GREAT LAKES-ILLINOIS  RIVER BASINS
        COMPREHENSIVE  STUDY
     INTERIM REPORT-ILLINOIS RIVER BASIN
     Appendix V-Biological  Investigations of
               the Upper  Illinois Waterway
                August  1961
 U. S. DEPARTMENT OF HEALTH, EDUCATION, AND WELFARE
             Public Health Service

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                         INTERIM   REPORT

                   GREAT LAKES-ILLINOIS  RIVER BASINS
                          COMPREHENSIVE STUDY
                1.  INTERIM REPORT - ILLINOIS RIVER BASIN
                      Appendix V - Biological Investigations of the
                                Upper Illinois Waterway
                                AUGUST, 1961
PUBLIC HEALTH SERVICE
      REGION V
   CHICAGO, ILLINOIS
GREAT LAKES-ILLINOIS RIVER
      BASINS PROJECT
     CHICAGO, ILLINOIS
                         FIELD OPERATIONS SECTION
                 ROBERT A. TAFT SANITARY ENGINEERING CENTER
                              CINCINNATI, OHIO
               DIVISION OF WATER SUPPLY AND POLLUTION CONTROL
                           PUBLIC HEALTH SERVICE

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                                         SUMMARY
   The  analyses of data that were collected
during  the  biological survey  indicated  that
definite degradation by man-contributed pollu-
tion  had defiled the canalized waterway in the
reaches  studied; bottom animals  especially
are expressive of degradation.

   The April  -  May 1961 biological sampling
in the Upper  Illinois Waterway of the Metro-
politan Chicago Area revealed associations of
bottom animals indicative of highly degraded
water. The bottom  animals show responses to
both domestic sewage and wastes of industrial
origin as degradation agents.

  The water  quality  and bottom  conditions
determine the type and quantity of organisms
present.  As long as the water and  substrate
remain degraded by organics of domestic origin
the presence  of organisms commonly associ-
ated with pollution is desirable. These organic-
waste-tolerant  bottom   animals,   (notably
sludgeworms) are important natural agents in
"self-purification."  If  these organisms are
hindered  in  their  activities by  materials  of
industrial origin or by too severe conditions
related to domestic sewage pollution the rate of
"self-purification" is slowed. A slower rate of
"self-purification" in a river results in a down-
stream extension of the area  in which water
remains degraded. In essence, biota associated
with  organic degradants,  cannot  contribute
their share to the  natural "self-purification"
of a waterway if inhibited by  an unfavorable
environment

   Phytoplankton aid  the  "self-purification"
process  in  a stream.  Populations of phyto-
plankton  contribute  to  the oxygenation of a
stream through  photosynthesis. If phytoplank-
ton populations  are  hindered in  their growth
and  function,  the  efficiency  of a stream's
"self-purification" process may be  reduced.,

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                                     INTRODUCTION
   The  bulk  of  the  biological data  reported
here  are  based  on  the  sampling of bottom
organisms and phytoplankton  between April
19 and  May 11, 1961. Some general informa-
tion  on  fish,  that were gathered from casual
observations, are included.

   Bottom  organism samples were collected
with the Ekman dredge (Fig. IB) with some
supplemental exploratory  sampling done with
the Petersen dredge (Fig. 1A). Phytoplankton
samples were  analyzed from unconcentrated
water  samples  that  were   taken from  the
bacteriological sample  bottles. For  an  im-
mediate  visual  determination of the gross
bulk of phytoplankton  that were  present  ten
liters  were  concentrated through a  standard
(Number 12) plankton net.  (Fig. 2).

   The  bottom organism  biological  data es-
pecially indicate  that all areas of the Illinois
Waterway that were studied in Cook County
were degraded. Even  the stations  with  the
greatest variety of types of bottom organisms
collected in Cook County  show indications of
pollution (i.e., immediately below the Wilmette
lockage, Mile NS340.7, and immediately below
the Chicago Harbor lockages, Miles CH326.9
and CH325.8). Cleaner water associations of
bottom  organisms were represented only on
the Des Plaines, Mile DP292.7, andtheKanka-
kee Rivers, Mile KR277.5. (Figs. 3 and 4).

   Dredge sampling indicated that the bottom
life typically consists of organisms  that are
associated with organic domestic-sewage type
sludges. The sludges from which bottom or-
ganisms were taken commonly revealed gar-
bage particulate matter and sewage in various
stages of decomposition accompanied by fecal
odors, often masked with petroleum odors and
often mixed with oily materials.  (Fig. 5)

   The dominant bottom organisms at all sta-
tions  sampled, in Cook County, were sludge
worms, that reached tremendous populationso
(Fig.  6). These  same  worms are often  as-
sociated  with  sludge-drying beds at sewage
treatment plants,  and are  obvious  in small
creeks throughout  the country where sludge
from  domestic sewage pollution accumulates
on stream  bottoms. In shallow streams which
afford visual advantages of the observation of
the bottom,  not afforded  in the canalized
reaches  of  the  Illinois  Waterway that were
sampled, populations  of them can be seen as
red patches living  in decomposing domestic-
sewage sludges.  Here  they  burrow into the
upper surfaces of sewage sludge as they feed,
leaving their  rear ends extending  into  the
water. Such red-colored, wormy blankets form
a signal  to the investigator that the stream is
being defiled with organic wastes that may be
issuing from  a domestic sewage  source,  or
sources,  or from an industrial outfall rich in
decomposable,  putrefactive  organics as  dis-
charged from a slaughter house,  cannery, beet
sugar  refinery,  etc.  On  occasion these  red
sludge worms are found in  natural lakes and
streams  that  are not subject to man-made
pollution; here they do well on natural organics
such as may be formed from dead and decaying
aquatic weed and algal mats  interlaced  with
leaves that have been washed into the water, or
they may be found blanketing the putrefying
carcass  of  a  dead beaver,  muskrat, fish  or
water fowl.

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Introduction
                      V  ":x
                       '"-','  , . .*i-

                        %*  -  '..
         FIGURE 2.  Plankton Net Utilized For Gross
                      Visual   Bulk  Determinations  of
                      Phytoplankton Quantity.

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Introduction

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   BIOLOGICAL INVESTIGATIONS OF THE UPPER ILLINOIS WATERWAY
CENTIMETERS
          FIGURE 5.  A Photograph Showing the Diversity and Quantity
                     (per 1/4 square foot of bottom surface) of Vari-
                     ous Kinds of Organisms at Station CH326.9.   A
                     Similar Sample Taken From Station  SS314. 0   is
                     Shown in Figure 6.

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                                  Introduction
CENTIMETERS
          FIGURE 6.  A Photograph Showing the Quantity of Sludgeworms,
                     the Only Organism Present, in 1/4  Square Foot of
                     Bottom Surface at Station  SS314. 0.  Compare This
                     to  the Organism Diversity and Quantity That Occurs
                     at  Station CH326. 9 Illustrated in Figure 5.

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                                  BOTTOM ORGANISMS
   When discussing the response of benthic
organisms,  other  than bacteria, to pollutional
environments several aspects must be con-
sidered. Some of  these are  discussed  in the
body of the text.* It would be wise  to briefly
review these principles and elaborate on them.

   Many times biologists speak of "pollution
indicator"   benthic  macroorganisms.   This
phraseology  is  erroneous to the uninitiated.
There  are  no macroorganisms that indicate
pollution by their presence. The benthic macro-
organisms  referred to as "pollution indicators"
also exist  in the natural environment. The
"pollution indicators" have existed long before
man began to dump his wastes into any environ-
ment. They have not recently evolved to occupy
new environmental niches. These organisms
are the  species that are adjustedphysiological-
ly and  morphologically  to survive  environ-
mental  conditions  that are adverse  for most
benthic  macroinvertebrates.  Their  less ad-
justed relatives are driven from the area or
killed by the adverse  conditions imposed  on
them.

   When only a few varieties survive in an area
they reproduce  and their young  survive  in
enormous abundance. With  the removal of less
tolerant forms,  competition  between kinds is
reduced, thus allowing the remaining forms to
increase until they  compete between  them-
selves.  The level at which individual kinds begin
to compete among themselves  varies from
situation to situation. At some point in their
growth some factors apparently create so much
competition that further increase in the popula-
tion is not possible. If food, oxygen, and other
environmental conditions are not limiting even-
tually the population stops growth because there
is no more space, physically, to put additional
organisms. Populations would reach this level
in exceedingly rare cases.  Usually such things
as food, temperature, toxicity, currents, light,
and other  factors will limit the populations
before the  area is physically saturated.  In
many  situations  it  is  food availability  that
controls  the level at which competition limits
the size of the population.

   In areas of heavy organic (food) deposition,
the associated physical and chemical factors,
drive competitive forms from the  area.  The
quantity  of  food material  being  deposited
limits the growth of the few remaining forms0
Thus,  as  organics are added competitive
forms are driven from the area, and surviving
forms increase  in  numbers  due to  both the
removal  of competition and/or increased food
supply. If enough organics are added, eventu-
ally a "break point" occurs when organic de-
composition  products  create  an environment
so  severe that few, if any  organisms,  can
survive.

   Another type of pollutant  is the non-organic
waste. In  this situation various  forms  are
eliminated.   The  forms surviving  increase
slightly or not at all. Their  maximum number
is  rapidly  reached  because  nutrition  levels
do  not change. Competition is removed from
other forms, but the  population increase  is
soon checked by  competition for food between
individuals of a form.

   As these non-organic wastes increase they
will eventually reduce the few surviving forms
in  number  by interfering with reproduction,
killing individuals periodically before popula-
tions  can become well  established, destroying
the food source, or by making food unavailable.
If  enough waste  is added,  eventually condi-
tions  become  so  severe that even  the  few
remaining tolerant forms are eliminated from
the environment.

   In  complex degradation  systems  of both
organic and inorganic  wastes the  two effects
work together.  Initially they reduce the number
of  types.  The  remaining few types are then
* Refer to "Interim Report Lake Michigan-Illinois River Basins Comprehensive Study,"pp. 67-69,

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                                       Bottom Organisms
affected by an interaction of the two pollutants.
The non-organic may limit the population even
though  large  quantities  of  organic food are
being added. Large quantities of organics may
erase the influence of the inorganics by dilution
and adsorption. With different  types of inor-
ganics entering the environment, it is possible
for the  effects  of one to be reduced by chemical
action of  another  on it; or it is possible for
two inorganics to function more severely when
together than if kept separate. Thus, in com-
plexes of  many types of pollutants being added
to an environment there  is a constant interplay
of  influences  on  the environment at various
points.

   In discussion of the degradational influences
on   the macrobenthic fauna of the Chicago
Waterways many things must be  considered.
The ideal situation  of one sewage outfall with
one type  of waste  is  eliminated.  The total
number of outfalls may never be tabulated and
located,*  when one considers the addition and
elimination  of sewers in a metropolitan area as
large and diversified as Chicago.  Before one
waste effluent is mixed with the water another
effluent is  dropping into the Waterway. For
miles,  (in  under bridges,  from  industries,
shipping,  runoff, etc.),  there are waste addi-
tions to the  system varying  in size from small
tube  drippings to  the  watershed  itself.  In
addition there is not one type of pollutant, there
is  a complex, from  human  excrement  to
garbage,  petroleum  wastes,  street  runoff,
heavy  metallic  ions,  tannery  wastes,  trash
dumping, sewage outfalls, etc.; a list of varie-
ties that may never be compiled in  a constant-
ly  changing  metropolitan industrial complex
of the magnitude of Chicago.

   The  simplest way of analyzing the system
is to visualize  it as a large river system with
the headwaters at  Lake Michigan consisting of
the North Shore Channel below the Wilmette
lockage, the Chicago River below the Chicago
Harbor  lockage, and the Calumet - Sag Canal,
all flowing into the Illinois River. This concept
does not  account for  various geographical
entities and historical names; but when one
considers the man-made alterations, impor-
tance,  direction and volume of flow, this arti-
ficial stream  complex is the major drainage
pattern  of the Greater Chicago Area. In essence
there are three major headwater streams in the
metropolitan area which eventually, upon junc-
tion with the Des Plaines and Kankakee Rivers,
form  the Illinois River.

   A glance at the data indicates one sampling
station  distinctly different  from all the other
samples.  (Figs. 3 and 4). This is the Kankakee
River; the only  Station (KR277.5) that exhibits
organisms  not  tolerant of  degradation. The
quantitative samples exhibit seven major forms
of benthic animals, two of  which, Caddisflies
and Mayflies, tolerate very little environmental
degradation.  In  addition to  the quantitative
data,  two  additional forms, Scuds and Damsel-
flies,  which are sensitive to degradation are
known to  exist. Those forms present that will
tolerate pollution are relatively low in quantity
and do not contribute to an  abnormal standing
crop dominated by one or a  few fortnSo

   The other  stations all had one phenomenon
in  common.  There  was a general lack  of
organisms that will tolerate adverse conditions
resulting  from pollution. Beyond this, the sta-
tions  varied but little in the kinds of tolerant
forms present, but varied tremendously in the
quantity of  the kinds present.  (Figs. 3  and 4)0
   Beginning  at  the  upstream point,  in  our
general flow pattern, just below the Wilmette
lockage there were four kinds present, (Fig07)0
One of these, the Scud, is not typically tolerant
of organic  pollution. The  small numbers  of
Scuds present make it doubtful if they reproduce
in this  area; the  standing crop may  be re-
plenished by  individuals carried through the
lockage from Lake Michigan. During the period
of investigation, April  19  to May 9,  1961,
diversion of lake water was obvious. Even with
the heavy flows there was evidence of organic
sludges which may build up to a considerable
extent during periods of nondiversion.

   From the Wilmette lockage downstream via
the North Shore Channel and the North Branch
of the Chicago River a typical organic degrada-
tion occurs. (Fig.  7). The  reduction in types
of kinds,  from  four  at the  Wilmette lockage
(NS340.7)   to  one  nine miles downstream
(NB331.4), and  the increase  from 1,300  to
47,700 Sludgeworms  per square foot at these
respective stations is indicative of the organic
sludges being deposited in  the waterway sys-
tem.
* A tabulation of known sources is listed in the "Interim Report Lake Michigan-Illinois River Basins
 Comprehensive Study," pp. 70-118.

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10
BIOLOGICAL INVESTIGATIONS OF THE UPPER ILLINOIS WATERWAY
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12
BIOLOGICAL INVESTIGATIONS OF THE UPPER ILLINOIS WATERWAY
deposition.  Considering  the  lack  of sludge
deposition  there  remains a fair quantity of
Sludgeworms that subsist on water suspended
organics such  as human feces; garbage: as
peas, corn, celery, and lettuce, etc.

   Impounded areas behind the navigation pools
(DP285.8 and IR271.5), the Brandon and Dres-
den  Pools  respectively,  with  their  standing
water, may be considered as settling basins.
The  sludge deposits in these impoundments
occupy  a  large  portion  of the reservoirs.
Water depths of only a few feet, outside of the
navigation  channel,   may  be  found  a short
distance behind  the thirty-eight  foot  high
Brandon Road  Dam.  In these areas  a build-
up in  the  quantity  of  Sludgeworms  occurs.
(Fig.  3).  Bloodworms and  Fingernail Clams
were also found in the Dresden Pool.
   In the region below the Lockport  Locks
the Des  Plaines River joins the complex. The
one  station  sampled on the Des Plaines im-
mediately above the junction with the Chicago
Sanitary and  Ship  Canal,  indicated  a lower
level of pollution  than  that which exists in
the Sanitary and Ship Canal. Direct comparison
with  the Chicago  Sanitary and Ship  Canal
Stations  is not feasible. The Des Plaines Sta-
tion  (DP292.7)  is  physically  very different
from the  remainder  of  the  system,  being
located  in shallow  water  enabling  one  to see
the bottom.  It is also devoid of barge traffic.

   The Calumet-Sag Canal tributary appears
to  be the  most  complex  tributary  in the
system.  The Blue Island  Locks, immediately
downstream from  the Little Calumet  River,
divide this system  into two segments.  From
these locks on  the Calumet - Sag Canal to
the Chicago Sanitary and Ship  Canal, flow is
toward the Chicago Sanitary and Ship  CanaL
This  flow is low in both volume and velocity.

   In the  upper reaches  of this  section  a
confirmed  canalized area (Station CS317.9)
exhibited no organic  sludge and few Sludge-
worms.  This  narrow, confined channel  ex-
hibits strong  vertical  currents from  barge
traffic.   These  currents   apparently  do not
allow settling  of particulate material  since
no  sludge  was  taken  in dredge sampling.  It
may  be assumed  that inorganic  pollutants
and/or benthos disturbance (caused by barge
traffic)  in  this  canalized  section reduce the
expected variety of  organisms.
                                        Downstream  from  the  confined canalized
                                     area the canal widens. Turbulence reduction
                                     allows a settling of organic material (Station
                                     CS308.4) and the standing crop of Sludgeworms
                                     shows  a marked increase, Figure 3.

                                        Above  the Blue  Island  Locks  a "settling
                                     basin" exists. These  Stations  (LC322.4 and
                                     LC320.1) exhibit heavy sludge deposits. This
                                     "settling basin" also extends an undetermined
                                     distance up the Little Calumet (Station LC320.2)
                                     tributary.

                                        Station (LC322.4) shows the greatest organ-
                                     ism diversity in the Calumet-Sag Sytem, Figure
                                     3. Yet  this  station exhibits a possible influence
                                     of partial inorganic pollutants. It is suspected
                                     that periodic  laminar flows of inorganics into
                                     this area may influence the organisms in some
                                     "cross-sections"  of this stream  and not  in
                                     others.

                                        Above the  Blue Island Locks (Station
                                     LC320.1)  to Lake Michigan (Station CA333),
                                     flow is not consistent.  Gradient in either di-
                                     rection is  negligible. Flow is influenced pri-
                                     marily by  the water level in Lake Michigan.
                                     Wind influence on lake levels as well as wind
                                     originated  seiches undoubtedly create inter-
                                     mittent and oscillatory flow direction changes
                                     in this "estuary type" situation.  Rainfall would
                                     also affect flow direction. These influences on
                                     the distribution of organisms cannot be ignored.
                                     With a "sloshing" action, water  degradation
                                     agents are  semi-confined to a segment of the
                                     system. Because of this, water masses may
                                     traverse this area in  two directions for per-
                                     iods of time, before fractions of the water are
                                     discharged in either direction. Dilution is also
                                     slow.   Thus, the organics from the Calumet
                                     Sewage Treatment plant are basically confined
                                     to an area  above  and  below the effluent dis-
                                     charges at  certain times.  The slow progres-
                                     sion of water from  this area allows  organic
                                     materials to settle and break down before the
                                     water moves out of the area. Sludge movement
                                     out of this area is slow.

                                        Another area of "estuary type" is the Calu-
                                     met Harbor (Stations CA333+ and CA328.1).
                                     This area is probably primarily influenced by
                                     inorganics   from  the  heavily  industrialized
                                     environs.  Only  the  Sludgeworm  survives  in
                                     minimal quantities. (Figs. 3 and 4).

                                        A tributary to the system, the Grand Calu-
                                     met River  (Station GC325.8) is unique. This

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                                      Bottom Organisms
river has a low flow and a heavy organic load.       very few Sludgeworms  (40 per square foot of
Sludge  deposits  are  extensive. The sludge       bottom) to  survive. Extended periods of sep-
decomposition  products and associated sep-       ticity prohibit the establishment of quantities
ticity have influenced this organic environment       of Sludgeworms to utilize the organics present.
to proceed beyond the  "break point,"  allowing

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                PHYSICAL  DESCRIPTION  OF BIOLOGICAL SAMPLING
              STATIONS  AND THEIR KINDS OF  BOTTOM ORGANISMS
Station (NS340.7)

   Four types of organisms (i.e., Sludgeworms,
Leeches, Bloodworms, and Scuds) were col-
lected. The former three may be classified as
pollution tolerant forms; whereas the fourth
type,  represented  by three individuals that
were  collected,  may have been transported
from the lake through the lockage.

   The bottom material was a heterogeneous
mixture of inorganics and organics. This area
is canalized  at  this  point,  the  canalization
continuing downstream approximately 2.5
miles below the  Northside Sewage Treatment
Plant.

Station (NS334.9)

   Three types  of organisms  (Sludgeworms,
Leeches,  and Pulmonate  Snails)  were  col-
lected.  This  is  the  first station below the
Northside Sewage Treatment Plant. The stream
is canalized.  The increase in Sludgeworms is
quite evident  even though the substrate is not
complet aly covered by sludge. The bottom is
largely  composed of  hard blue clay.  The
Sludgeworms that were taken were dredged up
as solid balls of worms approximately 1-1/2
to 2"  in diameter.  There was an abundance of
suspended  solids present such as vegetable
fragments.

Station (NB331.4)

   Sludgeworms were the only organism found.
The channel at this station is much wider than
upstream stations and stream velocities were
reduced. Sludge deposition is quite evident. The
number and volume of Sludgeworms  is much
increased   over  the  two  upstream  Stations
(NS340.7 and NS334.9). (Fig. 7).

Station (SB324.3)

   Two types of  organisms (Sludgeworms and
Fingernail  Clams) were collected. Fiberous
sludge was present mixed with silt, sand, and
fragments of Fingernail Clam and Snail shells.


Station (SS320.0)

   Only Sludgeworms were collected. Petrole-
um wastes in bottom materials were apparent.

Station (SS317.3)

   Only  Sludgeworms  were  collected.  The
bottom  was  composed  of blue clay,  some
sand, oil, trash, paper towels, spaghetti, and
fresh-appearing vegetable  matter.


Station (SS314.0)

   Only Sludgeworms were collected. A portion
of the bottom was a jelly-like mass of sludge,
and some of the substrate was formed from
blue  clay.


Station (SS307.9)

   Sludgeworms were the  only organism col-
lected. The bottom was composed  of sludge,
a few rocks, oil, and grease.

Station (SS300.5)

   The bottom  consisted of bedrock. Dredge
hauls contained fist-size rock fragments cov-
ered by the "sewage bacterium," SphaerotiluSo
A few Sludgeworms were  collected from the
rocks  and slime.  Substrate  characteristics
prohibited quantitative sampling.


Station (SS291.1)

   Sludgeworms were the only form collected.
Some organic sludge was  present in addition
to stones, sticks, and tar balls. Barge traffic
apparently prevented sludge deposition.
                                             14

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                        Physical Description of Biological Sampling Stations
                                         15
 Station (DP285.8)

   Only  Sludgeworms  were  collected.  This
 station was  in  the Brandon  pool. This pool
 serves as a settling basin in the waterway
 system. Away  from  the  navigation channel
 sludge  beds  exist just  two  feet  below the
 water surface. Sludges are mixed with grease,
 oil, and balls of tar.


 Station (IR271.5)

   Three  pollution tolerant  forms  (Sludge-
 worms,  Bloodworms  and Fingernail Clams)
 were collected.  The station, located in Dresden
 pool, also serves as a settling basin.  The sub-
 strate is  composed of sludge, sand,  silt, and
 plant detritus. Petroleum odors were notice-
 able in the substrate. Even though this station
 is below theKankakee River junction, organism
 influence from this tributary was not observed.


 Station (CA333+)

   Only a  few  Sludgeworms  were collected.
 This  was an industrialized area. The bottom
 was  made up  of  inorganic  material with a
 trace of organic matter.

 Station (CAS28.1)

   A few Sludgeworms were collected. This is
 an industrialized area. The bottom is composed
 of blue clay, some gravel,  red silt, and oil.

 Station (LC322.4)

   Four  organisms   (Sludgeworms,  Blood-
 worms, Fingernail Clams and Leeches) were
 collected.  The bottom was composed of fine
 loose clay and organic sludge.

 Station (LC320.1)

   Numerous Sludgeworms and Leeches were
 collected. This  station is between the outfalls
 of the Calumet Plant. Industrial outfalls were
 also  observed. The bottom was composed of
 loose clay and silt-like sludge.

 Station (CS317.9)

   Only Sludgeworms  were collected. Barge
traffic apparently prevented sludge deposition.
 Bottom deposits consisted of pea gravel, slate
fragments, and fragments of concrete.
Station CS308.4)

   Sludgeworms only were collected. This sta-
tion is below the  canalized area  of the Blue
Island Lockage. Organic sludge deposition was
quite evident.

Station (GC325.8)

   Only  Sludgeworms  were  collected. The
sludge was heavy, partially decomposed, black,
and  septic.  Sludge was observed rising to the
surface in chunks. Gas bubbles were numerous
and large.

Station (LC320.2)

   Two forms of organisms (Sludgeworms and
Bloodworms) were collected. Organic silt-like
sludge was  present. No barge traffic was ob-
served,  although pleasure craft did use this
area for  dockage. The area is basically part of
a settling basin for the  wastes from the Little
Calumet  tributary  and also for outfall mater-
ials  from the Calumet Plant.

Station (CH326.9)

   Five   types  of  organisms (Sludgeworms,
Fingernail Clams,  Pulmonate Snails, Opercu-
late  Snails,  and Leeches) were collected. The
bottom was composed of a variety  of inorganic
and some organic materials.

Station (CH325.8)

   Five  types  of  organisms (Sludgeworms,
Fingernail Clams,  Pulmonate Snails, Opercu-
late  Snails,  and Leeches)  were  founds Trash,
consisting of newspaper material, cigarette
filters, cellophane, etc.,  was observed in the
substrate.

Station (DP292.7)

   Four  forms  of  organisms (Sludgeworms,
Fingernail  Clams,  Pulmonate  Snails, and
Leeches) were collected. This station  is not
comparable  to other stations,  since the maxi-
mum depth was 18 inches.

Station (KR277.5)

   Seven  forms of organisms (Sludgeworms,
Bloodworms,  Fingernail Clams,  Operculate
Snails, Leeches, Mayflies, Caddisflies) were
collected. This was the most diversified popu-
lation found in the entire system. Some of the

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16          BIOLOGICAL INVESTIGATIONS OF THE UPPER ILLINOIS WATERWAY
forms, such as Mayflies and Caddisflies, are          This area  is used  quite  extensively for
considered to  be clean water  forms. The       boating, swimming,  sport  fishing and water
bottom is composed of sands, silts and gravels       fowl hunting.
with some natural organic material.

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                                    PHYTOPLANKTON
   Data collected during a six-hour period on
both May 10 and  May 11, 1961 illustrate the
total numbers  of phytoplankton per milliliter.
(Figs.  8 and 9). These data supplement similar
data already reported for April 28 and May 4,
1961.* An additional graph (Fig.  10), plotting
the mean  of  total  phytoplankton counts  for
April 28, May 4, May 10, and May 11, 1961, is
presented to summarize the data.

   Data indicate that most kinds were found at
each  of  the  stations  throughout  the Illinois
Waterway  system in  Cook  County, although
numbers of kinds showed considerable varia-
tion from station to  station.

   The data discussed above, based on collec-
tions that were made from all stations within a
six-hour period, indicate that there is a down-
ward  trend in total numbers of algae from
the Lake  Michigan  intakes  into the canalized
system.  Phytoplankton present a transitory
picture at any point in a flowing water system
in that they are  weakly swimming or floating
plants  without powers of locomotion. Thus,
being at the  mercy of natural stream veloci-
ties, they are not  a good  index of a fixed
station.  Too, phytoplankton collections  made
on different days at the same station may vary
widely in numbers per milliliter, since their
abundance at any one station might be dependent
on  the  amount  of floating solids, turbidity,
cloud cover,  and varying  waste  discharges
present at different times of collection. For
example,  these  variations  are demonstrated
through data collected from Stations (SS317.3,
SS307.9,  CA328.1,    and LC322.4)  on days
between April 25, 1961 and May 11,  1961 as
shown in Figure  11. The total phytoplankton
counts per milliliter at Station (SS317.3) ranged
from a low 1,760 to a high of 3,629. At Station
(SS307.9)  the range in total  count was from
1,378 per  milliliter  to 2,576 per milliliter,
while a variation of 652 per milliliter to 3,119
per  milliliter was noted at Station (CA328.1).
At  Station (LC322.4) the variation was from
254  per  milliliter to 2,705 per millilitero

   All phytoplankton counts were made in ac-
cordance with the strip count method as out-
lined in  the Eleventh  Edition  of Standard
Methods for  the  Examination  of  Water and
Wastewater. **
* See Figs. 11 and 12 in "Interim Report, Great Lakes-Illinois  River Basins Comprehensive Study."


** Anon, "Standard Methods for the Examination of Water and Wastewater, Eleventh Edition,"Amer.
  Pub. Health Assoc., New York (1960).
                                               17

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18
BIOLOGICAL INVESTIGATIONS OF THE  UPPER ILLINOIS WATERWAY
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20
    BIOLOGICAL INVESTIGATIONS OF THE UPPER ILLINOIS WATERWAY
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                          Phytoplankton
                                                                           21
                           STATION = SS 317.3


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       FIGURE 11. Variations at Single Stations in Total Numbers
                  of Phytoplankton  Per Milliliter on  Different
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           GENERAL INFORMATION ON FISH IN  THE CHICAGO AREA
   During this survey no intensified sampling
for fish was undertaken. However, limited col-
lecting, visual observations, and conversations
with  people in the various reaches  of this
river system indicate that in some sections of
the system certain types of fish are known.

   Directly  below  the  Wilmette  lockage
(NS340.7)  on April 21,  1961, Bluntnose and
Fathead minnows  were  collected with a dip
net. Further downstream at the junction of the
North  Branch of the Chicago River and the
North  Shore Channel,  (near Station NS333.4)
children  were observed  fishing. No fish had
been caught; questioning revealed that Goldfish
and Carp had been taken at this location.
   On April 27, 1961 within the Chicago Sani-
tary and Ship Canal between Stations (SS320.0)
and (SS307.9) many  recently dead fish were
observed,  consisting of Alewives, Smelts and
Chubs. No live fish were observed at any time
in this area during the survey.

   Dead Alewives were observed in and below
Calumet  Harbor  (CA333+) on  May 9, 196L
Since  the  flow of water there is so haphazard
it  is  not known  if death occurred within the
lake or the harbor.

   On May 4, 1961 live  Goldfish and Carp were
observed in the Dresden Pool, (IR27L5) while
collecting bottom fauna.
                                             22

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