United States
Environmental Protection
Agency
Municipal Environmental Research
Laboratory
Cincinnati OH 45268
Research and Development
EPA-600/S2-81 -228  Dec. 1981
Project  Summary
Performance of Trickling
Filter  Plants:  Reliability,
Stability  and  Variability

Richard Haugh, Salar Niku, Edward D. Schroeder, and GeorgeTchobanoglous
  Effluent  quality  variability from
trickling filters was examined in this
study by statistically analyzing daily
effluent BODs and suspended solids
data  from  11  treatment  plants.
Summary statistics (mean, standard
deviation, etc.) were  examined  to
determine the general characteristics
of those data. Distributions of most
effluent data were skewed to the right,
and  daily  suspended  solids  data
generally exhibited  more variation
than daily BODi data.
  Five probability  distribution
functions, chosen through experience
and the literature, were tested to de-
termine which would be best used to
describe daily, 7-day average and 30-
day  average effluent data distribu-
tions. Three  distributions (the two
parameter empirical, the gamma, and
the log normal) were found to  be
adequate, with the log normal being
preferred because of ease of applica-
tion.
  Daily effluent BODs and suspended
solids data were found to contain both
random and nonrandom components.
Weekly cycles were found in about
half of the plants studied, and signifi-
cant month-to-month  variation  in
effluent quality was found in every
plant. Effluent BOD and suspended
solids concentrations were higher in
winter than in summer when pooled
data from all plants were examined.
  Multiple regression  analysis was
used to determine  the effects  of
various process parameters on efflu-
ent  quality.   In  general,  primary
effluent BODs and suspended solids
concentrations  and  wastewater
temperature had the greatest effect.
Variation due to measurement error
was estimated to be 5 to 78 percent
and  11 to 78 percent for effluent
BODs and suspended solids values.
respectively.
  Methods for incorporating statis-
tical  concepts into trickling  filter
design and operation are discussed.
  This Project Summary was develop-
ed by EPA's Municipal Environmental
Research Laboratory, Cincinnati, OH,
to announce  key findings of the
research project that is  fully docu-
mented in a  separate report of the
same title (see Project Report ordering
information at back).


Introduction
  As discharge  requirements  have
become more  stringent,  it has become
necessary to include concepts of vari-
ability into the theory,  design, and
regulation  of wastewater treatment
plants. For example, limitations on the
average daily and weekly concentrations
of pollutants have made it necessary not
only to insure that average performance
is within discharge limits, but also to
reduce  the amount  of  variation  in
performance.
  A number of recent studies  have
examined effluent variability in waste-
water treatment  plants,  but most of
these studies  have been concerned

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solely  with  activated  sludge  plant
performance. The purpose of this study
was to investigate and characterize the
nature and sources of  variability of
effluent quality in trickling filter waste-
water treatment  plants.  Five-day
biochemical oxygen demand (BODs) and
suspended solids (SS) concentrations
are the effluent quality parameters that
are examined.
  Most theoretical and laboratory work
on  wastewater  treatment  processes,
and practically all studies of data from
full scale  treatment plants, have been
based  on deterministic,  steady state
concepts.  Recently,  however,  dynamic
and stochastic models have been devel-
oped for both the activated  sludge and
trickling  filter processes.  Laboratory
studies have been done on the activated
sludge process under variable loading
conditions, and treatment plant influent
and effluent data have  been studied
using sophisticated stochastic models.
  There exists a need to incorporate the
concept of variability in virtually every
area of the wastewater treatment field.
Numerous authors  have argued that
stochastic concepts should be included
in  wastewater  treatment -  plant
planning, design, and regulation. In the
following  sections,  areas in  which
consideration of treatment variability is
important are.discussed.
Variability of Trickling Filter
Processes

Discharge Requirements
   Effluent  variability is  implicitly
recognized in current federal secondary
treatment standards  plant  discharge
limits. The  7-day  average maximums
allow for the fact that short term fluctu-
ations tend  to be averaged  out in the
long run.
   However,  daily, weekly  and monthly
concentration limits are not explicitly
stochastic because they set maximum
values that cannot be exceeded (without
causing a violation). These fixed limits,
especially daily maximums, are concep-
tually inconsistent  with the stochastic
nature  of   wastewater  treatment
processes and requirements should be
restated in  a probabilistic manner. An
example  of a probabilistic  standard
would be one in which the daily,com-
posite  average   BODs concentration
cannot exceed a specific value more
than two times in any consecutive 30-
day period.
Design
  Stochastic concepts are also useful in
treatment plant design. Designers must
consider the degree of variability to be
expected, as  well  as   the  average
performance, if discharge requirements
are to  be met. Currently, most design
techniques are based on steady state
assumptions and average constituent
values are used for design parameters.
One method of accommodating process
variability is to oversize the treatment
units. Another way to allow for process
variability is to determine the  expected
effluent variation based on data from
existing treatment  plants. Linear
regression models can be developed to
predict  the distribution  percentiles  of
effluent  BOD  and SS concentrations,
based on the annual mean value of each
parameter.  Working   backwards,
designers can  choose an effluent con-
centration not to be exceeded more than
a prescribed percentage of the time and
then  calculate the  required  average
value.  Using these computed average
values  as  design criteria, traditional
design methods can then be used to size
the  treatment  units.  If  the design
averages   are   achieved,  treatment
variability should be within the expected
limits.
  Possibly   a   more  cost   effective
approach to reducing the occurrence of
high  effluent  concentrations   is  to
reduce  the variability  of treatment
processes rather than improving the
average performance of these  proc-
esses. Reducing the  variability has the
effect of narrowing the distributions of
effluent values. The use of equalization
chambers  and  automatic  process
control  have  been  proposed for the
purpose of reducing effluent variability.
Equalization of influent flows reduces
loading fluctuations;  because  the treat-
ment process is operated  under loading
conditions that approach steady state
conditions,  fluctuations  in  process
performance are reduced. Effluent flow
equalization before discharge reduces
effluent quality variation directly  by
mixing  flows from different periods of
process  performance. Automatic
control  can  be used  to adjust process
parameters to  compensate for changes
in  loading  conditions   and  process
efficiently.

Fluctuations in  Trickling Filter
Performance
  Effluent quality variability in trickling
filter plants is caused by a number of
environmental, operational, and loading
factors. Factors that cause fluctuations
in trickling filter performance are dis-
cussed below.
  The wastewater flow rate through the
trickling filter controls the thickness and
velocity of the liquid film on the biolog-
ical  slime. An increase in  flow rate
increases the film thickness, which in
turn inhibits oxygen transfer through
the film. Higher liquid velocities reduce
the contact ti me for diffusion of organics
from the liquid into the slime. Thus the
overall effect of increased flowrate is a
higher filter effluent BOD concentration
for a given influent BOD concentration.
  An increase in the concentration of
organic  matter  entering  the  filter
generally  causes  an increase  in  the
filter  effluent  organic  concentration.
Increased  organic concentration
increases the mass loading rate on the
filter  and raises the concentration of
organics  in  the  liquid film. The in-
creased   concentration  of  organics
causes food and nutrients to penetrate
deeper  into  the  slime  layer,  which
increases the reaction rate per unit area
unless the maximum reaction rate has
been reached or some other factor, such
as  oxygen transfer,  has become rate
limiting.  Although the  mass removal^
rate increases, it does not increase as'
much as the increase in mass loading,
and the  net  result  is a  higher filter
effluent organic BOD concentration.
  Air and wastewater temperature both
affect treatment efficiency. A tempera-
ture increase in the liquid film and bio-
logical slime  increases transport and
reaction  rates, thus improving  treat-
ment efficiency. In conventional filters,
the supply of air (and thus oxygen) to the
microorganisms is  controlled by the
relationship  between  air and waste-
water temperature. The greater  the
difference  between air and   water
temperature,  the  greater  the   draft
through the filter.
   Other factors that affect filter opera-
tion  are  wastewater   pH  and  the
presence  of toxic  substances. The
optimal  pH  for  microbial  growth  in
conventional  biological  treatment  is
between 6.5 and 8.5. IfthepH isconsid-
erably outside this range,  treatment
efficiency  will decrease.  Toxic  sub-
stances in the influent can  also retard
biological activity and reduce treatment
efficiency.
   Trickling  filters   are  quasi-natural
biological  processes,  and  there   is
evidence   that   slime   growth-decaj|

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cycles occur locally within the filter,
independent of fluctuations in flow rate,
organic concentration or other param-
eters. It is not known whether these
cycles cause significant variations in
the overall performance of the filter.

Fluctuations in Clarifier
Performance
  The most important factors that affect
clarifier performance are the concentra-
tion   and  composition  of  incoming
suspended matter and the flow rate
through the tank. An increase in flow
rate  decreases the time available for
particles to settle to the bottom of the
tank and thus increases the concentra-
tion of particles in the clarifier effluent.
  Density, shape, and size all affect the
settling  velocity  of   particles.  An
increase in the settling velocity of the
suspended particles entering the clari-
fier  results in  an  increase  in  the
percentage of particles removed.
  Higher concentrations of suspended
matter in the clarifier influent generally
result in higher effluent concentrations
because there  is an increase  in  the
concentration of particles with settling
velocities too low to be removed.
  Flocculation and straining of particles
also affect suspended solids removal in
clarifiers, but these mechanisms cannot
be explained simply in terms of clarifier
influent strength and flow rate.
  Other factors can influence clarifier
performance.  Improperly  operated
sludge scrapers can  cause  disruptive
currents in the tank, and settled solids
that remain on the bottom of the tank
too long can  become anaerobic and
produce gas bubbles that float solids to
the water surface.

Objectives
  The purpose of this study is to investi-
gate  the  characteristics  and  some
possible sources of variability in final
effluent quality in trickling filter waste-
water treatment plants. Daily perform-
ance data have been obtained from 11
treatment  plants and  analyzed statis-
tically  to  investigate  the following
questions:

  1.   How can the distributions of efflu-
      ent BOD5 and SS concentrations
     from trickling filter plants be char-
     acterized?

  2.   Are trickling filter effluent BOD5
      and  SS   concentrations  data
      random?
 3.  If  trickling  filter plant effluent
     BODs and SS data are not random,
     do the data  follow any annual or
     weekly cycles?

 4.  What are the effects of influent,
     environmental,   and operational
     parameters on effluent variability?

 5.  What is the effect of measure-
     ment error on effluent data vari-
     ability?

  It would be worthwhile to study the
variability of each process. However,
data from between the trickling filter
and  final clarifier are  not generally
available. Therefore,  in this study only
the variability of the final effluent is
examined.


Procedures
  The data base for this study consists
of daily operation  and  performance
records from  11  trickling filter plants
(Table 1) located in six different states.
Data sets from most of the plants are 1
year in length, but the data from plants 5
and 7 are somewhat longer. All concen-
tration data are flow weighted compos-
ite values.
                                         Characterization of Effluent
                                         Distributions
                                           To characterize the effluent BODs and
                                         SS distributions for the treatment plants
                                         in this study, summary statistics such as
                                         the mean, standard deviation, and skew
                                         coefficient were computed for daily, 7-
                                         day  average  and   30-day  average
                                         effluent  concentrations   from  each
                                         plant. Also, three theoretical  and  two
                                         empirical distribution functions were
                                         chosen as possible models for effluent
                                         distributions and were tested using the
                                         Kolmogorov-Smirnov  goodness-of-fit
                                         test.  Details of these procedures are
                                         explained below.
                                         Running Averages
                                           Portions of  federal and state  dis-
                                         charge requirements for BOD5 and SS
                                         concentrations are stated  in terms of
                                         running 7- and 30-day averages. There-
                                         fore, the distributions of these averages,
                                         as well as the distributions  of daily
                                         averages,  are  of interest and will be
                                         investigated in this study. Trickling filter
                                         eflfuent data  are  not random,  and it
                                         cannot be assumed that running aver-
                                         age  distributions  are approximately
                                         normal. Therefore, the distributions of
Table 1.   Trickling Filter Treatment Plants Studied


               Location        Period of Data
 Plant
Number
Type of
Process
 Average
Flow (mgd)
    1

    2

    3

    4

    5

    6

    7

    8

    9

   10

   11
              Iowa

              Michigan

              Wisconsin

              Michigan

              Ohio

              Michigan

              Michigan

              Oklahoma

              Michigan

              Michigan

              Arkansas
Jan '76
Jan '76
Jan '76
Jan '76
Feb '76
Jan '77
Jan '76
Jan '76
Sept '76
Dec '76
Jan '77
- Dec '76
- Dec '76
- Dec '76
- Dec '76
- July '77
- Dec '77
- Jan '77
- Dec '76
- Aug '77
- Nov '77
- Dec '77
2-stage

1 -stage

2-stage

1 -stage

1 -stage

2-stage

1 -stage

2-stage

1 -stage

1 -stage

2-stage
   33.7

    0.5

    7.6

    0.8

   15.8

    0.9

   11.6

   12.3

    4.0

    0.8

    6.0

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running averages had to be determined
in the same manner as the distributions
of daily averages.
Summary Statistics
  The  following  summary  statistics
have been computed for daily,  7-day
average,  and  30-day average effluent
BODs and SS concentration data from
each plant:

     arithmetic mean
     standard deviation
     coefficient of variation
     skew coefficient
     maximum observation
     minimum observation
     number of valid observations

  Examination of these statistics  will
provide  information on  the central
tendencies, ranges and shapes  of the
effluent  BOD5 and SS concentration
distributions for data from each plant.
Theoretical Distribution
Models
  Virtually  an  unlimited  number of
theoretical distribution functions could
be tested for fit with the effluent data.
Practical limitations prevent  testing
more than  a  few.  The normal,  log-
normal, and gamma distributions are
good candidates because of their sim-
plicity, widespread  use, and demon-
strated usefulness in previous studies
of   wastewater  treatment  process
performance.
Empirical Distribution Models
  Strong linear relationships between
annual mean and percentile concentra-
tion values have been shown to exist in
previous studies.  For  example, if one
year's effluent BOD5 data for a group of
plants is placed in ascending order, the
95th  percentile value  will be approxi-
mately  proportional   to the  plant's
annual mean value.
  The set of linear regression equations
developed for the percentile values do
not, by themselves, define a continuous
probability distribution. However, given
the mean value from  a  set of data, a
finite number of  predicted percentile
values can be determined. A continuous
distribution can then be constructed by
linearly interpolating between each of
the predicted percentile values.
Kolmogorov-Smirnov
Goodness-of-Fit Test
  The usefulness of each of the five
(proposed)  distribution  functions  for
modeling BODS and SS data from each
plant  was  examined using the
Kol mogorov-Smi rnov  goodness-of-f it
test. The null hypothesis  for this test is:

  "The observed data were sampled
  from  an  underlying  population
  which is distributed according to
  the   hypothesized   distribution
  function."

It is assumed that if the test hypothesis
is  true,  any differences between  the
observed distribution and the hypothe-
sized distribution are due to the effects
of random sampling.
Randomness of Effluent Data
  It was important to determine if trick-
ling filter effluent BODs and SS concen-
tration data were random. If the trickling
filter effluent data are not random, then
distribution models for effluent data are
not random, and distribution models for
effluent concentrations cannot be used
in  the  classical  manner  because
probability density functions are usually
assumed to be  based  on random data.
Another  consequence of nonrandom-
•ess would be  that predictable cycles
might exist  in effluent data.
  The  randomness of  trickling filter
effluent  BOD5  and SS  concentration
data have been tested using the runs
test and a test for serial correlation. In
addition  to  simply determining if the
data are random,  these tests can be
used to determine the relative magni-
tude of nonrandomness in each set of
data and to provide a basis for compar-
ing the relative randomness of different
sets of data.
  The runs test  is a general purpose test
of the randomness of  a data  series.
Trend, grouping,  and many types of
cyclical movement in  the data can be
detected. In some cases, the runs test
fails to detect dependence between suc-
cessive values  of  a data series.  It  is
therefore prudent to perform a test for
correlation between consecutive  data
pairs (serial correlation) in  addition to
the  runs test  when  examining  data
randomness.
  The  test  for serial correlation is per-
formed by  treating the original  data
series  as one variable, xt, and treating
the  data  series shifted forward  one
observation as the other variable, xt-i.
The Pearson  correlation coefficient, a
measure of association of the strength
of the linear relationship between two
variables, is then computed to test for
correlation  between xt and xt-i. If the
data are random, the correlation coeffi-
cient will tend towards zero.
Annual and Weekly Cycles
  To the test for the possibi lity of weekly
or annual cycles in trickling filter efflu-
ent data, a two-way analysisof variance
was performed on effluent BODs and SS
concentration  data from  each  plant.
Analysis of  variance was not  used to
explicitly determine if cycles existed in
the data series, but merely to determine
if effluent concentrations were depend-
ent on the month or day of the week on
which they occurred. However, if efflu-
ent BOD5 (or SS) concentrations from a
treatment  plant  were  found  to  be
dependent on the day of the week, it was
concluded that a  weekly cycle existed in
that data set.
  A  similar  conclusion  concerning
annual  cycles could not  be made  for
data that were found to be dependent on
the month. With only 1 year of data from
each plant, there was no way of deter-  i
mining if an observed annual sequence
was  representative of  a  repeating
annual cycle or merely part of a random
fluctuation in average monthly perform-
ance. All that could be concluded in this
case was that some variation in the data
was  due to  significant  changes in
average  performance from  month to
month.


Effects of Various Process
Parameters
  The  effects  of  influent,  environ-
mental, and operational parameters on
effluent variability  were  examined by
using  multiple  linear  regression
analysis. This  analysis studied  the cor-
relations  between  various  process
parameters and  effluent BOD5 and SS
concentrations.
  The regression  analysis  has been
conducted  in  two parts. First,  multiple
linear regression equations predicting
effluent BOD5 and SS concentrations
for each plant were constructed using
linear combinations of the following
independent variables:

    flow, Q
    influent BODs and SS concentra-
     tions, BOD,, SS,                M

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     primary  effluent BOD5  and  SS
      concentrations, BODP, SSP
     the inverse of the average influent
      water temperature, WTEMP"1
     the inverse of the average air tem-
      perature, ATEMP'1
     the deviation from 7.0 of the influ-
      ent pH, 7.0-pH
     recycle ratio, R

   Second, to test the relation  impor-
 tance of  quadratic terms,  loading
 factors,  combinations of  terms and
 values  of parameters from  previous
 days  (lagged  variables),  regression
 equations using the terms above as well
 as the following terms were constructed:

     quadratic  terms:  BOD,2,   SS,2,
      BODp2,  SSPZ

     loading  factors: Q-BOD,,  OSS,,
      Q-BODp, Q-SSp

     combinations of  terms:  T»va  =
      (ATEMP +  WTEMP)/2,   Td  =
      | ATEMP-WTEMP |

     lagged   terms:   BODi,t-i,  SSi,t-i,
      BODp,t-i, SSp,t-i, BODi,t-2,  SSi,t-2,
      BODp,t-2, SSp,t-2, ATEMPt-i


 Measurement Error
   Any discussion of the causes of varia-
 tion in a series of measured quantities
 should  include  consideration  of the
 effect of  measurement error. Like all
 laboratory procedures, the BODs  and SS
 tests have a  finite degree of precision.
 Thus even if the effluent from a treat-
 ment plant was of  perfectly uniform
 quality at all times, and in addition, the
 effluent  was always sampled without
 error, the measured  BODs and SS con-
 centrations would still vary from sample
 to sample because of the inherent vari-
 ability in the methods of analysis.
   It is possible to determine how much
 variation  in trickling  filter effluent data
 is  caused  by  measurement  errors
 because the variation due to the  limited
 precision of the BOD5 and SS tests is
 independent of other sources of varia-
 tion in the data.
   A simplifying  assumption has been
 made to estimate the variation  due to
 measurement error.  Because the preci-
 sion of  the  BODs and SS tests is a
 function  of the concentrations being
 measured, the precision of these tests
 varies from day to day. For simplicity, it
 wilt  be  assumed that  the fractional
.standard  deviations  of the measure-
ment  errors are  a  function of each
plant's annual mean effluent BODs and
SS concentrations.
Results
  The characterization of daily, running
7-day average and running 30-day aver-
age effluent BODs and SS concentration
data was conducted in three steps. First,
summary  statistics  for  each type of
effluent data set were  computed for
each treatment plant (Tables 2, 3,  and
4). Second, coefficients for two empiri-
cal distribution models were computed.
Third, two empirical and three theoreti-
cal  distribution models were tested to
determine their usefulness in modeling
effluent data from each plant.
  Some general characteristics of trick-
ling filter effluent data can be seen from
these results.  For the daily data,  the
averages of the mean BODs from all
plants and the mean SS  concentration
from all plants are approximately equal.
However, the average standard devia-
tion for SS data is somewhat higher
than the average standard deviation for
BODs data. Thus it may be concluded
that, overall, BOD5 and SS data tend to
be of the same average magnitude but
that SS data tend to be somewhat more
variable.
Comparison of Distribution
Models
  Five probability  distributions have
been tested for their fit to effluent BODs
and  SS  data  using the  Kolmogorov-
Smirnov  (K-S)  goodness-of-fit  test.
Distributions tested were the one- and
two-parameter  empirical models and
the normal, log-normal,  and gamma
distribution functions.
  For any  single effluent  parameter
(e.g., daily BODs, 7-day average SS, etc.)
the distribution that best fit the data
changed  from treatment plant to treat-
ment  plant. However, it  can be con-
cluded that, in general, the log-normal,
gamma,  and two-parameter empirical
distribution functions are equivalent for
the purpose of describing effluent data
distributions.
Randomness of Effluent Data
  Using a 5-percent significance level
as the rejection criterion, the hypothesis
of randomness can be rejected for every
set of BOD and SS data on the basis of at
least  one of the two tests.  It can be
concluded that the BOD data from every
plant and the SS data from every plant
but one are highly nonrandom. For 9 out
of the 11  treatment plants,  it  can  be
concluded that, for most of the treat-
ment plants studied, effluent BODs data
are less random than SS data.
Annual and Weekly Cycles
  It may be concluded from the results
of the randomness tests that there are
significant nonrandom patterns in trick-
ling filter effluent data. The existence of
annual or weekly cycles in the data has
been examined by performing a two-
way analysis of variance on BODs and
SS data from each plant and by pooling
normalized data from all the treatment
plants  and  plotting the  averages  for
each month and day of the week.
  For each treatment plant studied,
effluent BODs and SS concentrations
are in some manner dependent on the
month in which they occur. The ratios of
maximum monthly average to minimum
monthly average  and the differences
between  monthly  averages are  of
physical as well  as  of  statistical
significance. Because only 1 year of
data from each plant was used,  it may
not be concluded that effluent concen-
trations at  these  11  treatment  plants
have repeating annual cycles.
  The effluent concentrations in eight
sets of  data depend on the day of the
week on which they occur. Because
there are 52 weeks in each set of data, it
may be concluded that there is a repeat-
ing weekly  cycle in each of these eight
data sets.  Existence of any general
weekly or annual patterns in the efflu-
ent data from all the treatment plants
was studied by plotting  monthly  and
daily averages for pooled data. Effluent
BOD5 and SS concentration data from
each treatment plant were first normal-
ized. Normalized  data from  all  of the
treatment plants were pooled together
and grouped according to the month in
which the  data were taken. To study
weekly patterns, effluent data from the
nine treatment plants with daily data
were pooled and then grouped accord-
ing to the day of the week on which the
data occurred. Effluent  BODs and  SS
data tend to have annual cycles that are
roughly in  phase with one another.
Concentrations of both  BODs and  SS
tend to  be  higher than average in the
winter months and lower than average
in the summer and early fall.

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Table 2.
Summary Statistics for Daily Effluent BODs and Suspended Solids Concentrations
BODs
Plant
Number
1
2
3
4
5
6
7
8
9
10
11
Average
Median

Mean
(g/m3)
33.31
10.73
10.05
58.35
29.24
27.04
23.21
43.09
51.13
21.02
18.31
29.59
-
Sx
(g/m3)
15.28
7.24
7.52
20.83
11.21
6.75
16.93
6.59
19.95
6.22
11.98
11.86
11.21
V,
0.46
0.67
0.75
0.36
0.38
0.25
0.73
0.15
0.39
0.30
0.65
0.46
0.39
Skew
0.92
1.62
1.68
0.10
1.31
0.09
1.62
0.61
0.23
-0.15
2.17
0.93
0.92
Max
102.0
43.0
61.0
107.0
93.0
58.0
100.0
69.0
125.0
33.0
104.0
-
93.0
Min
(g/m3)
8.0
0.0
1.0
22.0
6.0
3.0
1.0
28.0
12.0
8.0
0.0
-
6.0
Valid
Obs
357
365
362
250
492
365
383
365
359
250
355
-
-
Mean
(g/m3)
52.50
21.45
21.04
54.85
18.34
15.09
24.05
34.03
41.08
23.60
16.15
29.29
-
Sx
(g/m3)
31.17
13.35
16.69
15.02
8.81
5.98
15.09
13.63
17.61
14.42
8.85
14.60
14.42
Suspended Solids
Vx
0.53
0.62
0.79
0.27
0.48
0.40
0.63
0.40
0.43
0.61
0.55
0.52
0.55
Skew
2.26
1.15
1.17
0.10
2.16
0.40
0.99
4.56
1.60
1.08
1.09
1.51
1.15
Max
(g/m3)
280.0
80.0
88.0
94.0
84.0
37.0
86.0
172.0
130.0
86.0
64.0
--
86.0
Min
(g/m3)
6.0
1.0
1.0
20.0
3.0
2.0
2.0
12.0
12.0
2.0
1.0
-
2.0
Valid
Obs
356
366
361
250
493
365
386
365
365
250
363
--
--
Effects of Process Parameters
  Correlations between influent, opera-
tional,  and   environmental  process
parameters and effluent  BOD and SS
concentrations  were  studied  using
multiple  linear regression  analysis.
Regression equations using  combina-
tions of various process parameters as
independent variables were  construc-
ted in a stepwise manner. All data used
in   constructing  the  regression
equations were normalized so that the
regression coefficients would be easier
to interpret.
  The  regression  analysis  was
conducted in two parts. First, regression
equations were developed using simple
linear combinations of untransformed
variables. In the second  part,  various
transformations and combinations of
the independent variables used in the
first part were included in the  regres-
sion analysis. In both steps,  only those
independent  parameters  that  contri-
buted materially to the significance of
the regression equation were included
in the final form of the equation.
  It was concluded that primary effluent
BODs  concentration  is,  in general,
highly  correlated with  effluent BOD5
concentration. Flow rate, influent BODs
concentration, and  the reciprocal of
influent wastewater temperature are
also highly correlated with the effluent
BOD5 for most plants where data were
available. Results for the other indepen-
dent  parameters  are  inconclusive,
because there was little correlation, the
correlation varied from plant to plant, or
because data for that variable were not
available from most plants. The regres-
sion equations for effluent SS generally
have somewhat lower coefficients of
determination, indicating that effluent
SS concentrations are not correlated to
other process parameters as highly as
are effluent BOD5 concentrations.


Variation Caused by
Measurement Error
  The estimates of variation caused by
measurement error are given in Table 5
for  effluent concentration data from 11
trickling filter plants. Variation caused
by the SS test  is greater than for the
BODs test for every plant. The standard
deviation of the analytical tests is esti-
mated to be between 1.8 and 7.5 g/m3
for  BODs  and between 5.3 and 10.7,

-------
Table 3.   Summary Statistics for Continuous 7-Day Averages Effluent BOD and Suspended Solids Concentrations

                         BODS                                                     Suspended Solids
Plant
Number
1
2
3
4
5
6
7
8
9
10
11
Average
Median
Mean
(g/m3)
33.12
10.56
9.99
58.31
29.15
27.02
22.87
43.00
51.49
21.01
17.99
-
—
Sx
(g/m3)
11.52
4.95
4.76
20.17
6.86
3.86
13.32
4.79
17.47
4.59
8.40
9.15
6.86
V,
0.35
0.47
0.48
0.35
0.24
0.14
0.58
0.11
0.34
0.22
0.47
0.34
0.35
Skew
0.15
0.93
0.67
0.07
0.57
-0.39
1.48
0.45
-0.07
0.21
1.28
0.49
0.45
Max
(g/m3)
62.3
25.4
24.6
96.8
53.1
35.7
81.7
54.3
82.9
29.6
48.7
--
53.1
Min
(g/m3)
14.0
3.1
1.7
27.2
14.0
14.4
7.1
34.1
18.3
10.0
4.5
-
14.0
Valid
06s
360
360
360
360
514
359
391
360
359
359
358
-
--
Mean
(g/m3)
52.52
21.22
21.14
54.82
18.41
15.07
23.62
34.04
41.23
23.86
16.11
-
-.
Sx
(g/m3)
18.63
9.18
8.00
12.65
5.26
4.40
9.84
7.17
12.57
9.59
5.07
9.31
9.18
Vx
0.35
0.43
0.38
0.23
0.29
0.29
0.42
0.21
0.30
0.40
0.30
0.33
0.31
Skew
0.38
0.70
0.26
-0.03
0.73
0.04
0.59
1.61
0.73
1.21
0.60
0.62
0.60
Max
(9/m3)
103.6
49.1
41.5
78.8
39.0
27.4
52.3
63.1
80.3
59.2
34.7
--
52.3
Min
(9/m3)
17.6
6.1
5.1
30.0
7.3
6.4
5.9
24.1
18.3
6.4
5.9
-
6.4
Valid
Obs
360
360
360
360
516
359
391
360
359
'359
359
-
--
g/m3 for SS. Although these results are
only approximate, it is apparent that a
significant percentage of the variation
in effluent concentration data is due to
the limited precision of the BOD5and SS
tests.

Discussion
  For any given parameter, the distribu-
tion that was best for describing effluent
data was  generally different for each
treatment plant. Three distributions (the
two-parameter empirical,  log-normal,
and gamma) were generally comparable
and could be used to describe effluent
data better than the other two distribu-
tions tested.  These three distributions
can be used to model  effluent distribu-
tions  adequately,  but  no  single
distribution, empirical  or theoretical, is
generally  best for  modeling  effluent
distribution  from  all   trickling filter
plants. The general characteristics of
trickling  filter  effluent concentration
distributions found in  this study are in
agreement with the results of previous
studies on trickling filter and activated
iludge plants.
  All of the distributions but the one-
parameter model fit trickling filter efflu-
ent data with roughtly the same degree
of accuracy.  Therefore, the choice of
distribution  to  use in any  particular
application can be made on the basis of
the relative ease with which the differ-
ent distributions are  calculated. Even
the one-parameter model can be used to
obtain a reasonable, rough estimate of
effluent  distributions and   may be
preferable in some applications.
  The  advantage  of  using  the  one-
parameter empirical model is that the
distribution standard deviation does not
have  to  be  calculated or estimated.
When the standard deviation must be
estimated, the one-parameter model is
as accurate for predicting effluent distri-
butions as any of the two-parameter
models,  unless an   unusually  good
estimate of  the standard  deviation is
available.
  Percentiles can be  calculated easily
for any of the five distributions once the
parameters   of   the   distribution  are
specified.  The  arithmetic mean and
(when required) the standard deviation
are sufficient to specify any of the distri-
butions except the log-normal. To use
the log-normal distribution, the mean
and  standard  deviation  of   the
logarithms of the data are required, and
these are not the same as the loga-
rithms  of  the arithmetic  mean  and
standard deviation.
  If  a  desired  percentile value is
specified  and (when  required)  the
standard deviation is estimated, any of
the five distributions can be  used to
compute the implied mean value of the
distribution. If the gamma distribution is
used, an iterative procedure (which is
time-consuming unless performed on a
computer) must be used to calculate the
distribution  mean.
  Another  disadvantage of  using  the
gamma distribution is that  only rela-
tively  incomplete tables of  gamma
distribution  values are available,  and
gamma  percentiles   are  difficult  to
compute. In contrast, complete tables of
the normal  distribution are available.
With some simple conversions, normal
tables can be used for the log-normal
distribution  as well. Of course, tables

-------
Table 4.  Summary Statistics for Running 30-Day Average Effluent BOD and Suspended Solids Concentrations

                                BOD5                                         Suspended Solids
Plant
Number
1
2
3
4
5
6
7
8
9
10
11
Average
Median
Mean
(g/m3)
32.11
10.00
9.77
57.69
29.03
26.78
22.59
42.75
52.94
21.10
17.05
--
-
Sx
(g/m3)
9.91
3.53
3.69
19.85
5.16
2.39
11.63
3.98
15.56
3.48
6.18
7.76
5.16
V»
0.31
0.35
0.38
0.34
0.18
0.09
0.51
0.09
0.29
0.16
0.36
0.28
0.31
Skew
0.15
1.04
0.77
0.11
0.57
-0.74
1.09
0.57
-0.16
0.66
0.84
0.45
0.57
Max
(g/m3)
51.9
20.7
19.6
88.9
42.7
31.0
52.1
51.7
79.0
27.9
34.5
--
42.7
Min
(3/m3)
17.0
5.4
4.2
32.0
20.0
20.2
10.1
36.8
25.1
15.7
7.8
--
17.0
Valid
Obs
337
337
337
337
516
336
368
337
336
336
336
-
-
Mean
(g/m3)
51.59
20.93
21.05
54.30
18.58
14.85
23.22
34.01
41.81
23.99
15.81
-
--
Sx
(g/m3)
15.63
7.66
5.47
11.54
3.81
3.88
7.55
4.50
9.79
6.25
3.44
7.23
6.25
V.
0.30
0.37
0.26
0.21
0.21
0.26
0.33
0.13
0.23
0.26
0.22
0.25
0.26
Skew
0.78
0.93
0.09
-0.02
0.27
-0.05
0.16
'0.07
0.40
0.23
'0.06
0.24
0.16
Max
(g/m3)
88.9
41.1
31.5
72.2
30.5
21.1
38.6
43.1
61.7
39.4
24.9
-
39.4
Min
(g/m3)
29.7
9.5
11.0
37.3
11.2
7.47
11.2
26.0
26.8
11.3
7.9
-
11.2
Valid
Obs
337
337
337
337
516
336
368
337
336
336
336
-
--
are not available for the two empirical
distribution models, but the calculations
involved in using these distributions are
simple.

Effluent Standards
  The distributions of effluent concen-
trations must be known if reasonable
and consistent effluent standards are to
be set. A reasonable standard does not
require a level of treatment that cannot
be achieved with available technology.
Two standards are consistent if neither
standard requires a significantly higher
level of treatment than  the other.
  Of the 11  treatment plants in this
study, two  had  a mean effluent BOD5
concentration equal to or less than 17.5
g/m3 and three  had a mean effluent SS
concentration equal to or less than 18.6
g/m3.  U.S.  Environmental Protection
Agency  (EPA)  secondary  standards
were attained by a relative minority of
trickling filter plants  evaluated in this
study.
  The  implied  mean effluent  BOD5
concentration for the 7-day  average
standard is 4.8 g/m3 higher than the
implied mean for the 30-day standard.
For effluent  SS  concentrations,  the
implied mean for the 7-day standard is
2.5 g/m3 higher than for  the 30-day
standard.  From  these  results,  the
conclusion may be made that the 30-
day effluent  BOD5 standard is signifi-
cantly more  restrictive than the  7-day
standard, and that the 30-day effluent
SS standard  is somewhat more restric-
tive than the 7-day  SS standard. EPA
secondary standards are therefore not
consistent when  applied  to trickling
filter treatment plants.
Randomness of Effluent Data
  Effluent concentration data in this
study are not randomly generated and
are thus not independent of time and
preceding effluent concentrations. The
probability that an effluent BOOs (or SS)
concentration will be exceeded on any
one day is dependent on which particu-
lar  day  is  of  interest.  Information
concerning the conditions under which
events occur is not included in distribu-
tion functions, and hence no statement
concerning  the  probable  level  of
effluent concentrations on any one day
can be made based simply on the distri-
bution models considered in this study.
  Distribution   models  for  effluent
concentrations do provide  information
on the long-term behavior of trickling
filter performance and can be used to
estimate the  probable  frequency of
occurrence of some  range of effluent
values over a 1-year period.

Annual and Weekly Patterns
  On  the  basis  of  the analysis of
variance results and the plots of pooled,
normalized  data,  the  following
conclusions  can be made concerning
effluent BOD5 and SS concentrations at
the 11 treatment plants studied.     ^
                                  8

-------
   1.  There  are  significant month-to-
      month  fluctuations  in  average
      effluent concentrations for every
      treatment plant studied.

   2.  One cause of these monthly fluc-
      tuations is  an annual cycle in
      effluent  concentrations that is
      evident when data  from all  11
      treatment plants is pooled. Efflu-
      ent  concentrations  tend to  be
      above  average in the winter and
      below  average in the summer.

   3.  A significant weekly cycle exists in
      effluent concentration data  from
      some  of the treatment plants
      studied.

   4.  When  data from nine treatment
      plants  were pooled, weekly cycles
      were evident  for BOD5 and SS
      data, but the amplitudes of these
      cycles  were  less than  half the
      amplitudes  of the annual cycles.
      Effluent BOD and SS concentra-
      tions tend to be below average on
      Sundays, and  SS concentrations
      tend to be above  average  on
      Wednesdays.
 Effects of Process Variables on
 Effluent Concentrations
   Based on the results of the multiple
 regression  analyses,  it can  be con-
 cluded that  influent, operational, and
 environmental process  variables  are
 correlated with effluent BOD5 and SS
 concentration  data at trickling filter
 plants.  It  has not  been proven that
 fluctuations in effluent data are caused
 by changes in  the independent param-
 eters because regression analysis alone
 cannot  be  used to prove cause and
 effect  relationships.  Correlations
 between two  variables  conceivably
 could be caused by a third, unmeasured
 variable. However, the relationships
 found are consistent with current theo-
 retical models of clarifiers and trickling
 filters and provide strong  evidence to
 support the hypothesis that a significant
 percentage  of effluent  variation  in
 trickling filter plants is caused by fluctu-
 ations  in  process  loading  and
 temperature.
 Variation Due to Measurement
 Error
   Measurement  errors can  cause a
.significant  percentage of  the total
 Table 5.  Variation Caused by Measurement Error

            Effluent BODS
      Effluent Suspended Solids
           Estimated Percent    Standard    Estimated Percent    Standard
  Plant    of Variation Caused   Deviation    of Variation Caused    Deviation
 Number by Measurement Error   (g/m3)   by Measurement Error    (g/m3)
1
2
3
4
5
6
7
8
9
10
11
10
7
6
13
15
36
5
78
12
28
6
4.7
1.9
1.8
7.5
4.4
4.0
3.6
5.8
6.9
3.3
3.0
11
23
14
51
44
78
21
37
27
15
37
10.5
6.4
6.3
10.7
5.9
5.3
6.8
8.2
9.1
5.5
5.4
variation  in  effluent concentrations,
especially for SS data. The percentage
of the variation in effluent data caused
by analytical error was estimated to be
greater than  10 percent for BOD5 and
SS concentrations from 7 and 11 plants,
respectively, and was greater than 20
percent for BOD5 and SS data from 3
and 8 plants, respectively.


General Comments
  The greater variability and random-
ness found in SS effluent data may, in
part, be due to the fact that measure-
ment  errors appear to be somewhat
greater for SS than for BOD5. However,
one reason that measurement errors
were estimated to be greater for SS data
was  because of the  assumption that
duplicate BOD5 samples were analyzed,
but only one SS sample was used. If this
assu mption is not correct, the esti mated
measurement  errors for  SS analysis
would be smaller, though they would
still be somewhat  larger than the esti-
mated errors for BODS data.
  The  sum  of the  percentages  of
variance explained by the regression
models  and by  measurement error is
greater than 40 percent for all but three
sets of data. From these results, it can
be  concluded that a large fraction of
effl uent variability iscaused by variation
in process parameters and by measure-
ment error. Because three of the sums
are greater than  100 percent,  the
estimates of measurement error are in
some cases too high.
  Measurement errors do not affect the
values of running average data as much
as they affect daily data, because the
errors tend to cancel out. For example, if
the standard deviation  of a test is 5.0
g/m3, the standard deviations of 7-day
and  30-day averages  for  the  same
parameter would be 1.9 and 0.9 g/m3,
respectively.
  Based on the results  of this study, it
may be concluded that the magnitude of
variation in effluent data caused by
measurement errors  is quite large at
many treatment plants. If a treatment
plant is  operating  near its discharge
limits, apparent violations of the daily
and 7-day average concentration limits
can be caused by analytical error alone.
  To reduce the possibility of discharge
violations  caused  by   measurement
error, more replicates of each sample
can be analyzed.  Also, sampling and
laboratory techniques  should be

-------
checked to see that all sources of samp-
ling and analytical errors are minimized.


Reliability of Trickling
Filter Process
  The  uncertainties  associated  with
plant  design and operation should be
recognized in the design  of trickling
filters. The best way to accomplish this
is  to  incorporate  stochastic concepts
and procedures in the design process.
To produce  an effluent of high quality
and  to  meet the  effluent discharge
requirements at minimum cost, design
engineers must be able to estimate the
average effluent quality and its varia-
tions  for  a  given treatment process.
Uncertainties and their significance on
process performance can be analyzed
systematically using methods of proba-
bility.  A  probabilistic approach  for
design can be used to provide a consis-
tent basis for the analysis of uncertainty
and a theoretical basis for the analysis
of performance and reliability.
  Reliability measures are expressed in
probability terms and are defined as the
probability of adequate  performance
(i.e., the percent of the timethat effluent
concentrations meet  requirements). A
stochastic model and design tables and
graphs have been developed in a related
study  predicting   achievable  effluent
BODs and SS concentrations based on a
proposed  coefficient of  reliability
(COR).* The  COR relates mean constitu-
ent  values  to the  standard  and is
achieved on a probability basis and the
log-normal  distribution  assumption.
The COR was defined mathematically
as:

COR =
 (Vx2 +  1 )1'2 exp { -Z ,.„ [1 n (V«2 +  1 )]1/ZJ

  The coefficient  of variation (Vx)  for
  effluent concentration  should  be
  estimated  from  past  experience
  and on reasonable expectation  of
  performance. Values of the coeffi-
  cient  of variation  (Vx) from other
  similar  treatment plants may  be
  used as a guidance. Based on cur-
  sory  examination,   coefficient   of
  variations of 0.50  for  BODs and
  0.55  for SS concentration would
  appear to  be suitable values,  how-
                                           ever. Reliability as a function of Vx
                                           and COR is shown in Figure 1.
                                           To support the validity of the reliability
                                         model in prediction of performance of
                                         trickling filters, the percent of the time
                                         that effluent concentration exceeded 30
                                         g/m3 was computed for 1 year of data in
                                         all 11 plants (Table 18 in the full report).
                                         This percent of exceedance  was also
                                         predicted using the  reliability  model
                                         based on the log-normal assumption.
                                         The  prediction  values   are  very
                                         comparable with the measured values.
                                           To determine  the  validity  of  the
                                         theoretical results of the COR, Figure 1
                                         was reconstructed based on the 1 year
                                         of  operational data of  all 11  plants.
                                         Percentile values  of  exceedance for
                                         effluent BODS and SS from one year of
                                         daily operational data have been com-
                                         puted to present an empirical distribu-
                                         tion of effluent concentration without
                                         considering  whether it follows  any
                                         classical  distribution  function.   The
                                         results of COR  based on pooled plant
                                         data  were  highly comparable to the
                                            1.0
                                            0.9
                                            0.8  -
                                            0.7
                                            0.6
                                          •§0.5

                                          1
                                            0.4
                                            0.3
                                            0.2
                                            0.1
*Niku, S.,E D.Schroeder, G Tchobanoglous, andF.
 J  Samaniego, "Performance of Activated Sludge
 Plants  Reliability,  Stability and Variability,"
 EPA-600/2-81-227, U.S  Environmental Protec-
 tion Agency, Cincinnati, OH, September 1981.
                                               0.1  0.2  0.3  0.4  0.5  0.6  0.7  0.8  0.9   1.0  1.1   1.2   1.3   1.4   1.5

                                                                        Normalized Mean, m^/Xt
Figure 1.    Reliability  versus normalized mean  for different  coefficients  of
             variations.                                                        *
                                  10

-------
results of COR based on the log-normal
distribution assumption.

Stability of Trickling Filter
Plants
  Stability is a measure of adherence of
the annual  mean  concentration,  and
"standard deviation" is the most appro-
priate  measure of stability in waste-
water treatment plants.
  In  Figures  2  and 3,  the mean,
standard deviation, and range of efflu-
ent BOD5 and SS data for all 11 trickling
filter plants are shown. Examination of
these descriptive statistics results in the
conclusion that a stability cut-off point
of 10 g/m3  can  be used. That is, a
distinct difference  exists between the
statistical characteristics of the plants
operating below and above the standard
deviation of  10 g/m3. The maximum
daily  concentrations of effluent BODs
and SS in stable plants with a standard
deviation of less than or equal to 10
g/m3 are  usually less than 70 g/m3;
whereas in unstable plants, maximum
concentrations are usually greater than
90 g/m3 and 80 g/m3, respectively.
  The full  report  was  submitted in
fulfillment of Grant No. R805097-01 by
the University of California under spon-
sorship of  the  U.S.  Environmental
Protection Agency.
I

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                                                                        Standard Deviation (g/m3)
                                                             20
                                                                       24
                                        Figure 2.    Variability of effluent BOD as a function of standard deviation.
                                                                                11

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Figure 3.    Variability of effluent suspended solids concentration as a function of
             standard deviation.
                                  12

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Richard Haugh, Edward D. Schroeder, and George Tchobanoglous are with the
  University of California. Davis, CA 95616; Salar Niku is presently with Brown
  and Caldwell Consulting Engineers. Pasadena, CA 91109.
Jon Bender is the EPA Project Officer (see below).
The complete report, entitled "Performance of Trickling Filter Plants: Reliability,
  Stability and Variability," (Order No. PB 82-108 143; Cost: $ 11.00. subject to
  change) will be available only from:
        National Technical Information Service
        5285 Port Royal Road
        Springfield, VA 22161
        Telephone:  703-487-4650
The EPA Project Officer can be contacted at:
        Municipal Environmental Research Laboratory
        U.S. Environmental Protection Agency
        Cincinnati, OH 45268
                                                                              13
                                                                         •ft U.S. GOVERNMENT PRINTING OFFICE:1981--559-092/3355

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