7281
no. 343
                                                 905R76010
            U.S. ENVIRONMENTAL PROTECTION AGENCY
                NATIONAL EUTROPHICATION SURVEY
                         WORKING PAPER SERIES
                                              REPORT
                                               ON
                                          VERSAILLES LAKE
                                           RIPLEY COUNIY
                                             INDIANA
                                           EPA REGION V
                                        WORKING PAPER No, 343
     CORVALLIS ENVIRONMENTAL RESEARCH LABORATORY - CORVALLIS, OREGON
                                 and
     ENVIRONMENTAL MONITORING & SUPPORT LABORATORY - LAS VEGAS, NEVADA
 •&G.P.O. 699-440

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                                   CONTENTS
                                                               Page
  Foreword                                                      i i
  List of Indiana Study Lakes                                   iv
  Lake and Drainage Area Map                                  v,  vi
  Sections
  I.   Conclusions                                                1
 II.   Lake and Drainage Basin Characteristics                    4
III.   Lake Water Quality Summary                                 5
 IV.   Nutrient Loadings                                          9
  V.   Literature Reviewed                                       14
 VI.   Appendices                                                15

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                          FOREWORD
    The National  Eutrophication Survey was initiated in 1972 in
response to an Administration commitment to investigate the nation-
wide threat of accelerated eutrophication to fresh water lakes and
reservoirs.

OBJECTIVES

    The Survey was designed to develop, in conjunction with state
environmental agencies, information on nutrient sources, concentrations,
and impact on selected freshwater lakes as a basis for formulating
comprehensive and coordinated national, regional, and state management
practices relating to point-source discharge reduction and non-point
source pollution abatement in lake watersheds.

ANALYTIC APPROACH

    The mathematical  and statistical procedures selected for the
Survey's eutrophication analysis are based on related concepts that:

        a.  A generalized representation or model relating
    sources, concentrations, and impacts can be constructed.

        b.  By applying measurements of relevant parameters
    associated with lake degradation, the generalized model
    can be transformed into an operational representation of
    a lake, its drainage basin, and related nutrients.

        c.  With such a transformation, an assessment of the
    potential for eutrophication control can be made.

LAKE ANALYSIS

    In this report, the first stage of evaluation of lake and water-
shed data collected from the study lake and its drainage basin is
documented.  The report is formatted to provide state environmental
agencies with specific information for basin planning {ง303(e)>, water
quality criteria/standards review {ง303(c)>, clean lakes (ง314(a,b)},
and water quality monitoring {ง106 and ง305(b)> activities mandated
by the Federal Water Pollution Control Act Amendments of 1972.

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    Beyond the single lake analysis, broader based correlations
between nutrient concentrations (and loading) and trophic condi-
tion are being made to advance the rationale and data base for
refinement of nutrient water quality criteria for the Nation's
fresh water lakes.  Likewise, multivariate evaluations for the
relationships between land use, nutrient export, and trophic
condition, by lake class or use, are being developed to assist
in the formulation of planning guidelines and policies by EPA
and to augment plans implementation by the states.

ACKNOWLEDGMENT

    The staff of the National Eutrophication Survey (Office of
Research & Development, U. S. Environmental  Protection Agency)
expresses sincere appreciation to the Indiana State Board of
Health for professional involvement, to the Indiana National
Guard for conducting the tributary sampling phase of the Survey,
and to those Indiana wastewater treatment plant operators who
provided effluent samples and flow data.

    The staff of the Division of Water Pollution Control, Indiana
State Board of Health, provided invaluable lake documentation and
counsel during the Survey, reviewed the preliminary reports, and
provided critiques most useful in the preparation of this Working
Paper series.

    Major General Alfred F. Ahner, Adjutant General  of Indiana,
and Project Officers Lt. Colonel Charles B.  Roberts (Retired)
and Colonel Robert L. Sharp, who directed the volunteer efforts
of the Indiana National Guardsmen, are also gratefully acknowledged
for their assistance to the Survey.

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                                    iv
                      NATIONAL EUTROPHICATION SURVEY

                               STUDY LAKES

                             STATE OF INDIANA
LAKE NAME

Bass
Cataract
Crooked
Dallas
Geist
Hamilton
Hovey
James
James
Long
Marsh
Mississinewa
Maxinkuckee
Monroe
Morse
01 in
Oliver
Pigeon
Syl van
Tippecanoe
Versailles
Wawassee
Webster
Westler
Whitewater
Winona
Witmer
COUNTY

Starke
Owen, Putnam
Steuben
LaGrange
Hamilton, Marion
Steuben
Posey
Kosciusko
Steuben
Steuben
Steuben
Grant, Miami, Wabash
Marshall
Brown, Monroe
Hamilton
LaGrange
LaGrange
Steuben
Noble
Kosciusko
Ripley
Kosciusko
Kosciusko
LaGrange
Union
Kosciusko
LaGrange

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                                    85' 14
                                                             85" 12
VERSAILLES LAKE

<8> Tributary Sampling Site
X Lake Sampling Site

 ?   .    V    .    ?Km.
 l      i      n
 o             ' Mi.
        Scale
                                                                 39'08—
                                                                 39" 04—

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                                      vi
I—39 20
    VERSAILLES  LAKE

 ฎ Tributary Sampling Site
 X Lake  Sampling Site
 ff Sewage Treatment  Facility
ป0 Drainage Area Boundary
 P   ....  5
                                                                         V>Km.
I—3910
I—3905
                  Ind.
                                                             )   2*

                                                          Map Location
      85 20
                                                                   85'05

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                             VERSAILLES LAKE
                             STORE! NO. 1850

I.  CONCLUSIONS
    A.  Trophic Condition:
            Survey data indicate that Versailles  Lake is  eutrophic.   It
        ranked twenty-third in overall  trophic quality when  the  27  Indiana
        lakes sampled in 1973 were compared using a  combination  of  six
        parameters*.   Twenty-four of the lakes had less median total  phos-
        phorus, 22 had less median dissolved phosphorus,  18  had  less
        median inorganic nitrogen, 19 had less mean  chlorophyll  a^,  and
        25 had greater mean Secchi disc transparency.  Near-depletion of
        dissolved oxygen with depth occurred at sampling  station 3  in
        August and October.
            Survey limnologists reported rooted aquatic vegetation  along
        about 20% of  the shoreline in August and  October  and some algae
        along the shore near station 1  in October.
    B.  Rate-Limiting Nutrient:
            The algal assay results indicate that Versailles Lake was
        phosphorus limited  at the time  the sample was  collected  (04/29/73).
        The lake data indicate phosphorus limitation at the  other sampling
        times as well.
    C.  Nutrient Controllability:
            1.  Point sources—The phosphorus contributions  of known  point
        sources amounted to 42.9% of the total  reaching Versailles  Lake
* See Appendix A.

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                             2
during the sampling year.   The  communities  of  Ba-
Osgood (2.7%),  and Napoleon (0.5%)  were  the sour
loads.  However, the non-point  phosphorus export
Creek was substantially higher  than would be exf
12).  It is possible that  additional  point  soun
ville or Osgood municipal  areas contributed sig
phosphorus load, the load  from  Napoleon  may hav
or both.
    The present phosphorus loading  of 44.42 g/r
that proposed by Vollenweider (Vollenweider am
a eutrophic loading (see page 13).   However, t
retention time of the lake probably is quite s
and Vollenweider's model may not apply.
    A 100% reduction in the phosphorus loads f
sources would lower the overall loading  rate t
Because Versailles Lake is phosphorus limited,
inputs should be minimized to the greatest  prc
slow the aging of this water body.
    2.  Non-point sources—About 57% of the t
to Versailles Lake came from non-point source
year.  Laughery Creek contributed 55.4%; howe
above, the phosphorus export rate of 58 kg/kr
higher than the rates of other  tributaries  i

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                             3
This may have been due in part to unknown point sources rather
than to non-point source inputs.   Other contributing gaged
tributaries were Cedar Creek, 0.7%; Unnamed Stream C-l, 0.5%;
and Falling Timber Creek, 0.3%.   The ungaged tributaries were
estimated to have contributed 0.2% of the overall  phosphorus
load.

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II.   LAKE AND DRAINAGE BASIN  CHARACTERISTICS1"

     A.   Lake Morphometry  :

         1.   Surface area:  0.93  kilometers2.

         2.   Mean depth:   Unknown.

         3.   Maximum depth:   9.1  meters.

         4.   Volume:   Unknown.

         5.   Mean hydraulic retention  time:  Unknown  but  probably  is quite
             short;  if the mean depth  is  equal  to  the maximum depth, the
             retention time would only be 24 days.

     B.   Tributary and Outlet:
         (See Appendix C  for  flow data)

         1.   Tributaries  -

                                               Drainage       Mean  flow
             Name                              area  (km2)*    (m3/sec)*

             Laughery Creek                        396.5           3.78
             Falling Timber Creek                   15.5           0.14
             Unnamed Stream C-l                       7.8           0.07
             Cedar Creek                             11.9           0.11
             Minor tributaries &
              immediate drainage  -                   4.1           0.02

                             Totals               435.8           4.12

         2.   Outlet -

             Laughery Creek                        436.7**         4.12

     C.   Precipitation***:

         1.   Year of sampling:  128.3  centimeters.

         2.   Mean annual:  105.4  centimeters.
 t Table of metric conversions—Appendix B.
 tt Winters, 1975.
 * For limits of accuracy,  see Working Paper No.  175,  "...Survey Methods,
   1973-1976".
 ** Includes area of lake.
 *** See Working Paper No.  175.

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                                       5
III.   LAKE WATER QUALITY SUMMARY
      Versailles Lake was sampled three times  during  the open-water
  season of 1973 by means of a pontoon-equipped  Huey  helicopter.   Each
  time,  samples for physical  and chemical  parameters  were collected
  from a number of depths at three stations  on the lake (see  map,  page
  v).   During each visit, a  single depth-integrated (4.6 m or near bottom
  to  surface) sample was composited from  the stations for phytoplankton
  identification and enumeration;  and  during the first visit,  a single
  18.9-liter depth-integrated sample was  composited for algal  assays.
  Also each time, a depth-integrated sample  was  collected from each
  of  the stations for chlorophyll  a_ analysis.  The maximum depths  sampled
  were 0.9 meters at station 1,  1.5 meters at  station 2,  and  4.6 meters
  at  station 3.
      The sampling results are presented  in  full  in Appendix  D and are
  summarized in the following table.

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B.  Biological characteristics:

    1.  Phytoplankton -
        Sampling
        Date

        04/29/73
        08/02/73
        10/10/73
    2.  Chlorophyll  a_ -

        Sampling
        Date

        04/29/73
        08/02/73
        10/10/73
Dominant
Genera

1.   Cryptgmonas sp.
2.   OscillatoTTa sp.
3.   Centric diatoms
4.   Synedra sp.
5.   Navicula sp.

            Total

1.   Stephanodiscus sp.
2.   Chroomonas sp.
3.   Flagellates
4.   Chlorogonium sp.
5.   Melosira sp.
    Other genera

            Total

1.   Oscillatoria sp.
2.   Melosira sp.
3.   Centric diatoms
4.   Merismopedia sp.
5.   Cryptomonas sฃ.
    Other genera

            Total
Station
Number

   1
   2
   3

   1
   2
   3

   1
   2
   3
Algal Units
per ml	
                                                           2,170
  9,794

 10,228
  3,133
  2,580
  1,843
  1,659
  4.607

 24,050
Chlorophyll
(yg/1)

     3.2
     9.9
    19.8
    64,
    29,
                                                              4.9
    30.
    41.
                                                             22.6

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                                 8
C.  Limiting Nutrient Study:

    1.  Autoclaved,  filtered,  and nutrient spiked -
        Spike (mg/1)

        Control
        0.050 P
        0.050 P  + 1.0 N
        1.0 N
Ortho P
Cone, (mq/1)

   0.050
   0.100
   0.100
   0.050
                                  Inorganic N    Maximum yield
                                  Cone,  (mg/1)    (mg/1-dry wt.)
                                      1.280
                                      1.280
                                      2.280
                                      2.280
19.7
22.6
29.0
21.8
    2.  Filtered, and nutrient spiked -
                         Ortho P
                         Cone,  (mg/1)
                 Inorganic N    Maximum yield
                 Cone, (mg/1)   (mg/1-dry wt.)
0.035
0.085
0.085
0.035
1.185
1.185
2.185
2.185
10.9
27.5
30.3
13.2
Spike (mg/1)

Control
0.050 P
0.050 P + 1.0 N
1.0 N
    3.  Discussion -

            The control  yields of the assay alga,  Selenastrum capri-

        cornutum, indicate that the potential  primary productivity

        of Versailles Lake was high at the time the sample was col-

        lected (04/29/73).  Both the autoclaved-filtered and filtered-

        only assays showed significant increases in yield with the

        addition of orthophosphorus alone, although smaller increases

        than would be expected.  Note that the addition of nitrogen

        alone did not result in significantly increased yields as

        compared to the control; hence, phosphorus limitation is indi-

        cated.

            The lake data indicate phosphorus limitation at all sam-

        pling times; i.e., the mean inorganic nitrogen/orthophosphorus

        ratios were 20/1 or greater.

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IV.   NUTRIENT LOADINGS
     (See Appendix E for data)
     For the determination  of nutrient  loadings,  the  Indiana  National
 Guard collected monthly near-surface grab  samples  from  each  of  the
 tributary sites indicated  on the map (page vi),  except  for the  high
 runoff months of January and February  when two samples  were  collected.
 Sampling was begun in June,  1973, and  was  completed  in  May,  1974.
     Through an interagency agreement,  stream flow  estimates  for the
 year of sampling and a "normalized" or average year  were  provided by
 the Indiana District Office  of the U.S.  Geological Survey for the
 tributary sites nearest the  lake.
     In this report, nutrient loads for sampled tributaries were
 determined by using a modification of  a  U.S.  Geological Survey  computer
 program for calculating stream loadings*.   Nutrient  loads shown are
 those measured minus point-source loads, if any.
     Nutrient loads for unsampled "minor  tributaries  and immediate
 drainage" ("ZZ" of U.S.G.S.) were estimated using  the means  of  the
 nutrient loads, in kg/km2/year,  at stations B-l, C-l, and D-l and
 multiplying the means by the ZZ  area in  km2.
     The operators of the Osgood  and Batesville wastewater treatment
 plants provided monthly effluent samples and corresponding flow data.
 The community of Napoleon  did  not participate in the Survey; nutrient
 loads from this source were  estimated  at 0.567 kg  P  and 3.401 kg
 N/capita/year,  and flows were  estimated at 0.3785  m3/capita/day.
 * See Working  Paper  No.  175.

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                                    10

    All  estimated phosphorus  loads were  reduced by 50% to adjust for a

phosphate detergent limitation  in effect in  Indiana since January, 1972.

    A.   Waste Sources:
        1.   Known municipal


        Name
                           t
Pop.
Served
Treatment
Mean Flow
(mVd)
        Batesville #1
                      }  4,000
        Batesville #2

        Osgood          1,500

        Napoleon          400**

        2.   Known industrial -  None
          tr.  filter    1,097.8

          act.  sludge   1,047.3

                          662.4
tr. filter
 + P-removal*
stab, pond      151.4
Receiving
Water

Little Laughery
 Creek
Little Laughery
 Creek
Branch of Plum
 Creek
Laughery Creek
t Treatment plant questionnaires.
* Begun in November, 1974.
** Anonymous, 1971.

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                                    11

    B.  Annual Total Phosphorus Loading - Average Year:

        1.  Inputs -

                                              kg P/          % of
            Source                            y_r	          total

            a.  Tributaries (non-point load) -

                Laughery Creek                22,875          55.4
                Falling Timber Creek             140           0.3
                Unnamed Stream C-l               195           0.5
                Cedar Creek                      285           0.7

            b.  Minor tributaries & immediate
                 drainage (non-point load) -      80           0.2

            c.  Known municipal STP's -

                Batesville #1                   9,920          24.0
                Batesville #2                   6,475          15.7
                Osgood                         1,100           2.7
                Napoleon                         225           0.5

            d.  Septic tanks*  -                   <5          <0.1

            e.  Known industrial - None

            f.  Direct precipitation** -          15          
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                                    12

    C.   Annual  Total  Nitrogen  Loading -  Average  Year:

        1.   Inputs -

                                              kg N/           % of
            Source                            y_r	          total

            a.   Tributaries (non-point load)  -

                Laughery Creek                302,320         89.9
                Falling Timber Creek            4,785          1.4
                Unnamed Stream C-l               3,690          1.1
                Cedar Creek                     7,445          2.2

            b.   Minor tributaries &  immediate
                 drainage (non-point load)  -     1,925          0.6

            c.   Known municipal  STP's -

                Batesville #1                    5,430          1.6
                Batesville #2                    4,215          1.3
                Osgood                          3,875          1.2
                Napoleon                        1,360          0.4

            d.   Septic tanks*  -                   175         <0.1

            e.   Known industrial  - None

            f.   Direct precipitation** -        1.005          0.3

                        Total                  336,225         100.0

        2.   Outputs -

            Lake outlet - Laughery Creek      353,070

        3.   Net annual N loss  - 16,845 kg.

    D.   Mean Annual Non-point  Nutrient Export by Subdrainage  Area:

        Tributary                             kg P/km2/yr    kg  N/km2/yr

        Laughery Creek                            58             762
        Falling Timber Creek                       9             309
        Unnamed Stream C-l                        25             473
        Cedar Creek                               24             626
* Estimate based on two camps and one park;  see Working Paper No.  175.
** See Working Paper No.  175.

-------
                                    13

    E.  Yearly Loads:

            In the following table, the existing phosphorus loadings
                               f
        are compared to those proposed by Vollenweider (Vollenweider

        and Dillon, 1974).  Essentially, his "dangerous"  loading is

        one at which the receiving water would become eutrophic or

        remain eutrophic; his "permissible" loading is that which

        would result in the receiving water remaining oligotrophic

        or becoming oligotrophic if morphometry permitted.   A meso-

        trophic loading would be considered one between "dangerous"

        and "permissible".

            Note that Vollenweider's model  may not be applicable to

        water bodies with short hydraulic retention times.

                                  Total Phosphorus       Total  Nitrogen
        	Total   Accumulated    Total    Accumulated

        grams/mVyr             44.42      13.56       361.5      loss*
        Vollenweider phosphorus loadings
         (g/m2/yr) based on surface area and mean
         outflow of Versailles Lake:

            "Dangerous"  (eutrophic loading)                 2.20
            "Permissible"  (oligotrophic loading)             1.10
* There was an apparent nitrogen loss during the sampling  year.   This  may
  have been due to nitrogen fixation in the lake, solubilization  of  pre-
  viously sedimented nitrogen,  recharge with nitrogen-rich ground water,
  unknown and unsampled point sources discharging directly to  the lake,
  or (probably) insufficient sampling in relation to  the short hydraulic
  retention time of the lake (see page 4).   Whatever  the cause, a similar
  nitrogen loss has occurred at Shagawa Lake, Minnesota, which has been
  intensively studied by EPA's  former National  Eutrophication  and Lake
  Restoration Branch (Malueg et al., 1975).

-------
                                    14

V.  LITERATURE REVIEWED

    Anonymous, 1971.   Inventory of municipal  waste facilities.   EPA
        Publ. OWP-1,  vol.  5,  Wash., DC.

    Grayer, John W.,  1973.  Treatment plant questionnaire  (Osgood  STP).
        Osgood Sanitation  Dept., Osgood.

    Plodder, Art, 1973. Treatment plant  questionnaires  (Batesville
        STP's).   Batesville.

    Malueg, Kenneth  W., D.  Phillips Larsen, Donald W.  Schults,  and
        Howard T. Mercier;  1975.  A six-year water, phosphorus,  and
        nitrogen budget for Shagawa Lake, Minnesota.   Jour.  Environ.
        Qua!., vol.  4,  no.  2, pp.  236-242.

    Vollenweider, R.  A., and  P.  J. Dillon,  1974.   The  application  of
        the phosphorus  loading concept to eutrophication research.
        Natl. Res. Council  of Canada Publ.  No.  13690,  Canada Centre
        for Inland Waters,  Burlington, Ontario.

    Winters, John, 1975.  Personal communication  (lake morphometry).
        IN Div.  of Water Poll. Contr., Indianapolis.

-------
VI.  APPENDICES
                                   APPENDIX A
                                  LAKE RANKINGS

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-------
    APPENDIX B
CONVERSION FACTORS

-------
                CONVERSION FACTORS




Hectares x 2.471 = acres



Kilometers x 0.6214 = miles



Meters x 3.281 = feet

                         -4
Cubic meters x 8.107 x 10   = acre/feet


Square kilometers x 0.3861 = square miles


Cubic meters/sec x 35.315 = cubic feet/sec


Centimeters x 0.3937 = inches


Kilograms x 2.205 = pounds



Kilograms/square kilometer x 5.711 = Ibs/square mile

-------
    APPENDIX C
TRIBUTARY FLOW DATA

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        APPENDIX D
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-------
  APPENDIX E
TRIBUTARY DATA

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