United States
                   Environmental Protection
                   Agency
 Hazardous Waste Engineering
 Research Laboratory
 Cincinnati OH 45268
                   Research and Development
 EPA/600/S2-86/076 Feb. 1987
v>EPA         Project  Summary
                   Soil,  Clay,  and  Caustic Soda
                   Effects  on  Solubility,  Sorption,
                   and  Mobility of
                   Hexachlorocyclopentadiene
                   S. F. Joseph Chou and Robert A. Griffin
                    This study on the aqueous chemistry,
                   sorption, and mobility of hexachlorocy-
                   clopentadiene  (C-56) in soil materials
                   was initiated to provide information on
                   its behavior in the environment, partic-
                   ularly on its movement through soil at
                   land disposal facilities for hazardous
                   wastes. Studies showed that the solu-
                   bility of C-56 in water, soil extracts, and
                   sanitary landfill leachates ranged from
                   1.03 to 1.25 ppm. Sodium hydroxide
                   (caustic soda) and sodium chloride de-
                   creased the solubility of C-56 in water
                   as the salt concentration increased;
                   sodium hypochlorite slightly increased
                   the solubility of C-56; and sodium
                   perchlorate had no significant effects
                   due to increasing salt concentration on
                   the solubility of C-56. A caustic brine
                   composed of a mixture of the salts was
                   intermediate in decreasing solubility.
                    The half-life of C-56 in water was
                   about three months at both 22°C and
                   35°C, indicating little temperature de-
                   pendence. C-56 is very photosensitive,
                   and its half-life was less than four min-
                   utes in aqueous solution and less than
                   1.6 minutes in hexane or methanol so-
                   lution.  Studies also indicated that pH
                   did not significantly affect the C-56 hy-
                   drolysis rate in aqueous solution; how-
                   ever, iron caused an increase in the hy-
                   drolysis rate. At least 12 products of
                   photolysis and hydrolysis were
                   identified. Pentachlorocyclopentenone,
                   hexachlorocyclopentenone,  pen-
                   tachloropentadienoic acid, cis- and
                   transpentachlorobutadiene, and tetra-
                   chlorobutenyne were the major  prod-
                   ucts identified.
  The presence of salts in solution dra-
matically affected the sorption of C-56;
brine, NaCI, and NaOH caused  an in-
crease in sorption while NaOCI caused
a decrease in sorption. There was also a
high direct correlation between the
total organic carbon (TOO content of
soils and the amount sorbed.
  C-56 remained immobile in the soils
when leached with water, landfill
leachate, or caustic brine but was
highly mobile when leached with or-
ganic solvents. In a soil column  leach-
ing study, some hydrolysis products of
C-56 were much more mobile than
C-56, indicating they might migrate and
generate environmental problems.
  This Project Summary was  devel-
oped by EPA's Hazardous Waste Engi-
neering Research Laboratory, Cincin-
nati, OH, to announce  key findings of
the research project that is fully docu-
mented in a separate report of the same
title (see Project Report ordering infor-
mation at back).

Introduction
  Environmental concerns have created
a demand for information on land dis-
posal of toxic organic substances such
as hexachlorocyclopentadiene (C-56 or
"hex" waste). C-56 is a highly toxic
compound that produces systemics of
unknown mechanism in mammals and
has  been reported to have caused sig-
nificant decreases in survival of fathead
minnows at concentrations as low  as
7.3 ppb.
  C-56 has no end uses of its own but is
commercially important as a chemical

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intermediate for producing chemical
feed stocks, pesticides, adhesives,
resins, and other related products. The
present environmental concern is  the
disposal of large quantities of industrial
wastes containing C-56. A major source
of C-56 wastes comes from manufactur-
ing chlorinated insecticides.
  The  phenomenon of salting-out  a
nonelectrolyte from an aqueous solu-
tion by adding salt is well known. Thus,
the high salt content of the caustic soda
brine waste water would be expected to
cause changes in the aqueous solubility
of C-56. Because sorption of C-56 by soil
materials is a function of concentration,
knowledge of how the solubility
changes with brine concentration is im-
portant to accurately predict attenua-
tion and mobility.
  C-56 is  extremely volatile and pho-
toreactive in sunlight. Near-surface half-
lives of C-56 in aquatic systems are  less
than 10 minutes. C-56 shows an unex-
pected tendency to undergo the Diels-
Alder reaction with many dienophiles at
temperatures between 20°C and 200°C.
It condenses even with simple olefins
which normally do not react with dienes
and with  polynuclear aromatic hydro-
carbons. Thus, the apparent disappear-
ance of C-56 from the  environment
should not be construed to mean that it
is  always degraded to smaller
molecules; it may condense  into larger
molecular weight compounds.  Al-
though C-56 has been used in the chem-
ical industry for decades, there  is a
dearth of information on the environ-
mental impacts of the compound on
aquatic or soil systems.
  The principal objectives of this re-
search were to determine the attenua-
tion mechanisms  and capacity of se-
lected clay minerals and  soils for C-56,
to determine the effects of caustic brine
on the attenuation and solubility of
C-56, to study the aqueous chemistry of
C-56, and to develop a chemical model
to  predict C-56  migration through  soil
materials.

Experimental Materials
  A reagent grade C-56  was obtained
from Pfaltz and Bauer, Inc., Stamford,
CT, and was used without further purifi-
cation. An analytical standard  of C-56
(lot #0213) was also obtained from  EPA
in Research Triangle Park, NC. Both ma-
terials were identical and the purity was
about 98  percent. Water, soil extracts,
and landfill leachates of varying content
of dissolved organic carbon (TOO were
collected and analyzed. The samples
used in the C-56 solubility studies and
their respective TOC values are given in
Table 1. TOC values in water samples
ranged from 0.32 to 271  ppm; this al-
lowed us to determine if solubility dif-
ferences occurred as a result of dis-
solved organic matter in water.
  The sorbents used in the sorption and
mobility studies were four clay miner-
als, seven soils, a clean sand, and some
low temperature ashed soils and muck.
The carbonaceous sorbents Ambersorb
XE-348 and activated bone carbon were
also selected for study because of their
potentially high sorption capacity for
C-56 from aqueous  solution.

Results

Solubility of C-56 in Waters
and Leachates
  The solubility of C-56 in distilled
water, deionized  water, tap water,
Sugar Creek water, soil  extracts, and
landfill leachates varied from  1.03 ppm
in Sugar Creek water to 1.25 ppm in Du-
Page landfill leachate. The results are
given in Table 1.

Effect of Dissolved Salts on the
Solubility
  The effect of several soluble salts on
the solubility of C-56 was treated by fit-
ting the solubility data to the
Setschenow equation:

                S0
            log -g- = Km

where S0  is the solubility (ppm) of C-56
in tap water and S its solubility (ppm) in
a salt solution of concentration m (mole/
L). Representative data for C-56 plotted
according to the Setschenow equation
are shown in Figure 1. Of the three salts
studied, sodium hydroxide and sodium
       chloride decreased the solubility of C-5.
       in tap water and sodium hypochlorif
       slightly increased its solubility. Th
       three salt mixtures (brine) were  intei
       mediate in  depressing C-56 solubility
       Sodium perchlorate did not signifi
       cantly affect the solubility of C-56. Th
       results indicated that there was ai
       anion  effect and that the effect  of th
       individual salts upon C-56 solubility wa
       additive.

       Photolysis and Hydrolysis
         C-56 was found to be  photoreactive
       The rate of photolysis in  aqueous solu
       tion and  organic solvents followed
       first-order reaction. Values of the first
       order rate constant (K), the half-life (tl7 ;
       and correlation coefficient (r2) betweli
       concentrations and times for the photol
       ysis of C-56 in solution exposed to sun
       light, long-wave UV, and short-wave IP
       light are given in Table 2. Representa
       tive first-order degradation plots of C-5i
       photolysis in tap water exposed to long
       and short-wave UV light are given ii
       Figure 2. C-56 photolyzed much faster ii
       hexane than in tap water or methano
         •4-\

         .5
           0
         Salt

       Figure
0.4 0.8 1.2 1.6 2.0 2.4 2.8 3.2 3.6
Concentration (mole/L)   ISGS1983

1.    Elfect of dissolved salts on the
     water solubility of C-56 at 22°C
     ±  1°C—plot according to the
     Setschenow equation.
 Table 1.   Solubility of C-56 at 22°C ± 1 °C After 15 Hours Equilibrium in Waters and Landfii
          Leachates Using Centrifugation Technique
  Waters and Leachates
TOC of Waters and
 Leachates (ppm)
             Concentration (mg/L
Distilled water
Deionized water
Tap water
Sugar Creek water
Bloom field soil extract
(soil:tap water = 1:3)
Catlin soil extract
(soil:tap water = 1:3)
Blackwell sanitary landfill
leachate
DuPage Sanitary landfill
leachate
       0.32
       0.50
       1.31
       7.62
      16.00

      49.80

     235.00

     271.00
                    1.11
                    1.14
                    1.08
                    1.03
                    1.06

                    1.20

                    1.19

                    1.25

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Table 2.    First-Order Rate Constant (K), Half-Life (t1/2), and Regression Coefficient (r2) for the
          Photodegradation of C-56 in Distilled Water, Methanol, and Hexane Under Sun-
          light, Long-Wave UV Light (L-UV), and Short-Wave UV Light (S-UV)
K (min ^) 1 1/2 (min)
Treatment
C-56 in water
C-56 in methanol
C-56 in hexane
Sun
0.24
0.30
0.45
L-UV
0.30
0.32
0.45
S-UV
0.019
0.006
0.013
Sun
3.47
2.32
1.55
L-UV
2.28
2.17
1.55
S-UV
36.47
115.50
53.31
Sun
1.00
1.00
1.00
r2
L-UV
1.00
0.99
1.00

S-UV
1.00
1.00
0.99
    .
    0.9
 I 0.61

| °o:l-
.§ 0.2"
§ 0.1
£ 0.09
§0.06

,§ 0.03
  o.or.
                      Tap Water
                      Short-Wave UV
               468
                Time (min)
               10  12  14
                ISGS 1983
 Figure 2.
First-order degradation plot of
tap water soluble C-56 exposed
under long- and short-wave UV
light.
The half-life due to photolysis of C-56 in
water exposed to long-wave UV or sun-
light was less  than  3.5 minutes (Fig-
ure 2). The half-life of C-56 in water in
the dark was about three months at
both 22°C and 35°C, indicating little tem-
perature dependence. Although pH did
not significantly affect the C-56 hydroly-
sis rate in  aqueous solution, the pres-
ence of iron caused an increase in the
hydrolysis  rate. At least 12 products of
photolysis  and hydrolysis were  identi-
fied. Pentachlorocyclopentenone and
hexachlorocyclopentenone were the
major products found in distilled water.
Cis-  and trans-pentachlorobutadiene,
tetrachlorobutenyne, and  pentachloro-
pentadienoic acid were the major prod-
ucts identified in mineralized water.
Pentachlorocyclopentenone which is
unstable under high temperature condi-
tions in hexane solution and in water
was  dimerized  and then converted to
hexachloroindone. A postulated degra-
dation pathway of C-56 is shown in Fig-
ure 3.

Sorption by Soil Materials,
Activated Carbon, and
AmbersorbR Carbonaceous
Sorbent
  All data were fitted by linear  regres-
                            sion to the log form of the  empirical
                            Freundlich adsorption equation:

                                   log ^ = log Kf + N log C

                            where x = p.g of compound sorbed;
                            m =  weight  of  sorbent (g);
                            C = equilibrium concentration of the so-
                            lution (p-g/mL); and  Kf and N  are con-
                            stants. Values of Kf and N were obtained
                from the regression_equations as the in-
                tercepts at a concentration of 1 ppm and
                the slope, respectively. The Freundlich
                parameters and the correlation coeffi-
                cient (r2)  between the amount of C-56
                sorbed by a  unit of sorbent and  the
                equilibrium concentration of C-56  are
                shown in Table 3. The molar K (KF) was
                calculated from mass K (Kf) by using the
                equation  described by Osgerby:

                              Kf (mass) x Mol wtN
                   «F
                Bryce> muck + Catlin (1:1) > #5 soil >
                Catlin > Flanagan > Bloomfield > Ava.
                                                                Aqueous Degradation Pathway
                                                       C-56


                                                        Cl Cl
    Pentachloro-2-      Hexachloroindone
    Cyclopentenone
                                                                                          Cl    O
                                                                                        Tetrachloro-
                                                                                       Pentadienoic
                                                                                           Acid
                                     Hexachloro-3-  C(/>  c,'
                                    Cyclopentenone   \_
                                                   Cl
                                                      *
                                                      J-//C/

                                                     Cl

                                      Pentachloro-  Ct'f^ Cl
                                  Pentadienoic Acid    (^  COOH

                                                     Cl
                                                         C02
                                  Cis-Pentachloro-
                                       Butadiene
                                                 c
 Cl          Cl
-^ Cl    CI^CI-HCI
                                                     Cl
                                                                              Cl
             H
          Trans-      Tetrachloro-
       Pentachloro-     Butenyne
        Butadiene
                                                                                     ISGS 1982
                             Figure 3.    Proposed pathway for the degradation of C-56 in aqueous solution.

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Table 3.    Freundlich KF, N, Correlation Coefficient (r2), and Molar KF for Sorption C-56 by Various Sorbents from Tap Water Aqueous Solution
          at 22°C                                                                                                  1

                                                                  Freundlich parameters
       Sorbents
                                                               N
                                                                    KF
                                                             (nmole<1-">mLng-1)
Carbon Sorbents

  Activated carbon
  Ambersorb

Soil Materials
16,854
 2,706
            0.60
            0.63
0.9982
0.9736
"Average amount of C-56 sorbed by unit of sorbent (pg/g) (omitted from regression in Figure 4).
28,371
 4,381
Houghton Muck B
Muck B ashed 1 day
Muck B ashed 6 days
Muck B ashed 38 days
Bryce silty loam
Muck A + Catlin silt loam (1:1)
#5 soil
Catlin silt loam
Catlin ashed 22 days
Flanagan silt clay loam
Bloomfield loamy sand
Ava silt clay loam
Ava ashed 19 days
Ca-bentonite
Illite
Montmorillonite
Kaolinite
1,045
910
637
64
385
284
97
54
4.74*
30
14
9
1.18*
32
24
18
4.4
0.80
0.76
0.71
0.68
0.86
0.76
0.66
0.93

0.95
0.55
0.94

0.63
0.55
0.60
0.58
0.9822
0.9759
0.9641
0.9506
0.9946
0.9971
0.9725
0.9982
0.0140
0.9943
0.9085
0.9148
0.0392
0.9838
0.9991
0.9928
0.9924
1,366
1,235.8
924.5
97
462
388
151
59
4.7*
32
25
10
1.2*
52
43.1
30.3
7.6
This suggests a relationship between
the organic matter content of these soils
and their sorption capacity for C-56. The
sorption of C-56 on four clays followed
the series: Ca-bentonite > illite > mont-
morillonite > kaolinite.  C-56  was
strongly  sorbed by  activated carbon
and Ambersorb XE-348. The sorption
constants (Kf) for activated carbon and
Ambersorb XE-348  were 16,854 and
2,706,  respectively.  The results  indi-
cated that activated carbon and Amber-
sorb XE-348 were much more effective
than most earth materials in removing
C-56 from water.

Effect of Earth Material TOO on
Sorption
  The relationship between the total or-
ganic carbon (TOC) content of 15 earth
materials used as sorbents and sorption
of C-56 (Table 3) was investigated. The
molar K (KF) plotted as a function of TOC
(percentages) is shown in Figure 4. A
high correlation (r2) between KF and
TOC (%) was found with a linear regres-
sion relation of:

         KF = 42.65  TOC (%)
               (r2 = 0.972)
         Koc = 4,265
 The slope of the line from Figure 4
 yields a new sorption constant (Koc) nor-
 malized for the organic carbon content
 of the soils. The K^ determined for C-56
 was approximately 4,265. The results
 indicated that the sorption properties of
 soil materials for C-56 can be predicted
 relatively accurately when the TOC con-
 tent of the involved earth materials is
 known. However, only a few soil materi-
 als was used to  develop the equation
 and the relationship between sorption
 and types of organic matter is unknown.
 Even though the overall relationship is
 highly significant, serious discrepancies
                      Kf = 42.65 TOC
                      r2 = 0.97
                        = 4,265
                                 30
                 TOC(%)
 Figure 4.
Kr vs. TOC for C-56 sorption by
15 soil materials.
between predicted and actual sorption
for a given soil may occur. Therefore,
caution should be used in  interpreting
and using these results.

Effect of Soluble Salts on
Sorption
  The  results showed that  all the salts
and brine dramatically affected sorp-
tion. The change in sorption followed
the inverse trend of the Setschenow
parameters (K). Salts causing the great-
est depression in  solubility also caused
the largest increase in sorption and vice
versa. The effect of soluble salts on C-56
solubility appears to be related to its
sorption by soil materials.


Mobility of C-56 in Soils:
Determination by Soil TLC
  The  mobility  of C-56 in soils, ex
pressed as Rf values, is summarized  ir
Table 4. According to the results, C-56
stayed immobile  in  all soils  wher
leached with tap  water, caustic brines
or landfill leachate. However, C-56 was
highly mobile when  leached with or
ganic fluids such  as acetone/water mix
tures,  acetone, methanol,  or dioxane
The mobility of C-56 increased wher

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 Table 4.   Mobility of Hexachlorocyclopentadiene in Several Soil Materials Leached With Various Solvents as Measured by Soil TLC

                                                             Rf Value'
Soil
Materials
Muck soil
Catlin silt
loam
Flanagan silt
clay loam
Ava silt clay
loam
Bloomfield
limestone
Ottawa sand
Caustic
12-4-3
/VD*'*
0.00

ND

0.00

0.00

ND
Brine (%)**
3-1-0
ND
0.00

ND

0.00

0.00

ND
Tap
water
0.00
0.00

0.00

0.00

0.00

ND"
Landfill
leachate
0.00
0.00

0.00

0.00

0.00

0.00
Acetone/
water (1:1)
0.09
0.79

0.84

0.87

0.87

0.90
Methanol
0.05
0.88

0.89

0.89

0.97

ND
Acetone
0.14
0.97

0.99

0.99

0.98

ND
Dioxane
0.64
0.99

0.99

0.96

0.99

0.98
   'Computed from statistical peak analysis of data by using values of 1st moment for grouped data.
  "NaCI - NaOCI - NaOH (in tap water).
 "'ND = Not determined.
leached with acetone/water mixtures as
the percentages of acetone increased in
water. Mobility of C-56 in soil was pro-
portional to the solubility of C-56 in the
solvent and to the soil organic content.
C-56 was  significantly more mobile in
sandy soil than in muck soil.
  The above findings are significant in
C-56 waste disposal. To decrease  the
risk of C-56 migration  from a landfill,
C-56 wastes should not come in contact
with leaching organic solvents or highly
organic leachates. This study also sug-
gests that migration of C-56 through  soil
as vapor transport may be ah important
mechanism.

So/7 Column Leaching Study
  In this study, a sample of Bloomfield
loamy sand heavily spiked with C-56 to
simulate a spill was leached  with  192
inches of tap water. We found that
0.0005 percent of the  applied com-
pound was leached  from  the soil
column. This loss suggests that C-56
will not be readily leached from even
highly contaminated soils by percolat-
ing water.  However, some hydrolysis
products apparently have higher solu-
bility in water than C-56 and were much
more  mobile in soil. From this study, it
was concluded that the secondary prod-
ucts of C-56,  rather than C-56 itself,
might migrate and cause environmental
problems.

Recommendations
  The results of this study showed that
C-56 was photoreactive  and subject to
hydrolysis and sorption reactions. How-
ever, the disappearance of C-56 from
the environment does not mean that it
is always degraded to smaller
molecules or sorbed to soil particles.
C-56 has also been shown to condense
into compounds of  larger molecular
weight.  To determine the full environ-
mental impacts of C-56 on aquatic sys-
tems, further study of the toxicity and
mobility of C-56 breakdown and/or con-
densation  products is essential.
  Almost no attenuation of C-56 by soils
occurred when  soil contaminated with
C-56 was leached with organic solvents.
To decrease the risk of potential migra-
tion of C-56 from a landfill, C-56 wastes
and organic solvents should not be dis-
posed of in the same landfill location
and C-56 waste should not come in con-
tact with leaching  organic solvents or
highly organic leachates. Additional re-
search is also needed to determine the
chemical transformation of C-56 in soils,.
in real environmental  settings,  espe-
cially in landfills where buried C-56
wastes  may come in contact with or-
ganic solvents, acids, or iron drums.
  The results and conclusions derived
from this study deal specifically with at-
tenuation and mobility of C-56 in the liq-
uid phase. Vapor phase transport
through  soil pores was ignored; for
compounds such as C-56 which have an
appreciable  vapor pressure—this
means of migration may be a significant
mechanism for redistribution. More in-
formation is needed to assess the mag-
nitude of this means of migration for all
organic wastes including C-56.
  The full report was submitted in par-
tial fulfillment of Grant No. R806335 by
the Illinois State Geological Survey. The
report, entitled "Soil, Clay, and Caustic
Soda  Effects on Solubility, Sorption,
and Mobility of Hexachlorocyclopenta-
diene," was published as Environmen-
tal Geology Note  104 by the Illinois
State Geological Survey.
  The Principal Investigators, S. F. Joseph Chou and Robert A. Griffin, are with the
    Illinois State Geological Survey, Champaign. IL 61820.
  Mike H. Roulier is the EPA Project Officer (see below).
  The complete report, entitled "Soil, Clay and Caustic Soda Effects on Solubility,
    Sorption, and Mobility of Hexachlorocyclopentadiene," (Order No. PB 84-116
    060; Cost: $11.95, subject to change) will be available only from:
          National Technical Information Service
          5285 Port Royal Road
          Springfield, VA 22161
          Telephone: 703-487-4650
  The EPA Project Officer can be contacted at:
          Hazardous Waste Engineering Research Laboratory
          U.S. Environmental Protection Agency
          Cincinnati, OH 45268

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