United States
                                 Environmental Protection
                                 Agency
                                 Robert S Kerr Environmental Resg
                                 Laboratory
                                 Ada OK 74820
vvEPA
                                 Research and Development
                                 EPA-600/S2-81-093  July 1981
Project  Summary
                                 Overland  Flow  Treatment  of
                                 Poultry  Processing
                                 Wastewater  in  Cold  Climates
                                 Lloyd H  Ketchum, Jr., Jack L Witherow, Arthur J Cunningham, and Robert
                                 L  Irvine
                                  Land treatment of wastewater using
                                overland flow methods has been
                                effectively used in warm climates. A
                                full scale wastewater treatment facil-
                                ity was evaluated, including overland
                                flow   in northern Indiana,  a cold
                                climate. The  study  emphasized  the
                                evaluation  of the  overland  flow
                                system,  but  the  treatment  system
                                included mechanical  pretreatment
                                facilities, a  storage lagoon, a lagoon
                                for batch chemical treatment of the
                                overland flow effluent and rapid infil-
                                tration for effluent disposal. The facil-
                                ity treated  wastewater from  a duck
                                processing  plant. That wastewater
                                was as amenable to biological treat-
                                ment  as domestic sewage and had
                                similar concentrations of nutrients
                                and solids.
                                  Overland flow systems typically are
                                limited to low permeability soils. This
                                system was located on a sandy loam
                                soil  with very  high  permeability.
                                Bentonite was used to provide a
                                percolation  barrier below the grass
                                covered surface.
                                  The  Project Report describes the
                                design and construction of the facil-
                                ities,  presents and  evaluates
                                monitoring data from 1-V2 years of
                                operation and gives  construction
                                costs.  The many  problems encoun-
                                tered including those associated with
                                cold  weather operation are also
                                described to prevent their repetition.
                                  This Project Summary was develop-
                                ed by EPA's Robert S. Kerr Environ-
                                mental  Research Laboratory. Ada,
                                OK, to announce key findings of the
                                research project that is fully docu-
                                mented in a separate report of  the
                                same title (see Project Report ordering
                                information at back).
                                Introduction
                                  This project demonstrated the use of
                                an overland  flow system in  a  cold
                                climate The system was constructed at
                                the Culver duck processing plant, and
                                was located near the Indiana-Michigan
                                border The major objective of the study
                                was to demonstrate the operation of an
                                inexpensive, simply  operated,

                                inexpensive, simply operated, slaugh-
                                terhouse wastewater treatment facility,
                                which would  meet the 1983 National
                                discharge limitations  A lagoon which
                                collected the overland flow effluent was
                                added to evaluate the effectiveness of
                                batch chemical treatmentfor phosphate
                                and suspended solids reduction and dis-
                                infection A bentonite seal was used to
                                allow overland flow treatment at a site
                                with highly permeable soils


                                Treatment Plant
                                  The wastewater treatment tram con-
                                sisted of five types of treatment units

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connected  for  series  operation  as
shown in Figure  1  The pretreatment
facilities  included a fine screen, Hydro-
cyclonic  Rotostramer,  and  a  gravity
grease separator  Inadequate operation
of those facilities, which were located in
the processing plant, and overloadmgs
resulting from surges of flow,  caused
frequent  grease  and solids discharges
into subsequent treatment units These
discharges clogged  pumps, pipes and
orifices,  causing  pump damage  and
substantial operating problems

  Processing Plant
     Effluent
   Pretreatment
Pump Manhole


Lagoon
  Overland Flow
    Treatment
Chemical
Treatment
Lagoon


Rapid
Infiltration

 Figure  1.    Wastewater   treatment
             plant schematic

  The  pretreated  effluent  flowed by
 gravity into a manhole where two sub-
 mersible pumps were  located  When
 the pumps were off, the flow continued
 by  gravity  into a large lagoon  The
 lagoon  primarily  served as  off-line
 storage during  periods when the over-
 land  flow  treatment plots  were  not
 operating. During lagoon storage, how-
 ever, suspended  solids settled,  bio-
 chemical oxygen demand was reduced,
 and  the  wastewater   characteristics
 changed from  those of a raw waste-
 water to those  of a lagoon effluent, in-
 cluding  high  algae  concentrations
 during the summer
  When the pumps were on and waste-
 water was also flowing from the pro-
 cessing plant,  the pretreated  effluent
 was  pumped directly to the overland
 flow plot During periods when the pro-
 cessing plant was not in operation, the
 pumps  drew down the manhole liquid
level so that the lagoon water flowed
back into the manhole and was pumped
to the overland flow plots. During the
last stages of  the  project, the pumps
were removed from the manhole and
suspended in the lagoon, to avoid pump
clogging when the pretreatment  facil-
ities failed This resulted in only lagoon
effluent being  pumped  to the overland
flow plots.
  Two overland flow plots were opera-
ted in parallel. The influent was applied
through a  low pressure pipe across the
top of the  slope  The effluent was col-
lected in a ditch across the foot of the
plot and piped through the V-notch weir
into  the  batch chemical   treatment
lagoon  When the lagoon became full, a
floating mechanical aerator was used to
mix the lagoon contents. Chemical solu-
tions of sodium hypochlonte for disin-
fection, followed by alum for phosphate
and suspended solids reduction,  were
added at a point near  the aerator for
mixing   After  chemical addition  was
completed  and  large   floe particles
observed, the aerator was shut off and
the contents of the lagoon were allowed
to settle
  A pump connected to a floating inlet
was used  to  discharge the  treated
supernatant into the rapid infiltration
area The rapid infiltration area provided
for final effluent polishing  and water
disposal
 Overland Flow Plots
  The overland flow plots were unique
 and warrant additional consideration
 The site was located on  a sandy loam
 soil  (U S. Soil  Conservation Service -
 Oshtemo Series), with a permeability of
 5 to 1 5 centimeters per hour (cm/hour)
 Since this high permeability would have
 resulted in rapid infiltration  instead of
 the desired overland flow, the site was
 modified to allow overland flow treat-
 ment
  The site was first stripped of topsoil,
 then graded to provide a 4% slope down-
 hill and was leveled across the direction
 of the slope. Bentonite  was applied at a
 rate of  5 kilograms per  square  meter
 (kg/m2) to the  graded subsoil and was
 mixed in to a  depth of 5 centimeters
 (cm)  A  1 5-cm layer of topsoil then was
 carefully replaced above  the bentonite
 seal and grass was planted A mixture of
 Kentucky 31 fescue and  perennial  rye-
 grass was used
   Construction costs for the overland
 flow treatment  plots of 031 hectares
were  about $10,000 or  $32,000  per
hectare in  terms of September 1978
dollars This included the cost of earth-
work, bentonite, grass, pumps, and pip-
ing but did not include the cost of  the
land  A detailed  description  of these
costs  and the plot  construction tech-
nique are included in the final report.


Conclusions
  The discussion of conclusions  has
been  divided into four sections F:irst,
the overland flow plots are discussed
primarily from a consideration of  the
hydraulics   The  performance  of  the
overland flow plots, or effectiveness of
treatment, is discussed in the following
two sections, divided according to warm
weather and cold weather operations
The final section  is  a discussion of the
batch chemical treatment  lagoon
Overland Flow: Hydraulics
  The  plots were  approximately 80
meters (m) long  This is unusually long,
about two to three times longer than
most other overland flow plots The
additional length was provided because
the wastewater strength was expected
to be several times greater than actually
occurred. The mam  disadvantage was'
the  high hydraulic  loading  per unit
width of the plot  For example,  if two
plots were equal in area, but one was
one-third as long, the longer plot would
have three times as much water flowing
along a unit width of the surface As a
consequence of the high hydraulic load-
ing, the grass at the top of the plots died,
and that area served ma inly to distribute
the flow
  Shortcircuitmg was most prevalent in
the area without grass cover, but also
occurred in the downhill grass-covered
areas due to surface irregularities An
effective method of control was devel-
oped Small earthen dikes, about 1 5 cm
high and 30 cm at the base, were con-
structed at  45° or less  to the desired
downhill direction of flow. Most dikes
were 2 m  to 5  m long and were con-
structed during wastewater application
to redirect the flow in the desired direc-
tion
  Another unique feature of the plots
was the void space m the topsoil above
the bentonite seal, which needed to be
filled with water prior to any overland
flow effluent. The  flow rate downhill
through the topsoil layer was very low
Typically, it took about two days of con-
tinuous application to fill the void spacjB

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 and for effluent to begin flowing from
 the plot  After that, wastewater flowed
 along the ground surface with detention
 times of about 3 hours to 4 hours, much
 like sites with impermeable soils
   Early in the study,  the plots were
 loaded, similar to other overland flow
 sites, 8 to 10 hours per day 5 to 7 days
 per week This cycle resulted  in even
 more serious problems at the top of the
 plot The area was almost always satur-
 ated and little effluent was generated
 The lower portions  of the plots were
 underloaded and  often too dry
   Later in the study, a different cycle
 was adopted  This cycle provided con-
 tinuous application for three to five days
 followed by two or three days of drying,
 and resulted in hydraulic loadings of 14
 to 34 cm/week The continuous loading
 for several days followed by extended
 drying periods proved to be an effective
 method of operation
   Effluent flow rates, measured during
 periods  of   wastewater  application,
 varied from  zero, when the plots were
 dry and evapotranspiration was high, to
 rates greater than influent flow rates
 The high effluent flow rates occurred
 during periods of rainfall and snowmelt
 Table 1 was prepared for one of the plots
 which had total flows available for all
 four  seasons  Averaged over  those
1 longer time  periods,  total  effluent flow
 was about 40%  of  the total influent,

 Table  1.  Overland Flow Water
           Balances

 Total
 Seasonal
 Water
 Volume,
 m3       Fall    Win    Spr   Sum

 Waste-
 water
 applied   900   580   1700   1080

 Precipita-
 tion       90    50    270     50

 Total
 Influent   990   630   1970   1130

 Effluent   410   470    780    500
                              except  during  the winter  The  high
                              winter effluent flows resulted from low
                              evapotranspiration rates, because the
                              fields were snow covered, and low infil-
                              tration rates, because the ground was
                              frozen
                              Overland Flow: Warm
                              Weather Treatment
                                Waste  production   at  the  Culver
                              processing  plant was determined on
                              several different days with the average
                              rates shown in Table 2. The USEPA ef-
                              fluent limitations  proposed for 1983
                              duck processors, in terms of averages of
                              daily values for 30 consecutive days, are
                              shown in Table 3 Also included in Table
                              3, are estimates of maximum concen-
                              trations in the Culver wastewater if it is
                              to meet those proposed limitations

                              Table 2.   Wastewater Characteristics
                              Item
                                                  Waste
                                    Concentration production
                                        mg/l     per duck
                              Wastewater
                              Volume

                              5-Day Biochem-
                              ical Oxygen
                              Demand

                              Chemical
                              Oxygen
                                                 70.4 liters
                                        256     18   grams
Demand
Suspended
So/ids
Total Kjeldahl
Nitrogen
398

170

28
28

12

2
grams

grams

grams
 Effluent
 as percent
 of total
 influent
40
75
40
45
  During  all warm-weather  periods,
and  under widely varying wastewater
application  schemes  (i e ,  both  8-16
hours/day at 9 to 25 cm/week, and 3 to
5 days continuous application  followed
by 2 to  3  days drying  at 14  to  34
cm/week), the overland flow plots alone
met  the limitations  shown in  Table 3,
except when algae problems were en-
countered Effluent concentrations of 5-
day  biochemical  oxygen   demand
(BODs), suspended  solids and oil and
grease during warm-weather operation
were all typically about one-half of the
limitations shown m Table 3  Table 4
shows average effluent values for the
fall and summer periods  The  summer
period has been further divided to show
                                                               the effect of high influent algae concen-
                                                               trations  During  the  early  summer
                                                               period  there was no substantial influ-
                                                               ent algae  However, algae produced in
                                                               the lagoon  resulted m high influent
                                                               algae concentrations during the mid-
                                                               summer  periods, and  a  reduction  in
                                                               effluent quality The poor quality efflu-
                                                               ent was  almost a direct  result of the
                                                               presence  of  influent algae  Effluent
                                                               samples which were filtered to remove
                                                               algae,  exhibited  characteristics very
                                                               nearly  the same as occurred during
                                                               periods when algae was not present m
                                                               the influent
                                                                 Effluent analyses were made during
                                                               several short  intense rainfall  periods
                                                               when  wastewater was  being  applied
                                                               and effluent was being produced Al-
                                                               though flow rates increased, no sub-
                                                               stantial change in effluent concentra-
                                                               tions was observed
Overland Flow: Cold
Weather Treatment
  Cold weather treatment refers to the
winter and early spring periods when
temperatures averaged -6°C and  5°C,
respectively Soil temperature appeared
to be the most important variable affec-
ting effluent  quality  The presence or
absence of snow cover was very impor-
tant  m determining  the  effect of air
temperatures on soil temperatures.
  Operation  was  begun  during  the
coldest month  of  the  year, January,
after two months of no operation At the
beginning of the January operation, the
overflow plot  was covered with snow at
a depth of about 1  m The wastewater
applied was either raw wastewater at
about 20°C or lagoon effluent at about
5°C All wastewater was applied during
the day (8 to 16 hours) and the plot was
allowed to rest during the night. As a
result of  the daily loading, the soil was
saturated  all  the  time because the
nighttime drying periods were insuffic-
ient to remove the water Also, hydrau-
lic loading rates were  high, about 15
cm/week,  which probably contributed
to reduced effluent  quality  In retro-
spect,  the cyclic  operation  adopted
during the following summer, continu-
ous application for  several  days fol-
lowed  by several  days  of rest, would
have been a better choice
  Air temperatures did not exceed 0°C
during the first  three weeks after start
up However, the snow cover across the
top 1 5 m of the plot was melted by the
influent wastewater, and temperatures

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Table 3.
Effluent Limitations
Item
5-Day Biochemical
Oxygen Demand
Suspended Solids
Oil and Grease
Ammonia Nitrogen
Proposed 1983
Limitation
0.39 kg/kkg LWK*
046 kg/kkg LWK
026 kg/kkg LWK
4 0 mg/l
Estimated Concentrations
for Culver Duck Faring
33 mg/l
39 mg/l
22 mg/l
4 mg/l
*L/ve weight killed kg/kkg LWK is equivalent to lb/1000 Ib LWK.
\Based on 70 liters/duck wastewater production, 50% water loss during overland flow
 treatment and a live weight of 3 kilograms per duck
Table 4.     Average  Warm Weather
            Overland Flow Effluent
Wastewater               Summer
Characteristic         Fall Early  Mid

5-day Biochemical
Oxygen Demand,
mg/l                7     16    38b

Suspended So/ids,
mg/l                n.a.   12    45C

Total Nitrogen3, mg/l
as N                 4     6    11

Total Phosphate,
mg/l as P            2.2   2.3

Oil and Grease, mg/l  n. a.   7
aSum of organic, nitrite, nitrate,  and
 ammonia nitrogen.
bWhen filtered to  remove  algae,  the
 average was 17 mg/l
c These suspended so/ids were primarily
 algal cells

were observed as high as 4°C m the top
5 cm of soil. Below that warmer soil, the
ground remained frozen  Shortly after
each period  of wastewater  application
ended, ice formed across the top 15-m
area  However,  during wastewater
application no freezing occurred even
when  air  temperatures dropped to
-15°C, except occasionally when the
colder   lagoon   effluent was  being
applied
  Below the snow cover, a thin layer of
soil remained at temperatures between
0°C  and  2°C  Below that warmer soil
layer, the soil remained frozen. Overthe
                            lower one-third of the plot, there was a
                            layer of slush about 2 cm to 5 cm deep
                            The effluent temperatures were never
                            observed to be greater than  1°C  and
                            typically were  less than 0 5°C
                              During that period of snow cover, the
                            start up period, the BOD5 in the effluent
                            gradually  decreased from  about  240
                            mg/hoabout 95 mg/l Cold weather did
                            not prevent microbial activity  Clusters
                            of what appeared to be white fungi were
                            observed The fungi probably dominated
                            other microbes due to their ability to
                            adapt to  the cold, and their ability to
                            extend hyphae to the water surface to
                            obtain  oxygen Psychrophilic fungi are
                            known to grow and  oxidize carbon in
                            temperatures near and lower than 0°C
                              The fairly rapid improvement of BOD5
                            observed after start up, was  probably
                            due to increases in microbial population
                            and not increases in oxidation rates of
                            individual microbes It is  unclear how
                            much greater  the improvement  would
                            have been  if the snow covered  condi-
                            tions had continued for a longer  period
                            of time  However,  warmer weather,
                            including ram, occurred and the snow
                            cover was  lost. Effluent  BODs  finally
                            reached  effluent  limitations  shortly
                            after the  snow melted
                              After  the snow  melt  period,  the
                            average air temperature  was 4°C for
                            about one month During that period, air
                            temperatures  fluctuated  from  above
                            freezing  to below  freezing.  Effluent
                            BOD5 values were very sensitive to air
                            temperatures  A fairly linear  relation-
                            ship  between  air temperatures  and
                            effluent BOD5  values was observed In
                            general, effluent BOD5 values did not
                            meet effluent limitations atairtempera-
                            tures below 0°C, just met them atO°Cto
                            5°C, and were well within  the limita-
tions   above  5°C   One  interesting
observation  was made as average air
temperatures  continued  to increase
Following a  week when daily mean air
temperatures  averaged  11 °C,  mean
daily temperatures dropped to -2°C on
one day and -6°C on the  next  Effluent
BOD5  increased from  the  warmer
weather values of about 10 mg/l to over
50 mg/l  on  the second day of the cold
period That  increased sensitivitytocold
temperatures  after  a period of warm
temperature  probably  was  due to a
change in the soil microbial population.
Mesophilic bacteria and other moderate
temperature microbes probably gam the
ecological advantage during the warm
spell  The ability of the newly dominant
mesophilic microbes to oxidize orgamcs
was seriously  hindered in sub-freezing
temperatures
  Effluent suspended solids concentra-
tions generally met" effluent limitations
during  the   cold   weather  period
Nitrogen  removals were generally poor
during that same period. A major spill of
anhydrous ammonia from  the  plant
refrigeration  system into the waste-
water also seriously interfered with the
nitrogen study Some nitrogen removal
did occur during  the  cold  weather The
mechanisms of nitrogen removal were
uncertain.
  During snow melting periods, effluent
BOD5,  suspended solids and ammonia
concentrations were  all relatively high
However,  effluent from  the  adjacent
overland flow plot, which had received
no wastewater during the winter, was
essentially the same as the  effluent
from the plot which had operated during
the  winter. The total mass of  effluent
pollutants associated with  the melted
snow   was  less  than the  total  mass
which would have been applied in about
3 crn  of a mixture of raw wastewater
and lagoon effluent.
  The  average effluent characteristics
are  shown  in Table 5 for  the winter
period, when snow cover existed and
spring, a time  after  snowmelt  had
occurred


Chemical Batch  Treatment
Lagoon
  Overland flow treatment resulted in
very  little phosphate reduction during
cold weather and warm operation  How-
ever, effective phosphate reduction was
provided  during  chemical batch  treat-
ment  in the lagoon located at the foot of
the  overland  flow  plots  Disinfection
was also provided in that lagoon.

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 Table 5.    Average   Cold   Weather
             Overland  Flow  Effluent
 Wastewater
 Characteristic        Winter  Spring
 5-day Biochemical
 Oxygen Demand,
 mg/l                 135     22

 Suspended Solids,
 mg/l                  40     15

 Total Nitrogen", mg/l
 as N                  54     11

 Total Phosphate,
 mg/l as P              2826

 Oil and Grease, mg/l    7     n.a
 *Sum of organic,  nitrite, nitrate,  and
  ammonia nitrogen.

   A complete ana lysis was made on one
 batch  treatment  The treatment  in-
 cluded addition of a sodium hypochlo-
 rite  solution resulting in a  5  mg/l
 chlorine dosage. Mixing was provided,
 using the floating mechanical aerator.,
; during chlorine addition and was con-
 tinued for 30 minutes  Chlorine resid-
 uals both total  and  free,  were greater
 than 3 mg/l after the 30-mmute mixing
 period  An  alum solution  was  then
 added to the mixing lagoon over a period
 of one hour,  which resulted  in  an
 aluminum dosage of 50 mg/l  After 10
 minutes more of mixing, the  aerator
 was shut off, and the lagoon contents
 allowed to settle for 1 5 hours  Table 6
 compares  the  lagoon contents before
 and after treatment.


 Recommendations
   Overland flow should be considered
 as a wastewater treatment alternative
 at sites with*permeable as well as im-
 permeable  soils Application  of  more
 than 5  kg/m2 of bentonite is recom-
 mended to ensure a better percolation
 barrier at future sites.
   In systems requiring increased phos-
 phate removal disinfection, or a greater
 assurance of suspended  solids reduc-
 tion, a small  lagoon should be construc-
 ted to collect the overland flow effluent.
 This provides opportunity,  using simple
 batch  chemical  treatment  and
 controlled discharge, to improve efflu-
     quality. This relatively inexpensive
treatment is well suited for use in con-
junction with overland flow.
   Research should be  undertaken to
determine  optimum wastewater appli-
cation cycles  Experience  at  the  site
suggests that 24 hours per day applica-
tion for several days, followed by two or
three days of  drying,  is a cycle which
deserves future investigation.
   Winter storage is needed However,
winter operation   needs to be investi-
gated further This investigation should
concentrate on overland flow treatment
on snow covered  plots,  because sub-
stantially higher  quality effluents are
likely with the snow cover to protect the
soil from very low and highly fluctuating
temperatures  The study indicated that
some degree of treatment is possible in
the winter, but that loading rate must be
reduced, and unsatisfactory  effluent
returned for further treatment. Winter
wastewater application is recommend-
ed to  develop a  microbial population
capable  of treating  the wastewater
immediately following the spring thaw.
   Further investigations are necessary
to  determine  the  effect  of  sudden
decreases in temperature in late fall and
early spring. It may be necessary to stop
Table 6.    Lagoon  Chemical Batch
            Treatment
 Wastewater
 Characteristic
 Treatment
Before  After
5-day Biochemical
Oxygen Demand,
mg/l                 38
Suspended Solids,
mg/l

Ammonia Nitrogen,
mg/l

Nitrate plus Nitrite
Nitrogen, mg/l

Total Kjeldahl
Nitrogen, mg/l

Total Phosphate,
mg/l as P
 29      20
  72     70
  0.3     0.1
 12.9     8.5
  2.8     0.4
Inorganic Phosphate,
mg/l as P              2.1      0.1
                    wastewater  application during these
                    short cold spells
                      Application of lagoon wastewater to
                    an overland flow plot should be avoided
                    if possible. Operators of systems using
                    storage lagoons prior to overland flow
                    treatment are recommended to by-pass
                    these lagoons during peak algae grow-
                    ing periods.  Removal of sufficient grit
                    and solids to prevent pump and nozzle
                    plugging  is  recommended when
                    lagoons are  by-passed or raw waste-
                    water is applied directly to overland flow
                    plots
Coliforms

pH
  n.a.     0

  n. a.     6.5

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Lloyd H Ketchum, Jr. and Robert L. Irvine are with the University of Notre Dame,
  Notre Dame, IN 46556; Arthur J. Cunningham is with the New Hampshire
  Water Supply and Pollution Control, Concord, NH 0330 1; Jack L. Witherowis
  the EPA author as we/1 as the EPA Project Officer (see below).
The complete report, entitled "Overland Flow Treatment of Poultry Processing
  Wastewater in  Cold Climates," (Order No. PB 81-213 225; Cost: $12.50,
  subject to change) will be available only from.
        National Technical Information Service
        5285 Port Royal Road
        Springfield, VA 22161
        Telephone. 703-487-4650
The EPA Project Officer can be contacted at.
        Robert S. Kerr Environmental Research Laboratory
        U.S Environmental Protection Agency
        P.O. Box  1198
        Ada,  OK 74820
                                                                          '. US GOVERNMENT PRINTING OFFICE 1W1 -757-012/7273

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Environmental Protection
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Information
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