United States
                   Environmental Protection
                   Agency
Risk Reduction
Engineering Laboratory
Cincinnati, OH 45268
                   Research and Development
EPA/600/S2-90/013 Aug. 1990
&EPA        Project  Summary
                   Treatability of RCRA Compounds
                   in  a BOD/Nitrification  Wastewater
                   Treatment  System with  Dual
                   Media Filtration

                   Steven I. Safferman and Sanjoy K. Bhattacharya
                    The discharge of nitrogen and poten-
                   tially toxic or hazardous organic com-
                   pounds to  the  environment from
                   wastewater treatment plants has come
                   under  scrutiny in recent years.  Of
                   specific concern are the many organic
                   compounds  excluded from Resource
                   Conservation and Recovery Act (RCRA)
                   regulations if they are discharged into a
                   wastewater treatment plant.  This study
                   investigated the treatability and fate of 28
                   organic RCRA compounds in a combined
                   organic removal and nitrification process
                   and secondary effluent gravity filtration.
                   A 3.8 L/min pilot-scale extended aeration
                   wastewater treatment system with dual
                   media  effluent filter was used  for the
                   study.  With a total concentration of ap-
                   proximately 1.5 mg/L organics (sum of
                   the concentration  of all 28 RCRA com-
                   pounds) in the aeration basin, most of the
                   compounds  were removed to  below
                   detectable limits by secondary treatment
                   under either acclimated or unacclimated
                   conditions. The effectiveness of the ef-
                   fluent  filter  to remove organic com-
                   pounds could not be assessed as most of
                   the compounds entering the filter were
                   already reduced  to below detectable
                   levels.   Ammonia removal was sig-
                   nificantly impaired at a total concentra-
                   tion of 19.2 mg/L organics in the aeration
                   basin. COD reduction was apparently not
                   inhibited at any of the spike concentra-
                   tions tested.
                    This Project Summary was developed
                   by EPA's Risk Reduction Engineering
                   Laboratory, Cincinnati, OH, to announce
                   key findings of the research project that
is fully documented In a separate report
of the same title (see Project Report or-
dering information at back).

Introduction
  The effects of discharging hazardous
wastes into the environment without ade-
quate treatment have become of increasing
concern to regulatory agencies and the
scientific community.  Some of the com-
pounds associated with hazardous waste
can accumulate in the environment and are
mutagenic, teratogenic, or carcinogenic.
They can also volatilize into the atmosphere,
cause ground water contamination, and in-
hibit wastewater treatment processes.
  Under the domestic sewage exclusion
statement,  certain RCRA-regulated com-
pounds are exempt from RCRA regulations
if discharged into wastewater treatment
plants. Such compounds are assumed to
be treated adequately by the facility and to
cause no process interference.
  The treatability of these compounds must
be assessed and their effect on the treatment
process understood. The specific objective
of this study was to investigate the treatability
and fate of selected organic RCRA com-
pounds in a combination BOD/nitrification
process. The importance of this objective is
realized when the recent changes in regula-
tions to require reduced ammonia discharge
are considered. Many wastewater treatment
plants that previously did not stabilize
nitrogen may now be required to do so.
  One common  means of achieving both
nitrification and carbonaceous  BOD
removal is with an extended aeration sys-

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 tern.  In this study, 28 RCRA compounds
 with a broad range of properties were spiked
 into a pilot-scale extended aeration system
 with dual media secondary effluent filters to
 achieve the following goals:

 • investigate the treatability and fate of the
   selected RCRA toxics in the nitrification
   process for both acclimated and  unac-
   climated conditions and

 • determine  the  effectiveness of effluent
   dual media filtration to remove the com-
   posite RCRA toxics.

 Procedure
   To carry out the objectives of this project,
 two 3.78 L/min pilot-scale extended aeration
 wastewater treatment plants with dual media
 secondary effluent filters were constructed
 and operated at  the  U.S.  Environmental
 Protection  Agency (EPA) Test and Evalua-
 tion Facility (T&E) in Cincinnati, Ohio. The
 hydraulic retention time of each system was
 16.8 hr and the average solids retention time
 was 21 days.
   The influent was primary effluent from the
 Mill  Creek  Wastewater Treatment Plant in
 Cincinnati,  Ohio; primary effluent from one
 of two pilot-scale conventional  activated
 sludge systems at the T&E facility; or a com-
 bination of both. Because the conventional
 pilot systems were being spiked with  the
 RCRA compounds,  this design allowed
 spiking of  the extended aeration systems
 continuously or intermittently at various
 toxics concentrations.
  The 28 RCRA compounds were spiked
 into the conventional pilot systems at several
 different concentrations ranging from 0.25 to
 1.0 mg/L of  each organic.  These  com-
 pounds were selected to ensure a  good
 representation of RCRA compounds with a
 wide range of properties.  The resulting ran-
 ges  were  Henry's coefficient: 0.0000 to
 0.944; and log octanol/water coefficient:
 0.07 to 8.69.
  The three  phased project  studied  (1)
 process  interference caused by organic
 RCRA compounds, (2) treatability and fate
 of organic RCRA compounds,  and  (3)
 removal of organic RCRA compounds by
 dual media effluent filtration. A description
 of the procedures for each phase follows.

PAiase 1,  Process Interference
  In  this phase, the effect of the 28 RCRA
compounds on the ability of a pilot-scale
extended  aeration wastewater treatment
plant to adequately treat the wastewater was
investigated.  Chemical  oxygen  demand
(COD), nitrate, ammonia, and total Kjeldahl
nitrogen (TKN) were the primary  process
control variables used  to evaluate the
system's performance.
   The performance was compared under
 five different spike conditions:

 • baseline, system performance before any
   spike had been added;

 • total  spike  concentration of 1.8 mg/L,
   which was  calculated  by summing the
   amount of each compound found from
   each event and taking the average of the
   three events;

 • total  spike  concentration of 4.8 mg/L,
   which were similarly calculated as above;

 • total spike concentration of 19.2 mg/L,
   which was calculated based on the spike
   pump's setting; and

 • discontinuation of spike.

 Phase 2, Treatability and Fate
   In this phase of the project, the concentra-
 tion of the 28 compounds at different stages
 of the treatment process were studied. The
 influent spike concentration was about 0.1
 mg/L for each compound, assuming mini-
 mal removal by  the primary clarifier. From
 the  literature and from preliminary opera-
 tions, this initial concentration was selected
 so as not to cause any process interference
 to the system.
  To investigate the removal and fate of the
 compounds under both acclimated and un-
 acclimated conditions, one of the extended
 aeration systems was spiked continuously
 with the RCRA  compounds to allow  the
 microorganisms  to become acclimated to
 the toxics.  The  other system was spiked
 only during  sampling events so that  the
 microorganisms would not become ac-
 climated.
  Data were obtained from three sampling
 events,  each consisting of a 48-hr time
 period. For the first 24 hr of each event, the
 unacclimated system was spiked with the
 RCRA compounds.   For each event,
 samples were  taken every 4 hr and com-
 posited for  analysis of the RCRA  com-
 pounds.

 Phase 3, Removal by Filtration
  In this phase, the efficiency of dual media
 effluent filtration to remove organic RCRA
 compounds was investigated.  To assess
the effectiveness, the concentration of each
 compound was measured in the secondary
effluent (before entering the filter) and then
 in the filter effluent.  Data were obtained for
two secondary effluents:

 • secondary effluent obtained from the ac-
  climated extended aeration system, which
  was being spiked at a total toxics con-
   centration into the aeration basin of 1
   mg/L and

 • secondary effluent obtained from the a>
   climated conventional system at the T&
   facility, which was being spiked at a tot
   toxics concentration into the aeratic
   basin of 4.8 mg/L.

   Samples were  collected for RCRA con
 pound analysis 11 times: 3 for the extende
 aeration system's secondary effluent and
 for the conventional secondary effluent.

 Results and Discussion

 Phase 1, Process Interference
   The process control parameters of CO[
 ammonia, and nitrate were used to gauc
 the process interference caused  by  th
 spiked RCRA compounds during Phase 1 c
 this study.  Four spike concentrations wer
 tested. Figure 1 shows the percent remov;
 of TKN and ammonia and the increase «
 nitrate resulting from secondary  treatmer
 for each spike concentration. The trends i
 the figure seem to indicate that nitrificatio
 was inhibited even at a low spike concentre
 tion, although ammonia reduction by secor
 dary treatment was not inhibited until spik
 concentrations between 4.8 and 19.2 mg/
 were  reached.  After  spiking  ceasec
 nitrification returned to its pre-spiking levi
 within  a  few days.  COD removal did  nc
 seem to  be adversely affected by the spik
 even at the highest composite concentratio
 tested, 19.2 mg/L
  To assess the interference of the RCR
 compounds on an unacclimated system,
 parallel system was intermittently spikec
 The average total  spike concentration (tot,
 concentration  of 28 compounds) was  1.
 mg/L for three  shock loadings, which ap
 parently caused no inhibition of COD or air
 monia  reduction.

 Phase 2, Treatability and Fate
  The treatability and fate of the 28 organi
 RCRA  compounds spiked in Phase 1 at
 total concentration of 1.8 mg/L was inves
 tigated in Phase 2 for acclimated and unac
 climated conditions. Composite samples c
 aeration  basin influent, secondary clarifie
 effluent, and recycled activated sludge wen
 collected three times for analysis of the com
 pounds.
  For the three sampling events  from th<
 acclimated system, all of the compounds
for which valid data were collected, wen
 removed to below detectable limits  (Tabk
 1).  The variations in the aeration  basin in
fluent were probably caused  by differen
 removal  rates of the compounds  in the
primary clarifier,  background concentra

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       700


        90


        80  -|


        70


        60  -


        50  -


        40  -


        30  ~i


        20  ~


        10  ~
                                             % Removal of ammonia caused by seconday treatment
% Removal of TKN caused by secondary treatment
                        Increase in nitrate caused by secondary treatment, mg/L
                 I     I      I     I     I      I     I     I     I      I     I
            0         2          4          6          8         10

                                                Total spike concentration, mg/L

 Figure 1.  Acclimated conditions, ammonia, TKN, nitrate vs. concentration of spike.
                                                             I
                                                            12
   ]
   14
I
16
I
18
Dns of the compounds in MSD's secondary
fluent and analytical errors.
 For the three sampling events for the un-
:climated system (spiked  intermittently at
i average total concentration of 1.3 mg/L),
ily four compounds had removals below
5%: 1,1,2-trichloroethane, bis(2-ethylhexyl)
ithalate, toluene, and nitrobenzene (Table
 . The unacclimated conditions apparently
d not result in a significant reduction in
imoval efficiency at the RCRA compound's
Dncentration tested.

hase 3, Removal by Filtration
 The secondary effluent from both the ac-
imated and unacclimated nitrification sys-
 ms were  passed through dual media
fluent filters for the  first 62 days of the
•oject.  The secondary  effluent from the
Dnventional system was passed through
ne of the filters after day  62.  Eleven
nalyses were run for the  organic RCRA
impounds going into and coming out of
ie filter: three for the nitrification secondary
fluent and the others for the conventional
Astern.
 The filters removed approximately 50% of
ie total suspended solids for most operat-
ig conditions. Because the secondary ef-
jent from the nitrification system had very
iw concentrations of RCRA compounds,
ie effectiveness of the filters could not be
                          assessed for the first 62 days of the project.
                          Higher concentrations of some of the com-
                          pounds were present in the secondary ef-
                          fluent from the conventional system.  The
                          results indicate acetone,  tetrahydrofuran,
                          tetrachloroethylene,  and  phenol had  sig-
                          nificant removals by the filters (Table 3).
                          Values for  these compounds, however,
                          were found only in Event 9. The remaining
                          compounds, for both Events 4 and 9,  had
                          removals under 20%.

                          Conclusions
                            The following conclusions were drawn
                          from this study:

                          • COD removal was not  inhibited at the
                            tested composite spike concentrations of
                            1.8, 4.8 (measured by summing  the con-
                            centrations of all 28 compounds into the
                            aeration basin), and 19.2 mg/L (estimated
                            based on pump setting).

                          • Ammonia removal was significantly in-
                            hibited at the composite spike concentra-
                            tion of 19.2 mg/L (the sum  of  the
                            concentrations  of all  28 compounds into
                            the aeration basin).

                          • Almost 100% of the spiked organic RCRA
                            compounds were removed when  the
                            spike concentration was at 1.8 mg/L  (the
  sum of the concentrations of all 28 com-
  pounds into the aeration basin) for the
  acclimated condition and 1.3 mg/L for the
  unacclimated condition.

• Adsorption did not appear to be a principal
  mechanism for the removal of the RCRA
  compounds.

Recommendations
  This study indicates that an extended
aeration wastewater treatment  plant can ef-
fectively remove low concentrations of or-
ganic RCRA compounds without interfering
with BOD, ammonia, and suspended solids
removal.   Because of the many different
combinations and concentrations of RCRA
compounds that could be  expected in the
influent to a municipal wastewater treatment
plant, a correlation between this study and a
real life situation is risky. Further studies and
surveys of actual  extended aeration was-
tewater treatment plants are required to con-
firm these study findings, to determine if or
when harmful  by-products are produced,
and  to find the  compound's removal
mechanism.
  This report was submitted in fulfillment of
Contract No. 68-03-4038 by the University of
Cincinnati under the sponsorship of the U.S.
Environmental Protection Agency.

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Table 1.  Summary of Sampling Events for Acclimated Conditions

                               Event 1                Event 2
                                                                           Event 3
Compound
Cyclohexanone
Methy ethyl ketone
Methyl isobutyl ketone
Tetrahydrofuran
Carbon tetrachloride
Chlorobenzene
Chloroform
1 , 2-Dichloroethane
1 , 2-Dichloropropane
Tetrachloroethylene
Trichloroethylene
1,1,1- Trichloroethane
1 ,1 ,2'Trichloroethane
Ethylbenzene
Toluene
o-xylene
Bis(2-ethylhexyl)phthalate
Butyl benzyl phthalate
1 , 4-Dichlorobenzene
2, 4-Dimethylphenol
2, 4-Dinitrophenol
Naphthalene
Nitrobenzene
4-Nitrophenol
Phenol
ABI.
210
130
120
130
79
190
100
110
99
73
69
100
91
75
130
150
40
N
N
10
40
64
35
N
120
% REDSEC
100
100
100
100
100
100
100
1
100
100
100
100
95
100
100
100
100
NF
NF
100
100
100
100
NF
100
ABI.
N
150
N
N
64
110
81
93
67
64
62
70
74
65
110
21
40
13
N
N
N
77
N
N
N
% REDSEC
NF
100
NF
NF
100
100
100
100
100
100
100
100
100
100
100
100
85
100
NF
NF
NF
100
NF
NF .
NF
ABI.
N
148
N
63
70
100
90
96
74
66
70
77
72
95
170
42
45
N
42
N
N
67
68
32
180
% REDSEC
NF
100
NF
100
100
100
100
100
100
100
100
100
100
100
100
100
100
NF
100
NF
NF
100
100
100
100
AVG. ABI.
ML)
210
143
120
97
71
133
90
95
80
68
67
82
79
78
137
71
42
13
42
10
40
69
52
32
150
AVG.
% REDSEC
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
100
#
1
3
1
2
3
3
3
2
3
3
3
3
3
3
3
3
3
1
1
1
1
3
2
1
2
       ABI.:  Concentration into aeration basin,
      AVG.:  Average.
          I:  Influent concentration less than effluent concentration.
         N:  None detected.
       NF:   Compound not found in aeration basin influent
 % REDSEC:  Percent of compound reduced by secondary treatment.
         #:  Number of samples with valid values, out of 3.

Note:  Acetone, Methylene Chloride, and Furfural were analyzed for but not found in feed or the compound was found in the blank.

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Table 2.  Summary of Sampling Events for Unacclimated Conditions

                               Event 1                Event 2
Event 3
Compound
Cyclohexanone
Methy ethyl ketone
Methyl isobutyl ketone
Tetrahydrofuran
Carbon tetrachloride
Chlorobenzene
Chloroform
1 ,2-Dichloroethane
1 ,2-Dichloropropane
Tetrachloroethylene
Trichloroethylene
1,1,1-Trichloroethane
1, 1 ,2-Trichloroethane
Ethylbenzene
Toluene
o-xylene
Bis(2-ethylhexyl)phthalate
1 ,4-Dichlorobenzene
Naphthalene
Nitrobenzene
Phenol
ABI.
180
140
71
97
36
65
72
N
50
19
27
64
61
17
57
48
38
28
54
76
170
% REDSEC
100
100
100
100
100
100
100
NF
100
100
100
100
100
100
67
100
100
100
100
75
100
ABI.
N
240
N
N
N
76
36
12
31
36
25
N
24
77
96
130
22
22
50
32
130
% REDSEC
NF
100
NF
I
NF
100
100
100
100
100
100
NF
88
99
99
98
82
100
100
78
100
ABI.
N
330
75
N
35
73
66
N
40
32
46
50
42
45
120
66 B
15
24
38
36
220
% REDSEC
NF
100
100
NF
100
100
100
NF
100
100
100
100
90
100
100
100
100
100
100
81
100
AVG. ABI.

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  Steven /. Safferman and Sanjoy K. Bhattacharya are with the Department of Civil and
       Environmental Engineering, University of Cincinnati, Cincinnati, OH 45221.
  Sidney A. Hannah is the Technical Project Monitor (see below)
  The complete report, entitled "Treatability of RCRA Compounds in a BOD I Nitrification
       Wastewater Treatment System with Dual Media Filtration" (Order No. PB 90-194
       7051 AS; Cost: $17.00, subject to change), will be available only from:
           National Technical Information Service
           5285 Port Royal Road
           Springfield, VA 22161
           Telephone: 703-487-4650
  The EPA Technical Project Monitor can be contacted at:
           Risk Reduction Engineering Laboratory
           U.S. Environmental Protection Agency
           Cincinnati, OH 45268
United States
Environmental Protection
Agency
Center for Environmental Research
Information
Cincinnati OH 45268
     BULK RATE
POSTAGE & FEES PAID
         EPA
   PERMIT No. G-35
Official Business
Penalty for Private Use $300
EPA/600/S2-90/013

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