PATHOGENS ASSOCIATED
   WITH SOLID WASTE PROCESSING


           A Progress Report
U.S. ENVIRONMENTAL PROTECTION AGENCY

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PATHOGENS ASSOCIATED WITH SOLID WASTE PROCESSING

                A Progress Report
    This publication (SW-49?) was written by

              MIRDZA L. PETERSON
      U.S. ENVIRONMENTAL PROTECTION AGENCY
                      1971
           ,830 £'L

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               PATHOGENS ASSOCIATED WITH SOLID WASTE PROCESSING

                             A Progress Report*


                                   Abstract


 t       Studies were made  to determine  the presence and  levels of microbial

 ,  pathogens associated with municipal  solid waste processing.  The efficacy
'\
'" '  of  several  incinerator  types to destroy bacteria associated with solid
 ?
   waste were  evaluated with emphasis on fecal and food wastes.  Microbiological

   sampling and analytical  procedures were developed and perfected.  Samples

   of  solid waste and  its  residue after  incineration,  taken  from eight

   incinerators of different design, were examined for (l) total bacterial

   cell number, (2) total  coliforms, (3) fecal coliforms,  (4) heat-resistant

   sporeformers,  (5) selected enteric pathogens.  Quench water was analyzed

   from  three  incinerators.  Survival of coliforms and enteric pathogens  in

   the residue after incineration was considered an indication of inadequate

   incinerator design or operation or both.

       Of the eight incinerators tested, only one produced  residue devoid of

   fecal coliforms; seven  others produced residue containing  fecal coliform

   populations of less than 1.0 x 101 to k.J x 103 per g.  Salmonella organisms
       "Progress  report on Project RS-03-68-16, Pathogens Associated with
  Solid Waste Disposal Processes.  The first  results of  this  investigation
  were published  by Applied Microbiology  in July  1969 and January  1970.
  The results of  other investigations will be published  in  the  International
  Journal of Environmental Studies,  (England).
                                     i i i

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were found in the quench water and in the quenched residue at one of the




eight incinerators tested.




     To measure microbial cell concentrations  in the  incinerator stack




emissions, a sampler was designed.  The measurements  showed that a few




viable gram-positive bacilli  (2 organisms per  cu ft air) were passing




through the stack of a conical burner, but that most  of the organisms




were scrubbed out.




     Quantitative studies were made on the microbial  flora of the dust




from six municipal incinerators, with particular emphasis on bacteria




of proved pathogenic!ty  for the respiratory and intestinal tracts and




the skin.  Three specific waste handling areas such as  the dumping floor,




the charging floor, and  the residue area were  tested.  Total microbial




cell counts in the three areas tested ranged from  1 to  197 organisms per




0.25 cu ft air.  Incinerator  dust was found to carry  Staphylocoeous




auveuSy D-iploooaous pneumonias, and Klebs'ielta pnevmoni>ae, although




these represented only a small percentage of the total microbial number.




Esdhevi,ohi,a col-i was isolated from the dust at five of  the six  incinerators




tested, and the dust from the. dumping and the  charging  floor areas of




all six incinerators contained a-hemolytic streptococcus.
                                  l v

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            PATHOGENS ASSOCIATED WITH SOLID WASTE PROCESSING




                            A Progress Report






                           Mirdza L. Peterson"






     Inefficient and improper methods of processing and disposal of solid




waste can create serious health hazards.1  Insects and rodents  in (or




associated with) waste a/e vectors of human disease; the housefly transmits




at least 30 bacterial, viral, and parasitic diseases, the rat has been




implicated in the spread of plague, murine typhus, leptospirosis, and




rickettsialpox.2-6  Prompt collection and proper disposal of solid wastes




are considered the most important factors in the control  of solid waste




related disease vectors.7




     The National Research Council indicated that solid wastes are




disposed of primarily by deposit on or below the surface of the ground




and through discharge into natural waters and the atmosphere.8  The currently




accepted methods for the disposal and volume reduction of municipal  solid




wastes are:  sanitary landfill ing, composting, and incineration followed




by landfill ing of the residue or unwanted material.9  Reports indicate




that (a) an improperly located or unsealed landfill will  leach soluble




salts and alkalies from the refuse and transport them to receiving waters,
     "Research Microbiologist,  U.S. Environmental Protection Agency.

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(b) a wide variety of pathogenic fungi can survive the composting process,




and (c) incineration byproducts have public health associated implications.10-12




     The Federal program in solid waste management, formerly in the




U.S. Public Health Service and now in the U.S. Environmental Protection




Agency, sets forth national goals that would assure that man's health and




quality of his environment are not endangered or degraded.  Particular




research objectives to meet these goals were selected and carried out by




the research and development division of the Solid Waste Management Office.




One specific objective was to establish the microbiological quality of




the total  effluent from solid waste incineration and to suggest ways of




improving operations of current and to-be-developed incineration processes.




     The division of research and development conducted laboratory and




field studies to evaluate  incinerator operations from the microbiological




viewpoint.  The purpose was to ascertain whether such operations provide




conditions with the capability of destroying refuse-associated pathogenic




microorganisms.  Attention was directed to two groups of organisms:  those




proved in the past to be pathogenic to man and those pollution indicators




useful in determining the potential presence of pathogens and their




survival after  incineration.  Refuse from food sources and fecal wastes




found at the incinerator storage pits and in the quenched residue were




examined in particular.




     The objectives of the studies were:  (l) to determine the distribution




and survival patterns of pathogenic microorganisms before, during, and




after  incineration; (2) to evaluate the efficacy of the various  incinerator




operations  in destroying pathogens; (3) to aid in the development of design




criteria and operational standards for solid waste incineration.

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     This progress report describes the efforts which have been made




toward the attainment of the research objectives.




     The development and perfection of sampling and analytical methods




were investigated first.  Because of the extreme heterogeneity of ordinary




municipal solid wastes, microbiological sampling and analysis procedures




in present use were thought to be inadequate and inappropriate in most




cases.  Another upsetting factor was the large day-to-day variation




observed in overall waste characteristics; for example, that essentially




paper only was observed on one day and mostly packing house wastes on




another.  The first sample available was too large (a truckload)  to




attempt simple mixing for homogenization.  The sampling problem was




overcome by taking random 100- to 200-g samples from throughout the




available refuse, compositing into one sample (2,000 to 4,000 g)  and,




after thorough mixing, extracting the final 200-g subsample.




     Eight municipal  incinerators were studied and two to six composite




samples obtained over individual 8-hr-run periods.  Each incinerator residue




and quench water sample was obtained after a minimum 2-hr period of




steady operation.  This permitted attainment of standard operating




temperatures within the incinerator.  Selected refuse samples before




incineration and residue samples after incineration were collected with




sterile tongs and placed in sterile 200-ml specimen cups.  Quench water




was collected in 250-ml capped bottles.




     A statistical evaluation was made to determine the size and number




of refuse and residue samples required to isolate an enteric pathogen.




As a result of this investigation three subsamples in duplicate,  each




weighing 30 g, were analyzed from each of five incinerators.21





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     Quench water (1,000 ml) was collected on each sampling day from each

of three incinerators and analyzed for indicator organisms and for the

presence of enteric pathogenic bacteria by concentration on a diatomaceous-

earth layer.

     The presence of microorganisms in the stack effluent of a conical

burner at Memphis, Tennessee, was measured in three locations:  the primary

outlet, the electrostatic precipitator outlet, and the scrubber stack.  The

samples were taken with an impinger method adopted to this type "of

incinerator.13

     A quantitative study was made on the microbial flora of the dust from

six municipal refuse incinerators with particular emphasis on bacteria of

proved pathogen!city for the respiratory and intestinal  tracts and the

skin.  Sampling procedures were carried out with Andersen Sieve Samplers*14

in specific waste handling areas, such as the dumping floor, the charging

floor, and the  residue areas.


                           Materials and Methods
     Sampling Procedures.  The eight incinerators examined in this study

were located in Cincinnati, Ohio, Chicago, Illinois, Memphis, Tennessee,

Atlanta, Georgia, and New Orleans, Louisiana.  The incinerator designs

ranged from relatively old batch-fed units to more modern continuously

fad rotary kiln units.  Each incinerator was operating in a normal manner

at the time the samples were collected.  No attempt was made to collect

data describing the operating characteristics of the incinerators during
     ^Mention of a commercial product does not imply endorsement by the
U.S. Government.

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     The air samples were taken at three areas of the  incinerators:  the




dumping floor area, the charging floor, and the residue areas.  The




dumping and the charging floor areas are consistently  the dustiest areas




of the incinerator.  These are the areas where there is the most open-air




handling of the refuse.  The refuse falls from the collection truck  into




a pit, is lifted by a crane up to the charging floor,  and is dropped into




a chute.  The samples were taken at a height of approximately 5 ft in work




areas.




     Sample Preparation for Analysis.  Preliminary Studies.  Lack of




information on recovery of viable bacteria from refuse material




necessitated preliminary studies.  The Waring Blendor  (Model 1088, Waring




Products, Winsted, Conn.) was used for sample homogenization.  Autoclaved




refuse samples, inoculated with I-ml aliquots of log phase Esaheriehia




ooli cultures (7.8 to 8.5 x 108 cells per ml) were suspended in cold 0.067 M




phosphate buffer,  pH 1.2, and homogenized for varying  periods of time at




free running speeds of 17,000 rpm to determine the optimum for maximal




release of viable cells.  The optimum homogenization time was found to




be 15 sec.16




     Preparation of Field Samples.  All samples of refuse and residue




subjected to quantitative analyses were homogenized as slurries in cold




0.067 M phosphate buffer, pH 7-2, in sterile, stainless-steel, Blendor




vessels.   Blending time for field samples was 15 sec.  After blending,




each homogenate was overlaid with a sterile cheesecloth pad to clarify




the fluid for pipetting.  Ten-fold serial dilutions of the homogenates




were prepared in cold, phosphate-buffered water.  Salmonella and other




enteric pathogens  in quench water samples were concentrated by filtering

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the sampling periods.   Sampling times were selected to allow at least 2 hr




of continuous, normal  operation before testing.   Random 100- to 200-g




samples of refuse before incineration and samples of the residue after




incineration were collected with sterile tongs and placed in sterile 200-ml




specimen cups.  The random samples were composited into 1 sample of 2,000-




to 4,000-g size.  After thorough mixing a final  200-g subsample was




assayed.  Quench water was collected in sterile 250-ml capped bottles.




Quantitative studies enumerated total viable bacterial cells, total




coliforms, fecal coliforms, and heat-resistant spores.  Thirty-gram




subsamples of refuse and residue and 800-ml quench water were assayed




for enteric pathogenic bacteria such as Salmonella and Shigella.




     Stack effluent samples were taken with an impingement method adapted




to this type of incinerator.  The effluent was drawn through a 1/4 in.




sterile, stainless steel tube by a 1.0-cfm vacuum pump.  The tube was




cooled by a water jacket.  The effluent passed through the tube and was




impinged into a 300-ml aliquot of sterile 0.067 M, pH 1.2, phosphate buffer




in a 1,000-ml bottle.15  The sample was run for 10 min, yielding a 10




cu ft sample.




     Air samples were taken with an Andersen sampler.  This  is a six-stage




multijet sampler that separates airborne particles into six aerodynamic




sizes covering the range for respiratory tract penetration.  Each of the




six stages of the sampler covered a  petri dish containing 26 to 28 ml of




solid agar medium.  Air was drawn through the sampler at  1.0 cu ft per




min (cfm) with a vacuum of  15  in. of mercury.  Because of the  larger




number of organisms involved, the sampler was run for 15  sec (0.25 cu




ft air)  in order to get well separated colonies  in the  range of 1 to 30.

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an 800-ml sample through a 1-in. diatomaceous-earth (Johns-Manvi1le,




Celite 505) layer placed on the screen of a stainless-steel membrane




filter holder.  After filtration, the diatomaceous-earth layer was




removed from the holder, divided in half, and analyzed.  Refuse and




residue samples for enteric pathogens were examined directly without




homogenization.




     Quantitative and Qualitative Methods.  Routine quantitative methods




were modified to suit solid waste or waste material characteristics for




the determination of:   (1) total viable bacterial cell number;  (2) total




and fecal coliform organism counts; (3) heat-resistant spore count.




     Total viable cell counts were made by spreading 0.1-ml aliquots of




the serially diluted homogenates or liquids on the surfaces of blood agar




plates containing trypticase soy-agar base (BBL) and 7 percent defibrinated




sheep blood, and by the pour plate method as described in Standard Methods




for the Examination of Water and Wastewater.15  Total  and fecal coliforms




were estimated by the Most Probable Number (MPN) method.15  Lauryl tryptose




broth (Difco) was used  in the presumptive tests at 35 C; brilliant green




bile broth (BBL) at 35 C and EC broth (Difco) at M.5 C were employed in



the confirmed tests.  Further confirmation of the presence of coliforms




was achieved by streaking aliquots of positive tubes on eosin methylene




blue agar (BBL).  The number of heat-resistant spores in the homogenates




or liquids was determined by heating samples diluted in 0.067 M phosphate




buffer, pH 1.2, at 80 C.  Viabiliby at 15 and 30 min was determined by




incorporating 1-ml aliquots of the samples into pour-plates of tryptone




glucose extract agar (Difco).  All plates were counted after 2k- and ^8-hr

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incubation at 35 C.  All populations were calculated as cell  numbers per




g wet-weight raw refuse or quenched residue, per 100-ml quench water, and




per cu ft stack effluent.




     The Membrane Filter Technique was used to determine total and fecal




coliform densities present in raw solid waste, quench water,  and residue




from three incinerators (two in Atlanta, Georgia, and one in  New Orleans,




Louisiana).  At the other five incinerators evaluated, MPN methodology




was used.




     To isolate salmonellae and other enteric pathogenic bacteria 30-g




of refuse or quenched residue were placed in each of two wide-mouth, 500-ml




flasks.  One flask contained 270 ml selenite F enrichment medium (BBL




product) and the other flask contained 270 ml selenite brilliant green




sulfa  (SBG) enrichment medium (Difco product).  The flasks were well




mixed and incubated at 39-5 C for 16 to 18 hr.  On two occasions, 41.5 C




incubation temperature was used.17




     After incubation, dried plates of salmonella-shigella (SS) agar




(Difco), bismuth sulfite (BS) agar (Difco), and McConkey's agar (Difco)




were inoculated by streaking one loopful of the selective enrichment




culture.  Each enrichment was streaked on four plates of each agar.  Thus,




each sample ultimately required a total of 12 plates.  All agars were




freshly prepared except for bismuth sulfite agar, which was used after




3-day  refrigeration.18  The inoculated plates were incubated at 37 C for




2k to 48 hr, whereupon three to six characteristic colonies from each




plate were picked to slants of triple sugar  iron (TSl) agar (Difco).  The




slants were incubated overnight at 37 C.  Further identification, biochemical

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differentiation and serological grouping of Salmonella, was based on the




procedures developed at the Communicable Disease Center, Atlanta, Georgia.19




     Pathogens concentrated on diatomaceous-earth layer from quench water




samples were isolated by the method described above except that each half




of the layer was suspended in 90-tnl amounts of each enrichment medium.




     Analysis of Dust.  For analyses of dust the Andersen sampler was used




with two types of media, trypticase soy agar (ISA) (BBL), containing 5




percent sheep blood and eosin methylene blue agar (EMB) (Difco).  The




TSA/blood agar was used to isolate a wide range of fastidious organisms




such as Diploaooous pneumonias, beta-hemolyt-ie Streptoeooeuss and




Staphyloeooeus aureus.  The EMB agar was used to isolate gram-negative




bacilli.   The plates were incubated aerobically at 37 C for 2k hr




(preliminary studies showed that very few organisms in the dust would




grow under anaerobic conditions).  The enumeration of colonies was made




with a Quebec counter.  Each colony was categorized on the basis of its




macroscopic, microscopic, and physiologic characteristics.  The organisms




were placed under four general categories:  gram-positive cocci, gram-




negative bacilli, gram-positive bacilli, and fungi.  Gram-positive cocci



were further classified as staphylococci, streptococci, and pneumococci.




The count of gram-positive bacilli and fast growing fungi was made from




the TSA/blood agar.






                                Discussion
     Field Survey Sampling Techniques.  One of the initial problems was



the establishment of the methodology for sampling and analyzing raw solid

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waste, and incinerator solid, liquid, and gaseous effluents.  The sampling




problem of solid waste materials was overcome by taking random samples




from throughout the available refuse and incinerated residue and compositing




into one sample (2,000 to ^,000 g) and after thorough mixing a final 200-g




subsample was extracted and analyzed.




     A portable sampler was designed to enumerate viable microbial cells




in incinerator stack emissions.  This sampler provided a method for




qualitative sampling of gaseous stack effluents.




     An Andersen sampler was employed to determine the viable microbial cell




number in incinerator aerosols.




     A statistician was consulted who determined the size and the number




of solid waste and residue samples required so that there would be 95




percent chance that at least one sample would be positive for enteric




pathogenic bacteria.  It was postulated that the number of enteric




pathogen cells present in a given quantity of solid waste or waste  residue




followed a Poisson probability distribution.  From a study (recently submitted




for publication) it was determined that three 30-g subsamples (in duplicate)




are required to yield a pathogen-positive sample.21




     Raw refuse and residue samples were homogenized as slurries  in cold,




0.067 M, phosphate buffer  in sterile, stainless-steel, Blendor vessels.




Preliminary studies showed that the optimum homogenization time was 15  sec.




     Routine quantitative methods were adapted or modified to suit  raw




solid waste or  incinerator waste  related material characteristics for the




determination of:  total viable bacterial cell number, total and  fecal




coliform organism counts, and heat-resistant spore count.  Methods  were

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developed to isolate Salmonella organisms from solid waste, waste residue,




and quench water.



     Results of the studies on microflora associated with municipal solid




waste at eight municipal incinerators showed that the average number of




viable bacterial cells  in the raw refuse samples before incineration




were relatively constant from one incinerator site to another; total




viable bacterial population in raw refuse ranged from 4.0 x 105 to 6.8 x




108 organisms per g; total coliform densities ranged from 3.4 x 105




to 5.1 x 107 organisms  per g (Table 1).  The presence of the coliform group




in large numbers indicated sewage pollution; in lesser amounts it could




denote contamination from other sources since coliform subgroups are




known to occur in animal excreta, soils, grains, grasses, and other




vegetation.  The estimated total number of coliforms discharged daily




by one man is 13 million organisms per g.20




     Fecal coliform population in raw refuse from the eight incinerators




ranged from 1.5 x 104 to 8.1 x 106 organisms per g (Table 1).  The




significance of fecal coliform organisms to waste quality is that the




origin of this organism is from the intestinal tract of warm-blooded




animals, including man.  Consequently, the presence of fecal coliform




bacteria in the waste can be interpreted as an indicator of contamination




of the waste by fecal matter.   The contamination of waste by fecal matter




may be one avenue of transmission of pathogenic microorganisms to the




environment and man.
                                  11

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     Total Incinerator Effluent Microblal Quality Showed Large Variation.




Significant differences were found in the microbial quality of residue from




the eight incinerators tested.  Incinerator I  produced the residue of poorest




quality in terms of total viable cells, heat-resistant spores, total and




fecal coliform organisms (Table 1).  Incinerators VI, VII, and VIM produced




residues containing small numbers of total viable cells, spores, and




coliforms (Table l).  The best residues in terms of fecal coliform densities




were obtained from  Incinerators IV and VIM, fecal coliform densities were




less than 10 organisms per g of residue for both incinerators (Table 1).




     Quench water tested from Incinerators VI  and VII showed coliform




densities of less than 100 organisms per 100 ml.  Total and fecal coliform




counts of 2.9 x 104 and 1.7 x 10^ organisms per 100 ml were found in quench




water from Incinerator VIM  (Table 1).




     Salmonella derby and Salmonella st.  paul were isolated from quench




water and residue obtained from Incinerator I.21  The health significance




of this finding is that if such a rich flora can be found in the burned




residue, pathogens buried with the waste material can survive for an




undetermined time and can be transported out of the fill by way of leaching




water, can reach a waterway, and can become health hazards.  Waterborne




outbreaks of typhoid, paratyphoid fevers, and other intestinal disorders




due to Salmonella organisms  have been well documented.22




     None of the incinerators tested in this study produced a sterile




residue.  The recorded incinerator temperatures (1,200 to 2,000 F) would




eliminate all viable microorganisms if the refuse itself approached such




temperatures during the burning process.   Unburned material such as
                                   13

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vegetables and even newspapers were seen in the residue at some of the




incinerators tested.  Survival of large numbers of microorganisms in the




residue indicated that the temperatures recorded at these municipal




incinerators provided no assurance of a satisfactory operation from the




standpoint of public health.




     The presence of microorganisms in the stack effluent was measured




at Incinerator V (conical burner).  The stack data from this indicated




that a few gram-positive bacilli  (2 organisms/cu ft air) were passing




through the stack,  but that most were scrubbed out.23




     Results of studies made on dust from the waste dumping areas, the




charging floors, and the residue areas at six municipal incinerators




showed that total viable microbial cell counts ranged from 10 to  197




organisms per 0.25 cu ft air  in the waste storage areas; 20 to 160 and 1




to 105 organisms per 0.25 cu ft air in the charging floor and the residue




areas (Figures 1 to 3).  The commonest organisms present in the dust were




fungi, gram-positive cocci, and gram-negative bacilli, most of which are



associated with body wastes and the earth material in the refuse.  In




Incinerator VI (rotary-kiln type), the level of microbial cells was




considerably lower than  in other incinerators.  This difference was




presumably because Incinerator VI was newer and kept cleaner than the




other incinerators sampled.




     Comparative data for airborne microbial populations in various




environments per 1 cu ft air  is shown below211:

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          Envi ronment
 Total  microbial  levels
(colonies  per cu  ft  air)
     Country ai r

     General offices and schools

     City streets

     Factories
         56

         95

         72

        113
The levels of microorganisms in the dumping and the charging floor areas of

the municipal incinerator studied were often 4 to 10 times higher when

compared with the levels in those other environments.  By comparison,

city streets contained approximately 18 viable organisms of all these types

for the same volume of air (0.25 cu ft).

     The incinerator dust was found to carry pathogenic microorganisms,

such as Stophylooocous aureus,  Diplooooous pneumonias, and Ktebsielta

pneimoniae, although these represented a small percentage of the total

number (Table 2).  These organisms are associated with skin and upper

respiratory tract ailments.  Esaheriohia ool-i was found in the dust at

five incinerators tested, indicating the presence of fecal wastes.

     There was a great deal of variability in microbial types found in

dust between one incinerator and others, and even between the dumping and

the charging floor area of one incinerator.  This was expected because of

the fact that the dust is undergoing constant movement and change of

composition.  Although the kinds of specific organisms varied widely  from

one incinerator to another there was a pattern which was common to all

incinerator sites sampled.  The dust from the dumping and charging floor

areas of all the incinerators investigated contained a-hemolytic

                                  18

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streptococcus, which is usually considered to be human-borne, present in



the upper respiratory tract of all  normal individuals.  This organism has



been shown to be a good index for the presence of respiratory tract



pathogens.25  It is an easily recognized organism and survives well In



air.  It seems from the data that respiratory tract pathogens should get



the major consideration in view of the small  number of fecal col I forms



observed.  Experimental studies have shown that the number of small



particles necessary to infect through the alveoli of the lung is very



small, often 10 organisms or less,  whereas the doses necessary to  infect



with larger particles through the upper respiratory tract are uniformly



great, often 1,000 to 10,000 organisms or more.26  Thus, any incinerator



air rating method should probably include a consideration of the total



number of viable organisms and a consideration of the a-hemolytic



streptococcus as an indicator organism.



     It should be noted that this discussion pertains to the threat of



upper respiratory tract conditions and disregards the other threats to



the health of incinerator workers such as dermatitis.



     The data obtained from this study reinforces the already obvious



fact that adequate dust removal equipment is needed to prevent the creation



of  infective aerosols.  If, in the future, epidemiologlc justification



for microbial control measures in air during waste processing becomes



evident, this report might serve as a guideline to feasibility.  The



techniques employed in this study and parameters used might be considered



effective in a monitoring program.
                                  20

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     This report has been compiled to disseminate as widely as possible



the latest information and findings of a research project on microbiological



quality of municipal waste and incinerator residue.



     It is hoped that this report will provide useful information to those



working in the solid waste management field and assist in developing



approaches to the solutions of their solid waste disposal problems associated



with microbiological hazards.





                             Project Summary





     Objectives.  There were three primary objectives of this study:   (1)



to determine the distribution and survival patterns of pathogenic microorganisms



before, during, and after incineration; (2) to evaluate the efficacy of



various functional  incineration operations employed in destroying pathogens;



(3) to aid in the development of design criteria and operational standards



for solid waste incineration.



     Approach. Microbiological methods were developed to measure the



levels of microbial pathogens associated with municipal waste.  The



efficacy of several incinerator types to destroy bacteria associated with



solid waste was evaluated, with emphasis on fecal and food sources.  Solid



waste samples and incinerator residue taken from eight municipal



incinerators of different design were examined for total bacterial cell



number, total coliforms, fecal conforms, heat-resistant spores, and



selected enteric pathogens.



     Results.  Of the eight incinerators tested, only one produced residue



devoid of fecal coliforms; the seven others produced residue containing
                                  21

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fecal coliform population of less than 1.0 x 101 to 4.7 x 103 per g.



Salmonella organisms were found in quench water and residue from one of



the eight incinerators tested.



     Stack analysis showed that a few viable bacterial cells (2 gram-



positive bacilli) were passing through the stack of a conical burner.



     Dust samples taken from six municipal incinerators at three specific



waste handling areas showed total microbial cell counts of 1 to 197



organisms per 0.25 cu ft air.  Organisms associated with upper respiratory



and intestinal tracts were found.






                            Ack now1edgmen t s






     This investigation was initiated and completed by the former Public



Health Effects Projects Branch under the Division of Research and



Development, Solid Waste Management Office.



     We are appreciative of the encouragement, guidance, and assistance



given by personnel from the Division of Research and Development and



Technical Operations during this study.  Appreciation and thanks are



especially extended to Dr. Fred J. Stutzenberger, Messrs. David Armstrong,



Donald F. Spino, and Henry Johnson,  and Mrs. Janet C. Blannon for their



technical assistance and consultation.  We gratefully acknowledge the



valuable criticism and assistance of Mr. Louis W. Lefke In the



preparation of this report.
                                  22

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                                References
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