United States
                   Environmental Protection
                   Agency
Environmental Research
Laboratory
Corvallis, OR 97333
                   Research and Development
EPA/600/S3-87/025  August 1988
&EPA          Project Summary

                    The Ohio  Stream
                    Regionalization Project:  A
                    Compendium  of  Results
                    Thomas R. Whittier, David P. Larsen, Robert M. Hughes, Christina M. Rohm,
                    Alisa L Gallant, and James M. Omernik
                     Regional  patterns in  terrestrial
                   characteristics  can be used  as  a
                   framework to monitor, assess and
                   report the  health  of aquatic
                   ecosystems. In Ohio, five ecological
                   regions were delineated using spatial
                   patterns in land-surface  form, land
                   use,  soil  and  potential natural
                   vegetation.  We evaluated  this
                   framework by studying  the  water
                   quality, physical habitat, and fish and
                   macroinvertebrate  assemblages  of
                   109 minimally  impacted  repre-
                   sentative streams. Water quality and
                   fish assemblages  showed  clear
                   regional  differences.  The highest
                   quality water and fish  assemblages
                   were consistently found in the
                   southeast ecoregion and the lowest
                   quality in  the northwest ecoregion.
                   We found  no clear regional patterns
                   in  macroinvertebrate  assemblages
                   and limited  regional patterns  in
                   physical habitat
                     This Profect Summary  was
                   developed by EPA's Environmental
                   Research Laboratory, Corvallis, OR, to
                   announce key findings of the
                   research project  that is  fully
                   documented in a separate report of
                   the same  title (see Project Report
                   ordering Information at back).

                   Introduction
                     To implement  the mandates  of the
                   Clean Water Act  of 1977 (CWA),  many
                   state environmental protection agencies
                   have relied on statewide chemical criteria
                   that presumably  protect  fish, shellfish,
                   and wildlife. These agencies increasingly
 face a need to address the goals of the
 CWA  more directly,  through  regional
 chemical and biological objectives for
 water quality management.
   The  Ohio  Environmental Protection
 Agency (OEPA) saw a need to determine
 what was reasonably attainable in  Ohio
 streams.  OEPA  also  recognized
 differences in streams in various parts of
 the state.  These concerns  led  to
 cooperative research between OEPA and
 the  USEPA  to  evaluate  a land
 classification for  identifying  regional
 patterns in aquatic ecosystems  and
 attainable  physical,  chemical  and
 biological conditions within them.

 Ecoregion Concept
   An  important premise in our work is
 that streams reflect the characteristics of
 the land they drain. Streams in areas of
 relatively homogeneous land should be
 relatively similar to each other. Thus,
 regional  patterns   in  terrestrial
 characteristics can  be  used  as a
 framework  for  studying  aquatic
 ecosystems.  In Ohio,  five ecological
 regions were delineated  using spatial
 patterns in land-surface form, land use,
 soil, and potential natural vegetation.
   Sampling sites  were chosen at 109
 locations throughout Ohio that met three
 criteria: 1) The watersheds and streams
 were minimally impacted by  human
 activity, 2) The streams, watersheds and
 sites were representative of their regions,
 and  3)  The watersheds above the
 sampling site were wholly contained
 within  one ecoregion  (some cross-
 regional boundary  sites were  also

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studied).  This  sampling scheme  helps
assure  that  data  from  these  sites
represent attainable conditions for  each
region. The OEPA sampled these sites in
1983-84  for  fish, macroinvertebrates,
water quality, and  physical habitat.

Regional Patterns
   The clearest regional  patterns  were
found in  the  fish  assemblages and the
water quality attributes.  The  Index of
Biotic  Integrity (IBI)  which expresses
ecological  health  by assessing  fish
assemblages, was highest  in  southeast
Ohio  (particularly in  the  Western
Allegheny Plateau). The  lowest values
were  in northwest Ohio  (mostly in the
Huron/Erie  Lake Plain), Figure  I. These
two regions also have the most distinctly
different landscapes; the northwest is flat
and  predominantly  used for row  crop
agriculture, the  southeast is a  dissected
plateau consisting of woodland mixed
with some cropland and  pasture.  The
other (transitional) regions had generally
intermediate IBI values. This pattern was
found for several other measures
generally  considered  indicative of the
quality of fish assemblages, e.g. species
richness,  diversity,  and   number of
species and proportion of fish intolerant
of siltation and low dissolved oxygen.
   Similar patterns  in  water quality
occurred. The  highest quality was  in
southeast Ohio and the  lowest in  the
northwest. The streams in the Huron/Erie
Lake Plain were nutrient and mineral rich.
Figure 2  summarizes the  relationship
between water quality of  the  sites and
their regions.
   There  were  only  slight  regional
patterns in the physical habitat attributes.
Streams in the southeast portion of  Ohio
tended to have more  instream cover for
fish (logs, boulders, undercut banks) and
coarser  substrate  materials  (cobbles,
rocks and  boulders), than the  other
regions.  Macroinvertebrate  assemblages
showed almost no regional pattern.

Conclusions
   There  are clear regional patterns in
Ohio's  stream  ecosystems. While the
ecoregion classification  does not  fit
perfectly  for any single component of
aquatic ecosystems,  it does  provide  a
useful geographic framework to monitor,
assess,  and  report  on  the  status  of
aquatic  resources.  The  regions,
combined  with  the sampling strategy
 outlined here, can be used  to determine
a  range  of  reasonable  regional
expectations for  attainable water  quality
 and biological communities.
   Ohio's  current,  highly-detailed
stream  standards  based  on  several
categories  of biological  uses  and
biological  criteria  are  being  revised  by
the  OEPA  based  on  reasonably
attainable  conditions.  The  OEPA is
combining the data  and  methods of this
study with their extensive  knowledge of
Ohio  stream  conditions to determine
regional background water quality.  For
example,  based  on these   results,
attainable  phosphorus  levels  for  the
Western Allegheny Plateau  could  be
<0.05 mg/l; in the Huron/Erie Lake  Plain
a  more realistic goal might be <0.15
mg/l.  These  levels  are  based  on  this
study  only  and will be  subject  to
revision.
   o
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Ecoregion
                                                       IP
WAP
 Figure 1.
Relationship between the Index of Biotic Integrity and ecoregions in Ohio. Each
boxplot includes: median {the circle), interquartile range (height of the wide box).
10 and 90 percentiles (height of the narrow box), relative sample size (width
of the box), and range.

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Figure 2.
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                         Nutrient Richness (PCA 1)

       Regional patterns in nutrient richness and ionic strength variables indicated by
       principal component axis I scores for each. Areas enclosed indicate hypothesized
       attainable water quality for each region.

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  Thomas R. Whittier, Robert M. Hughes, Christina M. Rohm, and Alisa L Gallant
    are with Northrop Services, Inc., Corvallis, OR 97333. The EPA authors David
    P. Larsen (also the EPA Project Officer) and James M. Omernik are with the
    Environmental Research Laboratory, Corvallis, OR 97333.
  The complete report,  entitled "The Ohio Stream Regionalization Project:  A
    Compendium of Results," (Order No. PB 88-204  2271 AS; Cost: $14.95,
    subject to change) will be available only from:
       National Technical Information Service
       5285 Port Royal Road
       Springfield,  VA 22161
       Telephone:  703-487-4650
  The EPA Project Officer can be contacted at:
       Environmental Research Laboratory
       U.S. Environmental Protection Agency
       Corvallis, OR 97333
United States
Environmental Protection
Agency
Center for Environmental Research
Information
Cincinnati OH 45268
Official Business
Penalty for Private Use $300

EPA/600/S3-87/025

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