EPA 903/9-78-009
SUMMARY OF OHIO RIVER FISHERY
          SURVEYS,  1968-76
                             Region III Library
                          Environmental Protection Agency
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             Surveillance and Analysis Division
                     Region III
            U. S. Environmental Protection Agency
             Philadelphia, Pennsylvania 19106
                                    EPA Report Collection
                                   Information Resource Center
                                     US EPA Region 3
                                    Philadelphia, PA 19107

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Regional Center for b m ironmetit.il Ii
             USEP<\ Kcyionlll
                1650ArcliSt
           Philadelphia, PA 19103

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                                                   EPA
EPA 903/9-78-009                                   June  1978
       Summary of Ohio River  Fishery  Surveys,  1968-76
                             by
                      H. Ronald Preston
            U. S. Environmental Protection Agency
        Wheeling Field Office, Wheeling, West Virginia
                            and
                       Glenn E. White
        Ohio River Valley Water Sanitation Commission
                      Cincinnati, Ohio
                                                        PA  19107
              Surveillance and Analysis Division
                          Region III
            U. S. Environmental Protection Agency
               Philadelphia, Pennsylvania 19106

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                              DISCLAIMER
     This report has been reviewed by the Surveillance arid Analysis
Division, Region III, U..S. Environmental Protection Agency and the
Ohio River Valley Water Sanitation Commission.  Approval does not
signify that the contents necessarily reflect the views and policies
of the U. S. Environmental Protection Agency nor ORSANCO.
                                   11

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                               FOREWARD
     Evaluations of water pollution abatement programs must begin with
an adequate inventory of the biological resources affected.  Future
trend analysis is dependent upon basic ambient biomonitoring information.
The collection of such biomonitoring data in a large river can be complex
and difficult to obtain and may be limited to certain biological com-
munities .

     The primary mission of this biomonitoring activity is to provide a
baseline of data describing the kinds of fish and their relative abundance
in the Ohio River.

     This  report provides a valuable contribution to the knowledge of the
Ohio River and its aquatic life.

                                         Jack J.  Schramm
                                         Regional Administrator
                                         Region III
                                  111

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                                 ABSTRACT
     The fish life community of the Ohio River was sampled annually 1968-1970
and 1975-1976 at selected locations.  The objective of these investigations
was to establish a base line of data which can be used to compare past studies
and to identify water quality trends.  The samples were collected from lock
chambers of navigation structures located throughout the length of the river.
In general, the results of these studies documented the existence of a more
abundant and desirable fish population than observed during investigations
performed in the 1950's.  Shifts in species domination and composition were
observed in sections of the river and reflected water quality conditions.   A
total of 181,000 fish weighing 11,569 kilograms, composed of 82 species, was
collected in this study.

     For comparative purposes, the Ohio River fish fauna was separated into
related categories which provided a means of characterizing differences
between sections of the river.  The upper Ohio River fish fauna was indicative
of improving water quality while the lower Ohio River fauna in general, reflected
stable water quality conditions.  The most notable shifts in species composition
occurred in river sections affected by the metropolitan complexes and these
populations were composed of less desirable fishes.

     Pollution abatement programs have resulted in improved water quality
conditions in the Ohio River and the fish populations have responded.  The
construction of the higher navigation dams created additional favorable shore-
line habitats for several recreationally valuable fishes.  These factors
contributed significantly to the increased recreational use of the river.
                                     IV

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                             CONTENTS
Disclaimer	 ii
Forward	 iii
Abstract	 iv
Contents	 v
Figures	 vi
Tables	 vii
     Introduction	 1
     The Ohio River	 2
     Historical Review	 3
     Study Locations and Methods	 4
     Results	 5
     Conclusions	 8
     Literature Cited	10
     Appendix	11

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                                 FIGURES




Number                                                               Page




 1   Map, Ohio River and station locations	   A-2




 2   Fish biomass estimates for 100 mile river segments	   A-3
                                       VI

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                             TABLES

Number                                                           Page

 1   Ohio River fish sampling locations	  A-4

 2   Distribution of fishes collected in Ohio River 	  A-5

 3   Relative abundance of Ohio River fishes 	  A-14

 4   Total weights and weight of fish per unit area,
       station by station, year by year 	  A-18

 5   Ohio River fish category distribution at primary
       locations, year by year 	  A-19

 6   Comparison,  by percent, of fish categories at primary
       locations, 1968-1976 	  A-22

 7   Fish biomass estimates [Kg/ha) for 100 mile river
       segments  	  A-23

 8   Comparison of most abundant Ohio River fishes,
       1957-59 vs. 1968-76  	  A-24

 9   Number of fish species and number of fishes collected
       at primary locations, year by year	  A-25
                                   VII

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                               INTRODUCTION

     The assessment of water quality through examination of resident aquatic
life communities has been employed extensively by pollution investigators
during the past several decades.   The science of water pollution biology is
not that recent, as its conception and practical application occurred in the
early 1900's.  However, extensive use of biological data in water quality
evaluations was not employed with much understanding until the 1950's.  The
degradation of water quality brought about by socioeconomic expansion in the
Twentieth Century resulted in state and federal legislation to slow down and
stop the environmental deterioration.  Comprehensive laboratory and field
water quality investigations, including biological studies, have been a result
of these legislations.  In performing these studies, aquatic scientists have
shown the relevancy of aquatic life observations in understanding causes and
impacts of environmental alterations.  Biological assessments and evaluations
have become basic tools in predicting environmental conditions that may result
from varying degrees of pollution control.  The current concerns for toxic
substances in the environment has further substantiated the relevancy of
aquatic life data in evaluating water quality, its significance and value to
man.

     Biological water quality evaluations in streams have primarily concen-
trated on the collection of benthic organisms.  The analyses of benthic
community structures and their ecological roles provide a basis for determining
pollutional impacts.

     Dependent upon study scope and objectives, other communities, i.e.,
plankton and fish have also been utilized for these evaluations.  Fish occupy
a unique position among aquatic communities.  Their populations, diversity,
age, reproduction, and community structure reflect long term water quality
trends.  Public interests in, and direct beneficial uses of fishery resources
(recreational and commercial), place the fish community in a position of high
recognition to society.  It has been pointed out by aquatic scientists that
because of these uses fish parameters can be used as a direct economic measure
of the effects of pollution.

     Beginning in the late 1960's cooperative fishery resource studies were
initiated for the entire length of the Ohio River.  The cooperating agencies
involved were the Pennsylvania Fish Commission, Pennsylvania Department of
Environmental Resources, West Virginia Department of Natural Resources, Ohio
Department of Natural Resources,  Kentucky Department of Fish and Wildlife,
Kentucky Department of Environmental Protection, Indiana Stream Pollution
Control Board, Illinois Environmental Protection Agency, Ohio River Valley
Water Sanitation Commission,  U. S.  Army Corps of Engineers, U.  S.  Fish and
Wildlife Service and U. S. Environmental Protection Agency.  In addition,

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personnel from universities and scientific institutions participated in
special aspects of the studies.  Institutions represented were The Pennsyl-
vania State University, University of Pittsburgh, The Ohio State University,
Marshall University, University of Louisville, Auburn University and The
Smithsonian Institute.

     The overall purpose of these studies is to evaluate past, present and
future water quality conditions of the Ohio River.  Specifically, the data
obtained will provide:  information describing trends developing in the
composition of the fishery resource and comparative data to evaluate changes
from the study conducted in 1957-59 by ORSANCO-University of Louisville;
information relative to determining sections of the Ohio River most severely
impacted by pollution; data on the quality and quantity of Ohio River fishes,
including those of recreational and commercial value; and a baseline of data
to evaluate effectiveness of pollution abatement programs.

THE OHIO RIVER

     The basin of the Ohio River drains an area of 528,127 square kilometers
(203,910 square miles) and is formed by the confluence of the Allegheny River
from the north and the Monongahela River from the south at Pittsburgh, Penn-
sylvania.  From Pittsburgh it flows in a generally southwest direction,
forming the western border of West Virginia, the northern border of Kentucky,
and the southern borders of Ohio, Indiana and Illinois.  It meets the Missis-
sippi River at Cairo, Illinois 1,580 kilometers (981 miles) downstream of
Pittsburgh.  There are approximately 40,500 hectares (100,000 acres) of
surface area in the Ohio River.

     The Ohio is the eleventh largest river in the United States in length
and has the greatest discharge of the six Mississippi tributaries.  The
average flows in cubic feet per second for the period 1946-1975 were:  South
Heights, Pennsylvania, mile point 15.2, low of 10,000 cfs in September and
high of 70,000 cfs in March; Cincinnati, Ohio, mile point 462.4, low of
30,000 cfs in September and high of 200,000 cfs in March; and Evansville,
Indiana, mile point 791.5, low of 40,000 cfs in September and a high of
300,000 cfs in March.

     The Ohio has relatively steep banks and flows in a narrow valley for
most of its length, therefore having few shallow wetland areas which are
conducive to fish reproduction.  It has a gravelly bottom for most of its
upper reaches and is dotted with about 130 islands.  The U. S. Corps of
Engineers maintains a 3 meters (nine feet) channel for navigation; however,
the depth varies up to 14 meters(45 feet) in areas upstream of the navi-
gation dams.  Geologically, the Ohio River basin is composed principally
of sedimentary rock which varies from siltstone and shales to limestone and
sandstone.  The prototype of the Ohio was the Teays River system which existed
in pre-glacial times.  While there are few natural lakes in the Ohio River

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 drainage basin, many of the tributary streams have been impounded for flood
 control, water supplies and recreational use.   In 1824 the Corps of Engineers
 began modification of the river through rock removal and construction of dikes.
 Eventually this lead to 46 wicket dams, completed by 1929  (Butz, et al., 1974).
 In the mid 1950's work began on the present day 19 higher  lift structures that
 have turned the Ohio into a series of slack water navigation lakes and  have
 effected changes in aquatic life habitats.

 HISTORICAL REVIEW

     There have been numerous studies of the fishes of the Ohio River,  gener-
 ally localized in their scope.  Many were designed to study fish populations
 in specific habitats or areas affected by specific sources of pollution.

     Lachner  (1956) summarized early Ohio River fishery studies by C. A.
 LeSueur and J. P.  Kirkland.  C. S. Rafinesque (1820) reported 113 species of
 fish in the Ohio River.  While there are some inaccuracies in this data, it
 shows the well developed fish fauna before the Ohio became a great industrial
 waterway.  Jackson (1962) reviewed early fish studies of the Ohio River and
 described habitat changes of the river and the general effects of these changes
 on the fish fauna.   Trautman (1963) summarized early travels down the Ohio and
 reported that one observer in 1972-73 saw a great abundance of fishes.
 Trautman also described the changing land use and its effect on the fish life.

     After about 1900, increased siltation caused changes in the fish popu-
 lations with bullhead catfish, goldeye, skipjack herring, gizzard shad,  and
 spotted bass increasing in numbers.  Through the late 1800's and the early
 1900's the greatest effect on fish populations took place.  This was due in
 large part to land use and subsequent runoff from deforestation, agriculture
 and mining.  In the 1920's mining took the largest toll and in the 1930's it
 was industrial expansion that contributed most to water degradation.

     In 1933,  an observer in Ashland, Kentucky reported a fish kill which
 took better than two days to pass and completely covered the river (Jackson
 1962).   By mid-century, 24 species of fish,  found in earlier studies,  were
 reported to be either missing in the upper river or in very low numbers
 (Lachner 1956) .

     Industrial  discharges have also affected aquatic populations in recent
 tines.   In the study by Krumholz and Minckley (1964) referring to the 1957-59
data,  the authors  stated:   "on the basis of these findings there is little
doubt  that the abatement of pollution in the upper Ohio River during the
steel  strike of 1959 provided a marked change in water quality that led  to a
reinvasion of the  main channel of the river by fishes from nearby unpolluted
waters.   There was  a marked resurgence in the fish populations of the river,
both in species  composition and in numbers of individuals."

     By 1968,  with  the reduced effects of acid mine drainage,  urban and
 industrial wastes  and siltation,subtle changes in the fish populations were

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observed (Preston 1969).  Carp and bullhead catfish were predominant in the
upper river.  Other species found included channel catfish,  sunfishes,  fresh-
water drum and shiners.   The sunfishes increased in the middle third of the
river and the commercial forms were more abundant in the lower half of the
river.

    The modernization of the navigation structures also affected aquatic
populations in the river.  The construction of higher dams has created
additional shoreline habitats which contributed to the increased numbers
of several fish species, especially those in the sunfish family.

     The recent report prepared for the National Commission on Water Quality
by Dames and Moore, Inc. (1975) outlines an excellent historical review of
fishery investigations conducted since the early 1800's.  Further, the Dames
and Moore report describes past water quality problems and future trends and
impacts of pollution abatement programs.

STUDY LOCATIONS AND METHODS

     During the years 1968, 1969, 1970, 1975, and 1976 fishery resource studies
on the Ohio River were conducted at 20 different lock chambers operated by the
U. S. Army Corps of Engineers.  Table 1 lists the sampling locations and year
sampled.  Figure 1 is a map of the Ohio River with primary stations located
and their river mile point.  Due to the construction of new higher-lift dams,
a portion of these sampling locations have been removed and the sites inundated.
The upper river was sampled in 1977, but, because data is lacking for the lower
river, comparative analysis is not possible and therefore these data are not
discussed in this report.  During the study period, 59 separate fish samplings
were conducted.  However, only eight locations (considered primary stations)
were sampled for each of the five year studies for a total of 40 samples.  The
data from these primary sites are compared in this report.  The lower river
stations (Locks and Dams No. 50, No. 52, Smithland Locks and Dam) are close
together and are treated as one river section.  The navigation higher lift
structures have two lock chambers, 110 X 600 feet and 110 X 1200 feet.  The
smaller auxiliary chamber was selected for sampling because of its size and
it presented a minimum of interference with barge traffic.  Surface areas of
the locks varied from 0.2025 to 1.2150 hectares and 5 of the ten sites are
0.6075 hectares.

     Dashields Lock and Dam was selected because it is located below the
Pittsburgh and upper river metropolitan area which is heavily indusrialized;
Pike Island Lock and Dam is situated at Wheeling, a partial recovery zone
from upstream industry; Belleville Lock and Dam is located just below
Parkersburg, West Virginia, and was selected because it is situated below
a series of upstream industries and is above the influence of the Kanawha
River; Gallipolis Lock and Dam was chosen since it is immediately downstream
of the Kanawha.  Meldahl Lock and Dam is above Cincinnati, and Markland Lock
and Dam is below Cincinnati.  These locations show the effects of urban

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pollution.  Channelton Lock and Dam wa^, chosen because it is below the
Louisville industrial complex; and the last three sites, Lock and Dam
No. 50, 52, and Smithland Lock and Dam (a distance of 62 miles) were
combined for reflecting fish populations in the lower end of the Ohio
River.

     Fish population studies are difficult to conduct in large deep rivers.
Several methods are available, such as nets, trawls, electrofishing, and
ichthyocides.   All of these methods have their advantages and disadvantages.
Rotenone, a toxicant, is relatively non-selective and is semi-effective in
sampling deeper waters.  However, certain requirements must be met in its
use.  In a river situation with lock chambers at navigable dams, rotenone
has the advantage of sampling a confined area where it is concentrated and
cannot be diluted.

     The U. S. Army Corps of Engineers personnel were notified of the planned
study well in advance and localized schedules were arranged.  The day prior
to the lock study, the lock personnel were again contacted and requested to
leave the lower gates of the auxiliary chamber open for at least 2 hours prior
to entry and preferably from midnight on if possible.  At eight o'clock in the
morning, the crews, usually 5 boats and 12-15 persons, entered the lock on
the downriver side.  The gates were closed and the water level maintained at
the lower pool level throughout the sampling period.  On rare occasions there
were some problems with leaky upper gates and the lock chamber would gradually
fill.  Five percent rotenone emulsion was applied with a surface pump and a
submerged, perforated 25 foot hose, to a concentration of 0.5 to 1 part per
mi LI ion.

     Minnows and shad began to surface almost immediately since they are the
more rotenone sensitive species.  Last to come up are the hardier species such
as carp, catfish and gar.   Fish were dipped up in long handled nets until
there was a definite tapering off of fish surfacing.  Then the lock personnel
were instructed to fill the lock chamber 2-3 feet.  This tended to wash larger
specimens from the bottom that either surfaced and sunk or did not surface the
first time.  In most instances the fish collection was finished by 12:00 noon.
Following the collection,  the fish were sorted, identified, measured and weighed.
AlL fish were placed in length classes of 3 centimeter increments and weighted
in grams.  The smaller fish were preserved in 10 percent formalin for later
laboratory processing.   In the sampling sessions, professional fishery biologists
from state and federal agencies assisted in the operation.

RESULTS

     A total of 82 species of fish were identified from the 1968-77 collections.
Table 2 lists the fishes collected and their Ohio River distribution.  Table 3
lists these fishes by common name, along with relative abundance estimates.
For gross comparative purposes of species distribution, the Ohio River was
subdivided into three sections and the subjective relative abundance designations
were based on frequency of occurrence in the collections, distribution patterns
(Table 2) and known geographic range of these species.  Thirty four of the

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82 species are distributed throughout the length of the Ohio River.   These
species are identified by an asterisk in Table 2.   With few exceptions,
distinct distribution patterns for the remaining species cannot be determined
with data that is now available.   The exceptions include the blue catfish,
threadfin shad, and paddlefish which are limited to the lower Ohio River and
the white catfish which has only been collected in the upper river.   The
Dames and Moore (1975) review indicates that the lake sturgeon, Acipenser
fulvescens, and the striped shiner Notropis chrysocephalus are still found in
the Ohio River.  These two species were not reported in the 1957-59 study nor
in the 1968-76 surveys.  However, this statement may have been based on
commercial fisherman catches or private industry studies referred to in the
report.

     Eight species were collected in this study that were not reported in the
1957-59 study.  These are alewife, Alosa pseudoharengus; northern pike,  Esox
lucius;  bigeye shiner, Notropis boops; pugnose minnow, Notropis emiliae;
white catfish, Ictalurus catus; striped bass, Morone saxatilis and channel
darter,  Percina copelandi.   There were several species listed in the 1957-59
study that were not collected in this study.  However, the earlier study
report included many Ohio River tributary collections, whereas the 1968-76
collections were confined to the mainstem Ohio River.

     The black bullhead was rarely collected in the 1968-76 collections;
whereas, it was listed in the 1957-59 collections.  The field biologists
participating in the 1968-76 collections recognized the lack of definitive
characteristics in the bullhead  (yellow excepted)  and submitted samples to
several experts (personal communications, Reeve Bailey, Branley Branson,
Ted Cavender and Milton Trautman).  These taxonomists agreed that all samples
were brown bullheads with intermediate characteristics between black and
brown bullheads.

     A review of the individual sample tabulations suggest some notable
observations concerning shifts in species distributions.  For the period
of study, species that appear to be extending their range from downstream
to upstream include the sauger and freshwater drum.  Species that appear to
be increasing in abundance in the upper 100 miles of the river include the
channel catfish, skipjack herring and spotted bass.  Also, individual sample
collections indicate that the paddlefish and buffalofish may be extending
their range and/or increasing from the lower Ohio into the middle section
of the river.  Recreationally valuable fishes, i.e. largemouth bass, spotted
bass, white and black crappies, sauger, and channel catfish have occurred
in the samples with regularity.  However, fisherman catch reports (corre-
spondence with state agencies) show that increasingly  larger numbers of bass
and sauger have been taken in recent years, indicating an increasing abundance
of these fishes.

     The total number of species collected at the eight primary stations  in
the 1968-76 period were:  Dashields, 31; Pike Island,  38; Belleville, 48;
Gallipolis, 37; Meldahl, 41; Markland 36; Cannelton, 45; and the farthest
downstream station  (No. 40, No.  52, Smithland), 46  (see Table 9).

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Biomass Distribution and Trends

     The 40 samples collected from the eight primary stations during 1968
through 1976 yielded a combined total of 181,000 fish weighing 11,569
kilograms (25,452) pounds.  Total weight of fishes collected annually are
listed below.

               Year                        Total Kilograms (pounds)

               1968                            2390   (5258)
               1969                            1985   (4367)
               1970                            1549   (3408)
               1975                            2211   (4864)
               1976                            3434   (7555)

     For comparative purposes, the fish biomass collected at each location
was converted to a weight per unit area sampled (kilograms/hectare) and is
given in Table 4.   These data show a greater fish biomass in all the down-
stream stations (mile points 436 to 939) in both subperiods of the study
(1968-70 and 1975-76) .

     Table 5 is a station by station comparison of fish categories and their
relative abundance (kg/ha) that were obtained from the Ohio River fish samples
conducted in each of the sample years.  The categories were arbitrarily created
to best describe the Ohio River fish association.   The criteria used to develop
these categories were based on phylogentic relationships, ecologic and eco-
nomic factors.  Table 6 compares these fish categories (on a percent basis,
kg/ha) between the primary stations for the study period 1968-1976.

     These categories are:

           Category                         Forms included

           Forage A                All minnows, shiners and chubs
           Forage B                Shad and herrings
           Sport A                 All sunfishes and basses
           Sport B                 Walleye, sauger and perches
           Commercial              Channel catfish, blue catfish,
                                   buffalofishes,  freshwater drum
           Rough                   Carp, bullhead catfish and suckers
           Miscellaneous           All others

     Table 7 is a comparison of fishery biomass data obtained from the
1957-59 aquatic-life study (Krumholz, et al.  1962) and the 1968-76 data.
The 1968-76 data have been rearranged from other presentations in this
report to fit the river section designations used in the 1957-59 study.
Figure 2 graphically shows these data and reflects the fish biomass trend
in the Ohio River.  The 1968-76 data estimates compared to the 1957-59 data

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show less of a biomass change in the river segments 100-300 miles and 700-
800 miles than in the other river segments.   The greater biomass changes
between these studies occurred in river segment 0-100 miles, segment 300-700
miles and segment 800 miles to the mouth of the river (981 miles).

     Table 8 is a species abundance comparison between the 1957-59  study
and the 1968-76 study based on number and weight rankings.  The abundance,
by number, comparison reflected little change in the order of species
dominance.  A commercially important form, the blue catfish did not appear
in the 1968-76 rankings (number of weight).   The abundance, by weight, of
the first four fish did not change in the two study periods.  The rankings
show a change in the positions 6 through 10.   The disappearance of the skip-
jack from 1968-76 rankings is a puzzling phenomenon.  The buffalofishes (big-
mouth, smallmouth, and black) have displaced the skipjack, flathead catfish
and blue catfish in the abundance, by weight, in 1968-76 data.

CONCLUSIONS

     Because of the manner by which the data collected in the ORSANCO-
University of Louisville study of 1957-59 was composited, it is difficult
to outline specific comparisons.  However, certain generalized conclusions
can be drawn:

    •The fish biomass estimates for the 1968-1976 period were greater than
the estimates derived from the 1957-59 study.  Throughout the length of the
river there was approximately five times greater abundance of fish during
the recent study than there were in the earlier study.  The sections of the
river with the most significant increases occurred in the sections  0-100 miles;
500-600 miles and 900-981.

    •The general species composition was about the same and little difference^
were observed in the predominant species of both studies.  Certain forms
(basses, sauger, drum) appeared to be extending their upstream distribution
in the more recent study.


     Differences that were observed between the 1968-70 subperiod and the
1975-76 subperiod were:

    •The data indicated a decrease in total biomass in the upstream portion
of the river (0-280 miles) from the 1968-70 subperiod to the 1975-76 subperiod;
an exception being the Belleville (mile point 204) samples.  Although no major
shift of species occurrence was detectable,  it was apparent that these decreases
were due almost entirely to a reduction in the numbers of carp and bullhead
catfish in the samples.  It was assumed that there has been a reduction in
biodegradable organic loads to the upper Ohio River in recent years due to
pollution abatement pro'grams and this may have produced an environment less
conducive to domination by these species.

    •Concurrent with the decline of the "rough" species, the "commercially"
valuable fishes, i.e. channel catfish and freshwater drum, increased in the

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1975-76 subperiod over that observed in the 1968-70 subperiod in the upper
portion of the river.

    •In the upstream third of the Ohio River, minnows and shiners appeared in
greater numbers than at downstream stations.  Many of the same species occurred
in the lower river but in fewer numbers.

    •Forage B and Commercial categories increased from upstream to downstream.

    'The commercially valuable fishes increased significantly in the fishery
composition at the Gallipolis location and were a dominant form in the down-
stiearn half of the river.

    •The Sport A and B categories were less abundant in the upper Ohio River
(0-200 miles) than in the downstream sections.

    •The proportion of the biomass contributed by "rough fish" decreased in
th-,.  downstream samples.

    •The miscellaneous forms, as a composite group, were more numerous in
the middle and lower sections of the river.  This correlated with increased
species diversity in the downstream sections.

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                        LITERATURE CITED
1.   Butz,  B.  P.,  D.  R.  Schregardus,  B.  A.  Lewis, A.  J.  Polichstro, and
    J. J.  Reisa,  Jr.  1974.   Ohio River Cooling Water Study, 905/9-74-004.
    U. S.  Environmental Protection Agency,  Washington,  D.  C.

2.   Dames  and Moore,  Inc.   1975.  Water Pollution Control  Act of 1972.
    Regional  Impacts, Ohio  River Basin, Vol.  I & II, PB 249 680-01 f, 02.
    The National  Commission on Water Quality,  The National Technical
    Information Service, U. S. Department  of Commerce,  Springfield,
    Virginia.

3.   Jackson,  D. F.  1962.   Historical notes on fish fauna,  pp. 1-19.
    In:  Aquatic-Life Resources of the Ohio River.  Ohio River Valley
    Water Sanitation Commission, Cincinnati,  Ohio.

4.   Krumholz, L.  A.,  J. R.  Charles and W.  L.  Minckley.   1962.  The fish
    population of the Ohio  River,  pp.  49-89.   In:  Aquatic-Life
    Resources of the Ohio River.  Ohio River Valley Water Sanitation
    Commission, Cincinnati, Ohio.

5.   Krumholz, L.  A.,and W.  L. Minckley.  1964.  Changes in the fish
    populations in the Upper Ohio River following temporary pollution
    abatement.  Trans,  of Am. Fish Soc., 93 (]): 1-5.

6.   Lachner,  E. A.  1956.   The changing fish fauna of the Upper Ohio
    Basin,  pp. 64-78.   In:  Man and the Waters of the Upper Ohio Basin.
    Special Publication Number 1, Pymatuning Laboratory of Field Biology,
    University of Pittsburgh, Pittsburgh,  Pennsylvania.

7.   Preston,  H. R.  1969.   Fishery Composition Studies - Ohio River Basin.
    FWPCA Presentations.  September 10, 1969.   Minutes of the Engineering
    Committee, Ohio River Valley Water Sanitation Commission, Cincinnati,
    Ohio.

8.   Rafinesque, C. S.  1820.  Icthyologia Ohiensis.  American Environmental
    Studies.   1970.  Reprinted by Arno Press,  New York.

9.   Trautman, M.   B.  1963.   Fishes of Ohio.  The Ohio State University
    Press, Columbus.
                                  10

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APPENDIX
   11

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                                           A-3
  1300
  1200
  1100
  1000
   900
   800
   700





-------
                                     A-4
                  Table 1.  Ohio River Fish Sampling Locations

Lock & Dam Locations, Mile Point     1968   1969   1970   1975.   1976   1977
* Dashield
Montgomery
New Cumberland
* Pike Island
Hannibal
No. 15
* Belleville
No. 23
at
Gallipolis
Greenup
* Meldahl
Mar kl and
McAlpine
No. h3
Channelton
Newburgh
Uniontown
(No. 50
*( Smithland
( No. 52
13
31
^
8k
126
129
20U
231
279
3M
1+36
531
607
633
720
778
8U6
876
918
938
.3
• 7
.*
.3
.U
.1
.0
.0
.2
.0
.4
• 5
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.2
.7
.7
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.8
,5
,9
X
X
X
X

X
X
X
X
X
X
X
X
X
X
X

X


X
X
X
X

X
X

X
X
X
X
X
X
X
X
X
X


X
X
X
X

X
X

X
X
X
X
X
X
X
X

X


XXX


XXX
X X

XXX

XXX

X X
X X
X

X X



X
X
 *Primary evaluation stations.

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-------
                                 A-14



         Table  3.  Relative Abundance of Ohio River Fishes
  Species



  Silver lamprey



  Paddlefish
  Longnose gar
  Shortnose gar



  Bowfin
  American  eel
*
   Skipjack herring
  Alevife



  Gizzard  shad



  Threadfin  shad



  Goldeye



  Mooneye



  Northern pike



  Goldfish
*  „
   Carp
  Stoneroller



* Silver chub



  Golden shiner



* Emerald  shiner
,YT

  River shiner
  Bigeye  shiner



  Ghost shiner
  A  - Abundant   C  -  Common  0 - Occasional  R  - Rare


  * Distributed throughout Ohio River
Upper
R
R
0
R
R
0
C
R
A
R
R
R
R
0
A
R
0
0
A
C
R
0
Middle
R
0
C
R
R
0
C
R
A
0
0
0
R
0
A
R
0
R
A
C
R
0
Lower
R
C
C
0
R
0
C
R
A
0
0
0
R
0
A
R
0
R
A
0
R
R

-------
                           A-15
       Table 3.  Relative Abundance of Ohio River Fishes
                        (continued)
Species

Common shiner

Pugnose shiner

Rosyface shiner

Spotfin shiner

Sand shiner

Mimic shiner

Steelcolor shiner

Suckermouth minnow

Bluntnose minnow

Fathead minnow

Creek chub

River carpsucker

Quillback carpsucker

Highfin carpsucker

White sucker

Smallmouth buffalo

Bigmouth buffalo

Black buffalo

Spotted sucker

River redhorse sucker

Golden redhorse sucker

Shorthead redhorse sucker R

Black redhorse sucker
Upper
0
R
R
C
A
A
0
R
A
R
R
C
C
0
0
R
R
R
0
0
C
;r R
C
Middle
0
R
R
C
C
C
R
R
A
0
R
C
C
C
0
C
C
0
0
0
C
R
0
Lower
0
R
R
0
0
0
R
R
C
0
R
C
C
C
0
C
C
0
0
0
C
R
R
*Distributed throughout Ohio River.

-------
                             A-16
       Table 3•   Relative Abundance of Ohio River Fishes
                        (continued)


*


*
*
*




*


*

*

*
*
*

*
Species
Blue catfish
Channel catfish
White catfish
Black bullhead catfish
Yellow bullhead catfish
Brown bullhead catfish
Flat head catfish
Stonecat
Trout-Perch
Banded killifish
Pirate perch
White bass
Striped bass
Yellow bass
Rock bass
Smallmouth bass

Spotted bass
Largemouth bass
Warmouth sunfish
Green sunfish
Pumpkinseed sunfish
Orangespotted sunfish
Upper
R
A
C
R
C
A
C
R
R
0
R
0
R
R
R
0

C
C
R
C
0
0
Middle
0
A
0
R
C
A
C
R
R
R
R
0
R
R
R
0

C
C
0
0
0
R
Lower
C
A
R
R
C
C
C
R
0
R
R
0
R
0
R
0

C
C
0
0
0
R
*Distributed throughout Ohio River.

-------
                              A-17


        Table  3.   Relative Abundance of Ohio River Fishes
                         (contined)
Species

Bluegill sunfish

Longear sunfish

Redear sunfish

White crappie

Black crappie

Johnny darter

Yellow perch

Log perch

Channel darter

Blackside darter

Sauger

Walleye

Freshwater drum

Brook silverside
Upper
C
0
0
C
C
R
0
0
R
R
0
0
C
R
Middle
C
0
0
C
C
R
R
0
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0
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Lower
C
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0
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C
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R
0
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0
A
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*Distributed throughout Ohio River.

-------
                                                                        A-18
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-------
                      A-19

Table  S  Ohio River Fish Category Distribution
            at Selected Locations.

              KILOGRAMS/HECTARE
Sample
Location
DASHIELDS
Forage A
Forage B
Sport A
Sport B
Commerc ial
Rough
Miscellaneous
PIKE ISLAND
Forage A
For B
Sport A
Sport B
Commercial
Rough
Misc .
BELLEVILLE
For A
For B
Sport A
Sport B
Comm.
Rough
Misc .
GALLIPOLIS
For A
For B
1968
20.99
6.1*7
3.21
3.60
529.58
0.05
2.09
13-1*1
9-71
2.93
ll*.86
251.90
0.38
2.93
9.53
0.1*8
33.61
133. 51*
1.1*2
5.76
39-^2
1969
17. lU
16.1*9
1.63
2.72
2.22
l*93.lU
l*.5l*
9-25
21.53
5-15
10.62
190.62
0.51
27.65
7.52
3.7l*
32.31
38.88
0.31
3-1*0
21.0U
1970
9.1*8
20.1*1*
0.61*
10.81
277-93
3-70
30.65
1*8.61*
6.09
11.19
361*. 15
U.67
10.39
25.19
15.00
15.1*9
97.07
5.10
2.00
11.77
1975
11*6.1*7
116. Jk
O.Ik
1.09
39-1*1
1*1*. 20
7-70
3.1*9
36.97
17.27
O.Ik
21.00
122.21*
2.50
1.91
89.58
17.09
8.07
51.13
98.52
6.02
0.55
81.95
1976
8.15
1.78
31.56
22U.25
1.1*8
1*.1*3
7.69
1.86
28.7!*
112.25
6.19
29.30
8.72
1.05
55-1*1
139-33
3-93
3l+. 90

-------
                      A-20

Table 5.  Ohio River Fish Category Distribution
     at Selected Locations (cont'd.)•

              KILOGRAMS/HECTARE
Sample
Location
GALLIPOLIS
Sport A
Sport B
Comm.
Rough
Misc .
MELDAHL
For A
For B
Sport A
Sport B
Comm.
Rough
Misc .
MARKLAND
For A
For B
Sport A
Sport B
Comm.
Rough
Misc.
CAMELTON
For A
For B
Sport A
Sport B
1968
3.62
1.65
78.82
184.38
1.45
0.16
63.69
6.09
8.13
118 AT
221.03
3-23
0.10
315.05
2.02
0.38
123.36
339-77
13.61
0.49
9.23
28.33
1.05
1969
1.21
2.00
83.87
136.87
0.63
0.69
16. Bk
6.30
6.88
67.27
97-40
0.10
0.05
274.63
1.79
203.28
342.55
5.60
0.12
1192.20
22.17
1.96
1970
8.83
5-71
124.36
153.06
1.1*5
0.48
31.03
7.72
6.60
84.92
98.0)1
26.02
7^.22
2.62
0.74
107 • 37
467.41
7-03
47.29
30.22
0.15
1975
5.16
9-49
83.02
17.59
15-17
239.69
23.65
22.78
113.56
252.34
28.67
210.55
27.46
45-33
92.35
2.77
2.86
90.42
30.63
26.67
1976
0.80
8.31
57-45
3.21
217.10
5-27
1.10
96.43
64.44
21.79
0.23
92.74
7.16
0.08
61.73
222.50
16.02
2.83
266.37
9-32
4.6i

-------
                                   A-21

            Table 5.  Ohio River Fish Category Distribution
                 at Selected Locations (cont'd.).

                          KILOGRAMS/HECTARE
Sample
  Location

CANNELTOH

Comm.
Rough
Misc.

L & D #50.52
SMITHLAND
1968
1969
1970
50.60
78.88
10.62
52.51
11+6.93
3-90
11+7.10
170.81
13.1+0
21+9.22
21+9.88
10.31+
181.63
171+.06
9-70
For A
For B
Sport A
Sport B
Comm.
Rough
Misc.
0.02
11*03.09
33.35
2.67
190.17
105-35
0.32
0.06
1+7.25
26.71
0.11+
27.69
18.81+
10.39
55 A8
27.22
1.82
132.69
55-77
5.U8
1.50
1+38.58
9-88
3.91
297 • 08
113-17
21.23
161+5.03
10.67
0.63
50.70
32.1+1+
53.65

-------
  A-22



















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-------
                  A-23

Table 7.  Fish Biomass Estimates (Kg/ha)
      for 100 Mile River Segments
     Number of              Number of              Number of
River Section
0
100
200
300
1+00
500
600
TOO
800

900
- 100
- 200
- 300
- 1+00
- 500
- 600
- TOO
- 800
- 900

- 981
195T-59
1+2.8
ll+O.T
103.3
90.9
9U.T
T8.6
258.9
535-3
lte.7

136.2
Samples
11
9
6
15
13
T
31+
11
9

9
1968-TO
1+01+.8
156.3
290.6
-
290.1+
T59-5
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)
Samples
6
3
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0
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3
0
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3

1975-76
251.3
255-7
160.5
-
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638.1+
-
652.9
)
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)
Samples
1+
2
2
0
2
2
0
2

2


-------
                                   A-24
Table 8. Comparison of Most Abundant Ohio River Fishes 1957-59 vs.  1968-76.




Species Abundance  (based on number)
        Rank




         1




         2




         3




         4




         5




         6




         7




         8




         9




        10
    1957-59




 emerald shiner




 gizzard shad




 freshwater drum




 mimic shiner




 channel catfish




 silver chub




 black bullhead




 threadfin shad




 blue catfish




 sand shiner
Species Abundance (based on weight)
        Rank




         1




         2




         3




         4




         5




         6




         7




         8




         9




        10
1957-59




 gizzard shad



 carp




 channel catfish




 freshwater drum




 emerald shiner




 skipjack




 flathead catfish




 blue catfish




 black bullhead




 river carpsucker
   1968-76




emerald shiner




gizzard shad




channel catfish




freshwater drum




brown bullhead




mimic shiner




bluntnose minnow




sand shiner




threadfin shad




silver chub








   1968-76




gizzard shad




carp




channel catfish




freshwater drum




brown bullhead




bigmouth buffalo




emerald shiner




smallmouth buffalo



river carpsucker




black buffalo
  *most likely brown bullhead

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