United States
                    Environmental Protection
                    Agency
Environmental Monitoring and
Support Laboratory
Cincinnati OH 45268
                    Research and Development
 EPA/600/S4-86/032 Jan. 1987
SEPA          Project Summary
                    Taxonomy  of  Ceriodaphnia
                     (Crustacea:  Cladocera)  in
                     U.S.  Environmental  Protection
                    Agency  Cultures
                    Dorothy B. Berner
                      This study investigated the taxonomy of
                    three groups of the cladoceran genus
                    Ceriodaphnia in cultures being used by the
                    U.S. Environmental Protection Agency.
                    One taxonomic group was identified as C.
                    reticulata. The second group was identi-
                    fied as C. dub/a. The third group was tax-
                    onomically nearly identical to C. dubla but
                    was determined to be a hitherto undescrib-
                    ed phenotypic variant of C. dubla, and is
                    designated as C, dubla, toothed-pecten
                    variety. Specimens of this form have been
                    found in populations of C. dubla collected
                    west of the Mississippi River.

                      Similarities in the general morphology,
                    postabdomens, and ephippia of C.  reti-
                    culata and C. dubia suggest that they are
                    evolutionary closely related and might be
                    able to hybridize and produce offspring
                    having an ovate-toothed pecten like that
                    of  the  C. dubia variant.  Experiments
                    designed to test this possibility were in-
                    conclusive although two successful inter-
                    specific matings were observed. It is sug-
                    gested that the relationship between these
                    two Ceriodaphnia could be further eluci-
                    dated by study of more extensive field
                    samples, and by interspecific breeding
                    experiments that include  hatching of
                    hybrid young from ephippia and study of
                    their taxonomy and fertility.

                      This Project Summary was developed
                    by EPA's Environmental Monitoring and
                    Support Laboratory, Cincinnati,  OH, to
                    announce key findings of the research pro-
                   ject that is fully documented in a separate
                    report of the same title (see Project Report
                    ordering information at back).
Introduction
  The U.S.  Environmental  Protection
Agency (EPA)  Environmental Research
Laboratory-Duluth,  the  Environmental
Monitoring and  Support  Laboratory-
Cincinnati, and other  EPA  and  private
laboratories  have  been  exploring the
suitability of Ceriodaphnia for short-term
(7 day) toxicity testing for several years.
  Initial  Ceriodaphnia  stock cultures
established during 1969 in the  Duluth
laboratory with animals obtained from fish
ponds at EPA's Fish Toxicology Station,
Newtown, OH, were identified as Cerio-
daphnia reticulata. In 1982-83, subtle dif-
ferences in the appearance of the cultured
animals suggested that the stocks com-
prised more than one species. Subsequent
microscopic  examination revealed  that
their cultures contained two, possibly
three, species of Ceriodaphnia. Two were
tentatively identified as C. reticulata and
C. dubia (or C. affinis because a problem
in synonomy exists), but the third  was
unidentifiable and appeared to have
characteristics of both the other  two.
  The goals of the study reported herein
were to verify the identification of Cerio-
daphnia in culture at the EMSL-Cincinnati
Facility, Newtown and  at  other  EPA
laboratories, and to try to determine, by
taxonomic comparison and by interspe-
cific matings, if the unidentifiable animals
were hybrids  of the two former species,
a morph of one of those species, or a new
species.
Materials and Methods
  EPA's Ceriodaphnia were compared with
specimens from other sources, including

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   specimens from the U.S. Natural History
   Museum (Smithsonian), Washington, DC,
   the British Museum of  Natural History,
   London; and  the Lilljeborg Collection,
   Uppsala, Sweden, using optical and scan-
   ning electron microscopy.

   Conclusions
     1. Nearly all Ceriodaphnia cultures at
   the EPA Newtown Facility and Duluth lab-
   oratories, and other cultures derived from
   them, were C. reticulata or C. dub/a.
     2. A third Ceriodaphnia found in a few
   EPA cultures, particularly those from EPA's
   Athens, GA, laboratory, was a morpholog-
   ical variant of C. dubia, which it resembles
   almost completely. The morph was rever-
   sibly altered to the dubia form when the
   animals were cultured in  reconstituted,
   rather than well water. The males are indis-
   tinguishable  from C.  dubia  males,
   regardless of culture medium. Specimens
   of this variant have been found in natural
   populations of  C.  dubia  west of the
   Mississippi River. It is designated  in the full
   report as C. dubia, toothed-pecten variety.
     3. Experiments attempting to  hybridize
   C. reticulata and  C. dubia were incon-
   clusive, although two successful inter-
   specific matings occurred. Taxonomically,
   these species appear to be closely enough
   related that males might mistake a female
   of the other species as their own. To ascer-
   tain  whether hybridization  is  possible,
   more experiments of the kind attempted
   in this study would have to be carried out.
   Lastly, the morphology of such hybrids
   should be compared with specimens from
   field populations in which C. reticulata and
   C. dubia coexist, to see if hybrid forms
   occur naturally.
     4. Comparison of the EPA C. dubia with
   N. American  and European populations
   designated C. dubia or C. affinis revealed
       no significant differences among them.
       This study therefore supports earlier con-
       clusions that the two names are synon-
       omous, and that  C.  dubia takes prece-
       dence over C. affinis.  It is likely, however,
       that other species of Ceriodaphnia exist
       that have characteristics similar to that of
       dubia, that should not be used to identify
       animals found in natural populations.

       Recommendations
         As an outcome of this study, the follow-
       ing recommendations were made:
         1.  There needs to be a nationally organ-
       ized and funded  program for  sampling
       freshwaters, accompanied by support for
       systematic studies, development of refer-
       ence collections,  and the publication of
       adequate taxonomic keys. Difficulties en-
       countered by EPA personnel in identifying
       the species in their cultures reflect the in-
       adequacy of the keys commonly used in
N. America to identify zooplankton. Fur-
thermore, original species' descriptions are
frequently in Latin, German, or French, are
not readily available, and may not be ap-
plicable to N. America because they are
of taxa from other continents. As the need
to monitor freshwaters increases, so does
the need for contemporary, comprehen-
sive reference collections and taxonomic
information.
  2. It is suggested that some of the key
characteristics in C. dubia are under nutri-
tional control.  This  could  possibly be
tested by rearing them in defined medium
and feeding them algae also grown in de-
fined medium. This would make it possible
to manipulate the presence and concentra-
tions of micronutrients, which seem likely
candidates for such control. Such studies
might contribute  to  the  knowledge  of
some of the factors affecting polymorph-
ism in the Daphniidae.
          Dorothy B. Berner is with Temple University, Philadelphia. PA 19122.
          William J. Horning, II, is the EPA Project Officer (see below).
          The complete report, entitled "Taxonomy of Ceriodaphnia (Crustacea:Cladocera)
            in U.S. Environmental Protection Agency Cultures." (Order No.  PB 87-110
            508/AS; Cost: $11.95, subject to change) will be available only from:
                  National Technical Information Service
                  5285 Port Royal Road
                  Springfield, VA  22161
                  Telephone: 703-487-4650
          The EPA Project Officer can be contacted at:
                  Environmental Monitoring and Support Laboratory
                  U.S. Environmental Protection Agency
                  Cincinnati, OH 45268
United States
Environmental Protection
Agency
Center for Environmental Research
Information
Cincinnati OH 45268
               U.S.OFFICIALMW£
Official Business
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