U.S. ENVIRONMENTAL PROTECTION AGENCY
        NATIONAL EUTROPHICATION SURVEY
                 WORKING PAPER SERIES
   PACIFIC NORTHWEST ENVIRONMENTAL RESEARCH LABORATORY
                 An Associate Laboratory of the
      NATIONAL ENVIRONMENTAL RESEARCH CENTER - CORVALLIS, OREGON
                          and
NATIONAL ENVIRONMENTAL RESEARCH CENTER - LAS VEGAS, NEVADA

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                                    REPORT
                                      ON
                                CARD RESERVOIR
                                TUSCOLA COUNTY
                                   MICHIGAN
                                 EPA REGION V
                             WORKING PAPER No,  190
       WITH THE COOPERATION OF THE
MICHIGAN DEPARTMENT OF NATURAL RESOURCES
                 AND THE
         MICHIGAN NATIONAL GUARD
             FEBRUARY, 1975
                               M.S. Environmental Protection Agw»O
                               Region 5, Library (PL-12J)
                               11 West Jackson Boufevard, 12tt!
                               CWcago.lt 60604-3590

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                               CONTENTS
                                                           Page
  Foreword                                                  ii
  List of Michigan Study Lakes                              iv
  Lake and Drainage Area Maps                              v,vi

  Sections
  I.   Conclusions                                            1
 II.   Lake and Drainage Basin Characteristics                4
III.   Lake Water Quality Summary                             5
 IV.   Nutrient Loadings                                      9
  V.   Literature Reviewed                                   14
 VI.   Appendices                                            15

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                         FOREWORD
    The National Eutrophication Survey was initiated in 1972 in
response to an Administration commitment to investigate the nation-
wide threat of accelerated eutrophication to fresh water lakes and
reservoirs.

OBJECTIVES

    The Survey was designed to develop, in conjunction with state
environmental agencies, information on nutrient sources, concentrations,
and impact on selected freshwater lakes as a basis for formulating
comprehensive and coordinated national, regional, and state management
practices relating to point-source discharge reduction and non-point
source pollution abatement in lake watersheds.

ANALYTIC APPROACH

    The mathematical and statistical procedures selected for the
Survey's eutrophication analysis are based on related concepts that:

        a.  A generalized representation or model relating
    sources, concentrations, and impacts can be constructed.

        b.  By applying measurements of relevant parameters
    associated with lake degradation, the generalized model
    can be transformed into an operational representation of
    a lake, its drainage basin, and related nutrients.

        c.  With such a transformation, an assessment of the
    potential for eutrophication control can be made.

LAKE ANALYSIS

    In this report, the first stage of evaluation of lake and water-
shed data collected from the study lake and its drainage basin is
documented.  The report is formatted to provide state environmental
agencies with specific information for basin planning [§303(e)], water
quality criteria/standards review [§303(c)], clean lakes [§314(a,b)]>
and water quality monitoring [§106 and §305(b)] activities mandated
by the Federal Water Pollution Control Act Amendments of 1972.

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    Beyond the single lake analysis, broader based correlations
between nutrient concentrations (and loading) and trophic condi-
tion are being made to advance the rationale and data base for
refinement of nutrient water quality criteria for the Nation's
fresh water lakes.  Likewise, multivariate evaluations for the
relationships between land use, nutrient export, and trophic
condition, by lake class or use, are being developed to assist
in the formulation of planning guidelines and policies by EPA
and to augment plans implementation by the states.

ACKNOWLEDGMENT

    The staff of the National Eutrophication Survey (Office of
Research & Development, U. S. Environmental Protection Agency)
expresses sincere appreciation to the Michigan Department of
Natural Resources for professional involvement and to the
Michigan National Guard for conducting the tributary sampling
phase of the Survey.

    A. Gene Gazlay, former Director, and David H. Jenkins, Acting
Director, Michigan Department of Natural Resources; and Carlos
Fetterolf, Chief Environmental Scientist, and Dennis Tierney,
Aquatic Biologist, Bureau of Water Management, Department of Natural
Resources, provided invaluable lake documentation and counsel during
the course of the Survey.  John Vogt, Chief of the Bureau of Environ-
mental Health, Michigan Department of Public Health, and his staff
were most helpful in identfying point sources and soliciting municipal
participation in the Survey.

    Major General Clarence A. Schnipke (Retired), then the Adjutant
General of Michigan, and Project Officer Colonel Albert W. Lesky,
who directed the volunteer efforts of the Michigan National Guardsmen,
are also gratefully acknowledged for their assistance to the Survey.

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                                IV
                  NATIONAL EUTROPHICATION SURVEY

                            STUDY LAKES

                         STATE OF MICHIGAN
LAKE NAME

Allegan Res.
Barton
Belleville
Betsie
Brighton
Caro Res.
Charlevoix
Chemung
Constantine Res
Crystal
Deer
Ford
Fremont
Higgins
Holloway Res.
Houghton
Jordon
Kent
Long
Macatawa
Manistee
Mona
Muskegon
Pentwater
Pere Marquette
Portage
Randall
Rogers Pond
Ross
St. Louis Res.
Sanford
Strawberry
Thompson
Thornapple
Union
White
COUNTY

Allegan
Kalamazoo
Wayne
Benzie
Livingston
Tuscola
Charlevoix
Livingston
St. Joseph
Montcalm
Marquette
Washtenaw
Newago
Roscommon
Genesee, Lapeer
Roscommon
Ionia, Barry
Oakland
St. Joseph
Ottawa
Manistee
Muskegon
Muskegon
Oceana
Mason
Houghton
Branch
Mecosta
Gladwin
Gratiot
Midland
Livingston
Livingston
Barry
Branch
Muskegon

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            CARO RESERVOIR
  Tributary Sampling  Site •  Sewage Treatment Facility
X Lake Sampling Site    (J' Direct Drainage  Area Limits
       0              5               ,0  Ml .

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 CARO  RESERVOIR
X  Lake Sampling Site
•  Sewage Treatment Facility
                  1/0 Mi.

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                          CARO RESERVOIR
                          STORE! NO. 26A1
I.  CONCLUSIONS
    A.  Trophic Condition:
            Survey data show that Caro Reservoir is eutrophic.  Of the
        35 Michigan lakes sampled in November when essentially all were
        well-mixed, 24 had less mean total phosphorus, 15 had less mean
        dissolved phosphorus, and none had a higher mean inorganic nitro-
        gen; of all 41 lakes sampled, 21 had less mean chlorophyll cu
        but only one had less mean Secchi disc transparency*.
            Survey limnologists observed abundant rooted aquatic vege-
        tation in the shallower areas of the reservoir.
    B.  Rate-Limiting Nutrient:
            A significant change in nutrients occurred in the algal assay
        sample between the time of collection and the beginning of the
        assay, and the results are not representative of conditions in
        the reservoir at the time the sample was collected (09/19/72).
            The lake data indicate nitrogen limitation in September but
        phosphorus limitation in June and November.
    C.  Nutrient Controllability:
            1.  Point sources—During the sampling year, Caro Reservoir
        received a total phosphorus load at a rate nearly ten times the
* See Appendix A.

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rate proposed by Vollenweider (in press)  as  "dangerous";  i.e.,
a eutrophic rate.  However, it is probable that Vollenweider's
model does not apply to water bodies with short hydraulic
retention times, and the hydraulic retention time of Caro Reser-
voir is certain to be very short.  For example, the maximum
depth sounded by Survey limnologists was  six feet; and, even
if it is assumed that the mean depth of the  reservoir is  also
six feet, the mean hydraulic retention time  would be less than
two days.
    Now, it is calculated that the communities of Caro and Cass
City contributed nearly 30% of the total  phosphorus load  to Caro
Reservoir during the sampling year.  In view of the flow-through
characteristic of the reservoir, it is likely that a high degree
of phosphorus control at these two sources,  including the possi-
ble industrial source (see page 10), would result in persistent
phosphorus limitation (see page 8) and improvement in the trophic
condition of Caro Reservoir, as well as provide additional pro-
tection for downstream Lake Huron.
    2.  Non-point sources (see page 12)--During the sampling year,
the phosphorus export of the Cass River was  somewhat high as com-
pared to other Michigan streams sampled.   However, the export
N/P ratio of 47/1 indicates point sources probably were not

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involved, and it appears that the geological  characteristics
of the drainage, agricultural practices, or both are the
cause of the somewhat higher export rate.
    In all, it is estimated that non-point sources, including
precipitation, contributed about 70% of the total  phosphorus
load to Caro Reservoir during the sampling year.

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II.   LAKE AND DRAINAGE BASIN  CHARACTERISTICS
     A.   Lake Morphometry  :
         1.   Surface  area:  200  acres.
         2.   Mean  depth:   unknown.
         3.   Maximum  depth:   unknown.
         4.   Volume:   unknown.
     B.   Tributary and Outlet:
         (See Appendix B for  flow  data)
         1.   Tributaries -
             Name                               Drainage  area*  Mean  flow*
             Cass  River                          612.0 mi2     302.2  cfs
             Minor tributaries &                         2
              immediate drainage -                24.7 mi       12.4  cfs_
                          Totals                 636.7 mi2     314.6  cfs
         2.   Outlet -
             Cass  River                          637.0 mi2**   314.6  cfs
     C.   Precipitation***:
         1.   Year  of  sampling:   32.8 inches.
         2.   Mean  annual:   27.8  inches.
 t Fetterolf, 1973.
 * Drainage areas are accurate within ±5%;  mean daily flows for 74% of the
   sampling sites are accurate within ±25%  and the remaining sites up to
   ±40%; and mean monthly flows, normalized mean monthly flows, and mean
   annual flows are  slightly more accurate  than mean daily flows.
 ** Includes area of lake.
 *** See Working Paper No. 1, "Survey Methods, 1972".

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III.   LAKE WATER QUALITY SUMMARY
      Caro Reservoir was sampled three times  during the open-water season
  of  1972 by means  of a pontoon-equipped Huey helicopter.   Each time,  samples
  for physical  and  chemical  parameters were collected from a  single station
  on  the lake and usually from two depths (see map, page v).   During each
  visit, a single depth-integrated (near bottom to  surface) sample was col-
  lected for phytoplankton identification and enumeration; and a similar
  sample was collected for chlorophyll ^analysis.   During the second
  visit, a single five-gallon  depth-integrated sample was  collected for
  algal  assays.   The maximum depth sampled was 4 feet.
      The results obtained are presented in full  in Appendix  C, and the
  data for the  fall  sampling period,  when the reservoir essentially was
  well-mixed, are summarized below.   Note, however, the Secchi disc
  summary is based  on all  values.
      For differences in the various  parameters at  the other  sampling
  times, refer  to Appendix C.

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A.  Physical and chemical characteristics:
    Parameter

    Temperature (Cent.)
    Dissolved oxygen (mg/1)
    Conductivity (ymhos)
    pH (units)
    Alkalinity (mg/1)
    Total P (mg/1)
    Dissolved P (mg/1)
    N02 + N03 (mg/1)
    Ammonia fmg/1)
Minimum
FALL VALUES

(11/15/72)

Mean       Median
          Maximum
3.6
9.6
850
7.9
240
0.116
0.021
3.410
0.340
3.6
9.6
850
7.9
251
0.117
0.022
3.485
0.350
3.6
9.6
850
7.9
251
0.117
0.022
3.485
0.350
3.6
9.6
850
7.9
262
0.119
0.024
3.560
0.360
                                           ALL VALUES
    Secchi disc (inches)
 14
 27
30
37

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B.  Biological  characteristics:

    1.   Phytoplankton -
        Sampling
        Date

        06/17/72
        09/19/72
        11/15/72
Domi
nant
Genera
1.
2.
3.
4.
5.


1.
2.
3.
4.
5.


1.
2.
3.
4.
5.

Stephanodiscus
Navicula
Dinobryon
Synedra
Nitzschia
Other genera
Total
Flagellates
Cyclotella
Lagarheimia
Cryptomonas
Macrinactum
Other genera
Total
Rhaphidiopsis
Lyngbya
Navicula
Oscillatoria
Achnanthes
Other genera
Number
per ml

  679
  622
  283
  181
  113
  577

2,455
2
2,
620
440
723
602
331
874
7,590

1,386
  301
   36
   24
    6
   31
                                          Total
1,784

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                             8
    2.  Chlorophyll a_ -
        (Because of instrumentation problems during the 1972 sampling,
        the following values may be in error by plus or minus 20 percent.)
        Sampling             Station                 Chlorophyll a_
        Date                 Number                  (yg/1)	
        06/17/72               01                         3.6
        09/19/72               01                        28.9
        11/15/72               01                         3.4
C.  Limiting Nutrient Study:
        There was a loss of 29% of the dissolved phosphorus and
    a 70% gain in inorganic nitrogen in the assay sample from the
    time of collection to the beginning of the assay.  As a result,
    the N/P ratio was shifted from 10/1 in the reservoir (nitrogen
    limited) to 29/1 in the assay sample (phosphorus limited).
    Consequently, the assay results are not representative of con-
    ditions in the reservoir at the time the sample was collected
    (09/19/72).
        The reservoir data indicate nitrogen limitation in September
    (N/P ratio = 10/1) but phosphorus limitation in June and Novem-
    ber (N/P = 23/1 and greater).

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IV.   NUTRIENT LOADINGS
     (See Appendix D for data)
     For the  determination  of nutrient loadings,  the  Michigan  National
 Guard collected monthly near-surface  grab samples  from each of the
 tributary sites indicated  on the map  (page vi),  except for the high
 runoff month of May when three samples were collected.  Sampling was
 begun in October, 1972, and was completed in September, 1973.
     Through  an interagency agreement, stream flow  estimates for the
 year of sampling and a "normalized" or average year  were provided by
 the Michigan District Office of the U.S.  Geological  Survey for the
 tributary sites nearest the lake.
     In this  report, nutrient loads for sampled tributaries were
 determined by using a modification of a U.S. Geological Survey
 computer program for calculating stream loadings*.   Nutrient  loading
 for "minor tributaries and immediate  drainage"  ("ZZ" of U.S.G.S.) were
 estimated by using the mean concentrations in the  Cass River  at station
 A-4 and the  mean ZZ flow.
     The operators of the Caro, Caro State Home,  and  Cass City  waste-
 water treatment plants provided monthly effluent samples and  corres-
 ponding flow data.  In this report, the loads attributed to the Cass
 River are those measured at station A-2 minus the  Cass City loads.
 *  See  Working  Paper  No.  1.

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                                 10
    The Caro State Home wastewater plant actually discharges to the
Cass River downstream from Caro Reservoir.  The analytical  data are in-
cluded in Appendix D for informational  purposes only.
    A beet-sugar processing plant at Caro operates seasonally.   Wastes
are treated in two groups of ponds on either side of the Cass River down-
stream from the Survey inlet sampling station (A-2).  Discharges from the
ponds (if any) were not sampled and may account for the phosphorus and
nitrogen loading imbalance observed during the sampling year (see page 13)
    A.  Waste Sources:
        1.  Known municipal  -
Pop.
Name
Caro

Cass City

Caro State
Home
Served*
1

1


,315

,065

856
Treatment
trickli
filter
trickli
f i 1 ter
act. si
ng

ng

udge
Mean
Flow
0.

0.

0.

(mgd)
395

383

176
Recei
Water
Cass

Cass

Cass
vi

Ri

Ri

Ri
ng

ver

ver

ver
below res<
        2.  Known industrial -
                                              Mean       Receiving
        Name_                  Treatment       Flow (mgd) Water
        Sugar plant at        ponds               ?      Cass River
         Caro (seasonal)
* Estimated on the basis of the effluent total nitrogen load of 7.5 Ibs
  per capita per year.
t Lehner, 1972.

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                                 11
    B.  Annual Total Phosphorus Loading - Average Year:
        1".  Inputs -
                                              Ibs P/         % of
            Source                            yr             total
            a.  Tributaries (non-point load) -
                Cass River                    40,320          67.2
            b.  Minor tributaries & immediate
                 drainage (non-point load) -   1,810           3.0
            c.  Known municipal STP's -
                Caro                           9,860          16.4
                Cass City                      7,990          13.3
            d.  Septic tanks* -                  ?
            e.  Known industrial -
                Sugar plant, Caro                ?
            f.  Direct precipitation** -      	3JD          <0.1
                         Total                60,010         100.0
        2.  Outputs -
            Lake outlet - Cass River          73,050
        3.  Net annual P loss - 13,040 pounds
* Probably insignificant.
** See Working Paper No. 1.

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                                 12
    C.  Annual Total Nitrogen Loading - Average Year:
        1.  Inputs -
                                              Ibs N/         % of
            Source                            y_r	         total
            a.  Tributaries (non-point load) -
                Cass River                  1,904,140         93.2
            b.  Minor tributaries & immediate
                 drainage (non-point load) -   91,710          4.5
            c.  Known municipal  STP's -
                Caro                           26,950          1.3
                Cass City                      18,610          0.9
            d.  Septic tanks* -                  ?              -
            e.  Known industrial -
                Sugar plant, Caro                ?
            f.  Direct precipitation** -        1,930          0.1
                         Total              2,043,340        100.0
        2.  Outputs -
            Lake outlet - Cass River        2,167,920
        3.  Net annual N loss - 124,580 pounds
     D.   Mean  Annual  Non-point Nutrient  Export by Subdrainage  Area:
         Tributary                       Ibs  P/mi2/yr   Ibs  N/mi2/yr   N/P Ratio
         Cass  River (A-2)                     66            3,111        47/1
**
Probably insignificant.
 See  Working  Paper  No. 1.

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                                 13
    E.  Yearly Loading Rates:

            In the following table, the existing phosphorus loading

        rates are compared to those proposed by Vollenweider (in press).

        Essentially, his "dangerous" rate is the rate at which the

        receiving water would become eutrophic or remain eutrophic;

        his "permissible" rate is that which would result in the

        receiving water remaining oligotrophic or becoming oligotrophic

        if morphometry permitted.  A mesotrophic rate would be consid-

        ered one between "dangerous" and "permissible".

            Note that Vollenweider's model  may not be applicable to

        water bodies with very short hydraulic retention times.

                                   Total Phosphorus       Total Nitrogen
        Units	Total   Accumulated    Total   Accumulated

        Ibs/acre/yr              300.0      loss*     10,216.7      loss*
        grams/m2/yr               33.63       -        1,145.1
        Vollenweider loading rates for phosphorus
         (g/m2/yr) based on surface area and mean
         outflow of Caro Reservoir:

            "Dangerous"  (eutrophic rate)       3.50
            "Permissible"  (oligotrophic rate)  1.75
* An apparent loss of some 13,000 Ibs of total  phosphorus and 125,000 Ibs
  of total nitrogen occurred during the sampling year.  While nitrogen loss
  can occur with certain conditions, losses of both nutrients at this mag-
  nitude makes it almost certain that a point source not accounted  for was
  involved.  In this case, the wastes from the beet-sugar processing plant
  are believed to be the unmeasured point source.

  Any discharges or seepage from the waste disposal ponds would enter the
  Cass River downstream from the Survey "inlet" station A-2 and would not
  be measured there.  However, the nutrient impact of such discharges would,
  in part at least, be measured at the outlet sampling station A-l  and thus
  result in the observed nutrient imbalance.

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                                 14
V.  LITERATURE REVIEWED

    Lehner, Richard A., 1972.   Treatment plant questionnaires  (Caro,
        Caro State Home, and Cass City STP's).  MI  Dept.  Publ.  Health,
        Lansing.

    Vollenweider,  Richard A. (in press).  Input-output models.   Schweiz,
        I.  Hydro!.

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                                  15





VI.  APPENDICES
                           APPENDIX A
                          LAKE RANKINGS

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