United States
                  Environmental Protection
                  Agency
 Water Engineering
 Research Laboratory
 Cincinnati OH 45268
                  Research and Development
 EPA/600/S2-86/070 Jan 1987
&EPA         Project Summary
                  Assessment  of  Assay  Methods
                  for  Evaluating Asbestos
                  Abatement  Technology  at the
                  Corvallis  Environmental
                  Research  Laboratory

                  Mark A. Karaffa, Robert S. Amick, Ann Crone, and Charles Zimmer
                   Two analytical methods and two
                  sampling techniques were evaluated
                  for their effectiveness in a project to re-
                  move air-entrainable asbestos from the
                  Corvallis Environmental Research Lab-
                  oratory in Corvallis, Oregon. The two
                  analytical methods were phase con-
                  trast microscopy (PCM) and transmis-
                  sion electron microscopy (TEM). The
                  sampling techniques included a static
                  (nonaggressive) method and an aggres-
                  sive one using a blower.
                   Air sampling was conducted at an
                  EPA office building that had undergone
                  an amosite asbestos abatement pro-
                  gram. The aggressive sampling tech-
                  nique revealed that air-entrainable as-
                  bestos  remained in work areas after
                  completion of abatement actions.  Re-
                  sults also confirm that under similar
                  sampling conditions, TEM analysis de-
                  tects more fibers than PCM because of
                  the former's better resolving capability.
                  Because PCM does not discriminate be-
                  tween asbestos and other fibers and
                  cannot resolve fibers  thinner than
                  about 0.2 ixm, this method may not ac-
                  curately reflect the true hazard poten-
                  tial.
                   TEM coupled with aggressive sam-
                  pling should  be recommended as the
                  analytical method of choice for final
                  post-abatement clearance testing.
                   This Project Summary was devel-
                  oped by EPA's Water Engineering Re-
                  search Laboratory, Cincinnati, OH, to
                  announce key findings of the research
                  project that is fully documented in a
separate report of the same title (see
Project Report ordering information at
back).

Introduction

Background
  The Technical Assistance Program of
the Office of Pesticides and Toxic Sub-
stances of the U.S. Environmental Pro-
tection Agency (EPA) provides guidance
and information on the identification of
asbestos-containing materials in build-
ings and on the correction of potential
asbestos hazards. Four EPA Guidance
Documents contain much of the exist-
ing technical information about  as-
bestos in nonindustrial settings.1"4
These documents describe how to es-
tablish an asbestos identification and
control program, provide background
information and direction to school offi-
cials and building owners on exposure
assessment, and give instruction on
how to develop and implement an as-
bestos abatement program. The most
recent asbestos guidance from EPA not
only emphasizes recent experience and
new information on asbestos control
but also introduces and discusses crite-
ria for developing  an appropriate  as-
bestos control plan.
  Considerable scientific uncertainty
still surrounds the effectiveness of
specific abatement actions in reducing
the risk  of exposure to airborne as-
bestos. One critical concern among
those responsible for asbestos abate-

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ment is how clean the contractor leaves
a building  (or building area) after re-
moving  the asbestos material or after
completing work  that could have dis-
turbed an asbestos-containing material
(e.g., encapsulation, enclosure, or spe-
cial maintenance  operations). The two
criteria recommended by the EPA guid-
ance (1983)3 that was in effect at the out-
set of this study were visual inspection
of the worksite and air monitoring after
completion of the project. Visual inspec-
tion should detect incomplete removal,
damage caused by abatement activity,
and (most  important) the  presence of
debris  or  dust  left by inadequate
cleanup of the work area. Air monitor-
ing by the membrane  filter collection
technique  and  phase-contrast  micro-
scopic  (PCM)  analysis are recom-
mended to supplement the visual in-
spection and to  determine whether
elevated levels of  airborne fibers gener-
ated during the removal process have
been sufficiently reduced. This currently
recommended optical microscopic
technique is one of two methods speci-
fied by the National  Institute for Occu-
pational  Safety and Health (NIOSH) to
determine airborne fiber  concentra-
tions; it is used  by the Occupational
Safety  and  Health Administration
(OSHA) to measure total airborne fibers
in occupational environments.
   The  EPA-recommended  air-
monitoring methodology for determin-
ing abatement  completion (NIOSH
Method  No.  P&CAM 239) was  as fol-
lows:
   Air sampling should begin after
   the project has been completed
   and all surfaces in the abatement
   site have been  cleaned, preferably
   within 48  hours  after abatement
   work  is finished.  A minimum  of
   three air monitors  per  worksite
   and at least  one  per room is rec-
   ommended.  Air is drawn through
   a membrane  filter for about  8
   hours at a flow  rate of approxi-
   mately 2 L/min. A total air volume
   of  approximately 1,000 liters col-
   lected at the specified  flow  rate
   should be sampled. After the sam-
   pling, a section  of the filter is
   mounted on a microscope slide
   and treated to  form a transparent,
   optically homogeneous gel. The
   fibers are sized  and counted by
   using a phase-contrast micro-
   scope at 400 to  450X magnifica-
   tion.  For  counting purposes,  a
   fiber is defined as a particle with a
   physical dimension longer than 5
   micrometers and a length-to-
   diameter ratio of 3 to 1 or greater.3

This method is intended to give an in-
dex  of the airborne concentration  of
fibers of specified dimensions in an at-
mosphere known or suspected to con-
tain asbestos; it is not designed to count
fibers less than 5 jxm long or to differen-
tiate asbestos  fibers from other fibrous
particulates.
  The most significant limitation of the
PCM method compared with transmis-
sion electron  microscopy (TEM) and
scanning electron microscopy (SEM) is
that PCM  ts limited in the detection of
fine particles (i.e., those with submicron
diameters  or  lengths less than 5 (jum)
that may  be toxicologically significant.
For example, in glove-box tests of simu-
lated industrial mechanical operations
on  asbestos-containing  products
(drilling, sawing, and sanding), the PCM
method counted fewer than 1 percent of
the  fibers  counted  by TEM.5 Although
conditions of this glove box study were
obviously different from  asbestos
abatement activities, some concern ex-
isted about the relative merits and capa-
bilities of the  different analytical meth-
ods used  to determine representative
fiber concentrations. Another study es-
timated that small asbestos  fibers (i.e.,
fibers less than 0.2-jxm wide and  5-|xm
long that are  not detected by the PCM
method) were present at 50 to 100 times
the concentration of the larger, optically
visible fibers.6

Study Objective
   The objective of this research project
was to identify and quantify the air-
 borne amosite asbestos fibers  present
 in building atmospheres after an  as-
 bestos remedial activity was completed
 and the building was reoccupied. The
 project focused on the  adequacy of
 EPA's previously recommended PCM
 method of analysis and  static sample
 collection technique. The PCM  method
 was compared with TEM  methods, and
 the feasibility of an alternative aggres-
 sive sampling technique was  investi-
 gated. The results of this study estab-
 lished the advantages and limitations of
 applying PCM and TEM analytical meth-
 ods, both separately and in conjunction
 with an aggressive sampling technique,
 to the evaluation of air quality following
 asbestos abatement.
   Reliable methods of air sampling and
 analysis  permit the use of  monitoring
 results to be  included in evaluating the
 efficacy of asbestos abatement  meth-
ods and in developing better technical
guidance  for abatement contractors,
building owners, and other parties di-
rectly responsible for remedial asbestos
programs. Active or recently completed
abatement sites were selected for moni-
toring because they provided an excel-
lent  opportunity to collect real-world
data, and because the monitoring tasks
could be arranged with minimum lead
time and coordination.
  The  conditions in a work area while
the final air samples are collected can
greatly influence the results of a posta
batement assessment. After an abate
ment action, the air is usually sampled
under  static conditions—that  is while
the area is sealed off, before ventilation
is restored, and after at least a 24-hour
settling period  following the final wet
cleaning. Consequently, this monitoring
technique may fail to detect  residual
fibers  that  have  settled  on  horizontal
surfaces or that were missed by the
cleaning.
  Residual asbestos fibers constitute a
potential exposure hazard because they
could be reentrained later, when the air
in the area is agitated by personnel traf
fie, air flow from  ventilation systems,
and  custodial activities. Thus, for  more
accurate characterization of postabate-
ment fiber concentrations, the  work
area should experience appreciable air
movement to simulate actual use condi
tions during air monitoring.

  The introduction of air turbulence into
the  work area  during the collection of
stationary air samples is  termed
 "aggressive sampling." This method
entails the creation of air movement by
the  use  of blowers, fans, brooms, or
compressed air streams  to entrain any
 particulate matter that may be present.
The advantages of the aggressive sam-
 pling  technique  over the static  (or
 nonaggressive) sampling are that the
former reflects worst-case conditions
 and that the testing requires a relatively
 short  period.  The disadvantages are
that this technique is not readily stand-
 ardized or reproducible,  nor does it re-
 flect normal exposure levels  to  occu-
 pants. As  with  the  static sampling
 method,  no criteria have been estab-
 lished to define an acceptable or safe
 level of fibers in a nonoccupational en-
 vironment. The research on fiber con-
 centration  levels using  the  PCM and
 TEM methods  is continuing so that the
 before-, during-,  and after-abatement
 criteria can be developed within the
 next 2 years.

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Project Description

Site Selection
  Air monitoring was conducted at two
selected sites from which friable  as-
bestos building materials had been re-
moved:  Site 1, Columbus East High
School, Columbus, Indiana; and Site 2,
the EPA Environmental Research Labo-
ratory in Corvallis, Oregon.
  This report describes only the results
of the air monitoring survey conducted
at Site 2. The monitoring data from Site
1 and the significance of these data  are
the subject of a separate report (Assess-
ment of Assay Methods for Evaluating
Asbestos Abatement Technology:
Columbus East High School, Columbus,
Indiana,  EPA/600/2-86/053). These  se-
lected sites met the following criteria:
  • The abatement plan involved the re-
    moval of friable,  spray-applied,
    asbestos-containing material.
  • The contractors carried out the work
    area  preparation, removal, and  de-
    contamination in accordance with
    EPA-recommended specifications
    and requirements.1
  • Multiple  work areas containing  ho-
    mogeneous asbestos material were
    available for monitoring.
  • The building owner and abatement
    contractor agreed to cooperate with
    EPA  and to provide access to  se-
    lected areas of the building.

Building Description
  The Corvallis Environmental Re-
search Laboratory (CERL) is housed in a
two-story, reinforced-concrete structure
built in  1966. The building contains a
total gross area of approximately 465
m2 (45,000 ft2).  A single-pass heating,
ventilating, and air conditioning (HVAC)
system supplies the occupied building
areas with 100 percent outside air. The
outside  air enters through intakes  on
the roof, is tempered by heating or chill-
ing coils, and is distributed by a closed-
duct system to ceiling-mounted dif-
fusers in  all rooms and laboratories.  Air
flows through louvers in the bottom of
interior doors and passes into the hall-
ways (which  serve as air plenums to the
outside), or it is exhausted through lab-
oratory fume hoods.

Asbestos-containing Materials
  Asbestos-containing  insulation had
been  spray-applied and tamped on to
the concrete  ceiling (beams and deck) of
four rooms (Rooms  146, 155, 157, and
159) and the penthouse in the main
CERL facility  and on beams in the boiler
 room (Room  163). The large air intakes
 located under the building, which sup-
 ply ventilating air  to the boiler and
 chiller room, were also  lined with  as-
 bestos. The insulation material on  the
 ceilings of Rooms 155, 157, and 159 and
 in the air ducts had been removed in
 1984  during  a  controlled abatement
 program. The asbestos-containing insu-
 lation in Room 146 (deionizer room), the
 boiler room, and the penthouse was still
 in place.
  Samples collected from Room 146
 and the penthouse  were analyzed by
 polarized light microscopy and disper-
 sion staining. The results indicated 80
 percent amosite asbestos in each of the
 two bulk samples analyzed. At the time
 of the survey, the remaining insulation
 material was characterized as highly fri-
 able,  loosely packed,  and  showing
 some  signs  of deterioration (loose,
 hanging pieces were visible).

 Methods and Procedures
  The sampling procedures and analyti-
 cal methods are outlined briefly  here.
 They are described fully in the full  re-
 port and in the project summary for Site
 1 (Assessment of Assay Methods  for
 Evaluating  Asbestos Abatement Tech-
 nology: Columbus East High School,
 Columbus,  Indiana, EPA/600/2-86/053).

 Abatement Program
  The asbestos-containing insulation in
 Rooms 155, 157, and 159 and in the air
 intakes was removed between May 21
 and July 2, 1984. The abatement plan
 and schedule prepared by the contrac-
 tor and submitted to CERL were  re-
 viewed and approved by  EPA before
 work was begun. The work plan was in
 accordance with  the then-current EPA
 guidelines and EPA and OSHA asbestos
 regulations for asbestos removal and
 decontamination. On completion of the
 abatement  effort, CERL personnel sur-
 veyed the work performed by the abate-
 ment  contractor, performed additional
 cleaning of the work areas, and made
 arrangements for the painting of all ceil-
 ing surfaces from which the asbestos
 insulation had been removed.
  According to CERL accounts,  each
work area was isolated from the rest of
the building by temporary barriers. Ven-
tilation ducts  and openings to the out-
side or to adjacent rooms were sealed.
Walls and  floors  were  covered  with
 plastic sheeting. Fully protected abate-
ment workers first wetted the insulation
with amended water and then scraped it
off. The asbestos-containing debris was
placed in scalable plastic bags and dis-
posed of at a local EPA-approved sani-
tary  landfill. Each work area was
cleaned three times, and a settling pe-
riod of 24 hours was allowed between
cleanings. The ceiling surfaces were
painted to bond any residual fibers not
removed by the scraping, brushing, and
wet-cleaning.

Monitoring Approach
  The sampling strategy for this study
was to collect representative samples
for  PCM and TEM  analysis from
(1) rooms where friable asbestos-
containing  insulation had  been re
moved,  (2)  rooms that were  never
treated with  asbestos-containing mate-
rials, and (3) outdoors. Samples for sub-
sequent PCM and TEM analyses were
collected from two or three representa-
tive locations in each room approxi-
mately 6 weeks after completion of all
abatement activities. Two of the three
monitored rooms from which asbestos
insulation had been  removed had been
reoccupied. (Room  159 was  vacant at
the time of the survey.) Both static and
aggressive sampling techniques were
used in each room. The static sampling
was conducted first  during regular
working hours while the facility was oc-
cupied. The  aggressive sampling was
conducted on a Saturday when the sam-
pling areas  were  unoccupied. Filter
holders containing either 0.8-^m  Mil-
lipore* mixed-cellulose ester (PCM) or
0.4-p.m Nuclepore polycarbonate filters
(TEM) were positioned 1.3 to 1.5 m (4 to
5 ft) above the floor at arbitrary loca-
tions.  Battery-powered  sampling
pumps were used to draw air through
the filters. The constant-flow pumps
were calibrated to 2.5 L/min and were
operated for 8 hour per test. Samples
were collected concurrently at outdoor
locations during each monitoring pe-
riod.
  On completion of each  monitoring
survey, samples were  submitted to the
appropriate  laboratory for  preparation
and analysis. The Nuclepore filters were
carbon-coated before they were trans-
ported to the laboratory for TEM analy-
sis.

Overview  of Air  Sampling
Strategy
  Samples designated  for PCM  and
TEM analysis were collected with both
*Mention of trade names or commercial products
does not constitute endorsement or recommenda-
tion for use.

-------
static and aggressive methods in six dif-
ferent rooms. Samples were also col-
lected in the surrounding environment
outside the building. The areas sampled
included  three  rooms  that had been
treated previously with asbestos insula-
tion  and  have since been abated and
rooms that  have never been treated
with asbestos. Representative samples
for PCM  and TEM analysis were col-
lected approximately 6 weeks after all
abatement activities had been com-
pleted. Outdoor air samples  were col-
lected concurrently with indoor sam-
ples on the  roof of the building or at
ground level in the open field west of
the main building.

   Side-by-side samples (one for PCM,
one for TEM) were collected in each
room under static and aggressive sam-
pling conditions. The  number of sam-
ples per room was not specified by the
study design, but three of each type of
sample were collected within  each
room. The  static  sampling was  per-
formed first during regular working
hours with  the building  occupied and
the  HVAC system operating. The ag-
gressive sampling was performed on a
Saturday while the sampling areas were
unoccupied  and the HVAC system was
not  operating.  Placement of the sam-
pling equipment within each work area
was the same during both static and ag-
gressive sampling.


Results

Air Monitoring Results

   Table 1 presents a detailed listing of
the results of PCM and TEM analysis of
samples collected under aggressive and
static sampling conditions after abate-
ment. With  one exception, all concen-
trations  of  asbestos  fibers and total
structures under aggressive sampling
conditions were higher than the corre-
sponding measurements made under
static sampling conditions. The excep-
tion involved two  samples with differ-
ences that were considered negligible
 because they were below the limit of
 reliable quantitation for the analytical
 method.

   Comparisons of PCM and TEM analy-
 ses of samples collected under static
 and aggressive sampling conditions are
 presented graphically in Figure 1, which
 is based on the data results presented in
 Table 1.
Statistical Comparisons

Statistical  Method of Analysis
  The Mann-Whitney test was used to
determine whether the observed differ-
ences in  analytical methods and sam-
pling conditions  were statistically  sig-
nificant.7  Use of the Mann-Whitney test
required no prior assumption regarding
the nature of the  underlying probability
distribution function of measurements
of asbestos fiber concentrations.

Analytical  Methods
  Tables  2 and 3 compare the geometric
averages of  fiber concentrations deter-
mined by PCM and TEM analyses under
static and aggressive sampling condi-
tions. Table 4 summarizes these results.
Based on the application of the Mann-
Whitney  test and the assumption  that
the fiber/volume concentrations are
comparable, the difference between
PCM and TEM results is statistically sig-
nificant (i.e., p < 0.03) for samples col-
lected outdoors  and indoors in  abated
areas under static conditions. The dif-
ference between PCM and TEM results
from indoor sampling  of nonasbestos
areas was also  statistically significant
(p<0.10) under  static sampling condi-
tions;  however, this conclusion  was
based  on a small  sample size (n  = 3).
The  ratios of TEM/PCM concentrations
for static sampling were 3.0 for ambient
samples, 3.3 for indoor  abated-area
samples, and 7.5 for  indoor nonas-
bestos-area samples. The difference be-
tween PCM and TEM results is not
statistically  significant  (i.e.,  p > 0.005)
for indoor samples from  both  abated
and  nonasbestos areas under aggres-
sive sampling conditions. For aggres-
sive sampling in abated areas, the  ratio
of TEM/PCM was 1.8.  For aggressive
sampling in nonasbestos areas, the
ratio of TEM/PCM was  1.9.

Sampling Conditions
   Table 2 also compares static and ag-
gressive sampling conditions for  PCM
and  TEM analyses in both abated and
nonasbestos areas. The difference be-
tween the geometric average fiber con-
centrations  under static and aggressive
sampling conditions was statistically
significant (i.e., p < 0.002) for both  PCM
and TEM in abated areas. For  PCM
analyses, the ratio of aggressive to
static fiber concentrations was 7.0; for
TEM analyses,  the ratio was  3.7. For
sampling conducted  in  nonasbestos
areas, the difference between the geo-
metric average fiber concentrations
under static and aggressive sampling
conditions was statistically significant
for  PCM  analyses (i.e., p < 0.002) but
not statistically significant for  TEM
analyses (i.e., p > 0.04).  For nonas-
bestos areas, the ratio of aggressive to
static fiber  concentrations for  PCM
analyses was 8.0; for TEM analyses, the
ratio was 2.0.

Comparison of Indoor Abated
Samples and Ambient Samples
  Also included in Tables 2, 3, and 4 are
the PCM and TEM analyses of samples
collected in the  ambient atmosphere
and in indoor abated areas.
  The difference between asbestos con-
centrations measured under aggressive
sampling conditions in indoor abated
areas and those measured in ambient
samples was  statistically  significant
(p < 0.02). The ratio of asbestos concen-
trations measured by TEM under ag-
gressive sampling conditions in indoor
abated areas to ambient TEM concen
trations was 6.2.

Comparison of Indoor Nonas-
bestos Samples  and Ambient
Samples
  For samples analyzed by PCM, the ge-
ometric mean asbestos concentration
for indoor samples collected  statically
in nonasbestos areas was below the de-
tection limit of the analytical method, as
were the ambient PCM samples. Conse-
quently, no meaningful  comparisons
can  be  made. For  PCM  samples col-
lected aggressively,  the geometric
mean concentration  was 0.016 x 106
fibers/m3 compared with lower than
0.002 x  106 fibers/m3 for ambient sam-
ples, a ratio of 16.0 (if a concentration of
0.001 x  106 fibers/m3 for ambient sam
pies is assumed). This observed differ
ence was  statistically  significant
(p>0.01). One nonasbestos area
(Room 152, the instrumentation laboira
tory) was extremely dusty, so the ag-
gressive sampling procedure entrained
large quantities of house dust that had
accumulated on shelf and cabinet tops
over many  years.  This fact accounted
for the relatively high PCM fiber counts.
  For TEM  samples collected  inside
nonasbestos areas  under  static condi
tions, the  geometric  mean asbestos
fiber concentration was 0.015 x 106
fibers/m3 compared  with  0.006 x 106
fibers/m3 for TEM ambient samples, a
ratio of  2.5. This observed difference

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                                                                       T
                                                                                 T
                                                                                                   i  i
                                                               H.II.IIIIII....I Nonasbastos Areas
                                                                                Aggressive TEM
                   nun
                             Hum  Nonasbestos Areas
                                      Aggressive PCM
   MM..,.....,,..,,..,	 Nonasbestos Areas
                            Nonaggressive PCM
                                            [muni  Nonasbestos Areas
                                                     Nonaggressive TEM
                                            IM.MI
                                                   III,,,,Mill   Abated Areas
                                                              Aggressive TEM
  ••••••••••••<
                                          1MIHBIMI   Abated Areas
                                                     Aggressive PCM

                                       (l Abated Areas
                                       Nonaggressive TEM

                    iiiiini  Abated Areas
                        Nonaggressive PCM
±
 PCM
	l_
                             MM........  A™bJ*nt
                                         TEM
J	L
                                                                                                 Range
                                                         J	L
                                                                i  i  i  i
                                                                     _L
                                                                                              25%    75%
                                                                                               Percentiles
                                                                                           J	L
 .001
           .002  .003 .004 .005 .007  .010
                                             .020 .030 .040 .050 .070  .100


                                                     106 Fibers/m3
                                                                     200 .300 .400 .500 .700  1.00
                                                                                                      2.00
 Figure 1.    Comparison of airborne fiber concentrations for PCM and TEM under static (nonaggressive) and aggressive conditions.
was not statistically significant  (i.e.,
p>0.10), nor was it significant under
aggressive  sampling conditions  (i.e.,
p > 0.10), where the ratio was 5.0. Be-
cause the comparisons of fiber concen-
trations for TEM samples in nonas-
bestos areas and ambient samples are
based on very small sample sizes (n = 3
and  n = 2, respectively), the observed
differences  are not statistically signifi-
cant at a probability level of >0.05.

Comparison of Samples  From
Indoor Abated  and
Nonasbestos Areas
  For all PCM samples (aggressive and
static), the observed differences in fiber
concentrations in indoor abated  and
nonasbestos areas were not statistically
significant (p > 0.08 for static conditions
and p > 0.05 for aggressive conditions).
For PCM samples collected under static
conditions, the ratio of fiber concentra-
tions in abated areas  to nonasbestos
areas was  3.0  (a concentration  of
                             0.001 x 106 fibers/m3). For PCM sam-
                             ples collected under aggressive condi-
                             tions, the ratio was  1.3.
                               For all samples analyzed by TEM, the
                             difference between  abated and nonas-
                             bestos areas was also not statistically
                             significant (p  -0.10 for static condi-
                             tions). For TEM samples collected under
                             static conditions, the ratio of asbestos
                             fiber concentrations  in abated to nonas-
                             bestos areas was 0.7. Under aggressive
                             conditions, this ratio was 1.3 for as-
                             bestos structures, and 1.2 for asbestos
                             fiber concentrations.

                             Conclusions
                               The following conclusions  resulted
                             from this study:
                               1. The  aggressive sampling tech-
                                 nique used  in this  problem-
                                 definition study revealed that air-
                                 entrainable asbestos fibers were
                                 present in previously abated areas.
                                 TEM analysis of aggressive sam-
                                 ples from building areas that were
                                 never treated with asbestos insula-
                                                                       tion also revealed detectable levels
                                                                       of asbestos fibers.
                                                                       Regardless of the  analytical
                                                                       method used, the fiber concentra-
                                                                       tions measured under aggressive
                                                                       sampling conditions were signifi-
                                                                       cantly higher than those measured
                                                                       under static conditions. The ratios
                                                                       of aggressive to static PCM fiber
                                                                       concentrations in abated  and
                                                                       nonasbestos areas  were 7.0  and
                                                                       8.0,  respectively. By TEM analysis,
                                                                       these ratios were 3.7 and 2.0.
                                                                       The  study results clearly demon-
                                                                       strate that under similar sampling
                                                                       conditions, TEM analysis identifies
                                                                       more fibers than PCM.  The ratio of
                                                                       TEM/PCM fiber concentrations for
                                                                       static sampling  was 3.0 for ambi
                                                                       ent samples, 7.5 for  indoor nonas
                                                                       bestos  areas, and  3.3  for indoor
                                                                       abated samples. The ratios for ag-
                                                                       gressive sampling in indoor areas
                                                                       were about 2 to 1.
                                                                       Asbestos concentrations deter-
                                                                       mined by TEM in abated areas with

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Table  1.    Results of PCM and TEM Analysis

                                            Nonaggressive
Aggressive
PCM
Sampling Location
Abated areas
Room 155
Room 157
Room 159
Nonasbestos areas
Room 173
Room 152
Room 205
Outdoors (ambient)
Ground
Roof
Blanks
Sample
Number
COR-02
COR-03
COR-01
COR-04
COR-05
COR-06
COR-08
COR-07
COR-09
COR-13
COR-14
COR- 10
COR-16
COR- 19
COR- 11
COR- 17
COR-21
COR-44
COR-43
COR-18
COR-42
COR-45
COR-47
COR-48
COR-49
106 Fibers/m3
0.007 1
0.002*
< 0.002*
0.008*
0.006*
0.01*
<0.002#

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     aggressive sampling were signifi-
     cantly (6.2) times higher than am-
     bient  TEM  concentrations. The
     TEM concentrations under aggres-
     sive conditions in the nonasbestos
     areas were 5 times higherthan am-
     bient TEM concentrations, but this
     difference was not statistically sig-
     nificant.

Recommendations
  Although time-consuming and ex-
pensive, TEM should be recommended
as the analytical method of choice for
measuring airborne asbestos fiber con-
centrations for final clearance testing  of
work areas after  asbestos abatement.
After a standardized TEM protocol and
an aggressive sampling procedure are
incorporated into asbestos guidelines, a
criterion should be established to define
an acceptable asbestos fiber concentra-
tion  in  building areas  after asbestos
abatement.  Continued research should
focus on the development of a quicker,
less  expensive  method  for monitoring
buildings after asbestos abatement and
on more efficient abatement practices.
       References

       1.  U.S. Environmental  Protection
         Agency. Asbestos-Containing  Mate-
         rials in School Buildings: A Guidance
         Document,  Part  1. Office of  Toxic
         Substances,  Washington, D.C.
         20460. March 1979.
       2. Sawyer, R.N., and D.M.  Spooner.
         Asbestos Containing  Materials in
         School Buildings: A Guidance  Docu-
         ment,  Part 2. Office of Toxic Sub-
         stances, U.S. Environmental Protec-
         tion Agency, Washington, D.C.
         20460. March 1979.
       3. U.S. Environmental  Protection
         Agency. Guidance for Controlling
         Friable Asbestos-Containing Materi-
         als in  Buildings.  EPA/560/5-83-002,
         Office  of Toxic Substances,  Wash-
         ington,  D.C  20460. March 1983.
      4. U.S.  Environmental  Protection
         Agency. Guidance for  Controlling
         Asbestos-Containing Materials in
         Buildings. EPA/560/5-85-024,  Office
         of Toxic Substances, Washington,
         D.C. 20460. June 1985.
                      5.  Falgout, D. Environmental Release of
                         Asbestos  From Commercial Product
                         Shaping. EPA/600/2-85/044,
                         Engineering-Science, Fairfax, Vir-
                         ginia. August 1985.

                      6.  Chatfield,  E.J. Measurement of As-
                         bestos Fibre Concentrations in Ambi-
                         ent Atmospheres. Study No. 10, On-
                         tario Research Foundation. 1983.

                      7.  Mosteller, F , and R.E.K. Rourke.
                         Sturdy  Statistics: Nonparametrics
                         and Order Statistics,  Addison
                         Wesley, Reading, Massachusetts.
                         1973.

                       The full report was submitted in fulfill-
                      ment of Contract No. 68-03-3197 by PEI
                      Associates, Inc., under the sponsorship
                      of  the U.S.  Environmental  Protection
                      Agency.
 Table 3.   Comparison of Sampling Results by Sample Location*

                                     Sample Location Comparisons

Samples Included
in Comparison
PCM-Static
PCM-Aggressive
TEM-Static
TEM-Aggressive

Indoor Abated/
Indoor Nonasbestos
3.0
1.3
0.7 (0.7) +
1.2 (1.3)t

Indoor Abated
Outdoors
3.0
3.5
1.7(1.7)'
6.2 (6.7)*
Indoor
Nonasbestos/
Outdoors
1.0
16.0
2.5 (2.5) r
5.0 (5.2)'
*AII quantities are ratios of the geometric mean fiber concentrations. For PCM samples, fiber
 concentrations include all fibers greater than 5 (xm in length; for TEM samples, fiber concen-
 trations include all asbestos fibers.
* Ratio of geometric mean concentrations of asbestos structures.
Table 4.    Summary Comparison of PCM and TEM Analyses of Air Samples Collected During Static and Agressive Conditions*

                                                               Nonasbestos Areas                     Abated Areas

   Analytical Technique
PCM, fibers (>5\i.m)'m3


TEM, asbestos fibers/m3

TEM, asbestos structures/m3
Outdoor (Ambient)

      BDLf


     6,000§

     6,000§
Static
                                                                         Aggressive
                                  Static
                                                Aggressive
BDL

15,000
15,000
BLRQ*
(16,000)
30,000
31,000
BLRQ
(3,000)
10,000
10,000
BLRQ
(21,000)
37,000
40,000
*AII values are geometric means.
*BDL = Below detection limit ( = 1,136 fibers/m3).
*BLRQ = Below limit of reliable quantitation (^22,720 fibers/m-').
^Geometric mean based on two sample values. One sample value was below the detection limit for TEM analysis ( 5,688 asbestos fibers
 structures/m 3).

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       Mark A. Karaffa, Robert S. Amick, Ann Crone,  and Charles Zimmer are with
         PEI Associates, Inc., Cincinnati, OH45246-0100
       William C. Cain is the EPA Project Officer (see below).
       The complete report, entitled "Assessment of Assay Methods for Evaluating
         Asbestos Abatement Technology at  the Corvallis Environmental Research
         Laboratory," (Order No. PB 87-110 961 /AS, Cost $13.95, subject to change)
         will be available only from:
              National Technical Information Service
              5285 Port Royal Road
              Springfield, VA 22161
               Telephone: 703-487-4650
       The EPA Project Officer can be contacted at:
               Water Engineering Research Laboratory
              U.S. Environmental Protection Agency
              Cincinnati, OH 45268
United States
Environmental Protection
Agency
Center for Environmental Research
Information
Cincinnati OH 45268
Official Business
Penalty for Private Use $300
EPA/600/S2-86/070

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