United States
 Environmental Protection
 Agency
 Industrial Environmental Research
 Laboratory
 Cincinnati OH 45268
 Research and Development
 EPA-600/S2-84-023 Mar. 1984
 Project  Summary
 Sulfide  Precipitation  of Heavy
 Metals:  Effect  of Complexing
 Agents
 D. Bhattacharyya and Y. Ku
  Sulfide  precipitation behavior of
heavy metal ions  in the presence of
chelating  agents was  evaluated in
terms of type of metal ion, chelating
agent type and concentration, pH,
sulfide  dosage and  reaction time.
Theoretical solubilities of metal sulf ides
were calculated for the various metal-
sulfide-chelating agent systems and
compared with the experimental values.
Experiments with Zn and Cd showed
that  Ksp's  of ZnS and CdS of fresh
precipitates were one or two log cycles
higher than the aged precipitate Ksp
values. With metal sulfides of  higher
solubilities (such as, zinc and nickel),
high  concentrations (greater than 10
mg/l) of strong chelating agent (such as
EDTA) substantially reduced the extent
of sulfide precipitation.
  For sparingly soluble metal sulfides
(such as, copper and cadmium), the
effect of  the  chelating agent  was
insignificant; residual concentration of
less 1mg/l was obtained even with the
strong chelating agent at a pH  4. The
presence of a  high concentration of
citrate caused partial dissolution of
copper sulfide. NiS precipitation was
effective only for short reaction times
(less than 5 minutes) or in the absence
of oxygen. CdS  precipitation  was
effective even  in  the presence  of a
strong chelating agent,  but the removals
of cadmium were interfered with in the
Ni-containing-systems. Continuous
experiments were  also conducted to
evaluate the effectiveness of the settling
operation  and  to  establish selective
metal sulfide separation.
  This Project Summary was developed
by EPA's  Industrial  Environmental
Research Laboratory,  Cincinnati,  OH,
to announce key findings of the research
project that is fully documented in a
separate report of the same title (see
Project Report ordering information at
back).

Introduction
  Process wastewaters containing heavy
metals result from metal finishing and
production  operations and certain inor-
ganic chemical manufacturing operations.
Depending  on their origin, these process
wastewaters may also contain chelating
agents such as EDTA  and citrate. The
effective removal of heavy metals or their
selective recovery, even at high pH, is
often not  feasible with conventional
hydroxide precipitation,  the method most
often used  for treating  industrial waste-
waters.
  In  hydroxide precipitation  of heavy
metals, the metals are  removed by
adjusting the pH of the  wastewater with
lime  or caustic soda  until the metals
exhibit minimum solubilities. The metal
hydroxides can be removed by flocculation
and sedimentation. Some common limit-
ations of the hydroxide  process are:
   - Hydroxide precipitates tend to reso-
    lubilize if the solution pH is increased
    or  decreased from their  minimum
    solubility values; maximum removal
    efficiency may not be achieved
    unless the pH is controlled within a
    narrow range.
   - The theoretical minimum solubility
    for different metal hydroxides occurs
    at different pH values; hence, the
    removal  of metals by hydroxide
    precipitation of mixed metal wastes
    may not be effective.
  Sulfide precipitation  is  an  effective
alternative  to hydroxide precipitation for

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removing various heavy metal ions from
industrial wastewaters. An earlier bench-
scale study evaluated the effectiveness of
sulfide  precipitation  using  Na2S. That
investigation showed that  high  metal
removal efficiencies can be achieved.
Although several literature citations
mention the effect of chelating agents on
sulfide precipitation, no quantitative
research of this effect has been reported
under  various operating conditions  for
metal sulfide precipitations. Since metal
chelates are highly dissociated at low pH
values, effective metal removal by sulfide
precipitation is possible even at pH 3-4.
Selective precipitation of heavy metal
sulfides can be obtained by the addition of
proper chelating  agents.
  The  overall  objective of the present
investigation was to establish the extent
of precipitation of selected heavy metals
with sodium sulfide in the presence of
several commonly used chelating agents.
Extensive bench-scale experiments were
conducted with several synthetic systems.


Dissociation of Complexing
Agents
  In the absence of metal ions, complex-
ing  agents  may form  complexes with a
proton, H+. The stepwise acid dissociation
constants for polyprotinated ligands can
be expressed as follows:
  HnL    H + Hn-lL                  (1)
  Hn-!L   H + Hn-2L                  (2)
   HL      H + L                   (3)
in which "L" stands for ligand.
  The corresponding stepwise dissociation
constants for several complexing agents
are shown Table 1. Using these stepwise
dissociation constants, a set of simulta-
neous equations can be  solved to obtain
the distribution of various species in the
solution under different experimental
conditions. A typical species distribution
of EDTA is computed and shown in Figure
1 as a function of pH.
  The equilibrium concentration of  all
species present in  a  metal-sulfide-
chelating agent system can be calculated
by  solving  a series of simultaneous
equations comprised of equations  for
materials balance, solubility  product of
metal sulfide, expressions of species, pH,
total metal  concentration,  total  sulfide
concentration and total complexing agent
concentration. A computer program was
designed  to solve these equations and
calculate  the equilibrium concentration
of all species. The  equations and the
                                         Table 1.    Acid Dissociation Constants of Chelating Agents
                                                                              Dissociation Constant (at 25C)
Complexing
Agents
Citric acid
Cyanide
DCTA
EDTA
Gluconic acid
HEDTA
NTA
Oxalate
Tartrate
pK,
3.00
9.20
2.51
2.07
2.30
2.40
1.89
1.40
2.90
pKz
4.40
---
3.60
2.75
4.28
5.40
2.49
3.80
4.10
pK3
6.10
...
6.20
6.24
9.67
10.00
9.73
...
—
pK*
16.00
...
11.78
10.34
...
-_.
...
.__
—
                                            100

                                         Figure 1.    Distribution in various species of EDTA in aqueous solution as a function of pH.
                                         computer program are presented in the
                                         final report.

                                         Metal-Sulfide-Chelating Agent
                                         Equilibrium Calculations
                                           Using the  calculation  procedure re-
                                         ferred to above, the theoretical values
                                         of residual metal concentrations,  CM,
                                         were calculated for various zinc-sulfide-
                                         chelating  agent systems at a sulfide
                                         dosage of  1.0x and 8.9x10"3M chelating
                                         agent concentration and  plotted as  a
                                         function of pH. Zinc residual concentra-
                                         tions are always higher in the presence of
                                         chelating  agents, indicating that  the
                                         formation  of  zinc chelates reduces the
                                         extent of ZnS precipitation. For example,
                                         at pH 8, zinc residual concentration is
                                         about 7 mg/l in the presence of the
                                         strong  chelating agent EDTA, whereas
residual concentration is about 10 :> mg/1
in the absence of any chelating  agents.
The final report shows the residual zirc
concentrations in the presence of other
chelating  agents of  various degrees of
metal chelate stability.  Weak chelating
agents such as tartrate and gluconic acid
had little influence on the residual con-
centration.  The interference of these
chelating  agents, in  terms of reduction
of sulfide precipitation, increases  in order
of: tartrate < gluconic acid  < citrate <
NTA (nitroloacetic acid) < HEDTA (hydro-
xyethyl-ethyine-diaimetriacetic  acid) <
EDTA.
   The   pH  effect  on the residual zinc
concentration with Ksp values is shown in
the final report. At low pH, removal of zinc
is not complete because the protonation
of sulfide tends to form  HzS. and part of
the HzS  may be lost into the air, thus

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decreasing the extent of ZnS precipitation.
In alkaline conditions,  the metal forms
stable chelates because of the deprotona-
tion of  the chelating-agent.  For pH 10,
formation of  metal hydroxide chelates
becomes another competing reaction in
the  presence  of  some weak chelating
agents and increases residual concentra-
tion. But with strong chelating agents, no
significant residual change occurs  even
at high pH because of the formation of
highly stable chelates.
  The effect of soluble sulfide concentra-
tion (Si) on sulfide precipitation is also
shown in the final report. At a higher ST,
the  residual  concentration is always
lowered. The ST value  increases as the
sulfide  dosage exceeds 1.0x. A  higher
dosage of sulfide  always lowers the
residual metal concentration due to com-
mon ion effect.
  Equilibrium calculations for Cu, Cd and
Ni sulfide precipitation were made in the
presence of various chelating agents.
Because of the high reactivity of Cu with
sulfide  (Ksp =  1.2x10~35), the formation
of CuS is more favorable over the formation
of copper chelates.  The effect of the
formation of  metal-EDTA chelates on
various metal  sulfide precipitation reac-
tions was also plotted. Metal sulfides
with small solubility products (Ksp),  ouch
as Cu and Cd, always have low residual
concentrations and are less influenced by
the presence  of chelating agents.

Comparison with Hydroxide
Precipitation
  In  the final report  residual metal
concentrations after hydroxide precipita-
tion with EDTA are presented for several
heavy metals. The results are considerably
higher than those for sulfide precipitation,
especially at low pH values. For example,
at pH 6, zinc does not form a hydroxide
precipitate but the residual is about 16
mg/l after sulfide precipitation.  The
difference is even greater for zinc sulfide.
In this study, EDTA, citrate, tartrate and
gluconic acid  were used  as  chelating
agents.  Among these chelating agents,
EDTA has the highest complex formation
equilibrium constants, which  indicate
that EDTA may form more stable chelates
with the zinc  ion than other chelating
agents  used  in this study.  For weak
chelating  agents such as citrate  and
tartrate, the  sulfide precipitation is
quite complete, indicating that the for-
mation of zinc chelates with  citrate or
tartrate  is much  less favorable of  zinc
sulfide precipitation.
  According to Le Chatelier's principle of
equilibrium, the residual metal concen-
tration increases with an increase in the
amount of chelating agent present in
initial feed solution. This was verified for
the  zinc—EDTA  system.  Experiments
were conducted at pH  8.0 and in  the
presence of various amounts of EDTA for
the zinc-EDTA system. The  residual zinc
concentration increased with an increas-
ing  amount  of EDTA present  in  the
solution. A high concentration of EDTA
caused severe interference in the precipi-
tation of the zinc sulfide. For example, 72
mg/l of zinc residual did not precipitate
with sulfide in the presence of 500 mg/l
of EDTA. Assumptions can be made that
about  70%  of the EDTA  formed  1:1
chelates with the zinc at EDTA concentra-
tions less than 500 mg/l and EDTA/Zn
ratios less than 1.0. In other  words, about
12.5 mg/l of soluble zinc were present
with 100 mg/l of EDTA. This assumption
agreed with  the fact  that almost no
precipitation occurred when the initial
zinc concentration was  10 mg/l and the
EDTA concentration was 100 mg/l (3.4x
10~4M). Some experiments were carried
out  for the zinc-citrate system.  The
presence of  citrate had  a negligible
influence on the precipitation of  zinc
sulfide. This  is attributed to the weak
chelation ability of citrate. The sulfide ion
competed more successfully than citrate
for the coordinative position on the zinc
ion.

Effect of pH Value
  The  species  distribution  of  different
forms of metal chelates present in  the
solution varies at different pH values. In
order to evaluate the effect  of pH on the
removal of metal by sulfide  precipitation
in the presence of chelating agents, a
series of experi ments was ca rried out at a
1.05x  sulfide dosage and various pH
values in the presence of 10 or 100 mg/l
of EDTA. The residual zinc concentrations
at various pH values appear in the final
report. Removal  of  zinc  by  sulfide
precipitation was relatively incomplete at
pH 3 because of the competition of the
sulfide ion for  H+. On  the  other hand,
protonated EDTA  species,  H
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   batch experiments. Sulfide dosage fluc-
   tuation may have been caused by some-
   what higher residuals.

   Multi-Metal System
     A mixture of Cu2+, Cd2+, Zn2+, and Ni2+
   ions was used  in this study with and
   without EDTA. Results  were  plotted
   against the residual metal concentrations
   for  batch experiments  at  the same
   operational conditions. Relatively low
   residual metal concentrations were
   obtained for copper  and zinc. However,
   removals were  not  complete for nickel
   and cadmium  ions  because  of the
   interference between these metal ions.
   Without the Ni  ion,  a  low residual
   cadmium concentration was achieved for
   a mixture of Cu2+,  Cd2+, and Zn2+ ions by
   sulfide treatment.
     Another important observation in this
   research pertained to the  sedimentation
   of metal sulfide precipitates in the sett-
   ling column. Sedimentation is one of the
   most used unit operations in wastewater
   treatment. In  this study,  a 90-cm  long
   glass column with a 7-cm diameter was
   used as a sedimentation column. At a
   feed solution  flowrate  of 100 cmVmin
   the  detention  time in the  sedimentation
   column  was  35 minutes. The  surface
   loading rate was 2.6 cmVfcm2) (min). In
   the  final report,  metal sulfide precipitate
   removals are shown as a function of the
   initial concentration of EDTA. The ZnS
   precipitate was removed quited effect-
   ively. The experimental data show that
   about 20-30% of Cu, 60-80% of Cd, and
   60-100% of Ni precipitate were removed
   by sedimentation. The presence of EDTA
   improved the sedimentation of copper
   and nickel sulfides.
          D. Bhattacharyya and Y. Ku are with the University of Kentucky, Lexington, KY
            40506-0046.
          Alfred B. Craig, Jr., is the EPA Project Officer (see below).
          The complete report, entitled "Sulfide Precipitation  of Heavy Metals: Effect of
            Complex!ng Agents," (Order No. PB 84-141 514; Cost: $14.50, subject to
            change) will be available only from:
                  National Technical Information Service
                  5285 Port Royal Road
                  Springfield, VA 22161
                  Telephone: 703-487-4650
          The EPA Project Officer can  be contacted at:
                  Industrial Environmental Research Laboratory
                  U.S. Environmental Protection Agency
                  Cincinnati, OH 45268
                                           •fr U.S. GOVERNMENT PRINTING OFFICE, 1984 — 759-015/7636
United States
Environmental Protection
Agency
Center for Environmental Research
Information
Cincinnati OH 45268
Official Business
Penalty for Private Use $300
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