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VIII. OPERATIONAL PROCEDURES AND DATA
A. PROCEDURES FOR RECEIVING DAILY REFUSE
Twenty-two city and five county refuse trucks began their
deliveries at approximately 7 a.m. each day and continued until
5:00 p.m. to 5:30 p.m. except on Wednesdays and Saturdays. On
Wednesday six city commercial trucks were used and on Saturday
usually about three were used. Usually the county did not de-
liver on Saturdays. The University of Florida used two trucks
to deliver one through three loads each day including Saturday.
The following compilation describes the trucks and wastes.
TRUCK NUMBER KIND OF SIZE OF REFUSE
OWNERSHIP OF TRUCKS TRUCKS TRUCKS TYPE
AVERAGE
WASTE DENSITY
lb/yd3
City
City
City
City
City
University
University
County
County
1
3
2
1
15
1
1
4
1
Dump Meter
Dump Meter
Dump Meter
Dump Meter
Rear Loader
Compaction
Compaction
Compaction
Compaction
Open Dump
15 yd.
20 yd.
30 yd.
20 yd.
20 yd.
40 yd.
65 yd.
-
-
Commercial , Industrial
and apartments
Commercial, Industrial
and apartments
Commercial, Industrial
and apartments
Mixed Domestic
Mixed Domestic
Mixed Domestic and
Hospital
Mixed Domestic and
Hospital
Mixed Domestic
Mixed Domestic
322
322
322
322
322
378
378
-
-
B. MATERIALS DATA
The number of trucks and quantity of refuse received on a
monthly basis is shown in Table A for the years of 1968 and 1969.
The quantity of salvaged materials, non-compostables, and compost
produced is shown in Table B.
-84-
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IX. OPERATING COSTS
The operating costs presented in this report include only
the actual cost of operation. They do not include depreciation.
Because of the prolonged shakedown and the inefficient operation
of the primary grinder during the first year of operation (1968)
costs are given only for the second year (1969). Costs are
divided into various categories representing the steps in the
total process. Table A shows the monthly total costs and cost per
ton of processed refuse for the various categories of the total
process during the calendar year of 1969. Table B shows the
operating costs of major pieces of equipment.
The "General Administration" category consists of office
expense including salaries of Project Director and Clerk-Secretary.
Consultants fees are not included as these would not be a part of
the operating costs of a conventional compost plant.
-87-
-------
TABLE A
OPERATING COSTS - DEPARTMENTS
DSPAJRTWZNT
DELIVERY & RECEIVING:
Tons Processed
Total Cost
Cost/Ton
SALVAGES
Tons Processed
1 Total Co:st
1 Cost/Ton
!
1 GRINDING:
i Tons Processed j
i Total Coat
I Cost/Ton
IKON-COMPOS TABLES:
\ Tons Processed
Total Cost
Cost/ Ton
,
! DIGEST I NG &
1 SLUMS HA.VDLI.VGj
jj Tons Processed
Total Cost
} Gos t/Ton
JFINXSHING: (Rewind insr)
| Tons Processed
Total Cost
Cos t/Ton
YARDS & STORAGES
| Total Cost
F
GENERAL ADM IN IS TRAT TON :
f Total Cost
t
! GENERAL PLANT -
j SUPERVISION •
] Total Cost
jj
! TOTAL OPERATING COST
i Cost/Ton
JANUARY
2,972.4
$ 1,111.26
.38
225.5
$ 1,799.46
7.90
2,233.5
$ 1,777.15
.80
662.0
I 3,581.07
5.40
2,084.9
$ 995.54
.48
1,561.1
$ 1,799.92
1.15
$ 426.30
$ 2,551.32
$ 6,966.42
$21,008.94
7.07
FEBRUARY
2,723.2
1 705.42
.26
215.1
$1,603.29
7.45
2,023.9
1 3,307.02
1.64
620.3
$ 3,260.86
5.21
1,887.8
$1,317.48
.70
1,347.5
$ 1,790.25
1.33
$ 283.74
$2,706.51
$ 7,515.60
$22,490.16
8.26
MARCH
2,917.6
$ 1,382.81
,65
185.0
$ 1,575.14
8.51
2,306.8
$3,383.60
1.47
571.1
$ 3,121.22
5.47
2,161.5
$ 1,726.74
.80
1.4//1.5
$ 2,840.20
1.97
$ 872.29
$ 3,431.13
$ 3,220.92
$?2,109.05
7.5*
APRIL
1,317.6
$ 1,538.89
1,17
173.1
$ 1 , 444 * *-' *
8.33
825.8
$ 2,917,33
3.53
384.4
$ 2,454.20
6.38
759.9
$ 1,040.69
1.37
907.9
$ 1,920.48
2.12
$ 948.79
$ 3,832.02
S 3,630.08
519,726.56
14.97
GENERAL PLANT SUPERVISION: Includes such items as social security costs,
workmen's compensation, group insurance, and general insurance.
A - 1
-88-
-------
TABLE A (can't)
OPERATING COSTS - DEPARTMENTS
DEPARTMENT
DELIVERY § RECEIVING:
Tons Processed
Total Cost
Cost/Ton
SALVAGE:
Tons Processed
Total Cost
Cost/Ton
GRINDING:
Tons Processed
Total Cost
Cost/Ton
WN-OOMPOSTABLES:
Tons Processed
Total Cost
Cost/Ton
HGESTING §
SLUDGE HANDLING
Tons Processed
Total Cost
Cost/Ton
:INISHING: (Regrinding)
Tons Processed
Total Cost
Cost/Ton
fARDS AND STORAGE:
Total Cost
JENERAL ADMINISTRATION:
Total Cost
JENERAL PLANT -
SUPERVISION:*
Total Cost
TOTAL OPERATING COST
Cost/Ton
MAY
2,042.0
$ 686.74
.34
175.5
$ 1,718.70
9.79
1,407.5
$ 2,953.52
2.10
458.9
$ 2,210.36
4.82
1,407.6
$ 1,308.60
.93
1,150.0
$ 1,565.27
1.36
$ 444.50
$ 2,538.11
\
$ 4,237.10
$ 17,762.90
8.70
JUNE**
1,023.1
$ 885.82
.87
76.5
. $ 1,128.77
14.75
730.2
$ 2,148.54
2.94
216.4
$ 1,943.72
8.98
730.2
$ 1,674.12
2.29
1,494.6
$ 1,344.92
.90
$ 902.95
$ 2,624.79
$ 4,504.69
$ 17,158.32
16.77
JULY
3,239.9
$ 1,104.39
.34
250.2
$ 2,309.93
9.23
2,658.9
$ 2,945.74
1.11
482.9
$ 2,069.63
4.29
2,506.8
$ 1,572.96
.63
1,500.0
$ 3,064.80
2.04
$ ' 651.33
$ 2,577.27
$ 5,152.73
$ 21,448.78
6.62
AUGUST
3,376.1
$ 1,323.83
.39
272.5
$ 1,753.19
6.43
2,604.8
$ 3,893.16
1.49
498.8
$ 3,330.54
6.68
2,604.8
$ 1,567.53
.60
1,613.2
$ 2,663.83
1.65
$ 628.81
$ 2,632.54
$ 4,323.89
$ 22,116.32
6.551
* Includes such items as social security costs, workmen's compensation, group
insurance and general insurance.
** High June costs due to low intake tonnage and shut-down to change over to
new primary grinder.
A - 2
-89-
-------
TABLE A (can't)
OPERATING COSTS - DEPARTMENTS
DEPARTMENT
DELIVERY § RECEIVING:
Tons Processed
Total Cost
Cost/Ton
SALVAGE:
Tons Processed
Total Cost
Cost/Ton
GRINDING:
Tons Processed
Total Cost
Cost/Ton
M3N-COMPOSTABLES:
Tons Processed
Toral Cost
j Cost/Ton
DIGESTING §
1 SLUDGE HANDLING:
Tons Processed
Total Cost
Cost/Ton
FINISHING: (Regrinding)
Tons Processed
Total Cost
Cost/Ton
YARDS AND STORAGE:
Total Cost
GENERAL ADMINISTRATION:
Total Cost
GENERAL PLANT
SUPERVISION:
Total Cost
TOTAL OPERATING COST
Cost/Ton
SEPTEMBER
4,067.7
$ 1,549.27
.38
238.1
$ 1,895.48
7.96
3,324.0
$ 3,606.20
1.08
505.6
$ 3,734.32
7.39
3,324.0
$ 1,231.12
.37
2,283.6
$ 2,842.34
1.24
$ 439.97
$ 2,051.54
$ 4,738.96
$ 22,089.20
5.43
OCTOBER
3,498.4
$ 1,839.17
.53
162.1
$ 1,403.61
8.66
2,833.9
$ 5,016.04
1.77
502.4
$ 2,874.01
5.72
2,833.9
$ 6,712.11
2.37
2,059.8
$ 3,634.04
1.76
$ 1,444.40
$ 3,080.46
$ 6,814.11
$ 32,817.95
9.38
NOVEMBER
3,003.4
$ 1,453.67
.48
147.1
$ 1,214.20
8.25
2,369.8
$ 5,743.60
2.42
486.5
$ 2,710.14
5.57
2,369.8
$ 2,707.10
1.14
3,176.0
$ 2,221.93
.70
$ 2,322.05
$ 2,278.01
$ 4,779.61
$25,430.31
8.47
DECEMBER
3,891.1
$ 1,885.45
.48
131.6
$ 1,476.51
11.22
3,193.9
$ 3,850.12
1.21
565.6
$ 3,494.55
6.18
3,193.9
$ 2,652.67
.83
4,264.1
$ 2,677.29
.63
$ 1,124.06
$ 3,179.72
$ 5,756.90
$26,097.27
6.70
A - 3
-90-
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X. PAPER AND CCMPOST SALES
A. PAPER
Recycling and marketing of paper was a successful operation
during the first 1 2/3 years of plant operation. A lot of good
quality newsprint and corrugated paper has been received at the
plant and as a result a good market developed with the gypsum aind
roofing industry. Bales were made up with a mixture of newsprint
and corrugated with a higher percentage of the latter. This is
more desirable for the two industries mentioned due to the higher
content of long fibers. An average price of about $17.50 per ton
was received during this period. However, from September on to
the end of 1970, the market was depressed due to tight money and
a slow down in the construction industry. The last price re-
ceived was $19.00 per ton and tonnage purchased was cut back
considerably. Table A shows paper and compost sales during 1968
and 1969.
B. CCMPOST
Most of the compost sales have been in the citrus industry
in bulk form. This has not been an encouraging picture as only
10% of the compost produced has been sold. Citrus sales were made
primarily to 32 different grove managers, in amounts ranging from
13 tons to a high of 400 tons, with tonnage per acre varying from
1 1/2 to 38. The average application was about 3 tons per acre.
-92-
-------
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Transportation, no doubt, was a factor in limiting the amount of
compost sold. Hauling distances ranged from 90 to 170 miles from
the plant at a cost of from $14.00 to $16.00 per ton delivered
and spread on the groves. Of the total Customers, four repeated
applications in three consecutive years. Claims were made by these
growers that: 1) they had an improvement in the cover crop grown,
2) that the juice content of the product grown had increased, 3)
that the yield of fruit per acre had increased, and 4) that it took
less time for fruit to reach maturity.
A good percentage of the compost produced was donated to
the public participants, mainly the University of Florida, and some
to the City of Gainesville in the development of public parks.
The University has used the compost as a soil builder, mixing it
with sandy soil and amending it with nitrogen, to be used on the
ground where new building projects have been completed.
Other areas of compost application have been for pine tree
seedlings, truck gardening, and making potting soil blends. One
such application with pine tree seedlings was carried on with St.
Regis Paper Company in north Florida, where applications were
made in various quantities from 3 to 20 tons per acre. Their ex-
perience indicated that 10 tons to the acre gave them the best
results with the survival rate of seedlings in the test plots
exceeding those in the control plots.
-94-
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Much research needs to be carried on in developing markets
for compost. It is planned that as the plant continues to operate,
studies and experiments will be carried on where large quantities
of compost can be utilized. Two such projects that are planned for
Florida are land reclamation and organic fanning. Work is already
under way in which 100 acres of virtually sterile soil, due to a
strip mining operation, will be saturated with compost to try
and improve and make the soil productive again. The other project
is approximately 400 acres of unproductive, sandy farm soil which
will be applied with various tonnages of compost. The compost
will be plowed into the soil, left to decompose and crops planted
after a year. It is hoped that the results of these, tests will
develop a future market for compost produced. At the same time
the theory of total reutilization of solid waste will become a
reality.
-95-
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TECHNICAL EVALUATION
-96-
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TABLE OF CONTENTS
TECHNICAL EVALUATION
Page No.
A. INTRODUCTION 99
B. FACILITIES AM) EQUIPMENT 100
1. Laboratory 100
2. Process Control and Special Studies Equipment 103
3. Moisture Sensor 103
4. Temperature Sensor Probes 104
5. Mobile Instrument Console 105
C. METHODS OF SAMPLING 107
D. METHODS FOR CHEMICAL ANALYSES 108
1. Carbon 109
2. Nitrogen 110
3. Phosphorus 110
4. Potassium 111
5. Moisture 111
6. Hydrogen-Ion Concentration 112
7. Volatile Solids 112
8. Chemical Oxygen Demand 113
9. Biochemical Oxygen Demand 113
10. Putrescible Matter 115
E. BACTERIOLOGICAL ANALYSES 118
-97-
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E. BACTERIOLOGICAL ANALYSES
(Continued)
Page No.
1. Coliform Bacteria "
2. Salmonella 119
3. Extrinsic Bacteria 119
F. OCCUPATIONAL HEALTH STUDIES 123
1. Physical Examinations 124
2. Noise Levels 124
3. Intestinal Parasites 124
4. Airborne Particulate Matter 125
G. ARTHROPOD AND RODENT CONTROL METHODS 126
H. RESULTS 127
1. Composition of Refuse as Received at The Plant 127
2 . Compos ition of Material Removed by Magnets
3. Bulk Density of Refuse as Placed in Digester 133
4. Moisture Content of Refuse and Compost 134
5. Sewage Sludge Utilization 137
6. Effect of Sewage Sludge on Refuse Decomposition 145
7. Decomposition of Refuse in the Digester 153
8. Curing of Compost 172
9. Bulk Density of Compost in Storage Pile 190
10 . Process Evaluation - Miscellaneous 191
11. Arthropod and Rodent Control 196
12. Public Health Aspects of Composting 218
REFERENCES 237
-98-
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TECHNICAL EVALUATION
A. INTRODUCTION
The principal objective of the technical program of the
Gainesville Demonstration Project is to provide the technical sip-
port required to demonstrate the satisfactory performance of the
Metro composting system for the treatment of solid wastes from
medium-sized cities. The specific objectives are to:
1) determine what is accomplished by the composting
process; and
2) evaluate the public health aspects of the process.
When these objectives were achieved, recommendations in the form of
revised operating parameters were made to improve the process. If
the operating parameters were revised, their effect on improving the
process was subsequently evaluated. Data resulting from the fulfill-
ment of these objectives could be used by:
1) the U. S. Public Health Service to evaluate the
utility of composting as a treatment process to
partially alleviate the nation-wide solid waste
problem;
2) plant operators in the selection of process control
parameters to attain more efficient treatment; and
3) technical personnel in control and research work
on solid wastes.
-99-
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During the course of this demonstration project, the Metro
composting process, as an example of a high-rate composting system,
was evaluated for its utility in the treatment of solid municipal
wastes .
B. FACILITIES AND EQUIPMENT
The facilities for conducting the chemical and microbiological
studies associated with this evaluation program include a combination
chemistry and bacteriological laboratory and special in-plant equip-
ment for monitoring several of the factors which influence the compost-
ing process and the quality of the final product.
1. Laboratory
A combination chemistry-bacteriological laboratory is
located on site in the compost plant office building. This laboratory
has about 360 sq. ft. of floor space and is fully equipped for most of
the chemical analyses and all routine bacteriological studies necessary
for the demonstration and quality control program. Figure 1 shows a
floor plan of the laboratory and the location of the major pieces of
equipment. A description of the major laboratory equipment is pre-
sented in Table 1.
The staffing of the laboratory varied during the dura-
tion of the project. Initially, it was supervised by a part-time
professional biologist. He was assisted by a full-time technician
and three part-time graduate students, one each to work on the chemical,
-&0-
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Item
TABLE 1
MAJOR LABORATORY EQUIPMENT
Manufacturer
1. Drying Oven
2. Analytical blance
3. Water Still
4. Water bath
5. Steam Generator
6. Glassware washer
7. pH Meter
8. Kjeldahl Unit
9. Moisture Balance
10. Induction Furnace
11. Klett Industrial
Photometer
12. Autoclave
13. Incubators (2)
14. Mikro-Samplmill
15. BOD Incubator
16. Andersen Air Sampler
Pulverizing Machinery
Div. Metals Disinte-
grating Co., Inc.
Andersen Samplers and
Consulting Service
Model No.
Precision Thelco
Mettler
Barns te ad
Precision
Amsco
Heinicke
Sargent
Labconco
Ohaus
Leco
t
Klett
Scanlan Morris
Precision Thelco
27
H-15
EMH-5
66634
LB20
HW200S
PL (No. 30008)
20701
6010
521-000
900-3
A-422
4
-101-
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VENT
-102-
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bacteriological, and arthropod and rodent control aspects. Staffing
of the technical evaluation program was continually enlarged until
it included a full-time director who was a professional microbiolegist,
a full-time graduate engineer, a full-time technician, and two half-
time technicians.
2. Process Control and Special Studies Equipment
The compost plant is equipped so that the moisture
content of the ground raw refuse and the temperature of each batch
of compost in the digester tanks are continuously monitored. In
addition to the continuous monitoring equipment, a special mobile
instrument package was developed by Honeywell, Inc., to measure the
temperature, dew point, 0- content, and CO- content at several levels
anywhere in the digester tank, and also to measure the volume of air
entering a segment of the digester tank.
3. Moisture Sensor
The moisture content of the ground refuse was measured
as a function of the conductivity between two sensing electrodes in
an A.C. bridge circuit. The signal generated by this circuit is cali-
brated in terms of moisture content and is recorded on a circular
chart recorder.
The sensing electrodes consist of two metal shoes 1 1/4
inches wide and 4 1/4 inches on center. These sensors are on a pivoted
arm that rides on the surface of the ground refuse as it is conveyed
-103-
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from the mixing screws following the secondary grinder to the
digester tanks. The electric signal from these electrodes acti-
vates a variable speed pump which supplies either water or sewage
sludge to the refuse in the mixing screws in order to maintain a
pre-selected moisture content in the ground refuse. A limit switch
is connected in the circuit so that if no compost is on the conveyor
belt (i.e., the electrodes pivot down to the surface of the conveyor
belt) the pump will not operate.
The specific components of this system were itemized in
a previous report^ * .
4. Temperature Sensor Probes
As described in a previous section of this report, the
composting process is a batch-type operation with each batch consisting
of the refuse from one day's operation. This batch is placed in the
digester tank where it remains for a six-day digestion period. During
the digestion period, except for the short period when the compost is
agitated, the temperature of each batch is continuously monitored
with a copper-constative thermo-couple sensing probe, 24 inches long,
which is inserted horizontally into the pile through an opening in
the digester wall. The probe is withdrawn when the batch is agitated
and then reinserted immediately upon completion of this operation.
There are four such temperature probes in each digester,
located at the quarter points of the digester length. The temperatures
-104-
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recorded at the eight locations are recorded on an eight point
strip chart recorder.
The specific components of this system were itemized
in a previous report^ '.
5. Mobile Instrument Console
A new and unique instrument package has been developed
for the Authority by Honeywell, Inc. This is a Mobile Instrument
Console package, shown in Figure 2 , which can travel the length of
the west digester tank. It is equipped with one probe to measure
temperature at five levels, and dew point, CL, and CO,, at four levels
in the compost pile. A second probe measures temperature only at
five levels. A third component of the package is a pressure-sensing
unit which can be connected by means of snap-lock fittings to any
of sixteen pitot tubes permanently placed in every other air duct
which supplies air to the west digester tank. There is also a tap
next to each pitot tube for sampling for dew point and the CL and
CO- content of the air being supplied to the digester tanks.
The two probes are constructed of 3/4" diameter fiberglas
tubing and have a pointed wooden plug in the bottom end. Both probes
have thermo-couples at 6", 24", 48", 72", and 90" from the tip of the
probe, and one has gas sampling ports 32", 48", 64", and 90" from
the tip.
-105-
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FIGURE 2
20 Point Strip
Recorder A --,
20 Point Strip
r~ Recorder B
Mobile Instrument
Console
1. Compost Temperature
Recorder A
2. C>2 Analyzer
3. Compost Data Recorder B
4. Suction Pump On-Off
Switch with Indicator
Light.
5. C02 Analyzer
6. Inlet Air Volume
7. Signal Transducer for
converting signal from
C>2 analyzer.
8. Signal Transducer for
converting signal from
dew probe and ambient
temperature thermo-
couples .
9. Signal Transducer for
converting signal from
C02 analyzer.
FIGURE 2
Mobile Instrument Console
Digester Sensing Probes
-106-
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The instrumentation is arranged so that both probes can
be used to measure temperature concurrently. The temperature from
each of the ten points is recorded on a 20-point strip chart recorder,
Recorder A.
The other variables, i.e., inlet air volume, CCL, CL,
dew point temperature, and also ambient temperature, are measured
and recorded on a second 20-point strip chart recorder, Recorder B,
which has a stepping switch to operate solenoid values to route the
sampled gas stream to the appropriate analytical instrument.
The specific components of the unit are listed in a pre-
vious report *• * . The cost of this instrument was $15,600 of which
about $12,400 was for hardware and the remainder for developmental
work and fabrication.
C. METHODS OF SAMPLING
Sampling of raw ground refuse and compost, particularly for
chemical analyses, was done by compositing grab samples early in the
technical evaluation program. This led to erratic and misleading
results. For example, frequently the content of carbon was higher
in raw refuse than in compost. This is contrary to fact inasmuch as
carbon is expected to diminish as a result of composting. The
erratic results were therefore attributable to sampling errors,
which in turn are attributable to the extreme heterogeneity of urban
solid waste.
-107-
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An alternative sampling procedure was developed which circum-
3
vented the problems of grab sampling. Approximately 0.5 yd of waste
was mixed as much as possible without reducing particle sizes. This
usually was done with a shovel or fork. One half of the waste was
prepared for analysis. A portion of the remainder was placed in a
bag made of 16 mesh Fiberglas screen. The bag had an approximate
capacity of 1.5 ft . The bag was then placed in the digester within
the production quantity of waste for treatment. Treatment time and
conditions were varied according to need. The bag of waste was re-
trieved from the digester and prepared for analysis.
Samples before and after treatment were prepared for analysis
by passing the waste twice through a riffle sampler (also known as
a Jones sampler or sample splitter), having a top opening of 8 x 10 in.
and a chute width of 0.75 in. The sample thus reduced in size was
dried to constant weight in an 75°C oven. The sample was ground in
a Mikro-Samplmill to pass a screen having holes of 0.125 in. in diameter.
Subsequently, the sample was reground to pass a screen having rectan-
gular slots of 0.02 in. in width.
D. METHODS FOR CHEMICAL ANALYSES
It was anticipated that chemical analyses would be performed
according to the official A.O.A.C. procedures which are commonly used
in the inorganic fertilizer industry. Subsequently, the A.P.^.A.
procedures^ •> were used as a basis from which modifications might
-108- '-''
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be made. It was recognized from the onset of this demonstration
that many methods of analyses might have to be modified because
of the pioneering nature of this work. The following chemical
analyses were performed on compost and refuse during this demon-
stration: total carbon, total Kjeldahl nitrogen, phosphorus,
potassium, moisture, pH, volatile solids, chemical oxygen demand,
and biochemical oxygen demand.
1. Carbon
The induction furnace combustion method which is a
semi-micro method was used for the estimation of total carbon. The
f7")
APWA method^ } was used with the following modifications:
a) add a small amount of Alundum to the platinum
crucible before putting in samples,
b) use 1.0 N NaOH in place of 0.4N NaOH absorbing
solution,
c) digest precipitate in water bath at 70°C instead
of at room temperature,
d) use medium porosity glass frit filtering crucibles
in place of Gooch crucibles, and
e) dry precipitate overnight at 75°C instead of 1 hour
at 105°C.
Toward the end of the demonstration, Ascarite in small absorption
tubes was used to collect CCL in place of the more cumbersome Ba CO,
-109-
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precipitation method. The results were the same, but the procedure
was simplified. Analytical precision depends greatly on particle
size of the sample. Precision ranged 40 +_ 4.7 percent when satrapies
were ground dry in a Waring Blender. Precision ranged 40 +_ 1.0
percent when samples were ground on the Mikro-Samplmill to pass a
screen having holes of 0.125 in. in diameter.
2. Nitrogen
The K j eldahl-Wi If art h-Gunning method was used for the
determination of organic and ammoniacal nitrogen. A chromium reduction
procedure for the determination of total nitrogen including nitrate
nitrogen was evaluated. The effect was an insignificant increase in
nitrogen and therefore the chromium reduction procedure was abandoned
T71
in favor of the APWA procedure^ '. Typical analytical precision of
the analysis of ground compost was 0.38 +_ 0.025 percent; of ground raw
refuse - 0.48 +_ 0.030.
3. Phosphorus
C7~)
The APWA method^ J failed to give good results because
25 ml of concentrated sulfuric acid did not completely digest the
organic matter and color development was not reproducable. Addition
of mercuric oxide to the acid helped to digest the organic matter,
but this resulted in a precipitate which interfered with the color-
metric determination.
Complete digestion of organic matter was accomplished
by the following combinations of reagents: 1) 25 ml concentrated
-no-
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FLSCL, 15 ml concentrated HNO- as required, and 2) 25 ml concentrated
H2SO. and 2.0 g ICS-CL. However, when color was developed with molyb-
date and done by the APWA method^- ' , and with S2C12 by the Standard
f 81
Methods procedure^ ' , it was not very reproducable.
T9")
The Quimonciac Method*- J was very satisfactory for the
determination of phosphate (as orthophosphate) in compost and refuse.
In the evaluation of this method the average of 9 determinations was
0.424 +_ 0.019 percent phosphate. Recovery of phosphate added to compost
averaged 94.4 percent.
4. Potassium
The A.O.A.C. sodium tetraphenylboron method1- J was satis-
factory for the determination of potassium. However, it was used on
a very limited basis.
5. Moisture
Moisture in refuse and compost was determined by both
f 71
the oven drying and the infrared methods v } , Samples which were to
be analyzed were never subjected to temperatures exceeding 75°C.
Data reported on a dry weight basis was calculated from moisture
values obtained from oven drying subsamples to constant weight at
103°C.
An Ohaus moisture balance was used for the determination
of moisture in samples taken for process control purposes. Moisture
values obtained with this instrument were quite reproducible (+ 0.5%),
-------
and they correlated well with the oven drying procedure. The heat
intensity at which samples were dried was not critical. Drying was
rapid and the sample weights equilibrated after 20 minutes when the
power ranged from 105 - 140 watts. Distance between sample and
the lamp was set at 2 in. At 80 watts, drying time was prolonged
to 45 inin. At 160 watts, drying time was reduced to 15 min., but
the sample was scorched.
6. Hydrogen-Ion Concentration
Hydrogen-ion concentrations (pH) were determined by
f 71
the APWA method^ J . However, it is not necessary to use CCL-free
L*
distilled water for suspending the samples if the water is used
within 2 weeks after it has been distilled. Furthermore, most raw
refuse and compost has considerable buffering capacity.
For routine work, particularly in the field, pH indicator
paper was usefull and convenient.
7. Volatile Solids
Volatile solids in samples of raw refuse and compost were
f 71
determined by the APWA method1- J . Although there was no reason to
suspect the method for its determination, volatile solids data were
never reliable as an indicator of loss of putrescible matter as a
result of composting. It would appear that volatile solids data are
an excellent indicator of putrescible matter loss because they approx-
imate organic matter loss. Apparently, the lack of reliability is
attributable to sampling errors.
-------
8. Chemical Oxygen Demand
The determination of chemical oxygen demand (COD) of
solid materials is similar to that of liquids except that a wetting
agent is required to obtain a reasonably uniform suspension of solids
for subsequent sampling and dilution. The most accurate and precise
results were obtained when using Aquarex (DuPont) as a wetting agent.
A 1.8% solution of this material is made up with distilled water. A
0.5 g sample of ground compost or refuse is weighed and placed in an
800 ml beaker with a magnetic stirring bar. Samples were ground with
a Mikro-Samplmill to pass a screen having holes of 0.125 in. in di-
ameter. One-hundred ml of the 1.8% solution of Aquarex is added and
the mixture is stirred until all the compost is wetted. This usually
occurs in a minute or so. Four-hundred ml of distilled water is added
and stirring is continued. A 10 ml Mohr pipet with the tip enlarged
is used to take the sample. The sample (10.0 ml) is placed into the
COD flask. From this point the procedure is the same as that found
(81
in Standard Methods^- }. An equivalent quantity of Aquarex is used
in the blank. Therefore, subtraction of the COD value of the blank
from the COD value of the sample containing the wetting agent gives
the COD value of the sample only.
9. Biochemical Oxygen Demand
The determination of biochemical oxygen demand (BOD)
of solid materials is similar to that of liquids. Just as in the
-HIS-
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COD determination the solids must be uniformly suspended in a
liquid for accurate sampling and dilution.
The oven dried waste (5.00 g) is homogenized for 5
minutes with 495 ml of BOD dilution water in a Waring blender. Raw
ground refuse and compost can be satisfactorily homogenized with the
Waring blender. It is not necessary to fine grind these materials.
This suspension was subsequently diluted in the same manner as a
liquid waste. Following this preparation, the analytical procedure
is similar to that described in Standard Methods'- '. Fresh sewage
is used as seed after storage for 24 - 48 hrs. at 20°C. It is
filtered through Whatman No. 12 paper to remove suspended solids.
The seed is used at a concentration of 10 ml per liter of suspended
waste. This high concentration is always desirable, but it is neces-
sary if the waste had been dried previous to analysis.
The BOD test has many disadvantages even when used for
the analysis of liquids. The disadvantages are compounded when the
test is used for the analysis of solids. Among these are the long
incubation period, poor precision, and the fairly involved procedure.
However, the BOD test proved to be the most reliable one for determin-
ing the reduction of putrescible matter resulting from composting.
The advantage of the BOD test is that it is a measure of
the carbon which is available for biological utilization. This is
important because composting is a biological degradation process.
-------
Consequently, the BOD value of raw solid waste is an approximation
of the carbon compounds which could be decomposed by composting.
Also, the BOD value of composted waste is an approximation of
further decomposition potential. It is this decomposition potential
which leads to odors when an immature compost becomes wet. The
difference between BOD values before and after composting is a
measure of what was accomplished during composting.
It appears, therefore, that there is a need for a
method for the determination of carbonaceous matter which can be
utilized by biological systems.
10. Putrescible Matter
One of the more vexing problems facing researchers in
solid waste treatment is the lack of methods for the determination
of biological stability of waste, i.e., its content of putrescible
matter. Process evaluation at the Gainesville plant and elsewhere
depends on knowing the stability of the waste at various stages of
treatment. With this knowledge it is possible to determine the
efficiencies of various processes.
Waste stability at Gainesville was determined by the
following tests: the standard BOD, the Hach modification of the
BOD, the COD, volatile solids, and C/N ratio. None of these tests
was fully satisfactory for various reasons. This shortcoming usually
necessitated making all analyses on each sample.
-115-
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A reportedly rapid, simple, and inexpensive method for
the determination of the completion of composting was evaluated for
fl21
applicability. Jamr ' and others developed the method to determine
the quantity of the putrescible matter in organic wastes. Their
method is based on the principle that volatile acids are produced
during the anaerobic decomposition of fresh raw organic matter. On
the other hand, stable organic matter subjected to anaerobic conditions
fails to yield appreciable quantities of acid because the acid pre-
cursors have been decomposed previously. Generally, their method
involves the incubation of a 5.0 g sample in a screw cap tube at 55°C.
The pH of the sample is determined at 0, 24, 48, and 72 hours. The
completion of composting is indicated by a pH of 7.5 after the sample
has been held anaerobically for 24 hours at 55°C. The 48 and 72 hour
analyses are confirmatory.
The test was modified somewhat for application to solid
wastes which were encountered at the Gainesville plant. Samples were
ground on a Mikro-Samplmill to pass a 0.2 mm screen. About 2.5 g of
sample were placed in 20 x 100 mm screw top test tubes. Water was
added to the tubes to saturate and cover the waste. No further modifi-
cations were necessary. The pH of the samples were determined by a
meter.
It can be expected that samples representing wasteJP which
had received increasing treatment, would under test produce progressively
less acid. This was not always borne out. Sampling errors were shown
-116-
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to be the cause of this inconsistency. A sample of stable compost was
tested by this method to give base line data. Putrescible matter at
two concentrations was added to the stable compost and the mixture was
tested again. The pH which developed on incubation of the samples
containing the high concentration of putrescible matter was lower than
that of samples containing the low concentration of putrescible matter.
Also, the pH which developed on incubation of the latter samples was
lower than that developed in unamended samples.
Details on the development of this test for the deter-
mination of putrescible matter in compost and refuse were reported
previously^ '. The work showed that putrescible matter in solid waste
can be roughly quantitated on the basis of acid production following
anaerobic incubation. Results were available at 24 hours, but verifi-
cation at 120 hours was desirable. This method was developed late in
the Gainesville Demonstration Project and, therefore, was not used
routinely in the evaluation of the Metro process. It appears to have
the greatest potential of any evaluated on this Project. Further develop-
ment leading to minor modifications is necessary. What is more important,
however, is the repetitive use of the method to establish its reliability.
Variation in subsamples has a profound adverse effect which
might be offset by use of much more subsample. Determination of pH by
the use of a meter was cumbersome and much more precise than necessary.
The use of pH indicator paper would be more convenient and would result
-117-
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in less sample disturbance. The use of inoculum (seed) would probably
increase the precision of the test because it is expected that the
indigenous microbial population will vary widely among sample types.
E. BACTERIOLOGICAL ANALYSES
Certain bacteriological analyses were made of raw refuse and
compost to determine the public health quality of these materials.
Selection of these analyses was arbitrary because standard methods
for the bacteriological examination of refuse and compost do not exist.
Examination for coliform bacteria was accomplished because it is common
practice in wastewater technology. Examination for members of the
Salmonella genus of bacteria was also accomplished because of the poten-
tial this group has for being carried through the food chain of man and
animals.
1. Coliform Bacteria
The State of Florida, Department of Health and Rehabili-
tative Services, required the daily examination of refuse and compost
for both total coliforms and fecal coliforms. Samples were taken three
or four times each day and composited to represent the entire day.
Samples of the material were taken after primary grinding and after
final grinding. After considerable experience, the sampling frequency
was reduced to twice weekly and the analysis for total coliforms was
dropped.
Membrane filtration techniques for the detection of
coliforms were unsuccessful because of the particulate nature of the
-118-
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f QA
sample. The multiple tube fermentation technique1- } was satisfactory
and was used for this purpose on the Gainesville project for about
two years. Log dilutions were made from 5.0 g of moist solid material.
Transfers of each dilution were made to lactose broth and the con-
firmed test was carried out. Details of the procedure were previ-
(2 31
ously described ^ ' J . Numbers of coliforms, the coliform index,
was expressed as the most probable number (MPN) on a dry weight basis.
2. Salmonella
The State of Florida, Department of Health and Rehabila-
tative Services, also required the daily analysis of raw refuse and
compost for Salmonella. The same samples taken for coliform analysis
were used for Salmonella analysis. Figure 3 shows the screening pro-
cedure .
3" Extrinsic Bacteria
The analyses of raw refuse and compost for coliforms and
Salmonella did not satisfactorily indicate the public health quality
of these materials. An. alternative procedure was developed.
Raw ground refuse was inoculated with a known number of
various pathogenic and heat tolerant bacteria. The ground refuse
was placed in a Fiberglas bag and treated in the digester. After
treatment, a quantity of the same refuse was recovered and assayed
a second time. The numbers of organisms before and after treatment
were compared to determine the effect of composting on the growth
-119-
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FIGURE 3 FLOW SHEET FOR SALWELLA SCREENING
Each 10 gram Subsample
\
Plant j.nto-25 ml. teirathionale broth
| Incubate 48 hours at 37°C
Streak two_ plates of brilliant green agar
i Incubate 24 hours at 37°C
Pink or colorless colonies -
pick to TSI slants
(stab and streak)
I Incubate 24 hours at 37°C
Green colonies -
discard as lactose fermenters
Butt acid yellow
Slant alkaline (red)
and/or H2S+ blackening) -
Inoculate 1, Urea agar
2. Phenylalanine agar
3. Simmons citrate agar
Incubate 24 hours at 37°C
Butt and slant acid (yellow)-
Discard as lactose fermenter
Urea - (no red color)
Citrate + blue
Phenylalanine - no green
color with test reagent
Possible Salmqnellaj,
re-inoculate TSI slant
Urea+ (red)
Citrate + (blue)
Phenylalanine
(blue to green
pigment and grape like
odor)
discard as
Pseudomonas
eruginosa
Incubate 8-24 hours at 37°C
Proceed with testing for Somatic,
"0" antigens with polyvalent "0"
antiserum
^
Citrate +_
Phenylalanine
+ (green with test
reagent) -
discard as
Providence
Urea+ (red)
Citrate + (blue)
Phenylalanine +_
discard as Protei
Poly "0" positive
(good agglutination)
Test witn "0" groups Al
Poly "0" negative
(no agglutination)
discard as non Salmonella
Probably Salmonella
"0" i,roup positive -
Probably Salmonella
All groups negative-
Test for washing "Vi"
antiserum
"Vi" positive
Boil for 10 minutes to
destroy "Vi" antigen
then test again for
;'Q" Croups A-l
Negative -
discard as non-Salmonella
after re-testing biochemical
and serolo^ic properties
Vi negative
Discard after
repeating biochemical
and serological tests
as non-Salmonella
-------
or survival of these extrinsic microorganisms. This procedure
yielded information on the effects of composting on a specific,
known microorganism.
The test organisms were Escherichia coli, three species
of Salmonella, and Bacillus stearothermophilus (American Type
Culture Collection No. 7953). E. coli is likely to indicate con-
tamination of intestinal origin, the Salmonellae are important patho-
gens in the food chain of man and animals, and B_. stearothermophilus
is an extremely heat tolerant species of bacteria. All organisms
were grown on Bacto-Tryptone Glucose Extract Agar (TGEA) to obtain
inocula. The organisms were washed off the surface of the agar with
a sterile buffer consisting of 1.0 g each of KH-PO. and KJffO. in
1000 ml of distilled water. This suspension was diluted 1 part by
volume into 100 parts of tap water and then mixed thoroughly with
about one cubic foot of raw ground refuse. Inoculated refuse (10.0 g)
was assayed to determine the initial number of test organisms. The
remaining refuse was placed in Fiberglas bags for treatment in the
digesters. The 10 g sample was added to 500 ml of buffer and was
homogenized in a Waring blender for 5 minutes. Dilutions of this
suspension were made and plated on either TGEA to count E_. coli and
B_. s tearo thermophilus or on Bacto Brilliant Green Agar to count
Salmonellae. Plate counts were made in the same manner from compost
which had been digested in the Fiberglas bag.
-------
There were several variations made to obtain additional
information on the growth of these extrinsic microorganisms in a
composting environment. In addition to composting the seeded refuse,
refuse inoculated with these pure cultures of Salmonellae, was incu-
bated in the laboratory to determine the effect of lower temperatures
on the survival of these organisms in the presence of ground refuse.
This was done by incubating approximately 50 g of seeded refuse at
99°F (37°C) in a loosely covered beaker. Counts of Salmonellae were
made before and after incubation.
In another variation, refuse seeded with S_. paratyphi
was subdivided into two quantities. One quantity was placed along
the wall of a digester where temperatures are known to be lower than
most of the contents of the digester. The temperature rose from 93°F
to only 108°F in one day. Another quantity of refuse was placed deep
in the digester where temperatures are known to go higher. The temp-
erature of this refuse rose from 93°F to 131°F in one day. After a
sample of the first quantity was taken for a plate count determination,
it was returned to its original location in the digester for further
treatment. The temperature rose from 108°F to 126°F in three additional
days.
In a third variation, both spores and vegetative cells of
B. stearothermophilus were used to seed refuse. The spores of any
organism are expected to be considerably more heat resistant than cells
-122-'
-------
of the same organism. To obtain spores, a culture was allowed to
age on TGEA. The spores were washed off the agar with buffer. The
buffer suspension was pasteurized at 176°F for 10 minutes to kill
any vegetative cells which might not have sporulated. The refuse
was then seeded and assayed by plate count in the usual manner.
In a fourth variation, a culture tube containing a
buffer suspension of vegetative cells of B_. stearothermophilus was
placed in the digester for one day. A plate count was made before
and after treatment in the usual manner.
During this work, refuse was being moistened with raw
sewage sludge except during the one experiment in which refuse seeded
with E. coli was composted for 12 days. Bags containing seeded refuse
were placed in the digesters at levels ranging from 65 - 80 inches
above the bottom. The probable temperature ranges at this level at
varying days of treatment are as follows: day 1 - 110-130°F, day 2 -
130-140°F, day 3 - 140-160°F, and day 4 and beyond - 140-160°F.
F. OCCUPATIONAL HEALTH STUDIES
Bacteriological analyses described in the previous section
were made principally to show the public health quality of the pro-
duct. That work delineated potential hazards to the compost consuner.
Studies described in this section were made primarily to define
potential and actual hazards to plant personnel.
-------
1. Physical Examinations
All plant personnel and most administrative personnel
were given periodic physical examinations to detect clinical mani-
festations resulting from their activities associated with the compost-
ing of urban solid waste. It was planned to give the first examina-
tion prior to any exposure to solid waste, and then periodically at
intervals of 6 months. In addition to general physical examinations,
the health of workers was further judged on the basis of the following:
Mantoux and histoplasmin skin tests, hematocrit, hemoglobin, and VDRL
blood tests, chest x-rays, stool examinations for intestinal parasites,
and urinalyses for hydrogen ion concentration (pH), specific gravity,
glucose, acetone, protein, and occult blood.
Dr. George Little, a local physician, conducted these
clinical studies.
2. Noise levels
Various operations in the processing of refuse fox compost-
ing generate considerable noise. Two noise level surveys were conducted
to determine whether noise was a hazard. A type 1555-A Sound-Survey
Meter (General Radio Company) was used for this purpose.
3. Intestinal parasites
The presence of viable parasites in compost would indicate
insufficient treatment. Accordingly, samples of raw refuse and compost
were examined for parasites.
-------
Samples were collected and sent to the Division of
Research and Development, Bureau of Solid Waste Management, Cincin-
nati, Ohio, for analysis. No attempt was made to cool or otherwise
preserve the samples during shipment. Samples were prepared for
examination by shaking in the presence of physiological saline and
glass beads. Next, samples were either examined directly with a low
power microscope or prepared further and then examined microscopically.
When further preparation was required, the biological material was
separated from debris by either the brine gravity flotation method
or Willis*- * or the formalin ether sedimentation method of Ritchie^ '.
4. Airborne Particulate Matter
The air in dusty areas of the plant was sampled for parti-
culate matter as an indicator of potential public health hazards. Parti-
culate matter was determined with both a standard high volume sampler
for total particulates and with an Andersen sampler to determine particle
size distribution.
An 8 x 10" Fiberglas filter was used in the high volume
sampler which is 99% effective in removing particles ranging in size
from 0.1 micron and larger. The flow rate of air thorugh the instru-
ment was 39.2 cubic ft. per minute. The instrument was placed in
the plant midway between the primary and secondary grinders. It was
operated for 4 3/4 hrs. in the afternoon of October 17, 1969.
-125-
-------
Particle size distribution (weights of particles of
various sizes) was determined at two locations in the plant with the
Andersen sampler. Two determinations were made on September 8, 1969,
at the operators platform. Duration of sampling was one hour in the
morning and 30 minutes in the afternoon. One determination was made
on September 9, 1969, at the picking table. The duration of that
sampling was one hour. Particle weights were determined by weighing
the glass collection plates before and after exposure and by sub-
tracting the two values.
G. ARTHROPOD AND RODENT CONTROL METHODS
The control of arthropod and rodent pests depended both
on the application of existing technology and on the development
of new technology. Rats and roaches were readily controlled by
poison baits and commonly used sprays. The control of flies depended
on a study of their habits as they relate to solid waste and compost.
A successful fly control procedure was developed on the basis of this
study.
The procedures for the control of rodents and roaches were
presented as follows:
Rodents: The sealing of the holes around the water pipes
entering the washroom reduced the rodent population, so that rats and
mice are rarely observed in the Compost Plant. Liquid and dry Pival
baits are available for the control of rats, but good housekeeping is
the best control.
-124*
-------
Roaches: Roaches are normally controlled with a monthly
application of a 2.0$ Diazinon spray. The best control of roaches is
by good housekeeping.
These procedures should interest not only operators of compost
plants, but also operators and administrators of solid waste manage-
ment programs.
The procedures developed for the control of flies are pre-
sented in another section of this report. The methods and results
are presented together for purposes of continuity.
H. RESULTS
1. Composition of Refuse as Received At the Plant
Knowledge of the composition of refuse received at
a compost plant is important to both plant design and operation.
It is particularly important in planning salvage operations.
Refuse was analyzed for major constitutents to deter-
mine variation within given days, variation between given days, and
variation between seasons. Analyses were made on January 24, July 8,
August 21, and every day during the period of September 10 - 16, 1970.
Samples for the first two dates were taken at the plant receiving
platform. The final sample of about 2 yd was comprised of four sub-
samples taken throughout the day to represent the composition of refuse
for those two days. Samples for the remaining analyses were taken at
the oscillating conveyor ahead of the picking platform. Refuse located
-------
at the last two feet of the output end of the conveyor was collected
at designated times throughout the day. This procedure led to un-
biased sampling. Samples were sorted by hand into ten major constit-
uents with further subdivision of paper and metal because of their
salvage potential. All constituents were weighed and reported as
percentages of the total refuse sample on a wet weight basis.
Table 2 shows the composition of refuse received at the
plant on August 21, 1969, at designated sample times and the day's
average. The data show typical variations throughout the day. Table 2
shows the daily averaged composition of refuse during the period
September 10 - 16, 1969, and the weekly average. Table 2 shows a
summary of refuse composition on the designated sampling dates. The
data collected in September show that there is great variation of
each refuse constituent throughout each day. Detailed results represent-
ing designated times of each of the 6 sampling days were presented in
a previous report^ * . There was less variation noted between days.
Paper constitutes about 50 percent of the total refuse. Salvageable
paper constitutes about 23 percent of the total refuse. Food waste
(garbage) averaged about 5 percent, and garden wastes averaged about
13 percent. Ferrous metals comprised 7.5 percent and non-ferrous metals
(mainly aluminum) comprised 0.6 percent of the refuse. All data result-
ing from this study represent waste generation by the local community
inasmuch as all the waste, with one exception, is delivered to the
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compost plant. Demolition wastes and bulky wastes such as automobiles
and appliances were taken directly to landfills.
2. Composition of Material Removed by Magnets
The composition of the material removed by the magnets
is important information for use in metal salvage considerations. At
the time of this study (March 19, 1969) two electromagnets were used
to remove ferrous metals. They are located after the secondary grinder
and after the digesters. A sample of the material removed by each
magnet was collected, weighed, dried, and burned to determine its compo-
sition. The compost leaving the final grinder was also examined for
the amount of metal present.
The samples taken at the magnets were burned with an
acetylene torch to approximate conditions in a metal salvage incin-
erator and to simulate metal which would meet market requirements.
The metal and ash were then separated and weighed. The following
compilation shows the composition of material removed by the magnets:
Composition of Material, % of Total
Magnet
Location
After Secondary
Grinder
After Digesters
Moisture Volatile
Solids
3.8 6.6
8.7 13.5
Metal Ash
88.5 1.1
68.2 9.6
Sample
Size
Ibs.
10.4
3.9
-------
A sample of final grind compost (5.7 Ibs.) was spread
out on a flat surface and a hand magnet was used to take out the small
amount of remaining ferrous metal. The nonferrous metal (mainly alum-
inum) was removed by hand picking. The sample contained 43.8 percent
moisture, 0.14 percent ferrous metal, and 0.06 percent nonferrous
metal. These results show that the two magnets are removing almost
all of the ferrous metal and that the first magnet is removing cleaner
metal than the second one. The indication is that the material taken
at the magnet after the secondary grinder has definite salvage poten-
tial if a profitable market can be found.
3. Bulk Density of Refuse as Placed in Digester
The bulk density of the refuse as placed in the digester
is a valuable parameter which may be used to determine digester size
and to design refuse handling equipment.
Records were kept for a three day period beginning Monday,
March 10, 1969, of the refuse received, paper salvaged, non-compostables
removed at the sorting platform, material removed by the magnet after
the secondary grinder, and compostables to the digester:
ITEM TONS PERCENT
Refuse Received 481.5 100.00
Paper Salvaged 40.5 8.41
Non-Compostables Removed 102.5 21.29
At Sorting Platform (74.9) (15.56)
At Magnets (27.6) (5.73)
Compostables to Digester 338.5 70.30
-------
At the time the records were made, water was being added
at the secondary grinder in lieu of sewage sludge. The amount of
water added was 740 gallons per hour. The estimated total amount of
water added was based on the number of hours (19.4) that the conveyor
belt operated. The amount of water added over the three day period
was, therefore, estimated to be 59.8 tons.
The dimensions of ground refuse mass in the digester
were 20.0 ft. x 7.16 ft. x 223.5 ft. The volume was 1185 cubic yards.
The moisture content of a grab sample of the material as
placed was 44 percent. The total weight of the refuse plus water
placed in the digester was 398.3 tons.
The resulting bulk density is only an approximation because
of the difficulty of determining the amount of water added and of measur-
ing the height of the uneven surface of the refuse in the digester. An
error analysis made of the data indicates that the bulk density value
is 670+60 lb/yd3.
4. Moisture Content of Refuse and Compost
Refuse moisture contents were determined periodically at
several points in the composting process. This information is useful
for process control and for research.
Moisture analyses were made on samples of refuse just
before secondary grinding on refuse as placed in the digesters, and
on compost immediately after final grinding. Data derived from the
-------
first sampling point gave an excellent estimate of the moisture
content of refuse as it is received at the plant and hence the
amount of sewage sludge that may be added. Some error was intro-
duced by the prior separation of the noncompostable fraction.
However, grinding prior to sampling greatly enhances the homo-
geneity of the sample resulting in reasonably precise determina-
tions.
Samples of about 500 g were collected for the deter-
mination of moisture in refuse just prior to secondary grinding.
Samples of refuse and compost from other sources usually ranged
from 10 - 100 g. All samples were dried at 75°C to constant weight.
Normally two days were required to dry the sample. Table 3 shows
a summary of all moisture analyses made during the period of May -
October, 1969. Moisture contents are reported as a percent of the
wet weight. The increased moisture of the refuse as placed in the
digester in October is a result of more sewage sludge addition.
Data for three months has been gathered on moisture
content of the refuse samples taken before the secondary grinder.
Rainfall data were compared with refuse moisture data. The follow-
ing compilation shows the effect of rainfall on the moisture content
and weight of refuse received at the plant.
Total % Moisture Tons Tons Rainfall
Month - 1969 Tons Liquid Solid Inches
August
September
October
3376
4068
3718
28.5
30.0
23.6
960
1219
877
2416
2849
2841
9.5
11.5
1.2
-135-
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The refuse total for October was adjusted upward 220 tons
from the actual 3498 tons received to correct for two days when the
plant was not operated. August was a low tonnage month mainly because
of the decrease in enrollment at the University. In September, the
students returned and a record 4068 tons of refuse was processed.
Even after adjusting the tonnage for October, the refuse for the
month is about 350 tons less than that for September. The data show
that the difference between the total refuse weights for September
and October is mainly due to the difference in moisture contents.
The refuse solids calculated for the two months agreed quite closely.
These data indicate that the actual refuse production for the months
of September and October was about the same, even though the wet
weights alone showed considerable difference. This difference was
attributable to rainfall. A plot (not shown) of percent moisture of
the compostable refuse versus inches of rain per month for the three
months of data collected to date indicates a linear relationship.
•>• Sewage Sludge Utilization
The treatment of raw sewage sludge in conjunction with
composting refuse would be highly desirable in that it has the poten-
tial for greatly reducing waste treatment costs.
The digestion of sewage sludge by conventional processes
is costly and inefficient. The cost of sludge treatment and disposal
by conventional means approaches 50 percent of the total cost of
treating sewage. By contrast, the treatment of sewage sludge by
-137"
-------
composting along with refuse adds very little to the cost of refuse
composting. Also, sludge contains nutrients, particularly nitrogen,
which might enhance waste decomposition and enrich the compost pro-
duct. Finally, the refuse as it is received contains insufficient
moisture for good decomposition to occur. This necessitates adding
water. Sludge might replace water for this purpose.
Digested sewage sludge has been treated intermittently
at the Gainesville compost plant during the period from June, 1968
to April, 1969. During that time the feasibility of composting
refuse-sludge mixtures was shown. The sludge supplement did not
hinder refuse decomposition. It did reduce the numbers of coliform
bacteria which survived the composting process. Tests over an ex-
tended period of time indicated that the probability of survival of
pathogens during digestion was very low.
Up to April, 1969, sludge had merely been disposed of
by composting after it had been digested at the sewage treatment
plant. The feasibility and safety of treating raw sewage sludge had
been indicated. Therefore, the treatment of raw sludge by composting
was initiated on April 28, 1969. Ideally, the compost plant should
have the capability for treating the entire quantity of sludge and
solid waste produced by a given population. This would eliminate
the need for sludge digestion at the sewage treatment plant. The
resulting savings would offset operating costs at the compost plant.
-138-
-------
Several problems had to be solved before this goal could be achieved.
The first raw sludge to be treated had a solids content of only 1.5%
(98.5% water). It would not be possible to consume all of the city's
sludge if its moisture content were that high. The result would be
a refuse-sludge blend far too wet to handle and compost by the exist-
ing process. Table 4 shows that because of this restriction only 10%
of the total raw sludge produced by the City was treated by composting
during May, 1969.
Another obstacle to sludge addition was the narrow con-
veyor belt on the tripper which is used to place ground refuse in the
digester. The conveyor would bind occasionally and stop when the refuse
moisture content approached 60%. This stopped the entire operation
of the plant. A wider conveyor belt was installed on October 4, 1969,
and there has been no more difficulty.
A two inch plastic pipe was installed to carry sludge
from the sludge reservoir to the mixing screws. This proved to be
undependable because of periodic breaks in the pipe and separation
of the pipe joints. To insure a more dependable system, the plastic
pipe was replaced by a four inch galvanized steel pipe on October 20.
The City installed a six inch cast iron pipeline to
carry sludge from the sewage treatment plant to the sludge reservoir
at the compost plant. The new pipeline was first used on October 21,
and it has worked well since then. Prior to that date, sludge was
trucked to the compost plant.
139
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A Moyno pump is used to transfer sludge from the sludge
reservoir to the mixing screws. It is the only means for controlling
the ratio of sludge to refuse. It is not practical to adjust the
flow of refuse for this purpose. Although the pump speed can be
varied, a convenient means to keep the sludge-refuse ratio constant
by varying the pump speed has not been developed. Consequently, the
only existing means for controlling this ratio is to turn the pump
on or off. This is only marginally satisfactory and a better means
for regulating the sludge-refuse ratio is urgently needed.
Attempts are being made to compost the entire output of
sludge from the City. Starting on October 15, 1969, a thick sludge
having a solids content ranging from 5-8% has been received. The
thick sludge was obtained by withdrawing it from the bottom of the
sewage plant digesters after it had settled. Using sludge of this
thickness, it should be possible to compost the entire sludge output
of the sewage treatment plant. The system existing at the termination
of the project was somewhat expedient. A digester was used to thicken
sludge by settling. During this process the sludge was partially
treated. This was a temporary measure pending the completion of
construction at the sewage treatment plant. Nevertheless, it now
appears feasible to compost the entire sludge output from the City.
The addition to the sewage treatment plant recently has
been completed. The existing plant (trickling filter) has a design
-------
capacity of 5.0 MGD. The addition (contact stabilization) has an
additional design capacity of 4.5 MGD. The addition to the sewage
treatment plant does not include sludge digestion tanks because it
is expected that the compost plant will have the capability for
treating the majority of the sludge produced. A 30 foot diameter
picket type sludge thickener is included in the sewage treatment
plant addition and is expected to dewater sludge to a solids content
of 4 to 6 percent.
Records maintained by sewage treatment plant personnel
show that during the period of May through September, 1969, about
1.33 million gallons of raw sludge were withdrawn each month. The
contributing population was about 52,000. Sludge solids analyses of
raw sludge delivered to the compost plant have averaged about 1.5%.
Based on these figures, the sludge solids production of the City
appears to be about 85 tons per month.
The compost plant is currently used for treating all
the refuse from the City, University, and County near the City. The
contributing population is estimated as follows:
City of Gainesville = 52,000
university of Florida = 11,100
Alachua County (near City) = 6,400
Total 69,500
-142-
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The average amount of refuse received was 3500 tons
per month based on data for July through December. The October
receipts have been adjusted to allow for a few days when plant
modifications were being made and no refuse could be accepted.
An estimate of the degree of sludge thickening required if all of
the City's sludge is to be composted can be made on the basis of
typical amounts of refuse and sewage sludge produced and their
moisture contents. The following theoretical compilation is based
on raw sewage sludge production of 1.33 MG per month at a solids
content of 1.5% and refuse production of 3500 tons per month with
3000 tons per month of compostables at 30% moisture.
SLUDGE SOLIDS REQUIRED TO COMPOST ALL SLUDGE
Sewage Sludge
Sludge Plus Refuse
Ratio of Sludge To
Compostable Refuse
% Solids
4
5
6
7
8
Total Thousand
Gallons Per Month
500
402
334
286
250
% Moisture
57.2
53.5
50.5
48.2
46.2
Gal /Ton
167
134
111
96
84
This estimate shows that 134 gallons of sludge per ton of
compostable refuse would be required to dispose of all the sludge,
provided the solids content is 5%. Thicker sludge would require less
-143-
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volume and may be necessary during period of heavy rainfall. The
two digesters at the sewage treatment plant (0.5 MG each) could
serve as additional sludge storage reservoirs during periods when
the refuse is wet.
An estimate of the sewage sludge treated at the compost
plant during October, 1968, was made based on records of volume and
percent solids:
Amount of Sludge Composted During October, 1969
Period
1st to 14th
15th to 31st
WIOLE MDNTH
Gallons
102,780
138,080
240,860
Tons
435
586
* Solids
1.5
5.8
Tons Solids
6.5
34.0
40.5
The 40.5 tons of sludge solids estimated amounts of 47.5% of the
85 tons produced by the City each month. The calculations show that
most of the sludge was treated during "the last half of the month when
thicker sludge was being treated. An estimated 100,000 gallons of
water was added in place of sewage sludge during October because of
sludge handling modifications. The combined amount of sludge and
water used for the month was, therefore, 340,000 gallons.
Data from the period in October when thick sludge was
added indicate that the compost plant can take all the sludge from
the City. During the week of October 27 through 31, a total of
-------
73,000 gallons of thick sludge (about 6 to 8% solids) was mixed
with 610 tons of compostable refuse resulting in a sludge-refuse
ratio of 120 gallons per ton. These results and the previous
theoretical calculations indicate that a strong potential exists
for consuming all of the sludge produced by the City.
6. Effect of Sewage Sludge on Refuse Decomposition
The incorporation of raw sewage sludge into compost-
ing refuse may be a successful method for sludge disposal. Addi-
tionally, the cost of this type of sludge disposal is expected to
be very low as compared with conventional methods. Thus, the cost
of composting might be credited with the savings realized by this
type of combined treatment.
This important aspect of composting was studied to deter-
mine its technical and economic feasibility. The use of digested
sewage sludge as the moistening agent for ground refuse was initiated
on June 5, 1968. City water had been used prior to that date. When
experience from plant operation showed that sludge could be used and
laboratory studies showed high probability for pathogen destruction
raw sewage sludge was used for moistening. This practice was started
on April 28, 1969. This presented an opportunity to compare the effect
of the two moistening agents on refuse decomposition. This was done
by comparing C/N ratio reduction of water-moistened refuse with that
of sludge-moistened refuse. Table 5 suggests that the reduction of
-]kS-
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-146-
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C/N ratio was approximately 60 percent greater when sludge had been
used as compared with water. However, this difference is not con-
vincingly significant because of the limited analytical precision.
This work was based on the analysis of compost samples which did
not necessarily represent comparable samples of raw refuse. After
the development of the Fiberglas bag technique, the same sample could
be analyzed before and after composting.
Experiments were designed to determine the effect of
sewage sludge on the decomposition of refuse and on the quality of
the compost product. Raw refuse and compost was analyzed for carbon,
nitrogen (hence the C/N ratio), COD, BOD, and volatile solids. A
greater reduction of C/N ratio and the other three parameters mani-
fested by the sludge amended compost than in the unamended compost
was evidence that sludge enchanced the composting process. In each
experiment a quantity (usually 1/4 - 1/2 yd ) of raw ground refuse
was mixed with a shovel or fork. A sample of this refuse was analyzed.
Other samples were put into Fiberglas bags which were placed in the
digester within the production quantity of refuse. Sewage sludge in
varying quantities and concentrations was added to still other samples.
These were analyzed before and after composting.
A total of 8 experiments incorporating 44 treatments and
replications were made to determine the effects of sludge supplements
on refuse composting. Detailed results were reported previously^- '•'.
-------
Analyses for C/N ratio, COD, BOD, and volatile solids were never
consistent in showing that sludge stimulated waste decomposition.
The BOD test was the most reliable for this purpose, whereas the
volatile solids test was least reliable. There were no indications
from these four tests that sludge could not be treated by composting.
Some experiments showed that high concentrations of sludge added
significant quantities of putrescible matter to raw waste. Further-
more, putrescible matter in the sludge supplemented compost was
frequently higher than that in the unsupplemented compost indicating
that sludge burdened the composting process. The highest concentra-
tion of sludge which was evaluated was 0.64 kg sludge (2.3 percent
solids) per kg of refuse, which is equivalent to 154 gallons per ton.
Even at this high concentration of sludge there were no indications
that the process was seriously hindered.
Sewage sludge also has the potential for enriching the
compost product with nutritional elements. Nitrogen is the most
important element which might be added by sludge supplements. The
addition of sludge did, in fact, raise the nitrogen content of the
refuse-sludge mixture. The nitrogen content of unsupplemented refuse
typically ranged rom 0.4 - 0.7 percent; that of supplemented refuse
typically ranged from 0.4 - 1.1 percent. There is evidence that
nitrogen was lost during the composting of sludge supplemented refuse.
It can be expected that nitrogen in the form of ammonium compounds
-------
would be lost during the thermophilic stage of composting. The
mechanism of this loss was not studied, but analysis of refuse
heavily supplemented with sludge before and after composting fre-
quently showed a loss of nitrogen ranging from 0.1 - 0.3 percent.
The addition of sewage sludge to refuse had an unex-
pected effect on the coliform populations in the composting refuse.
Table (Section H-12) shows that when water was used to moisten raw
ground refuse the coliform population in the resulting compost re-
mained stationary or increased relative to the original population.
' ' •<"
By contrast, when sewage sludge was used the coliform population in
the resulting compost decreased except possibly during the sampling
period of October 2, through December 31, 1968. Because the usual
standard deviation of the MPN method for the determination of coliform
indices is no better than one logarithmic unit, even this apparent
increase may not be real. The extent of coliform reduction when
sludge was added can be equated approximately to the amount of sludge
being added. For example, during the June 5 - June 26, 1968, sampling
period when sludge was added at the rate of 77.2 gal per ton of refuse,
the coliform population was reduced by four logarithmic units. During
January and February of 1969, when sludge was added for the most part
at the rate of 27.2 gal per ton of refuse, the coliform population was
reduced by only two logarithmic units. In the conduct of this work
there was no assurance that the same refuse was assayed before and
-------
after composting. Thus, the data can be expected to vary widely.
In spite of this, the large number of assays made strongly suggests
that sludge supplements do indeed reduce coliform numbers.
Compost derived from sludge supplemented refuse was
darker than that derived from water moistened refuse. The difference
in color usually was subtile and, therefore, was not noticeable until
the compost had been finely ground for chemical analysis. Color
observations were made on matched samples prepared for composting in
the Fiberglas bags. Untreated control samples, including those which
received sludge and composted controls which were moistened with water,
were light grey and were indistinguishable on the basis of color. When
sludge was added at varying quantities the darkness of color in the
compost was directly related to the quantity of sludge added to the
refuse. Thus, the dark color resulted from the composting of sludge-
supplemented waste and not from the sludge itself. In the 7 of 8
experiments designed to determine the effects of sludge on composting
by the Fiberglas bag method of sampling, there were color differences
among the samples which were attributable to sludge. Experience in
the practice of composting indicates that as compost becomes pro-
gressively stabilized it becomes darker.
Still another effect of sludge on composting was rioted.
Limited data suggest that there is a greater loss of weight resulting
from the composting of sludge supplemented refuse than from composting
-150*
-------
water moistened refuse. In some early work ground refuse in Fiberglas
bags was weighed before and after composting to determine weight losses.
Weight losses of water moistened and sludge moistened compost were
determined in separate experiments according to how the production refuse
was moistened. Experimental variation was so great that no conclusions
could be drawn. In subsequent work weight losses of closely matched
samples were compared. In the only experiment when this was done, water
moistened refuse lost 2.8 percent weight as a result of digestion for
6 days and windrow curing for 30 days. Sludge moistened refuse which
received identical treatment lost 9.3 percent weight. It must be empha-
sized that although this experiment was carefully controlled, the result-
ing data are only single determinations.
Sludge as contrasted with water as the moistening agent
caused several other pronounced effects on the digestion of refuse as
follows: 1) temperatures rose more rapidly, 2) temperatures were more
uniform throughout the digester, and 3) oxygen consumption was much
greater. These results were presented in detail in a previous report*- ' .
In review, however, when sludge was used for moistening, the temperatures
in the digesters leveled off between the second and fourth days. When
no sludge was used temperatures did not level off until the fifth or
sixth days. Uniformity of temperatures at the various levels in the
digester was demonstrated by the narrow range of 162-172°F on the third
day sludge was added. Typically, temperatures ranged from 140-165°F
-151-
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on the third day when no sludge was added. These ranges do not
include temperatures at the bottom 6 inches of the digester which
typically are approximately 100°F, regardless of whether water or
sludge had been added. The high oxygen demand of sludge was also
manifested. When no sludge was added the typical oxygen residual
in the digesters ranged from 16 - 19 percent. When thick sludge
was added this residual concentration was about 9 percent even
though the aeration period was extended from 16 to 28 minutes per
hour.
In conclusion, there was no experimental evidence to
show that raw sewage sludge could not be used as a moistening agent
for refuse which is to be composted. It will be necessary, however,
to furnish additional air to the digesters when large amounts of
sludge are added. An operational problem which is correctable was
occasionally experienced in the plant. If the plant operator becomes
careless and fails to turn off the sludge pump when no refuse is being
processed sludge is spilled about the plant. This gives rise to
various public health problems such as odors and flies. The same
carelessness when water is being used leads to no serious problems.
It was not conclusively demonstrated that sewage sludge enhanced
or stimulated the decomposition of refuse. However, evidence from
a wide variety of experiments and observations suggests that there
was some stimulation as well as enhanced killing of coliform bacteria.
-152-
-------
It is doubtful whether the stimulation of refuse decomposition can
be substantiated on an industrial scale. Attaining the necessary
controls would be impractical if not impossible. Controlled laboratory
work would be more definite and efficient.
7. Decomposition of Refuse in the Digester
The scope of this investigation was centered around
characterizing refuse decomposition in the digesters. The objective
was to optimize the digestion process. In the operation of the plant
process variables such as detention time and aeration rates were
selected arbitrarily, for the most part. Therefore, it is likely
that they could be improved. The effects of process variables on
digestion were determined on the basis of heat production (measured ,
as temperatures), CCL concentration and oxygen concentration.
A series of two experiments was designed to show the
effect of refuse particle size on the rate of decomposition. A
series of 6 experiments was designed to show the effects of adding
sewage sludge and of aerating the refuse at four different rates.
The first two experiments, one of which sludge was used and the
other without sludge, were somewhat preliminary. The other four
experiments, in which the aeration rates were tested, were more con-
clusive. One of the aeration experiments also provided valuable in-
formation on sludge digestion.
-------
a) Decomposition Rate as A Function of Particle Size: The
rate of decomposition was compared in both coursely and finely ground
refuse. Rates were judged on the basis of temperature development in
the digesters. Finely ground refuse was obtained by shredding it
with both the primary and secondary grinders. Coursely ground refuse
was obtained by shredding it with only the secondary grinder. Water
was used to moisten both types of refuse. Temperatures of the finely
ground refuse rose from 120°F to values ranging from 167°F to 183°F
after six days of digestion. Temperatures of the coursely ground
refuse rose from about 115°F to the range of 153°F to 164°F after six
days. Thus, it was indicated that finely ground refuse decomposed
in the digesters at a considerably higher rate. However, other factors
such as moisture content, refuse composition, and air supply may have
influenced the results. The opportunity to use the combination of
grinders never occurred again, and these important experiments were
not repeated.
b) No Sewage Sludge - July 7: The refuse under test was among
the first to be ground with the new primary grinder. The particle size
of the material as placed in the digester was quite small compared with
that of previous operations. The test area was located at the north end
of the east digester. Water addition resulted in an initial moisture con-
tent of about 501. The refuse was held in the digester for 22 days for the
-------
experiment. A Hays Model 621.31:30 Orsat gas analyzer was used for
the determination of oxygen and carbon dioxide. The Minneapolis-
Honeywell instrument console was unreliable for gas analyses, but it
was satisfactorily used for the determination and recording of tempera-
tures. These instruments were used in this entire series of experiments.
In the normal operation of the digesters, the aeration blowers run inter-
mittently. This causes considerable fluctuation in digester gas content.
To minimize these fluctuations, the gas analyses were made just before
the blower turned on.
Figure 4 shows that the temperature and CO- curves paral-
leled each other fairly well whereas the oxygen curve opposed the other
two. This was as expected because heat and C02 are products of decompo-
sition and oxygen is a reactant. Note that the elevations at which the
temperatures were taken do not necessarily correspond with the elevations
at which gas samples were taken. HoweVer, three of the four curves cor-
respond very closely.
About 4-5 days were required before temperatures leveled
off. From 0-2 days, temperatures at all levels were within 15°F of
each other; after that time they diverged rapidly. In the beginning,
temperatures at the 25 in. and 48 in. levels were higher than that at
the 66 in. level. Between the third and fourth days, the temperatures
at these levels reversed and after then they were invariably cooler
with increasing depth. This indicates the decomposition started sooner
-------
200
180
160
140
4J
M
0)
120
100
20
15
jjio
4J
0)
y 5
u D
01
04
20
a)
o
0)
a 5
v
FIGURE i 4
:EMPERATURE, OXYGEN, AND CARBCMDIOXIDE IN DIGESTER - JULY 7, 1969
66" Above Bottom
Begun July 7, 1969
Initial Refuse Height =7.0 Ft.
No Sewage Sludge Added
23" Above Bottom
35"
47"
66"
66" Above Bottom
10 12 14 16
-156- Time in Days
18
20
22
-------
near the bottom of the digester than at the top. Oxygen consumption
and C0~ production tended to verify this. Subsequently, heat was
transferred upwards.
The digesters are forcibly aerated and it is possible
and desirable to keep the oxygen content at a uniformly high level.
However, somewhere between days 9 and 10 the oxygen content of the gas
in the refuse dropped. This happened when refuse was removed from an
area in the digester adjacent to the test area. This allowed the air
to pass freely through the empty digester rather than through the test
waste. On day 11, the air duct valves were closed where there was no
waste and the oxygen level in the test area began to increase to
previous values. The temperature rose from a maximum of 183°F at
11 days to 196°F at 12.5 days due to the large increase in air supply.
On or about day 19, some more waste was removed near the test area.
This caused temperatures to rise again.
This experiment strongly suggests that it would be desir-
able to agitate the waste during its digestion. Agitation would tend
to equalize temperatures and concentrations of oxygen and carbon dioxide.
A logical first time to agitate may be at four days, or the first time
that temperatures at various levels begin to differ widely. That aera-
tion had a cooling effect was shown by the rapidly rising temperatures
at all levels when the aeration rate was reduced.
Oxygen and C02 concentrations were determined frequently
for a period of two hours to determine their ranges as a function of
157
-------
the aeration cycle. Knowledge of these ranges is useful in interpre-
ting Figures 4-11, which consist of data taken just prior to turning
on the blowers. Determinations were made at the 5.5 ft. level. Gas
concentrations were determined only in the north half of the east
digester. One blower serves both the north and south digester halves.
The aeration period was 18 min/hr. for the north half and 20 min/hr.
for the south half. Figure 5 shows that the oxygen ranged from 31
to 9% by Volume during the aeration cycle. Carbon dioxide levels
ranged from 8% to 13%. The gas composition data show that some air
was going to the north half while the south half was being aerated.
This was evidently the result of one of the air duct valves not: being
completely closed. The additional air to the north half resulted in
less variation in the gas composition than would be expected under
normal conditions. The results also show that the refuse in the di-
gester still has a significant oxygen demand after 21 days.
Figure 6 shows the moisture content at 10, 15, and 22 days
in the digester. Samples were inspected for physical appearance on
the twenty-second day of test. Those taken from the upper third of the
digester appeared more decomposed than any other samples. This is the
region of highest temperature during most of the detention period and
the lowest moisture content near the end of detention. The available
data neither support nor discount a possible correlation between these
two conditions.
158
-------
FIGURE 5
OXYGEN AND CARBON DIOXIDE LEVELS IN DIGESTER - JULY 28, 1969
Readings Hade at 5.5 Ft. Klevation after 21 Days in Digester
o
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O
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O
ft.
20
15
10
Off
20
15 *
10 •
North On
Off
South On
18
38 U2 60 78
Time in Minutes
98 102
120
Off
IS
South On
Ilorth On
Off
South On
33 1*2 60 78
Time in Minutes
98 102
120
60.
50
a
-------
c) Raw sewage sludge - August 1; This is the first
experiment in which raw sewage sludge was used as the moistening
agent. The test area was at the north end of the east digester.
The quantity of sludge added was approximately 50 gallons per ton
of refuse moistened. Sludge solids were estimated to be 1.5 per-
cent. The initial moisture was 43%. Air volumes were determined
by the air flow meter on the instrument console. Air was regulated
by the digester duct valves to approximately 4.5 ft per hour per
ft of refuse.
The moisture in the upper third of the digester at
7 days ranged from 43% to 57%. Figure 7 shows that the temperature
rose at about the same rate as in the previous experiment when no
sludge was added. Maximum temperatures achieved were about the same
prior to the disturbance experienced during the first test. Appar-
ently the quantity of sludge added was too small to produce a notice-
able effect.
The compost was removed from the digester after twelve
(12) days and the moisture content and pH at various elevations
measured:
Elevation in Inches % Moisture pH
87
75
63
51
39
27
15
3
28.1
43.8
44.7
49.1
52.0
43.7
38.8
23.2
6.5
4.8
5.1
4.9
5.1
5.2
6.0
6.9
-------
TEMPERATURE, OXYGEN, AND CARBON DIOXIDE IN DIGESTER - AUG. 1, 1969
180
160
3140
120
Begun August 1, 1969
Initial Refuse Height =7.3 Feet
Raw Sewage Sludge Added
70" Above
47" Bottom
22"
8"
100
20
15
10
Time in Days
FIGURE 7
11
70" Above Bottom
52"
40"
27"
12
-------
These data further suggest that decomposition is not
uniform in the digester and that there is a need for periodically
mixing the refuse. Moisture contents of waste near the top and
bottom of the digester were below the generally recognized range
for good composting (40 - 651). It should be noted that generally
the pH varied inversely with moisture content.
d) Effect of Aeration Rate - August 20, September 4 and 16:
The effect of aeration rate on the composting process
was studied by varying the air supply and observing the temperature
and gas results during the usual 8 day digester detention time. The
study was made in the north half of the west digester. The first
step was to determine the output of the aeration blower with the
digester full of refuse. The air flow meter of the instrument console
was used as the basis for regulating the valves on the air supply ducts
to obtain a uniform air supply along the digester. The air supply was
about 3800 cubic feet per minute (cfm) for the north digester half or
about 540 cfm for each of the seven 20.67 feet long tanks. At the time
the study was made, the blower ran 16 minutes per hour on the north
half of the digester.
The third experiment was started on August 20 with an
almost normal air supply of 600 cfm per tank. The initial refuse
height was 6.7 feet. Therefore, the resulting aeration rate was
3.52 ft^ per hour per ft^ refuse. Raw sewage sludge was added to
-162-
-------
give an initial moisture content of 49%. Samples taken from an
elevation of five feet at 3 and 8 days contained 44% and 43% moisture,
respectively.
Figure 8 shows that the maximum temperature reached was
165°F; this occurred at 5 days. As usual, the oxygen and carbon
dioxide concentrations were determined just before the aeration blower
turned on. Oxygen concentrations were usually in excess of 15% while
carbon dioxide was usually less than 5%.
By adjusting the air duct valves, an excessive lair flow
amounting to 1.5 times normal was fed to the refuse for the fourth
experiment which was begun on September 4. The initial refuse height
was 7.2 feet and the air flow was 990 cfm per tank. The resulting
3 3
aeration rate was 5.32 ft per hour per ft of refuse.
The initial moisture content was 491. At 8 days the
moisture content was 52% at the 2 ft. and 8 ft. elevations. The mois-
ture data show that an increase in aeration rate does not dry out the
refuse. Evidently, moisture produced by the composting process will
replace any that is driven off provided the initial moisture is high
enought and the aeration rate is not too high.
Figure 9 shows that temperature at the 66 and 49 inch
levels in particular rose rapidly and leveled off at about 1.5 days.
Temperatures at other levels rose as expected. Oxygen and CO- concen-
trations stabilized in about a day as compared with 2-4 days in pre-
vious experiments.
-143-
-------
180
TEMPERATURE, OXYGEN, AND CARBON DIOXIDE IN DIGESTER - AUG. 20, 1969
160
ofa 140
— 0)
(0
M
-------
FIGURE (9
TEMPERATURE, OXYGEN, AND CARBON DIOXIDE IN DIGESTER - SEPT. 4, 1969
180 r
160
D
I
W
£
13
140
120
100
o,
20
15
ffl 10
83" Above Bottom
32"
66" *
49" °
14"
Begun September 4, 1969
Raw Sewage Sludge Added
Initial Refuse Height = 7.2 Feet
Aeration Rate =5.32 Ft^ Air Per Hour
Ft-* Refuse
O 56" Above Bottom
TIME IN DAYS
-1687-
-------
The fifth experiment was begun September 16 and had
an air supply of 0.5 normal. The initial refuse height was 6.3 ft.
with an airflow of 310 cfm per tank. An aeration rate of 1..90 ft
per hour per ft of refuse resulted. The moisture content at the
beginning was 46%.
Figure 10 shows that temperature development was; slow
but reached 167°F at 4.5 days. Oxygen and carbon dioxide levels
were maintained well.
On comparing the results of the three runs, several
things became evident. The oxygen demand of the refuse-sewage sludge
mix appears to be satisfied with all three of the aeration rates.
It should be noted that the raw sludge used in these experiments was
added at a rate of from 30 to 50 gallons per ton of refuse moistened
and contained only 1.5% solids. It is estimated that addition of 110
gallons per ton of a 6% solids sludge would be required to consume all
of the sludge produced by the City. The oxygen demand of this thick
sludge would be considerably more than that of the dilute sludge used
during these experiments.
Even though the sludge used in these experiments was
dilute, there were suggestions that sludge as contrasted with water
caused temperatures to rise at a faster rate and level off more quickly.
In experiment 3 (Figure 8) temperatures leveled off between the second
and third days; in experiment S (Figure 10) temperatures leveled off
-------
FIGURE W
TEMPERATURE, OXYGEN AND CARBON DIOXIDE IN DIGESTER - SEPT 16, 1969
180
160
CM
o
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H
3
4-)
140
EH 120
100
63 in.Above Gravel
45 m.
29 in.
15 in.
4 in.
Begun Sept. 16, 1969
Raw Sludge Added
Initial Refuse Height
Aeration Rate = 1.90
1 in.
6.3 Feet
Air Per Hour
Refuse
42 in.Above Gravel
30 in.
18 in.
6 in.
345
Time in Days
-------
between the third and fourth days. Sludge was used for both experi-
ments. By contrast, in experiment 1 (Figure 4) when no sludge was
used, temperatures did not level off before days 5 and 6. This
stimulated rise in temperature correlates well with the earlier
findings that sludge stimulates decomposition. An exception occur-
red in experiment 2 (Figure 7) in which sludge was added but tempera-
tures did not level off until days 5 or 6. However, in experiment 2
some unknown influence profoundly slowed and then increased the
temperature rise. It is doubtful that the moistening agent would
do this.
The 1.5 normal aeration rate resulted in faster tempera-
ture development at some levels than the normal and 0.5 normal rates.
The 1.5 normal aeration rate also made temperatures more uniform
throughout the mass than did the others. The faster development and
increased uniformity of temperature resulting from the increased
aeration rate indicate conditions for better decomposition. Whereas
it is not possible to increase the air supply without changing the
blower setup; it is possible to extend the aeration period. (This
was done in the next experiment;)
One interesting result of the temperature observations
was that little temperature rise was noted at the levels from the
bottom up to four inches. (See Figures 9 and 10.) This again in-
dicates the need for mixing the refuse sufficiently during the
-------
digestion process. It is expected that decomposition would progress
very slowly at these low temperatures. Also, temperatures below
122°P C50°C) are seldom considered high enough to kill pathogens
within a practical length, of time.
On the other hand, temperatures attained throughout most
of the digester after 2-3 days are far too high for optimum microbial
activity. Temperatures above 131°F (55°C) limit microbial activity
to only the thermophilic types. The optimum temperature range for
growth of thermophiles is 131° - 140°F (55° - 60°C). The maximum
temperature at which growth occurs is 167°F (75°C). Very frequently
digesters operate near this temperature. This is not to say that
the digesters should be operated at lower temperatures. High tempera-
tures certainly favor rapid decomposition. Thus, it is strongly in-
dicated that decomposition above 167°F is not a result of microbial
activity.
e) Effect of Extending Aeration Time - October 27: The three
previous experiments on refuse aeration have indicated that a higher
aeration rate produced conditions for better decomposition. Those
experiments were conducted when dilute sewage sludge was added. The
addition of thick sludge was started on a regular basis in mid-October.
The aeration period for the digesters was extended in anticipation of
the increased oxygen demand of the thick sludge. It was extended from
16 minutes per hour to 28 minutes per hour in the test half of the
digester.
-------
An experiment designed to determine the effect of the extended
aeration time on refuse decomposition was begun October 27. The study
was to have run for an 8 day period. Unfortunately, the refuse was
removed accidentally after 3 days and much of the value of the experi-
ment was lost. However, those data that were collected were meaningful.
Thick sewage sludge was added to the refuse under study at a
rate of 110 gallons per ton. Sludge solids ranged from 6 to 7%. The
initial moisture content was about 551. Air supply to the section of
the digester containing the refuse under study was 610 cfm for 28
minutes per hour. The initial refuse height was 7.0 feet. The result-
ing aeration rate was 5.9 ft per hour per ft2* of refuse.
Figure 11 shows that temperatures rose quickly and that they
were uniform throughout most of the depth. Temperatures were still
increasing at all levels when the probe was withdrawn after 3 days.
Refuse temperatures at 20 inches and above exceeded 160°F on the third
day. These extraordinary temperatures gave a strong indication that
decomposition was accelerated by the increased air supply and/or by
the thick sludge.
The high oxygen demand of the refuse-thick sludge mixture was
shown by the results of the gas measurements. Oxygen levels dropped
sharply during the first 3 hours in the digester. The oxgyen con-
centration at the 75 inch elevation was 3.5% after 3 hours. The high
concentration of sludge was evidently responsible for the rapid oxygen
uptake. Oxygen concentrations recovered somewhat after the initial
drop but they were far lower than normal despite the high aeration
-170-
-------
180 1
160 '
"\
I
1
s
120 '
100
20 '
FIGURE 5,1
TEMPERATURE, OXYGEN, AND CARBON DIOXIDE IN DIGESTER - OCTOBER 27, 1969
O 68" Above Bottom
A 54,.
• 36"
B 20" " "
Begun October 27, 1969
Raw Sewage Sludge Added
Initial Refuse Height = 7.0 Ft.
Aeration Rate = 5.9 Ft-* Air Per Hour
Ft3 Refuse
O 75" Above Bottom
A 56"
• 38" " "
O 75" Above Bottom
A 56"
• 38"
TIM; IN DAYS
1-11
-------
rate. In all previous experiments, the oxygen concentrations ranged
from 16 - 19% after the initial drop. In this experiment, the average
oxygen concentration was about 9%.
Carbon dioxide production was high during the first 3
hours. A 002 concentration of 19% was measured at the 75 inch ele-
vation after 3 hours. On the third day, C09 concentrations at all
&
measured elevations dropped to about 8%.
This experiment shows the advisability of extending the
aeration period for the treatment of the thick sewage sludge supple-
ment. It appears that oxygen levels would have been quite low in the
digester if the previous aeration period had been retained and thick
sludge added. Anaerobic conditions might have developed and produced
foul odors.
8. Curing of Compost
Although the mechanical methods of composting are
considered accelerated methods, the effluents from these systems are
not sufficiently decomposed to be used directly for most agricultural
purposes. A curing period is required during which time the waste
decomposes to a relatively stable product.
Compost was cured in large storage piles at the Gaines-
ville Compost Plant principally because of a space shortage. It has
been observed that whenever these large piles were disturbed, a strong
sour odor evolved. The odor was indicative of acid accumulations which
develop under anaerobic conditions of decomposition. Anaerobic
-172r
-------
decomposition is slow and inefficient, and curing must proceed for
a long time before the compost is suitable for agricultural uses.
One of the large curing piles was observed for indica-
tions of decomposition. Samples were taken from several locations
within the pile and analyzed for moisture, pH, and BOD. Just prior
to sampling, a large quantity of compost was removed, thus exposing
a cross section of the pile. Samples were taken from this newly
exposed surface.
Figure 12 is a diagramatic sketch of the curing pile.
The results of the analyses and the estimated age of the compost
are given for each layer. The uppermost layer was a dry crust of
about 6 inches deep; all other layers were approximately 7 ft. deep.
The second layer was comprised of normal compost at the top and
charred compost at the bottom. The charred material resulted from
spontaneous combustion sometime before sampling. The zone of 12
month old compost was comprised of some of the first compost pro-
duced at the plant. Temperatures of the various zones were not
taken. However, it was noticed that they were high and almost
uniform except at the uppermost layer which was cool. The moisture
content of most layers was too low for curing. Similarly, the acid
conditions in the bottom half of the pile were unfavorable for cur-
ing and were strongly indicative of anaerobic conditions. That
curing had progressed further at the top of the pile than at the
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bottom is not indicated by the BOD data. Furthermore, BOD values
in the range of 40 - 75 mg/g are typical of compost treated only
by digestion. Thus, it appeared that most of the compost had not
cured despite the long storage time and the high temperature of
the pile. It follows, therefore, that uncured compost should de-
compose when subjectied to favorable conditions.
To test this hypothesis, some old anaerobic compost was
tested for stability by an established practical method. It is known
that unstable compost will decompose when it is moistened and sub-
jected to aerobic conditions. The course of decomposition can be
followed by observing the temperature of the pile which can be ex-
pected to rise at first, and then fall as the compost is either stabi-
lized or dried. Some old unstable compost was windrowed to attain
satisfactory conditions for decomposition. Six cubic yards of the
most cured compost available (judged to be about 1 year old) was
spread in a thin layer to cool. It rained on the second and third
days raising the moisture content to 47.7 percent. On the fifth day,
the compost was formed into a cone shaped pile and temperatures were
measured in numerous locations. Temperatures ranged from about 115 -
122°F (46 - 50°C). The probe was then placed in its permanent position
of 2 - 3 feet below the apex in the center of the pile.
Temperature data are given in Figure 13. The probe stabi-
lized shortly before noon during the first day of curing and the timing
-175*-:
-------
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of the experiment was begun. Each daily interval in Figure 2 repre-
sents the noon temperature and the midpoint between each day indicates
the midnight temperature. During the first 4 days the temperature
rose continually, but the rate of increase was slightly higher during
the day than at night. Thereafter, temperatures rose during the day
and dropped during the night. These diurnal fluctuations obviously
resulted from ambient influences. The waste decomposed at a rapid
rate for the first 4 days, at a lesser rate to approximately 11 days,
and then at a declining rate.
On the nineteenth day, the moisture had dropped to 26.2
percent. The waste was remoistened to 42.8 percent, aerated by lifting
it with the front-end loader and dropping it repeatedly, and shaped into
a pile. Within minutes, the temperature started to rise and within 48
hours it surpassed the previous maximum. These temperature data clearly
show that old anaerobic compost was not cured, and that it was decom-
posable when subsequently subjected to suitable conditions. These
findings suggested further work to determine the extent of curing of
freshly digested compost treated under aerobic conditions.
Two experiments were conducted to determine the extent of
treatment attained during the windrow curing of fresh compost. Water
had been used to moisten the waste in the normal operation of the plant
at the time the work was done. The windrows constructed for this work
were not turned or otherwise aerated.
-177-
-------
In experiment 1, effluent compost was contained in
Fiberglas bags which were deposited within a pile of compost con-
structed for exclusive experimental use. The pile consisted of
effluent compost of the same age as the sample. It was sufficiently
large so as to simulate actual production conditions in a windrow.
A subsample was prepared for immediate analysis. Three other sub-
samples were placed about 18 inches beneath the surface where they
remained hot and moist. Samples were removed from the pile after
6, 15, and 94 days of curing. Experiment 2 was refined somewhat
to provide comparative data on raw waste, waste composted in the
digester for 10 days and waste cured in a pile for 3 months. All
three subsamples were derived from the same well-mixed batch of
waste.
Table 6 clearly indicates that plant effluent was
highly unstable and that it continued to decompose during curing.
At 3 months when experiment 2 was terminated, triplicate samples
contained 29.4, 31.7 and 35.3 percent moisture. Further decompo-
sition may have been inhibited by drying.
It must be emphasized that the Fiberglas bags were
placed near the surface of the curing windrows and, therefore, were
subjected to optimum conditions for decomposition in an unturned
windrow. When both of the most cured samples were removed from the
windrows, it was observed that only a layer of compost extending from
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2 inches below the surface to about 20 inches deep was warm and
moist. The compost at the very surface and below 20 inches was
cool, dry, and looked poorly decomposed. Therefore, the extent
of curing observed in these experiments represents something near
the maximum attainable, after 90 days of treatment in aerobic
windrows.
This preliminary work did show the unstable nature of
effluent compost. Further work was conducted to quantify decompo-
sition in the digester and in subsequent curing in windrows. The
objective of this work was to quantify the amount of decomposition
accomplished in the digester and during subsequent windrow curing.
The second objective was to compare the rates of curing in a well-
managed windrow with that in an unmanaged windrow. The comparison
was made because production compost was cured in windrows during
one period of time. But, because turning and moistening equipment
was not available for production purposes, the windrows were riot
cared for. This work should demonstrate the results of managing
windrows.
The experimental plan was to follow refuse degradation
from the time the refuse was put into the digester until it had been
treated in a windrow for a period of two months. The temperature,
oxygen and CO- concentrations in the refuse were monitored through
much of the study along with the physical and chemical parameters.
180
-------
A sample of the raw refuse was taken as it entered
the digester. Part of the sample was analyzed and the rest was put
into 6 Fiberglas bags and placed in the digester. The samples in
the bags were removed as required for analysis after 3 and 8 days
in the digester and after 1 and 2 months of windrow curing.
Two windrows were constructed to represent two sets
of curing conditions. One of the windrows was turned and moistened
as required. The other was not turned or moistened.
Figure 8 shows the temperatures, oxygen and CO- concen-
trations, and aeration rates in the digester. This part of the work
coincided with the normal aeration rate study (Section H-7) and is
described therein.
Table 7 shows the chemical analyses of the samples of
raw and digested waste. The carbon content of the refuse dropped
211, whereas the nitrogen content dropped 13%. The resulting C/N
ratio reduction was about 9% for the 8 days in the digester. The
net BOD reduction was 10% after 3 days and 55% after 8 days. COD
and volatile solids values also dropped during the 8 day digestion
period.
The original plan of the experiment was to follow the
same refuse through the digester and windrow curing. Unfortunately,
an equipment breakdown delayed the removal of the refuse under study.
The experimental plan was to have the refuse in the digester only for
-181-
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the typical 8 day detention time. The refuse sample bags were
removed at 8 days and put into 2 windrows built with ground re-
fuse that had just come out of the digester after 10 days deten-
tion.
Windrow #1 was made with compost as received from
the digester and had an initial moisture content of 30%. Windrow
#2 was first moistened to 50% before being built. Both windrows
were about 5 feet high. Two of the original digester sample bags
were placed in each windrow at an elevation of four feet. The
samples were not ground after treatment in the digester. A sample
of the compost from each windrow was taken as it was made. A zero-
time sample was analyzed and two sample bags were filled and placed
in each windrow at four feet elevation.
Table 7 shows that the BOD reduction for this material
after one month in the windrow was 27% for windrow #1, but only 1.5%
for windrow #2. After two months the BOD reduction was 66% for win-
drow #2. The COD and volatile solids results for windrow #2 both
show reductions after 2 months. Moisture was maintained well in the
windrow #2 sanple for 2 months. The sample in windrow #1 lost moisture
after the first month and dropped to 33.8% at 2 months.
Table 8 shows the results of analyses made of the material
from which the two windrows were built. By contrast, Table 7 shows
the analytical results of samples contained in Fiberglas bags.
-183-
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The need for sufficient moisture content for curing in
a windrow to occur is indicated by the results in windrows #1 and #2.
Windrow #1 had only 30% moisture when constructed and 28% one month
later. After two months the moisture had increased to 38%. The BOD
reduction was less than 1% during the first month and only 7% at the
end of two months. Windrow #2 moisture was about 50% during the
same time and produced a BOD reduction of 60% for the first month
and 80% by the end of two months. Moisture content was evidently the
reason for the difference in the BOD reduction.
Temperature, oxygen, and carbon dioxide were measured
in the windrows during the curing process. The results are shown
in Figure 14. The temperature and gas were measured in the center
of the windrows at elevations of 1, 2, 3, and 4 feet.
Temperatures were generally higher in windrow #1 through-
out the period of observation. This fact can be attributed directly
to the moisture content of each windrow. After a rapid temperature
rise at all levels, windrow #1 cooled to temperatures ranging from
125°F to 145°F at 20 days. These same temperatures were maintained
up to the latest readings made at 47 days. The hottest part of the
windrow during this period was the lower half.
The initial temperature build up in windrow #2 was less
than that in windrow #1. However, after 20 days, the temperature
in windrow #2 ranged from 125°F to 141°F which was about the same
-------
FIGURE
TEMPERATURE, OXYGEN, AND CARBON DIOXIDE IN TWO WINDROWS - Aug. 28, 1969.
Windrow #1
160
Windrow #2
Initial Moisture = 50%
Turned at 33 Days
Initial Moisture = 30%
4 Ft. Above Ground
0 10 20 30 40
50 60 0 10
Time in Days
20 30 40 50 60
-------
as in windrow #1. The interesting thing is that the upper part
of windrow #2 was the wannest at 20 days while the lower part of
windrow #1 was the warmest at 20 days.
Oxygen levels were consistently higher in windrow #1
than in windrow #2. Windrow #1 was mostly aerobic while windrow
#2 was mostly anaerobic.
Rainfall was 4.2 inches during the one week period
following the construction of the windrows. This heavy rain appar-
ently penetrated the windrows. The dry windrow (#1) was not moistened
much by the rain. Water was observed flowing from the base of the wet
windrow (#2) after the rains. It was felt that the turning of windrow
#2 to restore aerobic conditions would require too much time to be
practical. The sample bags and temperature and gas probes would have
to be removed and replaced each time the windrow was turned. The con-
ditions in windrow #2 where the sample bags were located were aerobic
and it was decided to leave the windrow as it was. The windrow was
turned once at 33 days, but no improvement in oxygen content was noti-
ced.
Foul odors conmon to anaerobic compost were observed upon
sampling the gas in windrow #2. Nevertheless, Table 8 shows that decom-
position was much more extensive when the waste contained 50% moisture
as compared with 30% moisture. This is shown by reductions in C/N
ratios, BOD and volatile solids values. The significance of higher
-187-
-------
temperatures resulting from the decomposition of refuse containing
only 30% moisture is not understood. Better decomposition at the
higher moisture content is also shown by the greater oxygen demand.
It would appear that this curing experiment came very close to
anaerobic decomposition. Apparently the best decomposition will
occur when the initial moisture content is less than 50% and/or the
windrow is turned frequently.
When it became known that compost was not being oared
in large storage piles, a site was acquired for the curing of compost
in windrows. The windrows were constructed by dumping compost from
the end of a moving truck. This compost had been digested for 6 -
8 days. Either water or digested sewage sludge had been used as
moistening agents prior to digestion. The resulting windrows were
about 5 feet high and about 7 feet wide at the base. No attempt was
made to turn or moisten these windrows. No attempt was made to con-
duct a controlled study of these windrows because this was done pre-
viously on special experimental windrows conveniently located near
the laboratory. However, they were observed periodically for about
1.5 years to estimate the results of unmanaged windrowing.
Flies or rodents were never seen at the windrow site
although there was a distinct green compost odor after each hearvy
rain.
A sample of compost was taken from within a 3 month
old windrow about 2 feet below the top surface. It had the following
-188-
-------
analysis: carbon - 31.1%, nitrogen - 0.595%, C/N ratio - 52.3,
BOD - 58.9 mg/g, COD - 7724 mg/g, volatile solids - 68.7%, moisture
content - 48.4%. These values are typical of compost which had
been digested for 8 days. Thus, it appears that after 3 months of
unmanaged windrowing the compost had not cured much. Temperatures
of curing compost were taken at various elevations in a 3 month old
and a 2 week old windrow. The following compilation shows the results:
TEMPERATURES AT STIPULATED ELEVATIONS, °F
Elevation, Ft. 3 Month Windrow 2 Week Windrow
1 172 180
2 167 176
3 158 176
4 140 167
Temperatures in the 2 week windrow are about the same as those devel-
oped in the digester. Even after three months the temperatures re-
mained sufficiently high to either kill or prohibit the growth of
pathogenic microorganisms.
Subsequently, a windrow estimated to be 9 - 10 months
old was observed. At a point 6 inches below the top surface, the
temperature was 99°F. At a point 18 inches below the surface, the
temperature was 102°F. The ambient temperature was 84°F. The
compost at the surface of the windrow was very dry and had a distinct
grey color. A very profuse growth of a yellow to cream colored fungus
-189-
-------
peimeated the compost from 1/2 in. to a depth of about 6 in. below
the entire surface. Below this there was no more visible fungal
growth and the compost was very wet and dark. A pleasant odor of
leaf mold was emitted when the surface of the windrow was disturbed.
Windrows of 14 - 15 months old were deminished to only
1 1/2 feet high from their original height of about 5 feet. The
color of the compost was dark black. It had a faint earth-like odor.
Most windrows of this age were covered with a very heavy plant growth
thought to be Bermuda grass.
From these observations, it appears that partially di-
gested compost can be cured in unmanaged windrows, if time and space
permit. The only apparent nuisance was an odor of green compost after
heavy rains. It must be recognized that results noted here may not
occur in other climates.
9. Bulk Density of Compost in Storage Pile
Digested, reground compost was stored in large piles
for a period of time. Some of the piles were as high as 35 feet and
it is likely that the compost at the bottom is highly compacted.
Information on the bulk density of compacted compost may be useful
to those considering the transporting of compost.
Samples were taken March 10, 1969, from the lower part
of the storage pile. Three samples were carefully collected in the
form of chunks and put in thin plastic bags. The weight and volume
of the samples were calculated.
-190*
-------
The volume measurement was made with the compost chunk
in the plastic bag. A 3500 ml. beaker was filled to the point of
over flowing. The excess air was removed from the sample bag with a
vacuum pump and the bag immersed in the beaker causing an amount of
water equal to the volume of the sample to be displaced from the
beaker.
The sample was removed, the beaker was again filled to
the point of overflowing, and the amount of water required noted.
The results follow:
Sample
Number
1
2
3
Weight,
grams
215.7
702.4
620.0
Volume
ml.
465
1406
1139
Bulk Density
Ib/yd3
780
836
920
The average value of bulk density was 845 lb/yd^. The
bulk density values varied about +_ 9 percent so the resulting value
is only approximate. The moisture content within the storage piles
has ranged from 30-45 percent. Somewhat greater bulk density
would be expected in the lower part of the storage pile because of
compaction.
10. Process Evaluation - Miscellaneous
Generally, the objective of evaluating the composting
process was to determine what was accomplished. When it was shown
that some part of the process was poor, an alternative was selected,
and then it was evaluated. The use of windrows for curing compost
-191-
-------
in place of the original storage pile is an example of the accom-
plishments of process evaluation. Various minor aspects of the
composting process were evaluated.
a) Effect of Refluxing on Waste Decomposition: Several
investigators have shown that the seeding of refuse with special
microbial cultures or various natural inocula such as animal
manures failed to enhance the decomposition of refuse. The return
of some freshly composted waste to a similar raw refuse might pro-
vide a population of microorganisms more specifically adapted to
the waste being treated than a population from another source.
Accordingly, decomposition might be enhanced or accelerated. This
hypothesis was tested in one experiment.
Approximately eight cubic feet of freshly ground raw
refuse was placed on a concrete slab and made as homogeneous as
possible by mixing with hand tools. About one-half of a cubic
foot was subsampled and prepared for analysis to characterize raw
refuse. A Fiberglas bag was filled with approximately one cubic
foot of raw waste and it was placed in the digester. Refuse which
had been composted for 10 days was blended with the remaining raw
refuse on the concrete slab. The mixture contained approximately
80 percent raw refuse and 20 percent compost. A subsample was
taken for analysis to characterize the blend before further treat-
ment. Two other Fiberglas bags were each filled with approximately
-------
one cubic foot of blend and were placed in the digester adjacent
to the bag of raw refuse. The three bags were left in the digester
for 13 days. Detailed results reported previously1- ' were very in-
conclusive. There is little to indicate that refluxing enhanced
the decomposition of refuse.
b) Nitrogen Leaching from Compost Piles: The leaching of
nitrogen compounds from piles of compost to the soil may result in
serious pollution of ground water. This is known to be a problem
in animal feed lots, for example. Nitrogen compounds leaching from
the source to the underlying soil are converted to nitrate compounds
vfaich are very mobile in the soil. Nitrates which are not assimu-
lated by living plants rapidly descend to the water table. Water
thus contaminated and consumed by infants may give rise to the
disease known as infant methemoglobinemia, which can be fatal.
The soil underneath the bulk storage pile was found to
be almost black in color suggesting a type of soil or possibly ground
water contamination. Compost had been stored in this pile for about
1.5 years. Soil samples were taken from two locations near the pile
and from two other places located at least 100 yards from the pile.
One sample was taken from each of the four locations about 2 feet
below the surface. Samples were analyzed in triplicate for Kjeldahl
nitrogen by the same method used for refuse and compost. The follow-
ing compilation shows the average Kjeldahl nitrogen contents of each
of four soil samples:
193
-------
KJELDAHL NITROGEN, %
Near Storage Pile 100 Yards from Pile
.0407 .0382
.0534 .0153
Although this was not an exhaustive study of contamin-
ation from compost piles, it does suggest a potential for nitrate
contamination of ground water from improperly stored compost. It
is likely that compost stored in windrows would absorb more rain
water and thus reduce the leaching of nitrogen from compost.
c) Total Bacterial Counts: Numbers of bacteria in refuse
as a function of treatment time might indicate the rate of refuse
decomposition. It can be expected that the number of bacteria
will increase with time of treatment.
Bacterial counts were made on raw ground refuse and
periodically on the same refuse as it was being composted. Counts
were made of the bacterial population which decompose refuse rather
than those which are pathogenic as was done in another section of
this report. Approximately one cubic foot of raw ground refuse
was obtained for this study. Two grams of this refuse was commi*-
nuted with 200 ml of phosphate buffer in a Waring blender for 5
minutes. The buffer consisted of 1 g each of K2HPO, and KH-PO.
per 1000 ml of distilled water. The suspension was diluted and
plated on Bacto Tryptone Glucose Extract Agar (TGEA). This agar
-194-
-------
is not selective and therefore a great number of bacterial types
will grow on it The agar plates were put into a 99°F (37°C) incu-
bator for 24 hours after which the colonies were counted.
The original quantity of refuse was placed in a Fiberglas
bag which in turn was placed in the digester about 2 ft. below the
surface of the refuse. The refuse was sampled periodically, plated
as described above, and returned to the digester for further treat-
ment. Plates made from raw refuse and refuse composted for one-
half and one day were put in a 99°F incubator. Plates made from
subsequent samples were placed in a 122°F (50°C) incubator. These
temperatures generally correspond with those in the digester as
refuse digestion progresses. Thus, the general mesophilic popu-
lation was determined on the untreated refuse and that which was
composted for one day. The general thermophilic population was
determined on refuse which had been composted for more than one
day. The following compilation shows that the general bacterial
population decreased with increased duration of composting.
BACTERIAL POPULATION OF COMPOSTING REFUSE
IXiration of Composting, Days Numbers of Bacteria per g, dry wt.
0 3.4 x 10?
0.5 2.5 x 107
1 1.7 x 107
3.5 1.8 x 106
4 2.6 x 105
5 2.9 x 105
6 2.0 x 105
7.5 2.0 x 104
195
-------
The significance of these results is not known. Further
work is needed before it can be concluded that the number of bacteria
does actually decrease with the duration of composting. It is possible
that the high temperatures developed in the digesters may have reduced
the numbers of organisms which were decomposing refuse. It is gen-
erally known that a temperature of 131°F (55°C) restricts life to only
a few forms of thermophilic bacteria and fungi. Temperatures in ex-
cess of 131°F greatly deminishes the activity of these thermophiles.
Biological activity above approximately 140°F (60°C) is not known.
Nevertheless, composting is frequently accomplished at 160-180°F (72-
82°C). This implies a conversion from a biological process to a
chemical auto-oxidation process.
11. Arthropod and Rodent Control
Control programs for arthropods and rodents are greatly
dependent on the animal's behavior and ecology. Based on an sinimal's
habits, seasonal population changes, and life cycles, a practical
method of control may be devised. Such a study was undertaken to deter-
mine these and other ecological characteristics of the fly population
associated with the Gainesville Compost Plant. The fly population
was selected for this investigation because flies are present in
large numbers and are a short flight away from neighboring homes. Ro-
dents and other arthropods are insignificant in numbers and are easily
controlled with pesticides.
-196-
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a) Immature Flies; Fly larvae are present in incoming refuse
in considerable numbers during the warmer months. Many of these larvae
migrate from the refuse stored in the receiving area and cause annoy-
ance to workers, particularly those in the maintenance shop located
next to the receiving area. Other larvae reach protected areas in which
they transform into adults. A larval sampling program was conducted
from January - November, 1969. The objective of this program was to
develop procedures for reducing the larvae population and the ensuing
adult flies. To accomplish this objective, larvae which were brought
to the plant were identified as to species and the larval population
was characterized as to species composition. In addition, a population
factor was developed. This factor is a mathematical expression which
might be used for the estimation of the size of the incoming larval
population. It is based on sampling a known fraction of the incoming
population in a one-foot-square box-like container. The work described
in this report shows how the total incoming larval population was deter-
mined and how the population factor was used to estimate this population.
Table 9 shows larval types and counts from incoming refuse during the
period of January - November, 1969.
In order to determine the credibility and value of the
population factor, it was necessary to establish the percentage of the
total population that was trapped in the larval sampling program and
the reliability of this procedure. Visual observations indicated that
-------
TABLE 9
FLY TARVAE FOUND IN INCOMING REFUSE
Species
Percent Examined
Week Of:
Jan.
Feb.
March
April
May
June
July
Aug.
Sept.
Oct.
Nov.
12
19
26
2
9
16
23
2
9
16
23
30
6
13
20
27
4
11
18
25
1
8
6**
13
20
27
3
10
17
24
31
7
14
21
28
5
12
19
26
2
9
16
No.
Caught
0
4
2
0
0
1
0
0
0
0
12
130
102
78
131
285
614
1297
592
1629
2004
4554
3945
4387
4500
4482
3366
5669
5749
3534
4350
6116
4156
2621
1611
3597
3371
1638
620
212
173
18
No.
Examined
0
2
0
0
0
1
0
0
0
0
1
48
21
55
10
34
99
503
195
1013
330
697
1119
1543
703
1148
247
1041
2269
308
986
899
146
553
232
212
160
250
89
43
17
12
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closed for repair June 15-30.
-198
100.0
91.5
90.5
96.5
100.0
91.0
95.0
97.5
94.5
96.0
96.5
97.0
63.4
93.5
96.5
96.6
96.0
98.4
99.2
98.7
98.3
97.8
94.0
98.8
92.5
99.0
97.5
94.7
97.8
93.0
100.0
100.0
4.2
4.7
1.8
1.0
.4
.1
.9
.1
.4
.1
.9
1.2
.1
.1
.2
.1
1.3
.2
.7
.4
1.1
2.3
2.0
1.2
.6
1.0
7.5
6.0
1.6
1.9
1.2
.7
.1
.7
.5
1.3
.3
4.7
2.8
4.2
4.7
1.8
6.4
3.0
2.0
1.8
1.5
1.5
28.2
.3
1.8
1.5
.8
.2
.1
.2
.8
1.3
.2
.4
.5
.7
1.6
3.2
1.0
2.2
1.5
.9
.6
.3
.4
.1
.4
.4
.5
.6
.8
1.3
2.4
.3
1.7
.5
.7
.4
1.1
4.7
-------
the majority of larvae escaping from the refuse were confined to the
partially enclosed area under the apron conveyor. By sweeping this
area daily, it was possible to collect and estimate the number of
larvae migrating into this region each day. The number of larvae was
calculated by taking a random sample from the sweepings, counting the
number per sample, and computing the total number of larvae collected
on the basis of the sample size and the amount of sweepings. The
precision of this method was 6.00 +_0.2 larvae per gram when 20.0 Kg
of sweepings were sampled. This means that this counting procedure
was quite precise. Table 10 shows the calculated number of larvae
collected daily by sweeping under the apron conveyor, and also the
number of larvae trapped in the larval sampling program for that same
day. These values are then compared to determine the ratio of number
trapped to the total number of larvae present. These ratios are re-
corded as percent trapped in Table 10 . The mean of these ratios was
used to determine the population factor. This factor could be multi-
plied by the number of larvae trapped per day in the larval sampling
program to estimate the total number of larvae escaping under the
apron conveyor for that day. A mean of the percent trapped of 0.996%
was calculated and recorded in Table 10 . A standard deviation of
+0.284 indicates this factor is reasonably reliable.
Larval migration from a pile of refuse could be expected
to occur randomly in all directions. However, the construction of the
-------
TABLE 10
DETERMINATION OF POPULATION ESTIMATOR
Sweepings
(Kg)
19.05
27.0
1.80
18.4
10.7
14.5
9.82
17.4
16.3
22.2
Sample
(Gm)
976
1894
200
642
1076
1009
673
862
611
885
Larvae/
Sample
1741
2676
1500
3842
6855
4873
1993
1758
1796
3979
Number
Larvae
Collected
34,100
38,200
13,500
109,500
67,980
70,250
29,100
35,731
45,851
97,600
Number
Larvae
Trapped
487
452
114
986
297
602
238
411
490
1294
Percent
Trapped
1.41
1.17
0.84
0.89
0.44
0.86
0.82
1.15
1.07
1.31
I = 0.996 ± .284
-------
receiving area and the practice of refuse handling influenced larval
migration. Refuse is depoisted toward the east wall for storage during
peak loading and is progressively removed from west to east by the
front-end loader. Therefore, larvae migrating to the west are scraped
into the receiving hopper and they do not survive. Likewise, north-
erly migration results in no survival since the ramp and paved areas
provide no protective areas for pupation. Migration in an easterly
direction provides protection and larvae occur in large numbers along
the east wall of the receiving building. A wooden retaining wall,
which is approximately three feet from the outer wall of the building,
provides harborage to these migrating larvae. This area consequently
produces many adult flies. It was difficult to sample and the number
of larvae present was an approximation based on visual observations
and numbers collected under the apron conveyor. This factor was esti-
mated to be one-third of those under the apron conveyor for a given day.
From Table 10, it appears that in the larval sampling pro-
gram approximately one percent of the larvae migrating into the apron
conveyor area were trapped. Combining this factor with that calculated
for the number of larvae migrating under the east wall of the receiving
area, a population factor of 133 was obtained. This factor, when
multiplied by the daily larval catch, should give some approximation
of the number of larvae migrating from the refuse into the protected
areas of the plant. For example, Table 9 shows that 6, 116 larvae
-------
were trapped the week of September 7. Multiplication by 133 gives
an approximation of 813,400 larvae entering the plant during that one
week period.
It appears that refuse should be cleared from the receiving
area as quickly as possible to minimize the number of larvae which
could otherwise migrate from it. It is expected that the number of
larvae migrating from refuse is dependent in part on the length of
time a pile of refuse remains in the receiving area. This hypothesis
was tested by comparing the number of larvae collected under the apron
conveyor during plant working hours with the number collected in the
same area during off hours. This area was swept at the end of each
working day and again just prior to the next working period. The
number of larvae was calculated as previously described. Table 11
gives these results. The number of larvae collected during off hours
when compared to the total number of larvae per day reveals that a
reduction of more than one-third of the total larval population could
be accomplished by clearing the receiving area of refuse at the end
of each day.
Quite often refuse must remain in the receiving area and on
the approach ramp for several days. When this occurs the number of
migrating larvae increases considerably. These larvae migrate from
the refuse falling from the ramp to the pavement below. On several
occasions the number of larvae was so great that the pavement appeared
white. On one such occasion, the pavement was swept clean and the
larvae collected twelve hours later. Their number was estimated
to be 60,000 larvae per day migrating from the ramp (east) alone.
-202-
-------
TABLE 11
DETERMINATION OF NUMBER OF LARVAE ENTERING PLANT
DURING OFF HOURS
Sweepings
(Kg)
7.22
2.95
11.35
3.18
7.95
1.85
16.8
0.65
16.3
3.35
19.06
2.57
Sample
(Gm)
725
351
666
343
360
313
722
140
611
283
602
282
Larvae
Per
Sample
3400
3612
2508
2971
794
1961
1318
995
1456
2914
1992
2834
Time*
Interval
D
N
D
N
D
N
D
N
D
N
D
N
Number
Larvae
Collected
36,180
31,800
42,700
27,550
17,500
11,600
30,700
4,620
44,400
34,500
63,200
25,800
Total
Daily
Catch
67,980
70,250
29,100
35,320
78,900
88,900
Night Catch
Total Daily Catch
(%)
46.8
39.2
39.8
13.0
43.7
29.1
* D = 7:00 am - 6:15 pm
N = 6:15 pm - 7:00 am
I = 35.7 ± 12.4
-2S03-
-------
The majority of larvae that migrate from the refuse are third
instars and thus would require little external influence to develop
into adults. This was demonstrated by a series of hatching studies.
Larvae were placed into empty cups and other cups containing 25 gm
of refuse debris. Both types contained 100 larvae each. The cups
were covered with cloth, secured with a rubber band, and placed under
the apron conveyor. Adult flies emerged in 8 - 10 days. Nine repli-
cations of each test gave a mean of 65.31 adult emergence from the
cups with nothing added, and a mean of 88.8% adult emergence from the
cups with debris. These data indicate that a minimum of 65% of the
larvae which escape into the plant will emerge as adults and that if
these larvae migrate to the large amount of debris present under the
apron conveyor as many as 88.8% may be expected to emerge as adults.
Thus, it is concluded that refuse should be cleared from the receiving
area as quickly as possible. To do otherwise will encourage a fly
problem.
b) Adult House Flies: It was initially assumed that the
adult house fly population associated with the Compost Plant could
be estimated by sampling the fly population above the digesters.
A one-year study was initiated in January, 1969, to test the assump-
tion. The methods and some results were given in the last interim
report^6).
-20k-
-------
The survey showed that the fly population of the plant cannot
be estimated from the fly population of the digesters. Figure 15
shows that the numbers of house flies do not always correlate with
ambient temperatures. A sharp increase is recorded for the week of
May 4. This may be partially attributed to the high moisture content
of the compost that week. The plant was closed for repair the last
three weeks of June and no samples could be taken. Upon resumption
of operations, a sharp reduction was observed in numbers of flies
caught. These low numbers continued well until the latter part of
September when the daily catch began to increase. It is assumed
that the low catch during the summer is due to high temperatures
in the digester building where the air temperature often approached
110°F. Table 12 gives the average air temperature recorded on a
hydrothermograph placed above the digesters for several one week
periods. These data demonstrate more clearly the increasing fly
population with decreasing average hourly temperature.
A large number of house flies was observed around the
receiving area during the period of high indoor temperatures. This
further demonstrates the unreliability of this sampling procedure
during the summer months for estimating the total adult house fly
population. However, the procedure does show population trends in
the digester building. It may be useful in studies of fly breeding
in the digester.
-205-
-------
+AVERAGE WEEKLY LOW AMBIENT TEMPERATURE (F°)
1^
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AVERAGE NO. PEE YLK RIBBON
-206-
-------
TABLE 12
EFFECT OF TEMPERATURE ON NUMBER OF ADULT HOUSE FLIES
IN THE DIGESTER BUILDING
Air Temperature Of Digester Building
Average Average Average Hourly
Week Of: Daily High Daily Low Temperature
Average
No. Flies
Per Ribbon
August 17
September 21
October 19
November 16
106.4
99.4
99.1
92.7
93.6
90.3
88.8
75.6
98.8
94.9
94.1
83.5
9.0
9.9
13.0
77.3
-207-
-------
c) Adult Fly Control: An evaluation of several adult fly
control procedures was undertaken during the summer of 1969. During
the summer months, the majority of adult flies were observed around
the receiving area and along the conveyor belt system, with relatively
few observed above the digesters and other areas of the plant. Sweep
net catches in these areas indicated the vast amjority of these flies
were Phaenicia species. They were considered a nuisance to plant
workers, in particular the pickers.
During, and 30 days prior to, this investigation, the plant's
fly and roach control programs were suspended so that insecticide
residuals would exert little or no effect on the tests. Six fly con-
trol procedures were evaluated. Another procedure was discussed but
not evaluated. Effectiveness of the control procedures was determined
by comparing fly counts made during the treatment period with counts
made during a prior period of no treatment. The duration of pre-
treatment control sampling was 7 days; subsequent control durations
were 3 days. Treatment periods were alternated with control periods.
A 2' x 2' x 3' screen cone trap was chosen to sample the popu-
lations. This method gave 24 hour sampling and therefore was not sub-
ject to hourly variations. Fish heads were used to bait the traps
because they were readily available and they attract most species of
flies associated with refuse in this geographical area. Each trap was
baited daily with one fish head which had "aged" for 24 hours at room
-208-
-------
temperature. Due to the operation of equipment during normal plant
operations, traps could not be placed in the receiving area or along
the conveyor belts. However, the area behind the receiving building
proved suitable and two traps were placed in this area. Counting of
flies was facilitated by placing the trap in a large plastic bag and
killing the flies with ethyl acetate. The daily catch was recorded
as a sum of the catches of the two traps.
Treatments and treatment procedures are described as follows:
Sugar Bait: A 0.5% D.D.V.P. sugar bait, obtained
commercially under the name Fly Bait, was applied at a rate of 400
grams per day. This bait was distributed along the conveyor belt
system and under the apron conveyor for seven days.
Remove Larvae: The number of adult flies could be re-
duced by removing or killing the larvae which escape into the plant
before they metamorphose to adults. The area under the apron con-
veyor was cleaned and swept daily to remove the larvae which had
entered this area. This area was chosen because the majority of
larvae enter the plant here and it is relatively easy to clean. The
other main entrance for larvae, the east wall of the receiving area,
was not cleaned because this area was not accessible. This method
of control has some advantages. Because no pesticides are employed,
there are no dangers involved in working with poisons and there is no
problem of insect resistance. Because the transformation of larvae
-209-
-------
to adults takes approximately ten days under the existing conditions,
the area would not need to be cleaned every day to reduce adult: popu-
lations. It may be assumed that a weekly cleaning would be just as
effective as daily cleaning.
Malt Bait: A 25% malt solution containing 1.0% D.D.V.P.
was included in this study because malt bait was reported in the
literature as being very effective at a Florida dump for the control
of Phaenicia. Fifty ml were applied daily at four locations along the
conveyor belts for a one-week period. This bait has several dis-
advantages: (1) it is not available commercially, but must be pre-
pared by the user; (2) it must be stored under refrigeration; (3) it
costs more than sugar bait; and (4) its syrupy consistency makes it
inconvenient to work with.
Fogging; Fogging is not a highly recommended procedure
for effective control of adult flies. However, because personnel at
tne Compost Plant at Johnson City, Tennessee, included fogging in
their fly control program, this method was investigated. Fogging has
several disadvantages: (1) it leaves no residue, thus killing only
a percentage of the adult flies present at the time of fogging; (2) a
high concentration of insecticide is necessary to kill adult flies,
5% for fogs as compared to 1 - 21 for liquids; (3) initial cost of
fogger; and (4) the necessity of trained personnel.
It was observed that the adult blowflies, Phaenicia, left
the plant buildings at dusk and roosted in the grass immediately
-210-
-------
surrounding the plant during the night. Because the flies were con-
centrated in this relatively small area, it was concluded that fogging
the area at night would give the greatest chance of success. A 5.0%
Fenthion in No. 2 fuel oil solution was distributed by a portable hot
air swing fogger for three successive nights.
Residual Spray-Dimethoate: Because dimethoate is a widely
used insecticide for the control of adult and immature house flies,
it was chosen for this investigation. A 10% dimethoate solution was
applied once with a hand sprayer at a rate of 2 gm/M to the grassy
areas surrounding the plant used as roosting sites for Phaenicia.
Residual Spray-Rabon: Rabon is an effective larvacide
for house flies. A 10% Rabon solution was applied at a rate of
2 gm/M^ in the same manner as described for dimethoate.
Kill Larvae: Killing larvae to reduce the number of
adult flies could best be accomplished by the application of a
chemical larvacide. Due to the large amounts of falling debris under
the apron conveyor, the application of a larvacide wouldjbe ineffec-
tive and, therefore, was not attempted in this study.
Table 13 shows the effectiveness of each control procedure.
The fly count reduction attributable to each treatment is obtained
by comparing the mean daily count during treatment with the mean
daily count during the previous control period. In addition to the
usual pretreatment control, post treatment counts were used for the
evaluation of the fogging and sprays to determine residual effects.
The results follow:
-211-
-------
TABLE 13
ADULT FLY COUOTS*
Day Control
1 2343
2 2796
3 2268
4 979
5 1050
6 3456
7 824
8
9 X = 1959
10
11
12
13
14
Sugar Bait1 Day
1
2
3
4
5
6
7
, 1178 8
875 9
285 10
596 11
1041 12
490 13
107 . 14
X = 653
Control Sweepin;
1197
2316
1954
856
X = 1822 1055
908
667
! 353
1061
1775
1493
1437
1315
988
X = 1083
0.51 D.D.V.P. in sugar, daily
Area under apron conveyor swept daily
/
Number of flies caught per day
-------
TABLE 13 (CONTINUED)
Day Control
1 1711
2 1397
3 . 'J985
4 % - 1698
5
6
7
8
9
10
Malt Bait?
360
514
641
1245
1467
597
1017
X= 831
Day
1
2
3
4
5
6
7
8
9
10
Control Fog^
1967
1949
1826
X" = 1914 . 561
661
561
627 X = 594
1581
1601
1811
X" = 1405
-1.0% D.D.V.P. in 25% Malt solution, daily
5.0% Fenthion in No. 2 fuel oil, days 4-6
-------
TABLE 13 (CONTINUED)
Day Control
1 1437
2 1719
3 2241
4 X = 1799
5
6
7
8
9
10
11
12
13
14
15
Dimethoateb
28
13
37
18
64
49 X = 46*
112
287
170
393
811
1256
X = 287
Day
1
2
3
4
5
6
7
8
9
10
Dimethoate @ 2 gms./M^, one application on day 4
Rabon @ 2 gms./M » one application on day 4
*
Mean of one week after treatment
Control Rabon^
1677
1170
1410
X = 1419 1056
653
1251
734
f- 949
781
1090
3C = 931
-------
Sugar Bait: This treatment reduced the adult fly popu-
lation by 66.7 percent. The cost of one daily application was about
$0.50 plus 0.1 man-hour.
Remove Larvae: This procedure reduced the adult fly
population by 40.5 percent. About 4 man-hours per week were required.
Malt Bait: This treatment reduced the population by 51
percent. The cost of one daily application was about $0.70 plus 0.1
man-hour.
Fogging: This treatment reduced the population by 69
percent. The cost of one fogging was about $5.00 plus one man-hour.
The post treatment counts show that the residual effect of fogging
is minimal after 1 day.
Residual Spray-Dimethoate: Dimethoate reduced the popu-
lation by 97.5 percent. The post treatment counts showed a residual
effect for more than 10 days after the single application. The cost
of one application was about $7.00 plus 0.5 man-hour.
Residual Spray-Rabon: This larvacide reduced the popu-
lation by 34.4 percent. The cost of Rabon treatment was approximately
the same as for dimethoate.
The study of adult fly control is not complete. Nevertheless
it does show that adult flies can be controlled around waste treat-
ment facilities. Furthermore, the study emphasized the need for
background knowledge, both general and specific for the given plant,
as a basis for fly control. Spraying with dimethoate resulted in
-215-
-------
the best fly control. The need for good housekeeping programs
was also shown. Although sweeping resulted in only 40 percent
reduction it must be emphasized that only a portion of the plant
was swept. Therefore, good housekeeping practice is probably
more beneficial than these data indicate.
d) Other Fly Studies: An investigation was undertaken to
determine the influence of moisture and extent of waste decompo-
sition on house fly breeding. In this study compost of various
ages was tested at different moisture contents to determine the
effects on rearing house flies. The compost used was taken from
the digesters between 12-24 inches from the surface. The de-
sired moistures were obtained by adding tap water. Fifty grams
(dry weight) of compost was placed in a one quart waxed cup with
100 eggs or 100 larvae (48 hours old). The cup was then covered
with a piece of cloth which was secured by a rubber band. CSMA
rearing medium at 66 percent moisture was used as a control.
Table 14 shows the number of pupae formed. Forty-
eight hour larvae will survive in compost of all ages and moisture
contents tested. House fly eggs survived best in 60 - 75% moisture.
The age of the compost also influenced survival. The final grind
reduced survival considerably but still showed 8.5% survival in the
finished product. Further tests are planned in this area. Another
test is planned in which the amount of sludge will be varied to
determine influence in breeding in the digesters and in the laboratory.
-------
TABLE 14
INFLUENCE OF MOISTURE AND AGE OF COMPOST ON HOUSE FLY SURVIVAL
•** Average Number of Pupae per 100 Larvae
3
-J
3
~!
~J . ;
3
1
1
T
"
1
1
-]
(48 Hours Old)
Time Composted — Percent Moisture
(Days) 30 45 60 75 90
0
1
3
5
10
10 */
0
1
3
5
10
10 I/
82.
59.
84.
76.
81.
82.
Average
0
0
0
0
0.
0
5 78.0
3 81.3
3 79.3
0 80.6
6 79.6
8 86.6
Number of
0.8
0
0
0
0
0
8-1.8
58.6
80.3
84.6
86.6
89.0
Pupae
16.5
1.3
21.2
3.1
3.1
0.3
81.6
33.5
86.3
91.6
90.3
90.5
per 100
43.3
13.8
40.8
39.0
25.1
8.5
67.0
50.3
80.. 1
70.1
58.5
80.3
Eggs
5.6
0
0.8
0.6
0.3
0
Control
99.1
92.5
90.1
92.6
92.6
92.6
80.6
87.6
78.6
69.8
80.5
88.3
I/ Length of time in digester tanks
J
* 2_/ After passing through final grind (Finished Product)
•w-
-------
A single test was performed to determine if larvae
entering the plant in the refuse could survive the grinding process.
Third instar larvae were passed through the grinder and some living
larvae were recovered. During this period the primary grinder was
not in operation and only the secondary grinder was in use. This
experiment cannot be interpreted quantitatively, but it does show
that some larvae will survive the grinding process. These larvae
may proceed to the digesters where they may develop if conditions
are suitable.
12. Public Health Aspects of Composting
The objective of the public health evaluation was to
delineate possible health hazards associated with high-rate compost-
ing processes. Potential health hazards are created by pathogenic
microorganisms in refuse and compost and those in aerosolized form
in work areas. Potential health hazards also are created by arthro-
pods and rodents which are attracted to wastes and are vectors of
diseases afflicting man and animals. Control of arthropods and ro-
dents was reported in Section H-ll. Additional hazards include non-
viable aerosolized matter which could cause lung diseases and noise
which could damage the hearing of plant personnel. Methods for the
assesment of public health hazards were presented in Sections E and F.
a) Microbiolpgica.1 Evaluation: The purpose of the microbio-
logical evaluation was to determine whether microorganisms associated
-------
with refuse and compost constitute health hazards, real or potential.
Health hazards to the consumer are inferred by the detection of certain
microorganisms "which indicate the presence of pathogens in the compost
product. Health hazards to plant personnel are manifested by physical
examinations and by the detection of pathogenic microorganisms in the
air breathed by workers. The public health quality of compost was
evaluated by periodic examination for fecal colif orms, Salmonellae,
and by special die-off studies.
Fecal coliform bacteria were used as indicators of
pathogenic microorganisms. The assumption was that the destruction
of coliforms by composting would also indicate the destruction of
pathogens. Consequently, the numbers of fecal coliforms in compost
was compared with the numbers of the same organism in raw refuse.
Table 15 shears the average fecal coliform indices in refuse and compost
over an extensive period of time. Coliform bacteria readily survived
in composting refuse. The length of treatment by composting varied
from 3-10 days. As discussed in Section H-6 the survival of coliforms
was more dependent on the moistening agent than on the length of treat-
ment. When water was used to moisten raw ground refuse, the coliform
population in the resulting compost remained stationary or increased
relative to the original population. By contrast, when sewage sludge
was used, the coliform population in the resulting compost decreased
for the most part.
-------
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Regardless of the effect of the moistening agent
the coliform bacteria survived the composting process. This might
indicate that pathogenic microorganisms also survive composting,
although this possibility was discounted in subsequent work.
Salmonella bacteria were also used as indicators
of pathogen destruction. Indication of the destruction of patho-
gens is based on the same principle as that in the coliform method
except that the Salmonellae were not determined quantitatively.
Samples of raw refuse and compost were examined for the presence of
Salmonella . A total of 144 samples of raw refuse, 101 samples of
plant effluent compost, and 2 samples of old stockpiled compost
were examined for the presence of Salmonella . This organism was
found in three samples of raw refuse and in two samples of compost
which was cured for an estimated duration of 1 year. It was never
found in samples of compost which was subjected to digestion only.
Digested sewage sludge was used for moistening refuse when Salmonella
was detected in two samples of refuse. Raw sewage sludge was used
when Salmonella was detected in one sample of refuse. No sludge
had been added to refuse when it was processed a year prior to sampl-
ing for Salmonella in the resulting old compost. The finding of
Salmonella in this old compost is inexplicable.
The meaningful use of Salmonella as an indicator of
pathogen destruction by the stipulated method is very doubtful
-22Z-
-------
inasmuch as it was seldom found in raw refuse. Thus, the failure to
find Salmonella in effluent compost does not indicate that it was
killed during the composting process. Its use in another method
whereby refuse is intentionally seeded with Salmonella could be mean-
ingful .
Composting, besides accomplishing a satisfactory amount of
waste stabilization, must also accomplish waste sanitation. 'This is
particularly important when refuse is amended with raw sewage sludge.
The destruction of pathogenic microorganisms would indicate that waste
has been sanitized. However, it is extremely difficult to detect
pathogenic organisms in solid wastes because of their presence in very
small numbers and because the presence of other microorganisms in
vast numbers obscures the pathogens. The detection of pathogens in
compost would show that they survived the composting process or
that they were reintroduced. Failure to detect pathogens in compost,
on the other hand does not necessarily show that they were killed.
Failure could mean that either the particular sample contained no
pathogens or if it did, they might have been overgrown by other
microorganisms during the duration of the test procedure. This
suggested the use of an assay procedure for the detection of micro-
organisms which might indicate the presence of pathogenic micro-
organisms. The selected assay procedures will detect the indicator
organisms in the presence of large numbers of extraneous organisms.
-122-
-------
However, it is questionable whether the indicator organisms truly
indicate the presence of pathogenic organisms. Furthermore, the
detection of coliforms and Salmonella as indicators had doubtful
meaning as discussed above.
Table 16 shows the plate counts before and after treatment for
the various organisms tested. In general, the results show that high-
rate composting has a pronounced adverse effect on the pathogenic bacteria
evaluated under this study. Escherichia coli, a non-pathogenic bacterium,
survived 12 days of composting. In only one experiment, in which E_. coli
was exposed for 4 days, was there any evidence that its population was
decreased. The precision of this counting procedure is usually one
order of magnitude. Thus, a population reduction in 12 days from
8 8
3.1 x 10 to 1.0 x 10 is not significant. It is interesting to note
that whereas the population of E_. coli remained stationary in 3 of 4
experiments, previous work showed that the numbers of coliforms were
reduced when refuse was supplemented with sewage sludge. The coTitorm
bacteria consists of a group of species of Escherichia, including E_. coli
and Aerobacter. Apparently, the reduction of coliforms is attributable
to the reduction of species other than E_. coli which remains approxi-
mately stationary.
Salmonella typhimurium was killed in one day when exposed to
composting refuse. When incubated in the laboratory at 99°F in the
-223-
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presence of ground refuse the population remained stationary. S_.
paratyphi was killed in one day if a temperature of approximately 131°F
is attained.
Bacillus stearothermophilus is one of the most heat tolerant
organisms known to exist. It is frequently used to test the operation
of autoclaves and other sterilizing equipment. Vegetative cells of
this organism were readily killed in one day in a composting environ-
ment, although a population of spores were not completely reduced in
two days.
On the basis of these data it appears that high rate composting
is likely to kill pathogenic organisms in two days. It must be realized
that both the temperature and the duration of time that organisms are
exposed to heat are important factors in producing thermal death.
Therefore, refuse which is not heated adequately will harbor living
pathogens. It was not an objective of this work to develop all in-
clusive time and temperature relationships for thermal death since this
has been investigated by others. However, it does appear that high rate
composting sanitizes refuse more quickly than windrow composing.
b) Airborne Particulate Matter: The air in dusty areas of
the plant was sampled for particulate matter as an indicator of poten-
tial public health hazards. Particulate matter was determined with
both a standard high volume sampler for total particulates and
with an Andersen Sampler for particle size distribution. Both viable
and non-viable particulate matter was determined with the Andersen
Sampler.
-226-
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An 8 x 10" Fiberglas filter was used in the high volume sampler
which is 99% effective in removing particles ranging in size from 0.1
micron and larger. The flow rate of air through the instrument was
39.2 cubic ft. per minute. The instrument was placed in the plant
midway between the primary and secondary grinders. It was operated
for 4 3/4 hrs. in the afternoon of October 17, 1969. Total particulate
•z
matter was 3793 micrograms per cubic meter of air (yg/m ). This value
is extremely high as compared with acceptable ambient air values
reported in Air Quality Criteria for Particulate Matter (issued February,
1969, by the National Air Pollution Control Administration). Adverse
health effects were noted when the annual mean level of particulate
matter exceeded 80 yg/m , visibility reduction to about 5 miles was
observed at 150 ug/m , and adverse effects on materials were observed
at an annual mean exceeding 60 yg/m .
Air was sampled for viable particles with a Model 0604 Ander-
sen Sampler (Andersen Samplers and Consulting Service, 1074 Ash
Avenue, Provo, Utah, 84601). The sampler was used at various areas
in the compost plant where suspended dust was obvious. The sampler
separated microorganisms into six categories according to size and
aerodynamic properties. The microorganisms were impinged on the
surfaces of agar contained in six Petri dishes. Tryptone-Glucose-
Extract Agar was used for the detection of general airborne micro-
organisms, Potato Dextrose Agar for fungi, and Brain-Heart Infusion
-227-
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Agar for pathogenic bacteria. The agar-impinged organisms were
incubated for 18 - 24 hours until small colonies developed, at
which time they were counted.
Numbers of viable particles collected from the air in
various areas on the plant premises are reported in Table 17. The
data clearly show that two areas in the plant contain large numbers
of aerosolized microorganisms at the time of sampling. By comparison,
the laboratory air was relatively clean. The data indicate the pre-
sence of broad groups of microorganisms such as bacteria and fungi,
but do not indicate pathogenicity. According to the manufacturer of
sampler, any particle 5 microns or less in diameter can penetrate the
lungs and is therefore hazardous.
Particle size distribution (weights of particles of various
sizes) was determined at two locations in the plant with the Anderson
sampler. Two determinations were made on September 9, 1969, at the
operators platform. Duration of sampling was one hour in the morning
and 30 minutes in the afternoon. One determination was made on
September 9, 1969, at the picking table. The duration of that
sampling was one hour. Particle weights were determined by weighing
the glass collection plates before and after exposure and by sub-
tracting the two values.
Table 18 shows that both plant areas are extremely dusty. It
is generally recognized that particles having a diameter of 5y or less
can penetrate the lungs. Lung penetrability increases as particle
-228-
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Agar for pathogenic bacteria. The agar-impinged organisms were
incubated for 18 - 24 hours until small colonies developed, at
which time they were counted.
Numbers of viable particles collected from the air in
various areas on the plant premises are reported in Table 17. The
data clearly show that two areas in the plant contain large numbers
of aerosolized microorganisms at the time of sampling. By comparison,
the laboratory air was relatively clean. The data indicate the pre-
sence of broad groups of microorganisms such as bacteria and fungi,
but do not indicate pathogenicity. According to the manufacturer of
sampler, any particle 5 microns or less in diameter can penetrate the
lungs and is therefore hazardous.
Particle size distribution (weights of particles of various
sizes) was determined at two locations in the plant with the Anderson
sampler. Two determinations were made on September 8, 1969, at the
operators platform. Duration of sampling was one hour in the morning
and 30 minutes in the afternoon. One determination was made on
September 9, 1969, at the picking table. The duration of that
sampling was one hour. Particle weights were determined by weighing
the glass collection plates before and after exposure and by sub-
tracting the two values.
Table 18 shows that both plant areas are extremely dusty. It
is generally recognized that particles having a diameter of 5y or less
can penetrate the lungs. Lung penetrability increases as particle
-229 -
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size decreases from 5y. Thus, particles collected on stages 3-6
are especially hazardous to exposed personnel. All values exceed the
air quality criteria set by the National Air Pollution Control Admin-
istration (NAPCA). Most values greatly exceed these criteria.
Even though only a few analyses were made, one can readily see
that the atmosphere near the grinders and the picking table is very
dusty. Thus, even though the reported values might be 2 - 3 tijnes
higher than typical values, a definite hazard is still manifested.
Data showing particulate matter in ambient air greatly exceed the
NAPCA air quality criteria for maximum tolerable levels. Therefore,
it is concluded that the atmosphere surrounding the primary and
secondary grinders, the operator's platform, and the picking table
is a potential hazard to the health and well-being of exposed
personnel.
c) Parasitological Analysis of Compost; The presence in
compost of viable eggs and mature forms of intestinal parasites
would be evidence of insufficiently sanitized compost. Samples of
sewage sludge, ground refuse, and compost were examined for intestinal
parasites. Samples were taken in October, 1968, and in January, March,
and October, 1968. They were sent to the Division of Research and
Development, Solid Waste Office for examination.
As expected, sewage sludge either raw or digested
contained intestinal parasites. Raw ground refuse was never found
to contain eggs or mature forms, but one sample of digested refuse
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which was not moistened with sludge did contain rhabditiform larvae
of hookworm or Strongyloides. Two ova of Hymenolepsis diminuta were
found in one sample of digested refuse which had been supplemented
with digested sludge. It must be emphasized that the analytical
methods used in the performance of this work could not be distinguished
between living and dead parasites. However, it is possible to distin-
guish between intact and decomposed organisms. It was assumed, there-
fore, that the intact organisms survived composting and decomposed
organisms did not.
It was strongly indicated that the curing of compost in
windrows killed intestinal parasites. Ova of Ascaris lumbricoides and
Hymenolepsis diminuta and hookworms or Strongyloides larvae were found
in compost which had cured in windrows for up to 3 months. However,
none of the windrows were turned or otherwise managed. Those parasites
that apparently survived 3 months of curing were found in compost which
was collected within 3 inches of the surface. Although it is not known
what effect windrow turning would have on parasite survival, it is
likely that survival time would be reduced.
d) Physical Examinations: All plant workmen and most admin-
istrative personnel were given periodic physical examinations to deter-
mine clinical manifestations associated with exposure to solid wastes.
The plan was to have each person examined before any exposure to
solid wastes and then periodically thereafter. Comparison of the
-233-
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findings before and after exposure might reveal certain health
hazards. However, rapid personnel turnover and inherent short-
comings of clinical studies made it difficult to make a good
comparison.
Seventeen employees were given complete physical exam-
inations. Twelve employees were reexamined about one year la.ter.
No changes were noted in their general state of health. Thirty
Mantoux skin tests were given; three were positive indicating that
they were exposed to tuberculosis. One of the positive reactors
gave a positive reaction prior to any exposure to solid wastes. It
is not known whether the other two positive reactors were previously
positive. All of the seventeen histoplasmin skin tests were negative.
The accident record attributable to solid waste handl-
ing has been good with two individual exceptions. Table 1$ shows the
frequency and types of injuries.
e) Noise; Sound level determinations were made on two occa-
sions in the vicinity of all pieces of heavy equipment in the plant.
The surveys were made when the Jay Centriblast shredder was in use
and then again after it was replaced with a Williams shredder. Detail-
ed results were given in two previous reports ^ ' . In summary, the
most hazardous piece of machinery regarding noise is the primary
shredder. The hazard results not only from the high level of noise
generated, but also from the close location of the laborers to this
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TABLE 19
SUMMARY OF WORK INJURIES
Date of
Injury
6/25/68
11/5/68
11/19/68
7/24/69
8/8/69
10/1/69
Time
Lost
158 days
39 days
3 days
1 day
1 day
1 day
Age of
Employee
40
21
47
17
33
19
Job
Classification
Maintenance
Grinder Oper-
ator
Welder
Salvage Labor-
er
Laborer
Truck Driver
Type of
Injury
Fractured bone
in arm
Cut tendons in
foot
Fractured bone
in toe
Twisted knee
Sprained ankel
Cut hand
Nineteen minor injuries such as cuts and bruises
No lost time.
Total days lost because of injury - 203
Total work days, approximate - 41,600
Percent of work time lost, approximate- 0.48
-235-
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source of noise. These laborers are exposed to sound levels of
100 - 102 dbA. The maximum exposure time to this intensity allowed
by the Safety and Health Standards for the Walsh - Healey Act is
1.5 - 2.0 hours per day. Because these laborers are exposed for
longer periods of time, they were issued ear plugs for their protec-
tion.
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REFERENCES
1. 1967 Interim report. Gainesville [Florida] Municipal Waste Conversion
Authority, Inc. (The information in references 1, 2, and 3 is
published in: Gainesville compost plant; an interim report.
Cincinnati, U.S. Department of Health, Education, and Welfare, 1969.
345 p.)
2. Interim report, January-April 1968. Gainesville Municipal Waste
Conversion Authority, Inc.
3. Interim report, May-August 1968. Gainesville Municipal Waste Conversion
Authority, Inc.
4. Interim report, September-December 1968. Gainesville Municipal Waste
Conversion Authority, Inc. Unpublished data.
5. Interim report, January-April 1969. Gainesville Municipal Waste
Conversion Authority, Inc. Unpublished data.
6. Interim report, May-December 1969. Gainesville Municipal Waste
Conversion Authority, Inc. Unpublished data.
7. Tentative methods of analysis of refuse and compost; appendix A. In
American Public Works Association. Municipal refuse disposal. 2d
ed. Chicago, Public Administration Service, 1966. p.375-399.
8. American Public Health Association, American Water Works Association,
and Water Pollution Control Federation. Standard methods for the
examination of water and wastewater; including bottom sediments and
sludges. 12th ed. New York, American Public Health Association,
Inc., 1965. 769 p.
9. Official methods of analysis. 10th ed. Washington, Association of
Official Agricultural Chemists, 1965. 957 p.
10. Willis, H. H. A simple levitation method for the detection of
hookworm ova. Medical Journal of Australia, 2:375-376, 1921.
11. Ritchie, L. S. An ether sedimentation technique for routine stool
examination. Bulletin of the U.S. Army Medical Department, 8:326-
330, 1948.
12. Jann, G. J., D. H. Howard, and A. J. Salle. Determination of
completion of composting. Applied Microbiology, 7:271-275, 1959.
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