xvEPA
                                United States
                                Environmental Protection
                                Agencv
                                Health Effects Research
                                Laboratory
                                Research Triangle Park NC 27711
                                Research and Development
                                EPA-600/S1-81-048  Aug. 1981
Project  Summary
                                Effects of  Post-Implantation
                                Exposure to Selected
                                Pesticides  on
                                Reproductivity  in  Rats
                                Fitzgerald Spencer
                                 The post-implantational effects of
                               dinoseb. PCB's (Aroclor 1254),
                               rotenone and zineb on reproductive
                               systems were examined using de-
                               cidualized pseudopregnant rat as a
                               model.  Uterine protein,  uterine
                               glycogen, uterine water, and ovarian
                               protein were  studied  in day-10 de-
                               cidualized pseudopregnant rats fed
                               the toxicants from days 6 through 9 of
                               pseudopregnancy. Dinoseb reduced
                               uterine protein and uterine glycogen in
                               rats fed 25 ppm and higher concentra-
                               tions. Uterine water and uterine
                               weight were reduced  at the highest
                               dosage of 750 ppm. Ovarian protein
                               was  diminished at 150 ppm  and
                               higher concentrations. PCB's lowered
                               uterine glycogen, but uterine protein
                               content was not reduced in a dose-
                               related manner. Ovarian protein con-
                               tent was diminished at 50 ppm and
                               higher concentrations. Uterine weight
                               and uterine water were not changed in
                               rats fed up to 1000 ppm of the PCB's.
                               Rotenone reduced uterine protein in
                               rats fed  200  ppm and higher con-
                               centrations. Uterine glycogen was
                               diminished at 10 ppm and higher
                               concentrations. In day-16  pregnant
                               rats fed rotenone (100, 200,400, and
                               600 ppms) from days 6-16 of preg-
                               nancy, placenta! protein, placenta!
                               glycogen, ovarian protein, and mater-
                               nal body weight were reduced. Addi-
                               tionally, these dosing  regimens re-
                                duced fetal survival rate. Fetal weight
                                of fetus delivered from rotenone-fed
                                dams was not affected. The decid-
                                ualized pseudopregnant uterine, and
                                placenta! functions and fetal survival
                                rate of rats were not affected by zineb
                                up to 2500 ppm.
                                  This Project Summary was devel-
                                oped by EPA's Health Effects Re-
                                search Laboratory, Research Triangle
                                Park. NC, to announce key findings of
                                the  research project that is fully
                                documented in a separate report of the
                                same title (see Project Report ordering
                                information at back).


                                Introduction
                                  The talidomide episode caused great
                                public concern and left no doubt on the
                                necessity of testing possible teratogenic
                                and fetotoxic effects of compounds that
                                may be  encountered by pregnant
                                women.
                                  Since the presence of many classes of
                                pesticides in  the environment offers
                                numerous opportunities for these
                                toxicants to enter the physiological
                                systems  of various species of animals
                                including man, there has  been an
                                increased interest in studying the
                                teratogenic effects of certain pesticides
                                on mammalian reproduction.
                                  Pesticides such as DDT have been
                                shown to reduce estradiol liter in blood
                                of ringdoves,  Streptopelia risoria. On

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the basis of experiments, the eggshell
thinning  action of chlorinated hydro-
carbons has been attributed  to direct
estrogenic action of DDT and its analogs
(RGB's).
  Because  pseudopregnant and preg-
nant rats have identical mechanisms for
controlling the onset and loss of uterine
sensitivity, the deciduomal response in
decidualized pseudopregnant  rats was
used to study  possible  reproductive
disruption as caused by various pesticides
in pregnant uteri.


Results and  Discussion
  Uterine weight and uterine water
content of decidualized pseudopregnant
rats exposed  to  dinoseb were only
reduced at the  highest dosage of 750
ppm.  In comparison  with the control,
uterine protein and  uterine  glycogen
contents were reduced at 25  ppm and
higher concentrations. Ovarian protein
content of the rats exposed to dinoseb
for  4 days was not affected up to 500
ppm. The difference in susceptibility to
dinoseb, between uterine protein con-
tent and ovarian protein content might
be attributed to a differential distribution
of dinoseb within the reproductive
system. In pregnant rats, exposed to
dinoseb for  10 days, ovarian protein
content was reduced at 200  ppm  and
higher concentrations.
  Ovarian protein of the decidualized
pseudopregnant (OCR PSPG) rats ex-
posed  to the  PCB, Aroclor 1254, was
reduced to 50  ppm  and  higher con-
centrations. Uterine protein content
was reduced at  25  ppm and higher
concentrations. This reduction was not
dose-dependent, and  the difference
was attributed  to abnormal control
value.  Uterine glycogen content of the
rats was reduced at 150 ppm and higher
concentrations. Uterine weight and
uterine water content, regardless of the
dose of the PCB's being tested, were not
affected.
  In OCR PSPG rats exposed to rotenone,
uterine weight was reduced at 100 ppm
and higher concentrations, but uterine
water content was not affected. Ovarian
protein content was not reduced up to
750 ppm. Uterine protein content was
reduced at 200 ppm, in comparison with
the control. Uterine  glycogen content
was decreased at 10 ppm and higher
concentrations. Placental protein con-
tent and placental glycogen content of
pregnant rats exposed to rotenone were
reduced. Ovarian protein content of rats
was diminished. Rotenone did not affect
the developing embryos. Fetal survivial
rate of the fetuses was decreased at 100
ppm and higher concentrations. Fetuses
delivered from dams exposed to rotenone
during the post-implantational stage of
pregnancy did not exhibit any reduction
in body weight. The levels  of zineb
tested in the intrauterine environment,
i.e. uterine protein,  uterine glycogen
and uterine water contents of the rats,
were  not affected. Ovarian protein in
both the pseudopregnant and pregnant
rats exposed to zineb for 4  days and 10
days,  respectively, was not affected.
Placental protein was not affected by
zineb. In comparison with the control,
placental  glycogen  content was  in-
creased. This  increase was  not dose-
dependent. Development of implantation
sites  in pregnant rats after  7 days of
exposure to zineb was not affected. The
fetal  survival  rate was not  affected.
Fetal  weight  was  reduced  but  this
decrease was  not  regarded  to be
compound-related since  it was not
dose-dependent.


Conclusions
  Dinoseb, PCB's (Aroclor 1254), and
rotenone are  uterotoxic, causing  dis-
ruption on the integrity of decidualized
pseudopregnant uterus. Since the
intrauterine environment is maintained
by sex hormones, namely, estrogen and
progesterone, anti-estrogenic, and anti-
progrestogenic properties of these
toxicants could be inferred.
  Rotenone interferes  with fetal de-
velopment. Because development of the
placenta  corresponds with that of the
fetus, any interference imposed on the
placenta by rotenone may be viewed as
essentially disruptive to the fetal
development  process. In  addition,
rotenone  is fetotoxic. The fetotoxic
potential of rotenone is designated by a
decrease in fetal survival rate.
  This  study shows that zineb has low
toxic effects  on the maternal repro-
ductive system and the fetus. This could
be attributed to the fact that zineb does
not accumulate to  a  large  extent  in
mammalian  tissues and could  be
metabolized readily.

Recommendation
  Further studies on the toxicological
effects of pesticides upon  the repro-
ductive system should be correlated
with the adrenal functions.
  Consideration should be given to
dietary intake of the animal during any
toxicological studies in reproduction.
  Fitzgerald Spencer is with the Department of Biological Sciences and Health
    Research, Southern University, Baton Rouge, LA 70813.
  August Curley is the EPA Project Officer (see below).
   The complete report,  entitled  "Effects  of Post-Implantation Exposure  to
    Selected Pesticides on  Reproductivity in Rats," (Order No. PB 81-213 209;
    Cost: $6.50, subject to  change) will be available only from:
          National Technical Information Service
          5285 Port Royal  Road
          Springfield, VA 22161
          Telephone: 703-487-4650
   The EPA Project  Officer can be contacted  at:
          Health Effects Research Laboratory
          U.S. Environmental Protection Agency
          Research Triangle Park. NC 27711
                                                                                 i US GOVERNMENT PRINTING OFFICE 1981 -757-012/7304

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Environmental Protection
Agency
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Information
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Fees Paid
Environmental
Protection
Agency
EPA 335
Official Business
Penalty for Private Use $300

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