_ „ United States
  i Environmental Protection
  * Agency
Office of Research and
Development
Washington, DC 20460
EPA/600/R-01/021
March 2001
http://www.epa.gov
   Laboratory Study on the
   Oxidation of Arsenic III to
   Arsenic V

• \x
       As (III)
        As(V)

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                                               EPA/600/R-01/021
                                                   March 2001
Laboratory Study on the Oxidation
      of Arsenic III to Arsenic V
                       by

                  Ganesh Ghurye
                  Dennis Clifford
                University of Houston
              Houston, TX 77204-4791
            EPA Contract 8C-R311-NAEX
                   Project Officer

                   Thomas Sorg
       Water Supply and Water Resources Division
      National Risk Management Research Laboratory
                Cincinnati, OH 46268
      National Risk Management Research Laboratory
          Office of Research and Development
         U.S. Environmental Protection Agency
                Cincinnati, OH 45268
                                            Printed on Recycled Paper

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                                Disclaimer
The information in this document has been funded by the United States Environmental
Protection Agency (EPA) under Contract No. 8C-R311-NAEX to the Department of
Civil and Environmental Engineering at the University of Houston.  It has been subjected
to the Agency's peer and administrative reviews and has been approved for publication as
an EPA document. Mention of trade names or commercial products does not constitute
an endorsement or recommendation for use.

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                                  Foreword
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This publication has been  produced as part of the Laboratory's strategic long-term
research plan.  It is published and made available by the EPA's Office of Research and
Development to assist the user community and to link researchers with their clients.
                                 E. Timothy Oppelt, Director
                                 National Risk Management Research Laboratory
                                       in

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                                   Abstract
A one-year laboratory study was performed to determine the ability of seven oxidants to
oxidize As(III) to As(V).  These included chlorine, permanganate,  ozone,  chlorine
dioxide, monochloramine, a solid-phase oxidizing media, and 254 nm ultraviolet light.

Chlorine and permanganate rapidly oxidized As(III) to As(V) in the pH range of 6.3 to
8.3.  Dissolved manganese, dissolved iron, sulfide and TOC slowed the rate of oxidation
slightly, but essentially complete oxidation was obtained in less than one minute with
chlorine and permanganate under all conditions studied.

In the absence of interfering reductants, ozone  rapidly oxidized As(III).  Although,
dissolved manganese and dissolved iron had no significant effect on As(III) oxidation,
the presence of sulfide considerably slowed the oxidation reaction.  The presence of TOC
had a quenching effect on As(III) oxidation by ozone, producing incomplete oxidation at
the higher TOC concentration studied.

Only limited As(III)  oxidation was obtained using chlorine dioxide, which was probably
due to the presence of chlorine (as a by-product) in the chlorine dioxide stock solutions.
The reason for the ineffectiveness of chlorine dioxide was not studied.
Preformed monochloramine  was ineffective  for As(III) oxidation, whereas limited
oxidation was obtained when monochloramine was formed in-situ. This showed that the
injected chlorine probably reacted with As(III) before being quenched by ammonia to
form monochloramine.

Filox,  a  manganese  dioxide-based media, was effective for As(III) oxidation.  When
dissolved oxygen (DO) was not limiting, complete  oxidation was observed under all
conditions studied. However, when DO was reduced, incomplete oxidation was obtained
in the presence of interfering reductants. The adverse effect of interfering reductants was
completely eliminated by either (a) supplying enough DO or (b) increasing the contact
time.  In addition to oxidizing As(III),  the Filox media also removed  some arsenic by
adsorption,  which diminished greatly  as the media came  into  equilibrium  with  the
As(HI)-spiked synthetic water.

UV  light alone  (254  nm) was  not  very effective  for As(III) oxidation.  Significant
oxidation was observed only at very low flow rates representing 0.6 - 2.5% of the rated
capacities of the two UV sterilizer units tested.  However,  as reported in a patented
process,  complete oxidation by UV light was observed when the challenge water was
spiked with 1.0 mg/L sulfite.
                                        iv

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                                   Contents
Foreword                	 iii
Abstract                 	 iv
Figures                  	 viii
Tables                   	 ix
Abbreviations and Acronyms	 x
Acknowledgments        	 xi

1.  Introduction          	 1
   1.1     Background   	 1
           1.1.1  Previous Studies	 1
   1.2     Oxidants Evaluated	 2
   1.3  Oxidant Stoichiometry	 2
           1.3.1  Chlorine	 2
           1.3.2  Permanganate	 3
           1.3.3  Ozone  	 4
           1.3.4  Chlorine Dioxide	 4
           1.3.5  Monochloramine	 5
   1.4     Research Objectives	 5
2.  Materials and Methods	 6
   2.1     Synthetic Test Water Composition	 6
   2,2     Interfering Reductants	 6
   2.3     SpeciationMethod.....	 6
   2.4     Chemical Oxidation Experiments	 7
           2.4.1  Reactor Design	 7
           2.4.2  Testing Reactor Set-up	11
                 2.4.2.1   Arsenic(V) Retention.	11
                 2.4.2.2  Arsenic(III) Passage	11
                 2.4.2.3   System Dead Volume	11
                 2.4.2.4  Oxidant Mixing Efficiency	11
           2.4.3  Oxidation Conditions	12
                 2.4.3.1   pH of Operation	12
                 2.4.3.2  High- and Low-DO Experiments	12
                 2.4.3.3   Oxidation Experiments with TOC	13
                 2.4.3.4  Low Temperature (5 °C) Oxidation Experiments	13
   2.5     Solid-Phase Media (Filox) Experiments	13
           2.5.1  Pretreatment of Filox Media	13
           2.5.2  Preparation of Low- andHigh-DO Synthetic Water	14
           2.5.3  Procedure for Variable-EBCT and Variable pH Experiments	14
           2.5.4  Procedure to Stabilize Arsenic Removal by Filox Media	14
           2.5.5  Procedure for Low-DO Filox Experiments	15

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      2.6   UV Oxidation Experiments	15
            2.6.1    Unit 1: 0.5 gpmUV Sterilizer Unit	15
            2.6.2    Unit 2: 1.0 gpmUV Sterilizer Unit	16
      2.7           QA/QC	•.	 16
            2.7.1    Arsenic Standards	16
            2.7.2    Routine Arsenic Analysis	17
            2.7.3    Arsenic Calibration Curve	17
      2.8   Analytical Methods	18
3.     Results and Discussion	19
      3.1     Reactor Set-up Test-Results	19
             3.1.1   Arsenic(V) Retention	19
             3.1.2   Arsenic(III) Recovery	20
             3.1.3   Dead Volume in Oxidation Reactor Set-up	21
             3.1.4   Mixing Efficiency in Oxidation Reactor	22
      3.2    Chlorine Test Results	23
             3.2.1   Effect of pH on Chlorine	23
             3.2.2   Effect of Dissolved Manganese and Iron on Chlorine	25
             3.2.3   Effect of Sulfide on Chlorine	25
             3.2.4   Effect of TOC and Temperature on Chlorine	25
      3.3     Permanganate Test Results	25
             3.3.1   Effect of pH on Permanganate	26
             3.3.2   Effect of Dissolved Manganese and Iron on Permanganate	27
             3.3.3   Effect of Sulfide on Permanganate	27
             3.3.4   Effect of TOC and Temperature on Permanganate	28
      3.4    Ozone Test Results	28
             3.4.1   Effect of pH on Ozone 	29
             3.4.2   Effect of Dissolved Manganese and Iron on Ozone	30
             3.4.3   Effect of Sulfide on Ozone	30
             3.4.4   Effect of TOC and Temperature on Ozone	30
      3.5     Chlorine Dioxide Test Results	31
             3.5.1   Effect of pH on Chlorine Dioxide  	31
             3.5.2   Verifying Chlorine Dioxide Stock Concentration	33
             3.5.3   Fe(H) Oxidation with Chlorine Dioxide	34
             3.5.4   Increasing Stoichiometric Dose of Chlorine Dioxide	34
             3.5.5   Effect of Dissolved Manganese and Iron on Chlorine Dioxide	34
             3.5.6   Effect of Sulfide, TOC and Temperature on Chlorine Dioxide	34
      3.6    Monochloramine Test Results	35
             3.6.1   Effect of pH on Monochloramine  	35
      3.7    Solid-Phase Oxidizing Media (Filox)	36
             3.7.1   Filox: Effect of EBCT with High-DO	36
             3.7.2   Filox: Effect of pH with High-DO	39
             3.7.3   Filox: Effect of DO in Absence of Interfering Reductants	40
             3.7.4   Filox: Effect of Interfering Reductants	40
                    3.7.4.1   Low-DO Synthetic Water at l.SminEBCT	40
                    3.7.4.2  Low-DO Synthetic Water at 6.0 min EBCT	40
                                          VI

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                 3.7.4.3  High-DO Synthetic Water at 1.5 minEBCT	40
          3.7.5  Filox: Effect of Low Temperature in low-DO Synthetic Water	41
   3.8    UV Oxidation Results	41
          3.8.1  Unit 1—R-CanUltraviolet, 0.5 gpm	41
          3.8.2  Unit 2—Atlantic Ultraviolet, 1.0 gpm	...42
4.  Summary and Conclusions	43
5.  References	45
6.  Appendix A.   Preconditioning and Regeneration of IX Filter	48
          A.1    Manufacturer Recommended Preconditioning	48
          A.2   IX Filter Regeneration—UH Method	48
   Appendix B.   Testing Oxidation Reactor Set-up	49
   Appendix C.   Variable-DO Experiments	50
          C.I    High-DO Chemical Oxidation Experiments	50
          C.2    Low-DO Chemical Oxidation Experiments	51
   Appendix D.   Solid-Phase (Filox) Experiments	52
          D.I    Variable-EBCT and Variable-pH  experiments	52
          D.2   Low-DO Filox Experiments	52
   Appendix E.   Chlorine Oxidation Data	54
   AppendixF.   Permanganate Oxidation Data	57
   Appendix G.   Chlorine Dioxide Oxidation Data	60
   Appendix H.   Monochloramine Oxidation Data	63
   Appendix!    Ozone Oxidation Data	65
   Appendix J.    Filox Oxidation Data	68
   Appendix K.   UV Oxidation Data	70
   Appendix L.   Preparation of As(III) Stock Solution	72
   Appendix M.  QCData	73
                                      Vll

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                                   Figures
2-1    Schematic for Oxidation Reactor
2-2    Elevation View of Oxidation Reactor
2-3    Simplified Plan View of Oxidation Reactor
2-4    Schematic for Solid-Phase (Filox) Oxidizing Experiments
2-5    Schematic for UV-Oxidation Experiments
2-6    Typical Arsenic Calibration Curve
3-1    50 |ig/L-As(V) Retention by IX Filter
3-2    1000 ug/L-As(V) Retention by IX Filter
3-3    50 |ig/L-As(III) Recovery from IX Filter
3-4    1000 ug/L-As(III) Recovery from IX Filter
3-5    Estimation of Dead Volume in the Reactor-Set-up
3-6    Mixing Efficiency in Oxidation Reactor
3-7    Effect of pH on As(III) Oxidation with Free Chlorine
3-8    Effect of Sulfide on As(III) Oxidation with Free Chlorine
3-9    Effect of pH on As(IH) Oxidation with Permanganate
3-10   Effect of Sulfide on As(III) Oxidation with Permanganate
3-11   Effect of pH on As(III) Oxidation with Ozone
3-12   Effect of Sulfide on As(III) Oxidation with Ozone
3-13   Effect of TOC on As(III) Oxidation with Ozone
3-14   Arsenic(III) Oxidation with Chlorine Dioxide
3-15   Arsenic(III) Oxidation with Monochloramine
3-16   Effect of EBCT on Filox Media
3-17   Filox Media Equilibration
3-18   Effect of pH on As(III) Oxidation with Filox Media
Page

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                                      Vlll

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                                  Tables
2-1   Composition of Synthetic Test Water
2-2   Interfering Reductants
2-3   Previous EPA Standards
2-4   Analytical Methods
3-1   Free Chlorine Experiments
3-2   Permanganate Experiments
3-3   Ozone Experiments
3-4   Chlorine Dioxide Experiments
3-5   Chlorine Dioxide Assays
3-6   Monochloramine Experiments
3-7   Filox Experiments
3-8   UV Oxidation Experiments
E-l   Residual As(III) Cone, vs Time for Chlorine Experiments
F-l   Residual As(III) Cone, vs Time for Permanganate Experiments
G-l   Residual As(III) Cone, vs Time for Chlorine Dioxide Experiments
H-l   Residual As(III) Cone, vs Time for Monochloramine Experiments
1-1    Residual As(III) Cone, vs Time for Ozone Experiments
J-l    Residual As(III) vs Effluent As Concentration for Filox Experiments
K-l   Residual As(III) Cone, for UV Experiments
M-1   Results of QC Analysis
M-2   Results of WS Standards Analysis
M-3   Recoveries of Spiked WS Standards
Page

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                                      IX

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                    Abbreviations and Acronyms
AWWA      American Water Works Association
DO          Dissolved Oxygen, mg/L
DPD         N,N-diethyl-p-phenylenediamine
EBCT        Empty Bed Contact Time (media volume/flow rate), min
EPA         US Environmental Protection Agency
FIAS         Flow Injection Analysis Hydride Generation System
GFAAS      Graphite Furnace Atomic Absorption Spectrophotometer
GPM         Gallons/min
HOPE        High Density Polyethylene
IR           Interfering Reductant
MCL         Maximum Contaminant Level
MDL         Method Detection Limit
MPT         Nominal Pipe Thread
NRMRL      National Risk Management Research Laboratory
PAO         Phenyl Arsenic Oxide
PRV         Pressure Relief Valve
QA          Quality Assurance
QAPP        Quality Assurance Project Plan
QA/QC      Quality Assurance/Quality Control
SDWA       Safe Drinking Water Act
SM          Standard Methods
SR          Stoichiometric Ratio, ug oxidant/ug reductant
TOC         Total Organic Carbon
UH          University of Houston
UV          Ultraviolet
WAL         Work Assignment Leader
WAM        Work Assignment Manager

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                           Acknowledgments
This research was funded by the US EPA under EPA Order Number 8C-R311-NAEX.
The authors are grateful for the technical and administrative support of Tom Sorg, US
EPA Research Engineer.  Anthony  Tripp,  a doctoral candidate at the University of
Houston is gratefully acknowledged for serving as the QA/QC  Officer  and  for his
considerable technical assistance throughout this study.
                                    XI

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                               1. Introduction
1.1    Background
Arsenic  (As) is  a metalloid that is
naturally present in drinking water in a
variety of forms (organic and inorganic),
oxidation states,  and valences (Ferguson
and  Gavis,  1972).   Inorganic  arsenic
predominates in drinking water and is
present as arsenate (As(V)) and arsenite
(As(ni)).  Under pH conditions  of 6-9,
As(V) exists as an anion while As(DI) is
fully   protonated  and  exists  as   an
uncharged   molecule   (Clifford   and
Zhang, 1994).

While any future revisions in the arsenic
MCL will likely target total arsenic, the
speciation  of arsenic  (HI  or  V)  is
significant  because  of  differences  in
arsenic removal  efficiencies  by various
treatment techniques.  As(V) is generally
more efficiently removed than As(m),
which is  poorly removed using treatment
processes  such   as   ion   exchange
(Clifford, 1999,  Clifford et  al.,  1998a,
1997), iron  coagulation  followed  by
microfiltration (Clifford et  al.,  1998b;
Ghurye et al., 1998; Hering et al., 1996a,
1996b),    and    activated    alumina
adsorption    (Clifford,   1990,   1986;
Clifford  et  al.,  1998b; Hathaway  and
Rubel, 1987).  Hence, for drinking water
supplies      containing     significant
concentrations of As(DI),  preoxidation
of As(ni)  to As(V) is mandatory for
high arsenic removal.
1.1.1  Previous Studies
Frank  and Clifford  (1986)  studied the
oxidation of  As(ni)  to  As(V) using
chlorine, monochloramine  and oxygen.
They determined that the  oxidation of
As(IH) by chlorine was very rapid.  A
1.0 mg/L free chlorine dose was able to
oxidize 100 |ig/L As(m) in less than 5
seconds.  Monochloramine was able to
oxidize only a  fraction of  the  initial
As(ni)   present,   possibly   because
chlorine injected in the presence of an
excess    of   ammonia    to    form
monochloramine, was  responsible for
As(HI)     oxidation    rather    than
monochloramine. Oxygen was found to
be  ineffective   for  As(HI)  oxidation.
Amy  et al. (2000) studied the use of
chlorine, ozone  and  permanganate for
As(ni)  oxidation.    This  study  used
excess  (over As stoichiometry)  ozone
and stoichiometric doses of chlorine and
permanganate.    All  three   oxidants
proved  effective  although  less  than
100%  As(IH) oxidation was  obtained
with chlorine and permanganate.  This
was probably the result of NOM (0.2 -
2.3  mg/L)  presenting  a   competing
oxidant demand. Although higher doses
of chlorine and  permanganate were not
tested, the authors concluded that the
provision    of    these   oxidants   in
stoichiometric excess  would  result in
complete conversion of As(m) to As(V).

Oxidation of As(IH)  to  As(V) by solid-
phase  oxidants  such  as  birnessite (8
MnOa)  has  also  been  reported  by
Oscarson  et al. (1983), Moore  et al.
(1990), Driehaus (1995), and Scott and
Morgan (1995).  These studies generally
concluded    that  birnessite   directly
oxidized As(IH) to   As(V)  through  a
surface  mechanism    and    that  the
adsorption of As(ni) to the oxide surface
was the rate-limiting step.  Additionally,
Scott and Morgan (1995) concluded that
dissolved  oxygen had no  effect on the

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rate of the oxidation reaction. However,
they  did  not  study  the  effect  of
interfering    reductants    on   As(m)
oxidation. As will be shown later in this
report, we observed no effect of DO on
As(III)  oxidation   in  the absence  of
interfering reductants. We did, however,
observe  that the  DO   level  had  a
significant detrimental  effect on As(ni)
oxidation in  the presence of interfering
reductants.

As(ni) oxidation has also  been  studied
by other researchers using a variety of
techniques   including   electrochemical
oxidation (Catherine,  1967),  oxidation
by electrogenerated iodine (Johnson and
Bruckenstein,   1968),    oxidation   by
peroxodisulfate (Gupta, et al., 1984 and
Nishida and Kimura, 1989), oxidation by
perchloric  acid  (Everett  and  Skoog,
1971), oxidation by chromic acid (Sen
Gupta  and   Chakladar,   1989),   and
oxidation    by    hexacyanoferrate(m)
(Mohan et  al., 1977).   These methods
were  considered unsuitable for  As(ni)
oxidation in  drinking water and hence,
were not investigated in this study.

1.2    Oxidants Evaluated

Oxidation experiments were  performed
in the aqueous phase via the addition of
oxidants such as chlorine or ozone to a
solution  containing   As(IH)  or   by
contacting     the    As(nT)-containing
solution with  a solid-phase oxidant.  A
solid-phase oxidant is typically a media
that has the oxidant immobilized on its
surface and such media may be used in
packed columns  through  which  the
As(IH)-containing solution is passed at a
specified flow rate or empty bed contact
time (EBCT). The use of UV radiation
to oxidize As(ni)  to  As(V) was also
studied at a wavelength of 254 nm, using
two    commercially   available    UV
disinfection units.
1.3    Chemical Oxidant
       Stoichiometry

Reaction stoichiometries  of the various
chemical  oxidants   with  As(in)   and
potentially  interfering  reductants  are
summarized below.

1.3.1   Chlorine

For AsdED:
H3AsO3 + NaOCl
Na+ + Cr + H+
H2AsO4" +
(1)
                50
In reaction  (1),  one mole of  As(m)
requires  1  mole  of NaOCl, which is
equivalent to one mole of Cfe.   A
|ig/L As(m) solution contains 0.667
As(EI)/L  (AW   of   As   =   74.92).
Therefore,      the      stoichiometric
requirement  of chlorine is 0.667 uM/L
or 47.4 jag Cb/L,  and the stoichiometric
ratio (SR) of chlorine needed to oxidize
As(m) is 0.95 jig Cla/ng As(DI).

SR = 0.95 ug CVug As(ffl)

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ForFedl):
2Fe(OH)3 (s) +Cr +5H+

SR = 0.64 jig Cl2/|ag Fe(E)

For Mn(IT):
                                 (2)
MnO2 (s) +C1" +3IT

SR = 1.29 |ag Cl2/|ag Mn(E)

For Sulfide:
                                 (3)
MS' + HOC1
+ CF + H20
                                 (4)
SR = 2.21 jig Cl2/|ag S2'
pH Range = 5-9
Optimum pH = 9.0
                                 (5)
SR = 8.86 |ag Cl2/|ag S2'
pH Range = 5-9
Optimum pH = 6.0

The chemistry of the oxidation of sulfide
is  extremely complex.  Reportedly, the
oxidation  proceeds   to  form  either
elemental sulfur, sulfate, or both (White,
1986).   Equations  (4) and  (5) for the
oxidation of sulfur with chlorine  were
obtained  from  White   (1986).  The
oxidant doses  used in the presence of
sulfide were either three- or 10-times the
stoichiometric   requirement  based  on
As(m)   alone.   Since   the   As(ni)
concentration in the presence of
                         interfering reductants  such as  sulfide
                         was  always  50  |ag/L, the  resultant
                         oxidant  doses used were low in relation
                         to the sulfide concentrations of 1.0 and
                         2.0 mg/L, Oxidation reactions,  similar
                         to  equation  (4)  for  chlorine dioxide,
                         permanganate,       ozone,       and
                         monochloramine were derived based on
                         oxidation of sulfide to elemental sulfur
                         only.

                         1.3.2  Permanganate

                         For AsdTf):

                         3H3 AsO3 + 2MnO4- -» 3H2AsO4" +  (6)
                                          SR = 1.06 |ag MnO47|ag As(HT)

                                          ForFeOD:

                                          3Fe2+ + Mn04- +7H2O -»
                                          3Fe(OH)3 (s) + MnO2 (s) + 5H+

                                          SR = 0.71 jag MnO47|ig Fe(II)

                                          For Mn(TD:

                                          3Mn2+ + 2MnO4- +2H2O -»•
                                          5MnO2 (s) + 4H+

                                          SR = 1.44 |ag MnO47|ag Mn(H)

                                          For Sulfide:

                                          3HS- + 2MnO4- + 51^^ 3S°i +
                                          2MnO2(s) + 4H20

                                          SR = 2.48 |ag MnO47ug S2'
                                                         (7)
                                                         (8)
                                                         (9)

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1.3.3   Ozone

For AsOID:

H3AsO3 + O3 -» H2As04" +
O2 + H"(@pH6.5)

H3As03 + 03 -> HAs042' +
O2 + 2H+(@pH8.5)

SR = 0.64 ug O3/ug As(m)

ForFeOD:

2Fe2+ + 03 (aq) + 5H2O ->
2Fe(OH)3 (s) + O2 (aq) + 4H+

SR = 0.43ugO3/ugFe(II)

ForMnOD:
Mn02(s)

SR = 0.88 jig O3/ug Mn(E)

For Sulfide:
HS" + O3 (aq) + H"
O2 (aq) + H2O

SR=1.50ug03/ugS2
                               (10)
                               (12)
                               (13)
                               (14)
1.3.4   Chlorine Dioxide
Oxidation by chlorine dioxide can occur
via a  1-  or 5-electron transfer with the
conversion  of C1O2 to C1O2" or  Cl",
respectively. Knocke (1990) determined
that chlorine dioxide oxidized Mn(n) via
a 1-electron transfer whereas it oxidized
Fe(H) via a 5-electron  transfer.  When
calculating   an   appropriate  chlorine
dioxide dose for As(m), a conservative
1-electron transfer was assumed.
However,   theoretical   stoichiometric
ratios for both 1-and 5-electron transfer
mechanisms are shown below.
             •%
For As(m):
                                                           H2O-»-H2AsO4- +
                                          2C1O2" + 3H+ (1-electron transfer)  (15)

                                          5H3As03 + 2C102 + H20 -»• 5H2AsO4"
                                          + 2C1" + 7H1" (5-electron transfer)   (16)
SR=1.80 u
transfer
SR=0.36 u
transfer

For FeOD:
           ClO2/|ig As(HI) for 1-electron

           ClO^ug As(m) for 5-electron
                                               + C1O2 + 13H2O
                                          5Fe(OH)3(s) + Cr
                                          SR=0.24 ug
                                          transfer

                                          For Mndl):
                                (17)


                    Fe(E) for 5-electron
     + 2C1O2 + 2H2O -»
MnO2 (s) + 2C1O2- + 4H+
                                                                          (18)
                                          SR=2.45 ug CICVug Mn(E) for 1-electron
                                          transfer
For Sulfide:

HS' + 2C1O2 -»• S°
1 electron transfer
                   2C102'
                                                                          (19)
                                          5HS' + 2C1O2 + 3ET -> 5S°i +     (20)
                                          2C1" + 4H2O  (5 electron transfer)
                                                                2"
                                          SR = 4.21 ug ClO^ug S" for 1-electron
                                          transfer
                                          SR = 0.84 ug ClO2/ug S2" for 5-electron
                                          transfer

-------
Chlorine dioxide was prepared according
to the general  procedure described  in
Standard    Methods     with    two
modifications (White, 1986) to increase
the  yield  of  chlorine   dioxide  and
decrease the background  concentrations
of  chlorine   and   chlorite.    These
modifications  were  (a)  doubling  the
reagent   concentrations   of   sodium
chlorite  and sulfuric acid and (b) pre-
cooling the receiving solution


1.3.5  Monochloramine
For AsCIII):

H3As03 + NH2C1 + H2O
HAsO42"
                                (21)
SR = 0.69 ug NH2Cl/ug As(ffl)

For Fe(n):

2Fe2+ + NH2C1 +6H2O ->•
2Fe(OH)3 (s) + NH4+ + Cl' + 4IT

SR = 0.46 |ag NH2Cl/ug Fe(H)

For MnOD:

Mn2+ + NH2C1 +2H2O ->
                                (22)
MnO2 (s) + NHU+ + Cl" + 2H+

SR = 0.94 |ag NH2Cl/ug Mn(H)

For Sulfide:

S2" + NH2C1 +2BT -»
sU + NHU+ + cr
                                (23)
1.4    Research Objectives

The overall objective of this study was
to determine the effectiveness of five
chemical   oxidants,   a   solid-phase
oxidizing  media, and UV  radiation  in
oxidizing  As(m)  to As(V)  under  a
variety  of  environmental   conditions.
Potentially interfering reductants such as
dissolved  manganese, dissolved iron,
sulfide and TOC were studied as they
are  typically   present   in   arsenic-
contaminated  waters.    The   specific
objectives of this study were as follows:

(1)  Study  the  effectiveness  of  (a)
chlorine (b) permanganate (c) ozone, (d)
chlorine  dioxide, (e) monochloramine,
(f) solid-phase oxidizing media and (g)
UV  radiation   (@254  nm)  for  the
oxidation of As(DI) to As(V).

(2)  Determine the effect of pH, in the
range of  6.3-8.3,  on the  oxidation  of
As(m) to As(V).

(3)  Determine the effect of potentially
interfering    reductants     including
dissolved  iron,  dissolved  manganese,
sulfide, and TOC, and the effect of low
temperature  (5  °C) on  the  oxidation
process.
                                (24)
SR= 1.61 |ag NH2Cl/ugS
                       2-

-------
                        2. Materials and Methods
2.1    Synthetic    Test    Water
Composition
The composition of the  synthetic test
water  used to  perform the  oxidation
experiments is shown in Table 2-1. The
synthetic test  water  contained most of
the common ions found in surface and
ground waters.
                  Table 2-1.  Composition of Synthetic Test Water.
Cations
Na+
Ca2+
Mg2*


Total
meq/L
3.3
2.0
1.0


6.3
mg/L
75.9
40.2
12.2


128.3
Anions
HC03"
S042'
cr
Silicate as
SiO2

meq/L
3.0
0.5
2.5
0.3

6.3
mg/L
183.0
24.0
88.8
20.0

315.8
Calculated TDS = 128.3 + 315.8 - 93 mg/L (loss of H2CO3 during evaporation) = 351.1 mg/L
2.2  Interfering Reductants
Synthetic  water  was  amended  with
potentially  interfering  reductants  and
studied for their ability to affect As(III)
oxidation.    The  various  interfering
reductants studied are listed in Table 2-2
along with their concentrations.
                         Table 2-2. Interfering Reductants.
              Interfering Reductant
 Concentration (mg/L)
              Manganese (Mn(II))
              Iron (Fe(II))
              Sulfide (S2-)
              Total Organic Carbon (TOC)
         0.2
       0.3, 2.0
       1.0,2.0
       1.4-6.9
2.3    Speciation Method
A 3M  Empore anion  exchange  filter
(hereafter referred to simply as IX filter)
was used to speciate As(III)/As(V). The
filter  was preconditioned according to
the   manufacturers   recommendations
(See  Appendix  A).   Following  the
manufacturers          preconditioning
procedure,  the  IX  filter  was  further
conditioned     (UH     Method—See
Appendix A) by acid regeneration and 1
M NaCl treatment to ensure  that  all
exchange sites on the filter were in the
chloride form prior to initial use or reuse
of the filter.  Finally, excess 1 M NaCl
was  rinsed from the filter with 0.005 M
NaCl.     Analytical  reagent   grade

-------
chemicals  were  used  throughout  the
procedure.

2.4    Chemical        Oxidation
Experiments
The procedures developed to design and
operate  the   oxidation   reactor   are
described here. The design of the reactor
is described in detail in Section 2.4.1.
Before the oxidation reactor could be
used  to   study  any  of the chemical
oxidants, the reactor set-up  was tested to
(a) prove that the IX filters used in-line
with   the  oxidation  reactor   could
efficiently  remove  (>90%   retention)
As(V),  (b)  prove that  the  IX filters
caused  no  inadvertent  oxidation  of
As(III) (>90% recovery), (c)  determine
the dead volume in the reactor set-up,
and  (d)   determine  the efficiency  of
oxidant mixing in the reactor.  Section
2.4.2  describes these  tasks and   the
procedures  used  to   achieve   them.
Section 2.4.3  describes the  procedures
used  during  routine  operation  of  the
oxidation reactor in either high-  or low-
DO synthetic  water.   Finally,  Section
2.4.4   discusses   chemical   oxidation
experiments  performed  to  study   the
effect of TOC and low temperature.
The procedures  described  in  Sections
2.4.2 - 2.4.4 have some common steps,
irrespective  of the  objective  of  the
experiments.    At  the  same  time,
depending on the objective, there were
certain   inherent  differences   in   the
procedures used to operate the  reactor.
For example, a larger sample  volume
was desired when testing the reactor set-
up  to determine the capacity of the IX
filter whereas a smaller sample  volume
was   required    during    oxidation
experiments. However, in the interest of
thoroughness, and at the risk of being
repetitive, the procedures are described
exactly as they were performed.

2.4.1 Reactor Design
The schematic design of the oxidation
reactor is shown in Figure  2-1  and  the
elevation and plan views are shown in
Figures  2-2 and 2-3, respectively.   To
simplify  the  drawing,  the side port  for
the pH probe is not shown in the plan
view. The reactor was equipped with a
pH probe, a nitrogen gas Met, a pressure
relief valve,  a septa for injecting  the
oxidant, and an outlet port.  The reactor
was  made  of  clear  Plexiglas  and
fabricated at the University of Houston
Machine Shop.

-------
                                     Inject oxidant
                                     @t=0
  Pressure Relief
  Valve
 50 ug As(III)/L
 1 L Total Volume
  Magnetic Stirrei
     On Bottom
         Filter
         Holder
                                        Arsenic Oxidation
                                        Reactor
60
    50
                        Flow Rate = 5-25 mL/min
                     0.22 jam filter atop
                     3M Empore Filter
                          Fraction Collector/Sampler
        40
30
                 20
                      10 sec
 Figure 2-1. Schematic for Oxidation Reactor.

-------
l/2"-thick, Pressure
5"-square flanges , 1/8"NPT(F) , Relief Valve


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1/2" NPT(F)
for pH/Temp
probe

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1/2" Initial Liquid
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                             3"
Figure 2-2.  Elevation View of Oxidation Reactor. Scale 1:1

-------
1/4" holes
                              5"
  Figure 2-3. Simplified Plan View of Oxidation Reactor.  Scale 1:1
                               10

-------
2.4.2  Testing Reactor Set-up
2.4.2.1 Arsenic(V) Retention
To  ensure that >  90%  As(V)  was
retained  by the  IX filter, synthetic test
waters  containing  50  and  1000  jj,g
As(V)/L were passed through the IX
filter.     After  the  passage  of   a
predetermined  volume  of  the As(V)-
containing solution, the IX  filter was
regenerated  and  reused  for  further
testing.  In all, six sequential runs were
performed.  The first run was performed
using a fresh IX filter, whereas, the next
five were performed using a regenerated
IX filter.  The procedures used during
testing are described in Appendix B.

2.4.2.2  Arsenic(III) Passage
To ensure that  > 90%  As(III) passed
through  the IX filter, synthetic waters
containing 50  and 1000 |j.g As(III)/L
were tested. The procedures used for the
As(III)-passage  experiments  were  the
same as those used for  the  As(V>
retention   experiments   described   in
Appendix B.

2.4.2.3 System Dead Volume
The dead volume in the sampling system
consisted  of the  volume  of tubing
connecting the oxidation reactor and the
filter holder plus the dead volume hi the
filter holder itself.   The dead  volume
inside the filter holder was necessary in
order   to    evenly    distribute   flow
throughout the surface of the filter. This
dead volume had to be wasted before the
contents  of the reactor could be sampled.
The dead volume could not be calculated
directly   because  of   problems   in
calculating the void  space  within  the
filter holder.
The dead volume of .the reactor set-up
was estimated by introducing  a step-
increase in  arsenic concentration  (from
0-1000 |ig/L) into the well-mixed reactor
and then plotting  the  effluent arsenic
concentration vs volume passed through
the IX filter.   Specifically, 500 mL of
arsenic-free synthetic  test water  was
placed in the reactor.  The reactor was
pressurized and the necessary volume of
As(III) solution was injected  into  the
reactor  at  t   =  0 to   give   a  final
concentration of 1000 fig As(III)/L. The
reactor  outlet  valve   was   opened
immediately at t  = 0  (flow rate  =  5
mL/min)  and  samples  (0.5  mL)  were
collected in the fraction collector  at an
interval of 6 seconds.

2.4.2.4 Oxidant Mixing Efficiency
When  the oxidant  was introduced into
the reactor,  it  was important that  it
mixed  instantaneously with the contents
of the  reactor,  so  that inefficiencies in
mixing  did not   lead   to  erroneous
conclusions  about the kinetics of As(III)
oxidation.  Mixing of the oxidant was
assumed to  be adequate if As(III) was
immediately oxidized by a large excess
(200-fold) of chlorine injected into  the
mixed   reactor.    To   determine   the
efficiency  of  oxidant  mixing  in  the
reactor, a 10 |j,g/L  As(III) solution was
placed in the reactor. At time t = 0, a
chlorine dose of 2 mg/L (200-fold excess
over stoichiometric requirement)  was
added to the reactor. The first 4 mL of
reactor effluent was wasted as  dead
volume and the rest was collected in 2-
mL increments at 6 second intervals
(flow rate = 20 mL/min).
                                        11

-------
2.4.3   Chemical Oxidation Conditions
Each  initial  As(DI) concentration was
tested with at least three concentrations
of each oxidant: three-, ten-,  and one--
hundred times the stoichiometric amount
required  to oxidize the As(]H)  initially
present.   Two levels  of oxidant were
used  in  the presence  of  interfering
reductants: ten times  based  on  As(]H)
concentration alone, or a higher dose of
three-times-stoichiometric   based   on
As(IE[) plus  the interfering  reductant.
However, if the lower dose successfully
oxidized greater than 90% of the As(ni)
initially  present, then higher  oxidant
doses were not tested.   Initial  As(HI)
concentration  was  50   ug/L,  unless
mentioned otherwise.   Chlorine  was
dosed   in   the    form   of   sodium
hypochlorite  (NaOCl) and reported  as
mg/L chlorine.

All the  chemical oxidation experiments
(as  well   as   the   UV   oxidation
experiments)   were    performed   in
duplicate.  One control experiment was
performed for each   set  of  duplicate
experiments.    The control experiment
was   performed    under    identical
conditions as the duplicated experiments
with the exception  that no oxidant was
added.       Therefore,   the   control
experiment   established  As(ni)  losses
due to  adsorption  to  the  walls  of the
reactor   or  other  parts,   and  also
established any potential As(m) losses
due to  reaction with  the  interfering
oxidants  such  as  sulfide  or  dissolved
iron.  During the  course of this study,
several  such control  experiments were
performed and no  As(m) losses were
observed   as  a   result  of  arsenic
precipitation        or       adsorption.
Furthermore,  all  the  oxidation  data
indicated  that when sulfide was present,
more  rather  than  less  As(IEI)  passed
through the IX filter, which suggests that
sulfide  did not precipitate and remove
As(m).

2.4.3.1 pH of Operation
For all of the oxidants studied, the extent
of As(DI) oxidation was determined for
a pH range of 6.3 - 8.3.  The effect of
interfering reductants was  only  studied
at pH 8.3 because previous studies had
suggested  that  typical As-containing
groundwaters would have pH values of
8.3 ± 0.6 (Clifford and Lin, 1986, 1991;
Clifford,  1990;  Clifford  and  Zhang,
1994;  Clifford, et  al, 1991,  1998a,
1998b; Ghurye, et al., 1998, 1999). This
may,  however, not be  true for  all As-
containing waters.

2.4.3.2 High- andLow-DO Experiments
Chemical  oxidation   experiments,  in
which the effect of dissolved iron on
As(in) oxidation was studied, had to be
performed in very low DO  waters (< 0.1
mg/L  DO)  because  dissolved   iron
(Fe(E[))  is very easily  oxidized in  the
presence of DO at greater than neutral
pH  values  (Knocke  et   al.   1990).
Additionally,    chemical     oxidation
experiments     with    sulfide-spiked
synthetic water were  also performed in
the absence of DO because sulfide can
be oxidized by DO.  Dissolved  oxygen
did not  present a problem with the other
interfering reductants studied.

The  high-DO   chemical   oxidation
experiments were  performed by raising
the DO of the synthetic water to near-
saturation, by sparging with air (from the
lab air-distribution system)  through a
diffuser  for about 15  minutes.   The
procedures    used     during     these
experiments are described  in Appendix
C.   The low-DO chemical  oxidation
experiments were performed by sparging
                                        12

-------
extra dry grade nitrogen gas (containing
approx. 3 ppm ©2) through the synthetic
water to reduce the DO to < 0.1 mg/L as
measured  by  a lab DO meter.   The
procedures  used during the  low-DO
experiments are described in detail in
Appendix C.

2.4.3.3 Oxidation Experiments with TOC
In order to determine the effect of TOC
on the  oxidation of As(IH) with the five
chemical   oxidants,   untreated  Lake
Houston water (not previously exposed
to oxidants) was  filtered sequentially
through a 8.0, 0.45, and 0.22 urn filters
and  then stored at  4 °C.  Prior to  an
experiment, the filtered  Lake Houston
water  was allowed  to  come to room
temperature (-24 °C).   This  was either
used   directly   by   spiking   with   an
appropriate amount  of As(IH) or diluted
with synthetic  water to  yield  a water
with 2.1 mg/L  TOC as in  the  ozone
experiments.   The TOC in the filtered
Lake   Houston   water   was    not
characterized.

2.4.3.4 Low   Temperature    (5    °C)
Oxidation
A limited number  of experiments were
performed at 5 °C to determine the effect
of low  temperature on As(DI) oxidation.
Only those oxidants that were successful
in oxidizing As(ni) to As(V) were tested
at  low  temperature.     The  general
procedure   for    performing   these
experiments was the same  as  the other
chemical    oxidation     experiments.
Additionally,  the   oxidation   kinetics
reactor, filled with 500 mL of synthetic
water containing 50 [ag/L  As(IE), was
refrigerated to a temperature of 5 ± 1 °C.
Upon attaining the required temperature,
the reactor was quickly  removed from
the refrigerator and dosed with the
appropriate   oxidant.      The   entire
experiment  was  usually completed in
less than  6  minutes from  the time the
reactor    was   removed   from   the
refrigerator.     At  the  end  of  the
experiment,     the    reactor    water
temperature  was usually in the range of
5-6 °C.

2.5   Solid-Phase Media (Filox)
Experiments
The solid-phase oxidizing media used in
this project was Filox-R™  (Matt-Son
Inc, Barrington,  IL), which is typically
used for  iron,  hydrogen  sulfide  and
manganese-  removal.   The  gray-black
granular Filox media  (12  x 40  mesh,
bulk density 114 lbs/ft3)  containing 75-
85%  manganese  dioxide,  reportedly
utilizes   an  oxidation-reduction   and
filtration  process   for   removal   of
dissolved  iron,  hydrogen  sulfide  and
manganese.  In this study, the media was
used to  oxidize As(m) to As(V) under a
variety of conditions.

2.5.1 Pretreatment of Filox Media
Approximately  100  mL of  the  Filox
media was  backwashed  with synthetic
water (of the composition in Table 1 and
without  any  As(DI)) in a 1  in.-i.d. glass
column  at  a flow rate of 285 mL/min (14
gal/min  ft2)  for 30  minutes.    After
backwashing,  12  mL of  media was
transferred to a 1 cm-i.d. glass column.
The bed depth was 6 inches (15 cm) and
the  flow  rate   was  varied  from 16
mL/min (EBCT  of 0.75  min)  to  2
mL/min  (EBCT  of   6.0  min).    A
schematic  for the Filox experiments is
shown in  Figure 2-4.   All  of the Filox
experiments  were performed with the
same 12 mL of media,  which was not
treated in  any way except  for periodic
backwashing.
                                       13

-------
                 Synthetic
                    Test
                  Solution
                                                  1-cmi.d
                                               Glass Column
                                                   -Filox Media
        IX Speciating
        Filter Holder
                                         As(IE)
                              2-16 mL/min
               Feed Reservoir
         Figure 2-4. Schematic for Solid-Phase (Filox) Oxidizing Experiments.
2.5.2   Preparation of Low- and High-
DO Synthetic Water
The high-DO synthetic water (8.2 mg/L
DO)  was  prepared  by  sparging  air
through synthetic  water for about 15
minutes prior to  passage  through  the
Filox column.   The low-DO synthetic
water (0.1  mg/L DO) was prepared by
sparging extra-dry-grade nitrogen for 30
minutes through 500 mL  of synthetic
water (or filtered Lake  Houston water)
contained in the oxidation reactor.

2.5.3   Procedure  for  Variable-EBCT
and Variable-pH Experiments
The  procedures  for the variable-EBCT
and the variable-pH Filox experiments
were identical except for the pH of the
synthetic water.   All  of the variable-
EBCT experiments were performed  at a
pH of 8.3 whereas during the variable-
pH experiments, the pH of the synthetic
water was  varied at 6.3, 7.3, and 8.3.
The procedures are described in detail in
Appendix D.
2.5.4   Procedure to Stabilize Arsenic
Removal by Filox Media
During the variable-EBCT experiments,
it was observed that the Filox media, in
addition to oxidizing As(IEI)  to As(V),
also removed arsenic.   To  enable  the
media to come to equilibrium with the
As(IH)-containing   water   to   better
represent what would occur in a Filox
column  in  actual  field  use,   pre-
equilibration   of   the   media   was
attempted.    To  achieve equilibrium,
2000  BV  (24 L) of pH  8.3  synthetic
water containing  50 |ag/L As(in) was
                                       14

-------
passed through the media at an EBCT of
0.75 min.

2.5.5  Procedure for  Low-DO Filox
Experiments
As   mentioned  earlier,  the  low-DO
synthetic  water was  prepared in  the
oxidation reactor in the same manner as
during    the    chemical    oxidation
experiments.    The low-DO synthetic
water was then pumped directly from the
reactor  to the  Filox  column.    The
   procedures used are described in detail
   in Appendix D.

   2.6   UV Oxidation Experiments
   2.6.1   Unit 1:   0.5 gpm UV Sterilizer
   Unit
   A schematic  for  the  UV  oxidation
   experiments is shown in Figure 2-5.  The
   experimental   set-up  for   the   UV
   experiments was the  same as  for the
   Filox experiments except that the Filox
   column was substituted with a UV unit.
                To Vacuum
                                    IX Speciating
                                    'Filter
Outlet
                        Vacuum Filtration
                           Apparatus
                                        Inlet
                       Synthetic
                         Test
                       Solution
           UV Apparatus
                                   12-288 mL/min
                     Feed Reservoir
                Figure 2-5. Schematic for UV-Oxidation Experiments.
Unit 1 was a 0.5 gpm unit from R-Can
Environmental Inc. (Canada), equipped
with a low-pressure mercury lamp with
an advertised lamp intensity of 32,000
uw/cm2  at  254  nm.   The  unit was
mounted vertically with the electrical
connections to  the lamp  on  top  to
prevent accidental water spillage from
damaging the sterilizer assembly.  As
   seen from Figure 2-5, the lower port was
   chosen as the inlet and the upper port the
   outlet. A 4-L HDPE container was filled
   with synthetic water containing  50 |iig
   As(III)/L.  Before the UV lamp was
   turned on, the synthetic test water was
   pumped  through the  inactive  sterilizer
   assembly at 288 mL/min (residence time
   1.0  min), and the  effluent  from the
                                       15

-------
sterilizer unit was  collected, speciated,
and analyzed for As(III). This served as
a  control for the  UV experiments to
ensure  that  the  act of  pumping the
synthetic  water  through the  inactive
sterilizer  unit  did  not   cause   any
inadvertent As(III) oxidation.  The flow
was then switched to the lowest setting
corresponding to the highest  residence
time employed.  The lamp was switched
on and allowed  to stabilize for  30
minutes, which is much longer than the
3-5  minutes  recommended  by  the
manufacturer. At the end of 30 minutes,
three  consecutive 50-mL samples  were
collected,  speciated,  and analyzed  as
As(III). The flow was then Increased to
the next flow rate and 600  mL (> two
times the void space of 288 mL in the
sterilizer  assembly)  of  effluent  was
wasted  before three consecutive 50-mL
samples were collected, speciated and
analyzed for unoxidized As(III).  The
above procedure was repeated until  all of
the flow  rates  were  studied.    The
temperature  of the water exiting the UV
unit was not measured.

2.6.2   Unit 2:  1.0 gpm  UV Sterilizer
Unit
Unit 2 was a 1.0 gpm unit from Atlantic
Ultraviolet Corp., equipped with a low-
      pressure   mercury   lamp   with   an
      advertised  lamp  intensity  of  41,200
      uw/cm2 at 254 nm.  The experimental
      set-up and operation were similar to the
      one used for Unit  1.  The void space in
      Unit  2 was larger, 486 mL, compared
      with 288 mL for Unit 1.

      2.7   QA/QC
      2.7.1  Arsenic Standards
      Four standards  from an  arsenic AAS
      standard  solution  (Aldrich   Chemical
      Company,  Inc.)  containing   1000  jag
      As/mL  were  employed  for  routine
      arsenic analysis.  The concentrations of
      these "WAL standards" were 1.3, 2.8,
      6.8, and 11.0 |4,g/L.  Independently, the
      QA/QC Officer for this project, prepared
      four  separate arsenic standards,  which
      served  as  internal  QA/QC  standards.
      Two such sets of four QA/QC standards
      were prepared  by the QA/QC  Officer
      during the duration of this project.   In
      addition to the standards prepared at UH,
      we utilized four previous "Water Supply
      Laboratory   Performance   Evaluation
      Study" standards.  These standards were
      preserved in a 0.2 M nitric acid solution.
      These WS  standards and their EPA-
      reported true  concentrations  are   as
      follows:
                        Table 2-3. Previous EPA Standards.
                      Standard        Year  '   EPA-Reported
                                               Concentration
                                                   Hg/L
                      WS037
                      WS038
                      WS040
                      WS041
1996
1997
1997
1998
 49.3
 83.1
102
 65.6
                                       16

-------
A stock  solution containing 88.0 mg/L
As(III) was prepared according to the
procedure described in Appendix L.

2.7.2  Routine Arsenic Analysis
Routine arsenic analyses were performed
by the WAL during the course of this
project.   Each  batch  of samples  was
analyzed  according to  the  following
sequence:
(1) A prep blank (prepared the same as a
routine sample with the exception that
reagent grade water was substituted for
the  arsenic   sample)  was   analyzed
followed by the four  WAL  standards.
Then, one of the four QA/QC standards
was analyzed.  This sequence is hereafter
referred to as a "set of standards."
(2) Following measurement of the set of
standards, no more than 20 samples were
analyzed.  This was followed  by the
analysis  of the set of standards.   Once
again,  no more  than 20 samples  were
analyzed   followed  by  the  set  of
standards,  and so  on  until all of the
samples had been analyzed.
(3) Then each of the four WS standards
was  measured.    Each  of the  WS
standards was  spiked with  2.0  ug/L
arsenic and measured to  calculate spike
recoveries.
(4)  Finally, the set  of standards was
measured to complete the analyses.

2.7.3  Arsenic Calibration Curve
A calibration curve was developed for
arsenic based on the prep blank and the
four  WAL  standards.   The  QA/QC
standard  was thus  excluded from the
points  contributing to  the  calibration
curve  and  its  concentration  was
calculated on the basis of the calibration
curve.    Figure  2-6 shows a  typical
calibration   curve  with  the  QA/QC
standard  included for comparison.   In
Figure 2-6, each standard was measured
eight times.   The  concentration of the
QA/QC standard in Figure 2-6 was 5.97
ug/L   and   the  average   of  eight
measurements  of the QA/QC standard
was 5.91 ug/L.
                                       17

-------
     0.8
     0.6
  a
  a
     0.4
     0.2
                Absorbance (WAL Standards)
                Absorbance (5.97 og/L QA/QC Standard)
 Equation for Arsenic Calibration Curve
y = 0.0048851 + 0.058708x R= 0.99985
                    2          4          6          8
                               Arsenic Concentration (ug/L)

                   Figure 2-6.  Typical Arsenic Calibration Curve.
                                             10
12
2.8    Analytical Methods
Table 2-4 shows the various analytical
methods used during the course of this
project.   Only  chlorine  dioxide  was
                        analyzed by multiple methods for  the
                        reasons mentioned  in the Results and
                        Discussion section of this report.
                          Table 2-4. Analytical Methods
Total Arsenic
Chlorine, nitrate, sulfate
Total Organic Carbon
Sulfide

FeandMn
Chlorine (hypochlorite)
Chlorine Dioxide
Ozone
Permanganate
Monochloramine
              SM 3114 A (Hydride Generation)
              SM 4110 B (Ion Chromatography)
              SM 5310 B (Combustion-Infrared)
              Hach Manual. EPA-approved and adapted from
              SM 4500-S2' E (Colorimetric)
              SM 3111 B (Atomic Absorption)
              Potentiometric Method of Knocke, et al. (1990)
              Potentiometric Method of Knocke, et al. (1990)
              SM 4500-C1O2- D (DPD Method)
              Hach Method 8138 (Direct UV  absorbance @445
              nm)
              Hach Method 8311 (Colorimetric)
              Potentiometric Method of Knocke, et al. (1990)
              SM 4500-C1 G (DPD Method)
                                      18

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                         3. Results and Discussion
3.1    Reactor     Set-up     Test
Results
3.1.1  Arsenic(V) Retention
The  IX  filter  was  challenged  with
synthetic  water  (composition shown in
Table      1)     containing     As(V)
concentrations  of 50  and  1000  \ig/L.
The results are shown in Figures 3-1 and
3-2.  It can been seen that for the first
       100  mL  passing  through  the  filter,
       greater than 98% As(V) retention was
       obtained  with  50 ug/L  solution and
       greater than 95%  for the  1000 jag/L
       solution.  For both As(V) concentrations,
       at least 100 mL of synthetic water could
       be put through the  IX filter (@ 20
       mL/min)    before    arsenic   leakage
       exceeded 5%.
               Fresh IX Filter
               First Regeneration
          —V— Second Regeneration
               Third Regeneration
               Fourth Regeneration
               Fifth Regeneration
As(V) = 50 ug/L
Sulfate = 24 mg/L
TDS = 351mg/L
                                                      95% As(V) Retention
               98% Retention
                     50            100            150           200
                         mL passed through IX Filter @ 20 mL/min

Figure 3-1. 50 |ig/L-As(V) Retention by IX Filter.
                                       250
                                       19

-------
  i
  I

  1
     800
     600
400  -
  u
  a 200
  5
       0
            Fresh IX Filter
            First Regeneration
            Second Regeneration
            Third Regeneration
            Fourth Regeneration
            Fifth Regeneration
                                         As(V) = 1000 ug/L
                                         Sulfate = 24mg/L
                                         TDS = 351mg/L
                                                         95% As(V) Retention
    0
                       50            100           150

                           mL passed through IX Filter @ 20 mL/min
Figure 3-2.  1000 ug/L-As(V) Retention by IX Filter.
                                                            200
250
5.1.2  Arsenic(III) Recovery
It was also necessary to demonstrate that
the unoxidized As(III) that passed  from
the   oxidation   reactor   through   the
speciation media was not adsorbed  or
oxidized   by  the   speciation   media.
Figures 3-3  and  3-4 show the  As(III)
recoveries    with     initial     arsenic
                                         concentrations of 50 and 1000 jag/L. No
                                         significant  As(III)  oxidation  resulted
                                         from use of the IX  filter, and As(III)
                                         recovery averaged  95-105% for the 50
                                         fig As(III)/L-spiked water and 90-110%
                                         for the 1000 |J.g As(III)/L-spiked water.
                                         20

-------
   §
   a
   u
     60
     50
     40
     30
     20
     10
      0
Fresh IX Filter
First Regeneration
Second Regeneration
Third Regeneration
Fourth Regeneration
                                                    100% As(III) Recovery
As(m) = 50 jig/L
Sulfate = 24 mg/L
TDS = 351 mg/L
         0                50                100               150

                          mL passed through IX Filter @ 20 mL/min
Figure 3-3.  50 |j.g/L-As(III) Recovery from IX Filter.
                                                               200

^•^
ncentration (fi
Arsenic Cc

1200
1000
son
600
400
200
1
(
; -^--i^^-^^ :
- ^4*^^_V^C^?S=ri^^^^ 100% As(HI) Recovery
—e— Fresh IX Filter
— 0 — First Regeneration As(m) = 1000 fig/L |
— V — Second Regeneration Sulfate = 24 mg/L
— B — Third Regeneration TDS = 351 mg/L
— >? — Fourth Regeneration '
. , , , i , , . , i . . . i i i 	 1 i 	 1 	
) 50 100 ISO 20
                           mL passed through IX Filter @ 20 mL/min
Figure 3-4.  1000 ng/L-As(III) Recovery from IX Filter.
3.1.3  Dead Volume in the Oxidation
Reactor Set-up
The   results   of   the   dead-volume
experiment are presented  in Figure 3-5,
which  shows the  volume  of system
                             effluent that must pass through the IX
                             filter before the actual reactor effluent is
                             sampled. The response was 50 and 80%
                             after 2 and 4 mL of flow, respectively.
                             The true dead volume is probably closer
                                         21

-------
to 2 mL, the point at which 50% of the
response is achieved.  However, due to
the presence  of water in the dead space
above the IX filter hi addition to water
within the filter itself, and the inevitable
mixing   (and  dilution)   that  results
between the incoming flow  from the
reactor and the water in the dead space,
it took 4 mL of flow for the response to
reach 80%  and nearly 8 mL to reach
100%.
£
1
S
o
y
u
§
!2
     1200
     1000
      800
      600
      400
      200
           	100 % Resppnse_   	  		
                       1st Run
                       2nd Run
                       3rd Run
                                                 Synthetic Test Water
                                                 At t=0, inject As(III) = 1000 ug/L
                        246
                           mL passed through IX Filter @ 5 mL/min

Figure 3-5. Estimation of dead volume in the reactor set-up.
A certain initial volume of effluent from
the  reactor   had  to  be  wasted   to
compensate for the dead volume hi  the
system, after which the actual contents
of  the  reactor   could  be  sampled.
Therefore,  if the dead volume was  not
wasted, then the first few samples would
be substantially diluted by  the fluid hi
the dead volume.  Also note that during
actual oxidation experiments, when fluid
exits the reactor, the oxidation reaction
continues because the oxidant is  still hi
contact with As(III). Therefore, the time
spent to waste the dead volume must be
taken into account.

Based on Figure 3-5, it was  decided that
four  mL   (corresponding   to   >80%
response) of the reactor effluent would
have  to  be wasted before  the actual
reactor   contents  could  be  sampled.
Thus, for a sampling  flow rate of 20
mL/min    during    actual    oxidation
experiments,   the   first  sample  that
represented the dead volume (4 mL) was
collected  during   the  0-12  second
interval.  The next sampling interval was
12-18  sec   (median  time   15  sec).
Consequently, no  reaction time earlier
than 15 sec could be studied.

3.1.4 Mixing Efficiency in Oxidation
Reactor
The results of this experiment  showed
that the  effluent arsenic concentrations
(representing unoxidized As(III)) were
                                        22

-------
usually lower than 0.4 ug/L (Figure 3-6).
The low effluent As(III)  concentrations
confirmed   the  rapidity  of  As(III)
oxidation  by  chlorine   as  previously
reported  by Frank and Clifford (1986)
and also showed the efficiency of mixing
                  in the  reactor.   If  the  reactor had
                  provided inadequate mixing, the initial
                  effluent As(III)  concentrations (for t =
                  15  seconds) would  have been  much
                  higher than was actually observed.
    10
  r
               First Run

              • Second Run

              • Third Run
Synthetic Test Water
Initial As(ffl) = 10 ug/L
At t=0, inject «2 = 2 mg/L

Flow Rate=20 mL/min
4 mL wasted as dead volume
Effluent collected at 6 sec (2 mL) intervals)
                        Time elapsed after oxidant addition (seconds)
Figure 3-6. Mixing efficiency in oxidation reactor.
3.2    Chlorine Test Results
The  results  of the chlorine oxidation
experiments are summarized in Table 3-
1 and discussed below.

3.2.1   Effect ofpH on Chlorine
Chlorine rapidly oxidized As(III) in the
pH  range   of  6.3-8.3   (Table   3-1,
experiments   1-3   and  Figure  3-7).
Oxidation was  slightly slower at pH 6.3
but still complete in 39 seconds. Higher
                  As(III)   concentrations   were    also
                  completely  and  rapidly  oxidized  by
                  chlorine  (Table  3-1,  experiment  11).
                  The  time reported (in Table  3-1  and
                  similar  Tables  elsewhere)  to  >95%
                  oxidation was the  median  time of its
                  sample interval, i.e., if greater than 95%
                  oxidation was observed in  the earliest
                  sampling interval,  12-18  sec,  then  the
                  time was reported as 15 seconds.
                                        23

-------
                          Table 3-1. Free Chlorine Experiments.
#
1
2
3
4
5
6
7
8
9
10
11
As(III)
Cone
50
50
50
50
50
50
50
50
50
50
1000
Chlorine
Cone1
(mg/L)
0.14
0.14
0.14
0.48
0.48
0.48
0.48
0.48
0.48
0.14
2.84
SR2
Cl2/As
3
3
3
10
10
10
10
10
10
3
3
SR3
cy
(As+IR)
NA
NA
NA
1.55
0.27
1.20
0.215
O.ll5
NA
NA
NA
pH
8.3
7.3
6.3
8.3
8.3
8.3
8.3
8.3
8.3
8.3
8.3
Interfering
Reductant
(IR)
None
None
None
Fe (II) (0.3 mg/L)
Fe (II) (2.0 mg/L)
Mn (II) (0.2 mg/L)
S2' (1.0 mg/L)
S2' (2.0 mg/L)
TOC (6.9 mg/L)
Temperature (5 °C)
None
>95%
Oxid4
(sec)
15
15
39
15
15
27
57
57
15
15
15
1—Oxidant dosed as NaOCl, reported as mg/L chlorine
2—Stoichiometric Ratio of Oxidant/As(III)
3—Stoichiometric Ratio of Oxidant/(As(III) + Interfering Reductant)
4—Average of duplicate runs
5—Based on oxidation of sulfide to elemental sulfur
      50
                    pH8.3
                    pH8.3
                    pH7.3
                    pH7.3
                    pH6.3
                    pH6.3
As(ni) = 50 jig/L
C12 = 0.14 mg/L (3 x As(ffl) Stoichiometric)
                               100
     150
                                                     200
                           250
                                                                           300
                                                 350
                                          Time (seconds)
Figure 3-7. Effect of pH on As(III) Oxidation with Free Chlorine.
                                            24

-------
5.2.2   Effect of Dissolved Manganese
andiron on Chlorine
The results of the dissolved manganese
and  iron experiments at pH  8.3  are
shown in Table 3-1, experiments 4,  5
and 6. Only a slight effect of dissolved
manganese  was  observed  on  As(III)
oxidation where  complete oxidation was
observed  in  27  seconds with  Mn(II)
present compared with 15 seconds in the
absence of Mn(II).  Dissolved iron had
no effect on As(III) oxidation at pH 8.3.
      3.2.5  Effect ofSulfide on Chlorine
      The results of the sulfide experiments
      are shown in Table 3-1, experiments 7
      and 8.   Although sulfide  (1.0 and 2.0
      mg/L)  slowed the  oxidation reaction,
      complete oxidation was still obtained in
      less than 1 min (Figure 3-8).  However,
      higher   sulfide   concentrations  may
      further  slow As(III) oxidation  so  that
      reaction tunes much greater than 1  mm
      or   higher  oxidant   doses  become
      necessary to achieve  complete  As(III)
      oxidation.
                                 • pH 8.3; No Sulfide
                                 • pH 8.3; No Sulfide
                                 • Sulfide = 1.0 mg/L
                                 • Sulfide = 1.0 mg/L
                                 • Sulfide = 2.0 mg/L
                                 • Sulfide = 2.0 mg/L
                     As(HI) = 50 jigfL
                     Sulfide = 1.0 and 2.0 mg/L
                     CI  = 0.48 mg/L (10 x As (DO) Stoichiometric)
                                  =*=
                           100
150
200
250
300
350
                                     Time (seconds)
Figure 3-8. Effect of sulfide on As(HI) oxidation with free chlorine.
3.2.4  Effect of TOC and Temperature
on Chlorine
The results of the TOC and temperature
experiments  are shown in Table  3-1;
experiments  9  and 10.   Neither the
presence  of  6.9 mg/L  TOC in  Lake
Houston water nor a low temperature of
5 °C had any significant effect on As(III)
oxidation because the time required for
       complete oxidation for both conditions
       was the same as that required when no
       interfering reductants were present.

       3.3    Permanganate          Test
       Results
       Eleven  experiments   were  conducted
       using permanganate as an oxidant, and a
                                       25

-------
summary of the results is shown in Table
3-2
3.5.1  Effect ofpH on Permanganate
Permanganate rapidly oxidized As(III) in
the  pH  range  of 6.3-8.3 (Table  3-2,
experiments   1-3  and  Figure   3-9).
Oxidation was slightly slower at pH 6.3
but still complete in 33  seconds.  Higher
As(III)    concentrations    were    also
completely  and rapidly  oxidized  by
permanganate  (Table  3-2,  experiment
11).
                        Table 3-2. Permanganate Experiments.
#
1
2
3
4
5
6
7
8
9
10
11
As(III)
Cone
50
50
50
50
50
50
50
50
50
50
1000
Mn04-
Conc
(mg/L)
0.16
0.16
0.16
0.53
0.53
0.53
0.53
0.53
0.53
0.16
3.20
SR1
MnO4V
As
3
3
3
10
10
10
10
10
10
3
3
SR2
MnO47
(As+IR)
NA
NA
NA
1.99
0.36
1.55
0.214
O.ll4
NA
NA
NA
pH
8.3
7.3
6.3
8.3
8.3
8.3
8.3
8.3
8.3
8.3
8.3
Interfering
Reductant
(IR)
None
None
None
Fe (II) (0.3 mg/L)
Fe (II) (2.0 mg/L)
Mn (II) (0.2 mg/L)
S2' (1.0 mg/L)
S2' (2.0 mg/L)
TOC (6.9 mg/L)
Temperature (5 °C)
None
>95%
Oxid3
(sec)
15
15
33
21
15
21
51
51
15
15
15
1—Stoichiometric Ratio of Oxidant/As(III)
2—Stoichiometric Ratio of Oxidant/(As(III) + Interfering Reductant)
3—Average of duplicate runs
4—Based on oxidation of sulfide to elemental sulfur
                                         26

-------
  §
                              pH8.3
                              pH8.3
                              pH7.3
                              pH7.3
                              pH6.3
                              pH6.3
                                     150
   200
250
300
350
                                     Time (seconds)
Figure 3-9. Effect of pH on As(III) oxidation with permanganate.
3.3.2   Effect of Dissolved Manganese
andiron on Permanganate
The results of the dissolved manganese
and iron experiments are shown in Table
3-2, experiments 4,  5 and 6.  Neither
dissolved manganese nor dissolved iron
(Fe(II)) had  any  significant effect  on
As(III)   oxidation  at  pH  8.3   with
complete  oxidation  achieved  in   21
seconds or less.
3.3.3   Effect     of    Sulflde     on
Permanganate
The results  of the  sulfide experiments
are shown in Table 3-2, experiments 7
and 8.  Although sulfide (1.0 and 2.0
mg/L) slowed the  oxidation reaction,
complete oxidation was still obtained in
less than 1 min (Figure 3-10). However,
higher   sulfide  concentrations   may
further slow As(III) oxidation such that
reaction times much greater than  1  min
and/or higher  oxidant doses  will  be
required to achieve complete oxidation.
                                       27

-------
     50
     40
     30
     20  -]
  !  10
As(ffl) = 50 ug/L
Sulfide = 1.0 and 2.0 mg/L
          pH 8.3; No Sulfide
          pH 8.3; No Sulfide
          Sulfide = 1.0 mg/L
          Sulfide = 1.0 mg/L
          Sulfide = 2.0 mg/L
          Sulfide = 2.0 mg/L
              MnO4' = 0.53 mg/L (10 x As(III) Stoichiometric)
                  50
             100
150
200
                                                        250
                                                    300
                                       350
                                    Time (seconds)
Figure 3-10. Effect of sulfide on As(III) oxidation with permanganate.
3.3.4  Effect of TOC and Temperature
on Permanganate
The results of the TOC and temperature
experiments are  shown  in  Table 3-2,
experiments 9 and  10.    Neither the
presence of TOC nor a low temperature
of 5 °C had  any significant effect  on
As(Tfl)   oxidation   by   permanganate.
                             Complete oxidation was  achieved for
                             both the conditions in 15 seconds.

                             3.4    Ozone Test Results
                             Eleven   experiments  were  conducted
                             using  ozone  as  an  oxidant,  and  a
                             summary of the results is shown in Table
                             3-3.
                                       28

-------
                            Table 3-3. Ozone Experiments.
#
1
2
3
4
5
6
7
8
9
10
11
Cone
50
50
50
50
50
50
50
50
50
50
50
Ozone
Cone
(mg/L)
0.10
0.10
0.10
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.10
SR1
3
3
3
10
10
10
10
10
10
10
3
SR2
95%
Oxid3
(sec)
15
15
15
15
15
15
51
132
27
NA5
39
1—Stoichiometric Ratio of Oxidant/As(III)
2—Stoichiometric Ratio of Oxidant/(As(III) + Interfering Reductant)
3—Average of duplicate runs
A—Based on oxidation of sulfide to elemental sulfur
5—Max. oxidation achieved with 6.9 mg/L TOC was 38%
3.4.1  Effect ofpH on Ozone
In the range of 6.3-8.3, pH had no effect
on  As(III)   oxidation  with   complete
oxidation achieved in 15 seconds as seen
in Table 3-3, experiments 1-3 and Figure
3-11.
                                       pH8.3
                                       pH8.3
                                       pH7.3
                                       pH7.3
                                       pH6.3
                                       pH6.3
                      As(ffl) = 50
                      O3 = 0.1 mg/L (3 x As(III) Stoichiometric)
                              100        150        200
                                         Time (seconds)
Figure 3-11.  Effect of pH on As(III) oxidation with ozone.
              250
300
350
                                           29

-------
3.4.2  Effect of Dissolved Manganese
andiron on Ozone
The results of the dissolved manganese
and iron experiments are shown in Table
3-3, experiments 4,  5  and 6.   Neither
dissolved Mn (0.2  mg/L) nor dissolved
Fe (0.3 and 2.0 mg/L) had any effect on
As(III) oxidation by ozone  at pH 8.3,
with complete  As(III) oxidation being
achieved in 15 seconds.
3.4.3  Effect ofSulfide on Ozone
The results  of the  sulfide experiments
are shown in Table 3-3,  experiments 7
and 8.  The presence of  sulfide  at 1.0
       and 2.0 mg/L slowed As(III) oxidation
       by  ozone  at pH  8.3.    At  sulfide
       concentrations of 1.0 and 2.0   mg/L,
       greater than 95% oxidation was achieved
       in 51  and  132  seconds, respectively
       (Figure 3-12).  By  comparison,  in the
       absence of sulfide, >95% oxidation was
       achieved in  just  15  seconds and at a
       much lower ozone dose  of three-times
       the  stoichiometric requirement.   Note,
       however, that in the presence of 1.0 and
       2.0 mg/L sulfide  (SR = 0.21  and 0.11,
       respectively),  there   was  insufficient
       ozone  to oxidize  both  As(III)  and
       sulfide.
                                  pH 8.3; No Sulfide
                                   H 8.3; No Sulfide
                                   ulfide = 1.0 mg/L
                                  Sulfide = 1.0 mg/L
                                  Sulfide = 2.0 mg/L
                                  Sulfide = 2.0 mg/L
                      As(ffl) = 50 ug/L, pH = 8.3
                      Sulfide = 1.0 and 2.0 mg/L
                      O = 0.32 mg/L (10 x As(IH) Stoichiometric)
                          100
150
200
250
300
350
                                     Time (seconds)
Figure 3-12. Effect of sulfide on As(III) oxidation with ozone.
3.4.4  Effect of TOC and Temperature
on Ozone
The results of the TOC and temperature
experiments are shown  in  Table 3-3,
experiments 9 and 10.  The presence of
TOC had a significant quenching effect
       on the ability of ozone to oxidize As(III)
       in filtered Lake Houston water (Figure
       3-13).  With 6.9 mg/L TOC, there was
       insufficient  ozone present to  oxidize
       both As(III) and the TOC.  Since most
       arsenic-contaminated  groundwaters  are
                                       30

-------
unlikely to contain such high TOC, the
Lake  Houston  water was  diluted with
synthetic water (composition shown in
Table 2-1) to yield a TOC of about 2.1
mg/L.  In the presence of this lower TOC
concentration,  ozone  was  able  to
efficiently oxidize As(III), with greater
than  95%  oxidation  achieved in  27
seconds.
                      Initial As(III) = 50 pg/L
                      pH = 8.3
                      O, = 0.32 mg/L (10 x As(ni) Stoichiometric)
                        3
                                      TOC = 6.9 mg/L
                                      TOC = 6.9 mg/L
                                      TOC = 6.9 mg/L
                                      TOC = 2.1 mg/L
                                      TOC = 2.1 mg/L
                                      TOC = 2.1 mg/L
                                      TOC = 2.1 mg/L
                                               200
             250
300
350
                                     Time (seconds)
Figure 3-13.  Effect of TOC on As(III) oxidation with ozone.
3.5    Chlorine    Dioxide    Test
Results
Thirteen arsenic oxidation  experiments
with  chlorine dioxide were performed
assuming  a conservative  one-electron
transfer   mechanism,   i.e.,   chlorine
dioxide  is  converted to C1O2"  while
oxidizing As(III) to As(V).  The five-
electron transfer mechanism  was only
used  in  calculating  chlorine  dioxide
doses   for    the    Fe(H)-oxidation
experiments (Knocke, et al., 1990). The
results   of  the   thirteen  tests   are
summarized in Table 3-4.
3.5.1  Effect  of  pH  on  Chlorine
Dioxide
In  the pH  range of  6.3-8.3,  chlorine
dioxide produced limited oxidation (20-
30%) in 21  seconds and  produced no
further     oxidation    (Table     3-4,
experiments 1, 2, 3 and 9). In order to
verify that the IX filter was undamaged
by  chlorine  dioxide  and  functioning
normally, two more  experiments were
performed, which included a three-times
Stoichiometric   amount   of   chlorine
injected  at  117  seconds.   If filter
integrity was maintained, the unoxidized
As(HI)  concentration would be  hi the
range of  35-40  ug/L  after  chlorine
dioxide addition, and then quickly drop
to zero due to complete As(III) oxidation
                                        31

-------
range   of  35-40   |J.g/L  after  chlorine
dioxide addition, and then quickly drop
to zero due to complete As(in) oxidation
after the addition  of  chlorine.    The
results of these experiments are shown in
Figure  3-14,  which shows  that only  20-
30%  oxidation   was  obtained  with
chlorine dioxide, but complete oxidation
was  achieved  after  the  addition  of
chlorine. This test confirmed that the IX
filter,   which  separates  As(ni)  from
As(V), was still effective after exposure
to chlorine dioxide.
                        Table 3-4. Chlorine Dioxide Experiments.
# As(m) C1O2 Cone
Cone (|ag/L) (mg/L)
1
2
3
4
5
6
7

8
9
10

11

12
13
50
50
50
50
50
50
0

0
505
50

50

50
50
0.27
0.27
0.27
0.90
0.90
0.90
1.08

1.08
0.27
C1O2=0.27
C12=0.14
0.27

0.90
9.00
SR1 SR2
cio2/As cicy
(As+m.)
3
3
3
10
10
10
NA

NA
3
3
3
3

10
100
NA
NA
NA
5.56
1.58

1.12

1.12
NA
NA

NA

NA
NA
pH
8.3.,
7.3
6.3
8.3
8.3
8.3
8.3

8.3
8.3
8.3

8.3

8.3
8.3
Interfering
Reductant
OR)
None
None
None
Fe (H) (0.3 mg/L)
Fe (H) (2.0 mg/L)
Mn (H) (0.2 mg/L)
Fe(H) (4.0 mg/L)4
Without silica
Fe(D[) (4.0 mg/L)4
None
None

Deaerated
synthetic water
None
None
>95%
Oxid3
(sec)
>323
>312
>312
>312
>312
>312
15

15
>312
>NA6

>312

>312
>312
1—Stoichiometric Ratio of Oxidant/As(III)
2—Stoichiometric Ratio of Oxidant/(As(III)  + Interfering Reductant) based on a 1-electron transfer
mechanism, except for Fe(II) which is oxidized by chlorine dioxide by a 5-electron transfer mechanism
3—Average of duplicate runs
4—Experiments 7 and 8 were performed to duplicate the Fe(II) oxidation experiments of Knocke (1990).
Silica was eliminated from #7 as it is known to complex Fe.
5—Repeat of experiment # 1
6—Complete Oxidation was achieved only after the addition of chlorine
                                           32

-------
    50
  a
     20
     10
      0
            As(UT) = 50 ug/L, pH = 8.3
            CIO2 = 0.27 mg/L (3 x As(m) Stoichiometric)
Cl = 0.14 mg/L (3 x) injected at t
                                          117 sec
1A-CIO2 only
1B-C1O2 only
2A-C1O2 only
2B-C1O2 only
3A-C1O2, then
3B-CIO2, then
        0
     50
                           100
                                                        250
                                                      300
                   350
                                     150        200

                                     Time (seconds)

Figure 3-14. Arsenic(III) oxidation with chlorine dioxide.  Chlorine injected at 117 sec to
verify IX filter performance in experiments 3A and 3B.
The ineffectiveness of chlorine dioxide
was surprising as it is known to be a
powerful   oxidant.     Moreover,   the
phenomenon of 20-30% oxidation in 21
seconds with no further oxidation could
not be explained.  The scope of research,
the   budget,   and   time   constraints
preempted further investigation into the
reasons    for    this   ineffectiveness.
Possibly,  the  initial  As(III)  oxidation
seen in the chlorine dioxide experiments
was due to the presence  of chlorine in
the  chlorine  dioxide  stock  solutions.
Knocke  et  al.  (1990)  reported  the
formation of about 20 mg/L HOC1 (27
mg/L as chlorine) along with 750 mg/L
C1O2 (390  mg/L as chlorine).   It is
possible that the rapid  initial  As(III)
oxidation observed in these experiments
was the result of the presence of a very
small fraction  of chlorine in the C1O2
stock  solutions,  and  was not  due to
chlorine dioxide itself. It should also be
noted that  this  fraction of chlorine
                                probably  varied  from  one  batch to
                                another.   Therefore, an increase  in the
                                concentration of chlorine dioxide dose
                                does   not    necessarily   imply   a
                                proportionate increase in  the chlorine
                                fraction as well.

                                3.5.2   Verifying    Chlorine   Dioxide
                                Stock Concentrations.
                                In order to verify the ineffectiveness of
                                chlorine    dioxide    and   rule   out
                                measurement errors, the chlorine dioxide
                                stock solution was assayed to ensure that
                                the proper oxidant  dose  was  delivered.
                                Three different methods  were used: (1)
                                Potentiometric method from Knocke et
                                al.  (1990), (2)  Standard  Method 4500-
                                C1O2-D (DPD  Method),  and  (3)  Hach
                                Method 8138,  a direct-reading method
                                with a range of 0-700 mg/L.   Both the
                                Potentiometric  and DPD methods gave
                                excellent  agreement while  the  Hach
                                method  underestimated   the  chlorine
                                        33

-------
dioxide concentration by about 6%.  The
results are shown in Table 3-5.
                        Table 3-5. Chlorine Dioxide Assays.
Method

SM4500-C1O2-D
Potentiometric
Hach8318
Observed
Value (mg/L)
458
453
430
Difference1
%
-
-1.1
-6.1
               1—Difference from value obtained using the Standard Method assay.
3.5.3  Fe(II)  Oxidation with Chlorine
Dioxide
Knocke et al. (1990) reported essentially
instantaneous  oxidation  of Fe(II)  to
Fe(III) by chlorine dioxide at a dose of
105%  of  stoichiometric  requirement.
These experiments were repeated for an
initial Fe(II) concentration  of 4  mg/L.
The results of  these  experiments are
shown in Table  3-4, experiments 7 and
8. With a chlorine dioxide dose of 1.08
mg/L (1.12  tunes   stoichiometric),  an
initial Fe(II) concentration  of 4 mg/L
was completely oxidized in  15 seconds.
To   eliminate  dissolved  oxygen  as  a
potential cause for the ineffectiveness of
chlorine  dioxide for As(III) oxidation,
tests were  performed  in low-DO (0.1
mg/L) synthetic  water.  The absence of
DO did not improve the performance of
chlorine dioxide for As(III) oxidation.

3.5.4 Increasing Stoichiometric Dose
of Chlorine Dioxide
The chlorine dioxide dose was increased
to  ten-  and  one-hundred-times  the
stoichiometric   amount   required  for
As(III)  oxidation.   These  results are
shown in Table 3-4,  experiments 12 and
13.   Even at a chlorine dioxide dose of
9.0  mg/L (100  x stoichiometric), only
about  76%   As(III)  oxidation  was
observed at the end of 5 minutes.

3.5.5  Effect  of Dissolved Manganese
andiron on Chlorine Dioxide
The  results of the  dissolved manganese
and iron experiments are shown in Table
3-4,  experiments  4,  5  and  6.   The
presence  of dissolved manganese and
iron  in the synthetic water resulted in
somewhat lower As(III) concentration in
the  reactor  effluent  compared  with
synthetic   water  without   interfering
reductants present.  The  lower residual
As(III)  concentrations observed were
due to adsorption of As(III) and As(V)
onto the  manganese and iron hydroxide
precipitates that formed  upon chlorine
dioxide addition.

3.5.6  Effect  of  Sulflde,  TOC and
Temperature on Chlorine Dioxide
Because   of   the   ineffectiveness   of
chlorine  dioxide as  an oxidant in the
absence of interfering reductants, As(III)
these   experiments   were   deemed
unnecessary.
                                       34

-------
3.6    Monochloramine
Results
                 Test       F°ur experiments  were performed with
                             monochloramine as oxidant.  The results
                             are summarized in Table 3-6.
                      Table 3-6. Monochloramine Experiments.
#


1
2
3
4
AsCffl)
Cone
(l^g/L)
50
50
50
50
NH2C1
Cone
(mg/L)
0.10
0.10
0.10
0.104
SR1
NH2C1/
As
3
3
3
3
SR2
NH2C]7
(As+IR)
NA
NA
NA
NA
pH


8.3
7.3
6.3
8.3
Interfering
Reductant
(IR)
None
None
None
None
>95%
Oxid3
(sec)
>312
>312
>312
>312
       1—Stoichiometric Ratio of Oxidant/As(III)
       2—Stoichiometric Ratio of Oxidant/(As(III) + Interfering Reductant)
       3—Average of duplicate runs
       4—With preformed monochloramine
3.6.1  Effect      of     pH       on
Monochloramine
In  the pH  range  of  6.3-8.3,  limited
oxidation  (40%)  was  produced  by  in-
                             szYw-formed monochloramine in the first
                             21 seconds with no oxidation observed
                             thereafter.
  •i
  •s
  1
  8
      50
      40
      30
      20
      10
       0
                As(III)=50ng/L
                NILCI = 0.1 mg/L (3 x As Stoichiometric)
pH 8.3; in-situ-formed Monochloramine
pH 8.3; in-situ-formed Monochloramine
pH 7.3; in-situ-formed Monochloramine
pH 7.3; in-situ-formed Monochloramine
pH 6.3; in-situ-formed Monochloramine
pH 6.3; in-situ-formed Monochloramine
pH 8.3; preformed Monochloramine
pH 8.3; preformed Monochloramine
         0
  50
100
150
200
                                       Time (seconds)
Figure 3-15. Arsenic(m) oxidation with monochloramine.
250
300
350
                                          35

-------
These results are in agreement with the
findings  of Frank and Clifford  (1986)
who speculated  that the occurrence of
rapid initial As(III) oxidation was due to
free chlorine before  it  reacted  with
ammonia to form monochloramine. To
test  this  hypothesis,   a  10   mg/L
monochloramine solution was prepared,
assayed,  and used as a stock solution.
When preformed monochloramuie was
added to  the reactor, no As(III) oxidation
was observed (Figure 3-15). Because a
three-times-stoichiometric   amount  of
monochloramine failed to produce any
As(III)  oxidation, it was not studied
further.

3.7  Solid-Phase       Oxidizing
Media (Filox)
Twenty-four     experiments      were
conducted with the Filox media and the
test results are summarized in Table 3-7.
The variables studied for their effect on
As(III) oxidation included  EBCT, pH,
DO, dissolved Mn, dissolved Fe, sulfide,
TOC  and initial  As(III) concentration.
All of the Filox experiments described in
this report were performed with the same
12 mL of Filox Media.   The media was
backwashed after each experiment, but
was otherwise untreated.

3.7.1   Filox:   Effect of  Empty  Bed
Contact Time with High-DO
The effect of EBCT, from 0.75 to 6 min,
on As(III) oxidation is  shown in Figure
3-16  and Table 3-7,  experiments 1-4.
Even  at the shortest EBCT  of 0.75 min,
greater than 95% As(III) oxidation was
obtained.   The  media also removed
arsenic, in addition to oxidizing it,  as
seen in Figure 3-16.   Arsenic removal
increased from 45 to 64% as  the EBCT
increased from 0.75 to 6.0 min.
                                       36

-------
                           Table 3-7. Filox Experiments.
#

1
2
3
4

5
6

7
8
9
10
11
12
13
14

15
16
17
18
As(III)
Hg/L
Interfering
Reductant
(IR, mg/L)
pH
DO1
(mg/L)
EBCT2
(min)
% As(III)
Oxidation
%As
Removal
Variable EBCT
50
50
50
50
None
None
None
None
8.3
8.3
8.3
8.3
8.2
8.2
8.2
8.2
0.75
1.5
3.0
6.0
95.4
98.7
98.7
99.4
45.3
48.2
55.0
63.6
Variable pH
50
50
None
None
7.3
6.3
8.2
8.2
1.5
1.5
99.8
100.0
66.2
75.3
Interfering Reductants, Low-DO, Low EBCT
50
50
50
50
50
50
50
50
None
Mn(II)— 0.2
Fe(II)— 0.3
Fe(II)— 2.0
Sulfide— 1.0
Sulfide— 2.0
TOC— 1.4
None/8 °C
8.3
8.3
8.3
8.3
8.3
8.3
8.3
8.3
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
1.5
1.5
1.5
1.5
1.5
1.5
1.5
1.5
98.9
78.2
80.9
81.3
61.5
55.7
79.2
98.3
36.1
12.4
19.1
13.9
5.0
9.4
7.2
36.5
Interfering Reductants, Low-DO, High EBCT
50
50
50
50
Mn(II)— 0.2
Sulfide— 1.0
Sulfide— 2.0
TOC— 1.4
8.3
8.3
8.3
8.3
0.1
0.1
0.1
0.1
6.0
6.0
6.0
6.0
99.8
99.6
97.0
99.9
51.1
57.4
42.4
47.5
  19
  20
High Initial Arsenic(III) Concentration
  1000   I None            I  8.3  I   0.1
  1000   I None            |  8.3  |   8.2

Interfering Reductants, High-DO, Low EBCT
1.5
1.5
96.7
95.0
1—High DO = 8.2 mg/L; Low DO = 0.1 mg/L
2—EBCT of 0.75, 1.5, 3.0 and 6.0 equal 4.9,2.5,1.2 and 0.6 gpm/ft2, respectively.
44.8
50.3
21
22
23
24
50
50
50
50
Mn(II)— 0.2
Sulfide— 1.0
Sulfide— 2.0
TOC— 1.4
8.3
8.3
8.3
8.3
8.2
8.2
8.2
8.2
1.5
1.5
1.5
1.5
98.4
98.3
96.2
97.5
50.8
76.1
77.1
55.4
                                         37

-------
 I
 3
  a
 •2
 £
 a
  I
 1
     50
    40
    30
    20
     10
               • As(III) Remaining  I
               • Total Effluent As   |
Initial As(III) = 50
pH = 8.3
DO = 8.2 mg/L
                                         Line of 90% Arsenic(III) Oxidation
         0
                         1.5                3
                             Empty Bed Contact Time (min)

Figure 3-16. Effect of EBCT on Filox Media.
                                                  4.5
In  an  effort   to   stabilize  arsenic
adsorption,  attempts  were   made   to
equilibrate the Filox media with As(III)-
spiked synthetic water before any further
experiments were performed.   Hence,
2000  BV (24  L) of 50  ug/L As(III)-
spiked  synthetic water   was  passed
through the Filox media at a flow rate of
16 mL/min corresponding to  an EBCT
of 0.75 min and a total equilibration time
of 25 hours of interrupted flow over a
three-day period.  The results are shown
in Figure  3-17.  Arsenic  oxidation rate
started  out at 96% (2  ug/L  As(III)
remaining)  and  decreased slightly  to
93% (4 ug/L As(III) remaining) at 2000
BV.   Arsenic removal  by the  Filox
                                            media decreased significantly as the run
                                            progressed.  From an initial removal rate
                                            of 26%, arsenic removal decreased to
                                            8%  at  2000  BV,  indicating  that the
                                            media's capacity for arsenic adsorption
                                            was  nearly exhausted.  Figure 3-17 also
                                            shows that the Filox media will initially
                                            oxidize  As(III) and remove it, but will
                                            eventually come to equilibrium with the
                                            influent arsenic and provide no further
                                            arsenic  removal  while  still  oxidizing
                                            As(III) to As(V). It should also be noted
                                            that  no  arsenic was dumped from the
                                            Filox   media  in   the   equilibration
                                            experiment   or   in  any  subsequent
                                            experiments.
                                        38

-------
  I
     50
     40
     30
  a  20
  S  10
      0
  •  Toi
  O  Asi
                        uent As
                       .emaming
Initial As(III) = 50 fig/L
pH = 8.3
DO = 8.2 mg/L
                                         Line of 90% Arsenic(III) Oxidation
             O  o
                   o°o0ooooo0oo0o°°o!>
         0
              500
1500
2000
                                          1000
                                      Bed Volumes
Figure 3-17.  Filox media equilibration with 2000 BV of synthetic test water at an EBCT
of 0.75 min.
3.7.2  Filox: Effect of pH with High-
DO
The  results on the pH tests in the range
of 6.3-8.3  showed  that  pH  had no
significant effect on arsenic oxidation by
the Filox media (Figure 3-18).  Greater
than 98% As(III) oxidation was achieved
in the pH range of 6.3-8.3.  Total arsenic
(As(III)  +  As(V))  removal, however,
increased with  decreasing pH,  with
removals increasing from 48% at pH 8.3
to 75% at pH 6.3. It should be noted that
in Figure 3-18, arsenic removal  by the
Filox media was much higher than at the
                                 end  of   the  previously  performed
                                 equilibration  experiments.   This was
                                 probably  due to a  relaxation  in the
                                 concentration gradient that occurs when
                                 flow through adsorbent-packed media
                                 bed is  interrupted and then  resumed.
                                 Morevover, the longer contact time of
                                 1.5  min used in the  post-equilibration
                                 experiments,  compared with  the  0.75
                                 min   contact  time   used   in   the
                                 equilibration   experiments,  will  also
                                 result in increased arsenic adsorption by
                                 the Filox media as seen in Figure 3-16.
                                        39

-------
  i
   8
   a
   o
  U
   g
     50
     40
     30
     20
     10
                • As(III) Remaining
                • Total Effluent As
           Initial As(HI) = 50 fig/L
           EBCT=1.5min
           DO = 8.2 mg/L
                                         Line of 90% Arsenic(III) Oxidation
                                              PH
Figure 3-18. Effect of pH on As(III) oxidation with Filox media.
3.7.3  Filox:   Effect  of DO  in the
absence of interfering reductants
In the absence of interfering reductants,
the concentration of dissolved oxygen in
the  synthetic water had  no effect on
As(III) oxidation by the  Filox  media
(Table 3-7,  experiments 1-7).   Similar
results were  obtained  for the  higher
initial As(HI) concentration  of  1000
ug/L (Table  3-7, experiments  19 and
20).  This lack of effect is supported by
the studies of Scott and Morgan (1995)
who concluded that DO had no effect on
As(IH) oxidation by 8-MnO2.
               Effect  of  Interfering

                             Synthetic
3.7.4  Filox:
Reductants
3.7.4.1 Low-DO  (0.1  mg/L)
Water at 1.5 min EBCT
All of the  interfering reductants studied
had   an  adverse  effect   on  As(III)
oxidation in  low-DO synthetic water at
pH 8.3 and EBCT of 1.5 min (Table 3-7,
experiments  8-13).    Sulfide  had  the
greatest  effect  on  As(III) oxidation,
which   decreased from 99% with  no
sulfide present to 62 and 56% at sulfide
levels of 1.0 and 2.0 mg/L, respectively.
Similarly, in the  presence  of 1.4 mg/L
TOC, As(III) oxidation was reduced to
79% compared with 99% when no TOC
was present.

3.7.4.2Low-DO   (0.1  mg/L)  Synthetic
Water at 6.0 min EBCT
To  attenuate the  effect of  interfering
reductants in low-DO water,  the EBCT
was increased from 1.5 to  6.0 min.  At
an EBCT of 6.0 min, the effects of all of
the interfering  reductants  on  As(III)
oxidation were  completely  attenuated
(Table  3-7,  experiments  15-18),  and
As(III)  oxidation results matched those
when  no  interfering  reductants  were
present.

3.7.4.3High-DO  (8.2  mg/L)  Synthetic
Water at 1.5 min EBCT
Near-complete  arsenic  oxidation  was
also  obtained   in  the   presence  of
                                        40

-------
interfering reductants when the synthetic
test water contained sufficient dissolved
oxygen  as  seen   from  Table   3-7,
experiments 21-24.  When 8.2 mg/L DO
was  present,  As(III) oxidation results
matched  those  when   no  interfering
reductants were present.

5.7.5  Fttox:      Effect   of   Low
Temperature   in  low-DO   Synthetic
Water
Lowering  the  temperature  of  the
synthetic water from 24 °C (approximate
room temperature) to 8 °C had no effect
on arsenic oxidation  with an oxidation
efficiency  of 98.3%  achieved  at  8°C
compared with 99% at 24 °C (Table 3-7,
experiments 7 and 14).

3.8   UV Oxidation Results
Two   UV   units    from   different
manufacturers  were  tested  for  their
ability  to  oxidize  As(III).    Fifteen
experiments were performed using UV
to oxidize As(III) and the results  are
summarized      in     Table     3-8.
Table 3-8. UV Oxidation Experiments.
#

UVDose Flow Rate Contact pH %As(III)
W-sec/cm2
UVUnitl: 32,000 ftw/c
1
2
3
4
5
6
7
8
UV
9
10
11
12
13
14
15
1.9
5.8
11.5
23.0
46.1
46.1
46.1
1.8a
mL/min Time (min)
:m? at a
288
96
48
24
12
12
12
310
Unit 2: 41, 200 juw/cm2 at a
3.9
5.8
11.5
23.0
46.1
46.1
46.1
310
209
105
52
26
26
26
design flow rate of 1890 mL/min
1 8.3
3 8.3
6 8.3
12 8.3
24 8.3
24 7.3
24 6.3
0.9 8.3
design flow rate of 3785 mL/min
1.6 8.3
2.3 8.3
4.7 8.3
9.3 8.3
18.7 8.3
18.7 7.3
18.7 6.3
Oxidation

4
14
27
58
73
71
64
100

2
5
12
29
43
40
27
   a—Spiked with 1.0 mg/L sulfite, SO3", to verify reported catalytic effect of sulfite on UV
   oxidation of As(III).
3.8.1   Unitl
Unit  1  (R-Can  Environmental  Inc.,
Canada)  had a design flow rate of 0.5
gpm (1890 mL/min) and provided a UV
intensity of 32,000  uW/cm2.  At a flow
rate of 288 mL/min (contact time of 1
                                       41

-------
min), i.e., about 15% of the rated flow,
no  significant  oxidation was observed
(Table  3-8,  experiments  1-7).     A
maximum of 73% As(III) oxidation was
obtained at 12 mL/min flow rate (contact
time of 24 min), i.e., 0.6% of the rated
flow.  This translates to an  extremely
high  energy input of 46,080,000  uw-
sec/cm2, whereas  only    6,500  uw-
sec/cm2 is required to achieve a 99.9%
destruction level ofE.Coli.

To  verify the reported catalytic effect of
sulfite on As(III) oxidation by UV (MSB
Technology Applications, Inc., Montana
and ANSTO, Australia (1997; Khoe et
al.  (1997) and  Khoe et al. (2000)), the
synthetic  water  was  spiked  with  1.0
mg/L of sulfite and allowed to stand for
30 minutes. The sulfite-spiked synthetic
water  was then passed through Unit  1
where complete oxidation of As(III) was
obtained, whereas virtually no oxidation
occurred in the unspiked synthetic water
under the same conditions (Table 3-8,
experiment  8).  Thus,  sulfite played  a
role  in facilitating  the oxidation  of
As(III).   The  mechanism of  sulfite-
facilitated As(TII) oxidation is a matter
of  speculation.    Sulfite  is  not an
oxidizing  agent   and  control   tests
performed with the sulfite-spiked water,
with the UV lamp turned off, showed no
As(III) oxidation.   It  is possible that
sulfite catalyzed the formation of free
radicals  and  thus  assisted  hi   the
oxidation of As(III). The role of sulfite
in facilitating As(III)  oxidation  merits
further research.

3.8.2   Unit 2
Qualitatively,    Unit    2    (Atlantic
Ultraviolet Corp.) with  a design flow
rate  of  1  gpm  (3,785  mL/min) gave
results similar to those  obtained using
Unit  1  (Table 3-8,  experiments  9-15).
However, for the same UV doses,  Unit 2
produced less oxidation than Unit  1.
The   reasons for  the  difference  in
performance between the two UV units
was not studied.

It should be noted that the off-the-shelf
UV units studied were designed for the
primary  purpose of disinfection and,
therefore, were not optimized for As(III)
oxidation.    Arsenic  shows  a  strong
absorbance at 193.7 nm, which  is the
wavelength used in  AA units for arsenic
analysis. Therefore, a more appropriate
wavelength to  study UV-oxidation of
As(III) would be near 193.7  nm.  UV
systems, with peak  intensity at 185 nm,
are commercially available and may be
more appropriate for such a study.

It  should  also  be  noted  that  the
difference     in      arsenic-oxidation
performance  of the two  UV units does
not  imply  that  they would perform
differently   for   a   disinfection-type
application.
                                        42

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                      4. Summary and Conclusions
Bench-scale studies were performed to
assess the feasibility of using three- and
ten-times stoichiometric amounts (based
on   As(m)   concentration)   of   five
chemical oxidants, a solid-phase oxidant,
and UV radiation for As(IE) oxidation to
As(V).    The  effects   of   interfering
reductants      including     dissolved
manganese (0.2 g/L), dissolved iron (0.3
and 2.0 mg/), sulfide (1.0 and 2.0 mg/L)
and  TOC (2.1 and  6.9  mg/L)  on the
effectiveness  of these  oxidants   was
examined.  The conclusions  of the one-
year lab  study  on  As(in) oxidation
performed at the University  of Houston
are summarized below.

(1) Chlorine rapidly oxidized As(HT) to
As(V)  under all  the  conditions tested.
Iron and manganese had no  measurable
effect on  As(HI) oxidation.   Although
sulfide   and   TOC   slowed   As(in)
oxidation    by   chlorine,   complete
oxidation was still obtained in less than
one minute.  Lowering the temperature
from 25 to  5 °C  had no  measurable
effect on As(HI) oxidation in  the absence
of interfering reductants.

(2) Permanganate also rapidly oxidized
As(in) to As(V). Even in the presence
of interfering reductants, greater  than
95% As(m) oxidation was achieved by
permanganate  in   <   51   seconds.
Lowering the temperature from 25 to 5 °
C had  no measurable effect on As(H[)
oxidation  in the absence  of interfering
reductants.

(3)   In  the  absence  of   interfering
reductants,   ozone   rapidly  oxidized
As(ffl) in the pH range of 6.3-8.3.  No
adverse  effect  was  observed  in  the
presence of either dissolved manganese
or dissolved iron.  Although  complete
oxidation was  obtained,  the  rate  of
As(m)   oxidation   was   considerably
slower in the presence of sulfide.

TOC had the greatest adverse effect on
As(m)  oxidation  by  ozone.    In  the
presence of 6.9 mg/L TOC in As(m)-
spiked Lake Houston water,  only 34%
As(ni)  oxidation was produced  in  21
seconds. Thus, the presence of TOC had
a quenching effect on ozone.  When the
TOC in the Lake Houston was reduced
by dilution with synthetic test water to
2.1  mg/L, complete oxidation of As(ni)
was observed in 27 seconds.  Lowering
the  temperature from 25 to 5 °C had no
measurable effect on As(HT) oxidation in
the absence of interfering reductants.

(4)  Chlorine dioxide was, surprisingly,
ineffective for  As(in)  oxidation.   A
three-fold   stoichiometric   dose   of
chlorine dioxide produced only 20-30%
oxidation in 21  seconds and produced no
additional oxidation thereafter.  Even a
100-times stoichiometric dose  produced
only 76% oxidation in 5 minutes. The
limited oxidation that was observed was
probably due to the presence of chlorine
as a contaminant in the chlorine dioxide
stock solutions as  chlorine is known to
be a by-product of the chlorine dioxide
generation process.

(5)  Monochloramine was ineffective as
an  oxidant  for  As(m)  confirming the
findings of other researchers.    While
                                       43

-------
limited As(III) oxidation resulted when
monochloramine was formed in-situ, no
oxidation was observed when preformed
monochloramine  was  used.      This
suggests that when chlorine is dosed into
an  As(III)-containing solution  in the
presence of excess  ammonia, a fraction
of that chlorine reacts with As(III) before
it is completely quenched by ammonia to
form monochloramine.

(6)  Filox media was found to be very
effective  for  As(III) oxidation  under
most  of the conditions tested.  In the
absence of interfering reductants, greater
than 95% As(III) oxidation was achieved
in both  low-and  high-DO waters  at
contact times as short as  1.5 min (15
gpm/ft2 hi a 3-ft deep bed).  In addition
to As(III) oxidation, the Filox media also
removed arsenic by adsorption onto the
media, but the adsorption decreased as
the media came into equilibrium with the
feed water.

   As(III)  oxidation  by  Filox  was
adversely affected hi the presence of all
of the interfering reductants  tested in
low-DO water at an EBCT of 1.5  min
(2.4 gpm/ft2) with sulfide exhibiting the
greatest effect. The effects of interfering
reductants  were  completely  attenuated
by either increasing the contact time to 6
min  or by increasing the DO to 8.2
mg/L.  As(III) concentrations as high as
1000 |o.g/L were efficiently oxidized (>
95%) in both low- and high-DO waters
at pH  8.3 in the absence  of interfering
reductants.

(7) UV radiation alone was ineffective
for As(III)  oxidation unless extremely
high UV doses, (7000 times the UV dose
required  for E.Coli  inactivation)  were
used.   Even with such a high UV dose,
only   73%  As(III)  oxidation   was
observed.   However, as described  in a
patented process, the presence of sulfite
provided for the rapid and quantitative
oxidation of As(III).  The mechanism by
which  sulfite promotes the oxidation of
As(III) was not studied here.
                                        44

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                               5. References
Amy, G.  et  al.   Arsenic  Treatability
   Options and Evaluation of Residuals
   Management    Issues     (90771).
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Catherine, H.A.  The Electrochemical
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   Electron Transfer Reaction. Journal
   of Physical Chemistry, Vol. 71, No.
   2, p 268-274 (1967).
Clifford,  D.A. and Lin, C.C.  Arsenic
   Removal  from Drinking  Water in
   Hanford,  California.    Preliminary
   Report, Department of  Civil  and
   Environmental         Engineering,
   University of Houston (1986).
Clifford,   D.A.     Ion-exchange  and
   Inorganic    Adsorption.      Water
   Quality and Treatment, Chapter 9,
   5th edition, McGraw Hill (1999).
Clifford, D.A. and Lin, C.C. Arsenic(III)
   and   Arsenic(V)  Removal   from
   Drinking Water in San Ysidro, New
   Mexico,        EPA/600/S2-91/011,
   USEPA, Cincinnati, Ohio (1991).
Clifford,  D.A. and Zhang, Z.  Arsenic
   chemistry  and speciation.   Proc.
   1993   Water  Quality  Technology
   Conference, AWWA, Denver,  CO
   (1994).
Clifford,  D.A.  et al.   Final Report:
   Phases 1 and 2, City of Albuquerque
   Arsenic   Study,   University   of
   Houston—Department of  Civil and
   Environmental         Engineering,
   Houston, TX (1997).
Clifford,  D.A.  et al.  Arsenic Removal
   by Ion-exchange With and Without
   Brine   Reuse.     Proc.  AWWA
   Inorganic Reductants Workshop, San
   Antonio, TX(1998a).
Clifford, D.A. et al. Final Report: Phase
   3,  City of Albuquerque  Arsenic
   Study,  University  of Houston—
   Department    of    Civil    and
   Environmental         Engineering,
   Houston, TX (1998b).
Driehaus,   W.  et  al.  Oxidation  of
   Arsenate(III)   with    Manganese
   Oxides  in  Water Treatment.  Water
   Research, Vol. 29, No.  1, p 297-305
   (1995).
Everett, K.G. and Skoog, D.A.  Study of
   Uncatalyzed      Oxidation     of
   Arsenic(III)  by   Cerium(IV)   in
   Perchloric Acid Medium. Analytical
   Chemistry,  Vol. 43, No. 12, p 1541-
   1547(1971).
Ferguson, J.F., and Gavis, J. A Review
   of  the  Arsenic  Cycle hi Natural
   Waters.  Water Research, 6:1259-
   1274(1972).
Frank,  P.L.  Arsenic(III) Oxidation and
   Removal from Drinking Water.  MS
   Thesis.   Department  of Civil  and
   Environmental         Engineering,
   University of Houston (1984).
Frank,  P.L., and Clifford, D.A.  Arsenic
   (III) Oxidation and Removal  from
   Drinking Water.  Summary Report,
   EPA/600/S2-86/021 (1986).
Ghurye,    G.L.   et   al.       Iron
   Coagulation/Microfiltration      for
   Arsenic  Removal.  Proc.  AWWA
   Inorganic Reductants Workshop, San
   Antonio, TX (1998).
Ghurye, G.L. et al.  Combined Arsenic
   and  Nitrate  Removal   by  Ion-
   exchange. J American  Water Works
   Association, Vol.  91, No. 10, p 85 -
   96 (1999).
                                      45

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Gupta, B. et al. Kinetics and Mechanism
   of the Silver(I)-catalyzed Oxidations
   of  Arsenic(III)  and  Antimony(III)
   with  Peroxodiphosphate  hi  Acetate
   Buffers.    Journal   of   Chemical
   Society, Dalton Transactions, No. 9,
   pi 873-1876 (1984).
Hathaway,    S.W.   and   Rubel,   F.
   Removing Arsenic  from Drinking
   Water.   J American Water Works
   Association,  Vol.  No.  79,  No.8
   (1987).
Hering, J.G. et al.  Arsenic removal by
   ferric chloride.  J  American Water
   Works Association,  Vol. 88, No. 4, p
   155 -167 (1996a).
Hering, J.G. et al.  Arsenic Removal by
   Enhanced     Coagulation     and
   Membrane  Processes.    (90706).
   AWWARF, Denver (1996b).
Johnson, D. C. and Bruckenstein, D.C.
   A  Study  of the  Kinetics  of  the
   Oxidation   of  Arsenic(III)   by
   Electrogenerated Iodine in Alkaline
   Media.   Journal   of   American
   Chemical Society, Vol. 90, No. 24, p
   6592-6596 (1968).
Khoe,   G.H.  et   al.     Photoassisted
   oxidation  of species in  solution.
   U.S.  Patent No.  5,688,378 (1997).
Khoe,  G.H. et al.   Removal of Arsenic
   Using Advance  Oxidation Processes.
   Presented at the 2000 SME  Annual
   Meeting & Exhibit, Salt Lake City,
   Utah (2000).
Knocke, W. et al.  Alternative oxidants
   for the removal of soluble iron and
   manganese. Prepared for the AWWA
   Research  Foundation, Denver,  CO
   (1990).
Mohan,   D.   et   al.   Kinetics   and
   Mechanism  of the Oxidation  of
   Arsenic(IH) by Hexacyanoferrate(III)
    in   Alkaline  Medium.  Inorganic
   Chemistry, Vol.  16, No. 5, p 1020-
   1029 (1977).
Moore, J. N. et al. Reaction Scheme for
   the Oxidation of As(III) to As(V) by
   Birnessite. Clays and Clay Minerals,
   Vol. 38, No. 5, p 549-555 (1990).
MSB Technology Applications, Inc. and
   Australian  Nuclear   Science   &
   Technology Organization.    Final
   Report—Arsenic          Oxidation
   Demonstration Project for US EPA
   Mine Waste Technology  Program
   (MWTP)  Activity  III,  Project  7
   (1997).
Nishida, S.  and Kimura, M.   Kinetic
   Studies of the Oxidation Reaction of
   Arsenic(III)  to   Arsenic(V)   by
   Peroxodisulphate Ion in  Aqueous
   Alkaline Media Journal of Chemical
   Society, Dalton Transactions, No. 2,
   p 357-360 (1989).
Oscarson, D. W.  et  al.  Kinetics  of
   Oxidation of  Arsenite by  Various
   Manganese  Dioxides. Soil  Science
   Society of America, Vol.  47, p 644-
   648 (1983).
Scott, M.J. and Morgan, J.J. Reactions at
   Oxide  Surfaces.  1.  Oxidation  of
   As(III)   by  Synthetic  Birnessite.
   Environmental     Science     and
   Technology, Vol. 29, No. 8, p 1898-
   1905 (1995).
Sen  Gupta,  K.K.  and Chakladar, J.K.
   Kinetics  of  the  Chromic   Acid
   Oxidation of Arsenic(III). Journal of
   Chemical      Society,      Dalton
   Transactions,  No.  2, p  222-225
   (1989).
Simms,  J.  et  al.   Arsenic  Removal
   Studies and the design of a 20,000
   m3 per day plant  in  the UK. Proc.
   AWWA    Inorganic    Reductants
    Workshop,    Albuquerque,   NM
    (2000).
                                       46

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Standard Methods  for the Examination
   of  Water  and Waste-water,  18th
   Edition, (1992).
U.S. EPA.   Preparation aids for the
   development  of RREL Category III
   Quality  Assurance  Project  Plans.
   Office     of    Research     and
   Development,    Risk    Reduction
   Engineering Laboratory, Cincinnati,
   OH (1991).
White,  G.C.     The   Handbook  of
   Chlorination.   2nd Edition,  Van
   Nostrand Reinhold Co., NY (1986).
                                      47

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  Appendix A.  Preconditioning and Regeneration of IX Filter
A.I  Manufacturer-
Recommended Preconditioning
(1)  Center the filter on the base of a
    vacuum filter holder.
(2) Wet the filter with 10 mL of acetone
    for 30 sec, and then apply vacuum to
    dry the filter.
(3) Add 10 mL of isopropanol and allow
    filter  to soak for 30 sec.   Apply
    vacuum to dry the filter.
(4)  Finally,  add 10 mL of methanol.
    Draw  approximately  3-4  mL  of
    methanol  through the filter  under
    vacuum.  Vent  vacuum  and  allow
    filter allowed to soak in methanol for
    60 seconds.  Reapply vacuum  and
    add 30 mL of reagent grade water to
    rinse   methanol  from  the  filter.
    Ensure that the filter does not dry by
    leaving 3-5  mL of reagent  grade
    water on the filter.

A.2   IX Filter  Regeneration—
       UHMethod
All preconditioned and used filters were
regenerated (converted  to the  chloride
form)  according   to  the  procedure
outlined below.
(5)  Mount the  filter on  the base of a
    vacuum  filtration  apparatus  and
    place a 0.22  um filter (Millipore, 47
    mm,  Type  GS) on top  of the IX
   Empore filter.  Add 20 mL of 1 M
   HC1 and pull approximately 3-4 mL
   of HC1  through  the  filter  under
   vacuum.  Vent the vacuum and allow
   the filter to  soak for   about  60
   seconds.  Reapply  vacuum to draw
   the remaining acid through the filter
   at a flow rate of 20 mL/min or less.
(6)  Add  20 mL of 1  M NaCl and pull
   approximately  3-4  mL  of the salt
   solution through the filter. Vent the
   vacuum and allow the filter to soak
   for about  60  seconds.    Reapply
   vacuum and draw the remaining salt
   solution through the filter at  a flow
   rate of 20 mL/min or less.
(7)  To rinse the concentrated NaCl out
   of the filter, add 20 mL of 0.005 M
   NaCl  and draw about 15 mL through
   the filter at a flow rate of 20 mL/min
   or less.  The IX filter is now ready to
   use for  the As(III)/(V)  speciation
   tests.
(8)       Once   preconditioned   and
   regenerated, the filter should  not be
   allowed  to  dry  for an extended
   period of time.  One way  to prevent
   drying is to place the  filter in a
   beaker  or  a  sealed  plastic  bag
   containing a 0.005 M NaCl solution.
                                      48

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          Appendix B.  Testing Oxidation Reactor Set-up
The procedures followed for testing the
reactor    set-up    for    As(V)/As(III)
separation are described in this section.

(1) Pour 500 mL of the synthetic water
   containing either  As(IH)  or  As(V)
   into the oxidation reactor.  Close the
   reactor.
(2)   Place the  IX filter  in the filter
   holder. Place a 0.22 urn filter on top
   of the IX filter and assemble the on-
   line filter holder.
(3) Pressurize the reactor to 15 psi using
   nitrogen gas.  Open outlet valve and
   allow  the  test  solution  to   flow
   through the filters.
(4)   Discard the  first 10 mL  of the
   effluent.   Collect the  rest of the
   effluent in 10 (or 25)  mL aliquots.
   Acidify the 10 mL aliquots using 50
   uJL of cone, nitric  acid and store at 4
   °C  until ready for analysis.  After
   collecting the desired volume of the
   filter effluent, close the outlet valve.

The test is now completed.  To prepare
for the  next  test, perform the following
procedures.

(5) Vent the nitrogen gas pressure and
   disassemble    the  filter   holder.
   Transfer the  IX filter along with the
   0.22 urn filter  atop it to  a vacuum
   filtration apparatus.
(6)  Regenerate the IX filter as outlined
   in the  Filter  Regeneration Method
   described in Appendix A.
(7)  Simultaneously, clean the reactor by
   rinsing four times with 250 mL of DI
   water. Then fill the reactor with 500
   mL  of DI water.  Close the reactor
   and  pressurize  to  15 psi.   Allow
   approximately 100 mL of flow from
   the reactor through an empty filter
   holder.   This rinses  out the  tubing
   between the reactor and  the filter
   holder, the filter holder itself, and the
   final section of tubing  between the
   filter  holder   and  the   fraction
   collector.
(8)  Discard the used 0.22 jam filter from
   step 7 and transfer the regenerated IX
   filter into the  rinsed in-line filter
   holder.   Place a new 0.22 um filter
   on top of the IX filter. Assemble the
   filter holder together and connect  to
   the   oxidation  reactor.      Allow
   approximately  200  mL of the  DI
   water to pass through the assembled
   filter holder.  This is an  additional
   rinse of all the tubing, the filters, and
   the filter holder. The IX filter is now
   ready  to   speciate  As(III)/As(V).
   Discard  the remaining  DI  water  in
   the reactor. The reactor is now ready
   to receive a fresh test solution.
                                        49

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               Appendix C. Variable-DO Experiments
C.1   High-DO          Chemical
Oxidation Experiments
The experimental procedure for the high-
DO oxidation experiments, which were
run  in duplicate,  is  outlined  below.
Briefly, each experiment was performed
in  duplicate  along  with   a   control
experiment for each  set of  duplicates.
The control experiment was  performed
in  exactly the  same manner  as the
oxidation experiment except that  no
oxidant was present.

(1) Prepare 2 L of the test solution with
    the   appropriate  concentration  of
    As(III). Rinse the reactor twice with
    50 mL of the test solution.  Then
    place 500  mL of the test  solution in
    the reactor.   Close  the reactor and
    pressurize to 15  psi with nitrogen
    gas. Start the magnetic stirrer.
(2)  Place a  conditioned,  chloride-form
    IX filter, fresh or regenerated,  into
    the in-line filter holder.  Place a 0.22
    jam filter on top of the IX filter, and
    assemble  the  in-line  filter  holder.
    Set the tuner on the fraction collector
    at 0.1 min, i.e., 6 seconds.
(3)  Inject the required quantity of the
    oxidant. Immediately, open the outlet
    valve allowing the  test solution to
    flow through the IX filter into one of
    the   15-mL  sample  tubes  in the
    fraction collector.   (The flow rate
    obtained  using  15 psi  nitrogen gas
    pressure   was   approximately   20
    mL/min).
(4) Discard the first 4 mL (12 seconds)
    of the effluent.  Start the  fraction
    collector  and collect samples (~ 2
    mL aliquots) every six seconds for
   the  first  minute  of the  reaction.
   (Samples  were  collected  in  the
   intervals of 12-18, 18-24, 24-30 sec,
   etc.  were  labeled with the median
   tune of sampling as 15, 21,  27 sec
   and  so on).  Then stop the flow by
   closing the outlet valve  and stop the
   fraction collector.  Restart the flow
   by opening the outlet valve at 1 min
   57 sec and allow flow to waste up to
   2 min 07  sec.  Collect  flow  for the
   next 10 sec, i.e., from 2 min 07 sec
   to 2  min 17 sec (median sample time
   of 2 min  12 sec (132 sec) from the
   start of the reaction). Stop the flow
   and  repeat to  get samples  at 192,
   252, and  312  sec.   Acidify the 2.0
   mL  aliquots using 10  uL of cone.
   nitric acid and store at 4 °C until
   ready for analysis.
(5)   At the end of 317 sec, close the
   outlet  valve.   The  experiment  is
   complete at this point.

To prepare for the duplicate, follow the
   procedure described below.

(6) Vent the  nitrogen gas pressure and
   disassemble   the   filter    holder.
   Transfer the IX filter along with the
   0.22 |j.m  filter atop  it to a vacuum
   filtration apparatus.
(7) Regenerate the IX filter as outlined in
   the  IX   Regeneration  Method  in
   Appendix A.
(8)   Clean the reactor  by  rinsing four
   times with  250  mL of  DI  water.
   Then fill the reactor with 500 mL of
   DI  water.  Close the  reactor  and
   pressurize.    Allow approximately
                                        50

-------
    100 mL  of flow from the reactor
    through an empty filter holder.
(9)  Transfer the regenerated IX filter into
    the in-line filter holder. Place a new
    0.22 mm filter on top of the IX filter.
    Assemble  the filter holder together
    and connect to the oxidation reactor.
    Allow approximately 200 mL of the
    DI  water  to  pass  through  the
    assembled filter holder.  Collect the
    last 10 mL of rinse  and  analyze as
    reactor blank. Discard the remaining
    DI water in the  reactor.  The reactor
    is now ready to receive a fresh test
    solution.
(10)  Duplicate the  same experiment by
    following steps 1 through 5.

After completing the repeat experiment,
a control  run was  performed to  verify
that no oxidation occurred in the absence
of oxidant and that no residual oxidant
was left in the reactor, i.e., to verify that
the   rinsing   procedures  used   were
effective in purging the entire reactor
set-up of any leftover oxidant.
(10)  Once again,  clean the reactor as
    described before and fill  with 500
    mL of  test  solution  spiked with
    As(III).    Close  the  reactor  and
    pressurize   with   nitrogen    gas.
    Regenerate and  place the IX filter in
    the filter holder. Start the magnetic
    stirrer.  Start the timer and allow 5
    minutes to pass before opening the
    outlet   valve  and   allowing  flow
    through the filter holder.  Waste the
    first 4 mL and collect the next  10 mL
    of flow and analyze for arsenic. This
   sample served as the control for the
   set of repeat experiments.

C.2   Low-DO           Chemical
Oxidation Experiments
The low-DO experiments were necessary
only when dissolved iron or sulfide was
added to the synthetic test water.  The
low-DO experiments were performed  in
the  same manner  as  the  high-DO
experiments except that the rinse water
and the synthetic water was sparged with
extra-dry-grade nitrogen  gas to produce
a low-DO synthetic water containing <
0.1 mg/L of dissolved oxygen).

The  additional steps (to the procedure
for the high-DO oxidation experiments)
necessary  to  perform  the   low-DO
experiments are described below.

(1) Add 500  mL of the test solution  to
   the reactor and  close  the reactor.
   Sparge with extra dry grade nitrogen
   gas for 30 minutes.
(2) Briefly stop sparging and add the
   appropriate dose of  dissolved  iron
   (Fe(II)) or sulfide.   In the case  of
   dissolved iron, resume  sparging for
   about  5 minutes  and then close the
   reactor while maintaining  nitrogen
   pressure.     However,   after  the
   addition of sulfide, resume sparging
   very  briefly (no more than 5-15
   seconds to prevent sulfide  stripping)
   and  immediately close the reactor.
   Pressurize with nitrogen  gas to  15
   psi.       Perform   the    oxidation
   experiment as described before.
                                        51

-------
           Appendix D.  Solid-Phase (Filox) Experiments
The  procedures  used for the various
Filox experiments are described in detail
hi this appendix.  The procedures used
for the variable-EBCT and variable-pH
experiments was the same.  In order to
perform  Filox experiments  with  low-
DO,  the oxidation reactor  was used.
Essentially, as La the chemical oxidation
experiments, the low-DO synthetic water
was prepared in the oxidation reactor as
described in Appendix C and instead of
adding  any  chemical oxidant to the
reactor, the reactor was maintained under
nitrogen  pressure  and  the  low-DO
synthetic water was  pumped from the
reactor to the Filox column.

D.I   Variable-EBCT         and
Variable-pH Experiments
(1) Two  liters (2L) of  synthetic water
   (previously sparged with air for 15
   min)  containing 50 \igfL  of As(III)
   was prepared in a  2-L  volumetric
   flask.    The  average  DO of this
   synthetic water was 8.2 mg/L.
(2) The high-DO synthetic  water  was
   then  pumped from  the  volumetric
   flask through the Filox column.  The
   flow rate was adjusted to the desired
   setting  (starting  with  16  mL/min,
   EBCT of 0.75 mm) following which
   5 BV (60 mL) of the synthetic water
   was  passed   through  the column.
   Then, approximately 25  mL of the
   Filox column effluent was collected,
   acidified,  and analyzed  as  Total
   Effluent  As.     Flow  was   then
   switched to the DC filter holder and
   three  consecutive  1-BV  intervals
   were   speciated,    acidified,   and
   analyzed for unoxidized As(III).
To prepare the filter for the next EBCT
or pH,  the  IX filter was  regenerated
according to the procedure described in
Appendix A.  To test the next variable,
the  flow rate was  reset to the desired
value and steps (1) - (2) were repeated.

The  procedure  for the  variable-pH
experiments  was the same as for the
variable-EBCT experiments except that
the pH of the feed solution was adjusted
to the desired  level (6.3, 7.3, and 8.3)
using 1M HC1 solution.

After the  desired experiments   were
completed,  flow  through the   Filox
column was stopped.  The remaining
contents of  the volumetric flask  were
then  speciated  for  As(III)/As(V)  to
ensure that all of the arsenic that was fed
to the Filox column was As(III).

D.2  Low-DO Filox Experiments
(1)   500 mL of synthetic  water, without
   any  sulfate,  was sparged for  30
   minutes    with    extra-dry-grade
   nitrogen gas to strip out the dissolved
   oxygen.  It was then pumped through
   the Filox-media column and through
   the IX filter connected to the  outlet
   of the column.  After  purging the
   media and  the  filter holder  with
   approximately  350 mL of the low-
   DO water, the reactor was emptied
   and   refilled   with   the    usual
   composition synthetic water (Table
    1).  Sulfate was excluded because the
   Empore filter has a finite speciation
   capacity  of approximately 100-150
                                       52

-------
mL before it is exhausted, primarily
by sulfate, and requires regeneration.
(2)    After  30  minutes,  nitrogen
sparging was briefly stopped and the
appropriate  amount of As(III) and
reductant were added  to the sparged
water.   Sparging was resumed and
continued for 15-20  more  minutes
and  the low-DO  water  was  then
pumped through the  Filox media
column  with  continued  sparging.
When  testing the  nitrogen-sparged
synthetic water  in  the  presence  of
sulfide, no sparging was performed
after sulfide  had been added to the
test water.  Rather the reactor was
sealed   and  maintained  under   a
nitrogen atmosphere.   Once sulfide
was  introduced  into  the synthetic
water, sparging was stopped to avoid
stripping sulfide  from the synthetic
water.
(3)  After 5 BV (60 mL) had passed
through the Filox media, an effluent
sample  collected at the outlet of the
Filox  column,  was  acidified and
analyzed  for "Total  Effluent As"
exiting the Filox column.  The flow
was  then switched  to the IX filter.
The  first 5 mL of flow from the IX
filter  was  wasted  and  then three
consecutive  1-BV  samples  were
collected, acidified with  cone nitric
acid, and analyzed for arsenic. A 25-
mL sample from the closed  reactor
was    collected,    acidified,    and
analyzed for feed As.   The  reactor
was then emptied and rinsed with DI
water  and  prepared for the  next
experiment.
(4) To verify that all of the  arsenic
fed to the Filox column was As(III),
the oxidation reactor was filled with
synthetic water and sparged  for 30
minutes followed by the  addition of
an appropriate amount of As(III) and
reductant,   if  any.   Sparging  was
continued for a further 15-20 minutes
(except in  the presence  of sulfide)
and the synthetic water containing 50
ug/L As(III) was pumped from the
reactor straight to the IX filter.  The
effluent from  the  IX  filter  was
collected, acidified, and analyzed as
Control As(III).  Such controls were
performed    (one    control/set   of
duplicates)  for the varying pH  and
EBCT experiments as well as each of
the      interfering      reductant
experiments.
                                    53

-------
               Appendix E.  Chlorine Oxidation Data
Run#
E15.01-
E15.03-
E15.05-
E15.07-
E15.09-
E15.ll-
E15.13-
E15.15-
E15.17-
15.02:
15.04:
15.06:
15.08:
15.10:
15.12:
15.14:
15.16:
15.18:
E15.19-15.20:

E15.21-15.22:
      Description
No interfering reductants:
No interfering reductants:
No interfering reductants:
Mn(II) = 0.2 mg/L; Dose =
Fe(II) = 0.3 mg/L; Dose =
Fe(II) = 2.0 mg/L; Dose =
Sulfide =1.0 mg/L; Dose
Sulfide = 2.0 mg/L; Dose
TOC = 6.9 mg/L; Dose =
Water
No interfering Reductants
pH = 8.3
No interfering reductants:
Dose = 3 x As(III); pH = 8.3
Dose = 3 x As(III); pH = 7.3
 Dose = 3xAs(IH);pH = 6.3
= 10xAs(III);pH = 8.3
 10 x As(III); pH = 8.3
 10xAs(III);pH = 8.3
= 10xAs(III);pH = 8.3
= 10xAs(III);pH = 8.3
10 x As(III); pH = 8.3; Lake Houston

 As(IH) = 1000 ug/L; Dose = 3 x As(III);

5 °C; Dose = 3 x As(III); pH = 8.3
                                      54

-------
Table E-l. Residual As(III) Cone, vs Time for Chlorine Experiments.
Sample
Interval
(sec)

12-18
18-24
24-30
30-36
36-42
42-48
48-54
54-60
60-66
66-72
127-137
187-197
247-257
307-317

Median
Time
(sec)
0
15
21
27
33
39
45
51
57
63
69
132
192
252
312

15.01
50.0
1.6
1.2
0.6
0.5
0.3
0.5
0.2
0.5
0.3
0.5
0.1
0.2
0.1
0.2

15.02
50.0
1.6
1.2
0.6
0.5
0.3
0.5
0.2
0.5
0.3
0.5
0.1
0.2
0.1
0.2

B
15.03
50.0
2.2
1.3
0.7
0.1
0.5
0.6
0.5
0.5
0.2
0.2
0.1
0.1
0.1
0.2

.esidual /
15.04
50.0
2.1
1.3
0.6
0.3
0.2
0.3
0.1
0.6
0.2
0.1
0.1
0.2
0.5
0.2

is(III) Co
15.05
50.0
14.0
9.1
6.1
3.7
2.3
1.4
0.7
0.1
0.3
0.1
0.0
0.2
0.1
0.3

tic., ug/L
15.06
50.0
14.0
9.6
8.1
2.9
1.6
1.3
0.7
0.3
0.5
0.1
0.6
0.2
0.1
0.1

for Run t
15.07
50.0
4.0
2.5
2.0
1.6
1.3
1.1
0.8
0.6
0.3
0.3
0.3
0.1
0.3
0.3

f
15.08
50.0
4.6
3.1
1.4
1.6
1.1
0.1
0.5
0.1
0.1
0.3
0.3
0.3
0.6
0.3

15.09
50.0
0.5
0.
0.
0.
0.
0.
0.1
0.1
0.1
0.1
0.1
0.2
0.8
0.3

15.10
50.0
1.3
0.2
0.1
0.3
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1

Table E-l. Continued.
Sample
Interval
(sec)

12-18
18-24
24-30
30-36
36-42
42-48
48-54
54-60
60-66
66-72
127-137
187-197
247-257
307-317
Median
Time
(sec)
0
15
21
27
33
39
45
51
57
63
69
132
192
252
312
15.11
50.0
0.8
0.2
0.
0.
0.
0.
0.
0.
0.
0.1
0.1
0.1
0.1
0.1
15.12
50.0
1.2
0.1
0.1
0.
0.
0.
0.
0.
0.
0.1
0.1
0.1
0.1
0.1
R
15.13
50.0
18.0
14.0
7.2
6.1
5.5
3.5
2.7
1.0
0.8
0.4
0.8
0.8
1.0
0.6
.esidual /
15.14
50.0
20.0
15.0
8.9
6.4
4.0
3.2
2.4
1.1
0.8
0.6
0.3
0.3
0.8
0.8
LS(HI) Co
15.15
50.0
24.0
19.0
15.0
12.0
8.2
5.3
1.7
1.5
1.1
0.5
0.3
0.5
0.6
0.6
nc., ug/L
15.16
50.0
26.0
19.0
16.0
14.0
11.0
7.2
3.2
2.1
1.7
0.9
0.3
0.1
0.3
0.1
for Run ^
15.17
50.0
1.5
1.5
.2
.2
.0
.2
.3
.2
.2
.2
2.3
1.5
2.3
1.0
[
15.18
50
2.8
2.8
1.9
1.3
1.0
0.1
1.3
2.1
2.8
3.6
4.1
2.8
3.7
3.6
15.19
1000
10.0
3.7
1.1
3.1
4.1
4.3
3.5
3.7
4.5
3.3
3.5
2.1


15.20
1000
7.5
1.7
5.3
3.3
2.5
2.9
4.9
0.9
3.5
1.5
3.1
2.5


                                       55

-------
Table E-l. Continued.
Sample
Interval
(sec)

12-18
18-24
24-30
30-36
36-42
42-48
48-54
54-60
60-66
66-72
127-137
187-197
247-257
307-317
Median
Time
(sec)
0
15
21
27
33
39
45
51
57
63
69
132
192
252
312
Rui
H&
15.21
50
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
i#
IL
15.22
50
0.1
0.1
0.1
0.1
0.1
o.i
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
                                       56

-------
Appendix F. Permanganate Oxidation Data
Run #
E16.01
E16.03
E16.05
E16.07
E16.09
E16.1 1
E16.13
E16.15
E16.17
E16.19
       16.02
       16.04
       16.06
       16.08
       16.10
       16.12
       16.14
       16.16
       16.18
       16.20
E16.21-16.22
      Description
No interfering reductants:
No interfering reductants:
No interfering reductants:
Mn(II) = 0.2 mg/L; Dose
Fe(II) = 0.3 mg/L; Dose =
Fe(II) = 2.0 mg/L; Dose =
Sulfide - 1.0 mg/L; Dose
Sulfide = 2.0 mg/L; Dose
TOC = 6.9 mg/L; Dose =
No interfering Reductants
As(IH);pH = 8.3
No interfering reductants:
                       Dose = 3 x As(III); pH = 8.3
                       Dose = 3 x As(III); pH = 7.3
                       Dose = 3 x As(III); pH = 6.3
                         10 x As(III); pH = 8.3
                        10 x As(III); pH = 8.3
                        10 x As(III); pH = 8.3
                       = 10 x As(III); pH = 8.3
                       = 10 x As(III); pH = 8.3
                       10 x As(III); pH = 8.3
                       ; As(III) = 1000 ug/L; Dose = 3 x

                       5 °C; Dose = 3 x As(III); pH = 8.3
                          57

-------
Table F-l. Residual As(III) Cone, vs Time for Permanganate Experiments.
Sample
Interval
(sec)

12-18
18-24
24-30
30-36
36-42
42-48
48-54
54-60
60-66
66-72
127-137
187-197
247-257
307-317
Median
Time
(sec)
0
15
21
27
33
39
45
51
57
63
69
132
192
252
312
16.01
50.0
1.9
1.2
0.7
0.3
0.2
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
16.02
50.0
2.6
1.8
1.2
0.5
0.2
0.1
0.2
0.3
0.1
0.1
0.1
0.1
0.1
0.1
B
16.03
50.0
2.1
1.4
0.7
0.2
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
.esidual A
16.04
50.0
1.7
0.8
0.6
0.3
0.2
0.2
0.1
0.2
0.1
0.1
0.1
0.1
0.1
0.1
is(III) Co
16.05
50.0
6.0
3.9
2.0
0.9
0.7
0.2
0.7
0.
0.
0.
0.
0.
0.1
0.1
nc., ug/L
16.06
50.0
6.7
5.3
3.9
1.9
0.6
0.2
0.2
0.2
0.1
0.2
0.2
0.3
0.5
0.2
for Run it
16.07
50.0
2.4
1.5
0.7
0.3
0.
0.
0.
0.
0.
0.1
0.1
0.3
0.3
0.3
[
16.08
50.0
3.0
1.3
0.4
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.1
0.1
16.09
50.0
3.1
1.5
0.7
0.1
0.
0.
0.
0.
0.
0.
0.
0.
0.
0.
16.10
50.0
3.2
1.8
0.6
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
Table F-l. Continued.
Sample
Interval
(sec)

12-18
18-24
24-30
30-36
36-42
42-48
48-54
54-60
60-66
66-72
127-137
187-197
247-257
307-317
Median
Time
(sec)
0
15
21
27
33
39
45
51
57
63
69
132
192
252
312
16.11
50.0
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.0
0.1
0.1
0.1
0.1
0.1
0.1
16.12
50.0
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
R
16.13
50.0
5.7
5.0
4.3
4.0
3.2
2.9
2.6
1.4
1.0
0.6
0.3
0.3
0.4
0.4
.esidual A
16.14
50.0
6.9
5.5
4.7
4.1
3.8
3.1
2.1
1.4
1.3
0.6
0.6
0.6
0.1
0.6
is(III) Co
16.15
50.0
11.8
10.7
9.0
6.5
5.7
4.4
3.7
2.4
1.8
1.4
0.4
0.1
0.5
0.6
nc., ug/L
16.16
50.0
10.7
9.0
8.2
7.2
5.1
3.2
.5
.4
.4
.3
.1
0.6
0.6
0.6
for Run ri
16.17
50.0
1.5
0.0
1.4
1.5
1.0
0.4
3.4
0.2
1.5
1.9
1.6
1.4
2.1
0.8
f
16.18
50.0
0.4
0.4
0.2
0.1
0.1 ,
0.1
0.1
0.1
0.2
0.1
1.2
1.5
2.1
2.8
16.19
1000
8.9
1.5
1.9
2.9
3.3
2.5
1.7
2.9
2.9
2.3
0.9
0.9


16.20
1000
13.0
0.9
2.5
3.7
1.7
1.3
2.9
0.9
0.9
3.5
1.7
2.9


                                       58

-------
Table F-l. Continued.
Sample
Interval
(sec)

12-18
18-24
24-30
30-36
36-42
42-48
48-54
54-60
60-66
66-72
127-137
187-197
247-257
307-317
Median
Time
(sec)
0
15
21
27
33
39
45
51
57
63
69
132
192
252
312
Rui
Hg
16.21
50.0
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
i#
IL
16.22
50.0
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
                                       59

-------
          Appendix G.  Chlorine Dioxide Oxidation Data

Run #              Description
E17.01-17.02: No interfering reductants: pH 8.3
E17.03-17.04: No interfering reductants: pH 7.3
E17.05-17.06: No interfering reductants: pH 6.3
E17.07-17.08: Mn(II) = 0.2 mg/L; Dose = 3 x As(III); pH = 8.3
E17.09-17.10: Fe(II) = 0.3 mg/L; Dose = 3 x As(III); pH = 8.3
E17.11-17.12: Fe(II) = 2.0 mg/L; Dose = 3 x As(IH); pH = 8.3
E17.13-17.18: Oxidation of Fe(II); Repeat of Knocke (1990) experiments
             Not included in Table G-l
E17.19-17.20: Repeat of E17.01-17.02; No interfering reductants: pH 8.3
E17.21-17.22: No interfering reductants: pH 8.3; 3 x C12 @ t=l'45"
E17.23-17.24: No interfering reductants: pH 8.3; Deaerated Test Solution
E17.25-17.26: No interfering reductants: pH 8.3; Dose = 10 x As(III)
E17.27-17.28: No interfering reductants: pH 8.3; Dose = 100 x As(III)
                                       60

-------
Table G-l. Residual As(III) Cone, vs Time for Chlorine Dioxide Experiments.
Sample
Interval
(sec)

12-18
18-24
24-30
30-36
36-42
42-48
48-54
54-60
60-66
66-72
127-137
187-197
247-257
307-317
Median
Time
(sec)
0
15
21
27
33
39
45
51
57
63
69
132
192
252
312
17.01
50.0
38.9
37.7
37.9
37.5
38.9
37.7
37.5
37.5
38.4
38.6
39.6
37.2
38.4
38.0
17.02
50.0
42.0
40.6
40.3
40.3
40.8
39.4
39.9
39.9
40.5
40.6
40.3
39.4
39.1
39.1
F
17.03
50.0
47.0
46.8
46.8
47.5
46.6
47.3
46.3
46.8
46.5
47.2
47.8
47.3
47.5
47.3
.esidual A
17.04
50.0
48.4
49.0
46.8
47.0
47.3
47.0
47.2
47.2
47.5
47.0
46.3
46.3
45.8
45.8
LS(III) Co
17.05
50.0
47.0
47.3
46.5
46.1
45.6
47.7
48.4
46.8
46.5
46.7
47.5
47.7
46.3
48.2
nc., jig/L
17.06
50.0
49.0
49.0
48.7
48.4
48.0
48.0
47.0
47.0
46.8
46.3
46.3
44.6
44.6
45.4
for Run i
17.07
50.0
37.2
35.6
35.3
31.7
35.1
34.4
34.3
34.1
34.3
34.6
34.3
33.7
33.6
34.6
^
17.08
50.0
36.3
35.8
35.3
33.9
33.6
33.9
33.2
31.2
32.7
32.9
33.2
33.4
32.2
33.2
17.09
50.0
30.8
29.3
28.4
27.0
26.7
27.2
27.5
26.7
26.5
27.0
27.0
27.0
28.0
26.7
17.10
50.0
29.4
26.8
26.3
26.7
27.0
26.7
26.0
25.8
24.9
25.5
28.0
26.7
27.0
27.0
Table G-l. Continued.
Sample
Interval
(sec)

12-18
18-24
24-30
30-36
36-42
42-48
48-54
54-60
60-66
66-72
127-137
187-197
247-257
307-317
Median
Time
(sec)
0
15
21
27
33
39
45
51
57
63
69
132
192
252
312
17.11
50.0
23.9
21.7
21.5
20.8
20.1
20.3
19.8
20.8
20.8
21.0
21.0
20.6
21.8
19.8
17.12
50.0
21.8
19.8
19.4
17.7
18.0
18.4
18.6
19.1
18.6
19.4
19.4
19.1
18.9
19.1
P
17.19
50.0
35.4
34.8
34.8
35.4
35.4
35.2
34.6
34.8
34.6
35.4
35.4
33.8
34.6
33.3
.esidual A
17.20
50.0
38.8
36.6
38.8
36.4
39.2
39.2
39.0
38.6
38.8
38.6
35.2
36.6
36.0
35.4
LS(III) Co
17.21
50.0
40.4
39.6
38.8
38.4
38.2
37.2
36.6
36.2
36.4
35.8
1.5
0.1
0.1
0.1
nc., ng/L
17.22
50.0
40.2
40.4
39.0
39.6
39.4
38.0
38.2
38.4
37.0
37.4
0.9
0.1
0.1
0.1
for Run tf
17.23
50.0
40.2
39.6
39.2
38.4
38.4
37.6
37.8
37.1
37.3
37.3
37.1
38.0
38.0
37.4
[
17.24
50.0
41.1
40.5
40.2
40.0
39.6
38.4
38.6
38.6
38.2
38.4
38.4
38.4
38.6
39.0
17.25
50
35.5
36.0
35.2
35.2
33.6
33.4
32.0
31.0
31.5
29.9
30.6
29.6
29.9
30.1
17.26
50
36.0
35.9
36.2
34.8
31.8
32.0
32.9
34.3
31.1
30.6
31.8
30.4
29.0
28.5
                                       61

-------
Table G-l. Continued.
Sample
Interval
(sec)

12-18
18-24
24-30
30-36
36-42
42-48
48-54
54-60
60-66
66-72
127-137
187-197
247-257
307-317
Median
Time
(sec)
0
15
21
27
33
39
45
51
57
63
69
132
192
252
312
Rui
V-&
17.27
50.0
26.1
24.2
24.0
23.6
22.8
22.2
22.1
22.4
21.2
20.8
15.6
13.2
12.5
12.1
i#
fL
17.28
50.0
25.2
25.9
24.5
24.3
23.5
21.9
22.8
20.7
18.9
18.4
14.7
14.0
10.9
12.3
                                       62

-------
          Appendix H.  Monochloramine Oxidation Data

Run #              Description
E18.01-18.02: No interfering reductants: pH 8.3: in situ monochloramine
El 8.03-18.04: No interfering reductants: pH 7.3: in situ monochloramine
E18.05-18.06: No interfering reductants: pH 6.3: in situ monochloramine
El8.07-18.08: No interfering reductants: pH 8.3: preformed monochloramine
                                    63

-------
Table H-l. Residual As(III) Cone, vs Time for Monochloramine Experiments
Sample
Interval
(sec)

12-18
18-24
24-30
30-36
36-42
42-48
48-54
54-60
60-66
66-72
127-137
187-197
247-257
307-317
Median
Time
(sec)
0
15
21
27
33
39
45
51
57
63
69
132
192
252
312
18.01
50.0
30.6
30.1
29.4
29.4
29.6
29.9
28.9
29.6
29.8
29.8
29.8
29.1
29.4
29.8
F
18.02
50.0
33.3
33.1
33.6
33.6
31.5
32.2
32.4
32.2
32.4
32.9
31.5
31.5
31.5
31.9
esidual A
18.03
50.0
31.3
31.5
31.3
31.0
30.6
30.5
30.6
31.0
31.3
30.3
29.4
30.1
29.6
30.8
LS(III) Co
18.04
50.0
29.4
29.1
29.1
28.9
28.5
28.2
28.9
28.9
30.5
28.7
28.5
28.2
28.4
28.4
nc., ng/L
18.05
50.0
33.3
30.6
29.1
28.7
28.7
29.1
29.2
30.1
29.8
29.8
30.1
29.1
29.1
29.4
for Run ri
18.06
50.0
31.9
32.6
31.5
30.8
30.5
30.8
30.8
30.5
30.5
31.0
30.1
29.8
29.1
29.6
18.07
50.0
47.6
48.6
48.9
48.9
49.2
49.9
50.2
49.4
49.4
48.7
49.2
50.2
49.1
49.9
18.08
50.0
47.4
49.1
49.6
50.1
49.9
49.1
49.6
50.1
50.6
49.4
49.4
49.1
49.7
50.2
                                     64

-------
                 Appendix I.  Ozone Oxidation Data

Run #             Description
E19.01-19.02: No interfering reductants: Dose = 3 x As(III); pH = 8.3
E19.03-19.04: No interfering reductants: Dose = 3 x As(III); pH = 7.3
E19.05-19.06: No interfering reductants: Dose = 3 x As(III); pH = 6.3
E19.07-19.08: Mn(II) = 0.2 mg/L; Dose = 10 x As(III); pH = 8.3
E19.09-19.10: Fe(II) = 0.3 mg/L; Dose = 10 x As(III); pH = 8.3
E19.11-19.12: Fe(II) = 2.0 mg/L; Dose = 10 x As(III); pH = 8.3
E19.13-19.14: Sulfide = 1.0 mg/L; Dose = 10 x As(III); pH = 8.3
E19.15-19.16: Sulfide = 2.0 mg/L; Dose = 10 x As(III); pH = 8.3
E19.17-19.18: TOC = 6.9 mg/L; Dose = 10 x As(IH); pH = 8.3
E19.19      : Repeat of E19.01-19.02; No interfering reductants: Dose = 3 x As(III);
             pH = 8.3
E19.20      : Repeat of E19.17-19.19; TOC = 6.9 mg/L; Dose = 10 x As(IH); pH = 8.3
E19.21-19.22: TOC = 2.1 mg/L; Dose = 10 x As(III); pH = 8.3
E19.23-19.24: No interfering reductants: 5 °C; Dose = 3 x As(III); pH = 8.3
E19.25-19.26: Repeat of E19.21-19.22; TOC = 2.1 mg/L; Dose = 10 x As(III); pH = 8.3
                                      65

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Table 1-1. Residual As(III) Cone, vs Time for Ozone Experiments.
Sample
Interval
(sec)

12-18
18-24
24-30
30-36
36-42
42-48
48-54
54-60
60-66
66-72
127-137
187-197
247-257
307-317
Median
Time
(sec)
0
15
21
27
33
39
45
51
57
63
69
132
192
252
312
19.01
50.0
1.9
1.2
1.2
1.1
1.1
0.9
0.5
0.7
0.9
0.9
0.9
0.9
0.7
0.7
19.02
50.0
1.1
0.5
0.4
0.
0.
0.
0.
0.
0.1
0.1
0.1
0.1
0.1
0.1
B
19.03
50.0
0.7
0.7
0.5
0.2
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.4
0.1
0.1
.esidual A
19.04
50.0
0.4
0.2
0.1
0.1
0.
0.
0.
0.
0.
0.
0.4
0.1
0.1
0.1
Ls(III) Co
19.05
50.0
1.6
1.1
0.4
0.2
0.1
0.1
0.1
0.4
0.
0.
0.
0.2
0.
0.
nc.,,ug/L
19.06
50.0
0.9
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
for Run ^
19.07
50.0
0.8
0.5
0.5
0.2
0.3
0.5
0.2
0.2
0.1
0.1
0.3
0.1
0.2
0.1
f
19.08
50.0
1.2
0.8
0.5
0.3
0.3
0.2
0.1
0.1
0.1
0.1
0.1
0.2
0.1
0.1
19.09
50.0
0.5
0.2
0.1
0.1
0.2
0.1
0.1
0.1
0.2
0.1
0.1
0.1
0.1
0.1
19.10
50.0
0.2
0.2
0.2
0.1
0.1
0.2
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
Table 1-1. Continued.
Sample
Interval
(sec)

12-18
18-24
24-30
30-36
36-42
42-48
48-54
54-60
60-66
66-72
127-137
187-197
247-257
307-317
Median
Time
(sec)
0
15
21
27
33
39
45
51
57
63
69
132
192
252
312
19.11
50.0
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
19.12
50.0
0.
0.
0.
0.
0.
0.
0.
0.1
0.1
0.1
0.1
0.1
0.1
0.1
R
19.13
50.0
16.4
11.1
8.3
6.1
4.7
3.3
2.5
2.0
2.0
1.3
0.9
0.4
0.2
0.1
esidual A
19.14
50.0
14.0
9.9
6.6
5.2
4.2
2.6
1.1
0.6
0.1
0.1
0.3
0.1
0.2
0.4
LS(III) Co
19.15
50.0
24.4
21.1
18.3
14.9
12.5
10.2
7.3
6.1
5.4
3.3
2.0
2.1
1.3
1.4
nc., ng/L
19.16
50.0
26.4
23.8
19.7
16.1
13.3
12.3
9.2
6.4
5.9
5.1
2.2
1.4
0.9
0.6
for Run *
19.17
50.0
37.8
36.9
34.7
35.4
34.7
35.8
35.6
34.9
35.1
34.9
34.9
35.1
34.9
35.2
19.18
50.0
34.3
34.3
33.8
32.8
32.8
31.9
32.3
31.9
32.1
31.7
31.4
31.2
30.4
31.2
19.19
50.0
3.1
2.6
2.8
2.4
1.5
2.4
1.5
2.1
1.4
1.2
0.8
0.7
0.1
0.3
19.20
50.0
35.7
35.2
35.3
35.9
35.0
34.8
35.5
35.2
34.3
35.7
35.3
35.0
34.6
35.0
                                       66

-------
Table 1-1. Continued.
Sample
Interval
(sec)

12-18
18-24
24-30
30-36
36-42
42-48
48-54
54-60
60-66
66-72
127-137
187-197
247-257
307-317
Median
Time
(sec)
0
15
21
27
33
39
45
51
57
63
69
132
192
252
312
19.21
50.0
3.1
1.8
1.5
1.0
0.8
0.8
0.8
0.8
0.8
1.0
0.1
0.1
0.1
0.1
Residual i
19.22
50.0
1.4
1.0
0.7
0.5
0.5
0.5
0.3
0.1
0.1
0.1
0.1
0.1
0.7
0.1
\s(III) Co
19.23
50.0
3.8
3.1
2.6
2.6
2.4
2.4
2.2
2.4
2.4
2.1
0.1
0.1
0.1
0.1
QC., Ug/L
19.24
50.0
4.3
3.1
3.1
2.8
2.4
2.2
2.1
1.7
1.5
1.0
0.3
0.5
0.8
1.0
for Run #
19.25
50.0
5.0
4.3
3.1
2.7
2.2
0.9
1.2
0.7
1.1
2.2
1.2
1.8
1.4
0.7
19.26
50.0
2.2
0.7
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.1
0.9
0.7
0.9
0.2
                                       67

-------
                 Appendix J.  Filox Oxidation Data

Run #             Description
E20.01 :No interfering reductants: pH = 8.3, EBCT = 0.75 min, High DO
E20.02:No interfering reductants: pH = 8.3, EBCT = 1.5 min, High DO
E20.03:No interfering reductants: pH = 8.3, EBCT = 3.0 min, High DO
E20.04:No interfering reductants: pH = 8.3, EBCT = 6.0 min, High DO
E20.05:RepeatofE20.1
E20.06:Repeat of E20.2
E20.07:RepeatofE20.3
E20.08:Repeat of E20.4
£20.09:2000 BV Filox Run, No interfering reductants: pH = 8.3, EBCT = 0.75
      min, High DO
E20.10:No interfering reductants: pH = 7.3, EBCT = 1.5 min, High DO
E20.1 l:No interfering reductants: pH = 6.3, EBCT = 1.5 min, High DO
E20.12:No interfering reductants: pH = 7.3, EBCT = 1.5 min, Low DO
E20.13:Repeat of E20.12
E20.14:Mn(II) = 0.2 mg/L; pH = 8.3, EBCT = 1.5 min, Low DO
E20.15:Fe(II) = 0.3 mg/L; pH = 8.3, EBCT = 1.5 min, Low DO
E20.16:Fe(II) = 2.0 mg/L; pH = 8.3, EBCT = 1.5 min, Low DO
E20.17:Sulfide = 1.0 mg/L; pH = 8.3, EBCT = 1.5 min, Low DO
E20.18:Sulfide = 2.0 mg/L; pH = 8.3, EBCT = 1.5 min, Low DO
E20.19:TOC = 1.4 mg/L; pH = 8.3, EBCT = 1.5 min, Low DO
E20.20:No interfering Reductants; 5 °C, pH = 8.3, EBCT = 1.5 min, Low DO
E20.21:No interfering Reductants; As(III) = 1000 ug/L, pH = 8.3, EBCT = 1.5
      min, Low DO
E20.22:No interfering Reductants; As(III) = 1000 ug/L, pH = 8.3, EBCT = 1.5
      min, High DO
E20.23:Mn(II) = 0.2 mg/L; pH = 8.3, EBCT = 1.5 min, High DO
E20.24 :Sulfide = 1.0 mg/L; pH = 8.3, EBCT = 1.5 min, High DO
E20.25:Sulfide = 2.0 mg/L; pH = 8.3, EBCT = 1.5 min, High DO
E20.26:TOC = 1.4 mg/L; pH = 8.3, EBCT = 1.5 min, High DO
E20.27:Mn(TI) = 0.2 mg/L; pH = 8.3, EBCT = 6.0 min, Low DO
E20.28:Sulfide = 1.0 mg/L; pH = 8.3, EBCT = 6.0 min, Low DO
E20.29:Sulfide = 2.0 mg/L; pH = 8.3, EBCT = 6.0 min, Low DO
E20.30:TOC = 1.4 mg/L; pH = 8.3, EBCT = 6.0 min, Low DO
                                     68

-------
Table J-l. Residual As(III) vs Effluent As Concentration for Filox Experiments.
Sample
ID
E20.01
E20.02
E20.03
E20.04
E20.05
E20.06
E20.07
E20.08
E20.10
E20.ll
E20.12
E20.13
E20.14
E20.15
E20.16
E20.17
E20.18
E20.19
E20.20
E20.21
E20.22
E20.23
E20.24
E20.25
E20.26
E20.27
E20.28
E20.29
E20.30
Residual As(III)
Concentration
Hg/L
2.0
0.1
0.1
0.4
2.6
1.2
1.2
0.3
0.1
0.1
0.3
0.5
10.9
9.5
9.3
19.3
22.1
10.4
0.8
33.2
49.7
0.8
0.8
1.9
1.2
0.1
0.2
1.4
0.1
Total Effluent
As
Hg/L
MM
MM
NM
NM
27.3
25.9
22.5
18.2
16.9
12.4
24.5
39.4
43.8
40.5
43.1
47.5
45.3
46.4
31.8
55-1.9
499.3
24.7
12.0
11.4
22.3
24.5
21.3
28.8
26.3
MM: Not Measured
E20.09
BV
100
200
300
400
500
600
700
800
900
1000
1100
1200
1300
1400
1500
1600
1700
1800
1900
2000
Residual As(III)
Concentration
Hg/L
2.1
2.0
2.8
3.5
3.3
2.5
2.3
3.0
2.8
3.0
3.2
3.8
2.8
3.2
3.7
3.0
3.7
3.5
3.0
3.5
Total Effluent
As
ug/L
36.8
40.1
41.3
42.1
43.1
40.2
41.6
43.5
42.6
43.3
45.3
44.3
42.6
42.5
44.3
44.2
44.2
44.5
46.2
45.9

                                       69

-------
                 Appendix K. UV Oxidation Data
Run #            Description
E21.01:UV1: Flow Rate = 48 mL/min, Contact Time =
E21.02:UV1: Flow Rate = 96 mL/min, Contact Time =
E21.03:UV1: Flow Rate = 288 mL/min, Contact Time
E21.04:Repeat of E21.03
E21.05:Repeat of E21.02
E21.06:RepeatofE21.01
E21.07:UV1: Flow Rate = 24 mL/min, Contact Time =
E21.08:UV1: Flow Rate = 12 mL/min, Contact Time =
E21.09:UV1: Flow Rate = 12 mL/min, Contact Time =
E21.10:UV1: Flow Rate = 12 mL/min, Contact Time =
E21.11 :UV1: Flow Rate = 310 mL/min, Contact Time
      Sulfite=1.0mg/L
E22.01:UV2: Flow Rate = 310 mL/min, Contact Time
E22.02:UV2: Flow Rate = 209 mL/min, Contact Time
E22.03:UV2: Flow Rate = 104 mL/min, Contact Time
E22.04:UV2: Flow Rate = 52 mL/min, Contact Time =
E22.05:UV2: Flow Rate = 26 mL/min, Contact Time =
E22.06:UV2: Flow Rate = 26 mL/min, Contact Time =
E22.07:UV2: Flow Rate = 26 mL/min, Contact Time =
 6 min, pH = 8.3
•• 3 min, pH = 8.3
= 1 min, pH = 8.3
= 12 min, pH = 8.3
= 24 min, pH = 8.3
= 6 min, pH = 7.3
= 6 min, pH = 6.3
= 0.9 min, pH = 8.3

= 1.6min,pH = 8.3
= 2.3 min, pH = 8.3
= 4.7 min, pH = 8.3
= 9.3 min, pH = 8.3
= 18.7 min, pH = 8.3
= 18.7min,pH = 7.3
= 18.7min,pH = 6.3
                                    70

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Table K-l. Residual As(III) Cone, for UV Experiments.
UV Unit 1
Sample
ID
E21.01
E21.02
E21.03
E21.04
E21.05
E21.06
E21.07
E21.08
E21.09
E21.10
E21.ll
UV Intensity
mW-sec/cm^
11520
5760
1920
1920
5760
11520
23040
46080
46080
46080
1780
Residual As(IH)
Concentration
Hg/L
35.7
42.2
48.8
13.5
21.2
37.3
43.4
47.7
14.5
17.9
0.1
UV Unit 2
Sample
ID
E22.01
E22.02
E22.03
E22.04
E22.05
E22.06
E22.07
UV Intensity
mW-sec/cm2
3380
5760
11520
23040
46080
46080
46080
Residual As(III)
Concentration
Hg/L
28.5
35.8
44.0
47.4
49.2
29.8
36.7
                                        71

-------
                 Appendix L. As(III) Stock Solution
An As(III)  stock solution containing
88.0 mg/L As(III) was prepared in a
synthetic water of a composition similar
to the synthetic  test water  shown  in
Table  2-1  except  that   no   calcium
chloride was added to the stock solution.
Calcium  chloride was not added as the
high pH of the As(III) stock  solution
(due to added bicarbonate  and  silicate)
would have resulted  in the  precipitation
of calcium carbonate.

Approximately 500 mL of reagent grade
water  was added to a  1-L volumetric
flask.  All of the ions  except  calcium
chloride   were  added  to  the  flask.
Finally, the required amount of sodium
m-arsenite (Sigma Chemical  Co.)  was
added   to   give   a   final   As(III)
concentration of approximately 88 mg/L
and  the  volume  of  the As(III) stock
solution was made up to 1.0 L.  The pH
of this As(III)  stock solution  was  9.1.
The stock solution was then refrigerated
(4 °C).  When required, the  necessary
volume of this As(III) stock solution was
added  to the synthetic test  water to
provide a final  As(III) concentration of
50 or 1000 ng/L.
                                       72

-------
                         Appendix M.  QC Data
The   following   QC  samples   were
analyzed:
(1) QC standards
These  were prepared  by the QA/QC
Officer for this project, Anthony Tripp,
and  served as "in-house" QC  check
standards. Four standards were prepared
at a time and any one of these standards
were analyzed  along with the four WAL
standards    during    analysis.    The
concentrations  of  these  QC  standards
were within the range of 1.0-11.0 ug/L
As.   These standards were analyzed
without dilution.   Results from all the
QC samples are shown in Table M-l.

(2) WS Standards
Four WS standards were  preserved in a
0.2 M  nitric acid solution and measured
once during a batch of samples.   The
concentrations of these  standards are
shown in Table 2-3. Standards WS 037,
WS  038,  and WS 041 were  always
diluted    10-fold   before   analysis.
Standard WS 040 was diluted 10- and
20-fold before analysis. Results from all
the WS  samples are shown in Table M-
2.

(3) Spikes
Standards  WS 037, WS  038, and WS
041  were always diluted 10-fold and
spiked with 2.0 ug/L As. Standard WS
041 was diluted 20-fold and then spiked
with 2.0 ug/L As.  Results of arsenic
recoveries  from  all   the  WS-spiked
samples  are shown  hi  Table  M-3.
                                      73

-------
Table M-l. Results of QC Analysis
Run#
E19.13-19.16
E19.13-19.16
E19.13-19.16
E19.13-19.16
E19.13-19.16
E19.13-19.16
E20.1-20.4
E20.1-20.4
E20. 1-20.4
E20.5-20.8
E20.5-20.8
E20.5-20.8
E20. 14-20.22
E20. 14-20.22
E20.14-20.22
E20. 14-20.22
E20.14-20.22
E20. 14-20.22
E20.14-20.22
Number = 19
Run#
E16. -16.6
E16. -16.6
E16. -16.6
E16. -16.6
E16. -16.6
E17. -17.6
E17. -17.6
E17.1-17.6
E17.1-17.6
E17.1-17.6
E17.1-17.6
E17.1-17.6
E18.7-18.8
El 8.7-1 8.8
E18.7-18.8
E18.7-18.8
E18.7-18.8
E18.7-18.8
E18.7-18.8
E18.7-18.8
Number =20
Date
7/9/1999
7/9/1999
7/9/1999
7/9/1999
7/9/1999
7/9/1999
9/25/1999
9/25/1999
9/25/1999
9/28/1999
9/28/1999
9/28/1999
10/26/1999
10/26/1999
10/26/1999
10/26/1999
10/26/1999
10/26/1999
10/26/1999
Dilution
Factor









1
1
1
1
1
1
1
1
1
1
QC Cone
Hg/L
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
Measured Cone
ug/L
0.95
0.94
0.99
0.97
1.01
0.97
0.92
0.94
0.94
0.96
0.98
0.96
0.95
0.94
0.95
0.97
1.01
0.99
1.02
Avg = 0.97
SD = 0.03
Range = 0.92 - 1 .02 (-8 to + 2%)
Date
3/26/1999
3/26/1999
3/26/1999
3/26/1999
3/26/1999
5/13/1999
5/13/1999
5/13/1999
5/13/1999
5/13/1999
5/13/1999
5/13/1999
6/15/1999
6/15/1999
6/15/1999
6/15/1999
6/15/1999
6/15/1999
6/15/1999
6/15/1999
Avg
SD
Range
Dilution
Factor
1
1
1
1
1











1
1
1
QC Cone
Hg/L
1.02
1.02
1.02
1.02
1.02
1.02
1.02
1.02
1.02
1.02
1.02
1.02
1.02
1.02
1.02
1.02
1.02
1.02
1.02
Measured Cone
Ug/L
0.90
0.93
0.84
0.86
0.86
0.95
0.99
0.97
0.97
0.99
1.02
1.06
1.03
1.07
0.98
0.96
0.98
0.98
1.00
1 1.02 0.98
= 0.97
= 0.06
= 0.84 - 1.07 (-16 to + 7%)
                                      74

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Table M-l. Results of QC Analysis (Contd)
Run#
E16.7-16.12
E16.7-16.12
E16.7-16.12
E16.7-16.12
E16.7-16.U
E17.7-17.12
E17.7-17.12
E17.7-17.12
E17.7-17.12
E17.7-17.12
E17.7-17.12
E17.25-17.2S
E17.25-17.28
E17.25-17.28
E17.25-17.28
E17.25-17.28
E17.25-17.28
E18.1-18.6
E18.1-18.6
E18.1-18.6
E18.1-18.6
Number = 21
Date
3/29/1999
3/29/1999
3/29/1999
3/29/1999
3/29/1999
5/15/1999
5/15/1999
5/15/1999
5/15/1999
5/15/1999
5/15/1999
6/2/1999
6/2/1999
6/2/1999
6/2/1999
6/2/1999
6/2/1999
6/11/1999
6/11/1999
6/11/1999
6/11/1999
Dilution
Factor

















1
1
1
1
QC Cone
Hg/L
2.96
2.96
2.96
2.96
2.96
2.96
2.96
2.96
2.96
2.96
2.96
2.96
2.96
2.96
2.96
2.96
2.96
2.96
2.96
2.96
2.96
Measured Cone
ug/L
2.88
3.04
2.92
3.14
3.00
2.89
2.89
2.98
3.01
2.98
3.01
2.97
2.97
2.92
2.94
3.01
2.92
2.92
2.90
2.97
2.95
Avg =2.96
SD = 0.06
Range =2.88 -3. 14 (-3 to + 6%)
                                      75

-------
Table M-l. Results of QC Analysis (Contd)
Run#
E1S.17-15.18
E15.17-15.18
E15.17-15.18
E15.17-15.18
E15.17-15.18
E15.17-15.18
E15.17-15.18
E15.17-15.18
E15.21-15.22
E15.21-15.22
E15.21-15.22
E15.21-15.22
E15.21-15.22
E15.21-15.22
E20.9
E20.9
E20.9
E20.9
E20.9
E20.23-20.30
E20.23-20.30
E20.23-20.30
E20.23-20.30
E20.23-20.30
E20.23-20.30
Number = 25
Date
9/27/1999
9/27/1999
9/27/1999
9/27/1999
9/27/1999
9/27/1999
9/27/1999
9/27/1999
9/11/1999
9/11/1999
9/11/1999
9/11/1999
9/11/1999
9/11/1999
10/7/1999
10/7/1999
10/7/1999
10/7/1999
10/7/1999
11/15/1999
11/15/1999
11/15/1999
11/15/1999
11/15/1999
11/15/1999
Dilution
Factor
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
QC Cone
ug/L
3.03
3.03
3.03
3.03
3.03
3.03
3.03
3.03
3.03
3.03
3.03
3.03
3.03
3.03
3.03
3.03
3.03
3.03
3.03
3.03
3.03
3.03
3.03
3.03
3.03
Measured Cone
Hg/L
2.90
3.03
3.06
3.06
2.97
2.99
2.95
3.06
2.97
3.07
3.09
2.92
2.96
2.94
2.94
3.05
2.90
2.92
2.94
2.90
2.92
2.97
2.95
3.00
3.09
Avg =2.98
SD = 0.06
Range = 2.90 - 3.09 (-2 to + 2%)
                                      76

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Table M-l. Results of QC Analysis (Contd)
Run#
E19. 19-19.24
E19. 19-19.24
E19. 19-19.24
E19. 19-19.24
E19.19-19.24
E19.19-19.24
E19.19-19.24
E19.19-19.24
E20.10-20.12
E20. 10-20.12
E20. 10-20. 12
E20.10-20.12
E21.4-21.10
E21.4-21.10
E21.4-21.10
E21.4-21.10
E22. 1-22.7
E22.1-22.7
E22. 1-22.7
E22. 1-22.7
E22. 1-22.7
Number = 21
Date
9/10/1999
9/10/1999
9/10/1999
9/10/1999
9/10/1999
9/10/1999
9/10/1999
9/10/1999
10/13/1999
10/13/1999
10/13/1999
10/13/1999
1/12/2000
1/12/2000
1/12/2000
1/12/2000
2/2/2000
2/2/2000
2/2/2000
2/2/2000
2/2/2000
Dilution
Factor
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
QC Cone
Hg/L
5.84
5.84
5.84
5.84
5.84
5.84
5.84
5.84
5.84
5.84
5.84
5.84
5.84
5.84
5.84
5.84
5.84
5.84
5.84
5.84
5.84
Measured Cone
Hg/L
5.83
5.90
5.83
5.78
5.85
5.94
5.99
5.71
5.68
5.77
5.71
5.75
5.49
5.67
5.80
5.73
5.82
5.89
5.82
5.88
5.93
Avg = 5.80
SD =0.11
Range = 5.49 - 5.99 (-6 to + 3%)
                                      77

-------
Table M-l. Results of QC Analysis (Contd)
Run#
E16.13-16.16
E16.13-16.16
E16.13-16.16
E16.13-16.16
E17.19-17.22
E17.19-17.22
El7.l9-n.22
E19.1-19.6
E19.1-19.6
E19.1-19.6
E19.1-19.6
E19.1-19.6
E19.1-19.6
E19.1-19.6
E19.1-19.6
Number =15
Date
3/31/1999
3/31/1999
3/31/1999
3/31/1999
5/26/1999
5/26/1999
5/26/1999
7/1/1999
7/1/1999
7/1/1999
7/1/1999
7/1/1999
7/1/1999
7/1/1999
7/1/1999
Dilution
Factor
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
QC Cone
ug/L
5.97
5.97
5.97
5.97
5.97
5.97
5.97
5.97
5.97
5.97
5.97
5.97
5.97
5.97
5.97
Measured Cone
ug/L
5.98
5.84
6.06
6.04
5.86
6.00
6.02
5.86
5.84
5.83
5.95
5.95
5.90
5.98
5.95
Avg , =5.94
SD = 0.08
Range = 5.83 - 6.06 (-2 to + 2%)
Run#
E19.24-19.30
E19.24-19.30
E19.24-19.30
E19.24-19.30
E19.24-19.30
E19.24-19.30
E19.24-19.30
E19.24-19.30
E20.13
E20.13
E20.13
E21.1-21.3
E21. 1-21.3
E21.1-21.3
E21. 1-21.3
Number = 15
Date
9/18/1999
9/18/1999
9/18/1999
9/18/1999
9/18/1999
9/18/1999
9/18/1999
9/18/1999
10/14/1999
10/14/1999
10/14/1999
12/30/1999
12/30/1999
12/30/1999
12/30/1999
Dilution
Factor
1
1
1












QC Cone
ug/L
9.88
9.88
9.88
9.88
9.88
9.88
9.88
9.88
9.88
9.88
9.88
9.88
9.88
9.88
9.88
Measured Cone
ug/L
9.26
9.42
9.69
9.66
9.49
9.51
9.53
9.55
9.36
9.57
9.50
9.55
9.75
9.95
9.75
Avg =9.57
SD =0.17
Range = 9.26 - 9.95 (-6 to + 1%)
                                      78

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Table M-l. Results of QC Analysis (Contd)
Run#
E15.13-15.16
E15.13-15.16
E15.13-15.16
E15.13-15.16
E17.23-17.24
E17.23-17.24
E17.23-17.24
E17.23-17.24
E19.7-19.12
E19.7-19.12
E19.7-19.12
E19.7-19.12
E19.7-19.12
E19.7-19.12
E19.7-19.12
E19.7-19.12
Number = 16
Date
3/20/1999
3/20/1999
3/20/1999
3/20/1999
6/1/1999
6/1/1999
6/1/1999
6/1/1999
7/6/1999
7/6/1999
7/6/1999
7/6/1999
7/6/1999
7/6/1999
7/6/1999
7/6/1999
Dilution
Factor
1
1
1
1
1
1
1
1
1





1
1
QC Cone
ug/L
10.29
10.29
10.29
10.29
10.29
10.29
10.29
10.29
10.29
10.29
10.29
10.29
10.29
10.29
10.29
10.29
Measured Cone
Hg/L
9.97
9.85
9.77
9.67
9.78
9.85
9.97
10.11
10.04
9.97
9.96
9.85
9.80
9.76
9.73
9.96
Avg = 9.88
SD = 0.12
Range = 9.67 - 10. 1 1 (-6 to -2%)
                                     79

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Table M-2. Results of WS Standards Analysis (WS 037)
Run#
E03-04
E05
E07-10
E07-10
El 1-12
E14
E15.1-15.6
E15.7-15.12
E1S.13-15.16
E15.17-15.18
E15.19-15.20
E15.21-15.22
E16.1-16.6
E16.7-16.12
E16.13-16.16
E17.1-17.6
E17.7-17.12
E17.19-17.22
E17.23-17.24
E17.25-17.28
E18.1-18.6
E18.7-18.8
E19.1-19.6
E19.7-19.12
E19.13-19.16
E19.19-19.24
E19.24-19.30
E20. 1-20.4
E20.5-20.8
E20.9
E20.10-20.12
E20.13
E20.14-20.22
E20.23-20.30
E21. 1-21.3
E21.4-21.10
E21.ll
E22. 1-22.7
Number = 38
Date
11/26/1998
1/27/1998
12/4/1998
12/4/1998
12/17/1998
2/22/1999
3/8/1999
3/16/1999
3/20/1999
9/27/1999
5/8/1999
9/11/1999
3/26/1999
3/29/1999
3/31/1999
5/13/1999
5/15/1999
5/26/1999
6/1/1999
6/2/1999
6/11/1999
6/15/1999
7/1/1999
7/6/1999
7/9/1999
9/10/1999
9/18/1999
9/25/1999
9/28/1999
10/7/1999
10/13/1999
10/14/1999
10/26/1999
11/15/1999
12/30/1999
1/12/2000
2/8/2000
2/2/2000
Dilution
Factor
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
EPA Cone
ug/L
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
49.3
Measured Cone
ug/L
46.31
46.35
48.56
49.51
49.30
49.96
47.80
47.01
48.99
47.21
47.28
47.16
46.23
47.94
47.47
46.80
48.76
47.33
48.08
46.84
46.38
47.98
50.10
46.25
47.27
48.41
49.09
49.19
49.00
49.25
48.34
48.83
45.01
48.50
50.13
48.87
48.94
50.39
Avg =48.1
SD = 1.3
Range = 45.0 - 50.4 (-9 to +2%)
                                       80

-------
Table M-2. Results of WS Standards Analysis (WS 038)
Run#
E07-10
E07-10
Ell-12
E14
E15.1-15.6
E15.7-15.12
E15.13-15.16
E15.17-15.18
E15.19-15.20
E15.21-15.22
E16.1-16.6
E16.7-16.12
E16.13-16.16
E17.1-17.6
E17.7-17.12
E17. 19-17.22
E17.23-17.24
E17.25-17.28
E18.1-18.6
E18.7-18.8
E19.1-19.6
E19.7-19.12
E19.13-19.16
E19.19-19.24
E19.24-19.30
E20.1-20.4
E20.5-20.8
E20.9
E20.10-20.12
E20.13
E20. 14-20.22
E20.23-20.30
E21. 1-21.3
E21.4-21.10
E21.ll
E22. 1-22.7
Number = 36
Date
12/4/1998
12/4/1998
12/17/1998
2/22/1998
3/8/1999
3/16/1999
3/20/1999
9/27/1999
5/8/1999
9/11/1999
3/26/1999
3/29/1999
3/31/1999
5/13/1999
5/15/1999
5/26/1999
6/1/1999
6/2/1999
6/11/1999
6/15/1999
7/1/1999
7/6/1999
7/9/1999
9/10/1999
9/18/1999
9/25/1999
9/28/1999
10/7/1999
10/13/1999
10/14/1999
10/26/1999
11/15/1999
12/30/1999
1/12/2000
2/8/2000
2/2/2000
Dilution
Factor
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
EPA Cone
ug/L
83.1
83.1
83.1
83.1
83.1
83.1
83.1
83.1
83.1
83.1
83.
83.
83.
83.
83.
83.
83.
83.1
83.1
83.1
83.1
83.1
83.1
83.1
83.1
83.1
83.1
83.1
83.1
83.1
83.1
83.1
83.1
83.1
83.1
83.1
Measured Cone
ug/L
79.44
80.38
73.50
77.59
76.67
72.97
76.19
74.29
76.94
74.76
73.80
75.05
74.66
73.77
75.33
75.30
76.70
73.88
74.73
75.67
77.85
73.69
75.70
77.51
76.89
76.48
77.12
75.95
78.03
74.66
74.41
76.74
76.32
75.49
78.29
77.82
Avg = 76.0
SD = 1.7
Range =73.0 -80.4 (-12 to -3%)
                                      81

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Table M-2. Results of WS Standards Analysis (WS 040)
Run#
E07-10
E07-10
El 1-12
E14
E15.1-15.6
E15.7-15.12
E15.13-15.16
E15.17-15.18
E15.19-15.20
E15.21-15.22
E16.1-16.6
E16.7-16.12
E16.13-16.16
E17.1-17.6
E17.7-17.12
E17.19-17.22
E17.23-17.24
E17.25-17.28
E18.1-18.6
E18.7-18.8
E19. 1-19.6
E19.7-19.12
E19.13-19.16
E19.19-19.24
E19.24-19.30
E20. 1-20.4
E20.5-20.8
E20.9
E20.10-20.12
E20.13
E20. 14-20.22
E20.23-20.30
E21.1-21.3
E21.4-21.10
E21.ll
E22. 1-22.7
Number = 36
Date
12/4/1998
12/4/1998
12/17/1998
2/22/1998
3/8/1999
3/16/1999
3/20/1999
9/27/1999
5/8/1999
9/11/1999
3/26/1999
3/29/1999
3/31/1999
5/13/1999
5/15/1999
5/26/1999
6/1/1999
6/2/1999
6/11/1999
6/15/1999
7/1/1999
7/6/1999
7/9/1999
9/10/1999
9/18/1999
9/25/1999
9/28/1999
10/7/1999
10/13/1999
10/14/1999
10/26/1999
11/15/1999
12/30/1999
1/12/2000
2/8/2000
2/2/2000
Dilution
Factor
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
EPA Cone
H&/L
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
Measured Cone
"g/L
107.48
108.66
103.27
101.13
90.99
105.36
99.44
95.47
95.71
94.56
96.34
97.35
103.58
98.84
105.19
101.27
99.13
95.51
103.07
98.44
98.80
97.09
99.13
101.21
97.30
105.25
98.60
97.03
100.99
97.22
94.45
99.51
99.11
96.26
98.74
99.01
Avg =99.5
SD =3.9
Range = 91.0 - 108.7 (-1 1 to +7%)
                                       82

-------
Table M-2. Results of WS Standards Analysis (WS 040) (Contd)
Run#
E15.17-15.18
E15.21-15.22
E19.7-19.12
E19.13-19.16
E19.19-19.24
E19.24-19.30
E20. 1-20.4
E20.5-20.8
E20.9
E20. 10-20. 12
E20.13
E20. 14-20.22
E20.23-20.30
E21.1-21.3
E21.4-21.10
E21.ll
E22. 1-22.7
Number = 17
Date
9/27/1999
9/11/1999
7/6/1999
7/9/1999
9/10/1999
9/18/1999
9/25/1999
9/28/1999
10/7/1999
10/13/1999
10/14/1999
10/26/1999
11/15/1999
12/30/1999
1/12/2000
2/8/2000
2/2/2000
Dilution
Factor
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
EPA Cone
Hg/L
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
102
Measured Cone
Hg/L
96.27
96.36
97.43
99.02
100.65
98.54
104.23
99.08
98.84
100.82
95.19
95.02
100.77
100.61
96.88
100.37
99.01
Avg = 98.8
SD =2.4
Range = 95.0 - 1 04.2 (-7 to +2%)
                                      83

-------
Table M-2. Results of WS Standards Analysis (WS 041)
Run#
E07-10
E07-10
Ell-12
E14
E15.1-15.6
E15.7-15.12
E15.13-15.16
E15.17-15.18
E15.19-15.20
E15.21-15.22
E16.1-16.6
E16.7-16.12
E16.13-16.16
E17.1-17.6
E17.7-17.12
E17.19-17.22
E17.23-17.24
E17.25-17.28
E18.1-18.6
E18.7-18.8
E19.1-19.6
E19.7-19.12
E19.13-19.16
E19.19-19.24
E19.24-19.30
E20. 1-20.4
E20.5-20.8
E20.9
E20.10-20.12
E20.13
E20.14-20.22
E20.23-20.30
£21.1-21.3
E21.4-21.10
E21.ll
E22. 1-22.7
Number = 35
Date
12/4/1998
12/4/1998
12/17/1998
2/22/1998
3/8/1999
3/16/1999
3/20/1999
9/27/1999
5/8/1999
9/11/1999
3/26/1999
3/29/1999
3/31/1999
5/13/1999
5/15/1999
5/26/1999
6/1/1999
6/2/1999
6/11/1999
6/15/1999
7/1/1999
7/6/1999
7/9/1999
9/10/1999
9/18/1999
9/25/1999
9/28/1999
10/7/1999
10/13/1999
10/14/1999
10/26/1999
11/15/1999
12/30/1999
1/12/2000
2/8/2000
2/2/2000
Dilution
Factor
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
EPA Cone
ug/L
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
65.6
Measured Cone
ug/L
66.24
67.18
63.26
63.27
63.27
64.47
65.56
61.58
61.40
60.03
59.58
64.49
64.73
66.73
64.63
63.99
63.94
59.75
66.06
63.75
63.21
63.67
59.68
63.22
63.90
66.96
61.72
62.17
62.32
63.38
60.36
63.56
63.40
62.94
64.06
63.39
Avg = 63.4
SD = 2.0
Range = 59.6 - 67.2 (-9 to +2%)
                                       84

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Table M-3. Recoveries of Spiked WS Standards
Run#
E18.1-18.6
E18.7-18.8
E19.1-19.6
E19.7-19.12
E19.13-19.16
E19.19-19.24
E19.24-19.30
E20.1-20.4
E20.5-20.8
E20.9
E20. 10-20. 12
E20.13
E20. 14-20.22
E20.23-20.30
E21. 1-21.3
E21.4-21.10
E21.ll
E22. 1-22.7
E14
E15.1-15.6
E15.7-15.12
E15.13-15.16
E15.17-15.18
E15.19-15.20
E15.21-15.22
E16.1-16.6
E16.7-16.12
E16.13-16.16
E17.1-17.6
E17.7-17.12
E17.19-17.22
E17.23-17.24
E17.25-17.28
Date
6/11/1999
6/15/1999
7/1/1999
7/6/1999
7/9/1999
9/10/1999
9/18/1999
9/25/1999
9/28/1999
10/7/1999
10/13/1999
10/14/1999
10/26/1999
11/15/1999
12/30/1999
1/12/2000
2/8/2000
2/2/2000
2/22/1999
3/8/1999
3/16/1999
3/20/1999
9/27/1999
5/8/1999
9/11/1999
3/26/1999
3/29/1999
3/31/1999
5/13/1999
5/15/1999
5/26/1999
6/1/1999
6/2/1999
Sample
ID
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
WS037
Dilution
Factor
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
Unspiked Cone
Hg/L
4.63
4.79
5.00
4.62
4.72
4.84
4.90
4.91
4.90
4.92
4.83
4.82
4.50
4.85
5.01
4.88
4.89
5.03
4.99
4.93
4.70
4.89
4.72
4.72
4.71
4.62
4.79
4.74
4.69
4.87
4.77
4.80
4.68
Spiked Cone
Hg/L
6.72
6.87
6.95
6.79
6.83
6.91
6.84
6.92
6.83
6.86
6.78
6.86
6.57
6.87
7.00
6.79
6.96
6.78
6.65
6.93
6.86
7.00
6.58
6.87
6.76
6.59
6.71
6.84
6.78
6.86
6.93
7.07
6.92
%
Recovery
104.2
103.4
97.1
108.2
105.1
103.7
96.6
99.9
96.7
96.9
97.5
101.5
103.5
101.0
99.6
95.2
103.2
87.3
82.9
100.0
108.0
105.1
93.0
106.9
102.4
98.5
95.9
104.7
103.9
99.2
108.1
113.1
111.6

E18.1-18.6
E18.7-18.8
E19.1-19.6
E19.7-19.12
E19.13-19.16
E19.19-19.24
E19.24-19.30
E20. 1-20.4
E20.5-20.8
E20.9
E20.10-20.12
E20.13
E20. 14-20.22
E20.23-20.30
E21. 1-21.3
E21.4-21.10
E21.ll
E22. 1-22.7
6/11/1999
6/15/1999
7/1/1999
7/6/1999
7/9/1999
9/10/1999
9/18/1999
9/25/1999
9/28/1999
10/7/1999
10/13/1999
10/14/1999
10/26/1999
11/15/1999
12/30/1999
1/12/2000
2/8/2000
2/2/2000
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
7.47
7.56
7.78
7.36
7.57
7.75
7.68
7.64
7.71
7.59
7.80
7.46
7.41
7.67
7.63
7.54
7.82
7.78
9.52
9.31
9.69
9.26
9.53
9.75
9.54
9.84
9.71
9.72
9.70
9.54
9.30
9.72
9.51
9.58
9.89
9.68
102.5
87.6
95.4
95.0
98.2
100.2
93.0
109.9
100.3
106.3
94.9
104.1
94.6
102.7
94.2
101.7
103.2
95.3
                                      85

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E14
E15.1-15.6
E1S.7-15.12
E15.13-15.16
E15.17-15.18
E15.19-15.20
E15.21-15.22
E16.1-16.6
E16.7-16.12
E16.13-16.16
E17.1-17.6
E17.7-17.12
E17.19-17.22
E17.23-17.24
E17.25-17.28
2/22/1998
3/8/1999,
3/16/1999
3/20/1999
9/27/1999
5/8/1999
9/11/1999
3/26/1999
3/29/1999
3/31/1999
5/13/1999
5/15/1999
5/26/1999
6/1/1999
6/2/1999
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
WS038
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
7.75
7.49
7.30
7.61
7.42
7.69
7.47
7.38
7.50
7.46
7.37
7.53
7.52
7.66
7.38
9.47
9.49
9.30
9.74
9.28
9.44
9.47
9.48
9.47
9.68
9.60
9.58
9.75
9.75
9.30
86.0
100.0
100.0
106.3
93.0
87.8
99.9
105.0
98.3
111.1
111.6
102.7
111.0
104.4
95.9

E20.1-20.4
E20.5-20.8
E20.9
E20.10-20.12
E20.13
E20.14-20.22
E20.23-20.30
E21. 1-21.3
E21.4-21.10
E21.ll
E22. 1-22.7
9/25/1999
9/28/1999
10/7/1999
10/13/1999
10/14/1999
10/26/1999
11/15/1999
12/30/1999
1/12/2000
2/8/2000
2/2/2000
WS040
WS040
WS040
WS040
WS040
WS040
WS040
WS040
WS040
WS040
WS040
20
20
20
20
20
20
20
20
20
20
20
5.21
4.95
4.94
5.04
4.75
4.75
5.03
5.03
4.84
5.01
4.95

E18.1-18.6
E18.7-18.8
E19.1-19.6
E19.7-19.12
E19.13-19.16
E19.19-19.24
E19.24-19.30
E20. 1-20.4
E20.5-20.8
E20.9
E20.10-20.12
E20.13
E20.14-20.22
E20.23-20.30
E21.1-21.3
E21.4-21.10
E21.ll
E22.1-22.7
E14
E15.1-15.6
E15.7-15.12
E15.13-15.16
E15.17-15.18
E15.19-15.20
E15.21-15.22
E16.1-16.6
E16.7-16.12
E16.13-16.16
6/11/1999
6/15/1999
7/1/1999
7/6/1999
7/9/1999
9/10/1999
9/18/1999
9/25/1999
9/28/1999
10/7/1999
10/13/1999
10/14/1999
10/26/1999
11/15/1999
12/30/1999
1/12/2000
2/8/2000
2/2/2000
2/22/1998
3/8/1999
3/16/1999
3/20/1999
9/27/1999
5/8/1999
9/11/1999
3/26/1999
3/29/1999
3/31/1999
WS041
WS041
WS041
WS 041 -
WS041
WS041
WS041
WS041
WS041
WS041
WS041
WS041
WS041
WS041
WS041
WS041
WS041
WS041
WS041
WS041
WS041
WS041
WS041
WS041
WS041
WS041
WS041
WS041
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
6.60
6.37
6.32
6.36
5.96
6.32
6.38
6.69
6.17
6.21
6.23
6.33
6.03
6.35
6.34
6.29
6.40
6.33
6.32
6.56
6.45
6.55
6.15
6.14
6.00
5.96
6.44
6.47
7.26
7.08
6.99
7.04
6.78
6.80
7.00
7.11
6.94
7.11
7.06

8.47
8.46
8.17
8.17
8.05
8.37
8.53
8.83
8.35
8.22
8.28
8.36
7.94
8.25
8.27
8.17
8.39
8.24
8.20
8.56
8.22
8.78
8.03
8.17
8.08
8.01
8.53
8.67
102.6
106.5
102.9
100.1
101.5
102.6
98.4
104.1
105.0
104.9
105.9

93.4
104.2
92.8
• 90.5
104.3
102.8
107.4
107.2
109.2
100.3
102.7
101.5
95.5
95.0
96.9
94.1
99.6
95.3
94.2
100.0
88.5
111.3
93.9
101.9
104.1
102.5
104.3
110.1
86

-------
E17.1-17.6
E17.7-17.12
E17.19-17.22
E17.23-17.24
E17.25-17.28
Number =110
5/13/1999
5/15/1999
5/26/1999
6/1/1999
6/2/1999
WS041
WS041
WS041
WS041
WS041
10
10
10
10
10
6.67
6.46
6.39
6.39
5.97
8.57
8.60
8.34
8.48
7.87
95.3
107.0
97.2
104.4
95.1
Avg = 100.6
SD =6.1
Range =82.9-113.1
87

-------

-------

-------
United States
Environmental Protection Agency/ORD
National Risk Management
   Research Laboratory
Cincinnati, OH 45268
Please make all necessary changes on the below label,
detach or copy, and return to the address in the upper
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detach, or copy this cover, and return to the address in the
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PRESORTED STANDARD
 POSTAGE & FEES PAID
          EPA
    PERMIT No. G-35
Official Business
Penalty for Private Use
$300
EPA/600/R-01/021

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