54077502
                     Mathematical

                     Evaluation

                     of
         Kepone  Action Levels
           January 10, 1977
                f

                Prepared by

        U. S. ENVIRONMENTAL PROTECTiON AGENCY
            Office of Pesticide Programs
          Office of Special Pesticide Reviews
             Washington, O.C. 20460
r.? 5^/


               LIBRARY
               .; S- ENVIRONMENTAL PROTECTION
                   «, i.

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Mathematical  Evaluation

                of

  Kepone  Action  Levels

          January 10, 1977
     By: Todd W. Thorslund, Sc.D.
         Gerald K. O'Mara, M.S.
                    \
                     *
              Prepared by

    U.S. ENVIRONMENTAL PROTECTION AGENCY
          OfficejB^Pestjcide Programs
       Office of Special Pesticide Reviews
          Washington, D.C. 20460
            LIBRARY
              5. EuVlfiONMENTAL PROTECT10-N
                M. 1. 0^817

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                        CONTENTS

Lists of  Figures	ii

List of Tables	  iii

Bac kg round	1

Introduction	3

Dose Response  Relationship.	4

Action Levels  Obtained by  Considering
Risk to Individuals	7

Action Levels  Obtained by  Considering
Risk to Total  Population	11

Estimated Risk  to Population  in  Area
Contiguous  to  Chesapenke  Bay  Under
Current Action  Lev els	16

Effect of Kepone Resicues  in  Blue fish	17

    Append ices

    Appendix  A.  Production and  Consumption
    of Chesapeake Bay  Seafood	21

    Appendix  B.  Assumptions  Used to Compute
    Implied Risk to a  Given  Population	40

    Appendix  C.   Tumor Risk  to  U.S. Populat  on
    Assuming  Log-Probit Model and Different
    Exposure  Patterns	46

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                           FIGURES
NO.                              '                   I'aR e

 !    Functional  Relationship Between
     Kepone  in Diet  and  Incidence  of
     Hepatocellular  Carcinnm,    	   19

 2    Relationship  Between Kej one  in
     Diet  and Incidenc<  of Hepatocellular
     Carcinoma in  the  !ange of  Probable
     Human Exposure	    20

 3    Rela t ionsli ip  Between Implied
     Calculated  Risk Rate and Action
     Level for Three Dietary Intake
     Levels	    21
                           ii

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                     LI  T  OF  TABLES
No.                                                    Page

1  Possible  Levels of  Seafood Consumption
   to he  Utilized In Dotermining Action  Levels	10

2  Potential Action Lex els  for Different  Lev els
   of Risk  and  Seafood  Consumption	10

3  Implicit  Calculated  Number of Tumors  Under
   Present  Actions Levels	12

4  Resulting Action Levels  Under Different
   Risk/Benefit Assumptions	14

5  Implied  Risk to Individual in Chesapeake
   Bay Area	...16

6  Lifetime  Production  of  Tumors Due  to
   Consumption  of BluefLsh	18
                          111

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                          RACKGRO 'NO




     In  the  spring of 1976,  the Office of  Pesticide Pro-




grams, EPA  evaluated Kepone  residues for Chesapeake Ray




Seafood.  As  a result, action  levels were  proposed by EPA




for fin  fish,  shellfish  and  blue  crabs and  late •  implemented




by FDA.   Current action  levels for Kepone  'neasured in parts




per million  (ppm) are:   0.1  ppm in fin fisl;  0.3  ppm in




shellfish;  and 0.4 ppm in  bluecrabs.







     At  tie  time the action  levels were  put  into  effect,




little was  known concerning  the extent an>l  duration of




the Kepone  problem for the Chesapeake B a ^ .   EPA's position




was that  as  additional inforiation became  available on  the




subject  a reexamination  of r sks  and benefits related to




alternative  action levels  wo "Id be undertaken.







     NCI  has  recently rade available an  updated report




concerning  chronic can er  bioassay studies  of rats and  mice




fed kepon>'.   In additi >n ,  data has recently  been  collected




concerning  the amount of seafood  consumed  that  is taken  from




Chesapeake  Bay.  This  'dditional  informaticn allows a




reassessment  of the ac ion levels for kepone at this time.







     This paper is intended  to outline a genera!  methodology




for assessing  the risk of  a  given action  level  and for  deter-




mining action  levels based on  implied risk  concepts.  It




uses kepone  as a case example.  The method  developed allows

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one to either  set an action  Jevel by controlling  what amounts




to a conservative estimate  of  the total  ri  k  or  a fixed




ben >fit--risk  ratio.  This  upper bound for  risk takes into




account the  total human exposure and carcinogenic response




levels of  animals.  The method is also flexible  enough to




directly  account for benefits  of the adulterated  product for




the same  action level determination.







     Several  potential problems can be anticipated in using




the rethod:  1) The ~e is a  danger that rather  tli in interpreting




the upper  bound of risk as  a carcinogenic  index,  a literal




interpretation of risk as  an actual estimate  might be made;




2)  The absolute value of  the index  is hard  to put into




context since at present  it  has not been used in  other




situations.







     It  is also recognized  that the method  suggested has




numerous  scientific shortcomings.   Its utility only becomes




apparent  when contrasted  to the traditional method of




obtaining  an action level  which is  not appropriate for




effects presumed to have  no biological threshold.

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                            IN "RODUCTION •






      In  order to assess the carcinogenic risk  to man of




low  levels of exposure  to a <• hem !•• al ,  it is  necessary




to extrapolate data  from animal  ecperiments  conducted at




high  levels of exposure.  Virtually all competent scientists




however  recognize  that  this type  cf extrapolation is at best




highly  speculative  and  based on  many unverifiable assumptions.




Thujs^j	the estimate  of  thr resulting number of  tumors produced




i n man  by th e c heroic a 1 _ s h oulid^  not be looked  a t as a t r u e ,




a_b^sj)ljj_ti; number.  Mor_e  aj propr lately ,  it^ should be viewed	as^




a health hazardindex  thct	incorporates bot_h the degree of




care i^nogejiic ac ^iv_i_ty_ o_f_ a chemical and the  exposure lev el s




to which man is  s_uspec_t_eci to be  subjected.   Ii light of




this,  the absolute  value of the  estimate is  not in itself  of




primary  importance.   Rather, the  factor to focus on is the




change  of the index due to differences in  regulatory stra-




tegies  and to a  compariscn of  the magnitude  o' risk index  of




various  chemicals.







      To  obtain a value  for the  health hazard it is necessary




to define the functimal form  of  the mathematical relation-




ship  between consump  ion level  and  tumor incidence. In




addition, methods   that should  he uniformally  followed are




needed  to chose  the data base  to  be utilized in fitting the




selected dose response  curve and  in estimating human exposure.

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                i)o_SK Ri-spnr si-  RKLATTONJHJP




     The functional form  chosen  for predicting  effects of




ingested Kepone was the "one-hit"  model wh'ch  has  many




theoretical  and practical  advantages.  Furthermore,  since a




risk estimate  is highly dependent  upon the model  chosen,




using the  "one-hit" mo lei  in  this  case also  has the  added




advantage  of being internally consistent  since  it  was




previously  employed hy  the  Carcinogen Assessment  Group  (GAG)




to predict  the ri.ck to  humans from the continued  in  term  use




of Kepone  ant-bait,  Firther, for  reasons  which are  amplified




in Appendix  C, the "ona.-hit"  model is deemed  most  appropriate




for  this purpose.  How'ver  it is recognized  that  other  models




could be utilized.  In  fact,  the Administrator's  Interim




Procedures  and Guideliies suggest  that several  risk extrapola-




tion models  should be  utilized when  assessing  a carcinogen




[F.R.,Vol  41 : 21402-21405(1976)\ .  To  ob.ain  some  insight  on




how  the  risk estimites  would  differ  under a  different model




and  exposure assum ) tions , the log-probit  model was also




employed.  The results  .re shown in Appendix  C.






     At  present,  there  , s  no clearly  defimd  rationale




within  the Agency  for  selecting or eliminating data  in




multiple concentrat ion-sex-species-tumor  type experiments.




The  primary cancer dat;  utilized  in  this  p, per is confined




to hepatocellular  carc'noma in male  mice  cited ;n NCI's




January  1976 progress  report, which  is  the iame data  set




selected by CAG  in its  kepone ant-bait  ana'ysls.   CAG chose




this data  set because  it  showed the  most  pronounced  Kepone-




related  effect.   In a  slight  departure  fror  CAG, both




                           4

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concentration  levels  of  Kepone were  utilized  in the slope

estimate.  This gave  a more efficient  estimate than CAG had

obtained using onl   the  high concentration.


      The  functional  form of the probability  of an animal,

fed x ppm  of a chemical  in its diet, developing a tumor over

its lifetime using  the "one-hit" theory  may  be expressed

a s


                                 -6x
                  P(x)=9+(l-0)(l-e   )                   (1)

where  6  is the probability of developing the  tumor due  to

causes other than the deliberately-added chemical in  its

diet and   Bis  the unknown "slope"  of the "one-hit" model  to

be estimated from observed data.


     Fitting this model  to the data  by non-linear weighted

least squares, the  result shown in  Figure   (p.19) is

obtained,  where   6= .1634 and $ =  .0791.  The probabi-

lity of  developing  a tumor due to  Kepone at  x ppm in  the

diet is  then expressed as

                           *    -.0791x
                       P(x) = l-e                        (2)


and is also  shown in Figure 1.  In  Figure 2   (p.20) the

relationship at  the levels of expected hum,- n exposure  is

also illustrated, whict  clearly shows  that the response at

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this level  is virtually linear  so  that the dose  response

relationship may be expressed with virtuall • no  loss  of

accuracy as

                          *
                      P(x)   =  .079lx                    (3)

To use  this  equation for man  the  assumption  is made that

ppm in  the  diet  is an equivalent  dose for all  organisms

Thus, the value  for X is the  Average lifetime  pi>m  in  the

              *
diet and P(x)   is the probability  of developing  a  cancer

due to  Kepone  during th i entire life span of the individual.

This relations? ip, even assuming  equivalence between  life

span exposure  of mice and  man,  w < u 1 d only be strictly valid

for a cohort  of  men exposed  at  firth.  However,  as a  limiting

case an additional conservative assumption could be made

that exposure  has the same  effect  over any segment of the

life span.  Coupled with  the  linear dose retpon.se relationship,

this means  that  we need  only  consider man years  of exposure.

Thus, the  probability of getting a tumor  in  seventy people

exposed for one  year is  the  same as  the probability of  one

person  getting  a tumor  if  exposed  for seventy  years.

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           ACTION LEVEL?  OBTAINED  BY  CONSIDERING




                     RISK  TO INDIVIDUALS




      An  implicit assumption  in  the  establishment  of an




action  level is tlat  t' > e risk  for  any leve   below the action




level  is  tolerable.   This is tru;  since tie  foodstuff is




allowed  to be used  as long as  its  chetiica] content does not




exceed  the action  level.  Since  any  chemical  level has some




potential  risk associated with  it,  tie max imum  allowab1e




risk  is  established  by  the choice  of the  action  level




Conversely, given  a  saiety factor  to account  for  inter-




species  sensitivity  differences  and  the calculated risk,  it




is  possible to establi'h a c or r es pond i np,  action  level.  It




should  be  emphasized  that such  a  determination  depends only




upon  the  esta lished  dose response  relationship  and hypothe-




sized  food in ake.   It  should  also  be emphasized  that the




actual  risk is not  necessarily  equal to the  risk  associated




with  the  action level and, in  fact,  will  usually  be less




than  the  maximum due  to  the  fact  that ac tual  chemical




residues  are less  than  action  levels.







      EPA  has recently developed  information  to  be used




in  safety  determinations for calculating  food intake  of




various  food stuffs.   This information was culled from





several  USDA studies  and indicates  that the  average weight




of  solid  food eaten  per  day  per  individual in the U.S.




is  1,930  g.  If aj  is defined  as  the action  level and




wj  the  weight in grams  eaten per  day per  Individual of

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the jth types  of  food stuff  thei  the statistically  calculated

risk of developing hepatoe el 1 ular  carcinoma  in  the  lifetime

of an  individual  who eats wj  grams of the jth  food  source

daily  is
                                         -5
                   = ±07' Ixaj wj  =  4.098x1 f) x aj wj         (4)
                        i 930
where  a j v j  is  the maximum  number of ppm in  the  diet from

       1930

the jth  foodstuff.


    Although in specialized  circumstances one might be

interested  in  the risk due  to  a single item,  it is more

likely that the major concern  would be total  exposure

through  the entire diet.   In this case, the  total  maximum

calculated  risk to exposure  of  k different  food stuffs would

be  expressed as
                                  k

                 P = 4. (98x10 x ^.^ aj w]                  (5)
      From a strictly  t ox io 1 og ic al viewpoint  the action

level  for each foodstuff  should be the  same  since a standard

unit  of  one foodstuff  las  the same effect  as a standard

unit  of  another.  However,  if the nutritional, economic,

and/or recreational value  of one foodstuff  is greater than

another  foodstuff,  then  it  is desirable  to  directly incorporate

this  information  in establishing that action level.  For  each

potential action  level  an  implict risk-benefit ratio exists.

                            8

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To maintain  the same  r j s k-l> ene f L t  ratio for all  foodstuffs,

thus insuring  an equitable treatment  of all sectors  of the

fishing  industry, the  ;ctLon levels  should he made  proport-

tional  to  the  value per  unit volume vj  so that


                                       k
                                      V
                P =  4.0 ''8x10  xa  ^<\  v j wj                 ( h)
                                      .1=1
where  aj  =  av j . This  implies that  if  a foodstuff  has value s

times  as  great per  unit volume as  another, a risk s  times  as

great  will  be  acce 'ted,  and  fie same  risk-benefit ratio will

be kept,  where it is  assumed that  the calculated  risk or

risk-benefit ratio  has  been  fixed.

     To  be  specific,  consider the  case where it  is  assumed

that all  foodstuffs have  equal value  to the individual.

In this  case aj =  i,  j=l,2,...,k  so  that
                                    k
                            -5
                P=4.048xlOxa       w]                      (7)
In  the  c ise of Kepone-"ontaminated  seafood,  the  term

k
\x wj=W  is simply  the  total daily  intake of  all  forms of

3=1

seai'ood  from the  potentially contaminated  ire a  in  grams.

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     In j-, eneral, given  P,W, and a  safety factor ijj ,  the action

level can  be  obtained  from the relationship


                               5
                       a= i^PxlO / 4 . 098W
                                                       (8)

The relationship between P and a  under three different

assumptions of total  seafood intake,  W , listed  in  Table  1,  is

shown in  Figure 3  (p.22) and Table  2, for the  case  of no

safety  factor, which  means that  ty =1.
Table  1.   Posible  Levels of Seafood Consurption to be
Utilized  in DC term  nine  Action  Levels	

    W
Grams/Da '      Ra t i r n a 1e

  29           Average  U.S. seafood  intake,  lower bound
 490           Average  intake from  animal sources, practi-
               cal upper  bound
1,930          Averap, e  total  food  intake, a'so lite upper
               bound
Table  2 .   Potential  Action Levels  for Different  Levels  of
Risk  and  Seafood  Consumption	

                          W
                        G/Day
                    29             490          1930
_p
-2
1x10
-3
1x10
-4
1x10
-5
1x10
Act
8.415

.842

.084

.008
ion Levels
.498 .126

.050 .013

.005 .001


                            I f)

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         ACTION  LEVELS OBTAIN ED _BY CONSIDERING
                RISK TO TOTAL  POPULATION


     If N(x)  Is  defined as  th'  total number  of  individuals
                           *
exposed at  le  el  x , and P  (x),  the probability  of  an induced

tumor at leve   x  i; defined  as  in equation  (3),  then the

total e>pecte   number if  tumors  in the exposed  population

producet is
T =   ^^  x  P  (x) N ( x ) =  .0791   / ^ x N ( x )            ( 8 )
      allx                        allx
The term  / ^  xN(x) is simply  the total exposure to the entire
          all  x
exposed human population  and  remains the  same  no matter how  the

Kepone  is distributed vithin  the population  since the amount

of f Lsh eaten is fixed.   Therefore, under  a  "one-hit"

hypothesis,  exposure patterns  do not affect  the total tumors

produced  within a population.


      If a population is exposed  to k different foodstuffs,

each  with a  total weight  consumed per day  of  Wj  grams, with

an actioi level of a j associated with it,  then the implied

total calculated number of  tumors that could  be expected  to

occur is
               T = .0791 ^  aj Wj                      (9)
                   1930  j=l
                           1 1

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      The  total weigits eaten per day  of the  items that  have

set  prescribed actim levels are shown in Tnble  3.  Fish

meal  is  also considered due  to the  possibil  ty  that it

will  enter int>  the human  population  via th   food chain  from

chickens.   The data and the  basis  for exposure  estimates

from  consuming Chesapeake  Ray seafood is given  in Appendix

A.   To  estimate  the imp  ted  c ilculated number  of  tumors  that

could occur for  the present  action  level, Aquation  (9)  is

applied  to the derived weight; and  action levels.   The

results  are sh>wn ii Table 3.
     Table 3.   implicit Calculated Number of Tumors
     Under Present Action Levels
Item

Fin Fish

Crabs

Shellfish

Fish Meal

Total
Weight e ten by
population per day in kg

3.64H

.72

2.32"

1.281

7.985
4
x 10
4
x 10
4
x 10
4
x 10
4
x 10
Present
action lev el

.1

.4

.3

.1


Implied calculated
of tumors produced
per lifetime

149.6

119.2

286.4

52.5

607.7
number
per year

2.137

1.703

4.091

.750

8.680
                             12

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Thus,  if  all  of  the commodities taken  from  the Chesapeake




Bay had residues equalling  the current  action levels,  the




one-hit model  Implies  that  approximately  8.7 hepatocellular




carcinomas  per year would  be the calculated risk.







     In d< ing  a  risk-benefit analysis,  it  is considered




preferable  to  rela:e  the  risks and benefits as close  as




possible  to the  saie  population and  if  possible to cast  both




costs  and  benefits in  terms o  health  units.  Taking  this




approach,  one  measurement  would be to  relate the number  of




people  in  the  U.S. that  would be supplied  with their  ace urns-




tomed  intake  of  food  fiom  animal sources  for a year  to each




hepa toe e 11 ul ar carcinor a  prod iced.







     To obtain the total  number of people  whose animal  food




needs  are  supported by  Chesapeake Bay  seafood, the edible




weight  in  kg/day obtained  from fin fish and shellfish  is




summed  up  and  added to  this is the total  weight of edible




chicken obtained from  the  use of fish  meal.  This number is




then divii'ed  by  .41 kgm,  the daily animal  food intake  of an




average Anerican.  "he  conversion ratio  from fish meal  to




edible  chicken is  .385  x  .4 = .1>4,  where  .385 is the




fraction  of total  chicken  weight that  is  edible and  .4 is




the energy  conversion  ratio from feed  intake to weight of




chickens.   This  number  is  then divided  by  the total  tumors




produced  per  year  under  the assumed  action  level to  obtain




the required  benefit/risk  index.  At  present action  levels




this amounts  to  16,226  Americans supplied  their animal food




needs  for  a year for  every hepa toe el lul ar  carcinoma  produced,







                           I 3

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           To set  action  levels  on the  basis  of  a' sumod  risks  it

     is  'lecessary t i specify  the  total  calculate) number of

     tumors and  the weight of  each foodstuff  and the  relative

     value per unit wel| ht of  each foodstuff.   In Table 4  the

     action le\els for   everal  specified condiions  are shown.
Table 4.   Resulting Action  evels In PPM Under Different Risk/
Benefit  Assumptions
Allocation of value
in same mannei as
relative price at
docks j de
Food
Stuff
Fin fish
Crabs
Shellfish
Fish Meal
Price/lb
.54
1.80
.81
.12
Relativ e
v alue
4.50
15.00
6.75
1.00
I/year {
.017
.058
.026
.004
- Numbers
J.68()/year
.151
.502
.226
.033
Same Vn ! no
Weight
of Expected
I/year 8.
.021
.021
.021
.021
' Un i t

Tumors -
)80/year
186
186
186
186

I'resen t
8.680/year
.1
.4
.3
.1
          For  example,  to calculate  the action levels for  the

    case  where  the rela ive values  are equLvnleit  to their

    dockside  price and   >nly one tumor per  year  is  allowed,
                                   14

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70 tumors per  life  tirne  are equated to the right  hand

side of equation  (10)            ^
                         T= .Q791a  >v vjWj              (10)
                            1.93    j-1
giving the  result:   70= .0791a [4.5 x 3.648+15.x.727+6.75x2.329
          4
+1.281]xlO  71.93,  solving  for a, a= .0039-.004  which  sets

the action  level  for fish  meal. The other  action  levels  are


obtained by multiplying a  by the relative value  of  the

foodstuff.  The  levels are shown in thi; third  column  of

Table 4.
                            15

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              ESTIMATED  RISK TO POPULATION  IN  AREA CONTIGUOUS TO
                    CHESAPEAKE BAY UNDER CURRENT  ACTION LEVELS

              If  the  Implied average risk rate to  any segment of

         society  is required it can be computed  as
P=
                                            ajfjWj
                                             N
                     .0791
         where  Y=  365  x 2.20+ x 1.930;  fj  is  the fraction of

         the jth  foodstuff that is consumed, by the specified  segment

         of society;  Wj  is the yearly  total  Intake in pounds;  and  N

         is the  population size for  the  considered segment.


              Under the assumptions  listed  in  Appendix B,  the

         following  table was created which  shows the average  implied

         risk to  an individual in the  counties surrounding Chesapeake

         Bay  in  Virginia and Maryland  considered separately.

Table 5.  Implied  Risk to Individuals in Chesapeake Bay Area
Location
Maryland (Bay)
Virginia (Bay)
Population Size
1970 Census
2,052,178
1,004,580
Fraction
consumed
Fin fish
.57
.57
of total
in location
Shellfish
oysters clams
.72 .40
.72 .40
Ci abs Calculated
249.01 =
.53 2,052,178
.53 92.94 = 9
1,004,580
Risk
1.21x1
.25x10
                                      16

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                EFFECT OF  KEFOME RESIDUES  IN BLUEFISH







      In  order to estimate  tie actual  harm from Kepone  in




foodstuffs  it would be  necessary to devise a comprehensive




sampling  plan for both  commerical and  sport fisheries.   At




present,  the monitoring  efforts  undertaken by the states  of




Virginia  and Maryland an!  PDA have been  concirned more  with




evaluating  whether 
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     If  it  is  assume!  that  the two  cited monitoring
       A
studies   give  a sample  that is at  least roughly  repre-

sentative  of  the population and  that  present conditions are

stable and  will continue  into the  future, then  a  crude

estimate  of the effects of  citing  bluefish can  he obtained.

In Table  6  the number  of  t u IT • > r s  calculated from  the  weight

data found  in  Appendix  B,  using  equation  (7)  ar<- shown

where  the  observed  average  residues levels o>  .179 for

Virginia  and  .059  for  Maryland are  substituted  for the

action lev-'ls. The  numbers  in parentheses ar   the weights  in

thousands  of  kg for  each  catagory  ii  edible  'eg/day.
Table  ii .   Lifetime  Production  of  Calculated  Tumors due  to
Co Tsumption of Bluefish
Met

Spo

h

r

od of Catch

t


Mar
20
(H
Commer ical

Tot

a

I
(
21

yl
. 5
.4
. 7
. 2
. 2
Loc a t
and
4
9)
0
9)
4
ion
Virgi
22.
(3.
6.
( .
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5
Due  to catch volumes, th1 major  part of  the  total  risk  comes

from the sport  fisheries, and  even though  levels are much

higher in Virginia  the total  risk is roughly  comparable  for

both states.  The  total effect  of bluefish  is estimated  at

the  production  of  5('.05 calculated tumors  pe   lifetime  or  .72 per

year.
*Data sent to Jack Blanchard  by Max Kisenberg, Maryland
Department of Health and Mental Hygiene,  August 6, 1976.
Appendix dated  June 30, 1976  from VJ. J.  llargis, Jr.,  Direc-
tor,  Virginia Institute of Marine Science1  Subject: Mounting
Kepone Levels in  Chesapeake  Bay Fish.

                             18

-------
       PROBABILITY OF DEVELOPING HEPATOCELLULAR CARCINOMA

              DURING LIFETIME AT SPECIFIED DIET LEVEL
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-------

-------
AI  PKNDIX  A

-------

-------
                       APPENDIX A
                     LIST OF  TABLhS
                                                          Pae e
1   Total C o 'ii m c ; c Lai  C a t c h :   V < • 1 u e  a n d
   Pounds  in L iv e Weight,  State  of
   Maryland, 1^74 and  ! 9 7 r>	,	  26

'   Total f.ommereia   Catch  in Value and  Pounds
    i n  L Lv e  We i  p, h t , S t a t e  o f  V L r t', I n ! a ,  1 9 7 4
   and  1975	,	  27

3   Total Catch  and Fstimated C o n R u   Total Catch  and F, s t  mated Consumption of
   Selected  Fish  from  Chesapeake  Bay  an<' its
   Tributaries  for Maryland......	  31

6   Estimates o'  Edible  Pound s a nd  V a 1u e s of
   Fin  Fish  and  Shellfish  f r o;>  Commercial
   Fishing  in  the Ches, p e a k e "> a y  and  its
   Tributaries  during  1 9 7 S	,		  'M

7    Estimate  of  Edible  I't) u n d s  ind  Value  of
   Fin  F i s <\  and  S h e I 1 f : s h  f r c n  S p n r I   !•' i s h i n j;
    in  the  C h e s a p e a K e.  B a y  a n d  its  'I r i h u t a r i e s
   during  1974	,	  35

H   Total  ' o u n d s  of M a n h a d d r-1 n and  A ] o w i v e s
   Caught  in the  C h e s a p e a k e  R a v  by R o >; i on
   and  Estimated  Pound-; of  Fishing, 1 9 7 S	  37

9   Av e r a g e  Prices for  Chesapeake  Seat > o d
   Species,  1975	  59

-------
                       APPENDIX /






   PRODUCTION AND  CONSUMPTION OF CHESAPEAKE  BAY  SEAFOOD









     As part of  the  effort to evaluate  risks and benefits




for this study,  it  was necessary to estimate the total pounds




of edible seafood  produced by the Chesapeake Bay for a given




period of time.   Consumption was estimated  frem  production




or catch and by  conversion of live weights  into  edible




portions. Consumable seafood products  were  obtained from




three sources:  commercial seafood industry,  sport fishing




and indirect c ons amp t iori, whereby some  fish  are  used to




make fishmeal,  which is fed in small amounts to  broiler or




fryer ch ic kens.







     The amount  of seafood in pounds from comercial fishing




was listed  for  1975 s the most recent year for which annual




data was available*   A survey of recreational fishing was




conducted in  1974 by the National Marine Fisheries Service,




and tbls data  was used to approximate  the amount of fish obtainei




from sport  f i s h .1 n g -.







     The risk  anal/si  In this study was conducted for




a  "worst" case1*  situation.  Tho data presented  in  this




appendix was  consistent with  worst  case assumptions.  For




example, it was assumed that  all fish  caught are consumed,




and no  allowance Is given for spoilage.

-------
     The  Chisaprake Bay  Bipports a  large commercial  seafood




Industry.   In  1974 an-1  19 ; 5 the Bay  produced approximately




6% of value  snd 12Z of  poundage of  total U.S. commercial




catch.   The  Bay produces  approximately j<'<£ of the  nation's




non food  fish  used in  the  production of fishmeal.   Sport




fishing  is  an  important  itdustry.   For most  species  of  fish,




total catch  from sport  fishing exceeds commercial  catch.







     The  data  used ti  approximate  pounds of  Chesapeake  Bay




seafood  in  elible  form  and  corresponding values  is presented




in the  following tabl ?s.   A description is provided  for




each table.







                    Tables  1 and 2







     These  tables  lift  total  pounds  (Jive we;ght)  and




total value  (docksidc  or  exvessel)  for the most  important




fin fish  and shellfish  caught  for  che states of  Maryland  and




Virginia  in  1974 and  1975.   The data include the Chesapeake




Bay and  its  tributaries  and the Atlantic Ocean  for «acli




state.   Revenues to  Vvitermen  exceeded $22.4  rillion  in




Maryland  and $25.4 million  in  Virginia  in 19 5.

-------
Table 1.  Total Commercial Catch:  Value and
Pounds in Live We Lgh^,_S^ateL Q|T Nary land ,1974 and_1975


Food fish
j>£e£ie_s_
Fin fish
Bluefish
Striped Bass
Grey Trout
Croakers
All other
Total
1974_
Ib
(000)

574.8
3,567.0
411.8
120.4
2,488.6
7,162,6

$
(000)

$46,0
948,0
54.5
18.5
50.4
$1117.4
1975
Ib
(000)

270.9
2,722.2
891.8
639.1
3192.9
7,716.9

$
(000)

$25.0
1,064.2
79.3
52.8
715.9
$1,937.2
Shell fish:
Crabs
Oysters
All other
Total
Non food fish
Grand Total
26,483.0
18,284.0
7,859.8
52,626.8
**
7638.2
67,430.5
5,085.0
12,707.6
2,890.0
$20,682.6
188.6
$22,418.03
25,900.0
15,953.5
6,755.8
48,609.3
7,194.9
63,524.5
5,145.5
12,947.2
2,245.3
$20,338.042
190.3
$22,465.60
 * Includes Chesapeake Bay and tributaries and Atlantic
   Ocean.
** Includes ale\'ives, gizzard and shad, hickory shad,
   menhaden and sharks.

Source:  U. 5, T'ppartment of Commerce, National Oceanic  and
Atmospheric Association.  Current Fisheries Statistics
No, 6952:  Maryland Landings, December, 1975, Washington,
D.C. (February, L976).
                                   26

-------
                     •. .'if ic ial  f'.atch  in  Value  and Pounds
S r '-• i } i i ii h
      * •*
                     '!i-  'L ,Y,iLS.^nl£-»_ J 2Z^ an!_JLLL!	._
                           19; •'-
                    .,663.2    ^4,656,4
                    '• v 7 3 7 . 7     4,844.0
                    S 2 3 7 4 . 0    i 0 ,1 9 4 . 0
                 i  . :- ,774.9   $19 ,694.4
                                                         1975

::j-;;f i;<
F-i i,: i
B !, a c f I
St ; I :•,(•
C, r e ;, T
" » o a V e
', > ''. '"• t
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s
i r ! .
sb
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* s
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, ',
r. n

, i
5
"• ' 0

, 2
? ,5


5

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1 / , 3
64.2
6?. . 'i
• i , '
3 5 , l
nl . 3

s 2
' f
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2
2 . 6
4 ,1

hi
i *
ft ' »
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85 .

8
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8
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1 i , 4
25 ,8

84.
29.
89.
20.
24.
76.

9
1
8
9
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4
$
( 0 (i 0 )

$246.
h 4 ; i ,
554.
51 2.
2,977.
$4,932



2
'.'
1
9
9

                                                 35,562.0      5,399.9
                                                  ', „ ^ 4 4 . 0      4,399,9
                                                 43,008.8      0,433.9
                                                 84  114.8    $19,433.9
U n v  1 n <3 P I f i f r,
T o r » 1

r. r ••, ncl  T_^ '  a i ^_

      include
•v *    1 n c 1 u d •  .
* '-'' *   1 n c 1 u'i -'
      s T? d  s h F '
                   ^'« ,576.2
                    '4,377.8
                   »8 ,954.0

                   '2 ,230.2
  $921.8
   120.5
 $1042.3
JAii211  1.
 48,106.5    $1158.9
  6,825,6      161.6
 54,932.1     1320.5

164,923,3    $25,486.2
                  ;> a p e a k i -  Bay and  tributaries a n (  Atlantic Ocean.
                  •i'd,  so It  and peeler blue  crabs.
                  • i i' i c e s ,  g  zzard  shad, hickory  ihod,  manhaden
           '-   c-  '.- p.» r t jn .• n t  c f Gommc re e ,  NdtK-ual  Oceanic
           and  A i id o H p h e r i c  itsociation.   C u r r c n L  Fisheries
           S •• a ?. : - i  i c s  t J o „  6 ' j 3 :   Virginia  Landings, December
           1 n 7 r.    Wa s i, L n g t o i ,  D . C .  ' F . b i u a r y 1 9 ? 6 ) .
                                  2 7

-------
                   Tables  3,  4, and 5




     In these tables  pounds  of fin fish and  shellfish are




listed by commercial  and  sport fishing catches.   The  estimates




from commercial  sources  weie  obtained by  summing  up the




respective monthly amounts reported in the Maryland and




Virginia bulletins on current  fishery statistics, published




monthly by the  National  Ma Lne Fisheries  Service, National




Oceanic and  Atmospheric  Association (NOAA),  U.S.  Department




of Commerce.  Data  is cate;>orized  by  body of water.  Therefore,




it is  possible  to  lerive t'le amount of catch exclusively  for




the Chesapeake  Bay  and its tributaries.  Fin  fisli are listed




by total  liveweight  pounds for each category of fish,




whereas oysters and  clams ire  listed  by  pounds of edible




meat.  For  fin  fish  and  bl'ie crabs it  was necessary  to




convert live weight  of fis'i  into  edible  weights.  The




conversion  factors  (edible weight-live  weight) were  obtained




from  Lee  J.  Weddig,  Executive  Director  of the National




Fisheries  Institute, Inc.  The conversion factors  are  one




third  for  fin fish  and one tenth  for  blue crabs.
                               28

-------
TnM->  1,,  '
F | -' \ ;<  ( r n **|
                             K H t I mated  Consumption  of  fie let ted
                             jMid  its Tributaries
   1 i  • !•.
   (•;}••
i: i ur t f L
( .J t < (i
"live ve
2,23
2,87
33
i t J 0
1 ,':8
M!
«. , , o
v 9 , n
2,17
3 S
i "' , --, U
3 , V 4
i|
8
1
9
3
7
7
6
0
0
8

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9 '
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9
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0
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C o n s ujryj t i
b edible w
74
95
1 1
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C4 '/
y £,
20
2 s 2 3
2 . l> 2
2,17
'Si
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2
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5
2
8
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3
L.
0
00
A- 0
                b i 1
 -c (i j ) »
 •" <   '•,  ,
i  I  T! r  i
                            . i  > ,•! 1 1 h .
                            i ' f> ')  catch a i e  I" r oin  j  i  •) I H  s u i v ^ -
                            ( S e  -  H M b r e •  ,  V. ,  I ,  ;i u i v  (. y  i - f  K e t  i e a
                            ! t c. i    0 u r r «' i, I   !•' i s h ;• i i e s  - I a f i s (. j > s
                            The  fits   fish  classification  i n c 1 n d e
                                c i I p ij >l  bass,  spot,  and  1 1 o ;>
                                p  c r a b (•; ,  o y s t e r s  and  h a r d s ! i
                                                                   ( wt> a k -

-------
Table 4.  Total Catch  and  Estimated Consumption
Selected Fish from  Potomac  River, 1975
                        of
Food fish
Commercial Ci tch
Bluef ish
Croaker
Flounder
Grey Trout
Shad
Striped Bass
All Other Fin fish
Blue Crabs
Oysters
Clams
Catch
(1 b liv e we iRht )
620,786
41 ,658
77,825
181,559
144,930
840,105
1,906,863
2 ,068,417
219,711

                                                    Consumption
                                                 (Ib  edible weight)
                                                       206,929
                                                        13 ,886
                                                        25,942
                                                        60,520
                                                        48,310
                                                       280 ,035
                                                       635,521
                                                       206 ,842
                                                       219,711
Non food fish

   A1 e w iv es
   Menhaden
16,251,530
3,413,633
* Ex eludes Tributaries  of the Potomac River
                              30

-------
!'•>;.!<    .       !  '»i   ',   •  .•• i  -  '.    .. u-<   i    •  1 ,!!;> u -1   :  •' a'' ..in jj t J mi   u i

-------
                             Table  6







     This table summarizes  commercial food fish data  from




Tables 3 to 5 and  includes  pounds  of edible fish by  species




for the Chesapeake  B; y  and  its tributaries for Maryland




and Virginia and separately for the Potomac River.   For  the




entire Chesapeake  Bay,  approximately 30.75 million  Ib of




edible seafood  products were produced from commercial




fishing.  Almost one-half  of this  is made up of Maryland




oysters.
                             32

-------






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-------
                        Table 7.

     Th€' National  Marine Fisheries  Service, NOAA conducted

a survey of matins recreational  fisheries for the Northeast-

ern U,,S, for  1974.  Maryland and  virginia were also  included.

The result'.- of  this survey, currently  unpublished, were

provided to EPA by the National. Marine Fisheries Service.

Data on sport  fish catch was obtained  by telephone,  mail  and

household  survey.   For each state  the  following species  were

separated  individually; bluefish,  white perch, striped bass,

spot, and  weakfish.  The other categories included all other

fin fish,  hardshell clams, oysters,  and crabs. According  to

Ernest Mabrey  (NOAA), the senior  author of the study,  87.5%

of the  total  reported weight of  catch  was from the Chesapeake

Bay.  The  survey provided percentages  as follows:


Species of  Fish                      Percent of Total
                                      Finfish by Weight

Bluefish                                    47

White Perch                                  9

Striped Bass                                10

S P o t                                         5

Weakfish                                     8

All Other                                   21


Since the  percentage breakdown i;f.  v, rights for shellfish  was

unavailable  at  t.ho time of thin  vr/ I t ing , it was assumed

that crabs made up 80% of shs?1lfJsh, and oysters and  clams

each account e d  f o r 101 f o; a total  of  100%.
                              3/4

-------

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-------
      In order to assign a value  to  recreational benefit

  from fishing as distinct from  the  value  of fish for food,

  the total expenditures for  fishing reported for each state

  was proportioned out for the Chesapeake  Bay.  Then, the

  total weights of edible fish by  fin fish and shellfish

  categories were used in assigning  weights for  letermining

  per pound of edible meat.   The values are reported as

  follows:
State
Ma ryl and
Virginia
Fish
Category
Fin fish
Shellfish
Fin fish
Shellfish
Weight
Dist r ibut ion
86
14
80
20
Edible Pr iducts
$ per p >und
$2.4 >
$7.8 5
$2.37
8.1t
Total
Expend itur es
$35,230,000
$ 5,933,000
$12,231 ,000
2,976,000
                            Table  8

     Manhaden and alewives  are  the  major ingredients in

the production of fishmeal.   The  Chesapeake Bay, an important

producer of non food  fish,  supplies approximately 30% of  the

nation's total production.   By  weight, fishmeal is approxi-

mately 21.005% of the  original  live weight of fish.  This

percent was obtained  from  the ratio of the contribution  of

manhaden in fishmeal  to  manhaden  catch. The ratio was:


   .21005 = 199.2 thousand  short  tons of manhaden in fishmeal
            1,896.7 million pounds  of raw manhaden


The figures represent  total U.S.  production and

are averages  for  1970-74.

-------
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-------
                       Tab la  f)






     This  table  lists prices  for  each species.   The  prices




are dollars  per  pound for b o f" h  live weight and  edible fish.




Live weight  prices a r >.> obtained  by dividing  total  revenues




by state  received by watermen  by  total pounds  of  catch




for 1975.   This  procedure is  also used for the  category,




"all other fin fish."  The average live weight  price column




is obtained  by weighting prices  by pounds of  fish  produced




in each  state.  Average  prices  for edible portions is




obtained  by  dividing live weight  prices by the  fraction




of edible portion to live weight  of fish.







     In  the  analysis, the prices were used to  derive relative




values  of each seafood product.   These relative prices were




used to  rank tVie seafood products by value.   The study




should  not be interpreted as an  economic  impact analysis.




However,  it  could serve  as  a basis for one.

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Table 9.   Averages Prices  for  Chesapeake  Seafood Species,

1975.
Species
Bluef ish
C roa ker
F 1 ound er
Grey trout
(Weakf ish)
Shad
S t r iped Bass
Ma ryland
.09
.08

.09

.39
Virginia Average
.07 .08
.11 .10
. 284
.14 .12
. 266
.48 .44
Av er ag e Price
.24
.30
.852
.36
.798
1.32
(Rock)

All Other       .22            .22            .22              .66
Fin Fish Used
as Food

Weight Price                                 .025
of Alewives
and Menhaden

Fishmeal                                                       .119
* Prices  are dockside  or  exvessel equivilent.

      Source:  tl.  S. Department of Commerce,  National Oceanic
and Atmospheric Association.   Current  Economic Analysis
1-26,  Industrial  Fishery  Products:  Market  Review and
Outlook,  NOAA JFPOA 26, Washington, U.C.  (February 1976).
                                 39

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APPENDIX   B

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                      APPENDIX  B
                     LIST OF TABLES
No.

1   Population by  Cities and  Counties Contiguous
    to  the Chesapeake  Bay  in  Maryland and
    Virginia, 1 9 7 0 ....<•...... t ........ ,..„.....,»

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                         APPENDIX B

         ASSUMPTIONS USED TO  COMPUTE IMPLIED  RISK
                  TO A GIVEN  POPULATION

                          In  the  equation,

                          P=  i*   aj fj Wj
     The following  variables  are discussed:

     aj = action  Levels for  species j

     fj = percent of total  production of  species j consumed

          locally (cities  and counties  contiguous to  the

          Chesapeake Bay)


     Wj = Total  pounds of  species j produced in the

          Chesapeake Bay  annually  (based  on  1975 data)


                      Action  Lev el s


     The a : t i o n  levels chosen are  the ones that are  currently

in effect for  shellfish,  crabs and oysters and are:
                •j   (Fin  fish)    =0.1
                 1
                a   (Oysters)     =0.3
                 '>
                 £.
                a   (Clams)       =0.3
                 1
                a   (Blue  Crabs) = 0.4
                 l\
                           42

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                     cropoi I  ius    M   Fit: l>   (  HtBunit'sj   Loral I;'

               f \i  ., , .: ,'   f  1 i   pci .-  t is!   of   :" p e e ios  c o n n !• inf!  local  1 y  are;

                       i      { i: MI  i I  r-li)           1) /'
                         1
                       f      nr-f Sjr err,)       -•    722
                         >
                       i      , f I aws)           ••    40 2,

                       !      ( H i. u L   f i a h  ;>  )  -    55 %



                         f.  .  ,1 V cl o «'>ii'.  ioi   M < ,-• i c r si -   ' l.ini'i,  -'ii','<   c^al-s  are

c .>!<<;« u i »•:;   '•'    !]!on  of  thr=   UnMrci  '". : -i i •"" a  , -'- '   rroduc-

• ; ' •"'   'ir,';   >i !       j i, • i ,..,,  aii"  ci-( i1  i. ii  1  i^i.ifn  '"    ;   "4 n <\  '•'•   For

                                                                 '•'/
                     . ,•      -i  c i ,il  •.    <  e;:p,'. ;• i-s p i y „ •     1 ;.<   :•'•-.  ( •  i    )  i-r cent

                         ',•>,.,-(,  t li t   i" a I  i c  I •>{;   p i MC r ii '..  ;•  '"i •'    >• ; .1  t- j n r f

                     •':   '•;'•! h ii  t t r ii   s'f r ••   i. u i   r r po i  f r.;  i   ;   . i n   fish.
                                                                    ">   '"
                 '" ';   '• r   ".>'•;;<(«>  v> i:.  in,,uli  n i   total   f  in  '   * ''<   • -  is JMMM ]»t 1 on


      >u1 '- I f 1 y  ; >•-   •:•,-,•!,i i  pusn.St,  u !   ( '. n   f i -•( i;


       !   :  '  '     !  -    -  •-• •  sfc,e   !".• i   f  IK  '    Mfh   :', i !


                 ll< '   f <>!•;! i   |»o p "I ] ;i I  Ion  • :    tli,'   f" h f


(Tcf>  Tf'Mf  M,     ! •-.   ubtatn   the  .!F-nfr;-d  prr.p'---'  i--     "  lii •:


t. r o" *   ('••-  T" •• i   ;'.   i " 'l •• ,    in   (urn.,  f ii i  5,   (I»--fis«'    -  •   •    '   :,r.'-i\  to


•   '"" -• '        ;        ,  ', .  '    \: •> !, ii ,   Hi'UJtl'.il   ,111(1   (.'lllfc'l
                            f   c< i. P !  i i  I l h r     .   '•    I  i -.  i  I m i n a •  •,   !•' I nd ! tip. H ,
                           i,- l   S >i i'  i ',•> .1  t   C  ! i  i !; i  ,< ;    "j i  .'     N a ! i  on n i  H a -  i n c
                            „   N a r i o n a  I   Oo >  ;< i> f  ••   t mf  A i m t, - .j  hi- r t r   A:, s .ic L a
                            ,n c 111   C! t"   C ninms- r r r -   '• f- a t i 1 r     ) .T ^   !'!.]>.

-------
Table 1.  Population  by Cities and Counties  Contiguous to
the	Chesapeake  Bay	in Maryland and Virginia,  1970.	
Maryland
     Anne Arundel
     Baltimore  (City)
     Calv ert
     Cecil
     Dorchester
     Kent
     Queen  Anne
     St Marys
     Somer set
     Talbot
     Balt imore
                                  Total
  298,042
  905,787
   20,682
   53,291
   29 ,405
   16,146
   18,422
   47 ,388
   18,924
   23 ,682
  620.409
2 ,052 ,178
Virg inia
      Accorr oc k Co .
      North  Hampton
      Landraster
      Midd]esex
      M a t h e w s
      Glouc ester
      York  Co.

      Isle  of  Night
      Hansemond

      Norfolk  (City)
      Newport  News
      Hamp ton
      Virginia Beach
      Chesapea ke
    29,044
    14 ,442
     9,239
     9,126
     6,295
     7 ,168
    14,059
    33,203

    18 ,285
    35,166

   307 ,951
   138 ,177
   120 ,779
   172 ,106
    89,580
                                  Total
                           44

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              Tgta_l  Pounds  Ed 1 b 1 e Seafood

     The following  list  of  figures are estimated pounds

of fin fish used as  food  from  both commercial and

sport fishing sour' es  by  species  and location.


                                  State
Spec ies              Ma ryland            Vi rg in ia

Fin fish             16,451,311          10,821,616

Shellfish
  Crabs               2 627,400           3,221,073
  Oysters            15 173,917           2,208,222
  Clams                 961,857             395,890


                Population Estimates

     The  >opulation  chosen  consisted of state counties  and

cities which are contigious to  the Chesapeake Bay.   The

population by counties and  totals for each sub region  for

Maryland and Virginia  are listed  in Table 1.  The populations

are 2,052,178 and  1,004,580 for  Maryland and Virginia,

respectively. Since  these populations are arbitrarily  small,

the resulting probabilities  of  development of  tumors  are

somewhat high.   However,  this  does provide a worst  case

est imate.

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A  ' P  E N I) I X    C

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                         APPENDIX  C.  FIGURES
N o .
                                                                   Pae e
1    R i <• ]' Hiulpj  Present:  Action  Levels  and  Assumed
     C n is s u m ji t: L i ' i * , , *   »...«»».! . ,,  » .... e ...........
                                                                     5 2
2    F o I n :   ' i , 1 1 c 1 1 ] , s t > .-I  T inn o r ft Under  Present Action
     L e-f c. I  •>  a n d  U n i  a t m  C o n s urn pt, ion...... ........ ...«.«» 5 3

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                      AFl'EtiDi;,  C



RISK  TO FOI'HI.ATTmi ^i S IJM ! N (:  VH: rKOBIT MODEL  AND DIFFERENT
                     KKPOSUK I1,  I'M f JT.RNS

      In th?  case  ••> i' kepone,  the utility  of using a  two

parameter  model  to e r, t i m a t o  ; i •: l>  is s ev e r e ] y  limited  due  to

the  n a L v r o  s < t"  L h e o h s. P r v <> d  animal  data.   Tie  response 1 ev e 1 s

are  extremely  close t o> rth^r MI  11:  rig any  estimate of  slope

highly sci i L i Iv e  to rardom  b i c i .•!;; ic a 1  error.   Also,  only  two

exposure  > ev e 1 n  ex st  v n i r h  tnikos  any  evaluation of  the

conformity  of  a  two parameter  model to  the data impossible.

Furthermore,   n  joint  probability  distribution of the  amounts

of  kepone  in   finfLsh,  < tabs, and  shellfish taken from

Chesapeake  Bay  .m i 11 e f  such  a distribution is  not

av a i 1 ab 1 e ,  a  series of  B o m o wh n I- arbitrary  assumptions

concernirij  consumption  were  nade  in order  to  estimate risk.

It  must b • •  recognised  that  tie risk estimate  d e r iv e d  from

this model,  is  sensitive to  thcr.c-  assumptions.   For  these

reasons,  it"  ir-;  f o 1 t  )-'=..   M >• r^'libllity of  the "log-probit"

method docs n o f  ' a<< r:  ;      •• •  ' • . J i- r of  r e a 1 i b i 1 i t y  as that

possessed  by  t !• •"•   " : • r\ c -• i ' '  : ' ' I u a t i o n .

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    .        The  expected number  of  tumors under  thi  log-probit

h. , ,    model may  > e  expressed  is:

            T = X NSPS

       wher e

            p  - _J	   ..a
             s    /2iT -°°        fc  e   ~w
       is the  probability of  a  tumor in the  sth  exposure group  due

       to an agent  and Ns is  tie total number  of exposed people in

       the  sth  group.


       The  term  Xs  =  >\ a-W^s/1930 is the ppm  in  the diet of  the
                       0 = 1  J  J

       sth  exposure  group and  the other terms  are defined  below:

            m  =  number of exposure  groups
            a, B  are  the  intercept and slope  parameters of  the
                 log-probit  model
            k  =  the  number  of  different food  stuf's
            aj  =  action level  for jth food stuff
          wjs  =  grams eaten/day  of  jth food  stuff by a
                 individual  in  the sth exposure group

            In  order  to  evaluate cancer risk  using this model

       the  parameters a; 3 tnust  be estimated  and  an exposure  pattern

       specified.  However,  since the Kepone  data does not  lend

       itself  to  the  estimation  of  a reliable  slope for the  log-

       probit  modrl  it was  necessary to assume a conservative value

       for  the  slope. Following  the original  suggestion of Mantel-

       Bryan,  a  slope ofg=!  was  assumed.  An  intercept value a  was

       estimated  by  taking  the  weighted average  of the Intercepts

       obtained  from  estimating  the intercept  for each dose  level

       separately in  the heptacellular carcinoma male mice data

       set.   Proceeding  in  this  manner, the  log-probit equation

                      Zp  = -.437  + log x

       is obtained.
                                   49

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     Under the assumption that each  individual  eats  fish,




crabs, and shellfish in the same  ratio  as  they  are  consumed




by the entire population, an  implied  acceptable risk level




can be obtained given the action  levels and  the average




grams of seafood consumed per  day by  the individuals.  In




this case the ppm in th3 diet  x  is simply:




                 x » a~w/1930




where a" is the weighted average  of the  action levels of




each seafood type weighted by  their  total  consumption and




is the total seafood consumption  in  grams/day.   In  the case




of the present action levels  T -  .1857  and  the  log-probit




equation can be written as:




            Zp * - 4.454 + loslOw




and is shown is Figure  1.  The risk  of  any average  daily w




consumption can then be mathematically  or  graphically




from Figure 1.




     To calculate the total  number of tumors produced, it




is necessary to specify a consumption pattern or distribu-




tion of Chesapeake Bay  seafood.   The difficulty in such a




task is caused by the fact  that  most indivJduals are not




aware of the origin  of  the  seafood products they consume.




However, under the assumption that each individual eats the




same amount of seafood  the  number of tumors can be calculated,
                             50

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              ;t  i  i,ii-, uni  it  ion   o)    v  r r a ni s / il a y  i h i n  i.:r>},>-   flint
             !J  - 7.985 x  10'Ar



> q a, •  1   *  •    - ' •'   ," IIT b e r  of   p e o p ] o   t li a f:   R -i t   a 1 1  t h e   s r; a f o o d




 h ,'i v   -p •• f i i  i.»( .  r      T h r  f- n (- n 1   i" 11 m o in  1 •;   t x p r r- r, s n ij   n n ;






             ,=,       '-  -- '--  — -.         i                    e""    d I-
                   i'   '• i g n i <>   ..'   1 '»r   fi 1 i  •? ') 1 uef:   ot   w „    '•'»?   rn-i r-





                     i« li a t   t ii e   : r ti e.   rl  i r i  i ub t J o n   p a ;    ,  . '!   i  \   .  ^ u i :)





                      •if  i II ,1 ll   ';  , '' f) ii  i 'i i.l <•:!',,   The  Till.'  ll ;i,.i ',1 t' '   U!i ri •'





                    , ',  ; t i H> u t  I  i> n   i :i  -n n s t   i ikolv  H(>"ir  ^s  If, lit  -<1





                   >'   10  and   300   p, rdM,-;/   day   g iv  ing   'if.  a   ri mlu





                   • > i   A  , S 0 0   t  n in o r s ,.     M   must   HP  r '-in '• 'nh p T or'   that





                   'i   -i r i-   I) 1 g h  1 v   -'> p e '• i; 1  a f  I v e «

-------
RISK
P

 .5
      -4
 .3x10
       -7
 .61x10
Zp

0. w
 .159        -1.


 .067       .1.5


 .023        -2.
 .00135      -3.
-4.
•5.
                    APPENDIX C.  FIGURE 1  RISK UNDER PRESENT ACTION
                                            LEVELS AND ASSUMED CONSUMPTION
                                    a = .1857
                     -1.           0.           1.           2.           3.      log 10

                     .1   .316     1.  3.16     10   31,6    100  316    1.000    w gms

-------
'PENDIX C.  FIGURE 2 TOTAL CACULATED TUMORS UNDER PRESENT ACTION LEVELS
                               AND UNIFORM CONSUMPTION
     TOTAL
     TUMORS
      IN 103
      7.
      1.
      0.
a -.1857
T-NP        f 2p j_
N = W,P = i  J   eT
   w   %/2n -
                                  dt
                                                                         Iog10w
        .1  .316     1     3.16  10   31.6   100  316    1,000
                               10.000

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-------