1949, as
cited In Redfield et al., 1963), using mass cultures of the
freshwater algae Chlorella pyrenoidosa, demonstrated that a wide
variation in the N:P~ ratio can occur under extremes of nitrogen
and phosphorus concentrations in the growth medium.  In their
experiments, normal algal culture cells contained an N:P ratio of
about 6:1.  By contrast, phosphorus deficient cells exhibited an
N:P ratio as high as 31:1, while nitrogen deficient cells would
show an N:P ratio of 3:1 or less.

     Fuhs et al. (1972), using Cyc_lotella nana in laboratory
cultures, have shown that under severe phosphorus limitation,
the N:P ratio can rise to 35:1.  It can drop to very low levels

                                 37

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when nitrogen is limiting as a result of "luxury consumption" of
phosphorus.  Fitzgerald (1969) has also demonstrated, with the
use of enzymatic and tissue assay procedures, that the N:P ratio
in algae and aquatic weeds can vary widely, depending on whether
nitrogen or phosphorus is present in excess in the growth medium.

     However, while laboratory studies have demonstrated a
marked variation in algal N:P ratios because of the relative
quantities of nitrogen and phosphorus in the growth medium, field
studies have shown that rarely do such variations occur in natural
waters.  Generally, neither phosphorus nor nitrogen are present
in natural waters in excessive quantities relative to the other.
Consequently, algae in natural waters do not usually contain
nitrogen and phosphorus in the ratios induced by the artificial
conditions of severe phosphorus or nitrogen limitation in the
laboratory studies.  This is illustrated in examination of the
nitrogen and phosphorus content of algae from natural waters in
the southeastern US (Table 8).

           TABLE  8.  CHEMICAL COMPOSITION OF SOME ALGAE
           FROM PONDS AND LAKES IN THE SOUTHEASTERN US
  Algae                               N:P Atomic Ratio
Chara
Pithophora
Spirogyra
Giant Spirogyra
Rhizoclonium
Oedogonium
Mougeotis
Anabaena
Cladophora
Euglena
Hydrodictyon
Microcystis
Lyngbya
Nitella
22:1
20:1
33 :1
22 :1
18:1
73:1
16:1
27:1
9 :1
27:1
36:1
27:1
36 :1
27 :1
Amphizomenon                                 16:1
 (Based  on  Federal Water  Pollution  Control Administration,  1968,  as
 cited in Goldman et  al.,  1972)
                                 38

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     Examination of Table 8 shows, with few exceptions, that in
general the N:P ratio of the algae varies between 16:1 to 27:1.
This ratio is smaller than the 35:1 ratio shown with Cyclotella
nana under severe phosphorus limitation in laboratory cultures
(Fuhs et al., 1972) and higher than that shown with Chlorella
pyrenoidosa under severe nitrogen limitation (Ketchum and
Redfield, 1949, as cited in Redfield et al., 1963).  If the
minimum and maximum values are omitted, the mean N:P atomic ratio
of the algae is 24:1 (standard deviation = 8).   Even if all
values are included, the mean N:P atomic ratio in Table 8 is
27:1 (standard deviation = 15).  Thus, generally, algal popula-
tions in natural waters do not exhibit the extremes in cellular
N:P ratios seen in algal laboratory cultures.

     Thus, even in spite of some variation, it is generally
accepted that the N:P atomic ratio in natural algal populations
remains constant enough to be used in making reasonable pre-
dictions as to which of these two elements is likely to limit
algal growths in natural waters.

The Limiting Nutrient Concept As Applied In The US OECD
Eutrophication Study

     Presumably, as a result of the photosynthesis reaction,
algae will assimilate nitrogen and phosphorus from their aquatic
environment in a stoichiometric atomic ratio of approximately
16N:1P until one of these two nutrients becomes depleted in
the water body.  At that time, the nutrient present in the water
body in the lowest concentration, relative to the stoichiometric
needs of the algae, will limit subsequent growth of the algae.
An examination of the water body at that time for its content of
nitrogen and phosphorus would indicate which of these nutrients
had been depleted by the algae (i.e., which nutrient was the
limiting nutrient).  If the N:P atomic ratio in the water body
fell below 16, this would mean there were less than 16 nitrogen
atoms per each phosphorus atom in the water.  Since this is
below the 16N:1P stoichiometric needs of the algae, the algal
biomass in the water body at that time would be controlled or
limited by the quantity of nitrogen present in the water body.
The amount of phosphorus present in the water body at that time
would have no influence, in terms of limiting algal growth, since
it would be present in excess quantities relative to the stoi-
chiometric requirements of the algae.  The opposite would be true
if the N:P atomic ratio were greater than 16.  Thus, an examin-
ation of the relative quantities of nitrogen and phosphorus in
a water body at a given time, especially during the growing season,
will indicate which of the two nutrients is "left over" after
the other has been depleted by the algae.  Clearly, the nutrient
which is present in large quantities (i.e., left over) during
periods of excessive algal growths is not limiting growth of the
algae.  Rather, the depleted nutrient is the one which would be
controlling or limiting the algal growth.  Other algal metabolic
processes may also be occurring at the same time, such as luxury

                                 39

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consumption of phosphorus in nitrogen-limited waters (Fitzgerald,
1969; Lee, 1973), but in general growth will be controlled by the
nutrient in the water body which has been depleted, relative to
the stoichiometric requirements of the algae.

     Attention must be given to the forms of the nutrients avail-
able for algal and macrophyte growth, rather than to the total
nitrogen or phosphorus content of the water body.  Cowen and Lee
(1976a) demonstrated that up to 30 percent of the particulate
phosphorus in urban runoff can be converted to algal-available
phosphorus (i.e., soluble orthophosphate) in about 20 days.  In
addition, Cowen et al., (1976a) showed that up to 70 percent of
the organic nitrogen in urban runoff can be converted to in-
organic forms (i.e., NHn+NOo+NO^ as  N) available for algal growth
in 35 to 50 days.  Similar findings were shown with river waters
tributary to Lake Ontario (Cowen et al., 1976b).  However, since
algal blooms are rapidly-occurring short-term events, it is the
quantity of the algal-available forms of nitrogen and phosphorus
present at any given time in a water body, rather than the
organic fraction, or the quantities of the total phosphorus or
nitrogen, that will determine which will be Limiting algal growths.
The available form of phosphorus in natural waters consists of
the soluble orthophosphate fraction.  The available nitrogen
forms consist of ammonia, nitrate and nitrite.

     The limiting nutrient concept, as illustrated in the N:P
ratio, has been applied to the US OECD water bodies.  A summary
of the limiting nutrients in the US OECD water bodies, as
indicated by their respective principal investigators, is pre-
sented in Table 9.  In addition, the US OECD water bodies were
examined for their content of available nitrogen and phosphorus
and the mass ratios of inorganic nitrogen:soluble orthophosphate
(as N:P) were determined.  The mass ratios of N:P, rather than
the atomic ratios, were computed because of ease of directly using
the inorganic nitrogen and soluble orthophosphate concentrations
reported by the US OECD investigators.  Since the concentration
volumes were the same,  the inorganic nitrogen:soluble ortho-
phosphate mass ratio was the quotient of the inorganic nitrogen
concentration over the soluble orthophosphate phosphorus con-
centration.  Incorporating the atomic weights of nitrogen and
phosphorus, an N:P atomic ratio of 16:1 corresponds to an N:P
mass ratio of 7.2:1.  Using Selenastrum algal assays, Chiaudani
and Viglis (1974) have shown that at N:P mass rat-ios below 5:1,
nitrogen was limiting, while at N:P ratios of 10:1 or greater
phosphorus was limiting.  Between N:P mass ratios of 5-10 either
could be limiting algal growth.  In this discussion, the critical
N:P mass ratio was taken as 7-8:1.  A similar N:P ratio was also
used by Schindler (1977) to define the limiting nutrient in his
whole-lake studies in the Canadian Experimental Lakes Area.  The
N:P mass ratios of the US OECD water bodies are presented in
Table 10.


                                40

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       TABLE 9.  SUMMARY OF LIMITING AQUATIC PLANT NUTRIENTS
                    IN US OECD WATER BODIES
Water Body
Limiting Aquatic
Plant Nutrient3-
Blackhawk (E)u
Brownie (E)
Calhoun (E)
Camelot-Sherwood Complex (E)
Canadarago (E)
Cayuga (M)
Cedar (E)
Cox Hollow (E)
Dogfish (0)
Dutch Hollow  (E)
George (0-M)
Harriet (E)
Isles (E)
Kerr Reservoir (E-M)

Lamb (0)
Meander (0)
Mendota (E)
Michigan (0-M)
Lower Lake Minnetonka (E+M)
Potomac Estuary (U-E)
Redstone (E)
Sallie (E)
     P
     P
     P (summer)'
     P
     N-upper ends of both arms;
       shifting to P-limitation as one
       moves to lower ends of both arms
     P-open waters;
       most nearshore waters
     N-some nearshore waters
       with restricted circula-
       tion
     P (summer)
     N-in upper £ middle
       portions (summer)
     P-in lower portion,
       and in upper and middle
       portions rest of year
                                      ("P appears not to be
                                      limiting above a certain
                                      level")

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     TABLE 9.  (continued)  SUMMARY OF LIMITING AQUATIC PLANT
                   NUTRIENTS IN US OECD WATER BODIES
Water Body
Limiting Aquatic
Plant Nutrient3
Sammamish (M)
Shagawa (E)
Stewart (E)
Tahoe (U-0)
Twin Lakes (E)
Twin Valley (E)
Virginia (E)
Waldo (U-0)
Washington (E)
           (M)
Weir (M)
Wingra  (E)
    P
    P
    P
    N
    P
    P
    P
    P
    N-
    P-
    P
    P
 (summer)
or other?
•(in mid-1960's)
.(prior to 1960's and  in
 recent years)
EXPLANATION:
aBased on investigators' estimates:
  P=phosphorus-limited
  N=nitrogen-limited
 Investigator-indicated trophic state:
  E^eutrophic
  M=mesotrophic
  0=oligotrophic
  U=ultra
°Period during which nutrient was  specified by investigator
  to be limiting aquatic plant growth in water body,
Dash (-) = data not available.

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       Table 10. MASS RATIOS OF INORGANIC NITROGEN TO
                 DISSOLVED PHOSPHORUS IN US OECD WATER
                 BODIES
                                     Mass Ratios
                     (Inorganic Nitrogen : Dissolved Phosphorus)
                          Growing
  Water  Body              Season        Annual     Other

Blackhawk (E)a             36C            —         26e
    (NH*+NO~+NO~ as N)
Brownie  (E)               < 5.5
    (NK*+NO~ as N)
Calhoun  (E)               
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       Table 10(continued).   MASS RATIOS OF INORGANIC
               NITROGEN TO DISSOLVED PHOSPHORUS IN US
               OECD WATER BODIES
  Water Body
                                     Mass Ratios
                     (Inorganic  Nitrogen:Dissolved Phosphorus)
                          Growing
                          Season        Annual     Other
Harriet (E)
    (NH4+NO~ as N)

Isles (E)

    (NH*+NO~ as N)

Kerr Reservoir (E-M)

    (NH*+NO~+NO~ as N)
        Roanoke Arm
        Nutbush Arm
22
14
Lamb (0)
    (NH4+N03+N02 as N)
Meander CO)
       +
                    N)
Mer.dota (E)
    (NH4+N00+NO~ as N)
28
11
                                                      20
Michigan

    (NH*+NO~+NO~ as N)

        Near shore (M)     --           >100
        Open waters (0)    —            170

Minnetonka (E-*-M) Nitrogen Concentrations Not Determined

Potomac Estuary (U-E)

    (NH*+NO:+NOT as N)
       H   o   L.
        Upper Reach      2-16

        Middle Reach     1- 4

        Lower Reach      1-15

Redstone (E)

    (NH%NO"+NO~ as N)     38°            --        100€
       4 '   o   <-
     (June-
      Sept)

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Table 10 (continued).  MASS RATIOS OF INORGANIC
         NITROGEN TO DISSOLVED PHOSPHORUS IN US
         OECD WATER BODIES

Mass Ratios
(Inorganic Nitrogen :Dissolved
Growing
Water Body Season Annual
Sallie (E)
(NH*+NO~+NO~ as N)
432
1972 4 3
1973 1
Sammamish (M)
(NO'+NO^ as N) 60 30
Shagawa (E)
(NH*+NO~+NO~ as N) 8 8
Stewart
(NH.+ +NO~+NOr as N) 108°
432
Tahoe (U-0)
(NHjt+NOl + NO" as N)
432
1973 > 2 > 4
1974 > 1
East Twin
(NH*+NOr+NO: as N)
432
1971 (E) -- 27
1972 (E) -- 19
1973 (E) — 21
West Twin
(NH. +N0~+N0l as N)
432
1971 (E) -- 28
1972 (E) -- 13
1973 (E) -- 14
Twin Valley (E)
(NH*+NO~+NO~ as N) 23C
Virginia (E)
(>,-H^NO-+NO- as II) 7C
Phosphorus)
Other



3f
—

--

8f

205e




—
—



—
—
—



—
—
--

27e

55e

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       Table  lO(continued).  MASS RATIOS OF INORGANIC
               NITROGEN TO DISSOLVED PHOSPHORUS IN US
               OECD WATER BODIES
                                     Mass Ratios
                     (Inorganic Nitrogen:Dissolved Phosphorus)
                          Growing
  Water  Body              Season        Annual     Other

Waldo (U-0)
    (NH*+NO~+NO~ as N)     __            < 2
Washington
    (NH*+NO~+NO~ as N)
        1933 (E)           37              2
        1957 (E)           21             60
        1964 (E)           11              8
        1971 (M)           13             30
Weir (M)
    (NH*+NC~+NO~ as N)      2              3
Wingra (E)
    (NH*+NO~ as N)          1?             16
EXPLANATION
 Investigator-indicated trophic state :
    E = ecutrophic
    M = mesotrophic
    0 = oligotrophic
    U = ultra
 (NH4+NO~+NO~as N)-nitrogen fractions considered in N:P
                   mass ratio  calculations.
 Summer epilimnetic concentration.
"Summer surface concentration.
el!ean winter concentration.
£
ASpring overturn concentration.
 Dash (-) indicates no data available.

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Aquatic Plant Limitation in US OECD Water Bodies

     Using algal assay procedures in most cases, the majority
of the US OECD investigators characterized their respective
water bodies as being phosphorus-limited (Table 9).  The excep-
tions to this were ultra-oligotrophic Lake Tahoe (nitrogen-
limited) and the ultra-eutrophic Potomac Estuary (nitrogen-
limited in the upper and middle portions of the estuary, at
least in the summer months).  In addition, Lake Washington was
considered nitrogen-limited in the mid-1960Ts, prior to diver-
sion of domestic wastewaters;  it now appears to be phosphorus-
limited.  Ultra-oligotrophic Lake Waldo has been shown to be
phosphorus-limited in in situ primary productivity experiments
(Powers e_t al. , 1972).  However, Miller e_t al. (1974) were
unable to increase algal productivity in laboratory algal assays
with either phosphorus additions alone or phosphorus plus
nitrogen additions.  Lake Michigan is believed to be nitrogen-
limited in some nearshore areas with restricted circulation,
such as southern Green Bay  (Lee, 1974a).  The Kerr Reservoir is
reported as being nitrogen-limited in its two upper arms, but
shifting to phosphorus limitation as one moves toward the lower
ends of both arms.  Data for computing the N:P ratios were
unavailable for some water bodies (e.g., Brownie, Calhoun,
Cedar, Dogfish, George, Harriet, Isles, Lamb, Meander and
Sallie).  However, with the exception of Lakes George and
Sallie, the nitrogen budgets of the above-listed water bodies
were not determined by their respective US OECD investigators,
implying these water bodies are phosphorus-limited.  This
implication may or may not be true and may reflect the biases
of the investigators for these water bodies.

     The inorganic nitrogen:soluble orthophosphate mass ratios
of the US OECD water bodies, on both an annual and growing
season basis, were presented in Table 10.  Examination of this
table shows that, in general,  the limiting nutrient  designated
by the US OECD investigators for their respective water bodies
was substantiated by the inorganic nitrogen:soluble ortho-
phosphate mass ratio in the water bodies.

-------
     There were, however, a few exceptions to this observation.
For example, Lakes Shagawa and Weir have both annual and growing
season inorganic nitrogen:soluble orthophosphate mass ratios of
8 or less.  Yet both these water bodies are phosphorus-limited,
according to their respective investigators (Table 9).  These
discrepancies can be explained to some degree by noting when the
ratios were determined.  The period during which the ratio is
measured clearly will influence the results obtained.  This is
best exemplified with the mass ratios for Lake Mendota.  Its
annual inorganic nitrogen:  soluble orthophosphate mass ratio of
5 indicates that the lake should be nitrogen-limited.  Yet,
algal assay studies during the summer months clearly show Lake
Mendota to be phosphorus-limited during that period.  Inorganic
nitrogen:soluble orthophosphate mass ratios determined during
the summer months would also have indicated a phosphorus-limited
water body.

     Ultra-eutrophic Lake Sallie has an inorganic nitrogen:soluble
orthophosphate mass ratio of 3 or less during all times of the
year, indicating nitrogen limitation.  According to Neel (1975),
phosphorus did not seem to limit algal growth "beyond a certain
point," in Lake Sallie, implying nitrogen limitation.  Vollen-
weider (1975a; 1976a) has also reported that, even though phos-
phorus may initially be limiting algal growth, nitrogen may become
limiting beyond a certain advanced level of eutrophication.
Miller et al. (1974), studying primary productivity in 49 water
bodies,~reported that, in general, phosphorus limitation decreas-
ed in the water bodies as the primary productivity index increas-
ed.  Vollenweider (1975a) has presented evidence that this shift
to nitrogen-limitation may be due to increasing denitrification
in highly eutrophic water bodies.  According to Vollenweider
(1975a; 1976a), this point is reached when the nitrogen residence
time:phosphorus residence time ratio in the water body drops be-
low a value of one.  The nitrogen residence time:phosphorus resi-
dence time ratio , therefore, also offers a simple method for de-
dermining the aquatic plant growth limiting nutrient in a water
body.  With specific reference to Lake Sallie, another factor
which should be considered in determination of its limiting
nutrient is that its excessive aquatic plant growths are manifested
mainly in macrophyte growths.  The application of the N:P ratio
concept to Lake Sallie is likely not valid because it would
not account for that portion of the nutrients obtained through
macrophyte root systems in the sediments.  The mean inorganic
                               48

-------
nitrogen:soluble orthophosphate mass ratios in Table 10 indicate
the Kerr Reservoir to be phosphorus-limited during all times of
the year.  However, Weiss and Moore (1975)  reported that  the Kerr
Reservoir is initially  nitrogen-limited in the upper ends of
both arms, and shifts to phosphorus-limitation as one moves
toward the lower ends of the two arms (Table 9).  This inconsis-
tency may be due to the fact that the upper ends of both arms of
the Kerr Reservoir receive heavy sediment loads.  Weiss (1977)
has indicated that there may be a considerable degree of adsorption
of phosphate on the clays of the heavy sediment load, producing
low phosphate concentrations in the upper ends of the two arms
and resulting in nitrogen-limitation.  According to Weiss, this
may illustrate a problem of assessing limiting nutrients in
waters which have frequent incursions of Fe- and Al-rich sediments.

     In summary, the use of the N:P ratio approach to estimate
potential algal growth limitation by nitrogen or phosphorus re-
quires examination of this ratio over the annual cycle.  Particu-
lar attention should be given to those periods of the year when
excessive planktonic algal growth causes significant water dete-
rioration.  For many water bodies this usually corresponds to
the summer months, when the water body is being extensively used
for recreational purposes.  It is not the limiting nutrient over
the annual cycle that is of importance in determining what nutri-
ent should be considered in remedial treatment of the nutrient
loading to a water body.  Rather, the growing season is the
period of primary concern, since algal growths during the non-
growing season are seldom of consequence in terms of eutrophica-
tion control in natural waters.  Also, algal growths may be
limited by one nutrient during the summer months, or the growing
season, and another nutrient over the annual cycle.  As mention-
ed earlier, Lake Mendota exhibited such a trend.

     Attention should be given to the forms of the nutrients
available for algal growth rather than the total element content ,
since the algal growth in a water body at any given time is
limited by the algal-available ni^rogen and phosphorus forms in
the water body rather than the total nutrient content.  Caution
should be used in estimating nitrogen or phosphorus limitation in
situations where the inorganic nitrogen:soluble orthophosphate
ratio in the water body is near the normal stoichiometric ratio of
algae (atomic N:P ratio of 16:1 or mass ratio of 7.2:1) because
both nitrogen and phosphorus concentrations are in a constant
state of change.  A particular ratio that exists at one time may
be markedly altered by the different rates of supply of the
available forms of these elements from both internal and external
sources and their utilization or transformation to available
forms.

-------
     Even with the above-mentioned limitations,  the use of the
inorganic nitrogen:soluble orthophosphate ratio  represents a
reasonably accurate  method for determining the limiting nutrient
in a water body.  This chemical approach for determining the limit-
ing nutrient in natural waters is likely to be less expensive
than bioassay procedures and will yield equally  meaningful re-
sults in predicting algal growth potential when  interpreted
properly.  Further,  bioassay procedures do not take into account
many of the factors  that would influence the availability of
nitrogen and phosphorus in a water body.  In addition to the re-
sults of the US OECD water bodies in promoting this approach,
Lee (1973) has reported that the use of the inorganic nitrogen:
soluble orthophosphate ratio in determining the  limiting nutrient
has also worked reasonably well in Lake Superior and the lower
Madison, Wisconsin,  lakes.  When proper precautions are exercised
in determination of this ratio, it represents a  relatively simple
method for making reasonable predictions as to what nutrient
(i.e., nitrogen or phosphorus) is likely to limit algal growth
in most natural waters.


APPROACHES USED IN US OECD EUTROPHICATION STUDY

Initial Vollenweider Phosphorus And Nitrogen Loading Diagrams

     Although nutrient loading and nutrient concentration are
related, it is recognized that the nutrient concentration actual-
ly controls the al'gal and, -to some extent, macrophyte standing
crops in a given water body, and thereby the eutrophication
process.  However, many factors directly and indirectly affect
the relationship between nutrient loading and the resultant
nutrient concentration (Vollenweider, 1968).  Furthermore, from
the point of view of eutrophication control, the nutrient load-
ing to a water body is more easily managed than the nutrient
concentration within a water body.  It was the loading approach
that was adopted for the US OECD eutrophication study.

     Sawyer (1947) was among the first to use the concept of
nutrient loading in his studies of the effects of agricultural
                                50

-------
and urban drainage and wastewaters on the fertility of the Madi-
son, Wisconsin, lakes.  He made the observation that the lake
which received the greatest quantity of phosphorus and nitrogen
on an areal basis experienced the most frequent and most severe
algal blooms.

     Rawson (1955) and Edmondson (1961) emphasized the importance
of mean depth  (a measure of the volume related to unit surface
area) to the productivity of water bodies.   In any evaluation of
areal loading, this parameter took into account the degree of
dilution and its effect on the nutrient concentrations in deeper
bodies of water.  Inclusion of mean depth in the evaluation of
productivity also allowed for the role of the thermocline in in-
fluencing nutrient recycling from sediments (Stauffer and Lee,
1973).

     Vollenweider (1968) quantitatively defined the relationship
between nutrient loading and planktonic algal trophic response and
devised a loading relationship based on these components.  When
Vollenweider plotted the surface area total phosphorus loading
(g P/m2/yr) or total nitrogen loading (g N/m^/yr) versus the
mean depth (m) on a log-log scale, he found that water bodies of
similar trophic states appeared in the same general areas of the
diagram (Figure 5).  This same relationship was also derived for
nitrogen loadings (Figure 6), assuming algal nitrogen require-
ments were related to phosphorus requirements in the ratio of
15:1 by weight.  According to Vollenweider (1977), while this is
about twice the mass ratio generally accepted, he felt this high
N:P ratio applied to loading (not concentration) appeared to be
more appropriate, and probably included effects of denitrification
which reduces  the available nitrogen (in terms of concentration)
relative to phosphorus.  Boundary loading conditions, theoretically
based on Sawyer's spring overturn critical nutrient concentrations
(Vollenweider, 1968; Vollenweider and Dillon, 1974), were incor-
porated into the diagrams, which grouped the lakes into the three
standard trophic states (i.e., oligotrophic, mesotrophic and eu-
trophic).   The lower bounary line ("permissible") designated the
maximum phosphorus or nitrogen loading levels, as a function of
mean depth, that a given water body could tolerate and still retain
its oligotrophic character.  The upper boundary line ("excessive")
represented the phosphorus or nitrogen loading level, as a function
of mean depth, above which a given water body would be characterized
as eutrophic.   The zone separating the oligotrophic and eutrophic
categories represented the mesotrophic category.  This was consid-
ered a transition zone between the oligotrophic and eutrophic cate-
gories .

     The approximation for the permissible loading boundary
condition was  empirically determined to be

                  LC(P) = 25 z°'6                       (1)


                               51

-------
   10
 a
o
z
o
s
CO
D
tr
o
i
a.
to
o
i
a.
p
  0.01
         EUTROPHIC ZONE
               NO
                                  MESOTROPHIC ZONE

                                                        X
                                                        X
                         E»    W   Z

                          MA«
                         •     /»AN  X
                          • T  X     X
                          X    X
      EXCESSIVE LOADING
              V
     X
          X
                          LOADING
                                                TA
                                  OLIGOTROPHIC ZONE
       (FROM VOLLENWEIDER.I968)
                      10
                                         100
                                                          1000
      KEY TO LAKES

     TA-TAHOE
      A- AEGERISEE
      V - VANERN
      L -LE.MAN
      0-ONTARIO
     BO-CONSTANCE
     AN-ANNECY
                        MEAN   DEPTH (m)
                       MA-MALAREN
                        T-TURLERSEE
                        F-FURES
                        S- SEBASTICOOK
                        H-HALLWILERSEE
                       MO-MOSES
                       MO- NORRVIKEN
E - ERI E
P- PFAFFIKERSEE
G- GRIEFENSEE
B-BALDEGGERSEE
W- WASHINGTON
2- ZURICHSEE
Figure  5.   Vollenweider's  Total   Phosphorus  Loading
              and Mean  Depth  Relationship.
                            52

-------
1000
o«IOO

o
z
Q
LJ
O
O
o:
  10
I-
o
        EUTROPHIC
          ZONE
                                              I FROM VOLLENWEIDER.I968)
 > MAL
                                WA
L- N
                               PERMISSIBLE
                                LOADING
                          ^MESOTROPHIC
                              ZONE
                          OLIGOTROPHIC
                              ZONE

                               TA
                        10
     KEY TO LAKES

     P- PFAFFIKERSEE
     Z- ZUR1CHSEE.UNTERSEE
     H- HALLWILERSEE
     B-BODEN-OBERSEE
                          MEAN  DEPTH (m)
       ZE-ZELLERSEE
      MAL-MALERN
        N- NORRVIKEN
      L-N LOUGH NEAGH
                                            100                  1000
MEN - MENDOTA
 TA -TAHOE
 WA - WASHINGTON ( 1957)
 Figure 6.   Vol1enweider's  Total  Nitrogen  Loading  and
              Mean  Depth  Relationship.
                              53

-------
           where L (P)  = areal permissible total phosphorus
                  c      loading (mg P/m2/yr);  and

                     z  = mean depth (m).

The excessive loading boundary condition  was considered to be
approximately twice the permissible loading (Sakamoto, 1966;
Vollenweider, 1968; 1976a; Dillon, 1974a; Dillon and Rigler,
1974a) as follows : '
                 L(P)  = 50 z0>6                       (2)
           where L(P)  = areal excessive  phosphorus loading
                         (mg P/m2/yr)
Assuming an N:P loading ratio of 15:1 by  weight (Vollenweider,
1968), then the permissible and excessive loading lines, respect-
ively, for nitrogen are determined by similar reasoning as:
                 L(N)  = (15) (25 or 50)  z°'6          (3)
                                                      2
           where L(N)  = areal nitrogen loading (mgN/m /yr).

The slope of the boundary lines indicated the greater dilution
capacity of deeper water bodies, which influences their ability to
assimilate more nutrients than shallower  lakes without increasing
their degree of fertility.  A water body's relative degree of
eutrophy or oligotrophy on either loading diagram was proportionate
to its vertical displacement above or below the "permissible"
loading line.  Thus, in Figure 5, Lake Moses is relatively four
times more eutrophic than Lake Sebasticook in terms of phosphorus
loadings.  Likewise, Lake Aegerisee is relatively more oligotrophic
than Lake Vanern, based on their respective phosphorus loading and
mean depth characteristics (Vollenweider  and Dillon, 1974).

     This model marked a significant advance in eutrophication
studies and became widely accepted as a guide to the degree of
eutrophy of a given water body.  It was the first credible quan-
titative guide to  "permissible" and "excessive" phosphorus and
nitrogen loading levels for lakes and impoundments.  That is,
for most of the water bodies for which sufficient phosphorus
loading data were available, the trophic  state predicted by the
Vollenweider loading diagram agreed with the trophic state in-
dicated by the standard, but arbitrary, indicators available at
the time (e.g., nutrient concentrations,  chlorophyll concentra-
tions, primary productivity, Secchi depth, hypolimnetic oxygen
depletion, etc.).

     The Vollenweider phosphorus loading  diagram was subsequently
used in a number of studies to describe or predict the degree of
eutrophy in various waters as a function of phosphorus loadings.
For example, the International Joint Commission (1969) and
Patalas  (1972) used it to describe the trophic conditions of the


                               54

-------
 Great  Lakes.   Schindler  and Nighswander  (1970) used  it  to  describe
 Experimental  Lake  227  in their nutrient  enrichment studies  in  north-
 western  Ontario.   In fact, it still  appears  in the literature  in
 this form  even today.

     However,  Vollenweider (1968;  1975a)  stated that his initial
 phosphorus and nitrogen  loading  diagrams  were only approximate
 relationships  and  that other parameters would also have to  be
 considered in  establishing a water body's trophic status.   These
 factors  included the extent of shoreline  and littoral zone,  degree
 of  nutrient mixing in  the water  column,  internal loading from
 the sediments,  and especially water  renewal  time (Vollenweider
 and Dillon, 1974). Vollenweider (1975a)  noted that  his initial
 model, though  it worked  reasonably well  for  hydraulic residence
 times  of several months, did not account  for the situation  that
 two water  bodies could have identical mean depths , but  different
 hydraulic  residence times.  Water bodies  with shorter hydraulic
 residence  times (i.e., faster flushing rates) would  also have  fast-
 er  cycling of  water through the  systems.   A  water body  with a  fast-
 er  flushing rate could assimilate a  larger nutrient  loading, with
 no  adverse eutrophication responses , than a  slower flushing lake
 because  of a  generally faster nutrient washout which could  result
 in  a "short-circuiting"  of input nutrients before they  have had
 sufficient time to interact with the algal populations  in  the  fast-
 er  water body.   Edmondson (1961; 1970a)  pointed out  that a  lake
 receiving  nutrients supplied in  a diluted form (such as land runoff)
 would  be affected  differently than one receiving its nutrients in
 a concentrated form (such as domestic sewage inputs).

     Dillon (1974a, 1975) was the  first to report water bodies
which did not fit  Vollenweider's original phosphorus loading dia-
gram scheme.  In his study of the phosphorus budgets of nineteen
southern Ontario lakes, he found a number of them had phosphorus
loadings and mean  depth characteristics which would place them
in Vollenweider's  eutrophic category on his loading diagram
 (Figure  5); yet they also had large Secchi depths,  low chlorophyll
concentrations and no  significant hypolimnetic oxygen depletion.
Dillon attributed  this discrepancy to the fact that the ratios
of their drainage  areas to surface areas were very large.   This
factor and their low mean depths gave them very high flushing
rates.   Dillon concluded the  anomalous  fit of these water bodies
on the Vollenweider phosphorus loading diagram was  a result of
their rapid flushing rates.

Vollenweider Phosphorus Loading and Nitrogen Loading
Versus  Mean Depth/Hydraulic  Resjdence 'Time Relationships

     In an  attempt  to  allow  for  the effects of fast  or  slow flush-
ing rates on the nutrient loading-trophic response  relationships
in natural  waters,  Vollenweider  (1975a;  1976a; Vollenweider and
Dillon, 1974)  modified  his phosphorus loading diagram to include
the hydraulic  residence time  (i.e., water body volume/annual


                                55

-------
inflow volume).  This modification was based on an input-output
model involving the behavior of non-conservative substances in
water bodies (Vollenweider,  1975a, Dillon, 1974b).  This modifica-
tion allowed the effects of hydraulic loading (as contrasted to
nutrient loading) to be included along with the nutrient loading
and morphometry parameters of his initial loading diagram.

     Vollenweider focused his attention on modifying only the phos-
phorus loading diagram.  He singled out phosphorus for attention
because it is generally believed to be the aquatic plant nutrient
most frequently controlling eutrophication in natural waters
(Sawyer, 1966; Fruh et al.,  1966; American Water Works Association,
1966; 1967; Vollenweider, 1968; 1975a; 1976a; Lee, 1971; 1973;
Likens, 1972a; Vallentyne, 1974; Vollenweider and Dillon, 1974; US
EPA, 1976a; 1976b).  Furthermore, the phosphorus input to a water
body is usually technologically easier to control than the nitrogen
input.  Much of the phosphorus supplied to water bodies is intro-
duced by way of point sources, such as in domestic or industrial
sewage.  Nitrogen, while supplied from point sources, is often also
introduced in large quantities from non-point (diffuse) sources,
such as land runoff, precipitation, dry fallout and nitrogen fixa-
tion.  These diffuse sources are usually far more difficult and
expensive to control.  In general, then, it is believed that the
control of phosphorus loading' to a water body is technically and
economically more feasible than control of nitrogen loading.  Con-
sequently, Vollenweider focused on modifying his phosphorus load-
ing diagram.  Vollenweider's approach of concentrating on the phos-
phorus loadings to water bodies was recently given support by the
general assemblies of both the International Limnological Congress
and the International Ecology Congress, both of which unanimously
passed resolutions recommending widespread phosphorus control as a
solution to eutrophication (Schindler, 1977).

     Vollenweider (1975a; 1976a) modified his relationship to in-
clude the hydraulic residence time.  In this report, the hydraulic
residence time is defined as the ratio of the water body volume
(m^) to the annual inflow volume  (m^/yr) and represents the lake
filling time.  The hydraulic residence time could also have been
defined as water body volume divided by annual outflow volume
since the majority of the US OECD water bodies are in the north-
central and northeastern US.  It is generally held that precipitation
and evaporation are approximately equal over the annual cycle in
these areas.  Thus, the hydraulic residence times, computed using the
inflow volumes would presumably not be significantly different from
those obtained using the outflow volumes (the importance of this par-
ameter was recently illustrated by Piwoni et al.  (1976) in their
evaluation of the trophic state of Lake Michigan.  Two different hy-
draulic residence times were computed, depending on whether outflow
alone or outflow plus deep return flow during stratification were
considered in the computations.  The reader is referred to Piwoni
et al. (1976) for a detailed discussion of this problem).  Vollen-
weider Ts modification was to plot a water body's areal total phos-
phorus loading (g P/m^yr) versus its ratio of mean depth (m) to

                                 56

-------
hydraulic residence time (yr) .   This ratio was represented as
Z/TW.  With this relationship,  the critical phosphorus loading of
comparable lakes is directly proportional to their mean depths, and
indirectly proportional to their hydraulic residence times.  The
direct proportionality of the critical phosphorus loading to the
mean depth relates to the dilution of the phosphorus input by the
water body volume.  The reciprocal proportionality of the critical
phosphorus loading to the hydraulic residence time relates to the
likely residence time of the input phosphorus in the water body.
It was apparently Vollenweider ' s intent that the variables of mean
depth and flushing rate be considered in this modification.  However,
Z/TW equals the hydraulic load,  qs (m/yr) , per unit water body
surface area.  Thus, it appears  that mean depth, as an independent
parameter, is lost in part.  Vollenweider ' s phosphorus loading versus
mean depth/hydraulic residence time relationship is presented
graphically in Figure 7.  As with Vollenweider ' s original phosphorus
loading diagram (Figure 5) phosphorus boundary loading lines based
on Sawyer's (1947) critical nutrient concentrations, and represent-
ing the permissible and excessive phosphorus loading levels , have
been drawn into Vollenweider ' s modified phosphorus loading diagram.
According to Vollenweider (1976a), from a simple inspection of lakes
plotted using this modified approach, the phosphorus loading criteria
for separating oligotrophic from eutrophic lakes was as follows :

                 L (P) = (100)  (Z/T )°'5             (U)
                  C                CO

          where L (P)  = areal permissible total phosphorus
                         loading (mg
                     z = mean depth (m) , and

                    T  = hydraulic residence time = water body
                         volume (irr) /annual inflow volume (m^/yr).

As before, the excessive phosphorus loading was assumed to be
equal to twice the permissible loading (Sakamoto, 1966; Vollen-
weider, 1975a, 1976a; Dillon, 197"4a).   Thus water bodies
plotting above the excessive loading line are generally eutrophic
while those plotting below the permissible loading line are
generally oligotrophic, based on their phosphorus loadings and
mean depth/hydraulic residence time characteristics.  A detailed
derivation of this approach is presented in Vollenweider  (1975a).

     It is this version of Vollenweider ' s model which was proposed
by the US EPA (1975b, 1976a) as a basis for determining critical
phosphorus loadings for US lakes and impoundments .  A further
modification of Vollenweider ' s model involves the position of
the permissible and excessive loading lines in his loading dia-
gram.  This new modification, in the opinion of these reviewers,   .
                               57

-------
      10
               EUTROPHIC
                  ZONE
                                                                           EXCESSIVE
                                                                            LOADING
    e
    •x.
    QL
                                                   •PERMISSIBLE
                                                    LOADING
en
CO
    ID
    2

    O
    <
    O
    cr
    o
    CO
    O
    X
    0.
     0.
    0.01
       0.
                                                                         OLIGOTROPHIC
                                                                            ZONE
           (FROM VOLLENWEIDER, I975a)
                            I
                                                                     I
    I        _           10                   100
MEAN  DEPTH,Z/HYDRAULIC RESIDENCE TIME,TO, (m/yr)
               Figure  7.
   Initial  Vollenweider  Total Phosphorus Loading and
   Mean  Depth/Hydraulic  Residence  Time Relationship.
1000

-------
marks a further refinement of Vollenweider's approach for deter-
mination of critical phosphorus loadings for lakes and impound-
ments.  The derivation of this new modification is presented in
the following section.

     Based on earlier work by Biffi (1963) and Piontelli and
Tonolli (196^), Vollenweider (1975a; Dillon, 197i+b) developed a
mass balance model for total phosphorus in natural waters.  As
such, it was an accountability model concerned with the balance
of phosphorus between its sources and sinks.  In addition to the
initial mean depth parameter, this model included terms for the
hydraulic residence time and a sedimentation parameter.  Vollen-
weider 's model indicated that the phosphorus dynamics of a water
body can be expressed as:


              d[P]/dt r Phosphorus Load minus Outflow Loss minus
                        Sedimentation Loss


                      =  (Zu.[P]./V) -a[P]-pw[P]         (5)


                                                                _3
           where  [P] = lake total phosphorus concentration (ML  ) ,

                   u. = flow rate of the jth tributary (L3?'1),

                  [P]. = phosphorus concentration in j   tribu-

                        tary (M L~3),
                                      3
                   V  = lake volume (L ),

                   p  = hydraulic flushing rate (= annual inflow
                        volume/lake volume) (T  ),  and
                                                               _]_
                   a  = phosphorus sedimentation coefficient (T  ).
                    P

Vollenweider assumed a completely mixed reactor model of constant
volume in which the outflow phosphorus concentration was equal to
the in-lake phosphorus concentration.   He further assumed the
water body had equivalent inflow and outflow rates and that there
was no internal loading of phosphorus  to the water column from
the sediments.  He also assumed that phosphorus sedimentation
was proportional to the phosphorus concentration in the water
body, rather than to the phosphorus loading.
                                59

-------
    The time-dependent solution to this model is:


 [PL ~- CPL e-(pw%)(t-to) + (£(P)/(p +a ))(l-e-(pa)+ap)(t-to)
    T-      "-o                          to  p
                                                          (6)


The steady state solution (i.e., t->») to this model (Vollen-
weider, 1975a; 1976a) is


                 (pw + ap)                                (7)
     where [P]^ = steady state total phosphorus concentration

                  (M L~3) , and


           HP) = volumnar phosphorus loading

                  (M L~3 T'1) = SU.[P]./V


Now, £(P) = L(P)/z, where L(P) = areal total phosphorus loading
and z = mean depth.  Therefore, Equation 7 above becomes
     [P]  = L(P)/(z(p  + a ))
        00            ti)    p
Equation 8 can then be arranged as
     UP) = [P]w •  i(pu + ap).                            (9)
          can be taken for simplicity as Sawyer's (1947)    ^
critical spring overturn phosphorus concentration of 10 mg/m  .
The hydraulic flushing rate, pw , is equal to I/hydraulic
residence time (= I/TW) .  The phosphorus sedimentation rate
coefficient, ap, cannot easily be measured directly.  However,
Vollenweider (1975a; 1976a) has indicated as a general rule
that c?p can be approximated by


     a  = 10/z.                                          (10)
      P
Thus, Equation 9 becomes
                              60

-------
Lc(P) =
                                 ap)
                 = (10 mg/m3)(z/Tw + z (10/z))
      = 100 +(10 (Z/T ))
                     to
                                                        (11)
     where L (P)
            c
               OJ
        areal permissible total phos-
        phorus loading (mg  P/m?/yr),
              z  = mean depth (m),
        hydraulic residence time (yr) = lake
        volume (m^) /annual inflow volume (rn^/yr) , 2nd
              ]   = critical concentration of total phosphorus at
                   spring overturn = 10 mg/rn^.
As with the earlier model, the excessive phosphorus loading
boundary condition was considered to be approximately twice the
permissible loading (Sakamoto, 1966; Vollenweider, 1968; 1976a;
Dillon, 1974a; Dillon and Rigler; 1974a).  Thus, the equation for
the excessive loading line becomes
            L(P) = 200 + (20
                                             (12)
     where  L(P) = excessive phosphorus loading  (mg P/m^/yr).
These equations, theoretically based on Sawyer's (1947) critical
spring overturn phosphorus concentration, serve as the basis for
the modified phosphorus loading and mean depth/hydraulic
residence time diagram presented in Figure 8.  Vollenweider's
modified phosphorus loading diagram (Figure 8) indicates that
below a certain combination of mean depth and flushing, the
phosphorus loading tolerance of a given water body becomes con-
stant in spite of the fact that, based on mean depth alone,
water bodies may appear to have a higher assimilation capacity.
This is not indicated in his previously reported loading diagram
(Figure 7).  In this new modified phosphorus loading diagram,
the boundary lines flatten out at Z/TW values of <2.   In addi-
tion, at Z/T^ values >80, the tolerable loading capacity becomes
proportional to Z/T^, which is contrary to what was found with
his original model  (Figure 7).

                               61

-------
      10
CD
ro
     E
    CL
     a>

    O
    z
    Q
    <
    o
    (T
    O
    I
    o
    I
    Q.
     0.01
             EUTROPHIC
               ZONE
                            / LOADING
                                                                      /     /PERMISSIBLE
                                                                    /     /  LOADING
     X
        X
          ( FROM VOLLENWEIDER, I975o )



         	I	I  I  I  I 1  I I I
                       OLIGOTROPHIC
                           ZONE
                  Mil
                                                                                     I I I I
       0.1
10
                        MEAN DEPTH,Z/HYDRAULIC RESIDENCE TIME,TW
100
( m/yr)
1000
               Figure  8.   Modified  Vollenweider  Total  Phosphorus  Loading  and
                           Mean Depth/Hydraulic Residence Time  Relationship.

-------
                                           —   -
     A total nitrogen loading (i.e., NHt+N0,.+N0? + organic
nitrogen) and mean depth/hydraulic residence time diagram
has also been prepared for analysis of the US OECD eutrophica-
tion study data.  The nitrogen loading diagram is identical in
form to the phosphorus loading diagram except that it contains
no permissible or excessive loading lines.  The criteria for
the positioning of the permissible and excessive boundary lines
are currently being derived for water bodies which are nitrogen-
limited, or which can be made nitrogen-limited with respect to
aquatic plant nutrient requirements.  The development of the
permissible and excessive loading boundary conditions is neces-
sary so that the type of relationship developed by Vollenweider
for examining the trophic conditions of water bodies based on
their phosphorus loadings and mean depth/hydraulic residence
time characteristics can be applied to water bodies which are
nitrogen-limited.

Emphasis on Phosphorus Loading Relationships

     Vollenweider has continued to modify and improve his phos-
phorus loading relationships during the past several years.
Moreover, others (Dillon, 1975; Larsen and Mercier, 1976) have
proposed additional parameters to be considered in any evalua-
tion of a water body's productivity and general trophic condi-
tion.  These new models, to be used later in this report, are
discussed in the following sections.

     In all subsequent loading diagrams in this section, at-
tention is given mainly to phosphorus loading relationships.
Relationships between nutrient loadings and water body trophic
response and water quality parameters are explored in later sec-
tions of this report.  However, all the loading diagrams in this
section relate phosphorus loadings to either influent phosphorus
concentrations, chlorophyll concentrations or retention coeffi-
cients.  The originators of the various loading diagrams them-
selves derived their loading-response relationships only for
phosphorus loadings.  Vollenweider  (1975a) reported his concen-
tration on phosphorus loadings stemmed from "...the relatively
scant knowledge we have about other factors, e.g., nitrogen."
In addition, the majority of the US OECD water bodies were
characterized as being phosphorus-limited with respect to
aquatic plant requirements.  Consequently, all the subsequent
loading diagrams refer to phosphorus loadings.  It is assumed
that the same relationships could be derived for nitrogen load-
ings.  However, the originators of the subsequent loading dia-
grams made no attempt to do so.

Vollenweider Critical Phosphorus Loading Equations

     Concurrent with his phosphorus loading diagrams, Vollen-
weider derived additional methods for calculating critical
                               63

-------
phosphorus loadings to water bodies.  The first approximations
(Vollenweider, 1976a) of the critical phosphorus loading range
were given earlier in Equations 1, U- and 11.  Water bodies re-
ceiving a phosphorus loading below this permissible phosphorus
loading estimate  (Figures 5, 7 and 8) would be considered oligo-
trophic, while water bodies receiving at least twice this per-
missible loading would be considered eutrophic (Vollenweider,
1976a; Vollenweider and Dillon, 1974-).

     Vollenweider (1976a) has derived a more general relation-
ship from Equation 9.   Vollenweider (1976a;  Sonzogni et al. ,
1976) has incorporated the concept of phosphorus  residence" time
as a reference parameter for determining critical phosphorus
loads.   Vollenweider has included this parameter in this refine-
ment of his critical phosphorus loading equation in an attempt to
compensate for the loss of mean depth as an  independent criterion
for assessing the effects of phosphorus loading on a water body.
According to Vollenweider (1976a), the concept of phosphorus
residence time can be approximated in the same manner as the
hydraulic residence time, or theoretical filling time, of a water
body (i.e., T  = water body volume/annual inflow volume).
Determination of the residence time of any substance entering
a water body requires only -the knowledge of  the loading of that
substance to the water body and the mean concentration of that
substance in the water body during the same  time  interval.  Thus,
for phosphorus

             T  =[p]./£(P)                            (13)
              P     *


       where T  = phosphorus residence time  (T),

            [PL= mean in-lake phosphorus concentration (M L~ )  and

                                                  -3  -1
           £(P) = volumnar phosphorus loading (ML   T  ).


Equation 13 defines the hypothetical time necessary to bring the
phosphorus concentration of a water body to  its present level
starting from a zero phosphorus concentration in the same manner
that the hydraulic residence time, as used in this report, de-
fines the theoretical "filling time" of a water body.  This same
approach was used by Sonzogni et al.  (1976)  in development of a
phosphorus residence time recovery model.  This model will be
discussed in a later section of this report.

     However, Vollenweider (1976a) has noted that the phosphorus
loading is not independent from the hydraulic loading.  The only
exception to this observation would be instances where the phos-
phorus loading is a direct input(s) of high  concentration, and

                                64

-------
thus only marginally accounts for the total hydraulic loading.
Therefore, Vollenweider concluded that it would be more meaning-
ful to consider the phosphorus residence time relative to that
of water.

     Therefore,

          TT  = T /T  = ( [PL /£(P))/(V/Q)                (14)
           r    p  to       A
                     -- [PL/[P].
        where  TT  = phosphorus residence time relative to
                    hydraulic residence time  (T T"-'-),

               T  = hydraulic residence time  (T);
                                  3
               V  = lake volume (L )?

                                     3  —1
               Q  = inflow volume (L  T  ),
                                                              _ Q
              [P].= mean inflow phosphorus concentration  (ML  ) and
                                                               _ 2
              [P] = mean in-lake phosphorus concentration  (M  L   ).
                 A

In analyzing the dependence of tp on T^ for a wide range  of water
bodies, Vollenweider (1976a) has noted that Tp/Tw is neither  in-
dependent nor inversely proportional to TW.   Rather, Tp/Tw
tends to decrease as TW increases.  He has determined that the
relative phosphorus residence time depends on the hydraulic resi-
dence time by a statistical relationship which results in the
following equation,

          TT  =T/T  =p/(p  +a)                     (15 )
           r    p  co    to   to    p


        where  p  = hydraulic flushing rate (T   ) =  1/t  ,  and

               a  = phosphorus sedimentation  coefficient  (T   )


However, Vollenweider (1976a) has also noted  that for lakes of
less than 20 m mean depth and/or rapid flushing rates this rela-
tionship between T-p/T^ and T^ cannot be linearly extrapolated
below T  <1 .
                                65

-------
An approximation which takes care of this problem is
           T /T(o = 1/C1 + 7z/qs)
                                                      (16)
Equations 15 and 16 can then be combined as follows,





           T /T  = P /(p  + a ) = 1/(1 + /T~).        (17)
            p  co    w   co    p           v  co






Equation 17 can then be solved for the sedimentation rate coef-

ficient , a  , as follows,
        '  p'






           a  = JT~/T   =  /z/q /T                      (18)
            p   Y  co  to   *  Hs  to
If this estimate of ap is inserted into Equation 9, a more

generalized relationship is obtained for determining critical

phosphorus loads which holds over the entire spectrum of combina-

tions of mean depth and hydraulic loadings.  This relationship

is derived as follows,

                 = 10  • q   (1 + /z/q  )                 (19)
                         J
     where  [P] ^ - Sawyer's  (1947) critical spring overturn

                   phosphorus concentration =  10  mg/m^,



              z  = mean depth (m),



              T  = hydraulic residence time (yr) , and



              q  = hydraulic loading  (m/yr) =  Z/T  .





                                66

-------
This equation expresses the phosphorus loading tolerance in terms
of the morphometry of the water body (condensed into the term of
mean depth, z) , and the hydrologic properties of the water body
(expressed as hydraulic loading, qs).   Thus, in principle, the
phosphorus loading tolerance of a water body can be considered
as a function of its mean depth and hydraulic loading  (Vollen-
weider, 1976a).

     This relationship has been developed by Vollenweider into
the form of two equivalent diagrams (Figures 9 and 10).  In
Figure 9, the permissible phosphorus loading, LC(P), is plotted
against mean depth and parameterized as a function of  the hy-
draulic loading, qs.  In Figure 10, LC(P) is plotted against the
hydraulic load and parameterized as a function of mean depth, z.

Vollenweider PhosphorusLoading Characteristics and Mean Epilimnetic
Chlorophyll a Re la t Ton ship"             ~~

     Equations 8 or 19 can be rewritten in terms of the relation-
ship between the phosphorus loading and the resultant phosphorus
concentration in the water body, rather than in terms of critical
phosphorus loading levels.

Recalling that p  = I/T , a  = JT 7r  and T  = z/q , Equation 8
        &       co      co'  p   V co  co      to     Hs'H
can be rearranged as follows:
          [P]  = (L(P)/q ) (1/(1 +  /z/q ))             (20)
             ^^          o              o


Equation 20, therefore, relates the predicted in-lake phosphorus
concentration (assuming a steady-state condition) to an equiva-
lent expression involving the phosphorus loading as modified by
the hydraulic load.  According to Vollenweider (1975a; I976a)
L(P)/qs represents the average inflow phosphorus concentration.
This useful relationship will be used in a later portion of this
report to check the phosphorus loads reported for the US OECD
eutrophication study water bodies.

     Several authors (Sawyer, 1947; Sakamoto, 1966; Dillon, 1974a;
Dillon and Rigler, 1974a; Bachmann and Jones, 1974; Jones and Bachmann,
1976) have shown that a relationship exists between the phosphorus
concentration at spring overturn and the mean chlorophyll con-
centrations in a water body during the following summer growing
season.  Since a positive correlation has been shown to exist be-
tween spring overturn phosphorus concentration and average summer
chlorophyll concentration in a water body, it is logical to assume
a positive correlation may exist between phosphorus loading and
average chlorophyll concentrations.  Vollenweider demonstrated
such a correlation between phosphorus loadings and chlorophyll
concentrations at the 1975 North American Project Meeting in
Minneapolis.  He plotted the phosphorus loadings of a water body,

                                 67

-------
                     10000
CD

oo
                     
-------
           10000
en
to
          E
          \
          Q.
          o>
          E


          o
          z

          o
          <
          o
         <
         o
         o:
         o
1000
 100
 Vollenweider

-  (l975°2)oo
              10
               O.I
                                                    HYDRAULIC RESIDENCE TIME
                                                    T
                                                                   (FROM VOLLENWEIDER,I976o)



                                                                 I I I I I	I   I  I  I I  I
                                       10

                           HYDRAULIC  LOAD, qfi ( m/yr)
                                                   100
1000
                   Figure 10.   Vollenweider Critical  Phosphorus  Loading  and
                                 Hydraulic  Loading  Relationship.

-------
as manifested in the_pJao.sphorus loading characteristics term
(L(P)/qs) (1/(1 +  vz/q )) in Equation 20, and the mean epi-
limnetic chlorophyll a_ concentration of the water body.  Even
though the chlorophyll a_ concentrations consist of a mixture of
annual and summer average values, Vollenweider showed a definite
relationship (r = 0.87) between the phosphorus loading character-
istics of a water body and its average epilimnetic chlorophyll a_
concentration.   Vollenweider's resulting loading diagram is pre-
sented in Figure 11.  This diagram includes confidence intervals
for prediction of chlorophyll concentrations in a water body as
a function of its phosphorus loading, as modified by its hydraulic
loading.  The reader is reminded that since the phosphorus load-
ing characteristic term  is equivalent to the predicted mean in-
lake phosphorus concentration (Equation 20), assuming a steady
state condition, Vollenweider is, in effect, relating chlorophyll
a concentrations to total phosphorus concentrations in the same
manner as other researchers (Sakamoto, 1966; Dillon, 1974a; Jones
and Bachmann, 1976).  However, Vollenweiderrs contribution was
to provide a phosphorus loading term, modified by hydraulic load-
ing, which was equivalent to the predicted  in-lake phosphorus
concentration (Equation 20).  Thus, Figure  11 indicates the re-
lationship between predicted in-lake phosphorus concentration,
as well as the phosphorus loading characteristics, and the mean
epilimnetic chlorophyll a concentrations in a water body.  In this
manner, chlorophyll a_ concentrations can be related to phosphorus
loadings, as well as to mean phosphorus concentrations.  Larsen and
Mercier  (1976) used the same phosphorus loading relationship in
shifting emphasis from phosphorus loadings  to influent phosphorus
concentrations.  This will be considered in a later section of
this report.

      It should be noted that the response  of a water body to a
reduction in phosphorus loading will not be an immediate accom-
panying reduction in the chlorophyll concentration of the water
body.  Rather, there will be a "lag period" during which the phos-
phorus concentrations, and hence, chlorophyll a concentrations, in
the water body are adjusting to the new phosphorus loadings.  When
the water body has reached a new equilibrium condition with
respect to its phosphorus concentrations, then the loading dia-
gram  (Figure 11) can validly be used to predict the expected chloro-
phyll biomass in the water body.  Vollenweider (1976a) has demon-
strated this lag phenomenon with data from  Lake Washington.  This
concept  is examined by Sonzogni et al.  (1976) in their phosphorus
residence time model, and will be explored  further in a later
section of this report.

Dillon Phosphorus Loading-Phosphorus Retention and Mean_Depth
Relationship

      Dillon (Vollenweider and Dillon,  1974; Dillon, 1975) was one
of the first to point out one of the omissions of Vollenweider's
                                 70

-------
  100
z
o
tr
H


§  10
z
o
o

01
OL
O
cr

3

o


p  I

UJ
UJ


UJ

O

<
OL

UJ
  O.I
        ( FROM VOLLENWEIDER, 19760 )


                                                           X
                                                             X
                                X   /  /
                               x    / /
                           x
     //
                 99%
                99 %
                     /
                     11
                      /
                               -LOADING CHARACTERISTICS
 WITHIN

TOLERANCE
                                                    EXCEED

                                                   TOLERANCE-
                                                   i _ i i i i i I
    O.I
                                        10
                               100
                                                                          1000
       Figure  11.
                                    (mg/m3)



                    Vol 1 enwei der Phosphorus  Loading Ctiaracteri sti cs

                    and  Mean Chlorophyll  a^ Relationship

-------
original phosphorus loading diagram (Figure 5).  Because flushing
rate and hydraulic residence time,  as  well  as  phosphorus  loading
and mean depth, play a part in determining the relative degree
of fertility of a water body, Dillon attempted to include these
parameters in a formulation of his own,

     Dillon derived his model from Vollenweider ' s original phos-
phorus mass balance model, as indicated in Equation 5.  The
steady state solution to Vollenweider ' s model (Equation 8) was
shown to be [P]OT = L(P)/(z/TW + z/a ) .  However,  as mentioned
earlier, measurement of 0p is very difficult and  only indirectly
obtainable.  Consequently, using the same assumptions as were
used to derive the model, Dillon (1975; Dillon and Rigler, 1974a)
derived an alternate parameter, the phosphorus retention coeffi-
cient, R(P) , which can be shown to have a functional relationship
to Vollenweider ' s phosphorus sedimentation rate coefficient, ap .
Dillon (1975; Dillon and Rigler, 1974a) has indicated that R(P1
can be approximated, assuming a steady state condition, as
          R(P) = 1 - (ZqQ [P]Q / Iqi [P]i)            (21)

                                  3
      where q  = outflow volume (m /yr) ,
             o
                                 3
            q. = inflow volume (m /yr),
                                            3
           [P] = outflow concentration (mg/m ) ,  and
                                           3
           [P].= inflow concentration (mg/m ).


Thus R(P) represents the fraction of the phosphorus input which
is retained in the sediments of the water body (i.e., the frac-
tion of the inflowing phosphorus which sediments annually) .
Conversely, l-R(P) is the fraction of inflowing phosphorus not
retained in the water body (i.e., it is lost by way of outflow).
Kirchner and Dillon (1975) have demonstrated that R(P) was highly
correlated with the areal water loading.  Using multiple regres-
sion analysis they have produced a regression equation for predict-
ing R(P) which is very similar to the value predicted on theoret-
ical grounds (Snodgrass, 1974; Snodgrass and O'Melia, 1975).  Chapra
(1975) has presented an interpretation of the high correlation
found between R(P) and the areal water loading and derived an al-
ternate method of determining R(P) as follows,
                                 72

-------
            R(P) = v/(q  + v)                         (22)
                       s
               u = apparent settling velocity of total phos-
                   phorus = a u ' ,

              q  = areal water load = Q/A,
               o

               <* - fraction of total phosphorus represented by
                   settleable particulate phosphorus ,

              u'  = settling velocity of settleable particulate
                   phosphorus,

               Q = lake discharge volume, and

               A = water body surface area.


Regardless of how it is determined,  Dillon (1975;  Dillon and
Rigler, 1974a; 1974b; 1975)  has shown that when R(P) is calculated
and substituted into Equation 8, the equation can be rewritten as
            [P]  =(L(P) (l-R(P)))/z P                  (23)
     This equation attempts to consider the effects of phosphorus
retention, as well as flushing rate and phosphorus loading, on
the degree of fertility of a water body.   It should be noted that
the external loading, L(P), is in effect  lost as an independent
parameter since, by definition, L(P) (l-R(P)) is that part of the
external phosphorus loading which is lost through the outlet.
Thus, L(P)  (l-R(P)) can be defined as the average outflow con-
centration.  Therefore, in the strictest sense, Dillon's model
cannot be used for defining loading tolerances as long as there
is no valid model available for determining R(P).  Dillon
(Kirchner and Dillon, 1975) and Chapra (1975) have attempted to
derive an independent and valid model for R(P), as was mentioned
earlier.  The effect of mean depth as an independent parameter
is again partially lost since pw = l/tw = Q/V = Q/(A  • z), where
A  - surface area of water body.  Therefore, z pw = z  (Q/(A  • z))
=  Q/A. As indicated earlier, Q/A is the areal water loading.  Thus,
Equation 23 defines the steady state phosphorus concentration of
a  water body as directly proportional to the product of the phos-
phorus loading and outflow phosphorus loss  (i.e., "average out-
flow concentration"), and inversely proportional to the areal
                                 73

-------
water loading.  The areal water loading is equivalent to the
hydraulic loading, q  (i.e., q  = Q/A = Q/(V/z) = z(V/Q) =
z pw = Z/T^) .        s         s

     Inclusion of the factor (l-R(P), therefore, accounts for one
more source of variation in determining a water body's trophic
status.  Dillon (1975; Vollenweider and Dillon, 1974) prepared
a loading diagram upon which is plotted (L(P) (l-R(P) ) )/p versus z
(Figure 12).  Boundary lines representing phosphorus concentra-
tions of 0.01 mg/1 and 0.02 mg/1 (Sawyer, 1947; Sakamoto, 1966;
Dillon, 1975)  can be drawn on the diagram.  These boundary lines
correspond to Vollenweider ' s "permissible" and "excessive" bound-
ary conditions (Figures 7 and 8).  Water bodies below the 0.01
mg/1 phosphorus concentration line are considered oligotrophic and
those above the 0.02 mg/1 phosphorus concentration line are consid-
ered eutrophic.  The transition zone between the 0.01 and 0.02
mg/1 phosphorus concentration lines is considered the mesotrophic
zone .

     In Dillon's model, the trophic categorization of a water
body is based on measurement of the water body's phosphorus con-
centration, rather than its phosphorus loading.  This line of
reasoning is consistent with the view mentioned earlier that the
nutrient concentration, rather than nutrient loading, determines
a water body's degree of eutrophicat ion .

     Dillon's model has its quantitative  basis in the same simple
nutrient budget model as does Vollenweider ' s model (Vollenweider,
1975a).  In addition, it is a simple method for predicting phos-
phorus concentrations in water bodies .  If these concentrations
can, in turn, be related to water quality parameters that re-
flect a water body's trophic condition (e.g., chlorophyll con-
centrations, productivity,- Secchi depth,  etc.), then measurement
of phosphorus concentration becomes a very convenient way to
define or predict trophic status.  As mentioned earlier, Dillon
(1974a; Dillon and Rigler, 1974a) and other workers (Sakamoto,
1966; Jones and Bachmann , 1976) found such a correlation between
phosphorus concentration at spring overturn and predicted
average summer chlorophyll a concentration.

Larsen and Mercier Influent Phosphorus And Phosphorus Retention
                   ~~
     Larsen and Mercier (1976) shifted emphasis from phosphorus
loadings to average influent phosphorus concentrations as a
measure of trophic state.  They described the average phosphorus
concentration in a water body as a function of the relationship
between the mean influent phosphorus concentration and the water
body's ability to assimilate the influent phosphorus.  Their
model, like Dillon's model, was derived from the steady state
solution of a simple phosphorus mass balance model such as
                                74

-------
                  10
en
                       EUTROPHIC ZONE
            
-------
presented by Vollenweider (Equation 8) (1975a).  Recalling that
 0)
   = 1/T  and Z/T  = qs, Equation 23 can be rewritten as
               = L(P)  (l-R(P))
               =  (L(P)/q )  (l-R(P))
                        O
                     (l-R(P))
                                                       (24)
     where [P] = influent phosphorus concentration (mg/m )
                 = L(P)/q. and
                         o

        l-R(P) = fraction of phosphorus input not retained
                 by sediments.

This relationship is identical to that of Dillon (Equation 23)
since L(P)/z p^ = L(P)/qs = [P].  Thus Larsen and Mercier's
relationship relates the steady state phosphorus concentration
of a water body to the product of the influent phosphorus con-
centration and the fraction of the phosphorus input which is not
sedimented.

     Larsen and Mercier's (1976) relationship (Equation 24-) be-
tween water body steady state in-lake phosphorus concentration
and phosphorus retention is identical to that relationship im-
plicitly indicated earlier in Vollenweider's equation for deter-
mining the critical phosphorus loading for a water body, based
on its mean depth and hydraulic load (Equation 19).  According
to Vollenweider (1975) and Larsen and Mercier (1976), R(P) =
1/(1 + 4P  )•   Therefore, Equation 19 can be shown to be equiva-
lent to Equation 24 as follows:
L (P) =
 c
         from Equation 19
        10 -q (1
            s
                                         from Equation 24
Rearranging,

10 = (L (P)/q
                        /z7q~))
     = [P] (l-R(P))
Taking, for simplicity, Sawyer's
(1947) spring overturn critical
phosphorus concentration of 10
mg/m3 as [?]«,, and recalling
R(P) =!/(!
Since
               = [P], and
                                                             ' co
           then
                                  10 = [P] (i-d/a +
10 = [P]
The same results are obtained using either equation.

                                76

-------
     Larsen and Mercier (1976) prepared a phosphorus diagram to
show the relationship between a water body's influent phosphorus
concentration and its phosphorus retention capacity, as illus-
trated in Figure 13,,  Curves delineating trophic states can be
drawn on Larsen and Mercier's diagram in a manner analogous to
the method in which they have been plotted on the previous load-
ing diagrams.  Thus, this diagram can be used to determine the
reduction of a water body's influent phosphorus concentration
necessary to improve its trophic condition.  Since Larsen and
Mercier's diagram attempts to relate trophic state and in-lake
phosphorus concentrations, it can also be related to other para-
meters of water quality (e.g., chlorophyll concentrations, pro-
ductivity, Secchi depth, etc.).  For the same values of L(P),
pw  z, and R(P), the relative positions of lakes plotted on Dil-
lon's loading diagram (Figure 12) would be identical to those on
Larsen and Mercier's diagram (Figure 13) because both diagrams
estimate the same property, namely in-lake steady state phos-
phorus concentration, from the same variables.
                               77

-------
.-- 1000

X.
o>
O

h-
<
oi
o
z
O
o

V)
3
cr
o
x
a.
CO
O
UJ
ID
   100
20
    10
         EUTROPHIC  ZONE
    (FROM LARSEN 8. MERCIER, 1976)



        I    I     I     I
                                            'PERMISSIBLE
                              MESOTROPHIC
                                - ZONE
                                    OLIGOTROPHIC ZONE
                                I
I
I
I
               0.2         0.4        0.6        0.8



             PHOSPHORUS  RETENTION COEFFICIENT, R
                                                      1.0
 Figure 13.   Larsen and  Mercier Influent Phosphorus and
              Phosphorus  Retention Relationship.

-------
                            SECTION VI

             RESULTS OF THE INITIAL ANALYSIS OF THE US
                   OECD EUTROPHICATION STUDY DATA
     The overall approach utilized in the US OECD eutrophication
study involved giving each of the US investigators a small amount
of funds to develop a report covering the topics listed in
Appendix I.  Each investigator prepared a preliminary draft re-
port which was made available to all the other US OECD investi-
gators in the spring of 1974.  During the remainder of 1974 and
early 1975 each investigator revised his report so that it con-
formed to the form outlined in Appendix I.  The US EPA limited
each report to approximately 20 typewritten pages.  These reports
were submitted to the US EPA on or about July 1, 1975.  At that
time they were made available to the authors of this report for
examination.

     This section of this report involves a detailed examination
of the information provided on sampling, analytical and other
methodology used by the US OECD investigators to generate the
summary data sheet for their respective water bodies as presented
in Appendix II.  This section also examines the various methods
used by the US OECD investigators to estimate nutrient load-lake
or impoundment trophic response relationships.  Particular
attention was given to the nutrient loading estimates as they
are applied in the loading diagrams developed by Vollenweider
and others for establishing critical phosphorus loadings
and trophic state associations for lakes and impoundments.

SAMPLING AND MEASUREMENT METHODOLOGIES

     The US OECD water bodies were examined both for nutrient
flux and trophic response.  A water body's trophic response was
measured by a variety of physical, chemical and biological par-
ameters, as outlined in the Final Report Outline (Appendix I)
and summarized in the investigators' Summary Sheets (Appendix II).
The various response parameters deemed essential or desirable in
the OECD eutrophication study (Table 2) had been agreed upon
prior to the initiation of the study.  However,  most of the US
OECD water bodies had been extensively studied prior to initiation
of the US OECD eutrophication study.   In most cases the goals of the
                                79

-------
prior studies were often different from those of the US OECD
eutrophication study.  Also, the sampling and analytical method-
ologies employed in the earlier studies were often different from
those suggested and outlined by the OECD Water Management Sector
Group prior to initiation of the OECD eutrophication study.   A
summary of the analytical methodologies used by the US OECD  inves-
tigators in determining the major response parameters is presented
in Table 11, while the sampling methodologies are presented  on the
Summary Sheets (Appendix II).  Examination of Table 11 indicates
that while the US EPA (US EPA, 1971; 1973d; 1974b) and Standard
Methods (APHA ejt al. , 1971) served as the major sources of analyt-
ical methodology,"There was still a wide variety of methods  used
by the US OECD investigators to determine various parameters.  In
addition, the sampling regimes, including sampling depths, fre-
quencies, and durations, varied widely among investigators.   For
example, the "mean" value for a given parameter was biased both
by the period of sampling and the frequency with which the water
body was sampled.  Some water bodies were sampled at regular in-
tervals, while others were sampled only during the ice-free  period
or during a specific  month of the year.  Also, some water bodies
were sampled at many  depths while others were sampled only at a
few depths.  Any sampling and/or analytical errors were also in-
corporated into determination of the mean values .  The result of
these variations is that direct comparison of values between water
bodies is often not valid.  Standardization of all sampling method-
ologies and analytical procedures is necessary before such direct
comparison of trophic response parameters between US OECD water
bodies is valid.


NUTRIENT LOAD CALCULATION METHODOLOGIES

     The usefulness of the various Vollenweider phosphorus load-
ing relationships, as well as the relationships developed by
Dillon (1975) and Larsen and Mercier (1976), for establishing
critical phosphorus loading rates and trophic state associations
is dependent upon the accuracy of the water body's phosphorus
loading estimates.  Consequently, before reviewing the nutrient
load-trophic response relationships found in the US OECD eutrophi-
cation study, it is appropriate to review the various methods
used by the US OECD investigators to calculate the parameters nec-
essary for the various nutrient loading diagrams derived in
the previous section.

     A summary of the methods used to estimate the nutrient load-
ings to the US OECD water bodies is presented in Table 12.  Exami-
nation of this table  indicates a variety of different methods
were employed by the  US OECD investigators to estimate the nutri-
ent loadings.  An attempt was made to clarify and standardize
these various methodologies.  Such standardization is necessary
so that the loading estimates may be directly comparable between
water bodies in the US OECD eutrophication study.  However,  the

                                 80

-------
             Table lla.  ANALYTICAL PROCEDURES FOR MAJOR RESPONSE PARAMETERS EXAMINED
                         IN US OECD EUTROPHICAT30N STUDY - PHOSPHORUS AND NITROGEN
                         CONCENTRATIONS11

Water Body
Blackhawk
Brownie
Calhoun
Came lot -Sherwood
Complex
Dissolved
Phosphorus
Ascorbic Acid
Method (APHA
e_t al. , 1971)
-
-
Ascorbic Acid
Method (APHA
et al. , 1971)
Total
Phosphorus
Persulfate digestion
followed by Ascorbic
Acid Method (APHA
et al. , 1971)
-
-
Persulfate digestion
followed by Ascorbic
Acid Method (APHA
Ammonia Nitrate
Phenate Method
(APHA et al. ,
1971)
-
-
Phenate Method
(APHA et al. ,
1971)
Nitrite
-
-
-
"
Canadarago
Cayuga

Cedar

Dogfish
Murphy and Riley
Method (1962)
Not determined
Concentrated Sulfuric
Acid S Potassium Per-
sulfate digestion,
followed by Murphy 6
Riley Method (1962)
Potassium Persulfate
digestion, followed
by Phosphomolybdate/
Stannous Chloride
Reduction (APHA
et al., 1971)
Direct Nessleri-
zation (APHA et_
al. , 1971)
APHA et al.
(19711
Mullin and Riley AutoAnalyzer
Procedure (1955) or APHA et
if < 30 ug/1;    al.(19717~
AutoAnalyzer
if > 30 ug/1
Cadmium Reduc-
tion Method
(APHA et al_. ,
1971)
APHA et al.
(197lT~

-------
                      Table  lla  (continued).   ANALYTICAL  PROCEDURES FOR MAJOR RESPONSE PARAMETERS
                                 EXAMINED IN  US OECD  EUTFOPHICATION  STUDY - PHOSPHORUS AND NITROGEN
                                 CONCENTRATIONS3  '

Water Body
Dutch Hollow
Dissolved
Phosphorus
Ascorbic Acid
Method (APHA
et a^. , 1971)
Total
Phosphorus
Persulfate digestion,
followed by Ascorbic
Acid Method (APHA
et 'al. , 1971)
Ammonia Nitrate
Phenate Method
(APHA et al. ,
1971)
Nitrite
-
oo
r-o
George

Harriet

Isles

Kerr Reservoir
         Lamb
Automated Phos-
phomolybdate/
Stannous Chlo-
ride Reduction
(APHA et^ al. ,
1971X Ascorbic
Acid Reduction
Method used af-
ter July, 1975
(APHA et_ aJL. ,
1971)

Not determined
                                              Potassium  PersulfateS  Automated  Pheno-  Automated Hydrazine Reduction
                                              Sulfuric Acid diges-
                                              tion,  followed by
                                              Ascorbic Acid reduc-
                                              tion Method  (APHA
                                              et al., 1971)
                                                           late Method with
                                                           Technicon Auto-
                                                           Analyzer I (US
                                                           EPA, 1971)
                  with Technicon AutoAnalyzer I
                  (US EPA, 1971) from 1966-
                  1975; Cu/Cd Reduction  (US
                  EPA,1974b) after  July,  1975
                                    Potassium Persulfate
                                    digestion, followed
                                    by Phosphomolybdate/
                                    Stannous Chloride
                                    Reduction (APHA
                                    et al.,  1971)
APHA et aJU
(1971F"
Cadmium Reduc-
tion Method
(APHA et_ al.
1971)
                                                                                                       APHA et al.
                                                                                                       (1971"5

-------
             Table lla (continued).  ANALYTICAL PROCEDURES FOR MAJOR RESPONSE  PARAMETERS
                       EXAMINED IN US OECD EUTROPHICATION STUDY - PHOSPHORUS AND  NITROGEN
                       CONCENTRATIONSa

Dissolved Total
Water Body Phosphorus Phosphorus
Meander Not determined Potassium Persulfate
digestion, followed
by Phosphomolybdate/
Stannous Chloride
Reduction (APHA
et al. , 1971)
Mendota Analytical procedures outlined
Michigan Analytical procedures outlined
00
MinT~lf^1~OnK^ PVir-itrriV-n-imnTwHH^-l-tj/ P/^v^c-iilfja-f-cs Hirrdo-f-T^T-i
Ammonia
APHA et al.
(1971F"




in Lee (1966)
in Rousar (1973)

Mnf dp tprrrn npd
Nitrate Nitrite
Cadmium Reduc- APHA et al .
tion Method (197lT~
APHA et al .
1971)





Not determined Not deter-
Potomac Estuary

Redstone
Sallie

Sammamish
Phosph
Ascorbic Acid
Reduction (APHA
e_t a_l. ,  1971 )


US EPA 91971)

Ascorbic Acid
Method (APHA
et al. , 1971)
                  followed  by  Phospho-
                  molybdate/Ascorbic Acid
                  Reduction (APHA et_ al. ,
                  1971)

                  US EPA (1971)

                  Persulfate digestion,
                  followed  by Ascorbic
                  Acid Method (APHA
                  et al.,  1971)
US EPA (1971)

Phenate Method
(APHA et aJ.,
1971)
                                                                             US  EPA (1971)
                                                                                              mined
US EPA 0971)
        "As outlined in APHA et aJU , 1971"

Molybdate Complexing Reaction
(Strickland and Parsons, 1968)
                 Cadmium-Copper
                 Column  (Strick-
                 land and Par-
                 sons , 1968)

-------
                       Table lla (continued).   ANALYTICAL  PROCEDURES FOR MAJOR RESPONSE PARAMETERS
                                 EXAMINED IN US OECD  EUTROPHICATION STUDY - PHOSPHORUS AND NITROGEN
                                 CONCENTRATIONS3
            Water Body
  Dissolved
  Phosphorus
                      Total
                      Phosphorus
   Ammonia
Nitrate
Nitrite
cx>
-P
          Shagawa
          Stewart
          Tahoe

          East Twin
          West  Twin
          Twin  Valley
Murphy-Riley As-  Persulfate digestion,
corbie Acid Meth- followed by Murphy-
od (US EPA, 1971) Riley Ascorbic Acid
                  Method (US EPA, 1971)
Ascorbic Acid
Method (APHA
et al. , 1971)
                  Persulfate digestion,
                  followed by Ascorbic
                  Acid Method (APHA
                  et al. ,  1971)
Phosphomolybdate/ Persulfate Sulfuric
                            Ascorbic Acid
                            Reduction (APHA
                            et al.,  1971)
                  Acid digestion,
                  followed by Phos-
                  phomolybdate /Ascorbic
                  Acid Reduction (APHA
                  et al. ,  1971)
Phosphomolybdate/ Persulfate Sulfuric
                            Ascorbic Acid
                            Reduction (APHA
                            et  al. ,  1971)
Ascorbic Acid
Method (APHA
et al. , 1971)
                  Acid digestion,
                  followed by Phos-
                  phomolybdate /As corbie
                  Acid Reduction (APHA
                  et al_. ,  1971)
                  Persulfate digestion,
                  followed by Ascorbic
                  Acid Method (APHA
                  et al. ,  1971)
Automated Indo-   Automated Cadmium Reduction
phenol Blue Meth- followed by Diazotization
od (US EPA, 1971) (US EPA, 1971)

Phenate Method
(APHA et al.,
1971)
                                         Direct Nessleri-  Cadmium Reduc-
                                         zation (APHA et^   tion (APHA et
                                         al. , 1971)        aJL. , 1971)
                                         Direct Nessleri-
                                         zation (APHA et
                                         al. , 1971)
Phenate Method
(APHA et al.,
1971)
                  Cadmium Reduc-
                  tion (APHA et_
                  al. , 1971)

-------
                      Table  ]la  (continued).  ANALYTICAL PROCEDURES  FOR  MAJOR  RESPONSE  PARAMETERS
                                 EXAMINED IN US OECD EUTROPHICATION STUDY -  PHOSPHORUS AND NITROGEN
                                 CONCENTRATIONS3
            Water Body
                    Dissolved
                    Phosphorus
               Total
               Phosphorus
                                                                        Ammonia
                     Nitrate
                    Nitrite
oo
en
         Virginia
Waldo

Washington
         Weir
         Wingra
                  Ascorbic Acid
                  Method (APHA
                  et al., 1971)
                           US EPA  (1973d)
           Persulfate digestion,
           followed by Ascorbic
           Acid Method (APHA
           ejt al. ,  1971)

           US EPA (1973d )
Phenate Method
(APHA et al.,
1971)
US EPA  (1973d)   US EPA  (1973d)    US  EPA (1973d)
(Note:   Many different methods have been used over the years by different
investigators.   The methods reported here are those of more recent years'
studies (Edmondson, 1975b))
                           Phosphomolybdate/ Perchloric Acid diges- Direct Nessleri-  "Strychnidine
                           Stannous Chloride tion, followed by      zation (APHA  et   Method  until
                           Reduction  (APHA   Phosphomolybdate/      al.,  1971)
                           e_t^ al_. , 1971)     Stannous Chloride
                                             Reduction (APHA
                                             ejt aJU , 1971)
                                                                            August, 1967,
                                                                            then Brucine
                                                                            Method  (APHA
                                                                            et al. , 1971)
                           US EPA  (1971)
                  Murphy and Riley
                  Method (1962)
                                    US EPA (1971)
           Persulfate  digestion;
           followed by Murphy
           and Riley Method
           (1962)
Automated Al-
kaline Phenol
Procedure (US
EPA, 1971)

Alkaline phenol
procedure
adopted for
AutoAnalyzer
Automated Hydra- Not deter-
zine Reduction   mined
Procedure,
Henrikaen (1965)

Initially Hydra- Not deter-
zine Reduction   mined
Procedure. Later
the Brucine Method
of Kahn 6 Brezenski
(1967)
         aAs indicated by the US OECD investigators.
          Dash (-) indicates no data available.

-------
                         Table  lib.   ANALYTICAL PROCEDURES FOR MAJOR RESPONSE  PARAMETERS EXAMINED  IN
                                      US OECD EUTROPHICATION STUDY - TRANSPARENCY, PRIMARY  PRODUCTIVITY
                                      AND CHLOROPHYLL a AND DISSOLVED OXYGEN CONCENTRATIONS3
              Water Body
                   Water
                   Transparency
       Dissolved
       Oxygen
     Chlorophyll a
   Primary
   Produc civity
            Blackhawk
                   Secchi disc
co
en
Brownie

Calhoun

Camelot-Sherwood   Secchi disc
Complex

Canadarago         30 cm white
                   Secchi disc
            Cayuga
            Cedar
            Dogfish            Secchi disc
YSI Model 54 D.O.
Meter
YSI Model 54 D.O.
Meter
Weston and Stack D.O.
Meter; some surveys
made using Winkler
Method with Azide
Modification (APHA
et al., 1971)
            Dutch Hollow
                   Secchi disc
YSI Model 54 D.O.
Meter
Strickland and Parsons
(1965)
Strickland and Parsons
(1965)
Strickland and Parsons
(1965) See Hetling et_
al. (1975) for varia-
tions between 1968 and
subsequent determina-
tions
Strickland and Parsons
(1968) until May, 1972;
Turner Fluorometer after
May, 1972
Strickland and Parsons
(1965)
Not determined
Not determined

Method developed
by principal in-
vestigators (see
Hetling et a^.,
1975 for~details)

Not determined

Not determined

Not determined
                                                                                                  Not determined

-------
             Table lib (continued).  ANALYTICAL PROCEDURES FOR MAJOR RESPONSE PARAMETERS
                       EXAMINED IN US OECD EUTROPHICATION STUDY - TRANSPARENCY, PRIMARY
                       PRODUCTIVITY AND CHLOROPHYLL a AND DISSOLVED OXYGEN CONCENTRATIONS'5
  Water Body
Water
Transparency
Dissolved
Oxygen
Chlorophyll a
Primary
Productivity
George


Harriet
Isles
Kerr Reservoir
Lamb
Meander
Mendota

Michigan

Minnetonka
                                        Not determined
 8  inch diameter
 White Secchi
 disc
                   Secchi disc
                   Secchi disc
                                    Hydrolab Surveyor £
                                    Azide Modification
                                    of Winkler Method
                 Turner Fluorometer
                                                           Strickland and Parsons
                                                           (1968) until May, 1972;
                                                           Turner Fluorometer after
                                                           May, 1972
                                        Strickland and Parsons
                                        (1968) until May, 1972;
                                        Turner Fluorometer after
                                        May, 1972

     Analytical procedures outlined in Lee (1966)

     Analytical procedures outlined in Rousar (1973)
Secchi disc and
attenuation
coefficients
                 Strickland and Parsons
                 (1968)
                      1 14
                        C uptake
                      (Steeman-Nielsen,
                      1952)
                      Not determined

                      Not determined

                      Oxygen Production
                      under standard
                      laboratory con-
                      ditions (i.e. ,
                      24°C, 400 foot
                      candles)

                      Not determined
                                                                                      Not determined
                                            Not determined

                                            Not determined

-------
             Table  lib  (continued).  ANALYTICAL PROCEDURES FOR MAJOR RESPONSE  PARAMETERS
                        EXAMINED IN US OECD EUTROPHICATION STUDY - TRANSPARENCY,  PRIMARY
                        PRODUCTIVITY AND CHLOROPHYLL a AND DISSOLVED OXYGEN  CONCENTRATIONS3

Water Body
Potomac
Estuary
Redstone
Sallie
Sammamish
Shagawa
CO
CO
Water Dissolved
Transparency Oxygen
Secchi disc Winkler Method; Azide
Modification (APHA et al
Secchi disc YSI Model 54 D.O.
Meter
"As outlined in APHA et al . , 1971"
Secchi disc Winkler Method; Azide
Modification (APHA
et al. , 1971)
Secchi disc Winkler Method; Azide
Modification (EPA,
1971)
Chlorophyll a
90% Acetone extraction
., 1971)
Strickland and Parsons
(1965)
90% Acetone extraction
(Strickland and Parsons,
1968)
90% Acetone extraction
(UNESCO, 1966)
Primary
Productivity
Not determined
Not determined
Not determined
14
C uptake
(Strickland and
Parsons, 1968)
Oxygen production;
light and dark
bottle procedure
Stewart

Tahoe

East Twin
West Twin
 Secchi  disc
 20 cm  dia.
 Secchi disc;
 alternating
 black  g white
 quadrants

•20 cm  dia.
 Secchi disc ;
 alternating
 black  £ white
 quadrants
                YSI Model 54 D.O.
                Meter
Strickland and Parsons
(1965)
Strickland and Parsons
(1968), with trichromatic
equations (APHA et al.,
1971)


Strickland and Parsons
(1968), with trichromatic
equations (APHA et_ al . ,
1971)
Not determined
pH method in light
and dark bottles
after 4 hours of
incubation


pH method in light
and dark bottles
after 4 hours of
incubation

-------
                             Jit' (continued).   ANALYTICAL PROCEDURES FOR MAJOR RESPONSE  PARAMETERS
                                 EXAMINED IN  US OECD EUTROPH1CAT TON STUDY - TRANSPARENCY,  PRIMARY
                                 PRODUCTIVITY AND CHLOROPHYLL a AND DISSOLVED OXYGEN  CONCENTRATIONS'3
CO
CD

Water Body
Twin Valley

Virginia

Waldo
Washington
Water
Transparency
Secchi disc

Secchi disc

20 cm white
Secchi disc
(Note : Many
Dissolved
Oxygen
YSI Model 5U D.O.
Meter
YST Model 5't D.O.
Meter
-
different methods have
Chlorophyll a
Strickland and Parsons
(1965)
Strickland and Parsons
(1965)
"Strickland and Parsons
been used over the years by-
Primary
Productivity
Not determined

Not determined

" C uptake
different
                            investigators.   The  methods reported here are those of more  recent  years'
                            studies  (Edmondson,  1975b).

                            Secchi disc                -              Strickland and Parsons
                                                                     (1968) Prior to  1968,
                                                                     used acetone extraction
                                                                     and Klett colorimeter
Oxygen production
in light and dark
bottles.14C uptake
done for several
years
Weir
Wingra
Secchi disc
Secchi disc YSI D.O. Meter
Trichromatic Method
(US EPA, 1973d )
Not determined
14C uptake (APHA
et aJL. , 1971)
See Huff et al .
(1972)
          As indicated by the US OECD investigators.
          Dash (-) indicates no data available.

-------
                               Table 32. SUMMARY Of METHODS UCFD TO CALCULATE
                                         NUTRIENT LOADINGS TOR US OECD WATER BODIES
              Water Body
Nutrient Sources Considered by
US OECD Investigator1 in Mutrient
Loading Estimates
General Methodology Indicated by
Investigator to Determine the
Nutrient Loading to Water Body
              Blackhawk,  Camelot-
              Sherwood, Cox Hollow,
              Dutch Hollow, Redstone,
              Stewart, Twin Valley
              and Virginia
    A)  Phosphorus Loading:
         1)  Base Flow
         7)  Woodland
         3)  Rural Runoff
         U)  Urban Runoff
         5)  Manured Lands
         6)  Precipitation
         7)  Dry Fallout
         8)  Domestic Wastewaters
         9)  Septic Tanks
        10)  Drained Marshes
        11)  Groundwater
  •Phosphorus loadings estimated
   from watershed land usage
   phosphorus export coefficients
   derived for the Lake Mendota
   (Wisconsin) watershed and
   presented in Sonzogni and
   Lee (1974).
CD
O
                                          B)  Nitrogen  Loading:
                                        -Same sources and methods as
                                         for phosphorus loadings.
                                         Watershed nitrogen export co-
                                         efficients were used to cal-
                                         culate the nitrogen loadings.
              Brownie, Calhoun,
              Cedar, Harriet and
              Isles
    A)  Phosphorus Loading:
         1)  Waste Discharges
             (includes city water
             and air conditioning
             water)
         2)  Land Runoff (via storm
             drain and direct)
         3)  Estimated Precipitation
         4)  Estimated Groundwater
             Input

    B)  Nitrogen Loading:
                                                                              -No information available.
                                                                              -Not Determined.

-------
           Table 12(continued).   SUMMARY OF METHODS  USED TO CALCULATE
                            NUTRIENT  LOADINGS  FOR US  OECD WATER  BODIES
Water Body
Nutrient Sources Considered by
US OECD Investigator in Nutrient
Loading Estimates
General Methodology Indicated by
Investigator to Determine the
Nutrient Loading to Water Body
Canadarago
    A)  Phosphorus Loading:
        T~5  Wastewater Discharges
                                2)   Septic  Tanks
                                3)   Gaged Tributaries
  -Estimates were made by direct
   measurement of the primary
   wastewater treatment plant to
   Ocquionos Creek (one of maior
   tributaries to lake), and the
   difference between upstream and
   and downstream samples from
   Ocquionos Creek, and calculations
   from published per capita contri-
   butions .

  - Estimate made by calculations
   involving total population of
   lakeside residences, lakeside
   residence population having
   septic  tank failures, average
   residence time of lakeshore
   facilities and per capita phos-
   phorus  input value of 2.9 g
   P/capita/day.  It was assumed
   any phosphorus entering a septic
   tank leaching field was re-
   tained  in the field, unless the
   tank discharged directly into the
   lake.
  -Estimated as product of measured
   daily flows and phosphorus con-
   centrations .

-------
           Table 12 (continued).   SUMMARY  OF  METHODS  USED TO  CALCULATE
                            NUTRIENT LOADINGS  FOR  US OECD WATER BODIES
Water Body
Nutrient Sources Considered by
US OECD Investigator in Nutrient
Loading Estimates
General Methodology Indicated by
Investigator to Determine the
Nutrient Loading to Water Body
Canadarago
(continued)
        4)  Non-gaged Tribu-
            taries
                                5)   Rainfall  and  Dry
                                    Fallout
                                6)   Groundwater

                            B)  Nitrogen  Loading:
Cayuga
     A)   Phosphorus Loading:

         1)  Waste Discharge
  -Assumed runoff for  non-gaged
   area was equal to the average
   of the area drained by the
   gaged  tributaries, not count-
   ing the wastewater treatment
   plant effluents.

  -Estimated from literature
   values; mainly Weibel (1969).

  -Considered negligible.

  -Same sources and methods as
   for phosphorus loadings.  For
   the septic tank nitrogen load-
   ings, 10.3 g N/capita/day
   was used in the calculations.
   It was assumed that no nitro-
   gen was retained in the septic
   tank leaching fields; there-
   fore, it was assumed the entire
   lakeshore population with
   septic tanks contributed nitro-
   gen to the lake.  Nitrogen fix-
   ation was not considered in the
   nitrogen loading estimates.
                                                                -Determined using estimates of
                                                                 per capita discharge of phos-
                                                                 phorus to tributaries and
                                                                 phosphorus in waste discharged
                                                                 directly to lake.

-------
                     Table I? (continued).  SUMMARY Of METHODS USED TO CALCULATE
                                      NUTPIENT LOADINGS EOR US OECD WATER BODIES
          Water Body
Nutrient Sources Considered by
US OECD Investigator in Nutrient
Loading Estimates
General Methodology Indicated by
Investigator to Determine the
Nutrient Loading to Water Body
          Cayuga
          (continued)
                                         2)  Land Runoff



                                         3)  Precipitation


                                         t)  Groundwater
                                        -Estimated per capita discharge
                                         of phosphorus to tributaries minus
                                         phosphorus in waste discharged
                                         directly to lake.

                                        -Phosphorus in precipitation
                                         monitored in one year  study.

                                        -Information not available.
to
CO
     NOTE:  1)  Total phosphorus  input and molybdate reactive
               (unfiItered) phosphorus  input taken from Likens
               (1972b; 1974a;  1974b).

           2)  Phosphorus  in precipitation and  in 25 tribu-
               taries  (draining  78% of  watershed) was moni-
               tored in a  one  year study.
           3)  "Biologically reactive phosphorus" determined
               using nutrient  export coefficients; forest  =_
               8.3 mg/m /yr; agricultural/rural  = 13.2 mg/m /yr;
               urban = 100 mg/m  /yr.
                                     B)  Nitrogen Loading:
                                                                         -Same general methods as for
                                                                          phosphorus loadings.
                                                                         -t.U4 kg N/yr used as per capita
                                                                          N discharge.  (Olsson, Kargren and
                                                                          Tullander, 1968) .
                                                                         -Sewage treatment efficiency (all
                                                                          types of disposal systems) of 50
                                                                          percent for N removal was assumed.

-------
                      Table 1 2 (con tinned).   SUMMARY  OF METHODS  USED  TO  CALCULATE
                                       NUTRIENT  LOADINGS  FOR  US OCCF) WATER BODIES
            Water Body
Nutrient Sources Considered by
US OECD Investigator in Nutrient
Loading Estimates
General Methodology Indicated  by
Investigator to Determine  the
Nutrient Loading to Water  Body
           Dogfish,  Lamb  and
           Meander
CO
-p
           George
    A)  Phosphorus Loadings :
        1)  Atmosphere
            (wet and dry)
        2)  SurFace Flow
            (sheet flow +
            Flow through
            soils)
        3)  Tributary Flow
                                            4)  Groundwater
                                                                            -Determined by measurement of
                                                                             samples  of water collectors
                                                                             placed throughout drainage
                                                                             basin.   Snow samples also
                                                                             analyzed.

                                                                            -Measured at two-week inter-
                                                                             vals  during April-October.
  -Measured at two-week inter-
   vals during April-October.
   Tributaries monitored by
   grab sample, and flows de-
   termined manually on day of
   sampling.
  -Assumed zero.
                                               Details of 1972 nutrient  budgets  available  in
                                               Wright  (1974) and  Bradbury  e_t_ al.  (1974)
    B)  Nitrogen Loading:

    A)  Phosphorus Loading:
        1)  Runoff
        2)  Precipitation
        3)  Sewage Plant Effluents
        4)  Septic Tank Effluents
        5)  Lawn Fertilizer
                                                                            -Not  determined.
                                                                           -Taken  from  Gibble  (1974).
                                                                             (Precipitation  based  on  "normal
                                                                             precipitation of basin").
                                       B)  Nitrogen Loading:
                                        -Not Determined

-------
                    Table 12 (continued).   SUMMARY OF METHODS USED TO  CALCULATE
                                     NUTRIENT LOADINGS FOR US OECD WATER BODIES
         Water  Body
Nutrient Sources Considered by
US OECD Investigator in Nutrient
Loading Estimates
General Methodology Indicated by
Investigator to Determine the
Nutrient Loading to Water Body
         Kerr Reservoir
    A)  Phosphorus Loading:
        1)  Point Sources
CD
en
        2)  Gaged Tributary
            Sources
        3)  Non-gaged Tributary
            Sources
                                         U)  Rainfall
                                                                         -Virginia data assembled from
                                                                          tabulation prepared by Hayes,
                                                                          Seay and Mattern for the
                                                                          Roanoke River Basin Study and
                                                                          provided by the Wilmington
                                                                          District, US Army Corps of
                                                                          Engineers.  North Carolina
                                                                          data is from Division of En-
                                                                          vironmental Management, De-
                                                                          partment of Economic and
                                                                          Natural Resources.

                                                                         -Information not available.
  -Equal to total discharge minus
   gaged stream discharge.  Phos-
   phorus and nitrogen concentration
   estimates from five non-polluted
   feeder streams were applied to
   the volume to obtain input from
   non-gaged sources.

  -Taken from nutrient coefficient
   data of Uttormark et al.,  (1974)
   and Gambell and Fisher  (1966).
   Also, total phosphorus was deter-
   mined on rainfall samples  collect-
   ed at Chapel Hill, North Carolina
   on April 13 and April 25,  1972.
                                         5)  Groundwater Seepage
                                        -Considered  insignificant.

-------
                     Table 12 (continued).   SUMMARY  OF METHODS USED TO  CALCULATE
                                      NUTRIENT  LOADINGS FOR US  OECD WATER  BODIES
          Water Body
Nutrient Sources Considered by
US OECD Investigator in Nutrient
Loading Estimates
General Methodology Indicated by
Investigator to Determine the
Nutrient Loading to Water Body
          Kerr Reservoir
          (continued)
          Hendota
to
CD
          Michigan
            Open Waters
    B)  Nitrogen Loading:
    A)  Phosphorus Loading:

        1)  Wastewater Discharges
        2)  Urban Runoff
        3)  Rural Runoff
        4)  Precipitation
        5)  Dry Fallout
        6)  Groundwater Seepage
        7)  Base Flow
        8)  Marsh Drainage

    B)  Nitrogen Loading:
     A)   Phosphorus Loading:
  -Same sources and methods
   as for phosphorus loadings.
   In addition, dry fallout and
   nitrogen fixation loadings
   considered insignificant.
                                                                           All  nutrient loading data
                                                                           taken from Sonzogni and
                                                                           Lee  (1974).
  -Same sources and methods as
   for phosphorus loadings.  In
   addition, nitrogen fixation
   was included in the nitrogen
   loading estimate.

  -1971 phosphorus loadings were
   taken from Lee (1974a)
   and included phosphorus loadings
   from:
   ])  direct wastewater,
   2)  indirect wastewater,
   3)  erosion and other diffuse
       sources,
   4)  combined sewer overflow, and
   5)  precipitation and dry fall-
       out onto lake surface.

-------
             Water Body
                       Nutrient Sources Considered  by
                       US OECD Investigator  in  Nutrient
                       Loading Estimates
                                  General Methodology Indicated by
                                  Investigator to Determine the
                                  Nutrient Loading to Water Body
             Michigan
               (Open Waters)
               (cont inued)
                                                                -1974 phosphorus loadings were
                                                                 taken from Lee (197Ua).
to
-J
Michigan

  Nearshore Waters

  Offshore Waters

Lower La. •* Minnetonka
                                         B)   Nitrogen Loading
--Nutrient Loadings Not Determined

--Information Not Available

A)  Phosphorus Loading:

    1)  Sewage Effluents
    2)  Tributary Streams
    3)  Overland Runoff
    4)  Rainfall on Lake
    5)  Septic Tank Drainage
                                                                -Taken from Bartsch (1968)
                                                                             -All nutrient loading data taken
                                                                              from compilations made by
                                                                              Harza Engineering Company ("A
                                                                              Program Eor Preserving The
                                                                              Quality Of Lake Minnetonka").
                                                                              State of Minnesota Pollution
                                                                              Control Agency,  Minneapolis,
                                                                              Minnesota.  1971. (Megard, 1975).

                                                                             -Overland runoff was estimated
                                                                              as 130 Ibs/mi /yr for2rural
                                                                              runoff and 510 Ibs/mi /yr for
                                                                              urban runoff.

                                                                             -Phosphorus concentration in   .
                                                                              rainfall assumed to be 20 mg/m .
                                         B)  Nitrogen Loading:
                                                                -Not Determined.

-------
                            Table J2 (continued).   SUMMARY  OF  I'L'TIIODG  USED  TO  CALCULATE;
                                             NUTRTFNT  LOADINGS  FOR  US OFCD WATFR  BODIES
                  Water Body
Nutrient Sources Considered by
US OECD Investigator in Nutrient
Loading Estimates
General Methodology Indicated by
Investigator to Determine the
Nutrient Loading to V/ater Body
                  Potomac Estuary
CJD
oo
                  Sallie
    A)  Phosphorus Loading:

        1)  Upper Basin Runoff
            (Note:  Upper basin
            runoff includes both
            land runoff and waste-
            water discharges in
            upper basin)

        2)  Estuarine Wastewater
            Discharges

        3)  Precipitation
        U)  Groundwater

     B)  Nitrogen Loading:
    A)  Phosphorus Loading:
        1)  Waste Discharge
                                                                                  -Based on  two  years  of  weekly
                                                                                   sampling  of upper basin
                                                                                   runoff.
                                                                                  -Based  on  two  years  of  weekly
                                                                                   samplings of  point  sources.

                                                                                  -Considered insignificant.   Dry
                                                                                   fallout not considered in
                                                                                   phosphorus loading  estimate.

                                                                                  -Same sources  and  methods as
                                                                                   for phosphorus  loadings.   Ni-
                                                                                   trogen fixation and dry fall-
                                                                                   out not considered  in  nitrogen
                                                                                   loading estimate.
                                                                                  -Waste  discharged  from City  of
                                                                                   Detroit  Lakes  into  Pelican
                                                                                   River  which  discharges into
                                                                                   lake.  Concentrations of  phos-
                                                                                   phorus in  ditch to  river  was
                                                                                   monitored  and  converted to
                                                                                   weight.

-------
                     Table 12 (continued).  SUMMARY OF METHODS USED TO CALCULATE
                                     NUTRIENT LOADINGS FOR US OECD WATER BODIES
           Water Body
Nutrient Sources Considered by
US OECD Investigator in Nutrient
Loading Estimates
General Methodology Indicated by
Investigator to Determine the
Nutrient Loading to Water Body
           Sallie
           (continued)
co
CO
           Sammamish
        2)  Land Runoff
                                          3)  Precipitation
                                          4)  Groundwater
    B)  Nitrogen Loading:


    A)  Phosphorus Loading:

        1)  Waste Discharge

        2)  Land Runoff
  -Estimated as total in Pelican
   River minus waste load total
   in other surface inlets.

  -Phosphorus concentration in pre-
   cipitation was monitored and
   converted to weight as product
   of lake area and total precipi-
   tation .

  -Collected with investigator-
   designed sampler as it entered
   lake.  Phosphorus weight was
   calculated for discharge in-
   crease over surface inflow.

  -Same sources and methods for
   phosphorus loadings.
                                                                          -Several independent methods.

                                                                          -Equal to total phosphorus load-
                                                                           ing plus precipitation phosphorus
                                                                           loading.

                                                                          -Total phosphorus loading equal
                                                                           to sum of measurement of 13
                                                                           streams and pipes entering lake
                                                                           plus waste contributions by
                                                                           several independent methods.

-------
                   Table 12 (continued).   SUMMARY  OF METHODS USED TO CALCULATE
                                    NUTRIENT LOADINGS  FOR US  OECD  WATER  BODIES
         Water Body
Nutrient Sources Considered by
US OECD Investigator in Nutrient
Loading Estimates
General Methodology Indicated by
Investigator to Determine the
Nutrient Loading to Water Body
         Sammamish
         (continued)
         3)  Precipitation
                                         U)   Groundwater
                                     B)   Nitrogen  Loading:
o
o
         Shagawa
     A)   Phosphorus Loading:

         1)  Waste Discharges
  -Atmospheric phosphorus input
   to lake surface determined
   from limited rainwater analysis
   during 1971 water year.

  -Determined as insignificant
   because water balance was
   explainable from consideration
   of surface inputs and outputs.

  -Same sources and methods for
   phosphorus.  In addition, dry
   fallout nitrogen input was not
   considered in nutrient loading
   estimate.  Nitrogen fixation
   was considered insignificant.
                                                                         -In  1971 and earlier years, waste
                                                                         discharges determined  from single
                                                                         daily grab samples and  some  four
                                                                         and six hour-nonweighted composites
                                                                         obtained.  In  1972, waste dis-
                                                                         charges computed phosphorus  con-
                                                                         centrations in the wastewater ob-
                                                                         tained from 24 hour flow-weighted
                                                                         composite samples.  Loadings were
                                                                         the product of composite concen-
                                                                         trations and the total  daily flows.

-------
          Table 1? (continues!) .   SUMMARY OF" METHODS USED TO  CALCULATE
                           NUTRIENT LOADINGS FOR US OECD WATER BOD FES
Water Body
Nutrient Sources Considered by
US OECD Investigator in Nutrient
Loading Estimates
General Methodology Indicated by
Investigator to Determine the
Nutrient Loading to Water Body
Shagawa
(continued)
        ?)  Land Runoff
            Tribu taries
                                3)  Precipitation
                                    Other (= direct
                                    runoff + excess
                                    drinking water)
                            B)  Nitrogen Loading:
  -Weekly, nonflow-weighted phos-
   phorus concentrations were in-
   tegrated to obtain daily values
   for creeks.  Daily loads were
   product of concentration and
   daily flow.  Prior to 1972, month-
   ly loading was product of monthly
   mean phosphorus concentration and
   total stream flow for month. Non-
   gaged tributaries estimated as
   ratio of non-gaged to gaged area,
   and multiplying the loading by the
   factor.
  -Estimated using average  phospho-
   rusconcentration collected at Ely,
   Minnesota, and multiplying by the
   monthly precipitation falling on
   the lake.
  -An average load/unit area/month
   was calculated based on the load/
   unit area/month for the gaged
   basins.

  -Same sources and methods as for
   phosphorus loadings.  Nitrogen
   inputs from wastewater treat-
   ment plants were calculated in
   a manner similar to that used
   to determine the phosphorus loadings.

-------
                     Table ]2 (continued).  SUMMARY OF METHODS USED TO CALCULATE
                                      NUTRIENT LOADINGS EOR US OECD WATER BODIES
            Water Body
Nutrient Sources Considered by
US OECD Investigator in Nutrient
Loading Estimates
General Methodology Indicated by
Investigator to Determine the
Nutrient Loading "to Water Body
           Tahoe
    A)  Phosphorus Loading:
    NOTE:  According to state and federal regulations
           no wastewater is supposed to be discharged
           within the drainage basin.
                                           1)
            Land Runoff
            (1969 data)
o
rv>
                                           2)  Precipitation
  -Total monthly discharge of
   nine major tributaries cal-
   culated from daily USGS
   flow measurements.   Total
   monthly discharge of other
   54 creeks and tributaries esti-
   mated as in McGauhey et al.(1963).
   Phosphorus concentration data col-
   lected on nine major tributaries
   by the Tahoe Research Group of
   the Univ. of California at Davis,
   the California-Nevada Federal
   Joint Water Quality Investigation,
   Lake Tahoe Area Council and the
   Water Resources Information Series
   of the State of Nevada.  Total
   phosphorus mass calculated as
   product of total flow and mean
   concentration.
  -Only traces of phosphorus were
   assumed to be present in rain-
   fall.

-------
                   Table 12 (continued).  SUMMARY OF METHODS USED TO CALCULATE
                                    NUTRIENT LOADINGS FOR US OECD WATER BODIES
         Water  Body
Nutrient Sources Considered by
US OECD Investigator in Nutrient
Loading Estimates
General Methodology Indicated by
Investigator to Determine the
Nutrient Loading to Water Body
         Tahoe
         (continued)
         Twin Lakes
o
CO
         3)  Groundwater

     B)   Nitrogen Loading:
    A)   Phosphorus  Loading:
         1)  Waste Discharges

         2)  Land Runoff
            ("sheet" runoff)
                                         3)  Precipitation
  -Assumed insignificant input.

  -The same sources and methods
   as for phosphorus loading.
   In+addition, the average
   NHl(-N and NO^-N were measured
   in the precipitation to esti-
   mate the total nitrogen input
   from rainfall.
  -Assumed zero.
  -Computed from lake  level  in-
   creases, as recorded by limno-
   graphs, in excess of that
   from direct precipitation and
   stream inflows.

  -Measured with a recording
   Leupold-Stevens type Q6
   weighing bucket located at West
   Twin Lake.  Rain and snow sam-
   ples (which included dry  fall-
   out) were collected at Kent
   State University, Kent, Ohio,
   for nutrient analysis.

-------
                     Table 12 (continued).   SUMMARY OF METHODS  USED TO  CALCULATE
                                      NUTRIENT LOADINGS  FOR US OECD WATER BODIES
           Water Body
Nutrient Sources Considered by
US OECD Investigator in Nutrient
Loading Estimates
           Twin Lakes
           (continued)
        4)  Groundwater
H
CD
-P
                                           5)   Surface  Streams
           Waldo
                                       B)  Nitrogen Loading:
    A)   Phosphorus Loading:

    NOTE:  Dry fallout was not
           considered in phos-
           phorus loading esti-
           mate.  Marsh drain-
           age considered in-
           significant .
General Methodology Indicated by
Investigator to Determine the
Nutrient Loading to Water Body
  -Twenty-eight shallow wells were
   installed around lake perimeter
   and a flow net constructed.
   Specific discharge determined
   from hydraulic gradient and field
   measurement of permeability.
   Wells were sampled monthly for
   nutrient content.
  -Measured daily or continuously
   depending on station, mainly
   with either 90° V notch weir
   and stilling well or bucket or
   culvert discharge and current
   meter.  Dollar Lake Stream
   Station was measured daily
   with either culvert discharge
   and bucket or 60  or 90  V
   notch weir and stilling basin
   or well.

  -Same sources and methods as for
   phosphorus loadings.  Nitrogen
   fixation was not included in
   the nitrogen loading estimates.

  -Estimated using four indirect
   methods as follows:

   1)  Using information from Vollen-
   weider (197Sa) assume phosphorus
   loading = three times measured lake
   concentration = three times mean
   outflow concentration;

-------
                      Table!? (continued).  SUMMARY OF METHODS USED TO CALCULATE
                                      NUTRIENT LOADINGS FOR UG OECD WATER BODIES
            Water Body
Nutrient Sources Considered by
US OECD Investigator in Nutrient
Loading Estimates
General Methodology Indicated by
Investigator to Determine the
Nutrient Loading to Water Body
            Waldo
            (continued)
o
01
                                         2)  Using watershed phosphorus
                                         export coefficients derived for
                                         undisturbed forest land in Upper
                                         Klamath Lake, Oregon  (Miller,
                                         unpublished data in Powers et al.,
                                         1975).
                                         3)  Using average precipitation
                                         data  for the lake and  snow
                                         analyses of Malueg ejt  al.  (197?)
                                         and assuming
                                         a)  all precipitation  into
                                             watershed eventually enters
                                             lake, or
                                         b)  only the precipitation equal to
                                             measured outflow plus estimated
                                             evaporation actually enters
                                             lake; and
                                         U)  Using total phosphorus soil
                                         export factors of Vollenweider and
                                         Dillon (1974), and assuming remainder
                                         of loading is direct precipitation
                                         onto  the lake surface.  The mean of
                                         the four estimated values was reported
                                         as the annual phosphorus loading.
                                        B)   Nitrogen  Loading:
                                       (NOTE:   Dry  fallout was not
                                               considered in nitro-
                                               gen  loading estimate;
                                               marsh  drainage and
                                               nitrogen  fixation con-
                                               sidered insignificant)
                                         Estimated using methods  2,  3a
                                         and  3b above.   (Method 1 not used
                                         because estimates of nitrogen
                                         retention in lake unknown.  Method
                                         4 not used because of lack  of  in-
                                         formation on soil loading of
                                         nitrogen to lake).

-------
          Table 12 (continued).   SUMMARY OF  METHODS USED TO CALCULATE
                           NUTRIENT LOADINGS FOR US OECD WATER BODIES
                        Nutrient  Sources  Considered by        General Methodology Indicated by
                        US  OECD Investigator in Nutrient      Investigator to Determine the
Water  Body              Loading Estimates                      Nutrient Loading to Water Body

Washington                  A)  Phosphorus Loading:

                           (NOTE:  Several sampling regimes  and  analytical
                                   methodologies were used by different
                                   investigators over the years, making a
                                   concise summary difficult)

                                   1957                          -All sewage plants and many tribu-
                                                                 taries to the lake sampled twice
                                                                 per week by the Seattle Engineer-
                                                                 ing Department.  Nutrient concen-
                                                                 trations, including total phos-
                                                                 phorus,  phosphate and particulate
                                                                 phosphorus were determined using
                                                                 methods  listed in APHA ejt al.
                                                                 (1971),  and earlier editions.

                                                                -METRO analyzed fewer tributaries
                                                                 (10) for fewer parameters (i.e.,
                                                                 total phosphorus, Kjeldahl nitro-
                                                                 gen and  nitrate plus nitrite nitro-
                                       i                          gen) approximately weekly.
                                                                -The two  major inlets and one minor
                                                                 inlet sampled biweekly by the US
                                                                 OECD investigator for total phos-
                                                                 phorus and phosphate (in 1957,
                                                                 these two major inlets supplied
                                                                 86% of total phosphorus loading.
                                                                 The total phosphorus loading is
                                                                 approximated by proportion).

-------
                   Table 12 (continued).   SUMMARY  OF METHODS USED TO CALCULATE
                                    NUTRIENT  LOADINGS  FOR  US  OECD WATER  BODIES
         Water Body
Nutrient Sources Considered by
US OECD Investigator in Nutrient
Loading Estimates
General Methodology Indicated by
Investigator to Determine the
Nutrient Loading to Water Body
         Washington
         (continued)
    Two sources of water flow data were used.  The major source
    was gage data published by USGS.  In 1957, the USGS was
    gaging the two major inlets + two smaller inlets.  The rest
    of the tributaries were determined by proportion with the
    watershed area.  A hydrological model was developed later for METRO
    and used until 1972 to estimate the Sammamish input.  Since
    1972, a regression equation that relates total Sammamish flow to
    stations that are gaged in the watershed has been used to determine
    the water flow.
                                     B)   Nitrogen Loading:
o
         Weir
     A)   Phosphorus Loading:

         1)  Rainfall
                                         -Same sources and methods as
                                         for phosphorus loading.  In  1957,
                                         the Seattle Engineering Depart-
                                         ment analyzed the  input water
                                         for "several nitrogen  components".
                                         In 1964, METRO analyzed the  samples
                                         for Kjeldahl nitrogen  and  nitrate plus
                                         nitrate nitrogen.   In  1970's the
                                         US OECD investigator has been ana-
                                         lyzing for nitrate, nitrite,
                                         ammonia and Kjeldahl nitrogen.
                                         The sources of flow data are the
                                         same as for the phosphorus loading.
                                                                         -Taken from Brezonik et al.  (1969)
                                                                          for rainfall at GainesvTTle,  60
                                                                          miles north of lake.

-------
                 Table 12 (continued).  SUMMARY OF METHODS USED TO CALCULATE
                                  NUTRIENT LOADINGS FOR US OECD WATER BODIES
        Water Body
Nutrient Sources Considered by
US OECD Investigator in Nutrient
Loading Estimates
General Methodology Indicated by
Investigator to Determine the
Nutrient Loading to Water Body
       Weir
       (continued)
        2)  Urban
                                        3)  Pasture

                                        4)  Forest
o
CO
                                        5)  Agriculture
  -Urban; runoff values taken from
   Weibel (1969) and represents
   averages for residential-light
   commercial areas found in study
   area.
  -Pasture and forest runoff values
   taken from Uttormark ejt aJL. (1974).
   In order to account for low nutrient
   binding capacity of sandy acid soils
   in study area, the "average" and
   "high" areal yield rates of Uttor-
   mark et al. (1974) were averaged
   for these two land-use classifica-
   tions .
  -Taken from estimates of Brezonik
   and Shannon (1971) based on the
   average fertilizer composition and
   application rate to citrus groves.
                                        6)   Septic Tank
                                        -Estimated using methods of Brezonik
                                         and Shannon (1971) .
                                         Average septic tank daily effluent
                                         flow of 475 1 , with total phos-
                                         phorus concentration of 8 mg/1, was
                                         assumed.  For lakeshore houses, it
                                         was assumed 10 percent of the phos-
                                         phorus was transported to the lake.
                                         For non-lakeshore houses, it was
                                         assumed one percent of the phos-
                                         phorus was transported to the lake.

-------
          Table 12 (continued).   SUMMARY  0! METHODS USED  TO CALCULATE
                           NUTRIENT  LOADINGS  FOR US  OECD WATER  BODIES
Water  Body
Nutrient Sources Considered by
US OECD Investigator in Nutrient
Loading Estimates
General Methodology Indicated by
Investigator to Determine the
Nutrient Loading to Water Body
Weir
(continued)
                                7)   Wetlands
                            B)   Nitrogen  Loading:
Wingra
    A)  Phosphorus Loading:

        1)  Precipitation
                                2)   Dry  Fallout
                                3)   Springflow
                                        -Net phosphorus contribution
                                         assumed zero.

                                        -Same sources and methods as above.
                                         For the septic tank nitrogen
                                         loadings, a total nitrogen concen-
                                         tration in the septic tank effluent
                                         of 35 mg/1 was assumed (Brezonik
                                         and Shannon, 1971).

                                         It was assumed 25 percent of the
                                         lakeshore homes nitrogen loading and
                                         10 percent of the non-lakeshore homes
                                         nitrogen loading were transported
                                         to the lake.
  -Rain and snow were collected in
   open bucket type containers which
   were put out when precipitation
   seemed imminent.
  -Estimated by exposing container to
   atmosphere for several days.
   During winter, bulk precipitation
   was measured rather than dry fall-
   out .
  -Monitored continuously by USGS
   where possible.  Samples collected
   every two weeks for phosphorus
   determinations.

-------
          Table 12 (continued).  SUMMARY OF METHODS USED TO CALCULATE
                           NUTRIENT LOADINGS FOR US OECD WATER BODIES
Water Body
Nutrient Sources Considered by
US OECD Investigator in Nutrient
Loading Estimates
General Methodology Indicated by
Investigator to Determine the
Nutrient Loading to Water Body
Wingra
(continued)
        4)  Urban Runoff




        5)  Groundwater


        6)  Marsh




    B)  Nitrogen Loading:
  -Determined by measurements taken
   from the Manitou Way Basin,
   especially during storm periods
   (Kluesener, 1972).

  -Considered insignificant
   (Kluesener, 1972).

  -Assumed marsh input loads roughly
   equal to marsh output loads.
   Therefore, marsh net phosphorus
   contribution is zero.

  -Same sources and methods as for
   phosphorus loadings.

-------
results are far from complete.  While all investigators reported
the nutrient sources they considered in their nutrient budget
estimates, in some instances sufficient detail was not given as
to exactly how the nutrient loadings were estimated.  For example,
if watershed land use nutrient export coefficients were used,
what was the distribution of land use types in the watershed?
How was the percentage of different watershed land use types cal-
culated?  How were the export coefficients calculated or estimat-
ed?  If nutrient inputs were measured directly, what analytical
methods were used?  What nutrients were measured?  What was the
sampling frequency?  How were the tributaries sampled?  How
many of the tributaries were sampled?  What percent of the tribu-
tary area was sampled?  These are major questions that must be
answered before the usefulness of US OECD eutrophication study
data, as applied in the Vollenweider phosphorus loading diagrams
and other loading diagrams, can be fully determined.

     The major nutrient input sources, according to most US
OECD investigators, were wastewater discharges, land runoff and
precipitation.  Most US OECD investigators also considered
groundwater inputs in their nutrient budget calculations, although
these inputs were generally considered insignificant nutrient
sources.  A summary of the various nutrient sources considered
in the nutrient loading calculations, as indicated by the US OECD
investigators, is presented in Table 13.

METHODS FOR EVALUATION OF ESTIMATES OF US OECD WATER BODY NUTRIENT
LOADINGS

     Suffiently detailed information concerning the methodology
used in estimating the nutrient budgets for the US OECD eutro-
phication study water bodies was not available in most cases.
As a result, several independent methods were employed by these
reviewers in an attempt to check the reasonableness of the nutri-
ent loadings reported by the US OECD investigators.  These methods
include the use of several relationships developed by Vollen-
weider (which relate phosphorus loadings to mean water body phos-
phorus concentrations) and the use of watershed nutrient export
coefficients and land usage patterns within the watershed of a
water body to predict phosphorus and nitrogen loadings.  These
methods were not developed as an absolute guide for evaluating
the accuracy of the US OECD investigators'  nutrient loadings, but
rather are meant to serve as a basis for checking on the reason-
ableness of these loadings, with the goal of detecting any pos-
sible major errors or unusual water body situations.  An identifi-
cation key for the US OECD water bodies is presented in Table 14.
This key will be used in all subsequent figures to identify the
US OECD water bodies.

                               Ill

-------
                                Table  13   SUMMARY  OF NUTRIENT  SOURCES  CONSIDERED
                                IN US  OECD WATER BODY NUTRIENT LOADING ESTIMATES
M

Water Body
Blackhawk
Brownie
Calhoun
Came lot -Sherwood
Complexb
Canadarago
Cayuga
Cedar
Cox Hollowb
Dogfish
Dutch Hollowb
George
Harriet
Isles
Kerr Reservoir
Lamb
Urban Precipi-
and/or Ration Dry Fallout
T , . Waste Rural onto pnto
P Water Land Water Body Water Body
State Discharges Runoff Surface Surface
E + + + +
E + + +
E + + +
E + + + +
E + + + +
M + + + +
E + + +
E + + + +
0 + + + +
E + + + +
0-M + + + +
E + + +
E + + +
E-M + + + 0
0 + + + +
Ground
Water Woodland
Seepage Runoff
ft +
+ +
+ +
+
0 0
ft +
+ +
+ +
0 +
0 +
+ +
+ +
0 +
0 +
Marsh Nitrogen
Drainage Fixation
+
+ ft ft
+ ft ft
+
0
-
+ ft ft
+
0 **
-
+ ft ft
+ ft ft
+ 0
0 ft ft

-------
     Table 13 (Continued).  SUMMARY OF NUTRIENT SOURCES
CONSIDERED IN US OECD WATER BODY NUTRIENT LOADING ESTIMATES

Water Body
Meander
Mendota
Michigan
Minnetonka
Potomac
Estuary
Redstone
Sallie
Sammamish
Shagawa
Stewart5
Tahoe
Twin Lakes
Twin Valley5
Virginia
Trophic
State3
0
E
0-M
E--M

U-E
E
E
M
E
E
U-0
E
E
E
Waste
Water
Discharges
4
4
4
+

+
+
+
4
4
+
4
4
4
4
Urban Precipi-
and/or tation Dry Fallout
Rural onto onto
Land Water Body Water Body
Runoff Surface Surface
44 +
4 + +
44 +
44

40
44 4
44- -
44 _
+ 4- 4
44 4
44
44 A
44 4
44 4-
Ground
Water
Seepage
0
4
4
-

0
4-
4
0
-
4
0
4
4
4
Woodland
Runoff
4
0
4
-

4
4
4-
4-
4-
4
4
4
4-
4-
Marsh
Drainage
0
0
4
4

-
4
4
4
0
4
4
0
+
4-
Nitrogen
Fixation
z *
4
-
* f:

-
-
-
-
_
-
-
*
_
_

-------
                                  Table 13(Continued).   SUMMARY  OF  NUTRIENT  SOURCES

                              CONSIDERED  IN US OECD WATER  BODY NUTRIENT  LOADING ESTIMATES



Water Body
Waldo
Washington
(1974)
Weir
Wingra
Urban Preoipi-
and/or tation Dry Fallout
^ , . Waste Rural onto onto
.ropnic Water Land Water Body Water Body
State Discharges Runoff Surface Surface
U-0 + + +
M + +

M + + +
E + + + +


Ground
Water Woodland Marsh Nitrogen
Seepage Runoff Drainage Fixation
+ + o -
+ + + -

+ + 0
+ + +
 EXPLANATION:

 + = considered in nutrient budget calculations
 - = not considered in nutrient budget calculations
 0 = considered to be insignificant in nutrient budget
 * = considered in nutrient budget calculation, but significance unknown
* * = nitrogen budget not calculated
  Investigator indicated trophic state:
    E - eutrophic
    M = mesotrophic
    0 = oligotrophic
    U = ultra

  Nutrient budget calculated from watershed land use nutrient export coefficients.

-------
Table 14.
IDENTIFICATION  KEY  FOR
US OECD WATER BODIES

Water Body Identification Investigator-
Number Indicated
Trophic Status
Blackhawk
Brownie
Calhoun
Came lot -Sherwood
Canadarago
-1968
-1369
Cayuga
-1972
-1973
Cedar
Cox Hollow
Dogfish
-1971
-1972
Dutch Hollow
George

Harriet
Isles
Kerr Reservoir
Whole Reservoir

-Roanoke Arm
-Nutbush Arm
Lamb
-1971
-1972
1
2
3
4

5 -A
5-B

6 -A
6-B
7
8

9
10
11
12

13
14

15

16
17

18
19
Eutrophic
Eutrophic
Eutrophic
Eutrophic

Eutrophic


Mesotrophic

Eutrophic
Eutrophic

Oligotrophic
Oligotrophic
Eutrophic
Oligotro ohic-
Mesotropnic
Eutrophic
Eutrophic
Eutrophic-
Mesotrophic




Oligotrophic
Oligotrophic
Location
Wisconsin
Minnesota
Minnesota
Wisconsin
New York


New York


Minnesota
Wisconsin

Minnesota

Wisconsin
New York

Minnesota
Minnesota
North
Carolina,
Virginia


Minnesota


                115

-------
Table 14
          (Continued)  IDENTIFICATION  KEY  FOR
           US OECD WATER BODIES
Water Body Identification Investigator-
Number Indicated
Trophic Status
Meander
-1971
-1972
Mendota
Michigan (open waters)
-1971 , T = 30 yrs
-1974 ' U
-1971
-1974 } TU = 10° yrs

20
21
22
2 3 -A
24-A
23-B
24-B

Oligotrophic
Oligotrophic
Eutrophic
(changing)
Oligotrophic
Oligotrophic


Location
Minnesota


Wisconsin
Michigan,
Wisconsin


Michigan (nearshore waters)
-1971
-1974
Lower Lake Minnetonka
-1969
-1973
Potomac Estuary
Whole Estuary
-Up'per Reach
-Middle Reach
-Lower Reach
Redstone
Sallie
Sammamish
Shagawa
Stewart
Tahoe

23-C
24-C

25
26
27
28
29
30
31
32
33
34
35
36




Eutrophic
Eutrophic
(changing)
Ultra -Eutrophic



Eutrophic
Eutrophic
Mesotrophic
Eutrophic
Eutrophic
Ultra-
Oligotrophic


Minnesota


Maryland ,
Virginia



Wisconsin
Minnesota
Washington
Minnesota
Wisconsin
California,
Nevada
                  116

-------
Table 14 (Continued)   IDENTIFICATION KEY
         FOR US OECD  WATER BODIES

Water Body Identification Investigator-
Number Indicated
Trophic Status
Twin Lakes
East Twin Lake
-1972
-1973
-1974
West Twin Lake
-1972
-1973
-1974
Twin Valley
Virginia
Waldo
Washington
-1957
-1964
-1971
-1974
Weir
Wingra
37
38
39
40
41
42
43
44
45
46
47
48

49
50
51
52
53
54
Eutrophic
(changing)

Eutrophic
Eutrophic
Eutrophic

Eutrophic
Eutrophic
Eutrophic
Eutrophic
Eutrophic
Ultra-
Oligotrophic

Eutrophic
Eutrophic
Mesotrophic
Mesotrophic
Mesotrophic
Eutrophic
Location
Ohio








Wisconsin
Wisconsin
Oregon
Washington




Florida
Wisconsin
                     117

-------
Vollenweider Mean Phosphorus/Influent Phosphorus And  Hydraulic
Residence Time Relationship

     The first method used by these reviewers to check the reason-
ableness of the US OECD eutrophication study phosphorus loading
estimates involved the use of the relationship between the average
influent phosphorus concentration and the mean phosphorus concen-
tration in the water body.  Equation 20 may be rearranged as
follows:
                CP]oo/(L(P)/qs) = 1/(1 + /zTq^)         (25)

Recalling L(P)/q  = [P] and z/q  = T , then Equation 25 becomes
                S              S    CO

                [p]/rp] = i/d +~T)                 (26)
                   ra
     According to Vollenweider (1975b; 1976a), the average influ-
ent phosphorus concentrations are generally higher than the mean
water body phosphorus concentrations because of the continuous
loss of phosphorus to the sediments.  In a highly flushed water
body (i.e., hydraulic residence time, T , < 0.5 yr) , which would
exhibit very little relative sedimentation of phosphorus because
of the rapid flow of phosphorus through the water body, the ratio
of the mean phosphorus concentration to the influent phosphorus
concentration approaches unity.  With less rapidly flushed water
bodies, there is an increasing involvement of the input phos-
phorus with the water body metabolism and a resultant deviation
of this ratio from unity.  This deviation can become positive or
negative, depending on whether phosphorus accumulates in the
water phase or the sediment phase of the water body.  In actual-
ity, the ratio of the water body mean phosphorus concentration
to the average influent phosphorus concentration defines the
ratio of the re'sidence time of phosphorus to the residence time
of water (i.e., ^p/Tto::'1Tr ) •> though in principle this definition
applies to any substance flowing into a water body.  It can also
be used to check on the phosphorus sedimentation rate  (Vollen-
weider,  1976a). The derivation and implications of the relative
phosphorus residence time, TT^ , have  been discussed in  an earlier
section of this report (See Equations 13-16).

     The reasonableness of the US OECD eutrophication  study phos-
phorus loading estimates can be checked with the use of Equation
26.  A water body's influent phosphorus concentration, [p]  ,  can
be calculated as L(p) /qs . The ratio of its mean phosphorus to  in-
fluent phosphorus  concentration, [P]/[pJ, can then be compared
to its hydraulic residence time expression,  1/(1 + /T^) •  The
relationship expressed above  in Equation 26  can be used as a
check on the phosphorus loading estimates since the influent
phosphorus concentration is a  function of the phosphorus loading.
Any major  deviations of [P]/[P] from 1/(1 +>|rT^p would  make the
reported phosphorus loading data suspect.  Vollenweider has used
this relationship successfully to trace loading errors in the
phosphorus budgets for Lakes  Constance (Vollenweider,  1975c)  and
Lunzer See (Vollenweider,  1975d).  The use of Equation 26 to  check

                                 118

-------
on the accuracy of a water body's phosphorus loading estimate
requires that the water body mean phosphorus concentration be
accurately known.  No equivalent relationship has been derived
by Vollenweider for checking nitrogen loading estimates, al-
though a similar approach would likely be applicable.

     The relationship expressed in Equation 26 has been applied
to the US OECD eutrophication study phosphorus loading estimates.
The pertinent data are presented in Table 15.  A missing water
body identification number indicates that necessary data for
the relationship expressed in Equation 26 were not available for
a given water body for a particular time period.  For example,
there is insufficient data for Dogfish Lake-1971 (Identification
Number 9).  Consequently, it was not included in Table 15.
Similar reasoning holds for any missing water body identification
numbers in any of the tables in this report.  Refer to Table 14
for identification of any water bodies and/or time periods not
included in a given table or figure in this report.  A plot
(Figure 14-) has been prepared which graphically illustrates the
relationship indicated in Equation 26.  The US OECD data, as
reported by the US OECD investigators, are also presented in
Figure 14-.  If a data range was reported for a water body, the
mean value was used in all calculations.  The solid line in
Figure 14 signifies a perfect agreement between [P]/[P] and
!/(!+ /"r^") .   According to the Vollenweider relationship
(Equation 26), if the phosphorus loading was overestimated (i.e.,
the phosphorus loading L(P) is actually smaller than that re-
ported by the US OECD investigator), then the water body would
plot below the solid line.  Conversely, if the phosphorus load-
ing were underestimated (i.e., the phosphorus loadings are actu-
ally higher than those reported by the investigator), the water
body would plot above the solid line.  The broken lines indicate
the degree of possible over- or underestimation of the US OECD
investigator-indicated phosphorus loadings relative to that
predicted by the hydraulic residence time expression in Equation
26.  The "+2x" broken line below the solid line indicates the
US OECD investigator-indicated phosphorus loading estimate may
have been overestimated (i.e., +) a factor of 2 (i.e., 2x) .  Con-
versely, the "-3x" broken line above the solid line indicates
the phosphorus loading estimates may have been underestimated (-)
by a factor of 3 (3x).  The shaded zone between ± 2x indicates the
range within which the phosphorus loadings were considered to be
reasonable by these reviewers.  The basis for the choice of this
range of acceptable deviation will be discussed further in a follow-
ing section.

     As can be seen in Figure 14, almost no water bodies fall
directly on the solid line.   However, many of the water bodies
fall within the shaded area between the broken lines representing
a + two-fold possible phosphorus loading estimate error.  This indi-
cates the US OECD phosphorus loading estimates generally appear to

                                 119

-------
Table 15.  US OECD DATA FOR VOLLENWEIDER'S MEAN PHOSPHORUS/
           INFLUENT PHOSPHORUS AND HYDRAULIC RESIDENCE TIME
           RELATIONSHIP
Trophic
Water Body State3
Blackhawk (l)e
Brownie (2)
CaLhoun (3)
r-o Camelot-Sherwood(4
o
Canadarago ( 5 )
Cayuga (6)
Cedar (7)
Cox Hollow (8)
Dogfir.h (10)
Dutch hollow (11)
George ( ] 2 )
Harriet (13)
Isles (14)
Kerr Reservoir
ivoanoke Arm (16)
flutbush Arm (17)
E
E
E
) E

E
M
E
E
0
E
0-M
E
E
E-M
-
_
Phosphorus
Loading
(mg P/m /yr)
2220
1180
860
2350-2680

800
800
350
1620-2080
20
950-1010
70
710
2030

5200
700
Hydraulic
Loading, q
b (m/yr)c
9
3
2
21.4

12
6
1
5.4
1
1
2
3
4

51
1
.8
.4
.94
-33.3

.8
.3
.8
-7 .6
.14
.67
. 25
.67
.5

.5
.6
Influent
Phosphorus
Concentration
[P]
(mg/m3)d
227
347
292
70 .6-125

62 .4
127
189
213-385
17.5
569-605
31.1
194
451

101
435
Mean
Phosphorus
Concentration ,
[P] CP]
(mg/m3)b
50-120
_
106f
30-40

40-50
20
55f
60-100
10
120-400
8 .5
62f
110f

30
30
[P]
0.22-0.52
_
0 .36
0 .24-0.57

0.64-0 .80
0 .15
0 .29
0 .16-0.47
0 .57
0 .20-0 .70
0.27
0. 32
0 .24

0 .30
0 .07
1
" + V\?
0 .58
0.41
0 .34
0 .73-0 .77

0 .56
0 .25
0 .36
0.54-0.58
0 .48
0 .43
0.26
0.39
0 .56

0 .69
0 .31

-------
IS (continued).  US OFCD DATA FOR VOLLKNWF: JDER ' 5 MEAN
   PHOSPHORUS/INFLUENT piio.srnoRus AND  HYDRAULIC
   RESIDENCE TIMI: RELATIONSHIP

Trophic
Water Body Statea
Lamb (19)
Meander (21)
Mendota (72)
Michigan
Open Waters(23-A)
(23-B)
0
0
E
0
Phosphorus Hydraulic
Loading Loading, q
(mg P/m2/yr)b (m/yr)c
30
30
1200
140
140
1 .74
1 .85
2.67
2 .8
0 .84
Influent
Phosphorus
Concentration
^\
(mg/ni )d
17 .2
16 .2
450
50
367
Mean
Phosphorus
Concentratior
[P]
(mg/m3)b
12-13
9-12
ISO
13
13
i ,
[P]
m
0.69-0.76
0 .56-0 .74
0.33
0.26
0.08
1

-------
Table 15 (continued).  US OECD DATA  FOR  VOLLENWEIDCR'S MEAN
         PHOSPHORUS/INFLUENT PHOSPHORUS  AND  HYDRAULIC
         RESIDENCE TIME RELATIONSHIP
Trophic
Water Body State3
Tahoe (36)
East Twin
1972 (39)
1973 (40)
1974 (41)
West Twin
1972 (43)
1973 (44)
1974 (45)
Twin Valley (46)
Virginia (47)
Waldo (48)
Washington
1957 (49)
1964 (50)
1971 (51)
U-0
E
E
E
E
E
E
E
E
U-0
E
E
M
Phosphorus
Loading
(mg P/m2/yr)b
50
700(672)^
500(472)
700(816)
400(419)
300(181)
300(316)
1740-2050
1150-1480
17
1200
2300
430
Influent
Phosphorus
Hydraulic Concentration
Loading, qg fjTj
(m/yr) (mg/m )
0 .45
6.25(7.40)3
5 .56(7.19)
10.0(9.31)
2.71(0.79)
2.41(0.64)
4.34(1.03)
7.6-9.5
0.61-1.89
1.71
13.8
13.8
13.8
112
112 (91) 3
89.9(66)
70(76)
148(123)
124(65)
69.1(75)
183-270
608-2426
9.9
87 .0
167
31.3
Mean
Phosphorus
Concentration
[P]
(mg/m3)b
3
80 (83)1
80 (78)
80(77)
120(122)
110(107)
100(97)
60-70
20-150
<5h
24
66
18
[P]
[Pi
0 .03
0.71 (0.91^
0.89(1.81)
1.14(1.01)
0 .81(0.99)
0.89(1.65)
1.45(1.29)
0.22-0.38 0
0.01-0.25 0
< 0 .5
0.28
0.40
0.58
1
;i +/rj)
0 .04
0 .53 (0.54)
0.51(0.54)
0.59 (0.58)
0 .44 (0.47)
0.43(0.45)
0 .50
.58-0 .61
.37-0.51
0 .18
0 .39
0.39
0 .39

-------
                     Table 15 (continued).  US OECD DATA FOR VOLLENWEIDER'S MEAN
                              PHOSPHORUS/INFLUENT PHOSPHORUS AND HYDRAULIC
                              RESIDENCE TIME RELATIONSHIP



Water Body
Weir (53)
Wingra (54)


Trophic
State3
M
E

Phosphorus
Loading
(mg P/m2/yr)b
140
900

Hydraulic
Loading, q
(m/yr)c
1.5
6.0
Influent
Phosphorus
Mean
Phosphorus


Concentration Concentration,
[P]

-------
o
tr
i-
z
LJ
O
Z
O
O
co
:D
cr
o

o.
CO
O
X
Q.

V-
z
              -20X
                      -iox
cr
h-
z
LL)
O
Z
O
o

CO
z>
cr
o

a.
CO
O
X
a.
LJ
2
2.4




2.2




2.0
O.8
0.6
0.4
0.2
                                 -6X
    INVESTIGATOR-INDICATED

        TROPHIC  STATg:

        - EUTROPHIC/

      /A - MESOTROPHIC

      O - OLIGOTROPHIC

      I - LESS THAN7
                                                           -3X
UNDERESTIMATION  -
il////V*-bS^ I»^—'
W/-Q?^.^^~\7
                                                            -2X
                      + 2X




                      -I-ZX


                       4X


                      + &X.

                      + \ox

                      42OX
 Figure  14.   Evaluation of Estimates of US OECD  Water Body
              Nutrient Loadings:   Vo 11 enwei de'r  Mean  Phosphorus/
              Influent Phosphorus  and Hydraulic  Residence
              Time Relationship
                             124

-------
be of a reasonable nature, based on the Vollenweider relation-
ship (Equation 26).  Considering the multitude of methods used
in estimation of the phosphorus loadings (Table 12),this initial
agreement between the phosphorus loadings as indicated by the
US OECD investigators and the phosphorus loadings as indicated
by the Vollenweider relationship (Equation 26) is reassuring and
provides some affirmation of the Vollenweider loading diagram ap-
proach to establishing the critical phosphorus loading levels
and relative trophic conditions of water bodies.  Equation 26
will be discussed in greater detail in relation to the Vollen-
weider phosphorus loading diagrams presented in subsequent sec-
tions of this report.

Watershed Land^ Use Nutrient Export Coefficients

     The other principal method used by these reviewers for
checking the reasonableness of the phosphorus loading estimates,
as well as the nitrogen loading estimates, reported by the US
OECD investigators was to compare the reported loadings with
those computed using watershed nutrient export coefficients.
The nutrient export coefficients used to estimate the nutrient
loadings from a given watershed would depend on the land usage
pattern within the watershed.  Because no relationship equivalent
to Equation 26 has been derived for nitrogen loadings , the use
of watershed nitrogen export coefficients represents the only
independent method available to these reviewers for checking the
accuracy of the nitrogen loadings reported by the US OECD inves-
tigators .

     This  procedure involves utilization of the information
available  on land usage within a lake's or impoundment's water-
shed and the nutrient coefficients which are applicable to the
various land uses within that watershed.  For example , a hectare
of corn or a suburban subdivision are known to yield a relatively
constant amount of aquatic plant nutrients over the annual cycle
(see Sonzogni and Lee (1974), for further discussion of this ap-
proach) .  The use of this approach for computing nutrient load-
ings to a  water body requires an accurate estimation of the water
body's watershed area and the land usage pattern within the
watershed.  The US OECD investigators reported watershed land
usage in varying degrees, with some investigators producing only
sparse watershed land usage data, while others went into great
detail concerning land usage within the watershed.

     Uttormark et al_. (1974), based on the results of their exten-
sive survey, have reported there is little justification for
the delineation of land usage within direct drainage basins be-
yond four  categories:  urban, forest, agriculture and wetlands.
Available  data are too fragmentary and variable to warrant fur-
ther subdivision of land usage categories, according to Uttor-
mark et_ al.  (1974).  The US EPA has taken the same general
approach in categorizing watershed land usage types as urban,

                                 125

-------
agriculture, mostly agriculture, forest,  mostly forest and mixed
(US EPA, 1974c;  1975c).   Vollenweider (1977)  has recently indi-
cated, based on studies of German watershed land usage, that a
distinction between arable land and pastures  and meadows may be
useful because these two classes of land  use  types export dis-
tinctly different quantities of phosphorus and nitrogen from the
watershed.  However, it is noted that the values reported by
Vollenweider are considerably above the North American values
reported by Uttormark et_ al. (1974).  Typical values of water-
shed nutrient export coe"f fTcients are presented in Table 16.
It is noted that while wetlands can act as sinks or sources of
nutrients, depending on the season of the year, in general the
net nutrient contribution from wetlands is considered to be zero
(Sonzogni and Lee, 1974; Uttormark et_ al. , 1974, Lee e_t al_. , 1975).

     Table 16 indicates that several different nutrient export
coefficients, varying widely in several cases, were available
for each watershed land use category (i.e., 0.1 g/m^/yr (Sonzogni
and Lee, 1974) vs. 0.03 g/m2/yr (US EPA,  1974c) for urban phos-
phorus export coefficient).  As a result, the coefficients chosen
to check the reported US OECD nutrient loadings are based largely
on the experience of these reviewers and  also on the regional
nature of several of these values.  For example, it was felt by
these reviewers that the urban phosphorus and nitrogen export co-
efficients of Sonzogni and Lee (1974) represent a reasonable
average of the values reported by Uttormark et al. (1974) and by
the US EPA (1974c).  The US EPA urban phosphorus~and nitrogen ex-
port coefficients were based on studies done  in 473 subdrainage
areas in the eastern US.  The coefficient of  Sonzogni and Lee
(1974) is also regional in that it was derived for the Lake Men-
dota, Wisconsin, watershed.  However, it  is more in agreement with
that reported by Uttormark et al. (1974)  than is the US EPA (1974c)
value.  While the, coefficients of Uttormark et al. are also
derived from studies confined mainly to the nortTTeastern and upper
midwestern US, they are also based on several studies done in the
southern and western US and, therefore, represent more of a
'national average' than do the values of  Sonzogni and Lee or the
US EPA.  Consequently, a certain bias was given to the values of
Uttormark et al. (1974) as a reference national average value,
even' though~the~y were based on studies confined largely to the
upper midwestern and northeastern US.
                                                     2
     A rural/agriculture phosphorus value of 0.05 g/m /yr was
taken as an average of the values of Sonzogni and Lee (1974) and
both Uttormark ejt al. (1974) and the US EPA (1974c).  A rural/
agriculture nitrogen export coefficient of 0.05 g/m2/yr was used
because of the agreement between the value of Sonzogni and Lee
and that of Uttormark et al,  The forest  phosphorus export
coefficient of Uttormark" et" al. was thought to be too high, based
on the experience of these reviewers and  on the "mostly forest"
value reported by the US EPA.  Consequently,  the US EPA (1974c)
forest phosphorus export coefficient of 0.01  g/m2/yr was used by
these reviewers.  A forest nitrogen export coefficient of

                                 126

-------
      Table 16   TYPICAL VALUES OF WATERSHED NUTRIENT EXPORT
                             COEFFICIENTS
Watershed
Land Usage
Source :
and Lee
Sonzogni
(1974)
Source :
et_ al_.
: Uttormark
(1974)a
Source : US
Cl974c)
EPA
                 A. Total Phosphorus (g P/m /yr)


Urban                   0.1                0.15            0.03       ,
Rural/Agriculture       0.07               0.03            0.03  (0.02)D
Forest                   -                 0.02            0.01  (0.02)
Wetlands         Net nutrient contribution is considered tc be zero.

Other:
  Rainfall onto
  water body surface    0.02

  Dry fallout onto
  water body surface    0.08                -                         ,
                                                          .mixed  = 0.02


                 B.  Total Nitrogen (g N/m /yr)


Urban                   0.5                0.5             0.8
Rural/Agriculture       0.5                0.5             1.0(0.6)D
Forest                   -                 0.25            0.4(0.4)°
Wetlands         Net nutrient contribution is considered to be zero.

Other:
  Rainfall onto
  water body surface    0.8

  Dry fallout onto
  water body surface    1.6                 -                  -
                                                          mixed - 0.6


a"Average" value indicated by Uttormark e_t al.  (1974).
 Mostly agriculture; other types present.
Q
 Mostly forest ; other types present.
 Does not fit into any of the other watershed land use categories.
                             127

-------
       2
0.3 g/m /yr was taken as an average of the values reported by
Uttormark et al. and the US EPA.  The one exception to these
values is that the "low" nitrogen export coefficients reported
by Uttormark et al.  (1974) were used as a check on the reported
nitrogen loadTngsr~of the US OECD water bodies located in the
western US.  These low values were used because most water bodies
in the western US tend to be nitrogen-limited  with respect to
aquatic plant nutrient requirements.  It was felt by these re-
viewers that the low nitrogen values were more accurate than the
"average" values reported by Uttormark et al. (1974).  These low
nitrogen values were used for calculating' tTTe nitrogen loadings
for Lakes Tahoe, Waldo, Sammamish and Washington.

     The values for the nutrient contributions to the US OECD
water bodies from precipitation and dry fallout directly onto
the water body surface, if not indicated by the investigator,
were taken from Sonzogni and Lee (1974).  While precipitation
and dry fallout nutrient contributions likely vary from location
to location, the portion of nutrients contributed by precipitation
or dry fallout onto a water body's surface was usually small,
compared to the magnitude of the other input sources.  Conse-
quently, it was not considered a serious source of error to use
the values reported by Sonzogni and Lee (1974) .

     A summary of the watershed land use nutrient export coef-
ficients used by these reviewers as a check on the reported US
OECD water body nutrient loadings is presented in Table 17.

Table 17.  WATERSHED NUTRIENT EXPORT COEFFICIENTS USED TO CHECK
           US OECD NUTRIENT LOADINGS
Watershed               Watershed Export  Coefficient
Land Use                         (g/m^/yr)

                        A. Total Phosphorus
Urban                            0.1
Rural/Agriculture                0.05
Forest                           0.01
Other:
  Rainfall                       0.02
  Dry Falout                     0.08

                        B. Total Nitrogen

Urban                            0.5  (0.25)a
Rural/Agriculture                0.5  (0.2)a
Forest                           0.3  (0.1)a
Other:
  Rainfall                       0.8
  Dry Fallout                    1.6

aExport coefficients used  in  calculating  nitrogen  loadings  for
 US OECD water bodies  in western US  (i.e., Lakes Tahoe, Waldo,
 Sammamish and Washington).

                                128

-------
     In order to use these watershed land use and atmospheric
nutrient export coefficients, the percentage of each of the four
land use types in the watershed was determined from the data
provided by the US OECD investigators.  In some cases, an inter-
pretation of a given watershed land usage type was used for this
report if the US OECD investigator's description did not fit
into any of the four watershed land use categories reported by
Uttormark et al.  (1974) (i.e., "residential," "commercial,"
"industrial,"""public, semipublic transportation" and "mining"
all being placed in the 'urban' category; "outdoor recreation"
put into the 'forest' category, etc.).  In general, the effect
of the occasional liberal usage of watershed land use categories
by these reviewers have tended to overestimate the nutrient load-
ings to the US OECD water bodies to some extent.  In most cases,
the investigator's reported watershed land usages conformed to
the general categories defined by Uttormark et al. (1974).  How-
ever, the methods employed in determining the watershed land usage
patterns, or the sources of the watershed land usage data, if it
was not directly determined, were usually not indicated by the
US OECD investigators.  Any other nutrient contribution values used
in this portion of the report were those supplied by the US OECD
investigators for their particular water bodies.  These included
wastewater discharges, groundwater inputs, spring inputs, nitrogen
fixation (for nitrogen loading estimates) and marsh drainage.

     The total phosphorus and total nitrogen loadings , as calcu-
lated using watershed land use nutrient runoff coefficients, are
presented in Table 18.  The US OECD investigator-indicated total
phosphorus and total nitrogen loadings are included in Table 18
for comparison with the loadings derived from watershed land use
nutrient export coefficients.  The ratio of the export coefficient-
derived nutrient loadings to the investigator-indicated loadings
is also presented in Table 18.  A ratio of one indicates agreement
between the investigator-indicated nutrient loadings and the nu-
trient loadings calculated from watershed nutrient export coef-
ficients .  A ratio greater than one indicates the investigator-
indicated nutrient loadings may have been underestimated, rela-
tive to the nutrient loading estimates obtained from the water-
shed land usage calculations.  That is, the investigator-indicated
nutrient loading is lower than the loading based on the watershed
nutrient export coefficients listed in Table 17.  Conversely, for a
ratio less than one, the possibility of a nutrient loading over-
estimation is indicated.

     According to Piwoni and Lee (1975) the nutrient loadings
for Lakes Blackhawk, Camelot-Sherwood, Cox Hollow, Dutch Hollow,
Redstone, Stewart, Twin Valley and Virginia were calculated using
nutrient export coefficients derived by Sonzogni and Lee (1974).
Since the nutrient export coefficients derived by Sonzogni and
Lee (1974) are different for some land use types than those used
by these reviewers, comparing the reported nutrient loadings for
                                129

-------
                           Table 18.   US OECD NUTRIENT LOADINGS  CALCULATED USING
                                      WATERSHED NUTRIENT EXPORT  COEFFICIENTS
Ratio of Export
Loadings Coefficient
b Calculated Investigator- Loadings to
Point-Source Non-Point Source Loading via Export Indicated Investigator-
Loading3 (g/yr) Coefficients Loadings Indicated
Water Body (g/yr) Urban Rural Forest Other (g/»2yr) (g/™ /yr)a Loadings
A. PHOSPHORUS LOADINGS:
Brownie Watershed land usage data not available
(2)d


h- '
co
CD
Calhoun Watershed
(3)
Canadarago 2.8x10
Cayuga 6.39x10
(6)
land usage data not' available
6.02xl05
7.32xl06
(Includes
commercial
4.3xl06 6.02xl05 7.6xl05 1.2 0.8
5.95xl07 0 1.7xl07 0.9 0.8
(Includes
, active £
1.5
1.1
Cedar
 (7)
Dogfish
 (10)

George
 (12)
Harriet
 (13)
            industrial  inactive
            mining,pub- agriculture)
            lie and
            transportation)
Watershed land usage  data  not  available


  0            00       5.9xlOc

Watershed land usage  data  not  available

Watershed land usage  data  not  available
2.9x10
         0.1
                       0.02

-------
Table 18 (continued).   US OECD NUTRIENT LOADINGS CALCULATED
         USING WATERSHED NUTRIENT EXPORT COEFFICIENTS

Water Body
Isles
(14)
Kerr Reser-
voir (Whole
reservoir )
(15)
Lamb
(19)
Meander
(21)
Mendota
(22)
Point-Source
Loading3
(g/yr)
Non-Point
Urban Rural
Watershed land usage data not
7
2. 34x10



0

0
0

R ft
2x10 3x10



0 0

0 0
7.81xl06 2.7x10

Source Loading
(g/yr)
Forest
available
a
1. 2x10



1.6xlOU

1. 34xl04
7 6.51xl04

b
Other0

7
3.2x10



4xl04

3.6X104
1.09xl07
( Includes
Loadings
Calculated
via Export
Coefficients
(g/nryr)


4.0



0.1U

0. 14
1.2

Investigator-
Indicated
Loadings
(g/m2/yr)a


4.0



0.03

0.03
1.2

Ratio of Export
Coefficient
Loadings to
Investigator-
Indicated
Loadings


1. 0



4.7

4.7
1.0












grouridwater,



Michigan
Lower Lake
Minnelonka
Potom.ic
EL. tuary



Watershed lar




id usage data not

Watershed land usage data not
H x 109
(Median flow



available

available
1.86xl08 7.46xl08 2.05xl08
regime)

baseflow,
storm
drainage )



9.67xl07

E





5.4







5. 0







1.1









(entire <;b tuary)
(27)









-------
Table 18 (continued).   US  OECD NUTRIENT LOADINGS CALCULATED
         USING WATERSHED NUTRIENT EXPORT COEFFICIENTS

Water Body
Sallie
(32)
Sanunamish
( following
diversion of
sewage )
(33)
Shagawa
(34)
Tahoe
(36)
Twin Lakes
(East Twin £
West Twin
combined)
1972
(39 £ 43)
1973
(40 £ 44)
1974
(41 £ 45)
Ratio of Export
Loadings Coefficient
b Calculated Investigator- Loadings to
Point-Source Non-Point Source Loading via Export Indicated Investigator-
Loading3 (g/yr) Coefficients Loadings Indicated
(g/yr) Urban Rural Forest Other0 (g/m yr) (g/m2/yr)a Loadings
2!oixlo7 4.5xl06 3.38xlO? 3.45xl06 1.15xl06 9.4-12.1 1.5-4.2 2.2-8.1
5xl05 2.75xl06 3.75xl05 2.15xl06 2.6xl06 0.4 0.7 0.6



5.18xl06 1.7xl06 1.31xl05 2xl06 8.86xl05 1.1 0.7 1.6
7 67
0 2.88x10 0 4.72x10 5x10 0.17 0.05 3.4




r o r _
0 1.0x10 0 8.02x10 2.4x10 0.57 0.5l(0.53)K i.id.lp
r Q C
0 1.0x10 0 8.02x10 2.4x10 0.57 0.40(0.31) 1.4(1.8)
r . T r
0 1.0x10 0 8.02x10 2.4x10 0.57 0.45(0.54) 1.3(1.1)


-------
Table 18 (continued).  US OECD NUTRIENT  LOADINGS  CALCULATED
         USING WATERSHED NUTRIENT EXPORT COEFFICIENTS



Water Body
Waldo
(48)
Washington
(assumed 90
forest and
10 percent

1957
(49)
1964
(50)
1971
(51)
1974
(52)
Weir
(53)

Wingra
(54)


Point- Source
Loading3
(g/yr)
0


percent

urban )
7
5. 7x10

1. 04xl08

0

0

0


0



Non-Point
Urban Rural
0 0





7
1.61x10 0

l.GlxlO7 0

1.61xl07 0

i.eixio7 o

3.68xl05 1.7xl06


l.OSxlO6 0




K
Source Loading"
(g/yr)
Forest
5. 2xl05





7
1.45x10

1.45xlO?

1. 45xl07

1.45xlO?

8.74xlOM


3.13xl04

Other0
2.88xl06





c
8.8x10

8.8xl06

8.8xl06

8.8xl06

3.5xl06
( includes
Loadings
Calculated
via Export
Coefficients
(g/m yr)
0.12






1.09

1.63

0.45

0.45

0. 24


Ratio of Expon
Coefficient
Investigator- Loadings to
Indicated
Loadings
(g/m2/yr)a
0.02






1. 2

2.3

0.43

0.47

0.14

Investigator-
Indicated
Loadings
6. 3






0.9

0.7

1.0

1.0

1.7

septic tanks)
2. 19xl05
( includes
0.93

0. 9

1.0

                             spring flow)

-------
Table 18 (continued).   US OECD NUTRIENT LOADINGS CALCULATED
         USING WATERSHED NUTRIENT EXPORT COEFFICIENTS

Water Body
Ratio ol Export
Loadings ^ Coefficient
k Calculated Investigator- Loadings to
Point-Source Non-Point Source Loading via Export Indicated Investigator-
Loading3 (g/yr) Coefficients Loadings Indicated
(g/yr) Urban Rural Forest Other (g/m2yr) (g/m2/yr)a Loadings
B. NITROGEN LOADINGS





M
CO
-P








Brownie
(2)
Calhoun
(3)
Canadarago
(5)
Cayuga



Cedar
(7)
Dogfish
George
(12)
Harriet
(13)
Nitrogen

Nitrogen
7.8xl06
B
1.68x10



Nitrogen

Nitrogen
Watershed

Nitrogen

_e
loadings not

loadings not
3-OlxlO6
7
3.66x10



loadings not

loadings not
land usage

loadings not


determined

determined
4.3xl07 l.SlxlO7 1.79xl07 11.8 18.0 0.7
R R R
5.95x10 1.76x10 4.01x10 8.1 14.3 0.6
(does not
include organic
nitrogen)
determined

determined
data not available

determined


-------
Table 18 (continued).   US OECD NUTRIENT LOADINGS CALCULATED
         USING WATERSHED NUTRIENT EXPORT COEFFICIENTS



Water Body
Isles
(14)
Kerr Reser-
voir (Whole
reservoir)
(15)
Lamb
(19)
Meander
(21)
Mendota
(22)




Michigan
Lower Lake
Minnetonka
Potomac
Es tuary




Point-Source Non-Point
Loading3
(g/yr) Urban Rural
Watershed
7
2 . 34x10



0

0

0





Watershed

Watershed
4 x 109
land usage data not
R a
2x10 3x]0



0 0

0 0
p.
7.81x10 2.7x10





land usage data not

land usage data not


Source Loading
(g/yr)
Forest
available
8
1. 2x10


,
1. 6x10
h
1. 34x10
7 U
6.51x10





available

available
1.86xl08 7.46xl08 2.05xlQ8

Loadings
b Calculated
via Export
Coefficients
Other (g/m yr)

7
3.2x10 4.0


u
4x10 0.14
M
3.6x10 0.14
7
1.09x10 1.2
( Includes
groundwater,
baseflow, £
storm
drainage )



9.67xl07 5.4


Investigator-
Indica ted
Loadings
(g/m^/yr )


4. 0



0. 03

0.03

1. 2








5. 0
Ratio of Export
Coefficient
Loadings to
Inves tigator-
Indicated
Loadings


1. 0



4.7

4. 7

1. 0








1.1
(Median flow regime)
(entire estuary)
(27)







-------
Table 18 (continued).   US OECD NUTRIENT LOADINGS CALCULATED
         USING WATERSHED NUTRIENT EXPORT COEFFICIENTS





Water Body
Sallie
(32)
Sammamish
( following
sewage
diversion )
(33)
Shagawa
£ "4)
cn f
Tahoe
(36) f
Twin Lakes
(East Twin
& West Twin
Combined)
1972
(39 g 43)
1973
(40 £ 44)



Point-Source
Loading3
(g/yr)
5. 59x10-
1. 14xl07
Unknown



1.93xl07

0


Lake
Lake

0

0



b
Non-Point Source Loading




(g/yr)
Urban Rural Forest Other
2.25xlO? 3.38xl08 1.04xl08 1.
2.
6.88xl06 l.SxlO6 2.15xl07 4.



8.48xl06 1.3xl06 6.03xl07 1.
2 .
7.2xl07 0 4.72xl07 9.





5.01xl05 0 8.02xl04 5.
r h
5.01x10 0 8.02x10 5.

59x10^
13x10
72xlO?



84xl07-
37x10
04xl08





27xl06
R
02x10


Loadings
Calculated
via Export


Investigator-
Indicated
Coefficients Loadings
(g/m yr) (g/n)2/yr)a
91.6-93.8

-



11.7-12.3

2 . 0





9.6

9.2

2. R-3-0

13. 0



7.8

0. 52





22.6

16.8
(does not
Ratio of Expor
Coefficient
Loadings to
Investigator-
Indicated
Loadings
30-34

-



1.5-1.6

3 .8





0.4

0. 5

                                                  include  organic
                                                    nitrogen)

-------
Table 18 (continued).  US OECD NUTRIENT LOADINGS CALCULATED
         USING WATERSHED IJUTRIENT EXPORT COEFFICIENTS

Ratio of Exporl
Loadings Coefficient
, Calculated Investigator- Loadings to
Point-Source Non-Point Source Loading via Export Indicated Investigator-
Loading9 (g/yr) _ Coefficients Loadings _ Indicated
Water Body
Waldof
(48)
Washington
(assumed 10
urban and 90
forest )
1957
(49)
1964
(50)
1971
(51)
1974
(52)
Weir
(53)

(g/yr)
0


percent
percent

2.01xl08

2. 71xl08

0

0

0


Urban Rural Forest
0 0 5.2xl06





4.02xl07 0 1.45xl08

4.02xl07 0 1.45xl08

4.02x10'' 0 1.45xl08

4.02xl07 0 1.45xl08

1.84xl06 1.68xl07 2.64xl06
( includes
pasture)
Other (g/mzyr)
5.4xl07 2.2





2.07xl08 6.7

2.07xl08 7.5

2.07xl08 4.4

2.07xl08 4.4

5.3xl07 3.1
( includes
septic tanks)
(g/m'/yr) Loadings
0.33 6.6





19. 2 0.3

7.8 1.0

4.6 1.0

4.4 1.0

2.6 1.2



-------
                           Table 18  (continued).   US  OECD NUTRIENT LOADINGS CALCULATED
                                    USING  WATERSHED NUTRIENT EXPORT COEFFICIENTS
h- >
co
            Point-Source
            Loading3
                                          Non-Point  Source  Loading
                                                    (g/yr)
                           Ratio of Export
Loadings                   Coefficient
Calculated   Investigator-Loadings to
via Export   Indicated     Investigator-
Coefficients Loadings      Indicated
Water Body
Wingra
( 54)
(g/yr)
0
Urban Rural Forest Other (g/m yr)
5.24xlOR 0 9.38x10° V.SVxlQ6 9.8
(includes
spring flow)
(g/m2/yr)'
5.1
Loadings
1.9
 Based on investigator's estimates.
b
 Watershed land usage as defined by Uttormark et al.  (1974) and indicated by the investigator.
cAs indicated by the investigator.  Precipitation and dry fallout nutrient inputs,
 if not indicated by the investigator, were calculated using the nutrient coefficients
 given in Sonzogni and Lee (1974).  Other loadings are as indicated in the table.
d                                        '
 Identification number for Figures 15 and 16 (see Table 14 ).

eNitrogen loadings are comprised of inorganic nitrogen (i.e., NHL +N0~+N0~ as N) plus
 organic nitrogen, unless otherwise indicated.
     "low" nitrogen export coefficient of Uttormark e_t al .  (1974) used to determine
 the nitrogen loading estimate.
CT
6Data in parentheses represent data received by these investigators from the principal investigator
 subsequent to the completion of this report.   Figures 15 and 16 are based on the original
 data reported by the investigator and do not  reflect the changes indicated above.  Examination
 of this subsequent data indicates the phosphorus loads were originally underestimated; however,
 there were no significant changes in the overall conclusions concerning the Twin Lakes
 as a result of these altered loads.

-------
these water bodies with those calculated using the nutrient ex-
port coefficients in Table 17 would obviously indicate an error
in the reported nutrient loadings.  Further, it was also felt
by these reviewers that land use export coefficients calculated
for a specific watershed are likely more accurate than the average
values used in these calculations.  Consequently, these water
bodies were not included in Table 18 as it would be incorrect
to check their nutrient loadings in this manner.  Lake Waldo's
reported nutrient loadings are based on an average of several in-
direct methods, including land use export coefficients derived
for the Upper Klamath (Powers et al.,  1975).  However, since more
than one method was used by Powers et al. to calculate Lake Waldo's
nutrient loading and because the value obtained using the export
coefficient was similar to the value obtained with the other in-
direct methods, this water body was retained in Table 18.

     The watershed land use-derived loading estimates for phos-
phorus and nitrogen are compared with the US OECD investigator-
indicated loadings in Figures 15 and 16, respectively.  The
various lines and the shaded zone in Figures 15 and 16 have the
same meaning as those in Figure 14.  Figures 15 and 16 will be
discussed in connection with the Vollenweider loadings diagrams
presented in following sections of this report.

Comparison of Phosphorus Loadings Derived From Vollenwider
Relationship With Loading^ Derived From Watershed Phosphorus
Export Coefficients.

     The phosphorus loadings predicted by Vollenweider's relation-
ship in Equation 26 may be compared with the loadings predicted
with the use of watershed land use phosphorus export coefficients.
If they are similar, one can have some degree of confidence that
their use for determining the correct value for the phosphorus
loadings was somewhat justified.  If they disagree to any major
extent, then one would have to question the use of one or both
of these approaches for predicting the 'correct' phosphorus load-
ings to the US OECD water bodies.  Such a comparison was made
with the US OECD eutrophication study data.  The predicted phos-
phorus loadings, using the Vollenweider relationship expressed
in Equation 26 and the watershed land use phosphorus export co-
efficients, as well as the ratio of the former to the latter, is
presented in Table 19.  The results are presented graphically in
Figure 17.   The various lines and the shaded zone in Figure 17
have the same meaning as in Figure 14.  If a data range was re-
ported for a water body, the mean value was used in all calcula-
tions .

     Examination of Figure 17 shows reasonably good agreement
between the phosphorus loadings predicted for the US OECD water
bodies using the Vollenweider relationship (Equation 26) and
those predicted using watershed phosphorus export coefficients.
Most of the phosphorus loadings predicted using Equation 26 are

                                 139

-------
I   5.0
o
UJ
o
o
    -20X -IOX  -6X
                 -4X
                      -3X
                                -2X
£L
X
UJ
o
Q.
4.0
INVESTIGATOR-INDICATED
   TROPHIC STATE:

  • - EUTROPHIC
  A - MESOTROPHIC
  O - OLIGOTROPHIC
 0            1.0           2.0          3.0
   INVESTIGATOR-INDICATED PHOSPHORUS LOADINGS
                   (gP/m2/yr )
                                                  +3X
                                                  + 6X


                                                  + IOX

                                                  + 20X
 Figure 15.
          Evaluation  of Estimates of  US  OECD
          Water Body  Nutrient  Loadings:
          Watershed  Land Use  Phosphorus
          Coefficient  Calculations
                          140

-------
CO
H
50
40
UJ
a
u.
u.
LJ
O
a
X
LU
2
LL)
O
O
CC
>   30

Q *Z
U  >.

5«
^  E
    -20X -IOX  -6X
                 -4X
                       -3X
                                 -2X
    20
to
C5
z
a
<
O
LJ
O
O
cc
O
     10
     0
                                /
      CALCULATED: 92-94 )
          IN VEST I GATOR'-INDICATED
              TROPIC STATE:/
• - EUTROPHIC/
A - MESOTROPHIC

O- OLIGOTROPHIC
  *


/
                                                Ix
                                                •f3X




                                                44X



                                                +6X



                                                + IOX


                                                +20X
            5      10     15     20    25     30     35

     INVESTIGATOR-INDICATED TOTAL NITROGEN LOADINGS
                       (gN/m2/yr)
Figure  16.
          Evaluation of  Estimates  of  US  OECD
          Water Body Nutrient  Loadings:
          Watershed  Land  Use Nitrogen  Export
          Coefficient Calculations

-------
jr
ro
                            Table  I1'.  COMPARISON  OF  PHOSPHORUS  LOADINGS DERIVED FROM WATER-
                                       SHED  EXPORT COrrFICLENTS  WJTII LOADINGS PREDICTED BY
                                       VOLLENWEIDER'S MEAN  PHOSPHORUS/INFLUENT PHOSPHORUS
                                       AND HYDRAULIT  RESIDENCE  TLMK RELATIONSHIP

Phosphorus Loadings Phosphorus Loadings
Predicted with Vollen- Predicted with Water- Ratio of Vollen-
weider's Relationship shed Phosphorus Ex- weider-Derivcd to
Trophic (Equation 2b)b port Coefficients0 Export Coefficient-
Water Body State3 (g/m^/yr) (g/m2/yr) Derived Loadings
Calhoun ( 3 )d
Canadarago (5)
Cayuga (G)
Cedar (7)
Dogfish (10)
George (12)
Harriet (13)
Isles (14)
Kerr Reservoir (15)
Lamb (19)
Meander (21)
Mendota (22)
Michigan
Open Waters
(23A 6 B)
Lower Lake ~
Minnetonka
Potomac Estuary
(27)
Sal lie (32)
E
E
M
E
0
0-M
E
E
E-M
0
0
E

0


E->M

U-E
E
0.9e
0-9-1.1 1.2 0.8-0.9
0.50 0.9 0.6
0.3e
0 . 0 2S 0.1 o.2
0.07
0.66
0.9e
-
0.05-0.06 0.14 0.4
0.04-0.06 0.14 0.3-0.4
1.2 1.2 !.0

0.1-0.2
~

_ __

5.4
2 .6-4,7 9.4-12.1 U .2-0.5

-------
                         Table 19 (continued).   COMPARISON OF PHOSPHORUS LOADINGS DERIVED FROM WATER-
                                  SHED EXPORT COEFFICIENTS WITH LOADINGS PREDICTED BY VOLLENWEIDER'S
                                  MEAN PHOSPHORUS/INFLUENT PHOSPHORUS AND HYDRAULIC RESIDENCE TIME
                                  RELATIONSHIP
-P
CO

Water Body
Sammamish (33)
Shagawa (34)
Tahoe (36)
Twin Lakes
(East Twin £ West
1972 (39 g 43)
1973 (40 F, 44)
1974 (41 £ 145)
Waldo (48)
Washington
1957 (49)
1964 (50)
1971 (51)
Weir (53)
Wingra (54)
Phosphorus Loadings Phosphorus Loadings
Predicted with Vollen- Predicted with Water- Ratio of Vollen-
weider's Relationship shed Phosphorus Ex- weider-Derived to
Trophic (Equation 25)b port Coefficients0 Export Coefficient-
State3 (g/m^/yr) (g/m^/yr) Derived Loadings
M
E
U-0
Twin)
E
E
E
U-0

E
E
M
M
E
0
0
0
0.7
0. G
0.9
<0

0
2
0
0
0
.4
. 8
.03
-0.9 (0.6)h
-0.9 (0.5)
-1-4 (0.6)
-05g

. 8
. 3
.6
.12
. 7
0
1
0
0
0
0
0

1
1
0
0
0
. 7
.1
.2
. 6 ( 0 . 6 )h
.6(0.6)
•6(0.6)
.12

.1
.6
.45
.24
. 9
0
0
0
1.2
1 .0
1 .5
<0

0
1
1
0
0
.6
.7
. 2
-1.5(1.
-1.5 (0.
-2.3(1.
.4

.7
.4
.3
.5
.8



o)h
8)
0)







            EXPLANATION:

            'Investigator-indicated trophic state:
                 E = eutrophic      0 = oligotrophic
                 M = mesotrophic    U - ultra

-------
             Table 19 (continued).   COMPARISON OF PHOSPHORUS LOADINGS DERIVED FROM WATER-
                      SHED EXPORT COEFFICIENTS WITH LOADINGS PREDICTED BY VOLLENWEIDER1S
                      MEAN PHOSPHORUS/INFLUENT PHOSPHORUS AND HYDRAULIC RESIDENCE TIME
                      RELATIONSHIP
 Phosphorus loadings calculated using the investigator-indicated mean phosphorus concentrations
 and hydraulic loading (Z/T ) data, as applied in Equation 25.

°Phosphorus loadings calculated using the watershed nutrient export coefficients cited in
 Table 17.  Point sources and any other additional nutrient input sources used in the calcula-
 tions were those supplied by the US OECD investigators for their respective water bodies.
 Identification number for Figure 17 (see Table 14).
e                              «
 The mean phosphorus concentrations used in Equation 25 were the average summer surface values.

 Mean phosphorus concentrations were reported for the arms or sub-basins of these water bodies,
 while the watershed land usage patterns were reported for the entire watershed.  Because of
 mixing of nutrients added to the water body as a whole, as well as morphological and hydro-
 logical differences between the sub-basins, it is not possible to calculate phosphorus loadings
 based on watershed land use nutrient export coefficients for these water bodies.
rj
&The mean phosphorus concentrations used in Equation 25 were derived from annual August average
 values.
Y.
 Data in parentheses represent calculations based on data received by these reviewers from
 the principal investigator subsequent to the completion of this report.  Figure 17 is
 based on the original data reported by the investigator and does not reflect the changes
 indicated above.  However, examination of this subsequent data indicated there were no
 significant changes in the overall conclusions concerning the Twin Lakes as a result of
 these altered values.
 Dash (-) indicates data not available.

-------
          -IPX -6X -4X -3X   -2X
                  '  '  /
        UNDERESTIMATION
                   VOLLENWEiDER LOAD)SO s3,4
                 EXPORT COEFFICIENT LOADlNS' 10,8
            INVESTIGATOR / INDICATED
               TROPHIC/STATE:
              • - EUTROPIC
              A - MESOTROPHIC
                                                   + IOX
                               2.0
                              3.0
      PHOSPHORUS  LOADINGS CALCULATED VIA WATERSHED
             PHOSPHORUS EXPORT COEFFICIENTS
Figure  17.
           (g P/mz/yr)

Comparison of Phosphorus  Loadings
Derived  from Watershed Export Co-
efficients with  Loadings  Derived
from Vollenweider  Mean Phosphorus/
Influent  Phosphorus and Hydraulic
Residence  Time Relationship
                                  145

-------
within two-fold of the loadings predicted with nutrient export
coefficients  Given the different components considered in these
two approaches, a phosphorus loading discrepancy of two-fold or
less between these two methods was considered by these reviewers
to be a reasonably good agreement for the water bodies for which
adequate data were available.  The results of Figure 17 and Table
19 will also be discussed in connection with the Vollenweider
loading diagrams presented in subsequent sections of this report,
                                146

-------
                           SECTION VII

           US OECD EUTROPHICATION STUDY PHOSPHORUS DATA:

AS APPLIED IN INITIAL VOLLENWEIDER PHOSPHORUS LOADING AND MEAN
DEPTH/HYDRAULIC RESIDENCE TIME RELATIONSHIP


     With the possible phosphorus loading discrepancies indicated
in the relationships discussed in the previous section (i.e.,
Figures 14-16), it is now appropriate to return to the major
focus of the US OECD eutrophication study and examine the phos-
phorus loading-trophic response relationships in the US OECD
water bodies, as expressed by the Vollenweider phosphorus loading
criteria and other models.

     The Vollenweider diagram of total phosphorus loading and
the ratio of mean depth to hydraulic residence time, as original-
ly developed (Vollenweider, 1975a), containing the US OECD water
bodies for the years for which data were available is presented
in Figure 18.  This is the phosphorus loading diagram which
serves as the basis of the US EPA's Quality Criteria for Water
(US EPA, 1976a) for determining critical phosphorus loads for US
lakes and impoundments.  The pertinent US OECD data are presented
in Table 20.  If a data range was reported for a water body, the
mean value was used in all calculations.  Data were not available
for all water bodies for all time periods.  An example is Dogfish
Lake.  Nutrient data were available only for 1972.  Consequently,
in Figure 18, only Dogfish Lake - 1972 (Identification Number 10)
is presented.  Refer to Table 1M- for identification of any water
bodies and/or time periods not included in a given table or
figure in this report.

     Examination of Figure 18 shows good agreement between the
trophic states of the US OECD eutrophication study water bodies,
as indicated by their position on the Vollenweider phosphorus
loading diagram (based on their reported phosphorus loadings and
mean depth/hydraulic residence time characteristics), and the
trophic states indicated by their principal investigators.  Only
a few water bodies show anomalies between the predicted and re-
ported trophic states.  These anomalies will be discussed shortly.
The small number of US OECD water bodies showing disagreement
between the Vollenweider phosphorus loading diagram-indicated
trophic state associations and the investigator-indicated trophic
states support the validity of the Vollenweider phosphorus load-

                                  147

-------
IV
*-^
N
e
Q.
01
O
Z
Q
O
tn
ir
O O.I
(L
to
O
Q.
0.01
1 ' ' ' 	 ' 1 I 1 1 1 1 1 1 1 1 1 1 1 1 II 1 i (85)l 1 1 1 1 1
I 35 »29 4>
- EUTROPHIC • 128
• 16 w
• 8 •* XX"
m * •' • 5° ^EXCESSIVE
47 l4 *«6 ^^
_ •' *2 54 * * ^^ ^'PERMISSIBLE
~- »I7 • 39», *^ .x--^
I 2^ 3 03£-33 52 ^.-^
^ ^5 ^,^» Af\ Z^l ^^
7 ^ j^^ ^L/ at
-------
                               Table 20.  PHOSPHORUS AND NITROGEN  LOADINGS, MEAN DEPTHS (z)
                                          AND HYDRAULIC RESIDENCE  TIMES (T )  EOR US OECD
                                          WATER BODIES                     "'
-P
CO



Water Body
Blackhawk
Brownie
Calhoun
(l)f
(2)
(3)
Trophic
State*
E
E
E
Mean
Depth ,5
(m)b
4
6
10
.9
.8
.6
Hydraulic
Residence
Time ,T
U)
(yr)°
0
2
3
.5
.0
.6
Total Total
Phosphorus Nitrogen
Loadings Loadings
(£ P/m2
2 .1
1
0
/yr)d (RN/m2/yr)e
-2.3 23.4
.18
.86
Came lot -Sherwood
Complex
Canadarago
Cayuga
Cedar
Cox Hollow
Dogfish
(4)
(5)
(6)
(7)
(8)
(10)
Dutch Hollow(ll)
George
Harriet
Isles
(12)
(13)
(14)
Kerr Reservoir
E
E
M
E
E
0
E
0-M
E
E
E-M
Roanoke Arm(16)
Nutbush Arm(17)
Lamb
(IP)
0
3
7
54
6
3
4
3
18
8
2

10
8
4
0.09-0.14
. 7

.1
.8
.0


.8
.7

.3
.2

0
8
3
.6
.6
.3
0 .5-0.7
3
1
8
2
0

0
5
2
.5
.8
.0
.4
.6

.2
.1
. 3
2.4
0
0
0
1.6
0
1
0
0
2

5
0
0
-2.7 34.6
.8 18
.8 14. 3g
.35
-2.1 19.1
.02
.0 10.4
.07 1.8
.71
.03

.2 36.2
.7 2.4
.03

-------
Table 20  (continued).   PHOSPHORUS AND NITROGEN LOADINGS, MEAN
         DEPTHS (z) AND HYDRAULIC RESIDENCE TIMES (T  ) FOR
         US OECD WATER BODIES.                      "*

Water Body
Meander
Mendota
Trophic
State3
(21)
(22)
Michigan (open waters
1971 (23 A f,
1974
	 p Lower Lake
(j-, Minnetonka
o 1969
1973
Potomac
Upper
Middle
Lower
Redstone
Sallie
Sammamish
Shagawa
(24 A g
(25)
(26)
(28)
(29)
(30)
(31)
(32)
(33)
(34)
0
E
)
B)0
B)0
E
E-+M
U-E
-
-
E
E
M
E
Mean
Depth, z
(m)b
S.O
12
84
84
8.3
8.3
4.8
5.1
7.2
4.3
6.4
18
5.7
Hydraulic
Residence
Time ,T
CM
(yr)c
2 . 7
4.5
30-100
30-100
6.3
6.3
0.04
0.18
0.85
0.7-1.0
1.1-1.8
1.8
0.8
Total Total
Phosphorus Nitrogen
Loadings Loadings
(g P/m2/yr)d (g N/m2/yr)e
0.03
1.2 13
0.14
0.10 1.3
0.5
O.l(0.2)h
85 288
8 32
1.2 2.5
1.4-1.7 18.1
1.5-4.2 2.8-3.0
0.7 13
0.7 7.8

-------
Table 20 (continued).  PHOSPHORUS AND NITROGEN LOADINGS, MEAN
         DEPTHS (z) AND HYDRAULIC RESIDENCE TIMES  (T  )  FOR
         US OECD WATER BODIES.                      w

Water Body
Stewart
Tahoe
East Twin
197?
1973
1974
West Twin
1972
1973
197it
Twin Valley
Virginia
Waldo
Washington
1957
1961)
1971
1974


(35)
(36)

(39)
(40)
(41)

(42)
(43)
(44)
(46)
(47)
(48)

(49)
(50)
(51)
(52)
Trophic
State3
E
U-0

E
E
E

E
E
E
E
E
U-0

E
E
M
M
Mean
Depth , z
< ib
(m)
1
.9
313

5
5
5

4
4
4
3
1







.0
.0
.0

.34
.34
.34
. 0
.7
36

33
33
33
33
Hydraulic
Residence
Time ,T
M
r-
(yr)C
0
.1
700

0
0
0

1
1
1
0 .4
0.9

.8
.9
.5

.6
.8
.0
-0 .5
-2 .8
21

2
2
2
2

.4
.4
.4
.4
Total
Phosphorus
Loadings
(g P/m
4.8
0

0
0
0

0
0
0
1.7
1.2
0

1
2
0
0
2/yr)d (g
-8.
.05

.7
.5
.7

.4
. 3
. 3
-2 .
-1.
0

V.
(0.7)h
(0.5)
(0.8)
V,
(0.4)h
(0.2)
(0.3)
0
5
.017

.2
.3
.43
.47





Total
Nitrogen
Loadings
N/m
73
0

31
19


16
15

17
18
0

19
7
4
4
2/yr)e
.6
.52

.48
.38
-

g
g
-
.4
.3
.33

.2
.8
.6
.4

-------
                              Table 70 (continued).   PHOSPHORUS AND NITROGEN LOADINGS, MEAN
                                       DEPTHS (z) AND HYDRAULIC RESIDENCE TIMES (T ) FOR
                                       US OECD WATER BODIES.                       W
en

Trophic
Water Body State3
Weir (53) M
Wingra (54) E
EXPLANATION:

Mean
Depth, z
(m)b
6. 3
2.4


Hydraulic
Residence
Time ,T
cu
(yr)c
4 .2
O.U


Total
Phosphorus
Loadings
(g P/m2/yr)d
0.14
0.9


Total
Nitrogen
Loadings
(g •N/m2/yr)e
2.6
5.14


                                                         M = mesotrophic
                                                         0 = oligotrophic
                                                         U - ultra

                  Mean depth (?) = water body volume (m )/water body surface area (m ).
                 c                                                    3                         3
                  Hydraulic residence time (f )  = water body vo]ume (m )/annual inflow volume (m /yr).

                  Based on investigator's estimates.
                 eBased on investigator's estimates.  Total nitrogen loading consists of inorganic
                   nitrogen (i.e. , NH^+NO^+NO^-N) + organic nitrogen, unless otherwise indicated.
                 f                   4   J   /
                  Identification number for Figures 18, 19 and 21 (See Table 14)
                 g
                 "Does not include organic nitrogen.

                 1 Data in parentheses represents data received by these reviewers subsequent to the completion
                  of this report.  Figures 18 and 19  are based on the original data supplied by the investi-
                  gators and do not reflect these revised values.  Examination of the data indicated no
                  significant changes in the overall  conclusions concerning these water bodies.

                  Dash (-) indicates data not available.

-------
ing relationship in establishing trophic state associations and
critical phosphorus loading levels for US lakes and impoundments
(i.e., a level which could produce problem algal blooms in water
bodies).

     For the purposes of this section of the report, agreement
or lack of agreement with the Vollenweider relationship is based
on whether the investigator-indicated trophic state is appropriate,
compared with the trophic conditions that Vollenweider and other
US OECD investigators have reported for other water bodies with
similar phosphorus loadings and hydrologic and morphologic
characteristics, (i.e., does a lake designated as eutrophic by
the US OECD investigator hold a position on the Vollenweider load-
ing curve similar to those held by other eutrophic lakes?).
No attempt is being made at this time to further refine this re-
lationship.  If it is completely valid, then lakes with the greater
displacement from the permissible phosphorus loading line should
be more highly eutrophic.  In general, this seems to be the case
for many of the US OECD eutrophication study water bodies.  This
point will be discussed further in a subsequent section of this
report.

AS APPLIED IN MODIFIED VOLLENWEIDER PHOSPHORUS LOADING AND
MEAN DEPTH/HYDRAULIC RESIDENCE TIME RELATIONSHIP

     Vollenweider's modified phosphorus loading and mean
depth/hydraulic residence time diagram is presented with the US
OECD eutrophication study data in Figure 19.  As mentioned in an
earlier section, this modified Vollenweider phosphorus loading
diagram is identical to his original phosphorus loading and mean
depth/hydraulic residence time diagram (Figure 18) except that the
boundary conditions have been altered.  According to Vollenweider
(1975a), these modified boundary conditions are more indicative
of the true phosphorus assimilative capacity of water bodies
than were his original boundary conditions  (Figure 18).  These
altered permissible and excessive loading lines (Figure 19) make
a difference in the trophic zone designation of the loading
diagram, lowering the permissible and excessive phosphorus load-
ing limits for some range of Z/TW values and raising them for other
values of Z/TW.  The original and modified Vollenweider phosphorus
load and mean depth/hydraulic residence time loading diagrams are
superimposed in Figure 20 to illustrate the differences in
trophic zone designations.

     Examination of Figure 20 shows the effect of the modified
boundary conditions is to indicate a lower apparent phosphorus
assimilative capacity (i.e., a lower permissible and excessive
loading line) on the modified loading diagram (Figure 19) for
water bodies with a Z/T^ value of between approximately 2 to 50,
relative to the original Vollenweider phosphorus loading diagram
(Figure 18).  Below a Z/TW value of 2, the phosphorus assimila-
tive capacity becomes constant in the modified Vollenweider phos-
phorus loading diagram.  The excessive and permissible loading
boundary conditions increase in the modified Vollenweider phos-
phorus loading diagram above a z/T^value of about 50.  This

                               153

-------
   I (J pww. j_ _—--y| ..-_..-. yr--j- | f™^^^™"'   V   \  1 1  IT I M     |T«——^MM^^Yn^^^^^HB^HH^Tw^*^l|—p


       EUTROPHIC                      *  "   •     ^/EXCESSIVE  -
a.
31   l
o
z
o
o
o o.i
o
X
CL
 O.OI
                                      >50         /  /PERMISSIBLE
                    47     22   »   30  49
                      "            *  *
                           43
                                            /

                  24-A
                                      /        INVESTIGATOR-INDICATED -
                                  ^y''             TROPHIC STATE '.
                           3-B  ^^                 •-EUTROPHIC
                           £>-•**                     A -MESOTROPHIC
                           O24-B                    O-OLIGOTROPHIC
^                         012
               O
                36
                       I9oo2,
                     1§   48                          OLIGOTROPHIC

                      	i   i  i  i i i 111	i   i  i i  i 11 ii	i   i  iii
    O.I                I               10              IOO            IOOO
             MEAN DEPTH Z/HYDRAULIC RESIDENCE TIME , Tw
                                ( m/yr )
 Figure 19.   US  OECD Data Applied to  Modified Vollenweider
               Phosphorus  Loading  and Mean Depth/Hydraulic
               Residence Time Relationship
                               154

-------
  10
(M
 E

 *  .
 en  '

 O
 Z
 O

 o
 _i

 to

 or
 OQ.I
 OL

 O
 X
 Q.
 .01
                                                III I 1 |     I   I  II II I

                                                        EXCESSIVE

                                                         /
                                                         PERMISSIBLE


                                                          EXCESSIVE


                                                         .^
                                                       • ^PERMISSIBLE
                                                      BOUNDARY CONDITION
                                                      IN INITIAL DIAGRAM.
                                                      (Figure 18)

                                                      BOUNDARY CONDITION"
                                                      IN MODIFIED DIAGRAMl
                                                      (Figure 19)
                                                   ml
   O.I
                     I
10
100
1000
             MEAN  DEPTH. Z/HYDRAULIC RESIDENCE TIME,
                               ( m/yr )
Figure 20.
               Comparison  of Permissible  and  Excessive Loading
               Lines  in Initial  and Modified  Vol1enweider
               Phosphorus  Loading  Diagram.
                              155

-------
increase in phosphorus loading tolerance illustrates the effects
of either a great depth or a very rapid hydraulic flushing time
on increasing the relative phosphorus assimilative capacity of a
water body.  A great depth in a water body usually indicates a
large volume of water, with a likely high degree of dilution of
input nutrients and reduced phosphorus return from the sediments,
and gives the water body a high phosphorus assimilative capacity.
Conversely, a very rapid flushing rate usually indicates that the
nutrients are being washed out of the water body approximately as
rapidly as they are being added to it, giving the water body a
higher phosphorus assimilative capacity than water bodies with a
lower Z/T  value.
         w
     Figure 19 represents one of the major thrusts of the US
OECD eutrophication study.  It demonstrates the relationship
between the phosphorus loadings and trophic conditions of the US
OECD water bodies, as modified by their hydraulic loading, qs.
This is based on their associations on the loading diagram with
water bodies of similar Z/T^ (=qs) characteristics and phosphorus
loads.  It also establishes the permissible and excessive phos-
phorus loading levels for these water bodies.  Figure 19 indicates
that only Lakes Cayuga (6), Lower Minnetonka (26), and Sammamish
(33) have predicted trophic states which are in disagreement with
the trophic state reported by the respective US OECD investigator
(Appendix II).  The results in Figure 19 also provide an indirect
check on the effectiveness of the independent methods (i.e.,
Equation 26 and watershed land use nutrient export coefficients)
used by these reviewers to check on the reasonableness of the
reported US OECD water body phosphorus loadings.  The .anomalies
seen in both the investigator-indicated and phosphorus loading
diagram-derived trophic states in Figure 19, and those seen in
Figures 14 and 15 as related to the results in Figure 19, are dis-
cussed on a water body-by-water body basis in the following sections

     Based on the agreement of the investigator-indicated trophic
states of the US OECD water bodies with the results indicated on
the Vollenweider phosphorus loading diagram (Figure 19), and on
the results of the methods used to check on the reasonableness
of the reported phosphorus loadings (Figures 14 and 15), the
investigator-indicated phosphorus loadings and trophic states of
a majority of the US OECD water bodies appear to be reasonable.
In general, they are indicative of the present trophic condi-
tions of these water bodies.  For the purposes of this report
these reviewers defined a reasonable phosphorus loading to a US
OECD water body as one which is within a factor of two (i.e., +_
two-fold) above or below the phosphorus loadings predicted in
Figures 14 and 15.  There was no technical basis for choosing a
                                156

-------
factor of +_ two to define a reliable phosphorus loading.  A
different value may be as appropriate.  However, Vollenweider
(1977) has indicated that the standard deviation of the relative
error, considering 1/(1 + /r^) as the reference value, corresponds
very well with the +_ 2x assumption.  A lack of agreement between
the calculated and reported phosphorus loads in Figures 14 and
15 could be due either to errors on the part of the investigator
in estimating nutrient loads for the lake, or to different phos-
phorus transport and cycling behavior in the lake's watershed and
in the lake itself than is typically  found for most other lakes.
It should be noted that the implementation of these approaches
(Figures 14 and 15) to check the reported US OECD data has
caused some US OECD eutrophication study investigators to crit-
ically reexamine their nutrient load estimates, resulting in
their finding errors in their original loading estimates.  The
methods presented in this report have been used by these reviewers to
correct for these types of errors.

     The failure of a particular lake or impoundment to fit the
Vollenweider nutrient load-trophic state relationship may also
be due to several other factors in addition to errors  in phos-
phorus loading estimates.  Particularly important would be errors
in estimating hydraulic residence times, as well as personal
biases of the investigators in assigning a particular trophic
state classification to their water body.

     It is very important to also note that a lack of fit of a
particular lake to the Vollenweider total phosphorus load and
mean depth/hydraulic residence time trophic state relationship does
not mean that there have been errors on the part of the investi-
gator in estimating any of these parameters.  It is quite prob-
able that even though Vollenweider and this study have found good
agreement of this relationship for a wide variety of lakes and
impoundments, there will be some water bodies which do not fit this
relationship.  This non-fitting group of lakes and impoundments
would be of particular interest and significance since they would
demonstrate apparently unusual phosphorus utilization.  From the
point of view of water quality management, it is important to
clearly identify water bodies of this type so that appropriate
modifications of the Vollenweider nutrient loading relationship
can be made to any water quality standards that are developed by
water pollution control agencies based on this relationship for
these water bodies.   It is important to note that the Vollenweider
loading diagram is a log-log relationship.  Therefore, small errors
in estimating any of the parameters will not change the position of
a particular water body on the diagram to any large extent.   This
also indicates that a large change in phosphorus loading to a
water body is necessary before a significant change in trophic
state can be expected.

     For example,  consider the possibility that the investigator-
indicated phosphorus loadings to Dutch Hollow (11)  were overestimated


                                157

-------
three-fold in Figure 19.   If one corrected the reported phos-
phorus loading for this error, Dutch Hollow would still be in the
eutrophic zone of the Vollenweider loading diagram.  Using the
same reasoning, the phosphorus loadings to Dogfish (10) could be
increased four-fold, and yet Dogfish would remain in the oligo-
trophic zone of the Vollenweider phosphorus loading diagram.
Therefore, these reviewers examined the investigator-indicated
phosphorus loadings and trophic states for the possibility of an
error if the reported and predicted trophic states of a given water
body were not in agreement in Figure 19 and its reported and pre-
dicted phosphorus loadings were not in agreement in Figures 14-
and 15.

     There were  only  a few  water  bodies which  showed a disagreement
in one or more parameters.  Lake Cayuga (6) and Sammamish (33)
plot with water bodies in the eutrophic zone of the Vollenweider
phosphorus loading diagram (Figure 19).  Yet these two water bodies
were classified as mesotrophic by Oglesby (1975) and Welch et al.
(1975), respectively, on the basis of the structure and pro-
ductivity of their biological communities.  These investigators
felt those factors were more indicative of the true trophic states
of these two water bodies than were their positions on the Vollen-
weider phosphorus loading diagram.  If the investigator-indicated
trophic states of Lakes Cayuga and Sammamish are accurate, then
their positions on the Vollenweider phosphorus loading diagram
(Figure 19) indicate that the Vollenweider relationship between
phosphorus loadings and Z/T^ characteristics does not hold for
Lakes Cayuga and Sammamish, or that the phosphorus loadings in-
dicated by Oglesby (1975) and Welch et_ al.  (1975), respectively,
for these two water bodies may have been overestimated.

     It should be mentioned here that a water body does not abrupt-
ly change in character as soon as it crosses one of the boundary
lines (i.e., permissible  or excessive) in the Vollenweider phos-
phorus loading diagram.  These boundary lines were established
on the basis of a subjective determination between nutrient con-
centration and water quality.   As mentioned in an earlier section
of this report, it would  generally be expected that those water
bodies, with a given mean depth/hydraulic residence time relation-
ship, which have the greater vertical displacement under the per-
missible boundary line on the Vollenweider phosphorus loading dia-
gram (Figure 19) would have the best water quality.  Conversely,
those water bodies of the greater vertical displacement above the
permissible loading line  would have the poorer water quality.
There is a continual gradient of water quality between these two
extremes, with the permissible boundary line defining a general
water quality condition acceptable to the population.

     The possibility of overestimation of the reported phosphorus
loadings for Cayuga (6) and Sammamish (33) is consistent with the
results of Figure 14 for  Lake Cayuga, and with Figure 15 for Lake
Sammamish.   The results of Figure 14 indicate that the reported
phosphorus loadings for Lake Cayuga may have been overestimated

                                158

-------
almost two-fold.  Likewise, the results of Figure 15 indicate the
reported phosphorus loadings for Lake Sammamish may also have been
slightly overestimated.  A reduction of the phosphorus loading
estimates for these two water bodies to the extent indicated in
Figures 14 and 15 would place them closer to the mesotrophic zone
of the Vollenweider loading diagram (Figure 19), more in agree-
ment with their investigator-indicated trophic states.

     One other factor that should be considered in examination of
the US OECD investigator-indicated trophic states for these two
water bodies is that they were established by interpretation of
classical response parameters, specifically their biological
characteristics.  Such interpretation is subjective in nature.
When, for example, does a lake change in character from mesotrophic
to eutrophic?  Thus, the lack of agreement between the predicted
and reported trophic states for these two water bodies could be
attributed to a small error in phosphorus loading estimates, Z/T^
values or the still subjective nature of trophic state classifi-
cation of water bodies.  Oglesby (1977) has also indicated
that, in the case of Lake Cayuga, about 75 percent of the tri-
butary total phosphorus load is adsorbed to soil particles in
the tributary waters.  Only about 5 percent of this adsorbed
phosphorus becomes desorbable in phosphorus free aqueous solution.
Thus, according to Oglesby, a significant portion of the tri-
butary phosphorus load becomes unavailable for phytoplankton
assimilation.  This interpretation is consistent with Lake
Cayuga's lower biological productivity in spite of a phosphorus
load which places it in the eutrophic zone of the Vollenweider
diagram.

     Lower Lake Minnetonka-1973 (26) plots just inside the oligo-
trophic zone on the Vollenweider phosphorus loading diagram
(Figure 19).  However, Megard (1975) classified Lower Lake Minne-
tonka as eutrophic, changing to mesotrophic, suggesting a phosphorus
loading underestimation for this water body.  Sewage effluents,
which was approximately 80 percent of the total phosphorus input,
were diverted from Lower Lake Minnetonka in late 1971-early 1972.
Yet, the eutrophic condition reported for this water body was in-
dicative of Lower Lake Minnetonka in 1973.  This situation is
explainable by the fact that while the phosphorus loadings to
this water body have decreased approximately 80 percent, the
water body has not yet had sufficient time to shift to a new equi-
librium phosphorus concentration.

     Megard (1975) has indicated that Lower Lake Minnetonka appears
to be slowly shifting to a mesotrophic condition, based on its
mean chlorophyll concentrations and Secchi depth measurements.
It is possible, unless other unusual circumstances are present,
the trophic state indicated by its 1973 position in the oligo-
trophic-early mesotrophic zone of the Vollenweider phosphorus
loading diagram (Figure 19) will be indicative of its trophic
state when it has reached a new phosphorus equilibrium condition.


                               159

-------
       It  is  also  possible  that  the  reported  1973  phosphorus
  loadings for  Lower  Lake Minnetonka may  actually  have  been under-
  estimated,  (note:   This predicted  underestimation was subse-
  quently  substantiated  by  Megard  (1977).)   Such  a  possibility  is
  suggested in  Figure 14 based on  the  reported mean phosphorus
  concentrations for  this water  body.   One  of the  necessary para-
  meters needed for Equation  26, which serves as the  basis for
  Figure 14,  is an accurate knowledge  of  the  mean  phosphorus con-
  centration  in the water body.  If  the current  mean  phosphorus
  concentration in Lower Lake Minnetonka  is in a non-equilibrium
  condition,  with  respect to  its phosphorus loading,  because of
  its  recent  remedial treatment, the mean phosphorus  concentration
  in Equation 26 is not  justified.   Its mean  phosphorus concen-
  tration, and  any predicted  phosphorus loading  based  on its
  mean phosphorus  concentration, will  change  with  time  until a new
  steady state  condition is reached  in Lower  Lake  Minnetonka.

      No  watershed land usage data  was available  for Lower Lake
 Minnetonka.   Consequently,  Figure  15  could  not be used to check
 on the reasonableness  of  its 1973  phosphorus loading  estimate.

      Both Lower  Lake Minnetonka and  Lake  Washington have under-
 gone partial  or  total  sewage diversion from the  watershed basin.
 In the past,  it  has been  common practice  to relate the response of
 a water  body  which has undergone nutrient input  reduction to the
 hydraulic residence time, or filling  time (i.e., water body volume
 (nr)/annual inflow volume (m^/yr)) of the water  body.  However, in
 the  case of phosphorus, such an approach  does  not take into con-
 sideration the aqueous chemistry of  phosphorus in its role of
 limiting aquatic plant growth.   It is more realistic  to relate the
 rate of  recovery of a water body,  following nutrient  input re-
 duction,  to the  chemical  residence time of the critical aquatic
 plant limiting nutrient for that water body, rather than to its
 hydraulic residence time.   This approach  in evaluating the re-
 covery of Lake Washington and Lower  Lake  Minnetonka will be dis-
 cussed in a following section.


AS APPLIED IN THE  PHOSPHORUS  RESIDENCE TIME MODEL


      It is generally accepted that  steady  state conditions in a
water body are approached  exponentially in accordance  with the
hydraulic residence time of  the water body.  Assuming  a  lake is a
completely mixed  reactor subjected  to  continual and constant
chemical  influx,  which  only  occurs  through the^outlet, the dynamics
of a  conservative substance  can be  described as:
        V dc/dt = Qci - Qc                            (27)


                                160

-------
                             3
     where V = lake volume (L ),

                                      3 -1
           Q = volumetric flow rate (L T  )

                                                        -3
          c. = influent concentration of substance c (ML  ), and
                                                    _ 3
           c = lake concentration of substance c (ML  ).

Integrating and applying the boundary condition that c=c  at t = o,

                             ,r
         c = c. + (c -c.)e~T/ W                 (28)
              i     o  i


     where T  = V/Q = hydraulic residence time.

This latter equation shows that after a change (increase or de-
crease) in the incoming flux of substance c, steady state condi-
tions are approached exponentially in accordance with the basin's
hydraulic residence time.  According to Rainey (1967) and Vollen-
weider (1969), three hydraulic residence times are required to
reach 95 percent of the new steady state concentrations of sub-
stance c, following a change in the rate of supply of that sub-
stance .

     However, in the case of phosphorus this approach does not
consider the aqueous chemistry of phosphorus as it relates to
limiting aquatic plant growth.  Phosphorus is a non-conservative
substance which undergoes transformations in natural waters.
Accordingly, the recovery of a water body to remedial phosphorus
treatment, whether it involves sewage treatment or diversion, is
more accurately related to the phosphorus chemical residence time
than to the hydraulic residence time.  Once the residence time of
the aquatic plant limiting nutrient (phosphorus or nitrogen) to
a given water body is known, the  rate of the water body's response
to remedial treatment can be predicted if an adequate model is
available.

     One of the frequently-asked  questions in eutrophication
control programs is the rate at which the lake will come to a
new equilibrium condition of water quality after altering the
nutrient input.  There are several deficiencies in Rainey's
approach when it is applied to non-conservative substances, such
as phosphorus.  First, the steady-state lake concentration of phos-
phorus is assumed identical to the influent concentration.  In
reality, annual mean phosphorus concentrations are often lower
than the annual input concentration of phosphorus.  Second,
the lake losses are assumed to occur only through the outlet.
In fact, the major loss of phosphorus in lakes usually occurs as
a result of sedimentation, not outflow discharge.


                              161

-------
     Accordingly, the initial equation (Equation 27) can be mod-
ified to account for these deficiencies.   To account for internal
losses, the expression for phosphorus (P) dynamics becomes
           V dP/dt = QP.  - QP - kPV
                                          (29)
     where K = internal loss rate constant, T
An assumption in this model is that the sedimentation loss is
directly proportional to the mean lake phosphorus content , rather
than to the phosphorus supply.  One other factor that must be con-
sidered is that in stratified lakes, different water layers may
contain different amounts of phosphorus due to biological, chem-
ical and/or physical processes.  An example is the summer growth
period where the phosphorus concentration may only be a fraction of
the whole lake concentration due to algal uptake.  Thus, the out-
wash concentrations may be different during the summer time than
during periods of lesser productivity.  Accordingly, the above
equation may be modified as:
                     V dP/dt = QP. - Q oc p _
                                                (30)
where
                     dimensionless proportionality factor relat-
                     ing annual mean outwash or surface water
                     phosphorus concentration to the mean annual
                     concentration over the whole lake.
     Sonzogni et al .  (1976) have modified this model to predict
changes in the phosphorus concentration as a response to nutrient
input reductions based on the concept of a phosphorus residence
time in natural waters.  Equation 30 can be rearranged as:
                     dP + ((Q
                    + kV)/V) Pdt = (Q/V) Pidt  (31)
Since V/Q = T  , Equation 31 can be simplified as
             to
                     dP + (1/R ) Pdt = (1/T ,) P.dt        (32)
                              p            to   i
           where R  = V/(Q ^ + kV) = phosphorus residence
                  ^                  time in lake

If P = P  at t = 0, Equation 32 can be integrated to produce
          P = P.(R /T )-(P.(R /T )-Pn) e t/Rp
               i  p  to    iptoO       ^
                                                           (33)
The steady state phosphorus concentration is not equal to the in-
put phosphorus concentration, but rather differs by the ratio of
the phosphorus and hydraulic residence times, as
                                162

-------
                     p * = pi(w                      (34>

Thus, the time dependent solution to Equation 33 becomes


                     (P(t)-P
-------
Table 21.  PHOSPHORUS AND NITROGEN RESIDENCE TIMES OF US OECD WATER BODIES

Phosphorus
Mass in
Trophic Water Body
Water Body State* (mg P)
Blackhawk
Brownie
Calhoun
Came ] ot-Sherwood

Canadarago

Cayuga
Cedar
Cox Hollow
Dogfish
Dutch Hollow
George
Harriet
Is]es
Kerr Reservoir
Roanoke Arm
Nutbush Arm
Lamb
Meander
E
E
E
E

E

M
E
E
0
E
0-M
E
E
E-M
_
_
0
0
3

1
2

.71xl08
_
.91xl09
.94xl08
Q
2 .34-2.93x10"

1
2
1
1
6
1
7
1

3
1
1
1
1 1
.84x10
.32xl08
.19xl08
.IGxlO7
.63xl08
.68xl010
.64xl08
.25xl08
-| r\
.71xl010
.23xl010
.92xl07
.62xl07
Phosphorus
Phosphorus Residence
Loading , Time, R
(mg P/yr)D (yr)e p
1.
8
1
6.

6

1
2
6.
5
8.
7
9
8

G
3
1
1
9-2. IxlO9
.59xl07
.46xl09
6-7.5xl09
Q
.0x10
1 1
.36x10
.41xl08
3-8. IxlO8
.8xl06
l-8.6x!08
.7xl09
.94xl08
.53xl08
T -|
.24x10
.5x]010
.21xl07
.OSxlO7
0

1
0

0

1
1
0
2
0
2
0
0

0
0
1
1
.2
_
.3
.04

.4

.4
.0
.2
.0
.8
.2
.8
.1

.06
.4
.6
.5
Inorganic Inorganic
; Hydraulic Nitrogen Inorganic Nitrogen
Residence Mass in Nitrogen Residence
Time , T
(yr)<3 "
0.
2.
3.
0 .09-0

0.

8.
3 .
0.5-0
3 .
1.
8
2.
0 .

0 .
5.
2.
2.
5
0
6
.14

6

6
3
.7
5
8

4
6

2
1
3
7
Water Body Loading Time. R
J (mg N)e (mg N/yr)e (yr)f n
3 .
< 2 .
< 9.
6 .

2.22

3.4
< 2.
8 .
4 .
1.
9 .
< 6.
< 6.

3 .
9.
8.
8.
40xl09
73xl07
91xl08
97xl09
10 11
-2.57x10 1.37x10 0.2
12 12
-4.68x10 2.46x10 1.6
31xl08
89xl08
52xl08
IxlO9
9xl010
78xl08
24xlO?
1 -i
46x10
02xl010
16xl08
IxlO8

-------
                         Table 21  (continued).   PHOSPHORUS AND  NITROGEN RESIDENCE TIKES OF US OECD WATER BODIES
cn
en

Phosphorus
Mass in
Trophic Water Body
Water Body State3
Mendota

Michigan (Open
Water-1974)
Lower Lake
Mirine tonka
1969
1973
Potomac Estuary
Upper Level

Middle Reach

Lower Reach

Redstone
Sallie

Sammamish
Shagawa
Stewart
E


0


E
E-»M

-

_

_

E
E

M
E
E
(mg P)
7.02xlOi0

1 3
6 .33x10


1.30xl010
1.09xl010
i n
8.21x10,"
-3.28x10
1.07x10^
-8.03X1011
l.SlxlO11
-3.02X1011
7.52xl08
1.19xl010

l.OSxlO10
3.15xl09
2.85xl06
Phosphorus
Loading
(mg P/yr)
4 .65x10


5.8x10


1.31x10
2 .62x10

4 .84x10

1.68x10

8 .4x10

10

12



10
9
1 o
-L i

12

11

3 .6-4 .2xl09
7.95x10
-2.23x10
1.4x10
6.44x10
1.2-2x10
9
i n
J-U
10
9
8
Phosphorus
Residence
Time, R
Inorganic Inorganic
Hydraulic Nitrogen Inorganic Nitrogen
Residence Mass in Nitrogen Residence
Time. T Water Body Loading Time. R
/ \ /•* P / \/1LO
(yr)c (yr)a
1





1
4

0

0

0

0
0

0
0
0
. 5


11


.0
.2 (7.0) ^

.04

.2

.3

.2
.8

.8
.5
.02
4


30-


6
6

0

0

0

0 .7
1.1

1
0
0
.5


100


.38
.38

.04

.18

.85

-1.0
-1.8

.8
.8
.08

3


8 .





4
-8
1
-3
2
-7
5
1

6
8
7
(mg N)e li>g«/yrJ=(>
.OxlO11 3.48X1011
(NHj+NOJ-N)
1 U 1 1
28x10 7.54x10


_ _
_
i -i
.92X10,,
.76X1011
.60x10:4
.53X1011
.52xlo}}
.56.10
.97xl09
.49xl010

.48xl010 2.6X1011
.39xl09
.41xl07
\ L
0.9


11


_
_

-



_

_
_

0.2
.
_

-------
Table 21 (continued).  PHOSPHORUS AND NITROGEN RESIDENCE TIMES OF US OECD WATER BODIES

Phosphorus
Mass in
Trophic Water Body
Water Body
Tahoe
East Twin
1972
1973
1974
West Twin
1972
1973
M 1974
CD
cn Twin Valley
Virginia
Waldo
Washington
1957
1964
1971
1974
Weir
Wi ngra
Stated
U-0

E
E
E

E
E
E

E
E
U-0
E
E
M
M
M
E
(mg P)
4 .

1.
1.
1.

1.
1.
1.

1.
2.
4 .
6.
1.
5.

1.
2.
70X1011

OSxlO8
OSxlO8
OSxlO8

77xl08
62xl08
47xl08
0
51xlOB
eoxio7
86xl091
97xl010
92X1011
23X1010
_
21xl010
35xl08
Phosphorus
Loading .
(mg P/yr)D
2 .

1.
1.
1.

1.
1.
1.

1.06
2.07
4 ,
1.
2 .
3 .
4 .
3.
1.
SOxlO10

89xl08
35xl08
89xl08

36xl08
02xl08
02xl08
_
-1 . 25x10
-2.66xl08
59X108
06xlOU
02X1011
76xl010
13X1010
29xl09
2Gxl09
Phosphorus
Residence
Time, R
liydraul ic
Residence
Time, T
(yr)c P (yr)d •»
19

0.
0 .
0.

1.
1.
1.

0.
0.
10
0 .
1.
1.
„
3.
0.


6
8
6

3
6
4

1
1

6 (0.7)3
0 (1.0)
4 (1.3)
(1.4)
7 (24.9)
2
700

0 .8
0.9
0.5

1.6
1.8
1.0

0.4-0.5
0.9-2.8h
21
2 .4
2.4
2 .4
2.4
4.2
0.4
Inorganic Inorganic
Nitrogen Inorganic Nitrogen
Mass in Nitrogen Residence
Water Body Loadinr Time, R^
(mg N)e
3 .13xl012

7.83xl08
1.13xl09
-

1.17xl09
1.22xl09
_
Q
8.58xlOB
6.12xl07
9.72xl091
3.48xlOU
6 .97X1011
5 .23X1011
„
1.06xl010
1.04xl09
(mg N/yr)c (
_

8 .48xl09
5.21xl09
_

5.44X109
S.lOxlO9


_
_
_
2.73X1011
5.65X1011
5.60X1011
_
_
7.20xl09

_

0.1
0.2
-

0.2
0.2
_

-
_
-
1.3 (1.1)
1.2 (0.3)
0.9 (0.3)
- (0.5
- (0.2)
0.1

-------
    Table 21 (continued).   PHOSPHORUS  AND NITROGEN RESIDENCE TIMES OF US OECD WATER BODIES

Phosphorus
Mass in
Trophic Water Body
Water Body State3 (mg P),
EXPLANATION:
a ...
Phosphorus
Loading
(mg P/yr)

Phosphorus
Residence
Time, R
(yr)c p

Inorganic
Hydraulic Nitrogen Inorganic
Residence Mass in Nitrogen
Time, T Water Body Loading
(yr)d u (mg N) (mg N/yr)e

Inorganic
Nitrogen
Residence
Time . R
(yr)f n

= water body volume (m )/annual inflow volume  (m  /yr).
 E = eutrophic, M = mesotrophic,  0 = oligotrophic ,  U = ultra
 Based on investigator's estimates.
°Phosphorus residence time,  R  =  annual mean total  phosphorus content (mg)/annual total
 phosphorus input (mg/yr).
^Hydraulic residence time,
eBased on investigator's estimates;  includes NH^ +  N03 + NQ^ as N, unless otherwise  indicated.
 Inorganic residence time,  R  = annual mean inorganic nitrogen content  (mg)Xannual inorganic
 nitrogen input (mg/yr).
"Hydraulic residence time of whole lake,
 Possible error in hydraulic residence time.
 Mean August value.
 Data in  parentheses represents data received by these reviewers  from the  principal  investigator
 subsequent to completion of this report.  Examination of this data  indicated  no significant
 changes  in the overall conclusions concerning these water  bodies.
 l)rir,h (-) indicates data riot available.

-------
      Lake  Michigan  has  a  hydraulic  residence  time  ranging  from
 30-100  years  (Piwoni  et al.,  1976).   If  it  is assumed  that phos-
 phorus  behaves  as a con'servative  element in Lake Michigan  it
 should  require  approximately  100-300  years  for  Lake  Michigan  to
 reach a new phosphorus  equilibrium  state following reduction  of
 its  phosphorus  loading.  However, the phosphorus residence time,
 based on the  US OECD  data,  is approximately 10  years.   Thus,  the
 phosphorus residence  time model of  Sonzogni et  al. (1976)  predicts
 that it would only  take approximately 30-35 years  to achieve  95
 percent of the  expected change in the phosphorus content in Lake
 Michigan following  a  reduction in its phosphorus loading.


     Megard (1977) has  indicated  that the quantity of phosphorus
in Lower Lake Minnetonka was just  beginning to move toward  a new
equilibrium condition in 1973 because the phosphorus  load was  re-
duced in 1971-1972,  following sewage diversion from the water  body.
He estimated,  on the basis of an  adjusted phosphorus  residence
time  (see below) that a new phosphorus equilibrium would not be
reached until 1979,  approximately  seven years  after diversion,
(Megard, 1975) as compared with the  4.2 years  indicated in  Table
21.  Prior to the sewage diversion,  the phosphorus  residence time
was calculated to be 1.1 years, as compared to one  year in  Table
21.  However,  Megard (1977)  has indicated that the  predicted mean
phosphorus concentration at  the new  equilibrium, based on a
1.1 year residence time, would only  be about 14 yg/1, atypical of
other lakes of the region.  Consequently, he obtained a more con-
servative estimate of 26 yg  P/l at a new equilibrium by adjusting
the new phosphorus residence time  upward from 1.1 to  2.0 years.

     However,  Megard (1977)  has also noted that the 1.1 year
phosphorus residence time in Lower Lake Minnetonka is based on
extensive data and should be considered an accurate estimate.
Since the post diversion phosphorus  load is an estimate of  residual
influx from, non-point sources, it  is necessarily more tenuous  than
the prediversion estimate (Megard, 1977).  Consequently, Megard
suggests the post diversion  phosphorus load estimate  might  be
adjusted up by a factor of 1.8 (i.e., the factor used to adjust
the residence time)  to produce a  post diversion loading of  180
mg P/m /yr, as compared to the 100 mg P/m /yr  reported originally.
Adjusting the load by this 1.8 factor produces the same 26  yg  P/l
mean concentration,  at the new equilibrium, as is obtained  by
increasing the phosphorus residence  time by the -same  factor
(Megard, 1975).   That is,  the computed rate of response would
still be consistent  with the observed response during the first-
two years after diversion.

      Lake  Weir  has  a calculated phosphorus  residence time of
 3.7  years  versus a  reported value of  24.9 years (Table  21).
Messer  (1977) has indicated that, in  addition to the mean depth,
the  flushing  rate,  or hydraulic residence time, is the  princi-
pal  reason  for  the  inverse relationship  between critical phos-

                                  168

-------
phorus load and hydraulic load.  According to Messer, while
this may be true for northern temperate drainage lakes which are
ice-covered during part of the year, Lake Weir is a sub-tropical
seepage lake located in Florida.  While temperate lakes may
lose 10 percent of their hydraulic load through evaporation, Lake
Weir appears to lose about 83 percent of its hydraulic load
due to evaporation (Messer, 1977).  This heavy evaporation loss
is not flushing phosphorus from the lake.  Consequently, Messer
suggests using the hydraulic flushing rate, exclusive of
evaporation, as an estimate of the "effective flushing rate."
For Lake Weir, the hydraulic residence time, exclusive of evapora-
tion, was calculated to be about 24.9 years and indicates the
increased sensitivity of Lake Weir to phosphorus inputs, relative
to non-seepage water bodies.

     Lake Washington provides an example of a lake which has
responded to a decreased nutrient flux.  Table 21 shows that,
based on its hydraulic residence time, Lake Washington would
require about seven to eight years to reach a new phosphorus
equilibrium condition.  However, the response of the lake to
nutrient reduction has been both prompt and sensitive (Edmondson
1970b, 1972).  The lake was considered highly eutrophic in 1964.
Yet, by 1971, following completion of the sewage diversion pro-
ject in the late 1960's, the lake was re-classified as rneso-
trophic by Edmondson (1969, 1970b).  The phosphorus residence
time was calculated as 0.5 years in Table 21.  Consequently,
one would expect a 95 percent recovery of the lake in one to
two years following the sewage diversion.  This situation was
in fact seen in Lake Washington following completion of sewage
diversion in the late 1960's (Edmondson, 1970b; Sonzogni, et al.,
1976).

     Megard (1971) compared the actual rate at which the phos-
phorus concentration in Lake Washington decreased, following
sewage diversion, with the phosphorus concentration predicted
from the phosphorus residence time model.  He found the observed
rates of decrease paralleled the predicted rates, and the
measured phosphorus concentrations were similar to the predicted
phosphorus concentrations.  Based on these results, Lake Washing-
ton provides a successful test of the phosphorus residence time
model as an approach to assessing the rate of recovery of a water
body following phosphorus input reduction.

AS APPLIED IN VOLLENWEIDER EQUATION FOR CRITICAL PHOSPHORUS
LOADING

     In addition to his phosphorus loading diagrams, Vollenweider
(1976a) had derived several equations for calculating the critical
phosphorus loading levels and expected trophic states for lakes
                              169

-------
and impoundments.  As indicated earlier, Equation 19 expresses
a generalized relationship which can be used to determine critical
phosphorus loads for lakes and impoundments, based on their mean
depth and hydraulic residence time characteristics.

     According to Vollenweider (1976a), assuming steady state con-
ditions , water bodies which receive phosphorus loadings below the
critical level defined by Equation 19 would be expected to be in
an oligotrophic condition.  Conversely, water bodies whose phos-
phorus loadings were more than twice the critical loading level
would be expected to be eutrophic.  A water body with phosphorus
loadings between these two limits would be mesotrophic.

     Equation 19 was used by these reviewers to check the reported
phosphorus loading levels and trophic states for the US OECD water
bodies.  The pertinent data for the US OECD water bodies is pre-
sented in Table 22.  If a data range was reported for a water
body, the mean value was used in all calculations.  The last
column in Table 22 indicates the approximate factor by which the
investigator-indicated phosphorus loading exceeds or falls short
of the predicted critical phosphorus loading level predicted by
Equation 19.  For example, Lake Canadargo's reported phosphorus
loading is approximately 3.5 times greater than its calculated
critical phosphorus loading level.  Conversely, Lake Waldo could
adsorb a phosphorus loading increase of over 5.6-fold and still
retain its oligotrophic character, according to Equation 19.
Lake Washington, having a reported phosphorus loading between one
and two times the predicted critical loading, would be classified
as mesotrophic in 1974 on the basis of Equation 19.

     Overall, the results of Table 22 are essentially identical
to those illustrated in the Vollenweider phosphorus loading dia-
gram (Figure 19).  As the investigator-indicated trophic con-
ditions are in good agreement with the trophic states indicated
in Table 22, this lends further support to the use of these two
methodologies for determining the critical phosphorus loads to
water bodies in a variety of trophic conditions.

COMPARISON OF RESULTS

     Before the OECD eutrophication study data can be evaluated
with the Vollenweider phosphorus loading criteria, any discrepan-
cies between the predicted and reported phosphorus loading and
trophic conditions of the US OECD water bodies should be explain-
ed.  This was attempted in previous sections in this report.  It
is also necessary to try to explain why some US OECD water bod-
ies appear to plot accurately on the Vollenweider phosphorus
loading diagram, based on their reported phosphorus loading
and mean depth/hydraulic residence time characteristics and tro-
                                 170

-------
                           Table 22.   US OECD DATA USED IN VOLLENWEIDER'S  CRITICAL  PHOSPHORUS
                                      LOADING EQUATION
h-1
Water Body
Blackhawk
Brownie
Calhoun
Came lot -Sherwood
Canadarago
Cayuga
Cedar
Cox Hollow
Dogfish
Dutch Hollow
George
Har pie t
Isles
Kerr Reservoir
Roanoke Arm
^Jutbush Arm
Hydraulic
Loading, q
(m/yr)a
9.8
3.4
2.94
21.4-33. 3
12.8
6 .28
1 .85
5.4-7.6
1 .14
1.67
2.25
3 .67
4 . 5

51.5
1.G1
Calculated
Critical
Phosphorus
Loading , L^ (P)
(mg P/m2/yr)b
167
82
85
294-433
227
247
52
90-130
33
39
86
94
80

745
52
Calculated
Trophic
State0
E
E
E
E
E
E
E
U-E
0
E
0
E
U-E

E
U-E
Investigator-
Indicated Phos-
phorus Loading
(mg P/m /yr)
2130-2320
1180
860
2350-2 G 80
800
800
350
1G20-2080
20
950-1010
70
710
2030

5200
700
Factor Relating
Investigator- Investigator-
Indicated Indicated Load-
Trophic ing to Calcu-^
State0 'd lated Loading"
E
E
E
E
E
M
E
E
0
E
0-M
E
E

E-M
E-M
+13 to +14
+ 14.4
+ 10.1
+5.4 to f9.1
+ 3.5
+ 3.2
+ 6.7
+12.5 to +21.0
- 1.6
+24 to +25
- 1.2
+ 7.6
+ 25

+ 7.0
+ 14

-------
Table 22 (continued).  US OECD DATA USED IN VOLLENWEIDER'S CRITICAL
         PHOSPHORUS LOADING EQUATION

Water Body
Lamb
Meander
Mendota
Hydraulic
Loading ,q
(m/yr )
1.7U
1.85
2.67
Michigan (open waters)
19711 2.8
t T = 30 yr
1974/ f> 2.8
197lS
0.84
yr
0.8U
Lower Lake Minnetonka
1969 1.32
1973
Potomac Estuary
Upper Reach
Middle Reach
Lower Reach
Redstone
Sal lie
Sammamish
Shagawa
1.32f
120
28.3
8.47
4 .3-6.1
3.56-5 .8?
10
7.1?
Calculated Factor Relating
Critical Investigator- Investigator- Investigator-
Phosphorus Calculated Indicated Phos- Indicated Indicated Load-
Loading ,LC (P) Trophic phorus Loading Trophic ing to Calcu-
(mg P/m2/yr)b Statec (mg P/m2/yr)d State0 'd lated Loading6
44
49
83
181
181
92
92
46
46
1440
403
163
86-1 1 2
83-119
234
135
0
0
U-E
0
0
0
0
E
E
E-
E
E
E
E
E
E
30
30
1200
140
100
140
100
500
100 (180)g
85000
8000
1200
1440-1680
1500-4200
700
700
0
0
E
0
0
0
0
E
E-M
U-E
U-E
U-E
E
E
M
E
- 1.5
- 1.6
+ 14
- 1.3
- 1.8
+ 1.5
+ 1.1
+ 11
+2.2 (+3.9)S
+ 59
+ 20
+ 7.4
+13 to +20
+13 to +51
+ 3.0
+ 5.2

-------
Table 22 (continued).   US OECD DATA USED IN VOLLEHWEIDER' S  CRITICAL
         PHOSPHORUS LOADING EQUATION
Water Body
Stewart
Tahoe
East Twin
1972
1973
1974
West Twin
H 1972
oo 1973
1974
Twin Valley
Virginia
Waldo
Washington
1957
1964
1971
1974
Hydraulic
Loading.q
(m/yr)
23
0

6
5
10

2
2
4
7.6
0.6
1

13
13
13
13
.8
.45

.25
.56


.71
.41
. 34
-9 .5
-0 .9
.71

.8
.8
.8
.8
Calculated
Critical
Phosphorus Calculated
Loading, L (P) Trophic
(mg P/m2/yr)b State0
305
124

118
108
171

61
56
87
130-355
16- 37
95

351
351
351
351
E
0

E
E
E

E
E
M
E
U-E
0

E
E
M
M
Factor Relating
Investigator- Investigator- Investigator-
Indicated Phos- Indicated Indicated Load-
phorus Loading Trophic ing to Calcu-p
.,,2, .d c.d lated Load inn"
(mg P/m /yr) State ^
4820-8050
50

700
500
700

400
300
300
1740-2

(700)g
(500)
(800)

(400)
(200)
(300)
050
1150-1480
17

1200
2300
430
470






E
U-0

E
E
E

E
E
E
E
E
U-0

E
E
M
M
+ 16 to
- 2.5

+ 5
+ 4
+ 4

+ 6
+ 5
+ 3
+ 11 t

.9
.6
.1

.5
.3
.4
o
+ 31 to
- 5

+ 3
+ 6
+ 1
+ 1
.6

.4
.5
.2
. 3
+ 26

( + 5.
(+4.
(+4.

( + 6.
( + 3.
( + 3.
+ 16
+ 92








9)g
6)
7)

5)
6)
4)









-------
                 Table 22 (continued).  US OECD DATA USED IN VOLLENWEIDER'S CRITICAL
                          PHOSPHORUS LOADING EQUATION



Water Body
Weir
Wingra


Hydraulic
Loading ,q
(m/yr)a
1.5
6
Calculated
Critical
Phosphorus
Loading ,Lc(p)
(mg P/m2/yr)b
46
98


Calculated
Trophic
State0
M
E

Investigator-
Indicated Phos-
phorus Loading
(mg P/m2/yr)d
mo
900

Factor Relating
Investigator- Investigator-
Indicated
Trophic
State0 'd
M
E
Indicated Load-
ing to Calcu-
lated Loading6
+ 3.0
+ 9.2
EXPLANATION:


aHydraulic loading, q  = mean depth, z/hydraulic residence time, T .

 Based on Equation 19.

 E = eutrophic, M = mesotrophic, 0 = oligotrophic,  U = ultra

 Based on investigator's estimates.

 Factor by which investigator-indicated loading exceeds (+) or falls  short (-) of
 the critical phosphorus loading predicted by Equation 19.

 Hydraulic residence time for whole lake.
 All data in parentheses represent data received by these reviewers from the principal investigators
 subsequent to completion of this report.  Examination of the data indicates no significant changes
 in the conclusions concerning these water bodies.

-------
phic states, even though other relationships  (Figures 14 or  15)
indicate that the reported phosphorus loadings may be in error.

     This may be partially because the Vollenweider phosphorus
loading diagram is a log-log graph.  This type of graph allows
the values of one or both parameters being plotted to change  con-
siderably without a proportionally large change occurring in  its
position on the graph.  As a result, the reported phosphorus
loadings for many US OECD water bodies can be corrected for  pos-
sible over or underestimations without altering their trophic
state categorizations on the Vollenweider phosphorus loading
diagram (Figure 19).  The only exceptions are those water bod-
ies which plotted near the permissible or excessive boundary
lines.

Discrepancies between Vollenweider Phosphorus Loading Diagram
and Vollenweider Mean" Phosphorus/Infl_ue_n_t Phosphorus And"
Hydraulic Residence Time Diagram

     Figure 14 indicates that the reported phosphorus loading
for several of the US OECD water bodies may have, been under  or
overestimated.  Those water bodies whose reported phosphorus
loadings may be underestimated include Lower  Lake Minnetonka-
1973 (26), East Twin Lake-1974 (41), West Twin Lake-1973 and
1974 (44 and 45, respectively), bake Waldo (48), Lake Weir  (53)
and the Upper Reach of the Potomac Estuary (28).  Conversely, the
phosphorus loadings to Lakes Isles (1.4), the  Roanoke and Nutbush
Arms of the Kerr Reservoir (16 and 17, respectively), Lake Stewart
(35) and Lake Virginia (47) may have bpen overestimated.

     Figure 15, based on watershed land  usage patterns  arid  phos-
phorus  export coefficients, indicates the phosphorus loading
estimates to  Lake Dogfish  (10), Lake  Lamb  (19), Lake Meander (22),
Lake Sallie (32), Lake Tahoe  (36), Lake  Waldo (48) and  Lake
Weir (53) may have  been underestimated.

Lake Wai do --

     Figure 14 indicates that phosphorus  loadings to Lake Waldo
(48) may have been  underestimated bv  three-fold.  Waldo, which  is
classified as ultra-oligot rophi o by Powers  e_t a_l. (1975) falls
in the ultra-oligotrophi r zone of the \/\>l"l enweicfer phosphorus
loading diagram (Figure 19).  If its  phosphorus loading estimates
were corrected to the degree,  indicated  in Figure 14, Lake Waldo
would plot much closer to the meso trophic zone.  However, Its
reported nutrient and chlorophyll concentrations, primary pro-
ductivity and other1 classical trophic state indicators  indicate
that Waldo is ultra-oligotrophic.  It is classed among  the most
pristine lakes in the United  States.  Thus, it would appear  that
the phosphorus loading underestimation indicated in Figure 14 may
be in error, and that the reported phosphorus loading estimate
is correct.

                                175

-------
     There are several possible reasons for the disagreement
between the results of Figure 14 and of the Vollenweider phos-
phorus loading diagram (Figure 19).  The relationship expressed
in Figure 14 (Equation 26) is based partly on the annual mean
phosphorus concentration.  Thus, use of this relationship as a
check on the phosphorus loading to Lake Waldo requires an accurate
knowledge of its annual mean phosphorus concentration.  However,
according to Powers et al. (1975), the mean phosphorus concen-
trations reported for Lake Waldo were determined from an annual
visit to Lake Waldo in August or September from 1969 to 1974.
Thus, the reported mean phosphorus concentration was the August
mean value, rather than the annual mean value, and does not neces-
sarily reflect variations in the mean phospnorus concentrations
over the annual cycle.  It may not be appropriate to apply the re-
ported growing season mean phosphorus concentration for Lake
Waldo to Equation 26 to check on its reported phosphorus loading.
Therefore, the phosphorus loading underestimation for Lake Waldo
in Figure 14 may be incorrect.

    It should also be mentioned that Figure 14 is based on a
relationship derived for phosphorus-limited water bodies.  It
is not clear that phosphorus limits algal growth in Lake Waldo
(Powers et al., 1972; Miller et al., 1974).

    It is possible that the reported phosphorus loading to Waldo
may be in error to some degree.  The phosphorus loadings were not
measured directly.  Rather they were based on the results of four
indirect methods (.Powers et al. , 1975),  The mean phosphorus load-
ing was obtained by averaging the results of these .four methods.
However, the results of these four methods differ by nearly three-
fold.  An average phosphorus loading based on these methods would
incorporate any errors from each method into the final value.
In addition, while Powers et al. (1975) considered the phosphorus
input from precipitation and fallout in their phosphorus loading
estimate, they did not include the phosphorus contribution from
dry fallout (Table 9).  According to Kluesener (1972), Sonzogni
and Lee (1974), Murphy (1974) and Murphy and Doskey (1975), dry
fallout can contribute substantial quantities of phosphorus to
water bodies.  Kluesener (1972) reported dry fallout contributed
about three times as much total phosphorus and twice as much
total nitrogen to Lake Wingra than did precipitation.  Murphy
(1974) reported that dry fallout contributes up to 18 percent
of the present phosphorus loading to Lake Michigan, and that
about half of the dry fallout loading is in the form of ortho-
phosphate, the form most readily available for algal growth.
Thus, this magnitude of phosphorus input could constitute a
significant fraction of the total phosphorus input to oligo-
trophic water bodies, which do not ordinarily have any major
point-source inputs.

    Lake Waldo is still in a pristine state, based on its present
limnological characteristics.  The phosphorus loading could be in-
creased about five-fold, according to both Figure 19 and Table 22,

                                176

-------
without altering its trophic state association in the oligotrophic
category.   However, such an increase in phosphorus loading would
imply a significant decrease in water quality in Lake Waldo.  Its
relatively deep mean depth and long hydraulic residence time, com-
pared to the other US OECD water bodies, implies a relatively
slight increase in phosphorus loading to Waldo could alter its
trophic status.  This view is shared by the US OECD investigator
for Lake Waldo.

Lake Weir--

     The  phosphorus  loading  anomaly  in  Figure 14 concerning  Lake*
Weir may  be more  complicated in nature.  Lake Weir  is  atypical
in  several respects  to  the other  US  OECD water bodies.  It  is
a seepage lake  with  no  natural tributary or  point-source  inputs
of  water  or phosphorus.   Rather,  it  receives its phosphorus  solely
from groundwater  seepage  into the lake, from land runoff  directly
into the  lake  and  from  atmospheric  sources  (i.e., precipitation
and dry fallout)  directly onto its  surface.  Also, it is one  of
only two  US OECD  water  bodies  (Figure  4) located  in a  sub-tropical
 (i.e., warm water)  setting.   According  to  Brezonik  and Messer
 (1975), the application of relationships which were derived  in
temperate zones to  an area of high  permeable sands,  high  soil
temperature, unique  geology  and sub-tropical climate,  as  is  found
in  the Lake Weir  watershed,  is questionable.  It  is possible the
phosphorus loading-algal  response relationships  in  the southern
and southwestern  US  warm-water lakes and impoundments  are dif-
ferent from those  found in north  temperate-cold  water  bodies.
This should be  remembered in examination of  the  phosphorus  load-
ing and trophic characterization  data  for  Lake Weir.

     Figures 14 and  15  indicate the  phosphorus loadings to  Lake
Weir may  have  been  underestimated by a  factor of three.   Table
22  also indicates  the possibility of a  phosphorus loading under-
estimation.  However, Lake Weir plots  in the mesotrophic  zone of
the Vollenweider  phosphorus  loading  diagram, in  agreement with
the trophic condition reported by the  investigators (Brezonik and
Messer, 1975).  A mesotrophic state  is  consistent with the  re-
sults expressed in  Table  22  for Lake Weir.

     If the phosphorus  loading estimates were corrected for  the
three fold underestimation indicated in Figures  14  and 15,
Weir would plot in  the  eutrophic  zone  of the Vollenweider phos-
phorus loading  diagram  (Figure 19).  However, Brezonik and  Messer
(1975) have indicated that while  the concentrations of nitrogen
and phosphorus  are  high throughout the  water column and exceed
Sawyer's  (1947) critical  concentrations at all times of the  year,
primary productivity in Lake Weir is low to  moderate and  nuisance
conditions do  not  occur.  Further, although  macrophytes are  common
in  Lake Weir,  floating  mats  or nuisance growths  of  macrophytes
are not found.  Brezonik  and Messer  also indicated  that generally

                                  177

-------
good water quality is found in Lake Weir.  These indications
suggest the degree of phosphorus loading underestimation
indicated for Lake Weir in Figure 14 may be in error.

     Another possible reason for the disagreement between Figures
14- and 19 may result from a fundamental difference in the phos-
phorus loading-algal response relationships in temperate and sub-
tropical systems.   It is possible that both the reported phos-
phorus loading and trophic state of Lake Weir are correct, and
that what is actually anomalous is the interpretation of the
nutrient loading-algal response relationship in water bodies in
subtropical environments.  A phosphorus loading which would place
a temperate water body in the mesotrophic zone of the Vollenweider
loading diagram may produce trophic conditions in a water body
(with the same mean depth/hydraulic residence time characteristics)
in the sub-tropical setting of Florida which would be interpreted
by most investigators as eutrophic.  Brezonik et a1.  (1969)
have presented some basic differences between northern US
temperate lakes and lakes in north central Florida.  Although
the reported and predicted trophic conditions for Lake Weir are
in agreement in Figure 19, additional research on the nutrient
loading-algal response relationships in warm-water bodies may
still be necessary to determine whether the Vollenweider phos-
phorus loading diagram is applicable in its present form, or
whether the permissible and excessive boundary loading lines
may have to be modified to fit different nutrient loading-algal
response relationships in warm-water lakes and impoundments.


Lower Lake Minnetonka--

     The phosphorus loading to Lower Lake Minnetonka-1973 (26)
is indicated as possibly being underestimated about two-fold in
Figure 14.  Lower Lake Minnetonka plots at the early mesotrophic-
late oligotrophic boundary area of the Vollenweider phosphorus
loading diagram (Figure 19), although Megard (1975) has classified
Minnetonka as eutrophic.  Minnetonka has undergone sewage efflu-
ent diversion, completed in early 1972, reducing the annual phos-
phorus influx almost 80 percent.  Since that time, according to
Megard (1975), a decreasing mean phosphorus concentration and
relative integral photosynthetic rate indicates Lower Lake
Minnetonka to be changing from a eutrophic to a mesotrophic
condition.  This is in agreement with the results of Table 22.
However, the inappropriate use of a non-equilibrium water body
mean phosphorus concentration for predicting phosphorus loading
is likely the reason for the loading underestimation indicated
in Figure 14.  This was discussed in relation with the phosphorus
residence time in a previous section of this report.
                                178

-------
Twin Lakes-1973 and 1974~~

     East Twin Lake-1974 (41) and West Twin Lake-1973 and 1974
(44 and 45, respectively)  are indicated in Figure 14 as possibly
having phosphorus loading underestimations between two and three-
fold.  Based on their plankton characteristics, both East Twin
Lake and West Twin Lake are currently in a eutrophic condition,
according to Cooke et al.  (1975).  These observations are consis-
tent with the trophic cTTaracter for these water bodies predicted
in Table 22 and with the Vollenweider phosphorus loading diagram
(Figure 19).  This suggests the phosphorus loading underestimation
indicated in Figure 14 may be in error.

     As with Lower Lake Minnetonka, the reason for the Twin Lake's
phosphorus loading underestimation indicated in Figure 14 is
likely related to the non-equilibrium mean phosphorus concentra-
tions of these water bodies.   Sewage was diverted from the Twin
Lakes during 1972 to a package plant which discharges away from
the watershed.  Thus, the relationship expressed in Figure 14,
based partly on the mean phosphorus concentration, is likely to
produce erroneous results.

     The phosphorus residence time for Lower Lake Minnetonka is
about four years (Table 21) while that of East Twin and West
Twin is about 1 and 1.5 years, respectively.  Thus, Lower Lake
Minnetonka should reach a new steady-state mean phosphorus con-
centration in about 10 to 12 years.  East Twin Lake and West
Twin Lake should reach their equilibrium states in about three
and five years, respectively.  Thus, while their phosphorus
loadings can be reduced rapidly'to substantially lower levels
by remedial treatments, it will take a longer period of time for
these water bodies to reach new equilibrium mean phosphorus con-
centrations and trophic conditions.  Of the three water bodies,
East Twin Lake appears to be closest to a new equilibrium phos-
phorus concentration, based on its phosphorus residence time.
This is consistent with its position on the Vollenweider phos-
phorus loading diagram (Figure 19) and with the results of
Figure 14.

     One point that should be mentioned here is that, while the
Vollenweider model (Figure 19) appears to accurately predict
the degree of fertility of water bodies as described by their
plankton productivity characteristics, it does not address the
problem of estimation of the degree of fertility expressed in
macrophyte growth.  The Twin Lakes have an extensive littoral
area and approximately half of their primary productivity is
in the form of macrophyte growth.  According to Cooke et al.
(1975), the Twin Lakes are of poorer water quality, from the
point of view of the recreational user, than is indicated by the
early eutrophic characterization given them by the Vollenweider


                                179

-------
phosphorus loading diagram.  The Vollenweider model is based
primarily on plankton characteristics, and may not be applicable
in its present form to water bodies with extensive macrophyte
problems such as are found in the Twin Lakes and several other
US OECD water bodies, or to turbid waters as found in some Texas
lakes and impoundments (Lee, 1974b).

Potomac Estuary and Lake of the Isles--
     The Upper Reach of the Potomac Estuary  (28) is indicated
in Figure 14 to have phosphorus loading underestimations between
two and three fold.  The Potomac Estuary is  indicated by Jaworski
(1975) and on the Vollenweider phosphorus loading diagram
(Figure 19) as being highly eutrophic.  Table 22 also indicates
that the phosphorus loads to all Reaches of  the Potomac Estuary
are all many-fold above the permissible loading levels.  Lake of
the Isles (14) is indicated in Figure 14 as  having a possible
phosphorus loading overestimation of about two fold.  This water
body is characterized by Shapiro (1975a) and on the Vollenweider
phosphorus loading diagram as being highly eutrophic.


     As mentioned earlier,  the relationship  expressed in Figure 14
requires accurate knowledge of the annual mean phosphorus concen-
tration in the water body.   The reported mean phosphorus concen-
trations for the Potomac Estuary and Lake of the Isles were the
mean summer value and the mean summer surface value, respective-
ly, rather than the annual  mean values of these water bodies.
Because these water bodies  are highly eutrophic, the mean phos-
phorus concentration during the summer months will lik-ely vary
cyclically as a function of algal blooms and die-offs.  As a
result, the measured mean phosphorus concentration would be a
function of when the water  body was sampled.   Thus, the use of
the summer mean phosphorus  concentration in  the relationship ex-
pressed in Figure 14 as a check on the phosphorus loading is
probably not valid for these water bodies.

     There are several other eutrophic US OECD water bodies (i.e.,
Brownie, Calhoun, Cedar, Harriet) for which  only the mean summer
phosphorus concentration was reported, yet whose phosphorus load-
ings appear reasonable in Figure 14.  This may be coincidental
as a function of when these water bodies were sampled for their
mean phosphorus concentrations.  These findings are consistent
with the results of Figure  15, which is not  based on mean phos-
phorus concentrations, and  which indicates the phosphorus load-
ings to the Potomac Estuary and Lake of the  Isles to be reason-
able.   One additional factor to consider in  examination of the
Potomac Estuary data is that it has typical  estuarine water circu-
lation patterns.   These circulation patterns would likely alter
the nutrient loading-algal  response relationships which are de-
pendent on hydraulic residence time.
                               180

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Lake Stewart, Lake Virginia and Twin Valley Lake--

     The phosphorus loadings for Twin Valley Lake (46), Lake
Stewart (35) and Lake Virginia (47) are indicated in Figure 14
as being overestimated by approximately two, three and four-fold
respectively.  These water bodies are Wisconsin impoundments
with shallow mean depths and short hydraulic residence times.
According to Piwoni and Lee (1975) and their position on the
Vollenweider phosphorus loading diagram (Figure 19), these water
bodies are highly eutrophic.

     The phosphorus loading underestimation indicated in Figure
14 for Lake Virginia may be due to an error in the calculation
of the hydraulic residence time (i.e., water body volume
(m3)/annual inflow volume (m3/yr)).  With a mean depth of 1.7 m,
and a mean hydraulic residence time of 1.8 years, the resultant
hydraulic loading, qs (Z/TW), calculates to be 0.9 m/yr.  This
value is unrealistically small for Lake Virginia's watershed.
The meteoric discharge rate is a measure of the rate at which
water is supplied to the water body from the watershed.  Accord-
ing to Vollenweider and Dillon (1974; Vollenweider, 1976b), the
relationship is expressed as


               MDR = (qs (A /A ))                    (36)
                           o
        where  MDR = meteoric discharge rate (m/yr),

                q  = hydraulic loading = z/i  (m/yr),


                 z = mean depth (m),

                T  = hydraulic residence time (yr),
                 CO
                                               2
                A  = water body surface area (m ), and
                                      2
                A , = watershed area (m ).
                 d


For Lake Virginia,  MDR = (0.9 m/yr) (1.8 x 105 m2/6.5 x 106 m2)
= 0.02 m/yr.  This low meteoric discharge rate is unlikely for
the Lake Virginia watershed area.   The nearby Dutch Hollow Lake
and Lake Redstone have meteoric discharge rates of 0.22 m/yr
and 0.35 m/yr, respectively.   Since the mean depth, watershed
area and water body surface area appear to be correct for Lake
Virginia, this suggests the hydraulic residence time may be in
error, probably overestimated by a factor of ten.   If the
hydraulic residence	time was  changed from 1.8 to 0.18 years,
the value for [P]/[P] in Figure 14 would change from 0.06 to

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0.6, and the value for 1/(1 + JTp would change from 0.4 to
0.7 (Table 15).   These new values plotted into Figure 14 would
place Lake Virginia in a position corresponding^to less  than a
two-fold phosphorus loading overestimation,  indicating that the
phosphorus loading estimate for Lake Virginia is reasonable.

     Piwoni and Lee (1975) have indicated that the values re-
ported for Lake Virginia are highly uncertain because this water
body is a seepage lake and may behave quite differently from a
water body with a base flow surface input.  They have also
indicated that the phosphorus loading estimates may be high be-
cause of the very sandy soils in Lake Virginia's watershed, which
would reduce overland transport of phosphorus.  This would re-
sult in an indication of a possible phosphorus loading over-
estimation, particularly since the nutrient loadings to Lake
Virginia were estimated from watershed nutrient export coeffi-
cients  (Piwoni and Lee, 1975).  There is also a possibility that
the incoming phosphorus to Lake Virginia may be short-circuited
out of the lake during high flow periods.  This would also pro-
duce a misleading estimate of the phosphorus loadings based on
Equation 26.
      The possible phosphorus loading overestimations for Lake
 Stewart and Twin Valley Lake cannot be resolved in the same man-
 ner.  Their hydraulic residence times appear reasonable, rela-
 tive to the other impoundments in the region.  If Figure 14 is
 incorrect such that the phosphorus loading estimates for Lake
 Stewart and Twin Valley Lake are reasonable, then according to
 Vollenweider (1976a; 1975d) the mean phosphorus concentration
 in these water bodies is lower than would be expected for the
 reported phosphorus loadings.  This indicates that the sedimenta-
 tion rate in these water bodies is statistically above average.
 Such a situation currently exists in Lake Erie (Vollenweider,
 1975d).  Whether this also occurs in Lake Stewart and Twin Valley
 Lake is unknown.

      Another factor which may have to be considered is that the
 reported mean phosphorus concentration in these two water bodies
 is the average of the mean summer and mean winter values.  It
 is not known whether a mean value derived from continuous measure-
 ments over the annual cycle would differ significantly from a mean
 value derived from the summer and winter average value in these
 two water bodies.  A large difference in the value of the mean
 phosphorus concentrations measured by these two methods may
 significantly alter the indicated phosphorus loading overestima-
 tion for Twin Valley Lake and Lake Stewart in Figure 14.  How-
 ever, it should also be noted that' the same procedure was em-
 ployed by Piwoni and Lee (1975) for other US OECD impoundments
 in the same region and Figure 14 indicates the phosphorus loading
 estimates for these other impoundments to be reasonable.  A fac-
 tor which may influence the phosphorus in Lake Stewart compared
 to the other lakes is that a potentially significant part of

                                  182

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Lake Stewart has extensive macrophyte growth which would tend
to alter the cycling of phosphorus in the lake.  Therefore, the
phosphorus loading overestimation indicated for Lake Stewart in
Figure 14 may be incorrect.

Kerr Reservoir--

     Figure 14 indicates the phosphorus loading estimates for
both the Roanoke and Nutbush Arms of the Kerr Reservoir (16 and
17, respectively) may be overestimated between two and four-fold.
The two arms of the Kerr Reservoir have been treated separately
by Weiss and Moore (1975) because they differ significantly in
their morphometric, hydrologic and limnologic characteristics.
In both arms of the reservoir, there is a changing magnitude in
nearly all water quality parameters as one moves from the upstream
end of the arm toward the dam.  In general, the nutrient and
chlorophyll concentrations and associated productivity parameters
decrease as one approaches the dam, indicating a relative increase
in water quality in the direction of the dam.  Weiss (1977)
indicated this shift in water quality illustrates that the
sedimentation characteristics of the upper arms of the Kerr
Reservoir, and probably other river systems impoundments, have a
marked impact on reduction of the phosphorus entering these
water bodies.  The results would be a lower net phosphorus con-
centration in the upper arm than expected (this was discussed
earlier in relation to the inorganic nitrogen:soluble ortho-
phosphate ratio in the Kerr Reservoir; see Tables 9 and 10).
When this lower phosphorus concentration was inserted into
Equation 25 the result was the predicted underestimation of
phosphorus load indicated in Figure 14.  Weiss (1977) rioted that
this interpretation was substantiated by Table 18, in which the
phosphorus load prediction is based on watershed phosphorus
export coefficients.

     The flushing rate is believed to be the major controlling
variable in establishing the relative degree of fertility and
behavior differences in the two arms.  According to Weiss and
Moore (1975) the hydraulic residence time is approximately
70 days in the Roanoke Arm and approximately 1800 days in the
Nutbush Arm.  These computations are based on inflow water volume
and do not consider exchange of water between the main body of
the lake and the arms.  The actual hydraulic residence time of
the water in each arm would likely be less than the indicated
amount by a factor somewhat proportional to water exchange
between various parts of the lake.  However, Weiss (1977) has
indicated that the main flow of water through the Kerr Reser-
voir is down the Roanoke Arm and into the major basin above the
dam.  The hydraulic load down the Roanoke Arm is so much faster
than the flow from the Nutbush Arm that exchange of water be-
tween the two arms is inconsequential.  Weiss has indicated that
this is substantiated by the fact that the phosphorus concen-

                                183

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tration at the end of the Nutbush and Roanoke Arms, where they
both enter the main basin, are approximately the same,
suggesting that interchange effects are negligible.  The high
correlation of growth parameters with the hydraulic residence
time indicates the importance of this factor in establishing the
relative degree of fertility of the two arms.


      The two arms of the Kerr Reservoir are described as
 eutrophic-mesotrophic by Weiss and Moore (1975)  and plot in the
 eutrophic zone on the Vollenweider phosphorus loading diagram
 (Figure 19).   The two arms would still remain in the eutrophic
 zone  of the  Vollenweider loading diagram if their phosphorus
 loading estimates were reduced by the  degree indicated in
 Figure  14.   However,  they would be closer to the excessive  load-
 ing boundary line.   Unfortunately, watershed land usage data
 was available only for the whole watershed,  not  for the sub-
 watersheds of the two arms.   Since the amount of mixing between
 the two arms could not be estimated, it was  not  possible to use
 Figure  15 to check on the reported phosphorus loadings.   How-
 ever,  Table  22 indicates that the phosphorus loadings are many
 fold  above the permissible level.   While it  is not unequivocal,
 this  implies the  phosphorus  loading overestimation indicated
 in  Figure 14  for  the  two arms of the Kerr Reservoir may be  in-
 correct .
Discrepancies Between Vollenweider Phosphorus Loading Diagram
and Watershed Phosphorus Export Coefficient Calculations

Dogfish Lake, Lamb Lake and Meander Lake—

     Figure 15 indicates the phosphorus loadings for Lakes Dog-
fish (10), Lamb  (19) and Meander  (21) are approximately five-
fold underestimated.  Contrastingly, Figure 14 indicates their
phosphorus loadings are reasonable.  The results of Table  22
are consistent with the phosphorus loading underestimation in-
dicated in Figure 15.  Thus, it would appear that the reported
phosphorus loadings and the ultra-oligotrophic conditions of
Dogfish, Lamb and Meander predicted in Figure 19 may be in
error.  The_low  chlorophyll level in these water bodies indicate:;
them to be in relatively unproductive states.  However, accord-
ing to Table  22, they are not in the ultra-oligotrophic state
indicated by their large vertical distance below the permissible
                                 184

-------
loading line on the Vollenweider phosphorus loading diagram
(Figure 19).  Based on their phosphorus and hydraulic loadings,
these three water bodies plot in the same general area of the
Vollenweider phosphorus loading diagram (Figure 19) as does Lake
Waldo, implying that they exhibit about the same relative degree
of oligotrophy as does pristine Lake Waldo.  However, their water
quality does not support the view that they are relatively as
oligotrophic as Lake Waldo.  The reported mean phosphorus and
nitrogen concentrations are all higher in Lakes Dogfish, Lamb
and Meander than those reported for Lake Waldo.  Further, the
mean chlorophyll concentrations are also considerably higher in
Dogfish, Lamb and Meander than in Waldo, in some instances by an
order of magnitude or greater.  Secchi depth is also considerably
greater in Waldo than in Dogfish, Lamb and Meander.  However,
these three water bodies are reported to have high humic color
and, therefore, possibly have reduced light penetration.  Con-
sequently, comparison of Secchi depth measurements would not
yield reliable information concerning the degree of oligotrophy
in Dogfish, Lamb and Meander relative to Waldo.  It should also
be mentioned that the higher chlorophyll concentration in Dog-
fish, Lamb and Meander than that found in Waldo implies the
color of the water is not reducing the primary production in
these three water bodies to any great extent relative to Waldo.

     In general, the results of Figure 15, Table 22 and the
reported water quality data Indicate that the reported phos-
phorus loadings for Lakes Dogfish, Lamb and Meander may have
been underestimated, though perhaps not to the extent indicated
in Figure 15.  Consequently, their position on the Vollenweider
phosphorus loading diagram may have to be adjusted accordingly
so as to produce an accurate representation of the relative
trophic states of these three water bodies.

Lake Tahoe--

     Figure 15 indicates the phosphorus loading to Lake Tahoe  (36)
may have been overestimated by a factor of four.  However, Lake
Tahoe appears to be nitrogen-limited with respect to aquatic
plant nutrient requirements (Table 9).  As the Vollenweider
phosphorus loading diagram was developed for phosphorus-limited
water bodies, attempting to categorize its trophic condition based
solely on its trophic state association in the Vollenweider phos-
phorus loading diagram may not be a valid procedure.  Therefore,
Lake Tahoe's nutrient loading-trophic response relationship will
be examined further in an analysis of the US OECD water body
nitrogen-loading estimates in a subsequent section.  It should
be noted that Schindler (1977) has recently indicated there ap-
pears to exist a very precise relationship between the total
phosphorus concentration in a water body and the standing crop
                                185

-------
of phytoplankton, even In water bodies whose low N:P ratios should
favor nitrogen limitation.   This suggests that natural mechanisms
may compensate for deficiencies of nitrogen in many water bodies.

Lake Sallie--

     Figure 15 indicates Lake Sallie's (32) phosphorus loadings
may have been underestimated between two to seven fold.  The same
trend is noted in Figure 14.  Lake Sallie possesses one of the
highest ratios of watershed area to water body surface areas of
all the US OECD water bodies.  Thus, its phosphorus loading is
very high when it is calculated with watershed land use phosphorus
coefficients.  Lake Sallie plots in the ultra-eutrophic zone.
However, Neel (1975) characterizes Lake Sallie as being in a
late mesotrophic-early eutrophic state, suggesting the high de-
gree of fertility indicated in Figure 19 may be in error.  Accord-
ing to Neel, the atmospheric input of phosphorus from dry fallout
was not considered in the phosphorus loading estimates.  There-
fore, it is  possible  that  Lake  Sallie's  phosphorus  loadings  are
underestimated to some degree.  Table 22 also indicates that Lake
Sallie may be more fertile than the investigator-indicated late
mesotrophic-early eutrophic condition.

     However, one other factor that must be considered is that the
water quality problems associated with excessive nutrients in Lake
Sallie are manifested to a major extent in the growth of attached
macrophytes.  As discussed in earlier sections of this report,
the excessive and permissible loading lines on the Vollenweider
phosphorus loading diagram (Figure 19) are based primarily on
planktonic algal problems and may not be applicable to water
bodies such as Lake Sallie which possess extensive beds of macro-
phytes .  The relatively high phosphorus loading to Lake Sallie
may be assimilated to a great extent in macrophyte growth, rather
than by algal uptake.  This would keep both the algal and mean
phosphorus concentrations in Lake Sallie lower than expected from
its reported phosphorus loading.  This would explain why Figure
15, based on watershed land usage, indicates a possible phosphorus
loading underestimation for Lake Sallie while Figure 14, based
partly on mean phosphorus concentration, indicates the phosphorus
loading to be reasonable.  Any estimation of trophic state, based
on Lake Sallie's algal characteristics alone, would likely indicate
a trophic condition which is consistent with that indicated by
Neel (1975), but which is not a realistic appraisal of the over-
all degree of the fertility of Lake Sallie because it ignores the
portion of Lake Sallie's primary productivity which is manifested
in macrophyte growth.
                                186

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                          SECTION VIII

             US OECD EUTROPHICATION STUDY NITROGEN DATA:
AS APPLIED IN VOLLENWEIDER NITROGEN LOADING AND MEAN
DEPTH/HYDRAULIC RESIDENCE TIME RELATIONSHIP

     In addition to phosphorus loadings, the Vollenweider relation-
ship can also be applied to total nitrogen loadings.  However,
because of the relatively scant knowledge concerning nitrogen
relationships in natural waters, Vollenweider has not developed
the permissible and excessive boundary conditions for a nitrogen
loading-mean depth/hydraulic residence time relationship.  Thus,
the trophic state of a water body which is nitrogen limited with
respect to aquatic plant nutrient requirements cannot be deter-
mined in the same manner as with Vollenweider's phosphorus load-
ing diagram.  Conceptually, such an application is possible.
However, it would necessarily be more difficult to establish the
permissible and excessive nitrogen loading boundary lines on
such a loading diagram.

     As indicated earlier, several approaches could be utilized
to develop critical nitrogen loadings for lakes.   One of the most
obvious involves using a direct proportion between the critical
N and P loadings based on typical algal stoichiometry of 16
nitrogen atoms for every phosphorus atom.  On a mass basis, this
would mean that the permissible nitrogen loadings would be in-
creased by approximately 7.5 times the corresponding phosphorus
loadings.

     Another approach would be utilization of the equivalent
nitrogen concentrations developed by Sawyer (1947).  The validity
for this approach stems from the fact that Sawyer's critical
phosphorus concentrations play a dominant role in establishing
the permissible and excessive lines on the Vollenweider phosphorus
loading relationship.  Sawyer suggested a critical inorganic
nitrogen concentration of 0.3 mg N/l.  There are a number of
potential problems involved in attempting to use a direct pro-
portion between nitrogen and phosphorus critical loads, the most
important of which would occur in highly eutrophic lakes, where
nitrogen,  rather than phosphorus, is frequently the key limiting
element.  In these water bodies, blue-green algae, some of which
are nitrogen fixers, often dominate.  While nitrogen fixation
does occur in many lakes, its overall significance is poorly

                                187

-------
understood.  It does not appear, as sometimes stated, that
nitrogen fixation prevents lakes from becoming nitrogen limited.
There are some lakes which show significant nitrogen limitation
in the presence of nitrogen-fixing algae.  Torrey and Lee (1976),
studying Lake Mendota, found that less than 10 percent of the
total nitrogen input was from nitrogen fixation.

     Eutrophic lakes frequently show appreciable denitrification
reactions in which nitrate is converted to nitrogen gas in
anoxic waters and sediments.  This type of reaction would tend
to convert readily available nitrogen into unavailable forms.
Brezonik and Lee (1968) determined the significance of denitrifi-
cation as a means of removing nitrogen from Lake Mendota.

     Probably one of the most significant problems with trying to
develop a similar set of relationships for nitrogen as have been
presented for phosphorus is that it is often more difficult to
accurately estimate nitrogen loads.  Potentially significant
problems occur with estimations of nitrogen input from ground-
water, which can be an appreciable nitrogen source for some lakes.
As discussed by Sonzogni and Lee (1974), even if the groundwater
input and its associated nitrate content are known, one cannot be
certain of the degree of nitrification, if any, that will occur
when the groundwater nitrate comes in contact with the lake
sediments.

     The total nitrogen loading diagram, containing the data for
the US OECD water bodies, is presented in Figure 21.  The data
was presented in Table 20.  The total nitrogen loadings is com-+
prised of the inorganic nitrogen fraction (i.e., _N03 + N02 + NHij.
as N), plus the organic nitrogen fraction, except as indicated.
There are fewer data points in Figure 21 than in Figure 19
because nitrogen loadings were not reported for all the US OECD
water bodies.

     If one compares the nitrogen loading diagram (Figure 21)
with the phosphorus loading diagram (Figure 19), an interesting
observation is that, except for the order of magnitude difference
on the loading axis, there is a good agreement between the
relative positions of the common water bodies on both the load-
ing diagrams.  The relative zones denoting the different trophic
states on the phosphorus loading diagram are also maintained on
the nitrogen loading diagram.  This similarity implies that a
water body receives nutrients in a relatively constant ratio,
with the nitrogen loading being approximately one order of
magnitude greater than the phosphorus loading.  This is consistent
with the view that different types of land usage within a watershed
will yield a relatively constant amount of nutrient export over
the annual cycle.  In addition, the ratio of nitrogen to phosphorus
of ten to one is approximately at the boundary condition between
limiting nutrients (i.e., above an N:P mass ratio of about eight
to one, phosphorus is the limiting aquatic plant nutrient; below
an eight to one ratio, nitrogen appears to be the limiting

                                 188

-------
  100
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tr
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   10
                                            '35
                                       :(288)



                                       28
                                    .39
                           t29  *I6
                      47

                                 SU-^S
                                  40
                                       46 5
                          Jl  W22
                                      34  -50
                                    (54
                      I5'
                       52
                         .53
                                  .32
                        17'
                                      •30
        O
 O
23-B
 12

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23-A
                ,36
       48
          I   I   I
                     III
                  III
INVESTIGATOR- INDICATED

   TROPHIC  STATE".


    • -EUTROPHIC



    A- MESOTROPHIC


    O-OLIGOTROPHIC
                                                                   I  I I
     01                I                 10                100


             MEAN  DEPTH,Z/HYDRAULIC RESIDENCE TIME,TW


                                 (m /yr)
                                                    1000
Figure 21.   US  OECD  Data Applied  to Vollenweider Nitrogen

              Loading  and Mean  Depth/Hydraulic  Residence

              Time Relationship
                                   189

-------
nutrient -- see Tables 9 and 10). This implies nitrogen and
phosphorus are present in such constant relative amounts that
either nutrient could become limiting with a small relative
increase in the other.  Such a view is consistent with a water
body being phosphorus-limited during one time of the year and
nitrogen-limited during another time of the year (i.e., Lake-
Mendota).  It is also consistent with nitrogen limitation in one
portion of a water body and phosphorus limitation in another portion
of the same water body at the same time because of different land
usage patterns in different portions of the watershed (i.e.,
Potomac Estuary -- see Table 9).

     There is no equivalent expression for Vollenweider's mean
phosphorus/influent phosphorus concentration relationship
(Equation 26) to check the US OECD nitrogen loading estimates.
There is also no equivalent expression for Vollenweider's criti-
cal phosphorus loading relationship (Equation 19) which can be
applied to the US OECD water body loadings.  However, it is
possible to compare the reported nitrogen loadings with those
predicted with the watershed land use nitrogen export coefficient
calculations.  This was done earlier for the US OECD water
bodies (Figure 16).  The US OECD data were presented in Table 18.
The nitrogen watershed land use export and atmospheric input
coefficients used by these reviewers were taken from Table 17 .

     Figure 16 indicates generally good agreement between the
predicted and reported nitrogen loadings for the US OECD water
bodies.  As with the phosphorus loadings, a nitrogen loading
was considered reasonable if it was within two-fold above or
below the nitrogen loading predicted with the use of the water-
shed land use nitrogen export calculations.  However, it should
be noted that most US OECD investigators did not report data for
dry fallout and nitrogen fixation in their nitrogen inputs
(Table 13). If the results of Figure 16 are correct, this
suggests these sources are not significant nitrogen inputs to
the US OECD water bodies when they are compared to the other
nitrogen inputs.  This is inconsistent with the observations of
Kluesener (1972) and Sonzogni and Lee (1974) who reported that
nitrogen inputs from these two sources could be substantial.

COMPARISON OF RESULTS:

Discrepancies Between Investigator-Indicated Nitrogen Loadings
and Watershed Nitrogen Export^ Coefficient Calculations

     There are a few US OECD water bodies in Figure 16 whose
reported nitrogen loadings are indicated as possibly being in
error.  These include Lake Sallie (32), Lake Sammamish (33),
Lake Tahoe (36), East Twin Lake-1972 (39), West Twin Lake-
1972 (43), and Lake Waldo (48).  Among the US OECD water bodies
whose nitrogen loadings are indicated in Figure 16 as possibly
being in error, only Lakes Sallie (32), Tahoe (36) and Waldo
(48) may be nitrogen-limited.

                               190

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Lake Sallie --

     Lake Sallie is indicated as having a nitrogen loading under-
estimation of approximately thirty-fold.  In Vollenweider's
phosphorus loading diagram (Figure 19), Lake Sallie .plots in a
zone indicative of a relatively advanced eutrophic condition.
However, as Lake Sallie may not be phosphorus-limited (Table 9),
this predicted trophic condition in Figure 19 may not be indica-
tive of Lake Sallie's true trophic state.  In fact, Neel (1975)
has characterized Lake Sallie as being in a late mesotrophic-
early eutrophic condition.  Neel (1975) has also indicated that
phosphorus does not appear to control algal growth in Lake Sallie
beyond a certain point.  This is consistent with observations
made by Vollenweider (1975a) that as a water body becomes more
eutrophic, beyond a certain point nitrogen becomes the limiting
nutrient, even though phosphorus may initially have been limit-
ing aquatic plant growth.  According to Vollenweider, the turn-
ing point is reached when the ratio of the nitrogen residence
time to the phosphorus residence time drops below a value of one.
However, only the inorganic nitrogen concentration for Lake
Sallie was reported.   Calculation of the nitrogen residence time
requires the total (i.e., organic fraction + inorganic fraction)
nitrogen concentration be known.  Therefore, calculation of the
ratio of the residence times of nitrogen to phosphorus is not
possible for Lake Sallie (see Table 21).  As a result, it is not
clear whether nitrogen or phosphorus limits algal growth in Lake
Sallie.

Lake Tahoe --

     The nitrogen loading estimate for Lake Tahoe (36) is
indicated in Figure 16 as being underestimated about four-fold.
This water body is classified as ultra-oligotrophic by Goldman
(1975) and by its position on the Vollenweider phosphorus load-
ing diagram (Figure 19).  It also plots in the lower half of
the nitrogen loading diagram (Figure 21), implying an oligotrophic
status.  Lake Tahoe is nitrogen-limited (Table 9) according to
its investigator.

     The atmospheric nitrogen contributions for Lake Tahoe were
considered insignificant by Goldman (1975).  However, several
investigators (Kluesener, 1972; Sonzogni and Lee, 1974; Murphy,
1974) have indicated this can be a significant nutrient source,
especially for oligotrophic water bodies.  In addition, the
nitrogen contribution from nitrogen fixation was not considered
in the nitrogen loading estimate for Lake Tahoe, though this
latter source is likely small.

     The present condition of Lake Tahoe indicates it to be much
closer to its limit of permissible nutrient loading than
originally thought (Vollenweider and Dillon, 1974).  Thus, the
nitrogen loadings to Lake Tahoe may have been underestimated to
some degree.   However, it is not clear that the reported nitrogen

                                191

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loadings have been underestimated by the factor of four indicated
in Figure 16.

Lake Sammamish. Lake Cavuga and Twin Lakes --

     Lake Sammamish, East Twin Lake-1972 and West Twin Lake-1972
show apparent nitrogen loading overestimations based on Figure 16.
Dry fallout and nitrogen fixation contributions were not considered
in the nitrogen loading estimates for these water bodies.   As a
result, one would expect the nitrogen loadings to be underestimated,
rather than overestimated, unless the nitrogen loadings from one
or more of the sources have been highly overestimated.  The pos-
sible nitrogen loading overestimations of approximately two-fold
for the Twin Lakes (East Twin Lake-1972 (39) and 1974 (40) and
West Twin Lake-1972 (43) and 1973 (44)) indicated in Figure 16
are likely in error.  The nitrogen loading for Cayuga (6)  is also
possibly overestimated by nearly two-fold.  The nitrogen loadings
reported for these three water bodies comprise only the inorganic
nitrogen fractions of the total nitrogen loading.  They do not
include the organic nitrogen fraction.  While the organic  nitrogen
fraction is not immediately available for algal growth, Cowen et al.
(1976a; 1976b) have reported that, under optimal conditions, 50~to~
80 percent of the organic nitrogen fraction present in urban and
rural runoff can be converted, in a few weeks to several months,
to inorganic nitrogen forms available for algal growth.  Conse-
quently, omission of the organic nitrogen fraction can result in
a gross underestimation of the total nitrogen loading to a water
body in an urban or rural area.  It would seem that these  three
water bodies could not exhibit the nitrogen loading overestimation
indicated in Figure 16 unless the inorganic nitrogen 'fraction of
the total nitrogen loading has been grossly overestimated.  As
a result, the overestimation of the nitrogen loadings indicated
in Figure 16 for the Twin Lakes and Lake Cayuga may be in  error.

     In general, the nitrogen loadings for most of the US  OECD
water bodies, when compared with the nitrogen loadings derived
from watershed land use nitrogen export coefficients, appear to
be reasonable.  This supports the view of these reviewers  that
the use of a nitrogen loading diagram for denoting trophic state
associations for nitrogen-limited water bodies, similar to the
Vollenweider phosphorus loading diagram for phosphorus-limited
water bodies (i.e., Figure 19), is plausible.  Such an applica-
tion, however, must wait until a valid input-output model
similar to that derived for phosphorus (Vollenweider, 1975a)
is available for nitrogen loadings.
                                192

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                           SECTION IX

       US OECD DATA APPLIED IN OTHER NUTRIENT RELATIONSHIPS
US OECD PHOSPHORUS DATA APPLIED IN VOLLENWEIDER ' S PHOSPHORUS
LOADING CHARACTERISTICS AND MEAN CHLOROPHYLL RELATIONSHIP

     As indicated earlier, several investigators have demonstrated
a relationship between phosphorus concentration at spring over-
turn and the  annual  or  summer  chlorophyll  concentrations  (Sawyer,
1947; Sakamoto, 1966; Dillon, 1974a; Dillon and Rigler, 1974a;
Jones and Bachmann , 1976).  A positive correlation between these
parameters was also illustrated by Vollenweider at the May, 1975
North American OECD meeting in Minneapolis.  Consequently, Vollen-
weider (1976a) developed a diagram for predicting algal biomass,
expressed as chlorophyll concentration, as a function of a water
body's specific phosphorus loading characteristics.  The deriva-
tion of this approach was presented in an earlier section of this
report (see Equation 20 and Figure 11).  The reader is reminded
that this phosphorus loading expression (L(P) /qs) / (l+/z7q^) is
equivalent to the predicted in-lake steady state mean phosphorus
concentration.  In Equation 20 (used in Figure 22), the phosphorus
loadings can be checked as a function of the term L(P)/qs and
related to the mean in-lake phosphorus concentration.  A similar
approach was used to check the phosphorus loading estimates, as
illustrated in Figure I'l and Equations 25 and 26.

     The phosphorus loading characteristics and epilimnetic mean
chlorophyll a diagram is presented in  Figure 22 for the US OECD
water bodies.  The pertinent data for  this diagram are presented
in Table 23.  If a data range was reported for a water body, the
mean value was used in all calculations.
      Based on Sawyer's (1947) and Sakamoto's (1966) critical nu-
 trient concentrations, oligotrophic water bodies will plot to the
 left of the 10 mg/rn^ phosphorus loading characteristics level, and
 eutrophic water bodies to the right of the 20 mg/m  phosphorus
 Io-.idi.ng char -soter'istirs level. The mesotrophic water bodies would
 plot between these two loading levels.  The relative degree of
 •--HI trophy or oligotrophy of a water body is determined by its hori-
 zontal displacement to the right or left of the 10 mg/m^ phos-
 phorus loading characteristics level (i.e., predicted in-lake
 steady state phosphorus concentration).  Thus, this 10 mg/m^ con-
 centration corresponds to Vollenweider ' s (Figure 19) permissible
 phosphorus loading.
                                 193

-------
                                   Table  23.   US  OECn  DATA APPLIED TO VOLLENWEIDER'S  PHOSPHORUS
                                              LOADING  AND  MEAN CHLOROPHYLL a CONCENTRATION
                                              RELATIONSHIP
to
-P
-
Trophic
Water Body State3
Blackhawk (l)d
Brownie ( 2 )
Calhoun (3)
Came lot -Sherwood
Complex (4)
Canadarago
1968 (5-A)
1969 (5-B)
Cayuga
1972 (6-A)
1973 (6-B)
Cedar (7)
Cox Hollow (8)
Dogfish
1972 (10)
Dutch Hollow (11)
George (12)
Harriet (13)
E
E
E

E

E
E

M
M
E
E

0
E
0-M
E
Phosphorus
Loading, L(P)
(mg/m /yr)
2.1 30-2320
1180
860

2350-2680

800
800

800
800
350
1620-2080

20
950-1010
70
710
Mean
Depth ,z
(m)
4 .9
6.8
10.6

3

7. 7
7.7

54
54
6.1
3.8

4
3
18
8.8
Hydraulic
Loading ,q
(m/yr)c
9
3
2

21 .4

12
12

6
6
1
5 .4

1
1
2
3
.8
. 4
.9

-33.3

. 8
.8

.3
.3
.8
-7.6

.1
.7
.2
.7
L(P)A, _
i + /z/qs
1 3 3
144
101

69

35 .1
35.1

32.4
32.4
69.0
160

6.3
246
8.3
75
Mean [loan
Secchi Chlorophyll a
Depth Concentration
(m) (ug/l)b
3
1
2

2


1

2
2
1
1

2
0
8
2
.6
.5
.1

.0

-
.8

.3
.3
.8
.5

.5
.8
.5
.4
15e
6f
6f

63

13
7

6
5
20f
26e

4 (2)g
34e
_
4f

-------
                               Table  23  (continued).   US  OECD DATA APPLIED TO VOLLIItlWEIDER'S
                                         PHOSPHORUS  LOADING AND MEAN CHLOROPHYLL a CONCENTRATION
                                         RELATIONSHIP
CD
cn
Trophic
Water Body State3
Isles (14)
Kerr Reservoir
Roanoke Arm (16)
Nutbush Arm (17)
Lamb
1972 (19)
Meander
1972 (21)
Mendota (22)
Michigan (Open
Waters) (23-A)
Lower Lake23~B)
Minnetonka
1969 (25)
1973 (26)
Potomac Estuary
Upper (28)
Middle (29)
Lower (30)
Redstone (31)
E
E-M
-
-

0

0
E

0
0

E
E->M
U-E
-
-
-
E
Phosphorus Mean
Loading, L(P) Depth, z
r\ \.^
(mg/m /yr) (m)
2030

5200
700

30

30
1200

140
140

500
100(180)k

85000
8000
1200
]440-1680
2.7

10 .3
8.2

4

5
12

84
84

8.3
8.3

4 .8
5 .1
7.2
4.3
Hydraulic
Loading, qg
(m/yr)c
4 .5

51.5
1.6

1.7

1.8
2 .7

2. 8
0 .84

1.3
1.3

120
28. 3
8.5
4.3-6.1
L(P)/qs
1 * ^
254

69.8
134

7.0

6.3
142

7 . 7
15.2

109
21.9(39

590
198
73.4
156
Mean
Secchi
Depth
(m)
1.0

1.1
1.2

2.2

3.0
3.0

	
-

1.5
. "t )kl . 8

0.6
0.9
1.6
1.6
Mean
Chlorophyll a
Concent rat ion
(ug/D
53f

13
21

3 (3)8

2 (l)g
10 (20)h

2
2

21
12

30-150
30-100
10-20
13e

-------
Table 23 (continued).  US OECD DATA APPLIED TO VOLLEHWEIDER'S
         PHOSPHORUS LOADING AND MEAN CHLOROPHYLL a CONCENTRATION
         RELATIONSHIP

Water Body
Sallie
Sammamish
Shagawa
Stewart
Tahoe
East Twin
"3 1973
CO
1974
West Twin
1972
1973
1974

(32)
(33)
(34)
(35)
(36)
(39)
(40)
(41).
(43)
(44)
(45)
Twin Valley(46)
Virginia
Waldo
(47)
(48)
Trophic
State3
E
M
E
E
U-0
E
E
E
E
E
E
E
E
U-0
Phosphorus Mean
Loading, L(P) Depth, z
(mg/m /yr) (m)
1500-4200
700
700
4820-8050
50
700(700)k
500 (500)
700 (500)
400 (400)k
300 (200)
300 (300)
1740-2050
1150-1480
17
6.4
18
5 .7
1.9
313
5
5
5
4 .3
4.3
4. 3
3.8
1.7
36
Hydraulic
Loading, q
(m/yr)c
3.6-5.8
10
7.
23.
0 .
6 .
5 .
10
2 .
2 .
4 .
7.6-9
0.6-1
1.

1
8
45
2
6

7
4
3
.5
.9
7
L(P)
1 + ,
Mean
Secchi
/qs Depth
Jz/q (m)
(lean
Chlorophyll a
Concentration
(Pg/l)b
275
29.
52 .
211
4 .
59 .
45 .
41 .
65 .
53.
34 .
133
44 .
1.
9
0

0
6
8
0
4
4
9

6
8
3
2
1
28
1
2
1
2
2
2
1
1
28
.3
. 3
.4
.3
.6
.3
.9
.2
.8
.3
.5
.2
.0
5
15 (24)1
12e
< lg
26
22
28
40
23
28
19e
29e
< 1^

-------
                                     Table  23 (continued).   US OECD DATA APPLIED TO VULLi:ilWEIDER 'S
                                              PHOSPHORUS LOADING AND MEAN CHLOROPHYLL a  CONCENTRATION
                                              RELATIONSHIP
I—'
co
Water Body
Washington
1957
1964
1971
19 7M
Weir
Wirtgra
EXPLANATION

Mean Mean
Phosphorus Mean Hydraulic i(pw Secchi Chlorophyll a
Trophic Loading, L(P) Depth, z Loading, q_ ' qs Depth Concentration
Statea (mg/ra2/yr)b (m) (m/yr)c " 1 + /z7q"s (m) (pg/l)b
(49) E 1200 33 13.8 34. 1 2.2 12
(50) E 2300 33 13.8 65.3 1.2 20
(51) M 430 33 13.8 12.2 3.5 6
(52) M i(70 33 13.8 13.4 3.8 - (4)
(53) M 140 6.3 1.5 30.6 1.9 8
(54) E 900 2.4 6 91.9 1.3

                                                           M = mesotrophic
                                                           0 = oligotrophic
                                                           U = ultra
                   Based on investigator's estimates.
                  "Hydraulic loading, q  = z/t  = hydraulic residence time  = water  body
                    volume (m )/annual inflow volume (m /yr).

                   ( ) = Identification number for Figures 22, 23 and 24  (see Table 14)

-------
                             Table 23 (continued).   US OECD DATA APPLICD TO VOLLFJ1WEIDER' S
                                      PHOSPHORUS LOADING AND MEAN CHLOROPHYLL a CONCENTRATION
                                      RELATIONSHIP
        EXPLANATION (continued)
        e
         First two meters of water column.
         Summer surface values.
        ^Euphotic zone.
        v.
         Growing season.

        "'"Ice-free period.
        -'Average value for August.
        k
         All data in parentheses represent data received by these reviewers from the principal investigators
         after the completion of this report.  Figure 22 is based on the original data reported by the
         investigators and does not reflect these revised values.  Examination of the revised data indicated
         no significant changes in the overall conclusions concerning these water bodies.
(—'
10        Dash (-) indicates data not available.

-------
IUU
~
3
z
o
(£
1-
Z
LU
2 10
o
o
o|
1
^J
_J

X
Q.
O
tr
o
_i
u '
\j
H
LU
5
i
_*
E
UJ
z
<
1 1 1
LU
2
Oi
.1

: ' ' 28*
29 «/
* 14 /
43 /^
>^jl
"•• ^' fi- •"'
•" "^&>T>C.
... 34 30/ _.
26 |5A*J|; %31B35
r *s^x *22"

- 5I /A5"B 4 , „
• '06-A • 43 ^2
10 /S-B*33

19 X^^
0 /
^r
X" A
w 23~B
23-A
^r
/ • ANNUAL MEAN CHLOROPHYLL 0
A GROWING SEASON CHLOROPHYLL o
Xm CHLOROPHYLL o IN FIRST TWO METER
OF WATER COLUMM
•/ V SUMMER MEAN CHLOROPHYLL i
+ SUMMER SURFACE MEAN CHLOROPHYLL o_
36
48 A
/







—



















* LOG [CHLOROPHYLL g] =076 LOG [(L(p)/qs)/d+./2/qs)] -0259
P-LOADING CHARACTERISTICS
WITHIN EXCEED
j TO1 FRANCF --_!_ i 	 . . Tf\l FDAMfT --
^ i WLcnMix^c. -4- • ----- - --T1 • • ' 1 L/LtKANUb "' •
i i i t i i t i 1 i i i t i i i i 1 i i ill

10 100



S i i

100
                        (mg /m )
Figure 22.
US OECD Data Applied to Vol1enweider
Phosphorus Loading Characteristics and
Mean Chlorophyll a^ Relationship
                               199

-------
     Examination of Figure 22 indicates the investigators have
used a variety of approaches for estimating the chlorophyll a
content of their water.  Some reported values are summer means
while other values are annual means,   Some values are means for
the euphotic zone while others are means for the first two meters
of the water column.  Therefore, in a strict sense the reported
chlorophyll a data for the US OECD water bodies are not dire.ctly
comparable. "However, even with tnese limitations, there is rea-
sonable agreement (r = 0.77) between the predicted trophic states
of the US OECD water bodies, based on their position to the right
or left of the 10 mg/m^ permissible phosphorus concentration boun-
dary line and the investigator's subjective trophic state charac-
terizations.  In general, the results of Figure 22 confirm the re-
sults indicated in the Vollenweider phosphorus loading diagram
(Figure 19).

     Figure 22 also supports some of the possible phosphorus load-
ing estimate discrepancies indicated in Figures 14 and 15.  For
example, based on its phosphorus loading characteristics and mean
chlorophyll a_ concentrations, Lake Weir plots in the eutrophic zone
in Figure 22, in disagreement with the mesotrophic condition indi-
cated by Brezonik and Messer (1975).   This supports the possibility
that the phosphorus loading underestimation indicated in Figures 14
and 15 are in error.  If, on the other hand, the phosphorus loading
estimates for Lake Weir are correct,  then the level of chlorophyll
production per 'unit of input phosphorus must be higher in Lake Weir
than in other water bodies.  This would support the idea of a differ-
ent phosphorus loading-algal response relationship in warm water
bodies compared to that found in water bodies in the north temperate
zones of the US.  Furthermore, the relative closeness of Lake
Dogfish (10), Lake Lamb (19) and Lake Meander (31) to the 10 mg/m3
concentration mark in Figure 22 supports the possible phosphorus
loading underestimations indicated earlier in Figure 15 for these
water bodies.  As indicated earlier,  their reported phosphorus
loadings place them in the trophic zone of the Vollenweider phos-
phorus loading diagram (Figure 19) characteristic of ultra-
oligotrophic Lakes Tahoe and Waldo.  However, Lakes Dogfish, Lamb
and Meander are clearly more productive, in terms of relative
chlorophyll a concentrations, than Lakes Tahoe and Waldo, support-
ing the phosphorus loading underestimation indicated in Figure 15
for these three water bodies.

     In spite of the non-uniform computations of the mean
chlorophyll a concentrations used in Figure 22, the results of
this relationship between phosphorus  loading characteristics,
(i.e., predicted in-lake phosphorus concentration - see Equation
20) and chlorophyll a concentrations indirectly support the
                                 200

-------
validity of the Vollenweider phosphorus loading diagram criteria
illustrated in Figure 19.


US OECD PHOSPHORUS DATA APPLIED IN PHOSPHORUS LOADING AND
SECCHI DEPTH RELATIONSHIP

     The use of the Secchi depth as an indicator of algal bio-
mass has recently been proposed by several investigators
(Edmondson, 1972; Carlson, 1974; Shapiro, 1975b; Shapiro et al.,
1975).  The use of this parameter as an indicator of a water
body's trophic condition is based largely on the public's per-
ception of eutrophication problems.  Remedial treatment programs,
including sewage diversion and advanced waste treatment, have
often been initiated because of the public's reaction to the
side effects of eutrophication, such as dense algal blooms or
decaying algal mats.  As a result, water transparency or clarity
has probably become the most frequently cited all-around general
indicator of water quality.  The higher the transparency of the
water body, the higher is thought to be the general water quality.
Obvious exceptions to this general rule would be water bodies
with high color content.

     Edmondson (1972) has found a close relationship between Secchi
depth and algal biomass (expressed as chlorophyll concentration)
in Lake Washington.  While there are likely some effects due to
light scattering by non-planktonic particles in the water, there is
a definite negative hyperbolic relationship between Secchi depth
and chlorophyll concentration, with the  slope of the curve
steepest at the lower biomass  levels.  This indicates changes in
biomass, as reflected in  chlorophyll concentrations, are more
easily detected in  clear  (i.e., oligotrophic) waters than in
eutrophic waters.   Above  approximately 20 yg/1  chlorophyll con-
centrations, at least in  Lake  Washington, a large increase in
mean  chlorophyll does not  produce  a proportionately large
decrease in Secchi  depth.  This indicates that, above a certain
degree of eutrophication,  Secchi depth readings lose sensitivity
as an indicator of  changes in  algal biomass, other than a low
Secchi depth indicating a  relatively eutrophic  condition of the
water body.

      Even with this  limitation, however, the use of Secchi depth
measurements as an  indicator of a  water  body's  algal biomass,
and hence general trophic  condition, remains an easily measured
parameter, involving a minimum of  time and cost.  In addition,
its meaning is easily understood by the  general public and is a
parameter which can  be  evaluated over time in correlation with
the general trophic  condition  of the water body.

      As the algal biomass  of a water body is related to its
nutrient flux, the  Secchi  depths  of the  US OECD water bodies were
examined as a  function  of  their phosphorus loading characteristics
                                 201

-------
in a manner analagous to that of chlorophyll a  concentration
in Figure 22.   In order to give the plot the same general slope
as expressed in Vollenweider's chlorophyll concentration versus
phosphorus loading characteristics, the reciprocal of the Secchi
depth was plotted versus the phosphorus loading expression,
(L(P)/qs)/(l+\/z;/qs) •  The pertinent data was presented in Table
23.  The US OECD eutrophication study data are presented in
Figure 23.

     Examination of Figure 23 shows a definite relationship does
exist between Secchi depth and phosphorus loadings, with the
reciprocal of the Secchi depth increasing as a function of the
phosphorus loading.  However, the slope is not as steep as that
indicated in Figure 22 between chlorophyll a_ concentration and
phosphorus loading characteristics.  Particularly scattered are
the data sets for the oligotrophic and mesotrophic water bodies.

     In an attempt to graphically produce a greater spread of
data, a semilog plot of the US OECD data was prepared.  This is
illustrated in Figure 24.  Examination of Figure 24 again shows
a relationship exists between these two parameters.  As the
phosphorus loading increases, the reciprocal of the Secchi depth
also increases, with the steepest slope at the higher phosphorus
loading and lower Secchi depth values.  However, the data sets
still exhibit considerable scatter.  Unfortunately, there is not
a sufficient number of oligotrophic water bodies in the US OECD
eutrophication study to allow examination of this relationship,
using US OECD data, other than on a general qualitative basis.
As a nonlinear relationship exists between Secchi depth and
chlorophyll (Edmondson, 1972), it is not surprising to see a
nonlinear relationship existing between phosphorus loading and
Secchi depth, particularly since the algal biomass in a water
body is generally a function of the intensity of the nutrient
flux.  The use of this relationship as a tool for assessing the
expected change in water quality resulting from a changed
phosphorus load will be discussed in a later section of this
report.


US OECD PHOSPHORUS DATA APPLIED IN DILLON'S PHOSPHORUS
LOADING-PHOSPHORUS RETENTION AND MEAN DEPTH RELATIONSHIP

     A different type of phosphorus loading diagram was  subsequently
developed by Dillon  (1975; Vollenweider and Dillon, 1974).  This
loading diagram considers not only the phosphorus loading to a
water body, but also the capacity of the water body to retain
the input phosphorus.  Vollenweider's earlier relationships do
this implicitly as a function of mean depth, z, or hydraulic
loading, qs.  Derivation of Dillon's model was presented in an
earlier section of this report.  Dillon's relationship allows
one to consider the  effects of flushing time, phosphorus loading
and phosphorus retention on the degree of fertility of a water
                                202

-------
               INVESTIGATOR-INDICATED
                  TROPHIC STATE:

                 • -EUTROPHIC
                 A-MESOTROPHIC
                 O-OLIGOTROPHIC
KD
O
CO
CL
LJ
Q


I
O
O

en
o i

                                      2I

                                               52

                                                      33
                                                                     29*
                                                                      • .14
                                                            50     17         47
                                                                   «22
                                                                   12
                            o48    o36
  001
    0.
                       1          1  1  1  1 1
                                                            1    1  1  1  1 1  1 1 1
   Figure  23
                                           10
                                                                          100
1000
                                                 (mg/m3)
                           US  OECD  Data Applied  to  Phosphorus  Loading  and Secchi  Depth
                           Relationship (Log-Log  Scale).

-------
  1.4
  1.2
  1.0
  0.8
Q.
UJ
O
a:
O
O
  0.4
  0.2
             INVESTIGATOR-INDICATED
                TROPHIC STATE'.

               • -EUTROPHIC

               A- MESOTROPHIC

               O- OLIGOTROPHIC
                              19
                              O
                            I0c
                            2U
                                                       28
                                                  .29
                                           50    ..
                                           •   •
                                                      47-
                                         39

                                    26   5
                                     • •
                                     A 53
                                          \30  »31
                                             • 13
                                      45   OIO

                                        44*
                                                .22
                                 51    A33

                                 ^;
                                  '52
                                               '12
                    48
                           36
             I I I Mil
                  I              10            100

                     (L(P)/qs)/(l+v/I7q;)


                           (mg/m3)
                                                          1000
    Figure 24.
                  US  OECD  Data Applied  to Phosphorus
                  Loading  and Secchi  Depth  Relation-
                  ship (Semi log  Scale).
                              204

-------
body.  A main feature of Dillon's model is that since a water
body's phosphorus retention capacity is a function of its flush-
ing rate, consideration of the phosphorus retention coefficient
allows for a more accurate determination of the effects of an ex-
tremely fast or slow hydraulic flushing rate on the phosphorus
loading-trophic response relationship.

     Dillon's phosphorus loading diagram is presented in Figure
25,  The pertinent US OECD data are presented in Table 24.  If
a data range was reported for a water body, the mean value was
used in all calculations.  Phosphorus concentration boundary con-
ditions of 10 yg/1 and 20 yg/1 (Sawyer, 1947; Sakamoto, 1966;
Dillon, 1975) correspond to Vollenweider's permissible and ex-
cessive loading lines, respectively (Figure 19).  The trophic
state associations are similar to those in Figure 19.

     As was found with Vollenweider's phosphorus loading diagram
(Figure 19), water bodies of similar trophic character plot in the
same relative area in Dillon's loading diagram (Figure 25).  There
is generally good agreement between the predicted trophic states
in Dillon's loading diagram and the US OECD investigator-indicated
trophic states.  In addition, Figure 25 supports the possibility
of a phosphorus loading underestimation for Lakes Dogfish (10),
Lamb (19), and Meander (21), indicated in earlier diagrams.


     In general, Dillon's phosphorus loading diagram appears to
be a valid procedure for establishing the relative trophic con-
ditions and phosphorus concentrations of water bodies .  It also
indirectly supports the validity of the Vollenweider phosphorus
loading relationship expressed in Figure 19.  It should be men-
tioned, however, that while Dillon's phosphorus loading diagram
is a substantial improvement over Vollenweider's original phos-
phorus loading and mean depth diagram (Figure 5), it does not
appear to offer any significant improvement over the information
obtained with Vollenweider's modified phosphorus loading and
mean depth/hydraulic residence time loading diagram (Figure 19).
Rather, it is an alternate method for predicting the relative
degree of fertility of a water body.   In fact, Dillon (Vollenweider
and Dillon, 1974) offers his model as a simple method for predict-
ing phosphorus concentrations rather than as a substitute for
Vollenweider's modified phosphorus loading diagram.  It should
be mentioned that Vollenweider's relationship used in Figure 19
(i.e., Equation 9) assumes that R(P)  is expressed solely through
the hydraulic residence time, i^.  However, Vollenweider's rela-
tionship likely would not indicate if any other parameters affected
R(P).  In this regard, Dillon's relationship may be more complete.
                                 205

-------
 E
 X,
 O>
tr
          EUTROPHIC
                 INVESTIGATOR -INDICATED
                    TROPHIC STATE:
                                     • -EUTROPHIC

                                     A-MESOTROPHIC

                                     O-OLIGOTROPHIC
                                       OLIGOTROPHIC
    0.01
                      10             100

                       MEAN DEPTH(m)
                                   1000
    Fi gure  25.
US OECD  Data Applied to Dillon
Phosphorus  Loading  - Phosphorus
Retention  and Mean  Depth
Relationshi p
                            206

-------
                               Table 24.   US OECD DATA APPLIED TO DILLON'S  PHOSPHORUS  LOADING-
                                          PHOSPHORUS RETENTION AND MEAN DEPTH  RELATIONSHIP
r-o
o
Trophic
Water Body State3
Blackhawk (l)e
Brownie (2)
Calhoun (3)
Camelot-Sherwood ( 4 )
Canadarago
Cayuga (6)
Cedar (7)
Cox Hollow (8)
Dogfish (10)
Dutch Hollow (11)
George (12)
Harriet (13)
Isles (14)
Kerr Reservoir
Roanoke (16)
Nutbush (17)
Lamb (19)
Meander (21)
Mendota (22)
E
E
E
E
E
M
E
E
0
E
0-M
E
E
E-M
0
0
E
Phosphorus
Retention ,
Coefficient, R
0 .
0.
0.
0.23
0.
0.
0.
0.41
0.
0.
0.
0.
0 .
0.
0.
0.
0.
0.
41
59
66
-0.27
44
75
64
-0.46
65
57
74
61
44
31
69
60
62
68
Phosphorus
Loading, L
(g/m /yr)
2 .
1
0
1-2.3
.18
. 86
2. 35-2.68
0
0
0
. 8
.8
. 35
1.62-2 .08
0
.02
0.95-1.01
0
0
2
5
0
0
0
1
.07
.71
.03
.2
. 7
. 03
.03
. 2
Flushing
Rate, p
(yr-V
2
0
0
7.
1
0
0
1.
0
0
0
0
1
5
0
0
0
0
.0
. 5
.28
1-11.1
.67
.12
.30
4-2. 0
.29
. 56
.12
.42
.67
.0
.20
.44
.37
.22
L(l-R)/p
2
(mg/m )
0
0
1
0
0

0
0
0
0
0
0
0
0
1
0
0
1
.70
.98
.05
.21
.27

.41
.61
.02
.75
.15
.66
.69
.72
.08
.03
.03
.73
Mean Depth, z
(m)
4
6
10
3
7
54
6
3
4
3
18
8
2
10
8
4
5
12
.9
.8
.6
. 0
.7

.1
.8
.0
.0

.8
. 7
. 3
.2
.0
.0


-------
Table 24 (continued).   US  OKCD DATA APPLIED TO DILLON'S PHOSPHORUS
         LOADING-PHOSPHORUS RETENTION AND MEAN DEPTH RELATIONSHIP
Water Body
Trophic
State3
Phosphorus
Retention ,
Coefficient, R
Phosphorus
Loading, L
(g/m2/yr)C
Flushing
Rate , p
L(1-R)/P Mean Depth, ?.
(mg/m ) (m)
Michigan (Open Waters)
1971 (23-A)
1974 (24-A)
1971 (23-B)
1974 (24-B)
0
0
0
0
0.
0.
0.
0.
84
84
91
91
0.
0 .
0 .
0.
14
1
14
10
0 .
0 .
0 .
0.
03
03
01
01
0
0
1
0
.72
.51
.27
.91
84
84






Lower Lake Minnetonka c
1969 (25)
1973 (26)
^J Potomac Estuary
<=> Upper (28)
Middle (29)
Lower (30)
Redstone (31)
Sallie (32)
Sammamish (33)
Shagawa (34)
Stewart (35)
Tahoe (36)
East Twin
]972 (39)
1973 (40)
1974 (41)
E
E^M
U-E
-
-
-
E
E
M
E
E
U-0

E
E
E
0.
0.

0.
0.
0.
o . M r,
0. 51
0.
0.
0.
0 .

0.
0.
0.
72f
72f

17
3
48
-0.50
-0.57
57
47
22


47
49
41
0.
0.

85
8
I.
1.44-
1.5-
0.
n .
4.82-
0.

0.
0.
0.
5
1 (0.2)1



2
1. 68
4.2
7
7
8 .05
OS

7 (0.7V
5 (0.5)
7 (0.8)
0.
? 0.

25
5.
1.
1. 0-
16
16


56
18
1.4
0. 56-0.91
0.
1.
12 .
0.

5 1.
1 .
? .
56
25
5
001

25
11
0
0
0

2
1
0
0
1
0
0
0
1

0
0
0
.88
.18 (0.35)g

.83
.01
.53
.68
.78
.54
. 30
. 40
.53

30 (0.30)g
.23 (0.23)
21 (0.24)
8 .
8 .

4 .
5.
7 .
4.
6.
18
5.
1.
313

5.
5.
5.
3
3

8
1
2
3
4

7
9


0
0
0

-------
   Table  24  (continued).  US OP.CD  DATA APPLIED TO DII,LOU'S  PHOSPHORUS
            LOADING-PHOSPHOPUS RrTrHTICHI AtlD MEAN DEPTH  RELATIONS!! IP
Trophic
Water Body State3
West Twin
1972 (43)
1973 (44)
1974 (45)
Twin Valley (46)
Virginia (47)
Waldo (48)
Washington
1957 (49)
1964 (50)
1971 (51)
1974 (52)
Weir (53)
Wingra (54)
EXPLANATION:
a

E
E
E
E
E
U-0

E
E
M
M
M
E


Phosphorus
Retention .
Coefficient, R

0.
0.
0.
0. 39
0.49
0.

0.
0.
0.
0.
0.
0.



56
57
50
-0.41
-0.63
82

61
61
61
61
67
39


Phosphorus Flushing
Loading, L Rate, p
(g/m /yr) (yr )

0.
0.
0.
1.74-2
1.15-1
0.

1 .
2.
0.
0.
0.
0.



4 (0.
3(0.
3(0.
.05
.48
017

2
3
43
47
14
9



4)g o
2) 0
3) i
2.0-
0. 36-
0

0
0
0
0
0
2



.62
.56
.0
2 . 5
1. 1
.05

.42
.42
.42
.42
. 24
. 5


L(1-R)2/P Mean Depth, ?,
(mg/m ) (m)

0
0
0
0
0
0

1
2
0
0
0
0



28 (0.28)g
;23<°.K>
.15(0.15)
.51
.80
.06

.11
.14
.40
.44
.20
. 22



4
4
4
3
1
36

33
33
33
33
6
2



. 3
. 3
.3
.8
. 7






. 3
.4


E = eutrophic
M = raesotrophic
0 = oligotrophic
U = ultra

-------
                      Table 24 (continued).  US OECD DATA APPLIED TO DILLON'S PHOSPHORUS
                               LOADING-PHOSPHORUS RETENTION AND MEAN DEPTH RELATIONSHIP
EXPLANATION (continued)
     Retention coefficient, R= 1 / ( 1 + Jp((J ) ,  where p  = 1/T  = I/hydraulic residence time ( Vollenweider ,
          1975a; 197Ba).  See Table 20 for hydrauTic residence time for US OECD water bodies.
Flushing rate, p = (discharge (m /yr) /water body volume (m )  = I/T ).
     Based on investigator's estimates.

     Flushing rate, p = (discharge (m /
    p
     Identification number for Figure 25 (See Table 14).
     Whole lake value .
    £
     Data in parentheses represents  data received  by these  reviewers from the principal investigators
     subsequent to completion of  this report.   Figure 25 is based  on the original data reported by
     the  investigators  and does not  reflect  the revised  data.   Examination of the revised data
     indicated  no significant changes in the overall conclusions concerning these water bodies.

-------
US OECD PHOSPHORUS DATA APPLIED IN LARSEN AND MERCIER'S INFLUENT
PHOSPHORUS AND PHOSPHORUS RETENTION RELATIONSHIP

     Larsen and Mercier (1976) proposed another alternate method
of examining the nutrient loading-trophic response relationships
in water bodies.  Consistent with the view that the phosphorus
concentration in a water body, rather than the phosphorus loading
to the water body, ultimately controls algal blooms and the
eutrophication process (Vollenweider, 1968; Vollenweider and Dillon,
197M-), Larsen and Mercier (1976) devised a phosphorus loading dia-
gram which related a water body's trophic state to its influent
phosphorus concentration, as modified by its phosphorus retention
coefficient, R(P).  They described the mean phosphorus concentration
in a water body as the relationship between its mean influent
phosphorus concentration and^ its ability to assimilate this influent
phosphorus .  The derivation of this approach was presented in an
earlier section of this report.  The Larsen-Mercier approach of
utilizing the water body influent phosphorus concentrations rather
than the phosphorus loading may be particularly important for water
bodies that receive a substantial part of their key limiting nu-
trient load in a form that is not immediately available for aquatic
plant growth.  An example would be the phosphorus present in ero-
sional material.  In such cases, the phosphorus loading would not
accurately predict the ultimate aquatic plant growth within the
water body.  As indicated earlier, Cowen et al. (1976a) have found
that typically up to 20 percent of the norfsbluble orthophosphate
present in US tributaries to Lake Ontario is available for algal
growth in Lake Ontario.

     Curves delineating trophic zones can be drawn on Larsen and
Mercier's loading diagram, analogous to the trophic zones in the
Vollenweider phosphorus loading diagram (Figure 19).  The relative
degree of eutrophy or oligotrophy of a water body is a function of
its vertical displacement above or below the permissible phosphorus
concentration line.  The permissible and excessive phosphorus con-
centration lines correspond to the 10 yg/1 and 20 yg/1 limits
determined by Sawyer (1947) and Sakamoto (1966), respectively.
They are included in the loading diagram, according to Larsen and
Mercier (1976), mainly for "illustrative purposes."

     The Larsen and Mercier diagram, containing the US OECD water
bodies, is presented in Figure 26.  The pertinent US OECD data
are presented in Table 25.  If a data range was reported for a
water body, the mean value was used in all calculations.  General-
ly, the results of Figure 26 agree with those of Figures 22 and 25.
In most cases, the predicted trophic states are in agreement with
those reported by the US OECD investigators.  A feature of Larsen
and MercierTs relationship is that it allows one to relate the mean
phosphorus concentration of a water body to both its phosphorus
loading and its mean influent phosphorus concentration.  If two
of the above parameters are known, one can use the interrelation-
ship between the three components to determine the value of the
third parameter.


                                211

-------
g

i-

K
H
z
Ul


o —
r>
tr
o
x
Q.


O
X
Q.
UJ




U.
      100
       20
       10
         0
                        ._-,,-  - -!- INDICATED
                        TROPHIC STATED	
                      • -EUTROPHIC

                      A- MESOTROPHIC

                      O- OLIGOTROPHIC
                   J	I	I 	I
                                          1
                                                1
 OLIGOTROPHIC


	I	i	
                   0.2         0.4        0.6        0.8         1.0


                 PHOSPHORUS RETENTION  COEFFICIENT. R
 Figure  26.   US OECD  Data Applied  to Larsen and  Mercier

               Influent Phosphorus and Phosphorus  Retention

               Relationship
                                   212

-------
Table 25.   US OECD DATA APPLIED TO  LARSEN AND MERCIER'S
           INFLUENT PHOSPHORUS  CONCENTRATION AND
           PHOSPHORUS RETENTION RELATIONSHIP
Water Body Trophic State3
Blackhawk (l)d
Brownie (2)
Calhoun (3)
Camelot-Sherwood (4)
Canadarago (5)
Cayuga (6)
Cedar (7)
Cox Hollow (8)
Dogfish (10)
Dutch Hollow (11)
George (12)
Harriet (13)
Isles (14)
Kerr Reservoir
Roanoke (16)
Nutbush (17)
Lamb (19)
Meander (21)
Mendota (22)
Michigan (Open Waters)
(23-A)
(24-A)
(23-B)
(24-B)
Lower Lake Minnetonka
1969 (25)
1973 (26)
Potomac Estuary
Upper (28)
Middle (29)
Lower (30)
Redstone (31)
E
E
E
E
E
M
E
E
0
E
0-M
E
E
E-M
-
-
0
0
E
0
0
0
0
E
E-*M
U-E
-
-
E
Phosphorus
Retention .
Coefficient ,R
0.41
0.59
0 .66
0.25
0 .44
0.75
0 .64
0 .44
0 .65
0 .57
0.74
0 .61
0 .44

0.31
0.69
0 .60
0.62
0 .68
0.84
0 .84
0 .91
0.91
0.728
0.72e
0.17
0 .30
0 .48
0 .48
Influent Phosphorus
Concentration, [p]
(yg/Dc
227
347
297
92 .0
62.4
127
194
285
18.2
576
32
192
451

101
438
17.6
16.7
444
50
36
167
119
417
76.9- (138 )f
708
283
142
300
                            213

-------
Table 25 (continued).   US  OECD  DATA APPLIED  TO  LARSEN AND
         MERCIER'S INFLUENT  PHOSPHORUS  CONCENTRATION AND
         PHOSPHORUS RETENTION RELATIONSHIP

Phosphorus
Retention ,
Water Body Trophic State3 Coefficient ,R
Sallie (32)
Sammamish (33)
Shagawa (34)
Stewart (35)
Tahoe (36)
East Twin
1972 (39)
1973 (40)
1974 (41)
West Twin
1972 (43)
1973 (44)
1974 (45)
Twin Valley (46)
Virginia (47)
Waldo (48)
Washington
1957 (49)
1964 (50)
1971 (51)
1974 (52)
Weir (53)
Wingra (54)
EXPLANATION:
Investigator-indicated
E = eutrophic
M = mesotrophic
0 = oligotrophic
U = ultra
E
M
E
E
U-0

E
E
E

E
E
E
E
E
U-0
E
E
M
M
M
E

trophic state :

0 .54
0 .57
0 .47
0 .22
0 .96

0 .47
0.49
0 .41

0.56
0 .57
0 .50
0 .40
0.56
0 .82
0.616
0.61e
0 .61e
-0 .61e
0 .67
0.39



Influent Phosphorus
Concentration, [p]
(yg/l)c
606
70
98 .6
270
111

113
89 .3
70.0

148
125
69 .8
222
1052
10 .1
87.0
167
31.2
34.0
46.7
150









(113)f
(89.3)
( 8 0 '. 0 )

(148)
(83.8)
(69.8)












                               214

-------
        Table 25 (continued).  US OECD DATA APPLIED TO LARSEN AND
                 MERCIER'S INFLUENT PHOSPHORUS CONCENTRATION AND
                 PHOSPHORUS RETENTION RELATIONSHIP
EXPLANATION (continued).


 Retention coefficient, R = I/ (1+ /JO >  where p^ = I/TW = I/hydraulic
       residence time (Vollenweider,  I975a;  1976a).  See Table 20
       for hydraulic residence times  of  US OECD water bodies.

cMean influent phosphorus concentration,  [P] = L(P)/qs,
       where L(P) = phosphorus loading (mg/m^/yr) and qs =
       hydraulic loading = Z/TU,  where z  = mean depth (m) and
       To) ~ hydraulic residence time.  See Table 15 for influent
       phosphorus concentrations  for  US  OECD water bodies.

 Identification number for Figure 26  (see Table 14).

eWhole lake value.

 All  -data in parentheses  represents data  submitted  to these reviewers
 from the principal  investigators  subsequent to  the  completion of
 this report.   Figure  26  is based  on  the  original data  submitted by
 the  investigators  and does not reflect the revised  data.  Exam-
 ination  of the revised data  indicated no  significant changes  in
 the  overall conclusions  concerning these water  bodies.
                                   215

-------
     In summary, the results of Vollenweider's phosphorus loading
characteristics and mean chlorophyll a concentration relationship
(Figure 22), Dillon's phosphorus loading/phosphorus retention and
mean depth relationship (Figure 25) and Larsen and Mercier's
influent phosphorus concentration and phosphorus retention rela-
tionship (Figure 26), all either directly or indirectly support
Vollenweider's approach for estimating critical phosphorus loads
for lakes and impoundments.  Furthermore, they generally support
the possible errors in the phosphorus loading estimates suggested
in Figures 14 and 15.  This supports both the validity of the
Vollenweider relationship illustrated in Equation 26 , and the
use of watershed land use nutrient export coefficients as methods
of estimating phosphorus loadings and of checking the reasonable-
ness of calculated phosphorus loadings.  Finally, these three
models offer a certain capacity, based on the phosphorus loadings,
for predicting the mean phosphorus and mean chlorophyll a_ concen-
trations in a water body.                               ~~
                                   216

-------
                             SECTION  X

               CORRELATIONS BETWEEN NUTRIENT LOADINGS
               AND EUTROPHICATION RESPONSE PARAMETERS


     This section of this report is devoted to analysis of the cor-
relations between the nutrient loading for the US OECD water bodies
and their eutrophication response to these loadings.  A list of
suggested correlations was developed by R. Vollenweider and mem-
bers of the OECD Eutrophication Technical Bureau and was dis-
tributed to the OECD eutrophication principal investigators.  Many
of these suggested correlations could not be made for the lakes in
the US OECD eutrophication study since only a limited number of
investigators had data for all of the parameters required to make
these correlations.  Included in the list of suggested eutrophica-
tion response parameters were maximum rates of primary production
and respiration, stratified period average chlorophyll a_ content,
average epilimnetic concentration of particulate phosphorus , areal
hypolimnetic oxygen deficit, maximum oxygen surplus , duration of
algal blooms and maximum rate of development of bloom.  These data
were not reported for the US OECD water bodies .  In some instances,
insufficient data were available to prepare a potentially meaning-
ful plot of the data.  For some parameters, the correlations have
been presented and discussed in previous sections of this report .
This section of this report presents what might be considered mis-
cellaneous correlations which are thought to be of lesser importance
than those presented in other parts of the report or where there
were insufficient data to justify a more intensive discussion of
the relationship.  A listing of the various correlations analyzed
in this report is presented in Table 26.

     Before presenting the results of these correlations between
nutrient loadings and eutrophication response parameters, the
reader should be made aware of several factors which limit the
values of these analyses.  First, as indicated in an earlier sec-
tion of this report (Table 11), the various response parameters
(i.e., nutrient concentrations) were measured using a variety of
analytical techniques.  In addition to differing analytical pro-
cedures, the sampling methodologies also varied widely,which could
affect the results obtained for a given response parameter measure-
ment.  As indicated in the Summary Sheets (Appendix II), the US
OECD water bodies were sampled at a variety of depths and locations
and on differing dates.  For example, some water bodies were
sampled frequently all year, others were sampled frequently part
of the year and less frequently the rest of the year, while still


                                   217

-------
       Table  26.  LIST  OF  CORRELATIONS  EXAMINED IN US OECD
                 WATER BODIESa
  I.   Phosphorus  Loading  and:
      A.   annual  mean  chlorophyll a  (Figure 27);
      B.   annual  mean  Secchi depth  (Figure 28);
      C.   annual  mean  total phosphorus  (Figure 29);
      D.   annual  mean  dissolved  phosphorus (Figure  30);
      E.   annual  primary  productivity  (Figure  31);
      F.   annual  total primary production  (Figure 32);
      G.   growing season  epilimnetic chlorophyll a  (Figure  33);
      H.   growing season  epilimnetic total phosphorus   (Figure 34);
      I.   growing season  epilimnetic dissolved phosphorus
                                                       (Figure 35);
      J.   growing season  epilimnetic primary productivity
                                                       (Figure 36) ;
      K.   spring  overturn total  phosphorus (Figure  37);
      L.   spring  overturn dissolved  phosphorus*
 II.   Nitrogen Loading and:
      A.   annual  mean  chlorophyll a  (Figure 38);
      B.   annual  mean  Secchi depth  (Figure 3S);
      C.   annual  mean  inorganic  nitrogen  (Figure 40);
      D.   annual  primary  productivity  (Figure 41);
      E.   annual  total primary production  (Figure 42);
      F.   growing season  epilimnetic chlorophyll a  (Figure  43);
      G.   growing season  epilimnetic inorganic nitrogen  (Figure 44);
      H.   growing season  epilimnetic primary productivity
                                                        (Figure 45);
      I.   spring  overturn inorganic  nitrogen (Figure 46).
III.   Annual Mean Total Phosphorus  and:
      A.   annual  mean  chlorophyll a  (Figure 47);
      B.   annual  mean  Secchi depth  (Figure 43);
                              218

-------
        Table 26 (continued).  LIST OF CORRELATIONS EXAMINED
                IN US OECD WATER BODIES
       C.  annual mean dissolved phosphorus (Figure 49);
       D.  annual primary productivity (Figure 50);
       E.  growing season epilimnetic chlorophyll a (Figure 51);
       F.  growing season epilimnetic primary productivity
                                                      (Figure 52);
       G.  spring overturn total phosphorus (Figure 53).
  IV.  Growing Season Epilimnetic Total Phosphorus and:
       A.  growing season epilimnetic chlorophyll a (Figure 54);
       B.  growing season epilimnetic primary productivity
                                                      (Figure 55).
   V.  Spring Overturn Total Phosphorus and:
       A.  growing season epilimnetic chlorophyll a_ (Figure 56);
       B.  growing season epilimnetic total phosphorus   (Figure  57);
       C.  growing season epilimnetic dissolved phosphorus
                                                      (Figure 58) .
  VI.  Annual Mean Dissolved Phosphorus and:
       A.  annual mean chlorophyll a (Figure  59);
       B.  annual primary productivity (Figure 50);
       C.  spring overturn dissolved phosphorus (Figure  61).
 VII.  Growing Season Epilimnetic Dissolved Phosphorus  and:
       A.  growing season epilimnetic chlorophyll a_ (Figure 62).
VIII.  Spring Overturn Dissolved Phosphorus and:
       A.  growing season epilimnetic chlorophyll a (Figure £3);
       B.  growing season epilimnetic dissolved phosphorus
                                                       (Figure  6^;;
       C.  growing season epilimnetic primary productivity*
                          219

-------
      Table 26 (continued). LIST OF CORRELATIONS EXAMINED
              IN US OECD WATER BODIES
 IX.   Annual Mean Inorganic Nitrogen and:
      A.  annual mean chlorophyll a (Figure 65);
      B.  annual mean Secchi depth (Figure 66);
      C.  annual primary productivity (Figure 67);
      D.  growing season epilimnetic chlorophyll a (Figure 68);
      E.  growing season epilimnetic primary productivity
                                                      (Figure 69);
      F.  spring overturn inorganic nitrogen*
  X.   Growing Season Epilimnetic Inorganic Nitrogen and:
      A.  growing season epilimetic chlorophyll a_ (Figure 70)
      B.  growing season epilimnetic primary productivity
                                                      (Figure 71).
 XI.   Others^:
      A.  annual primary productivity and  annual mean chlorophyll  a
                                                      (Figure 72) ;
      B.  annual mean chlorophyll a and annual mean Secchi depth
                                  ~       (see Figures 77  and 78 )j
      C.  annual primary productivity and  mean Secchi depth
                                                      (Figure 73);
      D.  growing season mean primary productivity and growing
            season mean chlorophyll a  (Figure 7U);
      E.  annual mean daily primary productivity and annual mean
            chlorophyll a (Figure 75);
      F.  annual mean daily primary productivity and annual mean
            areal chlorophyll a (Figure 75).

aData taken from Summary Sheets (Appendix  II).
Insufficient data available.
                             220

-------
others were sampled infrequently all year.  Also, some reported
mean values were arithmetic means of several sampling depths,
others were mean values integrated over the sampling depths, while
still others were surface or epilimnetic mean values.  As discussed
in an earlier section, these factors  can all contribute to an
erroneous "mean" value for a given response parameter measurement.
It is not possible to determine the extent of possible errors in
the parameters used in the correlations.  This section presents
a general idea of the correlation(s)  that may exist between nu-
trient loads and eutrophication response parameters in the US OECD
water bodies.  No statistical evaluation of the correlation data
was undertaken.  This report is limited to a simple visual examina-
tion of the correlations in a graphical form for obvious trends.
A 'correlation' as used in this section of the report indicates
that a relationship, either positive or negative, appears to
exist between two parameters on the basis of a visual inspection
of a plot of these two parameters.  No attempt is made in these
plots to indicate the particular water body responsible for the
data.  All data used in these plots are available in Appendix II.
For some plots, the investigator-indicated trophic status is pre-
sented.  For others, where there are obvious differences in the
types of data for some parameters, this is also indicated on the
plot .

PHOSPHORUS LOADINGS

     Although there is a large amount of scattering of the data,
there is a correlation observed between phosphorus loading and
mean chlorophyll a (Figure 27).  The scatter in this diagram, as
well as all other~correlations examined in this section, is partly
due to sampling and analysis variability, as indicated earlier.
In addition, the  'mean' chlorophyll a consisted of annual means,
summer means and annual mean chlorophyll in the upper two meters
of the water column.  As algal growth is dependent on the loading,
the correlation is expected.  However,  there usually is no clear
correlation between phosphorus loading and the resulting algal
biomass (as indicated by chlorophyll  a content) in a water body
(Vollenweider, 1968; Vollenweider and Dillon, 1974).  It depends
on a number of factors discussed earlier, such as the mean depth
of the water body and its hydraulic residence time.  Consequently,
Figure 22, which incorporates the phosphorus loading to a water
body, as modified by its assimilative capacity (i.e., (L(P)/qs)/
(1+ (TW) , is a much better indicator of the phosphorus loading-
chlorophyll response of a water body.  Vollenweider (1976a) has
shown a good correlation between these two parameters.  The US
OECD water bodies also show a good correlation (Figure 22).

     There is a correlation between phosphorus loading and mean
Secchi depth (Figure 28).  The relationship is a negative hyper-
bolic function on this semi-log plot, although it exhibits a cer-
tain degree of scatter.   A negative relationship between Secchi
depth and chlorophyll a has been reported by Edmondson (1972)
and Carlson (1974).   STnce phosphorus loading is correlated with

                                221

-------
   lOOr-
O>
ol
     10
ro
ro
i-o
°
o:
O
—1
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                PHOSPHORUS LOADING (gP/mz/yr)
        Figure  27.   Phosphorus  Loading and Mean Chlorophyll a
                    Relationship  in US OECD Water Bodies

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                  Figure 28.   Phosphorus Loading and Mean Secchi  Depth
                              Relationship in  US OECD Water Bodies

-------
chlorophyll (Figure 22) and chlorophyll Is correlated with Secchi
depth, then a correlation should, and does, exist between phos-
phorus loading and Secchi depth.  This relationship will be used
in a following section of this report to indicate how changes in
water quality can be related to changes in phosphorus loadings to
a water body.

     A positive correlation exists between phosphorus loading and
mean total phosphorus in the water body (Figure 29).  Although
there is considerable data scatter, this correlation is not un-
expected since the total phosphorus content of a water body will
usually be a function of the input phosphorus.  Contrastingly,
there is not a readily observable correlation between phosphorus
loading and the mean dissolved phosphorus concentrations in the
US OECD water bodies (Figure 30), in view of the considerable
scatter of the data.  This lack of correlation is expected since
dissolved phosphorus is the algal-available phosphorus form and
will be readily assimilated by the algal population in a water
body.  It is expected that, in general, the available nutrients,
both phosphorus and nitrogen, will not show a good correlation
with any of the parameters examined in this section.  The avail-
able nutrient concentration will increase and decrease in a water
body, depending on the algal growth dynamics which fluctuate con-
siderably during the annual cycle.

     There appears to be a positive correlation between areal
phosphorus loading and mean annual primary productivity (Figure 31)
although the data are scattered and limited.  In general, correla-
tions between primary productivity and both nutrient loadings and
concentrations, although usually present, were marked by consider-
able data scatter.  This rendered this eutrophication response
parameter of limited value.  In addition, the question of macro-
phyte and attached algal primary production was not addressed in
this study.  In contrast with primary productivity, there is no
readily observable correlation between phosphorus loading and
total primary production (i.e., g C/yr in the water body) in the
US OECD water bodies (Figure 32).  The total production, as a
function of phosphorus loading, appears to vary widely.

     A positive correlation appears to exist between phosphorus
loadings and the growing season epilimnetic concentrations of
both chlorophyll a and total phosphorus (Figures 33 and 34, re-
spectively).  (Note:  the growing season, as used in this report,
was the period between May and October.  However, the growing
season varied considerably between water bodies, being less for
some water bodies and considerably longer for others such as
the Kerr Reservoir and Lake Weir.  Since such differences in
growing season could not be standardized, all "growing season"
values, regardless of length of growing season, were assumed to
be equivalent in the correlations).   By contrast, there is no
correlation between phosphorus loading and the growing season
dissolved phosphorus concentration (Figure 35).  As indicated
above, this is not unexpected since the dissolved phosphorus con-
centrations will vary as a function of algal growth, rather than

                                 224

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                                  Figure 29.   Phosphorus  Loading and Mean Total  Phosphorus
                                               Relationship in US OECD Mater Bodies

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              Figure 31.   Phosphorus Loading and Primary Productivity

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                                          Chlorophyll  a Relationship  In  US OECD Water Bodies

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                                 Figure 35.  Phosphorus  Loading and Growing Season EpHimnetic

                                             Dissolved  Phosphorus Relationship in US OECD
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-------
phosphorus supply.  It is not clear whether there is a correlation
between phosphorus loading and growing season primary productivity
(Figure 36) mainly because of scarcity of data.  The growing sea-
son primary productivity was not measured in most US OECD water
bodies.  There appears to be a poor correlation between phosphorus
loading and the spring overturn total phosphorus (Figure 37), al-
though there is also a scarcity of data for this correlation.
This is somewhat surprising since the total phosphorus throughout
the year should generally be a function of the input phosphorus.
There are not sufficient data available to examine the correlation
between phosphorus loading and spring overturn dissolved phos-
phorus.  A reasonably good correlation should be found for these
two parameters for lakes which normally have ice cover during the
winter.

NITROGEN LOADINGS

     It should be noted before examining the correlations between
nitrogen loadings and eutrophication response parameters that
most of the US OECD water bodies are phosphorus-limited (Table 9)
with respect to algal growth requirements.   Nitrogen loadings
were not reported for a number of the US OECD water bodies with
the result that the US OECD data base for nitrogen loads is less
extensive than that for phosphorus loads .  The application of any
of the correlations in this section for providing justification
for a certain type of eutrophication control measure should be
made with caution.

     A positive correlation was found between nitrogen loading and
mean chlorophyll a (Figure 38).  The correlation _is very similar
to that seen between phosphorus loading and chlorophyll a
(Figure 26).  There is an order of magnitude increase on~the load-
ing axis of the graph, but the relative positions of the water
bodies are similar.  This illustrates the relatively constant in-
put of nitrogen relative to phos-Dho^us.  This is consistent with
Vollenweider's (1968) use of a ]5N:1P loading ratio (by weight'
in his original loading diagrams (Figures 5 and 6).  Since most
of the US OECD water bodies are phosphorus-limited, one must view
the positive correlation between nitrogen loading and mean chloro-
phyll a with caution.  The relatively constant N:P loading ratio
may be~producing an artifact with respect to this relationship.
This possibility is illustrated in examination of the correlation
between nitrogen loadings and Secchi depth  (Figure 39).  Although
there are fewer data points than with phosphorus loads, there is  a
considerable amount of data scatter in this relationship (i.e., a
nitrogen load of approximately 2 g N/m^/yr  producing a Secchi
depth range of about 2 to 9 meters, to cite one example).  This
would suggest that the nitrogen load has less effect on the algal
populations, and hence resultant Secchi depth, than does the
phosphorus load.  This view is consistent with a phosphorus-limita-
tion of most US OECD water bodies.

     A high positive correlation is found between nitrogen load-
ing and mean inorganic nitrogen (Figure 40).  The correlation

                                  232

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                          Figure 37.  Phosphorus Loading and  Spring Overturn Total
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-------
Is better than that found between phosphorus load and either mean
total phosphorus or mean dissolved phosphorus (Figure 29 and 30).
This strong positive nitrogen loading-mean inorganic nitrogen cor-
relation lends support to the view that most US OECD water bodies
are phosphorus-limited, rather than nitrogen-limited.  This high
correlation Indicates that the algal populations are not in general
depleting the input nitrogen, regardless of the magnitude of the
input.   Rather, the inorganic nitrogen (i.e., algal-available
nitrogen) is increasing as the loading is Increasing.  Thus, the
algae are not growing in response to the input nitrogen, but
rather in response to another nutrient.  The lack of correlation
between phosphorus loading and mean dissolved phosphorus (Figure 30)
indicates the controlling nutrient is likely phosphorus.

     There appears to be a positive correlation between nitrogen
loading and primary productivity (Figure 41).  The correlation ap-
pears to be about the same degree as that between phosphorus load-
ing and primary productivity (Figure 31).  However, there are
fewer data sets for nitrogen loading than for phosphorus loading.
Thus, this nitrogen load-primary productivity correlation may also
be a coincidental artifact of the relatively constant N:P loading
ratio found with the US OECD water bodies.  There appears to be
no readily observable correlation between total annual primary
production and nitrogen loading (Figure 41).  The data scatter is
of the same magnitude 3.5 that between phosphorus loading and total
annual primary production (Figure 32).  This further illustrates
the limited applicability of correlations between nutrient loads
and both primary productivity and total production.  It indicates
the relationship between these parameters may be more complex than
can be visualized using this single graphing technique.

     It is difficult to determine if there is a correlation between
nitrogen loading and growing season epilimnetic chlorophyll a
(Figure 43).  The correlation may be real, but the scarcity of
data for these two parameters does_not allow an accurate evalua-
tion.  For the common water bodies, the data scatter between these
two parameters appears to be as great as that seen between phos-
phorus loading arid growing season epilimnetic chlorophyll a_
(Fi^uie 33).  There Is a better' correlation between nitrogen
loading and growing season epilimnetic inorganic nitrogen (Figure
4'i ) than between phosphorus loading and either growing season
epilimnetic total phosphorus or dissolved phosphorus (Figures 29
^ini ^ 0 , respectively).  Thus, the growing season and annual
mean -i Igal-ava i L-ible nitrogen both seem to correlate reasonably
well with their input.  This growing season correlation (i.e.,
deDendence) of inorganic nitrogen upon the nitrogen loading pro-
vides fin?fher support to phosphorus-limitation of most of the US
OL D water bodies.

     While a positive correlation is seen between nitrogen loading
and growing season epilimnetic primary productivity (Figure 45),
the data are too scarce to draw any clear conclusions as to the
validity oT this relationship.  It is likely this correlation is

                                238

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                             Figure  41.  Nitrogen  Loading and Primary  Productivity
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a coincidental artifact of phosphorus-limitation.   Finally, there
appears to be no correlation between nitrogen loads and the spring
overturn concentration of inorganic nitrogen (Figure 4-6).  It
should be noted, however, that as with phosphorus  (Figure 37), a
majority of the data sets include the mean winter  concentration
rather than the spring overturn concentration.   How this difference
may affect the results obtained with these correlations is not
known.

MEAN TOTAL AND DISSOLVED PHOSPHORUS CONCENTRATIONS

     A positive correlation was observed between total phosphorus
and mean chlorophyll a in the US OECD water bodies (Figure 47)
even though the  'mean' values consisted of annual  means, ice-free
period means and summer means.  Dillon and Rigler  (1975) and Jones
and Bachmann (1976) have also reported high correlations between
these two parameters.  A negative correlation was  also seen
between mean total phosphorus and mean Secchi depth (Figure 48).
This is to be expected since Secchi depth is a negative hyperbolic
function of the chlorophyll content of a water body (Edmondson,
1972; Carlson, 1974; Dillon and Rigler, 1975).   Since chlorophyll
is correlated with mean total phosphorus, mean Secchi depth should
also be correlated with mean total phosphorus,  as  was observed.
A high positive correlation was noted for the mean total phos-
phorus and the mean dissolved phosphorus (Figure 49).  This is not
surprising since the dissolved phosphorus content  of the water body
should be related to the total phosphorus content.  This correla-
tion indicates the dissolved phosphorus appears to be a relatively
constant fraction on an annual basis of the total  phosphorus in
the US OECD water bodies.  The mean total phosphorus also appears
to be positively correlated with- the mean primary  productivity
(Figure 50).  The correlation between these two parameters is
better than that seen between the phosphorus or nitrogen loading
and mean primary productivity (Figures 31 and 32,  respectively).

     In general, although positive correlations are noted, the
data are too scarce to make any valid conclusions about the rela-
tionship between mean total phosphorus and either  the growing
season epilimnetic chlorophyll a or primary productivity (Figures
51 and 52, respectively).  A positive correlation  may exist
between mean total phosphorus and the spring overturn total
phosphorus (Figure 53), although the data are also  relatively
scarce for this relationship.  It should also be noted that a
majority of the water bodies in Figure 53 have  mean winter total
phosphorus concentrations plotted rather than the  spring overturn
concentrations.  It is not known how this affect-s the results
of this correlation, although the effects would likely be small.
A positive correlation was also noted between the  growing season
epilimnetic total phosphorus concentration and the growing season
epilimnetic chlorophyll a (Figure 54).  While there are more data
sets for this correlation than for the relationship between an-
nual mean total phosphorus and growing season chlorophyll a_
(Figure 51), there is also more scatter of the data.  The cor-
relation between the growing season epilimnetic total phosphorus

                               244

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     Figure 48.  Mean Total  Phosphorus and Mean Secchi  Depth
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                                       ANNUAL MEAN  TOTAL PHOSPHORUS (mgP/l)
                              Figure 52.  Mean Total Phosphorus and  Growing  Season  Epilimnetlc
                                         Primary Productivity Relationship  in  US OECD  Water
                                         Bodies

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   GROWING SEASON  MEAN EPILIMNETIC TOTAL PHOSPHORUS (mg P/l)

Figure 54.   Growing Season Eplllmnetic Total Phosphorus and
            Growing Season Eplllmnetic Chlorophyll a Relationship
            1n US OECD Water Bodies

-------
and growing season primary productivity (Figure 55) is very similar
to that seen with the annual mean total phosphorus (Figure 52).
This suggests that the total phosphorus concentration does change
significantly over the annual cycle.  However, the scarcity of data
does not allow for a rigorous examination of these two relationships
A positive correlation was also noted between the spring overturn
total phosphorus concentration and the growing season epilimnetic
concentrations of chlorophyll a, total phosphorus and dissolved
phosphorus (Figures 56, 57 and 58, respectively).  However, none of
these three relationships had sufficient data for a valid assess-
ment of their degree of correlation.  It would particularly have
been informative to examine the correlation between the spring
overturn total phosphorus concentration and the growing season
chlorophyll concentration (Figure 56) since Sakamoto (1966), Dillon
and Rigler (1974a) and Vollenweider (1976a) have shown good corre-
lations between these two parameters.

     The correlation between spring overturn phosphorus and growing
season epilimnetic dissolved phosphorus was also examined (Figure
58).  Although there is somewhat of a positive correlation noted,
this is not a limnologically logical correlation to consider, since
the measured growing season epilimnetic dissolved phosphorus will
be the portion of the available phosphorus 'left over' in a water
body after the aquatic plant populations have assimilated their
metabolic requirements.  Consequently, the use of the 'available1
nutrients in any of the correlations is of dubious value.  They are
included in this analysis solely because they were included on the
initial list of suggested parameters supplied to all the OECD inves-
tigators .

     The mean dissolved phosphorus was also included in this
eutrophication response parameter analysis  but is not expected
to yield any useful correlations for the reasons indicated above.
There is a possible correlation between the mean dissolved phos-
phorus and mean chlorophyll a (Figure 59).  However, the mean
chlorophyll a is composed of annual mean, ice-free period mean and
surface mean~values.  Consequently, little validity was given to
this relationship.  By contrast, the correlation between the mean
total phosphorus and mean chlorophyll a (Figure 47) is much better
than that seen for mean dissolved phosphorus.  The correlation
between mean dissolved phosphorus and primary productivity
(Figure 60) partially supports this view.  There is considerable
scatter in the data for these two parameters which indicates
little correlation between them.  The primary productivity data is
too  scarce for correlation, but it is not expected that a larger
US OECD data set would show a positive correlation.

     There appears to be a positive correlation between mean
dissolved phosphorus and spring overturn dissolved phosphorus
(Figure 61).  This is not unexpected if the dissolved phosphorus
is the  'leftover1 fraction.  Presumably the larger the leftover
dissolved phosphorus content of the water body, the larger will
be the concentration at spring overturn.  However, this correla-
tion shows more data scatter than that between mean total phos-

                                  254

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                     OVERTURN TOTAL  PHOSPHORUS (mg P/l)
         Figure  56.   Spring Overturn  Total  Phosphorus and Growing
                     Season Epilimnetic  Chlorophyll  a Relationship
                     In  US OECO  Water Bodies

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     Figure  59.   Mean  Dissolved Phosphorus and Mean Chlorophyll  a_
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 phorus  and  spring overturn total phosphorus, as would be  expected.
 There also  appears to be no readily observable correlation between
 either  the  growing season epilimnetic dissolved phosphorus or the
 spring  overturn  dissolved phosphorus and the growing season
 epilimnetic chlorophyll a (Figures 62 and  63, respectively).  How-
 ever, the data set for these two correlations is very small, which
 precludes a rigorous analysis of these relationships.  It is
 possible a  positive correlation may exist  between the spring over-
 turn dissolved phosphorus concentration and the growing season
 epilimnetic chlorophyll a in phosphorus-limited water bodies.  A
 positive correlation is noted between the  spring overturn dissolved
 phosphorus  and the growing season epilimnetic dissolved phosphorus
 (Figure 64-).  However, in addition to a data set which is too
 small for a valid evaluation of this relationship, a positive
 correlation between these two parameters is not limnologically
 consistent  with  the conditions normally found in phosphorus-limited
 water bodies.  The available US OECD data  sets for these  two
 parameters  are almost completely for phosphorus-limited waters.
 Consequently, the apparent correlation is  probably an artifact.
 There were not sufficient data to examine the correlation  between
 spring  overturn  dissolved phosphorus and growing season epilimnetic
 primary productivity.  Presumably, if it existed, the correlation
'would be a  positive one.

 MEAN INORGANIC NITROGEN CONCENTRATIONS

     Before examination of correlations between the mean  inorganic
 (i.e.,  algal-available) nitrogen and eutrophication response
 parameters,  it should be noted that the concentrations of this
 algal nutrient,  as with dissolved phosphorus, will rise and fall
 as  a function of the algal activity in a water body.  Thus, as
 before, this nitrogen fraction will represent the  'leftover'
 nitrogen after the algal populations have  assimilated their
 stochiometric requirements for growth. Hence, an observed correla-
 tion may be an artifact of this process.   It is further complicated
 because the majority of the US OECD water  bodies are phosphorus-
 limited.  Therefore, the leftover inorganic nitrogen concentra-
 tions will  likely always be higher than the available dissolved
 phosphorus  concentration.  The same inorganic nitrogen forms were
 not reported for all US OECD water bodies.  Some investigators
 reported the mean concentration of NHiJ+NOj+NOJ (as N) and others
 reported NH^+NOg (as N), while still others reported NO^+NOJ (as N),
 These various combinations were treated as equal components in
 the correlations, although it is not correct to do so.  These
 factors should be considered when examining any correlations
 between inorganic nitrogen and other eutrophication response para-
 meters  in the US OECD water bodies.

     There  appears to be little correlation between mean  inorganic
 nitrogen and mean chlorophyll a_ (Figure 65).  Removal of  the one
 outlying point at low annual mean inorganic nitrogen and  chloro-
 phyll a results  in a situation in which there is essentially no
 relationship between the two parameters.   By contrast, there is a

                                  262

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                                                       PHOSPHORUS (mg/l)


                                   Figure 62.  Growing Season Epilimnetic Dissolved Phosphorus
                                               and Growing Season Epilimnetic Chlorophyll  a
                                               Relationship in US OECD Water Bodies

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                               SPRING OVERTURN  DISSOLVED  PHOSPHORUS (mg P/1)
                             Figure 63.   Spring Overturn Dissolved Phosphorus  and Growing
                                         Season Mean Epilimnetic  Chlorophyll  a Relationship
                                         i-n US OECD Water Bodies

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                                    ANNUAL MEAN INORGANIC NITROGEN (mgN/l)
                              Figure 65.   Mean Inorganic Nitrogen  and Mean Chlorophyll  a
                                         Relationship in US OECD  Mater Bodies

-------
good correlation between mean total phosphorus and mean chloro-
phyll a  (Figure 47).  This observation further substantiates the
importance of phosphorus, rather than nitrogen, in controlling
algal growth.  There is little or no correlation between mean inor-
ganic nitrogen and mean Secchi depth (Figure 66).  There is a
positive correlation between mean inorganic nitrogen and primary
productivity (Figure 67).  This further supports the phosphorus-
limitation of most US OECD water bodies.  If the water bodies were
nitrogen-limited, one would expect a negative correlation between
these two parameters.  In fact, the opposite correlation is
indicated in Figure 67.  By contrast, the poor correlation between
the dissolved phosphorus and the primary productivity (Figure 60)
illustrates its controlling role in the eutrophication process in
the majority of the US OECD water bodies.

     There is a correlation between mean inorganic nitrogen and
the growing season epilimnetic chlorophyll a (Figure 68).  A nega-
tive correlation would be expected if nitrogen were the controll-
ing algal nutrient.  Such a correlation was not seen in Figure 68.
A strong positive correlation appears to exist between the mean
inorganic nitrogen and the growing season epilimnetic primary
productivity (Figure 69).  However, there are only about a half
dozen data sets for this correlation.  This scarcity of data pre-
cludes any rigorous evaluation of this correlation.  The positive,
rather than negative, correlation suggests that nitrogen does not
control the algal populations.  The lack of data does not allow
one to evaluate the correlation between mean inorganic nitrogen
and spring overturn inorganic nitrogen.

     Interestingly, a negative correlation appears to exist be-
tween the growing season epilimnetic inorganic nitrogen and the
growing season epilimnetic chlorophyll a_ (Figure 70).  Although
the data set is somewhat limited, the correlation appears to be
real.  This indicates that, while phosphorus may control the algal
populations in most of the US OECD water bodies (Figure 62), the
need for available nitrogen for algal growth results in a de-
creased nitrogen concentration during the growing season.  A
positive correlation also appears to exist between the growing
season epilimnetic inorganic nitrogen and the growing season
epilimnetic primary productivity (Figure 71).  While the data
sets are relatively scarce for this correlation, it is consistent
with the views expressed above for Figure 70.

OTHER CORRELATIONS BETWEEN EUTROPHICATION RESPONSE PARAMETERS

     Several other correlations were also examined in this section,
as indicated in Table 26.  These latter correlations are grouped
together because they are of a varied nature.  They are discussed
below.   There is a positive correlation observed between the mean
chlorophyll a and primary productivity (Figure 72) in the US OECD
water bodiesT  Some scatter of the data is observed.  One would
normally expect a good correlation between these two parameters.
This is supported somewhat by the correlation between the primary


                                 267

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            Figure 68.  Mean Inorganic  Nitrogen  ar\d  Growing  Season  Epilimnetic
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        Figure 69.  Mean Inorganic Nitrogen and Primary Productivity
                  Relationship in US OECD Water Bodies

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-------
productivity and the mean Secchi depth (Figure 73).  As expected,
there is a negative correlation between these two parameters, al-
though the data are sparse.  However, the correlation exhibits a
considerable data  scatter, which limits its value.  It is noted
that a majority of the water bodies have primary productivities
ranging from about 40-1000 g C/m /yr, yet have Secchi depths
between 1-3 meters.

     A possible hyperbolic relationship was exhibited in the
correlation between mean chlorophyll a and mean Secchi depth.
The significance of this relationship as a "trigger" for public
response to eutrophic water bodies , and as a simple, practical
method for measuring water quality was discussed by Edmondson
(1972) and Carlson (1974).  This correlation is discussed in detail
in a later section of this report  (see Figures 77 and 78) and serves
as the basis of a nutrient load-water quality model developed in
this study.  There is a positive correlation between the growing
season epilimnetic chlorophyll a and primary productivity (Figure
74).  This is consistent with the observations indicated above
between the annual mean values of these two parameters.  There is
a somewhat better correlation between the growing season epilim-
netic values, as would be expected.  However, the very few data
sets limit the usefulness of this correlation as a predictive
tool.

     The annual mean primary productivity, on a daily basis, was
correlated with the annual mean chlorophyll a on both a volumetric
and areal basis (Figures 75 and 76, respectively).  This correla-
tion was analyzed solely because it appeared on the list of
suggested correlations.  There is a positive correlation between
the daily average primary productivity and the annual mean chloro-
phyll a concentration (Figure 75).   This correlation is similar
to that observed between the annual mean primary productivity and
annual mean chlorophyll a (Figure 72), except that the annual
primary productivity is expressed on a daily basis instead of an
annual basis.  Consequently, Figure 75 yields no more additional
information than is already noted in Figure 72.  There is little
or no correlation between the annual mean daily primary produc-
tivity and the annual mean areal chlorophyll a (Figure 76).   This
data set exhibits a considerable scatter.  There appears to be no
readily observable advantage in expressing mean chlorophyll a
concentrations on an areal basis instead of a volumetric basTs.

     In conclusion, there appear to be better correlations between
the phosphorus loads and concentrations of the US OECD water bodies
and the various eutrophication response parameters indicated above
than for the nitrogen loads and concentrations.  Consistent  with
phosphorus-limitation of the US OECD water bodies, there are
generally poor correlations between the dissolved (i.e., algal-
available)  phosphorus concentrations and the response parameters
examined in the US OECD water bodies.   While correlations also
existed between the nitrogen loads  and concentrations and response
parameters  of the US OECD water bodies , it is felt that many of
these correlations are coincidental artifacts caused by a relatively


                                275

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                                 ANNUAL  MEAN  DAILY  PRIMARY  PRODUCTIVITY (g C/m/day)
                              Figure  75.   Mean Daily Primary Productivity  and  Mean Chlorophyll a
                                          Relationship in US OECD Water  Bodies

-------
IVJ
CM
6
o»
_l
_J
i •
o
o:
3
X
o
1
__l
ut
cc.
z O.I
UJ
2
ZJ
z
z

I ' '
_ Invesligolor —Indicated
Trophic Slate:
• Eutrophic
A Mesotrophic
O Oligolrophic
[
A
A
0 • °.
- 0 »^A •
-
: A •
^_Q (0.002)
l i i i l i i il i i i > i i 1 1 1 i l l i i i i i
  0.01
O.I
10
ANNUAL  MEAN DAILY  PRIMARY  PRODUCTIVITY (gC/m  /day)
Figure  76.  Mean Daily Primary Productivity and  Mean Areal
           Chlorophyll a_ Relationship in US OECD Water Bodies

-------
constant N:P loading ratio and the basic phosphorus limitation of
the water bodies.   Several of these correlations,  notably total
phosphorus versus  chlorophyll a and chlorophyll a  versus Secchi
depth, have been used in the development of several phosphorus
load-water quality models presented in the following section of
this report.
                                280

-------
                            SECTION XI
     APPLICATION OF US OECD RESULTS FOR PREDICTING CHANGES IN
       WATER QUALITY AS A RESULT OF ALTERING NUTRIENT INPUTS


     It is of interest to attempt to predict the change in water
quality that might be expected to occur as a result of altering
the nutrient loading to a water body.  Attention will be focused
here on phosphorus loadings for reasons mentioned earlier; namely
because many US water bodies are phosphorus-limited, and because
phosphorus removal from point sources is both technically and
economically feasible (Vollenweider, 1968, 1975a; Lee, 1971, 1973;
Vallentyne, 1974; Vollenweider and Dillon, 1974).

     The specific question to be addressed is what is the change
in water quality expected from a change in the phosphorus load-
ings to a water body?  There are several ways to attempt to answer
this question.  The best overall approach that can be taken to
assess the effects of a change in phosphorus loadings on the
trophic conditions of a water body is based on the work of Vollen-
weider (1975a), discussed in an earlier section of this report.
Vollenweider's approach for assessing the degree of fertility of
a water body,  based on its phosphorus loadings and its mean depth
and hydraulic residence time characteristics, was presented graphi-
cally in Figure 19.  The results of the US OECD eutrophication
study, as well as those of the Canadian portion of the North Ameri-
can Project, and the Alpine, Nordic and Shallow Lakes and Reservoirs
Project have provided considerable support for this approach.  An
earlier version of this approach has also been used by tne US EPA
(1975a) in evaluating the phosphorus loading-eutrophication response
in the water bodies in the National Eutrophication Survey, as re-
flected in their degree of fertility.  Further5  this earlier version
was recommended by the US EPA in their Quality Criteria for Water
(US EPA,  1976a) as a basis for determining critical phosphorus load-
ings for US lakes and impoundments.

     As indicated earlier by examination of Figure 19, there is
remarkably good agreement between the overall trophic states of
the lakes and impoundments in the US OECD eutrophication study as
determined by their respective investigators and as indicated by
their phosphorus loadings and mean depth/hydraulic residence time
characteristics.  The US EPA, in the National Eutrophication Sur-
vey (US EPA, 1975a), has found a similar agreement for the water

                                 281

-------
bodies that they have investigated thus far.   In general, using
this relationship, it can be said that in terms of water quality
for a given set of morphologic and hydrologic characteristics,
as the phosphorus load is increased there is  a gradation of
deteriorated water quality, as measured by the frequency and
severity of obnoxious algal blooms.

     The reader should be reminded that the permissible and ex-
cessive lines on the Vollenweider phosphorus  loading diagram
(Figure 19) should not be interpreted as rigid values which de-
fine a certain level of water quality.  That  is, a water body
whose phosphorus loading and mean depth/hydraulic residence time
characteristics place it just above the excessive line should not
be rigidly viewed as having poor water quality.  Nor should a
water body plotting just below the permissible line be defined
strictly as possessing good water quality.  Rather, the influence
of eutrophication on water quality in a water body is dependent
on the public's response, as manifested in an impairment of use
of the water body.

     As discussed earlier, those water bodies with a given mean
depth/hydraulic residence time relationship which plot the great-
est vertical distance below the permissible boundary line can be
expected to have the best water quality.  Conversely, those which
plot the greatest vertical distance above the excessive loading
line would have the poorest water quality.  There is a continual
gradient of water quality between these two extremes, with the
permissible boundary area defining a general water quality con-
dition acceptable to most of the population.

     The position of these lines, as indicated in Equation 11, is
influenced by the work of Sawyer (1947).  While studying the ef-
fects of urban and agricultural runoff on the fertility of 17
lakes in southern Wisconsin, he found a 0.01 mg/1 phosphorus con-
centration in a water body at spring overturn to be a critical
concentration for high water quality.  Water bodies whose spring
overturn phosphorus concentrations exceeded this 0.01 mg P/l
critical concentration were likely to experience algal bloom
problems during the following summer growing season.  The Vollen-
weider model (Figure 19) is an extension of Sawyer's findings
which takes into account some of the morphological and hydrological
characteristics of a water body which influence its phosphorus
loading-algal growth relationships.

     The excessive and permissible phosphorus loading boundary
lines on the Vollenweider diagram are based mainly upon the recrea-
tional impact of eutrophication.  They do not address some of the
other parameters of water quality that are influenced by eutroph-
ication.  To cite one such example, one could not utilize these
phosphorus boundary loading lines to judge whether anoxic condi-
tions would develop in the hypolimnion of a water body.  A lake
could receive an excessive loading and still have an oxic

                                 282

-------
hyDolimnion throughout the year.  Dillon  (1975; Vollenweider
and Dillon, 1974; Dillon and Rigler, 1974b) has reported such
an occurrence in a number of water bodies in southern Ontario.
This occurred because hypolimnetic oxygen depletion, which is
an important eutrophication parameter, is dependent not only on
the nutrient load, but also on the hypolimnetic morphology
and the hydraulic flushing rate of the water body.  Furthermore,
the excessive and permissible phosphorus  loading lines in Figure
19 do not address the potential eutrophication problems arising
from excessive fertilization of water bodies used for domestic
water supplies (i.e., taste and odor problems, shortened filter
runs, etc.) as contrasted with recreational uses (Gaufin, 1964;
DeCosta and Laverty, 1964; Poston and Garnet, 1964; AWWA, 1966).

     While the positions of the US OECD water bodies in the
Vollenweider diagram (Figure 19) appear to be a good indication
of the overall eutrophication and associated water quality for
these water bodies, it is desirable to be able to translate this
relationship to a eutrophication parameter which is more easily
and widely appreciated by both the scientist and layman.  For
example, in Figure 19, the phosphorus loading to Lake Washington
decreased from 2.3 g/m /yr in 1964 (water body number 50 in
Figure 19), to 0.4 g/m^/yr in 1971 (number 51 in Figure 19),
moving it from the eutrophic zone to a position indicating a
much less productive water body.  However, a decrease in phos-
phorus loading or in-lake phosphorus concentration in a water
body does not necessarily mean that an improvement in water
quality has also occurred.  A concomitant change in a parameter
which is commonly used to indicate trophic conditions in a water
body would help one to appreciate the change in general water
quality resulting from a reduced phosphorus input.  This section
of this report presents the development and application of an ap-
proach for assessing changes in water quality to be expected
from a change in the phosphorus load to a water body.

     The first step in transforming phosphorus loading changes
to readily-appreciated indicators of changes in trophic conditions
is to examine the relationship between the spring overturn crit-
ical phosphorus concentration, and the average chlorophyll con-
centration during the following summer growing season.   Several
investigators (Sakamoto,  1966; Dillon and Rigler, 1974a,  1975;
Vollenweider, 1976a) have shown a strong relationship exists
between these two parameters.   Chlorophyll a concentration in
a water body is a much more readily observable consequence of
phosphorus loading than is a water body's phosphorus concentra-
tion.   The effects of phosphorus loading can be visibly appre-
ciated as a function of the resulting chlorophyll a concentra-
tion or "greenness" of a  water body.   Vollenweider~(1976a)  has
plotted chlorophyll a concentrations  as  a function of the phos-
phorus loading characteristics of a water body.   The theoretical
basis of this approach was presented  in  an earlier section of
this report.   The reader  is reminded  that the phosphorus  loading


                                283

-------
characteristic expression, ( (L(P) /qs) / (1+ 
-------
Washington, both before and after the completion of its ex-
tensive sewage diversion program.  The resultant chlorophyll a
changes tracked quite closely the expected changes (See Figure
9 in Vollenweider, 1976a).  It is noted, however, that a few
years after the sewage diversion project was completed, the
chlorophyll a concentrations tended to be higher than that
based on Vollenweider's relationship between phosphorus load-
ing and chlorophyll.  As discussed in an earlier section, this
is most probably related to the fact that a water body takes
several years to adjust to a new phosphorus loading.  This
time period of adjustment is equal to approximately three
phosphorus residence times (Sonzogni et_ al_. , 1976).  The
final result in Vollenweider's (I976a; application of the Lake
Washington data to his phosphorus loading and chlorophyll a rela-
tionship is in accordance with what is expected based on the
phosphorus load under equilibrium conditions.

     Thus, in summary, examination of the US OECD data as pre-
sented in Figure 22 indicates there is good agreement between
overall chlorophyll levels and phosphorus loads (as expressed^
in the phosphorus loading characteristics term (L(P)/qs)/(l+i
for water bodies studied in the US OECD eutrophication study.
This relationship will be used in the following pages to fur-
ther develop a meaningful relationship for assessing changes
in water quality to be expected following a change in the phos-
phorus load to a water body.

     The next  step in this effort is to examine the relation-
ship between chlorophyll and Secchi depth and then to unite
this relationship with the phosphorus load to a water body.  As
indicated in an earlier section, the use of the Secchi depth of
a water body as an indicator of its algal biomass and overall
water quality has been proposed by several investigators
(Edmondson, 1972; Carlson, 1974; Shapiro, 1975b; Shapiro et al. ,
1975).  Indeed, the Secchi depth is thought to be one of the
best overall parameters that the public could respond to for
improved water quality.  Edmondson (1972) and Shapiro et al.
(1975b) have presented similar conclusions on the value~of~the
Secchi depth as a measure of the impairment of water quality
by excessive fertilization.  A number of investigators have
demonstrated an inverse non-linear relationship between the
chlorophyll a content of a water body and its Secchi depth
(Edmondson, 1972; Carlson, 1974; Bachmann and Jones, 1974;
Dillon and Rigler, 1974a; 1974b; Dobson, 1975; Norvell and
Frink, 1975 and Michalski et  al., 1975).  Further,  the US OECD
data showed a similar relationship.  The pertinent  data are
presented in Table 27.   A plot of the chlorophyll a concentrations
and Secchi depths from these  various sources  is presented in
Figure 77.   The data reported by Edmondson (1972),  although
extensive and extending over  a number of years , was not in-
cluded in this plot since this relationship was for only one
water body while the other data sets were from a variety of
water bodies.   It was felt by these reviewers that  his data

                              285

-------
                             Table  27.   DATA FOR CHLOROPHYLL a AND  SECCHT  DEPTH RELATIONSHIP"
ro
oo
CD
Carlson
(1974)3
Chlor a
0
2
6
20
56
1
2
3
4
5
6
7
8
9
10
12
14
16
18
20
0
22
25
27
30
35
40
.94
.6
.4

















.5
.5
.0
.5
.0
.0
.0
b SD
8
4
2
I
0
7
4
0
3
2
2
2
1
1
1
1
1
1
1
1
12
0
0
0
0
0
0




.5
.7
. 8
.6
.0
.6
. 3
.0
.9
.7
.6
.4
.3
.2
.1
.0
.3
.90
.85
.80
.75
.70
.65
Dobson
(1975)a
Chlor a
5
5 ,
4 .
4 .
4 ,
4 .
4
3 ,
3
3.
1 .
1
1 .
0
1
0 ,











.9
. 2
. 8
. 8
.6
.2
.0
. 8
. 8
.6
. 3
.1
.1
.7
. 3
.7











C SD
2 ,
3 ,
2 .
2 .
2 ,
3 ,
3
5 .
4
6
7 .
8
8
7 .
7 .
8 ,











Dillon £
Rigler
O97'la)a
Chlor a° SD
.55
.18
.5
.4
. 8
.7
.75
.0
.6
.0
.2
.1
.7
.7
.9
.6











1
1
1
J
1
1
0
1
1
1
1
1
1
1
2
0
2
1
0
0
0
0
0
2
1
1
1
.6
.4
.2
. 3
.8
.1
. 8
.0
.1
.5
.2
.0
.8
.1
.1
.95
.7
.0
.4
.9
.4
.7
.7
.0
.3
.7
.4
(|
5
5
6
5
6
8
4
5
5
5
5
6
6
5
6
3
10
8
8
7
G
6
6
6
5
5
.3
.5
.9
.0
.5
.4
.7
.9
. 2
. 3
.5
.4
.2
.1
.9
.3
.5

.55
.1
.45
.85
.4
.1
.2
.95
. 7
Bachmann 6
Jones
(1974)
Chlor a
4
5
1
3
5
4
3
4
8
4
5
6
6
9
12
9
10
10
12
10
22
30
14
23
14
24

.5
.5
.5
.0
.0
.0
.5
.5
.0
.5
.0
.0
.0
.0
.0
.5
.0
.0
.5
.5
.5
.5
.0
.0
.5
.0

SD
5
5
4
3
3
3
3
2
2
Z_
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1

.5
.1
.75
. 7
.5
.45
.0
.9
.9
.85
.5
.5
.4
.3
.1
.05
.9
.8
.7
.65
.6
.45
.2
.1
.0
.0

Norvell £
Frink
(1975)
Chlor a SD
0
0
50
30
14
7
13
34
2
7
a
0
1
4
9
4
7
38
5
2
2
4
5
14
13
1
3
.6
.9



.2
.9

.4
.5
.6
.G
.5
.8
.0
.8
.8

.2
.8
.0
.2
.6
.3
.8
.7
.2
6
8
1
1
2
4
2
2
5
4
5
5
6
5
3
4
3
1
3
4
4
3
4
2
3
4
4
.3
.2
.8
.5
.2
.0
.5
.0
.7
.5
.3
.0
.0
.0
.5
.8
.3
.9
.5
.8
.5
.8
.0
.5
.0
.5
.3
US OKCD Eu-
trophication
Study3
Chlor a
14.
5 .
6 .
6 .
5 .
6
5
7 .
5 .
20e
26.
G
4
33.
3.
53d
13.
21.
6
3
5
2
10
5
21
12
10
6d
gO
°d
3d
0


4
6
c\
5

A
9d
5

2
2









SD
3
1
2
2
1
2
2
1
2
1
1
2
2
0
2
1
1
1
1
2
3
3
3
2
1
1
1
.6
.5
.1
.0
.7
. 3
. 3
. 8
.4
. 8
.5
.7
.5
.8
.4
.0
.4
.2
.8
.2
.1
.0

.3
.5
.8
.8
Michalsk
et al .
(1975)
Chlor
1
2
1
2
7
8
6
13
6
4
9
14
5
14
20
24
18










.1
.2
.0
.3
.2
.0
.8
.5
.6
.1
. 3
.1
.8
.4
.0
.9
.0










a
8
6
4
4
J
3
3
2
2
2
2
1
1
1
1
0
0










i
a
SD
.05
.25
.35
. 0
. 35
.15
.0
.65
.5
.1
.1
.95
. 80
.1
.1
.85
.45











-------
                           Table  27  (continued).   DATA  FOR CHLOROPHYLL a AMD SECCHI DEPTH RELATIONSHIP0
ro
oo

Dillon £
Carlson Dobson Riglor
(1974)3 (1975)3 (197i|a)a
Bachmann £ Norvell &
Jones Frink
(1974) (1975)
Clilor ab SD Chlor a° SD Chlor a° SD Chlor a
45.0 0 . G 0 2
50.0 0 . 5 5 0
60.0 0.50 1
70.0 0.45 1
|
1
2
2
6
1
]
2
1
3
1
7
1
0
1
1
2
3
2
]
2
1
1.
.4
.5
.5
. 5
. 5
.7
. 2
. 2
.6
.2
. 8
.4
.0
.4
. 3
. 2
. 8
. 8
.'I
.8
. ^
. 3
. 7
. 7
. 8
.'!
. 5
5
5
5
c
5
5
5
4
4
4
It
4
4
4
4
4
4
4
^
4
3
3
3
3
3
3
3
. 7
r
. 5
. 35
.?b
.25
.25
.95
. 7
. 7
. 7
. 7
.55
s s
.45
.35
. 35
.25
. ?5
.1
.95
. 85
. 7 5
. 7
. b
.55
.45
SD Chlor a SD
1 5
3
2
3
31
9
5
5
r
2
13
9
1
1
0
2
103
25









.5
.2
.3
.2

.9
.9
. 3
.5
.4
.]
.0
.8
.2
. 7
.4











2
6
7
6
2
3
4
3
3
6
2
3
6
7
7
8
1
2









.0
.8
.5
.0
.5
.0
.5
.5
.3
.0
. 3
.2
.8
.2
. 3
.2
.0
.5









US OECD Eu- Michalski
trophication et al.
Study3 Tl975)a
Chlor a
90
65
15
12 .8
-------
                     Table 27  (continued).   DATA  FOR CHLOROPHYLL a AND SECCHI DEPTH RELATIONSHIP11
CD
CO

Dillon &
Carlson Dobson Rigler
(197i4)a (1975)3 (1974a)a
Chlor ab SD Chlor ac SD Chlor ac SD
2
?
3
5
7
I
4
9
6
5
13
17
3
19
14
14
7
13
16
.9
.2
. 2
.4
.9
.7
.9
.0
.1
.4
.2
.9
.9
.6
.5
.9
.5
.1
.3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
0
0
Bachmann 6 Norvell E US OECD Eu- Michalski
Jones Frink trophication et al.
(1974)a (1975)a Study3 ~U975)a
Chlor a SD Chlor a SD Chlor a SD Chlor a SD
.45
.4
. 35
.65
.35
.25
.2
.15
.15
.0
.9
. 7
.4
.2
.2
.05
.0
. 75
.45
           Explanation:
             b
Source of data; all chlorophyll values are in pg/1;  all  Secchi  depth values are in meters

Surface chlorophyll
             d
Summer total chlorophyll

Upper 2 meter's of water column
              Summer surface moan  values

-------
   9.0
8.5 F



8.0



7.5



7.0



6.5
X
K
0-
X
o
o
LJ
CO
6.0



5.5



5.0 |a



4.5



4.0



3.5



3.0



2.5



2.0



1.5



1.0



0.5
      Cf
       •"on
       OD
           on
      -  6  D
         i<>£»  n
          0 tl
          5  x°
            x00  D
         *3&      m
       0*S%°0   D.
        •-I  I sD
                         D     D

                      •     •
          C& 0 "   n

         *-•  S.A°    o.
          *^  ^ %        0  •*   '
                A.*   •«    »  R>

                  §3 A  O • .     •
                                                  DATA FROM:

                                               A CARLSON (1974)

                                               V DOBSON1I976)

                                               O DILLON AND RI6LER (19740,1975)

                                               0 BACHMANN AND JONES (1974)
                                               D NORVELL AND  FRINK (1975)

                                               X MICHALSKI «!  OUI975)

                                               • US OECD STUDY
                                                        _L
                                                             J_
                                                                           _i_
              10   15
                         25   30   35   40   45   50   55   60   65   70   75   80  • 85   90


                                MEAN CHLOROPHYLL a (ug/D
     Figure  77.    Secchi  Depth and  Chlorophyll  a_ Relationship  in
                      Natural   Waters  (Linear  Scale).
                                         289

-------
base would bias the resultant plot toward the chlorophyll a_ and
Secchi depth relationship typical of Lake Washington.  In addition,
an examination of the Secchi depth and chlorophyll relationship in
Lake Washington showed it to be somewhat different than that seen
with the other sources listed above.  Consequently, only data re-
ported from a wide range of water body types were used in Figure 77.
However, a comparison of this figure with the plot presented by
Edmondson (1972) shows a similar hyperbolic relationship
between these two parameters, with the slope of the curve steepest
at the lower chlorophyll concentrations.

     As it is difficult to get an accurate regression line of best
fit for the non-linear chlorophyll a and Secchi depth relationship
illustration in Figure 77, the same~data sets were plotted on a
double logarithm plot.  This is illustrated in Figure 78.  The
regression equation for this plot is:

  Iog10 Secchi depth = -0.473 log1Q  [chlorophyll a] + 0.803

                                                          (39)

The regression line has a correlation coefficient, r = -0.85, in-
dicating a good correlation between the chlorophyll a_ content and
Secchi depth in natural waters for a wide variety of water bodies
located throughout the US.

     Since the chlorophyll content of a water body is related to
its phosphorus loading characteristics  (Figure 22), and since
a strong correlation was demonstrated above between chlorophyll a
and Secchi depth (Figure  78), there  should also be a relationship
between a water body's phosphorus loading characteristics and its
Secchi depth.  In fact, the final step remaining in this exercise
is to unite both these relationships (Figures 22 and 78) into a
single expression which directly relates these two parameters.
This has been accomplished by producing a double logarithmic plot
of the phosphorus loading expression, (L(P)/qs)/ (l+,/!2T/qs) , and
Secchi depth, as illustrated in Figure  79.  The line of best fit
was extrapolated from the data presented in Figures 22 and 78.
Chlorophyll a. values, as a function  of a water body's phosphorus
loading characteristics, were taken  from Figure 22.  Then, the
expected Secchi depth for a given chlorophyll a_ concentration was
taken from Figure 78.  The expected  Secchi depth was then plotted
as a function of the original phosphorus loading expression above,
to produce the line of best fit illustrated in Figure 79.  Using
least square analysis, the regression equation for this line is:

  Iog10 Secchi depth = -0.359 Iogl0  [ (L(P)/qs ) / (1+,/fTq^) ] + 0.925

                                                          (40)

Using this relationship  (Figure 79), one can determine the Secchi
depth to be expected as a function of the phosphorus loading


                                 290

-------
                  20
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                  10
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X
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                                                    o
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                                     Source of Polo:

                                    Corlson(l974)
                                    Dobson (I97S)

                                    Dillon 8 Rigler (I974o ; 1975)

                                    Bochmonn 8 Jones (1974)

                                    Michalski et oM(975)
                                    Norvell 8 Frink (1975)
                                    US OECD Study
                          log Secchi depth = -0.473 log [Chlorophyll o] 40.803
                 0.4
                                  I
                                                     I
                                                                                   1     1   1   1  1
                   0.4
I                                  10

      CHLOROPHYLL g CONCENTRATION (/Ltg/l)
                       Figure  78.
                                                                                      100
                       Secchi  Depth  and  Chlorophyll  a^  Relationship  in
                       Natural  Waters (Log-Log  Scale).

-------
z&z
MEAN SECCHI DEPTH (meters)
o
In - C
- \ /[(L(P)/qs)/(l+.y?/qs)]=4.0(36); 1.8(48)
| Secchi depth = 28.3(36), 28(48)
L tog Secchi deplh = -0.359 log[(L(P)/qs)/(l+/fAts)|+0.925
— -«^. ... ¥ ,, | INVESTIGATOR-INDICATED
-~-v^^* ^ • TROPHIC STATE;
. O ^^"^^ ~ 44 ^2 •-EUTROPHIC
lOT "-^^. 45 « ,3 •
°)9 ->>. • 40 34 4J « A-MESOTROPHIC
° 26 49^>>^ *4 ^ O-OLIGOTROPHIC
• ^•^"•^^^•••^8
• l6 54 ^-vAe «35
50 • 'V^-^ 47
(US OECD woler bodies ore • *^ — „
plotted obove) ^
28
i i I t I I I I I I I I I 1 i I I I I I I 1

> 10 100 1000
                           (mg/m3)


Figure  79.   Phosphorus Loading Characteristics  and  Secchi
            Depth Relationship in Natural  Waters

-------
characteristics of a given water body.  As indicated earlier,
this relationship allows one to be able to determine the change
in water quality in a water body, expressed as a function of
its Secchi depth, which would result from a change in its
phosphorus load.  The change may be deterioration or enhancement
of water quality (i.e., decrease or increase in Secchi depth)
depending on whether the phosphorus flux to a water body was
increased or decreased.  This relationship, therefore, represents
a single, practical application of some of the results of the
US OECD Project in assessing the effects of phosphorus loadings
to water bodies as expressed as a function of a widely-appreciated
parameter of eutrophication, both to scientists and laymen.

     There are several precautions that should be noted in the
use of this relationship.   One consideration is that it would
hold only for those water bodies where the primary factor con-
trolling water clarity is phytoplankton.  It would not be ap-
plicable in its present form to water bodies with large amounts
of inorganic turbidity or color.  However, it may be possible to
partially correct for the effects of excessive inorganic turbidity
and color on the Secchi depth of a water body.  According to
Vollenweider (1977), in the simple case, the Secchi depth may be
computed as the integral of the turbidity above the Secchi disk
(i.e., / T(g)da = constant, where S = Secchi depth (m), T(B) =
mineral turbidity at depth g (mg/1), and T(g) is inhomogenous
over depth g).   For the homogenous case, the Secchi depth may
be calculated as S = l/(k]_ + k2 (C) + k3 (T) + k4 (Chi)), where
C = color (mg P,/l), T = mineral turbidity (mg/l), Chi = chlorophyll
a (mg/m3) and k,, k2, k  and k. = constants.  Vollenweider (1977)
is evaluating trie constants as follows:  k-, * 0.025, k~ ~ 0.005 to
0.01 and k^ * 0.01 to 0.02.  The constant k"3 is difficult to
estimate because of the lack of appropriate data for expression
of the interaction of primarily biological turbidity with chlor-
ophyll a.  In relatively transparent water (i.e., little color
or mineral turbidity), one may approximate k~ by use of the
relationship, S = l/(k«(Chl) .   For very transparent waters, C,
T and Chi can be expected to be very small.  Accordingly, recalling
k^ ~ 0.025, the Secchi depth approximates 40 meters (i.e., S =
1/0.025).  For less transparent waters, one will have a family
of curves, depending mainly on the terms k? (C) and k~(T).  Thus,
one could attempt to correct the Secchi depth for high color or
inorganic turbidity in water bodies using the relationships
expressed above.  The corrected Secchi depth can likely then be
applied in the previously-mentioned equations relating the phos-
phorus loading, chlorophyll a and Secchi depths in natural waters.
It should be noted, however, that it was not possible to test the
homogenous equation above because of lack of sufficient data for
the US OECD water bodies.   Few water bodies in the US OECD eutro-
phication study had excessive color or turbidity to permit such
an evaluation.

     This relationship would also not hold for water bodies
whose excessive phosphorus loadings were manifested principally


                               293

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in excessive macrophyte growths and attached algae,  rather than
in nuisance planktonic algal blooms.   Such water bodies tend
to have a larger Secchi depth than would be expected on the
basis of phosphorus loadings alone since a portion of the phos-
phorus input would be incorporated into the macrophytes rather
than into the phytoplankton.  Finally, this relationship would
hold only for those water bodies whose phosphorus loadings were
relatively constant (i.e., in an equilibrium state).  This is
because the phytoplankton populations and, hence, chlorophyll
content of a water body, are a function of the phosphorus concen-
tration, which in turn is a function of the phosphorus loading
to the water body.  The relationship between the phosphorus con-
centration in a water body and the phosphorus load to the water
body is a complicated one (Vollenweider, 1968), being a function
of the water body's mean, depth, hydraulic residence time,_in-
ternal loading, aquatic plant population, etc.  However, if the
phosphorus load is relatively constant over the annual cycle, it
can be expected that the mean total phosphorus concentration is
also relatively constant over the annual cycle.  Under such
equilibrium conditions, the use of Figure 79 to predict Secchi
depth as a function of a water body's phosphorus loading character-
istics should present no problems.  On the other hand, if the
phosphorus load to a water body is increased or decreased signifi-
cantly, as in a sewage diversion project or the introduction of
sewage treatment plant effluent to a water body, then the rela-
tionship expressed in Figure 79 would likely not be valid for
prediction of Secchi depth.  As discussed by Sonzogni et al.
(1976), a water body does not instantaneously adjust to a new
phosphorus load.  Rather, a period of approximately three times
the phosphorus residence time is necessary for a water body to
adjust to a new phosphorus load.  After this time period, assuming
the phosphorus load has not been further changed since an initial
increase or decrease, one could expect to again be able to use
Figure 79 to predict Secchi depth in a water body as a function
of its phosphorus load characteristics.  It should be noted
that this represents a simple, quantitative and practical method-
ology  for determining what the expected Secchi depth will be in
a water body in response to a sewage diversion or advanced treat-
ment project, prior to initiation of the project.

     If one examines the phosphorus loading and Secchi depth data
for Lake Washington (Vollenweider, 1976a; Edmondson, 1975a), the
1964 phosphorus loading for Lake Washington,  at the  -initiation
of its sewage diversion project, gives it an  (L(P)/qs)/(l+/z/qs)
value of approximately 100  (Vollenweider, 1976a).  This corresponds
to a Secchi depth of about 1.6 m.  Edmondson reported a mean
Secchi depth for Lake Washington in 1964- to be 1.2 m.  However,
the phosphorus loading, had been increasing dramatically since 1957
(i.e.,  (L(P)/qs)/(l+/z/qs) value of approximately 40 in 1957 and
1964 value of 100), and consequently the relatively poor prediction of
Secchi depth was not unexpected.  However, as noted earlier in
Table 21, the phosphorus residence time for Lake Washington was
approximately one year in 1964.  Thus, according to Sonzogni et al.
(1976), one could expect a new phosphorus concentration equilibrium

                                  294

-------
condition in about three years.  However, the sewage diversion
project, although begun in the early 1960Ts, was not completed
until about 1968.  Therefore, by 1972-1973 at the latest, one
could expect phosphorus equilibrium conditions to again exist.

     In fact, if one examines the phosphorus loading expression
value for Lake Washington in 1971 and 1974  (Vollenweider, 1976a)
and compares the Secchi depth predicted in  Figure 79 (i.e., 3.7 m
and 3.5 m, respectively) with the mean Secchi depth reported by
Edmondson (i.e., 3.5 m and 3.8 m, respectively), they are quite
similar.  The small discrepancies may exist because Vollenweider
(1976a) appeared to use slightly different  phosphorus loadings in
his Lake Washington calculations than those reported by Edmondson
(I975a), at least for 1971.  If one uses the phosphorus loadings
reported by Edmondson in 1971 in Figure 79, the predicted and
reported Secchi depths for that year are identical.  Edmondson
(1975a) did not report phosphorus loadings  for 1974, so it was
not possible to compare the predicted and observed Secchi depths
for that year based on his loadings.  For this reason, the
(L(P)/qs)/(l+/z/qs) expression values indicated by Vollenweider
(1976a) were used to compare the predicted  and observed Secchi
depth values for 1971 and 1974.  Even so, the agreement between
these two Secchi depth values for 1971 and  1974 is quite good,
lending support to this approach in assessing water quality as
a function of several easily understood and measurable parameters.
It should be noted that, as was the case for the phosphorus load-
ing characteristic and chlorophyll a concentration relationship,
this new relationship also indicates that a relatively large
change in the phosphorus load must occur to water bodies in order
to show marked improvement in water clarity.

     It is also feasible to develop a model which relates pnos-
phorus loads to the water quality parameter of hypolimnetic
oxygen depletion.  This latter parameter is of concern because
of its implications for the development of anoxic conditions in
hypolimnetic waters, especially in eutrophic water bodies.  The
consequences of anoxic conditions in the hypolimnion on the cold
water fisheries which usually populate this region of a water
body are obvious.  The chemically-reducing conditions usually
found in an anoxic hypolimnion also have implications for water
quality.  For these reasons,  the development of a water quality
model relating phosphorus loads to hypolimnetic oxygen depletion
is discussed below.

     Gilbertson e_t al.  (1972) found a remarkably good linear
correlation between municipal phosphorus loads and hypolimnetic
oxygen depletion rates in the central basin of Lake Erie.  Based
on the observed period of thermal stratification and the oxygen
levels in Lake Erie's central basin, Gilbertson e_t al.  determined
that the critical oxygen depletion rate in the hypoTTmnion of Lake
Erie's central basin was about 2.7 mg 02/l/month.   That is,  a
hypolimnetic oxygen depletion rate of 2.7 mg 02/I/month during the
period of thermal stratification would produce a zero concentration


                               295

-------
of oxygen in the hypolimnion of the central basin of Lake Erie
by the end of a given summer.  Examination of the historical
data for Lake Erie (Gilbertson e_t al. , 1972) indicates this
critical depletion rate corresponds~to the 1955 phosphorus loading
conditions of about 12,000 tons  per year, and has been exceeded
every year since that time.

     The observations of Gilbertson et al. suggest that a gen-
eralized approach relating phosphorus~loads and hypolimnetic oxygen
depletion would appear to be feasible for a wide range of water
bodies.  One approach for developing such a relationship involves
the use of a model derived by Lasenby (1975) between areal hypo-
limnetic oxygen depletion and Secchi depth.  Studying 14 lakes
in southern Ontario, and several other water bodies Lasenby re-
ported that a strong inverse relationship (r=-0.85) appeared to
exist between the areal hypolimnetic oxygen depletion rate and
Secchi depth in these water bodies, as follows:
                                                             2
     log, n areal hypolimnetic oxygen depletion rate (mg 0,.,/cm /day)

         = -1.37 log1Q Secchi depth (m) -0.65                 (41)

An assumption in Lasenby 's model was that the quantity of seston
sinking into the hypolimnion was proportional to the quantity in
the epilimnion.  Lasenby (1975) has indicated that the linear
development of his hypolimnetic oxygen depletion model suggests
that hypolimnetic oxygen consumption is not too sensitive to brief
changes in productivity and, therefore, relatively few measurements
should give a good estimate of oxygen depletion rates.

     With the relationship expressed earlier between phosphorus
loading and growing season mean Secchi depth (Equation 40), and
using Secchi depth as the common variable, Equation 41 above was
used to derive a relationship between phosphorus loading and areal
hypolimentic oxygen depletion.  This model was then tested using
US OECD data, as well as data presented by Welch and Perkins
(1977) for a large number of water bodies with a wide range of
trophic conditions.  Examination of the data indicated that Lasenby 's
relationship, derived mainly from oligotrophic and mesotrophic
water bodies, tended to overestimate the areal hypolimnetic oxygen
depletion rates in the majority of the water bodies.  Consequently,
it was decided to use simple linear regression techniques, as was
done with Figure 22, to determine the best relationship.  The
following regression was obtained:
                                                           2
     log, n areal hypolimnetic oxygen depletion rate (g O^/m /day)
      = 0.467 log, n [(L(P)/q )/(l+z/q )] -1.07            (42)
                 J_ U         S          S

This relationship is illustrated in Figure 80, along with the
available US OECD data, as well as data furnished by Welch and


                               236

-------
Perkins  (1977) for several other water bodies.  The model refers
to the mean areal hypolimnetic oxygen depletion rate during the
period of thermal stratification.  Since the oxygen depletion rate
is expressed on an areal basis, it can be applied to any hypo-
limnetic volume, regardless of size pr oxygen content.  It should
be noted that the units of hypolimnetic oxygen depletion in Equa-
tion 42 are different from those presented in Equation 41.

     Very few studies on hypolimnetic oxygen depletion were con-
ducted on the US OECD water bodies.  Consequently, the data base
for testing this phosphorus load-hypolimnetic oxygen depletion
model (Equation 42) was not as large as that for either the phos-
phorus load and chlorophyll a. or Secchi depth models.  Although
there is some scatter of the data in Figure 80, considering the
uncertainty in the data available for the phosphorus loads, hypo-
limnetic volume, area and oxygen concentration, thermal stratifi-
cation, etc., the agreement between the predicted and observed
values is reasonably good and provides support for this model
as a predictive management tool for assessing the effects of a
given phosphorus load on the hypolimnetic oxygen depletion in a
water body.  Further details concerning this model are presented
in Rast  (1977).
APPLICATION OF RESULTS FOR ASSESSING WATER QUALITY IN LAKES AND
IMPOUNDMENTS

     The approach presented  in this  section of this report can be
used to assess the potential effects of phosphorus load reductions
on water quality in lakes and impoundments.  Assessments of this
type are becoming increasingly important  in developing the most
cost-effective phosphorus control  strategies for these water bodies.
In the past,  eutrophication  control  strategies were frequently based
on the removal of phosphorus from  its most readily controllable
sources, without any  quantitative  assessment possible beforehand of
the magnitude of water quality improvement that would result from
controlling the phosphorus input to  a certain degree.  The implemen-
tation of  Section 314-A of PL 92-500 will require water pollution
regulatory agencies throughout the US to  develop nutrient  control
strategies for those  water bodies  which are found to be excessively
fertile.   As  a result of the US OECD eutrophication program, it
will now be possible  for these agencies to quantitatively  assess
the magnitude of water quality improvement that can be achieved
as a result of a phosphorus  input  reduction of a certain amount.
This section  of this  report  discusses the application of these
results to a  hypothetical situation  which is likely to be  typical


                                 297

-------
r-O
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-
log oreol

H— CENTRAL

O
hypolimnetic oxygen depletion (gOo/171 /day)
0.467 log[(L(P)/qs)/(l + v/r;7')]- 1-07

BASIN OF LAKE ERIE
( Year of Data Record )
• — US OECD WATER BODIES
(See Table
14 For Identification Key)
A — WASHINGTON, USA, AND ONTARIO, 03 ^*
CANADA, WATER BODIES ^*
(Data Taken From, And Water Bodies 34 ^•^^
Identified

—
-
-
-



A
.^
^ ,
In, Welch And Perkins, 1977) ^ • ^>*^
' D ^^
• * 4I 13 ^* 	 ~
• ^
45 441^9
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A ^AT^^dgei) _
A A *r^ m "
**f£ • (1966)
^^^^ m (1956)
^•^^ ^^ A A
^^^^ 1 I Q * 1 \ ^^^_ ^& ^K
^^^ \ i y ^ i / ^^^B A^ / 1 Q ^ 1 1 £A
(1931) • A
A(0.06) A
i i if i ill I i i i i i i i 1 i i i i i i i i
                                100
              Figure 80.
                                   10
                 ( mg/m3)

Phosphorus  Loading Characteristics and Hypolimnetic
Oxygen  Depletion Relationship in Natural  Waters
1000

-------
of what pollution control agencies will encounter as they attempt
to implement Section 314-A of PL 92-500.  The approach taken in
this section is patterned after the approach developed by Rast
(1977) and used by Lee (1976) and Lee et_ al. (1977) to assess the
improvement in water quality that would occur in the Great Lakes as
a result of a phosphate detergent ban in the State of Michigan.

     A hypothetical phosphorus loading situation has been con-
ceived in this section to illustrate the use of the approach des-
cribed above for assessing the potential effects of phosphorus
load reductions on water quality in a water body.  Several phos-
phorus load reduction possibilities are considered.  The phosphorus
loads and other pertinent data for analyzing the potential effects
of phosphorus load reductions are summarized in Table 28.  In this
hypothetical water body, the point source inputs are 56 percent of
the total phosphorus load, with non-point sources comprising the
other 44 percent.  The initial phosphorus loading is the hypo-
thetical load for 1975 and consists of both point sources (domestic
wastewater treatment plants) and non-point sources (land runoff
and atmospheric inputs).  As shown in Table 28, in this hypothet-
ical example, the point source phosphorus load is 6.6 million
kg/yr, while the nonpoint phosphorus load is 6.2 million kg/yr.
For an assumed surface area of 2.6 X lO^O m?, this corresponds
to an areal loading of 0.46 g P/m^/yr.  The first modified phos-
phorus loading considers the effects of a detergent phosphate ban
on the loading to the water body.  It was determined by Lee (1976)
that a detergent phosphate ban would result in approximately a 35
percent reduction in the amount of phosphorus in domestic waste-
waters.  This percentage value was used in these examples.  This
would reduce the phosphorus input to the hypothetical water body
from this source by the same magnitude.  This reduction would change
      •                                       C                  R
the point source phosphorus load from 6.6 x 10" kg/yr to 4.3 x 10
kg/yr, and reduce the overall areal load from 0.46 to 0.37 g P/m /yr

     The second modified condition considers the effects of a 90
percent phosphorus removal from the domestic wastewater treat-
ment plant loadings.  In this case, it will simulate the effects
of advanced waste treatment for phosphorus removal on the point
source load to the water body.  The 90 percent removal reduces the
point source phosphorus load to 6.6 x 10"3 kg/yr, and the overall
areal load to 0.23 g P/m^/yr.  The third modified phosphorus load-
ing simulates the effect of advanced waste treatment on the sewage
treatment plant inputs plus phosphorus loading reduction from the
non-point sources.  In this case, it will simulate the effects of
advanced waste treatment phosphorus removal techniques on land
runoff.

     Figure 81 presents the Vollenweider phosphorus load and mean
depth/hydraulic residence time relationship for this hypothetical
water body.  Included in Figure 81 are the expected changes in
phosphorus loading for each of the phosphorus loading reduction
scenarios described above.  Examination of Figure 81 shows that
the phosphorus load for 1975 places the water body in the eutrophic

                                 299

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      Table 28.  SUMMARY OF DATA FOR HYPOTHETICAL
                 WATER BODY UNDER SEVERAL PHOSPHORUS
                 LOAD REDUCTION SCENARIOS
A)  Morphometric and Hydrologic Data:
    1)  Volume = 4.55 x 10i:Lm3
                                in  9
    2)  Surface area =  2.6 x 10   m
    3)  Mean depth (volume/surface area) = 17.7 m
    4)  Hydraulic residence time (volume/annual inflow volume) =
        2.6 yr.
    5)  Phosphorus residence time (phosphorus content/phosphorus
        load) = 0.56 yr.

B)  Phosphorus Loading Data:
    1)  1975 phosphorus load -
        a)  point sources3:  ,                  6.6  x 10,. kg/yr
        b)  non-point sources :                 5.2  x_10  kg/yr
                                total load =   1.2  x 1C7 kg/yr
                                           ~   0 .46 g P/m2/yr
    2)  1975 phosphorus load minus detergent phosphate -
        a)  point sources3:  ,                  4.3  x 10_ kg/yr
        b)  non-point sources :                 5.2  x 10  kg/yr
                                total load =   9.5  x 106 kg/yr
                                               0.37 g P/m2/yr
    3)  1975 phosphorus load minus 90 percent point
        source loading -
        a)  point sources3;  ,                  6.6  x 10  kg/yr
        b)  non-point sources :                 5.2^  x 10  kg/yr
                                total load =   5.8  x 10  kg/yr
                                               0.23 g P/m2/yr
    4)  1975 phosphorus load minus 90 percent point
        source loading minus 40 percent non-point
        source loading -
        a)  point sources3:  ,                  6,6  x 10g kg/yr
        b)  non-point sources :                 3^.1_ x 10  kg/yr
                                total load =   3.7  x 10  kg/yr
                                               0.15 g P/m2/yr
Explanation:
aassumed to consist solely of sewage treatment plant inputs.
'-'includes atmospheric inputs.
                                 300

-------
   10
         EUTROPHIC
           ZONE
                                                                  EXCESSIVE
                                                                 /LOADING
                                                                  PERMISSIBLE
                                                                  /LOADING
I
o
z
o
_J
tr
o  o.i
i
Q.
ir>
O
I
0.
1975 P-Lood Minus
Detergent P-Lood
                                                 1975 P-Lood Minus 90 Percent
                           1975 P-Lood
                             1975 P-Lood Minus 90 Percent
                             Point Source P-Lood  ond
                             40 Percent Nonpoint Source
                             P-Lood
                                                            OLIGOTROPHIC
                                                                ZONE
  0.01
                                              _L
                                                  I  I I  I I III
                                                 J	I	I I  I III
    O.I
       I                  10
MEAN DEPTH,z/HYDRAULIC RESIDENCE
                   (m/yr)
                                           100
                                                                          1000
Figure 81.   Phosphorus  Loading  and  Mean  Depth/Hydraulic Residence
              Time  Relationship  as Applied  to Hypothetical Water  Body
              Under  Several  Phosphorus  Loading Scenarios
                                     301

-------
zone of the Vollenweider phosphorus loading diagram, based on
the water body's mean depth and hydraulic residence time charac-
teristics.  When the detergent phosphate ban is considered,
there is a discernible decrease in the phosphorus load (e.g.
approximately 20 percent decrease in total phosphorus load),
as indicated in Figure 81.   It is important to note that Figure
80 is based on total phosphorus loadings to the hypothetical
water body, which may not  properly reflect the phosphorus in-
put which is available for utilization by the phytoplankton
populations in the water body.  It is reasonable to suggest
that the decrease in the available phosphorus fraction of the
phosphorus load to the hypothetical water body will be somewhat
less than shown in Figure  81,.

     If the point source load is reduced by 90 percent, as
would be seen with advanced waste treatment phosphorus removal
techniques, there is a relatively large decrease of approximately
50 percent in the total phosphorus load.  This would place the
hypothetical water body in the mesotrophic zone of the Vollen-
weider diagram, based on its mean depth and hydraulic residence
time characteristics.  The reduction of the phosphorus input
from non-point sources can potentially be achieved by a variety
of means such as control of agricultural use of fertilizers and
animal manures, improved street sweeping to minimize phosphorus
derived from urban drainage, and/or the control of atmospheric
inputs of phosphorus.  As  shown in Table 28, a 90 percent point
source and 40 percent nonpoint source phosphorus removal program
reduces the areal phosphorus load to 0.15 g P/m^/yr.  The impact
of advanced waste treatment for point source phosphorus removal,
plus control of the diffuse sources, places the hypothetical water
body in the oligotrophic zone of the Vollenweider phosphorus load-
ing diagram (Figure 81).  As discussed in another section of this
report, it is important to emphasize that a change in the position
of a water body, based on an altered phosphorus load, from just
above, or just below, the excessive and permissible loading lines
in a Vollenweider phosphorus loading diagram does not necessarily
translate into a significant change in water quality.  A lake may
change from the eutrophic to mesotrophic zone as a result of an
altered phosphorus load and still not experience a significant
change in water quality.

     Figures 82, 83 and 84 can be used to evaluate the expected
changes in water quality resulting from various phosphorus loading
reduction scenarios.  In order to inject realism into the use
of this model, as well as the others developed in this section,
it will be assumed that the chlorophyll a concentration of the
hypothetical water body does not lie exactly on the line of
best fit.  Changes in the water quality parameters can then be
determined by moving the data point parallel to the line of
best fit  in the model.  Table 29 and Figure 82 indicate a chloro-

                                 302

-------
    10
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               20
                       log  Secchi  Depth--0.473 log [chlorophyll a] + 0.803
co
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           Z   10
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2
                                      1975 P-Load Minus 90 Percent
                                      Point Source P-Load and
                                      40 Percent Nonpoint Source
                                      P-Load
                                                     1975 P-Load Minus 90 Percent
                                                     Point Source P-Load
                                                                   1975 P-Lood Minus
                                                                   Detergent P-Lood
                                                 1975 P-Load
                          i  i
                                                             1   1 1
                                                                                        I  I  1
      0.5
                                                                   10
100
                    Figure  33.
                       MEAN  CHLOROPHYLL   a  CONCENTRATION (p. g/l)

                    Secchi Depth and Mean  Chlorophyll £ Relationship  as Applied  to  Hypo-
                    thetical Water Body  Under  Several Phosphorus  Loading  Scenarios.

-------
     100
               log Secchi Depth = -0.359log[(L(P)/Qs)/(l+./z/qs)] 40.925
 in
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LJ
      10
                                   !975P-Lood Minus
                                   90 Percent Point Source
                                   P-Lood ond 40 Percenl
                                   Nonpoint Source  P-Lood
                                                     !975P-Lood Minus
                                                     90 Percent Point Source
                                    1975 P-Lood Minus
                                    Detergent P-Lood
                                                             1975 P-Lood
                                           10
                                                                               100
                                       (mg/m )
            Figure 84.  Phosphorus  Loading Characteristics  and  Secchi
                        Depth  Relationship as Applied  to  Hypothetical
                        Water  Body  Under Several Phosphorus  Loading
                        Scenarios.
                                         305

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GO
O
CD
        z
        o

        LJ
        _J
        CL
        LJ
        O
O
CL
        LJ
        o:
              10
        o  E    1
             O.I
                                                  o
             log oreol hypolimnetic oxygen depletiontgOg/m /day)

                   = 0.467 log[(L(P)/qs)/(l+v'T5J")j-l.07
                   1975 P-Load minus90%
                   Point Source P-Load 8
                   40% Non-point Source
                   P-Load-
                                       »	1975 P-Load
      975 P-Load minus
     Detergent P-Load

 975 P-Load  minus
90% Point Source
P-Load
                                                  i  i  i  i i
                                                                            i  i i i
                             10
                                                          100
                                      1000
                Figure 85.
                                            (mg/m3)
                    Phosphorus Loading  Characteristics and Hypo!imnetic
                    Oxygen Depletion Relationships as Applied to
                    Hypothetical Water  Body  Under Several Phosphorus
                    Loading Scenarios

-------
phyll a concentration of 6.5 yg/1, based on the 1975 phosphorus
load. ~0n the basis of a detergent phosphate ban alone, there will
be a decrease of approximately 1.0 yg/1 in the chlorophyll a con-
centration of the hypothetical water body.  It should be noted that
changes of this magnitude are frequently within the experimental
error normally associated with chlorophyll a_ measurements on a
lake-wide basis.  On the other hand, a noticeable change will
be seen when the 90 percent  point source phosphorus removal
scenario is considered.  The mean chlorophyll a concentration
will drop from about 6.5 yg/1 to 3.9 yg/1, a decrease of approxi-
mately 40 percent.  A decrease of this magnitude is significant
and a noticeable increase in water quality, as reflected in
chlorophyll a content, would likely result in this hypothetical
water body.  Finally, if a 90 percent point source and a 40
percent non-point source phosphorus reduction are considered,
an additional decrease of 1,1 yg/1 chlorophyll a will be seen.
The chlorophyll a concentrations will have decreased from 6.5 yg/1
to 2.8 yg/1.  ThTs constitutes a 57 percent decrease in the
total chlorophyll a concentration in the water body compared
to a 66 percent decrease in the total phosphorus load.  This
low chlorophyll a level is typical of unproductive water bodies,
and would be  consistent with the oligotrophic status of the
hypothetical water body as indicated in the Vollenweider phos-
phorus loading diagram (Figure 81).

     The changes in Secchi depth which would be expected to
result from the various phosphorus load reductions are indicated
in Table 29 and Figures 83 and 84.  The predicted Secchi depth
based on the hypothetical 1975 phosphorus load will be approxi-
mately 2.6 m.  If a detergent phosphate ban is considered,
the Secchi depth will increase approximately 0.2 m.  This is
equivalent to an increase of about 8 percent resulting from a
35 percent reduction in the  point source loading.  As with the
chlorophyll a_ concentration, this amounts to an essentially
undetectable change in the Secchi depth on the basis of a deter-
gent phosphate ban alone in  the hypothetical water body.  The
change is more significant when the 90 percent reduction in
point source phosphorus loads is considered.  The Secchi depth
increase will be 0.8 m, a definitely discernible Secchi depth
increase of about 30 percent for the 50 percent decrease in
the total phosphorus load.  Finally, when both the 90 percent
point source and 40 percent  non-point source phosphorus load
reduction is considered, the Secchi depth increases from 2.6
to 3.9 m, an overall increase of 1.3 m.  This constitutes a
33 percent overall increase  in Secchi depth for a 66 percent
overall decrease in phosphorus load.
                                307

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     The hypolimnetic oxygen depletion rate changes to be
expected from the various phosphorus loading scenarios is
indicated in Figure 85.  The predicted 1975 areal hypolimnetic
oxygen depletion rate is  0.60 g C^/m^/day.  When the
detergent phosphate ban is considered, the rate decreases
approximately   0.1 g 02 for each m? of hypolimnetic area.
This is a 17 percent decrease for a 33 percent decrease in
the point source phosphorus loading.  The 90 percent point
source phosphorus loading reduction results are more signifi-
cant, with the hypolimnetic oxygen depletion rate dropping
to 0.37  g 02/m^/day.  This corresponds to a 38 percent
decrease in the oxygen depletion rate for a 50 percent
reduction in the phosphorus load.  When both the point and
non-point phosphorus load reductions are considered, the
hypolimnetic oxygen depletion rate decreases to 0.32 g
02/m2/day, an overall decrease of 47 percent for an overall
66 percent reduction in the phosphorus load to the hypothetical
water body.

     The improved water quality associated with 40 percent
control of phosphorus from diffuse sources will almost
certainly be less than that predicted in Table 29.  As a
result of the fact that many diffuse sources of phosphorus
such as urban and rural drainage and the atmosphere usually
have large parts of their phosphorus in a particulate form,
much of which is unavailable to support algal growth.

     Several points should be noted on the use of this
approach.  First, it is important to emphasize that the
magnitude of the changes discussed in the chlorophyll a_
or Secchi depth relationships refer to changes associated
with planktonic algal growth.  At present, there is no
information available for reliably predicting the effects of
a reduced phosphorus load on the growth of Cladophora and
other attached algae, as well as the growth of macrophytes
and floating macrophytes, such as water hyacinths and duckweed.
There is also no information available for reliably predicting
the effects of a phosphorus load reduction on water clarity
in the nearshore waters of a water body.  Further, water
bodies will not adjust immediately to an altered phosphorus
load.  Rather, it will require a period of time equal to three
                                 308

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                              Table  29.  SUMMARY OF PHOSPHORUS  LOADING  CHARACTERISTICS,
                                         CHLOROPHYLL a AND  SECCHI  DEPTH OF  HYPOTHETICAL
                                         WATER BODY UNDER SEVERAL  PHOSPHORUS  LOAD
                                         REDUCTION SCENARIOS
Phosphorus
Loading
Si tuationa
(L(P)/q )/(!+ Jz/(\ )
S Q S
( mg/m* )
Hypolimnetic
Chlorophyll a Secchi Depth Deoletion
t) f \ C /
((JR/t) vm; (gO^/m /lay)
CO
CD
ID
1975 Phosphorus
Loading

1975 Phosphorus
Loading Minus
Detergent Phos-
phate

1975 Phosphorus
Loading Minus
90% Point Source
Loadi ng

1975 Phosphorus
Loading Minus 90%
Point Source and
i|0% Non-Point
Source Loading
                                                      25 .9
                                                      20 .8
                                                      13 .0
                                                       8 .4
6 .5
                                                                            5 .5
                                                                            3.9
2.8
2 .6
                  2.8
                  3 .'I
                                                                                              3.9
                                                                                                         0.32
                 Phosphorus loadings were taken from Table 28.
                 As determined in Figure 83, based on phosphorus load characteristics  indicated  in  this
                 table.
                °As determined in Figure 8f, based on phosphorus load characteristics  indicated  in  this
                 table.

                 As determined in Figure 85,  based on phosphorus load characteristics  indicated  in  this
                 table.

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phosphorus residence times (Sonzogni et a1.,  1976) before a
new equilibrium condition will be established in the water body.
The models presented in Figures 22, 79, and  80, may be appli-
cable when a new equilibrium state is reached in a water body.
For example, the use of this approach predicts a chlorophyll a_
concentration in Lake Ontario of about 4.5 yg/1, based on its
1973 phosphorus load.  However, chlorophyll  a values reported by
Dobson (1975), the International Joint Commission (1976b) and
Vollenweider (1976a) are in the order of six to eight yg/1 for
the openwaters of Lake Ontario.  These higher values are possibly
due to a non-equilibrium condition of Lake Ontario resulting
from its reduced phosphorus load.  Lake Ontario has not yet had
sufficient time to respond to this reduced phosphorus load.  How-
ever, the use of these models in successfully predicting
chlorophyll a concentrations and Secchi depths in Lake Washington
following completion of its sewage diversion project, which was
discussed earlier in the section, lends considerable support to
these approaches in assessing the resulting  water quality in a
water body following a change in its phosphorus load.  When this
approach is applied to the Great Lakes, the  results obtained with
the use of Figures 22, 78, 79, and 80 are in general agreement with
the observations of Gilbertson et aJL. (1972), Vollenweider et al.
(1974) and Dobson (1975) concernTng the Great Lakes.

      In  the Great Lakes consideration has to be given to the
 fact that the nearshore waters of the lakes  often have elevated
 concentrations of nutrients compared to the  open water.  This
 situation arises from the strong longshore  currents which tend
 to be present in large water bodies and which inhibit mixing of
 nearshore with offshore water.  Under these  conditions, a differ-
 ent mean depth/hydraulic residence time relationship should be
 used in order to predict nutrient load-response relationships
 than would be applicable to the open waters  of the lakes.

      Results of computations such as those  presented above on
 nutrient load-response relationships provide water quality
 managers and the public with the information needed to evaluate
 the magnitude of water quality improvement  associated with a
 particular nutrient control strategy.  In order to develop a
 meaningful nutrient control program it is necessary to evaluate
 the costs associated with each approach. The cost of each
 nutrient control program and the degree of  water quality im-
 provement can be used to choose the most cost-effective control
                                310

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program.  Prior to the development of these relationships be-
tween phosphorus loading and water quality, as measured by
chlorophyll a concentrations, Secchi depth, and hypolimnetic
oxygen depletion, there was no readily available, and reliable,
method for predicting the expected improvement in water quality
resulting from a reduction in the phosphorus loading to a
water body by a certain degree.  Water quality managers can now
develop cost-effective eutrophication control programs in which
they can inform the public of the degree of improvement in water
quality expected to result from expenditure of funds by a certain
amount.  The taxpayers can then decide how much they are willing
to pay for improved water quality.
APPLICATION OF  RESULTS TO  IMPLEMENTATION  OF  SECTION  314-A
OF  PUBLIC  LAW 92-500


      Section 314-A of PL 92-500 requires  that  each state clas-
sify  its lakes  and impoundments with respect to their  degree
of  fertility.   Furthermore, the states must  develop  a  nutrient
control program to minimize fertility  in  those water bodies
found to be excessively fertile.  The  results  of this  investi-
gation of  the US OECD eutrophication study provide the  states
and the federal government both with a basis by which  this
type  of classification can be made , and  with the ability to
assess the improvement in water quality that is likely  to re-
sult  from  a nutrient control effort of a  certain magnitude.
From  a water quality management point  of  view, the expected
improvement from a nutrient control program  can be weighed
against the cost of achieving the nutrient control, and a
decision can be made as to whether the control effort will
result in  a sufficient improvement in water  quality  to  justify
the expense of  the program.  It is important to look on the
results of this study as a guide for implementation  of  public
policy in  the area of excessive eutrophication of natural
waters.  While  Vollenweider, and others who  have modified
his approach, have been able to formulate nutrient load-
eutrophication  response relationships with a relatively simple
methodology, involving normalizing water  bodies based on
their mean depth and hydraulic residence  time, there are
many other factors which can influence the nutrient  load-
algal response  relationship in natural waters.

     Examination of the various plots presented in this report
show there is considerable scatter in the data.  Part of this
data scatter is due to differences in measurement techniques .
Another part is due to the inherent variabilitv of lakes and
impoundments.   With respect to measurement,  every point on any
of  the nutrient load-response diagrams usually has considerable
                                311

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variance in both the x and y directions.  One of the most diffi-
cult parameters to estimate for many water bodies is the hy-
draulic residence time.  This factor is continuously changing.
A series of wet years could markedly affect the results com-
pared to more normal or dry conditions.  For example, an im-
poundment in north central Texas shows a hydraulic residence time
from 0.3 years to 22 years, with a mean of about 4 years, depend-
ent upon wet and dry climatic cycles .   In addition to variable
climatic patterns, another factor to be considered is that of
short-circuiting of the inflow and outflow waters, such that
the inflowing nutrients do not interact with the total water
body.  This may be an especially significant problem for large,
deep impoundments.  Under these conditions, a modification of
the hydraulic residence time term should be made to more properly
reflect the actual behavior of the nutrients in the impoundment.
This modification should reflect the fact that some of the
nutrients that enter a water body may  leave it by way of outflow
before they have had the opportunity to interact with the phyto-
plankton.

     The variance about the vertical displacement on the diagrams
in this report, for a phosphorus load, chlorophyll, Secchi depth
or hypolimnetic oxygen depletion response, is likely to be very
large under certain circumstances.  The data presented in this
report are often based on a single year's measurements.  Lakes
and impoundments respond to nutrients  not only for the year in
which the nutrients are added, but for previous years' nutrient
inputs as well.  Each water body would have an individual response
in this respect.  Further work, which  will not be reported in this
report , is being done by these authors to estimate the magnitude
of the associated variance that is likely to be encountered with
measurements of the various load-response relationships.  From the
work done thus far, it is important that no regulatory agency re-
quire implementation of a control program because a water body's
phosphorus load plots just above the permissible or excessive
line on the diagrams presented in this report.  Similarly, no
regulatory agency, or other group, should assume that because a
lake plots just below the excessive or permissible line, that
this lake will not have water quality  problems due to excessive
fertilization.

      Factors  such  as  color,  turbidity,  morphological  shape  of
the  water  body basin,  rainfall  runoff patterns,  characteristics
of the  watershed,  etc.,  all  would  have  an influence  on the
nutrient load-response  relationships  in natural  waters,  and  all
contribute  to the  scatter  of points in  the  various nutrient  load-
response evaluations  made  in this  study.   One  of the  areas  of
research that needs  considerable  additional  attention, in attempt-
ing  to  reduce the  scatter  in the  data,  is that of'available
nutrients.   In general,  the  various diagrams  presented in this

                                312

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report are based on total phosphorus.   It is well known that
only part of this total phosphorus is  available.   As a guide-
line, Cowen and Lee (1976b)have concluded that the best approach
for estimating the available phosphorus load to a water body
is that it is equal to the soluble orthophosphate load plus 20 per-
cent of the difference between the total phosphorus and soluble
orthophosphate load.  This difference  between the total and soluble
orthophosphate is made up of inorganic and organic forms which
are generally particulate.  These results indicate that for
those water bodies in which the primary source of nutrients is
from agricultural or land runoff, a substantial part of the
phosphorus may not contribute to the algal-available load.

     Another factor to consider concerns the amounts of available
phosphorus that reach a water body when the origin of the phos-
phorus load is a considerable distance from the water body.
For example, in the US-Canadian Great  Lakes, some controversy
has developed concerning the significance of domestic wastewater
discharges many miles from the lake in influencing excessive
fertilization problems in the Great Lakes.  If the wastewaters
enter a lake somewhere between their origin and the Great Lakes,
most of the nutrients would be retained in the intermediate lake,
since many water bodies trap from 60 to 90 percent of the
phosphorus that enters them by incorporation of the phosphorus
into the sediments.  Further, as the available phosphorus added
to a stream some distance from the lakes mixes with the erosional
materials, and/or is utilized in various biological processes,
it is becoming less and less available for stimulation of algal
growths.  It is likely that available  nutrients discharged to
rivers which are considerable distances from the lake of interest
will have much less influence on stimulating extensive fertiliza-
tion problems than would the same nutrients discharged directly
to the water body.

     Special consideration in assessing nutrient sources should
be given to septic tank wastewater disposal systems since a large
part of the US population utilizes this form of wastewater dis-
posal.  A comprehensive review on the  significance of septic
wastewater disposal systems as a source of phosphorus has recently
been completed by Jones and Lee (1977).  They concluded that,
with few exceptions, the phosphorus present in septic tank domestic
wastewater disposal systems will have  little influence on stimulat-
ing excessive fertilization problems in natural waters.  Guidance
is provided by Jones and Lee in evaluating, on a case by case
basis, whether in a localized area excessive fertilization prob-
lems are caused by  septic tank systems.

     While this report has focused primarily on the application
of the Vollenweider loading approach to assessing water quality
in which the water  quality problems are related to excessive
fertilization for whole bodies of water,  it is applicable to
parts of a water body as well.  A number  of the lakes and impound-
ments investigated  in this report were subdivided into various

                               313

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sections or arms.  The results appear to indicate that this approach
is appropriate.  Further, for large water bodies such as the Great
Lakes, the approach described in this report should be applicable
to bays and nearshore waters as well.  In the case of the Great
Lakes, it is important to be able to estimate the exchange of
water between the nearshore and offshore zones, or between the
openwaters of the lakes and their bays.  It is important to em-
phasize once again that the Vollenweider loading approach is
directly applicable to the management of water quality problems
associated with excessive fertility, as manifested in phytoplank-
ton growth which cause an impairment of recreational use of water.
Further research is likely to produce the information needed to
develop modifications of the Vollenweider loading relationship
for other water quality problems such as excessive growth of
macrophytes, attached algae, dissolved oxygen depletion in the
hypolimnion and impairment of water supplies for domestic and
industrial use.  Further work, which will be reported by these
authors in subsequent reports, is being done along these lines
in order to define conditions for which the Vollenweider loading
approach is not applicable.  It is already apparent from this
study that the Vollenweider approach must be modified for those
water bodies which show very short hydraulic residence times
because the nutrients entering into the water body could pass
through it before interacting with the phytoplankton and thus
would not produce an algal crop in the water body proportional to
its nutrient loading.  In these cases, the Vollenweider loading
approach, in its present form, would be inappropriate for
assessing the eutrophication status of the water body.

AN APPROACH FOR THE USE OF THE VOLLENWEIDER NUTRIENT LOAD-WATER
QUALITY PROGRAM

     The procedures that should be utilized in applying the Vollen-
weider loading relationship for the development of a water quality
management program designed to Improve water quality or minimize
future deterioration  are presented below.

     1.  Determine the limiting nutrient.  Since the Vollenweider
loading relationship was derived for phosphorus, the first step
in its application would be to determine whether phosphorus or
nitrogen is the limiting nutrient in the water body.  This assumes
that all other factors affecting algal growth (i.e., light and
temperature) do not limit the maximum algal biomass that will
develop and that it is the concentration of the limiting nutrient ,
relative to the stochiometric needs of the algae, which controls
or limits the deterioration of water quality.

     The limiting nutrient can usually be determined by several
techniques, including N:P ratios, bioassay studies or simple ob-
servation of the available nutrient concentration dynamics over
the seasonal and/or annual cycle (Lee, 1973).  The use of the
growing season inorganic nitrogen: soluble orthophosphate mass
ratio  (expressed as N:P) in a water body was discussed in an

                                314

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earlier section of this report (see Tables 9 and 10).  Bioassay
techniques can also be used to determine the limiting nutrient in
a water body.  The algal assay procedure provides a standardized
test for identifying algal-growth-limiting nutrients in water
bodies, for determining the biological availability of algal
growth-limiting nutrients, and for quantifying algal responses
to changes in concentrations of the nutrients (Sridharan and Lee,
1977).   An estimate of the limiting nutrient can be obtained by
observing the dynamics of the available nutrients during the grow-
ing season.  If one of the algal-available nutrient forms becomes
depleted in a water body at the same time that the other is still
present in large quantities, it is usually reasonable to assume
that the depleted nutrient may be the algal-growth-limiting
nutrient.

     If it is determined that nitrogen, rather than phosphorus,
is the aquatic plant growth-limiting nutrient, then two options
are available.  One can either attempt to control the nitrogen
loading, or else reduce the phosphorus loadings to such an extent
that phosphorus becomes the limiting nutrient.  The latter course
of action is almost always preferred, for reasons mentioned in
earlier sections of this report (Vollenweider, 1968; 1975a; Lee,
1971;  1973; Vallentyne, 1974;  Golterman,  1976).   It does not
matter that nitrogen initially controls the algal growth in a
water body, but rather that phosphorus can be made limiting in
the water body.  To determine if it is possible to change a water
body from nitrogen-limitation to phosphorus-limitation, one must
be able to assess the potential benefit that might be derived
from a reduction of phosphorus in a water body.  Sridharan and
Lee (1977) have recently developed a technique for making such an
assessment.  This procedure is based on studying the response of
algae to alum-treated lake water  and has worked well for evalu-
ation of the potential benefit to be derived from a phosphorus
reduction in Lake Ontario.  Based on the results of these types
of analyses, one can determine the limiting nutrient in a water
body,  and make an evaluation of the potential benefits, in terms
of algal growth responses, to be derived from a decrease in the
phosphorus content of a water body.

     2.  Determine the available nutrient sources and significance
of each source.  This step consists of quantifying the nutrient
loadings to a water body.  It is first necessary to identify all
the sources of nutrient inputs, both point and non-point sources.
Sonzogni and Lee (1974) have presented an extensive examination
of the estimated nutrient loadings to Lake Mendota in 1972.  The
approach used by Sonzogni and Lee is an example of how one may
assess the nutrient sources to a water body.  They examined the
nutrient inputs from waste water discharges, urban, rural and
forest runoff, groundwater seepage, baseflow, nitrogen fixation
and from the atmosphere directly onto the lake surface.  They
then determined the total nutrient loadings from these sources.
This same approach can be used to assess the nutrient sources
for most water bodies.

                                 315

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     Once the sources are identified, one may then quantify the
nutrient inputs for a water body.  The loadings may be directly
measured or determined by indirect methods.  If it is measured
directly, the sampling program should be sufficient to allow one
to determine the variability from a particular source.  For ex-
ample, the amount of phosphorus from a sewage treatment plant can
be determined by measuring the phosphorus concentration in the ef-
fluent and multiplying this concentration by the flow.  The result
will be the mass of phosphorus loading from this source.   However,
the phosphorus concentrations in sewage treatment plants  can vary
widely over a daily, weekly and monthly cycle.  This variability
must be determined so that accurate loads from this major nutri-
ent source can be computed.  Another case of variability  involves
measurement of land runoff.  According to Kluesner and Lee (1974),
the phosphorus concentration in urban runoff after a storm varies
widely, usually reaching a peak which is not coincident with the
peak runoff flow.  Thus, the concentrations and flows may have to
be measured frequently during a storm if it is desirable  to get
very accurate loading estimates during this period.

     An alternative to direct measurement is to use watershed land
use nutrient export coefficients.  This method was used in this
report and is described in detail in an earlier section.   This
method is based on the fact that a given land use activity with-
in a watershed will produce a relatively constant nutrient ex-
port over an annual cycle (i.e., an acre of corn field or urban
area will produce about the same annual export of phosphorus and
nitrogen).  Thus, loadings to a given water body can be determined
on the basis of land use type in the water body's watershed and
use of the appropriate nutrient export coefficient.  A number of
studies concerning export coefficients for various land uses have
recently been completed (Vollenweider, 1968; Sonzogni and Lee,
1974; US EPA, 1974c; Vollenweider and Dillon, 1974; Uttormark
et a_l. 1974; Dillon and Kirchner, 1975; Dillon and Rigler, 1975).
One can determine phosphorus and nitrogen loadings from sewage
treatment plants in a similar manner.  Several studies have been
conducted to estimate per capita nutrient concentrations  in
domestic wastewaters (Vollenweider, 1968; Sonzogni and Lee, 1974;
Dillon and Rigler, 1975).  One may use these reported values or
experimentally determine the per capita loadings by direct measure-
ments.  The reader is referred to these various studies for ap-
propriate nutrient export coefficients on per capita inputs.  If
it is felt that a given export coefficient is not accurate for a
given land use, an alternative is to directly measure the nutrient
runoff from a land use type in the watershed and formulate one's
own coefficients.

     Using these methods, the loadings of total phosphorus and
nitrogen, as well as algal-available phosphorus and nitrogen to
a water body can be computed.  One can also then evaluate the
relative significance of each source if it is necessary to choose
between controlling the input from several sources.  The  nutrient

                                 316

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loadings from domestic sewage treatment plants is usually one of
the most significant sources for most water bodies.   One should al-
so evaluate the loadings of available nutrients versus the loadings
of total phosphorus and nitrogen, since the available nutrients are
the ones assimilated by algal populations in water bodies.  As
mentioned earlier (Cowen and Lee, 1976b), the best estimate of the
loading of available phosphorus is that it is equal to the sum of
the available phosphorus loading plus 20 percent of the difference
between the total phosphorus and available phosphorus loading.

     3.   Assess the nutrient load-eutrophication response relation-
ships .  When an estimate of the available phosphorus loading is
available, the next step is to assess the relationship between the
loading and the eutrophication responses of a water body.  This
assessment assumes that the computed phosphorus loading is accurate.
The accuracy of the phosphorus loading estimate, whether measured
or computed using nutrient export coefficients , can be checked us-
ing the  relationship developed by Vollenweider between the ratio of
the mean total phosphorus concentration to the influent phosphorus
concentration and the hydraulic residence time (see Equation 26
and Figure 14).  This approach was presented in an earlier section
of this  report.  The phosphorus and nitrogen loading estimates, if
they were directly measured, could also be checked using appropriate
watershed nutrient export coefficients.

     After the reasonableness of the loading estimates, particular-
ly phosphorus, has been determined, the relationships presented
in earlier sections of this report can be used to assess the rela-
tive degree of oligotrophy or eutrophy of a water body.  The
critical phosphorus loading levels can be determined for a water
body.  Also, the expected enhancement or deterioration of water
quality  following a phosphorus loading reduction or increase,
respectively, can be evaluated.  This can be done in a manner
completely analagous to that presented by Lee (1976) concerning
the expected effects of a phosphate detergent ban in the State of
Michigan on v.'ater quality in the Great Lakes.

     The relative trophic condition of the water body can be
determined using the Vollenweider phosphorus loading and mean
depth/hydraulic residence time relationship (Figure  19).  To
evaluate the water quality, the relationship between phosphorus load-
ing and mean epilimnetic chlorophyll a concentration in a water body
can be determined with the use of Figure 22. One can next determine
the expected water clarity for a given phosphorus load with the
use of Figure 79.  If a large quantity of data is available for a
water body concerning its chlorophyll a concentrations and cor-
responding Secchi depths , one can construct a Secchi depth and
chlorophyll a concentration diagram specific for that water body.
This can be transformed into a phosphorus load characteristics
and Secchi depth diagram in the same manner as was done with
Figure 79.  Otherwise, Figure 79 can be used in its  present
form.

                                 317

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     One could also use these relationships to evaluate the
expected changes in water quality in a water body in future years
as a function of future changes in phosphorus loads.  Figures 19,
22,79 and 80 can be used in the same manner as indicated above for
evaluating expected future phosphorus loads.  Particularly,
Figure 19 can indicate the expected relative changes in trophic
condition resulting from an altered phosphorus load.  Figures 22, 79
and 80 can be used to predict the expected changes in mean
chlorophyll a concentrations, Secchi depth and hypolimnetic oxygen
depletion for an altered phosphorus load.

     4.   Evaluate cost-benefit analysis of eutrophication control
program.  Most eutrophication control programs are based on reduc-
tion of phosphorus loads to a water body.   As indicated above, the
expected water quality changes can be evaluated for a given phos-
phorus load reduction.  The final question then involves the cost-
benefit of any given eutrophication control program.  Previously,
eutrophication control programs based on phosphorus load reduction
were largely subjective in nature.  The use of the above-mentioned
relationships provides individuals concerned with water quality
management with a quantitative tool to evaluate expected changes
in water quality resulting from eutrophication control programs
based on reduction of phosphorus inputs.  The final question to
be answered concerns evaluation of the relative monetary worth of
such a eutrophication control program.  Do the results of a phos-
phorus removal or sewage diversion program, for example, justify
the funds expended for the project?  In short, is the final ex-
pected product worth the money?

     This final question brings social, economic and political
considerations into the overall picture.  Lee (1971; 1973) and
Vollenweider and Dillon (1974) have determined that widespread
use of phosphorus removal programs is economically feasible.
Lee (1971; 1973) has determined that phosphorus removal from
domestic wastewaters is possible for a cost of about one cent
per person per day.  It is then up to those individuals con-
cerned with water quality management to determine if it is worth
one cent per person per day to produce a change in water quality
as predicted with the use of Figures 22, 79 and 80 For example, if
it is shown that the phosphorus loading to a water body can be
reduced by 60 percent by initiating advanced waste treatment for
phosphorus removal from domestic waste waters, and that such a
reduction will lower the mean chlorophyll a concentration from
10 yg/1 to 5 ug/1 and raise the Secchi depth from 1 meter to
2 meters, is the cost of building and operating the plant justi-
fied by the expected improvements in water quality?  This will
have to be evaluated on an economic and political level since
such programs are usually ultimately funded by the taxpayers.
The important point to be made is that now the individuals who
must pay for eutrophication control programs can be shown in ad-
vance of the initiation of such programs what they will get in
terms of improved water quality for their money.  They can then
decide, by whatever means they choose, whether the expected im-
provements are worth the expected costs to them.

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     It is expected by these authors that additional quantitative
tools for evaluating predicted changes in water quality will be
developed in the future, providing further methodologies for
making water quality management cost-benefit analysis decisions.
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                           SECTION XII

                   TROPHIC STATUS INDEX STUDY


     The US OECD data base offers an opportunity to examine the
comparability and to some degree the reliability of several recently-
proposed water body trophic status indices.  This section of this
report is devoted to a review of these trophic status index schemes
and an analysis of the results of the trophic classifications of
the US OECD water bodies.

GENERAL CONSIDERATIONS

     Lakes and other surface waters are characteristically divided
into two general categories, oligotrophic and eutrophic.  Further,
it is generally agreed that mesotrophic describes water bodies in
a transition state between oligotrophic and eutrophic (Fruh et al.
1966; Vollenweider, 1968; Lee, 1971; Vallentyne, 1974).  However^"
the exact meaning of these three terms is still debated among lim-
nologists because of a lack of understanding concerning details of
the eutrophication process, other than on a gross level, and its
effects on the aquatic environment.

     Weber (1907, as cited in Hutchinson, 1969), was the first to
introduce the terms "eutrophic" and "oligotrophic."  He used these
terms to describe the general nutrient conditions of soils in Ger-
man bogs.  The succession of Weber's scheme ran from eutrophic to
oligotrophic as a submerged bog was built up to a raised bog.  The
submerged bog was characterized as eutrophic or well-nourished,
while the raised bog was characterized as oligotrophic.  Naumann
(1919, as cited in Hutchinson, 1969), introduced these terms into
limnology.  Naumann used the term "eutrophic formation" to describe
a phytoplankton assemblage in nutrient-rich waters.  Naumann (1931,
as cited in Stewart and Rohlich, 1967) later refined his definition
of eutrophication as "an increase of the nutritional standards (of
a body of water), especially with respect to nitrogen and phosphorus.'

     As originally defined, eutrophic and oligotrophic referred to
water types (i.e., quality of water).  However, the term has general-
ly come to refer to general lake types, including the physical,
chemical and biological characteristics of the water body and its
drainage basin (Brezonik et a1., 1969).  The difficulty in defining
the terms oligotrophic an3~~eut"rophic is related to the fact that
these terms are used in different ways by different investigators.
Some use these terms to refer to aquatic plant nutrient flux, others
use them to describe plant and animal production, while even others

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            Table 30.   GENERAL CHARACTERISTICS FREQUENTLY
                       USED TO CLASSIFY WATER BODIES
                                        General Characteristic
         Parameter
    Oligotrophic
     Eutrophic
Aquatic plant production low
Algal blooms rare
Algal species variety many
Characteristic algal groups 	

high
many
variable to few
blue-green
Anabena
Aphanizomenon
Microcystis
Oscillatoria
                                                       rubescens
Littoral zone aquatic plant
  growth

Aquatic animal production

Characteristic zooplankton
Characteristic bottom fauna

Characteristic fish
Oxygen in the hypolimnion
Depth

Water quality for most
  domestic and industrial
  use
Total salts or conductance

Number of plant and an-imal
  species
sparce
low
abundant
high
Bosmina obturirostris B_. longirostris
B_. coregoni           D_. cucullata
Diaptomus gracilus
Tanytarsus

deep-dwelling, cold
water fishes such as
trout, salmon and
cisco
present
tend to be deeper
good
usually lower

many
Chironomids

surface-dwelling,
warm water fish
such as pike,
perch and bass;
also bottom dwell-
ing fish such as
catfish
absent
tend to be
 shallower
poor

sometimes higher

few
Taken from Fruh et_ al. (1966); Lee (1971); Vallentyne (1974)
                              321

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use them to describe the process of excessive discharge of aquatic
plant nutrients to a water body that results in water quality dete-
riotation (Lee, 1971).

     Even though there is general agreement concerning a oligotrophic-
eutrophic succession scheme, the problem of the trophic status or
classification of a water body at a given point in time remains to
be considered.  This illustrates a basic problem in lake classifica-
tion, namely that the exact classification of a water body is usual-
ly related to its intended use.  A water supply reservoir manager
would likely have a much more stringent definition of eutrophic
than would a fisherman who was interested in fish production.  They
would desire opposite ends of the trophic spectrum; hence, their
views of oligotrophy versus eutrophy would also likely be different.

     However, there are some relatively widely-accepted general
characteristics used to characterize lakes.  Table 30 summarizes
the commonly accepted characteristics of oligotrophic and eutro-
phic lakes.  The reader is also referred to recent reviews of the
eutrophication process and its manifestations (Sawyer, 1966; Ameri-
can Water Works Association, 1966; Fruh et_ al., 1966; Stewart and
Rohlich, 1967; Vollenweider, 1968; Brezonik et_ aJL. , 1969; Federal
Water Quality Administration, 1969; National Academy of Sciences,
1969; Lee, 1971; Likens, 1972a; US EPA, 1973a; and Vallentyne,
1974) .

     Examination of Table 30 shows that oligotrophic lakes tend to
have a low nutrient flux relative to their volume of water.  They
contain small amounts of organisms, but many different species of
both aquatic plants and animals.  In general, oligotrophic lakes
are deep, with average depths of 15 meters or greater and maximum
depths frequently greater than 25 meters (Vallentyne, 1974).  How-
ever, this feature is highly variable.  Further, as oligotrophic
lakes fill, due to sediment'deposition over time, they will tend to
become eutrophic (Lee, 1971).  Oligotrophic lakes usually have high
dissolved oxygen concentrations in the hypolimnion during all
periods of the year, including the growing season.  This oxygen-
containing, cool hypolimnetic region is the home of the trout,
walleye, cisco and other cold water prized game fish sought by
fishermen.  The water quality is generally good the year round in
oligotrophic lakes.  In general, oligotrophic lakes can be charac-
terized as deep, transparent water bodies with a low nutrient flux
relative to their ability to assimilate the nutrients.

     By contrast, eutrophic water bodies have a high nutrient flux
relative to their water volume.  As a result, they are highly pro-
ductive water bodies with large amounts of aquatic life, but of
somewhat fewer species than oligotrophic lakes.  They are highly
productive at all trophic levels and frequently experience algal
blooms, especially during the growing season.  Characteristic
algae include the nuisance blue-green species associated with
deteriorated water quality.  The same is true for all other aquatic
life species.  The fish are usually the "coarser" species not

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generally sought by most fishermen (though this may vary from loca-
tion to location).   They are generally shallow, often with exten-
sive littoral areas with abundant plant growths.   Mats of macro-
phytes and attached algae may carpet the littoral zone, depending
on competition between planktonic and attached plants and on the
normally higher turbidity waters in eutrophic water bodies.  Eu-
trophic lakes deep enough to develop a thermocline usually show a
partial or complete dissolved oxygen depletion in the hypolimnion.
The extent of oxygen depletion will depend on the amounts of
aquatic plants that develop in the surface waters, and may become
super-saturated with oxygen due to the increased photosynthetic
activity at the surface.  The surface water typically becomes
turbid in the summer as a result of algal growth, to the extent
that, with few exceptions, the amount of aquatic plants produced
will be restricted to the surface waters.  Such turbidity restricts
light penetration to the epilimnetic waters, with the result that
the Secchi depth is usually three meters or less (in contrast to
the 10+ meters of some oligotrophic waters).

     There also appears to be a correlation between the total dis-
solved salt content or conductivity and the increased primary pro-
ductivity, presumably because the higher salt content is related to
a high aquatic plant nutrient flux.  In general,  the overall water
quality is poor as a result of the increased nutrient flux and re-
sultant increased growth at all trophic levels.

     In spite of these generally-accepted characteristics of pro-
ductive versus nonproductive, the problem of the absolute classifi-
cation of the trophic conditions of water bodies is still unsettled
Individuals tend to subjectively classify water bodies on the basis
of some of the common, though arbitrary, trophic state indicators
listed in Table 30 (i.e., nutrient concentrations, Secchi depth,
hypolimnetic oxygen depletion, chlorophyll concentrations, etc.).
A strict agreement is missing on what standards or values of these
and other parameters constitutes a given trophic state.  This
interpretation still varies widely among investigators.

REQUIREMENTS FOR A TROPHIC STATUS CLASSIFICATION INDEX

     The traditional water body classification scheme of oligotro-
phic, mesotrophic and eutrophic is inadequate for descriptive
purposes other than in a very broad sense (Shapiro, 1975b).  As
a result, there has been a development of several trophic clas-
sification systems in an attempt to classify lakes on a quantita-
tive basis.  A variety of characteristics of water bodies have
been used as a basis for various classification schemes and many
of the schemes use markedly different approaches.

     An adequate trophic index scale or scheme is particularly
needed in view of the mandates of Public Law 92-500.  Section
314-A of this law requires that each state classify its lakes
according to their trophic condition.  Further, eutrophication
control measures must be initiated by the states in water bodies

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deemed to be excessively fertile.   The question then arises as to
which classification or index scheme to use.   The array of trophic
indices used in the past is both wide and diverse.  These range
from determining recreational potential, management purposes and
scientific studies.  The indices may be descriptive or analytical,
subjective or objective, simple or complex, relative or absolute,
biological, physical and/or chemical, etc. (Shapiro, 1975b).

     There is need for a numerical trophic state index which will
permit a more appropriate assignment of the trophic condition of a
water body than the previous broad descriptions of oligotrophic,
mesotrophic and eutrophic.   The index scheme should be simple,
based on practical parameters whose values can be determined rela-
tively easily and which do not require sophisticated methods of
statistical analysis.  More complicated trophic classification
indices could be developed, but would likely have limited use.

     An example of a more complex scheme is a trophic state index
based on the simultaneous use of multiple factors developed by
Shannon and Brezonik (1972).  They based their multivariate ap-
proach on seven trophic state indicators, including primary pro-
ductivity, chlorophyll a, total phosphorus, total organic nitrogen,
Secchi depth, specific conductivity and Pearsall's cation ratio
(i.e., (Na+K)/(Ca+Mg)).   Shannon and Brezonik applied their clas-
sification system to 55 lakes in Florida and found a good correla-
tion between the trophic status index values obtained using their
approach and the traditional trophic classification of these water
bodies.  This index has problems based on the amount of data needed
for its use.  For many water bodies, it is not always possible to
obtain all the data needed for classification.  Shapiro has
criticized the Shannon and Brezonik approach since it tends to mis-
classify water bodies.  According to Shapiro (1975b), when Shannon
and Brezonik (1972) applied their trophic index system to Lake Alice,
one of the 55 Florida lakes in their study, it had a TSI value of
10.7.  This places it in a hypereutrophic category, relative to the
other lakes in their study.  However, Lake Alice has a low primary
productivity and chlorophyll concentration, inconsistent with a
hypereutrophic water body.

     Three trophic index schemes which show varying degrees of
promise have recently been developed.  These include the trophic
classifications of the US EPA (1974d), Carlson (1974) and Piwoni
and Lee (1975).  In addition, a trophic index system based on
Vollenweider's phosphorus loading diagram (Figure 19) has been
developed as part of this report.   These classification schemes
are discussed below.

CURRENT TROPHIC STATUS CLASSIFICATION INDICES

US EPA Trophic Status Index System

     The US EPA (1974d)  Trophic Index System was developed as part
of the National Eutrophication Survey.  This system is a variation
of a ranking method used by Lueschow et al. (1970) for 12 lakes in
Wisconsin.  Lueschow et al. used several unweighted characteristics

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of a water body which each reflect, in one way or another, its
trophic condition.  From these parameters Lueschow et al. derived
a composite rating which was the sum of the numerical values of
position for each of the parameters used in the index.  The para-
meters used were dissolved oxygen (DO) 1 meter above bottom,
organic nitrogen, total inorganic nitrogen, Secchi depth and net
plankton.  The water body with the lowest composite value was
judged the most oligotrophic and the highest composite value
lake was judged the most eutrophic.

     The US EPA based their initial index system on 200+ lakes
surveyed in 1972 (US EPA, 1974d).  Ultimately 812 lakes will form
the data base.  However, rather than using the positional ranking
used by Lueschow et al., the US EPA adopted a percentile ranking
procedure.  For each of the unweighted characteristics used, the
percentage of each of the 200+ lakes exceeding a given lake in
that parameter (i.e., chlorophyll a concentration, for example)
was determined.  The final ranking or index value was simply the
sum of the percentile ranks for each of the parameters used.  The
six parameters used in the US EPA Trophic Index System are sum-
marized in Table 31.  The values for the Secchi depth and minimum
DO were subtracted from a fixed value (500 inches and 15 mg/1,
respectively) so that all parameters would contribute a positive
number to the ranking system.  Using this system, a single index
number was produced for each lake, so that a large number of lakes
could be ranked in relative order from most oligotrophic to most
eutrophic.  However, this system does have several problems.
This system sums the rankings for each parameter of a given water
body, and thus loses information concerning specific water body
characteristics.  Furthermore, according to the US EPA (1974d),
water bodies with very short hydraulic residence times and those
with extensive littoral zones and excessive macrophyte production
do not seem to fit the scheme.  In the first case, the high
flushing rates can cause relatively low mean nutrient concentra-
tions in spite of high nutrient loadings.  In the latter cases,
the macrophytes may effectively compete with the algae for avail-
able nutrients, producing low nutrient and chlorophyll a levels
and relatively high Secchi depths in spite of a highly eutrophic
condition.

     Table 31.  US EPA TROPHIC STATE INDEX PARAMETERS
     1.  Median Total Phosphorus Concentration (mg/1).
     2.  Median Inorganic Nitrogen Concentration (mg/1).

     3.  500 - Mean Secchi Depth (inches).

     4.  Mean Chlorophyll a (yg/1).

     5.  15 - Minimum Dissolved Oxygen Concentration  (mg/1)
     6.  Median Dissolved Phosphorus Concentration (mg/1).


     Taken from US EPA (1974d).
                                325

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Carlson Trophic Status Index System

     Carlson's (1974) Index System is based upon Secchi depth as
a means of characterizing algal biomass.  As mentioned earlier,
this parameter, in the absence of turbidity and colored materials
in water, is a direct measure of planktonic-algal-manifested
eutrophication processes in natural waters.  Its range of values
can easily be transformed into a convenient scale.  Further, by
using empirically-derived relationships between Secchi depth and
both total phosphorus and chlorophyll a concentrations, Carlson
has derived equations to estimate the same index value from these
two parameters as well as from Secchi depth.

     Carlson's Trophic Index is basically a linear transformation
of Secchi depth,  such that each major unit in his scale has half
the value of the next lowest unit.  Conversely, for total phos-
phorus and chlorophyll a each major unit in his scale has larger
values for the next higher unit.  The computational form of the
equations for his trophic scheme is as follows:

          TSI(SD) = 10(6-log2SD),                           (43)


          TSI(Tp) = 10(6-log265 ip), and                    (44)


          TSI(Chlor) = 10<6-log27.7 	L_)              (45)
                                    Chlor0'68
     where SD = Secchi depth (m) ,

          TP = Total phosphorus concentration (yg/1),
     and Chlor = Chlorophyll a concentration (yg/l).
Calculation of the indices is facilitated by using these three
equations:

          TSI(SD) = 10(6 - In-f^'                          (46)
                              65

          TSI,mT,N = 10(6 - JlL-S , and                      (47)
                       ,n,c   2.04-0.68 In Chlor a
             (Chlor) = 10(6	-TF-2	          (48)

The trophic scale and associated parameter values are presented in
Table 32.

     According to Carlson (1974),  this index system has the
advantages of easily obtained data,  simplicity of form (i.e.,
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         Table 32.  THE CARLSON TROPHIC STATE INDEX
                   AND ITS ASSOCIATED PARAMETERS

TSI
0
10
20
30
40
50
60
70
80
90
100
Secchi
Depth
(m)
64
32
16
8
4
2
1
0.5
0.25
0.12
0. 062
Surface
Phosphorus
(mg/m3 )
1
2
4
8
16
32
65
140
260
519
1032
Surface
Chlorophyll
(mg/m3 )
0.
0.
0.
0.
2.
6.
20
56
154
427
1183
04
12
34
94
6
4





Taken from Carlson (1974).
                                327

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trophic condition reported as a single number), objectivity,
absolute TSI values, valid relationships, retrieval of data from
the index (i.e., information is not lost, as in the US EPA and
Lueschow index systems) and can be intuitively grasped by the
layman in much the same manner as the Richter earthquake scale.

Piwoni and Lee Trophic Status Index System

     A trophic index scale has been proposed by Piwoni and Lee
(1975).  This index system was derived in a manner analogous
to that of Lueschow et al.  (1970), except it contains modified
and additional parameters.   The trophic state parameters are
summarized in Table 33.  The total inorganic nitrogen parameters
were later dropped because the Wisconsin water bodies from which
the index system was derived, and on which it was first tested,
were not nitrogen-limited with respect to aquatic plan nutrient
requirements.

     The sum of the rankings of the water bodies, after examina-
tion of the 10 trophic index parameters, was used to classify a
water body.   The water body with the lowest overall ranking number
was judged the most oligotrophic of the water bodies being con-
sidered.  Like the US EPA (I97<4d) and the Lueschow et_ al. (1970)
trophic index systems, the Piwoni and Lee (1975) system is a
relative trophic ranking system with the water body of the high-
est water quality receiving the lowest trophic index number.

     The Piwoni and Lee system has a significant advantage over
the Lueschow et al.  and US EPA systems in that it attempts to
eliminate from the classification those parameters (characteris-
tics) which may not properly characterize a water body's trophic
state.  For example, for water bodies in which the chemical
nutrient determining overall algal biomass is phosphorus, (i.e.,
phosphorus-limited lakes) a classification system that utilizes
inorganic nitrogen concentrations would incorporate extraneous
information which would not be directly related to the overall
water quality of the water body as it relates to excessive
fertiliation.

     One of the primary values of the multiparameter trophic
state index system is that for a given area of the country it is
possible to assess in quantitative to semi-quantitative terms the
relative water quality (trophic state) of various water bodies.
Lee (1974b)  has utilized this approach to predict the relative
water quality of a proposed impoundment, compared to other lakes
and impoundments in south-central Wisconsin.

     It should be noted that trophic state in a limnological sense
is not directly translatable to water quality.  Highly fertile
water bodies in which the fertility is manifested in macrophyte
growth could have a relatively low trophic state index based on
the parameters normally used in the relative ranking schemes.

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               Table 33.  PIWONI AND LEE TROPHIC STATE
                          INDEX PARAMETERS
       Parameters
        Description
 1.  Secchi Depth

 2.  Chlorophyll a



 3.  DO Depletion



 4.  Winter Orthophosphate


 5.  Summer Orthophosphate


 6.  Winter Total Phosphorus


 7.  Summer Total Phosphorus


 8.  Winter Total Inorganic
       Nitrogen

 9.  Summer Total Inorganic
       Nitrogen
10 .  Organic Nitrogen
Mean of all values obtained.
Average concentration in first 2
  meters of water column during
  study period.
Percent of lake volume with less
  than 0.5 mg DO/1; May to October,
  inclusive.
Average in-lake concentration dur-
  ing winter under the ice.
Average epilimnion concentration;
  May to October, inclusive.
Average in-lake concentrations dur-
  ing winter under the ice.
Average epilimnion concentrations;
  May to October, inclusive.
Average in-lake concentration dur-
  ing winter under the ice.
Average epilimnion concentration;
  May to October, inclusive.
Average concentration in first 2
  meters of water column during
  study period.
 Taken from Piwoni and Lee (1975).
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Yet it could still have very poor water quality, if water quality
is assessed in terms of impairment of beneficial uses such as
swimming, boating, fishing, etc.   As discussed in another section
of this report, great caution should be exercised in attempting
to translate the impairment in water quality associated with
a given level of chlorophyll or Secchi depth from one part of
the US to another.  The response of the public to various degrees
of algal productivity is highly subjective and regional in
character (Lee, 1
Rast and Lee Trophic Status Index Systems

     Several approaches have been used in this study to develop a
trophic index system based on the Vollenweider phosphorus loading
and mean depth/hydraulic residence time relationship (Figure 19).
One approach is based on the ratio of the current phosphorus load-
ing to the permissible phosphorus loading, the latter as defined
on the Vollenweider phosphorus loading diagram for a given mean
depth/hydraulic residence time value (Figure 19).  This approach
was chosen because it reflects the amount of change in phosphorus
loading necessary to attain a permissible phosphorus load for a
water body with a given mean depth/hydraulic residence time rela-
tionship.  Another approach was developed which relates the per-
missible and excessive phosphorus loads to several water quality
parameters, including chlorophyll a. and Secchi depth.  These
approaches are discussed below.

     The first trophic index classification approach developed in
this study is based on the position of the water bodies on the
Vollenweider phosphorus loading diagram (Figure 19).  It is reason-
able to* suspect that a water body which plots a large vertical
distance above the permissible phosphorus loading line on Vollen-
weider 's diagram is relatively more eutrophic than a water body
which plots a smaller vertical distance above the permissible line,
However, it would be inappropriate to use the linear vertical dis-
tance of a water body above the permissible phosphorus loading
line because of the log-log scale of the Vollenweider diagram.
The simple linear vertical distance from the permissible phos-
phorus loading line would not take into account that water bodies
with high Z/T^ values, and hence in relatively higher phosphorus
loading positions on the Vollenweider diagram, would require a
greater total reduction in phosphorus loads to bring them down to
the permissible phosphorus loading level than would water bodies
with low Z/T  values.
     It should also be noted that since the permissible phosphorus
loading line defines a boundary condition, it may be more appro-
priate to use the perpendicular displacement (i.e., shortest
linear distance) of a water body from the line as a trophic rank-
ing parameter rather than the vertical distance, particularly for
water bodies with high Z/TW values.  However, from the point of

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view of water quality management, the phosphorus loading (i.e.,
y-axis) is the only parameter among the Vollenweider criteria
which can be controlled or managed by man.  Normally, man has
limited opportunity to control or manage the mean depth and
hydraulic residence time of a water body.   Therefore, the dis-
placement of a water body along the y-axis (i.e., phosphorus
loading) of the Vollenweider phosphorus loading diagram (Figure
19) is the parameter of concern in the Rast and Lee trophic
status index.

     This approach involves a determination of the magnitude of
change in water quality one could expect to occur for a given
change in phosphorus loading from the permissible loading level.
This approach assumes that the degree of eutrophy of a water
body is proportional to its phosphorus loading (i.e., phosphorus
limits algal growth in the water body).  While this is true for
many water bodies, there are some water bodies in which phyto-
plankton growth is dependent on other factors such as nitrogen
load.  Under these conditions, the above statements would not be
true over the complete range of phosphorus loads under condi-
tions where phosphorus loads control phytoplankton growth.

     This trophic index system was developed by examining whether
a water body, with a certain phosphorus loading and chlorophyll a_
level, would experience a proportional change in water quality
for a given change in phosphorus load.  This can be determined
by examining whether the magnitude of the phosphorus loading for
a water body above the permissible phosphorus loading is matched
by a proportional difference in chlorophyll a_ above a permissible
level.  As indicated in a following section of this report, the
chlorophyll a concentration corresponding to the permissible
phosphorus loading line on the Vollenweider diagram (Figure 19)
is approximately 2 yg/1 (Vollenweider, 1975a; Dillon and Rigler,
1974a; Jones and Bachmann (1976).  The ratio of the current
phosphorus loads to the permissible phosphorus load, as defined
on the Vollenweider phosphorus diagram (Figure 19) for a given
mean depth/hydraulic residence time value, was used in the  trophic
index.  Thus, a ratio greater than one represents the excessive
phosphorus loading above a certain critical phosphorus loading
level for the eutrophic US OECD water bodies.  Conversely,  a ratio
less than one represents a water body which is not receiving a
"permissible" phosphorus load, relative to its mean depth/
hydraulic residence time characteristics.

     These ratios can be related to water quality parameters,
namely chlorophyll a, Secchi depth, and hypolimnetic oxygen
depletion, in order to provide trophic rankings for different
water bodies.  The validity of this approach stems from the fact
that it has been shown in this investigation that the phosphorus
load to US OECD water bodies can be highly correlated with these
three parameters.  These parameters are generally considered as
being highly indicative of planktonic algal growth and eutrophi-
cation-related water quality.

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     The Rast and Lee trophic index system is similar in several
respects to that of Carlson.  Emphasis is placed on utilization
of parameters of eutrophication (i.e.  chlorophyll and Secchi
depth) to which the public can generally relate.  This is espe-
cially true for water clarity (i.e. Secchi depth).  An important
difference between the Carlson approach and this approach is
that Carlson develops his trophic state index system around
response parameters (i.e., chlorophyll, Secchi depth and total
phosphorus).  These reviewers utilized an excess nutrient load-
ing parameter (i.e., phosphorus) as a means of classifying the
relative trophic status of water bodies.

     It would be of interest to develop e relationship which
directly relates Vollenweider's (1975a) phosphorus loading and
mean depth/hydraulic residence time diagram (Figure 19) to
measurable water quality parameters, as was done with his phos-
phorus loading characteristics and chlorophyll a diagram (Figure
22).  The development of such a model is  discussed below.

     This model or trophic index development centers around the
loading relationship (Equation 9) which serves as the basis for
the permissible phosphorus loading level in the Vollenweider
diagram (Figure 19).  This equation is presented below in a
steady state form suitable for development of this approach:

        L(P) = [P]  z(p +a )                               (49)
                  «    «  p                                    2
     where L(P) = surface area total phosphorus loading  (mg P/m /yr)

           z = mean depth (m),
          p  = hydraulic flushing rate (yr~ ) = 1/T ,
          T  = hydraulic residence time (yr) = water body
           w              3         .                3
                 volume (m )/annual inflow volume (m /yr),
          o  = sedimentation coefficient for phosphorus  (yr~ ),
     and [P]  = steady state phosphorus concentration.
The same assumptions as noted for Vollenweider's model (Vollen-
weider, 1975a) apply to this approach.  In derivation of the
permissible loading line in his loading diagram^ Vollenweider
(1975a; 1976a) chose Sawyer's (1947) spring overturn phosphorus
concentration (i.e., 10 yg/1) as the steady state phosphorus
concentration in the above equation.  The permissible loading
line denotes the phosphorus loading, as a function of the mean
depth/hydraulic residence time characteristics of a water body,
which will produce a spring overturn phosphorus  concentration of
10 ug/1 under steady state conditions.

                                332

-------
     However, it is not mandatory that a steady state phosphorus
concentration of 10 yg/1 be used in Equation 49.   Vollenweider
chose this value "for simplicity" as a meaningful reference point
around which to base boundary conditions.  Other steady state
phosphorus concentrations may also be used in Equation 49 to
produce new phosphorus loading boundary conditions.  The new
boundary condition will no longer be related to Sawyer's (1947)
spring overturn criteria for denoting oligotrophic versus eutrophic
conditions in water bodies.  Instead, the new "permissible" load-
ing level will be the phosphorus loading which will produce the
new steady state phosphorus concentration which was inserted into
Equation 49 .

     The basis for the modification of Equation 49 in this study
to relate the loading lines on the Vollenweider diagram to water
quality parameters is based on earlier work by Sakamoto (1966),
Dillon and Rigler (1974a) and Jones and Bachmann (1976).  Dillon
and Rigler (1974a; 1975), elaborating on earlier work by Sakamoto,
investigated the hypothesis that a power relationship existed
between chlorophyll and phosphorus in many water bodies.  They
correlated the summer mean chlorophyll a concentration (as a
measure of the algal biomass ) in a water body with its spring
overturn phosphorus concentration.  Their data base (n=77) also
included that of Sakamoto (1966) plus a number of literature
values.  The result was the regression equation:
log1Q [chlorophyll
       =  1.45  log
                                          1Q
                                                           (50)
           r , n    TIT  -, summer               , n    ,  -, -,
     where [chlorophyll aj,      = summer mean chlorophyll a
                        ~                              3  ~
                                    concentration (mg/m ) , and
[P],
   A
                                = spring overturn mean total
                                    phosphorus concentration

                                   • (mg/m ) .
The correlation coefficient was r=0.96, indicating a very strong
relationship between these two parameters.  Jones and Bachmann
(1976) did a similar analysis on lakes in Iowa plus a larger num-
ber of literature values.  Interestingly, Jones and Bachmann
regressed the summer mean chlorophyll concentration on the summer
mean total phospohrus , rather than the spring overturn total phos
phorus .  However, they obtained an almost identical regression
equation and correlation coefficient :
log1Q [chlorophyll
                                 = 1.46 Io
                  10
                                                   _ 1>Q9
                               r = 0.95

This indicates a water body's total phosphorus concentration
appears to remain relatively constant over the annual cycle .
Such an occurrence was demonstrated by Lee et al .  (1976) in
studies on Lake Mendota.
                               333

-------
     One may incorporate the work of Dillon and Rigler (1974a;
1975) and Jones and Bachmann (1976) into Vollenweider ' s equation
for the permissible and/or excessive phosphorus loading levels
to produce boundary conditions manifested in a water quality
parameter, namely phosphorus concentration.  Equation 50 above
can be rearranged as :
     Iog10 [P]SP   = Iog10 [chlorophyll a]SUmmer + 1.14^   (52)
                                __^                    »


Equation 49 can be arranged in the same manner as :


     log  [P]summer= log1Q [chlorophyll a]summer> + 1>09    (53)
        10   A     ~            j-^ - •


One can solve these equations for the phosphorus concentrations
which will produce a given summer chlorophyll a_ concentration.
A useful point about the above equations is that one can solve
them to obtain the relationship between as many total phosphorus
and chlorophyll a concentrations as desired.  The  result will be
a sequence of different total phosphorus and chlorophyll a data
sets .                                                    ~

     The final step in the development of this approach is to use
either Equation 52 or 53,  or the mean value of both equations,
and Equation 49 to translate Vollenweider ' s permissible and exces-
sive loading lines (Figure 19) into expected chlorophyll a_ con-
centrations.  This can be  done by the use of Equations 52~and/or
53 to determine the total  phosphorus concentration required to produce
a given summer epilimnetic chlorophyll a_ concentration.  The resultant
phosphorus concentration can be inserted into Equation 49, which
can then be solved for the particular phosphorus loading necessary
to produce the inserted phosphorus concentration.   In addition,
the phosphorus concentration has also been related to a chlorophyll
a concentration (Equations 50 and/or 51).  Thus, the solution of
Equation 49 for a given steady state phosphorus concentration also
directly relates the phosphorus loading to a given chlorophyll a_
concentration.  One can then also relate these boundary lines to
Secchi depth and hypolimnetic oxygen depletion with the use of
Equations 38 and 41, respectively.  With the use of these equations
Vollenweider ' s phosphorus  loading diagram (Figure  19) can be
transformed so as to relate phosphorus loads to summer chlorophyll,
Secchi depth and hypolimnetic oxygen depletion conditions in a
water body.  The permissible and excessive phosphorus loading
lines correspond, based on spring overturn phosphorus concentra-
tions of 10 yg/1 and 20 yg/1, respectively (Sawyer, 1947;
Sakamoto, 1966; Dillon and Rigler, 1975), to summer mean
epilimnetic chlorophyll a concentrations of 2 yg/1 and 6 yg/1,
to mean Secchi depths of 4.6 m? and 2.7 m and hypolimnetic oxygen
depletion rates of 0.3 mg 02/m^/day and 0 . 6 mg 0^/m2/day, re-
spectively.  The results of the above approach are presented in
a following section.
                                 334

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TROPHIC STATUS INDICES AS APPLIED TO THE US OECD WATER BODIES

US EPA Trophic Status Index System

     The US EPA  (1974d) Trophic  State  Index parameters were  listed
in Table 31.  Because the minimum dissolved oxygen  concentration
was  not available  for most of  the US OECD water bodies,  this para-
meter (i.e., 15 minus the dissolved oxygen concentration) was not
included in the final ranking  number.  While this means  that the
final ranking of the US OECD water bodies is based  only  on five
of the six US EPA  Trophic State  Index  parameters, it should still
give a reasonably  accurate relative trophic state ranking of the
US OECD water bodies.  For the purpose of this discussion, the
US EPA approach is described as  "modified" Commission of DO value)
from the classification scheme.    In general, the data used in the
US EPA Trophic State Index, as well as that of Carlson,  Piwoni
and Lee, and Rast  and Lee, was taken from the US OECD Summary
Sheets (Appendix II) in this report.   However, Rast and  Lee also
made use of Vollenweider's phosphorus  loading diagram (Figure 19).

     The relative  ranks of the US OECD water bodies based on the
five US EPA trophic state index  parameters used in  this  classifi-
cation effort are  presented in Table 34.  In this system, the
water bodies with  the lowest trophic status index number are
relatively the most productive,  while  the least productive lake in
the series will have the highest trophic status index number.  The
US EPA (1974d) used the percent  of the lakes in their study which
exceeded a parameter value for each lake to produce the  relative
ranking for each lake for a given trophic state index parameter.
The same method was used by these reviewers, but the actual
number of lakes, rather than the percent exceeding  a parameter
value for a particular lake, was used  in the ranking.  The relative
ranking position of the water  bodies is identical in both cases.
Water bodies with  identical parameter  values (ties) were given the
same ranking number.  It should  be noted that all parameter values
were not available for all US  OECD water bodies.

     The trophic status rankings of the US OECD water bodies,
using the modified US EPA criteria, are presented in Table 35.
Since no trophic condition has been associated with a particular
Trophic Status Index Number(s),  the trophic ranking is by necessity
only a relative ranking.  In general,  the relative  trophic rank-
ing of the US OECD water bodies  is as  expected based on  the
relative general characteristics of the water bodies.  There are,
however, several anomalies in  the ranking, based on the  trophic
conditions reported by the US  OECD investigators.   Particularly,
Lakes Harriet, Washington-1957,  Calhoun, and Shagawa appear to be
higher in the ranking (i.e., more toward the oligotrophic end of
the scale)  than expected.   Conversely, it would be  said  that Lakes
Cayuga and Weir are lower in the ranking than would be expected,
relative to the reported trophic conditions for the other water
bodies in the ranking.   These  apparent anomalies will be addressed
in more detail in a following section.
                                335

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                                   Table  34.   RANKING OF US  OECD  WATER BODIES USING

                                              MODIFIED US EPA  TROPHIC  STATE INDEX SYSTEM
GO
co
Relative Ranking Under Indicated Parameter:
Total
Phosphorus
Water Body (mg/1)
Blackhawk (E)a
Brownie (E)
Calhoun (E)
Camelot-Sherwood (E)
Canadarago (E)
1968
1969
Cayuga (M)
1972
1973
Cedar (E)
Cox Hollow (E)
Dogfish (0)
1971
1972
Dutch Hollow (E)
George (0-M)
Harriet (E)
Isles (E)
Kerr Reservoir (E-M)
Roanoke Arm
Nutbush Arm
llb
_
8C
29b

26
28

36
36
24C
16b

44
44
3b
46
20C
7C

32
32
Inorganic
Nitrogen
(mg/l)e
6
39C'f
33°'f
4

18
15

20
12
39c'f
8

17
_
16
U0f
39°'f
39C'f

22
25
500-Secchi
Depth
( inches)
43
14
24
23

20
20

33
33
20
14

36
35
2
45
34
3

14
6
Dissolved
Chlorophyll a Phosphorus
(pg/1) (mg/1)
191
35C
34C
291

22
28

34
38
15°
81

34
39
41
_
40C
2C

20
12
11
25
34
27

19
19

38
36
34
10

-
_
20
40
34
25

23
19
Trophic Status
Index Number
(Sum of
Rankings)
90
_
133
112

105
110

161
155
132
56

-
_
45
_
167
76

111
94

-------
                                 Table  34  (continued).
                                        RANKING OF US OECD WATER BODIES USING
                                        MODIFIED US EPA TROPHIC STATE INDEX SYSTEM
CO
CO
Relative Ranking Under Indicated Parameter:
Water Body
Lamb (0)
1971
1972
Meander (0)
1971
1972
Mendota (E)
Total
Phosphorus
(mg/1)

40
42

42
45
4
Inorganic
Nitrogen
(mg/l)e

12
-

13
-
7
500-Secchi
Depth
( inches )

20
27

40
39
39
Chlorophyll a
(Ug/l)

34
41

38
43
26
Dissolved
Phosphorus
(mg/1)

-
_

-
_
3
Trophic Status
Index Number
(Sum of
Rankings)

_
_

-
_
79
Michigan
  Openwaters - 1971 (0) 39
  Nearshore
   Waters - 1971(M)     38
Lower Minnetonka
  1969 (E)              23
  1973 (E-"M)            26
Potomac Estuary (U-E)
  Upper Reach            0°
  Middle Reach           ld
  Lower Reach           27
Redstone (E)            17b
Sallie (E)               2
Sammarftish (M)           32
Shagawa (E)             23
Stewart (E)             23*
30

27
                                                                33
                                                                14
                                                                20
                                                    24U
                                                    34d
                                                    12
                                                    15
                                                    29®
                                                    31
                                                     0
            16

            41
            33
43

38

13
25
18
21J
                         18
                         23d
42

41


38
              27
               1
              29
              14
              36
                                                                                                        187
              I
             31
            107
             93
            119
             90

-------
                                 Table  34  (continued).
RANKING OF US OECD WATER BODIES USING

MODIFIED US EPA TROPHIC STATE INDEX SYSTEM
CO
CO
oo
Relative Ranking Under Indicated Parameter:
Water Body
Tahoe (U-0)
East Twin
1972 (E)
1973 (E)
1974 (E)
West Twin
1972 (E)
1973 (E)
1974 (E)
Twin Valley (E)
Virginia (E)
Waldo (U-0)
Washington
1957 (E)
1964 (E)
1971 (M)
1974 (M)
Weir (M)
Wingra (E)
Total
Phosphorus
(mg/1)
47

16
16
16

5
7
9
19b
llb
48h

33
18
37
-
16
36
Inorganic
Nitrogen
(mg/l)e
41

9
2
-

5
4
_
20
27
42h

32
24
29
-
35
21f
500-Secchi
Depth
(inches)
47

16
33
22

27
37
33
14
6
46h

27
6
42
44
22
7
Chlorophyll a
(ug/1)
45^

9
11
7

3
10
7
161
51
44h

25
15
34
-
27
^
Dissolved
Phosphorus
(mg/1)
34

10
7
19

5
5
7
22
22
34h

40
10
29
_
14
19
Trophic Status
Index Number
(Sum of
Rankings)
214

60
69
-

45
63
_
91
71
214

157
73
171
_
liu
—

-------
                            Table 34 (continued).  RANKING OF US OECD WATER BODIES USING

                                                   MODIFIED US EPA TROPHIC STATE INDEX SYSTEM


                                            Relative  Ranking Under Indicated Parameter:         Trophic Status
                                    TotalInorganic500-SecchiDissolved   Index Number
                                  Phosphorus   Nitrogen     Depth     Chlorophyll a  Phosphorus    (Sum of
            Water  Body             (mg/1)       (mg/l)e    (inches)      (wg/1)   ~    (mg/1)      Rankings)

              EXPLANATION:

                 Investigator-indicated trophic condition
                     E  =  eutrophic
                     M  =  mesotrophic
                     0  =  oligotrophic
                     U  =  ultra

                 Based  on mean  of summer and winter concentrations.
                Q
                 Based  on mean  summer surface values.

                 Based  on mean  summer values.
oj               e            +
co                Based  on NH4+MO.+NO- (as N) unless otherwise indicated.
CD                               J    *•
                fBased  on NH*+NO~ (as N) values.

                gBased  on N0~+N0~ (as N) values.

                'Based  on August values from 1970 to 1974.

                Based  on samples from upper two meters of water column.

                JBased  on euphotic zone measurements.

                Dash  (-)  indicates data not available.

-------
         Table 35.  RELATIVE TROPHIC STATUS RANKING
                    OF US OECD WATER BODIES USING MODIFIED
                    US EPA TROPHIC STATUS INDEX SYSTEM.
           Water Body
                      Investigator-Indicated
                          Trophic Status
                         Trophic Status
                          Index Number
tie
tie
TTahoe
|^ Waldo
 Michigan
  Open Waters - 1971
 Washington - 1971
 Harriet
 Cayuga - 1972
 Washington - 1957
 Cayuga - 1973
 Calhoun
 Shagawa
 Weir
 Camelot - Sherwood
 Kerr - Roanoke Arm
 Canadarago - 1969
 Potomac - Lower
 Reach
 Canadarago - 1968
 Kerr - Nutbush Arm
 Redstone
 Twin Valley
JBlackhawk
\.Stewart
 Mendota
 Isles
 Washington - 1964
 Virginia
 East Twin -  1973
 West twin -  1973
ultra - oligotrophic
ultra - oligotrophic
oligotrophic

mesotrophic
eutrophic
mesotrophic
eutrophic
mesotrophic
eutrophic
eutrophic
mesotrophic
eutrophic
eutrophic - mesotrophic
eutrophic
eutrophic
eutrophic
eutrophic
eutrophic
eutrophic
eutrophic
eutrophic
eutrophic
eutrophic
eutrophic
eutrophic
eutrophic
eutrophic
                                       - mesotrophic
214
214
187

171
167
161
157
155
133
119
114
112
111
110

107
105
 94
 93
 91
 90
 90
 79
 76
 73
 71
 69
 63
                                 340

-------
    Table  35  (continued)
                   RELATIVE TROPHIC STATUS RANKING
                   OF US OECD WATER BODIES USING MODIFIED
                   US EPA TROPHIC STATUS INDEX SYSTEM
           Water Body
                     Investigator-Indicated
                         Trophic Status
                         Trophic Status
                          Index Number
tie
East Twin - 1972
Cox Hollow
: Dutch Hollow
West Twin - 1972
Potomac - Middle
Reach
Potomac - Upper
Reach
eutrophic
eutrophic
eutrophic
eutrophic

ultra-eutrophic

ultra-eutrophic
60
45
45
45

31

 1
                                 341

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Carlson Trophic Status Index System

     The parameters in Carlson's (1974) Trophic Status Index
were listed in Table 32.   An absolute TSI value can be assigned
to a water body on the basis of either its phosphorus or chloro-
phyll concentration and/or its Secchi depth.   However, the
trophic rankings are still relative, as with the US EPA Trophic
Status Index System, since no TSI value or range was assigned to
a given trophic condition in Carlson's system.  If it were neces-
sary to assign a TSI value to a given trophic condition, general
limnological knowledge would suggest that a reasonable boundary
value between eutrophic and oligotrophic might be a TSI value of
40.  This TSI value would indicate a Secchi depth of 4 meters, a
chlorophyll concentration of 2.6 ug/1 and a phosphorus concen-
tration of about 16 ug/1.  This value as a boundary condition is
based solely on the experience of these reviewers.

     The relative rankings of the US OECD water bodies, based on
their phosphorus and chlorophyll concentrations, and Secchi depths,
are presented in Table 36.  Inspection of Table 36 indicates that
the relative positions of the US OECD water bodies vary widely,
depending on the particular Carlson TSI parameter examined.

     In order to demonstrate the relationship between the three
Carlson TSI parameters, the US OECD water bodies were ranked by
these parameters on the basis of increasing productivity or
eutrophy.  In this ranking the order of water bodies is from the
oligotrophic end of the trophic scale to the eutrophic end, with
the relatively most eutrophic water body listed first.  The re-
sults are presented in Table 37 (the investigator-indicated
trophic states were indicated in Table 36).

     A general inspection of Table 37 shows that while the ultra-
oligotrophic and ultra-eutrophic water bodies are listed at the
appropriate ends of the ranking scales, there are a number of
differences in the relative positions of US OECD water bodies
using the three different Carlson TSI parameters.  For example,
the reported Secchi depths for Lakes Blackhawk, Mendota, and
Harriet place them higher (i.e., toward the oligotrophic end of
the scale) in the relative ranking than several other water bodies
generally considered less productive (i.e., Washington - 1971,
Dogfish and Cayuga, respectively).  The chlorophyll concentrations
for Lakes Harriet, Brownie and Calhoun also place them higher in
the relative ranking than less productive Lakes Cayuga, Dogfish
and Lamb.

     Carlson (1974), using data for Lake Washington, has demon-
strated that the data for this lake and TSI values follow the same
trends and that they produce the same relative values when trans-
formed to the trophic scale.  He has also indicated that the TSI
values (and relative rankings) are not always identical.  Such an
anomaly can be used as an internal check on the assumptions being

                                342

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Table 36.  RANKING OF US OECD WATER BODIES USING
           CARLSON TROPHIC STATUS INDEX SYSTEM

Water Body ''
Blackhawk (E)a
Brownie (E)
Calhoun (E)
Came lot -Sherwood (E)
Canadarago (E)
1968
1969
Cayuga (M)
1972
1973
Cedar (E)
Cox Hollow (E)
Dogfish (0)
1971
1972
Dutch Hollow (E)
George (0-M)
Harriet (E)
Isles (E)
Kerr Reservoir (E-M)
Roanoke Arm
Nutbush Arm
Lamb ( 0 )
1971
1972
Meander (0)
1971
1972
Mendota (E)
Relative Ranking Under Indicated Parameter:
rSI(SD)
5
33
24
25
.
28
15
15
28
33
12
13
45
3
14
44
33
41
28
21
8
9
9
TSI(TP)
35b
—
37°
19b
22
20
13
13
24C
31b
5
5
43b
3
28°
38
16
16
9
7
7
4
42
TSH Chlorophyll)
26f
12
13°
18f
25
19
13
8
29C
37f
13
7
41f
6C
43C
26
33
13
5
8
3
21
                        343

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Table 36(continued).   RANKING OF US OECD WATER
        BODIES USING CARLSON TROPHIC INDEX SYSTEM

Relative Ranking Under Indicated Parameter:
Water Body
Michigan (0-M)
Nearshore Waters -
1971
Open Waters -
1971
Lower Minnetonka
1969 (E)
1973 (E+M)
Potomac Estuary (U-E)
Upper Reach
Middle Reach
Lower Reach
Redstone (E)
Sallie (E)
Sammamish (M)
Shagawa (E)
Stewart (E)
Tahoe (U-0)
East Twin
1972 (E)
1973 (E)
1974 (E)
West Twin
1972 (E)
1973 (E)
1974 (E)
Twin Valley (E)
Virginia (E)
Waldo (U-0)
TSI(SD)

15
__

33
28
47d
45d
33d
31
-
7
15
33
1

31
15
26

21
11
15
33
41
26
TSKTP)

11
9

25
22
46d
45d
21d
30b
44
16
25
25b
1

31
31
31

41
38
38
29b
35b
2e
TSKChlorophyll)

8
3

32
22
45d
44d
2yd
25f
-
8
27
24f
is

36
34
38

42
35
38
29f
40f
2e »g
                         344

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        Table 36(continued).  RANKING OF US OECD WATER
                BODIES USING CARLSON TROPHIC INDEX SYSTEM

Relative Ranking Under Indicated Parameter:
Water Body
Washington
1957 (E)
1964 (E)
1971 (M)
1974 (M)
Weir (M)
Wingra (E)
TSI(SD)
21
41
6
4
26
40
TSI(TP)
15
29
12
-
31
13
TS I (Chlorophyll)
22
29
13
-
20
—
EXPLANATION:

 Investigator-indicated trophic status:
     E = eutrophic
     M = mesotrophic
     0 = oligotrophic
     U = ultra
 Based on mean of summer and winter concentrations.
°Based on mean summer surface values.
 Based on mean summer values.

6Based on August values from 1970 to 1974.
 Based on samples from upper two meters of water column.
Q~
&Based on euphotic zone measurements.

Dash (-) indicates data not available.
                                  345

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        Table 37.  RELATIVE  TROPHIC STATUS RANKING OF US
                   OECD WATER  BODIES USING CARLSON TROPHIC
                   STATUS  INDEX  SYSTEM
        TSI(SD)
        TSI(TP)
    TSI(Chlorophyll)
    Tahoe
    Waldo
    George
    Washington-1974
    Blackhawk
    Washington-1971
    Sammamish
    Meander-1971
   fMeander-1972
   \Mendota
    West Twin-1973
    Dogfish-1971
    Dogfish-1972
    Harriet
   ^tayuga-1972
    Cayuga-1973
    Michigan
tie\
tie
tie< .
tie
      Open Waters - 1971
 Tahoe
 Waldo
 George
 Meander-1972
 Dogfish-1971
 Dogfish-1972
•^
fLamb-1972
 Meander-1972
 Lamb-1971
 Michigan           , .  ,
   Open Waters-1971    \
 Michigan , Nearshore
   Waters-1971
 Washington-1971
fCayuga-1972
^Cayuga-1973
 Washington-1957
/rCerr-Roanoke Arm
                           Tahoe
                           Waldo
                           (Meander-1972
    Shagawa
    East Twin-1973
    West Twin-1974
    v
    fLamb-1972
tie(West Twin-1972
    lwashington-1957
    Calhoun
    Camelot-Sherwood
    (East Twin-1974
tie(Kerr-Nutbush Arm
   vSammamish
    Camelot-Sherwood
    Canadarago-1969
    Potomac-Lower
      Reach
   rCanadarago-1968
tieS
   I Lower Minnetonka-
      1973
    Cedar
tie/.,. , .
   NMichigan
    L Open Waters-1971
    Lamb-1972
    Harriet
    Dogfish-1972
    *•
    Cayuga-1973
    Meander-1971
    Michigan, Nearshore
      Waters-1971
    Sammamish
    W
    Brownie
    (Calhoun
    Cayuga-1972
tie{Dogfish-1971
    Lamb-1971
    Washington-1971
    Camelot-Sherwood
    Canadarago-1969
    Weir
    Mendota
    /Lower Minnetonka-
tie/  1973
    (Washington-1957
    Stewart
    Canadarago-1968
                                  346

-------
        Table  37(continued)   RELATIVE TROPHIC STATUS RANKING
                 OF THE  US  OECD  WATER BODIES USING CARLSON
                 TROPHIC STATUS  INDEX SYSTEM
         TSI(SD)
         TSI(TP)
                           TSK Chlorophyll)
    (Canadarago-1969
    I Cedar
tie^Lamb-1971
    [Lower Minnetonka-
    v> 1973
    (Redstone
    East Twin-1972
    Brownie
    (Cox Hollow
    jKerr-Roanoke Arm
    Lower Minnetonka-
      1969
    Potomac-Lower
      Reach
    JWin Valley
    Stewart
    Wingra
    /Kerr-Nutbush Arm
tie< Virginia
    lwashington-1964
    Isles
    /Dutch Hollow
tle\Potomac-Middle
    L.  Reach
    Potomac-Upper
      Reach
   [Lower Minnetonka-
      1969
tiesShagawa
   (Stewart
    Harriet
    Twin Valley
    [c.
    IE,
tie £
   IE,
    Redstone
    S
    Cox Hollow
    East Twin-1972
    East Twin-1973
    East Twin-1974
   (^Blackhawk
tie
-------
made about a water body's utilization of phosphorus for planktonic
algal growth.  To cite an example (Carlson, 1974), if a water body
has a higher TSI(TP) than its TSI(SD) and TSI (Chlorophyll), and
the latter two values are similar, then it may indicate that the
water body is not phosphorus-limited.

Piwoni and Lee Trophic Status Index System

     The Trophic State Index parameters of Piwoni and Lee were
presented in Table 33.  As with the US EPA (1974d) Trophic State
Index System, all parameter values were not available for all US
OECD water bodies.  As before, if a water body did not have values
for all the Piwoni and Lee Trophic State Index parameters, it was
not included in the final ranking.  Further, the parameters used
by these reviewers in ranking the US OECD water bodies using the
Piwoni and Lee system were altered so that available data could be
used.  Phosphorus and nitrogen values were not reported on a
seasonal basis in most cases.  Also, the DO depletion was unavail-
able for most US OECD water bodies.  The result was that the
Secchi depths, total and dissolved phosphorus, and inorganic
nitrogen concentration, and chlorophyll a concentration of the
US OECD water bodies were used to rank them in the Piwoni and Lee
Trophic Status Index System.  The rankings of the US OECD water
bodies using the modified Piwoni and Lee parameters, are presented
in Table 38.

     The relative ranks of the US OECD water bodies, based on the
five modified Piwoni and Lee Trophic State Index parameters, are
presented in Table 39.  In this table, the more oligotrophic water
bodies are listed first.  As with the other relative trophic rank-
ings , there is general agreement between the US OECD water body's
relative trophic rankings and the trophic conditions indicated by
their respective investigators.  Lake Harriet occupies a higher
relative ranking than less productive Lakes Washington - 1974 and
Cayuga, while Lake Weir occupies a. lower relative ranking than
more productive Lakes Cedar, Shagawa and Calhoun.  Lake Shagawa,
based on limnological characteristics, also occupies a higher
ranking than less productive water bodies.

Rast and Lee Trophic Status Index System

     Eor this discussion, these authors chose several of the same
trophic state indicators used in Carlson's (1974) Trophic Status
Index System; namely Secchi depth and chlorophyll a.  The ranking
of the US OECD water bodies, based on their current phosphorus
loading/permissible phosphorus loading and current chlorophyll/per-
missible chlorophyll quotients, Secchi depth and chlorophyll a
concentrations as ranking parameters, is presented in Table 40.
The relative rankings of the US OECD water bodies , based on the
above-mentioned parameters, are listed in Table 41.
                                348

-------
                                  Table 38 .   RANKING OF US OECD WATER BODIES USING
                                             PIWONI AND LEE MODIFIED TROPHIC STATUS
                                             INDEX SYSTEM
co
-tr

Relative Ranking Under Indicated Parameter: Trophic Status
Total
Phosphorus
Water Body (mg/1)
Blackhawk (E)a
Brownie (E)
Calhoun (E)
Camelot-Sherwood (E)
Canadarago (E)
1968
1969
Cayuga (M)
1972
1973
Cedar (E)
Cox Hollow (E)
Dogfish (0)
1971
1972
Dutch Hollow (E)
George (0-M)
Harriet (E)
Isles (E)
Kerr Reservoir (E-M)
Roanoke Arm
Nutbush Arm
39g
_
426
20g

23
21

13
13
25e
336

5
5
47g
3
29e
43e

17
17
Inorganic
Nitrogen
(mg/1) h
36g
4e,i
II6'1
38g

25
28

23
31
4e,i
34g

26
_
27g
31
4e,i
^,i

21
18
Secchi
Depth
(m)
5
34
24
25

28
-

15
15
28
34

12
13
46
3
14
45

34
42
Dissolved
Chlorophyll a Phosphorus
(yg/1) ~ (mg/1)
27d
lle
12e
17d

24
18

12
8
316
38d

12
7
42d
_
66
44fi

26
34
32g
18S
9e
16g

24
24

5
7
9e
33g

-
_
23g
3
9e
18S

20
24
Index Number
(Sum of
Rankings)
139
-
98
116

124
-

68
74
97
172

-
-
185
-
62
154

118
135

-------
                                    Table  38(continued).   RANKING OF US OECD WATER BODIES USING
                                            PIWONI AND  LEE MODIFIED  TROPHIC  STATUS INDEX SYSTEM
OJ
en
O

Relative Ranking Under Indicated Parameter: Trophic Status
Total Inorganic Secchi
Phosphorus Nitrogen Depth
Water Body (mg/1) (mg/1) h (m)
Lamb ( 0 )
1971
1972
Meander (0)
1971
1972
Mendota (E)
Michigan
Nearshore Waters (M)
-1971
Open Waters (0)
-1971
Lower Minnetonka
1969 (E)
1973 (E-M)
9 31 28
7 - 21
7 30 8
4-9
46 35 9
11 16
10 13 15
26 - 34
23 - 28
Chlorophyll a
(Mg/D ~
12
5
8
3
20
8
3
33
21
Dissolved Index Number
Phosphorus (Sum of
(mg/1) Rankings)
-
-
-
-
46 156
2
1 42
_
5
                Potomac  Estuary  (U-E)     ^
                 Upper  Reach           50
                 Middle Reach         49
                 Lower  Reach           22
                Redstone (E)            32g
                Sallie  (E)              48
                Sanunamish (M)            17
                Shagawa  (E)             26
                Stewart  (E)             26g
42
19
10
28
13
m«
48
46l
341
32
             7
            15
            40
                          461
                          451
                          281
                          25C
              28
              23C
43
40
30
                           42
                           14
                           29
229
199
124
137

 60
111
137

-------
                                 Table 38(continued).  RANKING OF US OECD WATER BODIES USING
                                         PIWONI AND LEE  MODIFIED TROPHIC STATUS  INDEX SYSTEM
CO
en

Relative Ranking Under Indicated Parameter: Trophic Status

Water Body
Tahoe (U-0)
East Twin
1972 (E)
1973 (E)
1974 (E)
West Twin
1972 (E)
1973 (E)
1974 (E)
Twin Valley (E)
Virginia (E)
Waldo (U-0)
Washington
1957 (E)
1964 (E)
1971 (M)
1974 (M)
EXPLANATION:
a_ . . ...
Total
Phosphorus
(mg/1)
1

33
33
33

45
43
41
3QS
39^
2C

16
31
12
13

Inorganic
Nitrogen
(mg/l)h
2

33
40
-

37
38
_
23g
16^
lc

12
19
14
221

Secchi
Depth
(m)
1

32
15
26

21
11
15
34
42
2c

21
42
6
41


Chlorophyll a
(pg/1)
1

37
35
39

43
36
39
30d
41d
2c,f

21
31
12
-

Dissolved
Phosphorus
(mg/1)
9

33
36
24

38
38
36
216
218
9C

3
33
14
24

Index Number
(Sum of
Rankings)
14

168
159
-

184
166
-
138
159
16

73
156
58
-

               Investigator-indicated trophic status
                   E = eutrophic
                   M = mesotrophic
                   0 = oligotrophic
                   U = ultra

-------
                   Table  38(continued).  RANKING OF US OECD WATER BODIES USING
                            PIWONI AND LEE MODIFIED TROPHIC  STATUS  INDEX SYSTEM
                    	Relative  Ranking  Under  Indicated  Parameter:         Trophic Statin
                       Total       InorganicSecchiDissolvedIndex Number
                    Phosphorus    Nitrogen     Depth      Chlorophyll a  Phosphorus    (Sum of
Water Body             (mg/1)       (mg/1)       (m)          (ug/D         (nig/1)       Rankings)

 Based on mean summer  values.
GBased on August values from 1970 to 1974.
 Based on samples taken from upper two meters of water column.
eBased on mean summer  surface values.
 Based on eutrophic zone measurements.
^Based on mean of summer and winter concentrations.
 Based on NH. +N07+NO"  (as N) unless otherwise indicated.
             "   o   L.
1Based on NH^+NO~ (as  N) values.
.            4   J
^Based on N0~+N0~ (as  N) values.

-------
               Table 39 .
                    RELATIVE TROPHIC STATUS RANKINGS
                    OF US OECD WATER BODIES USING
                    PIWONI  AND LEE  MODIFIED TROPHIC
                    STATUS  INDEX  SYSTEM
           Water Body
                        Investigator-Indicated
                            Trophic Status
                               Trophic Status
                                Index Number
tie
tie
 Tahoe
 Waldo
 Michigan
    Open  Waters  -  1971
 Washington  - 1971
 Sammamish
 Harriet
 Cayuga  - 1972
 Cayuga  - 1973
 Washington - 1974
 Cedar
 Calhoun
 Shagawa
 Camelot-Sherwood
 Weir
 Kerr-Roanoke Arm
rCanadarago - 1968
(^Potomac - Lower Reach
 Kerr -  Nutbush Arm
fRedstone
(_ Stewart
 Twin Valley
 Virginia
 Mendota
 Isles
 Washington - 1964
 East Twin - 1973
 West Twin - 1973
ultra-oligotrophic
ultra-oligotrophic

oligotrophic
mesotrophic
mesotrophic
eutrophic
mesotrophic
mesotrophic
mesotrophic
eutrophic
eutrophic
eutrophic
eutrophic
mesotrophic
eutrophic-mesotrophic
eutrophic
eutrophic
eutrophic-mesotrophic
eutrophic
eutrophic
eutrophic
eutrophic
eutrophic
eutrophic
eutrophic
eutrophic
eutrophic
 14
 16

 42
 58
 60
 62
 68
 74
 82
 97
 98
111
116
117
118
124
124
135
137
137
138
144
150
154
156
159
166
                                    353

-------
     Table 39 (Continued).   RELATIVE TROPHIC STATUS RANKINGS
              OF US OECD WATER BODIES USING PIWONI AND LEE
              MODIFIED TROPHIC STATUS INDEX SYSTEM
                       Investigator-Indicated        Trophic Status
    Water Body	Trophic Status	Index Number

East Twin - 1972       eutrophic                          168
West Twin - 1972       eutrophic                          184
Dutch Hollow           eutrophic                          185
Potomac - Middle
Reach                  ultra-eutrophic                    199
Potomac - Upper
Reach                  ultra-eutrophic                    229
                             354

-------
Table 40.  RANKING OF US OECD WATER BODIES
           USING SECCHI DEPTH, CHLOROPHYLL a, EXCESS
           CHLOROPHYLL AND EXCESS PHOSPHORUS LOADING AS
           RANKING PARAMETERS

Relative Ranking Under Indicated Parameters




Water Body

Blackhawk (E)a
Brownie (E)
Calhoun (E)
Camelot -Sherwood (E)
Canadarago (E)
1968
1969
Cayuga (M)
1972
1973
Cedar (E)
Cox Hollow (E)
Dogfish (0)
1971
1972
Dutch Hollow (E)
George (0-M)
Harriet (E)
Isles (E)
Kerr Reservoir (E-M)
Roanoke Arm
Nutbush Arm
Lamb ( 0 )
1971
1972
Mean
Secchi
Depth
(m)


5
33
24
25

-
28

15
15
28
33

12
13
45
3
14
44

33
41

28
21
Mean
Chloro-
phyll a
(yg/D~


26C
12d
13d
18C

25
19

13
8
29d
37°

13
7
41°
_
6d
43d

26
33

13
5
(Current
Chloro-
phyll a ) /
(Permis-
sible
Chloro-
phyll a )
27
14
15
18

19
22

20
13
31
38

7
4
42
_
7
44

26
29

11
7
(Current
Phosphorus
Loading ) /
(Permissible
Phosphorus
Loading )

41
36
32
33

-
20

26
_
17
42

-
2
34
6
28
44

35
30

-
3
                      355

-------
Table 40 (continued).  RANKING OF US OECD WATER BODIES
         USING SECCHI DEPTH, CHLOROPHYLL a, EXCESS
         CHLOROPHYLL AND EXCESS PHOSPHORUS LOADING AS
         RANKING PARAMETERS

Relative Ranking Under Indicated Parameters

Water Body
Meander (0)
1971
1972
Mendota (E)
Michigan (0)
-open waters
(T =30 yrs)
1971
1974
Michigan (0)
-open waters
(T = 100 yrs)
1971
1974
Lower Minnetonka
1969 (E)
1973 (E+M)
Potomac Estuary (U-E)
Upper Reach
Middle Reach
Lower Reach
Redstone (E)
Sallie (E)
Sammamish (M)
Shagawa (E)
Mean
Secchi
Depth
(m)

8
9
9

-
-

-
-

33
28
47b
45b
33b
31
-
7
15
Mean
Chloro-
phyll a
(yg/l)~

8
3
21

3
-

3
-

32
22
45b
44b
27b
25C
-
8
27
(Current
Chloro-
phyll a)/
(Permis-
sible
Chloro-
phyll a)

7
3
31

5
-

5
-

33
21
46
45
28
25

12
35
(Current

Phosphorus
Loading)/
(Permissible
Phosphorus
Loading )

-
3
37

11
8

12
9

25
10
48
47
31
40
46
20
23



















                            356

-------
Table 40 (continued).  RANKING OF US OECD WATER BODIES
         USING SECCHI DEPTH, CHLOROPHYLL a, EXCESS
         CHLOROPHYLL AND EXCESS PHOSPHORUS LOADING AS
         RANKING PARAMETERS





Water Body



Stewart (E)
Tahoe (U-0)
East Twin
1972 (E)
1973 (E)
1974 (E)
West Twin
1972 (E)
1973 (E)
1974 (E)
Twin Valley (E)
Virginia (E)
Waldo (U-0)
Washington
1957 (E)
1964 (E)
1971 (M)
1974 (M)
Weir (M)
Wingra (E)
Explanation :
Investigator -indicated
E = eutrophic
M - mesotrophic
0 = oligotrophic
U = ultra
Relative
Mean
Secchi
Depth
(m)



33
1

31
15
26

21
11
15
33
41
2e

21
41
6
4
26
40

trophic




Ranking Under Indicated Parameters
Mean
Chloro-
phyll a
(yg/l)~~



24°
lf

36
34
38

42
35
38
29C
40C
2e,f

22
29
13
-
20
-

state :




(Current
Chloro-
phyll a)/
(Permis-
sible
Chloro-
phyll a)
24
1

37
34
39

43
36
39
30
41
1

23
31
16
-
17
-






(Current
Phosphorus
Loading ) /
(Permissible
Phosphorus
Loading )

45
5

24
19
20

18
16
15
39
43
1

27
38
13
14
6
29






                            357

-------
      Table 40 (continued).  RANKING OF US OECD WATER BODIES
               USING SECCHI DEPTH,  CHLOROPHYLL a, EXCESS
               CHLOROPHYLL AND EXCESS  PHOSPHORUS LOADING AS
               RANKING PARAMETERS
EXPLANATION (continued)

 Based on mean summer values.

 Based on samples taken from upper two meters of water column

 Based on summer surface values.

6Based on August values from 1970 to 1974.

 Based on euphotic zone measurements.

^Based on mean of summer and winter concentrations.

 Dash (-) indicates data not available.
                                  358

-------
                               Table 41 .  RELATIVE TROPHIC STATUS  RANKING  OF  US  OECD WATER
                                          BODIES USING SECCHI DEPTH,  CHLOROPHYLL a,  EXCESS
                                          CHLOROPHYLL a AND EXCESS  PHOSPHORUS LOAD
GO
en
CD
Mean Secchi
Depth
(m)
Tahoe
Waldo
George
Washington -
197"+

Blackhawk
Washington -
1971
Sammamish
Meander1 —
1971
Meander -
1972

Mendota
West Twin -
1973
Dogfish -
1971
Dogfish -
1972
Harriet
Mean Chlorophyll
(yg/D
Tahoe
Waldo
Meander - 1972
Michigan -
Open Waters -
1971

Lamb - 1972
Harriet
Dogfish - 1972
Cayuga - 1973

Meander - 1971
Michigan
Nearshore
Waters - 1971
Brownie
Calhoun
Cayuga - 1972
Dogfish - 1971
Lamb - 1971
Washington -
1971
(Current Chlorophyll a)/
(Permissible Chlorophyll
Tahoe
Waldo
Meander - 1972
Dogfish - 1972
Michigan
Open Waters - 1971
(T = 30 S 100 yrs)
10
rjclir*!1 J- &L
Lamb - 1972
Meander - 1971

Dogfish - 1971
Lamb - 1971
Dogfish - 1971
Sammamish
Cayuga - 1973
Brownie
Calhoun
Washington - 1971
Weir
Camelot -Sherwood
(Current Phosphorus Load)/
a) (Permissible Phosphorus Load)
Waldo
Dogfish - 1972
Lamb - 1972
Meander - 1972
Tahoe

George
Weir
Michigan
Open Waters -
(T^ = 30 yrs)

Michigan
Open Waters -
(T = 100 yrs)
CO








1974


1974

Minnetonka - 1973
Michigan
Open Waters -
(T = 30 yrs)
Michigan
Open Waters -
(T = 100 yrs)
1971


1971

Washington - 1971

-------
                   Table 41 (continued).
RELATIVE TROPHIC STATUS RANKING OF US OECD WATER
BODIES USING SECCHI DEPTH, CHLOROPHYLL a, EXCESS
CHLOROPHYLL a AND EXCESS PHOSPHORUS LOAD
CO
CD
O
Mean Secchi
Depth
(m)
Cayuga - 1972
Cayuga - 1973
Michigan
Open Waters -
1971
Shagawa
East Twin -
1973
West Twin -
1974
Lamb - 1972

West Twin -
1972
Washington -
1957
Calhoun
Camelot-
Sherwood
East Twin -
1974

Weir
Canadarago -
1969

Mean Chlorophyll
(yg/i)
Came lot -Sherwood
Canadarago - 1969
Weir
Mendota
Lower Minnetonka
1973
Washington -
1957
Stewart
Canadarago - 1968
Redstone

Kerr-Roanoke Arm
Blackhawk
Potomac -Lower
Reach
Shagawa
Twin Valley
Cedar
Washington-1964
Lower Minnetonka
1969
Kerr-Roanoke Arm
(Current Chlorophyll a)/
(Permissible Chlorophyll a)
Canadarago - 1968
Cayuga - 1972
Minnetonka - 1973
Canadarago - 1969
Washington - 1957
Stewart
Redstone
Kerr Reservoir
-Roanoke Arm
Blackhawk

Kerr Reservoir
-Nutbush Arm
Potomac Estuary
-Lower Reach
Kerr Reservoir
-Nutbush Arm
Twin Valley
Cedar
Mendota
Minnetonka - 1969
East Twin - 1973
Shagawa
(Current Phosphorus Load)/
(Permissible Phosphorus Load)
Washington - 1974
West Twin - 1974
West Twin - 1973
Cedar
West Twin - 1972
East Twin - 1973
Canadarago
Sammamish
East Twin - 1974
Shagawa

East Twin - 1972
Minnetonka - 1969
Cayuga
Washington - 1957
Harriet
Wingra
Kerr Reservoir -
Nutbush Arm
Potomac Estuary -
Lower Reach
Calhoun

-------
                       Table  41  (continued).
RELATIVE TROPHIC STATUS RANKING OF US OECD WATER
BODIES USING SECCHI DEPTH, CHLOROPHYLL a, EXCESS
CHLOROPHYLL a AND EXCESS PHOSPHORUS LOAD
CO
CD

Mean Secchi
Depth
(m)
Cedar
Lamb - 1971
Lower Minne-
tonka - 1973
Redstone











Mean Chlorophyll
a (Current Chlorophyll a)/
(yg/1) (Permissible Chlorophyll
East Twin - 1973 West Twin - 1973
East Twin - 1972
Cox Hollow
East Twin - 1974
West Twin - 1974
Virginia
Dutch Hollow
West Twin - 1972

Isles
Potomac Estuary -
Middle Reach
Potomac Estuary -
Upper Reach


(Current Phosphorus Load)/
a) (Permissible Phosphorus Load)
Came lot -Sherwood
Dutch Hollow
Kerr Reservoir -
Roanoke Arm
Brownie
Mendota
Washington - 1964
Twin Valley
Redstone

Blackhawk
Cox Hollow
Virginia
Isles
Stewart
Sallie
                                                                         Potomac  Estuary -
                                                                           Middle Reach
                                                                         Potomac  Estuary -
                                                                           Upper  Reach

-------
     A plot of the ratio of the current phosphorus loading/per-
missible phosphorus loading and the mean chlorophyll a concentra-
tions for the US OECD water bodies is presented in Figure 86.
This correlation was developed to relate the excess phosphorus
loading of a water body (as related to its permissible phosphorus
loading) to water quality parameters.  Lines corresponding to
Vollenweider's permissible and excessive loadings (Figure 19)  can
be inserted in Figure 86.  The permissible line corresponds to a
current phosphorus load/permissible phosphorus load quotient of
one (i.e., the current and permissible phosphorus loads are
identical) while a quotient of two (i.e., the current phosphorus
load is twice the permissible phosphorus load) denotes the
excessive loading level on the Vollenweider diagram.  Figure 86
indicates a reasonably good agreement between the investigator-
indicated trophic conditions and the predicted trophic conditions
based on this relationship.  There are apparent anomalies and
data scatter which may be due to possible errors in the estimates
of either the phosphorus load or mean chlorophyll a_ values, as
well as a number of other factors.  For example, the possibility
of underestimations of the phosphorus loads for Lakes Dogfish,
Lamb and Meander was addressed earlier.  The situation with respect
to the lag time between a phosphorus loading reduction and a new
steady state chlorophyll a concentration for Lakes Washington and
Minnetonka have also been addressed.  Lake Weir, possibly because
of its subtropical nature relative to the northern US temperate
conditions of the other US OECD water bodies, also exhibits an
anomalous fit.
In general, however, there is a relationship between the current
phosphorus load/permissible phosphorus load quotients and the
resultant summer chlorophyll a concentrations for the US OECD
water bodies.   The agreement lends support to the use of this
approach for assessing the trophic conditions of water bodies,
based on their excess phosphorus ^loadings above a permissible
level, and the resultant chlorophyll a concentrations in the
water bodies.

     A plot was also made of the ratio of the current phosphorus
load to the permissible phosphorus load and the ratio of the
current chlorophyll a concentration to the permissible chlorophyll
a. concentration (Figure 87).  As indicated earlier, the permissible
chlorophyll a concentration (i.e., 2 yg/D was the summer mean
concentration corresponding to Vollenweider's permissible phos-
phorus loading line (Figure 19).  One can view this graph as a
correlation between the "excess" phosphorus loading, as expressed
in the current load/permissible load quotient, and the "excess"
summer chlorophyll a concentration, as expressed in the current
chlorophyll a concentration/summer permissible chlorophyll a con-
centration (i.e., 2 yg/1) quotient.  There is a reasonably good
agreement between these two parameters.  If the water bodies
which have been accepted as anomalous on the basis of various
previous analyses (i.e., Lakes Weir, Dogfish, Lamb, Meander,
Minnetonka - 1973, Twin Lakes, etc.) are removed, there is a

                                362

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             CURRENT  PHOSPHORUS LOADING

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     Figure 86.  Relationship Between  Excessive  Phosphorus
                Loads and Chlorophyll a^ in US OECD Water
                Bodies
                           363

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      Figure  87.
             Relationship  Between  Excessive
             Phosphorus  Loads  and  Excessive
             Chlorophyll a_ in  US OECD Hater
             Bodies
                         364

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better fit of the data sets to a 1:1 relationship.  This figure sug-
gests that for a given increase in phosphorus loading to a water
body, one can expect a proportional increase in the chlorophyll a_
concentration.  It should be noted that the correlation is a reason-
ably good one even though the summer chlorophyll  a concentrations
were not available for all US OECD water bodies, in which case the
annual mean values were used in Figure 87.  If one accepts a 1:1
relationship between these two parameters, this approach represents
a good water quality management tool in that it illustrates that if
a water body is receiving three times its permissible phosphorus
loading, it can be expected to have a mean epilimnetic summer chloro-
phyll a_ of about 3 times the permissible level of 2 yg/1.  One can,
of course, also use the value of the current phosphorus load/per-
missible phosphorus load quotient as a trophic ranking system for
a wide range of water bodies.

     The permissible and excessive phosphorus loading lines on
the Vollenweider phosphorus loading diagram (Figure 19) have
been related to the water quality parameters of mean summer epi-
limnetic chlorophyll a_, mean Secchi depth, and hypolimnetic
oxygen depletion in Figure 88.  The basis for this approach was
presented earlier.  A sequence of increasing chlorophyll a_ con-
centrations has been inserted into Figure 88 to illustrate how
a variety of boundary loading conditions can be translated into
a water quality parameter on Vollenweider's loading diagram.
Thus, an individual can literally set his own boundary phosphorus
loading levels, as a function of the mean depth/hydraulic
residence time characteristics of a water body, based on his
own concepts of acceptable chlorophyll a_ levels during the summer
season.  Further, by use of Equation 39, which relates chlorophyll
a_ levels and Secchi depths in natural waters, one can also sub-
stitute expected Secchi depths as boundary conditions in Vollen-
weider's loading diagram.  Using Equation 39, the permissible
loading line (i.e., chlorophyll a_ concentration of 2 jjg/1)
corresponds to a Secchi depth of approximately 4.6 meters while
the excessive loading line (i.e., chlorophyll a concentration of
6 ng/1) corresponds to 2.7 meters.  Finally, with the use of
Equation 41, relating hypolimnetic oxygen depletion to Secchi
depth, the permissible and excessive loading lines correspond to
hypolimnetic oxygen depletion rates of 0.3 and 0.6 mg 02/m2/day.
These depletion rates can, in turn, be applied to a water body's
total hypolimnetic oxygen volume to assess the effects of the
phosphorus load on the hypolimnetic oxygen content.  These levels
are consistent with generally accepted limnological observations.

     Thus, this new relationship (Figure 88) appears to relate a
phosphorus loading level to the more readily appreciated water
quality parameters of chlorophyll a_ concentrations and Secchi
depth.  Obviously, it may also be used as a trophic ranking sys-
tem, based on a water body's predicted chlorophyll a concentra-
tions and/or Secchi depths.  It has the feature of relating

                                365

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    100
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                                                OLIGOTROPHIC
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        Figure  88.  Relationship  Between  Vollenweider Phosphorus
                   Loading Diagram,  Summer Mean Chlorophyll  a_
                   and Secchi  Depth.
1000
                                366

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Vollenweider's criteria to mean summer conditions in a water body.
As indicated earlier, the summer period is usually the period of
greatest recreational use of a water body.  Consequently, this
approach allows individuals concerned with water quality manage-
ment to predict the phosphorus loading reduction necessary to
achieve an "acceptable" summer recreational level of chlorophyll
a or transparency in a water body.  This can then be translated
into costs, using methods indicated in an earlier section, so
that the cost-effectiveness of a given eutrophication control
program can be evaluated.

     It was not possible to satisfactorily test this latter rela-
tionship because of lack of sufficient data for the mean summer
chlorophyll a concentrations in most of the US OECD water bodies.
Even with the" data supplied by Dillon and Rigler (1974a) and by
Jones and Bachmann (1976), there was still insufficient data for
rigorous testing purposes.  The data supplied by Dillon and
Rigler fit Figure 88 reasonably well, although essentially all
his data sets were from oligotrophic water bodies in southern
Ontario.  The data from Jones and Bachmann on 16 Iowa Lakes
(1976) produced a poor fit in Figure 88.  However, it was also
noted that the data presented by these latter investigators
produced poor agreement between predicted and measured chlorophyll
a concentrations, by + 100 percent in some cases.  Jones and
Bachmann supplemented their data with literature values for 143
lakes in the determination of their regression equation.  However,
this data was not presented in their report, and thus could not
be tested for its fit in Equation 51.  Consequently, the authors
of this report offer this model only as a theoretical contribution
at the present.  However, it has its basis in the same theory and
assumptions as does Vollenweider's input-output phosphorus mass
balance model (Vollenweider, 1975a; 1976a) and is related to
several good correlations between the mean phosphorus, chlorophyll
a and Secchi depth in natural waters.  This model will be further
tested as more data sets become available, and the results
reported at a later date.  It appears to offer promise as a
quantitative tool both for ranking water bodies on a relative
trophic scale and for relating phosphorus loads to several
readily-appreciated water quality parameters.

     In summary, the approaches developed in this study offer
methods for the trophic rankings of water bodies based on their
displacement from the permissible loading line on the Vollenweider
diagram (Figure 19) as related to their predicted summer chloro-
phyll a and/or Secchi depth characteristics.  In general, these
approaches appear to complement each other and produce relative-
ly similar results.
                                367

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                         SECTION XIII

                          DISCUSSION
     From an overall point of view, based on initial analysis of
the US OECD eutrophication study data, it appears that the ap-
proach originally developed by Vollenweider and subsequently
modified by him, as well as by Dillon and by Larsen and Mercier,
has considerable validity as a tool for estimating phosphorus
loadings, average phosphorus concentrations and the associated
overall degree of fertility for many US lakes and impoundments.
In general, based on the US OECD investigators' classifications
of the trophic states of their respective water bodies, the US
OECD water bodies can be classified into groups with similar
phosphorus loads and morphometric and hydrologic characteristics.
That is, lakes and impoundments which are generally recognized
as being eutrophic in character plot together in each of the
various loading-response relationships which have been investi-
gated in this study.  While the relationship among the water
bodies within a particular group change, depending on the parti-
cular relationship being used, the overall relative positions
of the water bodies hold reasonably well.

     This finding gives considerable validity to the nutrient
loading-water body fertility relationship approach originally
proposed by Vollenweider and recently adopted by the US EPA as
a basis for phosphorus loading water quality criteria (US EPA,
1975b; 1976a).  At this time, it appears that the phosphorus
loading.criteria presented in the US EPA Quality Criteria for
Water (US EPA, 1976a) should be modified to include some of the
recent modifications of Vollenweider, Dillon and Larsen and Mer-
cier.  These modifications are important for water bodies with
short hydraulic residence times, such as some impoundments.  From
the information available today, it appears that water bodies with
short hydraulic residence times may have a higher nutrient load-
ing without the same degree of excessive fertilization problems
as would be expected in water bodies with longer hydraulic resi-
dence times.  Conceptually, the nutrients are not present in
the water body for a sufficiently long period of time before
being flushed out so as to allow their utilization by the aquatic
plant populations .
                               368

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     One of the major difficulties that may be encountered in
attempting to utilize the US OECD results as a basis for develop-
ing uniform national nutrient loading criteria is the fact that,
except for one seepage lake in Florida and an impoundment in
North Carolina, all of the rest of the US OECD water bodies are
located in the colder climates of the East and West coasts and
the upper Midwest area of the US.  It is possible that nutrient
loading-response relationships for water bodies from "typically
cold" climates will not hold for the warmer climatic conditions
that prevail in the southeast and southwestern US.  Additional
studies should be conducted on warm water body nutrient—response
relationships to ascertain whether these relationships for cold
climates are also applicable for warm climates.

     Another factor which may play an important role in causing
southern water bodies to behave differently from their northern
counterparts is that many of these water bodies tend to be more
turbid because of suspended sediments resulting from erosion in
the watershed and suspension of sediment from the bottom (Lee,
1974b) .  Some Texas impoundments tend to have severe water quality
problems which are associated with floating macrophytes rather
than with the planktonic or attached algae typical of excessively
fertile waters in cold climates.  There is need for nutrient load-
response studies such as those currently being conducted as part
of the OECD international eutrophication study for water bodies
of this type.

     There are several aspects of the Vollenweider phosphorus
load-fertility response loading diagrams which should be dis-
cussed.  First, it is clear that the relatively simple model
originally developed by Vollenweider is a useful tool to formu-
late phosphorus load-response relationships in such a way as to
be useful as management tools for excessive fertilization con-
trol .   For the first time, those concerned with control of eutro-
phication have a basis for predicting the overall trophic state
of a particular water body and the associated water quality that
will arise from either an increase or decrease in its current
nutrient loadings.

     With respect to eutrophication modeling, Vollenweider has
demonstrated that nutrient loading, lake morphology (as mani-
fested in mean depth) and hydraulic residence time (i.e., "fill-
ing time") are the three primary factors which govern lake fer-
tility.  As further work is done with the Vollenweider loading
curves as part of the international OECD eutrophication study, it
is likely other factors (i.e., color, turbidity, seasonal flush-
ing and mixing regimes and temperature) will be found which will
further refine the Vollenweider loading relationships and thereby
explain apparent deviations from these relationships.

     There are certain conditions that must be met before the
Vollenweider loading diagram can be used in management of water

                               369

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quality.  The most important of these is that the diagram is only
valid as a predictive tool in eutrophication control when the pri-
mary control of excessive fertilization is through control of a
chemical element such as nitrogen or phosphorus ,  since the load-
ing diagram was developed for a limiting element.  It is not
technically valid to utilize a loading diagram based on phospho-
rus loads for eutrophication control when the key limiting ele-
ment is nitrogen.  This report has focused mainly on phosphorus
loading relationships.  This is justified on the  basis of the
water bodies that have been included in the US OECD eutrophica-
tion study.  The majority are phosphorus-limited  with respect to
algal growth requirements (Table 9).  However, there appear to
be large numbers of water bodies in the US which are nitrogen-
limited.  Yet, if phosphorus loading can be decreased to the ex-
tent that phosphorus becomes the limiting element in a water
body, then the use of VollenweiderTs phosphorus loading relation-
ship becomes valid again.  This report also discussed the results
obtained in the US OECD eutrophication study for  nitrogen load-
water body response relationships.  As discussed  in this report,
several techniques are available which can be used to assess
the key limiting nutrient in a water body.

     Another situation in which the Vollenweider  loading diagrams
may not be applicable is for water bodies with low light penetra-
tion.  As discussed above, there is reason to believe that water
bodies with high inorganic turbidity may behave anomalously, com-
pared to other US OECD water bodies, with respect to their nutri-
ent load-water body response relationships.  Piwoni and Lee (1975)
have noted a similar phenomenon for highly-colored waters and
lakes located in central Wisconsin.

     The Vollenweider loading curve may not be applicable with-
out modification to large impoundments with significant amounts
of stratified inter- or underflow which would cause nutrients
present in the inflow waters to not interact with, or be avail-
able to, aquatic plants located in the euphotic zone of the water
body.  Under these conditions, it may be necessary to modify the
loading relationship to utilize a modified hydraulic residence
time which would reflect the lack of mixing of the inflowing
waters with the euphotic zone waters.

     The Vollenweider loading diagram provides some useful infor-
mation on potential benefits to be derived from manipulating the
limiting nutrient input for a particular water body.  In general,
the log-log plot means that substantial reductions in the nu-
trient loads must be made before any significant  improvement in
water quality would be expected.   This was discussed in relation
to^the possible effects of a detergent phosphate  ban on eutro-
phication and water quality in a hypothetical water body in an
earlier section of this report.
                               370

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     The Vollenweider loading diagram allows a comparison to be
made of the general trophic status of a particular water body
relative to a certain nutrient loading.  While it is highly
successful in categorizing lakes and impoundments into groups
with similar trophic states and water quality, such a diagram
should not be used as a basis for classification of a water
body's trophic status.  One should not state that a lake has a
particular trophic state merely because of its position on the
Vollenweider loading diagram.  Rather, one can only indicate
that a water body of a given phosphorus loading and mean depth/
hydraulic residence time quotient tends to plot in the same re-
lative area of the Vollenweider diagram as water bodies of similar
phosphorus loads and mean depth/hydraulic residence time values.

     A logical extension of the Vollenweider loading diagram is
the development of a relationship between the position of a water
body on this curve, or a modification thereof, and the resultant
water quality in the water body in which concern is focused on
excessive fertilization problems.  Ultimately, it should be pos-
sible to make a quantitative estimate of the improvement in water
quality that may result in a water body from reduction of the nu-
trient loading by a certain amount.   The phosphorus load-chloro-
phyll a, Secchi depth and hypolimnetic oxygen depletion rate
relationships (Figures 22, 79 and 80, respectively) and the
Vollenweider phosphorus loading diagram incorporating boundary
conditions for chlorophyll a, Secchi depth and hypolimnetic
oxygen depletion (Figure 88T represent significant steps in this
direction.  Similar types of relationships should be explored
for various other types of nutrient load-water quality type re-
sponse parameters, such as domestic water supply tastes and odors,
shortening of water treatment plant filter runs, etc.

     Associated with  several of  the  load-response  relationships
discussed above are descriptive  terms  such as  "excessive",  "per-
missible",  "oligotrophic"  or "eutrophic" which  can  be  translated
into a  certain water  quality condition.   It  is  important to  em-
phasize that these narrative terms  go  back to  the  work  of  Sawyer
(19M-7)  who  established critical  nutrient concentrations for
approximately  20  south-central Wisconsin lakes.   Several indi-
viduals,  including Vollenweider  (1968) have  found  that  for many
lakes with  ice cover  during the  winter, Sawyer's  original  cri-
tical phosphorus  concentrations  can  be translated  into  water
quality deterioration which typically  manifests  itself  in  in-
creased "greenness" of the water.  The "greenness"  is  roughly re-
lated to  the chlorophyll  content of  the water.   Chlorophyll  a
values  of less than 5 yg/1 are considered to be  indicative of
oligotrophic waters with  high water  quality.   Chlorophyll  a  con-
centrations of greater than about 10 yg/1 are  often  associated
with waters classified as  eutrophic  and possessing  deteriorated
water quality  for many beneficial uses .  Chlorophyll concentra-
tions of  2  and 6  yg/1 were found for the Vollenweider  diagram
(Figure 88) permissible and excessive  loading  lines  in  an  earlier
section.
                               371

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     It is important to note, however, that Sawyer and others
have been involved with water bodies in which the primary problem
was generally the growth of excessive amounts of planktonic algae,
and in which this growth affected water clarity.  This is mani-
fested in an increased "greenness" during periods of algal blooms
and a decreased Secchi depth.  In addition, these water bodies
generally have planktonic algal growth limited by the phosphorus
content of water.  In general, these water bodies are natural
lakes with little or no color or turbidity.  During periods of
little or no algal growth, this water has a high degree of clarity
with Secchi depths exceeding 3 to 4 meters.  The residents of the
area who make use of those lakes which have 'excessive' loadings
find the water quality sufficiently impaired during periods of
algal blooms to curtail recreational use of these water bodies.

     The impairment of recreational use (i.e., boating, swimming
and fishing) has been used as the basis for determining what con-
stitutes 'excessive' loadings.  Lee (1974b) has discussed a pos-
sible lack of application of the Sawyer critical nutrient concen-
trations for the warmer water bodies of the southern US.  He noted
these critical concentrations may not produce the same deteriora-
tion of water quality in the normally turbid or colored waters
found in many southern US impoundments as would be expected in
water bodies in the north temperate zones of the US.  The public
does not perceive the same decrease in water clarity, resulting
from a certain magnitude of algal blooms, in normally turbid or
colored waters as would be perceived in a normally clear water
body.  Further, Lee (1974b) discussed the fact that in many parts
of the US the public will not perceive deteriorated water quality
to the same degree since all the water bodies in some areas of
the US normally have essentially the same water quality, in con-
trast to Wisconsin, Michigan and Minnesota, where there are
several thousand small lakes of widely varying water quality.
Therefore, it must be concluded that, without further study, one
cannot assume that  permissible' and 'excessive' loading criteria,
or for that matter, oligotrophic versus eutrophic waters, are
necessarily translated into the same degree of impairment of re-
creational use in various parts of the US.

     In addition to impairing recreational use of water, the
stimulation of algal growth by excessive nutrient loading may
also cause significant water quality deterioration in domestic
and industrial water supplies.  Lee (1971) has discussed the
potential effects of excessive fertilization on water supply
water quality.  The most significant problems are those of taste
and odor production associated with materials excreted from the
algae and a shortening of the length of filter runs.  The per-
missible and excessive criteria used on the various loading dia-
grams do not consider the potential effects of the nutrients on
water supply water quality.  From the point of view of eutro-
phication control in water supply water bodies, at least for cer-
tain types of algae, the excessive loading line in the Vollen-
weider and other phosphorus loading diagrams may have to be

                                372

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lowered significantly in order to minimize the problems of
excessive fertility on water quality in these water bodies .

     It is important to emphasize that the concepts of excessive
nutrient loading pertain to planktonic algal problems and do not
consider the problems of attached algae or attached or floating
macrophytes.  It is highly probable that the permissible and ex-
cessive nutrient loadings would also be different in those water
bodies which have a tendency to manifest their excessive nutrient
concentrations in the growth of nonplanktonic aquatic plants .

     Another aspect that should be considered with respect to
the Vollenweider loading diagram's emphasis on permissible and
excessive loadings based on recreational impairment is that  the
critical nutrient loading is the loading that impairs recreation-
al use.  For many water bodies, algal growth problems which  may
affect extensive recreational use of the water body are essen-
tially restricted to the summer months.  In general, from the
point of view of recreational use, there is little concern about
the algal blooms that occur in late fall in association with
fall overturn and the transport of hypolimnetic nutrients to the
surface waters.  Further, algal blooms under the ice, or just
after ice-out, are usually of little or no significance to im-
pairment of recreational use of the water body.  Therefore,  as
a potential modification of the Vollenweider loading diagram,
it is important to consider the nutrient transport to, and
cycling within, a water body in relation to how a particular
nutrient loading affects water quality.  There will likely be
situations where major nutrient loads added in late fall or
during the winter period will have little or no effect on the
following summer's planktonic algal growths.  This is an area
that needs additional study to determine the critical nutrient
loads that have the greatest impact on the water body's water
quality.

     Examination of the US OECD water bodies for correlations
between their nutrient loads and selected eutrophication re-
sponse parameters (Table 26) has been useful in some instances,
although not for all parameters.  A major problem which limits
the usefulness of many of the correlations is that standardiza-
tion of data was not possible in many cases.  Data for specific
parameters was scarce for many water bodies.  Further, as indi-
cated in Table 11, a variety of analytical procedures were used
to determine the various chemical, biological and physical
parameters of interest in the US OECD eutrophication study.
Also, a wide variety of sampling methodologies (Appendix II)
were employed by the various US OECD investigators.

     This lack of uniform analytical and sampling methodologies
was due in part to the nature of the US OECD eutrophication
study.   As indicated in an earlier section, essentially no new-
lake studies were begun in the US portion of the OECD

                               373

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Eutrophication Project.  Rather, in general, lakes which had
been studied extensively in the past were selected for inclusion
in the US OECD eutrophication study.  In many cases, the goals
of the previous studies on the US OECD water bodies were dif-
ferent from those of the overall OECD efforts.  This factor is
a cause of at least part of the problem of standardization of
data.  This lack of standardization has made direct comparability
of data between US OECD water bodies of limited value.  Inability
to compare eutrophication data between water bodies , as indi'-
cated by Vollenweider (1968), was a major impetus to initiation
of the current OECD Eutrophication Project.

     In general, the results of the correlations have indicated
the large majority of US OECD water bodies are phosphorus-limited
with respect to algal nutrient requirements.  The correlations
between the phosphorus loads and the various eutrophication re-
sponse parameters are usually better than those between nitrogen
loads and the same response parameters.  The exception is the
relationship between phosphorus loads and both annual and grow-
ing season dissolved phosphorus in the US OECD water bodies,
which shows essentially no correlation.  By contrast, there is
a good correlation between nitrogen loads and inorganic nitrogen,
indicating that the inorganic nitrogen is not being used by the
algal populations in proportion to its supply to most of the US
OECD water bodies.  There is essentially no correlation between
either dissolved phosphorus or inorganic nitrogen and mean
chlorophyll a.  By contrast, a good correlation is seen between
total phosphorus and mean chlorophyll a supporting the importance
of phosphorus in controlling algal growths in most of the US OECD
water bodies.  This is consistent with the observations concern-
ing algal-limiting nutrients reported by the US OECD investi-
gators (see Table 9).

     It is likely that many of the apparently good correlations
observed between nitrogen loadings-concentrations and eutrophica-
tion response parameters are coincidental artifacts of the rela-
tively constant N:P loading ratio observed in the US OECD water
bodies (see Figures 19 and 21).  This was noted earlier by
Vollenweider (1968), although he used a. slightly higher N:P
loading ratio (i.e., 15N:1P (by weight)) in the derivation of
his nitrogen loading and mean depth relationship (see Figure 6)
than was indicated in Figures 19 and 21 in this report.

     Several of these correlations were useful in the derivation
of several of the relationships derived to evaluate expected
changes in water quality resulting from changes in nutrient loads
to the US OECD water bodies.  Particularly, the relationship be-
tween the phosphorus loads and chlorophyll a_, between chlorophyll
a_ and Secchi depth, between hypolimnetic oxygen depletion rate and
Secchi depth, and between spring overturn total phosphorus
and  summer chlorophyll a served as the basis for most of these
water quality models  (see Figures 22, 78 and 79).  These

                               37H

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 relationships  have  been  observed  in many  other water  bodies ,  in
 addition to  the  US  OECD  water  bodies,  substantiating  their oc-
 currence in  water bodies of  differing  trophic conditions.  Using
 the  water quality model  relationships  derived in  this report,
 it  is  now possible  to  make a technically  sound evaluation of  the
 effects  of any given water quality management program.  In the
 past,  eutrophication control programs  have  largely  been directed
 toward the removal  of  phosphorus  from  domestic wastewater sources.
 However, this  approach has been largely subjective.   The water
 quality models derived in this report  offer practical tools for
 individuals  concerned  with water  quality  management and eutro-
 phication control.

     These water quality models have several advantages over
 previous eutrophication  modeling  efforts.   First , they are re-
 lated  to common  eutrophication response parameters  which are
 readily discernible to both  scientist, engineer and layman.
 While  the Vollenweider loading diagram (Figure 19)  offers a good
 indication of  the overall eutrophication  of the US  OECD water
 bodies,  these  water quality  models then relate the  relative de-
 gree of fertility of the US  OECD  water bodies into  three common
 eutrophication response  parameters, namely  chlorophyll a con-
centrations, Secchi depth, and the hypolimnetic oxygen depletion
rate.  These first two parameters, both related to the "green-
ness"  or transparency of water bodies,  are more widely appre-
ciated and understood as a good overall indicator of water
quality  that the public  could perceive than would be the know-
ledge  concerning the extent  of areal total phosphorus loading
reduction necessary to achieve a permissible phosphorus load.
Another  feature of these models is that they are simple,  re-
quiring  only knowledge of easily-measured parameters.   They are
also based on  observations concerning nutrient load-eutrophication
response relationships which have been observed in a wide range
of water bodies,  lending credibility to their general applica-
bility.

     One of  their main features is that they allow evaluation of
the  effects  of a phosphorus  eutrophication  control program prior
to initiation  of the program.  This information will  enable water
quality  managers to inform the public of the expected  increase
in water quality that  can be achieved as a  result of  controlling
phosphorus from  each of  the  potentially available sources for a
particular water body  to a selected degree.  A proper  cost-
benefit  analysis can then be conducted for  a given eutrophication
control  program prior  to its initiation.  With this knowledge and
the water  quality models derived in this report, the  public can
then determine whether the expected results  of a given eutro-
phication  control program are  justified by  its expense.  Lee
(1976)  has used these above approaches  in evaluating the  ex-
pected water quality benefits to be derived for the Great
Lakes from a phosphate-built detergent  ban in the State of
Michigan.
                               375

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     The Vollenweider phosphorus loading diagram has been re-
lated to the same water quality parameters (see Figure 88).  This
relationship was derived in an earlier section of this report
(i.e., Equations 40, 47 and 48).  Vollenweider's later models
(1976a), as well as those of Dillon (1975) and Larsen and Mer-
cier (1976) have their basis in the same theoretical phosphorus
mass balance approach as was used to derive the Vollenweider
phosphorus loading diagram (Figure 19).  Consequently, relating
the Vollenweider phosphorus loading diagram to these water quality
parameters is pleasing in that it relates the above-mentioned
models of Vollenweider, Dillon and Larsen and Mercier to these
same parameters.  Relating them to more readily appreciated
water quality parameters will likely enhance their application
as eutrophication evaluation methodologies.

     The results of the trophic status index study indicated
that, in general, the trophic classification systems of the US
EPA (1974d), Carlson (1974) and Piwoni and Lee (1975) produce
approximately the same relative trophic rankings for the US OECD
water bodies.  There are a few anomalies noted with all three
indices, with some water bodies of more fertile conditions ranked
more toward the oligotrophic end of the trophic spectrum than
less fertile water bodies.  All three ranking systems producing
similar results may be partially due to the fact that all three
systems have several common parameters.  These parameters may
have been of sufficient importance in the trophic rankings,
relative to the other parameters, that they influenced all three
systems toward similar results.

     The approach developed in this report of ranking the US OECD
water bodies on the basis of their excess phosphorus loads (i.e.,
ratio of current phosphorus load to permissible phosphorus load)
offers another simple method of relating phosphorus loads to
eutrophication response parameters.  Examination of the rela-
tionship between the excess phosphorus load and mean chlorophyll
a (Figure 86) shows a positive correlation exists between these
parameters, although there is a scatter of the data.  This data
scatter is due in part to the fact that the mean chlorophyll a
values used in this relationship are a mixture of annual and
growing season values.  This relationship is similar to that of
Vollenweider which relates the phosphorus load, as modified by
mean depth and hydraulic residence time, to the mean chlorophyll
a (see Figure 22), except that the chlorophyll a is being corre-
lated with the excess phosphorus load in this model.

     The relationship between excess chlorophyll a_ and excess
phosphorus' load also showed good promise as a water body trophic
ranking system.  The excess chlorophyll a was referenced to the
permissible 2 yg/1 chlorophyll a concentration derived in an
earlier section (see Equations 4~8 and 49).  This relationship
(Figure 87) is interesting in that, although the data is somewhat


                                376

-------
scattered, it appears to illustrate a 1:1 relationship between
the excess phosphorus load and excess chlorophyll a in the US
OECD water bodies.  This suggests that a water body receiving
a phosphorus load of a certain magnitude above the permissible
level will experience a mean summer chlorophyll a_ level of
essentially the same proportion above the 'permissible' chloro-
phyll level.  While this is not unexpected to some degree, it is
surprising to note that this approximately 1:1 relationship
between excess phosphorus and excess chlorophyll a_ appeared to
hold over the whole phosphorus loading and chlorophyll a_ range
of the US OECD water bodies.  Thus, one could use the current
phosphorus load/permissible phosphorus load quotient as a trophic
ranking system for a wide range of water bodies.

     The applicability of the Vollenweider loading relationships
for shallow lakes is an area that needs further attention.
Examination of the US OECD eutrophication study data, although
limited for these types of water bodies , shows that shallow
lakes and impoundments do not appear to have significantly
different chlorophyll a and Secchi depth responses to phosphorus
loads than do the other US OECD water bodies (Figures 22 and 79,
respectively).   It should be noted that the nutrient load estimates
for many of the shallow lakes and impoundments are based on land
use in the watershed and the appropriate nutrient export coeffic-
ients .   Because of the uncertainty of the nutrient loads for these
water bodies at this time, it would be inappropriate to conclude
that shallow water bodies have different nutrient load-eutrophica-
tion response relationships than do deeper water bodies.

     The primary distinguishing feature between shallow lakes and
deeper lakes is the absence of thermal stratification.  For the
purposes of this report, a shallow lake is one with a mean depth
of 3 m or less.  Generally, water bodies of this type do not
thermally stratify, except under highly sheltered conditions in
which wind-induced mixing of the water column is  hampered.  The
lack of permanent thermal stratification during the growing
season  plays a  major role in nutrient recycling.   In deep lakes
(i.e.,  lakes that remain thermally-stratified during the entire
growing season), the thermocline represents  a barrier to nutrient
recycling from the hypolimnetic waters.   The effectiveness of the
thermocline as  a nutrient barrier is highly variable and varies
from lake to lake.  As discussed by Stauffer and Lee (1973),
some water bodies, such as Lake Mendota in Madison, Wisconsin,
which permanently stratify during the summer, still derive
appreciable nutrients from the hypolimnion,  as a result of thermo-
cline migration.  In fact, this phenomenon appears to be the pri-
mary controlling mechanism governing many of the  algal blooms
that occur in Lake Mendota during the summer.

     As shown by Lee et. a_l (1976), appreciable phosphorus re-
cycling occurs  in aerobic waters.  This recycling is associated

                                377

-------
primarily with mineralization of algal phosphorus.   This phenomenon
would be especially important in shallow lakes because they tend
to have warmer water overall than the surface waters of deeper
lakes of the same region.   The higher temperatures  in the shallow
lakes would promote the phosphorus mineralization.   This higher
overall temperature, in addition to increasing the  rate of
nutrient recycling, also affects many other factors controlling
algal growth including the algal growth rate and algal predation
by zooplankton.  Further,  higher temperatures would likely have
some effects on the types  of algae present.  It is  therefore
reasonable to conclude that, as a result of their somewhat
elevated temperatures compared to deeper water bodies , shallow
lakes would tend to use their nutrients , especially phosphorus ,
to a somewhat greater degree and at a faster rate.   This could cause
shallow lakes to not fit as well as deeper water bodies in the
Vollenweider nutrient load-eutrophication response  relationships
or to deviate from the Vollenweider nutrient load relationships
which were developed for deeper water bodies.

     Another factor which  could influence the behavior of shallow
lakes,  compared to deeper  water bodies , in the Vollenweider
phosphorus loading relationships is water clarity.   In general,
shallow water bodies tend  to be more turbid as a result of suspen-
sion of the sediments into the water column.  This  suspension arises
from several factors, the  most important of which is wind-induced
mixing.  Also important in their suspension is the  mixing of sedi-
ments to the overlying waters from the activities of fish, such
as carp burrowing in the sediments.  As discussed by Lee (1970),
anaerobic fermentation of the sediments, as well as benthic organ-
ism biomass suspension due to photosynthesis, also  contribute to
the mixing of the sediments in the water column.  Another factor
which would tend to make shallow lakes more turbid  in hardwater
areas is the precipitation of calcium carbonate which, under cer-
tain extreme conditions, can produce a "milky" appearance in the
water column.

     The elevated turbidity often present in shallow lakes could
cause these water bodies to deviate from the Vollenweider relation-
ships in a variety of ways.  One of the most important of these
possible deviations is the promotion of light limitation of algal
growth.  Therefore, even though water temperatures  would tend to
be higher and aerobic nutrient recycling faster in  shallow lakes,
algal growth in these water bodies may not be stimulated because
of increased detrital and  mineral activity in the water, which
could cause a light limitation of algal growth in these water bodies

     This increase in nonalgal turbidity in shallow lakes would
tend to make phosphorus somewhat less available for algal growth
because of sorption and precipitation reactions in the water body.
Detrital minerals, especially clays, have a relatively high capac-
ity for phosphate uptale.   Also, calcium carbonate  precipitation
in hard water systems would probably result in coprecipitation of

                                378

-------
hydroxyapatites.  On the other hand, since the water in shallow
lakes is almost always oxygenated, phosphate sorption by freshly
precipitated iron hydroxide would be minimal,  Thus , from an over-
all point of view, it is likely that less of the phosphorus
added to a shallow lake would be available to promote algal
growth than would be seen  in  deeper water bodies.

     The increased turbidity often present in shallow lakes would
tend to greatly alter the public's response to planktonic algal
growths.  The public in general tends to perceive change in a
water body as a significant detrimental factor,  Planktonic
algal growth in a water body that is generally somewhat turbid
because of sediment suspension in the water column would be less
objectionable to the public since the effect of the algal on
overall water clarity is more difficult to perceive than in less
turbid water bodies ,  In a study currently being conducted by
Lee et_ al. (1977), it has been found that Lake Ray Hubbard, an
impoundment near Dallas, Texas, tends to have a markedly different
chlorophyll-Secchi depth relationship than do the US OECD water
bodies.  Several arms of this impoundment are 1 to 3 m deep and
contain large amounts of mineral and detrital turbidity in the
water column.  A given planktonic algal chlorophyll in this lake
is associated with a significantly shallower Secchi depth than
found in typical US OECD eutrophication study water bodies.
Large algal blooms occur in this lake, yet have limited impact
on its recreational use because the planktonic chloroDhyll does
not change overall water clarity to a significant degree compared
to non-bloom conditions in the water body.

     Many shallow lakes and the shallow waters of deeper lakes tend
to support large populations of attached algae and macrophytes.
Since the Vollenweider nutrient relationships are based primarily
on planktonic algal chlorophyll, growth of non-planktonic plants
tend to act as a sink for nutrients during the growing season.
Therefore, less planktonic algal production will occur in shallow
lakes containing high populations of attached algae and macrophytes

     From the above discussion it is apparent that a variety of
factors would tend to cause shallow lakes to deviate from the
Vollenweider nutrient load-eutrophication response relationships.
However, the effects of many of these factors tend to oppose one
another, with the result that it is impossible at this time to
predict, without additional study, whether shallow lakes and
impoundments will tend to show different nutrient load-eutrophica-
tion response relationships than other deeper water bodies .  The
combined OECD Eutrophication Program study data from the Alpine ,
Nordic, North American and Shallow Lakes and Impoundments Projects
will likely provide a sufficient data base to determine whether
shallow lakes and impoundments tend to deviate significantly from
the nutrient load-eutrophication response relationships than
deeper water bodies.
                                379

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     Drainage Area Characteristics and Non-Point Nutrients in
     Streams.  National Eutrophication Survey Working Paper No.
     25, Pacific Northwest Environmental Research Laboratory,
     Corvallis.   50 pp.

US Environmental Protection Agency.  1974d.  An Approach to a
     Relative Trophic Index System for Classifying Lakes and
     Impoundments.  National Eutrophication Survey Working Paper
     No. 24, Pacific Northwest Environmental Research Laboratory,
     Corvallis.   44 pp.

US Environmental Protection Agency.  1975a.  National Eutrophica-
     tion Survey Methods, 1973-1976.  National Eutrophication
     Survey Working Paper No. 175, Pacific Northwest Environ-
     mental Research Laboratory, Corvallis.  91 pp.

US Environmental Protection Agency.  1975b.  US EPA Quality
     Criteria for Water.  (Draft released in November, 1975,
     for public  comment.  Washington, D.C.)  Document undated.
     501 pp.

US Environmental Protection Agency.  1975c.  National Water
     Quality Inventory.  1975 Report to Congress.  Office of
     Water Planning and Standards, Washington, D.C.  48 pp.

US Environmental Protection Agency.  1976a.  Quality Criteria
     for Water.   US EPA Report EPA-440/9-76-023.  501 pp.

US Environmental Protection Agency.  1976b.  Water Quality
     Criteria Research of the US Environmental Protection
     Agency.  Proceedings of an EPA-Sponsored Symposium on
     Marine, Estaurine and Fresh Water Quality.  US EPA Report
     EPA-600/3-76-079.  pp. 185-205.

Uttormark, P. D., J. D. Chapin and K. M. Green.  1974.
     Estimating  Nutrient Loading of Lakes from Non-Point
     Sources.  US EPA Report EPA-66 D/3-74-02'0 .  112 pp.

Vallentyne, J. R.  1974.  The Algal Bowl.  Information Canada,
     Special Publication No. 2"2 , Department of the Environment,
     Fisheries and Marine Service, Ottawa, Ontario.  186 pp.

Vollenweider, R, A.  1968.  Scientific Fundamentals of the
     Eutrophication of Lakes and Flowing Waters, with Particular
     Reference to Nitrogen and Phosphorus As Factors in Euto-
     phication.   Technical Report  DAS/CSI/68.27,  Organization for
     Economic Cooperation and Development (OECD), Paris.   250 pp.


                                396

-------
Vollenweider, R. A.  1969.  Moglichkeiten Und Grenzen elementarer
     Modelle der Stoffbilanz von Seen. Arch. Hydrobiol. 66:l-36.

Vollenweider, R, A.  1975a.  Input-Output Models, with Special
     Reference to the Phosphorus Loading Concept in Limnology.
     Schweiz Z. Hydrol .  3_7 : 53-84,

Vollenweider, R. A,  1975b.  Personal Communication.  (Canada
     Centre for Inland Waters-Burlington, Ontario).  June  13,
     1975 .

Vollenweider, R, A.  1975c.  Personal Communication.  (Canada
     Centre for Inland Waters-Burlington, Ontario).  August 15,
     1975.

Vollenweider, R. A.  1975d.  Personal Communication.  (Canada
     Centre for Inland Waters-Burlington, Ontario).  December 8,
     1975 .

Vollenweider, R. A.  1976a.  Advances in Defining Critical
     Loading Levels for Phosphorus in Lake Eutrophication.  Mem.
     1st. Ital. Idrobio. 3_3_: 53-83.

Vollenweider, R. A.  1976b.  Personal Communication.  (Canada
     Centre for Inland Waters-Burlington, Ontario).  January 13,
     1976 .

Vollenweider, R. A.  1977.  Personal Communication.  (Canada
     Centre for Inland Waters-Burlington, Ontario).  January 20,
     1977 .

Vollenweider, R. A. and P. J. Dillon.  1974.  The Application
     of the Phosphorus Loading Concept to Eutrophication Research,
     National Research Council Canada, NRC Associate Committee
     on Scientific Criteria for Environmental Quality, NRCC No.
     13690.  42 pp.

Weber, C. A.  1907.  Aufblau und Vegetation der Moore
     Norddeutschlands,   Beiblatt zu den Botanischen Jahrbuchern
     9£:19-34.

Weibel, S.  R.  1969.  Urban Drainage as a Factor in Eutrophica-
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     Correctives , Proc .  SyrnT^ N"aTiwTaT~Academy of ScTeTnce ,
     Wa shingtorTV D . C .   pp. 383-403.


Weiss, C.M.  1977.  Personal Communication.  (Department  of
     Environmental Science and Engineering, University of
     North Carolina, Chapel Hill)  June 16, 1977.


                                 397

-------
Weiss, C. M.  and J.  H.  Moore.   1975.  The John H. Kerr
     Reservoir, Virginia-North Carolina.  A Report for the
     OECD North American Project Defining its Limnological
     Characteristics, Productivity, Nutrient Budgets and
     Associated Parameters.   Report to US EPA, Environmental
     Research Laboratory, Corvallis.  38 pp.

Welch, E. B. , T. Wiederholm, D. E. Spyrdakis and C. A. Rock.
     1975.  Nutrient Loading and Trophic State of Lake
     Sammamish, Washington.   Report to US EPA, Environmental
     Research Laboratory, Corvallis.  21 pp.

Welch, E. B.  and M.  A.  Perkins.  1977.  Oxygen Deficit Rate as
     a Trophic State Index.   Draft Manuscript, Department of
     Civil Engineering, University of Washington, Seattle.
     12 pp.

Wiebe, J. D.  (ed.).   1973.  Water Quality and Land Use Activities
     Proceedings of a IJC Workshop Held at Univ. of Guelph,
     Ontario, September 11-12, 1973.  248 pp.

Wright, R. F.  1974.  Forest Fire:  Impact on the Hydrology,
     Chemistry and Sediments of Small Lakes in Northeastern
     Minnesota.  Interim Report No. 10, Limnological Research
     Center,  University of Minnesota, Minneapolis.
                                 398

-------
                         APPENDIX I

                    FINAL REPORT OUTLINE
                  (North American Project)

  I.  Introduction - Short Past History of Water Body
 II.  Brief Geographical Description of Water Body
      A.   Latitude and Longitude (Centroid of Water Body)
      B.   Altitude Above or Below Sea Level
      C.   Catchment Area (Including Area of Surface Water)
      D.   General Climatic Data (Ice Coverage; Average Month-
          ly Air Temperature; Wind Patterns; Evaporation; etc.)
      E.   General Geological Characteristics (Nature of
          Bedrock; Subsoil and Soils; Importance of Land
          Erosion)
      F.   Vegetation
      G.   Population
      H.   Land Usage (Industrial, Urban, Agricultural, etc.)
      I.   Use of Water (Drinking, Sport, Fishing, etc.)
      J.   Wastewater Discharges (Population and Industry)
III.  Brief Description of Morphometric and Hydrologic Char-
      acteristics of Water Body
      A.   Surface Area of Water (Length, Width, Shore Length,
          etc. )
      B.   Volume of Water (Information on Regulation)
      C.   Maximum and Mean Depth
      D.   Ratio of Epilimnion over Hypolimnion
      E.   Duration of Stratification
      F.   Nature of Lake Sediments
      G.   Seasonal Variation of Monthly Precipitation
          (Maximum, Minimum Conditions on Drainage Basin)
                              HOO

-------
    H.   Inflow and Outflow of Water (Also Underground)
    I.   Water Currents
    J.   Water Renewal Time  (Residence Time)
IV.  Limnological Characterization Summary
    A.   Physical
        1.   Temperature
        2.   Conductivity
        3.   Light
        4.   Color
        5.   Solar Radiation
    B.   Chemical
        1.   pH
        2.   Dissolved Oxygen
        3.   Total Phosphorus (Including Fraction Forms)
        4.   Total Nitrogen (Including Fraction Forms)
        5.   Alkalinity arid/or Acidity
        6.   Ca, Mg, Na, K, SO^, Fe
    C.   Biological
        1.   Phytoplankton (Chlorophyll; Primary Productivity;
            Algal Assays; Identification and Count)
        2.   Zooplankton (Identification and Count)
        3.   Bottom Fauna
        4.   Fish
        5.   Bacteria
        6.   Bottom Flora
        7.   Macrophytes
 V.   Nutrient Budgets Summary
     A.   Phosphorus             Source          Kg/Yr
                          Waste Discharges       xx
                          Land Runoff            xx
                          Precipitation          xx
                          Ground Water           xx
                          Other                  xx
                                      Total      xx
                           401

-------
      B.   Nitrogen             _  Source            Kg/Yr

                           Waste Discharges          xx
                           Land Runoff               xx
                           Precipitation             xx
                           Ground Water              xx
                           Other                     xx
                                           Total     xx
      C.   Other Nutrient Budgets, If Available

 VI.   Discussion
      A.   Limnological Characterization

      B.   Delineation of Trophic Status
      C.   Trophic Status Versus Nutrient Budgets

          1.   Present Vollenweider Numbers

              (Grams/Meter2/Year)
          2.   Mean Depth/Hydraulic Residence Time

VII.   Summary
                             402

-------
                         BLACKHAWK (WISC. ) """
          DATA SUMMARY  FOR US  OECD EUTROPHICATION  PROJECT
                           (IMPOUNDMENT)
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depthb
Mean Dissolved Phosphorus13
Mean Total Phosphorus
Mean Inorganic Nitrogen
 (NHJ+N03+N02 as N)
Mean Chlorophyll a
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Summer Mean Epilimnetic Values:
   Total Phosphorus
   Dissolved Phosphorus
   Inorganic Nitrogen
Eutrophic in 1972-1973
3.6 x 107 m2
8.9 x 105 m2
4.9 m
0 .5 yr
227 mg/1 as CaC03
471 ymhos/cm @ 25°C
3.6 m
0.04° mg P/l
0.12C mg P/l
1.02  mg N/l
14.6 yg/1 (first two meters of
           water column)
0 kg P/yr
1900-2070 kg P/yr
2.13-2.32 g P/m2/yr

0 kg P/yr
20 ,900 kg N/yr
23.4 g N/m2/yr
0.05 mg P/l
0.015 mg P/l
0.54 mg N/l
                 Invest igator - Indicated Comments
 aDoes not  include water body surface area.
  Data based on  samples obtained at six-week intervals at either
  one or two meter depth intervals in the deepest part of the im-
  poundment .
 c         .
  Average winter concentrations.
 Dash  (-) indicates no data available.
*Data taken from Piwoni and Lee (1975)' and personal communication
  (Table 3).
                                404

-------
                           BROWNIE  (MINN.)
          DATA SUMMARY  FOR US  OECD EUTROPHICATION  PROJECT
                               (LAKE)
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus
Mean Total Phosphorus
Mean Inorganic Nitrogen
          as N)
Mean Chlorophyll a
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Eutrophic in 1971
           2
           2
4.7 x 105 m2
7.3x10  m
6.8m
2 .0 yr
123-136 mg/1 as CaCO,
                    O
400-475 ymhos/cm @ 25°C
1.5 m
< 0.01b mg P/l
< 0.055b mg N/l
5.9b yg/1


82.1C kg P/yr
3.8  kg P/yr
1.18 g P/m2/yr
               Investigator-Indicated Comments
 Does not include water body surface area.
 Summer average surface values
 Includes urban storm water drainage.
Dash (-) indicates no data available.
"Data taken from Shapiro (1975a) and personal communication
 (Table 3).
                                 405

-------
                           CALHOUN (MINN.)
          DATA  SUMMARY FOR US OECD EUTROPHICATION PROJECT
                               (LAKE)
Trophic  State
Drainage  Area
Water  Body  Surface  Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus
Mean Total  Phosphorus
Mean Inorganic Nitrogen
          as N)
 Mean  Chlorophyll  a_
 Annual  Primary  Productivity
 Phosphorus  Loading :
    Point  Source
    Non-Point  Source
    Surface  Area Loading
 Nitrogen  Loading:
    Point  Source
    Non-Point  Source
    Surface  Area Loading
Eutrophic in 1971
7.6 x 106 m2
1.7 x 106 m2
10.6 m
3.6 yr
80-123 mg/1 as CaC03
400-500 ymhos/cm @ 25°C
2.1m
< 0.005b mg P/l
0.106b mg P/l
< 0.055b mg N/l

6.0b yg/1
1370U kg P/yr
91 kg P/yr
0.86 g P/m2/yr
                Investigator-Indicated Comments
 Does not include water body surface area.
 Summer  surface  average values.
 c
 Includes urban  stormwater drainage.
JDash  (-) indicates no data available.
"Data taken  from Shapiro  (1975a)  and personal  communication
 (Table  3).
                                 406

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                      CAMELOT-SHERWOOD (WISC . )'"
           DATA SUMMARY FOR US OECD EUTROPHICATION PROJECT
                            (IMPOUNDMENT)
                    b
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus3
Mean Total Phosphorusr
Mean Inorganic Nitrogen
 (NHJ+N03+N02 as N)
Mean Chlorophyll a
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Summer Mean Epilimnetic Values :
   Total Phosphorus
   Dissolved Phosphorus
   Inorganic Nitrogen
                                   Eutrophic in 1972-1973
                                   9.1  x 107 m2
                                   2.8  x 106 m2
                                   3  m
                                   0.09 - 0.14  yr
                                   125  mg/1  as  CaC03
                                   311  ymhos/cm @ 25°C
                                   2.0m
                                   0 .008° mg P/l
                                   0.03C mg  P/l
                                   1.07
N/l
                                   6.3  yg/1  (first  two  meters  of
                                             water  column)
                                   0  kg  P/yr
                                   6600-7580  kg  P/yr
                                   2 .35-2.68  g P/m2/yr
                                   0 kg N/yr
                                   97,600  kg N/yr
                                   34.6 g  N/m2/yr

                                   0.04 mg P/l
                                   0.008 mg P/l
                                   0.59 mg N/l
                  Investigator-Indicated Comments
  Lake highly  colored because of humic content.
  Does not  include water body surface area.
  Data based on samples obtained at six-week intervals at either one
  or two meter depth intervals in the deepest part of the impoundment
  Average winter concentrations.
Dash  (-) indicates no data available.
''Data  taken from  Piwoni  and  Lee  (1975) and personal  communication
 (Table  3).
                                 407

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                          CANADARAGO  (N.Y.)
          DATA SUMMARY  FOR  US  OECD EUTROPHICATION  PROJECT
                               (LAKE)
Trophic State
rx            b
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth0
Mean Dissolved Phosphorus0
Mean Total Phosphorus0
Mean Inorganic Nitrogen0
 (NH4+N03+N02 as N)
Mean Chlorophyll a
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
                                  aEutrophic in 1968-1969
                                             2
10  m
106 m2
                                  1.8
                                  7 .6
                                  7.7m
                                  0 .6 yr
                                  248 mg/1 as
                                                   °
                                  223 ymhos/cm @ 25C
                                  1.8 m
                                  1968
                                  0 .02
                                  0.05
                                  0 .38

                                  13
                                  1971=195
       1969
       0.02 mg P/l
       0.04 mg P/l
       0.44 mg N/l

       7 yg/1
    1972=136; 1973=236
    g C/m^/yr
                                  2800 kg P/yr
                                  3200 kg P/yr
                                  0.8 g P/m2/yr
                                  7800 kg N/yr
                                  128 ,600 kg N/yr
                                  18.0 g N/m2/yr
                                                        Spring
                                                   Overturn Values
                                                    1968r96~9
                         Growing Season(May-Sept)
                         Mean Epilimnetic Values
                             1968        1969       	    	
Mean Secchi Depth (m)          -          1.7
Total Phosphorus (mg P/l)    0.06        0.04       0.02    0.03
Dissolved Phosphorus(mg P/l) 0.020       0.013      0.016   0.015
Inorganic Nitrogen (mg N/l)  0.21        0.30       0.38    0.44
Chlorophyll a (yg/1)           9           5          -       -
                Investigator-Indicated Comment^
aPrior to completion of tertiary waste treatment plant for treatment
 of major point source nutrient input in 1972.
                          (continued)
                                408

-------
       DATA SUMMARY FOR CANADARAGO (N.Y.)"- (continued)
 Does not include water body surface area.
Q
 Data based on samples obtained monthly from early May-late
 November, 1968-1969, from ten stations at the 0-4.5 m depth,
 4.5-9.0 m depth, and 9.0 bottom depth.

Dash (-) indicates no data available.
s'c
 Data taken from Hetling et al.  (1975) and persona]  communi-
 cation (Table 3).
                             409

-------
                           CAYUGA  (N.Y.)
          DATA SUMMARY FOR US OECD EUTROPHICATION PROJECT
                              (LAKE)
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus
Mean Total Phosphorus
Mean Inorganic Nitrogen
                 N)
                         .b
              as

Mean Chlorophyll a_~
Annual Primary Productivity
Phosphorus Loading
   Point Source
                  d
   Non-Point Source
   Surface Area Loading
Nitrogen Loading  :
   Point Source
   Non-Point Source
   Surface Area Loading
Secchi Depth (m)
Dissolved Phosphorus (mg P/l)
Inorganic Nitrogen (mg N/l)
Chlorophyll a (yg/1)
                                  Mesotrophic in 1972-1973
                                          q  ?
                                  2.0 x 1(T m
                                  1.7 x 108 m2
                                  54 m
                                  8 . 6 yr
                                  102 mg/1 as CaC03
                                  575 umhos/cm @ 25°C
                                  1972         1973
                                  2.3
                                  0.003
                                  0 .02
                                  0.37
2.3m
0.004 mg P/l
0.02 mg P/l
0.51 mg N/l

5 yg/1
                                  58° g C/m2/yr
                                  63,900 kg P/yr
                                  77 ,100 kg P/yr
                                  0.8 g P/m2/yr
                                  168 ,000 kg N/yr
                                  2 ,300 ,000 kg N/yr
                                  14.3  g N/m2/yr
                                     Growing Season  (May-Sept)
                                     Mean Epilimnetic Values
                                        T9T2         19T3
                                        0 .003
                                        0.35
                                        7 .4
                                                     2.4
                                                     0.001
                                                     0.36
                                                     5.6
Total Phosphorus ranges from 0.015-0.022 mg/1 throughout water
column during all seasons of the year.
                 I_nye_£tigator-I_ndicated Comments
 Data does not include water body surface area.
                        (continued)

                                 410

-------
        DATA SUMMARY FOR CAYUGA (N.Y.) - (continued)
 Data based on samples collected at three-five sampling sta-
 tions in 1972-1973, at surface, 2m, 5m and 10m, at weekly in-
 tervals during June-August, biweekly intervals during mid-
 April-May and September-October, and monthly intervals the
 rest of the year, down the long axis of the lake.

°Based on Barlow (1969) and Peterson (1971).

d!970-1971 data.
*
 Data taken from Oglesby (1975)  and personal communication
 (Table 3).

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                           CEDAR (MINN.)
          DATA SUMMARY FOR US OECD EUTROPHICATION PROJECT
                               (LAKE)
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus
Mean Total Phosphorus
Mean Inorganic Nitrogen
 (NHJ+N03 as N)
Mean Chlorophyll a
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Eutrophic in 1971
1.6 x 106 m2
6.9 x 105 m2
6.1 m
3.3 yr
71-109 mg/1 as CaCO,
400 ymhos/cm @ 25°C
1.8m
< 0.005b mg P/l
0.055b mg P/l
< 0.055b mg N/l
20^ yg/1


205° kg P/yr
36 kg P/yr
0.35 g P/m2/yr
                 Investigator-Indicated Comments

 Does not include water body surface area.
 Summer  surface average values.
 c
 Includes urban stormwater drainage.
 Dash  (-) indicates no data available.
 A
 Data taken from Shapiro (1975a) and personal communication
 (Table 3).
                                 412

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                     COX HOLLOW LAKE (WISC.) "
           DATA  SUMMARY FOR US OECD EUTROPHICATION PROJECT
                          (IMPOUNDMENT)
                          b
Trophic  State
Drainage Area
Water Body  Surface  Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth b
Mean Dissolved Phosphorus
Mean Total  Phosphorus
Mean Inorganic Nitrogen
 (NHj + NC>3+NO~ as N)
Mean Chlorophyll ab
Annual Primary Productivity
Phosphorus Loading :
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Summer Mean Epilimnetic Values:
   Total Phosphorus
   Dissolved Phosphorus
   Inorganic Nitrogen
Eutrophic in 1972-1973
1.6 x 107 m2
3.9 x 10 5 m2
3.8m
0.5 - 0.7 yr
205 mg/1 as CaCO
                3
440 ymhos/cm @ 25°C
1.5 m
0.04° mg P/l
0.10° mg P/l
0.83° mg N/l
                                   26.5 yg/1 (first two meters of
                                              water column)
                                   0 kg P/yr
                                   630-810 kg P/yr
                                   1.62-2.08 g P/m2/yr

                                   0 kg N/yr
                                   7410 kg N/yr
                                   19 .1 g N/m2/yr

                                   0.06 mg P/l
                                   0.02 mg P/l
                                   0.36 mg N/l
                Investigator-Indicated Comments
 Does not include water body surface area.
 Data based on samples obtained at six-week intervals at either
 one or two meter depth intervals in the deepest part of the im-
 poundment .
Q
 Average winter concentration.
 Dash (-) indicates no data available.
;'t
 Data taken from Piwoni and Lee (1975) and personal communication
(Table 3).
                                 413

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                           DOGFISH  (MINN.) '
          DATA  SUMMARY  FOR US  OECD EUTROPHICATION PROJECT
                               (LAKE)
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus
Mean Total Phosphorus
Mean Inorganic Nitrogen
              as N)
Oligotrophic in 1971-1972
8.8 x 10F
Mean Chlorophyll a
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
2.9 x 10
4.0 m
3.5 yr
1971
8
17 .3
2 .7

0.010
0.39

6 (4)c
          m
          m
          1972
          10 mg/1 as CaC03
          16.0 ymhos/cm @ 25°C
          2.5m
          0.010 mg P/l
              mg N/l

          4 (2)c yg/1
          0 kg P/yr
          4.9 kg P/yr
          0.02 g P/m2/yr
   Non-Point Source
   Surface Area Loading
Mean pH = 6.0
                  Investigator-Indicated Comments
Water slightly stained with humics.
Phytoplankton characterized by chrysophytes and cryptomonads except
during summer and fall, when greens  and blue greens were significant
aDoes not include water body surface area.
 May-October mean values for 1971-1972.
"Euphotic zone values.
Dash (-) indicates no data available.
 Data taken from Tarapchak et al.  (1975) and personal communication
(Table 3).

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                    DUTCH HOLLOW LAKE  (WISC.)*
          DATA SUMMARY  FOR US  OECD  EUTROPHICATION  PROJECT
                          (IMPOUNDMENT)
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth13
Mean Dissolved Phosphorus
Mean Total Phosphorus
Mean Inorganic Nitrogen
 (NHJ+N03+ N02 as N)
Mean Chlorophyll a_
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Eutrophic in 1972-1973
1.2 x 107 m2
8.5 x 105 m2
3 m
1. 8 yr
133 mg/1 as CaC03
252 ymhos/cm @ 25°C
0.8m
0.020° mg P/l
0.40C mg P/l
    °
0.61
         N/l
33.9 yg/1 (first 2 meters of water
           column)
0 kg P/yr
810-870 kg P/yr
0.95-1.01 g P/m2/yr

0 kg N/yr
8840 kg N/yr
10.4 g N/m2/yr
Summer Mean Epilimnetic Values:
   Total Phosphorus
   Dissolved Phosphorus
   Inorganic Nitrogen
0.12 mg P/l
0.01 mg P/l
0.22 mg N/l
                    Investigator-Indicated Comments
 Does not include water body surface area.
 Data based on samples obtained at six week intervals at
 either one or two meter depth intervals in the deepest
 part of the impoundment.
 Average winter concentrations.
Dash (-) No data available.
Data taken  from Piwoni and Lee  (1975) and personal communication
 (Table  3).
                                 415

-------
                            GEORGE  (N.Y.)"
          DATA  SUMMARY  FOR  US  OECD  EUTROPHICATION  PROJECT
                               ( LAKE )

Trophic State                      Oligotrophic-Mesotrophic  in  1972-73
                                           o   2
Drainage Area                      6.1  x 10   m
                                           8   2
Water Body Surface Area            1.1  x 10   m
Mean Depth                         18 m
Hydraulic Residence Time           8 yr
Mean Alkalinity                    21 mg/1 as CaC03
Mean Conductivity                  86 ymhos/cm @  25°C
Mean Secchi Depth                  8.5  m
Mean Dissolved  Phosphorus          0.002 mg P/l
Mean Total Phosphorus              0.0085 mg P/l
Mean Inorganic Nitrogen
          as N)                    0.05 mg N/l
Mean Chlorophyll a
                 —                          2
Annual Primary Productivity        7.2 g C/m /yr
Phosphorus Loading:
   Point Source                    80 kg P/yr
   Non-Point Source                7800 kg P/yr
                                             2
   Surface Area Loading            0.07 g P/m /yr
Nitrogen Loading:
   Point Source                    17,700 kg N/yr
   Non-Point Source                201,000 kg N/yr
                                            2
   Surface Area Loading            1.8 g N/m_/yr
                  Investigator-Indicated Comments
                   water body surface <
 Dash (-) indicates no data available.
Does not include water body surface area.
ft
 Data taken from Ferris and Clesceri (1975)  and personal
 communication (Table 3).
                                 416

-------
                            HARRIET (MINN.)*
          DATA  SUMMARY  FOR US  OECD EUTROPHICATION PROJECT
                               (LAKE)
Trophic State
Drainage Area
Water Body  Surface  Area
Mean Depth
Hydraulic Residence  Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus
Mean Total  Phosphorus
Mean Inorganic Nitrogen
          as N)
                                   Eutrophic in 1971
                                   4.8 x 106 m2
                                           6  2
                                   1.4x10  m
                                   8.8m
                                   2 .4 yr
                                   92  - 124 mg/1 as
                                   360-425 ymhos/cm @ 25°C
                                   2.4m
                                   <0.005b mg P/l
                                   0.062b mg P/l
                                   <0.055b mg N/l
                                      b
                                   3.
                                        yg/i
                                   890  kg P/yr
                                   126 kg P/yr
                                   0.71 g P/m2/yr
Mean Chlorophyll  a
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
                   Investigator-Indicated Comments
1Does not include water body surface area.
 Summer average surface values.
G
 Urban stormwater drainage only.
Dash (-) indicates no data available.
 Data taken from Shapiro (1975a) and personal communication
(Table 3).
b
                                417

-------
                          ISLES (MINN.)
           DATA SUMMARY FOR US OECD EUTROPHICATION PROJECT
                              (LAKE)
                                                       O,
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus
Mean Total Phosphorus
Mean Inorganic Nitrogen
 (NH4+N03 as N)
Mean Chlorophyll a_
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
                   Investigator-Indicated Comments

 Does not include water body surface area.
 Summer surface average values.
Q
 Urban storm water drainage only.
Dash (-) No data available.
*Data taken from Shapiro (1975a)  and personal  communication
 (Table 3).
Eutrophic in 1971
2.8 x 106 m2
4.2 x 105
2.7m
0.6 yr
68-131 mg/1 as CaC03
380-470 ymhos/cm @ 2!
1.0m
<0.010b mg P/l
0.110b mg P/l
<0.055b mg N/l
                                   53   yg/1
                                   828   kg  P/yr
                                   23 kg P/yr
                                   2.03  g P/m2/yr
                                 418

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                   KERR RESERVOIR (N. CAROLINA-VIR.)
           DATA SUMMARY FOR US OECD EUTROPHICATION PROJECT
                             (IMPOUNDMENT)
 Trophic State
 Drainage Areaa
 Water Body Surface Area
 Mean  Depth
 Hydraulic Residence Time
 Mean  Alkalinity0
 Mean  Conductivity °
 Mean  Secchi Depth °
 Mean  Dissolved Phosphorus0
 Mean  Total Phosphorus °
 Mean  Inorganic Nitrogen0
  (NHj+N03+NO~ as N)
 Mean  Chlorophyll ac
 Annual  Primary Productivity c
 Phosphorus  Loading:
   Point  Source
   Non-Point  Source
   Surface  Area Loading
Nitrogen  Loading:
   Point 'Source
   Non-Point  Source
   Surface  Area Loading
                  Growing Season Mean
                __ Epilimnetic Values
                06
Total Phosphorus
  (mg P/l)
Dissolved Phos-
  phorusCmg P/l)
Inorganic Nitrogen
  (mg N/l)
Chlorophyll a
  (yg/D
Primary Productiv-
  ity (g C/m2/day)
Mean Hypolimnetic D.O. Content (mg/1)
  Roanoke Arm
  Nutbush Arm
                             (continued)
                                419
Eutrophic-Mesotrophic in 1975
2.02 x 1010,m2
Roanoke Arm0   Nutbush Arm13 „
1.2 x 10»
10.3
0.2
28
100
1.4
0.01
0.03
0.28
13.2
171
630,600
13,600
5.2
18 ,500
4 ,509 ,600
36.2
5.1) x 10 ' m
8.2 m
5.1 yr
22 mg/1 as CaC03
123 ymhos/cm @ 2
1.2 m
0 .02 mg P/l
0.03 mg P/l
0.22 mg N/l
21.2 yg/1
249 g C/m2/yr
30,500 kg P/yr
5,500 kg P/yr
0 .7 g P/m2/yr
7 ,480 kg N/yr
114 ,400 kg N/yr
2 .4 g N/m2/yr
                              25°C
Roanoke Arm Nutbush Arm
s
)
gen

iv-
0.02
0 .006
0 .13
14
0.7
0
0
0
1
0
.03
.007
.10
8
.7
           Spring Overturn
       	Mean Values	
       RoanokeArmNutbush Arm
           0 .04
           0 .006
           0 .30
0 .05
0 .010
0 .20
     3/14/74   5/6/74
       9.6        6.8
      10 .8        5.1
 7/3/74
   0 . 3
   1.1

-------
                                                    ft
  DATA SUMMARY FOR KERR RESERVOIR (N. CAROLINA-VIR.) - (continued)
                 Investigator-1n d i c a t e d Commen t s

The upper ends of both arms of the reservoir are nitrogen-limited,
while the lower ends of both arms are phosphorus-limited, with
respect to algal nutrient requirements.

 Does not include water body surface area.

 The two principal arms of the impoundment have been treated
 separately.
C                                                            •
 Data based on samples obtained at approximately three-month in-
 tervals at four stations, six miles apart in the Roanoke Arm,  and
 five stations, three-five miles apart in the Nutbush Arm, during
 the period 1971-1974.  All loading estimates for April, 1974,
 March, 1975 ar.e based on monthly sampling frequency for all
 principal phosphorus inputs.

 Dash(-) indicates data not available.
ft
 Data taken from Weiss and Moore (1975) and personal communication
 (Table 3).
                                420

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                  LAMB  (MINN.)*
DATA SUMMARY FOR US OECD EUTROPHICATION PROJECT
                     (LAKE)
                        Oligotrophic in 1971-1972
                        2.0 x 10
                        4. 0 x 10!
                        4.0m
                        2. 3 yr
                        1971

                        47
                        1. 8
                        0.013
                        0. 51
m
m
      1972
      36 mg/1 as CaCO,
                        6 (5)
                             c
 Trophic State
 Drainage Area
 Water Body Surface Area
 Mean Depth
 Hydraulic Residence Time
 Mean Alkalinity
 Mean Conductivity
 Mean Secchi Depth
 Mean Dissolved Phosphorus
 Mean Total Phosphorus
 Mean Inorganic Nitrogen
  (NHiJ + NOo+NO" as N)
             /
 Mean Chlorophyll a_
 Annual Primary Productivity
 Phosphorus Loading:
    Point Source
    Non-Point Source
    Surface Area Loading
 Nitrogen Loading:
    Point Source
    Non-Point Source
    Surface Area Loading

                    Investigator-Indicated Comments
 Lake highly colored by  humic  materials.   Green  and blue-green
 algae dominates  summer  and  fall  phytoplankton community..
  Does not  include water body  surface  area.
  May-October  mean values for  1971-1972.
  Euphotic  zone.
 Dash (-) No data  available.
"Data taken from Tarapchak et_ al. (1975)  and  personal communication
 (Table 3).
                     -o.
      47 -ymhos/cm @ 25 C
      2.2m

      0.012 mg P/l
      - mg N/l

      3 (3)C ug/1
                                        0 kg P/yr
                                        12.1 kg P/yr
                                        0.03 g P/m2/yr
                      421

-------
                           MEANDER (MINN.)
           DATA SUMMARY FOR US OECD EUTROPHICATION PROJECT
                               (LAKE)
 Trophic State
 Drainage Area
 Water Body Surface Area
 Mean Depth
 Hydraulic Residence Time
 Mean Alkalinity
 Mean Conductivity
 Mean Secchi Depth
 Mean Dissolved Phosphorus
 Mean Total Phosphorus
 Mean Inorganic Nitrogen
  (NH4+N03+NOI as N)
                   1
 Mean Chlorophyll a
 Annual  Primary Productivity
 Phosphorus Loading:
    Point Source
    Non-Point Source
    Surface Area Loading
 Nitrogen Loading:
    Point Source
Oligotrophic in 1971-1972
1.7 x 106 m2
3.6 x 105 m2
5.0m
2.7 yr
1971
20.4
3.1

0.012
0 .45
1972
8 mg/1 as CaCO,
16.7 ymhos/cm {
3.0m

0.009 mg P/l
-  mg N/l

2 (l)c yg/1
           0 kg P/yr
           9.9 kg P/yr
           0.03 g P/m2/yr
25°C
    Non-Point  Source
    Surface  Area Loading
 Mean pH = 5.5
                   Investigator-Indicated Comments
 Chrysophytes  and crytomonads characterize phytoplankton,  except
 during summer and fall when green and blue-green algae-are dominant
  Does not include water body surface area.
  May-October  mean values.
  Euphotic zone.
 Dash (-) indicates no data available.
.'.
 Data taken from Tarapchak  et_ al_.  (1975)  and personal communication
(Table 3).
                                422

-------
                          MENDOTA (WISC.)
          DATA SUMMARY FOR US  OECD  EUTROPHICATION PROJECT
                               (LAKE)
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus
                     .b
Mean Total Phosphorus
Mean Inorganic Nitrogen
              as N)
                       b
                  c
Mean Chlorophyll a
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Euphotic zone = to 3 m depth
Euphotic zone volume = 9 x 10
Summer eiplimnion mean depth =
Summer epilimnion mean volume
                                  Eutrophic in 1965-1966
                                  6.9 x 108 m2
                                  3.9 x 107 m2
12 m
4 . 5 yr
160 mg/1 as CaCO
300 ymhos/cm
3.0m
0.12 mg P/l
0.15 mg P/l
0.64 mg N/l
                                                 25°C
                                  10 (20)  yg/1
                                  1100e g C/m2/yr

                                  908f kg P/yr
                                  45,600 kg P/yr
                                  1.2 g P/m2/yr

                                  3130f kg N/yr
                                  540 ,700 kg N/yr
                                  13 g N/m2/yr
                             7
                               m
                               to 10 m
                              -  3 x 10?
                                        x m
                   Investigator-Indicated Comments^
 Does not include water body surface area.
 Based on 1965-1966 study by students and staff of Water Chemistry
 Program, Univ.  of Wisconsin,  Madison, and  compiled by Lee (1966).
-i
'Mean epilimnetic concentration.

                            (continued)
                                423

-------
          DATA SUMMARY FOR MENDOTA (WISC.)"- (continued)
 Growing  season concentration.
 Estimated from chlorophyll  and  light  intensity data.
 Point  source loadings  are mainly storm water drainage inputs.
&
 Data taken from Lopez  and Lee (1975)  and personal communication
 (Table 3).

-------
                         MICHIGAN  (MICH.)
          DATA SUMMARY  FOR US  OECD EUTROPHICATION PROJECT
                               (LAKE)
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus
Mean Total Phosphorus
Mean Inorganic Nitrogen
 (NHj + NC>3+N0~ as N)
Mean Chlorophyll a
Annual Primary Productivity
Phosphorus Loading6:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading6:
   Point Source
   Non-Point Source
   Surface Area Loading
Euphotic  zone =  8 m
                                   Nearshore-Mesotrophic  in  1972
                                   Open  waters-Oligotrophic  in  1974
                                   1  Q v 1 nil  m2
                                   1.8  x 10
                                   5.8  x 10
                                   84 m
   nr
10 m2
                                   30-100  yrh
                                   Nearshore^
                                   107
                                   265
                                   2.3
                                   <0. 002
                                   0.015
                                   0.20
                                   187-247
     Open Waters
     113 mg/1 as CaC03
     255 ymhos/cm @ 25°C
        m
                                       1971
                                       1974
     0.001  mg P/l
     0.013° mg P/l
     0.17° mg N/l
     2  yg/i
     150d g C/m2/yr
     3.4 x 10  kg P/yr
     2.2 x 106 kg P/yr
     0.14 g P/m2/yr
     0.10 g P/m2/yr
                                       1971 =    1.3 g N/m /yr
b
                   Investigator-Indicated Comments
  Does  not include  water body surface  area.
  Data  based on  samples  obtained  at  the  four meter  depth from one
  station  over an  18 month  period in 1970-1971.
 CAfter Schelske and Callender (1970).
 dAfter Vollenweider (1975a).
 SAfter Lee (1974a).
  Dash(-)  indicates no data available.
&
 Data taken from Piwoni et  al. (1976)  and personal  communication
(Table  3).
                                 425

-------
                     LOWER LAKE  MINNETONKA (MINN.)
          DATA SUMMARY FOR US OECD  EUTROPHICATION  PROJECT
                               (LAKE)
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus
Mean Total Phosphorus
Mean Inorganic Nitrogen
 (NHJ+N03 +N0~ as N)
Mean Chlorophyll a
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Eutrophica in 1973
3.7 x 108 m2
2.62 x 107 m2
 .3m
 .3°
1969
6.3° yr
125
1.5

0.06
21
440

8900
4000
0.5
                            Growing Season Mean
                            Epilimnetic Values
1973
-  mg/1 as CaC03
125 ymhos cm @ 25°C
1.8 m
0.003 mg P/l
0.05 mg P/l
             12 yg/1
             320 g C/m2/yr

             0 kg P/yr
             2800 kg P/yr
             0.1 g P/m2/yr
              (0.2)f
               Spring Overturn
                 Mean Values
 Secchi Depth (m)
 Total Phosphorus
   (mg P/l)
 Chlorophyll a (yg/1)
 PhotosynthetTc Rate
   (g C/m2/day)
1969
1.4
0.05
23
2 .5
1973 1972
1.7
0 .04 . 0.08
15
1.9
1973
0 .04
-
                              (continued)
                                426

-------
 DATA SUMMARY FOR LOWER LAKE MINNETONKA  (MINN.)"-  (continued)


                 Investigator-Indicated  Comments

aTrophic status as of 1973.  Sewage diversion was  begun during win-
 ter of 1971-1972, eliminating the point source phosphorus input.
 Prior to sewage diversion, lake was considered eutrophic.  Lower
 Lake Minnetonka is still considered eutrophic in  1973.  However,
 the decreasing nutrient and chlorophyll concentrations and
 primary productivity and increasing Secchi depth  observed in
 1973-1974, relative to the 1969 values, indicate  the lake to
 be changing to a less fertile trophic condition.

 Does not include water body surface area.
c                           •                       •
 Watershed area and hydraulic residence  time data  is for entire
 lake.  All other data is only for Lower Lake Minnetonka.  It was
 not possible to calculate hydraulic retention times for individual
 basins.  Thus, the hydraulic residence  time for the whole lake was
 used in all calculations.

 Data obtained from samples obtained during the 210-day ice-free
 period, on ten dates in 1969 and seven  dates in 1973, at five
 meter depth intervals from the surface  to the bottom of the lake.
Q
 Data obtained from samples incubated at 0, 0.5, 1.0, 2.0, 3.0 and
 5.0 meter depths on eight dates between April 25-November 11,
 1969 and 1973.

 Data in parentheses represents data received by these reviewers
from the principal investigator subsequent to completion of this
report.   Examination of the revised data indicated no significant
changes  in the overall conclusions concerning Lake Minnetonka.

Dash (-) indicates no data available.

*
 Data taken from Megard (1975)  and personal communication
                                427

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               POTOMAC ESTUARY (MARYLAND, VIRGINIA)
          DATA SUMMARY FOR US OECD EUTROPHICATION  PROJECT
                              (ESTUARY)
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth0
Mean Dissolved Phosphorus0
Mean Total Phosphorus0
Mean Inorganic Nitrogen0
              as N)
Mean Chlorophyll a_
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
                                      Ultra-eutrophic  in  1966-1970
                                            3.8  x 1010 m2
                                Upper Reach  Middle  Reach
                Lower Reach
                                 5.7  x 10'
                                 4.8
                                 0.04
                                 70-110
                                 200-500
                                 0.4-0.8
                                 0.2-0.8
                                 0.3-1.2
                                 1.8-3.2

                                 30-150
2.1 x
5.1
  7.0 x 108 m2
  7.2m
0.18            0.85 yr
60-85     65-85 mg/1 as CaCO.
600-17,000
0.5-1.3
0.08-0.15    0.01-0.04 mg P/l
0.01-0.75    0.03-0.06 mg P/l
0.15-0.33    0.05-0.15 mg N/l
17,000-26,000
yrrmo s / cm@ 2 5° C
1.0-2.3 m
30-100
10-20 yg/1
                                 4.0 x 10  kg P/yr
                                 8.8 x 105 kg P/yr
                                 85          8            _._   _..
                                 (For total estuary = 5 g P/m^/yr)
                                 9.9 x 106 kg N/yr
                                 6.6 x 106 kg N/yr
             1.2 g P/m /yr
                                 288         32           25
                                 (For total estuary - 17.2 g
                   Investigator-Indicated Comments
Lower estuary is saline.
Dominant algae is Anacystis.
The dissolved oxygen content  is low in the upper and lower reach.
                                                                  2
                                                                   The
upper and middle reaches become nitrogen-limited with respect to aquatic
plant nutrient requirements during the summer months .
 Does not include water body surface area.
 The estuary has been divided into three separate regions (reaches).
 Each reach is treated separately.
                            (continued)
                                428

-------
DATA SUMMARY FOR POTOMAC ESTUARY  (MARYLAND,  VIRGINIA)*-  (continued)
Q
 June through  September values;  data based  on  samples  obtained  at
 monthly intervals between  1966-1969, and weekly  intervals  between
 1969-1970, at the top and  bottom  sampling  depths,  from  sampling
 stations at five mile intervals in the  upper  estuary  and larger
 intervals  in  the lower estuary.
Dash  (-) No data available.
*
 Data taken from Jaworski  (1975)  and personal communication
 (Table  3).
                               429

-------
                      LAKE REDSTONE  (WISC.)"
          DATA SUMMARY FOR US OECD EUTROPHICATION  PROJECT
                          (IMPOUNDMENT)
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depthb
Mean Dissolved Phosphorus
                     V
Mean Total Phosphorus
                         b
Mean Inorganic Nitrogen
 (NH4+N03+N02 as N)
Mean Chlorophyll a_
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Summer Mean Epilimnetic Volumes
   Total Phosphorus
   Dissolved Phosphorus
   Inorganic Nitrogen
Eutrophic in 1972-1973
7.7 x 107 m2
2.5 x 106 m2
4.3m
0.7-1.0 yr
125 mg/1 as CaCO
260 ymhos/cm @ 25°C
1.6 m
0.008C mg P/l
0.03° mg P/l
0.80° mg N/l
                                 12.8  yg/1 (first two meters
                                 of water column)
                                 0 kg P/yr
                                 3630-4230 kg P/yr
                                 1.44-1.68 g P/m2/yr

                                 0 kg N/yr
                                 45,400 kg N/yr
                                 18.1 g N/m2/yr
                                 0.11 mg P/l
                                 0.008 mg P/l
                                 0.30 mg N/l
b
                   Investigator-Indicated Comments
 ""Does not include water body surface area.
 Data based on samples obtained at six week intervals at either
 one or two meter depth intervals in the deepest part of the
 impoundment.
c
 Average winter concentration.
Dash (-) No data available.
 Data taken from Piwoni and  Lee (1975)  and  personal  communication
 (Table  3).

-------
                        LAKE  SALLIE  (MINN.)
          DATA SUMMARY  FOR US  OECD  EUTROPHICATION PROJECT
                               (LAKE)
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus
Mean Total Phosphorus
Mean Inorgan_ic Nitrogen
            ' as N)
Mean Chlorophyll a_
Annual Primary Productivity
                  c
Phosphorus Loading  :
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading0 :
   Point Source
   Non-Point Source
   Surface Area Loading
Eutrophic in 1968-1972
        9  2
1.5 x 10  m
5.3 x 106 m2
6.4m
1.1-1.8 yr
162 mg/1 as CaCO
                O
280-360 ymhos/cm @ 25 C

0.13 mg P/l
0.35 mg P/l
0.44 mg N/l
7060-20,080 kg P/yr
1030-1970 kg P/yr
1.5-4.2 g P/m2/yr

5590-11,360 kg N/yr
4195-9086 kg N/yr
2.8-3.0 g N/m2/yr
                               Growing Season
                               (May-September)
                          Mean Epilimnetic Values
Total Phosphorus (mg P/l)
Dissolved Phosphorus (mg P/l)
Inorganic Nitrogen (mg N/l)
Primary Productivity
 (mg C/m^/Langley/hr)


/I)
)
1972
0.4
0. 04
0.15
1973
0.65
0.20
0.18
                   Spring Overturn
                     Mean Values
                        1.12
                        0. 26
                        0.70
 9.6
9.6
                               (continued)
                                  431

-------
       DATA SUMMARY FOR LAKE SALLIE (MINN.)''- (continued)
                 Investigator-Indicated Comments

Hypolimnion does not persist over a growing season

 Does not include water body surface area.

 Data based on samples obtained at weekly intervals during 1972-
 1973 at 22 stations located at the lake inlet and outlet, on
 a transect down the middle of the lake, and around the shore line

C1968-1972 data.
Dash (-) No data available.
it
 Data taken from Neel  (1975) and  personal communication
 (Table  3).
                                M-32

-------
                         SAMMAMISH (WASH.)
          DATA SUMMARY  FOR US  OECD EUTROPHICATION PROJECT
                               (LAKE)
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus0
Mean Total Phosphorus0
Mean_Inorganic Nitrogen0
 (N03+N02 as N)
Mean Chlorophyll a_
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
Mesotrophic  in 1970-1975
2.7 x 108 m2
        7  2
2.0 x 10  m
18 m
1. 8 yr
33 mg/1 as CaCOQ
               O
94 umhos/cm @ 25 C
3.3m
0.006 mg P/l
0.03 mg P/l
0.18 mg N/l

5 yg/1
238 g C/m2/yr

500 kg P/yr
12,500 kg P/yr
         2
0.7 g P/m /yr

0 kg N/yr
258,000 kg N/yr
                                  13.0 g N/m /yr
   Non-Point  Source
   Surface  Area  Loading
                           Growing Season
                          (March - August)
                      Mean Epilimnetic Values
Secchi Depth (m)                  3.3
Total Phosphorus (mg P/l)         0.03
Dissolved Phosphorus (mg P/l)      0.004
Inorganic Nitrogen (mg N/l)       0.24
Chlorophyll a (yg/1)              6
                           2
Primary Productivity (g C/m /day) Q.7
                                                              2
Growing Season Hypolimnetic Oxygen Depletion Rate = 0.05 mg/cm /day
(constant from year to year)
                             (continued)
                                 433
                                                 Winter  (Dec.-Feb.)
                                                   Mean  Values
                                                   (photic zone)
                                                         3.0
                                                         0.03
                                                         0.013

-------
                                           s't

        DATA SUMMARY FOR SAMMAMISH  (WASH.)  -  (continued)
                  Investigator-Indicated Comments

 Partial wastewater input diversion  («30% of total phosphorus
 input) begun in 1968.

 Does not include water body surface area.
/-»
 Data based on photic zone  (7.3 m) measurements.

 Post-sewage diversion nutrient loadings.  Pre-sewage  diversions
 are as follows:  total phosphorus = 20,000 g/yr = 1 g/m2/yr
                  total nitrogen = 243,000 kg/yr = 12.3 g/m2/yr

Dash (-) No data available.
*
 Data taken from Welch et al. (1975) and personal communication
 (Table 3).
                                 434

-------
                           SHAGAWA  (MINN.)
          DATA SUMMARY  FOR US  OECD EUTROPHICATION PROJECT
                               (LAKE)
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth0
Mean Dissolved Phosphorus1"
Mean Total Phosphorus0
Mean Inorganic Nitrogen
 (NH^+NO^+NO" as N)
Mean Chlorophyll a°
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
       Eutrophic
       2.7 x 108 m
           in 1972
           2
d
9.2 x 106 m2
5.7m
0. 8 yr
22  (fall circulation) mg/1 as CaC03
60  (fall circulation) ymhos/cm @ 25 C
2.3 (ice-free period) m
0.021 mg P/l
0.06 mg P/l
0.160 mg N/l

15  (24)d yg/1
220 g C/m2/yr

5100 kg P/yr
1150 kg P/yr
0.7 g P/m2/yr

20,000 kg N/yr
52 ,000 kg N/yr
7.8 g N/m2/yr
                             Growing  Season
                             (May-September)
                        Mean Epilimnetic Values
                         Spring Overturn
                           Mean Values
  Secchi Depth  (m)                  1.7                   2.1
  Total Phosphorus  (mg  P/l)         0.05                  0.05
  Dissolved Phosphorus  (mg P/l)     0.005                 0.024
  Inorganic Nitrogen  (mg N/l)       0.04                  0.20
  Chlorophyll a  (yg/1)              31                   13.0
  1972 Growing  Season Hypolimnetic  Oxygen Depletion Rate =1.0 mg/1/week
  (assumed constant growing season  hypolimnion volume)
                             (continued)
                                 435

-------
          DATA SUMMARY FOR SHAGAWA (MINN.)*- (continued)
                   Investigator-Indicated Comments

 Prior to completion of tertiary waste treatment plant for input
 wastewater discharges in 1972-1973.

 Does not include water body surface area.
Q
 Data based on samples obtained from three stations at 1.5 m
 depth intervals from surface to bottom

 Ice-free period averages.

Dash (-) No data available.
A
 Data taken from Malueg et al.  (1975)  and personal communication
(Table 3).

-------
                         LAKE STEWART (WISC.)
           DATA SUMMARY FOR US OECD EUTROPHICATION PROJECT
                           (IMPOUNDMENT)
Trophic State
Drainage Area
Water Body  Surface  Area
Mean Depth
Hydraulic Residence  Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus
Mean Total  Phosphorus
Mean Inorganic Nitrogen
  (NHL| +N0g +N0o as N)
Mean Chlorophyll ab
Annual Primary Productivity
Phosphorus  Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Summer  Mean  Epilimnetic Values
   Total  Phosphorus
   Dissolved Phosphorus
   Inorganic Nitrogen
                                    Eutrophic in 1972-1973
                                    2.1 x 106 m2
                                    2.5 x 104 m2
                                    1.9m
                                    0.08 yr
                                    213 mg/1 as CaCO.
                                                    O
                                    540 ymhos/cm @ 25°C
                                    1.4 m
                                    0.001° mg P/l
                                    0.04° mg P/l
                                    2.26° mg N/l
                                    12.3 yg/1 (first two meters of
                                    water column)
                                    0 kg P/yr
                                    121-202 kg P/yr
                                    4. 82-8.05 g P/m2/yr

                                    0 kg N/yr
                                    1850 kg N/yr
                                    73.6 g N/m2/yr
                                    0.08 mg P/l
                                    0.008 mg P/l
                                    0.86 mg N/l
                    Investigator-Indicated Comments
 Does not include water body surface area.
 Data based on samples obtained at six week intervals at either
 one or two meter depth intervals in the deepest part of the im-
 poundment .
c
 Average winter concentration.
tDash  (-) No data available.
Data  taken from Piwoni and  Lee  (1975) and  personal  communication
 (Table  3).
                                  437

-------
                       TAHOE (CALIF., NEVADA)
          DATA SUMMARY FOR US OECD EUTROPHICATION  PROJECT
                              (LAKE)
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus
Mean Total Phosphorus
Mean Inorganic Nitrogen
                 N)
                  b
 (NH4+N03+N02 as
Mean Chlorophyll a
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Growing Season (May-September)
Mean Epilimnetic Values:
   Secchi Depth (m)
   Total Phosphorus (mg P/l)
   Dissolved Phosphorus (mg P/l)
   Inorganic Nitrogen (mg N/l)
   Chlorophyll a (yg/1)
                              2
   Primary Productivity (g C/m /day)
                                      Ultra-oligotrophic in 1973-1974
                                              9  ?
                                      1.3 x 10  m
                                      5.0 x 108 m2
                                      313 m
                                      700 yr
                                      43 mg/1 as CaCO
                                                     O
                                      92 umhos/cm @ 25°C
                                      28.3m
                                      <0.005 mg P/l (non-detectable)
                                      0.003 mg P/l
                                      0.02 mg N/l
                                      0.3 yg/1 (euphotic zone)
                                               2
                                      5.6 g C/m /yr
                                      0 kg P/yr
                                      23,400 kg P/yr
                                      0.05 g P/m2/yr
                                      0 kg N/yr
                                      257,300 kg N/yr
                                      0. 52 g N/m2/yr
                                      1973
1974
22.5
0.003
<0.003
0.006
-
0. 05
24.3
0.003
<0.003
0.003
0.2
0. 03
                                      (6 year euphotic zone average =
                                       0.15)
                             (continued)
                                 438

-------
                                               *
       DATA SUMMARY FOR TAHOE  (CALIF., NEVADA) -  (continued)
                  Investigator-Indicated Comments

 Does not include water body  surface area.

 Data based on samples obtained at monthly intervals during
 1973-1974, at the deep midlake stations from twelve depths
 between 0 and 400 meters.  The chlorophyll value is only for
 1974.
Q
 Six-year average value

 Data based on samples obtained weekly to tri-monthly between
 August, 1967 and December, 1971 at 13 depths between 0 and 105 m
 (euphotic zone).

Dash  (-) No data available.
ft
 Data taken  from Goldman  (1975)  and personal  communication
 (Table  3).
                                 439

-------
                       EAST TWIN LAKE (OHIO)
          DATA SUMMARY  FOR  US  OECD EUTROPHICATION PROJECT
                               (LAKE)
Trophic State
Drainage Area"
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus
Mean Total Phosphorus
Mean Inorganic Nitrogen
 (NH4+N03+N07 as N)
                  r\
Mean Chlorophyll a_
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
 Eutrophic in 1972-1974
3.3 x 106  m2
2.7 x 105  m2
5.0m
1971
-
~
-

2.1
0. 05
0. 09
1. 34

1972
0 .8
~
374

1.6
0.03
0. 08
0. 58

1973
0.9
105
380

2. 3
0. 04
0. 08
0. 84

1974°
0 .5 yr
105 mg/1 as CaC03
366 ymhos/cm
@ 25oC
1.9m
0.02 mg P/l
0.08 mg P/l
mg N/l
 21
26
         22
Ug/1
                  4748 g C/m2/yr
                    0  kg P/yr
 -  192 (18lfl39(127)185(220)kg P/yr
 -  0.7 (0.7^0. 5(0. 5)0. 7(0. 8) g P/m2/vr
   0
8340
31.4
      0
   5190
   19.3
                    -  kg N/yr
                    -  kg N/yr
                    -  g N/m2/yr
                   Investigator Indicated  Comments
 1Sewage diversion begun in late 1971-1972.   Lake was  considered
 early eutrophic prior to sewage diversion.   Lake is  still  con-
 sidered eutrophic at present time.  However,  the changing  char-
 acter of the plankton populations indicate  the lake  to  be  changing
 toward a mesotrophic state.
 East Twin Lake and West Twin Lake are  connected by a tributary  and
 share the same watershed drainage area.   Drainage area  does  not
 include water body surface area.
 •»
 'Experienced sewage leak from West Twin Lake- into East Twin Lake
 in 1974.
                            (continued)
                                440

-------
       DATA SUMMARY FOR EAST TWIN LAKE (OHIO)" - (continued)
  Data based on samples obtained from the deepest point in each
  lake, generally weekly from late spring - early fall, and less
  frequently the rest of the year, at 0.1, 2, 4, 7, and 10 meters
  from 1971-1974.

  Average of 6 measurements made between June 27, 1974 - August 9,
  1974.  An in situ measurement technique used because of diffi-
  culty of estimating primary productivity of extensive macrophyte
  production.

 Summer season mean epilimnetic nutrient concentrations given in
 Cooke et aJL. (1975)
 Dash (-) No data available.

 All data in parentheses  represents data received by these reviewers
  from the  principal investigators subsequent to completion of
  this report.   The  original data supplied by the investigator
  was used  in all figures  in this report.   Examination of the
  revised data indicated no significant  changes in the overall
  conclusions concerning East Twin Lake.
it
Data taken from Cooke et  a1. (1975)  and personal communication
 (Table 3).
                                441

-------
                      WEST TWIN LAKE (OHIO)
          DATA SUMMARY FOR US  OECD  EUTROPHICATION PROJECT
                              (LAKE)
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depthc
                          c
Mean Dissolved Phosphorus
Mean Total Phosphorus
                        c
Mean Inorganic Nitrogen
  (NH4+N03+NO~ as N)
            ^      Q
Mean Chlorophyll a
Annual  Primary Productivity
Phosphorus Loading:
    Point Source
    Non-Point  Source
    Surface Area  Loading
Nitrogen Loading:
    Point Source
    Non-Point  Source
    Surface Area  Loading
                                 Eutrophic  in 1974
                                 3.3 x  106 m2
                                 3.4 x  105 m2
                                 4. 34 m
                                 1971   1972  1973    1974

-


1
.6

1
.8
110

0
0
1
1.
.0
7
7
.15
.9
3
411
2.2
0
0
0
•
•
.
06
12
79
0
0
0
4
2
.
•
•
09
.8
06
11
83
1.0
106
380
2.3
0.04
0.10
-
yr
mg/1 as
ymhos/cm
in 25°C
mg P/l
mg P/l
mg N/l

CaCO




                                    27     40     23      28  ug/1
                                                      576d g C/m2/yr
                                          0      0       0   kg P/yr
                                    -  118(143^103(61)91(107) kg P/yr
                                    -  0.4(0.4)0.3 (0.2)0.3(0.3')gP/m2/yr
                                          0
                                        5457
                                          16
  0
5094
  15
kg N/yr
g N/m /yr
                  Investigator-Indicated  Comments
b
 Sewage diversion begun  in  late  1971-1972.   Lake  was  considered
 eutrophic prior to  sewage  diversion.   However,  lake  is considered
 mesotrophic at the  present time because  of  its  changing plankton
 characteristics.
 East Twin and West  Twin Lake  are connected  by a  tributary and
 share the same watershed drainage area.   Drainage area does not
 include water body  surface area.
-i
"Data based on samples obtained  from the  deepest  point in each lake,
 generally weekly from late spring-early  fall, and less frequently
 the rest of the year at 0.1,  2, 4,  7  and 10 meters from 1971-1974.
                            (continued)
                                442

-------
           DATA SUMMARY FOR WEST TWIN LAKE (OHIO)"- (continued)


 All data in parentheses represents data received by these re-
 viewers from the principal investigator subsequent to completion
 of this report.   The original data supplied by the investigator
 was used in all figures in this report.  Examination of the re-
 vised data indicated no significant changes in the overall
 conclusions concerning West Twin Lake.

Summer season mean epilimnetic nutrient  concentrations given in
Cooke et al. (1975).

Dash (-) No data available.

*
 Data taken from Cooke et al.  (1975) and personal communication
(Table 3).

-------
                      TWIN VALLEY LAKE (WISC.)
          DATA SUMMARY FOR US  OECD  EUTROPHICATION PROJECT
                           (IMPOUNDMENT)
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus
Mean Total Phosphorus
Mean Inorganic Nitrogen
 (NHiJ + NOs+NO' as N)
Mean Chlorophyll a_
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Summer Mean Epilimnetic Values
   Total Phosphorus
   Dissolved Phosphorus
   Inorganic Nitrogen
Eutrophic in 1972-1973
3.1 x 107 m2
6.1 x ID5 m2
3.8m
0.4-0.5 yr
175 mg/1 as CaC03
370 ymhos/cm @ 25°C
1.5 m
0.019° mg P/l
0.07° mg P/l
0.51° mg P/l

19 ug/1 (first two meters of
   water column)
0 kg P/yr
1090-1250 kg P/yr
1.74-2.05 g P/m2/yr
0 kg N/yr
10,500 kg N/yr
17.4 g N/m2/yr
0.06 mg P/l
0.01 mg P/l
0.23 mg N/l
                   Investigator-Indicated Comments
 Does not include water body surface area".
 Data based on samples obtained at six week intervals, at
 either one or two meter depth intervals,- in the deepest
 part of the impoundment.
Q
 Average winter concentrations.
Dash (-) No data available.
&
 Data taken from Piwoni and Lee (1975) and personal communication
(Table  3).
                                 444

-------
                                            ft
                       LAKE VIRGINIA  (WISC.)
           DATA SUMMARY FOR US OECD EUTROPHICATION PROJECT
                       (SEEPAGE IMPOUNDMENT)
                          b
Trophic State
Drainage Area
Water Body Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus
Mean Total Phosphorus
Mean Inorganic Nitrogen
 CNH4+N03+N02  as N)
Mean Chlorophyll a_
Annual Primary Productivity
Phosphorus Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrogen Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Summer Mean Epilimnetic Values:
   Total Phosphorus
   Dissolved Phosphorus
   Inorganic Nitrogen
Eutrophic in 1972-1973
        c  o
6.5x10  m
1.8 x 105 m2
1.7m
0.9-2.8 yr
64 mg/1 as CaCO
230 ymhos/cm @ 25°C
1.2m
0.004° mg P/l
0.02C mg P/l
0.22° mg P/l
                                   29.0 yg/1  (first two meters
                                       of water column)
                                   0 kg  P/yr
                                   210-270 kg P/yr
                                   1.15-1.48 g  P/m2/yr
                                   0 kg N/yr
                                   3300 kg  N/yr
                                   18.3 g N/m2/yr
                                   0.15 mg  P/l
                                   0.025 mg P/l
                                   0.18 mg  N/l
                   Investigator-Indicated  Comments
  Does  not  include  water body  surface  area.
  Data  based on  samples obtained at  six week  intervals at  either
  one or  two meter  depth intervals in  the  deepest part of  the
  impoundment.
 Q
  Average winter concentration.
 Dash  (-) No data available.
ft
 Data taken from Piwoni and Lee (1975) and personal communication
  (Table 3).

-------
                           WALDO (ORE.)'
          DATA SUMMARY FOR US OECD EUTROPHICATION PROJECT
                              (LAKE)
                         b
Trophic  State
Drainage Area
Water Body  Surface  Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus'
Mean Total  Phosphorus
Mean Inorganic Nitrogen
  (NH4+N03+NO~ as N)
Mean Chlorophyll a_
Annual  Primary Productivity6
                   e
Phosphorus  Loading   :
    Point Source
    Non-Point  Source
    Surface  Area  Loading
Nitrogen Loading  :
    Point Source
    Non-Point  Source
    Surface  Area  Loading
Ultra-Oligotrophic in 1974
7.9 x 10? m2
2.7 x 107 m2
36 m
21 yr
1.8 mg/1 as CaC03
3.4 ymhos/cm @ 25°C
28 m
<0.005 mg P/l
<0.005 mg P/l
<0.010 mg N/l
                                 0.32
                                 0.001-0.003 g C/m2/dayd
                                 0 kg P/yr
                                 458 kg P/yr
                                 0.017 g P/m2/yr

                                 0 kg N/yr
                                 9020 kg N/yr
                                 0.33 g N/m2/yr
b
c
                   Investigator-Indicated Comments
  Does  not  include water body surface area.
  Data  based on samples  obtained from nine stations each August
  from  1970 to 1974,  at  20  meter depth intervals.   Significant
  differences between epilimnetic and hypolimnetic values do not
  appear to exist.
  Average of summer measurements for 1969, 1970,  and 1974.
  Summer 1970 value.
 Q
  Based on  average of four  indirect calculation methods.
  (see  Powers et al., 1975)
  Based on  average of two indirect calculation methods.
  (see  Powers ejt al. , 1975)
*Data taken from Powers  et_ al. (1975) and personal communication
(Table 3).

-------
                        WASHINGTON (WASH.)
          DATA  SUMMARY FOR US OECD EUTROPHICATION PROJECT
                              (LAKE)
Trophic  State
Drainage Area*5
Water  Body  Surface  Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi Depth
Mean Dissolved Phosphorus
Mean Total  Phosphorus
Mean Inorganic Nitrogen
 (NHj+N03+NO~ as N)
Mean Chlorophyll a_
Annual Primary Productivity
Phosphorus  Loading:
   Point Source
   Non-Point Source
   Surface Area Loading
Nitrop.en Loading:
   Point Source
   Non-Point Source          l,
   Surface Area Loading
Growing Season (May-Sept.)
Mean Epilimnetic  Values:
   Total Phosphorus  (mg P/l)
   Dissolved Phosphorus (mg P/l
   Inorganic Nitrogen (mg  N/l)
   Chlorophyll a  (jig/l)
  Mesotrophica in 1974
          Q  9
  1.6 x 10  m
  8.8 x 107 m2
  33 m
  2 .4 yr
  45 mg/1 as CaCO_
                 J,
  81 ymhos/cm @ 25
1S33
-
0.003
0.016
0.007
_
—
1957°
1957
2.2
0.002
0.024
0.12
12
—
1964°
1963-4
1.2
0.030
0.066
0.24
20
766
1971d
1971
3.5
0.006
0.018
0.18
6
354
1974d
1974
3.8m
(3.5 m)e
- mg P/l
- mg N/l
(l4)e
- g C/m2/
yr
 57,100 103,900   0
 60,400  98,500 37,600
   1.2     2.3    0.43

201,700 271,000    0
487,200 418,200 401,600
  19.2     7.8    4.6

         1933    1957
        0.013   0.022
)        0.001   0.002
        0.037   0.042
                15
   0    kg P/yr
 41,300 kg P/yr
  0.47  g P/m2/yr
   0    kg N/yr
386,900 kg N/yr
  4.4   g N/m2/yr
 1963   1971
0.060  0.014
0.010  0.005
0.106  0.067
 29      9
                           (continued)
                                447

-------
         DATA SUMMARY FOR WASHINGTON (WASH.) - (continued)
                  Investigator-Indicated Comments

 Sewage diversion project begun in 1963 and completed in 1968.
 Lake Washington was considered eutrophic prior to 1963.  However,
 the nutrient and chlorophyll concentrations and primary pro-
 ductivity have decreased dramatically since 1963, indicating
 a much lower fertility as a result of the sewage diversion
 project.   Lake Washington is considered mesotrophic at the
 present time.

 Does not include water body surface area.
c           ....
 Maximum estimated input, including septic tank drainage.  However,
 part of this would already have been measured in the stream in-
 puts, and therefore this estimate may be slightly higher than the
 actual input phosphorus loading.
 Post-sewage diversion loading of the two major outlets; does
 not include storm water drainage overflow, which is not considered
 a major nutrient input source.

 Data in parentheses represents data received by these reviewers
 from the principal investigators subsequent to completion of this
 report.  Examination of the data indicates no significant changes
 in the overall conclusions concerning the water body.

Dash (-) No data available.

"Data taken from Edmondson (1975a) and personal communication
 (Table 3).

-------
                            WEIR  (FLA.)
           DATA SUMMARY FOR US OECD EUTROPHICATION  PROJECT
                               (LAKE)
Trophic  State
Drainage  Area
V'ater  Body  Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi  Depth
Mean Dissolved Phosphorus
Mean Total  Phosphorus
                        b
Mean Inorganic Nitrogen
  (NH^ + NO^+NO" as N)
Mean Chlorophyll  a_
Annual Primary Productivity
Phosphorus  Loading:
   Point  Source
   ICon-Point  Source
   Surface Area  Loading
Nitrogen  Loading:
   Point  Source
   Non-Point  Source
   Surface Area  Loading
May-September Mean Epilimnetic
   Values:
   Secchi Depth
   Total Phosphorus
   Dissolved Phosphorus
   Inorganic Nitrogen
   Chlorophyll a
   Primary Productivity
Mesotrophic in 1974-75
        7  9
4.6 x 10  m
2.4 x 107 m2
6.3m
4 . 2 yr
11.5 mg/1 as CaCO
133 ymhos/cm @ 25 C
1.9 m
0.025 (0.006)  mg P/l
0.08 (0.02)C mg P/l
0.07 (0.20)° mg N/l
8 (6)^ yg/1
        2
36 g C/m /yr

0 kg P/yr
3290 kg P/yr
0.14 g P/m2/yr

0 kg N/yr
61,920 kg N/yr
2.6 g N/m2/yr
1.9m
0.08 mg P/l
0.022 mg P/l
0.04 mg N/l
4 yg/1
0.4 g C/m2/day
                            (continued)
                                 449

-------
             DATA SUMMAFY FOR WEIF (FLA.) - (continued)
                  Investigator-Indicated Comments


 Does not include water body surface area.

 Data based on samples obtained at biweekly intervals at three
 stations at the surface, 1m, 3m, 5m, and at station 1, 7m depths,
 from 6/20/74 to 1/19/75.
Q
 1969-70 average values.

*Data taken from Brezonik and Messer (1975)  and personal communication
 (Table 3).
                                 450

-------
                          WINGRA (WISC.)
           DATA SUMMARY FOR US OECD EUTROPHICATION PROJECT
                              (LAKE)
Trophic  State
Drainage Area
Water  Body  Surface Area
Mean Depth
Hydraulic Residence Time
Mean Alkalinity
Mean Conductivity
Mean Secchi  Depth
Mean Dissolved  Phosphorus
Mean Total  Phosphorus
Mean Inorganic  Nitrogen
           as N)
Mean  Chlorophyll  a_
Annual  Primary  Productivity
Phosphorus  Loading:
    Point  Source
    Non-Point  Source
    Surface  Area Loading
Nitrogen  Loading:
    Point  Source
    Non-Point  Source
    Surface  Area Loading
Growing Season (May-September)
Mean Epilimnetic Values:
   Total Phosphorus
   Dissolved Phosphorus
   Inorganic Nitrogen
   Primary Productivity
                                   Eutrophic  in  1970-1971
                                           7   2
                                   1.4 x 10  m
                                   1.4 x 106  m2
                                   2.4m
                                   0. 4 yr
                                   153 mg/1 as CaC00
                                   1.3m
                                   0.02  mg P/l
                                   0.07  mg P/l
                                   0.31  mg N/l
                                   870 g C/m /yr (phytoplankton
                                                  productivity)
                                   0 kg P/yr
                                   1200 kg P/yr
                                   0.9 g P/m2/yr

                                   0 kg N/yr
                                   7200 kg N/yr
                                   5.14 g N/m2/yr
                                   0.08  mg  P/l
                                   0.06  mg  P/l
                                   1.0 mg N/l
                                            2
                                   4.6  g C/m /day
                   Investigator-Indicated Comments
Lake has extensive littoral  zone  and  exhibits  large amount of
macrophyte growth.
                            (continued)
                                451

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          DATA SUMMARY FOR WINGRA (WISC.) - (continued)
 Does not include water body surface area.

 Data based on samples obtained at weekly intervals during
 1970-1971, at one and two meters, from four open lake and
 four littoral zone stations.

Dash (-) No data available.

 Data taken from Rast  and  Lee  (1975)  and  personal communication

 (Table  3).
                                452

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                             GLOSSARY



                              2
A,           Watershed area (L )
 a
                                       2
A            Water body surface area (L )



L            Areal loading (ML'2?'1)



L            Critical loading (ML'2?'1)



L (P)        Permissible ("critical") total phosphorus loading

             (ML-2T-1)

                                       -2 -1
L(N)         Total nitrogen loading (ML  T  )



L(P)         Total phosphorus loading (ML'2?'1)

                                                        _3
L(P)/q       Influent total phosphorus concentration (ML  )


                                                  -3 -1         -
£(P)         Volumnar total phosphorus loading (ML  T  ) = L(P)/z



MDR          Meteoric discharge rate (LT~ )

                                                       _ q
[P] S [P]X   In-lake total phosphorus concentration (ML  )

                                                        _ q
[P]          Influent total phosphorus concentration (ML  ) =

             L(P)/q
                   o
                                                      _ q
[P].         Inflow total phosphorus concentration (ML  )

                                                       _ q
[P]          Outflow total phosphorus concentration (ML  )



[P] P        Critical total phosphorus concentration at spring

             overturn (ML~3)


r-r.-, summer    „           •   •,  •>   ^ .  -,   -^   -.            ^   •
IP],         Summer mean in-lake total phosphorus concentration

   X         (ML-3)



[P],         Total phosphorus concentration at time t (ML~ )

                                                         _ o

[P],         Total phosphorus concentration at time 0 (ML  )

    o                                                       _3

[P]          Steady state total phosphorus concentration (ML~ )
                                453

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Q            Annual inflow or outflow volume (L )
                             3
q.           Inflow volume (L )
                              2
q            Outflow volume (L )
 o
q            Hydraulic loading (LT~ ) = Z/T  = areal water load
             (Q/Ao)

R            Retention coefficient

R   •         Nitrogen residence  time (T)

R            Phosphorus residence time (T)
 P
l-R(P)       Fraction of phosphorus input not retained in sediment

T            Phosphorus residence time (T)
                                 3
V            Water body volume (L )

v            Apparent settling velocity of total phosphorus
             (LT-1) = « v1

v.           Flow rate in j th tributary (L3T~1)

v-'-           Settling velocity of settleable particulate phosphor-
             us (LT-1)

z            Mean depth (L) = V/A
                    c            o
<*            Fraction of total phosphorus represented by settle-
             able particulate phosphorus

p            Flushing rate (T  )

p            Hydraulic flushing  rate (T~ )

TT            Phosphorus residence time relative to hydraulic
             residence time (TT~^) = f /T
                                      p  oo

a          '  Sedimentation rate  coefficient (T  )

a            Phosphorus sedimentation rate coefficient (T~ )

T            Hydraulic residence time (T)
                                 454

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                                   TECHNICAL REPORT DATA
                            (Please read Instructions on the reverse before -ompleting)
1. REPORT NO.
   EPA-6QO/3~78-nna
                                                           3. RECIPIENT'S ACCESSION NO.
4. TITLE AND SUBTITLE
  SUMMARY ANALYSIS OF THE NORTH AMERICAN (U.S. PORTION)
  OECD EUTROPHICATION PROJECT: Nutrient Loading—Lake
  Response  Relationships and Trophic  State Indices
                     DATE
                       1978
             6. PERF-,RM)NG ORGANIZATION CODE
7. AUTHOR(S)
 Walter  Rast  and G.  Fred Lee
             8. PERFORMIIG ORGANIZATION REPORT NO.
9. PERFORMING ORGANIZATION NAME AND ADDRESS
 Center for  Environmental Studies
 The University of Texas at Dallas
 Richardson,  TX  75Q80
             10. PROGRAM ELEME.T No

               1BA608
             11. CONTRACT/GRANT NO.~

               R-803356-01-0
               R-803356-01-3
12. SPONSORING AGENCY NAME AND ADDRESS
 Environmental  Research Laboratory  -  Corvallis, OR
 U.S. Environmental  Protection Agency
 200 SW  35th  St.,  Corvallis, OR  97330
             13. TYPE OF REPORT AND PERIOD
               Final -  July 1974/NovemU
             14. SPONSORING AGENCY CODE


                EPA/600/02
15. SUPPLEMENTARY NOTES
  Companion document to  EPA-6'00/3-77-086:NORTH AMERICAN PROJECT - A Study  of U.S.
                                           I'/ater Bodies
16. ABSTRACT
 This report  summarizes and critically  analyses nutrient load-lake response relation-
 ships for  38 U.S.  water bodies which have been intensively  studied by 20 scientists
 participating in  the OECD Eutrophication Program.

 It was determined  that the Vollenweider nutrient load relationship involving mean
 depth, hydraulic  residence time and phosphorus load correlated  well  with the trophic
 states assigned by the individual  investigators.

 A good correlation was also found  between phosphorus loading, normalized as to hydrau-
 lic residence time and mean depth, and the average chlorophyll  and water clarity  (as
 neasured by  Secchi depth).

 The relationships  developed in this study can be used to predict  the improvement  in
 water quality that will  result from a  change in the phospohrus  load  to a water body
 for which  phosphorus is the key chemical  element limiting planktonic algal  growth.
17.
                                KEY WORDS AND DOCUMENT ANALYSIS
                  DESCRIPTORS
                                              b.lDENTIFIERS/OPEN ENDED TERMS
                           c.  COSATI Field/Group
 eutrophication
 lakes
 nutrients
 phosphorus
 nitrogen
 loading
                              05B
                              05C
18. DISTRIBUTION STATEMENT

 Release unlimited
19. SECURITY CLASS (This Report)
 Unclassified
21. NO. OF PAGES

  478
20 SECURITY CLASS (This page)
                           22. PRICE
                                                Unclassified
EPA Form 2220-1 (Rev. 4-77)   PREVIOUS EDITION is OBSOLETE
                                             455
     rU S GOVERNMENT PRINTING OFFICE 1978—799-716/74 REGION 10

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