Environmental Protection Agency ALASKA WATER LABORATORY QUARTERLY RESEARCH REPORT October 1 -December 31, ALASKA WATER LABORATORY Fairbanks, Alaska 99701 ------- ALASKA WATER LABORATORY QUARTERLY RESEARCH REPORT OCTOBER 1 - DECEMBER 31, 1971 ------- ALASKA VILLAGE DEMONSTRATION PROJECT PROJECT: 16100 ZNM Objectives: To plan and construct, in one or more Alaskan villages, projects to provide a central safe v/ater supply and waste disposal facility in conjunc- tion with community laundry and bathing facilities and a community health education program to help assure continued beneficial use of the projects. Work Accomplished Second Quarter FY 1972: Two preliminary designs and business proposals for construction and installation of facilities at Emmonak and Uainwright were subjected to detailed review. Quotations for construction far exceeded funding limita- tions in each case. After prolonged communications with Washington, D.C. Headquarters, with designers, and with village councils, agreement was reached to reduce the scope of work to be accomplished. Major savings will be achieved through: 1. Reduction of floor space by approximately half. 2. Prefabrication to the point where equipment will be factory-installed in building modules sized to permit easy handling and shipment by Hercules air- craft. 3. Reduction to a minimum of skilled labor needed at s i te. 4. Possibility of utilizing BIA Plant Design and Construction building crews for installation. Construction contracts for both projects are now in the final stages of formal approval. Delivery of facility modules is expected in June and provision of full services should be possible by September 1972. The effects of this economizing exercise on the demonstration value of the project are several. The central facilities have been reduced from a scale considered optimal for Emmonak and Wainwright to a level at which basic services will still be provided, but conveniences to the operator and user have been trimmed to bare minimums. Because consumer acceptance and beneficial functioning of systems-type innovations inevitably depend upon their convenience value as well as upon technical merit, the potential of the demonstration facilities as viable alternatives to conventional water and sewer services in rural Alaska has been impaired. On the other hand, availability of relatively complete, inexpensive and easily trans- portable service modules could well accelerate the pace at which the bulk of small villages can be provided with skeletal services as an interim solution to their water and waste problems. Energy requirements, especially in the waste treatment section have continued to be a major concern. As a promising alternative to incineration, ------- application of a wet oxidation process has come under consideration. The so-called Zimmerman Process is used in Japan to treat night soil from homes and railways. At this point in time, however, equipment of the right size for use in small communities has not been built. The possibility, however, of incorporating such a unit in the Wainwright plant still remains open. Along these same lines, interest has been generated in the Bureau of Mines to find means to enable Wainwright to exploit known coal deposits in the vicinity of the village. Availability of coal could cut fuel costs in half. The plant, therefore, is being built to permit easy conversion from oil to coal once it is mined. As part of its contribution to the utilities system at Wainwright, the Indian Health Service has arranged for installation of the piling which will support the water storage tank at that location. Work Plan Thrid Quarter FY 1972: 1. Work closely with contractors to promote timely redesign and construction. Monitor expenditures to guard against overruns. 2. Refine vehicular delivery and pickup system. 3. Provide additional operator training. 4. Resume liaison with State and Federal Agencies involved in and contributing to the project. ------- COLD CLIMATE RESEARCH PROJECT 16100 ZNB - COLD REGIONS EXTENDED AERATION DESIGN CRITERIA Objectives: To develop empirical cold regions extended aeration design criteria from the operation of two adjacent parallel exposed units (aeration tanks). One unit has an enclosed conventional (horizontal flow) clarifier; the other, an enclosed upflow clarifier with tubes. Work Completed Second Quarter FY 1972: Considerable pumping and flow control problems were experienced with the Eielson A.F.B. comminuted sewage. It was decided to use primary effluent for feed. The feed pumps were moved to the end of the Eielson A.F.B. primary plant and there has been no major clogging problem since. Data has been collected while operating at approximately 10°C, 18 hour detention with 0.5 gpm/ft overflow rate. Initially the cooling pond did not reduce the feed temperature as expected. The evaporative cooling capacity (heat absorbing) of air from 0 to 30°F is only one third that from 60 to 90°F wet bulb air tempera- tures. The cooling pond has been converted to a spray pond and the feed temperatures are now down to 4°C or less. The upflow (with tubes) clarifier has not been performing as well as the horizontal clarifier. Floatable accumulation has been a problem on both clarifiers, but has been easier to handle on the horizontal clarifier due to easier surface access. Floatables have been pumped from atop the upflow clarifier to the sludge disposal lagoon. Work Plan Third Quarter FY 1972: To collect performance data for comparing the horizontal flow vs. the upflow (with tube settler) clarifiers under low temperature operation. PROJECT 16100 ZNE - COLD REGIONS AERATED LAGOON DESIGN CRITERIA Objectives: To establish design criteria for the aerated lagoon waste treatment process in Alaska, and to evaluate parameters and contribution to effluent quality, power and maintenance costs, and reliability. To establish the feasibility of using open type (non-clogging) diffusers in an aerated lagoon and obtain design information. ------- To determine the comparative effects of open type diffusers and perforated tubing diffusers on aerated lagoon process efficiencies. Work Completed Second Quarter FY 1972: Monitoring of the pilot aerated lagoon at Eielson A.F.B. and the Ft. Greely aerated lagoon continued through the quarter. The two cells of the Ft. Greely lagoon modified to include non-clog aerators have compared favorably with the perforated lead keel tubing aerators retained in the remaining two cells. This is in reference to overall treatment effi- ciency as well as operation and maintenance requirements. Comments from operating personnel at Ft. Greely indicate they have been favorably impressed with the lower maintenance requirements of the non-clog dif- fusers to date. Analysis of the dye study (for short circuiting) performed last quarter indicates both sides of the Ft. Greely lagoon are complete mix basins with little evidence of plug flow. They are not complete mix in the sense that all suspended solids are kept in circulation, however, as there are large deposits near the influent lines. Complete analysis of the data is not possible because of interferences encountered (bio adsorption, etc., which was expected). One phenomenon observed, however, was the apparent flow of influent around the perforated lead keel tubing bubble pattern and along the edge of the lagoon for a long distance before mixing into the bubble pattern. The influent scoop sampler has been removed and replaced with a laboratory fabricated sampler which is more effective. One limited oxygen transfer test has been performed and another will be performed during the third quarter. The ratio of flow to each side of the Ft. Greely lagoon has been determined with approximately 60% going to the non-clog diffuser side. The influent flow meter has proven unreliable. Flows will be estimated from the wet well pumping times. Work Plan Third Quarter FY 1972: At Eielson A.F.B. - Continue monitoring the pilot lagoon. At Ft. Greely: 1. Continue sampling on a biweekly basis. 2. Perform oxygen transfer efficiency test. 3. Prepare aerated lagoon design paper for technology transfer seminar. ------- PROJECT 16100 ZRD - COLD REGIONS SLUDGE DIGESTION AND DEWATERING Objectives: To demonstrate the feasibility of using shallow lagoons and the cold environment as a means of concentrating and dewatering waste activated sludges and to gather enough information from the process to determine practicality. Work Completed Second Quarter FY 1972: There has not been enough solids accumulated in the extended aeration units to fully utilize (test) this sludge disposal method. The digestion lagoon is less than half full. The MLSS concentration in this lagoon has dropped from over 2,000 to less than 1,000 ppm due to capture in the growing surface ice. Work Plan Third Quarter: Mixed liquor (from the extended aeration pilot units) will be wasted into the lagoon as available. Ice cores will be taken and evaluated for SS capture during feed and possible elutrition during periods of ice formation without feed. PROJECT 16100 ZNH - SIGNIFICANCE OF BOD AS AN ALASKA WATER QUALITY PARAMETER Objective: To define the effects of arctic and subarctic conditions on the BOD progression by investigating the combined effects of temperature, turbulence and river water dilution on BOD reaction rates and the BOD test. Work Completed Second Quarter FY 1972: Work continued on establishing laboratory techniques throughout the quarter. These have been essentially worked out and the actual experiment will begin during the third quarter. The experiment is approximately one month behind schedule due to unexpected delays encountered in establishing laboratory techniques. Work Plan Third Quarter: Begin the actual experiment. PROJECT 16100 ZQD - LOW TEMPERATURE DISINFECTION Objectives: To develop laboratory and field technics for studying effectiveness of disinfectants. ------- To determine the effectiveness of chlorine disinfection at low temperatures on primary and secondary sewage treatment plant effluents. Work Completed Second Quarter FY 1972: Several weeks were spent "debugging" the system. The worst problem was maintaining the samples at 0°C throughout the 4 hours of contact time. Other problems included getting an adequate composite sample of the effluent and lowering the sample temperature to 0°C before starting the timed disinfection study. The temperature problems were overcome by using a large refrigerated water bath. The effluent was initially placed in a 6 gallon polyethylene carboy, cooled to 0°C in the water bath set at -5°C while stirring with a propeller stirrer. Samples for the timed disinfection studies were withdrawn from the 6 gallon carboy, placed in 4 liter glass carboys and stirred with submersible magnetic stirrers in the water bath at 0°C. The problems of determining the amount of Cl2 necessary to give a 1 rng/1 residual at 1 hour and the dilutions to use to determine total and fecal coliforms were overcome by what can be termed a pre-experiment run. The effluent was brought to the Laboratory on Wednesday morning and immediately cooled to 0°C. An aliquote was used to determine the number of total and fecal coliforms present. Various concentrations of chlorine were added to 1 liter samples and checked for chlorine concentration after 1 hour contact time. The sample having approximately 1 mg/1 residual after the hour was examined for total and fecal coliforms. The effluent was held at 0°C until Thursday morning when the coliforms were counted. The sample volumes for bacterial examination were determined from these counts. Three samples for disinfection were set up with the chlorine concentration which had given 1 mg/1 as the middle concentration and bracketed 0.5 mg/1 on either side. Controls were unchlorinated samples at 0 and 25°C and a chlorinated sample at 25°C. The changes which may have taken place during the 24 hour holding time at 0°C did not appear to alter the chlorine demand or the coliform count. Three disinfection runs on secondary effluents were done over a three week period with this method of sample handling. The temperature during the runs was between 0 and 1°C, and the total coliforms ranged from 1-8-20 x 10^/100 ml while the fecal coliforms ranged from 0.78- 2.5 x 10b/100 ml. Since the ammonia nitrogen and suspended solids were essentially the same, the efficiency of kill during the first hour of contact time appeared to be directly related to the initial number of coliforms present. These data suggest that the standard of 1000 total and 200 fecal coliforms can not reliably be met with a 1 mg/1 C12 residual and 1 hour contact time when the effluent temperature is near 0°C. Work Plan Third Quarter FY 1972: Disinfection studies similar to those just described will be run at 5°C and possibly at 10°C to determine at what temperature a reliable kill can be obtained with a 1 mg/1 Cl2 residual and 1 hour contact time. Some ------- work will be carried out to determine if incremental addition of C12 will have any positive effect on the efficiency of kill at 0°C. Since no adequate operating contact chamber is available, we plan to design and build a 4 compartment chamber which will hold 60-65 liters of ef- fluent so that we can study the effect of Clg in a flow-through system which approaches operational systems in design. PROJECT 16100 ZND - LOW TEMPERATURE MICROBIOLOGICAL ACTIVITY AND P.O. DEPLETION Objectives: 1. Complete the rate study of the stationary vs. dynamic closed system and effect of velocity of the water on rate of D.O. depletion at 0° and 5°C. 2. Survey gross nutritional requirements of selected pure cultures of indigenous river bacteria which are capable of rapid D.O. depletion at 0° and 5°C with protein as the nutrient. 3. Determine the effect of nutrients required for the pure cultures on the rate of D.O. depletion by the natural mixed population of bacteria in unpolluted river water. Work Completed Second Quarter FY 1972: Because of the time required to perform the work on the Low Tempera- ture Disinfection study, progress on this project has been very limited. The pure cultures for the nutritional studies have been maintained as necessary to ensure their viability. Glucose fermentation by the pure cultures was examined and the results in Phenol Red Broth were somewhat inconclusive because some of the cultures are weak acid producers and some reduce the phenol red rather than produce acid. Some effort has been expended to determine if another acid/base indicator would perhaps provide more definitive results. Because of the limited time available for this study, microtechniques are being examined to determine if they can be adapted for use in the nutrition work in order to reduce the amount of time required to complete this portion of the study. The monthly enumeration of the indigenous bacteria in unpolluted river water is continuing at 0°, 20° and 35°C. The 35°C count is always very low (<100/ml.). As winter progresses, the 20° and 0°C counts have continued to decrease with a more dramatic change found in the 20°C count. Work Plan Third Quarter FY 1972: The nutritional studies and the monthly counts will continue. ------- PROJECT 16100 XCN - WINTER P.O. CONDITIONS IN ALASKAN" WATERS Objectives: 1. Examine the effects of low dissolved oxygen concentration on aquatic organisms. 2. Examine some of the causes of winter low dissolved oxygen phenom- enon that has been documented in Alaskan streams. 3. Continue on a limited time and money available basis the survey of winter D.O. conditions in Alaskan waters. More emphasis will be placed on lakes and pond waters. Work Completed Second Quarter FY 1972: A manuscript that discusses Winter D.O. in Alaskan rivers and streams has been distributed for informal review. This paper contains D.O. data and limited other water chemistry collected over a period of years. Dis- solved oxygen, conductivity, alkalinity and pH data have been tabulated from a number of Alaskan rivers. Trends and patterns that appear during the winter are discussed with an orientation toward management problems. An informative trip was taken to the National Water Quality Laboratory at Duluth to confer with NWQL staff about the equipment utilized in their studies. Particular attention was paid to the degassifier although numerous instrument control packages were examined. Since this trip, instrument limitations have been discussed with AWL staff. Subsequently, specification requests have been sent to commercial firms. Concurrently, a system is being designed that will fit physical facility needs and examine the effects of low D.O. at low temperatures on cold climate organisms. Data have been collected from three of the four winter field stations. Information collected includes quantitative and qualitative macrobenthic samples, and several physical-chemical parameters including dissolved oxygen, pH, conductivity and alkalinity. Analysis of the biological samples is underway. Those chemical samples that were brought to the laboratory for analysis are being analyzed. At this time, it would be premature to make any statements about the results. The last series of experiments for validation of the nitrogen D.O. sampling technique were conducted. Statistical analyses of the data were made to determine reliability and significance. Preliminary conclusions have been made that the nitrogen sampling technique is reliable under extreme cold conditions. Results of the validation experiments and tests of significance are being placed in draft form for informal review. Work Plan Third Quarter FY 1972: Winter D.O. manuscript corrections and redraft will begin once the draft is returned from informal review. Tables, figures, and photographs will be drafted and edited for final publication. Copies of semifinal draft will be distributed for formal review prior to publication. ------- Once the specification requests have been received, design of the instrumentation system will continue. Construction or purchase of the system is planned. The choice depends upon the cost, specifications, and bids received from the commercial enterprises. Plans include collecting biological, physical, and chemical infor- mation from the four winter stations during each month of the winter season. PROJECT 16100 ZOB - MANAGEMENT OF GRAVEL REMOVAL PRACTICES ON THE NORTH SLOPE TO ENHANCE WATER QUALITY Objectives: The overall objective is to ascertain the effects of stream bed dis^ turbance on stream hydraulics and sediment loads, how these sediments influence water quality and short and long-range effects of sediments on aquatic life. Objectives for FY 1972 will include becoming familiar with methods to accomplish the study, select reaches of representative streams likely to be disturbed and collect and analyze samples of water, gravels, and invertebrate fauna of several streams. Based on these preliminary findings, an additional objective will be to define and plan in detail, a program of research to obtain definitive answers to questions relating to realistic management of the aquatic resources as they are affected by construction activities. Work Completed Second Quarter FY 1972: No field work was done on this project; field work planned for October was delayed until cold weather arrived which prevented making the proposed trip to Salcha River. A write-up of observations made during the Wickersham Dome fire was completed; this brief paper describes some sedi- mentation phenomena associated with the fire which is part of the overall sedimentation project. Discussions with members of the Forest Service at the U of A resulted in the decision to prepare a preliminary set of guidelines dealing with logging roads in Interior Alaska. This effort will be jointly accomplished by BLM, State Land and Forest officials, Forest Service, and AWL, as part of the broad sedimentation project. Work Plan Third Quarter FY 1972: Discuss biological areas of needed research dealing with sedimentation at Con/all is with EPA personnel and members of OSU staff who have experience in the effects of sediments on aquatic life. Prepare a work plan for FY 1963 based on suggested critical needs and coordinate future plans and activities with others studying sedimentation effects such as U.S.G.S. or State Fish and Game. ------- 10 PROJECT 16100 ZQL - ECOLOGY OF TUNDRA LAKES Objectives: The Tundra Lakes project is part of the International Biological Program. This comprehensive program is intended to study various biomes to better understand and manage the areas. Five biomes, grassland, desert, deciduous, coniferous and tundra are being investigated. The IPB Tundra Biome intensive study site, located at Point Barrow, has three terrestrial subprograms and one aquatic subprogram. In each subprogram numerous studies are being conducted by various agencies and universities. In the aquatic subprogram these studies include: physical-chemical characteristics of ponds; microbiological metabolism in sediment; zooplankton population dynamics. With these and other studies underway, it is the objective of the AWL Tundra Lake study to develop a project that fills an existing need in the subprogram and is interrelated with existing studies. Work Completed Second Quarter FY 1972: This project was inactive during this quarter. Work Plan Third Quarter FY 1972: Work during this quarter will also be limited. Efforts will be made to coordinate and schedule the field season to coincide with the field work of other International Biological Program investigators studying tundra lakes and ponds. PROJECT 16100 ZOY - BASELINE STUDY OF A NATURAL SUBARCTIC WATERSHED Objectives: The overall objective is to obtain sufficient data on all aspects of the aquatic ecosystems in the Poker-Caribou Creek watershed over a long enough time interval to enable development of a watershed model as a reliable tool to predict dynamic behavior of subarctic watersheds when defined perturbations are imposed on them. To attain this broad objective the immediate objective is to identify, catalog, and evaluate the significance of each segment of the entire biota for each individual stream draining a subwatershed prior to disturbance; and to measure chemical and physical parameters under a variety of environ- mental conditions over a sufficient time span to evaluate interrelation- ships of environmental factors and the stream biota prior to disturbance. ------- 11 Work Completed Second Quarter FY 197Z: All scheduled field work for this project was completed the previous quarter. The only activity this quarter was completion of all lab analyses as outlined in the work plan and tabulating all field data. Tentative interpretation of field data suggests that subtle differences in field measured parameters persisted throughout the summer when comparing Poker with Caribou Creek. After some discussion, it was decided to have a geologic map and a four-inch-to-the-mile topographic map made for the entire watershed. Two groups at the University of Alaska were contacted who are interested in doing this work on a grant; no firm plans have been made as of this date. PROJECT 16050 XCU - FATE AND EFFECT OF OIL SPILLS ON ALASKAN STREAMS Objectives: 1. To characterize the nature and extent of oil as it is transported over and through organic (soil and vegetation) and inorganic layers after being applied to an Interior Alaskan watershed. 2. To demonstrate the influence of such things as runoff water and permafrost on transport of oil. 3. To determine changes in character of crude oil spilled on cold streams (0-5°C). Work Completed Second Quarter FY 1972: Most of the laboratory equipment involved in hydrocarbon analysis was put on line as a general procedure for the extraction and identification of crude oil fractions in soils and water was adapted and run in the lab. Known amounts of certain selected hydrocarbons were administered to soils from the Glenn Creek area and extracted later with methyl alcohol and/or pentane. Results are still being analyzed, but indications are that separa- tion of the applied hydrocarbon from naturally occurring interferences are good. Precision and accuracy of the techniques used will be determined upon further examination of the results. Despite some limitations due to equipment malfunctions, the laboratory now has a physical capability of accepting water or soil samples which are to be examined for hydrocarbons. As lab equipment is brought into proper order it is expected that analyses will become increasingly refined. Work Plan Third Quarter FY 1972: Due to delays caused by equipment failure and certain unforeseen procedural problems, the model stream study which was to be initiated last quarter will begin this quarter. ------- 12 Other work may include sampling near accidental oil spills on natural water bodies to follow the fate of oil. Extraction of soils will continue with the application and then extraction of actual crude oil. ------- 13 CONSOLIDATED LABORATORY SERVICES The number of analyses requested in the quarter, about 2000, is almost identical to that requested in Second Quarter FY 1971. Most of these analyses were conducted for the research branch. Final budget submittals were prepared based on the CLS analysis schedule for the first two quarters, and a projection for the remainder of FY 1972. Tne construction of the hazardous materials storage area is complete except for the installation of the roof membrane. Plans are to accept the building with the deficiency of the roof, which will be installed in the coming spring. Occupancy should be available in mid January, 1972. A series of short meetings were neld with officials of the Bureau of Land Management concerning their experimental power generation facility, which removes air pollutants with a condenser system. Because of the possibility of the effluent from the condenser containing residual fuel, sulfate, nitrogen compounds, acid wastes, etc., we have agreed to analyze the effluent for these and other constituents. Mrs. McFadden attended the training course "Analytical Quality Control" offered by EPA National Training Center, Cincinnati, Ohio. She has been appointed Laboratory Analytical Quality Control Officer. ------- 14 GRANTS AND CONTRACTS PROJECT 16100 EQM - "Baseline Water Quality Study of the Alaskan Arctic Estuarine Development," Institute of Marine Sciences, University of Alaska, Dr. Patrick J. Kinney. Limited field work continued in the second quarter. Many of the field investigators have returned to the laboratories where analyses continue on the collected samples. It is anticipated that these results will be made available during the third quarter. PROJECT 16100 FHQ - "Investigations on Possible Effects of Crude Oil on Aquatic Organisms of the Central Alaskan Fisheries," Department of Biological Sciences, University of Alaska, Dr. James E. Morrow. Work with Study of serus identification the sock-eye salmon and crude proteins with electrophoresis of the major protein bands is oil was completed in November. is continuing and approximate expected. PROJECT 16100 PAK - "Lime Disinfection of Sewage Bacteria at Low Temperature," Department of Microbiology, Colorado State University, Dr. S. M. Morrison. The effect of lime on total and fecal coliforms at pH 10.0, 10.5, 11.0, 11.5 and 12.0 was studied at 1.0°C in raw settled sewage. No treatment system showed 100% kill within the exposure intervals used. However, the death and survival rates were found to be dependent on pH and the duration of exposure. A 99% kill was obtained at pH 11.5 and 12.0 in 30 to 80 minutes. The difficulties experienced with inconsistent fecal coliform counts were overcome by mixing the sample with a vortex mixer just prior to membrane filtering. ------- 15 PUBLICATIONS AND PRESENTATIONS The publication "Environmental Guidelines for Road Construction in Alaska" was received from the printers and is now being distributed. Working Paper # 13, "Alaska Industry Experience in Arctic Sewage Treatment" was printed and is now available. Alaska Village Demonstration Project Advisor, Bert Puchtler, represented the Alaska Water Laboratory at a dinner meeting of the Fairbanks chapters of the ASCE and ASPE and gave a talk on AVDP concept and implementation. ------- |