SECTION: TABLE OF CONTENTS
                                                       VERSION: FINAL/MAY  1997
                                                       CHAPTERS 1 through 9
                                  TABLE OF CONTENTS
ACRONYMS

CHAPTERS
1  - POLICY AND RESPONSIBILITY

1.1     Policy
       1.1.1 - Overview
       1.1.2 - Research Vessel Physical Characteristics
1.2     Purpose   •"-
1.3     Policy and Program Goals
1.4     Responsibilities
       1.4.1 - Assistant Regional Administrator
       1.4.2 - Regional Occupational Health & Safety Manager
       1.4.3 - Office Director
       1.4.4 - GLNPO Occupational Health & Safety Manager
       1.4.5 - Supervisors and Managers
       1.4.6 - Project Officers/Work Assign. Managers & Contract Officers
       1.4.7 - Contractors and Grantees
       1.4.8 - Chief Scientist
       1.4.9 - Shift  Supervisor
       1.4.10 - Employee Rights and Duties
       1.4.10.1  - Employee Compliance
       1.4.10.2 - Employee Rights and Responsibilities
       1.4.10.3 - Employee Conduct and Discipline
1.5     Safety Committee  Policy
1.6     Safety Committee  Organization
1.7     Committee Operations
1.8     GLNPO Safety Program Goals
1.9     Program Budget and Resources
1.10    Dissemination of Occupational Health & Safety Program Info.
1.11    Worker Concerns
       1.11.1 - Reporting
       1.11.2- Recordkeeping
1.12    Injury and Illness
       1.12.1 - Injury and Illness Definitions
       1.12.2 - Reporting Procedures
       1.12.2a - Ship Board Reporting Requirements
       1.12.3 - Investigation
       1.12.4 - Recordkeeping
1.13    Annual and Periodic  Inspections and Surveys
                                                           U.S. Environmental Protection Agency
                                                           Region 5, Library (PL-12J)
                                                           77 West Jackson Boulevard, 12th Floor
                                                           Chicago, IL  60604-3590

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                                                        SECTION: TABLE OF CONTENTS
                                                        VERSION: FINAL/MAY 1997
                                                        CHAPTERS  1 through 9
                            TABLE OF CONTENTS (Continued)

  CHAPTERS

  Attachments:

         1.1     Occupational Health & Safety for EPA Employees Poster
         1.2     The GLNPO Chief Scientist
         1.3     Incident Reporting Policy and Procedure

  2 - TRAINING, CERTIFICATION. AND MEDICAL MONITORING

  2.1    Required Training Policy
         2.1.1 - 24-Hour Laboratory Safety Course
         2.1.2 - 4-Hour Laboratory  Safety Refresher Course
         2.1.3 - GLNPO Research Vessel Laboratory Training Requirements
         2.1.4 - Radiation Safety Training
         2.1.5 - 40-Hour and 24-Hour Field Health  and Safety Training
         2.1.6 - 8-Hour Field Health and Safety Annual Refresher Training
         2.1,7 - Terrestrial Training
         2.1.8-  First Aid and CPR
         2.1.9 - Respiratory Protection
         2.1.10 - Emergency Response Team Training
•• 'V£2"':-'-''Safety Training r Ship Operating Personnel
       ?'- "2.211-' -Fire': Fighting
till ,l;; '2,2.2 -Cranes ;-;  : \
      l>-£-23''- Powered Industrial  Trucks (Fork Lifts)
  2.3    Documentation
  2.4    Medical Monitoring Selection Policy
  2.5    Additional Health and Safety Courses
         2.5.1 — Occupational Noise Exposure
         2.5.2 - Hazard Communication
         2.5.3 - Confined Space Entry
         2.5.4 - Hot Work Procedures
         2.5.5 - Lockout/Tagout
         2.5.6 - Bloodborne Pathogens
         2.5.7 - Personal Protective Equipment

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                                                    SECTION: TABLE OF CONTENTS
                                                    VERSION: FINAL/MAY 1997
                                                    CHAPTERS 1 through 9
                         TABLE OF CONTENTS (Continued)

CHAPTERS

Chapter 2 Attachments:

       2.1    24 Hour Laboratory Safety Training Agenda
       2.2    General Laboratory Safety Rules
       2.3    4 Hour Laboratory Safety Training Agenda
       2.4    Training Agenda for GLNPO Research Vessel Personnel
       2.5    Powered Industrial Trucks Rules of Operation


3 - COMPLIANCE REVIEW AND STANDARD OPERATING PROCEDURES

3.1     Overall Policy - (Applicable to GLNPO and Contractors)
3.2     Safety and Health Review of New Construction/Repairs and
       Alterations
3.3     Safety & Health Reviews of Marine Construction/Repairs and
       Alterations
3.4     Review of Hazardous Materials & Hazardous Waste Disposal
       Procurement
3.5     Health and Safety Review of Contracts
3.6     Review of Miscellaneous Procurement Impacting Safety,
       Health or Environmental Compliance
3.7     SOP Policy
3.8     Field SOP's - (ARCS)
3.9     Laboratory Chemical Hygiene SOP's
3.10    Marine SOP's
3.11    Evacuation Plan


4 - RESEARCH VESSEL LABORATORY HEALTH AND SAFETY
4.1     GLNPO Chemical Hygiene Plan
4.2     Safety Orientation Video
4.3     Boat Handling and Seamanship
       4.3.1 - Safe Boating Operations - Small and Large Vessels
       4.3.2 - Personal Flotation Devices
       4.3.3 - Man Overboard Procedures
       4.3.4 - Fire
       4.3.5 - Weather
       4.3.6 - Distress Signals

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                                                   SECTION: TABLE OF CONTENTS
                                                   VERSION: FINAL/MAY 1997
                                                   CHAPTERS 1  through 9
                         TABLE OF CONTENTS (Continued^

CHAPTER
      4.3.7 - Fueling
      4.3.8-Rules of the Road
4.4    Small Boat Operations
4.5    Large Boat Operations
Chapter 4 Attachments:

       4.1    R/V Lake Guardian Welcome Aboard



5 - PERSONAL PROTECTIVE EQUIPMENT

5.1     GLNPO Personal Protective Equipment                                                 4
5.2     Levels of Protection                                                                 *

Chapter 5 Attachments:

       5.1    GLNPO Personal Protective Clothing & Equipment Assignment
       5.2    Personal Protective Equipment Assessments


6 - MARINE SAFETY

6.1     Ship Operation Safety
6.2     Ship Personnel Safety
6.3     New Employee Orientation
6.4     Fire Safety
6.5     First Aid and CPR
6.6     Occupant Emergency Plan
6.7     Confined Space Entry
6.8     Visitor Safety

Chapter 6 Attachments:

       6.1    Visitor Safety

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                                                   SECTION: TABLE OF CONTENTS
                                                   VERSION:  FINAL/MAY 1997
                                                   CHAPTERS 1 through 9
                         TABLE OF CONTENTS (Continued)

CHAPTERS

7 - EMERGENCY PREPAREDNESS AND RESPONSE

7.1    Hazardous Material Spills- RCRA, OSHA
7.2    Hazardous Material Transportation
7.3    Fire Fighting Operations
7.4    Injury, Illness, or Medical Emergency
7.5    Contractor Support Operations
7.6    Evacuation Plan for Visitors During Open House
7.7    Security Aboard Research Vessels Du^ng Open House

Chapter 7 Attachments:

      7.1    Station Bill Checklist
      7.2    Firefighting Operational Diagram
      7.3    Pre-Open House Checklist
      7.4    Medical Advice Contractor
      7.5    Marine Medical Emergency Operations Plan


8 - ENVIRONMENTAL COMPLIANCE

8.1    TSCA-PCBs
8.2    RCRA
      8.2.1 - Solid (Non-Hazardous) Waste Handling, Storage, and Disposal
8.3    Drinking Water
8.4    GLNPO Environmental Compliance Manual
8.5    Air Emissions and Toxics
8.6    Indoor Air Quality
8.7    Asbestos Precautions
9 -HEALTH & SAFETY PROCEDURES DURING GLNPO SEDIMENT SAMPLING
OPERATIONS

9.1    Background
9.2    Safety
9.3    Storage of Sediment
9.4    Homogenization and Preparation of Elutriate Samples
9.5    Cleanup of Equipment after Sediment Processing
9.6    Sediment Assessment Field Operations Safety SOPs

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ACRONYMS
ACGIH

ANSI

APR

ARA

ARCS

CAA



CBC

CERCLA
CFR

CGI

Class I

Class II


CNS

CPR

CRC

CRZ

CWA
dBa
American Conference of Governmental Industrial Hygienists

American National Standards Institute

Air Purifying Respirator

Area Regional Manager

Assessment and Remediation of Contaminated Sediments

Clean Air Act:  Prevent or control the emission of any substances into the air that may
harm public health or natural resources; set standards to define acceptable tolerance
levels for air pollutants.  (40 OR 50-80)

Complete blood count

Comprehensive Environmental Response, Compensation, and Liability Act: Original
regulatory goal (1980) was focused on the cleanup of abandoned hazardous waste sites
nationwide. A  government trust fund (the "Superfund") pays for cleanups; EPA
identifies potentially responsible parties (PRPs) and orders them to clean up the site or
bills for cost of cleanup performed by the  government.  (40 CFR 300s)

Code of Federal Regulations

Combustible Gas Indicator

Materials with flash points below  100°F (see 29 CFR 1910.106)

Materials with flash points at or above 100°F, but below 140°F (see 29 CFR
1910.106)

Central nervous system

Cardiopulmonary Resuscitation

Contamination  Reduction Corridor

Contamination  Reduction Zone

Clean Water Act: Federal Water Pollution Control Act FWPCA. Restore and maintain
the integrity of surfaced waters in the United States by preventing or controlling
pollution, with the ultimate goal of making all surface waters usable for fishing and
swimming.  (40 CFR 110-140, 40 CFR 400-470)

Decibels (decibel measured on the A-scale)

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DOT          Department of Transportation

U. S. EPA     U.S. Environmental Protection Agency

EPCRA       Emergency Planning and Community Right-To-Know Act of 1986 (SARA Title III).
              Establish regulations for industry to report information regarding chemicals stored at
              facilities; establish a framework for emergency planning; establish reporting procedures
              for toxic chemical releases. (40 CFR 350, 355, 370, and 372)

EPIRB        Emergency Position Indicating Radio Beacon

FEF          Forced expiratory flow

FID          Flame ionization detector

FIFRA        Federal Insecticide, Fungicide, and Rodenticide Act.  Establish safety standards for
              pesticides; restrict the use of,  remove from the market, or refuse registration for
              products that do not need the  safety standards. Note that HFRA broadly defines
              "pesticides" to cover anything intended to kill, repel, or control any nonhuman form of
              life, from bacteria-killing disinfectants to herbicides for weed control.  (40 CFR 150-
              186)

FRC          Functional residual capacity

GC           Gas chromatography

GLNPO       Great Lakes National Program Office

GLWQA      Great Lakes Water Quality Agreement

HMTA       Hazardous Materials Transportation Act. Centralize federal regulations for domestic
              packaging, labeling, and shipping of materials that pose a risk to health, safety, or
              property. (49 CFR 170s)

IAG          Inter Agency Agreements

IDLH         Immediately dangerous to life or health

kg           kilogram

IR           Infrared

LEL          Lower explosive limit

LFL          Lower flammable limit

ME          Medical Emergency

MEFR        Maximal expiratory flow rate

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MSDS

MSHA

MVV

NFPA

NIOSH

OSC

OSH Act


OSHA

OVA

PAPR

PCB

PDS

PEL

PID

PMS

PPE

ppm

PSA
psi

PVC

RBC
Material Safety Data Sheet

Mine Safety and Health Administration

Maximal voluntary ventilation

National Fire Protection Association

National Institute for Occupational Safety and Health

On Scene Coordinator

Occupational Safety and Health Act. Assure the safety and health of workers; set
standards to reduce or prevent  illnesses and injuries among workers. (29 CFR 1910)

Occupational Safety and Health Administration

Organic vapor analyzer

Powered air-purifying respirator

Polychlorinated biphenyl

Personnel decontamination station

Permissible exposure limit

Photoionization detector

Program Management Staff

Personal protective clothing and equipment

Parts per million

Pipeline Safety Acts. Under DOT, set minimum safety standards for the design,
construction, and operation of pipelines carrying natural gas, liquefied gas, or
hazardous liquids. Standards also cover repair requirements and accident and leak
reporting requirements.  (49 CFR 190-195)

Pounds per square inch

Polyvinyl chloride

Red  blood count

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RCRA        Resource Conservation and Recover)' Act. Regulate the generation, transport, and
              ultimate disposal of all forms of hazardous waste and industrial by-products, ranging
              from open garbage dumps to buried chemicals. Drafted as a solid waste
              disposal/recycling law, actual implementation focuses heavily on identification and
              tracking of hazardous wastes.  Note that disposal under RCRA may trigger compliance
              duties under additional laws if land, air or water quality may be affected by the disposal
              method. (40 CFR 260-263 and 264-270)

REL          Recommended exposure limit

RPM          Remedial Programs Management

RPS          Remedial Programs Staff

RV           Residual volume

SAR          Supplied-air respirator

SCBA        Self-contained breathing apparatus

SDWA        Safe Drinking Water Act.  Establish uniform federal standards for drinking water
              quality, and set up a system to control underground injection (or burial) of wastes and
              other substances that could contaminate such underground water sources as municipal
              water systems, public wells, and sole-source aquifers.  (40 CFR 140-149)

SHEMD      Safety, Health, and Environmental Management Divisions

SOLAS       Safety Of Life At Sea

SOP          Standard Operating Procedure

SRS          Surveillance and Research Staff

TLC          Total  lung capacity

TLV          Threshold limit  value

TLV-C        Threshold limit  value - ceiling

TLV-STEL    Threshold limit  value - short-term exposure limit

TSCA        Toxic Substances Control  Act of  1976. Evaluate the health and environmental risks of
              all chemicals before manufacture  or use in the United States.  EPA gathers data by
              screening new and existing chemicals through industry reporting and recordkeeping,
              including testing for possible risks. (40 CFR 700-750, 40 CFR  760s and 40 CFR 790-
              799)
TSD
Treatment, Storage, and Disposal Facility

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TWA        Time-weighted average




UEL         Upper explosive limit




UFL         Upper flammable limit




USCG        U.S. Coast Guard




UV          T rltraviolet

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                                                         SECTION:  CHAPTER 1
                                                         VERSION:  FINAL/MAY  1997
                                                         PAGE:  1 of 13
Chapter 1 - POLICY AND RESPONSIBILITIES

1.1    POLICY

The Great Lakes National Program Office (GLNPO) is responsible for overall coordination of the U.S.
EPA's efforts  to  protect  and  enhance Great Lakes Water Quality and restore and maintain the
beneficial uses of the Great Lakes.  GLNPO's role was established through the Great Lakes Water
Quality Agreement (GLWQA)  with Canada and through the Clean Water Act which charges the U.S.
EPA through GLNPO for overseeing the implementation of the agreement. The direct authority and
responsibility, however, for implementing the CWA and other federal laws necessary to carry out the
GLWQA, is the primary responsibility of various headquarters program offices as well as Regions 2,
3, and 5, and the Great  Lakes  States.

GLNPO, a headquarters organization, is physically located within the Region 5 management structure.
Therefore, the  safety policies defined here are provided to  augment the Region 5 Safety Plan.  This
plan is necessary in light of the unique multi-regional  and multi-national programs of GLNPO which
involve operation of various surveillance and research vessels as well as field demonstration programs.
Any safety or compliance issue not covered here is subject to the Region 5 Safety Manual.
GLNPO HEALTH AND SAFETY POLICY STATEMENT

It is the policy of the US EPA, Great Lakes National Program  Office,  that all EPA employees
working in offices, laboratories, field activities, and special programs are entitled to a comprehensive
health and safety program. The goals of the program are to ensure that each employee is able to work
in an environment free of recognized hazards and to reduce and mitigate occupational accidents as
much as possible.  These goals shall be accomplished through:

       1.      Training employees in the proper techniques for field investigations, proper use of and
              access to safety equipment, and proper protocol for inspections;

       2.      Development of standard operating procedures  for routine and non-routine work
              environment

       3.      Enabling employees to identify those work situations where they believe a hazard
              exists without any adverse action taken against the employee;

       4.      Medical monitoring of employees in risk situations.

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By implementing these procedures it is the desire of the U.S. EPA to see that it can mutually share
the responsibility to reduce work place hazards and that the Agency can provide the environment for
that to occur.

This policy shall be coordinated  by the chair of the GLNPO Safety Committee in conjunction with
the Regional Safety Manager.  In  addition to the outlined procedures,  this policy shall meet the
applicable requirements of the Occupational Safety and Health Administration, the provisions of
Executive Order 12196 and  other  relevant statutes, laws, policies and guidance of the Federal
Government regarding occupational  health and  safety.

1.1.1   Overview

The Great Lakes National Program Office (GLNPO) maintains a trained staff that is actively involved
in laboratory,  field, and special  programs (e.g., Terrestrial and Habitat) operations.  In addition,
GLNPO maintains a fleet of surveillance vessels devoted to limnological research.  The fleet consists
of the Lake Guardian, Hydra, and Mudpuppy. The Lake Guardian is the primary research platform
for GLNPO.   Formerly an  offshore oil-field supply vessel, the R/V Lake Guardian is the newest,
largest, and most sophisticated vessel among the fleet.  Historically, water used aboard research vessels
for engine cooling, in showers, and in sinks was discharged  overboard. The Lake Guardian, however,
is a non-polluting vessel with no overboard  discharges.

The Hydra is currently in lay-up status and was the principle vessel used during the 1970s and  1980s
for sampling Lake Erie when the  lake was in its most devastated condition.  The  Mudpuppy,  a
significantly smaller vessel, is capable of fresh-water sampling excursions in and around rivers, creeks,
and streams which flow into the Great Lakes.  Additionally, warehouse facilities are conveniently
located for vessel maintenance and equipment storage.

In conjunction with the Central Regional Laboratory in Chicago, Illinois, the Lake Guardian provides
analytical  support to GLNPO.

1.1.2   Research Vessel Physical Characteristics

       a.   The R/V Lake Guardian

           Length - 180'                            Cruising Speed -  13 mph
           Beam - 40'                              Fuel Consumption - 75 GPH
           Draft - 11'                               Range - 9000 miles
           Berthing Capacity - 42                    Endurance - 15-30 days

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    b.      The R/V Hydra

           Length - 65,6'                          Cruising Speed - 9 mph
           Beam -  17.8'                           Displacement (loaded) 95 tons
           Draft - 6.6'                             Range - 500 miles
           Berthing Capacity - 8                    Endurance - 2 days

    c.      The R/V Mudpuppy

           Length - 33'
           Beam -s'
           Berthing Capacity - 0


1.2    PURPOSE

This manual establishes policies, responsibilities, procedures, rules, and guidelines for all GLNPO
Occupational Safety & Health and Environmental Compliance Programs. A copy of this manual and
the appropriate appendices must be readily available aboard our over-night research vessels.  The        ^
manual and appendices must also be available in GLNPO offices.                                       •

For operations performed on the R/V Mudpuppy, a health and safety manual, specific to  performing
sampling activities where personnel exposures to contaminants are either unknown or only suspected,
has been prepared.   Therefore, in addition to the comprehensive  GLNPO manual, a copy of the
September 17, 1996 Health and Safety Manual, Appendix N, must also be available on board the R/V
Mudpuppy.


1.3    POLICY AND PROGRAM GOALS

It  is the goal of GLNPO to administer its programs  in a manner that will assure that its employees
are free from recognized hazards.
1.4    RESPONSIBILITIES

The responsibilities of relevant EPA personnel are detailed in the following paragraphs, separated by
title.

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                                                         PAGE:  4 of 13
1.4.1   Assistant Regional Administrator

The  Assistant Regional  Administrator (ARA)  for the Resources  Management Division is the
Designated Regional  Occupational  Safety and  Health Official.   This ARA is  responsible for
establishing Regional Health and Safety and Environmental Compliance policies, programs, standards,
goals, objectives, and priorities.  The ARA is  also responsible for establishing  an organization
including the designation of a Regional Health and Safety Manager with an adequate budget and staff
to implement occupational health and  safety and environmental compliance programs at all levels
throughout Region 5 and  GLNPO.
1.4.2   Regional Occupational Health and Satety Manager

The Regional Occupational Health and Safety  Manager, under the direction of the ARA for the
Resources Management Division, is responsible for developing Regional occupational health and safety
and environmental compliance policies, programs, standards, goals, and objectives for evaluating the
effectiveness of the Region's occupational health and safety and environmental compliance programs
at all levels. He/she must also provide technical and management support, direction, and services to
all Regional occupational health and safety and environmental compliance programs. Consideration
for regulatory applicability will be ultimately determined by the Regional Occupational Health and
Safety Manager.
1.4.3   Office Director

The Director of GLNPO is responsible for implementing the Great Lakes National Program Offices
Occupational Health and Safety and Environmental Compliance Program and is accountable to the
Regional Administrator.
1.4.4.  GLNPO Occupational Health and Safety Manager

One Occupational Health and Safety Manager is appointed by the GLNPO Office Director.  This
manager serves as GLNPO's Health, Safety & Environmental Compliance Team Leader. The Team
Leader, working with Team Members who represent a complete cross section  of the Office staff,
comprises a safety team that is responsible for assisting GLNPO management in directing health and
safety  activities within the Office  and for coordinating such  activities with the Regional Safety
Manager.  The team meets on a regular basis (i.e., preferably monthly but at minimum quarterly).
Each month, the Team Leader reports to the Office management on significant issues.  Each year in
the first  quarter,  the safety team  presents their  proposed performance  agreement to the Office
management for approval.  The Office Safety Manager will also serve on the Regional Health and
Safety Committee.  (Safety Representatives may also be appointed at the Branch or staff level.)

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1.4.5   Supervisors and Managers

Supervisors and managers are responsible, to the extent of their authority, for providing employees
with working conditions free from recognized hazards that are likely to cause death or serious harm
(i.e., equipment,  place of employment, etc.).   Supervisors  and managers  shall comply with the
Region's Occupational Health and Safety and Environmental Compliance Standards, along with all
safety rules, regulations, and orders issued by the Region.  Furthermore, they are responsible for
enforcing these correct work practices.
1.4.6   Project Officers/Work Assignment Managers and Contract Officers

Project officers who manage support contracts and grants for sampling, analyses and boat operations
are responsible for  assuring that all GLNPO-funded activities adhere to the safety and compliance
requirements defined in this manual. This includes medical monitoring and all required  certification.
It is recommended that this requirement is defined in the scope of work for any GLNPO-funded grants
and contracts. Unresolved conflicts should be reported to the GLNPO Safety Manager for resolution.
1.4.7   Contractors and Grantees

All contractors and grantees that are involved in field activities on EPA vessels or equipment are
required to have read  the pertinent  contents of this  manual as well  as  adhere  to the specific
requirements.  A system should be implemented that assures these policies are met as a prerequisite
for boarding EPA vessels.

All contracts for contractors performing work for Region 5, or for any EPA  activity or organization
serviced by Regional Safety personnel, shall contain provisions, in writing, which require contractors
and contract personnel  to  follow, at  minimum, the same  rules  and requirements that U.S.  EPA
personnel follow while  performing similar work.  Wherever possible, contractual provisions should
require that all safety equipment and services (e.g. personal protective equipment, medical monitoring,
training, etc.) be equivalent to that required for EPA personnel in the Region.
 1.4.8   Chief Scientist

 All GLNPO vessels will have a designated "Chief Scientist" while undergoing EPA surveillance and
 research operations. The "Chief Scientist" role is defined in Attachment 1.2. The "Chief Scientist"
 should ensure that all survey activities are conducted safely and in compliance with the health and
 safety protocols of GLNPO.

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 1.4.9   Shift Supervisor

During 24-hour surveillance and research operations, GLNPO vessels will have a designated Shift
Supervisor.  The Shift Supervisor,  acting as an assistant to the Chief Scientist, is required to follow
many of the guidelines set forth for the Chief Scientists.  The Shift Supervisor must inform the Chief
Scientist of any situation which  could  compromise  health  and safety, sampling activities, or
scheduling.
 1.4.10  Employee Rights and Duties

EPA employees are expected to follow all health and safety rules and to perform duties in a manner
which will protect their own well being and that of their fellow employees.
 1.4.10.1  Employee Compliance

Employees are obliged to follow GLNPO's and the  Region's Occupational Health and Safety and
Environmental Compliance Standards, rules, regulations, and orders which are applicable to their own
job duties, actions, and conduct.  Employees  are also required to use safety equipment, personal
protective  clothing and equipment (PPE), and other  safety devices and, moreover, to follow safety
procedures that the Region deems necessary for their protection.
1.4.10.2  Employee Rights and Responsibilities

The rights  and responsibilities of EPA personnel with regard to occupational health and safety are
detailed in the Occupational Health and Safety for Environmental Protection Agency Employees
poster. (Attachment 1.1)
1.4.10.3  Employee Conduct and Discipline

It  is EPA policy that primary emphasis be  placed on preventing situations requiring disciplinary
actions through effective employee-management relations and that when work performance and/or
conduct are not maintained at acceptable levels, constructive corrective action be taken by responsible
supervisors and management on a timely basis.  It is critical that this policy is enforced due to the
unique contractor/contractee relationships and the extenuating circumstances caused by extensive field
work.

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Disciplinary action taken must be consistent with the precept of like penalties for like offenses, with
mitigating or aggravating circumstances taken into consideration. The action taken should be no more
severe than  sound judgement indicates is required to correct the situation and maintain discipline.
Disciplinary action may include informal written and verbal warnings and formal actions including
official written reprimand, suspension, reduction in grade, or removal from duty.  EPA Order 3120. IB
may be consulted in determining appropriateness of disciplinary actions.
1.5 SAFETY TEAM (COMMITTEE) POLICY

The GLNPO Occupational Health and Safety 1'eam (Committee) is the medium for achieving the
participation  of employees  in  the  GLNPO's  Occupational  Health and  Safety  Programs.   A
well-organized, balanced health and safety Team will  have the diversified knowledge of all job
operations and activities in GLNPO.  The Team is an advisory body, not a policy making group.
Committee {Team) members review data and render recommendations to aid management in making
appropriate policy decisions in the area of health and safety matters affecting employees. The GLNPO
Occupational Health and Safety Team shall:

       1.     Be effectively supported by management.                                               •

       2.     Be given specific tasks to accomplish, not general topics of consideration.

       3.     Include personnel from the program areas which committee  decisions will affect.

       4.     Provide effective  representation of all GLNPO employees.

       5.     Include a cross section of experts who have first-hand knowledge of pertinent work
              conditions and practices.

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1.6 SAFETY TEAM TCOMMITTEE) ORGANIZATION

Pursuant to  the  Safety Committee Bylaws and as specified in EPA Order  1440', the GLNPO
Occupational Health and Safety Team is similar to the Regional Health and Safety Committee. It is
a team that  is operational  in nature, consisting of representatives from  branches and staffs whose
recommendations it will affect.

The committee currently consists of the management advisor, who also serves as the Chief of the
Monitoring Indicators Branch and Technical Assistance & Analysis Reporting Branch of GLNPO, and
safety representatives who are appointed by their GLNPO staff or team leaders.  The number of
representatives is not strictly set, nor is the term for each member who serves on the  committee.
GLNPO consists of the following staffs and units:

       Office of the Director
       Data Integration Unit
       Program  Management Staff (PMS)
       Environmental Planning Staff (EPS)
       Remedial Programs Staff (RPS)
       Environmental Information Branch (EIB)
       Technical Assistance & Analysis Reporting Branch
       Monitoring Indicators Branch
       Policy Coordination & Communications Branch
       Region and GLNPO Team Leaders

The GLNPO Safety Manager will provide  organizational and technical guidelines for the committee
including providing an agenda for each meeting and recording and distributing the meeting's minutes.

These Safety Team  Members make up the overall operational  GLNPO  Safety Organization.  The
GLNPO Safety Organization is an operational group and is managed by the GLNPO Safety Manager.
Specific duties of Safety Representatives include coordinating safety  inspections and surveys, and
scheduling required training, respirator fit testing, medical monitoring exams, and related matters for
personnel  in their organization.  Safety Representatives may serve at the Office, Staff or Unit level
and assist  the Safety Manager in the overall management of the Health and Safety Program.

All Safety Representatives must report medical monitoring, required training, field certification,
respirator  fit testing, medical monitoring exams, and related matters to  the GLNPO Safety Manager.
These reports should be prepared using the designated forms and are to be reported to the GLNPO
Safety Manager during the  quarterly safety committee meeting. Additionally, Safety Representatives
are expected to assist their Division Management in reporting abatement status of health and safety
hazards  or deficiencies found and cited during Annual and Semi-Annual Occupational  Health and
Safety and Environmental  Compliance Surveys and Inspections,
1  See Appendix A. Note: This document applies to all federal employees and must be available at all federal
   facilities for review (e.g. The Rj'V Lake Guardian).

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1.7 COMMITTEE OPERATIONS

The GLNPO Occupational Health and Safety Team meets every three months, usually on the first
Tuesday of the second month of each quarter of the federal fiscal year (November, February, May,
August).  Issues and subjects discussed at committee meetings include:  Vessel Operations, Annual
Safety Inspection and Industrial Hygiene Survey results, the Medical Monitoring Program, the Annual
Safety  Budget, the Radiation Protection Program, the  Lab Safety  Program, Personal Protective
Equipment  (PPE), Safety Training Program,  and other organizational and technical issues affecting
GLNPO personnel.

The Regional Safety Committee Bylaws, adopted in June 1988, are currently in effect and govern all
activities of the GLNPO Safety (Team).
1.8 GLNPO SAFETY PROGRAM GOALS

The GLNPO  Safety Manager is responsible for preparing an annual work plan for submittal to the
GLNPO Office Director and the Regional Safety Manager.  The work plan should outline Regional
Safety, Health, and Environmental Compliance Program goals for the coming fiscal year and their
proposed method of implementation.  Time frames for implementation should also be included.  The
goals and their implementation should be proactive and designed to build and  improve the Regions'
proactive Safety, Health, and Environmental Compliance Programs.
1.9 PROGRAM BUDGET AND RESOURCES

The GLNPO Safety Manager shall ensure that all Safety, Health, and Environmental Compliance
submissions include proposed funding and resources for implementation. He/she, in conjunction with
the Regional Safety Manager, will effectively administer the GLNPO Occupational Safety, Health, and
Environmental Compliance Programs for all Offices, Staffs and Units in GLNPO.

Appropriate funds and other resources for administering the programs shall provide for, but not be
limited to:

        1.      Sufficient personnel resources (including contract  resources)  to  implement and
               administer the overall program at all levels;

        2.      Necessary administrative costs for  such services as training,  personal protective
               equipment, etc.;

        3.      Contracts and lAGs for  medical monitoring, complete hygiene, and environmental
               compliance  services including laboratory analysis and hazardous waste  disposal
               services;

        4.      Safety and  health sampling,  testing, and diagnostic and  analytical supplies and
               equipment;

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       5.      Program training and promotion materials such as posters, slides, films, and video
               tapes, and;

       6.      Technical information, documents, books, standards, codes, and publications.
Most of these services will be provided through the Regional Health and Safety Manager to avoid
duplication of effort. Appropriate contracts and grant funds will be provided to the Regional Health
and Safety Manager for provision of these services.
1.10   DISSEMINATION  OF  OCCUPATIONAL HEALTH  AND  SAFETY PROGRAM
       INFORMATION

The GLNPO Safety Manager shall promote employee awareness of Occupational Safety, Health, and
Environmental Compliance Programs by:

       1.      Providing, upon request, copies of Executive Order 12196, 29 CFR 1910, and the
               Region's Occupational Health and Safety Program to all employees.  Also, copies of
               Agency and Regional standards and other applicable occupational health and safety
               standards shall be available to all employees for review upon request.

       2.      Posting a copy  of the Agency's  Policy poster in a conspicuous location in each
               Agency facility.

       3.      Bringing Occupational Health and  Safety issues to the attention of employees through
               in-house publications, fonims, and promotional materials.
1.11 WORKER'S CONCERNS

If an employee considers a  situation or a work condition  to be  a safety hazard,  it is his/her
responsibility to report it directly to the Safety Manager or his/her staff/unit or office Safety Designee.
It is an employee's responsibility to report potential safety hazards. (No one can take action against
an individual who reports a safety problem.)

1.11.1  Reporting

Potential safety hazards and incidents should be reported in writing to the GLNPO Health and Safety
Manager as soon as they are recognized.  The GLNPO Health and Safety Manager, in conjunction
with the Regional Health  and Safety Manger, and  his/her  staff will investigate the situation and
recommend corrective actions, if  necessary.  The Regional Health and Safety Office will either make
these corrections or pass along their recommendations to a more appropriate party, i.e. the General
Services  Administration, building management, etc.  The  GLNPO reporting  procedure, entitled
Incident Reporting Policy & Procedure, is provided in Attachment 1.3.

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                                                          SECTION:  CHAPTER 1
                                                          VERSION: FINAL/MAY 1997
                                                          PAGE:  11 of 13
1.11.2  Recordkeeping

Copies of all employee complaints, as well as documentation of corrective actions, are kept on file
in the Regional Health and Safety Office.

1.12   INJURY & ILLNESS

When an EPA employee suffers an injury, whether traumatic or non-traumatic, or is stricken ill while
on the job, there are necessary procedures to be followed in order to protect the employee's interests
and well being.  These procedures are defined in the following Sections 1.12.1  and 1.12.2.

1.12.1  Injury and illness Definitions

Injuries are considered either "traumatic" or "non-traumatic."  A "traumatic  injury" is defined as a
wound or other condition of the body caused by an external force, including stress or strain. A "non-
traumatic injury," also referred to as an "occupational disease or illness," is a condition  caused or
aggravated by working in a job environment over an extended period.  This includes injuries and
illnesses  caused by repeated  stress or strain, systemic infection, and those caused by continuous or
repeated  exposure to toxins, poisons, fumes, etc.

1.12.2  Reporting Procedures

Employees must report all accidents or incidents, which may result in an injury, to their immediate
supervisor and may do so without fear of reprisal.  Moreover, it is in the employee's best interest to
report all injuries sustained on the job, regardless of how minor they  may appear,  because a slight
injury could develop into something more serious.

Specific  forms  are used for reporting an injury.  When reporting a traumatic  injury, complete Form
CA-1,  "Federal  Employee's Notice  of Traumatic Injury  and  Claim  for Continuation  of
Pay/Compensation."   When  reporting  a non-traumatic injury, complete  Form  CA-2, "Federal
Employee's Notice of Occupational Disease and Claim for Compensation." (These forms are available
through the Human Resources Department.)  If an employee is incapacitated and unable to complete
these forms, another person (co-worker,  supervisor, etc.)  may complete them.  All forms  should be
signed by this other person and forwarded to the employee's supervisor.

The following steps should be taken consecutively by an employee in the event of a traumatic injury:

        1.      Report the injury or incident to your supervisor as soon as possible.

       2.      Obtain medical attention. Emergency treatment does not require prior authorization.
               In case of non-emergency treatment,  your  supervisor  must first complete a CA-16
               form (within four hours of  an employee's request)  in order to authorize medical
               treatment. (This form is valid for 60 days from date of issue unless canceled by the
               OWCP.)  Choose a  local federal  medical  officer/hospital,  if available, or a local
               private physician/hospital, and schedule a consultation.  Bring the CA-16 form and
               form HCFA 1500 (standard billing form) with you to  your appointment.

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       3.      File an official written notice of a traumatic injury within two working days, using
               form CA-1. (Continuation of Pay cannot be authorized if this form is filed more than
               30 days after the date of injury.)  Form CA-2 is filed  instead of  form CA-1 if
               disability results  from an  occupational disease or illness.

       4.      Retain a receipt of Notice of Injury from your supervisor for your personal records.
               It is attached to Forms CA-1 and CA-2.

       5.      If disabled as a result of a traumatic injury, the employee may choose to take leave
               or  request Continuation of Pay (maximum of 45 calendar days.) If disability is a
               result of an occupational disease, an employee may use leave or request compensation
               using Form CA-7.

Supervisors shall investigate and  report all job-related accidents on EPA Form 1440-9 "Supervisor's
Report of Accident".  This is a four-part  form.  The supervisor who completes the form must mail
the top copy directly to the Environmental Protection Agency, Occupational Health abd Safety Office,
Washington, D.C.  20460.  If the accident involves an occupational  injury or illness,  the supervisor
must attach a copy of Form CA-1 or CA-2.  The fourth  part of the completed Form 1440-9 must be
retained by the supervisor. The supervisor must send the second part of the Form  1440-9 to the local
personnel office and the third part cf the  form to the Regional Safety Manager.

Supervisors should perform the following when summarizing an injury or illness:

       1.      Complete and forward all forms to the appropriate OWCP District Office within 10
               working days following the receipt from the employee if:

               •  The disease or illness causes disability for work beyond the  day or shift it was
                  reported; or

               •  It appears the condition will result in prolonged treatment, permanent disability,
                  or serious disfigurement of the head, face, or neck; or

               •  The condition has resulted (or will likely result)  in a charge for medical or other
                  related expenses.

       2.      Furnish a separate, narrative  statement to be attached to the form, which should:

               •  Describe in detail the work performed by the employee, identify fumes, chemical
                  or other irritants or situations that the employee was  exposed to which allegedly
                  caused the condition.  State the nature, extent and duration of exposure, including
                  hours per day and days per week.

               •  Attach copies of all  physical examination reports  (including X-ray reports and
                   laboratory data) on file for the employees.

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              •   Attach a record  of the employees  absence from  work caused by any similar
                  disease or illness (have employee state reason for each absence).

              •   Attach statements from each co-worker who has first-hand knowledge about the
                  employee's  condition and  its cause (the  co-worker should state how such
                  knowledge was obtained.

              •   Review  and  comment on the accuracy of the employee's statement.

       NOTE: For the employee's own protection, copies of all forms and
       documents should be made for his/her personal  files.
1.12.3 Ship Board Reporting Requirements

Any accident or incident, whether involving  EPA  personnel,  Contracting personnel,  or Grantee,
occurring during surveillance and research activities must be immediately reported to the Captain and
the ship's Safety Officer. An Incident Report  detailing the events of the incident must be provided
in writing to the Regional Safety Manager and  the GLNPO Director within 48-hours of the incident.

1.12.4  Investigation                                                                                ^

Investigation of an accident or illness is necessary to prevent similar occurrences. It is not designed
to establish guilt or innocence, but is an attempt to identify and abate potential hazards.  Investigations
should be conducted promptly by both the employee's supervisor and the  Occupational Health and
Safety  Designee.  Both parties should gather and document all facts and statements concerning an
incident,  make recommendations for resolution, and provide each other with copies of findings, as
well as to the employee involved. Further details of investigative procedures are available in EPA
Order 1440, Occupational Health and Safety Manual.

1.12.5  Rccordkeeping

Under OSHA regulations, it is the responsibility of the Safety Office to maintain and keep current two
OSHA Forms.  "The Log of Federal Occupational Injuries and Illnesses" is used for compiling data
and statistics on injuries and illnesses.  "The Annual Summary of Federal Occupational Injuries and
Illnesses" is a questionnaire completed by each Region for Headquarters in order to  assist them in
preparing the annual health and safety summary of the Agency for submission to the Department of
Labor. This log and information will be kept  at the Regional Safety Office.

1.13 ANNUAL AND PERIODIC INSPECTIONS AND SURVEYS

A summary of required inspections, frequency of inspections, and personnel responsible for specific
inspections for  all GLNPO operations is stated in Appendix V.

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                                                 SECTION: CHAPTER 1, ATTACHMENT I.I
                                                 VERSION: FINAL/MARCH 1997
                                                 PAGE:  Att. 1-1
Occupational
Safety  and Health
for Environmental
Protection Agency
Employees
  Occupational Satety and health Policy

  Tha Environmental Prolectlqn Agency shall administer its programs in a
  manner that will assure Its employees places and conditions of employ-
  ment free from recognized hoards which are likely lo cause death or
  serious harm.
Agency
Responsibilities
Do signals en Occupational Safety and
Health Official lo administer EPA't Safely
and Health Programs.  This Offfdaf I*

Sallyanne Harrer	
Nona

Acting Assistant Arhi./OAEM
Title

• Furnish employment and a place of
employment tree horn  recognized hazards.

•  Comply with OSHA standards or develop
more stringent alternate standards.

•  Ensure thai the performance evaluation
of at managers and supervisors measures
his/her performance in meeting requirements
of EPA'* Occupational Safety and Health
Program*.

* Acquire, maintain, and require the use of
approved personal protective equipment and
approved safely equipment

•  Authorize Agency safety and haalth
personnel to utilize expertise Irom other
agencies, professional groups, consultants,
universities, labor organizations, safety and
health committees, and other appropriate
sources.

•  Ensure appropriate resources to
effectively implement and administer the
Agency's Occupational Safety and Heallh
Programs.
 Occupational
 Safety and Health
 Responsibilities

 • Develop an Agency Occupational Safety
 and Heallh Program that includes policies,
 programs, standards, goals, and objectives
 * Evaluate '.he alleoveress 3! ;he
 Agency's Occupational Sa/ery and Health
 programs at all operating 'evals
Supervisor
Responsibilities
To the extent of their authority, Superior*
shal:
•   Provide hlsAer employees with    ,
employment and places of amployrm»-t ree
Irom recognized hazard*.

•   Comply with all safety and health
standards and with all rules, regulation; ind
orders Issued by the Agency.

•   Enforce safe work practices.
Employee
Responsibilities
•   Comply with Ihe Agency'* Occupaih nal
Safety and Health standards, rules,
regulations, and orders applicable to Ihni
own action* and conduct

•   Use Ihe safely equipment, persons!
protective equipment and other safety anil
health device* provided by *ie Agency.

•   Follow the procedures, provided or a;
directed, that the Agency deem* necesi a y
for their protection.                <  \

•   Report al work-related property and
personal accidents, and ilnesses to (her
supervisor.
 Employee and Employes
 Representative Rights
 •   Access to copies ol the Agency's
 standards, injury and iftness statistics, ard
 procedures.

 •   Comment on standards proposed b/
 the Agency

 •   Participate or assist in insoecticns an'
 tell inspectors about unsale or unrmalthlil
 working conditions.

 •   Authonzed ollicial lime 'o participate n
 :he Agency'3 Occupational Safety and
 Health Program activities

 •   May decline to perform assigned tas 9 Occupational Safety and r-eallh
 C^s ;-99

 J;j..jS  C.  J:Teno.  Dir.,SH5>'D
                                                                                                               9  1996

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                                                     SECTION: CHAPTER 1, ATTACHMENT 1.2
                                                     VERSION: FINAL/MAY 1997
                                                     PAGE: 1 of 2
Attachment 1.2 - THE GLNPO CHIEF SCIENTIST

The duties and responsibilities statement establishes standards for performance a: a Chief Scientist
(CS) against which Chief Scientists can be evaluated.   These are classified into two categories;
scientific/technical and managerial.

Duties and Responsibilities

Chief Scientists will  be expected to perform  a variety of duties and fulfill  specific obligations as
technical leaders of the survey party.

Scientific/Technical  Responsibilities:   Scientific/technical  responsibilities  include oversight  of
sampling activities.  In particular, Chief Scientists will be expected to:

               •       Coordinate  activities  with  the  vessel's  captain  regarding  sampling
                       schedules, and special needs or precautions for specific types of samples;

               •       Resolve conflicts as they arise; and

               •       Monitor sampling activities so that all samples are collected;

               •       Make final decisions regarding sampling priorities when time and adverse
                       weather conditions become limiting.

Managerial Responsibilities:  Managerial responsibilities include providing leadership, oversight of
project  activities,  communication   with  crew,  and  compliance  with  safety  requirements.    In
particular, Chief Scientists will be expected to:

               •       Assure all necessary equipment is acquired and is in good condition prior
                       to survey departure;

                       Maintain morale and encourage teamwork  during surveys;

                       Assign duties and monitor progress of work activities;

               •       Assure that a log of survey activities is  maintained (as appropriate);

               •       Ensure that all survey activities  are conducted  safely and in  compliance
                       with  health and safety protocols; and

               •       Support public outreach activities.

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                                                  SECTION: CHAPTER 1, ATTACHMENT 1.2
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Requirements and Stages of Practicum Progress

The  candidate will be  required to  complete  a minimum of three surveys aboard an EPA vessel.
The  individual will assume increasing responsibility over time, with approval from the mentor.

Specifically, the stages of practicum progress are:

       •       Participating in a survey as a survey party member,

       •       Serving as Watch Captain on a survey, and

       •       Serving as Apprentice Chief Scientist  during  a survey under supervision  by a
               certified Chief Scientist.

The  Chief Scientist will be expected to demonstrate knowledge and understanding of surveys.  The
Chief  Scientist  should  also be trained in  CPR and First  Aid and  act as a  first responder  in          *
emergency situations unless another designated person (e.g., Captain, Paramedic, EMT,  etc.)  is         •
available.

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                                                   SECTION: CHAPTER 1, ATTACHMENT 1.3
                                                   VERSION: FINAL/MAY 1997
                                                   PAGE: 1 of 1
Attachment 1.3 - INCIDENT REPORTING POLICY & PROCEDURE

An incident is defined as an event or an occurrence,  minor or otherwise, that  disrupts normal
procedure or causes a crisis.  In regards to health and safety, the term incident is defined to set a
basic guideline for employees to consider when faced with situations which may be considered an
unsafe and/or unhealthful condition.  Often it  is a combination of minor events/incidents which
lead to a  major  and more serious event/incident.  Therefore,  it  is imperative that  thoughtful
consideration and thorough investigation be used when faced with an incident.

POLICY

In accordance with EPA RS 1440, employees are responsible for detecting and promptly reporting
work hazards, risks, or any unsafe conditions noted in  their work environment.   If an  employee
considers a situation or work condition to  be a hazard,  or  has the potential to be a hazard, or is
faced with  an incident  which  may  lead  to  an  unsafe or unhealthful  condition, it  is  his/her
responsibility  to report  the potential safety hazards to  their supervisor or directly  to the Safety
Manager or his/her Division Safety Designee.  It is the responsibility of EPA  management for
promptly correcting these reported conditions.

REPORTING

Potential safety hazards or incidents should be reported in writing to the Regional Health & Safety
Office  as  soon as they  are recognized.   The Health &  Safety Manager  or his/her  designee will
investigate the situation  and  recommend  corrective action,  if necessary.  The Health & Safety
Office  will either make these correction or will pass the recommendations to a more appropriate
party.

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                                                          SECTION: CHAPTER 2
                                                          VERSION: FINAL/MAY 1997
                                                          PAGE: 1 of 9
Chapter 2 - TRAINING, CERTIFICATION, AND MEDICAL MONITORING

REQUIRED TRAINING POLICY - OVERVIEW

It is the policy of the Great Lakes National Program Office that all EPA personnel and research vessel
occupants, including ship contractors, laboratory contractors, and visiting scientists, have appropriate safety
training specific to their job functions  in accordance with EPA requirements.  All required courses will
be provided by the EPA Safety Manager, with the exception of Superfund training.  (Superfund training
will be provided by the Office of Superfund.) Training courses will be offered on an annual basis.

GLNPO training courses encompass a wide range of criteria including laboratory safety training, radiation
safety training, field health and  safety, first aid a^J CPR,  respiratory protection training, emergency
response, fire fighting,  crane operation, industrial truck operation,  occupational noise exposure, hazard
communication, confined space entry, hot work and lockout/tagout procedures, as well as bloodborne
pathogen training.  In addition to training courses, GLNPO adheres to the regional  medical monitoring
selection policy followed  by all EPA Region 5 employees.


2.1. REQUIRED TRAINING POLICY

All EPA and Contractor personnel who  work  in EPA owned  or  leased laboratory space (including
laboratories on research  vessels, and  all  field locations)  must meet  the safety training  requirements
specified in EPA Order 1440 and Regional Safety Manuals. These Regional Safety Manuals must be
readily accessible at all locations.  Supervisors must ensure that their employees are aware of these
manuals and that the requirements within the manuals will be implemented. Chapter Eight of EPA Order
1440 specifically addresses working with toxic substances in laboratories.  Regional and laboratory safety
regulations are designed to  comply  with the OSHA regulations  29 CFR Part  1910 "Occupational
Exposures to Hazardous Chemicals in Laboratories".  All laboratories are required to have a site-specific
safety manual. The manual should include general laboratory safety rules, fire escape plans, emergency
plans, environmental compliance rules, and a chemical hygiene plan.  All laboratory personnel must
receive training based on  the content of the manual and must know its location for reference purposes.

2.1.1  24-Hour Laboratory Safety Course

As  specified in Order 1440, Chapter Eight, a 24-Hour Laboratory Safety Course must be completed by
all EPA and contractor personnel  prior to working in an EPA laboratory on a full-time or part-time basis.
The 24-hour  Laboratory  Safety  Course is  designed to cover all  aspects of laboratory safety.   New
employees must  receive  on-the-job   training  including fire  evacuation  procedures, waste disposal
procedures, protective equipment requirements, the  location of eyewash stations, fire alarms, spill control
stations, and any site-specific  safety rules.  Topics  such as  potential  sources  of exposure, adverse health
effects, work  practices and engineering controls, environmental and medical  monitoring procedures, and
storage of incompatible materials should be covered, as well.  Additionally, since all laboratories generate
wastes, and some of theses wastes are hazardous under the RCRA regulations, the 24-hour training must
include information regarding environmental compliance. This will ensure that laboratory personnel will
understand compliance  according to RCRA regulations and how it relates to their work practices.  For

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                                                           SECTION: CHAPTER 2
                                                           VERSION: FINAL/MAY 1997
                                                           PAGE: 2 of 9
 further information, please refer to EPA Headquarters Order 3500.1 entitled "Training and Development
for Compliance Inspectors/Field Investigators".  In the event a new employee begins work in a laboratory
 prior to scheduled training, the employee should review the materials provided for the Laboratory Safety
 Class and refresher class.  The employee should then be enrolled in the first available Laboratory Safety
 Class.

 A training agenda for the 24-hour course can be found as Attachment 2.1 of this chapter.

 "General Laboratory Safety Rules" appear as Attachment 2.2 of this chapter.

 2.1.2  4-Hour Laboratory Safety Refresher Course

 Following the completion of the 24-hour Laboratory Safety Course, an annual refresher course is required
 thereafter.   The  4-Hour  Laboratory Safety A.^iual Refresher Course  is mandatory  for all EPA and
 contractor personnel who  work in EPA laboratories and is designed to review safety topics for personnel
 who  already have a basic understanding of safety requirements.   Recent advances in  health and safety
 regulations should be  included  during this refresher training.  The 4-Hour Laboratory  Safety Annual
 Refresher Course agenda  appears as Attachment 2.3 of this chapter.

 2.1.3   GLNPQ  Research,Vessel Laboratory Training Requirements

 In  addition to the 24-hour  Laboratory  Safety  Course and the annual 4-hour Refresher Course, all
 laboratory personnel are required to attend a "site-specific" training orientation course prior to  engaging
 in laboratory activities. This course is intended to familiarize EPA personnel and contractors with the
 laboratories in which they will work.   An Annual Refresher Course is required for this training and
 participants are required to document attendance.  The general training agenda for the course appears as
 Attachment 2.4  of this chapter.

 2.1.4  Radiation  Safety Training

 The U.S. Nuclear Regulatory Commission's Material License issued to the U. S. EPA Central  Regional
 Laboratory (CRL) allows the  limited  use of radioactive materials aboard  GLNPO  research vessel
 laboratories. This license also  allows GLNPO laboratories to use Carbon 14 and Nickel for laboratory
 research and sample analysis. While these materials may be used on a limited basis, Radiological Health
 and Safety Plans, in accordance  with the  U.S. Nuclear Regulatory Commission (NRC) Regulatory Guide
 8.8 and DOE publication  PNL-6577, will be implemented  to maintain the lowest minimum exposure to
 personnel.  Use of these  materials are  subject to the guidelines specified in the CRL Radiation Safety
 Manual as described in Appendix J of this manual.  GLNPO research vessels must also follow the
 guidelines presented in the Chemical Hygiene Plan described in Appendix J of this manual.

 A site-specific Radiation Safety Training Course is required for GLNPO  personnel and contractors prior
 to working in a GLNPO facility where radioactive materials are used.  This training must provide
 information on work operations using radioactive materials, personnel monitoring, protective clothing, and
 bioassay.   These training requirements  appear as part of  the Chemical Hygiene  Plan described  in
 Appendix J of this manual and radiation safety SOPs appear as an attachment to the plan.

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                                                          PAGE: 3 of 9                              '


2.1.5  40-Hour and 24-Hour Field Health and Safety Training

Health and Safety training is required for all EPA field personnel.  The training consists of a 40-hour
Superfund Hazardous Waste Site Training Course for all OSCs, RPMs, and related personnel.  All other
field personnel, including inspectors, are required to take the EPA 24-hour Field Safety Training Course.
All training  requirements will be in accordance with EPA Order  1440.2.

Since specific protection cannot be engineered into every field situation, it is essential that employees are
trained to implement safe  operational procedures and are familiar with the proper use of personal
protective clothing and equipment.   GLNPO employees  and contractors,  therefore, must successfully
complete the 24-hour or 40-hour training required and hold certifications attesting that these requirements
have been met. GLNPO employees and contractors are not  permitted to engage in routine field activities
until they have been trained  and certified.

No persons, field EPA employees, or contractors will be permitted to participate in GLNPO-sponsored
activities until they have received appropriate training .

2.1.6  8-Hour Field Health and Safety Annual Refresher Training

After certification in the 40-hour or 24-hour Field Health and  Safety Training Courses, all GLNPO
employees and contractor personnel are required  to complete the 8-hour Refresher Training Course on an     M
annual basis.  The refresher instruction is designed as a review of subject areas necessary to maintain     ™
certification based on the initial 40-hour and 24-hour training.

For further details on this subject, refer to EPA  Order 1440.2 Transrnittal July 12,  1981.

2.1.7  Terrestrial  Training

GLNPO  personnel involved  in Terrestrial operations are required to receive training in accordance with
the EPA 24-hour Field Safety Training Course specifications noted above in Section 2.1.5.

2.1.8  First Aid and CPR

EPA employees classified as Chief Scientist and  shift supervisors, and contractor personnel who work on
board GLNPO vessels are required to be certified in basic First Aid/CPR.

CPR
Courses in Cardiopulmonary Resuscitation (CPR) will be offered throughout the year.  Training may be
given by fellow  employees who are certified  instructors or by instructors from an  outside agency.
Assuming that someone  else will know  CPR is a dangerous assumption.  All EPA personnel aboard
GLNPO  vessels are required to attend an annual 4-hour course in CPR.

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                                                          SECTION: CHAPTER 2
                                                          VERSION: FINAL/MAY 1997
                                                          PAGE: 4 of 9
First Aid
First Aid, like CPR, will be taught throughout the year.  This course is valuable to all, but especially
important to personnel who are engaged in potentially hazardous activities. Like CPR, knowledge of First
Aid is valuable outside the work place, as well. Chief Scientists and Shift Supervisors who are required
to work on GLNPO research vessels must attend a 4-hour training session in First Aid, at minimum, once
every three years.
2.1.9  Respiratory Protection

Employees performing tasks requiring the use of respiratory protective devices must receive training in
the use and limitations of a respirator.  Personnel must also be evaluated by a physician to ensure they
are medically fit to wear a respirator. If respirators are issued, a written Respiratory Protection Program
must be implemented. Training and program requirements can be found in OSHA 29 CFR 1910.134 and
EPA Order 1440.3.

A written Respiratory Protection Program for GLNPO research vessels is described in Appendix A of this
manual. All Emergency Response Personnel aboard the vessel must meet the requirements specified in
the program.
2.1.10 Emergency Response Team Training

Prior to each GLNPO research vessel survey,  it is necessary to have a designated emergency response
team. The team must be trained in spill response, respiratory protection (including SCBA), and emergency
response in accordance with OSHA 1910.120.  The team members must also be trained in CPR and first
aid and conduct practice drills on an annual basis.

In the event anyone aboard a ship at sea becomes ill or is injured beyond the clear capability of the ship
to provide  prompt and adequate care, a Medical  Emergency  (ME) exists and that  person  should be
transferred  to a facility providing emergency medical treatment. Details and procedures for injured or ill
personnel or Medical Emergencies can be found in Chapter 7, Section 4.
2.2 SAFETY TRAINING FOR SHIP OPERATING PERSONNEL

Ship operating personnel must receive training  specific to their job functions in accordance with all
regulatory and policy requirements.   Specific  training  areas such as fire fighting,  heavy equipment
operation, and powered industrial truck operation, are detailed in the following sections.

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                                                           SECTION: CHAPTER 2
                                                           VERSION: FINAL/MAY 1997                ,
                                                           PAGE: 5 of 9


2.2.1  Fire Fighting

In the event of fire, a well-trained  fire  brigade  must be available to respond immediately  and be
accompanied by a qualified chief in command. All members of the GLNPO research vessel fire  brigade
must be trained in accordance with the U.S. Coast Guard's fire brigade requirements.  Training must
include discussions of fire principles  and  fire classification, the use of portable fire  extinguishers and
proper extinguishing methods, in addition to overall fire safety awareness.  Other qualifications of the
brigade must include training  and experience in  fire protection and prevention  in  addition  to well
established firefighting organizational capabilities.

Response teams must be available 24 hours a day and regularly scheduled training for all members of the
team must be conducted. Training sessions, at least one hour per week, are recommended. These sessions
should include proper procedures on how to av^d fire, hands-on training for drills, including signals and
individual responsibilities, as well as procedures for the prevention of bodily  injury.  A portion of this
training must be devoted to the  practice of emergency exit drills to make certain that all exits are clearly
marked and easily passable and that all alarms are in maximum working order.  Any deficiencies noted
during drills or routine inspections must be corrected immediately.

Training documentation must be made available aboard the vessel and must include the date of training,
name and social security number of the individual trained, and the name and qualifications of the trainer.


2.2.2  Cranes

Persons responsible for the operation of cranes,  derricks, or hoists on all GLNPO research vessels are
required  to receive  annual  training in  the  proper  use of Ship-Mounted  Load Handling Equipment.
Training documentation must be made available aboard the vessel and must include the date of training,
name and social security number of the individual trained, and the name and qualifications of the trainer.


2.2.3  Powered Industrial  Trucks (Fork Lifts)

The operator is one of the most important factors in the safe operation of industrial or fork-lift trucks.
To ensure operator competence, and mental and physical fitness, training is required.  Initial training must
be performed in accordance with the National Safety Council Guidelines followed by refresher  courses
every two years.  Training must include:

            Operator safety rules
        •    Basic material handling techniques
        •    Basic instruction on the operation of the truck
        •    A driver's performance test on an obstacle course which simulates actual driving conditions
            (e.g. maneuverability)

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                                                          SECTION: CHAPTER 2
                                                          VERSION: FINAL/MAY  1997
                                                          PAGE: 6 of 9
The operator should be trained to distinguish the differences between a fork-lift truck and a conventional
car or truck.  For example:

       •  A lift truck can be steered by either the front or rear wheels
       •  A lift truck steers more easily loaded than empty
       •  A lift truck is often driven as much in reverse as in forward gear

Employees who are assigned to operate powered  industrial trucks and/or lift trucks should be given a
certification card and should be required to carry it. "Rules of Operation" - Safety rules of operation will
be given to all industrial truck and lift truck operators.  These rules are described in Attachment 2.6 of
this chapter.
2.3 DOCUMENTATION

Documentation for all EPA required training courses and field certifications must be maintained by the
branch or office  safety representative, the GLNPO Safety Manager, the Branch or Division Safety
Representative, the EPA Safety Manager, and the EPA Training Officer.  In all cases, each attendant of
an EPA required safety course must complete a Standard Form 182 prior to training and forward it to the
Regional Training Officer. The information contained in this form will establish a permanent record and
be maintained,in the Human Resources Branch Training Data Management System.

The GLNPO Safety  Manager will record and certify that annually required training courses have  been
attended by each field inspector or field personnel and issue proper documentation. The GLNPO Safety
Manager will  also be responsible for maintaining annual medical monitoring and respirator fit testing
certification for appropriate field personnel.
2.4 MEDICAL MONITORING SELECTION POLICY

The Regional Medical Monitoring Selection Policy is detailed in Regional Order 3130 and is to be
followed by all EPA Region 5 employees to whom it applies.  The purpose of the Regional policy is to
ensure consistency in EPA's approach to mandatory participation in the Medical Monitoring Program and
to ensure that EPA provides  the most comprehensive health protection possible for its employees.  This
program is specifically designed to monitor the health of employees whose work regularly or periodically
poses the possibility of exposure to hazardous materials.

Medical monitoring may consist of a series of blood chemistry tests, urinalysis, pulmonary function tests,
hearing  testing, and chest x-rays.  Other types of monitoring may be necessary  based on the various
hazards  encountered by individual personnel. A physician is responsible for identifying and explaining
the significance of all findings.  No employee should leave the health unit with unanswered  questions.
Baseline or preplacement examinations are conducted prior to a job assignment where exposure to toxic
substances or hazardous materials may be possible.

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                                                          SECTION: CHAPTER 2
                                                          VERSION: FINAL/MAY 1997
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Three categories  in  which  Medical Monitoring is mandatory  are  Hazardous Waste Site Workers,
Laboratory Personnel, and Other Field Personnel or Inspectors who  are exposed to toxic chemicals.
Based on this policy, all GLNPO employees who work with potentially toxic chemicals must be monitored
for exposure.

The written Medical Monitoring policy is described in Appendix B of this manual.
2.5 ADDITIONAL HEALTH AND SAFETY COURSES

As well as courses that are required for field and laboratory personnel, other courses will be offered that
are intended for all employees, including Hazard Communication Training for office workers who do not
routinely work in unsafe situations.  Courses for non-field/lab personnel will be offered that  address
potentially serious health risks. Some courses will be offered consistently throughout the year while others
will be specially scheduled.
2.5.1  Occupational Noise Exposure

In 1983 OSHA promulgated a hearing conservation amendment to the 1971 noise standard. This standard      •
defines an effective program and requires that a hearing conservation program be implemented if workers
are exposed to levels equal to or greater than 85 dB measured on the A scale (slow response).  When
employees are subjected to noise at or above this PEL, regulations state, "the employer shall administer
an effective hearing conservation program". Note: EPA employees and contractors, including all GLNPO
personnel, are required to wear hearing protection in all work spaces where noise levels reach or exceed
85 dBA.

The hearing conservation program for GLNPO  research  facilities is described in Appendix C of this
manual.

2.5.2  Hazard Communication

GLNPO will ensure that a Hazard Communication Program is in place on all research vessels and that all
non-laboratory personnel aboard the vessels are familiar with the program.  The Hazard Communication
Program will  be based on the Occupational Safety and Health  Administration's regulation stated in 29
CFR 1910.1200.  The program will contain a chemical inventory list, material safety data sheets, proper
labeling requirements,  and employee  training specifications.  The Hazard Communication Program for
GLNPO is described in Appendix D of this manual.  Laboratory and Field personnel are required to
receive training in accordance with the OSHA regulations 29 CFR 1910.1450 "Occupational Exposures
to Hazardous Chemicals in Laboratories (refer to section 2.1.1 of this chapter  for training requirements).


Note:   All laboratory personnel aboard the vessels are protected under the OSHA health standard 29 CFR
        1910.1450 "Occupational Exposure to Hazardous  Chemicals in Laboratories".

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                                                          SECTION: CHAPTER 2
                                                          VERSION: FINAL/MAY 1997
                                                          PAGE: 8 of 9
2.5.3  Confined Space Entry

The Occupational Safety and Health Act of 1970 led to the development of the Safety Training and
Education Standard (29 CFR 1926.21) for confined spaces.  This standard defines a confined space and
requires that employees who enter confined spaces are trained to recognize the hazards of a confined space
and know the precautions to take when entering and working in  such a space.  Training is also required
for the use of appropriate personal  protective and emergency equipment when working in or  around
confined spaces.

After  review of fatality and injury data, the Occupational Safety and Health Administration found the
existing standards to be inadequate in protecting workers from the hazards of confined spaces. In June,
1989, OSHA proposed a new rule for the protection of workers in general industry who work in confined
space1;  This rule would require the development ^f a permitting system for confine J space entry, and
would address training, monitoring, and equipment necessary for  entry into confined spaces.  As of April
15, 1993, the Permit-Required Confined Spaces regulation has become effective as stated in 29 CFR Part
1910.146, Occupational Safety and Health Administration (OSHA), U.S. Department of Labor.  Further
information concerning the Confined Space Entry Program and its requirements is described in Appendix
E of this manual.

2.5.4  Hot Work Procedures

If proper safeguards are not implemented, activities such as cutting, welding, heating, grinding or other
activities that produce a flame or spark have the potential  for creating  a fire or explosion.  To minimize
hazards  associated with hot work,  Standard Operating Procedures (SOP) should be developed and
followed.

These procedures should include measures for protecting the worker, the establishment  of  a hot work
permitting system, monitoring for chemical exposure, and monitoring for fire and explosion potential.
When welding, cutting,  or heating,  all personnel and contractors must comply with OSHA 29  CFR
1926.353 for Ventilation and Protection in Welding, Cutting, and Heating During Construction Activities.
OSHA 29 CFR 1910.252, General Requirements, must also be followed. Additional information outlining
Hot Work Procedures is described in Appendix F of this  manual.

2.5.5  Lockout/Tagout

The purpose of lockout/tagout is to control the release of potentially hazardous energy. The  unexpected
energization of machines or equipment, or release of stored energy, can cause injury or death. Based on
this definition,  GLNPO's  Lockout/Tagout program is based on the  Occupational  Safety  and  Health
Administration's (OSHA) Control of Hazardous  Energy Sources Standard found in 29 CFR 1910.147.
The program is designed to safeguard the health and well being of GLNPO and contractor personnel from
a variety of energy sources when performing daily repairs, maintenance, and servicing of their operating
equipment.  To minimize hazards associated with these activities, personnel must be trained to  follow
proper lockout/tagout procedures specified in the written program.  The GLNPO Lockout/Tagout Program
includes  energy source  surveys,  designations of authorized personnel, affected  personnel,  training,
lockout/tagout methods and procedures, restoring equipment to use, and outside contractor information
exchange. The  GLNPO  Lockout/Tagout Program is described in Appendix G of this manual.

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                                                        SECTION: CHAPTER 2
                                                        VERSION: FINAL/MAY 1997
                                                        PAGE: 9 of 9
2.5.6  Bloodborne Pathogens

If it is reasonably expected that an employee may be exposed to human blood or other potentially
infectious materials, employers must protect their employees from the hazards of bloodborne pathogens
and comply with the OSHA Bloodborne Pathogen Standard, 29 CFR 1910.1030. The standard requires
procedures for the use of universal precautions, engineering controls, personal protec ive equipment, proper
housekeeping, training, and handling of regulated waste.  Due to the isolated nature of GLNPO research
vessel shipboard operations, emergency First Aid response may be necessary and exposure to potential
bloodborne pathogens may occur. Based on this assessment, a Bloodborne Pathogen Program has been
developed for GLNPO research vessels and is described in Appendix H of this manual.

2.5.7   Personal Protective Equipment (PPE)

Employees performing tasks requiring the use of personal protective equipment must receive training in
the use and limitations of the equipment. Training and program requirements can be found in OSHA 29
CFR 1910.132 and EPA Order 1440.2.

A written Personnel Protective Equipment Program  for GLNPO is  described in Chapter 5  and
Appendix I of this manual. All Emergency Response Personnel aboard the GLNPO Research Vessels
must meet the requirements specified in the program.

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                                         SECTION: CHAPTER 2, ATTACHMENT 2.1
                                         VERSION: FINAL/MAY 1997
                                         PAGE: 1 of 1
Attachment 2.1
                                   Training Date:
                                   Time:	
                                   Conducted By:
               24-HOUR LABORATORY SAFETY TRAINING AGENDA
                  GREAT LAKES NATIONAL PROGRAM OFFICE
                        RESEARCH VESSEL PERSONNEL
1.     IDENTIFICATION OF HAZARDOUS MATERIAL

2.     POTENTIAL SOURCES OF EXPOSURE

3.     TOXICOLOGY

4.     METHODS OF CONTROL

      A. Flow Relationships in Hazardous Materials Laboratories

      B. Ventilation

      C. Work Practices (Chemical Handling Protocols, Handling and Labeling)

5.     MONITORING

6.     PERSONAL PROTECTIVE EQUIPMENT

      A. Clothing

      B. Emergency Eyewash & Showers

      C. Respiratory Protection

7.     SPILL CONTROL

8.     WASTE AND STORAGE MANAGEMENT

9.     CHEMICAL HYGIENE  PLAN

10.    CONTINGENCY PLAN

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                                                    SECTION: CHAPTER 2,  ATTACHMENT 2.2
                                                    VERSION: FINAL/MAY 1997
                                                    PAGE: 1 of 3
Attachment 2.2 - GENERAL LABORATORY SAFETY RULES

All laboratories and  analytical procedures  have certain dangers in common.   Almost all analytical
procedures use chemicals that are considered toxic  or hazardous; examples  include acids, bases, and
organic solvents.  The samples for analysis often contain unknown biological  hazards or hazardous and
toxic chemicals. For these reasons, certain safety precautions are necessary throughout all laboratories and
whenever handling an unknown sample.

Each laboratory and analytical procedure will have safety rules specified in the Laboratory Safety Manual
or Standard Operating Procedure. These manuals and procedures will be specific to each laboratory,
however,  the following  laboratory safety rules  listed below will  apply  to  all U.S.  EPA Region  5
laboratories:

        1.      All exits and passageways must be unobstructed and allow  free exit.

        2.      Eating, drinking,  smoking or applying cosmetics in the laboratory is  prohibited.

        3.      Appropriate personal  protective  equipment must be worn for  each procedure.   This
               includes lab coats, gloves, safety glasses, goggles, and occasionally respirators. No bare
               legs, feet, sandals or perforated shoes are allowed.

        4.      Pipetting by mouth is prohibited.

        5.      All safety signs should be observed and obeyed.

        6.      All toxic waste must be properly disposed according to RCRA regulations.

        7.      Flammable, toxic or radioactive material must be stored in  approved  containers.

        8.      All containers of chemicals and samples shall be labeled clearly and correctly.

        9.      All gas cylinders must be firmly  secured to prevent falling.

        10.     All  electrical equipment  should be properly  grounded and  electrical cords inspected
               periodically to ensure that they are in good working condition.

        11.     Floors and surface working areas are to be kept clean, dry  and free  from corrosive
               chemicals.  Spills must be cleaned up immediately.

        12.     Initiation of a laboratory analysis  or experiment, not fully described in  Standard Methods,
               AQC  Manual or  other procedural source, must be preceded by a  survey  of existing
               literature.   The toxicity and other hazards  connected with the  reactants and products
               should be investigated thoroughly before starting any laboratory  reaction.

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                                                   SECTION: CHAPTER 2, ATTACHMENT 2.2
                                                   VERSION: FINAL/MAY 1997
                                                   PAGE:  2 of 3
CONTINGENCY PLANS

Contingency plans must be developed and implemented in cases of accidents or emergencies.  A spill
contingency plan is necessary when toxic or hazardous substances are on site. Personnel must be trained
in spill cleanup procedures. Depending on the size of the laboratory and the operations performed  in the
laboratory, a special team may be needed for emergencies.   At smaller installations, the local fire
department or emergency response team may be contacted  for emergencies. Emergency response  teams
will require training in respiratory protection, first aid, and  spill cleanup.  The duties of the team  must be
clearly defined.  Evacuation plans should  be distributed, posted and updated yearly.
VENTILATION REQUIREMENTS

Proper ventilation must be provided for analytical procedures and especially those involving the use of
toxic  substances.  Most laboratories will  be equipped with fume hoods to provide proper ventilation.
Fume hoods must be tested semi- annually to assure proper flow rates and containment.  Maintenance
must be conducted on a regular basis to insure proper functioning. Exhaust air from glove boxes must
be  treated  by  filtration, reaction, absorption,  adsorption,  electrostatic  precipitation or  incineration
depending on the chemical compounds being used.  All exhaust air from primary containment equipment
must be discharged by roof- mounted blowers  and dispersed clear of occupied buildings and air intakes.

Additionally, a mechanical exhaust ventilation system must be in place to control laboratory room air
movement. The air supply must originate from areas where the potential for contamination is low, and
exhaust into outside areas.
STORAGE REQUIREMENTS

Proper storage techniques must be followed and are detailed in the following sections.


STORAGE OF COMPRESSED GAS CYLINDERS

Only compressed gas cylinders required for immediate use are allowed to be stored in laboratory work
areas.  Separate areas should be provided for storage of all other gas cylinders.  Empty and full cylinders
should not be stored together.  Oxygen cylinders  must not  be  stored near flammable gas cylinders.
Cylinders of compressed gases must be stored with  the valve end up and securely fastened to a wall or
immovable object to prevent the cylinder from falling. All cylinders must be securely fastened (strapped
or chained) to prevent falls.  All cylinders must be kept away from heat sources.

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                                                   SECTION:  CHAPTER 2,  ATTACHMENT 2.2
                                                   VERSION:  FINAL/MAY  1997
                                                   PAGE:  3 of 3
STORAGE OF CHEMICALS

All chemicals or waste materials  in the laboratory, stockroom, or bulk storage must be stored according
to their chemical compatibility.  Storage control will minimize the potential for accidental mixing by
spillage, breakage,  or fire.   Major  storage areas  are  required for the following types of chemical
compounds: flammable solvents, corrosive liquids, and strong oxidizers. Additional storage may include
areas for radioactive materials, biological hazards, highly toxic materials and compressed gases.

When working in laboratory areas, only the minimum amount of chemical needed for analysis operations
should be stored.  Separate storage areas (stockrooms) should be provided for the main bulk of chemicals.
Incompatible chemicals should either be stored in separate safety storage cabinets, divided by a wall, or
enclosed by a tray to  prevent accidental mixture. These storage areas and containers should always be
properly labeled.

The main storage areas should be divided to accommodate incompatible chemicals.  Depending on the
amount of chemicals in storage, approved safety storage cabinets can be used. If necessary, storage rooms
can also be used.

No more than five one-liter containers of Class I and II materials  should be stored in open laboratory
areas.   Open laboratory areas include reagent shelves  and  bench  tops.  All glass containers  must be
protected by an outer  sheath or made of  unbreakable glass.

Storage areas for waste collection should be provided and properly designated.  Provisions for containment
and separation of incompatible waste materials are also  required.

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                                            SECTION:  CHAPTER 2,  ATTACHMENT 2.3
                                            VERSION: FINAL/MAY 1997
                                            PAGE:  1 of 1
Attachment 2.3
                                     Training Date:.
                                     Time:	
                                     Conducted By:_
                4-HOUR LABORATORY SAFETY TRAINING AGENDA
                   GREAT LAKES NATIONAL PROGRAM OFFICE
                         RESEARCH VESSEL PERSONNEL
1.     CHEMICAL HEALTH AND SAFETY RESPONSIBILITIES

      A.     Federal Safety and Health Regulations

      B.     EPA Occupational Health and Safety Program

      C.     OSHA 1910.1450 Occupational Exposures to Hazardous Chemicals in
             the Laboratories.

2.     RIGHT TO KNOW

      A.     Chemical Inventory

      B.     Hazard Identification

      C.     MSDS

      D.     Labeling

      E.     Training

3.     CONTROLS

      A.     Chemical Handling and Storage

             1.     Flammable Materials
             2.     Corrosives
             3.     Highly Toxic Chemicals

      B.     Ventilation

      C.     Personal Protective Equipment

4.     SOPs/SAFETY PLANS/EMERGENCY PROCEDURES

5.     SPILL CONTROL/HAZARDOUS WASTE

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                                              SECTION: CHAPTER 2, ATTACHMENT 2.4
                                              VERSION: FINAL/MAY 1997
                                              PAGE:  1 of 2
Attachment 2.4
                                       Training Date:.
                                       Time:	
                                       Conducted By:
                                 TRAINING AGENDA
                                         FOR
                    GREAT LAKES NATIONAL PROGRAM OFFICE
                           RESEARCH VESSEL PERSONNEL
I.       SUBJECT

             1.   GLNPO Chemical Hygiene Plan

                    general overview
                 -   use of PPE
                    health and safety SOPs for analytical procedures
                    MSDS overview

             2.   Environmental and Health and Safety Procedures

                    hazmat/waste transportation
                    hazmat storage/spill prevention
                    hazardous/common waste
                    contingency  plan (plan,  SCBA, APR,  spill cart, instruments, fire,  identify
                    team  members)

             3.   General

                    MSDS review for specific materials
                    safety committee
                    lockout/tagout awareness
                    general comments

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                                           SECTION:  CHAPTER 2, ATTACHMENT 2.4
                                           VERSION:  FINAL/MAY 1997
                                           PAGE:  2 of 2

II.     PARTICIPANTS

Name - Print                        Name - Sign                     Date

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                                                   SECTION:  CHAPTER 2,  ATTACHMENT 2.5
                                                   VERSION:  FINAL/MAY 1997
                                                   PAGE:  1 of 2
Attachment 2.5  POWERED INDUSTRIAL TRUCKS (FORK LIFTS)


                                  RULES OF OPERATION

Safe rules of operation must be followed by all powered industrial truck drivers. The drivers should keep
the following rules conveniently located, refer to them frequently, and use them as a checklist for safety.
These rules have been developed in accordance with ANSI-B56.1-1993 (Revised).

   1.   At the beginning of each use, check brakes, steering controls, forks, hoists, warning devices and
       lights.  Report any  defects  to the supervisor immediately.   Also check to  see  that the fire
       extinguisher is in place and properly serviced.

   2.   Always face the direction of travel.

   3.   Before you start driving, look to see that no person or object is in your path.  Always look before
       backing up.

   4.   Do not drive over objects lying on the floor such as trash, lumber and pipes.  These could damage
       the truck, shift or topple the load, or flip out and strike another employee.

   5.   Use low gear when going down ramps or steep grades. On upgrades, keep the load in front. On
       downgrades, keep the load in back.

   6.   Under normal traffic conditions, keep to the right.

   7.   Avoid quick starts or turns, and jerky  stops; always come to a complete stop before reversing the
       direction of travel.

   8.   Keep a safe distance between vehicles at all times (three truck lengths).

   9.   Make a complete  stop at  all doors, corners, exits and stop signs.

   10.  No horseplay at any time!

   11.  Keep alert—concentrate on the job at hand.

   12.  Remember that pedestrians have the right of way at all times.

   13.  Never drive with  wet or greasy hands.  If necessary, keep a towel or rag handy at all times.

   14.  Reduce speed on wet and slippery floors.

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                                                 SECTION: CHAPTER 2,  ATTACHMENT 2.5
                                                 VERSION: FINAL/MAY 1997
                                                 PAGE:  2 of 2
15.  Whether loaded or empty, carry forks and platforms on lift trucks as low as possible.  This lowers
     the center of gravity and reduces the possibility of overturning the truck or dumping the load.

16.  Don't run trucks onto elevators unless authorized to do so. Be sure you have sufficient clearance
     before entering any elevator or going through a doorway.  Once the truck is in the elevator, shut
     off the power, set the brakes and stay in the operator's seat in case of an emergency.

17.  Check the clearance of any overhead crane or other object before raising forks or tiering.

18.  Never use your truck as an elevator for other employees (for example, to service light fixtures or
     stock material).

19.  When leaving the truck at any time, put the forks or platforms  in the  down position, shut off the
     power, set the brakes and remove the ignition key.

20.  Never leave a truck  in an aisle.  Park the truck to one side, so traffic will not be blocked.

21.  Before driving the truck into a freight car or trailer, always secure the bridging plate with bolts
     or pins so that  it cannot move when  the power wheels of the truck pass over it; and before
     moving the truck onto the bridge or into the carrier, walk into the freight car, truck or trailer and
     check the floor  for holes or weak spots (the weight of your truck might break the floor).

22.  Keep your feet and legs inside the guard, which is there to protect you.

23.  Drive with  extreme care, especially during shift changes.

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                                                                 SECTION: CHAPTER 3
                                                                 VERSION: FINAL/MAY 1997
                                                                 PAGE:  1 of 3
Chapter 3 - COMPLIANCE REVIEW AND STANDARD OPERATING PROCEDURES
3.1 OVERALL POLICY

All construction, safety-related procurements, and safety-related contract activity must be initially reviewed
by the Regional Safety Manager or other professionals working under the direction of the Regional Safety
Manager.  The initial review of activities is designed to assess any issues which may impact the safety,
occupational health or  environmental compliance of GLNPO employees, contractors,  and  the general
public. Specifically, activities for initial review by the Regional Safety Manager include hazardous waste
handling,  transportation,  and disposal.   Supervisors,  managers,  property  control  personnel, and
procurement personnel have a responsibility to assist the Regional Safety Manager in the maintenance and
enforcement of Health and Safety  Office review policies.

As such, visiting scientists (non-EPA or EPA contractors) must have health and safety standard operating
procedures, spill programs, and training required to implement spill cleanup procedures  if necessary.
3.2    SAFETY & HEALTH REVIEW OF NEW CONSTRUCTION/REPAIRS AND
       ALTERATIONS

All new facility construction activity and significant repairs or alterations to GLNPO facilities must be
reviewed by the Regional Safety Manager or other professionals working under his/her direction.  The
review will be initiated at the planning and design phases and continue throughout construction.  The
Safety Manager will recommend design changes or design applications relative to safety, occupational
health, fire prevention and protection, hazardous waste handling and disposal, and related program areas.
As necessary, the  Safety Manager may enlist and coordinate the services of specialized experts in such
fields as fire protection, engineering, ventilation, hazardous waste, and other related fields. All facility
activity impacting life and safety must have the approval of the Regional Safety Manager before it  can
be initiated.

3.3    SAFETY & HEALTH REVIEWS OF MARINE REPAIRS AND ALTERATIONS

All construction, repairs or alterations  to GLNPO vessels, labs or other marine equipment in any way
impacting safety, sanitation, occupational health, fire prevention and protection, environmental compliance
or related  issues must  have the approval of the Regional Safety Manager before such activity is begun.
The ship operations contractor should immediately report any potential hazards to the project officer,  the
GLNPO Safety Manager, and the Regional Safety Manager.

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                                                               SECTION: CHAPTER 3
                                                               VERSION:  FINAL/MAY 1997
                                                               PAGE: 2 of 3
3.4    REVIEW OF  HAZARDOUS  MATERIALS  AND HAZARDOUS  WASTE  DISPOSAL
       PROCUREMENT

Procurement requests for hazardous materials and hazardous waste disposal at  GLNPO facilities must be
approved and authorized in written form by the Safety Manager or an individual who has been designated
by the Safety Manager.  This procedure  is necessary due to the complexity of hazardous materials and
hazardous waste disposal regulations, as well as potential agency liability. Standard Operating Procedures
for Hazardous Materials and Hazardous Waste Disposal may be found as an Attachment to the Chemical
Hygiene Plan found  in Appendix J of this manual.
3.5    HEALTH AND SAFETY REVIEW OF CONTRACTS

Any contract which involves field or laboratory work or otherwise appears to impact health and safety
must be reviewed and approved by the Regional Safety Manager while in the  initial planning stage.  All
EPA contracts must contain provisions which require the contract employee to follow all safely, health,
and environmental compliance rules and regulations. If necessary, applicability of compliance rules and
regulations will be determined by the Regional Health and Safety Manager.


3.6    REVIEW OF MISCELLANEOUS PROCUREMENT IMP ACTING SAFETY, HEALTH OR      ^
       ENVIRONMENTAL COMPLIANCE

Any procurement issue which has not been addressed in this manual, but appears to impact safety, health,
hazardous material, or hazardous waste handling and disposal, should be brought to the attention of the
Regional Safety Manager. The Regional Safety Manager will then determine what action, if any, needs
to be taken.
3.7    STANDARD OPERATING PROCEDURE POLICY

Written Health and Safety Standard Operating Procedures (SOPs) must be developed for specific tasks
(eg. pesticide inspections, PCB inspections, RCRA permit inspection, RCRA enforcement of TSDs, etc.)
related to field  or  laboratory  operations.  Within  these SOPs, all  safety,  occupational health and
environmental compliance (hazardous waste transport and disposal) issues should be addressed.  Division
or Office management is responsible for the development of safety SOPs. The SOPs should be reviewed
by the appropriate Division or Office Safety Representative.  All SOPs should be readily available for
examination by the Regional Safety Manager during regularly scheduled inspections or surveys.

Health and Safety SOPs may address issues such as  Safety Procedures, personal protective equipment,
training requirements (formal and on-the-job), medical monitoring and respirator fit testing requirements.
Special requirements, such as specific types of respirators and confined space entry procedures, may also
be addressed.  If appropriate,  Regional SOPs may be used to  supplement laboratory or field SOPs.

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3.8    FIELD SOPs

Standard operating procedures for GLNPO field operations, primarily sediment sampling, are described
in Chapter 10 of this manual.
3.9    LABORATORY CHEMICAL HYGIENE SOPs

Laboratory Chemical Hygiene SOP's on board GLNPO research vessels are described in Appendix L of
this manual.
3.10   MARINE SOPs

All marine sampling, recovery, and operational activities are required to have SOPs for specific activities
which impact safety, health, or environmental compliance.
3.11    EVACUATION PLAN

All GLNPO personnel must participate in facility evacuation training exercises and drills. They are also
expected to be familiar with operational  procedures identified in each Occupant Emergency Evacuation
Plan  for their place of work.  GLNPO personnel  are expected to  cooperate fully with the instructions
provided.  An Occupant Evacuation Plan is described in Appendix M of this manual.

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Chapter 3 - COMPLIANCE REVIEW AND STANDARD OPERATING PROCEDURES
3.1 OVERALL POLICY

All construction, safety-related procurements, and safety-related contract activity must be initially reviewed
by the Regional Safety Manager or other professionals working under the direction of the Regional Safety
Manager.  The initial review of activities is designed to assess any issues which may impact the safety,
occupational health or  environmental compliance of GLNPO employees, contractors, and the general
public.  Specifically, activities for initial review by the Regional Safety Manager include hazardous waste
handling,  transportation,  and disposal.   Supervisors,  managers,  property  control personnel,  and
procurement personnel have a responsibility to assist the Regional Safety Manager in the maintenance and
enforcement of Health and Safety Office review policies.

As such, visiting scientists (non-EPA or EPA contractors) must have health and safety  standard operating
procedures, spill programs, and training required to implement spill cleanup procedures if necessary.
3.2    SAFETY & HEALTH REVIEW OF NEW CONSTRUCTION/REPAIRS AND
       ALTERATIONS

All new facility construction activity and  significant repairs or alterations to GLNPO facilities must be
reviewed by the Regional Safety Manager or other professionals working under his/her direction.  The
review will be  initiated at the  planning and design  phases and continue throughout construction.  The
Safety Manager will recommend design changes or design applications relative to safety, occupational
health, fire prevention and protection, hazardous waste handling and disposal, and related program areas.
As necessary, the Safety Manager may enlist and coordinate the services of specialized experts in  such
fields as fire protection, engineering, ventilation, hazardous waste, and other related fields.  All facility
activity impacting life and safety must have the approval of the Regional Safety Manager before it can
be initiated.

3.3    SAFETY & HEALTH REVIEWS OF MARINE REPAIRS AND ALTERATIONS

All construction, repairs or alterations to  GLNPO vessels, labs or other marine equipment in any way
impacting safety, sanitation, occupational health, fire prevention and protection, environmental compliance
or related issues must have the approval of the Regional Safety  Manager before such activity is begun.
The ship operations contractor should immediately report any potential hazards to the project officer, the
GLNPO Safety Manager, and the Regional Safety Manager.

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3.4    REVIEW OF  HAZARDOUS  MATERIALS  AND HAZARDOUS  WASTE  DISPOSAL
       PROCUREMENT

Procurement requests for hazardous materials and hazardous waste disposal at  GLNPO facilities must be
approved and authorized in written form by the Safety Manager or an individual who has been designated
by the Safety Manager.  This procedure  is necessary due to the complexity of hazardous materials and
hazardous waste disposal regulations, as well as potential agency liability. Standard Operating Procedures
for Hazardous Materials and Hazardous Waste Disposal may be found as an Attachment to the Chemical
Hygiene Plan found  in Appendix J of this manual.
3.5    HEALTH AND SAFETY REVIEW OF CONTRACTS

Any contract which involves field or laboratory work or otherwise appears to impact health and safety
must be reviewed and approved by the Regional Safety Manager while in the initial planning stage.  All
EPA contracts  must contain provisions which require the contract employee to follow all safety, health,
and environmental compliance rules and regulations. If necessary, applicability of compliance rules and
regulations will be determined by the Regional Health and Safety Manager.
3.6    RE VIEW OF MISCELLANEOUS PROCUREMENT IMPACTING SAFETY. HE ALTHOR
       ENVIRONMENTAL COMPLIANCE

Any procurement issue which has not been addressed in this manual, but appears to impact safety, health,
hazardous material, or hazardous waste handling and disposal, should be brought to the attention of the
Regional Safety Manager. The Regional Safety Manager will then determine what action, if any, needs
to be taken.
3.7    STANDARD OPERATING PROCEDURE POLICY

Written Health and Safety Standard Operating Procedures (SOPs) must be developed for specific tasks
(eg. pesticide inspections, PCB inspections, RCRA permit inspection, RCRA enforcement of TSDs, etc.)
related to field  or  laboratory  operations.  Within  these SOPs, all  safety,  occupational health and
environmental compliance (hazardous waste transport and disposal) issues should be addressed.  Division
or Office management is responsible for the development of safety SOPs. The SOPs should be reviewed
by the appropriate Division or Office Safety Representative.  All SOPs should be  readily available for
examination by the Regional Safety Manager during regularly scheduled inspections or surveys.

Health and Safety SOPs may address issues such as Safety Procedures, personal protective equipment,
training requirements (formal and on-the-job), medical monitoring and respirator fit  testing requirements.
Special requirements, such as specific types of respirators and confined space entry procedures, may also
be addressed.  If appropriate,  Regional SOPs may be used to  supplement laboratory or  field SOPs.

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3.8    FIELD SOPs

Standard operating procedures for GLNPO field operations, primarily sediment sampling, are described
in Chapter 10 of this manual.
3.9    LABORATORY CHEMICAL HYGIENE SOPs

Laboratory Chemical Hygiene SOP's on board GLNPO research vessels are described in Appendix L of
this manual.
3.10   MARINE SOPs

All marine sampling, recovery, and operational activities are required to have SOPs for specific activities
which impact safety, health, or environmental compliance.
3.11    EVACUATION PLAN

All GLNPO personnel must participate in facility evacuation training exercises and drills. They are also
expected to be familiar with operational  procedures identified in each Occupant Emergency Evacuation
Plan  for their place of work.  GLNPO personnel are expected to cooperate fully with the instructions
provided.  An Occupant Evacuation Plan is described in Appendix M of this manual.

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Chapter 4 - RESEARCH VESSEL HEALTH AND SAFETY
GLNPO RESEARCH VESSEL HEALTH AND SAFETY - INTRODUCTION

Unique to GLNPO research vessels are their on-board laboratories.  This feature, in addition to normal
marine operations, requires adherence to rigorous  health and safety procedures.  Each  vessel must
develop and implement a Chemical Hygiene Plan designed to protect laboratory personnel from exposure
to health  hazards during work operations. Also, all GLNPO shipboard personnel must be familiar with
marine safety practices associated with large and small boat operations.  Although each vessel is staffed
with an experienced professional crew, it is the policy of GLNPO to ensure that all personnel aboard
each research vessel receive health and safety training  relative to the  specific  hazards they may
encounter while on Loard.
4.1 GLNFO CHEMICAL HYGIENE PLAN

Since laboratory work operations typically involve exposure to or use of toxic substances, specific health
and safety programs are required. These programs are not merely limited to laboratory safety training.
The Occupational Safety and Health Administration (OSHA) requires facilities engaged in the laboratory
use of hazardous chemicals to develop and implement a "Chemical Hygiene Plan" as specified in 29
CFR 1910.1450.  A Chemical Hygiene Plan contains procedures and work practices designed to protect
laboratory personnel from exposure to health hazards during their work operations. As such, a Chemical
Hygiene Plan must be developed for each GLNPO laboratory. The Chemical Hygiene Plan for GLNPO
research vessels is attached as Appendix J of this manual.
4.2 SAFETY ORIENTATION VIDEO

New employees on board any GLNPO research vessel are required to receive site- specific safety
training.  Such training will include basic safety regulations, evacuation procedures, location of PPE,
and specific hazards to which they may be exposed while on board the vessel. As part of this training,
an instructional video will be presented and personnel will be required to document that they understand
its contents. Additionally, all GLNPO personnel and contractors will be required to know the location
of the Health & Safety Manual and the Material Safety Data Sheets.  A GLNPO vessel orientation
narrative appears as Attachment  4.1 of this chapter.
4.3 BOAT HANDLING AND SEAMANSHIP

As described  in Chapter 1 of this manual, the Great Lakes National Program Office maintains a fleet
of research vessels specifically outfitted for limnological research.  These vessels range in size from 33
feet to 180 feet with sampling missions lasting from 1 to 30 or more days. Those vessels outfitted for
daily sampling missions are capable of maneuvering in the rivers, creeks and streams which empty into
larger bodies of water. The large vessels, on the other hand, are equipped for lengthy sampling missions
in the vast expanses of the Great Lakes.

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While many health  and  safety practice  procedures apply to both small and large vessels, there are
specific  differences.  On board a small boat, crew movement, docking, and anchoring are significant
safety issues. However,  on large vessels, use of heavy equipment becomes a primary safety concern.
To achieve maximum health and safety aboard all GLNPO vessels, it is the ultimate  responsibility of
each crew member to comport themselves in a  safe and competent manner.

4.3.1  SAFE BOATING OPERATIONS - SMALL AND LARGE VESSELS

Safe boating is based on good sense, education  and experience.  Each GLNPO research vessel will be
piloted by an accomplished boat operator and a chief scientist will monitor all activities.  It is the
responsibility of each crew member to report any unsafe condition and to work only within their ability.
Should crew members encounter situations about which they are uncertain, they must report to the
appropriate ship personnel before taking action. There is always an element of danger when working
on the water.

4.3.2  PERSONAL FLOTATION DEVICES

Life jackets (personal flotation devices) must be worn any time  the vessel is away from the dock and
you are working on deck. A range of sizes are  available. Eighty-five percent of the people who have
died in boating accidents were not wearing PFDs.                                                     ^

Three types of PFDs frequently in use on EPA research vessels. Type 1  offers the greatest buoyancy
and will automatically turn an unconscious person face up while in the water. However, these PFDs
are  bulky and difficult to work in.  The second  type of PFD, also called a keyhole vest or type 2 PFD,
will keep an unconscious person face up but not as efficiently as a Type 1 PFD. The Type 2 life jacket
is used more often when the boat is working close to shore where a quick, land-based rescue  is possible.
The third type of PFD is a flotation aid or work vest (Type 3 PFD).  The Type 3 PFD permits the most
freedom of movement but is not designed to hold an unconscious person upright in the water.

In addition to the above listed PFDs, there are two other types of PFDs which may be worn. The first
type is what is commonly referred to as a Mustang Suit.  Mustang Suits are classified as a type 5 PFD.
Most Mustang suits will not right an unconscious person in the water.  A Mustang Suit are made to help
maintain body heat and may be worn during periods of cold weather conditions. The  second type is a
survival suit. A survival suit would be worn in the event of an abandon ship. During  routine drills all
persons  are required to donned the suit and are instructed in proper water entry.

4.3.3  MAN OVERBOARD PROCEDURES

If witness to someone falling overboard, the following procedures are to be executed:

        1.  Scream "Man Overboard" to get the attention of others but do not take your eyes off the
           person in the water.  If possible,  throw anything that floats overboard to help mark the
           area in which the person went overboard. If the person is within range, throw  them a life
           ring.

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       2.  Point to the position of the person in the water.
        3.  At the first cry of "Man Overboard", the boat operator will freeze the Loran C display and
           place the engines in neutral until the location of the person is known.  Once the location is
           known a rescue pattern can be executed.

        4.  Prepare to throw the life ring when the victim  comes within range.

        5.  After the victim  is pulled to the side of the vessel, the crew can assist the person to get back
           on board at the direction of the boat operator.  No one is permitted to go  into the water
           to retrieve the victim unless directed by the boat operator. Jumping into the water in
           order to "save" someone often results  in two victims requiring rescue.

        6.  If the victim is not recovered immediately or is lost from sight, the Coast Guard should be
           notified immediately as well as other boats  in the area that can assist in a search effort.

        7.  The boat operator will  begin a  search pattern using the  position placed in the Loran C
           memory.

4.3.4 FIRE

The  possibility of fire on board ship is always of great concern.  If fire should occur,  it  may be fought
without the assistance of fire fighting professionals.  Consequently, it  is important that  research vessel
personnel keep in mind fire  safety when considering all shipboard activities.

When first aboard, vessel personnel will be apprised of the location of fire extinguishers  and review the
ship's fire fighting plans and procedures.  Decisions regarding fire fighting assignments will be made
at the beginning of each survey and the boat operator will be responsible for the plan and  the assignment
of fire  fighting stations and duties.

4.3.5 WEATHER

Weather conditions can change rapidly on the water.  Therefore, it is important to always be aware of
these changes and to act accordingly.

4.3.6 DISTRESS SIGNALS

Despite being as well prepared as  possible, there may be times when assistance is needed.  In addition,
the law requires that assistance be rendered to other  boaters in distress.

Visual  hand signals may be used to  attract attention and indicate a need for help.  Additionally, flares
and dye markers may be used to signal for assistance. Channel  16 on the marine radio is  the channel
reserved for emergency transmissions. If a "MAYDAY" distress call is heard from another vessel, do
not use Channel 16 and allow the Coast Guard to respond.  If a GLNPO research vessel  is in a position
to render assistance, contact the Coast Guard as soon as there is a break in radio traffic.

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4.3.7  FUELING
The greatest potential for fire occurs during the fueling of a vessel. Each year lives and boats are lost
due to explosions and fires related to fueling operations.

Standard Operating Procedures have been developed for various GLNPO vessels and can be found as
Attachment 4.3 of this chapter.

4.3.8  RULES OF THE ROAD

The operator of a vessel must have a thorough understanding of the nautical rules of the road.  The
following rules should be followed by all boat operators:

        1.  If you encounter a vessel coming head on, make a clear turn to the right to indicate your
           intention to pass the other vessel on your left.

        2.  You are to give way if a vessel is approaching your line of direction from your right.

        3.  Always keep in mind that the people aboard the other vessel may not know what they are
           doing so be ready to take evasive action if necessary.

        4.  Sailboats under sail always have the right of way.

        5.  If you are passing a vessel, you must stay clear.

        6.  You are responsible for  your wake and any damage that it may cause.  You must obey all
           speed limits and No Wake Zones.

4.4 SMALL BOAT OPERATIONS

In addition  to  use of personal flotation  devices,  man  overboard  procedures, fire safety, weather
awareness,  distress signals, fueling  procedures, and rules of the road, small boat  operations include
embarking, anchoring, and docking  activities.

EMBARKING PROCEDURES

The following procedures are used when leaving the dock:

        1.  Don personal flotation devices (PFDs).

        2.  Start engines and allow them to warm up.


        3.  Follow the boat operator's directions for releasing dock lines making sure that no lines are
           in the water.

        4.  NEVER CAST OFF FROM THE DOCK UNTIL ORDERED TO DO SO BY THE
           BOAT OPERATOR

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DOCKING PROCEDURES
When docking the boat, the boat operator needs to concentrate on the docking procedure. Try to avoid
conversation at this time, pay close attention to what is going on and be prepared to assist as directed
by the boat operator.  The following should be considered when docking:

       1.  The boat operator will assign duties.  Learn the docking procedures and be prepared to
           implement them.

       2.  Secure the fenders to the side of the vessel as directed by the boat operator.

       3.  Prepare bow and stern dock lines.  A crew member should standby each  line.

       4.  As the vessel conies into the dock, watch for any obstructions in the water that the  boat
           operator may not see and immediately  bring them to his/her attention.

       5.  Never jump from the vessel to the dock. One could fall and be crushed between the dock
           and the vessel.

       6.  Never use arms or legs to slow the boat as it comes into dock. The boat operator will use
           the boat's engines to do this.

       7.  As the boat enters the dock, make sure  that you are not blocking the boat operator's view.

ANCHORING PROCEDURES

An anchor is a device used to hold a vessel in  position while away from the dock.  One, two or three
anchors may be used to hold a vessel in position.  The following is the general procedure for deploying
and retrieving  anchors:

Deployment

       1.  Make sure the anchor line is attached to the anchor and the  boat.

       2.  Do not toss the anchor out away from the boat. Rather, lower it when signaled by the  boat
           operator.

       3.  As the boat reverses, take a turn around the cleat and let the  line out. Never allow the line
           to  run freely through your hand.  An attempt to stop a free-running line can deliver a burn
           or even a deep cut to your hand.

       4.  When you are letting line out, make sure you are not standing on the anchor line and that
           it is not tangled around your foot or any  other object in the boat.

       5.  The length of rope needed to be played out, called the  scope, is generally 6 to 8 times the
           depth of the water in which you are  anchoring.

       6.  When securing the anchor  line to the cleat, take at least two turns before cleating-off the
           anchor line.

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RETRIEVAL
        1.  As the boat moves forward and the strain is removed from the anchor line, release the
           anchor line and begin taking it in.  Always keep a turn on the cleat.

        2.  When the anchor line is running straight up and down, Iry  to pull up the anchor.  If the
           anchor is not free, turn the cleat a few times and signal the boat operator to run forward
           slowly while you keep the anchor line fast.  When the boat operator stops the forward
           movement of the boat, try once more to lift the anchor.  Replace the anchor on deck and
           secure it prior to getting underway.

KNOTS

There are a few basic knots that are useful when working on a vessel.  They include the cleat hitch,
figure eight knot, round turn and two half hitches, the bowline knot and the clove hitch.
4.5 LARGE BOAT OPERATIONS

Again, the use of personal flotation devices, man overboard procedures, fire safety, weather awareness,
distress signals, fueling procedures, and rules of the road, apply to large vessels, as well as small vessels.
The large vessels, however, are outfitted with heavy equipment such as "A"  frames, knuckle/pivot
cranes, rigging, and hoists.  Standard Operating Procedures for deck safety practices during use of this
type of heavy equipment must be addressed and enforced by the ship's operating contractor.

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                                                   SECTION: CHAPTER 4, ATTACHMENT 4.1
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Attachment - 4.1      WELCOME  ABOARD  STATEMENT  FOR  THE  R/V  LAKE
                      GUARDIAN

The United States Environmental Protection Agency, the ship's operations contractor, and the Master,
officers and crew of the R/V Lake Guardian welcome you aboard.  We hope your stay on board will
be a pleasant one.   Our goal will be to assist you to bring your research programs to a safe and
successful conclusion.

We appreciate your cooperation in accomplishing this goal and we ask that you review the material
in this handout.  Should you have any questions or encounter problems regarding the items presented
herein, please bring them to the attention of the ship's Master via the Chief Scientist.

This informational handout will provide some essential facts and useful information to insure you have
a safe and comfortable voyage.  Please do not hesitate to ask questions concerning any subject about
the ship. We will be happy to provide explanations and/or demonstrations when possible. If you find
you are having a problem, please bring it to the attention of the ship's Master (Captain), the Mate on
watch or the Chief Scientist.
OPERATING AUTHORITY

The Lake Guardian is operated for and under the control of the U. S. EPA's Great Lakes National
Program Office.  An operating crew of professional seamen is furnished by the ship's contractor.
Laboratory support is provided by personnel from EPA's on-site Central Regional Laboratory.  Lab
and ship safety overview is provided by an outside safety consulting firm.  When the ship is at sea,
a senior scientist from  U. S. EPA/GLNPO is assigned responsibility for all movements and activities
aboard the ship, in conjunction with the ship's Master.  These two individuals have the final word on
any issue which might arise while at sea.
MISSION

Lake Guardian's mission is to gather information about the chemical and biological conditions of the
Lakes, and to monitor the pollutant concentrations in water, sediments, air, fish, and other biological
matter.  On occasion, the ship may be used for other scientific interests, including research activities
of other federal, state and local agencies as well as academia.

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GENERAL DESCRIPTION

Lake Guardian is U. S. EPA's newest, largest and cleanest surveillance and monitoring vessel. It was
converted in 1990 from a former offshore oil field service vessel originally built in 1981. During the
conversion period, all of the structures aft of the exhaust stacks were added.

In 1991, the four  container labs and the HVAC (heating, ventilation and air conditioning) van  were
added to the ship, as well as the large stern U-Frame.  The ship is 180 feet in overall length, with a
beam (width) of 40 feet.  Depending on loading conditions, it can have a draft (underwater portion
of the hull) of up  to  11 feet. Lake Guardian is powered by two 1125  HP Caterpillar diesel engines,
each driving its own propeller shaft.  Electrical power is provided by three 135 KW Caterpillar diesel
engines.  A bow thruster, powered by a 300  HP Caterpillar diesel engine, aids in the ship's ability to
maneuver in close quarters. It is also used in station keeping.  When operating at full power, the ship
can make 13 miles per hour and will consume in excess of 100 gallons of fuel per hour.

LIFE ABOARD LAKE GUARDIAN

Knowledge of the ship's layout will assist you in accomplishing your  daily activities, and is vital in
determining the appropriate response for emergency situations, both real and simulated.  Please study
the deck plans, note the locations of fixed fire stations, portable fire extinguishers and abandon ship
stations.

Because of Lake Guardian's unique  mission, schedules and routines are  inherently  rigid.   It is
suggested that you keep abreast of vessel movements, scientific activities and the weather so that you
are aware of emerging conditions  which may affect you and/or your work aboard the ship.

Lake Guardian has berthing for up to 43 persons, including the crew.  Most staterooms set aside for
scientific personnel have two fixed bunks (upper and lower) as well as  a fold-down pullman bunk for
a third person.  These pullman bunks  are used only when a maximum complement of personnel is
carried aboard; some crews may have  to share accommodations.   Stateroom assignments are made
jointly by the ship's  Master and the Chief Scientist.

Each stateroom and bunk contains a card which designates the emergency mustering location for the
persons in that stateroom.

REPORTING ABOARD

Plan to report aboard at  least one hour before sailing time.  Upon arrival  you should report to the
Chief Mate, who will sign you aboard and provide an orientation of the  vessel's physical arrangements
including assigned berthing area.  Berthing assignments for science personnel are made by the Chief
Scientist.

Linens, towels, etc.  will be provided  when you arrive on board.  Upon departure from  the vessel,
kindly strip your bunk and store  the soiled linen  in the laundry  basket provided near the washing
machine on the port side of the main  deck,  forward.  Fresh linen will be issued once  per week, the
time of which will be announced  in advance.

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For your safety, you are  required to accomplish the following  immediately  after receiving your
berthing assignment:

        •  Insure that your life jacket is in place on your bunk.

        •  Insure that your survival suit is in place on your bunk.

Refer to the station bill posted in the mess deck and find your assigned life raft, fire and abandon ship
stations.

Become familiar with the signals for fire and abandon ship; a drill will be conducted upon departure
from the first port and at least once per week thereafter.

Safety Rule:
               One hand for you and one hand for the ship. Always keep one hand free. Make
               two  trips if necessary or get someone  to  help you.   In addition, all persons
               working or observing on deck must wear a work vest.  NO EXCEPTIONS.


PERSONAL POSSESSIONS

The ship's operating contractors cannot be responsible for the loss of personal possessions.  Items of
significant value should be left ashore, or given to the ship's Master  for  storage in the ship's safe.

SAILING TIME

Science personnel should plan to arrive  on board at least one  hour before sailing time.   Allow
sufficient time prior to departure  to turn in rental cars, load equipment, etc..  Sailing time may be
delayed at the Master's discretion if science/technical personnel report aboard in a condition which
he considers unsafe.  Further delays in sailing are announced should equipment malfunction occur,
or when late deliveries of equipment and supplies are experienced.

WORK DECK

The Chief Scientist or his designated representative will advise the Master of any sampling, launching,
streaming or  retrieval activity required.   The ship's cranes, winches, tools, rigging, etc. are to be
operated by ship's crew only; however, during extended operations, scientific personnel may be asked
to participate in these activities.

Scientific personnel should direct  all inquiries to  the Master, through the Chief Scientist, in matters
concerning work on deck,  navigation, safe working parameters, use of ship's equipment etc. Use of
this procedure will avoid working at cross purposes.

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When the ship is entering or leaving port, or during periods of poor visibility or high traffic activity,
and at the Master's discretion, the pilothouse should not be visited.  You may assemble on the 0-1
level (focsel) deck if you so desire.

While steaming during hours of darkness, no unauthorized lights should be displayed on deck.  This
is to comply with the Rules of the Road; lights shown forward of the deck house are detrimental to
the night vision of the conning officer.  While on station and engaged in sampling/test operations,
adequate night lighting will be provided.
LABORATORIES AND SCIENTIFIC EQUIPMENT

There are four laboratories located aboard the Lake Guardian:  chemistry lab (lower deck forward),
microbiology lab (main deck midship-starboard side), wet lab (main deck-forepart of deckhouse) and
container lab (boat deck-starboard side). The equipment and instruments contained in these labs are
primarily for support of the basic monitoring function of the ship. Some or all of this equipment may
be made available for use by guest scientists at the discretion of the U. S. EPA Project Officer or his
designated representative.
MESSING

Our cook prepares and serves varied and nutritious meals for our crew and the embarked scientific
party.  The small refrigerator below the salad bar is designated for your use during other than meals
hours.  Feel free to partake of the cold cuts, leftovers, fruit and soft drinks provided. Normal meal
hours are as follows:

                              Breakfast:     0730-0830
                              Lunch: 1130-1230
                              Dinner:        1700-1800

When large numbers of personnel are carried aboard, it is requested that meals be eaten comfortably
but quickly.  We have seating for 19 but must feed up to 43 personnel.

The stainless steel  refrigerator in the galley and the walk-in refrigerator and freezer contain food for
preparation of regular meals.  We request that you do not open these units, but rather ask the cook
for any item  which you cannot  find in the salad bar refrigerator.  We further request that any mess
gear used outside normal meal hours be scraped clean by the user and placed in the cart provided for
dishes and utensils to be  cleaned.

We request that the mess area be vacated by all  personnel 30 minutes before and after each meal in
order that the cook have  ample  space to prepare the  area for meals and to square away after meals.

Certain other amenities are expected and required:

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        1.      Appropriate clothing shall be worn to meals; this includes shirt and shoes.

        2.      Good breeding and shipboard protocol dictate that caps and other headgear be doffed
               when entering the mess area while the ship's company is at mess.

        3.      Late  arrivals  to meals due to  watch standing is expected,  however,  general
               nonconformity with the meal schedule is considered poor form. On occasion, ship's
               crew reporting for watch may be served ahead of someone who has been waiting.
               Since the crew must report on time, your consideration will  be appreciated.

        4.      When entering the  mess area for meals, neither space nor  sanitary considerations
               permit visits to the galley to look things over.  A menu is posted on the whiteboard.
               Take your dinnerware from the         dispensers provided and proceed to the salad
               bar and hot table and then to the seat of your choice.

        5.      The mess area is designated a no smoking area at all times.

        6.      During mealtimes, no food is permitted in the lounge.


SAFETY EVACUATION PROCEDURES

Ship evacuations will be conducted in accordance with the ship's station bill, as shown in Attachment
7.1 of the Safety, Health, & Environmental Safety Manual.
ALCOHOLIC BEVERAGES AND ILLEGAL DRUGS

Alcoholic beverages and illegal drugs in any form will not be consumed or brought aboard by any
persons.  Any personnel found to be in violation of this regulation will have the substance confiscated
and, upon arrival at the next port, will be discharged from the vessel.

FIREARMS

Firearms are prohibited on board the R/V Lake Guardian.


WASHING MACHINES AND DRYERS

Washing machines and dryers are provided and available for use by all personnel aboard.

The washing machines should only be  used when you have a full load; use for partial loads will
deplete the ship's fresh water supply. Use less than l/2 cup of detergent for a full load; additional soap
creates heavy sudsing which strains the machine and can leave residue in your clothing.  The washing
machines may be used while the ship is on station and collecting samples since the ship retains all
waste water.

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POTABLE WATER AND SEWAGE CAPACITY

The potable water and sewage holding capacity on board the Lake Guardian is severely limited.  The
showers, sinks, washing machine and toilets drain into a holding tank (for later transfer ashore).

The following guidelines are set forth to ensure conservation of resources:

        • Flush toilets only when required for human waste; all other disposable materials should be
         placed in a waste container.  Also,  please remain till the water has  stopped running in the
         toilet.  Any toilet, shower, or faucet that leaks must be reported immediately to the Bridge
         so it can be repaired.

        • When  showering,  please be brief;  the water supply is limited.   Each  shower head  is
         equipped with a mixing valve.  In  order to  control the water temperature turn the handle
         counterclockwise to increase the temperature and clockwise to decrease the  temperature.

        • Showers and washing machines can be used when on or approaching a sampling station.
         Low phosphate detergent is provided for  your use by the ship; do  not  use other types of
         soap in the washing machine.
CLEANLINESS

Cleanliness of the vessel is an "all hands" exercise.  Our limited crew size demands that each person
on board do his share to assist in maintaining clean, neat and orderly conditions.  Personnel assigned
to each space are responsible for the cleanliness of that space.  Public  areas of the vessel (heads,
passageways, mess area, etc.) are assigned  to members of the crew for cleaning.  Your cooperation
in this matter will be appreciated.
COMMUNICATIONS

Daily radio contact is made with the (ship's contractor's) office. The Master can handle routine or
emergency traffic via the marine operator as may be required.  Personal and business calls will be
made by credit card or collect only, unless prior arrangements have been made to pay for this service.

Use of the cellular phone, when activated and in a coverage area, is for government and business use
only. Permission to use same must be obtained from the Master or Chief Scientist and all calls must
be logged.
SMOKING

In an effort to maintain the enclosed spaces of the ship as "sweet smelling" as possible, we request
that smokers restrict this activity to outside areas.

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FUELING
Whenever the vessel is taking on fuel, as evidenced by the red "bravo" flag flying by day or by a red
light  on the mast at  night,  the  smoking  lamp  shall be extinguished throughout the vessel.
Announcements shall be made to further warn personnel when fueling operations are being conducted.
There shall be no smoking throughout the vessel.
STANDARD OPERATING PROCEDURES

A manual of Standard Operating Procedures (SOPs) is located in the main deck passageway.  Included
in this manual are procedures for performing certain functions aboard the vessel which could endanger
the safety of personnel or equipment. At their earliest opportunity, all scientific personnel should read
and become familiar with these procedures. Further, in the laboratory office is the U.S. EPA Region
5 Health and Safety Manual. Again, it is incumbent upon all scientific personnel to become familiar
with this manual and to abide by the requirements contained therein.
RESPONSIBILITY OF THE MASTER

The ship's Master (Captain) assumes responsibility for the safe operation and navigation of the ship.
He is responsible for the safety of all personnel on board and may curtail, delay or cease all operations
if, in his judgment, the vessel, personnel or equipment are  in jeopardy. The Master will confer and
cooperate with the Chief Scientist/Trial Director in making decisions at these times, and will comply
with the survey plan  so far as possible to achieve  the goals set forth therein.
TOURS OF THE VESSEL BY THE PUBLIC

The R/V Lake Guardian is funded by taxpayer dollars and an effort is made by U.S. EPA and (the
ship's contractor) to insure that the vessel is available to the general public on a not-to-interfere basis
with scientific operations, repair and maintenance work or crew privacy. With this in mind, scientific
and crew personnel desirous of providing interested parties with a tour of the vessel must first check
with the Master or the U. S.  EPA Project Scientist before conducting such a tour.  We request that
tours be conducted only during the hours of 0900-2000, with the exception of mealtimes.  Only public
areas (lab passageways, pilothouse, open deck areas) of the vessel may be shown.  Berthing areas are
off limits for tours, except for unoccupied cabins which may have open doors for viewing purposes
only.  Visitors should not enter the cabins.

This concludes our indoctrination procedure for our planned cruise. If we work as a team, everyone
does their job, and Mother Nature smiles upon us, we can expect another rewarding experience both
personally and professionally.

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Chanter 5 - PERSONAL PROTECTIVE EQUIPMENT

5.1  GLNPO PERSONAL PROTECTIVE EQUIPMENT POLICY
It  is the policy of GLNPO to  determine  the levels of protection required for  personnel assigned to
specific field or laboratory  operations and  to provide appropriate protective clothing and equipment, as
well as enforcing its  correct use.   Training  requirements, use  and control  of personal  protective
clothing, and equipment are further discussed  in Appendix I of this manual.  A Personal  Protective
Clothing and  Equipment Assignment Sheet  is included as Attachment 5.1.   Workplace  hazard
assessmements for all  GLNPO  operations, which include the types of personal protective equipment
required for recognized hazards, are included  in Attachment 5.2.  The  assessments are designed to
comply with the requirements of 29 CFR 1910  Subpart I.

Each year all GLNPO field and laboratory  personnel will be surveyed regarding existing health  and
safety equipment  inventory as well as any  additional requirements.  All GLNPO safety purchases must
be reviewed and  approved  by the  Region 5 Safety Manager.  Please also refer to Chapter 12 of the
U.S. EPA Region 5 Health  and  Safety Manual.

5.2 LEVELS OF PROTECTION

     I.  INTRODUCTION

     Response personnel must wear protective  equipment when  there is a probability of contact with
     hazardous  substances that  could affect  their health.  This includes vapors, gases, or participates
     that may be  generated  by  site activities, and direct contact with skin-affecting substances.  Full
     face piece respirators  protect  lungs,  gastrointestinal tract,  and  eyes against airborne  toxicants.
     Chemical-resistant clothing protects the skin from  contact with skin-destructive and absorbable
     chemicals.  Good  personal  hygiene habits prevent ingestion of material.

     Equipment to protect the body against contact with known or anticipated toxic chemicals has been
     divided into  four categories according to the degree of protection afforded:

        Level A:      Should be worn when the highest level of respiratory, skin, and eye  protection
                      is needed.

        Level B:      Should be worn when the highest level of respiratory protection  is needed,  but
                      a lesser  degree of skin protection  is needed.

        Level C.      Should  be worn when  a lesser  level of respiratory protection  is needed than
                      Level B. Skin protection criteria are similar to Level B.

        Level D:      Should be worn only as a work uniform and not on any  site with respiratory or
                      skin hazards. It provides no protection against chemical hazards.

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     The Level of Protection selected should be based on the hazard and risk of exposure.

        Hazard:       Type and measured concentration of the chemical  substance  in the ambient
                      atmosphere and its toxicity.

        Risk:         Potential for  exposure to substances  in air, splashes  of  liquids, or other  direct
                      contact with material due to work being done.

     In  situations where the type of chemical,  concentration,  and possibilities  of contact are  not
     known, the appropriate Level of Protection must be selected based on professional experience  and
     judgment until the hazards can be better characterized.

     Personal  protective  equipment  reduces the  potential  for  contact  with  toxic   substances.
     Additionally,  safe work  practices,  decontamination,  site  entry  protocols,  and  other   safety
     procedures  further ensure  the  health and  safety  of responders.   Together, these provide  an
     integrated approach for reducing harm to  response personnel.
II.   LEVELS OF PROTECTION

     A. Level A Protection

        1.     Personal Protective Equipment

                      Pressure-demand,  supplied-air  respirator  approved by  the Mine Safety and
                      Health Administration (MSHA) and National Institute for Occupational Safety
                      and Health (NIOSH).  Respirators may be:

                      1.      pressure-demand, self-contained breathing apparatus (SCBA), or

                      2.      pressure-demand,   airline  respirator  (with  an   escape   bottle  for
                              atmospheres with, or having the potential for, Immediately Dangerous
                              to Life or Health (IDLH) contaminant concentrations).

               •      Fully encapsulating chemical-resistant suit

               •      Coveralls*, or

               •      Long cotton underwear*

               •      Gloves (inner), chemical-resistant

                      Boots,  chemical-resistant,  steel  toe  and  shank.    (Depending  on suit
                      construction, worn over or under suit boot)

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                  Hard hat* (under suit)*

                  Disposable gloves and boot covers* (Worn over fully encapsulating suit)

          •       Cooling unit*

          •       2-Way radio communications (intrinsically safe)

          *  Optional

2.  Criteria for Selection

    Meeting any of these criteria warrants use of Level A Protection;

          •       The  chemical substance has been identified and requires the highest level of
                  protection for skin, eyes, and the respiratory system.

          •       Substances  with  a high  degree of hazard  to  the  skin are  suspected  to  be
                  present, and skin contact is possible.  Skin contact includes: splash, immersion,
                  or contamination from atmospheric vapors, gases, or particulates.

          •       Operations  must be conducted  in  confined, poorly  ventilated areas  until the
                  absence of substances requiring Level A protection is determined.

          •       Direct readings on  field Flame  lonization  Detectors (FID) or Photo  ionization
                  Detectors (PID)  and  similar  instruments  indicate high levels of unidentified
                  vapors and gases in the air.

3.  Guidance on Selection

    a.     Fully encapsulating suits are primarily designed to provide a gas or           vapor
          tight barrier  between the wearer and atmospheric contaminants.
          Therefore, Level A is generally worn when high  concentrations of airborne substances
          that could severely  affect the skin are known or presumed to be present.   Since  Level
          A requires the use of a  self-contained breathing apparatus, more protection is afforded
          to the eyes and respiratory system.

          Until air  surveillance data are available to assist in the selection of  the appropriate
          Level of  Protection, the  use of Level  A  may have to be based on indirect evidence of
          the  potential for atmospheric contamination or  other  means of skin  contact with
          substances having severe skin-affecting properties.

          Conditions that may require Level A protection include:

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•      Confined spaces:  Enclosed, confined, or poorly ventilated areas are conducive
       to build up of toxic vapors, gases, or particulates.
       An  entry  into  an enclosed space  does not automatically warrant  Level  A
       protection, but should serve as a cue to carefully consider the justification for a
       lower Level of Protection.

•      Suspected or known  highly toxic  substances:   Various  substances  that are
       highly  toxic,  especially through skin absorption, require  Level A Protection.
       Technical  grade  pesticides, concentrated  phenolic  compounds,  Poison  "A"
       compounds, fuming corrosives, and a wide variety of organic solvents  are  of
       this type.  Carcinogens,  and infectious substances known or suspected to be
       involved may require Level  A protection.   Field  instruments may not be
       available to  detect or quantify air concentrations of these materials.  Until
       these substances are identified and their concentrations determined, maximum
       protection is necessary.

•      Visible indicators:  Visible air  emissions from leaking containers or railroad or
       truck tank cars, as well as smoke from chemical fires and others, indicate high
       potential for concentrations of  substances that could  be extreme respiratory or
       skin hazards.

•      Job functions:    Initial  site entries  are  generally  walk-throughs  in  which
       instruments and visual observations are used to make a preliminary evaluation
       of the hazards.

In initial site entries, Level A  should be worn  when:

•      There is a probability for exposure to high  concentrations  of vapors, gases, or
       particulates.

•      Substances are  known or suspected of being extremely toxic directly  to the
       skin or by being absorbed.

Subsequent  entries   are  to   conduct   the  many   activities  needed   to   reduce  the
environmental  impact of  the  incident.  Levels of   Protection for  later operations are
based  not  only on  data obtained from  the  initial  and  subsequent  environmental
monitoring,  but also on  the protective  properties  of suit material  as  well.    The
probability of contamination and ease of decontamination must also be considered.

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          Examples of situations where Level A has been worn are:

          •       Excavating  soil  to  sample buried  drums  suspected  of containing  high
                  concentrations of dioxin.

                  Entering a cloud of chlorine to repair a valve broken in a  railroad accident.

          •       Handling and moving drums known to contain oleum.

                  Responding to accidents involving cyanide, arsenic, and  undiluted pesticides.

    b.     The fully encapsulating suit provides the  highest degree of protection to skin, eyes,
          and the respiratory system given that the suit material resists chemicals  during the time
          the suit is worn.  While Level  A provides  maximum protection, all  suit materials may
          be  rapidly permeated and degraded by certain chemicals. These limitations should be
          recognized when specifying the type of fully encapsulating  suit.  Whenever possible.
          the suit material should be matched with the substance it is used to protect against.

B.  Level B Protection

    1.     Personal Protective Equipment

          •       Pressure-demand,   supplied-air  respirator   (MSHA/NIOSH   approved).
                  Respirators may be:

                  1.      pressure-demand, self-contained breathing apparatus,  or

                  2.      pressure-demand, airline respirator (with escape  bottle for IDLH  or
                         potential for IDLH atmosphere).

          •       Chemical-resistant  clothing  (includes:   overalls and  long-sleeved  jacket  or
                  hooded, one  or two-piece chemical-splash suit or disposable  chemical-resistant,
                  one-piece suits)

          •       Long cotton  underwear*,  or

          •       Coveralls*

          •       Gloves (outer), chemical-resistant

          •       Gloves (inner), chemical-resistant

          •       Boots (outer), chemical-resistant, steel toe and shank

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              Boot covers (outer), chemical-resistant (disposable)*

              Hard hat (face shield*)

       •       2-Way radio communications (intrinsically safe)

       *  Optional

2.     Criteria for Selection

       Meeting any one of these criteria warrants use of Level B protection:

       The type and  atmospheric concentration  of toxic substances has been  identified  and
       requires a high level of respiratory  protection, but less  skin protection  than  Level A.
       These would be:

       •       Atmospheres with IDLH concentrations, but  the  substance or its  concentration
              in air does not represent a severe skin hazard, or

              Chemicals or concentrations  involved  do  not  meet  the selection    criteria
              permitting the use of air-purifying respirators.

       The atmosphere contains less than 19.5% oxygen.

       It is highly unlikely that the work being done will generate high  concentrations of
       vapors, gases or particulates, or splashes of material that  will affect the skin.

       Atmospheric concentrations  of unidentified  vapors  or  gases are indicated  by direct
       readings on instruments such as the FID  or PID or similar instruments, but vapors and
       gases are not suspected of containing concentrations of skin toxicants.

3.     Guidance on Selection

       a.      Level B does not afford the maximum skin (and  eye) protection  as does a fully
              encapsulating suit  since the chemical-resistant clothing is not considered  gas,
              vapor,  or particulate  tight.  However, a good quality,  hooded,  chemical-
              resistant,  one-piece garment, with taped wrist, ankles, and hood  does  provide a
              reasonable  degree  of  protection  against  splashes  of liquids  and  lower
              concentrations of chemicals in  the ambient air.

              At most abandoned, outdoor hazardous waste sites, ambient atmospheric gas or
              vapor levels usually do approach concentrations sufficiently high to warrant
              Level  A  protection.   In all  but a few circumstances, Level  B should provide
              the protection needed for initial reconnaissance.

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Subsequent operations require a re-evaluation of Level  B protection based on
the probability of being splashed by chemicals, their effect on the skin, or the
presence of hard-to-detect  air contaminants.  The generation of highly toxic
gases,  vapors, or  particulates, due to  the  work  being done,  must also  be
considered.

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          b.      The  chemical-resistant  clothing  required  in  Level B is available in a wide
                  variety of styles, materials, construction detail, and permeability.  One or two-
                  piece garments are available with or without hoods.   Disposable  suits with a
                  variety of fabrics and design characteristics are also  available.  Taping joints
                  between hood and respirator  reduces the possibility for splash and vapor or gas
                  penetration,  but is not a gas tight barrier.

                  These  factors and  other  selection  criteria  all affect  the degree of protection
                  afforded.  Therefore,  a specialist  should select the  most effective  chemical-
                  resistant clothing based on the known or anticipated hazards and job function.

                  Level B equipment does  provide a high level of  protection to the respiratory
                  tract.   Generally, if a self-contained  breathing  apparatus is required,  selecting
                  chemical-resistant clothing  (Level  B)  rather  than a  fully encapsulating  suit
                  (Level A) is based  on the need for  less protection  against known or anticipated
                  substances affecting the skin.  Level B skin protection is  selected by:

                  •      Comparing the concentrations of known or identified  substances in air
                         with skin toxicity data.

                  •      Determining the presence of substances that  are destructive to or  re-
                         absorbed through  the skin by liquid splashes, unexpected high levels of
                         gases, vapor,  or particulates, or by other means of direct contact.

                  •      Assessing  the  effect   of  the  substance   (at   its  measured   air
                         concentrations  or potential  for  splashing)  on  the  small areas  left
                         unprotected by chemical-resistant  clothing.  A hooded  garment, taped
                         to the mask with  boots and gloves taped to the suit, further reduces the
                         area for potential  skin exposure.

C.  Level C Protection

    1.     Personal Protective  Equipment

                  Air-purifying respirator, full-face, canister-equipped (MSHA/MOSH approved)

          •       Chemical-resistant  clothing (includes:  coveralls or hooded, one-piece or two-
                  piece chemical splash  suit or chemical-resistant  hood  and apron; disposable
                  chemical-resistant coveralls)

          •        Coveralls*,  or

           •        Long cotton underwear*

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•       Gloves (outer), chemical-resistant

        Gloves (inner), chemical-resistant

•       Boots (outer), chemical-resistant, steel toe and shank

•       Boot covers (outer), chemical resistant (disposable)*

        Hard hat (face shield*)

•       Escape mask*

•       2-Way radio communications (intrinsically safe)

*Optional

Criteria for Selection

Meeting all of these criteria permits use of Level  C protection:

•       Oxygen concentrations are not less than 19.5% by volume.

•       Measured  air concentrations  of identified  substances will be reduced  by the
        respirator  below  the substance's  threshold  limit   value  (TLV)  and  the
        concentration  is within the service limit of the canister.

•       Atmospheric contaminants, liquid splashes,  or  other direct contact will  not
        adversely affect any body left unprotected by  chemical-resistant clothing.

•       Job functions do not require self-contained breathing apparatus.

•       Direct readings are  a  few  ppms above  background on instruments such as the
        FID or PID.

Guidance on  Selection

a.       Level C protection  is distinguished from Level B  by the equipment used to
        protect the respiratory system, assuming the  same type of chemical-resistant
        clothing is used.  The main selection criterion for Level  C is that atmospheric
        concentrations   and  other  selection  criteria  permit  wearing  air-purifying
        respirators.

        The  air-purifying   device  must  be  a  full-face  respirator  (MSHA/NIOSH
        approved)  equipped with a canister suspended from the  chin or on  a harness.

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              Canisters  must be able to  remove the substances encountered.   Half-masks or
              cheek cartridge equipped, full-face masks should be used only with approval of
              a qualified  health  and  safety professional, preferably  a  Certified Industrial
              Hygienist.

              In addition, a full-face, air-purifying mask can be used only if:

              •       Substance has  adequate warning properties.

              •       Individual passes a qualitative fit test for the mask.

              •       Appropriate   cartridge/canister   is  used,   and  its   service   limit
                      concentration is not exceeded.

              •       Site  operations  are  not  likely  to  generate unknown  compounds or
                      excessive concentrations of already identified substances.

b.     An  air  surveillance program   is part of all  response  operations when  atmospheric
       contamination  is  known or suspected.  It  is  particularly  important that  the air be
       thoroughly monitored when personnel are wearing air-purifying respirators.   Periodic
       surveillance using direct-reading instruments and air sampling is needed to detect any
       changes in  air quality necessitating a higher level of respiratory protection.

c.     Level C protection with a full-face, air-purifying respirator should be worn routinely in
       an atmosphere  only after  the type  of  air  contaminant is identified,  concentrations
       measured and the criteria for  wearing an air-purifying  respirator met.   A decision on
       continuous wearing  of Level  C protection must be made after  assessing all  safety
       considerations,  including:

              •       The presence of (or potential for) organic or inorganic vapors  or gases
                      against which  a canister is ineffective or has a short service life.

              •       The known (or suspected) presence in air of substances with low TLVs
                      or IDLH levels.

              •       The presence of particulates  in air.

                      The  errors  associated with  both  the  instruments  and  monitoring
                      procedures used.

                      The  presence  of (or potential for) substances in air which do not elicit
                      a response  on  the instrument used.

              •       The  potential  for higher concentrations in the ambient atmosphere or in
                      the air adjacent to specific site operations.

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    d.     The continuous  use of  air-purifying  respirators  (Level  C)  must  be based  on  the
          identification  of the substances contributing to the total vapor or gas concentration and
          the  application of  published criteria  for  the routine  use of  air-purifying  devices.
          Unidentified ambient concentrations of organic vapors or gases in air approaching  or
          exceeding a few ppm above background require, at a minimum, Level B protection.

D.  Level D Protection

    1.     Personal Protective Equipment:

          •       Coveralls

                  Gloves*

          •       Boots/shoes, leather or chemical-resistant, steel toe and shank

          •       Safety glasses or chemical splash goggles*

                  Hard hat (face shield*)

          •       Escape mask*

          * Optional

    2.     Criteria for Selection

          Meeting any of these criteria allows use of Level D protection:

                  •       No contaminants are present.

                  •       Work  functions  preclude   splashes,  immersion,  or  potential  for
                         unexpected inhalation of any chemicals.

          Level D protection is primarily  a work uniform.  It  can be worn only in areas where
          there is no possibility of contact with contamination.
NOTE:   Levels C & D  are the most common levels  of protection used during GLNPO  field
          operations.

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III.  PROTECTION IN UNKNOWN ENVIRONMENTS

     In all incident response, selecting the appropriate personal protective equipment is one of the first
     steps in reducing health effects from toxic substances. Until the toxic hazards at an  incident can
     be identified and personnel safety measures commensurate with the hazards instituted, preliminary
     safety requirements must be based on experience, judgment, and  professional knowledge.

     Of  primary  concern  in  evaluating  unknown  situations  are atmospheric  hazards.   Toxic
     concentrations (or potential concentrations) of vapors, gases, and particulates, low oxygen content,
     explosive potential, and the possibility of radiation  exposure  all represent immediate  atmospheric
     hazards.  In addition to  taking air  measurements to  determine  these hazards, visual observation
     and  review of existing data can help determine the potential risks from  other materials.

     Once immediate  hazards, other   than toxic substances, have  been  eliminated, the initial on-site
     survey and reconnaissance continues.   Its purpose  is to further characterize toxic hazards  and,
     based on these findings, refine preliminary safety  requirements.  As  data is obtained  from the
     initial survey, the Level  of Protection and other safety procedures are adjusted.  Initial data also
     provide information  upon which to base further monitoring and sampling  requirements. No one
     method can determine a  Level of Protection in all  unknown environments.  Each  situation  must
     be examined individually.
IV.  ADDITIONAL CONSIDERATIONS FOR SELECTING LEVELS OF PROTECTION

     Other factors which should be considered in selecting the appropriate Level of Protection are:

     A.  Heat and Physical Stress

         The  use  of protective clothing and respirators increases physical stress, in particular,  heat
         stress, on the wearer.  Chemical protective clothing greatly reduces natural ventilation and
         diminishes the  body's  ability to  regulate its  temperature.    Even  in moderate ambient
         temperatures,  the diminished capacity of the body to dissipate heat can result in one or more
         heat-related problems.

         All  chemical protective  garments  can be a contributing  factor  to  heat stress.   Greater
         susceptibility  to heat stress occurs when protective clothing requires the use of a tightly fitted
         hood against the respirator facepiece, or when  gloves or boots are taped to the suit.  As more
         body  area is covered, less cooling takes place, increasing the probability  of heat stress.
         Whenever any  chemical-protective  clothing  is worn,  a heat stress  recovery  monitoring
         program  must occur.

         Wearing  protective equipment also  increases the risk of accidents.  It is  heavy,  cumbersome,
         decreases dexterity, agility, interferes  with  vision, and is  fatiguing to wear.  These factors  all

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                                                                 SECTION- CHAPTER 5
                                                                 VERSION:  FINAL/MAY 1997
                                                                 PAGE: 13 of 14
    increase  physical  stress and  the potential  for  accidents.   In particular, the  necessity of
    selecting Level  A  protection should  be balanced against the increased  probability of  heat
    stress and  accidents.   Level  B  and C protection  somewhat  reduces  accident probability
    because the equipment is lighter,  less cumbersome, and vision problems are less serious.

B.  Air Surveillance

    A  program must be established for routine, periodic air surveillance when Level  A  &  B
    situations are expected.  Without an air  surveillance program,  any atmospheric changes could
    go  undetected and  jeopardize  response  personnel.   Surveillance  can be accomplished with
    various types of air pumps and filtering devices followed by  analysis of the  filtering media,
    portable  real-time monitoring  instruments located strategically  on site;  personal dosimeters,
    and periodic walk-through  by personnel carrying  direct-reading instruments.

C.  Decision Logic for  Selecting Protective Clothing

    No adequate criteria,  similar to the respiratory  protection  decision-logic,  are  available for
    selecting protective clothing. A concentration of a known substance in the air approaching a
    TLV  or  permissible exposure  limit  for  the  skin  does  not  automatically  warrant a  fully
    encapsulating  suit.   A hooded, high  quality, chemical-resistant suit  may provide  adequate
    protection.   The selection of Level A over Level B is a judgment that should be made by a
    qualified individual (Certified Industrial  Hygienist) considering the hazards and risk.

    Hazards:       The  physical form of the  potential contaminant must be considered.  Airborne
                  substances are  more likely  to contact personnel  wearing non-encapsulating
                  suits, which are  not considered gas or vapor tight.  Liquids contacting the skin
                  are generally considered  more hazardous than  contact with vapors, gases and
                  particulates.
                  Effect of the contaminant on skin:

                  •       Highly hazardous substances are those that are easily absorbed through
                          the skin causing systemic effects, or that cause severe skin destruction.

                  •       Less hazardous  substances  are those  that  are not  easily  absorbed
                          through the skin causing systemic effects,  or  that do not cause severe
                          skin destruction.

    Risk:         Concentration of the contaminant: The higher the
                  concentration, the higher the probability of injury.

                  Work function:   Site work  activities dictate  the probability  of direct  and
                  indirect  skin contact.

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                                                                     SECTION: CHAPTER 5
                                                                     VERSION:  FINAL/MAY 1997
                                                                     PAGE: 14 of 14
                       Instability of the situation:  A higher Level of Protection should be considered
                       when there is a probability of a release  involving vapor or gases, splashes or
                       immersion in liquids, or through the loss of container integrity.

     D. Atmospheric Conditions

        Atmospheric conditions such as stability, temperature,  wind direction  and wind  velocity, as
        well as barometric pressure, determine the behavior of contaminants in air or the potential for
        volatile  material being released  into  the air.  These parameters should be considered  when
        determining the need for and Level of Protection required.

     E. Work in the Exclusion Zone

        For operations in the Exclusion Zone (area  of potential contamination),  different Levels of
        Protection may be selected,  and  various types of  chemical-resistant  clothing worn.  This
        selection would be based on measured air concentrations,  the job  function, the potential for
        skin contact or inhalation of the materials present, and ability to decontaminate the protective
        equipment used.

     F. Escape Masks

        Carrying an escape, self-contained breathing apparatus  of  at least  fifteen-minute  duration, is
        optional while wearing Level C or  Level D protection.   For  initial site entry,  a specialist
        should determine, on  a case-by-case  basis, whether they should  be  carried, or be  strategically
        located in areas that have higher possibilities for harmful exposure.
V.   Vapor or Gas Concentrations As Indicated by Direct-Reading Instruments

     Instruments such as the FID and PID can be used to detect the presence of many organic vapors
     or gases either as single  compounds  or  mixtures.   Dial readings are  frequently  referred to,
     especially with  unidentified  substances, as  total vapor and gas  concentrations (in ppm).   More
     correctly, they  are deflections of the needle  on  the dial indicating an instrument response and do
     not directly relate to the total concentration  in the air.

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                                         SECTION: CHAPTER 5, ATTACHMENT 5.1
                                         VERSION: FINAL/MAY 1997
                                         PAGE: 1 of 1
Attachment   5.1  -  PERSONAL  PROTECTIVE  CLOTHING   AND  EQUIPMENT
ASSIGNMENT FOR GREAT LAKES NATIONAL PROGRAM
Employee:,
       Social Security Number:_
Work Operation:,
                           Phone:
Yes
a
a
a
a
a
a
a
a
a
Other
No
a
a
a
a
a
a
a
a
a




Respiratory Protection (specify)
Protective Eye Wear, Normal
Protective Eye Wear,
Face Shield
Hard Hat
Laboratory Coat
Gloves, Chemical
Safety-toe Shoes
Rubber boots


Prescription








Supervisor
Date
GLNPO Safety Manager/Regional Safety Manager

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                                       SECTION: CHAPTER 5, ATTACHMENT 5.2
                                       VERSION:  FINAL/MAY 1997
                                       PAGE: 1 of 1
Attachment 5.2 -  PERSONAL PROTECTIVE EQUIPMENT ASSESSMENTS

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                                   US EPA REGION 5
                  "WORKPLACE HAZARD ASSESSMENT AND CERTIFICATION41

                            WORKPLACE HAZARD ASSESSMENT

 TiPE OF INSPECTION:  MARINE LAB AND SAMPLING  RV LAKE GUARDIAN and
                       RV HYDRA
 EMPLOYEES AFFECTED:  Great Lakes  National Programs  Office
                          Surveillance and Research Staff
 WORKPLACE EVALUATED:  Surveillance,  Research  and other Field Activities
                        Aboard Research Vessels
 HAZARD SOURCE

 IMPACT
                         HAZARD POTENTIAL

                         Yes; flying and
                          falling otHecta
RECOMMENDED PP8

Glasses w/side shields/ goggles or
faceahield; hardhat;  steel-toed footwear,
 PENETRATION
                         Yes; sharp objects   Puncture-resistant gloves.
 COMPRESSION
 (Rollover!
                         No
 CHEMICAL
                         Yes; wide & varied
                          range of potential
                           chemical exposure
Latex gloves; lab coat or apron; and other
 equipment,  as outlined in the Chemical
 Hygiene Plan.	
 HARMFUL DUST
                         Yes; nuisance and
                          chemical dusts
                          exposure potential
Latex gloves; lab coat or apron; and other
 equipment,  as outlined in the Chemical
 Hygiene Plan.	
 LIGHT  (Optical) RADIATION  No
 ELECTRICAL ENERGY
                         Yes; work in wet
                          locations
Insulating mats and gloves.
 IMMERSION IN WATER
                         Yes; during  survey
                          cruises
Fall protection, life preserver 6/or survival
 suit, and boat.           	
       DEFICIENCY
                         No
TtuSRMAL EFFECTS
RADIATION
NOISE
No
Yes; C,<
Yes; ship engines

Glasses w/side shields; lab coat or apron.
Ear plugs
FALLS
                         Yes
Fall protection (full body harness,
 restraint belt),  as needed.
	                       ,          .	
NOTE: It  is the responsibility of the  supervisors and employees to reassess
       the workplace hazard situation,  as necessary, by  identifying and
       evaluating hazards,  other than those identified above,  and prescribe
       appropriate personal protective  equipment.
                        CERTIFICATION OF HAZARDS  ASSESSMENT

I certify  that the workplace hazards,  routinely and non-routinely encountered
by  US EPA  Region 5, during MARINE LAB  and SAMPLING aboard RV LAKE QOARDIAM
• ad/or RV  HYDRA, employees and for which Personal Protective Equipment has
been provided,  have been  assessed for  the determination of  Personal
Protective Equipment appropriateness,  in compliance with 29 CFR 1910 Subpart
I.
   s  assessment has been  conducted by A.  James Finn, US EPA  Region 5 Safety,
   1th and  Environmental  Management Program Manager.
                                                 Date

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                                  US EPA REOION 5
                  "WORKPLACE HAZARD ASSESSMENT AND  CERTIFICATION"

                            WORKPLACE HAZARD ASSESSMENT

TYPE OF  INSPECTION: MUD  POPPY SEDIMENT SAMPLING
EMPLOYEES  AFFECTED: Great Lakes National Programs Office
                             Remedial Programs  Staff

WORKPLACE  EVALUATED: Surveillance,  Research  and other  Field Activities
                       Aboard Research Vessels
HATXRO SOURCE

IMPACT
HA2ARD POTENTIAL

Yes;  flying and
 falling objects
RECOMMENDED PPB

Glasses w/side shields,  goggles or
faceeh^eld; hardhat? steal-toed footwear.
PENETRATION
Yes;  sharp objects  Puncture-resistant gloves.
COMPRESSION
(Rollover!
No.
CHEMICAL                 Yes; wide & varied   Nitrile or neoprene gloves; MSA 1/2 or full-
                          range of potential   face respirator w/GMC-H canister.
                 	chemical exposure	
HARMFUL OUST              Yes; nuisance and   Nitrile or neoprene gloves: MSA 1/2 or full-
                          chemical dusts      face respirator w/GMC-H canister.
			exposure potential	 	     	
LIGHT (Optical) RADIATION  No.
ELECTRICAL ENERGY
Yes; work in wet
 locations	
Insulating mats and gloves.
IMMERSION IN WATER
Yes.
Fall  protection,  life preserver &/or survival
 suit, and boat.	
r~YCBN DEFICIENCY
No.
THERMAL EFFECTS
No.
RADIATION
No.
NOISE
                         No.
FALLS
Yes
Fall protection (full body harness,
 restraint belt), aa needed.	
NOTE: It is the responsibility  of the supervisors and employees  to reassess
       the workplace hazard situation, as necessary, by identifying and
       evaluating hazards, other than those identified above, and prescribe
       appropriate personal protective equipment.
                        CERTIFICATION OF HA2ARD8 ASSESSMENT

I certify that  the workplace hazards, routinely and non-routinely  encountered
by  US EPA Region 5, Great Lakes  National Programs Office Remedial  Programs
Staff,  employees and for which Personal Protective Equipment has been
provided, have  been assessed for the determination of Personal Protective
Equipment appropriateness,  in compliance with  29 CFR 1910 Subpart  I.

      assessment has been conducted by A. James Finn, US EPA Region 5  Safety, ^|
        and Environmental Management Program Manager.                         ^H
          y^^jj^
    .Tames 'Finn
                        Ddte

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                                  US EPA  REGION 5
                  "WORKPLACE HAZARD ASSESSMENT AND CERTIFICATION1'
  -YPE OF INSPECTION:

 EMPLOYEES AFFECTED:
 WORKPLACE EVALUATED:
 HAZARD SOURCE

 IMPACT
     WORKPLACE HAZARD ASSESSMENT

Wetlands t, all other Water-relate^  (la.  small
boat, vadina, «tc.)  Activities
Water Division and  CDO,  EDO & CRL  (Env'l Sciences Div)
Enforcement, Research,  Inspections  and other Field
Activities at Host  Employer Sites
  HAZARD POTENTJM,

  Yes;  flying and
  falling objects
RECOMMENDED PPB

Glasses w/side shields, goggles or
faceshield; hardhati ateal-toed boots.
 PENETRATION
  Yes; sharp objects
Puncture-resistant gloves.
COMPRESSION
{Rollover)
CHEMICAL
HARMFUL DUST
NO
NO
No
 LIGHT  (Optical) RADIATION  No
 ELECTRICAL ENERGY
 Yes; work in damp
  locations
Rubber boots
 IMMERSION IN WATER
 Yes; innersion in
  water potential
Fall protection; life preserver, &/or
 waders.	
 OXYGEN DEFICIENCY
 NO
 THERMAL EFFECTS
 No
    IATION
 No
 NOISE
 Yes;  sources  from       Ear plugs
  eguipment &  operations
 FALLS
                         No
 NOTE: It  is the responsibility  of  the supervisors and  employees to reassess
       the workplace hazard  situation,  as necessary, by identifying and
       evaluating hazards, other than  those identified  above,  and prescribe
       appropriate personal  protective equipment.
                        CERTIFICATION OF HAZARDS ASSESSMENT

I certify that  the workplace hazards,  routinely and non-routinely encountered
by  US EPA Region 5 employees, conducting WETLANDS INSPECTIONS,  and for which
Personal Protective Equipment has been provided, have been  assessed for the
determination of Personal Protective Equipment appropriateness,  in compliance
with 29 CFR  1910 Subpart I.

This assessment has been conducted by  A.  James Finn, US EPA Region 5 Safety,
    lth and Environmental Management Program Manager.
A. Jamtffl Finn
                        Date
      \j u

-------
                                  US EPA REGION 5
                  "WORKPLACE HAZARD ASSESSMENT AND CERTIFICATION"
      OF  INSPECTION:
 EMPLOYEES AFFECTED:
 WORKPLACE EVALUATED:
HAZARD SOURCE
 IMPACT
                  WORKPLACE HAZARD  ASSESSMENT                         M

             GENERAL INDUSTRIAL INSPECTIONS
             Air t Radiation,  Waste Management, and  Water Divisions;
             CDO, EDO, PTSB  &  MQAB  (Env'l  Sciences Div)  and all other
             Branches and Offices within Region 5, as  needed
             Enforcement Inspections and other Field
             Activities at Industrial Sites,  including laboratories
              HAZARD POTENTIAL

              Yea; flying and
               falling objects
RECOMMENDED PPB

Glasses w/side shields, goggles or
  faceahield; hardhat;steel-toed footwear.
 PENETRATION
              Yes; sharp objects
Puncture-resistant gloves.
COMPRESSION
(Rollover)
              No.
CHEMICAL
              Yes; wide 6 varied
               range of potential
               chemical exposure
Nitrile or neoprene gloves; MSA 1/2 mask
 or fullface respirator w/GMC-H canister.
HARMFUL DUST
              Yes; nuisance, chemical
               & asbestos dusts
               exposure potential
Nitrile or neoprene gloves; MSA 1/2 mask
 or fullface respirator w/GMC-H canister.
LIGHT (Optical) RADIATION  No
ELECTRICAL ENERGY
              No
IMMERSION IN WATER
              No
OXYGEN DEFICIENCY
              No
.DERMAL EFFECTS
              NO
RADIATION
              NO
NOISE
              Yes;  in manufacturing   Ear plugs
               & industrial settings	
FALLS
              Yes
Fall protection (full body harness,
 restraint belt),  as necessary.	
NOTE:  It  is  the responsibility  of  the supervisors  and employees to reassess
       the workplace hazard situation, as necessary,  by identifying and
       evaluating hazards, other than those identified above,  and prescribe
       appropriate personal protective equipment.
                        CERTIFICATION OF HAZARDS ASSESSMENT

I certify  that the workplace hazards,  routinely  and non-routinely encountered
by  US EPA  Region 5 employees, conducting GENERAL INDUSTRIAL  INSPECTIONS,  and
for which  Personal Protective Equipment has been provided, have been assessed
for the determination of Personal  Protective  Equipment appropriateness,  in
compliance with 29 CFR 1910 Subpart I.

This assessment has been conducted by A. James Finn,  US EPA  Region 5 Safety,
Health and Environmental Management Program Manager.
A. Ja
Finn
  Date //

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                                                          SECTION: CHAPTER 6
                                                          VERSION: FINAL/MAY 1997
                                                          PAGE'l of 3
Chapter 6 - MARINE SAFETY
6.1  SHIP OPERATION SAFETY

GLNPO  research vessels shall  be regularly  inspected to  minimize potential marine safety
hazards.  A formal inspection must be conducted on a  monthly basis by GLNPO personnel or
designee  who  have a working  knowledge of OSHA  standards and regulations,  as  well  as
familiarity with marine safety operations.  Research vessel operations shall be conducted in
accordance with OSHA standards found in  29 CFR  1910 and 29 CFR 1926  as  well  as
applicable standards  found in 46 CFR  and 33 CFR.  A  semi-annual  inspection  will be
conducted by the Regional Health and Safety Manager.   Inspection requirements appear in
Appendix V of this manual.

Overall  standards  for research  vessel operation safety must  be developed  by  the ship's
operations contractor and  documented in a safety manual.  At  a  minimum, the following
minimum safety requirements should  be addressed:

      •      Safety and Fire Protection During Welding & Burning Operations
      •      Inspections of  Standing and Running  Rigging,  Cargo  Handling  Gear,  and
             Scientific Equipment Overboarding Systems
      •      Lockout / Tagout  of Equipment under Repair
      •      Pre and Post Sail Electrical Equipment Checkout
      •      Fueling Procedures
      •      Annual Vessel Inspections
      •      Fixed Electrical Space  Heaters
      •      Maintenance of Emergency Equipment
      •      Small Boat Operational Safety
      •      Deck Safety Practices
6.2 SHIP PERSONNEL SAFETY

All personnel aboard GLNPO  research vessels will be required to comply with  the health.
safety, and environmental regulations in accordance with the Environmental Health and Safety
Compliance  Manual  for U.S.  EPA  GLNPO Vessels.  Research  vessel employees  will be
trained in the areas of health, safety, environmental compliance, and fire prevention.  Training
requirements appear in Chapter 2 of this manual.

In addition to the requirements specified in the Environmental Health and Safety Compliance
Manual for U.S.  EPA GLNPO Research Vessels, health and safety protocols are also  specified

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                                                            SECTION- CHAPTER 6
                                                            VERSION: FINAL/MAY 1997
                                                            PAGE:2 of 3
in Appendix O,  Policy, Memorandums,  Standard Operating Procedures, and Regulatory
Updates.  The requirements specified  in these two documents are designed to enhance the
overall  health, safety,  and environmental compliance  program for GLNPO research  vessel
operations.

6.3 NEW EMPLOYEE ORIENTATION

GLNPO research  vessel  laboratory  personnel  are required to  receive  laboratory  safety
orientation training.   The  safety  orientation will contain  rules  for  safe  operations  in
laboratories, fire evacuation and alarm locations, waste disposal, spill control stations, personal
protective equipment, and location of eyewashes.

GLNPO research vessel personnel not working in  laboratory areas will  also be required to
receive  safety orientation  training.  The safety orientation will include general  safety rules,
fire evacuation plans and  emergency response procedures, and exposure to potential hazards.
Training requirements are  detailed in Chapter 2 of this manual.

6.4 FIRE SAFETY

All occupants  aboard  GLNPO  research  vessels  are  required to participate  in fire  safety
training exercises and drills.  All personnel are expected to be familiar with the operational
procedures identified in  the  Occupant  Emergency Plan  developed for the vessel.   The
Occupant Emergency Plan appears as Appendix M of this manual.

6.5 FIRST AID AND CPR

First Aid/CPR training  and certification is required for specific personnel who work aboard all
GLNPO research vessels.  For specific training requirements in First Aid and CPR, refer to
Chapter 2.1.3.

6.6 OCCUPANT EMERGENCY PLAN

The  Federal  Government is responsible for  minimizing  danger  to  life and property  arising
from  the  effects of  bomb threats, bombs, enemy attack, fire, explosion,  earthquake,  serious
weather  disturbance, civil disturbance, and  other  disasters  affecting  federal property.   In
response to this, the  General Services Administration requires the development of an  Occupant
Emergency  Plan.  The Occupant Emergency Plan for GLNPO research vessels appears as
Appendix M of this manual.

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                                                           SECTION: CHAPTER 6
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE:3 of 3
6.7 CONFINED SPACE ENTRY

Confined Space Entry Policies and Procedures for GLNPO personnel are detailed in Chapter
2 and Appendix E of this manual.

6.8 VISITOR SAFETY

From time  to time, GLNPO research vessels will conduct "open houses" where visitors may
board and tour the vessels.  During these times, research vessel personnel will be responsible
for the health and safety of the visitors.  For v^sel- specific visitation policies, please refer to
Attachment 6.1 of this chapter. Visitor evacuation procedures and visitor security procedures
can be found in Chapter 7, Sections 7.5. and 7.6, respectively.

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                                                   SECTION.  CHAPTER 7
                                                   VERSION: FINAL/MAY 1997
                                                   PAGE: 1 of 3
Chapter 7 - EMERGENCY PREPAREDNESS AND RESPONSE

OVERVIEW

It  is  the policy of GLNPO  to comply with  all applicable  federal,  state and  local
regulations regarding emergency  preparedness and response.  This policy  is not limited to
hazardous material  spills  or  the transportation of hazardous materials, but  extends to
firefighting operations, contractor support operations, as well as evacuation plans and
security procedures for "open houses" aboard research vessels.


7.1    HAZARDOUS MATERIAL SPILLS -RCRA, OSHA

In the  event of a hazardous material spill the Hazardous Waste Contingency Plan will be
implemented  immediately.   The contingency plan contains  specific  procedures to  be
carried out in  the event of a release.  An example of a Hazardous Material Contingency
Plan for GLNPO can be found as Appendix R of this  manual.


7.2    HAZARDOUS MATERIAL TRANSPORTATION

It  is the policy  of U.S. EPA  Region  5 to  comply with all applicable regulations when
transporting hazardous materials by government  vehicle.   Appendix S  of this  manual,
entitled Procedures for the Transportation of Hazardous Materials/Waste by Great Lakes
National Program  Office  VIA  Government Vehicle  and  Government  Driver, details
necessary regulations and  appropriate packaging  for the  transportation  of hazardous
materials.  GLNPO  will comply with the regulatory  and  policy  requirements specified
within  this document.


7.3    FIRE FIGHTING OPERATIONS

Fire prevention  programs must  be  established in all  GLNPO facilities and  aboard  all
research vessels  to reduce the possibility of fire.  If a fire does occur, well-developed fire
fighting procedures will be implemented.  Aboard research vessels, the captain is in charge
of  fire prevention  and  protection.   He will  establish  inspection schedules,   provide
recordkeeping. and  ensure that all fire fighting equipment is properly inspected and in
working order.  Fire equipment inspections will include such items as fire  pump operation.
fire hydrants, sprinkler systems, water  supply, portable  fire extinguishers, fire doors, exits,
and detectors.  A research vessel fire  inspection program is described in  Appendix  V of
this manual.

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                                                    SECTION: CHAPTER 7
                                                    VERSION:  FINAL/MAY 1997
                                                    PAGE: 2 of 3
Response teams will be available 24-hours a day  and regularly scheduled training for  all
members of the team will be conducted.  At least one hour a week will be scheduled for a
response training session.  These sessions will include proper procedures on how to avoid
fire, hands-on training  for drills  including signals and individual responsibilities, as well as
procedures for the prevention of bodily injury.   A portion of this training will be devoted
to the practice of emergency exit drills to ensure  that all exits  are clearly marked, easily
passable, and that all alarms are in working order.   Any deficiencies noted during drills or
regular inspections will be corrected immediately.

Emergency  signal   instructions  and  personnel assignments  for  research  vessels  are
contained in the "Station Bill"  which appears  as  Attachment 7.1 of this chapter.   An
operational diagram for research vessels appears as Attachment 7.2 of this Chapter.
7.4    INJURY. ILLNESS, OR MEDICAL EMERGENCY

GLNPO has retained medical consultation services of a maritime medical advice service.
In the event of an injury or illness, the Captain or his designee will contact the service for
interim  medical advice for treatment of ship  board injury  or illness  that is within the
medical capabilities of on-board personnel.  The contact information for these  services can
be found as Attachment 7.4 of this chapter.  Additionally, the medical contractor  shall
supply a document that contains complete contact and follow up information, as well as,
supporting documentation.

In the  event  anyone aboard  a ship  at  sea becomes  ill  or  is injured beyond  the  clear
capability of the ship to provide prompt and adequate care,  a Medical Emergency (ME)
exists and that person should be transferred to  a facility providing emergency medical
treatment.

If the Captain (or officer in charge) determines that a possible ME exists, the U.S. and/or
Canadian Coast Guard should be contacted for advice  and assistance.  The Coast Guard
may be hailed on  radio Channel  16 throughout all the Great Lakes.  The Coast Guard can
provide  medical  consultation and  evaluation  of the  situation  and  are  equipped for
evacuation by boat or helicopter, if needed.

Always  contact the Coast  Guard  for  advice.   Not all  ports have emergency medical
facilities and not all  may be suitable for a particular ME.

These  procedures and  numbers should  be readily available for all  employees  aboard
GLNPO research vessels.

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                                                  SECTION: CHAPTER 7
                                                  VERSION:  FINAL/MAY 1997
                                                  PAGE: 3 of 3
Telephone  numbers for Coast  Guard areas and  designated trauma centers  appear as
Attachment  7.5 of this chapter.
7.5    CONTRACTOR SUPPORT OPERATIONS

All outside  contractors  will  comply  with health, safety,  and environmental  policies
according to Federal, State, and Local regulations.
7.6    EVACTT\TION  PLAN  FOR  VISITORS  ABOARD  RESEARCH VESSELS
       DURING AN OPEN HOUSE

In the  event of a fire or other  alarm  incident during open house, the  Personnel/Visitor
watches must direct visitors to the nearest safe exit leading to the gangplank. The watch
must then proceed with response activities.
7.7    SECURITY ABOARD RESEARCH VESSELS DURING AN OPEN HOUSE

Prior to an open house, a "Pre-open House Check List" will be completed to ensure that
the ship is  safe  and secure  for  visitors to come aboard.   The  checklist  appears  as
Attachment 7.3 of this  chapter.   Also, during an open house, vessel personnel will  be
assigned to a personnel/visitor watch at  designated locations throughout the ship.  The
personnel/visitor watch will  prevent  visitors from  entering  restricted areas  as  well  as
monitor and  prevent  unsafe  activity.   Uncooperative  visitors will  be reported to  the
Captain (1st or 2nd Mate) who will escort the visitor ashore.

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                                               SECTION:  CHAPTER 7, ATTACHMENT 7.1
                                               VERSION:  FINAL/MAY 1997
                                               PAGE: 1  of 3
Attachment 7.1  - STATION BILL
SIGNALS

FIRE AND EMERGENCY- Rapid ringing of the ship's bell and continuous ringing of general
alarm bells for a period of at least 10 seconds.
ABANDON SHIP - More than  6 short blasts and  1 long blast  on the  whistle and the same
signal on the general alarm bells.
MAN OVERBOARD - Hail, and pass the word "Man Overboard" to the bridge.
DISMISSAL - From FIRE AND EMERGENCY stations, 3 short blasts on the whistle and 3
short rings on the general alarm bells.
INSTRUCTIONS

I.    Immediately upon reporting on board, the entire crew shall familiarize themselves with the
     location of their emergency stations and the specific duties required at that station.

2.    Each  crew member shall be provided  with an individual  supplementary station bill  card
     which must show, in detail, the special  duties to be performed.

3.    The entire crew shall be instructed in the performance of their special duties and the crew
     on watch will remain prepared to  receive the signal for an emergency drill.

4.    Every person  participating in  the  abandon ship drill  will  be  required  to  wear a  life
     preserver.  The entire boat crew shall assist in removing the chain rails.

5.    Immediately upon receiving the emergency  signal, the Emergency  Squad will assemble
     with the appropriate equipment at the scene of action.

6.    The Steward's department will assemble and direct passengers to  embarkation stations.

7.    The first person to discover a fire shall immediately  notify the bridge and fight  the  fire
     with available equipment provided that life and health are not immediately jeapordized.

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                                               SECTION: CHAPTER 7, ATTACHMENT 7.1
                                               VERSION:  FINAL/MAY 1997
                                               PAGE: 1 of 3
8.    Immediately upon receiving the FIRE  AND EMERGENCY signal,  fire pumps will be
     started, all watertight doors, ports, and air shafts will be closed, and all fans and blowers
     stopped.  The fire hose will be deployed in the affected area.

9.    Upon hearing the signal, "MAN OVERBOARD", life ring buoys will be thrown overboard,
     the engines stopped, and  a lookout  sent aloft.   The  Emergency Boat Crew shall
     immediately clear the rescue boat for launching.

10.  During periods  of low visibility, all watertight doors and ports below the bulkhead deck
     shall be closed, subject to the Master's orders.

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                                                           SECTION'  CHAPTER?, ATTACHMENT 7.1
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE: 1  of 3
R/V LAKE  GUARDIAN CREW  ASSIGNMENTS DURING  FIRE,  EMERGENCY AND ABANDON SHIP
INCIDENTS
NO.  RATING

1    Master


2    Chief Mate

3    Second  Mate

4    Chief Engineer

5    Asst. Engineer

6    Electrician


7    Able Seaman

8    Able Seaman

9    Orel  Seaman

10   Cook

11   Steward

12   Elect Tech

13   Marine  Tech

14   Chief Scientist

15   Scientist

16   Scientist

17   Scientist

18   Scientist

19   Scientist

20   Scientist

21   Scientist

22   Scientist

23   Scientist

24   Scientist
FIRE & EMERGENCY STATIONS

On Bridge-In Overall Command
Emergency

At Scene, in Charge-Assist Firemen

Provide Fog Applicator-Assist Firemen

Engineroom - Start Fire Pump

Engineroom - Assist Chief as Directed

Make Ready Portable Fire Pump-
 Assist

At Scene-Provide  Extinguisher & Axe

At Scene-Provide  SCBA, Man Flose

On Bndge-At Helm

At Scene-Provide  Air Pack, Man Hose

At Scene-Provide  Extinguisher, Assist

Man Portable Fire/De-vvatering Pump

At Scene-Don Firesuit, Man  Hose

O-2 Deck Aft-Muster Scientists

O-2 Deck

O-2 Deck

O-2 Deck

O-2 Deck

O-2 Deck

O-2 Deck

O-2 Deck

0-2 Deck

O-2 Deck

O-2 Deck
ABANDON SH1P-EIFERAFT STATIONS

In Command-Provide Nav


Launch Raft-Provide Compass

Launch Raft-Provide Hand Held Radio

Launch Raft

Assist Launching of Raft

Direct Personnel to Rafts 1 & 3


Assist Launch or Raft

Launch Raft

Direct Personnel to Rafts 2 & 4

Provide First Aid Kit

Provide Blankets

Assist Launch of Raft

Assist Launch of Raft

O-2 Deck Aft-Muster Scientists

O-2 Deck

O-2 Deck

O-2 Deck

0-2 Deck

O-2 Deck

O-2 Deck

O-2 Deck

O-2 Deck

O-2 Deck

0-2 Deck

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                                             SECTION:  CHAPTER 7, ATTACHMENT 7.2
                                             VERSION:  FINAL/APRIL 1997
                                             Att. 7.2 - Page 1
                              BRIDGE
                     (Alarms, Pumps, Ventilation)
          Port Team
 Fireman:
Fireman:
Provide air pack and
assist hose
Fireman:
Fire exting., axe, and
assist hose
                            FIRE SCENE

                            Chief Engineer

                              Engineer
Starboard Team
                                                  Fireman:
                                                 Fireman:
                                                                Crewman on Pumps:
                                                                 Crewman on Alarms
     Provide air pack and
     assist hose
                                                 Fireman:
     Fire exting., axe, and
     assist hose

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                                              SECTION:  CHAPTER 7, ATTACHMENT 7.3
                                              VERSION:  FINAL/MAY 1997
                                              PAGE. 1 of 7
Attachment 7.3 - PRE-OPEN HOUSE CHECK LIST FOR R/V LAKE GUARDIAN

• Yes or No

Preliminary:
 Y      N
 a      a         Visitor (school, press, VIP) schedule review by tour guide.

 a      a         Sanitary holding tank low enough to preclude  drain back-up  in  wet lab
                 floor.

 a      a         Pedestrian walkways and water-edge railings adequate.

 n      n         Mooring lines not obstructing walkway.


Boarding Area:
 Y      N
 n      n         Welcome sign, times and dates.

 n      n         Welcome sign secure.

 n      n         Trash  can available ashore.

 n      n         Gangplank - on-shore step.

 n      n         Gangplank secured against lateral movement.

 n      n         Gangplank - railings.

 D      D         Gangplank - walk surface.


Main Deck Aft:
 Y      N
 n      n         Gangplank access aboard unobstructed.

 n      a         Removable gate  railing secured against falling.

 n      n         Fantail deck cleared of loose equipment and oil spills.

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                                               SECTION- CHAPTER 7, ATTACHMENT 7.3
                                               VERSION: FINAL/MAY 1997
                                               PAGE: 2 of 7
Y     N
 n     n        Knuckle crane secured and off.

 n     D        Aft A-frame secured and off.

 a     a        Starboard A-frame and winch secured and off.

 n     n        Engine room hatch closed.

 n     D        Main deck starboard side at rear of containers taped against entry.

 n     n        Main deck port side clear of obstructions.

 n     n        Boat hook available under aft container lab.

 a     a        Life rings in place - fantail.

 n     n        Counting  device  in  place (may need  a second  one  to count  visitors
                 disembarking).

n      n        Guide to R/V Lake Guardian tour brochures and stickers at hand on deck.
Container Labs:
 Y    N
n     n         Aft sea door open.

o     n         Hazardous Materials Container locked (lights on?).

n     n         Container  labs cleared of hazardous materials, lights on, doors  open but
                 taped to prevent entry.
Wet Lab:
 Y     N
a      n         Port sea door open.

n      n         Wet lab lights on.

n      a         Video tape rewound, brochures, guest book and pen, press kits ready.

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                                                SECTION:  CHAPTER 7, ATTACHMENT 7.3
                                                VERSION:  FINAL/MAY 1997
                                                PAGE: 3 of 7
 V     \
n      a         File drawers closed/locked.

o      a         All counters cleared of chemicals, samples, glass, except for
                 display/demonstration items.

o      a         Floors clear of slip/trip hazards.

n      a         Refrigerator clear of hazardous materials and locked.

a      a         Hood cleared out and closed.

a      a         Non-potable water turned off.

D      D         Hazardous material drains closed.

a      a         Computer off or set to prevent tampering; all disks secure.

n      a         Pilferable items stowed.

D      d         Posters in place.

D      a         Double sea doors open.

n      a         Rosette sensors/controls off and secured

n      a         Rosette lowered to  rack  (winch controls off and secured).

n      a         A-frame secured and off.

3      D         Gunwale safety chain in place.

n      a         Sampling deck chained or taped against entry.

n      a         Equipment displays in place.
Passageway:
 Y    N
o     Q         Both hallway aft sea doors open.

n     O         Walk-in refrigerator locked.

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                                               SECTION: CHAPTER 7, ATTACHMENT 7.3
                                               VERSION: FINAL/MAY 1997
                                               PAGE: 4 of 7
Y    N
n     n         Exercise room locked.

n     a         Posters in place.

n     n         Chem. lab  cleared of hazardous  material, lights  on,  doors  open, taped
                 against entry.

D     n         Bio lab cleared of hazardous material, green lights on,  doors open, taped
                 against entry.

n     n         Hall fire door closed, sign saying "Entry Permitted".

a     n         Lab office door locked (due to hit/strike hazard of fire door opening into a
                 person entering/leaving lab office).

n     n         Scientist  cabin,  clean, no valuables/pilferables,  open without taping,  (If
                 occupied, should be locked).
D     o         Restrooms clean, lights on, toilet paper and paper towels available.

n     n         Starboard equipment/utility room locked.

D     n         Laundry room clean; no filled laundry baskets,  lights on, door open.

Galley/Lounge:
 Y     N
a     a         Video tape rewound.

a     a         Water fountain clean.

n     a         Refrigerators locked.

a     a         All food stuffs put away.

n      n         Paper cups put away.

a     a         Soda dispenser turned off.

n     n         Storage cabinets locked.
4

-------
                                                SECTION: CHAPTER 7, ATTACHMENT 7 3
                                                VERSION:  FINAL/MAY 1997
                                                PAGE. 5 of 7
Y      N
o      n         Floors clean.

D      n         Engine room door closed, "No Entry" sign in place.

Focsle Deck:
 Y     N
Q      o         Passageway lights on.

n      n         Forward two fire doors closed, "Entry Permitted" signs in place.

o      n         Mechanical room door locked.

a      a         Crew's quarters locked.

n      a         Office locked.

n      n         All occupied EPA and Scientist quarters locked.

n      a         Two vacant quarters open, lights on, pilferable articles stowed.

n      n         Water fountain clean.

D      n         Sheath on fire ax pick.

n      n         Aft door latched open, weather permitting.

n      n         Focsle aft deck clear of trip/slip hazards.

Q      D         Winches secure and covered.

n      a         Access to  container tops taped to prevent entry.

a      n         Both paint lockers closed and locked.

n      a         Bow deck taped to prevent access to anchor equipment.

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                                               SECTION:  CHAPTER 7, ATTACHMENT 7.3
                                               VERSION:  FINAL/MAY 1997
                                               PAGE: 6 of 7


Bridge Deck;
 Y    N
o     n         Dog house locked.

n     n         Battery racks secured against jettison.

n     n         Inflatable boat cover secure.

n     o         Trip hazards in area of boat davits marked with warning tape.

n     n         Safety chain at boat davit gunwale in place.

n     n         Ladders marked with warning tapes  to prevent access to rigging.

n     n         Both emergency gear (SCBA, etc.) lockers closed, but not locked, and taped
                 against access.

D     n         Radar turned off and secure.

n     n         Wing bridges off, secure and covered.

Bridge:
 Y    N
n     n         Bridge doors unlocked and open, weather permitting.

a     n         Pilferable bridge items stowed.

a     n         Rudder controls off and secure.

a     a         Cellular phone activated and/or shore telephone located.


Personnel:
 Y    N
 n     n         EPA and Crew visitor duty station.

 n     a         Gangplank Ashore: seaman (optional).

 a     3         Fantail/gangplank:  seaman.

 a     a         Wet Lab: contractor  or EPA Scientist and Public Affairs.

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                                               SECTION: CHAPTER 7, ATTACHMENT 7 3
                                               VERSION: FINAL/MAY 1997
                                               PAGE: 7 of 7
 Y    N
a     a         Lounge:  seaman (optional).

n     a         Focsle aft deck: seaman.

o     n         Bridge Deck:  seaman (optional).

n     D         Bridge:  Ship's Officer.

o     n         School Tour Guide: Science Representative.

o     a         Press (et al) Host: Science Representative/Public Affairs.

o     a         EPA Science Representative.


After Tour;
 Y    N
n     o         Check all decks and unlocked spaces for stragglers/stowaways/damage.

a     n         Close gangway.

n     a         Change welcome sign, time, date.

a     a         Enter visitor count in log.

a     a         Rewind video tapes.

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                                                 SECTION:  CHAPTER 7, ATTACHMENT 7.4
                                                 VERSION:  FINAL/MAY 1997
                                                 PAGE: 1  of 1
Attatchment 7.4      Medical Advice Services Contractor

The EPA has contracted Maritime Medical Access (MMA) of The George Washington University Medical
Center to provide medical  advice services.  The service is available for four vessles; OSV  Peter W.
Anderson, Lake Explorer, R/V Lake Guardian and R/V Mudpuppy.  This is a 24 hour per day service.
Medical advice shall  be  provided by the  emergency room attending physican at George Washington
University Medical Center.

Communication Information:

Telephone (Maristat):   (202)994-0473
                     (202)994-5644

Telex:                GWU Med   496086888

PLAD:               GWU MEDCEN WASHINGTON DC

Facsimile:            GW Maritime Medical (202)994-0962

Single Sideband:      Any available coast station

George Washington University Medical Center
2140 Pennsylvania Avenue,  N.W.
Building VV
Washington, DC 20037
Phone:  (202)994-3921
Fax:    (202)994-3924

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                                                      SECTION:  CHAPTER 7, ATTACHMENT 7 5
                                                      VERSION:  FINAL/MAY  1997
                                                      PAGE- 1  of 6
Attachment 7.5
                                       MARINE MEDICAL

                                 EMERGENCY OPERATIONS PLAN

                                  U. S. C. G. Group:  Great Lakes

In the event anyone aboard a ship at sea becomes ill or is injured beyond the clear capability of the ship to
provide prompt and adequate care, a Medical Emergency (ME) exists and that person should be transferred to
a facility providing emergency medical  treatment.

If the Captain (or  officer in charge) determines that a possible ME exists, the U.S. and/or Canadian  Coast
Guards should be contacted for advice  and assistance.  The Coast Guards may be hailed on radio Channel 16
throughout all the  Great Lakes.  The  Coast Guards can provide medical consultation and evaluation of the
situation and are equipped for evacuation by boat or helicopter, if needed.  Always contact the Coast Guard for
advice. Not all ports have emergency medical facilities and not all may be suitable for a particular ME.

Additionally,  GLNPO has procured the emergency consultation services of George Washington Hospital.  In
the event of an emergency, the Captain or his designee will contact the service for interim medical  advice for
the treatment of ship board injury or illness.  For complete communication information see Attachment 7.4.

These procedures and telephone numbers should be  readily  available to all employees  aboard our research
vessels.

2.5.1  Coast Guard Areas
Coast Guard Command  Center (Cleveland)
Anywhere in the Great Lakes
(800)321-4400
(216)522-3984
Note: The (800) number  will only work within the area of the Great Lakes, outside of this area use the (216)
number.  The Command Center can contact the any Coast Guard station within the Great Lakes.
Buffalo
Lake Ontario & East '/2 of Lake Erie
Coast Guard: (716)843-9500
Health Services:  (716)846-4167
Trauma Centers:  Mercy Hospital, Buffalo, NY
                (716) 828-2790
                Sisters of Charity, Buffalo. NY
                (716)862-1810
Sault St. Marie
Lake Superior and Upper 1/3 of Lake Huron
and Upper 1/3 of Lake Michigan
Coast Guard:  (906)635-3233
Health Services: (906)635-3225
Trauma Centers:   Marquette   Gen.,   Marquette,
                 MI   (800)562-9753   or
                 (906)228-9440
                 Northern Michigan  Regional,
                 Petosky, MI
                 (616)348-4000

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                                                     SECTION: CHAPTER 7, ATTACHMENT 7.5
                                                     VERSION:  FINAL/MAY 1997
                                                     PAGE: 2 of 6
Detroit
West !/2 of Lake Erie and Lower 2/3 of Lake Huron
Coast Guard:  (313) 568-9524
Health Services: (313)568-9526
Trauma Centers:  Detroit Receiving, Detroit, Ml
               (313) 745-3356
               Henry Ford, Detroit, MI
               (313)641-4029

Milwaukee
Western '/•> of Lak*. Michigan
Coast Guard:  (414)747-7181
Health Services: (414)747-7111
Trauma Centers:  Froederct General Hospital
                (414)259-3000
    Grand Haven
    Eastern Vz of Lake Michigan
    Coast Guard: (616)847-4501
    Health Services: (616)847-4542
    Trauma Centers;:  Ottawa County Hospital
            (616)847-5310
2.5.2  Great Lakes Regional Hospitals


 ILLINOIS
 Chicago

 Northwestern Memorial Hospital
 Superior St. &  Fairbanks Court
 Chicago, IL 60611
 (312)908-2000
 Ask  for Emergency Rm.; 5-10 min. from harbor.

 Columbus  Hospital
 2520 N. Lakeview Avenue
 Chicago, IL 60614
 (312)883-7300
 5-10 minutes from Lake; unsure of distance from
 harbor.
Zion

Midwestern Regional Medical
Emmaus &  Shiloh Boulevard
Zion, IL 60099
(847)872-4561
Unknown distance from harbor
one in area.
There is more than
 INDIANA

 Gary

 Methodist Hospitals Northwest Indiana
 600 Grant Street
 Gary, IN 46402
 (219)886-4000
 20 miles from harbor; 15 minutes.
Hammond

St. Margaret Hospital
5454 Hohman Avenue
Hammond, IN 46320
(219)933-2077

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                                                    SECTION: CHAPTER 7, ATTACHMENT 7.5
                                                    VERSION:  FINAL/MAY 1997
                                                    PAGE: 3 of 6
MICHIGAN

Arcadia (See Frankfort)

Charlevoix

Charlevoix Area Hospital
Lakeshore Drive
Charlevoix, MI 49720
(616)547-0027
1 mile from harbor.

Escanaba

St. Francis Hospital
3401 Ludington Street
Escanaba, MI 49829
(906)786-3311
2 miles from harbor.

Frankfort

Paul Oliver Memorial Hospital
224 Park Avenue
Frankfort, MI 49635
(616)352-9621
Half mile from harbor.

Grand Haven

N. Ottawa Community  Hospital
1309 Sheldon Road
Grand Haven, MI 49417
(616)847-5310
2 blocks from harbor.

Holland

Holland Community Hospital
602 Michigan Avenue
Holland, MI 49423
(616)394-3202
10-15 minutes from harbor.

Harbor Springs (See Petoskey)

Leland (See Frankfort or Traverse City)
Ludington
Memorial Medical Center
One Atkinson Drive
Ludington, MI 49431
(616)845-2390
I  mile from harbor.

Manistee

West Shore Hospital
1465 E. Parkdale
Manistee, MI 49660
(616)723-3501 Ext. 150
5 miles from harbor.

IVIanistique

Schoolcraft Memorial Hospital
500 Main Street
Manistique, MI 49854
(906)341-2163
3 blocks from harbor.

Montague/Whitehall/Muskegon

Hackley Hospital
1700 Clinton Street
Muskegon, MI 49443
(616)726-3511
3 miles from  harbor.

Mercy Hospital
1700 Oak Avenue
Muskegon, MI 49442
(616)739-9341
10 miles from harbor.

Pentwater (See  Ludington)

Petosky
Northern Michigan Hospital
416 Connable Street
Petosky, MI 49770
(616)348-4520
10 miles from harbor.

Portage Lake (See Manistee)

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                                                 SECTION:  CHAPTER 7, ATTACHMENT 7.5
                                                 VERSION: FINAL/MAY 1997
                                                 PAGE: 4 of 6
MICHIGAN (CONTINUED)

South Haven
South Haven Community Hospital
955 S. Bailey Avenue
South Haven, MI 49090
(616)637-5271 Ext. 0
MINNESOTA
Duluth
St. Mary's Emergency Care Trauma Center
407 E. Third Street
Duluth, MN 55805
(218)726-4357
Traverse City

Munson Medical Center
1105 Sixth Street
Traverse City, MI 49684
(616)922-9000
5 minutes from bay.

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                                                   SECTION: CHAPTER 7, ATTACHMENT 7.5
                                                   VERSION:  FINAL/MAY 1997
                                                   PAGE: 5 of 6
WISCONSIN

Gill's Rock (see Strugeon Bay)

Green Bay

St. Mary's Medical Center
1726 Shawano Avenue
Green Bay, WI 54303
(414)498-4560
1 hour from bay.

Kenosha

Kenosha Hospital
6308 Eighth Avenue
Kenosha, WI 53140
(414)656-2201
5-8 blocks from harbor.

Kewaunee

St. Mary's Memorial
First & Lincoln
Kewaunee, Wf 54216
(414)388-2210
5 minutes or less from harbor; about 2 miles.

Manitowoc

Holy Family Medical Center
2300 Western Avenue
Manitowoc, WI 54221
(414)684-2011
21 blocks frori Lake.

Milwaukee

St. Luke's Medical Center
2900 W. Oklahoma Avenue
Milwaukee, WI 53215
(414)649-6000

Port Washington

St. Mary's Ozaukee
743 N. Montgomery Street
Port Washington, WI 53074
(414)243-7373
Racine

St. Mary's Medical Center
3801 Spring Street
Racine, WI 53405
(414)636-4201
5-10 minutes from harbor.

St. Luke's Memorial
1320 Wisconsin Avenue
Racine, WI 53403
(414)636-2201
j-10 minutes from harbor.

Sheboygan

Sheboygan Memorial Medical
2629 N7 7th  Street
Sheboygan, WI 53083
(414)451-5000

St. Nicholas Hospital
1601 N. Taylor Drive
Sheboygan,"wi 53081
(414)459-8300
3-4 min. from harbor.

Sturgeon Bay

Door County Memorial
330 S.  16th"Place
Sturgeon Bay, WI 54235
(414)743-5566
5-10 miles from harbor.

Two Rivers

Two Rivers community Hospital & Hamilton
Memorial Home
2500 Garfield Street
Two Rivers, WI 54241
(414)793-1178
6 blocks from Coast Guard Station.

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 CANADA
                                                   SECTION: CHAPTER 7, ATTACHMENT 7.5
                                                   VERSION:  FINAL/MAY 1997
                                                   PAGE: 6 of 6
 ONTARIO
 Thunder Bay

 Port Arthur General Hospital
 460 N. Court St.
 Thunder Bay, Ontario P7A4X6
 (807)343-6621
 5-10 minutes from  Marina.
Welland Canal

Wetland Co. General Hospital
Third Street
Welland, Ontario L3B4W6
(905)732-6111 Ext. 3250
30 minutes from Lake Ontario.
*NOTE: All hospitals listed provide 24-hour Emergency Room Service with a physician on duty.

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                                                            SECTION: CHAPTER 8
                                                            VERSION:  FINAL/MAY 1997
                                                            PAGE: 1 of 4
Chapter 8 - ENVIRONMENTAL COMPLIANCE
Executive Order 12088, signed by President Jimmy Carter in 1978, requires Federal Agencies
to comply with all requirements of federal, state, and local environmental regulations.  As
such,  EPA Regions must comply with these regulations concerning air, water, hazardous and
nonhazardous  waste, toxic substance control, emergency planning, and community right-to-
know.   Additionally,  all U.S. EPA  employees,  including  U.S. EPA contractors, as  well  as
their representatives, are required to  act in an environmentally responsible manner to protect
themselves, the public, and the environment.

Acting in accordance  with Executive Order 1^088, each department and division of the U.S.
EPA  is required to establish practices and procedures which  comply  with these regulatory
requirements as they relate to individual U.S. EPA work operations.  As a division  of U.S.
EPA Region 5, GLNPO and GLNPO research  vessels must have well established programs
for ensuring  compliance  with  federal,  state, and  local  regulations  regarding  releases  of
hazardous and non-hazardous substances into the environment.  GLNPO personnel, therefore,
must  comply  with the regulations detailed in  the  Toxic  Substance  Control Act and the
Resource  Conservation and Recovery Act and understand  how these regulations affect their
work  operations.
8.1  TSCA - PCBs

The  Toxic Substance Control Act (TSCA)  primarily applies to manufacturers,  distributors.
processors,  and importers  of chemicals.   This law specifically bans  the manufacture of
polychlorinated biphenyls (PCBs).  All federal facilities, however, who use  or dispose of any
PCB items are  subject to TSCA regulations specified  in Section 6 of the TSCA regulations as
they appear in  40 CFR Part 761.  Since  GLNPO  research laboratories  and vessels use and
dispose of samples, standards, and  stock solutions  containing  PCBs,  they are  required to
comply with  TSCA  through proper  PCB  labeling,  storage,  dilution,  spill cleanup,  and
disposal.
8.2  RCRA

The  Resource Conservation and Recovery Act (RCRA) of 1976 was enacted to ensure proper
identification, reporting, transporting,  storage, and disposal of hazardous and non-hazardous
solid waste.   Section 6001 of RCRA requires that all Federal Agencies be in compliance with
this  act.  In  the case  of non-hazardous solid waste, Federal facilities are required to comply
with the regulations specified in 40 CFR Parts 240-249.  For hazardous solid waste. Federal
agencies are required to comply with the regulations specified in 40 CFR Parts 260-272.

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                                                            SECTION: CHAPTER 8
                                                            VERSION:  FINAL/MAY 1997
                                                            PAGE: 2 of 4
8.2.1  SOLID NON-HAZARDOUS WASTE HANDLING. STORAGE. AND DISPOSAL

GLNPO facilities and research vessels generate three profiles  of non-hazardous solid waste.
These waste profiles  are  considered as 1) trash  designated for a land disposal facility,  2)
broken glassware, and 3)  recyclable cans and paper.  GLNPO facilities and research vessels
must, therefore, dispose  of their non-hazardous solid waste in compliance with 40 CFR Parts
240-249.  These regulations  specifically relate to regulatory compliance by the waste disposal
facility and to recycling.

Trash Designated for a Land Disposal Facility

Trash that cannot be recycled must be placed in garbage cans conveniently located throughout
GLNPO office  facilities and on board  GLNPO research vessels,  including laboratory areas.
Examples of such  trash  include laboratory towels,  labware  (excluding  glass) that  is not
contaminated, and solid samples that are not designated as hazardous waste.

Recyclable Waste

GLNPO personnel,  as well as GLNPO contractors,  are required  to participate  in a recycling
program for newspapers, white paper, uncontaminated glassware and aluminum cans. Broken,
uncontaminated glassware is also considered recyclable. Recycling  containers,  such as trash
cans, must be conveniently located throughout GLNPO offices and research vessels.

Hazardous Waste

As  amended, RCRA  regulates  the generation,  treatment, storage, and disposal  of hazardous
waste from "cradle to grave"  and all Federal  agencies are required to comply with these
regulations as  they appear in 40  CFR  Parts  260-272.   Individual  states,  however,  are
authorized to  operate hazardous  waste disposal  programs in lieu  of the federal program.
Based on this authorization, GLNPO  facilities and  GLNPO research vessels are required  to
comply  with State regulations when disposing of hazardous waste.

Waste Minimization

GLNPO facilities  and research vessels are specifically encouraged to minimize  hazardous
waste generation where  possible.  Minimizing  the generation  of  hazardous  waste  reduces
potential hazards to employees, the public, and the environment.

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                                                           SECTION- CHAPTER 8
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE. 3 of 4
Trainin»
Federal and State regulations require annual training for personnel who  will be  generating,
handling,  and disposing  of hazardous waste.   GLNPO  is  required to provide this  initial
training to personnel whose work operations involve hazardous waste.  The training must be
designed to familiarize personnel with hazardous  waste handling procedures and emergency
response actions.  Annual refresher training must be provided thereafter.
8.3 DRINKING WATER

The U.S. EPA requires that EPA facilities monitor drinking water for lead and copper levels.
If these  levels reach or exceed  the  action level,  then  appropriate action  must be taken.
GLNPO  complies  with this policy through the monitoring of drinking fountains and sink
faucets, and bottled water used as  sources of drinking water in GLNPO facilities and aboard
GLNPO research vessels.
8.4 GLNPO ENVIRONMENTAL COMPLIANCE MANUAL

GLNPO  facilities and  GLNPO  research  vessels  are  committed  to  complying  with  all
applicable rules and regulations in an effort to serve as a model to other U.S. EPA programs
in the  areas  of environmental health and  safety.  GLNPO has developed an environmental
compliance  manual which outlines environmental  compliance strategies and procedures to
ensure that health and safety is a constant and consistent  priority in the work operations at
GLNPO  facilities and aboard  GLNPO research  vessels.   This environmental  compliance
manual appears as Appendix T of this manual.

8.5 AIR EMISSIONS AND TOXICS

Rules and regulations pertaining to air emissions and toxics are specified  in Appendix T,
Chapter 2, Air.
8.6  INDOOR AIR QUALITY

Indoor air  quality measurements and surveys are conducted as  part of the  annual  industrial
hygiene survey for GLNPO  research vessel laboratories and work areas.  Reports of findings
are provided to the U.S. EPA Region 5  Safety and Health Manager, and any deficiencies are
corrected under his direction.

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                                                           SECTION: CHAPTER 8
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE: 4 of 4
8.7 ASBESTOS PRECAUTIONS
The U.S. EPA National Policy Statement for Asbestos-Containing Materials (No. SHEMD-92-
001) specifies the requirements  for the identification and management  survey of asbestos-
containing materials in U.S. EPA facilities.  Depending on asbestos survey results for GLNPO
facilities and research vessels, an Operations  and Maintenance  Plan may be  developed to
address management of asbestos-containing materials in accordance with SHEMD policy.

The  policy  and Standard Operating Practices  used in  the  SPIEMD  plan were  developed
through the Safety, Health, and  Environmental Management  Divisions National Technical
Services Center,  using  the  "Policy and  Standard Operating  Practices Manual for  the
Management of Asbestos-Containing Building  Materials at EPA  Facilities" (Third  Draft,
March 1992).

Asbestos surveys  will  be performed  for  all  GLNPO  facilities  and  research  vessels in
accordance with SHEMD-92-001. Results of inspections for specific facilities and vessels are
located in Appendix U of this manual.

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                                                         SECTION:  CHAPTER 9
                                                         VERSION-  FINAL/MAY 1997
                                                         PAGE- 1 of 6
Chapter 9        HEALTH AND SAFETY PROCEDURES DURING GLNPQ SEDIMENT
                  SAMPLING OPERATIONS

9.1     BACKGROUND

The Sediment Assessment Program is  designed to sample and evaluate areas of sediment where the
possibility of contamination exists.  During sampling, field specialists and laboratory analysts may
be exposed to sediment which represents a chemical or biological hazard.  Therefore,  the potential
for  exposure should  always be of  foremost  concern  when handling  sediments of  unknown
contamination.

GLNPO sediment sampling activities are grouped into the following five categories:

    1.  Sediment sampling at Great Lakes Harbor
    2.  Tributary sediment monitoring
    3.  Open lake sediment monitoring
    4.  Support for EPA regions for Superfund and enforcement  activities
    5.  Miscellaneous activities

Sediment  assessment and tributary monitoring may entail  collecting long core and grab samples
with low  to high amounts of contamination.  Typical sediment assessments  span six to twenty
days.   Operation  of all R/V Mudpiippy equipment is usually  necessary.  Open lake  sediment
monitoring is generally conducted aboard the R/V Lake Guardian and involves the use of heavier
equipment where the  captain  is responsible for navigation and positioning of the vessel.

Sediment  sampling to support EPA regional offices or other governmental agencies provides the
largest degree of diversity in sampling  activities. Sampling  operations  range from use of all or
part of the R/V Mudpuppy equipment  to  field-flexible sampling plans  based  on observations of
samples and/or their environments.  Most of GLNPO's sediment  sampling activities, however, will
be performed on  board the  R/V Mudpuppy either on an outdoor work bench  or  in  a  mobile
laboratory.  Refer to Appendix N and Appendix O of this manual for  safety protocols for the
R/V Mudpuppy.

Sampling  aboard the  R/V Mudpuppy  will include one U.S. EPA/GLNPO technical individual  and
two contractors.  Personnel will have  experience  in the operation of small craft vessels, as well as
in the use of sampling equipment.  Additional staff may participate in sediment sampling activities
provided they meet the training requirements described in the GLNPO safety manual.
9.2    SAFETY

Prior to commencing field operations, the field team should gather all site data available and assess
the anticipated hazard level of sediments to be sampled.  When assessing the sediments' hazard
potential  for a site, the depth sampled below the surface must always be considered.  For example.

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                                                           VERSION: FINAL/MAY 1997
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if all historical data available for a site comes from  Ponar dredged samples, all  samples collected
below the sampling depth of the Ponar should be treated as unknown and potentially hazardous.
Refer to Appendix A and  Appendix  I  of this  manual  for  additional  information  regarding
Respiratory Protection and Personal Protective Equipment.

       A.  Personal Protection  for Contaminated Sediment

       This section examines the procedures that will be  implemented by all personnel working
       with contaminated  sediment.   It should be noted that  research conducted with  sediment
       varies  considerably depending on the scope and objective of the research.  Therefore, the
       guidelines set  forth in this SOP may not  be  applicable to all  situations  dealing  with
       potentially contaminated sediments.

        1.      Medical Surveillance.   Health monitoring  will be provided for personnel working
               with sediments.  Health monitoring establishes a baseline to which all subsequent
               medical monitoring can be compared.  All field  and laboratory personnel must be
               monitored prior to working with any  sediments.

       2.      Personal  Precautions.   Workers  must  always  be aware  of possible  points  of
               contamination as described by the supervisor.  Hands should always be kept away
               from the eyes and mouth. After completion of a manipulation involving sediment
               or the  removal of potentially contaminated clothing (gloves, protective  clothing,
               etc.), the hands, forearms, and  other areas of possible contact  should be washed
               with  soap and  water.   Do  not use organic solvents to  clean  the  skin.  These
               solvents may increase penetration of the contaminant into the skin.

       3.      Clothing.  When  working with  sediments, it is  of  the utmost importance to avoid
               skin contact.  A fully fastened, knee-length lab coat must be worn in the laboratory
               work  area  at  all  times.   Disposable Tyvek®  lab clothing must  be  worn for
               sediment manipulation.   During field operations,  disposable Tyvek® or Saranex
               suits must  be worn by  all  personnel who may have possible  contact  with the
               sediment.

        4.      No shorts or skirts are to be  worn when working with hazardous materials.

        5.      Hand  Protection.   Hands will be the most frequent point of potential contact with
               contaminants.   Gloves  must be worn to avoid skin contamination.   Disposable
               gloves must be discarded after each  use  in appropriate containers designated for
               this use.  Double gloves will be used with the outer glove being stripped off after
               any potential exposure.  Torn or punctured gloves must be discarded and replaced
               immediately.   Rubber,  latex  or  vinyl  gloves do  not provide full protection.
               Contaminants  may diffuse into the  gloves.  When  sediment  is handled, gloves
               should be changed frequently.  Cuffs must be tight fitt'ng or taped to the sleeve to
               prevent inward migration of contaminants.

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                                                    SECTION- CHAPTER 9
                                                    VERSION.  FINAL/MAY 1997
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6.       Eye Protection. Safety glasses must be worn at all times.  In addition,  face shields
        will be made available in the work area.  On deck, face  shields will  be  required
        when hauling coring equipment and sediment cores until the sediment is extruded
        from the core tube and deemed, when possible, non-hazardous.

7.       Further  Precautions.   Protective  disposable  footwear is  recommended  during
        sediment  manipulation.  Long  hair should be tied back and loose clothing should
        be  covered.  Eating,  drinking,  smoking, chewing  gum, and  smokeless  tobacco are
        prohibited in  the work  area.    In  addition, respirators  may  be required when
        sediment  is  manipulated.   Respirators will  be stored in an  appropriate  manner
        when not in  use

B.  Facility Engineered Protection

    The following guidelines are for the laboratory work area where
    sediments will be tested.

    1.      Area identification and access control.

           a.     The laboratory work area where  sediments are used or stored  will  be
                  properly identified.  Access to the designated laboratory work area will
                  be limited.  Laboratory  doors will be kept closed.
           b.     Animals and plants not related to the  analytical procedure shall not be
                  permitted in the laboratory.

    2.      Eyewash stations  and hand washing  facilities are  available  in the laboratory
           work  area.

    3.      Contaminant  Devices.    Work  with sediment  will  be  performed  in  an
           appropriate  containment device.  Procedures  involving  sediment will  not  be
           conducted  on an open  bench  due to  the potential hazard for  generating
           contaminated dusts, aerosols, or fumes.  Hoods and ventilated work areas are
           used  to   minimize the  worker  exposure  to contaminants  associated  with
           sediment.   All   containment devices will be constructed  out  of  smooth,
           unbreakable material,  such  as Teflon®, stainless steel,  polyethylene, fiberglass,
           or plexiglass.  Exhaust air from hoods or a ventilated work area does not have
           to be  filtered but it must be discharged out of the  building, as far from the air
           intake supply as  possible.

    4.      Equipment. Use of instruments such as pH, dissolved  oxygen or  conductivity
           meters will be used in a  ventilated work area or hood.  This equipment will be
           enclosed in plastic to reduce the potential for  contamination or kept  outside
           the hood on a lab cart.   Instruments will be serviced or  calibrated in the work
           area.   All calibration and  maintenance  log  books should be kept  with  the
           equipment.    All equipment  that  has   come in  contact   with  potentially

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                                                           SECTION: CHAPTER 9
                                                           VERSION: FINAL/MAY 1997
                                                           PAGE: 4 of 6
                  contaminated  sediment must be  kept  either under  negative pressure (e.g., a
                  hood) or sealed in an  air-tight container (e.g., a Tupperware container) before
                  it is cleaned.

           5.      Work  Surfaces.     All  laboratory  work  surfaces  potentially  exposed  to
                  hazardous  sediments must  be covered  with Teflon® sheets, plastic trays, dry
                  absorbent  plastic-backed  paper,  foil,  or  other  impervious  or  disposable
                  material.   If a surface  becomes contaminated  or  a spill occurs, the work
                  surface should be decontaminated or disposed of immediately.

           6.      Housekeeping.  The laboratory  work  area shall!  be kept clean  and  orderly.
                  Clean up shall follow every operation or, at  a  minimum, at  the end of each
                  day.  Containers for disposal of contaminated materials will be placed in the
                  work area.

           7.      Spill Control.   A  hazardous sediment  spill will be treated  as  a  "Chemical
                  Spill": Organic Solvent".   The sediment  spill  will  be  contained  with the
                  appropriate absorbent material.
                  If a spill occurs, the worker should:

                  (a)   pour  absorbent material on the spill quickly,  using  enough material to
                       absorb all fluid and cover the mass with excess dry absorbent to control
                       vapors;

                  (b)   sound the air horn to signal for help if necessary;

                  (c)   if problems are encountered in containing the spill, consideration should
                       be given  to evacuating the building,  routing personnel away from the
                       problem area;

                  (d)   clean  up absorbents and dispose of them properly as hazardous waste;

                  (e)   when safe, allow  personnel  to return to the laboratory  work area.

9.3    STORAGE OF SEDIMENT

Solid-phase sediment and sediment extracts will be stored at  4°C in air-tight  containers in the dark.
Proper identification and sample  tracking information must accompany  all  samples.  Sediment
extracts can be temporarily stored at  4°C  in  designated  lab refrigerators.   On  board, sediment
samples are stored inside coolers in either capped core  tubes or in sample containers.  All samples
are to be  transported   from  the refrigerator  to work  area  on a lab cart in sealed containers.
Sediment  will  alwavs  be transferred  using  double  containment.  Transfer  of sediment from the
storage container  is  a   procedure which involves a  potential  hazard for  personal  contamination.
During this procedure,  the number of investigators in the laboratory work area should be kept to a
minimum.

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                                                        SECTION:  CHAPTER 9
                                                        VERSION:  FINAL/MAY 1997
                                                        PAGE: 5 of 6
9.4    HOMQGEN1ZATION  AND PREPARATION OF ELUTRIATE SAMPLES

Mixing and sampling of solid-phase sediment or sediment extracts  will be done in the  original
storage container under a hood. If the containers holding sediment are removed from the hood, an
intermediate non-breakable  container must be used.  The worker must use a respirator with organic
vapor-acid  gas filters and  appropriate clothing as described in Chapter 6 of this manual  when
solid-phase sediment or sediment extracts are not under a hood or in a ventilated work area.
9.5    CLEAN UP OF EQUIPMENT AFTER SEDIMENT PROCESSING

Glassware and equipment coming  in contact with sediment will be cleaned as soon as possible.
Cleaning glassware poses an increased exposure hazard.  Therefore, all glassware must be cleaned
under the vented sinks or hoods located in the laboratory work area.

9.6    SEDIMENT ASSESSMENT FIELD OPERATIONS SAFETY STANDARD
       OPERATING PROCEDURES

The following rules must be followed by sediment assessment field personnel:

                                SMOKING IS NOT ALLOWED.

       1.     When away from the dock, a personal flotation device must be worn any time you
              are out of the cabin.

       2.     No one is to go up  onto the cabin roof without the captain's permission.

       3.     No one is to climb up onto, stand up on, or move  around on the cabin roof while
              the ship is underway.

       4.     While underway, no one is to  move to  the aft deck by way of the outboard edge
              and handrail.

       5.     While underway, try to keep movement to a minimum.

       6.     When lifting equipment with the boom and winch, hard hats with face shields must
              be worn by anyone on the forward deck.

       7.     When collecting sediments, only the designated  collection crew are to  be on the
              forward deck.

       8.     When collecting sediments, personal protective equipment must be worn, including
              splash suit,  safety glasses, disposable gloves, outer green gloves taped to the suit,
              rubber boots or boot covers, and hard hats with face shields.

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                                                   SECTION: CHAPTER 9
                                                   VERSION: FINAL/MAY 1997
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9.      Full face shields are to be worn any time there is a strong possibility that sediment
       may be splashed upon you.   In  particular, face  shields should  be worn  when
       removing sediment  cores from the aluminum tube and \\hen washing sediment off
       equipment or the deck.

10.    No contaminated splash suits or boots are to be worn or carried into the cabin until
       decontaminated.

11.    When  sediment is  present on the forward deck,  no one is to step out onto the
       forward deck  unless, at a minimum,  a Tyvek® suit, disposable gloves,  boots and
       safety  glasses  are worn.  Once contaminated, this clothing is not to be worn back
       into the cabin  until  decontaminated.

12.    Heat-related emergencies  are one of our  primary  concerns during the summer.
       Many  times heat-related risks are a greater threat to our safety than the  possibility
       of  chemical contamination.   Good  judgment is  essential.   Pace yourself by
       knowing your  limits.  Field work is  not a race and above  all not a competition.
       During extreme heat, the Captain/Safety Officer will be monitoring your condition
       and recommending  you drink  plenty of water.  You may also  be requested  to
       remove your safety clothing and take a break in the sir conditioned cabin.  If you
       feel the effects of heat stress, inform the Captain/Safety Officer.

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APPENDICES
                             TABLE OF CONTENTS
APPENDIX A

APPENDIX B

APPENDIX C

APPENDIX D

APPENDIX E
APPENDIX F



APPENDIX G

APPENDIX H

APPENDIX I

APPENDIX J
Respiratory Protection Program

Medical Monitoring Programs

Hearing Conservation Program

Hazard Communication Program

Confined Space Entry Program

Attachment 1 - Confined Space Entry Program Checklist
Attachment 2 - Confined Space Entry Permit
Attachment 3 - References

Hot Work Procedures

Attachment 1 - Hot Work Permit

Lockout/Tagout Program

Bloodborne Pathogens Program

Personal Protective Equipment Program
Chemical Hygiene Plan
                   Attachment 1 -
                   Attachment 2 -
                   Attachment 3 -

                   Attachment 4 -
                   Attachment 5 -
                   Attachment 6 -

                   Attachment 7-
                  29 CFR 1910.1450, REVISED JULY 1, 1992:
                  Occupational Exposure to Hazardous Chemicals in
                  Laboratories
                  Storage for Chemical Compatibility
                  Standard Operating Procedures for Hazardous
                  Material Storage and Spill Prevention
                  Weekly Inspection Checklists
                  SOP for Hazardous and Common Waste Disposal
                  Radiation Safety SOP for R/V Lake Guardian
                  Laboratories
                  CRL Radiation Safety Manual
APPENDIX K
Heavy Equipment Practices For Sediment Sampling

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APPENDICES
                             TABLE OF CONTENTS
APPENDIX L


APPENDIX M

APPENDIX N

APPENDIX O


APPENDIX P

APPENDIX Q

APPENDIX R
APPENDIX S
APPENDIX T

APPENDIX U
Chemical Hygiene Standard Operating Procedures for US EPA GLNPO
Research Vessels

GLNPO Occupant Emergency Plan

R/V Mudpuppy Manual

Policy, Memorandums, Standard Operating Procedures, Regulatory
Updates

Welcome Aboard & Safety Orientation

Marine Sanitation Standard Operating Procedures

R/V Lake Guardian Hazardous Material Contingency Plan
                   Attachment 1
                   Attachment 2
                   Attachment 3
                   Attachment 4
                   Attachment 5
                   Attachment 6
                   Attachment 7

                   Attachment 8
                  Waste Stream Analysis
                  Laboratory Floor Plan
                  Chemical Storage by Room
                  Emergency Phone Numbers
                  Emergency Equipment Locations
                  Emergency Response Team
                  OSHA 29 CFR 1910.120 Health and Safety
                  Compliance Procedures
                  Notification to Emergency Assistance Authorities
Procedures for the Transportation of Hazardous Materials/Waste by
GLNPO Via Government Vehicle and Government Driver

Attachment 1       Bill of Lading
Attachment 2       DOT Form F 5800.1
Attachment 3       Sample Uniform Hazardous Waste Manifest

R/V Lake Guardian Environmental Compliance Manual

Non-Friable Asbestos Operations and Maintenance Plan

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APPENDICES
                               TABLE OF CONTENTS
APPENDIX V
ANNUAL AND PERIODIC INSPECTIONS AND SURVEYS
                    Attachment 1
                    Attachment 1.1
                    Attachment 1.2
                    Attachment 1.3
                    Attachment 1.4
                    Attachment 1.5
                    Attachment 1.6
                    Attachment 1.7
                    Attachment 1.8
                    Attachment 1.9
                    Attachment 1.10
                    Attachment 1.11
                    Attachment 2
                    Attachment 3
                    Attachment 4
                    Attachment 5
                    Attachment 6

                    Attachment 7
                    Attachment 8
                    Attachment 9
                    Fire Protection Systems SOPs
                    Fire Detection and Alarm System
                    Fire Alarm Checklist
                    Monthly Inspection Checklist for Fire Extinguishers
                    Weekly Inspection Checklist for Fire Extinguishers
                    Container Lab Extinguishing Systems
                    Galley Extinguishing System
                    Emergency Lighting
                    Hre Pumps, Main Hose, Hose Stations
                    Engine Room Carbon Dioxide Extinguishing System
                    R/V Lake Guardian Fire Alarm Checklist
                    Life Safety Equipment Drawing
                    Emergency Shower & Eyewash Inspection Form
                    Vessel Sanitation Standard Operating Procedures
                    Food Service  Inspection Form
                    Laboratory Hood Evaluation Sheet
                    Monthly Inspection Self-Contained Breathing
                    Apparatus
                    Hazardous Waste Storage Area Inspection Checklist
                    Spill Cart Checklist
                    GLNPO Specifications for Dry-Docking and
                    Overhaul Inspections

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                                                          SECTION:     APPENDIX A
                                                          VERSION:     FINAL/MAY 1997
                                                          PAGE 1 OF 23
APPENDIX A:  RESPIRATORY PROTECTION PROGRAM
Purpose

The purpose of this program is to provide written standard operating procedures governing the selection
and use of respirators as required by EPA Order 1440.3, Respiratory Protection and by the Occupational
Safety and Health Administration (OSHA) Regulations 29 CFR 1910.134; see Safety Office for copies.
Background

OSHA has set permissible exposure limits for many workplace airborne toxic materials.  If employee
exposure to the  toxic materials exceeds these limits, the regulations require that feasible  engineering
controls and/or administrative controls be installed/instituted. If engineering/administrative controls cannot
be implemented, the Agency is required to provide appropriate, approved respiratory protection for its
employees.  Respirators are the least acceptable means for reducing personnel exposures; they provide
good protection only if properly selected and fitted, worn by employees when needed, and replaced when
their service life is over.  Despite these difficulties, respiratory protective devices are the only means of
protection available  to employees when engineering and work practice controls are  not feasible or
inadequate.

EPA  Order  1440.3, Respiratory Protection, defines the Agency policy,  responsibilities, and basic
requirements for a respiratory protection program.  The purpose of the program is to ensure the safety of
employees whose jobs require the use of respiratory protective devices. EPA Management is required to
establish and implement a respiratory protection program for the Region and its employees, who engage
in activities in which atmospheres contain or are suspected of containing unhealthy quantities of airborne
contaminants, atmospheres that have insufficient oxygen content, or where there is the threat of imminent
release of toxic agents.  Respiratory protection may also be  necessary for routine but infrequent operations
and for non-routine  operations in which  the  employee is exposed briefly  to high concentrations of a
hazardous substance.
Selection of Respiratory Protection Devices

The proper selection of respiratory protective devices basically involves three steps:

        1.  Identification of the hazard.
        2.  Evaluation of the hazard.
        3.  Selection of the appropriate approved respiratory protective  device based on the first two
           considerations.

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                                                         SECTION:      APPENDIX A
                                                         VERSION:      FINAL/MAY 1997
                                                         PAGE 2 OF 23
Identification of the Hazard

To  identify respiratory hazards, it  is important to have working knowledge of the kinds of hazardous
atmospheres which require the use of respirators.   Contaminated atmospheres and  oxygen-deficient
atmospheres are the two types of atmospheres which present respiratory hazards.

Contaminated Atmospheres

Toxic materials can enter the body primarily in three ways:  (1) by ingestion, through the gastrointestinal
tract, (2) by absorption through the skin or through cuts and punctures, and (3) by inhalation through the
respiratory system. The respiratory system not only presents the quickest and most direct avenue of entry
into the body, but for many agents the lungs are also  the critical target.  Airborne contaminants include
solid and liquid particulate matter and gaseous material, whether a true gas or vapor, or a combination of
these.

Gaseous Contaminants: These contaminants are of two types:

        A. Gases are fluids which exist in a gaseous state at ordinary temperature and pressure, such as
           carbon dioxide.   Such substances are solids  or liquids only at much lower temperature or
           much higher  pressures than are commonly found in the work environment.  Carbon dioxide,
           is a gas at room temperature, but it occurs  as solid "dry ice" at  low temperature, or as a liquid
           in a pressurized tank.

        B. Vapors are a gaseous state of a  substance that is solid or liquid at ordinary temperature and
           pressure.  Vapors  are  formed by the evaporation of substances, for example, acetone or
           trichloroethylene, which ordinarily occur  as liquid.

Particulate Contaminants: Particulate contaminants are  suspended particles or droplets of a substance.
Many of these particles can remain suspended in air indefinitely and are easily inhaled:

There are three types of particulates:

        A. Dusts are solid particles produced by such  processes as grinding,  crushing, and mixing of
           powder compounds.

        B. Mists are tiny liquid droplets suspended  whenever liquid is  sprayed, vigorously mixed, or
           otherwise  agitated.

        C. Fumes are solid condensation particles  of small particle size.

Combination Contaminants:  The two basic forms of contaminated atmospheres, gaseous and particulate,
frequently occur together.

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                                                        SECTION:
                                                        VERSION:
                                                        PAGE 3 OF 23
                                    APPENDIX A
                                    FINAL/MAY 1997
Oxygen Deficient Atmospheres

In an oxygen deficient atmosphere, the problem is not the presence of something harmful, but the absence
of something essential.  These atmospheres are most commonly found in confined and poorly ventilated
spaces.  Oxygen deficient atmospheres are classified as either immediately dangerous to life or health
(IDLH) or not immediately dangerous to  life or health depending on the  oxygen concentration in the
atmosphere. (An oxygen deficient atmosphere that contains less than 16 volume percent of oxygen in the
atmosphere at sea level is IDLH. An oxygen deficient atmosphere not immediately dangerous to life and
health is an atmosphere having an oxygen concentration between  16 and 19.5 volume percent of oxygen
in the atmosphere at sea level.  Normal oxygen content is 20.9 volume percent in the atmosphere.)

Oxygen deficient atmospheres occur in two different ways: (1) Oxygen may be "used up" by a chemical
reaction, and (2) Oxygen  is  displaced  by another gas.   There is  no  definition of oxygen deficient
atmosphere that has been universally accepted.  OSHA has adopted and EPA accepts an oxygen deficient
atmosphere as one that contains less than 19.5 volume percent of oxygen in the atmosphere at sea level.

Effects of Oxygen Deficiency
O: Vol % at Sea Level
16-12
Physiological Effect

Increased breathing volume.
Accelerated heartbeat.
O2 Vol % at Sea Level
14-12
O2 Vol %at Sea Level
14-10
10-6
Less than 6
Physiological Effect

Impaired attention and thinking.
Impaired coordination.

Physiological Effect

Very faulty judgment.
Very poor muscular coordination.
Muscular  exertion causes rapid fatigue  that  may  result in
permanent heart damage.
Intermittent respiration.

Nausea.
Vomiting.
Inability to perform rigorous movement, or loss of all movement.
Unconsciousness, followed by death.

Spasmatic breathing.
Convulsive movements.
Death in minutes.

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                                                           SECTION:      APPENDIX A
                                                           VERSION:      FINAL/MAY 1997
                                                           PAGE 4 OF 23
Evaluation of the Hazard

Respiratory protective devices, according to Agency policy and OSHA regulations shall be required in the
following types of situations:

        1.  When there is a  high potential for sudden release of toxic airborne substances or there has
           been such a release.
        2.  When making entries into environments or locations where there is reasonable belief that a
           hazardous environment is present; for example, entering hazardous waste or  spill sites and
           manholes.
        3.  During infrequent, but routine  operations, where engineering controls are not feasible or
           adequate  for the  toxicity of the material involved.

It is important to assess the potential hazards and the degree of control that can be exercised over each
situation.   Responsibility resides with the project  leader/inspector.   The respiratory protective  device
selected in each  situation  will depend on the information  from the  qualitative  and quantitative
determination of the hazard.

The person who evaluates the respiratory  hazard must have the cooperation of others in  obtaining
information on the work area, work activities and other materials to properly evaluate and determine the
appropriate respiratory protective device that will provide the best  protection.  Consideration of these
questions will help in the selection of the correct equipment:

        1.  Does the atmosphere oxygen level meet with  standards? Is the  atmosphere oxygen level
           expected  to remain constant or decrease?
        2.  What is the contaminant?  Is it a gas, vapor, mist, dust, or fume?
        3.  What is the estimated concentration of the contaminant?  Have measurements been taken?
        4.  Could the contaminant be considered immediately dangerous to life or health?
        5.  Is the contaminant flammable?  Does the concentration approach the lower explosive limit?
           Do dust concentrations create a potential explosion problem?
        6.  Does the  contaminant have adequate warning properties, e.g., smell., irritation?
        7.  Will the  contaminant irritate the eyes at  estimated concentration? Is eye protection also
           needed?
        8.  What type(s) of respirators will  provide the required degree of employee protection?9.Is the
           recognized contaminant the only contaminant present?
        10. If the contaminant is a gas or vapor,  is there an effective sorbet for the respirator canister?
        11. Can the contaminant be absorbed through the skin? If it can, will it result in a serious injury?
        12. Does the facility require use of respiratory protection?  Is there a facility/site  specific safety
           plan available?  Does it specify respiratory protection requirements?
        13. Is there any  onsite screening or test data available?
        14. Are OSHA respiratory protection requirements applicable to the facility, site or activity?

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Selection of Approved Respiratory Protective Devices

The selection of approved respiratory protective devices shall be based on these considerations:

        1.   The nature of the hazardous operation or process.
        2.   The type of respiratory hazard.
        3.   The location of the hazardous area in relation to the nearest area having respirable air.
        4.   The period of time the respiratory protection will be needed.
        5.   The employee's activities in the hazardous area.
        6.   The physical characteristics, functional capabilities, protection factors and limitations of the
            respiratory protection devices.

The NIOSH approval on a respirator has the following information:

        •   An assigned identification number placed on each unit.
        •   A label identifying the type of hazard  for which the respirator is approved.
        •   Additional information on the label which give limitations and identifies the component parts
            for use with the basic  unit.

Attachment A to EPA Order  1440.3 (See safety office for copy) provides a "Decision Logic Table for
Respiratory Protective Device Selection."   These are general  guidelines.   Written standard operating
procedures  governing the selection and  use of respiratory protective  devices shall be  established for
specific  situations, where  necessary.  These  may  be in  the  form of facility/site specific  plans  or
program-specific  safety plans, such as for NESHAP-Asbestos inspections (Appendix  C of EPA Order
1440.3). Types of respiratory protective  devices are summarized  in Appendix D of EPA Order 1440.3.

SELECTION

In general ANSI Z88.2-1980 states that the selection of the proper approved respirator depends upon:

        •  The nature of the hazard.

        •  The characteristics of the hazardous operation or process.

        •  The location of the hazardous area with respect to a safe area having respirable air.

        •  The period of time for  which respiratory  protection may be needed.

        •  The activity of workers in the hazardous area.

        •  The physical characteristics, functional  capabilities, and limitations of  respirators of various
           types.

        •  The respirator/protection factors and respirator fit.

All these criteria must be considered in the selection of a respirator.  The Joint NIOSH/OSHA Standards

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Completion Respirator committee devised a "Respirator Decision Logic" based on the above criteria.

ANSI Z88.2-1980 also describes the suitability of a particular respiratory protective device for oxygen
deficient or Immediately Dangerous to Life or Health (IDLH) atmosphere. OSHA regulations require the
use of approved respirators. Respirators are tested at the NIOSH Testing Laboratory in Morgantown, West
Virginia in accordance with the requirements of 30 CFR Part  11 and are jointly approved by the Mine
Safety and Health Administration (MSHA).

AIR-PURIFYING RESPIRATORS

I.    Introduction

     Respiratory protection must be used when the concentration of a substance in the ambient atmosphere
     exceeds a personal exposure limit. Several exposure limits  used to determine the need for respiratory
     protection. In order of precedence, these are the OSHA Permissible Exposure Limits (PELs), NIOSH
     Recommended Exposure Limits (RELs), and the ACGIH Threshold Limit Values (TLVs).  If none
     of  these are available, other published data may be used.

     Air-purifying respirators may be used only if all of the following requirements are met:

        •   The identity and concentration of the contaminant are known.

        •   The ambient concentration of a contaminant  is below the Immediately  Dangerous to Life
            or Health (IDLH) concentration.

        •   The oxygen content in the atmosphere is greater than  19.5%.

        •   The respirator assembly is approved for protection against the specific  concentration of a
            contaminant.

        •   There is periodic monitoring of the work area.

        •   The respirator assembly has been successfully fit tested on the user.
II.   Respirator Construction

     An air-purifying  respirator (APR) consists of a facepiece and a purifying element.  In some APR
     designs there are combined in a single unit, more often they are separate components.  There are
     several basic designs of air-purifying respirators.

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         •   Disposable Dust Respirators

            These are generally constructed using cloth or paper as the filter element. A satisfactory face
            to respirator seal is difficult to achieve with this type of respirator. Most respirators of this
            design are not approved.  Those which are approved are used for protection against dusts and
            mists. Some may be approved for asbestos use.

         •   Emergency Escape Mouthbit Respirators
            Mouthbit respirators  are approved for escape use only. The mouthpiece  containing the
            cartridge element is held in place by the teeth and a clamp is  used to seal the nostrils.

         •   Quarter-Mask Respirators (Type B Half-Mask)
            The quarter-mask  respirator is  used  for dusts (airborne exposure limits not less than 0.05
            mg/m3).  The  facepiece fits from the top of the nose to the top of the chin.

            Half-Mask Respirators (Type A Half-Mask)
            The half-mask respirator has approved cartridges for pesticides, organic  vapors, dusts, mists,
            fumes, and several other combinations.  A half-mask  respirator fits from above the nose to
            under the chin.

         •   Full-Face Respirators
            A full-face respirator  provides full face protection, including the eyes.  It has a much higher
            Assigned Protection Factor than either a quarter or half-mask respirator. The full-face mask
            may be used with  a wide  variety of  filtration media.  This type of respirator may be  used
            with  cheek-mounted  cartridges,  chin-mounted  cartridges,  chin-mounted  canisters,  or
            chest/back-mounted canisters.

         •   Powered Air-Purifying Respirators
            Powered air-purifying respirators utilize pumps or fans to  force air through the purifying
            elements. This eliminates breathing  resistance and may help to  maintain positive pressure
            in the facepiece.  Powered air-purifying respirators are available in quarter, half, and full-face
            designs as well as with specialized helmet assemblies.

III.  Facepiece

     The facepiece is the means of sealing  the respirator assembly to the user's face.   The facepiece
     consists of the lens  (full  face  only), mask suspension,  and a means of attaching the  filtration
     elements.  Inhalation check valves are used to prevent moist exhaled air from being passed through
     the  filter element.  Exhalation check valves are used to allow exhalation through the diaphragm.
     Each respirator manufacturer utilized different means for  attaching components to  the facepiece.
     This prevents mixing parts  from different  manufacturers (hybridization) which  would void  a
     respirator's approval.

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     Although many configurations exist, only four types of facepiece-element assemblies are permissible
     for use with hazardous materials:

        •   Half-mask with twin cartridges or filters.

        •   Full-face mask with twin cartridges or filters

            Full-face mask with chin-mounted canister

        •   Full-face mask with harness-mounted canister (gas mask)

     The full-facepiece is the recommended facepiece for use.  It provides eye protection, is easier to fit,
     and has an Assigned Protection Factor greater than the half-mask.


IV.  Air-Purifying Elements

     Respiratory hazards can be broken down into two classes: particulates and vapors/gases. Particulates
     are filtered by mechanical  means, while vapors and gases are removed by  sorbents that  react
     chemically with them. Respirators with the appropriate cartridge can filter or remove both classes
     of hazards.

     A. Particulate-Removing Filters

        Particulates can occur as dusts, fumes, or mists. The  particle size can range from macroscopic
        to microscopic, and their toxicological effects can be severe or innocuous. The hazard posed by
        a particulate can be determined by its exposure limit (EL).  A nuisance particulate will have an
        EL of 10 mg/m3, while a toxic particulate may have an EL well below 0.05 mg/m3.

        Mechanical filters are classified according to the protection for which  they are approved under
        schedule 21C of 30 CFR Part 11. Most particulate filters are approved  only for dusts and/or
        mists with Els equal  to  or greater than 0.05  mg/m3.  These dusts are usually considered to
        produce  pneumoconiosis and  fibrosis.   Such  filters have an  efficiency of 80-90% for 0.6
        micrometer particles.

        Respirators approved for fumes  are more efficient, removing 90-99% for 0.6 micrometer
        particles.  This type of respirator  is approved  for dusts, fumes and mists with ELs equal  to or
        greater than 0.05  mg/m3.

        Finally there is a high efficiency  filter which is 99.97% effective against particles 0.3 microns
        in diameter.  It is approved for dusts, mists, and fumes  with an EL  less than 0.05 mg/m3.

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    Mechanical filters  load with  participates as they  are used.  As they do, they become more
    efficient, but also become more difficult to breathe through.  When a mechanical filter becomes
    difficult to breathe through it should be replaced.

B.  Gas and Vapor-Removing Cartridges and Canisters

    When selecting a gas or  vapor-removing element, it must be chosen for protection against a
    specific type of contaminant. Some of the commonly employed types of chemical cartridges and
    canisters and their OSHA-required color coding are listed in Table 2-1. This table has been
    excerpted from the OSHA respirator regulations for general industry (29 CFR 1910.134).

    1.    Style and Size

         Gas and vapor-elements are available in different styles. The physical differences are: (1)
         size and (2) means  of attachment to the facepiece.  The smallest elements are cartridges
         which contain 50-200 cm3 of sorbet and attach directly to the facepiece, usually in pairs.
         Chin canisters have a volume of 250-500 cm3  and  are attached to a full-facepiece.  Gas
         mask, or industrial-size canisters contain 100-2000  cm3 and are attached by a harness to
         the wearer's front or back and connected to the full-facepiece by a breathing hose.

         The difference  in applications is the Maximum Use Concentration (MUC) for which the
         cartridge or canister can be used in accordance with its NIOSH/MSHS approval.  For
         example, organic vapors can be removed by the appropriate  cartridges, chin canister, or
         gas-mask canister.  Cartridges are approved for use in atmospheres up to 1000 ppm (0.1%)
         organic vapors, chin style canisters up to 5000 ppm (0.5%), and gas mask canisters up to
         20,000  ppm  (2.0%).   However,  no air-purifying  respirator is permitted  in an IDLH
         atmosphere.

    2.    Service Life

         Each sorbet has a finite capacity for removing contaminants and when this limit is reached
         the cartridge or canister is said to be saturated.  At this point the element will allow the
         contaminant to pass through and enter the facepiece.  The length  of time a cartridge or
         canister will effectively sorb the contaminant is known as the service life of the element.
         Service life of a type of cartridge or canister is dependent on several factors:  the breathing
         rate of the wearer; contaminant concentration; and sorption efficiency.

         a.  Breathing Rate

         If the breathing rate of the user  is rapid, the  flow rate of the contaminated air drawn
         through the cartridge is greater than it  is at a moderate or slow respiration rate. A higher
         flow rate brings a larger amount of contaminant in  contact with the  sorbet in a  given
         period of time which, in turn, increases the rate of  sorbet saturation and shortens service
         life.

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      b.  Contaminant Concentration

      The expected service life of an organic vapor cartridge decreases as ambient contaminant
      concentration increases. As concentration goes up, the mass flow rate increases, bringing
      more contaminant in contact with the sorbet in a given period of time.  For example, at
      any constant breathing rate, ten times as much contaminant contacts the element when the
      concentration is 500 ppm compared to 50 ppm.

      c.  Cartridge Efficiency

      Chemical sorbents  vary in their ability to remove  contaminants from  air.   Table 2-2
      compares the efficiency of organic vapor cartridges for a number of solvents by recording
      the  amount  of time  until  a  1%  breakthrough  concentration  was  measured  in  the
      cartridge-filtered air.  The initial test concentration is 1000 ppm of solvent vapor;  the
      breakthrough concentration is 10 ppm.  From the table it can be seen that it takes 107
      minutes for chlorobenzene to reach a 1% breakthrough, while it only takes 3.8 minutes for
      vinyl chloride.  The sorbet (activated carbon) in the organic vapor cartridge is much better
      for  removing chlorobenzene than  vinyl chloride  under the test conditions.  Cartridge
      efficiencies need to be considered when selecting and using APRs.

3.     Warning Properties

      A warning property is used as a sign that a cartridge or canister in use is beginning to lose
      its effectiveness.  A warning property can be detected as an odor, taste, or irritation.  At
      the  first such signal, the old cartridge or canister must be  exchanged for a fresh one.
      Without a warning property, respirator efficiency may drop without the knowledge of the
      wearer, ultimately causing a health hazard.

      Most substances have  warning properties  at some concentration.  A  warning property
      detected only at dangerous levels, that is, greater than the EL, is not considered adequate.
      An odor, taste, or irritation detected at extremely low concentrations is also not adequate
      because the warning is being given all the time or long before the filter begins to lose its
      effectiveness. In this case, the wearer would never realize when the filter actually becomes
      ineffective.

      The best concentration for a warning property is to be first detected is around the EL.  For
      example, toluene has an odor threshold of 40 ppm and an EL of 100 ppm. This is usually
      considered an adequate warning property.  Conversely, dimethylformamide has an EL of
      10 ppm and  an odor threshold of 100 ppm.  An odor threshold  ten times the EL is not an
      adequate warning property.

      If a substance causes  rapid olfactory  fatigue (that  is, the sense of smell  is no  longer
      effective), its odor is not an adequate warning property.  For example, upon entering an
      atmosphere containing hydrogen sulfide, the odor is quite noticeable. After a short period
      of time, it is no longer detectable.

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Use of Respiratory Protective Devices

Selecting the respirator appropriate for a given hazard is important,  but equally important is using the
selected device properly. Proper use can be ensured by training users in selection, use, and maintenance
of respiratory devices.

TRAINING AND FIT TESTING

Training

EPA Order 1440.3 requires that employees receive a minimum of six hours training initially, and two to
four hours annually, thereafter, in respiratory protection. Training will be provided initially as part of
Region V's "24 Hour Health and Safety Training for Field Personnel" course, and subsequent training will
be provided as part of Region V's required  annual "8 Hour Refresher Training" course, and recorded via
Region V's normal safety record keeping and tracking system.

Fit Testing

The proper fitting of respiratory protective  devices requires the use of some type of fit test. The fit test
is needed to determine the proper match between the facepiece of
the respirator and the  face of the user. Two types of tests are necessary, qualitative tests and quantitative
tests.

     1.  Qualitative Tests: Fast, requiring no complicated expensive equipment, and are easily performed.
        Qualitative fit tests may be used, but are not substitute for annual quantitative fit tests which are
        required for  all respirator users. There are five types of qualitative tests:

        A.    Isoamyl acetate, a  low toxicity  substance with a banana oil like  odor, is used widely in
              testing the facepiece fit  of organic  vapor cartridge/canister respirators.  The substance is
              applied to a cotton wad inside an enclosure.  The enclosure can  be put together by the use
              of a plastic bag,  several hangers, and some cotton.  The user should put on the respiratory
              protective device in some area away  from the test enclosure  so that there is no  prior
              contamination of the cartridge or "pre-exposure" to the isoamyl acetate.

              The user should perform the following steps in order:

                  Normal breathing.

                  Deep breathing, as during heavy exertion.  This should not be done long enough to
                  cause hyperventilation.

              •   Side-to-side  and up-and-down  head movements.  These movements should not be
                  exaggerated, but should  approximate those that take place on the job.

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         •   Talking. This is most easily accomplished by reading a prepared text loudly enough
             to be understood by someone standing nearby.

         •   Other exercises may be added depending upon the need.

         The major drawback of the isoamyl test is that the odor threshold varies widely among
         individuals.  Also, the sense of smell is easily dulled  and may deteriorate during the test
         so that the user can  detect only high vapor concentrations. Another disadvantage is that
         isoamyl acetate smells pleasant, even in high concentrations; therefore, a user may say that
         the respirator fits although it has a leak.   A user may say that a respirator fits because
         he/she likes  the fit of a particular  inspirator or  is following the respirator selection  of
         another employee.  Conversely, a user may claim that a particular respirator leaks if it is
         uncomfortable, etc.  Therefore, unless the  employee is highly motivated toward wearing
         respirators, the results of the test must sometimes be suspect.

B.  Irritant Smoke Test - The irritant smoke test, similar to the isoamyl  acetate test in concept, is
    used widely in testing the facepiece  fit of high efficiency particulate filter respirators. This test
    can be used for both air-purifying and atmosphere supplying respirators, but an  air purifying
    respirator must have a high-efficiency filter. The test substance is an irritant (stannic chloride
    or titanium tetrachloride) which is available commercially in sealed glass tubes.  When the tube
    ends are broken and air passes through them, a  dense  irritating smoke is emitted.  If the user
    detects any of the irritant smoke, it means a defective fit, and adjustment or replacement of the
    respirator  is required.  The irritant  smoke test  must be performed with caution because the
    aerosol is highly irritating to the eyes, skin, and  mucous membrane. As a qualitative means  of
    determining respirator fit, this test has a distinct advantage in that the wearer usually reacts
    involuntarily to leakage by coughing or sneezing. The likelihood of giving a false indication  of
    proper fit is reduced.  Irritant  smoke tests are ineffective for positive pressure respirators
    operating in the demand and continuous mode.

C.  Taste Test - Utilizing sodium  saccharin and placing a hood over  the wearers head  is an
    acceptable method for Atmosphere Supplying Respirators. (Normally for single use respirators.)

D.  Negative Pressure  Test - This test (and the positive pressure test) should be used only as a very
    gross determination of fit.  The  wearer should use this test just before entering the hazardous
    atmosphere. In this  test, the user closes off the inlet of the canister, cartridge(s) or filter(s) by
    covering with  the  palm(s) or squeezing the breathing tube so that it does not pass air;  inhales
    gently  so that  the facepiece collapses slightly;  and holds his/her breath for about ten seconds.
    If the facepiece remains slightly collapsed and no inward leakage is detected, the respirator has
    been properly donned and the exhalation valve and facepiece are not leaking.   This  test,  of
    course, can only be used on respirators with tight-fitting facepieces.  Although this test is simple,
    it has  severe drawbacks;   primarily that the wearer must  handle the respirator   after it has
    supposedly been positioned on his/her face. This handling can modify the  facepiece seal.  A
    second drawback  is  that with a negative pressure  in a facepiece, a leaking facepiece  may  be
    drawn  tightly to the  face to form a good seal, giving a false reading of a good seal.

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     E.  Positive Pressure Test - This test, similar to the negative pressure test, is conducted by closing
        if the   exhalation valve or breathing tube and exhaling gently into the facepiece.  The fit is
        considered satisfactory if slight positive pressure can be built up inside the facepiece without any
        evidence of outward leakage. For some respirators, this method requires that the wearer remove
        the exhalation valve cover;  this often disturbs the fit of the respirator to the wearer.  Therefore,
        this test should be used sparingly if it requires removing and replacing a valve cover.  The test
        is easy for respirators whose valve cover has a single small port that can be closed by the palm
        or a finger.

    Quantitative Tests - Quantitative respirator performance tests involve placing the user wearing the
    device in an atmosphere  containing an easily detectable, relatively non-toxic gas, vapor or aerosol.
    The atmosphere inside the respirator is sampled continuously through a probe in the respiratory inlet
    covering.  The leakage is expressed as  a percentage of the test atmosphere  outside the respirator,
    called "percentage of penetration," or simply "penetration."  The greatest advantage of a quantitative
    test is that it indicates respirator fit numerically, and does not rely on a subjective response.

    EPA Employees are required to receive an annual quantitative fit test as arranged by  the Regional
    Safety Officer.  The irritant smoke qualitative test will be used as an interim measure.  (This method
    is less subjective than the isoamyl acetate method.)  Both the negative or positive pressure test will
    be used by the employee when donning  a respirator  to check the seal.  Blue certification cards must
    be signed by the individual performing the  fit test.
MEDICAL MONITORING

As part of the annual medical monitoring examination, employees performing tasks requiring the use of
respiratory protective devices must be certified physically capable of performing the work while wearing
the  devices.  A  blue certification card will be issued to employees by a physician certifying that the
employee is physically able to wear a respirator. The certification card must be available at the time of
the  respirator fit test and signed by the fit test operator.
INVENTORY AND PERSONAL ISSUANCE

Inventory

The Regional Safety Manager shall maintain a general inventory of respiratory equipment and supplies
in his/her  custody, and  administer the regional  budget for equipment  purchases, for non-CERCLA
activities. Part of the Regional Safety Manager's budget is administering funds for all personal protective
equipment including purchase for all respiratory equipment and respiratory equipment supplies.

When additional respirators or respiratory equipment are needed, those needs should be transmitted to the
Regional Safety Manager as soon as possible and  the procurement should be coordinated with him/her.

Additionally, in each Branch where employees use respiratory protection devices, a general inventory of

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respiratory equipment and supplies  may be  kept  by the Branch Safety Officer or other appropriate
individuals, but if kept shall be the  responsibility of the Branch Supervisor.  This inventory shall be
available to the Regional Safety Manager for  his/her annual inspection.

Personal Issuance

Field and laboratory personnel subject to contaminated atmospheres (as previously defined) will be issued
an appropriate respirator with an initial supply of filters.  Other equipment may be personally issued as
needed.   The  immediate supervisor or his/her designee, is  responsible for issuing equipment and
maintaining records of the issuances.  Respirators should be the full  face type whenever possible.

SPECIAL CONSIDERATIONS IN RESPIRATOR USE

        1.      Respiratory protective devices shall not be worn when any condition prevents a good seal.
               Specific conditions not permitted are as follows:

               A.     Any facial hair lying between the sealing surface of a respirator facepiece and the
                      wearer's skin that will prevent a good  seal shall not be allowed.  This includes
                      stubble, a moustache, sideburns or a beard that extends outward between the face
                      and the sealing surface of the respirator.

               B.     Spectacle temple bars or  straps  that  pass  between the  sealing surface of a
                      facepiece and the wearer's  face prevent a good seal and,  therefore, shall not be
                      permitted with a full-face respiratory protective device.  Individualized eye glasses
                      mounted to the facepiece will  be furnished.

        2.      Employees with perforated  eardrums shall not wear respirators.

        3.      Contact lenses shall not be permitted while wearing a respirator.

        4.      Eating, drinking, smoking, chewing gum and applying cosmetics shall not be permitted
               while wearing a respirator.
Inspection, Maintenance, Repair, and Storage of Respiratory Protective Devices

Proper inspection, maintenance, storage, and repair of respiratory devices are mandatory to insure that
these devices protect the health and safety of the employees when in use.
Inspection

An important part of respirator maintenance program  is the inspection of the devices.  If performed
properly, inspections will identify damage or malfunctioning respirators and help to maintain their state
of readiness. All respiratory protective devices must be inspected monthly or before each use. A record

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must be kept of inspection dates and findings. Responsibility for inspection and record keeping resides
with the supervisor (who may delegate the responsibility to the Branch Safety Officer), for equipment in
general inventory and the employee for personally issued equipment.  Inspection guidelines for various
respiratory protection devices are provided in Appendix E of EPA Order  1440.3.
Maintenance

All respiratory protective devices shall be cleaned and disinfected after each use by the user or by another
designated person.

The actual cleaning may be accomplished in a variety of ways:

        1.      The respiratory protective device should be washed with detergent in warm water using
               a brush , thoroughly rinsed in clean water, and then air dried in a clean place.  Care
               should be taken to prevent damage from rough handling.  This method is an accepted
               procedure for a small group or unit of employees where each employee cleans his/her own
               respirator.

        2.      A standard domestic type dish or clothes washer may be used if a rack is installed to hold
               the facepieces in a fixed position. (If the facepieces are placed loose in the washer, they
               may be damaged.)   This method  is especially useful in a large unit or group where
               respirator usage is extensive.  *Handy-wipe disinfectants may be used as a substitute for
               the above methods.

Detergents and disinfectants:   If possible, detergents containing a  bactericide  should be used.   Organic
solvents should not be used, as they can deteriorate the rubber facepiece.  If the above combination is not
available, a detergent may be  used, followed by a disinfecting rinse.  Reliable disinfectants may be made
from some available household solutions.

        1.      Hypochlorite   solution  [50  parts  per  million (ppm) of chlorine]  made  by adding
               approximately two tablespoons of chlorine bleach per gallon  of water.  A two-minute
               immersion  is  sufficient to disinfect the respirator.

        2.      Aqueous solution of iodine (0.8ml tincture of iodine in one liter.  The  iodine 7%
               ammonium and potassium iodine, 45% alcohol and  48% water.)  Again,  a two-minute
               immersion  is sufficient  and will not  damage the rubber and plastic in the respirator
               facepieces.  Check with  the manufacturer to find out the proper temperature for the
               solutions.

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If the respirators are washed by hand, a separate disinfecting rinse may be provided.  If a dish or clothes
washing machine is used, the disinfectant must be added to the rinse cycle, and the amount of water in
the machine at the time will have to be
measured to determine the correct amount of disinfectant to  be added.

Rinsing:  The cleaned and disinfected  respirators should be rinsed thoroughly in clean water (120° F
maximum) to remove all traces of detergent, cleaner and sanitizer, and disinfectant. This is very important
to prevent dermatitis.

Drying: The respirators may be allowed to dry by themselves on a clean surface. They may also be hung
from a horizontal wire, like drying clothes, but care must be taken not to damage the facepieces.
Continued use of respiratory protective devices may require periodic repair or replacement of component
parts of the equipment.  Such repairs and parts replacement must be done by a qualified individual(s).
Equipment in need of repair will be given to the Supervisor or Safety Officer designee, who will either
arrange for the repair of the equipment or its disposal if it is not repairable.

Replacement of parts and repair of air-purifying respirators, in most cases, present little problem. Most
equipment manufacturers supply literature which details the component parts of their respirator and include
servicing information.   The manufacturer will also provide replacement parts.  Replacement parts for
respiratory  protective devices  must be those of the manufacturer of the equipment. Substitution of parts
from a different brand or type of respirator  will invalidate the approval of the respirator.

(All respirators and parts shall be NIOSH or MSHA approved with the appropriate TC  number.)

Defective air-supplying respiratory equipment, with the exception of the SCBA, can be repaired and worn
if broken parts are replaced by a qualified individual, again with the aid of the manufacturer's literature
and parts. Maintenance of SCBA equipment is more difficult, primarily because of the valve and regulator
assembly.  Because of this, regulations require that SCBA equipment be returned to the  manufacturer or
certified repair person for adjustment or repair.
Care  that has  been taken to repair and  maintain  respirators can  be  negated by improper storage.
Respiratory protective equipment must be stored to protect it from  dust,  sunlight, heat, extreme  cold,
excessive moisture, and damaging chemicals. Leaving a respirator unprotected can lead to damage of the
working parts or permanent distortion of the facepiece,  thus making  it ineffective.

-------
                                                         SECTION:      APPENDIX A
                                                         VERSION:      FINAL/MAY 1997
                                                         PAGE 17 OF 23
After cleaning and disinfecting the respirators, they should be placed individually in plastic bags capable
of being sealed until reissue.  They should be stored in a single layer with the facepiece and exhalation
valve in a normal position to prevent the rubber or plastic from assuming a permanently distorted shape.

Equipment in general inventory will be stored in designated Iqckers or chests provided with the equipment.
 Personally issued equipment when not in  use will be stored in a personally issued equipment locker. In
the field, the equipment will  be transported in the  chests provided with the equipment or in a special
carrying bag.

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                                                          SECTION:     APPENDIX A
                                                          VERSION:     FINAL/MAY 1997
                                                          PAGE 18 OF 23
ATTACHMENTS

ADMINISTRATIVE RESPIRATORY PROTECTION PROGRAM
(To be Distributed to Employees When Issued Respirators)

General

The OSHA General Industry standard for respiratory protection, 29 CFR 1910.134, requires that a written
respiratory protection program be established by an employer. The following procedures are based on the
requirements for a minimum respirator program as established by the Occupational Health  and Safety
Administration.

OSHA's Requirements for a Minimal Respirator Program:

1.      "Written standard operating procedures  (S.O.P.) governing the selection and use of respirators
        shall be established."

2.      "Respirators shall be selected on the basis of hazards to which the worker is exposed."

3.      "The user shall be instructed and trained in the proper use of respirators and their limitations."

4.      "Where practicable, the respirators should be  assigned to  individual workers for their exclusive
        use."

5.      "Respirators shall be regularly cleaned and disinfected.  Those used by more than one worker shall
        be thoroughly cleaned and disinfected after each use."

6.      "Respirators shall be stored in convenient, clean, and sanitary location."

7.      "Respirators used routinely shall be inspected during cleaning.  Worn or deteriorated parts shall
        be replaced. Respirators  for emergency use,  such as self-contained breathing devices, shall be
        thoroughly  inspected at least once a month and after each use."

8.      "Persons should not be assigned to tasks requiring use of respirators unless it has been determined
        that they are physically able to perform the work and use the equipment.  The  local physician
        shall determine what health and physical conditions  are pertinent.  The respirator user's medical
        status should be reviewed annually."

9.      "Approved  or accepted respirators shall always be used."

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                                                          SECTION:      APPENDIX A
                                                          VERSION:      FINAL/MAY 1997
                                                          PAGE 19 OF 23
Guidelines

1.       The guidelines in this program are designed to help reduce employee exposures to occupational
        dust, fumes, mists, radionuclides, gases, and vapors.

2.       The primary objective is to prevent excessive exposure to these contaminants.

3.       Where feasible, exposure to contaminants will be eliminated by engineering controls (example,
        general and local ventilation, enclosure or isolation, and substitution of a less hazardous process
        or material.)

4.       When effective engineering controls are not feasible,  use of personal respiratory  protective
        equipment may be required to achieve the goal.
Responsibilities

1.      Management

       It is management's responsibility to determine what specific situations require use of respiratory
       equipment.  Management must also provide proper respiratory equipment to meet the needs of
       each specific situation.  Employees must be provided with adequate training and instruction  on
       all equipment.

2.      Management/Supervisory

       Superintendents, supervisors, foremen, or group leaders of each area are responsible for ensuring
       that all personnel under their control are completely knowledgeable of the respiratory protection
       requirements for the areas in which they work.  They are also responsible for insuring that their
       subordinates comply with all facets of this respiratory program,  including respirator inspection and
       maintenance.

3.      Employees

       It is the  responsibility of the employee  to  have an awareness of  the  respiratory protection
       requirements for their work areas (as explained by management.) Employees are also responsible
       for  wearing the  appropriate respiratory equipment according to proper instructions and  for
       maintaining the equipment in a clean and operable condition.

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                                                        SECTION:     APPENDIX A
                                                        VERSION:     FINAL/MAY 1997
                                                        PAGE 20 OF 23
ADMINISTRATION
1.      Name:	  Signature:	
       Regional Health & Safety Manager
       is responsible for overall program administration.

2.      Name:	  Signature:	
       Physician, Occupational Health Nurse
       is responsible for monitoring the health of company employees via a comprehensive medical and
       health program, including physical examinations.

3.      Name:	  Signature:	
       Either Regional Health & Safety Manager or Supervisor
       is responsible for selection, issuance, training, and fit testing of all respirators used in this Agency
       including recordkeeping  of the "Respirator Issuance and Training" card and "Job Description -
       Respirator Specification" form.
RECOMMENDED RESPIRATORY PROTECTION PROGRAM

Work Area Monitoring

Although it is not specifically discussed in the OSHA Requirements for a Minimal Respirator Program,
to assure the adequacy of a respiratory protection program, monitoring should be conducted on a periodic
basis to provide a continuing healthful  environment for employees.  Personal sampling equipment may
be used in accordance with accepted industrial hygiene standards to sample each  work area.  Results of
these samples will pinpoint areas where respiratory protection is required. A "Job Description - Respirator
Specification" Form will  also document what type of equipment  should be worn for specific hazards
present.


EMPLOYEE MEDICAL  MONITORING

1.      Pre-employment physical examinations are conducted on all employees  to assure that they are
        physically able to perform their work and can use respiratory equipment  as required.

2,      Yearly physical  examinations  will be  given  to regular employees  in order to assist them in
        maintaining their  health.  (At option of employer/physician or where  such exams are required by
        Local, State, and/or Federal Regulations).

3.      Biological monitoring in the form of blood and/or urinary analysis will be conducted on a yearly
        basis for all employees if appropriate (Employer/Physician determination).

4.      Records  must be  kept on file for the duration of employment and for 30  years following.

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                                                         SECTION:     APPENDIX A
                                                         VERSION:     FINAL/MAY 1997
                                                         PAGE 21  OF 23
RESPIRATOR INSPECTION AND MAINTENANCE

The following points should be considered for respirator inspection and maintenance:

1.     The wearer of the respirator should inspect it daily whenever it is in use.

2.     Respirators not discarded after one shift use, will be cleaned on a daily basis, according to the
       manufacturer's  instructions, by the assigned employee or other person designated by the respirator
       program coordinator.

3.     Respirators not  discarded after one shift use, will be stored in a suitable container away from areas
       of contamination.

4.     Whenever feasible, respirators not discarded after one shift use, will be marked or stored in such
       a manner to assure that they are worn only by the assigned employee.  If use by more than one
       employee is required, the respirator must be cleaned between uses.


EMERGENCY RESPIRATORY EQUIPMENT

Self contained breathing apparatus may be required in specific areas for emergency use. This equipment
will be used only by trained personnel when  it is  necessary to enter hazardous atmospheres.  The
following points should be considered:

1.     All potential users will be  fully trained in the use of this equipment.

2.     When the equipment is used, it will be tested in an uncontaminated atmosphere prior to entering
       the hazardous area if possible.

3.     An employee will not work with this apparatus in a hazardous atmosphere on an individual basis.
       At least one additional employee suitably equipped with a similar breathing apparatus must be in
       contact with the first employee and must be available to  render assistance if necessary.

4.     This equipment will be inspected monthly by trained department or group personnel.  Inspection
       and maintenance information will  be maintained by the Branch Supervisor Safety Officer.

-------
                                                   SECTION:     APPENDIX A
                                                   VERSION:     FINAL/MAY 1997
                                                   PAGE 22 OF 23
              JOB DESCRIPTION - RESPIRATOR SPECIFICATION FORM
JOB
DESCRIPTION
Contaminant                Concentration X.  ppm   mg/m3



Recommended Respiratory Protection


   First Choice	Second Choice	
NIOSH Approval Numbers



OSHA Standard for Contaminant
This form and the card that states that the employee is able to wear a respirator should be presented by
the employee at the time of the fit testing.  The form should also be filled out by the Public Health
Service and by the  person conducting the  fit test and forwarded to the Regional Health and Safety
Manager.

-------
                                                      SECTION:      APPENDIX A
                                                      VERSION:      FINAL/MAY 1997
                                                      PAGE 23 OF 23
Employee
                        RESPIRATOR ISSUANCE AND TRAINING
            Employee #
       Title
Date
Respirator:
Model
_Self-Contained
_Powered Air
_Dust/Mist Filter
 Dust/Fume/Mist Filter
	Supplied Air
_HEPA Filter
	Chemical Cartridge
	Chemical Cartridge w/pre-filter
     Application
NIOSH Approval #
Limitations:   Beard    Denture    Glasses    None
Explain:
Fitting:        	    Satisfactory
                     Positive Pressure
                     Test
                         	    Satisfactory
                         Isoamyl Acetate Test
                     Satisfactory
                     Negative Pressure
                     Test
                                Satisfactory
                                Sweetener Test
Maintenance: Cleaning:	Daily 	Weekly 	Other
              Disposal:	Daily 	Weekly 	Other
              	Individual 	Plant 	Other

Indicator
Employee Signature
                                       Date
Approved
                                       Date

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                                                          SECTION:  APPENDIX B
                                                          VERSION:  FINAL/MAY 1997
                                                          PAGE:  1 of 4
APPENDIX B: MEDICAL MONITORING PROGRAMS

MEDICAL MONITORING SELECTION POLICY

The Regional Medical Monitoring Selection Policy is detailed in Regional Order 3130, see Safety Office,
and is to be followed by all EPA Region 5 employees to which  it applies.


PURPOSE

The purpose of the Regional policy is to insure consistency in our approach to mandatory  participation
in Medical Monitoring and to ensure that we provide the most comprehensive health protection possible
for our employees. Baseline and subsequent yearly periodic medical monitoring exams are required for
three  categories of Regional employees.
POLICY

The three categories for which Medical Monitoring is mandatory are Hazardous Waste Site Workers,
Laboratory Personnel, and Other Field Personnel or Inspectors who are exposed to toxic materials. These
categories are detailed within the Procedure section below.
PROCEDURE

The first category of employees, Hazardous Waste Site Workers, are those who are engaged or involved
in clean-up operations, investigations, inspections, corrective actions or similar activities at hazardous
waste sites, treatment, storage, and disposal (TSDs) facilities or emergency response operations involving
hazardous substances. Regulations relative to Medical Monitoring requirements which apply to Hazardous
Waste Site Workers can be found in both 29 CFR 1910.120, Hazardous Waste Operations and Emergency
Response, Final Rule, dated March 6, 1989,  and EPA Order 1440.2.

The second category of employees required to participate in the Regional Medical Monitoring Program
is Laboratory Personnel who regularly work with  or  around  toxic substances which have a risk of
impairing  the health of the employee.  The regulations covering Medical Monitoring for  Laboratory
Personnel  are set out in the
EPA Order 1440, Chapter 8.

The third group of employees covered by mandatory Medical Monitoring consists of Field Personnel who
are routinely engaged in field activities (other than Hazardous  Waste Site activities) that might expose
them to toxic substances or  involve significant physical exertion. These employees are field inspectors
and other personnel who work with toxic substances, or  in the area of toxic substances to the  degree that
there is a risk of health impairment,  or who  frequently  engage  in arduous or physically taxing activity,
or who use respiratory protective equipment.

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                                                          SECTION:  APPENDIX B
                                                          VERSION:  FINAL/MAY  1997
                                                          PAGE:  2 of 4
All other personnel who may be exposed to some or all of the hazards and conditions described in this
Order, but to a lesser degree than the three categories of employees described herein, are encouraged to
enroll in the Regional Medical Monitoring Program.  Final determination, if a question arises relative to
individual participation or application of other related requirements relative to Medical Monitoring, will
be made by the Regional Safety Manager who will consult with U.S. PHS Personnel, as appropriate.  The
determinations made in this regard are important. A determination that an employee is in the mandatory
category of Medical Monitoring is also a determination that Medical Monitoring is a condition of
employment in that position.  Refusal  to  comply with this requirement  could represent grounds for
disciplinary action up to removal.
FURTHER REFERENCES

The regulations relative to Medical Monitoring for the personnel mentioned in this Order are to be found
in EPA Order 1440.2, see Safety Office.
MEDICAL MONITORING PROGRAM PROCEDURES

U.S. EPA, Region 5, has established an agency-wide medical surveillance program to reduce insofar as
possible the health risks of its employees who may be exposed to toxic substances or hazardous materials.
This program is designed to monitor the health of employees whose work regularly or periodically poses
the possibility of exposure to hazardous materials.  Representative job categories that have health
monitoring made  available to them  include  laboratory  workers.   Generally,  administrative, fiscal,
secretarial, and other support personnel who have only indirect, infrequent, or inconsequential incidental
exposures are not included.

All personnel who work (regularly or periodically) at Superfund or RCRA waste sites are required, by law,
to participate in the medical surveillance program as a condition of employment.  (29  CFR 1910.120)
Other employees  whose jobs justify  inclusion in the program  are free to participate  if they choose,
although EPA recommends  participation by all employees who are referred  for an examination.

Health monitoring is an employer responsibility and EPA bears the entire cost.  When a preexisting or
non-job related condition is detected in the course of a health monitoring examination, the employee is
referred to his/her private physician for further evaluation, treatment, and follow-up.  The employee must
bear these costs.   If the condition is later determined to have resulted  from  employment, the employee
may seek compensation and recovery of medical expenses from the  Department of Labor, Office of
Workers' Compensation Programs.

The health provider source for EPA  employees is the U.S. Public  Health Service (PHS), Division of
Federal Employee Occupational Health (DFEOH) by contractual arrangements. The PHS/DFEOH health
units are located in the Metcalf Federal Building at 77 W. Jackson and the Federal Office  Building at 536
S. Clark Street (Chicago); in the McNamara Building (Detroit); and the federal Office Building (Akron).
Consultation is available with the Regional Occupational Medicine Officer when  a job-related illness is
detected or suspected.

-------
                                                         SECTION:  APPENDIX B
                                                         VERSION:  FINAL/MAY 1997
                                                         PAGE:  3 of 4
CONTENT OF MEDICAL SURVEILLANCE EXAMINATIONS

Medical surveillance  consists of a series of blood chemistry tests, urine tests, and electrocardiogram,
pulmonary function testing, vision tests, hearing testing when there has been or might be exposure to
noise, cervical cytology (Pap smear test) and pelvic examination (females), medical and work history, and
a complete examination by a physician.  Chest X-rays and other special tests may also be included as
necessary. The physician is responsible for explaining the significance of all  findings - no employee
should leave the health unit with unanswered questions or concerns.
FREQUENCY OF MEDICAL SURVEILLANCE EXAMINATIONS

Baseline or pre-placement examinations are conducted prior to a job assignment where exposure to toxic
substances or similar hazardous materials may be possible.

Annual examinations are conducted for all persons in the job categories previously described.

Termination examinations are conducted at the termination of employment or before reassignment to an
area where medical examinations are not required, if an employee has not had an examination within the
last six months.

Crisis monitoring will  be provided to EPA employees who have experienced acute exposures or other
work  related  health  hazards, or who report aggravated  pre-existing medical  conditions as a result of
adverse work activities, on an emergency basis. Under these conditions, the employee may report directly
to the PHS health unit for  special tests as  necessary.   For  life threatening emergencies  and  for non
emergencies occurring  outside of normal working hours, the nearest hospital-based emergency center is
the most appropriate provider of the  required  services.   Prior EPA approval for medical testing is not
required under emergency circumstances; however, an application for  EPA approval must be submitted
promptly to the EPA.
ACCESS TO MEDICAL SURVEILLANCE EXAMINATIONS

The EPA Regional Health and Safety Officer develops a list of employees who are to be examined and
provides these names to the PHS health units.  The decision as to whom is recommended for the program
often  rests with the Branch Chief or supervisor most familiar with the possible hazards involved for
employees under their supervision.  If an employee has not been  included in the medical surveillance
program  and believes they may  have been exposed  to toxic substances or hazardous materials, the
supervisor should be contacted.

Upon enrollment in the medical surveillance program, the employee will  be contacted by the PHS health
unit nurse to schedule an appointment. Various forms shall be obtained from the Health and Safety Office
and must be  completed before the  actual examination -  a medical and work history (Baseline or Interim),
Privacy Act  Statement, Authorization for Disclosure of Information (to EPA Regional Health and Safety

-------
                                                         SECTION:  APPENDIX B
                                                         VERSION:  FINAL/MAY 1997
                                                         PAGE:  4 of 4
Officer for occupationally related findings only), and a medical questionnaire for respirator users.  It is
requested that the supervisor complete two forms: a "Request for Medical Clearance for Respirator Use"
(top portion) and a "Report of Employee Exposure to Hazardous Substance or Conditions". All forms will
be provided to the employee by an EPA liaison person or the PHS health unit nurse.

The medical surveillance examination is normally accomplished in two visits to the health unit. During
the first visit, basic tests are administered and samples of blood and urine are submitted to the laboratory.
(NOTE: NO DRUG TESTING OR AIDS TESTING IS PERFORMED.) A return visit is scheduled with
the physician when all test results have been received.

EMPLOYEE HEALTH MAINTENANCE EXAMINATION PROGRAM (EHMEs)

EPA's policies on employee health maintenance are described in a Regional Order titled "Employees
Health Maintenance Policy and Procedures."   The order  describes the available services under  the
Employee Health Maintenance Program.  The program provides  periodic examinations to evaluate  the
health of employees who are not clinically ill. It is designed to discover previously undiagnosed diseases
and to assist employees and organizations in maintaining optimum on-the-job health for their employees.
EPA, Region  5 participates in this program through  the Department of Health and Human  Services,
Division of Federal Employee Occupational Health.  The order also describes the process for  selecting
candidates for  inclusion in the  program and the criteria used in determining eligibility. The examination
consists of blood tests, hearing tests, vision screening and glaucoma tests, spirometry (a test for evaluating
the condition of the lungs), an EKG (a test to  evaluate the condition of the heart),  a pap smear (for
women), a proctoscopy of the colon (optional), and a full body physical examination.  An employee may
not receive an  EHME  exam more often than once within a two-year period.
OTHER HEALTH SERVICES PROVIDED TO EPA EMPLOYEES

Occasionally, there is confusion between the medical surveillance examination and the EHME-Employee
Health Maintenance Examination.  EHMEs are routine general medical exams, not related specific work
exposures.  As a participating agency in the PHS/DFEOH program, E;PA is allotted a certain number of
EHMEs per year. EPA, in turn, provides names of employees who are to receive EHMEs to PHS based
on a set of criteria developed by EPA. The EHME and the medical surveillance examinations are
performed for different reasons and should be viewed as two separate programs.

ATTACHMENT

Descriptions of the types of medical evaluations, as well as the appropriate medical surveillance forms,
are found in the Attachment.

-------
              UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

                         WASHINGTON, D.C. 20460
                          NCN  6 1995

                                                           OFFICE OF
                                                          ADMINISTRATION
                                                          AND RE SOURCES
MEMORANDUM                                                 MANAGEMENT
SUBJECT:  Occupational Medical  Surveillance Forms
           (TV^-    •   swl^
FROM:      Leah  4/. Henry/ HQOB
           Safety, Health & Environmental Management Division

TO:        ALL SHEMP Managers

     Attached please  find both  a  camera-ready  copy of  the new
Occupational  Medical Surveillance  (OMSP)  form as well  as a disk
containing the  form.  This form will be used for all EPA OMSP exams
beginning  January 1, 1996.  Please note that the use of this form
is mandatory.

     Those of you who have existing agreements with the Division of
Federal  Occupational Health  (DFOH) ,  U.S.  Public Health Service,
should  note  that DFOH  personnel have been  advised  that EPA will
require  the new form for all  OMSP exams.  However, your particular
physician  may not have  been made aware of the change.  In any case,
you  will need to modify your agreement with DFOH to specify the use
of this  form.  Those of you who  use private providers will need to
initiate a modification to your contract.  I suggest  that you begin
this process immediately  in  order  to meet the deadline.  Keep in
mind that  the provider will  be required to send completed  forms to
our  Medical  Review Officer  (at  present, Dr. Christopher Holland)
for  final  review.

      The transition to a standardized form is the second step (the
 first being  the development  of  a national policy) in our overall
effort to collect meaningful OMSP  data that will prove useful to
the   Agency   in  recognizing  and remediating real  and potential
exposures.  Additionally, the forms represent a major step towards
 limiting  OMSP   exams   to  those  employees  who need  them  and
 eliminating  employees  who do  not require  medical surveillance.

      We ask  for the cooperation  of everyone in the  implementation
 of  this change.  Please call me at  202-260-6340 if you have any
 questions  regarding   this   memorandum.    Thank  you  for  your
 assistance.
                                                         n.	i_

-------
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             TYPES OF EVALUATIONS - DEFINITIONS

BASELINES EVALUA TION -  The purpose of this evaluation is to characterize the
state of the individual prior to commencing work in a new assignment. It may also
be conducted in order to assess the individual's health status in relation to the
special demands of the proposed job assignment.

PERIODIC EVALUA TION - The purpose is to identify and measure any adverse
effects from occupational activities, and to control risks from occupational
exposures. These are conducted either annually or biennially.  The frequency is
based on specific criteria.

EXIT EVALUATION - This evaluation is conducted when someone terminates
employment in a given position that requires medical surveillance.  The purpose of
the examination is to document the health status of the individual at the end of
employment in that position.

SPECIAL EVALUATION - This evaluation is used to assess the health status of the
employee following an unusual exposure to a hazardous substance (biological,
physical, chemical, radiologic) or to evaluate medical issues relating to the
performance of specific job duties.   It may  also include follow-up testing. Please
note that an employee who  is acutely  symptomatic from an exposure to a
hazardous substance will be treated initially by their own physician or at an
emergency department.

-------
      CONTENT OF INDIVIDUAL MEDICAL EVALUATIONS

The content of any individual medical surveillance examination will include a "core"
component of data as well as data investigations unique to specific job duties and
exposures.

CONTENT OF "CORE" COMPONENTS:

1.    Medical and occupational history
2.    Physical examination
3.    Audio gram
4.    Vision testing
5.    Pulmonary function testing (periodicity established by age, smoking,
      type  and frequency of respirator use, known medical considerations
      and exposure profile)
6.    Chest x-ray (initially, when medically indicated and exit)
7.    Routine blood and urine analysis (includes complete blood count -
      CBC, blood chemistries - SMAC 20, urine analysis)
8.    Electrocardiogram (baseline at 40, and every five years thereafter)
9.    Tetanus vaccination (administered if employee has not had
      vaccination in 10  years)
10.   Cardiac stress test (only at the recommendation of the occupational
      medicine physician).  Generally this test should be obtained when the
      employee has multiple risk factors for cardiovascular disease, has
      known but poorly characterized cardiac disease, AND is involved in
      one of the following activities:

      a.     Heavy lifting

      b.     Extensively employs personal protective equipment that
            places increased strain on the heart and lungs (IE.
            Impervious  chemical protective clothing)

      c.     Involved in  emergency response or criminal investigation

-------
            FREQUENCY OF MEDICAL EVALUATIONS

The frequency of the medical evaluations shall be determined by the nature, type,
frequency and intensity of the potential exposures.  Using the following guidelines,
the frequency will be established by the EPA Safety, Health, and Environmental
Management Program (SHEMP) Manager in consultation with the employee's
supervisor and the designated occupational physician.

      1.     Annual evaluations are recommended for laboratory or
            field employees who meet the criteria for participant
            selection and who fit into any one of the four categories
            described below:

            a.    A field or laboratory employee who:  is over
                 age 40; or has significant medical problems
                 or findings on prior medical surveillance
                 examinations (e.g. insulin requiring diabetes,
                 cardiovascular disease, elevated  live enzyme
                 test, etc.)

            b.    An emergency response team member.

            c.    An employee whose position requires
                 engaging in field or laboratory activities
                 more than 30 days per year.

            d.    An employee with routine exposure to a
                 substance regulated by OSHA and requiring
                 annual medical examinations.

      2.     Evaluations every other year for those employees not
            fitting the criteria for annual examinations (e.g.  a healthy
            employee not exposed to a substance that OSHA
            requires annual surveillance examination and/or who
            visits various field sites an average of 30 or fewer days
            per year).

-------
      JOB CATEGORIES  FOR SPECIALTY  EXAMINATIONS

A.    Office Workers
      BASELINE/EXIT - "Core Elements" ONLY
      PERIODIC - "Core Elements" ONLY

B.    Pesticide Laboratory Workers
      BASELINE/EXIT - "Core Elements, Serum Lead, Urine Heavy Metal Screen
      (Arsenic, Mercury, Bismuth,  and Antimony), Serum Polychlorinated Biphenyl
      (PCS), Red Blood Cell Cholinesterase, and Respirator Clearance
      PERIODIC - "Core Elements", Serum Lead, and Respirator Clearance

C.    Solid Waste/Emergency Response Team
      BASELINE/EXIT - "Core Elements", Red Blood Cell Cholinesterase, Urine
      Heavy Metal Screen (Arsenic, Mercury, Bismuth, and Antimony), and
      Respirator  Clearance.
      PERIODIC - "Core Elements", Serum Lead, and Respirator Clearance.

D.    Fiejd Sampling Personnel
      BASELINE/EXIT - "Core Elements", Serum Lead, Red Blood Cell
      Cholinesterase, Urine  Heavy Metal Screen (Arsenic, Mercury, Bismuth, and
      Antimony), and Respirator Clearance.
      PERIODIC - "Core Elements", Serum Lead, and Respirator Clearance.

E.    Clean Air Inspector/Enforcement Officers
      BASELINE/EXIT - "Core Elements" and Serum Lead
      PERIODIC - "Core Elements" and  Serum Lead

F.    FIFRA Enforcement Officers
      BASELINE/EXIT - "Core Elements" and Red Blood Cell Cholinesterase
      PERIODIC - "Core Elements" and  Red Blood Cell Cholinesterase

G.    Lab Employees
      BASELINE/EXIT - "Core Elements", Red Blood Cell Cholinesterase, Serum
      Lead, Serum Polychlorinated Biphenyl (PCB), Urine Heavy Metal  Screen
      (Arsenic, Mercury, Bismuth,  and Antimony), and Respirator Clearance (if on
      an emergency team).
      PERIODIC - "Core Elements", Serum Lead, and Respirator Clearance (if on an
      emergency team).

H.    NESHAPS/AHERA (Asbestos Enforcement Officers)
      BASELINE/EXIT - "Core Elements", B-reading, and Respirator Clearance
      PERIODIC  - "Core Elements", Chest x-ray (Follow instructions in the
      Occupational Safety Health Evaluation for Periodic Examinations) and
      Respirator Clearance.

-------
I.     NPDES Inspectors
      BASELINE/EXIT - "Core Elements" ONLY
      PERIODIC - "Core Elements" ONLY

J,     Radiation Staff
      BASELINE/EXIT - "Core Elements", Red Blood Cell Cholinesterase. Serum
      Lead, Stress EKG (if >40 years old), and Respirator Clearance.
      PERIODIC - "Core Elements", Red Blood Cell Cholinesterase, Stress EKG
      (Triennial Basis), and Respirator Clearance.

K.     Remedial Project Officers
      BASELINE/EXIT - "Core Elements", Red Blood Cell Cholinesterase, Serum
      Lead, and Respirator Clearance.
      PERIODIC - "Core Elements", Serum Lead, and Respirator Clearance.

L.     TSCA Enforcement Officers
      BASELINE/EXIT - "Core Elements", Red Blood Cell Cholinesterase, Serum
      Lead, Serum Polychlorinated Biphenyl (PCB), and Respirator Clearance.
      PERIODIC - "Core Elements" ONLY

M.    U.S.T. Inspectors
      BASELINE/EXIT - "Core Elements" ONLY
      PERIODIC - "Core Elements" ONLY

N.    Wetlands Staff
      BASELINE/EXIT - "Core Elements" and Lyme Titer IGG
      PERIODIC - "Core Elements" ONLY

0.    Divers
      Follow previously established protocol
Employees are required to notify the health center 48 hours in advance for
preliminary and physical examination appointment cancellations.  The agency will
be billed $34.00 for preliminary and $65.00 for physical examination appointments
that are not cancelled.

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                                                        SECTION:      APPENDIX C
                                                        VERSION:      FINAL/MAY 1997
                                                        PAGE 1 OF 3
APPENDIX C:  HEARING CONSERVATION PROGRAM

Under the OSHA regulation for Occupational Noise Exposure (29 CFR 1910.95), a program must include:

       • Assessment of noise exposure
       • Audiometric tests of exposed workers
       • Noise abatement and/or administrative controls
       • Availability of hearing protection
       • Employee training and education
       • Maintenance of records on noise and hearing data


Assessment of Noise Exposure

GLNPO  research vessels are required to follow the regulatory specifications as outlined  in the OSHA
standard  for Occupational Noise Exposure  (29 CFR 1910.95).

When information  indicates that a worker's exposure may equal or exceed an 8-hour Time-Weighted
Average (TWA) of 85 dBA, GLNPO must implement a monitoring program.  Monitoring of the worker's
noise exposure should be performed in accordance with the regulatory standard and must be computed
in accordance with Appendix A of the standard (29 CFR 1910.95).   Monitoring  shall be repeated
whenever a change in production,  process, equipment or controls change or increase noise exposures.
GLNPO will provide  affected employees the opportunity to observe results of noise measurements.

To determine if personnel are being over exposed to noise, sound level meters should be used to  identify
areas where hazardous noise  levels exist.   The sound level meter will measure noise at different times
during the day and indicate a daily average exposure.  Alternately, a noise dosimeter may be attached
directly to a worker to measure the amount of noise to which they are exposed during a period of time.
If noise levels are at 85 dBA, or higher, based on an 8-hour work day, GLNPO will notify the worker.


Audiometric Tests of Exposed Workers

29 CFR 1910.95  states that "The employer shall establish and maintain an audiometric testing program"
by "making audiometric testing available to all employees whose exposures equal or exceed  an 8-hour
Time-Weighted Average  (TWA) of 85 dBA."  This testing shall be provided at no cost to the employee.
Testing must be performed by a licensed or certified professional in accordance with 29 CFR 1910.95(g)
(3).  All  audiograms shall meet the requirements of Appendix C of the regulation.

Within six (6) months of the worker's first exposure at or above  85 dBA, GLNPO will provide the
employee with a baseline audiogram to which subsequent audiograms can be compared.  Audiograms shall
be repeated at least annually thereafter. Evaluation of the audiograms shall be performed in accordance
with 29 CFR 1910.95(g) (7-10).

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                                                          SECTION:      APPENDIX C
                                                          VERSION:      FINAL/MAY 1997
                                                          PAGE 2 OF 3
Noise Abatement and/or Administrative Controls

When workers are subjected to sound exceeding 85 dBA, administrative or engineering controls will be
implemented. If such controls fail to reduce levels to acceptable limits, personal hearing protection should
be provided.  Hearing protection can include the following:

       •   Ear plugs:   These seal the ear canal and prevent noise from reaching delicate parts of the ear.
           Ear plugs are inexpensive and portable, but they  can be hard to fit and can introduce dirt into
           the ear canal if not kept clean.

       •   Canal caps:  These are soft pads  on a headband that seal the entrance to the ear without
           entering the ear. If used incorrectly, they might not seal the ear properly.

       •   Ear muffs:  These have a head band, ear cups, and ear cushions.  This type of ear protection
           usually provides the greatest amount of protection.
Availability of Hearing Protectors

GLNPO will make hearing protection available to  all workers exposed to an 8-hour Time-Weighted
Average (TWA) of 85 dBA or greater.  This is provided at no cost to the worker and should be replaced
as necessary.

GLNPO will ensure that hearing protection is worn in compliance with the regulation.  In addition,
GLNPO will ensure proper selection of hearing protection

Based on the evaluation methods described in Appendix B of the regulation (29 CFR  1910.95).

Employee Training and Education

GLNPO will institute a training program for all workers who are exposed to noise levels at or above the
85 dBA Time-Weighted Average (TWA).  Training will be updated arid repeated annually. Training will
include the following:

        • the effects of noise on hearing
        • the purpose of hearing protection
        • the advantages and disadvantages of various types of protectors
        • instruction  on the selection, fitting, use and care of various types of protectors
        • the purpose of audiometric testing
        • description of audiometric testing procedures

In addition, GLNPO will make available in the workplace and on board all research vessels a copy of the
standard (29 CFR 1910.95).

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                                                      SECTION:      APPENDIX C
                                                      VERSION:      FINAL/MAY 1997
                                                      PAGE 3 OF 3
Maintenance of Records on Noise and Hearing Data

GLNPO will maintain records of all worker exposure records in accordance with 29 CFR 1910.95 (M)
of the regulation. Noise exposure measurement records will be retained for two (2) years. Audiometric
test records will be retained for the duration of the worker's association with GLNPO.

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                                                         SECTION: APPENDIX D
                                                         VERSION: FINAL/APRIL 1997
                                                         PAGE:  1 of 3
APPENDIX D:  HAZARD COMMUNICATION PROGRAM
Commitment

The U.S. EPA is firmly  committed to providing a safe and  healthy work environment  for all EPA
personnel  and contractors.  In  order to meet  this commitment,  all GLNPO  facilities and  Research
Vessels are required to comply with  OSHA's Hazard Communication standard, 29 CFR 1910.1200.
A Hazard Communication program shall be implemented as outlined herein.

A Hazard Communication Coordinator will be assigned and  will have the overall responsibility for
ensuring compliance by coordinating the following functions:

       • Maintenance of the hazardous chemical inventory.

       • Maintenance of a complete Material Safety Data Sheet (MSDS) file and
       current employee  MSDS notebooks.

       • Training of all employees regarding chemical safety.

       • Updating of training as new chemicals are added or processes are changed.

       • Labeling of all transfer and waste containers.

       • Dissemination of hazard information to outside contractors

A successful  Hazard  Communication Program requires the cooperation of  EPA and  contracting
personnel.  It is the duty of GLNPO to assure that hazard communication is given proper priority.  All
personnel are encouraged  to ask questions and  keep  informed so that GLNPO can maintain a safe and
healthy workplace.


Access To The Written Program

All, or any part, of this  written Hazard Communication Program is  available  to all EPA personnel,
contractors,  and/or their  designated  representative.   Interested persons can contact  the Hazard
Communication Coordinator for review and copying.

Labeling

In accordance with the OSHA Hazard Communication Standard (29  CFR 1910.1200), all containers
shall be labeled as specified in this section.

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                                                          SECTION:  APPENDIX D
                                                          VERSION: FINAL/APRIL 1997
                                                          PAGE:  2 of 3
A.     No hazardous chemicals will be accepted for use on board GLNPO research vessels or shipped
       to any outside location, unless labeled with at least the following information:

                 • Identity of the hazardous chemical(s)
                 • Appropriate hazard warnings for the chemicals(s)
                 • Name and address of the chemical manufacturer, importer, or other
                 responsible party

B.     All   spray  bottles or other transfer containers will be labeled  with  at  least the following
       information:

                 • Identity of the hazardous chemical(s)
                 • Appropriate hazard warnings for the chemical(s)

C.     The Hazard Communication Coordinator is responsible for reviewing labels and assuring that
       the label information is kept current.

D.     No label  is to be defaced or removed when material is received or in use.   Any containers
       with missing labels will be removed from service until proper labels are installed.

Material  Safety Data Sheets

A material safety data sheet (MSDS) containing the information  required  by 29 CFR  1910.1200 shall
be maintained for each hazardous substance. The MSDS shall be the  most current  one supplied by the
chemical manufacturer, importer, or distributor.  MSDSs will be available for review by all personnel.

The Hazard  Communication  Coordinator  shall  be  responsible for  maintaining  an  inventory  of
chemicals currently in use.  In addition, a  separate inventory file must be maintained of previously
used chemicals.  Inventory records of previously used chemicals must be maintained for thirty-years as
part of employee  exposure records.

An office  file  containing the current chemical inventory and appropriate  MSDSs is located  in the
laboratory office on board ship.  A copy of the inventory and MSDSs for use by occupants of GLNPO
facilities  can  be found in wall-mounted notebooks located near the point of use  in the hallway between
the Chemistry and Biology laboratories.

Personnel Information  and Training Policy

A.     All EPA personnel and contractors, including temporary contractors  who may  possibly  be
       exposed to chemical hazards shall  be appropriately informed  and/or  trained concerning the
       potential hazards of the chemicals in GLNPO facilities.

-------
                                                           SECTION:  APPENDIX D
                                                           VERSION:  FINAL/APRIL 1997
                                                           PAGE:  3 of 3
B. .    All EPA  personnel and contractors,  including temporary contractors, shall be  informed of the
       details contained in this program, including an explanation of the labeling system, the material
       safety data sheets, and how to interpret  and use this information.

C.     All EPA  personnel  and contractors,  including temporary contractors shall  be provided with
       additional training  when  new chemical  hazards are  introduced  or  prior to  performing
       non-routine tasks that could involve exposure to hazardous chemicals.

D.     When appropriate,  reinforcement of training will  be  conducted through  topics  at  safety
       meetings.

E.     The extent of  information  transmitted to EPA  personnel and contractors  during training
       sessions will be dictated by the degree  of chemical hazard present.  The following sources of
       information will be used during training sessions:

       • The text of the Hazard Communication Standard (29 CFR 1910.1200)

       • An inventory list of hazardous chemicals

       • Applicable MSDSs

       • The written Hazard Communication Program

Contractor Policy

Outside contractors shall be provided with all necessary information concerning the potential hazards
of the substances to which they may  be exposed and appropriate protective  measures required to
minimize their exposure.

Whenever possible, the  contractor  or  agency  management should be  provided with a  list of the
hazardous chemicals and  the  material safety data sheets  for the materials their employees may be
exposed to by virtue of their work in GLNPO facilities.  In addition, contractors must have MSDSs on
site for all potentially hazardous chemicals they  use or produce in GLNPO facilities.

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PERMIT-REQUIRED CONFINED SPACE ENTRY PROGRAM

-------
                                                        SECTION: APPENDIX E
                                                        VERSION: REVISION/MAY 1997
                          TABLE OF CONTENTS

I.     PURPOSE  ............................................  1

II.    IDENTIFICATION OF PERMIT-REQUIRED CONFINED SPACES .......  1

III.    POLICY  .............................................  2

IV.    PROGRAM RESPONSIBILITIES ..............................  2

V.    PROGRAM ELEMENTS ...................................  3
      Identification and Testing ................................ 3
      Ventilation and Personnel Protection Requirements ................ 4
      Training .......................................... 4
      Recordkeeping ...................................... 4
VI.    ENTRY PERMITS .......................................  5

VII.   PERSONAL PROTECTIVE EQUIPMENT (PPE) AND DEVICES  ........  5

VIII.  PRE-ENTRY PROCEDURE  .................................  6

IX.    PERMIT CLOSURE AND CANCELLATION  .....................  8

X.    RECLASSIFICATION PROCEDURES ..........................  8

XI.    SPECIAL EQUIPMENT  ...................................  8

XII.   RESPONSIBILITIES  ......................................  9
      Authorized Entrant's Duties  .............................  9
      Chief Engineer's Duties ............................... 10
      Attendant's Duties  .................................. 10

XIII.  RESCUE TECHNIQUES ...................................  12

XIV.  CONTRACTOR REQUIREMENTS  ............................  13
                             ATTACHMENTS
I.     Confined Space Entry Program Checklist
II.    Confined Space Entry Permit
III.    References

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                                                                SECTION: APPENDIX E
                                                                VERSION: REVISION/MAY 1997
                                                                PAGE, 1 of 13
 I.    PURPOSE

      The purpose of this program is to ensure that no one enters or works in a hazardous atmosphere
      contained in a compartment or space aboard GLNPO vessels.  In general, no routine hazard is as
      dangerous as the presence of potentially lethal atmospheres in vessel spaces.  By design, some
      vessels have contained spaces (especially tanks and voids) in which a multitude of both toxic and
      non-toxic gas  or vapor  creating  substances  are  used in the normal operation  of the vessel.
      Although in many instances harmful gases or vapors are  present in such low concentrations that
      no adverse conditions are created, hazardous atmospheres that have the potential to be created can
      explode or cause asphyxiation.  Compounding the problem is that many gases or  vapors are not
      detected by smell, and personnel attempting to save a fallen shipmate may themselves be overcome
      and killed by undetected vapors.  It is for these reasons that every confined space  must be tested
      for oxygen-deficient or otherwise hazardous atmospheres.
II.    IDENTIFICATION OF PERMIT-REQUIRED CONFINED SPACES

      A documented evaluation must be performed on all areas of GLNPO vessels that are or may be
      considered to be Permit-Required Confined Spaces.  The following criteria should be used to
      evaluate the vessel.

      A confined space is any enclosure or compartment that:

      1.   Has a limited means of exit or entry (for example, a hatch on a tank),
      2.   Is large enough to  enter and perform work, and
      3.   Is not designed for continuous employee occupancy.

      A permit-required space is a confined space that exhibits one or more of the following hazards:

      1.   Contains or has the potential to contain a hazardous atmosphere,
      2.   Contains a material that has the potential for engulfment (i.e., sand, grain, etc.),
      3.   Has the  internal configuration such that an entrant could be trapped or  asphyxiated by
           inwardly converging walls or by a floor which slopes downward and tapers to a smaller
           cross-section, or
      4.   Contains any other recognized serious safety or health hazard.

      Those places identified as permit-required confined spaces (permit spaces) must be identified with
      a sign that states:

                          "DANGER   PERMIT-REQUIRED
                      CONFINED SPACE,  DO NOT ENTER"

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                                                                    SECTION: APPENDIX E
                                                                    VERSION- REVISION/MAY 1997
                                                                    PAGE: 2 of 13
III.    POLICY
       Permit-required confined spaces shall not be entered by any U.S. EPA employee or contractor that
       is not specifically authorized to do so.  Written authorization to enter a permit space must be
       obtained from the GLNPO Occupational Health and Safety Manager and the Regional Occupational
       Health and Safety Manager concurrently.  No  individual  shall enter a permit space without
       following  all predetermined  and precautions  and procedures.   Only  personnel  trained  and
       competent in permit-required confined space entry shall be allowed to enter and/or perform work
       in a permit-required confined space.  Contact will be made with all emergency services prior to
       space entry.  Any violation of this policy may result in a serious accident or injury.  Therefore,
       any individual  found not  complying with established  procedures will be  subject to severe
       disciplinary action.
IV.    PROGRAM RESPONSIBILITIES

       A.  If the vessel design is such that there are confined spaces where toxic and non-toxic gases
            may accumulate, the vessel's Master is responsible for a comprehensive confined-space entry
            program.

            The vessel's Master must:

            •   Ensure that  there is at least one trained,  qualified and certified  gas-free engineer on
                board.  The  Chief Engineer shall be designated as the ship's gas-free engineer (GFE).

            •   Ensure that confined-space  entry practices  are established.

            •   Ensure that this program is evaluated annually for compliance and effectiveness.  The
                Confined-Space Program Entry Checklist (Attachment I) may be used for this purpose.

       B.  The Chief Engineer shall ensure that:

            •   All personnel that  will perform  permit-required confined space entries as either entry
                supervisor, authorized entrant, or authorized attendant are trained  concerning confined-
                space entry hazards, procedures  and  precautions.  Additional support may be obtained
                from qualified  marine  chemists  or industrial hygiene officers, certified as Gas-Free
                Engineers.

            •   All equipment  required for proper evaluation of confined  spaces is aboard the ship,
                inventoried annually, and properly  maintained.

            •   Gas-free certificates are posted in necessary areas.

            •   Records of gas-free space testing are  kept.

            •   Assigned personnel receive  training on the confined-space entry program upon reporting
                aboard and annually thereafter.

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                                                                   SECTION  APPENDIX E
                                                                   VERSION  REVISION/MAY 1997
                                                                   PAGE: 3 of 13
      C.   All hands are responsible to ensure that:

           •   The Chief Engineer is notified prior to entering any unventilated, non-occupied space that
               has been designated to store hazardous or toxic materials or any sealed space.

           •   The Chief Engineer is  notified prior to conducting hot work  on a bulkhead,  deck, or
               overhead, adjacent to a space containing flammable or potentially explosive atmospheres
               (such as fuel oil or contaminated holding tank).

           •   Gas-free engineering certificates posted on spaces are complied with at all times.

           •   Gas-free engineering retesting of spaces is accomplished prior to the end of the period
               for which a gas free certificate is valid.  If a change is  made to a space, the space shall
               be retested and recertified prior to any additional work in the space.

           •   Notify the  Chief Engineer prior  to  entering any unventilated, non-occupied space
               designated to store hazardous or toxic materials or any sealed space.  Verify that such
               a space was checked by a gas-free engineer prior  to entry, and  comply with the gas-free
               engineering certificates posted outside the space.

           •   Notify the Chief Engineer  before any new space  is used to store hazardous  or toxic
               material or of any spill of hazardous or toxic material.
V.    PROGRAM ELEMENTS

      The confined-space entry program consists of the following elements, selected on the basis of
      typical shipboard operations:
      1.
      2.
       3.
Identification and Testing.   Any sealed or unventilated,  unoccupied space designated for
hazardous or toxic materials storage must be tested for oxygen deficiency prior to entry.  Hot
work on  a bulkhead, deck, or overhead adjacent  to a space containing flammable or
potentially explosive atmospheres (e.g., fuel oil vapors) also required testing.

Ventilation and Personnel Protection Requirements.  Any sealed or unventilated, unoccupied
space designated for hazardous or toxic materials storage must be ventilated prior to entry.
During activities which require entry into confined spaces., one individual must always stand
watch at the entrance to monitor the worker(s) and notify appropriate rescue personnel in case
of emergency.

Training.  The Chief Engineer is  responsible for ensuring that assigned personnel receive
training on the gas-free engineering program upon reporting aboard and annually thereafter.
Records of such training shall be maintained.

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                                                                    SECTION: APPENDIX E
                                                                    VERSION: REVISION/MAY 1997
                                                                    PAGE: 4 of 13
            Additional training will be conducted when:

            a.  The job duties change,
            b.  There is a change in the permit space program or the permit space operation presents a
               new hazard,  or
            c.  When an employee's job performance shows deficiencies.

            Training will consist of the following topics:

            a.  The locations and hazards of the confined spaces aboard the vessel
            b.  Procedures for requesting gas-free testing
            c.  Proper permit completion
            d.  Duties of entrants, attendants, and the Chief Engineer
            e.  Pre-entry procedures
            f.  Personal Protective Equipment (PPE)
            g.  Procedures for helping shipmates in an emergency.  Training must stress to all personnel
               that if a person is seen unconscious in any space, no one is to enter that space without
               appropriate  respiratory protective  equipment  and a  backup  assistant. All  gas-free
               engineering personnel shall be trained in CPR and  emergency and rescue procedures.
            h.  Recordkeeping

            Retraining on all aspects shall be accomplished annually.

       4.   Recordkeeping.  All gas-free engineering tests must be recorded in the confined-space entry
            log which contains the following data:

               •   Date and time of inspections/tests.
               •   Location of confined space(s).
               •   Tests conducted and results.
               •   Prescribed instructions.
               •   Type of  entry or work performed.
               •   Name of person(s) conducting inspection/test.
VI.    ENTRY PERMITS

       A

            Safety Manager
I>1KY m,KlVlli»

..  No permit-required  confined space shall  be entered  without  a valid permit and written
   authorization from the GLNPO Health and Safety Manager and the Regional Health and
   Safety Manager.
       B.   The permit shall be issued and signed by the Chief Engineer, who shall act as the entry
            supervisor.

       C.   The permit will be completed in its entirety; there shall be no blanks.

       D.   The permit will be posted at the entrance of the confined space.

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                                                                    SECTION: APPENDIX E
                                                                    VERSION: REVISION/MAY 1997
                                                                    PAGE- 5 of 13
         E.   The Confined Space Entry Permit will be valid for a single work shift only.  On projects
             requiring more than a single work shift, a new permit shall be completed at the start of each
             shift.

         F.   The Confined Space Entry Permit is in Attachment II.

         G.   Canceled permits will be reviewed annually to determine the adequacy of the Program and
             procedures used.  Canceled permits must be retained for at least one year.
 VII.    PERSONAL PROTECTIVE EQUIPMENT (PPE) AND DEVICES

         Personal protective equipment, such as impervious gloves, boots and goggles, appropriate for the
         hazards of the space, must be used as specified on the entry permit.

         Each worker entering a permit space must wear a body harness with a lifeline attached, unless such
         equipment creates an additional hazard or does not contribute to the emergency rescue of a worker.
         A fall arrester and winch retrieval system is required for vertical confined space entry work deeper
         than five  feet.  Training in proper use of the retrieval device may be prov|ded by the equipment
         vendor.

         Respiratory protection  may be  required  under special circumstances.  Only those employees
         specifically trained in respiratory protection may wear respirators.

         Entry into some confined spaces, such as compartments in the lower areas of vessels, may require
         that entrants wear  respirators attached to  supplied breathing air lines, or, at a minimum, have in
         their immediate possession, ready for use, a respirator attached 1o a five-minute emergency escape
         breathing air canister.   Emergency canisters, if supplied, are riotto be used while working in the
         confined  space, but only  for escape, should it become necessary.  Such an entry  is prohibited
         without written authorization from the GLNPO Occupational Health and Safety Manager and the
         Regional  Occupational Health and Safety Manager.
VIII.    PRE-ENTRY PROCEDURE

         Before any employee is allowed to enter a confined  space, supervisory authorization shall be
         obtained and a Confined Spaces Entry Permit (see Attachment II) shall be initiated.

         •   Appropriate gas-free testing must be performed by qualified personnel from outside of the
             confined space to determine if a hazardous atmosphere exists.

         •   If tests indicate the atmosphere is initially safe  but the  work may produce a hazardous
             atmosphere from activities such as welding or the use of solvents, entry without respiratory
             protection will only be permitted if continuous atmospheric testing during entry is conducted
             by trained personnel.

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                                                             SECTION: APPENDIX E
                                                             VERSION- REVISION/MAY 1997
                                                             PAGE. 6 of 13
•    If tests indicate that the atmosphere is unsafe, the confined space shall first be ventilated until
     the hazardous condition has been removed and confirmed by testing.  The confined space shall
     be thoroughly ventilated.  Special care should be taken to assure that circulated air will reach
     isolated pockets and recirculating contaminated air is prevented. Provisions shall be made for
     adequate  make-up air during the entire entry, using an external blower with a flexible hose.
     Be aware that exhausted air may contain hazardous vapors or gases.

•    The confined space shall be ventilated using an external blower with flexible hose to prevent:

        - oxygen excess or deficient atmospheres.
        - flammable vapors in the atmosphere above  10% of the LEL,
        - toxic vapors, other contaminants, gases, or particulate accumulation, or

•    The employee entering the space must wear protective clothing appropriate for the anticipated
     hazards.  Refer to the Special PPE  section of the permit and to the Material Safety Data Sheet
     (MSDS) for the contaminant, if necessary.

     Unless otherwise specified authorized entrants who enter a permit space must wear a chest or
     full body harness with a retrieval line attached to the center of their backs near shoulder level
     or above their heads.  The other end of the retrieval  line must be attached to a mechanical
     device or to a fixed point outside the permit space.

     A mechanical device must be available to retrieve entrant from vertical type permit spaces
     more than five feet deep.

•    The space shall be isolated. All incoming piping and  lines will be blinded, bled, and locked.
     The use of an in-line shut-off valve as the  sole means of isolating the confined space from
     any line  is  prohibited.  All  electrical, hydraulic,  and  pneumatic  powered  machines  and
     equipment shall be de-energized and locked out.  All stored or accumulated energy shall be
     dissipated.

•    Every effort shall be made to make the confined space as safe to enter as possible. This may
     require purging, ventilation,  pumping, flushing, etc. from a remote position outside the
     confined  space. Lighting shall be adequate for the  work being performed.

•    The Confined Space Entry Permit will not be issued unless the following provisions have been
     instituted:

     A. An attendant must maintain constant vigilance and maintain verbal communications with
        the worker inside.   When using the  single observer  method  the attendant must be
        equipped with a means of obtaining help without leaving  the area (radio, airhorn, etc.).
         Surrounding personnel must be aware of this procedure and be trained to respond.

     B. Adequate rescue procedures must have  been developed and practiced (see section XIII,
        Rescue Techniques).

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                                                                  SECTION: APPENDIX E
                                                                  VERSION: REVISION/MAY 1997
                                                                  PAGE. 7 of 13
            C.  The attendants shall be trained in their duties (see Section XI, C, Attendant's duties).

            D.  Employees must be aware that they should NEVER attempt to enter a confined space to
               rescue an individual who needs help, unless trained and equipped to do so.

            E.  All nearby and entry personnel shall understand the hazards of the confined space and be
               instructed about precautions necessary.
       •    Access openings, hinged doors or hatch  covers shall be secured open and  guarded  from
            hazards outside the space.

       •    The completed permit shall be posted in a conspicuous place near the point of entry.


IX.    PERMIT CLOSURE AND CANCELLATION

       The permit shall be closed and/or canceled by the following situations:

       •    At the completion of the  specified work or operation specified on the permit.
       •    At the end of each work  shift.
       •    Any condition that is not allowed under the entry permit arises in or near the permit space.

       The  closure of the permit will  include closing  notes detailing  any  additional  practices and
       procedures that were used to eliminate or mitigate new hazards or problems encountered during
       the entry.


 X.    RECLASSIFICATION PROCEDURES

       A confined space that has been designated a Permit-required confined space may be reclassified
       as a non-permit space if the following provisions are met:

       •    Written authorization for reclassification must be obtained  from the GLNPO Health and
            Safety Manager and the Regional Health and Safety Manager.

       •    The space must pose no actual or potential atmospheric hazards.  Hazardous  work or  work
            that may create a hazardous atmosphere, e.g. painting, welding , etc., may not  be performed.
            Controlling a hazardous  atmosphere with ventilation does not constitute the  elimination of
            atmospheric hazards.

       •    All other hazards associated with the space must be eliminated.   An example of this may
            include locking  out the power source to an unguarded fan that is  in the space.

       •    If it is necessary to enter a permit space in order to eliminate the hazards of the  space, a
            Permit must be  issued and standard permit space entry procedures must be followed.

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                                                                    SECTION: APPENDIX E
                                                                    VERSION: REVISION/MAY 1997
                                                                    PAGE: 8 of 13
        •    Only the Chief Engineer shall be allowed to reclassify a permit space as a non-permit space.
             The basis for determination that all hazards have been eliminated shall be documented by the
             Chief Engineer.  This certification shall be signed, dated, and posted at the space entrance.

        •    If any  hazard  arises inside  the space during  the course of work then all entrants  must
             immediately evacuate the space. The space shall not be reoccupied until the hazard has been
             eliminated and the space has been re-evaluated.
   XI.  SPECIAL EQUIPMENT

        A.   Portable Tools

             All portable power tools and extension cords are to be grounded, in good repair and protected
             by a ground-fault circuit interrupter (GFCI).

        B.   Ladders

             A ladder may be used as a means of entering and exiting, provided it does not obstruct the
             opening.  A ladder is required in all confined spaces deeper than four feet.  The ladder shall
             be secured and not removed until all employees have exited the space.

        C.   Lighting

             Low voltage lighting is suggested,  such as a 12 volt  florescent lantern.

        D.   Non-sparking Tools and Equipment

             Anytime  that there is a reasonable  potential of an explosive or flammable atmosphere only
             non-sparking tools and explosion proof equipment shall be used.


XII.    RESPONSIBILITIES

        A.   Authorized Entrant's Duties

             •   Know the hazards of the confined space, including information on the mode of exposure
                 (e.g., inhalation or skin  absorption), signs or  symptoms,  and consequences of the
                 exposure;

             •   use appropriate personal protective equipment properly (e.g., face  and eye protection,
                 and other forms of barrier protection such as gloves,  aprons, and coveralls);

             •   maintain communication (i.e.,  telephone, radio,  visual observation) with attendants to
                 enable the attendant to monitor the entrant's status  as well as to  alert the  entrant to
                 evacuate;

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                                                             SECTION: APPENDIX E
                                                             VERSION: REVISION/MAY 1997
                                                             PAGE: 9 of 13
•    exit from the  permit  space as  soon as possible  when  ordered by an  authorized person
     (supervisor and/or attendant), when the entrant recognizes the warning signs or symptoms
     of exposure, when a prohibited condition exists, or  when an automatic alarm is activated; and

•    alert the attendant when a prohibited condition exists or when warning signs or symptoms of
     exposure exist.
B.   Chief Engineer's Duties

     1.  Routine Duties

        •   Know the confined space iiazards, including information on the mode of exposure,
            signs, or symptoms and consequences of exposure;

        •   verify  emergency plans and  specified entry conditions  such as  permits,  tests,
            procedures, and equipment before allowing entry:

        •   take appropriate measures to keep unauthorized personnel away from the site;

        •   ensure that entry operations  remain consistent  with the entry  permit  and that
            acceptable entry conditions are maintained; and

        •   terminate entry and cancel permits when entry operations are completed or if a non-
            permitted condition develops.

        •   Establish the  rotation time cycle (duration) for hot locations that can  result  in
            excessive heat stress on the entrant and enter this time on the permit.

     2.  Emergency Duties

        •   In the event of an emergency,  the attendant shall summon aid  immediately and
            instruct the person who responds to  call  the listed  rescue service on the nearest
            phone. The supervisor shall ensure that rescue personnel are met at the site and led
            to the appropriate place.

        •   In the event that the rescue  is  to be  made by an  outside emergency  response
            organization, the supervisor must notify the vessel's Master of the event and details.
            If any injuries  occur,  the supervisor must also activate the accident reporting
            procedure.

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                                                             SECTION. APPENDIX E
                                                             VERSION: REVISION/MAY 1997
                                                             PAGE: 10 of 13
C.   Attendant's Duties

     1.   Routine Duties

         •   assure that the appropriate equipment is operable and available at the work site;

         •   know the location of the nearest emergency alarm or phone, the emergency number
            to be  used and the exact location of the work site;

         •   understand the present and potential hazards of the confined space;

         •   remain outside permit  space during  entry operations unless  relieved by another
            authorized attendant;

         •   perform non-entry rescues when specified by employer's rescue procedure;

         •   know existing and potential hazards, including information on the mode of exposure,
            signs  or symptoms, consequences of the exposure, and their physiological effects (for
            example, drowsiness, heat stress, twitching, or unconsciousness),  and know that
            entrants must leave the confined space when they exhibit symptoms of overexposure;

         •   maintain communication with and keep an accurate account of those entrants entering
            the permit-required space;

         •   order evacuation of the permit space when a prohibited condition exists, when an
            entrant shows signs of physiological effects of hazard exposure, when an emergency
            outside the confined space exists, or when the attendant cannot effectively and safely
            perform required duties;

         •   summon rescue and other services during an emergency;

         •   ensure that unauthorized persons stay away from permit spaces or exit immediately
            if they have entered the permit space;

         •   inform authorized entrants and Chief Engineer of entry by unauthorized persons; and

         •   perform no other duties that interfere with the attendant's primary duties.


     4.   Emergency Duties

         •   In the  event of an emergency, the  attendant shall summon aid immediately and
            instruct  the person who responds to call the listed rescue service on the nearest
            phone.

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                                                                     SECTION: APPENDIX E
                                                                     VERSION. REVISION/MAY 1997
                                                                     PAGE: 11 of 13
                     In the event that  the  rescue  is to be made by an outside emergency response
                     organization, the attendant is to take the following action:

                     1.   monitor the situation in order to give a detailed report to the emergency response
                         personnel,
                     2.   assist the emergency response personnel, if necessary, and
                     3.   when the situation is under control, notify the vessel's Master of the incident.
XIII.    RESCUE TECHNIQUES

         Rescue

         •    All entrants are required to wear a full body harness and life line when entering a permit
              space.  In all spaces with a greater depth than five feet, a mechanical retrieval unit must be
              used.

         •    Permit space entries should be reported to the designated rescue team to allow for preparation
              and reduce response time.

         •    The attendant shall sound an alarm and request that the responder call the site emergency
              number for additional assistance.

         •    The attendant shall attempt to remove the entrant while remaining outside of the confined
              space. If the rescue is not successful within 30 seconds, the attendant must call for additional
              help.

         •    Once the entrant has  been removed from the confined space,  the entrant must be moved to
              fresh air and any necessary  first aid must be applied by rescue personnel  only.

         •    If an injury occurs within a  confined space and the injured party is conscious but immobile,
              attendant should summon trained rescue personnel to remove the entrant and maintain safety
              watch.

         •    If an  injured entrant is exposed to a substance for which a Material  Safety  Data Sheet
              (MSDS) or other similar written information is required to be kept on board, that MSDS or
              other  written information must be made available to the emergency  personnel treating the
              exposed entrant.

         •    The attendant must understand that he/she is NEVER to go into a confined space to rescue
              an entrant.

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                                                                      SECTION: APPENDIX E
                                                                      VERSION: REVISION/MAY 1997
                                                                      PAGE: 12 of 13
          Training and Practices Rescue

          Rescue technique practice using the equipment described is vital.  These rescue techniques must
          be learned and reviewed by employees so they are skilled and comfortable in performing staged
          emergencies.  Annual training should include mock emergencies using actual permit spaces and
          equipment.  Drills should be coordinated with rescue teams.
XIV.     CONTRACTOR REQUIREMENTS

          When outside contractor services are required to enter a permit space, GLNPO shall:

          •    Inform the contractor of the presence of permit-required confined spaces at the site and that
               entry is allowed only through adherence to this program.

          •    Apprise the contractor of the hazards and precautions associated with the spaces they are to
               enter.  This may be accomplished by reviewing the requirements of the entry permit with the
               contractor,  and assisting the contractor in the completion of the permit.  The contractor
               should use this information as a minimum guideline only and should take all the precautions
               over and above the guideline to ensure safe space entry.

          •    Debrief the contractor at the conclusion of the entry operation.  All information relating to
               the space entry including  atmospheric  conditions, additional hazards encountered, and the
               precautions taken to address the hazards must be noted on the canceled permit used by the
               contractor.

          •    Coordinate  entry operations when entrants from both parties  are entering and  working in
               permit spaces concurrently.  The following requirements are recommended:

               A. Information should be exchanged  including specific work being  performed, hazards
                  created  by the work, and all precautions used.

               B. Separate permits may be used and completed separately.

               C. Both parties shall ensure that isolation procedures are  completed and separate locks and
                  tags must be used.

               D. Separate attendants may be used and must remain in constant communication with each
                  other.

               E. Contractors are responsible for providing their own equipment. GLNPO will not provide
                  any  equipment for any contractor.

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                                                            SECTION: APPENDIX E
                                                            VERSION: REVISION/MAY 1997
                                                            PAGE: 13 of 13
All contractors shall:

•    Obtain information from GLNPO about the presence of permit-required confined spaces on
     board and that entry is allowed only through adherence to this program.

•    Coordinate entry operations when entrants from both parties  are entering and working in
     permit spaces concurrently.

•    The  contractor shall  inform GLNPO at the conclusion of the  entry  operation  of all
     information relating to the space entry including atmospheric conditions, additional hazards
     encountered, and the precautions taken to address the hazards.

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                                     SECTION APPENDIX E
                                     VERSION: REVISION/MAY 1997
          ATTACHMENT I

Confined Space Entry Program Checklist

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                                                                SECTION: APPENDIX E - ATTACHMENT I
                                                                VERSION: REVISION/MAY 1997
         CONFINED-SPACE ENTRY PROGRAM CHECKLIST
This checklist should be used to evaluate the effectiveness of the confined-space entry program.


1.    Is there at least one trained, qualified, and certified gas-free engineer aboard?

2.    Is gas-free engineering equipment properly maintained?

3.    Is gas-free engineering equipment inventoried annually?

4.    Are gas-free certificates posted in necessary areas?

5.    Are records kept of space gas-free testing and are these records audited annually?

6.    Is the Chief Engineer notified before anyone enters any unoccupied space that is not designed to be
     ventilated during routine operations, that has been designated to store hazardous or toxic materials, or
     that is normally  sealed under  routine operating conditions?

7.    Is the Chief Engineer notified before hot work is conducted on a bulkhead, deck or overhead adjacent
     to a space containing flammable or potentially explosive atmospheres (such as fuel oil or contaminated
     holding tank)?

8.    Is the Chief Engineer notified of the stowage in a new location or spill of any hazardous or toxic
     material?

9.    Are gas-free engineering certificates posted on spaces complied with at all times?

10.  Is gas-free engineering conducted whenever anchorage is made to a space under a gas-free engineering
     certificate prior to additional work occurring in the space?

11.  Are periods for which gas-free certificates are valid posted on each  certificate?

12.  Are spaces retested prior to the end  of the period for which a gas-free certificate is valid?

13.  Does the gas-free engineering  test log document all gas-free engineering tests and contain the following
     data:

              Date and time of inspections/tests?
              Location of space?
              Tests conducted and results?
              Prescribed instructions?
              Type of entry or  work to be performed?
              Name  of person conducting test/inspection?

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                                                                  SECTION: APPENDIX E - ATTACHMENT I
                                                                  VERSION: REVISION/MAY 1997
14.   Are gas-free engineering tests conducted in the order of oxygen, combustible gases, toxic gases?

15.   If gas-free engineering tests indicated the presence of a flammable vapor, was the space ventilated until
     vapor was no longer detectable?

16.   Do all hands receive training on the Confined Space Entry Program upon reporting aboard and annually
     thereafter?

17.   Does training cover the following:

               How to identify confined/enclosed spaces?
               Ha/irds encountered when entering confined/enclosed spaces?
               Procedures for requesting gas-free testing/
               Procedures for helping shipmates in an emergency?

18.   Are all personnel trained in CPR and emergency rescue procedures annually?

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                                SECTION: APPENDIX E
                                VERSION: REVISION/MAY 1997
    ATTACHMENT II

Confined Space Entry Permit

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                                                                  SECTION: APPENDIX E - ATTACHMENT  I
                                                                  VERSION. REVISION/MAY 1997
                             CONFINED SPACE ENTRY PERMIT
(CHECK OR FILL IN ALL BLANKS THAT APPLY)

TODAY'S DATE:	

TIME of ENTRY:
                   Date:	

PURPOSE OF ENTRY:       Inspection:.
BRIEF DESCRIPTION OF PURPOSE. _

LOCATION of ENTRY (Be Specific):
Name
        The U.S. EPA Personnel
                      Time In
    Time:
Time Out
      Safety Permit No.:
                                                 Other:
                                                 or
Name
Subcontractors Entering
           Time In     Time Out




HAVE AM. PERSONNEL GONK THROUGH A PRF-ENTRY RRIFFIN(T? Yes
SPACE TESTED BY (Name/Title)
TYPF # SERIAL NUMBER of SAMPLING EQUIPMENT' Make1
TYPE & SFRIAI. NIIMBFR of SAMPLING FOIIIPMENT: Make:
PRE-ENTRY READING TAKEN? Yes No
% Oxygen: Opening Middle Bottom
% LEL: Opening Middle Bottom
Toxic Reading: CO H2S Other
ARE ALL POWER SOURCES INTRINSICALLY SAFE? Yes
HAVE AI I PFRSONNFI. BFFN GIVFN THF PROPFR PPF? Yes

Date:
S/N
S/N
DOCUMENT
Other
Other
All PEL
No
Nn
Nn
Timp-
Talih Harp-
Talih Dafp-
LATER READINGS ON BACK
All 19.5%
All 10% LEL

HAVE ALL PERSONNEL BEEN TRAINED TO WORK SAFELY AND TO USE EQUIPMENT?          Yes.

IS RESCUE PLAN IN PLACE?          Yes	     No	        Why Not?	
                                                                                        .No.
HAS THE AREA BEEN SECURED AND ALL ENERGY SOURCES LOCKED AND TAGGED?          Yes_

IS THERE ANY POSSIBILITY OF ENTRAPMENT OR ENGULFMENT BY PART1CULATE MATTER?    Yes.

IF YES. HAS PROVISION BEEN MADE TO WORK SAFELY IN SUCH AN AREA'.1      Yes	No_

ARE O2, LEL AND TOXIC SUBSTANCES WITHIN SAFE LIMITS?                 Yes	No_
                                                                                        .No.

                                                                                         No
/ certify that the above conditions are accurate and validate the entry only for the stated purpose, time and identified employees, not to
exceed work for one shift.  If any question was answered "NO" and a satisfactory answer was not given DO NO ISSUE PERMIT.

Date:	

Signature:	

Title:

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                             SECTION: APPENDIX E
                             VERSION: REVISION/MAY 1997
ATTACHMENT III

    References

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                                                              SECTION: APPENDIX E - ATTACHMENT III
                                                              VERSION: REVISION/MAY 1997
                                        REFERENCES


Safety  Requirements  for Working in Tanks and Other Confined Spaces, American  National Standards
Institute, Zl 17.1.1977

Safety Requirements for Confined Spaces, American National Standards Institute, Zl 17.1.1989

Criteria for a Recommended Standard.  Working in Confined Space,  National Institute  for Occupational
Safety and Health, December, 1979

Permit Required Confined Spaces, Federal Register, 29 CFR 1910.146, Vol 54, No. 10

Safe Vessel Specification Manual, U.S. EPA, 1977

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                                                           SECTION:  APPENDIX F
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE: 1 of 2
APPENDIX F: HOT WORK PROCEDURES
Hot Work Permitting System

Hot work is defined as the cutting, shaping or joining of metals by use of heat produced by flame
or electric arc.   This includes welding, brazing, soldering and the  use of cutting torches, blow
torches and similar activities.  In  addition, the loosening of frozen nuts  or  bolts or even paint
removal can also be classified as hot work.

Hot work activities conducted by  EPA or contractor personnel will be done through a permit
system.  The permit  is an authorization, in writing,  for the worker to proceed with the hot work
activities.  The  hot work permit  will list the  time,  place,  type of work, and  certify  that hazards
have been evaluated and proper protective equipment has been authorized.  See Attachment 1 of
this Appendix for  a sample of a Hot Work Permit.   The following is a minimum  list of items that
should be provided  on the permit:

1)     Date, shift,  and location of work being performed.

2)     Description of work being performed.

3)     Potential hazards that the  worker may  encounter (e.g.,  surrounding areas such as adjoining
       tanks/vessels)

4)     Isolate mechanical and electrical systems.

Authorized personnel will evaluate hot work operations  based  on  the Hazard  Communication
Program  stated in CFR 1910.1200.  Chemical  inventory lists and Material Safety Data Sheets will
be referenced  prior  to  hot  work  to  identify the   location  and reactive  characteristics  of any
dangerous or potentially  dangerous materials  where the hot  work will  occur.   This will  allow
personnel to  monitor for  chemical  and  physical agents before and during hot work activities.  It
will ensure  that the proper  engineering  controls have  been  implemented and that appropriate
personal protective  equipment is being used as  well.

Implementation  of  Engineering Controls:  In  some situations it may be  necessary to ventilate,
isolate or implement some other control  to reduce the hazard of the task performed.

Wearing  of Proper  Personal Protection Equipment:  After the initial air monitoring and evaluation
of the hot work site is performed, proper  protective  equipment  will be  selected.   Personal
protective equipment may include gloves, coveralls, boots,  aprons, head cover, ear protection, eye
protection, face protection, and/or respiratory protection.

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                                                          SECTION: APPENDIX F
                                                          VERStON: FINAL/MAY 1997
                                                          PAGE:  2 of 2
WORK PRACTICES

Fire Watch

Suitable fire extinguishing equipment will  be maintained  and stationed at the work  site.   Any
welding or cutting activity will require a fire watch person to observe the individual performing
the hot  work.  Cutting or welding on  a partition (wall, deck, etc.) may require a second fire watch
in order to observe the other side of the wall, deck, etc.  The  person will be trained in use of the
fire  extinguishing devices and trained in alerting the  proper  authorities in case of an accident.
After completion of hot work, a fire watch will be kept for half an hour.

Posting the Permit and Any Necessary Warning Signs

Depending on  the  work activity  and the  hazards involved,  signs will  be posted to offer an
important reminder to the individual  conducting the work and also warn others outside the work
area of the hazards associated with the task being performed.

Keeping the Work Area Free of Hazards

The work area will  be inspected for  flammable  debris and other hazards that  may contribute to
injury or death.  Flammable items will be removed or isolated  from the work area.  If it is not
possible to remove the hazard it may be  necessary to move the  work area to a new location.  If
there is no safe way of conducting the hot work activity it will be necessary to pursue alternative
methods.

Additionally, all  gas cylinder  regulators  must be closed at  the end of use.  Acetylene and oxygen
hoses must be rolled-up at the end of each use and stored in such a way that they will not rupture,
present a fire hazard or a confined space hazard.

Hot Work Permits

The permit should only be posted at the work area and  allowed  for one shift.   If work  is to
continue during  another shift or day, a  new  permit must be issued  before hot  work activities
resume.

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                                                  SECTION:  APPENDIX F, ATTACHMENT 1
                                                  VERSION:  FINAL/MAY 1997
                                                  PAGE: 1 of 1
Attachment 1 - FIRE PROTECTION - HOT WORK PERMIT

In an effort to  establish control  over operations using  open flames or producing sparks (e.g.
welding, cutting, burning),  authorization  must be secured before equipment capable of igniting
combustible materials is used outside areas normally specified.

Fill out  form prior to conducting any hot work.  After conducting hot work, the Hot Work Permit
should be returned to the maintenance department which  will collect and forward it to the Safety
Department for filing and record purposes.

1.     Area where work is to be done (including surrounding area) has been inspected and is free
       of any unnecessary combustible or flammable materials:
       Yes  	         No  	

2.     The designated  fire watch is:  	,
                                           (Name of Person)

3.      Fire extinguishing equipment is available in the hot work area:
       Yes 	         No 	

4.      All departments and/or personnel concerned have been notified of the Hot Work activities:
         Yes 	       No 	

5.      Combustible materials have been isolated from sources of ignition in the hot work area:
       Yes 	         No 	

6.      Only trained and authorized personnel are allowed to conduct hot work in the work area:
        Yes  	         No 	

1.      Date of issue:    	
       Time permit is valid:
9.      Name of person completing form:
10.    Name of Supervisor authorizing hot work to be performed:
Comments:

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                                                          SECTION:  APPENDIX G
                                                          VERSION:  FINAL/MAY 1997
                                                          PAGE:  1 of 5
APPENDIX G: LOCKOUT/TAGOUT PROGRAM

INTRODUCTION

This Lockout/Tr gout Program  has been developed to comply with the Occupational Safety and Health
Administration's (OSHA)  Control of Hazardous Energy Sources Standard in 29 CFR 1910.147. This
program  will  safeguard the  health and well-being  of GLNPO personnel exposed  to  the  hazards
presented  by  energized machines or equipment. Rigorous compliance with  this program will ensure
that  machines and equipment  are isolated from all potentially hazardous energy sources, locked out
and  tagged out, before any employees perform any  servicing or  maintenance activities where the
unexpected energization,  start-up or release of stored energy could cause serious injury or death.
Energy  sources present  in  the facility  includes electrical,  mechanical, hydraulic,  and pneumatic
sources.
The  Captain has been appointed to administer the  Lockout/Tagout Program, and has full support to
effectively  manage this program, assisted by the electrical engineers  and marine technicians.   The
administrator has been authorized to carry out this program without delay.
PROGRAM OF COMPLIANCE

The Lockout/Tagout Program will include the following components:

       •  Energy Source Surveys
       •  Authorized Employees
       •  Affected Employees
       •  Training
       •  Lockout/Tagout Methods
       •  Lockout/Tagout Procedures
       •  Restoring Equipment to Use
       •  Outside Contractor Information Exchange


ENERGY SOURCE  SURVEYS

It  is the intent of GLNPO to conduct a  detailed energy source survey of all equipment and machines
in  the facility. The initial survey will include the identification of all energy sources for each piece of
equipment or machinery and  location of energy shutoffs for each piece of equipment. This survey will
result in the production of a written  lockout/tagout control procedure for each piece of equipment.
New  equipment or  machinery will be  inspected during installation and  a written lockout/tagout
procedure will be developed for each piece of new equipment before it is energized.

Follow-up surveys will  be made yearly  and  at such a time as engineering or  production procedures
result in a changes to energy sources or controls.

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                                                          SECTION: APPENDIX G
                                                          VERSION: FINAL/MAY 1997
                                                          PAGE  2 of 5
AUTHORIZED EMPLOYEES

Lockout/tagout authorized associates are persons who have been trained  in the procedures necessary to
lockout and tagout equipment or machinery prior to servicing or performing maintenance on it.  Only
authorized associates may install or remove locks and tags installed as part of this program.
AFFECTED EMPLOYEES

Affected employees  include any associate who operates  or  uses machinery or equipment on which
servicing or maintenance is being performed under lockout/tagout procedures, or who has to work  in
an area in which servicing or maintenance work is being performed. All associates in the mechanical
areas  of GLNPO operational areas are considered to be affected employees and will  be trained  to
recognize locks and tags used for lockout/tagout and the need for lockout/tagout procedures.

Any associate who  attempts to  remove, alter, bypass, or  override a lockout/tagout will be subject  to
disciplinary action.
TRAINING

Training will be provided to  all authorized associates in the procedures for lockout/tagout.  Only
trained associates will be authorized to initiate or remove lockout/tagouts.

All affected associates will receive lockout/tagout awareness training.

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                                                           SECTION:  APPENDIX G
                                                           VERSION:  FINAL/MAY  1997
                                                           PAGE:  3 of 5
LOCKOUT/TAGOUT METHODS

Lockout/tagout will  be accomplished using a padlock or combination of padlock and  multiple lock
hasp in combination with a warning tag. The  padlock  will ensure that the hazardous energy source
cannot be  re-energized.  Chains and  special  hardware may also be used to  isolate energy sources.
Padlocks with keys will be available at Lockout/Tagout stations,  with a master key retained in the key
safe.

The warning tag will communicate the reasons why the energy source should not be re-energized. The
warning  tag will  be attached  at the lockout and  at the  equipment control  panel, and will  be of
non-reusable, all-weather construction, fastened with a nylon tie.  The name of the authorized associate,
date  and time  ^f  the  lockout/tagout, and  a  brief description  of the work to be  performed will  be
included on warning  tag.
LOCKOUT/TAGOUT PROCEDURES

Prior to initiating any repairs, modifications and/or adjustments to operating equipment or machines,
the following steps will be followed:
        1.      The immediate manager for the affected equipment or machines will be notified
               that the energy sources will be deactivated using the Lockout/Tagout procedures.

        2.      All  sources of  energy  to  the  affected equipment will  be  identified by  the
               authorized associate.  Energy sources  include mechanical,  electrical, hydraulic  and
               pneumatic.

        3.      All  controls will be turned off, and all  energy to the affected  equipment will be
               turned off or blanked and locked out. Stored energy will be released. If more than
               one authorized employee is assigned to work on  the equipment, a multiple lock
               hasp will be used.

        4.      A warning  tag will be filled out and affixed to the controls and to the lockout
               device(s).

        5.      Prior to starting  any  work on the locked out equipment, the authorized employee
               will attempt to  start the equipment  to  ensure  that the lockout device provides
               adequate  protection. Operating controls  must be reset to the "off position after
               this test.

        6.      Work  on the  locked out/tagged  out equipment  may  now be performed, with
               caution and continuous observations for hidden energy sources.

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                                                          SECTION: APPENDIX G
                                                          VERSION: FINAL/MAY 1997
                                                          PAGE:  4 of 5
RESTORING EQUIPMENT TO USE

After the completion of all  repairs,  maintenance  and/or  adjustments to the locked  out/tagged out
equipment, it will be restored to use by following these steps:
        1.      The authorized associate and the line manager will verify that the area around the
               equipment is safe to reenergize. No associates or tools will be permitted in unsafe
               areas  around  the  equipment, and all  guards  and  covers  will  be  properly
               reconnected.

       2.      Lockout/tagouts will  be removed by the authorized  associate.  If work will  be
               carried out over several shifts, the outgoing authorized associate will return the
               key to the  Administrator or Assistant Administrator for safekeeping in  a secure
               location. All locked out equipment will  be  tested to  ensure that it remains in a
               de-energized state.

       3.      If the work is completed, the lock shall not be removed from the equipment until
               the  authorized  employee removes it,  or until  the  lockout/tagout administrator
               investigates the equipment, verifies the safety of the equipment,  and verifies that
               the authorized employee is unavailable to remove his lock and  attempts to contact
               him.  The authorized associate will be notified of the removal of  his lock  when he
               returns to the building.

       4.      The equipment is restarted.
OUTSIDE CONTRACTOR INFORMATION EXCHANGE

Whenever outside contractor personnel are engaged to perform work on equipment or machines, the
Lockout/Tagout administrator will provide a copy of the GLNPO Lockout/Tagout Program and receive
a copy of the contractor's written program. All authorized and affected associates will be informed of
significant  differences  between  the  two programs and  will observe  the  locks and tags  of both
programs.

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                                                          SECTION:  APPENDIX G
                                                          VERSION:  FINAL/MAY 1997
                                                          PAGE:  5 of 5
LOCKOUT PROCEDURE CHECKLIST


1.      Train authorized lockout/tagout associates and affected associates.

2.      Assign work and define work area.

3.      Identify all energy sources to equipment.

4.      Notify affected associates.

5.      Turn off energy sources at controls, if possible.

6.      Turn off energy sources at breakers or valves, releasing stored energy.

7.      Attempt to restart equipment.

8.      Check for operation.

9.      Return controls to "off' position.

10.    Install warning tags at all  locks and controls.

11.    Perform  repair, maintenance, servicing or adjustment.

12.    Check equipment and notify affected associates.

13.    Remove warning tags and lockouts.

14.    Restore equipment to operating conditions.

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                                                   SECTION: APPENDIX H
                                                   VERSION: FINAL/MAY 1997
                                                   PAGE: 1 of 32
APPENDIX H: BLOODBORNE PATHOGENS PROGRAM - R/V LAKE GUARDIAN
TABLE OF CONTENTS

SECTION I:

SECTION II:
SECTION III:

SECTION IV:
SECTION V:
SECTION VI:

SECTION VII:
SECTION VIII:
PURPOSE OF THE PLAN

GENERAL PROGRAM MANAGEMENT

A. Responsible Persons
B. Availability to Employees
C. Review and Update

EXPOSURE DETERMINATION

METHODS OF COMPLIANCE

A. General
B. Engineering Controls
C. Work Practice Controls
D. Personal Protective Equipment
E. Housekeeping

HEPATITIS B VACCINATION, POST-EXPOSURE EVALUATION
AND FOLLOW-UP

A. Flepatitis B Vaccination
B. Post-Exposure Evaluation and Follow-Up
C. Information Provided to the health care Professional
D. health care Professional's Written Opinion
E. Medical Recordkeeping

LABE-LS AND SIGNS

INFORMATION  AND TRAINING

A. Training Topics
B. Training Methods
C. Recordkeeping

HIV AND HBV RESEARCH LABORATORIES AND
PRODUCTION FACILITIES

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                                                         SECTION: APPENDIX H
                                                         VERSION: FINAL/MAY 1997
                                                         PAGE: 2 of 32
SECTION I

PURPOSE OF THE PLAN

One  of the major goals of the Occupational  Safety and  Health  Administration (OSHA) is  to
regulate facilities where work  is carried  out  to  promote safe work  practices  in  an effort  to
minimize the incidence  of illness and injury experienced by employees.  Relative  to  this goal,
OSHA has enacted the  Bloodborne Pathogens Standard,  codified as 29 CFR  1910.1030.  The
purpose of the Bloodborne Pathogens Standard  is to "reduce occupational exposure to Hepatitis B
Virus (HBV),  Human  Immunodeficiency Virus  (HIV)  and  other  bloodborne  pathogens  that
employees may encounter in their workplace.

The US EPA Great Lakes National Program Office (GLNPO) believes that there  are a number  of
"good  general  principles that  should  be followed when  working with  bloodborne pathogens."
These include:

•  It is prudent to minimize all exposure to bloodborne pathogens.

•  Risk of exposure to bloodborne pathogens should never be underestimated.

•  Our facility should  institute as many work practices  and engineering controls a possible  to
   eliminate or minimize employee exposure to bloodborne pathogens.
We have  implemented this Exposure  Control Plan to meet the letter and intent of the OSHA
Bloodborne Pathogens Standard.  The objective of this plan is two fold:

•   To protect our employees from the health hazards associated with bloodborne pathogens.

•   To  provide   appropriate  treatment  and counseling  should  an  employee  be exposed to
    bloodborne pathogens.

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                                                          SECTION:  APPENDIX H
                                                          VERSION:  FINAL/MAY 1997
                                                          PAGE:  3 of 32
SECTION II

GENERAL PROGRAM MANAGEMENT

A. RESPONSIBLE PERSONS

There are four major "Categories of Responsibility" that are central to the effective implementation
of our Exposure Control Plan. These are:

• The "Exposure Control Officer"

• Department Managers and Supervisors

• Education/Training Instructors

• Our Employees

The  following sections define the roles played  by each of these groups in carrying out the plan.
(Throughout this written plan, employees with  specific responsibilities are identified.  If, because
of promotion  or other reasons  a new employee is  assigned any  of these  responsibilities,  the
GLNPO  Safety Manager and/or the Ship Operations Project Officer  is to be notified of the change,
so that they can  update their records.)


EXPOSURE CONTROL OFFICER

The  "Exposure Control Officer" will  be responsible  for overall  management and support of  our
facility's  Bloodborne  Pathogens  Compliance  Program.    Activities which  are delegated to  the
Exposure Control Officer typically include but are not  limited to:

•   Overall responsibility for implementing the Exposure Control  Plan for  the entire facility.

    Working with management  and other  employees to develop and  administer  any additional
    bloodborne  pathogens-related  policies and  practices  needed  to  support  the  effective
    implementation of this plan.

•   Looking for ways to improve an  Exposure Control Plan, as  well as to  revise and update the
    plan  when necessary.

•   Collecting and maintaining a suitable reference library on the Bloodborne Pathogens Standard
    and bloodborne pathogens safety and health  information.

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                                                          SECTION:  APPENDIX H
                                                          VERSION:  FINAL/MAY 1997
                                                          PAGE: 4 of 32
    Knowing current legal requirements concerning bloodborne pathogens.

    Acting as the facility liaison during OSHA inspections.

    Conducting periodic facility audits to maintain an up-to-date Exposure Control Plan.
A  designated  EPA employee  or contractor has been appointed the facility's Exposure Control
Officer.

We have determined that the Exposure Control Officer will require  assistance in fulfilling his/her
duties.   To  assist them  in  carrying out  their duties, we have  created  an  Exposure Control
Committee composed of the following people.  (If no committee is to be established, write ''No
committee established").

                                Exposure Control Committee
      Chemical Hvaiene Officer - Co-Chair
      US EPA's Chief Scientist - Co-Chair
      Analytical Contract Project Representative
       Ship Operating Contract Representative
       Additional Representatives from Ship Safety Committee (as needed)
DEPARTMENT MANAGER AND SUPERVISORS

Department Managers and Supervisors are  responsible for  exposure control  in  their respective
areas.  They work  directly with the Exposure Control Officer  and our employees to ensure that
proper exposure control procedures are followed.

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                                                           SECTION:  APPENDIX H
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE:  5 of 32
EDUCATION/TRAINING COORDINATOR

Our  Education/Training Coordinator will responsible for providing information and training to all
employees  who nave the potential for exposure to bloodborne pathogens.  Activities falling under
the direction of the Coordinator include:

•   Maintaining an up-to-date  list of facility  personnel requiring  training (in  conjunction with
    facility management).

•   Developing suitable education/training programs.

•   Scheduling periodic training seminars for employees.

•   Maintaining appropriate  training documentation  such  as Sign-In Sheets,  Quizzes,  Training
    Agenda, etc.

•   Periodically reviewing  the training programs with the Exposure Control   Officer, Department
    Managers and  Supervisors to  include appropriate new information.

GLNPO's Safety Manager  has been selected to be the  facility's Education/Training Coordinator.

EMPLOYEES

As with all  of our  facility's  activities,  our  employees have  the  most  important  role  in  our
Bloodborne Pathogens Compliance Program, for the ultimate execution of much of our Exposure
Control Plan rests  in their hands.  In this role, they must do such things as:

•   Know what tasks they perform that have the potential for occupational  exposure.

•   Attend the bloodborne  pathogens training sessions.

•   Plan and conduct  all operations in accordance with our work practice controls.

    Develop good  personal hygiene habits.


B. AVAILABILITY OF THE EXPOSURE CONTROL PLAN TO EMPLOYEES

To help them  with their efforts, our facility's Exposure  Control Plan is available to  our employees
at any time.  Employees are advised of this availability during their education/training sessions.
Copies of the Exposure Control Plan are kept at the following locations:

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                                                        SECTION:  APPENDIX H
                                                        VERSION:  FINAL/MAY 1997
                                                        PAGE:  6 of 32
•   Chemical Hygiene Officer's Office

•   GLNPO Headquarters

•   Designated First Aid Area

•   Contained within GLNPO's Safety Health and Environmental Compliance Manual


C. REVIEW AND UPDATE OF THE PLAN

We recognize that it is important to keep our Exposure Control Plan up to date.  To ensure this,
the plan will be reviewed and updated under the following circumstances:

•   Annually, on or  before May 5 of each year.

•   Whenever new or modified tasks and  procedures are implemented which affect occupational
    exposure of our  employees.

    Whenever our employees' jobs  are  revised in such ways that new occupational exposure may
    occur.

•   Whenever we establish new functional  positions within our facility that may involve exposure
    to bloodborne pathogens.

SECTION III

EXPOSURE DETERMINATION

One of the keys to implementing  a successful Exposure Control Plan  is to  identify  exposure
situations employees may encounter.  To  facilitate  this in  our facility,  we have prepared the
following lists:

•   Job  classifications  in  which  all  employees have  occupational  exposure  to bloodborne
    pathogens.

•   Job  classifications in  which some employees have  occupational  exposure to bloodborne
    pathogens.

    Tasks and procedures  in which occupational exposure to bloodborne pathogens occurs  (these
    tasks and procedures are performed by employees in the job classifications shown on the two
    previous lists).

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                                                       SECTION:  APPENDIX H
                                                       VERSION:  FINAL/MAY 1997
                                                       PAGE:  7 of 32
GLNPO Safety Manager and  the Ship Operations  Project  Officer will  work with department
managers  and supervisors  to revise  and  update  these lists as  our  tasks, procedures, and
classifications change.

JOB  CLASSIFICATIONS  IN  WHICH  ALL  EMPLOYEES HAVE  EXPOSURE TO
BLOODBORNE PATHOGENS

Below are listed the job classifications in our facility where all employees may come into contact
with human blood or other  potentially infectious materials, which may result in possible exposure
to bloodborne pathogens:

JOB TITLE                              DEPARTMENT/LOCATION

Ship's Chemical Hygiene Officer            R/V Lake Guardian

Chief Scientist                           R/V Lake Guardian

Ship's Operations Contractor Designee       R/V Lake Guardian
JOB  CLASSIFICATIONS  IN WHICH  SOME EMPLOYEES  HAVE EXPOSURE TO
BLOODBORNE PATHOGENS

Below are listed the job classifications in our facility where some  employees  may come into
contact with  human blood or other potentially infectious materials, which may result in possible
exposure to bloodborne pathogens:

JOB TITLE                              DEPARTMENT/LOCATION

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                                                         SECTION:  APPENDIX H
                                                         VERSION:  FINAL/MAY 1997
                                                         PAGE:  8 of 32
WORK ACTIVITIES INVOLVING POTENTIAL EXPOSURE TO BLOODBORNE
PATHOGENS

Below are listeo the tasks and procedures in our facility where employees may come into contact
with human blood  or other potentially infectious  materials  which may result  in  exposure  to
bloodborne pathogens.
TASK                     JOB CLASSIFICATION       DEPARTMENT LOCATION

First Aid/CPR Responder    Chief Scientist
                           Ship's Chemical Hygiene Officer
                           Ship's Operations Contractor Designee
                           Ship's Analytical Contractor or Designee
SECTION IV
METHODS OF COMPLIANCE

We understand that there are a number of areas that must be addressed in  order to effectively
eliminate or minimize exposure to bloodborne pathogens  in our facility.  The first five areas we
deal with in our plan are:

•   The use of Universal Precautions.

•   Establishing appropriate engineering controls.

•   Implementing appropriate Work Practice Controls.

•   Using necessary Personal Protective Equipment.

•   Implementing appropriate housekeeping procedures.

Each  of these areas are reviewed with our employees during their bloodborne pathogens  training
(see the "Information and Training" section of this plan for additional information).  By rigorously
following the requirements of OSHA's Bloodborne Pathogens Standard in these five areas, we feel
that we will eliminate or minimize our employees' occupational exposure to bloodborne pathogens
as much as possible.

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                                                          SECTION:  APPENDIX H
                                                          VERSION:  FINAL/MAY 1997
                                                          PAGE:  9 of 32
A. UNIVERSAL PRECAUTIONS

We began the use of Universal  Precautions on September 1, 1993.  As a result, we treat all human
blood and potentially infectious body fluids  (excluding saliva not containing visible blood) as if
they are known to be infected with HBV, HIV and other bloodborne pathogens.

In circumstances where it is difficult or impossible to differentiate  between body fluid types, we
assume all body fluids to be potentially infectious.

The  Designated Exposure Control  Officer is  responsible for overseeing our Universal Precautions
Program.
B. ENGINEERING CONTROLS

One of the key  aspects to our Exposure Control Plan is  the  use of Engineering Controls to
eliminate or minimize employee exposure to bloodborne pathogens.  As a result,  employees use
cleaning, maintenance and other  equipment that  is designated to prevent contact  with blood or
other potentially infectious materials.

The Designated  Exposure  Control Officer  periodically works  with department  managers  and
supervisors to review tasks and procedures performed  in our facility where engineering controls
can be  implemented  or updated.   As a part of  this  effort, a facility survey was completed in
October 1992 identifying three things:

•   Operations where engineering  controls are currently employed.

    Operations where engineering  controls can be updated.

•   Operations currently not employing engineering controls,  but where engineering controls could
    be beneficial.

The results of this survey can be found on the following pages.

Each  of  these  lists is  reexamined  during our annual Exposure  Control  Plan  review  and
opportunities for  new or improved engineering  controls are  identified. Any existing engineering
control  equipment is also reviewed for  proper function  and needed  repair  or replacement each
month, in  conjunction with  the department manager or supervisor  where the equipment is located.

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                                                         SECTION:  APPENDIX H
                                                         VERSION:  FINAL/MAY 1997
                                                         PAGE:  10 of 32
ENGINEERING CONTROL EQUIPMENT

The  following operations  have, or should have,  Engineering Control Equipment to eliminate or
minimize our employees'  exposure to bloodborne pathogens. If equipment  is needed but not yet
available "None" is indicated in the "Control Equipment" column.
DEPARTMENT/
OPERATION
CONTROL
EQUIPMENT
NEEDS
UPDATING
DATE OF LAST
REVIEW
Analytical Laboratory Safety Shower Monthly Monthly
Eye Wash
      Throughout
 Designated First Aid
      Station  	
Sinks, spigots
 Waste Receptacles
Routine
                                                           Maintenance
 Monthly
Routine
                                               Maintenance
Monthly

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                                                           SECTION:  APPENDIX H
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE:  11 of 32
In addition to the engineering controls identified on these lists, the following engineering controls
are used throughout the facility:

•       Handwashing facilities (or antiseptic hand cleaners and towels or antiseptic towelettes), are
        readily accessible to all employees who have the potential for exposure.

        Self-sheathing needles. (MEDICAL).

•       Containers for contaminated reusable sharps having the following characteristics:
               - Puncture resistant.
               - Color-coded or labeled with a biohazard warning label.
               - Leak-proof on the sides and bottom.
        (MEDICAL)

•       Specimen containers which are:
               - Leak proof.
               - Color-coded or labeled with a biohazard warning label.
               - Puncture-resistant if necessary.
        (MEDICAL)

•       Secondary containers which  are:
               - Leak-proof.
               - Color-coded or labeled with a biohazard warning label.
               - Puncture-resistant, if necessary.
        (MEDICAL)
C. WORK PRACTICE CONTROLS

In addition to engineering controls, our facility uses a number of Work Practice Controls to help
eliminate  or minimize  employee  exposure  to bloodborne  pathogens.    Many of  these  Work
Practiced Controls have been in effect for some time.

The  person  in  our facility who is  responsible  for overseeing the implementation of these  Work
Practice Controls is the Designated Exposure Control Officer.  He/she works  in conjunction with
department  mangers,   supervisors  and  our  facility's  training  coordinators  to  effect   this
implementation.

Our  facility  has  adopted  the following Work  Practice  Controls  as part of  our  Bloodborne
Pathogens Compliance Program:

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                                                           SECTION:  APPENDIX H
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE:  12  of 32
•  Employees wash  their hands immediately, or as soon as feasible, after  removal of potentially
   contaminated gloves or other personal protective equipment.

•  Following any  contact of body  areas with blood or any other potentially infectious materials,
   employees wash their hands and any other exposed skin with  soap and water as soon a possible.
   They also flush exposed mucous membranes with water.

•  Contaminated needles and other  contaminated sharps are not bent, recapped or removed unless:
               It can be demonstrated that there is no feasible alternative.
               The  action required is by specific medical procedure.
               In  the  above situations the recapping or needle removal is accomplished through
               the use of a  medical device or a one-handed technique.
   (MEDICAL)

•  Contaminated reusable sharps are  placed  in appropriate  containers  immediately, or as soon as
   possible after use.
   (MEDICAL)

•  Eating, drinking,  smoking, chewing gun,  applying cosmetics or lip balm, and handling contact
   lenses  is  prohibited  in  work  areas where there  is  potential  for exposure  to bloodborne
   pathogens.

•  Food  and drink is  not kept in refrigerators,  freezers, on  counterlops or in other  storage areas
   where blood or other potentially infectious materials  are present.

•  Mouth pipetting/suctioning of blood or other infectious materials is prohibited.
   (MEDICAL)

•  All procedures involving blood or other  infectious materials minimize  splashing, spraying or
   other actions generating droplets of these materials.

•  Contaminated  or  potentially  contaminated materials  are  placed   in  designated leak-proof
   containers, appropriately  labeled, for handling and storage.

•  If outside contamination of a primary container of  infectious material occurs, that container is
   placed within a second leak-proof container, appropriately labeled, for handling and storage.  (If
   infectious  material  can  puncture  the  primary  container,  the  secondary  container  must  be
   puncture resistant.)

 Equipment  which  becomes  contaminated  is  examined  prior  to servicing  or  shipping,  and
 decontaminated as necessary unless it can be  demonstrated that decontamination  is not feasible.

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                                                           SECTION:  APPENDIX H
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE:  13 of 32
               An   appropriate  biohazard   warning  label  is  attached  to  any  contaminated
               equipment, identifying the contaminated portions.

               Information regarding the remaining contamination  is conveyed to all  affected
               employees, the equipment manufacturer and  the  equipment service representative
               prior to handling, servicing or shipping.

When a new employee comes to our  facility, or an employee changes jobs within the facility, the
following process occurs to ensure that they are trained in the appropriate work practice controls:

   •    The employee's job  classification and the tasks  and  procedures  they  will perform  are
       checked against  the Job  Classifications and Tasks List which we have identified in  our
       Exposure Control Plan as those in which the potential for occupational exposure exits.

   •    If the  employee  is transferring from  one job to  another  within our  facility,  the  job
       classification and tasks/procedures  pertaining  to their previous  position are  also  checked
       against these lists.

       Based on this  "cross checking",  the new job classifications and/or tasks and  procedures
       which will bring the employee into occupational exposure  situations are identified.

   •    The employee  is then trained by the facility  Training  Coordinator or another  instructor
       regarding any work practice controls that the employee is not experienced with.
D. PERSONAL PROTECTIVE EQUIPMENT

Personal  Protective Equipment  is our employees' last line of defense against bloodborne pathogen
exposure.  Because of this, our facility  provides  for  our employees, at no  cost to them,  the
Personal  Protective Equipment  that they need to protect themselves against such exposure.  This
equipment includes but is not limited to:

• Gloves
• Safety glasses
• Goggles
• Face shields/masks
• Respirators
• Protective Clothing

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                                                           SECTION: APPENDIX H
                                                           VERSION: FINAL/MAY 1997
                                                           PAGE:  14 of 32
Hypoallergcnic  gloves,  glove  liners and similar alternatives are  readily  available  to those
employees who are allergic to the gloves our facility normally uses.

The Designated Exposure  Control Officer, working with  department managers and  supervisors,  is
responsible for ensuring that all  departments and work areas have appropriate personal protective
equipment available to all employees.

Our employees are trained regarding the use of appropriate personal protective equipment for their
job classifications and task/procedures they perform.  Training is  provided  when necessary if an
employee takes a new position or news job functions are added to their current position.

To determine the need for additional training, the employee's previous job classification and tasks
are compared to those for any  new job  or function.   Any  needed training is provided by  their
department manager or supervisor working with our facility's Training Coordinator.

To ensure that all personal  protective equipment  is not contaminated and  is in the appropriate
condition to protect employees  from potential exposure, our facility adheres  to  the  following
practices:

   •    All personal protective  equipment  is inspected periodically and repaired  or replaced as
        needed to maintain its effectiveness.

   •    Reusable personal protective  equipment  is  cleaned,  laundered  and decontaminated  as
        needed.

        Single-use  personal protective equipment, or equipment that cannot,  for whatever  reason,
        be  decontaminated,  is  disposed  of by  forwarding  that  equipment to the  Designated
        Exposure Control Officer.

To make sure that this equipment is used  as effectively  as possible, our employees are to adhere to
the following practices when using their personal  protective equipment:

   •    Any garments penetrated by blood or other potentially infectious materials are removed
        immediately or as  soon as feasible.

   •    All potentially contaminated  personal protective equipment is removed prior to leaving a
        work area.

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                                                           SECTION: APPENDIX H
                                                           VERSION: FINAL/MAY 1997
                                                           PAGE:  15 of 32
       Gloves are worn in the following circumstances:
       -  Whenever  the employees anticipate  hand contact  with blood or  other  potentially
       infectious materials.
       - When handling or touching contaminated items or surfaces.

   •    Disposable gloves are replaced as soon as practical after contamination or if they are torn,
       punctured or otherwise lose their ability to function as an exposure barrier.

   •    Utility gloves are decontaminated for reuse unless they are cracked,  peeling, torn or exhibit
       other signs of deterioration at which time they are disposed of.

   •    Masks and eye  protection (such as goggles,  face shields, etc.) are used whenever  splashes
       or sprays may generate droplets of infectious materials.

   •    Protective  clothing (such as coats) are worn whenever  potential exposure to the  body is
       anticipated.
E. HOUSEKEEPING

Maintaining our facility  in a clean and sanitary condition is an important part of our Bloodborne
Pathogens Compliance Program.  To  facilitate this, we have set up a written schedule for cleaning
and decontamination of the appropriate areas of the facility.  The schedule provides the following
information:

   • The area to be cleaned/decontaminated.

   • Day and time of scheduled work.

   • Cleansers and disinfectants to be  used.

   • Any special instructions that are appropriate.

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                                                            SECTION:  APPENDIX H
                                                            VERSION:  FINAL/MAY 1997
                                                            PAGE:  16 of 32
Using this schedule, our janitorial/cleaning staff employs the following practices:

•  All equipment and surfaces are cleaned and decontaminated after contact with
   blood or othc, potentially infectious materials:

               - After the completion of medical procedures.  (MEDICAL)
               - Immediately (or as soon as feasible) when surfaces are overtly  contaminated.
               - After any spill of blood or infectious materials.
               - At the end of the work shift if the surface may  have been contaminated during
                 that shift.

•  Protective coverings (such as plastic trash bags or wrap, aluminum foil or absorbent paper) are
   removed and replaced:

               - As soon as it is feasible when overtly contaminated.
               - At the end of the work  shift  if they  may have been contaminated during the
                 shift.

•  All trash containers, pails, bins and other receptacles  intended for use routinely are inspected,
   cleaned and decontaminated as soon as possible if visibly contaminated.

•  Potentially contaminated  broken  glassware  is picked  up  using mechanical means (such  as a
   dustpan  and brush, tongs, forceps, etc.).

•  Contaminated reusable  sharps are stored in  containers that do  not  require "hand processing".
   (MEDICAL)

The  Designated Exposure  Control  Officer is  responsible for  setting  up  our cleaning  and
decontamination schedule making sure it is carried out within our facility.

We are  also very careful in our facility in handling regulated waste (including bandages, feminine
hygiene products and other potential infectious materials).

•  They  are discarded or "bagged"' in containers that are:

               - Able to be closed.
               - Puncture-resistant if the discarded materials have the potential to
                 penetrate the container.
               - Leak-proof if the potential for fluid spill or  leakage exists.
               - Red in color or labeled  with the appropriate biohazard warning label.

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                                                            SECTION:  APPENDIX H
                                                            VERSION:  FINAL/MAY 1997
                                                            PAGE:  17 of 32
•  Containers for this regulated waste are placed in appropriate locations  in our facility within easy
   access of our employees and as close as possible to the sources of the waste.

•  Waste containers are maintained upright, routinely replaced and not allowed to overfill.

•  Contaminated laundry is  handled as little as possible  and  is  not  sorted or rinsed where it is
   used.

•  Whenever  our  employees move containers  of regulated waste from  one  area to another, the
   containers  are  immediately closed  and placed  inside  an  appropriate  secondary container if
   leakage is possible from the first container.

The  Designated Exposure Control  Officer is responsible for  the collection  and handling of our
facility's contaminated waste.
CLEANING SCHEDULE

SCHEDULED   CLEANERS/
EQUIPMENT/   CLEANING
AREA

    First Aid Station
DISINFECTANTS
(DAYAITME)

  After Use	
SPECIAL
USED
                        INSTRUCTIONS
   Solutions with
                                                    bleach or NaCl 04

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                                                          SECTION:  APPENDIX H
                                                          VERSION:  FINAL/MAY 1997
                                                          PAGE: 18 of 32
SECTION V

HEPATITIS B VACCINATION, POST-EXPOSURE EVALUATION AND FOLLOW-UP

Everyone  in our facility recognizes that even with proper adherence to all our exposure prevention
practices,  exposure  incidents  can occur.    As  a  result,  we  have  implemented a  Hepatitis B
Vaccination Program as well  as set up  procedures  for post-exposure  evaluation and  follow-up
should exposure to bloodborne pathogens occur.

NOTE:       Contract  personnel  are  responsible for  implementing  their own  post-exposure
             evaluation and follow-up plan that at least meets the requirements of this section.


A. VACCINATION PROGRAM

To  protect our employees as much as possible from  the possibility of  Hepatitis  B infection, our
facility  has implemented a  vaccination  program   This program  is available,  at  no cost, to all
employees who have occupational exposure to bloodborne pathogens.

The vaccination program consists of a series of three inoculations over a  six-month period.  As
part of their bloodborne pathogens training, our employees  have received information  regarding
the Hepatitis B vaccination, including its safety and effectiveness.

The GLNPO Safety  Manager is responsible for setting up  and operating our vaccination program
which has been in effect since September  1, 1993.

Vaccinations are  performed under the supervision of a licensed physician or other health  care
professional.  Employees taking part in the vaccination program are  listed on the following pages.
Employees who have declined to take part in the program  are listed as  well, and have signed the
"Vaccination Declination Form".

To ensure that all employees are aware of our vaccination program,  it  is thoroughly discussed
during  our bloodborne pathogens  training.   We also post "Vaccination  Program  Notices" in
prominent places throughout our facility.

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                                                   SECTION: APPENDIX H
                                                   VERSION: FINAL/MAY 1997
                                                   PAGE:  19 of 32
EMPLOYEES ELIGIBLE FOR HEPATITIS B VACCINATION
NAME
                       DEPT/
                       TITLE
ACCEPTED/
DECLINED
DATES
SCHEDULED
INOCULATION
RECEIVED
  #!/   #2/   #3/

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                                                              SECTION:  APPENDIX H
                                                              VERSION:  FINAL/MAY 1997
                                                              PAGE: 20 of 32
                         VACCINATION DECLINATION FORM
DATE
EMPLOYEE NAME

EMPLOYEE ID#
I understand that due to my occupational exposure to blood or other potentially infectious materials I may be
at risk of acquiring Hepatitis B virus (HBV)  infection. I have been given the opportunity to be vaccinated
with Hepatitis B vaccine at no charge.  However, I decline to receive the  Hepatitis B vaccination at this
time.  I understand that by declining this vaccine, I continue to be at risk of acquiring Hepatitis B, a serious
disease.  If, in future, I continue to have occupational exposure to blood or other potentially infectious
materials and I wish to  be vaccinated with Hepatitis  B vaccine at that time, I can receive the vaccination
series at no charge.
EMPLOYEE SIGNATURE                                          DATE
FACILITY REPRESENTATIVE SIGNATURE                            DATE

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                          SECTION: APPENDIX H
                          VERSION: FINAL/MAY 1997
                          PAGE: 21 of 32
     MAKE SURE YOU HAVE RECEIVED YOUR

          HEPATITIS B
         VACCINATION
        The vaccination is given at the following times:
        Location:
TO SIGN UP CALL 	OR
TALK TO YOUR SUPERVISOR.

        THIS VACCINATION IS FREE OF
        CHARGE TO ALL EMPLOYEES

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                                                          SECTION:  APPENDIX H
                                                          VERSION:  FINAL/MAY 1997
                                                          PAGE  22 of 32
B. POST-EXPOSURE EVALUATION AND FOLLOW-UP

If one  of our employees is involved in an incident where exposure 1o bloodborne pathogens may
have occurred, there are two things that we immediately focus our attention and efforts upon:

• Investigating the circumstances surrounding the exposure incident.

• Making  sure  that our employees receive medical consultation and treatment if needed  and as
expeditiously as possible.
The Designated Exposure Control Officer investigates eveiy exposure incident that occurs in our
facility.  This investigation is initiated within 24 hours after  the  incident occurs  and involves
gathering the following information:

• When the incident occurred
        - Date and time

• Where the incident occurred
        - Location within the facility

• What potentially infectious materials were involved in the incident
        - Type of material (blood, etc.)

• Source of the material

• Under what circumstances the incident occurred
        - Type of work being performed

• How the incident was caused
        - Accident
        - Unusual  circumstances (equipment malfunction, power failure, etc.)

• Personal protective equipment being used at the time of the incident

• Actions taken as a result of the incident
        - Employee decontamination
        - Clean up
        - Notifications made

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                                                           SECTION:  APPENDIX H
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE: 23 of 32
After this information  is collected it is evaluated, a written summary of the incident and its cause
is prepared, and recommendations are made  for avoiding similar incidents in the future (to assist
with this gathering of information, we use the "incident Investigation Form").

In  order to ensure  that our  employees receive the best  and most timely treatment  should an
exposure to bloodborne  pathogens occur, our  facility has set up a comprehensive post-exposure
evaluation and follow-up process.  We use the checklist at the end of this section to verify  that all
the  steps in the  process have  been correctly  followed.  This  process is overseen  by the following
people:

                   •   Designated Exposure Control Officer

                   •   Ship Operations Project Officer

                   •   GLNPO Health & Safety Manager
We recognize that much of the information  involved in this process must remain  confidential, and
will do everything possible to protect the privacy of those involved.

As  the first  step  in this  process we  provide  an  exposed employee  with  this confidential
information:

• Documentation regarding  the  routes of exposure and  circumstances under which the exposure
  incident occurred.

• Identification of the source  individual (unless infeasible or prohibited by law).

Next, if possible, we test the source individual's blood to determine the HBV and HIV infectivity.
This information will also be made available to the exposed employee,  if it is obtained.  At that
timed, the employee will be  made aware of any applicable  laws and  regulations  concerning
disclosure of the identity and infected status of the source individual.

Finally, we collect and test the blood of the exposed employee for HBV and HIV  status.

Once these procedures have been completed, an appointment is arranged  for the exposed employee
with a qualified health  care professional to discuss the employee's medical status.   This  includes
an evaluation of any reported illness as well as any recommended treatment.

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                                                         SECTION:  APPENDIX H
                                                         VERSION:  FINAL/MAY 1997
                                                         PAGE: 24 of 32
C. INFORMATION PROVIDED TO THE HEALTH CARE PROFESSIONAL

To assist the health care professional we forward a number of documents to him/her, including the
following:

• A copy of the Bloodborne Pathogens Standard

• A description of the exposure incident

• The exposed employee's relevant medical records

• Other pertinent information


D. HEALTH CARE PROFESSIONAL WRITTEN OPINION

After the consultation, the  health care  professional provides our facility with a written  opinion
evaluating the exposed employee's situation.  We, in turn, furnish a copy of this medical  opinion
to the employee in question.

In keeping  with  the  emphasis  on  confidentiality,  the  written opinion  will contain only  the
following information:

• Whether a Hepatitis B Vaccination  is indicated for the employee.

• Whether the employee has received the Hepatitis B Vaccination.

• Confirmation that the employee has been told  about any  medical condition resulting from  the
  exposure incident which may require further evaluation or treatment.

All other findings or  diagnoses will  remain  confidential  and will not be included  in  the written
report.


 E. MEDICAL RECORDKEEPING

To  make sure that we have as much  medical information available to the participating health care
 professional as possible, our facility maintains comprehensive medical records on our employees.

 GLNPO Health  and Safety Manager is responsible for setting  up and maintaining these records
 which include the following information:

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                                                          SECTION:  APPENDIX H
                                                          VERSION:  FINAL/MAY 1997
                                                          PAGE: 25 of 32
        • Name of the employee

        • Social security number of the employee

        • A copy of the employee's Hepatitis B Vaccination status
                  - Dates of any vaccinations
                  - Medical records relative to the employee's ability to receive the
                    vaccination

        • Copies of  the results  of the examinations,  medical  testing, and  follow-up  procedures
         which look place as a result of an employee's exposure to bloodborne pathogens.

        • A  copy of the information provided to the consulting health care professional as a result
         of any exposure  to bloodborne pathogens.

As  with all information  concerning  these areas,  we  recognize that  it is important to  keep  the
information  in these  medical records confidential.  We will not disclose or report this information
to anyone without the employee's written consent except as required by law.

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                                                         SECTION:  APPENDIX H
                                                         VERSION:  FINAL/MAY 1997
                                                         PAGE: 26 of 32
EXPOSURE INCIDENT INVESTIGATION FORM

OATH OF INCIDENT 	TIME OF INCIDENT-

LOCATION
POTENTIALLY INFECTIOUS MATERIALS INVOLVED.

TYPE:	SOURCE




CIRCUMSTANCES (WORK BEING PERFORMED, ETC )• 	
FIOW INCIDENT WAS CAUSED (Accident, equipment malfunction, etc )
PERSONAL PROTECTIVE EQUIPMENT BEING USED
ACTIONS TAKEN (Decontamination, clean up, reporting, etc )
RECOMMENDATIONS FOR AVOIDING REPETITION OF INCIDENT

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                                                        SECTION:  APPENDIX H
                                                        VERSION:  FINAL/MAY 1997
                                                        PAGE:  27 of 32
POST-EXPOSURE EVALUATION AND FOLLOW-UP CHECKLIST

The following steps must be taken, and information transmitted, in case of an employee's exposure
to bloodbome pathogens:
       ACTIVITY                                      COMPLETION DATE

Employee furnished with documentation                     	
regarding exposure incident.
Source individual identified.
       Source Individual

Source individual's blood tested and results
given to exposed employee.
       _ Unable to obtain consent.

Exposed employee's blood collected and

Appointment arranged for employee with
  health care professional.
       Professional's Name
Documentation forwarded to health care
professional.
                        _Bloodborne Pathogens Standard
                        _Description of exposed employee's duties
                        _Description of exposure incident, including    routes of exposure
                        _Result of source individual's blood testing
                        _Employee's medical records

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                                                          SECTION: APPENDIX H
                                                          VERSION: FINAL/MAY 1997
                                                          PAGE:  28 of 32
SECTION VI

LABELS AND SIGNS

For  our employees  one  of the most  obvious warnings  of possible  exposure  to bloodborne
pathogens are biohazard labels.  Because of this, we have implemented a comprehensive biohazard
warning labeling program in our facility using labels of the type shown on the following page or,
when appropriate,  using red "color-coded"  containers.   The  Designated  Control  Officer  is
responsible for setting up and maintaining this program in our facility

The following items in our facility are labeled:

• Containers of regulated waste

• Refrigerators/freezers containing blood or other potentially infectious
  materials. (MEDICAL)

• Sharps disposal containers.  (MEDICAL)

• Other containers used to store, transport or ship blood and other infectious materials.

• Laundry  bags and containers

• Contaminated equipment

On labels  affixed to  contaminated  equipment we have  also indicated which  portions  of the
equipment  are contaminated.

We  recognize that biohazard signs must be posted  at  entrances to HIV  and HBV research
laboratories and production facilities.  However, we do not have these types of operations in our
facility  so we are not affected by these special requirements

(If the  facility has HIV and HBV research laboratory and production operations,  please see the
copy of the Bloodborne Pathogens Standard, section (g) (1) (ii) for warning sign requirements.)

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                                                         SECTION: APPENDIX H
                                                         VERSION: FINAL/MAY 1997
                                                         PAGE: 29 of 32
BIOHAZARD LABELS

SECTION VII

INFORMATION AND TRAINING INFORMATION AND TRAINING

Having  well  informed  and educated  employees is  extremely important when  attempting to
eliminate or minimize  their exposure to bloodborne pathogens.  Because of this,  all  employees
who have the potential  for exposure to bloodborne pathogens must participate  in a comprehensive
training program and are furnished with as much information as possible regarding this issue

This program was set up so that employees would receive the required training on or before June
4,  1992.  Employees will  be  retrained at least annually to keep their  knowledge up to  date.
Additionally, all new employees, as well  as those changing jobs or job functions,  will be given
any additional training that their new position may require at that time.
The GLNPO Health and Safety Officer is responsible for seeing that all employees who have the
potential for exposure to  bloodborne  pathogens receive this training.  He/she will be assisted by
the following instructors'
A. TRAINING TOPICS

The topics covered in our training program include, but are not limited to. the following'

       • The Bloodborne Pathogens Standard.

       • The epidemiology and symptoms of bloodborne diseases.

       • Our facility's Exposure Control Plan  (and where employees can obtain a
         copy).

       • Appropriate methods for recognizing  tasks and other activities that may involve exposure
         to blood and other potentially infectious materials

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                                                           VERSION:  FINAL/MAY 1997
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       • A review of the use and limitations of methods that will prevent or reduce exposure,
         including:

             - Engineering controls
             - Work practice controls
             - Personal protective equipment

       • Selection and use of personal protective equipment including:
             - Types available
             - Proper use
             - Location within facility
             - Removal
             - Handling
             - Decontamination
             - Disposal

       • Visual  warnings  of  biohazards  within  our  facility  including  labels,  signs,  and
         "color-coded" containers.

       • Information on the Hepatitis B vaccine, including:
         - Efficacy
         - Safety
         - Method f administration
         - Benefits of vaccination
         - Our facility's free vaccination program

       • Actions  to take  and persons to  contact in  an emergency  involving blood  or other
         potentially infectious materials.

       • The procedures to follow if an exposure incident occurs, including incident reporting.

       • Information  on  the  post-exposure  evaluation  and  follow-up,  including   medical
         consultation,  that our facility will provide.

B. TRAINING METHODS

Our  facility's  training  presentations make use of several  training  techniques  including,  but not
limited to:

       • Classroom type atmosphere with personal instruction.
       • Video programs.
       • Training manuals/employee handouts
       • Employee review sessions.

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                                                        SECTION:  APPENDIX H
                                                        VERSION:  FINAL/MAY 1997
                                                        PAGE:  31 of 32
We have used the forms found on the following page and/or our computer systems to facilitate this
rccordkeeping.

These training records  are available for examination and  copying  to  our  employees and their
representatives  as well as to OSHA and its' representatives.
                  BLOODBORNE PATHOGENS TRAINING SESSIONS
DATE OF SESSION
                                 _SESSION SUMMARY (A'1TACHE-J)_
INSTRUCTOR(S)
QUALIFICATIONS.
ATTENDEE NAME    JOB TiTI V.      SSN
                                                       SIGNATURE

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                                                         SECTION:  APPENDIX H
                                                         VERSION:  FINAL/MAY 1997
                                                         PAGE:  32 of 32
SECTION VIII

HIV AND HBT RESEARCH LABORATORIES AND PRODUCTION FACILITIES

We  recognize  that there  are several  special  requirements  for  HIV  and  HBV  research  and
production facilities in the areas of construction, engineering controls, work practices, the use of
containment equipment as  well as employee education and training.  However,  since we do not
have these types of operations in our facility, these special requirements  do not apply.  Therefore,
our exposure control plan does not address these requirements.

(If your facility includes HIV  or HBV research laboratories or production facilities, you can find a
listing  of special requirements that the Standard  imposes upon such facilities by consulting the
Bloodbome Pathogen  Standard  29 CFR  1910.1030.   In this  case, to  complete your Exposure
Control Plan you will need to document the methods you will use to comply with these special
requirements as well as your implementation schedule.)

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                                                          SECTION: APPENDIX I
                                                          VERSION'  FINAL/MAY 1997
                                                          PAGE: Page 1 OF 9
APPENDIX I:  PERSONAL PROTECTIVE CLOTHING & EQUIPMENT

Purpose

This section establishes policy, responsibilities, and practices for the procurement, issue, control,  and
use of  protective  clothing and  equipment by GLNPO employees engaged  in  laboratory and  fiel '
activities.
Policy

It is the  policy of the  Environmental Protection Agency to administer its programs in a manner that
will assure the protection of the health and safety of all personnel in routine field and laboratory work,
especially those persons engaged  in work of a hazardous or toxic  nature,  by providing and  requiring
the use of specified protective clothing and equipment.
References

        1.      Occupational Safety and Health Act, P.L. 91-596.
        2.      29 CFR 1910, Subpart I. Sections 132, 133, 134, 135, 136, 137, and 138.
        3.      EPA Order 1440.2 Health and Safety Requirements for Employees engaged in Field
               Activities, EPA Order 1440.3 Respiratory Protection.
        4.      Executive Order 12196, Occupational Safety and Health for Federal Employees, 1980.
Definitions

The applicable terms used in this chapter are defined in the following paragraphs.


Field Activities

The term  field activities, as used in this chapter, means EPA program activities that are conducted by
EPA employees outside of EPA administered facilities.  These activities include, but are not limited to,
environmental and pesticides sampling, field analysis, inspection of water and wastewater treatment
plants, hazardous  material spills and waste site investigations, inspections, and sampling.


Routine Laboratory Activities

The  term  routine laboratory activities denotes EPA  program  activities that are  conducted by EPA
employees in an EPA-administered laboratory facility.

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                                                            SECTION: APPENDIX I
                                                            VERSION   FINAL/MAY 1997
                                                            PAGE: Pasic 2 OF 9
Applicability

The provisions of this chapter are applicable to all GLNPO employees at all operational  levels.  They
are also applicable to EPA contractors.
Responsibilities

The  duties  assigned  Regional personnel with regard to  Personal  Protective Equipment  (PPE),  are
detailed in the following paragraphs.
Regional Administrator

The Regional Administrator is responsible for implementing the regional safety program of which the
requirements of this chapter are a part.  It is the responsibility of the Regional Administrator to require
Division and  Office Directors to budget the  funds  necessary to procure personal protective clothing
and equipment.
Division and Office Directors

The Division and Office Directors  are  responsible for assigning staff to  field and laboratory work
which may require the use of personal protective clothing and safety equipment.
Supervisors

In accordance  with  the procedures of this  manual, the  supervisor is  responsible for determining the
level of protection required (in consultation with the Health and Safety Officer).  He/she is responsible
for controlling, issuing,  and inspecting the protective clothing/equipment in their section.  He/she is
responsible for identifying employees who  require protective prescription lenses and for having these
employees provide current prescriptions.  He/she is responsible for keeping an accurate inventory and
for maintaining the  protective clothing/equipment in a functional condition.  He/she  is responsible for
identifying employees who require training  and certification; for ensuring that those employees receive
training and certification in compliance with the provisions of EPA Orders 1440.2, 1440.3, and OSHA
29 CFR 1910.120 and 1910.132 through 139;  and for ensuring these requirements are contained in
their  position  description.   He/she  is responsible for  recommending appropriate  correction  and/or
disciplinary action of employees who choose to violate or neglect safety requirements.

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                                                           SECTION: APPENDIX 1
                                                           VERSION:  FINAL/MAY  1997
                                                           PAGE: Page 3 OF 9
Regional Health and Safety Manager

The  Regional  Health  and Safety Manager  (RHSM)  is  responsible for assisting supervisors  in  the
selection, procurement,  issuance, and  maintenance of protective  clothing  and equipment.   He/she
reviews every  procurement request  related to safety,  health  or  personal  security, to assure that  the
request  is justified and appropriate.  He/she will determine if the item, or  an acceptable alternative, is
available in the Region, based on the Regional  inventory of all  protective clothing/equipment that
he/she maintains.  He/she will annually perform a review and audit of the  condition, inventory, and
use  of protective  clothing/equipment  for each  Division  and  Office.   A  report  of this annual
review/audit will be distributed to all Division/Office Directors.  He/she is responsible for identifying
program areas that* require  training  and certification.   As  a  member  of the  Region 5  training
committee,  he/she  advises  the  Office  of  Personnel  regarding  the  Health  and   Safety  training
requirements of EPA  1440.2,  1440.3,  and OSHA 29 CFR  1910.120, and recommends the  required
funding.   The  Regional Health and  Safety manager  shall  be  responsible  for administering  the
Respiratory Protection  Program in Region 5.
Employee

Employees  are  responsible for  the  protective  clothing  and/or  equipment  issued  to  them  and are
required  to  wear and use the clothing and  equipment as prescribed in this chapter and for reporting
any damage and/or malfunction of the clothing/equipment issued to them. Employees  may provide a
current prescription, at their own expense,  if the supervisor has  determined  that the government will
provide protective prescription eye wear.

Employees assigned protective clothing and/or equipment are required to sign a receipt  for such items,
verifying that:
    •/  o

        1.      They have received proper training in the use and maintenance of these items.

       2.      They have read the safety procedures and agree to  accept the responsibilities provided
               therein.

       3.      They accept responsibility  for  the  maintenance  and  use of the assigned protective
               clothing and  equipment.   Cleaning, sanitizing  and  maintaining  the  respirators  is
               included in  this  responsibility.  Any damage,  excessive  wear, or malfunction  of the
               equipment must  be reported immediately to the  individual's supervisor.  Individuals
               are also responsible for the cost of replacing items of protective clothing or equipment
               lost, damaged, or stolen through their own negligence.

       4.      Items provided from  a general  supply  will be maintained by the  organizational unit
               responsible for its supply and issuance.

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                                                           SECTION: APPENDIX I
                                                           VERSION:  FINAL/MAY  1997
                                                           PAGE: Pa«e 4 OF 9
Facilities Engaged in Hazardous Field Work

Each facility regularly engaged  in hazardous, or  potentially hazardous, field work will be responsible
for:

        1.      Maintaining adequate  supplies of disposable  coveralls,  gloves,  respirators  and their
               replacement cartridges, and overboots.

        2.      Providing lockable cabinets for storage of PPE in the field.

Personal Protective Equipment Requirements

It  is the policy of  GLNPO to  determine the level of protection  required  for employees  assigned
specific duties, to  provide adequate and appropriate PPE, and  to enforce its proper  use.  Additionally,
specific  information on  related  PPE considerations may be obtained through the use of the EPA's
"Guidelines for the Selection of Chemical Protective Clothing" and through  consultation with Regional
Safety  and Health  Manager.

GLNPO requires  the  use  of PPE whenever there  are  hazard  exposures  that  can  be eliminated or
protected against through the use of PPE.   No  unprotected person shall  knowingly  be  subjected to
hazardous environmental conditions. All laboratory and field operations should be evaluated, and their
respective  hazards assessed, to  assure that adequate  and appropriate  PPE  is selected, and  its  use  is
enforced.

Eye and Face Protection

Descriptions of the protective equipment available specifically  for the eyes and face  are included in the
following paragraphs.

Protective Eye Wear

Protective  eye wear,  including  plain  and  prescription lenses,  shall be  provided to all  field  and
laboratory  employees. They must be worn when the  danger of eye injury exists. No one may enter a
controlled  location where eye protection is mandatory without such equipment.  Areas in  a laboratory,
or on-site  locations may be designated as  controlled areas by the person in  charge.   It  is his/her
responsibility to determine the level  of protection required and to enforce its use.
Safety Glasses

Protective  eye and face devices purchased after July 5,  1994 shall  comply with  American  National
Standards  Institute  (ANSI)   Standard  Z87.1-1989,  "American  National  Standard  Practice  for
Occupational  and Educational Eye  and Face Protection"; eye and face protective devices purchased
before  July   5,  1994  shall  comply  with  the ANSI  Standard  Z87.1-1968,  "USA  Standard  for
Occupational  Eye and Face Protection".

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                                                            Sf-CTION: APPENDIX I
                                                            VERSION-  FINAL/MAY 1997
                                                            PAGE: Pace 5 OF 9
Safety Goggles

A variety of goggles are available to protect the eyes from flying  particles, liquids,  gases and vapors,
however,  those types  of goggles are  hazards-specific  making proper  selections critical to employee
protection.

Use of the appropriate type of goggle for eye protection is required when safety glasses do not provide
adequate protection against the hazards encountered in the work area.

Face Shields

Safety  glasses or goggles offer little protectio,. (.0 the face and neck.  Full face shields that protect the
face  and neck  shall be worn  when  the maximum protection  from flying particles and  splashes from
harmful liquids is needed.   Face shields are  not  a primary eye protection device and are designed for
use over the  protective eyewear  required for the operation.

Contact Lenses

Contact lenses are not protective eyewear.  In  situations where protective eyewear is  required,  the
manager or  supervisor in  consultation with the GLNPO Safety Manager will determine  if contact
lenses may be worn with  additional appropriate eye protection as determined by 29 CFR  1910.134.
Contact lenses may not be  worn where there  is risk of exposure to:  intense heat;  molten  metals; high
particulate containing atmospheres; chemical  fumes, vapors or splashes;  or while wearing a respirator.

Head Protection

All  head  protection purchased  after July 5, 1994, shall  comply  with ANSI Standard  Z89.1  1986,
"American National Standard  for Personnel Protection — Protective Headwear for Industrial  Worker —
Requirements"; protective headwear  purchased before July 5, 1994 shall comply with  the Requirements
for Industrial Head Protection.  Head protection will be worn  by all GLNPO employees where there is
danger of injury from  limited  electric shock or from falling or flying  objects.  Head  protection is
required  at waste-clean up sites where injury may result from equipment  operation. Employees  are
required  to  wear head protection  during all  inspections  of industrial  and  chemical  plants,  waste
disposal operations, and sites where any of the above hazards exist.

Protective Apparel

Protective  apparel is required for most field  and laboratory work performed in the Region.  It should
be strong,  resistant to  chemical  and  thermal  penetration, flexible  and easy to clean.   The performance
requirements are to be determined  by  the substances being  handled.  It is the responsibility of the
supervisor to determine the choice of garment to be worn (laboratory coat, coveralls, rubber or plastic
aprons,  splash suits, fully  encapsulating disposable  coveralls, Personal  Floatation   Devices (PFD's),
etc.)

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                                                           SECTION: APPENDIX I
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE: Page 6 OF 9
Aprons, Coveralls, Splash Suits

Plastic  or  rubber aprons,  coveralls or  splash suits  provide protection  from corrosive or  irritating
liquids.  However, they can accumulate  a charge of static electricity  and in the case of fire can cause
additional injuries.  Therefore, they should not be worn in  areas where flammable  solvents or other
materials could be ignited by a static charge.
Flame Resistant Garments

Properly-designed pants,  jackets  and hard hat  liners  with appropriate  chemical  treatment  provide
protection  from  fire  hazards such as unexpected" uimace  flames and from the hazard  of splashed
molten  metal.   Jackets with  pockets on the  inside only  help  to prevent injury in the  presence  of
high-temperature, airborne particles.  Similarly, properly fitted jackets and pants, which are  not rolled
up, can also prevent injury.   Employees  are  required to wear  chemically  treated  jackets  and  pants
during  all  coke  oven  battery   and  blast  furnace  casthouse  inspections.     At  a  minimum,
chemically-treated jackets must be worn during inspections at basic oxygen furnace  shops, electric arc
furnace shops and facilities with  electric arc  furnaces and  cupolas.  It is important that  this type  of
protective clothing be chemically-treated after  each washing,
Disposable Outer Garments

Disposable  outer garments (e.g.  Polyethylene, Saran  Coated or  Tyvek)   may,  in  some  cases, be
preferable  to reusable  ones.   Examples  include  the handling  of quantities of highly  toxic or
carcinogenic materials.   Disposable  full length suits are recommended  for  high risk situations;
however,  many disposable garments  offer limited  protection  from  vapor penetration  and their  use
should be determined by the supervisor.
Disposal of Contaminated Garments

Garments should be disposed of in accordance with disposal and decontamination procedures provided
by the provisions of the Specific Site Safety Plan or SOP or similar rule.
 Gloves

 Gloves will be worn when it is necessary to handle corrosive materials,  sharp or rough objects, hot or
 cold materials, or when there is a possibility of exposure to chemicals.  Gloves will not be worn near
 machinery if they may cause bodily injury as a result of being caught in  the machine.

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                                                           SECTION: APPENDIX I
                                                           VERSION.  FINAL/MAY 1997
                                                           PAGE: Pace 7 OF 9
The following additional requirements will be met:

        1.      Gloves  will be  selected based on their intended use,  the  hazard  involved and their
               suitability for the job.

        2.      Before use, gloves will be inspected for punctures, tears or discoloration.

        3.      Gloves will be appropriately cleaned before removal.

        4.      Leather  gloves  will  be used  for handling broken  glassware,  for  inserting  rubber
               stoppers into glass tubes, for  protection against rough  or sharp-edged  objects,  hot  or
               cold materials  and  for  similar operations  where protection from chemicals  is not
               needed.

        5.      Specialized gloves are manufactured for specialized activities.  These activities include:

               A.      Electrical  operations  or  fish  shocking  activities where the  use  of  rubber
                       insulated gloves meet OSHA regulations.

               B.      Working at  temperature  extremes  where  the use of gloves  made with such
                       materials as  Nomex or  Kevlar or  in  combination  with  other materials  is
                       required.

Foot Protection

All safety footwear  purchased  after July  5,  1994,  shall  comply with ANSI  Standard  Z41-1991,
American National Standard  for Personnel  Protection - Protective Footwear";   protective footwear
purchased before July 5, 1994,  shall  comply with the  ANSI  Standard Z41.1-1967,  "USA Standard for
Men's Safety-Toe Footwear".  Shoes must be worn at all times in areas where chemicals are stored  or
used.    Perforated  shoes or  sandals must  not  be worn in laboratories or  during  any  field  work.
Safety-toe  shoes  must be worn  by  all GLNPO employees who regularly engage  in moving, lifting,
handling or  carrying supplies,  materials, equipment,  furniture,  or other objects of such weight that
accidental injury  to the toes from falling or shifting of such objects may result.  Foot protection, other
than ordinary  shoes, may be  required  in  special cases.   Safety-toe  shoes or  safety-toe boots are
required for  most field activities. Rubber boots or plastic shoe covers  are to be  worn  over safety-toe
shoes to avoid possible exposure to corrosive chemicals or to large quantities of solvents or water that
may penetrate normal foot gear  (e.g., during clean-up operations.)

Respiratory Protection

Approved  respirator protection  will  be  \\orn by GLNPO  employees  when  they are working  in,  or
when they encounter, hazardous atmospheres exceeding the Permissible Exposure Limits, in suspected
oxygen  deficient atmospheres,   or  where  there  is  imminent  danger of  release  of  airborne  toxic
substances.  See Appendix A,  Respiratory Protection Program, of this manual.

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                                                          SECTION- APPENDIX I
                                                          VERSION: FINAL/MAY 1997
                                                          PAGE: Page 8 OF 9
Non-Personal Protective/Safety Equipment in Laboratories

Emergency alarm  systems, safety showers,  eye wash  fountains,  fire extinguishers,  and other such
equipment will be provided as part  of the laboratory  work place.   The requirements  and technical
standards are set forth in 29 CFR 1910.1450,  EPA  1440 "Occupational Health and Safety in EPA
Regions: Program  Guidance", EPA Safety Manual, and GSA Safety and Environmental  Management
Program (PBS- 1L-92-4).

All laboratory safety and emergency equipment should be inspected no less than once every 6 months;
the SCBAs should be inspected after every use or not less than once every month.

Emergency Equipment

The following equipment is required for  laboratory emergencies.  This equipment should  be  stored
together in  a central  location  closest  to the  laboratory  operation  having the highest risk  of an
emergency situation.

        Quantity                                    Description

              2                           Positive  pressure   (pressure  demand)  self-contained
                                           breathing   apparatus   (SCBA)   (MSHA,   NIOSH
                                           approved)

              2                           Fully encapsulated suits*

              2                           Hooded, chemical splash suits

              2                           Chemical resistant disposable coveralls

               1                           Spill control cart with spill control equipment

               1                           Thin-window Geiger counter (0-5mr/hr.) minimum*

                                           *Optional

Several pairs of  gloves are also required. The gloves should be selected to  protect against a variety of
corrosive or toxic materials.  Leather  and insulated gloves should also be included in the selection.

All of the above equipment should be inspected periodically  (at  least  every  six months.)  The SCBAs
should be inspected after each use or  at least once a month.

Additional Protective Requirements

To prevent  injury  and protect the skin,  laboratory  personnel may  not wear loose, ragged or torn
clothing and  laboratory coats, or meager or insufficient clothing (e.g., shorts and/or halter tops).

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                                                            SECTION: APPENDIX I
                                                            VERSION:  FINAL/MAY 1997
                                                            PAGE: Pa»e 9 OF 9
Issuance and Training  in the Use of Protective Clothing and Safety Equipment

All of the protective clothing and  equipment listed in this manual, as specified for  use in  laboratory
work or field operations must be issued to the employee or made available.  If PPE is required  for the
task,  it must be transported to the point of operation  and be worn and/or utilized as necessary in
performing  the activity.  For laboratory work,  clothing and equipment made available on site satisfies
the intent of this manual.
Standard Level of Protection Provided

The Agency will provide each employee regr'^rly engaged  in field work with the following:  safety
toe shoes;   or toe caps, safety  glasses/goggles and face shield  (prescription  safety glasses  will be
provided when safety goggles do not fit over regular prescription glasses), and an approved hard hat.
Field Work of a Hazardous Nature

In addition to the above, the Agency will provide each employee assigned to field work of a hazardous
or potentially hazardous nature  the following items:   disposable coveralls, disposable gloves, pull on
safety toe rubber boots (knee high), and a respirator (as appropriate.)
Specialized Field Work

The  Agency will  provide personnel assigned to specialized field work  (e.g., GLNPO and  CERCLA
personnel)  work  clothing/equipment as necessary.  This may include:  life preserver;  exposure  suit;
waders, or fully  encapsulated protective suit.  Employees assigned to field work,  when there is the
potential of fire,  will be  issued fire resistant coveralls and jackets (e.g., Nomex.)  Those  employees
who require respiratory  protection and prescription lenses will be provided prescription lenses mounted
in a special frame to accommodate the respiratory equipment.
Training

All employees engaged  in field  work will receive training  and certification in compliance  with  the
provisions of EPA  Order 1440.2 and 1440.3.  Employees shall not be permitted to engage in routine
field activities unless they have been trained and certified to a level commensurate with the degree of
anticipated hazards.  All employees shall be provided  with  a minimum  of 24 hours  of health  and
safety  training prior to their  becoming involved in normal routine field  activities.   In  addition,
employees engaged in  field  activity requiring  the  use of  respiratory  protective devices  must  be
properly trained in  the selection and use of such devices and certified  that  he/she is physically capable
of wearing such equipment.

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                                                       SECTION: APPENDIX J
                                                       VERSION: FINAL/MAY 1997
                                                       PAGE 1 of 29
APPENDIX J;        CHEMICAL HYGIENE PLAN AND SAFETY MANUAL

TABLE OF CONTENTS

1.1            Introduction

1.2            General Principles

1.3            Responsibilities

1.4            The Laboratory Facility

1.5            Basic Rules and Procedures for Working with Chemicals

1.6            Chemical Hygiene Standard Operating Procedures

1.7            Chemical Procurement, Distribution, and Handling

1.8            Signs and Labels

1.9            Hazard Identification

1.10          Industrial Hygiene

1.11          Employee Information and Training

1.12          Medical Evaluation Program

1.13          Personal Protective Equipment

1.14          Recordkeeping

1.15          Housekeeping, Maintenance, and Inspection

1.16          Waste Disposal

1.17          Spills and Accidents

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ATTACHMENTS

Attachment 1   -

Attachment 2   -

Attachment 3   -

Attachment 4   -

Attachment 5   -

Attachment 6   -

Attachment 7   -
                                                      SECTION:  APPENDIX J
                                                      VERSION:  FINAL/MAY 1997
                                                      PAGE 2 of 29
29 CFR 1910.1450, Revised July 1, 1992

Storage For Chemical Compatibility

SOP for Hazardous Material Storage and Spill Prevention

Weekly Inspection Checklist

SOP for Hazardous and Common Wastes

Radiation Safety For R/V Lake Guardian Laboratories

CRL Radiation Safety Manual

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                                                         SECTION-  APPENDIX J
                                                         VERSION:  FINAL/MAY 1997
                                                         PAGE 3 of 29
     R/V LAKE GUARDIAN CHEMICAL HYGIENE PLAN AND SAFETY MANUAL
                  U.S. EPA, Region 5, Great Lakes National Program Office
                            Surveillance & Research Staff (SRS)
                                  77 West Jackson Blvd.
                                  Chicago, Illinois 60604
1.1    INTRODUCTION

The Environmental Protection Agency (EPA) has approximately 33 laboratories in different areas
of the country.   At these laboratories  EPA employees analyze samples and conduct  tests  in
support of the investigative, enforcement, and research responsibilities  of Superfund and other
Agency Programs.  Analysts are  typically involved with samples  or tests containing hazardous
substances or constituents.

The  Occupational   Safety  and Health  Administration's (OSHA)  regulation for "Occupational
Exposures to Hazardous Chemicals in Laboratories",  29 CFR 1910.1450 (the standard) requires
that each facility engaged in the laboratory use of hazardous chemicals develop and implement a
written program known as  a "Chemical  Hygiene Plan" which sets forth procedures,  equipment,
personal protective equipment and  work practices that are capable of:

       •      protecting employees  from the health  hazards presented  by hazardous chemicals
              used in  that  particular workplace; and

       •      keeping employee  exposures to the hazardous chemicals,  to  which they  may  be
              occupationally exposed in their laboratories, below the permissible  exposure limits
              specified in  the  standard.
Purpose

This  Chemical  Hygiene  Plan  (CHP)  sets  forth policies,  procedures,  equipment,  personal
protective equipment,  and work  practices that  when  properly  implemented,  are capable of
protecting employees from the health hazards presented by hazardous chemicals used in the Lake
Guardian  laboratories.   It  is the  basis  for a chemical hygiene program  to  ensure the proper
implementation of controls to protect the safety and health of personnel working in Great Lakes
National Program Office (GLNPO) laboratories.

This Plan is intended to meet the requirements of 29  CFR 1910.1450, the OSHA's standard for
occupational exposures  to hazardous  chemicals in laboratories, a  copy  of which is  found in
Attachment 1.

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                                                         SECTION:  APPENDIX J
                                                         VERSION:  FINAL/MAY 1997
                                                         PAGE 4 of 29
Scope

All individuals  employed  in this  laboratory workplace  who  may be  exposed  to  hazardous
chemicals in the  course of their assignments are required to follow the provisions of this Chemical
Hygiene Plan.

The Chemical Hygiene  Plan will be reviewed  and updated annually by  the  Chemical Hygiene
Officer and the Region V Safety Officer.
1.2    GENERAL PRINCIPLES

The  following  General  Principles will  be adhered to in the  implementation  of this Chemical
Hygiene Plan.

       1.       Minimize all chemical exposures
               - Laboratory activities can expose employees to a number of different chemicals at
               relatively small  exposure levels.  It is our intent  to minimize these exposures to
               the lowest possible levels.

       2.       Minimize risk
               - Avoid underestimation of risk
               -  General  safety procedures  are  developed  to  account for  health  risks  in
               performing various procedures.

       3.       Provide adequate ventilation
               -  General and local ventilation (through use of fume hoods) will be  used as
               recommended  in 29  CFR  1910.1450  a  copy  of  which  can be  found  in
               Attachment 1.

       4.       Institute a Chemical Hygiene Program
               -  A Chemical Hygiene  Program has been incorporated  with  existing Laboratory
               Standard  Operating Procedures and Safety Manuals.

       5.       Observe established Action Levels and/or Permissible Exposure Limits (PELs) and
               Threshold Limit  Values  (TLVs).  Monitoring will be done as necessary to assure
               safe levels of chemical

       6.      Properly  dispose of hazardous chemicals
               -  Chemical  waste will  be  properly  disposed of according to applicable federal,
               state, and local regulations.

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                                                          SECTION: APPENDIX J
                                                          VERSION: FINAL/MAY 1997
                                                          PAGE 5 of 29
1.3    RESPONSIBILITIES

General laboratory  responsibilities are addressed  in the GLNPO Quality Assurance Project  Plan
(QAPP).    This  section  will  outline responsibilities  of  the  Chemical  Hygiene Officer  and
implementation of the Chemical Hygiene  Plan.  Documents mentioned below can be found in the
safety office.

1.     Organizational Structure
       - See  Quality Assurance Project  Plan (QAPP), Section  2.0,  Project Organization and
       Responsibility, available in the safety office.

2.     Roles and Responsibilities

       a.  EPA - See EPA 1440.5A, Section 7.a.

       b.  Project Director - See QAPP, Section 2.0.

       c.  Safety and Health Manager - See EPA  1440.5A, section 8a.

       d.  Chemical Hygiene Officer (CHO)

               •      Provide  technical  assistance/guidance   to   administrators,   laboratory
                     directors and   supervisors in  developing and implementing  chemical
                     hygiene procedures and practices.

               •      Monitor procurement, use and disposal of chemicals in the laboratories.

               •      Maintain an up-to-date file of material safety data sheets or appropriate
                     alternative.    Maintain  an  inventory of  all   hazardous  chemicals  in an
                     up-to-date manner.

               •      Assist laboratory managers or project directors to develop and implement
                     the use  of adequate procedures and use of protective equipment to ensure
                     employee health.

               •      Know the current legal  requirements with respect to the Chemical Hygiene
                     Plan (CHP), regulated substances, and disposal methods.

               •      Coordinate  emergency procedures and fire department activities  related to
                     hazardous chemicals.

               •      Conduct annual audits  to determine the  implementation and effectiveness
                     of the CHP and compliance with company policy.

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                                                   SECTION:  APPENDIX J
                                                   VERSION:  FINAL/MAY 1997
                                                   PAGE 6 of 29
       •      Ensure up-to-date records are maintained on  training of all  employees
              required to handle hazardous chemicals.

       •      Periodically   inspect  engineering  controls   and  personal  protective
              equipment.

       •      Make  routine surveys of the work area to ensure safe practices are being
              followed.

       •      Ensure  manufacturers '/suppliers'   labels  have  not  been  defaced  or
              removed.

       •      Ensure that a copy of the Chemical Hygiene:  Plan and the Material Safety
              Data Sheet  (MSDS) manual  are  readily accessible to the  laboratory
              employee.

       •      Enforce applicable safety and health rules.
e-  Employees - Lab worker/staff.

       •      Obey established safety rules.

       •      Follow established Standard Operating  Procedures when performing any
              work with hazardous chemicals.

       •      Know where the CHP and the MSDS's are kept in the laboratory.

       •      Use engineering controls and personal protective equipment as required by
              laboratory standard operating procedure and Chemical  Hygiene Standard
              Operating Procedures.

       •  Inform your supervisor of:
              Any symptoms of overexposure  that may possibly  be related  to hazardous
              chemicals;
              Missing labels on containers;
              Malfunctioning safety equipment.

       •  Do not remove or deface labels on the containers.

       •      Know the location of, and how to use, emergency  equipment,  first  aid
              supplies, emergency eyewash, engineering control devices, etc.

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                                                          SECTION:  APPENDIX J
                                                          VERSION:  FINAL/MAY 1997
                                                          PAGE 7 of 29
               •      Know your role in emergency procedures, including your assignment on
                      the ship's station bill.

               •      Know the emergency evacuation route from the laboratory.

       f. Master. Research Vessel. Lake Guardian

               •      Ensure  that all  ship's operations  are  conducted in accordance with  all
                      applicable safety and health rules and procedures.

       g.  EPA Supervisors

               •      Be sure that all laboratory employees have been properly trained regarding
                      use of  hazardous  chemicals, engineering control equipment,  personal
                      protective equipment, and waste and storage management.

               •      Ensure that employees and contractors are following the CHP.

               •      Provide assistance to the CHO in maintaining the chemical inventory  and
                      MSDS manual.

               •      Plan laboratory procedures  in a manner which will minimize employee
                      hazard and waste generation.


1.4    THE LABORATORY  FACILITY

Design

1.     Design of the laboratory facilities shall provide, at a minimum, the following components:

       a       General ventilation shall be installed to  supply 4 to  12 air exchanges per  hour.
               Positive pressure shall be maintained when necessary to reduce analytical  error
               caused  by contamination from engine exhaust fumes.  Air vents shall be altered as
               necessary to minimize  turbulence at the face of fume hoods.

       b.      Each laboratory shall be equipped with at least one chemical fume hood having a
               face velocity of 100 fpm with a minimum sash opening of 17 inches.

       c.      Each laboratory shall be equipped with an eyewash fountain capable of providing
               15 minutes of flow.  Emergency drench showers  shall be installed in numbers
               sufficient to be accessible within  10 feet of at least one exit from each laboratory.

       d.      Space for waste disposal containers  should be designed into every lab along with
               drains and openings in work surfaces to allow easy access to containers.

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2.
Designated areas.
       Maintenance.
       Usage.
                      Fume hoods #4 in the Biology Lab, #6  in the Primary Productivity  Lab,
                      and #9 and #10 in the Extraction Lab are the only designated areas for use
                      of carcinogens and highly toxic materials.  These materials are listed on
                      page J-13 of  the Chemical Hygiene  Plan.   These  materials can only be
                      used in these designated areas.
                      Chemical-hygiene-related  equipment shall  be inspected  according to the
                      schedule given in Section 1.15.   The EPA  Region V Health and Safety
                      Officer shall  be  notified  of any deficiencies  that  cannot  be easily
                      remedied.
                      Only those procedures designated by EPA GLNPO as appropriate to the
                      facilities shall be carried out in the laboratories.
       Ventilation
        Electrical
                      General  ventilation shall  not  be  used to  for  protection  from toxic
                      substances.   If fume hoods or other local ventilation  is inadequate, EPA
                      GLNPO shall be notified so that appropriate equipment is obtained.  Any
                      alteration of the ventilation system should be made only if thorough testing
                      indicates  that  worker  protection  from  airborne  toxic  substances  will
                      continue to be adequate.
                      In the Biology, Multi-Purpose (Wet), and Chemistry Labs, the outlets near
                      any   water  source  shall  be  equipped with a  Ground  Fault  Circuit
                      Interrupter (GFCI).   The  GFCI outlets shall be  inspected by  the  ship's
                      electrician before and after
                      each  survey.

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1.5    BASIC RULES AND PROCEDURES FOR WORKING WITH CHEMICALS

The following shall be used for guidelines in the general use of chemicals:

1.     Avoidance of Routine Exposure

               •       Develop  and  encourage  safe  habits;  avoid unnecessary  exposure  to
                      chemicals by any route of entry.

               •  Do not smell or taste chemicals.

               •       Apparatus which may produce toxic chemicals should be vented to prevent
                      entry into the local ventilation system.

               •       Inspect gloves and other personal protective equipment before use.

               •       Do not allow release of toxic substances in cold rooms (walk-in coolers)
                      which may be inadequately ventilated.

2.     Use of Chemicals

               •       Use only those chemicals for which the ventilation system is appropriate.

               •  Keep chemical containers  tightly sealed when not in use.

3.     Eating. Smoking, Etc.

               •       Eating,  drinking, smoking, gum chewing, or application of cosmetics  is
                      prohibited in all laboratories.  Wash hands thoroughly before partaking  in
                      these activities.

               •       Storage,  handling, or consumption of  food or beverages in storage areas,
                      refrigerators, glassware, or utensils which are also used for laboratory
                      operations is prohibited.

5.     Equipment and  glassware

               •       Handle and  store laboratory glassware with care to avoid damage; do not
                      use damaged glassware. Use equipment only for its designed purpose.

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6.     Exiting

               •      Wash areas of exposed skin well before leaving the laboratory.

7.     Horseplay

               •      Avoid practical jokes or other behavior  which might  confuse, startle,  or
                      distract another worker.

8.     Mouth suction

               •      Do not use mouth suction for pipetting or siphoning.

9.     Personal Apparel

               •      Confine long hair and loose clothing.  Wear shoes at all times but do not
                      wear sandals or other open shoes.

10.    Personal Housekeeping

               •      Keep  the work area clean and uncluttered, with chemicals and equipment
                      properly labeled and stored.   Keep chemicals away  from  incompatible
                      materials, sunlight, and unnecessary heat or open flames.  Clean up the
                      work  area  on completion  of an operation or at the end of each  day.
                      Return chemicals back to their proper storage place.

11.    Personal Protection

               •      Safety glasses or other appropriate  eye  protection shall  be  worn in the
                      laboratories by all persons including visitors.

               •      Wear  appropriate   gloves  when the  potential  for  contact  with  toxic
                      materials exists.  Ensure that gloves are  free from  contamination before
                      moving from one  procedure  to  the next.   Decontaminate gloves  as
                      necessary.  Inspect  gloves before each use and wash  them before removal.
                      Replace periodically or as needed.

               •      Use other protective and emergency apparel and equipment as needed.

               •      Avoid use of contact lenses  in the laboratory.   If  they are used, the
                      laboratory  supervisor must be  informed  so that  special precautions are
                      taken.

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                      Remove laboratory coats immediately upon significant contamination.  Do
                      not  store lab coats  or  other contaminated personal protective equipment
                      near food,                                         books, desks, clean
                      clothes, etc.
12.     Planning
               •       Review  the MSDS and  Chemical Hygiene  SOP  prior to performing any
                      laboratory  procedure.    Plan  appropriate protective  procedures  and
                      positioning of equipment before beginning any new operation.
13.     Unattended Operations
               •       Leave  lights  on,  place a suitable  sign  on the door,  and provide  for
                      containment  of toxic  substances  in the  event of utility failure to  the
                      operation.
14.     Use of Fume Hoods
                      Use a hood for operations which might result in release of toxic vapors or
                      dust or as required by  the procedure's SOP.  It is good  practice to do a
                      procedure in a fume hood, if practical, even when not required.

                      As a general rule, use a fume  hood when working with volatile substances
                      having a Threshold Limit Value of less than 50 ppm.

                      Keep materials stored in the hood to a minimum and do not  allow them to
                      block  vents or air flow.

                      Carcinogens  and highly  toxic chemicals  must  only  be handled  in
                      designated hoods.

                      Never perform any chemical mixing or vaporization in a heat hood or heat
                      canopy.

                      Leave the hood  "on" when it is not  in active use if toxic substances are
                      stored in it or  if it  is needed to  maintain  general  ventilation (i.e. the
                      Hazardous Materials Storage Locker).

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15.    Working Alone

              •      Avoid working  alone  whenever possible.   Do not work alone if the
                     procedures being conducted are hazardous.

              •      When working alone, have a laboratory staff or crew member occasionally
                     check the work area.

1.6    CHEMICAL HYGIENE STANDARD OPERATING PROCEDURES (SOP)

All laboratory procedures for analysis done on the Lake Guardian can be  found  in the Quality
Assurance Project Plan.  Chemical Hygiene SOP's  for each procedure are bound separately under
the title Chemical Hygiene SOP's for U.S.  EPA's Great Lakes Program Office and  are part of the
Chemical Hygiene Plan.  Further recommendations can be found in a  National Research Council
publication,  1981, entitled Prudent Practices for Handling Hazardous Chemicals in Laboratories.

SOP's  for chemical storage can be found in Attachment 2.  SOP's for Chemical Disposal can be
found  in Attachment  5.   As  lab  procedures vary, these  Chemical  Hygiene SOP's must be
updated.

Depending  on research operations, radioactive materials Ni-63  and C-14 may be  present in the
Analytical and Primary Productivity Laboratories  respectively.   These materials  are  subject to
restriction by the Nuclear Regulatory Commission as  set forth in 10 CFR Part 20.  Guidelines for
safe use  of  these materials on board the Lake Guardian are set  forth in the GLNPO Standard
Operating Procedures for  Radiation  Safety, located in Attachment 6  of this Appendix, and are
administered by the ship's Radiation  Safety/Chemical  Hygiene  Officer.

Designated  Areas

Work with select carcinogens, allergens, reproductive and embryotoxins and substances that  have
a high degree of acute and/or  chronic toxicity may  require additional protection for employees.
For the GLNPO, the following  compounds are used  which are select carcinogens under 29  CFR
1910.1450 (b).

                 •     Beryllium
                 •     Formaldehyde

In  addition, the following  chemicals are considered  "extremely poisonous"  although not on the
OSHA select carcinogen  list. These chemicals should also be used exclusively in the Designated
Area.  These chemicals are:

                 •     Carbon 14
                 •     Chloroform
                 •     Hydrazine Sulfate
                 •     Sodium Nitroprusside

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The Designated Area(s) on the Lake Guardian are:

                •    Fume Hood #4, Bio Lab
                      Fume Hood #6, Primary Prod. Lab
                      Fume Hood #9, Extraction Lab
                      Fume Hood #10, Extraction Lab
These fume hoods shall be labeled as Designated Areas.  SOP's for these areas are dependent on
the material and procedure being done.  Different Personal  Protective Equipment (PPE) may be
required  for  different  chemicals  (See the Chemical  Hygiene  SOP  for  each  lab procedure).
General SOP's in using these fume hoods as a Designated Area are as follows:

                 •    Periodically check the air flow of the  fume hood.
                 •    Wear appropriate PPE.
                 •    Avoid inhalation or  skin contact with  these chemicals.
                 •    Decontaminate or dispose of gloves after use.
                 •    Review MSDS's prior to laboratory procedures.
                 •    Excess hazardous material must be treated as
                     hazardous waste.
                 •    Thorough decontamination of the working area, working surfaces, gloves
                     and equipment must be done prior to doing another procedural step.

Required Prior Approvals

Unique circumstances may require special approval as outlined in the Quality Assurance  manual.
Any unusual procedures or circumstances not covered by the  Laboratory Analytical Procedures or
the Chemical Hygiene Standard Operating Procedures, must be approved by the Limnology Group
Leader.
1.7    CHEMICAL PROCUREMENT,  DISTRIBUTION AND HANDLING

Laboratory employees shall  follow the procedures set forth  in  the document entitled  Standard
Operating  Procedures  for  Hazardous  Material  Storage  and  Spill  Prevention  contained  in
Attachment 3.  This Attachment provides information  concerning procurement,  bulk storage,
distribution, and  laboratory  storage.   Attachment 2  contains the chemical inventory for  the
routine surveys conducted on  the ship as well as information necessary to ensure compatible
storage.

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1.8    SIGNS AND LABELS

Prominent signs and labels for the following shall be posted in the laboratory:

               •      Emergency Telephone Numbers - Emergency telephone numbers and radio
                      contact  with the U.S.  Coast  Guard shall  be maintained by  the  ship's
                      Captain.

               •      Container Labeling  -  All  containers of  chemicals,  cleaning material,
                      maintenance  supplies,   etc. throughout  the  facility  shall  have  labels
                      identifying  the contents.   Damaged labels on such  materials shall be
                      replaced.   In addition,  any transfer containers  of such  chemicals and
                      materials shall be appropriately labeled.  This         is   in   accordance
                      with 29 CFR 1910.1200, the OSHA Hazard Communication Standard.

               •      Safety  and  First Aid Equipment  - All safety  showers, eyewash stations,
                      first  aid kits, fire blankets, and fire extinguishers in the facility shall have
                      proper identification markings.

               •      Exit  Signs - All exits shall be marked and the signs properly maintained.

               •      High Risk Areas  -  All chemical  storage  cabinets shall  be  labeled to
                      indicate general category and chemical compatibility.

               •      Fume hoods #4, #6, #9 and #10  must be labeled with "Designated Area"
                      signs indicating  the  use  of carcinogenic, highly toxic and/or radioactive
                      materials in those areas.

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1.9    HAZARD IDENTIFICATION

Purpose

This procedure  outlines  a method  for determining the health hazards  of a particular chemical
which  will be used  by the laboratory.  For any  chemical which is  purchased  from an outside
supplier, the manufacturer's or supplier's Material Safety Data Sheet (MSDS) and label will be
the principal source of hazard information.   For any chemical  produced in the  laboratory, or
produced as a by-product,  it will be assumed to be hazardous  unless,  or until, its identity can be
determined and a specific hazard analysis is performed.

Scope

This procedure  will  cover all hazardous chemicals used  in the laboratory, whether brought  into
the laboratory  from an  outside manufacturer  or  supplier, or produced in the laboratory  as a
product, intermediate or by-product.  This procedure  also details  the acquisition  and maintenance
of MSDS and container labeling.

Procedures

The Chemical Hygiene Officer (CHO)  will review  each new or revised MSDS when it is received
from a manufacturer or supplier.  The review will be conducted to assure that it is complete and
that it provides information  concerning the  health hazards, special  procedures  or practices for
handling and storage,  emergency procedures  and disposal methods.   In conjunction with the
appropriate laboratory supervisor, the CHO will determine if special procedures or practices  need
to be implemented to assure safe handling.

If available, supplier's  samples must include a Material Safety  Data Sheet for the use of operating
personnel in evaluating the product.  The inventory can be found in Attachment 2 of this CHP.

If a Material Safety  Data  Sheet is missing, the CHO  will secure it through appropriate channels.
Suppliers  who  fail  to  cooperate in  providing MSDSs  will be identified to  the appropriate
management personnel  for disposition.

All labels affixed to incoming chemical containers must be maintained.  Contact the CHO if any
container labels are  missing or defaced.  The  container with  a missing or defaced label will be
removed by the  CHO from stock and not returned until the condition has been rectified.

If any laboratory procedure will result  in the production of a by-product which is  unknown, it will
be  considered hazardous and handled according to the SOP for work  with particularly  hazardous
substances.

If any laboratory procedure results in an intermediate  or final  product whose composition is
known, a literature search will be conducted to determine the hazardous nature  of the chemical.
Following  this  hazard  evaluation,  the  CHO, in  conjunction  with  other members of the

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management  staff as  needed will develop appropriate  practices  and procedures to  assure safe
handling, use, storage and disposal of the chemical.   These procedures will be included with the
SOPs for the work with hazardous chemicals to ensure employee protection.

In addition  to the MSDS's,  other information concerning hazards, safe  handling, storage,  and
disposal can be found in the CHO office and  in the central Great Lakes National Program Office,
77 W. Jackson Blvd., Chicago, IL 60604-3590.

Material Safety Data Sheets (MSDS)

Since such a wide variety of substances are  utilized  in our facilities, one central recordkeeping
area will be used for storage of MSDSs for the entire facility.  In this way, everyone will know
the location of MSDS information.  The following procedures  cover recordkeeping and filing of
MSDSs for chemicals used in the laboratories.   Additional copies of MSDSs should be made
available at each Laboratory to facilitate easy access.

               •      Before any chemical is received at the facility, each supplier of hazardous
                      materials  will  be contacted  and  an  MSDS for  their  products  will  be
                      obtained.  This initial request  will  be formalized as part of the purchasing
                      procedure, i.e.  an MSDS will  be requested during the verbal ordering and
                      again on the written purchase  order.

               •      A master file and cross reference list of MSDSs  by trade name, chemical
                      names, supplier and user  will be developed and maintained by the CHO
                      and located in the CHO office.

               •      A file of MSDSs for chemicals used in  each laboratory will be located in
                      the central hallway.

               •      The laboratory  file of MSDSs should be  used  for  employee  training
                      purposes and must be  available for  employee access.

               •      The CHO will  make MSDSs  available to any outside medical  provider or
                      public service agency that  needs the information.

               •      MSDSs for products that are not currently used or  that  have been changed
                      will be maintained in an inactive file for a minimum of 30 years in the lab
                      office on board the Lake Guardian, then in a central GLNPO Office.

We  will  rely exclusively on the information supplied by the product vendor or distributor of the
Material Safety  Data Sheet to properly determine the hazards of any particular chemical used in
the laboratories.  MSDS's that are incomplete or appear to be in error will be referred back to the
vendor or  distributor by the CHO  for information or  change.   If  you notice any missing
information or information which appears to be inaccurate, please contact the CHO.

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Note: MSDSs should be maintained in accordance with 1910.20 subpart C - General Safety and
Health  Provisions  -  "Access  to  Employee Exposure and  Medical  Records."   Paragraph  (d)
Preservation of Records; (1) (11)  (A)  & (B), Material  Safety Data Sheets; and paragraph (c)  (5)
(iv).  Records concerning the identity of a substance or agent need not be retained for any specific
period,  as long as some record of the identity (chemical name  if known) of the substance or
agent, where it is used, and when it was used are retained for at least thirty (30) years.

Labels

Labels  are  designed  to provide information to  employees concerning  the  hazards  of various
chemicals.   Therefore,  it  is  important that hazardous  chemicals remain  in properly labeled
containers.  The following procedures apply to all manufacturers' labels.

        •       All manufacturers' labels will remain on  the containers.  If any container  labels
               are missing or defaced, please contact your supervisor.

        •       As a minimum, each label should contain the following:

               1.  Identification of the material in the container;

               2.  Appropriate hazard warnings,  such as health, fire, and reactivity;

               3.  Name and address of chemical manufacturer, importer, or the responsible party
                  (applies only to manufacturers' labels).

        •       If you are unfamiliar with the contents on the label, please see the lab supervisor
               for further explanation.

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In laboratory storage, reaction or processing containers should be labeled with:

       •       Identification of material in the container, such as, product or chemical name

       •       Whenever possible, appropriate  hazard warnings as lo health, fire, or reactivity
               taken from the  Material  Safety  Data  Sheets,  or  other  hazard  identification
               reference.

       •       Containers  of chemicals produced in-house will be labeled with appropriate hazard
               information by the CHO.

1.10   INDUSTRIAL HYGIENE

Purpose

Industrial  Hygiene is the science of recognition, evaluation, and control of environmental health
hazards arising in the work place.   This  procedure describes  how chemical  hazards in the
laboratory  will be evaluated.   Material Safety Data Sheets  specify the health hazards associated
with  a hazardous material.    A  professional  Industrial  Hygienist is needed to interpret  this
information,  especially  Permissible  Exposure  Limits  (PEL's)  and  Threshold  Limit Values
(TLV's), in relationship to  how hazardous materials are used in the laboratory environment.
Scope

Certain hazardous chemicals  require an industrial hygiene study to determine  the  level of the
hazardous chemical and extent of control needed to protect the employee.  These efforts will be
coordinated by  the  Chemical Hygiene  Officer  (CHO).   Where  a question exists  concerning
employee exposure to hazardous materials, engineering controls, or personal protection equipment
requirements,  the CHO should be contacted immediately.
Laboratory Procedures

Employee  Exposure Determination   In many of the  laboratory procedures, multiple hazardous
chemicals are used, however, the quantities are small and the duration of use is short. For these
reasons, monitoring  of the  potential  employee  exposure due to airborne chemicals  will  be
conducted  if there is  sufficient reason to believe  that an exposure is possible.   The following
criteria will be used to determine if monitoring will be conducted:
                                          •*e>
               The toxicity and volatility of the hazardous chemical in use.  If a highly toxic or
               highly volatile material is handled during a procedure,  these operations will be
               placed on a priority evaluation list to determine if sampling is needed.

               The manner in which the laboratory procedure is  conducted.  Procedures  which

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              must be conducted outside a lab hood or unventilated area, if toxic chemicals must
              be heated, procedures must be conducted in open vessels vs. closed containers.

       •      Quantity of, and frequency at which a hazardous chemical is used in the lab.

       •      Report of an employee experiencing signs or symptoms of exposure, such as, skin
              or eye irritation, shortness of breath, headache, etc.

As  required  by the  OSHA Lab  standard,  29 CFR  1910.1450  (See  Attachment  1 of this
Appendix), monitoring will be conducted if the above conditions could result in an employee over
exposure during use of the following chemicals regulated by OSHA.

       •      OSHA Regulated Substances, according to 29 CFR 1910 Subpart Z, dated July 1,
              1992, are the following:
              Asbestos
              Coal Tar Pitch Volatiles
              4-Nitrobiphenyl
              alpha-Naphthylamine
              Methyl-chloromethyl ether
              3, 3 -Dichlorobenzidine and its salts
              bis-Chloromethyl ether
              beta-Naphthylamine
              Benzidine
              4-Aminodiphenyl
              Ethyleneimine
              beta-Propiolactone
              2-Acetylaminofluorene
              4-Dimethylaminoazobenzene
              N-Nitrosodimethylamine
              Vinyl chloride
              Inorganic Arsenic
              Lead
              Benzene
              Coke Oven Emissions
              Bloodborne Pathogens
              Cotton Dust
              1, 2-dibromo-3-chloropropane
              Acrylonitrile
              Ethylene oxide
              Formaldehyde
              Methylenedianiline

Monitoring of employee  exposure  may also be conducted for any  other chemicals used in the
laboratory if the conditions stated under paragraph 1 are experienced.

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The  CHO  will coordinate employee monitoring.  The CHO  should be contacted  if the above
conditions  are  observed or if plans for a new procedure or  use of a new chemical could be
expected to create any of the conditions described above.

Personal Protective Equipment (PPE)

Selection,  use,  maintenance and  storage  of all  respiratory  protective equipment will  be  in
accordance with the Respiratory Protection Program, as stated in Appendix A of this manual.

Safety glasses  will  be  worn  at  all times when  working in any  laboratory  where hazardous
chemicals are used.  Additional  requirements for eye protection, such as chemical splash  guard,
goggles, full face respirators, etc., will be determined by  the laboratory SOP for use of that
chemical.

Protective clothing requirements, such as,  lab coats,  protective  gloves, coveralls,  etc., will be
determined by the laboratory SOP for use of that chemical.  Determinations are based on  MSDS
Recommendations and  "Guidelines for the  Selection  of Chemical  Protective Clothing", available
from the American  Conference of Governmental Industrial Hygienists, 6500 Glenway  Drive,
Cincinnati, OH, 45211,  (513)661-7881.

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Preventive Maintenance Programs

Exhaust Ventilation:  The local exhaust ventilation system will be maintained on a quarterly basis
by  the  Maintenance  Department/Engineering Department,  with the  assistance of the Chemical
Hygiene Officer. The evaluation will include:

        •  Hood performance

         - Use smoke tubes to visualize  the  air  flow  patterns within the  hood.    Excessive
           turbulence,  uneven exhaust air flow patterns, effects of make-up  air will be evident
           from the smoke tube evaluation.

         - Measure the air velocity with a  calibrated velometer.  Make a series of measurement
           to assure  the even distribution of the exhausted  air stream.  Air velocities should  not
           vary more than 20% -25% over the face of the hood.

         - Always perform  this evaluation  with the  hood sash in  the  full  open position.   If
           adequate velocities are not attainable at the full open position, remove the hood from
           service until it can be maintained.

        •  Exhaust fan.

         - Maintain the exhaust fan, e.g. drive belts, lubrication, etc.

         - Observe proper direction of the fan rotation.

         - Determine the condition of the fan blades.

         - Check the performance  of multi-speed  fans, solenoids,  etc., which  can affect  the
           performance of the hood under different operating conditions.

        •   Additions to the Local Exhaust Ventilation (LEV) system.

Whenever an  additional hood,  or other  ventilated equipment  is added  to the system, the entire
system should be re-evaluated.  Following the evaluation, necessary repairs or additions should be
made to the system.

Eyewashes and  Safety Showers:   Eyewashes and Safety Showers will  be checked on a weekly
basis by the CHO in the laboratory.

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1.11   EMPLOYEE INFORMATION AND TRAINING
Purpose

This procedure outlines the laboratory's policy for training employees who are required to handle
hazardous chemicals.
Scope

The  Information and Training Program will teach employees about the hazards of the chemicals
used in their areas  and the protective measures by  which they can protect themselves from
exposure to  these chemicals.   At a  minimum,  the  following areas  will be covered  either  in
training sessions or provided as reference materials to which each employee has ready access:

       •   The  contents  of  the  Federal  Standard,  "Occupational  Exposure  to  Hazardous
           Chemicals in Laboratories".

       •   The laboratory's Chemical Hygiene Program and its location.

       •   Permissible  Exposure Limits for chemicals regulated by OSHA and other established
           exposure limits which will be used during employee exposure determinations.

       •   Symptoms associated with overexposure to hazardous materials.

       •   Hazards of chemicals used in the laboratories.  This will be accomplished by teaching
           the employee to read MSDS's and manufacturers' labels.

       •   For  additional  information on  the hazards  of chemicals,  the employees  will have
           access to appropriate reference material.

       •   Use of personal protective equipment.

       •   Standard operating procedures to be implemented when hazardous chemicals are used.

        •   Special procedures that workers involved in use of particularly hazardous  substances
           must follow.

        •  Emergency procedures.

        •  Storage practices.

        •  Waste disposal.

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Procedures

Training Program:  The training program will be presented in three parts.

1.      Part  I  will present an  explanation  of  the  OSHA regulation, the company's Chemical
        Hygiene  Plan  and  where  this  and  other  reference  materials  containing  additional
        information on the hazards, safe handling,  storage and  disposal of hazardous chemicals
        are located.

2.      Part II will include training in basic concepts associated with chemical hazards.   Areas
        covered are:

        a.  Chemical  Hazards - The basic concepts  related to chemical hazards:  Definition of a
           hazard, types of hazards,  exposure  or  dose,  routes of entry, acute versus  chronic
           effects, Threshold Limit Values, and types of control measures.

        b.  Material Safety Data Sheets - This explains what a Material Safety Data Sheet is, how
           it can be used, and what information it contains.

        c.  Labeling,  Storage, and Handling - This introduces types of labels and their purposes,
           emphasizing  the  importance  of reading  and  heeding  labels.    General  storage
           considerations  are  covered  and safe handling  procedures  and  personal  protective
           equipment are discussed.  The intent is to lay the groundwork for a discussion  of the
           specific handling procedures to be used in the laboratory.   This  section stresses the
           importance of following the standard operating procedures.

3.      Part III of the training will include the specific chemical hazards, protective  practices and
        equipment, applicable parts of the  laboratory's  Chemical Hygiene  Plan and standard
        operating procedures for work  with hazardous chemicals in the laboratory  to  which the
        employee is assigned.  Areas covered are:

        a.  Chemicals You Use  -  This will teach  employees about  the chemical hazards and
           control procedures for the  specific chemical hazards  identified for each procedure in
           the laboratory that involves  hazardous chemicals.

        b.  Chemical  Hygiene Plan - A detailed explanation of the CHP will be provided  to the
           employee  including all applicable parts of the CHP.

        c.  Standard Operating Procedures -  Review in detail the SOP's specific to the  laboratory
           where the employee works.

4.      Training will  be provided as follows:

        a.  Newly hired personnel will be trained by  the  CHO before that employee works with
           hazardous chemicals.

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                                                         SECTION:  APPENDIX J
                                                         VERSION:  FINAL/MAY 1997
                                                         PAGE 24 of 29
       b.  Employees  transferred to new positions will be trained by the CHO  before working
           with hazardous chemicals.

       c.  Supplemental training, (i.e., when new chemical hazards or procedures are introduced
           into the laboratory) will be presented to the employee affected by the CHO.

       d.  Refresher training will be provided as deemed necessary by  the CHO.   An  annual
           refresher in selected aspects of chemical hazards is desirable.

5.      All training will be documented:

       a.  The CHO will maintain a list of employees who have received training.

       b.  The  CHO   will  obtain signatures  from  employees  receiving  training,  including
           transferred employees.

1.12   MEDICAL EVALUATION PROGRAM

Medical evaluation and surveillance for EPA  personnel working in the Lake Guardian laboratories
shall be provided by their local office.  All medical services for contractor laboratory employees
shall be provided by their employer.

1.13   PERSONAL PROTECTIVE EQUIPMENT  (PPE)

Personal Protective Equipment  (PPE) shall be  made  available  to  all  laboratory employees.
Appropriate PPE is dependent  on the lab procedure being performed and the chemicals used.
Proper  PPE  is  discussed in  the  Chemical  Hygiene  SOPs  and  Material Safety Data  Sheets
(MSDSs).  Recommendations regarding PPE  shall be based on the "Guidelines for the Selection
of Chemical Protective  Clothing".

Safety showers and eyewash stations shall be conveniently located to laboratories.   These stations
shall be checked weekly. Records of these inspections shall be kept in the CHO's office.

Respiratory protection shall be provided as needed  for Lake Guardian laboratory personnel.

Eye Protection

Appropriate  eye  protection must be  worn in  the  laboratory or when  doing  laboratory  or
mechanical work.  Protective eyewear should never be shared between employees.  Primary types
of eye protection are as follows:

Safety Glasses

All lab  employees who do not wear prescription  eyewear shall have sturdy safety glasses fitted
with  side shields.  These glasses  must be worn  in  the laboratory at all  times,  even when the

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                                                           SECTION: APPENDIX J
                                                           VERSION: FINAL/MAY 1997
                                                           PAGE 25 of 29
employee is  not performing  analytical  work.   This requirement  protects the employee from
hazards generated by  other  analysts'  work.   Persons needing prescription eyewear shall have
safety-rated lenses with side shields, as specified by ANSI Z.87.

Safety Goggles

Safety glasses are not  always  sufficient to protect employees from all  chemical hazards during
analytical procedures.   Safety goggles are  designed to protect the eyes from splash hazards.  All
employees shall wear goggles when performing analytical procedures involving liquids which may
present such a hazard.

Face Shields

Face  shields shall  be  provided  when working with  corrosive materials  or chemicals  that  are
potentially explosive.   Face  shields are  designed to be worn comfortably over safety glasses or
goggles and should not be used in place of these items.  The employee should never use a face
shield in lieu of a protective  barrier designed for working with explosive procedures, but in
conjunction with the barrier.

Gloves

The skin is a potential route of exposure to hazardous  materials on the Lake Guardian.  It is very
important  that  gloves  are worn when  working with chemicals  or performing  operations that
involve heat hazards.

The analyst should not assume that a pair  of gloves will provide adequate protection, even when
they are  new.  Gloves should be examined for discoloration  and punctures before each use.  The
employee should verify that the gloves are appropriate for the hazard.  Sometimes,  when working
with mixtures,  double or  even  triple gloving may be necessary.  Following is  a brief description
of the type of glove that  should be worn  for some laboratory operations or when working with
specific chemicals.   In addition, the OHSD also provides  a manual  on the proper  selection of
personal  protective equipment that may be consulted if the hazard is not listed here.

Hot Objects                   Fiberglass or treated leather gloves; never wear asbestos gloves

Acids (HC1, Nitric)            Natural Rubber

Acids (H2SO4)                 Neoprene

Bases                         Natural Rubber

PCBs                         Neoprene or poly vinyl alcohol

Amines                       Butyl Rubber

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                                                         SECTION: APPENDIX J
                                                         VERSION: FINAL/MAY  1997
                                                         PAGE 26 of 29
Ethers                       Polyvinyl Alcohol

Xylene                       Polyvinyl alcohol

Methylene Chloride           Vitron/Neoprene

Pentachlorophenol             Nitrile

Mercury                     Poly vinyl alcohol

Carbon Disulfide              Poly vinyl alcohol

Phenol                       Butyl rubber

2-Nitro Fluorine*             Butyl Rubber/Nitrile

t-Butyl Alcohol*              Butyl Rubber/Nitrile
*Data currently does not exist relating to appropriate glove type.  The gloves were
recommended by a manufacturer.
1.14   RECORDKEEPING

This section should meet the requirements of 1910.1450 (j)  and be consistent  with  EPA Order
1440   "Occupational  Health  and  Safety  Manual,"  CHAPTER   3   ("Accident  and  Illness
Investigation, Reporting and Recordkeeping Requirements"), 29 CER  1960 Subpart I, 29 CFR
1910.20 and relevant sections of other OSHA standards.

The following records shall be kept on board the Lake Guardian  and  will be managed by the
Chemical Hygiene Officer:

       1.  Accident / Illness records;

       2.  CHP records - compatible with current knowledge and regulations, employee training,
           MSDSs, current chemical inventory;

       3.  Inventory and use records for hazardous and high-risk substances;

       4.  Respiratory fit testing records.

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                                                          SECTION:  APPENDIX J
                                                          VERSION:  FINAL/MAY 1997
                                                          PAGE 27 of 29
1.15   HOUSEKEEPING, INSPECTION AND MAINTENANCE

Housekeeping -  Floors  shall be cleaned before and after each survey.  Floors shall be cleaned
during a survey, as needed, to maintain dry conditions free of chemical contamination.

Storage of laboratory equipment  must not block hallways  or exits or obstruct access  to  safety
showers, eyewashes,  spill kits, first aid supplies, and fire equipment.

Laboratory equipment and supplies must be used and stored in a secure manner always bearing in
mind the ship's movement during rough weather.

Inspection - The CHO shall conduct weekly inspections of the laboratories,  general ship safety,
and the hazardous  materials storage area.  Copies of the current checksheets used for inspection
shall be maintained in Attachment 4.
The safety showers and the GFCI outlets shall be inspected before the beginning of each survey.
Safety showers will be inspected weekly thereafter.

Maintenance  - After  inspections,  arrangements  shall  be made with  the  Chief Engineer  or
Electrician for any  needed repairs.  Those repairs which cannot  be made immediately should be
reported to the EPA Region V Health and Safety Officer for inclusion  in the next work list for the
ship.

Laboratory personnel should  report any problems or shortages associated with safety and health
equipment to the CHO.

1.16   WASTE DISPOSAL

Waste disposal for  the Lake Guardian  laboratories shall be conducted according to the Standard
Operating Procedures for Hazardous and Common Waste Disposal contained in Attachment 5.
1.17   SPILLS AND ACCIDENTS

The  best  remedy for spills and accidents  is to prevent their  occurrence.  Guidelines for the
prevention of chemical spills can be found  in Attachment 3,  the Standard Operating Procedures
for Hazardous Material  Storage and Spill Prevention.  Strict adherence to all standard operating
procedures and the guidelines set  forth in this Chemical  Hygiene Plan will greatly reduce the
chance for spills and accidents in the Lake Guardian laboratories.

Spills that do  occur shall  be remediated following  the  guidelines set  forth in the R/V Lake
Guardian  Hazardous Materials / Hazardous  Waste Contingency Plan, as stated in Appendix R of
this manual.

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                                                          SECTION:  APPENDIX J
                                                          VERSION:  FINAL/MAY 1997
                                                          PAGE 28 of 29
The following guidelines shall be used in responding to accidents which may occur:

General Emergency Procedures - General Emergency Procedures for the operation of the boat can
be found in Research Vessel Safety Procedures & Seaward Services, Inc. Occupational Safety &
Health Manual, found in Appendix N of this manual.
Chemical Emergency Procedures

•   Be aware of antidote or dilution procedures for all chemicals being used in the procedure.

•   Get victim to deluge shower or eyewash if needed.

•   Contact the bridge and/or the CHO. Contact Captain for emergency phone numbers.

•   The Captain shall notify the proper authorities and summon help as needed.

General First Aid -  First  aid supplies are maintained  in portable kits throughout the ship.
Additionally, there is a major supply station in the Captain's office.

Eye Contact:  Promptly flush eyes with water for a period of at least 15 minutes and seek medical
attention.

Ingestion:  Follow the directives  in the chemical's MSDS and  take appropriate  action.  Seek
medical attention.

Skin Contact:  Remove all contaminated clothing and wash the  affected area thoroughly  at the
sink or in the emergency deluge shower.  Seek medical attention as needed.

1.18     SAFETY PROCEDURES

To ensure the health and safety of those working in the Lake Guardian laboratories, the following
safety  procedures shall be observed in  addition  to  those  procedures  directly  involved with
chemical hygiene:

1.  Compressed Gas  Cylinders - All compressed gas cylinders  must be  secured to  permanent
    cylinder  storage fixtures  at all times.   Appropriate  fixtures are located in  the  Analytical
    Laboratory and on the 01 deck.  Small capacity cylinders which cannot be  secured  to the
    fixtures shall be secured by some other means  in such a way as to prevent rolling,  falling, or
    bumping of  the cylinders.   Cylinders containing  oxygen shall be  stored  separately from
    cylinders and  other materials having highly  flammable constituents by a distance of 20 feet or
    a half hour  fire wall.  This does not apply to oxygen cylinders in use or connected for use.
    As a general  rule,  no more than six compressed gas cylinders  are allowed in a sprinklered
    area, and no more than three are allowed in a non-sprinklered area.  Also, gang chaining of
    compressed gas cylinders is strictly forbidden.

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                                                   SECTION:  APPENDIX J
                                                   VERSION:  FINAL/MAY  1997
                                                   PAGE 29 of 29
2.  Fire  Safety  and  Emergency  Response  Procedures  -  Dry  chemical  (ABC)  fire
    extinguishers shall be located in each of the permanent laboratories  as well as the
    hallway.  Container laboratories shall have their own automatic dry chemical system.
    All laboratory personnel shall  be familiarized  with the  use of immersion  suits, life
    jackets,  and the ship's  station  bill.   When the fire alarm  is sounded all laboratory
    personnel  shall get an  immersion suit and/or  life  jacket and  report  to the location
    assigned  to them  on the  station bill.   Other than for first response  with a fire
    extinguisher, no non-crew laboratory personnel  shall be involved in fire fighting.  Fire
    doors shall be kept closed at all times.

3.  On Deck Safety Procedures - Work vests shall be worn at  all  times during sampling
    and testing conducted on the rosette deck and fantail.   No one is permitted on the
    fantail during sea operations unless directly involved with sampling, testing, checking
    of equipment, or other  work.   No one is permitted to work alone on the fantail.  A
    crew member with a radio should  be  present during work at a sampling  station.
    During weather conditions  causing freezing of water on deck surfaces, hoses shall be
    used to  thaw surfaces with warm water as needed  to prevent a slipping hazard for
    those working outside.

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                                           SECTION:  APPENDIX J, ATTACHMENT
                                           VERSION:  FINAL/MAY 1997
                                           Attachment  I - Cover
ATTACHMENT 1-29 CFR 1910.1450, Revised July 1, 1992

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Labor
29
PART 1910 (§ 1910.1000 TO END)
Revised as of July 1, 1992
                            I

-------
?n)     Occupational Safety and Health Admin., Labor
                                                                 §1910.1450
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        made with the aid of the secret may be in-
        appropriate.

        [52 FR 31877. Aug. 24, 1987, asCamended at
        52 FR 46080, Dec. 4. 1987; 53 FR 15035. Apr.
        27. 1988;  54 FR 24334. June 7, 1989; 54  FR
          B, Feb. 15, 1989]
  91910.1450  Occupational exposure to haz-
    ardous chemicals in laboratories.
   (a) Scope and application. (1)  This
  section shall apply to all employers en-
  gaged in the laboratory use of hazard-
  ous chemicals as defined below.
   (2) Where  this section  applies, it
  shall supersede, for laboratories, the
  requirements  of  all  other  OSHA
  health standards in 29 CFR part  1910,
  subpart Z, except as follows:
   (i) For any OSHA health standard,
  only the requirement to limit employ-
  ee exposure to the specific permissible
  exposure  limit shall  apply-for labora-
  tories, unless that particular  standard
  states  otherwise or unless  the condi-
  tions of paragraph (a)(2)(iii) of  this
 section apply.
  (ii) Prohibition of eye and skin  con-
 tact where  specified by any OSHA
 health standard shall be observed.
  (iii) Where the action level1 (|or in the
 absence of an action level, the permis-
 sible exposure  limit) is routinely ex-
 ceeded for  an  OSHA regulated  sub-
 stance  with exposure monitoring  and
 medical   surveillance  requirements,
 paragraphs  (d)  and  (g)(l)(ii) of  this
 section shall apply.
  (3) This section shall not apply to:
  (i) Uses  of   hazardous  chemicals
 which  do  not meet the definition of
 laboratory use,  and in such cases, the
 employer shall  comply  with the rele-
 vant standard in 29  CFR part 1910,
 subpart Z, even if such use occurs  in a
 laboratory.
  (ii) Laboratory  uses  of  hazardous
 chemicals  which  provide no potential
 for  employee exposure. Examples of
 such conditions might include:
  (A) Procedures  using chemically-im-
 pregnated  test media such as Dip-and-
 Read tests  where a  reagent  strip is
 dipped  into  the specimen to be tested
 and the  results  are interpreted  by
 comparing the color reaction to a color
chart supplied by the manufacturer of
 the test strip; and
  (B) Commercially prepared kits such
as those used in performing pregnancy
  tests  in  which  all  of the  reagents
  needed to conduct the test  are con-
  tained in the kit.
   (b) Definitions—
   Action  level means a concentration
  designated in 29 CFR part 1910 for a
  specific  substance, calculated  as  an
  eight  (8)-hour time-weighted average,
  which initiates certain required activi-
  ties such as  exposure monitoring and
  medical surveillance.
   Assistant  Secretary means  the As-
  sistant Secretary of Labor for Occupa-
  tional Safety and Health, U.S. Depart-
  ment of Labor, or designee.
   Carcinogen (see select carcinogen).
   Chemical Hygiene Officer means an
  employee who is designated by the em-
  ployer, and who  is qualified by train-
  ing or experience, to provide technical
  guidance  in the development  and im-
  plementation of the provisions of the
  Chemical Hygiene Plan.  This defini-
  tion is not intended to place limita-
  tions on the position description or job
  classification that the designated ind-
 vidual shall hold within the employ-
 er's organizational structure.
   Chemical  Hygiene  Plan means  a
 written program developed and imple-
 mented by the employer  which sets
 forth procedures, equipment, personal
 protective equipment and  work prac-
 tices that (i) are capable of protecting
 employees from  the  health hazards
 presented  by  hazardous   chemicals
 used in that particular workplace and
 (ii)  meets the requirements of  para-
 graph (e) of this section.
   Combustible liquid means any liquid
-having a flashpoint at or above 100 °F
 (37.8 °C),  but below 200 "F (93.3 °C),
 except any  mixture  having  compo-
 nents with flashpoints of 200 °F (93.3
 °C), or higher, the total  volume of
 which make up 99 percent  or more of
 the total volume of the mixture.
   Compressed gas means:
  (i) A gas or mixture  of gases having,
 in a  container, an  absolute pressure
 exceeding  40 psi at  70 *F (21.1 *C); or
  (ii) A gas or mixture of gases having.
 in a  container, an  absolute pressurfc
 exceeding 104 psi at 130 °F (54.4 °C) re-
 gardless of the pressure at 70 °F (21.1
 °C); or
  (iii) A liquid having a vapor pressure
exceeding 40 psi at 100 °F (37.8 °C) as
determined by ASTM D-323-72.
                                         377

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 §1910.1450
       29 CFR Ch. XVII (7-1-92 Edition)
   Designated  area  means  an  area
 which may  be  used  for  work  with
 "select   carcinogens,"  reproductive
 toxins or  substances  which have  a
 hfgh degree of acute toxicity. A desig-
 nated area may be the entire laborato-
 ry, an area of a laboratory, or a device
 such as i laboratory hood.
   Emergency  means  any  occurrence
 such as, but not limited to, equipment
 failure, rupture  of containers or fail-
 ure of control equipment which re-
 sults  in an uncontrolled release of  a
 hazardous  chemical into  the  work-
 place.
   Employee means  an individual em-
 ployed in a laboratory workplace who
 may  be exposed to hazardous chemi-
 cals in the course of his or her assign-
 ments.
   Explosive  means a  chemical  that
 causes a sudden, almost instantaneous
 release  of  pressure,  gas,  and  heat
 when subjected to sudden shock, pres-
 sure, or high temperature.
   Flammable means a chemical that
 falls into one of  the following catego-
 ries:
   (i) Aerosol, flammable means an aer-
 osol that, when tested  by the method
 described in 16 CFR 1500.45, yields a
 flame  protection exceeding 18 inches
 at full valve opening, or a flashback (a
 flame  extending back to the valve) at
 any degree of valve opening;
  (ii) Gas, flammable means:
  (A) A gas that, at ambient tempera-
 ture and pressure, forms a  flammable
 mixture with air at a concentration of
 13 percent by volume or less; or
  (B) A gas that, at ambient tempera-
 ture and  pressure, forms  a range of
 flammable  mixtures with  air wider
 than 12 percent by volume, regardless
 of the lower limit.         :
  (iii)  Liquid,  flammable means  any
 liquid having a flashpoint below 100 °F
 (37.8 °C), except any mixture having
 components with flashpoints of 100 °F
 (37.8 °C) or  higher, the total of which
 make up  99 percent or more of the
 total volume of the mixture.
  (iv) Solid, flammable  means a solid,
 other than a blasting agent or explo-
sive as defined in § 1910.109(a), that is
 liable to cause fire through  friction,
 absorption of  moisture, spontaneous
 chemical  change,  or   retained  heat
 frnm manufacturing or nrocessine. or
 which can be ignited readily and when
 ignited  burns so vigorously  and per-
 sistently as to create a serious hazard.
 A chemical shall be considered to be a
 flammable solid if, when tested by the
 method described in 16 CFR 1500.44, it
 ignites and burns with a self-sustained
 flame at a rate greater than one-tenth
 of an inch per second  along its major
 axis.
   Flashpoint means the minimum tem-
 perature at which a liquid gives off a
 vapor in sufficient concentration  to
 ignite when tested as follows:
   (i) Tagliabue Closed  Tester  (See
 American National Standard Method
 of Test for Flash Point by Tag Closed
 Tester, Zll.24-1979 (ASTM D 56-79))-
 for liquids; with a viscosity of less than
 45 Saybolt Universal Seconds (SUS) at
 100 *F (37.8 'O, that  do not contain
 suspended solids and  do not  have a
 tendency to form a surface film under
 test; or
   (ii) Pensky-Martens  Closed Tester
 (see  American  National  Standard
 Method  of Test for Flash Point by
 Pensky-Martens  Closed Tester, Z11.7-
 1979 (ASTM D 93-79))-for liquids with
 a  viscosity equal to or greater than 45
 SUS at 100 'F (37.8 °C), or that contain
 suspended solids, or that have a tend-
 ency to form a surface film under test;  ,
 or
  (iii)  Setaflash Closed  Tester (see
 American  National Standard  Method
 of Test for Flash Point by Setaflash
 Closed Tester (ASTM D 3278-78)).
  Organic  peroxides, which undergo
 autoaccelerating thermal  decomposi-
 tion,  are excluded from any  of  the
 flashpoint  determination   methods
 specified above.
  Hazardous chemical means a chemi-
 cal for which there is statistically si>
 nificant evidence based on at least ont
 study conducted in accordance with
 established scientific principles that
 acute or  chronic health  effects may
 occur in  exposed employees. The term
 "health hazard" includes  chemical*
 which are carcinogens, toxic or highly
 toxic agents, reproductive toxins, im-
 tants, corrosives, sensitizers,  hepato
 toxins,   nephrotoxins,   neurotoxuu,
 agents which act on the hematopoietit
systems, and agents which damage iht
lungs,  skin,  eyes,  or  mucous mem-
branes.

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a
o
   Appendices A and B of the Hazard
  Communication  Standard  (29  CFR
  1910.1200) provide  further guidance in
  defining the scope of health  hazards
  and  determining  whether (or' not  a
  chemical is to be considered hazardous
  for purposes of this standard.
   Laboratory means a facility where
  the  "laboratory   use  of  hazardous
  chemicals" occurs.  It is  a workplace
  where  relatively small  quantities  of
  hazardous  chemicals  are used  on  a
  non-production basis.
   Laboratory scale means work with
  substances  in  which the  containers
  used for reactions, transfers, and other
  handling of substances are designed to
  be easily and safely manipulated by
  one person. "Laboratory scale"  ex-
  cludes those workplaces  whose func-
  tion is to produce  commercial  quanti-
  ties of materials.
   Laboratory-type hood means a device
  located in  a laboratory, enclosure on
  five sides  with a  moveable sash or
  fixed partial enclosed on the remain-
 ing side; constructed and maintained
 to draw air from the laboratory and to
 prevent or minimize the escape of  air
 contaminants into the laboratory; and
 allows chemical manipulations to  be
 :onducted in the enclosure without in-
 sertion of any portion of  the employ-
 ee's body other than hands and arms.
  Walk-in   hoods   with   adjustable.
 sashes meet the above definition pro-
 vided that  the  sashes are, adjusted
 during use so that the airflow and the
 exhaust of  air contaminants are not
 compromised and -employees do not
 work inside the  enclosure during the
 release of airborne hazardous  chemi-
 cals.
  Laboratory use of hazardous  chemi-
 cals means  handling or  use of such
 chemicals in which all of the following
 conditions are met:
  (i) Chemical manipulations are  car-
 ried out on a "laboratory scale;"
  (ii) Multiple chemical procedures  or
 chemicals are used;
  (iii) The procedures involved are not
 part of a production process, nor  in
 any way  simulate a production proc-
ess; and
  (iv) "Protective laboratory practices
and equipment" are available; and in
 ;ommon use to minimize the potential
  for employee  exposure to hazardous
  chemicals.
    Medical consultation means  a con-
  sultation which takes place between
  an employee and a licensed physician
  for the  purpose of determining what
  medical  examinations or procedures, if
  any, are appropriate in cases where a
 .significant  exposure  to  a hazardous
  chemical may have taken place.
    Organic, peroxide means  an organic
  compound that contains the bivalent
  — O-O—structure  and which may be
  considered to  be  a structural deriva-
  tive of hydrogen peroxide  where one
  or both  of the hydrogen  atoms has
  been replaced by an organic radical.
    Oxidizer means  a  chemical other
  than, a blasting agent or .explosive as
  defined  in § 1910.109(a), that initiates
  or promotes combustion in other ma-
  terials, thereby causing fire either of
  itself or  through the release of oxygen
  or other  gases.
   Physical hazard  means  a chemical
  for which  there fe scientifically  valid
 evidence   that   it  is  a  combustible
 liquid, a compressed  gas,  explosive,
 flammable, an organic peroxide, an ox-
 idizer, pyrophoric,  unstable (reactive)
 or water-reactive.
-  -Protective laboratory practices and
 equipment -means  those  laboratory
 procedures,  practices and equipment
 accepted  by  laboratory  health  and
 safety experts as effective, or that the
.employer can show to be effective, in
 minimizing the  potential for employee
••exposure  to hazardous chemicals.
  • Reproductive  toxins . means chemi-
 cals which affect the reproductive ca-
pabilities   including   chromosomal
 damage (mutations) and effects on fe-
 tuses (teratogenesis)
  Select carcinogen  means  any  sub-
stance which meets one of the follow-
ing criteria:
  (i) It is  regulated by OSHA as a car-
cinogen; or
  (ii) It is listed under the category,
"known to be  carcinogens,"  in  the
Annual Report  on  Carcinogens  pub-
lished by  the National Toxicology Pro-
gram (NTP) (latest edition); or
  (iii) It is listed under  Group 1 ("car-i^
cinogenic  to humans") by the Interna-
tional Agency for Research on Cancer
Monographs (IARC) (latest  editions);
or
                                      379

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 § 1910.1450

   (iv) It is listed in either Group 2A or
 2B  by  IARC or  under the category,
 "reasonably anticipated to  be carcino-
 gens" by NTP, and causes statistically
 significant tumor incidence in experi-
 mental  animals  in  accordance  with
 aky of the following criteria:
   (A) After inhalation exposure of 6-7
 hours per day, 5 days per week, for a
 significant portion of a lifetime to dos-
 ages of less than 10 mg/m3;
   (B) After  repeated skin application
 of less  than  300  (mg/kg of  body
 weight) per week; or
   (C) After oral dosages of less than 50
 mg/kg of body weight per day.
   Unstable (reactive) means a chemi-
 cal which .is the pure state, or as pro-
 duced or transported,  will  vigorously
 polymerize,  decompose, condense,  or
 will become  self-reactive under  condi-
 tions of shocks, pressure or tempera-
 ture.
   Water-reactive means  a  chemical
 that  reacts with water to release a gas
 that  is either flammable or presents a
 health hazard.
  (c)  Permissible exposure limits. For
 laboratory uses of OSHA regulated
 substances, the  employer shall assure
 that  laboratory employees' exposures
 to such substances do not exceed the
 permissible exposure limits specified
 in 29 CPR part 1910, subpart Z.
  (d)  Employee exposure  determina-
 tion—ID Initial monitoring. The em-
 ployer shall measure  the  employee's
 exposure  to  any substance regulated
 by a standard which requires monitor-
 ing if there  is  reason to believe that
 exposure levels for that substance rou-
 tinely exceed the action level (or in
 the  absence of an  action  level, the
 PEL).
  (2)  Periodic monitoring. If the ini-
 tial  monitoring prescribed by  para
 graph (d)(l) of this section discloses
 employee  exposure over the  action
 level  (or in the absence of an action
 level, the PEL), the employer shall im-
mediately comply  with the exposure
monitoring provisions of the relevant
standard.
  (3) Termination of monitoring. Mon-
itoring may be terminated in accord-
ance with the relevant standard.
  (4)  Employee notification of moni-
toring results.  The  employer  shall,
within 15 working days  after the re-
      29 CFR Ch. XVII (7-1-92 Editi*}

 ceipt of any monitoring results, noui>
 the employee of these results in writ-
 ing either  individually  or by postir4
 results in an appropriate location th*i
 is accessible to employees,
   (e) Chemical hygiene plan—General
 (Appendix  A of  this section is non-
 mandatory  but provides  guidance i&
 assist employers in the development o!
 the  Chemical   Hygiene   Plan.) dj
 Where hazardous chemicals as defined
 by this standard are used in the work-
 place, the employer shall  develop and
 carry out  the provisions of a writu-n
 Chemical Hygiene Plan which is:
  (i) Capable of protecting employees
 from health hazards  associated with
 hazardous chemicals in  that laborato-
 ry and
  (ii) Capable of  keeping exposures
 below the limits specified in paragraph
 (c) of this section.
  (2) The Chemical Hygiene Plan shall
 be  readily  available to employees, em-
 ployee  representatives  and. upon  re-
 quest, to the Assistant Secretary.
  (3) The Chemical Hygiene Plan shall
 include each of the following elements
 and shall  indicate  specific measures
 that the employer  will take to ensure
 laboratory employee protection:
  (i)  Standard  operating  procedures
 relevant to safety and health consider-
 ations to be followed when laboratory
 work  involves the  use  of hazardous
 chemicals;
  (ii)  Criteria that the employer will
 use to determine and implement con-
 trol measures to reduce  employee ex-
 posure to hazardous chemicals includ-
 ing engineering controls,  the use of
 personal protective equipment and hy-
 giene practices;  particular  attention
 shall be given to the selection of con-
 trol measures for chemicals that are
 known to be extremely hazardous;
  (iii) A requirement that fume hoods
 and other protective  equipment are
 functioning  properly    and  specific
 measures that shall be taken to ensure
 proper and  adequate performance of
 such equipment;
  (iv)  Provisions  for employee infor-
 mation  and training as  prescribed in
 paragraph (f) of this section;
  (v) The circumstances under which a
particular laboratory operation, proce-
dure or activity shall require prior ap-
proval from the employer  or  the em-

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  ployer's designee before implementa-
  tion;
   (vi) Provisions for medical consulta-
  tion and medical examinations in ac-
  cordance with  paragraph v
-------
 § 1910.1450
29 CFR Ch. XVII (7-1-92 Edition)
 medical  surveillance  shall  be estab-
 lished for the affected employee  as
 prescribed by  the particular standard.
   (iii) Whenever an event takes place
 in the work area such as a spill, leak,
 explosion or other occurrence result-
 ing in the likelihood of a hazardous
 exposure, the  affected employee shall
 be provided an opportunity for a medi-
 cal consultation.  Such consultation
 sha'l  be for the purpose of  determin-
 ing the need  for a medical examina-
 tion.
   (2)  All  medical  examinations  and
 consultations shall be  performed by or
 under the direct supervision of a li-
 censed physician and shall be provided
 without cost to the employee, without
 loss of pay and at a  reasonable time
 and place.
   (3) Information provided to the phy-
 sician. The employer shall provide the
 following  information to  the  physi-
 cian:
   (i) The  identity  of the hazardous
 chemical(s)  to which the  employee
 may have been exposed;
   (ii) A description of the conditions
 under  which the exposure occurred in-
 cluding quantitative exposure data, if
 available; and
   (iii)  A description of the signs and
 symptoms of exposure  that the em-
 ployee is experiencing,  if any.
   (4) Physician's written opinion, (i)
 For examination or consultation re-
 quired  under this standard,  the em-
 ployer shall  obtain a  written opinion
 from the examining physician which
 shall include  the following:
  (A) Any recommendation for further
 medical follow-up;
  (B) The results of the medical exam-
 ination and any associated tests;
  (C)  Any medical  condition  which
 may be revealed in the course of the
 examination which may place the em-
 ployee  at increased risk as a  result of
 exposure  to  a hazardous  chemical
 found in the workplace; and
  (D) A statement that the employee
 has been informed by the physician of
 the results of the consultation or med-
 ical examination and any medical con-
 dition that may require further exami-
nation  or treatment.
  (ii) The  written opinion shall not
reveal  specific  findings  of diagnoses
unrelated to occupational exposure.
   (h) Hazard identification. (1) With
 respect to labels and material safet
 data sheets:
   (i)  Employers  shall  ensure  tha
 labels on incoming containers of haz-
 ardous chemicals are not removed or
 defaced.
   (ii)  Employers shall  maintain any
 material safety data sheets that  are
 received  with incoming shipments of
 hazardous chemicals, and ensure that
 they are readily accessible to laborato-
 ry employees.
   (2)  The following  provisions shall
 apply  to  chemical substances devel-
 oped in the laboratory:
   (i) If the composition of the chemi-
 cal substance which is produced exclu-
 sively  for  the laboratory's  use  is
 known, the  employer shall determine
 if it is a hazardous chemical as defined
 in paragraph (b) of this section. If the
 chemical is  determined to be hazard-
 ous, the employer shall provide appro-
 priate training as required under para-
 graph (f) of this section.
   (ii) If the chemical produced is a by-
 product  whose  composition  is  not
 known,  the  employer shall
 that  the  substance is hazardous a
 shall implement paragraph (e) of this
 section.
  (iii) If the chemical substance is pro-
 duced for  another user outside of the
 laboratory, the employer shall comply
 with   the   Hazard   Communication
 Standard (29 CPR 1910.1200)  includ-
 ing the requirements  for preparation
 of material safety data sheets and  la-
 beling.
  (i) Use of respirators. Where the use
 of respirators is necessary to  maintain
 exposure  below permissible  exposure
 limits, the employer shall provide,  at
 no cost to the  employee,  the  proper
 respiratory  equipment.  Respirators
 shall  be selected and  used in accord-
 ance with the requirements of 29 CPR
 1910.134.
  (j) Recordkeeping. (1) The employer
 shall establish and maintain  for  each
 employee  an  accurate record of any
 measurements  taken  to  monitor em-
 ployee exposures and any medical con
 sultation and examinations includin
 tests or written opinions  required
 this standard.
  (2) The employer shall  assure  that
such records are kept, transferred, and
                            - » *.
                            i
                          ine
                           i
                                  382

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  made available  in accordance with  29
  CFR 1910.20.
    (k)  Dates—(1)  Effective  date.  This
  section shall become effective  May 1,
  1990.
    (2)  Start-up  dates,  (i)  Employers
  shall have developed and implemented
  a written Chemical  Hygiene Plan no
  later than January 31, 1991.
    (ii) Paragraph (a)(2)  of this'section
  shall not take effect until the employ-
  er has developed and implemented a
  written Chemical  Hygiene Plan.
    (1) Appendices. The information con-
  tained in the appendices is not intend-
  ed, by itself, to create  any additional
  obligations not  otherwise imposed or
  to detract  from  any existing  obliga-
  tion.
  (55 FR 3327, Jan. 31, 1990, 55 FR 7967, Mar.
  6,  1990, 55 FR 12111, Mar. 30, 1990]

  APPENDIX  A  TO § 1910.1450—NATIONAL RE-
   SEARCH  COUNCIL RECOMMENDATIONS  CON-
   CERNING  CHEMICAL HYGIENE IN LABORATO-
   RIES (NON-MANDATORY)

            TABLE OF CONTENTS

                Foreword

 Corresponding Sections of the Standard and
              This Appendix

           A. General Principles '
  1. Minimize all Chemical Exposures
  2. Avoid Underestimation  of Risk
  3. Provide Adequate Ventilation
  4. Institute a Chemical Hygiene Program
  5. Observe the PELs and TLVs

            B. Responsibilities
  1. Chief Executive Officer
  2. Supervisor of Administrative Unit
  3. Chemical Hygiene Officer
  4. Laboratory Supervisor
  5. Project Director
  6. Laboratory Worker

        C. The Laboratory Facility
  1. Design
  2. Maintenance
  3. Usage
  4. Ventilation

  D. Components of the Chemical Hygiene
                  Plan
  1. Basic Rules and Procedures
  2.  Chemical  Procurement, Distribution,
and Storage
  3. Environmental Monitoring
  4. Housekeeping, Maintenance and Inspec-
tions
  5. Medical Program
   6. Personal Protective Apparel and Equip-
 ment
   7. Records
   8. Signs and Labels
   9. Spills and Accidents
   10. Training and Information
   ] 1. Waste Disposal

   E. General Procedures for Working With
                Chemicals
   1. General Rules for all Laboratory Work
 with Chemicals
   2. Allergens and Embryotoxins
   3. Chemicals of Moderate Chronic or High
 Acute Toxicity
   4. Chemicals of High Chronic Toxicity
   5. Animal  Work with Chemicals of High
 Chronic Toxicity

         F. Safety Recommendations

       G. Material Safety Data Sheets

                Foreword

   As guidance for each employer's develop-
 ment of an appropriate laboratory Chemical
 Hygiene Plan, the following non-mandatory
 recommendations are provided. They were
 extracted from "Prudent Practices for Han-
 dling Hazardous Chemicals in Laboratories"
 (referred to below as "Prudent Practices"),
 which was published In 1981 by the Nation-
 al Research  Council and  is available from
 the National Academy Press. 2101 Constitu-
 tion Ave., NW., Washington DC 20418.
   "Prudent Practices" is cited because of its
 wide distribution and acceptance and be-
 cause of its  preparation by members  of the
 laboratory community through the sponsor-
 ship  of the  National  Research Council.
 However, none of  the  recommendations
 given here will modify any requirements of
 the  laboratory  standard.  This Appendix
 merely presents pertinent recommendations
 from "Prudent Practices", organized  into a
 form convenient for quick reference during
 operation of a laboratory facility and during
 development  and application of a Chemical
 Hygiene Plan. Users of this appendix should
 consult "Prudent  Practices"  for a more ex-
 tended  presentation and  justification for
 each recommendation.
  "Prudent  Practices"  deals  with   both
 safety and chemical hazards while the labo-
 ratory standard is concerned primarily with
 chemical hazards. Therefore, only those rec-
 ommendations  directed primarily toward
 control of toxic exposures are cited in this
 appendix, with the term "chemical hygiene"
 being substituted for  the  word  "safetjt".
However,  since  conditions  producing or
 threatening physical injury often pose toxic
risks as  well, page references  concerning
major categories of safety  hazards in the
laboratory are given In section F.
                                              383

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  § 1910.1450
    The   recommendations  from  "Prudent
  Practices"  have  been  paraphrased,  com-
  bined, or otherwise  reorganized, and  head-
  ings have been added. However, their sense
  has not  been changed.

  Corresponding Sections of the Standard and
                this Appendix
    The following table is given  for the con-
  venience of those  who  are  developing a
  Chemical Hygiene Plan which will satisfy
  the requirements of  paragraph (e) of the
  standard. It indicates those sections of this
  appendix which are most pertinent to each
  of the sections of paragraph (e) and related
  paragraphs.
   Paragraph and topic in laboratory standard
 (e)(3)(i)  Standard  operating  procedures lor
   handling toxic chemicals.
 (e)(3)(n)  Criteria to be used  for implementa-
   tion ot measures to reduce exposures.
 (e)(3)(m)  Fume hood performance	
 (e)(3j(iv)  Employee information  and training
   (including emergency procedures).
 (e)(3)(v)  Requirements for prior approval of
   idooratory activities.
 (e)(3)(vi)  Medical  consultation and  medical
   examinations.
 (e)(3)(vn) Chemical hygiene responsibilities	
 (e)(3)(vui)  Special  precautions for work  with
   particularly hazardous substances.
 Relevant
 appendix
  section
C. D. E

0

C4b
010. 09

E2b. E4b

05, E4f

B
E2, E3. E4
  In  this appendix, those recommendations
 directed primarily at administrators and su-
 pervisors are given in sections A-D. Those
 recommendations of primary concern to em-
 ployees who are actually handling laborato-
 ry chemicals are given in section E. (Refer-
 ence  to page  numbers in "Prudent Prac-
 tices" are given in parentheses.)

    A. General Principles for Work with
           Laboratory Chemicals
  In  addition to the more detailed recom-
 mendations  listed below  In sections B-E.
 "Prudent Practices" expresses certain gen-
 eral principles, including the following1
  1. It is prudent to minimize all chemical
 exposures. Because few laboratory chemicals
 are without hazards, general precautions for
 handling all laboratory chemicals should be
 adopted, rather than specific guidelines for
 particular  chemicals (2,  10). Skin contact
 with chemicals should be avoided as a cardi-
 nal rule (198).       ,
  2. Avoid underestimation of risk. Even for
substances of no known significant hazard,
exposure  should  be minimized;  for work
with substances which present special haz-
ards,  special precautions should be  taken
(10. 37, 38). One  should assume that any
        29 CFR Ch. XVII  (7-1-92  Edition)

  mixture  will  be  more  toxic than  its most
  toxic component (30. 103) and that all sut
  stances  of unknown toxicity  are  toxic  (i
  34).
    3. Provide adequate ventilation. The best
  way  to  prevent exposure to  airborne sub-
  stances  is to prevent their escape  into  the
  working  atmosphere by  use of  hoods and
  other ventilation devices (32, 198).
    4. Institute a chemical hygiene program. A
  mandatory chemical hygiene  program  de-
  signed to minimize exposures  is needed; it
  should be a regular,  continuing  effort, not
  merely a standby or short-term activity (6,
  11). Its recommendations should be  followed
  in academic teaching laboratories as well  as
  by full-time laboratory workers (13).
   5.  Observe the PELs, TLVs. The Permissi-
  ble  Exposure  Limits  of   OSHA and the
  Threshold Limit Values  of  the American
  Conference of  Governmental Industrial Hy-
  gienists should not be exceeded (13).

    B. Chemical Hygiene Responsibilities

   Responsibility for chemical hygiene rests
 at all levels (6,  11. 21) including  the;
   1.  Chief executive  officer, who has ulti-
 mate  responsibility  for  chemical  hygiene
 within the institution and must, with other
 administrators, provide  continuing  support
 for institutional chemical hygiene (7, 11).
   2. Supervisor of the department or othefl
 administrative unit,  who  is responsible for
 chemical hygiene in that unit (7).
   3. Chemical hygiene officers), whose ap-
 pointment is essential (7) and who must:
   (a)  Work  with administrators and  other
 employees to develop and implement appro-
 priate  chemical hygiene policies  and prac-
 tices (7);
   (b) Monitor procurement, use, and dispos-
 al of chemicals used in the  lab (8);
   (c)  See  that appropriate  audits  are main-
 tained (8);
   (d) Help project directors develop  precau-
 tions and adequate facilities (10);
  (e)  Know the current  legal requirements
 concerning regulated substances (50); and
  (f) Seek  ways  to improve  the chemical hy-
 giene program (8, 11).
  4. Laboratory supervisor, who has overall
 responsibility for chemical hygiene in the
 laboratory (21) including  responsibility to:
  (a)  Ensure that workers  know and follow
 the chemical hygiene rules, that protective
 equipment is available and in working order,
 and'that appropriate training has  been pro-
 vided (21, 22);
  (b)  Provide regular,  formal chemical hy-
 giene and  housekeeping inspections  includ-
 ing routine inspections of emergency equip-
ment (21. 171);
  (c) Know the  current legal requirements
concerning regulated substances (50. 231);
                                         384

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    (d) Determine the required levels of pro-
   tective apparel  and equipment  (156, 160,
   182); and
    (e) Ensure that facilities and training for
   use of any material being ordeied are ade-
   quate (215).
    5. Project director or director of other spe-
   cific operation, who has primary responsi-
   bility for  chemical  hygiene procedures for
   that operation (7).
    6. Laboratory worker, who is responsible
   for:
    (a) Planning  and conducting each  oper-
   ation  In accordance  with the  Institutional
   chemical hygiene procedures (7, 21, 22, 230);
   and
    (b) Developing good personal chemical hy-
   giene habits (22).

          C. The Laboratory Facility

    1. Design. The laboratory facility should
  have:
    (a) An  appropriate - general ventilation
  system (see C4 below) with air intakes and
  exhausts located so  as  to  avoid  intake of
  contaminated air (194);
    (b) Adequate,  well-ventilated stockrooms/
  storerooms (218, 219);
    (c) Laboratory hoods and sinks (12, 162);
    (d) Other safety equipment including eye-
  wash fountains and  drench showers  (162,
  169); and
   (e) Arrangements for waste disposal (12,
  240).
   2.  Maintenance. Chemical-hygiene-related
  equipment  (hoods, incinerator, etc.) should
  undergo continuing appraisal and be modi-
  fied  if inadequate (11, 12).
   3.  Usage.  The work conducted  (10) and its
 scale (12) must be appropriate to the physi-
 cial facilities available and. especially, to the
 quality of ventilation (13).
   4.  Ventilation—(a)  General  laboratory
 ventilation. This system should: Provide a
 source of air for breathing and for input to
 local ventilation devices (199); it should not
 be relied  on for protection from toxic sub-
 stances released into  the laboratory (198);
 ensure that laboratory air is continually re-
 placed, preventing increase of air concentra-
 tions of toxic substances during the working
 day (194); direct air flow into the laboratory
 from non-laboratory areas and  out to the
 exterior of the building (194).
  (b)  Hoods.  A  laboratory  hood  with 2.5
 linear feet of hood space per person should
 be provided for every  2  workers if they
 spend most of  their time working  with
 chemicals (199);  each hood  should have a
 continuous  monitoring device to allow  con-
 venient confirmation of adequate hood  per-
 formance before use (200. 209). If this is not
 possible, work with substances of unknown
 toxicity should  be  avoided  (13)' or  other
types of local ventilation devices should be
provided (199). See pp. 201-206 for a discus-
  sion of hood design, construction, and eval-
  uation.
    (c) Other local ventilation devices. Venti-
  lated storage cabinets, canopy hoods, snor-
  kels, etc. should be provided as needed (199).
  Each canopy hood and snorkel should have
  a separate exhaust duct (207).
    (d) Special ventilation  areas. Exhaust air
  from glove boxes and Isolation rooms should
  be passed  through scrubbers or other treat-
  ment  before release  into  the regular ex-
  haust -system (208). Cold rooms and warm
  rooms  should have  provisions   for  rapid
  escape and for escape in the event of electri-
  cal failure (209).
    (e) Modifications. Any alteration  of the
  ventilation system should be made only if
  thorough testing indicates that worker pro-
  tection from airborne toxic substances will
  continue to be adequate (12, 193, 204).
    (f) Performance.  Rate:  4-12   room  air
  changes/hour is normally adequate general
  ventilation if local exhaust  systems such as
  hoods  are  used as the primary method of
  control (194).
   (g) Quality. General air flow should not
  be turbulent And  should  be relatively  uni-
  form throughout  the  laboratory,  with no
  high velocity or static areas (194, 195); air-
  flow into and within the hood should not be
  excessively turbulent (200); hood face veloci-
  ty should be adequate (typically 60-100 Ifm)
  (200, 204).
   (h) Evaluation.  Quality and quantity of
 ventilation should be evaluated on installa-
 tion (202),. regularly  monitored   (at  least
 every 3 months) (6, 12, 14,  195), and reevalu-
 ated whenever a change in local ventilation
 devices is made (12, 195, 207). See pp. 195-
 198 for methods of evaluation and for calcu-
 lation of  estimated airborne contaminant
 concentrations.

   D. Components of the Chemical Hygiene
                   Plan

 1. Basic Rules and Procedures (Recommen-
  dations for  these are  given in section E,
  below)

 2. Chemical Procurement, Distribution, and
                 Storage

  (a) Procurement.  Before a substance is re-
ceived,  information  on proper  handling,
storage,  and disposal should be known to
those who  will  be involved  (215,  216).  No
container should  be accepted without an
adequate identifying label (216). Preferably,
all  substances should be received  in a cen-
tral location (216).
  (b) Stockrooms/storerooms.  Toxic  sub-
stances should be segregated in a well-iden-
tified area  with local exhaust  ventilation
(221). Chemicals which are  highly toxic
(227) or  other chemicals whose containers
have been opened should be in unbreakable
                                                385

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   § 1910.1450
                                                    29 CFR Ch. XVII (7-1-92 Edition)
•D,
  secondary containers (219). Stored  chemi-
  cals  should be  examined  periodically (at
  least annually) for replacement, deteriora-
  tion, and container integrity (218-19),
   Stockrooms/storerooms  should not be
  used as preparation or repackaging areas,
  should  be open during  normal working
  hours,  and  should be  controlled by one
  person (219).
   (c)  Distribution.  When  chemicals are
  hand  carried,  the  container   should be
  placed  in  an outside  container  or bucket.
  Freight-only elevators should be used if pos-
  sibk (223).
   (d) Laboratory storage. Amounts permit-
  ted should be as small as practical. Storage
  on  bench tops and in hoods is inadvisable.
  Exposure to heat or direct sunlight should
  be  avoided. Periodic inventories  should be
  conducted, with unneeded items being dis-
  carded or returned to  the storeroom/stock-
  room (225-6. 229).

        3. Environmental Monitoring
   Regular  instrumental  monitoring of air-
  borne concentrations is not usually justified
  or practical in laboratories but may be ap-
  propriate when testing or redesigning  hoods
  or other ventilation devices (12)  or when a
 highly toxic substance  is stored or used reg-
  ularly (e.g., 3 times/week) (13).

     4. Housekeeping. Maintenance, and
                Inspections

   (a) Cleaning. Floors should be cleaned reg-
 ularly (24).
   (b) Inspections. Formal housekeeping and
 chemical hygiene inspections should be held
 at least  quarterly (6,  21) for units  which
 have frequent pesonnel changes  and  semi-
 annually for others; informal inspections
 should be continual (21).
   (c)  Maintenance.  Eye  wash   fountains
 should be inspected at  intervals of not less
 than 3 months (6). Respirators for routine
 use should  be inspected periodically by the
 laboratory supervisor (169). Safety showers
 should  be  tested  routinely  (169). Other
 safety equipment should  be inspected  regu-
 larly, (e.g.,  every  3-6 months) (6, 24,  171).
 Procedures  to prevent  restarting  of out-of-
 service equipment  should  be  established
 (25).
  (d) Passageways. Stairways and hallways
 should  not  be used  as storage areas  (24).
 Access to exits,  emergency equipment, and
 utility controls  should never be blocked
 (24).

            5. Medical Program

  (a)  Compliance with regulations. Regular
medical surveillance should be established
 to the extent required by regulations (12).
  (b)  Routine surveillance.  Anyone whose
work involves regular and frequent handling
of lexicologically significant quantities of a
  chemical should consult a qualified physi-
  cian to determine  on an individual b
  whether a regular schedule of medical SUF
  veillance is desirable (11, 50).
    (c) First aid. Personnel trained in first aid
  should be available during working hours
  and an  emergency  room with medical per-
  sonnel should be nearby (173). See pp. 176-
  178 for description of some emergency first
  aid procedures.

     6. Protective Apparel and Equipment

    These should include for each laboratory:
    (a) Protective apparel compatible with the
  required degree of protection for substances
  being handled (158-161);
    (b) An easily accessible drench-type safety
  shower (162,  169);
    (c) An  eyewash fountain (162);
    (d) A fire extinguisher (162-164);
    (e) Respiratory protection  (164-9),  fire
  alarm and  telephone  for  emergency use
  (162) should be available nearby; and
   (f) Other items designated by the labora-
  tory supervisor (156, 160).

                 7. Records

   (a) Accident records should be written and
 retained  (174).
   (b) Chemical Hygiene Plan records should
 document that the facilities and precautions
 were  compatible  with  current knowledge
 and regulations (7).
   (c) Inventory and usage records for high-
 risk substances should be kept as specified
 in sections E3e below.
   (d) Medical records should be retained  by
 the institution in  accordance with the re-
 quirements of state and federal regulations
 (12).

            8. Signs and Labels

   Prominent signs and labels of the follow-
 ing types should be posted:
   (a)  Emergency  telephone  numbers  of
 emergency personnel/facilities, supervisors,
 and laboratory workers (28);
   (b) Identity labels, showing  contents  of
 containers (including waste receptacles) and
 associated hazards (27, 48);
   (c) Location signs for safety showers, eye-
 wash  stations,  other safety  and first aid
 equipment, exits (27) and areas where food
 and beverage  consumption  and storage are
 permitted (24); and
  (d) Warnings at areas or equipment where
 special pr unusual hazards exist (27).

          9. Spills and Accidents

  (a) A written emergency  plan should be
 established and communicated to all person-
 nel; it should include procedures for ventila-
 tion failure (200), evacuation, medical  care,
reporting, and drills (172).
                                         386

-------
    (b) There should be an alarm system to
   alert people in all parts of  the  facility in-
   cluding isolation areas such as cold rooms
   (172).
    ic> A spill control policy should be devel-
   oped and  should include consideration  of
   prevention, containment, cleanup,  and re-
   porting (175).
    (d) All accidents or near accidents should
   be carefully analyzed with  the results dis-
   tributed to all who might benefit (8,  28).

     10. Information and Training Program
    (a) Aim:  To assure that all individuals at
  risk are adequately informed about  the
  work in the laboratory,  its risks, and what
  to do if an accident occurs (5, 15).
    (b)-Emergency and Personal Protection
  Training: -Every  laboratory  worker  should
  know'the location and proper  use of avail-.
  able protective apparel and equipment (154,
  169).
   Some of the  full-time personnel  of  the
  laboratory  should be trained in the  proper
  use of emergency equipment and procedures
  (6).
   Such training as well as first aid instruc-
  tion should be available to (154) and encour-
  aged for (176) everyone who  might need it.
   (c) Receiving- and  stockroom/storeroom
  personnel should know about hazards, han-
  dling equipment, protective apparel, and rel-
  evant regulations (217).
   (d) Frequency of Training: The training
 and education  program should be a regular,
 continuing  activity—not  simply :an  annual •
 presentation (15).
   (e)  Literature/Consultation:   Literature
 and consulting advice concerning chemical
 hygiene should be readily available to*labo-
 ratory personnel, who should  be encouraged
 to use these information resources (14).

        11. Waste Disposal Program.

   (a) Aim: To assure that minimal harm  to
 people,  other organisms, and the  environ-
 ment will result from the disposal of waste •
 laboratory chemicals (5).
  (b) Content (14, 232, 233, 240): The waste
 disposal program should specify how waste
 is to be collected, segregated,, stored,- and  -
 transported and  include, consideration  of
 what materials can be incinerated. Trans- "•
 port from the institution must be in accord-
 ance with DOT regulations (244).
  (c) Discarding Chemical Stocks: Unlabeled
 containers   of   chemicals • and   solutions
 should undergo prompt ^disposal; if partially
 used, they should not be opened (24, 27).
  Before a worker's employment  in the labo-
 ratory  ends,, chemicals  for  which   that
 person was responsible should be discarded .
or returned to storage (226).
  (d) Frequency of Disposal:  Waste should
be removed  from  laboratories to a central
waste storage area at least once per week
  and  from the central waste storage area at
  regular intervals (14).
    (e) Method of Disposal: Incineration in an
  environmentally acceptable manner is  the
  most practical disposal method for combus-
  tible laboratory waste (14. 238. 241).
    Indiscriminate disposal by pouring  waste
  chemicals down  the drain  (14, 231, 242) or
  adding them  to mixed refuse for  landfill
  burial is unacceptable (14).
    Hoods should not  be used  as a means of
  disposal for volatile chemicals (40, 200).
    Disposal by recycling (233, 243) or chemi-
  cal  decontamination  (40,  230)  should  be
  used when possible.

  E. Basic Rules and Procedures for Working
               with Chemicals

    The. Chemical Hygiene Plan should  re-
  quire  that laboratory, workers  know  and
  follow its rules and procedures. In addition
  to. the procedures of the sub programs men-
  tioned above,  these should include the rules
  listed below.

              1. General Rules

   The following should  be  used for essen-
  tially all laboratory work with chemicals:
   (a)  Accidents  and  spills—Eye  Contact:
  Promptly flush eyes with water for a pro-
 •longed period (15 minutes) and seek medical
  attention (33, 172).
 -'  Ingestion: Encourage the  victim to drink
  large amounts of water (178).
  . Skin Contact: Promptly flush the affected
 •area with water (33. 172. 178) and remove
 any  contaminated  clothing  (172. 178).  If
 symptoms persist after .washing, seek medi-
 cal attention (33).
 >  Clean-up. Promptly clean  up spills, using
 appropriate protective apparel  and  equip-
 ment  and proper disposal (24  33). See pp.
 233-237 for specific-clean.-up recommenda-
 tions.
  (b) Avoidance of  "routine" exposure: De-
 velop und encourage safe habits (23); avoid
 unnecessary exposure to chemicals by any
 route (23);
  Do not smell or taste chemicals (32). Vent
.apparatus which may discharge toxic chemi-
 cals (vacuum pumps, distillation columns,
 etc.Unto local exhaustrdevices (199).
  Inspect gloves (157) and test glove boxes
 (208) before use.
 , Do not allow release of. toxic substances in
 cold -rooms and warm rooms,  since these
 have contained  recirculated  atmospheres
 (209).
  (c)  Choice'Of chemicals: Use only those
 chemicals for which  the .quality of the avaify
 able ventilation system is appropriate (13).
  (d)  Eating,  smoking, • etc.: Avoid .eating.
 drinking, smoking, gum chewing, or applica-
 tion of cosmetics in  areas where laboratory
 chemicals are present (22, 24, 32, 40); wash
                                        387
                                                                                                  f".,
                                                                                                  .'A II: ,

-------
 § 1910.1450
        29 CFR Ch. XVII (7-1-92 Edition)
 hands before conducting these activities (23,
 24).
   Avoid storage, handling or consumption of
 food or beverages in storage areas, refrigera-
 tors.  glassware  or  utensils which are also
 used for laboratory operations (23, 24, 226).
   (e) Equipment and glassware:  Handle and
 store  laboratory  glassware  with care  to
 avoid  damage; do not  use damaged glass-
 ware (25). Use extra care with Dewar flasks
 and other evacuated glass apparatus; shield
 or wrap them to contain chemicals and frag-
 ments should  implosion  occur (25). Use
 equipment only for its designed purpose (23,
 26).
   (f) Exiting:  Wash areas  of exposed skin
 well before leaving the laboratory (23).
   (g)  Horseplay: Avoid  practical  jokes  or
 other behavior which might confuse, startle
 or distract another worker (23).
   (h) Mouth, suction: Do not use  mouth suc-
 tion for  pipeting or starting a  siphon (23,
 32).
   (i) Personal apparel: Cpnfine long hair
 and loose clothing (23,  158). Wear shoes  at
 all times in the laboratory  but do not wear
 sandals, perforated shoes, or sneakers (158).
   (j) Personal housekeeping: Keep  the work
 area clean and uncluttered, with chemicals
 and equipment being properly labeled and
 stored: clean up the work  area  on comple-
 tion of an operation or at  the end of each
 day (24).
   (k) Personal protection: Assure  that ap-
 propriate eye protection (154-156) is worn
 by  all persons,  including  visitors,  where
 chemicals are stored or  handled  (22. 23, 33.
 154).
   Wear appropriate gloves when  the poten-
 tial for contact with toxic  materials exists
 (157);  Inspect  the gloves before each use.
 wash  them  before  removal,  and  replace
 chem periodically (157). (A table of resist-
 ance to chemicals of common glove materi-
 als is given p. 159).
  Use   appropriate  (164-168)  respiratory
 equipment when air contaminant concentra-
 tions are not sufficiently restricted by engi-
 neering controls (164-5), inspecting the res-
 pirator before use (169).
  Use any other  protective  and  emergency
 apparel and equipment  as appropriate (22,
 157-162).
  Avoid use of contact lenses in the labora-
 tory unless necessary;   if they  are  used,
 inform supervisor so special  precautions can
 be taken (155).
  Remove laboratory coats immediately on
significant contamination (161).
  (1) Planning: Seek information and advice
 about hazards (7), plan  appropriate protec-
 tive procedures,  and plan  positioning of
equipment before beginning any  new oper-
ation (22. 23).
  (m) Unattended operations: Leave lights
on. place  an appropriate sign on the door,
and  provide for containment  of  toxic sub-
  stances in the event of failure of a utilji
  service (such as cooling water) to an ur
  tended operation (27, 128).
   (n) Use of hood: Use the  hood for oper-
  ations which might result in release of toxic
  chemical vapors or dust (198-9).
   As a rule of thumb, use a hood or other
  local ventilation device when working with
  any appreciably  volatile  substance with a
  TLV of less than 50 ppm (13).
   Confirm  adequate   hood   performance
  before use; keep  hood closed  at  all times
  except when  adjustments  within  the hood
  are being made (200); keep materials  stored
  in hoods  to  a minimum and do not allow
  them to block vents or air flow (200).
   Leave the  hood ''on"  when  it  is not in
  active use if toxic substances are stored in it
  or if it is uncertain whether adequate  gener-
  al laboratory ventilation will be maintained
  when it is "off" (200).
   (o) Vigilance: Be  alert to unsafe  condi-
  tions and  see  that they are corrected when
 detected (22).
   (p) Waste disposal: Assure that the plan
 for each laboratory operation includes plans
 and training for waste disposal (230).
   Deposit  chemical waste  in appropriately
 labeled  receptacles  and follow  all  other
 waste disposal procedures of the Chemical
 Hygiene Plan (22, 24).
   Do not discharge to the sewer concentr
 ed acids or bases  (231); highly toxic,
 odorous, or lachrymatory substances  (231);
 or any  substances which  might  interfere
 with the biological activity of  waste  water
 treatment plants,  create fire or explosion
 hazards, cause structural  damage or  ob-
 struct flow (242).
   (q) Working  alone: Avoid working alone in
 a building; do not work alone in a laborato-
 ry if the  procedures being conducted  are
 hazardous (28).

       2. Working with Allergens and
               Embryotoxins
  (a) Allergens (examples:  diazomethane,
 isocyanates.  bichromates):  Wear  suitable
 gloves to prevent hand contact with  aller-
 gens  or  substances of  unknown allergenic
 activity (35).
  (b)  Embryotoxins (34-5) (examples: organ-
 omercurials,  lead  compounds, formamide):
 If you  are a woman of  childbearing  age,
 handle  these  substances only  in  a  hood
 whose satisfactory performance has  been
 confirmed, using appropriate  protective ap-
 parel (especially gloves) to prevent skin con-
 tact.
  Review each use  of these materials with.
 the research supervisor and review  contu
 ing uses annually or whenever a proceduf
change is made.
  Store  these  substances, properly  labeled,
in an adequately ventilated area in an un-
breakable secondary container.

-------
MI,
 al-

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  a

 cr
 cs
 3d
 ^d
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in
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    Notify supervisors of all incidents of expo-
  sure or spills; consult a qualified physician
  when appropriate.

     3. Work with Chemicals of Moderate
       Chronic or High Acute Toxicity

    EXAMPLES: diisopropylflurophosphate (41),
  hydrofluoric  acid  (43),   hydrogen cyanide
  (45).
    Supplemental rules to be followed in addi-
  tion to those mentioned above (Procedure B
  of "Prudent Practices", pp. 39-41):
    (a) Aim: To minimize  exposure to these
  toxic substances by any route using all rea-
  sonable precautions (39).
    (b)  Applicability: These precautions are
  appropriate for substances with  moderate
  chronic or high acute toxicity used in signif-
  icant quantities (39).
    (c) Location:  Use  and store these  sub-
  stances only  in areas of restricted  access
  with special warning signs (40, 229).
   Always  use  a  hood  (previously  evaluated
  to confirm  adequate  performance with  a
  face velocity of at least  60 linear feet per
  minute)  (40) or other containment  device
  for procedures which may result in the gen-
  eration of aerosols or vapors containing the
  substance (39); trap released vapors to  pre-
  vent their discharge with  the hood exhaust
  (40).
   (d) Personal protection:  Always avoid skin
 contact  by use of  gloves  and Jong sleeves
 (and other protective  apparel as  appropri-
 ate) (39). Always wash hands and  arms im-
 mediately after working with these materi-
 als (40).
  (e) Records: Maintain  records  of  the
 amounts   of   these  materials  on  hand,
 amounts  used, and  the names of the  work-
 ers involved (40. 229).
  (f) Prevention  of spills and  accidents: Be
 prepared  for accidents and spills (41).
  Assure that at least  2 people are present
 at  all times if  a compound in use  is highly
 toxic or of unknown toxicity (39).
  Store breakable containers of these sub-
 stances in chemically  resistant  trays; also
 work and  mount apparatus above such trays
 or cover work and storage  surfaces with re-
 movable,  absorbent, plastic  backed paper
 (40).
  If a major spill occurs outside the hood,
 evacuate the area; assure that cleanup per-
 sonnel wear suitable protective apparel and
 equipment (41).
  (g) Waste: Thoroughly decontaminate or
 incinerate  contaminated clothing or shoes
 (41). If  possible,  chemically decontaminate
 by chemical conversion (40).
  Store contaminated waste in closed, suit-
ably labeled, impervious containers (for liq-
uids, In glass  or plastic bottles  half-filled
with vermicullte) (40).
    4. Work with Chemicals of High Chronic
                  Toxicity

    (Examples:  dimethylmercury and nickel
  carbonyl (48), benzo-a-pyrene (51),  N-nitro-
  sodiethylamine  (54), other human  carcino-
  gens or substances  with high carcinogenic
  potency in animals (38).)
    Further supplemental rules  to  be  fol-
  lowed,  in addition to all  these  mentioned
  above,  for work with substances  of known
  high chronic toxicity (in quantities  above a
  few milligrams to a few grams, depending on
  the substance) (47). (Procedure A of "Pru-
  dent Practices" pp. 47-50).
    (a) Access: Conduct all transfers and work
 •with these  substances  in  a  "controlled
  area": a restricted access hood, glove box, or
  portion  of  a lab, designated for  use of
  highly toxic substances, for which all people
  with access  are aware  of  the substances
  being used and necessary precautions (48).
   (b) Approvals: Prepare a plan for use and
 disposal of these materials  and obtain the
 approval of the laboratory supervisor (48).
   (c) Non-contamination/Decontamination:
 Protect vacuum  pumps against contamina-
 tion by  scrubbers or HEPA filters and vent
 them into the  hood  (49).  Decontaminate
 vacuum  pumps  or  other  contaminated
 equipment, including glassware, in the hood
 before removing them from  the controlled
 area (49, 50).
   Decontaminate the controlled area before
 normal work is resumed there (50).
   (d) Exiting: On leaving a controlled area,
 remove  any protective apparel (placing it in
 an appropriate, labeled container) and thor-
 oughly  wash  hands, forearms,  face, and
 neck (49).
   (e) Housekeeping:  Use a wet  mop or  a
 vacuum  cleaner   equipped  with  a  HEPA
 filter instead of  dry sweeping if  the toxic
 substance was a dry powder (50).
   (f) Medical surveillance: If using lexicolo-
 gically significant quantities of such  a sub-
 stance on  a regular basis (e.g., 3 times per
 week), consult a qualified physician con-
 cerning-  desirability of regular medical sur-
 veillance (50).
  (g) Records: Keep accurate records  of the
 amounts of  these substances stored (229)
 and used,  the dates of use,  and names of
 users (48).
  (h) Signs and labels: Assure that the con-
 trolled area  is conspicuously marked with
 warning  and restricted access signs  (49) and
 that all  containers of these substances are
 appropriately  labeled  with   identity and
 warning  labels (48).
  (i) Spills: Assure that contingency  plans,
 equipment, and materials to minimize expo-
sures of people and property in case of acci-
 dent are  available (233-4).
  (j)  Storage: Store  containers  of  these
chemicals  only   in  a  ventilated,  limited
                                                389

-------
 § 1910.1450
       29 CFR Ch. XVU (7-1-92 Edition)
 access (48, 227, 229) area in appropriately la-
 beled,  unbreakable,   chemically  resistant.
 secondary containers (48, 229).
   (k) Glove  boxes: For a negative pressure
 glove box, ventilation rate must be at least 2
 volume changes/hour and pressure  at least
 0.5 inches of water (48). For a positive pres-
 sure glove box, thoroughly check for leaks
 before each use (49).  In either case, trap  the
 exit gases or filter them through a HEPA
 filter and then  release them into the hood
 (49).
   (1) Waste:  Use chemical decontamination
 whenever possible; ensure that containers of
 contaminated  waste  (including  washings
 from contaminated flasks) are transferred
 from the  controlled area in a secondary con-
 tainer under the  supervision of authorized
 personnel (49. 50. 233).

   5. Animal Work with Chemicals of High
             Chronic Toxicity

  (a) .Access: For large scale studies, special
 facilities with restricted access are prefera-
 ble (56).
  (b) Administration  'of the toxic substance:
 When possible, administer the substance by
 injection or gavage instead of in the  diet. If
 administration is  in the diet, use a caging
 system  under negative pressure  or  under
 laminar air flow directed toward HEPA  fil-
 ters (56).
  (c) Aerosol suppression: Devise procedures
 which minimize formation and dispersal of
 contaminated aerosols, including those from
 food,  urine, and  feces (e.g..  use HEPA  fil-
 tered  vacuum  equipment  for  cleaning.
 moisten  contaminated  bedding before  re-
 moval  from  the  cage, mix diets  in closed
 containers in a hood) (55. 56).
  (d) Personal protection: When working in
 the animal room,  wear plastic or  rubber
 gloves, fully buttoned laboratory coat  or
 jumpsuit  and, if needed because of  incom-
 plete suppression of aerosols, other apparel
 and equipment  (shoe and head coverings.
 respirator) (56).
  (e) Waste disposal: Dispose of contaminat-
 ed  animal tissues and  excreta  by inciner-
 ation  if the  available incinerator  can con-
 vert the contaminant to non-toxic products
 (238); otherwise, package the waste  appro-
 priately for burial in  an EPA-approved site
 (239).

       F. Safety Recommendations

  The above recommendations from  "Pru-
dent Practices" do not include those which
are directed primarily toward prevention  of
physical injury rather than toxic exposure.
However,  failure  of   precautions  against
injury will often have the secondary effect
of causing toxic  exposures. Therefore, we
list below  page references for  recommenda-
tions concerning some of the major catego-"
 ries of safety hazards which also have impli-
 cations for chemical hygiene:
 1. Corrosive agents: (35-6)
 2. Electrically powered laboratory appara-
   tus: (179-92)
 3. Fires, explosions: (26. 57-74. 162-4,  174-5,
   219-20. 226-7)
 4. Low temperature procedures: (26, 88)
 5. Pressurised and vacuum operations  (in-
   cluding use of compressed gas cylinders):
   (27.75-101)

        G. Material Safety Data Sheets

   Material safety data sheets are presented
 in "Prudent Practices" for the  chemicals
 listed below. (Asterisks denote that compre-
 hensive material safety data sheets are pro-
 vided).
 •Acetyl peroxide (105)
 •Acrolein(106)
 •Acrylonilrile (107)
 Ammonia (anhydrous) (91)
 •Aniline (109)
 •Benzene (110)
 •Benzo[a]pyrene (112)
 •Bis(chloromethyl) ether (113)
 Boron trichloride (91)
 Boron trifluoride (92)
 Bromine (114)
 •Tert-butyl hydroperoxide (148)
 •Carbon disulfide (116)
 Carbon monoxide (92)
 •Carbon tetrachloride (118)
 •Chlorine (119)
 Chlorine trifluoride (94)
 •Chloroform (121)
 Chloromethane (93)
 •Diethylether(122)
 Diisopropyl fluorophosphate (41)
 •Dimethylformamide (123)
 •Dimethyl sulfate (125)
 •Dioxane (126)
 •Ethylene dibromide (128)
 •Fluorine (95)
 •Formaldehyde (130)
 •Hydrazine and salts (132)
 Hydrofluoric acid (43)
 Hydrogen bromide (98)
 Hydrogen chloride (98)
 "Hydrogen cyanide (133)
 •Hydrogen sulfide (135)
 Mercury and compounds (52)
 •Methanol(137)
 •Morpholine (138)
 •Nickel carbonyl (99)
 •Nitrobenzene (139)
 Nitrogen dioxide (100)
 N-nitrofcodiethylamine (54)
 •Peracetic acid (141)
 •Phenol (142)
 •Phosgene(143)
 •Pyridme (144)
 •Sodium azide (145)
 •Sodium cyanide (147)
Sulfur dioxide (101)
                                       390

-------
n)
Occupational Safety ana neaun /Aumm.,
'II         "Trichloroethylene (149)
          •Vinyl chloride (150)

           APPENDIX B TO 5 1910.1450—REFERENCES
                     ( NON-MANDATORY)
           The following references &ve  provided to
          assist the employer in the development of a
          Chemical  Hygiene   Plan.  The  materials
          listed below are  offered  as non-mandatory
          guidance.  References listed  here' do not
          imply specific endorsement of a book, opin-
          ion, technique, policy or  a specific solution
          for a safety or health problem. Other refer-
          ences not  listed here may better meet the
          needs of a specific laboratory, (a) Materials
          for the development  of the Chemical Hy-
          glene Plan:
           1. American Chemical Society,  Safety in
         Academic  Chemistry Laboratories, 4th edi-
         tion.  1985.
           2. Fawcett,  H.H. and W. S. Wood, Safety
         and Accident  Prevention in Chemical Oper-
         ations. 2nd edition, Wiley-Intersclence, New
         York. 1982.
           3.   Flury,   Patricia  A.,  Environmental
         Health and Safety in the Hospital Laborato-
         ry, Charles C. Thomas  Publisher, Spring-
         field IL, 1978.
          4.  Green, Michael E.  and  Turk.  Amos,
         Safety in Working with Chemicals, Macmil-
         lan Publishing Co.. NY, 1978.
          5. Kaufman, James A.. Laboratory Safety
         Guidelines, Dow  Chemical Co.. Box 1713,
         Midland, MI 48640. 1977.
          6.  National   Institutes  of Health.  NIH
         Guidelines  for the Laboratory use of Chemi-
         cal Carcinogens.   NIH Pub. No.  81-2385.
         GPO, Washington, DC 20402. 1981.
          7. National  Research  Council.  Prudent
         Practices for  Disposal of  Chemicals from
         Laboratories,  National   Academy  Press,
         Washington, DC. 1983.
          8. National  Research  Council,  Prudent
        Practices for  Handling Hazardous Chemi-
        cals  in Laboratories. National  Academy
        Press, Washington. DC. 1981.
          9. Renfrew.  Malcolm, Ed., Safety in the
        Chemical Laboratory, Vol. IV. J.  Cfiem. Ed.,
        American  Chemical  Society.  Easlon,  PA.
        1981.
          10. Steere. Norman  V.. Ed., Safety in the
        Chemical Laboratory, J. Chem. Ed. Ameri-
        can Chemical  Society, Easlon.  PA.  18042,
        Vol. I. 1967, Vol. II. 1971, Vol. Ill 1974.
          11. Steere. Norman  V.. Handbook of Labo-
        ratory Safety, the Chemical Rubber Compa-
        ny Cleveland. OH. 1971.
          12. Young. Jay A..  Ed..  Improving Safety
        in the Chemical Laboratory, John Wiley 
-------
 § 1910.1499

 ardous Liquids. Professional Safety pp 15-
 18, April 1980.
  4. National Fire Protection Association.
 Fire  Protection -for  Laboratories  Using
 Chemicals NFPA-45, 1982.
  Safety  Standard  for  Laboratories  in
 Health Related Institutions.  NFPA, 56c.
 1980.
 - Pire. Protection Guide on  Hazardous Ma-
 terials, 7th edition, 1978.
  National Fire Protection Association, Bat-
 terymarch Park, Quincy, MA 02269.
  5. Scientific  Apparatus' Makers  Associa-
 tion  (SAMA),   Standard  for Laboratory
 Fume  Hoods. SAMA LF7-1980, 1101  16th
 Street, NW., Washington. DC 20036.
  (d) Information on Availability of Refer-
 enced Material:
  1. American National Standards  Institute
 (ANSI).  1430 Broadway.  New  York, NY
 10018.
  2. American Society for Testing and Mate-
 rials (ASTM). 1916 Race Street,-Philadel-
 phia. PA 19103.
 (Approved  by the Office of  Management
 and Budget  under control  number  1218-
 0131)
 [55 FR 3327. Jan. 31. 1990; 55 FR 7967. Mar.
6, 1990; 57 FR 29204, July 1, 1992J
       29 CFR Ch. XVII (7-1-92 Edition)

 § 1910.1499  Source of standards.
 Section 1910.1000.
41  CFR  50-204.50,  except1 ._.
 Table 2-2, the source of whicn
 is American National Stand-
 ards Institute, 237 series
 [40 FR 23073, May 28, 1975]

 § 1910.1500  Standards organizations.

  Specific  standards  of the  following
 organizations have been referred to  in
 this subpart.  Copies  of the standards
 may  be obtained from the issuing or-
 ganization.

 American Conference of Governmental
  Industrial Hygienists
 6500 Glenway Avenue. Bldg. D—7
 Cincinnati, Ohio 45211—4438
American National Standards Institute
 1430 Broadway
New York. New York 10018
[40 FR 23073. May 28. 1975. as amended  at
43 FR 57603. Dec. 8. 1978; 57 FR 29204, July
1, 1992]
                                       392

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                                              SECTION:  APPENDIX J, ATTACHMENT 2
                                              VERSION:  FINAL/MAY 1993
                                              PAGE: 1  of 13
Attachment 2 - STORAGE FOR CHEMICAL COMPATIBILITY

Chemicals in the R/V Lake Guardian Laboratories have been color labeled and should be stored
by hazard group according to the following chart:
Group Number       Hazard Classification
                                 Color
                    Acids
                                 Red
II
Flammable
Orange
III
Refrigerated Materials
Green
IV
Oxidizers
Yellow
V
Alkalis
Blue
VI
Extremely Poisonous
Brown
VII
Generally Safe
White
VIII
Special Storage
Black

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                                                SECTION- APPENDIX J, ATTACHMENT 2
                                                VERSION: FINAL/MAY 1993
                                                PAGE: 2 of 13
CHEMICAL STORAGE INVENTORY

All chemicals must be  stored  in  segregated  areas  by compatible chemicals.   Incompatible
chemicals may "eact violently with each other or form highly toxic gases.  These reactions  can
occur  by mixing  incompatible vapors  or  spilling  incompatible  contents  onto  each other.
Therefore, extreme care  must be taken  to  keep  compatibles  segregated  physically  from
incompatibles, e.g., storage of incompatibles on a bottom shelf of a cabinet is not an acceptable
segregating procedure.  Storage areas must be kept in  a cool,  adequately ventilated area.   All
flammable or combustible materials and high heat sources must  be kept  clear of storage areas.
Storage areas  should not be  in a  "heavy traffic"  area  and should be protected from physical
damage.   Storage cabinets must be clearly labeled as to what materials can be  stored  in them.
An inventory  of chemicals  in each storage  area  must  be  kept updated.    New chemicals
purchased must  be  checked  to see what compatible materials they  may be  stored  with by
reviewing MSDS's,  e.g., not all acids can  be stored together.

Chemicals should  not  be stored   in cabinets  under fume  or heat  hoods  where chemicals
procedures take  place.   Chemicals should not be stored  in  direct sunlight.   Chemicals  listed
within a group may  be stored  together.  Groups of chemicals must be physically separated.

Chemical Storage Group I. Most Acids

In general, keep  most acids separate from oxidizers and bases.

Chemical                   Comments

Glacial Acetic Acid           Keep  from hydroxyls, ethyleneglycol,  peroxides,  permanganates,
                            chromic acid, nitric acid, per-chloric acid
1 - Amino-2-Naphthal
-4-Sulfonic Acid

Ammonium Phosphate

Ascorbic  Acid

Disodium Ethylenediamine
Tetracetate

EDTA Tetrasodium Salt

L-( + )-Glutamic  Acid

Glutaraldehyde

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                                               SECTION:  APPENDIX J, ATTACHMENT 2
                                               VERSION:  FINAL/MAY 1993
                                               PAGE: 3 of 13
Chemical

B-Glycerophosphoric Acid

Disodium Salt

Hydrochloric Acid

Lugol's Solution

Oxalic Acid

Phosphoric Acid

Plantinum  Cobalt Color Standard

Sulfuric Acid

Kodak Farmer's Reducer (Thio-
sulfuric Acid Disodium Salt)
Comment
 Keep from sulfuric acid

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                                               SECTION:  APPENDIX J, ATTACHMENT 2
                                               VERSION:  FINAL/MAY 1993
                                               PAGE: 4 of 13
Chemical Storage Group II, Flammables

All flammables must be  stored in a flammable  liquid storage cabinet.  There shall be no storage
of oxidizers, combustibles or  ignitables in this area.

Chemicals                                      Comments

Acetone

Ethyl Alcohol

Hexane

Isopentyl Alcohol

Isoamyl Alcohol

Methyl Alcohol

Nitromethane

Sodium Borohydride

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                                                 SECTION: APPENDIX J, ATTACHMENT 2
                                                 VERSION: FINAL/MAY 1993
                                                 PAGE: 5 of 13
Chemical Storage Group III. Refrigerated Materials

These materials must be stored refrigerated and no oxidizers, acids or other incompatibles shall
be contained in the refrigerated area.

Chemical                                   Comments

I4C,  Radioactive Material

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                                               SECTION: APPENDIX J, ATTACHMENT 2
                                               VERSION: FINAL/MAY 1993
                                               PAGE: 6 of 13
Chemical Storage Group IV. Oxidizers (in general)

These chemicals must be  kept separate  from acids, combustibles, flammables,  ignitables,  and
other incompatibles.
Chemical

Ammonium Molybdate

Ammonium Persulfate


Ascarite

Ferric Nitrate

Iodine

Manganese Dioxide

Potassium Bichromate

Potassium Nitrate

Potassium Persulfate

Potassium Nitrite

Soda Lime

Sodium Bichromate

Sodium Hypochlorite

Stannous Chloride

Sodium Nitrate

Sodium Nitrite
        Comments
Keep from organics, sodium
peroxide, reducing agents.

Material is not an oxidizer.
Keep from acids.

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                                               SECTION: APPENDIX J, ATTACHMENT 2
                                               VERSION: FINAL/MAY 1993
                                               PAGE: 7 of 13
Chemical Storage Group V. Alkalis

These chemicals contain alkali salts which react with some  acids and other  chemicals.  Keep
from oxidizers.

Chemical                                Comments

Aluminum Standard

Barium Standard

Beryllium Standard

Boron Standard

Calcium Standard

Cobalt Standard

Copper Standard

Iron Standard

Lithium Standard

Magnesium Standard

Magnesium Carbonate

Manganese  Standard

Potassium Hydroxide

Potassium Standard

Soda Lime

Sodium Carbonate

Sodium Hydroxide

Sodium Standard

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                                             SECTION:  APPENDIX J, ATTACHMENT 2
                                             VERSION:  FINAL/MAY 1993
                                             PAGE: 8 of 13
Chemical                               Comments


Strontium Standard

Titanium Standard

Vanadium Standard

Zinc Standard

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                                                SECTION:  APPENDIX J, ATTACHMENT 2
                                                VERSION:  FINAL/MAY 1993
                                                PAGE: 9 of 13
Chemical Storage Group VI, Extremely Poisonous Chemicals

In general,  these must be kept from acids and oxidizers.

There are no cyanides used in any procedures.

Chemicals                                 Comments
Carbon Tetrachloride

Chloroform

Dricote

Formaldehyde

Hydrazine Sulfate

Mercuric Oxide Red

Mercuric Thiocyanate

Methylene Chloride

Methylmercuric Bromide

Methylmercury (II) Hydroxide

Phenol

Platinum Chloride
(Platinizing Solution)

Polychlorinated Biphenyl
Standards

Sodium  Nitroprusside
(Sodium Nitroferricyanide)
Keep from alkalis
Keep from acids,  metals,
oxidizers

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                                                SECTION:  APPENDIX J, ATTACHMENT 2
                                                VERSION:  FINAL/MAY  1993
                                                PAGE: 10 of 13
Chemical Storage Group VII,  Chemicals that can be stored relatively safely.

These chemicals are relatively safe to  store.   In general,  keep acids and oxidizers from these
chemicals.
Chemicals

Ammonium Chloride

Ammonium Sulfate

Antimony Potassium Tartrate
(Potassium Antimonyl Tartrate)

Brij-35

Bio-Rex 70

Buffer Solutions

Cadmium

Calcium Sulfate

Cupric Bromide

Cupric Oxide

Cupric Sulfate, 5-Hydrate
(Copper Sulfate)

Disodium Phosphate
4', 6-Diamidino-2-Phenylindole
Dihydrochloride

Ecoscint

Ferrous Sulfate

Magnesium Nitrate  Hexahydrate

Magnesium Sulfate
        Comments

Keep from acids and alkalis

Keep from oxidizers

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                                               SECTION:  APPENDIX J, ATTACHMENT 2
                                               VERSION:  FINAL/MAY  1993
                                               PAGE: 11 of 13
Chemical

Manganese Sulfate
(Manganous Sulfate)

Mercury Indicator

Methenamine
(Hexamethylenetetramine)

Methylthymol Blue

Monopotassium Phosphate

N-1 -Naphthy lethylenediamine
Dihydrochloride

Phenolphthalein

Phenylethylamine

Potassium Chloride
Kodak Bleach (Potassium Bichromate)

Potassium Iodide

Potassium Phosphate

Potassium Sodium Tartrate

Potassium Biiodate

Potassium Biphthalate

Potassium Sulfate

Primary Calibration Standards

RadiacWash

Silicon Atomic Absorption Standard
              Comments
Keep from acids and oxidizers

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                                               SECTION: APPENDIX J, ATTACHMENT 2
                                               VERSION: FINAL/MAY 1993
                                               PAGE: 12 of 13
Chemical

Sodium Bisulfite

Sodium Bicarbonate

Sodium Borate

Sodium Bromide

Sodium Chloride

Sodium Citrate

Sodium Iodide

Sodium Lauryl Sulfate
(Sodium Dodecyl Sulfate)

Sodium Metaphosphate

Sodium Phosphate

Sodium Potassium Tartrate

Sodium Salicylate

Sodium Silicate

Sodium Sulfate

Sodium Sulfite

Sodium Thiosulfate

Starch

Sulfanilamide
Comments

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                                                SECTION:  APPENDIX J, ATTACHMENT 2
                                                VERSION:  FINAL/MAY 1993
                                                PAGE: 13 of 13
Chemical Storage Group VIII, Special Storage

These chemicals present unique  hazards and cannot be  stored with any group  of chemicals in
this laboratory.   These chemicals must be  stored separately from all other chemicals at  this
laboratory. See MSDS's for special precautions.

Chemicals                          Comments

Nitric Acid                         Incompatible with some acids,  is an oxidizer

Sodium Azide

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                                                   SECTION:  APPENDIX J, ATTACHMENT 3
                                                   VERSION:  FINAL/MAY 1997
                                                   PAGE: 1 of 2
Attachment 3 - STANDARD OPERATING PROCEDURES FOR
HAZARDOUS MATERIAL STORAGE AND SPILL PREVENTION
I.    Introduction

     The use of proper handling procedures  for hazardous materials helps to ensure  a safe working
     environment for persons working in a laboratory.  The special hazards present, because the Lake
     Guardian  laboratories  are  on  board  a  ship, make proper  handling  of chemicals  especially
     important.  The purpose of this document is to present standard operating procedures which will
     provide safe storage of hazardous materials in all laboratory areas and will result in the reduction
     of spills.
II.   Ordering and Procurement of Chemicals

     In order to ensure that adequate storage will be provided for hazardous materials needed during a
     survey, the Chemical Hygiene Officer (CHO) must be  informed of the chemical name, amount,
     and approximate arrival  date of all deliveries to the ship.  If the chemical has not been on board
     previously, an MSDS  must be requested  by the person placing the order.  All MSDSs must  be
     given to the CHO to use in planning for safe handling and hazard communication.

     No chemicals will be accepted for storage without adequate labeling.  Labels shall include at the
     minimum:  identification of the contents, description of hazards, precautionary information,  first
     aid instructions, and spill cleanup procedures.

     Compressed gas cylinders shall not  be  kept  on board unless there  is sufficient equipment  to
     adequately secure them  during sailing.   There will  be no  storage at the  warehouse of gas
     cylinders intended for use on the ship.
III.  Bulk Storage of Hazardous Materials

     Storage  of any one  hazardous material  in  quantities greater  than  one  gallon  (4  liters)  is
     considered bulk storage and is not permitted in the laboratories.  The only exception to this rule
     is the extraction laboratory which contains a flammables cabinet.  All other bulk storage will take
     place in the hazardous materials locker (HML).

     The HML consists of two separately diked storage areas,  each containing appropriately labeled
     cabinets. One storage area is designated for  flammable materials.   The other is designated for
     corrosives, poisonous  and generally safe materials. If cabinet  space is insufficient, do not store
     corrosives in the flammabies cabinets or vice versa. Notify the  CHO.

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                                                    SECTION:  APPENDIX J, ATTACHMENT 3
                                                    VERSION:  FINAL/MAY 1997
                                                    PAGE: 2 of 2
IV.  Transfer to the Laboratory

     All  bottled  hazardous materials  leaving  the HML  must be  in  protective  bottle carriers  or
     transferred to a non-breakable container.  Transfer of chemicals to non-breakable containers must
     take place in the  HML hood.  Materials shall be taken from the HML only in quantities sufficient
     for one work shift or one gallon (4 liters), whichever is less.
V.   Storage in the Laboratory

     Hazardous  materials  in the laboratories must be kept  in containment pans  sufficient to hold the
     entire quantity of the  largest  container.   This applies to both cabinet and countertop storage.
     Countertop storage shall  be  minimized whenever possible to reduce the danger of spills  during
     rough seas.

     Materials  in the fume hoods  shall also  be kept in  containment  pans.  Hood storage shall be
     limited to those  chemicals frequently  used during the present shift  and quantities should  not be
     great enough to impede proper function of the hood.

     Hazardous  materials  shall be stored according to chemical compatibility to reduce the danger of
     reaction following a spill.  The CHO will be  inspecting  laboratory storage and will  determine
     when changes  are necessary.

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                                               SECTION: APPENDIX J, ATTACHMENT 4
                                               VERSION: FINAL/MAY 1997
                                               PAGE: 1 of 3
Attachment 4: WEEKLY INSPECTION CHECKSHEET

Date	

A check mark indicates inspection met with standard.  If conditions are unacceptable, indicate date
corrected or make comment below.
1)

2)

3)

4)

5)

6)

7)

8)

9)

10)

11)

12)

13)
        Bio.     Chem.    Wet     Ext.      Anal.    Prod.
        Lab     Lab      Lab     Lab      Lab     Lab

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                                                  SECTION: APPENDIX J, ATTACHMENT 4
                                                  VERSION: FINAL/MAY  1997
                                                  PAGE: 2 of 3
WEEKLY LABORATORY INSPECTIONS
1)      The eyewash is working properly.

2)      Hoods are free of unnecessary clutter.

3)      Transfer containers are properly labeled.

4)      Hoods are being used at or below the permitted sash height

5)      Waste containers are properly labeled and dated.

6)      All chemicals are kept in proper containment.

7)      Chemicals are stored according to compatibility.

8)      Combustibles are stored away from  heaters.

9)      GFCI outlets are functioning correctly.

10)     Access to spill and first aid kits is unobstructed.

11)     All power cords are in good working condition.

12)     Compressed gas cylinders are properly secured.

13)     All exits are unobstructed.

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                                                  SECTION:  APPENDIX J, ATTACHMENT 4
                                                  VERSION:  FINAL/MAY 1997
                                                  PAGE: 3 of 3
WEEKLY GENERAL SAFETY INSPECTIONS
FOR R/V LAKE GUARDIAN
Date
        Emergency showers are unobstructed.

        Emergency lights are functioning.

        No combustibles are stored near room heaters.

        All fire doors are closed.

         All exits are clearly marked and unobstructed.

        Tool room equipment is clean of accumulated debris and shavings.

        Machine guards are in place on tool room equipment.

        The floor  mat is properly placed in front  of the main electrical control panel  in the engine
        room.

        Circuit boxes are in safe condition and doors are closed.

        Compressed gas cylinders are properly secured.

        All GFCIs are in working condition.

        All extension cords are grounded and not in permanent use.

        Electrical cords  are in good condition.

        No combustibles are stored in the exhaust vent room.

        Gasoline Can self-closing devices working properly.

        Panic Bars on walk-in freezers and refrigerators are functional.

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                                              SECTION:  APPENDIX J, ATTACHMENT 5
                                              VERSION:  FINAL/MAY 1997
                                              PAGE: 1 of 7
Attachment 5:  SOP FOR HAZARDOUS AND COMMON WASTES

  I.    INTRODUCTION

 II.    HAZARDOUS CHEMICAL WASTE

       A.    Hazardous  Waste Storage

            1.    Satellite Storage

            2.    Primary Storage

            3.    Interim and Secondary Storage

       B.    Hazardous  Waste Transport

       C.    Disposal Procedures

            1.    Transporters

            2.    Disposal Facilities

III.    NONHAZARDOUS CHEMICAL WASTE

       A.    Laboratory Non-Hazardous Chemical Waste

            1.    Acid Waste

            2.    Basic  Waste

            3.    Neutral Waste

       B.    Ship Non-Hazardous Chemical Waste

IV.    COMMON WASTE

       A.    Hazardous  Common Waste

       B.    Non-Hazardous  Common Waste

       C.    Recyclables

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                                                  SECTION: APPENDIX J, ATTACHMENT 5           |
                                                  VERSION:  FINAL/MAY 1997                       ™
                                                  PAGE: 2 of 7
                                  R/V LAKE GUARDIAN
 STANDARD OPERATING PROCEDURES FOR HAZARDOUS AND COMMON WASTES
 I.    Introduction

      These standard operating procedures were developed to ensure that waste handling operations
      by  Lake Guardian  personnel comply with local, state, and  federal  regulations.   In  addition,
      proper handling of waste enhances the  safety of personnel on board  the ship and minimizes
      hazards to the environment.   Following the correct procedures  will  help  to ensure that the
      Lake Guardian continues to serve the Great Lakes and the communities along its shores in the
      best possible manner.

      In  its  role  as  a  research vessel, the Lake Guardian generates  two  kinds of  waste.   As  a
      functioning research laboratory,  chemical wastes are produced which  can be  of either  a
      hazardous  or nonhazardous nature.   The  ship's additional function as a vessel on which its
      personnel travel, results in the accumulation of the common wastes of daily living and of ship
      operations.  This document serves as a standard for disposal of both these types of waste.
II.    Hazardous Chemical Waste

      Hazardous chemical  wastes are those listed or defined by  the  Resource Conservation and
      Recovery Act in 40  CFR Part 261.  Some wastes are  listed by  name  in the regulation and
      others  are  included  by  definition  as  having   ignitable,  corrosive,  reactive,  or  toxic
      characteristics.   All  disposal  of hazardous chemical wastes  shall be  carried out under  the
      supervision of the Chemical Hygiene Officer (CHO).

      A.    Hazardous Waste Storage

            Hazardous  chemical  wastes are stored  in three areas, the  satellite area, the primary
            storage area, and the interim or secondary storage area.  The following discusses storage
            and labeling methods for each type of area.

            1.   Satellite Storage

                 Hazardous waste generated during preparation and analytical  procedures is initially
                 stored within  the laboratory  at the site of generation.   Acidic, basic,  and organic
                 solvent wastes are  stored in polyethylene containers.  Containers for PCB disposal
                 greater than 50 ppm are to be made of steel.  Containment pans must be used and
                 must have a  capacity large enough to  hold  the contents of the largest container
                 within its confines.

                 All temporary waste containers must have a hand written label  stating the  chemical
                 name(s) of its contents.  Five gallon containers used  for final disposal will have

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                                       SECTION: APPENDIX J, ATTACHMENT 5
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     color coded waste labels. These labels will be distributed by the Chemical  Hygiene
     Officer.   Laboratory personnel  are responsible only for marking the label  with the
     accumulation start date.  The accumulation start date is the  first day in which waste
     was put  into the container.

     In  laboratory  areas  where  five  gallon  final disposal containers  are unsuitable,
     smaller  temporary  containers  may be  used.   These  containers  must be of the
     specified type (polyethylene, steel, etc.)  and  must be hand labeled as to  chemical
     content.  No glass containers may be used.

     Five waste  streams have  been  identified  for possible  generation  by   GLNPO
     laboratories.   To minimize disposal costs, it is important that none of these waste
     streams  are mixed.   To assist in  identification of waste stream containers, a color
     code system will be used.   All containers  regardless of size will be marked with
     appropriate colored  labels.  When transferring waste from a temporary container to
     a five gallon disposal carboy, the color codes must match.   The following is a list
     of the waste streams  and their designated  labels:

         a.   Yellow - Acid waste  from  the dissolved  oxygen carbon (DOC)  system
             containing  sulfuric acid, chromic acid, metals  including  mercury, and
             water;

         b.  Blue - Basic and  neutral waste, containing  sodium  hydroxide,  mercuric
             suifate, phenol, and water;

         d.  Green  - Organic solvent  waste (e.g.,  methylene chloride) containing PCBs
             in concentrations less than 50 ppm  PCBs;

         e.   Red - Waste solvents including hexane, acetone and methanol;
         f.   Radioactive Symbol - Low level radioactive waste containing Carbon 14.

     There is also a methylene chloride  waste containing approximately 1-2% methylene
     chloride with water.  This  waste is non-hazardous (i.e., handled  as a special non-
     regulated waste) and is discussed in section III.

2.   Primary Storage

     The  primary storage  area  for all  hazardous wastes is the Hazardous Materials
     Locker (HML).  This room is also a  primary storage area for hazardous materials
     before use.  The locker is divided into two storage  areas, one containing the storage
     cabinets for  corrosive  and toxic materials and the other  containing the cabinets for
     flammables.    The two  areas  are  separately  diked  and  a  dry  chemical   fire
     suppression system has been installed.

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                                      SECTION:  APPENDIX J, ATTACHMENT 5           A
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                                      PAGE: 4 of 7
     Hazardous wastes leaving satellite storage shall be taken to the HML for continued
     storage.  If the hazardous waste is in containers other than the five gallon carboys,
     the waste  shall be transferred into  an  appropriately labeled  carboy stored in the
     HML. All waste chemical transfers shall take place in the hood in the HML.

     All flammable wastes shall be stored in the fiammables cabinets.   Corrosive wastes
     shall  be  stored in the corrosives cabinets,  taking care  to separate acidic and basic
     materials.  If cabinets are at  their storage capacity, other carboys for neutral  and
     non-flammable wastes may be stored on the floor in containment pans with a  five
     gallon capacity at the discretion of the Chemical Hygiene Officer.  Care must be
     taken to  see that containers are secured from movement during sailing.

3.    Interim or Secondary Storage

     Secondary storage at a  land facility  has been proposed.   Until that  storage area  is
     completed, interim storage will take  place at the warehouse in Bay City.  Transport
     of hazardous  waste  to interim storage may be performed only by EPA personnel
     following the  established transport guidelines.

     Storage  shall  take place at the warehouse  only when transport for disposal cannot
     be scheduled  to coincide with time  in port.  Storage at the  warehouse should not
     extend beyond five  working  days.   All storage  and  transportation of  hazardous
     waste must be coordinated by  the Chemical Hygiene Officer.

B.   Hazardous Waste Transport

     Hazardous waste must  be transported to secondary storage  by persons  employed
     directly  by the EPA in an EPA owned  vehicle.   Contractors and their employees
     may not transport hazardous waste at any time  and vehicles owned by a  contractor
     or his employees are not acceptable for transport.

     Transportation of hazardous waste to  storage must follow the  guidelines printed in
     Procedures for the Transportation of Hazardous Materials bv Great Lakes National
     Program Office via Government Vehicle, as stated in Appendix S  of this manual.

C.   Disposal Procedures

     Disposal of  all  Hazardous  waste  must  be  made through  an  EPA  contracted
     transport, storage, and  disposal (TSD) facility.   The  CHO or his/her designated
     representative shall be present for the pickup of all wastes.

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                                                    SECTION: APPENDIX J, ATTACHMENT 5
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                                                    PAGE: 5 of 7
                  1.   Transporters

                      The CHO or  representative  shall be  responsible  for  the  accuracy of  the
                      information on the waste manifest by assuring that the labeling, amounts, and
                      waste codes are correct  for each waste stream.   Placarding   of the  transport
                      vehicle   may   be   provided   by  the  transporter,  however,  the  CHO   or
                      representative  must assure that it is correct.  Manifests shall  be signed by  the
                      CHO or representative  and one copy should  be  sent to the  appropriate state
                      agency.  The remaining  generator copy should be filed  in the manifest file in
                      the  laboratory office  and retained  for five years.   The  hazardous waste
                      tracking form  must  be completed, which  documents  the  date of pickup,
                      manifest number,  waste stream  identification number,  and  date  the return
                      manifest is received.

                  2.   Disposal Facilities

                      After any TSD has been contracted  and before  each waste pickup, the CHO
                      must assure   that  any   disposal facilities  used   by the  contractor  are   in
                      compliance with EPA off-site policy.

                      The disposal facility  shall  return a signed copy  of the  manifest to  the ship
                      within 35 days after receiving  the waste.   If this copy is not returned within
                      35  days,  the  CHO shall  attempt to  contact the  disposal  facility.   If not
                      received within 45 days, the  CHO  shall  contact the appropriate  agency  as
                      directed  in 40 CFR 262.42,  or contact the appropriate  state agency.  After
                      receipt,  the generator's manifest copy from the disposal  facility shall be filed
                      with the original copy from the transporter in the laboratory office.
III.    Non-hazardous Chemical Waste

       While many of the chemical wastes from the laboratories can be assumed to be hazardous, a
       few are  non-hazardous.   Some hazardous wastes can be pretreated and then disposed of as
       non-hazardous  waste.  In addition, the ship generates several types of non-hazardous waste.
       This  section will identify specific waste streams  which may be  disposed of as non-hazardous
       and provide guidelines for pretreatment where necessary.

       A.    Laboratory Non-Hazardous Chemical Waste

       Laboratory  chemical  wastes that can disposed of as non-hazardous materials are grouped by
       pH as acidic, basic, or neutral.

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                                                   SECTION: APPENDIX J, ATTACHMENT 5
                                                   VERSION: FINAL/MAY 1997
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             1.   Acidic and Basic Waste

                 Acids (pH less than 2) and bases (pH  greater than 12.5)  without other  chemical
                 contamination may be neutralized  and disposed of in the laboratory  sink.   All
                 neutralizations must take place in a fume hood and pH should be  brought as close
                 to 7 as feasible.

             2.   Non-regulated Waste

                 The  aqueous faction  of the  methylene  chloride   extraction   procedure  is  a
                 non-regulated waste.  It must, however, be treated,  using carbon filters, to remove
                 any   detectable  methylene  chloride before it can be disposed  of into  a sewer
                 system. The procedure used in the ship's laboratory generates a large quantity of
                 this waste which is stored in one of the ship's  holding tanks.  Drains leading to the
                 holding tank  are found in the multi-purpose, extraction, and analytical laboratories.
                 At no  time is any other waste,  including concentrated methylene chloride, to  be
                 added to the tank.  The waste shall be treated by a contractor assigned by the EPA.
       B.   Ship Non-Hazardous Chemical Waste

            Waste from systems operations of the ship consists of engine oil,  oily waste (oil mixed
            with water), antifreeze, graywater (sewage),  and untreated surface water.   All  may  be
            disposed of as non-hazardous waste with the following  considerations:

             1.   Disposal of ship waste shall be the responsibility of the  ship's engineer and project
                 manager.   The CHO shall  be  notified  of the  type  and quantity  of  waste before
                 disposal and  give  approval  of  the transport and  disposal method.  All completed
                 manifests must be forwarded to the CHO immediately.

            2.   Oil and oily waste may be  handled by any local contractor provided it is recycled.
                 If tested and found contaminated or otherwise unrecycleable, it must be disposed of
                 following the guidelines in part II.C.

            3.   Antifreeze  may  be disposed of as non-hazardous provided testing does not show
                 any evidence of contamination.  If contaminated, it must be disposed of following
                 the guidelines in part II.C.
IV.    Common Waste

       Common waste  shall be defined  as any  waste  other than chemical whether generated in the
       laboratory or in  other sections of the ship.

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                                            SECTION:  APPENDIX J, ATTACHMENT 5
                                            VERSION:  FINAL/MAY 1997
                                            PAGE: 7 of 7
A.    Hazardous Common Waste

      Hazardous common waste is generated exclusively in the laboratories.

      Gloves,  paper towels, or other disposables that come into contact with hazardous waste
      must be collected  separately, stored  in appropriately  labeled bags, and disposed of as
      hazardous waste.

B.    Non-Hazardous Common Waste

      In order to maintain the respect and goodwill of the public in the towns and cities where
      the ship takes port, the Lake Guardian must ensure proper disposal of its garbage, trash,
      and other non-hazardous common waste.  Personnel on board are expected to follow the
      guidelines in this section at all times.

      1.   At no time is anything to be thrown overboard, either at port or at sea.

      2.   All garbage and trash generated throughout the ship other than in the laboratories
          shall be placed in the trash compactor in the laundry room.  Compacted trash will
          be  stored  in bags  and tied  securely to the main deck  until a proper  place  of
          disposal is reached.

      3.   Final  disposal of trash shall  take place at a Coast Guard dumpster or shall  be
          picked up directly by a paid trash hauler.  No trash may be disposed of at public
          parks or other public dumping places.

      4.   No  trash disposal shall take place without approval by the captain or the CHO who
          will ensure that  arrangements have  been  made  with the  Coast Guard or trash
          hauler.

      5.   No  excess bags will  be left beside  a full  dumpster unless permission is granted
          from the captain or CHO.

      6.   Aerosol cans, paints, or commercial solvents intended for disposal shall be given to
          the  duty engineer or CHO for storage until proper disposal can be arranged by the
          CHO.

C.    Recycling

      A formal plan for recycling is in  place as described in Attachment 5.1.  Recycling and
      conservative use of  disposable materials  is encouraged whenever possible.   Persons
      working on the ship are encouraged to bring a mug for their personal use while on
      board.

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                                        Seaward Services, Inc.                     APPENDIX  j
                                                                                   Attachment  5.1

                    R/V LAKE GUARDIAN STANDARD OPERATING PROCEDURE

Title:  Recycling Program

Background:  In keeping with USEPA mandates, protection of earth resources and good earth citizenship,  it is
desirable to institute and maintain a recycling program aboard the R/V Lake Guardian.  It is  understood  that
while there may not always be adequate resources ashore to accept recycled materials, the intent of this SOP is to
create a program whereby ALL personnel aboard the ship can participate in a recycling program to the maximum
extent possible without causing undue burden on any one person, crewmember, scientist or guest.

Scope;  This SOP shall apply to all material aboard the ship which is disposed of on a regular and continuous
basis with the exception  of waste from the engine room and waste from the laboratories, which are dealt with
under a separate SOP.

Infrastructure provided: An area of the mess deck shall be set aside for placement of waste containers. Separate
containers shall be provided for plastics, clear glass, metal and "wet" garbage.  The latter is defined as table  and
plate scraps and galley food materials in an otherwise moist state. Waste containers will be fitted with colored
nlastic bags of different colors, of the drawstring type.  Another area of the ship, presently the 0-1  level aft  (on
i >p of the container labs) will  be set aside for storage of the plastic bags removed from the mess deck .           M

Procedure:   Each and every person sailing aboard  the R/V Lake Guardian is encouraged to participate in  the
ship's recycling program.  Each person is responsible for properly disposing of the waste from his or  her
stateroom into the proper waste paper container. Upon completion of each meal eaten aboard, each person shall
dispose of his or her own waste into the proper container. Dirty dishes (which have been scraped clean by the
user) shall  then be placed into the cart provided.  At the end of each  meal, the steward will survey the waste
containers.   Any bag which is fuil will be removed and replaced by a clean one.  Bags which are not full shall
have their  drawstrings pulled tight but  not secured.  At  the beginning of the next meal these bags shall be
reopened for further filling. Full bags (color coded as to contents) will be removed by the steward to the outside
storage  area.  Ship's operating crew will not be responsible for segregation and disposal of trash generated by
others beyond the mess deck repository.'

Twenty four hours prior to arrival in each port the ship's Master will contact the Technical Project Coordinator to
advise ETA and duration of stay. The TPC will make an attempt to identify and locate a facility which will come
to the ship to offload segregated waste.  If such a facility does not exist, or is not available during the ship's port
call, only the "wet" garbage will be discharged from the ship, with all other (recyclable) waste remaining aboard.
This procedure will be  maintained for subsequent port calls until such time as the designated recyclable storage
space is full and it is not reasonably expected that another waste removal source will be found. At this point, all
waste then stored on the O-l level will be discharged from the ship into a common shore side container.

Whenever  the ship is in port and garbage is to be discharged from the ship, either to a common container or to a
recycling facility,  ALL members of the ship's  complement shall be called  upon at that time to assist in removal
of the waste ashore. If a "human chain" is established, the time taken to perform this effort will be minimal.      jg|

To achieve  USEPA's goal of recycling reusable materials, the sum total of the ship's complement is encouraged
to participate in this program, not only that each might have an opportunity to demonstrate his or her own good
earth citizenship, but also to minimize the time and effort required to make this program a success.

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                                                   SECTION:  APPENDIX J, ATTACHMENT 6
                                                   VERSION:  FINAL/MAY 1997
                                                   PAGE: 1  of 2
Attachment 6:  RADIATION SAFETY SOP FOR R/V LAKE GUARDIAN LABORATORIES

Limited use of radioactive materials in the R/V Lake Guardian laboratories is covered under the U.S.
Nuclear Regulatory Commission's Material License (Attachment A) issued to the U.S.  EPA Central
Regional Laboratory (CRL).  By amendment to this license, Carbon  14 and Nickel 63  may be used
for laboratory  research and sample analysis on board the ship.  Use of these materials is subject to the
guidelines enumerated  in the CRL Radiation Safety Manual (Attachment  B)  and,  in addition, to  the
specific Lake Guardian guidelines presented here.

Procurement

All radionuclides must be procured by the CRL Radiation  Safety Officer (RSO) as specified in  the
CRL Radiation Safety Manual (hereafter referred  to as the Manual).  Radionuclides transported to  the
ship must be  carried  by an  EPA owned vehicle driven by an EPA employee.   Shipments must be
accompanied by a bill of lading and arrive just prior to use.

Use Aboard the Ship

Use of radionuclides on board the ship shall be overseen by an Interim RSO who shall be named on
the license and shall carry out the responsibilities assigned to the Interim RSO in the Manual.

Training

The Interim RSO and all other employees  involved with use of radioactive materials on the ship shall
be trained according to Radiation Safety Training  guidelines  in the Manual.

Records

All records of training, disposal, equipment, and inventory shall be kept at CRL. Copies of results of
wipe tests for sealed source devices shall be maintained in the
ship's lab office.

Laboratory Use

The Primary Productivity Lab or any other lab designated for use of radioactive materials shall not be
used at any time, even during port time, for other types of lab  work, storage of supplies, or storage of
personal  items.

Storage and Disposal

Storage of radionuclides shall follow storage guidelines in the  Manual.   No  unsealed radionuclides
shall be left on board when the Interim RSO departs at the end of a Survey.  Solid and  liquid wastes
generated on the ship are to be packaged and labeled as radioactive waste and shall be taken to CRL
for disposal according to the Manual.

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                                                   SECTION:  APPENDIX J, ATTACHMENT 6
                                                   VERSION:  FINAL/MAY  1997
                                                   PAGE: 2 of 2
Spill Procedures

Should a spill or other release of radioactive  materials  occur, procedures outlined in the  R/V Lake
Guardian Contingency Plan part IV B 8, stated  in Appendix R of this manual, shall be followed.

Non-GLNPO Groups

Groups  outside  of GLNPO  using  the  ship's laboratory  facilities  and wishing  to  use  radioactive
materials must observe the following guidelines:

1)     All use of radioactive materials must be approved by the CRL Radiation Safety Officer.

2)     The ship's Chemical Hygiene Officer and Interim RSO, if expected on board, shall be notified
       prior to arrival.

3)     Proof of amendment of NRC license to use the material on board the ship must be provided.

4)     A copy  of the  R/V  Lake Guardian Radiation Safety  SOP shall  be provided  to  the group
       supervisor with the expectation that these  guidelines shall  be observed by persons  using
       radionuclides on the ship.

5)     All radioactive materials and wastes, both liquid and solid, must be removed  by the group at
       departure.

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                                          SECTION: APPENDIX  J, ATTACHMENT 7
                                          VERSION: FINAL/MAY 1997
                                          COVER
Attachment 7 -  CRL RADIATION SAFETY MANUAL

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                                               SECTION:  APPENDIX J, ATTACHMENT 7
                                               VERSION:  FINAL/MAY 1997
                                               PAGE: 1  of 19
Attachment 7:       RADIATION   SAFETY  MANUAL   FOR  CENTRAL   REGIONAL
                    LABORATORY
                                 TABLE OF CONTENTS

       Organization of Radiation Safety Program
       Management of the Radiation Safety Program
       Radiation Safety Training
       General Rules for Working With Radioactive Materials
       Radionuclides and Sealed Source Devices
       Procurement
       Receiving Shipments
       Inventory
       Storage and Use
       Labeling and Marking
Safety Plans                                                                12


Protective Measures                                                         12


Spills and Decontamination                                                   15


Disposal                                                                   16


Records                                                                   18

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                                                   SECTION: APPENDIX J, ATTACHMENT 7
                                                   VERSION: FINAL/MAY 1997
                                                   PAGE: 2 of 19
                               RADIATION SAFETY MANUAL
                           CENTRAL REGIONAL LABORATORY
                                          REGION 5

Central Regional Laboratory (CRL) has established, as part of its overall safety program, this manual
to provide guidelines for the safety and well-being of personnel involved in radiation work.  Although
the facility uses and stores very small quantities of radioactive materials, proper training of workers,
laboratory techniques, and  general housekeeping practices are established and maintained in order to
prevent unnecessary radiation exposure and/or contamination.

Organization of the Radiation Safety Program

Each  person working with radioisotopes  is responsible  for adhering to the safety  and  operational
procedures  discussed in this  document.   The  following  sections  review the management of the
program and key personnel for administering the  radiation safety program.
Management of the Radiation Safety Program

Assurance of an adequate radiation safety program at CRL is the ultimate responsibility of the CRL
Director.  However,  the  actual program implementation is delegated to the Radiation Safety Officer
(RSO).   The  CRL  Health  and Safety Committee provides additional oversight and management
assistance in carrying out the program.

Radiation Safety Officer's Duties:

The  RSO is an  individual appointed by the CRL Director.   The RSO  is qualified  by training and
experience in radiation safety to advise and assist the Director and CRL
staff in the safe use of radioactive material and radiation producing devices. The
RSO is  named on  the Nuclear Regulatory Commission (NRC) license and cannot be changed without
prior approval of the NRC. The RSO's duties include the following:

1.       General overseeing all activities involving radioactive material,
        including conducting routine monitoring.

2.       Determining  compliance with rules and regulations and license
        conditions.

3.       Maintaining an inventory of all  radionuclides at CRL and  limiting the
        quantities of radionuclides to the amounts authorized by the licensee.

4.       Supervising and coordinating the radioactive waste disposal program,
        including keeping waste  storage and disposal records.

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5.      Overseeing distribution of personal monitoring devices and reviewing exposure data.

6.      Performing leak tests on sealed sources.

7.      Supervising decontamination in case of contaminating accidents.

8.      Serving as a member of the CRL Health and Safety Committee.

9.      Processing procurement requests for radionuclides.

10.     Maintaining contamination survey records.

        11.     Ensuring that appropriate protective clothing and equipment are
               available to personnel.

        12.     Recommending to the laboratory director the termination of hazardous or potentially
               hazardous radiological operations.
In addition  to the CRL RSO,  an interim  radiation  safety officer is  appointed  aboard each research
vessel during each limnology cruise that is scheduled to use radioactive materials.  The interim RSO
oversees ship activities  involving  the use of radioisotopes and  ensures that storage and handling
practices are in compliance with  the guidelines  established by  this manual.   The  interim RSO's
responsibilities include those listed in items 1,  2, 5, 6, 7, 8, 10, 11, and 12.

Safety Committee:

The  CRL Safety Committee covers radiation  safety  and disposal issues on an as-needed basis during
its monthly meetings.   Committee members present topics for  discussion and resolution.  If action
outside the meeting is required, the committee chairman designates members to investigate and resolve
issues.  Issues that are beyond the scope of the committee are referred to  CRL upper management or
EPA divisions  as  required;  for example,  the Air and  Radiation  Division  is available  for radiation
safety support.

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                                                    SECTION.  APPENDIX J, ATTACHMENT 7
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Laboratory Personnel:

Each person who is designated as a user or who has contact with any radioactive material and/or is an
operator of an instrument containing a sealed source device is responsible for the following:

        1.      Keeping his/her external exposure to radiation as low as reasonably
               achievable.

        2.      Wearing the prescribed monitoring equipment, such as  a dosimeter, in
               radiation areas.

        3.      Wearing protective clothing, including gloves, whenever contamination is possible  and
               not wearing the protective clothing when outside the laboratory area.

        4.      Wearing respiratory protection devices where needed

        5.      Using pipette filling devices and never pipetting radioactive liquids by mouth.

        6.      Performing  radioactive work within the confines of an  exhaust hood unless previous
               examination has indicated the safety of working in the  open.

        7.      Avoid   eating,  drinking  or   smoking  where  radioactive  materials  are  present.
               Refrigerators must not be used jointly for foods and radioactive materials.

        8.      After work  with radionuclides,  checking immediate areas of hoods,  benches, or  any
               work area where radioactive materials  are being used (through use of low level meters
               or wipe tests) for possible contamination and removing contamination  immediately. If
               removal is not possible, the area shall be clearly  identified and the radiation safety
               officer notified.

        9.      Keeping the work area neat and clean.  Work areas  shall be  free of equipment  and
               materials not  required for the  immediate procedure.   Transport and keep radioactive
               materials in double containers to prevent breakage or  spillage and to ensure adequate
               shielding.   Keep work surfaces covered  with absorbent material,  preferably paper
               lined.

        10.     Labeling and  isolating radioactive waste and  equipment used  in the laboratory.
               Equipment used  for radioactive substances should  not be  used  for other work unless
               the equipment is completely decontaminated prior to being used.

        11.     Immediately  reporting accidental  release,  inhalation,  ingestion  or injury  involving
               radioactive  materials  to the supervisor,  the emergency response team, and  the RSO.
               The individual shall cooperate in all attempts to evaluate his/her exposure.

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                                                    SECTION:  APPENDIX J, ATTACHMENT 7
                                                    VERSION:  FINAL/MAY  1997
                                                    PAGE: 5 of 19
        12.     Carrying out decontamination procedures when necessary and taking adequate steps to
               prevent additional spread of contamination.

        13.     When performing new analyses or working with new radioisotopes, working with the
               RSO and lab safety personnel to develop a safety plan for the analytical procedure.

        14.     Notifying the Hazardous  Waste Disposal Officer (HWDO) of any radioactive waste
               slated  for  disposal  and working with the  HWDO to dispose  of the material  in
               accordance with local, State and Federal regulations.
Postings:

CRL posts its NRC license in accordance with NRC regulations on  the safety bulletin board and in
each  work  area  where  radioactive materials are used.   In  addition,  the following  items  are
conspicuously posted in appropriate areas  at CRL:

               The regulations of 10 CFR 19 and 20

       •       This radiation safety manual

       •       Any  notice   of  violation   involving  radiological   working   conditions,   proposed
               imposition of civil  penalty, or order issued pursuant to 10 CFR 20, Subpart B,  and
               any response submitted to such notices.

       •       Form NRC-3 (revision 6/82 or later) "Notice to  Employees"
Radiation Safety Training

All employees involved in the use of radioactive materials at CRL are required to complete an annual
radiation safety training course.  The  course is also required for new employees or employees who are
transferred to work in areas requiring the use of radioactive materials.  Copies of the radiation safety
manual and the NRC license are provided to each  employee participating in the training.  In addition.
on-the-job training is conducted before an employee is allowed  to  independently  perform analyses
involving radioisotopes.  The following topics are presented and discussed during the training course:

        •       Radioactivity and radioactive decay

        •       Characteristics of ionizing radiation

        •       Man-made radiation sources

        •       Acute effects of exposure to radiation

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                                                    SECTION.  APPENDIX J, ATTACHMENT 7
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        •       Health risks associated with occupational radiation exposures

        •       Special considerations in the exposure of women of reproductive age

        •       Occupational exposure limits

        •       Modes of exposure; internal and external

        •       Basic protective measures; time, distance, shielding

        •       Procedures for maintaining exposure as low as is reasonably achievable

        •       Radiation survey instrumentation;  calibration and limitations

        •       Radiation monitoring programs and procedures

               Contamination  control,  including  protective  clothing and equipment and workplace
               design

               Personnel decontamination

        •       Responsibilities of employees and management

               Interaction  with radiation protection staff

        •       Operational procedures associated with specific job assignments.

An annual meeting is also held with  ancillary personnel (security,  housekeeping, and clerical) whose
duties may require  them to work  in the vicinity of radioactive material.  The ancillary personnel are
informed  where radionuclides are  stored and/or used  at CRL and taught  to recognize the radioactive
warning signs posted in  the laboratory.  These personnel are cautioned to use care in labs where the
radioisotopes may be present, for  example, they are told not to touch or remove equipment on bench
tops.  In  addition,  a written handout is provided to  the supervisors of these personnel;  the  handout
has a statement to  be signed  by the supervisor which  indicates  that the supervisor has read  and
understands the information provided  in the handout,  and will provide such information  to  new
employees.

General Rules for Working With Radioactive Materials

The following consists of rules general to  all activities involving the  use of radioisotopes at  CRL.
Employees should be aware of these rules and use them.

1.      Individuals under the age  of 18 are  not permitted to  enter areas where  open radioisotopes are
        used, nor are they allowed to work with radioactive materials or devices.

2.      High standards of cleanliness and good housekeeping should be maintained in all  radioisotope
        use/storage areas.

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                                                   SECTION:  APPENDIX J, ATTACHMENT 7
                                                   VERSION:  FINAL/MAY 1997
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3.      Personal  belongings  should  not  be  brought  into the  laboratory  where  they  may  be
        contaminated.

4.      Eating, drinking, smoking,  and the  application of cosmetics is  forbidden in any area where
        unsealed radioisotopes are used.

5.      Rubber or  plastic  gloves  and laboratory  coats should  be worn whenever working  with
        radioactive  material.   Gloves  should be presumed to be contaminated and  either cleaned  or
        discarded as radioactive waste.

6.      Faucets, notebooks, calculators,  drawer handles, and so on, should  not  be handled while
        wearing gloves.

7.      Whenever possible, work with  all equipment on easily cleaned trays.  All work surfaces must
        be covered with absorbent paper.

8.      Radioactive material should be kept covered, and must be used and stored behind shielding  of
        a type and amount appropriate to the radionuclide(s) present.

9.      Work  involving potential for airborne radioactivity must be performed  in an approved hood.

10.     Before leaving the laboratory, wash  hands and  remove protective equipment, including lab
        coat.
Radionuclides and Sealed Source Devices

Under the current NRC license, CRL is authorized to have the following radionuclides on site:

                                       Chemical and/or
Element and Number                   Physical Form                        Maximum Amount

Any byproduct material with           Any                                    Not to exceed 2.0
Atomic Numbers between                                                    millicuries, total
3-83, inclusive

Carbon-14                           Any                                    Not to exceed 30.0
                                                                            millicuries, total

Nickel-63                            Foils in Perkin-EImer                    Not to exceed 15.0
                                     Model 6006204 detector cells             millicuries per foil

Nickel-63                            Plated sources in                         Not to exceed 15.0
                                     Hewlett-Packard                         millicuries, per
                                     Model 19235 detector cells               source

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                                                    SECTION: APPENDIX J, ATTACHMENT 7
                                                    VERSION: FINAL/MAY 1997
                                                    PAGE: 8 of 19
Appendix  A contains a copy of the most recent  inventory of radionuclides currently held by CRL.
This appendix is updated biannually.

Procurement

In order to ensure that the amounts on hand do not exceed those allowed by the license, the following
procurement process must be followed when ordering radioactive materials or sealed source devices:

1.  The analyst must discuss the request  for  radionuclides with the  Radiation  Safety  Officer  to
    determine the  method of handling, storing, monitoring for, and disposing of the substance.  This
    must be performed before the procurement request form  is completed.

2.  The procurement requisition is approved and logged by the Radiation Safety Officer.

3.  All incoming radionuclides are delivered to the Radiation Safety Officer, who, in turn, notifies the
    analyst.

4.  The Radiation Safety Officer logs the shipment on the inventory and turns it over to the analyst.

Receiving Shipments

Every  package containing radioactive material  is opened and inspected by the Radiation Safety Officer
as soon as possible after receipt.  The following procedures for opening and inspecting packages are
completed  before the radionuclides are transferred to the analyst:

1.  Disposable plastic gloves  and a  lab coat are worn while inspecting shipment containing unsealed
    radioactive material.

2.  Contamination surveys of packages are performed to  determine whether there is leakage.   Before
    opening the package, the RSO monitors the package  with a meter capable of low-level readings
    and performs a wipe test.  If a reading is higher than background, the Regional Health Physicist is
    called for guidance in handling and opening the package. If both readings are less than or equal
    to  background, the package is opened.  After the package is opened, it is  surveyed again with  a
    low-level meter.  If the reading  is higher than background, the Regional Health Physicist is called
    for guidance.

3.  All received shipments are logged in a book which identifies:

       •       The type of radionuclide received
       •       Serial  number of source and activity at the time of calibration (if sealed)
       •       Serial  number of survey meter used and survey resulls
               Wipe test results
        •       Surveyor's initials

Inventory

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                                                     VERSION:  FINAL/MAY  1997
                                                     PAGE: 9 of 19
Physical inventories are conducted for all radioactive materials, including source devices, biannually.
The RSO is responsible for maintaining the  inventory and ensuring that all radioactive materials  are
accounted for.

The RSO also maintains a running inventory, indicating quantities of materials  on hand, materials on
order,  source devices  present or being repaired  off site, and radioactive  materials for  disposal.  The
RSO uses the running  inventory to ensure that NRC license limits are not exceeded.
Storage and Use

Radioactive materials must be stored  so that the measured ionizing radiation level in areas occupied
by  personnel is  less  than 2.0 millirems/hour  (mR/hr) and  as  low  as reasonably achievable.   If
additional storage space or shielding is needed for materials that exceed  the limits, the  analyst should
consult with  the RSO.

All unsealed radioactive materials  must be labeled clearly and marked in such a way  that personnel
passing through the laboratory will note any materials on a bench  top  that present a radiation hazard.
This requirement is especially critical for emergency response personnel entering the laboratory during
an emergency situation.  Radioactive materials should never be left unattended or unsecured.  Analysts
are responsible for securing materials  in a locked cabinet or other  appropriate storage place when not
using the materials. If the analyst leaves the work area  for lunches or  breaks, a sign should be placed
on the door banning access to other personnel.  Locking the door would  also be helpful to ensure that
unauthorized personnel  do not have access to the materials.

All laboratories and storage areas that contain radioisotopes must be clearly  labeled on all entrances.
The yellow and magenta radiation hazard warning label is appropriate for this purpose.
Labeling and Marking

Walkways and  doors  leading  to  open  radionuclide  work  areas are conspicuously and  properly
identified by the use of warning signs stating "Caution, Radiation Area". All equipment and apparatus
used for liquid scintillation is used exclusively for the purpose of counting and must be labeled with
approved warning tape.   All containers of licensed material must bear durable, clearly visible  labels
identifying the radioactive contents, and these must be stored and locked in  an appropriate cabinet.
Safety Plans

Safety plans must be written for all methods or  procedures involving the  use of radioactive materials
that  are not sealed source devices.   Sealed source device  safety procedures are already included in
safety plans for other methods.  Refer to the CRL Safety Manual for guidance in developing a safety
plan.

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                                                    SECTION:  APPENDIX J, ATTACHMENT 7
                                                    VERSION:  FINAL/MAY  1997
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Protective Measures

In addition to the work rules discussed previously, all employees should attempt to keep the dose of
external  radiation as low  as  reasonably achievable (ALARA) through good work practices.  Keeping
the dose from  external  radiation  at ALARA  levels requires  the  wise use  of time,  distance, and
shielding.  In  every  situation,  these three factors must be considered jointly.  While  shielding  is
desirable in reducing exposure, it must not be  overlooked that doing the job in one-half the time is
just as effective as halving the dose rate with shielding.  Working twice as far from a point source is
as effective as doing a job in one-fourth the time.

Time

Plan ahead; be familiar with the procedures involved for the analysis so that handling time is reduced
and, therefore, the potential exposure to the radioisotope
is minimized.

Distance

The  intensity of a radiation field decreases with the distance from the source.  For a point source the
intensity is inversely proportional to the square of the distance, the same as the light from a flashlight.

Shielding

The  intensity of the radiation field is reduced by the proper placement of suitable shielding material.
The  sealed source devices are shielded by the manufacturer and  wipe tests are conducted semiannually
to ensure that leakage is not occurring.  Following are some general guidelines on shielding methods
for radioisotopes:

    1.   Alpha particles are easily shielded by even a piece of paper.

    2.   Beta particles can be a problem.  Using lead may produce Bremsstrahlung (an  x-ray) which in
        turn  must be  shielded.   Therefore,  analysts should use aluminum or plastic to  shield  Beta
        particles.

    3.   Gamma and x-rays are shielded best by  dense materials such as lead.

    4.   Neutrons are best shielded by hydrogenous materials  such as water and plastics.
 Radiation Monitoring Devices

 A number of radiation monitoring devices are available for use.  Radiation dosimeters are worn by
 personnel  who  work with  sealed source and  open radioisotopes.  Meters  and other equipment  are
 available for monitoring contamination of work areas and lab equipment.

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                                                   SECTION:  APPENDIX J, ATTACHMENT 7
                                                   VERSION:  FINAL/MAY 1997
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Dosimeters

The Region V safety office operates a dosimetry program  to monitor external radiation exposures of
personnel who work with radionuclides. The dosimeter must be worn on the person in the vicinity of
the upper  torso at all times when engaged  in work involving radioisotopes.  The dosimeter must be
worn  outside protective clothing in order not  to impair the measurement of beta dose,  Dosimetry
results are treated as medical monitoring records, and therefore,  are subject to privacy restrictions.

In the event that an exposure to radionuclides is suspected,  bioassay screening is available through the
Public Health Service.  If a person suspects  exposure, he/she must report this  immediately to the
supervisor, the RSO, and the Regional Safety Office.

The NRC's present limit of radiation dose that can be received on the job is 5 rem/year.  The EPA,
however, has set an administrative dose limit in order to control external exposure to be less than 0.5
rem/year.  Every effort should be made to ensure that the dose to any employee is ALARA.

Female employees  who are considering pregnancy or are currently pregnant should be especially
cautious when working with or  near radioactive materials.  Pregnant  females should limit exposure to
levels less than 0.5  rem/year and notify their supervisors and the RSO as soon as they know they are
pregnant.

Monitoring Instruments

Survey meters are available for  use  for monitoring  contamination of equipment and work areas.  The
meters are calibrated  by a qualified company, such as the manufacturer, on an annual  basis.  Any
employee requiring the use of a meter should see the RSO.  The following survey meters are available
at or to CRL:

    •   Ludlum Model 14C Geiger Counter with Model 44-9 Probe
    •   Eberline E520 with HP260 Handprobe
    •    Ludlum Micro-R Survey Meter

The following radiation  detection instruments are available for use at  CRL:

        Ludlum Model 140-5 Alpha Scintillation Counter
    •    Beckman Model LS-333 Beta Liquid Scintillation Counter
       Nucor Model 30 Beta and Gamma Counter
       Nuclear Chicago Model  26501 Beta and Gamma Counter

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                                                    SECTION:  APPENDIX J, ATTACHMENT 7
                                                    VERSION:  FINAL/MAY 1997
                                                    PAGE: 12 of 19
Laboratory Monitoring

The radioisotope work areas are monitored periodically.  The radiation labs are monitored after each
work  session  is  completed.   Work areas aboard research vessels  should  be monitored  after each
limnology cruise.  Wipe tests of sealed sources are conducted every six months.

If monitoring reveals that removable  contamination  is present  in the amount of 200 dpm per 100
square centimeters or greater, decontamination procedures will be implemented immediately.

The RSO will direct any changes in work procedures  or decontamination necessary, and the personnel
involved will be responsible for the required action.  If not complied with, action will be taken by the
CRL director.  The  RSO will coordinate  with the Air and Radiation Division for special monitoring
procedures, such as air sampling.

Spills and Decontamination

It  is  unlikely that a radioactive  material spill  will occur  at  CRL.   Most  radioactive material  is
contained in sealed source devices in laboratory  equipment.  In the event of a spill or  leakage from a
sealed source, the hazardous  materials  contingency plan will be  implemented immediately.  This plan
provides for emergencies involving radioactive materials.

Disposal

Most  radioactive wastes  generated at CRL are low level in nature.  The wastes consist primarily of
sample,  biodegradable scintillation fluids, and   lab  wastes  such  as towels.   The  RSO  should be
consulted when disposing of radioactive materials.

The disposal of waste contaminated with low levels of radioactivity is closely  monitored  at CRL. An
analyst wishing to dispose of radioisotopes must inform the RSO of materials to be  disposed.  The
RSO  then meets with  the analyst to determine  the hazards (e.g.,  RCRA regulated waste  as  well as
being low level radioactive) and the appropriate  disposal method given the hazard characteristics.  The
following discusses the disposal procedures used  at CRL.
Liquid Wastes

Carbon-14 liquid  wastes  generated by GLNPO activities may be  disposed via  the  sanitary sewage
system  if the  scintillation  liquid  used  is  biodegradable  (  nol  regulated   under RCRA)  and
water-miscible.  Ecoscint is such a scintillation fluid.  According to  10 CFR Part 20.303, up to one
Curie of Carbon-14 may be disposed  per year in this manner.  In addition, up to  one Curie combined
of other product isotopes (provided none of the prescribed daily limils or concentration limits for each
isotope is  exceeded)  may  also be disposed in this manner  in a year.  Chicago  Metropolitan  Sewer
District regulations incorporate the NRC regulations by reference.

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                                                    SECTION: APPENDIX J, ATTACHMENT 7
                                                    VERSION: FINAL/MAY 1997
                                                    PAGE:  13 of 19
The  disposal of radioisotopes via the sewer system is closely monitored at CRL.  The following steps
are performed during sewer disposal:

    1.  The disposer must have the authorization of the RSO to pour the wastes down the drain.

   2.  A specific sink must be used exclusively  for the disposal process.  A sign  should  be placed
       near  the sink restricting use by  other personnel during the period  in which it has been set
       aside for disposal.

   3.  Sink  disposals are logged in a book maintained by the RSO.  The log includes the following:
       identity  of disposer,  radioisotope,  total   activity  and  volume disposed, date and time of
       disposal, and sink location.

   4.  The container holding the  low level liquid must be triple rinsed and reserved for holding low
       level liquid wastes only.

   5.  The sink must be decontaminated.  This  is  accomplished through triple washing/rinsing  the
       sink  with  a commercial cleaner such as  Det-O-Jet, or Liquinox.  After the  sink has been
       thoroughly cleaned and dried, a  dry  wipe test should be taken to ensure that  the removable
       contamination  is less than  200  dpm  per  100  square  centimeters.    If the  removable
       contamination  is higher than this number, the sink must be washed/rinsed  until  the  level is
       below that specified.

Solid Wastes

CRL holds a permit for  disposal of solid low level waste at the Washington State disposal site.  All
solid wastes which cannot be  decontaminated are shipped to this site;  for example,  gloves and paper
towels cannot be easily decontaminated.  Wastes that can be decontaminated are cleaned then disposed
of in regular trash. Examples of solid wastes that can be decontaminated are  sample vials and liquid
waste containers.  The following procedures are used for decontamination of solid waste:

1.  If sample vials are to be decontaminated, run a test on about ten containers to determine whether
   the decontamination procedure is appropriate for the vials.

2.  Rinse the container three times with water, pouring the wastewater  down a predetermined sink. If
   the rinsing action does not decontaminate the container satisfactorily,  wash/rinse the  container
   three times with a solution of water and a commercial detergent  such as Det-O-Jet.

3.  Wipe  the  container to ensure that removable contamination  is less than  200 dpm per 100 square
   centimeters or for the entire surface  of the container if less than 100 square centimeters.  If the
   removable contamination is  less than this number, the container may be thrown away as regular
   trash.  If the removable  contamination is greater than allowed, repeat the decontamination process.
   If the procedure continues to  be unsuccessful, dispose of the container as low  level  radioactive
   waste.

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                                                   SECTION:  APPENDIX J, ATTACHMENT 7            |
                                                   VERSION:  FINAL/MAY  1997                        ™
                                                   PAGE: 14 of 19
Solid wastes to be disposed at a low level site are stored in a drum in Room  1045.  Once a drum has
been filled, arrangements will be made for shipment off site.
Records

The RSO is responsible for keeping all records required under NRC regulations and as required by the
license.  Records to be maintained, as stated within the regulation, include personnel training records,
waste disposal records, wipe test and equipment service records, and inventory records.

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                                                          SECTION:  APPENDIX K
                                                          VERSION:  FINAL/MAY 1997
                                                          PAGE:  1 of 17
APPENDIX K:  HEAVY EQUIPMENT PRACTICES FOR SEDIMENT SAMPLING

       *      Knuckle/Pivot Crane

       •      Operational Safety Practices During Use of HIAB Crane

              Deck Safety Practices During Use of Overboard Science Equipment, A-Frames, HIAB
              Crane and Hoists
1.0 Scope

    To provide standard operating procedures for use before, during and after operation of the HIAB
    crane.   These procedures shall be  followed  by all  personnel  aboard  the  R/V  Lake Guardian
    involved with the handling, maintenance, and operation of the HIAB crane.
2.0 Reference

    Crane Inspection & Certification  Bureau (CICB)  Training Manual:   Mobile Cranes  & Rigging
    Considerations. Chapter 1-2.
3.0 Responsibility

    There are several  CICB certified  mobile crane operators aboard the R/V Lake Guardian who will
    assume primary responsibility for handling, maintenance, and operation of the HIAB crane.
4.0 Inspection

    The following items  shall be  inspected on a weekly basis.  Any deficiencies such  as those listed
    shall be carefully  examined  and  a determination made as to whether they constitute a safety
    hazard.

    A. Prior to  initial  use, all new and/or altered cranes shall be inspected to insure compliance with
       provisions contained in the Crane Inspection and Certification Manual.

    B. All  control  mechanisms  shall  be  inspected  for  maladjustments interfering  with  proper
       operation.

    C. All  control  mechanisms  shall  be inspected  for  excessive  wear  of  components and  for
       contamination of lubricants or  other foreign matter.

    D. All safety devices shall be inspected for malfunction.

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                                                       SECTION:  APPENDIX K
                                                       VERSION:  FINAL/MAY 1997
                                                       PAGE: 2 of 17

E.  Inspections for deterioration or leakage in air or hydraulic systems.

F.  Crane hooks shall  be inspected for  deformations, cracks, or having more than 15% in excess
    of normal throat opening or more than a 10 degree twist from the  plane of the  unbent hook.

G.  Inspections are  required  for  rope  reeving  for noncompliance  with  crane  manufacturer's
    recommendations.

H.  Electrical  apparatus  shall be  inspected  weekly  for  malfunctioning,  signs  of  excessive
    deterioration,  dirt and moisture accumulation.

    A complete inspection of the crane shall be performed on a yearly basis by qualified personnel
    (e.g., outside  contractor).  This inspection shall  include all of the requirements above  as well
    as the following items.  Any deficiencies  such as those listed shall be carefully examined and a
    determination made as to whether they constitute a safety hazard.

I.   Deformed, cracked or corroded members  in the crane structure and boom.

J.   Loose bolts or rivets.

K.  Cracked or worn sheaves and drums.

L.  Worn,  cracked or  distorted parts such as pins, bearings,  shafts, gears, rollers,  and  locking
    devices.

M.  Excessive wear on  brake and clutch system parts, linings, pawls and ratchets.

N.  Load, boom angle  and other indicator over their full range, for any significant inaccuracies.

O.  Gasoline,  diesel, electric or other power plants  for  improper performance or noncompliance
    with safety requirements.

P.  Crane  hooks.   Magnetic  particle  or other  suitable  crack  detecting  inspection  should  be
    performed at  least  once  each year.

Q.  Travel  steering, braking and locking devices for malfunctions.


R.  Excessively worn or damaged tires.

A crane which has been idle for a period of one month or more, but less than six months,  shall be
given an inspection conforming with requirements of the weekly inspection above, before  placing
in service.

A crane which has been idle for a period of over six months shall be given a complete inspection
conforming with  requirements of the  weekly and yearly inspections, before placing in service.

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                                                           SECTION:  APPENDIX K
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE.  3 of 17
5.0 Testing

    A.  Prior to initial use, all  extensively repaired or altered cranes should be tested by or under the
        direction of an appointed or authorized person.

    B.  Written  reports shall  be furnished by such persons showing test procedures and confirming the
        adequacy of repairs or alterations.

    C.  Test loads shall not exceed 110% of the rated load at any selected working radius.

    D.  Re-rating test report shall be readily available.

    E.  No cranes shall be re-rated in excess of the original  load ratings unless such rating changes are
        approved by the crane manufacturer or final assembler.


6.0 Maintenance

    A.  A preventative maintenance program  based on the crane manufacturer's recommendations shall
        be established. Dated and detailed  records shall be readily available.

    B.  It  is  recommended  that replacement parts  be  obtained  from  the  original    equipment
        manufacturer.

    C.  Before adjustments and repairs are started on a crane, the  following precautions shall be taken
        as applicable.

        1.  Crane  placed where it will cause the least interference  with other  equipment or operations
           in the  area.

        2.  All controls in the "OFF" position (Activate the  Lockout/Tagout Procedure)

        3.  Starting means rendered inoperative.

        4.  Warning or "out  of order" signs placed on the crane.

        5.  Power plant stopped or disconnected at take-off.

        6.  Boom lowered to the ground if possible or otherwise secured against dropping.

        7.  Lower load block lowered to ground or otherwise secured against  dropping.

    D.  After  adjustments and repairs have been made, the crane  shall not be operated until all guards
        have been reinstalled, safety devices reactivated and  maintenance equipment removed.

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                                                           SECTION:  APPENDIX K
                                                           VERSION:  FINAL/MAY  1997
                                                           PAGE:  4 of 17
7.0 Adjustments and Repairs
    A. Any unsafe conditions disclosed by the inspection shall be corrected before operation  of the
       crane is resumed.  Adjustments and repairs shall be done only by designated personnel.

    B. Adjustments shall be maintained to assure correct functioning of components. (All functional
       operating mechanisms, safety devices, control systems and power plants).

    C. Repairs or replacement shall be provided promptly as needed for safe operation.

    D. All replacement parts or repairs  shall have at least the origina.1 safety factor.

    E. All moving  parts of the crane  for  which lubrication is  specified,  including  rope  and  chain,
       shall  be regularly  lubricated.   Lubricating  systems  shall  be checked  for proper delivery  of
       lubricant.  Particular  care should be taken  to follow manufacturer's recommendations as to
       points and frequency  of lubrication, maintenance of lubricant levels and types of lubricant to
       be used.

    F. Machinery shall be stationary while lubricants are  being  applied and protection provided as
       called for in the above section "Maintenance", unless equipped for automatic  lubrication.


8.0 Rope Inspection

    A. All running ropes in continuous service should be visually  inspected once every working day.
       A thorough  inspection of all rope  in  use shall be  made  at least  once a month  and  a full
       written,  dated  and  signed report of rope conditions must be kept on file in the safety  office.
       All inspections shall be performed  by an appointed, authorized person.  Any deteriorations,
       resulting  in appreciable loss of original strength, such as described  below,  shall be carefully
       noted  and determinations made  as to whether further use of Ihe rope would constitute a safety
       hazard.

        1.  Reduction  of rope diameter  below nominal diameter due to loss of core support, internal or
           external corrosion or wear of outside wires.

       2.  A number  of broken outside wires  and the degree of distribution of concentration of such
           broken wires.

       3.  Worn  outside wires.

       4.  Corroded or broken wires at end connections.

        5.  Corroded,  cracked, bent, worn or improperly applied end connections.

        6. Severe kinking, crushing, cutting or outstanding.

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                                                           SECTION:  APPENDIX K
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE:  5 of 17

    B.  Heavy wear and/or broken wires may occur in sections in  contact with equalizer sheaves or
        other sheaves where rope  travel is limited, or with saddles.  Particular care  shall be taken to
        inspect ropes at these locations.

    C.  All rope which  has been idle for a period of a month or more due to shut down or storage of a
        crane  on which it  is installed shall  be given a thorough  inspection bjfore it is  placed in
        service.  This inspection shall be for all types of deterioration and shall be  performed by an
        appointed, authorized person whose approval shall be required for further use of the rope.  A
        written and dated report of the rope condition shall be filed.

    D.  Particular care shall be taken in the inspection of non-rotating rope.
9.0 Rope Replacement

    A. No precise rules can  be given for  determination of the exact time for  replacement of rope,
       since many variable factors are involved.  Safety in this respect depends largely upon the use
       of good judgement by an appointed, authorized person in evaluating remaining strength in a
       used  rope  after allowance for  deterioration disclosed by inspection.  Safety of rope operation
       depends upon this remaining strength.

    B. Conditions such as the following should be sufficient  reason  for questioning rope safety and
       for consideration of replacement.

       1.  In running ropes, six randomly distributed broken wires in  one lay or  three broken wires in
           one strand in one lay.

       2.  Wear of one-third the original diameter of outside individual wires.

       3.  Kinking,  crushing, bird  caging or any other damage resulting  in distortion of the rope
           structure.

       4.  Evidence of any heat damage from  any cause.

       5.  Reductions from nominal diameter of more than:
           3/64" for diameters to and  including 3/4"
           1/16" for diameters 7/8 to  1 1/8" inclusive
           3/32" for diameters 1 1/4"  to 1 1/2" inclusive

       6.  In standing  ropes, more  than  two broken  wires  in one lay  in sections   beyond and
           connections or  more than one broken wire at an end connection.

    C. In order to establish data as a basis of judging the proper time for replacement, a continuing
       inspection  record shall be maintained.   This record shall cover points on deterioration listed in
       "Inspection" of this section.

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                                                           SECTION:  APPENDIX K
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE: 6 of 17

10.0 Rope Maintenance

    A.  Rope shall be stored to prevent damage or deterioration.

    B.  Unreeling or uncoiling of rope  shall be done as recommended  by the rope manufacturer and
       with extreme care to avoid kinking or inducing a twist.

    C.  Before cutting a rope,  seizing shall be placed on each side of the place where the rope is to be
       cut to prevent unlaying of the strands. On preformed rope,  one  seizing on each side of the cut
       is required.  On non-preformed  ropes of 7/8" diameter or smaller, two seizing on each side of
       the cut are required, and  for non-preformed rope of one inch diameter or larger, three seizing
       on each side of the cut are required.
    D.  During  installation, care  shall be observed to  avoid dragging of the rope  in dirt or around
       objects which will scrape, nick, crush or induce sharp bends in it.

    E.  Rope should  be  maintained  in a  well  lubricated  condition.   It is important that lubricant
       applied as part of a maintenance program shall be compatible with the  original lubricant  and to
       this end the rope manufacturer should be consulted. Those sections of rope which are located
       over sheaves or otherwise hidden during inspection and maintenance procedures require special
       attention when lubricating  rope.  The object of rope lubrication is to reduce internal friction
       and to prevent corrosion.  Periodic field lubrication is particularly important for  non-rotating
       rope.

    F.  When  an operating rope shows  greater wear  at  well  defined localized  areas than  on the
       remainder  of the rope, rope  life  can be extended  in cases where a reduced rope  length  is
       adequate by cutting off a section  at one end, and thus  shifting  the  wear to different  areas of
       the rope.

11.0 Operation

    A.  Cranes shall only be operated by designated operators, learners under the direct supervision of
       a designated operator,  maintenance and test personnel when it is necessary in the performance
       of their duties, and inspectors.

    B.  No one, other than personnel specified in the above paragraph shall  enter a crane  control
       station, with the exceptions of persons  such as  oilers and supervisors, whose duties require
       them  to do  so, and then only  in the performance  of their duties and with the knowledge of the
       operator or  other person.

    C.  Operators shall be required to pass a practical  operating  examination.  Examinations shall be
       limited to the specific  type equipment which he will operate.

    D.  Operators shall meet the following physical qualifications:

           1.         Have vision of at  least 20/30 Snellen in one eye, and  20/50 in the other, with
                      or without corrective lenses.

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                                                        SECTION:  APPENDIX K
                                                        VERSION:  FINAL/MAY 1997
                                                        PAGE: 7 of 17

        2.          Be able to distinguish red, green,  and yellow regardless of position of colors, if
                   color differentiation is required for operation.

        3.          Hearing, with  or  without hearing aid,  must  be adequate  for  the   specific
                   operation.

        4.          A  history of epilepsy or of a disabling heart  condition shall be   sufficient
                   reason  for his/her disqualification.

E.  The  operator shall  not engage in any practice which will  divert his attention while actually
    engaged in operating the crane.

F.  When he is physically or mentally unfit, an operator shall not engage in the operation of this
    equipment.

G.  The  operator shall  respond  to  signals only from the appointed signal man,  but  shall  obey a
    stop signal at any time, no matter who gives it.

H.  If  a warning  signal  is  furnished,   it shall  be  sounded  each  time before  traveling,  and
    intermittently during travel,  particularly when approaching workmen.

I.   Before leaving a crane  unattended, the operator shall:

    1.   Land any attached  load,  bucket, lifting magnet or other device.

    2.   Disengage clutch.

    3.   Set travel, swing, boom  brakes and other locking devices.

    4.   Put controls in the  "OFF" position.

    5.   Stop the engine.

    6.   Secure crane against accidental travel.

    7.   Crane booms shall be  lowered to  ground level  or  otherwise fastened   securely  against
        displacement by wind loads or other outside forces.

J.   If there is a warning sign on the switch or engine starting controls, the operator shall not close
    the  switch or start the engine until the warning sign has been removed by the person  placing it
    there.

K.  Before closing the switch or starting  the engine,  the operator shall see that all controls are in
    the "OFF"  position  and all personnel are in the clear.

L.  If power fails during operation, the operator shall set all brakes and locking devices, move all
    clutch or  other power  controls to the "OFF" position  and, if practical, the suspended load
    should be  landed under brake control.

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                                                           SECTION: APPENDIX K
                                                           VERSION: FINAL/MAY 1997
                                                           PAGE:  8 of 17

    M. The operator shall familiarize himself with the equipment and  its proper care.   If adjustments
       or repairs are necessary, or any defects are known, he shall report the same  promptly to the
       appointed person and shall also notify the  next operator of the defects  upon changing shifts.

    "N. All controls shall be tested by the operator at the start of a new shift.  If any  controls do not
       operate properly, they shall be adjusted or repaired before operations are begun.

    O. Booms which are being assembled or disassembled on the ground with or without support of
       the boom harness should  be securely blocked to prevent dropping  of the boom and boom
       sections.
12.0 Handling and Moving the Load

    A,  No crane shall be loaded beyond the rated load, except for test purposes.

    B.  When loads which are  limited  by  structural competence rather than by stability  are to be
       handled, the person responsible for the job shall ascertain that the weight of the load has been
       determined within plus or minus 10% before  it is lifted.

    C.  The hoist rope shall not be wrapped around the load.

    D.  The load shall be attached to the hook by means  of slings or other approved  devices.

    E.  The individual directing the lift shall see that the crane is level and where  necessary blocked
       properly and that the load is well secured and properly balanced in the sling or lifting  device
       before it is lifted more than a few inches.

    F.  Before starting the hoist, note the following conditions:

       1.  Hoist rope shall not be kinked.

       2.  Multiple part lines shall not be twisted around each other.

       3.  The hook shall be brought over the  load in such a manner as to prevent swinging.

       4.  If there is a slack rope condition, it should  be determined that the rope is  properly seated
           on the drum and in the sheaves.

    G. During hoisting, care shall be taken that there is no sudden acceleration  or  deceleration of the
       moving load and that the load does not  contact any obstructions.

    H. Side loading of booms  shall be limited  to freely  suspended loads. Cranes shall not be used for
       dragging loads sideways.

    I.  The operator shall not hoist, lower, swing or  travel while anyone is on the load or  hook.

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                                                        SECTION:  APPENDIX K.
                                                        VERSION:  FINAL/MAY 1997
                                                        PAGE:  9 of 17
J.   The operator should avoid carrying loads over people.
K.  The operator shall test the brakes each time a load approaching the rated load is handled by
    raising it a few inches and applying the brakes.

L.  Outriggers shall be used when the load to be handled at that particular n.dius exceeds the rated
    load without outriggers as given by the manufacturer for that crane.     Where   floats   are
    used,  they  shall be  securely  attached  to  the outriggers.   Wood  blocks  used to support
    outriggers shall:

    1.  Be strong enough to prevent crushing.

    2.  Be free from defects.

    3.  Be of sufficient width and length to prevent shifting or toppling under load.

M.  Neither the load nor the  booms shall be lowered  below  the point where less than two  full
    wraps of rope remain in their respective drums.

N.  When two or more cranes are used to lift one load, one designated person shall be responsible
    for the operation.  He/she shall analyze  the operation and  instruct all personnel involved  in the
    proper positioning, rigging of the load and the movement to be made.

O.  In transit, the following additional precautions shall  be exercised:

    1.  The boom shall be carried in  line with the direction of motion.

    2.  The superstructure shall be secured against rotation, except when  negotiating turns  when
       there is an operator in the cab or the boom  is supported on a dolly.

    3.  The empty  hook  shall  be  lashed or otherwise restrained so that it cannot swing  freely.

P.  Before traveling a crane with a load, a  designated person  shall be responsible for  determining
    and controlling  safety.  Decisions  such  as  position of load, boom  location,  ground support,
    travel  route and speed of movement shall be in  accordance with his/her determinations.

Q.  A crane with or without load shall not  be traveled  with the boom so high that it  may bounce
    back over the cab.

R.  When rotating  the crane,  sudden starts  and  stops shall be  avoided.  Rotational speed shall be
    such that the load does not swing out beyond the radii at which it can  be  controlled.  A tag or
    restrain line shall be  used  when rotation of the load  is hazardous.

S.  When a crane is to be operated at a fixed radius, the boom-hoist pawl or other positive locking
    device shall be  engaged.

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                                                           SECTION:  APPENDIX K
                                                           VERSION:  FINAL/MAY  1997
                                                           PAGE:  10 of 17
    T.  Ropes shall not be handled on a winch head without the knowledge of the operator.

    U.  While a winch head is being used, the operator shall be within convenient reach of the power
       unit control level.
13.0 Holding A Load

    A.  The operator shall not leave his position at the controls while the load is suspended.

    B.  No person  should be permitted to stand or pass under a load on the hook.

    C.  If the load must remain suspended  for any considerable lenglh of time, the operator shall hold
       the drum from rotating in the  lowering direction by activating the positive controllable means
       at the operators station.

14.0 Signals

    A.  Standard signals  to the operator shall  be in accordance with  the  standards prescribed in  the
       next paragraph,  unless voice  communication  equipment  (telephone,  radio or equivalent) is
       utilized. Signals shall be discernible or audible at all times.  No response shall be made unless
       signals  are clearly understood.

    B.  Hand signals shall be in accordance with  attached diagram.

    C.  For operations not covered by the hand signals, or for special conditions that occur from time
       to time, additions to or modification of the standard signals shall be required.  In such cases,
       these special  signals  shall be  agreed  on  in  advance by the operator and the  signalman and
       should  not be in conflict with standard signals.

    D.  If it is desired to give  instructions to the operator, other than provided by the established signal
       system, the crane motions shall be stopped.

15.0 Miscellaneous

    A.  Cranes  shall not be operated without the  full amount of any ballast or  counterweight in place
       as specified by the maker, but truck cranes that have dropped the ballast or counterweight may
       be operated temporarily  with special  care and  only for  light  loads  without  full ballast  of
       counterweight in place.   The ballast or counterweight in  place specified by the manufacturer
       shall not be exceeded.

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                                                        SECTION:  APPENDIX K
                                                        VERSION:  FINAL/MAY  1997
                                                        PAGE.  II of 17

B.  Except where the electrical  distribution and transmission  lines have  been de-energized and
    visibly grounded at point of work or where insulating barriers not a part of or an attachment to
    the crane have been erected to prevent physical contact with the lines, cranes shall be operated
    proximate to, under, over, by or near power lines only in accordance with the following:

    1.  For lines rated 50kV or below,  minimum clearance between the  lines and any part of the
       crane or load shall be 10 feet.

    2.  For lines rated over 50 kV  minimum, clearance between  the lines and any part  of the
       crane or  load shall be  10 feet plus 0.4  inches for each  IkV over 50kV, or use twice the
       length of the line  insulator but never less than 10  feet.

    3.  In transit with no  load and boom lowered, the clearance shall be a minimum of four feet.

    4.  It is recommended that a person be designated  to observe the clearance and give  timely
       warning for all operations where  it is  difficult for the operator to maintain the desired
       clearance by visual means.

C.  Cage-type  boom  guards,  insulating lines,  or proximately  warning devices may be used on
    cranes, but the use of such devices shall not operate to alter the requirements of paragraph (A)
    above, even if such devices are required by law or regulation.

D.  Before the  commencement of operations near electrical lines, the person responsible for the job
    shall notify the owners of the lines  or their authorized representative providing them with all
    pertinent information and requesting their cooperation.

E.  Any  overhead wire shall be considered to  be an energized line  unless and until the person
    owning such  line or the electrical utility authorities indicate that it is not an energized line.

F.  Exceptions to this procedure, if approved by the  owner of the  electrical lines, may be granted
    by the administrative  or regulatory authority if the alternate procedure provides  sufficient
    protection and is set forth in writing.

G.  Firm footing, uniformly level within one percent  should be  provided.   Where such a  footing is
    not  otherwise supplied, it  should  be  provided by  substantial timbers, cribbing,  or  other
    structural members sufficient to distribute the  load so as not to exceed safe bearing capacity of
    the underlying material.

H.  A carbon dioxide, dry chemical or  equivalent fire extinguisher shall be kept in  the vicinity of
    the crane.   Operating  and maintenance personnel  shall be  familiar with the use and care of the
    fire extinguishers provided.

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                                                        SECTION- APPENDIX K
                                                        VERSION: FINAL/MAY 1997
                                                        PAGE: 12 of 17
Hand signals for use in directing winch or crane operators:
   RETRACT BOOM
                 BOOM DOWN
   vr^1
     n
                  BOOM-UP
                  HOOK DOWN
  IvaneSalm Associates
 REMEMBER!
 EVERYBODY
   MUST
UNDERSTAND
  BEFORE
 ANYTHING
   MOVES
                              EXTEND BOOM
               BOOM-DOWN
                HOOK-UP
        --J3
   r
  .BRIDGE TRAVEL
 OVERHEAD
  CRANES
            TROLLEY TRAVEL  /

                                                                  SAFETY SAVES LIVES
                                                                  CRANE SAFETY ASSOClATeS
                                                                        OF AMERICA ?"j.'
                                                                     -800-356-2212
Hand  Signals: Where  verbal communications are impossible or are likely to be interfered  with  by
other noises, the crew should utilize a standard set of hand signals. Where the winch operator doesn't
have a clear view of the load, one competent crewman should give signals, not the whole crew.

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                                                          SECTION:  APPENDIX K.
                                                          VERSION-  FINAL/MAY 1997
                                                          PAGE:  13 of 17
       DECK SAFETY PRACTICES DURING USE OF OVERBOARD SCIENCE
       EQUIPMENT, A-FRAMES, HIAB CRANE AND HOISTS
1.0 Scope

    To provide standard  operating procedures  and general deck safety practices during the use of
    overboard  science equipment, A-Frames,  HIAB Crane, Hoists or any other systems that  lift,
    lower, secure or otherwise handle objects on board the R/V Lake Guardian.
2.0 References

    Environmental Protection  Agency:   GLNPO Chemical  Hygiene  Plan and  Safety Manual,
    Page 34.

    Environmental Protection Agency, Region 5:  Health and Safety Manual, Section 18-1.

    North Pacific Fishing Vessel Owner's Association: Vessel Safety Manual, Chapter 4, "Working
    Conditions".

    Seaward Services, Inc.:  Occupational Safety & Health Program for Employees Working Aboard
    the R/V Roger R. Simons and R/V Lake Guardian, Section 2-1.
3.0 Responsibility

    The ship's  Master is  responsible for  implementation  and accomplishment  of the  procedures
    contained herein.
4.0 Frequency of Inspection

    Prior to the  use  of any  piece of lifting gear,  crane, A-Frame, davit or other handling device,
    either the ship's  Science Officer, Marine  Science Technician, the mate  on  watch or appointed
    designee, shall visually inspect the equipment involved with the operation and attest to its ability
    to perform the intended operation. This inspection shall include slings, hoists, blocks, ropes and
    associated hardware.  On an annual  basis,  preferably several weeks prior to commencement of a
    new  operating season,  all of the aforementioned gear will  be  inspected  by a qualified and
    independent third party for material  condition  and safe working load.   Straps, chains, shackles,
    turnbuckles, etc., shall be condemned and  removed from the vessel if found  to be unserviceable.
    Cranes, winches, A-Frames and davits  found to be unserviceable shall  be "tagged out of order"
    and repaired  prior to further use.

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                                                           SECTION:  APPENDIX K
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE:  14  of 17
5.0 Deck Skills and Safety Procedures
    A. Work vests shall be worn at all times during sampling and testing conducted on the weather
       decks.

    B. Hard hats must be worn by crane operators and any persons assisting with the load.

    C. All individuals involved in sampling procedures on deck shall wear appropriate safety gear.
       (Life vests, work suits,  hard  hats, eye,  face, hand and foot  protection, safety lines, etc.).
       Employees will  be provided with personal protective equipment  and trained in the use and
       care of this equipment.   In compliance with OSHA 1910  standards, vessel employees are
       required to  adhere  to any and all policies regarding personal protective equipment for their
       own well being.

    D. No one is permitted on the weather decks during sea operations unless directly involved with
       sampling, testing, checking of equipment or other work.

    E. During  weather  conditions causing freezing of water on  deck surfaces, hoses shall be used to
       thaw surfaces with warm water  as needed to prevent a slipping hazard for those working
       outside.  Special precautions are necessary in wet, slippery conditions.

    F. Never stand in a bight (a loop of line, rope or chain) or put any part of your body into a
       bight without first making sure that the gear cannot be worked.

    G. Never stand in the  direct line  of pull when a rope  or wire is under tension, in case it breaks
       and snaps back.   Generally,  rope  or wire snaps back with a corkscrew motion, so the danger
       zone extends to  either side of the direct line of pull,

    H. Lines under tension may be subjected to sudden shocks  or strains that cause them to bounce
       violently  up and down.  Never straddle or step  over a line or wire under  tension.   Be
       especially cautious  when the gear has become snagged or fouled.

    I.  Stay out from underneath loads suspended in the air, and keep in mind that suspended loads
       become battering rams in rolling  seas.  A tag line must be attached to any load being hoisted
       to control turning and swinging.

    J.  Do  not stand between a  load in the  air and a  rail, stanchion, hatch coaming or any solid
       object against which you could be crushed.  If you are the man at the controls, never pass a
       load over another crewman.

    K. Be sure to use adequate lighting when working  in poorly lit areas  or during the night.

    L. Only lower one device overboard at a time unless  prior  approval has been obtained from the
       Captain or Officer  in Charge.

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                                                           SECTION:  APPENDIX K
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE-  15 of 17

    M.  Do not work alone on  the weather decks.  A crew  member with a radio  should be present
        during work at a sampling station.

    N.  Report any equipment failure or accident to the Bridge immediately.
6.0 Communication

    A. Clear communication between the  pilot house and deck should be maintained by means of
       properly located, functioning intercoms or radios.

    B. Position the ship's internal video system  in such a way  that the pilot  house can  view
       overboard  activities.

    C. Inform  pilot house of readiness to  sample or the presence of overboard gear or  lines in the
       water.

    D. Inform  curious onlookers of unsafe conditions.

    E. Inform  pilot house and clear decks after  sampling is complete.   Secure  science and  deck
       gear. Turn off all electrical and mechanical equipment.

    F. When verbal communication is impossible or likely to  be interfered with by other noises, the
       crew should utilize a standard set of hand signals.  Where  the winch operator does not have
       a clear  view of the load, only one  designated person will be responsible for  giving the hand
       signals.
7.0 Deck Machinery

    A.  Gear systems, including wrapping heads, winches, tackle, nets, etc.,  should provide safe and
        convenient operation.

    B.  Wires and wraps should be of adequate strength for the anticipated loads.

    C.  Moving parts of winches and of wrap and chain  leads which may  present a hazard should
        have adequate guards.

    D.  Repairs to winches, tackle and  lifting gear should be to original standards of construction.
        Repairs should be tested using dead loads before the gear is placed back in service.

    F,.  Protection should be  provided  around winch  foundations to prevent a person from being
        caught or dragged under.

    F.  Sheaves  should  be  guarded where   possible.   Blocks  and  sheaves  should be properly
        lubricated at regular intervals.

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                                                           SECTION:  APPENDIX K
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE.  16  of 17

    G. All shackles used aloft  should be safety  wired or "moused" so they  cannot  come loose
       accidentally.

    H. Winches should  be  provided with a  means to  prevent over  hoisting and  to  prevent  the
       accidental release of a load if the power supply  fails.
8.0 General Lifting Precautions

    A. Components must  be  of sufficient  size to accomplish  the  task, and should  be inspected
       regularly.

    B. Fiber lines should be checked for signs of excessive wear, fraying, rot and dryness.

    C. Wire rope should be examined for fish hooks, badly worn areas and kinks.  Badly worn wire
       ropes should be replaced.

    D. Wooden blocks should be  inspected for cracked or rotten  cheeks, worn  pins or  cracked  or
       badly worn  metal parts.

    E. Shackles, swivels,  metal  blocks  and  hooks  should  be inspected  for  cracks,  distortion,
       excessive wear or metal fatigue.

    F. If one part of a lifting  appliance gives way, the force may weaken or destroy other parts.

    G. Heavy weights should  never be permitted to drop regardless of the distance.  They should be
       lowered to rest and secured to  prevent  rolling or sliding. Never keep a  load in the air any
       longer than  necessary.

    H. All motions with heavy weights should be slow to avoid creating momentum.  Attaching a
       tag line will help avoid many problems and ensure  safe handling of the load.

    I.  Avoid sudden  shocks or strain,  and  beware of side pulls.  Side play puts great stress on a
       boom or crane.

    J.  Only certified crane operators are permitted to operate the ship's cranes and hoists.

    K. Any  person directing  the  crane operator must be trained  in the use of standard operating
       hand signals.  (See  Attachment  A)

    L. Report any  equipment  failure or accident to the Bridge immediately.

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STANDARD OPERATING PROCEDURE


Section
1 0
20
3 0
40
50
6.0
70
1
90
10.0
11.0
120
13 0
140
15 0
Prepared by
Approved by
Rev
Operational Safety Practices During Use of H1AB
Aboard the R/V Lake Guardian
Table of Contents
Title
Scope
References
Responsibility
Inspection
Testing
Maintenance
Adjustments and Repairs
Rope) Inspection
Rope Replacement
Rope Maintenance
Operation
Handling & Moving the Load
Holding the Load
Signals
Miscellaneous
Date
Date

Crane

Page
9
2
2
2-3
3-4
4
4-5
6
6-7
7
7-9
9-10
10
10
11

            Page 1

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                       Operational Safety Practices During Use of HIAB Crane

 1 0    Scope

 To provide standard operating procedures for use before, during and after operation of the HIAB crane.  These
 procedures shall be followed by all personnel aboard the R/V Lake Guardian involved wi:h the handling, maintenance
 and operation of the HIAB crane

 2.0    References

 Crane Inspection & Certification Bureau (CICB) Training Manual:  Mobile Cranes. & Rigging Considerations.
 Chapter 1-2.

 The contents of this document are based on recognized Safety Standards, which are published by the following
 Organizations or Agencies:

 AMERICAN NATIONAL STANDARDS INSTITUTE
 OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION
 SOCIETY OF AUTOMOTIVE ENGINEERS
 POWER CRANE AND SHOVEL ASSOCIATION
 AMERICAN WELDING SOCIETY
 WIRE ROPE INSTITUTE OF AMERICA
CONSTRUCTION SAFETY ASSOCIATION OF ONTARIO

 3 0    Responsibility
 There are several CICB certified mobile crane  operators aboard the R/V Lake Guardian who will assume primary
 responsibility for handling, maintenance and operation of the HIAB crane.

 4.0    Inspection

 Items such as the following shall be for  defects at  monthly intervals,  or as specifically indicated, including
 observation during operation for any defects which might appear between regular inspections. Any deficiencies such
 as listed shall be carefully examined and determinations made as to whether they constitute a safety hazard:

       A    Prior to initial use all new  and/or altered cranes shall be inspected to insure compliance with
             provisions contained in Crane Inspection and Certification Manual.

       B.    All control mechanisms shall be inspected for maladjustments interfering with proper operation. Daily

       C.    All control mechanisms shall be inspected for excessive wear of components and contamination by
             lubricants or other foreign matter.

       D.    All safety devices shall be inspected for malfunction.
                                              Page 2

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       E      Inspections for deterioration or leakage in air or hydraulic systems. Daily

       F      Crane hooks shall be  inspected for deformations, cracks or having  more than 15% in excess of
              normal throat opening or more than 10 degree twist from the plane of the unbent hook

       G      Inspections  are  required  for  rope  reeving  for  noncompliance  with  crane  manufacturer's
              recommendations

       H.     Electrical apparatus shall be inspected weekly for malfunctioning, signs of excessive deterioration,
              din and moisture accumulation

A complete inspection of the crane shall be performed on a yearly basis. This inspection shall include all of the
requirements above as well as the following items  Any deficiencies such as  listed shall be carefully examined and
a determination made as to whether they constitute a safety hazard.

       I.      Deformed, cracked or corroded members in the crane structure and boom

       J.      Loose bolts or rivets.

       K.     Cracked or worn sheaves and drums.

       L.      Load, boom angle and other indicators over their full range, for any significant inaccuracies.

       M.     Gasoline, diesel, electric or other power plants for improper performance or noncompliance with
              safety requirements.

       N.     Crane hooks.  Magnetic particle or other suitable crack detecting inspection should be performed
              at least once each year.

A crane which has been idle for a period of one month or more, but less than six months, shall be given an inspection
conforming with requirements of the weekly inspection above, before placing in service

A crane which has been idle for a period of over six months shall be given a complete inspection conforming with
requirements of the weekly and yearly inspections, before placing in service.
50
       A.     Prior to initial use all extensively repaired or altered cranes should be tested by or under the direction
              of an appointed or authorized person.

       B.     Written reports shall be furnished by such persons showing test procedures  and confirming the
              adequacy of repairs or alterations.
                                                Page 3

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       C      Test loads shall not exceed 110% of the rated load at any selected woiking radius.

       D      Re-rating test report shall be readily available

       E.      No cranes shall be re-rated in excess of the original  load ratings unless such rating changes are
              approved by the crane manufacturer or final assembler.

6.0    Maintenance

       A.     A preventative maintenance program based on the crane manufacturer's recommendations shall be
              established.  Dated and detailed records shall be readily available.

       B,     It is recommended that replacement parts be obtained from the original equipment manufacturer.

       C.     Before adjustments and repairs are started on a erne the following precautions shall b- taken as
              applicable.

              (1)  Crane placed where it will cause the least interference with other equipment or operations in
                  the area.

              (2)  All controls in the "OFF" position.

              (3)  Starting means rendered inoperative.                                                                  ^m

              (4)  Warning or "out of order" signs placed on the crane.

              (5)  Power plant stopped or disconnected at take-off.

              (6)  Boom lowered to the ground if possible or otherwise secured against dropping.

              (7)  Lower load block lowered to ground or otherwise secured against dropping.

       D.     After adjustments and repairs have been made the crane shall not be operated until all guards have
              been reinstalled, safety devices reactivated  and maintenance equipment removed.

7 0    Adjustments and Repairs

       A.     Any unsafe conditions disclosed by the inspection shall  be corrected before operation of the crane is
              resumed.  Adjustments and repairs shall be done only  by designated personnel.

       B.     Adjustments shall be maintained to assure correct  functioning  of components. (All  functional
              operating mechanisms, safety devices, control systems and power plants).


                                                Page 4

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       C.     Repairs or replacement •shall be provided promptly as needed for safe operation

       D.     All replacement parts or repairs shall have at least the original safety factor

       E.     All moving parts of the crane for which lubrication is specified, including rope and chain, shall be
              regularly lubricated  Lubricating s\ stems shall be checked for proper delivery of lubricant

              Particular care should be taken to follow manufacturer's recommendations as to points and frequency
              of lubrication, maintenance of lubricant levels and types of lubricant to be used.

       F.     Machinery shall be stationary while lubricants are being applied and protection provided as called for
              in the above section "Maintenance" C-l  through C-7, unless equipped  for automatic  lubrication

8.0    Rope Inspection

       A.     All running ropes in continuous service should be visually inspected once every working day.  A
              thorough inspection of all rope in use shall be made at least once a month and a full written, dated
              and signed report of rope conditions kept on file where readily available.  All inspections shall be
              performed by an appointed or authorized person.  Any deteriorations, resulting in appreciable loss
              of original strength, such as described below, shall be carefully noted  and determinations made as to
              whether further use  of the rope would constitute a safety hazard
              (1)  Reduction  of rope diameter below nominal diameter due to loss of core support, internal or
                 external corrosion or wear of outside wires.

              (2)  A number of broken outside wires and the degree of distribution of concentration of such
                 broken wires.

              (3)  Worn outside wires

              (4)  Corroded or broken wires at end connections.

              (5)  Corroded, cracked, bent, worn or improperly applied end connections.

              (6)  Severe kinking,  crushing, cutting or outstanding.

       B     Heavy wear and/or  broken wires may occur in sections in contact with equalizer sheaves or other
              sheaves where rope travel is limited, or with saddles.  Particular care shall be taken to  inspect ropes
              at these locations.
                                                PageS

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       C      All rope which has been idle for a period of a month or more due to shut down or storage of a crane
              on which it is  installed shall be given a thorough inspection before it is placed in service   This
              inspection shall  be for all types of deterioration and shall be performed by an appointed or authorized
              person whose approval  shall be required for further use of the rope  A written and dated report of
              the rope condition shall be filed

       D.     Particular care shall be taken in the inspection of non-rotating rope

9 0    Rope Replacement

       A.     No precise rules can be given for determination of the exact time for replacement of rope, since many
              variable factors are involved. Safety in this respect depends largely upon the use of good judgement
              by an appointed or authorization person in  evaluating remaining strength in  a used rope after
              allowance for  deterioration disclosed by inspection   Safety of rope operation depends upon this
              remaining strength.

       B.     Conditions such as the following should be sufficient reason  for questioning rope safety and for
              consideration of replacement.

              (1)  In running ropes, six randomly distributed broken wires  in one lay or three broken wires in
                  one strand in one lay.

              (2)  Wear of one-third the original diameter of outside individual wires.

              (3)  Kinking, crushing, bird caging or any other damage resulting in distortion of the rope
                  structure.

              (4)  Evidence of any heat damage from any cause.

              (5)  Reductions from nominal diameter of more than:
                            3/64" for diameters to and including 3/4"
                            1/16" for diameter 7/8" to 1-1/8" inclusive
                            3/32" for diameters 1-1/4" to 1-1/2" inclusive

              (6)  In standing ropes,  more than two broken wires in one lay in sections beyond end connections
                  or more than one broken wire at an end connection.

       C.     In order to establish data as a basis of judging the proper  time  for replacement,  a continuing
              inspection record shall be maintained  This record  shall cover points of deterioration listed in
              "inspection" of this section
                                                 Page 6

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10 0    Rope Maintenance

       A      Rope shall be stored to prevent damage or deterioration

       B.      Unreeling or uncoiling of rope shall be done as recommended by the rope manufacturer and with
              extreme care to avoid kinking or inducing a twist

       C      Before cutting a rope, seizing shall be placed on each side of the place where the rope is to be cut to
              prevent unlaying of the strands  On preformed rope, one seizing on each side of the cut is required
              On  non-preformed  ropes of 7/8" diameter or smaller, two seizings  on each side of the cut  are
              required, and for non-preformed rope of one inch diameter or larger, three seizings on each side of
              the cut are required.

       D.      During installation care shall be observed to avoid dragging of the rope in dirt or around objects
              which will scrape, nick, crush or induce sharp bends in it.

       E.      Rope should be maintained in a well lubricated condition It is important that lubricant applied as part
              of a maintenance program shall be compatible with the original lubricant and to this end the rope
              manufacturer should be consulted.   Those sections of rope which are located  over sheaves or
              otherwise hidden during inspection and  maintenance procedures require  special attention  when
              lubricating rope  The object of rope lubrication is to reduce internal friction and to prevent corrosion.
              Periodic field lubrication is particularly important for non-rotating rope

       F.      When an operating rope shows greater wear at well defined localized areas than on  the remainder of
              the rope, rope life can be extended in cases where a reduced rope length is adequate by cutting off
              a section at one end, and thus shifting the wear to different areas of the rope.

11.0    Operation

       A      Cranes shall only be operated by designated operators, learners under the direct supervision of a
              designated operator, maintenance and test personnel when it is necessary in the performance of their
              duties, and inspectors.

       B.      No one, other than  personnel specified in the above paragraph shall enter a crane cab, with  the
              exceptions of persons such as oilers and supervisors, whose duties require them to Jo so, and then
              only in the performance of their duties and with the knowledge of the operator or other person.

       C.      Operators shall be required to  pass a practical operating examination. Examinations shall  be limited
              to the specific type equipment which he will operate.

       D.      Operators shall meet the following physical qualifications.

              (1)     Have vision of at least 20/30 Snellen in one eye,  and 20/50 in the other, with or without
                     glasses
                                                 Page 7

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       (2)    Be able  to  distinguish  red, green, and yellow regardless of position of colors,  if color
              differentiation is required for operation

       (3)    Hearing, with or without hearing aid, must be adequate for the specific operation

       (4)    A history of epilepsy or of a disabling heart condition shall be sufficient reason for his
              disqualification.

E.     The operator shall not engage in any practice which will divert his attention while actually engaged
       in operating the crane.

F.     When he is  physically or mentally unfit, an operator shall not engage in  the  operation of this
       equipment.

G.     The operator shall respond to signals only from the appointed signal man, but shall obey a stop  signal
       at any time, no matter who gives it.

H.     If a warning signal is furnished, it shall be sounded each time before traveling, and intermittently
       during travel, particularly when approaching workmen.

I.      Before leaving his crane unattended, the operator shall:
       (1) Land any attached load, bucket, lifting magnet or other device.
       (2) Crane booms shall be lowered to ground level or otherwise
           fastened securely against displacement by wind loads or other outside forces.
       (3) Set travel, swing, boom brakes and other locking devices.
       (4) Put controls in the "OFF" position.
       (5) Stop the motor.

J.     If there is a warning sign on the switch or motor starting controls, the operator shall not close the
       switch or start the motor until the warning sign has been removed by the person placing it there.

K.     Before closing the  switch or starting the motor, the operator shall see  that all controls are in the
       "OFF" position and all personnel are in the clear.

L     If power fails during operation, the operator shall set all brakes and locking devices, move all power
       controls to the  "OFF" position and if practical, the suspended load should be landed under brake
       control.
                                           PageS

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      M     The operator shall familiarize himself with the equipment and its proper care   If adjustments or
             repairs are necessary, or any defects are known, he shall report the same promptly to the appointed
             person and shall also notify' the next operator of the defects upon changing shifts

      N     Booms v«hich are being assembled or disassembled on the ground with or without support of the
             boom harness should be securely blocked to prevent dropping of the boom and boom sections

12 0   Handling & Moving the Load

      A     No crane shall be loaded beyond the rated load, except for test purposes

      B.     When loads which are limited by their own  structural integrity  rather than by stability are to  be
             handled, the person responsible for the job  shall ascertain that  the weight of the load has been
             determined within plus or minus 10% before it is lifted.

      C     The hoist rope shall not be wrapped around the load.

      D.     The load shall be attached to the hook by means of slings or other approved devices.

      E.     The individual directing the lift shall see that the crane is level and that  the load is well secured and
             properly balanced in the sling or lifting device before it is lifted more than a few inches

      F.     Before starting the hoist, note the following conditions:

             (1)    Hoist rope shall not be kinked.
             (2)    Multiple part lines shall not be twisted around each other.
             (3)    The hook shall be brought over the load in such a manner as to prevent swinging.
             (4)    If there is a slack rope condition, it should be determined that the rope is properly seated on
                    the drum and in the sheaves.

      G     During hoisting care shall be taken that there is no sudden acceleration of deceleration of the moving
             load and that the load does not contact any obstructions.

      H.     Side loading of booms shall  be limited to freely suspended loads.  Cranes shall not be used for
             dragging loads sideways.

      I.     The operator shall not hoist, lower, swing or travel while anyone  is on  the load or hook.

      J.     The operator should avoid carrying loads over people.

      K.     The operator shall test the preset hydraulic lifting limit each time a load approaching the rated load
             is handled by raising it a few inches and observing it's ability to  hold the load in position without
             lowering.

                                                Page 9

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                                                                                                                      4
      L     Neither the load nor the booms shall be lowered below the point where less that two full wraps or
             rope remain in their respective drums.

      M.    In transit the following additional precautions shall be exercised:
                    (1)    The boom shall be carried in line with the direction of motion.
                    (2)    The empty hook shall be lashed or otherwise restrained so that it cannot swing freely

      N     When rotating the crane, sudden starts and stops shall be avoided. Rotational speed shall be such that
             the load does not swing out beyond the radii at which it can be controlled.  A tag or restrain line shall
             be used when rotation of the load is hazardous.

      O.    Ropes shall not be handled on a winch head without the knowledge of the operator.

      P.     While a winch head is being used, the operator shall be within convenient reach of the power unit
             control level.

13.0  Holding a Load

      A.    The operator shall not leave his position at the controls while the load is suspended.

      B.    No person should be permitted to stand or pass under a load on the hook.

       C.    If the load must remain suspended for any considerable length of time,  the operator shall hold the
             drum from rotating in the lowering direction by  activating the positive controllable means at the
             operators station.
140
       A.     Standard signals to the operator shall be in accordance with the standards prescribed in the next
              paragraph, unless voice communication equipment (telephone, radio or equivalent) is utilized.  Signals
              shall be discernible or  audible at all times.  No response shall be made unless signals are clearly
              understood.

       B.     Hand signals shall be in accordance with Attachment A.

       C.     For operations not covered by the hand signals, or for special conditions that occur from time to time,
              additions to or modification of the standard signals may be required.  In such cases these special
              signals shall be agreed on in advance by the operator and the signalman and should not be in conflict
              with standard signals.

                                                Page 10

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       D.     If it is desired to give instructions to the operator, other than provided by the established signal
              system, the crane motions shall be stopped

15.0   Miscellaneous

       A.     Except where the electrical distribution and transmission lines have been de-energized and visibly
              grounded at point of work or where insulating barriers not a part of or an attachment to the crane
              have been erected to prevent physical contact with the lines, cranes shall be operated proximate to,
              under, over, by or near power lines only in accordance with the following:

              (1)     For lines rated 50Kv or below, minimum clearance between the lines and any part of the crane
                     or load shall be 10 feet.

              (2)     For lines rated over 50kV minimum, clearance between the lines and any part of the crane or
                     load shall be 10 feet plus 0 4 inch for each IkV over 50kV, or use twice the length of the line
                     insulator but never less than 10 feet.

              (3)     It is recommended that a person be designated to observe the clearance and give timely
                     warning for all operations where it is difficult for the  operator to maintain the desired
                     clearance by visual means.

       B.     Cage-type boom guards, insulating lines, or proximately warning devices may be used on cranes, but
              the use of such devices shall not operate to alter the requirements of paragraph (A) above,  even if
              such devices are required by law or regulation

       C.     Before the commencement of operations near electrical lines, the person responsible for the job shall
              notify the owners of the lines or their authorized representative providing them with all   pertinent
              information and requesting their cooperation

       D.     Any overhead wire shall be considered to be an energized line unless and until the person owning such
              line or the electrical utility authorities indicate  that it is not an energized line.

       E.     Exceptions to this procedure, if approved by the owner of the electrical  lines, may be granted by the
              administrative or regulatory authority if the alternate procedure provides sufficient protection and is
              set forth  in  writing

       F.     A carbon-dioxide, dry chemical or equivalent fire extinguisher shall be kept in the vicinity of the
              crane.  Operating and maintenance personnel shall be familiar with the use and care of the fire
              extinguishers provided

                                                 Page  11

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                      STANDARD OPERATING PROCEDURE

                        Deck Safety Practices During Use of
                     Scientific Equipment, A-Frames and Hoists
                                 Table of Contents
Section
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
Title
Scope
References
Responsibility
Frequency of Inspection
Deck Skills and
Safety Procedures
Communication
Deck Machinery
General Lifting Precautions
Page
2
2
2
2
3-4
4
5
5-6
                              Attachment A - Hand Signals
Prepared by


Approved by


Rev.
Date
Date
                                     Page 1

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                           Deck Safety Practices During Use of
            Overboard Science Equipment, A-Frames, HIAB Crane and Hoists
1.0    Scope

To provide standard operating procedures and general deck safety practices during the use of
overboard science equipment, A-Frames, HIAB Crane, Hoists or any other systems that lift, lower,
secure or otherwise handle objects on board the R/V Lake Guardian.

2.0    References

Environmental Protection Agency: GLNPO Chemical Hygiene Plan and Safety Manual. Page 34.

Environmental Protection Agency, Region 5:  Heaith and Safety Manual. Section 18-1.

North Pacific Fishing Vessel Owner's Association:  Vessel Safety Manual. Chapter 4, "Working
Conditions".

Seaward Services, Inc.: Occupational Safety & Health Program for Employees Working Aboard the
R/V Roger R. Simons and R/V Lake Guardian. Section 2-1.

3.0    Responsibility

The ship's Master is responsible for implementation and accomplishment of the procedures contained
herein.

4.0    Frequency of Inspection

Prior to the use of any piece of lifting gear, crane, A-frame, davit or other handling device, either the
ship's Science Officer, Marine Science Technician, the mate on watch or appointed designee, shall
visually inspect the equipment involved with the operation and attest to its ability to perform the
intended operation.  This inspection shall include slings, hoists, blocks,  ropes and associated
hardware.  On an annual basis, preferably several weeks prior to commencement of a new operating
season, all of the aforementioned gear will be inspected by a qualified and independent third party for
material condition and safe working load.  Straps, chains, shackles, turnbuckles, etc.,  shall be
condemned and removed from the vessel if found to be unserviceable.  Cranes, winches, A-frames
and davits found to be unserviceable shall be tagged "Out of Order" and repaired prior to further use.
                                         Page 2

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5.0    Deck Skills and Safety Procedures

       A.     Work vests shall be worn at all times during sampling and testing conducted on the
              weather decks.

       B.     Hard hats must be worn by crane operators and any persons assisting with the load.

       C.     All individuals involved in sampling procedures on deck shall wear appropriate safety
              ^ear.  (Life vests, work suits, hard hats, eye, face,  hand and foot protection, safety
              lines etc..).  Employees will be provided with personal protective equipment and
              trained on the use and care of this equipment.  In compliance with OSHA 1910
              standards, vessel employees are required to adhere to any and all policies regarding
              personal  protective equipment for their own well being.

       D.     No one  is permitted on the weather decks during sea operations  unless directly
              involved  with sampling, testing, checking of equipment or other work.

       E.     During weather conditions causing freezing of water on deck surfaces, hoses shall be
              used to thaw surfaces with  warm water as needed to prevent a slipping hazard for
              those working outside. Special precautions are necessary in wet, slippery conditions.

       F,     Never stand in a bight (a loop of line, rope or chain) or put any part of your body into
              a bight without first making sure that the gear cannot be worked.

       G.     Never stand in the  direct line of pull when a  rope or wire is under tension, in case it
              breaks and snaps back. Generally, rope or wire snaps back with a corkscrew motion,
              so the danger zone extends to either side of the direct line of pull.

       H.     Lines under tension may be subjected to sudden shocks or strains that cause them to
              bounce violently up and down.  Never straddle or step over a line or wire under
              tension.  Be especially cautious when the gear has become snagged or fouled.

       I.      Stay out from underneath loads suspended in the air, and keep in mind that suspended
              loads become battering rams in rolling seas. A tag line must be attached to any load
              being hoisted to control turning and swinging.
                                          Page 3

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       J.      Don't stand between a load in the air and a rail, stanchion, hatch coaming or any solid
              object against which you could be crushed.  If you're the man at the controls, never
              pass a load over another crewman.

       K.     Be sure to use adequate lighting when working in poorly lit areas or during the night.

       L.     Only lower one device overboard at a time unless prior approval has been obtained
              from the Science Officer.

       M.     Do not work alone on the weather decks. A crew member with a radio should be
              present during work at a sampling station.

       N.     Report any equipment failure or accident to the Bridge immediately.
6.0    Communication

       A.     Clear communications between the pilot house and deck should be maintained by
              means of properly located, functioning intercoms or radios.

       B.     Position the ship's internal video system such that the pilot house can view overboard
              activities.

       C.     Inform pilot house of readiness to sample or the presence of overboard gear or lines
              in the water.

       D.     Inform curious onlookers of unsafe conditions.

       E.     Inform pilot house and clear decks after sampling is complete. Secure science and deck
              gear. Turn off all electrical and mechanical equipment.

       F.     When verbal communications are impossible or are likely to be interfered with by
              other noises, the crew should utilize a standard set of hand signals. Where the winch
              operator doesn't  have a clear view of the load, one designated person will be
              responsible for giving the hand signals, not the whole crew.
                                         Page 4

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7.0    Deck Machinery

       A.     Gear systems, including wrapping heads, winches, tackle, nets, etc., should provide
              safe and convenient operation.

       B.     Wires and wraps should be of adequate strength for the anticipated loads.

       C.     Moving parts of winches and of wrap  and chain leads which may present a hazard
              should have adequate guards.

       D.     Repairs to winches, tackle  and lifting  gear should  be to original standards of
              construction. Repairs  should be tested using dead loads before the gear is placed
              back in service.

       E.     Protection should be provided  around winch foundations toprevent a person from
              being caught or dragged under.

       F.     Sheaves should be guarded where possible. Blocks and sheaves should be properly
              lubricated at regular intervals.

       G.     All shackles used aloft should be safety wired or "moused"  so they cannot come loose
              accidentally.

       H.     Winches should be provided with a means to prevent over hoisting and to prevent the
              accidental release of a load if the power supply fails.

8.0    General Lifting Precautions

       A.     Components must be of sufficient size to accomplish the task, and should be inspected
              regularly.

       B.     Fiber lines should be checked for signs of excessive wear, fraying,  rot and dryness.

       C.     Wire rope should be examined for fish hooks, badly worn areas and kinks. Badly
              worn wire ropes should be replaced.

       D.     Wooden blocks  should be inspected for cracked  or rotten cheeks,  worn pins or
              cracked or badly worn metal parts.

       E.     Shackles, swivels, metal  blocks and hooks should be inspected for cracks,  distortion,
              excessive wear or metal fatigue.
                                          Page 5

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F.     If one part of a lifting appliance gives way, the force may weaken or destroy other
       parts.

G.     Heavy weights should never be allowed to drop regardless of the distance. They
       should be lowered to rest and secured to prevent rolling or sliding. Never keep a load
       in the air any longer than necessary.

H.     All motions with  heavy weights should be slow  to  avoid creating momentum.
        \ttaching a tag line will help avoid many problems and insure safe handling of the
       load.

I.      Avoid sudden shocks or strains, and beware of side pulls. Side play puts great stress
       on a boom or crane.

J.      Only certified crane operators are permitted to operate the ship's cranes and hoists.

K.     Any person directing the crane operator must use and be trained in the use of standard
       operating hand signals. (See attachment A).

L.     Report any equipment failure or accident to the Bridge  immediately.
                                   Page 6

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                                                    SECTION: APPENDIX L
                                                    VERSION:  FINAL/MAY 1997
                                                    PAGE: COVER
APPENDIX L:  CHEMICAL HYGIENE STANDARD OPERATING PROCEDURES FOR U.S. EPA GLNPO
            RESEARCH VESSELS

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Chemical
Hygiene
SOP No. :
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
Overall Glove
Recommendation
Optional
CPE*
CPE
CPE
CPE
CPE
CPE
Vinyl
CPE
CPE
Vinyl
CPE
CPE
CPE
CPE
CPE
CPE
Insulated
N/A
Vinyl
CPE
Laboratory Procedure
Dissolved Nutrients Filtration
' Total Alkalinity
Ammonia Nitrogen
Chloride
Dissolved Organic Carbon
Chlorophyll-a & Chlorophyll-b
Chlorophyll-a & Pheophytin-a
Specific Conductance
Nitrate & Nitrite Nitrogen
Dissolved Oxygen
Dissolved Oxygen
Electrometric pH
Soluble Reactive Phosphorous
Total Phosphorous, Total
Dissolved Phosphorous
Silicates
Standards & Spikes Preparation
Sulfate
Suspended Solids
Technicon Autoanalyzer &
Associated Recording Equip.
Turbidity
Aerobic Heterotrophs, Total
                                   Coliforms,  Fecal Coliforms,
                                   Fecal Streptococci
22
Neoprene
Determining Primary Production
Parameters

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Chemical
Hygiene
SOP No. :
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
Overall Glove
Recommendation
N/A
N/A
Neoprene
CPE
CPE
CPE
CPE
CPE
CPE
CPE
CPE
CPE
CPE
CPE
CPE
Laboratory Procedure
Quality Control Schedule
Reagent Water System
Sampling Surface Waters for
Hydrophobic Contaminants
Including Filtration, Liquid/
Liquid Extraction
Determination of Metals in
Acid Rain
Determination of Cadmium,
Chromium, & Nickel in Acid
Rain
Determination of Total Arsenic
in Water
Determination of Total Potassium
& Sodium in Acid Rain
Determination of Nickel in Acid
Rain
Analysis of Total Kjeldahl
Nitrogen & Total Phosphorous
in Water
Analysis of Particulate Organic
Carbon in Lake Water
Direct Observation of Bacteria
by DAP I
Analysis of Phytoplankton
Analysis of Zooplankton
Analysis of Total Phosphorous
Determination of Calcium,
"-—— — ,. 4.,™ D/-.4- a e a i urn £
Sodium

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Chemical
Hygiene
SOP No.;

38
39
Overall Glove
Recommendation

CPE
See Pg. 39-1
Laboratory Procedure

Determination of Total Calcium
Magnesium, Potassium, &
Sodium

Liquid/Liquid Water Extraction
Capture
*CPE = CHLORINATED POLYETHYLENE

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                                                               1-1
                        CHEMICAL HYGIENE SOP

                               No.  1
 Laboratory Procedure:    Dissolved Nutrients Filtration

 Method:                  	

 Description:             An unaltered sample is vacuum filtered
                          through a pre-washed selected .45 urn
                          membrane filter.

 Chemicals Used:          None

 Overall Glove Recommendation:  Gloves optional,  any protective
                                glove

 SAFETY CONTROLS

                                                   PPE and/or
 Procedure             Potential Exposure     Engineering  Controls

 Pouring sample into     None                   General ventilation
 separatory  funnel,                            is adequate.   PPE-
 draining,  shaking,                            General     lab
 filtering, etc.                                requirements,
                                               safety glasses, lab
                                               coat,  gloves   not
                                               required,    rubber
                                               gloves optional

WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.

SPECIAL PRECAUTIONS

None

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                                                              2-1
                        CHEMICAL HYGIENE SOP

                               No. 2
 Laboratory Procedure:

 Method:

 Description:


 Chemicals Used:
           Total Alkalinity

           Titration

           A measured amount of sample is titrated
           with acid to a pH of 4.5.

           Sulfuric acid, pH buffers,  sodium
           carbonate.
 Overall Glove Recommendation:   Chlorinated  polyethylene  (CPE)

 SAFETY CONTROLS
 Procedure

 1.   Sulfuric
 dilutions,
 necessary
acid
  if
Potential Exposure

 Spills,  splashing,
 inhalation
2. Stock alkalinity
standard
preparation ,
(sodium carbonate)
        None,  except with
        excessive    skin
        contact
3. Stock alkalinity
dilutions,  handling
        None
     PPE and/or
Engineering Controls

 General ventilation
 is  adequate.    If
 practical,    do
 procedure in a fume
 hood.    PPE-splash
 p»roof    safety
 goggles, lab  coat,
 viton,  chlorinated
 polyethylene,
 polyethylene gloves

 General ventilation
 is  adequate.    If
 practical,    do
 procedure in a fume
 hood.    PPE-splash
 proof     safety
 goggles, lab  coat,
 any    protective
 glove  or CPE

 General ventilation
 adequate.      PPE-
 safety glasses, lab
 coat,    gloves
 optional.   If used,
 any    protective
 glove  is adequate.
 CPE

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                                                              2-2
 Procedure

 4.  Handling of  pH
 buffers
Potential Exposure

 Spills,  splashing
 5.   Titration  of
 sample   of   check
 standard     with
 sulfuric acid
 Spills,  splashing,
 inhalation
     PPE and/or
Engineering Controls

  General ventilation
  is  adequate.   PPE-
  splash proof safety
  goggles,  lab  coat,
  gloves    not
  required,     butyl
  gloves optional.
  CPE

  General ventilation
  is  adequate.     If
  practical,    do
  procedure in a fume
  hood.     Sulfuric
 acid concentrations
 are  low.     PPE-
 splash proof safety
 goggles,  lab coat,
 viton,  chlorinated
 polyethylene,
 polyethylene gloves
 optional,    not
 required
WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.

SPECIAL PRECAUTIONS
Thoroughly wash hands after procedure completion.

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                                                              3-1
                       CHEMICAL HYGIENE SOP

                               No.  3
 Laboratory Procedure:

 Method:


 Description:


 Chemicals  Used:
   Ammonia Nitrogen

   Automated phenolate/nitroprusside
   spectrophotometric determination

   Ammo concentration of a sample determined
   spectrophotometrically.

   Sodium hydroxide phenol,  5.25% chlorine
   bleach,  potassium sodium tartrate, sodium
   citrate, sulfuric acid, Brij-35,  sodium
   nitroprusside,  ammonia standard
Overall Glove Recommendation:  Chlorinated Polyethylene (CPE)
SAFETY CONTROLS
Procedure

1. Sodium phenolate
solution
preparation,  phenol
and    sodium
hydroxide
Potential Exposure

 Spills,  splashing,
 inhalation
     PPE and/or
Engineering Controls

  Do  procedure in  a
  fume hood.  When in
  contact with water,
  sodium    hydroxide
  may    generate
  sufficient  heat  to
  ignite     a
  combustible
  materials.   Allow
  to  cool.     PPE-
  splash proof safety
  goggles, lab coat,
  butyl     gloves
  (choice),    viton
  gloves  (adequate)
  for    phenol,
  nitrile,  neoprene
  or  rubber  gloves
  (choice),    butyl
 gloves  (adequate)
 for    sodium
 hydroxide.      CPE
 adequate    for
 procedure

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                                                             3-2
 Procedure

 2 .     Sodium
 hypochlorite
 solution,    5.25%
 chlorine  bleach
Potential Exposure

 Spills,  inhalation
 3.     Complexing
 reagent,  potassium
 sodium   tartrate,
 sodium    citrate,
 sulfuric    acid,
 Brij-35
 Inhalation
4  .    Sodium
nitroprusside
5. Sodium hydroxide
Spills,  splashing,
inhalation
     PPE and/or
Engineering Controls

  General ventilation
  is adequate.    Do
  procedure in a fume
  hood  if  practical.
  PPE-splash    proof
  safety goggles, lab
  coat, rubber gloves
  or CPE

  General ventilation
  is adequate.    If
  practical,    do
  procedure in a fume
  hood.   PPE-splash
  proof     safety
  goggles,  lab coat,
  viton, chlorinated
  polyethylene,
  polyethylene gloves

  Sodium    nitro-
 prusside     is    a
 poison.     Perform
 procedure  in  fume
 hood.    PPE-splash
 proof    safety
 goggles,  lab coat,
 & rubber  gloves or
 CPE

 When   in   contact
 with water,  sodium
 hydroxide    may
 generate sufficient
 heat   to    ignite
 combustible
 materials.    Allow
 to   cool.       Do
 procedure in a fume
 hood.   PPE-splash
proof   safety
goggles,  lab coat,
nitrile,   neoprene
or  rubber  gloves
 (choice),     butyl
gloves    or    CPE
adequate

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                                                               3-3

                                                  PPE  and/or
Procedure              Potential Exposure     Engineering  Controls

6.     Standards     As  listed  in  SOP     As  listed  in  SOP
handling                No.  16                 No. 16.   Gloves  -
                                               CPE
WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Method
SPECIAL PRECAUTIONS

Use care  handling  sodium hydroxide which may generate  high heat
when in contact with water.

Wash hand thoroughly after procedure completion.

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                                                              4-1
                        CHEMICAL HYGIENE  SOP

                               No. 4

                          Chloride

                          Automated Ferricyanide Spectrophoto-
                          metric Determination

                          Liberated thiocyanate ion,  in the
                          presence of ferric  ion/  forms highly
                          colored ferric  thiocyanate.   The ferric
                          thiocyanate is  determined  spectrophoto-
                          metrically.

 Chemicals Used:           Chloride reagent, chloride  standards

 Overall Glove Recommendation:   Chlorinated Polyethylene  (CPE)
Laboratory Procedure:

Method:


Description:
 SAFETY CONTROLS

 Procedure
 1. Chloride reagent
 preparation,
 mercuric
 thiocyanate,
 methanol,    ferric
 nitrate,     nitric
 acid,  Brij-35
                      Potential  Exposure
                       Spills,  splashing,
                       inhalation,
                       flammable
2. Chloride reagent
handling
                      Slight inhalation
     PPE and/or
Engineering Controls
 This   material  is
 flammable    and
 hazardous.       Do
 procedure in a fume
 hood.    PPE-splash
 proof    safety
 goggles,  lab  coat,
 no  choice  gloves
 based  on data  for
 all    these
 materials .
 Recommend viton  or
 butyl  gloves  and
 double gloving. CPE
 adequate.      All
 reagents should  be
 covered and sealed
 with parafilm  when
 complete

 Concentrations are
 low,     general
 ventilation
 adequate.     PPE-
 splash proof safety
 glasses,  lab coat,
 gloving    not
 required,    viton,
 butyl or CPE gloves
 optional

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                                                              4-2
                                                    PPE and/or
 Procedure             Potential Exposure     Engineering Controls

 3.    Standards     As outlined in SOP     As outlined  in SOP
 handling               No. 16                 No.  16.   Gloves  -
                                               CPE
WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.
SPECIAL PRECAUTIONS

Keep ignition sources away from flammables. Keep containers closed
when not in use.

Wash hands thoroughly after procedure completion.

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                                                               5-1
 Laboratory Procedure:

 Method:

 Description:
                       CHEMICAL HYGIENE SOP

                               No.  5

                          Dissolved Organic Carbon
 Chemicals Used:
                          The determination of organic carbon
                          requires the removal of inorganic carbon,
                          Measurements are made with a non-
                          dispersive infrared analyzon.

                          Sulfuric acid, potassium persulfate,
                          organic carbon standard.
Overall Glove Recommendation:

SAFETY CONTROLS
                                Chlorinated Polyethylene (CPE)
 Procedure
 1.   Sulfuric  acid
 solution
 preparation ,
 handling
                      Potential Exposure
                       Spills,  splashing,
                       inhalation
2.     Persulfate
reagents, potassium
persulfate
3 .
      Standards
handling
As outlined in SOP
No. 19
     PPE and/or
Engineering Controls
  Do  procedure  in  a
  fume   hood.     Add
  acid  slowly.   PPE-
  splash proof safety
  goggles,  lab  coat,
  viton,  chlorinated
  polyethylene,
  polyethylene gloves

  Local   exhaust  is
  adequate.     PPE-
  splash proof safety
  goggles,  lab  coat,
  rubber  or  neoprene
  gloves.     CPE
  adequate.  Potassium
 persulfate   is  a
 strong    oxidizer.
 Keep    from
 flammables.

 As outlined in SOP
 119.   Gloves - CPE

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 Procedure

 4.    CO2   analyzer
 operation
Potential Exposure

 Compressed    gas
 cylinder explosions
 5.     Analytical
 system
 Dependent    on
 reagent or standard
 used
WASTE GENERATION AND HANDLING
                 5-2
     PPE and/or
Engineering Controls

  Keep compressed gas
  cylinders    secure
  and    upright.
  Follow    safety
  procedures    in
  Compressed    Gas
  Association
  Pamphlet P-l

  Follow   PPE   and
  Engineering
 Controls     as
 outlined  for  each
 reagent or standard
 used.   Gloves - CPE
Follow all guidelines of the  U.S. EPA's Characterization of Waste
from Standard Analytical Methods.

SPECIAL PRECAUTIONS

Compressed gas cylinders.

Thoroughly wash hands after procedure completion,.

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                                                               6-1
                        CHEMICAL HYGIENE SOP

                                No.  6
  Laboratory Procedure:

  Method:

  Description:
                          Chlorophyll-a and chlorophyll-b

                          Spectroflourometric Determination

                          Algae samples are steeped in ethanol.
                          The decrease in fluorescence at particular
                          wave  lengths  resulting from the acidi-
                          fication of the sample is proportional
                          to chlorophyll-a and chlorophyll-b

                          Acetone, sodium bicarbonate, chlorophyll-a
                          standard,  chlorophyll-b  standard,  mag-
                          nesium carbonate,  hydrochloric  acid,
                          acetone.

Overall  Glove  Recommendation:   Chlorinated  Polyethylene  (CPE)

SAFETY CONTROLS
 Chemicals Used:
Procedure

1. Acetone solution
preparation,
acetone and  sodium
bicarbonate
                       Potential Exposure

                        Spills, splashing,
                        inhalation ,
                        flammable
2.   Chlorophyll-a,
chlorophyll-b
standards
3.     Magnesium
carbonate
suspension
                       Spills, splashing,
                       inhalation
                       None
     PPE and/or
Engineer in g C on tro1s

  Acetone    is
  flammable.       Do
  procedure in a fume
  hood.    Keep  from
  ignition   sources.
  PPE-splash    proof
  safety goggles, lab
  coat, butyl, or CPE
  gloves

  Perform  procedure
  in fume hood.  PPE-
  splash proof safety
 goggles,  lab  coat
 CPE   or  neoprene
 gloves

 General ventilation
 is adequate.  PPE-
 splash proof safety
 glasses, lab coat

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 Procedure

 4.    Hydrochloric
 acid    solution
 preparation
 5.    Calibration,
 dilution    of
 chlorophyll
 standards    with
 acetone
6.    Analytical
procedure  ,
filtration

7.    Spectro-
fluorometer
preparation    and
procedure
                        Potential  Exposure

                         Spills,  splashing,
                         inhalation
                        Flammable
                        Flammable
                        Spills, inhalation,
                        flammable
                6-2
     PPE and/or
Engineering Controls

  Do procedure  in  a
  fume  hood.    PPE-
  splash proof safety
  goggles,  lab coat,
  rubber     gloves
  (choice),  viton  or
  CPE    gloves
  (adequate)

 Acetone and ethanol
  is   extremely
  flammable.       Do
 procedure in a fume
 hood.    Keep  from
 ignition   sources.
 PPE-splash    proof
 safety goggles, lab
 coat,   gloves  not
 required,     CPE
 gloves optional

 PPE- safety glasses
 arid lab coat
                                              PPE-safety  glasses
                                              and lab coat
WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S.  EPA's Characterization of Waste
From Standard Analytical Methods.

SPECIAL PRECAUTIONS
Use care in handling flammables
in use.
                                  Keep  containers closed when not


Wash hands thoroughly after procedure completion.

-------
                                                               7-1
 Laboratory Procedure:

 Method:

 Description:



 Chemicals Used:
 CHEMICAL HYGIENE  SOP

        No. 7

   Chlorophyll-a and Pheophytin-a

   Spectrofluorometric Determination

   Algae samples are steeped in acetone.
   Fluorescence at prescribed wavelengths is
   measured before and after acidification.

   Acetone, magnesium carbonate, chlorophyll-
   a standard,  hydrochloric acid.
 Overall Glove Recommendation:  Chlorinated Polyethylene (CPE)
 SAFETY CONTROLS
 Procedure

 1. Acetone solution
 preparation
Potential Exposure

 Spills,  splashing,
 inhalation
2 . Chlorophyll
standards
3.    Magnesium
carbonate
suspension
 Spills,  splashing,
 inhalation
None
     PPE and/or
Engineering Controls

 Acetone    is
 flammable.       Do
 procedure in a fume
 hood.    Keep  from
 ignition   sources.
 PPE-splash    proof
 safety goggles, lab
 coat, butyl  or CPE
 gloves

 Perform  procedure
 in fume hood.  PPE-
 splash proof safety
 goggles, & neoprene
 or CPE gloves

 General  ventilation
 is adequate.   PPE-
 splash proof safety
 glasses, lab coat,
 gloves     not
 required,     any
 protective    glove
 optional.       CPE
 gloves suggested

-------
pmredure

41    Hydrochloric
acid solution
                    Potential Exposure

                      Spills,  splashing,
                      inhalation
 5.    Calibrations,
 dilutions    of
 chlorophyll    with
 acetone
                       Flammable
 6.    Analytical
 procedure,
 filtration
                      Flammable
                        Spills, inhalation,
                        flammable
                7-2
     PPE and/or
Engineering Controls

  Do procedure  in a
  fume  hood.    PPE-
  splash proof safety
  goggles,  lab coat,
  rubber    gloves
  (choice), viton or
  CPE     gloves
  (adequate)

  Acetone     is
  flammable.    Keep
  from    ignition
  sources.         Do
  procedure in a fume
  hood.    PPE-splash
  proof     safety
  goggles,  lab  coat,
  CPE gloves

  Acetone    is
  flammable.     Keep
  from    ignition
  sources.        Do
  procedure in a fume
  hood.    PPE-splash
  proof    safety
  goggles, lab coat,
   butyl or CPE gloves
   optional

   Use PPE  as outlined
   for    handling
   reagents,     CPE
   gloves suggested
7 .     Spectro-
photometer    and
spectrofluorometer
procedures


WASTE GENERATION AND nn»~.	

Follow all guidelines in the U.S.  EPA's  Characterization  of Waste
From Standard Analytical Methods.

SPECIAL PRECAUTIONS

Use care  in  handling flammables.  Keep containers closed  when not

 in use.
 Wash  hands thoroughly after procedure completion.

-------
                                                               8-1
                        CHEMICAL HYGIENE SOP

                                No.  8
 Laboratory  Procedure:     Specific Conductance

 Method:                   Conductivity Bridge

 Description:              Specific conductance of water  samples
                           are measured by a self-contained con-
                           ductivity meter.

 Chemicals Used:           Sodium chloride, potassium chloride.

 Overall Glove Recommendation: Polyvinyl Chloride (Vinyl)

 SAFETY CONTROLS
 Procedure

 1.  Stock chloride
 set    standard
 solution,  working
 calibration
 standard
 preparation, sodium
 chloride

 2.   Stock  control
 standard,  control
 standard, potassium
 chloride
 3.    Calibration,
 heating of standard
Potential Exposure
 None
 None
Heat  (burns)
     PPE and/or
Engineering Controls

 General ventilation
 is  adequate.   PPE-
 safety glasses, lab
 coat,     any
 protective  gloves.
 Vinyl suggested
 General ventilation
 is adequate.   PPE-
 safety glasses, lab
 coat,     any
 protective   glove.
 Vinyl suggested

 Use local exhaust.
WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.

SPECIAL PRECAUTIONS

Wash hands thoroughly after procedure completion.

-------
                                                              9-1
 Laboratory Procedure;

 Method:


 Description:
                       CHEMICAL HYGIENE  SOP

                              No.   9

                         Nitrate and Nitrite Nitrogen

                         Cadmium reduction,  automated,  spectro-
                         photometric

                         Buffered samples are passed  through a
                         reduction column to reduce nitrate to
                         nitrite.   The nitrite undergoes a
                         diazotization reaction to form an azo
                         dye which is  determined spectrophoto-
                         metrically.

                         Granulated cadmium, phosphoric acid,
                         sulfanilamide,  N-(1-naphtyl)-ethylene-
                         diame dihydrochloride,  Brij-35, hydro-
                         chloric acid, copper sulfate, ammonium
                         chloride.

Overall Glove Recommendation:   Chlorinated Polyethylene (CPE)
 Chemicals Used:
SAFETY CONTROLS
Procedure
       Granulated
                      Potential Exposure

                       Inhalation
cadmium
2.  Color  reagent
preparation,
phosphoric   acid,
sulfanilamide,   N-
(1-naphtyl)-
ethylenediamine
dihydrochloride,
Brij-35
                       Spills,  splashing,
                       inhalation
     PPE and/or
Engineering Controls

  Do  procedure  in  a
  fume  hood.    PPE-
  splash proof safety
  goggles,  lab coat,
  rubber  gloves  (no
  data  on   gloves).
  CPE adequate

  Do  procedure  in  a
  fume  hood.   Avoid
  any high heating of
  this    material.
  Phosphoric    acid
  reacts  with  water
  and sulfanilamide.
  Mix    carefully.
  PPE-splash    proof
  safety goggles, lab
  coat,    viton,  or
  butyl,    or    CPE
  gloves

-------
  Procedure

  3.     Hydrochloric
  acid,  reagent water
  solution
 4.  Copper  sulfate
 solution
 5. Buffer  solution
 preparation,
 ammonium chloride,
 Brij-35
 6.    Standards
 handling


 7.  Procedure:

 7a.  Preparation of
 reduction   column,
 cadmium,
 hydrochloric acid
7b. Preparation of
reduction   column,
cadmium,     copper
sulfate
Potential Exposure

 Spills,  splashing,
 inhalation
 Spills, splashing,
 inhalation
 Spills,  splashing,
 inhalation
As  listed  in  SOP
No. 19
Spills, splashing,
inhalation
Spills,  splashing,
inhalation
                9-2
     PPE and/or
Engineering Controls

  May  cause  skin  or
  respiratory burns.
  Do  procedure in  a
  fume  hood.    PPE-
  splash proof safety
  goggles,  lab coat,
  neoprene,    rubber
  gloves    or   CPE
  gloves

 Avoid heating this
 material.       Do
 procedure in a fume
 hood.    PPE-splash
 proof     safety
 goggles, lab coat,
 neoprene,    nitrile
 or CPE gloves

 Do not  burn  this
 material.        Do
 procedure  in a fume
 hood.    PPE-splash
 proof    safety
 goggles,  lab coat,
 neoprene,  nitrile,
 butyl,   viton,   or
 CPE gloves

 As  listed  in  SOP
 No.  19.   Gloves  -
 CPE
Do  procedure in a
fume  hood.   PPE-
splash proof safety
goggles, lab coat,
neoprene, rubber or
CPE gloves

Avoid heating.   Do
procedure in a fume
hood.   PPE-splash
proof     safety
goggles, lab coat,
neoprene,   nitrile
or CPE gloves

-------
 Procedure

 7c.Preparation   of
 reduction   column/
 cadmium handling
 8.    Analytical
 system    handling
 standards

 9. Conditioning the
 pump/    ammonium
 chloride
 10.     Preliminary
 calibration/ dilute
 standards
Potential Exposure

 Inhalation
 As  listed  in  SOP
 No. 19
 Spills,  splashing,
 inhalation
As  listed  in  SOP
No. 19
                9-3
     PPE and/or
Engineering Controls

  Do  procedure in  a
  fume  hood.    PPE-
  splash proof safety
  goggles/  lab coat,
  rubber or neoprene
  gloves (no  data  on
  gloves).       CPE
  adequate

 As  listed  in SOP
 No. 19.   Gloves  -
 CPE

 Allow local  exhaust
 or    venting
 operating
 equipment.    PPE-
 splash proof safety
 goggles/  lab coat/
 neoprene/  nitrile/
 butyl,   viton/  or
 CPE gloves

 As   listed   in  SOP
 No.  19.   Gloves  -
 CPE
WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.

SPECIAL PRECAUTIONS

Follow Trouble Shooting / Corrective Action Section

Wash hands thoroughly after procedure completion.

-------
                                                              10-1
 Laboratory Procedure:

 Method:

 Description:
 Chemicals  Used:
 CHEMICAL HYGIENE SOP

        NO. 10

    Dissolved  Oxygen

    Micro Method, Winkler Titration

    The  sample is treated with several
    chemicals.  Manganous hydroxide combines
    with dissolved oxygen in the sample to
    form a brown precipitate.  Free iodine
    is released which is stoichiometrically
    equivalent to the dissolved oxygen in
    the  sample.  The sample is then titrated
    with sodium thiosulfate.

    Manganese sulfate, sodium hydroxide,
    sodium iodide, sodium azide,  sulfuric
    acid, soluble  starch, sodium thiosulfate,
    potassium biiodate,  potassium iodide,
   chloroform.
Overall Glove Recommendation:
         Chlorinated Polyethylene (CPE)
         except for chloroform,  see
         procedure 14
SAFETY CONTROLS
Procedure

1.    Manganese
sulfate    solution
preparation
Potential Exposure

 Inhalation
     PPE and/or
Engineering Controls

  General ventilation
  is  adequate.    Do
  procedure in a fume
  hood  if  practical.
  PPE-splash    proof
  safety goggles, lab
  coat,  no  data  on
  gloves,   recommend
  rubber    or    CPE
  gloves

-------
 Procedure

 2.  Alkaline azide
 reagent,    sodium
 hydroxide,  sodium
 iodide,    sodium
 azide
Potential Exposure

 Spills,  splashing/
 inhalation
3. Starch indicator
solution
None
4  .    Sodium
thiosulfate   stock
solution,
chloroform
Spills,  splashing,
inhalation
               10-2
     PPE and/or
Engineering Controls

  Sodium  azide   is
  extremely poisonous
  and    may    be
  explosive  at  very
  high heat.   Sodium
  hydroxide is highly
  toxic.         Both
  materials     react
  violently    with
  water.  Mix slowly,
  allow to cool.  Do
  procedure in a fume
  hood.   PPE-splash
 proof     safety
 goggles,     face
 shield,  lab coat,
 nitrile,  neoprene
 or  rubber  gloves
  (choice),  butyl or
 CPE    gloves
 (adequate).   Apron
 optional

 General ventilation
 adequate.      PPE-
 safety  glasses  or
 splash  proof,  lab
 coat,  any  type of
 protective    glove
 optional.       CPE
 suggested

 Chloroform   is   a
 suspect carcinogen.
 Do  procedure   in
 Designated     Area
 fume  hood.    PPE-
 splash proof safety
 goggles, lab coat,
 viton gloves only

-------
                                                              10-3
 Procedure

 5 .    S o d i u
 thiosulfat
 standard titrant
m
e
Potential Exposure

 None
 6.    Potassium
 biiodate    stock
 solution,   working
 standard
      Drying  (burns)
7. Potassium iodide
solution
preparation
     None
8.  Sulfuric  acid
solution
     Spills,  splashing,
     inhalation
     PPE and/or
Engineering Controls

  General ventilation
  is  adequate.    If
  practical,     do
  procedure  in  fume
  hood.    PPE-safety
  glasses  or  splash
  proof,   lab   coat,
  nitrile,  neoprene,
  viton, butyl or CPE
  gloves

  Use   local    heat
  exhaust     for
  heating.   General
  ventilation
  adequate     for
  preparation.    PPE-
  safety glasses, lab
 coat,   gloves   not
 required, vinyl  or
 CPE gloves optional

 General ventilation
 adequate.        If
 practical,     do
 procedure in a  fume
 hood.    PPE-safety
 glasses  or  splash
 proof,   lab   coat,
 any   protective
 glove     optional.
 CPE  suggested

 General ventilation
 is  adequate.    If
 practical,     do
 procedure in a fume
 hood.    PPE-splash
 proof    safety
 goggles,  lab coat,
 viton, chlorinated
 polyethylene,
 polyethylene gloves

-------
                                                              10-4
                                                   PPE and/or
 Procedure             Potential Exposure     Engineering Controls

 9.  Standardization     Spills, splashing,     Low concentrations,
 of    sodium     inhalation             general ventilation
 thiosulfate                                   adequate.      PPE-
                                               splash proof safety
                                               goggles/  lab coat,
                                               viton or CPE gloves

 10.  Procedure           Spills, splashing,     If  practical,   do
                        inhalation             procedures   in   a
                                               fume hood.  Use PPE
                                               as listed for  each
                                               reagent handled


WASTE GENERATION AND HANDLING

Follow  all  guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.


SPECIAL PRECAUTIONS

Mix alkaline azide solution carefully to avoid violent reaction.
Recommend double gloving with carcinogens  in the event of a spill.

Wash hands thoroughly after procedure completion.

-------
                                                              11-1

                        CHEMICAL HYGIENE SOP

                               No.  11

 Laboratory Procedure:    Dissolved Oxygen

 Method:                  Micro, dissolved oxygen meter measurement

 Description:             Samples are analyzed with a Dissolved
                          Oxygen meter immediately after collection.

 Chemicals Used:          Potassium chloride

 Overall Glove Recommendation:   Vinyl

 SAFETY CONTROLS

                                                   PPE  and/or
 Procedure             Potential Exposure     Engineering  Controls

 Addition potassium     None                   General ventilation
 chloride  solution                            adequate.      PPE-
 to  probe                                      safety glasses, lab
                                               coat,    any
                                               protective gloves.
                                               Vinyl suggested

WASTE  GENERATION AND  HANDLING

Follow all guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.


SPECIAL  PRECAUTIONS

Wash hands thoroughly after procedure completion.

-------
                                                             12-1
 Laboratory Procedure:

 Method:

 Description:




 Chemicals  Used:
 CHEMICAL HYGIENE SOP

        No.  12

   Electometric pH
   The pH of a sample is determined
   electrometrically using a glass electrode
   in combination with a reference electrode
   or with a combination pH electrode.

   Calibration standard buffers, control
   standard buffers,  sodium hydroxide  for
   cleaning.
Overall  Glove  Recommendation:  Chlorinated Polyethylene (CPE)

SAFETY CONTROLS
Procedure

1.    Calibration
using  pH   buffers
( potassium
biphthalate-sodium
hydroxide buffer)
2.   Washing  with
sodium hydroxide
Potential Exposure

 Spills,  splashing,
 inhalation
 Spills,  splashing,
 inhalation
     PPE and/or
Engineering Controls

  General ventilation
  may   be   adequate.
  PPE-splash    proof
  safety goggles, lab
  coat,  gloves   not
  reguired, neoprene,
  butyl,   rubber  or
  CPE gloves optional

  When   in   contact
  with  water,  sodium
  hydroxide    may
  generate sufficient
  heat   to    ignite
  combustibles.    Do
  procedure in a fume
  hood,    collect
  rinse-off.     PPE-
  splash proof safety
  goggles,  lab coat,
  nitrile,  neoprene,
  rubber    gloves
  (choice), butyl  or
  CPE     gloves
  (adeguate)

-------
                                                             12-2

WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.

SPECIAL PRECAUTIONS

Wash hands thoroughly after procedure completion.

-------
                                                              13-1
                        CHEMICAL HYGIENE SOP

                               No. 13

 Laboratory Procedure:    Soluble Reactive Phosphorous

 Method:                  	

 Description:             Phosphate ions react with ammonium
                          molybdate and potassium antimonyl
                          tartrate.  It is reduced with ascorbic
                          acid to form a blue complex which is
                          determined spectrophotometrically.

 Chemicals Used:          Sodium lauryl sulfate, sulfuric acid,
                          ammonium molybdate, ascorbic acid,
                          potassium antimonyl tartrate,  hydro-
                          chloric acid, sodium hydroxide.

 Overall Glove Recommendation:   Chlorinated Polyethylene (CPE)
 SAFETY CONTROLS
 Procedure

 1.   Sodium  lauryl
 sulfate    solution
 preparation
Potential Exposure

 Splashing
2.  Sulfuric   acid
solution
preparation
Spills,  splashing,
inhalation
     PPE and/or
Engineering Controls

 General ventilation
 adequate.        Do
 procedure  in  fume
 hood  if  practical.
 PPE-splash    proof
 safety goggles, lab
 coat, rubber gloves
 ( choice) ,    any
 protective    glove
 (adequate).     CPE
 suggested

 Do  procedure in  a
 fume  hood.    May
 cause   skin   and
 respiratory  burns.
 PPE-splash   proof
 safety goggles, lab
 coat,    viton,
 chlorinated
 polyethylene,
 polyethylene  gloves

-------
  Procedure

  3.    Ammonium
  molybdate    stock
  solution
 Potential Exposure

  Spills,  splashing,
  inhalation
  4.   Ascorbic  acid
  solution
 Slight irritations
 5.     Potassium
 antimonyl  tartrate
 solution
 Spills,  splashing,
 inhalation
 6.  Combined  color
 reagent mixture
 Spills,  splashing,
 inhalation
7.     Standards
preparation    and
handling
Follow SOP No.  16
                13-2
     PPE and/or
Engineering Controls

  General ventilation
  is  adequate.   PPE-
  splash proof safety
  goggles,  lab  coat,
  no  data  on gloves,
  recommend rubber or
  CPE gloves

  General ventilation
  is  adequate.     If
  practical,    do
  procedure  in   fume
  hood.   PPE-splash
  proof    safety
  goggles, lab  coat,
  any   protective
  glove.        CPE
  suggested

 General ventilation
 is adequate.  PPE-
 splash proof safety
 goggles,  lab  coat,
 no data on gloves-
 recommend rubber or
 CPE

 General ventilation
 is    adequate.
 Follow  analytical
 procedure   steps
 when    adding
 chemicals.      Add
 slowly,     mix
 carefully,   allow
 mixture to cool.
 PPE-splash   proof
 safety goggles, lab
coat,    use
appropriate  gloves
for    handling
sulfuric    acid,
remaining solutions
and handling of nix
- CPE adequate

Follow SOP No.   16.
Gloves - CPE

-------
 Procedure

 8.  Procedure
 9.    Hydrochloric
 acid preparation
Potential Exposure

 Follow SOP No.  19
 Spills,  splashing,
 inhalation
10.    Trouble
Shooting sequencing
hydrochloric acid
 Inhalation
11.    Sodium
hydroxide
preparation
Spills,  splashing,
inhalation
                13-3
     PPE and/or
Engineering Controls

  Follow SOP No.  19.
  Gloves -  CPE

  May   cause  severe
  skin or respiratory
  burns.        Do
  procedure in a fume
  hood.    PPE-splash
  proof    safety
  goggles/  lab  coat,
  neoprene, rubber or
  CPE gloves

 Acid   is   dilute,
 general ventilation
 adequate.     PPE-
 splash proof safety
 goggles, lab coat,
 neoprene, rubber or
 CPE gloves.   Seal
 container    with
 parafilm

 When   in   contact
 with water, sodium
 hydroxide    may
 generate sufficient
 heat   to   ignite
 combustibles.    Do
 procedure in a fume
 hood.    PPE-splash
 proof    safety
 goggles, lab coat,
 nitrile, neoprene,
 rubber    gloves
 (choice),  butyl or
 CPE    gloves
 (adequate)

-------
                                                              13-4
                                                    PPE and/or
 Procedure             Potential Exposure     Engineering Controls

 12.    Trouble     Splashing              Sodium    hydroxide
 Shooting sequencing                            soln.    is    quite
 with    sodium                            dilute.     General
 hydroxide                                     ventilation    is
                                               adequate.      PPE-
                                               splash proof safety
                                               goggles/ lab  coat,
                                               nitrile,  neoprene
                                               rubber    gloves
                                               (choice)/ butyl or
                                               CPE     gloves
                                               (adequate)


WASTE GENERATION AND HANDLING

Follow all  guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.


SPECIAL PRECAUTIONS

Mix combined color reagent very carefully.

Wash hands thoroughly after procedure completion.

-------
                                                             14-1
                        CHEMICAL HYGIENE  SOP

                               No.  14
                          Total phosphorous,  total dissolved
                          phosphorous

                          Low level,  micro-persulfate digestion,
                          automated spectrophotometric deter-
                          mination.

                          Water samples  are digested - blue
                          complex is  determined  spectrophoto-
                          metrically.

                          Sulfuric acid, hydrochloric acid,
                          ammonium persulfate, ammonium molybdate,
                          ascorbic acid, potassium  antimonyl
                          tartrate, sodium lauryl sulfate

 Overall  Glove  Recommendation:   Chlorinated Polyethylene  (CPE)

 SAFETY CONTROLS
Laboratory Procedure:
Method:
Description:
Chemicals Used:
Procedure

1  .    Sample
preservation   with
sulfuric acid
                      Potential Exposure

                       Spills, splashing,
                       inhalation
2.  Preparation of
hydrochloric acid
                       Spills,  splashing,
                       inhalation
     PPE and/or
Engineering Controls

  May cause  skin or
  respiratory burns.
  General ventilation
  adequate  for  this
  procedure  but  use
  caution.       PPE-
  splash proof safety
  goggles,  lab coat,
  viton,  chlorinated
  polyethylene ,
  polyethylene gloves

  May cause   severe
  skin or respiratory
                                              burns
                                                               Do
                                              procedure in a fume
                                              hood.    PPE-splash
                                              proof    safety
                                              goggles,  lab  coat,
                                              neoprene, rubber or
                                              CPE gloves

-------
 Procedure

 3.     Digestion
 solution
 preparation,
 sulfuric acid
 4.     Digestion
 solution
 preparation,
 ammonium persulfate
 5.    Working
 digestion solution,
 sulfuric     acid,
 ammonium persulfate
6.  Sampler  wash,
sulfuric acid
Potential Exposure

 Spills,  splashing,
 inhalation
 Spills, splashing,
 inhalation
 Spills,  splashing,
 inhalation,
 potential   violent
 reactions
Spills, splashing,
inhalation
                14-2
     PPE and/or
Engineering Controls

  May cause  skin  or
  respiratory burns.
  Do procedure  in  a
  fume  hood.    PPE-
  splash proof safety
  goggles,  lab coat,
  viton,  chlorinated
  polyethylene,
  polyethylene gloves

  This material  is  a
  strong    oxidizer
  when heated.   Keep
  from combustibles.
  do  procedure in  a
  fume  hood.     PPE-
  splash proof safety
 goggles, lab coat,
 neoprene,  viton or
 butyl or CPE  gloves

 Sulfuric  acid  is
 not compatible with
 strong   oxidizers.
 Mix    solution
 carefully,    avoid
 heating   and  heat
 sources.    General
 ventilation
 adequate at  10 ml
 volumes   of   each
 reagent.       PPE-
 splash proof safety
 goggles  and  face
 shield,  lab  coat,
viton or CPE gloves
only,  viton  apron
recommended

Very  dilute  acid.
General ventilation
is adequate.  PPE-
splash proof safety
goggles,  lab  coat,
viton, chlorinated
polyethylene,
polyethylene gloves

-------
 Procedure
 7  .     Color
 development
 reagent,  sulfuric
 acid
Potential Exposure
 Spills, splashing,
 inhalation
 8.    Ammonium
 molybdate    stock
 solution
 preparation
9.   Ascorbic  acid
stock    solution
preparation
 Spills,  splashing,
 inhalation
Slight irritations
10.     Potassium
antimonyl tartrate
solution
Spills, splashing,
inhalation
                14-3
     PPE and/or
Engineering Controls
  May  cause skin  or
  respiratory  burns.
  Do  procedure in  a
  fume  hood.    PPE-
  splash proof safety
  goggles,  lab  coat,
  viton,  chlorinated
  polyethylene,
  polyethylene gloves

  General ventilation
  adequate.     PPE-
  splash proof safety
  goggles, lab coat,
  no data on gloves -
  recommend rubber or
 CPE

 General  ventilation
 adequate.       If
 practical,     do
 procedure in a fume
 hood.    PPE-splash
 pr-oof    safety
 goggles,  lab coat,
 any    protective
 glove.        CPE
 suggested

 General ventilation
 adequate.      PPE-
 splash proof safety
 goggles,  lab  coat,
 no data on gloves  -
 recommend rubber or
 CPE

-------
                                                               14-4
  Procedure

  11.  Combined color
  reagent mixture
Potential Exposure

 Spills,  splashing,
 inhalation
 12.   Sodium lauryl
 sulfate    solution
 preparation
 Splashing
 13.    Standard
 preparation

 14.     Digestion
 sample, calibration
and    controls
preparation
Follow SOP No. 16
Spills, splashing,
inhalation
     PPE and/or
Engineering Controls

  General ventilation
  and good  lab prac-
  tice is  adequate.
  Follow   analytical
  procedure    steps
  when    adding
  chemicals.      Add
  slowly,     mix
  carefully,    allow
  mixture   to   cool.
  PPE-splash    proof
  safety goggles, lab
  coat,  use approp-
  riate  gloves   for
  handling  sulfuric
  acid,    remaining
  solutions  and
 handling of mix-CPE
 gloves

 General ventilation
 is  adequate.    If
 practical,     do
 procedure  in a fume
 hood.    PPE-splash
 proof    safety
 goggles, lab  coat,
 rubber    gloves
 (choice),     any
 protective   glove
 (adequate)
Follow SOP No.
Gloves - CPE
16.
General ventilation
is adequate.  Wear
appropriate PPE as
outlined     for
reagents     and
controls

-------
                                                              14-5
                                                   PPE  and/or
 Procedure             Potential Exposure     Engineering Controls


 15.    Digestion/     Burns, inhalation      Use  exhaust   hood
 heating tubes                                 while   digesting.
                                               Allow a clean air
                                               purge     before
                                               opening.  Use
                                               insulated   gloves
                                               when handling hot
                                               items

 16.    Analytical     Inhalation             Follow   procedures
 procedures                                     and PPE  in SOP No.
                                               19.   Gloves -  CPE

 WASTE  GENERATION AND HANDLING

 Follow all guidelines in the U.S. EPA's Characterization of Waste
 From Standard Analytical Methods.

 SPECIAL PRECAUTIONS

Combined color  reagent  mix should be done carefully/  as  well as
working digestion solutions.  Avoid breathing digestion  vapors off
of heating apparatus.

Wash hands thoroughly after procedure completion,.

-------
                                                              15-1
 Laboratory Procedure:

 Method:


 Description:
                       CHEMICAL HYGIENE  SOP

                              No.  15

                         Silicates

                         Automated spectrophotometric,  molybdate
                         blue

                         In acidic solution/  silicamolybdate  is
                         reduced to molybdenum blue  which  is
                         determined spectrophotometrically
                         Ammonium molybdate, sulfuric acid,
                         oxalic acid, ascorbic acid, acetone
Chemicals Used:


Overall Glove Recommendation:   Chlorinated Polyethylene  (CPE)
SAFETY  CONTROLS
Procedure

1.     Ammonium
molybdate  reagent,
ammonium molybdate,
sulfuric acid
                      Potential Exposure

                       Spills,  splashing,
                       inhalation
2.   Oxalic
reagent
              acid
Spills,  splashing,
inhalation
     PPE and/or
Engineering Controls

  Do  procedure in  a
  fume  hood.    PPE-
  splash proof safety
  goggles,  lab coat,
  viton or CPE gloves

  Incompatible  with
  oxidizers    and
  sulfuric    acid.
 Avoid this contact.
 Avoid     heating.
 General ventilation
 is adequate.  PPE-
 splash proof safety
 goggles, lab coat,
 rubber,     butyl,
 chlorinated
 polyethylene gloves
 (choice),  nitrile,
 CPE   or   neoprene
 gloves  (adequate)

-------
 Procedure

 3.  Ascorbic  acid
 reagent,  ascorbic
 acid, acetone
 4.    Standards
 solution

 5.    Analytical
 Procedures
Potential Exposure

 Splashing ,
 inhalation ,
 flammable
Follow SOP No. 16
Follow SOP No. 19
                15-2
     PPE and/or
Engineering Controls

  Acetone    is
  flammable.    Keep
  from    ignition
  sources.         Do
  procedure  in a fume
  hood.     Remaining
  procedures can  be
  done   in   general
  ventilation.   PPE-
  splash proof safety
  goggles, lab  coat,
  butyl or CPE gloves
  only.  Seal reagent
  top with parafilm

  Follow SOP No. 16
 Follow SOP No. 19
WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.
SPECIAL PRECAUTIONS

Use caution working with flammables.

Wash hands thoroughly after procedure completion

-------
                                                             16-1
 Laboratory Procedure:

 Method:

 Description:


 Chemicals Used:
 CHEMICAL HYGIENE SOP

        No. 16

   Standards and Spikes Preparation

   Many

   Preparation of individual standards
   used for the analytical procedures.

   Sulfuric acid, chloroform/ anhydrous
   ammonium sulfate, potassium biphthalate,
   sodium chloride,  potassium nitrate,
   potassium nitrite, sodium nitrite,
   potassium phosphate (monobasic),  silica,
   sodium sulfate,  ammonium chloride,
   methenamine,  potassium chloride,
   potassium sulfate, sodium nitrate,
   sodium phosphate  (dibasic),  sodium
   glycerophosphate,  sodium silicate
Overall Glove Recommendation:

SAFETY CONTROLS
         Chlorinated Polyethylene  (CPE)
Procedure
Calibration   (set)
standards  ,
preparation:

1.  Ammonia   stock
standard,  anhydrous
ammonium  sulfate,
sulfuric acid
Potential Exposure
 Splash,    possible
 explosion
     PPE and/or
Engineering Controls
 Avoid  extreme heat
 w/ ammonium sulfate
 or  sulfuric  acid-
 explosive.   Do not
 mix    ammo n i urn
 sulfate     with
 oxidizers-produces
 toxic gases.   When
 adding    sulfuric
 acid, do procedure
 in fume hood.   All
 other dilutions of
 the stock standard
 can  be   done  in
 general
 ventilation.  Do in
 fume    hood    if
 practical

-------
 Procedure
Potential Exposure
 2.     Dissolved
 organic    carbon,
 potassium
 biphthalate    and
 sulfuric acid
 3.   Chloride  stock
 standard,    sodium
 chloride
 Spills, splashing,
 inhalation
 None
4.   Nitrate  stock
standard, potassium
nitrate,   sulfuric
acid
Spills,  splashing,
inhalation
5.   Nitrite   stock
standard, potassium
nitrite,     sodium
nitrite, chloroform
Spills,  splashing,
inhalation
6.    Phosphorous
stock    standard,
potassium phosphate
(monobasic),
sulfuric acid
Spills, splashing,
inhalation
               16-2
     PPE and/or
Engineering Controls

  PPE-splash   proof
  safety goggles, lab
  coat,     viton,
  chlorinated
  polyethylene,
  polyethylene gloves

  Low  cone,  of  acid
  general ventilation
  is adequate.   PPE-
  splash proof safety
  goggles, lab coat,
 viton, chlorinated
 polyethylene,
 polyethylene gloves

 General ventilation
 adequate.   General
 lab  PPE  required:
 Safety glasses, lab
 coat,     any
 protective    glove
 optional.       CPE
 suggested

 Do procedure  in  a
 fume   hood.    PPE-
 splash proof safety
 goggles, lab coat,
 viton,  chlorinated
 polyethylene,
 polyethylene gloves

 Chloroform   is   a
 suspect carcinogen.
 Do procedure in the
 Designated     Area
 fume  hood.    PPE-
 splash proof safety
 goggles, lab coat,
 viton or CPE gloves

 Do  procedure in  a
 fume  hood.     PPE-
 splash proof safety
 goggles, lab  coat,
 viton, chlorinated
 polyethylene,
 polyethylene gloves

-------
  Procedure

  7.    Silica
  standard
stock
Potential Exposure

 Inhalation
  8.   Sulfate  stock
  standard,    sodium
  sulfate
          Slight inhalation
 Intermediate
 control  standards,
 working     control
 standards
 (dilutions     of
 intermediate) ,
 spike preparations

 9.  Ammonia  /
 orthorphosphate,
 sulfuric acid
         Spills, splashing,
         inhalation
 10.  Nitrate/silica
 intermediate
11.     Digested
phosphorous ,
sulfuric acid
         Spills,  splashing,
         inhalation
        Spills,  splashing,
        inhalation
                16-3
     PPE and/or
Engineering Controls

  Do procedure in  a
  fume  hood.    PPE-
  safety glasses, lab
  coat,     any
  protective    glove
  optional.       CPE
  suggested

  General ventilation
  is adequate.  PPE-
  splash proof safety
  goggles, lab  coat,
  any    protective
 glove.        CPE
 suggested
                       General ventilation
                       may  be  adequate.
                       If  practical,  do
                       procedure in a fume
                       hood.   PPE-splash
                       proof     safety
                       goggles, lab coat,
                       viton,  chlorinated
                       polyethylene,
                       polyethylene gloves

                       General ventilation
                       is adequate.   PPE-
                       splash proof safety
                       goggles,  lab coat,
                       viton or CPE gloves

                       Do procedure in a
                       fume  hood.    PPE-
                       splash proof safety
                       goggles, lab  coat,
                       viton, chlorinated
                       polyethylene,
                       polyethylene gloves

-------
 Procedure

 12.   Chloride
 sulfate
               Potential Exposure

                None
 Control   standard
 concentrate    for
 autoanalyzers,
 preparation:
 1 3
Ammon ium
Inhalation
 chloride
 14.    Dis solved
 organic    carbon,
 methenamine
               Spills, splashing,
               inhalation ,
               flammable
15.  Chloride   and
sulfate  stock  and
high & low control
concentrate ,
potassium chloride,
potassium sulfate
               None
                                      16-4
                           PPE and/or
                      Engineering Controls

                       General ventilation
                       is    adequate.
                       General   lab   PPE-
                       safety glasses, lab
                       coat,     any
                       protective  glove.
                       CPE suggested
 General ventilation
 is  adequate,  low
 cone.    PPE-splash
 proof    safety
 goggles,  lab coat,
 chlorinated
 polyethylene
 gloves.        Seal
 ammonium   chloride
 buffer    with
 parafilm

 General ventilation
 is   adequate,   low
 cone.    Keep  from
 ignition   sources.
 PPE-splash    proof
 safety goggles, lab
 coat,  (no data  on
 gloves),  recommend
 rubber  gloves   or
 CPE   and    double
 gloving

 General ventilation
 adequate.         If
 practical,    do
 procedure  in  fume
 hood.   PPE-safety
glasses, lab coat,
any    protective
glove.  Suggest CPE

-------
 t ^.ocedure
 16.  Nitrate stock
 control,    sodium
 nitrate
Potential Exposure
 Spills, inhalation
 17. Orthophosphate
 stock    control,
 sodium   phosphate
 (dibasic)
 18.    Digested
 phosphorous, sodium
    cerophosphate
 Slight    spills,
 inhalation
 19.   Silica   stock
 control, Naj Si F,
Intermediate
control  standards,
working    control
standards  ,
preparation:

20. Ammonia /
orthophosphate,
sulfuric acid (high
&  low standard)
Inhalation, spills
Spills, splashing,
inhalation
                16-5
     PPE and/or
Engineering Controls
  Avoid  any shock or
  heating   to   this
  material.   General
  ventilation    is
  adequate.      This
  material is a solid
  and may disperse in
  the    fume    hood.
  PPE-splash    proof
  safety goggles, lab
  coat, butyl, CPE or
  viton gloves

  General ventilation
  is adequate.   PPE-
  safety glasses, lab
 coat,    any
 protective  glove.
 CPE suggested

 General ventilation
 is adequate.   PPE-
 safety  glasses, lab
 coat,    any
 protective  glove.
 CPE suggested

 Local   ventilation
 adequate.     PPE-
 safety glasses, lab
 coat,  any  protec-
 tive  glove.    CPE
 suggested
General ventilation
is     adequate.
Quantity  of  acid
used is low.  PPE-
splash proof safety
goggles, lab coat,
viton, chlorinated
polyethylene,
polyethylene gloves

-------
                                                              17-1
                        CHEMICAL HYGIENE SOP

                               No.  17
 Laboratory Procedure:    Sulfate
 Method:
                         Automated, methylthymol blue, spectro-
                         photometric determination

                         Water samples are passed through various
                         chemical reactions to determine the
                         amount of sulfate present

                         Barium chloride,  methylthymol blue,
                         hydrochloric acid,  ethanol,  ammonium
                         chloride, ammonium hydroxide,  tetra-
                         sodium EDTA, sodium hydroxide,  Brij-
                         35,  sodium sulfate,  calcium  oxide

Overall Glove Recommendation:   Chlorinated Polyethylene  (CPE)
 Description:
 Chemicals Used:
 SAFETY CONTROLS

 Procedure
v'l.  Barium chloride
 solution
 preparation
                      Potential Exposure
 2.    Methylthymol
 blue    solution
 preparation
                       Spills,  splashing,
                       inhalation
     PPE and/or
Engineering Controls
  Perform  procedure
  in  fume hood.  PPE-
  splash proof safety
  goggles,  lab coat,
  any    protective
  glove.        CPE
  suggested

  Do   procedure   in
  fume hood.  Ethanol
  vapors  may   flash
  back.    Keep  from
  open  flame.    PPE-
  splash proof safety
  goggles, lab coat,
  polyethylene gloves
  only  for  ethanol,
  rubber  (choice)  or
  viton    or    CPE
  (adequate) gloves

-------
  Procedure

  3.  Buffer  solution
  preparation
Potential Exposure

 Spills,  splashing,
 inhalation
 4.  Buffered   EDTA
 solution
 preparation
 5.  Sodium hydroxide
 solution
 preparation
 Spills, splashing,
 inhalation
 Spills,  splashing,
 inhalation
6.  Dilution water
preparation
Spills,  splashing,
inhalation
                17-2
     PPE and/or
Engineering Controls

  General ventilation
  is adequate.   PPE-
  splash proof safety
  goggles,  lab coat,
  neoprene,   CPE   or
  rubber    gloves.
  Seal   reagent  and
  waste   container
  with  parafilm

  General ventilation
  is  adequate.  PPE-
  splash proof safety
  goggles, lab  coat,
  neoprene,  CPE  or
  butyl gloves

 When   in   contact
 with water, sodium
 hydroxide   may
 generate sufficient
 heat   to   ignite
 combustible
 materials.     Keep
 these   materials
 away.       Prepare
 solution  in a fume
 hood.    PPE-splash
 proof    safety
 goggles, lab coat,
 nitrile,  neoprene,
 rubber    gloves
 (choice), butyl or
CPE    gloves
 (adequate)

General ventilation
is  adequate.   If
practical,    do
procedure  in  fume
hood.   PPE-splash
proof   safety
goggles, lab coat,
rubber    gloves
(choice),     any
protective   glove
(adequate).     CPE
suggested

-------
 Procedure

 7.   Sulfate  stock
 solution
 preparation ,
 working   solution
 dilutions

 8.     Analytical
 procedure

 9.     Pump     all
 reagents   through
 system,  run pattern
 for    calibration
 standards, handling
 samples,  analysis
 10.     Wash
 procedure
out
        Potential Exposure

        Follow SOP No. 16
        Follow SOP No. 19
        Spills, splashing/
        inhalation
Spills,  splashing,
inhalation
                                      17-3
                           PPE and/or
                      Engineering Controls

                       Follow SOP No.  16.
                       Gloves -  CPE
                       Follow SOP  No.  19.
                       Gloves - CPE

                       Use appropriate PPE
                       as indicated
Use appropriate PPE
as indicated
WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.

SPECIAL PRECAUTIONS

Use care in handling ammonium hydroxide.  Review Safety and Waste
Handling and Troubleshooting / Corrective Action  sections  in the
Laboratory Procedure Manual for this procedure.
Wash hands thoroughly after procedure completion.

-------
                                                              18-1
                        CHEMICAL HYGIENE SOP

                              No.  18

 Laboratory Procedure:    Suspended Solids

 Method:
 Description:



 Chemicals Used:

 SAFETY CONTROLS


 Procedure

 Drying in oven  or
 muffle furnace
   Glass fiber filters

   Water samples are filtered through a
   glass fiber filter.  This is washed,
   dried,  and weighed

   None
Potential Exposure

 Heat (burns)
     PPE and/or
Engineering Controls

  Exhaust  heat  from
  furnace.        Use
  insulated    gloves
  handling hot  items
WASTE GENERATION AND HANDLING

None.

SPECIAL PRECAUTIONS
None

-------
                                                             19-1
                        CHEMICAL HYGIENE  SOP

                              No.  19
 Laboratory Procedure;


 Method:

 Description:



 Chemicals Used:


 SAFETY CONTROLS


 Procedure

 Electrical
 connections,   wet
 conditions
   Technicon autoanalyzer and associated
   recording equipment
   This is a general description of
   operation of autoanalyzers,  recorders,
   and the computer connections.

   As listed in each SOP which  requires
   this equipment.
Potential Exposure

 Electrical  shock,
 hazards
     PPE and/or
Engineering Controls

 Use   Ground  Fault
 Interrupter
WASTE GENERATION AND HANDLING

None.
SPECIAL PRECAUTIONS

None

-------
                                                              20-1

                        CHEMICAL HYGIENE SOP

                               No. 20

                           Turbidity

                           Nephelometric

                           A comparison of light scattered by the
                           sample under defined conditions with the
                           intensity of light scattered by a standard
                           reference suspension.  Readings are made
                           in a nephelometer.

 Chemicals Used:           Hydrazine sulfate, hexamethylenetetramine

 Overall Glove Recommendation:  Polyvinyl chloride (Vinyl)
Laboratory Procedure:

Method:

Description:
 SAFETY CONTROLS
 Procedure
 1.   Stock formazin
 solution
 preparation,
 hydrazine sulfate,
 hexamethylenetetra
 mine
 2.    General
 procedure
                      Potential Exposure
                       Spills,  splashing,
                       inhalation
                      Inhalation, spills
     PPE and/or
Engineering Controls
  Respiratory    and
  skin   burns.     Do
  procedure in a fume
  hood.    PPE-splash
  proof    safety
  goggles, lab  coat,
  and    polyvinyl
  chloride gloves

  General ventilation
  is adequate.   PPE-
  safety  glasses   &
  lab coat.   Rubber
  or  vinyl   gloves
  optional
WASTE GENERATION AND HANDLING
Follow all guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.

SPECIAL PRECAUTIONS
Wash hands thoroughly after procedure completion.

-------
                                                              21-1
                        CHEMICAL HYGIENE  SOP
 Laboratory Procedure:



 Method:

 Description:
        No.  21

   Aerobic Heterotrophs.  (Total Coliforms
   Fecal Coliforms, Fecal Streptococci,
   not presently done).
   Microbiological analysis of water
   samples
 SOP for Aerobic Heterotrophs
 Chemicals Used:
   Sodium thiosulfate,  ethyl  alcohol,
   phosphate buffer solution
 Overall Glove Recommendation:  Chlorinated Polyethylene except for
                               hot items  (CPE).
 SAFETY CONTROLS
 Procedure
 1.    Addition   of
 sodium thiosulfate
 to    each   sample
 bottle
Potential Exposure
 None
2.   Heating   agar,
autoclaving
3. Flaming of flask
lip
 Heat  (burns)
Flammable
4.     Forceps
sterilized in ethyl
alcohol
Flammable
     PPE and/or
Engineering Controls
 General ventilation
 is  adequate.    If
 practical,     do
 procedure in a fume
 hood.    Suggested
 PPE-splash    proof
 safety goggles, lab
 coat,     nitrile,
 neoprene,    viton,
 CPE or butyl  gloves

 Exhaust heat.   Use
 insulated    gloves
 handling hot items

 Keep   combustible
 chemicals    and
 materials away from
 area.         If
 practical,   remove
 these from lab

 Keep container of
 ethyl alcohol 2 ft.
 from flame.  Vapors
 can    potentially
 flash back

-------
  Procedure

  5.     Analytical
  procedures ,
  handling
Potential Exposure

 Biological
 6. Sterilization of
 equipment
 Heat,  inhalation
 7. Calibration, pH
 buffers
Splashing
inhalation
WASTE  GENERATION AND HANDLING
                21-2
     PPE and/or
Engineering Controls

  General  bio-safety
  rules  should apply
  for all procedures.
  PPE-safety glasses,
  lab  coat,   gloves
  not  required,  any
  protective    glove
  optional.       CPE
  suggested

  Use exhausting vent
  or    hood    over
  autoclave.      Use
  insulated    gloves
 when handling  hot
 items

 General ventilation
 is adequate.   If
 practical,    do
 procedure in a fume
 hood.    PPE-splash
 proof    safety
 goggles,  lab coat,
 neoprene,    butyl,
 CPE    or    rubber
 gloves
Follow  all  guidelines  in  the U.S. EPA's Characterization of Waste
From  Standard  Analytical  Methods.


SPECIAL PRECAUTIONS

Follow  Biosafety Rules as outlined in the Chemical Hygiene Safety
Manual.  Recommend double gloving in the event of spill.

Wash hands thoroughly after procedure completion.

-------
 SOP for Fecal Coliform Bacteria
                                                             21-3
 Chemicals Used:
   Rosalie acid,  sodium hydroxide,
   potassium dihydrogen phosphate,  mag-
   nesium chloride
 Overall Glove Recommendation:
        Chlorinated Polyethylene except  for
         hot  items  (CPE).
 SAFETY CONTROLS
 Procedure
 1. Difco M-FC Broth
 preparation
Potential Exposure
 Spills,  splashing
2.   Rosalie   acid
solution
preparation
3.  Lauryl  Tryptos
Broth preparation
 Spills,  splashing,
 inhalation
Spills,  splashing,
inhalation,   heat
(burns)
     PPE and/or
Engineering Controls
  General ventilation
  adequate.    PPE-
  splash proof safety
  goggles,  lab coat,
  any   protective
  glove.        CPE
  suggested

  Perform   procedure
  in  fume hood. PPE-
  splash proof safety
  goggles, lab coat,
  rubber    or   CPE
  gloves

 General ventilation
 is adequate.  PPE-
 splash proof safety
 goggles,  lab coat,
 neoprene,   CPE  or
 rubber gloves.  Use
 care    during
 autoclaving    to
 prevent burns.  Use
 exhaust hood while
 autoclaving    and
 during removal  of
 products.       Use
 insulated    gloves
 for  hot items

-------
 Procedure

 4.    EC    medium
 preparation
Potential Exposure

 Spills, splashing,
 inhalation,   heat
 (burns)
 5.  Stock phosphate
 buffer    solution
 preparation,    pH
 adjustment
 Spills,  splashing,
 inhalation,    heat
 (burns)
6. Working solution
of phosphate buffer
Spills,  splashing,
inhalation,    heat
(burns)
                21-4
     PPE and/or
Engineering Controls

  General ventilation
  is  adequate.   PPE-
  splash proof safety
  goggles,  lab  coat,
  neoprene,   CPE   or
  rubber gloves.  Use
  care    during
  autoclaving     to
  prevent burns.  Use
  exhaust hood  while
  autoclaving    and
  during  removal   of
  products.   Use
  insulated   gloves
  for hot items

 General ventilation
 is adequate.  PPE-
 splash proof safety
 goggles,  lab coat,
 neoprene,   CPE  or
 rubber gloves.  Use
 care    during
 autoclaving    to
 prevent burns.  Use
 exhaust  hood while
 autoclaving    to
 prevent burns.  Use
 exhaust  hood while
 autoclaving    and
 during  removal  of
 products.       Use
 insulated    gloves
 for hot items

 General ventilation
 is adequate.   PPE-
 splash proof safety
 goggles, lab coat,
 neoprene,  CPE   or
rubber gloves.  Use
care    during
autoclaving.   Use
exhaust hood while
autoclaving    and
during removal of
products.       Use
insulated    gloves
for hot items

-------
Procedure

7  .    Sample
preparation     and
serial dilutions
Potential Exposure

 Spills,  splashing/
 inhalation,
 biological
8.    Filtration
procedure
 Spills,  splashing,
 inhalation ,
 biological
                21-5
     PPE and/or
Engineering Controls

  Biosafety    rules
  need    to    be
  followed  .
  Procedure  must be
  done    in    the
  Biological  Safety
  Cabinet.       PPE-
  splash proof safety
  goggles, lab  coat,
  CPE    or   rubber
  gloves    (double
  gloving   is
  recommended)

 Biosafety    rules
 need    to   be
 followed  .
 Procedure must  be
 done     in     the
 Biological  Safety
 Cabinet.       PPE-
 splash proof safety
 goggles,  lab coat,
 CPE    or    rubber
 gloves    (double
 gloving    is
 recommended).   Use
 care    while
 operating incubator
 to  prevent  burns.
 Use  exhaust  hood
 for     incubator
 operation    and
 removing   samples.
 Allow  a clean  air
 purge    before
 opening  incubator.
 Use     insulated
 gloves    for    hot
 items

-------
  Procedure

  9.     Verification
  procedures counting
  and  recording
Potential Exposure

 Spills, splashing,
 inhalation ,
 biological
                21-6
     PPE and/or
Engineering Controls

  Biosafety    rules
  need    to    be
  followed  .
  Procedure  must be
  done    in    the
  Biological   Safety
  Cabinet.       PPE-
  splash proof safety
  goggles, lab  coat,
  CPE    or    rubber
  gloves    (double
  gloving    is
 recommended).  Use
 care    while
 operating incubator
 to prevent  burns.
 Use exhaust  hood or
 vent    while
 incubator   is   in
 operation and while
 removing  samples.
 Allow a  clean  air
 purge    before
 opening incubator.
 General lab  rules
 and PPE.  Use
 insulated    gloves
 for hot items
WASTE  GENERATION AND HANDLING

Follow all  guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.
SPECIAL PRECAUTIONS

Biological  Safety procedures  need to be  followed during  these
procedures.  Thorough decontamination of work areas and equipment
needs to be followed closely.   All  biological work must be done in
a Biological  Safety  Cabinet.   Double gloving is  recommended for
protection in the event of a spill of inoculant.

Review all  Biosafety procedures in  the  Chemical Hygiene  Safety
Manual prior to any work.

-------
                                                             21-7
 SOP for  Total Coliform Bacteria

 Chemicals Used:          Ethanol, dipotassium hydrogen phosphate,
                         potassium dihydrogen phosphate, sodium
                         chloride, sodium lauryl sulfate, magnesium
                         chloride, methylene blue, sodium hydroxide

 Overall  Glove Recommendation:  Chlorinated Polyethylene (CPE)

 SAFETY CONTROLS
Procedure

1. M-Endo Broth
preparation
MF
Potential Exposure

 Spills,  splashing,
 inhalation,    heat
 (burns)
2.  To rehydrate M-
Endo Broth MF
       Spills,  splashing,
       inhalation ,
       flammables .
       Ethanol   is   very
       flammable
     PPE and/or
Engineering Controls

  General ventilation
  is  adequate.   PPE-
  splash proof safety
  goggles,  lab  coat,
  neoprene,   CPE  or
  rubber gloves.  Use
  exhaust hood during
  autoclaving.    Use
  insulated   gloves
  for heated material

  Ethanol   is   very
  flammable.       Do
  procedures   in   a
  fume  hood.    Keep
  from  open  flames.
 Keep    container
 closed. PPE-splash
 proof     safety
 goggles,  lab coat,
 polyethylene gloves
 only  for  handling
 ethanol,  rubber  or
 neoprene     for
 remaining
 procedure.  Boil  in
 a fume  hood.     Do
 not  use open flame
 heat source.   CPE
 gloves adequate for
 procedure

-------
 Procedure

 3.  Lauryl Tryptose
 Broth preparation
Potential Exposure

 Spills,  splashing,
 inhalation/    heat
 (burns)
 4.  Rehydration  of
 Lauryl    Tryptose
 Broth  preparation
Spills,  splashing,
inhalation,   heat
(burns)
5. Stock phosphate
buffer    solution
preparation,    pH
adjustment
Spills,  splashing,
inhalation,    heat
(burns)
                21-8
     PPE and/or
Engineering Controls

  General ventilation
  is  adequate.   PPE-
  splash proof safety
  goggles,  lab  coat,
  neoprene,   CPE   or
  rubber gloves.  Use
  care    during
  autoclaving     to
  prevent burns.  Use
  exhaust hood  while
  autoclaving    and
  during  removal   of
  products.        Use
  insulated   gloves
  for hot items

 General ventilation
 is adequate.  PPE-
 splash proof safety
 goggles,  lab coat,
 neoprene,   CPE  or
 rubber gloves.  Use
 care    during
 autoclaving    to
 prevent burns.  Use
 exhaust  hood while
 autoclaving    and
 during removal  of
 products.       Use
 insulated    gloves
 for  hot items

 General ventilation
 is adequate.   PPE-
 splash proof safety
 goggles, lab coat,
 CPE,   neoprene  or
 rubber gloves.  Use
 care    during
 autoclaving    to
 prevent burns.  Use
 exhaust hood while
 autoclaving    and
 during  removal  of
 products.      use
 insulated    gloves
 for hot items

-------
 Procedure

 6. Working solution
 of phosphate buffer
Potential Exposure

 Spills,  splashing,
 inhalation,    heat
 (burns)
7.  Brilliat  Green
Bile 2% preparation
Spills,  splashing,
inhalation,   heat
(burns)
                21-9
     PPE and/or
Engineering Controls

  General ventilation
  is  adequate.   PPE-
  splash proof safety
  goggles,  lab  coat,
  neoprene,   CPE  or
  rubber gloves.  Use
  care    during
  autoclaving     to
  prevent burns.  Use
  exhaust hood  while
  autoclaving    and
  during  removal  of
  products.       Use
  insulated   gloves
  for hot items

 General ventilation
 is adequate.   PPE-
 splash proof safety
 goggles, lab coat,
 neoprene,   CPE  or
 rubber gloves.  Use
 care    during
 autoclaving    to
 prevent burns.  Use
 exhaust hood while
 autoclaving    to
 prevent burns.  Use
 exhaust hood while
 autoclaving    and
 during removal  of
 products.       Use
 insulated    gloves
 for  hot items

-------
 Procedure

 8. Serial dilutions
Potential Exposure

 Spills, splashing,
 inhalation,
 biological
9.   Single   step
procedure
Spills, splashing,
inhalation,   heat
(burns), biological
               21-10
     PPE and/or
Engineering Controls

  Biosafety
  procedures must be
  followed .
  Procedure must  be
  done     in    the
  Biological   Safety
  Cabinet.       PPE-
  splash proof safety
  goggles,  lab  coat,
  CPE    or    rubber
  gloves.      Double
  gloving     is
  recommended .
 Biosafety
 procedures must be
 followed

 Procedure  must  be
 done    in    the
 Biological  Safety
 Cabinet.       PPE-
 splash proof safety
goggles, lab  coat,
CPE    or   rubber
gloves.     Double
gloving    is
recommended.
Biosafety
procedures must be
followed

-------
Procedure

10.   Single
procedure
                                FxDOSure
              step
Spills,  splashing,
inhalation,    heat
(burns), biological
1 1 .     Two-step
enrichment
procedure
                        Spills,  splashing,
                        inhalation,    heat
                        (burns), biological
               21-11
     PPE and/or
Engineering Controls

  Procedure must  be
  done     in    the
  Biological  Safety
  Cabinet.       PPE-
  splash proof safety
  goggles,  lab coat,
  CPE   or   rubber
  gloves,    double
  gloving    is
  recommended.    Use
  care    while
  operating incubator
  to  prevent  burns.
  Use  exhaust  hood
  while incubator is
  in  operation  and
  while     removing
  samples.    Allow  a
  clean  air   purge
  before    opening
  incubator.     Use
  insulated   gloves
  for hot items

  Procedure  must  be
  done    in    the
  Biological  Safety
  Cabinet.       PPE-
  splash proof safety
  goggles,   lab coat,
  CPE    or   rubber
  gloves,     double
  gloving     is
  recommended.   Use
  care    while
  operating incubator
  to  prevent burns.
  Use  exhaust  hood
  while incubator  is
  in  operation   and
  while    removing
  samples.   Allow  a
  clean   air  purge
  before    opening
  incubator.      Use
  insulated  gloves
  for hot  items

-------
                                                             21-12
                                                   PPE and/or
 Procedures             Potential Exposure     Engineering Controls

 12.  Counting  and     None                    General  lab  rules
 recording                                      and  PPE


 WASTE GENERATION AND HANDLING

 Thoroughly dilute and neutralize all materials and pour down drain.
 Follow all guidelines in  the  U.S.  EPA's Characterization of  Waste
 From Standard Analytical  Methods.
SPECIAL  PRECAUTIONS

Biological  safety practices  need to  be followed  during sample
procedures.  Thorough decontamination of work areas and equipment
needs to be followed closely.   All biological work must be done in
a  Biological  Safety  Cabinet.   Double gloving  is  recommended for
protection in the event of a spill of inoculant.

Review all  Biosafety Procedures  in  the Chemical Hygiene Safety
Manual prior to any work.

-------
                                                              21-13
  SOP for Fecal Streptococci
  Chemicals Used:
   Hydrogen peroxide, 2, 3, 5-Triphenyl
   tetrazolium chloride, sodium carbonate,
   bile salts
  Overall Glove Recommendation:   Chlorinated Polyethylene  (CPE)

  SAFETY CONTROLS
  Procedure

  1 .     BHI    agar
  preparation    KF
  streptococcus agar
 2.  Addition  of  2,
 3,    5-triphenyl
 tetrazolium
 chloride to agar

 3.    Analytical
 procedures,
 handling
 4.  Addition  of  3%
 hydrogen  peroxide
Potential Exposure

 Heat,  inhalation
Biological
None
WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S.
From Standard Analytical Methods,
     PPE and/or
Engineering Controls

 Exhaust  heat  while
 boiling.        Use
 insulated   gloves
 when  handling  hot
 items

 Missing MSDS
 General  biosafety
 rules should apply
 for all procedures.
 PPE-safety glasses,
 lab  coat,   latex
 gloves    (or   any
 protective  glove).
 CPE suggested

 General ventilation
 is  adeguate.   PPE-
 splash proof safety
 goggles,  lab coat,
 CPE   or    rubber
 gloves
          EPA's Characterization of Waste
SPECIAL PRECAUTIONS

Follow Biosafety Procedures  as  outlined  in the Chemical Hygiene
Safety Manual.

-------
                                                               22-1
  Laboratory Procedure;


  Method:

  Description:
 Chemicals Used:
 CHEMICAL HYGIENE SOP

        No. 22

    Determining primary  production
    parameters.

    14C Radiotracer

    Samples are inoculated with radiotracer
    14C.  The radioactivity of the filter
    containing the algal cells is determined
    by liquid scintillation counting.

   Radiotracer NaH14C03,  phenolethylamine,
   hydrochloric acid, "scintillation
   cocktail"
 Overall Glove Recommendation:   Neoprene

 SAFETY CONTROLS
 Procedure

 1.     Analytical
 procedures handling
 radioactive
 material
Potential Exposure

 Ingestion,  skin if
 not    protected
 (slight  hazard)
2.   Addition   of
hydrochloric  acid
to sample
Spills,  splashing/
inhalation
     PPE and/or
Engineering Controls

 All     radioactive
 materials  used  in
 the procedure  must
 be  done  in   the
 Designated     Area
 fume hood.  Follow
 PPE guidelines  in
 CRL     Radiation
 Safety Manual

 General ventilation
 is adequate.     If
 practical,     do
 procedure in a  fume
 hood.    PPE-splash
 proof    safety
 goggles, lab coat,
 neoprene or rubber
 gloves

-------
  Procedure

  3.   Addition   of
  liquid
  scintillation
  cocktail
       Potential Exposure

        Spills
 4.   Addition   of
 phenolethylamine
 5 .    Clean
 procedure
up
Splashing
                                       22-2
                           PPE and/or
                      Engineering Controls

                        General ventilation
                        is  adequate.  PPE-
                        safety glasses, lab
                        coat,    data    on
                        gloves
                        inconclusive,
                        recommend  viton or
                        a    viton/neoprene
                        blended     glove
                        material  .
                        Recommend  testing
                        this material prior
                        to use

                       Missing MSDS
General ventilation
is  adequate.   PPE-
safety glasses, lab
coat,     any
protective   glove.
Apron    optional.
Neoprene    gloves
suggested
WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.
SPECIAL PRECAUTIONS
Hazards from alpha particle radiation occur from ingesting.
material will block alpha particles from exposed skin.
                                             Any
Follow  all  Safety Procedures  and  Guidelines   for  radioactive
material as outlined in the CRL Radiation Safety  Manual.

-------
                                                              23-1



                       CHEMICAL HYGIENE SOP



                              No.  23



Laboratory Procedure:    Quality Control Schedule



Method:                  	



Description:              This procedure is not applicable.

-------
                                                             24-1



                       CHEMICAL HYGIENE SOP



                              No.  24



Laboratory Procedure:    Reagent Water System



Method:                  	



Description:             This procedure is not applicable.

-------
                                                              25-1
 Laboratory Procedure:



 Method:

 Description:
 CHEMICAL HYGIENE SOP

        No.  25

   Sampling surface waters for hydrophobic
   contaminants including filtration and
   liquid/liquid extraction
 Chemicals Used:
   Surface waters are collected with an
   electrically powered pump.   Filtered
   water is passed through XAD-2 Resin
   which extracts and concentrates
   hydrophobic contaminants for analysis.

   Acetone
 Overall  Glove Recommendation:   Neoprene

 SAFETY CONTROLS
Procedure

1.     Filtration
rinsing     carboys
penta  plate  parts
with    acetone
Note:  Since gloves
may     introduce
contaminants, it is
of great importance
to   decontaminate
gloves
Potential Exposure

 Spills,  splashing,
 inhalation ,
 flammable
     PPE and/or
Engineering Controls

 Acetone    is
 extremely flammable
 and  irritating  to
 skin.    Inhalation
 of large quantities
 can  be  hazardous.
 Procedure  must  be
 done   in  a   fume
 hood.       If    a
 particular
 procedure does  not
 make this feasible,
 use a local exhaust
 vent.   Vapors  can
 travel   a   great
 distance     and
 flashback    to
 source.   Under no
 circumstances
 should    ignition
 sources, sparking,
 heat  generation, be
 in the vicinity of
 these vapors.  PPE-
 splash proof safety
 goggles/ lab coat.
 Neoprene,  CPE  or
 butyl gloves

-------
                                                              25-2
                                                   PPE and/or               ^
 Procedure             Potential Exposure     Engineering Controls          •

 2.    Extraction                            Methylene  Chloride
 Methylene Chloride                            is  a known  animal
                                               carcinogen .
                                               Procedure should be
                                               pe r f orme d    in
                                               Designated   Area
                                               fume   hood.   PPE-
                                               splash proof  safety
                                               goggles, lab coat,
                                               &    neoprene   or
                                               polyvinyl  alcohol
                                               or  viton  gloves.
                                               Set  up  extraction
                                               equipment in  hood.


WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S. EPA's Characterization of  Waste
From Standard Analytical Methods.


SPECIAL PRECAUTIONS

Butyl gloves should offer excellent protection and not break  down.         ™
All fire hazards must be  removed.  Use fume hoods or local exhaust
vents that have  explosion proof motors only.  Do not do any heating
procedures near this activity.

-------
                                                               26-1
                        CHEMICAL HYGIENE SOP

                               No. 26

                           Determination  of  metals  in acid raid.

                           Inductively coupled  argon  plasma atomic
                           emission spectroscopy  (ICP-AES).

                           Acid rain  samples are  analyzed  for  15
                           different  metals.

                           Nitric acid, plasma grade  standards:
                           aluminum,  boron, barium, beryllium,
                           calcium, cobalt, copper, iron, lithium,
                           magnesium,  manganese,  strontium,
                           titanium, vanadium,  zinc

 Overall Glove Recommendation:  Chlorinated Polyethylene (CPE)

 SAFETY CONTROLS
Laboratory Procedure:

Method:


Description:


Chemicals Used:
Procedure

1.   Nitric    acid
handling,
dilutions,   sample
preservation
                       Potential Exposure

                        Spills, splashing,
                        inhalation,  heating
                                                 PPE and/or
                                            Engineering Controls

                                              Nitric   acid   is
                                              incompatible  with
                                              heat   and    most
                                              common metals  and
                                              powdered    metals.
                                              Extreme  care  must
                                              be    used    when
                                              heating.         Do
                                              procedure in a fume
                                              hood.    PPE-splash
                                              proof    safety
                                              goggles, lab coat,
                                              CPE    or     viton
                                              gloves, face shield
2.     Standard
( Standards
purchased
commercially,   not
prepared    here)
Standard    as
follows:

-------
 Procedure

 2a.  Aluminum
Potential Exposure

 Inhalation
 2b.  Barium
 Inhalation
 2c. Beryllium
 Inhalation, spills
2d. Boron
Inhalation
2e.  Calcium
None
                26-2
     PPE and/or
Engineering C  trols
         V
  General ventilation
  adequate.      PPE-
  splash proof safety
  goggles,  lab coat,
  rubber gloves (data
  inconclusive    on
  gloves).        CPE
  adequate

  General ventilatin
  adequate.      PPE-
  splash proof safety
  goggles, lab  coat,
  rubber gloves (data
  inconclusive     on
  gloves).       CPE
  adequate

 This material  is a
 carcinogen .
 Procedure  must  be
 done     in    the
 Designated    Area
 fume  hood.     PPE-
 splash proof safety
 goggles, lab coat,
 rubber    gloves
 (double    gloving
 recommended).   CPE
 adequate

 General  ventilation
 adequate.      PPE-
 splash proof safety
 goggles, lab coat,
 rubber gloves (data
 inconclusive    on
 gloves).        CPE
 adequate

 General  ventilatin
 adequate.      PPE-
 splash proof safety
 goggles,  lab coat,
 rubber gloves (data
 inconclusive    on
 gloves).        CPE
 adequate

-------
 Procedure

 2£. Silver
Potential Exposure

 Inhalation, spills
 2g.  Cobalt
 Inhalation
 2h. Copper
 Inhalation
2i. Iron
Inhalation
2j. Lithium
Inhalation
                26-3
     PPE and/or
Engineering Controls

  General ventilation
  adequate.      PPE-
  splash proof safety
  goggles,  lab coat,
  rubber gloves (data
  inconclusive    on
  gloves).        CPE
  adequate

  General ventilation
  adequate.      PPE-
  splash proof safety
  goggles, lab  coat,
  rubber gloves (data
  inconclusive     on
 gloves).       CPE
 adequate

 General  ventilation
 adequate.      PPE-
 splash proof safety
 goggles,  lab coat,
 rubber gloves  (data
 inconclusive    on
 gloves).        CPE
 adequate

 General ventilation
 adequate.      PPE-
 splash proof safety
 goggles,  lab coat,
 rubber gloves (data
 inconclusive    on
 gloves).        CPE
 adequate

 General ventilation
 adequate.      PPE-
 splash proof safety
 goggles, lab  coat,
 rubber gloves (data
 inconclusive    on
 gloves).        CPE
 adequate

-------
 Procedure

 2k. Magnesium
Potential Exposure

 Inhalation
 21.  Manganese
 Inhalation
 2m.  Strontium
 Inhalation, spills
2n. Titanium
Inhalation, spills
2o. Vanadium
Inhalation,  spills
                26-4
     PPE and/or
Engineering Controls

  General ventilation
  adequate.      PPE-
  splash proof safety
  goggles,  lab coat,
  rubber gloves (data
  inconclusive    on
  gloves) .        CPE
  adequate

  General ventilation
  adequate.      PPE-
  splash proof safety
  goggles, lab  coat,
  rubber gloves (data
  inconclusive     on
 gloves).       CPE
 adequate

 General ventilation
 adequate.     PPE-
 splash proof safety
 goggles, lab coat,
 rubber gloves  (data
 inconclusive    on
 gloves).        CPE
 adequate

 General ventilation
 adequate.      PPE-
 splash proof safety
 goggles,  lab coat,
 rubber gloves (data
 inconclusive    on
 gloves).        CPE
 adequate

 General ventilation
 adequate.      PPE-
 splash proof safety
 goggles,  lab coat,
 rubber gloves (data
 inconclusive   on
 gloves).        CPE
 adequate

-------
Procedure

2p. Zinc
                                Exposure
                       Inhalation, spills
    AROO
3.
preparation
nitric acid
standard
      in
Spills, splashing,
inhalation, heating
4.  AR01   Standard
preparation ,
calcium   magnesium
in nitric  acid
                       Spills,  splashing,
                       inhalation, heating
                                                   26-5
                                       PPE  and/or
                                  Engineering Controls

                                    General ventilation
                                    adequate.      PPE-
                                    splash proof safety
                                               goggles,
.,__,_,_  .  lab coat,
rubber gloves (data
inconclusive    on
gloves).        CPE
adequate

Nitric   acid   is
incompatible  with
heat   and   most
common metals  and
powdered   metals.
Extreme  care  must
be    used    when
heating.        Do
procedure in a fume
hood.    PPE-splash
proof     safety
goggles,  lab coat,
CPE    or   rubber
gloves   (acid   is
diluted)

Nitric .  acid   is
incompatible  with
heat    and   most
common  metals  and
powdered   metals.
Extreme  care  must
be    used    when
heating.        Do
procedure in a fume
hood.    PPE-splash
proof    safety
goggles, lab coat,
CPE    or   rubber
gloves   (acid  is
diluted)

-------
 Procedure

 5.   AR02  standard
 preparation,
 aluminum,    boron,
 barium,  beryllium,
 cobalt,     copper,
 iron,    lithium,
 manganese ,
 strontium,
 titanium, vanadium,
 and  zinc in nitric
 acid
Potential Exposure
 I n h a 1 a
 splashing
t i o n
6.  Interelemental
correction
solution,  iron  in
nitric acid
Spills,  splashing,
inhalation,  heating
                26-6
     PPE and/or
Engineering Controls

  Beryllium    is    a
  carcinogen ,
  therefore ,
  procedure  must  be
  done    in    the
  Designated   Area
  fume hood.   Nitric
  acid      is
  incompatible  with
  heat    and   most
  common    metals.
  Extreme  care must
  be    used    when
  heating.        Do
 procedure in  a fume
 hood.    PPE-splash
 proof     safety
 goggles,  lab coat,
 CPE    or    rubber
 gloves,  (acid  is
 diluted)

 Nitric    acid   is
 incompatible   with
 heat    and    most
 common  metals  and
 powdered    metals.
 Extreme care  must
 be    used     when
 heating.         Do
 procedure in a fume
 hood.    PPE-splash
 proof     safety
 goggles,  lab  coat,
 CPE    or   rubber
 gloves,   (acid   is
 diluted)

-------
 Procedure

 7.   Lab   control
 standards, high and
 low,    aluminum,
 boron,     barium,
 beryllium, cobalt,
 copper,    iron,
 lithium, manganese,
 strontium,
 titanium,  vanadium,
 and zinc in nitric
 acid
8.     Intermediate
solution    and
dilution, aluminum,
iron,    magnesium,
boron,    copper,
titanium, vanadium,
zinc,    barium,
lithium, manganese,
strontium,
beryllium,  calcium,
cobalt   in  nitric
acid
Potential Exposure

 Inhalation
 splashing
Inhalation
splashing
                26-7
     PPE and/or
Engineering Controls

  Beryllium   is   a
  carcinogen ,
  therefore ,
  procedure must  be
  done    in    the
  Designated    Area
  fume hood.   Nitric
  acid     is
  incompatible   with
  heat    and    most
  common  metals and
  powdered   metals.
  Extreme  care  must
  be    used    when
  heating.        Do
 procedure in  a fume
 hood.    PPE-splash
 proof     safety
 goggles, lab coat,
 CPE   or    rubber
 gloves,   (acid  is
 diluted)

 Beryllium   is   a
 carcinogen ,
 therefore,
 procedure  must  be
 done    in     the
 Designated    Area
 fume hood.   Nitric
 acid      is
 incompatible   with
 heat    and    most
 common    metals.
 Extreme  care  must
 be    used    when
 heating.        Do
 procedure in a fume
 hood.    PPE-splash
proof     safety
goggles,  lab coat,
CPE    or   rubber
gloves    (acid  is
diluted)

-------
                                                              26-8
                                                   PPE and/or
 Procedures             Potential Exposure     Engineering Controls

 9.    Analytical      Inhalation              Do  as   much   as
 procedures ,                             practical under  a
 calibrations,                                  fume   hood  or   a
                                                local exhaust vent.
                                                Avoid     breathing
                                                fumes.   PPE-safety
                                                glasses  or  splash
                                                proof goggles,  lab
                                                coat, rubber or CPE
                                                gloves
 WASTE GENERATION AND HANDLING

 Follow all guidelines in the  U.S. EPA's Characterization of Waste
 From Standard Analytical Methods.


 SPECIAL PRECAUTIONS

 Many  standards used  are carcinogenic  or  very poisonous.   Extreme
 care  must be  taken  when handling  these  materials.    Follow  all
 procedures  carefully in the analytical procedure  manual.   Double
 gloving  is  recommended.

 Nitric  acid used  in these  procedures is  dilute  enough to most
 likely not react with metals and heat.  However, care must still be
 used.

Wash hands thoroughly after procedure  completion.

-------
 Laboratory Procedure:


 Method:

 Description:


 Chemicals Used:
                                                              27-1
 CHEMICAL HYGIENE SOP

        No.  27

   Determination of cadmium, chromium,
   and nickel in acid rain samples.

   Flameless atomic absorption spectroscopy

   Acid rain samples are analyzed by
   flameless atomic absorption

   Nitric acid,  ammonium phosphate,
   magnesium nitrate, stock standards:
   cadmium,  chromium, nickel,  argon
 Overall Glove Recommendation:   Chlorinated Polyethylene  (CPE)

 SAFETY CONTROLS
 Procedure

 1 .     Sample
 preservation  with
 nitric acid
Potential Exposure

 Spills,  splashing,
 inhalation
2. Matrix modifier:
ammonium   hydrogen
phosphate  ,
magnesium   nitrate
hexahydrate
solution
preparation
None
     PPE and/or
Engineering Controls

  Nitric    acid   is
  incompatible   with
  heat   and   common
  metals and powdered
  metals.     Extreme
  care must be taken.
  Do  procedure in  a
  fume  hood.    PPE-
  splash proof safety
  goggles,  lab  coat,
  viton,     rubber
  gloves,   or   face
  shield    optional.
 CPE adequate

 General ventilation
 is  adequate.    If
 practical,    do
 procedure in a fume
 hood.    PPE-splash
 proo f     safety
 goggles,  lab coat,
 any    protective
 glove   recommended
 (data  inconclusive
 on  gloves) .    CPE
 adeauate

-------
 Procedure

 3.   Flasks   stored
 with nitric  acid
Potential Exposure

 Spills,  splashing,
 inhalation
4.  Stock  standard,
stock   calibration
standards handling:
cadmium
 Spills, inhalation,
 contact
4a. Chromium
 Spills, inhalation,
 contact
4b. Nickel
Inhalation
                27-2
     PPE and/or
Engineering Controls

  Nitric    acid   is
  incompatible   with
  heat   and   common
  metals and powdered
  metals.     Extreme
  care must be taken.
  Do  procedure in  a
  fume  hood.    PPE-
  splash proof safety
  goggles,  lab  coat,
  viton  gloves,  CPE
  or  rubber  gloves,
  face    shield
  optional

  Do  procedure  in  a
  fume  hood.   PPE-
  splash proof safety
  goggles, lab  coat,
  (no data  on  glove
 types)   recommend
 rubber    or   CPE
 gloves

 This material is  a
 carcinogen.     Do
 procedure  in  the
 Designated    Area
 fume  hood.    PPE-
 splash proof safety
 goggles, lab coat:,
  (no data on gloves)
 recommended rubber
 or CPE  gloves and
 double gloving

 This material is  a
 carcinogen  .
 Procedure must  be
 done    in    the
 Designated    Area
 fume  hood.    PPE-
 splash proof safety
 goggles,  lab coat,
 CPE    or    rubber
 gloves plus double
 gloving recommended

-------
 Procedure

 5.   Dilution    of
 standards    with
 nitric acid
         Potential Exposure

          Spills, splashing,
          inhalation
 6.     Working
 calibration
 standards
 preparation-
          Spills,  splashing,
          inhalation
 dilution
 nitric acid
with
 7 .     Ins trument
 calibration
         Spills, inhalation,
         eyes
8.    Analytical
procedures
         Spills,  inhalation
                27-3
     PPE and/or
Engineering Controls

  Do procedure  in  a
  fume  hood.    PPE-
  splash proof safety
  goggles,  lab coat,
  viton  gloves,  CPE
  or rubber  gloves,
  face    shield
  optional

  Do  procedure in  a
  fume  hood.     PPE-
  splash proof safety
 goggles, lab coat,
 viton  gloves,  CPE
 or  rubber  gloves,
 face    shield
 optional

 Do  as   much   of
 procedure    as
 possible in  a fume
 hood.         Local
 exhaust    over
 instrument  may  be
 more    practical.
 PPE-safety  glasses
 or  splash   proof
 safety goggles, lab
 coat, rubber or CPE
 gloves,  proper  eye
 protection     for
 lighting
 electrodeless
 discharge lamp

 Do   as   much   of
 procedure    as
 possible in  a fume
 hood.         Local
 exhaust     over
 instrument may  be
more    practical.
PPE-safety glasses
or   splash    proof
safety goggles,  lab
coat, rubber  or CPE
gloves

-------
WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.


SPECIAL PRECAUTIONS

Nitric acid  is  diluted enough  to  reduce potential for  heat  and
metal  reactions.    However,  care  must  still  be  used.    Metal
standards are toxic.  Use extreme care with  carcinogens.   Double
gloving recommended for spills.

-------
                                                              28-1
                        CHEMICAL HYGIENE SOP

                               No.  28

                          Determination of total arsenic in water,

                          Hydride generation atomic absorption
                          spectroscopy.

                          Samples are digested and oxidized.
                          Arsenic is determined spectrophoto-
                          metrically.

                          Potassium persulfate,  nitric  acid/
                          sodium borohydride,  hydrochloric  acid,
                          sodium hydroxide/  arsenic  standard/
                          arsenic pentoxide  standard/ dimethyl
                          arsenic acid (cacodylic  acid), arsenic
                          trioxide  standard

Overall Glove Recommendation:   Chlorinated Polyethylene (CPE)

SAFETY CONTROLS
Laboratory Procedure:

Method:


Description:



Chemicals Used:
Procedure

1.   Nitric   acid
additions to sample
bottles
                      Potential Exposure

                       Spills/  splashing/
                       inhalation
     PPE and/or
Engineering Controls

  Nitric    acid   is
  incompatible   with
  heat   and   common
  metals and powdered
  metals.     Extreme
  care must be taken.
  Do  procedure in  a
  fume  hood.    PPE-
  splash proof safety
  goggles/  lab  coat/
  viton  gloves,  CPE
  or  rubber  gloves,
  face     shield
  optional

-------
 Procedure
 2 .    Sodium
 borohydride
 solution,    mixed
 with    sodium
 hydroxide
Potential Exposure
 Spills, splashing,
 inhalation
 3.    Potassium
 persulfate solution
                28-2
     PPE and/or
Engineering Controls
  Sodium borohydride
  will    liberate
  hydrogen   gas   on
  contact with water.
  Sodium   hydroxide
  imay    generate
  sufficient heat  to
  ignite combustibles
  when   in  contact
  with    water.
  Procedure  must  be
  done   in   a   fume
  hood.   PPE-splash
  proof    safety
  goggles,     face
  shield recommended,
  lab  coat,   rubber
  apron  recommended,
  rubber  gloves are
  only  common   glove
  for both materials.
 CPE gloves adequate

 Local  exhaust  is
 adequate.     PPE-
 splash proof safety
 goggles,  lab  coat,
 CPE,   rubber   or
 neoprene   gloves.
 Potassium
 persulfate  is  an
 oxidizer, keep from
 £lammables
4.    Arsenic
standards
preparation:

4 a .    Arsenic
trioxide,   nitric
acid
Spills,  splashing,
inhalation
Arsenic     is     a
carcinogen.    This
material    reacts
with  acids.     Do
procedure    with
extreme   caution.
Procedure  must  be
done    in    the
Designated    Area
fume hood.  PPE-

-------
 Procedure
Potential Exposure
 4b. Arsenic spiking
 solution,    nitric
 acid
 Spills,  splashing,
 inhalation
4 c .    Arsenic
pentoxide,   sodium
hydroxide
Spills, splashing,
inhalation
                28-3
     PPE and/or
Engineering Controls

  splash proof safety
  goggles,    face
  shield,  lab  coat,
  rubber    apron,
  rubber gloves. CPE
  adequate

  Arsenic    is    a
  carcinogen.   This
  material    reacts
  with  acids.     Do
  procedure    with
  extreme   caution.
  Procedure must  be
 done     in    the
 Designated    Area
 fume  hood.    PPE-
 splash proof safety
 goggles,     face
 shield,   lab  coat,
 rubber    apron,
 rubber   or    CPE
 gloves

 Sodium   hydroxide
 may     cause
 sufficient  heat to
 ignite combustibles
 when  in   contact
 with    water.
 Arsenic     is    a
 carcinogen.    This
 material    reacts
 with  acids.    Do
 procedure    with
 extreme    caution.
 Procedure  must be
 done    in    the
 Designated    Area
 fume  hood.   PPE-
 splash proof safety
 goggles,     face
 shield,  lab  coat,
 rubber    apron,
rubber    or    CPE
gloves

-------
 Procedure

 4d.   Arsenic   (V)
 intermediate   and
 standard,   nitric
 acid
Potential Exposure

 Spills,  splashing,
 inhalation
 4e.    Dimethyl
 arsenic    acid,
 sodium   hydroxide,
 stock and  spiking
Spills,  splashing,
inhalation
5.    Instrument
operation    and
calibration
Spills,  inhalation
                28-4
     PPE and/or
Engineering Controls

  Arsenic    is    a
  carcinogen.    This
  material    reacts
  with   acids.     Do
  procedure    with
  extreme    caution.
  Procedure  must  be
  done    in    the
  Designated    Area
  fume  hood.    PPE-
  splash proof safety
  goggles, face
  shield,  lab coat,
  rubber    apron,
  rubber    or    CPE
  gloves

 This material is a
 carcinogen.  Sodium
 hydroxide   reacts
 with water and may
 generate  sufficient
 heat   to   ignite
 combustibles.    Do
 procedure  in  the
 Designated    Area
 fume  hood.    PPE-
 splash proof safety
 goggles,,     face
 shield,  lab  coat,
 rubber    apron,
 rubber  gloves  or
 CPE  adequate

 Do   as   much   of
 procedure    as
 possible  in  a fume
 hood.  Or use local
 exhausting    over
 operation.     PPE-
 splash proof safety
 goggles,  lab  coat,
 CPE    or    rubber
 gloves.   Wear  eye
 protection  for  UV
 light when igniting
 electrodeless
 discharge lamp

-------
  procedure

  6 .     Digestion
  procedure
Potential Exposure

 Spills,  inhalation,
 heat (burns)
 7.    Analytical
 procedure
Spills, inhalation
 8.    Rinsing   and
 adding  hydrochloric
 acid
Spills, splashing,
inhalation
                28-5
     PPE and/or
Engineering Controls

  Use  local  exhaust
  over    autoclave.
  Avoid     breathing
  vapors.      PPE-as
  outlined for  each
  solution    and
  standard.       Use
  insulated    gloves
  handling hot items.
 Allow  a  clean  air
 purge    before
 opening autoclave

 Do   as   much   of
 procedure    as
 possible in a fume
 hood.  Or use local
 exhaust    over
 operation.  PPE-as
 outlined  for  each
 standard

 May  cause  severe
 skin  or respiratory
 burns.         Do
 procedure in a fume
 hood.    PPE-splash
 proof     safety
 goggles,  lab coat,
 CPE,  neoprene   or
 rubber  gloves
WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.
SPECIAL PRECAUTIONS
Use proper  procedures handling carcinogens and  toxic materials
Allow for thorough decontamination.

-------
                                                              29-1
 Laboratory Procedure:


 Method:

 Description:
                       CHEMICAL HYGIENE SOP

                              No.  29

                         Determination of total potassium and
                         sodium in acid rain.

                         Flame atomic absorption

                         Acid rain samples are analyzed by
                         atomic absorption.
                         Nitric acid, potassium standards,
                         sodium standards, hydrochloric acid
Chemicals Used:


Overall Glove Recommendation:   Chlorinated  Polyethylene  (CPE)
 SAFETY  CONTROLS
 Procedure

 1  .    Sample
 preservation   with
 nitric acid
                      Potential Exposure

                       Spills,  splashing,
                       inhalation
2.    Potassium
standards ,
intermediate
standards, working
standards in nitric
acid
                      Spills,  splashing,
                      inhalation
     PPE and/or
Engineering Controls

  Nitric    acid   is
  incompatible  with
  heat, common metals
  and    p owd e r ed
  metals.     Extreme
  care must be taken.
  Do  procedure  in  a
  fume  hood.    PPE-
  splash proof safety
  goggles,  lab coat,
  CPE,    viton    or
  rubber gloves, face
  shield optional

  Nitric   acid   is
  incompatible   with
  heat, common metals
  and    powde red
 metals.    Extreme
  care must be  taken.
  Do  procedure in  a
  fume  hood.    PPE-
  splash proof  safety
 goggles, lab  coat,
 CPE,    viton   or
 x-ubber gloves, face
  shield optional

-------
  Procedure

  3  .    Sodium
  standards  ,
  intermediate
  standards, working
  standards,    in
  nitric acid
 Potential Exposure

 Spills,  splashing,
 inhalation
 4.    Instrument
 calibration/ burner
 operation
 Heat     (burns),
 compressed    gas
 cylinder explosions
 5.     Ins trument
 calibration
Inhalation, spills
6 .    Analytical
procedure  washing
with  hydrochloric
acid
Spills,  splashing,
inhalation
                29-2
     PPE and/or
Engineering Controls

  Nitric    acid   is
  incompatible   with
  heat, common metals
  and    powd e r e d
  metals.     Extreme
  care must be taken.
  Do  procedure in  a
  fume  hood.     PPE-
  splash proof safety
  goggles,  lab  coat,
  CPE,    viton   or
  rubber gloves, face
  shield optional

 Keep  combustibles
 away from operating
 area.     Acetylene
 tank must be secure
 and   upright.
 Follow all  safety
 procedures    in
 Compressed     Gas
 Association
 Pamphlet P-l

 Do    as    many
 procedures     as
 possible in  a  fume
 hood.  Or use local
 exhausting   during
 operation.     PPE-
 splash proof  safety
goggles, lab coat,
CPE, rubber gloves

May  cause   severe
skin or respiratory
burns.        Do
procedure in  a  fume
hood.   PPE-splash
proof     safety
goggles,  lab coat,
CPE,   neoprene  or
rubber  gloves

-------
                                                             29-3

WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.


SPECIAL PRECAUTIONS

None
                                                                         I

-------
                                                               30-1
 Laboratory Procedure:

 Method:

 Description:
 CHEMICAL HYGIENE SOP

        No.  30

   Determination of nickel in acid rain.

   Flameless atomic absorption

   Acid rain samples are analyzed by atomic
   absorption.
 Chemicals Used:          Nitric acid, nickel standards

 Overall Glove Recommendation:  Chlorinated Polyethylene (CPE)

 SAFETY CONTROLS
 Procedure

 1 .     Sample
 preservation  with
 nitric    acid,
 rinsing,  immersions
Potential Exposure

 Spills,  splashing,
 inhalation
2. Nickel standard,
stock   calibration
standard,   working
calibration
sta.ndard
preparation   with
nitric acid
Spills,  splashing,
inhalation
     PPE and/or
Engineering Controls

  Nitric    acid   is
  incompatible   with
  heat, common metals
  and    powdered
  metals.     Extreme
  care must be taken.
  Do  procedure in  a
  fume  hood.    PPE-
  splash proof safety
  goggles,  lab  coat,
 CPE,    viton    or
 rubber gloves, face
 shield optional

 Nitric   acid    is
 incompatible  with
 heat,     common
 metals,    and
 powdered   metals.
 Nickel    is    a
 carcinogen.   This
 procedure  must  be
 done    in    the
 Designated    Area
 fume hood.    PPE-
 splash proof safety
 goggles,  lab coat,
 CPE, rubber gloves,
 double    gloving
 recommended

-------
 Procedure

 3.    Instrument
 calibration
Potential Exposure

 Spills,  inhalation,
 eyes
4.     Analytical
procedures
Spills, inhalation
WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S.
From Standard Analytical Methods,
                30-2
     PPE and/or
Engineering Controls

  Do   as   much   of
  procedure    as
  possible  in a  fume
  hood    or    local
  exhaust   over
  operation.     PPE-
  safety  glasses or
  splash proof safety
  goggles,  lab coat,
  CPE    or    rubber
  gloves, proper eye
 protection    for
  lighting
 electrodeless
 discharge  lamp

 Do  as    much   of
 procedure     as
 possible in a fume
 hood    or   local
 exhaust     over
 operation.    PPE-
 safety  glasses  or
 splash proof safety
 goggles, lab  coat,
 CPE    or    rubber
 gloves
           EPA's  Characterization of Waste
                                                                        I
SPECIAL PRECAUTIONS

Double gloving recommended for carcinogens in the event of a spill.
Use care in handling carcinogens.

-------
                                                              31-1
 Laboratory Procedure:
 CHEMICAL HYGIENE SOP

        No. 31

   Analysis of  total Kjeldahl nitrogen
   and  total  phosphorous in water.
 Method:                  	

 Description:             Samples are evaporated and digested.  They
                          are then analyzed for total phosphorous
                          and total Kjeldahl nitrogen using a
                          technicon auto analyzer.

 Chemicals Used:           Sulfuric acid/ potassium sulfate, mercuric
                          oxide,  sodium hydroxide, potassium sodium
                          tartrate,  sodium  citrate, phenol,  bleach
                          (household), sodium nitroprusside, sodium
                          chloride,  ascorbic acid, potassium anti-
                          mony 1 tartrate, adenosine-5' monophos-
                          phoric  acid, sodium salt, potassium
                          sulfate,  ammonium molybdate, sodium
                          hypochlorite, ascorbic acid, glutamic
                          acid, potassium dihydrogen phosphate

Overall Glove Recommendation:  Chlorinated Polyethylene  (CPE)
SAFETY CONTROLS
Procedure

1.     Digesting
solution
preparation
Potential Exposure

 Spills,  splashing,
 inhalation
     PPE and/or
Engineering Controls

  Do  procedure  in  a
  fume    hood.
  Mercuric  oxide  is
  extremely    toxic,
  sulfuric    acid
  reacts    violently
  with  water.   Pour
  acid  slowly.   PPE-
  splash proof safety
  goggles, lab  coat,
  viton,  chlorinated
  polyethylene    or
  polyethylene gloves
  and  apron.    When
  handling potassium
  sulfate  only,  any
  protective    glove
  can be  used.    CPE
  suggested

-------
 Procedure

 2.    Digesting
 solution,  glassware
 cleaning
 3.    Sampler   wash
 solution
 preparation
 4.   Dilution  loop
 solution
 preparation
 5.     Complexing
 reagents   solution
 preparation
6. Alkaline  phenol
                  n
preparation
Potential Exposure

 Spills/  splashing/
 inhalation
 Spills/  splashing,
 inhalation
 Spills/  splashing,
 inhalation
None documented
Spills, splashing,
inhalation
                31-2
     PPE and/or
Engineering Controls

  Do procedure  in  a
  fume  hood.    PPE-
  splash proof safety
  goggles,  lab coat,
  CPE,  neoprene  or
  rubber gloves

  Do procedure  in  a
  fume  hood.    PPE-
  splash proof safety
  goggles,  lab coat,
  viton, chlorinated
  polyethylene,    or
  polyethylene gloves

  Do   procedure   in
  fume  hood.   PPE-
  splash proof safety
 goggles,   lab coat,
 nitrile,   neoprene,
 CPE,   rubber   or
 polyethylene gloves

 General ventilation
 is adequate.   PPE-
 splash proof safety
 goggles,  lab coat,
 any    protective
 glove.         CPE
 suggested

 Do procedure  in  a
 fume hood.  Phenol
 and    sodium
 hydroxide    are
 extremely
 poisonous.    PPE-
 splash proof safety
 goggles,  lab coat,
 CPE or butyl gloves
7 .     Sodium
hypochlorite
solution
preparation
Spills,  splashing,
inhalation
General ventilation
adequate.  If  prac-
tical/  do in  fume
hood.    PPE-splash
proof    safety
goggles/  lab  coat/
CPE/    viton/    or
rubber  gloves

-------
 Procedure

 8.   Sodium  nitro-
 prusside    reagent
 preparation
Potential Exposure

 Spills,  splashing/
 inhalation
 9. Sodium chloride
 solution
 preparation
 None
 10.   Sulfuric  acid
 solution
 preparation
 Spills,  splashing,
 inhalation
 11 .    Ammon ium
 molybdate  solution
 preparation
Spills,  splashing,
inhalation
12.  Ascorbic  acid
solution
preparation
None
                31-3
     PPE and/or
Engineering Controls

  Sodium    nitro-
  prusside    is    a
  poison.     Perform
  procedure  in  fume
  hood.   PPE-splash
  proof    safety
  goggles, lab  coat,
  CPE, rubber gloves

  General ventilation
  is adequate. No PPE
  required.    Splash
  proof    safety
  goggles, lab coat,
  any    protective
 glove.        CPE
 suggested

 Do  procedure   in
 fume  hood.    Add
 acid carefully  to
 avoid    violent
 reaction    with
 water.   PPE-splash
 proof    safety
 goggles, lab coat,
 viton,  chlorinated
 polyethylene gloves
 and  apron.    Apron
 optional

 General ventilation
 is  adequate.   If
 practica1,    do
 procedure in a fume
 hood.    PPE-splash
 proof     safety
 goggles, lab coat,
 CPE    or    rubber
 gloves

General  ventilation
 is adequate.  PPE-
splash proof safety
goggles, lab coat,
any    protective
glove.         CPE
suggested

-------
 Procedure

 13.    Potassium
 antimonyl tartrate
 solution
 preparation
 14. Combined color
 reagent preparation
Potential Exposure

 Spills, splashing,
 inhalation
 Spills,  splashing,
 inhalation
 15.  Nitrogen stock
 standard
 preparation
 Spills,  splashing,
 inhalation
16.    Phosphorous
stock    standard
preparation,
working     range
dilutions
Spills,  splashing,
inhalation
17.    Stock   AQC
standard,  working
standard dilutions
Spills,  splashing,
inhalation
                31-4
     PPE and/or
Engineering Controls

  General ventilation
  is  adequate.   PPE-
  splash proof safety
  goggles,  lab  coat,
  CPE    or    rubber
  gloves

  General ventilation
  is  adequate.     If
  practical,    do
  procedure  in  fume
  hood.      Combine
  reagents  in   order
  given in Laboratory
 Procedures.    PPE-
 splash proof safety
 goggles, lab  coat,
 viton, chlorinated
 polyethylene,
 polyethylene gloves

 Do procedure  in  a
 fume  hood.    PPE-
 splash proof safety
 goggles,  lab coat,
 viton,  chlorinated
 polyethylene,
 polyethylene gloves

 General ventilation
 is adequate.   PPE-
 splash proof safety
 goggles,  lab  coat,
 viton,  chlorinated
 polyethylene,
 polyethylene
 gloves.   Use  same
 PPE   to   preserve
 samples    with
 sulfuric acid

 General ventilation
 is  adequate.    Do
 procedure  in  fume
 hood  if  practical.
 PPE-splash   proof
 safety goggles, lab
 coat, viton,

-------
 Procedure
Potential Exposures
 18.    Digestion,
 glassware    rinse
 with   hydrochloric
 acid
 Spills,  splashing,
 inhalation
 19.    Sample
 preparation,
 calibration,
 pumping  reagents,
 handling   samples
 during  tests

 20.     Evaporating
 sample  tubes
Spills,  splashing,
inhalation
Inhalation,
(burns)
heat
WASTE GENERATION AND HANDLING
                        31-5
             PPE and/or
        Engineering Controls

          chlorinated
          polyethylene,
          polyethylene gloves

          If   practical,   do
          procedure  in  fume
          hood.       General
          ventilation    is
          adequate.      PPE-
          splash proof safety
          goggles, lab coat,
          rubber     (choice)
          gloves  and  apron,
          CPE    or    viton,
          gloves (adequate)

         Use appropriate PPE
         when    handling
         calibration
         standards samples
Use     care    in
operating   heating
equipment.     Use
exhaust   hood  or
vent    while
                                              operating.
                                              carefully
                                              removing
                                   Handle
                                    while
Follow all guidelines in the U.S. EPA's Characterization  of  Waste
From Standard Analytical Methods.
SPECIAL PRECAUTIONS

Use extreme care in handling phenol and mercuric oxide.  These are
extremely toxic.  Use care in  handling the many, different acids.

-------
                                                               32-1
 Laboratory Procedure:


 Method:

 Description:
                       CHEMICAL HYGIENE SOP

                              No. 32

                         Analysis of  particulate  organic  carbon
                         in  lake water.

                         Infrared defection of carbon dioxide.

                         Non-dissolved carbon in  water is caught
                         on  a fiberglass filter which is burned
                         to  carbon dioxide.  Carbon dioxide is
                         sparged and quantified by an IR detector.
                          Potassium hydrogen phthalate standard,
                          sulfuric acid,  EDTA standard
Chemicals Used:


Overall Glove Recommendation:  Chlorinated Polyethylene (CPE)
 SAFETY CONTROLS
 Procedure

 1.  Stock potassium
 hydrogen phthalate
 standard,   mix  in
 sulfuric acid
                      Potential Exposure

                       Spills,  splashing,
                       inhalation
2.     Working
calibration
standards
                       Spills,  splashing,
                       inhalation
     PPE and/or
Engineering Controls

 General ventilation
 is   adequate   for
 small   volume   of
 acid.        If
 practical,     do
 procedure  in  fume
 hood.    PPE-splash
 proof    safety
 goggles, lab  coat,
 viton,  chlorinated
 polyethylene,
 polyethylene gloves

 General ventilation
 is   adequate   for
 small   volume   of
 acid.        If
 practical,    do
 procedure  in  fume
 hood.   PPE-splash
 proof    safety
 goggles, lab coat,
 viton,  chlorinated
 polyethylene,
 polyethylene gloves

-------
  Procedure

  3.   EDTA  standard
  preparation, mix in
  sulfuric acid
 Potential  Exposure

  Spills,  splashing,
  inhalation
 4.   EDTA   control
 standard  high  and
 low
 Moderate splash
 5.  Instrument start
 up,    oxygen
 adjustment
 Compressed     gas
 cylinder explosion
6. Instrument start
up,  furnace  start
up

7. Instrument start
up,   addition   of
sulfuric acid
Flammable
Spills,  splashing,
inhalation
                32-2
     PPE and/or
 Engineering Controls

  General ventilation
  is   adequate   for
  small  volume   of
  acid.        If
  practical,    do
  procedure in  fume
  hood.   PPE-splash
  proof    safety
  goggles,  lab  coat,
  viton,  chlorinated
  polyethylene,
  polyethylene gloves

  General ventilation
  is   adequate  for
  small   volume  of
  acid.         If
  practical,    do
  procedure  in  fume
  hood.   PPE-splash
  proof     safety
  goggles,  lab coat,
  neoprene,  rubber or
 CPE gloves

 Keep    cylinder
 upright and secure.
 Keep all f lammables
 away  from   area.
 Follow safety rules
 in  Compressed  Gas
 Association
 Pamphlet P-l

 Keep   combustibles
 and  oxygen  tanks
 safely away

 General ventilation
 is   adequate  for
 small   volume   of
 acid.         If
 practical,    do
 procedure  in  fume
 hood.   PPE-splash
 proof     safety
 goggles,  lab coat,
viton,  chlorinated
polyethylene,
polyethylene gloves

-------
 Procedure

 8.  Preparation  of
 nickel   shells   in
 furnace
 9. Sample analysis
Potential Exposure

 Heat (burns)
 Inhalation
                32-3
     PPE and/or
Engineering Controls

  Use    insulated
  gloves  and forceps
  handling hot items.
  Use  local  exhaust
  during  operation

  Use  local  exhaust
  during  operation.
  PPE-as outlined for
  each standard
WASTE GENERATIONS AND HANDLING

Follow all guidelines in the U.S.  EPA's Characterization of  Waste
From Standard Analytical Methods.
SPECIAL PRECAUTIONS

Use proper procedures handling compressed  gas cylinders.

-------
                                                              33-1
 Laboratory Procedure:
 Method:

 Description:
 Chemicals Used:
  CHEMICAL HYGIENE SOP

         No.  33

    Direct observation of bacteria by
    DAPI.
    A sample is prepared on a microscope slide
    and bacteria counted  under  a  compound
    microscope.

    Gluteraldehyde,  nitric acid,  irgalan
    black  (stain), 4',  6-diamidine-2
    phenylindol,  acetic acid, paraffin  oil,
    ethyl  alcohol
 Overall Glove Recommendation:   Chlorinated Polyethylene  (CPE)

 SAFETY CONTROLS
 Procedure
 1  .     Sample
 preservation   with
 gluteraldehyde
Potential Exposure
  Spills,  splashing,
  inhalation
2.    Wash    all
glassware    with
nitric acid
 Spills, splashing,
 inhalation
      PPE and/or
Engineering Controls
  Glutaraldehyde
  readily  penetrates
  skin.  Do procedure
  in  a fume hood  if
  at  all  practical.
  General ventilation
  may    only    be
  adequate   if   low
  concentrations  are
  used.    PPE-splash
  proof    safety
  goggles, lab  coat,
  neoprene  or   butyl
  gloves.        CPE
  adequate

  Nitric   acid   is
  incompatible   with
  heat,    common
  metals,   powdered
  metals.    Extreme
  care must be taken.
  Do procedure  in a
  fume  hood.    PPE-
  splash proof safety
  goggles,  lab coat,

-------
  Procedure
Potential Exposure
 3.   Irgalan   black
 stain  mixed   with
 acetic acid
 Spills, splashing,
 inhalation
 4. 4',  6-diamidino-
 2 -phenylindol
 (DAPI),   solution
 preparation
 Spills,  splashing,
 inhalation
 5.   Staining   and
 filtering    DAPI
 solution
 Spills,  splashing,
 inhalation
6.   Staining   and
filtering,  Irgalan
black staining
Spills, splashing,
inhalation
7  .     Slid
preparation
None
                33-2
     PPE and/or
Engineering Controls

  CPE,    viton    or
  rubber gloves, face
  shield optional

  If  practical,   do
  procedure in a fume
  hood.    PPE-splash
  proof    safety
  goggles, lab  coat,
  neoprene    or
  chlorinated
  polyethylene gloves

  Do procedure  in a
  fume  hood.    PPE-
 splash proof safety
 goggles,  lab coat,
 heavy   rubber  or
 double    rubber
 gloving.        CPE
 adequate

 If   practical,  do
 procedure in a fume
 hood.    PPE-splash
 proof    safety
 goggles,  lab coat,
 heavy   rubber   or
 double    rubber
 gloving.        CPE
 adequate

 If  practical,   do
 procedure in a fume
 hood.   PPE-splash
 proof    safety
 goggles,  lab coat,
 neoprene    or
 chlorinated
 polyethylene gloves

 PPE optional.  PPE-
 safety glasses, lab
coat, rubber gloves
optional.       CPE
adequate

-------
                                                              33-3
                                                   PPE and/or
Procedure              Potential Exposure     Engineering Controls

8. Analysis             None                   PPE optional.  PPE-
                                               lab    coat,    CPE
                                               adequate


WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S. EPA's Characterization  of Waste
From Standard Analytical Methods.
SPECIAL PRECAUTIONS

Follow biological  safety procedures  in  Chemical  Hygiene  Safety
Manual.  Wash hands thoroughly after procedure completion.

-------
                                                              34-1
                       CHEMICAL HYGIENE SOP
 Laboratory Procedure:

 Method:

 Description:


 Chemicals Used:
                    No.  34

               Analysis of phytoplankton

               Modified Utermohl

               Water samples are examined microscopically
               for identification and enumeration.

               Potassium iodide, iodine crystals,  nitric
               acid, hydrogen peroxide,  potassium
               dichromate,  hyrax,  acetic acid,  formal-
               dehyde
 Overall Glove  Recommendation:  Chlorinated Polyethylene (CPE)
 SAFETY CONTROLS
 Procedure
 1  .    L u
 solution,
 acetic
g o 1 ' s
 glacial
  acid,
Potential Exposure
 Inhalation
potassium   iodide,
iodine preparation

2.   Addition    of
Lugol's to sample
             Splashing
3 .    Sample
preservation  with
formaldehyde
            Spills, inhalation
     PPE and/or
Engineering Controls
  Do  procedure  in  a
  fume  hood.    PPE-
  splash proof safety
  goggles,  lab coat,
  CPE or butyl gloves

  General ventilation
  adequate.      PPE-
  safety glasses, lab
  coat, butyl gloves.
 All PPE is optional
 but recommended in
 event of spill

 Formaldehyde  is a
 carcinogen .
 Procedure  must be
 done    in    the
 Designated    Area
 fume  hood.   PPE-
 splash proof safety
 goggles, lab coat,
 viton   or   butyl
 gloves.         CPE
 adequate

-------
  Procedure

  4.    Analytical
  procedure ,
  preliminary
  screening
  5  .     Sample
  sedimentation,
  sedimented counting
 6. Diatom analysis,
 digestion    in
 centrifuge     with
 nitric acid
 7. Diatom analysis,
 addition     of
 hydrogen peroxide,
 potassium
 dichromate,  nitric
 acid
 Potential Exposure

  None
8. Diatom analysis,
centrifuge
 None
 Spills,  splashing,
 inhalation
Spills,  splashing,
inhalation
Inhalation
                 34-2
     PPE  and/or
Engineering. Controls

  PPE optional.  PPE-
  lab coat,  rubber or
  CPE   gloves   when
  handling organisms.
  Decontaminate   all
  surfaces,   wash
  hands

  PPE optional.  PPE-
  lab coat, rubber or
  CPE  gloves  when
  handling organisms.
  Decontaminate   all
  surfaces,    wash
  hands

  Nitric   acid    is
  incompatible  with
  heat   and   common
 metals.    Extreme
 care must be taken.
 Do  procedure  in a
 fume hood or local
 exhaust.       PPE-
 splash proof safety
 goggles,  lab coat,
 CPE,   viton    or
 rubber gloves, face
 shield optional

 Do procedure in a
 fume hood or local
 exhaust.  Hydrogen
 peroxide     and
 potassium
dichromate   are
oxidizers,    keep
from   flammables.
PPE-splash   proof
safety goggles, lab
coat, rubber or CPE
gloves

Use  local  exhaust
while centrifuging.
PPE-safety glasses,
lab coat,  rubber or
CPE gloves

-------
                                                              34-3

 Procedure             Potential Exposure     Engineering Controls
PPE and/or             M
 9.   Diatom  slide     Heat (burns)           Use    insulated
 preparation,                            gloves   or  tongs
 heating   on   hot                            handling hot items
 plate

 10.   Addition   of     None                   FPE-safety  glasses,
 hyrax    mounting                            lab coat, rubber or
 median                                         CPE gloves
 WASTE GENERATION AND HANDLING

 Follow all  guidelines in the U.S. EPA's Characterization of Waste
 From Standard Analytical Methods.


 SPECIAL PRECAUTIONS

 Follow all Biosafety Rules in the Chemical Hygiene Safety Manual.

 Preservation with formaldehyde must be done with extreme caution.

Wash hands thoroughly after procedure completion.

-------
                                                               35-1
 Laboratory  Procedure:

 Method:

 Description:

 Chemicals Used:
                        CHEMICAL HYGIENE SOP
        No.  35

   Analysis of zooplankton
   Zooplankton are examined microscopically.

   Formaldehyde, magnesium carbonate, sodium
   hypochlorite, polyvinyl lactophenol
 Overall Glove Recommendation:  Chlorinated Polyethylene (CPE)

 SAFETY CONTROLS
 Procedure

 1. Lugol's solution
 preparation

 2.  Analysis,  sub
 sample counts
 3.  Microcrustacean
 analysis
4  .     Sample
preservation   with
formaldehyde
Potential Exposure

 See SOP No. 34


 None
 None
Spills, inhalation
5.  Rotifer  sample
analysis
Biological
     PPE and/or
Engineering Controls

  See SOP No.  34
 PPE optional.  PPE-
 lab coat, rubber or
 CPE   gloves   when
 handling samples

 PPE optional.  PPE-
 lab coat, rubber or
 CPE   gloves   when
 handling samples

 Formaldehyde  is a
 carcinogen .
 Procedure must be
 done     in     the
 Designated    Area
 fume  hood.    PPE-
 splash proof safety
 goggles,  lab coat,
 viton, CPE or butyl
 gloves

 PPE optional.  PPE-
 lab coat, rubber or
 CPE   gloves   when
 handling  samples

-------
                                                             35-2
WASTE GENERATION AND HANDLING
Follow all guidelines in the U.S. EPA's Characterization  of Waste
From Standard Analytical Methods.


SPECIAL PRECAUTIONS

Follow  Biosafety Rules  in the  Chemical  Hygiene  Safety  Manual.
Formaldehyde must be handled with care.

Wash hands thoroughly after procedure completion.

-------
                                                               36-1
                        CHEMICAL HYGIENE SOP

                               No.  36

                          Analysis of total phosphorous

                          CRL MIN7315

                          Organic phosphorous is digested.  The
                          phosphate ions react to form a blue
                          complex which is determined spectro-
                          photometrically.

                          Sulfuric acid, ammonium persulfate,
                          Levor IV,  ammonium molybdate,  ascorbic
                          acid,  potassium antimony1 tartrate,
                          potassium dihydrogen phosphate,
                          adenosine-5'-monophosphoric acid,
                          hydrochloric  acid

 Overall  Glove Recommendation:   Chlorinated Polyethylene  (CPE)
Laboratory Procedure:

Method:

Description:
Chemicals Used:
 SAFETY CONTROLS
 Procedure

 1.   Sulfuric   acid
 solution,
 preservation     of
 samples
                      Potential  Exposure

                       Spills,  splashing,
                       inhalation
2.     Ammonium
persulfate solution
preparation
                      Spills,  splashing,
                      inhalation
     PPE and/or
Engineering Controls

  Do procedure  in a
  fume   hood.     Add
  acid  slowly, allow
  to  cool.     PPE-
  splash proof safety
  goggles,  lab coat,
  viton,  chlorinated
  polyethylene,
  polyethylene gloves

  This material  is a
  strong    oxidizer.
  Keep from  heat  and
  flammables.      Do
 procedure in a fume
 hood.   PPE-splash
 proof    safety
 goggles, lab coat,
 viton,   butyl    or
 neoprene   gloves.
 CPE adequate

-------
 Procedure

 3.    Working
 digestion solution,
 sulfuric    acid,
 solution, ammonium
 persulfate solution
 4.    Ammonium
 molybdate    stock
 solution
 5.  Ascorbic   acid
 stock solution
6.     Potassium
antimonyl tartrate
stock solution
          Potential Exposure

           Spills,  splashing,
           inhalation
          Spills,  splashing,
          inhalation
          Splash
          inhalation
            ing
          Spills,  inhalation
7.   Combined
reagent mix
color
Spills,  splashing,
inhalation
                36-2
     PPE and/or
Engineering Controls

  Keep  from heat and
  flammables.     Mix
  materials
  carefully.       Do
  procedure in a fume
  hood.    PPE-splash
  proof    safety
  goggles,    face
  shield,  lab  coat,
 viton gloves.   CPE
 adequate

 Do  procedure  in  a
 fume  hood.     PPE-
 splash proof safety
 goggles, lab  coat,
 rubber  gloves  (no
 data  on  gloves).
 CPE adequate

 Do procedure  in a
 fume  hood.    PPE-
 splash    proof
 goggles, lab coat,
 rubber gloves.  CPE
 adequate

 Do procedure  in a
 fume  hood.    PPE-
 splash proof safety
 goggles,  lab coat,
 rubber gloves  (no
 data  on  gloves).
 CPE  adequate

 Do procedure  in  a
 fume hood.   Follow
 instructions    for
 mixing    in    lab
 procedures.    PPE-
 splash proof safety
 goggles,  lab coat,
 rubber gloves.   CPE
 adequate

-------
  Procedure

  8.    Manifold
  dilution  water
  9  .     Stock
  phosphorous
  calibration
  standard, potassium
  dihydrogen
  phosphate, sulfuric
  acid

  10.    Stock
 phosphorous control
 standard,
 adenozine-5'-
 monophosphoric
 acid, sulfuric acid

 11. Digestion, wash
 glassware    with
 hydrochloric acid
 12.     Digestion,
 preparation    of
 calibration
 standards

 13.     Digestion,
 heating tubes
14.  Analysis
 Potential  Exposure

  Splashing
 Spills,  splashing,
 inhalation
 Spills, splashing,
 inhalation
 Spills,  splashing,
 inhalation
 Spills, inhalation
Inhalation,    heat
(burns)
Inhalation
                36-3
     PPE and/or
Engineering Controls

  Do procedure  in a
  fume  hood.    PPE-
  splash proof safety
  goggles,  lab coat,
  rubber gloves.  CPE
  adequate

  Do procedure in  a
  fume   hood.    PPE-
  splash proof safety
  goggles,  lab  coat,
  viton  gloves.  CPE
  adequate
 Do  procedure  in  a
 fume  hood.   PPE-
 splash proof safety
 goggles, lab coat,
 viton gloves.  CPE
 adequate

 May  cause  severe
 skin or respiratory
 burns.        Do
 procedure in a fume
 hood.   PPE-splash
 proof     safety
 goggles,  lab coat,
 CPE,   neoprene  or
 rubber gloves

 Use   local  exhaust
 over    operation.
 PPE-as outlined for
 each  standard

 Use  local  exhaust
 over    operation.
 Allow a clean air
 purge   before
 opening.       Use
 insulated    gloves
 handling hot items

Use  local  exhaust
over    operation.
PPE-as outlined for
each standard

-------
                                                              36-4

WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.


SPECIAL PRECAUTIONS

Use care preparing digestion solution to prevent violent reactions.

-------
                                                               37-1
  Laboratory Procedure:


  Method:

  Description:


  Chemicals  Used:
 CHEMICAL HYGIENE SOP

        No. 37

   Determination of calcium/ magnesium,
   potassium, and sodium.

   Flame atomic absorption.

   Water samples are analyzed by atomic
   absorption.

   Nitric acid,  hydrochloric acid, calcium
   standard, magnesium standard,  potassium
   standard, sodium standard
 Overall Glove Recommendation:  Chlorinated Polyethylene (CPE)

 SAFETY CONTROLS
 Procedure

 1 .     Sample
 preservation  with
 nitric acid
Potential Exposure

 Spills, splashing/
 inhalation
2 .     Calcium
standards  /
intermediate
standards/ working
standards in nitric
acid
Spills,  splashing,
inhalation
     PPE and/or
Engineering Controls

 Nitric   acid   is
 incompatible   with
 heat, common metals
 and    powde red
 metals.     Extreme
 care must be taken.
 Do  procedure in  a
 fume  hood.    PPE-
 splash proof safety
 goggles/  lab  coat/
 viton   or   rubber
 gloves, face shield
 optional.      CPE
 adequate

 Nitric   acid   is
 incompatible  with
 heat,  common metals
 and    powde red
 metals.    Extreme
 care must be taken.
 Do procedure  in  a
 fume  hood.    PPE-
 splash proof safety
 goggles,  lab coat,
 viton   or   rubber
 gloves, face shield
 optional.       CPE
 adequate

-------
 Procedure

 3.    Magnesium
 standard,
 intermediate
 standards, working
 standards,    in
 nitric acid
Potential Exposure

 Spills,  splashing,
 inhalation
 4.    Potassium
 standard,
 intermediate
 standards,  working
 standards in nitric
 acid
 Spills,  splashing,
 inhalation
5. Sodium standard,
intermediate
standard,  working
standards in  nitric
acid
Spills, splashing,
inhalation
                37-2
     PPE and/or
Engineering Controls

 Nitric   acid   is
 incompatible   with
 heat, common metals
 and    powdered
 metals.     Extreme
 care must be taken.
 Do  procedure in  a
 fume  hood.    PPE-
 splash proof safety
 goggles,  lab  coat,
 viton   or  rubber
 gloves, face shield
 optional.      CPE
 adequate

 Nitric   acid   is
 incompatible  with
 heat,  common metals
 and    powdered
 metals.
 Extreme care  must
 be  taken.      Do
 procedure in a fume
 hood.    PPE-splash
 proof    safety
 goggles,  lab coat,
 viton   or   rubber
 gloves, face shield
 optional.       CPE
 adequate

 Nitric   acid   is
 incompatible  with
 heat, common metals
 and    powdered
 metals.     Extreme
 care must be taken.
 Do procedure in  a
 fume  hood.    PPE-
 splash proof safety
 goggles,  lab coat,
 viton   or   rubber
 gloves, face shield
 optional.       CPE
 adequate

-------
 Procedure

 6 .    Instrument
 calibration, burner
 operation
Potential Exposure

 Heat     (burns) ,
 compressed    gas
 cylinder explosions
 7 .    Instrument
 calibration
 Inhalation, spills
 8.     Analytical
 procedure   washing
 with   hydrochloric
 acid
Spills, splashing,
inhalation
WASTE GENERATION AND HANDLING
                37-3
     PPE and/or
Engineering Controls

  Keep   combustibles
  away from operating
  area.     Acetylene
  tank must be secure
  and    upright.
  Follow  all  safety
  procedures in
  Compressed    Gas
  Association
  Pamphlet P-l

  Do    as    many
  procedures    as
  possible in a fume
  hood     or    use
  exhausting  during
  operation.    PPE-
  safety  glasses  or
  splash proof safety
 goggles, lab coat,
 rubber gloves.  CPE
 adequate

 May  cause  severe
 skin or respiratory
 burns.       Do
 procedure in a fume
 hood.    PPE-splash
 proof    safety
 goggles, lab coat,
 neoprene or rubber
 gloves.         CPE
 adequate
Follow all guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.
SPECIAL PRECAUTIONS

None

-------
                                                              38-1
 Laboratory Procedure:


 Method:

 Description:

 Chemicals  Used:
 CHEMICAL HYGIENE SOP

        No.  38

   Determination of total calcium,
   magnesium, potassium,  and sodium

   ICAP

   Water samples are analyzed by ICAP.

   Nitric acid,  plasma  grade standards:
   calcium,  magnesium,  potassium, sodium
 Overall Glove Recommendation:  Chlorinated Polyethylene (CPE)
 SAFETY CONTROLS
 Procedure

 1.   Nitric    acid
 handling,
 dilutions,   sample
 preservation
Potential Exposure

 Spills,  splashing,
 inhalation, heating
2.  Calcium
None
     PPE and/or
Engineering Controls

 Nitric    acid   is
 incompatible   with
 heat    and    most
 common  metals  and
 powdered    metals.
 Extreme  care  must
 be    used    when
 heating.         Do
 procedure in a fume
 hood.   PPE-splash
 proof     safety
 goggles,  lab  coat,
 viton gloves,  face
 shield.         CPE
 adequate

 Do  procedure  in  a
 fume  hood.    PPE-
 splash proof safety
 goggles, lab  coat,
 rubber glove  (data
 inconclusive    on
 gloves).        CPE
 adequate

-------
                                                             38-2
Procedure

3. Potassium
Potential Exposure

 Inhalation
4. Sodium
                       Inhalation
5. Magnesium
                       Inhalation
6.   AROO   standard
preparation    in
nitric  acid
 Spills,  splashing,
 inhalation, heating
     PPE and/or
Engineering Controls

  Do procedure  in  a
  fume   hood.    PPE-
  splash proof safety
  goggles,  lab coat,
  rubber gloves (data
  inconclusive    on
  gloves).        CPE
  adequate

  Do procedure  in  a
  fume   hood.    PPE-
  splash proof safety
  goggles,  lab coat,
  rubber gloves (data
  inconclusive    on
  gloves).        CPE
  adequate

  Do procedure  in  a
  fume   hood.    PPE-
  splash proof safety
  goggles,  lab coat,
  rubber gloves (data
  inconclusive    on
  gloves) .        CPE
  adequate

  Nitric   acid   is
  incompatible  with
  heat    and    most
  common metals  and
  powdered   metals.
  Extreme  care  must
  be    used    when
  heating.        Do
  procedure in a fume
  hood.    PPE-splash
  proof     safety
  goggles,  lab coat,
  rubber gloves  (acid
  is diluted).   CPE
  adequate

-------
 Procedure

 7.  AR01  standard
 preparation ,
 calcium, magnesium
 in nitric acid
Potential Exposure

 Spills,  splashing,
 inhalation, heating
 8.    Lab   control
 standards, high and
 low
 Inhalation
 splashing
9.    Intermediate
solution     and
dilution,
magnesium, calcium,
in nitric acid
Inhalation
splashing
                38-3
     PPE and/or
Engineering Controls

  Nitric    acid   is
  incompatible   with
  heat    and    most
  common  metals  and
  powdered    metals.
  Extreme  care  must
  be    used     when
  heating.         Do
  procedure in a fume
  hood.   PPE-splash
  proof     safety
  goggles, lab  coat,
  rubber gloves  (acid
  is diluted).   CPE
  adequate

 Nitric   acid    is
 incompatible  with
 heat    and    most
 common  metals and
 powdered   metals.
 Extreme care  must
 be    used    when
 heating.        Do
 procedure in a fume
 hood.    PPE-splash
 proof    safety
 goggles,  lab coat,
 rubber gloves (acid
 is diluted).   CPE
 adequate

 Nitric  acid    is
 incompatible   with
 heat    and    most
 common metals  and
 powdered    metals.
 Extreme  care  must
 be    used     when
 heating.         Do
 procedure in a fume
 hood.    PPE-splash
 proof    safety
 goggles, lab coat,
 rubber gloves (acid
 is  diluted) .    CPE
 adequate

-------
                                                              38-4
                                                   PPE and/or
 Procedure             Potential Exposure     Engineering Controls

 10.    Analytical     Inhalation             Do   as   much   as
 procedures ,                            practical  under  a
 calibrations,  etc.                             fume   hood  or   a
                                               local exhaust vent.
                                               Avoid    breathing
                                               fumes.   PPE-safety
                                               glasses  or  splash
                                               proof    safety
                                               goggles, lab coat,
                                               rubber gloves.  CPE
                                               adequate


 WASTE  GENERATION AND HANDLING

 Follow all guidelines  in the U.S. EPA's Characterization of Waste
 From Standard Analytical Methods.


 SPECIAL PRECAUTIONS

 Many standards used are carcinogenic  or very poisonous.   Extreme
 care must be taken when  handling these  materials.    Follow  all
 procedures carefully in the analytical procedure  manual.

Nitric acid  used  in these  procedures is  dilute  enough to  most
 likely not react with metals and heat.   However, care must still be
used.

-------
                                                               39-1
 Laboratory Procedure:


 Method:

 Description:

 Chemicals Used:
 CHEMICAL HYGIENE SOP

        No 39

   Liquid / liquid water extraction
   capture
   Not yet determined.

   Methylene chloride
 Overall Glove Recommendation:  Choice of below.
 SAFETY CONTROLS
 Procedure

 Use  of  methylene
 chloride
Potential Exposure

 Spills,  splashing,
 inhalation ,
 flammable
     PPE and/or
Engineering Controls

  Concentrated vapors
  can  be ignited  by
  high heat source or
  flame.  Material is
  a    suspected
  carcinogen .
  Procedure  must  be
  done    in    the
  Designated    Area
  fume  hood.    PPE-
  splash proof safety
  goggles, lab  coat,
  viton,   polyvinyl
  alcohol or  neoprene
  gloves.     These
  should    offer
  adequate protection
WASTE GENERATION AND HANDLING

Follow all guidelines in the U.S. EPA's Characterization of Waste
From Standard Analytical Methods.
SPECIAL PRECAUTIONS

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                                                               39-1
                        CHEMICAL HYGIENE SOP

                              NO.  39
 Laboratory Procedure;


 Method:

 Description:
         Liquid / Liquid Water Extraction
         Capture

         Extraction

         Particulate free water is passed
         through an agitated chamber containing
         dichloromethane (DCM).  The DCM settles
         from  effluent  water back into the
         mixing chamber and the extracted water
         flows to waste.
Chemicals  Used:
        Methylene Chloride  (DCM)
        resi-analyzed methanol
        3,5 dichlorobiphenyl
        2,3,5,6 tetrachlorobiphenyl
        2,3,4,4',5,6 hexachlorobipheny1
SAFETY CONTROLS
Procedure

Use of methylene
chloride
Potential Exposure

 Spills, splashing,
 inhalation, flammable
       PPE and/or
Engineering Controls

 Concentrated vapors
 can be ignited by
 high heat source or
 flame.  Material is
 a suspected carcin-
 ogen.   Procedure
 must be done in the
 designated area
 fume hood.   PPE -
 splash proof safety
 goggles,  lab coat,
 viton,  polyvinyl
 alcohol or neoprene
 gloves.   These
 should offer
 adequate protection

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                                                          39-2


 Use of methanol      Spills,splashing,         Methanol  is  flam-
                      flammable, inhal-         mable.  Keep away
                      lation                    from ignition
                                                sources.  Perform
                                                procedure in fume
                                                hood.  PPE -  splash
                                                proof goggles, lab
                                                coat, rubber  or ne-
                                                oprene gloves.


 Use of PCB            Spills,  inhal-           Suspected
 standards             lation,  skin,             Carcinogen.  Avoid
                       eye contact.              breathing vapor
                                                or mist.   PPE-
                                                lab coat,  splash
                                                goggles,  neoprene
                                                gloves  suggested.
                                                Perform in area
                                                fume  hood.
 WASTE  GENERATION AND HANDLING

 Reagents  containing methylene  chloride must  be disposed  of as
 hazardous  waste.    Follow  all  guidelines  in  the  U.S.  EPA's
 Characterization of Waste From Standard Analytical Methods.

 Disposal of PCBs is strictly regulated by the federal government.
 All waste residues containing PCBs  (e.g. wiping cloths, absorbent
 material, used disposable protective gloves,  clothing, etc.) should
 be collected, placed in proper containers, marked and disposed of
 in the manner prescribed by EPA  regulations  (See 40 CFR Part 761).
 Consult these regulations prior to any disposal of PCBs.
SPECIAL PRECAUTIONS

Keep containers closed when not in use.

Wash hands thoroughly after procedure completion.

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                                                               40-1
                        CHEMICAL HYGIENE SOP

                              NO. 40
 Laboratory  Procedure:

 Method:

 Description:
         Sampling for PCB Congeners

         Filtration

         Water is pumped to deck of research
         vessel through a hose.   Water  is  then
         carried through a filtration system to
         remove the particulate  phase.  The
         filtrate is stored for later processing
         and analysis.
 Chemicals Used:
        Acetone
 SAFETY CONTROLS
 Procedure

 Wash carboys  and
 parts in Acetone
Potential Exposure

 Spills,  Splashing,
 Inhalation,  Fire
WASTE GENERATION AND HANDLING
         PPE  and/or
  Engineering Controls

 Acetone  is Flammable.
 Do procedure in a
 fume hood when
 possible.  Keep from
 ignition sources.  PPE
 Splash proof goggles,
 lab coat, rubber or
 neoprene gloves.
 Check work area with
properly calibrated
LEL meter when
cleaning carboys
outside fume hood.
Stop if greater than
10% LEL for Acetone.
Reagents containing acetone must be disposed of as hazardous waste.
Follow all guidelines in the U.S.  EPA's Characterization of Waste
From Standard Analytical Methods.

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                                                        40-2
SPECIAL PRECAUTIONS

Use care in handling f lanunables.  Keep containers closed when not
in use.

Wash hands thoroughly after procedure completion.

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                                                    SECTION: APPENDIX M
                                                    VERSION: FINAL/MAY 1997
                                                    PAGE: 1  of 40
APPENDIX M:  GLNPO OCCUPANT EMERGENCY PLAN/FIRE PREVENTION PLAN

TABLE OF CONTENTS

CHAPTER 1 - GENERAL

       1.  General
       2.  Authority
       3.  Applicability
       4.  Annual Review

CHAPTER 2 - ORGANIZATION

       1.  Responsibilities
       2.  Advisory Committee
       3.  Coordinator
       4.  Occupants
       5.  Basic Functions
       6.  Succession to Command
       7.  Vessel/Building Sheet

CHAPTER 3 - DUTIES AND RESPONSIBILITIES

       1.  Designated Official's Duties
       2.  Deputy Designed Official's Duties
       3.  Occupant Emergency Coordinator
       4.  Area Coordinators
       5.  Zone Monitor
       6.  Stairway/Elevator Monitor
       7.  Medical Officer
       8.  First-Aid Monitor
       9.  Fire Marshal
       10. Fire Protection Monitors
       11. Utilities Officer
       12. Utilities Control Team
       13. Bomb Search & Reconnaissance Officer
       14. Bomb Search Team
       15. Supply Officer
       16. Supply Assistants
       17. Welfare Officer
       18. Welfare Assistant

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                                                    SECTION:  APPENDIX M
                                                    VERSION:  FINAL/MAY 1997
                                                    PAGE:  2 of 40
CHAPTER 4 - TRAINING

       1.  General
       2.  Prior Experience
       3.  Civil Defense Training for Federal Employees
       4.  Education Program
       5.  Drills

CHAPTER 5 - WARNING AND ALARM SYSTEMS

       1.  Civil Defense Warning Signals
       2.  Disaster Alarm  Signals
       3.  Communications

CHAPTER 6 - EMERGENCY AND EVACUATION PROCEDURES

       1.  Control Center
       2.  Emergency Procedures
       3.  Evacuation Procedures

A. Fire in the Facility
B. Bomb Threats and Civil Disorder
C. Instruction Sheet for Initial Bomb Threat Report
D. Bomb Threat Data Report
E. Instruction Sheet for Follow-up Report
F. Demonstrations
G. Explosions
H. Chemical Accidents
I.  Severe Windstorms
J.  Earthquakes
K. Enemy Attack
L. Panic
M. Physical Security of Facilities Alert Guidelines
N. Emergency Procedures  [includes Fire Prevention (29 CFR 1910.38)]
O. Occupant Emergency Personnel
P. Protection Plan Personnel
Q. Personnel to be Contacted in Event  of Emergency

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                                                           SECTION: APPENDIX M
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE:  3 of 40
CHAPTER 1:  GENERAL

1.   General.  The Great Lakes National Program Office has an inherent responsibility to minimize the
    danger to life and property arising from the effects of bomb threats, bombs, enemy  attack,  fire,
    explosion,  earthquake,  serious  weather  disturbances,  civil  disturbances,  and  other  disasters
    affecting the R/V  Lake  Guardian.    To cope  with these  conditions, GLNPO requires   the
    development of an occupant emergency  plan and fire prevention plan in accordance with 29 CFR
    1910.38. This plan is effective  upon receipt and for execution upon direction  of the Designated
    Official and/or Director of Facilities. The Occupant Emergency Plan for GLNPO occupants of
    the Chicago, Illinois General Services Administration Federal Building located at 77 West Jackson
    Blvd. is maintained and  administered by  the Regional Safety Office and is not included  in  the
    GLNPO Health, Safety & Environmental Compliance Manual.

2.   Authority.  The Great Lakes National Program Office has the authority to protect life and property
    in their owned and  occupied buildings or  vessels, and is  responsible  for the development of
    emergency  preparedness and prevention programs.

3.   Applicability.  The responsibilities and  procedures in  this  plan apply to all property under  the
    charge and  control of the Great  Lakes National Program  Office and  to all persons entering in or
    on such property.  Each occupant shall  be  responsible for the observance of these rules  and
    regulations.

    This  plan will be  made known to all property occupants by:

    •  Posting alarm signals on bulletin boards.

    •  Posting evacuation routes on bulletin boards.

    •  Posting a listing of protection  personnel on bulletin boards.

    •  Distributing emergency instructions to all personnel and contractors.

4.   Annual Review.

    a.        It is the responsibility of all Facility Occupant Emergency Officials to keep the roster of
             personnel current and to perform, in conjunction with all protection personnel, an annual
             review of the plan.

    b.        During  this annual  review, any recommended changes, additions, and deletions will be
             submitted.  However, pertinent changes should be submitted as they occur.

    c.        The Designated Official  will transmit the required  changes to the plan to all protection
             officials.

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    d.        Contractors are  responsible  for  printing  and  distributing  copies of  the Occupant
             Emergency Plan to their personnel.

CHAPTER 2: ORGANIZATION

1.  Responsibilities

    a.        The establishment of the Protection Organization  is a joint responsibility of all personnel
             and  contractors.   The R/V Lake Guardian ship operating contractor,  the  full-time
             occupants of R/V Lake Guardian, and the  contract Chemical Hygiene Officer (CHO),
             will  be responsible for providing the direction for  implementation of the Occupant
             Emergency Plan.  Personnel for the Organization will  be recruited on an equitable basis
             from among all ship-board occupants prior to each cruise.
    b.        To  the extent  possible and as conditions  permit,  GLNPO  will  install and  maintain
             protective  equipment,  such  as  warning  devices,  alarm  systems,  and  fire-fighting
             apparatus and will provide employee training for the  operation of the equipment.

2.   Advisory Committee. An advisory committee will be established to assist in the development of
    various phases of the Plan.  The committee will  consist of a representative from each agency who
    is assigned the responsibility for the development of the plan.

3.   Coordinator.  An official of GLNPO  or the ship operating contractor will be the Coordinator for
    GLNPO  vessels.   The  Coordinator  is responsible   for providing  leadership, assistance, and
    follow-up to  ensure  establishment, implementation, and continuity of the Plan.  He will provide
    information and guidance, advise on establishing the Organization, and will assist the Designated
    Official  in recruiting qualified personnel  for  technical services such as  utilities control and for
    making arrangements for training organization  personnel.

4.   Occupants.   Occupants  will  acquaint themselves   with  the  alarm  system  and  emergency
    instructions.  All occupants are expected to react to alarms promptly and follow the directions of
    Organization  personnel during an emergency.

5.   Basic Functions.  Personnel will perform the following functions:

    a.        Evacuation  of Occupants.    This  function provides  for  the planned  and   directed
             evacuation  of all  occupants  in the event of an emergency.  This is  performed under the
             direction of the Occupant Emergency Coordinator assisted by the Area Coordinators and
             their assistants.

    b.        Fire Protection.  This function provides for the fighting  and control  of fires (small fires)
             before the  arrival of local  fire-fighting  forces.   This  is  performed  by fire protection
             personnel designated by the  R/V Lake Guardian Station  Bill.

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    c.        First Aid. This function provides for emergency medical treatment by personnel trained
             in administering first aid.  It is performed by the Medical Officer (usually the Captain or
             Chemical Hygiene Officer).

    d.        Utilities  Control.   This function provides  for  control of utilities  in the  event of  an
             emergency.  It includes the control  of all  electrical and mechanical  equipment; water,
             gas,  and  steam  valves  and conduits; power  switches,  ventilation  and  refrigeration
             devices;  and  any  other  such  equipment  in  the facility.    Personnel employed  in
             maintenance and  facility operations will be assigned to this function under the  direction
             of the Utilities Officer (Chief Engineer).

    e.        Bomb  Search  and Reconnaissance.  This function provides  for the  handling  of bomb
             threats, bomb searches, and reconnaissance.

6.   Succession to  Command.   To provide continuity, certain  persons  are  designated  to command
    should the Designated  Official  be unable  to  discharge the duties and responsibilities of that
    position.  The  succession to command is as follows (descriptions on page  8):

    a.  Alternate Designated Official - Captain

    b.  Deputy Designated Official -  1st Mate

    c.  Alternate Deputy Designated Official -  Chemical Hygiene Officer

    d.  Occupant Emergency Coordinator - Captain

    e.  Alternate Occupant Emergency Coordinator - Chemical Hygiene Officer

    f.  Fire Marshal - Captain

    g.  Alternate Fire Marshal - 1st Mate

    h.  Bomb Search and Reconnaissance Officer - Outside Assistance

    i.  Alternate Bomb Search and Reconnaissance Officer - Outside Assistance

    j.  Utilities Officer - Chief  Engineer

    k.  Alternate Utilities Officer - Assistant Engineer

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7.0 Vessel/Building Sheet
                                                        SECTION:  APPENDIX M
                                                        VERSION:  FINAL/MAY 1997
                                                        PAGE:  6 of 40
Location:    24 Sixth Street
            Bay City Michigan, 48708

Name:  U.S. EPA, GLNPO R/V Lake Guardian

Construction:
Length
Beam
Draft
Berthing Capacity
Cruising Speed
Fuel Consumption
180'
40'
11' - 15'
42
12 Kts.
110 GPH
Number of Occupants: 42 maximum

a.  Area Coordinators

b.  Zone Monitors

c.  Ground Floor Exit Monitors

d.  First-Aid Monitors

e.  Fire Protection Monitors
Range
Endurance
2 Main Engines
2 Propellers
3 Generators
2 Radars
6000 miles
15-30 days
1150 HP
4 blade stainless
135 KW
40 Mile capability

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                                                            SECTION:  APPENDIX M
                                                            VERSION: FINAL/MAY 1997
                                                            PAGE:  7 of 40
CHAPTER 3:  DUTIES AND RESPONSIBILITIES

1.    Designated Official's Duties. The Designated Official is responsible for:
    •   developing the  Plan  to protect life and property and to minimize damage in the event of a
        civil  defense emergency or other disaster
    •   coordinating such  planning with  the Director of Facilities,  tenant agency officials, and the
        local civil defense  director
        selecting,  organizing, and  training an adequate  staff to conduct the emergency operations
        required by the  facility's Plan
        directing and supervising the activities of the occupants during  an enforced stay within the
        facility. The Designated Official  performs the following dudes:

        a.    Ensures that  the basic provisions of the Plan are disseminated to all persons employed
              in the facility.

        b.    Requests  the Director of Facilities  to assist in planning for,  obtaining, and installing
              necessary alarm systems, and in selecting qualified emergency service personnel.

        c.    Establishes and reports to a control  point from which the activities of the Organization
              may be directed in an emergency.

        d.    Maintains liaison  and cooperates with the principal officers of the tenant agencies,  or
              their designees, on problems  arising in the selection  and training of tenant agency
              employees for the Organization.

        e.    Arranges  for  posting,  on   appropriate  bulletin  boards, a  roster  of   Organization
              personnel  who  have responsibility for in-shelter or other emergency operations.

        f.    Takes  all  necessary actions  to ensure that  the facility's Organization   operates safely
              and  efficiently  in emergencies.

        g.    Exercises   command   responsibility  for  the  orderly  movement   of   all    personnel
              (including non-tenants) in accordance with the  Plan.

        h.    Maintains liaison  with the local  civil  defense  director  and local  fire  and  police
              departments.

        i.    Ensures that  appropriate training courses are provided and that arrangements are  made
              for obtaining assistance from the Red Cross, local fire and police departments and other
              sources, as required, to train the Organization staff.

        j.    With the  assistance  of the  Director of Facilities, coordinates arrangements  for. and
              directs, fire and bomb evacuation drills.

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        k.    Arranges  for and supervises  the  storage of required supplies  and equipment in the
              facility.

        1.     Conducts  periodic inspections  in the facility.

        m.   Directs all shelter management operations when fallout shelters are occupied.

        n.    Works closely and cooperates  with the local or state civil defense director in all matters
              involving  the facility's Plan, organization or operations.

2    Deputy Designated Official's Duties.  The Deputy  Designated Official will work closely with the
    Designated Official  and will assume all  duties of  the Designated Official  in  the  Designated
    Official's absence or if the Designated Official is incapacitated for any reason.

3.   Occupant  Emergency Coordinator.   The  Occupant  Emergency Coordinator  is  responsible for
    supervising  and  expediting  the  planned  and  controlled  movement  of  all  occupants  in  an
    emergency.   Under  the general direction of the Designated Official, the Coordinator serves as
    head of the Personnel Movement Service (Area Coordinators, etc.) and performs the follow duties:

        a.    Plans personnel  movement routes and establishes movement procedures to  effect the
              personnel  movement provisions of the Plan.

        b.    Assures that all Organization personnel responsible for occupant evacuation conduct an
              evacuation route  search for suspicious  items or  hazardous  conditions  BEFORE  any
              occupants are permitted to  pass through public areas.

        c.    Trains, and designates in writing, all Area Coordinators.

        d.    Develops  standard forms  of facility protection  assignments  to  be  used by Area
              Coordinators.

        e.    Supervises and directs movement of personnel during drills and actual emergencies in
              accordance with the Occupant  Emergency Plan.

        f.     Assures that all  occupants,  including  members  of  the Organization,  comply  with
              procedures indicated by the alarm signals as specified  by the Plan.

        g.    Reports to the control center during actual emergencies.

4.   Area Coordinators.   The Area Coordinators are  assigned  by and  under  the direction  of the
    Occupant Emergency Coordinator.  The Area Coordinators  act as a  liaison  and focal contact point
    between the Zone Monitors and  the Occupant Emergency Coordinator.  Area Coordinators assume
    supervision of all protection  personnel in the area and expedite  the evacuation of personnel from
    their assigned area.   Duties include the following:

    a.    Establishing contact with all Zone Monitors within their area of responsibility.

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   b.   During  drills or actual  emergencies, maintaining a clear  line of communication between the
        Occupant Emergency Coordinator and  Zone Monitors.

   c.   Maintaining a current roster of all self-protection personnel.

   d.   Assuring  that evacuation routes  are clearly identified, posted on  bulletin boards,  and well
        known to the regular occupants of the  area.

   e.   Assuring that evacuation procedures are well known to all regular occupants of the area.

   f.   Directing the  calm and orderly flow of personnel  during drills  or actual emergencies along
        the prescribed evacuation routes.

   g.   Immediately establishing an alternate route of evacuation  if a bomb, suspicious item or other
        hazardous condition is discovered along the  normal  route of evacuation.  No part of a straight
        line route will  be used  if a  suspicious  item  or other hazard  is  found  within a corridor.
        stairwell,  passageway, wing, etc.

   h.   Assuring  that Fire Protection Monitors know the location of their duty stations, location of
        fire-fighting equipment in their area of responsibility, and the alarm signals which will direct
        them to their stations.

   i.   Coordinating the activities of  Zone Monitors,  Stairway Monitors,  Fire  Protection Monitors,
        and First-Aid  Monitors.

   j.   Maintaining  a current  roster  of personnel  with evacuation assignments by  listing  name,
        emergency assignment,  telephone  number,  and duly  informing the Occupant Emergency
        Coordinator of any changes to  the roster.   The roster  form is provided by  the  Occupant
        Emergency Coordinator.

   k.   Immediately  reporting fires in his/her area  to  the  Control Center and Occupant Emergency
        Coordinator.

   1.   Reporting to the Control Center after evacuation has been completed.

5.  Zone Monitor.  Under  the direction  of the Area Coordinator, the Zone  Monitor supervises and
   expedites the evacuation of personnel  from  his assigned section of the  area.  Duties include:

   a.   Assuring  that  evacuation routes are  clearly identified and  are  well known to the regular
        occupants.

   b.   Directing the calm and orderly flow  of personnel  during drills  or actual emergencies along
        prescribed evacuation routes.

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    c.    Immediately establishing an alternate route of evacuation if a bomb, suspicious item or other
         hazardous condition is discovered along the normal route of evacuation.

    d.    Appointing a column leader to guide personnel along prescribed evacuation routes to an area
         specified by the Area Coordinator.

    e.    Assuring that all personnel  have vacated their areas when required.

    f.    Assuring that rooms are  vacated, windows and  doors  closed,  and electrical  appliances
         switched off in appropriate  areas during fire evacuations.

    g.    Assuring that windows and  doors  remain open and lights remain on  during bomb threat
         evacuations.
6.   Stairway/Elevator Monitor.   Under the direction of the Area Coordinator, the Stairway/Elevator
    Monitor controls the movement of personnel  in the stairway.  Duties include:

    a.   Keeping the stairwell open.

    b.   Keeping personnel moving calmly and  steadily, at a walking pace, and  not permitting them
        to run.

    c.   Closing doors after  all  personnel in line have passed through and  leaving the  post only after
        the last person  in line has passed through the door.

    d.   Controlling and/or restricting the use of  passenger elevators by personnel.
7.   Medical Officer.  The Medical Officer serves as head of the Health and First-Aid  Service.  The
    officer is  responsible for training  and equipping all  personnel  assigned to perform medical  or
    first-aid services in  an  emergency  and supervising  emergency  first-aid  or  medical  self-help
    operations within the facility during an emergency.  Duties include:

    a.   Arranging with the  American  Red Cross or other sources for first-aid and medical  self-help
        training for all Organization  personnel who require such training.

    b.   Supervising the selection of first-aid or medical treatment areas in  shelters and elsewhere  as
        required.

    c.   Directing first-aid or medical self-help operations and controlling access to medical  supplies.
        as required, to assure their proper use, conservation, and availability for emergency use.

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    d.    Establishing policies and rules governing the emergency treatment of ill and injured persons,
         maintaining adequate sanitation and  hygienic standards, and all  other  matters relating to
         emergency  health,  hygiene  and  medical  activities  during  an emergency  or  during the
         occupation  of an emergency shelter.

    e.    Inspecting the storage and handling of food and drinking water in emergency shelters.

8.   First-Aid Monitor.  Under the direction of the Medical Officer, the First-Aid Monitor administers
    immediate first-aid to injured personnel.  Duties include:

    a.    Knowing the location of medical supplies and treatment areas.

    b.    Administering first-aid to injured persons.

    c.    Knowing the extent  and  type of first-aid to be  administered in an emergency  situation and
         whom to notify if more treatment is needed.

    d.    Reporting to the Emergency Treatment Center to assist the Medical Officer in the absence of
         injured persons  and after  all occupants have been evacuated.

9.   Fire  Marshal. The  Fire Marshal organizes  fire-fighting teams for initial fire-fighting operations.
    Duties include:

    a.    Providing fire-fighting instructions through available sources.

    b.    Assuring that fire  fighters  know their  stations, locations of fire-fighting  equipment in  their
         area of responsibility, and the alarm signals which direct them to their duty stations.

    c.    Deploying  fire-fighting  personnel  to  fire areas  to extinguish or contain fires  pending the
         arrival of other  fire-fighting forces and cooperating with such forces as directed.

10. Fire Protection  Monitors. Fire  Protection  Monitors may  operate hand-type fire extinguishers if
    provided with yearly training.   Duties include:

    a.    Knowing the types of fires and appropriate extinguishers to use in fighting them.

    b.    Being familiar with the  location, operation and capacity of fire extinguishers in an assigned
         area.

    c.    Recognizing alarm signals, and then taking appropriate action.

    d.    Using care  in the operation of fire extinguishers to avoid unnecessary  damage  to personnel,
         equipment and records.

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11. Utilities Officer.  The Utilities Officer is responsible for controlling facilities and vessel utilities
   during  an emergency.   The Utilities Officer serves as head of the Utilities Control Team and
   performs the following duties:

   a.   Establishes a plan to attend to mechanical devices; ventilation, water, gas and steam valves;
        power switches, etc. during an emergency.

   b.   Dispatches individuals or  teams, at the sound of emergency alarms, to predetermined control
        points for preplanned or directed action.

   c.   Reports to the Control Center during emergencies.

12. Utilities Control Team.  Under the direction of the Utilities Officer, the Utilities Control Team is
   responsible for controlling utilities in a facility or on board a vessel during  an emergency.  The
   Utilities Control Team reports  to the Utilities Officer  during emergencies.

13. Bomb  Search  and Reconnaissance  Officer.   The Bomb  Search  and  Reconnaissance Officer  is
   responsible for recruiting, organizing, training and equipping personnel assigned to  bomb search
   activities.   He shall establish contact with bomb disposal units of local law enforcement agencies
   and with the U.S. Army  Explosive Ordnance Disposal Detachment in his area.  The duties and
   responsibilities of the Bomb Search and Reconnaissance Officer are as follows:

   a.   Ascertaining the structural composition  of the  facility or vessel  to  determine the possible
        effects of explosion.

   b.   Establishing a cascade telephone system to alert  all team  members  begin  implementation of a
        building or vessel search.

   c.   Coordinating search activities with local law enforcement search teams and/or the U.S. Army
        Explosive Ordnance Disposal Detachment in his  area.

   d.   Before personnel  are evacuated, validating  the  probability that a suspicious item  is most
        likely a bomb, time permitting.

14. Bomb  Search  Team.     Members   function   under   the  direction of the  Bomb  Search  and
   Reconnaissance Officer.   However,  they work directly  with  the Area Coordinator in their
   respective areas.  Members are generally selected from those positions within  the responsibility  of
   the Occupant Emergency Coordinator.  Duties include:

   a.   Searching  assigned areas  for bombs, suspicious packages  and items that do  not fit their
        surroundings.

   b.   Immediately reporting any suspicious items to the Area Coordinator who, in turn,  relays the
        information to the Bomb  Search and Reconnaissance Officer.

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    c.   Reporting to the Area Coordinator when the search has been completed.

15.  Supply Officer.  The  Supply Officer is responsible  for the care of, and  issuance to appropriate
    officials, all supplies, especially essential stocks of food, water, medical supplies, and  sanitation
    equipment.  He/she serves as head of the Supply  Service and  performs the following functions
    associated with  emergency shelter management.  Duties include:

    a.   Planning  and  scheduling  distribution  of  supplies to  appropriate  leaders and  regulates
        consumption in conformance with established emergency shelter management policy.

    b.   Procuring,  as available  and to the extent storage  space permits, desirable items  over and
        above basic stocking, through local government or other appropriate sources.

    c.   Establishing a  program regulating  the  acceptance  and  storage  of  issued  supplies and
        personnel  supplies;  maintaining an  up-to-date  inventory  of  all supplies  and equipment;
        providing for and maintaining security storage  of such material, and issuance of daily rations.
        medicines and other items to appropriate Organization    officials.

    d.   Training Supply Assistants in sufficient numbers  to expedite the supply functions of the Plan.

16.  Supply Assistants.   The  Supply Assistants assist the Supply Officer in the performance of his
    duties.

17.  Welfare Officer.  The  Welfare  Officer is responsible for the organization and  training  of a staff
    who will provide for registration of emergency shelter occupants and their subsequent needs, such
    as unit feeding, billeting, recreation, information and religious requirements.

18.  Welfare Assistants.   The  Welfare  Assistants assist the  Welfare Officer  in  meeting the feeding,
    billeting, recreation, information and religious requirements of emergency shelter occupants.
CHAPTER 4: TRAINING
    General.   A continuing  program  of training and  education  for  all  Organization  personnel is
    imperative  to  ensure  maximum effectiveness of the Organization.   Personnel  who  are assigned
    duties and  responsibilities in the Organization will  require and be given  specific  training in the
    skills necessary to perform their assigned  functions,  e.g.,  first-aid,  fire fighting,  etc.   The
    Designated  Official,  Director of  Facilities,  Fire  Marshal, and each  Area Coordinator,  will  be
    responsible for planning and arranging training (including periodic refresher training) of personnel
    appointed to the  Organization.   Such training  must be  sufficiently  extensive to  develop  and
    maintain proficiency  on  the  part  of each person  in order  that all emergency  duties  may  be
    accomplished rapidly  and  effectively.

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2.  Prior Experience.  In order to minimize training requirements, whenever possible, personnel will
be appointed to the Emergency Organization on the basis of prior experience.   Personnel  who  have
previously  served in similar organizations will be utilized to the  fullest extent possible.  In addition,
personnel will be considered for appointment on the basis of qualifications deriving from their normal
occupations (i.e., medical personnel, building maintenance, etc.).

3. Civil Defense Training for Federal Employees.

    a.    Executive Order No. 1052 of April 22, 1954  (19 F.R. 2379; 3 CFR)  provides that heads of
         Federal  agencies may authorize  personnel of their  respective agencies to participate in  civil
         defense  training  programs  and test  exercises  in  cooperation  with  any state  or political
         subdivision.   Federal employees may be released for periods of up to 40  working hours
         during  any calendar year for the purpose of receiving such civil defense training  under the
         provision of the Executive Order.

    b.    Each tenant  agency  is responsible for assuring  that those of its employees who have been
         assigned duties within the Organization are available for appropriate training.

4.  Educational  Program.   The Designated Official will  assure that all personnel are kept informed
    concerning the general nature  and concepts of the Plan and specific actions which all individuals
    are  required to take under the Plan.   Information  furnished  to the employees will be  clear and
    concise.  Video may be used periodically to advise all employees concerning  necessary individual
    survival preparation and actions.

5-  Drills.   Organization personnel and facility or vessel occupants will participate in drills that  will
    familiarize  them  with the actions they are  expected  to follow  in  the case of an  emergency.
    Evacuation drills will be conducted periodically under the direction of the Designated Official and
    Director of Facilities.  Drills will be announced in advance.  After they are conducted,  they should
    be documented in the ship's log.
CHAPTER 5: WARNING AND ALARM SYSTEMS
     Civil Defense Warning Signals.   The  local government is responsible for disseminating civil
    defense warnings to the public.  This is achieved through a system of centrally-controlled outdoor
    sirens.  There are two civil defense warning signals:

    a.   ALERT Signal.  This is a steadv tone or blast lasting three to five minutes which signals the
        broadcast of essential emergency information.   Occupants  will receive further  instructions
        from the public address system.

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    b.   ATTACK  Warning Signal.  This  is a wavering tone or series of short blasts lasting three to
        five minutes signaling  that an attack against the  country  has been detected.  Upon hearing
        this warning, personnel should take the best shelter available and keep away from windows.
        Upon orders from the Area Coordinator, all occupants will proceed to the shelter area.

    c.   Signals within  the Facility.  A complete warning system has been devised for the RV Lake
        Guardian  to operate manually by means of the General  Alarm  Switch,  the Fire Alarm
        Switch, and the Public Address system  located on the ship's bridge.   Also,  See the Station
        Bill in Chapter 8, Attachment 8.1.

2.  Disaster Alarm Signals. A non-civil defense disaster will be  signaled within the facility.
    a.   Fire Alarm.  This is  a  fire alarm  signal and a public address system announcement which
    informs occupants of the fire's location and instructs employees either to relocate or to complete
    the  facility's evacuation.   If the facility is evacuated, individuals should clear the  immediate area
    around the facility and  wait for verbal instructions from Organization officials.

    b.   Other Alarms.   A  rapid  ringing of the ship's bell  and a continuous ringing of general  alarm
    bells for a period of about ten seconds (in accordance with the  ship's  station bill), followed  by an
    oral alarm will  be used to warn personnel  of natural or other disasters.  Natural disasters include
    earthquakes, floods  and windstorms.   Other disasters  include explosions, escape  of lethal  gases,
    civil disturbances and bomb  threats.

3.    Communications Requirements.  In collaboration with the  Director of Facilities, the Designated
    Official will arrange  for communication  of warnings within the facility  or vessel.   Specific
    provisions will  be made for  informing  all occupants of the existence of different warning signals,
    their meanings  and  what  actions are  to be taken.   Warning signals  will be tested on a regular
    schedule to  ensure  that the equipment is working  properly.  Drills should be  scheduled often
    enough to enable the occupants to instantly recognize the  sound and meaning of all warnings and
    to automatically take appropriate action.
CHAPTER 6:  EMERGENCY AND EVACUATION PROCEDURES
1. Control Center. The Control Center on the R/V Lake Guardian is located on the 	
	Bridge  and is designated as the location that  will  be utilized  by the
Organization.  All Organization  personnel  must know  the  telephone  number  and location of the
Control Center in order for communications  to be established in an emergency.  During any period of
emergency shelter occupancy, either prior to enemy attack or natural disaster, or subsequent to attack
due to radioactive fallout, the Control Center may be shifted to O-2 Level.

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Emergency Procedures.

    a.   Upon notification by the  Designated Official, the Organization will iniorm the occupants of
        the emergency.  Emergency actions are stated at the end of Chapter 6 of this Appendix.

    b.   It is important that the emergency procedures be known in advance so immediate action  can
        be taken.  Delays caused by reviewing instructions after the alarm has sounded could  cause
        injury or death, which might be avoided if the occupants  were familiar with instructions in
        advance.

3. Evacuation Procedures.

    a.   Under no circumstances should an evacuation  take place due to a bomb  threat without  the
        evacuation route  first being searched for bombs or suspicious items.

    b.   Evacuation  may  be  required because  of an  emergency.   Normally, the Designated Official
        will decide whether one  area, various  areas,  or all  areas will  be evacuated.  The  Area
        Coordinator may decide to evacuate the area  if there is an emergency and he/she thinks there
        is insufficient time to counsel the Designated Official.  In  some cases, the Area Coordinator
        will be forewarned and will instruct the occupants to line up in the corridor at the stairways.

    c.   The Designated Official will inform each Area Coordinator of the existence of an emergency,
        its location, sequence of evacuation, and if the evacuation will be partial or complete.

        1.    A  partial evacuation will involve only  the  necessary area(s) and will be accomplished
              at the direction of the Area Coordinator.

        2.    A  complete evacuation involves  all areas.  If there is an emergency in  an area which
              requires  a  complete  evacuation, the  area  in  which the  emergency exists  will  be
              evacuated immediately, i.e., the disaster floor or deck  would be evacuated  immediately,
              followed by other areas in accordance with the ship's Station Bill.

A - Fire In The Facility

1.  Major Fire.  A major fire  is one which CANNOT be easily extinguished by the use  of a portable
    fire extinguisher.

2.  Minor  Fire.  A minor fire is one which  CAN  be  extinguished by  the use of a  portable fire
    extinguisher.

3.  When  a major fire  is  discovered, the  employee will  activate the nearest  fire  alarm  box and may
    call 911  on any  of the  ship's  telephones  to report  the fire.   Supervisors  will ensure that  all
    employees know the  locations  of alarm boxes and  telephone  systems in their area.  The  alarm
    boxes  and 911 emergency systems are  tied into the Bridge.

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4.   After an employee activates the fire alarm,  the Area Coordinator should be contacted and given
    the location of the fire.

5.   The Area Coordinator will contact the  Designated Official  and the  Fire Marshall, who will then
    decide  whether  evacuation  will  be partial  or  complete.   The Control Center  will  notify the
    Occupant  Emergency  Coordinator, who  in turn will contact the  Area  Coordinator  if  any
    evacuation  is to take place.  The Area Coordinator may  decide to evacuate the facility or vessel if,
    in their judgment, there is not sufficient time to contact the Designated Official.

6.   The Fire Protection Monitors will use fire extinguishers to control small fires.  In  case of a major
    fire, the Zone Monitor will warn persons in the immediate vicinily to evacuate the  affected  area.

7.   Immediately upon  hearing the alarm, personnel will not activate the  ventilating system, nor will it
    be automatically activated.

8.  Occupants will take the following action when the fire alarm  is sounded:

    a.   Proceed  as specified by the ship's Station Bill.

    b.   Obey the instructions of the Area Coordinator and the monitors.

    c.   Proceed  into the stairway or exit in an orderly manner as directed  by the Stairway Monitor.
    d.   Walk  to  the 0-2 deck, or  any alternate area specified  by the  Area Coordinator and  stay in
        formation.

    e.   If the  facility is completely evacuated, wait for instructions from Organization officials.

    f.   If time and safety permit, place classified documents in a safe or secure location; put exposed
        records  in cabinets or desk  drawers or  spread covers over the  records to protect  them;  and
        disconnect electrical equipment.  The last person to leave the room closes, but does not lock.
        the door.

    g.   Evacuation will utilize the routes shown on the evacuation floor  plan which  is posted in the
        Captain's office, the galley, and in the passageways of all labs.  The nature of the emergency
        may require an  alternate route.

    h.   Upon  evacuation, each Area Coordinator will report to  the  Occupant Emergency Coordinator
        located  at the Emergency Control Center. Information  given  by the Area Coordinator will
        help keep organization officials aware of the progress of the evacuation.

    i.   If a partial or  complete evacuation has been ordered, personnel will  exit according to the
        evacuation  plan.   In  all cases, personnel will obey the Area  Coordinator's  and Monitor's
        instructions.

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B - Bomb Threats and Civil Disorder
1.  Guides.  The following guides are established to assist in preparing for and minimizing the effects
    of emergencies.

    a.    Protection of  Priority Locations.   Locations  requiring priority  protection   (switchboards,
         teletype centers,  heating  plants,  etc.) should  be  identified in   advance and  arrangements
         should made for  protection  of these locations.   Consideration should  be  given to the use of
         existing heavy monumental-type metal doors, gates, etc., when available,  as physical barriers
         to deter unauthorized entry of selected priority locations.

         (1)   Planning and Inspecting.  Survey the premises of specific occupants, whose locations
              have or may reasonably be expected  to be the site of civil disorders or demonstrations,
              to determine if some   entrances to the area should be closed, and if door latches are
              adequate and tamper resistant, if the  site lends itself to installation of practical  types of
              intrusion and fire alarm systems,  or  to the installation of automatic fire  extinguishing
              systems to  minimize fire losses and deter vandalism.

         (2)   Suspicious Actions. All personnel,  particularly guards and  vessel  personnel,  shall be
              alerted  to   observe  and report immediately  any threats  and  all  suspicious-looking
              persons, packages or equipment.

         (3)   Building Service  Spaces.   All  doors to service areas,  such  as  mechanical  rooms,
              transformer vaults, slop-sink rooms,  wire closets, etc., should be  locked at all times
              when not occupied.  Access will be allowed only to authorized personnel.

         (4)   Stairwells and Restrooms.  Particular attention should be given  to closets incapable of
              being  locked,  restrooms,  stairwells and  other areas where  explosives  might  be
              concealed.

         (5)   After-Hour  Entry.   Identifying  and  recording the presence  of all persons  entering or
              leaving the  facility  or vessel after normal  hours is essential.

2. In case of a bomb threat, perform  the following actions:

    a.    Upon  learning  of a bomb threat:

         (I)   Attempt to  learn  the exact location where the bomb has been or  is going to  be  planted.
              Attempt  to  learn  as   much  information  as possible about  the   caller,  e.g.  vocal
              idiosyncrasies,   race,  sex,  approximate   age,  group affiliation,  etc.     Listen  for
              background noises or  for  other clues which might  indicate the caller's identity  and
              location. Information  received should be  carefully recorded and  immediately relayed to
              the Designated Official.

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     (2)   Persons receiving a  letter or written message concerning a bomb threat will deliver it
          immediately to the Designated Official.

b.    The Control Official or Federal Protective Service will notify the Designated Official and the
     Bomb Search and Reconnaissance Officer that a bomb threat has been received and give the
     location, if known.  The Federal Protective Service will initiate the telephone cascade system
     to alert all team members to  begin a visual  search of the reported location.  A  suspicious
     item  is never  to  be moved or  disturbed.  The telephone cascade  system initiated  by the
     Federal Protective Service is as follows:

     (1)   Federal Protective Services notifies the Occupant Emergency Coordinator.

     (2)   Occupant Emergency Coordinator alerts the Area Coordinator.

     (3)   Area Coordinator notifies the respective Zone Monitors.

     (4)   Zone Monitors search their assigned areas, including stairwells,  restrooms, and utility
          rooms off the corridors.

     (5)   Available guards, maintenance  men and personnel  report to the  Federal  Protective
          Service for  assignment.  Personnel located at their assigned area will work  under the
          direction of the Area Coordinator.   Whenever possible, searchers will work in pairs and
          move with extreme  caution, both during the search and after the bomb is located.

     (6)   Upon completion of a thorough inspection of their  areas, searchers will report to the
          Zone Monitor who contacts the Area Coordinator to report the results of the search.

     (7)   The  Area Coordinator contacts the Occupant Emergency Coordinator who notifies the
          Control Center of the results of the search.

c.    In the event a suspicious-looking object is found, the finder performs the following  actions:

     (1)   Does not  remove or disturb the suspect object.

     (2)   Immediately evacuates all persons  from  the affected area.

     (3)   Notifies the Zone Monitor and calls Federal Protective  Sen-ice Control  to report that a
          suspicious item has been located.

     (4)   The   Zone Monitor notifies the  Area  Coordinator  that a suspicious  item  has  been
          located.

     (5)   The  Area Coordinator notifies the Occupant Emergency  Coordinator that a  suspicious
          item has been located.

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        (6)   The  Occupant Emergency Coordinator notifies the Control  Center and the Designated
              Official that a suspicious item has been found and informs them of its location.

        (7)   The  Control Center  immediately notifies the  Bomb  Search  and     Reconnaissance
              Officer  that a  suspect  item has been  found  and  he/she will  verify  the item  as
              threatening or non-threatening.

    d.   The Designated Official may  order an immediate evacuation of the  area  near  the affected
        location if the suspicious item is identified as a bomb.

    e.   If searchers locate a bomb, they will notify the  Bomb Search and Reconnaissance Officer.

    f.   After disposal  of the  bomb, the Designated Official will give the signal for  occupants  to
        return to the area.

    g.   The Bomb Search and Reconnaissance Officer will  then complete the  report as shown on
        page 24.
C - Instruction Sheet For Initial Bomb Threat Report

1.   Date of threat.

2.   Time threat was received.  Indicate if a.m. or p.m.

3.   Print the  exact words, as close as possible, used by the caller.

4.   Did caller give a location where the device is supposedly planted?

5.   Did caller state when the device would detonate? If so, list time.

6.   Did caller state what type of device it was? If so, please describe.

7.   Did caller describe the device? If so, please  list description.

8.   Did caller state why the bomb was placed? If so, please list.

9.   Please describe anything that could be heard  in the background such as street noises, office noises.
    music, children  laughing, etc.

10.  Check whether  caller seemed to be  male or female and the age group that comes closest to  the
    sound of the voice.

11.  Check any mannerisms, accents, speech patterns, etc., that  could  be detected in the voice.

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12. Did you recognize the voice of the caller?  Please indicate.

13. Name of person who completed the form.

14. Immediately report the call, with the above  information, to the supervisor  or  the  designated
    organization official.

D - Bomb Threat Data Report      (Keep near Telephone)

Date Received	  Time Received	a.m./p.m.

Time Completed	a.m./p.m.

Please  try to record below the exact words  spoken  by the  informant.   Ask that the message be
repeated - keep informant talking.
Request Specific Information:

In what area is the bomb placed?
Where is the bomb placed?  	
When will the bomb explode? 	
What kind of bomb is it?    	
What does the bomb look like?
Why was the bomb placed? 	
Additional Information to be Recorded after Receiving the Threat:

Background Noises (describe):	
Voice Description:

Male           Female      	   Young  	Middle Age	Old Tone
Sober           Intoxicated  	   Nervous 	Firm

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             Accent/Impediment
             Was Voice Familiar:   Yes	  No	   If so, who

             Recipient's Name   	

             Office Address        	

             Telephone Number	
IMMEDIATELY Notify your Official in charge who in turn will:

Call Director of Facilities

Daytime Extension:

Nighttime Extension:

E - Instruction Sheet for Follow-up Report

PLACE THE TIME AND DATE  OF THE INITIAL REPORT IN THE UPPER RIGHT CORNER.
If an initial report has not been completed and a  suspicious package or bomb is found, or if a bomb
detonates, include the  agency name,  building or  vessel  name,  location and  address in  miscellaneous
(9).

1.  Include all agencies notified such as the Army Explosives Ordnance Disposal Unit, police,  fire
   department, etc.

2.  Self-explanatory.

3.  Self-explanatory.

4.  Self-explanatory. If exact number of persons is not known,  estimate number.

5.  Self-explanatory.

6.  Describe items found, who was  notified, and action taken to  remove and identify these items.

7.  Same as 6.

8.  Self-explanatory. Describe physical damage in as much detail as possible.

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9.  See first paragraph above.  Include any information not covered elsewhere on the form.

10. Other actions taken to augment security in the building.

11. Name of person completing the report.

1.  Agencies notified of initial report:	
2.  Public Area Search Conducted:     	   YES	NO

3.  Complete search of all rooms/offices conducted:	YES   	  NO

4.  Evacuation:   Partial  	  YES   	  NO  Complete  	  YES   	  NO

No. of Persons: 	   Time  Evacuation Began:	

Time Employees Returned:  	

5.  Increased Security:

Was Package Inspection/Identification Instituted:	YES	NO

Were Entrances and Exits Placed Under Control:  	YES	NO

Was Personal ID Required to Enter the  Building:	YES	NO

6.  Were Suspicious Items Found:  	YES	NO

Description of Items:	

Action Taken:                                        	
Final ID and Disposition of Items:
7. Bomb Device Found:  	YES   	  NO

Description of Items:	

Action Taken:                  	   	
Final ID and Disposition of Items:

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8. Bomb Device Detonated:  	YES    	 NO      TIME:

Date:  	    Location:	

Area:	

Number of Persons Killed: 	

9. Miscellaneous:                    	    	
10.  Other Actions Taken:
11. Recorder:
Name    Phone                     Room No.
Date/Time Position Title
Agency Address

F - Demonstrations

1.   Criminal Jurisdiction:  The handling of demonstrations,  "sit-ins",  etc.,  has  been the subject of
    discussion among  officials of  the  Department of Justice and it  has been  determined  that the
    Designated Official, assisted by the Director of Facilities  will  handle such  matters  in  close
    cooperation with the office of  the United States  Attorney in the Chicago Area.   There  must be
    mutual  understanding  and agreement regarding  the action  to be taken  in such  matters and
    assurance that such action is undertaken  with  legal authority and will not violate  civil  rights.  The
    Designated Official, assisted by the Director of Facilities,  is the point of liaison  for all tenants in
    the planning and execution of action for  handling demonstrations.

2.   If possible, the following pertinent  facts should  be learned  prior  to  demonstrations so  that an
    appropriate plan may be prepared:

    a.    Name of sponsoring organization.

    b.    Time of demonstration.

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    c.    Proposed schedule of events.

    d.    Exact location.

    e.    Activities planned.

    f.    Purpose  of demonstrations.

    g.    Projected attendance.

    h.    Character and temperament  of those expected to participate.

3.   The Designated Official  and the  Director of Facilities should meet with occupants,  local police,
    United States Attorney, United States Marshal and others as deemed necessary.

    a.    The cascade plan of communication will  be  reviewed to  ensure continuity of information
         and necessary action between  the occupants.

    b.    When  a demonstration is expected  to take place on GSA-controlled property,  guards and
         other personnel shall be instructed in accordance with the plan of action agreed  upon with
         the U.S. Attorney.   Instructions will vary.  They may include permitting the demonstration
         to take place provided that entrances are kept clear and Government property and operations
         are  not  adversely  affected;  participating  in  arrangements  of  a meeting  between  a
         representative  group of demonstrators  with appropriate  Government officials;  prohibiting
         entry into the facility by persons who parade bearing signs or who by other actions identify
         themselves  as participants;  calling on  enforcement resources  for  assistance,  etc.   It  is
         desirable to  use  procedures  which appear most likely  to  gain  the  cooperation  of the
         demonstrators.

    c.    Agreement  should be  reached  regarding  the  circumstances  under  which  arrests  may be
         necessary and the  course  of action to  be followed in effecting  such arrests.   (In many
         instances, arrests are openly sought by demonstrators.  Arrests should only be made when
         necessary.)

5.   Plans will be made as to what action  is to be taken if persons should gain entrance to the facility
    and then begin a  demonstration, either individually or as a group.  Normally,  demonstrators shall
    be asked to leave.  If they refuse, become disorderly, or offer passive  resistance, i.e., lie or sit on
    the floor, it  may  become  necessary  to  place  them under  arrest.   An  alternative to arrest  is
    removing demonstrators from  the building  and restricting  reentry.   Again,  arrests  should be
    avoided  if possible.  Necessary  arrests should be carried  out  under the guidance of the U.S.
    Attorney.

G  - Explosions

In the event  of an explosion, the following actions will be performed:

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1.   Occupants  will take cover under tables, desks or other objects which will provide a measure of
    protection against flying glass or debris.

2.   Occupants  will contact the Federal Protective Service, activate the  fire alarm system,  and then
    notify self-protection personnel.

3.   Occupants will contact the Area Coordinator to report the location of the explosion.

4.   The Area Coordinator will contact the Federal Protective  Service describing the situation as fully
    as possible.

5.   The Federal Protective Service will notify the Designated Official and  the Occupant Emergency
    Coordinator.

6.   The Federal Protective Service will use the public address system to notify occupants and describe
    the situation as fully as possible.

7.   After  the  effects of the  explosion  have  subsided,  the  Designated  Official will decide  if  an
    evacuation is necessary.

8.   If an evacuation is necessary, occupants will  evacuate  under the  direction of  the  Occupant
    Emergency Coordinator and staff.

9.   If the facility is  evacuated, occupants will wait outside the  facility for further instructions from
    Organization officials.
H - Chemical Accidents


A chemical accident of disaster magnitude would include accidents involving large quantities of toxic
gases.  Should such an accident endanger occupants, perform the following actions:

1.  Occupants will notify the Area Coordinator of danger.

2.  Area  Coordinator will  telephone the Designated Official  and describe the situation as fully as
    possible.

3.  Designated Official will decide if evacuation is  necessary.

4.  If an evacuation is necessary, occupants will evacuate under direction of the Area Coordinator.

5.  Designated  Official  will telephone the Fire Department and describe the situation as fully as
    possible.

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6.   First-Aid Monitors will administer first aid if necessary.

7.   Occupants may avoid toxic fumes by moving cross wind; never move up wind or down wind.

I - Severe Windstorms


The U.  S. Weather Bureau can forecast severe windstorms with a high degree of accuracy.  However,
if high winds develop during working hours with little or no warning, perform the following actions:

1.   Take shelter away from glass and movable objects.

2.   On the RV Lake Guardian, take direction from the Captain or Safety Officer.

3.   The Utilities Control Officer will  notify the  Director of Facilities and the Designated Official of
    any damage.

4.   The Director of Facilities will maintain contact with the National Weather Service for the latest
    weather information.


J - Earthquakes

Operations on  board the R/V Lake Guardian  in the Great Lakes  Areas are not normally subject to
earthquake, however, if one occurred, there would be little or no advance warning.

1.   Upon feeling a serious quake, occupants will perform the following actions:

    a.     Obtain shelter under tables, desks or other objects which will offer  a measure of protection
          against flying glass or falling debris.  If possible, stay at least 15 feet  away  from  windows
          to avoid  flying glass.  Step under  a doorway or into  a corridor.   Keep away from large
          overhead light fixtures.

    b.     Do not leave shelter until advised to do so.

    c.     If evacuation  is necessary, evacuate under the  direction of the Area Coordinator.

    d.     Exit  from the facility with extreme caution.  Watch  for falling  or  unstable debris,  live
          electric wires,  escaping gas, etc.

    e.     Proceed to an area  of safety.

    f.     Take additional precautions as deemed necessary.

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2.  The Designated Official will notify the Fire Department of any fires.

3.  Some of the following conditions may exist in conjunction with or following a serious earthquake:

    a.    Presence of dead and/or injured persons.  Some may be trapped  in the  facility or under
         debris.

    b.    Disorder and unrest, including panic and looting.

    c.    Fire.

    d.    Shortage or contamination of drinking water.

    e.    Shortage of water for fire fighting.

    f.    Shortage of food supplies.

    g.    Extensive damage to the structure rendering it potentially unsafe for occupation.

    h.    Disruption of gas and electric power systems.

    i.    Shortage of adequate living accommodations or shelters.

    j.    Blocked and hazardous streets, highways, roads, bridges, etc.

    k.    Major or complete disruption of public and private transportation facilities.

    I.    Danger of widespread epidemic due to water contamination and breakdown of public health
         controls.

    m.   Shortages of medical supplies and hospital facilities.

    n.    Disruption of regular governmental operations.

    o.    A large-scale evacuation from the metropolitan area.

4.  Immediately after an earthquake, Organization personnel will perform the following actions:

    a.    Administer first aid, and assist in any way with  rescue operations.  Move  seriously  injured
         to emergency treatment center.

    b.    Disconnect office utilities:  gas,  electricity and  water.

    c.    Inspect  all utility lines for damage.

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    d.    Extinguish fires.  Do not light fires after an earthquake.

    e.    Reserve telephones for emergency use only.

    f.    Be aware of hazards such  as broken glass, spilled fluids, damaged equipment,  crumbling
         ceilings, etc. Take corrective action if feasible.

    g.    If required,  request assistance from outside sources, such as civil defense, disaster corps, etc.

    h.    Monitor radio for community disaster information.

    i.    Attempt to carry  out routine procedures and return building to normal operation if possible.

K - Enemy Attack

Civil  defense  signals,  warning of an  enemy attack, are sounded by sirens operated by various cities
and counties.

1. ALERT  SIGNAL.   This  is a steady  blast or tone lasting three to five  minutes signifying that
essential emergency information will be broadcast.

    a.    If evacuation is  necessary,  occupants  will  evacuate  according  to  directions of  the  Area
         Coordinator.

    b.    Occupants will take personal possessions,  such  as  coats, purses, food, etc., when leaving
         their work stations.

2. ATTACK WARNING SIGNAL.  This is a wavering tone or a series of short blasts lasting three to
five minutes; signifying that an attack against the country has been detected.

    a.    Occupants will perform the following actions:

          1)  Take cover under desks, tables,  or any large objects which will  afford  a  measure of
             protection against flying glass or  debris.

         2)  Avoid windows.

         3)   Leave cover  only when directed by the Area  Coordinator.

         4)   Proceed to the  shelter  route  indicated  on the evacuation floor plan  posted     on the
              bulletin  board on each floor.

          5)  Remain  in shelter until  other action is directed by Shelter Official.

          6)  Designated Official  will contact appropriate authorities  regarding further  action to be
              taken.

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L - Panic

1.  Panic  Defined.    Panic may  occur during  an emergency.   Panic  is defined  as  "A  sudden,
unreasoning, hysterical fear, often spreading quickly."  Panic can render crowd control difficult if not
impossible,  but the ability to  control panic  may save lives  and greatly reduce injuries.    While
appealing to reason  may seem futile,  it may be  possible to  limit the  spread of panic  by assuming a
strong, positive leadership position or by reassuring key members of the group.

2.  Panic Deterrents.  The likelihood of panic, and  the physical  causes of panic, can be eliminated by
an effective program  of informing  tenant agency personnel  of  what  is expected  of  them  in an
emergency.   In  an  emergency, the Organization  should be prepared to  remove the injured and dead
from general view, and to clear away debris with  calmness.  Emergency preparations  should include
clearly delineated routes to be taken in evacuating or going to shelter.   In addition,  the designation
and placement of Organization personnel at locations where groups are likely to congregate will better
enable  them to  take  command  of the  emergency  and  provide calm,  decisive instructions  and
information.

3.  Antidotes to Panic.  In certain circumstances, it is conceivable that, despite emergency preparations,
a fearful, disorganized group may be on the verge  of panic.   Organization personnel should be
prepared to  deal with this situation according to the following principles:

    a.     Provide Assurances.  Reassure the group by giving information and instructions calmly.

    b.     Eliminate Unrest.  Dispel rumors. Identify troublemakers and  prevent them  from spreading
          discontent and fear through misinformation.

    c.     Exert Positive  Leadership.  Propose positive action in a firm and decisive  manner.   Stress
          what to do. rather than what not to do.

M - Physical Security of Facilities Alert Guidelines

1.   Guidelines have been established in order to better understand the responsibilities and action to be
    taken in situations which necessitate a security alert.

2.   Terms used in the guidelines are identified below:

    a.     Security Alert.  A Security Alert is an  action taken when conditions  dictate  the advisability
          of establishing security measures.

    b.     Degrees of Security  Alert.  There are three degrees of alert which can be  declared when the
          condition  has been identified:

          1) Moderate

          2) Medium or

          3) Maximum

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    c.    Conditions.  A situation can be described by one of the following three    conditions:

          1) Gray

         2) Yellow or

         3) Red

    d.    Application.  Generally, the  condition that is selected to describe a situation will determine
         the degree of security alert:

         Condition                         Security Alert

         Gray                                        Moderate

         Yellow            t                         Medium

         Red                                         Maximum

N - Emergency Procedures


A Minor Fire.  (A fire which CAN be extinguished with a portable fire extinguisher).

    1.    Do not sound the alarm.

    2.    If you know how to use the fire extinguisher, do so and then report the fire.(See Step 3)

    3.    Report the fire and its location immediately to your supervisor, any self- protection official.

A Major Fire.  (A fire which CANNOT be extinguished with a portable fire extinguisher).

    1.    Pull down the handle on the nearest fire  alarm box.

    2.    Notify  your supervisor  immediately.

    3.    If  an  evacuation  is  ordered  by  your  Area   Coordinator,  follow  the  directions  of
          self-protection personnel.

Evacuation

    1.     If there are visitors in your  area, notify your supervisor and,  if necessary,  assist in their
         evacuation.

    2.     Use the nearest stairwell.   Do not use the elevators.

    3.     If  an  evacuation  is  ordered  by  your   Area  Coordinator,  follow  the  directions  of
          self-protection personnel.

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Suspicious Objects/Persons

    1.    Report suspicious persons and/or objects to your supervisor immediately.

    2.    Never touch a suspicious object and/or package!!!

Bomb Threats

    1. Telephone Bomb Threat

         a.   When a bomb threat is called in, keep caller on the  line as long as  possible.  Attempt
             to learn where the bomb has been placed and the time it will detonate.

Prevention of Theft in the Facility

1.   The  prevention of theft  of both Government  and personal property  is  everyone's  concern.
    Personnel should be aware of the types of theft that may occur and take precautionary measures to
    minimize losses.

2.   Theft of calculators,  electric  typewriters,  and  adding machines is  sometimes  attempted  by
    concealing these  items in  cardboard containers until they can  be  removed from the facility.  No
    carton or container should be ignored regardless of how routine or innocuous its appearance may
    seem.

3.   Extreme caution  should be taken with personal items such as  purses, pocketbooks, loose change,
    etc.   Most of these thefts  occur as a  result of employees carelessly leaving such items unattended
    on desk  tops or in unlocked drawers  or cabinets.  Reductions in these occurrences can be made  if
    personnel exercise more  care  in managing  their personal  property.   All  valuables should  be
    secured  in locked containers,  such as file  cabinets, during working hours.  If valuables have been
    kept  in an office desk  for years without incident, it is no guarantee that they will not be missing
    tomorrow.  Personal items that have  monetary or sentimental value are NOT secure when  left in
    an office at night regardless of the precautions taken.

4.   THE GOVERNMENT DOES NOT REIMBURSE INDIVIDUALS FOR THE LOSS (THEFT) OF
    PERSONAL ITEMS.

5.   DO  NOT LEAVE ANY  PERSONAL BELONGINGS. THAT YOU  ARE NOT WILLING TO
    LOSE. UNATTENDED IN THE OFFICE.

6.   Suspicious or unusual situations or the loss of property should be reported  immediately.

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Fire Prevention

The  following practices for fire prevention have been established throughout the R/V Lake Guardian
in accordance with 29 CFR 1910.38:

A.  Fire Hazards And Control Procedures

    The following is  a  list of major fire  hazards found on  board the R/V Lake  Guardian and  their
    handling, storage, and control procedures:

         Combustible materials such as cardboard,  paper,  oils, etc. are used on board the R/V Lake
         Guardian on a regular basis.  Combustible materials should not be  allowed to  accumulate
         near sources of heat or flame.  They should be promptly disposed when no longer needed.

         Flammable  materials  such as laboratory chemicals, solvents, paints,  products, etc. are  used
         on board  the  R/V  Lake Guardian  on  a regular  basis.   They  should always be segregated
         according to chemical  compatibility and  they should never be stored near heat or flame.
         Bulk flammable materials are stored in the flammable  materials storage locker  and should
         only be removed when needed.

         Oxygen and acetylene bottles are stored on the  "Foscle Deck" when not in use. Smoking
         and  open flames are  prohibited  in  this area.  Hoses should be rolled up when  not  in use.
         Tank valves should be closed completely and safety caps installed when not  in use.

         Acetone is  frequently used in the laboratory areas on  board  the R/V Lake Guardian.  No
         more than 4.0 liters of acetone are allowed at one time  in any  laboratory area. Acetone and
         other flammable laboratory  chemicals  should  always be handled and transported inside of
         secondary containment devices.

         Laboratory  heat sources (e.g.  electrical  appliances)  are  used on  board the  R/V Lake
         Guardian and must be inspected on a regular basis  to ensure they are  operating properly.

         Electrical cords used on board  the R/V  Lake Guardian, must  be  inspected on a  regular
         basis.   Damaged cords should be replaced or removed  from  service to prevent  fire and/or
         injury.

         Gasoline  fuel  sources exist on the 0-2 deck and around  equipment on the fantail.  Care  must
         be exercised in those areas to minimize heat sources.

          Heat and fuel sources  exist in the Galley area (e.g. stove burners,  oven cook tops, coffee
          pot, paper,  cooking  oil,  etc.).   Care should be taken  to keep combustible and flammable
          materials away from  heat sources at all times.

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         Smoking areas have been established on board the R/V Lake Guardian  in outside areas at
         least 10 feet away from flammable fuel sources.  The areas have been designated in part to
         minimize the chance of fire.

         Portable  fire  extinguishers,  fixed  extinguishing systems,  smoke  detectors,  emergency
         lighting,  and  notification  systems have  been  installed  in areas throughout the R/V Lake
         Guardian.   These  systems are  regularly  inspected  in  accordance  with  a  facility  fire
         inspection program.

B.  Responsible Personnel

    The following personnel are responsible for maintaining fire control  equipment and fuel source
    hazards located on board the R/V Lake Guardian:

         Facility Fixed  Extinguishing Systems;  Ship Operating Contractor

         Facility Alarm System;  Ship Operating Contractor

         Facility Portable Fire Extinguishers;  Ship Operating Contractor and Ship Contract Chemical
         Hygiene Officer

         Emergency  Lighting Systems;  Ship Operating  Contractor  and  Ship Contract  Chemical
         Hygiene Officer

         Smoke Detectors;  Ship Operating Contractor and  Ship Contract Chemical Hygiene Officer

         Fuel Source Hazards (e.g.  flammable or combustible materials);  All facility personnel

C.  Housekeeping And  Fire Prevention Procedures

    It is the intent  of  this section to make sure that hazardous  accumulations of flammable or
    combustible materials are controlled so that a fast developing fire, rapid spread of toxic smoke, or
    an explosion will not occur.

    The  following  housekeeping  procedures  must  be  followed  to  prevent  the  accumulation  of
    flammable and combustible materials and waste residues on board the R/V Lake Guardian:

    1.    Maintain good housekeeping in  all  areas;  this  is one of the most effective means of
         preventing  fire.

    2.    Bring to the attention of your supervisor any apparent existing fire safety hazard.

    3.    Obey "No Smoking" signs.

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    4.    Do not toss matches, cigars,  cigarettes, or pipe ashes into  waste baskets or any type of
         receptacle containing  combustible material.

    5.    Oily  rags  or  similar flammable materials  must be  placed  in  approved  metal containers
         provided for that purpose.

    6.    Coffee  pots, microwave  ovens,  hot  plates, or similar  electrical equipment with heating
         elements may be used  if such  appliances are approved by  Underwriters'  Laboratory and
         installed in accordance with  local fire  codes  and the Director of Facilities' approval.

    7.    Deposit all trash  in receptacles provided for  that purpose or arrange for its storage in regular
         trash  rooms.

    8.    Exits, passageways,  and  lobbies  must be  kept  clear  of stored material to permit  free
         movement of personnel  and  fire-fighting equipment.

D.  Maintenance Procedures

    The  following maintenance  procedures have  been  established for  the  R/V Lake Guardian
    heat-producing equipment to prevent accidental ignition of combustible materials:

         All heat-producing equipment used throughout the R/V Lake Guardian must be  used and
         maintained in accordance with manufacturer  specifications.

         Prior to  using  any  heat-producing  equipment,  supervisors  and/or operators  should be
         thoroughly familiar with all  equipment operation and maintenance procedures.

         Defective heat-producing  equipment  should be removed from  service until  repairs can be
         made.

         All fire extinguishing  systems, emergency  lighting,  smoke detectors,  and  alarm systems
         installed on or near heat-producing equipment must be regularly  inspected by ship personnel
         in accordance with established inspection procedures.

E.  Employee Training And Awareness Programs

    R/V  Lake Guardian personnel are apprised of the fire hazards of the  materials and processes to
    which they are exposed during regularly scheduled training sessions. The training sessions offered
    to R/V Lake Guardian personnel consist of  the following:

         Regular Fire /Evacuation Drills (noted in the ship's  log)

         Initial  Orientation Program offered   by  the Contract  Chemical Hygiene  Officer  which
         consists of the contents  and  location of the Occupant Emergency  Plan/Fire Prevention  Plan

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         Initial Orientation Program offered by the Captain and/or First Mate which consists, in part,
         of  general  principles of fire extinguisher  use,  fire  alarm station  locations, emergency
         notification  system  operation  (e.g.  911),  emergency  alarm  signals,   and evacuation
         procedures.
First Aid

1.  Purpose

    a.    This Standard Operating Procedure is intended to provide guidance for Federal  personnel in
         the event that emergencies arise that require emergency First Aid or Self-Help.  It is not the
         intent of this procedure to delineate the specific and various methods of giving First Aid or
         Self-Help, but rather to indicate where they are to be administered and by whom.

    b.    The procedures outlined herein pertain to  emergencies arising from disasters only.

2.  Responsibilities.  Self-Help and  First Aid training will be provided on request.

3.   First Aid Treatment Stations.   The following areas  on board the  R/V Lake  Guardian have been
    designated as First Aid treatment stations in the event of a general emergency:

         1.  The Bridge
         2.  The O-2 Level
         3.  The Captain's Office (primary)

4.  Procedures in General  Emergencies

    Procedures noted in the R/V Lake Guardian Station Bill that  is posted throughout  the vessel must
    be followed.

5.  Evacuation of Casualties from Facility

    a.    Contact the U.S. Coast Guard

    b.    Evacuation of casualties will  be made by the Police or Fire  Department Emergency  Service
         once the vessel arrives in  port.

    b.    Evacuation of casualties will be under the direct supervision of the Medical Officer.

6.   Disaster  Emergencies  or emergencies  involving  20 casualties  or more will   be  treated  in the
    following manner:

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    a.     Response  personnel,  depending  on  the type  of emergency, will  be dispatched  to the
          emergency site upon notification of a disaster type emergency.

    b.     Contact the U.S. Coast Guard.  The Coast Guard  will notify the  pppropriate authorities
          ((e.g., Police Department, EMS, etc.) and have them available once the vessel arrives in
          port.

Utilities Control

1. In case of emergency, utilities personnel will proceed  to the following assigned stations:

    a.     Electrician to sub-station master switch.

    b.     Operating  Engineer to water control point.

    c.     One mechanic to gas control point.

2.  Upon direction of the Utilities Officer, all electrical current, water service, gas and steam supplies
    will be turned off when deemed necessary for preservation of life or property.                          ^

Civil Defense Coordination

1.  Pre-Emergency.   The Designated Official and the Director of Facilities will establish and maintain
    liaison with  local Civil Defense authorities in  order to assure that this  plan  is at all  times in
    agreement with  current Civil  Defense concepts and local planning.  Revision to this plan will be
    made as  necessary  to  assure that  preparations  for  protection of life  and property are  never
    outmoded.

2.  During Emergencies. In the event of a disaster, the  Designated Official  and Director of Facilities
    will  reestablish  contact with  local Civil  Defense authorities, at the earliest possible time,  by
    telephone, radio  or messenger, as feasible  under existing conditions.  Information  and assistance
    will  be  sought   as  necessary  and,  reciprocally, the Facility  Self-Protection Organization will
    provide assistance to  Civil Defense authorities as soon as conditions permit.

Tests and Drills

1.  Policy.   The Self-Protection Organization will conduct such drills as are considered necessary to
    test and improve the various phases of this plan.

2.  Approach. The  Director of Facilities, under the guidance of the Designated Official, will schedule
    two building-wide drills each year.

    a.     Participative Drills.   In employee  participation drills,  the  employees will  evacuate the      M
          facility in  accordance with the evacuation plan  and will then return to their original area.          ^

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    b.    Non-Participative Drills.  This drill consists of a test of the alarm  system to  ensure that all
         personnel can hear the alarm signals and that all alarms are   functioning properly.

3.   Advance Notice.  The Director of Facilities will distribute written notice to all tenant agencies  in
    advance of any drills or tests, in order to assure that all employees  are positively aware that no
    actual  emergency  exists  when  warning  signals are sounded.  Agencies are  to  notify  their
    self-protection personnel of any scheduled drills.

Occupant Emergency Plan

The purpose of this plan  is to provide instructions to  be  followed in  the event of an emergency
occurring on board the R/V Lake Guardian.

The protection organization personnel in the plan will:

    •     Direct and supervise the evacuation of the vessel or building and/or movement to the shelter
         areas  in the event of a disaster.

    •     Conduct drills to  familiarize all employees with  actions to be  taken  in the  event of an
         emergency.

    •     Provide training of personnel necessary to staff the various protection teams.

         Coordinate action with local Fire Department, Police Department, Rescue Squad, etc.
O - Occupant Emergency Personnel


Occupant Emergency Coordinator:

Alternate Occupant Emergency Coordinator:

Emergency Control Center:

Area Coordinators                                    Name                Phone #

P - Protection Plan Personnel


Official                           Title                          Phone    Room

Occupant Emergency
Coordinator

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Alternate

Medical Officer

Fire Marshall
Alternate

Utilities Officer
Alternate

First Aid Team

Utilities Control
Group

Q - Personnel to be Contacted in the Event of Emergency

Name                                    Area
                             Phone #
ADDITIONAL EMERGENCY TELEPHONE NUMBERS
Local Fire Department

Emergency Ambulance Service
Bay City, Michigan

Bay City, Michigan
911 or 517-892-8601

911 or 517-894-3122

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APPENDIX N: HEALTH AND SAFETY MANUAL U.S. EPA R/V MUDPUPPY

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HEALTH AND SAFETY MANUAL
    USEPA R/V MUDPUPPY
         September 1996

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                            CONTENDS

INTRODUCTION

BACKGROUND & PROCEDURES

TRAINING, CERTIFICATION, MEDICAL MONITORING

COMPLIANCE REVIEW AND STANDARD OPERATING PROCEDURES

RESEARCH VESSEL HEALTH & SAFETY
   Health & Safety Procedures During Sediment Sampling
   Hazard Communication
   Personal Protective Equipment
   Personal Flotation Devices
   First-aid Equipment •
     Fire  Fighting Equipment
     Safety Tests and Drills
     Weather

MARINE SAFETY
     Safe  Boat Operations
     Load  Handling Equipment
     Distress Signals
     Communication

ENVIRONMENTAL COMPLIANCE
APPENDICES:

APPENDIX A:     Chapter   2,    GLNPO   Health,    Safety    and
                     Environmental Compliance Manual

APPENDIX B:     R/V  Mudpuppy  -  Health  and  Safety  Procedures
                     During Sediment Sampling

APPENDIX C:    ' R/V  Mudpuppy  -  Personal  Protective  Equipment
                     Policies & Procedures

APPENDIX D-.     SOP For Sampling Or. Board R/V Mudpuppy

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APPENDIX E:

APPENDIX F:

APPENDIX G:

APPENDIX H:

APPENDIX I:

APPENDIX J:
R/V Mudpuppy - Hazard Communication Program

R/V Mudpuppy - Chemical Inventory & MSDSs

SSI Occupational Safety And Health Manual

R/V Mudpuppy - Confined Space Entry Program

R/V Mudpuppy - Site Safety Plan

R/V Mudpuppy  -  Standard for Workers  Exposed  to
     Hot Environments

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INTRODOCTIOH

It is the policy  of  the  USEPA,  Great Lakes National  Program  Office
(GLNPO),  that  all EPA employees  working  in offices,  laboratories,
field  activities,   and  special   programs  are   entitled  to   a
comprehensive health and safety program.   The goals  of  the program
are to ensure  that each  employee  is able to work in  an  environment
free of recognized hazards  and to reduce and mitigate occupational
accidents  as  much   as  possible.    These  goals  are accomplished
through the  development  of  standard operating procedures,  training
employees in the proper techniques  for  field  investigations,  proper
use  of  and  access  to  safety eo^aipment,  and proper protocol  for
inspections.   This manual  provides standard operating  procedures
and  guidelines  relative  to  the  health  and   safety  of persons
involved in activities  conducted on board the  R/V Mudpuppy.   The
procedures  outlined  within  this  manual  must   be  used  only  as
supplemental   information    to   the  GLNPO  Health,   Safety  _ and
Environmental   Compliance    Manual   which   establishes    policies,
procedures, responsibilities, rules  and guidelines  to be adhered to
by all GLNPO vessels.
BACKGROUND & PROCEDURES

As   a   follow  through  to   the   Assessment   and  Remediation   of
Contaminated   Sediments   (ARCS)   Program,   the   GLNPO   Sediment
Assessment Program  is  designed to evaluate sites within the  Great
Lakes basin where the possibility  of  sediment contamination exists.
Most of  the  work is done in  one of  the  42 Areas of Concern (AOCs)
which are designated areas which have some known  sediment problems.
Funding  for  these  assessments  comes  mostly from GLNPO and  sample
collection  is  often  done   from   the  R/V  Mudpuppy.   The   sampling
activities  are always  different  in  scope, as  GLNPO's  goal is  to
serve the grantee's  (usually  a Great Lake State or Tribe) needs.

The  R/V  Mudpuppy participates  in  sampling  activities  where  the
expected exposures  are unknown or only  suspected  (e.g., screening
level and wide area assessments)  and where there is some knowledge
of  the   types  and  levels of  potential contaminant exposure  (e.g.,
hotspot  analyses).  However,  sediments are  dynamic and  heterogeneous
in  nature.  .  Therefore, during  sediment  sample  collection,  field
personnel fltijlf be exposed to  sediment which represents  a chemical or
biologicalffipazard.    From   a personal  exposure  perspective,   all
sediments iBfeuld be handled  as potentially hazardous  unless  there
is  scientific  evidence  to prove otherwise.

Sediment collection activities  involve taking grab  samples  and long
cores.   Grab samples are collected with a Por.ar  dredge by  lowering
it  to  the bottom with  a winch,  retrieving the Ponar,  and  emptying
the sediments into  a  large  tray  or  bowl.   The  sample is  usually
transported  to a shore-based laboratory station  for  homogenization

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and  subsampling.     However,   occasionally,   homogenization   and
subsampling  is  done on  the  forward  deck of  the Mudpuppy.  Either
way, all leftover  or slopped mud is hosed into the water  while  the
vessel is  still  in an anchored  position.   Utensils are cleaned by
hosing off and scrubbing  with a brush.  If necessary,  equipment is
decontaminated with  solvents and/or dilute acid.   All solvent  and
acid rinsates  are collected in separate,  labelled containers  and
disposed of by the grantee.

Core samples are  taken  with  a Rossfelder  P-4  vibro-corer.    The
sampling device  usually  has  a  10-15'  aluminum whi~h contains  a
butyrate core liner.  The core liner is extracted and  can be  sawed
into sections of any length.   The  core  (or core  sections)  is  (are)
capped,  taped,   labelled  and  sent  to  a shore-based laboratory
station where they are characterized and subsampled.  Deck clean-up
involves hosing and scrubbing  to remove  slop.

Sampling personnel aboard the R/V  Mudpuppy will  generally include,
at  a minimum,  one USEPA/GLNPO employee,  two  contractors, and  one
grantee.   In the  absence of  an EPA Chief Scientist,  EPA will  be
responsible  for  designating an  acting  Chief  Scientist.   Personnel
responsible  for boat operations will have  experience in small craft
vessels, as  well as  in the use of  sampling equipment.  Individuals
from  EPA  and  the  contractor participating  in  sediment   sampling
activities must meet the  training requirements described in Chapter
2 of the GLNPO Health,  Safety  and Environmental Compliance Manual.
A copy of  these requirements can  be  found  in Appendix A of this
manual.    At  a  minimum,   individuals  participating   in   sampling
activities must have 24 hour Field Safety Training or  equivalent.
Due to the nature of sampling  activities on board the R/V  Mudpuppy,
individuals  participating  in sampling activities must  be  at least
eighteen years of age.

Prior  to commencing  field operations,  the field team should gather
all site data available and assess  the anticipated hazard  level  of
sediments  to be  sampled.   When  assessing  the  sediments' hazard
potential  for  a  site,  the  depth  sampled below the  surface must
always   be  considered   because   many   Great   Lake  sites    are
characterized by  having   sediments  which  are more  contaminated  at
depth.   For  example, if historical  data available for a site comes
from a Ponar dredge, and samples were  found to be contaminated,  all
samples  below  the sampling depth  must  be treated  as  potentially
hazardous.

Due to the nature of sampling  activities on board the R/V  Mudpuppy,
stringent  health  and  safety  procedures  have  oeen  implemented.
Health  & Safety Protocols  developed  for  the  R/V Mudpuppy can  be
found  in Appendix B.

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Personal Protective Equipment  (PPE) procedures  shall  be implemented
by all  personnel working  with contaminated  sediment.    Guidelines
for the  selection and use of PPE  are  set  forth in Appendix  C,  R/V
Mudpuppy- Personal Protective Equipment Program.

Sediment assessment and tributary monitoring may entail  collecting
long  core   and  grab   samples   with  low   to  high  amounts   of
contamination.   Sample collection procedures must  be performed in
accordance  with developed  Standard  Operating  Procedures found in
Appendix D.
TRAINING, CERTIFICATION, MEDICAL MONITORING

GLNPO training courses encompass a wide range of criteria including
laboratory safety training, radiation safety training,  field health
and  safety,  first  aid and  CPR,  respiratory  protection  training,
emergency  response  training,  fire  fighting,   crane  and  derrick
operations,  occupational  noise  exposure,  hazard   communication,
bloodborne  pathogen,  etc.    It  is  the  policy  of GLNPO that  EPA
personnel   and   research    vessel   occupants,   including   ship
contractors, laboratory  contractors,  and visiting scientists,  have
appropriate  safety  training  specific  to  their  job  function  in
accordance  with  Chapter  Two  of   the  GLNPO   Health,   Safety  and
Environmental Compliance Manual (See Appendix A).

The  GLNPO Safety  Manager will  record  and certify  that annually
required  training  courses  have  been attended by  all  EPA  field
personnel.   The  GLNPO Safety Manager will  also be responsible  for
maintaining  records regarding annual  training,  medical  monitoring
and  respirator  fit  testing  certification  for appropriate  field
personnel.

The  Regional Medical  Monitoring  Selection Policy to be  followed by
all  EPA Region V  employees   is detailed  in Regional  Order  3130.
This  program is  designed to  monitor  the  health of employees  whose
work  regularly or periodically poses the possibility  of  exposure to
hazardous materials.
 COMPLIANCE REVIEW AND STANDARD OPERATING PROCEDURES

 All  personnel aboard GLNPO research vessels are required  to  comply
 with all  applicable OSHA,  USCG,  and EPA  regulations.   Health,
 safety  and environmental  compliance standards  for  GLNPO  research
 vessels  are specified in  the GLNPO  Health, Safety  and Environmental
 Compliance Manual.

 Written  health and safety Standard Operating Procedures  (SOP)  must
 be developed  for  specific  tasks  related  to  field  or  laboratory

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operations.     Within  these   SOPs,    all   safety,   health   and
environmental  compliance  issues  should be  addressed.    Standard
Operating  Procedures   for  the  R/V  Mudpuppy  can  be   found  as
appendices to  this  manual.  Additionally, Region  V and GLNPO  have
developed SOPs which may be used to supplement laboratory  or  field
SOPs   (refer   to   the   GLNPO   Health,   Safety  and  Environmental
Compliance Manual).  For convenience, portions of  the GLNPO Health,
Safety, and Environmental  Compliance Manual have been extracted and
appear in the appendices of this manual.


RESEARCH VESSEL HEALTH & SAFETY

Health & Safety Procedures During Sediment Sampling

When  handling  sediments of unknown  contamination,  all  sediments
must be treated as potentially hazardous unless scientific  evidence
proves otherwise.   To  reduce  the potential  of exposure, health and
safety procedures during the handling  of contaminated sediments  is
of utmost importance.   This manual contains policies and procedures
which have been developed  and must be  implemented during  sediment
sampling.

GLNPO  has established  a  protocol  which implements   the  minimum
acceptable  health  and  safety  standards  for  the  protection  of
persons aboard EPA vessels.  All  personnel  aboard  GLNPO   research
vessels  are  required   to  comply  with  the  health,  safety  and
environmental  regulations   in  accordance  with  the GLNPO  Health,
Safety and Environmental Compliance Manual.   Protocol requirements
developed specifically for the R/V Mudpuppy,  in accordance with the
GLNPO  Health,   Safety   and Environmental  Compliance  Manual,  are
contained in the appendices of this manual.

Hazard Communication

In  accordance  with OSHA's Hazard Communication  Standard  (29  CFR
1910.1200)   an  employer   is   required  to   develop   a   Hazard
Communication  Program.      A  successful  program  requires  the
cooperation of EPA  and  contracting personnel.   It is  the  duty of
GLNPO to assure that Hazard Communication  Programs are  implemented
for all research vessels.  The Hazard Communication Program for the
R/V Mudpuppy can be found in Appendix E of this manual.

Hazardous  materials  used  aboard  the  R/V  Mudpuppy  include  such
chemicals as organic  solvents, corrosives,  flammable liquids,  and
toxic  or  reactive chemicals.   A  Chemical  Inventory and  Material
Safety  Data  Sheets   (MSDS)  for chemicals  used  aboard  the  R/V
Mudpuppy can be found  in Appendix F of  this manual.   MSDSs must be
available  during   all  sampling  operations.     MSDSs   provide

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information  necessary for  immediate  response  in  the  event  of  a
spill or personal exposure.

Personal Protective Equipment

It is the policy of the EPA to protect the health and  safety of  all
personnel, especially  those persons  engaged in work of  a  hazardous
or  toxic nature,  by  providing  and  requiring  the use  of proper
Personal  Protective   Equipment   (PPE).     A  Personal  Protective
Equipment Program has  been developed for the  R/V Mudpuppy  and  can
be found in Appendix C.

Sediment  assessment  and sampling  may entail  collecting long core
and grab samples with low to high amounts of contamination.  Sample
collection   procedures   must   be  performed   in  accordance  with
developed Standard Operating  Procedures  found in Appendix C & D.
The  SOP's   contain   listings  of.  PPE  required  during   sampling
procedures.

To minimize  the  risk  of  exposure proper decontamination procedures
must be  followed during  the donning  and doffing  of PPE.   These
procedures are outlined in Appendix C of this manual.
Donning and doffing procedures specific to those procedures  used  on
the Mudpuppy can be found in Appendix C.

Additionally, Chapter  6 and Appendix  I of the GLNPO Health, Safety
and   Environmental   Compliance   Manual   establishes   policies,
responsibilities,  and  practices  for the  procurement,   issuance,
control and use of PPE by GLNPO employees.

Personal Flotation Devices

Each EPA vessel must  be  equipped with  Personal  Flotation  Devices
(PFDs)  for at least 110 percent of the vessel's maximum complement.
  PFDs  must  be  distributed  throughout  the  vessel  and  readily
accessible.   Each PFD must be clearly stenciled with the vessel's
name.   PFDs  (work vests/type III) must  be  worn  at all  times when
working  on  the  open deck  (see  Appendix B for  additional safety
equipment requirements).

First-aid Equipment

Medical  first-aid kits shall be provided aboard  all EPA vessels.
The  first-aid   kit   shall  be  stocked   in  accordance  with  the
requirements  found  in Appendix B.   The  first-aid kit must be well
organized,   inspected  and  up-dated  at   least  monthly.     Expired
materials must be replaced and disposed  of  immediately.   A  list  of
contents  and any necessary user  instructions must be posted inside
the  first-aid kit.

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In  the case  of personal  illness  or  accident deemed sufficiently
serious by  the  Shipmaster  to  warrant treatment beyond the first aid
facilities  on board,  the Shipmaster must be familiar with and shall
follow the  Medical Emergency  Procedures outlined in  Appendix  B of
this Manual.

Fire Fighting Equipment

Fire prevention programs  must be established  for  all  GLNPO vessels
to  reduce  the  possibility  of fire.    If  fire  does  occur,  well-
developed   fire  fighting  procedures  will  be  implemented.    The
Shipmaster  is  in  charge  of  fire prevention  and  protection.   The
Shipmaster    will    establish   inspection   schedules,    provide
recordkeeping,  and  ensure  that  all  fire  fighting  equipment  is
properly inspected and in working order  in  accordance  with Appendix
B of this manual.

Safety Tests and Drills

It  is  the  responsibility  of  the Shipmaster to  conduct and  record
safety tests of equipment as well as procedural drills. Testing and
drill  requirements can be found in Appendix B  of this  manual.

Weather

Safe boat   operations  as  well  as personal  safety can  be greatly
enhanced by establishing  operational  limitations  based on weather
conditions.   Staying abreast  of  current and developing conditions
is  the  responsibility  of  the  Shipmaster.    Maximum   operating
conditions  are  discretionary  and  final determinations  should be
made  with  consultation  of  the  USEPA   Chief Scientist  and   the
Shipmaster,  the Shipmaster having final authority.

Additionally, personal  exposure  to  weather conditions can  pose a
threat to  life  and safety.   Working in  hot and  cold environments
can  lead  to injury,  illness  and death.   The Shipmaster  and  the
USEPA  Chief Scientist must be aware  of  the signs and symptoms of
hypothermia and  heat stroke.   If at any time  conditions  are such
that they lead  to impaired judgement  and performance, unsafe wor'-:
practices,   or render  a  threat to human health and life,  it is  the
Shipmaster1^  responsibility  to   deem  conditions  unsafe   for  wor--.
activities.
MARINE SAFETY

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Safe Boat Operations

In  addition to use  of personal  flotation  devices,  man  overboard
procedures,  fire  safety,  weather  awareness,  distress   signals,
fueling procedures,  and rules  of the road,  small boat operations
include embarking,  anchoring,  and docking  activities.   Small  Boat
Standard  Operating  Procedures   have  been  developed  by  Seaward
Services  Incorporated  (SSI)  and  can be found  within the  document
titled "SSI Occupational Safety and  Health  Program Manual"  found in
Appendix G of this manual.  Additionally, GLNPO has developed small
boat handling  procedures which  can be  found in  Chapter  5 of  the
GLNPO Health, Safety and Environmental Compliance  Manual.

Load Handling Equipment

Many heavy  or  bulky items of  research  equipment  are handled  over
the  side  of a vessel  during  sampling  activities.    All  handling
equipment  involved in  sampling  activities  must   be  installed  and
operated   in   accordance   with   applicable   specifications   and
regulations.   Operating limitations should  be  clearly posted,  and
operators of winches  and cranes  must receive adequate training  in
accordance  with   regulatory   requirements.     Equipment  must   be
visually  inspected  prior to   each use,   and  formally   inspected
periodically (annually) .  If damage  or  line stress is evident,  the
equipment must be removed from service until replaced or repaired.
See Appendix B for additional requirements.

Distress Signals

GLNPO  research vessels must be  equipped with distress  signal  in
accordance  with Appendix B of  this manual.   Additionally, visual
hand signals  may  be  used to  attract attention  during a  time  of
distress.    Channel 16  on the marine radio  is the channel  reserved
for emergency  transmissions.    If  a  "MAYDAY" distress  call is heard
from a vessel,  allow  the  Coast Guard  to  respond.    If  a GLNPO
research vessel is in  a position  to render  assistance,  contact  the
Coast Guard as  soon as  there is a break  in  radio  traffic.   The  law
requires that when possible and appropriate, assistance be  rendered
to other boaters in distress.
Adequate  rq£io communications  are  essential  to  safety  on  board
research vessels.   All research vessels must have appropriate radir
equipment, even if it is not legally required.   Certain vessels are
required  to  carry  radio  communication  equipment  and  licensed
operators  in  accordance  with  47   CFR.    Federal  Communicatior.5
Commission  Rules  and  Regulations are  also applicable.    Part  ='.
governs maritime radio services .   Research vessels must be equippe :
with sufficient numbers  of long-range and  short  range  radios wi-.r.

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adequate  channels  and  power  output  to   insure   transmission  of
operational  or  emergency messages,  and  the  receipt  of  weather
information.   A  vessel  should not  deploy without  at  least  two
working means of communication.
ENVIRONMENTAL COMPLIANCE

Executive Order 12088, signed by President Carter in  1978,  requires
Federal Agencies to comply with all requirements of federal,  state,
and  local  environmental  regulations.   As  such,  EP\ regions must
comply with these  regulations  concerning  air,  water,  hazardous  and
nonhazardous  waste,  toxic  substance  control,  emergency planning,
and  community  right-to-know.     Each   division  is  required   to
establish  practices   and  procedures  which   comply  with   these
regulations as  they relate  to  individual  operation.   Specifically,
GLNPO  personnel   must   understand and  comply  with  regulations
detailed in the Toxic Substance Control Act  (TSCA) and the  Resource
Conservation  and  Recovery  Act  (RCRA)   (Chapter  9   of   the   GLNPO
Health, Safety and Environmental Compliance Manual).

Additionally,    all EPA  employees,  including USEPA contractors,  are
required to act in an environmentally responsible manner  to protect
themselves, the public,  and the environment.

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USEPA R/V MUDPUPPY
    APPENDIX A

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                                               SECTION: CHAPTER 2,£<-AifO /fa*,
                                               VERSION: FINAL/AUGUST 1993
                                               PAGE: 2 -1

 Chapter 2 - TRAINING. CERTIFICATION. AND MEDICAL MONITOR INC,


 REQUIRED TRAINING POLICY - OVERVIEW
 It is the policy of the Great Lakes National Program Office that all EPA personnel
 and research vessel occupants, including ship contractors, laboratory contractors,
 and visiting scientists, have appropriate  safety training  specific to  their job
 functions in accordance with EPA requirements.  All required  courses will be
 provided by the EPA Safety Manager, with the exception of Superfund training.
 (Superfund training will be provided by the Office of Superfund.) Training courses
 will be offered on an annual basis.

 GLNPO  training courses encompass a wide range of criteria including laboratory
 safety training, radiation safety training, field health and safety, first aid and CPR,
 respiratory protection training, emergency response, fire fighting, crane operation,
 industrial truck operation, occupational noise exposure, hazard communication,
 confined space  entry, hot  work and lockout-tagout procedures,  as well as
 bloodborne pathogen training. In addition to training courses, GLNPO adheres to
 the regional medical monitoring selection policy followed by all EPA Region V
 employees.
2.1. Required Training Policy

All EPA and Contractor personnel who work in EPA owned or leased laboratory
space (including laboratories on research vessels, and all field locations) must meet
the safety training requirements specified in EPA Order 1440 and Regional Safety
Manuals.   These Regional Safety Manuals must be readily accessible at  all
locations.  Supervisors must assure that  their employees are aware of these
manuals  and that the  requirements within the manuals will  be  implemented.
Chapter  Eight  of EPA  Order 1440 specifically  addresses working with toxic
substances in laboratories. Regional and laboratory safety regulations are designed
to comply with the OSHA regulations 29 CFR Part 1910 "Occupational Exposures to
Hazardous Chemicals in Laboratories".  All laboratories are required  to have a site-
specific safety manual. The manual should include general laboratory safety rules,
fire escape plans, emergency plans,  environmental compliance  rules, and a
chemical  hygiene  plan.   All laboratory personnel  must receive training based  on
the content of the  manual and must know its location for reference purposes.

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                                               SECnON: CHAPTER 2
                                               VERSION: FINAL/AUGUST 1993
                                               PAGE: 2-2
2.1.1
As specified in Order 1440, Chapter Eight, a 24-Hour Laboratory Safety Course must
be completed by all EPA and contractor personnel prior to working in an EPA
laboratory on a full-time or part-time basis. The 24-hour Laboratory Safety Course
is designed to cover all aspects of laboratory safety.  New employees must receive
on-the-job  training including  fire evacuation  procedures,  waste  disposal
procedures, protective equipment requirements, the location of eyewashes, fire
alarms, spill control stations, and any site-specific safety rules.  Topics such  as
potential  sources  of exposure, adverse health  effects, work practices and
engineering controls, environmental and medical  monitoring procedures, and
storage of incompatible materials should be covered, as well. Additionally, since
all laboratories generate wastes, and some of theses wastes are hazardous under
the RCRA regulations, the 24-hour training must include information regarding
environmental compliance.  This will  ensure that laboratory personnel will
understand compliance according to RCRA regulations  and how it relates to their
work practices. For further information, please refer to EPA Headquarters Order
3500.1 entitled 'Training and Development for Compliance  Inspectors/Field
Investigators".  In the event a new employee begins  work in a laboratory prior  to
scheduled training, the employee should review the materials  provided for the
Laboratory Safety Class and refresher class. The employee should then be enrolled
in the first available Laboratory Safety Class.

A training agenda  for the 24-hour course can be found as Attachment 2.1 of this
chapter.

"General Laboratory Safety Rules" appear as Attachment 2.2 of this chapter.

2.1.2 4-Hour Laboratory Safety Refresher Course

Following th^qmpletion of the 24-hour Laboratory Safety Course,  an annual
refresher coq^fcis required  thereafter.   The 4-Hour Laboratory Safety Annual
Refresher Cotflrb mandatory for all EPA and contractor personnel who work in
EPA laboratories and is designed to review safety topics for personnel who already
have a basic understanding of safety rules.  Recent advances  in health  and safety
regulations  should be included during this refresher  training.   The 4-Hour
Laboratory Safety Annual Refresher Course agenda appears as Attachment 2.3 of
this chapter.

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                                                SECTION: CHAPTER 2
                                                VERSION:  FINAL/AUGUST 1993
                                                PAGE: 2-3

 2.1.3  GLNPO Research Vessel Laboratory Training  Requirements

 In  addition  to the 24-hour  Laboratory Safety  Course and the annual 4-hour
 Refresher Course, all  laboratory personnel are required to attend a "site-specific"
 training orientation course prior to engaging in laboratory activities.  This course
 is intended to familiarize EPA personnel and contractors with the laboratories in
 which they will work. An Annual Refresher Course is required for this training
 and participants are  required to document attendance.  The general training
 agenda for the course appears  as Attachment 2,4 of this chapter.
2.1.4 Radiation Safety Training  .

The U.S. Nuclear Regulatory Commission's Material License issued to the U. S.
EPA Central Regional Laboratory (CRL) allows the limited  use of radioactive
materials  aboard GLNPO research vessel laboratories.  This license also allows
GLNPO laboratories to use Carbon  14 and Nickel for  laboratory research and
sample analysis.   While  these  materials may be used  on a  limited basis,
Radiological Health and Safety  Plans, in accordance with the  U.S. Nuclear
Regulatory Commission (NRC) Regulatory Guide S.flr and DOE publication PNL-
6577, will be  implemented to  maintain  the lowest  minimum exposure  to
personnel.  Use of these materials are subject to the guidelines specified in the
CRL Radiation Safety Manual as described in Appendix J of  this manual. GLNPO
research  vessels must also follow the guidelines presented in the Chemical
Hygiene Plan described in Appendix J of this manual.

A site-specific Radiation Safety Training Course is required for GLNPO personnel
and contractors prior to working in a  GLNPO facility where  radioactive materials
are used.   This training must provide information on work operations using
radioactive materials, personnel monitoring, protective clothing, and bioassay.
These  training requirements appear as part of  the Chemical Hygiene Plan
described  in Appendix J of this manual and radiation safety SOPs appear as an
attachment to the plan.
2.1.5 40-Hour and 24-Hour Field Health and Safety Training

Health and Safety training is required for all EPA field personnel.  The training
consists of a 40-hour Superfund Hazardous Waste Site Training Course for all
OSCs,  RPMs, and  related personnel.   All  other  field personnel, including
inspectors, are required to take the EPA 24-hour Field Safety Training Course. All

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                                              SECTION: CHAPTER 2
                                              VERSION: FINAL/AUGUST 1993
                                              PAGE: 2-4

training requirements will be in accordance with EPA Order 1440.2.

Since specific  protection cannot be engineered into  every field situation,  it is
essential that employees are trained to implement safe  operational procedures and
are familiar  with the proper use of personal protective clothing and equipment
GLNPO employees and contractors, therefore, must successfully complete the 24-
hour or 40-hour training required and hold certifications attesting that these
requirements  have been  met.   GLNPO employees and contractors are not
permitted to engage in routine field activities until they have been trained and
certified.

No persons,  field EPA employees, or contractors will be permitted to participate in
GLNPO-sponsored activities until they have received appropriate training .


2.1.6 8-Hour Field Health and Safety Annual Refresher Training

After certification  in the 40-hour or 24-hour Field Health and Safety Training
Courses, all GLNPO employees and contractor personnel are required to complete
the 8-hour  Refresher Training  Course on an annual basis.   The  refresher
instruction  is  designed as a review  of subject areas necessary  to  maintain
certification based on the initial 40-hour and 24-hour training.

For further details  on this subject, refer to EPA Order 1440.2 Transmittal July 12,
1981.
2.1.7 First Aid and CPR

EPA employees classified as Chief Scientist and contractor personnel who work on
board GLNPO vessels are required to attend an 8-hour course in First Aid/CPR.
CPR
Courses in Cardiopulmonary Resuscitation (CPR) will be offered throughout the
year. Training may be given by fellow employees who are certified instructors or
by instructors from an outside agency.  Assuming that "someone else" will know
CPR is a dangerous assumption. All  EPA personnel aboard GLNPO vessels are
required to attend an annual 4-hour course in CPR.

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                                               SECTION: CHAPTER 2
                                               VERSION: FINAL/AUGUST 1993
                                               PAGE: 2-5

First Aid
First Aid, like CPR, will be taught throughout the year.  This course is valuable to
all, but  especially important  to  personnel who are engaged  in  potentially
hazardous activities.  Like CPR, knowledge of First Aid  is valuable outside the
work place, as well. All personnel who are required to work on GLNPO research
vessels must attend a 4-hour training session in First Aid, at minimum, once
every three years.
2.1.8 Respiratory Protection

Employees performing tasks requiring the use of respiratory protective devices
must receive training in the use and limitations of a respirator. Personnel must
also be evaluated by a physician to ensure they are medically fit to wear  a
respirator.  If respirators are issued, a written Respiratory Protection Program must
be implemented.  Training and  program requirements can be found in OSHA 29
CFR 1910.134 and EPA Order 14403.

A written Respiratory Protection Program for GLNPO research vessels is described
in Appendix A of this manual. All Emergency Response Personnel aboard the
vessel must meet the requirements specified in the program.
2.1.9 Emergency Response Team Training

Prior to each GLNPO research vessel survey, it is necessary to have a designated
emergency response team. The team must be trained in spill response, respiratory
protection (including SCBA), and emergency response in accordance with OSHA
1910.120. The team members must also be trained in CPR and first aid and conduct
practice drills on an annual basis.

In the event anyone aboard a ship at sea becomes ill or is injured beyond the dear
capability of die ship to provide prompt and adequate care, a Medical Emergency
(ME) exists and mat person should be transferred to a facility providing emergency
medical treatment.

If the Captain (or officer in charge) determines that a possible ME exists, the U.S.
and/or Canadian Coast Guard should be contacted for advice and assistance. The
Coast Guard may be hailed on radio Channel 16 throughout all the Great Lakes.
The Coast Guard can provide medical consultation and evaluation of the situation
and are equipped for evacuation by boat or helicopter, if needed.

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                                               SECTION: CHAPTER 2
                                               VERSION: FINAL/AUGUST 1993
                                               PACE: 2-6
Always contact the Coast Guard for advice. Not all ports have emergency medical
facilities and not all may be suitable for a particular ME.

These procedures  and numbers should be readily available for all employees
aboard GLNPO research vessels.

Telephone numbers for Coast Guard areas and designated trauma centers appear as
Attachments 2.5 of this chapter.
Ship operating personnel must receive training specific to their job functions in
accordance with all regulatory and policy requirements.  Specific training areas
such as fire fighting, heavy equipment operation, and powered industrial truck
operation, are detailed in the following sections.
2.2.1 Fire Fighting

In the event of fire, a well-trained fire brigade must be available to respond
immediately and be accompanied by a qualified chief in command.  All members
of the GLNPO research vessel fire brigade must be trained in accordance with the
U.S. Coast Guard's fire brigade requirements.  Training must include discussions
of fire principles and fire classification, the use of portable fire extinguishers and
proper extinguishing methods, in addition to overall fire safety awareness. Other
qualifications of  the brigade must include  training and experience in fire
protection  and prevention in  addition  to well  established firefighting
organizational capabilities.

Response teams must be available 24 hours a day and regularly scheduled training
for all members of the team must be conducted. Training sessions, at least one
hour  per week, are  recommended.   These  sessions should  include proper
procedures on how to avoid  fire, hands-on training for drills, including signals
and individual responsibilities, as well as procedures for the prevention of bodily
injury. A portion of this training must be devoted to the practice of emergency exit
drills to make certain that all exits are dearly marked and easily passable and that
all alarms are in maximum working order.  Any deficiencies noted during drills or
regular inspections must be corrected immediately.

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                                               SECTION: CHAPTER 2
                                               VERSION: FINAL/AUGUST 1993
                                               PAGE: 2-7

Training documentation must  be made available aboard the vessel and must
include the date of training, name and social security number of the individual
trained, and the name and qualifications of the trainer.
2.12 Cranes

Persons responsible for the operation of cranes, derricks, or hoists on all GLNPO
research vessels are required to receive annual training in the proper use of Ship-
Mounted  Load Handling Equipment.   Training documentation must be made
available aboard the vessel and must include the date of training, name and social
security number of the individual trained, and the name and qualifications of the
trainer.
2.23 Powered Industrial Trucks (Fork Lifts)

The operator is  one of the most  important factors in the safe operation of
industrial or fork-lift trucks.  To ensure operator competence, and mental and
physical fitness,  training is  required.  Initial training must be performed in
accordance with  the National Safety Council Guidelines followed by refresher
courses every two years. Training must include:

      • Operator safety rules
      •  Basic material handling techniques
      •  Basic instruction on the operation of the truck
      •  A driver's performance test on an obstacle course which simulates actual
         driving conditions (e.g. maneuverability)

The operator should be trained to distinguish the differences between a fork-lift
truck and a conventional car or truck. For example:

      •  A lift truck can be steered by either the front or rear wheels
      •  A lift truck steers more easily loaded than empty
      •  A lift truck is often driven as much in reverse as in forward gear

Employees who are assigned to operate powered industrial trucks and/or lift
trucks  should be given a certification  card and should  be required to carry it.
"Rules of Operation" - Safety rules of operation will be given to all industrial truck
and lift truck operators.  These rules are described in Attachment 2.6  of this
chapter.

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                                              SECTION: CHAPTER 2
                                              VERSION: FINAL/AUGUST 1993
                                              PAGE: 2-8
2.3  DOCUMENTATION
Documentation for all EPA required training courses and field certifications must
be maintained by the branch or office safety representative, the GLNPO Safety
Manager, the Branch or Division Safety Representative, the EPA Safety Manager,
and the EPA Training Officer. In all cases, each attendant of an EPA required safety
course must complete a Standard Form 182 prior to training and forward it to the
Regional Training Officer. The information contained in this form will establish a
permanent record and be maintained in the Human Resources Branch Training
Data Management System.

The GLNPO Safety Manager will record and certify that annually required training
courses have been attended by each field inspector or field personnel and issue
proper documentation in the form of a certification card.   The GLNPO Safety
Manager will also be responsible for maintaining annual medical monitoring and
respirator fit testing certification for appropriate field personnel.
The Regional Medical Monitoring Selection Policy is detailed in Regional Order
3130 and is to be followed by all EPA Region V employees to whom it applies. The
purpose of the Regional policy is  to ensure consistency in EPA's approach to
mandatory participation in the Medical Monitoring  Program and to ensure that
EPA  provides the  most comprehensive health  protection possible  for its
employees.  This program is  specifically designed to monitor  the  health of
employees whose work regularly or periodically poses the possibility of exposure
to hazardous materials.

Medical monitoring may consist of a series of blood chemistry tests, urinalysis,
pulmonary function tests, hearing testing,  and chest  x-rays.  Other types of
monitoring mAjj| be necessary based on  the  various  hazards encountered by
individual poANfnel.  A physician  is responsible for identifying and explaining
the significance of all findings. No employee  should leave the health unit with
unanswered questions.  Baseline or preplacement examinations are conducted
prior  to a job assignment where  exposure to  toxic substances  or hazardous
materials may be possible.

Three categories in which Medical Monitoring is mandatory are Hazardous Waste
Site Workers, Laboratory Personnel, and Other  Field Personnel or Inspectors who
are exposed  to toxic chemicals.  Based on this policy, all GLNPO employees who

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                                               SECTION: CHAPTER 2
                                               VERSION: FINAL/AUGUST 1993
                                               PAGE: 2-9

work with potentially toxic chemicals must be monitored for exposure.

The written Medical Monitoring policy is described in Appendix B of this manual.


2.5 ADDITIONAL HEALTH AND SAFETY COURSES

As well as courses that are required for field and laboratory personnel, other
courses  will be offered that are intended for all  employees, including office
workers who do not routinely work in  unsafe  situations.   Courses for  non-
field/lab personnel will be offered that address potentially serious  health risks.
Some courses will be offered consistently throughout the year while others  will be
specially scheduled.


2.5.1 Occupational Noise Exposure

In 1983 OSHA promulgated a hearing conservation amendment to the 1971 noise
standard. This standard defines an effective program and requires that a hearing
conservation program be implemented if workers are exposed to levels equal to or
greater than 85 dB measured on the A scale (slow response).  When employees are
subjected to noise at or  above this PEL, regulations state, "the employer shall
administer an effective hearing conservation program".  Note:  EPA employees
and contractors, including all GLNPO personnel, are required  to wear hearing
protection in all work spaces where noise levels reach or exceed 85 dBA.

The hearing conservation program for GLNPO research facilities is described in
Appendix C of this manual.


2.5.2 Hazard Communication

GLNPO  will ensure  that a Hazard Communication Program is in place  on all
research vessels and that all non-laboratory personnel aboard the vessels are
familiar with the program. The Hazard Communication Program will be based on
the Occupational Safety and Health Administration's regulation stated in 29 CFR
1910.1200. The program will contain a chemical inventory list, material safety data
sheets, proper labeling requirements, and employee  training specifications. The
Hazard  Communication Program for GLNPO is described in Appendix D of this
manual.

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                                              SECTION: CHAPTER 2
                                              VERSION:  FINAL/AUGUST 1993
                                              PAGE: 2-10
2.5.3 Confined Space Entry
The Occupational Safety and Health Act of 1970 lead to the development of the
Safety Training and Education Standard (29 CFR 192621) for confined spaces.  This
standard defines a confined space and requires that employees who enter confined
spaces are trained to recognize  the hazards of a confined space and  know the
precautions to take when entering and working in such a space. Training is also
required for the use of appropriate personal protective and emergency equipment
when working in or around confined spaces.

After review of  fatality and injury data, the Occupational Safety  and Health
Administration found  the existing  standards to be inadequate in protecting
workers from the hazards of confined spaces. In June, 1989, OSHA proposed a new
rule  for the protection of workers in general industry who  work  in confined
spaces.  This rule would require  the development of  a permitting system for
confined space entry, and would address training,  monitoring, and equipment
necessary for entry into confined spaces.  As of April 15,1993, the permit, Required
Confined Spaces Rules, has  become effective  as stated in 29 CFR Part 1910.146,
Occupational Safety and Health Administration (OSHA),  U.S. Department of
Labor.  Further information concerning the Confined Space Entry Program and its
requirements is described in Appendix E of this manual.
2.5.4 Hot Work Procedures

If proper safeguards are not implemented,  activities such as cutting, welding,
heating, grinding or other activities that produce a  flame  or spark have the
potential for creating a fire or explosion. To minimize hazards associated with hot
work, Standard Operating Procedures (SOP) should be developed and followed.

These  procedures should  include measures for protecting  the worker, the
establishment j|f a hot work permitting system, monitoring for chemical exposure,
and monitodpg  for fire  and explosion potential.  When welding, cutting/ or
heating, all personnel and contractors must comply with OSHA 29 CFR 1926.353
for  Ventilation  and  Protection  in Welding,  Cutting, and  Heating  During
Construction Activities.  OSHA 29 CFR 1910.252, General Requirements, must also
be followed.  Additional information outlining Hot Work Procedures is described
in Appendix F of this manual.

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                                              SECTION: CHAPTER 2
                                              VERSION: FINAL/AUGUST 1993
                                              PAGE: 2-11
2.5.5 Lockout/Tagout
The purpose of lockout/tagout is to control the release of potentially hazardous
energy.  The unexpected energization of machines or equipment, or release of
stored energy,  can cause injury or death.  Based on this definition, GLNPO's
Lockout/Tagout  program  is based  on the Occupational  Safety  and Health
Administration's (OSHA) Control of Hazardous Energy Sources Standard found in
29 CFR 1910.147. The program is designed to safeguard the health and well being
of GLNPO and  contractor personnel from a variety of energy sources  when
performing  daily repairs, maintenance,  and  servicing  of their  operating
equipment. To minimize hazards associated with these activities, personnel must
be trained to follow proper lockout/ tagout procedures specified in  the written
program.

The  GLNPO  Lockout/Tagout Program  includes  energy  source surveys,
designations of authorized personnel, affected personnel, training, lockout/ tagout
methods  and procedures,  restoring equipment to  use, and outside contractor
information  exchange.   The GLNPO Lockout/Tagout Program is described in
Appendix G of this manual.
2.5.6 Bloodborne Pathogens

If it is reasonably expected that an employee may be exposed to human blood or
other potentially infectious  materials, employers must protect their employees
from the  hazards of bloodborne  pathogens  and  comply  with the  OSHA
Bloodborne  Pathogen Standard, 29 CFR  1910.1030.   The standard requires
procedures for the use of universal precautions, engineering controls, personal
protective  equipment, proper housekeeping, training, and handling of regulated
waste.  Due to the isolated nature of GLNPO research vessel shipboard operations,
emergency Hrst Aid  response may be necessary  and exposure to potential
bloodborne pathogens may occur.   Based on  this assessment,  a Bloodborne
Pathogen  Program has been developed for  GLNPO research vessels  and is
described in Appendix H of this manual.

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                                   SECTION: CHAPTER 2, ATTACHMENT 2.1
                                   VERSION: FINAL/AUGUST 1993
                                   Alt. 1 -Pagel
Attachment 2.1
                              Training Date:
                              Time: 	
                              Conducted By:
                   24-HOUR LABORATORY SAFETY
                        TRAINING AGENDA
              GREAT LAKES NATIONAL PROGRAM OFFICE
                   RESEARCH VESSEL PERSONNEL

1.    IDENTinCATION OF HAZARDOUS MATERIAL

2.    POTENTIAL SOURCES OF EXPOSURE

3.    TOXICOLOGY

4.    METHODS OF CONTROL

     A. Flow Relationships in Hazardous Materials Laboratories

     B. Ventilation

     C Work Practices (Chemical Handling Protocols, Handling and Labeling)

5.    MONITORING

6.    PERSONAL PROTECTIVE EQUIPMENT

     A. Clothing

     B. EnMMpncy Eyewash & Showers
           . «^^
           -*K
     C. RMppttory Protection

7.    SPILL CONTROL

8.    WASTE AND STORAGE MANAGEMENT

9.    CHEMICAL HYGIENE PLAN

10.   CONTINGENCY PLAN

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                                          SECTION: CHAPTER 2, ATTACHMENT 2.2
                                          VERSION: FINAL/AUGUST 1993
                                          Art. 2 -Pagel
Attachment 2.2 - GENERAL LABORATORY SAFETY RULES
All laboratories and analytical procedures have certain dangers in common.
Almost all analytical  procedures use chemicals  that are considered toxic or
hazardous; examples include acids, bases, and  organic solvents. The samples for
analysis often  contain unknown biological  hazards  or hazardous and toxic
chemicals.  For these reasons, certain safety precautions are necessary throughout
all laboratories  and whenever handling an unknown sample.

Each laboratory and analytical  procedure will have safety rules specified in the
Laboratory Safety Manual or Standard Operating Procedure.  These manuals and
procedures will be specific to each laboratory,  however, the following laboratory
safety rules listed below will apply to all U.S. EPA Region V laboratories:

      1.    All exits and passageways must be  unobstructed and allow free exit.

      2.    Eating, drinking or smoking in the laboratory  is prohibited.

      3.    Appropriate personal protective equipment must be worn for each
            procedure. This includes lab coats, gloves, safety glasses, goggles, and
            occasionally respirators.

      4.    Pipetting by mouth is prohibited.

      5.    All safety signs should be observed and obeyed.

      6.    All toxic waste must be properly disposed according to RCRA
            regulations.

      7.    Flammable, toxic or radioactive material must  be stored in approved
            containers.

      8.    All containers of chemicals and samples shall be labeled dearly and
            correctly.

      9.    All gas cylinders must be firmly secured to prevent falling.

      10.    All electrical equipment should be properly grounded and electrical
            cords inspected periodically to ensure that they are in good working
            condition.

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                                         SECTION: CHAPTER 2, ATTACHMENT 22
                                         VERSION: FINAL/AUGUST 1993
                                         Att. 2 - Page 2

      11.    Floors and surface working areas are to be kept dean, dry and free
            from corrosive chemicals.  Spills must be cleaned up immediately.

      12.    Initiation of a laboratory analysis or experiment, not fully described in
            Standard Methods, AQC Manual or other procedural source, must be
            preceded by a survey of existing literature. The toxirity and other
            hazards connected with the reactants and products should be
            investigated thoroughly before starting any laboratory reaction.
CONTINGENCY PLANS

Contingency plans must be developed and implemented in cases of accidents or
emergencies.  A spill contingency plan is necessary when toxic or  hazardous
substances are on site.  Personnel must be trained in spill cleanup procedures.
Depending on the size of the laboratory and the operations performed in the
laboratory, a special team may be needed for emergencies. At smaller installations,
the local  fire department or emergency response team may be contacted for
emergencies.  Emergency response teams will require training in respiratory
protection, first aid, and spill cleanup.  The  duties of the team  must be clearly
denned. Evacuation plans should be distributed, posted and updated yearly.
VENTILATION
Proper ventilation must be provided for analytical procedures and especially those
involving the use of toxic substances.  Most laboratories will be equipped with
fume hoods to provide proper ventilation.  Fume hoods must be tested semi-
annually to assure  proper flow rates  and containment.  Maintenance must be
conducted on a regular basis to insure proper functioning. Exhaust air from glove
boxes must be treated by filtration, reaction, absorption, adsorption, electrostatic
precipitation or incineration depending on the chemical compounds being used.
All exhaust air from primary containment equipment must be discharged by roof-
mounted blowers and dispersed clear of occupied buildings and air intakes.
Additionally, a mechanical exhaust ventilation system must be in place to control
laboratory room air movement.  The air supply must originate from areas where
the potential for contamination is low, and exhaust into outside areas.

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                                         SECTION: CHAPTER 2, ATTACHMENT 2.2
                                         VERSION: FINAL/AUGUST 1993
                                         Att.2 -Page3
STORAGE REQUIREMENTS
Proper storage techniques must be followed and are  detailed in  the following
sections.
STORAGE OF COMPRESSED GAS CYLINDERS

Only compressed gas cylinders required for immediate use are allowed to be stored
in laboratory work areas.  Separate areas should be provided for storage of all other
gas cylinders.  Empty and full cylinders should  not be stored  together.  Oxygen
cylinders must  not be  stored near  flammable gas cylinders.  Cylinders  of
compressed  gases must be stored with the valve end up and securely fastened to a
wall or immovable object to prevent the cylinder  from falling. All cylinders must
be securely fastened (strapped  or chained) to prevent falls.  All  cylinders must  be
kept away from heat sources.
STORAGE OF CHEMICALS

All chemicals or waste materials in the  laboratory, stockroom, or bulk storage
must be stored according to their chemical compatibility.  Storage  control will
minimize the potential for accidental mixing by spillage, breakage, or fire.  Major
storage areas are required for  the  following  types  of chemical  compounds:
flammable solvents, corrosive liquids, and strong oxidizers.  Additional storage
may include areas for radioactive materials, biological hazards,  highly toxic
materials and compressed gases.

When  working  in laboratory areas,  only the  minimum amount of chemical
needed for  analysis  operations should  be stored.   Separate storage areas
(stockrooms) should be provided for the  main bulk of chemicals. Incompatible
chemicals should either be stored in separate safety storage cabinets, divided by a
wall, or enclosed by a  tray to prevent accidental mixture.  These storage areas and
containers should always be properly labeled.

The main  storage areas should be divided  to accommodate incompatible
chemicals.  Depending on the amount of chemicals in storage, approved safety
storage cabinets can be used. If necessary, storage rooms can also be used.

No more than five one-liter containers of Class I and n  materials should be stored
in open laboratory areas. Open laboratory  areas include reagent shelves and bench

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                                         SECTION: CHAPTER 2, ATTACHMENT 2.2
                                         VERSION: FINAL/AUGUST 1993
                                         Att.2 -Page4

tops.  All glass containers must be protected by an outer sheath or made of
unbreakable glass.

Storage areas for waste collection should be provided and properly designated.
Provisions for containment and separation of incompatible waste materials are
also required.

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                                      SECTION: CHAPTER 2, ATTACHMENT 2.3
                                      VERSION: FINAL/AUGUST 1993
                                      Att.3 -Pagel
Attachment 2.3
                                Training Date:
                                Time:
                                Conducted By:
                     4-HOUR LABORATORY SAFETY
                          TRAINING AGENDA
               GREAT LAKES NATIONAL PROGRAM OFFICE
                     RESEARCH VESSEL PERSONNEL
1.     CHEMICAL HEALTH AND SAFETY RESPONSIBILITIES

      A.    Federal Safety and Health Regulations

      B.    EPA Occupational Health and Safety Program

      C    OSHA 1910.1450 Occupational Exposures to Hazardous Chemicals in
           the Laboratories.

2.     RIGHT TO KNOW

      A.    Chemical Inventory

      B.    Hazard Identification

      C    MSDS

      D.    Labeling

      E    Twining

3.     CONTROLS

      A.    Chemical Handling and Storage

           1.    Flammable Materials
           2.    Corrosives
           3.    Highly Toxic Chemicals

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                                   SECTION: CHAPTER 2, ATTACHMENTS    ™
                                   VERSION: FINAL/AUGUST 1993
                                   Alt.3 -Page2
     B.    Ventilation

     C    Personal Protective Equipment

4.    SOPs/SAFETY PLANS/EMERGENCY PROCEDURES

5.    SPILL CONTROL/HAZARDOUS WASTE
                                                                   i

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                                       SECTION: CHAPTER 2, ATTACHMENT 2.4
                                       VERSION: FINAL/AUGUST 1993
                                       Att.4 -Pagel
ATTACHMENT 2.4
                                  Training Date:
                                  Time:
                                  Conducted By:
                          TRAINING AGENDA
                                  FOR
               GREAT LAKES NATIONAL PROGRAM OFFICE
                      RESEARCH VESSEL PERSONNEL
I.     Subject

      1. GLNPO Chemical Hygiene Plan

      -  general overview
      -  use of PPE
      -  health and safety SOPs for analytical procedures
      -  MSDS  overview

      2. Environmental and Health and Safety Procedures

      -  hazmat/waste transportation
      -  hazmat storage/spill prevention
      -  hazardous/common waste
      -  contingency plan (plan, SCBA, APR, spill cart, instruments, fire,
        identify team members)

      3. General

      -  MSDS review for specific materials
      -  safety committee
      -  lockout/tagout awareness
      -  general comments

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                                        SECTION: CHAPTER 2, ATTACHMENT 2.4
                                        VERSION: FINAL/AUGUST 1993
                                        Att.4 -Page2

IL    Participants

      Name - Print                   Name - Sign               Date

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                                                  SECTION:  CHAPTER 2, ATTACHMENT 2.5
                                                  VERSION:  AUGUST/FINAL 1993
                                                  Art. 5 - Page 1
Attachment 2.5
                                    MARINE MEDICAL

                              EMERGENCY OPERATIONS PLAN

                                U. S. C G. Group: Great Lakes

In the event anyone aboard a ship at sea becomes ill or is injured beyond the clear capability of the
ship to provide prompt and adequate care, a Medical Emergency (ME) exists and that person should
be transferred to a facility providing emergency medical treatment.

If the Captain (or officer in charge) determines that a possible ME exists, the US. and/or Canadian
Coast Guards should be contacted for advice  and assistance. The Coast Guards may be hailed on radio
Channel 16 throughout all the Great Lakes.  The Coast Guards can provide medical consultation and
evaluation of the situation and are equipped for evacuation by boat or helicopter, if needed.

Always contact the Coast Guard for advice.  Not all ports have emergency medical facilities and not
all may be suitable for a particular ME. These procedures and telephone numbers should be readily
available to all employees aboard our research vessels.
2.5.1 Coast Guard
Buffalo
Lake Ontario & East 1/2 of Lake Erie

Coast Guard: (716)846-4152
Health Services:  (716) 846-4167
Trauma Centers:  Mercy Hospital, Buffalo, NY
               (716) 828-2790
               Sisters of Charity, Buffalo, NY
               (716) 862-2840
Detroit
West 1/2 of Late Erie and Lower 2/3 of Lake Huron

Coast Guard; (313)568-9524
Health Services:  (313)568-9526
Trauma Centers:  Detroit Receiving, Detroit, MI
               (313) 745-3349
               Henry Ford, Detroit, MI
               (313) 641-4029
Sault St Marie
Lake Superior and Upper 1/3 of Lake Huron
       and Upper 1/3 of Lake Michigan

Coast Guard:  (906)635-3233
Health Services:  (906)635-3225
Trauma Centers: Marquette Gen., Marquette,
               MI
(800)562-9753

Northern Michigan Regional, Petosky, MI
(616)348-4000

Grand Haven
Eastern 1/2 of Lake Michigan

Coast Guard:  (616)847^501
Health Services:  (616)847-4542
Trauma Centers: Ottawa County Hospital
               (616)847-5310

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Milwaukee
Western 1 /2 of Lake Michigan
Coast Guard:  (414)747-7181
Health Services:  (414)747-7111
Trauma Centers: Froederct General Hospital
               (414)259-3000
                                                SECTION:  CHAPTER 2, ATTACHMENT
                                                VERSION:  AUGUST/FINAL 1993
                                                Art. 5 - Page 2
            k     i°nal
ILLINOIS
Chicago

Northwestern Memorial Hospital
Superior St. & Fairbanks Court
Chicago, IL 60611
(312)908-2000
Ask for Emergency Rm.; 5-10 minutes from harbor.
Columbus Hospital
2520 N. Lakeview Avenue
Chicago, IL 60614
(312)883-7300
5*10 minutes from Lake; unsure of distance
       from harbor.
St. Margaret Hospital
5454 Hohman Avenue
Hammond, IN  46320
(219)932-2300

Charlevoix

Charlevoix
Lakeshore
Charievoix. Ml
(616)547-0027
1 mile from harbot

Frankfort

Paul Oliver Memorial Hospital
224 Park Avenue
Frankfort, MI 49635
(616)352-9621
Half mile from harbor.
Zion

American International
Emrnaus & Shiloh Boulevard
Zion,IL 60099
(708)872^1561
Unknown distance from harbor. There is more
       than one in area

INDIANA
Gary

Methodist Hospitals Northwest Indiana
600 Grant Street
Gary, IN 46402
(219)886-4000
20 miles from harbor; 15 minutes.

MICHIGAN
Arcadia (See Frankfort)
Escanaba

St. Francis Hospital
3401 Ludington Street
Escanaba, MI 49829
(906)786-3311
2 miles from harbor.

Grand Haven

N. Ottawa Community Hospital
1309 Sheldon Road
Grand Haven, MI 49417
(616)847-5310
2 blocks from harbor.

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Harbor Springs (See Petoskey)

Leland (See Frankfort or Traverse City)

Lading tun

Memorial Medical Center
One Atkinson Drive
Ludington, MI 49431
(616)845-2390
1 mile from harbor.

Manistique

Schooicraft Memorial Hospital
500 Main Street
Manistique, MI 49854
(906)341-2163
3 blocks from harbor.

Pentwater (See Ludington)

Petosky

Northern Michigan Hospital
416 Connable Street
Petosky, MI 49770
(616)348-4520
10 miles from harbor.

Portage Lake (See Manistee)

Traverse  City

Munson Medical Center
1105 Sixth Street
Traverse  City, MI 49684
(616)922-9000
5 minutes from bay

MINNESOTA.
Duluth

St. Mary's Emergency Care Trauma Center
407 E. Third Street
Duluth, MN  55805
(218)726-4353
 SECTION: CHAPTER 2, ATTACHMENT 2.5
 VERSION: AUGUST/FINAL 1993
 Art. 5 - Page 3

 Holland

 Holland Community Hospital
 602 Michigan Avenue
 Holland, MI 49423
 (616)394-3202
 10-15 minutes from harbor.

 Manistee

 West Shore Hospital
 1465 E. Parkdale
 Manistee, MI 49660
 (616)723-3501 Ext 150
 5 miles from harbor.

 Montagu e/Whitehall/Muskegon

 Hackley Hospital
 1700 Clinton Street
 Muskegon, MI 49443
 (616)726-3511
 3 miles from harbor.

 Mercy  Hospital
 1700 Oak Avenue
 Muskegon, MI 49442
 (616)739-3916
 10 miles from harbor.

 South Haven

 South Haven Community Hospital
955 S. Bailey Avenue
South Haven, MI 49090
 (616)637-5271

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WISCONSIN
Gill's Rock ( See Sturgeon Bay)

Kenosha

Kenosha Hospital
6308 Eighth Avenue
Kenosha, WI 53140
(414)656-2201
5-8 blocks from harbor.

Manitowoc

Holy Family Medical Center
2300 Western Avenue
Manitowoc, WI 54221
(414)684-2011
21 blocks from Lake.

Port Washington

St Mary's Ozaukee
743 N. Montgomery Street
Port Washington, WI 53074
(414)284-7911

Sheboygan

Sheboygan Memorial Medical
2629 N. 7th Street
Sheboygan, WI 53083
(414)459-5553
5 minutes or less from harbor.

St Nicholas Hospital
1601 N. Taylor Drire
Sheboygan, WI 53081
(414M59-830Q
3-4 min. from harbor

SUPERIOR
Sturgeon Bay

Door County Memorial
330 S. 16th Place
Sturgeon Bay, WI 54235
(414)743-5566
5-10 miles from harbor.
                                                 SECTION: CHAPTER 2, ATTACHMENT 2.5
                                                 VERSION: AUGUST/FINAL 1993
                                                 Art. 5 - Page 4
 Green Bay

 St. Mary's Medical Center
 1726 Shawano Avenue
 Green Bay, WI 54303
 (414)498-4560
 1 hour from bay.

 Kewaunee

 St Mary's Memorial
 First 6t Lincoln
 Kewaunee, WI 54216
 (414)388-2210
 5 minutes or less from harbor; about 2 miles

 Milwaukee

 St Luke's Medical Center
 2900 W. Oklahoma Avenue
 Milwaukee, WI  53215
 (414)649-6000

 Racine

 St. Mary's Medical Center
 3801 Spring Street
 Racine, WI 53405
 (414)636-4201
 5-10 minutes from harbor.

 St. Luke's Memorial
 1320 Wisconsin Avenue
 Racine, WI 53403
 (414)636-2201
 5-10 minutes from harbor.
Two Rivers

Two Rivers Community Hospital &
       Hamilton Memorial Home
2500 Garfield Street
Two Rivers, WI 54241
(414)793-1178 Ext 125
6 blocks from Coast Guard Station

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                                               SECTION: CHAPTER 2, ATTACHMENT 2.5
                                               VERSION: AUGUST/FINAL 1993
                                               Att. 5 - Page 5
CANADA
Thunder Bay

Port Arthur General Hospital
460 N. Court St
Thunder Bay, Ontario P7A4X6
(807)343-6621
5-10 minutes from Marina
Welland Canal

Welland Co. General Hospital
Third Street
Welland, Ontario L3B4W6
(416)732-6111
30 minutes from Lake Ontario
•NOTE: All hospitals listed provide 24-hour Emergency Room Service with a physician on duty.

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                                        SECTION:  CHAPTER 2, ATTACHMENT 2.6
                                        VERSION:  FINAL/AUGUST 1993
                                        Att. 6 - Page 1
Attachment 2.6 POWERED INDUSTRIAL TRUCKS (FORK LIFTS)
                          RULES OF OPERATION

Safe rules of operation must be followed by all powered industrial truck drivers.
The drivers should keep the following rules conveniently located, refer to them
frequently, and  use them  as  a checklist for safety.  These rules have  been
developed in accordance with ANSI-B56.1-1983 (Revised).

    1. At the beginning of each use, check brakes, steering controls, forks, hoists,
       warning devices and lights.  Report any defects to the supervisor
       immediately. Also check to see that the fire extinguisher is in place and
       properly serviced.

    2. Always face the direction of travel.

    3. Before you start driving, look to see that no person or object is in your
       path. Always look before backing up.

    4. Do not drive over objects lying on the floor such as trash, lumber and
       pipes. These could damage the truck, shift or topple the load, or flip out
       and strike another employee.

    5. Use low gear when going down ramps or steep  grades. On upgrades, keep
       the load in front. On downgrades, keep the load in back.

    6. Under normal traffic conditions, keep to the right.

    7. Avoid quick starts or turns, and jerky stops; always come to a complete
       stop before reversing the direction of travel.

    8. Keep a safe distance between vehicles at all times (three truck lengths).

    9. Make a complete stop at all doors, comers, exits and stop signs.

    10. No horseplay at any time!

    11. Keep alert—concentrate on the job at hand.

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                                    SECTION: CHAPTER 2, ATTACHMENT 2.6
                                    VERSION: FINAL/AUGUST 1993
                                    Att. 6 - Page 2

12. Remember that pedestrians have the right of way at all times.

13. Never drive with wet or greasy hands. If necessary, keep a towel or rag
    handy at all times.

14. Reduce speed on wet and slippery floors.

15. Whether loaded or empty, cany forks and platforms on lift trucks as low
    as possible.  This lowers the center of gravity and reduces the possibility of
    overturning the truck or dumping the load.

16. Don't run trucks onto elevators unless authorized to do so. Be sure you
    have sufficient clearance before entering any elevator or going through a
    doorway.  Once the truck is in the elevator, shut off the power, set the
    brakes and stay in the operator's seat in case of an emergency.

17. Check the clearance of any overhead crane or other object before raising
    forks or tiering.

18. Never use your truck as an elevator for other employees (for example, to
    service light fixtures or stock material).

19. When leaving the truck at any time, shut off the power, set the brakes,
    remove the ignition key, and put the forks or platforms in the down
    position.

20. Never leave a truck in an aisle. Park the truck to one side, so traffic will
    not be blocked.

21. Before driving the truck into a freight car or trailer, always secure the
    bridging plate with bolts or pins so that it cannot move when the power
    wheels of the truck pass over it; and before moving the truck onto the
    bridge or into the carrier, walk into the freight car, truck or trailer and
    check the floor for holes or weak spots  (the weight of your truck might
    break the floor).

22. Keep your feet and legs inside the guard, which is there to protect you.

23. Drive with extreme care, especially during shift changes.

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USEPA R/V MUDPUPPY
    APPENDIX B

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 APPENDIX B:  R/V  MUDPUPPY  - HEALTH & SAFETY PROCEDURES
                   DURING SEDIMENT  SAMPLING

INTRODUCTIOW

This Appendix has been developed in accordance with Chapter 10, and
Appendix   0,   of  the  GLNPO  Health,   Safety  and  Environmental
Compliance Manual.   Applicable portions  of the GLNPO  Manual have
been extracted  and incorporated  into  this appendix.   Additionally,
relative  information specifically  developed for safety  aboard the
R/V Mudpuppy has been incorporated.

BACKGROUND

The Sediment Assessment Program  is  designed to sample and evaluate
areas of  sediment  where  the possibility  of  contamination  exists.
During  sampling  activities,   field   specialists   and  laboratory
analysts may be  exposed to sediment which represents  a chemical  or
biological hazard.   Therefore,   the  potential  for exposure  should
always be of foremost concern when handling  sediments  of  unknown
contamination.

The Great  Lakes National  Program Office  (GLNPO) sediment  sampling
activities are grouped into the  following five  categories:

     1.    Sediment sampling at "Areas Of Concern"  (AOC)

     2 .    Tributary  sediment monitoring

     3 .    Open  lake  sediment monitoring

     4.    Support  for EPA  regions  for  Superfund  and  enforcement
           activities

     5.    Miscellaneous activities

Sediment  assessment  and tributary monitoring may entail  collecting
long  core samples  with low  to high  amounts  of  contamination.
Typical AOC assessment span six  to  twenty days. Open lake  sediment
monitoring is generally conducted aboard the R/V Lake  Guardian.

Sediment   Muqpling   to  support  EPA   regional  offices  or   other
governmentail agencies provides  the  largest degree  of diversity in
sampling activities.   Sampling operations range from  the  use of all
or  part  of the  R/V  Mudpuppy  equipment to  field-flexible  sampling
plans based  on  observations of samples  and/or  their environments.
Most  of  GLNPO's sediment  sampling  activities,  however,  will be
performed  on board the R/V Mudpuppy  either on an outdoor  work  bench
or  in a mobile  laboratory.

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SAFETY

General

Prior to commencing  field operations,  the field team  should gather
as much information  as  possible on the on the site data  and assess
the  sediment hazard potential.    If  hazard  is  un-know the  site
should be treated as potentially hazardous.

Appendix B  of this  manual  (R/V  Mudpuppy Health  &  Safety  Manual)
contains information regarding  the proper  selection  and uses  of
Personal Protective  Equipment  (PPE) during sediment sampling aboard
the  R/V  Mudpuppy.    Additionally,  Appendix  B  contains   proper
procedures for the donning and doffing of PPE.

Facility Engineering Protection

The  following guidelines should  be  implemented during sediment
assessment to decrease the potential of cross contamination  and  the
tracking of potentially hazardous materials:

     Housekeeping  -  Good  housekeeping  practices  are  essential,  not
     only  from  a  general  safety  stand point,  but  also  from a
     contamination reduction stand point.  Potentially contaminated
     materials are not allowed within the cabin area.

     Work  Surfaces   -  Following  sampling   activities  all  work
     surfaces shall  be decontaminated to the extent possible.

     Clean-up  of  Equipment  -  Following  sampling activities,   all
     sampling  equipment  shall  be decontaminated  to  the  extent
     possible.

     Sediment  Storage -  Sediments samples  must  be  stored  inside
     capped core tubes or inside sample containers.

Sediment Assessment/Field Operations Safety Rules

The  following  rules  must  be followed by  sediment  assessment fiel •.
personnel:

     1.   Mb eating,  drinking  or  smoking  is  permitted  durir. :
          Mdiment sampling.

     2.   When  away  from the  dock,  a  Personal Flotation  Devir-
           (PFD)  must be worn anytime you are out of the cabin.

     3 .   No  one is to go  onto the cabin roof  without permissi •
          from the Captain.

     4.   No  one is  to climb onto, stand up on, or move  around
          top of the cabin roof while che vessel is underway.

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     5.    While underway, no one  is  to  move to the aft deck by way
          of the outboard edge and handrail.
     6.    While underway, t>ry to keep movement to a minimum.
     7.    When  lifting equipment  with  the boom  and  winch,  hard
          hats,  work  vest,  and  steel  toed  shoes /boots  must  be
          worn .

     8.    When   collecting  sediment   samples,   only  designated
          collection crew are to be on the  forward  deck.

     9.    When collecting  sediment, PPE  must be worn in accordance
          with Appendix
     10 .   No contaminated  PPE is  to be worn  or carried  into the
          cabin area.

     11.   When sediment  is  present on the forward deck, no one is
          to  step  out  onto  the  deck  unless  the  minimum  PPE
          requirements have been met.
               r\
     12.   Heat-// related  emergencies  are  of  primary  concern  on
          board^ the  R/V Mudpuppy.   Good judgement is  essential.
          Pace yourself  and know  your  limits.   During extreme heat
          the  Captain will be  monitoring  your  condition.    It  is
          recommended that you drink plenty  of  water  (not  while in
          a contaminated suit)  and take several breaks  if needed.
            If  you  feel the  effects  of heat  stress,  inform  the
          Captain.

     13 .   The  Captain is in charge  of  the vessel and  the overall
          safety of the vessel.  Obey the Captain's orders.

     14.   Do   not   stand  under  pieces  of  equipment  that  are
          suspended.

     15 .   Do not stand on cables or ropes .

     16.   A single layer of gloves must  be worn when  on top of the
          cabin.
     17.  D»  not  sit  on  air  cor.ditioning  unit  on  top of  cabin
          roof.

     18.  Use common sense at all times.
HEALTH AND SAFETY PROTOCOL FOR EPA VESSELS

This section contains applicable health and safety requirements,  as
stated in Appendix 0 of the GLNPO Health,  Safety,  and Environmental
Compliance Manual,  "Health & Safety Protocol For EPA Vessels".

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General Provisions

EPA vessels  are  public vessels of  the  United States.   The  Code of
Federal Regulations  (CFR)  exempts public  vessels  from many  of  the
regulations  specified for  certification of  commercial shipping.
However,  it  is   the  intent  of  EPA to  maintain  compliance  with
standards  of  marine  safety   as  set   forth  by   international
convention,  U.S.  Law,  the Code  of Federal Regulations,  regulatory
bodies, U.S. Coast Guard guidance,   accepted  industry  standards and
codes  satisfactory  for maritime use.   This protocol  (this  section
[Health & Safety  Protocol For  EPA Vessels])  describes  essential
requirements  that must be met to attain these overall  marine health
and safety objectives.

All  aspects  of   "good  marine  practice"  cannot  be  set  forth  in
statutes,   regulations,  or  standards.    The  absence  of  a  written
protocol  for a specific issue  should not  be interpreted that  the
issue has a  diminished importance  or priority.  Ship Masters  should
emphasize  to all  personnel on  board  an  EPA Vessel  that  general
health and safety be considered at all  times.

Implementation

It  is EPA's intent  to  modify,  as appropriate,   vessel  operating
contracts  and  Health  &   Safety  Manuals   to   incorporate   the
requirements  outlined in  this  protocol.   Unless  otherwise noted
herein, it  is the responsibility  of the  Ship  Master  to  implement
and comply with the requirements of this protocol.

Medical Mon,itQring/Recordkeeping

All medical  monitoring and recordkeeping for personnel on board  the
R/V Mudpuppy must be conducted  in accordance with  Chapter  Two of
the GLNPO Health,  Safety and Environmental Conpliance Manual.   It
is  necessary to maintain,  on  board the vessel,  certification  and
medical clearance documentation for all personnel  conducting work
activities  which fall  under the  provisions  set  forth in  29   CFR
1910.120  (HAZWOPER).  Annual physical examinations are required  for
all crew members on board EPA vessels.

Divers     ^

Since  the Wff Mudpuppy does not anticipate being  involved  in  any
diving activities, protocol  for divers/diving will  not be included
in  this document.   Should  the  situation change, refer  to the GLNPO
Health,  Safety,   and Environmental  Compliance Manual  for  Diving
Protocol.

Training  Requirements

Fist  Aid  and  CPR  -  All  licensed  crew  members   and EPA  Chief
Scientists shall maintain current  First Aid and CPR certifications

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Pire Fighting  -  At least one crew member on board the R/V Mudpuppy
shall attend and successfully complete  a  Fire  Fighting course.


Medications and  Medical  Supplies

A  sufficient  supply of  medications and  medical supplies  shall  be
available  on  board the  R/V Mudpuppy  for  the  treatment of  non-
emergency  sicknesses and  injuries.   Supplies  must be  kept  in  a
convenient location and  be readily available  for  use.   The medical
cabinet  must  be   inventoried  and  restocked  monthly.     Expired
medications  shall  be  disposed of  immediately.    A  list  of  the
contents,  and  any  necessary instructions,  shall  be posted  inside
the  medical  cabinet.   It  is  the Ship  Masters responsibility for
replenishing   stocks,    discard   expired  medications,    updating
listings,  posting  use  instructions  and  keeping  the cabinet  well
organized.

Medical Emergencies

In  the  case   of  personal  illness or  injury  deemed  sufficiently
serious  by the  Ship Master to  warrant treatment  beyond  the first
aid  abilities  on  board,  the following  course of  action shall be
implemented:

     1.    Survey  equipment in  the  water  shall   be  immediately
           recovered and  the vessel shall make for  the nearest  pert
           at the highest speed  possible,  consistent with  safety.

     2.    Radio  contact  with the  port of arrival shall be made and
           dockside clearance with  an ambulance  standing by shall
           be requested.

The  Ship  Master  is responsible for maintaining  a  directory of all
USCG Stations, with applicable  telephone  numbers.   The Ship Master-
must be  aware of  the  nearest  medical  facility in  respect  to  t.-.-
vessel's operational area.

Reporting Medical  Emergencies

In  accordance with OSHA  requirements,  following  any  applicahl •
injury on board, EPA personnel shall complete Form CA-1,  "Feder-;.
Employee's BJotice  of Traumatic  Injury and Claim  for Continuation   :
Pay/Compensation".   This  form  shall  be filed with the  employee
supervisor  for appropriate action    Additionally,  any accident -
any  person on board which  results  in  property damage,  injury
death,  shall  be reported  immediately to the  appropriate EPA  Sh.
Project Officer  by either  the injured party,  the Chief Scientist
the  Ship Master.   A written confirr.ation of  the  accident must
submitted  within 48 hours  after an accident occurs.   Such repcr-
shall  include  full details of  the accident,  including witr.-
statements.  The Ship Contractor  shall  provide a written report

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the EPA Project  Officer on any claim made by  a  third party against
the Ship Contractor.

Survey Personnel Reporting On Board.

A  sign-in sheet  should be  completed by  each  person arriving  on
board the R/V Mudpuppy.  The sheet shall be  maintained by the Chief
Scientist or  the Ship Master for the  duration of the  survey.   The
sheet shall contain,  at a minimum, the following information:

     1.   Date(s)  of  Survey
     2.   Name and Address
     3 .   Telephone Number
     4 .   Emergency Contact  Person

Orientation

Each person  reporting  on  board the  R/V Mudpuppy  shall  receive  a
shipboard  orientation  prior  to  sailing.    The  orientation  shall
include:

     1.   Review of the R/V Mudpuppy  Health  &  Safety Manual
     2.   Review of the R/V Mudpuppy  Health  &  Safety Rules
     3 .   Requirements  & Proper Use of PPE
     4.   Location of Emergency Equipment
     5.   Emergency Response Procedures
     6.   Review of Applicable MSDSs

Safety Equipment &  Procedures

In  addition  to  the  Personal  Protective Equipment  (PPE)   required
under Appendix B of the R/V Mudpuppy Health & Safety Manual,   the
following safety equipment is required on board:

     Fire Extingui»h«r» - It is recommended, that a minimum  of three
     fire  extinguishers  must  be  maintained on  board   the  R,V
     Mudpuppy.   Monthly inspections must be  informed to ensure the
     extinguishers  are  properly  charged  and  ready  for  use.
     Additionally,  the  extinguishers  must  be inspected annually by
     a certified outside contractor.

     Lif« 3kcJt«t« - The vessel shall  be  equipped with  life jackets
     for  afr least 110 percent  of  the  vessel's maximum  complement.
     Life jackets must  be  readily accessible and clearly stencile^
     with the vessel's  name.  All life vests must be US Coast Guar ::
      (USCG)  Approved  and meet  the  working  .standard as  set  forth c-,
     USCG regulations.

     Ring Life Buoys  -  Ring  buoys  shall  be distributed so  they ar-
     readily available  on both sides  of the vessel.  All unit sha . .
     be  stored so they  are  capable  of being  rapidly cast loose a;
     not  permanently  secured in any way.

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     Work  V«at» -  Work vest  shall be  available and  used by  all
     persons involved  in deck  operations.

     Distress Signals  - A minimum of  twelve  approved,  in-date,  hand
     held,  and/or  rocket-propelled  red parachute  flare  distress
     signals  shall be  carried on  board.    These distress  signals
     shall  be  stored  in  the  cabin  area and  shall  be kept  easily
     accessible  for immediate  use.

Safety Inspections/Tests

It  is  the responsibility   of the  ship's  contractor  to  conduct
inspections  and  tests,  and  to  document   the   results   of   such
inspections/tests.  The following  is a list  of required inspections
and tests to be conducted on board the R/V Mudpuppy:

     Emergency  Power  Systems  -  Emergency power  systems  are to  be
     inspected/tested  on a weekly  basis.

     Emergency   Eye   Wash   -   Emergency   eye   wash   must    be
     inspected/tested  on a weekly  basis

     Fire Extinguishers - Fire extinguishers must be inspected on a
     weekly  basis.    Additionally,  annual   inspections  must   be
     conducted  by a certified  outside contractor.

     Emergency  Radio  - Prior  to  each survey,  the emergency radi:
     must be tested to ensure  it  is in proper  working order.

     Lifesaving devices - On  an  annual  basis, all  life rings ar.-:
     PFDs must  be inventories  and  inspected.

     Load  Handling Gear  -  Visual inspections  must  be   conducts •.
     prior  to  each use.  Additionally,  annual inspections must  c-
     conducted  by a certified  outside contractor  (mu'st  include 1: ~- :
     testing).

     First  Aid Equipment  -  must  be  inventoried  and  restocked  i-
      least monthly.

Also required are any  inspections mandated by  the USCG  or  the GU'7
Health,  Safety  and Environmental  Compliance Manual  that  do  r  -
appear in this  listing.

Hazardous Or Chemical Waste

Any hazardous waste generated  on beard shall be stored and dispc^--
of in accordance with  applicable regulations.  EPA Chief Scienti.-
shall be responsible  for ensuring proper  storage  and  disposal -
hazardous waste.  All  waste  containers must be properly labeled
include  the chemical content and the accumulation start date.

Annual and Periodic Safety Audits

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                      — -~i  	  	  —  	 --  -- =	
Managers or  their  delegated officials in accordance with  the  GLNPO
Health, Safety and Environmental Compliance Manual.

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USEPA R/V MUDPUPPY
    APPENDIX C

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     APPENDIX C:  R/V MUDPUPPY -  PERSONAL  PROTECTIVE
              EQUIPMENT  POLICIES  & PROCEDURES
INTRODUCTION

This appendix has  been developed in accordance with  Appendix I of
Che  GLNPO  Health,   Safety,  and  Environmental  Compliance  Manual.
Applicable portions  of the  GLNPO manual  have been  extracted and
included as part of  the R/V Mudpuppy Personal  Protective Equipment
(PPE)  requirements  and procedures.   Additionally, other relevant
PPE  requirements  and  procedures  have  been  developed  and  are
included in this appendix.

POLICY

It   is  the  policy  of  the  Environmental  Protection  Agency  to
administer its programs in a manner that will assure the protection
of  the  health  and  safety  of all persons  involved  in  routine field
and  laboratory work, especially  those persons  engaged in work of a
hazardous nature,  by providing  and requiring  the  use of specified
personal protective clothing and equipment.

REFERENCES

     1.   Occupational  Safety and Health Act, P.L.  91-596

     2.   29  CFR  1910,  Subpart  I.  Sections  132,   133,   134,  135,   ™
          136,  137  and  139

     3.   EPA  Order  1440.2  Health and  Safety  Requirements  for
          Employees  engaged in  field activities,   EPA Order  1440.1
          Respiratory  Protection

     4.   GLNPO  Health Safety and  Environmental Compliance  Manila.
          Appendix I

DEFINITIONS /RESPONSIBILITIES

Applicable terms and responsibilities are defined as follows:

Field
 The  term {Said activities, means  EPA program activities  that  ar-
 conducted" by EPA  enployees outside D£ EPA administered facilities
  These activities include, but  are not  limited  to, 'environment^
 and  pesticide sampling,  field analysis,   inspection  of water  ar
 waste  water treatment plants,  hazardous material spills and  was~-
 site investigations,  inspections, and sampling.

 Atrolicabilitv

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The  provisions  of   this   section  are  applicable  to  all  GLNPO
employees at  all operational levels .   They are also  applicable to
EPA contractors  and other governmental  agencies.
Regional
The  Regional  Administrator  is responsible  for  implementing  the
regional safety program of which  the requirements of  this  section
are a part.  It  is  the  responsibility of the  Regional Administrator
to  require  Division  and  Office  Directors  to  budget  the  funds
necessary to procure Personal  Protective Equipment  (PPE) .

Division & Office Directors

The Division and Officer  Directors  are responsible for  assigning
staff to field work which may  require the use of PPE.

Supervisors

In accordance with the procedures of  this manual, the supervisor  is
responsible  for determining  the  level  of  protection required  (in
consultation  with  the  Health  &  Safety  Officer) .    He/she   is
responsible  for  controlling,  issuing,  and  inspecting  the  PPE
selected.    He/she  is  responsible for  identifying  employees  who
require  keeping an  accurate inventory and  for  maintaining the PPE
in a  functional condition.   He/she is responsible  for identifying
employees who require training and certification in accordance with
the  provisions  of   EPA Orders  1440.2,  1440.3  and OSHA 29  CFR
1910.120;  and  for  ensuring  these requirements  are contained   in
their position description.  He/she is responsible for recommending
appropriate  correction  and/or  disciplinary  action  of employees who
choose to violate or neglect safety requirements.

Reional Health & Safet
The  Regional Health  &  Safety  Manager  (RHSM)  is  responsible  for
assisting supervisors  in the selection,  procurement,  issuance,  and
maintenance  of  PPE.     The  RHSM  should  review  each  procurement
request related  to safety,  health or  personal  security,  to assure
that  the  request  is  justified and  appropriate.    The  RHSM  will
determine if  the item, or acceptable  alternative,  is  available in
the Region,  based  on  the Regional inventory of all PPE.   The RHSM
is   responsible    for   identifying  program   ares  that  require
certification.  As a member of  the Region 5 training committee,  the
RHSM  advfites  the  Office  of Personnel  regarding  the  Health  and
Safety Training Requirements of EPA 1440.2, 1440.3,  and OSHA 29 CFP.
1910.120,  and recommends the required  funding.   The regional Health
and  Safety  Manager  shall  be  responsible  for administering  the
Respiratory Protection Program  in Region 5 .

Employees

Employees  are responsible   for the  PPE issued  to them and  are
required  to  wear and use the PPE as prescribed in this  section

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Additionally, the  employee  is responsible for reporting  any damage
and/or malfunction of the PPE issued them.

Employees  assigned PPE  are  required  to s»ign  a  receipt for  such
items.  Information contained in the receipt should  include:

      I.    They   have  received  proper   training  in  the  use  and
           maintenance of these  items.

      2.    They  have  read the safety procedures  and  agree to accept
           the responsibilities  provided therein.

      3.    They  accept responsibility  for the  maintenance and  use
           of  the   assigned   PPE.      Cleaning,   sanitizing   and
           maintaining    a   respiratory  is    included   in   this
           responsibility.      Any  damage,   excessive   wear,   or
           malfunction of equipment must be reported immediately to
           the    individual's   supervisor.       Individuals    are
           responsible  for the  cost of  replacement of PPE which is
           lost,  damaged, 'or stolen through  their own negligence.

      4.    Items provided from  a general supply will be  maintained
           by the  organizational  unit  responsible  for  its  supply
           and issuance.

SELECTION  OF PPE

All  PPE  selected for  use  must  meet  the  standard set  forth  in
Appendix I of. the GLNPO  Health, Safety  and Environmental  Compliance
Manual.       All    PPE,    when   applicable,    must   meet    the
approval/certification requirements set  forth by OSHA regulations.

PPE REQUIREMENTS ON BOARD R/V MDDPUPPY

Standard Level  of  Protection

Personnel  regularly engaged  in  field work must be  equipped with  the
following  minimum  level  of PPE:

      1.    Steel toed shoes/boots

      2.    Safety glasses/goggles  and  face shield when applicable

      3 .    Hard Hat

Field Work of a Hazardous Nature

In addition to  the  above PPE  requirements,  personnel assigned  ~
field activities of  a  hazardous  or  potentially hazardous  nature
such  as  those performed  on  board the R/V Mudpuppy, must be equipp- .
with  the  following PPE:

      1.    disposable protective suit  (tyvek or  saranacs)

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t
     2.   two layers of  disposable gloves (latex and rubber)

     3.   disposable boot  covers

     4.   respiratory protection  as appropriate

     5.   personnel flotation device (type III minimum)

     6.   Exposure  suits   (during  cold  weather  operations  over
          water)

ISSUANCE AND TRAINING IN USE OF PPE

All of the PPE  listed  in  this section,  as  specified  for use  in
field  operations must be issued to the employee  or made  available.
 If PPE  is  required for the  task,  it  must be worn during  sampling
operations.  No one may enter a controlled area  without proper  PPE.
  Personnel issued  PPE  must  be  trained in  the  proper  use   and
limitation  of  such PPE.    All personnel  engaged  in  field  work
activities must receive  training  in compliance with the  provisions
of EPA Order  1440.2 and 1440.3.   Personnel  shall not be permitted
to engage in routine field activities unless they have been  trained
and  certified   to   a   level   commensurate  with  the  degree   of
anticipated hazards.    All personnel  shall  have  a minimum of  24
hours  of  health and safety training prior to  becoming involved  in
routine field activities.   In addition,  employees engaged in field
activities  requiring  the  use  of   respiratory protection  must  be
properly  trained  in the uses  and  limitations of  such devices  and
must be  certified by  a physician  as physically capable of  wearing
such devices.

LEVELS OF PROTECTION

There  are four ba^lq levels of protection:

     Level A - (Provides  the  maximum   protection.     Consists    of
               V     supplied air or SCBA, and a  fully encapsulated
                \,   .protective suit.  Even the breathing apparatus
                 ^-'is inside the suit.

     Level B -       Provides   maximum    respiratory    protection
                     (supplied  air  or   SCBA).     Provides  skin
                     protection equal to  or greater  than level C.

     Level C -       Provides skin protection  requiring  the  use  of
                     personal protective  clothing,  inner  and outer
                     gloves,   boot   covers,   respiratory  protection
                     and general  PPE requirements listed  in Level
                     D.   Generally,  Level C  respiratory protecticr.
                     involves  the  use  of  negative  pressure    :x
                     positive pressure  air purifying respirator.

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     Level D -  Minimum  protection.   Consists   of   common   work
                     clothes,  safety glasses,  hard hat,  steel  toed
                     shoes/boots

Any of  these  levels my be modified, to  a certain extent,  based on
specific hazard assessments.  For example, persons on board the R/V
Mudpuppy generally  work  in a  modified  Level C mode of protection.
Specifically,   all  of  the  skin  and eye  protection requirements of
Level  C protection are  met,  but most of  the  time  respiratory
protection is not necessary.

PPE DONNING AND DOFFING PROCEDURES

Due to  the  potential hazards associated with sediment  sampling on
board the R/V Mudpuppy, standard operating procedures require that,
at a minimum,  modified Level C protection  be worn during  sediment
sampling activities.   However,  levels  of  protection  (ie. use  of
respiratory protection, etc.) may  increase  depending  on  the  degree
of hazard.

When required  to wear  PPE,  you wear  it to  protect  yourself  from
contact  with  potentially  hazardous  materials.   Equally,  proper
donning  and  doffing procedures of  PPE play  and  important  role  in
avoiding contact with potential hazardous materials.   The following
lists  the  proper order  in which Level  C PPE should be donned  on
doffed:

Donning
     1.   Don  PFD  (or  mustang suit  during cold weather)
     2 .   Don  disposable protective suit
     3 .   Don  disposable boots
     4.   Seal boots to  suit  using  duct  tape
     5.   Don   inner   gloves   (latex),   ensure  gloves  are  tucked
           inside of suit  (these  will be the last things  to  come
          off  during doffing  procedure)
     6.   Don  outer gloves (rubber  or  neoprene)
     7 .    Seal outer gloves to  suit with duct tape
     8.   Seal any other necessary  opening with duct  tape
     9.   Don  respiratory protection  if applicable  (if wearing a
          hooded suit, put  hood on after  respirator  is donned;
          mver wear straps of  respirator outside of  hood)
     10.  tifep hard hat,  safety  glasses,  and face shield

Doffing    -
     1.   Wash and rinse hard hat,  face  shield, boot  covers, suit
           and gloves
     2.    Remove tape,  dispose  in proper container
     3.    Remove boot  covers, dispose  in proper container
     4.    Remove outer gloves,  dispose in proper container
     5.    Remove hard  hat,  face shield,  and safety glasses
     6.    Remove disposable suit,  dispose in  proper container
     7 .    Wash and rinse inner  glove
     8.    Remove respiratory  protection,  if applicable
     9.    Remove inner gloves,  dispose in proper container

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Note:   Supplies   may  be   re-used  provided   they   are  properly
decontaminated, properly stored  (ie. in a plastic bag), and are not
damaged.

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USEPA R/V MUDPUPPY
    APPENDIX D

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 1. Long Core Sediment Sampling Using  a  Vibro-Corer  onboard the
                           R/V Mudpuppy

1.1 Overview

The following SOP explains the technique for  collecting  sediment
core samples  (up to 5 m) using a Rossfelder P-4 Vibro-corer.   The
procedures covers the following activities:

 •  Site position.
 •  Securing the vessel for sampling,
 •  Sampling procedure.

Any questions on sampling methods or operation of equipment
should be directed to the following individuals:

Rick Fox
U.S. EPA GLNPO
77 W. Jackson  (G-9J)
Chicago IL. 60604
Phone: 312-353-7979

NOTE: This SOP is written primarily for  illustrating the
technique of sampling and subsampling long sediment core samples
(i.e., up to 5 m).  Details on labeling  and transport are left  to
individual project leads and what is considered appropriate for
the specific project.

1.2 Sample Handling and Preservation

Due to the expense of operating the vessel to collect the sampler
and the analysis costs, every sample is  important.  Any
contamination through mishandling or lack of preservation could
cause a bias in the data estimates.
 • At a minimum, nitrile gloves and Saranac coveralls should be
used during sampling and subsampling activities.
 • Sample containers must be kept free of contamination and
should remain sealed until use.
 • Preservatives should be fresh and dated.
 • Samples should be stored in coolers with freeze packs as socr.
as possible.
 • Cooler* should be checked at a minimum of twice a day to
determin* that the appropriate temperature is maintained.
 • Mode of sample transport must maintain the integrity of the
samples.

1.3 Interferences

A  sampling activity may be collecting more than one type of
sample at a site.  When sampling for benthic organisms will ta>-.-
place, it is  important to collect benchic organism samples pri: •
to  the collection of any other types of  samples,  as other
sampling may disturb the surface benthic community.

-------
All attempts should be made to stabilize the vessel  as much as
possible in order to collect vertical cores.  When the vessel is
moving, the corer can enter the sediments at an angle.

1.4 Safety

In any field operation, emphasis must be place on safety.   Site
operators must be aware of the potential safety hazards  to  which
they are subjected. There is a safety manual specifically
developed for the R/V Mudpuppy.  All personnel on board  must be
familiar with the contents of this document prior to  implementing
any data collection activities, follow all safety protocols and
equipment guidelines, and be prepared for emergency situations.
The ship's captain is the primary authority during vessel
operations.  The captain is also responsible for determining
whether a sampling activity will be implemented during inclement
weather. The sampler is responsible for his/her safety from
potential hazards including but not limited to:

-Electrical.  For obvious problems (fire, scorching,  blown
fuses), turn off the power for the circuit involved and notify
captain (lead).  Never attempt electrical repairs.

-Protection.  The sampler should be prepared for working with
large and or heavy equipment where safety shoes,  head, hand,
protective clothing  (Tyvek or Saranac),  and eye protection are
necessary.  Some sites may require respirators.  Samplers should
have clothing available for weather extremes.

-Sampling.  Never force glassware with unprotected hands.  Care
must be taken around the samplers to avoid injuries and slipping
overboard when positioning samplers.

-Chemicals. Organic solvents and acids are occasionally used for
equipment decontamination and should not be ingested or come int:
contact with bare skin or flame (if flammable).

1.5 Equipment and Supplies

The following equipment and supplies would be needed for a
typical sampling at one station:

vibro-cor«r  (Rossfelder Model P-4)
rolling box  for vibrating head
winch
4" aluminum  core tubes
4" butyrate  core liners
stainless  steel nose cone with core catcher
metal  sampling tray  (15 cm. deep)
HPDE sediment sample bottles
glass  bottles for organic contaminant samples*
ice chests*
Labels*

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markers/pencils*
Plastic bags for sample bottles*
GPS or locational equipment
generator  (230 V, 60 Hz, 3 phase,  14 A)
heavy duty riveter and stainless  steel  rivets
hammer
chisel
nail punch
safety equipment  (i.e., hard hats with  face  shields,  gloves,
Saranac suits, steel toed boots**, boot covers,  safety glasses**)
core caps
hacksaw
carpet knife
flanged pipe
mallet

* must be provided by the grantee
** must be provided by the individual user

1.6 Collection Procedure

This procedure will detail the collection of long sediment core
samples  (up to 5 m) from a site location.  When benthic organism
samples are being collected at the same site, it is important to
collect benthic organism samples  prior  to the collection of
sediment samples to minimize disturbances of the benthic
organisms.

1.6.1 Sample Location

The sample location may be either defined prior to sampling,
where the R/V Mudpuppy would be destined for a particular point,
or the site will be selected during the sampling procedure.
Generally, sites should be located with GPS within approximately
5 meters.  If the vessel is heading to  a pre-determine site, the
locational equipment will be used to locate the site.  However,
actual locational readings should not occur until the vessel is
anchored at the sampling site.  The position is determined by the
captain.

1.6.2 Securing the Vessel

Once on  thm sampling site, the vessel must be secured since
drifting and rotation can cause the coring device to enter
sediments at an angle.

  1) Triple anchor the vessel, as  instructed by the captain.

  2) Establish sampling location with locational equipment  (if
location does not need to be accurately predetermined, this can
be done while sampling).

1.6.3 Sampling Procedure - Vibro-corer

-------
This procedure is used to collect  long  sediment  core  samples.

  1)  Measure the water depth.

  2)  Insert butyrate core  liner into core  tube.   Insert nose
cone into liner as  far as possible.

  3)  Push nose cone and liner  into  core  tube until nose cone  and
core tube rivet holes line  up.  Pop  rivet into place  with
stainless steel rivets.

  4)  Cut of excess butyrate  tubing  (with a hacksaw),  leaving  2
inches of butyrate  tube sticking out of the top  of the core  tube.

  5)  Insert the core tube  into the  vibrating head, making sure
that the butyrate tube slides into the  check valve.

  6)  Tighten the collar to the vibro-corer (three bolts) and
then to the core tube (two  bolts).   If  the  two bolts  are
tightened prior to  the three bolts,  the core tube  may be lost.

  7)  Lift the entire assembly  with  the winch to a vertical
position so that it is suspended just off the bow  of  the sampling
vessel.  Measure water depth.

  8)  Lower the entire assembly until the core nose is  just  above
sediment surface.   Turn on  generator and vibrating head.

  9)  Slowly lower  the vibro-corer by running out  10-20  cm of
cable at a time.  Monitor core  tube  penetration by feeling for
slack in the cable.

  10)  When vibro-corer ceases  to penetrate the  sediment (stops
lowering or is  "refused"),  or the vibrating head is near the
sediment surface, reverse the winch  and pull the unit  from the
sediment.  Do not allow vibrating head  to become imbedded into
the sediments.

  11)  Turn off the power to  the vibrating  head  and the  generat:-.
when the core  "breaks free" of  the sediment and  retrieve coring
unit to dock,  setting the vibrating  head  into the  rolling box.

  12)  Loosen  collar, separate  the core tube from  the  vibrating
head, and decant water from core tube.

  13)  Chisel  off rivets and  remove  the core nose.  Pull the cc:
 liner out of  the core tube.   If the  core  tube does not  pull  our
 it  should be hammered through with a flanged pipe  and mallet.

   14)  Cap  the bottom of the  core  liner with a red cap plug  and
 remove  liner  from the core  tube.   Saw off excess core  liner  at

-------
the sediment surface and cap the top of the tube with a red cap
plug.

  15)  Handle and subsample core as desired,  either on-board or
at a shore-based location.

-------
           5)  Transfer sediment to required sample container.



           6)  Repeat steps 2 through 5 until an adequate volume



of sediment has been collected,  moving the winch arm first, then



the sampling vessel slightly as needed co assure the collection



of surficial sediment.



           7)  Mix the sediments in a stainless steel mixing




bowl.



           8)  Homogenize for 5 minutes.



           9)  Transfer sediment into sample bottles (100 ml to



10 L capacity, as appropriate).   Label each bottle with station



number and other necessary information.



           10)  Place sample bottles into ice chests,  and change



freezer packs as necessary (usually once cr twice a day).

-------
USEPA R/V MUDPUPPY
    APPENDIX E

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    APPENDIX  E: R/V MUDPUPPY  - HAZARD  COMMUNICATION
                             PROGRAM

COMMITMENT

The U.S. EPA  is  firmly  committed to providing  a safe and healthful
work environment  for  all  EPA personnel and  contractors.   In order
to meet this  commitment,  all GLNPO facilities  and Research Vessels
are required  to comply with OSHA's Hazard Communication Standard,
29  CFR 1910.1200.    This  Hazard  communication  program has  been
developed   as   outlined   in   the  GLNPO   Health,    Safety,   and
Environmental Compliance Manual.

RESPONSIBILITIES

Hazard Communication Coordinator

The role  if the Hazard Communication Coordinator on  board  the R/V
Mudpuppy will be fulfilled by the EPA Chief Scientist.

The  responsibility  of  the Hazard Communication  Coordinator  to
ensuring compliance by coordinating the following functions:

     1.    Maintenance of  the hazardous chemical inventory.

     2.    Maintenance  of  a complete  Material  Safety Data  Sheet
           (MSDS)  file and current  employee MSDS notebook.

     3.    Training  of  all  employees  and  contractors  regarding
           chemical safety.

     4.    Updating  of  training  as  new  chemicals   are  added  or
           processes are changed.

     5.    Labeling of all transfer  and waste containers.

     6.    Dissemination    of   hazard   information    to   outside
           contractors.

A successful  Hazard  Communication Program requires  the cooper at irr.
of  EPA  anjfe con tract ing  personnel.   It  is  the duty of  GLNPC
specificail^athe Hazard Communication Coordinator,  to  assure  than
hazard coai^plcation  is given proper priority.   All  personnel  are
encouraged to ask questions  and keep informed  so  that  GLNPO  car.
maintain  a safe  and healthy workplace.

ACCESS  TO TEE WRITTEN PROGRAM

All, or any part, of this  written Hazard Communication Program m-^r-
be available to  all  EPA  personnel,  Contracting  personnel,  and  :
 their  designated representatives.    Interested persons  can  conta:"
 the Hazard Communication  Coordinator for review and copying of tr..
document.

-------
LABELING

In accordance  with the OSHA  Hazard Communication Standard  (29 CFR
1910.1200), all containers  shall  be labeled as  specified below.

     1.    No hazardous chemicals will be accepted  for use on board
           GLNPO  vessels or shipped to any  outside  location unless
           labeled with at  least the following information:

           A.    Identity of the hazardous chemical(s)
           B.    Appropriate hazard warnings for the chemical(s)
           C.    Name  and  address  of  the  chemical  manufacturer,
                importer,  or other responsible party.

     2.    All  spray bottles  or other  transfer containers  will  be
           labeled with at  least the following information:

           A.    Identity of the hazardous chemical(s)
           B.    Appropriate hazard warnings for the chemical(s)

     3 .    The  Hazard Communication  Coordinator is responsible  for
           reviewing labels and assuring that the label  information
           is kept current.

     4.    No  label is  to  be  defaced or removed  when material  is
           received or  in  use.   Any  container  with missing  labels
           will  be  removed from  service  until proper  labels  are
           installed.

MATERIAL SAFETY DATA SHEETS

A Material Safety Data  Sheet  (MSDS)  containing  information require :
by  29  CFR  1910.1200  shall   be   maintained  for  each   hazardc„
substance.  The MSDS  shall be the most current one supplied by  tr-
chemical  manufacturer,  importer,   or  distributor.    MSDS  will  L-
readily available for review by  all  personnel.   Chemicals  brougr.-
on board  the R/V Mudpuppy must  be  accompanied by an  MSDS.   Th.
rule  applies  to  all   EPA  and  contracting  personnel.    Without
MSDS, chemicals will not be permitted  on board.

An  office  file  containing  the  current   chemical  inventory   a:
appropriate*- MSDSs will be  maintained on board the  R/V Mudpuppy
Additionally,  the  R/V Mudpuppy  maintains  a  computer prograirr--
chemical information  system.   The program provides ready  access  -
pertinent  chemical  information.

PERSONNEL  INFORMATION AND TRAINING  POLICY

All EPA personnel and  contractors,  including  temporary contractor
who  may   possibly  be  exposed   to   chemical   hazards  shall
appropriately  informed  and/or  trained concerning  the   potent.
hazards of the chemicals  in  GLNPO facilities.    All  EPA  person:

-------
and contractors, including temporary contractors, shall be  informed
of the details  contained  in  this program,  including an explanation
of the  labeling system,  MSDSs,  and  how to  interpret  and use  this
information.     All  EPA  personnel   and   contractors,   including
temporary contractors,  shall be provided  with additional  training
when  new chemical  hazards  are  introduced or prior  to performing
non-routine   tasks   that   could   involve   exposure  to  hazardous
chemicals.   When  appropriate,  reinforcement  of training  will  be
conducted  through  topics  at  safety  meetings.    The   extent  of
information transmitted to the EPA personnel and contractors during
training sessions will be dictated by  the  degree of chemical hazard
present.  The following source of information will be used during
training sessions:

      1.   The text  of  the  Hazard  Communication Standard  (29 CFR
          1910.1200)

      2.   An  inventory  list  of hazardous chemicals

      3.   Applicable MSDSs

      4.   The written Hazard Communication Program

CONTRACTOR POLICY

Outside   contractors  shall   be  provided   with  all   necessary
information concerning  the potential  hazards  of  the  substances  to
which they may be  exposed  and  appropriate protective  measures
required to  minimize  their  exposure.    Whenever  possible,  the
contractor  or agency management should be provided with  a  list  of
the  hazardous  chemicals  and  the MSDSs   for  the  materials  their
employees may be exposed to by virtue  of their work activities.   In
addition, contractors must have MSDSs on  site for  all potentially-
hazardous chemicals  they use or produce in GLNPO facilities.

-------
USEPA R/V MUDPUPPY
    APPENDIX F

-------
                        USEPA R/V MUDPUPPY
                        CHEMICAL INVENTORY
This sheet must be completed as chemicals  are  brought  on board the
R/V Mudpuppy.  MSDSs must be available  for all chemicals contained
in this  listing.   OSHA requires  that these records be  maintained
for a period of thirty years.

Date      Chemical        Manufacturer        Quantity

-------
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-------
USEPA R/V MUDPUPPY
    APPENDIX G

-------
                                    R/V MUDPIPPY
            SHIPBOARD WINCH DOCUMENTATION 4 \D OPERA TOR STA TLS
NAME OF WINCH:
Manufacturer
Date of mfg
Model
Max Load/Rated Line Puil
Serial No
oounds (

KG)
      Drum Capacity	feet of	inch dia wire rope (	meters of	mm dia rope)

      Drive: Gasoline	 Diesel	 Electric	Electric chain	 Hydraulic	Electro-Hydraulic_
      Horsepower	  Electric power:	VAC.	Hz,	Phase.

      Wire rope payout meter: NO	YES__ Type: Mechanical	 Electrical	
      Drum speed control:    NO	YES__ Type	
      Load sensor:          NO_ YES_ Type	
      Levelwind:            N0_ YES_ Type	

      Location on Ship:	

ANCILLARY EQUIPMENT: (A Frame use, davits, number and size of fairleads, special running
rigging
features)                                   	   	
WINCH OPERATIONAL STATUS AS OF:
TEST LOAD CERTIFICATION: Weight:	pounds. Date certified:	.
WIRE ROPE INSTALLED: Type	 Nominal Dia.	inches.
Length:            feet.
      Maximum safe working load:           pounds. (For sampling, use a safety factor or"
      the wirerope manufacturers listed breaking strength).
      Termination of cable to drum: Type	
       Is cable color coded? NO	YES__ Number of feet from bitter end of rope	color
                                -or-  First rap on drum, color	
                                    Second rap.      color	
                                    Third rap.       color	
QUALIFIED OPERATORS:

	Date:	

	Date:	

                                                       Date:
 RESPONSIBLE SSI AUTHORITY:
 Form UPA SWDOS Rev 7-M4

-------
                     Seaward Services, Inc.
DATE:
                           [WMUDPUPPY
                      CREW  ~ PASSENGER LIST
LOCATION:
Captain:

Engineer:
Marine Technician:
L'SEPA Representative:,
             NAME
                             PASSENGERS
                           ORGANIZATION

-------
USEPA R/V MUDPUPPY
    APPENDIX H

-------
                                                           APPENDIX : H
                                                           VERSION: DRAFT-4/96
                                                           PAGE: 1
R/V MUDPUPPY-CONFINED SPACE ENTRY PROGRAM
PURPOSE

In accordance with the U. S. Environmental Protection Agency Regional Policy, each Division
Office or other unit of the U. S. EPA is responsible for establishing a specific Standard Operating
Procedure (SOP) for each area of potential confined space entry. The SOP must reflect all
activities, specific needs, and exposure potential. Additionally, in accordance with the U. S. EPA
Regional policy, the Great Lakes National Program Office (GLNPO) has implemented a confined
space entry policy (Refer to Appendix E of the GLNPO Health, Safety & Environmental
Compliance Manual). Personnel working on board the R/V Mudpuppy are required to adhere to
both the Regional and Division policies. This program has been developed in accordance with
OSHA 29 CFR 1910.146 and U. S. EPA policies as outline in EPA Order 1450.4.  All personnel
working on-board the R/V Mudpuppy are required to comply with the policies and procedures set
forth in this document.
CONFINED SPACE DEFINED

A confined space is a space which has any one of the following characteristics:

       •      limited openings for entry and exit

       •      unfavorable natural ventilation

       •      not designated for continuous worker occupancy

       •      contains a hazardous atmosphere or has the potential for such

A confined space may have a combination of these characteristics, which can complicate work
and rescue opentions in and around these spaces. A survey of the R/V Mudpuppy has identified
the forward and rfer holds of the vessel as confined spaces.


HAZARDOUS ENVIRONMENTS OR ATMOSPHERES

As defined, a hazardous atmosphere is one which presents a potential for death, disablement,
injury, or acute illness. The atmosphere in  a confined space may be extremely hazardous beca».
of the lack of natural air movement. This charactensuc of confined spaces can result in oxygen -
deficient atmospheres, flammable atmospheres and/or toxic atmospheres.

-------
                                                              APPENDIX :  H
                                                              VERSION: DRAFT-4/96
                                                              PAGE.-2
Oxv gen-Deficient Atmospheres:
An oxygen-deficient atmosphere has less than 19.5% available oxygen. Any atmosphere with less
than  19.5% oxygen should not be entered without an approved Self-Ccntained Breathing
Apparatus (SCBA).  The oxygen level in a confined space can decrease because of work being
done in the area (e.g. welding, cutting, or brazing), it can be decreased by certain chemical
reactions (e.g. rusting), through bacterial action (e.g. fermentation), or it can be decreased if
oxygen is displaced by other gases, such as carbon dioxide or nitrogen. Total displacement of
oxygen by other gas will result in unconsciousness, followed by death.

Rammahle Atmospheres:

Two elements make up a flammable atmosphere: I) the oxygen content in the air; and 2) a
flammable gas, vapor, or dust in the proper mixture. Different gases have different flammable
ranges.  If a source of ignition (e.g. a sparking tool) is introduced into a space containing a
flammable atmosphere, an explosion will result.

.An oxygen-enriched atmosphere (above 23.5%)  will cause flammable materials, such as clothing
and hair, to burn violendy when ignited. Therefore, when ventilating  a confined space never use
pure oxygen.  Ventilate with natural air.

Toxic Atmospheres:

Most substances (liquids, vapors, gases, mists, solid materials, and dusts) should be considered
hazardous in a confined space. Toxic substances can come from: products stored in the area;
work being performed in the area; areas adjacent to the confine space; or equipment in the area
(e.g. combustion engine). Toxicants produced in and around the area can accumulate in the
confined space creating a hazardous environment.
RFQI'TRFMENTS

Testing The Atfjyisphffi

Pnor to entering a confined space, atmospheric testing must be conducted to determine if the
atmosphere is safe  for entry.  If testing reveals oxygen-deficiency, or the presence of toxic gases
or vapors, the space must be ventilated and re-tested before entry. If ventilation is not possible
and entry is necessary (e.g. for emergency rescue), entry personnel must don supplied air
respiratory protection.

-------
                                                           APPENDIX : H
                                                           VERSION: DRAFT-4/96
                                                           PAGE: 3

NEVER TRUST YOUR SENSES TO DETERMINE IF THE AIR IN A CONFINED SPACE IS
SAFE! YOU CAN NOT SEE OR SMELL MANY TOXIC GASES AND VAPORS, NOR CAN
YOU DETERMINE THE LEVEL OF OXYGEN PRESENT.

Ventilation

Ventilation by a blower or fan may be necessary to remove harmful gases and vapors from a
confined space.  The method and equipment chosen are dependent upon the size of the confined
space opening, the gases to be exhausted (e.g. are they flammable ?), and the source of makeup
air.

Under certain conditions where flammable gases or vapors have displaced the oxygen level, but
are too nch to burn, forced air ventilation may dilute them until they are within the explosive
range.  Also, if inert gases (e.g. carbon dioxide, nitrogen, argon) are used in the confined space,
the space should be well ventilated and re-tested before a worker may enter.

Ventilation should be continuous where possible, because  m many confined spaces the hazardous
atmosphere will form again when the flow of air is stopped.

Isolation

Isolation of a confined space is a process where the space is removed from service by:

       •      Lockout/tagout - Lockout all electrical sources, preferably at disconnect switches
             remote from equipment

       •      Blanking and  Bleeding - pneumatic and hydraulic lines

       •      Disconnecting - belt and chain drives, and mechanical linkages on shaft-driven
             equipraent.where possible, and

       •      SiCoring - mechanical moving parts within a confined space with latches, chains.
             cfecks, blocks, or other devices.

Respirators

Respirators are devices that can allow workers to  safely breathe without inhaling toxic gases or
particles.  Two basic types are air-purifying, which filter dangerous substances from the air: and
air-supplying, which deliver a supply of safe breathing air from a tank or an uncontaminated area
nearby.

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                                                             APPENDIX : H
                                                             VERSION: DRAFT-4/96
                                                             PAGE:4

Only air-supplying respirators should be used in confined spaces where there is not enough
oxygen !

In selecting the proper respirator for the job, knowledge of the hazard, and thorough training in
the use and limitations of respirators are very important. The issuance and use of respiratory
equipment must be performed in accordance with the policies set forth in the GLNPO Health,
Safety and Environmental Compliance Manual. Questions regarding the proper selection and use
of respirators on board the R/V Mudpuppy should be addressed by the Regional Safety Manager
or his/her designee.

Personal Protective Equipment

In addition to respiratory protection, the" proper selection of Personal Protective Equipment (PPE)
is very important.  Selection must be based on the potential health and safety hazards associated
with work activities.  Prior to work activities an  assessment must be conducted to evaluate the
need and determine proper selection of PPE. Minimum protective equipment requirements should
include: hard hat, steel toed shoes/boots, safety glasses, and harness.

Standby Rescue

A Standby person must be assigned to remain  outside of the confined space and be in constant
contact (visual or speech) with the person inside. The standby person should not have any other
duties but to serve as standby and know who should be notified in the event of an emergency.
Standby personnel should not enter a confined space until help arrives, and then only with proper
protective equipment, life lines, and respirators.

Over 50% of workers who die in confined spaces are would-be rescuers.  Rescuers must be
trained in and follow established emergency procedures and use appropriate equipment and
techniques.  Steps for safe rescue must be included in all confined space entry procedures.
Rescue should be well planned and drills should he conducted on  emergency procedures.
Unplanned rescue, such as when someone instmctne!> rushes in to help a downed co-worker. o.~
easily result in a double fatality.
 TRAINING

 In accordance with OSHA 29 CFR 1910.146 and EPA Policy, prior to participating in confined
 space entry, personnel must be properly trained in the uses of respiratory protective equipment.
 monitoring equipment, personal protective equipment, and be knowledgeable in the confine spav
 programs implemented by the GLNPO. Team leaders must be trained in first aid and CPR and -
 knowledgeable in the hazards associated with confined spaces.

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                                                         APPENDIX : H
                                                         VERSION: DRAFT-4/96
                                                         PAGE:5
PRF-ENTRY CHECKLIST/CONFINED SPACE ENTRY PERMIT

A pre-entry checklist/confined space entry permit must be completed prior to entering a confined
space. This checklist/permit can be found as Attachment 1 to this document. Pemiits must
remain on file on board the vessel. Additionally, copies of the permits should be forwarded to the
GLNPO Safety Representative on a quarterly basis.

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                                                     APPENDIX :  H
                                                     VERSION: DRAFT-4/96
                                                     PAGE:6
ATTACHMENT 1
R/V MTJDPUPPY - PRE-ENTRY CHECKLIST/CONFINED SPACE ENTRY PFRMTT

The following checklist must be completed and the permit signed by a competent person/team
leader prior to entering a confined space.

DO NOT ENTER A CONFINED SPACE UNTIL YOU HAVE CONSIDERED EVERY
QUESTION, AND HAVE DETERMINED THE SPACE TO BE SAFE.

SECTION A:

YES/NO

	      Is entry necessary? Specify purpose:	
SECTION B:

            TESTING

	      Are the instruments used in atmospheric monitoring properly calibrated?

	      Was the atmosphere in the confined space tested9

	      Was the oxygen level at least 19.5% - not more than 23.5%?

	      Were toxic, flammable, or oxygen-displacing gases/vapors present?
            -hydrogen sulfide
            -carbon monoxide
            -methane
            -other (list)	


SECTION C:

            MONITORING

	      Will the atmosphere in the space be monitored while work is going on?

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                                                            APPENDIX :  H
                                                            VERSION: DRAFT-4/96
                                                            PAGE:?
             Continuously?

             Periodically? (If yes, give intervals:
SECTION D:

             VENTILATION

             Has the space been ventilated for at least 15 minutes before entry?

             Will ventilation be continued during entry9

             Is the air intake for the ventilation system located in an area that is free of
             combustible dusts and vapors and toxic substance?

             If atmosphere was found unacceptable and then ventilated, was it re-tested before
             entry?

If reason for entry does not include to use of chemicals, welding equipment, or involve any
renovation activities or additional potential hazards associated with work activities, you may skip
sections E through G and proceed to Section H.


SECTION E:

             ISOLATION

	      Has the space been isolated from other systems?

	      Has electrical equipment been locked out11

	      Have disconnects been used where possible?

	      Has mechanical equipment been blocked, chocked,  and cmengaged where
             necessary?

	      Have lines under pressure been blanked and bled0
SECTION F:

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                                                           APPENDIX : H
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                                                           PAGE: 8
             CLOTHING & EQUIPMENT
             Is special clothing required (boots, protective clothing, safety glasses, hard hat)?
             (If so, specify:	)

             Is special equipment required (rescue equipment, communication equipment, etc.)?
             (If so, specify:	)

             Are special tools required (non-sparking)?
             If so, specify:	)
SECTION G:
             RESPIRATORY PROTECTION
             Are MSHA/NIOSH approved respirators of the type required available on site?

             Is respiratory protection required (air-purifying, supplied air, SCBA, etc.)
             (If so, specify type:	)

             Can you get through the opening with a respirator on? (If you don't know, find
             out before you try to enter).
SECTION H:
personnel
TRAINING

If respiratory protection is required (e.g. during renovation activities) have
       been trained in the proper use of a respirator?

Have personnel received first aid/CPR training?

Have personnel been trained in confined space entry and do know what hazards
to look for?
SECTION I:

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                                                            APPENDIX : H
                                                            \ Br*SION: DRAFT-4/96
                                                            PAGE:9

             Will there be a standby person on the outside in constant visual or auditory
                    communication with the person on the inside?  If so. name
             Will the standby person be able to see and/or hear the person inside at all times?

             Has the standby person been trained in rescue procedures?

             Will safety lines and harness be required to remove a person?

             Rescue procedures available to be followed in the event of an
                    emergency?

             Are you familiar with emergency rescue procedures?

             Do you know who to notify  and how in the event of an emergency?
SECTION J:

             EERMU

Emergency Numbers:	

Authorized Entrant(s):	
Duration of Entry:	to	. Note: Duration of entry is not to exceed 12
hours. If conditions exist which may change or have an effect on the health and safety status of
the confined space the permit must be re-issued.

Signature of this permit is an authorization in writing that the space has been tested by a qualified
person, and thai the space is safe for entry; following the pre-cautions listed.

Entry is permitted: (Signature)	    Date:	

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USEPA R/V MUDPUPPY
    APPENDIX I

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                                                        APPENDIX: I
                                                        VERSION: FINAL/MAY 1997
                                                        PAGE: I of 10

R/V MUDPUPPY - SITE SAFETY PLAN (insert project name)

This page must be completed, signed and dated prior to engaging in field activities.
Project Site Location:

EPA Project Manager:

EPA Site Safety Officer:

EPA Plan Preparer:

EPA Plan Reviewer:

Preparation Date:

Expiration Date:
APPROVED BY:          U.S. EPA Region V Health & Safety Manager
                         (Signature and Date) - IF NECESSARY


                         GLNPO Health and Safety Management Advisor


                         (Signature and Date) - IF NECESSARY


                         GLNPO Health & Safety Representative/Consultant


                         (Signature and Date)


                         GLNPO Project Manager


                         (Signature and Date)

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                                                            APPENDIX: I
                                                            VERSION: FINAL/MAY 1997
                                                            PAGE: 2 of 10
LO    PURPOSE

In accordance with OSHA 29 CFR 1910.120 and EPA policy, a Site Specific Safety Plan must
be completed prior to engaging in sampling activities of sites were hazardous constituents are
present or when the potential for hazardous constituents exist.  The plan is designed to identify,
evaluate, and control safety and health hazards and provide for emergency response.  To ensure
that proper health and safety measures are implemented during sampling activities on board the
R/V Mudpuppy, all onsite personnel are required to adhere to the contents of this document as
well as all applicable health and safety requirements implemented by the Great Lakes National
Program Office (GLNPO).

2J)    APPLICABILITY

The provisions of the plan are mandatory for all on-site personnel  This plan has been developed
under U. S. EPA guidelines and complies with  applicable regulations, including Occupational
Safety and Health Administration (OSHA) standards [29 Code of Federal Regulations (CFR) 1910
and 1926].

U. S.  EPA-GLNPO will require that all contractors and subcontractors follow the health and
safety  requirements listed below:

       •     Employees must have appropriate training [40-hour OSHA required (29 CFR
              1910.120) health and  safety course  for hazardous  waste  workers, or certified
             equivalent training and 8-hour update training].

       •     Personnel working at hazardous waste sites must have had an annual physical (or
             physician's waiver for biennial physical) and be certified "fit for duty" and "fit for
             respirator use", if necessary, by a qualified  physician.

       •     Proof of training and physical must be obtained before  site work may  begin.

       •     Personnel must have appropriate personal protective equipment for the specific job
             (i.e. hard hat, safety shoes, respirator, hearing protection,  gloves, etc.)

       •     All equipment and field operations must meet applicable safety standards and
              satisfy  an inspection by GLNPO Project Manager or his/her  designee.  Unsafe
              equipment or operations will necessitate shut down of the job.

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                                                           APPENDIX: I
                                                           VERSION: FINAL/MAY 1997
                                                           PAGE: 3 of 10
3.0    SITE CHARACTERIZATION AND ANALYSIS
Prior to field activities, this document must be completed by the GLNPO Project Officer and
submitted to the appropriate Health and Safety Representatives (i.e., GLNPO Health & Safety
Consultant for moderate to low hazard sites, GLNPO Section Team Leader, and GLNPO Safety
Team Leader).  Based on the  site characterization and analysis, it shall be determined to what
extent this plan will be detailed. Sites  with the potential to pose acute hazards (high hazard
ranking)  shall be submitted to the  GLNPO Health and  Safety Management Advisor  and the
Regional Health & Safety Manager for approval within twenty days (or as soon as possible) prior
to sampling  activities.  Completed plans  of high ranking and moderate  hazards  must be
signed/approved  by appropriate personnel prior to field activities.

General Information

Site Location:

Objective;

Material Types:     Liquid:	Solid:	Sludge:	Gas:	Sediment:	
Characteristics:     Corrosive:	Ignitable:	Radioactive:	Volatile:	
                    Toxic:	Reactive:	Unknown:	

Unusual Site Features:

Status:

      Background Review: Completed:	    Preliminary:	  Incomplete:

      Documentation/Summary (Overall Hazard):       Serious/High:	
             Moderate:	      Low:	    Unknown:	

Brief Summary of Hazard Evaluation:

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                                                          APPENDIX: I
                                                          VERSION: FINAL/MAY 1997
                                                          PAGE: 4 of 10
4.0    SITE DESCRIPTION
Date of Activities:
Location of Sampling Site(s):
Known or Potential Hazards:
Additional Information:
5.0    OBJECTIVE: (describe actions and tasks to be accomplished; i.e. identify contaminated
       sediments, sampling methods)
6.0    ONSITE ORGANIZATION & COORDINATION

Project Team Leader:	

Site Safety Officer:	

Field Team Leader:	

Field Team Members:                            	
Federal Agency Reps:

State Agency Reps:

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                                                           APPENDIX: I
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE: 5 of 10
Local Agency Reps:

Contractors:
All persons onsite must be listed above.  All activities on site must be cleared with the Team
Leader.
7J)    ONSITE CONTROL

	(Name) has been designated as onsite control coordinator.

The prevailing wind conditions are	.  Whenever possible, sampling activities should occur
downwind  of the cabin area.  When sampling areas where the potential exist for airborne
concentrations of contaminants could exceed  OSHA Permissible  Exposure Limits  (PELs),
sampling activities must occur downwind of the cabin area.


8J)    HAZARD EVALUATION

The following substance(s) are  known or suspected to be on site:

	PCBs    	  Metals (Please  Circle - Including:  Mercury, Arsenic,  Cadmium,  Lead,
                                                Chromium, Zinc, and Nickel)
    PAHs        APIs
Additional hazards not listed above:
The primary hazards associated with substance are as follows: [for hazard information on PCBs,
PAHs, AHs, and Metals (including: Mercury, Arsenic, Cadmium, Lead, Chromium, Zinc, and
Nickel) listed above, refer to attachment 1 of this document]

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                                                           APPENDIX: I
                                                           VERSION: FINAL/MAY 1997
                                                           PAGE: 6 of 10
In addition to the substances listed above, the following analytical chemicals are expected to be
brought on site (MSDSs must be accompany any chemicals brought on board):
Hazardous substance information form(s) for the involved substance(s) have been completed and
are attached.
9J)    PERSONAL PROTECTIVE EQUIPMENT

Based on the evaluation of potential hazards, the following levels of personal protection have
been designated for use for the applicable wok tasks:

NOTE - Under normal operations, the minimum Personal Protective Equipment (PPE) required
to be worn by personnel working on deck aboard the R/V Mudpuppy include; hard hat, steel toed
footwear, tyvek coveralls, boot covers, personal floatation device, and double gloves (e. g. Level
D - modified level D/C; no respiratory protection).  Modification to the level of protection should
be specified in the space provided below.

Location                         Job Function               Level of Protection

On deck during sampling activities	A     B     C     D

Above cabin during sampling	A     B     C     D

Sample Prep	A     B     C     D

Inside cabin area	A     B     C     D

After deck	A     B     C     D

Specific protective equipment for each level of protection (specify; i.e.  SCBA, Tyvex, etc.):

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                                                           APPENDIX: I
                                                           VERSION:  FINAL/MAY 1997
                                                           PAGE: 7 of 10
10.0   ONSITE WORK PLAN
(Include all tasks to be performed, persons or agency performing tasks, and necessary precautions
to be implemented while performing tasks)

Under usual circumstances the following tasks are performed on board the R/V Mudpuppy:

•      Sediment collection using the vibracore - conducted by the Mudpuppy Field Crew

•      Sediment extraction from the vibracore - conducted by the Mudpuppy Field Crew

•      Packaging of sediment samples for transportation - conducted by the Mudpuppy Field
       Crew

•      Boat positioning and anchoring - conducted by the Mudpuppy Field Crew

The following additional activities will be conducted on board (specify tasks  and  personnel
performing tasks):
The work party was briefed on the contents of this plan at (Time & Date)
11.0   COMMUNICATION PROCEDURES

Channel	has been designated as the radio frequency for emergency communication.

	(# of horn  blast) is the emergency signal to indicate that all personnel should
leave the sampling area.

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                                                           APPENDIX: I
                                                           VERSION: FINAL/MAY 1997
                                                           PAGE: 8 of 10
The following hand signals should be used in the absence of verbal communication:

       Hand gripping throat = out of air, can't breath.
       Hands on top of head = need assistance
       Thumbs up = OK, I'm all right, I understand
       Thumbs down = No, Negative
12.0   DECONTAMINATION PROCEDURES

Personnel  and equipment leaving the  Sampling area  (Exclusion Zone)  shall be thoroughly
decontaminated.  The  standard  decontamination protocol on board the R/V Mudpuppy  is as
follows:

•      Decontamination is conducted in Level D PPE

•      Water is used as a decontamination solution using a garden hose

•      Proper doffing  procedures shall be implemented following decontamination

If other decontamination procedures are required,  please list equipment required and procedure(s)
implemented:
13.0   SITE SAFETY & HEALTH PLAN

	(Name)) is the designated Site Safety Officer and is directly responsible for
the safety recommendations on site.

Emergency Medical

	&	(Names) are qualified and designated as first aid
responders on site.

	(name) Medical facility is located at	(address).

Local ambulance service is available  from	at 	(phone).  Their
response time is	.

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                                                             APPENDIX: I
                                                             VERSION: FINAL/MAY 1997
                                                             PAGE: 9 of 10
First aid/safety equipment is available as follows:

       Emergency eye wash is located on the bow of the vessel
       First aid kit is located inside the cabin area
       PFD's are located inside cabin area
       Ring buoys are located forward and aft
       Fire extinguishers throughout vessel
Emergency medical information for substances present or suspected to be present:

Substance            Exposure symptoms         First Aid Instructions
Environmental Monitoring

The following environmental monitoring equipment shall be used on site at specified intervals
(cross out if not applicable):

Combustible Gas Indicator - continuously/hourly/daily/other	
O2 Monitor -  continuous/hourly/daily/other	
Colorimetric tubes (type)	continuously/hourly/daily/other	
HNU/OVA - continuously/hourly/daily/other	
Geiger Counter - continuously/hourly/daily/other	
Other	(specify)- continuously/hourly/daily/other
Emergency Procedures

The Site  Safety Officer shall be notified of any on  site emergencies and be responsible for
ensuring that appropriate procedures are followed.

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                                                            APPENDIX: I
                                                            VERSION: FINAL/MAY 1997
                                                            PAGE: 10 of 10
PPE Failure
If a site worker experiences personal protective equipment failure they shall immediately leave
the area and not re-enter until equipment has been repaired or replaced.

Other Equipment Failure

If equipment on site fails to operate properly, the Team Leader and Site Safety Officer shall be
notified and then determine the effect of this failure on continuing operations.

Personal Monitoring

The following personal monitoring will be in effect on site:

Personal exposure sampling - (describe sampling program, include equipment types)	
Medical Monitoring

The  expected air temperature will be 	degrees F.  If it is determined that heat stress
monitoring is  required  (mandatory if over  70 degrees F) physiologic monitoring must be
conducted in accordance with the Heat Stress Monitoring Program found in Appendix	of
the R/V Mudpuppy Supplemental  Health, Safety and  Environmental Compliance Manual.
Monitoring procedures include monitoring body temp, pulse, and fluid intake.
14.0   SITE SPECIFIC ORIENTATION

This is to  certify that all site personnel have read the above plan  and are  familiar with its
provisions.

Site Safety Officer (name)	(signature)

Team Leader (name)	(signature)

Other Site  Personnel:
       (name)	(signature)

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                                                           APPENDIX: I
                                                           VERSION: FINAL/MAY 1997
                                                           PAGE: 1  of 2
ATTACHMENT 1:  HAZARD EVALUATION
The following substance(s) are known or suspected to be on site: (Circle if applicable)

 Arsenic, Cadmium, Chromium, Lead, Mercury, Zinc, PCBs, PAHs, oil and grease.

The primary hazards associated with substance are as follows:

Arsenic - oxidizes rapidly when exposed to atmosphere.  Oxidation products of arsenic are more
toxic. Oxidation product Arsine -  extreme acute  toxicity can  occur within  a few hours at
concentrations of 3-10 ppm.   Other systemic effects  include:  poisonous by inhalation and
ingestion, human carcinogen, irritation of digestive tract, decrease in production of red and white
blood cells,  abnormal heart function, blood vessel damage,  liver and/or kidney damage, and
impaired nerve function.  There  are also latent symptoms associated with exposure to  arsine.
These symptoms include: headache, dizziness, garlic odor of breath, numbness, chills and tingling
of hands and feet,  nausea, vomiting,  abdominal cramping, abdominal tenderness and rigidity,
bronzing of the skin, pulmonary edema, jaundice.  The OSHA PEL/TWA for Arsine is O.OSppm
(0.2 mg/m3).

Cadmium - Human carcinogen, inhalation hazard; pulmonary edema, ingestion hazard; coughing
tightness of chest,  headache,  chills, muscular ache,  anosmia, emphysema, nausea,  vomiting,
diarrhea, mild edema.  The OSHA  PEL/TWA for  Cadmium is  0.2  mg/m3  with a ceiling
concentration limit of 0.6 mg/m3  for dusts and 0.1  mg/m3 with a ceiling concentration limit of
0.3 mg/m3 for fumes.

Chromium - Potential human carcinogen, effects lungs,  respiratory  system, and skin, irritant to
mucous membranes, can cause ulcerations of nares and respiratory tract.  The OSHA PEL/TWA
for Chromium is 1  mg/m3.

Lead - Inhalation, contact and ingestion hazard; weakness, insomnia, abdominal pain anemia,
tumors hypotension, etc. Target organs include the GI tract, Central nervous system, kidneys, and
tissue.  The OSHA PEL/TWA is 0.100 mg/m3 (air concentrations must be maintained at a level
by which blood lead level remain O.080 mg/lOOg  of whole blood  [OSHA  1910.1025]).

Mercury - Inhalation, absorption, ingestion and contact hazard; causing dizziness, blurred vision,
emotional disturbance, diarrhea,  nausea, vomiting,  skin burns, etc.   Target organs include  the
Central nervous system, kidneys, eyes, and skin. The OSHA PEL/TWA for Mercury compounds
is  0.01 mg/m3.

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                                                           APPENDIX: 1
                                                           VERSION: FINAL/MAY 1997
                                                           PAGE: 2 of 2
Zinc - Can effect the respiratory system, skin, and eyes.  Symptoms of exposure include: dry
mouth, coughing, conjunctivitis, chills, tightness of chest, headache, cramps, blurred vision, low
back pain, vomiting, etc. OSHA PEL/TWA varies dependent upon the specific zinc compound.
The PEL for Zinc oxide fumes is 5 mg/m3.

PCBs - Inhalation, absorption, contact, and ingestion hazard. Irritant to the eyes and skin; dermal
carcinogen.  Target organs - skin, eyes, and liver. The OSHA PEL/TWA for PCBs is 0.5 mg/m3
for skin.  NIOSH has established an REL  of 0.001 mg/m3.

PAHs - (Polynuclear Aromatic Hydrocarbons) Inhalation,  absorption, ingestion, and contact
hazard.  Target organs  - blood, CNS, skin, bone marrow, eyes, kidney, liver, and respiratory
system.  Signs and symptoms of exposure include but are not limited to irritation to the mucous
membrane, head ache,  dizziness, staggering, fatigue, depression, dermatitis, etc.  The OSHA
PEL/TWA for PAHs varies with the chemical composition; check  appropriate references for
specific PELs.

Nickel - NIOSH has identified Nickel as an occupational human carcinogen.  Hazards: Inhalation,
ingestion, and contact hazard. Target organs include lungs, paranasal sinus, and CNS. Signs and
symptoms of exposure  include: head  ache, vertigo, nausea, vomiting, epigastric  pain, cough,
weakness, pneuitis, delirium, convulsions.  The OSHA PEL/TWA for Nickel is o.l mg/m3 for
soluble compounds and 1 mg/m3 for metals and insoluble compounds.

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                                                     SECTION: APPENDIX O
                                                     VERSION:  FINAL/MAY 1997
                                                     PAGE: COVER
APPENDIX O: GLNPO POLICY, MEMORANDUMS,  STANDARD OPERATING  PROCEDURES,
            REGULATORY UPDATES:

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         USEPA GLNPO
STANDARD OPERATING PROCEDURES
             FOR
      WINTER OPERATIONS
        December 1994

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                           DISCLAIMER

   Approval of this document by the Great Lakes Program Office
  (GLNPO) does not  signify that  the  contents represent the views
and policies of the USEPA,  nor does the mention of trade names or
  commercial products constitute endorsement or recommended use.

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INTRODUCTION

This document describes the Standard Operating Procedures  (SOP), as
required by the USEPA Great  Lake  National  Program Office (GLNPO)
Maritime Safety Program,  for  winter sampling  activities.  This SOP
has been developed  with  the  objective of  minimizing  the hazards
associated with winter sampling operations.

This SOP applies to  all EPA personnel, EPA  contractors or federal,
state, or local government agencies, and persons who operate or are
passengers  on  board USEPA  GLNPO vessels  during  winter  work
activities.

LIFESAVING AND SAFETY EQUIPMENT

     Rescue   Boats     At   all  times  during  winter  sampling
     activities, a rescue boat  must be outrigged into a deployable
     positj.on.  Members  of  the crew responsible for man overboard
     rescue  procedures   must  be  well  trained  and  readied  for
     response activities. A response time of  three minutes must be
     demonstrated by response team personnel.

     All  lifesaving equipment,  such  as  lifelines,  ring  buoys,
     rescue  boats  and   associated equipment,  must  remain  un-
     obstructed at all times.

PERSONAL PROTECTIVE EQUIPMENT

     Harnesses/Lifelines  -  When working on deck,  specifically on
     the fantail  or  around  the perimeter  of  the vessel,  persons
     must wear safety harnesses and a lifeline; should be rigged and
     used.

     Personal  Flotation  Devices  (PFDs)  -  All  persons  working on
     deck must wear, at  a minimum,  a type III PFD.

     Hard Hats -  Persons working on the bow  of the vessel,  or in
     any area  where  over head  hazards may be  present, must  wear
     hard hats.   (Refer to  Protective  Clothing, Head  Covering
     section for additional  information)

     Protective   Clothing      Humans   are   thermally   sensitive
     creatures, and  as   such,  must pay  close  attention to  the
     temperatures in which they vork.   The most  common  types  of
     cold injuries include hypothermia, a dangerous lowering of the
     body's temperature,  and frostbite. To  prevent hypothermia and
     frostbite, persons must  be aware of the body  as a heat source
     and the  importance  of clothing and insulation.   A  critical
     element  in  regulating heat  loss is  the type of  fabric  one
     chooses to wear.  The insulating and ventilating properties of
     a  given   fabric  or  fill  will determine  how well  one  can
     moderate  body  temperature under  certain  conditions.    The

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following is a list of recommended personal clothing for use
by persons involved in winter sampling activities:

1.   Outer Shell/Mustang Suit™ - Outer layer of clothing which
     is capable of affording protection from wind, rain, and
     cold.   Should be large enough  to  fit comfortably over
     insulating  layers  of  clothing.   A  Mustang Suit,  in
     addition  to  providing a  warm  outer shell,  provides
     flotation  and insulation  from  cold  water  immersion.
     Mustang  apparel  provides a  barrier  to  the  cold  water
     outside  the  garment and allows  your body to heat the
     water  that's  inside,   thereby   increasing  cold  water
     survival time.

2.   Boots - Must be of  the steel toe type for those working
     on deck.   Should be insulated or  capable of affording
     room for insulated socks.  Should be waterproof and slip
     resistant.

3.   Head Covering - Probably one  of the most important items
     in protective clothing for cold work environments.  With
     the  brain receiving  20 percent of  the body's  blood
     supply, and 50 percent of our body heat is lost through
     our head, the conservation of heat  in the head will mean
     that our bodies will have more heat available to heat our
     hands and  feet.   Wool is the recommended fabric  since
     wool   fibers   provide  insulation   while   remaining
     "breathable"  and  are  capable of wicking  moisture  away
     from the  body.   Thus  making it possible for wool  to
     provide warm insulation even when wet.  Keep in mind that
     the hat must be capable of being worn under a hard hat.
     There are hats available which are specifically designed
     to be worn with hard hats.

4.   Gloves  -  Warm water resistant  gloves or those  gloves
     capable  of  providing  warmth when wet  (polypropylene,
     wool) should be used to protect the hands from exposure.
     If reguired to perform fine  work with fingers,  one may
     want to consider using a two layer system when choosing
     gloves.  This  will  provide optimum warmth when  needed,
     with the ability  to remove  the  outer layer when it  is
     necessary  to  perform  fine  work.  If  liners are  used
     (inner  glove)  they should be  of  a  fabric capable  of
     wicking moisture away  from the  skin.   Polypropylene  or
     wool are examples of this type of fabric.

5.   Thermal Underwear -  Clothing  designed to be worn  against
     the  skin  is  a crucial part  of   cold  weather  clothing.
     Cotton  fabrics  are not  recommended  for  cold and wet
     environments because of its'  loss of  warming  properties
     when wet and its inability to  wick moisture away from the
     skin.   Thermal  underwear constructed  of  polypropylene

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          fibers  provide good  insulating  values  in  a maritime
          environment.   Polypropylene  reduces  heat  stress  and
          increases comfort  and warmth by  wicking moisture away
          from the body.

     6.   Socks  -  Warm socks made  of polypropylene  or wool are
          recommended.  Make sure to have  sufficient  supply  in the
          event they become wet.

OPERATION LIMITATIONS

     Icing -  The  effects of icing on a vessel  is  a reduction in
     stability due to  an increase in displacement and a rise in
     vertical center of gravity.  In addition, a heeling movement
     may also develop  due to an  off-center  accumulation of ice.
     Ship  operators  must  be  aware  of  the dependency of  ice
     accumulation on the  vessel heading.   The need  for the ship
     operator to make  good a  specific course can greatly influence
     the symmetry or asymmetry of ice accumulation.

     When a  vessel operates in areas where  icing  conditions are
     present, the operator must  be aware that  icing increases with
     the amount of time  spent in  icing  conditions.   Attachment 1
     contains  basic  graphs  which  can  be  used to  provide  the
     operator with a  means  of  estimating the likelihood of  ice
     accumulation on  the vessel.   Generally,  any time the  air
     temperature  is  below  28 F,  icing  can  occur.   Wind  speed,
     vessel heading and air temperature must  all be considered when
     calculating expected icing conditions.

     Safe operations  as  well as  personal safety can  be greatly
     increased by  establishing  operational  limitations  based  on
     weather conditions.  Staying abreast of  current conditions and
     developing conditions is the responsibility of the Shipmaster.
     Maximum  operating  conditions  are  discretionary  and  final
     determinations should be made with  consultation of the  USEPA
     Chief Scientist and the Shipmaster,  with the Shipmaster having
     final authority.

DECK EQUIPMENT

     During icing conditions, all  deck equipment should be covered
     and secured.  Derricks,  booms and like items should be stowed
     in the lowered position.

HEALTH AND SAFETY

     Restricted Areas  -  There are  a multitude of hazards which can
     occur in heavy weather.   Persons involved in winter work who
     are exposed to weather must be aware of the potential hazards
     and safety requirements associated  with work activities.   To
     minimize the potential  hazards associated  with winter  work

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activities,  several areas  on  board  the  vessel  have been
designated as restricted  areas.   Weather decks subjected to
seas are off  limits unless  permission has been granted from
the  Captain,  and when  applicable,  in conjunction with the
Health and Safety Officer.   Such  areas  include, but are not
limited to, the following:

1.   Top of Pilot House

2.   Mast

3.   Rosette Work Platform

4.   The 02 Deck

5.   01 Deck

6.   Starboard & Port Weather Decks

7.   Stern

Movement  throughout the  vessel  should  be  limited to  the
interior whenever possible.

Buddy  System -  When involved  in activities  on  deck,  all
persons will comply with the buddy system.  The buddy should
be capable of providing his  or  her  partner with assistance,
observe his or her partner for signs  and symptoms of exposure,
periodically  check   the  integrity of  his  or   her  partners
protective clothing, notify  the  bridge or others in the event
of an emergency or need of assistance.  The Buddy System alone
may not be sufficient to ensure  that help will be provided in
an emergency.  Therefore, persons on  deck must be in  line-
sight or establish  radio  communication  (or equivalent) with
the Bridge at all times.

Hypothermia     As   mentioned   earlier   in  this   document,
hypothermia and frostbite are the most  common cold injuries to
humans.  There are great individual differences  in the body's
ability to produce and conserve heat.   When a body's loss  of
heat exceeds  the ability  to produce it, and the  conditions
persist  over  time,   hypothermia  results.    To  prevent
hypothermia, it is  important that the body be  provided with
the energy needed to produce heat.  Energy is provided by food
and drink.   If  you  are cold, eat.  Drink when you  need  to
drink.   Fatigue  is  a real danger.  If cold,  take  steps  to
correct  the  problem.   However,  if  a person  has  failed  to
prevent hypothermia, it is important  that  everyone involved in
sampling activities be capable  of recognizing  the  signs and
symptoms of exposure.  Early  recognition    is    extremely
important.    The victim will often  not  realize  nor  admit
they are experiencing the  signs and  symptoms of hypothermia.

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Some  of  the  symptoms  include  slurred  or slowed  speech,
incoherence, failing memory,  stumbling, and drowsiness.  All
individuals involved in winter  work activities must receive
training   in   regards  to   hypothermia   (and  frostbite).
Attachment  2,  which can  be  used  as a  training guideline,
contains  information  which  includes:  the  definition  of
hypothermia  (and frostbite)  and  associated  risk  factors,
signs  and symptoms  of  exposure,  and  prehospital  treatment
procedures for exposed victims.

Communication  -  Persons  working  on  deck  must remain  in
constant communication with the  Bridge.  Communication can be
established by means of a  two way radio or, when applicable,
the ship's PA system maybe utilized.

When operating in icing conditions, a regular radio watch with
shore und other vessels must be  established  and maintained by
the ship's operator  throughout the icing event.

Water  Tight Doors  - During  an  icing event, all watertight
doors, manholes and  hatch  covers must be battened down.

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Attachment  I

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Attachment II

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Hypothermia

Hypothermia is defined as a spontaneous drop in core body temperature below 95*F (35 *C).
Hypothermia can occur at air temperatures  up to  18.3°C (65°F) or in water up to 22.2°C
(72°F).  All people, including those in excellent physical condition, can become hypothermic
given the necessary environmental conditions and exposure duration.  Heat loss  is increased
greatly  by  low  environmental  temperatures,  low  relative humidity,  and high  winds.
Inappropriately light or porous clothing allows body heat to escape.  Also, clothing made damp
by rain or perspiration loses much of its insulating properties arid allows further heat loss.

Immersion Hypothermia. Because of the very high thermoconductivity of water (25 times that
of air), the body loses heat rapidly when submerged, however, patients with submersion times
of up  to 40  minutes have been successfully resuscitated  with no subsequent  neurologic
impairment.

Risk Factors.  Risk factors for hypothermia include:  alcohol or drug intoxication, receiving
medications such  as barbiturates,  phenothiazine, general anesthetics, reserpine, tricyclic
antidepressants.  Some  medical conditions may also be predisposing factors such as adrenal
insufficiency, diabetes, neurologic, cardiovascular disease with diminished cardiac output, acute
or pre-existing skin conditions.
Signs  and Symptoms

Mild Hypothermia

In mild hypothermia the core temperature is 32° to 35°C or 90° to 958F.

1.      Skin - The skin is often cool to the touch.

2.      Neurologic - Some mildly hypothermic patients are asymptomatic. Otherwise, neurologic
       findings include ataxia, slow gait, incoordination, and dysarthria. Confusion and apathy
       may be apparent.  Deep tendon reflexes  are usually normal or hyperactive.  Pupillary
       reactions are normal.

3.      Cardiovascular - Most patients with mild hypothermia have no significant cardiovascular
       abnormalities.   Decreased or increased heart rate, hypotension, and hypertension have
       variously been reported.

4.      Pulmonary - Respiratory rate is generally normal or slightly decreased.

5.      Musculosketal - Generalized  weakness and  fatigue are common complaints. Shivering
       is usually present.

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Severe Hypothermia

In severe hypothermia the core temperature is below 32°C, 90°F,

1.      Skin - The skin feels cold to the touch and bluish.  Edema may be present.  Signs of
       frostbite may develop if the patient was exposed to subfreezing temperatures.

2.      Neurologic  - Severely   hypothermia  patients always manifest  altered  levels  of
       consciousness, progressing from lethargy and stupor (32* to 27'C)  to  frank  coma
       (<27°C).  Inappropriate and dangerous behavior, such as paradoxical undressing, may
       occur.

       Confusion and hallucinations may be present, making the. patient difficult to manage.
       Pupils become dilated at 29°C (84°F). Deep tendon reflexes diminish between 30°C and
       20°C (86°F and 68°F).  Deep tendon reflexes, voluntary motion, and response to pain
       are  absent at core temperatures below 26°C (79°F).  Corneal reflexes are retained the
       longest and do not disappear until the body reaches 23°C (73°F).

3.      Cardiovascular - Decreased blood pressure and heart rate are common at temperature
       below 32eC  (90°F).  At very low temperatures (<268C or 79°C) pulse and blood
       pressure recordings may be unobtainable.

       Cardiac dysrhythmias become major considerations at temperatures below 30C° (86°F).
       Atrial fibrillation  and  flutter are fairly common.   Premature ventricular contractions
       (PVC's)  are also seen.    Spontaneous  ventricular  fibrillation  is possible at core
       temperatures below 30°C but becomes more likely as temperatures reach 28°C (82eF).
       Ventricular fibrillation and asystole are very likely to occur as the temperature reaches
       22°C (72°F).

4.      Pulmonary - Respiratory  rate  decreases  at core  temperatures  below 32°C (90°F).
       Bronchial secretions are increased in response to the cold, potentially leading to broncho-
       pneumonia and further respiratory impairment.

5.      Musculoskeletal - Severe hypothermia is  characterized by marked weakness, fatigue,
       limitation of movement, and sometimes frank rigidity.  Shivering is usually absent at core
       temperatures below 32 °C.

       In addition to cardiac complications, other important potential metabolic complications
       include aspiration  pneumonia, pancreatitis, decreased renal function, metabolic acidosis,
       respiratory acidosis, hyperglycemia, hypoglycemia, electrolyte disturbances, and rarely
       disseminated intravascular coagulation.

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Treatment

If hypothermia is suspected, the patient's temperature should be taken with a low range rectal
thermometer or probe; capable of measuring temperatures as low as 28°C.  In most instances,
use  of a  low-reading  rectal  probe  (laboratory  thermometer or commercially available
hypothermic thermometer) is recommended.

Prehospital

Prehospital management of the hypothermic patient consists of four parts: (1) basic and advance
life support; (2) prevention of further heat loss; (3) stabilization of associated injuries; and (4)
rapid transport to the hospital.

I.     Basic and Advanced Life Support

              Gentle  Handling.   Rough, jerky  movements and other  stimulation  may
              precipitate ventricular fibrillation in the severely hypothermic patient.  Therefore,
              transfers, movements, and procedures undertaken at all levels of patient care
              should be done as  smoothly and gently as possible.

              Airway.  Establish an adequate airway.  Indications for endotracheal intubation
              include apnea, severe hypoventilation (<6  breaths/rain),  and inability of the
              patient to  protect  the airway from vomitus, blood,  and secretions.   Because
              intubation  may precipitate ventricular fibrillation in  the severely  hypothermic
              patient, it should be done as gently and atraumatically as possible, preferably by
              the  most  experienced person.   Esophageal obturator  airway (EOA)  is  an
              acceptable, though far less desirable, alternative to the endotracheal tube for the
              apneic patient.

              Conscious- patients with good airway reflexes do not require intubation; however,
              they still may need frequent suctioning of secretions.  As in all patients with
              associated  known or suspected trauma,  airway control should be accomplished
              without manipulation  of the cervical spine.

              Fluid Therapy. Volume depletion is often present in the hypothermic patient.
              Although usually secondary  to  plasma volume  depletion  from cold diuresis,
              hypovolemia may also be the result of blood loss from associated trauma.

              An intravenous line should be established in the hypothermic patient for both drug
              and  fluid administration.  Hypotensive (<90mm Hg systolic) patients should
              receive a 300 ml (5 ml/kg) bolus of  isotonic saline (0.9% NaCl) or D5-0.9%
              NaCl.   Patients with  persistent hypotension and evidence of blood loss should
              receive continuous rapid  volume infusion.

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Cardiac Monitoring and Treatment of Dysrhythmia.  Because hypothermic
patients are extremely prone to dysrhythmia, a cardiac monitor should be attached
as soon as possible.   Benign dysrhythmias (atrial fibrillation  and  flutter,
premature  atrial  and  ventricular  contractions,  sinus bradycardia, and  sinus
tachycardia) arc usually corrected with rewarming and, therefore, do not require
treatment in the  field.  Dysrhythmias that result in severely reduced or absent
cardiac output (ventricular tachycardia, ventricular fibrillation, complete heart
block, and asystole) require immediate attention.  Cardiopulmonary resuscitation
(CPR) should be started immediately on the unconscious, apneic patient who has
no  pulse  or  measurable blood   pressure.    Standard  pharmacologic  and
countershock therapy may be initiated with some notable modifications:

       1.     Bradycardia  associated  with   severe  hypothermia  is  usually
             physiologic and   therefore  requires no  treatment  other than
             rewarming. ^'ropine, is usually ineffective.  Isoproterenol, may
             be arrhythmogenic in the hypothermic  patient, and may cause a
             fall in blood pressure and promote heat loss.

       2.     PVC's should be watched closely but  generally do  not  require
             treatment.

       3.     Ventricular fibrillation secondary to hypothermia usually occurs at
             core temperatures below 30°C (86°F).  Treatment  consists  of
             immediate countershock at 200 to 300 joules, followed by a second
             countershock,  should the first be unsuccessful. CPR  is started if
             effective cardiac output is not re-established.  Medications for the
             treatment of ventricular  fibrillation  include:  Lidocaine HC1,  I
             mg/kg bolus, repeat with 0.5 mg/kg after 10 minutes;  begin IV
             drip at 1 to 4 mg/min; bretylium tosylate, 5 to 10 mg/kg bolus;
             epinephrine. .01 mg/kg bolus; and sodium bicarbonate, 1 mEq/kg
             bolus.

             Hypothermic  patients  in  cardiac   arrest   may  respond  to
             pharmacologic and countershock therapy, but it is more likely that
             they will not!  The  patient with a hypothermic myocardium is
             relatively  refractory   to drugs  and  countershock,  and requires
             rewarming before these interventions become effective.  Treating
             the hypothermic cardiac arrest victim  with multiple drug doses and
             countershock is discouraged.  Rather, the major emphasis  should
             be on rapid rewarming.

       4.     Cardiopulmonary resuscitation in the hypothermic cardiac arrest
             victim should be started as soon as possible and continued as long
             as  necessary.  Because of the reduction  in cerebral oxygen

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                           requirements, severely hypothermia patients (particularly children)
                           can   survive  several   hours   of  resuscitation   and   remain
                           neurologically intact. Under no circumstances should resuscitation
                           be discontinued in the  field:  rewarming to at least 32"C (90°F')
                           must be done at the hospital before discontinuing resuscitation.

                     5.     Other medications that can  be administered are  thiamine and
                           D50W,  and naloxone.   Thiamine  and D50W are  given because
                           many hypothermic patients are also hvpoglvcemic:  50 ml of 50%
                           dextrose (D50W)  may given  intravenously to those patients with
                           altered  levels of consciousness.   A  blood sample should  be
                           obtained prior to administration of the dextrose so that laboratory
                           confirmation of hypoglycemia may be made later. Dextrose given
                           to  the   alcoholic  hypoglycemic, patient   rarely  precipitates
                           Wernicke's  syndrome.    It is  prevented by  giving a  dose  of
                           thiamine,  100 mg IV, before  giving the dextrose.   Patients with
                           altered levels of consciousness in whom you suspect narcotic use
                           may receive naloxone.  The dose is 0.8 mg (2 ampules) for adults
                           and  0.4  mg (1  ampule)  for  children;  both   administered
                           intervenously.

II.     Prevention of Further Heat Loss

       The prehospital provider should take measures to ensure that the hypothermic patient
       does not become more hypothermic in transit.  This precaution is distinct from actual
       rewarming,  which is not a  primary goal during  prehospital care.  In fact,  many
       investigators recommend keeping the patient cool during transport.

       The activity level of the patient is kept to a minimum.  Wet clothing should be removed
       and replaced with dry clothes and blankets.  Areas of the body that lose large amounts
       of heat, such as the head, neck, and trunk, especially,  should be covered.  Sips of warm
       fluids may be given by mouth, but only to the  awake, alert patient.  Alcoholic beverages
       are absolutely contraindicated.

       The only active rewarming method recommended for  field  use is warm humidified
       oxygen. Oxygen heated to  40 to 45 °C  (104 to 113°F) and administered to the patient
       via  face mask or  endotracbeal  tube supplies a small  amount of core heat to the
       respiratory tree.  It also reduces  heat  lost through ventilation.  Although not widely
       available, a portable device for heating oxygen in the filed has been described by Lloyd.
       Soda lime and water are the heat sources.

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III.    Stabilization of Associated Injuries

       Appropriate immobilization of the cervical spine is mandatory for patients with known
       or suspected head and neck trauma and who have altered levels of consciousness. Near-
       drowning   victims  often have   preceding   head  and  neck  trauma   resulting  in
       unconsciousness, paralysis, aspiration, and hypothermia.

       Immobil'zation of the cervical spine  is best accomplished using a backboard, a rigid
       cervical collar, and sandbags.

       Splinting of injured extremities may be done in the field if the patient is stable and if the
       time permits.  Frostbitten areas should be padded and then loosely wrapped to protect
       them from further injury.  Thawing of frostbitten areas generally should not be attempted
       prior to arrival at the hospital!                            '
IV.    Rapid Transport to the Hospital

       The definitive treatment for the hypothermic patient is rewarming. Because it usually
       cannot be done safely in the field, efforts should be made to transport the patient to the
       hospital as rapidly as possible.

       Hypothermic patients in cardiac arrest should receive no more than one course of drugs
       and two courses of countershock therapy in the field. Prolonged prehospital resuscitation
       are generally futile and sometimes detrimental. With CPR in progress, patients should
       be transported to the hospital, preferably  one where cardiopulmonary bypass procedures
       can be performed if needed.  The hospital should be notified prior to arrival so that he
       "pump team" and equipment can be readied.

       Rewarming Techniques

       Definitive therapy for most cases of hypothermia involves rewarming the body to near
       normal temperature. Rewarming techniques include passive external, active external, and
       active core rewarming.

             Passive External  Rewarming.    This method  allows patients to  rewarm
             themselves without the application of external or internal heat sources.  Passive
             external rewarming,  although  rather  slow,  is  safe  and effective  for most
             hemodynamically stable patients with mild hypothermia.

             By combining the effects of reduced heat loss  and endogenous heat  gain, core
             temperature should increase at a rate of approximately l°C/hr using passive
             external rewarming. The following  procedures are recommended: (1) remove
             the patient's cold  or wet clothing and dry the skin  with towels;  (2) place the
             patient in a warm room, away  from wind and  drafts; and (3) cover  the patient
             with blankets or other insulating material.

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              Active External Rewarming.  Rewarming the patient by applying a heat source
              to the body surface is an old,  albeit controversial, technique.  Although it  is a
              rapid method of rewarming, many clinicians recommend against its u se because
              of potential hypotension from active external rewarming, known as "rewarming
              shock" and possible ventricular fibrillation.

              Still,  there are  proponents  of active external  rewarming  techniques,   they
              recommend these methods primarily for patients with hypothermia of short-term
              duration (e.g., exposure, immersion) whose cardiovascular systems can tolerate
              the added stress.  Also, patients undergoing active rewarming must be closely
              monitored for dysrhythmias and  hypotension and have  adequate intravenous
              access established for fluid replacement in the event that hypotension occurs.

              The following are active external rewarming techniques:

              1.     Heated blanket,  hyperthermic mattresses  ,
              2.     Hot water bottles placed at  areas of rapid heat exchange  (e.g., neck,
                     flanks, axillae, and groin)
              3.     Immersion  in  warm  water bath  (40°C,  104°F).    Active  external
                     rewarming is not recommended in the field!
Prevention
Cold injuries can best be prevented by wearing warm, lose, dry clothing (designed to resist wind
and rain) in multiple layers on the head, body, and extremities to prevent heat loss.  One should
keep dry at all costs; materials  lose insulation properties when wet.  Wet garments should be
replaced as soon as possible with dry one, and constrictive garments should not be worn.

Jobs should  be designed so that workers  remain  relatively  active  when  exposed to cold
environments and provided  with dry,  wind-protected, heated  shelters  for tasks  involving
stationary work positions.

Workers exposed to  the cold should be physically fit, without underlying  vascular, metabolic,
or neurologic diseases that  place them  at increased risk for hypothermia.  They should be
cautioned to  avoid smoking and drug or alcohol use.  New workers should be introduced into
the work schedule slowly and instructed in the use of protective clothing,  recognition of
impending frostbite and early signs and symptoms of hypothermia, proper warming  procedures,
and first-aid  treatment.

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 Frostbite

 Frostbite is caused by exposure to temperatures below freezing, causing ice crystal to form in
 tissues. A combination of high winds and cold is much more dangerous than cold alone.

 Predisposing Factors:

              Subfreezing temperature 20°F (-7°C)
              Wind-chill factor
              Alcohol and drug intoxication
              Underlying illness, diabetes, peripheral  vascuiar disease
              High altitude
              Conduction injury (e.g., frozen metal object)
              Fatigue
              Previous history of frostbite iiijary
              Tocacco use

 Signs and Symptoms

 Frostbite occurs most often on distal and exposed body pans. Fingers, toes, cheeks, nose, and
 ears are common sites.  In mild cases with superficial freezing(frostnipj pain, pricking, itching
 sensation and numbness will occur without complete loss of sensation.

 In more severe deep  frostbite skin is white, feels firms, edematous, immobile, no sensation or
 pain.

 Treatment

 Rapid thawing of the frozen body part by immersion in warm water is the treatment of choice.
 methods that are Qgntraindicated aie hot-or-cold water immersion, and rubbing the frozen body
 pan with ice or snow.

 In the Field

Rewarming may be attempted for frostnip. Immersion in warm water (temperatures of 38 to
43°C or 100 to 1108F) is the preferred method.  An alternative method is direct contact with
one's own skin or with a companion's skin.

In cases of severe frostbite, hospital ization is recommended. Extremities can be re warmed by
removing wet gloves, socks and shoes; drying the extremities and covering them again with dry
clothing; and either elevating them or placing them next to a warmer part of the body (e.g.,
placing the  hands in the armpits).  Caution:  Rewarming should not be attempted if refreezing
 is likely prior to definitive therapy.

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Rewarding of the frostbitten parts of the body can be accompVshed by placing them in> water
heated to 40 to 42°C and leaving them there until thawing is complete but no longer (often 30
minutes)  Dry heat is not recommended. Frostbitten parts should not be exercised, rubbed  or
exposed to pressure.  Dressings and bandages should not be applied. Great care should be taken
to avoid any trauma to the extremity during and after thawing.  For example  the extremity
should not touch the sides or bottom of the rewarming container. Gentle manual or mechanical
circulation of the water helps the thaw to be more uniform.

The goal of Held management is to protect the frostbitten area  from further injury followed by
rapid evacuation to a medical facility.

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Attachment III

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     My signature at the bottom of the second page indicates my
understanding and concurrence with the following requirements.


                             RELEASE

     In consideration for being allowed to board the Research
Vessel Lake Guardian on this 	day of	, 19	, I agree that,
while on board the vessel, I will follow all safety instructions
and emergency procedures which I have been given and those which
I will be given; and I, on behalf of myself, my personal
representatives, assigns, heirs, and next of kin, hereby release
and discharge and covenant not to sue the United States, its
agents and its employees (hereinafter referred to as "releasees")
with respect to any liability to me, my personal representatives,
assigns, heirs, and next of kin, relating to any injury, loss, or
damage incurred by me while on board the Lake Guardian, whether
caused by the negligence of the Releasees or by other causes.

     I represent that I have been given comprehensive
instructions on safety and on emergency procedures, and have been
informed that I will be restricted from entering hazardous areas
of the vessel.  I understand that my safety will depend upon my
following all such instructions and restrictions while on board
the vessel.  I understand further that, because of the potential
for having to don floatation gear in the event of an emergency,
all persons who board the vessel must be at least nineteen years
of age and in good physical condition.
                    INFORMED CONSENT ADDENDUM

The R/V Lake Guardian sustained collision damage November 26,
1994, consisting of a six inch split in the hull plates at the
bow of the vessel, which punctured the forepeak tank,
approximately two and a half feet above the water line. This
damage has been inspected by the EPA safety Manager, the U.S.
Coast Guard, and the American Bureau of Shipping. Repairs,
consisting of removing the split steel plate and welding a
replacement steel plate in place of the split, have been made.
the forepeak tank is water tight and the vessel has been
certified as seaworthy.

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                    WINTER OPERATIONS  ADDENDUM

Persona boarding the vessel during winter operations (November
through March) should be aware of the possibility of below
freezing air temperatures, strong winds and icy decks, and should
bring adequate protective clothing to prevent frost bite while
working outdoors.

Safety rules will be amended to take consideration of hypothermia
due to 0°-3° lake water temperature,  deck and superstructure icing
conditions, or the approach of ice fields. Survey activity will
be aborted in favor of safe ship operations. Schedules may be
interrupted for several days due to weather.

Violation of safety rules will result in the, offender being put
ashore at th9 first port of opportunity.
        ACKNOWLEDGEMENT OF RECEIPT OF SAFETY INSTRUCTIONS

     I represent that, prior to boarding the Research  Vessel
Lake Guardian, I was given comprehensive instructions on safety
and on emergency procedures, and was informed that I will be
restricted from entering hazardous areas of the vessel.  I
understand that my safety will depend upon my following all such
instructions and restrictions while on board the vessel.  I
understand further that, because of the potential for having to
don floatation gear in the event of an emergency, all persons who
board the vessel must be at least nineteen years of age and in
good physical condition.

     In consideration for being allowed to board the Research
Vessel Lake Guardian on this 	day of 	, 19	, I agree
that, while on board the vessel, I will follow all safety
instructions and emergency procedures which I have been given and
those which I will be given.
               First,  Middle,   Last Name

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          STANDARD OPERATING PROCEDURE (NO. 6-REVISED)
                     FUELING TRANSFER PROCEDURE

1.0 SCOPE

This procedure shall be followed each time a transfer of fuel oil takes place.

2.0 REFERENCES

Code of Federal Regulations Titles 33 & 46

3.0 RESPONSIBILITY AND AUTHORITY

The ship's Chief Engineer, acting under the authority of the Master, shall be responsible
for performance of the procedures describe^ here-in.

4.0 EQUIPMENT REQUIRED

Included in procedure.

5.0 PROCEDURE

5,1    Ship To Shore Transfer Procedures

1.      There will be no visitors, smoking,  fires, open flames, open lights, no grinding and
       no burning or welding while fuel is being transferred .  A sign will be posted and an
       announcement will be made over the vessel's intercom system to inform personnel.

2.      All power tools and other equipment that could spark, i.e. drills, saws, etc., will be
       secured.

3.      Before transferring fuel oil.

a.      Check gaskets in all fittings and replace those suspected to be damaged.
b      Check the fittings themselves to insure they are not cracked or stripped.
c      Examine all hoses and make sure they are in good working order.  Look for tears,
       frays, kinks or other weak spots that might cause the hose to burst or leak.
d      Display the "Bravo" flag by day, or an all around red light by night.
e      Place  oil soak pads and 5 gallon buckets by all fittings.  Plug containment bins.
       Be prepared  to transfer containment bin  contents to a suitable container for
       disposal, should a leak occur.
f.      All "cam lock" fittings must be securely closed with all tabs fully engaged.  Wire
       tabs in place  to prevent accidental openings.
g      Check all mooring lines to make sure they are secure.

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h.     Sound all tanks using water paste. Log the soundings and the draft of the vessel
       prior to and after the transfer.
i.      Establish good communications with the pumping station via VHP radios.
j       When  ever possible,  the hoses should be air tested by the  shore station as to
       the integrity of the seals.
k.     Ensure that all involved personnel are at their appropriate stations. Chief
       engineer will man the fuel transfer manifold in the engine room.  The assistant
       engineer and marine electrician will be positioned at the port and stbd
       sounding wells. One seaman  will stand by the transfer connection and one will
       standby in  the pilot house. All of the above people will have radios and will be in
       communication with the Chief in the engine room
1.      The  appropriate amount of Drew Chemical Amerstat  10 Fuel Oil Treatment
       should added to the fuel tanks  prior to transfer.  A maintenance dose of 13 oz per
       1000 gal of fuel added via the sounding tubes will suffice.
m.     If loading  by tanker  truck, make sure they have permission from  th; USCG
       Captain of  the Port to transfer fuel at that specific location.

4.     When the above procedures have been completed, and all necessary valves have
       been opened ( see number 6 of this section), transfer may commence.  During
       the pumping process, persons will not leave their station unless relief is approved
       by the Chief.  Persons should be on the look out for people in violation of the
       open flame restriction.  Have  soundings made as often as needed. As the tanks
       approach 80% capacity, slow transfer and stop at 90%.

       Note: In accordance with 33 CFR 156.150, whenever oil or  hazardous materials
       are transferred to or from a vessel, a declaration of inspection form must be filled
       out and signed prior to the transfer procedure This form must be maintained on
       board for a period of one month.

5.     Upon  the  completion of the  transfer, ensure that all valves used  during  the
       transfer are properly  closed.  It is the responsibility of the Chief Engineer to
       double check that  these valves are indeed closed.   Whenever possible the hoses
       should be blown down or sucked back to prevent any spillage  during disconnect

6.     The following procedure shall be used for opening and closing valves:
       Note: The following assumes that all valves are closed prior to transfer.

       a.     Open fuel oil filling valve.
       b.     Open manifold filling valve.
       c.     Open both port and stbd filling valves for which ever tanks are to be filled
              first (1's, 2'sor3's).
       d.     Open deck  valve.
       e      To  secure, close in reverse order.

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       Never close both port and stbd fill valves when fuel oil is pumping.  Hose blow
       out could occur  Always open the next tank before closing the previous tank

5.2    Tank to Tank Transfer Procedure

5.2.1   Any  time fuel oil is transferred between two storage tanks (excluding the day
       tanks), the following procedures will be followed:

       1.      The Chief Engineer will be in the engine room controlling the  manifold
              valves.  Two Engineers will be responsible for sounding the tanks which
              are being transferred.

       2.      All personnel will be in communication via radios.

       3      All 3pill precautions from the fueling SOP stated above will be followed.

5.2.2   When fuel is transferred to the day tanks, the following procedure will be followed.

       1      The Chief Engineer, or  his designee, will be responsible for the transfer of
              fuel oil to the Day Tanks. No other persons are needed.

       2.      At no time during transfer procedures shall transfer personnel engage in
              any other activities  Additionally, at no time should level indicator alarms
              be disconnected/silenced

       3.      Fuel will be pumped from the storage tanks to the day tanks vis the transfer
              pumps   The fuel  will  pass through  a pre-filter and a water separator,
              then through a fuel oil meter, and finally into the day tanks.

       4      A constant watch of the tank level indicator for the day tank will  be made.
              At no  time should the level go above 10.  .

       5      When transfer is complete, the  valves should be  returned to their closed
              position
6.0 DISCHARGE CLEAN-UP

In the unfortunate event that oil or hazardous material from any sources is discharge into the
water or upon the adjoining areas during transfer procedures,  transfer shall immediately
stopped.  No person may resume transfer operations until the discharge is stopped, contained
and the area has been cleaned up

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7.0 PROCEDURES FOR REPORTING OIL SPILLS

The Federal Water Pollution Control Act prohibits the discharge of oil or oily waste into or
upon the navigable waters of the United States or the waters of the contiguous zone if such
discharge causes a film or sheen upon or a discoloration of the surface of the water or causes
a sludge or emulsion beneath the surface of the water.  The law requires that all discharges
be reported to the US. Coast Guard. Fines for non-compliance with the law can result in
civil and  criminal penalties.

8.0 VESSEL SCHEMATIC

Attached is a schematic diagram of the R/V Lake Guardian's fuel oil piping system.

9.0 PRODUCT LISTINGS

Number 2 Marine Diesel Fuel Oil
Drew Chemical Amerstat 10 Fuel Oil Treatment

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                            OF run. on. TRANSIER  SYSTFM
     fun. Ot FROM ANY TANK CAN  BE  TRANSTWnH) TOSHORE AND VICE
     THROUGH THE nitL OIL MANIFOLD TO THE FILL /DISCHARGE CONNECTIONS
     LOCATED AT FR 8« ON MAN DECK STBO AND FR 97 ON  MAW DECK PORT SIDE.
     THE FUEL TRANSFER WlF LOCATED N  TIC ENGINE ROOM STBO SIDE
     B intD TO TRANSFER FUEL.  A STANDBY FUFL OK.  TRANSFER  PUMP B ALSO
     PROVIDED   OIL SPILL  CONTAINERS ARE INSTALLED UNDER  THE  FIL/TJISCH.
     CONNECTIONS AND All- FUEL TA»IX VENTS (OVERaOWS) TO COLLECT ANY SPlLS
     OR OVERFLOWS FROM  THE FUEL TANKS DURING FUELING OR DEFUEUNO OPERATIONS .
     M CASE or STILLS INTO THESE CONTAINERS THE OIL IS REMOVED BY MANUAL MEANS
     AND P*T») INTO THE DIRTY CC WK.  - LOCATED  PORT  SIDE  ER 70-74  N THE
     MNCT BOTTOM  t4  HIC ENGINE ROOM.

     IOCATIONS OT TANK OVERELOWS ANO OIL SPILL CONTAINERS ARE AS FOLLOWS)
     ER SJ -83  STBO StOK  MAIN  DECK ------- MO.  1 * 1 FUFL OIL TANKS
     rn  *7 STUD s»* IUJN DECK ---------- NO s FUEL on TAMK
     FR  «0 STOO S1TTE MAW DFCK-- • ------ STBD FUTL OIL DAY TANK
     FR  84-SS PORT  SIDf MAIM DECX ------ HO. 1,1 * 3 HFV  OIL TANK
                                         ANO PORT FUFL Oft DAY TANK
                  FUEL 0*. FRAN"'-1 PROCEDURE

NOTE-  BEFORF FUDINO OPERATIONS BEWH. SECURE THE VESSEL Tel THE DOCK OR OTHER
      VESSEL WTTH BOW. STERN ANO SrWNS IIHES TO PrtFVFNT SHIFTING  DURING  FUEUNO.

1   THE MASTER OR A DESIGNATED  OFFICER SHALL  BE  W THE PHOT  HOUSE DURING
   FUELKo OPERATIONS.   STATION THE  CHIEF FNCINEER AT  THE  MAIN FUEUNO STATION
   LOCATED W Tl« DK3NE ROOM  AT THE FUEL OIL MANroLD.   STATION ONE ENGINEER
   AT THE UAIN DECK FH1/DBCM. STATK3H PORT  Cfl  STBO  DEPENDING OH WHKH SIDE
   SUP B FtECEMMO OR DISCHARGING  FUH.  ASSIGN ONE MAN TO SOUND  FUEL TANKS
   ON POffT SIDE ANO ONE  MAN TO SOUND TANKS ON STBO SIW.  AIL PERSONNEL
   SHALL BT W COMMUNICATION   BY SOUND POWPED PHONES.  THE ENGINEER AT
   THE mi^DGCH STATION Will BE IN COMMUNICATIONS WITH  THE SHORE F«1LTrY.


                                       FUEL OIL DAY TK  (p)

                                        -REMOTE OP. FROM MN DECK(MANUAL)
                                                 (NO.)
     CHIEF ENCWEER NSORt AU VM.VCS  M THE FUEL SYSTEM AW CLOSED EXCEPT
     FOR VALVES MARKED NORMAU.Y OPEN.  OPCN VM.VC ON  MMtPOLO TO PUB. TANK
     TO K HLLED OR SUCTION TAKEN FROM.                               ,
                                                                                                                                                   ENCMEBt
,    RFUOVE BLNO FLANOC FROM nLL/BtSCH CONM. ANO ATTACH FUEL HOSE.
     RECEIVES PERMISSION FROM CHEF ENGWEER ANO NOmS THE SHORE
     TO COMMENCE fUCUNOL

4.  !  MAN SOUNOtM TANK BEtM FtlED OR EUPTE) SOUNDS TANK AT FREQUENT MTER-
     VM3 ANO REPORTS  WHEN TANK 8 to* FUU OR WHEN TANK B EMPTY. CHEF ENO.
     THEN SHm TO ANOTHER TANK BY OPEMNO ANO CLOSMO REOO,  VALVtl ON UAMFOUL

J.  .  DURMO FUEtJNO THE ENCMEER ANO MEN SOUNPtM TANKS SHALL KEEP A CLOSE
     VISUAL CHECK ON THE SPUL OONTAMERS.  f AN OVERFLOW OCCURS  HE SHMi.
     MJEDWELY NOJIfY  THE  CHEf ENO. TO SHFT tANKS OR  SECURE THE FtBL  VjJjVE
     AT THE FUL/DSCH.  CONN.                                                   :

«.   ' WHEN All. TANKS ARC TOPPED OFF OR EMPTIED, THE ENONEER AT THE FLL/MSCH.
     CONNECTION B TO NOTTY THE SHORE FAOt/IY TO  SECURE FUELMa

7.    REMOVE FUEL  HOSE  AT THE FU/WSCHAROE CONNECTION  AND PfFtMX BUND
     FLANGE, U9NO CAUTION TO FWVENT  SPliS FROM  FUEL REMAMM M  THE HOSE.

g.    SECURE VMVCS M THE FUEL SYSTEM EXCEPT THOSE THAT ARE NORMALLY OPEN.


     NOTEl  AW Ot SPILS inOTHE WATER SHAU BE  UUEDMTELY REPORTED TO THE
          MASTER Of THE VESSEL.
                                                                                                   rF.O. F1LL/DBCH.
                                                                                                         rOlFTTY Olt OTSCH CONN,
                                                                                                             . SPV.L CONDtlNER
  TO  F.a FILL/OI5CH.  COMNECTIOff  -
               (OETAILV)
                                                                                                 DETAA.V
                                                                                                              IMNS FUEL OH. TK
SYMBOLS
M
m
•

©
*-
e
-*-
N.O.
t-
BUTTERFLY VULVE
6ATE VALVE
SWPLEX BASKET STRAJNtR.
FUEL OH. SEPARATOR
FUEL OIL PREFTLTER
PIPE TURNED DOWN
Plft TURNED UP
FLOW DIRECTION
NORMALLY OPEN
PIPE CAT
             FUEL OIL MANIFOI 0 —  —
             DISCH. f> TOP
             5UCT.  « BOTTOM
A' ft) OIRTr OIL
  DISCH CONN
                 REMOTE OP. FROM MM  DECK
                          (N.O.)
                                           [[_rUEL 01L  FILL A TRANSFER 01AGRAM11
                                               USEP* R/v "-*** 4UAMNMI
                                               FUEL CM.  TRANSFER PROCEDURES
                                                                                                                                    DATE'  4 AU6 i

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                                R/V LAKE GUARDIAN
 STANDARD OPERATING PROCEDURE FOR THE HVAC MAINTENANCE SYSTEM
                                      MAY 1997
INTRODUCTION

These Standard Operating Procedures (SOPs) were developed to comply with  the Heating
Ventilation, and Air Condition (HVAC) requirements noted in the U. S. Environmental Protection
Agency (USEPA) Facility Safety Manual and the USEPA Safety, Health and environmental Audit
Protocol.

FILTER REPLACEMENT

General Requirements
To Maintain acceptable indoor air quality conditions on board the R/V Lake Guardian, all air filters
associated with the HVAC system must be cleaned and replaced on a regular basis in accordance with
EPA  4870, FS1-05 (12).  To comply with this requirement,  the following schedule has been
developed FOR the cleaning and replacing of HVAC filters on board the Lake Guardian:

       1.      All air filters associated with the R/V Lake  Guardian  HVAC system should be
              inspected at least quarterly during periods of regular use by qualified personnel.
              At that time, air filters should be cleaned as needed.  In addition, filters that appear
              to be worn or broken should be replaced.

       2.      All air filters associated with  the R/V Lake Guardian HVAC system shall be
              replaced, at a minimum, on an annual basis.

Inspection Requirements
Inspection requirements, task assignments, and record keeping documents for the maintenance of the
ship's HVAC system are maintained/generated by a computerized Preventive
maintenance  System referred to as "MAINTAINIT".  The system automatically generates work
assignments (Task Reports) based on parameters previously entered by the user. The Task Report
include equipment description, inspection frequency, equipment location, and task instructions.  The
Chief Engineer is responsible for tbe maintaining the program, and making revisions when necessary.
Additionally, the Chief Engineer is responsible for generating Task Reports, assigning the task, and
recordkeeping. Inspections procures shall be conducted in accordance with Task Instructions found
within the task report (see attachment for Task Report).

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HVAC DUCT AND AIR HANDLER CLEANING

General Requirements
To Maintain acceptable indoor air quality conditions on board the R/V Lake Guardian, all air handling
units and HVAC duct systems on board the Lake Guardian must be cleaned on a regular basis in
accordance with EPA 4870, FS1-05 (12). To comply with this requirement, the following schedule
has been developed.

       1.     All air handlers and duct work associated with the R/V Lake  Guardian HVAC
             system should be  inspected at least quarterly during periods of regular use by
             qualified personnel.

Inspection Requirements
Inspection requirements, task assignments, and record keeping documents FOR the maintenance of
the ship's HVAC system are maintained/generated by a computerized Preventive Maintenance System
referred to as "MAINTAINIT".   The system automatically generates work assignments (Task
Reports) based on information previously entered by the user (ie. The Chief Engineer). The Task
Report  include  equipment  description, inspection frequency,  equipment  location,  and  task
instructions.   The  Chief Engineer is responsible for the maintaining the program,  and making
revisions  when necessary.  Additionally, the Chief Engineer is responsible for generating Task
Reports,  assigning the task, and  recordkeeping.  Inspections procures  shall  be conducted  in
accordance with Task Instructions found within the task report (see attachment for Task Report).

HVAC MECHANICAL SYSTEMS

General Requirements
To Maintain acceptable indoor air quality conditions on board the R/V Lake Guardian, all Mechanical
components  associated with the  HVAC system  on board the Lake Guardian must  be regularly
inspected and maintained.  To comply with this requirement, the following schedule has been
developed:

       1.     On a quarterly basis evaporators shall be cleaned and inspected

       2.     On a quarterly basis condensers shall be inspected and cleaned

       3.    On a quarterly basis motors shall be inspected and lubricated

       4.     On a quarterly basis belts shall be inspected and replaced as needed

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Inspection Requirements
 Inspection requirements, task assignments, and record keeping documents for the maintenance of
the ship's HVAC system are maintained/generated by a computerized Preventive Maintenance System
referred to as "MAINTAINIT"  The system automatically generates work assignments (Task
Reports) based on parameters previously entered by the user (ie. The Chief Engineer). The Task
Report  include  equipment description,  inspection  frequency,  equipment location, and  task
instructions.   The Chief Engineer is responsible for the maintaining the program, and making
revisions when necessary.  Additionally, the Chief Engineer is responsible for generating Task
Reports, assigning the task, and recordkeeping.  Inspections procures  shall be  conducted in
accordance with Task Instructions found within the task report (see attachment for Task Report).

PERIODIC PERFORMANCE TESTING/INDOOR AIR QUALITY SURVEY

General Requirements
Ventilation is one of the must critical factors affecting indoor air quality. Other factors are  physical
(e.g. dusts), chemical (e.g. cleaners), and biological (e.g. fungus/molds) materials present in a facility.
ASHRAE  Guidelines  and EPA Policy Requirements have been  established as  a  basis FOR
performance testing of the ventilation system on board the R/V Lake Guardian to ensure a minimum
of indoor air quality problems.

Monitoring Requirements
On an annual basis, the following specifications for indoor air quality should be measured and
reported by personnel under the direction of a Certified Industrial Hygienist (CIH):

       1.      Carbon Monoxide
                    A.     Shops and industrial areas -18 ppm
                    B.     Offices and laboratories - 9 ppm
                           [EPA4870, FS1-05(12)]
       2.      Carbon Dioxide levels should not exceed 1000 ppm [EPA 4870, FS1-05 (12)]

       3.      Formaldehyde
                    A.     Shops and industrial areas - 0.5 ppm
                    B.     Offices and laboratories -0.1 ppm
                           [EPA 4870, FS1-05 (12)]

       4.      Temperature and Humidity
                    A.     Summer - Maximum 78 degrees F and 60% RH
                    B.     Minimum - 68 degrees F and 30% RH
                           [ASHRAE 62-1989]
Inspections
Inspection activities and monitoring results associated with the above requirements should be noted
during the Annual Industrial Hygiene Survey Report for the R/V Lake Guardian.

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                        STANDARD OPERATING PROCEDURE
Section




1.0




2.0




3.0




4.0




5.0




6.0




7.0




8.0




9.0




10.0




11.0




12.0




13.0




14.0
Small Boat Operational Safety




      Table of Contents




    Title




    Scope




    References




    Responsibility




    Frequency of Inspection




    Preparation and Weather




    Boarding




    Outboard Motor Operation




    Fueling




    Rules of the Road




    Mooring




    Ground Tackle




    Anchoring




    Personal Protective Gear




    Emergency Procedures
Page
Prepared by







Approved by
                       Date
                       Date
Rev.

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                               Small Boat Operational Safety
1.0    Scope
The scope of this manual is to provide  standard operating procedures for the safe navigation,
handling,  maintenance and operation of  small boats, inflatable craft and  related marine science
equipment.
2.0    References

Environmental Protection Agency, Region 5: Health & Safety Manual. Section 18-2.

North Pacific Fishing Vessel Owner's Association:  Vessel Safety Manyal, 1986.

Outboard Marine Corporation: Owner's/Operator's Manual

Avon Inflatables Inc.: Owner's Manual

3.0    Responsibility

The operator in command of any small craft is ultimately responsible  for the safe operation of the
vessel  and it's equipment,  and for the safety of everyone aboard.  As well, the vessel owner must
provide a seaworthy vessel. However, each occupant must attend constantly to his own safety and
perform his work in a safe and prudent manner, using all safeguards provided for his protection or
the protection of others.

4.0    Frequency of Inspection

Prior to any small craft being taken out, it should be inspected to insure that mechanical functions,
such as the motor, lights, radio, etc., are operating, that any necessary documentation is on board,
that standard safety equipment is on board and that there is sufficient fuel.

5.0    Preparation and Weather

Before leaving on your trip, get the weather forecast.  Look at the charts (and tide tables) and plan
your route. Collect and weigh the passengers personal gear, the sampling apparatus and all other
"stores" going aboard.  Load the gear so that it will not roll or slide when the boat rocks or tosses
Before leaving on your trip, you should take the following precautions:

       •      If the weather forecast is  somewhat unfavorable, load the boat lightly so that it will
              float high in the water.  If a storm is predicted,  postpone the trip.

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       •       If your course takes you out sight of land, at least one person aboard should know
              how to navigate.  At least two people should know how to read charts and locate
              sandbars, shallows, rocks, rapids, etc..

              The  Coast Guard recommends that you file a "float plan" with  someone who can
              request a search if you do not return as expected.  The float plan should give a
              detailed description of the boat and its planned course.  It should list the people
              aboard, the type and frequencies of the emergency radio, and the names and telephone
              numbers of the Coast Guard or other agency personnel who should be notified if you
              fail to return as expected.

In addition, before departure, check for local storm warnings. Your charts will show the location of
storm warning display stations, and your radio will carry weather bulletins. A discussions of storm
warnings follows:

       •       A small craft warning means winds may reach 38 mph (33 knots) or conditions are
              dangerous for the sort  of boats generally used by EPA.  A triangular red pennant,
              displayed at a storm warning disphy station, signals a small craft warning.

              Two triangular red flags signal a gale warning: winds from 39  to 54 mph (34-47
              knots).

       •       A  single  square red flag with a black center displayed at a storm warning display
              station signals a storm warning: winds of 55 to 73 mph (48-63 knots).

       •       Two square red flags with black centers signals a hurricane or tropical cyclone, with
              winds in excess of 74 mph (63 knots).

6.0    Boarding

       A.     When your board a small boat, grasp both sides of the boat and then step into the
              middle.  If you put  all your weight on the side nearest the dock, you may push that
              side under water.  If you step down and push the boat away, the boat may move away
              before you are in, (it helps to have someone steady the boat while you step in).

       B.     When one person is in the boat, a second person can pass gear and supplies to him or
              her.  If the cargo being  passed over is heavy, be careful not to tip the boat and lose
              your balance. Distribute the weight evenly, and secure all cargo so it cannot move
              when the boat rocks.

       C.     On larger boats, the load capacity should be displayed on  a plate mounted in the boat
              A certificate of compliance for an outboard motorboat will show the manufacturer's
              rating of maximum horsepower that can be safely used on the boat's engine.

       D.     A  combination capacity plate will also list the  maximum number and  weight  of
              persons that can be carried safely, as well as the maximum weight (of persons, motor
              and gear) that can be carried safely. The certificate of compliance and the capacity
              may be shown on separate plates.

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7.0    Outboard Motor Operation

The operator of any small boat is responsible for the correct operation of the boat and for the safety
of its occupants. All operators should read the Evinrude Outboards Manual and be familiar with its
contents before operating the boat. Section 7.0 of this SOP covers but a small portion of the manual
and pertains solely to the starting, stopping and basic operation of outboard motors.  The Evinrude
Outboards Manual can be found in its entirety in the Ship's Equipment ringbinder.  Copies  of this are
located in the Captain's office, the Lab. office and the Chief Engineer's room.

       A.     Starting and Operation

       1)     During the initial operation of a new motor, the operator must follow the "Engine
              Break-In"  procedure as described in the General Information section of the
              Evinrude Outboards Manual.

*note:  Failure to follow the "Engine Break-In" procedure can result in serious engine damage.

       2)     Place fuel tank in boat so tank will not shift around. Be sure fuel line is not wedged
              under tank.   Allow fuel line slack to permit steering.

*note:  Do not operate motor out of water even momentarily. Water pump  may be damaged or
motor may overheat.

8.0

When handling fuel and refueling remember that gasoline is very flammable. Certain precautions must
be carefully and completely observed every time that a boat is fueled with gasoline.

       Before Fueling

       A.     Make sure that your boat is secured to the fueling pier. Fuel before dark, if possible.
       B.     Stop engines, motors,  fans and other devices that can produce a spark.  Open the
              master switch if the electrical system has one. Put out all galley fires and open flames.
       C.     Close all ports, windows, doors and hatches so that fumes cannot blow aboard and
              below.
       D.     Disembark all passengers and any crew member not needed for the fueling operation.
       E.     Prohibit all smoking on board and nearby.
       F.     Have a checked fire extinguisher close at hand.
       G.     Measure the fuel in the tanks and do not order more than the tank will hold; allow for
              expansion.

       While Fueling

       A.     Keep nozzle or can spout in contact with the fill opening to guard against static sparks.
       B.     Do not spill gasoline.
       C.     Do not overfill.  Filling a tank until fuel flows from  the vents is dangerous.
       D.     For outboards, remove portable tanks from boat and fill on shore.

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       After Fueling

       A.     Close fill openings.
       B.     Wipe up any spilled gasoline; dispose of wipe-up rags on shore.
       C.     Open all ports, windows, doors and hatches; turn on bilge power.  Ventilate boat at
              least four minutes.
       D.     Snifflow down in tank and engine compartments.  If any odor of gasoline is present,
              do not start engine; continue ventilation actions until odor can no longer be detected
              Check for any drips and liquid fuel.
       E.     Be prepared to cast off lines as soon as engine starts; get clear of pier quickly.

Inboard engines require  special ventilation equipment. Use it.  Boats with enclosed spaces where
spilled fuel or fuel vapors can accumulate must have powered ventilation to clear away the fuel
vapors. This requirements does not normally apply to open boats.

9.0    Rules of the Road

       A.     Every vessel must at all times maintain a proper lookout by sight and hearing as well
              as by all other available and appropriate means, to keep fully appraised of the situation
              and any risk of collision.

       B.     Under normal circumstances, the helmsman will satisfy this need, but he must  be
              qualified,  alert and have no other responsibilities.  The use of an automatic steering
              mechanism and/or the use of radar at night or in fog does not justify the absence of
              a human helmsman at the controls, observing all  around the horizon and ready to take
              over immediately if needed.

       C.     All vessels must proceed at a safe speed at all times. The factors to be considered in
              determining "safe speed" include but are not limited to:

                     The state of visibility.
                     Traffic density including concentration of fishing or other vessels.
                     Your vessel's maneuverability, with special referenceto stopping distance and
                     turning ability.
                     At night, the presence of background lights such as those from shore, or from
                     the back-scatter of your vessel's own lights.
                     The state of wind, sea and current and  the proximity omavigational hazards
                     The vessel's draft in relation to the available depth of water.

       D.     Meeting Situations

Every vessel must use all available means appropriate including lookout (eyes and ears), radar and
radio to determine if the risk of collision exists. If the vessel is equipped with radar, a radar plot
should be maintained to detect any risk of collision.

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Where possible, contact the other vessel by radio and ask what its intentions are.  Action to avoid a
collision should be taken well in  advance of any potential meeting. Any course of speed change
should be large enough to be obvious to any approaching vessel.  A succession of small alterations
of course should be avoided.

       1.     Meeting Another Vessel Head-On
              When  two power-driven vessels  are approaching one another head-on,  unless
              otherwise agreed each should pass  on the port side of the other. Neither vessel has
              the right of way over the other, and both must alter course to starboard, if necessary,
              to provide sufficient clearance for safe passage.

       2.     Crossing Situations
              When two power-driven vessels are crossing so as to involve risk of collision, the
              vessel on the right has the right-of-way, while the vessel on the left must keep clear.
              Vessels are crossing if they are not meeting head-on or nearly so, and each has the
              other on a relative bearing forward of 22 ]A degrees abaft (behind) the beam, port or
              starboard.

       3.     Overtaking Another Vessel
              If one vessel is coming up on another from more than 22.5 degrees abaft (behind) its
              beam, and making greater speed so as to close the distance between them, the vessel
              astern is said to be overtaking the slower vessel. Any overtaking vessel must  keep out
              of the way of the vessel it is overtaking. The overtaking vessel is burdened, whereas
              the  overtaken vessel  is privileged.  A vessel that is being overtaken shall  keep her
              course  and speed,  but may take action to avoid collision by her maneuver  alone, as
              soon as it becomes apparent to her that the vessel required to keep out of the way is
              not taking appropriate action.

10.0   Mooring (see figure 1)

       A.     The bow line runs through the bullnose or bow chock. The stern line runs through
              after chocks.  These  lines should be run well up the dock to reduce fore and aft
              motion.

       B.     Breast  lines may be run perpendicular from the bow, waist or quarter to  keep the
              vessel from moving away from the  pier.

       C.     Spring  lines may  be run from the bow  or quarter to  eliminate forward or aft
              movement of the vessel.

       D.     Use only spliced eyes in the ends of mooring lines, no knots. Send the eye to  the dock
              so the man on board can tend the line.

       E.     Before you leave the vessel at its mooring, check the condition of dock cleats, piles
              and rails to be sure they're strong enough to hold it.

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       F.      Avoid extreme bends in mooring lines where they pass through hawse holes or
              fairleads (use a minimum bending radius of 3 times the diameter of the line to avoid
              wear or failure).  Use rubber hose or some other material as chafing gear around the
              bends.

       G.     If two bights or eye splices are to be placed over the same bollard, the second should
              be led up and through the eye of the first so that either can be removed independently.

       H.     When making a hitch on a cleat, start with at least one full turn around the base before
              you begin taking turns over the horns.  Use only figure-eight turns, no knots or
              hitches that may jam under tension.

       I.      Synthetic lines are slippery, with little friction; use extra turns.  Watch your hands to
              avoid pinching or crushing injuries.

       J.      When snubbing a line on a cleat,  never try to hold a vessel without first taking turns
              on a cleat.  Stand well back from the cleat, out of the bight and at 90 degrees to the
              angle of pull. Use extra turns with synthetic lines that may slip and pull you into the
              cleat.

11.0   Ground Tackle (see figure 2)

       A.     The "rode" refers to all gear between the vessel and the anchor, including line or
              chain and connecting swivels or shackles.  The rode may be  all chain or a combination
              of chain and wire or line. The rode must be marked by length so the man setting the
              anchor knows how much has been paid out.

       B.     The rode absorbs most of the loading due to waves and should be compliant.  Chain
              and line  best serve this requirement, and if wire rope is chosen for the  rode, a short
              length of chain (10-12 feet) should be used to attach the anchor to the rope.  The
              chain portion of the rode  provides extra holding weight and helps keep the  pull
              horizontal  so the anchor flukes dig into the bottom. Chain withstands the wear of
              lying on the bottom better than wire or line, and helps weigh the anchor.

       C.     The rode should have a breaking strength of five times the anchor hold if made of line
              or wire,  and  two times the design load if made of chain.

       D.     The term "bow roller" refers to rollers that permit the rode to be paid out or heaved
              in, and that serve to secure the anchor when the vessel is underway.

       E.     The term "hawse" refers to pipes or holes that pass through the bulwarks to lead the
              anchor line or chain. The hawse pipes or holes may be used for storing  certain types
              of anchors so that they can be quickly deployed.

       F.      The anchor  must be securely stowed in  it's bow roller or hawse pipe to prevent its
              breaking loose and causing damage in a seaway.

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      G.      Riding chocks, stoppers and dogs are devices that take the strain off the winch or
              windlass when the vessel is riding at anchor.  The anchor rode should always be
              stoppered or dogged so the vessel doesn't ride on the winch or windlass brake.

      H.      A "wildcat" is a sprocket wheel that engages the anchor chain as it is being hauled
              in on vessels equipped with windlasses. The wildcat should always be dogged when
              the vessel is riding on the chain.

      I.      The terms "capstan" or "gypsy head" refer to vertical or horizontal drums fitted to
              a windlass for handling the line portion of the anchor rode or other forward mooring
              lines. When you're working a line on a capstan or gypsy head, stand as far back as
              possible and watch your hands. Where possible, stand at right angles to the direction
              of pull.

      J.      On vessels equipped with windlasses, after passing over the wildcat or gypsy, the
              chain or line drops down the chainpipe into the chain locker below.

      K.      The bitter end of the line or chain must be securely fastened in the chain locker to
              prevent losing the ground tackle overboard.  On vessels equipped with winches and
              wire rods, leave one full layer of turns on the drum to "lock" the wire in place.

      L.      Scope is defined as the length of the rode (measured from the hawse or bow roller)
              compared to the depth.  A scope of 7 to 1 is the general rule for anchoring (7 feet of
              rode for every foot of depth), although this rule may not be practical in deep water.
              Adequate scope is essential to enable the flukes of the anchor to dig into the bottom.
              With adequate scope, every pull of the vessel sets the anchor deeper. TOO little scope
              and the pull of the vessel lifts the shank and the flukes come free. Too much scope
              and the rode may part because of the additional weight of the line, wire or chain itself.

      M.     The term "shots" refers to lengths of chain connected to form the anchor cable. The
              standard shot is about 15 fathoms long. Anchor elements consist of:

                    Flukes or palms (the flat members that grab the bottom).

              "      Shank(the metal bar that joins the crowns to the ring or hole that accepts the
                    rode).

              "      Stock(a crosspiece that helps position the flukes so they dig into the bottom).

12.0  Anchoring (see figure 3)

      A.      Don't leave the anchor windlass in gear when you are underway. Use the hand brake
              and dogs or stoppers in case you lose power and have to set  the anchor manually.

      B.      The anchor should always be let go with the vessel moving slowly to avoid paying the
              chain down on top of the anchor and fouling the flukes. Under normal conditions, let
              go with slight sternway.

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C.     Always send two men forward to work the anchor gear in case one gets hung-up
       Only one man works the gear, however, and he stands to the side and out of the bight.
       The other man stands well clear.

D.     Don't anchor on underwater cables or pipes, or where your vessel will interfere with
       other vessel operations.

E.     When you're letting go, maintain control over the anchor and rode by means of the
       winch or windlass brake. If you are handling the chain over a wildcat, never put your
       fingers into the links, use the palm of the hands.  Stand as far back from the wildcat
       as possible and stay out of the bight.

F.     If you are obliged to let go at high speed, let the rode run until the vessel loses way
       sufficiently to make it safe to snub her.  There is no harm in letting out a considerable
       length of rode, then heaving in to the proper scope. Checking the vessel's speed by
       means of her ground tackle may damage the rode to the extent that it later parts under
       moderate stress.

G.     Always maintain an anchor watch to ensure that the  vessel doesn't drag or break free
       unnoticed. The watch should take visual bearings and ranges  to use as reference
       points.   Setting the radar range ring on  the shoreline, and using fathometer depth
       alarms both serve as good warning mechanisms, but  nothing replaces the human eye.

H.     If a storm approaches, the best course of action is to seek the open sea. If you must
       ride out a storm in an anchorage, use extra scope.   It may be advisable to set a
       second anchor to form a mooring, or to drop  a second anchor underfoot to prevent
       the vessel from surging back and forth across the wind.

I.      Ideally,  anchor gear should be inspected annually, with special attention paid to
       detachable chain links, shackles and swivels.  During annual  maintenance, the anchor
       rode should be laid out on deck  and examined for damage throughout it's length.
       Scale and corrosion should be removed, and detachable links, shackles and swivels
       replaced as necessary.

J.      To distribute wear, chain shots should be rotated. On all-chain  rods, a worn or
       defective shot should be shifted to the bitter end until it can be replaced.

K.     The anchor hinges must be lubricated sufficiently to  keep the flukes from seizing up,
       particularly if the  anchor  is rarely used.  Screw-type shackles used to  connect
       segments of the anchor rode should be moused or otherwise locked to prevent them
       from coming loose when the anchor is on the  bottom.

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                             Basic Guides to Boating Safety






1.      Carry proper equipment - know how to use it!!




2.      Maintain boat and equipment in top condition.




3.      Know and obey the Rules of the Road.




4.      Operate with care, courtesy and common sense.




5.      Always keep your boat under complete control.




6.      Watch posted speeds; slow down in anchorages.




7.      Do not ever overload your boat.




8.      See that lifesaving equipment is accessible.




9.      Check local weather reports before departure.




10.    Inspect hull, engine and all gear frequently.




11.    Keep bilges clean, electrical contacts tight.




12.    Guard rigidly against any fuel system leakage.




13.    Have fire extinguishers instantly available.




14.    Take maximum precautions when taking on fuel.




15.    Be sure to allow adequate scope when anchoring.

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             U. S. EPA GLNPO
SUPPLEMENTAL HEALTH & SAFETY GUIDELINES
                 FOR
GRANTEES, CO-OPERATORS, AND CONTRACTORS


               MARCH 1995

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INTRODUCTION/GLNPO HEALTH & SAFETY POLICY STATEMENT

It is the policy of the U.S. EPA, Great Lakes National Program Office (GLNPO), that all EPA
employees, grantees, co-operators, contractors, and special program participants are entitled to a
comprehensive health and safety program.  The goals of the program are to ensure that each person
working on board GLNPO research vessels is able to work in an environment free of recognized
hazards, and to reduce and mitigate occupational accidents as much as possible. These goals are
accomplished through:

       1.     Training in the proper techniques for field investigations, proper use of and access to
              safety equipment, and proper protocol for inspections;

       2.     Development of standard operating procedures for  routine and non-routine work
              environments;

       3.     Enabling employees and contractors to identify those work situations where they
              believe a hazard exists without  any adverse action taken against them.

This document outlines polices, responsibilities, procedures, and guidelines, pertinent to Grantees,
Co-Operators and  Contractors, as  stated in the GLNPO  Health, Safety, and  Environmental
Compliance Manual.  This document must be used only as supplemental information to increase the
Grantee's, Contractor's and Co-Operator's awareness of the GLNPO policies and procedures, and is
not meant to be all-inclusive.  A copy of the GLNPO manual and appropriate appendices are available
aboard over-night research vessels, and are available from the GLNPO offices.

These policies and procedures are coordinated by the GLNPO Health and Safety Team in conjunction
with the Regional Health and Safety Manager. In addition to the policies and procedures set forth
by GLNPO, all applicable requirements of the  Occupational Safety and Health Administration, the
provisions of the Executive Order 12196 and other relevant statutes, laws, polices and guidance of
the Federal Government regarding  occupational health and safety, as well as environmental
compliance, shall be implemented.
RESPONSIBILITIES

EPA Personnel

The responsibilities of relevant EPA personnel are detailed in Chapter One of the GLNPO Health,
Safety, and Environmental Compliance Manual.

Contractors. Grantees. Co-Operators

All Contractors, Grantees, and Co-operators that are involved in field activities on EPA vessels are
required to have read the contents of this document as well as adhere to the specific requirements as
outlined by GLNPO.

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All Contractors, Grantees, and Co-operators performing work for Region V, or for any EPA activity
or organization serviced by Regional Safety Personnel, shall contain provisions, in writing, which
require these persons to follow, at a minimum, the same rules and requirements that U.S. EPA
personnel follow while performing similar work.  Wherever possible, contractual provisions should
require that  all safety  equipment  and services (e.g.  personal  protective equipment, medical
monitoring, training, etc.) be equivalent to that required for EPA personnel.

Designated Chief Scientists, while undergoing surveillance and research operations, should ensure
that all survey  activities are conducted safely and in  accordance with the health, safety,  and
environmental protocols of GLNPO.

All persons are  expected to follow all health and safety rules and must perform their duties in a
manner which will protect their own well being and that of their fellow workers.  Persons are required
to use safety equipment, personal protective equipment, and other safety devices and, moreover, to
follow safety procedures that GLNPO deems necessary for thsir protection. Refusal or neglect to
follow GLNPO policies and procedures can result in dismissal from EPA vessels.

Reporting of Un-Safe/Un-Healthful Work Conditions

If a persons considers a situation or a  work condition to be un-safe or un-healthful, it is  his/her
responsibility to report it to either the Chief Scientist, Captain, or Ship's Chemical Hygiene and Safety
Officer, as soon as the potential hazard is recognized. The GLNPO Health and Safety Manager, in
conjunction with the Regional Health and Safety Manager, and his/her staff will investigate the
situation and recommend corrective actions, if necessary.
INJURY & ILLNESS

When a persons surfers an injury or illness, whether traumatic or non-traumatic, while on board EPA
vessels, there are necessary procedures which must be followed in order to protect the person's
interest and well being.  These procedures are as follows:

       1.     Inform your supervisor as soon as possible

       2.     Report the injury/incident to the Bridge or Safety Officer

       3.     Complete Accident/Injury/Incident Report

Note:  In the event of a medical emergency involving bodily fluids, it is GLNPO Policy that only
       Designated  First Aid Responders who have received  appropriate First Aid/CPR and
       Bloodborne Pathogen training participate in response activities.

Investigation

In the event of an injury or illness, an investigation will be conducted. Investigation of an accident

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or illness is necessary to prevent similar occurrences.  It is not designed to establish guilt or
innocence, but is an attempt to identify and abate potential hazards.

Record-Keeping

In accordance with OSHA regulations, it is the responsibility of the employer to maintain and keep
current special Injury and Illness forms for  submission to the Department of Labor.   It  is
recommended that all contractors, grantees, and co-operators research their  responsibilities  in
complying with this regulatory requirement.
TRAINING, CERTIFICATION, AND MEDICAL MONITORING

All EPA contractors, grantees, and co-operators who work in EPA owned or leased laboratory space
(including laboratories on research vessels) must meet the safety training requirements specified in
EPA Order 1440 and Regional Safety Manuals.  Supervisors must ensure that their employees are
aware of these requirements and that applicable requirements are implemented.

Chemical Hygiene

GLNPO has developed a Chemical Hygiene Plan in accordance with OSHA 29 CFR 1910.1450,
"Occupational Exposures to Hazardous Chemicals in Laboratories". All laboratory personnel must
receive training based on the contents of the GLNPO Health and Safety Manual and the Chemical
Hygiene Plan.  Additionally, contractors, grantees and co-operators are encouraged to pursue their
own responsibilities in regards to the Standard.

24-Hour Laboratory Safety Course

As specified in Order 1440, Chapter 8, a 24-hour Laboratory Safety Course is required by all EPA
and contractor personnel prior to working in an EPA laboratory.  A training agenda can be found in
Chapter 2 of the GLNPO Health, Safety, and Environmental Compliance Manual, Attachment 2.1.
An annual 4-hour refresher class is also required.

GLNPO Research Vessel Laboratory Training

In addition to the 24-hour Laboratory Safety Course and  4-hour annual refresher, all laboratory
personnel  are required to attend a "site-specific" training orientation course prior to engaging in
laboratory activities on-board EPA research vessels. This training will be performed on board the
vessel prior to departure.

Radiation Safety Training

A site-specific training course is required for all personnel  prior to working in laboratories where
radioactive materials are used.

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Other training not listed in this document may be required based on specific job assignments and
responsibilities (i.e., Fire Fighting, First Aid/CPR, Bloodborne Pathogen, Respiratory Protection,
Emergency Response, Cranes, Hearing Conservation, Hazard Communication, Lockout/Tagout,
etc.). To ensure compliance with GLNPO policies, it is recommended that contractors, grantees, and
co-operators review Chapter 2 of the GLNPO Health, Safety, and Environmental Compliance Manual
for further training requirements.

No persons, whether EPA employees, contractors, grantees, or co-operators will be allowed to
participate in GLNPO sponsored activities until they have received appropriate training.
COMPLIANCE REVIEW AND STANDARD OPERATING PROCEDURES

Any contract which involves field or laboratory work or otherwise appears to impact health, safety,
or the environment must be reviewed and approved by the Safety Manager while in the initial
planning stage.  In accordance with the GLNPO Health, Safety, and Environmental Compliance
Manual, all EPA contracts must contain provisions which require the contract employee to follow all
safety, health, and environmental compliance rule and regulations.

Written Health and Safety Standard Operating Procedures (SOP) must be developed for specific tasks
related to field or laboratory operations.  Health and Safety SOPs may address issues such as Safety
Procedures, Personal Protective Equipment (PPE), Training Requirements, etc.  Additionally, all
marine sampling, recovery, and operational activities are required to have SOPs for specific activities
which impact safety, health, or environmental compliance.
RESEARCH VESSEL HEALTH AND SAFETY

Unique to GLNPO research vessels is their on-board laboratories.  This feature, in addition to normal
marine operations, requires adherence to rigorous health and safety procedures. It is the policy of
GLNPO to ensure that all persons aboard each research vessel receive health and safety training
relative to the specific hazards they may encounter while on board.

Attachments  to this document contain basic health and safety guidelines to be followed while on
board EPA vessels.

In conclusion, one must remember, all the programs, training, and rules in the world can not replace
the need for common sense.  In most situations, common  sense can prevail.  If people just take a
moment to review their task, and identified any potential hazards associated with the task, and take
preventative  measures to minimize or eliminate such hazards, you can greatly reduce  any risk
associated with the task.  Being aware of the hazards in the environment around you and being
prepared can  be the key to performing tasks safely.

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ATTACHMENTS

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               HEALTH & SAFETY GUIDELINES FOR
                     LABORATORY OPERATIONS

Although'the following guidelines highlight some of the primary health and safety issues aboard the R/V
Lake Guardian, by no means do they provide a thorough instruction and training program. All guests are
required to attend the initial Health and Safety Orientation/Video presented at the beginning of the cruise,
as well as review the GLNPO Health. Safety & Environmental Compliance Manual, located in the
laboratory office.

1.     No eating, drinking or smoking permitted in laboratories.

2.     Safety glasses and laboratory coats must be worn at all times when working in
      the laboratories.

3.     No shorts or sandals are to be worn in the laboratories.

4.    i Short sleeves are permitted in the laboratory only  if lab coats are worn.

5.     Laboratory equipment must be secured against  rocking of the ship.
                                  i

6.     The Chemical Hygiene Officer (CHO) must be informed of all  chemicals
      brought onboard.

7.     A Material Safety Data Sheet (MSDS) must accompany every  chemical
      brought onboard the R/V Lake Guardian.

8.     The CHO must be informed of all chemical waste streams.

9.     The CHO must be informed of any chemical spill.

10.    Groups using the ship are responsible for containing and removing any
       hazardous waste they generate.

11.    No waste is to be discharged overboard (except virgin sediment).

12.    Spill kits are located in every laboratory.

13.    No laboratory work  may be conducted in the hazardous materials locker.

14.    The carry-buckets provided in the hazardous materials locker must be used  to
       transport material to and from the locker.

15.    Hazardous material in quantities greater  than 4 liters must be stored in the
       hazardous materials locker.

16.    Please provide your  own secondary supplies, such as  tape, pens, computer
       disks, gloves, safety glasses, etc.

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              HEALTH AND SAFETY GUIDELINES FOR
                            SHIP OPERATIONS

Although'the following guidelines highlight some of the primary health and safety concerns aboard the R/V
Lake Guardian, by no means do they provide a thorough instruction and training program. All guests are
required to attend the initial Health and Safety Orientation/Video presented at the beginning of the cruise,
as well as review the GLNPQ Health. Safety & Environmental Compliance Manual, located in the
laboratory office.

1.     Remember the general safety rule when moving about the ship "One hand for
      you, one hand for the ship" at all times.

2.     Due to machinery, noise levels and other personal hazards, the engine room,
      accessible via the galley, is off limits.

3.     Fire doors must be kept closed at all times.
     i
4.     Exit doors, spill kits, safety showers, eye washes and  fire extinguishers must
      be unobstructed.

5.    The top of the container laboratories may not be used  as an observation area or
       for recreation.

6.     Report any observed or suspected spills from the ship  into the water to the
       Captain and the CHO immediately.

7.     No liquor, drugs or weapons are allowed.

8.     Smoking is allowed on the outside decks where there is no gasoline
       storage.  Absolutely no smoking when we  are taking on fuel.

9.     Access to the bridge is allowed preceded by a knock on the door. Enter only
       from the leeward side (opposite the side from which the wind is blowing).

 10.   When on the bridge please refrain from speaking until spoken to as the Captain
       or mate on watch may be  in the process of receiving/sending radio
       communications or  plotting a course.

 11.   Please interrupt your conversation when you hear traffic on the radio until the
       Captain or mate on watch signals that it is acceptable  to resume conversation.

 12.   When the ship is entering or leaving port,  or during periods of poor visibility
       or high traffic activity, and at the Master's discretion, the pilot house should
       not be visited.  You may assemble on the 0-2 level (boat deck) if you so desire.

 13.   Please keep the  noise level to a minimum and do not let doors slam behind you
       so as not to disturb sleeping shipmates.

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               HEALTH &  SAFETY GUIDELINES FOR
                    DECK/SAMPLING OPERATIONS

Although "the following guidelines highlight some of the primary health and safety issues aboard the R/V
Lake Guardian, by no means do they provide a thorough instruction and training program.  All guests are
required to attend the initial Health and Safety Orientation/Video presented at the beginning of the cruise,
as well as review the GLNPO Health. Safety & Environmental Compliance Manual, located in the
laboratory office.
1.     Work vests must be worn while working on the fantail and rosette deck.

2.     Sandals and other open-toed shoes are prohibited when working on deck or in
      the laboratories.

3.     Hardhats, gloves and steel-toed shoes must be worn when working in conditions
      where there is a possibility  of injury to the head, hands or feet. Common sense
      and good judgement should dictate the appropriate safety gear to be worn in
      any given situation; however, if in doubt, please ask the Chemical Hygiene
      Officer.

4.     No one is to work alone on the fantail at any time.

5.     The fantail is not  to be used as an area for recreation.

6.     Persons on the fantail while the crane or A-frame is in operation must wear a
      hard hat.

7.     Only authorized ship's crew are allowed on the fantail during docking and
      undocking procedures. Do not attempt to access the fantail during these
      procedures.  Absolutely no one is allowed to depart the vessel until the  "All
      Clear" is announced by the Captain.

8.     Only authorized ship's crew should operate the ship's equipment such as
      winches, davits, A-frames and cranes; however, during extended operations
      scientific personnel may be asked to participate in these evolutions.

9.    While steaming during hours of darkness, no unauthorized lights should be
      displayed on deck.  This is to comply with the  "Rules of the Road"; lights
      shown forward of the pilot  house are detrimental to the night vision of the
      conning officer.  While on  station and engaged in sampling/testing operations,
      adequate aft-facing night lighting will be provided.

10.   Scientific personnel should  direct all inquiries to the Master through the Chief
      Scientist in matters concerning work on deck, navigation, safe working
      parameters, use of ship's equipment, etc.  Use of this procedure will avoid
      working at cross-purposes.

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                      HEALTH & SAFETY GUIDELINES
             EMERGENCY OPERATIONS AND PROCEDURES

Although the following guidelines highlight some of the primary health and safety issues aboard the R/V Lake
Guardian, by no means do they provide a thorough instruction and training program. All guests are required to
attend the initial Health and Safety Orientation/Video presented at the beginning of the cruise, as well as review the
GLNPO Health. Safety & Environmental Compliance Manual, located in the laboratory office.

1.      First concern is the safety of yourself, the crew and the ship.

2.      Anyone taking personal medication should so indicate on the "In case of
       Accident" form.  All guest  scientists are required to fill out this emergency
       medical form during  the safety orientation.

3.      All injuries or illnesses are  to be reported to the bridge as soon as possible so
       that proper attention  may be given to the patient.

4.   /In the case of emergency, you can reach  the pilot house from any phone on the
       ship by simply  dialing 911.

5.     First-aid kits are located in all of the laboratories and in the Captain's office.

6.     Safety equipment found in your stateroom:
              a. Personal Flotation Device #1 -  Life Preserver
              b. Personal Flotation Device #2 - Immersion Suit
              c. Kick-out panel in door.

7.     In the event of a  fire or abandon-ship emergency, take both of your PFD's
       mentioned above  to your assigned life boat on the 02 deck (i.e. boat deck or bridge
       deck) and await further instructions. Do NOT don your life preserver or immersion
       suit inside of the  ship.

 8.     In the event of a  man-overboard emergency:
              a. Yell, "Man overboard!"
              b. Throw  life ring or any  item that will float.
              c. Keep the person in sight.
              d. Notify  the Bridge as soon as possible (dial 911).

 9.     Refer to a station bill for your life boat assignment and explanation of alarm
       signals.  Station bills are located  in the galley, Captain's office, passageway outside
       of the biology  lab and  in the pilot house.

 10.   A test of the ship's general alarm is performed daily at noon.

 11.   The ship's crew is trained and certified in CPR and Basic First-Aid Response.

 12.    It is the responsiblity of the visting party's supervisor to ensure that all guest's under
        his/her direction  are accounted for in case's of rough  seas.  Should members of the
        party become severly seasick, careful monitoring of their condition would be
        requested to avoid extreme physical or even mental deterioration such that their
        personal health and  safety would be in jeopardy.

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                               R/V LAKE GUARDIAN
STANDARD OPERATING PROCEDURE - PREPARATIONS FOR EMERGENCIES
PURPOSE

This Standard Operating Procedure (SOP) has been developed in accordance with 46 CFR 185.25,
for the  preparation  of emergencies.  It is the duty of the Master to ensure that this SOP is
comprehensive and that the "Emergency Checkoff List" contained in this document is posted in a
conspicuous place (ie. the Pilot House, and/or Lounge) accessible to all Crew Members and
Passengers.

POLICY

In accordance with 46 CFR 185.25 and GLNPO Policy, before getting underway the Master of the
vessel shall ensure that suitable public announcements, instructive placards, or both are provided
in a manner which affords all passengers the opportunity to become acquainted with:

       1.     Stowage location of life preservers;
      2.     Proper methods of donning and adjusting life preservers of the type(s) carried on
             board the vessel;
      3.     The type and location of all life saving devices carried on board the vessel; and
      4.     The location and content of the "Emergency  Checkoff List" contained  in this
             document

EMERGENCY CHECKOFF LIST

In accordance with 46 CFR 185.25-5, an "Emergency Checkoff List" has been developed for the
R/V Lake Guardian and appears as Attachment 1 of this  document.  It is the duty of the Master to
ensure the list is comprehensive and is posted in a conspicuous place where it is  accessible for
viewing by all persons on board the vessel.

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ATTACHMENT 1:  EMERGENCY CHECKOFF LIST


Rough Weather

Rough weather, at sea or crossing hazardous bars:

       1.      Close all water-tight and weather tight doors,  hatches, and portholes to prevent
              taking water aboard.

       2.      Keep bilges dry to prevent loss of stability due to water in bilges.

       3.      Keep passengers stationary and off weather decks.

       4.      Have passengers put on life preservers if the going becomes very rough or you
              are about to cross hazardous bar.

       5.      Never abandon a vessel unless actually forced to do so.

       6.      If assistance is needed use the International Distress call over the  radiotelephone
              or call the USCG immediately.

       7.      Prepare life rafts for launching.


Man Overboard

       1      Hail  "Man Overboard"

       2.      Throw a ring buoy overboard as close to the person as possible.

       3.      Mark location using differential GPS

       4.      Post  a lookout to keep the person overboard in sight.

       5.      Maneuver the vessel to pick up the person in the water.

       6      Have crew member put on life preserver, attach a safety line to him and havehim/her
              stand by to jump into the water to assist the person overboard if necessary.

       7      If person is not immediately  located, notify the  USCG and other vessels in the
              vicinity by radiotelephone.

       8      Continue search until released by USCG                                              ™

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Fire At Sea

       1.      Cut off air supply to fire, close hatches, ports, doors, and ventilators, etc.

       2      Immediately use  portable  fire extinguishers at base of flames for inflammable
              liquid or grease fires or water for fires in ordinary combustible materials.

       3.      If fire is in machinery space, shut off fuel supply and ventilation,  and discharge
              fixed C02 extinguishing system.

       4.      Maneuver vessel to minimize effect of wind on fire.

       5.      If unable to control fire,  immediately notify USCG  and other vessels in the
              vicinity  by radiotelephone, etc.

       6      Move persons away from  fire, have them don life  preservers, and if necessary,
              prepare  to abandon ship.

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                                                                    REVISED 5/31/97

                 IN-PORT VISITOR FIRE AND EVACUATION PLAN

This In-Port Visitor Evacuation Plan will be in effect in all ports of call, including Bay City, Michigan,
during all prearranged group guided tours and all public open houses. Establishing communication
links with appropriate emergency and port  authorities will be done prior to, or immediately after,
entering all poi :s of call.

When entering or upon arriving at a planned port destination, the Master or Mate on watch will
establish a means of contacting the local Fire Department, Police and emergency medical response
service while in port.  This information will be made visibly prominent  on the Bridge and on the
Quarter Deck. Every effort shall be made by the Master or Mate on watch to arrange for the local
Fire Department, Police, and EMS as may be practicable, to visit and tour the R/V Lake Guardian.
Additionally, local emergency response services shall be provided with a copy  of this evacuation plan.

Prior to the beginning of on board tours, the Master or Senior Ships Officer on board, in concert with
the USEPA Public Affairs Representative, will designate an area off ship to reassemble the  on board
tour group in the event of an evacuation. The purpose for the reassembly area is for establishing
accountability of the visitors.  Prior to tour  activities and visitor arrival, the Master or Senior Ship
Officer on board will brief the Tour Guides and Deck Watch with the location of the off ship
reassembly area and the immediate areas off ship to be kept clear in the event of incoming emergency
vehicles.

It is the NUMBER ONE PRIORITY of all shipboard crew members, whether on or off duty, to
facilitate the evacuation of tour groups or open house guests in the event of a fire or ship emergency
events. Should the Fire Alarm or General Alarm be sounded during the hours of public visitation, all
crew members will make a conscientious effort to locate and help evacuate any visitors encountered
on their way to their assigned emergency station.

All  Tour Guides, Deck Watch Members,  and ship personnel on watch during public admittance days
are required to be in direct radio contact with the bridge, Marine Channel 73, at all times.  In  addition,
all personnel must carry dependable flashlights for use in the event of power failure.

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TOUR GUIDES.
 PREPLANNED GROUP TOURS

During all preplanned tours, the Tour Guide or Senior USEPA Public
Affairs Representative shall "log" the names of each individual in the
groups being brought on board.  The log is to remain at the reception
desk located on the Quarter Deck.  The Tour Guide will inform the
group of evacuation procedures including the following:

1.     Point out the off ship reassembly area and explain the necessity
      to determine the accountability of guests

2.     Request that all visitors remain together as much as possible
      during the tour

3.     Inform guests that physically handicapped individuals must be
      accompanied by an able body adult capable of carrying the
      individual off the ship in the event of an emergency situation
QUARTER WATCH:
Throughout the entire open house period, the Quarter Deck Watch
shall diligently maintain a body count of all visitors coming on board
and all visitors exiting the ship.  This count is essential to ensure that
the number of visitors on board is limited in accordance with USEPA
directives.  Most importantly, the count is necessary to ensure that
all open house visitors on board can be accounted for at any given
moment should evacuation of the vessel become necessary.
               WHEN FIRE ALARM OR GENERAL ALARM SOUNDS
CREW MEMBERS:
BRIDGE:
TOUR GUIDES:
Follow the ship emergency billet assignments and ship protocols with
the exception of the tour guides and the assigned Quarter Deck Watch
until all visitors are safely off the ship and the Bridge directs you to
proceed to your assigned billet. Evacuation of visitors is the NUMBER
ONE PRIORITY for all ship crew members whether on or off duty.

Notify the Fire Department or  appropriate shoreside  emergency
response group.

Calmly lead your tour group along the best ship evacuation route to
the shore reassembly area.

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BRIDGE
QUARTER WATCH:
TOUR GUIDES:
As soon as information is available, announce over the ship's PA system
the location of the fire or emergency situation. Advise Tour Guides
and Quarter Deck Watch of development via radio. Make announce-
ments approximately every 30 seconds over the ship's PA system, with
clear, concise directions, for all visitors to  evacuate the ship and the
locations to avoid.  This will assist all crew members in providing
assistance and direction.

Maintain your station to assist all visitors ashore until the Bridge
advises you that the  evacuation is complete.  Take special care to
make an accurate head count of departing visitors.  When the head
count equals the number counted on board, notify the bridge.

Notify the Bridge as soon as you reassemble  your  tour  in the
designated  area off ship and have accounted for all visitors.
CREW MEMBERS.
BRIDGE.
While responding to your assigned billet station, a concerted effort shall
be made to look for and seek out any shipboard visitors.  If you
encounter a visitor, assist them off ship or provide directions using the
safest  route. If encountering a  tour group, ask the  tour guide
if they need any assistance in guiding the people ashore. If assistance
is needed, do so before reporting to your station. When your area of
the ship is clear of visitors and you are on station,  communicate this
fact to the Bridge.

When the Fire Department or other emergency response units arrive,
advise all crew members of further actions to take.  The Master or
Senior Ship's Officer on board will interface  with   the  emergency
response unit.

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SURVEY PLAN
                          Page 1 of 8
                                       Date:
This form is provided to the user/sponsor for assistance in determining the vessel requirements for the project  Close attention to detail i:
essential to the success of the project. So please, please complete the form as accurately as possible.  Draw a single line through any item thai
does not apply. Do not include any information which is or may be deemed classified Additional information may be provided on the last page
1.0 GENERAL
Project Title:	
Organization:   	
Project/Work Assignment Manager:
Survey Chief Scientist:	
             _Survey Title:,
                               .GUARDIAN Requested by:.
                                Organization..
                 .Organization:.
                                 .Organization Address:
Phone No. •
Fax No :.
Principal Investigator:.
FAX No.:	
Comments.
 EPA Grant/Contact:.
	Organization:
Work Assignment No.:_
                                                         _P.I. Telephone No. •_
2.0 SCHEDULE OF OPERATIONS
       FUNCTION    DATE  TIME  LOCATION
Commence Mobilization   	   	   	
Pre-sail Conference       		
Depart Home Base       	   	  	
Depart Station           		
Start Demobilization      	   	   	
 Post-sail Conference     	   	   	
Allowable Weather/Breakdown Days
Comments:      	
                                FUNCTION
                                Complete Mobilization
                                Dock Trials
                                Arrive on Station
                                Arrive Home Base
                                Finish Demobilization

                                Maximum Duration (Days)
                                     DATE  TIME  LOCATION
3.0 CHEMICAL BACKGROUND INFORMATION  (Including standards, spikes, and instrument calibration chemicals)
NOTICE: A \fATERIAL &4FETYDATA SHEET (MSDS) MUST ACCOMPANY EVERY CHEMICAL OR REAGENT THAT IS BROUGHT
ABOARD THE LAKE GUARDIAN. COMPLIANCE WITH THIS REQUEST IS ABSOLUTELY NECESSARY PRIOR TO THE SHIP'S
DEPARTURE. ADDITIONALLY, CHEMICAL WASTES GENERATED BY SHIP'S USERS MUST BE REMOVED BY USERS. THE USER
IS RESPONSIBLE FOR PRODDING PROVISIONS FOR PROPER WASTE STORAGE METHODS/CONTAINERS     CHEMICAL
NAME
      QUANTITY
                     CONCENTRATION

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SURVEY PLAN               Page 2 <**                    Date:
4.0 SURVEY JUSTIFICATION AND RATIONALE
5.0 OBJECTIVES
Project:  	
Survey:
6.0 ENVIRONMENTAL MANAGEMENT QUESTIONS ASKED BY PROJECT/SURVEY
7.0 SURVEY LOCATION AND DESCRIPTION (At*c
Survey Area(s) Name(s) or Geographic
Name:
Survey Area Locations (Provide MaP>:
Survey Area Boundary Coordinates (Lat/Long):
Survey Station Types (Water/s«taent):
Number of Stations By Type'
Water Depth Required:    	 Sediment Zone Required:
Survey Transect Lengths'  	 Transect Line Spacings'
Other 	

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SURVE
Page 3 of 8                 Date:
8.0 SURVEY/SAMPLING METHODOLOGIES
Method Descriptions:
Method Rationale:
Diving On Survey:      YES:  	         NO:   	   (ify«,«*section 15 and 19

9.0 SEQUENCE OF SURVEY TASKS/EVENTS
10.0 SURVEY LOCATION AND DESCRIPTION
1. HF                          	            FREQ
2 VHP                         	            FREQ
3. UHF                         	            FREQ
4 LORAN-C                     	
5. RADAR                      	
6 GYROCOMPASS                	
7. SATNAV                     	
8 OMEGA                      	
9. DECCA                      	
10 OTHER COMMUNICATION       	
1 1 OTHER NAVIGATION               	

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SURVEY PLAN               Pagers                  D.te!

11.0 SfflPBOARD SERVICES EQUIPMENT SUPPLIES
  1.   HANDLING EQUIPMENT
      PROJECT SUPPLIED	 GUARDIAN SUPPLIED (Attach GUARDIAN check L* ifAPProPnate):
  2.   LABORATORIES REQUIRED
      PROJECT SUPPLIED	 GUARDIAN SUPPLIED (Attach GUARDIAN che
  3.   DIRECT DECK ACCESS
  4.   WINCH REQUIREMENTS
      PROJECT SUPPLIED 	 GUARDIAN SUPPLIED (Att,ch GUARDIAN ch«<*u« if APProPnat.):.
  5.   LIFTING GEAR
      PROJECT SUPPLED 	 GUARDIAN SUPPLIED (AH** GUARDIAN check
  6.   OTHER DECK EQUIPMENT:
  7.   ELECTRICAL POWER REQUIREMENTS
             Description and Location: 	
  8.    WATER REQUIREMENTS
             Fresh         Quality       Distilled             Quantity
  9.    HYDRAULICS
             Pressure      Volume
  10   AIR
             Pressure      Volume
  11.   SMALL CRAFT
       PROJECT SUPPLIED	 GUARDIAN SUPPLIED (Attach GUARDIAN check u«ifAppropna«):
       IF PROJECT SUPPLIED, PROVIDE
       Size	       Weight	    O.B. Motor	
       Purpose	
       Will the Guardian need to carry the small craft?     YES	     NO.

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 SURVEY PLAN                P.*S«*                    D.te
fll.O SHIPBOARD SERVICES EQUIPMENT SUPPLIES (Continued)

   1 2.   CREW ASSISTANCE - The crew of the Lake Guardian includes a Science Officer and Marine Technician who are     responsible
 for operating the ship's science, sampling and laboratory equipment to facilitate your survey goals.  There are  alsoseveral professional seamen
 on board to handle the deck gear (cranes, winches, A-frames, davits, etc...) for your use.     Beyond this scope however, it is requested that
 your scientific party include the appropriate number of personnel to complete      the remainder of your project requirements

               Rating (Type)   _        Number of Personnel    _

               Rating (Type)   _        Number of Personnel    _
               Rating (Type)   _        Number of Personnel    _
   1 3.   OTHER SHIP REQUIREMENTS:
        1. _
        2. _ __ _ ________
   14.   SHORE SUPPORT MOBILIZATION FORCE:

               Welders        	        Riggers        	

               Mechanics      	        Machinists     	

               Laborers       	

 12.0 OPERATING

 Sea State:      Wave Height     MAX   	   MIN          	
 Atmospheric Conditions:
               Wind Speed     MAX   	   MIN          	  Direction
               Temperature     MAX   	   MIN          	
               Cloud Cover     ANY   	   O'CAST        	  SUN
               Precipitation Permitted    	   Not Permitted   	
               Visibility        MAX   	   MIN          	
               Time of Day     ANY   	   DAYLIGHT    	  NIGHT

 13.0 SCIENTIFIC PARTY

 1. Point of Contact	
 2. Number of Personnel	       Maximum Number of Persons to a Cabin	
  NAME              SURVEY                     ORGANIZATION            TIME/PLACE OF
                      RESPONSIBILITY                                        ARRIVAL AND DEPARTURE
 1).
 2)_
 3).
  _
,10).

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SURVEY PLAN
                       Page 6 of 8
                                                                          Date:
Please indicate below if any persons in your science party have taken any courses or obtained training and/or certification in
       A.     Laboratory Health & Safety        B
       C.     Hazardous Materials Handling      D.
       E.     Respiratory Protection            F.
       G.     First Aid and/or CPR             H.
       I.      Small Boat Handling             J
       K     Crane/Derrick Operation          L.
                             Field Health & Safety
                             Emergency Spill Response
                             Radiation Safety
                             Fire Fighting
                             Lockout/Tagout
                             Bloodbome Pathogens
 NAME


1)	

2)	

3)	
TRAINING, COURSE OR CERTIFICATION OBTAINED
OBTAINED WHEN
COMMENTS:
14.0 PROPOSED REPORTING REQUIREMENTS

   12.  CREW ASSISTANCE

       Debriefing Telephone Call-       YES   	     NO

       Survey Report Due Date no o,y, Aft« Demoby	
                                                  No. Of Days After Demob.
       Final Report/Other Document Description.

       Other Comments'	
15.0 DIVING OPERATING
Locations:
Potential Hazards:

Depth Range.
                      Maximum Depth'

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SURVEY PLAN
Page 7 of 8                   Date:
Dive Master.    	        Organization:
Dive #2:       	        Organization:
Dive #3:       	        Organization:
Dive #4:       	        Organization'
Dive #5:       	        Organization:
Dive #6:       	        Organization:
Dive #7:       	        Organization'
Dive #8:       	        Organization:
Dives' Tasks:    	
REQUIREMENTS REMINDER:
                 OXYGEN WILL BE ON SITE ON BOTH THE GUARDIAN AND TENDER BOAT.
                                (Oxygen Will Be Provided By The GUARDIAN.)
               STANDBY DIVER WILL BE SUITED UP IN THE TENDER BOAT READY TO DIVE.
                       (Dive Team Scheduling And Bottom Time Planning Must Allow For This)
i GUARDIAN Anchored:  YES: 	       NO: 	              Tender Boat Anchored:    YES: _      NO:
Communications'	
Tender Boat.	
Special Equipment Needed:	

Other:	

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SURVEY  PLAN
                                                                       D.te
16.0 DIVER EMERGENCY AID LIST
DIVERS ALERT NETWORK (DAN)
                                                 24-Hour Telephone No.:
                                                                                   (919)684-8111
NEAREST DECOMPRESSION CHAMBER 0*0%
Telephone No.:
                                                 24-Hour Telephone No
Telephone Call On Day Of Initial Diving Operations To Be Made By:	
Hyperbaric Physician(s):	                Telephone No...
NEAREST HOSPITAL (Facility Name And Addrew):
Telephone No. :
Hyperbaric Physician(s)-
                                                 24-Hour Telephone No
                                                 Telephone No.: _
NEAREST COAST GUARD STATION
VHP Channel.
                                                Telephone No.:
FIRST AID EQUIPMENT:
Location:	
Oxygen Location:.
DIVER MEDIC (Name If Planned)'
SPECIAL CONSIDERATIONS:

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                                                     SECTION: APPENDIX P
                                                     VERSION: FINAL/MAY 1997
                                                     PAGE: COVER
APPENDIX P:  WELCOME ABOARD AND SAFETY ORIENTATION

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     WELCOME ABOARD THE R/V LAKE GUARDIAN
The United States Environmental Protection Agency, Seaward Services, Inc. and the Master, officers
and crew of the R/V Lake Guardian welcome you aboard.  We hope your stay on board will be a
pleasant one. Our goal will be to assist you to bring your research programs to a safe and successful
conclusion.

We appreciate your cooperation in accomplishing this goal and we ask that you review the material
in this handout.  Should you have any questions or encounter problems regarding the items presented
herein, please bring them to the attention of the ship's Master via the Chief Scientist.

This informational handout will provide some essential facts and useful information to ensure you
have a safe and comfortable voyage.  Please do not hesitate to ask questions concerning any subject
about the ship.  We will be happy to provide explanations and/or demonstrations when possible. If
you find you are having a problem, please bring it to the attention of the ship's Master (Captain), the
Mate on watch or the Chief Scientist.

OPERATING AUTHORITY

The R/V Lake Guardian is operated for and under the control of the USEPA's Great Lakes  National
Program Office.  An operating  crew of professional seamen is furnished by Seaward Services, Inc.
Lab support is provided by personnel from Enviroscience. Lab and ship safety overview is provided
by USEPA appointed Chemical Hygiene Officer. When the ship is at sea, a senior scientist from
USEPA/GLNPO is assigned responsibility for all scientific operations and activities aboard  the ship,
in conjunction with the ship's Master.  For all ship operations and safety at sea, the Master has full
legal authority and thus has final control of all ship movements.

MISSION

The R/V Lake Guardian's mission is  to gather  information about the chemical and biological
conditions of the Lakes, and to monitor the pollutant concentrations in water, sediments, air, fish and
other biological matter.  On occasion, the ship may be provided for use for other scientific interests,
including research activities of other federal, state and local agencies as well as academia.

GENERAL DESCRIPTION

The R/V Lake Guardian is USEPA's newest, largest and cleanest surveillance and monitoring vessel.
It was converted in 1990 from a former offshore oil field service vessel  originally built in 1981.
During the conversion period, all  of the structures aft of the exhaust stacks were added. In 1991, the
four container labs and the  HVAC (heating, ventilation and air conditioning) van were added to the
ship, as well as the large stern A-Frame.  The ship is 180 feet in overall length, with a beam (width)
of 40 feet. Depending on loading conditions, it can have a draft (underwater portion of hull) of up

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to 11 feet. Lake Guardian is powered by two 1125 HP Caterpillar diesel engines, each driving its
own propeller shaft.  Electrical power is provided by three 135 KW Caterpillar diesel engines. A bow
thruster, powered by a 300 HP Caterpillar diesel engine, aids in the ship's ability to maneuver in close
quarters. It is also used in station keeping.  When operating at full power, the ship can make 13 miles
per hour and may consume in excess of 100 gallons of fuel per hour.

LABORATORIES AND SCIENTIFIC EQUIPMENT

There are three fLed laboratories located aboard the R/V Lake Guardian:  chemistry lab (main deck
-port side), microbiology lab (main deck  - starboard side), wet lab (main deck-port side aft part of
deckhouse).  In addition, there are four  portable (container) labs mounted on deck which  are
accessed from the passageway connected to the wet lab.  These labs, from forward to aft, are  the
organic extraction lab, organic analytical lab, primary productivity lab, and the hazardous materials
storage locker.

Located on top  of the organic extraction lab is the HVAC van. (This unit contains machinery to
support the analytical and extraction HVAC  systems and no science personnel user equipment is
contained  inside. Science personnel are  requested not to enter this space).  The equipment and
instruments contained in these labs are primarily for support of the basic monitoring function of the
ship. Some or all of this equipment may be made available for use by guest scientists at the discretion
of the USEPA Project Officer, Chief Scientist or his designated  representative.

RESPONSIBILITY OF THE MASTER

The ship's Master (Captain) assumes responsibility for the safe operation and navigation of the ship.
He is responsible for the safety of all personnel on board and may curtail, delay or cease all operations
if, in his judgement, the vessel, personnel or equipment are in jeopardy.  The Master will confer with
the Chief Scientist in making decisions at these times, and will comply with the test plan so far as
possible to achieve the goals set forth therein.

STANDARD OPERATING PROCEDURES

A manual  of Standard Operating Procedures (SOP's) is located on the main  deck passageway.
Included in this manual are procedures for performing certain functions aboard the vessel which could
endanger the safety of personnel or equipment. At their earliest opportunity, all scientific personnel
should read and  become  familiar with these procedures.  Further, in the laboratory office is the
USEPA GLNPO Health. Safety and Environmental Compliance Manual. Again, it is incumbent
upon all scientific personnel to become familiar with this manual and to abide by the requirements
contained therein.

LIFE ABOARD THE R/V LAKE GUARDIAN

Knowledge of the ship's layout will assist you in accomplishing your daily activities, and is vital in
determining the appropriate response for emergency situations, both real and simulated. Please study

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the deck plans, note the locations of fixed fire stations, life rings, portable fire extinguishers and
abandon ship stations.

Because of the R/V Lake Guardian's unique mission, schedules and routines are inherently rigid.  It
is suggested that you keep abreast of vessel movements, scientific activities and the weather so that
you are aware of emerging conditions which may affect you and/or your work aboard the ship.

The R/V Lake Guardian has berthing for up to 43 persons, including the crew.  Most staterooms set
aside for scientific personnel have two fixed bunks (upper and lower) as well as a fold down pullman
bunk for a third person. These pullman bunks are used only when a maximum complement  of
personnel are carried aboard. When this situation arises, some scientists and crew members may have
to share accommodations. Stateroom assignments are made jointly by the ship's Master and the Chief
Scientist.

Each stateroom and bunk contains a card which designates the emergency mustering location for the
persons in that stateroom. Located on the back of each stateroom door is a diagram showing the
occupants of that room the quickest means of egress. You should become familiar with this diagram
soon after being assigned your stateroom.

During the course of your cruise, you will be asked to participate in emergency preparedness drills
in concert  with the ship's operating crew and USEPA personnel.  Emergency drills are a most
important and very necessary part of shipboard life, since they serve to ensure that you and your
shipmates are properly trained in the event of an emergency or casualty to an individual or to the ship.
You will be instructed on how to abandon ship, how to "muster" in one location should a fire occur
(in order to account for everyone), how to report a man overboard, how to operate a hand-portable
fire extinguisher, how to deploy a life ring, how to don a survival suit and life jacket.

Drills can be perceived as a nuisance to some, since at times some individuals will be inconvenienced
by having to participate in drills during their off-duty hours. Although some drills are unannounced,
we try to schedule them within a five hour period between 1000 and 1500, and/or while the ship is
not engaged in sampling operations.

PRE-BOARDTNG PREPARATIONS

Science personnel should allow sufficient time prior to the departure date to turn in rental cars, load
equipment, prepare their staff, check supplies, etc..  Use your enclosed Pre-Boarding Check Off-
Sheet to ensure that you have adhered to the minimum standard requirements and guidelines. Sailing
time may be delayed at the Masters discretion, if science/technical personnel report aboard in a
condition which he considers unsafe. Further delays in sailing are announced should equipment
malfunction occur, or when late deliveries of equipment and supplies are experienced. Current or
forecast weather conditions may also delay sailing time.

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REPORTING ABOARD

Plan to report aboard at least one hour before sailing time.  Upon arrival you should report to the
First Mate, who will sign you aboard and provide an orientation of the vessel's physical arrangements
including assigned berthing area.  In addition, please supply the Chemical Hygiene Officer with your
chemical inventory and provide all necessary Material Data Safety Sheets (MSDS's). For your safety,
you are required to accomplish the following immediately after receiving your stateroom assignment.
       • Ensure that your life jacket is in place near your bunk.
       • Ensure that your survival suit is in place near your bunk.

Shortly after reporting aboard for your cruise, you will either be given a safety orientation by the
Captain of the ship or the Chemical Hygiene Officer, or you will attend the presentation of a safety
video  produced exclusively for the R/V Lake Guardian.  It is imperative that you attend this
orientation  or video when scheduled,  and pay close attention to what the Captain has to say -
someday your life or that of your shipmates may depend on it!

Refer to a nearby station bill and find your assigned life raft as well as your fire and abandon ship
stations.  Station bills are located at several  areas throughout the ship.  These areas include the
Captain's office, focsle deck, mess deck, passageway outside of the biology and chemistry labs and
the pilot house.

Become familiar with the signals for fire and abandon ship (you will hear these in the safety video);
a drill will be conducted upon departure from  the first port and at least once per week thereafter.

       Safety Rule:  One hand for you, and one hand for the ship. This means that when carrying
       large or bulky items, you should always have one hand available and ready to grab a safe
       handhold in case the vessel suddenly rolls, or if you lose your balance.  This may mean making
       several trips with your gear or asking a shipmate to help you. Similarly, keep safe handholds
       in mind so you can grab for them automatically if you must clutch for support.

WORK DECK

The Chief Scientist or his designated representative will advise the Master of any sampling, launching,
streaming or retrieval activity required. The  ship's cranes, winches, tools, rigging, etc. are to be
operated by ship's crew only; however during extended operations scientific personnel may be asked
to participate in  these evolutions.  Scientific personnel should direct all inquiries to the Master
through the Chief Scientist in matters concerning work on deck, navigation, safe working parameters,
use of ship's equipment, etc.  Use of this procedure will avoid working at cross-purposes.

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BERTHING ARRANGEMENTS

Scientific personnel quarters are located as follows:

       Focsle Deck - Five staterooms port and starboard aft of the fire door.
       Main Deck - One stateroom starboard side between the forward fire doors.

Berthing assignments for these spaces are made by the First Mate or Chief Scientist. Linens, towels,
etc. will be provided when you arrive on board.  Upon departure from the vessel, kindly strip your
bunk and store the soiled linen in the laundry basket provided near the washing machine on the port
side of the main deck, forward. Fresh linen will be issued once per week, the time of which will be
announced in advance.

MESSING

Our cook and steward prepare and serve varied and nutritious meals for our crew and the embarked
scientific party.  Normal meal hours are as follows:

       Breakfast: 0730-0830 Lunch: 1130-1230    Dinner: 1700-1800

When large numbers of personnel are carried aboard, it is requested that meals be eaten comfortably
but quickly. Eat and enjoy your meals but please do not "dally in the galley".  We have seating for
19 but on occasion must feed up to 43  personnel.  Located adjacent to the coffee pot is a rack on
which to stow your personal coffee mug.  You are responsible for washing your own mug after each
use.

We request that the mess area be vacated by all personnel 30 minutes before and after each meal in
order that the steward have ample space to prepare the area for meals and to  square away after meals.
Certain other amenities are expected and required:

       1.     Appropriate clothing shall be worn to meals; this includes shirt and shoes.

      2.     Traditional shipboard protocol dictates that hats, caps and other headgear be removed
             when entering the mess area while the ship's company is at mess.

      3.     Some  late arrivals to meals due to watch standing can be  expected; however,
             general nonconformity with the  meal  schedule is  considered poor form.  Ship's
             crew reporting  for watch on occasion may be served ahead of someone who
             has  been  waiting.   Since the crew must report on time, your consideration of
             this possibility will be appreciated.

      4.     Anyone on a special  diet or who has  a need  to eat late, should notify the cook
             and you will be accommodated.

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       5.     When entering the mess area for meals, neither space nor sanitary considerations
              permit visits to the galley to look things over. A menu is posted on the whiteboard.
              Take your dinnerware from the dispensers provided and proceed to the salad bar and
              hot table and then the seat of your choice.

       6.     At all times, the mess area is designated a no-smoking area, as are all interior spaces
              aboard the ship.

       7.     No food is permitted in the lounge during mealtimes; however, snacks are
              permitted in the lounge between meals and in the evening.

       8.     Night rations are available and located in the small refrigerator below the salad
              bar which is designated for your use during  other than meal hours.  Feel free
              to partake of the cold cuts, leftovers, fruit and  soft drinks provided.   Please
              cover, label  and  date any food items returned to the refrigerator.  In addition,
              do not store any food items in the steel, tin or aluminum cans from which they
              were obtained.

       9.     Except for your personal  coffee mug, do  not hand wash dishes or cups,  but
              leave them in the cart provided.   They will  be  properly cleaned and sanitized
              in the dishwasher.

The stainless steel refrigerator in the galley and the walk-in refrigerator and freezer contain food for
preparation of regular meals. We request that you do not open these units, but rather ask the cook
for any item which you cannot find in the salad bar refrigerator.  We further request that any mess
gear used outside of normal meal hours be scraped clean by the user and placed in the cart provided
for dishes and utensils to be cleaned.

WASHING MACHINES AND DRYERS

Washing machines and dryers are provided and available for the use of all personnel aboard.  The
washing machines should only be used when you have a full load; use for partial loads will deplete
the snip's fresh water supply and holding capacity.  Use less than Vz cup of detergent for a full load;
additional soap creates heavy sudsing which strains the  machine and can leave residue in your
clothing. Low phosphate detergent is provided for your use by the ship; do not use other types of
soap in the washing machine. The washing machines may be used while the ship is on station and
collecting samples since the ship has the ability to contain all waste water.

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Personal use of the washers and dryers is permitted within the following format:

       a.     Write name on space provided with attached erasable pen.

       b.     Remember that all washing machine water drains into our sanitary holding
              tank thus reducing our time at sea.   Therefore, in order to conserve water and
              sanitary  tank  capacity,  we ask that  you bring enough clean  clothing such that
              you do not need to continually wash your clothes.

       c.     Do not overload or underload these machines.

       d.     Keep track of finish times and promptly remove clothing from machines to
              allow others to use them.

       e.     Lastly, make sure machines are empty and that lint screens are clean.

       f.      Again, prior to final departure, please  strip your bunk and place all your dirty
              linens inside of your pillowcase and deposit in the laundry room.

POTABLE WATER AND SEWAGE CAPACITY

The potable water and sewage holding capacity onboard the R/V Lake Guardian is severely limited.
The showers, sinks, washing machines and toilets drain into a holding tank (for later transfer ashore).

The following guidelines are set forth to ensure conservation of resources:

1.      Flush toilets only when required for human waste; all other disposable materials should be
       placed in a waste container.  Do not place sanitary napkins or any other foreign articles in
       toilets.

2.      Also, please wait and make sure  your  toilet finishes flushing after use.  Because our
       toilet system uses lake water and is sometimes loaded with sand particles,  the valves
       occasionally do not close  and we will have a toilet that continually flushes. As a result, the
       holding tank will fill prematurely. Call 911 (Bridge) to report a running toilet, leaking shower,
       basin, or any other leaking pipe.

3.      Due to limited sanitary capacity, please limit your showers to one per day and conserve water
       where possible. Each shower head is equipped with a mixing valve.  In order to control the
       water temperature turn  the  handle  counterclockwise to increase  the temperature and
       clockwise to decrease  the temperature.

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CLEANLINESS

Cleanliness of the vessel is an "all hands" evolution.  Our limited crew size demands that each person
on board do his share to assist in maintaining clean,  neat and orderly conditions. Personnel assigned
to each space are responsible for the cleanliness of that space, including staterooms. Public areas of
the vessel (heads, passageways, mess area, etc.) are assigned to members of the crew for cleaning.
However, when you leave the lounge area, please remove all trash and return books, games, snacks,
etc. to the racks.  Leave the lounge ready for the next person. Your cooperation in this matter will
be appreciated.

Upon final departure, please do not remove or take any items belonging to the ship as a souvenir.
We are held accountable for all government property, and the next group to sail with us may need
to use these items.

PERSONAL POSSESSIONS

Seaward  Services, Inc. cannot be responsible for  the loss of personal effects.  Items of significant
value should be left ashore, or given to the ship's Master for  storage in the ship's safe.

COMMUNICATIONS

Daily radio  or cellular telephone contact is made with the GLNPO office and occasionally, with
Seaward Service's office.  The Master can handle routine or emergency traffic via the marine operator
as may be required. Personal and business calls will be made by credit card or collect only, unless
prior arrangements have been made to pay for this service.

Use of the cellular phone, when activated and in a coverage area, is for government and business use
only.  Permission to use the phone must be obtained from the Master or Chief Scientist and all calls
must be logged.

SMOKING

In an effort to maintain the enclosed spaces of the ship as "sweet smelling" as possible, we insist that
smokers restrict this habit to designated outside areas.

FUELING

There is absolutely no smoking allowed when we a/e taking on fuel. Whenever the vessel is taking
on fuel, as evidenced by the red "bravo" flag flying by day, or by a red light on the mast at night, the
smoking lamp shall be extinguished throughout the vessel. Announcements shall be made to further
warn personnel when fueling operations are being conducted. When completed, the smoking lamp
will be re-lit.

ALCOHOLIC BEVERAGES AND ILLEGAL  DRUGS

                                           8

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 Alcoholic beverages and illegal drugs in any form will not be consumed or brought aboard by any
 persons. Any personnel found to be in violation of this regulation will have the substance confiscated
 and upon arrival at the next port will be discharged from the vessel.  Further, any person found to be
 in violation shall be reported to the local authority and prosecuted to the full extent of the law.
FIREARMS

Firearms are prohibited onboard the R/V Lake Guardian at all times.

TOURS OF THE VESSEL BY THE PUBLIC

The R/V Lake Guardian is funded by taxpayer dollars and an effort is made by USEPA and Seaward
Services, Inc. to insure that the vessel is available to the general public on a not-to-interfere basis with
scientific operations, repair and maintenance work or crew privacy.  With this in mind, scientific and
crew personnel desirous of providing individuals a tour of the vessel must first check with the Master
or the USEPA Project Scientist before conducting such a tour. We request that tours be conducted
only during the hours of 0900-2000, with the exception of mealtimes.   Only public areas (lab
passageways, pilothouse, open deck areas) of the vessel may be shown. Berthing areas are off limits
for tours, except for unoccupied cabins which may have  open doors for viewing purposes only.
Visitors should not enter the cabins.  Ship's company abrogating this simple courtesy will be subject
to immediate discharge from the vessel.

IN CONCLUSION

This Welcome Aboard handout has been provided for your use in becoming familiar with the Lake
Guardian and its operation.  Although it is a relatively  large  ship, after several weeks at sea it can
sometimes seem mighty small, especially to individuals  without any previous seagoing experience.
However, if we all work as a team and everyone does his/her own job (and Mother Nature smiles
upon us), we can all expect another rewarding experience,  both personally and professionally.

Once again, WELCOME ABOARD!

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                                                      SECTION: APPENDIX Q
                                                      VERSION: FINAL/MAY 1997
                                                      PAGE: COVER
APPENDIX Q: MARINE SANITATION STANDARD OPERATING PROCEDURE

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                                                                   Attachment  1

           R/V LAKE GUARDIAN STANDARD OPERATING PROCEDURE
                      FOR FREE AVAILABLE CHLORINE TEST
                           OF POTABLE WATER SYSTEMS

It has been recommended by the Naval Hospital Occupational Health and Preventative Medicine
Department that Free Available Chlorine (FAC) tests shall be performed aboard the Lake Guardian.
This test will be performed by the E/T or M/T prior to shore to ship connection of the potable water
hose. In addition, this test will be performed 30 minutes after the water has settled in the vessel's
tanks and is to be  collected at the riser.

In accordance with the "Manual of Naval Preventative Medicine Standards" a FAC level of 0.2 ppm
should be maintained in the vessel's tanks, and a trace level reading should be maintained throughout
the ship's distribution system.

Free Available Chlorine Test  Procedure for Potable Water

This procedure uses the DPD (N,N-Diethyl-p-Phenylenediamine) Method with 13 mm viewing depth
tubes in the Orbeco-Hellige Pocket Comparator No. 605.

1.      After letting the line  flush for 1 -2 minutes, fill one of the test kit tubes with water to 10
       ml line. Insert  into the top left  side opening of comparator (next to hinge).  This tube
       will serve as compensation for sample color or turbidity (blank).

2.      Rinse a second tube with water, leaving just enough to moisten tablet when added.

3.      Add one DPD No. 1 Tablet and crush with plastic stirring rod.

4.      Remove rod. Fill tube with water sample to 10 ml line and mix using stirring rod.  Insert
       this tube into the right-hand opening in the comparator.

5.      Place DPD disc in comparator, revolve until a match is obtained between one of the glass
       standards  and the test solution.  The result in parts per million (milligrams per liter) of
       FREE AVAILABLE CHLORINE is read directly from the figure appearing in the upper
       opening at the right side of the front cover of the comparator.

HIGHER RANGE EXTENSION
Chlorine residuals greater than the highest value on a color disc may be easily tested by diluting the
sample with distilled or deionized water. For example, the DPD tablet is dissolved in 5 ml of the
distilled or deionized water, to which sample is added to the  10 ml line.  The reading obtained, is
multiplied by the dilution factor 10/5 = 2.  Likewise, if 2 ml of sample is added to 8 ml of distilled
deionized water, in which a tablet has been dissolved, the reading obtained is multiplied by 10/2 = 5.

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DIRECTIONS FOR USE OF THE ORBECO-HELLIGE POCKET COMPARATOR

To insert a Color Disc, open the front cover of the comparator and place the center of the disc on the
ring with the numbers facing outward. With the cover closed, the numbers are so arranged that only
one result number is visible when a standard is completely centered in the field of view.

The observation fields for colorimetric comparison are seen through the two central openings.  These
appear as concentric half-fields through the Magnifying Prism Attachment. The right-hand field shows
the tube containing treated sample which is placed in the right-hand opening at the top of the
comparator. The left-hand field shows one of the color standards and the tube containing the "blank"
(usually clear water or untreated sample - sometimes called the "compensation tube"), which is placed
in the left-hand top opening.  This "blank"  eliminates possible errors due to color or turbidity in the
sample fluid, and the preparation is covered in the procedure supplied with each Color Disc.

In tests where only 5 ml of sample is utilized, insert the tube only sufficiently to permit the color of
the solution to be seen in the  right-hand field.   After the tubes  are in the comparator, color
comparison is accomplished by rotating the disc so that one standard after another is brought into the
left-hand field, and until a color match is achieved.

When using a Pocket Comparator equipped with a Magnifying Prism Attachment, the color fields
should be viewed with the eye in line with the center of the lens at approximately 10 inches viewing
distance. The half-fields should be free of shadow effect, which is caused by off-center or oblique
observation. For best results, rest the eyes between readings, preferably by viewing a gray or green
background. CAUTION: DO NOT PLACE THE EYE CLOSE TO THE PRISM OR PROLONG
OBSERVATION BEYOND 10-15 SECONDS.

When a color match is obtained with one of the Standards, the result is read directly from the figure
seen through the opening at the right side of the front cover.  If the color of the test solution is
intermediate between two Standards, the value may be estimated by interpolating between the
corresponding values.

For comparisons in daylight, northern exposure is best, but any indirect lighting from outdoors usually
is satisfactory.  Direct
sunlight should always be avoided, and while making color comparisons, the opal glass at the back
of the comparator should directly face the light.  Care should be taken to avoid nonuniform
illumination of the two light fields from pronounced shadows.
Cleanliness is most important in all colorimetric measurements. Observing some simple cleanliness
guidelines will save time and effort. The Comparator, Prism Attachment and Color Disc  should
always be kept clean.  Should solutions be accidentally spilled in the Comparator, the back cover
should be opened and interior cleaned,  and the interior of the  opal  glass cleaned periodically
regardless. Drying the outside of the Precision Tube before insertion will help minimize  dirt or
corrosive effects on the interior parts. Should a squeaking noise occur when the front or back cover
are opened, it can be corrected by placing a drop of oil along the inner hinge rim.

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                                                                           Attachment 2

                               STANDARD OPERATING PROCEDURE
                    FOR BACTERIOLOGICAL TESTING OF POTABLE WATER
                                    (PRESENCE/ABSENCE TEST)

 Product Use;
 Colilert is used for the simultaneous detection, specific identification and confirmation of total coliforms and E. coli
 in water.

 Principle;
 Colilert is based on the Defined Substrate Technology (DST).  DST utilizes indicator-nutrients which cause target
 microbes contained the sample and incubated in the DST reagent system to produce a color change (or another
 signal,  i.e., fluorescence), both indicating and confirming their presence.  The indicator-nutrient is cleaved by the
 target microbe which metabolizes the nutrient and frees the indicator to express a specific color. The growth and
 reproduction process of the target microbe if fueled by the nutrient.

 Colilert is a specially designed reagent formulation of salts, nitrogen and carbon sources that are specific to total
 coliforms.  It provides specific indicator nutrients: ONPG (0-Nitrophenyl-p-d-Galactopyroniside) and MUG (4-
 Methyllumbelliferyl-p-d-Glucuronide) for the test microbes,  total coliforms and Escherichia coli. As these nutrients
 are metabolized, yellow color (from ONPG) and  fluorescence (from MUG) are released confirming the presence of
.total coliforms and E. coli respectively. Non-coliform bacteria are suppressed and cannot metabolize the indicator
'nutrients.  Consequently, they do not interfere with the specific identification of the target microbes during the test
 incubation period.

 Performance Characteristics;
 Total coliforms and E. coli are specifically and simultaneously detected and identified at 1 CFU/100 mL of sample,
 in 24 hours  or less, by inoculating the reagent with  the water sample and incubating  it.  No further sample
 manipulation or testing is required.  Field and in-house data show Colilert to be sensitive and  specific for  the
 detection of total coliforms and E. Coli at the 1 CFU/100 mL level in  water  samples with as many as 20,000
 heterotrophic bacteria present  per mL.

 Materials Required:
 1.      35 ±0.5°C Incubator
 2.      Long wavelength (365nm) ultraviolet lamp.
 3.      Color and fluorescence comparator.
 4.      120 mL sterile high clarity polystyrene vessels with  100 mL fill line  and screw caps with sodium thiosulfate
 5.      Colilert reagent tubes.  Each tube provides reagent sufficient for 100 mL water sample.

 Storage and Shelf Life;
 Store and 4-30°, away from light in a cool, dry environment. Colilert is stable under these conditions for at least 15
 months from date of manufacture.
                                                 Page 1

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Sample Collection:
When the sample is collected, leave ample air space in the bottle (at least 2 5 cm or 1 in.) to facilitate mixing of the
sample by shaking, preparatory to examination.  Care must be exercised to take samples that will be representative
of the water being tested and to avoid contamination of the sample at the time of collection or in the period before
examination.

The sample bottle shall be kept unopened until the moment it is to be filled.  Remove the cap as a unit, taking care
to avoid soiling. During sampling, do not handle the cap or neck of the bottle and protect them from contamination.
Hold the bottle near the base, fill it without rinsing, replace the cap immediately and secure the cap.

The samples of water should be collected once a week from two locations throughout the ship. One sample should
be taken from a forward or aft tap on the focsle deck, and the other sample should be taken from a tap on the 01 deck
at the opposing end (fore or aft) of where the first sample was taken.  In addition, one sample per month should be
collected from alternating potable water tanks in the engine room.  The tap should be opened fully and the water
allowed to run to waste for 2-3 minutes or for a time to permit clearing of the service line. The flow from the tap
should be restricted to one that will permit filling the bottle without splashing.  Leaking taps that allow water to flow
over the outside of the tap must be avoided as sampling points.

Procedural Notes:
1 .     Avoid touching or otherwise contaminating the reagent or the inside of the reaction vessels or caps.
2.     Colilert is for analytical testing only.
3.     Adhere to good laboratory practice throughout the testing procedure.
4.     Thoroughly mix all  samples immediately before inoculating.
5.     Never autoclave Colilert  prior to use.  This process will destroy the reagent system, which is heat labile.
6.     Avoid prolonged exposure of the inoculated Colilert to direct sunlight.  The indicator compounds may be
       hydrolyzed, creating a false-positive (yellow) result.
7.     After  inoculation,  Colilert should be incubated for 24 hours at  35  ± 0.5°C.  Avoid incubation at this
       temperature beyond 28 hours because heterotrophic bacteria present my overcome the suppressant systems
       after this time, yielding a false positive.  Yellow color after the 28 hour incubation period should be verified
       or the sample repeated.
8.     Colilert is a primary water test. Colilert performance characteristics do not apply to samples altered by any
       form of pre-enrichment or concentration.  This includes any method such as growth on a membrane filter or
       growth in lactose based broth in which there is a non-specific growth enhancing step, or any pre-filtration
       method such as filtering the sample through a membrane filter and then using the filter to inoculate in Colilert.
       a.      Do not transfer colonies or cultures pre-grown in any enrichment media to Colilert. Colonies grown
              in such non-specific media may or may not be coliforms.   Colilert's suppressant reagents may be
              overloaded by transferring such heavy inocula of certain very weak p-galactosidase containing non-
               coliforms (e.g., some Aeromonas and Psendonmonas), causing a false positive total coliform result.
               Similarly, transfer of  high numbers of other heterotrophs (for  example, Flavobacterium) can cause
               a false positive p-glucuronidase fluorescence and inaccurate indication that E. coli is present. While
               one would not normally expect to encounter such extremely high levels of heterotrophs in a water
               sample, pre-enrichment could produce them.
                                                 Page 2

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       b.      Do not pre-filter a sample and then place that filter in Colilert.  The filtration step can concentrate
              coliforms but also non-coliforms, heterotrophs, particulates, and certain chemicals (divalent cations,
              heavy metals, etc.) which can overlay and suppress coliforms adversely affecting the sensitivity of the
              test. Furthermore, coliform bacteria can become trapped in the filter, restricting their access to the
              indicator-nutrients in the Colilert reagent and their subsequent growth and detection.
9.      Do not dilute sample in buffered water for addition to Colilert. Colilert is already buffered and additional
       buffer compounds can adversely affect the growth of the target microbes and test performance.
10.    If additional confirmation is desired after incubating 24 to 28 hours and reading results, transfer 0.1 mL with
       a pipet to EC + MUG or other confirmation media.
11.    Upon mixing of Colilert reagent with the sample, a transient blue color may appear in containing 40-50 times
       the amount of free chlorine normally found in drinking water. The sample should be considered invalid and
       testing discontinued.
12.    High calcium salt concentrations in certain waters may cause a slight precipitate. This should not affecl
       results.

Test Procedure:
1.      Aseptically open a tube of Colilert reagent and add the contents to a  100 mL water sample in a sterile,
       transparent, non-fluorescent borosilicate glass container or high clarity polystyrene sterile vessels.
2.      Shake vigorously be repeated inversion to aid dissolution of the reagent.  Some  particles may remain
       undissolved.  Dissolution will continue during incubation.
3.      Incubate reagent/sample mixture at 35 ± 0.5°C for 24 hours.
4.      Read the reaction at 24 hours.  If yellow color  is seen, check for fluorescence.  Color should be uniform
       throughout the vessel.  If not, mix by inversion before reading.

Test Results and Interpretation;
At 24 hours, compare each reaction vessel against the color comparator dispensed into an identical vessel.  If no
yellow is observed, the test is negative for total coliforms and E. coll. If the sample has  a yellow color greater or
equal to the comparator, the presence of total coliforms is confirmed.

If yellow is observed at 24 hours, check each vessel for fluorescence by placing it three-five inches from the U.V.
lamp.  Observe for fluorescence in a dark environment.  If fluorescence if vessel(s) is greater or equal to fluorescence
of the comparator, the presence of E. coli is specifically confirmed.

The comparator is the lowest level of yellow and fluorescence which can be considered positive. A typical positive
test is much more intense than the comparator.

If a sample is yellow after 24 hours if incubation, but slightly less than the positive comparator, it may be incubated
up to an additional 4 hours.  If the sample is coliform  positive, the  color will intensify.  If it does not intensify.
consider the  sample negative. Some water samples containing humic material may have an innate color. If a watei
sample has background color, compare inoculated Colilert vessel to a control blank of the same water sample.

If an inoculated Colilert vessel is inadvertently incubated over 28 hours - the following guidelines apply: No yellov,
after this incubation period should be verified or the test repeated.

                                                 Page 3

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Quality Control Procedures;
Routine quality control should be conducted on each lot of Colilert received to ensure integrity and proper product
performance.
Recommended Procedure:
 1.     Aseptically empty contents of a tube of Colilert into a sterile vessel containing 100 mL sterile distilled water
       Mix thoroughly to aid dissolution.
 2.     Aseptically aliquot one-third of the sterile water/Colilert mixture into each of 3 sterile transparent (but non-
       fluorescent) borosilicate glass water collection/culture vessels or equivalent.
 3.     Label the vessels "Escherichia coll" "Klebsiellapneumoniae" and "Psettdomonas aeruginosa" respectively.
 4.     Touch a sterile inoculating loop to an  18'-24 hour pure culture slant of one  of the bacteria listed.
       (Alternately, ATCC strains below may be used as source of inoculum).
 5.     Transfer the inoculum to the appropriately labeled Colilert vessel.
 6.     Repeat steps 4 and 5 for the two remaining control organisms.
 7.     Incubate the inoculated Colilert vessels at 35 ± 0.5°C for 24 hours.
 8.     Results should be observed within 24 hours as fo"ows:

E. coli - Yellow and fluorescent (ATCC #25922, 11775 or equivalent).

K. pneumoniae - Yellow, no fluorescence (ATCC #9997 or equivalent).

Pseudomonas aernginosa - No color, no fluorescence (ATCC #10145 27853 or equivalent).

ATCC (American Type Culture Collection,  1-800-321-0207)                                              41
                                                Page 4

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    R/V LAKE GUARDIAN FREEZER/REEFER LOG
            MONTH    YEAR
DAY  FREEZER  AM / PM
REEFER  AM / PM
  10
  11
  12
  13
  14
  15
 16
 17
 18
 19
 20
 21
 22
 23
 24
 25
 26
 27
 28
 29
 30
 31

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                        R/V LAKE GUARDIAN
                      ICE MACHINE CLEANING
DATE
DRAINED
DISINFECTED
INITIALS

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                                                      SECTION:  APPENDIX R
                                                      VERSION:  FINAL/MAY 1997
                                                      PAGE:  1 of 15
APPENDIX R:       U.S. EPA GREAT LAKES NATIONAL PROGRAM OFFICE
                    R/V LAKE GUARDIAN HAZARDOUS MATERIALS /
                    HAZARDOUS WASTE CONTINGENCY PLAN

                                 TABLE OF CONTENTS

       I.      DESCRIPTION OF FACILITIES AND ACTIVITIES                  3

       II.      HAZARDOUS MATERIALS STORAGE                           4

       III.     HAZARDOUS WASTE STORAGE                                4

              A.   Satellite Storage                                           4

              B.   Primary & Interim Storage Area                              5

       IV.     IMPLEMENTATION OF THE CONTINGENCY PLAN                5

              A.   General Procedures                                         5

              B.   Hazardous Waste Spill Response Procedures                     6

                   1.  Liquid Acid Spill                                       6

                   2.  Liquid Basic Spill                                      7

                   3.  Organic Solvent Spill Containing Less Than
                      50 ppm  PCBs                                         8

                   4.  Organic Spill Containing More Than 50 ppm
                      and Less Than 500 ppm PCBs                            9

                   5.  Other Liquid Spill                                      12

                   6.  Solid Spill                                            13

                   7.  Mercury Spill                                         14

                   8.  Radioactive Material Spill                                15

             C.    Fire or Explosion Resulting in Fire                            17

             D.    Medical Treatment                                         17

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                                                          SECTION:  APPENDIX R
                                                          VERSION:  FINAL/MAY 1997
                                                          PAGE:  2 of 15
ATTACHMENTS

Attachment 1:  Waste Stream Analysis

Attachment 2:  Laboratory Floor Plan

Attachment 3:  Chemical Storage by Room

Attachment 4:  Emergency Phone Numbers

Attachment 5:  Emergency Equipment Locations

Attachment 6:  Emergency Response Team

Attachment 7:  OSHA 29 CFR 1910.120 Health & Safety Plan Compliance Procedures

Attachment 8:  Notification To Emergency Assistance Authorities

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                                                          SECTION:  APPENDIX R
                                                          VERSION:  FINAL/MAY 1997
                                                          PAGE:  3 of 15
                          R/V LAKE GUARDIAN LABORATORIES
                HAZARDOUS MATERIALS/WASTE CONTINGENCY PLAN
This contingency plan  has  been developed to address emergencies which may occur relating  to
hazardous waste generated in the laboratory and hazardous materials used in  the laboratory.  The plan
is designed to minimize hazards to human health and the environment from fires, explosions, or any
unplanned  sudden or inadvertent release  of hazardous  waste  or hazardous materials affecting the
vessel, air,  soil, or surface water.

Copies of this contingency plan have been distributed to  emergency assistance authorities  indicated  in
Attachment 8.
I.  DESCRIPTION OF FACILITY AND ACTIVITIES

The Lake Guardian  is a research  vessel owned  by the  U.S.  Environmental Protection Agency and
operated  by the EPA's Great Lakes National  Program  Office (GLNPO) in conjunction with  other
scientific monitoring  and surveillance programs.  The home port of the Lake Guardian is located at 24
Sixth  Street in Bay City, Michigan.  The ship has been equipped with three permanent laboratories
and can accommodate as many as eight portable  laboratories.  Sailing operations are administered by
an 13  member crew and, at any one time, as  many  as 29 scientists may  be  on board to conduct
research.

The Lake Guardian  laboratories provide research services supporting  the Clean  Water Act and the
Great  Lakes  Water Quality Agreement  between the U.S. and Canada.   Most water  and sediment
samples taken from the Great Lakes are prepared and analyzed in the laboratories aboard ship.  A
variety of chemicals  are stored on the ship to facilitate appropriate testing.  Most of these chemicals
are in small quantity.

The laboratories may generate specific hazardous  wastes depending on the type of analysis being done
on board the  vessel.  Generating  a combined total of less than  100  kg. of hazardous  waste per month,
the laboratories are considered under the Resource Conservation and Recovery Act (40 CFR 260-263)
to be a conditionally  exempt waste generator. The laboratories, however, are prepared to comply with
the RCRA small quantity generator requirements.  All such wastes are designated for off-site disposal
and consist of the following waste streams:

       1.  Acidic waste from metals preparation and metals standards. This waste
           contains hydrochloric acid and/or nitric acid and metals.

       2.  Basic or  neutral waste from chlorine  or ammonia analytical systems. This waste contains
           sodium hydroxide and/or phenol.

       3.  Organic solvent waste containing less than 50  mg/1 PCBs.

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       4.  Low level radioactive waste.

       5.  Acidic wastes from Dissolved Oxygen studies and Dissolved Organic Carbon studies.

       6.  Formaldehyde from Biology Studies

       See Attachment 1  for waste stream analysis.


II.  HAZARDOUS MATERIAL STORAGE

Hazardous  materials are stored  in  the  Hazardous Materials  Locker  and in the laboratories.   The
laboratories maintain  small  quantities of reagents and chemicals used on  a daily basis.   All other
storage is in the locker. Refer to Attachment 2 for a diagram of the  laboratories and Attachment 3
for specific storage locations.


III. HAZARDOUS WASTE STORAGE

Hazardous  wastes are  stored in three areas consisting of the satellite storage area, the primary storage
area, and the interim or secondary storage.  Storage and labeling methods for each area is as follows:

       A.  Satellite Storage

           Hazardous  waste generated  during  preparation and  analytical  operations is  temporarily
           stored in the laboratory where it is generated.

           The  acidic  and  basic  hazardous wastes  are stored  in  polyethylene  containers and  the
           organic solvent  hazardous waste is  stored in flammable liquid  storage containers.  Each
           waste container  is labeled and coded with NFPA  label or  colored  label.  The wastes are
           color-coded as follows:

               1.  Red - Acid waste from metals preparation and metals standards;

               2.  Blue - Basic or neutral waste;

               3.  Purple - Organic waste solvents containing more than 50  mg/1 PCBs;

               4.  Green - Organic  waste solvents containing less than  50 mg/1 PCBs.

               5.  Orange - Flammable Solvents

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       B.  Primary Storage

           The primary  storage area for  all hazardous wastes is  the Hazardous Materials  Locker.
           This room  is also the storage  area  for all  hazardous materials  prior to their use in the
           laboratories.  The locker  is divided into two  storage areas, one  containing the storage
           cabinets for corrosives and poisons and the  other containing the cabinets for  flammables.
           The storage areas  are separately diked and a  dry  chemical fire suppression system has
           been installed.

       C.  Interim or Secondary Storage

           Secondary  storage at a  land  facility has  been  proposed.   Until that storage  area is
           completed,  interim storage is currently maintained  at the Sears  Warehouse in Bay City,
           Michigan.  Transport of hazardous waste or materials to  interim storage may be performed
           only by EPA  personnel following the established transport guidelines.
IV. IMPLEMENTATION OF THE CONTINGENCY PLAN

The contingency plan will be implemented immediately whenever there is a fire,  explosion, or release
of hazardous waste or hazardous materials which could threaten human health  or the  environment.
The following emergency procedures will be carried out in the event of an emergency.

       A.  General Procedures

           In the event of a release  of hazardous  waste or  hazardous materials, the Emergency
           Coordinator (EC), Captain,  and ship's Safety Committee will be notified immediately.  A
           list of emergency  response team  phone numbers are provided  in Attachment 6.   The
           Safety Committee  is comprised of the  Captain, EPA Supervisor, the Chemical Hygiene
           Officer  (CHO),  and  Group  Supervisor if an outside  group  is using the ship.   The
           Emergency Coordinator will  identify the character,  source,  and amount of any released
           material and determine  whether the release  can  be  abated  by ship  emergency response
           personnel.  The  Emergency Coordinator must  advise the  Captain and Safety Committee
           members of the  conditions  relating to the release event.  If the Emergency Coordinator
           concludes that qualified ship personnel  can abate the release,  he will specify the  task
           assignments to qualified emergency response team personnel Attachment 6.

           Response  activities involving  hazardous  material/waste  releases must  be performed  in
           accordance  with  Attachment  7, OSHA  29 CFR 1910.120  Health &  Safety   Plan
           Compliance.

           If the Emergency  Coordinator determines that the  release cannot be controlled by  ship
           personnel and/or the health of ship personnel is  endangered, the Captain  will implement
           the evacuation plan (See "Occupant Emergency Plan" as it appears in Appendix M of the
           GLNPO Health and Safety and Environmental Compliance Manual).   The Captain,  with

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    the  assistance of the Emergency  Coordinator, also has the responsibility to notify the
    GLNPO office  and appropriate authorities, such as the National Response Center and the
    Coast Guard. (See Chapter 2, Attachment 2.5 of this manual and Attachment 4 of this
    appendix.)

B.  Hazardous Materials or Waste Spill Response Procedures

    In the event of a spill, the personnel assigned to  emergency response will evaluate the
    spill,  consult the material  safety data  sheets or  waste analyses,  and  use appropriate
    procedures  and equipment for  spill clean-up.   If the chemical nature of the spill is not
    known, the  spill will  be  treated  as  a  highly  toxic  and  hazardous  material.   See
    Attachment 5 of this appendix for locations of emergency response equipment.

        1.  Liquid Acid Spill

           In the event of liquid acid  spill, the following procedures will be implemented:

           a.   Before entering the area,  response personnel will be equipped with appropriate
               personal protective  equipment,  as determined by the EC.   Equipment used in
               this situation may  include Self-Contained  Breathing  Apparatus, Tyvek suits,
               chemical resistant boots, and neoprene gloves.

           b.  If possible,  the leaking container will be moved  in such  a way that  further
               release of acid is prevented.  The container v/ill  the be diked with absorbent
               booms.   (Absorbent  booms  are  long  pliant  tubes  containing  absorbent
               material.)

           c.   The spill will be  contained through the use of absorbent booms to prevent
               further spread of the material.

           d.  Acid  neutralizing spill material such as lime chips will be sprinkled over the
               spill area and will be used until the  liquid has been neutralized.  Neutralization
               will be determined by using pH paper.

           e.   Once the liquid has been neutralized, the absorbent booms will  be placed into
               a collection drum  and covered with neutralization  material.    The solidified
               spill material  will be shoveled or scooped into the collection  drum, as well,
               using a nonreactive, non-sparking shovel.
            f.  After the spill has been contained, remaining liquid in  the  leaking container
               will be  placed in a secure container.  If it is not feasible to transfer the liquid,
               the container will be placed in a secure overpack.

            g.  Solid surfaces contaminated by the  spill, such as floors  and walls,  will  be

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           triple washed and rinsed with a commercial-grade aqueous detergent solution
           such  as  "Det-O-Jet".   "Washing"  refers to scrubbing the  area with  a fresh
           solution  used for each wash cycle.  A sufficient amount of fresh fluid, enough
           to cover the  contaminated surface completely, must be i:sed in each wash and
           rinse.  The floors will be dried with a clean cloth after each rinse.  Precautions
           must be taken  to  contain any  runoff  from the  scrubbing and  to  properly
           dispose of wastes generated during the cleaning.

        h.  The decontamination  material, wash  and rinse  solutions, and all disposable
           equipment used during the clean-up will be placed in the collection drum and
           the EC will arrange  for its transfer to a disposal site.

2. Liquid Base Spill

    In the event of a liquid base spill the  following procedures  will be implemented:

    a.   Before entering the  area, response personnel will be equipped with the appropriate
        personal  protective equipment as  determined by  the EC.    Equipment  in this
        situation   may   include   self-contained   breathing   apparatus,   Tyvek   suits,
        chemical-resistant boots, and neoprene  gloves.

    b.   If possible, the leaking container  will be moved in such a way that further release
        of material is prevented.  The  container will be diked with absorbent booms.

    c.   The spill will be contained through the use of absorbent booms to prevent further
        spread of the material.

    d.   Basic  neutralizing spill  material,  such as anhydrous citric acid granules,  will be
        sprinkled over the spill area.

    e.   Once  the  spill has  been neutralized, as determined by  pH  paper, the absorbent
        booms will  be  placed  into  a collection  drum and  covered  with  neutralization
        material.   The  solidified  spill  material  will be  shoveled  or scooped  into  the
        collection  drum using a  non-reactive, non-sparking shovel.

    f.   After the spill has been  contained, material remaining in the  leaking container will
        be  place  in  a secure container.   If it  is not feasible  to transfer the material,  the
        leaking container will be placed in a secure overpack.

    g.  Solid  surfaces contaminated by the  spill,  such as  floors and walls, will be  triple
        washed and rinsed  with a commercial-grade aqueous detergent solution  such as
        "Det-O-Jet".  "Washing" refers to scrubbing the area with  a fresh solution used for
        each wash cvcle.  A sufficient  amount of fresh fluid,    enough to cover  the
        contaminated surface completely, must  be used in each  wash and  rinse.   The
        floors will be dried  with a clean cloth  after each rinse.  Precautions must be taken

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       to  contain  any runoff from the scrubbing  and to  properly  dispose of wastes
       generated during the cleaning.

    h.  The  decontamination material,  wash and  rinse solutions,  and  all  disposable
       equipment used during clean-up will be placed in the collection drum and the EC
       will arrange for its transfer to a disposal site.

3.  Organic Solvent Spill Containing Less Than 50 ppm PCBs

    a.  Before entering the area, response personnel will be equipped with the appropriate
       personal protective equipment  as determined  by the  EC.  Equipment used in this
       situation may  include self-contained  breathing apparatus, Saranex-coated Tyvek
       suits, chemical-resistant boots, and Silvershield gloves.

    b.  If possible, the leaking container will  be moved in such a way that further release
       of solvent  is prevented. The container will be diked with absorbent booms.

    c.  The spill  will  also be contained with absorbent booms to  prevent the further
       spread of the material.  Absorbent material, such as activated charcoal or activated
       carbon, will be spread over the area  to adsorb the solvent.

    d.  Once the  liquid has  been adsorbed, the absorbent booms will be placed into a
       collection drum and covered  with absorbent material.   The solid absorption residue
       will be shoveled or scooped into the  collection drum.   The shovel or scoop used
       will be made from a non-sparking material, such as brass.

    e.  After the spill has been contained,  the remaining liquid  in the leaking container
       will be transferred to a secure container.  If it  is not  feasible to transfer the liquid,
       the container will be placed in  a secure overpack.

    f.  Solid surfaces  contaminated  by the  spill, such as floors and walls, will be triple
       washed and rinsed with a commercial-grade  aqueous detergent  solution such as
       "Det-O-Jet". "Washing" refers to scrubbing the area  with a fresh  solution used for
       each wash cycle.   A sufficient  amount  of fresh   fluid,   enough  to cover the
       contaminated  surface  completely, must be  used in  each wash  and  rinse.  The
       floors will be dried with a clean cloth after each   rinse.   Precautions  must  be
       taken to contain any  runoff  from  the  scrubbing and  to properly dispose of wastes
       generated during the cleaning.

    g.  The  decontamination material,  wash and  rinse solutions,  and  all  disposable
       equipment  used during the clean-up will be  placed in the collection drum and the
       EC will  arrange for its transfer to disposal site.

4.  Organic Solvent Spili Containing More Than 50 ppm and Less Than 500 ppm PCBs

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Materials  containing more than 50  ppm  and less  than  500 ppm  of polychlorinated
biphenyls  (PCBs) are  used  only  occasionally and in small quantities  in  the  Lake
Guardian  laboratories.   Since the  total amount of  PCBs that could be purified  from
the  solutions on board  is  less  than  one  pound,  the  follov ing  procedures are
appropriate and will be implemented for clean-ups.

a.   Before entering the area,  response personnel will be equipped with the appropriate
    personal  protective  equipment.  Equipment used  for  this particular release  will
    include self-contained breathing apparatus, Saranex-coated Tyvek suits, chemical
    resistant boots, and Silvershield gloves.

b.   If possible, the leaking container will  be moved in such a way  that further release
    of material is prevented.  The container will be  diked with absorbent booms.

c.   If the  emergency response team is  unable to determine the boundaries of the  spill,
    a statistically valid sampling survey will be performed in the area to determine the
    extent of the  spill.

d.   The spill will be contained through the use of absorbent booms to prevent further
    spread of the material.  Solvent absorbent material,  such as activated charcoal or
    activated carbon, will be sprinkled over the spill  area.  Once  the spill has  been
    absorbed,  the absorbent booms will be  moved  to  a  collection  drum  and covered
    with absorbent.   The solidified spill material will be shoveled or  scooped into the
    collection drum using a non-sparking shovel, such as  one made of brass.

e.   After the spill has been contained, material remaining in the leaking container will
    be placed in  a secure container.  If it is not feasible to  transfer  the material, the
    leaking container will be  placed in a secure overpack.

f.   Solid  surfaces contaminated by the spill, such  as  floors and walls, will be triple
    washed and rinsed with  a  commercial-grade aqueous detergent  solution such as
    "Det-O-Jet".  Washing means  scrubbing the area with a material  in which PCBs
    are  at least 5%  soluble.   A sufficient amount of PCB-free fluid,  enough to cover
    the  contaminated   surface  completely,  must be   used   in  each   wash/rinse.
    Precautions  must be taken to contain  any  runoff  from  the  scrubbing  and to
    properly dispose of wastes generated during the  cleansing.

g.   After the solid surfaces,  contaminated by the spill,  have been triple washed/rinsed,
    the  surfaces will be  sampled to determine the  quantity of PCBs  remaining.   The
    sampling will be done in accordance with 40 CFR Part 761.123.  The testing will
    ensure that the area has been cleaned to 10 ug/100 square centimeters if the  spill
    occurred  in a work area,  or 100 ug/100  square  centimeters if the  spill occurred in
    the  flammable storage room.

h.   At the completion of clean-up, the EC  will document the clean-up with  records

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                  and  certification of  decontamination.   The  records  and  certification  will  be
                  maintained  for  a period  of at least five years by the EC.   The records and
                  certification will consist of the following:

                  1)  Identification of the source of the spill (e.g., type of equipment);

                  2)  Estimated or actual date and time of the spill;

                  3)  The  date  and time clean-up was completed or terminated (if clean-up was
                      delayed by  emergency  or adverse  weather: the nature and duration of the
                      delay);

                  4)  A brief description of the spill location;

                  5)  Preclean-up  sampling data used to establish the spill boundaries if required
                      because of insufficient visible traces,  and a brief description of the sampling
                      methodology used to establish the spill boundaries;

                  6)  A brief description of the solid surfaces cleaned and of the wash/rinse method
                      used;

                  7)  A certification statement  signed by  the  responsible party stating  that the
                      cleanup requirements have been met and that the information contained in the
                      record is true to the best of his/her knowledge.

                    •    While not required  for  compliance with  TSCA  regulations,  the  following
                        information would be useful if maintained in the records:

                      1)      Additional pre- or post-cleanup sampling;

                      2)      The estimated cost of the cleanup by rnanhours, dollars, or both.

Due to  the quantity  of PCBs used  and stored, it is extremely unlikely that more  than one pound of
PCBs could be spilled aboard the Lake Guardian.  Specific procedures  to address this type of spill are
not contained in this contingency plan.   In the event that such a spill occurs,  the Office of Pesticides
and  Toxic Substances  will  be  contacted to  obtain guidance for appropriate  cleanup measures in the
shortest possible time after discovery of the spill,  but in no  case  later than 24 hours after discovery.
Additionally, clean-up  of more than one  pound of PCBs will be conducted in accordance with 40 CFR
Part 761.125.  Spills involving more  than  10 pounds of PCBs will be immediately reported  to the
National Response Center at  1-800-424-8802.

           5.  Other Liquid  Spill

           In the event  of a spill involving a liquid that is not a base, acid,  or solvent, the following
           procedures will be used:

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    a.   Before entering the area, response personnel will be equipped with the appropriate
        personal  protective equipment.   Equipment used  in  this  situation  may include
        self-contained  breathing apparatus, Saranex-coated Tyvek suits, chemical- resistant
        boots, and Silvershield gloves.

    b.   If possible, the leaking container  will be moved in  such  a way that further release
        of liquid is prevented.  The container will then be diked with absorbent booms.

    c.   The spill will  be contained with absorbent booms to prevent the further spread of
        the material.   Adsorbent material such as activated charcoal or activated carbon
        will be spread  over the area to adsorb the liquid.

    d.   Once  the  liquid has been adsorbed, the absorbent booms  will be removed  to  a
        collection drum and covered with absorbent material.  The solid, resulting from
        absorption, will be shoveled or scooped into the  collection drum.  The shovel or
        scoop used will be made of a non-sparking material, such as brass.

    e.   After  the spill has  been  contained, the remaining liquid  in the leaking container
        will be transferred  to a secure container.  If it is not feasible to transfer the liquid,
        the container will be placed in a secure overpack.

    f.   Solid  surfaces  contaminated by the  spill, such as floors and  walls, will  be triple
        washed and rinsed  with  a commercial-grade  aqueous detergent  solution such  as
        "Det-O-Jet". "Washing" refers to scrubbing the area with a fresh solution used for
        each  wash cycle.    A  sufficient  amount  of fresh fluid,  enough  to cover  the
        contaminated  surface completely, must  be  used in each wash  and rinse.   The
        floors  will be dried with  a clean cloth after each rinse.  Precautions must be taken
        to   contain any runoff  from the scrubbing and to properly dispose of wastes
        generated during the cleaning.

    g.   The decontamination  material,  wash,  and  rinse  solutions,  and  all  disposable
        equipment used during the cleanup will be placed in the collection drum and the
        EC will arrange for its transfer to disposal site.

    h.   If the  spilled liquid is mutagenic, teratogenic,  or carcinogenic, the contaminated
        area will be sampled to determine the quantity of chemical remaining on the solid
        surfaces.   The  sampling will be  performed  in  a statistically valid  manner.  No
        detectable traces of chemical should remain in the area.

6.  Solid Spill

In  the event  of a  spill involving a solid hazardous chemical  that  is not water or  air
reactive, the following  procedures will be used:

    a.   Before entering the area,  response personnel will be equipped  with the appropriate

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       personal protective  equipment.  Equipment  used in this  situation may include
       self-contained breathing apparatus,  Saranex-coated Tyvek suits, chemical- resistant
       boots, and Neoprene gloves.

    b.  The leaking container will be picked up and placed in a collection drum.

    c.  Using a broom and  dustpan  made  of non-reactive materials, the spilled solid will
       be swept up and placed in the collection drum.  Care will be taken to sweep up as
       much of the spilled material  as possible.

    d.  Any remaining spill material will be picked up using a damp cloth.  The  cloth and
       contaminated broom should be disposed of as  solid hazardous waste.

    e.  Solid surfaces contaminated by the spill, such as floors and walls, will be triple
       washed and rinsed with  a commercial-grade  aqueous  detergent solution such as
       "Det-O-Jet".  "Washing" refers to scrubbing the area with a fresh solution used for
       each  wash  cycle.   A sufficient   amount of fresh  fluid,  enough to  cover the
       contaminated  surface  completely,  must be used in each wash and  rinse.  The
       floors will be dried with  a clean cloth after each  rinse.  Precautions must be taken
       to   contain any  runoff  from  the  scrubbing  and to properly  dispose  of wastes
       generated during  the cleaning.

    f.  If the spilled solid is mutagenic, teratogenic or carcinogenic, the contaminated area
       will be  sampled to determine  the quantity  of  material remaining on  the  solid
       surfaces.  No detectable traces of chemical should remain in the area.

7. Mercury Spill

In the event of a mercury spill, the following procedures  will be implemented:

    a.  Before  entering the area,  response personnel will  remove all gold, copper, or silver
       jewelry  and be  equipped  with the appropriate personal  protective  equipment.
       Equipment used  in  this situation  will include self-contained breathing  apparatus,
       Saranex-coated Tvvek suits,  chemical-resistant boots, and Silvershield gloves.

    b.  If possible, the leaking container will be  moved  in such a way that further release
       of mercury is prevented.   The container will  then be placed in a hard-sided waste
       collection container.

    c.  The mercury will be collected  in one area through the use of wooden spatulas.

    d.  Following collection using a spatula, an aspirator will be used to retrieve as much
       of the  material  as possible.   The  material will  be placed  in  a polyethylene
       collection bottle.

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    e.   Small droplets of mercury that cannot be collected through the use of an aspirator
        will be picked up with a mercury absorbent, such as "Cinnasorb". If "Cinnasorb"
        is used, the  area should be covered at least twice with the absorbent  paste.  The
        waste paste will be placed in a wide-mouth polyethylene bottle and allowed to dry
        before the bottle is ciosed (the bottle may then be placed in the collection drum).

    f.   Once all visible traces of mercury have been collected,  material such as "Resisorb"
        will  be sprinkled over the spill  area  to collect  small  amounts  of mercury
        inaccessible to physical  cleanup (such as cracks in tile  or cement).  The absorbent
        powder will  remain on  the spill  area for a period of one hour, then removed and
        placed in the collection drum.

    g.   Solid  surfaces contaminated by the spill,  such as floors  and walls, will be triple
        washed and  rinsed with a commercial-  grade  aqueous detergent  solution such as
        "Det-O-Jet".   "Washing" refers to scrubbing the area with a fresh solution used for
        each  wash cycle.   A  sufficient amount  of  fresh  fluid,  enough to  cover the
        contaminated surface  completely,  must be  used in  each  wash and  rinse.  The
        floors will be dried with a clean  cloth after each rinse.  Precautions must be taken
        to   contain  any runoff  from  the  scrubbing  and to  properly dispose of wastes
        generated during the cleaning.

8. Radioactive Material Spill

GLNPO laboratories are equipped with two types of radioactive  materials, Nickel-63 and
Carbon-14.  The Radiation Safety Officer (RSO) for the ship shall be notified and work in
conjunction with the Safet\  Committee members should a release of radioactive materials
occur.

Nickel-63 is contained in  sealed source devices in  laboratory  equipment. In the event that
a sealed source device is found  to be leaking, the  area will be  immediately evacuated and
the Air  and  Radiation Division will  be contacted for guidance.    Emergency response
personnel will re-enter the area ONLY under the direction of a  representative from the Air
and  Radiation Division  and  will work  to  mitigate the  hazard  under the  division's
supervision.

Water  contaminated with quantities of  Carbon-14 generated  by  GLNPO laboratories is
stored  and shipped to CRL for  disposal. In  effect, this water  is  non-hazardous according
to the Nuclear  Regulator}  Commission's regulations and  can  be disposed of via city
sewers following testing.  In the event that the water is spilled or leaks from its container
prior  to disposal  into a city  sewer  system, the spills and  leaks  will be  considered
hazardous and the  following procedures will be implemented:

    a.   Before entering the area, response personnel will be equipped with the  appropriate
        personal protective  equipment.  Equipment  used in  this situation will include
        Saranex-coated Ty\ek  suits, chemical-resistant  boots, and  rubber gloves.

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       b.  Emergency  response  personnel   shall  use  a  radiation  counter  to  assist  in
           determining the boundaries of the spill.

       c.  The spill will be contained through the use of absorbent booms to prevent further
           spread of the material.

       d.  Sawdust or  vermiculite will be sprinkled over  the spill  area.   Once the spill has
           been  absorbed, the absorbent booms  will be  moved to a collection drum  and
           covered with absorbent.  The solidified spill material will be shoveled or scooped
           into the collection drum using a non-sparking shovel, such as one made of PVC or
           brass.

       e.  Solid surfaces contaminated by the spill, such  as floors and walls, will be triple
           washed and rinsed  with a commercial-grade aqueous detergent solution such as
           "Det-O-Jet".  "Washing" refers to scrubbing the area with a fresh solution used for
           each  wash  cycle.   A  sufficient  amount  of fresh  fluid, enough to cover the
           contaminated surface  completely, must  be  used in each wash  and  rinse.   The
           floors will be dried with a clean cloth after each rinse.  Precautions must be taken
           to contain  any runoff from the  scrubbing  and to  properly  dispose of wastes
           generated during the cleaning.

       f.  After the solid  surfaces contaminated by the  spill have been triple washed/rinsed,
           the area will be wipe tested to determine the quantity of Carbon-14 remaining on
           the solid surfaces.  The RSO will ensure that  Carbon-14 readings are no higher
           than background readings taken in other areas of the laboratory.

       g.  The  decontamination  material,   wash and  rinse  solutions,  and all  disposable
           equipment used during  the cleanup will  be placed in the collection drum and the
           RSO will arrange for its transfer to CRL  for disposal.

C.  Fire or Explosion Resulting in  Fire

    Procedures for fire or explosions resulting in fire are  established by the ship's Captain and
    appear on the Watchquarter Station Bill.   Only  those  persons who have been trained in
    ship  firefighting and are  assigned to those  duties on the bill shall  take  part in the
    emergency response (see Chapter 7, Attachment 7.1 as it  appears in the GLNPO Health
    and Safety and Environmental Compliance Manual).

-------
                                                   SECTION:  APPENDIX R
                                                   VERSION:  FINAL/MAY 1997
                                                   PAGE:  15 of 15
D.  Medical Treatment

    In the event that personnel are injured during a hazardous material'•/waste emergency, the
    following procedures will be used:

        1. The  ship's Captain, in  coordination with a designated First Aid  Responder, will
          evaluate the injury  and determine whether hospital treatment is necessary.   If so,
          the  Captain will make the necessary arrangements.   Emergency response team
          members and/or designated  First  Aid Responders  will provide first  aid  assistance
          until medical help can be obtained.

        2. All   injuries,  regardless of  severity,  should  be reported to the  Captain,  Safety
          Officer, and Safety  Committee for proper accident investigation.

EPA  employees potentially exposed to toxic chemicals will be requested to undergo medical
monitoring.  This service will  be provided to EPA employees through the U.S.  Public Health
Service.

-------
                                       SECTION: APPENDIX R, ATTACHMENT I
                                       VERSION: FINAL/MARCH 1997
                                       Attachment 1 - Cover
ATTACHMENT 1 - WASTE STREAM ANALYSIS
R/V LAKE GUARDIAN HAZARDOUS MATERIAL CONTINGENCY PLAN

-------
                 03/24/92
                        39:34
MINERftL-SPRlNGS CORP
                                                                                         002
     SPRINGS Corporation
loxSOO
Vashington. Wl S3074
284-9101
                      WASTE STREAM PROFILE SHEET
rrir;i/»;oH "
        MI668QQ90317
        U.S.  E.pf
        24 Sixth sit.
Bay City.  MI   487Q6
                   Fro«hlich
 517  i     894-2803
•i:;i t-1.: i H Ei?j CCNtftAL WOHKATIOf I
       Waoro  from COP 9v«fc«tn*
                  an*1yt1q
                                                                                V¥C»_	
                                                                                ™»-' •*-,'->'
                                                                                DATE J/ll/92_
                                                                                SALFSREP
                                                       CUSTOVEH INFORMATION
                                                            LJa—r.P  ft.
                                                                                          c;
                                                  BUJWAQOflEM	230  Snnrh ngflrborn  S
                                                  	Chicr jn.	TL
                                                  OOWTACT	
                                                  PHOHE(  312  i  Ofl6-fi-,H7
                                                  FAX  (       I
                                                                           Pornxl In
                                                    iv. VOLU;JL f.ua I'AUAUin
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                                                         E 0(«JM6
                                                         a CUM
                                                         DcTuaen
                              DBXXSOUCS
                                                                                       sn^.To,s^ .j
          :SITIOM : IOTAI 5 MUST Ann UP TO ten- ,i
       Sul/utic Acid
                                   1-5
       Chromic  Acid
                                   1-5
       Water
                                  90-90
                                                 mvscitsTATt
QaiooE
BLOW
                                         vaccvrr
                                         ujjoucrswa
                                         axed     Clear Tan
                             DWLD
                             Dos
                             OMDUU
                                                 SPKfK OHAVITY ,,1 ,.04
                                                                               100
                                                           > 200
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                                                             N/A
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                                                                                           N/A
                                                                                           NX

-------
03/24/92      09:35
                                                      MINERftL-SPRINGS  CORP
                                                                               003
                     1C MJJCFHi
n GOT mmn KAMI
        "RQ"  Waata Corro»iv«  Liquid,  N.o.s.(Sulfuric  Acid, Chromic  Acid)
       ~~(D002,0007, 0009)                                                             '
mo CLAM    _ Corrosiva  Material
             UN 1760
             V0.4S4
Wttfl
             60
•JM^.^.iiMMiMffiT.na.urtir,
                      0002,0007,0000
                                         DOXW 8EAPWQ
                        QYEJ
                                                          WATER flEACTMt
                                                          BXXOOCM.
DYES
QYEJ   H NO
NTS
.;urHi-)BijviTin:< TO coHnrcr VVSPST..
RIZE UICMC 9^ra«CGRiOUTION TO UAU COWECTCNS TO 1MB WVt. SL^ COf^C
TORY Moupe«fTjL i UNOWSMNO THAT A CORHECTSD COPY wu'
                DNO
M. MfFtY DMTA JHOT (UBS) *TT*CH(0
                3  ENO

                 B.Q]UIN?ULtfflM03COftf>aurON  C.D NOEPEMXHTCOMnUCTOR
                                               WORKMQ OH KHMS OTTHE 8BBWTCR
        THAT I KAVf »»RIONAU.T EXAMINED ANO AM'AMILMH WITW TH| INFORMATION JU»WlTTE3 IN TMII A NO ALL ACCOMPANYING DOCUMENTS. 0ASEO
       Of  TMOHI INOIVIOUALS IMMIDIATILY RIS^ONSIBCI fOR OITAININQ THE iwroRMATiON. t aiuivt THAT TMJ SWBMITTSO IN^OPMATION U TRUE.
Tl AND COM«.m. ANO THAT ALL KNOWN 0« 3U3HCTIO NA^AAOS HAVK BIEN OUCLOItO.  \f TH« »OXI1 A8OVI FOB GINtRATO*
CTO" Adi CHiCXIO » WHTMM CS^TIfX THAT TWt WA«TJ CMAMACTWI2ATION U»ON WHICH MINSKAl. t^WOS CO«P BILICS IS ACCLIRAT* AXD
( THAT MY IMH.OVI* ANO I MAY 1C H{LO LIAIH UNOC^ 'EOt^AL LAW. ANQ THE LAWS Of MANY STATUS. POM TH« INPOKMAT1ON PWOVlOeO IN THIS
COMPANVINQ
                                                                               A
                                                       TITLE

-------
     03/24x92     09:36
                                                MINERAL-SPRINGS  CORP
                                                                                004
RAL SPRINGS Corporation
3x500
'ashmgton. Wl 5.3074
? 84-9101
                     WASTE STREAM PROFILE SHEET
                                                                                  10-2790
                                                                              wo*
                                                                                      PATE 3/11/9."'
        MI6680090317
 U.S.  r.p.A.. Laka Guardian
_2A Sxxth  St.	
 Bay City.   MI  46708	
XKNBU.COMTACT   P»q
nr CONTACT.
 517 )J
      894-2605
      mONE MUMEH)
                                                 CONTACT.
                                           312 )_
                          —  --     '•*  I      L
                          	.     TOR CUSTOMER USE
                                                                  U.S.  E.p.A..  Region S
                                                                   230  Sout.T  Dearborn  street
                                                                  Chicago.    •„  60606	
                                                          -Janusz
        Basic Waata
From  Cl,  NHy,  or TKN
Analytical System
                                                   tv. VOLUME .irio pncKor.ir:r.
                                                AWT FATED VOUME.
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                                                        DCTUNDEH
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                                                                            DSUIXUQUM
                    IJST Af,l> UM TO inr.
       Sodium Hydroxide
       Mtfrcuric Sul
       Phenol
                                                                           DWJD
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                                                                              a	
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                                        UOUOLAYttM      BNONf
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/

-------
                    03/24/92       09:36
                                    MINERAL-SPRINGS  CORP
                                       005
10T«»HUI*M«_    "RQ" Waste Corroatve  LiquiJ,  N.O. S . (Sodium   Hydf OXide) (P002 , 3009)
fOCUM.
 Corr;flBivc Material
*•£»»
_UN1760
                  1/0-454
ITS
    Dvts
    DYES
                                       DOXMWMMd
                             OS NO
Dm  03 NO
D«s  IS wo
Dm  El NO
WATER REACTIVE

MOLOQCAL
DYEB
       anutM ooftrauTEN TO IWA ccRracnow TO THB mi% «A^
          n. i UNOOWTANO THAT A conMCTW con
      GStw   DNO              KTIAL-
«nrr O*TA»«T 91000) XTTACHTO
                     I NO
                                   lQnNnAl.>«NCBCGRPOMTQN   C.D
                                                                WOWMQ OM KHA1/ GF THE OBCUTCH
      THAT I KUV« P«n«CNAkkY KXAMINCO AND AM PAMMllAft WITH VH» tNPOnMATtOM »U*MITT1O IN TMI« AMD »U, ACCOM^ANTINQ OOCUW«N1». BA5*O
UIMT O> fMMI INCHVtCVAUI IMMIDIATILT BUFONBIiLI FOR OBTAINING THf IMfOMMATlON. I BlUVVt THAT TM| SUMMTTCD INFORMATION (S TAufL
AND OO»M^.CT». AMO THAT ALL KNOWN CM »u»F«CTiO PVUAHO* HAVI BCEN DISCLOStO I* TM1 •Oil* A«OV« rOn QINinATOH On INOC^CNOENT
3« AHf OHCOKEO. I rUftTHCA CIHTirr THAT THI *»A»TI CHAMACTCAI2ATION UPON WHICH MIM«HA^ VniMOS COMP UrUtS 18 ACCLIIMTC AND COMPLETE.
-»AT MY tMFLOYM ANO I ««Ar II HfLO LIABLE UNOM f tOtHAL LAW. ANU IHt LAW! OF »«ANT STATEB, fOU TM« INWPRMATOH TOOVIOtO '" TMIg FO«M ANO
                                                     TTTLB

-------
                 03/24/92
                       09:37
MINERAL-SPRINGS CORP
                                       006
ML SPRINGS Corporation
oxSOO
/Mhington. Wl 53074
284-9101
                      WASTE STREAM PROFILE SHEET
                                                                              W5»_JO-2602
                                                                              D*TEj/ll/92
                                                                              SAUSftEP
                                                        viuivun mi uiir,u;iiun
        MI6680090317
U.S.  E.P.A.. Laka Guardian
34 Stxth Sc.
Bay City,   MT   4fl70fl
uotmnAt CONTACT	Pag Froghlich
3YOCKMCT.
  517 i     &94-2805
                                          NA*ecFCC»fWrr	U.S.  E.P.A., Pegion 5
                                                 3UJNOAOORESS
                                                                  230 S. Cjarbor/. 3.tee:.
                                                                 Chicaqo,   J".  60606
                                                                 Poro»i.lo
                                                       312 i   aB6-65fl?
                                                 FCB CUSTOMS ltt£
       Organic w«»t«  Colvgnta  containing
       le«ji  than 50 mq/1 PCS' s	
•NOOUCMQWMTC
               lab t«»txnq
 CWWWNVfKTOHY.
 COKTAMflTYPI
                                                        2-4 dr.
                                                        1-2 dr.
                                                        PD DflUMI
                                                        DCUM
                                                                                         5,55  qal.
                             OBUJOGLDE
                                                                            QaouLOuos
MTl
       2-Butanol
                Ethar
       Ethvl Acetate
       M«thvlene  Chlorxda
       l-Proponol
2
5
. 44
,74
%
%
30. J *
0
14
Jl
.75
.0
. 11
1,
%
%
G.9 »
19
.76
%
*
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                BUDUO
                Qaucne
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                BUD*
                                                                   DKU}
                                                                   QUORUM
                                                                   DaurEJE3
                                                                                     D
                                                                                     QHOH
                                                   _Q	  «ucxnot.
                                                    0.82  7/ml pH	
                                                    10     y  *WATTO _
                                                                                   N/A
                                        8TU»  	
                                        OXBOHI
                                        flJUPAftJ
                                                       13.QQQ
                                                                     % HAL
                                                                                 < n . 11
                HNO
                Q NO
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                                                                                           H NO
                        TOJ»
1C      1*4
•MTOT4. PI
       ft*
       tn
       C4
       flM
                                                     I/TO
                                                      N/A
                                   ORI C0flQNTFTMOt£RCE .
                            0021
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                            EXS3OOVKL
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                           osf cmcx
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                                                           oa»
                                                                                 TOP
                                                                                 (Til
                                                                                 N/A
                                                                   oo«
                   COO YKfLCHXHCi

-------
                  03/24/92      09:38
                                                     MINERAL-SPRINGS  CORP
                                                                                                   007
OOT MPPWQ NAM
                       RO
                                    Flammable Liquid,  N.O.S. (F002, FQQ3 ,r005.DQ01j
tPOcmt    Flammable  Liquid
           UN1993
vaLEQUAKTITY   1Q/4 . 54
«AKOOV«CCO«D_
fTt      PYAOAOUC     Dril   H«      OCWBIAflMQ
                      DYES   12 HO      PE3naoeM*MK
                      Qm   Q] NO      8HOCX«SK»fTTVg
9	 __^^_^_^_____-______^—
                                                             a TBS  El HO      UOLOQCM.
                                                                    E)NO
mirmi/Aiiort in tarintcr '.vans
EkMWL«*KUCXWOWTiaUOU*tfCCRMCTafflTOTHBW5^
RY flEOUKlWfTS. IUNOSWTJWO THAT A CORRKTW CCW WU
       HYES   DNO
1MTH DATA WEFT (WOW AHAOCO
                               DTES
                                    i(Si«eutswi«acoKPCJUTioK
                                                                  WORM« OH tfHMJ OTTHI OCNEHATQH
       THAT I HAV» FldSOHAtLT WXfcMIWtO AMD »M rAMIWlAH WITH THI IN»C«WmTlON SUtWHTTtO 1H THIS ANO AVt ACCOMFANY1NQ OOCV)M6MT3. BASED
 3UIHY Of THOU INC1V10UALI IMMtOUTB.T MSPOMJUie C0« OiTAINI*iA«01 MAVf tllH OUCtOSCD. » THI lOXCS AflOVJ PO« QtNt^ATOW O« INOiPtNOCNT
 0* AH» CHCCKIO i ru«TMj* CI«TIFY THAT TH« WAITI CH»A*CT»WZATION UPON vwio* MINWA^ aintNOj COUP. WfLiis is ACCUWTE AWO COMPLETE.
 MAT Mt IMFLQYSH »NQ I MAT II HIUJ LIABtl UNOI« HQtH*», 1>W. ANQ THCLAWS Qt MANY STfTIl, K» 7W|I IMK*MAfl0N WOYlOfO IM THU »Q«U1 ANO
 MCANTIMO
                                                                                '/J

-------
 'M. OPRJNGS Corporation
 x500
 urtingtnn, Wl 53074
 M-9101
          03/24/92      09:39           MINERflL-SPRINGS CORP

                     WASTE STREAM PROFILE SHEET
                                                                                        008
                          (fa
                                                                              VT3I _ 10-2769
                                                                              HO*	
                                                                                   1J275
                                                                              OATE_3/ll/92_
                                                                              SAUSflEP
         MI6680090317
U.S.   E.P.A.,
                             Guardian
        24 Sixth St.
        Bay City,  MI   4B708
3BBUL CONTACT
                 Pmq Fro«hlich
'CONTACT	
 317 i.    894-2805
                 onr.xriori
Acidic
                       (R«d)
                       prep and
         Metal  Standard*
       Water
       Hydrochloric Acid
       Nitcic
                                                           U.S. E.P.A., Regxon 5
                                                 HlfCAOOflfSJ.
                                                           200 South  D«*rborn  Stro«t
                                                           ChicagoTTsoroe
                                                 CONTACT.
                                                                  janu» i Poromlo
                                             . 312  )_
                                         FA*  1      1.
                                         FOR CUSTOMER UM.
                                                               886-6587
3 Drums
                     Annually
                                                OltUfMt KVtHTDHY   1-2 Dfuma
                                                UaJDUVBWQ
                                                COLOR
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                                                QXDUEH
                                                        05 ooua
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                     D
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                                                                                 100
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                       < 1.5
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-------
      03/24x92      09:39
                                                   M1NERftL-SPRINGS  CORP
                                                                                   0Q9
                "RQ"  w*oto oxidi*«r,  Corroaive Liquid,  N.o.3.(Nitric  Acid, hydrochloric
                 (D001,odo2,b809)	'
:CLASS
HER
             Oxidizer
 NA919 J
'1/0.45T
                  0001,0002,0009
        Dm   fflno     OCXMMAAM           Dm  CSw      WATM fllAcnvE
        OYH   21 HO     nsncjccMcraRoet      Ons  tB HO      BUXOOOM.
                        JHoacstKimvt          Ovts  Quo
                                                                                           D^s
 •?"c.'.rc:j rj c
     C3TH   DMD
TTY DATA MMT (HKX) ATTX»«0
                                                          C.D
                                                              WOWMQ ON REHM/ VTX GSeWTCR
     TMAT I HAW *4ft«ONM.vr VXAMtNCO AMD AM FAMIIIAK WITH THC INTQAMATlON SUBMITTIO IN TMtS A NO ALL ACCOMPANV1NQ DOCUMENTS  BASED
my or THOSE INDIVIDUAL! iMMtoiAmr RUPCNSISL? ro« OBTAININO TH« iNfOHMATON. i ilUBVf THAT TH< SUBMITTED INITOWMATION is TBU£.
NO COMPifTI. ANO THAT ALL KMOWN OH  SUStCTtD MAlAflOJ HAVE 6UN DI8CL09IO  I* fH{ ROMS AfOVt 'C* QtNIKATOR OH INOEPENOEKT
« AMI CHiCKfO. I fUHTHCM CERTIFY THAT THE WAJTI CHAMCTE«liATION UPON WHICH MINIMAL yfllNOS C0«» RILII« IS ACCURATE ANO COMrLSTE-
«T MY IMPlOYtft ANO I MAY «| HCVO LIAflLI  UNOCfl HOIHM. LAW. ANO THg LAWS OP MANT *TATU. KW THI INFORMATION mOVIOIO IN TH»J ^O«M ANO
'ANVINO
                                                   TITL6

-------
                                      SECTION: APPENDIX R, ATTACHMENT 2
                                      VERSION: FINAL/MARCH 1997
                                      Attachment  2 - Cover
ATTACHMENT 2 - LABORATORY FLOOR PLAN
R/V LAKE GUARDIAN HAZARDOUS MATERIAL CONTINGENCY PLAN

-------

-------
                                                SECTION: APPENDIX R, ATTACHMENT 3
                                                VERSION: FINAL/MAY 1997
                                                PAGE:  1 of 4
Attachment 3 - CHEMICAL STORAGE BY ROOM
R7V LAKE GUARDIAN HAZARDOUS MATERIAL CONTINGENCY PLAN

* Indicates materials that may be stored in amounts equal to or greater than 1 gallon (4 liters).

Chemistry Lab

Acetic Acid (Glacial)
Acetone
Aerosol 22
l-Amino-2-Naphthyl-4-Sulfamic Acid
Ammonium Chloride
Ammonium Molybdate
Ammonium Peroxydisulfate (Ammonium Persulfate)
Antimony Potassium Tartrate
Anasorb Molecular Sieve
(L+) Ascorbic Acid
Barium Chloride
Buffer Salts (pH 4.01, pH 6.86, pH 9.18)
Bio Rex Sodium form Cation Exchange Resin
Brij-35
Cadmium (Coarse)
Cadmium Metal
Carbon (Activated)
Carbon Tetrachloride
Chelex 100
Corn Starch
Cupric Bromide
Cupric Bromide Powder
Cupric Sulfate 5 Hydrate
Disodium Ethylenediamine Tetraacetate
Drierite (CaSO4)
Ethanol
Ethylenediamine Dihydrochloride (Tetrasodium)
Ferric Nitrate 9 Hydrate
Ferrous Sulfate
Hexamethylenetetramine
Hydrochloric Acid
Hydrazine Sulfate
(L+) Glutamic Acid
Magnesium Carbonate
Magnesium Sulfate
Manganese Dioxide
Manganous Sulfate
Mercuric Oxide Red

-------
                                                 SECTION:  APPENDIX R, ATTACHMENT 3
                                                 VERSION:  FINAL/MAY 1997
                                                 PAGE:  2 of 4
Mercuric Thiocyanate
Mercury Indicator
Methanol
Methenamine
Methylmercuric Bromide
Methylmercury (II) Hydroxide
Methylthymol Blue
N-(l-Naphthyl) ethylenediamine Dihydrochloride
Nitric Acid
Oxalic Acid
Phenol
Phenolphthalein
(B) Phenylethylamine
Phosphoric Acid
Phosphorus Standard Solution
Platinizing Solution
Potassium Bi-iodate
Potassium Chloride
Potassium Dichromate
Potassium Hydroxide
Potassium Iodide
Potassium Nitrate
Potassium Persulfate
Potassium Phosphate Monobasic
Potassium Sulfate
Silicon Reference Solution
Soda Lime
Sodium Azide
Sodium Bicarbonate
Sodium Bisulfite
Sodium Borate
Sodium Bromide
Sodium Carbonate
Sodium Chloride
Sodium Citrate
Sodium Lauryl  Sulfte
Sodium Hydroxide
Sodium Meta-phosphate
Sodium Nitroprusside
Sodium Potassium Tartrate
Sodium Phosphate Dibasic
Sodium Silicate
Sodium Salicylate
Sodium Sulfate
Sodium Sulfite

-------
                                                SECTION:  APPENDIX R, ATTACHMENT 3
                                                VERSION:  FINAL/MAY 1997
                                                PAGE:  3 of 4
Sodium Thiosulfate
Stannous Chloride
Starch
Sulfanilamide
Sulfuric Acid
Biology Lab  (Starboard Lab)

Dricote
Glutaraldehyde
Irgalan Black Stain
Plate Count Agar
Wet Lab

Buffer Solutions pH 4, pH 7, pH 10
Club Soda
Formalin
Lugol's  Solution
Turbidity Standards 0.5 NTU, 10 NTU, 20 NTU
Extraction Lab*

Acetone
Hexane
Methanol
Methylene Chloride
Analytical Lab

Compressed Gases:    Helium
                     Nitrogen
                     Oxygen
                     5% Methane, 95% Argon
Primary Productivity

Ecoscint Scintillation Cocktail
Radiac Wash

-------
                                                SECTION:  APPENDIX R, ATTACHMENT 3
                                                VERSION:  FINAL/MAY 1997
                                                PAGE:  4 of 4
Hazardous Materials Locker*

Acetic Acid (Glacial)
Acetone
Ethanol
Formaldehyde
Hexane
Hydrochloric Acid
Lugol's Solution
Methanol
Methylene Chloride
Nitric Acid
Phosphoric Acid
Sulfuric Acid

-------
                                             SECTION:  APPENDIX R, ATTACHMENT 4
                                             VERSION:  FINAL/MAY 1997
                                             PAGE: 1 of 1
Attachment 4 - EMERGENCY PHONE NUMBERS
R/V LAKE GUARDIAN HAZARDOUS MATERIAL CONTINGENCY PLAN
In the event of a release of hazardous waste or hazardous materials, the Captain, with the assistance of
the  Emergency Coordinator, has the responsibility to  notify the GLNPO  office and appropriate
authorities such as the National Response Center and the  Coast Guard (Refer to Section IV.  A. of this
appendix).
FIRE DEPARTMENT	911 (Where Available)
POLICE DEPARTMENT	911 (Where Available)
DAVID ROCKWELL                                 (312)353-1373

A. JAMES FINN, SAFETY OFFICER                    (312) 353-2075

STEVE GURSKI (RSO)                               (312) 886-5301

AIR AND RADIATION DIVISION                      (312) 886-5301

NATIONAL RESPONSE CENTER                      (800) 424-8802

U.S. COAST GUARD
24 Hour Line - Great Lakes Region                      (800) 321-4400

U.S. COAST GUARD - Detroit                          (313) 568-9580

U.S. COAST GUARD - Toledo                          (419) 729-2651

MICHIGAN DNR                                     (800) 292-4706

OHIO EPA                                         (800) 282-9378

MICHIGAN STATE FIRE MARSHALL                  (517) 322-1924

OSHA                                              (800) 321-6742

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                                       SECTION: APPENDIX R, ATTACHMENT 5
                                       VERSION: FINAL/MARCH 1997
                                       Attachment 5 - Cover
ATTACHMENT 5 - EMERGENCY EQUIPMENT LOCATIONS

-------

-------
                                          SECTION: APPENDIX R, ATTACHMENT 6
                                          VERSION: FINAL/APRIL 1997
                                          PAGE:  1 of 1
Attachment 6 - EMERGENCY RESPONSE TEAM
EMERGENCY COORDINATOR
MELODY PIEPER,
EMERGENCY COORDINATOR	(517)894-2805 or (937) 435-9715

Or Dial 911 onboard the R/V Lake Guardian and report incident
ALTERNATE EMERGENCY COORDINATORS:


ALTERNATE EMERGENCY COORDINATOR	

Or Dial 911 onboard the R/V Lake Guardian and  report incident



ALTERNATE EMERGENCY COORDINATOR	

Or Dial 911 onboard the R/V Lake Guardian and  report incident

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                                                  SECTION:  APPENDIX R, ATTACHMENT 7
                                                  VERSION:  FINAL/MAY 1997
                                                  PAGE:  1 of 1
Attachment 7 - OSHA 29 CFR 1910.120
HEALTH & SAFETY PLAN COMPLIANCE PROCEDURES

I.      General

       The  following  "Generic Site Safety Plan" should be used in the event  that the U.S. EPA
       GLNPO R/V  LAKE GUARDIAN  Hazardous Waste/Hazardous Materials Contingency Plan
       becomes implemented.  Utilization of the plan will minimize response hazards and will assure
       compliance with applicable regulations.

II.     Respiratory Protection

       All Contingency Plan  Response Personnel, who enter exclusion  zones,  must utilize  SCBA
       (Level  "B")  respiratory  protection during  clean-up/response  efforts.    This  requirement
       eliminates the  necessity for Response Personnel to perform  and  determine the required levels
       of respiratory protection based on air monitoring  results.

       It is not anticipated that Level "A" personal protection will  be required for response activities
       onboard the R/V Lake Guardian.

III.    Air Monitoring Requirements

       Air monitoring for explosive atmospheres must be performed with a properly calibrated LEL
       meter when incidents involve unknown or confirmed flammable materials.  LEL  monitoring
       must be performed continuously during these types of response activities.

       The  GLNPO  R/V  Lake Guardian. Chemical  Hygiene Officer  is  responsible  for proper
       maintenance and calibration of the ship's LEL/O2 meter.

       During  response activities,  Response Personnel  should evacuate  any area where 10% LEL
       readings are observed in work areas and should  not resume operations until the readings fall
       below 10% LEL.  Readings of 10% are required  because of  "indoor" responses.

IV.    Decontamination

       Response  Personnel must  follow  guidelines  in  the  "Decontamination"  section  of  this
       attachment for  Level "B"  activities.  Materials for Level "B" decontamination  are provided
       onboard the R/V Lake Guardian..

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Appendix B.  Generic Site Safety Plan
This appendix  provides a generic  plan  based  on  a  plan  developed  by the
U.S. Coast Guard for  responding to  hazardous  chemical  releases.1  This
generic  plan can be adapted  for designing  a  Site  Safety  Plan  for hazardous
waste site cleanup operations.  It  is  not  all inclusive  and should only be
used as  a guide, not  a standard.
A.  SITE DESCRIPTION
    Date                            Location
    Hazards	
    Area affected
    Surrounding population_
    Topography	
    Weather conditions
    Additional information
B.  ENTRY OBJECTIVES - The objective of the initial entry to the contaminated
    area is to   (describes actions, tasks to be accomplished; i.e., identify
    contaminated soil; monitor conditions, etc.)	''	
C.  ONSITE ORGANIZATION AND COORDINATION - The following personnel are
    designated to carry out the stated job functions on site.   (Note: One
    person may carry out more than one job function.)
    PROJECT TEAM LEADER_
    SCIENTIFIC ADVISOR__
    SITE SAFETY OFFICER
    PUBLIC INFORMATION OPFICER_
    SECURITY OFFICER	
    RECORDKEEPER
    FINANCIAL OPPICER_
    FIELD TEAM LEADER_
    FIELD TEAM MEMBERS
^U.S.  Coast Guard.  Policy Guidance for Response to Hazardous Chemical
 Releases.   USCG Pollution Response COMDTINST-M16465.30.

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B-2      Appendix B
  FEDERAL AGENCY REPS  (i.e.,  EPA, NIOSH)
  STATE AGENCY REPS
 LOCAL  AGENCY  REPS
 CONTRACTORS)
 All personnel arriving or departing  the  site  should  log  in and out  with  the
 Recordkeeper.  All activities on site must  be cleared through the Project Team
 Leader.

 D.  ONSITE CONTROL

   (Name-of individual or agency	 has  been designated to coordinate
 access control and security on site.  A  safe  perimeter has been established
 at   (distance or description of controlled area)	
 No unauthorized person should be within this area.

 The onsite Command Post and staging area have been established at
 The prevailing wind conditions are 	.  This location is upwind
 from the Exclusion Zone.

 Control boundaries have been established, and the Exclusion Zone (the
 contaminated area), hotline, Contamination Reduction Zone, and Support Zone
 (clean area) have been identified and designated as follows:   (describe	
 boundaries and/or attach map of controlled area)	
 These boundaries are identified by:   (marking of zones, i.e., red boundary
 tape - hotline; traffic cones - Support Zone; etc.)	

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                                                                Appendix B
                                                                                B-3
E.  HAZARD  EVALUATION

The following  substance(s)  are  known or  suspected to be  on site.  The  primary
hazards of  each are  identified.
   Substances  Involved
   (chemical name)
                              Concentrations (If Known)
                    Primary Hazards

                 (e.g.,  toxic  c.i
                 inhalation ^
                                                         (i.e., slippery
The following additional hazards are expected on site:
ground, uneven terrain, etc.)	
Hazardous substance information form(s) for the involved substance(s) have
been completed and are attached.

F.  PERSONAL PROTECTIVE EQUIPMENT

Based on evaluation of potential hazards,  the following levels of personal
protection have been designated for the applicable work areas or tasks:
   Location
Exclusion Zone
                             Job Function
             Level of Protection
Contamination
Reduction Zone
A
A
A
A
A
A
A
A
B
B
B
B
B
B
B
B
C
C
C
C
C
C
C
C
D
D
D
D
D
D
D
D
Other
'-Bother
Other
Other
Other
Other
Other
Other
Specific protective equipment for each level of protection is as follows:

Level A
          Fully-encapsulating suit
          SCBA	
          (disposable coveralls)
Level C   Splash gear (type)	
          Full-face canister resp.
Level B   Splash gear (type)
          SCBA
                                           Level D
Other

-------
B-4      Appendix B
  The following protective clothing materials are  required  for  the  involved
  substances:

               Substance                                 Material

             (chemical name)	               (material name,  e.g., Viton)
  If air-purifying respirators are authorized,    (filtering medium)    is the
  appropriate canister for use with the involved substances and concentrations.
  A competent individual has determined thai, all criteria for using this type of
  respiratory protection have been met.

  NO CHANGES TO THE SPECIFIED LEVELS OF PROTECTION SHALL BE MADE WITHOUT THE
  APPROVAL OF THE SITE SAFETY OFFICER AND .HE PROJECT TEAM LEADER.

  G.   ONSITE WORK PLANS

  Work party(s)  consisting of 	 persons  will  perform the following tasks:

     Project Team Leader     (name)	     	(function)	
    Work  Party
    Work  Party  12
    Rescue  Team
      (required for
      entries  to  IDLH
      environments)
    Decontamination
     Team
 The work party(s) were briefed on  the contents of  this  plan  at

-------
                                                               Appendix B      B-5
H.  COMMUNICATION PROCEDURES

Channel 	 has been designated as  the  radio  frequency  for  personnel  in the
Exclusion zone.  All other onsite communications will use channel 	.

Personnel in the Exclusion Zone should remain  in constant radio  communication
or within sight of the Project Team  Leader.  Any failure of  radio
communication requires an evaluation of whether personnel should leave the
Exclusion zone.

  (Horn blast, siren, etc.)	 is the emergency signal  to indicate  that all
personnel should leave the Exclusion Zone.  In addition, a loud  hailer is
available if required.

The following standard hand signals will be used in case of failure  of radio
communications:

    Hand gripping throat 	 Out of air, can't breathe
    Grip partner's wrist or 	 Leave area immediately
      both hands around waist
    Hands on top of head	Need assistance
    Thumbs up	OK, I am all right,  I understand
    Thumbs down	No, negative

Telephone communication to the Command Post should be established as soon as
practicable.  The phone number is	.

I.  DECONTAMINATION PROCEDURES                                       ^_

Personnel and equipment leaving the Exclusion Zone shall be thoroughly
decontaminated.  The standard level 	 decontamination protocol shall be
used with the following decontamination stations:   (1)  	
(2)  	 (3) 	 (4)	(5) 	
(6)  	 (7) 	 (8)	(9) 	
(10) 	 	 Other	
Emergency decontamination will include the following stations:
The following decontamination equipment is required:
  (Normally detergent and water)	 will be used as the decontamination
solution.

J.  SITE SAFETY AND HEALTH PLAN

1.  	(name)	 is the designated site Safety Officer and is
directly responsible to the Project Team Leader for safety recommendations on
site.

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B-6      Appendix B
  2.  Emergency Medical Care

        (names of qualified personnel)	 are the qualified EMTs on site.
        (medical facility names)	  , at   (address)	
      phone 	 is located 	 minutes from this location.
        (name of person)	was contacted at   (time)   and briefed on
      the situation, the potential hazards, and the substances involved.   A map
      of alternative routes to this facility is available at   (normally  Command
      Post)	.

      Local ambulance service is available from  _  	at
      phone 	.   Their response time is 	 minutes.
      Whenever possible, arrangements should be made for onsite  standby.

      Piret-aid equipment is available on site at the following  locations:
           First-aid kit
           Emergency eye wash
           Emergency shower
             (other)
      Emergency medical information for substances  present:

      Substance                Exposure symptoms          First-Aid  Instructions
      List of emergency phone  numbers:

      Agency/Facility          Phone  I                   Contact
      Police	
      Fire     _________________________________________
      Hospital	
      Airport  	
      Public Health Advisor
  3.   Environmental  Monitoring

      The following  environmental  monitoring  instruments shall be used on site
      (cross out if  not  applicable)  at  the  specified  intervals.

      Combustible Gas  Indicator  -  continuous/hourly/daily/other 	
      02  Moaitor                -  continuoua/hourly/daily/other 	
      Colorimetric Tubes        -  continuous/hourly/daily/other 	
          (type)	      	
      HNU/OVA                   -  continuous/hourly/daily/other
      Other  	    -  continuous/hourly/daily/other
      	    -  continuous/hourly/daily/other

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                                                               Appendix B      B-7
4.   Emergency Procedures (should be modified as required for incident)

    The following standard emergency procedures will be used by onsite
    personnel.   The Site Safety Officer shall be notified of any onsite
    emergencies and be responsible for ensuring that the appropriate
    procedures  are followed.

    Personnel Injury in the Exclusion Zone:  Upon notification of an injury in
    the Exclusion Zone, the designated emergency signal 	
    shall  be sounded.   All  site  personnel  shall  assemble at the
    decontamination line.   The rescue  team will  enter  the Exclusion Zone (if
    required)  to  remove the injured  person to  the hotline.   The Site Safety
    Officer  and Project Team Leader  should evaluate  the  nature of the injury,
    and  the  affected  person should be  decontaminated to  the extent possible
    prior  to movement  to the Support Zone.   The  onsite EMT  shall initiate the
    appropriate first  aid,  and contact  should  be made  for an ambulance and
    with the designated medical  facility  (if required).   No persons shall
    reenter  the Exclusion Zone until the cause of the  injury or symptoms is
    determined.

    Personnel  Injury  in the Support  Zone:  Upon notification of an injury in
    the Support Zone,  the Project Team  Leader  and Site Safety Officer will
    assess the nature  of the injury.   If the cause of  the injury or loss of
    the injured person does not  affect  the  performance of site personnel,
    operations may  continue,  with the onsite EMT initiating the appropriate
    first aid and necessary follow-up as stated  above.   If  the injury
    increases the risk  to others, the designated  emergency  signal
   	  shall be  sounded and  all  site  personnel  shaH. move
    to the decontamination  line  for  further instructions.   Activities on site
   will stop until the  added  risk is removed  or  minimized.

    Fire/Explosion: Upon notification of a  fire  or explosion  on site,  the
   designated emergency signal	shall be  sounded  and
   all site personnel  assembled at  the decontamination  line.   The  fire
   department shall be  alerted and  all personnel  moved  to  a  safe distance
   from the involved  area.

   Personal Protective  Equipment Failure:  If  any  site worker  experiences  a
   failure or alteration of  protective equipment  that affects the  protection
   factor,  that person  and his/her  buddy  shall  immediately leave the
    Exclusion Zone.  Reentry  shall not  be  permitted  until the  equipment  has
   been repaired or replaced.

   Other Equipment Failure:  If any  other  equipment  on site fails to  operate
    properly, the Project Team Leader and  Site Safety  Officer  shall be
   notified and then  determine the  effect  of  this failure  on  continuing
   operations on site.  If  the failure affects  the  safety  of  personnel  or
   prevents completion  of  the Work  Plan tasks,  all  personnel  shall leave  the
    Exclusion Zone  until the  situation  is evaluated  and  appropriate actions
   taken.

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B-8      Appendix B
      The following emergency escape  routes are designated  for  use  in  those
      situations where egress from the Exclusion Zone cannot occur  through
      the decontamination line:   (describe alternate routes to  leave  area in
      emergencies)	
      In all situations, when an onsite emergency results in evacuation of the
      Exclusion Zone, personnel shall not reenter until:

         1.  The conditions resulting in the emergency have been corrected.
         2.  The hazards have been reassessed.
         3.  The Site Safety Plan has been reviewed.
         4.  Site personnel have been briefed on any changes in the Site Safety
             Plan.

  5.   Personal Monitoring

      The following personal monitoring will be in effect on site:

      Personal exposure sampling:     (describe any personal sampling programs
      being carried out on site personnel.   This would include  use of sampling
      pumps,  air monitors, etc.)	
      Medical monitoring:  The expected air  temperature will be   (   °F)      If
      it is determined that heat  stress monitoring is required  (mandatory  if
      over 70°F) the following procedures shall be followed:
        (describe procedures in effect,  i.e.,  monitoring body temperature,  body
      weight, pulse rate)            	
  All  site  personnel  have  read  the  above  plan  and  are  familiar  with  its
  provisions.

  Site  Safety  Oficer  	( name)	  	(signature)
  Project Team Leader	
  Other  Site Personnel                                            	

-------
Appendix C.  Sample Hazardous Substance Information Form
COMMON NAME:
CHEMICAL NAME:
I. PHYSICAL/CHEMICAL PROPERTIES

   Natural physical  state:  Gas	
   (at ambient temps of  20°C-25°C)
   Molecular weight
   Density3
   Specific gravity3
   Solubility: water
   Solubility5: 	
   Boiling point
   Melting point
   Vapor  pressure
   Vapor  density
   Flash  point
     (open cup	;  closed cup	
   Other:
II. HAZARDOUS CHARACTERISTICS

 A. TOXICOLOGICAL HAZARD  HAZARD?

    Inhalation            Yes   No
    Ingestion             Yes   No
    Skin/eye absorption    Yes   No
    Skin/eye contact       Yes   No
    Carcinogenic          Yes   No
    Teratogenic           Yes   No
    Mutagenic             Yes   No
    Aquatic               Yes   No
    Other:  	     Yes   No

 B. TOXICOLOGICAL HAZARD  HAZARD?
    Combustibility        Yes   No
    Toxic byproduct(s):    Yes   No
                                                                   SOURCE
                                    Liquid
               Solid
g/g-mole
g/ml
e
§
*
°F/°C
°F/°C
°F/°C
°F/°C
°F/°C
mmHg @
e
°F/°C
°F/°C
°F/°C
J
       CONCENTRATIONS
      (PEL,  TLV, other)
       CONCENTRATIONS
                                                                 SOURCE
                                                                 SOURCE
    Flammability          Yes   No
      LFL                          	    	
      UFL	    	
    Explosivity           Yes   No
      LEL                          	    	
      UEL	    	

30nly one is necessary.
DFoc organic compounds,  recovery of  spilled  material  by ssolvent  extraction  may
 require solubility data.

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C-2
Appendix C
    C. REACTIVITY HAZARD
       Reactivities:
    D. CORROSIVITY HAZARD
       Neutralizing agent:
    E.  RADIOACTIVE HAZARD
       Background
       Alpha particles
       Beta particles
       Gamma radiation
                    HAZARD?
                    Yes  No
                    HAZARD?
                    Yes  No
                    HAZARD?
                    Yes  No
                    Yes  No
                    Yes  No
                    Yes  No
  III.  DESCRIPTION OP INCIDENT:
       Quantity involved 	
       Release information
                                         CONCENTRATIONS
                          SOURCE
CONCENTRATIONS
SOURCE
EXPOSURE RATE
SOURCE
       Monitoring/sampling  recommended
   IV.  RECOMMENDED PROTECTION:

       Worker              	
       Public
    V.  RECOMMENDED SITE CONTROL:

       Hotline          	
       Decontamination  line
       Command  Post  location
   VI.  REFERENCES  FOR SOURCES:

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                                                                Appendix C
                                                                                 C-3
     SAMPLE HAZARDOUS SUBSTANCE INFORMATION FORM FILLED OUT  FOR VINYL CHLORIDE
COMMON NAME:
                                 CHEMICAL NAME:
                                              :  C ^ )
I. PHYSICAL/CHEMICAL PROPERTIES

   Natural physical state: Gas ^  _ Liquid
   (at ambient temps of 20°C-25°C)
   Molecular weight                	
   Density3                        	
   Specific gravity3
   Solubility: water
   Solubility13:   e* \CQ^ •>!
   Boiling point
   Melting point
                                                Solid
                                      o.im   §   1.0
                                                       _g/g-mol__
                                                        g/ml
                                                  —    "F/0C
                                                 	_°F/°C
                                            7-.T-
                                          -7.MH. 8"
                                                         FJ°C
    Vapor pressure                   l^oo mmHg  (§_
    Vapor density                       1-1-  Jj
    Flash point                     	~ II O
      (open cup__v^__; closed cup	)
    Other: P»Jy*»i *«•;**/ ro•**
      UPL                           	33  	     	
    Explosivity           Yes  No
      LEL                           	   -        	
      UEL	

30nly one is necessary.
t>For organic compounds, recovery of spilled material by solvent extraction may
 require solubility data.

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C-4
     Appendix C
C. REACTIVITY HAZARD

   Reactivities :
               /\ air,
            i'i
                             HAZARD?
                            ''YeT) No
                             HAZARD?
                             Yes ^No""
D. CORROSIVICf HAZARD
       Neutralizing agent:
       RADIOACTIVE HAZARD
       BacKground
       Alpha particles
       Beta particles
       Gamma radiation
  III.  DESCRIPTION OF INCIDENT:
       Quantity  involved  	A_
       Release  information
                                     CONCENTRATIONS
                                                                   SOURCE
CONCENTRATIONS
                                                               SOURCE
                                     EXPOSURE RATE
                          SOURCE
       Monitoring/sampling  recommended
   IV.  RECOMMENDED  PROTECTION:
       Worker _ Level &
                                          or  (/ ,'
       Public
   V.  RECOMMENDED  SITE  CONTROL:

       Hotline
       Decontamination  line
       Command  Post  location
  VI. REFERENCES FOR SOURCES:
                 —  Oil
                                                                 ; A Is .
        o$HA-  -2^  CFtZ.  fart-  tlio./ol?-

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 Appendix D.  Sample Decontamination Procedures for
               Three Typical Levels of Protection3
 F.S.O.P.  No. 7

 Process:   DECONTAMINATION PROCEDURES



 INTRODUCTION
     1.1   The objective of these procedures is to minimize the risk of
          exposure to hazardous substances.  These procedures were derived
          from the U.S. Environmental Protection Agency, Office of
          Emergency and Remedial  Response's (OERR), "Interim Standard
          Operating Safety Guides (revised Sep.  82)".   This version of the
          guides is in a format that is more appropriate for use in the
          field.

     1.2   Protective equipment must be worn by personnel when response
          activities involve  known or suspected  hazardous substances.   The
          procedures for decontaminating personnel  upon leaving the
          contaminated area are addressed for each  of  the EPA,  OERR
          designated levels of protection.   The  procedures given are for
          the maximum and minimum amount of decontamination used for each
          level  of protection.

     1.3   The maximum decontamination procedures for all  levels of
          protection consist  of specific activities at nineteen stations.
          Each station emphasizes an important aspect  of decontamination.
          When establishing a  decontamination line, each aspect should be
          incorporated separately or combined with  other aspects into  a
          procedure  with fewer steps (such  as the Minimum Decontamination
          Procedures).

     1.4   Decontamination lines are  site specific since they are dependent
          upon the  types of contamination and the type of work  activities
          on  site.   A cooling  station is sometimes  necessary within the
          decontamination line  during hot weather.  It is usually a
          location  in a  shaded area  in which  the wind  can help  to cool
          personnel.   In addition,  site conditions may permit the use  of
          cooling  devices such  as cool  water  hose,  ice packs, cool  towels,
          etc.   When the decontamination line is no longer required,
          contaminated wash and rinse solutions  and contaminated articles
          must be  contained and disposed of as hazardous  wastes in
          compliance with state and  federal  regulations.
a Source: Excerpted from Field Standard Operating Procedures for the Decon-
       tamination of Response Personnel (FSOP 71. EPA Office of Emergency
       and Remedial Response, Hazardous Response Support Division,
       Washington, DC. January 1985.

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D-2
Appendix D
    P.S.O.P. No.  7


    PROCESS  DECON PROCEDURES
                               MAXIMUM DECONTAMINATION LAYOUT


                                      LEVEL A  PROTECTION
1

Outer
Remc
q
f
j
EXCLUSION i
ZONE
Boot Cover
Glove Tape &
>val Removal Glove Wash
Boot Cover Boot Cover &
Removal Glove Rinse u/.
r
r
Segregated
J Equipment
Drop
-ri IMC

              Tank Change <^T*	
              and Redress • Boot Cover/
             -r          Outer Gloves
                                    7 j  Suit/Safety Boot
                                            Wash
                              Suit/Safety Boot
                                 Rinse
               CONTAMINATION
                  REDUCTION
                     ZONE
                                   fjO]  Safety Boot
                                         Removal

                                   fJTl  Fully Encapsulating Suit
                                        and Hard Hat Removal
                        (l2j  SCBA Backpack
                               Remova!
                                   (^3)   Inner Glove
                                         Wash
                                   ^4]   Inner Glove
                                         Rinse
                                   [15]   Face Piece
                                         Removal
                                   [^5]   Inner Glove
                                         Removal
                                   [17]   Inner Clothing
                                        Removal
                              Field
                              Wash
                                 -Wl9] Redress
                                                               CONTAMINATION
                                                               'CONTROL LINE   '
                                                          SUPPORT
                                                            ZONE

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                                                                    Appendix D
                                                   D-3
P.S.O.P.  No.  7


PROCESS  DECON  PROCEDURES
                           MAXIMUM  DECONTAMINATION LAYOUT

                                 LEVEL B PROTECTION
                           Outer Glove
                            Removal
EXCLUSION
   ZONE

       Tape
     Removal
                   Boot Cover
                       &
                   Glove Wash
                                    Boot Cover
                                     Removal
             Boot Cover 8:
             Glove Rinse
                                                                     Segregated
                                                                     Equipment
                                                                       Drop
         Tank Change
         and Redress • Boot Cover/
                   Outer Gloves
                               7 J   Suit/Safety Boot
                                        Wash
Suit/SCBA/Boot/Glove
     Rinse
                              ho]
Safety Boot
 Removal
                                                               • HOTLINE
          CONTAMINATION
           REDUCTION
              ZONE
                              [11]
                              [13]
SCBA Backpack
  Removal
Splash Suit
 Removal


Inner Glove
 Wash
                                   Inner Glove
                                     Rinse

                                   Face Piece
                                    Removal
                                   Inner Glove
                                    Removal
                                   Inner Clothing
                                    Removal
                         Field
                         Wash
   -#{l9) Redress
                                                          CONTAMINATION
                                                         'CONTROL LINE
                                                      SUPPORT
                                                        ZONE

-------
D-4
Appendix D
F.S.O.P. No.  7


PROCESS  DECON PROCEDURES
                           MAXIMUM DECONTAMINATION  LAYOUT


                                  LEVEL C  PROTECTION

Canister or s
Mask Change L^
EXCLUSION
Z°NE Boot Cover
Outer Glove Tape &
Removal Removal Glove Wash
k T Boot Cover Boot Cover &
Removal Glove Rinse
r
Segregated
v Equipment
J Drop
(V) Suit/Safety Boot
| Wash
S " ^-^ Rinco

            and Redress - Boot Cover/
                      Outer Gloves
                                        Safety Boot
                                         Removal
             CONTAMINATION
               REDUCTION
                  ZONE
                             Field
                             Wash
                              Splash Suit
                              Removal


                              Inner Glove
                               Wash


                              Inner Glove
                               Rinse


                              Face Piece
                               Removal


                              Inner Glove
                               Removal

                              Inner Clothing
                              Removal
                                      Redress
                                                              CONTAMINATION
                                                              1 CONTROL LINE
                                                         SUPPORT
                                                           ZONE

-------
                                                                   Appendix D
D-b
F.S.O.P. No.  7

PROCESS  DECON  PROCEDURES
                         MINIMUM DECONTAMINATION LAYOUT

                             LEVELS A & B PROTECTION
                                                               WIND DIRECTION
                              Redress: Boot Covers
                              and Outer Gloves
uj Decon
— Solution
o 6vl&
1

s^
Decon Outer ^r
Equipment Garments/
Dr°P 	 ^ >X Remove
.X^ Boot Covers
>X and Outer Gloves
\^->. UJ 1 \-^
D !• O
Plastic O 1 Can

SPt^ '

Water phTar
Chang*
^^^^ ""»•
t

k
>-Over
Point






Sheet | (10 gallon)





O ^
V *

Remove
Boots/Gloves
,
Outer
Garments
(For Disposal
and Off Site
Decontamination)
b
Can
(32 gallon)


^ •
REMOVE
SCBA

-------
D-6
Appendix D
P.S.O.P.  No.  7

PROCESS   DBCON PROCEDURES^
                          MINIMUM DECONTAMINATION LAYOUT

                                LEVEL C  PROTECTION
                                                                 WIND DIRECTION
                               I  Redress: Boot Covers
                               I  and Outer Gloves
LU I Decon
— | Solution
1 1
Decon Outer s'
Equipment Garments ; /
Drop —> / Remove
.S Boot Covers
x'and Outer Gloves
20°
©
Water Cartridge or Canister
Change-Over
Point
» k ftft »-
* ' W *
b \\ b
Plastic O 1 Can
Sheet I (10 gallon)


^^^^
Remove
Boots/Gloves
and
Outer
Garments
(For Disposal ^
and Off Site
Decontamination)
b
Can
(32 gallon)
i '
REMOVE
MASK

-------
                                                                                 Appendix D
                                           D-7
 EQUIPMENT NEEDED TO  PERFORM  MAXIMUM DECONTAMINATION  MEASURES FOR LEVELS A,  B,  AND  C
   Station 1:  a. Various Size  Containers
              b. Plastic Liners
              c. Plastic Drop  Cloths

   Station 2:  a. Containers (20-30 Gallons)
              b. Oecon Solution or Detergent Water
              c. 2-3 Long-Handled, Soft-Bristled
                 Scrub Brushes

   Station 3:  a. Containers (20-30 Gallons)
                            OR
                 High-Pressure Spray Unit
              b. Water
              c. 2-3 Long-Handled, Soft-Bristled
                 Scrub Brushes

   Station 4:  a. Containers (20-30 Gallons)
              b. Plastic Liners

   Station 5:  a. Containers (20-30 Gallons)
              b. Plastic Liners
              c. Bench or Stools

   Station 6:  a. Containers (20-30 Gallons)
              b. Plastic Liners

   Station 7:  a. Containers (20-30 Gallons)
              b. Decon Solution or Detergent Water
              c. 2-3 Long-Handled, Soft-Bristled
                 Scrub Brushes

   Station 8:  a. Containers (20-30 Gallons)
                            OR
                 High-Pressure Spray Unit
              b. Water
              c. 2-3 Long-Handled, Soft-Bristled
                 Scrub Brushes

   Station 9:  a. A1r Tanks or  Face Masks and
                 Cartridge Depending on Level
              b. Tape
              c. Boot Covers
              d. Gloves
Station 10: a.  Containers  (20-30 Gallons)
            b.  Plastic  Liners
            c.  Bench  or Stools
            d.  Boot Jack

Station 11: a.  Rack
            b.  Drop Cloths
            c.  Bench  c- Stool-

Station 12: a.  Table

Station 13: a.  Basin  or Bucket
            b.  Decon  Solution
            c.  Small  Table

Station 14: a.  Water
            b.  Basin  or Bucket
            c.  Small  Table

Station IS: a.  Containers (20-30 Gallons)
            a.  Plastic  Liners

Station 16:  a.  Containers (20-30 Gallons)
            S>.  Plastic  Liners

Station 17:  a.  Containers (20-30 Gallons)
            15.  Plastic  Liners

Station 18:  a.  Water
            s.  Soap
            c.  Small  Table
            d.  Basin  or Bucket,- .
            e.  Field  Showers
            f.  Towels

Station 19:  a.  Dressing Trailer 1s  Needed 1n
               Inclement Weather
            b.  Tables
            c.  Chairs
            d.  Lockers
            e.  Cloths
EQUIPMENT NEEDED TO  PERFORM MINIMUM DECONTAMINATION MEASURES FOR LEVELS A,  B,  AND  C
  Station 1:  a.  Various  Size Containers
              b.  Plastic  Liners
              c.  Plastic  Drop Cloths

  Station 2:  a.  Containers  (20-30 Gallons)
              b.  Decon  Solution
              c.  Rinse  Water
              d.  2-3 Long-Handled, Soft-Bristled
                 Scrub  Brushes

  Station 3:  a.  Containers  (20-30 Gallons)
              b.  Plastic  Liners
              c.  Bench  or Stools
Station 4:   a.  A1r Tanks or Masks and
               Cartridges Depending Upon Level
            b.  Tape
            c.  Boot Covers
            d.  Gloves

Station 5:   a.  Containers (20-30 Gallons)
            b.  Plastic Liners
            :.  Bench or Stool s

Station 6:   a.  Plastic Sheets
            b.  Basin or Bucket
            c.  Soap and Towels
            d.  Bench or Stools

Station 7:   a.  Water
            b.  Soap
            c.  Tables
            d.  Wash Basin or Bucket

-------
D-8
Appendix D
                   FSOP 7:   MAXIMUM MEASURES  FOR LEVEL  A DECONTAMINATION
           Station  1:
            Segregated  Equipment
            Drop
Station
Station
Station
Station
Station
Station
Statfon
Station
2:
3:
4:
5:
6:
7:
8:
9:
Soot Cover and
Glove Wash
Boot Cover and
Glove Rinse
Tape Reooval
Boot Cover
Removal
Outer Glove
Removal
Suit and Boot
Wash
Suit and Boot
Tank Change
          Station 10:


          Station 11:





          Station 12:



          Station 13:


          Station 14:


          Station 15:


          Station  16:
           Safety Boot
           Removal

           Fully Encapsulating
           Suit and Hard Hat
           Removal
           SCBA Backpack
           Removal
           Inner Glove Wash
           Inner Glove Rinse
           Face Piece  Removal
           Inner  Glove
           Removal
  1  Deposit equipment used on site  (tools,  sampling
     devices and containers,  monitoring  Instruments,
     radios, clipboards,  etc.) on  plastic  drop cloths
     or 1n different containers with  plastic  liners.
     During hot weather operations, a cool down
     station may be set up  within  this area.

  2. Scrub outer boot covers  and gloves  with  decon
     solution or detergent/water.

  3. Rinse off decon solution from station 2  using
     copi • is amounts of water.

  4. Remove tape around boots and  gloves and  deposit
     in container with  plastic  liner.

  5. Remr-'e boot covers and deposit in container
     with  plastic liner.

  6. Remove outer gloves and  deposit  in container
     with  plastic Uner.

  7. Wash  encapsulating suit  and boots using  scrub
     brush and  decon  solution or detergent/water.
     Repeat as  many  times as  necessary.

  8. Rinse off  decon  solution using water.   Repeat as
     many  times  as necessary.

  9.  If an air  tank change 1s desired, this is the
     last  step  in the decontamination  procedure.
     Air tank is  exchanged,  new outer  gloves  and  boot
     covers  donned,  and joints taped.   Worker returns
     to duty.

10.  Remove  safety boots and deposit  in container
     with  plastic Uner.

11.  Fully encapsulated suit Is removed with
     assistance of a helper  and laid out  on a  drop
     cloth or hung up.  Hard hat is removed.   Hot
     weather rest station  maybe set up within  this
     area  for personnel  returning to site.

12.  While still wearing facepiece, remove  backpack
     and place on table.  Disconnect hose from
     regulator valve and proceed to next  station.

13.  Wash with decon solution  that  will not harm  the
     skin.   Repeat as often  as necessary.
14. Rinse with water.
    necessary.
Repeat as many times as
15. Remove face piece.   Deposit  in container with
    plastic liner.   Avoid  touching face with fingers.

16. Remove inner  gloves  and deposit in container
    with liner.

-------
                                                                      Appendix D
                                                        D-9
        FSOP  7:   MAXIMUM  MEASURES FOR LEVEL  A DECONTAMINATION
 Station 17:   Inner Clothing
             Removal
Station 18:  Field Wash
Station 19:  Redress
17. Remove clothing  and  place  1n lined container.
    Do not wear Inner  clothing off-site since there
    1s a possibility that  small amounts of
    contaminants might have been transferred 1n
    removing the fully-encapsulating suit.

18. Show-.-r 1f highly toxic, sk1n-corrc:1ve or skln-
    absorbable materials are known or suspected to
    be present.  Wash  hands and face 1f shower 1s
    not available.

19. Put on clean clothes.
        FSOP  7:   MINIMUM  MEASURES FOR  LEVEL A DECONTAMINATION
Station  1:  Equipment  Drop
Station  2:  Outer Garment,
             Boots,  and Gloves
             Wash and Rinse
Station  3:  Outer  Boot and
             Glove  Removal

Station  4:  Tank Change
Station  5:   Boot,  Gloves
             and Outer Garment
             Removal

Station  6:   SC8A Removal
Station  7:   Field Wash
 1.  Deposit equipment used on-slte  (tools,  sampling
    devices and containers, monitoring  Instruments,
    radios, clipboards,  etc.)  on plastic  drop
    cloths.  Segregation at the drop  reduces the
    probability of cross contamination.   During hot
    weather operations,  cool down stations  maybe set
    up within this area.

 2.  Scrub outer boots,  outer gloves and fully-
    encapsulating suit  with decon solution  or
    detergent and water.  Rinse off using copious
    amounts of water.

 3.  Remove outer boots  and gloves.  Deposit 1n
    container with plastic Uner.

 4.  If worker leaves  Exclusion  Zone to change air
    tank, this Is the last step 1n the
    decontamination procedure.   Worker's  air tank is
    exchanged, new outer gloves and boot  covers
    donned, joints taped,  and worker  returns to duty.

 5.  Boots, fully-encapsulating  suit,  Inner  gloves
    removed and deposited 1n separate containers
    lined with plastic.

 6.  SCBA backpack and facepiece Is removed  (avoid
    touching face with  fingers).  SCBA deposited
    on plastic sheets.
7. Hands and face  are  thoroughly washed.
   soon as possible.
Shower as

-------
D-10
Appendix D
                   FSOP 7:   MAXIMUM MEASURES FOR LEVEL  B DECONTAMINATION
           Station  1:
           Segregated Equipment
           Drop
Station
Station
Station
Station
Station
Station
2:
3:
4:
5:
6:
7:
Boot Cover and
Glove Wash
Boot Cover and
Glove Rinse
Tape Removal
Boot Cover
Removal
Outer Glove
removal
Suit and Safety
Boot Wash
          Station  8:


          Station  9:
           Suit,  SCBA, Boot,
           and  Glove R1n^e

           Tank  Change
          Station 10:


          Station 11:



          Station 12:


          Station 13:

          Station 14:

          Station 15:
           Safety Boot
           Removal

           SCBA  Backpack
           Removal
           Splash  Suit
           Removal

           Inner Glove Wash

           Inner Glove Rinse

           Face Piece Removal
  1.  Deposit  equipment used on site (tools, sampling
     devices  and containers, monitoring Instruments,
     radios,  clipboards, etc.) on plastic drop
     cloths or 1n different containers with plastic
     liners.  Segregation at the drop reduces the
     probability of cross-contamination.   During hot
     weather  operations, cool down stations may be set
     up within this area.

  2.  Scrub outer boot covers and gloves with decon
     solution or detergent and water.

  3.  Rinse off decon solution  fro* station 2 using
    copious amounts of water.

  4. Remove tape around boots  and gloves  and deposit
     In container with plastic  Uner.

  5. Remove boot covers and  deposit 1n  container
    with plastic Uner.

  6. Remove outer gloves  and deposit 1n container
    with plastic Uner.

  7. Wash chemical-resistant splash  suit,  SCBA,
    gloves and  safety boots.   Scrub with  long-handle
    scrub brush and decon solution.  Wrap  SCBA
    regulator (1f  belt mounted  type) with  plastic to
    keep out  water.   Wash backpack  assembly with
    sponges or  cloths.

 8. Rinse off decon  solution using copious amounts
    of water.

 9. If worker leaves  exclusion zone to change air
    tank, this  1s  the last  step  1n  the
    decontamination  procedure.   Worker's air tank Is
    exchanged,  new  outer gloves  and boot covers
    donned, and  joints taped.  Worker  returns to
    duty.

10. Remove safety boots and deposit In container
    with plastic 1 Iner.

11. While still  wearing  faceplece,  remove  back-
    pack and  place  on table.  Disconnect hose from
    regulator valve.

12. With assistance  of helper, remove  splash suit.
    Deposit 1n  container with plastic  liner.

13. Wash Inner  gloves with  decon solution.

14. Rinse Inner  gloves with water.

15. Remove face  piece.  Deposit  1n container with
    plastic liner.   Avoid touching  face with fingers,
          Station 16:   Inner Glove
                        Removal
                                 16. Remove inner gloves  and  deposit In container
                                     with Uner.

-------
                                                                     Appendix D
                                                       D-11
         FSOP  7:   MAXIMUM MEASURES  FOR  LEVEL B  DECONTAMINATION.
Station 17:   Inner  Clothing
             Removal
Station 18:   Field Wash
Station 19:   Redress
17. Remove Inner  clothing.   Place 1n container with
    Hner.  Do  not wear  Inner clothing off-site
    since there 1s a possibility that small amounts
    of contaminants might have been transferred 1n
    removing the  fully-encapsulating suit.

18. Shower 1f highly toxic, skin-corrosive or skln-
    absorbable  materials are known or suspected to
    be present.   Hash hands and face If shower 1s
    not available.

19. Put on clean  clothes.
        FSOP  7:   MINIMUM  MEASURES FOR LEVEL  B DECONTAMINATION
Station  1:  Equipment Drop
Station  2:   Outer  Garment,
             Boots,  and Gloves
             Wash and  Rinse
Station  3:   Outer  Boot and
             Glove  Removal

Station  4:   Tank Change
Station  5:   Boot,  Gloves
             and Outer Garment
             Removal

Station  6:   SCBA Removal
Station  7:   Field Wash
 1. Deposit equipment  used  on-s1te (tools, sampling
    devices and  containers, monitoring Instruments,
    radios, clipboards, etc.) on plastic drop
    cloths.  Segregation at the drop reduces the
    probability  of  cross contamination.  During hot
    weather operations, cool down station may be set
    up within  this  area.

 2. Scrub  outer  boots, outer gloves and chemical-
    resistant  splash suit with decon solution or
    deteryent  water.   Rinse off using copious
    amounts of water.

 3. Remove outer boots and gloves.   Deposit 1n
    container  with  plastic Hner.

 4. If worker  leaves exclusive zone to change air
    tank,  this Is the  last step In  the
    decontamination procedure.   Worker's  air tank 1s
    exchanged, new  outer gloves and boot  covers
    donned,  Joints  taped, and worker returns to  duty.

 5. Boots,  chemical-resistant splash suit,  Inner
    gloves  removed  and deposited In separate
    containers lined with plastic.

 6.  SCBA backpack and faceplece Is  removed.   Avoid
    touching face with finger.   SCBA  deposited
    on plastic sheets.
 7.  Hands and face are thoroughly  washed.
    soon as possible.
                                                                             Shower as

-------
D-12
Appendix  D
                   FSOP  7:  MAXIMUM  MEASURES FOR  LEVEL C  DECONTAMINATION
            Station  1:  Segrated Equipment
                        Drop
           Station  2:  Boot Cover and
                        Glove Wash

           Station  3:  Boot Cover and
                        Glove Rinse

           Station  4:  Tape Removal
           Station  5:  Boot Cover
                        Removal

           Station  6:  Outer Glove
                        Removal

           Station  7:  Suit and Boot
                        Wash
           Station  8:   Suit and  Boot.
                        and Glove Rinse

           Station  9:   Canister  or
                        Mask Change
           Station  10:   Safety Boot
                        Removal

           Station  11:   Splash Suit
                        Removal

           Station  12:   Inner Glove
                        Rinse

           Station  13:   Inner Glove
                        Wash

           Station  14:   Face Piece
                        Removal

           Station  15:   Inner Glove
                        Removal
                                   1. Deposit equipment used on site (tools, sampling
                                      devices and containers, monitoring instruments,
                                      radios, clipboards, etc.) on plastic drop
                                      cloths or 1n different containers with plastix
                                      liners.  Segregation at the drop reduces the
                                      probability of cross contamination.   During hot
                                      weather operations, a cool  down station may be
                                      set up within this area.

                                   2. Scrub outer boot covers and gloves with decon
                                      solution or detergent and water.

                                   3. Riiise off decon solution  from  station  2 using
                                      copious amounts of water.

                                   4. Remove tape around boots  and gloves  and  deposit
                                      in container with plastic liner.

                                   5. Remove boot covers and  deposit  1n  containers
                                      with plastic 1 iner.

                                   6. Remove outer gloves  and deposit  in container
                                      with plastic 1 iner.

                                   7. Wash splash  suit,  gloves, and safety boots.
                                      Scrub with  long-handle  scrub brush and  decon
                                      solution.

                                   8. Rinse off decon  solution using water.  Repeat  as
                                      many times  as  necessary.

                                   9.  If worker leaves exclusion zone to change
                                      canister (or mask), this is the last step
                                      in the  decontamination procedure.  Worker's
                                      canister is exchanged, new outer gloves and boot
                                      covers  donned, and joints taped worker returns
                                      to duty.

                                  10.  Remove  safety boots and deposit in container
                                      with  plastic 1 iner.

                                  11.  With assistance of helper, remove splash
                                      suit.   Deposit in container  with plastic liner.

                                  12.  Wash  inner gloves with decon solution.
                                 13. Rinse in^er gloves with water.
                                 14. Remove face piece.  Deposit in container  with
                                     plastic liner.  Avoid touching face  with  fingers.

                                 15. Remove inner gloves and deposit in lined
                                     container.

-------
                                                                     Appendix  D
                                                       D-13
        FSOP 7:   MAXIMUM MEASURES  FOR  LEVEL C  DECONTAMINATION
Station 16:   Inner Clothing
             Removal
Station 17:   Field Wash
Station 18:   Rediess
16. Remove clothing  soaked with perspiration and
    place In lined container.  Do not wear Inner
    clothing off-site since there Is a possibility
    that small  amounts of contaminants might have
    been transferred in removing the fully-
    encapsulating suit.

17. Shower 1f highly toxic, skin-corrosive or skin-
    absorbable  materials are known or suspected to
    be present.  Wash hands and face if shower 1s
    not available.

18. Put on clean clothes.
        FSOP  7:   MINIMUM  MEASURES FOR LEVEL  C DECONTAMINATION
Station  1:   Equipment  Drop
Station  2:   Outer Garment,
             Boots,  and Gloves
             Wash and  Rinse

Station  3:   Outer Boot and
             Glove Removal

Station  4:   Canister  or
             Mask Change
Station  5:  Boot,  Gloves
             and Outer  Garment
             Removal

Station  6:  Face Piece
             Removal
 1. Deposit  equipment used on-slte (tools, sampling
    devices  and  containers, monitoring Instruments,
    radios,  clipboards, etc.) on plastic dr6f>
    cloths.   Segregation at the drop reduces the
    probability  of cross contamination.   During hot
    weather  operations, a cool down station may be
    set up within this area.

 2. Scrub outer  boots, outer gloves and  splaih
    suit with decon solution or detergent water.
    Rinse off using copious amounts of water.

 3. Remove outer boots and gloves.  Deposit in
    container with plastic Hner.

 4. If worker leaves exclusive zone to change
    canister (or mask), this is the last step in the
    decontamination procedure.  Worker's canister is
    exchanged, new outer gloves and boot covers
    donned,  Joints taped, and worker returns to duty.

 5. Boots, chemical-resistant splash suit, inner
    gloves removed and deposited in separate
    containers lined with plastic.

 6. Facepiece is removed.  Avoid touching face with
    fingers,  Facepiece deposited on plastic sheet.
Station  7:  Field Wash
 7. Hands and  face  are  thoroughly washed.
    soon as  possible.
                                                                             Shower as

-------
                                       SECTION: APPENDIX R, ATTACHMENT
                                       VERSION: FINAL/MARCH 1997
                                       Attachment 8 - Cover
ATTACHMENT 8 - NOTIFICATION TO EMERGENCY ASSISTANCE AUTHORITIES

-------
                                               SECTION: APPENDIX  S
                                               VERSION: FINAL/MARCH 1997
                                               PAGE 1 of 13
APPENDIX S:      GLNPO RESEARCH VESSELS-PROCEDURES FOR THE
TRANSPORTATION OF HAZARDOUS MATERIALS /WASTE BY GREAT LAKES
NATIONAL PROGRAM OFFICE VIA GOVERNMENT VEHICLE AND GOVERNMENT
DRIVER
TABLE OF CONTENTS
                                                           Page

I.     Packaging                                             2

II.    Bill of Lading                                          2

III.    Labeling                                              5

IV.    Placarding                                             5

V.    Loading and Unloading                                  6

VI.    Flammable Liquids                                      8

VII.   Flammable Solids and Oxidizing Materials                   8

VIII.  Corrosive Materials                                     8

IX.    Carbon-14                                             9

X.    Hazardous Materials Incidents                             11

XI.    Hazardous Substance Discharge Notification                  13

XII.   Hazardous Waste                                       13


Attachment One:          Sample US Government Bill of Lading

Attachment Two:          DOT Form F 5800.1

Attachment Three:         Sample Uniform Hazardous Waste Manifest

-------
                                                    SECTION:  APPENDIX S
                                                    VERSION:  FINAL/MARCH 1997
                                                    PAGE 2 of 13
GLNPO  RESEARCH  VESSELS:    PROCEDURES  FOR  THE  TRANSPORTATION  OF
HAZARDOUS MATERIALS/WASTE BY GREAT LAKES NATIONAL PROGRAM OFFICE VIA
GOVERNMENT VEHICLE AND GOVERNMENT DRIVER
It is the policy of the United States Environmental Protection Agency, Region 5, to comply with all
applicable regulations when transporting hazardous materials by government vehicle. This document
discusses the Department of Transportation (DOT) regulations for transporting hazardous materials.
Great Lakes National Program Office will follow the regulations as required.  All materials/waste
must be transported by government vehicle and government employee driver based upon provisions
set forth by the carrier.
I.      PACKAGING

When transporting hazardous materials, the materials should be in the original  containers and
shipping cartons/crates as received from the supplier.  If this is not feasible,  Great Lakes will
package the materials in DOT-approved packaging materials prior to shipment.  It is important to
note that cardboard packaging must be protected from wetting.  If a cardboard package loses its
structural integrity for any reason, the packaging is not longer DOT approved. Therefore, tarpaulins
or covered vehicles should be used when transporting such packages.
II.     BILL OF LADING

A bill of lading must be used for each shipment.  Refer to Attachment 1 of this Appendix for
sample US Government Bill of Lading.  The following items must be completed for each bill of
lading:

       A.    Origin
             •      From Milwaukee:

                    United States Environmental Protection Agency
                    Great Lakes National Program Office
                    c/o University of Wisconsin
                    Great Lakes Research Program
                    1600 E. Greenfield Avenue
                    Milwaukee, WI

             •      From Chicago:

                    United States Environmental Protection Agency
                    Great Lakes National Program Office

-------
                                              SECTION: APPENDIX  S
                                              VERSION: FINAL/MARCH 1997
                                              PAGE 3 of 13
             536 S. Clark
             Chicago, IL 60604

             From Bay City:

             United States Environmental Protection Agency
             Great Lakes National Program Office
             R/V Lake Guardian
             24 Sixth Street
             Bay City, MI 48708
B.     Shipper
             United States Environmental Protection Agency
             Great Lakes National Program Office
             230 S. Dearborn
             Chicago, IL 60604
C.     Destination/Consignee

       • To Milwaukee:
             United States Environmental Protection Agency
             Great Lakes National Program Office
             c/o University of Wisconsin
             Great Lakes Research Program
             E. Greenfield Avenue
             Milwaukee, WI
        To Bay City:
             R/V Lake Guardian
             Warehouse
             102 Ninth Street
             Bay City, MI  48708

-------
                                                SECTION:  APPENDIX S
                                                VERSION:  FINAL/MARCH 1997
                                                PAGE 4 of 13
D.     Transportation Company:

              United States Environmental Protection Agency

E.     Place an X in the column marked HM before each hazardous material listed on the
       bill of lading.

F.     Identify the hazardous material using the proper DOT shipping name, hazard class,
       identification number, and reportable  quantity,  if applicable. Refer  to  49 CFR
       172.101 for a Hazardous Materials Table in regards to transportation.

G.     List the total quantity or weight of the hazardous material.

H.     Write/type the following statement on the bill  of lading:

       "This is to certify that the above-named materials are properly classified, described,
       packaged,  marked, and labeled, and  are  in proper  condition for transportation
       according to the applicable regulations of the DOT."

       Copies of the completed bill of lading shall be forwarded to the Region 5 Health and      ^
       Safety Office, the GLNPO office, and a file maintained on board the Lake Guardian.

       A driver of a vehicle containing hazardous material shall ensure that the bill of lading
       is readily available to, and recognizable by, authorities in the event of accident or
       inspection.  Specifically, the driver shall:

       1.     Clearly distinguish the bill of lading, if  it is carried  with other shipping
              papers or other papers of any kind, by  either distinctively tabbing it or by
              having it appear first; and

       2.     Store the shipping paper as follows:

              a.      When the driver is at the vehicle's  controls, the shipping paper shall
                     be:

                     1)     Within his/her immediate reach while he/she is restrained by
                            the lap belt; and

                     2)     Either  readily  visible to  a person entering the  driver's
                            compartment or in a holder which is mounted to the inside of
                            the door  on the driver's side of the  vehicle.                     ^^

-------
                                                      SECTION: APPENDIX S
                                                      VERSION: FINAL/MARCH 1997
                                                      PAGE 5 of 13
                     b.      When the driver is not at the vehicle's controls, the shipping paper
                            shall  be:

                            1)     In a holder which is mounted to the inside of the door on the
                                  driver's side of the vehicle; or

                            2)     On the driver's seat in the vehicle.
III. LABELING

Each package must be marked as required by 49 CFR, Part 172, Hazardous Materials Table.  For
example, acetone packages must be labeled with a red diamond-shaped flammable label.
IV. PLACARDING (49 CFR  Part 172.503)

If the government vehicle is transporting the following materials, placards (markings on the sides
of the vehicle) are required:

              •      Class A explosives
                    Class B explosives
              •      Poison A
              •      Flammable solid ("Dangerous When Wet" label only)
              •      Radioactive material
                    Uranium hexafluoride, fissile (containing more than  1.0 percent U235)
              •      Uranium hexafluoride, low specific activity (containing 1.0 percent or less
                    U235)

       A placard is NOT required when transporting 60 millicuries or less of Carbon-14.

       If the government  vehicle is transporting less than 1000 pounds total of the following
       materials, placards  (markings on the side of the vehicle) are NOT required:

                    Class C explosives
              •      Blasting agents
              •      Nonflammable gas (chlorine)
              •      Nonflammable gas (oxygen, cryogenic liquid)
              •      Flammable gas
                    Combustible liquid
                    Flammable solid
              •      Oxidizer
              •      Organic  peroxide

-------
                                                       SECTION:  APPENDIX  S
                                                       VERSION:  FINAL/MARCH 1997
                                                       PAGE 6 of 13
              •       Poison B
              •       Corrosive material
              •       Irritating material

       For example, if the vehicle contains 600 Ibs. of phenol (a poison B material), it would not
       be placarded.  But, if the truck contained 600 Ibs. of phenol and 400 Ibs.  of acetone (a
       flammable liquid), a  placard is necessary.  In this instance, since two types of hazardous
       materials are being transported, a ":Dangerous" placard would be used. Refer to 49 CFR
       Part 172, Hazardous  Materials Table, when determining the type of placard required.
V.  LOADING AND UNLOADING

       The following requirements will be  adhered  to when loading or unloading hazardous
       materials:

              A.     Any tank, barrel, drum,  cylinder or other packaging, not permanently attached
                    to a motor vehicle, which contains any flammable liquid, compressed gas,
                    corrosive material,  poisonous material,  or radioactive material  must be
                    secured against movement within the vehicle on which it is being transported,
                    under conditions normally incident to transportation. For example, crates or
                    cartons of acetone must be strapped via a method that prevents movement of
                    the package.

              B.     Smoking on or about any motor vehicle while loading or unloading any
                    explosive,  flammable  liquid,  flammable  solid, oxidizing  material or
                    flammable compressed gas is forbidden.

              C.     During the loading or unloading of any explosive,  flammable  liquid,
                    flammable solid oxidizing material or flammable compressed gas into or from
                    any motor vehicle extreme care shall be taken to  keep fire away  and to
                    prevent persons in the vicinity from smoking, lighting matches, or carrying
                    any flame or lighted cigar, pipe or cigarette.

              D.    No hazardous material shall be loaded into or on,  or unloaded from, any
                    motor vehicle unless the handbrake be securely set and all other reasonable
                    precautions be taken to prevent motion  of the motor vehicle during such
                    loading or unloading procedures.

              E.    No tools which are  likely to damage the effectiveness of the closure of any
                    package or other container, or  likely  adversely to  affect such package or
                    container, shall  be  used for the loading or unloading of any explosive or
                    other dangerous  article.

-------
                                                      SECTION:  APPENDIX  S
                                                      VERSION:  FINAL/MARCH 1997
                                                      PAGE 7 of 13
              F.      Containers of explosives, flammable liquids, flammable solids, oxidizing
                     materials, corrosive materials, compressed gases, and poisonous liquids or
                     gases, must be so braced as to prevent motion thereof relative to the vehicle
                     while in transit. Containers having valves or other fittings must be so loaded
                     such that there will be the minimum likelihood of damage thereto during
                     transportation.

              G.      Reasonable care should be taken to prevent  undue rise in temperature  of
                     containers and their contents during transit. There must be no tampering with
                     such container or the contents thereof, nor any discharge of the contents of
                     any container between point of origin and point of  billed  destination.
                     Discharge of contents of any container must not be made prior to removal
                     from the motor vehicle.

              H.      In any single vehicle, hazardous materials shall not be  loaded together if
                     prohibited  by the  loading and storage  chart (Refer to  49 CFR 177.848,
                     Segregation and Separation Chart of Hazardous Materials.)
VI. FLAMMABLE LIQUIDS

       Unless the engine of the motor vehicle is to be used for the operation of a pump,  no
       flammable liquid shall be loaded into, or on, or unloaded from any motor vehicle while the
       engine is running.

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                                                       SECTION:  APPENDIX S
                                                       VERSION:  FINAL/MARCH 1997
                                                       PAGE 8 of 13
VII.  FLAMMABLE SOLIDS AND OXIDIZING MATERIALS

       When transporting flammable solids and oxidizing materials, the following shall DC adhered
       to:

       A.     All flammable solids and oxidizing materials shall be contained entirely within the
              body of the vehicle and shall be covered by suitable means, and if the vehicle has
              a tailgate, it shall be closed and secured in place during transportation.

       B.     Special care shall be taken in the loading of any motor vehicle with flammable solids
              or oxidizing materials which are likely to become hazardous to transport when wet,
              to keep them  from  being wetted during the loading process and to keep them dry
              dunng transit. For example, care should be taken with ground, crushed or pulverized
              charcoal, and lump charcoal.

       C.     Whenever a motor carrier  has knowledge concerning the hazards of spontaneous
              combustion or heating of any material to be loaded on a motor vehicle, the material
              shall be loaded to afford sufficient ventilation of the load to provide reasonable
              assurance against fire; and the motor vehicle shall be unloaded as soon as practicable
              after reaching its destination.
VIII.  CORROSIVE MATERIALS

       A corrosive material is a liquid or solid that causes visible destruction or irreversible
       alterations in human skin tissue at the site of contact, or in the case of leakage from its
       packaging, a liquid that has a severe corrosion rate on steel.

       The following discusses the general requirements for transportation of corrosive materials.

       •      Bottles containing corrosive liquids must have enough headspace to allow for heat
              expansion.  The package  must not be completely  full of liquid at 130 degrees
              Fahrenheit.

       Bottles containing corrosive liquids may not be packed in the same package with any other
       hazardous materials, except:

              Bottles containing corrosive liquids cushioned by  noncombustible, nonreactive,
              absorbent material and securely packed in  tightly closed  metal  packaging, except
              hydrofluoric acid, which must be overpacked in packaging other than one made of
              metal, may be packed with other hazardous materials. This exception does not apply
              to the following chemicals:

-------
                                                       SECTION:  APPENDIX S
                                                       VERSION:  FINAL/MARCH 1997
                                                       PAGE 9 of  13
                     nitric acid exceeding 40 percent concentration, perchloric acid, hydrogen
                     peroxide exceeding 52 percent strength by weight, or nitrodydrochloric or
                     nitrohydrochloric 
-------
                                                SECTION:  APPENDIX  S
                                                VERSION:  FINAL/MARCH 1997
                                                PAGE 10 of 13
       •      Although not required by DOT regulations, a bill of lading will be completed
              for  each shipment of limited  quantities of radioactive materials.   The
              radioactive material will be identified on the bill of lading as Excepted
              Radioactive Material, limited quantity,  n.o.s., UN2910  When  shipping
              Carbon-14-contaminated water to Central Regional Laboratory, the laboratory
              will be  given  a copy of the  bill  of lading  in addition to the normal
              distribution list.

       •      Instead of the shipper's certification statement, the following notice will be
              placed on the bill of lading (this notice IS required by DOT regulations):

                    "This package conforms to the conditions and limitations specified in
                    49 CFR 173.421 for excepted radioactive material, limited quantity,
                    n.o.s., UN2910."

When transporting Carbon-14  in solid sodium bicarbonate form, the material should be in
the original packaging as received by GLNPO; and the packaged material must be placed in
a cooler that has been filled with some type of cushioning material to prevent breakage of
the ampules.  The cooler should be secured via straps or some other method to  prevent
movement during transport.

When transporting Carbon-14-contaminated water, the carboys must be secured via straps,
tarpaulin or some other appropriate method to prevent movement of the carboys during
transport.

-------
                                                      SECTION: APPENDIX S
                                                      VERSION: FINAL/MARCH 1997
                                                      PAGE 11 of 13
X.  HAZARDOUS MATERIALS INCIDENTS

       In the event of ANY incident involving release of hazardous materials during the course of
       transportation (including loading, unloading, and temporary storage), the Region 5 safety
       officer must be  immediately notified.

       Immediate notice to the Department of Transportation (DOT), and Coast Guard Command
       Center/National Response Division in Washington, D.C., of hazardous materials incidents
       must be given if, as a direct result of hazardous materials:

              •      A person is killed,

              •      A person receives injuries requiring hospitalization,

              •      Estimated carrier or other property damage exceeds $50,000,

              •      Fire,  breakage,  spillage  or  suspected  radioactive  contamination occurs
                    involving shipment of radioactive material,

              •      Fire, breakage, spillage or suspected contamination of etiologic agents; or,

              •      A situation exists that, in the judgment of the carrier, should be reported, e.g.
                    a continuing  danger to life exists at the scene of the incident.

       Each notice to the DOT, as required above,  shall be given to the  DOT National Response
       Division, Washington, D.C., by telephone (800-424-8802).  Notice involving etiologic agents
       may be given to  the Director, Center for Disease Control Prevention, Emergency Response
       Branch 24-Hour Hotline (404-633-5313), in place of the notice to the DOT.  Each notice
       must include the following information:

                    Name of reporter

              •      Name and address of carrier  represented by reporter

                    Phone number where reporter can be contacted

              •      Date, time, and location of incident

                    Classification, name, and quantity of hazardous materials involved, if such
                    information is available

              •      Type of incident, nature of  hazardous  material involved, and whether a
                    continuing  danger to life exists at the scene.

-------
                                                     SECTION: APPENDIX  S
                                                     VERSION-. FINAL/MARCH 1997
                                                     PAGE 12 of 13
       In addition, each reportable incident shall be reported, in writing, in duplicate, on DOT Form
       F 5800.1 to the DOT within 15 days of the date of discovery.  Also, unintentional releases
       of hazardous materials from a package must be reported using DOT Form F 5800.1, see
       Attachment 2 of this Appendix.  The report shall be sent to:

                    Information Systems Manager
                    Research and Special  Programs Administration
                    Department of Transportation
                    Washington, D.C.  20590

       The above oral and written reporting requirements  do NOT apply to:

             •      Consumer commodities

             •      Paint or paint-related materials when shipped in packages of five gallons or
                    less

             •      Wet electric storage battery, either filled with acid or alkali.


XI. HAZARDOUS SUBSTANCE DISCHARGE NOTIFICATION

       When a hazardous substance is discharged in a REPORTABLE QUANTITY into or upon
       the navigable waters or adjoining shorelines, the person in charge of the vessel, transport
       vehicle,  or facility  shall  notify the U.S.  Coast Guard  National Response Center
       (800-424-8802), and furnish to the official to whom the discharge notification is made:

             •      The information required in Form F 5800.1, Section  VIII

             •      The name of the shipper of the hazardous substance

             •      The quantity of the hazardous substance, if known.

       An estimate of the quantity of the hazardous substance removed from the scene and the
       manner of disposition of any unremoved hazardous substance shall be entered in Part H of
       Form F5800.1.  To determine the reportable quantity for a spilled chemical, one should
       consult the Hazardous Materials Table (for example, a spill involving 1000 or more pounds
       of phenol is a reportable spill).  Refer to 49 CFR 177.848, Segregation and Separation Chart
       of Hazardous Materials.

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                                                     SECTION: APPENDIX S
                                                     VERSION: FINAL/MARCH 1997
                                                     PAGE 13 of 13
XII.  HAZARDOUS WASTE

       When transporting RCRA/TSCA waste  materials  from GLNPO  Research Vessels to
       secondary storage areas via government vehicle and government employee driver, GLNPO
       must comply with the regulations applicable to the transportation of those materials. EPA's
       regulations  incorporate  and require compliance with the DOT provisions  on labeling,
       marking,  placarding, using proper containers and reporting discharges.

       When transporting RCRA/TSCA waste  materials  from GLNPO Research  Vessels, the
       following guidelines must be followed:

             •      Manifest

                    The waste materials must be manifested (See Attachments of this appendix
                    for  sample Uniform Hazardous Waste  Manifest)  and  the designated
                    government driver must keep the manifest with the hazardous waste.  After
                    successful transport of the waste materials, the driver must return a copy of
                    the  manifest to the Chemical Hygiene Officer or her/his designate.  If the
                    driver is not able to deliver the waste to the designated storage area, he is to
                    return the waste to the GLNPO Research Vessel.

             •      Containers/Labeling

                    Waste containers of materials to be transported must be labeled in accordance
                    with RCRA/TSCA regulations prior to transport. Labels should include the
                    waste type, date of accumulation, RCRA waste code number, TSCA label if
                    greater than 50 ppm, and the EPA Hazardous Waste Generator I.D. Number.
                    Each waste container (5-gallon plastic carboy provided by waste contractor)
                    should be transported within a secondary containment tray or pan, and the
                    lids should  be secured prior to transport.  Wastes must not be mixed or
                    combined prior to transport.

             •      Emergency  Response

                    In the  event  of a discharge of waste  materials, the driver must  take
                    immediate action to protect human health and the environment, including
                    treatment or containment of the spill  and notification of proper authorities.
                    During the time of transport, the driver must possess (and be formally trained
                    in  the  use  of)  the  GLNPO  Hazardous Materials/Hazardous  Waste
                    Contingency  Plan.  Clean-up  practices  indicated in the plan should be
                    followed  if necessary.  Adequate spill supplies should accompany waste
                    materials during transport. The driver must also comply with Sections X and
                    XI of this document in the event of an incident involving hazardous waste.

-------
                                        SECTION: APPENDIX S, ATTACHMENT 1
                                        VERSION. FINAL/MARCH 1997
                                        Attachment 1 - Cover
ATTACHMENT 1 - BILL OF LADING
                    H3C
                    oar

-------
U.S. GOVERNMENT BILL OF LADING
1 TRANSPORTATION COMPANY itNDEREDTO
5 DESTINATION (Name, address and ZIP code)
9 CONSIGNEE (Name, address and ZIP code ol installation)
2 SCAC
6 SPLC (Dest)
7. SPLC (Ong.)
10 GBLOC (Cons)
ORIGINAL jjg>o- 0031888
3. DATE B/L PREPARED 4 ROUTE ORDER/RELEASE NUMBER
8 ORIGIN (Name, address and ZIP code)
1
11. SHIPPER (Name, address and ZIP code)
' 2. APPROPRIATION CHARGEABi E 1 3. BILL CHARGES TO (DepL/agency. bureau/office mailing address and ZIP codt
14 VIA (Route shipment when advantageous to the Government)
AGENCY LOC CODE
 15. MARKS AND ANNOTATIONS (II extra services are ordered, see Administrative Directions No. 2 on reverse)
16 PACKAGES
  NO   KINO
               18. DESCRIPTION OF ARTICLES (Use earner's classification or tanfl description it possible.
                  otherwise use a clear nontechnical description)
                                                            19. WEIGHT'
                                                              (Pounds only)
                                                                     FOR USE OF BILLING CARRIER ONLY
                                                                                                        Services
                                                                                                                    Rate
                                                                                                                                Charges
                 CLASSIFICATION ITEM NO
                                                                                                       TOTAL
                                                                                                       CHARGES
20 TARIFF/SPECIAL RATE AUTHORITY
                     21. PICKUP SERV. FURNISHED  (~| YES

                         VEHICLE FULLY LOADED  Q YES
                                                                                 SHIPPER'S
                                                                                 INITIALS
                                                                                         22. CARRIER WAY/FREIGHT BILL NO. AND DATE
23. STOP THIS SHIPMENT AT
     FOR:
                             24. FURNISH INFORMATION ON CAR/TRUCKLOAD/CONTAINER SHIPMENTS
                                 INITIALS & NO.
                                                          SEAL NUMBERS
                                                   APPLIED BY
                                                                                     LENGTH/CUBE
                                                                                  ORDERED
                                                                                             FURNISHED
                                                                                                         MARKED CAPACITY
                                                                                                        ORDERED   FURNISHED
                                                                                                                            DATE FURNISHED
 25. CARRIER'S PICKUP DATE
    (Year, month. A day)
                                 26a. SIGNATURE OF AGENT
                                                                                26b. PER
                                                                                                       B/L NUMBER
                                                                                                       D-  0,031,888
27. MODE
            28. ESTIMATE
29 NO. OF
 CLS/TLS
30. TYPE
 RATE
                                               31. PSC
                                                       32. REASON
f leceivec. by the transportation company named above, subject to conditions named on
the reverse hereof, the property hereinafter described, in apparent good order and
condition (contents and value unknown), to be forwarded to destination  by the said
company and connecting lines, there to be delivered in like good order and  condition to
said consignee.	
                      FOR USE OF ISSUING OFFICE
                                                                        CERTIFICATE OF CARRIER BILLING—CONSIGNEE MUST NOT PAY ANY CHARGES
33a ISSUING OFFICE (Name and complete address)
                                             34a. DELIVERED ON
                                                  (Year, month & day)
    'SSUING OFFICER
                                                         33b. GBLOC
                                                                                                       34b. AT (Actual delivery point)
                                                                       34c. BY (Name ot delivering earner)
                                             34d. DELIVERED THIS CONSIGNMENT COMPLETE 4 IN APPARENT GOOD ORD|
                                                  EXCEPT AS MAY BE INO.CATED    ,-,
33d CONTRACT/PURCHASE ORDER NO. OR OTHER
    AUTHORITY
                                                         33e. DATED
331 FOB POINT NAMED IN CONTRACT
                                             348
                                              D
                                              D
                                              D
                                       CARRIER OS4O REPORT
                                       ATTACHED
                                       DELIVERY AT DESTINATION
                                       FURNISHED
                                       ACCESSORIAL SERVICES
                                       CERTIFICATION ATTACHED
                                                                                                       341. NAME OF BILLING CARRIER
                                                                                                       34g. SIGNATURE OF CARRIER'S AGENT

-------
                                        SECTION: APPENDIX S, ATTACHMENT 2
                                        VERSION: FINAL/MARCH 1997
                                        Attachment 2 - Cover
ATTACHMENT 2 - DOT Form F 5800.

-------
      08-20-S3  11:HAM   FROM 202  366  1435             TO  85134393995             P002
                                               DEPARTMENT OF TRANSPORTATION
                                            HAZARDOUS MATERIALS INCIDENT REPORT

    REQUIREMENTS: The regulation! requiring reporting of hazardous material! Incldantt are contained in the Code of Federal Regulations (CFR), Tltlo 49
    Peru 100 to 179 (governing the transport of hazardous materiali by rail, air, water and highway). Failure to comply with the reporting requirement* contain
    ihmVm can rotuli In a civil penalty.

    A Guide for Preparing ths Hazardous Material; Incident Report it available from the Information Syttems Manager, Office of Hazardous Matarlalt
    Transportation, DHM-63, Research and Special Pronrems Administration. U.S. Department of TrnntpnrtAtlnn, WMhlngtnn, fin ?asan

    PUBLIC RfiPORTlNG BURDEN FOR THIS COLLECTION OF INFORMATION IS ESTIMATED TO AVERAGE 1 HOUR  PER RESPONSE, INCLUDING
    THE TIME FOR REVIEWING INSTRUCTIONS, SEARCHING EXISTING DATA SOURCES. GATHERING AND MAINTAINING THE DATA NEEDED,
    AND COMPLETING AND HCVICWING THE COLLECTION OP INFORMATION.  SEND COMMENTS REGARDING t HIS BUHUtN tSTIMA 11 OH AN
    OTHER ASPECT OF THIS COLLECTION OF INFORMATION, INCLUDING SUGGESTIONS I'Oft REDUCING THIS BURDEN, TO INFORMATION
    SYSTEMS MANAGER, OFFICE OF HAZARDOUS MATERIALS TRANSPORTATION. DMH-63, RESEARCH AND SPECIAL PROGRAMS
    ADMINISTRATION. U.S. DEPARTMENT OF TRANSPORTATION. WASHINGTON. DC 20590: AND TO THE OFFICE OF INFORMATION AND
     1EGULATORY AFFAIRS. OFFICE OF MANAGEMENT AND BUDGET. WASHINGTON, DC 20503.                                   ^



                                                             \R H|R£	 ._^.a^,Jf^.	.  „.	
03

-------
                                             HAZARDOUS MATERIALS INCIDENT REPORT
                                                                                                    FOfrTl AQorOVflO w "^ a ^u *.
                 Submi
                                 in duplicate to th< Informat.on Svswni Monager, O«ic« of Hazordous Material. Transporwtion. DHM-63. Resa.rch an
                                 ~->rtment of Tmnjportatior,, Wajhington, O.C  20590. If ip** provided for any itsm « .nodaquati. compl*-g that
                           	,.,„ , numb«r beinfl completed. Copie? ot this torm. m nm.Md qu«ntW9S, may b« obtnineU from ih« Infoimwion SYJH
                 Hazardous Materials Tran«portation, Additional copies In this pre^cribod format m»Y b« reproducod ond used, .f on th« csmo fl« arxJ km
  if papor.
                                          j ,
      MODS, DATE. AND LOCATION OF INCIDENT"
    MODE OF TRANSPORATION
                                D  AIR
                                                  G HIGHWAY
                                                                         RAIL
                                U WATER
                                                                                                              OTHER
|s.  DATE AND TIME OF INCIDENT
      (Use Military TimO, O.fl. 8:30am = 0830,
      noon = 1200, 6pm = 1800, mldnignt = 2400)
                                                                              /
                                                                                                              TIME: ..	
 3.  LOCATION OP INCIDENT (Includo airport 
-------
VII. PACKAGING INFORMATION: If tha package Is ovsrpack«d (consist* of sovero packagsj, a.g glass jars within a fiberoourd box}. Dealn vvitn Column f
for Information on tho Innarmott package.
ITEM
30. TYPE OF PACKAGING, INCLUDING INNER
, RECEPTACLES (a.q. Steel drum, tank car)
I3l1 CAPACITY OR WEIGHT PER UNIT PACKAGE
(e.g. 55 gallons, 65 Iba.)
UMBER OF PACKAGES OF SAME TYPE WHICH
•AILED IN IDENTICAL MANNCD
33. NUMBER OF PACKAGES OF SAME TYPE IN
SHIPMENT
34. PACKAGE SPECIFICATION IDENTIFICATION
{a.g. DOT 176, DOT W5AW UN U1 or none)
35 ANY OTHER PACKAGING MARKINGS
(e.g. STC. 18/16-5^88, Y1 4/150/87)
36. NAME AND ADDRESS. SYMBOL OR REGISTRATION
NUMBER OF PACKAGING MANUFACTURER
37. SERIAL NUMBER OF CYLINOEBJ, PORTABLE TANKS,
CARGO TANKS, TANK CARS
38. TYPE OF LABELING OR PLACARDING APPLIED
39.
IF RECONDITIONED
OR REOUALIFIED
A. REGlSTFiATION
NUMBER OR SYMBOL
0. OAT6 OP LAST
TEST OR INSPECT ION
40. EXEMPTION/APPROVAL/COMPETENT AUTHORITY
NUMBER, IF APPLICABLE (« fl. DOT E1012)
A B












c

M
™










VIII. DESCRIPTION OF PACKAGING FAILURE: Chock all applicable box«s for trie packaga(s) idaniltiaa above.
41. ACTION CONTRIBUTING TO PACKAGING FAILURE
A 1 Q. A 1 C.
a. D CD D TRANSPORT VEHICLE COLLISION |. D Q CD CUHHuSlON
b. D G D TRANSPORT VEHICLE OVERTURN K. D n n METAL FATIGUE
c. D Q Q OVERLOADING/OVERFILLING i. G G D FRICTION/RUBBING
d I'l D D LOOSE FITTINGS, VALVES m. Q P D FIRE/HEAT
e. G G D DEFECTIVE FITTINGS, VALVES n G G D FREEZING
1. D G D DROPPED o. Q Q D VENTING
fl, Q Q D STRUCK/RAMMED p. D D Q VANDALISM
-. D D D IMPROPER LOADING q. D D D INCOMPATIBLE MATERIALS
. D G D IMPROPER BLOCKING f. D D D OTHER 	 	 „
•V HOW PACKAGE(S) FAILED 44, PACKAGE AREA THAT FAILED
A. S. Q. A 3. S.
D CD D PUNCTURED a. Q Q D END, FORWARD
b, D D D CRACKED b. D D D END, REAR
C. D Q D BURST/INTERNAL PRESSURE «. D Q C] SIDE, RIGHT
d D Q D HIPPED d D D D SIDE, LEFT
e Q D D CRUSHED e. D D D TOP
1. n d O RUBBED/ABRADED f. U Q Q BOTTOM
0 D D G RUPTURED 0- G U U CENTER
h n n n mx™ n n n n n-mpp


42, OBJECT CAUSING FAILURE
A B C
a. D LJ O OTHER FREIGHT
b D D (I FORKLIFT
C H G G NAIL/PROTRUSION
d G [1 Q OTHER TRANSPORT VEH
c U G U WATER/OTHER LIQUID
1. U L~l D GROUND/FLOOR/ROADWJ
g. D G D ROADSIDE OBSTACLE
h. n n D NONE
1. D U D OTHER __
45 WHAT FAIL
A U
a. d n
b. D G
=. G D
d. n a
«, a a
i. a a
g. D G
h D a
ED ON PACKAGED) JM
- V
Cl BASIC PACKAGE MATM
D FITTING/VALVE
P CLOSURE
Q CHIME
D WELD/SEAM
Q HOSE/PIPING
D INNER LINER
G OTHER __ „ .

IX. OCSCniPTION OF EVENTS) Dojcriba tha ttquanea of «v»n« that lad to lncid«nt, action taken at t'm« di«cou»r*rl, anH ocrlnn taken to prevent futura
Incidents. Includa any racammandatloni to Improve packaging, handling, or iranjportation of hazardous material*. Photograph! and diarjrums f hould
b« tubminad when necemry for clarification. ATTACH A COPY OF THE HAZARDOUS WASTE MANIFEST FOR INCIDENTS INVOLVING
HAZARDOUS WASTE. Continue on additional theotc if necettary.
18  NAME OF PERSON RESPONSIBLE FOR PREPARING REPORT
47 SIGNATURE
48 TITLE OF PERSON RESPONSIBLE FOR PREPARING REPORT
49 TELEPHONE NUMBER (Af03 Coda)
                                                                                                      50. DATE REPORT

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                   oc 9"                 SECTION: APPENDIX S, ATTACHMENT 3
                   ""'                 VERSION: FINAL/MARCH 1997
                                       Attachment 3 - Cover
ATTACHMENT 3 - SAMPLE UNIFORM HAZARDOUS WASTE MANIFEST

-------
                       DNR&
            MICHIGAN DEPARTMENT
           OF NATURAL  RESOURCES
                                                       DO NOT WRITE IN THIS SPACE
                                                ATT. D      DIS.  D      REJ. D     PR.D
    Elease.pnm or type
            ^tt^ui'DJ _  ^dt aulno'lty 0' Acl b4
            '979 as amenoao ana Act 136  PA
            1969

            Failure to Me 13 punishaole unoer
            sechon 299 548 MCl O' Seclion 1C
            ACI 136 PA 1969
           UNIFORM HAZARDOUS
              WASTE MANIFEST
                                                                                                  Form Approved  OMB No 2050-0039 E«p»
                                        i Generator sUS EPA ID No
        3  Generator's  Name and  Mailing Address
        4  Generator's  Phone |
                                                                            Manifest
                                                                         (Document No
2 Page 1

  of
Information in the shaded ,
is  not required  by  FedeT
law
                                                                                       A. State Manifest Document Number
                                                                                                       2756776
                                                                                               B. State Generator's ID
           Transporter  1  Company Name
                                                                US EPA 10 Number
                                                                                               C. State Transporter's ID
           Transporter 2  Company Name
                                                                                              D. Transporter's Phone
                                                               US EPA 10 Number
                                                                                               E. State Transporter's ID
                                                            •tr
                                                                                              F. Transporter's Phone
IT  Designated  Facility Name and  Site Address
                                                                       US EPA 10 Number
                                                                                              G. State Facility's ID
                                                                                              H. Facility's Phone
        11. US DOT Description (including Proper Shipping Name, Hazard Class, and
           HM                       ID NUMBER).
12.Containers

  No    Type
                                                                                                 13
                                                                                                Total
                                                                                               Quantity
                   14.
                   Unit
               Waste
               No.
       J.   Additional Descriptions for Materials Listed Above
                                                                                      K. Handling Codes for Wastes
                                                                                        Listed Above
                            a/     /
                                                                                                                           b/    /
                                                                                                                          c/    /
                                                                                                                          til    I
       15. Special  Handling  Instructions and  Additional  Information
       16. GENERATOR'S CERTIFICATION: I hereby declare that the contents of this consignment are fully and accurately described above by
          proper shipping name and are classified, packed, marked, and labeled, and are in all respects in proper condition for transport by highway
          according to applicable international and national government regulations.

          If I am a large quantity generator. I certify that I have a program in place to reduce the volume and toxicity of waste generated to the degree I have determine*
          to be economically practicable and that I have selected the practicable method of treatment, storage, or disposal currently available to me which minimizes th
          present and future threat to human health and the environment; OR; if I.am a small quantity generator, 1 have made a good faith effort to minimize my wasli
          generation and select the best waste management method that is available  to me and that I can afford.                      	

                                                                                                                     I      Date
          Printed/Typed  Name
                                                          Signature
                       Month  Day  Ye<
       17. Transporter  1  Acknowledgement of Receipt  of  Materials
                                                                                                                     Oate
          Printed/Typed  Name
                                                          Signature
                       Month  Day  Yei
       18 Transporter  2  Acknowledgement or Receipt  of  Materials
                                                                                                                            Oate
"=2
          Printed/Typed Name
                                                          Signature
                       Month Day  Yet
       19. Discrepancy Indication Space
       20. Facility Owner or Operator Certification of receipt of hazardous materials covered by this manifest except as noted in
          Ham  19
                                                                                                                            Dal-
          Printed/Typed  Name
                                                          Signature
                                                                                                                      Month Day  Yea
    PPA Pnrm «7On.99 I Oou Q/flBt

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                                                      SECTION: APPENDIX U
                                                      VERSION: FINAL/MAY 1997
                                                      PAGE: COVER
APPENDIX U: SURVEY FOR NON-FRIABLE ASBESTOS CONTAINING MATERIALS

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              DRAFT
    NON-FRIABLE ASBESTOS
OPERATION AND MAINTENANCE
              PLAN

             Prepared for
            USEPA GLNPO
          R/V LAKE GUARDIAN
                By

         Melody A. Pieper, CHMM
             March 1995

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INTRODUCTION AND BACKGROUND

Asbestos-Containing Material (ACM)  was  identified  on board the R/V
Lake Guardian  in  1994.   This  Asbestos  Operations  and Maintenance
Plan is a direct result of the identification of this material, and
represents  a  sound,  safe  approach  to managing the  potential
exposures associated with non-friable asbestos.

Friable Asbestos-containing materials may present a health hazard
to occupants if airborne asbestos fibers are inhaled. Non-friable
materials pose no health hazards, provided they are maintained in
an intact and un-damaged condition.  The objective of the plan is
to identify the location of the ACM and to notify  occupants of the
presence  of  the material,  and to ensure that the  material is not
disturbed or damaged.

An Asbestos Operation and Maintenance Plan is an  active document,
and may be revised as necessary to accommodate changing conditions
and regulatory requirements.

Asbestos  Health Hazards

Asbestos  is the name of a class of magnesium-silicate minerals that
occur in fibrous form, including chrysotile,  amosite, anthophyllite
asbestos, tremolite asbestos,  and actinolite asbestos.   Asbestos
fibers   are   fire-resistant,   chemical-resistant   and  abrasion-
resistant and  have  been  extensively used in the manufacture of a
wide variety of products.

Microscopic  fibers  can  be released to the air  from  asbestos-
containing materials  (ACM) when  they are damaged  or deteriorated.
Friable  materials  can  be crumbled  with hand pressure,  and are
likely  to  release  fibers into the  air.    Fibrous or  fluffy
materials, such as  sprayed-on fireproofing or pipe insulation, are
considered  friable, and  can readily release fibers if disturbed.
Materials such as vinyl-asbestos floor tile,  eisphalt roofing felts,
and transite are considered to be non-friable and  generally do not
release  airborne fibers unless they  are  subjected  to sanding,
grinding or  sawing  operations.

Asbestos fibers  can cause a disabling respiratory  disease and
various  types  of cancers if the fibers are inhaled while airborne.
Breathing or ingesting fibers from contaminated  clothing or skin
can  also result in these diseases.  Many of the  asbestos related
diseases have  a  latency period  of  20-30  years  after initial
exposure. The most common lung disease is asbestosis, or scarring
of the lining of the  lung, which can cause shortness of breath,
coughing,  fatigue,  and  other  symptoms.     Lung  cancer  and
mesothelioma  are   also,  more  rarely,  associated  with asbestos
 exposure, and can cause  shortness  of breath  and pain  in the walls
 of the  chest  or  abdomen.    Lung  cancer  is  usually  fatal and
mesothelioma is always fatal.

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Asbestos on the Lake Guardian

In  1994,  it was  assumed that  the  lining of  one of  the ship's
chemical  storage  cabinets  contained  a  non-friable  asbestos-
containing material, commonly referred to as transite.  In a non-
friable state this material poses no health risks.  However,  it  is
important that  the material remain in an intact  and un-damaged
condition.  Additionally, no persons shall be permitted to grind,
sand, or  saw into  this material,  or in any  other way damage this
material thus rendering the material friable.

Options For Control Of Asbestos

Encapsulation

Since the ACM  is classified as  non-friable,  and is in excellent
condition,  the  current condition of the  cabinet lining poses  no
health  hazard.   Additionally,  for extra protective measures, the
cabinet lining has been encapsulated with an epoxy material.

Inspections

An  on-going inspection program  of  the encapsulated material has
been  established  on  a  semi-annual basis,  to  ensure  that the
encapsulated material  does not become  damaged.

Statement of Objectives

The objectives of  USEPA GLNPO are:

     To prevent exposures  of occupants  to  unhealthy  levels  of
     airborne fibers,

     To remove or  repair  all deteriorated ACM as soon as  possible
     using  control methods necessary  to protect human health and
     the  environment,

     And  to minimize damage to existing ACM.

Overall Strategy

Vessel  occupants will be notified  of  the Asbestos Operation and
Maintenance Program.   No  one will be permitted to grind,  sand,  or
drill into  the lining  of the cabinet,  or in any way  damage the ACM
without specific approval from the Asbestos Manager.   Should  it  be
necessary to perform work activities which may in some way damage
the material,  thus creating  the  potential  for the  release  of
fibers,   appropriate preventative measures  will  be  implemented.
Such measures  would  include:  ensuring persons  performing work
activities  are  properly  trained/certified  to  work with  ACM,
issuance  of appropriate PPE, use of wetting methods, and use  of
negative  pressure enclosure when applicable.

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Personnel Involvement

Asbestos Manager/Inspector

The Asbestos Manager/Inspector duties consist of ensuring that all
portions of this program are  implemented.  Additionally, it is the
Asbestos  Manager/Inspectors  responsibility  to perform  required
semi-annual  inspections in  accordance with  the  program.    The
Inspector  must be trained/certified in the inspection of ACM.

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                                                        SECTION: APPENDIX V
                                                        VERSION:  FINAL/MAY 1997
                                                        PAGE: 1 of 8
Appendix V - ANNUAL AND PERIODIC INSPECTIONS AND SURVEYS
1.      STANDARDS/COMPREHENSIVE PROGRAM

For all inspections or surveys conducted in Region  5, standards and regulations will  apply  in the
following order of importance:

       1)     EPA regulations in CFR form or as EPA Orders or regulations
       2)     OSHA regulations contained in various CFRs (e.g. 29 CFR 1910, 29 CFR 1926)
       3)     All  applicable  laws  and  regulations  including  those  from   Department  of
              Transportation, the Coast Guard, state and local environmental laws and regulations, in
              addition to consensus programs  such as the National Fire Protection Association and
              the American National Standards Institute.

All appropriate standards and regulations will  be used to assess and evaluate hazardous conditions
during inspections.  If a conflict exists in  the  application of various standards and  regulations, the
Regional Health  and  Safety  Manager will determine  which standard or regulation  applies based on
professional judgment and risk assessment techniques.

A  summary of required  inspections, frequency of inspections, and personnel responsible for specific
inspections can be found in the table below.
       SAFETY INSPECTIONS AND SURVEYS REQUIRED OF GLNPO

PERIOD          INSPECTOR                           TITLE

Weekly           Facility Safety Officer            General Safety Inspection

Weekly           Ship Operations Contractor        Drinking Water Inspections

Weekly           Facility CHO/Safety Officer       Hazardous Waste Storage Area Inspections

Weekly           Lab Safety Officer               Laboratory Eyewash and Safety
                                                 Shower Inspection

Monthly          Ship Operations Contractor        Ship Fire Alarms

Wkly/Monthly     Facility Safety Officer and        Fire Extinguisher & Fire Hydrant
                  Ship Operations Contractor        Inspections

Monthly          Safety Officer and               SCBA Inspections
                  Ship Operations Contractor

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                                                         SECTION: APPENDIX V
                                                         VERSION: FINAL/MAY 1997
                                                         PAGE: 2 of 8
PERIOD          INSPECTOR

Monthly          Ship Operations Contractor

Semi-annual       EPA Staff


Semi-annual       Outside Contractor

Semi-annual       Ship Chemical Hygiene
                  Officer

Annual            EPA Industrial Hygiene
                  Consultant

Annual            EPA Staff/Consultant

Annual            Ship Operations Contractor
                  and Outside Consultant

Annual            Naval Hospital - Great Lakes, IL

Annual            Naval Hospital - Great Lakes, IL

Annual            Naval Hospital - Great Lakes, IL

Periodic          CHOL Safety Officer

5 Year            Outside Contractor

Prior to each tour  Ship Operations Contractor
       TITLE

Life Safety Equipment Inspections

Safety and Health and
Environmental Compliance Surveys

Ship Fire Alarms

Laboratory  Hood Inspections


Industrial Hygiene Surveys


Environmental Compliance Audit


Crane and Rigging Inspections

Occupational Health Inspection

Preventive Medicine Inspection

Sanitation (and refrigeration) Inspection

Sanitation Card Refrigeration Inspection

Hull Inspection

Open House Public Safety Checklist
2.     SEMI-ANNUAL   SAFETY.    HEALTH.   AND  ENVIRONMENTAL    COMPLIANCE
       SURVEYS

As required  by EPA  Order  1440,  all Region  5 GLNPO  facilities,  spaces, vehicles, vessels, and
equipment will be inspected or evaluated by the Regional Health and Safety Manager.  The purpose of
these inspections is to assess, evaluate and reduce hazards, as well  as to ensure the effectiveness of
Safety Programs throughout GLNPO.

All inspections will be followed by a written Safety Inspection Deficiency Notice  Report, stating the
location of the inspection (division, branch, type of occupancy, and  so on), the hazards observed, the
applicable regulations or  standards, the risk assessment of the hazard (serious, non-serious, etc.), and
the necessary corrective measure(s).

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                                                          SECTION: APPENDIX V
                                                          VERSION:  FINAL/MAY 1997
                                                          PAGE: 3 of 8
A  cover  letter to the Division or Office Director will accompany the report and will require a first
abatement report to be submitted within 45  days  with  subsequent  reports every 45 days until all
deficiencies are corrected or abated.  All abatement  or hazard correction  reports should be sent to the
Regional Health & Safety Manager.
3.     ANNUAL INDUSTRIAL HYGIENE SURVEYS

Yearly Industrial  Hygiene Surveys are required for all EPA laboratory facilities.  The purpose of these
surveys is to evaluate and reduce occupational health hazards in Regional laboratories and to evaluate
the overall Occupational  Health Program in these laboratories.  These surveys will be conducted by a
qualified Industrial Hygienist under the  overall direction of the Regional Health and Safety Manager.
Survey reports will be sent to the  Division or Office  Director. A cover letter  containing comments
relative to the overall Occupational  Health Program of the facility will  be  attached to the survey.  In
addition, the cover letter will provide recommended abatement procedures.
4.     ANNUAL ENVIRONMENTAL COMPLIANCE AUDITS

Regional Environmental Compliance Audits will be conducted as part of the Annual Regional Safety
Inspection.   Environmental compliance  findings,  such as the use,  transportation, and  disposal of
hazardous waste,  will be included in the  Safety Inspection Deficiency Notice Report.  Environmental
compliance findings will be labeled to distinguish them from safety findings.
5.     SPECIAL STUDIES

Special  studies  relating to  safety,  occupational  health  or environmental  compliance  issues are
conducted yearly at the discretion  of the Regional Health  & Safety Manager.  Most of these studies
involve occupational health issues  such as indoor air quality or  use of rare  or unusual chemicals and
are issues cited  by  management or employees  in a specific work  area.  Under  the  direction of the
Regional Health & Safety Manager, all special studies are  conducted by qualified professionals in the
subject being studied (e.g. Industrial  Hygienist,  Environmental Protection  Specialist, etc.).  Results of
the studies will be shared with all concerned employees and management.  If abatement procedures are
required,  the  Regional  Safety  Manager  will coordinate  such  activities with the  division or office
concerned.
6.     DEFICIENCY ABATEMENT PROCEDURES AND TRACKING

All deficiencies or hazards noted in a Semi-annual Inspection  Report must be followed by subsequent
reports every 45 days from receipt of the initial report until  all  deficiencies are corrected  or abated.
Each  abatement  report from the Division or Office Director  need only refer to those deficiencies or
hazards which were detailed in the  previous abatement report.

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                                                         SECTION: APPENDIX V
                                                         VERSION: FINAL/MAY 1997
                                                         PAGE: 4 of 8
Once deficiencies are reported to the Regional Health and Safety Manager in an abatement report from
a division or office,  each  individual deficiency or hazard,  its  cost,  expected time of abatement and
other related  factors  are tracked by an automated  Deficiency or Hazard Abatement System.  This
tracking system will generate a letter to the appropriate division or office every 45 days if Abatement
Reports are not received.  This system will also enable the Regional Health and Safety  Manager to
inform  senior management of abatement  status and percentage of hazards abated and other related
factors  relative to a given division, office or the Region overall.
7.      FIRE PROTECTION SYSTEMS STANDARD OPERATING PROCEDURES

To ensure that GLNPO research vessel  fire protection systems are ready for use at all times and that
all equipment provided is properly maintained, fire protection systems standard operating procedures
must be developed and implemented.  A  standard checklist must be completed for each inspection and
the checklist must be signed by the inspector.

The  following areas are  included in Attachment 1 and must  be inspected in accordance with all
regulatory and Standard requirements, including NFPA Standards and 46 CFR Part 189:

       •Fire Detection And Alarm System
       •Fire Alarm Check List
       •Monthly Fire Extinguisher Inspection Check List
       •Weekly Fire Extinguisher Inspection Check List
       •Container Lab Extinguishing Systems
       •Galley Extinguishing Systems
       •Emergency Lighting Systems
       •Fire Pumps, Main Hose, Hose Stations
       •Engine Room Carbon Dioxide System
8.     SHIP FIRE ALARMS/SEMI-ANNUAL INSPECTIONS BY OUTSIDE CONTRACTOR AND
       MONTHLY INSPECTIONS BY SHIP OPERATIONS CONTRACTOR

Comprehensive inspections of GLNPO research vessel fire alarm systems will  be performed semi-
annually  by  qualified  contractor(s).   The contractor(s) must be approved by  the Regional  Safety
Manager  prior to  inspection activities.   In  addition  to semi-annual  inspections,  comprehensive
inspections will be performed on a monthly basis by the ship operations contractor.

Semi-annual  and  monthly fire  alarm  inspection  procedures  are  located in Attachment 1.1 of this
chapter.   A  comprehensive  fire alarm checklist  for the R/V Lake  Guardian, to be  used by  the
inspector, is located in Attachment 1.10 of this chapter.

All records of inspection must be maintained on  board each vessel inspected.   Copies of the records
must also be  maintained at GLNPO located in Chicago, Illinois..

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                                                          SECTION: APPENDIX V
                                                          VERSION: FINAL/MAY 1997
                                                          PAGE: 5 of 8
9.     MONTHLY FIRE EXTINGUISHER AND FIRE HYDRANT INSPECTIONS
       9.1  Inspection:  Extinguishers will be inspected when first placed into service and  inspected
       at 30-day intervals thereafter.  Sometimes, extinguishers will be inspected more  frequently as
       situations and circumstances warrant.  The inspection procedure for monthly fire extinguisher
       inspections is found in  Attachment 1.3 of this chapter.  NOTE:  In addition to the required
       monthly fire extinguisher inspections, weekly fire extinguisher inspections will be performed
       by the ship  operating contractor.  A weekly inspection checklist is also found in Attachment
       1.4 of this chapter.
       9.2  Corrective Action:  When inspection of an extinguisher indicates a deficiency  listed on
       the inspection checklist, immediate corrective action will be taken.
       9.3  Rechargeable Extinguishers:  When  inspection of a rechargeable extinguisher indicates a
       deficiency  listed  on the checklist, appropriate maintenance  procedures will be  immediately
       taken.
       9.4  Non-rechargeable Dry Chemical Extinguisher:   When inspection of a non-rechargeable
       dry  chemical extinguisher indicates  a deficiency  among the checklist  items,  it will be
       discharged and removed from service.
       9.5  Recordkeeping:  Records will include the following: the date of inspection, the inspectors
       name,  and any deficiencies  noted.  Inspectors will remove deficient fire extinguishers from
       service until repairs are made.  Temporary replacement extinguishers must be used to provide
       continuous protection.
10.    ANNUAL AND PERIODIC CRANE AND RIGGING INSPECTIONS

Annual  inspections are required  for  all derricks,  cranes,  hoists or rigging.   Inspections will  be
performed in accordance with OSHA 29 CFR 1910.179-181  by an outside certified contractor.
11.    WEEKLY INSPECTIONS OF EYEWASH AND SAFETY SHOWERS

Weekly inspections will be performed to ensure the proper working condition of eyewash stations and
safety showers.  Eyewash stations and showers must be maintained in accordance with ANSI Z 358.1.
A  weekly inspection checklist appears as Attachment 2 of this chapter.

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                                                         SECTION: APPENDIX V
                                                         VERSION: FINAL/MAY 1997
                                                         PAGE: 6 of 8
12.     ANNUAL   AND   PERIODIC   OCCUPATIONAL   HEALTH/PREVENTIVE
       MEDICINE/SANITATION INSPECTIONS

Annual  and periodic occupational  health, preventive  medicine, and  sanitation  inspections  will be
coordinated by designated personnel. This inspection will be performed by the U.S. Navy or qualified
equivalent.  In addition, on vessels providing food service,  periodic food service inspections must be
performed in accordance with the standard operating procedures specified in the Standard Operating
Procedures  for Vessel   Sanitation (Attachment  3 of this  chapter).  The Food Service Inspection
Checklist, Attachment  4 of this chapter, should also be completed.  Periodic in-house inspections will
also be conducted by the ship's Safety Officer.  Certificates of Acceptance for the annual and periodic
inspections should be  kept on  board  GLNPO research  vessels and  copies should be  provided to
GLNPO in Chicago, Illinois.

13.     LIFE SAFETY  EQUIPMENT INSPECTIONS

Inspections of all life safety equipment,  such as  life rafts and  escape equipment, on board GLNPO
research  vessels will be inspected monthly by the ship  operations contractor.   The inspections are
designed to ensure  safe operation of all  life safety equipment in the event of an emergency.   A
drawing, showing the locations  of escape equipment, has been posted in the galley, and within the
passageways to the labs; see Attachment  1.11 of this chapter.

Records  of inspection  must be  maintained  on  board  each  research  vessel  and  at  GLNPO  in
Chicago, Illinois.

14.     SEMI-ANNUAL LABORATORY HOOD  INSPECTIONS AND REPORTS

The  EPA policy  for laboratory hood face velocities is 100 cubic feet per minute  (cfm).  GLNPO
research vessels' fume  hoods must be tested for adequate face velocity on a semi-annual  basis by the
ship's  Chemical Hygiene  Officer.   An evaluation form  appears  as Attachment 5 of this  chapter.
Copies of the semi-annual  laboratory hood inspection and report must be sent to EPA headquarters in
Washington, D.C.

15.     DRINKING WATER INSPECTIONS

Inspections of potable drinking water systems on  board GLNPO research vessels will be performed in
accordance with all  regulatory and  policy requirements.   The  inspections will  be performed by the
ship's operations  contractor or Health and Safety  personnel.

EPA policy  requires that  EPA facilities  monitor drinking water for  lead  and copper  and take
appropriate  action if  levels  are at or above the action  level.  GLNPO  complies  with this policy
through  monitoring  of shipboard drinking fountains and  sink faucets  that are used as sources of
drinking water.

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                                                        SECTION: APPENDIX V
                                                        VERSION: FINAL/MAY 1997
                                                        PAGE: 7 of 8
If a research vessel  contains a potable water supply,  monitoring  for coliform and Free Available
Chlorine (FAC) will  be conducted by the ship operations contractor or Health and Safety  Personnel.
Frequency of testing will be determined based the vessels usage and the frequency  of taking  on
potable water.  However,  the period of time between testing should  not exceed one month.  Sampling
data sheets can be found as Attachment 10 of this chapter.

Copies of all inspection records will be maintained on board each vessel inspected and at GLNPO in
Chicago, Illinois.

16.    MONTHLY SCBA INSPECTIONS

In case of emergency, a self-contained breathing apparatus (SCBA) is used as respiratory protection in
hazardous  or oxygen-deficient  atmospheres.  Monthly  inspections must be conducted to ensure the
readiness of the SCBA.  A generic inspection checklist appears as Attachment  6  of this  chapter.
Additionally, ten minute escape devices must be inspected monthly.
17.    WEEKLY HAZARDOUS WASTE STORAGE AREA INSPECTIONS

Inspections of hazardous waste storage areas will be conducted on a weekly basis. This inspection will
confirm that hazardous wastes are stored  in  segregated areas and in leak-free,  properly-labeled
containers with no evidence of spillage.  Improper storage or extended accumulation dates will be
immediately noted and corrected.  The Weekly Hazardous Waste Storage Area Inspection Sheet and
Spill Cart Inspection Form appear as Attachments 7 & 8 of this chapter.

18.    EMERGENCY SUPPLIES AND EQUIPMENT INSPECTIONS/RCRA REQUIREMENTS

The Chemical Hygiene Officer is responsible for ensuring that  inspections of all emergency supplies
and equipment are performed in accordance with applicable RCRA generator requirements.  Also, as
required  by  RCRA,  locations  of  shipboard  emergency  supplies and  equipment  will be  posted
throughout all operational areas where hazardous wastes are generated and stored.

19.    HULL INSPECTIONS (5-YEAR DRY DOCK REQUIRED BY U.S. COAST GUARD)

All US EPA research vessels must comply with the US Coast Guard and the American Bureau of
Shipping five-year  dry-docking  and overhaul inspection  requirements.   Inspection Specifications are
included in Attachment 9 of this Chapter; "Specifications for Dry-Docking and Overhaul Inspection".
20.     SAFETY CHECKLIST FOR IN-PORT OPEN HOUSES

Prior to in-port open house activities, the ship operations contractor must ensure that the ship is safe
and secure  for visitors by completing the "Pre-Open House Checklist"  The  checklist is explained in
more detail in Chapter 8 of this manual.

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                                                        SECTION: APPENDIX V
                                                        VERSION: FINAL/MAY 1997
                                                        PAGE: 8 of 8
21.     SANITATION AND FOOD HANDLING INSPECTIONS

Sanitation and refrigeration inspections will be conducted annually by an outside contractor (eg. Naval
Hospital).   Sanitation standard operating procedures appear as Attachment 3 of this chapter and  a
food handling inspection form appears as Attachment 4.  Additionally, periodic inspection will be
conducted by the ship's health and safety personnel.
22.    DOCUMENTATION

All inspection records that are completed for GLNPO research vessels must be filed on board each
vessel inspected.  Copies of all inspection records  must also be provided to the GLNPO  in Chicago,
Illinois.

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                                           SECTION: APPENDIX V, ATTACHMENT 1
                                           VERSION:  FINAL/MAY 1997
                                           Att.  1 - Page 1

Attachment 1 FIRE PROTECTION SYSTEMS - STANDARD OPERATING PROCEDURES

1.0 SCOPE

These Standard Operating Procedures contain the necessary information for the testing and inspection
of all vessel fire protection systems. It is intended to ensure  that the systems will be ready for use at
all times and that all equipment provided is maintained and replaced after each use or needed repairs.
The procedure requires that  a  checklist be completed for each inspection.  The checklists detail  the
extent of the inspection made  and must be signed,  upon completion, by the person  performing  the
inspection.   These checklists will serve as a record of the testing performed on the fire protection
systems.

2.0 REFERENCES

The recommended procedures  for the testing of the various  fire  protection  systems have been taken
from the USCG regulations  and NFPA Standards.   The criteria applicable to fire protection  systems
has been developed from the  following:

       46 CFR Part  189 -      Oceanographic and Research Vessels

       NFPA Standards -      NFPA 10 - Fire extinguishers, Portable

       NFPA 20 -            Fire Pumps, Centrifugal

       NFPA 14A -           Standpipe and Hose Systems, Inspection, Testing and Maintenance

       NFPA 12 -            Carbon Dioxide Extinguishing Systems

       NFPA 17A -           Wet Chemical Extinguishing Systems

       NFPA 17 -            Dry Chemical Extinguishing Systems

       NFPA 72H -           Testing  Procedures   for  Local,  Auxiliary,   Remote  Station,  and
                             Proprietary Protective Signaling Systems

       NFPA 101 -           Life Safety Code (Emergency  Lighting)

3.0 RESPONSIBILITIES AND AUTHORITY

It  is the duty of the Captain, or  person  designated by  the Captain, to ensure that   fire  fighting
equipment aboard an EPA research vessel is ready for use  at all times and that such equipment  is
provided, maintained, and  replaced after use in  accordance  with proper procedure.  No changes  or
repairs can  be made to any fire protection system without the Captain's knowledge and consent.  All
fire protection equipment must be  100% operational before the vessel will be permitted to leave the
dock.  When repairs or modifications are made to any fire protection system, and the system cannot be
returned  to full operational  status,  the condition shall be  so  noted  and  explained in the vessel's log
book.    Interim  compensatory measures, such  as  a  roving  fire  watch  or additional   portable
extinguishers, shall be provided, at the Captain's discretion, until the  system can be fully repaired.

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                                           SECTION: APPENDIX V, ATTACHMENT 1
                                           VERSION:  FINAL/MAY 1997
                                           Alt. 1 - Page 2
4.0 EQUIPMENT REQUIRED

Most  of the  required  tests  and inspections can be  performed with ordinary tools and equipment
available on board the vessel.  Where some pieces of specialized hardware is required, it is listed in
the specific procedure for the system being inspected.
5.0 PROCEDURE

This procedure contains the written instructions for the testing  and maintenance of the  vessel's fire
protection systems.  Each system is provided with  separate instructions and checklists.  The frequency
of the  inspections is noted within each procedure.   Typically, a monthly and annual or semi-annual
inspection is required.   The attached  comprehensive Fire Inspection Standard Operating Procedures
have been developed and should be completed as indicated by qualified fire inspectors.

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                                        SECTION: APPENDIX V, ATTACHMENT 1.1
                                        VERSION: FINAL/MAY 1997
                                        Act. 1.1 - Page 1
ATTACHMENT 1.1 FIRE DETECTION AND ALARM SYSTEM
Procedure - Monthly

The fire detection systems shall be inspected monthly, or at approximately 30-day intervals.
Extension of the schedule up to one week  is permitted  in  unusual  circumstances.   The
inspection should include the following:

       1)    The green "Power  "On" indicator lamp should be illuminated.

       2)    The control panel lamp test switch should be depressed to verify that all
             lamps are functional.

       3)    No yellow trouble  lamps or red alarm lamps should be illuminated.

       4)    The battery trouble lamp should not be illuminated.

       5)    The voltage meter  should be above 24 volts.

       6)    No obvious damage, disconnected wires, or altered circuits should be
             evident.

       7)    One device  should be  randomly activated to ensure that the system is
             functional and the  alarms are audible throughout the vessel.

When any deficient condition  is found, it shall be listed on the checklist, and steps taken to
correct the deficiency.  The person performing the inspection should then complete, sign, and
date the attached checklist.

Procedure - Semi-annuallv

The Fire Detection and Alarm system must be inspected and serviced semi-annually by a
qualified service technician.  The inspection should include those items specified by NFPA
72H.

-------
                                       SECTION: APPENDIX V, ATTACHMENT 1.1
                                       VERSION: FINAL/MAY 1997
                                       Att. 1.1 - Page 2
CHECKLIST - FIRE DETECTION AND ALARM SYSTEMS
Location            Bridge_
                    Lab 1A_
                    Lab 2A_
                    Lab 3A_
                    Lab 4A
The green "Power On" indicator lamp is illuminated

Lamp test shows that all lamps are functional

No yellow trouble or red alarm lamps are illuminated

The battery trouble lamp is not illuminated

The voltage meter reads above 24 volts

No obvious damage, disconnected wires, or altered circuits

Location and type of device tested

All alarms audible

Comments:	
Inspected By                                   Date

-------
                                           SECTION: APPENDIX V, ATTACHMENT 1.2
                                           VERSION:  FINAL/MAY 1997
                                           Att. 1.2 - Page 1
Attachment 1.2 FIRE ALARM CHECK LIST

A semi-annual test of the fire alarm system aboard all GLNPO vessels is required.

One sixth of all smoke detectors must be inspected monthly.  Each monthly inspection will comprise a
different group of detectors ensuring that each detector is inspected semi-annually.

The fire alarm checklist must include the following items:

       •      All smoke detectors must be identified by location.

       •      All pull stations must be identified by location.

       •      All heat detectors must be identified by location.

       •      Carbon dioxide pressure switches (usually found in engine rooms) be  identified by
              location.

Comments and discrepancies  must be addressed at the end  of the checklist and the  inspector's
signature and date of inspection must appear on the form.

-------
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-------
                                        SECTION: APPENDIX V, ATTACHMENT 1.3
                                        VERSION:  FINAL/MAY 1997
                                        Att.  1.3 -Page 1
ATTACHMENT 1.3
Inspection will be performed in accordance with NFPA Standard No. 10.
                         MONTHLY  INSPECTION CHECKLIST
                                         FOR
                                FIRE EXTINGUISHERS
Date:
Fire Extingui
Y N
n a
a
a
a
a
a
n
a
a
a
a
isher Number (#):
Located in designated place.
No obstruction to access or visibility.
Operating instructions on nameplate legible and facing
Seals and tamper indicators not broken or missing.
Determine fullness by weighing or "hefting".
Examine for obvious physical damage, corrosion, leake


outward.


ige, or ck
             nozzle.

n     D     Pressure gauge reading or indicator in the operable range or position.

Comments/Discrepancies:	
Signature:

Date:

-------
Attachment 4.1.4                                                 Final May 1997




          WEEKLY FIRE EXTINGUISHER INSPECTION CHECKLIST
Station #
1
2
3
4
5
6
7
8
'
j—
10
11
12
.3
ft
r i5
16
17
18
19
20
21
22
23
24
25
26
/If OK


























Comments


























Action Taken If Needed


























Location
Bridge AFT
Bridge FWD
Outside - AFT Doghouse
0-1 Level - FWD Passageway
0-1 Level - AFT Passageway
0-1 Level Outside AFT Bulkhead
Mess Deck FWD
Galley
Main Deck Outside Crew Quarters
Main Passageway Outside Chemlab
Inside Chemlab - Stbd Wall FWD
Inside Chemlab- Stbd Wall AFT
Biology Lab - Port wall FWD
Biology Lab - Port wall AFT
Wet Lab - Starboard Side
Wet Lab - Port Side
Rear of Bowthruster
Outside MSD Room Portside -
Lower Passageway
Outside Aux. Machine Room -
Portside Passageway
Machine Shop - FWD of Lathe
Machine Shop - AFT Bulkhead
Engine Room FWD at Entrance
Engine Room Starboard Side
0-1 Level Outside AFT
Engine Room Port Side
Exercise Room Starboard Side
Initial


























Week Ending.
Signature.

-------
Attachment 4.1.4
WEEKLY FIRE EXTINGUISHER INSPECTION CHECKLIST
»-
2
3
4
5
6
7
8
9
10
11
12
13
U4
F~
17
18
19
20
21
22
23
24
25
26
27
28
29
•J
Location
Bridge Deck AFT (outside)
Bridge Deck FWD (outside)
Emerg. gear locker PORT
(Spare extinguishers)
Bridge AFT (inside)
Bridge FWD (inside)
Focsle Deck Passageway FWD
Captain's Cabin
Focsle Deck Passageway AFT
Focsle Deck STBD (Rosette)
Focsle Deck HVAC
Mess Deck FWD
Galley Sink
Galley AFT
Main Deck Passageway FWD
Main Deck Passageway AFT
Chem lab FWD
Chem lab AFT
Bio lab FWD
Bio lab AFT
Wet lab FWD
Wet lab AFT
Exercise Room
Bow Thruster Room
MSD Room (Passageway)
Mach. Shop FWD (Lathe)
Mach. Shop AFT
Aux.Mach.Rm. (Passageway)
Engine Room FWD
Engine Room STBD
Engine Room PORT
Type
CO2
CO2
1-CO2
2-ABC
CO2
ABC
C02
ABC
CO2
CO2
CO2
CO2
ABC
CO2
CO2
CO2
ABC
ABC
ABC
ABC
ABC
ABC
C02
CO2
C02
ABC
CO2
CO2
CO2
CO2
CO2
Date






























/if OK


















"











Comments or Action Taken






























Initial






























Week Ending_
                   Signature_

-------

-------
                                           SECTION: APPENDIX V, ATTACHMENT 1.5
                                           VERSION:  FINAL/APRIL 1997
                                           Att. 1.5 - Page 1
Attachment 1.5 - CONTAINER LAB EXTINGUISHING SYSTEMS


Procedure - Monthly

The Container lab fire extinguishing systems shall be inspected monthly, or at approximately 30-day
intervals.  Extension of the schedule up to one week is permitted in unusual circumstances.  The
inspection should include the following:

       1) Cylinder should be securely fastened to the bulkhead.

       2) Manual release and nozzles should be unobstructed.

       3) Tamper indicators and seals should be intact.

       4) No obvious physical damage should be present.

       5) Pressure gauge should  be  in the operable range.

When any deficiency is found,  it  shall be noted on the  checklist, and steps shall be taken to correct the
condition. The person performing the inspection should complete, sign, and date the attached
checklist.


Procedure - Annually

The lab extinguishing system must be inspected and serviced by a qualified technician. The inspection
should include those items  specified by NFPA 17.

-------
                                        SECTION: APPENDIX V, ATTACHMENT 1.5
                                        VERSION:  FINAL/APRIL 1997
                                        Alt. 1.5 -Page 2
CHECKLIST - CONTAINER LAB EXTINGUISHING SYSTEMS


Location             Lab 1A	

                    Lab 2A	

                    Lab 3A	

                    Lab4A
The cylinder is in place and securely fastened to the bulkhead:

The manual release station and the nozzles are unobstructed:

The tamper indicators and seals are intact:

There is no obvious physical damage or corrosion:

The pressure gauge is in the operable range:

Comments
Inspected By	  Date_

-------
                                            SECTION: APPENDIX V, ATTACHMENT 1.6
                                            VERSION: FINAL/MAY  1997
                                            Att. 1.6 - Page 1
Attachment  1.6 - GALLEY EXTINGUISHING SYSTEM
Procedure - Monthly

The galley fire extinguishing system shall be inspected monthly, or at approximately 30-day  intervals.
Extension of the schedule up to one week  is permitted  in unusual  circumstances.   The inspection
should include the following:

        1)      Red lever in the control head  should  be  horizontal with the pin in place,  and the
               system indicator positioned in the "set", not the "released", position.

        2)      Manual release should be unobstructed with the pin in place.

        3)      Cylinder pressure gauge should be in the green operable range.

        4)      There  should be two nozzles  on the piping above the range with blowoff caps  in place.

        5)      There  should be no visible signs of damage to the system, and the cylinder and piping
               should be securely fastened to the bulkhead.

        6)      The fusible link should be in  place in  the hood above the range.

When any  deficient condition is found,  it shall be noted on the checklist,  and steps shall be taken to
correct  the deficiency.  The person performing the  inspection should complete, sign, and  date the
attached checklist.

Procedure - Annually

The galley extinguishing system must be  inspected and serviced by a qualified service technician.  The
inspection should include those items specified by NFPA 17A.

-------
                                          SECTION: APPENDIX V, ATTACHMENT 1.6
                                          VERSION: FINAL/MAY 1997
                                          Alt. 1.6 - Page 2
CHECKLIST  - GALLEY EXTINGUISHING SYSTEM


The red lever on the control head is horizontal with the pin in place:

The system indicator is in the "set" position, not the "released " position:

The manual release is unobstructed with the pin in place:

The cylinder pressure gauge is in the green operable zone:

There are two nozzles on the piping above the range:

Blowoff caps are in place;

There are no visible signs of damage to the cylinder and piping
and they are securely fastened to the bulkhead:

The fusible link is in place in the hood above the range:

Comments
Inspected By	   Date

-------
                                            SECTION: APPENDIX V, ATTACHMENT 1.7
                                            VERSION:  FINAL/MAY 1997
                                            PAGE: 1  of 2
Attachment  1.7 - EMERGENCY LIGHTING
Procedure - Monthly

The  emergency lighting units shall  be inspected  monthly,  or at approximately 30-day  intervals.
Extension of the schedule  up to one week is  permitted in unusual circumstances.  The inspection
should include the following:

        1)      The test switch should be  depressed  for  at  least 30  seconds.   The lamps  shall
               illuminate, and the charge indicator shall momentarily indicate rapid charge.

        2)      The lamps shall be aimed to illuminate the exit path.

When any deficient condition  is found,  it  shall  be listed on the checklist, and steps shall be taken to
correct  the deficiency.  The  person performing the inspection should complete,  sign, and  date the
attached checklist.
Procedure - Semi-Annually

On a semi-annual basis, each lighting unit should be tested by performing a deep cycle discharge test
by  unplugging  the unit.   If the unit  is deemed functional, it will be re-energized.  The lamps shall
remain illuminated for a period of 1-1/2 hours.  If the lamps do not remain illuminated for the full 90
minutes, the batteries shall be replaced.

This annual testing procedure will also serve as the monthly inspection for that particular month.

-------
                                    SECTION: APPENDIX V, ATTACHMENT 1.7
                                    VERSION: FINAL/MAY 1997
                                    PAGE: 2 of 2
CHECKLIST - EMERGENCY LIGHTING
Test Period
Monthly
Semi-Annual

Location














Test Switch














Charge Indicator














Aim Correct














Deep Cycle














Inspected By: Date:

-------
                                            SECTION: APPENDIX V, ATTACHMENT 1.
                                            VERSION:  FINAL/MAY 1997
                                            PAGE: 1 of 4
Attachment 1.8 - FIRE PUMPS. MAIN HOSE. HOSE STATIONS
Procedure - Weekly

The fire pumps, fire main, and hose stations shall be inspected weekly.  Extension of the schedule up
to one week is permitted in unusual circumstances.  The following should be checked:

       1)      Test each fire pump on a staggered basis.  Start the pump and operate on recirculation
               or allow to flow  through  a convenient  outlet.   Operate the pump  for at  least 10
               minutes and check  for abnormal conditions such as noise, vibration or leakage.

       2)      Visually  observe each valve on the fire main and verify that the valves are sealed in
               the open  position.

       3)      Inspect each fire hose and verify that:

                      • The gate  valve is closed
                      • The valve handle is present
                      • The hose and nozzle each have a gasket in place
                      • The hose is present and not damaged or mildewed
                      • A spanner wrench is present
                      • The nozzle  is present and the open/shut handle operates freely.
                      • There are no visible signs of leakage.

When  any  deficient condition is found,  it shall be listed  on the checklist,  and steps shall be taken to
correct the  deficiency.   The person performing the inspection should complete, sign,  and date the
attached checklist.
Procedure - Annually

        1)      At least annually, the electric fire pump and the diesel  fire  pump shall be flowed to
               determine if each is capable of flowing 100 gpm at the two highest outlets at a nozzle
               pressure  of 50  psi.  Single  50 foot lengths  of hose and 1/2 inch diameter nozzles
               should be used.

        2)      At least  annually, each length of fire  hose  should be  hydrostatically  tested to  the
               maximum pressure it is subjected to but not less than 100 psi.

        3)      At least annually, the fire  main should be flushed by flowing each outlet  for at least
               five minutes or  until clean  water appears.

        4)      At least annually, each valve in the fire main should be  cycled through  a complete
               open-close cycle.  The valves should be returned to their correct position.

-------
CHECKLIST

FIRE PUMPS

Pump Tested:

Electric Fire Pump:

Diesel Fire Pump:

Bilge Pump:

Start Time:
Stop Time:
Started from engine room:

Noise/Vibration/Leakage: _

Comments:
                                        SECTION: APPENDIX V, ATTACHMENT
                                        VERSION:  FINAL/MAY 1997
                                        PAGE: 2 of 4
Pressure at gauge:
                    Bridge:
Inspected By:
             Date:

-------
                                         SECTION: APPENDIX V, ATTACHMENT 1.
                                         VERSION:  FINAL/MAY  1997
                                         PAGE: 3 of 4
                                 R/V LAKE GUARDIAN
                                FIRE MAIN CHECKLIST
              Electric fire pump sea chest valve open

              Electric fire pump discharge valve open and locked

              Electric bilge pump discharge valve closed

              Fire main isolation valve at forward bulkhead open

              Diesel fire pump at sea chest valve open

              Diesel fire pump discharge valve open
Comments:
Inspected By:	        Date:_

-------
                                         SECTION: APPENDIX V, ATTACHMENT
                                         VERSION: FINAL/MAY 1997
                                         PAGE: 4 of 4

FIRE HOSE STATIONS

Hose Station Number         123456789   10   11   12

1) Gate valve closed:

2) Gate valve handle present:
I
3) Two gaskets present and in
   good condition:

4) Hose in good condition:

5) Spanner Wrench Present:

6) Nozzle present; handle free:

7) No visible leakage:

              All OK

Comments:
Inspected By: _ Date:
                            LOCATION OF FIRE STATIONS

1.      O-2 DECK Aft of Pilot House
2.      FOCSLE DECK forward port side
3.      O-l Deck midship port side
4.      Hotel Deck outside Captain's Office
5.      MAIN DECK aisleway port side outside Laundry Room
6.      MAIN DECK aisle stbd outside of Productivity LAB
7.      Science STORE ROOM
8.      FANTAIL stbd
9.      FANTAIL port
10.    Engine Room main aisleway port side outside machine shop
11.    ENGINE ROOM forward bulkhead
12.    ENGINE ROOM port side forward

-------
                                            SECTION: APPENDIX V, ATTACHMENT 1.9
                                            VERSION:  FINAL/MAY 1997
                                            Att.  1.9 - Pasie 1
Attachment 1.9 -  ENGINE ROOM CARBON DIOXIDE EXTINGUISHING SYSTEM
Procedure - Monthly

The  engine room carbon dioxide system  shall be inspected  monthly, or at approximately 30-day
intervals.   Extension  of the  schedule up to one week is permitted  in unusual circumstances.   The
inspection should include the  following:

        1)      Six cylinders  should be present,  connected to the manifold, and securely mounted.

        2)      There  should  be two control cylinders, two control valves, and discharge delay that are
               sealed in position with locking pins and tamper indicators.

        3)      The two system  release handles are in  place, with  "Break Glass" and  operating
               instructions present; two  brass hammers present.

        4)      The manual  pull  cables and conduits between  the  releases are  in good  operating
               condition and are connected to the valve operators.

        5)      The two emergency air  breathing apparatus are in the engine room and their gauges
               are in  the operable  range.

        6)      Four system nozzles in the engine room are not damaged, clogged or obstructed.

When any  deficient condition is found,  it shall  be noted on the checklist, and steps shall be taken to
correct  the deficiency.   The person performing  the  inspection  should complete, sign and  date  the
attached checklist.
Procedure - Annually

The engine room carbon dioxide system must be inspected and serviced by a qualified technician  The
inspection should include those items specified by NFPA  12.  The inspection should include a weight
check of the  cylinders.  The cylinders should be recharged  if a weight loss of more than 10%  of the
marked agent weight occurs.

-------
                                           SECTION: APPENDIX V, ATTACHMENT 1.9
                                           VERSION: FINAL/MAY  1997
                                           Alt. 1.9 - Page 2
CHECKLIST - ENGINE ROOM CARBON DIOXIDE SYSTEM
Six cylinders are present, connected to the manifold, and securely mounted:
No visible signs of damage:
Two control cylinders, two control valves, and one discharge delay are sealed in position with locking
pins and tamper indicators:	

Two  system  release  handles  are  in  place,  with   "Break   Glass"  and  operating  instructions
present:	

Two brass hammers present:	
The  manual  pull  cables  and  their  conduits  from  the  releases  to  the valve  operators  are
connected:	

No visible sign of damage:	
Two emergency air breathing apparatus are located in the engine room:_

The air breathing apparatus pressure gauges are in operable range:	
Four system nozzles in the engine room are not damaged, clogged or obstructed:_
Comments:
Inspected By:	    Date:

-------
                                     SECTION: APPENDIX V, ATTACHMENT 1.10
                                     VERSION: FINAL/MAY 1997
                                     Att. 1.10 - Page 1

ATTACHMENT 1.10 - R/V LAKE GUARDIAN FIRE ALARM CHECK LIST

A semi-annual test of the fire alarm system is required.

One sixth of all smoke  detectors must be inspected monthly.  Each monthly inspection will
comprise a different group of detectors insuring that each detector is inspected semi-annually.

      • 1 smoke detector for the navigating bridge (Pilot House)

      • 16  smoke detectors located on the 01 deck as follows:
      Y     N
      Q     Q     Room 201
      Q     Q     Room 202
      Q     Q     Room 203
      Q     Q     Room 204
      Q     Q     Forward passageway
      Q     Q     Room 207
      Q     Q     Room 208
      Q     Q     Room 209
      Q     Q     Room 210
      Q     a     Room 211
      Q     Q     Room 212
      Q     Q     After passageway
      Q     Q     Room 213
      Q     Q     Room 214
      Q     Q     Room 215
      Q     Q     Room 216

      • 8 smoke detectors on the main deck as follows:

      Y     N
      Q     Q     Crew's Lounge
      Q     Q     Galley
      Q     Q     Forward passageway
      Q     Q     After passageway
      Q     Q     Chem Lab
      Q     Q     Bio Lab
      Q     Q     Room 111
      Q     Q     Wet Lab -  1
      Q     Q     Rm. 3 SCI  (Forward of Bio-Lab)
      Q     Q     Crews Rm  (Forward of Lab Office)

-------
                                 SECTION: APPENDIX V, ATTACHMENT 1.10
                                 VERSION:  FINAL/MAY 1997
                                 Alt. 1.10- Page 2

Y     N
Q     Q     • Organic  Extraction Laboratory -  2 smoke detectors, 1 manual pull
             station

Q     Q       Organic  Analytical Laboratory -  2 smoke detectors, 1 manual pull
             station

Q     Q       Primary Productivity Lab - 2 smoke detectors, 1 manual pull station

Q     Q     • Hazmat Van - 3 smoke detectors, 1 manual pull station

Q     Q     • 1  smoke detector for the storage room( by the exercise room)

Q     Q     • 1  smoke detector for the exercise room

Q     Q     • 2  heat detectors for the port and starboard exhaust uptakes

Q     Q     • 1  heat detector for the bow thruster compartment

Q     Q     • 1  smoke detector for the MSD compartment

Q     Q     • 1  heat detector for the engineer's shop

Q     Q     • 1  heat detector for the auxiliary machinery compartment

Q     Q     • 1  heat detector for the engine room

Q     Q     • 1  heat detector for the forward engine room passageway

Q     Q     • 1  heat detector for the engine room aft passageway

Q     Q     • Carbon dioxide system pressure switch for the engine room
Comments/Discrepancies
                           Signature
                           Date

-------
                                                SECTION: APPENDIX V, ATTACHMENT 2
                                                VERSION:  FINAL/MAY 1997
                                                Att. 2 - Page 1

Attachment 2
R/V LAKE GUARDIAN - GENERAL LABORATORY WEEKLY INSPECTION CHECKLIST

A check mark indicates inspection met GLNPO standards. If conditions are unacceptable, indicate
date corrected or make comment below.

                                  LAB:  Bio    Chem Wet   Extr   Ana.   PP    Haz

1)     Eyewash working properly           	   	   	   	   	   	  	

2)     Hoods uncluttered                   	   	   	   	   	   	  	

3)     Containers properly labeled           	   	   	   	   	   	  	

4)     Hoods used  at proper sash height      	   	   	   	   	   	  	

5)     Waste containers properly labeled      	   	   	   	   	   	  	

6)     Chemicals kept in secondary
       containers                         	   	   	   	   	   	  	
7)     Chems stored according to
       compatibility

8)     Combustibles stored away from
       heaters

9)     GFCIs functioning properly

10)    Access to spill, first aid and other
       emergency equipment unobstructed
11)    Power cords in good working
       condition/free  from visible damage

12)    Compressed cylinders secured

13)    Exits unobstructed

Comments:
Inspected by:	      Date:

-------
                                                 SECTION: APPENDIX V, ATTACHMENT 3
                                                 VERSION:  FINAL/MAY  1997
                                                 PAGE: 1 of 4
ATTACHMENT 3 - VESSEL SANITATION
1.0 SCOPE

To provide references and procedures  to be used to ensure the proper sanitation for the vessel  and
crew.
2.0 REFERENCES

       U.S. Public Health Publications
       U.S. Navy Potable Specifications


3.0 RESPONSIBILITY - QUALIFIED PERSONNEL

       A.     The Chief Engineer or Marine Tech, under the authority of the Master, will undertake
              to fill and test, with the proper test chemical kits, the ship's potable
              water tanks under Section #7 below.

       B.     The cook, under the authority of the Master, will be responsible to store, prepare,  and
              cook all foodstuffs and dispose of all garbage aboard and adhere to daily and weekly
              inspections as listed below.


4.0 EQUIPMENT  REQUIRED

       • Potable water test kits
       • Potable water hoses and fittings
       • Sewage hoses and fittings
       • Rat guards for mooring lines (should be inspected by First Mate)
       • Temperature gauges for freezers and refrigerators
       • Fixed and hand-held appliances

5.0 ANNUAL INSPECTION

The  Master  shall  arrange for  this inspection  by a qualified public  health  person  or U.S.  Navy
counterpart, and the certificate  of acceptance is to be kept on board with copies sent to the EPA  and
the government contractor.

-------
                                                   SECTION: APPENDIX V, ATTACHMENT 3
                                                   VERSION:  FINAL/MAY 1997
                                                   PAGE: 2 of 4
6.0  PERIODIC INSPECTIONS

     A.   For all refrigerators and freezers in use:

         1.   Mount a temperature chart on the face of each door.

         2.   Record the temperatures twice daily.

         3.   Notify the Chief Engineer if temperatures are above 42 DF in the
             refrigerators  or above 10°F in the freezers.

     B.   Automatic Dishwasher:

         1.   Daily check  water temperature at 180°F minimum.

         2.   Remove daily or more often any debris in bottom of dishwasher.

         3.   Use caution  when opening door when dishwasher is  in "Dry"  cycle.  Hot steam can
             cause bums.

     C.   Trash Compactor:

         1.   Make sure that proper size bags are being used and installed properly.

         2.   Do not place pressurized cans or glass in the compactor (injuries
             could result).

         3.   Close door securely before turning key or pressing start.

         4.   If compactor will  not function electrically, notify Chief Engineer.
     D.  All appliances used in the galley department must be checked during
         each use to ensure safe operation.

     E.  Chief Engineer will test drinking water daily for coliforms and FACs.  Suggested kit -
         Colilert ONPG MUG Presence/Absence kit.

          1.  Test different locations each day:

             a. galley
             b. drinking fountains
             *Note on inspection log time and location.

-------
                                                   SECTION: APPENDIX V, ATTACHMENT 3
                                                   VERSION: FINAL/MAY  1997
                                                   PAGE: 3 of 4

         2.  Periodically test all remote faucets and sinks where potable water is
             obtainable.

         3.  Any outlet which fails to test should be logged and tagged "Do Not Drink".

         4.  Notify Master who will investigate the cause and determine  if
             more of the system is contaminated, and if so, take corrective action immediately.

7.0 POTABLE WATER

       A.     Chief Engineer will comply with Section 3.A of this attachment

       B.     Use only designated potable hoses.

       C.     Disinfect hose fittings and valve openings at both ends.

       D.     Inspect all hoses under pressure for leaks.  If any found, replace damaged section.

       E.     Maintain vessel trim while filling.

       F.     Notify bridge when pumping commences and is secured; advise quantity taken aboard.

       G.     Drain all hoses, cap open ends, store equipment in Potable H20 Storage Trunk.


8.0 SANITARY DISCHARGE

       A.     Equip work  party with protective  boots, gloves, suitable coveralls and  VHP portable
              radios.

       B.     Place containment pan  under discharge valve to contain  dripping during connecting
              and disconnecting procedure.

       C.     Stretch hoses to either  truck, sewer  opening or fixed  station, then  connect lines
              insuring no kinks or bends.  This could result in a burst hose.

       D.     Before  pumping is complete, it may be necessary to  flush all toilets to ensure that no
              lines are backed up if the holding tank was at capacity before pump out.

       E.     Keep bridge apprised of pumping start and stop times when using septic trucks hauling
              two or three  loads.

       F.     Upon  completion, close  discharge valves, flush lines, coil hoses and stow  in proper
              rack or reel.

-------
                                                  SECTION: APPENDIX V, ATTACHMENT 3
                                                  VERSION:  FINAL/MAY 1997
                                                  PAGE: 4 of 4
       G.     Remove  containment  pan, wash down  and hose off deck,  dock and  any protective
              clothing as necessary before stowing.

       H.     Return VHP radios to bridge.

       I.      Chief Engineer to trim vessel and advise Master when operation is complete and log is
              signed on.
9.0 REFRIGERATION

       A.     Notify Chief Engineer  immediately of any malfunction with freezers, refrigerators or
              ice machine.

       B.     Keep temperature logs as outlined under Section 6.0.A of this attachment.

       C.     Food to be rotated to prevent freezer burn.

       D.     Any spilled or spoiled food is to be removed and, if necessary, the unit defrosted and
              thoroughly cleaned before returning it to service.

-------
                       STANDARD OPERATING PROCEDURE
                      EMERGENCY SHOWER INSPECTION
Section




1.0




2.0




3.0




4.0




5.0




6.0




7.0
  Table of Contents






Title




Scope




References




Responsibility




Installation




Maintenance




Inspection




Operation
Page




2




2




2




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3




3




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Approved by:






Rev.
                  Date
                  Date
                                     Page 1

-------
                              Emergency Shower Inspection
1.0
       To  provide standard  operating procedures  for the safe use  and proper installation,
       maintenance and inspection of the emergency shower equipment.

2.0    References

       Haws Emergency Equipment Catalog
       GLNPO Chemical Hygiene Plan and Safety Manual

3.0    Responsibility - Qualified Personnel

       A.     The ship's Science Officer, Marine Science Technician,  Chemical Hygiene Officer or
              their appointed designee, under the authority of the Master, will be responsible for
              routine  maintenance and weekly testing of the emergency shower unit using the
              appropriate test kit as well as keeping accurate records of the data produced.

       B.     It shall be the responsibility of each specified authority to ensure that the flow of
              water required is in fact available as a result of regular system inspections.

4.0    Installation

       A.     Units should be installed in  close proximity to hazardous areas, clearly identified as
              emergency shower stations, free from obstructions and accessible within 10 feet of at
              least one exit from each laboratory.

       B.     Emergency showers should  be connected to the main potable water supply.

       C.     Minimum pipe size to shower should be no smaller than 1 and 1/4" IPS.
                                         Page 2

-------
5.0    Maintenance
       A.     Water pressure at the shower should be no less than 30 PSI or more than 90 PSI
              during operation.
6.0    Inspection
       A.     All emergency shower units should be tested weekly.  Since there is no floor drain
              available, a Haws Shower Test Kit, Model 9010, should be used.

       B.     Accurate records should be kept that indicate that adequate flow exists with absence
              of sediment. The date of the inspection, name of the inspector and details regarding
              the state of the equipment will be included.  (See attached shower inspection chart).
7.0    Operation
       A.     To insure adequate operation of the units, all persons should be instructed in the
              proper use of the emergency shower equipment.

       B.     The injured person should immediately make use of the facility by showering and
              removing all clothing as
              quickly as possible.

       C.     Any emergency use of the safety shower should immediately be reported to the Bridge
              and recorded in the ship's log.
                                         Page 3

-------
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                       STANDARD OPERATING PROCEDURES
                             EYE WASH INSPECTION
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2.0




3.0




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6.0




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  Table of Contents







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Scope




References




Responsibility




Installation




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Inspection




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-------
                                    Eve Wash Inspection
1.0    Scope
       To provide standard operating procedures for the safe use and proper installation,
       maintenance and inspection of the emergency eye wash equipment.

2.0    References

       Haws Emergency Equipment Catalog
       GLNPO Chemical Hygiene Plan and Safety Manual

3.0    Responsibility - Qualified Personnel

       A.     The ship's Science Officer, Marine Science Technician and/or the Chemical Hygiene
              Officer  or  their appointed  designee, under the authority of the Master, will be
              responsible for routine maintenance and weekly testing of the emergency eye wash
              unit as well as keeping accurate records of the data produced.

       B.     It shall be the responsibility of each specified authority to ensure that the flow of
              water required is in fact available as a result of regular system inspections.

4.0    Installation

       A.     Units should be installed in close proximity to hazardous areas, clearly identified as
              eye wash stations, free from obstructions and easily accessible.

       B.     Eye washes should be connected to the main potable water
              supply.

       C.     Eye wash fountains should be connected with piping no smaller than 3/4" IPS.  All
              eye wash units should be attached to a drain by a code approved method to facilitate
              ease of testing.
                                          Page 2

-------
5.0    Maintenance
       A.     Water pressure at the eye-wash fountain should be no less than 30 PSI or more than
              90 PSI during operation.

       B.     Because of sediment accumulation in  standing pipes,  flusHng  times  should be
              determined by the  Science  Officer, Marine Science  Technician or designated
              appointee prior to ship's departure.
6.0    Inspection
       A.     All emergency eye-wash units should be tested weekly.  The unit should be flushed
              for the required amount of time as determined above.

       B.     Accurate records should be kept  that indicate that adequate absence of sediment and
              proper eye dispersion rates are  being met.  The date of the inspection, name of the
              inspector and details regarding the state of the  equipment will be included.   (See
              attached eye wash inspection chart).
7.0    Operation
       A.     To insure adequate operation of the units, all persons should be instructed in the
              proper use of the emergency eye wash equipment.

       B.     Eyes should be continually rinsed at the scene of the accident for at least 15 minutes
              and a doctor or nurse contacted as  soon as possible.

       C.     Any emergency use of the eye wash unit must be immediately reported to the Bridge
              and recorded in the ship's log.
                                         Page 3

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-------
                                                      SECTION: APPENDIX V, ATTACHMENT 6
                                                      VERSION:  FINAL/MAY 1997
                                                      PAGE: 1 of 1


ATTACHMENT 6, MONTHLY INSPECTION FOR SELF-CONTAINED BREATHING APPARATUS

SCBA Number and Location	

COMPLETENESS

       Ensure that  all  required components are available:  facepiece,  breathing tube assembly, regulator, hose
       assembly, cylinder, valve assembly, harness, back-plate assembly complete with all straps.

CYLINDER PRESSURE

       Ensure cylinder is charged to 2216 psi and that cylinder is within hydrostatic test date.

CONDITION CHECK

       A.     Visually check for any signs of wear, deterioration, wrinkling, or broken parts; check facepiece,
              valves, and breathing tube assembly.

       B.     Check facepiece and breathing tube for elasticity and cracks

       C.     Check head harness for wear and elasticity, ensure it is properly attached to face-piece

       D.     Check regulator for breakage or missing screws

       E.     Check cylinder and valve assembly for deep scratches dents, heat exposure, or other deformities

       F.     Examine backframe and harness for signs of wear

LEAK CHECK

       Check Breathing System:
         open valve fully, hold breath and place face-piece against face, close cylinder valve and observe remote
         gauge.   If pressure decreases this indicates a leaks.  If there is a leak, use soap;  solution to isolate
         affected area.

       Negative Pressure Leak Test:
         close main valve, gently  inhale until the air in system is  gone and a negative pressure should  be
         maintained with  no leakage through the regulator

ALARM CHECK

       Place face-piece to face, turn cylinder fully on, turn  cylinder off,  watch gauge while  breathing slowly,
       alarm should sound when the regulator gauge indicates 1/4 remaining service life:

DISINFECTION

       Disinfect SCBA with safety cleaning equipment after use

REPORT ANY DEFICIENCIES TO THE SAFETY EQUIPMENT MANAGER

Notes:  	


Inspected By:	    Date:	

-------
                                             SECTION:  APPENDIX V, ATTACHMENT 7
                                             VERSION:  FINAL/MAY 1997
                                             PAGE: 1 of 1
Attachment 7 - HAZARDOUS WASTE STORAGE AREA INSPECTION

Location  _
Q     Q     Are the containers in good condition (no leaks, rusting containers, or bulges)?
Q     Q     Is the storage area free of debris?
Q     Q     Are the containers properly contained (e.g., in a diked area)?
Q     Q     Is the floor dry (no sign of spills)?
Q     Q     Are the containers properly labeled, including start accumulation date?
Q     Q     Are the inorganic containers interlined with polyethylene?
Q     Q     Are the containers closed?
Q     Q     Are containers holding incompatible  wastes  kept  apart  by  physical barrier  or
              sufficient distance?
Q     Q     Is spill cart adequately stocked (see spill  cart inspection form)?

If any of the above were answered no, please provide explanation:
1.      Number of waste containers from dissolved oxygen analytical process:	
       Total amount in gallons:	
2.      Number of Green Label Flammable (<50 ppm PCBs) Hazardous Waste Containers:
       Total amount in gallons:	
3.      Number of Yellow Label Acid Hazardous Waste Containers:	
       Total amount in gallons:	
4.      Number of acetone/water rinse water waste containers:	
       Total amount in gallons:	
5.      Number of Radioactive Waste Containers:	
       Total amount in gallons/activity:	
6.      Other miscellaneous waste streams:	
       Total amount in gallons:	
       Oldest accumulation start date:_
       Most recent shipment by date	
Inspected By                         Date          Reviewed By                  Date

-------
                                                     SECTION: APPENDIX V, ATTACHMENT
                                                     VERSION:  FINAL/MAY 1997
                                                     PAGE: 1 of 1
Attachment 8 - SPILL CART CHECKLIST FORM
Checked By:
               QUANTITY
DESCRIPTION
               30
               50
               20
               4kg
               l.lkg
               3.2kg
               1.2kg
               1
               6
               1
               1
               1
               1
               1
               1
               I roll
               1
               1
               1
               1 roll
               1 roll
               1
               1 each
               1
               1
               6
               1
               1
               1
               1
               2
               1
               1 pair
                 pair
Universal Spill Control Pillows™, 1 liter size
Hazardous Material Disposal Bags,  12"xl8"
VBS Blankets™
Hg Absorb™ Powder
Resisorb™
Neutrasorb® Acid Neutralizer
Neutracit® Caustic Neutralizer
Chemop™
Chemsponges™
Spill Squeegee, floor size, 18" head
Spill Squeegee, bench size, 8" head
24 oz. Mophead & handle
Polypropylene Broom
Bench Brush
Dust Pan
KIMTEX ™ Towels, 9 3/4"x 100 ft. roll
Sponge, cellulose
Liquid Cleaner, 32 oz.
Clorox,  1 gallon
pH Paper
Barricade tape, 100 ft.
Sealing Tape for clothing containers, 180 ft.
Safety Flashlight & Power Shell Battery Pack
Tongs, 20" long
SpillKART™ with rigid liner
SpillKART™ Liners, yellow, 36" x 48"
Mop Bucket, 35 qt.
Wringer
Floor Sign
Glass Disposal Box, 8" x 8" x 10"
Chemical Spill Clothing Kits, each kit includes:
Total Body Coverall, polylaminated Tyvek
Nitrile Gloves
Disposable Polyethylene Gloves
Chemical Splash Goggles, fog-free lens
Respirator with dust, acid gas/organic vapor cartridges
Hydrogen Fluoride Respirator
Toxic Dust Respirator
Date:

-------
                                                SECTION: APPENDIX V, ATTACHMENT 9
                                                VERSION:  FINAL/MAY 1997
                                                PAGE: 1 of 2
Attachment 9 - GLNPO SPECIFICATIONS FOR DRY-DOCKING AND
              OVERHAUL INSPECTIONS
The following items must be included in a five year dry-docking and overhaul inspection:

       1.  Dry Docking

       2.  Services
              a.  Docking Services
              b.  Shore Power
              c.  Gangway
              d.  Fire Hose
              e.  Waste Removal:  Unclassed/Hazardous
              f.  Office Space

       3.  Hull Inspection
              a.  Bottom Plate

       4.  Zinc Anodes
              a.  Zinc Restoration

       5.  Engine Alignment

       6.  Propellers
              a.  Propeller Inspection
              b.  Propeller Restoration

       7.  Propeller Shafts/Bearing

       8.  Rudder

       9.  Sea Chests/Overboard Values

       10.  Bottom Paint

       11.  Vents

       12.  Water Tight Doors/Hatches

       13.  Transducer

       14.  Anchors/Chains

       15.  Inclining

-------
                                         SECTION: APPENDIX V, ATTACHMENT
                                         VERSION:  FINAL/MAY  1997
                                         PAGE: 2 of 2
16.  Electrical

17.  Alarm System
       a. Tank Level Alarm System

18.  Pumps
       a.  Hydraulic
       b.  Fire
       c.  Bilge
       d.  Repairs

19.  Engines
       a.  Mains
       b.  Generators

20.  Safety Equipment
       a.  Rafts
       b.  Extinguishers
       c.  Fire Suppression Systems

21.  Navigation Equipment
       a.  Auto Pilot
       b. Gyroscope
       c. Radars
       d. LORAN

22.  Waste Holding Tanks

23.  Labor Rates

-------
                                          SECTION: APPENDIX V, ATTACHMENT
                                          VERSION:  FINAL/MAY 1997
Attachment - R/V LAKE GUARDIAN - GENERAL WEEKLY INSPECTION CHECKLIST

	  Emergency lighting functional

	  Emergency exits unobstructed

	  Fire doors closed

	  Tool room equipment clean and free of accumulated debris and shavings

	  Machine guards in  place on tool room equipment

	  Dielectric mat placed in front of main control panel in Engine Room

	  Circuit boxes in safe condition and doors are closed

	  Compressed cylinders properly secured

	  All GFCIs are functional

	  All extension cords are grounded,  in good condition, and not in permanent  use

	  No combustibles stored in exhaust trunks (port or starboard)

	  Paint locker in good order with no open containers, and amount of paint is within allowable
         limits

	  Freezer and refrigerator panic bars are operational

	  Door closers functioning properly
Comments:
Inspected by:	       Date:

-------
                       R/V LAKE GUARDIAN
                       PRE-SAIL CHECKLIST
SHIP-BOARD CHECKLIST

	  ENSURE MAGNETIC DOOR CLOSURES ARE DEACTIVATED

	  ORIENTATION AND PAPERWORK COMPLETED FOR ALL NEWLY ARRIVED
     PERSONS

	  DECKS SECURED

	  SAMPLING EQUIPMENT SECURED

	  ENSURE RESTRICTED AREAS ARE SECURED (TAPED OFF WITH CAUTION TAPE)

BRIDGE CHECKLIST

	  NAVIGATIONAL LIGHTS FUNCTIONAL

	  TASK LIGHTS FUNCTIONAL

	  GYRO STEERING ALARM FUNCTIONAL

	  FURUNO FATHOMETER FUNCTIONAL

	  ELECTRONIC HAILER FUNCTIONAL

	  PORTABLE HAILER AVAILABLE AND FUNCTIONAL

	  LORAN LC-90 FUNCTIONAL

	  LORAN NORTHSTAR FUNCTIONAL

	  RAY-88 VHF FUNCTIONAL

	  STANDARD VHF RADIOS AVAILABLE AND CHARGED

	  CLOCKS SET AND FUNCTIONAL

	  RECORDING BAROMETER FUNCTIONAL

     WALL BAROMETER FUNCTIONAL

-------
Pre-Sail Check
Page
     THERMOMETERS (2) AVAILABLE AND FUNCTIONAL


     GENERAL ALARM FUNCTIONAL

     CO2 AL \RM FUNCTIONAL

     HOSE-McCANN ENGINE ROOM PHONE FUNCTIONAL

     DECCA RADAR (LARGE) FUNCTIONAL


     DECCA RADAR (SMALL) FUNCTIONAL


     NAVIGATIONAL INSTRUMENTS FUNCTIONAL

     PILOT RULES AVAILABLE

     NOTICE TO MARINERS PUBLICATIONS CURRENT


     LIGHT LIST AVAILABLE


     COAST PILOT


     NAVIGATIONAL CHARTS  AVAILABLE

     LOG BOOK AVAILABLE

     NIGHT ORDER BOOK AVAILABLE

     RADIO LOG AVAILABLE

     FAN-ENGINE CONSOLE LIGHTS FUNCTIONAL


     SEARCH LIGHTS FUNCTIONAL

     INTERCOM FUNCTIONAL

     HYDRAULIC STEERING FUNCTIONAL


     MAGNETIC COMPASS LIGHTS FUNCTIONAL AND FULL OF OIL


     DAY SHAPES  AVAILABLE

-------
Pre-Sail Check
Page

	 GYRO REPEATER LIGHTS FUNCTIONAL


	 DESK LAMP FUNCTIONAL


	 FURUNO DEPTH RECORDER PAPER AVAILABLE

	 SINGLE SIDE BAND RADIO FUNCTIONAL

	 12 VOLT BATTERIES (AFT OF PILOT HOUSE) FUNCTIONAL


	 FIRST AID KIT STOCKED AND AVAILABLE


	 BINOCULARS AVAILABLE (3)


	 WINDSHIELD WIPERS (FORWARD) FUNCTIONAL

	 WINDSHIELD WIPERS (AFT) FUNCTIONAL

	 SHIP'S BELL FUNCTIONAL


	 INSIGNIAS AVAILABLE : BRAVO, EPA, SSI, CAPTAIN ISMA


	 GUEST BOOK AVAILABLE


	 WORK VESTS AVAILABLE

	 EPIRB AVAILABLE AND FUNCTIONAL

	 FOG SIGNAL (AUTO) FUNCTIONAL

	 SURVIVAL SUITS AVAILABLE


	 EMERGENCY FLARES AVAILABLE (CHECK EXPIRATION DATE)


	 VERY PISTOL AVAILABLE


	 LINE THROWING DEVICE AVAILABLE

	 PORTABLE COMPASS AVAILABLE


     ALIDADE AVAILABLE AND FUNCTIONAL

-------
Pre-Sail Check
Page
     NAVIGATIONAL TRIANGLES AVAILABLE

     SPARE BULBS AVAILABLE FOR EQUIPMENT

     STATION BELL UPDATED AND POSTED (ENSURING CREW MEMBERS ARE
     AWARE OF THEIR RESPONSIBILITIES, AND ARE CAPABLE OF PERFORMANCE)

-------
                          NOTICE
This boat is not to be put into operation until necessary equipment
is installed and a pre-deployment  safety  inspection is conducted.

The  following  will  be  included  in  the  pre-deployment  safety
inspection:

     	1.    Sufficient Number  of  Personal  Flotation  Devices
               (PFD)
     	2.    Sufficient Fire Extinguisher
     	3.    Proper Loading  of  Equipment  (No  overloading  of
               vessel)
     	4.    Proper Navigation Lighting
         .5.    Check  For  Fuel Leaks; Condition of Fuel line
         _6.    Sound  Producing Device Available
         Jl.    Visual Distress Signals, if appropriate (Ex. night
               travel or travelling in waters  greater than 2 miles
               in  width)
         .8.    Paddles or  Oars
         _9.    Anchor and  Line
          10.   Dewatering  Device (Pump, Bucket, or Scoop)
         ,11.   Condition  of Wiring
         .12.   First  Aid Kit
         _13.   Radio
          14.   Tool Kit (optional)
         _15.   Fender (optional)
         _16.   Perform a General Condition Inspection


                                     Bv,:	

-------
U.S. Environmental Protection Agency

77 West Jackson Boulevard, 12th Floor
Chicago, IL  60604-3590

-------