APPENDIX I
of the
ASSESSMENT AND CONTROL OF
BIOCONCENTRATABLE
CONTAMINANTS IN SURFACE WATERS:
FIELD EVALUATION OF RESIDUE
PREDICTION PROCEDURES
1993 Draft
U.S. Environmental Protection Agency
Office of Research and Development
Office of Water
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FIELD EVALUATION OF RESIDUE PREDICTION PROCEDURES
USED IN ERA'S GUIDANCE:
•ASSESSMENT AND CONTROL OF BIOCONCENTRATABLE CONTAMINANTS
iN SURFACE WATERS":
THE FIVE MILE CREEK STUDY
1993 DRAR FOR APPENDIX I
Lawrence P. Burkhard1
Barbara Riedel Sheedy2-3
Nelson A. Thomas1
'U.S. Environmental Protection Agency
Environmental Research Laboratory-Duluth
6201 Congdon Boulevard
Duluth, MN 55804
2AScl Corporation
6201 Congdon Boulevard
Duluth, MN 55804
3Current Address:
Computer Sciences Corporation, 394 South Lake Avenue, Duluth, MN 55802
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On March 29, 1991, the U.S. Environmental Protection Agency announced the
availability of the draft guidance document "Assessment and Control of
Bioconcentratable Contaminants in Surface Waters" for review and comment in a
Federal Register notice (56 FR 13150). This 1991 draft bioconcentration factor
guidance (the "draft BCF [guidance]") did not contain Appendix I, the field evaluation
studies of the residue prediction procedures.
This draft Appendix I contains two field evaluation reports: the Louisiana study
and the Five Mile Creek Study. The two draft reports contain summary tables of the
field data, such as in-stream concentrations of the chemicals, tissue residues, and
predicted vs. measured tissue concentrations. Each study is followed by an appendix
of individual or raw field data, which were included for comment and review. The final
BCF guidance will not include the two field data appendices, so the reviewer is
encouraged to keep these sections for future reference.
At this time EPA is not asking for additional comments on the entire contents of
the 1991 guidance document, since EPA requested comments on the draft BCF (56
FR 13150) and extended the comment period to July 26,1991 (56 FR 26411).
Comments on the draft BCF were taken into account when EPA applied its
methodology in the Great Lakes proposal. For instance, on page II-5 of the draft BCF,
EPA recommended use of BCF values calculated from the log P values preferentially
over measured BCF values. Commenters suggested that measured BAFs and BCFs
take precedence over calculated values, and EPA modified the BCF approach used in
the Great Lakes proposal (58 FR 20802) to reflect these comments. Finally, EPA will
evaluate comments received on the bioaccumulation methodology in the Water Quality
Guidance for the Great Lakes System before preparing the final BCF document.
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Common and Scientific Names of Some of the Organisms
Collected in Field Studies
Common Name
Scientific Name*
Sea Catfish (hardhead)
Gulf Menhaden
Blue Crab
Crayfish
Banana Fish (Lady Fish)
Marsh Killifish
Cockahoo (mummy chog)
Channel catfish
Butterfish (spot)
Sunfish
Atlantic Croaker
Striped Mullet
Fiddler Crab
Arius filus
Brevoortia patronus
Callinectes sapidus
Decaooda sp.
Elopes saurus
Fundulus confluentus
Fundulus heteroclitus
Ictalarus punctatusb
Leiostomus xanthurus
Lepomis sp.
Micropogan undulus
Mugil cephalus
Uca pugilator
Scientific names of fishes taken from: Common and Scientific Names of North
American Fishes. American Fisheries Society. 1970.
Spelled I. ouctatus [sic] in the report
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Foreword
Recent advances in environmental sciences, analytical chemistry, and
toxicology have permitted the development of a systematic and scientifically defensible
procedure for identifying, assessing, and controlling chemicals which form residues in
fish and/or shellfish. This procedure is descrbied in the guidance document
"Assessment and Control of Bioconcentratable Contaminants in Surface Waters" and
is applicable to nonpolar organic chemicals which bioconcentrate and/or
bioaccumulate in aquatic organisms.
Because the regulatory application of this procedure will direct regulatory
decisions on the control of pollutants, EPA has designed and implemented field
studies to establish the validity of the approach. These field validation studies are
designed to show that the procedures can reliably use effluent data to identify the
presence and quantify the concentration of bioconcentratable contaminants in
receiving water organisms. The reasonable demonstration of accurate predictions in
several situations will be considered to establish this correlation.
This report presents results of the first field study conducted on a freshwater
site to determine how well tissue residue concentrations can be predicted in field
discharge situations using the guidance residue prediction procedure. Further work on
the samples from this field site are planned and these efforts will examine a much
larger set of chemicals. A report on the field study for another site will be published
separately.
Disclaimer
This document has been reviewed in accordance with U.S. Environmental
Protection Agency policy and approved for publication. Mention of trade names or
commercial products does not constitute endorsement or recommendation for use.
ACKNOWLEDGEMENTS
Technical assistance was provided by Donald Mount, Correne Jenson, Phillip
Marquis, Christine Soderberg, Jennifer Johnson and Kevin Hogfeldt (AScI Corporation,
Duluth, MN), Mark Yancey, Dale Foster, Laura Hernon-Kenny, Terry Nobles, Steve
Summer, William Clement, Dennis Mclntyre (Battelle Laboratories-Columbus Division),
Mick DeGraeve, John Shuey, Dennis McCauley (Battelle Laboratories-Great Lakes
Division), and Michael Griffin (State of Alabama). The clerical support of Debra
Williams (AScI Corporation, Duluth, MN) is gratefully acknowledged.
Financial support for this study was provided by the US-EPA Office of
Wastewater Enforcement and Compliance, Permit Division.
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Contents
Page No.
Foreword 2
Disclaimer 2
Acknowledgements 2
Contents 3
Figures 5
Tables 6
Executive Summary 10
Introduction 11
1.1 Site Study Objective 11
1.2 Constraints 12
Site Selection and Description 13
2.1 Description of Five Mile Creek, Birmingham, Alabama 13
2.2 Screening of the Effluents 15
2.3 Selection of Target Chemicals 15
Methods 17
3.1 Site Study Plan 17
3.2 Estimation of Residues in Aquatic Organisms 17
3.2.1 Prediction of Bioconcentration Factors
for Aquatic Organisms 17
3.2.2 Prediction of Bioaccumulation Factors
for Aquatic Organisms 18
3.2.3 Prediction of Residues in Aquatic Organisms 18
3.2.4 Metabolism and Prediction of Residues 18
in Aquatic Organisms
3.3 Sampling Procedures 19
3.3.1 Field Sampling Procedures for Effluents 19
3.3.2 Field Sampling Procedures for Ambient
Grab Samples 19
3.3.3 Field Procedures for Measuring Stream Flows 19
3.3.4 Field Procedures for Sampling Indigenous Organisms 19
3.3.5 Field Procedures for the Caged Ictalarus puctatus
Exposures 20
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3.4 Analytical Procedures 21
3.4.1 Effluent Analysis Procedure 21
3.4.2 Weekly Effluent Composite Analysis 22
3.4.3 Ambient Water Samples Analysis 22
3.4.4 Tissue Analysis 23
Results and Discussion 25
4.1 Expected Tissue Residues Trends 25
4.2 Flow Data for Five Mile Creek and Effluents 25
4.3 In-Stream Effluent Concentrations 26
4.4 Analysis of the Weekly Effluent Composites for the Five Target
Chemicals 31
4.5 In-Stream Chemical Concentrations for the Five Target Chemicals 31
4.6 Indigenous Organisms 31
4.6.1 Tissue Data 32
4.6.2 Residue Trends 32
4.6.3 Significance Testing of Residues 43
4.6.4 Comparison of Predicted and Observed Residues 44
4.6.5 Comparison of Measured and Predicted Residues:
Error Analysis 48
4.7 Caged Organisms 52
4.8 Comparison of the Measured and Predicted Tissue Residues:
Unaddressed Variables 53
Summary 55
References 56
Appendix A: Study Data 57
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Figures
Page No.
Figure 2-1. Map of Sampling Stations for Aquatic Organisms,
Sediment, and Caged Organisms on Five Mile Creek. 14
Figure 4-1. Measured and Predicted Flows on Five Mile Creek. 27
Figure 4-2. Measured Discharge Flow for Coke Plant 1. 28
Figure 4-3. Measured Discharge Flow for Coke Plant 2. 29
Figure 4-4. In-Stream Effluent Concentration for Coke Plant 1. 34
Figure 4-5. In-Stream Effluent Concentration for Coke Plant 2. 35
Figure 4-6. In-Stream Concentration for Biphenyl. 36
Figure 4-7. In-Stream Concentration for Phenanthrene. 37
Figure 4-8. In-Stream Concentration for Anthracene. 38
Figure 4-9. In-Stream Concentration for Fluoranthene 39
Figure 4-10. In-Stream Concentration for Pyrene. 40
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Tables
Page No.
Table 4-1. Average Predicted Stream Flows, Average Discharge
Flows, and Average In-Stream Effluent Concentration
for Two Coking Plants on Five Mile Creek for
March 26, 1990 - April 26, 1990. 30
Table 4-2. Concentration of Target Chemicals in Weekly Effluent
Composite Samples from Two Coking Plants on Five
Mile Creek, Birmingham, Alabama. 33
Table 4-3. Grand Mean Daily In-Stream Concentration of the Five
Target Chemicals for Stations 2 and 3 for Five Mile Creek. 41
Table 4-4. Average Tissue Residues (ppb) in Two Species of Resident
Organisms for Five Mile Creek. 42
Table 4-5. Residue Prediction Parameters. 45
Table 4-6. Predicted and Measured Decapoda Tissue Concentrations
for Five Mile Creek. 46
Table 4-7. Predicted and Measured Lepomis sp. Tissue Concentrations
for Five Mile Creek. 47
Table 4-8. Confidence Limits for the Predicted Decapoda Tissue
Concentrations for Five Mile Creek. 49
Table 4-9. Confidence Limits for the Predicted Lepomis sp. Tissue
Concentrations for Five Mile Creek. 50
Table 4-10. Average Tissue Residues (ppb) in Caged Ictalarus puctatus
for Five Mile Creek. 52
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Appendix A Tables: Page No.
Table A-1. Bioconcentratable Chemicals Tentatively Identified Using
the Effluent Analytical Procedure: Coke Plant 1, Fraction 1. A-1
Table A-2. Bioconcentratable Chemicals Tentatively Identified Using
the Effluent Analytical Procedure: Coke Plant 1, Fraction 2. A-3
Table A-3. Bioconcentratable Chemicals Tentatively Identified Using
the Effluent Analytical Procedure: Coke Plant 2, Fraction 1. A-10
Table A-4. Bioconcentratable Chemicals Tentatively Identified Using
the Effluent Analytical Procedure: Coke Plant 2, Fraction 2. A-11
Table A-5. Bioconcentratable Chemicals Tentatively Identified Using
the Effluent Analytical Procedure: Coke Plant 2, Fraction 3. A-12
Table A-6. Measured and Predicted Stream Flows at Three
Locations on Five Mile Creek, Jefferson County,
Alabama. A-13
Table A-7. Regression Equations for Predicting Stream Flows
at Stations 1 and 3 and Predicted Stream Flows with
95% Confidence and Prediction Intervals for Selected
Flows for Stations 1 and 3 for Five Mile Creek. A-14
Table A-8. Daily Effluent Flow for Coke Plant 1 and Coke Plant 2. A-15
Table A-9. In-stream Effluent Concentrations for Discharges from
Coke Plants 1 and 2. A-16
Table A-10. Coke Plant 1 Weekly Effluent Composite for Target
Analysis on Biphenyl. A-17
Table A-11. Coke Plant 1 Weekly Effluent Composite for Target
Analysis on Phenanthrene. A-18
Table A-12. Coke Plant 1 Weekly Effluent Composite for Target
Analysis on Anthracene. A-19
Table A-13. Coke Plant 1 Weekly Effluent Composite for Target
Analysis on Fluoranthene. A-20
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Table A-14. Coke Plant 1 Weekly Effluent Composite for Target
Analysis on Pyrene. A-21
Table A-15. Coke Plant 2 Weekly Effluent Composite for Target
Analysis on Biphenyl. A-22
Table A-16. Coke Plant 2 Weekly Effluent Composite for Target
Analysis on Phenanthrene. A-23
Table A-17. Coke Plant 2 Weekly Effluent Composite for Target
Analysis on Anthracene. A-24
Table A-18. Coke Plant 2 Weekly Effluent Composite for Target
Analysis on Fluoranthene. A-25
Table A-19. Coke Plant 2 Weekly Effluent Composite for Target
Analysis on Pyrene. A-26
Table A-20. In-Stream Concentration of Biphenyl for Stations 2
and 3 on Five Mile Creek. A-27
Table A-21. In-Stream Concentration of Phenanthrene for Stations
2 and 3 on Five Mile Creek. A-28
Table A-22. In-Stream Concentration of Anthracene for Stations 2
and 3 on Five Mile Creek. A-29
Table A-23. In-Stream Concentration of Fluoranthene for Stations 2
and 3 on Five Mile Creek. A-30
Table A-24. In-Stream Concentration of Pyrene for Stations 2 and 3
on Five Mile Creek. A-31
Table A-25. Tissue Target Analysis for Decapoda From Four Stations
on Five Mile Creek, Jefferson County, Alabama. A-32
Table A-26. Tissue Target Analysis for Lepomis sp. From Four Stations
on Five Mile Creek, Jefferson County, Alabama. A-34
Table A-27. Blank Concentrations and Surrogate Recoveries for Tissue
Analysis. A-36
Table A-28. Percent Lipid Content in Tissues From Four Stations on
Five Mile Creek, Jefferson County, Alabama. A-37
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Table A-29. Tissue Target Analysis for Ictalarus puctatus From Caged
Exposures at Five Mile Creek, Jefferson County, Alabama. A-38
Table A-30. Ambient Water Samples for Target Chemical Analysis for
Five Mile Creek, Birmingham, Alabama. A-39
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Executive Summary
This report describes an investigation to determine how well tissue residue
concentrations can be predicted in field discharge situations using the EPA's residue
prediction procedure. This procedure is used in EPA's guidance document entitled
"Assessment and Control of Bioconcentratable Contaminants in Surface Waters" to
predict residues in receiving water organisms.
The study consisted of measuring and predicting tissue residue concentrations
for receiving water organisms for a segment of Five Mile Creek, Birmingham,
Alabama. Two point source discharges, both from coke manufacturing facilities, were
included in the field site and five chemicals were studied, i.e., biphenyl, phenanthrene,
anthracene, fluoranthene, and pyrene. Effluent composites, receiving water organisms
(Decapods and Lepomis sp.), and flow data were collected in April, 1990. Data from
these samples were used to predict receiving water tissue concentrations and then, to
evaluate the guidance residue prediction procedure.
This investigation demonstrated that tissue residues in field discharge situations
can be predicted within a factor of 3 for "non-metabolizable" chemicals using the
guidance residue prediction procedure. When metabolism is important, residues
predicted using the guidance procedure will be too large. In these cases, the
guidance document recommends the use of measured
bioconcentration/bioaccumulation factors which includes the effects of metabolism in
the residue prediction.
Results from this investigation and from another yet to be completed will
demonstrate the predictive ability of EPA's residue prediction procedures.
10
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INTRODUCTION
The Environmental Protection Agency has developed a guidance procedure,
"Assessment and Control of Bioconcentratable Chemicals in Surface Waters' (1), to
control bioconcentratable chemicals in effluents. This guidance consists of a number
of technical procedures that have been developed during the past several years. The
principle components of the guidance approach are: 1) analytical procedures for
detecting and identifying bioconcentratable chemicals in effluents or receiving water
organisms, 2) prediction of the bioconcentration factor (BCF) from the n-octanol water
partition coefficient (P) using quantitative structure activity relationships (QSAR), 3)
prediction of the bioaccumulation factor (BAF) from the chemical's BCF and log P, and
the trophic status of the organism of concern, 4) prediction of residues in aquatic
organisms using the BCF or BAF and concentration of the chemical in the receiving
water, and 5) calculation of allowable ambient water or tissue residue concentrations
for bioconcentratable chemicals based upon human consumption of contaminated fish
and shellfish. The guidance protocol combines these procedures to arrive at
discharge concentrations for bioconcentratable chemicals which will limit residues in
aquatic organisms used for human consumption.
The guidance approach provides two alternatives for assessing point source
discharges for bioconcentratable chemicals, the effluent and tissue alternatives
(component 1). With these alternatives, either effluent from a point source discharge
or indigenous receiving water organisms are analyzed. Results from the analytical
methods for the both alternatives are listings of bioconcentratable chemicals. These
results are evaluated further using components 2 through 5, to determine if
development of permit limits are needed for any of the identified bioconcentratable
chemicals.
With the tissue alternative, the analytical results provide information for the entire
receiving water since the aquatic organisms provide an integrated assessment of all
point and nonpoint sources of bioconcentratable chemicals. When an unallowable
tissue residue is found, additional chemical analyses are required to determine the
source(s) of the residue forming chemical to the receiving water. In contrast, with the
effluent alternative, point source discharges are examined individually. The inclusion
of both alternatives in the guidance provides greater flexibility and usefulness for the
guidance approach since neither alternative by itself is useful in all permitting
situations.
1.1 Site Study Objective
The objective of the site study was to determine how well tissue residue
concentrations can be predicted in field discharge situations using the guidance
procedures, i.e., components 2, 3, and 4.
11
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This validation effort was not designed to verify a) the accuracy of the allowable
tissue residues, b) the analytical procedures associated with the tissue alternative, c)
the prediction of residues where exposure is intermittent, d) the prediction of residues
where exposure is difficult to estimate, or e) the derivation of acceptable human
uptake levels.
1.2 Constraints
In order to predict residues in receiving water organisms, the concentration of
the chemicals in the receiving water must be known and these concentrations (in the
receiving water) must be relatively constant for a 20 to 40 day period. Without these
conditions, successful evaluation of the field data will be nearly impossible since the
indigenous organisms will never come to steady-state conditions with the receiving
water.
These characteristics, in general, are associated with sites which: a) have
reasonably simple hydrodynamics so that receiving water concentrations can be
determined and/or calculated, b) have short hydraulic resident times so that fate and
halflrfe considerations are minimized for the discharged chemicals, c) have effluent
discharges with relatively constant concentrations of bioconcentratable chemicals, and
d) have limited sources of the bioconcentratable chemicals under investigation.
12
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SITE SELECTION AND DESCRIPTION
This report details the validation study performed on Five Mile Creek,
Birmingham, Alabama in April, 1990. This field site was selected because a) the
effluent upon assessment on several different occasions with the effluent alternative
analytical method contained bioconcentratable chemicals in relatively constant
concentrations, b) the flow regime of the site was reasonably simple and had short
flow times, and c) native populations of fish and shellfish were available. Furthermore,
preliminary calculations suggested that concentrations of the chemicals in the effluents
after dilution in the receiving water were high enough to result in measurable tissue
residues in the indigenous organisms.
2.1 Description of Five Mile Creek, Birmingham, Alabama
The site selected for the validation study was a 5.3 km stretch of Five Mile Creek
near Birmingham, Alabama (Figure 2-1). Within the study area, Five Mile Creek
receives discharges from two coking operations, Coke Plant 1 and Coke Plant 2, and
runoff from a railroad maintenance facility and Coke Plant 1 grounds. Five Mile Creek
originates within a residential and commercial area of Birmingham. A United States
Geological Service (USGS) stream flow gauge was located 14.4 km downstream of
the study site at Mineral Springs Republic Road (Figure 2-1).
Indigenous organisms were sampled at four stations in this study. Station 1 was
located immediately upstream of the Coke Plant 1 effluent discharge. This station was
a small pool located behind a low head dam. The Coke Plant 1 discharge pipe
entered Five Mile Creek at the base of the dam downstream from this site. Because
of the dam, this station was deeper (over 3 m deep) and wider than the other three
stations. 140 m upstream of Station 1 on the Coke Plant 1 side of Five Mile Creek, a
drainage ditch for runoff from the parking lots and grounds of Coke Plant 1 entered
the reservoir behind the dam. 40 m upstream of Station 1 on the Coke Plant 1 creek
bank, the water intake for Coke Plant 1 was located. Under typical flow conditions,
water flowed over the dam, across its entire width, at a level of 2.5 to 5.0 cm above
the dam, and this dam was approximately 15 m in width. Large rocks (rip-rap) lined
the edges of the pool, and the bottom was not visible from shore. Organic sediments
were scarce; the bottom substrate consisted primarily of sand or gravel.
Station 2 was located 1.3 km downstream from the Coke Plant 1 effluent
discharge and immediately upstream of the Coke Plant 2 discharge. The creek was
nearly 9 m wide at this point, and depth ranged from relatively shallow on one side to
approximately 1.5 m on the other. At this site, the bottom consisted of bedrock with
large rocks scattered throughout the creek. A fine layer of white sediment lined all
substrates, and any disturbance caused the white, chalky substance to increase
turbidity in the creek. This substance was most likely the result of runoff from a
nearby limestone quarry.
13
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Figure 2-1. Map of sampling stations for aquatic organisms, sediment and caged organisms on Frve Mile Creek.
STATKM 1
COKC
PLAMT1
USCS GAUGMG ETATON
HKJHWAY-79
LOCATED U4 KM
uu»miic*ii man STAHON a
0 Km 1
14
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Station 3 was located at the US 31 bridge, 3.2 km downstream from the Coke
Plant 1 effluent discharge and 1.8 km downstream from the Coke Plant 2 effluent
discharge. The stream was 9 m wide at this point and 0.3 to 1.3 m deep. The bottom
consisted of bedrock, with many large scattered rocks. The sediments were limited to
a gravel substrate with little organic material.
Station 4 was located in an isolated wooded area 5.3 km downstream from the
Coke Plant 1 effluent discharge and 3.9 km downstream from the Coke Plant 2
effluent discharge. The creek bottom consisted of large boulders on a bedrock sheet,
with sandy substrate material and organic sediments. The creek was about 4.6 m
wide and ranged in depth from 0.3 to 1.8 m.
In addition to Stations 1, 2, 3, and 4, six ambient water sampling locations were
used in the study. Ambient (station) 1 was located at the site of the caged Ictalarus
puctatus exposure at Station 1. Ambient 2 was located 40 m upstream of Station 1 at
the water intake for Coke Plant 1 and downstream of the runoff ditch for Coke Plant 1
grounds on the Coke Plant 1 side of the creek. Ambient 3 was located far upstream
of the Station 1 at Springdale Road (see Figure 2-1). Ambient 4 was the runoff ditch
itself from Coke Plant 1. Ambient 5 was located in the reservoir upstream of the dam
and upstream of the runoff ditch for Coke Plant 1 grounds on the Coke Plant 1 side of
the creek. Ambient 6 was the same as Station 3, above.
2.2 Screening of the Effluents
Prior to as well as during the site study, the effluent analytical method was
performed on composites and grab effluent samples. These samples consisted of
a) pre-site study grab samples, and b) samples from the composites taking during the
fourth week of the study. This method detected numerous chemicals: polycyclic
aromatic hydrocarbons (PAHs), alkyl PAHs, and some hetero-PAHs. All of the
effluents contained the same types of chemicals, but the concentrations in the
effluents were quite different between the two dischargers. Data illustrative of all
samples are reported In Appendix A (Tables A-1 through A-5) for grab samples from
Coke Plant 1 (collected on 2/9/90), and Coke Plant 2 (collected on 2/28/90) during the
pre-site study time period. (For Coke Plant 1, data from fraction three are not
presented since interferences from the hydrocarbon "hump" during the GC/MS
analysis prevent successful analysis.)
2.3 Selection of Target Chemicals
For this study, five chemicals, identified with the effluent analytical procedure,
were chosen: biphenyl, phenanthrene, anthracene, fluoranthene, and pyrene. These
chemicals were selected in part since they were typical of all of the chemicals from the
coke plant effluents. Their calculated BCFs ranged from 608 to 3240. and these
chemicals were available in both natural and stable isotope form, i.e., deuterated.
15
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With the stable isotopes, recoveries for each chemical through the analytical
procedure can be determined for each sample.
Selection of the PAHs for this study did cause some concern since PAHs are
metabolized by some aquatic species (7). Metabolism of the chemicals would cause
the predicted residues to be too high in comparison to the measured residues.
However, James (7) has reported that invertebrates metabolize PAHs very slowly, if at
all, and that vertebrates metabolize PAHs easily. Consequently, it was concluded that
if a successful validation was to be performed, the study design must include both
invertebrates and vertebrates. By sampling both phylum of organisms, the importance
of metabolism could possibly be detected as well as addressed.
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METHODS
3.1 Site Study Plan
Measured residue levels in indigenous organisms and caged organisms placed
in situ from Five Mile Creek were compared to residue concentrations predicted for
these organisms.
Residue levels in the organisms were predicted by estimating the in-stream
chemical concentrations and using this data in the residue prediction procedure. In-
stream chemical concentrations were determined by collecting and analyzing four,
seven-day effluent composites taken consecutively over a 28 day period. During this
28 day period, stream and discharge flows were measured. With the flow and
concentration data, the receiving water concentrations were estimated for each
chemical. Subsequently, these concentrations were used in the residue prediction
procedure.
Indigenous and caged organisms were collected at the end of the 28 day period
at sampling stations above and below the discharges. Residue analyses and lipid
content determinations for the resident and caged organisms were performed.
Replicate chemical analyses were performed on the weekly effluent composites
by two analytical laboratories. These analyses included both inter- and intra-
replication for each weekly composite. For the organism samples, duplicate analyses
were performed on selected samples by each laboratory when enough tissue mass
was available. Four replicate samples for each organism collected were assembled in
the field at each sampling station and each laboratory received and analyzed two of
the four replicates.
3.2 Estimation of Residues in Aquatic Organisms
Only a brief description of the residues prediction technique is presented here.
The reader is referred to EPA 1991 (1) for further details.
3.2.1 Prediction of Bioconcentration Factors for Aquatic Organisms
Bioconcentration factors for aquatic organisms are estimated using the multi-
species log BCF-log P correlation developed by Veith and Kosian (2). This correlation
is:
log BCF = 0.79 log P - 0.40 n = 112 r2 = 0.86
This correlation, derived from a data set consisting of 122 BCF values for 13
freshwater and marine species, is typical of all log BCF-log P correlations (3). The
above equation has 95% prediction intervals (note, confidence intervals are much
17
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smaller) of approximately one order of magnitude, and the predicted BCF values are
for organisms with 7.6% lipid content.
The predicted BCF values must be corrected to the appropriate lipid content
before prediction of the tissue residues since numerous fishes and shellfishes have
lipid contents differing from 7.6%. The BCF is directly proportional to lipid content,
and corrections for lipid content are done using a simple proportionality.
3.2.2 Prediction of Bloaccumulatlon Factors for Aquatic Organisms
Bioaccumulation factors are derived by "adjusting" the BCF using a food chain
multiplier (FM) for the organism of concern (1). In equation form,
BAF = FM * BCF
The FM is dependent upon the log P of the chemical and the structure of the
organism's food chain (4-6).
In this site study, the FMs for all of the chemicals under investigation are equal
to 1.0 due to their relatively low log P values and consequently, the BAF and BCF are
equal for this site study. For different chemicals, readers should consult EPA 1991 (1)
to obtain the appropriate FM value.
3.2.3 Prediction of Residues In Aquatic Organisms
The tissue residues for a chemical are calculated by multiplying the BAF, the
product of the BCF and FM terms, after correction for lipid content, by the
concentration of the chemical in the water. In equation form,
[Fish] = BAF ' [Water] = BCF * FM ' [Water]
where [Fish] and [Water] are the concentration of the chemical in the aquatic
organism, and in the receiving water, respectively. Residue concentrations predicted
using the BCF or BAF are for steady state conditions which implies that the
concentration of a chemical in the receiving water is at steady state also.
3.2.4 Metabolism and Prediction of Residues In Aquatic Organisms
The tissue residues predicted using the procedure outlined in Sections 3.2.1
through 3.2.3 assumes that metabolism in vivo does not occur. When metabolism
does occur, the predicted residues will be, in general, larger than those measured in
the organisms since metabolism of a chemical in vivo reduces the concentration of the
chemical in the organism.
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The difference between the actual and predicted tissue residue due to
metabolism is dependent upon the rate of metabolism for each chemical. For
chemicals with slow rates of metabolism, the differences between the predicted and
measured tissue residues will be small, and for chemicals with fast rates of
metabolism, the differences between the predicted and measured tissue residues will
be large.
3.3 Sampling Procedures
3.3.1 Field Sampling Procedures for Effluents
A series of four, seven-day composite effluent samples from both Coke Plant 1
and Coke Plant 2 were collected and sample collection was initiated on March 26 and
27, 1990, respectively. Samples were collected in an iced ISCO sampler equipped
with teflon tubing and a glass collection vessel. The samplers were inspected every
other day, at which time the ice was replenished and the effluent samples were
removed and taken to refrigerated storage. At the end of eacrr seven-day period, the
individual 48-hour samples from each coke plant were composited and immediately
mixed in a Nalgene® carboy. Replicate four liter subsamples were drawn from the
two seven-day composite samples, put on ice, and shipped to the analytical
laboratories. The last seven-day composite samples were collected on April 23rd at
Coke Plant 1 and April 24th at Coke Plant 2.
3.3.2 Field Sampling Procedures for Ambient Grab Samples
Ambient water samples were collected from Five Mile Creek on December 4,
1990. Two 4 liter grab samples were collected at six different locations, put on ice,
and shipped to one of the analytical laboratories using overnight delivery.
3.3.3 Field Procedures for Measuring Stream Flows
Stream flow at two points on Five Mile Creek were measured at various times
during the study by measuring stream velocity and depth at 30 cm (1 foot) intervals
across the creek. Total stream flow was calculated by summing the surface area
velocities across the stream. Stream flows were measured at the US 31 overpass
(Station 3) and approximately 50 meters downstream of Station 1 (near Coke Plant 1).
These sites were selected to provide an approximation of stream flow below the two
discharges included in this study. USGS flow data from Five Mile Creek at Republic
(14.4 km downstream from the US 31 overpass) were acquired for the time period of
the study.
3.3.4 Field Procedures for Sampling Indigenous Organisms
19
-------
Resident organisms were collected on April 25-26 at four stations by
electroshocking the creek for approximately one hour at each site. Shocked
organisms were collected using dip nets and were placed into a cooler containing ice.
Separate coolers were used during field sampling for each station to prevent mixing of
the organism from the different stations. After collection of the organisms, four
replicate samples (when possible) containing a minimum of 30 grams of body mass
were assembled for each type of organism. The only criteria for compositing the
organisms was the minimum amount of mass. Compositing based on sex, size,
reproductive state, age of the organism, etc. was not done. The number of organisms
per sample varied from 1 to 6 organisms. Lepomis sp. (sunfish) and Decapoda
(crayfish) samples were assembled for all stations. Campostoma sp., Hybopsis sp.,
and Notropis hudsonius samples were assembled for some of the stations. All tissue
samples were placed in methanol-rinsed aluminum foil, double wrapped, and labeled.
The samples were immediately frozen in a cooler filled with dry ice, and were held
frozen during transport and storage until analysis.
For Station 1, the Lepomis sp. were collected from the Coke Plant 1 side of the
creek in an area extending from the dam to 50 meters upstream of the dam. The
Decapoda were collected at the base (upstream side) of the dam on the gradual drop
off which leads into the deeper water behind the dam.
For Stations 2, 3, and 4, all organisms were collected in and around the rip-rap
and rubble on both edges of the creek. Approximately 25 meters of shoreline on both
sides were sampled. None of the organisms were collected from open pools or basins
for these stations.
3.3.5 Field Procedures for the Caged Ictalarus puctatus Exposures
Caged Ictalarus puctatus exposures were performed during this study by placing
the caged organisms into Five Mile Creek at Stations 1 and 3. These cages were
constructed out of 20 L Nalgene® carboys as described in Jones and Sloan (8).
At Station 1, four cages were placed on the Coke Plant 1 side of the creek in
1 m of water in a rectangular area ranging from 1 to 2 m from the dam and 1 to 2 m
from the shore. At Station 3, four cages were placed in approximately 1 m of water
at the 1/3 point of the stream from the Coke Plant 1 side of the creek.
Ictalarus puctatus were obtained from a commercial catfish supplier, Pettrt
Farms, Olountsville, Alabama and were 4-6 grams in size. On April 5th, eight cages
containing 20 to 30 fish each were placed into Five Mile Creek at Stations 1 and 3. At
this time, a group of Ictalarus puctatus was retained for background analysis. These
cages were monitored and fed daily with commercial catfish food obtained from Pettrt
Farms. A fair amount of daily mortality was noted, and on each day, all dead fish
were removed. On April 15th, all surviving fish died in all cages.
20
-------
New fish were obtained from Pettit Farms and the eight cages were put back
into Five Mile Creek on April 18th at Stations 1 and 3. These cages were monitored
and fed daily. On April 28th, the Station 1 cages were removed from the stream.
Because of increased stream turbidity and flow due to a overnight storm, the Station 3
cages were impossible to locate on the 28th of April. On May 2nd, these cages were
found and removed from the stream.
For the background and the Station 1 Ictalarus puctatus (taken from the cages
on April 28th), two and four replicate samples containing a minimum of 30 grams of
body mass were prepared, respectively. These tissue samples were placed in
methanol-rinsed aluminum foil, double wrapped, and labeled. The samples were
immediately frozen in a cooler filled with dry ice, and were held frozen during transport
and storage until analysis.
For the Station 3 Ictalarus puctatus (taken from the cages on May 2nd), all of the
organisms were wrapped together in methanol-rinsed aluminum foil and were frozen
and shipped to Battelle-Great Lakes on dry-ice. Upon arrival, this sample was partially
thawed and then subdivided into four samples consisting of a minimum of 30 grams
per sample. These subsamples were placed in methanol-rinsed aluminum foil, double
wrapped, and labeled. The samples were frozen and were held frozen during
transport and storage until analysis.
3.4 Analytical Procedures
3.4.1 Effluent Analysis Procedure
Only a brief account of the procedure for detecting and identifying
bioconcentratable chemicals in effluents will be present here. Readers are referred to
Appendix B of EPA's guidance (1) for further details.
A 10 L effluent sample is spiked with three surrogate compounds, d10-biphenyl,
13Ce-1,2,4,5-tetrachlorobenzene, and 13Ce-hexachlorobenzene, and extracted with
hexane. The hexane extract is subsequently cleaned up using surfuric acid, and
concentrated to a volume of 0.50 ml_. The extract is chromatographed using reverse
phase HPLC, and three fractions are collected. The fractions are extracted,
concentrated to 0.10 mL, and spiked with the internal standard, dia-chrysene. The
fraction extracts are analyzed using capillary gas chromatography with full scan
electron impact ionization mass spectrometry (GC/MS).
Each chromatographic peak in the GC/MS chromatograms is quantified using the
response factor calculated from its appropriate surrogate. For fractions one, two, and
three, the quantification surrogates are d10-biphenyl, C6-1,2,4,5-tetrachlorobenzene,
and 13Ce-hexachlorobenzene, respectively.
21
-------
For each fraction, all chromatographic peaks are reverse-searched against
(compared with) the Chemicals of Highest Concern (CMC) mass spectral library.
Those chemicals not identified with the CMC search with effluent concentrations above
100 ng/L, are then reversed-searched against the EPA/NIH/NBS mass spectral library.
Peaks with fits of greater than 70% are considered tentatively identified. For each
tentatively identified component, a list of the best mass spectral library identifications
(up to a total of ten identifications) is reported along with the percent fit values.
3.4.2 Weekly Effluent Composite Analysis
The weekly effluent composite samples were analyzed at two different
laboratories in this study, Battelle-Columbus and Environmental Research Laboratory-
Duluth (ERL-D). The analytical methods used at both laboratories were very similar,
and the concentrations reported for the five target chemicals were nearly identical for
the two laboratories. Comparable data between the two laboratories was obtained by
the use of an internal standard quantification method, deuterated surrogates for
determining compound recoveries, and reporting of the data after recovery correction.
The chemicals used for recovery correction were the deuterated form of the target
chemical, e.g., for biphenyl, recovery corrections were based upon d10-biphenyl.
The analytical procedures for both labs consisted of spiking a known volume of
effluent, i.e., 1 L or 900 ml, with d10-biphenyl, d10-phenanthrene, d,0-anthracene, d10-
fluoranthene (Battelle only), and d10-pyrene at concentrations similar to the target
chemical concentrations. In general, for the effluents from Coke Plants 1 and 2, spike
concentrations of 0.1 and 1.0 ug/L were used, respectively. The spiked effluents were
extracted three times using hexane, 60 ml_ per extraction. The hexane was dried
using sodium sulfate, concentrated using a Kudema-Danish concentrator (KD) to ca.
10 ml_ and reduced to 1.0 or 0.10 using a gentle stream of nitrogen. These extracts
were spiked with the internal standard, d12-chrysene, at a 10 mg/L concentration.
GC/MS analysis using selected ion monitoring (SIM) was performed, and
quantifications were performed using an internal standard method with a 4 or 5 point
calibration curve using the M* ion for each chemical. Quantification standards
contained the internal standard d12-chrysene and both the deuterated and native forms
of the five target chemicals except for ERL-D's standards which did not contain d10-
fluoranthene. For biphenyl, phenanthrene, anthracene, fluoranthene and pyrene,
recovery corrections were made using the recoveries of d10-biphenyl, d,0-
phenanthrene, d10-anthracene, d10-fluoranthene (Battelle) or d10-pyrene (ERL-D), and
d10-pyrene, respectively.
3.4.3 Ambient Water Samples Analysis
Ambient water samples were analyzed at ERL-D with the same procedure used
for the weekly effluent composite samples, see Section 3.3.
22
-------
3.4.4 Tissue Analysis
Tissue samples were analyzed at two different laboratories in this study, Battelle-
Columbus and ERL-D. As with the procedures used for the analysis of weekly effluent
composites, the analytical methods used at both laboratories were very similar and the
concentrations of the five target chemicals were not significantly different between the
two laboratories. Comparable data was obtained due to the use of appropriate
analytical techniques as described in Section 3.4.2.
Battelle: The thawed whole organisms were finely chopped with a Hobart
mincer, and a 20-gram aliquot of tissue was transferred to a centrifuge bottle
containing magnesium sulfate and methylene chloride. The tissue was spiked with the
deuterated compounds used for the effluent analyses and extracted with a Polytron*
tissue homogenizer for two minutes. The extract was then transferred to an alumina
column, and the homogenate was extracted twice more by shaking with additional
methylene chloride. Each extract was passed through the alumina column, and the
methylene chloride eluate was concentrated by using a Kudema~Danish (K-D)
concentrator to a volume of 2.0 ml_.
One mL of the concentrated extract was injected onto a gel permeation
chromatograph (GPC) to remove lipids. The collected fraction was concentrated to
about 10 mL by K-D, exchanged to hexane, and reduced to 100 u.L by natural
evaporation. The prepared samples were analyzed using the GC/MS procedures
used for the effluent analysis.
Lipid contents were determined by extracting known amount of tissue (1 to 2
grams) with methylene chloride, evaporating the solvent, placing the extract into an
oven at 130°C for 60 minutes, and after cooling, weighing the extract. Lipid content
was calculated by dividing the extracted mass by the mass of the extract tissue.
ERL-Duluth: The frozen whole organisms, consisting of one to six animals per
sample, were finely chopped and mixed together using a Waring blender. A 20-gram
aliquot of tissue was mixed with sodium sulfate, spiked with the deuterated
compounds used for the effluent analyses, and extracted using methylene
chloride:hexane (1:1) with a Soxhlet extractor. The extract was concentrated to 10 mL
using a K-D concentrator and then, to dryness with a gentle stream of nitrogen gas.
The K-D lower tubes with extract were weighed and then diluted with methylene
chloride for GPC to remove the lipids. After GPC, the extract was concentrated and
subjected to silica gel chromatography to remove cholesterol-like compounds. The
extract was concentrated to a volume less than 1 mL and spiked with d12-chrysene.
The prepared samples were analyzed using the same GC/MS procedures used for the
effluent analysis.
23
-------
The lipid content for each analysis was determined by dividing the lipid mass
measured during the analysis by the tissue sample mass placed into the Soxhlet
extractor.
24
-------
RESULTS AND DISCUSSION
4.1 Expected Tissue Residues Trends
Since the technique used for predicting residues in aquatic organisms does not
account for metabolism, the following general statements about the comparison of the
measured and predicted tissue residues for the five PAHs can be made prior to
examination of any of the data.
a) Evidence on the metabolic abilities of aquatic invertebrates and vertebrates
for PAHs suggests that the agreement between the measured and
observed tissue residues should be better for the Decapods than for the
Lepomis sp. organisms collected in the site study. James (7) has reported
for aquatic species that invertebrates do not (or very slowly) metabolize
PAHs, and that vertebrates metabolize PAHs fairly easily.
b) The residues predicted for the Lepomis sp. should be larger than the
measured residues due to metabolism. For the Deoapoda, this bias should
not exist due to the limited metabolic ability of the organisms.
c) Evidence on the relative rates of metabolism for the five chemicals under
investigation suggest that with increasing size, poorer agreement between
the measured and predicted tissue residues should exist for the Lepomis
sp. than for with Decapoda (10). The rate of metabolism for PAHs in
fishes appears to increase with increasing size of the molecule (10).
These general statements assume that the residue prediction technique is valid
and provides reliable predictions, and that the metabolic behavior of PAHs in both of
the evaluated species occurs as stated above.
4.2 Flow Data for Five Mile Creek and Effluents
The stream flows were measured on Five Mile Creek at Station 3 and 50 meters
downstream of Station 1 five or six times during the study and these values are
reported in Table A-6. (Note, Tables A-# are in Appendix A of this report.) Daily flows
for the USGS gauge at Mineral Springs-Republic Road, 14.4 km downstream from
Station 3, were measured, and these flows are reported in Table A-6 for the length of
the study, March 26 through April 26, 1990. With the measured flows, two regression
models were constructed (y=mx+b) to estimate the stream flows at Stations 1 and 3
from the USGS daily flows at Republic (Table A-7).
The use of these equations for estimating stream flows at Stations 1 and 3
assumes that flows at Stations 1 and 3 were directly proportional to the measured
flows at the Republic gauge. However, this proportionality was not always true. Other
streams enter Five Mile Creek between Station 3 and the USGS gauge at Republic,
and due to differences in watershed areas and hydraulics as well as differences in
25
-------
rainfall during storm events, the assumption of direct proportionality was not always
valid. The flows estimated using the derived equations are our best estimate for the
average daily flows for Stations 1 and 3. To obtain some estimate of the error
associated with the predicted flows, confidence and prediction intervals for the
estimated flows were determined using the regression statistics for both equations. In
Table A-7, the estimated flow and the 95% confidence and prediction intervals for
measured flows of 1.00, 1.25, 1.50, 1.75, 2.00, and 2.25 m3/s for both Stations 1 and
3 are reported. The 95% prediction intervals for the estimated flows ranged from ±24
to ±42% and ±25 to ±39% of the estimated flows at Stations 1 and 3, respectively.
In Figure 4-1, the estimated and measured daily flows have been plotted for the
time period of the study. During the time period of the site study, daily flows
decreased by approximately a factor of 2 and short term increases in flow due to
storm events were observed five or six times. The average daily stream flows for
Stations 1 and 3, computed from the estimated flows, were 0.748 and 0.981 m3/s with
coefficients of variation of 13.7% and 10.9%, respectively (Tables 4-1 and A-6).
The daily discharge flows for both dischargers are reported in Table A-8 and are
plotted in Figures 4-2 and 4-3 for the time period of the study. These flows were
measured and reported to the State of Alabama by each discharger as part of their
NPDES permit. In Table 4-1, the flow data are summarized for each discharger.
For Coke Plant 2, the average discharge flow and its coefficient of variation were
0.184 m3/s and 8.16%, respectively. For Coke Plant 1, the average discharge flow
and its coefficient of variation were 0.00478 m3/s and 42.1%, respectively. The larger
coefficient of variation for Coke Plant 1 was caused by the stoppage of discharge flow
on 2 days during the site study.
4.3 In-Stream Effluent Concentrations
In 1983, Mount et al. (9) performed dye studies on Five Mile Creek to determine
mixing characteristics for the discharges from both coking operations. For Coke Plant
1, dye studies were performed in February and October of 1983 and complete mixing
of the effluent with Five Mile Creek occurred 762 m and 15 m downstream of the
discharge, respectively. These dye studies were performed with stream flows of 1.95
and 0.292 m3/sec at 500 m below the discharge and with discharge flows of 0.008 and
0.009 m3/sec, respectively. For Coke Plant 2, one dye study was performed in
October, 1983, and complete mixing of the effluent with Five Mile Creek occurred 457
m downstream of the discharge with stream and discharge flows of 0.527 and 0.120
m3/sec.
Stations 2 and 3 for this study were 1300 and 1800 m downstream of the
discharge points for Coke Plants 1 and 2, respectively. These distances well exceed
those, i.e., 752 and 457 m, determined with the dye studies for complete mixing in the
26
-------
Figure 4-1. Measured and Predicted Flows on Five Mile Creek.
2.5
o
0.5
I ,
A"
. i
26 28 30 1
5 7 9 11 13 15 17 19 21
March/April 1990
Republic Station 1 Stations
23 25
27
-------
Figure 4-2. Measured Discharge Flow for Coke Plant 1.
0.01
0.008
CO 0.006
E,
§ 0.004
LL
0.002
i . i
26 28 30 1 3 5 7 9 11 13 15 17 19 21 23 25
March/April 1990
28
-------
Rgure 4-3. Measured Discharge Flow for Coke Plant 2.
0.24
0.22
CO
CO 0.2
*
*
0.18
0.16
0.14
i . i . i . i
26 28 30 1
5 7 9 11 13 15 17 19 21 23 25
March/April 1990
29
-------
Table 4-1. Average Predicted Stream Flows, Average Discharge Flows, and
Average In-Stream Effluent Concentration for Two Coking Plants on Five
Mile Creek for March 26, 1990 - April 26, 1990.
Average Flows
Average
(mVs)
Standard
Deviation
Coefficient
of Variation
Range
(m3/s)
Station 1*
Station 3
Coke Plant 1 Effluent
Coke Plant 2 Effluent
0.748
0.981
0.00478
0.184
0.102
0.107
0.00201
0.0150
32
32
32
32
13.7 0.613-0.979
10.9 0.840-1.223
42.1 0.00-0.00814
8.16 0.149-0.223
In-Stream Effluent Concentration
Average
Standard Coefficient
Deviation n of Variation Range
Station 1 (Station 2)
Station 3
0.644
19.0
0.282
2.80
32
32
43.9
15.8
0.00-1.153
13.9-24.0
' Approximately 50 m downstream of Station 1.
30
-------
creek. These results suggest that the effluents from Coke Plants 1 and 2 are
completely mixed with Five Mile Creek at Stations 2 and 3 for this study, respectively.
Daily in-stream effluent concentrations for the discharges from Coke Plants 1
and 2, assuming complete mixing, were calculated using the predicted stream and
measured discharge flows for Stations 2 and 3 (Table A-9, Figures 4-4 and 4-5). For
Station 2, flows predicted for 50 m downstream of Station 1 and measured discharge
flows for Coke Plant 1 were used. For Station 3, flows predicted for Station 3 and
measured discharge flows for Coke Plant 2 were used. For both discharges, a
gradual increase in the in-stream effluent concentration occurred during the study
period as illustrated in Figures 4-4 and 4-5. For the effluents from Coke Plants 1 and
2, the coefficients of variation for the in-stream effluent concentrations were 43.9%
and 15.8% at Stations 2 and 3, respectively (Tables 4-1 and A-9).
4.4 Analysis of the Weekly Effluent Composites for the Five Target Chemicals
Replicate analyses were performed on each weekly effluent composite for the
five target chemicals. The individual determinations are reported in Tables A-10
through A-19. In Table 4-2, the average weekly and grand mean concentrations for
the five target chemicals are reported for both effluents for the period of the study.
For each chemical, effluent concentrations were relatively constant over the 4 week
study period for both effluents (Table 4-2).
4.5 In-Stream Chemical Concentrations for the Five Target Chemicals
The daily in-stream concentrations for each chemical in Five Mile Creek were
computed for Stations 2 and 3 using the estimated daily stream flows, the daily
discharge flows, and the chemical concentrations in the weekly effluent composite
samples (Tables A-20 through A-24). The concentrations for biphenyl, phenanthrene,
anthracene, fluoranthene, and pyrene are plotted in Figures 4-6, 4-7, 4-8, 4-9, and 4-
10 and are summarized in Table 4-3.
For all five chemicals, their coefficients of variation for the in-stream chemical
concentrations at both Stations 2 and 3 were approximately 40%, except for biphenyl
at Station 2. These coefficients of variation include variability due to changes in
discharge and stream flows, to changes in chemical concentrations in the discharge,
and to analytical measurement. Most of the variability in the in-stream chemical
concentrations was due to the large variability in discharge flow from Coke Plant 1.
The only chemical with substantially larger variability than that observed for the
discharge flow for Coke Plant 1 was biphenyl, and its variability was due largely to
analytical measurement since it was not detected in two of the effluent composite
samples.
4.6 Indigenous Organisms
31
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4.6.1 Tissue Data
Two species of resident organisms, Lepomis sp. and Decapods, were analyzed
for the five target chemicals by two laboratories. These results are reported in Tables
A-25, A-26, A-27, and A-28 and are summarized in Table 4-4.
For the Lepomis sp., inter- and intra-laboratory agreement was good for
phenanthracene, anthracene, fluoranthene, and pyrene. However, for biphenyl, there
was a substantial difference in reported residue concentrations between the
laboratories for all four sampling Stations. Examination of the procedural blanks
performed with these analyses revealed that one laboratory had very high background
concentrations which suggests that the difference in reported residue concentrations
was due to in-house background contamination. Consequently, all of the biphenyl
tissue data for both resident organisms from this laboratory were not used in the
analysis of the data for the site study.
For the Decapods, inter- and intra-laboratory agreement was good for
phenanthracene, anthracene, fluoranthene, and pyrene. However, one
Decapodasample, which was analyzed in duplicate, for Station 3 had residue
concentrations for all five target chemicals which were much higher than the other
replicates samples analyzed for that Station, e.g., for phenanthrene, residue
concentrations of 39.4, 48.1, 64.6, and 2720 ng/kg were determined. Because this
one sample was so different from the three replicate samples, we believe that this
sample was an outlier and was not representative of resident organisms for this
Station. Consequently, in the summary data in Table 4-4 and in the analysis of the
data for the site study, this sample was not included.
4.6.2 Residue Trends
The average tissue residue concentrations for four of the target chemicals,
biphenyl, anthracene, fluoranthene, and pyrene, followed the same general trend from
Station 1 to Station 4 for the Lepomis sp. and Decapods (Table 4-4). The average
residue levels in the organisms from Station 1 (the reservoir upstream of the Coke
Plant 1 discharge) were relatively low, and at Station 2, which is 1300 m downstream
of the discharge point for Coke Plant 1, the average tissue residues were much higher
than at Station 1. At Station 3, which is 1800 m downstream of the discharge from
Coke Plant 2, the average residues were higher than Station 1 but slightly less than
Station 2. At Station 4, the average residue concentrations were similar to those
determined for the organisms from Station 3.
For the fifth target chemical, phenanthrene, the average residue concentrations
for Station 1 were much higher than those determined for the other 4 target chemicals.
32
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Table 4-2. Concentration of Target Chemicals in Weekly Effluent Composite Samples from Two
Coking Plants on Five Mile Creek, Birmingham, Alabama.
Concentration in Effluent. ODD*
Weekl Week 2 Week 3 Week 4
Std. Coefficient
Avg. Dev. of Variation, %
Coke Plant 1 Effluent"
Biphenyl 0 0 1.88 0.34 0.56 (0.91) 162
Phenanthrene 16.6 12.7 15.6 15.5 15.1 (1.68) 11.1
Anthracene 7.26 4.45 6.68 7.22 6.40 (1.33) 20.8
Fluoranthene 21.1 17.4 21.4 21.4 20.3 (1.95) 9.62
Pyrene 14.4 12.0 15.7 17.4 14.9 (2.28) 15.3
Coke Plant 2 Effluent0
Biphenyl
Phenanthrene
Anthracene
Fluoranthene
Pyrene
0
0.02
0.02
0.19
0.10
0.04
0.03
0.02
0.23
0.12
0.04
0.07
0.02
0.23
0.12
0.06
0.20
0.02
0.28
0.13
0.04 (0.03)
0.08 (0.08)
0.02 (0.00)
0.23 (0.04)
0.12 (0.01)
71.9
104
0.0
15.8
10.7
Recovery and blank corrected.
The reported values for the weekly composites are the average of the replicate analyses.
Number of replicate analyses performed for weeks 1,2,3, and 4 were 2, 2, 4, and 4,
respectively.
The reported values for the weekly composites are the average of the replicate analyses.
Number of replicate analyses performed for weeks 1, 2, 3, and 4 were 4, 4, 6, and 4,
respectively.
33
-------
Figure 4-4.
In-Stream Effluent Concentration for Coke Plant 1.
1.4
1.2
1
0.8
ft*
0.6
0.4
0.2
-------
Figure 4-5.
In-Stream Effluent Concentration for Coke Plant 2
26
24
22
20
s
16
14 -
12
.i.i
1 _. j .1 . i
26 28 30 1
7 9 11 13 15 17 19 21 23 25
March/April1990
35
-------
Figure 4-6.
In-Stream Concentration for Biphenyl.
25
20
D)
.0
"H
I
o
O
15
10
. r . i . i . i
26 28 30 1
5 7 9 11 13 15
March/April 1990
Station2 Stations
17 19 21 23 25
36
-------
Figure 4-7.
In-Stream Concentration for Phenanthrene.
200
O)
150
.g
2? ioo
-------
Figure 4-8.
In-Stream Concentration for Anthracene.
100
80
CD
§ 60
CO
"c
o
O
40
20
26 28 30 1
5 7 9 11 13 15
March/April 1990
Station 2 Station 3
17 19 21 23 25
38
-------
Figure 4-9.
In-Stream Concentration for Fluoranthene.
300
250
.o
j3 150
8
o
o
100
50
i . i . i . i . i . i . i
i
26 28 30 1
5 7 9 11 13 15 17
March/April 1990
Station 2 Station 3
19 21 23 25
39
-------
Figure 4-10.
In-Stream Concentration for Pyrene.
200
O
150
g
& 100
§
o
O
50
J ,1 , I , I , I . I . I . I .
I . I
26 28 30 1
57 9 11 13 15 17 19 21 23 25
March/ApriM990
Station 2 Station 3
40
-------
Table 4-3. Grand Mean Daily In-Stream Concentration of the Five Target Chemicals
for Stations 2 and 3 for Five Mile Creek.
Average
(ng/D
Standard
Deviation
Coefficient
of Variation
Station 2
Biphenyl
Phenanthrene
Anthracene
Fluoranthene
Pyrene
4.06
97.3
41.6
132.
97.6
6.39
42.8
19.3
59.0
45.8
157.
44.0
46.4
44.8
47.0
Station 3
Biphenyl
Phenanthrene
Anthracene
Fluoranthene
Pyrene
Anthracene
10.8
93.8
35.3
146.
96.8
35.3
7.22
35.4
14.5
46.1
35.4
14.5
67.0
37.7
41.0
31.6
36.5
41.0
41
-------
Table 4-4. Average Tissue Residues (ppb) in Two Species of Resident Organisms
for Five Mile Creek.
Station 1 Station 2 Station 3 Station 4
Decapoda (Crayfish)
Biphenyl 1.16(2)' 16.7# (2) 3.13" (1) 2.97(2)
Phenanthrene 54.9 (4) 171.* (5) 50.7" (3) 41.9 (4)
Anthracene 3.40(4) 55.3* (5) 21.4" (3) 15.6 (4)
Fluoranthene 23.4 (4) 228.* (5) 68.6" (3) 65.9 (4)
Pyrene 16.6 (4) 207/ (5) 50.3" (3) 53.2 (4)
Lepomis sp. (Sunfish)
Biphenyl
Phenanthrene
Anthracene
Fluoranthene
Pyrene
6.34 (2)
77.0 (4)
8.33 (4)
19.2 (4)
9.86 (4)
7.15 (3)
65.1 (5)
18.2* (5)
27.2 (5)
10.2 (5)
9.60 (2)
57.9 (4)
18.1* (4)
32.8 (4)
13.3 (4)
5.37 (3)
27.7 (5)
13.4 (5)
22.3 (5)
13.2 (5)
8 Number of different organism samples analyzed for that station are in
parentheses.
b Outlier not used in calculating average residue concentration, see Section 4.5.1.
Significantly greater than residue levels for Station 1, Dunnett's test, 95%
confidence level.
# Significantly greater that residue levels for Station 1, one way analysis of
variance, 95% confidence level.
42
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In addition, for nearly all sampling stations, average tissue residues for phenanthrene
for Station 1 were much higher than those determined for Stations 2, 3, and 4 for both
species of organisms. We cannot explain this anomaly of having higher upstream,
Station 1, residue concentrations for phenanthrene. However, this trend suggests that
in-stream concentrations for phenanthrene might have been higher at Station 1 than at
Stations 2, 3, and 4. Higher concentrations for phenanthrene could possibly occur if a
point and/or nonpoint source containing phenanthrene was discharged into Five Mile
Creek upstream of Station 1 during the site study. Ambient water samples, taken after
the site study, suggest that a point source of phenanthrene might exist upstream of
station 1, see Section 4.7.
The increase in residue concentrations at Stations 2 and 3 for both species of
organisms demonstrates that accumulation of the chemicals discharged does occur in
the receiving water organisms for Five Mile Creek. In the 30 residues determined for
Stations 2, 3, and 4, 80% of the measured residues were greater than their
corresponding residues at Station 1. Excluding phenanthrene, 95.8% (23 out of 24) of
the measured residues were greater than their corresponding residues at Station 1.
4.6.3 Significance Testing of Residues
To further evaluate the increases in tissue concentrations, an analysis of
variance and then Dunnett's test was performed for each chemical to determine if the
residues in the Lepomis sp. and Decapoda from the downstream stations (Stations 2,
3, and 4) were significantly greater than residues measured in the upstream
organisms at Station 1 (Table 4-4). (Note, for the Decapoda biphenyl data, Dunnett's
test could not be performed due to the limited number of samples, and thus, a simple
t-test using the pooled standard deviation was used).
For the Decapoda, the tissue concentrations for biphenyl, phenanthrene,
anthracene, fluoranthene, and pyrene, at Stations 2 were significantly greater, 95%
confidence level, than those determined for the upstream site, Station 1. For stations
3 and 4, none of the residue levels for the Decapoda were significantly greater, 95%
confidence level, than those determined for Station 1. For the Lepomis sp., one
chemical, anthracene, at Stations 2 and 3 was significantly greater than the residues
measured for Station 1. For the other four target chemicals, none of the Lepomis sp.
tissue residues for Stations 2, 3, and 4 were significantly greater than the tissue
residues measured for Station 1.
The number of residues which were significantly greater than Station 1 was
much smaller than the number of residues which were just greater than the residues
for Station 1, i.e., 23% (7 of 30) vs. 80% (24 of 30), respectively. The lack of
statistically significant increases in tissue residues for the stations downstream of
Station 1 may have been due to the limited number of tissue samples analyzed and/or
to the organism compositing technique which did not consider the size of the
individuals in the samples. If larger numbers of sample analyses had been performed
and/or larger as well as consistent numbers of organisms used for each species, the
variances of the measured tissue residues at each Station might have been smaller.
43
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Smaller variances would allow smaller differences between Station 1 and the other
sampling Stations to be statistically significant. Even so, the combination of having
80% of the residues downstream of Station 1 higher than the residues for Station 1
and that 23% of these residues were statistically significant, strongly suggests that the
accumulation of these chemicals from the effluents is occurring with the resident
organisms in Five Mile Creek.
4.6.4 Comparison of Predicted and Observed Residues
To evaluate the residue prediction procedure, residues were predicted and then
compared to the measured residues for the indigenous organisms. By using the
residue prediction procedures, values for the log P, BCF, FM, and BAF were derived
for each chemical (Table 4-5). Subsequently, residues for the Lepomis sp. and
Decapoda organisms were predicted for Stations 2 and 3 on Five Mile Creek by using
the derived BAFs (Table 4-5), the average in-stream chemical concentrations (Table
4-3), and the average lipid content for each species (Table A-28). To these values,
the average residue levels for the upstream site, Station 1, were added and these
predictions are reported in Tables 4-6 and 4-7.
For the Decapoda organisms, the predicted tissue residues were, in general,
within a factor of 3 or less of the measured tissue residues for Stations 2 and 3, and
the predicted residues tended to be slightly lower and higher at Stations 2 and 3,
respectively (Table 4-6). The ratio of the observed to predicted tissue residues ranged
from 0.39 to 8.57 (Table 4-6).
For the Lepomis sp., the predicted tissue concentrations were, in general,
higher than the observed values for both Stations 2 and 3. The ratio of the observed
to predicted ranged from 0.05 to 0.96 for both Stations 2 and 3. With increasing BCF
value for the five target chemicals, wider disagreement between the measured and
predicted values was observed (Table 4-7).
The better agreement between the predicted and observed tissue residues for
the Decapoda, in comparison to the Lepomis sp., was expected for this study (Section
4.1). The predicted BCF values for the five target chemicals assumes that metabolism
does not chemical occur. However, polycyclic aromatic hydrocarbons are metabolized
by vertebrates such as fish and are slowly (if at all) metabolized by invertebrates such
as arthropods (7). Furthermore, studies have shown that for unsubstrtuted polycyclic
aromatic hydrocarbons, their rate of metabolism is dependent upon the size, e.g.,
number of aromatic rings or molecular weight. In general, larger unsubstrtuted
polycyclic aromatic hydrocarbons tend to have faster rates of metabolism than smaller
polycyclic aromatic hydrocarbons (10).
When a chemical is metabolize in an aquatic organism, the true BAF value for
the chemical will be lower than the BAF predicted using the residue prediction
procedure (which assumes no metabolism). Consequently, the predicted tissue
concentrations will be larger than those measured in the indigenous organisms. In
this study, the ratios of the observed to predicted tissue residues were lower for the
44
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Table 4-5. Residue Prediction Parameters.
Compound Log P BCF FM BAF
Biphenyl
Pnenanthrene
Anthracene
Fluoranthene
Pyrene
4.03
4.49
4.49
4.95
4.95
608
1400
1400
3240
3240
1
1
1
1
1
608
1400
1400
3240
3240
45
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Table 4-6. Predicted and Measured Decapoda Tissue Concentrations for Five
Mile Creek.
Station 2
QSAR
BCF
608
1400
1400
3240
3240
Target
Compound
Biphenyl
Phenanthrene
Anthracene
Fluoranthene
Pyrene
Predicted
Cone, in
Tissue
(ppb)
1.95
89.6
22.0
160.
118.
Observed
Cone, in
Tissue
(ppb)
16.7
171.
55.3
228.
207.
Ratio of
Observed to
Predicted
Cone, in
Tissue
8.57
1.74
2.51
1.42
1.76
Station 3
608
1400
1400
3240
3240
Biphenyl
Phenanthrene
Anthracene
Fluoranthene
Pyrene
3.26
97.0
19.2
175.
117.
3.13
50.7
21.4
68.6
50.3
0.96
0.52
1.11
0.39
0.43
Predicted Tissue Concentration = BCF x FM x Decapoda lipid/QSAR lipid x (In-stream
Chemical Concentration) + Upstream (Station 1) Tissue Concentration. All values
corrected for recoveries. Decapoda lipid content 2.43%, QSAR lipid 7.6%.
46
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Table 4-7. Predicted and Measured Lepomis sp. Tissue Concentrations for Five
Mile Creek.
Station 2
QSAR
BCF
608
1400
1400
3240
3240
Target
Compound
Biphenyl
Phenanthrene
Anthracene
Fluoranthene
Pyrene
Predicted
Cone, in
Tissue
(ppb)
7.71
153.
40.8
257.
186.
Observed
Cone, in
Tissue
(ppb)
7.15
65.1
18.2
27.2
10.2
Ratio of
Observed to
Predicted
Cone, in
Tissue
0.93
0.43
0.45
0.11
0.05
Station 3 608
1400
1400
3240
3240
Biphenyl
Phenanthrene
Anthracene
Fluoranthene
Pyrene
9.99
150.
35.9
282.
184.
9.60
32.8
18.1
32.8
13.3
0.96
0.39
0.50
0.12
0.07
Predicted Tissue Concentration = BCF x FM x Lepomis sp. lipid/QSAR lipid x (In-
stream Chemical Concentration) + Upstream (Station 1) Tissue Concentration. All
values corrected for recoveries. Lepomis sp. lipid content 4.23%, QSAR lipid 7.6%.
47
-------
Lepomis sp. than for the Decapoda. These results are consistent with the expected
metabolic abilities of the species, i.e., low or almost nonexistent metabolic activity for
the Decapoda and higher (or substantial) metabolic activity for the Lepomis sp.
(Tables 4-6 and 4-7).
Considering the rates of metabolism for the five target chemicals, one would
expect that the rates of metabolism should follow the general order of biphenyl <
phenanthrene = anthracene < fluoranthene = pyrene for the five chemicals in this
study. (Note, the number of aromatic rings in the five target chemicals are 2, 3, 3, 4,
and 4, respectively.) In this study, the Lepomis sp. residue data mimics this expected
metabolic behavior, i.e., with increasing size of the chemical, the differences between
the measured and observed tissue residues increases. In addition, for the
Decapodawhere metabolism is expected to be low or almost nonexistent, this
metabolic behavior is not observed.
Overall, the agreement between the measured and predicted tissue residues
were quite reasonable for both species of organisms considering that metabolism of
the chemicals was ignored in the residue prediction procedure. In this study, the
ratios of the observed to predicted tissue residues were lower for the Lepomis sp. than
for the Decapoda which suggests that metabolism was occurring with the Lepomis sp.
The differences between the measured and predicted tissue residues were consistent
with the expected differences in the species metabolic activity and in the rates of
metabolism for the parent chemicals.
4.6.5 Comparison of Measured and Predicted Residues: Error Analysis
To further evaluate the predicted tissue residues, a propagation of error
analysis was performed for the predicted tissue concentrations for each chemical for
Stations 2 and 3 so that confidence limits could be derived for the predicted values.
The derived 95% and 99% confidence limits for each chemical residue are reported in
Tables 4-8 and 4-9 for the Decapoda and Lepomis sp., respectively.
The error analysis was performed by using the mean and standard deviations
for the in-stream and Station 1 tissue concentrations from Tables 4-3 and 4-4 for each
chemical. The predicted logarithm of the BCF and its standard deviation (for the
predicted value) were obtained from the regression equation and data of Ve'rth and
Kosian (2). The mean lipid content and its standard deviation for each species were
taken from Table A-28. With these values, a Monte Carlo analysis was performed to
derive the error estimates for the predicted the tissue concentrations. Note, the
predicted tissue concentrations have a lognormal like distribution which is caused by
the antilog transformation for the BCF parameter.
The 95% confidence limits for both species for all chemicals are approximately
one order of magnitude. This large uncertainty is caused mostly by the variability
associated with the predicted BCF. We estimate that more than 95% of the total
uncertainty in the predicted tissue residues is due to the predicted BCF.
48
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Table 4-8. Confidence Limits for the Predicted Decapoda Tissue Concentrations for
Five Mile Creek.
Tissue Concentration
Predicted
(ppb)
Station 2
Biphenyl
Phenanthrene
Anthracene
Fluoranthene
Pyrene
Station 3
Biphenyl
Phenanthrene
Anthracene
Fluoranthene
Pyrene
1
.95
89.6
22
160
118
3
97
.0
t
.26
.0
19.2
175
117
,
,
Observed
(ppb)
16.
171.
55.
228.
207.
3.
50.
21.
68.
50.
7
95%
Confidence
Limits
0
.00
49.4
3
3
26
.28
.3
17.8
13
7
4
6
3
0
50
3
32
21
.51
.6
.54
.2
.7
13.1
492.
194.
1394.
1047.
24.2
465.
161.
1471.
992.
99%
Confidence
Limits
0.
41.
0.
5.
0.
0.
44.
1.
22.
14.
00
4
00
96
00
00
0
85
7
7
30
1038
418
3066
2277
.9
.
53.2
966
353
3101
2167
49
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Table 4-9. Confidence Limits for the Predicted Lepomis sp. Tissue Concentrations
for Five Mile Creek.
Tissue Concentration
Station 2
Biphenyl
Phenanthrene
Anthracene
Fluoranthene
Pyrene
Station 3
Biphenyl
Phenanthrene
Anthracene
Fluoranthene
Pyrene
Predicted
(ppb)
7.71
153.
40.8
257.
186.
9.99
150.
35.9
282.
184.
Observed
(ppb)
7.
65.
18.
27.
10.
9.
57.
18.
32.
13.
15
1
2
2
2
60
9
1
8
3
95%
Confidence
Limits
0
.00
57.5
6
28
.17
.5
16.4
3
59
6
41
24
.17
.2
.46
.3
.3
27.1
820.
332.
2342.
1778.
45.9
772.
272.
2468.
1669.
99%
Confidence
Limits
0.
38.
0.
0.
0.
0.
42.
2.
27.
14.
00
7
23
00
00
00
4
00
7
2
56.
1646.
685.
4870.
3695.
91.
1555.
564.
5163.
3505.
7
7
50
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For the Decapoda and Lepomis sp., 95%, i.e., 19 of 20, of the measured tissue
concentrations at Station 2 and 3 were within the 99% confidence limits for the
predicted residues, Table 4-8 and 4-9. For the 95% confidence limits. 90% and 50%
of the observed residues for the Decapoda and Lepomis sp., respectively, were within
their corresponding prediction limits. For the Lepomis sp., chemicals not within their
95% confidence limits were lower than the lower bounds on their confidence limits and
having measured residues lower than the predicted residues is consistent with the
metabolic abilities of these organisms.
4.7 Caged Organisms
The caged organisms, Ictalarus puctatus, were analyzed for the five target
chemicals (Table A-29). Average tissue concentrations for each species were
calculated for Stations 1 and 3 (Table 4-10).
Comparison of the Station 1, upstream of Coke Plant 1, and Station 3,
downstream of Coke Plants 1 and 2, residue concentrations for the caged Ictalarus
puctatus reveals that upstream organisms had higher residue concentrations than the
downstream organisms for the five target chemicals. Also, the tissue concentrations
for the Lepomis sp. and Ictalarus puctatus were quite similar for Station 3 (after
correction for lipid content) and very different for Station 1 (after correction for lipid
content). These results suggested that the upstream cages at Station 1 were
contaminated, had different exposure conditions than the indigenous organisms,
and/or possibly, were mislabeled.
After careful evaluation, no evidence for mislabeling the samples in either the
field or the analytical procedure could be found. In addition, the same type of cages
were used at Stations 1 and 3 which suggests that the cage design was not a factor
for the higher residue concentrations at Station 1.
To evaluate if the different exposure conditions existed at the location of the
cages at Station 1, a set of ambient water samples were collected in December 1990.
Target chemical analyses were performed on the six ambient water samples (Table A-
30). One of the ambient samples, Ambient 4, was taken from a runoff/drainage ditch
which was 140 meters upstream of the dam at Station 1. This ditch and the cages
were on the same creek bank of Five Mile Creek.
The runoff ditch, Ambient 4, contained the highest concentrations for the five
target chemicals all of the ambient water samples, Table A-30. In general, the
ambient water concentrations increased with increasing distance downstream, i.e.,
Ambient 3 < Ambient 5 < Ambient 2 < Ambient 1 < Ambient 6. The ambient
concentrations downstream of the runoff/drainage ditch, Ambients 1 and 2, had higher
concentrations than the upstream stations, Ambients 3 and 5. Note, Ambient 6 was
taken at Station 3 of the site study.
While at the site, we observed that the discharge from the ditch did not mix well
with the reservoir behind the dam. This discharge hugged the creek bank and flowed
51
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Table 4-10. Average Tissue Residues (ppb) in Caged Ictalarus puctatus for Five Mile
Creek.
Station 1 Station 2 Station 3 Station 4
Ictalarus puctatus (Catfish)
Biphenyl
Phenanthrene
Anthracene
Fluoranthene
Pyrene
16.3
109.
12.0
33.0
16.4
(2)a
(2)
(2)
(2)
(2)
3.84
13.3
7.11
16.2
9.72
(2)
(2)
(2)
(2)
(2)
Number of different organism samples analyzed for that station are in
parentheses.
52
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towards the dam following the shore. The cages at Station 1 were near the shore and
in the incompletely mixed water from the ditch. Visual observation of the flow regime
in the creek and conductivity measurements performed on Five Mile Creek were used
to determine that incomplete mixing of the ditch water and reservoir had occurred at
the location of the Station 1 caged exposures.
Further evaluation of the Ictalarus puctatus data was not done due to the lack of
uncontaminated measured tissue residues for Station 1.
The occurrence of the contaminated caged Ictalarus puctatus at Station 1 in the
site study leads to the natural question of "Were the Lepomis sp. and Decapoda
contaminated at Station 1?". For both organisms, we believe that the tissue residues
measured for the organisms for Station 1 were not strongly influenced by the
discharge from the drainage ditch. The Decapoda were sampled at the base of the
dam in much deep water and away from the discharge flow from the ditch. The
Lepomis sp. were sampled away from the shore in deeper water and not in the
incompletely mixed water from the drainage ditch. In addition, the Lepomis sp. were
free ranging and their exposure to the discharge from the ditch would be small since
the near shore part of the reservoir was quite shallow and had no vegetation or cover
for the Lepomis sp. organisms.
4.8 Comparison of the Measured and Predicted Tissue Residues: Unaddressed
Variables
The comparison of the measured and predicted tissue residues in this study
could be influenced by a number of unaddressed variables. In selecting the study
site, a "simple" site was chosen which minimized many of the variables in the study
(section 2). However, some variables and implicit assumptions were beyond control,
e.g., stream and discharge flows. For most of these variables, their importance in the
comparison of the measured and predicted residues was difficult, if not impossible, to
assess.
Probably the most important unaddressed variable in the study was that the
predicted tissue residues were derived by using a model developed from steady-state
exposure conditions. However, the daily in-stream chemical concentrations varied
significantly during the study. For example, anthracene in-stream concentrations
ranged from 0.0 to 77 and 4.3 to 61 ng/l at Stations 2 and 3, respectively, and
changed abruptly 4 or 5 times during the site study. To predict the tissue residues in
the study, the grand means of the daily in-stream concentrations for the length of the
study were used as the best estimator for the exposure concentration for the receiving
water organisms. Other estimators could have been used in this study such as a time
weighted exposure concentration. These estimators might have provided better
predictions with the widely changing exposure concentrations. The exposure
conditions strongly effect the residues predicted and this variable is a source for error.
The measured residue data for the aquatic organisms were implicitly assumed to
be at steady-state/equilibrium with the receiving water. With the dramatic changes in
53
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the daily in-stream concentrations, steady-state conditions were not likely to have
been achieved by the receiving water organisms. The use of tissue data from
receiving organisms not at steady-state conditions is a source of error in this
evaluation.
The residue prediction procedure does not address bioavailability issues
associated with the bioconcentration/bioaccumulation process. At the study site, a
discharge of fines from a upstream quarrying operation was very conspicuous. These
fines may have had an effect on the availability of these chemicals for uptake by the
receiving water organisms. If a significant portion of the chemical was not
bioavailable, the actual exposure conditions would be different than those based on
dilution only.
All of these issues influence the predicted and/or measured tissue residues in the
Decapoda and Lepomis sp. for this study and the importance of each issue is difficult
to evaluate. The most important, possibly, are the issues associated with the steady-
state exposure conditions used in the predictive technique and its relationship to the
widely changing exposure concentrations.
Field studies by their inherent nature can not be controlled like laboratory studies.
Field studies include variables such as those discussed above and allow their
importance/effect in predicting receiving water tissue concentrations to be assessed.
54
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SUMMARY
The objective of the site study was to determine how well tissue residues could
be predicted in field discharge situations using the guidance procedures. For the
Decapoda organisms from Five Mile Creek, the observed and predicted residues
differed by no more than a factor 3 for 9 of the 10 predicted residues. All of the
measured Decapoda tissue residues were within the bounds of the 99% confidence
limits for the predicted residues. For the Lepomis sp., the observed and predicted
residues differed by no more that a factor of 3 for 6 of the 10 predicted residues. For
each chemical, similar agreement between the measured and predicted Lepomis sp.
tissue residues was observed for both sampling stations on Five Mile Creek. For the
caged Ictalarus puctatus, data from these exposures could not be used to evaluate the
residue prediction procedure due to experimental problems.
The chemicals under investigation in this study can be metabolized by aquatic
vertebrates such as fishes. The observed residues in the Lepomis sp. were consistent
with this process. The observed residues were lower than predicted and the more
easily metabolized chemicals had lower observed residues than the less easily
metabolized chemicals. For aquatic invertebrates, metabolism of the five chemicals
under investigation was (or should have been) essentially nonexistent. The data for
the Decapoda organisms were consistent with this metabolic behavior as similar
differences between the measured and predicted residues were observed for all
chemicals at each sampling station.
This study demonstrates that tissue residue concentrations in field discharge
situations can be predicted within a factor of 3 using the developed residue prediction
procedure provided the chemicals are not easily metabolized. When metabolism is
important, residues predicted using the guidance procedure will be too large. The rate
of metabolism will directly influence the difference between the measured and
predicted residues.
The prediction of tissue residues within a factor of 3 for "non-metabolizable"
chemicals, in field discharge situations strongly demonstrates the validity of the
developed residue prediction procedure. Field studies, by their inherent nature,
include the dynamics and variabilities associated with natural aquatic systems. The
predictive ability demonstrated in this site study when considering the variabilities
associated with study, e.g., the widely changing daily in-stream chemical
concentrations, further underscores the capabilities of the prediction technique.
55
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REFERENCES
1. United States Environmental Protection Agency, Office of Water Enforcement
and Permits. Assessment and control of bioconcentratable contaminants in
surface waters. Public Comment Draft (Fed. Regist. 1991, 56, 13150-13151),
March 1991.
2. Veith, G.D. and P. Kosian. 1982. Estimating bioconcentration potential from
octanol/water partition coefficients, in Physical Behavior of PCBs in the Great
Lakes, MacKay, Paterson, Eisenreich, and Simmons, Eds., Chapter 15, Ann
Arbor Science, Ann Arbor, Ml.
3. Schuurmann, G. and W. Klein. 1988. Advances in bioconcentration prediction.
Chemosphere. 17(8): 1551-1574.
4. Thomann, R.V. 1989. Bioaccumulation model of organic chemical distribution
in aquatic food chains. Environ. Sci. Technol. 23:699-707.
5. Thomann, R.V. 1987. A statistical model of environmental contaminants using
variance spectrum analysis. Report to National Science Foundation. August
1987. NTIS #: PB88-235130/A09.
6. Rasmussen, J.B., D.J. Rowan, D.R.S. Lean, and J.H. Carey. 1990. Food
chain structure in Ontario lakes determines PCB levels in lake trout (Salvelinus
namaycush) and other pelagic fish. Can. Journal of Fish. Sci. 47:2030-2038.
7. James, M. O. 1989. Biotransformation and disposition of PAH in aquatic
invertebrates, in Metabolism of Polvcvclic Aromatic Hydrocarbons in the Aquatic
Environment (Varanasi, U., Ed.), CRC: Boca Raton, Florida. Chap 3.
8. Jones, P.A. and R.J. Sloan. 1989. An in situ river exposure vessel for
bioaccumulation studies with juvenile fish. Environ. Toxicol. and Chem. 8:151-
155.
9. Mount, D.I., A.E. Steen, and T.J. Norberg-King. 1985. Validity of effluent and
ambient toxicity testing for predicting biological impact on Five Mile Creek,
Birmingham, AL. EPA/600/8-85/015. U.S. Environmental Protection Agency.
Environmental Research Laboratory, Duluth, MN.
10. Varanasi, U., J.E. Stein, and M. Nishimoto. 1989. Biotransformation and
disposition of polycyclic aromatic hydrocarbon (PAH) in fish, in Metabolism of
Poiycyclic Aromatic Hydrocarbons in the Aquatic Environment (Varanasi, U.,
Ed.), CRC: Boca Raton, Florida. Chap 4.
56
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APPENDIX A
57
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Table A-1. Bioconcentratable Chemicals Tentatively Identified Using the Effluent
Analytical Procedure: Coke Plant 1, Fraction 1.
NBS/EPA/NIH TENTATIVE IDENTIFICATIONS /chem/msd/90a530901011.d
Peak RT Height Amount (ng/L)
(Fit) (Name)
6.132 183520 185.95
83 1,3-Cyclopentadiene, 5-(1-methylethylidene)- (9CI)
80 Benzene, l,2-dimethyl- (9CI)
72 Benzene, ethyl- (8CI9CI)
9.402 287010 336.59
94 Benzene, 1-propynyl- (9CI)
91 Benzene, l-ethynyl-4-methyl- (9CI)
91 Benzene, 1,2-propadienyl- (9CI)
90 IH-Indene (9CI)
' 11.944 1704130 2699.71
97 Azulene (8CI9CI)
90 Naphthalene (ACN)(DOT)(8CI9CI)
83 IH-Indene, 1-methylene- (9CI)
13.673 537907 718.99
90 Naphthalene, 1-methyl- (8CI9CI)
87 Naphthalene, 2-methyl- (8CI9CI)
83 1,4-Methanonaphthalene, 1,4-dihydro- (8CI9CI)
72 IH-Indene, 1-ethylidene- (9CI)
13.921 280745 327.04
93 IH-Indene, 1-ethylidene- (9CI)
91 Naphthalene, 1-methyl- (8CI9CI)
91 Naphthalene, 2-methyl- (8CI9CI)
87 1,4-Methanonaphthalene, 1,4-dihydro- (8CI9CI)
14.SS9 232018 252.77
90 l,l'-Biphenyl (9CI>
87 Naphthalene, 2-ethenyl- (9CI)
15.888 648837 888.06
91 Acenaphthylene (8CI9CI)
16.682 901367 1311.00
94 Phenol, 2,6-bis(1,l-dimethylethyl)-4-methyl- (9CI)
16.780 810408 1153.65
83 Dibenzofuran (8CI9CI)
17.709 1122900 1694.24
87 9H-Fluorene (9CI)
80 9H-Fluorene-9-carboxylic acid (9CI)
18.270 119301 123.96
72 9H-Fluorene-9-carboxylic acid (9CI)
18.394 130809 135.07
94 Dibenzofuran, 4-methyl- (8CI9CI)
A-1
-------
Table A-1. continued.
20.737 1914910 3064.34
97 9H-Fluorene, 9-methylene- (9CI)
91 Benzene, 1,1'-(1,2-ethynediyl)bis- (9CI)
87 Anthracene (8CI9CI)
78 2-Cyclopropen-l-one, 2,3-diphenyl- (9CI)
74 Phenanthrene (8CI9CI)
20.839 1159380 1757.34
94 9H-Fluorene, 9-methylene- (9CI)
20.169 447845 581.72
72 1,1'-Biphenyl, 2-methoxy- (9CI)
20.737 1914910 3064.34
97 9H-Fluorene, 9-methylene- (9CI)
91 Benzene, 1,1'-(1,2-ethynediyl)bis- (9CI)
87 Anthracene (8CI9CI)
78 2-Cyclopropen-l-one, 2,3-diphenyl- (9CI)
74 Phenanthrene (8CI9CI)
20.839 1159380 1757.34
94 9H-Fluorene, 9-methylene- ( 9CI)
91 Benzene, 1,1'-(1,2-ethynediyl)bis- (9CI)
72 2-Cyclopropen-l-one, 2,3-diphenyl- (9CI)
22.606 113068 117.94
97 9H-Fluorene-2-carbonitrile (9CI)
25.679 123128 127.65
95 9-Anthracenecarbonitrile (9CI)
26.090 181249 183.76
91 Heptadecanoic acid, 15-methyl-, methyl ester (9CI)
83 Octadecanoic acid, methyl ester (9CI)
81 Pentadecanoic acid, methyl ester (8CI9CI)
72 9-Octadecenoic acid, 12-(acetyloxy)-, methyl ester, [R-(Z)]- (9CI)
72 2-Naphthalenol, 8-amino- (9CI)
A-2
-------
Table A-2. Bioconcentratable Chemicals Tentatively Identified Using the Effluent
Analytical Procedure: Coke Plant 1, Fraction 2.
NBS/EPA/NIH TENTATIVE IDENTIFICATIONS
/chem/msd/90a531001012.d
Peak
15.
15.
15.
16.
16.
16-.
16.
RT
(Fit)
261
97
97
96
96
95
95
95
95
91
90
472
97
96
96
95
94
93
93
87
76
72
70
70
516
93
69
69
69
76
70
100
99
680
96
890
93
93
93
91
953
91
90
90
87
Height
(Name)
143304
Naphthalene,
Naphthalene,
Naphthalene,
Naphthalene,
Naphthalene,
Naphthalene ,
Naphthalene,
Naphthalene,
Naphthalene,
Naphthalene,
126182
Naphthalene,
Naphthalene,
Naphthalene,
Naphthalene,
Naphthalene,
Naphthalene ,
Naphthalene,
Naphthalene,
Naphthalene,
Naphthalene ,
Naphthalene,
Naphthalene,
88561
Naphthalene ,
Naphthalene,
Naphthalene ,
Naphthalene,
Naphthalene,
Naphthalene,
194957
Amount ( ng/L )
211.62
1.7-dimethyl- (8CI9CI)
2,6-dimethyl- (8CI9CI)
1,6-dimethyl- (8CI9CI)
1,8-dimethyl- (8CI9CI)
1 , 3-dimethyl- (8CI9CI)
1,5-dimethyl- (8CI9CI)
2,7-dimethyl- (8CI9CI)
2, 3-dimethyl- (8CI9CI)
1,2-dimethyl- (8CI9CI)
1,4-dimethyl- (8CI9CI)
187.66
1,6-dimethyl- (8CI9CI)
2, 3-dimethyl- (8CI9CI)
1,8-dimethyl- (8CI9CI)
1,4-dimethyl- (8CI9CI)
1,7-dimethyl- (8CI9CI)
1,2-dimethyl- (8CI9CI)
1, 3-dimethyl- (8CI9CI)
2-ethyl- (8CI9CI)
1,5-dimethyl- (8CI9CI)
1-ethyl- (8CI9CI)
2,6-dimethyl- (8CI9CI)
2,7-dimethyl- (8CI9CI)
135.66
2,7-dimethyl- (8CI9CI)
1,6-dimethyl- (8CI9CI)
2,6-dimethyl- (8CI9CI)
1,7-dimethyl- (8CI9CI)
1,5-dimethyl- (8CI9CI)
1 , 3-dimethyl- (8CI9CI)
312.30
2,5-Cyclohexadiene-l,4-dione, 2, 6-bis( 1 ,1-dimethylethyl)- (9CI)
679136
1429.58
Phenol, 2,6-bis(l,l-dimethylethyl)-4-methyl- (9CI)
66374
Naphthalene,
Naphthalene,
Naphthalene,
Naphthalene,
90725
Naphthalene,
Naphthalene,
Naphthalene,
Naphthalene,
104.87
1,4,6-trimethyl- (8CI9CI)
1,6,7-trimethyl- (8CI9CI)
2,3,6-trimethyl- (8CI9CI)
l,4,5-trin»thyl- (8CI9CI)
138.66
2,3,6-trimethyl- (8CI9CI)
1,4,5-trimethyl- (8CI9CI)
1,6,7-trimethyl- (8CI9CI)
1,4,6-trimethyl- (8CI9CI)
A-3
-------
Table A-2. continued.
17.173 83064 128.03
98 Naphthalene. 1,4,6-trimethyl- (8CI9CI)
98 Naphthalene, 1,4.5-trimethyl- (8CI9CI)
97 Naphthalene, 1,6,7-trimethyl- (8CI9CI)
95 Naphthalene, 2,3,6-trimethyl- (8CI9CI)
17.407 64300 102.00
97 Naphthalene. 1,4,6-trimethyl- r8CI9CI)
97 Naphthalene, 1,6,7-trimethyl- (8CI9CI)
97 Naphthalene, 2,3,6-trimethyl- (8CI9CI)
96 Naphthalene, 1.4,5-trimethyl- (8CI9CI)
18.208 240205 414.20
90 Dibenzofuran, 4-methyl- (8CI9CI)
16.401 207488 340.52
94 Dibenzofuran, 4-methyl- 8CI9CI)
16.9:2 74735 116.48
93 Azulene, 7-ethyl-l,4-dimethyl- (8CI9CI)
91 Naphthalene, l-methyl-7-(l-methylethyl)- (9CI)
19.350 2753^8 493.41
93 9H-Fluor
-------
Table A-2. continued.
22.070 180863 280.56
93 Dibenzothiophene, 3-methyl- (8CI9CI)
83 Dibenzothiophene, 4-methyl- (8CI9CI)
22.441 494623 987.14
93 Anthracene, 1-methyl- (8CI9CI)
93 Phenanthrene, 4-methyl- (8CI9CI)
87 IH-Indene, 1-phenyl- (9CI)
87 Anthracene, 2-methyl- (8CI9CI)
87 Phenanthrene, 1-methyl- (8CI9CI)
80 9H-Fluorene, 9-ethylidene- (9CI)
80 IH-Indene, 2-ph«nyl- (9CI)
80 Phenanthrene, 3-methyl- (8CI9CI)
80 Phenanthrene, 9-methyl- (6CI9CI)
22.551 639218 1328.64
93 Anthracene, 2-methyl- (8CI9CI)
87 IH-Indene, 1-phenyl- (9CI)
83 Phenanthrene, 4-m*thyl- (8CI9CI)
81 Phenanthrene, 3-methyl- (8CI9CI)
81 Phenanthrene, 1-methyl- (8CI9CI)
76 IH-Indene, 2-phenyl- (9CI)
72 9H-Fluorent, 9-ethylidene- (9CI)
22.703 203799 332,21
96 Phenanthrene, 4-methyl- (8CI9CI)
91 Anthracene, 1-methyl- (8CI9CI)
90 Phenanthrene, 2-methyl- (8CI9CI)
90 Anthracene, 2-methyl- (8CI9CI)
87 Phenanthrene, 3-methyl- (8CI9CI)
87 Phenanthrene, 1-methyl- (8CI9CI)
80 IH-Indene, 2-phenyl- (9CI)
74 IH-Indene, 1-phenyl- {9CI)
72 Phenanthrene, 9-methyl- (8CI9CI)
22.959 472102 936.43
94 Anthracene, 1-methyl- (8CI9CI)
90 Anthracene, 2-methyl- (8CI9CI)
87 Phenanthrene, 3-methyl- (8CI9CI)
81 IH-Indene, 1-phenyl- (9CI)
80 IH-Indene, 2-phenyl- (9CI)
74 Phenanthrane, 4-methyl- (8CI9CI)
72 Phenanthrene, 2-methyl- (8CI9CI)
23.394 166108 247.33
78 Naphtho[2,3-b]thiophene, 4,9-dimethyl- (8CI)
23.665 473879 940.43
80 Naphthalene, 2-phenyl- (8CJ9CI)
24.238 110229 165.73
91 Phenanthrene, 2,3-dimethyl- (8CI9CI)
90 Phenanthrene, 4,5-dimethyl- (8CI9CI)
81 Phenanthrene, 2-ethyl- (8CI9CI)
76 Phenanthrene, 2,5-dimethyl- (8C19CI)
A-5
-------
Table A-2. continued.
24,408
91
86
70
24,478
81
24.794
83
2S.49S
93
25.694
§5
80
74
26.458
94
76
26.594
81
26.822
96
27, 315
90
87
1^.343
81
81
81
81
74
81
76
70
2*. 605
89
61
76
28.721
87
87
81
156439 229.85
Phenanthrene, 2,5-dimethyl- (8CI9CI)
Phenanthrene , 3,6-dimethyl- (8CI9CI)
Phenanthrene, 2,7-dimethyl- (8CI9CI)
104224 157.39
Phenanthrene, 2,7-dimethyl- (8CI9CI)
177122 272.14
Ph«nanthr«ne , 2,7-dimethyl- (8CI9CI)
1903910
4S2S.76
(8CI9CI)
507242 1015.56
Pluoranttuna (8CI9CI)
Benzene, 1.1 '-( 1,3-butadiyne-l ,4-diyl)bis- (9CI)
----- -(8CI9CI)
1867360 4434.33
Pyrm* (8CI9CI)
Fluoranthene (8CI9CI)
385418 741.22
Benzo[b]naphtho[2,3-d]furan (8CI9CI)
530465 1067.86
Benzolb]naphtho[2,3-d]furan (8CI9CI)
650297 1356.6S
llH-Benzo[b]fluorene (8CI9CI)
HH-Benzo[a]fluorene (8CI9CI)
§15614 2027.58
HH-BenzoU]fluorene (8CI9CI)
::H-Benzo{b]fluorene (8CI9CI)
761893 1638.85
Pyrene, 4-methyl- (8CI9CI)
HH-Benzo[a]fluorene (8CI9CI)
Pyr«?ne, 1-mtthyl- (8CI9CI)
Pyrene, 2-methyl- (8CI9CI)
935136 1824.07
Pyrene, 2-me»-.hyl- (8CI9CI)
Pyrene, :-methyl- <8CI9CI!
Pyrene,,4-methyi- (8CI9CI)
•594607 1468.70
Pyrene, 2-methyl- (8CI9CI)
Pyrene, 1-methyl- (bC!9CI)
Pyrene, 4-methyl- (8CI9CI
S84954 1191.42
HH-Benzo[b]fluorene (SCIaCI)
Pyrene, l-metnyl- (SCISCIj
HH-Benzo(a]fluorene (8CI9CI)
A-6
-------
Table A-2. continued.
29.265
93
72
29.425
78
30.532
96
94
30.798
93
30.901
98
96
31.152
97
94
31.227
90
81
32.059
97
93
91
76
32.313
90
81
81
74
32.677
70
70
70
70
33.407
93
91
91
89
61
81
104178 157.33
1,1':2',1" -Terphenyl (9CI)
Pyrene, 1,3-dimethyl- (9CI)
80283 124.17
Pyrene, 1,3-dimethyl- (9CI)
489954 976.63
Benzo[b]naphtho[2.1-d]thiophene (8CI9CI)
Benzo[b]naphtho[1,2-d]thiophene (8CI9CI)
1068460 2414.09
Benzo(ghi]fluoranthene (8CI9CI)
361217 686.72
Benzo[b]naphtho[2,l-d]thiophene (8CI9CI)
Benzolb]naphtho[1,2-d]thiophene (8CI9CI)
176891 271.62
Benzo[b]naphtho[l,2-d]thiophene (8CI9CI)
Benzolb]naphtho[2,1-d]thiophene (8CI9CI)
361056 686.35
Benzolb]naphtho[2,l-d]thiophene (8CI9CI)
Benzo[b]naphtho[l,2-d]thiophene (8CI9CI)
1393330 3235.62
Triphenylene (8CI9CI)
Benzo[c]phenanthrene (8CI9CI)
Chrysene (8CI9CI)
Naphthacene (8CI9CI)
144968 213.93
Benzo[aIpyrene, 4,5-dihydro-
l,2'-Binaphthalene (9CI)
Anthracene, 9-phenyl- (8CI9CI)
l,l'-Binaphthaiene (9CI)
575109 1168,
Benz[a]anthracene,
Benz[a]anthracene,
Benz[a]anthracene,
Benz[a]anthracene,
40
4-methyl- (8CI9CI)
9-methyl- (8CI9CI)
10-methyl- (8CI9CI)
11-methyl- (8CI9CI)
176196 270.05
Benz[a]anthracene, 1-methyl- (8CI9CI)
Chrysene, 5-methyl- (8CI9CI)
Chrysene, 4-methyl- (8CI9CI)
Benz[a]anthracene, 12-methyl- (8CI9CI)
Benz[a]anthracene, 7-methyl- (8CI9CI)
Triphenylene, 2-methyl- (8CI9CI)
A-7
-------
Table A-2. continued.
33.639 615697 1269.16
98 Benz[a]anthracene, 1-methyl- (8CI9CI)
97 Benz[aJanthracene, 12-methyl- (8CI9CI)
97 Chrysene, 5-mcthyl- (8CI9CI)
96 Triphenylene, 2-methyl- (8CI9CI)
95 Chrysene, 4-methyl- (8CI9CI)
91 Chrysene, 6-methyl- (8CI9CD
90 Benz[a]anthracene, 2-methyl- (8CI9CI)
89 Benz[a]anthracene, 8-methyl- (8CI9CI)
89 Chrysene, 3-methyl- (8CI9CI)
89 Benz[a]anthracene, 7-methyl- (8CI9CI)
89 Chrysene, 1-methyl- (8CI9CI)
81 Benz[aJanthracene. 5-methyl- (8CI9CI)
81 Benz[a]anthracene, 6-methyl- (8CI9CI)
81 Benz[aJanthracene, 3-methyl- (8CI9CI)
81 Benz[aJanthracene, 4-methyl- (8CI9CI)
76 Benzo[c]phenanthrene, 6-methyl- (8CI9CI)
76 Benzofciphenanthrene. 5-methyl- (8CI9CI)
76 Benz[a]anthracene, ll-methyl- (8CI9CI)
70 Benzo[c]phenanthrene, 3-methyl- (8CI9CI)
33.791 431246 844.42
92 ' Benz[a]anthracene, 1-methyl- (8CI9CI)
86 Chrysene, 4-methyl- (8CI9CI)
83 Benzo[c]phenanthrene, 6-methyl- (8CI9CI)
83 Benzo[c]phenanthrene, 5-methyl- (8CI9CI)
70 Chrysene, 5-methyl- (8CI9CI)
70 Benz[a]anthracene, 7-methyl- (8CI9CI)
34.627 246755 428.95
87 2,2'-Binaphthalene (9CI)
34.802 79481 123.06
87 2,2'-Binaphthalene (9CI)
35.111 72493 113.36
93 Benzo[a]pyrene, 4,5-dihydro-
89 9H-Fluorene, 9-(phenylmethylene)- (9CI)
76 l,l'-Binaphthalene (9CI)
36.735 1330180 3075.93
98 Benzoljjtluoranthene (8CI9CI)
98 Benz[e]acephenanthrylene (8CI9CI)
95 Benzo[e]pyrene (8CI9CI)
94 Benzo[Jc)tluoranthene (8CI9CI)
94 Benzo[a]pyrene (8CI9CI)
37.041 796934 1727.47
94 Benzo[e]pyrene (8CI9CI)
89 Benz[e]acephenanthrylene (8CI9CI)
8i Benzo[k]fluoranthene (8CI9CI)
76 Benzo[j]fluoranthene (8CI9CI)
70 Benzo[a]pyrene (8CI9CI)
A-8
-------
Table A-2. continued.
37.188 161686 237.38
78 l,l'-Binaphthalene, 3,3'.4.4'-tetrahvdro- (9CI)
37.871 1200820 2748.80
98 Benzo[e]pyrene (8CI9CI)
96 Benzofj]fluoranthene (8CI9CI)
96 Benz[e]acephenanthrylene (8CI9CI)
86 Benzofajpyrene (8CI9CI)
76 Benzo[k]fluoranthene (8CI9CI)
38.044 973206 2173.22
95 Benzo[j]fluoranthene (8CI9CI)
94 Benzo[ajpyrene (8CI9CI)
93 Benzo[e]pyrene (8CI9CI)
38.253 318810 591.22
95 Benzofj]fluoranthene (8CI9CI)
95 Benzo[k]fluoranthene (8CI9CI)
95 Benz[e]acephenanthrylene (8CI9CI)
93 Benzo[e]pyrene (8CI9CI)
A-9
-------
Table A-3. Bioconcentratable Chemicals Tentatively Identified Using the Effluent
Analytical Procedure: Coke Plant 2, Fraction 1.
NBS/EPA/NIH TENTATIVE IDENTIFICATIONS /chem/msd/90a78090l009.d
Peak RT Height Amount (ng/L)
(Fit) (Name)
16.279 596946 195.6-9
94 Acenaphthene (SCI)
16.610 2768910 1103.14
96 Phenol, 2,6-bis(l,l-dimethylethyl)-4-n»thyl- (9CI)
16.696 495917 164.57
72 Benzenemethanol, 3,4-dimethoxy- (9CI)
17.599 730106 242.22
90 9H-Fluorene-9-carboxylic acid (9CI)
90 9H-Fluorene (9CI)
25.966 592929 194.45
94 Heptadecanoic acid, 14-methyl-. methyl ester, (.+-.)- (9CZ)
91 Heptadecanoic acid, 14-methyl-, methyl ester (9CI)
67 Heptadecanoic acid, 15-methyl-, methyl ester (9CI)
86 Pentadecanoic acid, methyl ester (8CI9CI)
86 Heptadecanoic acid, 16-methyl-, methyl ester (8CI9CI)
76 Heptadecanoic acid, 10-methyl-, methyl ester (9CI)
78 Octadecanoic acid, methyl ester (9CI)
32.802 290054 101.16
87 l,2-Benzenedicarboxylic acid, 3-nitro- (9CI)
A-10
-------
Table A-4. Bioconcentratable Chemicals Tentatively Identified Using the Effluent
Analytical Procedure: Coke Plant 2, Fraction 2.
NES/EPA/NIH TENTATIVE IDENTIFICATIONS
/cheru/msd/90a7Bl00101 n .d
RT
(Fit)
8.995
87
63
83
83
78
72
72
72
72
72
16.032
99
16.608
93
19.351
81
76
76
70
22.663
80
25.041
95
25.894
87
81
25.982
83
83
80
74
Height
(Name;
Amount (r.g/L)
361495 173.43
Benzene, 1.2,3,5-tetramethyl- (8CI9CI)
Benzene, l-methyl-3-(1-methylethyl)- (9CI)
Benzene, methyl(1-methylethyl)- (9CI)
Benzene, l-methyl-2-(1-methylethyl)- (9CI)
1,4-Cyclohexadiene, 3-ethenyl-l,2-dimethyl- (9CI)
Benzene, 2-ethyl-l,4-dimethyl- (9CI)
Benzene, l-ethyl-3,5-dimethyl-
Benzene, 2-ethyi-l,3-dimethyl-
Benzene, l-ethyl-2,4-dimethyl-
(9CI)
(9CI)
(9CI)
Benzene, 4-ethyl-l,2-dimethyl- (9CI)
1050520 542.74
2,5-Cyclohexadiene-l,4-dione, 2,6-bis(1,1-dimethylethyl)- (9CI)
1746700 962.22
Phenol, 2/6-bis(l,l-dimethylethyl)-4-methyl- (9CI)
198626
9H-Fluorene,
9H-Fluorene,
9H-Fluorene,
100.63
4-methyl- (9CI)
1-methyl- (9CI)
2-methyl- (9CI)
9H-Fluorene, 9-methyl- (9CI)
472215 222.92
4H-Cyclopenta[defJphenanthrene (8CI9CI
1200400 633.05
Fluoranthene (8CI9CI)
587394 274.40
Fluoranthene (8CI9CI)
Pyrene (8CI9CI)
279542 136.80
Heptadecanoic acid, 14-methyl-,
Heptadecanoic acid, 14-methyl-,
Heptadecanoic acid, 16-methyl-,
methyl ester,
methyl ester
methyl eater
Heptadecanoic acid, 15-methyl-, methyl ester
(.«•-.)- (9CI)
(9CI)
(8CI9CI)
(9CI)
A-11
-------
Table A-5. Bioconcentratable Chemicals Tentatively Identified Using the Effluent
Analytical Procedure: Coke Plant 2, Fraction 3.
NBS/EPA/NIH TENTATIVE IDENTIFICATIONS /chem/msd/90a781101011 .d
Peak RTHeightAmount (ng/L)
(Fit) (Name)
16.597 3140390 1612.96
94 Phenol, 2,6-bis(1,1-dimethylethyl)-4-methyl- (9CI)
22.656 330255 121.45
83 Docosane (8CI9CI)
80 Heptacosane (8CI9CI)
80 Heptadecane, 9-octyl- (8CI9CI)
72 Tetradecane, 2-methyl- (8CI9CI)
32.805 640152 242.07
72 1,2-Benzenedicarboxylic acid, diisooctyl ester (9CI)
A-12
-------
Table A-6. Measured and Predicted Stream Flows at Three Locations on Five Mile
Creek, Jefferson County, Alabama.
Measured Flow (m3/s)
Date Station 1" Station 3
March 26
27
28
29
30
31
.April 1
2
3
4
5
6
7 0.872
8 1.045
9
10
1 1 0.767 0.988
12 0.646
13
14
15 0.708 0.929
16
17
18
19 0.943
20
21 0.677 0.813
22
23 0.790
24
25
26
Average Flow
Standard Deviation
Coefficient of Variation, %
Republic"
2.24
2.07
1.93
1.84
2.15
1.98
1.76
1.67
1.61
1.53
1.53
1.76
1.76
1.42
1.39
1.67
1.67
1.36
1.36
1.33
1.44
1.27
1.27
1.22
1.19
1.22
1.16
1.22
1.13
1.39
1.10
1.10
1.52
0.316
20.8
Predicted Flow (m3/s)
Station 1a
0.979
0.924
0.879
0.851
0.952
0.897
0.824
0.796
0.778
0.750
0.750
0.824
0.824
0.714
0.705
0.796
0.796
0.696
0.696
0.686
0.723
0.668
0.668
0.650
0.641
0.650
0.631
0.650
0.622
0.705
0.613
0.613
0.748
0.102
13.7
Station 3
1.223
1.165
1.117
1.089
1.194
1.137
1.060
1.031
1.012
0.983
0.983
1.060
1.060
0.945
0.935
1.031
1.031
0.926
0.926
0.916
0.955
0.897
0.897
0.878
0.868
0.878
0.859
0.878
0.849
0.935
0.840
0.840
0.981
0.107
10.9
• 50 m downstream of Station 1.
b USGS gage located at Republic, Alabama.
A-13
-------
Table A-7. Regression Equations for Predicting Stream Flows at Stations 1 and 3
and Predicted Stream Flows with 95% Confidence and Prediction
Intervals for Selected Flows for Stations 1 and 3 for Five Mile Creek.
Station 1
Predicted Flow = 0.2563 + 0.3232 (Measured Flow at Republic)
Standard Deviation of Slope = 0.1074
Standard Deviation of Constant = 0.2563
r2 = 0.7512
degrees of freedom = 4
Measured
Flow
1.00
1.25
1.50
1.75
2.00
2.25
Predicted
Flow
0.5795
0.6602
0.7410
0.8218
0.9026
0.9834
95% Confidence
Interval
0.4004
0.5533
0.6674
0.7035
0.7098
0.7097
0.7585
0.7672
0.8147
0.9401
1.0954
1.2571
95% Prediction
Interval
0.3367
0.4646
0.5614
0.6197
0.6496
0.6644
0.8222
0.8559
0.9207
1.0239
1.1556
1.3024
Station 3
Predicted Flow = 0.4663 + 0.3381 (Measured Flow at Republic)
Standard Deviation
Standard Deviation
r2 =
degrees of freedom
Measured
Flow
of
of
Predicted
Flow
Slope =
Constant
95%
=
0.1627
0.2196
0.5191
5
Confidence
Interval
1.00
1.25
1.50
1.75
2.00
2.25
0.8045
0.8890
0.9735
1.0581
1.1426
1.2271
0.6295
0.7936
0.8589
0.8515
0.8299
0.8048
0.9794
0.9844
1.0881
1.2646
1.4552
1.6494
95% Prediction
Interval
0.5283
0.6550
0.7311
0.7609
0.7639
0.7538
1.0806
1.1230
1.2160
1.3553
1.5213
1.7004
A-14
-------
Table A-8. Daily Effluent Flow for Coke Plant 1 and Coke Plant 2.
Date
March 26
27
28
29
30
31
April 1
2
3
4
5
6
7
8
9
10
Flow (m3/s)
Coke Coke
Plant 1 Plant 2
0.00358 0.175
0.00615 0.162
0.00599 0.175
0.00677 0.197
0.00441 0.188
0.00372 0.171
0.00400 0.188
0.00386 0.188
0.00439 0.171
0.00799 0.180
0.00499 0.188
0.00814 0.188
0.00128 0.149
0.00467 0.149
0.00593 0.180
0.00529 0.223
Average discharge flow
Standard Deviation
Coefficient of Variation,
Date
April 1 1
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
%
Flow
Coke
Plant 1
0.00457
0.00413
0.00612
0.00792
0.00755
0.00680
0.00509
0.00188
0.00000
0.00000
0.00338
0.00587
0.00522
0.00426
0.00395
0.00493
0.00478
0.00201
42.1
fm'/s)
Coke
Plant 2
0.184
0.180
0.175
0.166
0.175
0.197
0.197
0.188
0.188
0.193
0.180
0.184
0.202
0.202
0.202
0.188
0.184
0.0150
8.16
A-15
-------
Table A-9. In-stream Effluent Concentrations for Discharges from Coke Plants 1 and
2.
Date
Predicted
Stream Flow
Station 1 Station 3
m3/s m3/s
Discharge Flow
Plant 1 Plant 2
m3/s m3/s
In-Stream
Effluent
Concentration
Plant 1 Plant 2
March 26
27
28
29
30
31
April 1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
0.979
0.924
0.879
0.851
0.952
0.897
0.824
0.796
0.778
0.750
0.750
0.824
0.824
0.714
0.705
0.796
0.796
0.696
0.696
0.686
0.723
0.668
0.668
0.650
0.641
0.650
0.631
0.650
0.622
0.705
0.613
0.613
1.223
1.165
1.117
1.089
1.194
1.137
1.060
1.031
1.012
0.983
0.983
1.060
1.060
0.945
0.935
1.031
1.031
0.926
0.926
0.916
0.955
0.897
0.897
0.878
0.868
0.878
0.859
0.878
0.849
0.935
0.840
0.840
average
0.00358
0.00615
0.00599
0.00677
0.00441
0.00372
0.00400
0.00386
0.00439
0.00799
0.00499
0.00814
0.00128
0.00467
0.00593
0.00529
0.00457
0.00413
0.00612
0.00792
0.00755
0.00680
0.00509
0.00188
0.00000
0.00000
0.00338
0.00587
0.00522
0.00426
0.00395
0.00493
0.175
0.162
0.175
0.197
0.188
0.171
0.188
0.188
0.171
0.180
0.188
0.188
0.149
0.149
0.180
0.223
0.184
0.180
0.175
0.166
0.175
0.197
0.197
0.188
0.188
0.193
0.180
0.184
0.202
0.202
0.202
0.188
standard deviation
coefficient
of variation, %
0.365
0.666
0.682
0.795
0.464
0.414
0.485
0.485
0.564
1 .064
0.666
0.988
0.155
0.654
0.842
0.664
0.574
0.594
0.880
1.153
1.044
1.017
0.762
0.290
0.000
0.000
0.535
0.903
0.838
0.605
0.644
0.805
0.644
0.282
43.9
14.3
13.9
15.7
18.1
15.8
15.0
17.8
18.3
16.9
18.3
19.2
17.8
14.1
15.8
19.2
21.7
17.8
19.4
18.9
18.2
18.4
22.0
22.0
21.5
21.7
22.0
20.9
21.0
23.7
21.5
24.0
22.4
19.0
2.80
15.8
A-16
-------
Table A-10. Coke Plant 1 Weekly Effluent Composite for Target Analysis on Biphenyl.
Week 1 Week 2 Week 3 Week 4
A. Effluent Concentrations (ppb, recovery corrected)
Battelle 0.44 0.68
0.40 0.66
ERL-D 0 0 3.34 0
0 0 3.35 0
B. Blank Concentrations (ppb, recovery corrected)
Battelle 0.02 0
ERL-D 0 0 00
C. Surrogate Recoveries
Effluent
Battelle
ERL-D
Blank
Battelle 82.0 64.0
ERL-D 74.0 102.8 76.9 98.3
45.0
48.3
91 .8
97.3
66.0
55.4
74.7
73.4
44.4
42.2
87.4
88.7
A-17
-------
Table A-11. Coke Plant 1 Weekly Effluent Composite for Target Analysis on
Phenanthrene.
Week 1 Week 2 Week3 Week 4
A. Effluent Concentrations (ppb, recovery corrected)
Battelle 16.0 24.5
14.9 20.3
ERL-D 15.9 12.0 15.9 10.1
17.2 13.4 14.0 7.19
B. Blank Concentrations (ppb, recovery corrected)
Battelle 0.12 0.14
ERL-D 0000
C. Surrogate Recoveries
Effluent
Battelle
ERL-D
Blank
Battelle 86 74
ERL-D 76.6 106.2 84.9 99.0
48.9
54.4
97.4
96.7
56.0
49.4
79.3
80.1
46.7
52.6
86.2
69.4
A-18
-------
Table A-12. Coke Plant 1 Weekly Effluent Composite for Target Analysis on
Anthracene.
Week 1 Week 2 Week 3 Week 4
A. Effluent Concentrations (ppb, recovery corrected)
Battelle 6.64 12.0
6.15 8.33
ERL-D 7.17 . 4.19 7.31 4.89
7.36 4.71 6.64 3.94
B. Blank Concentrations (ppb, recovery corrected)
Battelle 0.01 0.14
ERL-D 0000
C. Surrogate Recoveries
Effluent
Battelle
ERL-D
Blank
Battelle 88.0 84.0
ERL-D 70.0 103.9 74.2 95.3
53.7
57.6
95.5
92.2
66.0
53.0
75.3
79.1
55.6
74.1
88.2
90.0
A-19
-------
Table A-13. Coke Plant 1 Weekly Effluent Composite for Target Analysis on
Fluoranthene.
Week 1 Week 2
A. Effluent Concentrations (ppb, recovery corrected)
Battelle
ERL-D 19.7 16.4
22.5 18.4
B. Blank Concentrations (ppb, recovery corrected)
Battelle
ERL-D 0 0
C. Surrogate Recoveries
Effluent
Battelle
Weeks
25.9
22.1
20.5
17.4
0.08
0
66.0
57.0
Week 4
25.1
28.2
18.9
13.7
0.05
0
86.7
70.7
ERL-D
Blank
Battelle 92.0 78.0
ERL-D
* d,0-Pyrene recoveries used for recovery correction.
A-20
-------
Table A-14. Coke Plant 1 Weekly Effluent Composite for Target Analysis on Pyrene.
Week 1 Week 2
A. Effluent Concentrations (ppb, recovery corrected)
Battelle
ERL-D 13.5 11.3
15.4 12.8
B. Blank Concentrations (ppb, recovery corrected)
Battelle
ERL-D 0 0
C. Surrogate Recoveries
Effluent
Battelle
ERL-D 64.9 102.8
71.5 98.6
Blank
Battelle
ERL-D 79.7 109.2
Week 3
19.2
17.0
14.4
12.3
0.03
0
68.0
56.7
84.8
87.5
94.0
79.5
Week 4
21.9
23.1
14.2
10.6
0.03
0
82.2
70.0
96.9
98.4
74.0
100.1
A-21
-------
Table A-15. Coke Plant 2 Weekly Effluent Composite for Target Analysis on Biphenyl.
Week 1 Week 2
Week 3
Week 4
A. Effluent Concentrations (ppb, recovery corrected)
Battelle
ERL-D
B. Blank
Battelle
ERL-D
0
0
0
0.00695
Concentrations (ppb, recovery
0.00
._
0.03
0.05
0.0407
0.0410
corrected)
0.00
0
0.04
0.05
0.04
0.05
0.0567
0.0577
0.02
0
0.06
0.09
0.0508
0.0508
0.00
0
C. Surrogate Recoveries
Effluent
Battelle
ERL-D
Blank
Battelle
ERL-D
77.8
78.3
102.2
103.3
66.7
_ _
88.9
71.6
93.4
92.3
100.0
101.1
73.3
65.5
74.0
59.2
102.2
81.3
82.0
87.9
51.1
63.7
84.6
75.8
64.0
83.5
A-22
-------
Table A-16. Coke Plant 2 Weekly Effluent Composite for Target Analysis on
Phenanthrene.
Week 1
Week 2
Week 3
Week 4
A. Effluent Concentrations (ppb, recovery corrected)
Battelle
ERL-D
0.08
0.07
0.0208
0.0214
B. Blank Concentrations (ppb, recovery
Battelle
ERL-D
C. Surrogate
Effluent
Battelle
ERL-D
Blank
Battelle
ERL-D
0.06
--
Recoveries
77.8
84.9
94.5
102.2
66.7
..
0.09
0.08
0.0621
0.0594
corrected)
0.08
0
88.9
85.1
98.9
98.9
100.0
85.7
0.14
0.13
0.19
0.20
0.117
0.123
0.12
0
77.8
75.0
68.0
65.8
105.5
93.4
86.0
85.7
0.14
0.81
0.0588
0.0670
0.14
0
64.4
88.9
95.6
79.1
74.0
84.6
A-23
-------
Table A-17. Coke Plant 2 Weekly Effluent Composite for Target Analysis on
Anthracene.
Week 1 Week 2
Week 3
Week 4
A. Effluent Concentrations (ppb, recovery corrected)
Battelle
ERL-D
B. Blank
Battelle
ERL-D
0.02
0.02
0.0180
0.0191
Concentrations (ppb, recovery
0.00
..
0.03
0.02
0.0361
0.0327
corrected)
0.11
0
0.02
0.03
0.04
0.04
0.0310
0.0304
0.01
0
0.04
0.09
0.0336
0.0349
0.14
0
C. Surrogate Recoveries
Effluent
Battelle
ERL-D
Blank
Battelle
ERL-D
88.9
98.9
84.3
91.6
77.8
..
88.9
89.2
92.8
95.2
77.8
73.5
91.1
89.6
76.0
75.4
114.5
109.6
88.0
88.0
77.8
104.4
92.8
83.1
84.0
78.3
A-24
-------
Table A-18. Coke Plant 2 Weekly Effluent Composite for Target Analysis on
Fluoranthene.
Week 1 Week 2
Weeks
Week 4
A. Effluent Concentrations (ppb, recovery corrected)
Battelle
0.18
0.19
ERL-D 0.227
0.228
B. Blank Concentrations (ppb, recovery
Battelle 0.04
ERL-D
C. Surrogate Recoveries
Effluent
Battelle
88.9
90.6
0.25
0.22
0.275
0.280
corrected)
0.05
0
88.9
81.8
0.29
0.29
0.35
0.36
0.216
0.219
0.08
0
91.1
83.7
74.0
71.5
0.31
0.42
0.257
0.247
0.05
0
64.4
90.7
ERL-D
Blank
Battelle 77.8 111.1 92.0 78.0
ERL-D
• d10-Pyrene recoveries used for recovery correction.
A-25
-------
Table A-19. Coke Plant 2 Weekly Effluent Composite for Target Analysis on Pyrene.
Week 1 Week 2 Week 3 Week 4
A. Effluent Concentrations (ppb, recovery corrected)
Battelle
0.10
0.10
0.128
0.132
0.14
0.13
0.134
0.136
0.13
0.16
0.18
0.18
0.108
0.109
0.16
0.20
0.100
0.106
ERL-D
B. Blank Concentrations (ppb, recovery corrected)
Battelle 0.03 0.03 0.03 0.03
ERL-D --000
C. Surrogate Recoveries
Effluent
Battelle
77.8
86.0
100.0
103.3
77.8
74.6
100.0
101.1
84.4
80.0
68.0
68.4
123.9
119.6
64.4
86.7
100.0
90.2
ERL-D
Blank
Battelle 77.8 100.0 94.0 74.0
ERL-D -- 84.8 106.5 88.0
A-26
-------
Table A-20. In-Stream Concentration of Biphenyl for Stations 2 and 3 on Five Mile
Creek.
Concentration
in Discharge
Date
March 26
27
28
29
30
31
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
Average
Plant 1
^g/L
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
1.88
1.88
1.88
1.88
1.88
1.88
1.88
0.34
0.34
0.34
0.34
0.34
0.34
0.34
0.34
0.34
0.34
0.34
Plant 2
W/L
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.04
0.04
0.04
0.04
0.04
0.04
0.04
0.04
0.04
0.04
0.04
0.04
0.04
0.04
0.06
0.06
0.06
0.06
0.06
0.06
0.06
0.06
0.06
0.06
0.06
Standard Deviation
Coefficient of
Variation,
%157.
In-Stream Chemical
Concentration
Station 2
ng/L
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
15.83
12.49
10.79
11.17
16.54
21.68
19.62
3.46
2.59
0.99
0.00
0.00
1.82
3.07
2.85
2.06
2.19
2.74
4.06
6.39
67.0
Station 3
ng/L
0.00
0.00
0.00
0.00
0.00
0.00
0.00
7.31
6.75
7.31
7.66
7.11
5.62
6.30
19.61
18.31
15.47
16.15
19.99
23.51
22.20
15.76
15.12
13.60
13.02
13.17
13.89
14.85
16.33
14.48
16.00
15.46
10.8
7.22
A-27
-------
Table A-21. In-Stream Concentration of Phenanthrene for Stations 2 and 3 on Five
Mile Creek.
Concentration
in Discharge
Date
March
April
26
27
28
29
30
31
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
Average
Plant 1
H9/L
16.60
16.60
16.60
16.60
16.60
16.60
16.60
12.70
12.70
12.70
12.70
12.70
12.70
12.70
15.60
15.60
15.60
15.60
15.60
15.60
15.60
15.50
15.50
15.50
15.50
15.50
15.50
15.50
15.50
15.50
15.50
15.50
Plant 2
H9/L
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.03
0.03
0.03
0.03
0.03
0.03
0.03
0.07
0.07
0.07
0.07
0.07
0.07
0.07
0.20
0.20
0.20
0.20
0.20
0.20
0.20
0.20
0.20
0.20
0.20
Standard Deviation
Coefficient
of Variation,
% 44.0
In-Stream Chemical
Concentration
Station 2
ng/L
60.7
110.5
113.1
132.0
77.0
68.8
80.5
61.6
71.6
135.2
84.5
125.5
19.7
83.0
131.4
103.6
89.5
92.7
137.2
179.9
162.8
157.7
118.2
44.9
0.0
0.0
82.9
139.9
129.9
93.8
99.8
124.7
97.3
42.8
37.7
Station 3
ng/L
51.4
90.4
92.1
106.8
64.5
57.3
66.1
53.0
60.1
108.6
70.3
102.9
19.5
67.4
112.4
95.2
81.6
83.2
116.3
147.5
136.2
161.4
131.9
76.2
43.4
43.9
102.8
145.5
142.7
113.7
120.9
135.9
93.8
35.4
A-28
-------
Table A-22. In-Stream Concentration of Anthracene for Stations 2 and 3 on Five Mile
Creek.
Concentration
in Discharae
Date
March 26
27
28
29
30
31
April 1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
Average
Standard
Plant 1
Lig/L
7.26
7.26
7.26
7.26
7.26
7.26
7.26
4.45
4.45
4.45
4.45
4.45
4.45
4.45
6.68
6.68
6.68
6.68
6.68
6.68
6.68
7.22
7.22
7.22
7.22
7.22
7.22
7.22
7.22
7.22
7.22
7.22
Deviation
Coefficient of Variation,
Plant 2
H9/L
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
0.02
% 46.4
In-Stream Chemical
Concentration
Station 2
ng/L
26.5
48.3
49.5
57.7
33.7
30.1
35.2
21.6
25.1
47.4
29.6
44.0
6.9
29.1
56.2
44.4
38.3
39.7
58.8
77.0
69.7
73.5
55.0
20.9
0.0
0.0
38.6
65.2
60.5
43.7
46.5
58.1
41.6
19.3
41.0
Station 3
ng/L
24.1
41.1
42.0
48.7
30.0
26.7
30.9
20.3
22.7
39.8
26.4
37.7
8.2
25.1
46.2
38.6
33.2
33.7
47.9
61.3
56.5
59.1
45.4
19.8
4.3
4.4
32.6
52.4
49.1
37.2
38.7
46.9
35.3
14.5
A-29
-------
Table A-23. In-Stream Concentration of Fluoranthene for Stations 2 and 3 on
Five Mile Creek.
Concentration
in Discharge
Date
March 26
27
28
29
30
31
April 1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
Average
Standard
Plant 1
*ig/L
21.10
21.10
21.10
21.10
21.10
21.10
21.10
17.40
17.40
17.40
17.40
17.40
17.40
17.40
21.40
21.40
21.40
21.40
21.40
21.40
21.40
21.40
21.40
21.40
21.40
21.40
21.40
21.40
21.40
21.40
21.40
21.40
Deviation
Coefficient of Variation,
Plant 2
^L
0.19
0.19
0.19
0.19
0.19
0.19
0.19
0.23
0.23
0.23
0.23
0.23
0.23
0.23
0.23
0.23
0.23
0.23
0.23
0.23
0.23
0.28
0.28
0.28
0.28
0.28
0.28
0.28
0.28
0.28
0.28
0.28
% 44.8
In-Stream Chemical
Concentration
Station 2
ng/L
77.1
140.5
143.8
167.8
97.8
87.4
102.4
84.4
98.1
185.2
115.8
172.0
27.0
113.7
180.2
142.2
122.8
127.2
188.2
246.8
223.3
217.7
163.2
62.0
0.0
0.0
114.4
193.2
179.4
129.5
137.8
172.2
132.
59.0
31.6
Station 3
ng/L
89.0
137.8
142.9
165.6
108.0
97.6
113.3
107.2
114.2
183.3
132.4
174.5
53.3
122.1
179.9
159.6
135.9
140.2
184.9
226.6
211.4
223.6
183.0
106.0
60.8
61.5
142.7
201.7
197.9
157.9
167.8
188.5
146.
46.1
A-30
-------
Table A-24. In-Stream Concentration of Pyrene for Stations 2 and 3 on Five Mile
Creek.
Concentration
in Discharqe
Date
March 26
27
28
29
30
31
April 1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
Average
Standard
Plant 1
W/L
14.40
14.40
14.40
14.40
14.40
14.40
14.40
12.00
12.00
12.00
12.00
12.00
12.00
12.00
15.70
15.70
15.70
15.70
15.70
15.70
15.70
17.40
17.40
17.40
17.40
17.40
17.40
17.40
17.40
17.40
17.40
17.40
Deviation
Coefficient of Variation, %
Plant 2
H9/L
0.10
0.10
0.10
0.10
0.10
0.10
0.10
0.12
0.12
0.12
0.12
0.12
0.12
0.12
0.12
0.12
0.12
0.12
0.12
0.12
0.12
0.13
0.13
0.13
0.13
0.13
0.13
0.13
0.13
0.13
0.13
0.13
47.0
In-Stream Chemical
Concentration
Station 2
ng/L
52.6
95.9
98.1
114.5
66.8
59.7
69.9
58.2
67.7
127.7
79.9
118.6
18.6
78.4
132.2
104.3
90.1
93.3
138.1
181.0
163.9
177.0
132.7
50.4
0.0
0.0
93.1
157.1
145.8
105.3
112.0
140.0
97.6
45.8
36.5
Station 3
ng/L
56.5
89.9
92.8
107.6
69.0
62.1
72.1
66.8
72.3
119.4
83.9
113.5
31.3
78.2
122.6
106.5
91.0
93.4
126.4
157.4
146.1
160.4
127.4
65.2
28.2
28.5
95.6
143.5
137.7
107.3
113.0
131.4
96.8
35.4
A-31
-------
Table A-25. Tissue Target Analysis for Decapoda From Four Stations on Five Mile
Creek, Jefferson County, Alabama.
Upstream
Station 1
Tissue Concentrations (ppb,
Blphenvl
Batlelle
ERL-D
Phenanthrene
Batlelle
ERL-D
Anthracene
Batlelle
ERL-D
Fluoranthene
Battelle
ERL-D
Pyrene
Battelle
ERL-D
67.5
64.1
1.24
1.07
55.6
64.2
44.5
55.3
3.82
5.26
3.34
1.91
18.4
23.5
23.2
28.4
17.4
19.9
14.9
14.3
Discharge
Station 2
recovery corrected)
91.7(78.4)'
19.9
75.2
34.3 (6.81)
12.8
294(116)
247
70.8
79.5 (69.5)
260
78.5 (29.4)
144
31.7
23.8 (19.7)
25.2
440 (130)
537
88.2
121 (115)
112
393 (121)
491
88.2
132(91.3)
87.4
US31 Bridge
Station 3
41.9
57.3
3.13
140(116)
39.4
64.6
48.1
2720 (2720)
16.8
32.2
15.3
366 (290)
48.3
75.4
82.0
2060 (1700)
40.3
59.2
51.5
1010(812)
3m i Down
Station 4
67.0
69.4
3.54
2.40
57.6
50.6
38.2
21.1
25.2
19.9
11.0
6.47
89.7
68.0
69.4
36.6
75.3
59.8
51.0
26.6
A-32
-------
Table A-25. continued.
Upstream
Station 1
Discharge
Station 2
US31 Bridge
Station 3
3m i Down
Station 4
Surrogate Recoveries for Tissues
Biphenvl
Battelle
ERL-D
Phenanthrene
Battelle
ERL-D
Anthracene
Battelle
ERL-D
Fluoranthene
Battelle
ERL-D
Pyrene
Battelle
ERL-D
67.0
51.0
67.1
62.6
80.0
55.5
78.5
77.4
78.5
60.5
78.7
75.2
62.5
47.5
—
57.0
45.5
87.0
86.3
42.0 (44.0)
65.5
45.5
61.7(61.2)
53.8
39.0(41.0)
35.5
35.5
72.8 (74.5)
58.2
43.0 (44.0)
38.5
37.5
74.3 (74.2)
59.6
38.5(41.5)
36.0
39.5
—
40.0 (40.5)
36.0
40.5
84 (82)
89.1
51.0
56.5
69.9
37.0 (49.8)
41.0
33.0
65.1
50.7 (48.2)
42.5
31.0
64.1
62.0 (74.6)
47.0
37.5
—
49.0
39.0
88.4
61.7(71.5)
37.0
38.5
59.6
53.7
76
28.5
40.5
69.9
75.3
32.0
43.0
68.2
69.7
30.0
43.5
_„
32.0
43.5
82.4
83.4
8 Duplicate analysis.
A-33
-------
Table A-26. Tissue Target Analysis for Lepomis sp. From Four Stations on Five Mile
Creek, Jefferson County, Alabama.
Upstream
Station 1
Discharge
Station 2
US31 Bridge
Station 3
3m i Down
Station 4
Tissue Concentrations (ppb, recovery corrected)
Biphenyl
Battelle
ERL-D
Phenanthrene
Battelle
ERL-D
Anthracene
Battelle
ERL-D
Fluoranthene
B a tie lie
ERL-D
Pvrene
Battelle
ERL-D
63.0
61.0
4.93
7.76
95.0
97.0
47.0
69.0
10.4
14.2
5.19
3.53
18.8
24.2
14.2
19.6
11.9
17.4
9.52
7.82
56.9
65.2
12.5
3.14
5.81
55.6
99.8
81.7
34.3
53.9
30.3
28.5
15.5
5.76
8.84
29.1
29.8
40.5
12.4
24.1
12.4
14.9
10.1
5.47
8.31
74.7
69.9
7.29
11.9
48.4
70.9
39.5
72.6
20.1
21.6
10.2
20.3
19.0
35.8
24.5
51.7
12.9
15.2
8.85
16.2
68.7 (73.6)a
57.0
6.38 (6.08)
4.59
5.29
29.2 (35.2)
26.3
27.8 (26.5)
26.3
26.3
20.5 (24.4)
13.47
11.6(11.3)
9.59
10.1
18.5 (21.0)
16.3
27.9 (27.6)
22.2
25.7
8.51 (9.47)
7.67
52.8(10.5)
8.07
9.83
A-34
-------
Table A-26. continued.
Upstream
Station 1
Discharge
Station 2
US31 Bridge
Station 3
3m i Down
Station 4
Surrogate Recoveries for Tissues
Biphenyl
Battelle
ERL-D
Phenanthrene
Battelle
ERL-D
Anthracene
Battelle
ERL-D
Fluoranthene
Battelle
ERL-D
Pyrene
Battelle
ERL-D
51.0
59.0
50.1
68.3
59.5
60.0
50.9
76.2
51.5
45.5
49.9
73.7
53.0
55.5
—
52.5
56.5
68.7
79.5
42.5
40.0
67.0
46.2
53.7
43.5
42.5
79.3
58.0
60.7
31.0
40.0
76.1
55.6
59.3
50.5
47.0
_H
48.5
47.0
81.0
79.0
66.3
42.0
34.5
62.1
54.7
42.5
37.5
73.6
66.7
41.0
31.5
71.9
64.0
43.5
38.5
—
44.5
39.5
76.8
69.6
45.0 (47.0)
43.5
42.0 (40.8)
56.7
76.0
47.0(51.0)
41.5
43.6 (39.3)
52.8
86.2
37.0 (39.5)
35.5
43.5 (38.2)
53.6
84.9
47.0 (52.0)
42.5
—
47.0 (52.0)
42.0
58.5(51.0)
74.7
86.8
" Duplicate analysis.
A-35
-------
Table A-27. Blank Concentrations and Surrogate Recoveries for Tissue Analysis.
Blank Concentrations Surrogate
(ppb, recovery corrected) Recoveries
Biphenvl
Battelle 10.0 52.5
24.1 45.0
ERL-D 0 53.5
0.230 50.5
0.242 53.7
Phenanthrene
Battelle 2.10 52.0
3.00 52.0
ERL-D 1.28 61.4
1.51 47.7
1.50 48.0
Anthracene
Battelle 0.20 53.5
0.20 52.5
ERL-D 0 59.4
0 44.6
0 45.6
Fluoranthene
Battelle 0.80 56.0
0.90 56.5
ERL-D 0.297*
0
0
Pyrene
Battelle 0.45 54.0
0.65 57.5
ERL-D 0.747 75.8
0.565 75.0
0.666 67.6
' d,0-Pyrene used for recovery correction.
A-36
-------
Table A-28. Percent Lipid Content in Tissues From Four Stations on Five Mile Creek,
Jefferson County, Alabama.
Upstream Discharge US31 Bridge 3mi Down Background
ERL-D Station 1 Station 2 Station 3 Station 4
Decapoda
3.50
3.15
2.24 (2.50)
2.08
Lepomis sp. 3.99
4.58
Ictalarus
puctatus
2.24
4.16
Battelle (no site indicated)
Decapoda 2.0
Lepomis sp. 2.9
2.83
1.80(1.40)
3.00
0.893
Average (n = 8)
2.43
4.10
2.83
4.55
3.20
4.76
5.60(6.13)
4.22
4.22
1.95
2.60
Average (n = 10)
4.23
3.46
Average (n = 5)
2.88
A-37
-------
Table A-29. Tissue Target Analysis for Ictalarus puctatus From Caged Exposures at
Five Mile Creek, Jefferson County, Alabama.
Upstream
Station 1
Tissue Concentrations
Biphenyl
ERL-D
Phenanthrene
ERL-D
Anthracene
ERL-D
Fluoranthene
ERL-D
Pyrene
ERL-D
Surrogate Recoveries
Biphenyl
ERL-D
Phenanthrene
ERL-D
Anthracene
ERL-D
Fluoranthene
ERL-D
Pyrene
ERL-D
(ppb, recovery
15.2
17.4
106
112
12.4
11.5
33.5
32.6
16.9
16.0
for Tissues
65.1
56.3
72.7
113.8
73.6
117.3
«
77.4
79.5
US31 Bridge
Station 3
corrected)
3.36
4.32
13.6
13.0
6.73
7.50
17.4
14.9
11.0
8.43
67.0
61.6
79.3
66.0
76.1
63.7
--
81.0
85.3
Background
1.44
7.25
0.00
1.01
1.26
65.9
79.4
77.9
—
85.0
A-38
-------
Table A-30. Ambient Water Samples for Target Chemical Analysis for Five Mile
Creek, Birmingham, Alabama.
Ambient Station: Blank 123 4 56
A. Ambient Concentrations (ng/L, recovery corrected)
Biphenyl 0.460 6.52 2.11 1.95 710. 3.37 4.32
Phenanthrene 3.35 19.5 7.90 6.63 886. 12.7 43.5
Anthracene 2.72 6.91 3.96 3.35 188. 4.14 25.9
Fluoranthene 3.62 15.0 11.6 7.21 327. 10.4 167.
Pyrene 3.12 14.0 11.6 6.89 166. 10.4 154.
B. Surrogate Recoveries (%)
Biphenyl 89.1 62.1 84.5 84.7 69.3 74.7 22.7
Phenanthrene 90.7 62.6 86.5 87.5 88.2 76.4 25.6
Anthracene 86.4 59.6 84.2 80.8 92.7 74.6 25.7
Fluoranthene8 .... . .
Pyrene 89.9 70.2 97.9 91.1 97.3 79.5 29.6
d,0-Pyrene recoveries used for recovery correction.
A-39
-------
FIELD EVALUATION OF RESIDUE PREDICTION PROCEDURES
USED IN ERA'S GUIDANCE:
•ASSESSMENT AND CONTROL OF BIOCONCENTRATABLE CONTAMINANTS
IN SURFACE WATERS":
THE LOUISIANA STUDY
1993 DRAFT FOR APPENDIX I
Lawrence P. Burkhard1
Barbara Riedel Sheedy2
Nelson A. Thomas1
1U.S. Environmental Protection Agency
Environmental Research Laboratory-Duluth
6201 Congdon Boulevard
Duluth, MN 55804
2AScl Corporation
6201 Congdon Boulevard
Duluth, MN 55804
-------
Foreword
Recent advances in environmental sciences, analytical chemistry, and
toxicology have permitted the development of a systematic and scientifically
defensible procedure for identifying, assessing, and controlling chemicals which
form residues in fish and/or shellfish. This guidance procedure, "Assessment and
Control of Bioconcentratable Contaminants in Surface Waters", is applicable to
nonpolar organic chemicals which bioconcentrate and/or bioaccumulate in aquatic
organisms.
. The principal components of this newly developed guidance approach are:
a) analytical procedures for detecting and identifying bioconcentratable chemicals
in effluents or receiving water organisms, b) procedures to predict residues of
bioconcentratable chemicals in aquatic organisms, c) procedures for deriving
criteria for aquatic organisms and receiving waters for bioconcentratable chemicals,
and d) permitting guidance for control of these pollutants from point sources. The
guidance approach combines these procedures to arrive at discharge
concentrations for bioconcentratable chemicals which limit residues in aquatic
organisms used for human consumption.
This report presents results of a field study conducted on an estuarine bayou
with one point source discharge. The objective of the study was to determine how
well tissue residue concentrations can be predicted in field discharge situations
using the guidance residue prediction procedure. Thirteen chemicals were studied.
Disclaimer
This document has been reviewed in accordance with U.S. Environmental
Protection Agency policy and approved for publication. Mention of trade names or
commercial products does not constitute endorsement or recommendation for use.
-------
Acknowledgements
Technical assistance was provided by Donald Mount, Correne Jenson, Lara
Andersen, Christine Harper (AScI Corporation, Duluth, MN), Mark Yancey, Laura
Hernon-Kenny, Terry Nobles, Steve Summer, William Clement, Dennis Mclntyre
(Battelle Laboratories-Columbus Division), Mick DeGraeve, John Shuey, Dennis
McCauley, Robin Silva-Wilkinson (Battelle Laboratories-Great Lakes Division),
William Morrow and Robert Wood, U.S. EPA (Office of Wastewater Enforcement
and Compliance), and Kirk Manual and Larry Racca (State of Louisiana). The
clerical support of Debra Williams and Jane Norlander (AScI Corporation, Duluth,
MN) is gratefully acknowledged.
Financial support for this study was provided by the Office of Wastewater
Enforcement and Compliance.
-------
Contents
Page No.
Foreword 2
Disclaimer 2
Acknowledgements 3
Contents 4
Figures 6
Tables 7
Introduction 13
1.1 Site Study Objective 13
1,2 Constraints 14
Site .Selection and Description 15
2.1 Description of Bayou d'lnde, Calcasieu Parish, Louisiana 15
2.2 Screening of the Effluents 17
2.3 Selection of Target Chemicals 17
Methods 20
3.1 Site Study Plan 20
3.2 Estimation of Residues in Aquatic Organisms 20
3.2.1 Prediction of Bioconcentration Factors
for Aquatic Organisms 20
3.2.2 Prediction of Bioaccumulation Factors
for Aquatic Organisms 21
3.2.3 Prediction of Residues in Aquatic Organisms 21
3.2.4 Metabolism and Prediction of Residues in Aquatic
Organisms 21
3.3 Sampling Procedures 22
3.3.1 Field Sampling Procedures for the Composite
Ambient Water Samples 22
3.3.2 Field Sampling Procedures for the Grab Ambient
Water Samples 22
3.3.3 Field Sampling Procedure for the Grab Water
Samples from the Outfall 22
3.3.4 Field Procedures for Sampling Sediments 23
3.3.5 Field Sampling Procedures for Indigenous Organisms 23
-------
3,4 Analytical Procedures 23
3.4.1 Effluent Analysis Procedure 23
3.4.2 Weekly Ambient Water (Grab and Composite) and
Outfall (Grab) Samples Analysis Procedure 25
3.4.3 Tissue Analysis Procedure 27
3.4.3.1 Compositing and Homogenization
Procedures 27
3.4.3.2 Tissue Analysis 28
3.4.4 Sediment Analysis Procedure 30
3.4.5 Dissolved and Paniculate Organic Carbon
Analysis Procedures 31
3.4.6 Procedures for Particle Sizing the Sediments 31
Results and Discussion 32
4.1 Expected Tissue Residues Trends 32
4.2 Ambient Water Concentrations: Results 32
4.3 Ambient Water Concentrations: Discussion 36
4.4 Sediment Results 37
4.5 Tissue Data: Results 42
4.6 Prediction of the Tissue Residues 42
4.7 Comparison of the Predicted and Observed Tissue
Residues: Results 61
4.8 Comparison of the Predicted and Observed Tissue
Residues: Discussion 63
4.9 Summary 68
References 69
Appendix A: Study Data 72
-------
Figures
Page No.
Figure
2-1 Location of sampling sites during a toxicity-characterization study 16
of the lower Calcasieu River and Bayou d'lnde, Louisiana, June 1988,
from Demcheck et al. 1990 [5].
-------
Tables
Page No.
Table
2-1 Site Study Chemicals. 18
3-1 Common and Scientific Names of the Organisms Collected as Six
Sample Stations Near Lake Charles, Louisiana. 24
4-1 Concentration of Target Chemicals in Weekly Ambient Water Samples
from the Canal and Bayou d'lnde. 33
4-2 Concentration of Target Chemical in Sediment Samples from the
Canal and Bayou d'lnde. 38
4-3 Concentration of Target Chemicals in Fundulus heteroclitus from
• Cooling Water/Effluent Canal and Bayou d'lnde. 43
4-4 Concentration of Target Chemicals in Callinectes sapidus from
Cooling Water/Effluent Canal and Bayou d'lnde. 44
4-5 Concentration of Target Chemicals in Brevoortia patronus from
Cooling Water/Effluent Canal and Bayou d'lnde. 45
4-6 Concentration of Target Chemicals in Microoogan undulus from
Cooling Water/Effluent Canal and Bayou d'lnde. 46
4-7 Residue Prediction Parameters. 47
4-8 Predicted and Measured Fundulus heteroclitus Tissue Concentrations
for the Louisiana Study. 48
4-9. Predicted and Measured Callinectes sapidus Tissue Concentrations
for the Louisiana Study. 52
4-10 Predicted and Measured Brevoortia patronus Tissue Concentrations
for the Louisiana Study. 56
4-11 Predicted and Measured Micropogan undulus Tissue Concentrations
for the Louisiana Study. 58
4-12 Distribution of the Ratios of the Observed to Predicted Tissue
Concentrations for All Field Stations. 62
-------
Tables{continued!
4-13 Distribution of the Ratios of the Observed to Predicted Tissue
Concentrations for Field Stations A, 1-G, and B 66
4-14 Distribution of the Ratios of the Observed to Predicted Tissue
Concentrations for Field Stations A, 1-G, B, D, and E. 67
8
-------
Appendix A Tables
Table A-1. Bioconcentratable Chemicals Tentatively Identified Using the Effluent
Analytical Procedure: Outfall, Fraction 1.
Table A-2. Bioconcentratable Chemicals Tentatively Identified Using the Effluent
Analytical Procedure: Outfall, Fraction 2.
Table A-3. Bioconcentratable Chemicals Tentatively Identified Using the Effluent
Analytical Procedure: Outfall, Fraction 3.
Table A-4. Bioconcentratable Chemicals Tentatively Identified Using the Effluent
Analytical Procedure: Station 1, Fraction 1.
Table A-5. Bioconcentratable Chemicals Tentatively Identified Using the Effluent
Analytical Procedure: Station 1, Fraction 2.
Table A-6. Bioconcentratable Chemicals Tentatively Identified Using the Effluent
Analytical Procedure: Station 1, Fraction 3.
Table A-7. Concentrations of Hexachloroethane in Ambient Water Samples.
Table A-8. Concentrations of Tetrachlorobutadiene #1 in Ambient Water
Samples.
Table A-9. Concentrations of Tetrachlorobutadiene #2 in Ambient Water
Samples.
Table A-10. Concentrations of Pentachlorobutadiene #1 in Ambient Water
Samples.
Table A-11. Concentrations of Pentachlorobutadiene #2 in Ambient Water
Samples.
Table A-12. Concentrations of Hexachlorobutadiene in Ambient Water Samples.
Table A-13. Concentrations of 1,2,3-Trichlorobenzene in Ambient Water Samples.
Table A-14. Concentrations of 1,2,4-Trichlorobenzene in Ambient Water Samples.
Table A-15. Concentrations of 1,2,4,5- and 1,2,3,5-Tetrachlorobenzene in
Ambient Water Samples.
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Appendix A Tables (continued)
Table A-16. Concentrations of 1,2,3,4-Tetrachlorobenzene in Ambient Water
Samples.
Table A-17. Concentrations of Pentachlorobenzene in Ambient Water Samples.
Table A-18. Concentrations of Hexachlorobenzene in Ambient Water Samples.
Table A-19. Dissolved Chemical Concentrations for Ambient Composite Water
Samples from Station 1.
Table A-20. Particulate Chemical Concentrations for Ambient Composite Water
Samples from Station 1.
Table A-21. POC and DOC for Ambient Water Samples.
Table A-22. DOC, POC, and TOC Values for Ambient Water Samples.
Table A-23. Percentages of Daily Composite Versus Daily Grab Samples in the
Seven Day Composite Water Samples from Station 1.
Tablg A-24. Organic Carbon Content for Sediment Samples.
Table A-25. Particle Size Distribution for Sediments.
Table A-26. Tissue Compositing Information
Table A-27. Hexachloroethane Tissue Concentrations for Fundulus heteroclitus.
Table A-28. Tetrachlorobutadiene #1 Tissue Concentrations for Fundulus
heteroclitus.
Table A-29. Tetrachlorobutadiene #2 Tissue Concentrations for Fundulus
heteroclitus.
Table A-30. Pentachlorobutadiene #1 Tissue Concentrations for Fundulus
heteroclitus.
Table A-31. Pentachlorobutadiene #2 Tissue Concentrations for Fundulus
heteroclitus.
Table A-32. Hexachlorobutadiene Tissue Concentrations for Fundulus heteroclitus.
10
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Appendix A Tables (continued)
Table A-33. 1,2,3-Trichlorobenzene Tissue Concentrations for Fundulus
heteroclitus.
Table A-34. 1,2,4-Trichlorobenzene Tissue Concentrations for Fundulus
heteroclitus.
Table A-35. 1,2,4,5- and 1,2,3,5- Tetrachlorobenzene Tissue Concentrations for
Fundulus heteroclitus.
Table A-36. 1,2,3,4- Tetrachlorobenzene Tissue Concentrations for Fundulus
heteroclitus.
Table A-37. Pentachlorobenzene Tissue Concentrations for Fundulus heteroclitus.
Table A-38. Hexachlorobenzene Tissue Concentrations for Fundulus heteroclitus.
Table A-39. Hexachloroethane Tissue Concentrations for Callinectes sapidus.
Table A-40. Tetrachlorobutadiene #1 Tissue Concentrations for Callinectes
sapidus.
Table A-41. Tetrachlorobutadiene #2 Tissue Concentrations for Callinectes
sapidus.
Table A-42. Pentachlorobutadiene #1 Tissue Concentrations for Callinectes
sapidus.
Table A-43. Pentachlorobutadiene #2 Tissue Concentrations for Callinectes
sapidus.
Table A-44. Hexachlorobutadiene Tissue Concentrations for Callinectes sapidus.
Table A-45. 1,2,3-Trichlorobenzene Tissue Concentrations for Callinectes sapidus.
Table A-46. 1,2,4-Trichlorobenzene Tissue Concentrations for Callinectes sapidus.
Table A-47. 1,2,4,5- and 1,2,3,5-Tetrachlorobenzene Tissue Concentrations for
Callinectes sapidus.
Table A-48. 1,2,3,4-Tetrachlorobenzene Tissue Concentrations for Callinectes
sapidus.
Table A-49. Pentachlorobenzene Tissue Concentrations for Callinectes sapidus.
11
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Appendix A Tables (continued)
Table A-50. Hexachlorobenzene Tissue Concentrations for Callinectes sapidus.
Table A-51. Hexachloroethane Tissue Concentrations for Brevoortia patronus and
Micropogan undulus.
Table A-52. Tetrachlorobutadiene #1 Tissue Concentrations for Brevoortia
patron us and Micropoaan undulus.
Table A-53. Tetrachlorobutadiene #2 Tissue Concentrations for Brevoortia
patronus and Micropogan undulus.
Table A-54. Pentachlorobutadiene #1 Tissue Concentrations for Brevoortia
patronus and Micropoaan undulus.
Table A-55. Pentachlorobutadiene #2 Tissue Concentrations for Brevoortia
patronus and Micropogan undulus.
Table A-56. Hexachlorobutadiene Tissue Concentrations for Brevoortia patronus
and Micropogan undulus.
Table A-57. 1,2,3-Trichlorobenzene Tissue Concentrations for Brevoortia patronus
and Micropogan undulus.
Table A-58. 1,2,4-Trichlorobenzene Tissue Concentrations for Brevoortia patronus
and Micropogan undulus.
Table A-59. 1,2,4,5- and 1,2,3-5 Tetrachlorobenzene Tissue Concentrations for
Brevoortia patronus and Micropogan undulus.
Table A-60. 1,2,3,4-Tetrachlorobenzene Tissue Concentrations for Brevoortia
patronus and Micropogan undulus.
Table A-61. Pentachlorobenzene Tissue Concentrations for Brevoortia patronus
and Micropogan undulus.
Table A-62. Hexachlorobenzene Tissue Concentrations for Brevoortia patronus and
Micropogan undulus.
12
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INTRODUCTION
The Environmental Protection Agency has developed a guidance procedure,
"Assessment and Control of Bioconcentratable Chemicals in Surface Waters" [1],
to control bioconcentratable chemicals in effluents. This guidance consists of a
number of technical procedures that have been developed during the past several
years. The principal components of the guidance approach are: 1) analytical
procedures for detecting and identifying bioconcentratable chemicals in effluents,
receiving water, and organisms, 2) prediction of the bioconcentration factor (BCF)
from the n-octanol water partition coefficient (P) using quantitative structure
activity relationships (QSAR), 3} prediction of the bioaccumulation factor (BAF)
from the chemical's BCF and log P, and the trophic status of the organism of
concern, 4) prediction of residues in aquatic organisms using the BCF or BAF and
concentration of the chemical in the receiving water, and 5} calculation of
allowable ambient water or tissue residue concentrations for bioconcentratable
chemicals based upon human consumption of contaminated fish and shellfish. The
guidance protocol combines these procedures to arrive at discharge concentrations
for bioconcentratable chemicals which will limit residues in aquatic organisms used
for human consumption.
The guidance approach provides two alternatives for assessing point source
discharges for bioconcentratable chemicals, the effluent and tissue alternatives
(component 1). With these alternatives, either effluent from a point source
discharge or indigenous receiving water organisms are analyzed. Results from the
analytical methods for both alternatives are listings of bioconcentratable chemicals.
These results are evaluated further using components 2 through 5, to determine if
development of permit limits are needed for any of the identified bioconcentratable
chemicals.
With the tissue alternative, the analytical results provide information for the
entire receiving water since the aquatic organisms provide an integrated
assessment of all point and nonpoint sources of bioconcentratable chemicals.
When an unallowable tissue residue is found, additional chemical analyses are
required to determine the source(s) of the residue forming chemical to the receiving
water. In contrast, with the effluent alternative, point source discharges are
examined individually. The inclusion of both alternatives in the guidance provides
greater flexibility and usefulness for the guidance approach since neither alternative
by itself is useful in all permitting situations.
1.1 Site Study Objective
" The objective of the site study was to determine how well tissue residue
concentrations can be predicted in field discharge situations using the guidance
procedures, i.e., components 2, 3, and 4.
13
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This validation effort was not designed to verify a) the accuracy of the
allowable tissue residues, b) the analytical procedures associated with the tissue
alternative, c) the prediction of residues where exposure is intermittent, d) the
prediction of residues where exposure is difficult to estimate, or e) the derivation
of acceptable human uptake levels.
1.2 Constraints
In order to predict residues in receiving water organisms, the concentration
of th'e chemicals in the receiving water must be known, and these concentrations
(in the receiving water) must be relatively constant for a 20 to 40 day period.
Without these conditions, successful evaluation of the field data will be nearly
impossible since the indigenous organisms will never come to steady-state
conditions with the receiving water.
These characteristics, in general, are associated with sites which: a) have
reasonably simple hydrodynamics so that receiving water concentrations can be
determined and/or calculated, b) have short hydraulic resident times so that fate
and halflife considerations are minimized for the discharged chemicals, c) have
effluent discharges with relatively constant concentrations of bioconcentratable
chemicals, and d) have limited sources of the bioconcentratable chemicals under
investigation.
14
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SITE SELECTION AND DESCRIPTION
This report details the validation study performed on Bayou d'lnde, Calcasieu
Parish, Louisiana in September and October, 1990. This field site was selected
because a) the effluent upon assessment with the effluent alternative analytical
method contained bioconcentratable chemicals, b) the flow regime of the site was
reasonably simple and had short flow times, and c) native populations of fish and
shellfish were available. Furthermore, preliminary calculations suggested that
concentrations of the chemicals in the receiving water were high enough to result
in measurable tissue residues in the indigenous organisms.
2.1 Description of Bayou d'lnde, Calcasieu Parish, Louisiana
The site selected for the validation study was a 1.6 km stretch of Bayou
d'lnde and a 1.6 km length of an effluent/cooling water canal which flows in to the
bayou (Figure 2-1). This site is located about 6.4 km west of Lake Charles,
Louisiana and is in the lower Calcasieu River system (Figure 2-1). The
effluent/cooling water canal receives a discharge from a chemical manufacturing
plant which produces a variety of synthetic organic chemicals. The
effluent/cooling water canal is a dredged channel, 3 m deep and 20 m wide. Three
bridges cross the canal within the study area, and these bridges provide access to
natural gas and oil wells in the marshes adjacent to the canal.
Bayou d'lnde is a lowland channel that meanders through a tidally-influenced
brackish/freshwater marsh. Water from Bayou d'lnde flows in a southeasterly
direction into the Calcasieu River ship canal, and Bayou d'lnde is used for
commercial navigation. Commercial navigation in the bayou consists primarily of
petroleum carrying barges and barge tows. Water levels in Bayou d'lnde and the
surrounding marsh fluctuate with the daily tidal cycle, and with seiches generated
by northerly and southerly winds on the lower Calcasieu River system. The water
level of the effluent canal is influenced by the fluctuating water levels in Bayou
d'lnde. Typical tidal fluctuations are about 15 cm in the bayou [2].
Bayou d'lnde is a highly industrialized estuary and the bayou receives
discharges from at least five different manufacturing facilities. The bayou is highly
contaminated with metals and chlorinated nonpolar organics [3,4]. In general, the
water quality of the bayou is highly degraded, and numerous EPA criteria and state
water quality standards are exceeded.
15
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Figure 2-1. Location of sampling sites during a toxicity-characterization study of the tower Calcasieu River and Bayou
d'lnde, Louisiana, June 1988, from Demcheck et al. 1990 [5j.
Industrial
Outfall
Canal
EXPLANATION
V Sampling site
16
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After discovery of the contaminant problem in 1986 and 1987, advisories
were issued by the Louisiana Department of Environmental Quality and the
Louisiana Department of Health and Hospitals against the consumption of fish and
shellfish and against swimming, wading, and water sports in Bayou d'lnde. In
1989, advisories against the sale and consumption of speckled trout and white
trout from the lower Calcasieu estuary were issued in light of the high fish and
shellfish tissue residue levels of hexachlorobenzene and hexachlorobutadiene.
Seven sample collection sites were used for this study (Figure 2-1). This
figure was taken from a previous study [5], and therefore, the sample locations 1,
2, 3, 9, 10, and 11 correspond with stations A, 1, B, C, D, E, respectively, in this
study. For the effluent/cooling water canal, three stations, Stations A, 1, and B,
were located at the three bridges crossing the canal. Station A was furthest
upstream and Station 1 was 400 m downstream of Station A. Station B was 400
m downstream of Station 1, and approximately 400 m upstream of the confluence
of the effluent/cooling water canal and Bayou d'lnde.
Three stations, Stations C, D and E, were located in a navigable stretch of
Bayou d'lnde. Station C was located 400 m upstream of the confluence of the
effluent/cooling water canal and Bayou d'lnde. Stations D and E were located 400
and 800 m, respectively, downstream of the confluence of the effluent/cooling
water canal and Bayou d'lnde.
The seventh sampling station was the outfall from the chemical plant prior
to dilution with cooling water. The outfall after dilution with cooling water enters
the upstream end of the canal. The canal is composed of 100% effluent, I.e.,
outfall after dilution with cooling water, and Station 1 corresponds to the NPDES
permitted sample location for the chemical manufacturing facility.
2.2 Screening of the Effluents
Prior to the site study, the effluent analytical method was performed on grab
effluent samples from Station 1 and the outfall. This method detected and
identified a number of chlorinated organics, i.e., chloro-benzenes and chloro-
butadienes, and a few polycyclic aromatic hydrocarbons (PAHs). Data for these
analyses are reported in Appendix A (Tables A-1 through A-6) for the samples from
Station 1 and the outfall.
2.3 Selection of Target Chemicals
For this study, thirteen chemicals were chosen for evaluation. In Table 2-1,
a listing of the thirteen site study chemicals, their abbreviations, and BCFs are
reported. Six of the thirteen chemicals were identified by using the effluent
analytical procedure on the outfall and Station 1 grab samples prior to the study
(Table 2-1). Of the remaining seven chemicals, two were isomers and five were
structural analogs of the chemicals identified using the effluent analytical
procedure.
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Table 2.1 Site Study Chemicals
Full Chemical Name
Hexachloroethane
Tetrachlorobutadiene #1
Tetrachlorobutadiene #2
Pentachlorobutadiene #1
Pentachlorobutadiene #2
Hexachlorobuta-1 ,3-diene
1 ,2,3-Trichlorobenzene
1 ,2,4-Trichlorobenzene
1,2. 4,5- and 1,2,3,5-
"Tetrachlorobenzene
1 ,2,3,4-Tetrachlorobenzene
Pentachlorobenzene
Hexachlorobenzene
Abbreviation
(HCE)
(TeCBD#1)
(TeCBD#2)
(PeCBD#1)
(PeCBD#2)
(HCBD)
(1,2,3-TrCB)
(1,2,4-TrCB)
(TeCB Mix)
(1,2,3,4-TeCB)
(PeCB)
(HCB)
Calculated
BCF
742
140
140
340
340
2380
630
597
2800
1840
4850
6240
Identified in
the Effluent
Analysis?
yes
no
no
yes
no
yes
no
yes
no
no
yes
yes
18
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All thirteen site study chemicals were present in the outfall and/or Station 1
grab samples even though they were not all identified with the effluent analytical
procedure. The inclusion of the seven remaining chemicals allowed a more
complete and comprehensive evaluation of the chlorinated butadiene and benzene
clashes of compounds.
Two of the selected chemicals, 1,2,4,5- and 1,2,3,5-TeCBs, could not be
consistently resolved in the instrumental analysis and therefore, these two
chemicals were treated as one in this site study. For the TeCBDs and PeCBDs, the
lack of reference materials, i.e., neat material, prevented direct confirmation of
each chemical. Therefore, the TeCBDs and PeCBDs were referred to as TeCBD#1,
TeCBD#2, PeCBD#1. and PeCBD#2 in this study.
' The chemicals selected for the site study were typical of the chemicals from
the discharge. Their calculated BCFs ranged from 140 to 6,420 (Table 2-1).
Some of the chemicals were available in stable isotope form; with stable isotopes,
recoveries for the chemicals through the analytical procedure can be determined for
each sample.
19
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METHODS
3.1 Site Study Plan
Measured residue levels in indigenous organisms for the Bayou d'lnde site
were compared to residue concentrations predicted for these organisms.
Residue levels in the organisms were predicted by determining the ambient
chemical concentrations and using these data in the residue prediction procedure.
Ambient water concentrations were determined by collecting and analyzing one
grab'water sample every seven days for four weeks at six sampling stations.
Three of the sampling stations were located in the canal, and the remaining three
stations were located in the bayou. One of the bayou stations was above the
confluence of the canal and the bayou. In conjunction with the collection of the
grab samples, a series of four, seven-day 24-hour composite ambient water
samples were collected and analyzed from one of the sampling stations located on
the canal.
Indigenous organisms were collected at the end of the 28 day period at the
six stations used for the collection of the ambient water samples. Residue
analyses and lipid content determinations were performed on the indigenous
organisms.
Replicate chemical analyses were performed on the ambient water samples
and indigenous organisms by two analytical laboratories. These analyses included
both inter- and intra-laboratory replication of the ambient water samples. For the
tissue samples, replicate analyses were performed on selected samples when
enough tissue mass was available.
3.2 - Estimation of Residues in Aquatic Organisms
Only a brief description of the residue prediction technique is presented here.
The reader is referred to EPA 1991 [1] for further details.
3.2.1 Prediction of Bioconcentration Factors for Aquatic Organisms
Bioconcentration factors for aquatic organisms were estimated using the
multi-species log BCF-log P correlation developed by Veith and Kosian [6]:
log BCF = 0.79 log P - 0.40 n = 112 r2 = 0.86
This correlation, derived from a data set consisting of 122 BCF values for 13
freshwater and marine species, was typical of all log BCF-log P correlations [7].
The above equation has 95% prediction intervals (note, 95% confidence intervals
for the mean BCF are much smaller) of approximately one order of magnitude, and
the predicted BCF values were for organisms with 7.6% lipid content.
20
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The predicted BCF values must be corrected to the appropriate lipid content
before prediction of the tissue residues since numerous fishes and shellfishes have
lipid contents differing from 7.6%. The BCF is directly proportional to lipid
content, and corrections for lipid content were done using a simple proportionality.
3.2.2 Prediction of Bioaccumulation Factors for Aquatic Organisms
Bioaccumulation factors are derived by "adjusting" the BCF using a food
chain multiplier (FM) for the organism of concern [1]:
BAF = FM * BCF
The FM is dependent upon the log P of the chemical and the structure of the
organism's food chain [8-10].
In this site study, the FMs for the.chemicals under investigation were equal
to 1.O for eleven of the thirteen chemicals due to their relatively low log P values
and consequently, the BAF and BCF were equal for these chemicals. For two other
chemicals, PeCB and HCB, their FMs were 3.0 and 3.7, respectively. For different
chemicals, readers should consult EPA 1991 [1] to obtain the appropriate FM
value.
3.2.3 Prediction of Residues in Aquatic Organisms
The tissue residues for a chemical were calculated by multiplying the BAF,
the product of the BCF and FM terms, after correction for lipid content, by the
concentration of the chemical in the water:
[Fish] = BAF * [Water] = BCF * FM * [Water]
The [Fish] and [Water] terms are the concentration of the chemical in the aquatic
organism and in the receiving water, respectively. Residue concentrations
predicted using the BCF or BAF were for steady-state conditions which implies that
the concentration of a chemical in the receiving water was at steady-state also.
3.2.4 Metabolism and Prediction of Residues in Aquatic Organisms
The tissue residues predicted using the procedure outlined in Sections 3.2.1
through 3.2.3 assumes that in vivo metabolism of the contaminants does not
occur. When metabolism occurs, the predicted residues will be larger than those
measured in the organisms, because metabolism enhances excretion of zenobiotic
chemicals.
The effects of metabolism on the actual versus predicted tissue residue
concentrations is dependent upon the rate of metabolism for each chemical. For
chemicals with slow rates of metabolism, the differences between the predicted
and measured tissue residues will be small, while for chemicals with rapid rates of
metabolism, differences between the predicted and measured tissue residues will
be large.
21
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3.3 Sampling Procedures
3.3.1 Field Sampling Procedures for the Composite Ambient Water Samples
A series of four, seven-day, 24-hour composite ambient water samples were
collected from Station 1. Sample collection was initiated on September 12, 1990,
and was completed on October 10, 1990. The individual composites were taken
during the time periods of September 12-19, September 19-26, September 26 to
October 3, and October 3-10.
The samples were collected in an iced ISCO* water sampler equipped with a
glass collection vessel. The ISCO" sampler was inspected daily, at which time the
ice was replenished and the water samples were removed and placed in
refrigerated storage. Because of the heat and humidity at Station 1 during the
course of the study, the ISCO* sampler occasionally failed. On the days when a
24-hour composite sample was not available, or when the volume of sample was
insufficient, a grab sample from the canal was collected to supplement the daily
composite sample. At the end of each seven-day period, the individual 24-hour
samples were composited and mixed in a 60 liter carboy container. Replicate four
liter samples were drawn from the seven-day composite samples, placed into new
brown glass 1 gallon solvent bottles with teflon lined caps, packed on ice, and
shipped using overnight delivery to two analytical laboratories, Battelle-Columbus
and Environmental Research Laboratory-Duluth (ERL-D).
3.3.2 Field Sampling Procedures for the Grab Ambient Water Samples
On the seventh day of the collection of the seven-day composite ambient
water samples at Station 1, replicate four liter ambient canal and Bayou d'lnde
water samples were collected from stations A, 1, B, C, D and E. These samples
were collected on September 19, September 26, October 3, and October 10. The
only exception was the collection of the Station 1 grab sample which was taken on
the 20th instead of the 19th of September.
The ambient grab samples were collected at mid-channel/mid-water column
at each station using a battery operated electric pump. Upon collection, these
samples were placed into new brown glass 1 gallon solvent bottles with teflon
lined caps, packed on ice, and shipped using overnight delivery to Battelle and
EPA-Duluth.
3.3.3 Field Sampling Procedure for the Grab Water Samples From the Outfall.
. Replicate four liter grab samples were collected from the outfall from the
chemical plant prior to dilution with cooling water on September 20, September 26
October 3, and October 10, 1990. These samples were placed into new brown
glass 1 gallon solvent bottles with teflon lined caps, packed on ice, and shipped
using overnight delivery to ERL-D,
22
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3.3.4 Field Procedures for Sampling Sediments
Concurrently with the collection of the last ambient water grab samples (10
and 11 October 1990), sediment samples were collected from all six ambient
stations using an Eckman dredge sampler. Three samples were collected per
station, the quarter, mid-, and 3/4 quarters channel locations. Samples were
transferred to glass containers, capped with teflon lined lids, packed on ice, and
shipped to Battelle-Columbus and EPA-Duluth.
3.3.6 Field Sampling Procedures for Indigenous Organisms
Resident organisms were collected on October 8-11, 1990, using a
combination of variable mesh gill nets, cast nets, minnow traps and crab pots,
The common and scientific names of the organisms collected are listed in Table 3-
1. Of the ten species collected, two species, Fundulus heteroclitus and Callinectes
sapidus. were collected at all six stations. The third most common species,
Brevoortia patronus. was collected at five of the six stations. The fourth most
abundant species, Micropogan undulus. was collected at four of the six stations.
The six remaining species collected were present only at low numbers (one or two)
at few of the stations.
Immediately after collection, the organisms were placed in coolers containing
wet ice until they were labeled and frozen «24 h). Special care was taken to
insure that separate coolers were used for holding the organisms at each station.
The collected organisms were frozen whole in solvent rinsed aluminum foil
packages containing 3 to 10 organisms. Individual organisms that weighed more
than 100 grams were packaged separately. When the collection of resident
organisms was completed, the frozen packages of organisms were inventoried,
shipped on dry ice to Battelle-Great Lakes, and then shipped on dry ice to Battelle-
Columbus and ERL-D for compositing and analysis.
3.4 Analytical Procedures
3.4.1 Effluent Analysis Procedure
Only a brief account of the procedure for detecting and identifying
bioconcentratable chemicals in effluents will be presented here. Readers are
referred to Appendix B of EPA's guidance [1] for further details.
A 10 L effluent sample was spiked with three surrogate compounds, d10-
biphenyl, 13Ce-1,2,4,5-TeCB, and 13Ce-HCB, and extracted with hexane. The
hexane extract was subsequently cleaned up using sulfuric acid, and concentrated
to a volume of 0.50 ml. The extract was chromatographed using reverse phase
high pressure liquid chromatography (HPLC), and three fractions were collected.
The fractions were extracted, concentrated to 0.10 ml, and spiked with the
internal standard, d,2-chrysene. The fraction extracts were analyzed using capillary
gas chromatography with full scan electron impact ionization mass spectrometry
(GC/MS).
23
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Table 3-1. Common and Scientific Names of Organisms Collected at Six
Sample Stations Near Lake Charles, Louisiana.
Common Name Scientific Name"
Cockahoo (mummy chog)b Fundulus heteroclitus
Striped Mullet Mugil ceohalus
Blue Crabc Callinectes sapidus
Gulf Menhaden" Brevoortia patronus
Butterfish (spot) Leiostomus xanthurus
Atlantic Croaker* Micropogan undulus
Sea Catfish (hardhead) Arius filus
Marsh Killifish Fundulus confluentus
Banana Fish (Lady Fish) Elopes saurus
Fiddler Crab Uca puqilator
Scientific names of fishes taken from: Common and Scientific Names of
North American Fishes. American Fisheries Society. 1970.
Most common fish.
Most common invertebrate.
Third most common organism collected.
Fourth most common organisms collected.
24
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" Each chromatographic peak in the GC/MS chromatograms was quantified
using the response factor calculated from its appropriate surrogate. For fractions
one, two, and three, the quantification surrogates were d10-biphenyl, 13C6-1,2,4,5-
TeCB, and 13C6-HCB, respectively.
For each fraction, all chromatographic peaks were reverse-searched against
(compared with) the Chemicals of Highest Concern (CMC) mass spectral library [1].
Those chemicals not identified with the CMC search with effluent concentrations
above 100 ng/L, were then reversed-searched against the EPA/NIH/NBS mass
spectral library. Peaks with fits of greater than 70% were considered tentatively
identified. For each tentatively identified component, a list of the best mass
spectral library identifications (up to a total of ten identifications) was reported
along with the percent fit values.
3.4.2 Weekly Ambient Water (Grab and Composite) and Outfall (Grab) Samples
Analysis Procedure
The weekly ambient composite and ambient grab samples were analyzed at
two different laboratories, Battelle-Columbus and ERL-D. The outfall water sample
from"the chemical plant was analyzed at ERL-D. The ambient grab samples for
Station 1 were analyzed for dissolved and paniculate chemical concentrations at
Battelle-Columbus.
The analytical methods used at both laboratories were similar, and the
concentrations reported for the target chemicals were comparable between the
two laboratories. Similar data between the two laboratories were obtained by the
use of an internal standard quantification method, 13C- labelled surrogates for
determining compound recoveries, and reporting of the data after recovery
correction.
The analytical procedures consisted of spiking a known sample volume of
water, Battelle, 5.0 L, and ERL-D, 3.6 L, with 13CrHCE, 13C4-HCBD, 13C6-TeCB,
and 13Ce-HCB at concentrations similar to the target chemical concentrations,
Battelle, 600 ng/L, and ERL-D, 200 ng/L. The spiked water samples were
extracted three times using hexane at 60 mL per liter. The hexane was dried using
sodium sulfate, concentrated using a Kuderna-Danish concentrator (K-D) to ca. 10
mL and reduced to 1.0 or 0.10 mL using a gentle stream of nitrogen. These
extracts were spiked with the internal standard, d12-chrysene, Battelle, 2 mg/L and
ERL-O, 10 mg/L.
Dissolved chemical concentrations in this study were defined as the
chemical which passes through a Whatman glass fiber filter. (Note, the exact filter
type was not recorded in laboratory record book.) Particulate chemical
concentrations were defined as the chemical which was retained by the Whatman
25
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glass fiber filter. For the filtered samples from Station 1, ten liter samples were
filtered through Whatman glass fiber filters, (pore size was not recorded) using
vacuum filtration. The filtered ambient samples were analyzed as described above.
The particulates retained on the filters were analyzed by crumbling the filters, then
spiking the filters with the surrogate solution, and finally extracting with two 100
ml aliquots of hexane followed by one 100 ml aliquot of acetone using a tumbling
extractor device. The hexane and acetone were combined, concentrated to
approximately 0.5 ml using a K-D concentrator, and reduced to 100 //L using
natural evaporation. These extracts were spiked with the internal standard, d,2-
chrysene, at a 2 mg/L concentration.
GC/MS analysis using selected ion monitoring (SIM) was performed, and
quantifications were performed using an internal standard method with 5 or 6
standard concentrations. An average response factor (Battelle) or a piece-wise
calibration curve (ERL-D) were used in the quantification of each chemical. The
responses of the surrogate chemicals were corrected for isotopic inferences from
the native chemical responses prior to calculation of their response factors. The
most predominant ion was used to quantify each compound.
Quantification standards contained the internal standard (d12-chrysene), the
fourcarbon-13 labelled surrogates (13CrHCE, 13C4-HCBD, 13C6-TeCB, and 13C6-
HCB), and the native forms of the target chemicals except for the TeCBD and
PeCBD compounds, which were not available commercially. For the TeCBD#1,
TeCBD#2, PeCBD#1, and PeCBD#2 compounds, the response factor for HCBD
was used for quantification. The 1,2,4,5- and 1,2,3,5-TeCBs could not be
consistently resolved on the gas chromatograph and therefore, these two
chemicals were quantified and reported as a mixture.
All quantification results for the site study chemicals were recovery
corrected. For HCE, recovery corrections were made using the recoveries of ^C,-
HCE. For PeCB and HCB, recovery corrections were made using the recoveries of
13C6-HCB. For 1,2,4-TrCB, 1,2,3-TrCB, 1,2,4,5-and 1,2,3,5-TeCB mixture (TeCB
Mix), 1,2,3,4-TeCB, TeCBD#1, TeCBD#2, PeCBD#1, PeCBD#2, and HCBD,
recovery corrections were made using the recoveries of 13Ce-1,2,4,5-TeCB. The
recovery of the 13C4-HCBD surrogate was not used to recovery correct the
TeCBD#1, TeCBD#2, PeCBD#1, PeCBD#2, and HCBD compounds due to the
extremely high concentrations of natural HCBD which masked the responses of the
labelled HCBD on the GC/MS.
Since some of the site study chemicals were recovery corrected using the
recoveries for different compounds, recovery confirmation experiments were
performed using the analysis procedure with reagent water spiked with these
chemicals. Differences in recoveries between PeCB and HCB were 1 %, and
26
-------
between TeCB Mix and 1,2,4-TrCB, 1,2,3-TrCB, 1,2,3,4-TeCB, and HCBD were
small, 23, 17, 14, and 1%, respectively.
Before finalizing the data, surrogate recoveries, inter- and intra-laboratory
consistency, and procedure blank values were evaluated for each sample. An
acceptable range of recoveries for the surrogates was set at 20% to 120% for
water analyses. Site chemicals with surrogate recoveries outside of this range
were rejected as being unreliable. For chemicals failing this evaluation, their
determinations were not reported but were labeled as not passing this quality
control evaluation.
Inter- and intra-laboratory precision of the recovery corrected chemical
concentrations reported was evaluated by comparing replicate determinations. For
replicate samples with chemical concentrations differing by a factor of 4 or more,
sample extracts were reanalyzed on the GC/MS, and when available, an additional
aliquot of sample was prepared and analyzed. Samples with data not consistent
with their reanalysis on the GC/MS were rejected as unreliable. Samples not
consistent with the newly prepared sample extracts were also rejected as
unreliable. The chemical concentrations determined by the reanalysis of the
sample extracts and by the extraction of a new aliquot sample were not reported.
Procedural blanks for each sample set were evaluated for each site study
chemical. Blank concentrations were compared with previous analyses. When
blank concentrations changed by a factor of 4 or more, the procedural blank
extract was reanalyzed on the GC/MS. Blanks with data not consistent with their
reanalysis on the GC/MS were rejected as unreliable. If all of the analytes in the
blank were greater than a factor of 4 from past analyses, the entire sample set
was rejected as unreliable.
For each sampling station, average concentrations were determined for
each chemical after correction for the procedural blank. Correction for the
procedural blank was performed by averaging blank concentrations from all
procedural blank analyses and then subtracting their average blank value from their
reported concentrations. If a blank corrected concentration of less than zero was
obtained, a value of zero was used for that replicate when the average station
concentration was calculated.
3.4.3 Tissue Analysis Procedure
3.4.3.1
Procedures
Compositing and Homogenization
Enough organisms and tissue mass existed for four of the ten species for
making tissue composites. These species were the R heteroclitus. £L. sapidus.
27
-------
between PeCB and 13C6-HCB, 0%, and between 13Ce-1.2,4,5-TeCB and 1,2,4-
TrCB, 1,2,3-TrCB, 1,2,3,4-TeCB, and HCBD, 14%, 1%, 9%, and 4%,
respectively.
3.4.4 Sediment Analysis Procedure
The sediment analysis was performed at ERL-D. Although sediment was
collected from three locations at each station, only one sample (usually the mid-
channel location) was analyzed per station.
Samples were removed from the refrigerator and allowed to warm to room
temperature. Two grams of sediment were weighed out and mixed with enough
anhydrous sodium sulfate to dry the sample. The dried samples were spiked with
the 13C-labelled surrogates, i.e., 13CrHCE, 13C<-HCBD,13C6-TeCBD and 13C6-HCB, at
a concentration of approximately 4.0 //g/g per compound and were Soxhlet
extracted using 1:1 mixture of methylene chloride and hexane. After extraction,
the extracts were passed through columns containing sodium sulfate and activated
copper, and were concentrated using a K-D concentrator to approximately 10 ml.
These extracts were concentrated further to 1.5 ml using a stream of nitrogen
gas. Portions of the 1.5 ml extract were spiked with the internal standard, d12-
chrysene, at concentrations ranging from 0.5 mg/L to 10 mg/L. The spiked
extracts were analyzed using the GC/MS and data review procedures used for the
ambient water samples.
Concurrently with the sediment residue analyses, percent moisture
determinations for sediment samples were performed by drying a 4 to 5 g aliquot
of the sediment in an oven at 105°C for 12 hours.
Concurrently with the sediment residue analyses, total organic carbon
analysis was performed by weighing a 4 or 5 g aliquot of each sample and drying
thenrj at 105°C for 12 hours. The samples were allowed to cool in a desiccator
and then were ground with a mortar and pestle into a fine powder. Approximately
0.3 to 0.5 g of each sample was transferred into a preweighed vial. The samples
were acidified drop wise with 10% HCI until the foaming ceased and returned to
the oven for 12 hours (overnight). The following day, total organic carbon was
measured for each sample by using a Dohrmann TOC Analyzer DC80 with a
DC 183 furnace.
30
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3.4.5 Dissolved and Paniculate Organic Carbon Analysis Procedures
Dissolved, particulate, and total organic carbon (DOC, POC, and TOC,
respectively) were determined for the ambient water samples. Prior to filtration, all
glassware and glass fiber filters were "ashed" in a muffle furnace. A 100 mL
aliquot of a water sample was vacuum-filtered through a pre-moistened 47 mm
(filter diameter) Gelman A/E glass fiber filter (1 //m pore size). The filtrates were
poured into a glass bottle, adjusted to less than a pH of 2 with HCI, capped with a
teflon lined lid, and placed in a refrigerator at 4°C. Between each filtration, the
filtering apparatus was rinsed with Millipore* water. The filter pads were dried in a
covered aluminum pan at 110°C for more than 2 hours. Filter and filtrate blanks
were collected after the processing of every 2 to 3 samples by filtering 100 ml of
Millipore* water through the system. Concurrently with the filtration of the water
samples, whole water samples were poured into a glass bottle, adjusted to less
than a pH of 2 with HCI, capped with a teflon lined lid, and placed into a
refrigerator at 4°C.
The ambient water samples collected on September 19 and 26 were not
acidified (preserved) until the 9th of October, 17 and 11 days after filtration,
respectively.
The filtrates, filters, and whole water samples were analyzed by the
National Spectrographic Laboratories (Cleveland, Ohio) for organic carbon.
Dissolved organic carbon and total organic carbon were measured using the EPA
method 415.1. Particulate organic carbon was measured using a LECO* carbon
analyzer and the ASTM method E350.
3.4.6 Procedures for Particle Sizing the Sediments
A composite sediment sample was prepared for each station by mixing
equal portions of the mid-channel and quarter points. An aliquot of the composite
was treated with H202 to remove organic carbon and the sample was dispersed in
water using a sodium metaphosphate and sodium carbonate solution. The <20
//m, <5 //m, and <0.2 //m fractions were determined by pipetting after
sedimentation and <0.2 //m fraction was determined by pipetting after
centrifugation. The pipetted solutions were dried at 105°C for at least 12 hours
and then weighed. The sand fraction was separated from the silt and clay
fractions by washing a sample through a 300 mesh sieve and the various sand
fractions were determined by sieving and weighing a dried portion (at 105°C) of
the washed sand. The particle sizing procedure was performed by the Ohio State
University, Department of Agronomy, Soil Characterization Laboratory in Columbus
Ohio.
31
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RESULTS AND DISCUSSION
4.1 Expected Tissue Residue Trends
The guidance procedure for predicting residues in aquatic organisms does
not account for metabolism. For this site study, it is believed that the chemicals
under investigation, i.e., chlorinated benzenes, butadienes, and ethanes, are very
slowly metabolized by invertebrate and vertebrate aquatic organisms.
Studies by Nichols et al. [11] and Gargas and Andersen [12] have shown
that hexachloroethane is poorly metabolized in rainbow trout and rats, respectively.
In the review by Matthews [13], pentachlorobenzene and hexachlorobenzene were
reported to be slightly metabolized in rabbits and rats. Bauer et al. [14] and
Sanborn et al. [15] have shown that hexachlorobenzene metabolites can be found
at trace levels in blue mussel and green sunfish when they are exposed to
hexachlorobenzene. Matthews [13] has also reported that for the chlorinated
benzene family, the rate of metabolism by mammalian species was inversely
proportional to the degree of chlorination, i.e., the higher the degree of
chlorination, the less metabolism occurs.
In view of the slow rate of metabolism for these chemicals, the following
general statements about the comparison of the measured and predicted tissue
residues can be made prior to examining the site study data.
a) Agreement between the measured and predicted tissue residues
should be fairly similar for invertebrate and vertebrate organisms.
b) Similar agreement between the measured and predicted tissue
residues should be observed all for chemicals for a given
organism.
c) If any metabolism did occur in the site study organisms, the lower
chlorinated benzenes and butadienes would have poorer
agreement between the measured and predicted tissue residues
than the higher chlorinated congeners .
4.2 Ambient Water Concentrations: Results
Replicate analyses were performed on each ambient water sample for the
thirteen site study chemicals. The individual determinations as well as the
procedural blanks performed with these analyses are reported in Tables A-7
through A-18. In Table 4-1, the weekly and four week average chemical
concentrations are reported for the ambient grab samples taken at each sampling
station, for the ambient composite samples taken at Station 1, and for the grab
samples taken from the outfall of the chemical plant prior to dilution with cooling
wate'r.
32
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Table 4-1. Concentration of Target Chemicals in Weekly Ambient Water Samples from the Canal and Bayou
d'lnde.
Concentration in Effluent na/L*
Coefficient
Std. of
Week 1 Week 2 Week 3 Week 4 Avg. Dev. Variation, %
Hexachloroethane
Outfall
A
1 Composite
1 Grab
B
C
D
E
350
222
46.9
99.0
932
47.2
81.5
51.5
87.2
188
144
237
264
136
198
152
23.1
134
153
138
144
8.67
16.4
8.23
34.0
848
229
1283
681
21.6
874
425
124
348
143
439
505
53.3
292
159
153
335
74.8
566
366
57.2
395
187
124
96.4
52.2
129
72.4
107
135
118
Tetrachlorobutadiene
Outfall
A
1 Composite
1 Grab
B
C
D
E
0.665
13.6
7.56
12.5
18.9
9.77
11.4
7.29
0.00
18.9
8.07
30.4
44.5
24.3
32.0
30.3
0.00
26.2
9.15
8.26
33.0
1.31
2.36
1.05
0.00
94.5
16.6
194
61.0
3.89
137
32.5
0.166
38.3
10.3
61,3
39.3
9.81
45.7
17.8
0.333
37.8
4.22
89.0
17.8
10.3
62.1
16.0
200
98.8
40.8
145
45.4
105
136
89.7
Tetrachlorobutadiene #2
Outfall
A
1 Composite
1 Grab
B
C
D
E
1.04
33.1
17.7
26.6
38.5
23.7
31.9
16.9
2.95
45.3
24.6
73.4
90.3
62.2
71.1
45.6
1.69
49.1
17.5
18.5
67.9
5.33
7.63
7.64
2.79
302
37.8
484
204
13.0
466
102
2.12
107
24.4
151
100
26.1
144
43.0
0.912
130
9.53
224
72.7
25.3
216
42.5
43.1
121
39.1
148
72.1
97.0
150
98.8
Pentachlorobutadiene
Outfall
A
1 Composite
1 Grab
B
C
D
E
7.08
199
70.9
102
275
113
171
96.0
8.44
280
92.6
340
303
123
246
231
5.38
356
64.2
90.2
324
0.00
0.302
0.393
8.50
1503
179
2240
799
16.9
899
369
7.35
584
102
692
425
63.2
329
174
1.47
615
53.0
1040
250
63.7
393
161
20.0
105
52.1
150
58.8
101
120
92.3
33
-------
Table 4-1.
Continued
Concentration in Effluent no/I*
Week 1
Week 2
Week 3
Week 4
Avg.
Std.
Oev.
Coefficient
of
Variation, %
Pentachlorobutadiene #2
Outfall
A
1 Composite
1 Grab
B
C
D
E
3.39
58.2
22.8
37.0
75.3
28.8
37.5
20.5
6.35
73.6
29.6
110
93.4
79.1
109
114
2.99
102
23.3
24.0
118
0.475
1.44
1.75
2.72
530
41.5
821
249
3.89
1610
85.8
3.86
191
29.3
248
134
28.0
439
55.4
1.68
227
87.0
384
78.7
36.3
780
52.9
43.5
119
29.7
155
58.7
129
178
95.5
Outfall
A
1 Composite
1 Grab
B
C
D
E
51.5
389
128
235
745
235
364
224
253
616
397
681
890
385
562
456
83.8
598
407
498
530
0.359
0.894
1.72
173
4280
1540
5040
4780
180
4630
2740
141
1470
618
1610
1740
200
1390
855
91.3
1880
628
2290
2030
159
2170
1270
64.9
128
102
142
117
79.3
156
148
Outfall
A
1 Composite
1 Grab
B
C
D
E
Hexachlorobutadiene
Outfall
A
1 Composite
1 Grab
B
C
D
E
Trichlorobenzene, 1,2
Outfall
A
1 Composite
1 Grab
B
C
D
E
Trichlorobenzene. 1,2
Outfall
A
1 Composite
1 Grab
B
C
D
E
3.39
58.2
22.8
37.0
75.3
28.8
37.5
20.5
51.5
389
128
235
745
235
364
224
,3-
2.02
15.7
16.1
12.4
32.7
26.4
14.8
23.8
,4-
9.95
103
49.8
80.8
164
77.4
106
67.9
Outfall
A
1 Composite
1 Grab
B
C
D
E
2.02
15.7
16.1
12.4
32.7
26.4
14.8
23.8
1.39
20.1
28.0
28.1
33.7
28.4
23.6
22.2
3.35
27.4
28.8
25.7
22.0
5.96
2.01
7.0
2.60
156
42.9
202
194
13.3
182
90.3
2.34
54.8
28.9
67.1
70.7
18.5
55.6
35.8
0.835
67.7
10.9
90.3
82.6
10.7
84.8
37.1
35.7
123
37.8
135
171
58.0
152
103
Outfall
A
1 Composite
1 Grab
B
C
D
E
9.95
103
49.8
80.8
164
77.4
106
67.9
16.1
149
115
216
242
169
180
172
14.6
148
121
104
157
23.8
18.4
14.5
12.8
801
222
1130
685
57.8
1140
458
13.3
300
127
382
312
81.9
360
178
2.63
334
70.9
500
251
61.9
523
198
19.7
111
55.9
131
80.6
75.5
145
111
34
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Table 4-1.
Continued
Concentration in Effluent na/L*
Week 1
Week 2
Week 3
Week 4
Avg.
Std.
Dev.
Coefficient
of
Variation, %
Tetrachlorobenzene Mix
Outfall
A
1 Composite
1 Grab
B
C
D
E
Tetrachlorobenzena,
Outfall
A
1 Composite
1 Grab
B
C
D
E
Pentachlorobenzene
Outfall
A
1 Composite
1 Grab
B
C
D
E
Hexachlorobenzene
Outfall
A
1 Composite*
1 Grab
B
C
D
E
7.63
26.1
13.9
26.5
44.3
24.1
30.3
25.3
1.2.3.4-
3.95
27.6
7.83
26.0
40.2
20.4
26.6
21.2
15.7
40.1
17.9
35.1
72.9
25.2
33.4
31.5
44.6
41.8
16.6
28.9
127
17.9
24.9
28.0
15.2
21.8
27.9
65.2
63.0
53.8
53.9
34.4
5.94
10.9
23.9
28.3
63.6
48.2
56.4
45.2
54.7
52.0
52.0
78.2
76.8
61.1
69.7
56.0
189
31.8
30.1
34.1
35.9
25.9
32.9
26.0
15.6
42.1
74.9
65.4
34.4
25.0
13.5
9.87
10.8
49.2
41.9
41.9
46.7
16.4
11.6
10.1
32.1
46.9
97.4
48.9
56.7
23.0
16.1
13.7
88.0
41.9
77.1
56.5
43.4
17.3
14.2
13.6
12.3
435
129
524
830
41.0
467
361
6.55
347
83.1
471
448
22.2
408
197
27.7
855
148
1160
1190
23.3
544
287
189
739
138
1340
1460
8.26
265
122
12.7
131
61.3
170
243
36.0
141
108
6.81
109
39.2
142
150
26.8
126
68.4
32.5
249
78.8
331
349
33.2
166
97.1
128
214
65.4
364
418
17.4
84.2
47.4
3.68
203
51.9
236
392
14.2
218
169
2.88
159
32.4
220
199
14.5
189
87.0
16.3
404
56.5
554
561
18.7
253
128
72.9
350
54.8
649
699
7.22
121
50.1
29.0
154
84.7
139
161
39.5
154
157
42.4
147
82.7
155
133
54.0
150
127
50.1
163
71.6
167
161
56.3
153
132
57.2
164
83.8
178
167
41.6
143
106
' Recovery and blank corrected
35
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The inter- and intra-laboratory agreement was good for all of the site study
chemicals, e.g., for HCBD at Station B, coefficients of variation were 0.5, 4.4,
12.4, and 25.4% for intra- and 2.8 and 11.7% for inter-laboratory variability. The
average recoveries (coefficients of variation) of the surrogates, 13C,-HCE, 13C6-
TeCB, and 13C6-HCB, were 45.7 (36.6), 45.5 (39.1), and 49.7 (35.0)%,
respectively. The procedural blanks performed with these analyses were
consistently very low at both laboratories for all chemicals. The only exception
was one procedural blank which had an unusually high HCE concentration and very
low concentrations for all of the other chemicals. We believe that this high
determination is an outlier since 10 other procedural blanks performed by this
laboratory were consistent and substantially lower in concentration. Consequently,
this value was not used in the blank correction procedure for determining the
average concentrations reported in Table 4-1.
In Tables A-19 and A-20, the dissolved and particulate chemical
concentrations for the ambient composite samples taken from Station 1 are
reported, respectively. In Tables A-21 and A-22, particulate and dissolved organic
carbon results are reported for the ambient water samples, respectively.
4.3 Ambient Water Concentrations: Discussion
To predict residues in the indigenous organisms, the ambient water
concentration of each chemical must be known and these concentrations must be
relatively constant for a 20 to 40 day period. The best way to estimate the
ambient water concentrations would have been to collect four, seven day 24-hour
composite samples at each of the field sampling stations. However, field
conditions, especially in the bayou, as well as the costs associated with such field
work precluded this type of sampling for this study.
For this study, ambient seven day 24-hour composite samples were
collected at Station 1 only. For the other field stations as well as Station 1,
weekly ambient grab samples were collected. By comparing the composite and
grab samples at Station 1, the representativeness of the grab samples for
establishing the overall ambient water concentrations for the site study could be
evaluated.
Examination of the composite and grab sample data taken at Station 1
(Table 4-1) indicates that for the first three weeks of the site study that the grab
samples provided estimates similar to those obtained by the composite sampling
technique. Differences between the grab and composite chemical concentrations
were less than a factor of 1.8 on average and the individual differences ranged
from 0.5 to 3.8. These composite samples, weeks 1, 2, and 3, were not
completely based upon continuous intermittent sampling. Due to problems with
the ISCO* sampler, some of the composite sample collected each day were brought
36
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to volume using a grab sample taken that same day. In Table A-23, the
percentages of composite versus grab samples in the seven day composites are
reported. This equipment problem introduces some bias into the ambient water
concentrations reported for the composite samples.
In contrast to weeks 1, 2, and 3, the grab and composite samples for the
fourth week were markedly different. The ambient concentrations reported for the
grab sample were always higher than the composite sample concentrations,
sometimes by an order of magnitude. In addition, the water concentrations for the
grab and composite samples were higher than the concentrations observed for the
previous three weeks. Differences in reported ambient concentrations between
weeks 3 and 4 ranged from a factor of 7.8 to 34 for the grab samples, and 1.5 to
3.8 for the composite samples.
In comparing the grab samples from the canal and bayou sampling stations
for weeks 3 and 4, elevated chemical concentrations observed at these field
stations suggest that the grab sample from Station 1 wasn't an outlier. The whole
field site appears to have elevated ambient concentrations during the fourth week
of the study, Table 4-1. The only site where an elevated grab sample doesn't exist
was the outfall for the chemical plant prior to dilution with cooling water.
The establishment of the 28 day "steady-state" exposure concentrations for
the six field stations presents some difficulty due to the unusually high grab sample
concentrations in the fourth week. In this investigation, we decided to determine
the 28 day "steady-state" exposure concentration by averaging the four weekly
concentrations for each station since none of the data could be dismissed as being
not representative. Furthermore, by including on an equal basis all four weeks of
data, naturally occurring variability for this site is included. The bayou is
influenced by storm and wind surges off the Gulf of Mexico, by the tides, runoff
passing through Bayou d'lnde, and changes in discharge flow and its composition.
• By using the four week arithmetic average for the 28 day exposure
concentration, some error exists in the derived average exposure concentrations.
The dynamics of this tidally influenced receiving water are complex, and should not
be overlooked.
4.4 Sediment Results
For each field station, the concentrations of the site study chemicals in the
sediments were measured. In general, the canal sediments were highly
contaminated and bayou sediments were less contaminated, Table 4-2. The
organic carbon content and particle size data for the sediments are reported in
Tables A-24 and A-25.
37
-------
Table 4-2. Concentration of Target Chemicals in Sediment Samples from the
Canal and Bayou d'lnde.
TOC'
Station Percent
Hexachloroethaneb
Blank
Blank
A
1
B
C
D
E
0.00
0.00
7.19
3.69
9.12
2.99
4.14
5.31
Percent
Moisture
0.00
0.00
75.8
72.9
80.6
60.3
69.5
69.9
Surrogate
% Recovery
78.2
83.0
100
43.5
41.7
82.3
92.1
81.6
Target Chemical
Recovery Corrected
[ng/g]
206
218
1090
199
266
157
210
183
(1.09//g/g)
Tetrachlorobutadiene #1C
Blank
Blank
A
1
B
C
D
E
0.00
0.00
7.19
3.69
9.12
2.99
4.14
5.31
0.00
0.00
75.8
72.9
80.6
60.3
69.5
69.9
86.2
91.1
97.3
91.4
86.9
91.6
87.2
95.2
0.00
0.00
233
70.6
124
0.00
3.71
0.00
Tetrachlorobutadiene #2C
Blank
Blank
A
1
B
C
D
E
0.00
0.00
7.19
3.69
9.12
2.99
4.14
5.31
0.00
0.00
75.8
72.9
80.6
60.3
69.5
69.9
86.2
91.1
97.3
91.4
86.9
91.6
87.2
95.2
0.00
0.00
3150
955
2070
4.50
29.3
15.1
(3.15//g/g}
(2.07 fjg/g)
38
-------
Table 4-2. Continued.
Station
TOC'
Percent
Percent
Moisture
Surrogate
% Recovery
Target Chemical
Recovery Corrected
[ng/g]
Pentachlorobutadiene #1C
Blank
Blank
A
1
B
C
D
E
0.00
0.00
7.19
3.69
9.12
2.99
4.14
5.31
0.00
0.00
75.8
72.9
80.6
60.3
69.5
69.9
86.2
91.1
97.3
91.4
86.9
91.6
87.2
95.2
0.00
0.00
17300 (17.3 /yg/g)
3920 (3.92 /yg/g)
2920 (2.92 /yg/g)
6.50
44.8
23.7
Pentachlorobutadiene #2C
Blank
Blank
A
1
B
C
O
E
0.00
0.00
7.19
3.69
9.12
2.99
4.14
5.31
0.00
0.00
75.8
72.9
80.6
60.3
69.5
69.9
86.2
91.1
97.3
91.4
86.9
91.6
87.2
95.2
0.00
0.00
2380 (2.38 /yg/g)
354
365
0.00
4.87
2.52
Hex^chlorobutadiened
Blank
Blank
A
1
B
C
O
E
0.00
0.00
7.19
3.69
9.12
2.99
4.14
5.31
0.00
0.00
75.8
72.9
80.6
60.3
69.5
69.9
86.2
91.1
97.3
91.4
86.9
91.6
87.2
95.2
12.9
18.7
591000(591 /yg/g)
301000(301 /yg/g)
245000(245 /yg/g)
62.1
1110 (1.11 /yg/g)
249
39
-------
Table 4-2. Continued.
TOC'
Station Percent
Trichlorobenzene. 1
Blank
Blank
A -
1
B
C
D
E
Trichlorobenzene. 1
Blank
Blank
A
1
B
C
D
E
Tetrachlorobenzene
Blank
Blank
A
1
B
C
D
E
.2.3-c
0.00
0.00
7.19
3.69
9.12
2.99
4.14
5.31
.2.4-c
0.00
0.00
7.19
3.69
9.12
2.99
4.14
5.31
Mixc
0.00
0.00
7.19
3.69
9.12
2.99
4.14
5.31
Percent
Moisture
0.00
0.00
75.8
72.9
80.6
60.3
69.5
69.9
0.00
0.00
75.8
72.9
80.6
60.3
69.5
69.9
0.00
0.00
75.8
72.9
80.6
60.3
69.5
69.9
Surrogate
% Recovery
86.2
91.1
97.3
91.4
86.9
91.6
87.2
95.2
86.2
91.1
97.3
91.4
86.9
91.6
87.2
95.2
86.2
91.1
97.3
91.4
86.9
91.6
87.2
95.2
Target Chemical
Recovery Corrected
[ng/g]
8.88
0.523
2080
368
747
8.01
28.4
9.67
0.00
0.00
21500
5310
12100
59.9
529
232
12.7
12.5
24800
8330
10600
22.6
145
88.9
(2.08 fjg/g)
(21.5 fjg/g)
(5.31 fjg/g)
(12.1 ^g/g)
(24.8 fjg/g)
(8.33^g/g)
(10.6 fjg/g)
40
-------
Table 4-2. Continued.
Station
TOC'
Percent
Percent
Moisture
Surrogate
% Recovery
Target Chemical
Recovery Corrected
[ng/g]
Tetrachlorobenzene. 1.2.3.4-°
Blank
Blank
A
1
B
C
D
E
0.00
0.00
7.19
3.69
9.12
2.99
4.14
5.31
0.00
0.00
75.8
72.9
80.6
60.3
69.5
69.9
86.2
91.1
97.3
91.4
86.9
91.6
87.2
95.2
0.00
0.00
17400 (17. 4 //g/g)
5520 (5. 52 /yg/g)
2940 (2.94 /yg/g)
9.27
100
43.9
Pentachlorobenzened
Blank
Blank
A
1
B
C
D
E
0.00
0.00
7.19
3.69
9.12
2.99
4.14
5.31
0.00
0.00
75.8
72.9
80.6
60.3
69.5
69.9
93.8
95.9
67.9
32.8
59.6
97.9
85.2
93.8
0.00
0.00
152000(1 52 /yg/g)
167000(1 67 /yg/g)
48200 (48. 2 //g/g)
0.00
1100 (1.10 /yg/g)
272
Hexachlorobenzened
Blank
Blank
A
1
B
C
D
E
' TOC =
b 13f* |_if*
0.00
0.00
7.19
3.69
9.12
2.99
4.14
5.31
total organic carbon.
C 01 irrs\sio+a
0.00
0.00
75.8
72.9
80.6
60.3
69.5
69.9
93.8
95.9
67.9
32.8
59.6
97.9
85.2
93.8
4.18
5.53
156000(156 //g/g)
656000(656 /yg/g)
246000(246 /yg/g)
56.2
7690 (7. 69 /yg/g)
1810 (1.81 /yg/g)
v*1 i iv^i- oui i uyaio.
13C6 TeCB surrogate.
13
C8 HCB surrogate.
41
-------
4.5 Tissue Data: Results
Replicate analyses were performed on each tissue composite for the thirteen site
study chemicals. The individual determinations as well as the procedural blanks
performed these analyses are reported in Tables A-27 through A-62 for the
E. heteroclitus. £i sapidus. EL oatronus. and M. undulus species. In Tables 4-3, 4-
4, 4-5, and 4-6, the average residue concentrations after correction for procedural
blanks and normalization to 7.6% lipid content for each species are reported for
each station.
The inter- and intra-laboratory agreement was good for all of the site study data,
e.g., for C_. saoidus. the coefficients of variation for HCBD were 52.8, 11.0, 14.1,
12,8, and 56.8% for intra- and 9.8, 7.21, 25.4, and 4.4% for inter-laboratory
variability. The average recoveries (coefficients of variation) of the surrogates,
13CrHCE, 13C8-TeCB, and 13C8-HCB, were 38.6 (36.0), 33.0 (37.3), and 36.1
(35.9)%, respectively. The procedural blanks performed with these analyses were
consistently low at both laboratories for all chemicals and species.
4.6 Prediction of the Tissue Residues
To evaluate the residue prediction procedure, residues were predicted and then
compared to the measured residues for the indigenous organisms. By using the
residue prediction procedures, values for the log P, BCF, FM, and BAF were derived
for qach chemical, Table 4-7. For HCE, 1,2,4-TrCB, 1,2,3-TrCB, 1,2,3,4-TeCB,
PeCB, HCB, and HCBD, measured log P values were used [16, 16, 17, 18, 17, 17,
19, respectively). For the TeCB Mix, a mixture of 1,2,4,5- and 1,2,3,5-TeCBs, the
average of the measured log P value for each compound was used [18]. For the
PeCBD#1, PeCBD#2, TeCBD#1, and TeCBD#2 compounds, log P values were
derived by using the CLogP program [20] with the buta-1,3-diene structure. For
the TeCBDs and PeCBDs, the average of the CLogP log P values for the 5 and 2
possible isomers were used, respectively.
Residues were predicted for the Louisiana site by using the derived BAFs (Table
4-7) and the average ambient water chemical concentrations. The predicted tissue
residues are reported in Tables 4-8, 4-9, 4-10, and 4-11.
42
-------
Table 4-3. Concentration of Target Chemicals in Fundulus heteroclitus
from Cooling Water/Effluent Canal and Bayou d'lnde.
Concentration in Tissue, A/g/kg"
ssb avgb sdb cv,%b nb ss avg sd cv,% n ss avg sd cv,% n
Hexachloroethane
A 471
1 925
B 688
C 788
D 176
E 533
97.0
-
245
-
143
-
20.6
-
35.7
-
80.9
-
Pentachlorobutadiene
A 1270
1 1660
B 2720
C 902
D 594
E 430
1130
-
2040
-
885
-
88.7
-
74.8
-
149
-
4
1
5
1
3
1
11
4
1
5
1
3
1
Trichlorobenzene. 1.2.3-
Tetrachlorobutadiene #1
A
1
B
C
D
E
30.4
31.0
54.0
23.7
13.3
13.3
16.5
-
36.4
-
21.4
-
54.2
-
67.3
-
161
-
Pentachlorobutadiene
A
1
B
C
D
E
221
360
663
205
144
100
361
-
506
-
240
-
163
-
76.2
-
166
-
4
1
5
1
3
1
£2
4
1
5
1
3
1
Trichlorobenzene. 1.2.4-
Tetrachlorobutadiene #2
A 137 36.8
1 206
B 183 61.3
C 133
D 38.5 29.6
E 87.6
26.9
-
33.5
-
76.9
-
4
1
5
1
3
1
Hexachlorobutadiene
A289001 5400
1 69800
B3800Q21600
C68100
D 8580 5480
E34100
53.4
-
55.6
-
63.9
4
1
5
1
3
Tetrachlorobenzene Mix
A 122 25.4 20.8 4
1 266 - - 1
B 226 70.6 31.3 5
C 182 - - 1
D 47.5 31.6 66.5 3
E 142 - - 1
A 807 199 24.6 4
1 1350 - - 1
B 1240 303 24.4 5
C 2670 - - 1
D 280 155 55.4 3
E 872 - - 1
A 1290 846 65.5 4
1 2770 - - 1
B 1750 1170 66.6 5
C 9.45 - - 1
D 499 215 43.1 3
E 1790 - - 1
Tetrachjorobenzene. 1
A 717
1 1160
B 1240
C 1050
D 371
E 734
151
-
361
-
219
-
21.1
-
29.2
-
59.0
-
.2.3.4- Pentachlorobenzene
4
1
5
1
3
1
A 4720 1010
1 8210
B 78804820
C 7980
D 1740 897
E 3990
21.4
-
61.2
-
51.5
-
4
1
5
1
3
1
Hexachlorobenzene
A
1
B
C
D
E
5400
6430
4650
5960
1220
1370
2930
-
2880
-
317
-
54.3
-
62.0
-
26.1
-
4
1
5
1
3
1
Recovery, blank and lipid corrected. 7.6% lipid content.
ss = sampling station, avg = average, sd = standard deviation, cv,% =
coefficient of variation in percent, n = number of tissue samples analyzed.
43
-------
Table 4-4. Concentration of Target Chemicals in Callinectes sapidus from
Cooling Water/Effluent Canal and Bayou d'lnde.
Concentration in Tissue, /yg/kg"
ss'
avgb sdb cv,%b nb ss avg sd cv,% n ss avg sd cv,% n
Hexachloroethane
A 16.8
1 11.0
B 18.6
C 19.8
D 10.0
E 15.3
6.77
3.07
4.52
14.5
1.68
8.21
40.2
27.8
24.2
73.2
16.9
53.6
Pentachlorobutadiene
A 306
1 219
B 459
C 328
D 80
E 309
74.3
78.8
219
464
28.4
216
69.8
36.0
47.7
141
35.6
69.8
Trichlorobenzene. 1.2
A 81.5
1 166
B 267
C 172
D 91.1
E 138
45.7
78.0
154
189
29.5
83.7
56.1
47.0
57.7
110
32.4
60.6
Tetrachlorobenzene, 1
A 368
1 706
B 928
C 692
D 368
E 497
122
294
625
654
188
292
33.1
41.6
67.4
94.5
51.2
58.8
3
4
2
2
2
3
11
3
4
2
2
2
3
,3-
3
4
2
2
2
3
.2.3.4-
3
4
2
2
2
3
Tetrachlorobutadiene
A 6.20
1 16.5
B 33.5
C 29.8
D 7.85
E 24.4
7.10
16.4
8.19
42.1
1.04
15.2
115
99.0
24.5
141
13.3
62.3
Pentachlorobutadiene
A 1.81
1 7.24
B 10.3
C 10.7
D 3.04
E 4.86
3.13
3.46
9.51
15.2
1.03
4.80
173
47.8
92.0
141
33.9
98.7
Trichlorobenzene. 1.2
A 308
1 713
B 1455
C 967
D 416
E 706
209
315
652
1220
84.2
349
67.8
44.3
44.8
126
20.2
49.4
II
3
4
2
2
2
3
12
3
4
2
2
2
3
A:
3
4
2
2
2
3
Pentachlorobenzene
A 1467
1 2473
B 2348
C 1630
D 987
E 1239
278
845
1500
1380
717
787
19.0
34.2
63.9
84.7
72.7
63.5
3
4
2
2
2
3
Tetrachlorobutadiene #_2
A 21.9
1 64.3
B 170
C 103
D 52.2
E 80.4
5.22
30.3
82.8
132
15.2
36.2
23.8
47.1
48.8
128
29.2
45.0
3
4
2
2
2
3
Hexachlorobutadiene
A 274
1 340
B 728
C 482
D 261
E 363
160
270
259
682
175
200
58.6
79.6
35.6
141
67.1
55.2
3
4
2
2
2
3
Tetrachlorobenzene Mix
A 476
1 849
B 1450
C 1049
D 571
E 682
85.1
524
1210
933
332
468
17.9
61.7
83.2
89.0
58.1
68.6
3
4
2
2
2
3
Hexachlorobenzene
A 1772
1 2093
B 1486
C 868
D 781
E 1052
131
624
943
594
713
734
7.37
29.8
63.5
68.5
91.3
69.7
3
4
2
2
2
3
"Recovery, blank and lipid corrected. 7.6% lipid content.
bss = sampling station, avg = average, sd = standard deviation, cv,% =
coefficient of variation in percent, n = number of tissue samples analyzed.
44
-------
Table 4-5. Concentration of Target Chemicals in Brevoortia patronus from
Cooling Water/Effluent Canal and Bayou d'lnde.
Concentration in Tissue, #g/kg*
ssb avgb sdb cv,%b nb ss avg sd cv,% n ss avg sd cv,% n
Hexachtoroethane
C 222 108 48.7 7
D 391 225 57.5 2
E 374 149 39.7 4
Pentachlorobutadiene #1
C 2440 1210 49.5 7
D 5320 2950 55.5 2
E 5370 1970 36,8 4
Tetrachlorobutadiene #1
C 46.0 19.7 42.9 7
D 79.7 53.0 66.5 2
E 91.3 30.1 33.0 5
Pentachlorobutadiene f 2
C 530 246 46.4 7
D 1090 640 58.8 2
E 1020 354 34.6 4
Tetrachlorobutadiene f 2
C 132 60,1 45.5 7
D 235 136 57.6 2
E 237 75.6 31,9 4
Hexachlorobutadieng
C 85103460 40.7 7
D 159006100 38.5 2
E 166005850 35.2 4
Trichlorobenzene. 1.2.3-
C 113 34.2 30.3 7
D 165 91.4 55.4 2
E 201 46.0 22.9 4
Trichlorobenzgnf, 1.2.4-
C 669 262 39.2 7
D 1150 684 59.6 2
E 1200 315 26.1 4
Tetrgchlorobenzene, 1^2,3,4-
C 646 249 38.5 7 C
D 1070 574 53.6 2 D
E 1170 442 37.6 4 E
Pentachlorobenzene
3040 1130 37.1 7
5140 1500 29.2 2
5320 2670 50.2 4
Tetrachlorobenzene Mix
C 497 162 32.6 7
D 862 382 44.4 2
E 887 331 37.3 4
Hexachlorobenzene
C 1950 530 27.1 7
D 3510 670 19.1 2
E 49204520 91.9 4
* Recovery, blank and lipid corrected. 7.6% lipid content.
bss = sampling station, avg = average, sd = standard deviation, cv,% =
coefficient of variation in percent, n = number of tissue samples analyzed.
45
-------
Table 4-6. Concentration of Target Chemicals in Microoogan undulus from
Cooling Water/Effluent Canal and Bayou d'lnde.
Concentration in Tissue, //g/kg"
ssb avgb sdb cv,%b nb ss avg sd cv,% n ss avg sd cv,% n
Hexachloroethane
B 85.2 1
C 48.7 1
D 241 53.1 22.1 2
E 326 1
Pentachlorobutadiene #1
B 858 1
C 401 1
D 4040 400 9.90 3
E 5000 1
Trichlorobenzene. 1.2,3-
B 81.4 1
C 27.0 1
D 210 94.8 45.1 3
E 218 1
Tetrachlorobutadiene #1
B 19.2 1
C 6.35 1
D 81.50.965 1.18 3
E 102 1
Pentachlorobutadiene #2
B 92.2 1
C 35.5 1
D 426 118 27.7 3
E 443 1
Trichlorobenzene. 1.2.4-
A 356 1
B 142 1
C 1160 241 20.9 3
D 1290 1
Tetrachlorobutadiene #2
B 65.6 1
C 20.2 1
D 203 1.50 0.7373
E 276 1
Hexachlorobutadiene
B 5600 1
C 4180 1
D 206007820 37.9 3
E 17300 1
Tetrachlorobenzene Mix
B 309 1
C 248 1
D 810 242 29.9 3
E 788 1
Tetrachlorobenzene. 1,2.3,4- Pentachlorobenzene
B 339
C 303
D 1020 322
E 948
1
1
31.6 3
1
B 1972
C 1820
D 3860 910
E 3360
1
1
23.6 3
1
Hexachlorobenzene
B 2200 1
C 2100 1
D 2890 800 27.7 3
E 2500 1
• Recovery, blank and lipid corrected. 7.6% lipid content.
bss = sampling station, avg = average, sd = standard deviation, cv,% =
coefficient of variation in percent, n = number of tissue samples analyzed.
46
-------
Table 4-7. Residue Prediction Parameters.
Compound
Hexachloroethane
Tetrachlorobutadiene #1
Tetrachlorobutadiene #2
Pentachlorobutadiene #1
Pentachlorobutadiene #2
Hexachlorobutadiene
1,2,3- Trichlorobenzene
1,2,4- Trichlorobenzene
Tetrachlorobenzene Mix
1,2,3,4- Tetrachlorobenzene
Pentachlorobenzene
Hexachlorobenzene
logP
4.14
3.22
3.22
3.71
3.71
4.78
4.05
4.02
4.87
4.64
5.17
5.31
BCF
742
140
140
340
340
2380
630
597
2800
1840
4830
6240
FM
1
1
1
1
1
1
1
1
1
1
3
3.7
BAF
742
140
140
340
340
2380
630
597
2800
1840
14500
23100
47
-------
Table 4-8. Predicted and Measured Fundulus heteroclitus Tissue Concentrations
for the Louisiana Study.
Average
Cone, in
Ambient Water
Station
Hexachloroethane
A
1 Composite
1 Grab
B
C
D
E
Tetrachlorobutadiene
A *
1 Composite
1 Grab
B
C
D
E
Tetrachlorobutadiene
A
1 Composite
1 Grab
B
C
D
E
(ng/L)
348
143
439
505
53.3
292
159
11
38.3
10.3
61.3
39.3
9.81
45.7
17.8
12
107
24.4
151
100
26.1
144
43.0
Predicted*
Cone, in
Tissue
U/g/kg)
258
106
326
375
39.5
217
118
5.36
1.44
8.58
5.50
1.37
6.40
2.46
15.0
3.42
21.1
14.0
3.65
20.2
6.02
Observed6
Cone, in
Tissue
(^g/kg)
471
925
688
788
176
533
30.4
31.0
54.0
23.7
13.3
13.3
137
206
183
133
38.5
87.6
Ratio of
Observed to
Predicted
Cone, in
Tissue
1.82
2.84
1.84
19.9
0.812
4.52
5.67
3.61
9.81
17.3
2.08
5.34
9.15
9.74
13.1
36.4
1.93
14.6
48
-------
Table 4-8. Continued.
Average Predicted"
Cone, in Cone, in
Ambient Water Tissue
Station (ng/L) U/g/kg)
Pentachlorobutadiene
A
1 Composite
1 Grab
B
C
D
E
Pentachlorobutadiene
A
1 Composite
1 Grab
B
C
D
E
Hexachlorobutadiene
A
1 Composite
1 Grab
B
C
D
E
11
584
102
692
425
63.2
329
174
12
191
29.3
248
134
28.0
439
55.4
1470
618
1610
1740
200
1390
855
199
34.7
235
145
21.5
112
59.2
64.9
10.0
84.3
45.6
9.52
149
18.8
3499
1471
3832
4141
476
3308
2035
Ratio of
Observed6 Observed to
Cone, in Predicted
Tissue Cone, in
(X'g/kg) Tissue
1270
1660
2720
902
594
430
221
360
663
205
144
100
28900
69800
38000
68100
8580
34100
6.40
7.06
18.8
42.0
5.31
7.27
3.40
4.27
14.6
21.5
0.965
5.31
8.26
18.2
9.18
143
2.59
16.8
49
-------
Table 4-8. Continued.
Average Predicted'
Cone, in Cone, in
Ambient Water Tissue
Station (ng/L) (/vg/kg)
Trichlorobenzene, 1,2.3-
A
1 Composite
1 Grab
B
C -
D
E
Trichlorobenzene, 1,2.4-
A
1 Composite
1 Grab
B
C
D
E
TetrachlofQbjgnzene Mix
A
1 Composite
1 Grab
B -
C
D
E
54.8
28.9
67.1
70.7
18.5
55.6
35.8
300
127
382
312
81.9
360
178
131
61.3
170
243
36.0
141
108
34.5
18.2
42.3
44.5
11.7
35.0
22.6
179
75.8
228
186
48.9
215
106
367
172
476
681
101
395
303
Observed6
Cone, in
Tissue
(/yg/kg)
122
266
226
182
47.5
142
807
1350
1240
9.45
280
872
1290
2770
1750
2670
499
1790
Ratio of
Observed to
Predicted
Cone, in
Tissue
3.53
6.29
5.07
15.6
1.36
6.30
4.51
5.92
6.66
0.193
1.30
8.21
3.52
5.82
2.57
26.5
1.26
5.92
50
-------
Table 4-8. Continued.
Average Predicted"
Cone, in Cone, in
Ambient Water Tissue
Station (ng/L) U/g/kg)
Tetrachlorobenzene, 1
A
1 Composite
1 Grab
B
C
D
E
Pentachlorobenzene
A
1 Composite
1 Grab
B
C
D
E
Hexachlorobenzene
A
1 Composite
1 Grab
B
C
D
E
.2.3.4-
109
39.2
142
150
26.8
126
68.4
249
78.8
331
349
33.2
166
97.1
214
65.4
364
418
17.4
84.2
47.4
201
72.1
261
276
49.3
232
126
3610
1140
4800
5060
481
2400
1410
4940
1510
8400
9650
402
1940
1090
Observed6
Cone, in
Tissue
0/g/kg)
717
1160
1240
1050
371
734
4720
8210
7880
7980
1740
3990
5400
6430
4650
5960
1220
1370
Ratio of
Observed to
Predicted
Cone, in
Tissue
3.57
4.44
4.49
21.3
1.60
5.83
1.31
1.71
1.56
16.6
0.723
2.84
1.09
0.765
0.482
14.8
0.628
1.25
7.6% lipid content.
7.6% lipid content and corrected for recovery and procedural blank.
51
-------
Table 4-9. Predicted and Measured Callinectes sapid us Tissue Concentrations for
the Louisiana Study.
Average
Cone, in
Ambient Water
Station
Hexachloroethane
A
1 Composite
1 Grab
B
C
D
E
Tetrachlorobutadiene
A
1 Composite
1 Grab
B
C
D
E
Tetrachlorobutadiene
A
1 Composite
1 Grab
B
C
D
E
(ng/L)
348
143
439
505
53.3
292
159
11
38.3
10.3
61.3
39.3
9.81
45.7
17.8
12
107
24.4
151
100
26.1
144
43.0
Predicted"
Cone, in
Tissue
U/g/kg)
258
106
326
375
39.5
217
118
5.36
1.44
8.58
5.50
1.37
6.40
2.49
15.0
3.42
21.1
14.0
3.65
20.2
6.02
Observed11
Cone, in
Tissue
bg/kg)
16.8
11.0
18.6
19.8
10.0
15.3
6.20
16.5
33.5
29.8
7.85
24.4
21.9
64.3
170
103
52.2
80.4
Ratio of
Observed to
Predicted
Cone, in
Tissue
0.0651
0.0338
0.0496
0.501
0.0462
0.130
1.16
1.92
6.09
21.7
1.23
9.79
1.46
3.04
12.1
28.2
2.59
13.4
52
-------
Table 4-9. Continued.
Average Predicted"
Cone, in Cone, in
Ambient Water Tissue
Station (ng/L) (^g/kg)
Pgntachlorobutadiene
A
1 Composite
1 Grab
B
C
D
E
Pentachlorobutadiene
A
1 Composite
1 Grab
B
C
D
E
Hexachlorobutadiene
A
1 Composite
1 Grab
B
C
D
E
jn
584
102
692
425
63.2
329
174
12
191
29.3
248
134
28.0
439
55.4
1470
618
1610
1740
200
1390
855
199
34.7
235
145
21.5
112
59.2
64.9
10.0
84.3
45.6
9.52
149
18.8
3499
1471
3832
4141
476
3308
2035
Observed6
Cone, in
Tissue
(^g/kg)
106
219
459
328
80
309
1.81
7.24
10.3
10.7
3.04
4.86
274
340
728
482
261
363
Ratio of
Observed to
Predicted
Cone, in
Tissue
0.534
0.931
3.18
15.3
0.715
5.22
0.0279
0.0859
0.226
1.12
0.0204
0.258
0.0783
0.0887
0.176
1.01
0.0789
0.178
53
-------
Table 4-9. Continued.
Average Predicted*
Cone, in Cone, in
Ambient Water Tissue
Station (ng/L) (//g/kg)
Trichlprobenzene, 1,2,3-
A
1 Composite
1 Grab
B
C
D
E
Triphlorobenzene. 1.2.4-
A
1 Composite
1 Grab
B
C
D
E
Tgtrachlorobenzene Mix
A
1 Composite
1 Grab
B
C
D
E
54.8
28.9
67.1
70.7
18.5
55.6
35.8
300
127
382
312
81.9
360
178
131
61.3
170
243
36.0
141
108
34.5
18.2
42.3
44.5
11.7
35.0
22.6
179
75.8
228
186
48.9
215
106
367
172
476
681
101
395
303
Observed6
Cone, in
Tissue
U>g/kg)
81.5
166
267
172
91.1
138
308
713
1460
967
416
7O6
476
849
1450
1050
571
682
Ratio of
Observed to
Predicted
Cone, in
Tissue
2.36
3.93
5.99
14.8
2.60
6.12
1.72
3.13
7.84
19.8
1.94
6.66
1.30
1.78
2.13
10.4
1.45
2.25
54
-------
Table 4-9. Continued.
Station
Tetrachlorobenzene.
A
1 Composite
1 Grab
B
C
D
E
Pentachlorobenzene
A
1 Composite
1 Grab
B
C
D
E
Hexachlorobenzene
A
1 Composite
1 Grab
B
C
D
E
Average
Cone, in
Ambient Water
(ng/L)
1.2,3,4-
109
39.2
142
150
26.8
126
68.4
249
78.8
331
349
33.2
166
97.1
214
65.4
364
418
17.4
84.2
47.4
Predicted"
Cone, in
Tissue
U/g/kg)
201
72.1
261
276
49.3
232
126
3610
1140
4800
5060
481
2400
1410
4940
1510
8400
9650
402
1940
1090
Observed15
Cone, in
Tissue
(//g/kg)
368
706
928
692
368
497
1470
2470
2350
1630
987
1240
1770
2090
1490
868
781
1050
Ratio of
Observed to
Predicted
Cone, in
Tissue
1.83
2.70
3.36
14.0
1.59
3.95
0.407
0.515
0.465
3.39
0.410
0.881
0.358
0.249
0.154
2.16
0.402
0.959
' 7.6% lipid content.
" T dOjL \\r\ij-t f+r\ r>+«r\+ **r\f\ f+r** rmf*+af4 fr\r rfkf+r\\ifkr\i ^nr4 r\rr\f+£*f4» ir*s\ fr\lo n I/
55
-------
Table 4-10. Predicted and Measured Brevoortia patronus Tissue Concentrations for
the Louisiana Study.
Average
Cone, in
Ambient Water
Station (ng/L)
Hex^chloroethane
C
D
E
Tetrachjorobutadiene
C
D
E
Tetrachjorobutadiene
C
D
E
Pentachlorobutadiene
C
D
E
Pentachlorobutadiene
C
D
E
Hexachlorobutadiene
C
D
E
53.3
292
159
£1
9.81
45.7
17.8
£2
26.1
144
43.0
Jl
63.2
329
174
12
28.0
439
55.4
200
1390
855
Predicted"
Cone, in
Tissue
(^g/kg)
39.5
217
118
1.37
6.40
2.49
3.65
20.2
6.02
21.5
112
59.2
9.52
149
18.8
476
3308
2035
Observed1*
Cone, in
Tissue
Ovg/kg)
222
391
374
46.0
79.7
91.3
132
235
237
2440
5320
5370
530
1090
1020
8510
15900
16600
Ratio of
Observed to
Predicted
Cone, in
Tissue
5.61
1.80
3.17
33.5
12.5
36.6
36.1
11.7
39.4
114
47.6
90.8
55.7
7.30
54.2
17.9
4.81
8.16
56
-------
Table 4-10. Continued.
Average Predicted'
Cone, in Cone, in
Ambient Water Tissue
Station (ng/L) (//g/kg)
Trichlorobenzene. 1.2.3-
C
D
E
Trichlorobenzene. 1.2.4-
C
D
E
Tetrachlorobenzene Mix
C
D
E
Tetrachlorobenzene. 1.2.
C
D
E
Pentachlorobenzene
C
D
E
Hexachlorobenzene
C
D
E
18.5
55.6
35.8
81.9
360
178
36.0
141
108
3,4-
26.8
126
68.4
33.2
166
97.1
17.4
84.2
47.4
11.7
35.0
22.6
48.9
215
106
101
395
303
49.3
232
126
481
2400
1410
402
1940
1090
Observed11
Cone, in
Tissue
(X/g/kg)
113
165
201
669
1150
1200
497
862
887
646
1070
1170
3040
5140
5320
1950
3510
4920
Ratio of
Observed to
Predicted
Cone, in
Tissue
9.70
4.71
8.91
13.7
5.35
11.3
4.93
2.18
2.93
13.1
4.62
9.30
6.32
2.14
3.78
4.85
1.81
4.50
7.6% lipid content.
lipid content and corrected
recovery and procedural blank.
57
-------
Table 4-11. Predicted and Measured Micropogan undulus Tissue Concentrations for
the Louisiana Study.
Average
Cone, in
Ambient Water
Station
Hexachloroethane
B
C
D
E
Tetrachlorobutadiene
B
C
D
E '
Tetrachlorobutadiene
B
C
0
E
Pentachlorobutadiene
B
C
D
E
Pentachlorobutadiene
B
C
D
E
(ng/L)
505
53.3
292
159
£1
39.3
9.81
45.7
17.8
12
100
26.1
144
43
_£1
425
63.2
329
174
J2
134
28.0
439
55.4
Predicted'
Cone, in
Tissue
l/ig/kg)
375
39.5
217
118
5.50
1.37
6.40
2.49
14.0
3.65
20.2
6.02
145
21.5
112
59.2
45.6
9.5
149
18.8
Observed*1
Cone, in
Tissue
U/g/kg)
85.2
48.7
241
326
19.2
6.35
81.5
102.0
65.6
20.2
203.0
276.0
858
401
4040
5000
92.2
35.5
426
443
Ratio of
Observed to
Predicted
Cone, in
Tissue
0.227
1.23
1.11
2.76
3.49
4.62
12.7
40.9
4.69
5.53
10.1
45.8
5.94
18.7
36.1
84.5
2.02
3.73
2.85
23.5
58
-------
Table 4-11. Continued.
Average Predicted* Observed6
Cone, in Cone, in Cone, in
Ambient Water Tissue Tissue
Station (ng/L) U/g/kg) U/g/kg)
Hgxachlorobutadiene
B 1
C
D 1
E
Trichlorobenzene. 1.2.3-
B
C
D
E
Trichlorobenzene. 1.2.4-
B
C
D
E
Tetrachlorobenzene Mix
B
C
D
E
Tetrachlorobenzene. 1.2.
B
C
D
E
740
200
390
855
70.7
18.5
55.6
35.8
312
81.9
360
178
243
36.0
141
108
3,4-
150
26.8
126
68.4
4141
476
3308
2035
44.5
11.7
35.0
22.6
186
48.9
215
106
681
101
395
303
276
49
232
126
5600
4180
20600
17300
81.4
27.0
210
218
356
142
1160
1290
309
248
810
788
339
303
1020
948
Ratio of
Observed to
Predicted
Cone, in
Tissue
1.35
8.78
6.23
8.50
1.83
2.32
6.00
9.67
1.91
2.90
5.40
12.1
0.454
2.46
2.05
2.60
1.23
6.14
4.40
7,53
59
-------
Table 4-11. Continued.
Station
Average
Cone, in
Ambient Water
(ng/L)
Predicted*
Cone, in
Tissue
l^g/kg)
Observed1"
Cone, in
Tissue
-------
4.7 Comparison of the Predicted and Observed Tissue Residues: Results
In Table 4-12, all of the tissue predictions based upon the ambient grab
water samples were tabulated according to the ratio of the observed to predicted
tissue residues.
For the £_., sapidus. 32, 42, and 53 of the 72 predicted residues for all
chemicals were within a factor of 3, 5, and 10, respectively, of the observed
residues. For predicted residues differing by a factor of 5 or more, approximately
half were smaller than the measured residues. In general, better predictability was
observed for the tetra- through hexa-chlorobenzenes, i.e., 17 of the 24 predicted
residues were within a factor of 3, and 21 of the 24 predicted residues were
within a factor of 5 of the measured residues. The poorest predictability was
observed for HCE and HCBD where only of 2 of the 12 predicted residues were
within a factor of 5 of the measured tissue residues. The field stations where the
best predictability was observed were A and D, i.e., 10 of the 12 predicted
residues for each station were within a factor of 3. Stations C and E tended to
predict tissue residues which were much smaller than the observed residues.
For the L. heteroclitus. 23, 33, and 55 of the 72 predicted residues for all
chemicals were within a factor of 3, 5, and 10 of the observed residues,
respectively. All of the remaining predicted residues, except for one, were smaller
than-the measured residues. The best predictability was observed for HCE, PeCB
and HCB where 4, 5, and 5 of the 6 predicted residues, respectively, were within a
factor of 3 of the measured tissues. The poorest predictability was observed for
the chlorinated butadienes where 1 or less of the 6 predicted residues were within
a factor of 3 at each station. The field stations where the best predictability was
observed were A, 1, and D. The poorest predictability was observed at stations B
and C and predictions were smaller than the measured residues by factors ranging
up to 42.
For the Mi undulus. 20, 28, and 35 of the 48 predicted residues were within
a factor of 3, 5, and 10 of the observed residues, respectively. The remaining
predicted residues were all too small by factors ranging up to 84.5. Predicted
residues for HCE, TeCB mix, PeCB and HCB were all within a factor of 5 (16 of 16
predictions) and the predicted residues for HCBD, 1,2,3-TrCB, and 1,2,3,4-TeCB
were all within a factor of 10. Stations B, C, D, and E had 7, 6, 5, and 4 of the
12 predicted residues within a factor of 3, respectively, and had 11, 8, 6, and 4 of
the 12 residues with a factor of 5, respectively.
For the EL. patronus. 5, 13, and 21 of the 36 predicted residues were within
a factor of 3, 5, and 10 of the observed residues, respectively. The best
predictability was observed with the HCE, TeCB Mix, 1,2,3,4-TeCB, PeCB and
HCB compounds. For the rest of the chemicals, 14 of the 36 predicted residues
61
-------
Table 4-12 Distribution of the Ratios of the Observed to Predicted Tissue
Concentrations for All Field Stations.
Factor"
of 3
It
eq
gt
Factor6
of 5
It
eq
gt
Factor6
of 10
It
eq
gt
Total Number
of Tissue
Predictions
Hexachloroethane
C. sapidus
F.-heteroclitus
M. undulus
B. patronus
All fishes
5
0
1
0
1
1
4
3
1
8
0
2
0
2
4
5
0
0
0
0
1
5
4
2
11
0
1
0
1
2
4
0
0
0
0
2
5
4
3
12
0
1
0
0
1
6
6
4
3
13
Chlorinated Butadienes
C. sapidus 1
F. heteroclitus
M. undulus
B. patronus
All fishes
0
0
0
0
0
10
4
3
0
7
10
26
17
15
58
8
0
0
0
0
14
7
7
1
15
8
23
13
14
50
6
0
0
0
0
19
19
8
3
30
5
11
12
12
35
30
30
20
15
65
Chlorinated Benzenes
All
C. saoidus
F. heteroclitus
M. undulus
B. patronus
All fishes
Chemicals
C. sapidus 1
F. heteroclitus
M. undulus
B. patronus
All fishes
2
1
1
0
2
7
1
2
0
3
21
15
14
4
33
32
23
20
5
481
13
20
9
14
43
23
48
26
31
05
1
1
0
0
1
14
1
0
0
1
27
21
17
10
48
42
33
28
13
74
8
14
7
8
29
16
38
20
23
81
0
0
0
0
0
10
0
0
0
01
32
31
23
15
69
53
55
35
21
11
4
5
1
3
9
9
17
13
15
45
36
36
24
18
78
72
72
48
36
156
' It = Number of ratios < 1/3; eq = Number of ratios > 1/3 and < 3; gt =
Number of tissue residue ratios > 3.
b It = Number of ratios < 1/5; eq = Number of ratios ^1/5 and s 5; gt =
Number of tissue residue ratios > 5.
0 It = Number of ratios < 1/10; eq = Number of ratios > 1/10 and s 10; gt
Number of tissue residue ratios > 10.
62
-------
were too small by a factor of 10 or more and the poorest predictability was
observed at Stations C and E.
Overall, the guidance residue prediction procedure had slightly better
predictability for the invertebrate, £,. saoidus than for the fishes, F. heteroclitus. EL
patronus. and M. undulus. The guidance procedure tended to have a skewed
predictability for the fishes, i.e., substantially more of the predicted residues were
smaller than the measured residues. The residues predicted using the guidance
procedure were in better agreement for Stations A and D, and in poorest
agreement at Stations C and E. The predicted and measured residues for the
highly chlorinated benzenes, TeCB Mix, 1,2,3,4-TeCB, PeCB, and HCB, were in
good agreement for all species at nearly of all stations. The predicted and
measured residues for HCE were in good agreement for the fishes but in poor
agreement for the invertebrate, (L. sapid us. at nearly all stations.
4.8 Comparison of the Predicted and Observed Tissue Residues: Discussion
The predicted tissue residues derived using the guidance procedure tended to
be, on average, smaller than the concentrations measured in the site study
organisms. Prediction of tissue residues which were smaller than the observed
residues might be caused by the elevated ambient water concentrations in the
fourth week of the study and/or the use of inaccurate log P values with the
guidance procedure.
As previously discussed, the ambient water concentrations for the site study
chemicals were not constant during the 28 day study. During the fourth week of
the study, chemical concentrations in the ambient water samples were up to an
order of magnitude larger than the concentrations observed in the previous three
weeks of the study.
In this study, the indigenous organisms were collected in the fourth week, the
time of the highest ambient water concentrations. For some of the site study
chemicals, aquatic organisms reach or approach steady-state conditions with their
expo.sure water in relatively short time periods. For example, Konemann and van
Leeuwen [21] have shown with Poecilia reticulata (guppy) that di- and tri-
chlorobenzenes reach steady-state conditions within 2 days, and TeCBs, PeCB and
HCB reach steady-state conditions in approximately 7 days. It can be estimated by
using the third and fourth week concentrations in the ambient grab samples and
assuming that an abrupt change in the ambient water concentrations occurred at
Station 1, e.g., HCB concentrations went from 56.5 to 1340 ng/L, that a time
period of approximately 0.5 to 1.5 days of higher exposure concentrations, e.g.,
1340 ng/L, could have existed (based upon HCBD, PeCB, and HCB data). This
short period of higher exposure concentration just before collection of the
63
-------
organisms would cause the observed residues to be slightly larger than predicted
residues. The ratios of the observed to predicted tissue concentrations for the
chlorjnated benzenes are consistent with this hypothesis since their ratios tend to
decrease with decreasing chemical uptake rate in all four species, e.g., 1,2,3-TrCB
should and does have, on average, larger ratios than HCB.
The tendency of the predicted tissue residues to be smaller than the observed
residues may also be, in part, caused by the use of inaccurate log P values with
the guidance technique. The guidance procedure uses a log P - log BCF
relationship to estimate the BCF for each chemical and then uses this value to
predict the tissue residue. Therefore, any uncertainty/inaccuracy in the log P
values are directly reflected in the predicted tissue residues.
For the chlorinated benzenes, numerous high quality log P measurements exist.
For the chlorinated butadienes, a few log P measurements exist only for HCBD. In
this study, we used estimated log P values for the TeCBDs and PeCBDs, and the
uncertainties associated with these values could be large. For the HCBD, the
estimated and measured log P values are 4.3 [20] and 4.78 [19], respectively.
This difference suggests that estimated log P values for the chlorinated butadienes
in all likelihood are too small. In addition, a field measured BAF for HCBD of 3580
[22] is also slightly larger than the predicted BAF of 2380 for HCBD which
suggests that the log P values are, possibly, too low for the chlorinated
butadienes. The ratios of the observed to predicted residues (Table 4-12) shows
that the tendency to predict residues which are too small are greater for the
chlorinated butadienes than for the chlorinated benzenes and this trend is
consistent with the quality of the log P values used in the residue predictions.
The tendency to predict residues which were smaller than the observed residues
can not be solely attributed to the quality of the log P values used with the
guidance technique. Overall, the log P values for the chlorinated benzenes were of
high quality and approximately 12% of the predicted residues for this chemical
class were too small by a factor of 10 or more.
Variability associated with the ambient exposure concentrations used in the
prediction of the tissues might also be, in part, a cause of the tendency to predict
residues which were too small for the site study chemicals. Based upon the
hydrodynamics of the field site, greater changes/fluctuations in ambient
concentrations would be expected at the bayou sampling stations due to tides and
run-off. In Table 4-13, the distribution of the ratios of the observed to predicted
tissue residues for the canal sampling stations are reported. As expected,
substantially better predictability was obtained for the canal sampling stations,
e.g.,"all of the residues predicted for the chlorinated benzenes were within a factor
of 10 or less.
64
-------
The residue prediction procedure provided particularly poor estimates for
Station C. Station C was located above the confluence of the bayou and the canal
and had the lowest ambient water chemical concentrations of all of the stations.
This station should have had the lowest observed tissue residues in the study.
However, tissue residues measured at station C were very similar to those at
stations D and E in the bayou. The mobility of the aquatic organisms at this site is
unknown. However, the tissue data suggests that the organisms were fairly
mobile in the bayou during the time of the site study since fairly similar tissue
concentrations were observed for all three bayou sampling stations. If station C is
not included in the tabulation of the ratios of the observed to predicted tissue
residues (Table 4-14), the number of predicted residues which were too small
decreases substantially, e.g., 24% to 13%. Even with C station not included in
the tabulation, the poorest predictability was still observed for the chlorinated
butadienes.
The chemicals predicted to be larger than their measured tissue concentrations
for the C. sapidus, in general, were HCE, PeCBDs, and HCBD. In contrast, no
chemicals were predicted to be larger for the fishes, e.g., none of 156 predicted
tissue concentrations were greater than their measured residues by a factor of 10
or more (Table 4-12).
The measured ambient water concentrations were expected to be more
representative of the exposure conditions for the fishes since these samples were
taken from the middle of the water column at each sampling station. The C.
sapidus live on the sediments and their exposure via the water could be different
from that observed at the middle of the water column. Ground water intrusion from
the marshes surrounding the canal and bayou, salinity gradients due to the tides,
thermal gradients caused by the discharge, and/or runoff could cause differences in
ambfent water concentrations between the middle of the water column and the
sediment/water interface. These differences might account for the tissue
predictions which were much larger than the measured concentrations. For
example, the sediments contain nondetectable amounts of HCE and therefore, the
C. sapidus might have lower tissue concentrations for HCE due to lower ambient
water concentrations at the sediment/water interface.
The measured and predicted tissue concentrations were in agreement with the
expected trends for metabolic behavior of the site study chemicals. In Section 4.1,
similar agreement between the measured and predicted tissue residues a) for all
chemicals for a given organism and b) for both invertebrate and vertebrate
organisms were forecasted. Similar levels of agreement did occur for these
chemicals for each organism as well as among the fishes and C. sapidus. The only
chemicals with divergent behavior were HCE, PeCDBs, and HCBD for the C.
sapidus. Exposure conditions, specific metabolic abilities and/or special
65
-------
Table 4-13 Distribution of the Ratios of the Observed to Predicted Tissue
Concentrations for Field Stations A, 1-G, and B
Factor'
Factor6
of 3
Hexachloroethane
C. sapidus
F. heteroclitus
M. undulus
B. patronus
All fishes
Chlorinated Butadienes
C. sapidus
F. heteroclitus
M. undulus
B. patronus
All fishes
Chlorinated Benzenes
C. sapidus
F. heteroclitus
M. undulus
B. patronus
All fishes
All Chemicals
C. sapidus 1
F. heteroclitus
M. undulus
B. patronus
All fishes
' It = Number of ratios
It
3
0
1
0
1
6
0
0
0
0
2
0
1
0
1
1
0
2
0
2
<
Number of tissue residue
b It = Number of ratios
<
Number of tissue residue
c It = Number of ratios
eq
0
3
0
0
3
5
0
2
0
2
11
7
5
0
12
16
10
7
0
17
1/3
gt
0
0
0
0
0
4
15
3
0
18
5
11
0
0
11
9
26
3
0
29
; eq
ratios >
1/5
; eq
ratios >
It
3
0
0
0
0
5
0
0
0
0
1
0
0
0
0
10
0
0
0
0
of 5
Factor0 Total
of 10
eq gt
0
3
1
0
4
8
3 1
4
0
7 1
15
13
6
0
19
23
19 1
11
0
30 1
= Number
3.
= Number
5.
0
0
0
0
0
2
2
1
0
3
2
5
0
0
5
4
7
1
0
8
of
of
It
3
0
0
0
0
4
0
0
0
0
0
0
0
0
0
7
0
0
0
0
ratios
ratios
eq
0
3
1
0
4
10
11
5
0
16
18
18
6
0
24
28
32
12
0
44
2.
;>
< 1/10; eq = Number of ratios 2
Number of tissue residue
ratios >
10.
gt
0
0
0
0
0
1
4
0
0
4
0
0
0
0
0
1
4
0
0
4
1/3 and
1/5 and
Number
of Tissue
Predictions
1
1
3
3
1
0
4
5
5
5
0
20
1
8
18
6
0
24
36
36
1
2
0
48
^ 3;
^ 5;
; 1/10 and ^
gt =
gt =
10; gt =
66
-------
Table 4-14 Distribution of the Ratios of the Observed to Predicted Tissue
Concentrations for Field Stations A, 1-G, B, D, and E.
Factor"
of 3
It eq gt
Hexachloroethane
C. saoidus 500
F. heteroclitus 0 4 1
M. undulus 1 2 0
B. patronus 011
All fishes 1 7 2
Chlorinated Butadienes
C. saoidus 10 8 7
F. heteroclitus 0 4 21
M. undulus 0 3 12
B. oatronus 0 0 10
All fishes 0 7 43
Chlorinated Benzenes
C. saoidus 2 20 8
F. heteroclitus 0 15 15
M. undulus 1 11 6
B. patronus 048
All fishes 1 30 29
All Chemicals
C. sapidus 17 28 15 1
F. heteroclitus 0 23 37
M. undulus 2 16 18
B. patronus 0 5 19
All fishes 2 44 74
' It = Number of ratios < 1/3; eq =
Number of tissue residue ratios > 3.
b It = Number of ratios < 1/5; eq =
Number of tissue residue ratios > 5.
c It = Number of ratios < 1/10; eq =
Number of tissue residue ratios > 10
Factor6
of 5
It eq gt
500
050
030
020
0 10 0
8 12 5
0 7 18
0 5 10
0 1 9
0 13 37
1 25 4
0 21 9
0 13 5
084
0 42 18
4 37 9
0 33 27
0 21 15
0 11 13
0 65 55
Number of
Number of
Factor0 Total Number
of 10
It eq gt
4 1 0
050
030
020
0 10 0
6 17 2
0 19 6
087
037
0 30 20
0 30 0
0 30 0
0 17 1
0 11 1
0 58 2
10 38 2
0 54 6
0 28 8
0 16 8
0 98 22
ratios > 1/3 and
ratios > 1/5 and
of Tissue
Predictions
5
5
3
2
10
25
25
15
10
50
30
30
18
12
60
60
60
36
24
120
* 3; gt =
<: 5; gt =
= Number of ratios > 1/10 and s 10; gt =
67
-------
physiological abilities, i.e., C. sapidus have anaerobic metabolism pathways, might
be responsible for the observed differences for these four chemicals.
4.9 Summary
Six chemicals identified in the grab samples from Station 1 and the outfall using
the effluent analytical method produced chemical residues in the receiving water
fishes and C. sapidus. Seven additional chemicals, found in the sample extracts
prepared by using the effluent analytical method and which were similar to those
identified by the effluent method, also produced chemical residues in the receiving
fishes and C. sapidus.
The guidance technique predicted tissue concentrations which were smaller than
the measured concentrations by a factor of 1.1 and 5.3 on average (geometric
average) for the C. sapidus and fishes, respectively. For the C. sapidus, 32, 42,
and 53 of 72 predicted tissue residues were within a factor of 3, 5, and 10 of the
measured tissue concentrations, respectively (Table 4-12). For the fishes, 48, 74,
and 111 of 156 predicted tissue residues were within a factor of 3, 5, and 10,
respectively (Table 4-12).
The guidance technique provided more accurate tissue concentrations for
chemicals with the highest quality log P values and with the least variable
exposure concentrations. The best predictability was observed for the chlorinated
benzenes (chemicals with the highest quality log P values) at the canal sampling
stations, the field stations with the smallest variability in exposure concentrations.
The poorest predictability was observed for the chlorinated butadienes (chemicals
with the lowest quality log P values) at Station C, the field station upstream of the
confluence of the bayou and canal.
The measured and predicted tissue concentrations were in agreement with the
expected trends for metabolic behavior for the site study chemicals for the fishes.
For the C. sapidus, the measured and predicted tissue concentrations except for
HCE, PeCBDs, and HCBD were in agreement with their expected metabolic
behavior. The HCE, PeCBDs, and HCBD were divergent from their expected
metabolic behavior in that their measured concentrations were substantially lower
than the predicted tissue concentrations suggesting that special metabolic,
exposure, and/or physiological conditions might be controlling bioaccumulation of
these chemicals in C. sapidus.
Field studies, by their inherent nature, include the dynamics and variabilities
associated with natural aquatic systems. The predictability demonstrated at this
tidal site includes the variabilities associated with the natural system, e.g. tides,
highly contaminated sediments, non-sessile organisms, and run off events, and
uncertainties associated with the predictive technique, e.g., uncertainties
associated with the log P values, the log P - BCF relationship, and the food chain
multipliers.
68
-------
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[1] United States Environmental Protection Agency, Office of Water Enforcement
and Permits. Assessment and control of bioconcentratable contaminants in
surface waters. Public Comment Draft (Fed. Regist. 1991, 56, 13150-
13151), March 1991.
[2] Racca, L. 1991. Louisiana Department of Environmental Quality, Personal
Communication.
[3] United States Environmental Protection Agency, Water Quality Management
Branch, Region 6. 1990. Toxics Study of the Lower Calcasieu River.
[4] Pereira, W.E., C.E. Rostad, C.T. Chiou, T.I. Brinton and L.B. Barber II. 1988.
Contamination of estuaries, water, biota, and sediment by halogenated
" organic compounds: A field study. Environmental Science and Technology,
22: 772-778.
[5] Demcheck, O.K., C.R. Demas and C.R. Garrison. 1990. Chemical, tissue,
and physical data from water and bottom material in the lower Calcasieu
River, Louisiana, 1985-88. United States Geological Survey, Open-Filp
Report 89-420.
[6] Veith, G.D. and P. Kosian. 1982. Estimating bioconcentration potential
from octanol/water partition coefficients, in Physical Behavior of PCBs in the
Great Lakes. MacKay, Paterson, Eisenreich, and Simmons, Eds., Chapter 15,
Ann Arbor Science, Ann Arbor, Ml.
[7] Schuurmann, G. and W. Klein. 1988. Advances in bioconcentration
prediction. Chemosphere, 17(8): 1551-1574.
[8] Thomann, R.V. 1989. Bioaccumulation model of organic chemical
distribution in aquatic food chains. Environmental Science and Technology,
23: 699-707.
[9] Thomann, R.V. 1987. A statistical model of environmental contaminants
using variance spectrum analysis. Report to National Science Foundation.
August 1987. NTIS #: PB88-235130/A09.
[10] Rasmussen, J.B., D.J. Rowan, D.R.S. Lean, and J.H. Carey. 1990. Food
chain structure in Ontario lakes determines PCB levels in lake trout (Salvelinus
namaycush) and other pelagic fish. Canadian Journal of Fisheries Science,
47: 2030-2038.
[11] Nichols, J.W., J.M. McKim, G.J. Lien, A.D. Hoffman and S.L. Bertetsen.
1991. Physiologically based toxicokinetic modeling of three waterborne
69
-------
chloroethanes in rainbow trout Oncorhynchus mvkiss. Toxicology and
Applied Pharmacology, 110: 374-389.
[12] Gargas, M.L. and Andersen, M.E. 1989. Determining kinetic constants of
chlorinated ethane metabolism in the rat from rates of exhalation.
Toxicology and Applied Pharmacology, 99: 344-353.
[13] Matthews, H.B. 1982. Aryl halides. In Metabolic basis of detoxification:
Metabolism of functional groups. Jakoby, Bend and Caldwell, Editors.
Chapter 3. Academic Press, New York, NY.
[14] Bauer, I., S. Weigelt and W. Ernst. 1989. Biotransformation of
hexachlorobenzene in the blue mussel Mvtilus edulis. Chemosphere,
19(10/11): 1701-1707.
[15] Sanborn, J.R., W.F. Childers and L.G. Hansen. 1977. Uptake and
elimination of [14C]hexachlorobenzene (HCB) by the green sunfish, Lepomis
cyanellus Raf., after feeding contaminated food. Journal Agricultural Food
and Chemistry, 25(3): 551-553.
[16] Chiou, C.T. 1985. Partition coefficients of organic compounds in lipid water
" systems and correlation with fish bioconcentration factors. Environmental
Science and Technology, 19(1): 57-62.
[17] Watarai, H., M. Tanaka, and N. Suzuki. 1982. Determination of partition
coefficients of halobenzenes in heptane/water and 1-octanol/water systems
and comparison with the scaled particle calculation. Analytical Chemistry,
54(4): 702-705.
[18] Konemann, H., R. Zelle, F. Busser and W.E. Hammers. 1979. Determination
of log P^ values of chloro-substituted benzenes, toluenes and anilines by
high-performance liquid chromatography on ODS-silica. Journal of
Chromatography, 178: 559-565.
[19] Banarjee, S., S.H. Yalkowsky and S.C. Valvani. 1980. Water solubility and
octanol/water partition coefficients of organics. Limitations of the solubility-
partition coefficient correlation. Environmental Science and Technology,
14(10): 1227.
[20] Leo, A. and D. Weininger. 1984. CLog P version 3.2 User Reference
• Manual. Pomona Medicinal Chemistry Project, Pomona College, Claremont,
CA 91711.
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[21] Konemann, H. and K. van Leeuwen. 1980. Toxicokinetics in fish:
Accumulation and elimination of six chlorobenzenes by guppies.
Chemosphere, 9: 3-19.
[22] Oliver, B.G. and A.J. Niimi. 1983. Bioconcentration of chlorobenzenes from
water by brook trout. Correlations with partition coefficients and
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291.
71
-------
APPENDIX A
72
-------
Table A-l. Bioconcentratable Chemicals Tentatively Identified Using the Effluent Analytical Procedure: Outfall, Fraction 1.
NBS/EPA/NIH TENTATIVE IDENTIFICATIONS /chem/msd/90c911601016.d
PeakRT
8.756
9.514
10713
11.186
11.476
14.508
15.859
16.ZS8
16.990
17.221
18.550
19.848
22.568
24.226
(Fit)
97
96
95
91
74
93
95
94
94
93
76
76
86
81
95
89
86
90
87
83
81
78
90
90
86
78
87
Height
Amount (ng/L)
(Name)
73916 182.07
Benzene, U-dichloro- (9CI)
Benzene, 1,4-dichloro- (9CI)
Benzene, 1,3-dichloro- (9CI)
138398 325.87
Ethane, hexachloro- (8CI9CI)
48924 126.33
Benzene, (2-chloroethyl)- (8CI9CI)
28443 8124
13-Butadicnc, pentachloro- (9CI)
101974 244.64
Naphthalene (ACN) (DOT) (8CI9CI)
IH-Indene, 1-racthylcne- (9CI)
Azulene (8CI9CI)
3881660 10766.62
Naphthalene, 2-ethcnyl- (9CI)
l.l'-Biphenyl (9CI)
43474
Acenaphthene (SCI)
114.18
77577 190.23
Dibcnzofuran (8CI9CI)
100639 241.66
Benzene, l-chloro-2-phenoxy- (9CI)
212867 491.94
Benzene, l-chloro-4-phenony- (9CI)
Benzene, l-chloro-3-phenoxy- (9CI)
306136 699.93
Benzene, bromophcnoxy- (9CI)
71340 176.32
Phenanthrene (8C19CI)
Benzene, l,l'-(l,2-ethynediyl)bis- (9CI)
Anthracene (8CI9CI)
9H-Fluorene, 9-mcthylene- (9CI)
2-Cyclopropen-l-one, 24-diphenyl- (9CI)
153846 360.32
1,2-Benzencdicarbatylicacid, bis(2-methoxyethyl) ester (9CI)
1,2-Benzencdicarboxylicacid, butyl cyclohexylester (9CI)
1.2-BenzencdicarboxyIicacid, butyl 2-mcthylpropyl ester (9CI)
1,2-Benzencdicartagtylicacid, butyl 8-mcthylnonyl ester (9CI)
48495 125.38
Fluoranthcnc (8C19CI)
A-1
-------
Table A-2. Bioconcentraiable Chemicals Tentatively Identified Using the Effluent Analytical Procedure: Outfall, Fraction 2.
NBS/EPA/NIH TENTATIVE IDENTIFICATIONS /chem/msd/90c911601017.d
Peak RT Height Amount (ng/L)
(Fit) (Name)
11.233
12.098
15.651
16.352
20.052
24.232
25.080
99
99
99
94
95
90
72
96
87
394254 362.25
1,3-Butadiene, penuchloro- (9CI)
1090000 101X20
1,3-Butadlene, l.l,2J,4,4,-hewchloro- (8CI9CI)
272065 252.21
2^-Cyclohexadiene-l,4-dione,2,6-bis(l,l-dimeuTyteuTyt>-(9CI)
94122 91.95
Benzene, pemachloro- (8CI9CI)
99228 96.55
Benzene, l-l'-
-------
Table A-3. Bioconcentrauble Chemicals Tenutively Identified Using the Effluent Analytical Procedure: Outfall, Fraction 3.
NBS/EPA/NIH TENTATIVE IDENTIFICATIONS /chem/tnsd/90c911601Q18.d
Peak RT Height Amount (ng/L)
(Fit) (Name)
12.070
18J07
23.987
24.779
31.999
99
98
89
93
72
72
460690 203.13
13-Butadiene, 1,1,23,4,4,-hexachloro- (8CI9CI)
652558 285.62
Benzene, hcxachloro- (8CI9CI)
14-CyetopenUdiene, li3,4-tetrachloro-5-(dichloroinethyleneH8CI9CI)
1539990 1052.43
Sulfur, rool. (S8) (8CI9CI)
271343
Mirex
121.72
156969 72J5
U-BenzenedJcartoarylic acid, 3-nitro- (9CI)
A-3
-------
Table A-4 Bioconcemratable Chemicals Tentatively Identified Using the Effluent Analytical Procedure: Station 1, Fraction 1
NBS/EPA/NIH TENTATIVE IDENTIFICATIONS /chem/msd/90c911601022.d
Peak RT
8.048
8.320
11.400
(Fit)
72
97
97
96
94
87
87
Height
Amount (ng/L)
(Name)
227053 151.17
Pyridine, 2,3.4,5-tetrahydro- (8CI9CI)
301839 199.07
Benzene, 1,4-dichloro- (9CI)
Benzene, 13-dichloro- (9CI)
Benzene, 1.2-dichloro- (9CI)
116305 80.23
Benzene, 1,2,4-trichloro- (8CI9CI)
Benzene, 1,2,3-trichloro- (8CI9CI)
Benzene, 13.5-trichloro- (8CI9CI)
11.501 102574 71.44
91 Azulene (8CI9CI)
91 IH-Indene, 1-methylene- (9CI)
90 Naphthalene (ACN) (DOT) (8CI9CI)
14.463 1541280 1169.58
90 Naphthalene, 2-«thenyl- (9CI)
81 l.r-Biphenyl (9CI)
A-4
-------
Table A-S Bioconccntratable Chemicals Tentatively Identified Using the Effluent Analytical Procedure: Station 1, Fraction 2.
NBS/EPA/Nm TENTATIVE IDENTIFICATIONS /chem/msd/90c911601023.d
Peak RT Height Amount (ng/L)
(Fit) (Name)
11.208 391250 18439
98 1,3-Butadienc, pentachloro- (9CI)
12.071 292527 139.43
99 U-Buudicne, l,lA3,M."hexachloro- (8CI9CI)
1S.646 640694 298.69
99 2^-Cycloheudiene-l,4-dione.2,6-bu(l,l-diinethylethyl)- (9CI)
A-5
-------
Table A-6. Bioconcemratabte Chemicals Tentatively Identified Using the Effluent Analytical Procedure: Station 1, Fraction 3
NBS/EPA/NIH TENTATIVE IDENTIFICATIONS /chem/msd/90c911601024.d
Peak RT
8.107
12.040
23.889
(Fit)
72
99
93
91
Height
(Name)
Amount (ng/L)
307900 113.68
Cyclopropane, 1,1,2,2-tetramethyl- (8CI9CI)
539792 195.64
U-Butadiene, 1,1,2,3,4,4,-hexachloro- (8CI9CI)
9532070 400.37
Sulfur, mol. (S8) (8CI9CI)
Maneb (ACN)
A-6
-------
Table A-7. Concentrations of Hexachloroethane in Ambient Water Samples.
Procedure Blanks
Battglle
ERL-D
Outfall
ERL-D
Station A
Battelle
Week of
Sample
Collection
A
B
C
D
E
F
G
H
1
J
K
L
M
N
a
b
c
d
1-a
1-a
2-b
3-c
4-d
1-A
1-G
1-B
2-D
2-M
3-E
3-N
4-K
4-K
Surrogate
Recovery6
%
R-L
71.5
34.7
R-L
23.8
58.5
57.9
28.0
25.5
R-L
61.3
74.4
48.6
26.2
72.9
87.0
66.8
86.8
33.4
33.7
36.0
38.4
51.0
34.7
R-L
21.6
22.4
39.2
R-L
35.0
R-L
24.1
Concentration
(Recovery Corrected)
[ng/L]
3.95
3.53
4.91
1.17
4.46
2.21
2.26
1.66
3.28
5.27
84.1
3.80
4.00
3.82
5.06
330
378
91.3
27.3
38.2
203
206
167
246
137
865
A-7
-------
Table A-7. Continued.
Week of
Sample
Collection
ERL-D
Station 1 - Composite
Battelle
ERL-D
Station 1 - Grab
Battelle
ERL-D
Station B
Battelle
1-a
2-b
3-c
4-d
1-A
2-C
3-E
1-a
2-b
3-c
4-d
1-B
1-B
2-E
2-N
3-F
3-L
4-J
4-J
1-a
2-b
3-c
4-d
1-C
1-H
2-D
2-M
3-E
3-N
4-J
4-J
Surrogate
Recovery6
%
37.9
47.3
42.7
78.8
38.7
21.0
R-L
40.7
45.7
54.1
59.9
38.7
R-L
R-L
26.8
R-L
R-l
32.6
39.7
37.5
41.4
44.9
58.7
46.4
45.7
30.3
45.4
54.2
42.4
R-l
R-l
Concentration
(Recovery Corrected)
[ng/L]
267
161
138
838
48.8
131
52.5
164
158
233
104
245
1320
1200
102
237
142
1340
774
1080
283
243
137
158
A-8
-------
Table A-7. Continued.
ERL-D
Station C
Battelle
ERL-D
Station D
Battelle
ERL-D
Week of
Sample
Collection
1-a
2-b
3-c
4-d
4-d
1-C
1-H
2-D
2-N
3-L
4-I
4-I
1-a
2-b
3-c
3-c
4-d
1-A
1-H
1-B
2-D
2-M
3-F
3-L
4-K
4-K
1-a
2-b
2-b
3-c
4-d
Surrogate
Recovery''
%
39.1
47.4
R-H
61.2
63.4
97.6
43.0
40.7
48.7
R-l
R-l
R-l
34.2
47.9
53.5
34.4
70.4
R-L
77.1
51.6
31.5
67.2
28.7
R-l
29.1
34.4
43.9
46.4
R-H
40.5
67.5
Concentration
(Recovery Corrected)
[ng/L]
954
276
789
582
52.3
47.5
176
101
52.5
141
13.9
11.8
25.8
76.3
85.8
233
162
23.4
1210
1350
93.0
208
16.7
73.2
A-9
-------
Table A-7. Continued.
Station E
Battelle
ERL-D
Week of
Sample
Collection
1-A
1-H
2-C
2-M
3-F
3-L
4-I
4-I
1-a
2-b
3-c
4-d
Surrogate
Recovery1'
%
R-L
35.8
47.7
26.6
29.6
22.3
R-l
R-l
39.7
R-P
41.7
59.3
Concentration
(Recovery Corrected)
[ng/L]
62.3
122
188
7.69
18.2
48.2
9.52
429
The letter following the week is the procedural blank done with the analysis
of the sample.
Surrogate: 1,2,4,5-13C, HCE, R-L: rejected, recovery <20%, R-H:
• rejected, recovery > 120%, R-l: rejected, incorrect data, R-P: rejected,
procedural error in sample preparation.
A-10
-------
Table A-8. Concentrations of Tetrachlorobutadiene #1 in Ambient Water
Samples.
Procedure Blanks
Battelle
ERL-D
Outfall
ERL-D
Station A
Battelle
Week of
Sample
Collection
A
B
C
D
E
F
G
H
J
I
K
L
M
N
a
b
c
d
1-a
1-a
2-b
3-c
4-d
1-A
1-G
1-B
2-D
2-M
3-E
3-N
4-K
4-K
Surrogate
Recovery1"
%
R-L
45.0
33.6
29.8
39.4
51.9
45.7
34.2
R-L
29.1
55.4
53.9
40.6
37.6
88.2
106
84.3
93.0
39.9
39.7
40.5
52.3
53.7
33.6
29.3
20.9
28.7
29.1
21.1
31.7
R-L
22.7
Concentration
(Recovery Corrected)
[ng/L]
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
1.33
0.00
0.00
0.00
0.00
20.2
4.60
21.7
17.3
30.6
46.7
25.1
142
A-11
-------
Table A-8. Continued.
Week of
Sample
Collection
ERL-D
Station 1 - Composite
Battelle
ERL-D
Station 1 - Grab
Battelle
ERL-D
Station B
Battelle
1-a
2-b
3-c
4-d
1-A
2-C
3-E
1-a
2-b
3-c
4-d
1-B
1-B
2-E
2-N
3-F
3-L
4-J
4-J
1-a
2-b
3-c
4-d
1-C
1-H
2-D
2-M
3-E
3-N
4-J
4-J
Surrogate
Recovery6
%
44.9
55.2
54.6
80.0
29.5
20.7
R-L
44.5
54.8
72.6
59.8
29.9
25.3
R-L
29.7
R-L
R-l
28.0
26.4
41.9
46.6
66.9
57.7
39.6
29.0
27.2
30.9
40.5
41.4
R-l
R-l
Concentration
(Recovery Corrected)
[ng/L]
7.70
8.85
6.81
46.9
11.4
6.64
3.72
9.50
9.15
16.6
24.4
5.31
42.8
292
217
7.92
18.0
8.26
73.1
5.10
39.9
67.0
46.0
27.4
38.5
A-12
-------
Table A-8. Continued.
ERL-D
Station - C
Battelle
ERL-D
Station - D
Battelle
ERL-D
Week of
Sample
Collection
1-a
2-b
3-c
4-d
4-d
1-C
1-H
2-D
2-N
3-L
4-I
4-I
1-a
2-b
3-c
3-c
4-d
1-A
1-H
1-B
2-D
2-M
3-F
3-L
4-K
4-K
1-a
2-b
2-b
3-c
4-d
Surrogate
Recovery1'
%
44.5
53.4
R-H
67.1
62.7
55.6
37.3
39.6
45.6
R-l
R-l
R-l
39.5
57.6
76.5
53.7
80.3
R-L
40.5
42.3
30.1
48.5
29.8
R-l
R-L
20.1
46.7
55.7
R-H
55.5
66.5
Concentration
(Recovery Corrected)
[ng/L]
11.6
20.4
60.1
61.8
11.1
13.7
33.4
28.3
4.52
11.1
1.33
1.28
3.89
4.27
21.0
51.4
30.2
3.82
230
8.79
14.5
0.896
43.9
A-13
-------
Table A-8. Continued.
Station E
Battetle
ERL^D
Week of
Sample
Collection
1-A
1-H
2-C
2-M
3-F
3-L
4-I
4-I
1-a
2-b
3-c
4-d
Surrogate
Recovery1'
%
R-L
39.1
35.9
27.5
33.9
25.5
R-l
R-l
45.0
R-P
59.2
63.3
Concentration
(Recovery Corrected)
[ng/L]
10.1
30.8
29.8
1.12
1.49
4.48
0.526
32.5
The letter following the week is the procedural blank done with the analysis
of the sample.
Surrogate: 1,2,4,5-13C8 TeCB, R-L: rejected, recovery <20%, R-H:
rejected, recovery >120%, R-l: rejected, incorrect data, R-P: rejected,
procedural error in sample preparation.
A-14
-------
Table A-9. Concentrations of Tetrachlorobutadiene #2 in Ambient Water
Samples.
Procedure Blanks
Battelle
ERL-D
Outfall
ERL-D
Station A
Battelle
Week of
Sample
Collection
A
B
C
D
E
F
G
H
1
J
K
L
M
N
a
b
c
d
1-a
1-a
2-b
3-c
4-d
1-A
1-G
1-B
2-D
2-M
3-E
3-N
4-K
4-K
Surrogate
Recovery6
%
R-L
46.4
33.6
29.7
39.4
51.9
45.7
34.2
29.1
R-L
55.4
53.9
40.6
37.6
88.2
106
84.3
93.0
39.9
39.7
40.5
52.3
53.7
33.6
29.3
20.9
28.7
29.1
21.1
31.7
R-L
22.7
Concentration
(Recovery Corrected)
[ng/L]
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.814
1.26
2.95
1.69
2.79
46.2
25.6
43.9
34.3
77.3
75.2
57.3
422
A-15
-------
Table A-9. Continued.
Week of
Sample
Collection
ERL-D
Station 1 - Composite
Battelle
ERL-D
Station 1 - Grab
Battelle
ERL-D
Station B
Battelle
1-a
2-b
3-c
4-d
1-A
2-C
3-E
1-a
2-b
3-c
4-d
1-B
1-B
2-E
2-N
3-F
3-L
4-J
4-J
1-a
2-b
3-c
4-d
1-C
1-H
2-D
2-M
3-E
3-N
4-J
4-J
Surrogate
Recovery1'
%
44.9
55.2
54.6
80.0
29.5
20.7
R-L
44.5
54.8
72.6
59.8
29.9
25.3
R-L
29.7
R-L
R-l
28.0
20.1
41.9
46.6
66.9
57.7
39.6
29.0
27.2
30.9
40.4
41.3
R-l
R-l
Concentration
(Recovery Corrected)
[ng/L]
16.6
24.4
14.7
181
28.5
27.3
6.85
21.8
17.5
37.8
53.9
10.1
105
628
570
15.9
41.8
18.5
254
13.2
78.1
113
113
61.3
74.5
A-16
-------
Table A-9. Continued.
ERL-D
Station C
Battelle
ERL-D
Station D
Battelle
ERL-D
Week of
Sample
Collection
1-a
2-b
3-c
4-d
4-d
1-C
1-H
2-D
2-N
3-L
4-I
4-I
1-a
2-b
3-c
3-c
4-d
1-A
1-H
1-B
2-D
2-M
3-F
3-L
4-K
4-K
1-a
2-b
2-b
3-c
4-d
Surrogate
Recovery1'
%
44.5
53.4
R-H
67.1
62.7
55.5
37.3
39.6
45.5
R-l
R-l
R-l
39.5
57.6
76.5
53.7
80.3
R-L
40.5
42.3
30.1
48.5
29.8
R-l
R-L
20.1
46.7
55.7
R-H
55.5
66.5
Concentration
(Recovery Corrected)
[ng/L]
24.2
44.9
202
205
30.8
28.3
80.7
72.5
11.90
33.50
5.15
5.50
13.0
31.2
45.8
117
62.3
10.9
708
18.7
34.0
4.36
224
A-17
-------
Table A-9. Continued.
Station E
Battelle
ERL-D
Week of
Sample
Collection
1-A
1-H
2-C
2-M
3-F
3-L
4-I
4-I
1-a
2-b
3-c
4-d
Surrogate
Recovery*1
%
R-L
39.1
35.9
27.5
33.9
25.5
R-l
n-i
45.0
R-P
59.2
63.3
Concentration
(Recovery Corrected)
[ng/L]
22.6
83.1
8.13
4.84
15.1
11.1
2.97
102
The letter following the week is the procedural blank done with the analysis
of the sample.
Surrogate: 1,2,4,5-13Ce TeCB, R-L: rejected, recovery <20%, R-H:
rejected, recovery >120%, R-l: rejected, incorrect data, R-P: rejected,
procedural error in sample preparation.
A-18
-------
Table A-10. Concentrations of Pentachlorobutadiene #1 in Ambient Water
Samples.
Procedure Blanks
Battelle
ERL-D
Outfall
ERL-D
Station A
Battelle
Week of
Sample
Collection
A
B
C
D
E
F
G
H
1
J
K
L
M
N
1-a
2-b
3-c
4-d
1-a
1-a
2-b
3-c
4-d
1-A
1-G
1-B
2-D
2-M
3-E
3-N
4-K
Surrogate
Recovery5
%
R-L
45.0
33.6
29.8
39.4
51.9
45.7
34.2
29.1
R-L
55.4
53.9
40.6
37.6
88.2
106
84.3
93.0
39.9
39.7
40.5
52.3
53.7
33.6
29.3
20.9
28.7
29.1
21.1
31.7
R-L
Concentration
(Recovery Corrected)
[ng/L]
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
8.23
5.92
8.44
5.38
8.50
247
202
277
204
515
605
381
A-19
-------
Table A-10. Continued.
ERL-D
Station 1 -
Battelle
ERL-D
Station 1 -
Battelle
ERL-D
Station B
Battelle
Week of
Sample
Collection
4-K
1-a
2-b
3-c
4-d
Composite
1-A
2-C
3-E
1-a
2-b
3-c
4-d
Grab
1-B
1-B
2-E
2-N
3-F
3-L
4-J
4-J
1-a
2-b
3-c
4-d
1-C
1-H
2-D
2-M
3-E
3-N
Surrogate
Recovery6
%
22.7
44.9
55.2
54.6
80.0
29.5
20.7
R-L
44.5
54.8
72.6
59.8
29.9
25.3
R-L
29.7
R-L
R-l
28.0
26.4
41.9
46.6
66.9
57.7
39.6
29.0
27.2
30.9
40.5
41.4
Concentration
(Recovery Corrected)
[ng/L]
2200
71.3
121
82.5
805
114
88.4
27.8
96.7
64.2
179
195
62.2
525
2770
3000
49.4
154
90.2
938
324
423
652
58.6
311
337
A-20
-------
Table A-10. Continued.
ERL-D
Station C
Battelle
ERL-D
Station D
Battelle
ERL-D
Week of
Sample
Collection
4-J
4-J
1-a
2-b
3-c
4-d
4-d
1-C
1-H
2-D
2-N
3-L
4-I
4-I
1-a
2-b
3-c
3-c
4-d
1-A
1-H
1-B
2-D
2-M
3-F
3-L
4-K
4-K
1-a
2-b
2-b
3-c
Surrogate
Recovery''
%
R-l
R-l
44.5
53.4
R-H
67.1
62.7
55.6
37.3
39.6
45.6
R-l
R-l
R-!
39.5
57.6
76.5
53.7
80.3
R-L
40.5
42.3
30.1
48.5
29.8
R-l
R-L
20.1
46.7
55.7
R-H
55.5
Concentration
(Recovery Corrected)
Ing/L]
77.5
199
739
859
149
156
181
144
32.7
45.0
0.00
0.00
16.9
211
245
364
285
0.603
1150
56.6
88.6
0.00
A-21
-------
Table A-10. Continued.
Week of
Sample
Collection
Surrogate Concentration
Recovery" (Recovery Corrected)
% [ng/L]
Station E
4-d
66.5
647
Battelle
ERL-D
1-A
1-H
2-C
2-M
3-F
3-L
4-I
4-I
1-a
2-b
3-c
4-d
R-L
39.1
35.9
27.5
33.9
25.5
R-l
R-l
45.0
R-P
59.2
63.3
165
222
240
0.00
1.18
26.9
0.00
369
The letter following the week is the procedural blank done with the analysis
of the sample.
Surrogate: 1,2,4,5-13Ca TeCB, R-L: rejected, recovery <20%, R-H:
rejected, recovery >120%, R-l: rejected, incorrect data, R-P: rejected,
procedural error in sample preparation.
A-22
-------
Tabla A-11. Concentrations of Pentachlorobutadiene #2 in Ambient Water Samples.
Procedure Blanks
Battelle
ERL-D
Outfall
ERL-D
Station A
Battelle
Week of"
Sample
Collection
A
B
C
0
E
F
G
H
I
J
K
L
M
N
a
b
c
d
1-a
1-a
2-b
3-c
4-d
1-A
1-G
1-B
2-D
2-M
3-E
3-N
4-K
4-K
Surrogate
Recovery1"
%
R-L
45.0
33.6
29.8
39.4
51.9
45.7
34.2
29.1
R-L
55.4
53.9
40.6
37.6
88.2
106
84.3
93.0
39.9
39.7
40.5
52.3
53.7
33.6
29.3
20.9
28.7
29.1
21.1
31.7
R-L
22.7
Concentration
(Recovery Corrected)
[ng/L]
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
3.57
3.20
6.35
2.99
2.72
72.5
58.6
81.1
59.6
132
175
109
837
A-23
-------
Table A-11. Continued.
Week of
Sample
Collection
ERL-D
Station 1 - Composite
Battelle
ERL-D
Station 1 - Grab
Battelle
ERL-D
Station B
Battelle
1-a
2-b
3-c
4-d
1-A
2-C
3-E
1-a
2-b
3-c
4-d
1-B
1-B
2-E
2-N
3-F
3-L
4-J
4-J
1-a
2-b
3-c
4-d
1-C
1-H
2-D
2-M
3-E
3-N
4-J
4-J
Surrogate
Recovery11
%
44.9
55.2
54.6
80.0
29.5
20.7
R-L
44.5
54.8
72,6
59.8
29.9
25.3
R-L
29.7
R-L
R-l
28.0
26.4
41.9
46.6
66.9
57.7
39.6
29.0
27.2
30.9
40.5
41.4
R-l
R-l
Concentration
(Recovery Corrected)
Ing/L]
20.5
29.3
20.6
223
37.7
31.0
7.88
28.2
23.3
41.5
72.6
21.3
177
1080
1100
17.0
43.2
24.0
282
90.0
115
207
21.3
109
127
A-24
-------
Table A-11. Continued.
ERL-D
Station C
Battelle
ERL-D
Station D
Battelle
ERL-D
Week of
Sample
Collection
1-a
2-b
3-c
4-d
4-d
1-C
1-H
2-D
2-N
3-L
4-I
4-I
1-a
2-b
3-c
3-c
4-d
1-A
1-H
1-B
2-D
2-M
3-F
3-L
4-K
4-K
1-a
2-b
2-b
3-c
4-d
Surrogate
Recovery11
%
44.5
53.4
R-H
67.1
62.7
55.6
37.3
39.6
45.6
R-l
R-l
R-l
39.5
57.6
76.5
53.7
80.3
R-L
40.5
42.3
30.1
48.5
29.8
R-l
R-L
20.1
46.7
55.7
R-H
55.5
66.5
Concentration
(Recovery Corrected)
[ng/L]
21.0
52.0
241
257
42.0
34.9
117
93.9
9.35
26.3
0.530
0.420
3.89
38.0
58.7
169
121
2.88
3170
15.8
36.0
0.00
43.9
A-25
-------
Table A-11. Continued.
Station E
Battelle
ERL-D
Week of
Sample
Collection
1-A
1-H
2-C
2-M
3-F
3-L
4-I
4-I
1-a
2-b
3-c
4-d
Surrogate
Recovery1'
%
R-L
39.1
35.9
27.5
33.9
25.5
R-l
R-l
45.0
R-P
59.2
63.3
Concentration
(Recovery Corrected)
[ng/L]
32.1
112
115
1.47
3.77
8.85
0.00
85.8
The letter following the week is the procedural blank done with the analysis of
the sample.
Surrogate: 1,2,4,5-13Ce TeCB, R-L: rejected, recovery < 20%, R-H: rejected,
recovery >120%, R-l: rejected, incorrect data, R-P: rejected, procedural error
in sample preparation.
A-26
-------
Table A-12. Concentrations of Hexachlorobutadiene in Ambient Water Samples.
Procedure Blanks
Battelle
ERL-D
Outfall
ERL-D
Station A
Battelle
Week of
Sample
Collection
A
B
C
D
E
F
G
H
I
J
K
L
M
N
a
b
c
d
1-a
1-a
2-b
3-c
4-d
1-A
1-G
1-B
2-D
2-M
3-E
3-N
4-K
4-K
Surrogate
Recovery6
%
R-L
45.0
33.6
29.8
39.4
51.9
45.7
34.2
29.1
R-L
55.4
53.9
40.6
37.6
88.2
106
84.3
93.0
39.9
39.7
40.5
52.3
53.7
33.6
29.3
20.9
28.7
29.1
21.1
31.7
R-L
22.7
Concentration
(Recovery Corrected)
[ng/L]
0.501
0.787
0.202
0.00
0.362
0.455
0.00
0.282
1.34
0.520
0.542
0.402
0.00
0.410
0.00
1.72
59.2
44.9
254
84.3
174
333
342
360
586
770
860
501
3740
A-27
-------
TableA-12. Continued.
Week of
Sample
Collection
ERL-D
Station 1 - Composite
Battelle
ERL-D
Station 1 - Grab
Battelle
ERL-D
Station B
Battelle
1-a
2-b
3-c
4-d
1-A
2-C
3-E
1-a
2-b
3-c
4-d
1-B
1-B
2-E
2-N
3-F
3-L
4-J
4-J
1-a
2-b
3-c
4-d
1-C
1-H
2-D
2-M
3-E
3-N
4-J
4-J
Surrogate
Recovery"
%
44.9
55.2
54.6
80.0
29.5
20.7
R-L
44.5
54.8
72.6
59.8
29.9
25.3
R-L
29.7
R-L
R-l
28.0
26.4
41.9
46.6
66.9
57.7
39.6
29.0
27.2
30.9
40.5
41.4
R-l
R-l
Concentration
(Recovery Corrected)
[ng/L]
521
494
433
4820
141
385
116
409
408
1540
304
100
688
4700
5550
302
674
499
4880
577
829
906
899
547
514
A-28
-------
Table A-12. Continued.
ERL-D
Station C
Battelle
ERL-D
Station D
Battelle
ERL-D
Week of
Sample
Collection
1-a
2-b
3-c
4-d
4-d
1-C
1-H
2-D
2-N
3-L
4-I
4-I
1-a
2-b
3-c
3-c
4-d
1-A
1-H
1-B
2-D
2-M
3-F
3-L
4-K
4-K
1-a
2-b
2-b
3-c
4-d
Surrogate
Recovery1"
%
44.5
53.4
R-H
67.1
62.7
55.6
37.3
39.6
45.6
R-l
R-l
R-l
39.5
57.6
76.5
53.7
80.3
R-L
40.5
42.3
30.1
48.5
29.8
R-l
R-L
20.1
46.7
55.7
R-H
55.5
66.5
Concentration
(Recovery Corrected)
[ng/L]
830
867
4360
5200
270
239
475
321
197
359
0.908
0.874
181
359
357
636
526
1.67
5520
377
525
1.10
3740
A-29
-------
Table A-12. Continued.
Station E
Battelle
ERL-D
Week of
Sample
Collection
1-A
1-H
2-C
2-M
3-F
3-L
4-I
4-I
1-a
2-b
3-c
4-d
Surrogate
Recovery*
%
R-L
39.1
35.9
27.5
33.9
25.5
R-l
R-l
45.0
R-P
59.2
63.3
Concentration
(Recovery Corrected)
[ng/L]
266
423
490
1.06
4.63
182
0.916
2740
The letter following the week is the procedural blank done with the analysis
of the sample.
Surrogate: 1,2,4,5-13C0 TeCB, R-L: rejected, recovery < 20%, R-H:
rejected, recovery >120%, R-l: rejected, incorrect data, R-P: rejected,
procedural error in sample preparation.
A 30
-------
Table A-13. Concentrations off 1,2,3-Trichlorobenzene in Ambient Water Samples.
Procedure Blanks
Battelle
ERL-D
Outfall
ERL-D
Station A
Batteile
Week of
Sample
Collection
A
B
C
D
E
F
G
H
I
J
K
L
M
N
a
b
c
d
1-a
1-a
2-b
3-c
4-d
1-A
1-G
1-B
2-D
2-M
3-E
3-N
4-K
4-K
Surrogate
Recovery6
%
R-L
45.0
33.6
29.8
39.4
51.9
45.7
34.2
29.1
R-L
55.4
53.9
40.6
37.6
88.2
106
84.3
93.0
39.9
39.7
40.5
52.3
53.7
33.6
29.3
20.9
28.7
29.1
21.1
31.7
R-L
22.7
Concentration
(Recovery Corrected)
[ng/L]
2.41
21.9
3.56
1.21
1.47
31.8
7.19
25.6
29.4
9.14
8.50
9.04
0.00
0.174
0.386
0.400
2.39
2.13
1.63
3.59
2.84
29.3
21.5
27.7
32.3
40.9
55.2
29.1
168
A-31
-------
Table A-13. Continued.
Week of
Sample
Collection
ERL-D
Station 1 • Composite
Battelle
ERL-D
Station 1 - Grab
Battelle
ERL-D
Station B
Battelle
1-a
2-b
3-c
4-d
1-A
2-C
3-E
1-a
2-b
3-c
4-d
1-B
1-B
2-E
2-N
3-F
3-L
4-J
4-J
1-a
2-b
3-c
4-d
1-C
1-H
2-D
2-M
3-E
3-N
4-J
4-J
Surrogate
Recovery1'
%
44.9
55.2
54.6
80.0
29.5
20.7
R-L
44.5
54.8
72.6
59.8
29.9
25.3
R-L
29.7
R-L
R-l
28.0
26.4
41.9
46.6
66.9
57.7
39.6
29.0
27.2
30.9
40.5
41.4
R-l
R-l
Concentration
(Recovery Corrected)
[ng/L]
22.5
12.5
23.4
157
33.1
57.3
12.0
11.6
29.0
43.1
26.1
13.5
48.4
193
210
22.9
20.7
25.9
229
51.2
45.0
48.1
51.9
30.1
39.2
A-32
-------
Table A-13. Continued.
ERL-D
Station C
Battelle
ERL-D
Station D
Battelle
ERL-D
Week of
Sample
Collection
1-a
2-b
3-c
4-d
4-d
1-C
1-H
2-D
2-N
3-L
4-I
4-I
1-a
2-b
3-c
3-c
4-d
1-A
1-H
1-B
2-D
2-M
3-F
3-L
4-K
4-K
1-a
2-b
2-b
3-c
4-d
Surrogate
Recover/
%
44.5
53.4
R-H
67.1
62.7
55.6
37.3
39.6
45.6
R-l
R-l
R-l
39.5
57.6
76.5
53.7
80.3
R-L
40.5
42.3
30.1
48.5
29.8
R-l
R-L
20.1
46.7
55.7
R-H
55.5
66.5
Concentration
(Recovery Corrected)
[ng/L]
27.3
26.5
192
197
68.4
21.3
57.6
37.1
14.8
16.0
5.99
6.40
13.5
27.1
21.1
41.0
38.6
7.76
225
21.5
16.5
4.27
152
A-33
-------
Table A-13. Continued.
Station E
Battelle
ERL-D
Week of
Sample
Collection
1-A
1-H
2-C
2-M
3-F
3-L
4-I
4-I
1-a
2-b
3-c
4-d
Surrogate
Recovery1'
%
R-L
39.1
35.9
27.5
33.9
25.5
R-l
R-l
45.0
R-P
59.2
63.3
Concentration
(Recovery Corrected)
[ng/L]
47.7
32.1
37.6
3.89
31.3
12.7
2.64
90.5
The letter following the week is the procedural blank done with the analysis
of the sample.
Surrogate: 1,2,4,5-13C0 TeCB, R-L: rejected, recovery <20%, R-H:
rejected, recovery >120%, R-l: rejected, incorrect data, R-P: rejected,
procedural error in sample preparation.
A-34
-------
Table A-14. Concentrations of 1,2,4-Trichlorobenzene in Ambient Water Samples.
Procedure Blanks
Battelle
ERL-D
Outfall
ERL-D
Station A
Battelle
Week of
Sample
Collection
A
B
C
D
E
F
G
H
1
J
K
L
M
N
a
b
c
d
1-a
1-a
2-b
3-c
4-d
1-A
1-G
1-B
2-D
2-M
3-E
3-N
4-K
4-K
Surrogate
Recovery*1
%
R-L
45.0
33.6
29.8
39.4
51.9
45.7
34.2
29.1
R-L
55.4
53.9
40.6
37.6
88.2
106
84.3
93.0
39.9
39.7
40.5
52.3
53.7
33.6
29.3
20.9
28.7
29.1
21.1
31.7
R-L
22.7
Concentration
(Recovery Corrected)
[ng/L]
4.26
3.66
1.55
1.76
1.55
7.17
2.92
6.08
7.69
3.40
4.00
4.24
1.30
0.415
0.00
0.00
11.0
9.75
16.5
15.0
13.2
116
87.3
117
139
191
218
138
872
A-35
-------
Table A-14. Continued.
Week of
Sample
Collection
ERL-D
Station 1 - Composite
Battelle
ERL-D
Station 1 - Grab
Battelle
ERL-D
Station B
Battelle
1-a
2-b
3-c
4-d
1-A
2-C
3-E
1-a
2-b
3-c
4-d
1-B
1-B
2-E
2-N
3-F
3-L
4-J
4-J
1-a
2-b
3-c
4-d
1-C
1-H
2-D
2-M
3-E
Surrogate
Recovery*1
%
44.9
55.2
54.6
80.0
29.5
20.7
R-L
44.5
54.8
72.6
59.8
29.9
25.3
R-L
29.7
R-L
R-l
28.0
26.4
41.9
46.6
66.9
57.7
39.6
29.0
27.2
30.9
40.5
Concentration
(Recovery Corrected)
[ng/L]
105
125
95.6
734
57.4
120
46.7
115
121
222
111
44.1
233
1050
1260
95.8
203
104
1080
152
210
259
258
153
A-36
-------
Table A-14. Continued.
ERL-D
Station C
Battelle
ERL-D
Station D
Battelle
Week of
Sample
Collection
3-N
4-J
4-J
1-a
2-b
3-c
4-d
4-d
1-C
1-H
2-D
2-N
3-L
4-I
4-I
1-a
2-b
3-c
3-c
4-d
1-A
1-H
1-B
2-D
2-M
3-F
3-L
4-K
4-K
Surrogate
Recovery1'
%
41.4
R-l
R-l
44.5
53.4
R-H
67.1
62.7
55.6
37.3
39.6
45.6
R-l
R-l
R-l
39.5
57.6
76.5
53.7
80.3
R-L
40.5
42.3
30.1
48.5
29.8
R-l
R-L
20.1
Concentration
(Recovery Corrected)
[ng/L]
170
139
216
330
1040
90.6
81.6
212
162
68.5
140
22.0
26.5
58.2
106
114
206
183
26.0
1460
A-37
-------
Table A-14. Continued.
-
ERL-D
Week of
Sample
Collection
1-a
2-b
2-b
3-c
4-d
Surrogate
Recover/
46.7
55.7
R-H
55.5
66.5
Concentration
(Recovery Corrected)
[ng/L]
105
158
15.3
821
Station E
Battelle
ERL-D
1-A
1-H
2-C
2-M
3-F
3-L
4-I
4-I
1-a
2-b
3-c
4-4
R-L
39.1
35.9
27.5
33.9
25.5
R-l
R-l
45.0
R-P
59.2
63.3
81.6
172
180
13.0
31.7
58.6
7.41
458
The fetter following the week is the procedural blank done with the analysis
of the sample.
Surrogate: 1.2,4,5-13Ce TeCB, R-L: rejected, recovery <20%, R-H:
rejected, recovery >120%, R-l: rejected, incorrect data, R-P: rejected,
procedural error in sample preparation.
A-38
-------
Table A-15. Concentrations of 1,2,4,5- and 1,2,3,5-Tetrachlorobenzene in
Ambient Water Samples.
Procedure Blanks
Battelle
,
ERL-D
Outfall
ERL-D
Statipn A
Battelle
Week of
Sample
Collection
A
B
C
D
E
F
G
H
I
J
K
L
M
N
a
b
c
d
1-a
1-a
2-b
3-c
4-d
1-A
1-G
1-B
2-D
2-M
3-E
3-N
4-K
Surrogate
Recovery5
%
R-L
45.0
33.6
29.8
39.4
51.9
45.7
34.2
29.1
R-L
55.4
53.9
40.6
37.6
88.2
106
84.3
93.0
39.9
39.7
40.5
52.3
53.7
33.6
29.3
20.9
28.7
29.1
21.1
31.7
R-L
Concentration
(Recovery Corrected)
[ng/L]
1.11
0.00
2.02
0.507
0.617
0.00
0.00
0.00
0.397
0.00
0.00
0.00
1.24
1.05
1.23
1.65
9.36
8.49
16.5
16.9
13.6
19.4
22.9
19.4
14.2
2.3
44.9
26.8
A-39
-------
TableA-15. Continued.
Week of
Sample
Collection
ERL-D
Station 1 - Composite
Battelle
ERL-D
Station 1 - Grab
Battelle
ERL-D
Station B
Battelle
4-K
1-a
2-b
3-c
4-d
1-A
2-C
3-E
1-a
2-b
3-c
4-d
1-B
1-B
2-E
2-N
3-F
3-L
4-J
4-J
1-a
2-b
3-c
4-d
1-C
1-H
2-D
2-M
3-E
3-N
Surrogate
Recoveryb
%
22.7
44.9
55.2
54.6
80.0
29.5
20.7
R-L
44.5
54.8
72.6
59.8
29.9
25.3
R-L
29.7
R-L
R-l
28.0
26.4
41.9
46.6
66.9
57.7
39.6
29.0
27.2
30.9
40.5
41.4
Concentration
(Recovery Corrected)
[ng/LJ
256
45.3
50.9
56.6
616
8.81
7.62
20.7
49.8
76.2
130
19.6
18.3
45.1
330
348
43.6
87.0
66.7
895
29.6
39.3
48.3
43.7
31.8
37.7
A-40
-------
Table A-15. Continued.
ERL-D
Station C
Battelle
ERL-D
Station D
Battelle
ERL-D
Week of
Sample
Collection
4-J
4-J
1-a
2-b
3-c
4-d
4-d
1-C
1-H
2-D
2-N
3-L
4-I
4-I
1-a
2-b
3-c
3-c
4-d
1-A
1-H
1-B
2-D
2-M
3-F
3-L
4-K
4-K
1-a
2-b
2-b
3-c
Surrogate
Recovery"
%
R-l
R-l
44.5
53.4
R-H
67.1
62.7
55.6
37.3
39.6
45.6
R-l
R-l
R-l
39.5
57.6
76.5
53.7
80.3
R-L
40.5
42.3
30.1
48.5
29.8
R-l
R-L
20.1
46.7
55.7
R-H
55.5
Concentration
(Recovery Corrected)
[ng/L]
66.2
99.0
858
805
17.7
17.7
44.7
36.1
38.9
82.7
25.0
27.5
42.3
20.8
23.5
44.2
34.9
9.72
349
48.7
84.8
19.0
A-41
-------
TableA-15. Continued.
Week of
Sample
Collection
Surrogate Concentration
Recovery" (Recovery Corrected)
% lng/L]
4-d
66.5
587
Station E
Battelle
ERL-D
1-A
1-H
2-C
2-M
3-F
3-L
4-I
4-I
1-a
2-b
3-c
4-d
R-L
39.1
35.9
27.5
33.9
25.5
R-l
R-l
45.0
R-P
59.2
63.3
18.9
32.5
37.0
7.02
11.3
33.3
13.4
362
The letter following the week is the procedural blank done with the analysis
of the sample.
Surrogate: 1,2,4,5-13Ce TeCB, R-L: rejected, recovery <20%, R-H:
rejected, recovery >120%, R-l: rejected, incorrect data, R-P: rejected,
procedural error in sample preparation.
A-42
-------
Table A-16. Concentrations of 1,2,3.4-Tetrachlorobenzene in Ambient Water
Samples.
Procedure Blanks
Battelle
ERL-D
Outfall
ERL-D
Station A
Battelle
Week of
Sample
Collection
A
B
C
D
E
F
G
H
I
J
K
L
M
N
a
b
c
d
1-a
1-a
2-b
3-c
4-d
1-A
1-G
1-B
2-D
2-M
3-E
3-N
4-K
Surrogate
Recovery1'
%
R-L
45.0
33.6
29.8
39.4
51.9
45.7
34.2
29.1
R-L
55.4
53.9
40.6
37.6
88.2
106
84.3
93.0
39.9
39.7
40.5
52.3
53.7
33.6
29.3
20.9
28.7
29.1
21.1
31.7
R-L
Concentration
(Recovery Corrected)
[ng/L]
0.00
0.00
0.00
0.00
0.347
2.37
0.00
2.27
2.46
0.928
0.00
1.33
0.00
0.00
0.00
0.00
4.74
3.15
5.94
10.8
6.55
25.9
33.5
26.9
15.2
3.16
69.6
40.4
A-43
-------
Table A-16. Continued.
Week of
Sample
Collection
ERL-D
Station 1 - Composite
Battelle
ERL-D
Station 1 - Grab
Battelle
ERL-D
Station B
Battelle
*
4-K
1-a
2-b
3-c
4-d
1-A
2-C
3-E
1-a
2-b
3-c
4-d
1-B
1-B
2-E
2-N
3-F
3-L
4-J
4-J
1-a
2-b
3-c
4-d
1-C
1-H
2-D
2-M
3-E
3-N
Surrogate
Recovery6
%
22.7
44.9
55.2
54.6
80.0
29.5
20.7
R-L
44.5
54.8
72.6
59.8
29.9
25.3
R-L
29.7
R-L
R-l
28.0
26.4
41.9
46.6
66.9
57.7
39.6
29.0
27.2
30.9
40.5
41.4
Concentration
(Recovery Corrected)
[ng/L]
349
26.6
16.0
39.1
345
5.76
12.7
10.7
35.9
41.9
83.1
27.7
28.1
1.14
458
496
23.9
56.2
41.9
462
35.0
46.0
67.2
61.0
44.1
50.9
A-44
-------
Table A-16. Continued.
ERL-D
Station C
Battelle
ERL-D
Station D
Battelle
ERL-D
Week of
Sample
Collection
4-J
4-J
1-a
2-b
3-c
4-d
4-d
1-C
1-H
2-D
2-N
3-L
4-I
4-I
1-a
2-b
3-c
3-c
4-d
1-A
1-H
1-B
2-D
2-M
3-F
3-L
4-K
4-K
1-a
2-b
2-b
3-c
Surrogate
Recovery6
%
R-l
R-l
44.5
53.4
R-H
67.1
62.7
55.6
37.3
39.6
45.5
R-l
R-l
R-l
39.5
57.6
76.5
53.7
80.3
R-L
40.5
42.3
30.1
48.5
29.8
R-l
R-L
20.1
46.7
55.7
R-H
55.5
Concentration
(Recovery Corrected)
Ing/L]
41.3
64.3
424
471
20.6
21.0
44.9
45.0
21.3
56.2
15.8
16.9
22.2
24.8
29.3
64.1
49.3
11.8
486
27.4
57.5
12.2
A-45
-------
TableA-16. Continued.
Week of
Sample
Collection
Surrogate
Recovery*1
Concentration
(Recovery Corrected)
[ng/L]
Station E
4-d
66.5
330
Battelle
ERL-D
1-A
1-H
2-C
2-M
3-F
3-L
4-I
4-I
1-a
2-b
3-c
4-d
R-L
39.1
35.9
27.5
33.9
25.5
R-l
R-l
45.0
R-P
59.2
63.3
25.8
41.7
50.3
8.79
14.6
17.5
8.45
197
The letter following the week is the procedural blank done with the analysis
of the sample.
Surrogate: 1,2,4,5-13Ce TeCB, R-L: rejected, recovery <20%, R-H:
rejected, recovery >120%, R-l: rejected, incorrect data, R-P: rejected,
procedural error in sample preparation.
A-46
-------
Table A-17. Concentrations of Pentachlorobenzene in Ambient Water Samples.
Procedure Blanks
Battelie
ERL-D
Outfall
ERL-D
Station A
Battelie
Week of
Sample
Collection
A
B
C
D
E
F
G
H
I
J
K
L
M
N
a
b
c
d
1-a
1-a
2-b
3-c
4-d
1-A
1-G
1-B
2-D
2-M
3-E
3-N
4-K
4-K
Surrogate
Recover/
%
R-L
49.5
39.8
32.1
44.3
52.7
49.2
40.3
31.8
R-L
60.1
56.8
42.2
44.1
94.2
104
79.4
120
48.3
56.3
44.2
48.9
74.6
36.0
37.8
R-L
37.6
48.8
35.8
39.9
27.1
R-L
Concentration
(Recovery Corrected)
[ng/L]
0.866
1.01
0.00
0.00
1.11
1.48
0.466
1.42
1.59
0.794
0.756
0.714
0.00
0.00
0.00
0.00
17.8
13.6
54.7
32.1
27.7
41.2
46.1
49.6
46.0
54.0
40.8
916
A-47
-------
TableA-17. Continued.
Week of
Sample
Collection
ERL-D
Station 1 - Composite
Battelle
ERL-D
Station 1 - Grab
Battelle
ERL-D
Station B
Battelle
1-a
2-b
3-c
4-d
1-A
2-C
3-E
1-a
2-b
3-c
4-d
1-B
1-B
2-E
2-N
3-F
3-L
4-J
4-J
1-a
2-b
3-c
4-d
1-C
1-H
2-D
2-M
3-E
3-N
4-J
4-J
Surrogate
Recovery5
%
53.9
44.5
53.4
87.7
33.0
26.1
22.7
54.9
53.4
62.1
72.9
33.5
50.0
R-L
35.4
32.2
R-l
34.3
31.2
43.6
45.5
58.2
43.8
41.9
43.2
36.4
38.3
42.8
50.1
R-l
R-l
Concentration
(Recovery Corrected)
[ng/L]
34.7
62.1
47.7
795
19.5
48.7
118
17.1
56.2
76.9
148
37.7
35.0
75.4
37.6
938
1060
34.2
81.9
60.3
1490
72.0
69.4
75.4
72.0
57.7
57.4
A-48
-------
Table A-17. Continued.
ERL-P
Station C
Battelle
ERL-D
Station D
Battelle
ERL-D
Week of
Sample
Collection
1-a
2-b
3-c
4-d
4-d
1-C
1-H
2-D
2-N
3-L
4-I
4-I
1-a
2-b
3-c
3-c
4-d
1-A
1-H
1-B
2-D
2-M
3-F
3-L
4-K
4-K
1-a
2-b
2-b
3-c
4-d
Surrogate
Recovery**
%
56.1
52.9
R-H
60.7
61.5
60.4
43.2
52.9
51.0
R-l
R-l
R-l
47.0
57.1
70.1
50.8
94.6
21.8
47.0
50.2
38.9
51.2
36.3
R-l
30.9
34.7
58.3
54.2
R-H
54.3
68.2
Concentration
(Recovery Corrected)
[ng/L]
78.9
84.8
1180
1200
29.3
24.8
65.6
54.4
23.2
65.1
21.7
24.4
23.3
52.8
33.0
39.0
70.8
67.7
15.3
552
542
29.9
72.3
17.8
540
A-49
-------
Table A-17. Continued.
Station E
Battelle
ERL-D
Week of
Sample
Collection
1-A
1-H
2-C
2-M
3-F
3-L
4-I
4-I
1
2
3
4
Surrogate
Recovery*1
%
R-L
37.8
39.3
37.4
39.0
29.6
R-l
R-l
52.7
R-P
56.4
75.7
Concentration
(Recovery Corrected)
[ng/L]
41.0
59.8
54.0
12.7
17.2
22.9
12.9
287
The letter following the week is the procedural blank done with the analysis
of the sample.
Surrogate: 1,2,4,5-13Ce HCB, R-L: rejected, recovery <20%, R-H:
rejected, recovery >120%, R-l: rejected, incorrect data, R-P: rejected,
procedural error in sample preparation.
A-50
-------
Table A-18. Concentrations of Hexachlorobenzene in Ambient Water Samples.
Procedure Blanks
Battelle
ERL-D
Outfall
ERL-D
Station A
Battelle
Week of
Sample
Collection
A
B
C
D
E
F
G
H
I
J
K
L
M
N
a
b
c
d
1-a
1-a
2-b
3-c
4-d
1-A
1-G
2-D
2-M
3-E
3-N
4-K
4-K
Surrogate
Recover/
%
R-L
49.5
39.8
32.1
44.3
52.7
49.2
40.3
31.8
R-L
60.1
56.8
42.2
44.1
94.2
104
79.4
120
48.3
56.3
44.2
48.9
74.6
36.0
37.8
37.6
48.8
35.8
39.9
27.1
R-L
Concentration
(Recovery Corrected)
[ng/L]
1.08
1.18
0.125
0.00
1.25
1.23
0.492
1.21
1.32
1.55
0.887
0.695
0.00
0.204
0.252
0.00
49.0
40.5
189
88.1
189
39.6
58.9
42.1
30.0
49.1
39.0
618
A-51
-------
Table A-18. Continued.
Week of
Sample
Collection
ERL-D
Station 1 - Composite
Battelle
ERL-D
Station 1 - Grab
Battelle
ERL-D
Station B
Battelle
1-a
2-b
3-c
4-d
1-A
2-C
3-E
1-a
2-b
3-c
4-d
1-B
1-B
2-E
2-N
3-F
3-L
4-J
4-J
1-a
2-b
3-c
4-d
1-C
1-H
2-D
2-M
3-E
3-N
4-J
4-J
Surrogate
Recover/
%
53.9
44.5
53.4
87.7
33.0
26.1
22.7
54.9
53.4
62.1
72.9
33.5
50.0
R-L
35.4
32.2
R-l
34.3
31.2
43.6
45.5
58.2
43.8
41.9
43.2
36.4
38.3
42.8
50.1
R-l
R-l
Concentration
(Recovery Corrected)
[ng/L]
29.0
25.3
39.7
861
19.9
32.9
86.7
14.3
28.3
67.6
138
30.8
33.1
34.3
57.8
805
970
24.8
34.9
55.4
2240
145
110
36.8
33.0
44.6
44.0
A-52
-------
Table A-18. Continued.
ERL-D
Station C
Battelle
ERL-D
-
Station D
Battelle
ERL-D
Week of
Sample
Collection
1-a
2-b
3-c
4-d
4-d
1-C
1-H
2-D
2-N
3-L
4-I
4-I
1-a
2-b
3-c
3-c
4-d
1-A
1-H
1-B
2-D
2-M
3-F
3-L
4-K
4-K
1-a
2-b
2-b
3-c
4-d
Surrogate
Recovery1'
%
56.1
52.9
R-H
60.7
61.5
60.4
43.2
52.9
51.0
R-l
R-l
R-l
47.0
57.1
70.1
50.8
94.6
21.8
47.0
50.2
38.9
51.2
36.3
R-l
30.9
34.7
58.3
54.2
R-H
54.3
68.2
Concentration
(Recovery Corrected)
[ng/L]
129
39.9
1210
1720
24.9
16.0
27.3
25.6
14.9
26.8
17.5
17.3
8.37
37.8
26.4
25.6
33.5
31.8
13.8
269
284
24.6
35.5
15.6
242
A-53
-------
Table A-18. Continued.
Station E
Battelle
ERL-D
Week of
Sample
Collection
1-A
1-H
2-C
2-M
3-F
3-L
4-1
4-1
1-»
2-b
3-c
4-d
Surrogate
Recover/
%
R-L
37.8
39.3
37.4
39.0
29.6
R-l
R-l
52.7
R-P
56.4
75.7
Concentration
(Recovery Corrected)
[ng/L]
34.5
27.1
26.8
11.4
20.1
22.5
11.4
122
The letter following the week is the procedural blank done with the analysis
of the sample.
Surrogate: 1,2,4,5-13Ce HCB, R-L: rejected, recovery <20%, R-H:
rejected, recovery >120%, R-l: rejected, incorrect data, R-P: rejected,
procedural error in sample preparation.
A-54
-------
Table A-19. Dissolved Chemical Concentrations for Ambient Composite Water Samples
from Station 1.
Hexachloroethane 13CrHCE Recovery (%) HCE
Week 1 18.0 55.5
Week 2 26.5 159
Week 3 18.9 125
Blank 57.9 4.46
Tetrachlorobutadiene 11 13C,-TeCB Recovery 1%) TeCBD *1 (na/L)
Week 1 18.7 7.51
Week 2 26.7 20.2
Week 3 20.3 25.6
Blank 45.7 0
Tetrachlorobutadiene #2 13Cg-TeCB Recovery {%) TeCBD #2 (nq/L>
Week 1 18.7 30.9
Week 2 26.7 55.7
Week3 20.3 81.1
Blank 45.7 0
Pentachlorobutadiene #1 13Cf-TeCB Recovery (%) PeCBD #1 (nq/Ll
Week 1 18.7 99.9
Week 2 26.7 286
Week 3 20.3 246
Blank 45.7 0
Pentachlorobutadiene #2 13Cfl-TeCB Recovery _(%> PeCBD #2 (ng/L)
Week 1 18.7 35.0
Week 2 26.7 94.7
Week3 20.3 101
- Blank 45.7 0
Hexachlorobutadiene 13C9-TeCB Recovery (%i HCBD
-------
Table A-19. Continued.
Trichlorobenzene. 1.2.3- 13C«-TeCB Recovery (%) TrCB. 1.2.3- (no/L)
Week 1 18.7 12.9
Week 2 26.7 26.8
Week 3 20.3 73.7
Blank 45.7 31.8
Trichlorobenzene. 1.2.4- 13Cff-TeCB Recovery (%J TrCB. 1.2.4- (ng/L)
Week 1 18.7 54.8
Week 2 26.7 136
Week 3 20.3 1 50
Blank 45.7 7.17
Tetrachlorobenzene Mix 13C.-TeCB Recovery <%) TeCB Mix (no/L>
Week 1 18.7 8.61
Week 2 26.7 27.0
WeekS 20.3 31.6
Blank 45.7 0
Tetrachlorobenzene. 1.2.3.4- 13Cff-TeCB Recovery (%) TeCB. 1.2.3.4- (na/L)
Week 1 18.7 14.4
Week 2 26.7 41.3
WeekS 20.3 48.1
Blank 45.7 2.37
Pentachlorobenzene 13Cg-HCB Recovery <%) PeCB (no/Li
Week 1 25.6 14.0
Week 2 37.0 45.1
Week 3 20.6 54.5
Blank 49.2 1.48
Hexachlorobenzene 13C9-HCB Recovery (%1 HCB (no/U
Week 1 25.6 10.6
Week 2 37.0 19.4
WeekS 20.6 34.1
Blank 49.2 1.23
A-56
-------
Table A-20. Particulate Chemical Concentrations for Ambient Composite Water Samples
from Station 1.
Hexachloroethane 13CrHCE Recovery (%> HCE (ng/L)
Week 1 30.4 3.10
Week 2 10.3 1.90
Week 3 41.4 2.48
Week 4 9.80 2.68
Blank 48.0 1.91
Tetrachlorobutadiene #1 13Cff-TeCB Recovery (%) TeCBD 11 (ng/L)
Week 1 51.8 0
Week 2 12.0 0
Week 3 52.9 0
Week 4 40.6 0
Blank 49.5 0
Tetrachlorobutadiene *2 13C^-TeCB Recovery (%) TeCBD #2 (ng/l,!
Week 1 51.8 0
Week 2 12.0 0
Week 3 52.9 0
Week 4 40.6 0
Blank 49.5 0
Pentachlorobutadiene *1 ^C^-TeCS Recovery (%) PeCBD #1 (ng/U
Week 1 51.8 0
Week 2 12.0 0
Week 3 52.9 1.65
Week 4 40.6 0.580
Blank 49.5 0
Pentachlorobutadiene #2 13Cf-TeCB Recovery (%1 PeCBD #2 (ng/L)
Week 1 51.8 0
Week 2 12.0 0
Week 3 52.9 0.507
Week 4 40.6 0
Blank 49.5 0
A-57
-------
Table A-20. Continued.
fjexachlorobutadiene
Week 1
Week 2
Week 3
Week 4
Blank
Trichlorobenzene. 1.2.3-
Week 1
Week 2
Week 3
Week 4
Blank
T_ific.h|orobenzejie. 1.2.4-
Week 1
Week 2
Week 3
Week 4
Blank
Tetrachlorobenzene Mix
- Week 1
Week 2
Week 3
Week 4
Blank
Tetrachlorobenzene. 1.2.3.4-
Week 1
Week 2
Week 3
Week 4
Blank
13C.-TeCB Recovery (%)
51.8
12.0
52.9
40.6
49.5
13C?-TeCB Recovery (%)
51.8
12.0
52.9
40.6
49.5
13C.-TeCB Recovery (%)
51.8
12.0
52.9
40.6
49.5
13Cfl-TeCB Recovery (%)
51.8
12.0
52.9
40.6
49.5
13Cfl-TeCB Recovery (%)
51.8
12.0
52.9
40.6
49.5
HCBD (no/IJ
7.33
7.08
16.2
60.1
0.275
TrCB. 1.2.3- (no/Ll
6.25
3.60
5.85
4.24
7.14
TrCB. 1.2.4- (no/L)
1.87
1.24
2.31
3.72
1.70
TeCB Mix (na/L>
0.541
0
0,454
0.837
0
TeCB. 1.2.3.4- (na/L>
0.386
0
1.04
1.21
0
A-58
-------
Table A-20. Continued.
Pentachlorobenzene 13C.-HCB Recovery (%) PeCB (no/L)
Week 1 62.1 2.38
Week 2 17.1 2.48
Week 3 64.3 6.13
Week 4 53.7 24.9
Blank 60.0 0.632
Hexachlorobenzene 13Cff-HCB Recovery {%) HCB (na/Ll
Week 1 62.1 7.45
Week 2 17.1 6.70
Week3 64.3 . 14.0
Week 4 53.7 60.7
Blank 60.0 5.05
A-59
-------
Table A-21. POC and DOC for Ambient Water Samples.
POC:
Station
POC. //a/filter1
A
B
C
D
E
1 Grab
1 Composite
Weekl
580
440
510
360
610
620
710
Week 2
400
370
350
340
400
330
330
Week 3
400
270
330
390
380
300
mean of 9 blank samples = 184//g/filter (std. dev. = 31 //g/filter)
sample size = 100 ml
DOC:
Station
DOC. mo/L
Week 4
360
410
460
380
400
420
400
Week 1
8.6
7.2
11.0
7.8
7.7
60
363
Week 2
81
94
50
81
73
82
39
Week 3
78
84
77
76
78
33
67
Week 4s
A
B
C
D
E
1 Grab
1 Composite
mean of 9 blank samples = 1.4 mg/L (std. dev. = 0.5 mg/L)
2 Samples were not analyzed because holding time of samples were exceeded.
A-60
-------
Table A-22. DOC, POC, and TOC Values for Ambient Water Samples.
Week Station
1 A
B
C
D
E
1 Grab
1 Composite
2 A
B
C
D
E
1 Grab
1 Composite
3 A
B
C
D
E
1 Grab
1 Composite
4 A
B
C
D
E
1 Grab
1 Composite
Organic
DOC1
7.2
5.8
9.6
6.4
6.3
59
362
80
93
49
80
72
81
38
77
83
76
75
77
32
66
Carbon. mg/L
POC2
4.0
2.6
3.3
1.8
4.3
4.4
5.3
2.2
1.9
1.7
1.6
2.2
1.5
1.5
2.2
0.9
1.5
2.1
2.0
1.2
1.8
2.3
2.8
2.0
2.2
2.4
2.2
TOC1
7.1
11.6
7.5
6.2
12.6
68
408
81
101
61
82
84
89
68
1 Corrected for background (1.4 mg/L)
2 Corrected for background (184/yg/filter)
A-61
-------
Table A-23. Percentages of Daily Composite Versus Daily Grab Samples in the
Seven Day Composite Water Samples from Station 1.
Sampling Date % Composite % Grab
Week 1
Sept. 12/13 100 0
Sept. 13/14 33 66
Sept. 14/15 33 66
Sept. 15/16 33 66
Sept. 16/17 50 50
Sept. 17/18 0 100
Sept. 18/19 100 0
Week 2
Sept. 19/20 33 66
Sept. 20/21 100 0
Sept. 21/22 100 0
Sept. 22/23 0 100
Sept. 23/24 33 66
Sept. 24/25 0 100
Sept. 25/26 0 100
Week 3
Sept. 26/27 100 0
Sept. 27/28 0 100
Sept. 28/29 100 0
Sept. 29/30 12 88
Sept. 30/Oct. 1 0 100
Oct. 1/2 100 0
Oct. 2/3 100 0
Week 4
Oct. 3/4 100 0
Oct. 4/5 100 0
Oct. 5/6 100 0
Oct. 6/7 100 0
Oct. 7/8 100 0
Oct. 8/9 100 0
A-62
-------
Table A-24. Organic Carbon Content for Sediment Samples.
Station
A
1
B
C
D
E
Percent Organic Carbon1
7.19
3.69
9.12
2.99
4.14
5.31
1 Percent organic carbon = total carbon - carbonate carbon
A-63
-------
Table A-25. Particle Size Distribution for Sediments.
1
t 1
Particle Size Distribution (% <2 mm)
Sand (mm)
VCS
Station 2-1
A 0.8
1 5.0
B 0.7
C 0.4
D 0.2
E 0.4
V = Very
C = Coarse
S = Sand
M = Medium
F = Fine
SI = Silt
C = Clay
T = Total
CS MS
1-0.5 0.5-0.25
1.1 2.2
3.3 2.6
1.1 1.6
0.4 0.3
0.9 1.2
0.3 0.6
FS
0.25-0.1
16.9
21.6
23.8
10.1
23.2
15.3
VFS
0.1-0.05
32.5
1.6
17.7
17.4
18.2
17.4
TS
2-0.05
53.5
34.1
44.9
28.6
43.7
34.0
CSI
50-20
14.7
44.5
1B.O
16.8
11.6
19.0
Silt torn)
MSI
20-5
8.5
5.0
8.0
11.3
B.5
9.8
FSI
5-2
2.9
1.9
3.B
6.9
4.6
6.0
TSI
50-2
26.1
51.4
29.B
35.0
24.7
34.8
CC
2-0.2
12.0
9.9
11.7
20.8
14.3
14.5
Clav dim)
FC
<0.2
8.4
4.6
13.6
15.6
17.3
16.7
TC
<2
20.4
14.5
25.3
36.4
31.6
31.2
Text.
Class
SCL
SIL
L
CL
CL
CL
A-64
-------
Tabla A-26. Tiisua C
itlng Information.
For aach coapoalta saapla, th« *at waigttt (g) of tha organiui and tha fiald laapla
package fro» which that organ I a» MM randomly choMn ara listad.
>ja hataroelituB
Station A
Haaua •••• 9
n>
Avaraga naaa
std Oav.
Coaf.var.
Station 1 Coapoaita 1
poaita 1
•0009
•0011
•0009
•0011
-0012
•0011
•0012
-0012
•0009
-0010
•0010
-0012
Co
2.7
2.4
2.1
3.4
14.2
1.8
5.4
6.9
10.8
4.5
9.0
5.8
71.0
12
5.9
4.0
67.2
•poaita 2
•0009
•0009
•0011
•0012
•0012
•0012
•0009
•0012
•0011
•0012
•0010
•0009
Co
4.1
7.9
2.2
5.0
9.3
4.7
2.5
5.8
2.0
2.9
5.4
1.7
53.5
12
4.5
2.4
53.9
apoalti
•0010
•0011
-0009
-0011
-0010
-0011
•0011
•0010
•0010
-0012
•0011
•0011
•Oil
•011
011
009
009
009
009
•009
009
009
009
009
7.1
4.6
1.5
9.3
2.1
6.5
1.7
1.9
3.3
2.0
2.4
2.1
Tiisua MM g
n>
Avaraga naia
Std Oav.
Coaf.var.
Station I C
U.5
12
3.7
2.6
69.7
ita 1
CoapMitc 2
ita 3
1.9
1.4
2.1
2.5
5.3
2.5
4.7
6.0
3.4
6.1
4.7
2.3
42.9
12
3.6
1.7
47.3
Ita 4
•0012
•0009
•0011
•0009
•0010
•0010
•0010
•0009
•0012
•0011
•0012
•0010
14.0
6.7
2.2
.4
.7
.0
.7
.7
1 .6
.0
.1
.6
78.1
12
6.5
4.2
65.0
fta 4
Goapoaita 5
Ita 6
•0017 ;
-014 t
•014
•0020
•013
-0018
•0016
•0020 ;
•0020 1
•0018 «
•0020 :
-0017 <
(.4
..7
.0
.6
.6
.2
.2
'.0
1.2
M
1.4
>.1
•0017
•0020
•0013
•0013
•0020
•0017
-0020
-0013
•0018
•0018
-0017
•0020
8.4
2.2
3.6
1.9
3.4
3.8
5.8
2.4
4.7
4.8
3.S
4.3
-0013
-0019
•0014
•0017
-0019
•0020
•0020
•0017
-0014
•0019
-0017
•0017
5.1
9.3
4.4
4.2
5.6
10.6
4.8
6.2
5.7
10.3
3.0
7.4
•0017
-0014
•0014
•0017
•0014
•0013
•0013
•0014
•0013
•0013
•0018
•0013
4.7
1.9
10.4
7.0
11.5
1.8
2.4
3.5
2.6
2.4
6.8
3.4
•0013
•0017
•0013
•0018
•0014
•0018
•0014
•0014
•0014
•0018
•0018
•0014
4.4
4.0
5.4
6.4
14.1
14.2
3.3
8.4
1.5
12.4
13. t
3.4
•0018
•0018
•0018
•0018
-0013
•0014
•0014
•0014
•0014
-0014
•0014
-0014
6.6
4.5
0.7
1.S
0.6
3.6
J.I
2.0
1.2
1.3
1.1
1.6
-------
Tiitu* Mat §
n»
A*«r>M MM
Std 0*v.
Co*f.¥if.
Station C c
It* i
TlMUt MM
r»
Av*raM MM
ltd 0«v.
Cotf.Vir.
Stitton D C
-------
n*
Avtragt man
Std Dev.
Coef.var.
Callinectes tapidue
Station A Coeposite 1
12
3.0
O.S
2A.2
Whol* MM (g) include* shell and soft body aitheut legs.
it* 2
it* 3
Whole
Soft
Whole
Soft
Whole
Soft
r issue MSS g
n>
Average MSS
Std Oev.
Ceef.ver.
Station 1 Co
Tissue MSS g
n«
Average MSS
Std Oev.
Coef.var.
Station I ca
Tissue MSS g
n«
Average MSS
Std Oev.
Coef.var.
Station C Ca
Tissue mess g
n»
Average maa
Std Dtv.
Coef.var.
Station 0 Ca
•0001
-0002
•0003
•petite 1
•0004
•0002
-0005
•posit* 1
-012
•0009
•0010
•poeite 1
•oooa
•0009
•0009
•poaite 1
•OOU
-0004
94.6
123.7
110.3
328.6
3
109.S
14. A
13.3
Whole
184. 5
14o.O
240.8
S71.3
3
190.4
47.7
23. 0
Whole
179.0
147.8
114.2
441.0
3
147.0
32.4
22.0
Whole
24?.*
244. 1
20S.*
702.3
3
234.2
22.1
9.5
Whole
229.8
171.2
22.4
33.S
36.0
91.9
3
30.6
7.2
23.6
Soft
91. ft
63. 2
96.4
233.4
3
84.3
16.8
19.9
Soft
92.3
36.6
42.6
191.7
3
63.9
23.7
40.3
Soft
97.
93.
8*.
275.
91.
6.
7.2
Soft
89. 8
76.2
•0001
-0007
-0003
Coaposite
•0003
•0003
•0003
Coeposit*
•012
•Oil
•Oil
Cospoait*
•0001
•0007
-0007
Cosamit*
•0003
-0015
125.7
101.6
202.0
429.3
3
143.1
52.4
36.6
2
Whole
130.3
91.2
94.7
316.4
3
105.5
21.8
20.6
2
whole
171.6
181.1
208.6
561.3
3
187.1
19.2
10.3
2
whole
56.6
64.8
48.2
169.6
3
56.3
8.3
14.7
2
whole
118.3
99.8
34.3 -0002 138.
62.7 -0002° 110.
40.8 -0001 103.
137.8 352.
3
43.9 117.
' 14.9 18.
32.4 13.
Coapoaite 3
Soft Whole
44.4 -0004 71.3
39.1 -0003 82.2
50.8 -0002 68.6
134.3 222.1
3 3
44.1 74.0
3.9 7.2
13.1 9.7
Soft
70.4
76.6
74.1
221.1
3
73.7
3.1
4.2
Soft
20.
26.
20.
67.
22.
3.
13.7
Soft
54.5
39.6
50.6
38.9
27.7
117.2
3
3«.1
11.3
29.3
loft
34.2
28.3
28.1
92.1
3
30.9
4.6
14.8
Caapeilte 4
Whole
Soft
86.0
67.8
56.4
210.2
3
70.1
14.9
21.3
28.3
24.5
18.7
71.7
3
23.9
4.9
20.6
-------
Titty* •
n*
Av«rag«
Std Dtv.
Coaf.Var
Station
TUtua •
n*
Av«rag«
Std 0«v.
Coaf.Var
-0018
•tt g
Mil
E Caapoait* 1
•0003
-0002
-000*
•U 0
•Ml
.
145.7
5U.7
3
182.2
43.1
23.7
y*oia
228.4
232.8
2U.6
70J.8
3
235.3
8.4
3.6
83.1
249.1
3
83.0
6.8
8.2
Ca
Scft
98.9
90.4
108.4
297.9
3
99.3
9.1
9.2
-0015 91.8
309.
103.
13.
13.
•*M
AVVCA^f) MBftS
Std D«v.
Co«*.Var.
Station 0
Tis»u* man
n*
-0006
-0006
•0006
g
Coapoalt* 1
-0006
-0006
-0006
g
CoapMit* 1
-0010
•0010
-0010
g
Avvrag* mail
Std Otv.
Co*f .Var.
Station E
Conpoait* 1
•0011
-0011
•0011
28.6
28.3
21.7
78.6
3
26.2
3.9..*
14.9
21.0
20.8
18.2
S9.9
3
20.0
1.6
7.8
(A) Cc
24.1
20.f
25.2
70.9
3
23.6
2.4
10.2
20.7
19.3
22. S
it* 2 (I) CoapMitt 3 (C)
•0019
20.7
27.7
27.9
76.3
3
25.4
4.1
16.1
•0009
•0009
•0019
24.7
20.
19.
64.
21.
2.
13.
-------
Titsua IMS* 9
n*
Avtrigt «•••
Std Otv.
Co«f.V«r.
20.
1,
7,
Irrvoorttit p*trenu§
Station C CoMmit* 1 (A) C
>
-------
Table A-27. Hexachloroethane Tissue Concentrations for Fundulus heteroclitus.
Station
A
Comp #1
Comp #2
Comp #3
Comp #4
Comp #4
1
Comp #1
B
Comp #1
Comp #1
Comp #2
Comp #3
Comp #3
Comp #4
Comp #5
Comp #6
C
Comp #1
Comp #1
D
Comp #1
Comp #2
Comp #2
Comp #2
Comp #3
E
Comp #1
Laboratory8
Bat E
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
ERL-D 5/17
Bat E
Bat E
Bat E
ERL-D 5/15
Bat E
ERL-D 5/17
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/15
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
Lipid
%
0.975
1.01
1.42
0.612
1.42
1.24
0.794
1.37
0.615
1.58
1.54
0.954
2.04
0.825
0.994
2.18
0.930
1.03
0.988
1.30
1.31
13CrHCE
Recovery11
%
42.6
16.3C
55.1
16.7
17.1
18.7
20.8
20.4
R-L
39.0
36.7
23.8
20.1
45.8
18.0
20.3
24.8
39.2
38.0
20.1
19.1
20.4
Concentration
(Recovery
Corrected)
[ng/g]
78.0
55.6
82.6
69.6
65.8
154
107
91.1
128
119
202
112
103
102
90.0
104
20.8
22.3
29.4
9.97
94.9
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
523
396
384
827
336
925
995
489
563
520
943
868
343
912
665
325
145
142
203
40.6
533
A-70
-------
Tiliu* MM
n*
Avtr»9« MI
std otv.
Cotf.Var.
Stition E
T(l«u> MM
nm
Av«rag« Mai
Std Dtv.
Coaf.Var.
NicropoMn i
Station 8
TIlMJt MM
n*
Avtrtg* MI
Std D«v.
Cotf.Var.
Station C
Tiliua MM
n*
Avtrag* MI
Std Otv.
Cotf .var.
Station 0
Tisiu* MM
nm
Average Ma
Std Otv.
Coef.Var.
Station E
-0018
9
I
Coapoett*
•0003
-0002
•0004
9
l
jvlulm
Coapoaftt
•0006
•0006
•0006
9
l
Coapoait*
•0006
•0006
-0006
9
l
COMDOSit*
-0010
-0010
•0010
9
i
COMDOSit*
•0011
-0011
•0011
145.7
5*6.7
3
182.2
43.1
23.7
1
Who It
228.4
232.
244.
703.
235.
8.4
3.6
1
28.6
28.3
21.7
78.6
3
26.2
3.9..*
14.9
1
21.0
20.8
18.2
59.9
3
20.0
1.6
7.8
1 («> Co
24.
20.
29.
70.
23.
2.4
10.2
1
20.7
19.3
22.5
83.1 -0015 91.8 36.9
249.1 309. 131.0
3 . 3
83.0 103. 43.7
6.8 13. 9.5
8.2 13. 21.7
CoapMit* 2 Coopoaitt 3
Scft urtolt Soft Uholt
98.9 -0002 193.0 74.1 -0004 154.5
90.4 -0005 216.9 90.4 -0001 ' 169.6
108.6 -0001 195.7 65.3 -0003 164.7
297.9 605.6 229.8 488.8
3 33 3
99.3 201.9 76.6 162.9
9.1 13.1 12.7 7.7
9.2 6.5 16.6 4.7
•potitt 2 (8) Coapoait* 3 (C)
•0009 20.7 -0009 24.7
•0009 27.7 -0009 20.
•0019 27.9 -0019 19.
76.3 64.
3
2S.4 21.
4.1 2.
16.1 13.
Soft
64.7
54.8
59.4
178.9
3
59.6
5.0
8.3
-------
Average ness
Std Oev.
Coef.var.
12
3.0
O.I
26.2
CaUineetn sapidua
Station A Coapoefte 1
Whole
(g) Includes shell end toft body without legs.
Ite 2
Whole
Soft
Whole
Coaposlta 3
Soft whole
Soft
Tissue MI
0"
Average •
Std Dev.
Coef.var.
Station 1
Tissue MI
0"
Average m
Std Oev.
Coef.var.
Station 1
Tissue MI
n*
Average M
Std Dev.
Coef.var.
Station C
•0001
•0002
•0003
is g
MS
Coapoaite 1
•0004
-0002
-0005
ta g
ISS
Coapoalte 1
-012
•0009
•0010
w g
•as
Canoe ite 1
•OOM
•0009
•0009
Tissue mass g
n»
Average mass
Std Dev.
Coef.var.
Stirion D
Conpoeite 1
•0014
-0004
94.6
123.7
110.3
328.
109.
14.
13.
Whole
184.5
146.0
240.8
571.3
3
190.4
47.7
23.0
Whole
179.0
147.8
114.2
441.0
3
147.0
32.4
22.0
Whole
249.6
244.1
208.8
702.5
3
234.2
22.1
9.5
Whole
229.8
171.2
22.4
33.
36.
91.
30.
7.
23.
Soft
91.8
65.2
96.4
253.4
3
84.5
16.8
19.9
Soft
92.5
56.6
42.6
191.7
3
63.9
23.7
40.3
Soft
97.
93.
84.
273.
91.
6.
7.
Soft
89.8
76.2
•0001
-0007
•0003
Coapoaite
•0003
•0005
•0003
Coapoaite
•012
•Oil
•Oil
Coapoalta
•0001
•0007
•0007
Coapoaite
-0005
•0015
123.7
101.6
202.0
429.3
3
143.1
52.4
36.6
2
Whole
130.3
91.2
94.7
316.4
3
105.5
21.8
20.6
2
Whole
171.6
181.1
208.6
561.3
3
187.1
19.2
10.3
2
Whole
56.6
64.8
48.2
169.6
3
56.5
8.3
14.7
2
Whole
118.3
99.8
34.3
62.7
40.8
137.8
3
45.9
14.9
32.4
Co
Soft
44.4
39.1
50.8
134.3
3
44.8
5.9
13.1
Soft
70.4
76.6
74.1
221.1
3
73.7
3.1
4.2
Soft
20.9
26.6
20.1
67.6
3
22.3
3.3
13.7
Soft
54.5
39.6
•0002 138.0
•0002' 110.6
-0001 103.6
352.2
3
117.4
18.2
15.5
epoalte 3
Whole
•0004 71 .3
•0003 82.2
•0002 68.6
222.1
3
74.0
7.2
9.7
50.6
38.9
27.7
117.2
3
39.1
11.3
29.3
Coapoaite 4
Soft whole Soft
36.2 -0004 86.0 28.
28.5 -0006 67.8 24.
28.1 -0006 36.4 18.
92.1 210.2 71.
3 3
30.9 70.1 23.
4.6 14.9 4.
14.8 21.3 20.
-------
Table A-27. Hexachloroethane Tissue Concentrations for Fundulus heteroclitus.
Station
A
Comp #1
Comp #2
Comp #3
Comp #4
Comp #4
1
Comp #1
B
Comp #1
Comp #1
Comp #2
Comp #3
Comp #3
Comp #4
Comp #5
Comp #6
C
Comp #1
Comp #1
D
Comp #1
Comp #2
Comp #2
Comp #2
Comp #3
E
Comp #1
Laboratory*
Bat E
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/1 7
ERL-D 5/17
Bat E
Bat E
Bat E
ERL-D 5/15
Bat E
ERL-D 5/17
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/15
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
Lipid
%
0.975
1.01
1.42
0.612
1.42
1.24
0.794
1.37
0.615
1.58
1.54
0.954
2.04
0.825
0.994
2.18
0.930
1.03
0.988
1.30
1.31
13CrHCE
Recovery1"
%
42.6
16.3e
55.1
16.7
17.1
18.7
20.8
20.4
R-L
39.0
36.7
23.8
20.1
45.8
18.0
20.3
24.8
39.2
38.0
20.1
19.1
20.4
Concentration
(Recovery
Corrected)
[ng/g]
78.0
55.6
82.6
69.6
65.8
154
107
91.1
128
119
202
112
103
102
90.0
104
20.8
22.3
29.4
9.97
94.9
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
523
396
384
827
336
925
995
489
563
520
943
868
343
912
665
325
145
142
203
40.6
533
A-70
-------
Tiiiua MMt |
n*
Std Otv.
Coaf.Vaf.
62,
20,
1,
7.
irtvoortU patron*
Station C
itt 1 (A) Coapoaitt 2 (I) Coapoaitt 3 (C) C
fta ft (0) C
it* S (I) C
ftt 6
Tliaua MM 9
n*
Avaraga a*aa
Std Oav.
Coaf.Var.
Ca
TillUt MMt f
n«
Avarafla Mtt
Std D*v.
Coaf.Var.
Station D Ca
Tii*ua «•«* g
n»
Avaraga MS*
Std Orv.
Coaf.Var.
•0011
•000ft
•0011
0005
•0002
•pocita 6
•0004
-0005
•0002
•001ft
-001ft
^Mita 1
•0020
•0020
•0020
•0011
•0011
Station E CM^asit* 1
rissut (tan 9
n«
Avarag* mail
Std Dtv.
Cocf.var.
-0010
-0010
•000?
•0010
•0010
8.4
12.1
11.1
12.2
1ft. 1
sa.s
!
11.7
2.0
17.4
(C)
12.2
12. •
11.9
12.6
13.S
44.9
S
13.0
Q.7
S.ft
(A)
11.2
10.6
6.5
10.2
8.5
ftr.o
5
9.4
l.f
20.4
(A)
10. •
12.4
10.7
9.2
Ift.ft
57.5
5
11.5
2.0
17.1
•0009
•0014
•0005
•0011
•000ft
C<«posita 2
•0011
-0011
•0011
•0011
-0011
CONpoafta 2
•OOW
•0009
•0009
-0010
•0007
H.8
10.*
13.6
10.3
15.1
6ft. ft
5
12.9
2.3
17.6
(1)
9.0
11.9
1.2
1.2
8.3
45. S
S
9.1
1.6
17.3
(•)
13.5
13.8
13.1
12.0
11.0
63.4
S
12.7
1.2
9.1
•000ft
•0005
•000ft
•0005 •
•001ft
CoMpoaita 3
-0009
-0010
-0010
•0009
•0009
13.i
10.3
15.6
13.3
11.1
64.8
S
13.0
2.0
15.7
(C) C«
9.1
10.5
8.7
11.7
12.3
52.2
S
10.4
1.6
15.2
-0005
-0002
•oou
•001ft
•001ft
«
•poalta ft
•0007
•000?
•0010
•0007
•0009
13.1 -001ft
11.7 -0014
17.1 -0014
17.4 -0005
14.8 -0004
74.7
S
15.3
2.5
16.0
(0)
13.4
10.6
9.0
10.4
11.3
54.7
5
10.9
1.6
u.a
13.2 -0014 11.5
9.7 -0011 i.S
12.7 -0004 11.1
12.7 -0011 14.1
11.2 -0004 11.1
39.5 56.3
5 5
11.9 11. J
1.4 2.0
12.0 17.6
-------
n«
Avtng* MM
Std D«v.
Coaf.Var.
Callinactaa
Station A
TiSMja MM*
n>
Avaraga MM
Std Dav.
Coaf.Var.
Station 1
Ha*** MM*
r»
Avaraga MM
Std Dav.
Coaf.Var.
Station I
TilMW MM
n«
Avaraga MB*
Std Oav.
Coaf.Var.
Station C
TiMua MM
n»
Avaraga MM
Std D«v.
Coaf.Var.
Station 0
MpidUB
Coapoaita 1
•0001
•0002
•0003
9
Coapoaita 1
•0004
-0002
•0005
g
Coapoaita 1
•012
•0009
•0010
9
Coapoaita 1
-0008
•0009
•0009
9
Coapoaita 1
•OOU
•0004
12
3.0
o.a
24.2
Uhola MI
Uhola
94.6
123.7
110.3
328.6
3
109. 5
14.6
13.3
Uhola
184. 5
144.0
240.8
571.3
3
190.4
47.7
2S.O
Uhola
179.0
147.8
114.2
441.0
3
147.0
32.4
22.0
Uhola
249.4
2U.1
208.8
702:5
3
234.2
22.1
9.5
Uhola
229.8
171.2
Soft
22.4
33.
36.
91.
30.
7.
23.
Soft
91.8
65.2
96.4
253.4
3
84.5
16.8
19.9
Soft
92.5
56.6
42.6
191.7
3
63.9
2S.7
40.3
soft
97.5
93.3
84.4
279.4
3
91.8
6.6
7.2
Soft
89.8
76.2
w (g) Includa* shall and aoft body without lag*.
Coapeaita
•0001
-0007
•0003
Coapoalt*
-0003
-0005
•0003
CoMoait*
•012
•011
•011
Coapoaita
•0001
•0007
•0007
Conpoaita
•0005
•0015
2
Uhola
125. 7
101.6
202.0
429.3
3
143.1
52.4
36.6
2
Uhola
130.5
91.2
94.7
316.4
3
105.5
21.8
20.6
2
Uhola
171.6
181.1
208.6
561.3
3
187.1
19.2
10.3
2
Uhola
56.
64.
48.
169.
56.
8.
14.7
2
Uhola
118.3
99.8
Coapoaita
Soft
34.3 -0002
62.7 -0002'
40.8 -0001
137.8
3
45.9
' 14.9
32.4
Coapoaita
Soft
44.4 -0004
39.1 -0003
50.8 -0002
134.3
3
44.8
5.9
13.1
Soft
70.4
76.6
74.1
221.1
3
73.7
3.1
4.2
Soft
20.
26.
20.
67.
22.
3.
15.7
Soft
54.5
39.6
3
Uhola
138.0
110.6
103.6
352.2
3
117.4
18.2
13.3
3
Uhola
71.3
82.2
68.6
222.1
3
74.0
7.2
9.7
Soft
50.6
38.9
27.7
117.2
3
39.1
11.3
29.3
Soft
34.2
28.5
28.1
92.8
3
30.9
4.4
14.8
Coapoalta 4
Uhola
Soft
•0004
84.0
47.8
54.4
210.2
3
70.1
14.9
21.3
28.5
24.5
18.7
71.7
3
23.9
4.9
20.6
-------
Table A-28. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13C6-TeCB (Recovery 7.6% Lipid
Lipid Recovery6 Corrected) Content)
Laboratory" % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/1 5
ERL-D 5/17
ERL-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
29.6
44.0
0.00d
0.00d
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-l: rejected, recovery <15%, R-H: rejected, recovery >120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-73
-------
Table A-29. Tetrachlorobutadiene #2 Tissue Concentrations for Fundulus heteroclitus.
Station "
A
Comp #1
Comp #2
Comp #3
Comp #4
Comp #4
1
Comp #1
B
Comp #1
Comp #1
Comp #2
Comp #3
Comp #3
Comp #4
Comp #5
Comp #6
C
Comp #1
Comp #1
D
Comp #1
Comp #2
Comp #2
Comp #2
Comp #3
E
Comp #1
Laboratory*
Bat E
ERL-D5/17
BatE
ERL-D5/15
ERL-D5/15
ERL-D 5/15
ERL-D 5/1 7
ERL-D 5/1 7
ERL-D 5/1 7
Bat E
BatE
BatE
ERL-D 5/15
BatE
ERL-D 5/1 7
ERL-D 5/1 7
Bat E
ERL-D 5/1 5
ERL-D 5/1 5
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
Lipid
%
0.975
1.01
1.42
0.612
1.42
1.24
0.794
1.37
0.615
1.58
1.54
0.954
2.04
0.825
0.994
2.18
0.930
1.03
0.988
1.30
1.31
13Ce-TeCB
Recovery*
%
30.7
18.1C
30.8
16.6
18.4
17.7
24.1
21.2
R-L
19.2
30.4
15.4
19.3
9.5
18.5
21.1
22.7
45.5
40.9
19.8
20.2
20.1
Concentration
(Recovery
Corrected)
[ng/g]
22.4
11.9
24.1
16.3
20.3
33.6
15.8
18.2
40.5
28.7
58.0
23.1
41.2
17.3
13.9
20.6
2.94
3.07
5.18
2.56
15.1
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
175
89.5
129
202
109
206
151
101
195
138
286
184
153
159
106
71.8
24.0
22.7
39.8
15.0
87.6
A-74
-------
Table A-27. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13C,-HCE (Recovery 7.6% Lipid
Lipid Recovery6 Corrected) Content)
Laboratory' % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/1 5
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
29.3
42.4
0.00d
0.00"
58.8
23.3
62.7
64.1
51.1
59.1
41.2
R-L
37.5
31.4
45.5
46.9
52.8
4.01
3.63
4.12
0.00
3.21
2.91
3.38
3.01
11.9
8.41
9.50
12.7
11.1
11.5
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery <15%, R-H: rejected, recovery >120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-71
-------
Table A-28. Tetrachlorobutadiene #1 Tissue Concentrations for Fundulus heteroclitus.
Station
A
Comp #1
Comp #2
Comp #3
Comp #4
Comp #4
1
Comp #1
B
Comp #1
Comp #1
Comp #2
Comp #3
Comp #3
Comp #4
Comp #5
Comp #6
C
Comp #1
Comp #1
D
Comp #1
Comp #2
Comp #2
Comp #2
Comp #3
E
Comp #1
Laboratory'
Bat E
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
ERL-D 5/17
Bat E
Bat E
Bat E
ERL-D 5/1 5
Bat E
ERL-D 5/17
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/15
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
Lipid
%
0.975
1.01
1.42
0.612
1.42
1.24
0.794
1.37
0.615
1.58
1.54
0.954
2.04
0.825
0.994
2.18
0.930
1.03
0.988
1.30
1.31
13C8-TeCB
Recovery11
%
30.7
18. 1C
30.8
16.6
18.4
17.7
24.1
21.2
R-L
19.2
30.4
15.4
19.3
29.5
18.5
21.1
22.7
45.5
40.9
19.8
20.2
20.1
Concentration
(Recovery
Corrected)
[ng/g]
5.71
1.44
8.12
2.60
2.43
5.05
2.90
3.28
16.4
8.58
22.5
2.73
14.6
3.03
2.56
10.9
0.00
0.00
0.709
0.00
2.29
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
44.5
10.8
43.5
32.3
13.0
31.0
27.8
18.2
78.9
41.3
111
21.7
54.4
27.9
19.6
38.0
0.00
0.00
5.45
0.00
13.3
A-72
-------
TiflLM Mil 9
n»
Avtngt Mil
Std 0«v.
Cotf.Vtr.
20.
1.
7,
ir*voorti» patroout
Stition C Cawwit* 1 (A) d
itt 2 (I) Ccapoaitt 3 (C) 0
Itt 4 (D)
Itt 9 (E) C
Itt 6 (F)
TfMUt MM fl
nm
Avtrtg* MM
Std 0«v.
Cotf.vtr.
Ca
Tiiiut MII g
Avtrtgt MII
Std Otv.
Cotf.vtr.
Stttion 0 Ca
Tiiiut M«I g
r\»
Avtrtgt Mil
Std Otv.
Cotf.vtr.
Station E Ca
Tiiiut Mil g
n*
Avtrtgt M*I
Std Otv.
Cotf .vtr.
-0011
-0004
-0011
-0005
-0002
notltt
•0004
-0009
-0002
•OOU
•0014
notltt
-0020
-0020
-0020
-0011
•0011
notttt
-0010
-0010
•0007
•0010
-0010
8.
12.
11.
12.
14.
98.
11.
2.
17.
6 (G)
12.
12.
13.
12.
13.
64.
13.
0.
5.
1 (A)
11.
10.
6.
10.
8.
47.
9.
1.
20.
1 (A)
10.
12.
10.
9.
14.
57.
11.
2.
17.
*
3
1
2
1
3
9
7
0
4
2
0
7
4
2
A
9
2
9
0
9
4
9
4
8
4
7
2
4
5
5
5
0
1
-0009
-0014
-0009
•0011
-0004
COBpMltt 2
•0011
-0011
-0011
-0011
-0011
Coapotttt 2
-0009
-0009
-0009
-0010
•0007
14.
10.
13.
10.
19.
64.
12.
2.
17.
(•)
9.
11.
8.
8.
8.
45.
9.
1.
17.
(!)
13.
13.
13.
12.
11.
63.
12.
1.
9
0
9
2
2
3
9
9
1
6
3
9
8
1
0
0
4
5
7
2
1
-0004
•0009
•0004
•0009
•0014
Co^ocict 3
•0009
-0010
•0010
•0009
-0009
13.
10.
19.
13.
11.
64.
13.0
2.0
19.7
(C) Co
9.1
10.9
8.7
11.7
12.3
52.2
5
10.4
1.6
15.2
-0009 19.
-0002 11.
-OOU 17.
-OOU 17.
-OOU 14.
76.
19.
2.
16.
•VOtUt 4 (D)
•0007 13
•0009 10
-0010 9
-0007 10
•0009 11
54
10
1
14
1 -OOU
7 -0014
8 -0014
4 -0009
8 -0004
7
9
3
9
0
.4
.6
.0
.4
.3
.7
5
.9
.6
.8
13.2 -0014 11.5
9.7 -0011 8.5
12.7 -0004 11.1
12.7 -0011 U.I
11.2 -0004 11.1
99.9 56.3
9 5
11.9 11. J
1.4 2.0
12.0 17.6
-------
Table A-27. Hexachloroethane Tissue Concentrations for Fundulus heteroclitus.
Station
A
Comp #1
Comp #2
Comp #3
Comp #4
Comp #4
1
Comp #1
B
Comp #1
Comp #1
Comp #2
Comp #3
Comp #3
Comp #4
Comp #5
Comp #6
C
Comp #1
Comp #1
D
Comp #1
Comp #2
Comp #2
Comp #2
Comp #3
E
Comp #1
Laboratory"
Bat E
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
ERL-D 5/17
Bat E
Bat E
Bat E
ERL-D 5/15
Bat E
ERL-D 5/17
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/15
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
Lipid
%
0.975
1.01
1.42
0.612
1.42
1.24
0.794
1.37
0.615
1.58
1.54
0.954
2.04
0.825
0.994
2.18
0.930
1.03
0.988
1.30
1.31
13C,-HCE
Recovery1"
%
42.6
16.3C
55.1
16.7
17.1
18.7
20.8
20.4
R-L
39.0
36.7
23.8
20.1
45.8
18.0
20.3
24.8
39.2
38.0
20.1
19.1
20.4
Concentration
(Recovery
Corrected)
lng/g]
78.0
55.6
82.6
69.6
65.8
154
107
91.1
128
119
202
112
103
102
90.0
104
20.8
22.3
29.4
9.97
94.9
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
523
396
384
827
336
925
995
489
563
520
943
868
343
912
665
325
145
142
203
40.6
533
A-70
-------
Table A-27. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Lipid
Laboratory" %
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/1 5
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
Concentration
Concentration (Blank Corrected,
13C,-HCE (Recovery 7.6% Lipid
Recovery6 Corrected) Content)
% [ng/g] [ng/g]
29.3
42.4
0.00d
0.00d
58.8
23.3
62.7
64.1
51.1
59.1
41.2
R-L
37.5
31.4
45.5
46.9
52.8
4.01
3.63
4.12
0.00
3.21
2.91
3.38
3.01
11.9
8.41
9.50
12.7
11.1
11.5
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery <15%, R-H: rejected, recovery >120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-71
-------
Table A-28. Tetrachlorobutadiene #1 Tissue Concentrations for Fundulus heteroclitus.
Station
A
Comp #1
Comp #2
Comp #3
Comp #4
Comp #4
1
Comp #1
B
Comp #1
Comp #1
Comp #2
Comp #3
Comp #3
Comp #4
Comp #5
Comp #6
C
Comp #1
Comp #1
D
Comp #1
Comp #2
Comp #2
Comp #2
Comp #3
E
Comp #1
Laboratory*
Bat E
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
ERL-D 5/17
Bat E
Bat E
Bat E
ERL-D 5/1 5
Bat E
ERL-D 5/17
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/15
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
Lipid
%
0.975
1.01
1.42
0.612
1.42
1.24
0.794
1.37
0.615
1.58
1.54
0.954
2.04
0.825
0.994
2.18
0.930
1.03
0.988
1.30
1.31
Concentration
Concentration (Blank Corrected,
13Cfl-TeCB (Recovery 7.6% Lipid
Recovery11 Corrected) Content)
% [ng/g] [ng/g]
30.7
18.1°
30.8
16.6
18.4
17.7
24.1
21.2
R-L
19.2
30.4
15.4
19.3
29.5
18.5
21.1
22.7
45.5
40.9
19.8
20.2
20.1
5.71
1.44
8.12
2.60
2.43
5.05
2.90
3.28
16.4
8.58
22.5
2.73
14.6
3.03
2.56
10.9
0.00
0.00
0.709
0.00
2.29
44.5
10.8
43.5
32.3
13.0
31.0
27.8
18.2
78.9
41.3
111
21.7
54.4
27.9
19.6
38.0
0.00
0.00
5.45
0.00
13.3
A-72
-------
Table A-28. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13C6-TeCB (Recovery 7.6% Lipid
Lipid Recovery6 Corrected) Content)
Laboratory' % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1 /24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
Bat A
Bat B
Bat C
BatD
Bat E
BatF
Bat G
29.6
44.0
0.00d
0.00d
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-l: rejected, recovery < 15%, R-H: rejected, recovery > 120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-73
-------
Table A-29. Tetrachlorobutadiene #2 Tissue Concentrations for Fundulus heteroclitus.
Station "
A
Comp #1
Comp #2
Comp #3
Comp #4
Comp #4
1
Comp #1
B
Comp #1
Comp #1
Comp #2
Comp #3
Comp #3
Comp #4
Comp #5
Comp #6
C
Comp #1
Comp #1
D
Comp #1
Comp #2
Comp #2
Comp #2
Comp #3
E
Comp #1
Laboratory*
BatE
ERL-D5/17
BatE
ERL-D 5/15
ERL-D 5/15
ERL-D 5/1 5
ERL-D 5/17
ERL-D 5/1 7
ERL-D 5/17
Bat E
BatE
BatE
ERL-D 5/1 5
BatE
ERL-D 5/17
ERL-D 5/17
BatE
ERL-D 5/15
ERL-D 5/1 5
ERL-D 4/23
ERL-D 5/1 5
ERL-D 5/1 5
Lipid
%
0.975
1.01
1.42
0.612
1.42
1.24
0.794
1.37
0.615
1.58
1.54
0.954
2.04
0.825
0.994
2.18
0.930
1.03
0.988
1.30
1.31
13Ca-TeCB
Recovery6
%
30.7
18.1°
30.8
16.6
18.4
17.7
24.1
21.2
R-L
19.2
30.4
15.4
19.3
9.5
18.5
21.1
22.7
45.5
40.9
19.8
20.2
20.1
Concentration
(Recovery
Corrected)
[ng/g]
22.4
11.9
24.1
16.3
20.3
33.6
15.8
18.2
40.5
28.7
58.0
23.1
41.2
17.3
13.9
20.6
2.94
3.07
5.18
2.56
15.1
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
175
89.5
129
202
109
206
151
101
195
138
286
184
153
159
106
71.8
24.0
22.7
39.8
15.0
87.6
A-74
-------
Table A-29. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Lipid
Laboratory" %
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/1 5
ERL-D 5/1 5
ERL-D 5/1 7
ERL-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
BatF
Bat G
Concentration
Concentration (Blank Corrected,
13C8-TeCB (Recovery 7.6% Lipid
Recoveryb Corrected) Content)
% [ng/g] [ng/g]
29.6
44.0
0.00d
0.00"
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery <15%, R-H: rejected, recovery >120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-75
-------
Table A-30. Pentachlorobutadiene #1 Tissue Concentrations for Fundulus heteroclitus.
Station
A
Comp #1
Comp #2
Comp #3
Comp #4
Comp #4
1
Comp #1
B
Comp #1
Comp #1
Comp #2
Comp #3
Comp #3
Comp #4
Comp #5
Comp #6
C
Comp #1
Comp #1
D
Comp #1
Comp #2
Comp #2
Comp #2
Comp #3
E
Comp #1
Laboratory"
Bat E
ERL-D5/17
Bat E
ERL-D5/15
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
ERL-D 5/17
Bat E
Bat E
BatE
ERL-D 5/15
BatE
ERL-D 5/17
ERL-D 5/17
BatE
ERL-D 5/15
ERL-D 5/15
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
Lip id
%
0.975
1.01
1.42
0.612
1.42
1.24
0.794
1.37
0.615
1.58
1.54
0.954
2.04
0.825
0.994
2.18
0.930
1.03
0.988
1.30
1.31
13C8-TeCB
Recovery6
%
30.7
18. 1C
30.8
16.6
18.4
17.7
24.1
21.2
R-L
19.2
30.4
15.4
19.3
29.5
18.5
21.1
22.7
45.5
40.9
19.8
20.2
20.1
Concentration
(Recovery
Corrected)
[ng/g]
380
92.5
110
100
86.3
270
136
146
656
460
1230
138
730
112
101
463
17.8
16.3
21.5
4.24
74.2
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
2960
696
587
1242
462
1655
1302
810
3154
2211
6069
1099
2718
1032
772
1613
145
120
165
24.8
430
A-76
-------
Table A-30. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13Ce-TeCB (Recovery 7.6% Lipid
Lipid Recovery" Corrected) Content)
Laboratory" % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/1 5
ERL-D 5/17
ERL-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
BatF
Bat G
29.6
44.0
0.00d
0.00d
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
2.19
0.00
0.00
0.00
0.00
0.00
0.00
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery <15%, R-H: rejected, recovery >120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-77
-------
Table A-31. Pentachlorobutadiene #2 Tissue Concentrations for Fundulus heteroclitus.
Station
A
Comp #1
Comp #2
Comp #3
Comp #4
Comp #4
1
Comp #1
B
Comp #1
Comp #1
Comp #2
Comp #3
Comp #3
Comp #4
Comp #5
Comp #6
C
Comp #1
Comp #1
D
Comp #1
Comp #2
Comp #2
Comp #2
Comp #3
CT5-E
Comp #1
Laboratory"
Bat E
ERL-D 5/17
Bat E
ERL-D 5/1 5
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
ERL-D 5/17
Bat E
Bat E
Bat E
ERL-D 5/15
Bat E
ERL-D 5/17
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/15
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
Lipid
%
0.975
1.01
1.42
0.612
1.42
1.24
0.794
1.37
0.615
1.58
1.54
0.954
2.04
0.825
0.994
2.18
0.930
1.03
0.988
1.30
1.31
13C8-TeCB
Recovery6
%
30.7
18.1°
30.8
16.6
18.4
17.7
24.1
21.2
R-L
19.2
30.4
15.4
19.3
29.5
18.5
21.1
22.7
45.5
40.9
19.8
20.2
20.1
Concentration
(Recovery
Corrected)
Ing/g]
97.0
17.1
0.00
21.2
18.5
58.7
25.7
31.3
184
139
294
29.3
174
24.3
24.3
121
4.42
5.00
5.32
2.05
17.2
Concentration
(Blank Corrected,
7.6% Lipid
Content)
Ing/g]
755
129
0.00
263
99.0
360
246
174
884
668
1450
233
648
224
186
421
36.1
36.9
40.9
12.0
100
A-78
-------
Table A-31. Continued.
Station -
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank .
Concentration
Concentration (Blank Corrected,
13Ca-TeCB (Recovery 7.6% Lipid
Lipid Recovery11 Corrected) Content)
Laboratory* % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
Bat A
Bat B
BatC
Bat D
BatE
Bat F
Bat G
29.6
44.0
0.00d
0.00d
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.948
0.00
0.00
0.00
0.00
0.00
0.00
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery <15%, R-H: rejected, recovery >120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A 79
-------
Table A-32. Hexachlorobutadiene Tissue Concentrations for Fundulus heteroclitus.
Station
A
Comp #1
Comp #2
Comp #3
Comp #4
Comp #4
1
Comp #1
B
Comp #1
Comp #1
Comp #2
Comp #3
Comp #3
Comp #4
Comp #5
Comp #6
C
Comp f 1
Comp #1
D
Comp #1
Comp #2
Cornp #2
Comp #2
Comp #3
E
Comp #1
Laboratory"
Bat E
ERL-D i/17
Bat E
ERL-D 5/1 5
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
ERL-D 5/17
Bat E
Bat E
Bat E
ERL-D 5/1 5
Bat E
ERL-D 5/17
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/1 5
ERL-D 4/23
ERL-D 5/1 5
ERL-D 5/15
Lipid
%
0.975
1.01
1.42
0.612
1.42
1.24
0.794
1.37
0.615
1.58
1.54
0.954
2.04
0.825
0.994
2.18
0.930
1.03
0.988
1.30
1.31
13Ce-TeCB
Recovery1*
%
30.7
18. V
30.8
16.6
18.4
17.7
24.1
21.2
R-L
19.2
30.4
15.4
19.3
29.5
18.5
21.1
22.7
45.5
40.9
19.8
20.2
20.1
Concentration
(Recovery
Corrected)
[ng/g]
2510
3960
2990
5990
4920
11400
7130
8320
5840
4270
6290
7940
3890
8540
7530
3270
1410
1540
1760
395
5890
Concentration
(Blank Corrected,
7.6% Lipid
Content)
Ing/g]
19538
29742
15984
74293
26292
69825
68175
46113
28074
20522
31024
63194
14479
78603
57516
11388
11461
11308
13481
2266
34128
A-80
-------
Table A-32. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13C8-TeCB (Recovery 7.6% Lipid
Lipid Recovery6 Corrected) Content)
Laboratory" % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/1 7
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
29.6
44.0
0.00d
0.00d
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
5.04
2.81
4.93
9.90
7.46
7.28
11.2
11.2
11.5
1.50
2.11
0.00
6.30
2.23
0.840
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery < 15%, R-H: rejected, recovery > 120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-81
-------
Table A-33. 1,2,3-Trichlorobenzene Tissue Concentrations for Fundulus heteroclitus.
Station
A
Comp #1
Comp #2
Comp #3
Comp #4
Comp #4
1
Comp #1
B
Comp #1
Comp #1
Comp #2
Comp #3
Comp #3
Comp #4
Comp #5
Comp #6
C
Comp #1
Comp #1
D
Comp #1
Comp #2
Comp #2
Comp #2
Comp #3
E
Comp #1
Laboratory"
Bat E
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
ERL-D 5/17
Bat E
Bat E
Bat E
ERL-D 5/15
Bat E
ERL-D 5/17
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/15
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
Lipid
%
0.975
1.01
1.42
0.612
1.42
1.24
0.794
1.37
0.615
1.58
1.54
0.954
2.04
0.825
0.994
2.18
0.930
1.03
0.988
1.30
1.31
13Ca-TeCB
Recovery11
%
30.7
18. 1C
30.8
16.6
18.4
17.7
24.1
21.2
R-L
19.2
30.4
15.4
19.3
29.5
18.5
21.1
22.7
45.5
40.9
19.8
20.2
20.1
Concentration
(Recovery
Corrected)
[ng/g]
22.1
13.8
21.5
15.3
16.0
43.8
41.6
27.2
39.8
33.0
57.2
36.5
39.1
22.1
21.9
25.6
6.08
5.82
6.70
3.30
24.9
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
152
101
101
185
83.7
266
395
149
179
146
269
288
136
200
165
80.2
46.7
40.2
48.7
17.1
142
A-82
-------
Table A-33. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13Ce-TeCB (Recovery 7.6% Lipid
Lipid Recovery1* Corrected) Content)
Laboratory" % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
29.6
44.0
0.00d
0.00d
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
0.00
0.00
0.00
0.00
0.473
0.856
0.902
0.717
4.58
0.00
0.00
3.42
4.57
2.21
3.44
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery <15%, R-H: rejected, recovery >120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-83
-------
Table A-34. 1,2,4-Trichlorobenzene Tissue Concentrations for Fundulus heteroclitus.
Station
A
Comp #1
Comp #2
Comp #3
Comp #4
Comp #4
1
Comp #1
B
Comp #1
Comp #1
Comp #2
Comp #3
Comp #3
Comp #4
Comp #5
Comp #6
C
Comp #1
Comp #1
D
Comp #1
Comp #2
Comp #2
Comp #2
Comp #3
E
Comp #1
Laboratory"
Bat E
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/1 5
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
ERL-D 5/17
Bat E
Bat E
Bat E
ERL-D 5/15
Bat E
ERL-D 5/17
ERL-D 5/1 7
Bat E
ERL-D 5/15
ERL-D 5/15
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
Lipid
%
0.975
1.01
1.42
0.612
1.42
1.24
0.794
1.37
0.615
1.58
1.54
0.954
2.04
0.825
0.994
2.18
0.930
1.03
0.988
1.30
1.31
13C8-TeCB
Recovery"
%
30.7
18. 1C
30.8
16.6
18.4
17.7
24.1
21.2
R-L
19.2
30.4
15.4
19.3
29.5
18.5
21.1
22.7
45.5
40.9
19.8
20.2
20.1
Concentration
(Recovery
Corrected)
[ng/g]
110
102
126
137
138
237
167
169
230
174
333
203
266
141
114
126
38.0
55.4
66.1
38.0
167
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
846
642
667
1494
649
1350
1439
845
1099
830
1636
1484
986
1145
744
434
174
286
380
125
872
A-84
-------
Table A-34. Continued.
Station
Blank
Blank -
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13Ce-TeCB (Recovery 7.6% Lipid
Lipid Recovery6 Corrected) Content)
Laboratory* % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ELD-D 5/17
ELD-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
29.6
44.0
0.00d
0.00d
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
12.7
10.5
14.7
35.6
12.8
12.6
17.6
17.0
1.90
0.00
0.00
1.02
1.05
3.11
3.12
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery <15%, R-H: rejected, recovery >120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-85
-------
Table A-35. 1,2,4.5- and 1,2,3,5- Tetrachlorobenzene Tissue Concentrations for Fundulus
heteroclitus.
Station
A
Comp #1
Comp #2
Comp #3
Comp #4
Comp #4
1
Comp #1
B
Comp #1
Comp #1
Comp #2
Comp #3
Comp #3
Comp #4
Comp #5
Comp #6
C
Comp #1
Comp #1
D
Comp #1
Comp # 2
Comp #2
Comp #2
Comp #2
E
Comp #1
Laboratory"
Bat E
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/1 5
ERL-D 5/1 5
ERL-D 5/17
ERL-D 5/17
ERL-D 5/1 7
Bat E
Bat E
Bat E
ERL-D 5/1 5
Bat E
ERL-D 5/17
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/1 5
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
Lipid
%
0.975
1.01
1.42
0.612
1,42
1.24
0.794
1,37
0.615
1.58
1.54
0.954
2.04
0.825
0.994
2.18
0.930
1.03
0.988
1.30
1.31
13C8-TeCB
Recovery*
%
30.7
18. 1C
30.8
16.6
18.4
17.7
24.1
21.2
R-L
19.2
30.4
15.4
19.3
29.5
18.5
21.1
22.7
45.5
40.9
19.8
20,2
20.1
Concentration
(Recovery
Corrected)
[ng/g]
100
189
104
276
275
453
356
387
190
177
263
404
164
331
303
131
86.9
99.9
103
54,9
310
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
769
1410
549
3408
1464
2767
3393
2138
907
845
1291
3206
606
3035
2305
452
697
726
780
312
1789
A-86
-------
Table A-35. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank -
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13Ca-TeCB (Recovery 7.6% Lipid
Lipid Recovery11 Corrected) Content)
Laboratory" % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
29.6
44.0
0.00d
0.00d
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
1.38
0.799
1.14
1.81
1.92
1.60
1.85
1.92
1.86
3.36
1.03
0.958
0.00
1.39
0.778
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery <15%, R-H: rejected, recovery >120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-87
-------
Table A-36. 1,2,3.4- Tetrachlorobenzene Tissue Concentrations for Fundulus heteroclitus.
Station
A
Comp #1
Comp #2
Comp #3
Comp #4
Comp #4
1
Comp #1
B
Comp #1
Comp #1
Comp #2
Comp #3
Comp #3
Comp #4
Comp #5
Comp #6
C
Comp #1
Comp #1
D
Comp #1
Comp f 2
Comp #2
Comp #2
Comp #3
E
Comp #1
Laboratory"
Bat E
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/15
ERL-D 5/15
ERL-D 5/1 7
ERL-D 5/17
ERL-D 5/17
BatE
Bat E
Bat E
ERL-D 5/15
BatE
ERL-D 5/17
ERL-D 5/17
BatE
ERL-D 5/15
ERL-D 5/15
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
Lipid
%
0.975
1.01
1.42
0.612
1.42
1.24
0.794
1.37
0.615
1.58
1.54
0.954
2.04
0.825
0.994
2.18
0.930
1.03
0.988
1.30
1.31
13Ce-TeCB
Recovery11
%
30.7
18.1C
30.8
16.6
18.4
17.7
24.1
21.2
R-L
19.2
30.4
15.4
19.3
29.5
18.5
21.1
22.7
45.5
40.9
19.8
20.2
20.1
Concentration
(Recovery
Corrected)
[ng/g]
116
72.7
124
90.8
78.5
189
133
151
243
227
343
191
215
131
119
172
35.8
47.6
56.5
27.9
127
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
897
543
659
1121
417
1155
1268
835
1165
1088
1688
1517
798
1202
906
597
288
347
431
160
734
A-88
-------
Table A-36. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank *
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13C6-TeCB (Recovery 7.6% Lipid
Lipid Recovery6 Corrected) Content)
Laboratory" % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
29.6
44.0
0.00d
0.00d
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
0.00
0.00
0.361
0.981
0.888
0.647
0.640
0.686
0.954
0.00
1.57
1.73
0.00
1.49
0.467
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery <15%, R-H: rejected, recovery >120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-89
-------
Table A-37. Pentachlorobenzene Tissue Concentrations for Fundulus heteroclitus.
Station "
A
Comp #1
Comp #2
Comp #3
Comp #4
Comp #4
1
Comp #1
B
Comp #1
Comp #1
Comp #2
Comp #3
Comp #3
Comp #4
Comp #5
Comp #6
C
Comp #1
Comp #1
D
Comp #1
Comp #2
Comp #2
Comp #2
Comp #3
E
Comp #1
Laboratory'
Bat E
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
ERL-D 5/17
Bat E
Bat E
Bat E
ERL-D 5/15
Bat E
ERL-D 5/17
ERL-D 5/17
Bat E
ERL-D 5/15
ERL-D 5/15
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
Lipid
%
0.975
1.01
1.42
0.612
1.42
1.24
0.794
1.37
0.615
1.58
1.54
0.954
2.04
0.825
0.994
2.18
0.930
1.03
0.988
1.30
1.31
13Ce-HCB
Recovery1"
%
26.7
19. 9e
28.3
17.7
17.6
18.2
27.2
22.7
16.3
18.8
29.7
15.6
20.3
30.2
19.5
25.1
25.2
49.9
46.4
21.7
23.2
21.9
Concentration
(Recovery
Corrected)
[ng/g]
705
483
769
620
685
1340
806
1060
1400
1390
1320
1280
899
871
962
929
715
251
257
265
128
688
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
5479
3631
4105
7693
3664
8210
7710
5878
17295
6676
6340
6307
7158
3237
8857
7099
2486
2047
1893
2035
745
3989
A-90
-------
Table A-37. Continued.
Station"
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13Cfl-HCB (Recovery 7.6% Lipid
Lipid Recovery1* Corrected) Content)
Laboratory" % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1 /24
ERL-D 1 /30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
31.5
46.2
0.00d
0.00d
55.6
22.8
63.8
62.9
50.8
57.2
36.9
32.2
29.5
24.6
37.6
35.1
34.0
0.456
0.425
0.507
0.480
0.481
0.241
0.838
0.588
2.47
3.74
0.967
1.38
1.35
3.63
0.779
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery <15%, R-H: rejected, recovery >120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-91
-------
Table A-38. Hexachlorobenzene Tissue Concentrations for Fundulus heteroclitus.
Station
A
Comp #1
Comp #2
Comp #3
Comp #4
Comp #4
1
Comp #1
B
Comp #1
Comp #1
Comp #2
Comp #3
Comp #3
Comp #4
Comp #5
Comp #6
C
Comp #1
Comp #1
D
Comp #1
Comp #2
Comp #2
Comp #2
Comp #3
E
Comp #1
Laboratory*
Bat E
ERL-D 5/17
BatE
ERL-D 5/15
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
ERL-D 5/17
BatE
BatE
BatE
ERL-D 5/15
Bat E
ERL-D 5/1 7
ERL-D 5/17
BatE
ERL-D 5/15
ERL-D 5/15
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
Lipid
%
0.975
1.01
1.42
0.612
1.42
1.24
0.794
1.37
0.615
1.58
1.54
0.954
2.04
0.825
0.994
2.18
0.930
1.03
0.988
1.30
1.31
13C8-HCB
Recovery6
%
26.7
19. 9C
28.3
17.7
17.6
18.2
27.2
22.7
16.3
18.8
29.7
15.6
20.3
30.2
19.5
25.1
25.2
49.9
46.4
21.7
23.2
21.9
Concentration
(Recovery
Corrected)
[ng/g]
644
528
564
844
1640
1050
732
753
805
719
633
648
528
475
703
714
361
201
200
188
152
237
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
4996
3970
3002
10475
8775
6433
7002
4175
9942
3443
3030
3183
4203
1758
6472
5456
1248
1639
1472
1443
886
1372
A-92
-------
Table A-38. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank '
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13Ce-HCB (Recovery 7.6% Lipid
Lipid Recovery11 Corrected) Content)
Laboratory" % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
31.5
46.2
0.00d
0.00d
55.6
22.8
63.8
62.9
50.8
57.2
36.9
32.2
29.5
24.6
37.6
35.1
34.0
0.458
0.347
0.446
0.552
0.393
0.318
0.594
0.570
3.78
1.33
6.06
3.27
2.74
2.43
2.25
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery <15%, R-H: rejected, recovery >120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-93
-------
Table A-39. Hexachloroethane Tissue Concentrations for Callinectes sapidus.
Station
A
Comp #1
Comp #2
Comp #3
1
Comp #1
Comp #1
Comp #2
Comp #3
Comp #4
B
Comp #1
Comp #1
Comp #1
Comp #2
Comp #2
C
Comp #1
Comp #1
Comp #1
Comp #2
D
Comp #1
Comp #1
Comp #2
E
Comp #1
Comp #1
Comp #1
Comp #2
Comp #3
Laboratory"
ERL-D 1/30
Bat FG
ERL-D 1/30
Bat FG
ERL-D 5/15
Bat FG
ERL-D 1/30
Bat E
Bat FG
Bat FG
ERL-D 5/15
ERL-D 1/30
ERL-D 4/23
Bat FG
ERL-D 1/3
ERL-D 1/24
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/3
Bat E
ERL-D 1/30
Lipid
%
0.575
2.49
0.916
0.902
0.416
1.22
1.49
1.09
2.59
1.93
1.02
0.953
1.95
1.56
1.83
0.960
2.22
1.25
1.57
1.10
0.953
1.06
3.36
1.85
Concentration
Concentration (Blank Corrected,
"C^HCE (Recovery 7.6% Lipid
Recovery*1 Corrected) Content)
% [ng/g] [ng/g]
28.9
37.2
24.5
42.2
8.61C
49.0
29.9
46.1
27.1
62.3
22.2
29.7
R-L
44.4
48.2
18.7
23.6
16.2
33.2
26.8
48.8
36.8
47.5
41.2
27.8
4.83
14.2
5.03
13.7
0.00
12.5
5.90
11.9
15.6
10.8
4.36
7.18
21.1
9.25
7.82
4.24
14.9
4.43
4.85
16.6
5.14
5.52
15.8
5.47
23.7
10.2
16.6
24.0
0.00
10.3
14.6
7.31
13.9
0.00
5.22
30.9
39.9
30.3
19.9
9.55
13.9
8.49
8.79
39.7
16.8
17.8
11.2
10.0
A-94
-------
Table A-39. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank -
Blank
Blank
Concentration
Concentration (Blank Corrected,
13d-HCE (Recovery 7.6% Lipid
Lipid Recovery1" Corrected) Content)
Laboratory1 % % [ng/g] [ng/g]
ERL-D 1 /3
ERL-D 1/3
ERL-D 1 /24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/1 7
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
29.3
42.4
0.00d
0.00d
58.8
. 23.3
62.7
64.1
51.1
59.1
41.2
R-L
37.5
31.4
45.5
46.9
52.8
4.01
3.63
4.12
0.00
3.21
2.91
3.38
3.01
11.9
8.41
9.50
12.7
11.1
11.5
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery < 15%, R-H: rejected, recovery > 120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-95
-------
Table A-40. Tetrachlorobutadiene #1 Tissue Concentrations for Callinectes sapidus.
Station
A
Comp #1
Comp #2
Comp #3
1
Comp #1
Comp #1
Comp #2
Comp #3
Comp #4
B
Comp #1
Comp #1
Comp #1
Comp #2
Comp #2
C
Comp #1
Comp #1
Comp #1
Comp #2
D
Comp #1
Comp #3
Comp #2
E
Comp #1
Comp #1
Comp #1
Comp #2
Comp #3
Laboratory*
ERL-D 1/30
Bat FG
ERL-D 1/30
Bat FG
ERL-D 5/15
Bat FG
ERL-D 1/30
Bat E
Bat FG
Bat FG
ERL-D 5/15
ERL-D 1/30
ERL-D 4/23
Bat FG
ERL-D 1/3
ERL-D 1/24
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/3
Bat E
ERL-D 1/30
Lipid
%
0.575
2.49
0.916
0.902
0.416
1.22
1.49
1.09
2.59
1.93
1.02
0.953
1.95
1.56
1.83
0.960
2.22
1.25
1.57
1.10
0.953
1.06
3.36
1.85
Concentration
Concentration (Blank Corrected,
13Ce-TeCB (Recovery 7.6% Lipid
Recovery11 Corrected) Content)
% [ng/g] [ng/g]
33.8
27.8
30.4
27.4
9.07C
32.2
33.8
28.8
18.7
42.4
24.7
31.7
16.4
24.0
55.3
22.0
28.3
17.2
37.7
32.1
27.4
43.0
50.1
27.6
32.9
0.00
4.57
0.559
8.79
0.00
3.48
1.45
0.00
17.7
17.4
3.75
4.09
3.12
29.0
7.86
6.61
0.00
2.87
1.21
1.47
13.8
1.85
1.72
9.57
2.62
0.00
13.9
4.64
74.1
0.00
21.7
7.40
0.00
51.9
51.1
14.8
30.5
24.9
113
38.3
27.5
0.00
9.83
7.36
7.12
95.3
14.8
12.3
21.6
10.8
A-96
-------
Table A-40. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13C6-TeCB (Recovery 7.6% Lipid
Lipid Recovery6 Corrected) Content)
Laboratory" % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
29.6
44.0
0.00d
0.00d
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery <15%, R-H: rejected, recovery >120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-97
-------
Table A-41. Tetrachlorobutadiene #2 Tissue Concentrations for Callinectes sapidus.
Station
A
Comp #1
Comp #2
Comp #3
1
Comp #1
Comp #1
Comp #2
Comp #3
Comp #4
B
Comp #1
Comp #3
Comp #1
Comp #2
Comp #2
C
Comp #1
Comp #1
Comp #1
Comp #2
D
Comp #1
Comp #1
Comp #2
E
Comp #1
Comp #1
Comp #1
Comp #2
Comp #3
Laboratory*
ERL-D 1/30
Bat FG
ERL-D 1/30
Bat FG
ERL-D 5/15
Bat FG
ERL-D 1/30
Bat E
Bat FG
Bat FG
ERL-D 5/15
ERL-D 1/30
ERL-D 4/23
Bat FG
ERL-D 1/3
ERL-D 1/24
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/3
Bat E
ERL-D 1/30
Lipid
%
0.575
2.49
0.916
0.902
0.416
1.22
1.49
1.09
2.59
1.93
1.02
0.953
1.95
1.56
1.83
0.960
2.22
1.25
1.57
1.10
0.953
1.06
3.36
1.85
Concentration
Concentration (Blank Corrected,
13Ce-TeCB (Recovery 7.6% Lipid
Recovery11 Corrected) Content)
% Ing/g] [ng/g]
33.8
27.8
30.4
27.4
9.07°
32.2
33.8
28.8
18.7
42.4
24.7
31.7
16.4
24.0
55.3
22.0
28.3
17.2
37.7
32.1
27.4
43.0
50.1
27.6
32.9
1.21
7.81
3.12
13.9
5.19
6.70
13.2
6.03
38.4
38.6
27.2
33.2
26.2
47.9
46.4
42.3
1.19
7.39
9.45
13.0
18.7
13.4
13.1
17.7
22.2
16.0
23.8
25.9
117
94.8
41.7
67.3
42.0
113
113
107
247
209
187
226
176
9.42
25.3
57.5
62.9
129
107
93.9
40.0
91.2
A-98
-------
Table A-41. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13Ce-TeCB (Recovery 7.6% Lipid
Lipid Recovery4* Corrected) Content)
Laboratory" % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
29.6
44.0
0.00d
0.00d
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery < 15%, R-H: rejected, recovery > 120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-99
-------
Table A-42. Pentachlorobutadiene #1 Tissue Concentrations for Callinectes sapidus.
Station
A
Comp #1
Comp #2
Comp #3
1
Comp #1
Comp #1
Comp #2
Comp #3
Comp #4
B
Comp #1
Comp #1
Comp #1
Comp #2
Comp #2
C
Comp #1
Comp #1
Comp #1
Comp #2
D
Comp #1
Comp #J
Comp #2
E
Comp #1
Comp #1
Comp #1
Comp #2
Comp #3
Laboratory*
ERL-D 1/30
Bat FG
ERL-D 1/30
Bat FG
ERL-D 5/15
Bat FG
ERL-D 1/30
Bat E
Bat FG
Bat FG
ERL-D 5/15
ERL-D 1/30
ERL-D 4/23
Bat FG
ERL-D 1/3
ERL-D 1/24
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/3
Bat E
ERL-D 1/30
Lipid
%
0.575
2.49
0.916
0.902
0.416
1.22
1.49
1.09
2.59
1.93
1.02
0.953
1.95
1.56
1.83
0.960
2.22
1.25
1.57
1.10
0.953
1.06
3.36
1.85
13C8-TeCB
Recovery1"
%
33.8
27.8
30.4
27.4
9.07C
32.2
33.8
28.8
18.7
42.4
24.7
31.7
16.4
24.0
55.3
22.0
28.3
17.2
37.7
32.1
27.4
43.0
50.1
27.6
32.9
Concentration
(Recovery
Corrected)
[ng/g]
6.31
62.4
5.59
43.7
9.30
45.3
21.0
32.0
272
282
55.4
43.3
35.8
347
72.0
64.1
0.00
41.4
9.64
12.3
172
22.0
22.0
152
19.1
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
83.4
190
46.4
366
170
280
107
221
797
827
218
323
285
1351
351
266
0.00
141
58.6
59.5
1186
175
158
343
78.5
A-100
-------
Table A-42. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank -
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13C6-TeCB (Recovery 7.6% Lipid
Lipid Recovery6 Corrected) Content)
Laboratory* % % lng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1 /30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
29.6
44.0
0.00"
0.00d
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
2.19
0.00
0.00
0.00
0.00
0.00
0.00
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery <15%, R-H: rejected, recovery >120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-101
-------
Table A-43. Pentachlorobutadiene #2 Tissue Concentrations for Callinectes sapidus.
Station
A
Comp #1
Comp #2
Comp #3
1
Comp #1
Comp #1
Comp #2
Comp #3
Comp #4
B
Comp #1
Comp #1
Comp #1
Comp #2
Comp #2
C
Comp #1
Comp #1
Comp #1
Comp #2
D
Comp #1
Comp #1
Comp #2
E
Comp #1
Comp #1
Comp #1
Comp #2
Comp #3
Laboratory*
ERL-D 1/30
Bat FG
ERL-D 1/30
Bat FG
ERL-D 5/15
Bat FG
ERL-D 1/30
Bat E
Bat FG
Bat FG
ERL-D 5/15
ERL-D 1/30
ERL-D 4/23
Bat FG
ERL-D 1/3
ERL-D 1/24
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/3
Bat E
ERL-D 1/30
Lipid
%
0.575
2.49
0.916
0.902
0.416
1.22
1.49
1.09
2.59
1.93
1.02
0.953
1.95
1.56
1.83
0.960
2.22
1.25
1.57
1.10
0.953
1.06
3.36
1.85
Concentration
Concentration (Blank Corrected,
13Ce-TeCB (Recovery 7.6% Lipid
Recovery11 Corrected) Content)
% [ng/g] [ng/g]
33.8
27.8
30.4
27.4
9.07C
32.2
33.8
28.8
18.7
42.4
24.7
31.7
16.4
24.0
55.3
22.0
28.3
17.2
37.7
32.1
27.4
43.0
50.1
27.6
32.9
0.00
1.91
0.00
2.20
0.00
1.01
0.687
1.76
7.97
8.26
1.10
0.969
0.00
11.9
2.07
2.04
0.00
2.34
0.00
0.478
3.78
0.454
0.00
2.34
0.00
0.00
5.42
0.00
17.40
0.00
5.45
3.50
11.33
22.99
23.84
4.33
7.22
0.00
45.85
10.08
8.47
0.00
7.55
0.00
2.31
25.18
3.62
0.00
4.99
0.00
A-102
-------
Table A-43. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13Ce-TeCB (Recovery 7.6% Lipid
Lipid Recovery11 Corrected) Content)
Laboratory" % % [ng/g] [ng/g]
ERL-D 1 13
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
29.6
44.0
0.00d
0.00d
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.948
0.00
0.00
0.00
0.00
0.00
0.00
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery < 15%, R-H: rejected, recovery > 120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-103
-------
Table A-44. Hexachlorobutadiene Tissue Concentrations for Callinectes sapidus.
Station
A
Comp #1
Comp #2
Comp #3
1
Comp #1
Comp #1
Comp #2
Comp #3
Comp #4
B
Comp #1
Comp #1
Comp #1
Comp #2
Comp #2
C
Comp #1
Comp #1
Comp #1
Comp #2
D
Comp #1
Comp #1
Comp #2
E
Comp #1
Comp #1
Comp #1
Comp #2
Comp #3
Laboratory*
ERL-D 1/30
Bat FG
ERL-D 1/30
Bat FG
ERL-D 5/15
Bat FG
ERL-D 1/30
Bat E
Bat FG
Bat FG
ERL-D 5/15
ERL-D 1/30
ERL-D 4/23
Bat FG
ERL-D 1/3
ERL-D 1/24
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/3
Bat E
ERL-D 1/30
Lipid
%
0.575
2.49
0.916
0.902
0.416
1.22
1.49
1.09
2.59
1.93
1.02
0.953
1.95
1.56
1.83
0.960
2.22
1.25
1.57
1.10
0.953
1.06
3.36
1.85
13C6-TeCB
Recovery^
%
33.8
27.8
30.4
27.4
9.07C
32.2
33.8
28.8
18.7
42.4
24.7
31.7
16.4
24.0
55.3
22.0
28.3
17.2
37.7
32.1
27.4
43.0
50.1
27.6
32.9
Concentration
(Recovery
Corrected)
[ng/g]
41.6
79.3
24.2
52.2
56.7
0.947
85.4
47.4
185
212
132
136
116
285
225
185
3.64
39.9
32.2
87.1
141
59.9
62.3
136
56.6
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
451
231
139
410
899
0.00
397
306
533
612
490
958
865
1097
1060
737
0.00
125
150
385
950
418
393
300
202
A-104
-------
Table A-44. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13C6-TeCB (Recovery 7.6% Lipid
Lipid Recovery6 Corrected) Content)
Laboratory" % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
29.6
44.0
0.00d
0.00d
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
5.04
2.81
4.93
9.90
7.46
7.28
11.2
11.2
11.5
1.50
2.11
0.00
6.30
2.23
0.840
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery <15%, R-H: rejected, recovery >120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-105
-------
Table A-45. 1,2,3-Trichlorobenzene Tissue Concentrations for Callinectes sapidus.
Station
A
Comp #1
Comp #2
Comp #3
1
Comp #1
Comp #1
Comp #2
Comp #3
Comp #4
B
Comp #1
Comp #1
Comp #1
Comp #2
Comp #2
C
Comp #1
Comp #1
Comp #1
Comp #2
D
Comp #1
Comp #1
Comp #2
E
Comp #1
Comp #1
Comp #1
Comp #2
Comp #3
Laboratory"
ERL-D 1/30
Bat FG
ERL-D 1/30
Bat FG
ERL-D 5/15
Bat FG
ERL-D 1/30
Bat E
Bat FG
Bat FG
ERL-D 5/15
ERL-D 1/30
ERL-D 4/23
Bat FG
ERL-D 1/3
ERL-D 1/24
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/3
Bat E
ERL-D 1/30
Lipid
%
0.575
2.49
0.916
0.902
0.416
1.22
1.49
1.09
2.59
1.93
1.02
0.953
1.95
1.56
1.83
0.960
2.22
1.25
1.57
1.10
0.953
1.06
3.36
1.85
Concentration
Concentration (Blank Corrected,
13C6-TeCB (Recovery 7.6% Lipid
Recoveryb Corrected) Content)
% [ng/g] [ng/g]
33.8
27.8
30.4
27.4
9.07°
32.2
33.8
28.8
18.7
42.4
24.7
31.7
16.4
24.0
55.3
22.0
28.3
17.2
37.7
32.1
27.4
43.0
50.1
27.6
32.9
3.24
44.9
9.71
24.5
18.8
30.0
32.4
12.6
53.2
58.3
41.8
52.8
45.8
87.2
69.0
61.7
5.23
18.2
14.7
23.5
50.8
22.5
24.0
29.1
31.5
38.0
129
77.5
184
337
171
163
69.7
148
163
163
391
362
330
334
255
38.5
53.4
87.1
112
333
176
169
59.9
128
A-106
-------
Table A-45. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13Ce-TeCB (Recovery 7.6% Lipid
Lipid Recovery6 Corrected) Content)
Laboratory' % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1 /30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
29.6
44.0
0.00d
0.00d
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
0.00
0.00
0.00
0.00
0.473
0.856
0.902
0.717
4.58
0.00
0.00
3.42
4.57
2.21
3.44
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery < 15%, R-H: rejected, recovery > 120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-107
-------
Table A-46. 1,2,4-Trichlorobenzene Tissue Concentrations for Callinectes sapidus.
Station
A
Comp #1
Comp #2
Comp #3
1
Comp #1
Comp #1
Comp #2
Comp #3
Comp #4
B
Comp #1
Comp #1
Comp #1
Comp #2
Comp #2
C
Comp #1
Comp #1
Comp #1
Comp #2
D
Comp #1
Comp #1
Comp #2
E
Comp #1
Comp #1
Comp #1
Comp #2
Comp #3
Laboratory*
ERL-D 1/30
Bat FG
ERL-D 1/30
Bat FG
ERL-D 5/15
Bat FG
ERL-D 1/30
Bat E
Bat FG
Bat FG
ERL-D 5/15
ERL-D 1/30
ERL-D 4/23
Bat FG
ERL-D 1/3
ERL-D 1/24
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/3
Bat E
ERL-D 1/30
Lipid
%
0.575
2.49
0.916
0.902
0.416
1.22
1.49
1.09
2.59
1.93
1.02
0.953
1.95
1.56
1.83
0.960
2.22
1.25
1.57
1.10
0.953
1.06
3.36
1.85
13Ce-TeCB
Recovery*1
%
33.8
27.8
30.4
27.4
9.07C
32.2
33.8
28.8
18.7
42.4
24.7
31.7
16.4
24.0
55.3
22.0
28.3
17.2
37.7
32.1
27.4
43.0
50.1
27.6
32.9
Concentration
(Recovery
Corrected)
[ng/g]
26.7
178
47.1
89.6
93.6
136
134
50.2
337
354
261
294
238
534
432
352
29.7
97.1
80.2
115
260
111
113
171
177
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
132
539
252
743
1405
838
598
340
985
1034
962
2066
1765
2076
2023
1393
103
327
386
476
1786
752
691
383
659
A-108
-------
Table A-46. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13Ce-TeCB (Recovery 7.6% Lipid
Lipid Recovery6 Corrected) Content)
Laboratory" % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ELD-D 5/17
ELD-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
29.6
44.0
0.00d
0.00d
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
12.7
10.5
14.7
35.6
12.8
12.6
17.6
17.0
1.90
0.00
0.00
1.02
1.05
3.11
3.12
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery < 15%, R-H: rejected, recovery > 120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-109
-------
Table A-47. 1,2,4,5- and 1,2,3,5-Tetrachlorobenzene Tissue Concentrations for
Callinectes sapidus.
Station
A
Comp #1
Comp #2
Comp #3
1
Comp #1
Comp #1
Comp #2
Comp #3
Comp #4
B
Comp #]
Comp #1
Comp #1
Comp #2
Comp #2
C
Comp #1
Comp #1
Comp #1
Comp #2
D
Comp #1
Comp #1
Comp #2
E
Comp #1
Comp #1
Comp #1
Comp #2
Comp #3
Laboratory*
ERL-D 1/30
Bat FG
ERL-D 1/30
Bat FG
ERL-D 5/15
Bat FG
ERL-D 1/30
Bat E
Bat FG
Bat FG
ERL-D 5/1 5
ERL-D 1/30
ERL-D 4/23
Bat FG
ERL-D 1/3
ERL-D 1/24
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/3
Bat E
ERL-D 1/30
Lipid
%
0.575
2.49
0.916
0.902
0.416
1.22
1.49
1.09
2.59
1.93
1.02
0.953
1.95
1.56
1.83
0.960
2.22
1.25
1.57
1.10
0.953
1.06
3.36
1.85
13Ce-TeCB
Recovery6
%
33.8
27.8
30.4
27.4
9.07C
32.2
33.8
28.8
18.7
42.4
24.7
31.7
16.4
24.0
55.3
22.0
28.3
17.2
37.7
32.1
27.4
43.0
50.1
27.6
32.9
Concentration
(Recovery
Corrected)
[ng/g]
38.8
127
68.0
59.1
124
98.1
236
34.9
126
123
273
310
291
197
510
456
50.7
39.1
90.9
168
110
155
156
67.5
213
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
492
384
551
487
2237
603
1196
234
366
357
1069
2298
2308
763
2477
1887
389
129
543
806
751
1224
1107
150
869
A-110
-------
Table A-47. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13C8-TeCB (Recovery 7.6% Lipid
Lipid Recovery1" Corrected) Content)
Laboratory" % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/1 5
ERL-D 5/1 5
ERL-D 5/17
ERL-D 5/1 7
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
29.6
44.0
0.00d
0.00d
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
1.38
0.799
1.14
1.81
1.92
1.60
1.85
1.92
1.86
3.36
1.03
0.958
0.00
1.39
0.778
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery < 15%, R-H: rejected, recovery > 120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-111
-------
Table A-48. 1,2,3,4-Tetrachlorobenzene Tissue Concentrations for Callinectes sapidus.
Station
A
Comp #1
Comp #2
Comp #3
1
Comp #1
Comp #1
Comp #2
Comp #3
Comp #4
B
Comp #1
Comp #1
Comp #1
Comp #2
Comp #2
C
Comp #1
Comp #1
Comp #1
Comp #2
D
Comp #1
Comp #1
Comp #2
E
Comp #1
Comp #1
Comp #1
Comp #2
Comp #3
Laboratory"
ERL-D 1/30
Bat FG
ERL-D 1/30
Bat FG
ERL-D 5/15
Bat FG
ERL-D 1/30
Bat E
Bat FG
Bat FG
ERL-D 5/15
ERL-D 1/30
ERL-D 4/23
Bat FG
ERL-D 1/3
ERL-D 1/24
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/3
Bat E
ERL-D 1/30
Lipid
%
0.575
2.49
0.916
0.902
0.416
1.22
1.49
1.09
2.59
1.93
1.02
0.953
1.95
1.56
1.83
0.960
2.22
1.25
1.57
1.10
0.953
1.06
3.36
1.85
13C8-TeCB
Recovery11
%
33.8
27.8
30.4
27.4
9.07C
32.2
33.8
28.8
18.7
42.4
24.7
31.7
16.4
24.0
55.3
22.0
28.3
17.2
37.7
32.1
27.4
43.0
50.1
27.6
32.9
Concentration
(Recovery
Corrected)
[ng/g]
21.7
167
38.8
86.5
73.9
137
115
52.9
176
169
115
189
168
264
284
255
29.5
48.7
50.8
104
145
90.1
89.3
88.5
125
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
280
507
318
721
1340
848
584
363
514
493
451
1404
1336
1025
1381
1057
229
164
306
501
996
714
636
198
511
A-112
-------
Table A-48. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank .
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13C6-TeCB (Recovery 7.6% Lipid
Lipid Recovery11 Corrected) Content)
Laboratory" % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/1 5
ERL-D 5/15
ERL-D 5/1 7
ERL-D 5/1 7
Bat A
Bat B
BatC
Bat D
Bat E
Bat F
Bat G
29.6
44.0
0.00d
0.00d
52.9
21.5
61.9
59.1
48.5
54.9
31.4
23.6
27.8
23.5
27.8
29.1
28.9
0.00
0.00
0.361
0.981
0.888
0.647
0.640
0.686
a.
0.954
0.00
1.57
1.73
0.00
1.49
0.467
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery <15%, R-H: rejected, recovery >120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-113
-------
Table A-49. Pentachlorobenzene Tissue Concentrations for Callinectes saoidus.
Station
A
Comp #1
Comp #2
Comp #3
1
Comp #1
Comp #1
Comp #2
Comp #3
Comp #4
B
Comp #1
Comp #1
Comp #1
Comp #2
Comp #2
C
Comp #1
Comp #1
Comp #1
Comp #2
D
Comp #1
Comp #1
Comp #2
E
Comp #1
Comp #1
Comp #1
Comp #2
Comp #3
Laboratory*
ERL-D 1/30
Bat FG
ERL-D 1/30
Bat FG
ERL-D 5/15
Bat FG
ERL-D 1/30
Bat E
Bat FG
Bat FG
ERL-D 5/1 5
ERL-D 1/30
ERL-D 4/23
Bat FG
ERL-D 1/3
ERL-D 1/24
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/3
Bat E
ERL-D 1/30
Lipid
%
0.575
2.49
0.916
0.902
0.416
1.22
1.49
1.09
2.59
1.93
1.02
0.953
1.95
1.56
1.83
0.960
2.22
1.25
1.57
1.10
0.953
1.06
3.36
1.85
13C8-HCB
Recovery6
%
38.1
28.1
33.4
30.8
9.83°
33.7
36.8
33.2
21.9
48.2
27.4
32.0
17.0
25,9
60.1
24.5
31.4
15.3
41.8
34.5
27.9
50.5
53.1
29.9
36.3
Concentration
(Recovery
Corrected)
[ng/gl
102
587
154
241
212
507
500
183
406
408
377
503
386
586
617
612
83.0
115
94.7
309
380
219
233
194
311
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
1342
1785
1274
2013
3864
3146
2548
1262
1185
1191
1483
3744
3074
2276
3003
2540
653
387
573
1493
2611
1742
1667
434
1276
A-114
-------
Table A-49. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13C8-HCB (Recovery 7.6% Lipid
Lipid Recovery6 Corrected) Content)
Laboratory" % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/1 7
Bat A
Bat B
BatC
Bat D
Bat E
Bat F
BatG
31.5
46.2
0.00d
0.00d
55.6
22.8
63.8
62.9
50.8
57.2
36.9
32.2
29.5
24.6
37.6
35.1
34.0
0.456
0.425
0.507
0.480
0.481
0.241
0.838
0.588
2.47
3.74
0.967
1.38
1.35
3.63
0.779
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery < 15%, R-H: rejected, recovery > 120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-115
-------
Table A-50. Hexachlorobenzene Tissue Concentrations for Callinectes sapidus.
Station
A
Comp #1
Comp #2
Comp #3
1
Comp #1
Comp #1
Comp #2
Comp #3
Comp #4
1164
B
Comp #1
Comp #1
Comp #1
Comp #2
Comp #2
C
Comp #1
Comp #1
Comp #1
Comp #2
D
Comp #1
Comp #1
Comp #2
E
Comp #1
Comp #1
Comp #1
Comp #2
Comp #3
Laboratory"
ERL-D 1/30
BatFG
ERL-D 1/30
BatFG
ERL-D 5/15
BatFG
ERL-D 1/30
Bat E
Bat FG
Bat FG
ERL-D 5/15
ERL-D 1/30
ERL-D 4/23
BatFG
ERL-D 1/3
ERL-D 1/24
ERL-D 1/30
BatFG
ERL-D 1/3
ERL-D 1/30
Bat FG
ERL-D 1/3
ERL-D 1/3
Bat E
ERL-D 1/30
Lipid
%
0.575
2.49
0.916
0.902
0.416
1.22
1.49
1.09
2.59
1.93
1.02
0.953
1.95
1.56
1.83
0.960
2.22
1.25
1.57
1.10
0.953
1.06
3.36
1.85
13Ce-HCB
Recovery6
%
38.1
28.1
33.4
30.8
9.83C
33.7
36.8
33.2
21.9
48.2
27.4
32.0
17.0
25.9
60.1
24.5
31.4
15.3
41.8
34.5
27.9
50.5
53.1
29.9
36.3
Concentration
(Recovery
Corrected)
[ng/g]
145
544
212
196
178
400
452
170
256
253
250
320
242
274
328
292
57.0
60.7
59.1
266
287
203
199
109
305
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
1910
1651
1755
1625
3244
2472
2303
1164
742
733
983
2381
1926
1056
1596
1211
448
197
357
1285
1961
1615
1423
239
1251
A-116
-------
Table A-50. Continued.
Station
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Concentration
Concentration (Blank Corrected,
13Ce-HCB (Recovery 7.6% Lipid
Lipid Recovery6 Corrected) Content)
Laboratory" % % [ng/g] [ng/g]
ERL-D 1/3
ERL-D 1/3
ERL-D 1/24
ERL-D 1/24
ERL-D 1/30
ERL-D 4/23
ERL-D 5/15
ERL-D 5/15
ERL-D 5/17
ERL-D 5/17
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
31.5
46.2
0.00d
0.00d
55.6
22.8
63.8
62.9
50.8
57.2
36.9
32.2
29.5
24.6
37.6
35.1
34.0
0.458
0.347
0.446
0.552
0,393
0.318
0.594
0.570
3.78
1.33
6.06
3.27
2.74
2.43
2.25
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R-L: rejected, recovery < 15%, R-H: rejected, recovery > 120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
Recovery was low because not all of the extract was subjected to GPC cleanup.
No surrogate added to these procedural blanks.
A-117
-------
Table A--51. Hexachloroethane Tissue Concentrations for Brevoortia patron us and
Micropoaan undulus.
Station
Laboratory*
Lipid
%
13C,-HCE
Recovery1"
%
Concentration
(Recovery
Corrected)
[ng/g]
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
Brevoortia patronus
C
Comp A
Comp B
Comp C
Comp D
Comp E
Comp F
Comp G
Comp G-
D
Comp A
Comp B
E
Comp A
Comp B
Comp B
Comp C
Comp D
Micropoaan
B
Comp A
C
Comp A-
Comp A
D
Comp A
Comp B
Comp B
Comp C
Bat A
Bat A
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat BCD
undulus
Bat A
Bat BCD
Bat BCD
Bat A
Bat A
Bat A
Bat BCD
2.77
2.56
3.46
6.15
3.28
3.22
3.14
2.34
3.22
2.06
3.05
2.37
1.54
1.53
1.24
1.51
3.01
0.964
45.5
32.7
21.9
48.2
51.8
48.9
31.2
65.9
33.2
27.7
31.0
30.8
25.4
30.7
26.0
31.2
57.6
R-L
R-L
52.1
37.9
35.5
36.0
105
45.2
231
164
116
129
102
180
109
168
123
127
130
61.2
28.0
18.8
108
134
36.6
69.0
280
75.4
272
355
248
286
221
549
232
580
279
289
382
248
85.2
48.7
245
311
203
A-118
-------
Table A-51. Continued.
-
Station
E
Comp A
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Laboratory"
Bat A
Bat A
Bat B
Bat C
Bat D
Bat E
BatF
Bat G
13C,-HCE
Lipid Recovery*
% %
1.26 51.4
41.2
R-L
37.5
31.4
45.5
46.9
52.8
Concentration
(Recovery
Corrected)
[ng/g]
64.9
11.9
8.41
9.50
12.7
11.1
11.5
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
326
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
R4_: rejected, recovery < 15%, R-H: rejected, recovery > 120%, R-l: rejected,
incorrect sample, R-P: rejected, procedural error during sample preparation.
A-119
-------
Table A-52. Tetrachlorobutadiene #1 Tissue Concentrations for Brevoortia patronus and
Micropogan undulus.
Station
Laboratory"
Lipid
%
'3C6-TeCB
Recovery
%
Concentration
(Recovery
Corrected)
[ng/g]
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
Brevoortia patronus
C
Comp A
Comp B
Comp C
Comp D
Comp E
Comp F"
Comp G
Comp G
D
Comp A
Comp B
E
Comp A
Comp B
Comp B
Comp C
Comp D
Micropoqan
B
Comp A
C
Comp A
Comp A
D
Comp A
Comp B
Comp B
Comp C
Bat A
Bat A
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat BCD
undulus
Bat A
Bat BCD
Bat BCD
Bat A
Bat A
Bat A
Bat BCD
2.77
2.56
3.46
6.15
3.28
3.22
3.14
2.34
3.22
2.06
3.05
2.37
1.54
1.53
1.24
1.51
3.01
0.964
37.1
28.0
25.3
48.2
39.0
39.1
21.2
46.5
27.8
30.0
22.0
25.4
23.6
21.8
24.4
37.1
41.7
17.4
28.6
40.6
23.6
25.9
8.90
23.7
7.20
37.0
27.4
22.1
21.4
19.7
36.1
17.9
31.1
30.4
23.4
37.4
12.9
3.87
2.08
0.00
16.4
25.1
38.8
10.3
24.4
70.4
15.8
45.7
63.5
52.2
51.8
47.7
117
42.2
115
75.8
58.3
120
63.7
19.2
12.7
0.00
82.5
63.4
98.0
81.2
A-120
-------
Table A-52. Continued.
Concentration
Concentration (Blank Corrected,
Station
E
Comp A
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Laboratory"
Bat A
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
13C6-TeCB
Lipid Recovery
% %
1.26 39.5
31.4
23.6
27.8
23.5
27.8
29.1
28.9
(Recovery
Corrected)
[ng/g]
16.9
0.00
0.00
0.00
0.00
0.00
0.00
0.00
7.6% Lipid
Content)
[ng/g]
102
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
A-121
-------
Table A-53. Tetrachlorobutadiene #2 Tissue Concentrations for Brevoortia patronus and
Micropoaan undulus.
Station Laboratory'
Lipid
13Ce-TeCB
Recovery
Concentration
(Recovery
Corrected)
[ng/gl
Concentration
(Blank Corrected,
7.6% Lipid
Content)
tng/g]
Brevoortia patronus
c
Comp A
Comp B
Comp C
Comp D
Comp E
Comp F
Comp G
Comp G
D
Comp A
Comp B
E
Comp A
Comp B
Comp B-
Comp C
Comp D
Bat A
Bat A
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat BCD
2.77
2.56
3.46
6.15
3.28
3.22
3.14
2.34
3.22
2.06
3.05
2.37
1.54
37.1
28.0
25.3
48.2
39.0
39.1
21.2
46.5
27.8
30.0
22.0
25.4
23.6
21.8
24.4
21.8
66.1
20.9
119
84.7
65.3
55.8
47.8
102
59.1
76.2
77.0
63.9
99.8
34.4
Micropoaan undulus
B
Comp A
C
Comp A
Comp A
D
Comp A
Comp B
Comp B
Comp C
Bat A
Bat BCD
Bat BCD
1.53
1.24
37.1
41.7
17.4
13.2
6.60
0.00
Bat A
Bat A
Bat A
Bat BCD
1.51
3.01
0.964
28.6
40.6
23.6
25.9
40.3
60.2
102
25.6
59.8
196
45.9
147
196
154
135
116
331
139
281
192
159
320
170
65.6
40.5
0.00
203
152
258
202
A-122
-------
Table A-53. Continued.
Station
E
Comp A
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Laboratory*
Bat A
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
13Ca-TeCB
Lipid Recovery
1.26 39.5
. 31.4
23.6
27.8
23.5
27.8
29.1
28.9
Concentration
Concentration (Blank Corrected,
(Recovery 7.6% Lipid
Corrected) Content)
[ng/g] [ng/g]
45.7 276
0.00
0.00
0.00
0,00
0.00
0.00
0.00
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
A-123
-------
Table A-54. Pentachlorobutadiene #1 Tissue Concentrations for Brevoortia patronus and
MicroooQan undulus.
Station
Laboratory'
Lipid
%
13C8-TeCB
Recovery
Concentration
(Recovery
Corrected)
[ng/g]
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
Brevoortia oatronus
c
Comp A
Comp B
Comp C
Comp D
Comp E
Comp F
Comp G
Comp G
D
Comp A
Comp B
E
Comp A
Comp B
Comp B
Comp C
Comp 0
Bat A
Bat A
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat BCD
2.77
2.56
3.46
6.15
3.28
3.22
3.14
2.34
3.22
2.06
3.05
2.37
1.54
37.1
28.0
25.3
48.2
39.0
39.1
21.2
46.5
27.8
30.0
22.0
25.4
23.6
21.8
24.4
334
1340
366
2020
1460
1340
1060
890
2280
1370
1870
1480
1370
2260
767
916
3977
803
2496
3382
3162
2565
2153
7404
3233
6898
3687
3413
7246
3784
Micropoaan undulus
B
Comp A
C
Comp A
Comp A
D
Comp A
Comp B
Comp B
Comp C
Bat A
Bat BCD
Bat BCD
1.53
1.24
37.1
41.7
17.4
173
120
11.6
Bat A
Bat A
Bat A
Bat BCD
1.51
3.01
0.964
28.6
40.6
23.6
25.9
826
1250
1600
555
858
734
69
4156
3155
4039
4373
A-124
-------
Table A-54. Continued.
Concentration
Concentration (Blank Corrected,
Station
E
Comp A
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Lipid
Laboratory1 %
Bat A 1.26
Bat A
Bat B
BatC
Bat D
Bat E
Bat F
Bat G
13Ca-TeCB
Recovery
%
39.5
. 31.4
23.6
27.8
23.5
27.8
29.1
28.9
(Recovery
Corrected)
[ng/g]
830
2.19
0.00
0.00
0.00
0.00
0.00
0.00
7.6% Lipid
Content)
[ng/g]
5004
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
A-125
-------
Table A-55. Pentachlorobutadiene #2 Tissue Concentrations for Brevoortia patronus and
Micropogan undulus.
___ Concentration
(Blank Corrected,
7.6% Lipid
Lipid Recovery Corrected) Content)
Station Laboratory* % % lng/g] [ng/g]
13C8-TeCB
Recovery
Concentration
(Recovery
Corrected)
Ing/g]
Brevoortia oatronus
c
Comp A
Comp B
Comp C
Comp D
Comp E
Comp F
Comp G
Comp G
D
Comp A
Comp B
E
Comp A
Comp B
Comp B
Comp C
Comp D
Bat A
Bat A
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat BCD
2.77
2.56
3.46
6.15
3.28
3.22
3.14
2.34
3.22
2.06
3.05
2.37
1.54
37.1
28.0
25.3
48.2
39.0
39.1
21.2
46.5
27.8
30.0
22.0
25.4
23.6
21.8
24.4
79.7
268
81.0
491
331
266
227
200
475
270
333
300
279
443
148
218
795
178
607
767
628
549
484
1542
637
1228
747
695
1420
730
Micropoaan undulus
B
Comp A
C
Comp A
Comp A
D
Comp A
Comp B
Comp B
Comp C
Bat A
Bat BCD
Bat BCD
1.53
1.24
37.1
41.7
17.4
18.7
9.39
2.48
Bat A
Bat A
Bat A
Bat BCD
1.51
3.01
0.964
28.6
40.6
23.6
25.9
111
111
151
49.6
92,2
56.7
14.4
558
280
381
390
A-126
-------
Table A-55. Continued.
Station
Laboratory1
Lipid
%
13Ca-TeCB
Recovery
Concentration
Concentration (Blank Corrected,
(Recovery 7.6% Lipid
Corrected) Content)
[ng/g] [ng/g]
E
Comp A
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Bat A
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
1.26 39.5
31.4
23.6
27.8
23.5
27.8
29.1
28.9
73.6 443
0.948
0.00
0.00
0.00
0.00
0.00
0.00
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
A-127
-------
Table A-56. Hexachlorobutadiene Tissue Concentrations for Brevoortia patronus and
Microppgan undulus.
Concentration
Concentration (Blank Corrected,
13Ce-TeCB (Recovery 7.6% Lipid
Lipid Recovery Corrected) Content)
Station Laboratory* % % [ng/g] [ng/g]
Brevoortia patronus
5368
13676
4298
5841
10349
11156
9625
8124
20190
11557
21717
10233
10307
21474
13061
c
Comp A
Comp B
Comp C
Comp D
Comp E
Comp F
Comp G
Comp G
D
Comp A
Comp B
E
Comp A
Comp B
Comp B
Comp C
Comp D
Bat A
Bat A
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat BCD
2.77
2.56
3.46
6.15
3.28
3.22
3.14
2.34
3.22
2.06
3.05
2.37
1.54
37.1
28.0
25.3
48.2
39.0
39.1
21.2
46.5
27.8
30.0
22.0
25.4
23.6
21.8
24.4
1960
4610
1960
4730
4470
4730
3980
3360
6220
4900
5890
4110
4140
6700
2650
Microooaan undulus
B
Comp A
C
Comp A
Comp A
D
Comp A
Comp B
Comp B
Comp C
Bat A
Bat BCD
Bat BCD
Bat A
Bat A
Bat A
Bat BCD
1.53
1.24
1.51
3.01
0.964
37.1
41.7
17.4
28.6
40.6
23.6
25.9
1130
1110
262
5470
4290
5330
2830
5596
6782
1584
27514
10823
13449
22284
A-128
-------
Table A-56. Continued.
Station
E
Comp A
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Laboratory
Bat A
Bat A
Bat B
Bat C
Bat D
BatE
Bat F
Bat G
Lipid 13Ce-TeCB
Percent % Recovery
1.26 39.5
31.4
23.6
27.8
23.5
27.8
29.1
28.9
Corrected
[ng/g]
2870
11.5
1.50
2.11
0.00
6.30
2.23
0.840
Lipid
Corrected
17290
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
A-129
-------
Table A-57. 1,2,3-Trichlorobenzene Tissue Concentrations for Brevoortia patronus and
Micropoaan undulus.
Concentration
Concentration (Blank Corrected,
13Ca-TeCB (Recovery 7.6% Lipid
Lipid Recovery Corrected) Content)
Station Laboratory* % % [ng/g] [ng/g]
Brevoortia patronus
c
Comp A
Comp B
Comp C
Comp D
Comp E
Comp F
Comp G
Comp G
D
Comp A
Comp B
E
Comp A
Comp B
Comp B
Comp C
Comp D
Bat A
Bat A
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat BCD
2.77
2.56
3.46
6.15
3.28
3.22
3.14
2.34
3.22
2.06
3.05
2.37
1.54
37.1
28.0
25.3
48.2
39.0
39.1
21.2
46.5
27.8
30.0
22.0
25.4
23.6
21.8
24.4
MicroDoaan undulus
B
Comp A
C
Comp A
Comp A
D
Comp A
Comp B
Comp B
Comp C
Bat A
Bat BCD
Bat BCD
Bat A
Bat A
Bat A
Bat BCD
1.53
1.24
1.51
3.01
0.964
37.1
41.7
17.4
28.6
40.6
23.6
25.9
31.1
59.7
33.2
87.0
59.2
52.6
58.3
48.4
73.3
45.1
60.1
65.6
63.7
83.6
38.3
19.0
10.8
3.21
66.1
54.6
68.8
23.2
78.2
170
67.2
104
131
118
135
111
230
100
212
157
152
260
176
81.4
50.2
3.72
320
131
167
162
A-130
-------
Table A-57. Continued.
Station
E
Comp A
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Laboratory'
Bat A
Bat A
Bat B
BatC
Bat D
Bat E
Bat F
Bat G
13Ca-TeCB
Lipid Recovery
% %
1.26 39.5
31.4
23.6
27.8
23.5
27.8
29.1
28.9
Concentration
(Recovery
Corrected)
[ng/g]
38.7
4.58
0.00
0.00
3.42
4.57
2.21
3.44
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
218
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
A-131
-------
Table A-58. 1,2,4-Trichlorobenzene Tissue Concentrations for Brevoortia patronu$ and
Micropoaan undulus.
Station Laboratory"
13Ca-TeCB
Lipid Recovery
Concentration
Concentration (Blank Corrected,
(Recovery 7.6% Lipid
Corrected) Content)
[ng/g] (ng/gl
Brevoortia oatronus
c
Comp A
Comp B
Comp C
Comp D
Comp E
Comp F
Comp G
Comp G
D
Comp A
Comp B
E
Comp A
Comp B
Comp B
Comp C
Comp D.
Bat A
Bat A
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat BCD
2.77
2.56
3.46
6.15
3.28
3.22
3.14
2.34
3.22
2.06
3.05
2.37
1.54
37.1
28.0
25.3
48.2
39.0
39.1
21.2
46.5
27.8
30.0
22.0
25.4
23,6
21.8
24.4
130
339
133
565
383
327
297
275
504
283
374
393
365
489
192
Micropoqan pndulus
B
Comp A
C
Comp A
Comp A
D
Comp A
Comp B
Comp B
Comp C
Bat A
Bat BCD
Bat BCD
1.53
1.24
37.1
41.7
17,4
353
1002
289
696
884
768
715
662
1632
665
1374
976
906
1563
940
73.1
39.3
9.85
Bat A
Bat A
Bat A
Bat BCD
1.51
3.01
0.964
28.6
40.6
23.6
25.9
286
336
455
133
356
232
51.4
1432
845
1145
1037
A-132
-------
Table A-58. Continued
Station
E
Comp A
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Laboratory'
Bat A
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
13C6-TeCB
Lipid Recovery
% %
1.26 39.5
31.4
23.6
27.8
23.5
27.8
29.1
28.9
Concentration
(Recovery
Corrected)
[ng/g]
215
1.90
0.00
0.00
1.02
1.05
3.11
3.12
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
1288
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
A-133
-------
Table A-59. 1,2,4,5- and 1,2,3-5 Tetrachlorobenzene Tissue Concentrations for Brevoortia
oatronus and Micropoaan undulus.
Concentration
Concentration (Blank Corrected,
13Cfl-TeCB (Recovery 7.6% Lipid
Recovery Corrected) Content)
% tng/g] (ng/g]
Station
Laboratory*
Lipid
%
Brevoortia oatronus
c
Comp A
Comp B
Comp C
Comp D
Comp E
Comp F
Comp G
Comp G
D
Comp A
Comp B
E
Comp A
Comp B
Comp B
Comp C
Comp D
Bat A
Bat A
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat BCD
2.77
2.56
3.46
6.15
3.28
3.22
3.14
2.34
3.22
2.06
3.05
2.37
1.54
37.1
28.0
25.3
48.2
39.0
39.1
21.2
46.5
27.8
30.0
22.0
25.4
23.6
21.8
24.4
117
227
129
343
274
278
221
200
350
252
295
261
234
392
123
Microppqan undulus
B
Comp A
C
Comp A
Comp A
D
Comp A
Comp B
Comp B
Comp C
Bat A
Bat BCD
Bat BCD
1.53
1.24
17.4
37.1
41.7
47.1
317
670
280
422
632
653
532
481
1132
592
1083
647
580
1253
600
63.5
36.5
280
Bat A
Bat A
Bat A
Bat BCD
1.51
3.01
0.964
28.6
40.6
23.6
25.9
216
222
268
94.4
309
216
1080
557
673
734
A-134
-------
Table A-59. Continued.
"
Station
E
Comp A
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Laboratory'
Bat A
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
BatG
13C8-TeCB
Lipid Recovery
% %
1.26 39.5
31.4
23.6
27.8
23.5
27.8
29.1
28.9
Concentration
(Recovery
Corrected)
[ng/g]
132
1.86
3.36
1.03
0.958
0.00
1.39
0.778
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
788
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
A-135
-------
Table A-60. 1,2,3,4-Tetrachlorobenzene Tissue Concentrations for Brevoortia patronus
and Micropoaan undulus.
Concentration
Concentration (Blank Corrected,
13Ca-TeCB (Recovery 7.6% Lipid
Lipid Recovery Corrected) Content)
Station Laboratory* % % [ng/g] [ng/g]
Brevoortia oatronus
c
Comp A
Comp B
Comp C
Comp D
Comp E
Comp F
Comp G
Comp G
D
Comp A
Comp B
E
Comp A
Comp B
Comp B
Comp C
Comp D-
Bat A
Bat A
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat BCD
2.77
2.56
3.46
6.15
3.28
3.22
3.14
2.34
3.22
2.06
3.05
2.37
1.54
37.1
28.0
25.3
48.2
39.0
39.1
21.2
46.5
27.8
30.0
22.0
25.4
23.6
21.8
24.4
143
343
155
423
352
348
276
243
456
283
391
345
300
514
159
Micropoaan undulus
B
Comp A
C
Comp A
Comp A
D
Comp A
Comp B
Comp B
Comp C
Bat A
Bat BCD
Bat BCD
1.53
1.24
37.1
41.7
17.4
390
1016
339
522
814
819
666
586
1478
666
1439
857
745
1645
780
69.1
41.2
59.6
Bat A
Bat A
Bat A
Bat BCD
1.51
3.01
0.964
28.6
40.6
23.6
25.9
275
266
333
119
339
247
360
1380
669
839
931
A-136
-------
Table A-60. Continued.
Concentration
Concentration (Blank Corrected,
Station
E
Comp A
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Laboratory"
Bat A
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
13C6-TeCB
Lipid Recovery
% %
1.26 39.5
31.4
23.6
27.8
23.5
27.8
29.1
28.9
(Recovery
Corrected)
[ng/g]
158
0.954
0.00
1.57
1.73
0.00
1.49
0.467
7.6% Lipid
Content)
[ng/g]
948
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
A-137
-------
Table A-61. Pentachlorobenzene Tissue Concentrations for Brevoortia patronus and
Microppqan undulus.
Concentration
Concentration (Blank Corrected,
13C0-HCB (Recovery 7.6% Lipid
Recovery Corrected) Content)
% [ng/g] [ng/g]
Station
Laboratory"
Lipid
%
Brevoortia oatronus
c
Comp A
Comp B
Comp C
Comp D
Comp E
Comp F-
Comp G
Comp G
D
Comp A
Comp B
E
Comp A
Comp B
Comp B
Comp C
Comp D
Bat A
Bat A
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat BCD
2.77
2.56
3.46
6.15
3.28
3.22
3.14
2.34
3.22
2.06
3.05
2.37
1.54
32.2
23.1
24.6
36.7
33.0
31.6
19.4
39.4
25.6
27.7
18.2
21.9
21.3
20.3
23.7
778
1570
753
1740
1510
1763
1260
1250
1910
1730
2240
1330
1240
2160
594
Micropoaan undulus
B
Comp A
C
Comp A
Comp A
D
Comp A
Comp B
Comp B
Comp C
Bat A
Bat BCD
Bat BCD
1.53
1.24
31.8
45.8
29.8
399
268
331
Bat A
Bat A
Bat A
Bat BCD
1.51
3.01
0.964
39.3
31.9
27.0
29.5
910
1110
1140
532
2129
4655
1649
2148
3494
4156
3045
3021
6197
4078
8257
3309
3085
6920
2921
1972
1630
2016
4570
2797
2873
4178
A-138
-------
Table A-61. Continued.
*
Station
E
Comp A
Blank
Blank
Blank
Blank
Blank
Blank
Blank
Lipid
Laboratory" %
Bat A 1 .26
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
13C8-HCB
Recovery
%
35.1
36.9
32.2
29.5
24.6
37.6
35.1
34.0
Concentration
(Recovery
Corrected)
[ng/g]
560
2.47
3.74
0.967
1.38
1.35
3.63
0.779
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
3365
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
A-139
-------
Table A-62. Hexachlorobenzene Tissue Concentrations for Brevoortia patronus and
Micropooan undulus.
Concentration
Concentration (Blank Corrected,
13C8-HCB (Recovery 7.6% Lipid
Recovery Corrected) Content)
% (ng/gl [ng/g]
Station Laboratory*
Lipid
%
Brevoortia patronus
c
Comp A
Comp B
Comp C
Comp D
Comp E
Comp F
Comp G
Comp G
D
Comp A
Comp B
E
Comp A
Comp B
Comp B
Comp C
Comp D-
Bat A
Bat A
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat A
Bat A
Bat BCD
Bat BCD
Bat BCD
Bat BCD
Bat BCD
2.77
2.56
3.46
6.15
3.28
3.22
3.14
2.34
3.22
2.06
3.05
2.37
1.54
32.2
23.1
24.6
36.7
33.0
31.6
19.4
39.4
25.6
27.7
18.2
21.9
21.3
20.3
23.7
615
912
632
1030
894
1020
920
877
1230
1290
3140
860
863
1240
410
1679
2698
1381
1269
2064
2400
2219
2115
3985
3037
11573
2135
2143
3966
2008
Micropoaan undulus
B
Comp A
C
Comp A
Comp A
D
Comp A
Comp B
Comp B
Comp C
Bat A
Bat BCD
Bat BCD
1.53
1.24
31.8
45.8
29.8
447
282
410
Bat A
Bat A
Bat A
Bat BCD
1.51
3.01
0.964
39.3
31.9
27.0
29.5
729
724
912
377
2205
1709
2494
3653
1820
2295
2948
A-140
-------
Table A-62. Continued.
Station
E
Comp A
Blank
Blank
Blank
Blank
Blank
Blank .
Blank
Laboratory8
Bat A
Bat A
Bat B
Bat C
Bat D
Bat E
Bat F
Bat G
13Ce-HCB
Lipid Recovery
% %
1.26 35.1
36.9
32.2
29.5
24.6
37.6
35.1
34.0
Concentration
(Recovery
Corrected)
[ng/g]
418
3.78
1.33
6.06
3.27
2.74
2.43
2.25
Concentration
(Blank Corrected,
7.6% Lipid
Content)
[ng/g]
2502
Bat: Battelle-Columbus, ERL-D: Environmental Research Laboratory-Duluth. The
letters or date following the laboratory abbreviation are the procedural blanks done
with the analysis of the sample.
A-141
-------
v>EPA
United States ,
Environmental Protection
Agency
EN-336
Washington, DC 20460
Official Business
Penalty for Private USB
$300
SPECIAL 4TH CLASS
BOOK RATE
Postage and Fees Paid
EPA
G-35
------- |