EVALUATION  OF  WATER  QUALITY   TRENDS


       SHENANDOAH   RIVER,  VIRGINIA




       FRONT  ROYAL  TO  BERRYVILLE



                                 JOHN  W BAUMEISTER
            TECH NIC A L   RE POP T  2
           U.S. DEPT.  OF  THE  INTERIOR
         FEDERAL WATER  POLLUTION CONTROL ADMIN
                MIDDLE  ATLANTIC REGION
                  918  EMMET STREET
         C HARLOT TE SV I L LE                VIRGINIA
                   DECEMBER  1968

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                TABLE   OF   CONTENTS






                                                               Page




Acknowledgements	.	..«,..,.,  ii






Introduction	   1





Summary	..........................I..............   2






Water Quality	   k



     Eutrophication and Phytoplankton	   5



     Bottom Fauna	.   8






Conclusions	  11






Bibliography	  12






Appendix - Tables	,	  15

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                         ACKNOWLEDGEMENTS






        The author is deeply indebted to Mr. Eugene >/. 3urber,




Virginia Commission of Game and Inland Fisheries, who graciously




supplied long-termed bottom fauna data which were invaluable in




showing eutrophication trends in the Shenandoah River,  Bottom




fauna information provided by Mr, John E.. Tackett, Virginia State




Water Control Board was also very useful in this evaluation.




        Grateful acknowledgement is expressed to Mr. Lowell E.




Keup, Technical Advisory and Investigations Branch of the Federal




Water Pollution Control Administration, Cincinnati, for his




helpful suggestions in editing this Report*

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I.      INTRODUCTION





        The 1T« 3. Army Corps of Engineers Western Virginia Area Office



near .Jorryville, Virginia, obtains its water supply from the Shenandoah



River,  In recent years, Corps officials have expressed concern with



increased pollution and the presence of certain planktonic organisms



found in untreated river water and. problems associated with removing



them in water purification systems.  In anticipation of a potential



need to alter water treatment facilities, (k>rps of Engineers officials,



by letter dated May 9, 1968, requested the Middle Atlantic Region,



Federal Water Pollution Control Administration, to determine pollution



trends in the i^-iver.



        This Report contains the findings of our evaluation of water



quality trends in the Shenandoah River.  The nhysical, chemical and



biological information investigated was obtained fro.-n several sources.



These include data collected at the Federal Viater Pollution Control



Administration T
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IT,     SUMMAHT





        The lower /Jhenandoah River has had a long history of pollution



problems, dating back to i9kO when toxic industrial wastes discharged,



in the stream all but decimated aquatic life downstream from Front



Royal„  Subsequent abatement of this pollution resulted in the re-



covery of the River| however, increased discharges of organic wastes



and, runoff from agricultural lands have caused significant nutrient



enrichment.



        Physical and, chemical data collected, at  the Berryville



Surveillance Station were evaluated to determine pollution and



eutrophication trends in the main stem downstream fr-am fr-oat Hoyal.



It was found, that the chemical quality was within the raw water



criteria established for public water supplies:  however, total phos-



phorus and nitrogen content of river water was sufficiently high



to i'ndicate a fertile stream capable of prodv:j;;',g abundant aquatic



organisms,



        Eutroph,icatic.r trends are also borne rwt by 'r;^logical data



collected near Berryvill^.  Thirteen years of t-cttosn samples collected



over a 30-year period in this reach show a gradual ircrease in the



productivity of aquatic invertebrates.  For the  most part, these



samples were comprised of clean water and faev.j.ta',;,v© forms which



indicate the presence of relatively clean water0



        One of the most significant indicators cf organic enrichment



in the lower Shenandoah River are the changes an phyt^'lankton popu-



lations from .1962 through, I960.  Phytoplarnktcn rambers and population



composition dvring this period indicate a change froro a diatom dominated

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population to one dominated, by green algae.  Although several species




of nuisance algae were found in Rerryville samples, at no time were



they present in quantities sufficient to cause water treatment



problems,

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III.    WAT3R QUALITT




        The Shenandoah River originates  in  the  roantalns  of Northern


Virginia, the Hcrth 7ork in the Allegheny Mountains  and the South


Fork in the Blue Ridge (Figure I),   Each fork fluws  -j.r.prr»xirr,ately


15>0 miles through fertile limestone  valleys to  front Royal, Virginia,,


From Front Royal, tre w.aiR stein  flows  north ab<^,;t 60 miles before


entering the Fctomac at Haroers  ferry, West "Virginia^  The Corps of


Engineers' raw water Intake is located on the main ster approximately


30 miles downstream from Front Royal near the Town of Berryville,


Virginia.


        Recent investigations of the Shenarctoah River system


(Tackettj 1962, 63; Surber, 196/4,  65,  66) indicated  that  the North


and South Forks are relatively free  from the serious effects of


water quality degradation,.  At Front Royal^ the South Fork receives


treated organic and rhe^jca] wastes  from a  synthetic fibers company,


untreated organic wastes fron a  fruit  cacking company, and sewage


which has under gone primar;/ treatment  from  the  City  r.f Front,, Royal,


        Tn order tt> determine the  effects of this pollution on


water quality in the lower Shenandoah  River, an anajyais  was made of


water quality data col levied, at  the  FWPCA w'ater PC] lotion Surveillance


Station located at the "->rps f>f  Engineers* water supply intake near

           ^
Berryville/1  These data were compared to the raw wa^er standards for


public water supplies and criteria required to  naints,ar> a favorable


environraent for fish and -:-iher aquatic life developed, by  the National


Technical Advisory Committee en  Water  Quality  Criteria,
      Unpublished  'imOii'E,T  data,  FWPCA Surveillance Station No, 51008?.

      Shenandcah River at Berryville,  Virginia0

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                                                                   5




        Based on our analysis of composite samples collected at



Berryville between 1962 and 196?, the chemical constituents of



Shenandoah River water were within the raw water criteria estab-



lished for public water supplies.  However, wastes discharged into



the River at Front Royal have had. an adverse affect on water quality



and. aquatic life immediately downstream from the source,  These



wastes and runoff frorc agricultural lands are also important sources



of nutrients which influence phytoplankton growth in the lower river,





Eutrophication arid Phytoplankton



        Eutrophication is a term that is commonly used today to mean



the nutrient enrichment of waters by man-created or natural means.



Urban and industrial growth have combined to increase the fertili-



zation of lakes and streams through the discharge of nutrients in



various forms.  Nutrient enrichment has resulted in aquatic weed and



algal nuisances in areas where before this did not exist.  One of



the major prc.ble.tis associated with excessive: growths of aquatic



vegetation is the interference which these t>rgants'ns have in the



treatment of a potable water supply.  Many forns of algae have been



known to impart undesirable tastes and, odors to drinking water, while



others have clogged filters In water treat-vent -l-nts,..



        Within the past two decades, there ha? been a jignificant



increase in the frequency of plankton blocks in the Shenandoah River.



Surber (19-6U) noted the difficulty in counting smallmouth bass nests



downstream from Front Royal because of a phytcpJankton bloom which



had. occurred and indicated that "blooms" were not known to have



occurred, prior to

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         In 1965 >  ^'Jrher rer-.-^r'ied  a four-fold  Increase  in turbidity



on the  South K'oiv in *be  strevn re'tch from Goori:-: lj!;|.]  t^ vront; Royal,



which he attributed, t* thytor.-lankt&n.  He  concluded that, -iignlf leant



eutrophication had  occurred sfier L9  that date



the South Fork was  3s clear as  the North Fork v.~. at the rresent time,



This eutrcphicat^n trend was 'junslantia^ed fu^Uc3:* hy  *"hartf;es in



vegetatione  rn  the r«risd between 19ltO and 19SX coarse v-aedr-;



choked  the Hiver, ivst soir-eti-'*?  later chjri'jr.lfi'ik*. .n '.ie?^:f ; >ed  in



such abundance  'hat. ",he quantity  of light  available to  rioted



aquatic vegetat.-.nr-  was greatly  reduced,  As a rest!?,,  the rr-arse



vegetation decreased in abundance0



         Prosent  *-• now! edge indicates that njini^en  (K;  -^rH rhfGphorus



(P) are the ferti1izing elements  which contribute mc-st  tu -increased



growths of a/iua',v,  '•:&£e:;i',•'•*.>" and alga] bjco^~o  Fir.n  *•   -''ircri'



trations resn.lt  m  rhvwr-] nnkt',^ *x>t?iulati'.'< di.>>.' snsted  by freen



algae and z rus?,^. ow-nKjefitr^' "• •*!  of P e»:^Hr,..a-;;   * ht- ;rrv^fr. -;f "cl'r.e-



green? 0  l>,e hor-T*  •!" fl*i° '-.*:< oir\ 1 Tecnr i:\-i.! .-.-3v-.i. -y  ,: .,-rjit -.ee



on ".'ate i* ^oliiv Cf'orr.--i •'"ii''6i1]'  i-tilr-* "•"•   ••• ' TJ-J •",". f f^."j-«-;\ ^^ ecio?



of algae ha"?e -j iff pn-.^-,. :-*i^ji ^C'r"j3 re').;irfe' t^*-•> witt  •?  ^^'U/e of



avaiJable r.hc•>:-,:her  ;.-- ...^••;'31 ;,v  fallinfr iretwe<-j'- OA!  ,=srd  ''-..03- ^g/lc



At these levels, wV en otht;'- coridit lo>r>j- ar-e lvivjr-.it:ie   ulc.-oris. .i>ay



be expectecic   'r>e forrcn ttee 3} so repc-rted  th^t  w-fh ccncer'trations



greater t,har* Orir HJP/I in flawing waters,  proWe^' with excessive



algal gi'cw*hs ^ay be pfnrrv,;nfer^Ti.  Tn^ r'rtrof'en-fliosphorus tatlo



is also of iT.pC'rtance in  natural  waters as an indicator of * he



type of algae which cry) be expected to grow,   k 1,'J 1  ratio has been

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                                                                  7




established as a guideline for indicating normal conditions *



        Three years of nitrogen and phosphorus data were available



for analysis from the FWPCA Surveillance Station,  Nutrient concen-



trations in the lower Shenandoah River during the period 1965-196?



were high with total phosphorus ranging from 0*01 arid 0.33 mg/1.



During this period, the 0,10 mg/1 guideline was exceeded five times



(22% of samples taken), indicating fertile conditions favoring



dense plankton blooms„  Levels of total nitrogen averaged, 1 ,k mg/1



and ranged frorn less than 0»1 mg/1 to h,3 rag A'  Inorganic nitrogen



compounds were also found at high concentrations adding to the



overall fertility of the stream.  In addition, fertile conditions



were exemplified by the nitrogen-phosphorus ratio which exceeded



the lOsl guideline in 83% of samples taken with a high of 108-1



recorded on May 3* 19670



        Perhaps one of the most striking indications of eutrophi-



cation in the lower Shenandoah River is the pr^iounred cbange which



has occurred in the composition of the plankton pcpvJa*IOP,  Accord-



ing to the National Technical Advisory Committee on Water Quality



Criteria, conditions indicative of organic enrichment irclud.e a



change from a diatom-dominated, plankton population to one domi-



nated by blue-green and/or green algae5 associated with increases



in amounts and changes in relative abundance of nutrients,  Plankton



samples collected at Berryville during the period 1962-1965 show



a significant increase in green algae with a corresponding decline



in diatoms (Figure 2),  AlthoxJigh the water was not analyzed for



nitrogen and phosphorus content during this period, this shift in

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                                                                  8
plankton population supports the conclusion that eutrophication has
taken place in the lover Shanandoah River.
        Algae samples were also evaluated to determine the presence
of species known to cause problems in water treatment and if con-
centrations were sufficient to warrant concern.  Several algal forms
were found that have been known to clog filters in water treatment
plants, including the diatoms synedra, navicula, nitzschia and
others.  However, at no time were the numbers of these organisms
sufficient to result in serious filter clogging problems.

Bottom Fauna
        In considering biological conditions within a flowing stream,
the quantity and species composition of the bottom organism community
can be indicative of water quality.  Because the life histories of
many bottom aquatic invertebrates are one year or longer and because
these organisms are relatively immobile, they are invaluable indi-
cators of long-term water quality conditions.  For example, pollution
of organic wastes may fertilize a stream to the point at which
certain kinds of bottom animals become very abundant.  Conversely,
the continuous exposure of bottom animals to toxic chemicals can
cause complete mortality.  It is possible to classify bottom animals
as to their sensitivity to pollution, tolerance of it, or somewhere
in between (facultative).  In graphs found in this report, the
bottom animals have been designated as pollution tolerant, facul-
tative or able to adapt to mildly polluted conditions, and sensitive
forms which are intolerant of pollution.

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        The number of bottom organisms collected in the Shenandoah
River below Front Royal has varied considerably during the period
1936-1967.  In the early 19UO»s, highly toxic industrial wastes
containing high concentrations of zinc were discharged into the
River at Front Royal and all but decimated bottom fauna and fish
populations downstream from the pollution source (Figure 3).
Implementation of improved waste treatment facilities in the late
l?J*0's produced improved water quality and subsequent return of
large numbers of invertebrate bottom animals.  Comparing the number
of organisms per square foot of bottom collected in 19ii8 to numbers
in 1965, shows a significant increase in bottom fauna downstream
from the main source of pollution (Figure It).  Although part of
this increase can be related to removal of toxic wastes from the
stream, the primary reason for the substantial upsurge in numbers
can be attributed to organic enrichment or eutrophication, which
provides increased quantities of food for these animals.
        Recent bottom fauna studies of the Shenandoah River
system indicate that the North Fork of the Shenandoah is rela-
tively free from the serious effects of water quality degradation.
The South Fork is also characterized by good water quality through-
out most of its length except at Front Royal where it receives
industrial and domestic wastes.
        While conducting field investigations of the Shenandoah
River, Tackett (1963) found that the South Fork immediately down-
stream from Front Royal was badly polluted with organic wastes and
contained dense growths of SphaerotilUB. a filamentous bacteria

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                                                                 10
characteristic of exceptionally poor water quality.  Consequently,
this sector of the River was practically devoid of bottom-dwelling
invertebrates.
        Pollution entering the South Fork at Front Royal has also
influenced water quality and the aquatic biota in the Main Stem
downstream from the confluence.  In recent years, this reach of the
River has experienced several fish kills, some of which resulted
from dissolved oxygen deficiencies during low flow periods.  However,
the cause of some of the larger fish kills has remained unsolved,
which has led to continuing biological investigations in the River
downstream from Front Royal.  Adverse water quality conditions are
also demonstrated by the bottom samples taken at Morgan Ford, ten
miles downstream from Front Royal.  Review of data from this station
indicates a bottom community comprised of more pollution-tolerant
forms and fewer sensitive species than were collected at stations
located upstream from the pollution source (Figure 5).  However,
the increase in clean water bottom fauna collected at Berryville
shows that the stream has almost totally recovered from the very
poor water quality conditions found upstream.  Surber, (1966) in
analyzing the results of bottom sampling concluded (l) the North
and South Forks remain rich in fauna and apparently unaffected by
pollution, (2) pollution adversely affects the bottom fauna of the
Main Stem of the Shenandoah River for at least half its length in
Virginia.

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I?.     CONCLUSIONS

        Biological and chemical water quality data can be summarized
as follows:
        (1)  Constituents of river water at Berryville were within
the raw water criteria established for public water supplies.
        (2)  Treated organic and inorganic wastes discharged into
the South Fork at Front Royal have an adverse affect on bottom
fauna in immediate downstream reaches.
        (3)  As the River recovers from this pollution, the en-
vironmental response is an increase in numbers of bottom fauna
near Berryville.
        (Ij)  Phosphorus and nitrogen concentration in water samples
taken at Berryville are sufficient to cause phytopiankton blooms.
        (5)  Changes in phytoplankton populations from one pre-
dominantly diatoms to a population of predominantly green algae are
indicative of organic enrichment in the lower Shenandoah River.
        (6)  Phytoplankton nunbers have not increased to the point
where they could cause serious problems in the treatment of potable
water.

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A SAMPLIKO STATIONS

SHENANOOAH  RIVER  SYSTEM
  SHOWING BOTTOM FAUNA
     SAMPLING STATIONS
                       FIGURE 1

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                                                                  12


BIBLIOGRAPHY
1.  Anon,
       1968.   Water Quality Criteria.  Report of the National
               Technical Advisory Committee t© the Secretary of
               the Interior,  Federal Water Pollution Control
               Administration3, U. S. Department of the Interior,
               Washington, D0 C., x + 23k ppc

2.  Anon.
       1963.   National Water Quality Network.  Annual Compilation
               of Datas October 15 1961 - September 30, 19620
               Public Health Service Publication Mo» 663 (1962 ed.)
               xi * 909 pp°

3 o  Anon.
       196U.   National Water Quality Network,  Annual Compilation
               of Data,, October 1, 1962 - September 303 1963*
               Public Health Service Publication No, 663., Vol. 2
               (1963 ed.)  viii - 119 pp»

U.  Davis, W. S.
       N.D.    ohenandoah River Report,  U0 S. Fish and Wildlife
               Service, Kearneysville, West Virginiac  (Unpublished,
               report^ 5 pp.-)

5.  Davis, W. 3.
       N.D.    Shenandoah Fiver Report,  Uc S,, Fish and Wildlife
               Servicea Kearneysville, West Virginiac  (Unpublished
               report,, 5 pp«)

6.  Davis, ¥. 3,
       N.D,    Shenandoah River Report.  IL 3,, Fish and Wildlife
               Service, Kearneysville,, West Virginia,  (unpublished
               report, 5 PF»)

7.  Davis, W. 3«
       N.D.    ohenandoah River Report.  U0 S* Fish and Wildlife
               Services Kearneysville,, West Virginia.  (iJnpyblished
               report, 5 PP=)

8.  Davis, W, S,
       N.D.    Shenandoah River Report.  Uc S. Fish and Wildlife
               Service,, Kearneysville, West ?irginia0  (Unpublished
               report, 8 pp.)

9.  Henderson, C.
       19U9.   Value of the Bottom Sampler in Demonstrating the
               Effect of Pollution on Fish Food Organisms and Fish
               in the Shenandoah River,  The Progressive Fish
               Culturist, 11  (I9li9)s 217=230 pp.

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                                                                  13
10.  Henderson, C,
        N.D.    Shenandoah River Investigations<.  U. Se Fish and
                Wildlife Service, Kearneysville, West Virginia,
                (Unpublished report, 11 pp»)

11.  Henderson, C,
        N.D.    Shenandoah River Investigations.  U. S, Fish and
                Wildlife Service, Kearneysville, West Virginia,
                (Unpublished report, 7 pp.)

12.  Keup, L. E., W. M. Ingrain and K0 M. Mackenthun
        1966<,   The Role of Bottom-Dwelling Macro fauna in Water
                Pollution Investigations.  Department of Health,
                Education and Welfare, Cincinnati, Ohio,  Public
                Health Service Publication No. 999-WP-38, v + 23
                PP»

13.  Mackenthun, K. M«, and W. M0 Ingram
        1967.   Biological Associated Problems in Freshwater
                Environments, Their Identification, Investigation
                and Controlc  U. So Department of the Interior,
                Federal Water Pollution Control Administration,
                Washington, Do C0  x + 287 pp.

lli.  Mackenthun, K. Me
        1965.   Nitrogen and Phosphorus in Water, An Annotated
                Selected Bibliography of Their Biological Effects.
                Robert A. Taft Sanitary Engineering Center,
                Cincinnati, Ohio»  U. S. Public Health Service
                Publication No, 1305, xxviii -*- 111 pp.

15.  Palmer, C. M.
        1962.   Algae in Water Supplies,,  U. S. Public Health
                Service Publication No. 6$7» Washington, D0C0,
                vi -*• 88 p00

16.  Palmer, C. M.
        1967.   Biological Aspects of Water Supply and Treatment
                in Virginia With Particular Reference to Algae.
                Virginia Journal of Science 18 (l)i 6-12,

17.  Surber, E. W.
        1967.   Smallmouth Bass Stream Investigations.  Job No. 2,
                Shenandjoah River Study, 196lu  Commission of Game
                and. Inland Fisheries, Richmond, Virginia,  30 pp.
                (mimeograph).

-------
                                                                   11*
18.  Surber, E. ¥.
        1966,   Smallmouth Bass Stream Investigations *  Job No. 2,
                Shenandoah River Study, 1965.  Commission of Game
                and Inland Fisheries, Richmond, Virginia.  80 pp.
                (mimeograph).

19.  Surber, E. W.
        1967.   Smallmouth Bass Stream Investigations.  Job No« 20
                Shenandoah River Study, 1966,  Commission of Game
                and Inland Fisheries, Richmond.,, Virginia*  72 pp.
                (mimeograph).

20.  Tackett, J. H.
        N. D,   Shenandoah River Basin, Biological Assessment of
                Water Quality, October 1963.  Virginia State Water
                Control Board, Richmond, Virginiat  76 ppc
                (mimeograph)„

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APPENDIX
    TABLES

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  Date

10/2U/62
1/23/63
it/3/63
7/2i;/63
10/16/63
1/22/6U
U/22/6U
7/29/6U
10/1U/6U
1/20/65
U/21/65
7/28/65
10A2/66
1/18/67
5/2t*/67
7/12/67
l.O/U/67
TABLE 1.


    D,0,
    rog/1

     8,6
    13o3
     9.2

     8.U

     9.2
                      SELECTED WATER CHEMISTRY DATA.
                      SHENANDOAH RIVER NEAR BERRTVILLE, VIRGINIA
8.3
8.U
8.3
8,h
8.2
808
8,3
B.k
8.2

8°.3
               801
                                    Alkalinity
                                    ______ mg/1 _

                                       156
                                        92
                                       120
                                        7k
                                        76
                                       120
                                        88
                                        76
                                       Ilk
                                        97
                                        8l
                                        80
                                        97
                                       131
   Hardness
     192
     128
     12U
     160
     196
      90
      90
     172
     200
     1214
     105
     180
     10?
      92
      86
     126
     155
Sulfates
  mg/1

   9h
   31
   26
   90
  150
   3U
   38
  138
  150
   57
   39
   70
   iil
   k3
   3k
   86
   50
I/
     STORST data0  FWPCA Surveillance Station No<
     River at Berryville, Virginia,
510087.  Shenandoah

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       TABLE 2.   NITROGEN AND PHOSPHORUS CONCENTRATIONS IN THE
                  SHENANDOAH RIVER NEAR BERRXVILLES VIRGINIA,
                  1965-1967. I/

Date
1/6/65
2/3/65
3/3/65
a/7/65
5/5/65
7/7/65
9/8/65
10/6/65
11/3/65
1/5/66
3/9/66
a/6/66
5A/66
6/8/66
7/6/66
8/3/66
9/7/66
10/5/66
11/2/66
12/7/66
1/7/67
2/8/67
5/3/67
7/5/67
9/6/67
Total
Phosphorus
0.05
0,02
0,38
0,05
0,02
Oo05
0006
0,03
0.01
0,02
0«06
~ «.
0.15
o»o5
0,07
O.lli
Q008
Ool2
O.Oii
_ =
0,03
« ^
OoOii
Oo2
O.Oli
(All values in milligrams per liter)

                     Ammonia N

                     Organic N


                        0.5

                        Oo3


                        2.8

                        o!e
                        0,2

                        Co?
                        1.3
                        1.0

                        1.3


                        0*2
                                          0.3
                                          3.U
                                                           Nitrate N
                                                           Nitrate N
                                                              0.7
                                                              0,6
                                                              0.7
                                                              006
                                                              Ocii
                                                              0.1
                                                              l.U

                                                              0.5
loll
0.8
0,8
1.0
1.0
                                                              O
I/
     STORET data.  FWPCA Surveillance Station No. 5l0087<
     Shenandoah River at Berryville, Virginia„

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          TABLE 3.  PHITOPLANKTON POPULATION OF THE SHSNANDOAH RIVER
                    NEAR BERRIVILLE,  VIRGINIA FOR SELECTED DATES.  V

                             (Number  per railliliter)
Date of Sample

   1/3/62
   U/3/62
   7/6/62
   10/3/62
   1/9/63
   U/3/63
   6/19/63
   10/9/63
   1/22/6U
   U/8/6U
   7/22/61*
   10/7/6U
   1/6/65
   U/7/65
   7/7/65
   9/8/65
Blue-Green
   I/
Green
                                             Flagellated
                                             "
0
0
6,960
230
0
Uo
1,010
U3Q
20
160
0
0
0
0
0
0
20
210
9,620
1,350
0
70
17,390
5,Uoo
180
660
33,680
3 2UO
180
580
50,220
15,560
                               20
                            1,260
                             '  Uo
                                0
                               20
                               90
                              130
                               50
                                0
                            1,230
                              Uio
                              160
                               20
                              160
                              180
                              220
Diatoms
                            2,710
                            6,650
                            8,11*0
                              860
                            1.89Q
Total
110
32,000
28,700
2,8UO
1,010
UUo
li,220
2,160
200
33,500
U5,300
U,Uoo
1,000
600
22,700
8,000
            2,900
            1,200
            2,600
           53,900
           17,700
         STORET Data,, FWPCA Surveillance Station
         River at Berryville^ Virginia,
                              No. 510087* Shenandoah

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                TABLE U°   PERCENTAGE OF VARIOUS TOLERANCE FORMS
                           OF BOTTOM ANIMALS, SHENANDOAH RIVER  196U-1966 I/, 2/ ,27
     STATION

 33 Miles Upstream
 from Front Royal
YEAR

196U
1965
1966

Average
TOLERANT

   13
   11
                                         17
FjlCULTATP/E

    37
    52
SMSTT1TO

    50
    37
                                      1,0
 Front Royal
196U
1965
1966

Average %
   13
    8
   11
    33
    38
    19
                                                         UO
    5U
    5it
    Uo
   • !-••-••••i

    51
 Morgan Ford
1961*
1965
1966

Averag
   29
   30
    31
    51
                                                          38
    10
    19
    ?6
   —in—11 n im

    28
 Berry ville
1961.
1965
1966

Average %
    2
   27
   18
  Tmm|-i_ i..

   16
                       2?
                       37
                       29
                      •wnraiKM

                       ?9
—   Surber, S, W,,  196?,   Srnal 1 mc>,;th Bass Stream Investigations-   *ob No,  2,
Shenandoah Hiver  Study, L9&k»   Concussion of Gar.e ar:u  inland Fxsneries,
Richmond, Virginia. 30 pp.
2/
-   5urbers u. W»,  1966,   Smallmouth Dass '.-tres/ ! 's1':-,.* ,.7 .-  ,^,rjr,    -.'oo '::o -  2
Shenandoah River  Study, 1965«   Commission of Game anj  :'r; ^-i-i Fisrien^b,
Richmond, Virginia* 80 ppa

-   Sxjrber, Ee W.,  19660   Smallmouth Bass Stream Investigations,   -'oh Moc  2
Shenandoah River  Studyt 1966„   Ccmmission of Game and.  Inland Fisheries,
Richmondj Virginia, 72 pp.

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     TABLE 5.  AVERAGE NUMBER OF BOTTOM ANIMALS,
               SHENANDOAH RIVER, 19U8 AND 1965

                (Number per sq» ft0)

                                         Year

        Station                19^8 i/          1965 -/

33 Miles Upstream from
 Front Royal                     7lU              952

Front Royal                      937              637

Morgan Ford.                       85              ill9

Berry ville                       270              '",6


~   Henderson, C,>, 19U9.  The Value of the Bottom Sampler in
Demonstrating the Effects of Pollution on Fish Food Organisms
and Fish in the Shenand.oah firver.  The Progressive Fish
Culturist, Volume 11, No, h» pp° ?17-230,

?/
~f  Surber, E» W., 1966,  SmaUmouth Bass Stream Investigations,
Job No, 2, Shenandoah River Study,, 1965^  Commission of Game
and. Inland Fisheries, Richmond, Virginia,..  80 pp,

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