CONSOLIDATED WATER QUALITY SURVEY

                               OF  THE

                           POTOMAC ESTUARY
                                1970

                             DATA REPORT

                              Number 25
    Annapolis Field Office
          Region III
Environmental Protection Agency

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                 Annapolis Field Office
                       Region III
             Environmental Protection Agency
                    1970 Data Report


                        Number 23>
            CONSOLIDATED WATER QUALITY SURVEY

                         OF THE

                     POTOMAC ESTUARY
                         Staff:

  James W. Marks, Chief, Analytical Laboratory Section
Orterio Villa, Jr., Chief, Development Laboratory Section
             Anna R. Favorite, Statistician
            Evelyn P. McPherson,  Technician

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                      TABLE OF CONTENTS
                                                    Page




  I  INTRODUCTION	   1




 II  STATION LOCATIONS	   8




III  SURVEY RESULTS	  10




 IV  MAP	appendix  i

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                       I INTRODUCTION




A.   Purpose and Scope




     In the latter part of March, 1970, the Annapolis Field




Office, Region III, Environmental Protection Agency initiated




the Potomac Estuary Consolidated program to replace the Po-




tomac Estuary Transport Survey  (1969-1970, Data Report 20)




and the Potomac Estuary DO Budget Surveys  (1970, Data Report




26).  The purpose of this monthly sampling was to continue




monitoring water quality trends in the Potomac River basin.




B.   General Remarks




     Most stations included in  this report were sampled nine




times during the year  (March through December), starting from




Key Bridge and terminating at Point Lookout.  All surface and




bottom samples were taken at mid-channel.




C.   Sampling Procedures




     Samples were obtained using a small submersible pump at-




tached to a wire cable calibrated in feet.  The pump was low-




ered to the desired depth and allowed to run for two minutes




before receiving in plastic containers (surface samples were




taken one foot from surface, depth samples five feet off bot-



tom).  Analyses for nutrients, carbon and chlorophyll a were




made from these containers.  Dissolved oxygen (DO) samples




were obtained directly from the pump outlet extending to the




bottom of a conventional 300 ml DO bottle.  The sample was

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allowed to overflow the bottle several times and fixed im-




mediately.



     A sterile loO ml prescription bottle was filled for




bacteriological analyses.




     All samples were immedietely stored on ice and analyses




started upon return to the laboratory, generally within two




hours of sampling.




D.   Measured Parameters and Analytical Methods




     1.   Water temperature was read from a Beckman Salinometer.




     2.   Conductivity was read from a calibrated Beckman Sal-




inometer.




     3.   Salinity was determined with a calibrated Beckman




Salinometer.




     k.   Light extinction, in inches, was read with a 12"




(30 cm) white secchi disk.




     5>.   Total Phosphorus




          Reference:  Menzel, D.W. and Corwin, N., 1965.  The



Measurement of Total Phosphorus in Seawater Based on the




Liberation of Organically Bound Fractions by Persulfate Ox-




idation.  Limonology and Oceanography, 10:  280-282.




          Murphy, J. and Riley, J.P., 1962.  A Modified Single




Solution Method for the Determination of Phosphate in Natural




Waters.  Analytica Chimica Acta, 2?:  31-36.




          Total Phosphorus was determined after persulfate

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oxidation  of  the  sample  in  an autoclave  at 15 psi for 30



minutes.   The resultant  ortho-phosphate  was then determined



colorimetrically  as  the  molybdenum-blue  complex with the op-



tical density measured at 882 HIM-.



     6.    Inorganic  Phosphorus



           Reference:   Murphy, J. and Riley,  J.P., 1962.   A



Modified Single Solution Method for  the  Determination of Phos-



phate in Natural  Waters.  Analytica  Chimica Acta, 2?:  31-36.



           Inorganic  Phosphorus was determined by automation



of  the above  procedure using  the Technicon "Auto Analyzer."



The molybdenum-blue  complex formed was determined colorimet-



rically with  the  optical density measured at 885 m|J-.



     7.    Total Kjeldahl Nitrogen



           Eeference:   Standard Methods for the Examination of



Water and  Wastewater,  12 ed.,  1965.



           Total Kjeldahl Nitrogen includes ammonia and organ-



ic  nitrogen and was determined by the standard micro-kjeldahl



procedure.  The sample was  digested  in the presence of strong



acid to convert the organic nitrogen to  ammonia.  The  ammonia



was then distilled, collected in boric acid  solution,  nessler-



ized and determined colcrimetrically.



     8.    Nitrate + Nitrite




           Reference:   A  Practical Handbook of Sea Water  Analysis,



J.D.H. Strickland and  T.R. Parsons,  Bulletin 167,  Fisheries

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Research Board of Canada, Ottawa, Canada, 1968.



          Nitrate plus nitrite nitrogen was determined by



automation of the above procedure using the Technicon "Auto



Analyzer."  This procedure utilizes cadmium reduction of



nitrate to nitrite and subsequent diazotization with sulfan-



ilamide and N-(l-naphtbyl)-ethylenediamine dihydrochloride with



the optical density measured at 5L\0 roH.  The results were re-



ported as nitrogen.



     9.   Ammonia



          Reference:  Southeast Water Laboratory, FWQA,



Methodology for the colorimetric determination of ammonia



by the phenol-hypochlorite reaction.



          FWPCA Methods for Chemical Analysis of Water and



Wastes, November 1969.



          Ammonia nitrogen was determined by automation of



the phenol-hypochlorite procedure as described in the South-



east Water Laboratory Methodology and later adopted as the



official FWPCA procedure.  The intensity of the indophenol



blue color, formed by the reaction of ammonia with alkaline



phenol-hypochlorite, was increased using sodium nitroprusside



as an intensifying agent.  The optical density was measured



at 630 mM- and calculated as NH^-N.



     10.  Dissolved Oxygen




          Reference:  FWPCA Methods for Chemical Analysis of

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Water and Wastes, November 1969.



          Dissolved Oxygen was determined by the azide modi-




fication of the basic Winkler method with the titration done




potentiometrically with a Fisher automatic  "titralyzer."




     11.  Biochemical Oxygen Demand




          Reference:  Standard Methods for  the Examination of




Water and Wastewater, 12 ed., 1965.



          The Biochemical Oxygen Demand was determined by the




azide modification of the basic Winkler method with the titra-




tion done potentiometrically with a Fisher  automatic  "titraly-




zer."  The samples as received were diluted if necessary and




transferred to standard 300 ml BOD bottles  in triplicate.  One




initial DO and two final DO determinations  were used  throughout.




Incubation was started immediately at 20*0  and continued for




five days after which they were titrated.




     12.  Total Organic Carbon




          Reference:  FWPCA Methods for Chemical Analysis of




Water and Wastes^ November 1969-




          Total Organic Carbon was determined with a  Dow-Beck-




man Carbonaceous Analyser after the sample  had been purged with



nitrogen gas for five minutes.



     13.  Total Carbon




          Reference:  Beckman Instruments,  Bulletin 1|.05>9.




          Total Carbon was determined with  a Dow-Beckman Carbon-

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aceous Analyser using the sample as received.




     Ik-  Chlorophyll a




          Reference:  A Practical Handbook of Sea Water Anal-




ysis, J.D.H. Strickland and T.R. Parsons, Bulletin 167, Fish-




eries Research Board of Canada, Ottawa, Canada.  1968.




          Chlorophyll a was determined by extraction  of milli-




pore filtered samples in 90% acetone and read spectrophoto-




metrieally.




     15.  Coliform




          Reference:  Standard Methods for the Examination of




Water and Wastewater, 12 ed., 1965.




          Coliform population was determined using the 5>  tube-




3 dilution Multiple-Tube Fermentation Technique.  Lauryl




suj.fate tryptose broth was used for the presumptive test  with




incubatior. at 35°±0.59C.  Brilliant green lactose bile broth



was ased for the confirmatory test with incubation at 35<>+0.5°C.




The results were reported as the Most Probable Number (MPN).



     ifc.  Fecal Coliform




          Reference:  Standard Methods for the Examination of




Water and Wastewater, 12 ed., 1965.




          Water Pollution Control Research Series, WP-20-3.




          Fecal Coliform population was determined using  the




5 tube-3 dilation Multiple-Tube Fermentation Technique.   Lauryl




sulfate tryptose broth was used for the presumptive test  with

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incubation at 3!?e±0.f>*C.   EC medium was used for the confirm-



atory test with air  incubation at ii5>.50±0.5"C.  The results



were reported as  the Most Probable Number (MPN).

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                   II STATION LOCATIONS






Station               Location
 1                     Key Bridge



 1A                    One-half mile above Three Sisters  Island



 2                     ll|.th Street Bridge



 3                     Haines Point, Buoy 2



 3B                    North Bollingfield, Can 9 Main Channel



 k                     Bellevue, Nun 8




 liB                    Blue Plains Effluent



 5                     Woodrow Wilson Bridge



 5A                    Rosier Bluff



 6                     Broad Creek,  Nun 86



 7                     Piscataway Creek, Buoy 77



 8                     Dogue Creek,  Fl 67



 9                     Hallowing Point, Can 61



10                     Indian Head,  Nun 5h



11                     Possum Point, Nun kk



12                     Sandy Point,  Nun 1|0



13                     Smith Point,  Nun 32



^k                     Maryland Point, Can 19



15                     Nanjemoy Creek, Fl 13



ISA                    Mathias Point,  Fl 5




16                     Route 301 Bridge, Buoy CN

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Station               Location
17                    Upper Machodoc, FI 30 or off Piccowaven Ck.




18                    Kettle Bottom Shoals, Fl 2£ or M




18A                   Cobb Island, Can 15 or RB N




19-3                  Wicomico Creek, Nun 2₯




19-2                  Wicomico Creek, Can 7W




19-1                  Wicomico Creek, Beacon 16W




20                    Kingcopisco, BWN £2B




21                    Bagged Point, BWN 5lB



22                    Piney Point, BWN £OB




2?                    Point Lookout, Fl 1; Bell

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   AT HI AS POINT. Fl_ S - I5A.

       ROUTE  301 aniDOE BUOV C N -* ' 6

                WiCOMtCO CREiK. 8EACOM ISW- IB
  CONSOLIDATED WATER QUALITY SURVEY
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         POTOMAC ESTUARY
              1070
            SCALE M MLCS
POTOMAC    ESTUARY

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