United States
                    Environmental Protection
                    Agency
Robert S. Kerr Environmental
Research Laboratory
Ada OK 74820
                    Research and Development
EPA-600/S2-83-071  Nov. 1983
&ERA         Project  Summary
                   Effect of Animal  Grazing on
                   Water  Quality  of  Nonpoint
                   Runoff  in  the Pacific
                   Northwest
                   Keith E. Saxton, Lloyd F. Elliott, Robert I. Papendick,
                   Michael D. Jawson, and David H. Fortier
                     This study was conducted to evaluate
                   the effects of cattle grazing on runoff
                   volumes and rates, erosion and sedi-
                   mentation, and chemical and bacteria
                   concentrations and quantities in the
                   surface runoff discharged from agri-
                   cultural watersheds in the principle
                   grazing areas of the Pacific Northwest.
                   The purpose  of this study was to
                   document these water quantity and
                   quality variables over a period of 3 years
                   from a  small watershed managed in a
                   typical  fashion for this region under
                   sustained economic production with-
                   out obvious resource abuse. The effects
                   of management alternatives were not
                   evaluated, but the impact  of cattle
                   presence was defined by excluding
                   grazing on a  smaller, but otherwise
                   similar, grazed watershed. Although a
                   wide variety of water quality parameters
                   were measured on the analyzed sam-
                   ples (approximately 23 types of deter-
                   minations), special emphasis was given
                   to the bacterial analyses because the
                   animal presence was expected to have
                   more effect on these than on any other
                   parameter.
                     Streamflow water quality was inten-
                   sively studied for 3 years (1976-1979)
                   on a grazed [21.5 hectares (ha)] and an
                   ungrazed check (0.9 ha) watershed near
                   Potiatch. Idaho. The objective was to
                   identify  water quantity, erosion, and
                   water quality from a typical summer
                   grazed watershed in this region of
                   winter  precipitation and to identify
                   animal impacts by comparison with the
ungrazed watershed. Special emphasis
was placed on  bacteriological water
quality measurements and interpre-
tations.
  The  study period contained a near-
drought year and two more nearly
normal years with significant runoff
that provided good  water  quality
determinations.  Erosion was  minimal
on the grazed watershed although
cattle  trails were an obvious source.
Chemicals from the grazed and un-
grazed watersheds were of low concen-
trations and quantities and the water
quality was not impaired for most uses.
Indicator bacterial numbers were often
high and were closely  related to cattle
presence on the watershed. Unexpect-
ed persistence of indicator bacteria was
found  after fall removal of the livestock
and significant numbers were found in
the spring months after the temperature
raised  and before grazing began. These
results show  that bacterial quality is
related to livestock, but there is consider-
ble doubt that indicator bacterial water
quality standards developed for point
source are appropriate for assessing
nonpoint source bacterial contamination.
More  research is needed to  identify
appropriate bacterial indicators for
nonpoint runoff. Research  is also
needed to determine the effect of
alternative pasture and grazing man-
agement on runoff quantity and quality.
  This Project Summary was developed
by EPA's Robert S. Kerr Environmental
Research Laboratory. Ada,  OK, to

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announce key findings of the research
project that is fully documented in a
separate report of the same title (see
Project Report ordering information at
back).

Introduction
  In this age of environmental aware-
ness and increased population density, it
is urgent to define and improve stream
water quality if streams are to continue to
be a source of  beauty, recreation, and
water supply. Streamflow which  ema-
nate as surface runoff from agriculture
pastureland is suspected of having less
than  desired quality because of the
obvious possibility of fecal, chemical, and
sediment contamination. Cattle  grazing
occupies a significant portion of the
agricultural landscape in much of the
nation, with cattle densities ranging from
feedlots to many hectares per animal on
the western desert ranges. Management
for  protection  of soil, vegetation, and.
water  resources ranges from (a) total
sacrifice and disregard to (b)  normal
protection for  continuous economic
protection to (c) near disuse or major
conservation efforts.
  The effect of  cattle grazing  on down-
stream water quality with various manage-
ment sherries is currently very poorly de-
fined. Scientific judgement would indi-
cate that the water quality and flow rates,
erosion and sediment production, chemi-
cals, and bacteria could all be  altered by
the presence of  livestock on a watershed
and  the  management practices associ-
ated with  this  agricultural operation.
However, there is no method of predicting
these effects other than through experi-
mental data obtained by scientific study.
These results, when combined with those
of related studies, will provide inferences
of cause and effects.
  The  result of not obtaining the envi-
ronmental impact knowledge of agricul-
tural production operations such as cattle
grazing could be potentially devastating
should regulations and controls be man-
dated without scientific basis. Even the
uninitiated recognize the fact  that every
operation on  agricultural  land will have
some environmental  and downstream
impact, and changes and demands for
restrictions and controls are inevitable. It
is imperative that  scientific  facts and
documented alternatives be available so
that society can make intelligent choices
on what controls, if any, are necessary.
  This study of agricultural runoff water
quality was one of four similar studies
cooperatively  sponsored  by the U.S.
Environmental Protection Agency (EPA). The
other three were located in  Ohio,
Oklahoma, and  Nebraska. Each study
location  measured water quality from
grazed pastureland, but  each  had a
different  emphasis depending upon the
staff and facilities available. This study
differed most distinctly from the others by
the fact that the study investigated the
opposing summer grazing-winter runoff
combination.
  The report which follows is a summary
description of the study objectives;
facilities and instrumentation used; data
organized by hydrology, sedimentation,
and 'water quality; and interpretations
and conclusions. Although much detail is
omitted, the summaries in the text and
appendices of the full report provide
sufficient information so that the  reader
may review the data to substantiate the
conclusions or to develop their own. This
opportunity for reinterpretation is  impor-
tant  because the implication of these
findings may likely change in the future,
but the carefully documented facts of
these observations will remain perma-
nent.

Conclusions
  The three study years encompassed a
variety of  precipitation amounts  and
event types from near drought to  a near
normal snowpack. The mean precipitation
quantity for the study period was slightly
below the long-term  average,  but in
general, the data are quite representative
for the study region and  certainly will
apply throughout a broad  region  of the
west  where winter  precipitation  and
summer grazing dominate.
  Surface runoff, whose quality was the
object of this study, was  199 and 136
millimeters (mm) of the  676 and 516 mm
(near normal) of precipitation for the main
watershed for the two near-normal years,
or about  20 percent of  the precipitation.
The quantity from the small, upland check
watershed  was  only one-third of this
amount. The important feature was that
adequate surface runoff occurred from
both the main and check watersheds and
that the water quality of both areas was
well defined. Because of the difference in
size and  topographic setting, significant
natural differences in runoff volume were
expected and no attempt was planned or
conducted  to relate runoff quantities to
watershed treatments  of livestock graz-
ing.
  Erosion and streamf low sediment were
much less from these pasture lands than
from similar tilled agricultural  lands.
Average  sediment yields were only 382
kilograms per hectare per year (kg/ha/yr)
from  the main watershed for the study
period compared with an  average of
5,900 kg/ha/yr for a typical nearby tilled
watershed during a study period of 1961 -
65. But the check watershed averaged only
19 kg/ha/yr for the study period, thus it
showed much less erosion and sediment
transport than the main watershed. Part
of this reduced sediment from the check
watershed was obviously associated with
the reduced surface runoff, but beyond
that, the observations showed that  cattle
trails and trampling  within the  main
watershed slopes became small interceptor
ditches from  surface  flow upslope and
eroded several centimeters (cm) in depth
and width. Several  shorter trails within
the stream alluvium leading to water places
eroded even more. And the small stream
banks (most less than 25 cm) had  some
deterioration due  to  trampling during
grazing and watering. No quantitative
assessment was  made  of these  cattle
effects and they appeared to be not highly
significant to the overall water quality,
although this is certainly a quality aspect
that  could be altered by  management
techniques of controlled cattle traffic.
  Total coliforms  (TC) in  runoff  from
grazed and ungrazed areas in the Pacific
Northwest did  not correlate with the
presence or absence  of animals.  Fecal
coliform (FC) and fecal streptococcal (FS)
numbers were elevated i n runoff from the
grazed area when  cattle were  present
above that  when  they were  absent.
However, even after animals were absent
from  the area  for  several months, FC
numbers were  elevated in the runoff
above  recommended levels of 200/100
ml (FC)and 1,000/100 ml (TC)for primary
contact.  In fact,  FC  and  FS numbers
appeared to  increase from less than
100/100 ml to several thousand per 100
ml in the runoff from the grazed area for
several months  in the spring during
warm, wet weather after animals were
removed the previous fall. Almost 3 years
of cattle absence were  required for FC
numbers in runoff  from  the check
watershed to be consistently below the
maximum recommended  numbers for
primary contact. The  data indicate that
the use of conventional FC/FS ratios or
FC numbers in runoff as a measurement
of recent fecal pollution by cattle on a
grazed  area  is of  limited or no value.
FC/FS ratios of 1, which indicate recent
animal fecal pollution, were found after
animals had  been  removed for several
months.
   Nitrogen (N) and phosphorus (P)
deliveries from the watersheds were low-
-generally much lower than from areas
used for other agricultural  purposes.
Tqtal-N losses from the grazed area were
3.8  and 3.8  kg/ha during water  years

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1978 and 1979, respectively, while total-
P losses during the same periods were
1.29 and 0.93 kg/ha, respectively, with
very low losses of ortho-P. More N fell on
the grazed watershed  in precipitation
than was lost in the runoff.

Recommendations
  This  research project provided hydro-
logic, erosion, and water quality infor-
mation from  a specific site, with one
management level, and for minimal study
years.  While these baseline data are
extremely useful for general recommen-
dations that water quality is quite good
from pastureland  of moderate grazing
and management, additional research
must be conducted to determine the ef-
fect of management practices on  sedi-
ment, chemical, and bacteriological wa-
ter  quality parameters  and to establish
bacterial  water quality standards for
nonpoint sources.  Only through carefully
planned and  documented studies with
varying management levels and tech-
niques will it be possible to adequately
understand the effect of  management op-
tions on local and downstream  water
quality.
  Research  is desperately needed to
determine the meaning of  FC and FS
numbers in runoff from nonpoint sources
such as grazed areas. This study indica-
ted that these organisms multiplied on the
pasture  during warm, wet weather
several months after the animals  were
removed. Voluminous literature indicates
that pathogenic microorganisms, which
the  presence of these  organisms indi-
cates,  cannot survive for long periods
outside the warm-blooded host. While
the  numbers  of  FC and TC  in runoff
exceeded  those recommended for pri-
mary contact, the situation on the water-
shed should not present a health hazard.
Fecal coliform and TC numbers in runoff
as defined for point sources do not appear
to  be  viable  water quality criteria for
nonpoint sources.
  Chemicals such as N and P in runoff
from those areas were below levels that
would usually be of  environmental
concern.  Grazing  areas in  the Pacific
Northwest, managed as the area de-
scribed in this report, should not present
chemical environmental hazards. Future
studies should concentrate on bacterio-
logical  processes and interpretations for
potential health and environmental ha-
zards.


Experimental Design
  A paired watershed design was select-
ed to study the water quality impact of
animal grazing. The main watershed
with an area of 21.5 ha [53.1 acres (a)]
was summer grazed with management
typical of this region. A check watershed
of 0.9 ha  (2.2 a) adjacent to the main
watershed  and a part of the original
pasture was fenced to exclude all cattle.
These two watersheds were intensively
instrumented for complete hydrologic,
sediment,  and water quality measure-
ments, and were continuously monitored
for the period December 1976 to July
1979.
  The watersheds were carefully select-
ed to be generally representative of the
partially forested grazing areas of the
Pacific Northwest. The level of recent and
current management was of particular
interest because management was not to
be varied but was to be typical (i.e. most
representative) such that neither the
worst nor best case was represented. The
areas studied met these criteria.
  The pasture was grazed at a rate that
fully utilized the grass production which
produced overgrazing in dryer years but
was not  beyond sustained use over a
period of years. Little management  for
grazing distribution or to  prevent cattle
contact with the stream was done, but
this is typical for  this region  of open
pasture, summer grazing. The cattle were
usually removed in  late October and
returned to the  pasture in late May. The
actual watershed management was
carefully recorded and monitored through
cattle numbers, forage production and
utilization, cattle habits and movements,
and dung distribution.
  The small check watershed was fenced
at the outset of the study and grazing was
prohibited during the remainder of the
study other than occasional deer and a calf
that broke  in for 1 day during the first
summer. No attempt was made to remove
existing manure at the study initiation,
thus the animal effects were a decaying
function  from  their last presence in
October 1976. To approximately maintain
the grass quantities similar between the
two watersheds and keep the presence of
cattle as the only major difference,
the check area  grass was moved, baled,
and removed about  midsummer each
year, after most vegetative growth had oc-
curred.


Results and  Discussion


Hydrology and Sediment
   Daily  quantities  of  precipitation,
streamflow, and sediment were meas-
ured on the main and check watersheds.
Precipitation
  For the three study years, precipitation
ranged from  near drought during the
1976-77 winter to above  normal for
1977-78, and near normal for 1978-79.
The annual values for these years are
shown in Table  1.
  Precipitation  during the three study
years averaged 65 mm (2.6 in) below the
Potlatch normal of 622 mm (24.5 in). The
first year was a severe regional drought
with very limited snow cover at any time
and  only 370  mm  (14.6  in)  on the
watershed. The two subsequent years
were  more nearly normal, but neither of
these years had a large snowpack
accumulation  because  of  mid-winter
thaws. February 1979  had the largest
accumulation of snow depth (about 45
cm) and this melt provided the largest
surface runoff  measured  during the
study. Discussions with local ranchers
indicated that normal accumulative snow
depths would be slightly greater than any
that were measured.

Streamflow
  Observed streamflow from the study
watersheds  followed the precipitation
trends very closely (Table 1). The 1976-77
winter had far below expected  runoff
amounts,  while the two subsequent
study years had more normal amounts.
The study period provided a wide variety
of flow events  in  different sequences
which make the average results appli-
cable to other situations.
  Observations drawn from these events,
daily, monthly,  and annual summaries
indicate that the check watershed had
significantly  less runoff  than the main
watershed. The one exception was the
frozen ground runoff events in 1976-77.
This is not unexpected because the check
watershed was situated on  an  upland
area with  less steep topography and no
wet bottomland. These differences are
always a problem  in paired  watershed
studies, and especially  so  when the
topography,  geologic setting, and total
areas differ  considerably. The surface
runoff of the check area was only 40 and
30 percent of the main watershed for the
1977-78 and 1978-79 seasons. Little or
no runoff was observed from its south
side except  in  frozen conditions.  The
effect of these differences on the water
quality interpretations is likely  not so
severe because the check area did have
sufficient runoff these.2 years to provide
significant surface flushing and numer-
ous sampling opportunities to contrast
simultaneously with those from the main
watershed.

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Tablet.   Annual Streamflow Summary

                          1977*
               	   Main	Check
                    1978              1979+
               Main      Check     Main     Check
Precipitation, mm
370
            676
                       516
Runoff
Volume, m3
Depth, mm
Total Flow
Surface Flow
Peak flow. I/ sec
2.451
11
10
26
308
34
34
4
38.193
178
119
1O3
422
46
46
4
40.623
189
136
145
370
41
41
3
"Measurements Nov. 4. 1976 to Sept. 30, 1977.
^Measurements Oct. 1, 1978, to July 5. 1979.

Sediment
  Sediment concentrations and  total
quantities were quite  low  for  both
watersheds  when compared  with  any
other agriculturally used  areas.  The
average concentrations for  all 3 years
from  the main watershed was 424
milligrams/liter (mg/l) (Table  2), with
the maximum  sample having  12,700
mg/l  and minimum sample  having  1
mg/l  (during baseflow).  Average  sedi-
ment yield was 382 kg/ha. These values
compare with mean concentrations and
yield of 7,400  mg/l and 5,900 kg/ha,
respectively, from wheat land in our
region (Missouri  Flat  Creek,  70 km2,
1961-1965). Thus, the grassed pasture
area is more than a full magnitude less
than that delivered from  a much larger
farmed watershed.
  The check watershed had significantly
less sediment discharge of only 19 kg/ha
corresponding with the much  less total
streamflow, but the mean concentrations
were also only 10 to 20 percent of those
from the main watershed.  The maximum
sampled concentration was 2,105 mg/1.
The  much lower concentrations  and
yields may partially be influenced by the
topographic setting, but the observations
indicate that much of this difference  is
attributed to the presence  of cattle on the
main watershed. Many cattle trails were
developed throughout the main water-
shed, and they  were especially concen-

Table 2.    Annual Sediment Summary
             trated in the spring and channel area
             where watering occurred. Several trails
             in this mid-watershed area intercepted the
             surface runoff and became the controlling
             channels. They eroded significantly (5 to
             10 cm in depth), and this sediment went
             directly into the small streams.
               The stream immediately above the
             main gaging location had small (15 to 30
             cm) banks that were largely unprotected by
             vegetation. Through watering and grazing,
             the cattle added some disturbance to this
             area which added to the natural tendency
             for  this small channel  to erode.  The
             upland  channel directly  west of the
             weather station also had some exposed
             banks and a small overfall which eroded
             some during the study. Again, there was
             some cattle activity in  this channel
             which undoubtedly aggravated the ero-
             sion.  Beyond the trails  and channels,
             there was  little  visible erosion.  These
             upland grass areas probably  produced
             amounts similar  to those  measured on
             the check watershed where there were
             no  apparent  areas exposed from good
             grass vegetation.

             Manure Distribution
               To provide direct measurements of the
             manure distribution on the main water-
             shed, counts were made three different
             dates during 1978 at early  summer,
             midsummer, and just  after cattle were
             removed. The watershed was stratified
                      7977*          1978          /979f        3-yr avg
                   Main  Check   Main  Check  Main   Check   Main   Check
Precipitation, mm         370

Surface Runoff, mm     10
          676
                  516
34
119
46
136
41
                         517
                                        88
40
Sediment
Yield, kg
Yield, kg/ha
Avg. cone., mg/l

877
41
408

15
16
49

10,489
488
410

25
28
60

13.291
618
455

12
13
32

8.219
382
424

17
19
47
"Measurements Nov. 4, 1976 to Sept. 30, 1977.
^Measurements Oct. 1, 1978 to July 5. 1979.
into  three sampling  zones of  (a) high
cattle use due to loafing or watering, (b)
along drainages, and (c) general grazing.
Eight sample sites were made in zone (a),
10 along the waterways, and 20 randomly
placed throughout the watershed. Each
sample consisted of counting the number
of manure drops in a  50-m2 area and
sampling the weight and  size of the
droppings. The  high  use areas  were
difficult to count due to trampling of the
droppings.
  The fecal  deposits were not evenly
distributed over the watershed, but were
concentrated in high-use areas and, to
some extent, along drainages. The high
drainage density was probably due to the
location of some animal  trails  in the
pasture. The situation is  compounded
when it is considered that two of the high-
use sites were in or immediately adjacent
to drainage bottoms.
  On an overall basis, the density of fecal
deposits across the entire watershed was
2,645 deposits/ha, providing a coverage
of 1.45 percent. Therefore, if the distribu-
tion of droppings were more uniform, the
concentrations in the drainages  would
have approached that which was present
elsewhere in the nonconcentrated areas.
In the opinion of the researchers, one
must suspect that the fecal  bacteria
counts in the runoff are a result of the
distribution of feces much more than the
overall fecal  density. This is one subject
which should be seriously considered for
additional research.

Water Chemicals and Oxygen
  During the drouth water-year (1977), N
and P losses in runoff from the main and
check watersheds were insignificant. In
water-year 1978, total  N  loss was only
3.8 and 0.48 kg/ha from the main and
check watersheds, respectively,  which
was more than during 1977 but was still
low. Nitrate concentrations were almost
negligible with a total loss of only 0.33
and  .08  kg/ha NO-j-N, respectively. N
parameters were higher from the main
watershed than the check, but no param-
eters were of sufficient magnitude to be
of environmental concern.  N concentra-
tions were high in runoff when the pond
was drained and in the initial runoff sam-
ples in the fall after the summer grazing
season. These higher concentrations
probably resulted from relatively fresh
manure particles being carried in the run-
off from the pasture and within the main
stream channel. The pond was fenced to
exclude cattle but they did have access to
the main channel below the pond to the
sampling site. Throughout this study, the
periods of greatest nutrient concentra-

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tions were associated with small runoff
volumes  such as early season events,
summer events, or pond drainage. Thus,
these events contributed very little to the
annual constituent discharge. Average
concentrations of N in the baseflow was
about one-half that of the surface runoff.
  While NOs-N losses ip runoff from the
main watershed were slightly greater in
water-year  1979 than in  1978 (1.33
kg/ha vs. 0.33 kg/ha), most N losses in
runoff were less  in 1979, thus total N
losses were similar. Again, N losses in
runoff were low and were generally
lower than  would be expected in runoff
from areas  used for other agricultural
purposes. Total N losses were greater
from the main watershed than the check
watershed.  Exchangeable sediment NH/w
was not run during water-year  1979
because  it was negligible during water-
year 1978.
  P deliveries from both watersheds
were negligible during water-year 1977.
P deliveries in runoff were very low for
water-year  1978 with runoff from the
grazed area delivering only 0.2 kg/ha/yr
ortho-P  with a total-P loss of only 1.27
kg/ha/yr.  P levels in the runoff did
increase when cattle  were present
during the  July 4 event.  Very  small
amounts of P were delivered by baseflow
even though baseflow was about one-
third of the total water lost during the
1978 water year.
  In water-year  1979, P  deliveries in
runoff and  baseflow were low from the
main and check watersheds. Generally,
average ortho-P concentrations in runoff
from the main watershed were higher in
water-year  1979 than in  1978; in both
years ortho-P in runoff decreased as the
length of time  animals  were off the
pasture  increased. Total-P lost in runoff
from the grazed watershed was slightly
greater in water-year 1978 (1.27 kg/ha)
than in water-year 1979 (0.93 kg/ha). If
baseflow was added, the  values  were
1.39 and 1.05 kg/ha, respectively. The
greater total-P loss in water-year  1978
was likely due to the December 10-15,
1977, event that had a  high  average
total-P  concentration of 3.12 mg/l.
Ortho-P and total-P losses from the check
watershed during water-year 1979 were
0.02 and 0.07 kg/ha, respectively.
  The oxygen demand, pH, SC, Cl~, and
cation delivery for the main and  check
watersheds for water-years 1977, 1978,
and 1979 are summarized in the Appen-
dix of the report. During the establish-
ment year,  water-year 1977, oxygen de-
mand for the main watershed was about
twice that of the check (probably due to
stagnation); pH for the main watershed
Table 3.    Water Quality Parameter Concentration Responses to Streamflow
Parameter
Bacteria, oxygen
demands, N and P
nutrients
Type of Runoff
Event
Rainfall
Time of Year
Fall and
spring
Response to Streamflow
Direct response to
Streamflow changes with
successively smaller •
peak concentration

It
Specific conduc-
tance, pH, Cl,
Na. K. Ca, Mg
Snowmelt or
frozen ground
rainfall
Mixed (snowmelt
followed by
rainfall)
Snowmelt or
rainfall
Snowmelt or
rainfall
Winter
Fall, winter,
and spring
Late winter
and/or
spring
Fall, winter.
and spring
Little or inverse
response to Streamflow
Little response during
snowmelt. Direct
response to rainfall
Little response to
Streamflow changes
Inverse response to
Streamflow
appeared slightly more alkaline than the
check, the SC of the main watershed was
somewhat greater than the check water-
shed, as was Cl* and cation delivery. The
oxygen demand of the  runoff from the
grazed watershed was about three times
that  of the ungrazed watershed during
water-year  1978 while Cl~ cations were
about five times greater. Values for these
parameters increased in  runoff from the
main watershed  when  cattle were
present (July 4 event). Oxygen demand of
runoff in water-year 1979 was  greater
from the grazed area than in 1978, but
runoff from  the ungrazed  area  was
similar. Cations were  not run  during
water-year  1979 because data from the
previous year  had shown  that SC was
well correlated with the Na, K, Ca, and Mg
present in the runoff.

Indicator Bacteria
  TC  counts in runoff showed  little
relationship to the presence or absence
of cattle  as shown by the summarized
results of the main and check watershed.
TC counts probably responded more to
climate than to the cattle. From results of
this study, TC counts appear to be useless
for determining stream pollution.
  In  general,  the FC and  FS numbers
decreased as expected  throughout the
winter months. Their numbers, however,
unexpectedly  increased again  in  the
spring with warmer weather.  Also, the
FC/FS ratios varied more than expected,
ranging from as high as 4/1 on the check
watershed to 1/1 on the main watershed.
  FC/FS ratios of about 0.1  to  1.0
indicate cattle fecal contamination.
Runoff contaminated with cattle normally
has a FC/FS ratio of about 0.7 while that
with  human had a FC/FS ratio of 4.0 and
higher. Animal FC/FS ratios will increase
with time, but they should remain less
than one. The high FC/FS ratios long
after cattle were removed from the area
would lead one to question the validity of
the FC/FS ratio for nonpoint areas. Fur-
thermore, when animals were introduced
back onto the watershed, the June 4,
1977, readings showed high FC and FS
numbers but a FC/FS ratio of 0.02, which
is  indicative of wild animal fecal con-
tamination. During the 3 year study peri-
od, except for a few mice and squirrels,
wildlife  populations on the study areas
were almost nil.
  The effect of grazing cattle  on runoff
water quality from the study watersheds
can be summarized by the following. TC
in runoff had an apparent relationship to
cattle grazing operations,  but  numbers
were elevated when they should not have
been. FC and FS numbers in runoff from
the grazed  watershed were  elevated
when cattle were on the pasture. After
cattle were removed, FC and FS numbers
generally declined throughout  the fall
and winter  months. However,  after
several  months absence  of cattle from
the area, FC and FS numbers  in runoff
from the grazed watershed were elevated
and in many cases exceeded suggested
wdfter  quality standards. FC and  FS
numbers appeared to increase  in runoff
from the grazed area in the  spring in
rainfall runoff following a period of warm,
dry weather several months after animals
were  removed. FC/FS ratios  in runoff
varied considerably and indicated recent
cattle fecal pollution for  long periods of
time after animals were off the grazed
area.  FS  numbers  in runoff  from the
grazed and check areas did not change
appreciably during the study. The check

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Table 4.   Average Percent Fecal Coverage in the Drainage Sampled  Within the Study
           Watershed, Nov. 4, 1978
  Sampling
   Site No.    Drainage Runoff Areas
                       Fecal Coverage
                        Percent Area
      1      Sacrifice (lower) pasture (plus rest
             of grazed main watershed)

      2      Ungrazed (check) watershed

     11      Southeast area just above mam sampling station

     12      Northeast area just above main sampling station

     13      All of the grazed watershed except the
             southeast, northeast and sacrifice areas

     14      North pasture area draining into large gulley
             channel

     15      Northwest pasture area

     16      Southwest pasture area

     17      Woodland area

     18      Logged area (outside of fenced cattle-grazed
             area; horses, however, were grazed here)
                            20*


                             0

                            NS§

                             0.7

                             2.6


                             0.9


                             3.1

                             2.2

                             1.8

                            NS
'Total remainder of watershed averaged 1.4 percent.
§/VS =Not sampled

was ungrazed from the  fall of 1976
throughout the study. FC numbers in run-
off from the  check were not consistently
below the  maximum recommended
numbers for primary contact until the
1979  water-year, although FC numbers
in the check watershed  runoff  during
water-year 1978 were genera I ly below or
close to the  recommended primary  con-
tact standard. The data would suggest
that using conventional FC/FS ratios or
FC numbers  in runoff as a measurement
of recent fecal  pollution by cattle on a
grazed area is of limited value in the Pacif-
ic Northwest.

Parameter Response and
Spatial Distribution

Parameter Response
  The type of runoff event affected the
parameter response to streamflow (Table
3).  With snowmelt on thawed ground
followed by  rain, NOa-N does not follow
the hydrograph initially during snowmelt;
however, when the ground thawed, NOs
concentration increased. Ortho-P did not
follow the hydrograph at any time during
a snowfall-rain on frozen soil event. For
the same event, COD also did not appear
to respond to streamflow until the ground
began to thaw on the second day of the
event. The water quality parameter,  in
this case total-P, did not  respond to the
streamflow  increase caused  by snow-
melt, but did respond to the later stream-
flow increase caused by rainfall runoff.
During long and large events, the check
watershed  behaved similarly. The high-
est check  watershed values often  oc-
curred in the first few runoff samples as
the  check watershed channel was
flushed since it had no base flow.  For
small events, parameter concentrations
for check runoff, therefore, did not corre-
late well with flow rates.

Spatial Distribution
  Hand samples were collected from
various locations within the watershed
during events  in the 1978 and  1979
water-years to  determine  the effect of
grazing patterns, manure  density and
land cover  on water quality parameter
concentrations.  Of specific interest was
whether the pollution load was primarily
a  result  of cattle activity in and  im-
mediately adjacent to the stream channel
(the  "sacrifice area" between the north
and south pastures) or if the stream water
quality reflected the grazing activity over
the entire watershed.
  During the 1978 water-year, samples
were taken December 6, 1977, during a
small snowmelt/rainfall event; December
13, 1977, during the largest event of the
water-year; and on March 15,1977, dur-
ing a late winter medium  sized rainfall
event.  In water-year 1979 samples were
taken from  an event caused primarily by
snowmelt on February 13, 1979, during
the largest  event of the water-year and of
the entire study; February 27, 1979, dur-
ing a large rainfall/snowmelt event; April
6, 1979, during an early spring rainfall e-
vent;  and on May 4, 1979, at the be-
ginning of a large spring rainfall event.
This last sampling occurred after indica-
tor bacterial numbers  had increased
above their late winter values.
  Fecal density  and distribution  data
were  collected during the study by the
range  management  component of the
project (see Section VI of the Range
Management Section in the report). The
amount of a drainage  area within the
pastured watershed covered  by  feces
varied from 20 percent for site 1  (sacrifice
pasture) to 0.7  percent  for  site 12
(northeast area)  at  the November 7,
1978, sampling  date  (Table  4). The
percent manure  cover  was determined
three times during the summer of  1978.
The values obtained on Nov.  7,  1978,
after the cattle were removed  from the
watershed represent the fecal  densities
present when these water  quality sam-
ples were taken  from the  1979 water-
year runoff. FC and FS numbers in runoff
from the various  sampling  sites within
the watershed did not  correlate clearly
with fecal disposition in water-year 1978
or 1979.  In fact,  no trends  are  apparent
from the sites sampled. Also, N and P in
the runoff from the various sampling sites
within the pastured watershed did not ap-
pear to agree  with  manure deposition.
Presumably vegetation and soil cover had
a greater effect on runoff nutrient content
than did manure deposition.
  These  results  would indicate that
indicator bacteria  and nutrients in runoff
from  the main   pasture areas  were
generally  equal  to  those at  the main
sampling station.  The pollution load can
be considered  nonpoint  source in  origin
and not primarily a result of the greater
grazing activity in the sacrifice area.
                                    6

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Keith E. Saxton, Lloyd F. Elliott. Robert I. Papendick, and Michael D. Jawson are
  with Washington State University. Pullman, WA 99164; DavidH. Fortier is with
  USDI. Bureau of Land Management, Coeur D'Alene, ID 83814.
R. Douglas Kreis is the EPA Project Officer (see below).
The complete report, entitled "Effect of Animal Grazing on Water Quality of
  Nonpoint Runoff in the Pacific Northwest," (Order No. PB 83-245 225; Cost:
  $14.50. subject to change) will be available only from:
        National Technical Information Service
        5285 Port Royal Road
        Springfield, VA 22161
        Telephone: 703-487-4650
The EPA Project Officer can be contacted at:
        Robert S. Kerr Environmental Research Laboratory
        U.S. Environmental Protection Agency
        P.O. Box 1198
        Ada. OK 74820

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