United States
Environmental Protection
Agency
Environmental Research
Laboratory
Athens GA 30613-7799
Research and Development
EPA/600/S9-91/001  Apr. 1991
Project Summary

Biological  Remediation of
Contaminated  Sediments,  with
Special Emphasis  on  the Great
Lakes:  A Workshop  Report

Chad T. Jafvert and John E. Rogers (Editors)
  These proceedings describe a work-
 shop  held  July  17-19,  1990  In
 Manltowoc, Wl,  at which biological
 remediation of contaminated sediments
 was discussed. For the purpose of the
 workshop, contaminated sediments of
 primary interest were those within six
 of the Areas of Concern (AOC)  identi-
 fied by the U.S./Canada International
 Joint Commission's Great Lakes Water
 Quality Board; five of which are prior-
 ity concerns of the U.S. Environmental
 Protection Agency's Assessment and
 Remediation of  Contaminated Sedi-
 ments (ARCS) program.
   The workshop was organized around
 four topic areas: (1) Overview  of the
 Areas of Concern; (2)  Biological deg-
 radation of PCBs; (3) Biological degra-
 dation of PAHs; and (4) Biological
 treatment of metal species. For the first
 topic area, presentations were made
 describing site  characteristic  of the
 Ashtabula River,  OH; Buffalo River, NY;
 Sheboygan River, Wl;  Grand Calumet
 River, IN; Saginaw River and Bay, Ml;
 and Hamilton Harbor, Ontario, Canada.
 For the remaining topic areas, presen-
 tations were made by investigators ac-
 tively involved in either bench, pilot, or
 full-scale studies concerning these ar-
 eas. In this document extended ab-
 stracts written by the presenters are
 given, as well as brief summaries of
 the presentations and  discussion ses-
 sions.
   This Project Summary was developed
 by EPA's Environmental Research
 Laboratory, Athens, GA, to announce
 key findings  of the research project
 that Is fully documented In a separate
 report of the same title  (see Project
 Report ordering Information at back).

 Overview
  The current state-of-the-science of bio-
 logical remediation of contaminated sedi-
 ments was discussed in a workshop held
 July 17-19, 1990, in Manitowoc, Ml. Spe-
 cial emphasis was devoted to remediation
 alternatives for sediments within the Great
 Lakes Basin. The workshop was supported
 by the U.S. EPA's Great Lakes National
 Program Office, through the Assessment
 and Remediation of Contaminated Sedi-
 ments (ARCS) Program, by Environment
 Canada, and by  EPA's Biosystems Tech-
 nology Development Program.  More than
 60 scientists from state and federal agen-
 cies, academia, and the private sector from
 the United States,  Canada,  and  The
 Netherlands participated.
  For the purpose of the workshop, the
 sediments of primary interest were those
 within the Areas of Concern identified by
 the U.S./Canada  International Joint
 Committee's Great Lakes Water Quality
 Board. Most of the 42 Areas of Concern
 are located in  harbors,  bays, or river
 mouths; 25 are located within U.S. waters,
 12 within Canadian  waters, and 5 within
 international channels. Remedial Action
 Plans currently  are  being  developed for
 these areas under the 1987 revision  of
 the Great Lakes Water Quality Agreement.
 A major purpose of EPA's ARCS Program
 is to evaluate remediation alternatives for
 the cleanup of  these sites with special
 emphasis given to five sites. These five
 are Ashtabula River, OH;  Buffalo River,

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NY;  Sheboygan  River. Wl; Grand Calu-
met  River, IN; and Saginaw River  and
Bay, Ml.
  Presentations  were organized  around
four topic areas.

• Areas of Concern: Buffalo River Reme-
  dial  Action   Plan  Strategy  (J.C.
  McMahon), Reids Brook Superfund Site/
  Ashtabula River Area (P. Sanders), Coal
  Tar Contamination Near Rundle Reef,
  Hamilton Harbor (T. Murphy et al.)t Indi-
  ana Harbor/Grand Calumet River AOC
  (R. Brunner). Saginaw River/Bay AOC
  (G. Goudy), and Sheboygan River  and
  Harbor (B. L Eleder).

•  Polychtorinated Biphenyls: Aerobic Bio-
  degradation of PCBs  (R.  Unterman),
  Anaerobic    Dechlorination    and
  Bforemediation  of PCBs (J. F. Quensen
  et al.), Dechlorination and Biodegrada-
  tion of PCBs  (J. F. Quensen et al.),
  Dechlorination  and Biodegradation of
  Chlorinated Biphenyls in Anaerobic
  Sediments (G-Y.  Rhee and B.  Bush),
  PCB  Dechlorination in  the Sheboygan
  River (W. Cl Sonzogni), Anaerobic and
  Aerobic Biodegradation of Endogenous
  PCBs  (D.  A.  Abramowicz  and M. J.
  Brennan), and Remediation  Pilot Study
  in the Sheboygan River (D. S. Foster).

1  Polychtorinated Aromatic Hydrocarbons:
  Use of a  Mycobacterium  sp. in the
  Remediation of Polycyclic Aromatic Hy-
  drocarbons (C.  E.  Cerniglia), Fungal
  Degradation; of PAHs (J. Glasser), Re-
  cent Studies on the Microbial Degrada-
  tion  of PAHs and Their Relevance to
  Bioremediatipn  (J. Mueller), and Bio-
  logical Remediation of Contaminated
  Sediments  ifi The Netherlands (H. J.
  van Veen and G. J. Annokkee).

  Metals:  Bacterial Leaching  of Metals
 from -Various^Matrices Found»in-Sedk
  ments, Removing Inorganics from Sedi-
  ment  Associated  Waters  Using
  Bioaccumulation and/or Biofix Beads (P.
 Altringer and  S. Giddings), Biological
 Treatment of Metal-contaminated Water
 (H. Edenborn), Bioleaching of Ores (E.
 G. Baglin), [Mechanisms  of Bacterial
   Metals  Removal from Solids (A.  E.
   Torma and P. A, Pryfogle), and Linking
   Biological  and  Hydrogeochemicai
   Mechanisms of Sediment Leaching (R.
   H. Lambeth and B. C. Williams).

   Biological remediation to treat contami-
 nated sediments  may take several forms.
 Each form (or process design) has its own
 list of factors or parameters that  must be
 considered when optimizing treatment.
 Hence, there are generally no simple an-
 swers to questions  regarding the feasibil-
 ity of biological remediation alternatives.
 Sediments generally are not contaminated
 with single compounds  or even classes of
 compounds. Additionally, the interactions
 among the various organisms responsible
 for the decomposition of anthropogenic
• compounds~and the sediment matrix are
 unknown in many cases. The intent, then,
 of the Proceedings is to provide some
 conclusions that will benefit scientists and
 engineers who must make choices among
 diverse treatment technologies.

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Tha EPA Editors, Chad T. Jafvert (also the EPA Project Officer, see below) and John
  EL Rogers, are with the Environmental Research Laboratory, Athens, GA 30613-
  7799.                                             !
The complete report, entitled "Biological Remediation of Contaminated Sediments, with
  Special Emphasis on the Great Lakes: A Workshop Report," (Order No. PB 91-161
  673/AS; Cost: $15.00 subject to change)  will be available only from:
        National Technical Information Service
        5285 Port Royal Road
        Springfield, VA 22161
        Telephone: 703-487-4650
The EPA Project Officer can be contacted at:
        Environmental Research Laboratory
        U.S. Environmental Protection Agency
        Athens, GA 30613-7799
United States
Environmental Protection
Agency
Center for Environmental
Research Information
Cincinnati, OH 45268
      BULK RATE
POSTAGE & FEES PAID
         EPA
   PERMIT No. G-35
Official Business
Penalty for Private Use $300
EPA/600/S9-91/001

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