United States
                     Environmental Protection
                     Agency
Robert S. Kerr Environmental
Research Laboratory
Ada OK 74820
                     Research and Development
EPA-600/S2-83-004 Apr. 1983
&EPA          Project  Summary

                     Swine  Lagoon  Effluent
                     Applied  to Coastal
                     Bermudagrass
                     Philip W. Westerman, Joseph C. Burns, Larry D. King, Michael R. Overcash,
                     and Robert 0. Evans
                       The utilization potential and the envi-
                     ronmental effects of applying  swine
                     lagoon  effluent to Coastal  bermuda-
                     grass were evaluated for six years.
                     Lagoon effluent was applied to 9 m x 9
                     m plots by weekly sprinkler irrigations
                     during the growing season. A random-
                     ized block design with three application
                     rates based on nitrogen (N) (about 335,
                     670 and 1,340 kg N/ha/yr) was util-
                     ized. The high rate treatment resulted in
                     application of N.  phosphorus (P)  and
                     potassium (K) at about five, thirteen,
                     and eleven times, respectively,  the
                     normally recommended fertilizer appli-
                     cation rates for high yields of hay.
                       Forage yield and quality, soil nutrient
                     levels and water quality and quantity of
                     runoff and subsurface lateral flow were
                     evaluated. An intake trial with ewes
                     was also conducted to determine ani-
                     mal acceptance of hay from lagoon-
                     irrigated treatments.
                       The results indicated that swine la-
                     goon effluent can be an excellent source
                     of nutrients for Coastal bermudagrass,
                     but water quality considerations, nitrate
                     levels in the forage, and long-term soil
                     effects must be evaluated when deter-
                     mining acceptable maximum applica-
                     tion rates, which  is important when
                     land area for application is limiting.
                       This Project Summary was developed
                     by EPA's Robert S. Kerr Environmental
                     Research Laboratory, Ada, OK, to an-
                     nounce  key  findings of the research
                     project that is fully documented in a
                     separate report of the same title (see
Project Report ordering information at
back).*

Introduction
  Swine production systems which utilize
anaerobic lagoons usually require a land
receiver system  for lagoon effluent to
avoid lagoon overflow. The ability of a
soil-plant receiver system to utilize ap-
plied lagoon effluent depends primarily
upon the crop and the soil chemical and
physical properties. Climate, lagoon ef-
fluent application rate, and effluent com-
position also affect the utilization.
  The design of the lagoon and the soil-
plant receiver system depends largely on
whether the producer's main objective is
(1) manure treatment and disposal or (2)
utilization of manure nutrients for useful
crops. If limited  by land, the producer
would want maximum lagoon treatment
and effluent  applied to the soil-plant
receiver system at maximum rates. The
effluent rates could be sustained without
causing toxicity to plants, failure of soil
structure, or  excessive degradation of
ground water and rainfall runoff. Also, if
the plants are to  be fed to animals, the
mineral and metal composition must not
reach toxic levels. On the other hand, if
land  is not limited, and the producer
utilizes the lagoon effluent for crop irri-
•Although the research described in this article has
 been funded wholly or in part by the United States
 Environmental Protection Agency through grant R-
 804608 to North Carolina State University, it has not
 been subjected to the Agency's required peer and
 policy review and therefore does not necessarily
 reflect the views of the Agency, and no official
 endorsement should be inferred.

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gation and fertilization, the lagoon may be
designed to minimize  nutrient losses
while effluent is applied at rates based on
efficient crop utilization of nutrients.
Then the producer must decide whether
to base application rate on N, P or another
element. Typically, if N is the base ele-
ment, P and K are applied in excess of
plant utilization. However, if P is the base
element, additional N must be  applied
using commercial fertilizer. Thus, depend-
ing upon the producer's objectives and
the land restrictions, a  wide range of
nutrient loading rates may be found in
practice. Whether the maximum rate is
limited by detrimental effects to crop, or
soil, or by water quality of ground water
and runoff must be determined.
  One crop which is receptive to irriga-
tion of swine  lagoon  effluent in  the
Southeast is Coastal bermudagrass (Cyno-
don dactylon L Pers). This bermudagrass
is a deep-rooted,  long-lived  perennial
which  grows well in hot weather and
requires a well-drained soil. Also  it can
remove relatively  large amounts of N
which is often used as the base element
for determining effluent application rates.
In this study,  the plant-soil receiver
system was Coastal bermudagrass grow-
ing on a Norfolk sandy loam soil. Lagoon
effluent was applied during the crop
growing season to  replicated plots at
three N levels ranging from a fertilization
rate normally  recommended for high
yields to a rate five times higher.
   Results are presented for six years of
monitoring irrigation applications, crop
yield  and composition, soil  cores,  and
runoff. Also included is a 20-month
period of monitoring subsurface flow on
three plots. Because most studies of this
type have covered only one to three years,
the study demonstrates long-term effects
which may not be evident in short-term
studies, especially in regard to soil accu-
mulation and water transport of possible
pollutants.

Conclusions
   Weekly irrigation of swine  lagoon ef-
fluent  to Coastal bermudagrass during
the growing season resulted in excellent
crop response. Yields increased with
increased  application rates, but  there
was little advantage in dry matter produc-
tion from applying N above the medium
rate (670 kg N/ha/yr). Applying N,  P, and
K up to five, thirteen, and eleven times,
respectively, the normal recommended
rates for  high  hay yields under   non-
irrigated conditions did not result  in any
significant problem with forage quality or
soil physical structure for the six years of
the study. The  only exception was the
possible hazardous animal intake levels
of  nitrate  nitrogen  (N03-N)   in
forage from the highest-rate treatment.
Soil sampling results indicated that con-
tinued application at the two  highest
rates  could cause some nutrient imbal-
ances due to  high P accumulation (e.g.,
reduced iron  [fe] uptake), and periodic
liming would probably be needed  to
correct for calcium (Ca) and magnesium
(Mg)  losses from the  topsoil and the
decreased pH. The Ca and Mg deficiencies
may occur even though large amounts of
these and other minor  elements are
applied with the lagoon effluent.
  Transport of nutrients in surface runoff
was relatively low because of the sandy
topsoil and low slope of the plots. Greater
transport generally occurred in subsur-
face drainage for this layered soil. Be-
cause the high nutrient concentrations in
surface and subsurface flow and high
transport of N03-N in  subsurface flow
from the highest two treatment rates is
apt to be unacceptable in most situations
related to water quality, water pollution
concerns will probably govern the appli-
cation rate for disposal of lagoon effluent.
Keeping application rates near the low-
rate treatment (near normal crop fertil-
ization) would utilize a greater percentage
of the nutrients and be more acceptable
environmentally.

Recommendations

  After six years of applying swine lagoon
effluent to Coastal bermudagrass,  no
significant detrimental soil effects  or
nutrient imbalances in plant uptake were
evident even when nutrients were applied
at several  times the normal fertilization
rates. However, some  trends indicated
potential agronomic problems if high-rate
applications continued, and the water
quality of  surface runoff and drainage
from the plots receiving high-rate appli-
cations  was of environmental concern.
Some of these trends were not evident
after the first two to three years, which is
the normal duration of studies of this
type.  Thus, researchers  and research
funding agencies should set priorities to
allow for some long-term  studies of this
type. Some recommended research areas
are:
 1.  Studies of ten years or longer dura-
    tion should be conducted to deter-
    mine long-term effects of continuing
    excess applications of nutrients with
    livestock  and poultry manures and
    lagoon effluent. Various plant-soil
    receiver systems should be studied  *
    because soil changes, plant nutrient
    imbalances, and water quality effects
    will vary with soil type, plant type and
    hydrologic conditions. Thus, the nu-
    trient upon which application rates
    should be based can vary from system
    to system.
2.  The application losses of N by NH3-N
    volatilization and the soil reduction of
    NO3-N by  denitrification needs fur-
    ther study, particularly with lagoon
    effluent irrigation systems. NH3-N
    losses and mineralization rate  of
    organic nitrogen applied need fur-
    ther study  in order to compare avail-
    ability rates in these systems with
    that in agronomic systems using commer-
    cial inorganic  fertilizer.  Also, it  is
    difficult to determine how much  of
    the N in the soil is denitrified and how
    much is transported as N03-N  by
    lateral soil-water flow and deep
    seepage and thus is a potential water
    quality problem.
3.  Long-term studies of this type should
    be conducted with other crops, other
    soils, and other management strate-
    gies  such  as  year-round irrigation,
    less frequent irrigation,  and basing
    lagoon effluent application rate on P
    or  K  and adding supplemental N  in
    commercial fertilizer. Economics  of
    alternatives should be evaluated.
4.  Actual field-size systems should  be
    studied, including evaluation of im-
    pact on water quality of ground water
    and nearby streams.

Crop  Response

  The Coastal  bermudagrass was evalu-
ated for dry  matter  yield, elemental
composition,  and estimated  nutritive
value. One major goal was to determine
the quantity of N and other constituents
that could  be  deposited in Coastal ber-
mudagrass forage without adversely af-
fecting stands  or forage quality.
  Dry matter yields are shown in Figure
1. The highest N  rate produced the
greatest  dry matter yields but was not
statistically  different from the medium
rate. Both  the high and  medium rates
produced greater yields than the low rate.
The seven-year mean dry matter yields
were 10,750, 14,230 and 15,810 kg/ha
for the low, medium, and high treatments,
respectively. The low N rate with irriga-
tion showed about a 25% increase in yield
over the  non-irrigated plot receiving
similar N amounts. Irrigated effluent
amounts were approximately 12, 24 and
48 cm/yr for the low, medium, and high

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  20

  18

  16

   a
     -Medium
- Grazed
         A Irrigated With Lagoon Effluent
         O Non-Irrigated, Fertilized
                   Year
Figure  1.    Dry matter yields of Coastal
           bermudagrass.

treatments, respectively, and irrigations
were weekly from April through Septem-
ber. The simulated hay and grazed plots
received 336 and 200 kg N/ha/yr, re-
spectively. The yield data  indicate  little
advantage in dry matter production from
applying N above the medium rate (670kg
N/ha/yr). Also, there was some evidence
that bermudagrass plots  receiving the
medium and high  loading rates were
more prone to winter injury, and would
therefore require more re-sprigging  to
maintain stands.
     Concentrations of minerals in the
bermudagrass generally increased with
increased application rates of effluent.
However, the concentration  increases
were generally nonlinear and showed a
plateauing between the medium-rate and
high-rate treatments. Elements which
showed potential for increased concen-
trations with even higher  effluent rates
were P and manganese (Mn).
  Nitrogen concentration  in the forage
averaged 2.24%,  2.77%, and 2.95% for
the  low,  medium and high-rate treat-
ments, respectively.  However,  it  was
expected that higher concentrations of N
(3 to 4%) would result from the  high
loading rate. The increases in the N
concentrations are important because of
(1) interest in maximum crop uptake of N
and (2) increased use of Coastal  ber-
mudagrass meal as a source of protein
and vitamins  in  livestock and poultry
feeds.
  The concentrations of elements in the
bermudagrass were generally adequate
when compared to the requirements for
growing and finishing steers. The crude
protein values (11 to 20%) were adequate
to meet N requirements for most rumi-
nants. The nitrate nitrogen (NOa-N) con-
centrations, monitored on a limited basis
for one year, were in the toxic range on
the high-rate  treatment.  More N03-N
data is needed but  the limited results
indicate that  forage from the high-rate
treatment would  probably need to  be
blended with other feeds  to reduce the
NOa-N concentrations.  Concerning  for-
age acceptability, an intake trial with
ewes showed no difference in hay intake
between the control and the low, medium,
and high treatments.
  The average annual quantities of N and
P  removed in the bermudagrass  are
shown in Table 1. Although the amounts
of N and P  increased  with increased
effluent rates, the percentage recovery of
N and P applied decreased. The amounts
not recovered in forage were very high for
the high-rate treatment  and  indicate
potential problems with soil accumulation
of P and movement of NOa-N to ground-
water.
                                   Soil Effects
                                     Soil  levels of NOa-N  increased with
                                   increased loading rate  (Figure  2). The
                                   sandy texture of the upper 30 cm of the
                                   profile resulted in little N03-N increase in
                                   this zone normally, but accumulation
                                   occurred in the  subsoil. Accumulation of
                                   NOa-N in the subsoil has been noted in
                                   soils of the Southeastern United States,

                                    Table 1.    Amounts of N and P in Forage
and is thought to be a result of the weak
adsorption of NOa-N ions in acid subsoils
high in aluminum (Al) and iron  (Fe)
oxides and a result of non-uniform water
movement in  the subsoil. However, the
amount of N retained  in the soil was a
relatively small percentage of  the  N
applied. Of approximately 6,800 kg/ha of
N  applied to the high-rate treatment
during the  six-year period, only about
12% remained in the soil and most of this
was NOa-N. Since crop removal averaged
only 34% of the amount applied on the
high-rate treatment, a  large amount of N
is  lost by leaching, lateral subsurface
flow,  and/or denitrification when the
application rate  is this  high.
  The effect of  effluent application  rate
on concentrations of dilute acid extract-
able soil P was generally in the order high
rate > medium rate = low rate. By the
sixth year, differences were significant in
the 30-60 cm layer, indicating a signifi-
cant downward  movement of P. For this
six-year period, application of P in excess
of  crop removal  was about 1,500 kg/ha.
Continual application  of excess P could
cause  nutrient  imbalance such  as re-
duced Fe uptake.
  Irrigation of effluent tended to decrease
pH and levels of Ca and Mg inthetopsoil.
These changes could be counteracted by
periodic additions of dolomitic limestone
to the soil. Effluent applications had little
or  no effect on soil copper (Cu), zinc (Zn)
and Mn.
  Potassium and sodium (Na)  accumu-
lated  in  the  subsoil  on  the high-rate
treatments. However,  the low pH (4.2  -
4.6) and low exchangeable Na (approxi-
mately 6% NA saturation) in the zone of
Identification



Amount applied
Amount in harvested forage
Amount not recovered
Percent recovered in forage



Amount applied
Amount in harvested forage
Amount not recovered
Treatment



336
174
162
52



37
16
21
Low
N
- Irn/ha -

338
247
91
. 	 o/0 	
73
P
kn/hst

78
32
46
	 % 	
Medium



670
382
288
57



153
43
110
High



1.337
450
887
34



301
52
249
                                    Percent recovered in forage
                                     43
                                                   41
                       28
                                                                         17

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!
                                                       30
                                                              35
40
                                                                             45
Figure 2.
                      15    20      25
                          Soil NO's-N,
Effect of lagoon effluent irrigation rates on soilNO3-N. Treatments with same letter
(at same depth) are not significantly different at the 5% level. The control sample
(CON) was obtained from adjacent, non-irrigated area fertilized at maintenance
levels.
             highest Na concentration (40 ppm at 210-
             240 cm) precluded any loss of soil hydrau-
             lic conductivity due to Na induced clay
             dispersion, even though calculation of
             the ratio of Na and K applied to total salts
             applied would predict possible dispersion
             problems.

             Surface Runoff
               The  sprinkler irrigation system  was
             usually activated each week of the grow-
             ing season  without regard to rainfall.
             Consequently, some irrigation runoff and
             irrigation-rainfall mixed runoff occurred,
             and this runoff was very high in nutrients
             and oxygen demand. This type of runoff
             could probably be avoided by withholding
             irrigation when soil moisture is high or
             when rainfall is expected.
               Total runoff (including any irrigation
             runoff) averaged less than 10% of annual
             rainfall plus irrigation, which was reason-
             able for these plots with low slope (1 -3%)
             and sandy topsoil. However, concentra-
             tions of nutrients in  runoff were  high
             compared to most agricultural runoff. The
             mean concentrations  of total N, NOa-N
             and P in rainfall runoff (without irrigation
             runoff) over the six-year period are given
             in Table 2. Although there was generally
             an increase in concentration of all  nutri-
             ents with higher effluent rates, only P had
             a significant increase at the 5% level for
             the high-rate treatment.
               Annual mass transport of nutrients in
             runoff was variable and treatment effects
             were seldom significantly different. Mass
             transport by rainfall runoff (no irrigation
             runoff) was very low compared to nutrient
             loading rates, e.g. generally less than 1 %
             forN.
               The  overall potential environmental
             impact of runoff of the quality measured
             for the irrigation treatments would de-
             pend on the  particular hydrologic  situa-
             tion and whether concentration or mass
             transport was the more important.  Nutri-
             ent concentrations were sometimes high
                                                                                  Table 2.
                                                                                   Nutrient
                       Rainfall Runoff Volume-
                       Weighted Concentrations

                             Concentration, mg/l
                         Low
                         Tmt.
                                                                                           Medium
                                                                                             Tmt.
                               High
                               Tmt.
Total N    7.3 a %

NO3-N     2.7 a

P         2.0 b
10.2 a     17.3 a

 4.2 a      9.8 a

 3.4 b      6.0 a
                                                                                   %Treatments with same letter are not signifi-
                                                                                   cantly different at the 5% level.

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but total runoff and total nutrient mass
transport were relatively low.

Subsurface Runoff
  Subsurface  lateral flow  collected  in
drain tubes at the interface of the A and B
horizons on three plots was much greater
in volume than surface runoff for a 20-
month period of data. Estimated annual
subsurface runoff for a two-year period
averaged about 30-45 cm; surface runoff
averaged about 1 cm. For layered soils,
quality of subsurface lateral flow  should
be evaluated since it represents a larger
flow volume than surface runoff.
  Duration of individual subsurface run-
off events ranged from one to eight days.
For a 20-month period, subsurface flow
occurred about 15% of the period and
volume was about 25% of  rainfall plus
irrigation during this period.
  Monthly mean concentrations of NOs-N
increased with increased loading  rate of
effluent. Concentrations from the medium-
rate and high-rate treatments were usu-
ally between 10 and 30 mg/l. The relative
impact of this subsurface flow on quality
of water in the surrounding area would
depend mainly upon dilution ratios and
denitrification rates. Concentrations from
the low-rate treatment were  less than 10
mg/l.
  Phosphorus concentrations were usu-
ally in the range of 0.1 to 0.3 mg/l for the
low-rate and medium-rate  treatments,
and in the range of 0.3  to 1 mg/l  for the
high-rate treatment. High applications of
effluent promoted  P  movement in sub-
surface flow.
  Annual mass transport of N03-N, CI"
and P  in subsurface flow (Table  3) are
based upon the 20 months of data. Mass
transport of NO3-N  was about  8%  of
applied N for all three treatments. For the
high-rate treatment, the estimated an-
nual N03-N transport was  115  kg/ha.
This represents a high nutrient transport,
but the relative water quality impact
would depend on the particular hydrologic
situation. The P:N ratio in subsurface flow
was about 3:100, but was variable.
  Overall, the  nutrient concentrations
and mass transport measured for sub-
surface drainage from the interface of the
A and B horizons indicate that applying
swine lagoon effluent at fertilization rates
of 670 and 1,340 kg N/ha would likely be
detrimental to quality of soil-water inter-
flow and ground water in the area. Nitrate
nitrogen would probably be the limiting
factor, but long-term applications could
result in considerable P movement.
   Philip W. Westerman, Joseph C. Burns, Larry D. King, Michael R. Overcash, and
     Robert 0. Evans are with North Carolina State University, Raleigh, NC 27650.
   R. Douglas Kreis is the EPA Project Officer (see below).
   The complete report, entitled "Swine Lagoon Effluent Applied to Coastal Bermuda-
     grass," (Order No. PB 83-162 264; Cost: $19.00, subject to change) will be
     available only from:
          National Technical Information Service
          5285 Port Royal Road
          Springfield, VA 22161
          Telephone: 703-487-4650
   The EPA Project Officer can be contacted at:
          Robert S. Kerr Environmental Research Laboratory
          U.S. Environmental Protection Agency
          P.O. Box If98
          Ada, OK 74820
Table 3.   Annual NOy-N. CI" and P
          Transport in Subsurface Runoff

            Estimated Annual Transport
                    kg/ha/yr
Nutrient
NO3-N
cr
P
Low
Tmt.
18
37
0.9
Medium
Tmt.
58
60
1.0
High
Tmt.
115
108
3.7
                                                                              •fr U. S. GOVERNMENT PRINTING OFFICE: 1983/659-095/1925

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Information
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                                                  OQ0032P
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