United States
Environmental Protection
Agency
Health Effects Research
Laboratory
Research Triangle Park NC 27711
Research and Development
EPA-600/S1-83-017 Jan. 1984
&EPA Project Summary
Inhalation Teratology Studies of
Captan and Folpet in Mice
K. Diane Courtney, James E. Andrews, James T. Stevens, and Jackie D. Farmer
Timed pregnant CD-1 mice were
exposed to captan or folpet by the
intragastric, subcutaneous, or
inhalation route. A dose of 100
mg/kg/day of captan or folpet was
administered subcutaneously or
intragastrically from day 6 through 15
of gestation. The dose levels for the
inhalation route were averaged from
daily exposure levels determined by
monitoring the chambers. The
inhalation route provided daily average
concentrations approximating 491
mg/m3 for captan, and 624 mg/m3 for
folpet, four hr/day from the sixth to the
thirteenth day of gestation. The particle
size was less than 5//m. Approximately
10% maternal mortality was observed
with both captan and folpet by the
inhalation route, but no mortality was
seen by the other two routes. The only
fetal toxicity noted was a reduction in
fetal body weight in the group adminis-
tered captan subcutaneously. Neither
captan nor folpet was teratogenic in
CD-1 mice exposed by the subcutane-
ous, oral or inhalation routes.
This Project Summary was developed
by EPA's Health Effects Research Lab-
oratory. Research Triangle Park, NC, to
announce key findings of the research
project that is fully documented in a
separate report of the same title (see
Project Report ordering information at
back).
Introduction
Captan and folpet are fungicides with
structures generally similar to
thalidomide, which raises questions
about their teratogenic potential. Many
studies in the literature indicate that the
compounds are not teratogenic, whereas
some have found them to be teratogenic.
Thus, these fungicides were tested for
their teratogenic potential in mice by
using oral, subcutaneous, and inhalation
exposure routes.
Procedure
Pregnant mice with known gestational
days were treated with captan and folpet
daily from gestational days 6 through 15
at a dose level of 100 mg/kg either by
gavage or subcutaneous injection and
killed on day 17 An additional group of
mice was exposed to captan or folpet from
gestational days 6 through 13 and killed
on day 17.
Results and Discussion
Neither captan nor folpet was
teratogenic by oral, subcutaneous or
inhalation exposure routes. The exposure
levels of captan and folpet were maternally
lethal but did not affect the fetus.
Conclusion
Captan and folpet atdosesof 100 mg/kg
administered orally or subcutaneously
during organogenesis was not
teratogenic in the CD-1 mouse.
Inhalation exposure of CD-1 mice to cap-
tan or folpet at maternally lethal doses did
not produce fetal malformations.
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The EPA authors K. D. Courtney. J. E. Andrews, J. T. Stevens, and J. D. Farmer
are with the Health Effects Research Laboratory, Research Triangle Park. NC
27711.
Diane Courtney is the EPA Project Officer (see below).
The complete report, entitled "Inhalation Teratology Studies ofCaptan andFolpet
in Mice," (Order No. PB 84-128 099; Cost: $7.00, subject to change) will be
available only from:
National Technical Information Service
5285 Port Royal Road
Springfield, VA 22161
Telephone: 703-487-4650
The EPA Project Officer can be contacted at:
Health Effects Research Laboratory
U.S. Environmental Protection Agency
Research Triangle Park, NC 27711
*US GOVERNMENT PRINTING OfflCE 1984-759-015/7263
United States
Environmental Protection
Agency
Center for Environmental Research
Information
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