EPA-650/2-74-059
May 1974
Environmental Protection Technology Series
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                                       EPA-650/2-74-059
N02  MEASURING  SYSTEM
                   by
       M. Birnbaum, and A. W. Tucker

          The Aerospace Corporation
       Electronics Research Laboratory
            Post Office Box 92957
        Los Angeles, California 90009
           Contract No. 68-02-1225
              ROAP No. 56AAI
         Program Element No. 1AA010
     EPA Project Officer:  W.A. McClenny

       Chemistry and Physics Laboratory
     National Environmental Research Center
  Research Triangle Park, North Carolina 27711
               Prepared for

    OFFICE OF RESEARCH AND DEVLEOPMENT
  U.S. ENVIRONMENTAL PROTECTION AGENCY
         WASHINGTON, D.C. 20460

                 May 1974

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This report has been reviewed by the Environmental Protection Agency
and approved for publication.  Approval does not signify that the
contents necessarily reflect the views and policies of the Agency,
nor does mention of trade names or commercial products constitute
endorsement or recommendation for use.
                                  11

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          NO2 MEASURING SYSTEM
               FINAL REPORT
                20 May 1974
              Prepared for the
       Chemistry and Physics Division
  ENVIRONMENTAL PROTECTION AGENCY
Research Triangle Park, North Carolina  27711
                    by
      THE AEROSPACE CORPORATION
      Electronics Research Laboratory
            Post Office Box  92957
       Los Angeles, California 90009

         Contract Number 68-02-1225
                  Authors
               M. Birnbaum
               A. W. Tucker

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            FINAL REPORT EPA CONTRACT NO.  68-06-1255


                       NO9 MEASURING SYSTEM
                          L*
                         TABLE OF CONTENTS
I.         Purpose and Scope of Program                                2



II.        Outline of Tasks                                              2


IE.       Characteristics of the LASER NO2 Monitor Prototype           3



          1.   Description                                              3



          2.   Optical Subsystem                                        3



          3.   Electronics Subsystem                                    6



IV.       Calibration and System Performance                           9



V.        Ambient Air NO~  Levels at Location of the Aerospace


          Corporation                                                 11



VI.       Conclusions                                                  11



VII.       References                                                  13



VIII.      Abbreviations                                                14



IX.       Tables                                                       15



X.        Figures                                                      18
                              - 1 -

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I.      PURPOSE AND SCOPE OF PROGRAM
        The purpose of this program is to develop a prototype "NO-
Measuring System" based upon a method originated at The Aerospace
Corporation.  A laser is used to excite the NO? molecules, whose
concentration in the atmosphere  is monitored by their resultant
fluorescence.  The method is of  great sensitivity,  i. e. ,  1  ppbv can be
readily detected.   The signal is obtained from the  NO- molecules and
therefore the method does not involve chemical processing or other
procedures which could compromise the  accuracy  of the measurements.
The technique has  been found to be free of interference due to the presence
of other gases.  The fluorescence methods can provide real-time,  automatic
monitoring of ambient atmosphere NO_ levels.   In short, the prototype
"NO9 Measuring System"  can provide the EPA with a unique means of
    O
monitoring ambient NO_ levels.
II.      OUTLINE OF TASKS
        The plan outline of work under EPA Contract No. 68-02-1255,
"NO_ Measuring System"  consisted of the following:  (1) procurement of
    LJ
major components, (2) design and (3) assembly of  the electronics which
•was tested with the Aerospace NO~ measurement system,  (4) packaging
of the NO- measurement system, (5) calibration and (6)  system performance
tests as specified  in the EPA contract.
        In the main, the prototype is  similar  to the systems operated at
the Aerospace Corporation.   Two important features  incorporated into the
system for  the EPA are: a He-Cd cw laser operating at 442 nm used to
excite  the NO_ fluorescence and  (2) the fluorescence chamber redesigned
for improved performance.
        The cw He-Cd laser is a compact instrument of approximately 10 mW
output at 442 nm.   Its virtues, compared to the cw argon ion laser  used in
                        1 2
prior work at Aerospace '  are  its compactness and relatively lower cost.
The output power of the  He-Cd laser (10 mW) is a factor of 100 less than
that of the  cw 488 nm argon ion laser output.  The utilization of the He-Cd
laser did,  of course, require careful design  to  obtain the required  perform-
ance accuracy.
                                - 2 -

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 III.     CHARACTERISTICS OF THE. LNMP (LASER NO,, MONITOR PROTOTYPE)
        1.     Description
        The appearance of the LNMP may be inferred from the photographs
 reproduced in Figures 1 to 4.  The arrangement of  the components is clearly
 visible in Figures 2 and 3 which show respectively the front view (with cover
 panels removed) and the back view (-with rear access  doors open).  For ease
 in operation,  adjustment and servicing,  the Laser NO- Monitor is configured
 of two subsystems (1) an optical assembly and (Z) the electronics and data
 recording system.  The optical subsystem assembly is easily identified by
 the He-Cd laser (Figure 2) and the chamber and photomultiplier tube housing
 (Figure 3).  A cooled photomultiplier tube in conjunction with a photon counting
 system is used to detect the weak fluorescence.  The relevant spectroscopic
 properties of NO7 in absorption and fluorescence have been described  in
                172
 earlier reports.
        2.     Optical Subsystem
        The heart of the LNMP lies in the optical subsystem shown  in the
 photograph of Figure 5 and the block diagram of Figure 6.   The Liconix Model
 401 He-Cd laser emitting at 442 nm with a nominal  output of  10 mW is used
 for excitation.
        A major technical problem in the design of a sensitive LNMP is that
 of background signals.  It is found that most materials emit a broad-band
 red fluorescence when excited by visible light.  Thus, scattered  laser light
 striking components  of the chamber results in a broad-band fluorescence .
 This  signal is observed when the laser beam is  introduced  into the  evacuated
 chamber (pressures  less 10 mtorr) and when the chamber is filled with
 pure  gases (such as He,  N?,  etc) which (as far as is known) do not  contain
 fluorescing species.   This signal, we refer to as background.  A development
 of an instrument with a low background signal is the key to the attainment of
high sensitivity.  Many of the details of the optical arrangement described
 below are used to maximize the NO_ fluorescence while minimizing the
                                   LJ
 background.
                                 - 3 -

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        In the LNMP developed for the EPA,  key features which have
 resulted in high sensitivity, low background and  ease of operation are:
 (1) development of an almost optimal fluorescence band-pass filter  in
 conjunction with excitation at 442 nm and (2)  the  liquid filter cells (Nos.
 1 and 2 of Figure  6) which  reduce background signals.
        The layout of the laser and chamber,  shown in Figure 6,  is dictated
 in part by requirements of compactness  and low  background signals.  Prac-
 tially all components which appear between the laser output end and the
 chamber end have been selected with a view to minimize the background.
 These are: (1) A Corning Glass Filter No. CS-5-61 to absorb the red  end-
 light emitted by the laser while transmitting  the  442 nm laser light; (2)
 442nm  mirrors which have high reflectivity at 442nm and  low reflectivity
 at longer wavelengths,  particularly in the orange-red spectral band; (3)
 aperture and lens to reduce scattered laser light and improve laser beam
 collimation.   This reduces the background signal and permits utilization
 of a small  aperture (1. 8 mm dia. ) just behind the entrance Brewster angle
 window. It should be noted that  the LNMP operates without a noticeable
 increase in background at low ambient light levels.  This desirable feature
 has been achieved with the combination of lens and  baffles (particularly
 the two baffles just  behind the entrance Brewster angle-window).  The
 features described above which function  primarily to reduce background
 cannot be considered an invariable feature of the LNMP.  We have opera-
 ted satisfactorily  a  breadboard model in  our  laboratory  without the  lens
 and baffles of Figure 6.
        The design of the chamber  (Figures 5 and 6) for  the LNMP is a
 practical implementation of a working design and should not necessarily
 be considered optimal.  Most of the components within the  chamber have
been configured to minimize the  background signal.   The entrance and
 exit windows are fabricated of fused silica (low visible fluorescence)
and are carefully  polished to minimize scattering of laser  light.   In the
                                - 4 -

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arrangement of Figure 6, a unique feature of the LNMP is the specially
designed liquid filters.
        Filter section 2 (the center section) is filled -with an aqueous solution
of Na2  Cr2Oy . 2H2O (304 g/1).   This  solution strongly absorbs the scat-
tered 442 nm laser light  without any detectable fluorescence and,  in ad-
dition,  strongly absorbs  the scattered Raman light due to O_, N_ and
water vapor.  When using 442 nm excitation,  the water vapor line
(furthest towards the red) occurs at 526 nm.  The transmission charac-
teristics of a Na~ Cr_ O_ filter are shown in Figure 7 for a 304 g/1 solu-
tion of  1 cm path length which is  identical to the concentration and path
length of filter section 2  (Figure  6). From Figure 7, it is evident that
the 304 g/1 Na_ Cr_ O_ solution of 1 cm path begins transmitting at 550 nm
and  sharply rises to  90% transmission at 600 nm.  The absorption coeffi-
cient of this filter at 442 nm is,  k = 1034 cm   .  The absorption coefficient
is defined  by:  I  = I  e     (Beer's Law).  A Corning  glass filter No. CS
3-66 (low wavelength cutoff at 555 nm) was  placed in front of the PMT
to provide additional attenuation at the Raman line of water  vapor  (526 nm).
        In the  two filter sections (1, Figure 6), a solution of 600 g/1 CoSO.
and a 600 g/1 NiSO. solution was  used.  This was found to result in fewer
background counts than that obtained with a 312 g/1 solution of Na? Cr~ O_
       The cells containing the solutions were fabricated of fused silica.
Fused silica is preferable to pyrex glass because its broad-band red
fluorescence is much weaker than that of the pyrex glass.  The  inner
diameter of the filter cells was approximately 2  cm.
       Six baffles (shown in Figure 6) were used to reduce scattered laser
light and, thereby, resulted in lowering the background signal.  The two
baffles just behind the front Brewster window provided the greatest  re-
duction in the background.
                                 -  5 -

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        The final element in the chamber is the beam absorber,  designed
to absorb the laser beam with a minimal increase in background.  This
is accomplished with a liquid filter cell with a fused silica Brewster angle
entrance window filled with the 304 g/1 sodium dichromate solution.  It
is obvious that improved sensitivity could be obtained by utilizing a mirror
to reflect the laser light back along the same path and increasing the path
length over which the  fluorescence is observed.
        A  simple means of  increasing the  sensitivity was employed and
consisted of a cylindrical front surface aluminized mirror on a fused
silica  substrate placed around the rear of the center section filter to
reflect fluorescence light into the PMT.   This resulted in a signal increase
by a factor  of about 1. 5 and a small increase in the background
        3.    Electronics Subsystem
        The fluorescence is monitored by a cooled PMT  (EMI 9659 QAM)
followed by a wide band amplifier and a high speed electronic counter
to count the electrical pulses produced by light impinging on the photocathode.
An RF shielded PMT refrigerator chamber minimizes interference from
external sources of RF  radiation.  The PMT is operated in a grounded
cathode configuration with  the cathode attached to the RF shield.  The PMT
is cooled  to -25 C to reduce the number of dark current  pulses.  Under
separate cover, a full set  of the manufacturer's instruction booklets and
detailed circuit diagrams of each component has  been delivered to EPA
personnel together with the LNMP at Research Triangle  Park,  North
Carolina.  A detailed  circuit diagram of an integrator which is incorporated
into the LNMP designed by the Aerospace Corporation is part of the detailed
description of the unit.  In  addition, a very detailed set  of  instructions for
operation and adjustment of the instrument has been included with the opera-
ting manuals.  Thus in this report only a brief description of the LNMP
electronics  will be included.
                               - 6 -

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        The output pulses of the cooled PMT are fed to a wide band, high
 gain amplifier/discriminator (SSR Model #  1120).  The output of this
 amplifier consists of pulses of standard 25  nsec width and -1. 5V ampli-
 tude and are fed to a 100 MHz counter (Newport Labs, Model # 700).
 A clock and printer, together with the required storage circuit are
 provided to secure several types of operation.  In the repetitive timed
 mode of operation,  the counter records the number of counts in a pre-
 set interval (10 sec, 100 sec,  and etc. ), determined by the internal
 time base of the counter.  At the end of this period,  this  information  is
 transferred to a storage circuit.  The counter is automatically reset
 and begins recording counts for the next consecutive period.  The  coun-
 ter dead time during the resetting is about 0. 1 sec.  The number of
 counts  recorded is transferred to the dials  of the print unit together
 with the date and time and is printed out on a 2-1/4 inch wide strip of
 paper.
        The alternative mode of operation is provided by means of  an
 electronic  integrator.  In the integrator mode, after a preset laser
 energy is measured (for example, 10 mW for 100 sec results in 1  J total
 output), the number of  counts accumulated by the counter is transferred
 to the  storage circuit then to the  printer wheels of  the printer unit and
 is printed out on paper tape together with the date and time.  The advan-
 tage of the integrator mode of  operation is that the signal counts recorded
 during  the  interval depend only on the total  preset laser energy delivered
 to the  sample and not on the instantaneous power levels during the interval.
 Thus,  the results are independent of the power variations during the inter-
 vals-
        For continuous monitoring,  it is desirable that the data be  pro-
 cessed by a computer.  With simple modifications,  the LNMP could be
 provided with an on-line computer which would process the data and pro-
vide a  read-out of the concentration of NO_  in the ambient atmosphere.
                                         c*
                                - 7 -

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        Photon counting is among the most sensitive methods of detection
of low light level signals.  The heart of the system is the PMT which
provides approximately noiseless amplification of the photon signal.
A photon that produces a photo-electron,  is amplified and produces a
pulse of current which signals this event.  The current pulse exhibits,
considerable variability in terms of amplitude, width,  etc. , but this
does not prevent an unambigous recording of the release of a photo-
electron.
        The difficulty with the PMT resides in  the existence of other  pro-
cesses which result in the  release of an electron producing a current
pulse indistinguishable from that generated by photons.  Among these
effects are dark current or thermionic emission from  the photocathode,
UV radiation produced by cosmic-ray induced  Cerenkov pulses, other
                                                           345
effects of cosmic rays and unidentified sources of radiation.  '  '   A
selected EMI 9659  cooled to -25  C operates with about 45 c/sec (with
the shutter closed).
        After adjustment of the  PMT high voltage to secure operation on
the ''plateau", it -was hoped that the fluctuations in the  counts could be
described by Poisson  statistics.  Tests with a selected low dark current
tube are summarized  in Table I.  A minimum  detectable concentration
is defined to be that for •which the variation in  the number of signal
counts just equals the number of  signal counts, namely,  S/a  = 1, where
S is the signal due  to the NO- concentration, and O is one standard devia-
tion of the  signal counts. According to the results shown in Table I,
an operating detectability of 0.9 ppbv was obtained for  a 100 sec inte-
gration time.  Had the fluctuation exhibited a Poisson distribution,  a
detectability of 0.41 ppbv would have resulted.  Other  investigators have
also noted  departures from Poisson statistics  and have tried to  identify
           345
the causes.  '  '   Note that by  increasing the integration time the detec-
tability increases (as the square root of the integration time) and, in
principle,  very high sensitivites can be obtained by utilizing long integra-
tion periods.   The  presence of background counts at low NO~ levels
                                - 8  -

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 reduces the sensitivity.  For example, if the background counts had been
 eliminated,  the detectability of the LNMP would be 0. 09 ppbv a ten fold
 improvement over the value listed (0.9) for a  100  sec integration time.
 IV.     CALIBRATION AND SYSTEM PERFORMANCE
        In this section, -we will summarize many tests carried out to
 delineate the performance of the L/NMP.   All tests specified in the contract
 have been  satisfactorily  completed.  The detectability of the LNMP is
 better by more than a factor of ten than the contract specification of
 10 _+ 5 ppbv (parts per billion per unit volume).
        In prior •work  on a similar system,  no interferences with NO_
                                                                   L*
 measurements had been  detected as a result of the presence of other gases.
 In the present contract,  tests were made for possible interference resulting
 from water vapor up to the  saturation value.  A vapor saturated air sample
 was prepared and directed through the chamber and did not result in a sig-
 nificant increase in the background counts.  Therefore, we concluded that
 the presence of water vapor up to the  saturation point did not show a de-
 tectable signal.  The fact that water-vapor does not interfere with NO_
                                                    12
 measurements was already known from earlier tests. '
        The tests involving calibration and linearity of the LNMP specified
 in the contract have been performed and have shown the LNMP calibrations
 to be highly reproducible.  Within experimental error the response of the
 LNMP was  shown to be linear over the range of 1 pphmv to 1 ppmv.
        One of the tasks specified in the contract was the determination
 of the calibration  constant of the  unit for  10 consecutive days.   The pur-
 pose of these tests was to demonstrate the  repeatability and reliability
of the LNMP.  A summary listing of the measurements performed on
 11 consecutive working days is shown  in Table II.  The uncertainty in
the calibration constant is due to the statistical fluctuation of the back-
ground and signal counts  and mostly due to the uncertainty of the known
NO2 sample (_+ 2. 5%).   However,  73%  of the values fall within 1 standard
deviation of the mean.  We consider the minimum  detectability  (D) of the
                                 - 9 -

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system to be  \/2 times the standard deviation (a) of the background counts
divided by the calibration constant(C), (D = 1.4
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 50 pphmv, the background levels begin to show a marked increase.  This
 is apparently due to adsorption of NO- on the -walls of the chamber and the
 connecting tubes.  The background can be reduced to a  low level by opening
 the chamber and cleaning.  In normal use where concentrations seldom
 go above  10 pphmv, the system was observed to operate for long intervals
 without any increase in the background counting rate.
 V.      AMBIENT AIR NO  LEVELS AT  LOCATION OF THE
        AEROSPACE CORPORATION
        After completion of the calibration and linearity tests,  the LNMP
 was  used to monitor the ambient air in the vicinity  of our building.  The
 general geographical location  is just southeast of Los Angeles  International
 Airport near the intersection of Aviation and El Segundo Boulevn Ms.  The
 air sample was drawn into the lab through PVC tubing with an orifice  15 ft.
 above the roof of our building; the overall length of the  piping to our cham-
 ber was approximately 40 ft.   A flow rate of 4. 5  liters/min.  was used and
 the air  sample was filtered to remove aerosols.
        The data reduction -was accomplished with our CDC 7600 computer
 and the computer generated curves  showing the ambient levels of NO9 are
 shown in Figures 11-13. A noteworthy feature  is the  low levels of NO-
 during a rainy period (Figure  11) and  the higher levels with the return of
 the usual Los Angeles climatic pattern (Figures  12 and 13).  The 28th and
 29th of  March were cloudy days.  On the 30th of March, it rained and on
 this day (Figure 11) the lowest concentrations of NO., were observed.
 CONCLUSIONS
        The  construction and operation of the Laser NO_ Monitor Prototype,
                                                     LJ
 developed for>the EPA under contract No. 68-02-1255, are described in
 this report.  Several new features not present in earlier embodiments of
 the laser-induced NO_ fluorescence monitor,  were  incorporated.  A He-
                    L*
 Cd laser was used for excitation. This provided an economical and com-
pact excitation source.   A new type  of low fluorescence solution filter .
                                - 11 -

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•was developed which resulted in greatly improved sensitivity.  The LNMP
was found to operate in a highly reproducible manner (Table II) and
possessed a detectability almost a factor of 10 greater than specified
by the contract.  Saturated water vapor levels  did not result in any
detectable interference.  The response of the instrument with respect
to concentration of NO? was found to be strictly linear over the range
tested,   1.0 to 100  pphmv.  Measurements of  the ambient NO_ levels
in the atmosphere in the vicinity of our laboratory building showed a
highly satisfactory performance of the instrument.
                                  - 12 -

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                              REFERENCES
1.     J. A. Gelbwachs, M.  Birnbaum,  A. W.  Tucker and C. L. Fincher,
       "Fluorescence Determination of Atmospheric NO_, " Optoelectronics,
       Vol. 4, pp. 155-160 (1972).

2.     A. W. Tucker, A.  Peterson and M.  Birnbaum,  "Fluorescence
       Determination of Atmospheric NO and NO_, "  Appl. Optics,
       Vol. 12, pp. 2036-2038 (September 1973).

3.     M.  Gasden, "Some Statistical Properties of Pulses from Photo-
       multipliers, "  Appl. Optics,  Vol. 4, pp. 1446-1452 (November
       1965).

4.     J. P. Rodman and H.  J. Smith, "Tests of Photomultipliers for
       Astronomical Pulse-Counting Applications,"  Appl. Optics,
       Vol. 2, pp. 181-185 (February 1963).

5.     R. G.  Tull, "A Comparison of Photon Counting and Current
       Measuring Techniques  in Spectrophotometry  of Faint Sources,"
       Appl. Optics,  Vol. 7,  pp.  2023-2029 (October 1968).

6.     D. C.  Baird,  "Experimentation: An Introduction to Measurement
       Theory and Experiment Design,"  (Prentice-Hall,  Inc., Englewood
       Cliffs, New Jersey, 1962).
                                   - 13 -

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                       ABBREVIATIONS
Laser NO7  Monitor Prototype
parts per billion per unit volume
parts per hundred million per unit volume
parts per million per unit volume
photomultiplier tube
LNMP







ppbv







pphmv







ppmv







PMT
                             - 14 -

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                            LIST OF TABLES
I.   Performance of Prototype.
II.  Instrument Sensitivity -
    Consecutive Daily Calibrations
    (11 days).
                                   - 15 -

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                         TABLE I

                PERFORMANCE OF PROTOTYPE
                     lOmW at 441. 6nm
S/<7 = 1
Poisson Statistics
Measured Values
Min. Det. Concentration (ppbv) , D
Integration Time
10 sec 100 sec
1. 3
2.9
0. 4
0.9
S is the number of counts due to the NO_ concentration.
a is the standard deviation in S.
D   = ale
 m
C = calibration constant
                         - 16 -

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              TABLE II

         INSTRUMENT SENSITIVITY

CONSECUTIVE DAILY CALIBRATIONS (11 DAYS)
            SIGNAL COUNTS
MINIMUM DETECTABLE
CONCENTRATION 80 SEC
INTEGRATION TIME
Day in March 1974
13
14
15
16
18
19
20
20
21
22
23
for one pphmv
8706 + 211
8430 + 204
8732 + 215
9274 + 229
8720 + 213
8379 + 216
8289 + 205
8870 + 230
8780 + 218
8835 ± 220
8846 + 216
(pphmv)
0.059
0. 065
0.033
0. 044
0. 066
0. 090
0.074
0.057
0. 061
0.059
0.056
         INSTRUMENT CAPABILITY

             1 pphmv _+ 0. 06 pphmv

         CONTRACT SPECIFICATION

             1 pphmv _f 0. 50 pphmv
                - 17  -

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                          LIST OF FIGURES
 1.     Laser NO? Monitor Prototype.

 2.     Laser NO_ Monitor Prototype - front view with cover panels removed.
                LJ

 3.     Laser NO- Monitor Prototype - rear view with rear access doors
       open.

 4.     LNMP electronics and data recording subsystem.

 5.     LNMP optical subsystem.

 6.     Block diagram of optical subsystem.

 7.     Spectral transmission of the Na?  Cr_ O_ . 2H?O  aqueous solution
       (304 g/1, 1 cm path length).

 8.     Linearity test for NO,, monitor in the 1  to 10 pphmv range for
       filtered and unfilterea samples.

 9.     Linearity test for NO- monitor in the 10 to 100 pphmv range for
       filtered and unfilterea samples.

10.     Optical  power linearity test for NO2 monitor.

11.     Atmospheric NO? concentrations,  El Segundo,  California
       March 28-30, 1974.

12.     Atmospheric NO- concentrations,  El Segundo,  California
       April 1-3,  1974.

13.     Atmospheric NO? concentrations,  El Segundo,  California
       April 4  and 5, 1974.
                                 -  18  -

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Figure 2,  Page 20

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Figure 3,  Page 21

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                                              Figure 11, Page 29

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