United States
                   Environmental Protection
                   Agency
Environmental Monitoring
Systems Laboratory
Las Vegas NV 89193-3478
                   Research and Development
EPA/620/SR-93/016    July 1994
w EPA      Project  Summary
                   Environmental  Monitoring  and
                   Assessment Program:  Arid
                   Ecosystems  1993
                   Implementation  Plan—Colorado
                   Plateau  Plot  Design Pilot Study
                   William G. Kepner
                     This implementation plan describes
                   the basis for activities planned during
                   1993 in the southeastern Utah portion
                   of the Colorado Plateau. Ecological in-
                   terpretation of information gathered on
                   monitoring sites requires plot configu-
                   rations that provide sufficient area! sup-
                   port to capture the  characteristics of
                   the biological communities. The study
                   objective and approach of the 1993 pi-
                   lot study are to determine the sampling
                   support area and optimum plot size for
                   selected indicator measurements (spec-
                   tral properties, vegetation composition
                   and abundance, and soil properties).
                   Plot size variance and configuration re-
                   lationships along with cost of data col-
                   lection in terms of available resources,
                   e.g., time and money, will be used for
                   optimal plot design evaluations.
                     This Project Summary was developed
                   by EPA's Environmental Monitoring
                   Systems Laboratory, Las Vegas, NV, to
                   announce key findings of the research
                   project that is fully documented in a
                   separate report of the same title (see
                   Project Report ordering information at
                   back).


                   Introduction
                     The U.S.  Environmental  Protection
                   Agency (EPA), in collaboration with other
                   federal agencies, states, research insti-
                   tutes, and university  systems, has initi-
                   ated the  Environmental Monitoring and
                   Assessment Program  (EMAP) to develop
                   a long-term approach  to assess and peri-
                   odically document the condition of eco-
                   logical resources at the regional and na-
                   tional scales and to  develop innovative
 methods for anticipating emerging prob-
 lems before they reach crisis proportions.
 The goals of EMAP are to monitor  and
 assess the condition of U.S. ecological
 resources and to contribute to decisions
 on environmental protection and manage-
 ment.
  To accomplish its goals and objectives,
 EMAP has established eight ecosystem
 monitoring and research groups (i.e., es-
 tuaries, coastal waters, Great Lakes,  sur-
 face waters, forests, agroeco.systems, arid
 ecosystems, and landscape ecology)  and
 seven cross-system program groups (i.e.,
 design and statistics, quality  assurance,
 information management, landscape char-
 acterization,  indicators, methods and lo-
 gistics, and  assessment and reporting).
 This implementation plan describes the
 basis for activities planned during 1993 by
 the EMAP arid ecosystems monitoring and
 research group.
  Arid ecosystems, as defined by EMAP,
 are terrestrial systems characterized by a
 climatic  regime  where potential evapo-
 transpiration exceeds precipitation, annual
 precipitation  ranges from <5 to 60  cm,
 and daily and seasonal temperatures range
 from -40 to 50°C. The vegetation in  arid
 ecosystems is dominated by woody pe-
 rennials, graminoids, succulents,  and
 drought-resistant trees.  Physiognomy is
 generally low-form and canopies typically
 open. Arid ecosystems include associated
 riparian communities; however, intensively
 managed agriculture, such  as  irrigated
 farmlands, is excluded even though it  may
 occur in the same climatic region.
  The EMAP Arid  Ecosystems include
• arid, semiarid, and subhumid regions of
 the United States and occupy much of the
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land surface area (excluding high-eleva-
tion  forests) west of 95°  W longitude.
EMAP-Arid is one of three EMAP Terres-
trial  Resource Groups that are  progres-
sively interacting  in activities  as  the pro-
gram matures.
  The objectives  of the EMAP Arid Eco-
systems resource monitoring and research
group (EMAP-Arid} parallel those estab-
lished for EMAP.  It is the intent and pur-
pose of EMAP-Arid to measure and report
on the extent, condition and trends of re-
source classes  (i.e., arid subpopulations)
within the  blogeographical provinces of
nearctte  and neotropical North  America
that occur within  arid, semiarid, and
subhumid climatic regimes. A number of
pilot and demonstration projects will  be
required  in the next few years to develop
indicators of the condition of arid ecosys-
tems and measurement procedures prior
to achieving full implementation.
  Environmental indicators are being de-
veloped to  collectively describe the condi-
tion of an arid ecosystem. Indicators "com-
mon" to  the EMAP Terrestrial Resource
Groups will be fostered by the EMAP-Arid
group. The operating strategy is model
based and identifies regional issues  and
assessment questions, links them with so-
cietal values that have biological relevance,
and  identifies indicators that, when mea-
sured and integrated, can evaluate the
status and trends in the condition of arid
ecosystems. Issues that have been identi-
fied  as  regionally important in arid eco-
systems are desertification, livestock graz-
ing,  biodiversity, water resource manage-
ment,  air  quality, and global  climatic
change.  Three societal values  are cur-
rently identified as significant to arid eco-
systems  and have served to focus the
conceptual development of the monitoring
and  research strategy for EMAP-Arid, es-
pecially relative to the selection  and use
of indicators. These values are

  •  Biological integrity—species compo-
     sition and structure (abundance and
     spatial arrangement) of biotic and abi-
     otic  elements and their associated
     functions  (ecological  processes at
     various levels (i.e., genetic,  species,
     population, community,  ecosystem,
     and  landscape) of  biological organi-
     zation.
  •  Aesthetics—broadly defined as  at-
     tributes that affect human perception
     and  appreciation of the environment.
  •  Productivity—the quantity and quality
     of ecological and nonconsumptive ser-
     vices  or products  provided by  arid
     resources and their capacity for long-
     term maintenance.
  The EMAP-Arid group has elected to
develop its first research indicators rela-
tive to productivity and biological integrity,
significant social concerns relating to the
critical issues of desertification 'and cli-
mate change in western North American
landscapes. Various measures, attributes,
and  indices are being evaluated as indi-
cators related to specific indicator catego-
ries. Three indicator categories of arid eco-
system condition (spectral properties, veg-
etation composition and abundance,  and
soil properties) were tested during the sum-
mer of  1992 in  the southeastern  Utah
portion of the Colorado Plateau. The 1993
activities will concentrate on the develop-
ment of optimal  field plot  sampling de-
signs from which to obtain measurements
for the  three indicator categories .evalu-
ated in the 1992 Pilot Study.

Study Objective and Approach
  The study  objective and approach of
the 1993 EMAP-Arid pilot study is to de-
termine the sampling support area  and
optimum plot size for selected indicator
measurements. Interpretation of ecologi-
cal condition at EMAP-Arid monitoring sites
requires an adequate  representation of
the vegetation and soils communities at a
site. A number of criteria have been used,
by researchers to determine the minimum
plot size and shape required to adequately
describe an area. The  measurement of
variables  for indicators on  EMAP-Arid
monitoring sites is intended to reflect the
status  of  plant  and soil  communities
present in the sampled resource commu-
nity. Thus, it is  important that the sam-
pling support area is of a sufficient size to
adequately characterize those indicator
variables for the plant and soil communi-
ties under consideration. The approach to
determining the EMAP-Arid indicator sam-
pling support area and plot  design will be
based to  some extent  on  the research
conducted by others. However, the EMAP-
Arid survey requirements for sampling plots
will  differ  from other studies in several
facets.  The sampling plots will  have to
serve as monitoring sites for a multiplicity
of measurements with  the potential for
repeated visits to the site over decades.
Complete  census of the plots may be nei-
ther feasible nor desirable under  these
circumstances. They must be set up  with
minimal disturbance to the  measurement
areas. The number, of plots or replicate
samples at the  site for monitoring  pur-
poses will be the number required for
specified confidence estimates in the sur-
vey. Thus, questions of  replicatipn  block-
ing with different treatments on the plots
within blocks as in experimental trials are
not relevant to the survey.
  The field  sampling  areas required for
this study will be coordinated and  inte-
grated by the three indicator groups. Large
macroplots estimated  to be larger  than
the indicator sampling support area will
be  established at each site and inten-
sively sampled.  Each  macroplot will be
selected so that it is in a single landscape
unit. These macroplots will be established
in three biomes (in parallel EMAP terms,
these are research types)—desertscrub,
woodland, and grassland. The macroplots
will be the basis for a uniformity sampling
trial at each site. Plots of varying configu-
rations can be crafted  from the uniformity
sampling trial on the macroplot to evalu-
ate their qualitative and quantitative prop-
erties. Each macroplot is subdivided into
a rectangular array of basic sampling units.
The area within a basic unit required for a
particular measurement will vary with the
type of indicator category measurement.
The measurement areas  may  be small
quadrats for vegetation cover measures,
a soil sample, or spectral reading area.
The measurement taken in these areas
will be sufficient  to represent the charac-
teristics of the basic  unit. The arrange-
ment of measurement areas,  relative to
one another within the basic units, will be
set up to accommodate sampling logistics
that are anticipated at a  monitoring site
for future surveys.
  The goal is to have a cost-efficient sam-
pling strategy that is sufficient to provide
an adequate description of the vegetation
and soils community at the site. The rela-
tionship between properties of indicator
parameters and  the plot size and shape
will be established and estimated for all
measurements most critical to the quanti-
fiable indicators  identified  for EMAP-Arid
monitoring. A final plot design operation-
ally  feasible for  indicator measurement
purposes in a future EMAP-Arid demon-
stration survey will be  determined  from
data  collected in  1993 on these large
macroplots.
  Study sites for 1993 were selected to
represent readily observable differences
in biome and productivity combinations;
however, every  possible  combination is
not represented because of time and cost
constraints. The desert scrub biome is the
most extensive biome in the Great Basin
biogeographic region. Low-, medium-, and
(medium)  high-producing  sites  were se-
lected to represent this major  biome. A
medium producing  grassland  and  high-
producing pinyon-juniper  site  were also
selected.  Low-producing  grassland  and

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pinyon-juniper sites are rare in the Great
Basin biogeographic province.

Macroplot Design
  Ecological  interpretation  of information
gathered on monitoring sites requires plot
configurations that provide sufficient areal
support to  capture the  characteristics of
the biological communities. The  general
1993 macroplot design to  determine  the
sample support area and  optimum plot
design is shown  in Figure 1. Plot size
variance and configuration relationships
along with cost of data collection in terms
of available  resources, e.g., time and
money, will be used for optimal plot  de-
sign evaluations. Optimal plot design  will
be determined empirically for all important
indicator  measurements via uniformity
sampling  trials with  nested plot designs
on three  EMAP-Arid formation types  or
biomes, i.e., desert scrub, grassland, and
conifer  woodland (pinyon-juniper). This
study needs to precede or  coincide with
indicator development before undertaking
projects at the survey demonstration level.
Macroplot sampling  areas are designed
for  vegetation, harvester ants, spectral
properties, and soils.

Indicators
  The EMAP  indicators are being devel-
oped as  characteristics of the environ-
ment that, when  measured, quantify  the
magnitude of stress,  habitat characteris-
tics, degree of exposure to stressors, or
the degree of ecological response to an
exposure. Indicators serve  as the basis
for quantification of the assessment end-
points  (i.e., the actual measurements to
be made). In the 1993 EMAP-Arid Colo-
rado Plateau  Plot Design Pilot Study, the
same three indicator categories and mea-
surements used in  the 1992 pilot will be
studied to determine the optimum plot size
for each. The  three indicator categories
are  vegetation composition and abun-
dance, spectral properties,  and soil prop-
erties.
   The  composition, structure, and abun-
dance of vegetation have been recognized,
                                          Soils/Vegetation/Spectral Macroplot Design
    Enter/Exit
   Veg/Spectral
      Here
             180M
                                                                                                   1. Trees/Veg Sampling
                                                                                                     Sequence
                                                        3. X = Basic Soil Unit
                                                          (36 Samples)

                                                          ®= Auger Samples
                                                            (9 Samples)
                                                        4. Tree Plot
                                                                                                        15m
                                                                                                      5m
                                                                                                               Grasses/Forbes
                                                                                                               (576 Readings)
                                                                                                   5.
                                                                                                        Spectral N Shrubs/Ants
                                                                                                   (1440 Readings)   (288 Readings)
                                                                                                   «—  Enter/Exit
                                                                                                        Veg/Spectral Here
               Figure 1. Macroplot design for soils, vegetation, and spectral properties indicators.

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as useful indicators of environmentally in-
duced  changes in  arid vegetation.  The
proposed measurements for the determi-
nation  of these indicators are estimation
of (1) the percent cover and (2) the height
of the green vegetation on  the site by
species.  Together  these  measurements
can  provide an index of leaf area  and
serve as sensitive indicators of change in
biological condition at the organism, popu-
lation, community, and ecosystem levels.
  Electromagnetic radiation provides in-
formation about the physical and chemi-
cal properties of materials. While the spec-
tral reflectance properties of objects tend
to be wavelength dependent, the determi-
nation  of these relationships is critical for
characterizing or discriminating the objects.
Vegetation, soils, and other materials have
spectral responses that are a function of a
diverse array of properties of those mate-
rials. These properties might include mois-
ture content, shadowing, and presence of
other materials. Nevertheless, the overall
spectral response of a material is largely
a function  of the material itself. Spectral
measurements will be made to determine
if vegetation condition and soil properties
can  be related to spectral signatures col-
lected  by satellite, hand-held spectrom-
eters, or both. Ground spectra will be col-
lected using a portable field spectrometer.
The 1993 Plot Design Pilot Study wilt fo-
cus on correlating the spectral reflectance
measured on the ground with that deter-
mined from various satellite platforms to
estimate vegetation and soils features.
  Selected  soil  properties will  be  mea-
sured in the field and lab as indicators of
soil erosion, productivity, and  moisture-
plant  growth indices. Local soil charac-
teristics and soil surface and subsurface
samples wpl be obtained using established
methods from the U.S. Soil Conservation
Service. Local soil characteristics will be
used to evaluate type of soil and to calcu-
late an erosion  index.  Surface physical
and chemical soil attributes are obtained
from  samples collected at the surface.
Surface soil properties are one of the first
attributes  to respond to natural and an-
thropogenic stress. Deep soil profile ob-
servations will be collected to classify the
soil. Most of the measured soil properties
provide baseline data that would only be
resampled if notable changes occurred in
other indicators. The data can then be
used for comparison extrapolation and in-
terpolation of long-term change.
  The following research questions will
be  assessed in addressing  the  1993
EMAP-Arid objective:
  t.What is the relationship between indi-
    cator measurement properties and the
    size  and shape of plot for  selected
    vegetation, soils, and spectral indica-
    tor measures?
  2. What are the costs associated with
    setting up and sampling basic units
    for measuring the  selected vegeta-
    tion,  soils, and spectral indicator cat-
    egories?
  3. What are the sizes and shapes of
    plots that maximize the amount of
    information per unit cost for the se-
    lected vegetation, soils,  and spectral
    indicator  measures?
  4. What are the effects of spatial corre-
    lation patterns, if they exist, on the
    choice of plot size and shape?
  5. How similar  are the variances and
    correlation patterns across EMAP-Arid
    subpopulation formation types?
  The U.S.  Environmental Protection
Agency (EPA), through its  Office of  Re-
search and Development (ORD), funded
and collaborated in the research described
here.  It has  been peer reviewed by the
Agency and approved as an  EPA publica-
tion. Mention  of trade names or commer-
cial products does not constitute endorse-
ment or recommendation for use.
                                                                       u.S. GOVERNMENT PRINTING OFFICE: 1994 - 550-M7/80279

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   The EPA author, William G. Kepner, is with the Environmental Monitoring Systems
    Laboratory, Las Vegas, NV 89193-3478.
   Daniel T. Heggem is the EPA Project Officer (see below).
   The complete report, entitled "Environmental Monitoring and Assessment Program:
    Arid'Ecosystems 1993 Implementation Plan—Colorado Plateau Plot Design Pilot
    Study," (Order No. PB94-165339; Cost: $19.50; subject to change) will be
    available only from:
          National Technical Information Service
          5285 Port Royal Road
          Springfield, VA 22161
          Telephone: 703-487-4650
   The EPA Project Officer can be contacted at:
          Environmental Monitoring Systems Laboratory
          U.S. Environmental Protection Agency
          Las Vegas, NV 89193-3478
United States
Environmental Protection Agency
Center for Environmental Research Information
Cincinnati, OH 45268

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