SEPA
United States      Industrial Environmental Research  EPA-600/7-79-251a
Environmental Protection  Laboratory          November 1979
Agency        Research Triangle Park NC 27711
Effects of Pathogenic
and Toxic Materials
Transported Via Cooling
Device Drift -
Volume 1. Technical Report

Interagency
Energy/Environment
R&D  Program  Report

-------
                  RESEARCH REPORTING  SERIES


Research reports of the Office of Research and Development, U.S. Environmental
Protection Agency, have been grouped into nine series. These nine broad cate-
gories were established to facilitate further development and application of en-
vironmental technology. Elimination of traditional grouping was  consciously
planned to foster technology transfer and a maximum interface in related fields.
The nine series are:

    1. Environmental Health Effects Research

    2. Environmental Protection Technology

    3. Ecological Research

    4. Environmental Monitoring

    5. Socioeconomic Environmental Studies

    6. Scientific and Technical Assessment Reports (STAR)

    7. Interagency Energy-Environment Research and Development

    8. "Special" Reports

    9. Miscellaneous Reports

This report has been  assigned to the INTERAGENCY ENERGY-ENVIRONMENT
RESEARCH AND DEVELOPMENT series. Reports in this series result from the
effort funded under the 17-agency Federal  Energy/Environment Research and
Development Program. These studies relate to EPA's mission to protect the public
health and welfare from adverse effects of pollutants associated with energy sys-
tems. The goal of the Program is to assure the rapid development of domestic
energy supplies in an environmentally-compatible manner by providing the nec-
essary environmental data and control technology. Investigations include analy-
ses of the transport of energy-related pollutants and their health and ecological
effects;  assessments  of, and development of,  control  technologies for energy
systems; and integrated assessments of a wide'range of energy-related environ-
mental issues.
                        EPA REVIEW NOTICE
This report has been reviewed by the participating Federal Agencies, and approved
for  publication. Approval does not signify that the contents necessarily reflect
the  views and policies of the Government, nor does mention of trade names or
commercial products  constitute endorsement or  recommendation for use.

This document is available to the public through the National Technical Informa-
tion Service, Springfield, Virginia 22161.

-------
                                         EPA-600/7-79-251a

                                              November 1979
Effects of Pathogenic and Toxic Materials
  Transported Via Cooling Device Drift  -
           Volume  1. Technical Report
                             by

                 H.D. Freudenthal, J.E. Rubinstein, and A. Uzzo

                         H2M Corporation
                         375 Fulton Street
                     Farmingdale, New York 11735


                       Contract No. 68-02-2625
                     Program Element No. INE624A
                   EPA Project Officer: Michael C. Osborne

                 Industrial Environmental Research Laboratory
               Office of Environmental Engineering and Technology
                    Research Triangle Park, NC 27711
                           Prepared for

                 U.S. ENVIRONMENTAL PROTECTION AGENCY
                    Office of Research and Development
                       Washington, DC 20460

-------
                                ABSTRACT
     The report describes a mathematical model that predicts the percent
of the population affected by a pathogen or toxic substance emitted in a
cooling tower plume, and gives specific applications of the model.
Eighty-five pathogens (or diseases) are cataloged as potentially
occurring in U.S. waters, but there is insufficient data to predict the
probability of occurrence or relate their occurrence to public health,
population, or pollution.  Sixty-five toxic substances are cataloged as
potentially occurring in U.S.  waters, but the actual number is probably
many times the EPA-supplied list.  Toxic concentrations to persons,
animals, and plants are known for only a few of the chemicals:  most
toxic levels can be only inferred from animal studies.  In the popula-
tion as a whole, the epidemiological impact of a pathogen is a functio.n
of age, sex distribution, racial (genetic) distribution, general health
and well-being, prior exposure, and immunological deficiency states.
While cooling device drift may not be directly responsible for epidemics,
it may potentiate the burden in an already weakened population, raising
a segment of the population into the clinical state.  The effect of
toxic substances is difficult to evaluate because of inadequate data on
humans.  The effect is a function of concentration in susceptible tissue,
and is much less dependent than pathogens on host resistance.

-------
                            CONTENTS
Abstract	 i i
Figures	 iv
Tables	  v
Abbreviations and Symbols	vii

   1.  Introduction	  1
   2.  Conclusions	  4
   3.  Recommendations	 11
   4.  Objectives	 14
   5.  Methodology	 24
   6.  Results
           Task I - Inventory	 27
           Task II - Transport	 50
           Task III - Direct Effects	 93
           Task IV - Indirect Effects	 99
           Task V - Recommendations	101
           Task VI - Computer Simulation	102

References	176
Bibliography	180
Appendices

       Introduction to Catalog	 iii
   A.  Aerosol Drift Health Hazard Assessment	A-l
   B.  Aerosol Drift Direct Effects Assessment	B-l
                              111

-------
                              FIGURES

  Number                                                       Page

    1  Information flow diagram	 15

    2  Task assignment diagram	 26

    3  Typical natural and mechanical draft cooling towers	 52

    4  Fossil fuel steam electric plant using hyperbolic towers
          has a heat balance as shown for 1000-MW capacity	 54

    5  Nuclear steam electric plant using hyperbolic tower has
          a heat balance as shown for 1000-MW capacity	 55

    6  Percent by number vs. droplet size	 59

    7  Fall velocity of water drops as function of size	 62

    8  Dispersion of water drops as function of size	 63

    9  Mass size distribution percentage of total mass less     65
          than stated	

   10  Calibration factor vs. downwind distance for examples
          of Natural (N) and Mechanical (M) draft cooling
          tower drift deposition	 80

   11  Basic flow chart	102

   12  Microbial content of cooling tower	113

   13  Droplet size distribution	115

   14  Spatial organization of organisms	117

   15  Infectivity model	119

   16  Typical data file	121

17-21  Sample runs of stochastic model	122

   22  Simulation program listing	168
                                IV

-------
                             TABLES

Number                                                       Page

  1  Types of Cooling Towers	 18

  2  Pathogens Most Likely to Occur in Cooling Tower
        Makeup Water Sources	 30

  3  Toxic Substances Potentially Present in Cooling
        Makeup Water	 37

  4  Attenuation of Pathogens and Status in Makeup Water	 41

  5  Attenuation of Toxic Substances and Status in Makeup
        Water	 45

  6  Pathogens Potentially Present Only in a Worst Case
        Situation	 48

  7  Screened Pathogens Capable of Becoming Aerosolized	 49

  8  Toxins Capable of Becoming Aerosolized	 50

  9  Common Water Treatment Chemicals	 57

 10  Characterization of Condensation Nuclei	 67

 11  Model Input Parameters for Calculation of Table 12	 75

 12  Typical Ground Level Distribution of Drift Particles
        From Natural Draft Cooling Towers	 76

 13  Model Input Parameters for Calculation of Table A	 78

 14  Example of Ground Level Distribution of Drift Particles
        From a Mechanical Draft Cooling Tower	 79

 15  Organisms Reviewed for Aerosol Survival and
        Transmissibility	 82

 16  Kill Percentage of Viruses Exposed to Ultra Violet
        Radiation	 87

 17  Events Influencing Microbiological Survival	 88

 18  Numerical Constants Used in Estimating Probabilities.... 104

-------
                          TABLES CONT.






Number                                                       Page



  19  Summary of Pathogen/Toxin Probabilities	 105



  20  Typical Input Parameters	 121
                               VI

-------
                LIST OF ABBREVIATIONS AND SYMBOLS
BTU           British Thermal Unit
BW            Biological Warfare
Ca            Calcium
Cl            Chlorine
cm            centimeter
DAT           Dynamic Aerosol Toroid
EMV           Encephalomyocarditis Virus
EST           Eastern Standard Time
FD            Forced Draft Cooling Device
Fe            Iron
g             grams
gpm           gallons per minute
HCO^          bicarbonate ion
ID            Induced Draft Cooling Device
K             Kelvin
Km            Kilometer
1             liter
L/G           Water rate/air rate (Ibs/time)
m             meter
m3            cubic meter
mb            millibar
mm            millimeter
mg            milligrams
mw            megawatt
Na            Sodium
0             Oxygen
(OH)~         Hydroxide ion
ppm           parts per million
r             radius
RH            Relative Humidity
SO^~          Sulfate
SV4Q          Simian Virus 40
Teo           ambient temperature near exit from tower
Tpo           plume temperature
u             micron
VSV           Vesicular Stomatitis virus
WB            Wet Bulb
                               VII

-------
                           SECTION 1
                         INTRODUCTION
     The recent trend in the electric power generating industry
and major industries has been the use of closed circuit cooling
devices.  The use of these devices supercedes the use of once-
through cooling systems which had the disadvantage of harmful
thermal discharges into surface waters and fish impingement or
entrainment.  However, the closed circuit systems, or cooling
towers, have potential problems, too.

     Previous studies have shown that a potential health hazard
could exist if pathogens were to be dispersed in cooling tower
drift (Lewis, 1974; Cummings, 1964; Dvorn and Wilcox, 1972).  This
could occur as a result of drawing make-up water from highly pol-
luted surface waters or from the use of processed wastewater
(reclaimed water) with inadequate microbial control.  In addition
to the direct effects on plants such aerosols might potentially
cause severe environmental damage, and present legal difficulties
to the source operator, especially if the aerosol source impacted
dense population centers.

     Two prior studies were conducted by H2M for the Consolidated
Edison Company of New York (Con Ed) and for a midwest utility
that prefers to remain anonymous.  Under the provisions of the
work statement for the Con Ed study, H2M was to:

     a.  Inventory the sources of possible pathogen pollution
         in the Hudson, from the Bronx to Albany.

     b.  Prepare a catalogue of all possible organisms which could
         reasonably occur in the area and be transported by the
         cooling tower drift route.

     c.  Estimate the magnitude of the severity of the problem.

     d.  Describe the water treatment methods which would be
         needed to provide positive control over pathogens and
         totally eliminate the problem.

     The one month study, based entirely upon published data and
no field observations, drew the following conclusions:

-------
      a.   Based upon  coliform bacterial levels, used as  indicators
          of  fecal  pollution,  the  contamination of the Hudson  is
          sufficiently  high  to present the possibility of the
          occurrence  of pathogens.

      b.   The physical  conditions  of temperature, pressure and
          flow in the cooling tower circuit will not attenuate
          pathogens,  and during some months, may even prolong
          survival.   The biocides  which are added for algae con-
          trol are  ineffective against many pathogens (viruses,
          spores, etc.).

      c.   Many pathogens will survive aerosolization and can be
          transported in a virulent condition over thousands of
          square miles.

      On the  basis  of the findings of this early study,  it was
concluded that the possibility of disease transmission through
cooling tower drift  exists.   It was also concluded that the proba-
bility of this happening is  very  low.  However, it is remotely
possible  that the proper combination of a badly contaminated slug
of water, inadequate biocidal treatment,  plus unfavorable atmo-
spheric conditions could disperse millions of virulent organisms.
Even  if not  particularly virulent, the constant loading of the
atmosphere with biological and chemical respiratory offenders pre-
sented a  potential of  a general increase in "colds" and allergies,
contributing  to the  overall  discomfort and loss of productivity
of populated  areas.

     That study did  not  produce firm recommendations due to the
inadequacy of  factual  information.  It did however conclude that
the problem  is sufficiently  critical to warrant further study.

     A study  performed  by NUS  in 1974 for Public Service Gas and
Electric  Company of  New Jersey, on the potential virus hazard from
their Bergen, Burlington and  Mercer plants concluded that there
would be  no hazard.  But the  authors neither sampled virus from
the rivers of the respective  plants,  nor did they discuss any
literature addressing  specific organisms.   Meanwhile,  a very com-
prehensive review of the "health significance of airborne micro-
organisms from wastewater treatment processes," by Hickey and
Reist (1975)  stated:

     "The body of evidence is  persuasive that some as yet unde-
      termined health  effects  occur from viable wastewater aerosols."

     This premise was  confirmed by Walka (1976) in a thesis on the
distribution  of bacterial aerosols from a sewage treatment plant.
He concluded  that a  survey is  needed,  especially examining the
epidemiological effect  of aerosols on populations surrounding
wastewater treatment facilities.   It was not felt that additional
microbial monitoring around aeration tanks would be productive.

-------
     Work currently in progress by Lewis and Adams (1978) includes
development of a sampling program seeking opportunistic bacteria,
indicated by coliforms, in cooling device drift.  Sampling was per-
formed at five sites, drawing make up water from a variety of pol-
luted sources.  One completed study examines asbestos in cooling
waters and a subsequent study is planned to sample for asbestos
in the ambient environment.

     Work has proceeded in examining the chemistry and effects of
biocides in cooling towers (Jolley, 1977),  and on the health of
humans and aquatic organisms.  The Electric Power Research Institute
(EPRI) has acknowledged the need for further research and has been
looking into the state of the art.

-------
                           SECTION 2

                          CONCLUSIONS

     In summary, the following conclusions may be drawn from this
study.

I.    OCCURRENCE

1.    Pathogens Potentially Present

     Eighty-five (85) pathogens (or diseases) have been catalogued
as potentially occurring in United States waters.  There is insuf-
ficient data to predict the probability of occurrence or relate
their occurrence to public health, population, or pollution.  The
coliform test has no proven relationship between the occurrence
of coliforms and specific pathogens,  except for the one case of
Salmonellae in which the frequency of occurrence varied directly
with coliform values, (Geldreich and Van Donsel,  1970).  It must
be assumed that polluted water or sanitary effluent may carry
pathogenic viruses, bacteria,  fungi,  protozoa, or helminths, either
as indigenous organisms or introduced through any of several natural
or anthropogenci routes.


2.   Toxic Substances Potentially Present

     Sixty-five (65) toxic substances have been catalogued  as
potentially occurring in United States waters, although the  actual
number is probably many times this EPA-supplied list.  The  occurrence
of specific substances  has been definitely related to land  usage.
Toxic concentrations to man, other animals and plants are known
for only a few of the chemicals, for most toxic levels can  be only
inferred from animal studies.  Concentrations in natural waters or
wastewater effluent are not well documented, but toxic levels have
been reported in many parts of the country.  Although it is a
natural goal to remove  these materials from the environment,
it must be assumed that they may be present.  It must also  be
assumed that these substances are capable of producing clinical
or sub-clinical toxic reactions in humans and other living
organisms, even though  the relationship has not always been demon-
strated.

3.   Use of Polluted Natural and Waste Waters as Make-Up Water

     If cooling devices draw make-up water from polluted natural

-------
waters or wastewater with less than total purification,  it must
therefore be assumed that microorganisms and toxic substances
capable of producing disease may be incorporated into the circula-
ting water of the device.  As major cooling devices withdraw large
quantities of water to replace that lost by evaporation  (e.g.
16 mgd for a 1,000 megawatt fossil fuel power plant), the probability
of taking up pathogens or chemical substances is great if these
occur in the source of make-up water.


II.  SURVIVAL IN THE COOLING DEVICE

1.   Pathogens

     Most cooling devices have a mean  circulating water  temperature
close to human body temperature and the temperature which favors
the growth of mammalian pathogens.  Therefore, in the absence of
biocides, the microorganism will survive and may multiply if
suitable nutrients are present.  Biocides, of the type usually
used to control algal growth, may have limited efficiency in destroy-
ing, or attenuating pathogens, and even the strongest of commercial
disinfectants may have little effect on viruses.  The removal
of some of the circulating water through  "blow-down" may
establish a steady state population of viable organisms  in the
circulating water.

2.   Toxic Substances

     Dissolved or suspended toxic substances will not normally
be attenuated in circulating water within the cooling device.
Pretreatment of make-up water, or the  use of water "conditioners"
may precipitate-out or otherwise attenuate the concentration
of these substances.  Removal of the substances in the "blow-
down" may establish a steady state concentration.

3.   Survival and Persistence

     It must be assumed that pathogens and toxic substances
will survive and persist within the cooling device environment.


III. CONVERSION INTO DRIFT

1.   Droplet Size and Composition

     As drift is produced as droplets  of water, the size of the
droplets is adequate to contain almost all pathogenic microorganisms
and dissolved or suspended toxic materials.  The drift will have
essentially the same chemical and- biological composition as the
circulating water.

-------
 2.    Quantity of Loss through Drift

      Although the loss of water as drift  is  a  small  percentage
 of  the circulating water, the quantities  are still  large in major
 cooling devices.  (e.g.  a 1,000 megawatt  fossil  fuel power plant
 may release 13 mgd of water as drift,  equivalent to  the water
 consumption of a city of 250,000 people.)

 3.    Passage of Pathogens and Toxic  Substances into  Aerosol State

      The potential is therefore great  for  the  passage of substan-
 tial quantities of pathogens and toxins into drift,  if these are
 present in the circulating water.


 IV.   TRANSPORT IN AEROSOL DRIFT

 1.    Deposition of Drift

      A substantial portion of the drift will be  deposited in the
 vicinity of the cooling  device, as demonstrated  in  drift models
 and studies with drift generating devices.   However,  meteorological
 phenomena could incorporate the drift  into strong surface winds,
 clouds, or the upper atmosphere,  resulting in  transport over
 hundreds of miles.

2_.	Pathogen Transport  in Aerosol Drift

      Pathogens are of such size that they  are  capable of being
 transported in the drift or atmosphere for substantial distances.
 This transport has been  documented.

3_.	Toxin Transport in Aerosol Drift

      Toxic substances will behave as the water droplets in the drift,


 V.    SURVIVAL IN AEROSOL DRIFT

 1.    Pathogen Survival in Aerosol Drift

      Microorganisms in drift are normally  attenuated by dessication,
 temperature and ultraviolet radiation.  A  drift  produced in an
 arid climate during the  day would show a great reduction of some
 pathogens, principally bacteria and  protozoa.   A drift produced
 in  a humid atmosphere, at cold temperatures, and at  night would
 have little attenuation.   Drift droplets that  freeze would insure
 survival of almost  all pathogens.  Dense cloud cover,  or the density
 of  the plume itself,  would restrict  UV penetration,  and would reduce
 attenuation.

 2.    Toxic Substance Integrity in Aerosol  Drift

                                 6

-------
     Few of the toxic substances considered were sensitive to
light or moisture.  Attenuation in the drift would be negligi-
ble.  Even if the water of the drift evaporates, most substances
would be suspended as gas, aerosols or particles, without
modification.  In some cases, toxic materials could undergo
photochemical reactions, increasing their toxicity.

3.   Effect of Ambient Environment on Survival and Integrity

     It must be assumed that drift can transport pathogens and
toxins without significant attenuation.  Ambient site charac-
teristics influence the viability of some microorganisms, but
have little effect on toxic molecules as a whole.
VI.   PRODUCTION OF DISEASE OR CLINICAL MANIFESTATIONS

1.   Human Susceptibility

Pathogens
     The pathogens' ability to produce disease or clinical
manifestations is a function of arrival of a sufficient number of
infective particles at a suitable portal of entry and the suscep-
tibility of the host.  Bacterial infectivity is a function of
a stoichiometric relationship between the number of organisms
and hosts' antibodies, and the number required  may vary from a
few to several hundred thousand.  For viruses, protozoa, fungi,
and worms, the number of infective particles may be as few as one,
As the half life of pathogens in the environment may be long,
accumulation or continuous exposure could bring the number of
particles to critical levels.

     In the population as a whole, the epidemiological impact
is a function of age, sex distribution, racial (genetic) distri-
bution, general health and well-being, prior exposure and immuno-
logical deficiency states.  While cooling device drift itself may
not be directly responsible for epidemics, it may potentiate
the burden in an already weakened population, raising a segment
of the population into the clinical state.

Toxic Substances
     The effect of toxic substances is difficult to evaluate
because of inadequate data on humans.  The effect is a function
of concentration in susceptible tissue, and is much less depen-
dent on host resistance than for pathogens.  Immunity can not be
acquired either.
     Death directly due to most drift borne toxic substances
is unlikely, based upon the concentrations implied by the
limited data.  Cancers are most likely, but the data is also
insufficient to draw any conclusions.  A highly probable but
speculative impact is the weakening of individuals, making them
susceptible to infection, or allergic reactions.

-------
2.   Animal Susceptibility

Pathogens
     The general pathogen considerations for animals are the same
as for humans, except that herbivores graze directly on vegetation
and therefore have a greater potential for accumulating infectious
particles from plants exposed to drift.

Toxic Substances
     The accumulation of toxic substances on vegetation presents
a greater probability of accumulation to toxic levels within grazing
herbivores.

3.   Vegetation Susceptibility

Pathogens
     Only the fungi and viruses are significant as pathogens,
and the usual route of transfer is by vectors or dry wind.  Cooling
device drift is not a significant factor.

Toxic Substances
     The effect of the toxic substances considered is not well
documented,  but drift transport does not appear to be a significant
factor.  The water droplets themselves, humidity, or ordinary salts
in the drift are documented as causes of plant disease.


VII.  PROBABILITY OF OCCURRENCE

     In determining the relative probability of host contamination,
a number of  assumptions and parameters had to be worked into the
mathematical model.  These specifically include:

     a.  Cooling tower height:           400 ft.

     b.  Top diameter:                  300 ft.

     c.  Exit air volume:                23 x 10® cubic feet per min,

     d.  Evaporative loss:               13 cubic feet per second

     e.  Aerosol loss:                  0.01 cubic feet per second

     f.  Wind Speed:                     30 feet per second

     g.  Air temperature:                300  K.

     h.  Relative humidity:              70%

     i.  Circulating water volume:       5 x 10® cubic feet

     j.  Blow-down: Complete blow-down @ 1.5:1 concentration ratio

                               8

-------
     Other parameters which were selected for inclusion  in the
model were based on typical power plant load profiles and
weather conditions for 24 hour periods.  Each 24 hour period
was divided into four hour segments and it was assumed that
conditions remained constant over this period.  The numerical
values which were chosen and integrated into the model were
considered typical for a natural draft cooling tower for a
plant of approximately 1000 MW capacity.  These parameters
include drift fraction e.g. 5x10 ~5 gig, salt concentration
ratios of 30%, weather conditions for typical seasonal,  day
and evening instances, plant operating capacity and atmospheric
stability.  The specific values assigned to the parameters are
detailed on the printout of each case.

     A calculation was then performed to determine the proba-
bility of contamination using those assumptions and parameters.
The following is a sample of the results achieved for a  summer's
day case.
      	   24 HOUR TOTALS  	

      DAILY PROBABILITY OF EFFLUENT CONTAINING ORGANISMS .34

      DISK MI)        QRG/M3/DAY       ORG/M2/DAY
          0. 10               0. 0              0. 0
          0.15         1107339.0         454345.4
          0.20          311119.8         129253.4
          0,30           98662.0          41190.6
          0,50          105227.0         232410.3
          0.75          215556.6         369901.7
          1.00          280V64.0         379159.9
          1,50          155911.4         141004.1
          2.00           94571.7          58955.1
          2,50           66768.6          33844.5
          3,00           50172.2          21953.7
          4.00           17523.1           5660.8
          5-00           12931.0           3511.6
          7.00            8573.2           1983.8
          9,00            6487.0           1202.0
         10.00            5151.9            756.5
         12.OO            4226.6            604.1
         15.00            1685.0            230.4
         20.00             986.8            107.6
         25.00             664.9             54.7
                              9

-------
              SUMMARY  OF  RESULTS
DIST(MI)
   0. 10
   0. 15
   0.20
   0.30
   O.50
   0.75
     , 00
     , 30
     oo
     ,50
 1,
 1.
 2.
 2.
 3.00
 4.00
 5.0U
 7.00
 9.00
10.00
12.00
15.00
20.00
25.00
AVG NO. PART.
  INGESTED/IND.

         0.0
   1561684.4
    440373.2
    139852.6
    337637.3
    585458.3
    660123.9
    296915.5
    153526.8
    100613.1
     72125.9
     23183.9
     16442.5
     10557.1
      7689.0
      5908.4
      4830.7
      1915.4
      1094.5
       719.6
PERCENT AFFECTED
     BY EFFLUENT

          O.OOO
         18.135
          0.486
          1.223
         20.0OO
          9.244
         13.324
         18.221
         11.626
         10.064
          5.507
          0.486
          1.914
          0.318
          O.OO6
          O.OOO .
          0.000
          O.OOO
          0.409
          0.215
                        10

-------
                           SECTION 3
                        RECOMMENDATIONS


I.   General Epidemiology and Microbiology

     As in all public health and water supply work, the confi-
dence in health hazard projections is low because of inadequate
data.  To compensate for the inability to make accurate projec-
tions, the United States had adopted a technology of extra caution
in water treatment.  While this has brought this nation an absence
of infectious disease heretofore unknown in the history of man-
kind, it has imparted cost, energy and environmental penalties,
as well as possible health hazards of the non-infectious type.

     A.  Society must establish the level of public health
         that it is willing to accept,  together with the
         economic and social cost.  This is a political de-
         cision beyond the scope of cooling devices alone,
         but it is incumbent upon science and engineering to
         develop the costs and benefits of alternative tech-
         nologies.  Studies to develop these, and measure
         public attitudes, should be performed.

     B.  The use of the coliform test as an indicator of
         health hazard is a poor substitute for actual pathogen
         monitoring, justifiable only when no alternative methods
         were feasible.  Today, equipment and procedures exist
         for rapidly identifying specific pathogens.  The advances
         of space technology and diagnostic medical microbiology
         should be applied to water monitoring, for cooling
         devices and all other water use sciences.

     C.  Aerobiological and aerochemical studies should be performed
         in the United States and Europe to determine if the
         microbiological and chemical load imposed upon the
         atmosphere by cooling device drift is so significantly
         above background as to constitute a possible health hazard.
         Studies using "tagged" organisms and chemicals should
         be considered in addition to environmental monitoring.

     D.   Epidemiological studies should be conducted in areas in
         which major cooling devices have been in use,  especially
         those which use polluted water for make-up.  The selec-
         tion of sites is very critical.  They must have adequate
         health data prior to the use of the cooling device, and

                                11

-------
         the general level of health should be good.  Such study
         sites may be found in Europe.

     E.  Laboratory models should be constructed, using polluted
         water, cooling device drift simulation, and animals, to
         experimentally derive data on  health impact.

     F.  Special attention should be given to subclinical and
         allergic manifestations of infection and toxicity pro-
         duced by cooling device drift  and other sources of air
         pollution.  Current health data suggest an increase of
         health problems directly related to environmental pollu-
         tion.  Those which result in clinical symptoms are easi-
         est to document, however, there appears to be an increase
         in those conditions which cause discomfort, decrease
         resistance to infectious disease, initiate autoimmune
         "cancerous" conditions, and generally shorten life or
         decrease productivity.  These  are typified by allergies,
         "colds," etc., i.e. those conditions which do not call
         for a medical practitioner, but are nevertheless debili-
         tating or unrecognizable.

II.  Cooling Device Technology

     A.  In as much as cooling devices  may be used in close
         proximity to sources of gaseous or particle emissions,
         such as smoke stacks, studies  should be conducted on
         the relationship between drift and capture and trans-
         port of atmospheric pollutants.  These studies should
         be field monitoring as well as laboratory simulation.

     B.  The need and means of controlling the emission of
         pathogens and toxic substances should be investigated,
         irrespective of any findings under epidemiology.   The
         epidemiological, microbiological, and chemical studies
         on drift may be inconclusive,  and will certainly be
         of long duration.   If public policy is to avoid poten-
         tial risks,  as it  does in public water supply,  then
         safety precautions should be imposed on cooling device
         design and operation.  Such precautions should represent
         a Best Available Control Technology (BACT).

     C.  The viability of pathogens in  drift should be studied
         to develop biological half-life projections as a function
         of atmospheric conditions.   This is necessary because
         it does not  appear that the historical concepts of aero-
         biology and  attenuation of organisms apply to dense
         aerosols.

Ill.   Modeling
                               12

-------
     Procedures should be further refined for mathematical
modeling of the health impact of cooling device drift.  This
requires more precise input on variables which have been only
assumed in this study, and better integration of drift and
infectivity models.
                               13

-------
                           SECTION 4

                          OBJECTIVES
     The objective of this study was to complete a comprehensive
review and analysis of potential hazards to humans, plants and
animals that might be caused by pathogens and toxins transported
via cooling device drift.

     For the purpose of program organization and control, the
project was divided into six tasks, which are shown on the fol-
lowing Information Flow Diagram (Figure 1) and detailed in the
following sections.  Two pathways were postulated.   The "normal
pathway" represents situations which might occur in the everyday
ambient environment under normal conditions. The worst case path-
way represents the highest possible concentrations  of pathogens or
toxic substances in cooling water, failure of water treatment
or biocide systems in the cooling device, atmospheric conditions
insuring pathogen viability, toxin integrity, and entry into a
susceptible host in sufficient concentration to produce disease.

TASK I

     The primary function within this task was to inventory the
types of pathogens and toxic substances which may be present in
cooling device drift.  The inventoried pathogens and toxins ori-
ginate in recycled industrial, municipal and/or agricultural
wastewaters,  and polluted river waters, which would be used as
cooling tower makeup.  It was deemed necessary to include pol-
luted river waters because power plants using treated effluent
will generally require a back-up source of surface  water in case
of wastewater treatment failure or supply inadequacy.

     The process of preparing the inventory was to:

     1.   List most commonly known pathogens and toxic substances
         found in wastewater and polluted water, with typical
         concentrations when known.  The microorganisms which
         were surveyed included the following groups :

              Viruses            Pleuropneumonia-like
              Richettsia             Organisms (PPLO)
              Bacteria           Protozoa
              Fungi              Invertebrate Parasites
                             14

-------
               FIGURE 1
       INFORMATION FLOW DIAGRAM

Effect of Pathogenic and Toxic Materials
  Transported via Cooling Device Drift
y
\ ' 	 •
/
^
f
\
>H
&-
w
EH
<
ft
a
s
CJ
§^
\
f
TASK 1-1
Identify pathogens and toxic substances present
in make up water.
>

TASK 1-2
Screen list for survival in cooling device
\
TASK
r
1-3
Screen list for passage into aerosol state.
\
r
TASK 2
Fate of organisms and substances as drift parti-
cles are tansported in atmosphere.
\
f
TASK 3
Direct effect of pathogen or toxic substance on:
-Animals (including humans)
-Plants
>
f
TASK 4
Potential for transmitting adverse effects from
infected organisms to animals and humans.
^
r
TASK 6
Model normal and worst case situations to develop
probability of occurrence.
N
TAS
	 X
Recommendation for fut

K 5
ure research.
•j
N
^
w
>H

-------
    Only those organisms which could reasonably be found in
    America were catalogued.  This list also included cer-
    tain foreign species which are being imported by world
    travelers, and which are recognized as potential health
    hazards.

         Chemical substances which were surveyed included:

         1.  Metals
             a.  Heavy metals
             b.  Transitional metals
         2.  Macronutrients
         3.  Micronutrients (Organic and Inorganic)
         4.  Chlorinated Hydrocarbons
         5.  Chlorinated and Ozonated Amines
         6.  Petrochemicals
         7.  Other toxic organics (e.g. Pharmaceuticals)
         8.  Industrial Chemicals
             a.  Plasticizers and other organics
             b.  Process wastes
             c.  Radioactive wastes.

2.  The list was screened and categorized as to the sus-
    ceptibility of organisms and substances to control by
    wastewater treatment or natural water purification pro-
    cesses .

3.  The list from Step 2 was further screened to character-
    ize only those organisms and substances which could be
    aerosolized.  This step required input and coordination
    with the aerosol physics aspects of the program.

         The end product of this task is a CATALOGUE which
    lists separately for each organism and toxic substance
    the following data:

         Organism name or chemical substance
         Disease name
         Medical significance
         Location of occurrence
         Frequency of occurrence
         Survivability in surface water
         Survivability in treated effluent
         Survivability in air and/or aerosol fomites
         Control methods in water or effluents

    The catalogue treats microorganisms as both infectious
    agents and allergens.  Chemical irritants were con-
    sidered in a slightly different manner including con-
    centration as a parameter, when available.

         There is also an estimated probability of occur-


                           16

-------
         rence in water.   Except for a few cases where data was
         available, this was a qualitative judgment.
TASK II
     Under this task the transport of pathogens and toxic sub-
stances in aerosol drift is assessed.  Utilizing the inventory
of substances and organisms produced under Task I,  Task II in-
vestigates three areas:

     1.  A review and evaluation of the production of drift by
         cooling devices and the atmospheric physics of drift
         particles.

     2.  The transport of toxic substance in drift.

     3.  The aerobiology of organisms in drift.

Drift Physics

     The primary objective here is to define drift as a function
of selected cooling device designs.  The data provided includes
the following:

     Drift size distribution
     Drift mass distribution
     Drift composition
     Drift emission rate based on liquid flow to air flow ratio
     Parameters as functions of wet-bulb/day, bulb temperature,
        relative humidity
     He^t capacity
     Exit velocity.

     The matrix on the following page depicts the types of
devices that were considered (Table 1).

     The analysis concentrated on the cooling devices of larger
sizes, from 109 to  1010 BTU/hr.,  since these have the greatest
impact.  Consideration was given to units which are capable of
producing drift and of using polluted water.  Although spray
pond cooling devices do produce drift they were not considered
because their impact is extremely localized.  Therefore, the
emphasis of this task is the examination of large capacity
evaporative cooling devices.

     Special attention was given to physical size.   There is a
significant difference between the mechanical type towers and
the natural draft towers in the following aspects.

     1.  The bulk of the towers is conducive to wake entrain-
         ment at elevated wind-speeds.


                               17

-------
                             TABLE 1

                    TYPES OF COOLING TOWERS
                Mechanical Draft
               Forced     Induced      Natural Draft       Mixed
Wet
Crossflow         no        yes             yes             yes
Counterflow      yes        yes             yes             yes


Wet-Dry

Parallel flow     no        yes              no              no
                         (note a)
Note a:  This type is to be considered only when in the wet mode
                               18

-------
     2.  The height of the towers contributes to the drift
         particle growth and dispersion patterns.

     3.  The height of the towers and their emissions will
         determine the potential for scrubbing action of
         chemicals and microorganisms from the local ambient
         atmosphere.

     Drift emission rate was considered.  The amount of drift is
a tower design function.  Previous design practice has been to
use as an upper limit, a guaranteed drift rate not to exceed 0.2%
of the water circulation rate.   Recent designs of drift elimina-
tors have resulted in drift guarantees of from 0.05% to 0.002%
of the circulating water flow.   These values may be interpreted
in terms of parts per million by using a design ratio, L/G, which
is the ratio of the water rate to the air rate, both in pounds
per unit of time.

     Data was evaluated on the initial drop size distribution.
This is an area of great uncertainty.  The drop size distribu-
tion in the cooling tower drift was related to tower design para-
meters.  Estimates were made of the limiting size, which must be
such that the gravitational fall velocity of a droplet is less
than the air speed at the exit of the tower.  Estimates were
also made of the water mass distribution, and finally, the
initial composition of the drift particles was described in rela-
tion to the make up water.

Transport of Toxic Substances

     The transport of toxic substances in the changing structure
and composition of the plume, relative to the ambient air, and
the distribution of drift over the terrain was evaluated.

     The following device parameters were taken into account with
respect to the incorporation of toxic substance into drift:

     Downwash
     Supersaturation
     Effect of effluent latent heat on plume rise
     Effect of saturated ambient air on plume rise
     Prediction of condensation

     Existing models were critically screened and typical re-
sults were evaluated with respect to the general task objective.
Evaluations for transport and fate were made on the basis of the
following functions:

     Selected cooling device types - considering the range of
       operational characteristics.
     Ambient seasonal climatology.
     Terrain characteristics (shoreline, valley, plains, urban,
        rural, etc.).
                               19

-------
     Conditions conducive to survival of organisms (humidity,
       UV screening, temperature).

     Deposition rates were evaluated in order to determine the
loss of compounds from the plume and concentrations of compounds
in the receiving environment.

     The effects of oxidation or photochemistry upon toxic
materials were assessed.  Taken into consideration were atmos-
pheric conditions, plume density, and particles resident time
in the atmosphere.  This evaluation essentially relates toxic
substance concentration to time and distance.

Aerobiology

     The ability of each pathogen group to be effectively
transmitted by aerosols was reviewed and documented.  This
process took into account the following factors for the
different particle size ranges:

     1.  Attenuation due to desiccation
     2.  Attenuation due to solar radiation
     3.  Protective mechanisms due to dissolved chemicals in the
           aerosols.

     This information was gathered from published scientific and
medical literature, and from personal liaison with former parti-
cipants in biological warfare (BW) study programs which have now
become declassified.
TASK III

     The potential effects upon inhalation by,  or contact with,
animals or plants.

     The arrival of a pathogen or toxic substance at a plant or
animal does not, per se,  mean the manifestation of disease.
The offender must interact with the body and overcome the body's
defense mechanisms.

     For each pathogen or toxic substance which was identified
in Task I, and which survived aerosol transport,  an assessment
was made of the probability of initiation or aggravation of
disease.  This assessment included:

     a.  A description of the normal means of entry of the
           offending agent into the body.
     b.  A description of the normal body susceptibility.

     This information was abstracted from epidemiological
literature for plants, animals and humans.

                              20

-------
     The result of this task is an estimate of the probability
of a pathogen or toxin producing disease,  after arriving within
capture range of the host.   This probability is expressed in
general terms based on an analysis of factors including:

     a.  Induced or natural immunity
     b.  Strain resistance
     c.  Synergistic or antagonistic factors
     d.  Age
     e.  Sex
     f.  Route of entry

     These factors were evaluated in relation to occurrence and
transmission, and faction.   Where possible chronic and acute
severity is also discussed for both individuals and population
groups.


TASK IV

     Potential for transporting adverse effects from affected
plants and animals to other animals and humans.

     This task is very closely related to the objectives of
Task III.  For pathogens, literature review and assessment covered
zoonoses.  Within this epidemiological evaluation, transmission
of pathogens from plants to humans considered their role as
fomites and vectors.

     An effort was made to identify those toxic substances which
would be assimilated in edible plant parts and phytoplankton.
Consideration was given to detoxification mechanisms in plants
and where possible estimates were given for residual concentra-
tions which could be consumed by herbivores.

     The data from this task was, as in Task III, incorporated
into the catalogue format for a comprehensive review of each
pathogen and toxin.


TASK V

     Conclusions and recommendations for future research.

     Regardless of the specific conclusions which were drawn from
the study, it was obvious that there is little data available.
Data gaps exist, identifying areas to be researched.  Further
comments were made in the areas of:

     1.  Theoretical and analogue simulation, and modeling.
     2.  Field measurements on the actual occurrence of pathogens
           in the drift in the vicinity of cooling devices.
     3.  Technological methods of control.
     4.  Epidemiology in the vicinity of polluted water cooling
           towers.
                               21

-------
TASK VI

     Predicative model development.

     One very useful way to evaluate the possible impact of
cooling tower drift on public health is by the establishment of
suitable predicative mathematical models.   It is clear for this
case, as in many other systems modeled, that all the desired
parameters, constants and variables may not be clearly identifi-
able or definable.  However, this does not at all preclude the
development of utilitarian models that can be modified as more
data become available and as it becomes apparent that some
"tuning" of the model is necessary as a result of experience.

     The question approached in the predicative model development
was the liklihood of a pathogenic organism or toxic substance
reaching and affecting the public.  Because answers to this
type of inquiry are probabilistic, they should be answered by
the development of a stochastic (probabilistic) rather than de-
terministic model.  Less work has been done with stochastic models
because they are more difficult to deal with.  Even so, their use
has become increasingly common as the shortcomings of completely
deterministic models became more apparent.

     There is a logical sequence involved in evaluating the
possible erosion of public health as a result of cooling tower
drift.  Some of the major events in this sequence, which were
discussed are:

     1.  Probability of occurrence of pathogens or toxic
           substances in makeup or other input waters.
     2.  Probability of survival of pathogens or toxic sub-
           stances in cooling towers.
     3.  Probability of hazardous materials being carried
           into the atmosphere.
     4.  Probability and time duration of  survival of
           hazardous materials in the atmosphere
     5.  Probability of interception by an appropriate host
           or vector.
     6.  Probability of development of harmful effects.

     Each of these events were developed from other events which
are probabilistic in and of themselves (e.g. presence of sunlight,
air and water temperatures, residence times, wind direction and
velocity, etc.).  Knowing something about  the parameters that
affect each event postulated, the predicative model was developed
for each event that establishes the possiblity of that event occurr-
ing.  A serial model, as outlined above has a condition that the
possibility of the preceeding event occurring must exist.   In the
modeling of this system as outlined, it is apparent that there
are many similarities to the extensive simulations for reliability
and availability predictions for electronic systems.  There is

                               22

-------
extensive literature on such simulations and predicative models
and as applicable serves as a base for the establishment of the
proposed predicative model.

     The problem of random events was solved by substituting for
the actual event or function, a simpler one where the desired
probability laws are obtained by drawing random numbers.  These
methods, based on game theory are called Monte Carlo methods.
These techniques have been well developed for the investigation
of predicative stochastic models such as are suited to this
study and form the basis for aspects of the model development.

     In this task, a predicative mathematical model is developed.
To the extent possible that known (or suspected) variables can  be
included, either on the basis of known or hypothetical grounds,
the model incorporates them.  Areas of question are identified
and provisions are made for incorporation of new or speculative
items as required.  Model testing is accomplished using routine
establishment of probabilities using available statistical data,
and by using Monte Carlo methods for prediction of probabilities.
The models developed are carefully documented in flow chart
design and development of algorithms.  Also the programming was
written in one of the higher level languages (FORTRAN).   This
allows for future building on the developed model as more data
becomes available from future work.
                               23

-------
                            SECTION 5
                           METHODOLOGY
     This study attempts to further define and assess the poten-
tial health hazards resulting from cooling tower drift.   Although
it has already been shown that data is lacking,  this study attempts
to answer questions and make a valid assessment  utilizing existing
sources and references.  It is certainly hoped that this investi-
gation will provide some answers,  but it will  also be considered
a significant effort to direct the need for future study.

     To complete the study a staff of outstanding subcontractors
and consultants were assembled.  The specialists and their fields
are as follows:

     1.  Aerosol Physics and Cooling Tower Emissions.
         (Subcontract to York Research Corp.,  Stamford,  Conn.)
         Edward J.  Kaplin, M.S.:   Principal Scientist.
         Alan D. Goldman:  Environmental Meterologist.
         Experience in theoretical and applied design of cooling
         devices,  and monitoring  emissions.

     2.  Microbiology.
         Henry David Isenberg, Ph.D.:   Chairman, American Board
         Medical Microbiology; Editor, Journal of Clinical
         Microbiology; Chief of Microbiology,  Long Island Jewish
         Medical Center.

     3.  Epidemiology.
         Cyrus C.  Hopkins, M.D.:   Hospital Epidemiologist, Massa-
         chusetts General Hospital;  Assistant  Physician, Massa-
         chusetts General Hospital;  Assistant  Professor  Medicine,
         Harvard Medical School.

         Robert Harold Rubin, M.D.:   Infectious  Disease  Unit,
         Massachusetts General Hospital;  Assistant Professor of
         Medicine,  Harvard Medical School.

     4.  Zoology and Animal Pathology.
         Basil P.  Tangredi, D.V.M.:   Practicing  veterinarian.

         Sydney Anderson, Ph.D.:   Curator of Mammals, American
         Museum of  Natural History,  New York.


                                  24

-------
     5.
     6.
Botany and Plant Pathology.
Leonard Weinstein, Ph.D.:  Director, Environmental
Laboratory, Boyce Thompson Institute.
         Delbert C. McCune,  Ph.D.:
         Thompson Institute.
                           Plant physiologist, Boyce
Aerobiology.
Philip Cooper, M.S.:   Formerly a research scientist at the
United States Air Force Air Medical Research Laboratory.
     These specialists were assigned their scope of work under the
task structure described in the Objectives (see Figure 2).   All
research was secondary, to be taken from known and accepted sour-
ces.  Data was submitted according to a task schedule and compiled
in house.
                                25

-------
                   FIGURE 2
            TASK ASSIGNMENT DIAGRAM

    Effect of Pathogenic and Toxic Materials
       Transported via Cooling Device Drift
TASK 1-1
Identify pathogens and toxic substances present
in make up water.
H2M, Drs. Hopkins, Rubin & Isenberg


TASK 1-2
Screen list for survival in cooling device.
H2M, Dr. Isenberg

TASK
Screen list for passage
H2M, York Research

1-3
into aerosol state.
                    TASK 2
Fate of organisms and substances as drift parti-
 les are transported in atmosphere.
 	York, H2M, Dr. Isenberg,  Mr. Cooper	
                    TASK 3
Direct effect of pathogen or toxic substance on
     - Animals (including humans)
         Drs. Hopkins & Rubin (humans)
         Drs. Andersen & Tangredi (animals)
     - Plants
         Drs. McCune & Weinstein
                    TASK 4
Potential for transmitting adverse effects from
infected organisms to animals and humans.
     Animals - Drs. Andersen & Tangredi
     Humans - Drs. Hopkins & Rubin	
                    TASK 6
Model normal and worst case situations to develop
probability of occurrence.
     Dr.  Uzzo
                    TASK 5
Recommendation for future research.
     H2M	

                      26

-------
                         SECTION 6

                          RESULTS

RESULTS OF TASK I - INVENTORY

     Disease means any pathological manifestation, caused either
by micro-organisms or by nonliving substances.  There are several
major routes for the transmission of disease:

     I.  Water
     2.  Air
     3.  Vectors (e.g. insects)
     4.  Fomites (contaminated food, dust, aerosols, etc.)
     5.  Direct contact with diseased organisms

     Of these, water is probably the most serious.  Water becomes
contaminated easily, it can transport germs or dissolved sub-
stances greater distances,  and it is universally required in
large quantities by all living things.

     The principal source of contamination with human pathogens
is thvough fecal discharge, and to a lesser extent, with other
products of human metabolism (mucus, pus, etc.).  As a solvent
and flushing agent, water picks up and carries contaminated road
and field sediments, food and industrial wastes, animal feces, etc

     Contamination of surface waters must be accepted as an accom-
plished fact due to the combination of storm runoff and the lack
of adequate sewage treatment.  Natural surface and ground waters
are also subject to purification mechanisms, such as settling,
aeration solar radiation and phagocytosis.  The extent to which
contaminated waters can purify themselves is a function of the
pollution concentration, the time available for action, and the
biological properties of the organisms.  Whether or not contami-
nated waters are capable of producing disease in humans is a
function of the etiology of either of two types of pathogens
which may be present.

     1. Certain organisms,  such as many of the enteric viruses,
        are normal inhabitants of the human intestine and con-
        tinue to exist in a circulating stock so long as humans
        are present.  Illness- does not occur, because popula-
        tions acquire immunity, either naturally or artificially.

                               27

-------
    The poliomyelitis virus is an example of such an
    organism.  Immunization prevents the appearance of
    clinical symptoms of the disease,  but the threat can
    never be removed and the unprotected human will con-
    tinue to develop the disease.

         A particular danger with these indigenous organ-
    isms is that they change to produce new strains, and
    there is no assurance that the immunity against one
    strain will protect against another strain.   While
    many avenues of research are promising, there is still
    no effective drug or body substance that will confer
    broad resistance against all present and new pathogens.

2.   Other organisms are not indigenous,  and can  be intro-
    duced only from a diseased person.   For example, if no
    cholera is present in the population,  there  can be no
    source of the bacterium.  Obviously the key  to the con-
    trol of these diseases lies in maintaining a healthy
    population.

         The United States has been very effective in achiev-
    ing a marked reduction or elimination of diseases which
    were dreaded less than a century ago.   Recently, however,
    there has been an increase in the  incidence  of those
    diseases that were considered things of the  past, because
    of the proliferation of world-wide travel.   Tuberculosis,
    for example,  has risen to such an  extent that New York
    hospitals are reopening T. B. clinics that had been closed.

         In addition to the importation of foreign diseases,
    another source of pathogenic organisms is the "carrier,"
    or one who harbors the disease organisms but does not
    manifest clinical symptoms.   Such  an individual can con-
    tinue to contaminate waters and escape detection and cure.

         The inventory of diseases which can be  transmitted
    through the water route is substantial.   Here in the
    United States,  water borne diseases have been kept under
    control by meticulous attention to the purification of
    public water  supplies,  and health  standards  for private
    water supplies,  recreational waters and shellfish.
    Although there is a national effort  to purify sewage,
    the fact remains that most of the  country has no sewage
    treatment or  only primary treatment.

         Table 2  lists those pathogens which are most likely
    to be found  in polluted waters.  Also  included in this
    list are pathogens which are indigenous abroad but may
    be introduced into the U.S.  by travelers.  Pathogens that
    may not naturally occur in surface or  ground waters,
    but may be introduced into waters  from external sources,
    are also included.

                          28

-------
     Toxic materials are introduced into surface water
as a result of raw or inadequately treated wastewater,
storm water runoff, solid waste leachate from landfills,
rainfall, dredge spoiling, and a variety of other activi-
ties.  As a result of the recent national effort to reduce
point source pollution, the quantity of toxic materials
introduced into surface water is being attenuated.
However, because of the magnitude of the non-point pol-
lution control problem, it is doubtful that surface
water pollution will ever be reduced to zero in the
vicinity of human habitation.  Cooling devices that draw
water from such areas will intake chemical substances
which will eventually incorporate into the aerosol drift.
                     29

-------
                           TABLE 2


              PATHOGENS MOST LIKELY TO OCCUR IN
             COOLING TOWER MAKEUP WATER SOURCES
Absidia corymbifera            Basidiobolus haptosporus


Absidia ramosa                 Blastomyces dermatitidis

Acanthamoeba (Naeglenia)       Bordetella spp.


Actinomyces Israeli            Bordetella parapertussis

Actinomyces keratolytica       Brucella abortus


Actinomyces spp.               Brucella canis
Adenovirus and Para influenza  Brucella melitensis
  virus

                               Brucella suis
Aspergillus spp.

                               Candida albicans
Aspergillus flavus

                               Candida spp.
Aspergillus fumigatus

                               Cladosporium  spp.
Aspergillus nidulans

                               Clostridium botolinum
Aspergillus niger

                               Clostridium perfingens
Aspergillus niveus

                               Coccidioides  immitis
Aspergillus restrictus

                               Conidiobolus  coronatus
Aspergillus terreus

                               Corynebacterium spp.
Bacillus anthracis

                               Corynebacterium diphtheriae
Bacillus cereus

                               Corynebacterium ulcerans
Bacillus subtilis

                               Cryptococcus  neoformans
Bacteriodes spp.
                              30

-------
TABLE 2 cont.

Dermatophilus congolensis

Echo virus,  coxsackie A & B,
  Polio

Enterobacteriaceae

Escherichia coli

Fuscobacterium spp.

Geotricium candidium

Haemophilus aegyptius

Haemophilus influenzae

Klebsiella pneumonia

Listeria monocytogenes

Mucor pusillus

Mucor ramosissimus

Mucor spp.

Mycobacterium spp.

Mycobacterium tuberculosis

Nocardia asteroides

Nocardia brasiliensis

Noeardia caviae

Peptococcus  spp.

Peptostreptococcus spp.

Phialophora  spp.

Proteus mirabilis

Prototheca  spp.

Pseudomonas  aeruginosa

Pseudomonas  mallei

Pseudomonas  pseudomallei
Rhinocladiella spp.

Rhizopus arrhizos

Rhizopus oryzae

Salmonella spp.

Salmonella typhi

Shigella spp.

Shigella boydii

Shigella dysenteriae

Shigella flexneri

Shigella sonnei

Sporothrix schenckii

Staphylococcus agalactiae

Staphylococcus aureus

Staphylococcus spp.

Streptococcus faealis

Streptococcus pneumoniae

Streptococcus pyogenes

Streptococcus pyogenes (Group A)

Streptococus spp.

Torulopsis glabrata

Vibrio parahemolyticus

Yersinia enterocolitica

Yersinia pestis  (Pasteurella)

Yersinia pseudotuberculosis

Zygomycetes  (Phycomycetes)

Various viruses, nematodes and
  protozoans
                               31

-------
      Salt,  although  it  is  naturally occurring  substance
 in  estuarine  waters,  might be  considered a toxic material
 for terrestrial  plants.  Salt  drift has been identified
 as  a potentially serious cause of injury to sensitive
 species  of  natural foliage and crops in the vicinity of
 cooling  devices.

      The effect  of airborn toxic material on human
 health has  been  intensively studied, but the impact is
 highly controversial.   Toxic substances, including acid
 sulfates and  nitrates,  and certain metallic compounds
 may produce acute or  chronic respiratory symptoms, includ-
 ing increased airway  resistance, asthma, bronchitis,
 cardio-pulmonary disease,  increased sputum, and even
 death.

      Allergens,  although not necessarily toxic, may
 cause asthma  and hay  fever, two of man's most  annoying
 diseases.   Airborne pollutants may potentiate  or mimic
 allergens on  sensitive  individuals.  "Red tide" aero-
 sols  from dinoflagellate blooms have been documented.

      Environmental pollutants may also be the  principal
 cause of cancer  or may  serve as co-carcinogens.  This
 has been documented for the particulates such  as asbestos
 and beryllium, and for  self-inflicted gaseous  chemicals
 from  smoking  tobacco.  The extent to which industrial and
 transportation emissions are related to cancer of the lungs,
 stomach,  intestine, and of the immune system may be related
 to  environmental  pollutants.

      The origin  of most gaseous (or small particle) pol-
 lutants  is  combustion.  Since cooling tower aerosols may
 be  generated  adjacent to combustion exhausts,   or may pass
 over  industrial  emissions,  they may contribute to the
 transport of  pollutants by solubilizing gases  or trapping
 particles in  the  aerosol droplets.   This is outside the
 scope of the  present study, but it should be recognized
 that  under  those  conditions,  cooling tower drift may
 potentiate  health problems, if not be directly responsible
 for them.

      Toxic  chemicals in cooling tower water can also ori-
 ginate directly  from the biological and chemical reactions
 that  occur  in the system.   These reactions are dependent
 upon  the characteristics of the device and the make-up
water.  All cooling devices can be subject to  the follow-
 ing:

 1.  Scaling on heat exchange  surfaces.
    Scaling is a  land adherent type of deposit caused by
    the precipitation of hardness elements from the water
    in the  form of salts or oxides.
                      32

-------
Principal scales are calcium carbonate and magnesium
silicate.  A characteristic of these salts
they have inverse water solubilities with respect to
temperature.  Increased scaling can occur as the
water temperature increases in a cooling system or
as the concentration of salts increases through eva-
poration.  Factors that determine or control scaling
include such analytical values associated with water
quality as pH, calcium content, total alkalinity,
dissolved solids and temperature.

     Scaling is generally controlled by chemical
adjustment of the alkalinity and/or recycle times.
The alkalinity is controlled by the addition of
acid - usually sulfuric acid - to maintain a pH
range between 6.0 - 7.0 and/or by using surface
active phosphates and organic agents.  It is noted
that pH also affects corrosion inhibition so that
a balance is necessary.

Sludge formation on heat exchange surfaces. Sludge
formations are caused by combinations of dirt, oil,
calcium and magnesium salts, organics, and other
chemical products, particularly phosphates.  These
fouling masses can mechanically filter dust from
circulating water and serve as focal points for
difficulties in the form of reduced heat transfer
and corrosions.   Additive control agents function
through a physical phenomenon which results in an
extremely thin layer of contaminant being deposited
on surfaces in the cooling tower system while the
rest is maintained in suspension.

Corrosion of piping, pumps and heat exchanges.
Corrosion is caused primarily by the dissolved
oxygen content of the water although a high chlo-
ride content in the water will also lead to cor-
rosion.  By the very nature of wet cooling towers,
the contact of the water with air assures that the
circulating water will be continuously saturated
with dissolved oxygen and is thus the major single
source of corrosion difficulties.

     Corrosive inhibitors are generally combinations
of chromates, zinc and phosphates.  The chromates
and phosphates act as anodic inhibitors.  They form
a surface film which restrains the anodic corrosive
reaction:
                  Fe £ Fe'""  + 2e

When used alone, anodic inhibitors must be present in


                 33

-------
    a large quantity. This can be substantially reduced
    with the addition of zinc, a cathodic inhibitor.
    the cathodic reaction is:

                 02 + 2H20 + 4e ^ 4(OH) ~

    This reaction is the rate controller and it is
    limited by the rate at which oxygen can diffuse to
    the metal surface.  The cathodic reaction generates
    hydroxide ions which increase the pH at the cathodic
    area.   The zinc precipitates with the increased pH
    and forms a barrier to oxygen diffusion:

4.  Biological fouling.
    The tremendous potential for biological fouling
    through slime, algae formation and all forms of micro-
    biological growth exists in a wet tower system because
    of the high average water temperature, oxygen - satu-
    rated environment that is provided.  The seed organisms
    are always in fresh supply by the process of scrubbing
    from the tower air as well as from any make-up water.

         In so far as the cooling tower operation is con-
    cerned, biological fouling can greatly decrease heat
    transfer.   This can be attributable not only to the
    organisms themselves but also to their metabolic pro-
    ducts.   The bulk, once deposited, serves as a further
    trap for debris, chemical and otherwise.

         The principal microbiological organisms involved
    are bacteria, fungi and algae.   The bacteria are the
    most troublesome type of organism.   Aerobic slime-
    forming bacteria thrive in tower environs both in the
    bulkwater and on exposed surfaces.

         The control of biological growths in circulating
    water requires its periodic treatment with biocides.
    Chlorine,  chlorinated phenols and non-oxydizing bio-
    cides are effective as growth controls.

         Chlorine may in some instances be depleted by
    its reactions with other treatment chemicals in a
    system.  Non-oxidizing biocides may be used to sup-
    plement chlorine treatment or as a replacement.   It
    is noted,  however, that for non-oxidizing biocides
    to be effective they must be present in toxic dosages.

         The addition of copper citrate, cholophenates,
    tribictyl tin, quaternary amines, methylene bis-
    thiocyante and chlorination are only a few of the
    biological control compounds and methods used.

                     34

-------
             5.  Delignification of wood parts.
                 Delignification of wood surfaces in a cooling
                 tower proceeds by two basic mechanisms:  chemi-
                 cal attack and biological attack.

                      The chemical attack is a function of high
                 alkalinity content of the circulating water which
                 is generally a function of high alkalinity of
                 make-up water.  This condition is alleviated by
                 pH control, usually with sulfuric acid.

                      A more important delignification mechanism
                 is the attack on the wood or fill cellulose by
                 fungi.  Their control is by preservative coat-
                 ings on wood surfaces, and chlorination and/ or
                 non-oxidizing biocides.

                      It should be noted that wood preservative
                 methods have included the impregnation of the
                 wood, prior to usage, with a copper salt followed
                 by an arsenic salt to precipitate copper arsenic
                 within the wood or the impregnation of the wood
                 with creosote.

                      Without specific consideration to the type
                 of make-up water to be used in any given drift-
                 generating cooling device,  it is seen that chemi-
                 cal additives are in routine use for reasons
                 which include:

                 a.  The assurance of continued effective heat
                     removal.
                 b.  The reduction of metal and wood deterioration.
                 c.  The minimization of investment and utility
                     costs.
                 d.  The influencing and development of design
                     practices.
                 e.  The making practical of less costly designs

     The consideration of make-up water in the form of recycled
industrial, municipal and/or agricultural wastewater must now be
evaluated in respect to treatment requirements necessary for
proper operation of an appropriate cooling device as well as for
the presence of pathogens and/or toxic chemicals that will pass
through the device.  This becomes necessary based on the premise
that each drift particle droplet will be a microcosm of the water
mixture within the cooling device itself.

     In any given operational environs, the make-up water will be
a function of local and prior usage by industrial, municipal and
agricultural sources.  It will be affected by the degree of reclama
tion treatment by each user prior to their recycle efforts.  This

                               35

-------
 treatment  effort may  range  anywhere from 0-100%,  On this basis,
 specific categorization of  any given water source, in general,
 will be difficult without prior knowledge of its past and pre-
 sent history,  and knowledge of constituent residence times.   In
 these considerations  radiological w-aste discharges are also impor-
 tant.  Of  equal importance  is the water source itself, its size
 (volume),  flusl. ,rig rate and/or drainage, or percolation rate
 separate from  the constituent residence time.

     In terms  of industrial wastes and to some extent agricul-
 tural wastes,  broad categories can be defined immediately with
 some brief delineations:

     1.  Wastes Containing  Mineral Impurities.
         Examples of  wastewater containing large and/or detri-
         mental amounts of  mineral impurities are steel-pickling
         liquors, copper-bearing wastes, electroplating wastes,
         oil-field brines,  petroleum refinery wastes and mining
         wastes.

     2.  Wastes Containing  Organic Impurities.
         The most important organic waste producers are milk-pro-
         cessing plants, meat packing establishments, breweries,
         distilleries, canneries, and medical institutions (e.g.
         hospitals, nursing homes).

               It is noted that stock-yards associated with meat-
         packing are  also a source of organics and pathogenic
         organisms.

     3.  Wastes Containing  Both Organic and Mineral Impurities.
         Some  examples include the textile industry,  laundries,
         tanneries and paper mills, as well as the fertilizer
         industry.

     4.  Radioactive Wastes.
         The wastes may originate in hospitals and research labora-
         tories and in the  laundries serving them;  in water-cooled
         nuclear reactors and chemical plants that process reactor
         fuels; and from mining operations.

     The types of substances potentially present in cooling device
drift due to agricultural and industrial wastes,  internal reactions,
leachate,  runoff and other  surface waters were considered and incor-
porated into the inventory of toxic substances.   Many of the sub-
stances examined are known or suspected carcinogens.   A list of such
substances was supplied by EPA Corvallis and then expanded to include
others  of interest.   Table  3 is a listing of the toxic substances
included in the inventory.
                               36

-------
                           TABLE 3

           TOXIC SUBSTANCES POTENTIALLY PRESENT IN

                    COOLING MAKE-UP WATER
Acenaphthene

Acetone

Acreolein

AeryIonitrile

Aldrin

Antimony and compounds

Arsenic and compounds

Asbestos

Benzene

Beryllium and compounds

Biphenyl

1,  2 Bis-chloroethyoxy
   ethane (haloether)

Bromochloroben zene
   (chlorinated benzene)

Cadmium and compounds

Carbon tetrachloride

Chlordane

Chlorinated benzenes

Chlorinated ethanes

Chlorinated napthalene
Chlorine

Chloroform

2-Chlorophenol

Chromium and compounds

Copper and compounds

Cyanides (barium, calcium, hydro-
   gen, potassium, sodium, zinc)

DDT and metabolites

Diabyl ethers

Dichlorobenzenes

Dichlorobenzidine

Dichloroethylene

2, 4 Dichlorophenol

Dichloropropane and Dichloro-
   propene

Dieldrin

2, 4 Dimethylphenol

2, 6 Dinitrotoluene

Diphenylhydrazine

Endosulfan and metabolites

Endrin and metabolites

37

-------
Ethylbenze                     Thallium and compound

Haloether                      Toluene

Halomethane                    Toxaphene

Heptachlor and metabolites     Vinyl chloride

Hexachloro 1,3 Butadiene       Zinc and compounds

Isophorone

Lead and inorganic compounds

Lindane

Mercury and compounds

Methyl ethyl ketone (Butanone)

Naphthalene

Nickel and compounds

Nitrites

Nitrobenzene

Nitrophenols (m,o,p)

Nitrosamines

Pentachlorophenol

Phenols

Polychlorinated biphenyl's
   (pcb's)

Phthalate esters

Secondary amines

Selenium and compounds

Silver and  compounds

Sodium chloride

Styrene
                              38

-------
RESULTS OF TASK I - ATTENUATION OF ORGANISMS AND SUBSTANCES

     Naturally, not every toxin or pathogen found in sources
of make-up water will enter the cooling device.   As stated
earlier, the quantity of toxic materials in surface waters is
being attenuated in a effort to reduce point source pollution.
"Common" pathogens, generally bacteria, are attenuated through
the processes of chlorination, and occasionally  sedimentation,
filtering or addition of biocides.

     The initial inventory of pathogens and toxins was screened
for attenuation through water treatment.  For the purposes of
our study, there are three general classifications of treatment;
physical, chemical and biological.

     Physical treatment normally includes settling, centrifuga-
tion, filtration and UV or nuclear radiation.  Conceivably heat
or sonic energy could also be used.   Chemical treatment is by
chlorination, or other biocides (e.g.  silver, organic alogens)
and control of pH.  More specific processes such as addition
of corrosion inhibitors, or measures required by specific indus-
tries are also included.  Biological treatment encompasses any
of the methods presently employed in treatment of sludge and
sewage.

     The following matrices (Tables 4 and 5), screen the inven-
tory of pathogens and toxins indicating those which would not
be attenuated by any means of wastewater treatment or natural
purification processes.  This study is primarily concerned with
these substances and organisms.  It is expected  that due to the
inability to control these, cooling device operators should be
concerned with their possible dissemination.

     Within the tables, the attenuating treatment(s) is identi-
fied for each pathogen and toxic substance in our inventory.
Because some of these substances may only be treated by one type
of treatment process, and others by two or three, there is a
distinction made between those retained for a worst case situation

     Under the status column are the letters P,S,T, or W.  These
indicate the following:

     P - Is not attenuated by any treatment process.  This
         pathogen is of primary importance.
     S - Attenuated by one process,  therefore, the organism or
         substance is of secondary importance.  It would be a
         significant concern should the appropriate treatment

                                39

-------
    process fail for any of these.
T - These pathogens or toxins can be controlled by two
    processes.  It is less likely that both treatments
    should fail, or not be applied.   Therefore, these
    are of tertiary concern.

W - It is least likely that pathogens and toxins which
    may be treated by all three types of treatment pro-
    cesses will be present in make-up water.   These are
    reserved for a worst case situation.
                         40

-------
                             TABLE 4

                    ATTENUATION OF PATHOGENS

                   AND STATUS TN MAKE-UP WATER
PATHOGEN
Absidia corymbifera
Absidia ramosa
Acanthamoeba (Naegleria
Actinomyces Israeli
Actinomyces keratolytic
Actinomyces spp .
Adenovirus and
Parainfluenza
Aspergillus spp.
Aspergillus flavus
Aspergillus fumigatus
Aspergillus nidulans
Aspergillus niger
Aspergillus niveus
Aspergillus restrictus
Aspergillus terreus
Bacillus anthracis
Bacillus cereus
Bacillus subtil is
Bacteroides spp.
Basidiobolus haptosporu
Blastomyces dermatitidi
Bordettela spp.
Bordettela parapertussi
Brucella abortus
Brucella canis
Brucella melitensis
Brucella suis
Candida albicans

TREAT
Biological
X
X
)
a. x
X
X
X
X
X
X
X
X
X
X
3 X
3 X
X
3 X

MENT PROCE
ChemJ cal
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
x
X
X
X
SS
Physical
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
STATUS
W
w
T
W
T
p
W
W
W
w
w
w
w
w
s
w
T
W
w
w
w
w
s
s
s
s
T
P - of primary concern
S - of secondary concern
T - of tertiary concern
W - retain for worst case
                              41

-------
                             TABLE 4

                    ATTENUATION OF PATHOGENS

                   AND STATUS IN MAKE-UP WATER
PATHOGEN
Candida spp«
Cladosporium spp.
Clostridium botulinura
Clostridium perfingens
Clostridium tetani
Coccidioides immitis
Conidiobolus coronatus
Corynebacterium dipthei
Corynebacterium spp .
Corynebacterium ulcerar
Cryptococcus neoformans
Dermatophilus congolens
Echovirus, Coxsackie
A & B, Polio
Enterobacteriaceae
Escherichia coli
Fuscobacterium spp.
Geotricium candidium
Haemophilus aegyptius
Haemophilus influenzae
Histoplasma capsulatum
Klebsiella pneumonia
Listeria monocytogenes
Mucor pusillus
Mucor ramosissimus
Mucor spp .
Mycobacterium spp.
Mycobacterium tuberculc
Nocardia asteroides

TREAT
Biological
X
iae
s

IS X
X
X
X
X
X
X
X
sis x

MENT PROCE
Chemical
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
ss
Physical
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
STATUS
T
T
S
W
S
T
T
T
T
T
T
W
P
W
W
W
W
W
W
T
W
T
T
T
T
W
T
P - of primary concern
S - of secondary concern
T - of tertiary concern
W - retain for worst case
                              42

-------
                             TABLE 4

                    ATTENUATION OF PATHOGENS

                   AND STATUS IN MAKE-UP WATER
PATHOGEN
Nocardia basiliensis
Nocardia caviae
Peptococcus spp .
Peptostreptococcus spp.
Phialophora spp.
Proteus mirabilis
Prototheca spp.
Pseudomonas aeruginosa
Pseudomonas mallei
Pseudomonas pseudomalle:
Rhinocladiella spp.
Rhizopus arrhizus
Rhizopus oryzae
Salmonella spp.
Salmonella typhi
Shigella boydii
Shigelld dysenteriae
Shigella flexneri
Shigella sonnei
Shigella spp.
Sporothrix schenckii
Staphylococcus agalacti:
Staphylococcus aureus
Staphylococcus spp»
Streptococcus faealis
Streptococcus pneumonia*
Streptococcus pyogenes
Streptococcus pyogenes
(Group A)
TREAT
Biological
X
X
X
X
X
X
X
X
X
X
X
X
X
X
L6 X
X
X
X
k
X
MENT PROCE
Chemical
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
ss
Physical
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
STATUS
T
T
W
W
T
W
W
W
T
W
T
T
T
W
W
W
W
W
W
W
W
W
W
W
W
T
T
W
P - of primary concern
S - of secondary concern
T - of tertiary concern
W - retain for worst case
                             43

-------
                             TABLE 4

                     ATTENUATION OF PATHOGENS

                    AND  STATUS  IN MAKE-UP WATER
PATHOGEN
Streptococcus spp .
Torulopsis glabrata
Vibrio parahemolyticus
Yersinia enterocolitica
Yersinia pestis
(Pasteurella)
Yersinia pseudotuber-
culosis
Zygomycetes (Phycomycett
Var. Nematodes,
Protozoans, and virus*
TREAT
Biological
X
X
X
X
X
X
•s) x
!S
'MENT PROCE
Chemical
X
X
X
X
X
X
X
SS
Physical
X
X
X
X
X
X
X
STATUS
W
W
W
W
W
W
W
p
P - of primary concern
S - of secondary concern
T - of tertiary concern
W - retain for worst case
                             44

-------
                             TABLE 5

                ATTENUATION OF TOXIC SUBSTANCES

                   AND STATUS IN MAKE-UP WATER
TOXIC SUBSTANCE
Acenaphthene
Acetone
Acrolein
Acrylonitrile
Aldrin
Antimony and compounds
Arsenic and compounds
Asbestos
Benzene
Benzidine
Beryllium and compounds
Biphenyl
Cadmium and compounds
Carbon Tetrachloride
Chlordane
Chlorinated Benzene
Chlorinated Ethanes
Chlorinated Ethylenes
Chlorinated Napthalene
Chlorine
Chloroform
2-Chlorophenol
Chromium and compounds
Copper and compounds
Cyanides
DDT and metabolites
Diabyl Ethers
Dichlorobenezenes
i
TREAT
Biological

X






X



















,
MENT PROCE
Chemical





X
X



X
X
X









X
X
X




SS
Physical
X
X
X
X
X
x


X
X

X

X
X
X
X
X
X
X
X
X



X
X
X

STATUS
S
T
S
S
S
T
S
S
T
S
S
T
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S
S

P - of primary concern
S - of secondary concern
T - of tertiary concern
W - retain for worst case
                              45

-------
                             TABLE 5

                ATTENUATION OF TOXIC SUBSTANCES

                   AND STATUS IN MAKE-UP WATER
TOXIC SUBSTANCE
Dichlorobenzidine
Dichloroethylene
2,4 Dichlorophenol
Dichloropropane and
Dichloropropene
Dieldrin
2,4 Dimethyl Phenol
2,6 Dinitrotoluene
Diphenylhydraz ine
Endosulfan and metabol
Endrin and metabolites
Ethylbenzene
Halo Ether
Halo Methane
Heptachlor and meta-
bolites
Hexachloro-1 , 3-Butadie
Isophorone
Lead & inorganic com-
pounds
Lindane
Mercury and compounds
Methyl Ethyl Ketone
(Butanone)
Naphthalene
Nickel and compounds
Nitrites
Nitrobenzene
TREAT
Biological






X
X

.tes

X




*ie






X
X


X
'MENT PROCE
Chemical



















X

X



X


SS
Physical
X
X
X
X

X
X
X
X
X
X
X
X
X

X
X
X


X


X
X

X
X
STATUS
S
S
S
S

S
T
T
S
S
S
T
S
S

S
S
S

S
S
S

T
T
S
S
T
P - of primary concern
S - of secondary concern
T - of tertiary concern
W - retain for worst case
                              46

-------
                             TABLE 5

                ATTENUATION OF TOXIC SUBSTANCES

                   AND STATUS IN MAKE-UP WATER
TOXIC SUBSTANCE
Nitrophenols (ra,o,p)
Nitrosamines
Pentachlorophenol
Phenols
Phthalate Esters
Polychlorinated
Biphenyl's (PCB's)
Secondary Amines
Selenium and compounds
Silver and compounds
Sodium Chloride
Styrene
Thallium and compounds
Toluene
Toxaphene
Vinyl Chloride
Zinc and compounds

TREAT
Biological




X








X




MENT PROCE
Chemical




X



X
X


X



X

SS
Physical
X
X
X
X
X

X
X
X

X
X
-X
X
X
X


STATUS
S
S
S
S
W

S
S

S
S
S
T
T
S
S
S

P - of primary concern
S - of secondary concern
T - of tertiary concern
W - retain for worst case
                               47

-------
      After screening the lists  of  pathogens  and  toxins  and  their
 methods of attenuation,  a "FAILURE"  list was derived.   The  patho-
 gens included in this list,  Table  6,  are retained  to be used  in
 generating "WORST CASE"  probabilities, should all  methods of
 attenuation fail.

      The same screening  was  applied  to the list  of toxins.  It
•was  determined that  none may be retained in  a "FAILURE" list.
 The  toxic substances which are being  examined in this study may
 only be attenuated by one or two of  the possible three  types  of
 treatment.   Because  they have fewer means of control, toxic sub-
 stances are more subject to  treatment system failure.
                            TABLE 6

  PATHOGENS  POTENTIALLY  PRESENT ONLY IN A WORST CASE SITUATION
Absidia  corymbifera
Absidia  ramosa
Actinomyces keratolytica
Aspergillus flavus

Aspergillus fumigatus
Aspergillus nidulans
Aspergillus niger
Aspergillus restrictus

Aspergillus terreus
Bacillus cereus
Bacteroides spp.
Basidiobolus haptosporus

Blastomyces dermati+idis
Brodetella spp.
Bordetella parapertussis
Clostridium perfingens

Dermatophilus congolensis
Enterobacteriaceae
Escherichia coli
Fuscobacterium

Geotrichium candidium
Haemophilus aegyptius
Haemophilus influenza
Listeria monocytogenes

Mycobacterium tuberculosis
Peptococcus spp.
Peptostriptococcus spp.
Proteus mirabilis
Prototheca spp.
Pseudomonas aeruginosa
Pseudomonas pseudomallei
Salmonella spp.

Salmonella typhi
Shigella boydii
Shigella dysenteriae
Shigella flexnei

Shigella sonnei
Shigella spp.
Sporothrix schenckii
StaphylococGus agalactiae

Staphylococcus aureus
Staphylococcus spp,
Streptococcus faealis
Streptococcus pyogenes (Group A)

Streptococcus spp.
Torulopsis glabrata
Vibrio parahemolyticus
Yersinia enterocolitica

Yersinia pestis (Pasteurella)
Yersinia pseudotuberculosis
Zygomycetes (Phycornycetes)
                               48

-------
Aerosolization of Organisms and Substances.

     The next step was to screen the remaining pathogens and all
of the toxic substances to determine which could become aerosol-
ized.  The results are shown in Tables 7 and 8.

     Essentially the pathogens and toxins which are listed in
these tables are those with which cooling device operators should
be most concerned.  These may be controlled by only one or two
means of treatment, if any.  Should the appropriate means fail,
these organisms and substances may become aerosolized.   Further
evaluation in the study discusses the aerosol transport of,  and
potential health effects on plant, animal and human life from
these pathogens and toxins.

     Complete discussion of the treatment methods and aerosoliza-
tion of each pathogen and toxin is found in the Catalogue of
Aerosol Drift Health Hazard Assessment, Appendix A. (Volume II)
                           TABLE 7

     SCREENED PATHOGENS CAPABLE OF BECOMING AEROSOLIZED
Actinomyces israeli
Actinomyces spp.
Bacillus anthracis
Bacillus subtilis

Brucella abortus
Brucella canis
Brucella melitensis
Brucella suis

Candida albincans
Candida spp.
Cladosporium spp.
Clostridium botulinum

Clostridium tetani
Coccicioices immitis
Conidiobolus coronatus
Corynebacterium spp.

Corynebacterium ulcerans
Cryptococcus neoformrns
Histoplasma capsulatun
Mucor pusillus
Mucor ramossissimus
Mucor spp.
Mycobacterium spp.
Nocardis asteroides

Nocardia brasiliensis
Nocardia caviae
Phialophora spp.
Pseudomonas aeruginosa

Pseudomonas mallei
Rhinocladiella spp.
Rhizopur arrhizus
Rhizopus oryzae

Streptococcus pneumoniae
Streptococcus pyogenes
Var.  nematodes, protozoans and
  Viruses
                               49

-------
                            TABLE 8

            TOXINS CAPABLE OF BECOMING AEROSOLIZED
 Acetone
 Acrolein
 Acrylonitrile
 Arsenic and compounds

 Asbestos
 Benzene
 Cadmium and compounds
 Carbon Tetrachloride

 Chlordane
 Chlorinated Ethanes
 Chlorinated Ethylenes
 Chlorine

 Chloroform
 2-Chlorophenol
 Cyanides
 2,4- Dichlorophenol

 Dieldrin
 2,4- Dimethylphenol
Endrin and metabolites
Halomethane
Heptachlor and metabolites
Mercury and compounds

Methyl Ethyl Ketone (Butanone)
Napthalene
Nitrites
Nitrobenzenes
Nitrophenol
Phenols

Secondary Amines
Selenium and compounds
Styrene
Toluene

Vinyl Chloride
RESULTS OF TASK II - TRANSPORT

     The function of this task is to provide the necessary infor-
mation for determining the potential for the transport of patho-
genic organisms and toxic substances via atmospheric dispersion
of drift produced by cooling systems.   The function of a cooling
system is to move waste heat from a primary system to a heat
sink, usually air or water, where the heat is dissipated.  Of
the many types of cooling systems,  the ones that produce drift
utilize evaporative cooling where heat is dissipated to the at-
mosphere by evaporation of a portion of the water used for cool-
ing.  The vast majority of evaporative cooling systems utilize
cooling towers.

     In the process of circulating  water through a cooling tower,
a small percentage of the water splashing over the fill becomes
entrained in the exiting air flow.   The entrained water is in the
form of liquid droplets.  These droplets constitute the cooling
                               50

-------
tower drift and have essentially the same chemical composition
as the circulating water.  The drift droplets are dispersed into
the atmosphere and deposited downwind of the tower.   Therefore,
if there are pathogenic and/or toxic substances present in the
circulating cooling water, these same substances can be trans-
ported from the cooling tower into the atmosphere as part of a
liquid droplet.  A number of droplets reach the ground before
they have completely evaporated.  Any pathogens or toxins found
in the remaining droplets evaporate reaching the ground as dry
particles.

     In order to evaluate the potential for the transport and
survival of pathogenic and toxic substances released in drift
it is necessary to know the cooling device and power plant
design conditions and the concentrations of pathogenic micro-
organisms and toxic substances in the make-up water.

Cooling Tower Internal Conditions.
     As stated, any one common set of specified internal cooling
tower air and water parameters can be duplicated in  all types of
evaporative cooling towers.  Therefore, the air and  water condi-
tions that do exist in a cooling tower are primarily dependent
on performance criteria specified by the power plant designer,
not on the type of cooling tower.

     Typical water and air conditions in an evaporative cooling
tower used in a large power plant application are as follows:

     1.  Incoming Air Temperature.

         The incoming air temperature is dependent on geographic
         location and time of year.  Design conditions can range
         between 19° - 28°C WB (WB = wet bulb).   The design con-
         dition is the wet bulb temperature which will not be
         exceeded more than 5-10 percent of the time.

         Toxins and pathogens will survive in these  incoming
         temperatures.  The most common temperatures used to
         incubate bacteria range from 20°C - 37°C.

     2.  Cooling Tower Plume Exit Temperature and Relative
         Humidity.

         The temperature of the plume exiting the cooling tower
         depends on the incoming air temperature and the tempera-
         ture of the water entering the cooling tower.  Values
         between 28° and 38°C are common, but higher exit temper- .
         atures do occur (43°C would not be abnormal).  Under most
         conditions the plume exiting the tower is very near satu-
         ration (or 100 percent relative humidity).   Therefore,
         the temperatures given are dry bulb and wet bulb.


                                51

-------
                                              FIGURE  3
           TYPICAL  NATURAL  AND  MECHANICAL DRAFT COOLING TOWERS
                                                                      ttt
                                                                                     Air out
                                                        Water in
                                                           Packing-
                                   Drift
                                   eliminators

                                  /
                                  ,. Pocking
                                                        Water out
                                                                      I   *  M   .\
                          -Water in
                                              Water ouf-^i. Mechanical-draft tower along with natural-draft tower are two
                                             	^V^-'^Vmain types. Former uses one or more fans to move large
                                                  ,'Ii'    quantities of air through the unit: latter has no fans.'Foroed-draft ,
                                                 •'*%£?"•!;,, design above pumps air through packing, where evaporative heat|
                                                 t!§£-  '*'»- transfer takes place
 Natural-draft tower is hyperbolic in shape, acts like a huge
 chimney. Heavier outside air enters around base, displaces
 lighter air in tower, forcing it out the top
                         a
                                                                Wafer in
                                                                                                  Pact ing,
Water in
                                          -Packing
Induced-draft tower draws air upward, through packing via fan at
top. as opposed to forced-draft type where fan is at bottom (see
lower drawing opposite)
                                                         Crossflow tower moves air normal to flow of water, as opposed to
                                                         rounterflnw type above, which moves air directly counter to _
                                                         water flow. Both towers on this page are mechanical-draft with
                                                         fans at top to give induced flow of air through packing. Exit speed
                                                         is approximately 30 miles per hour
                                                     52
                                                                                    d
  SOURCE:    (ELLIOT,   1973)

-------
         Again these temperatures are within the optimal range
         of incubation temperatures.   The most common tempera-
         ture used to incubate total  coliform bacteria is 35°C;
         and 45°C for fecal coliforms.  The toxins with which
         we are concerned should remain stable.

     3.  Plume Exit Velocity.

         Mechanical draft towers emit drift at the rate of 35 ft/
         sec.  Natural draft towers give off plumes at the
         approximate rate of 5 ft/sec.  It is at these speeds
         that pathogens or toxins entrained in the drift will be
         emitted.

     4.  Cooling Tower Exit Water Temperature (cold water
         temperature).

         This temperature is in practice the same as the tempera-
         ture of the water in the cooling tower basin.  Since
         the design cooling range of  most large towers is 11°C -
         17°C, the cold water temperature is usually between
         32°C - 38°C, a favorable range for most bacteria.  This
         is not expected to affect chemical substances.


Relevant Power Plant Design Information.
     Simplified flow diagrams for typical 1000/MW fossil-fuel and
nuclear power plants are given in Figures 4 and 5.  The following
concepts are pertinent to our discussion of tower design para-
meters .

     1.  The circulating water (i.e.  the water flowing from the
         cooling tower to the condenser and back to the cooling
         tower) never comes in physical contact with the main
         steam from the turbine or the condensate return.

         Although temperatures do exist in a typical power plant
         which would thermally destroy pathogens, since the cir-
         culating water is not exposed to these conditions, any
         pathogens present, will not  be destroyed by exposure to
         extreme heat.

     2.  The time it takes for the circulating water to make one
         "round trip" (i.e., from the cooling tower basin to the
         condenser, back to and through the cooling tower) can
         vary between 2.5 minutes and approximately 2 hours.
         This is dependent on the size of the tower basin.

         If pathogens are capable of  surviving, and toxins
         remain stable in water, whether they are exposed for
         two minutes or two hours should have no effect.


                                53

-------
                                                        GENERATOR,1000 mw
                                                        [3.4 x 109 BTU/HR.)
       STACK LOSSES  0.9  x 10
              tt
   9 BTU/HR.


FOSSIL-FUEL STEAM PLANT
                                                                 AIR FLOW EVAPORATION
                                                                 AND DRIFT  8955  gpm
                                                                 (33.90 M3/min.)
Or
    FUEL

    9.0 x 109

      BTU/HR.
                          CONDENSATE RETURN
                                                                              SLOWDOWN
                                                                              2239  g
                                                                              (8.47
       IN-PLANT LOSSES
       0.4 x 109 BTU/HR.
                      CONDENSER FLOW 430,000 gpm
                                                     iju,uuu yFm        i
                                                     (33.89 M3/min.)    /
                                         MAKEUP WITHDRAWAL 11,194 gpm-^
                                                           (42.37 M3/rain.)
FOSSIL-FUEL STEAM ELECTRIC PLANT USING HYPERBOLIC  TOWER HAS A HEAT
                     BALANCE AS SHOWN FOR  1000-MW  CAPACITY
                                          FIGURE  4

-------
Ul
en
      FUEL 	?

    10.3 x 10(
                          - NUCLEAR  STEAM
                                PLANT
 GENERATOR,1000 MW
 (3.4 x 109 BTU/HR)
    IN-PLANT LOSSES
    0.5  x 109 BTU/HR.
               NUCLEAR
               STEAM
               GEN.
                        CONDENSATE  RETURN
                                                               AIR FLOW  EVAPORATION
                                                               AND DRIFT 10,000 g; .m
                                                                  (37.85  M3/min.)
CONDENSER FLOW  475,000  gpm
                (1797.88 M3/min/)
MAKEUP WITHDRAWAL  12,500  gpm
                   (47.31  M3/min.)
                                                                                    TOWER
                                    BLOWDOWN
                                    2500 gpm
                                    (9.46 P/min. )
            NUCLEAR STEAM-ELECTRIC PLANT  USING  HYPERBOLIC TOWER HAS A HEAT
            'BALANCE AS SHOWN FOR 1000-MW  CAPACITY.
                                         FIGURE 5

-------
     3.  The amount of water released as drift is approximately
         0.005 percent of the circulating water flow.  In a
         modern cooling tower the drift rate can be as low as
         0.001 percent.  The amount of water released through
         evaporation is approximately 2.0 percent of the circu-
         lating water flow.  The amount of water released as
         blowdown is approximately 0.5 percent of the circula-
         ting water flow.  The amount of make-up water (i.e.,
         water usually taken from a river, lake or ocean to
         replace the water losses given above is approximately
         2.5 percent of the circulating water flow.

         These factors may be used in calculating the water loss
         through drift emission.  If the concentrations of
         pathogens and toxins in the water are known then one
         can roughly calculate the quantity of these agents
         emitted in drift.

     4.  The time that the circulating water is actually circu-
         lating through the condenser is on the order of 10-20
         seconds.  The temperature of the saturated steam
         entering the condenser is typically about 103°C.  The
         temperature of the condensate return is approximately
         88°C - 930C.

Chemical Treatment of Circulating Water.
     A large range of treatment chemicals are available today to
meet the four major categories of cooling water problems - corro-
sion, scale and deposits, fouling, and microbiological growth.
Some of the major chemicals used in cooling tower circulating
water treatment are identified in Table 9.  These are among the
candidates for consideration in this study.

     The most commonly used chemical corrosion inhibitors are
chromates,  polyphosphates, zinc, molybdenum, ferro-cyanides,  and
organics.  Chromic acid and its salts provide the base for the
most popular and cost/effective corrosion inhibitors in use today,
The chromates,  considered anodic inhibitors, are often formulated
with other inhibitors such as zinc, molybdenum and phosphates.
The discharge of chromate, in its hexavalent state, is likely
to be severely restricted as we proceed into an era of greater
regulatory control and enforcement.  The most commonly accepted
effluent guideline limits plant chromate discharges to 0.05 mg/1
as hexavalent chromium and total chromium to 1.0 mg/1 and less
in many individual situations.

     At the present,  chlorine is the most popular oxidizing agent
used to control microbiological growth.   Chlorine is usually
batch fed;  an average application might be 0.5 mg/1 chlorine for
one-half hour every four hours.
                                56

-------
                            TABLE 9

               COMMON WATER TREATMENT CHEMICALS


Chemical             Purpose              Concentration

Chlorine (usually                         1-2 ppm free chlorine
batch fed)           microbiocide         for 2-4 hours

chromates            corrosion inhibitor  20-40 ppm


zinc                 used in conjunction  2-3 ppm
                     with chromate

Phosphates           corrosion inhibitor  4-6 ppm as total
                     (substituted for     phosphate
                     chromates)

Polymers             silt dispersion      5-10 ppm
(e.g. poly
acrylic acids)

Phosphonates         scale control        5-10 ppm
     The phosphonates represent a relatively new and extremely
useful class of scale control agents.  Several types of these
compounds may be found in general cooling water scale control
use.  Among the most popular versions is an aminomethylene-
phosphonate compound that employs the highly stable carbon to
phosphorus bond.
                              57

-------
     Some of the commonly used scale inhibitors are polyphos-
phates, phosphonates, phosphate esters, polyacrylates,  and
sulfonated polystyrenes.  In addition to being classified as
corrosion inhibitors, the polyphosphates may also function as
scale inhibitors at "threshold" levels.  It is thought  that
polyphosphate is adsorbed on the growing face of calcite crys-
tals, aborting normal growth patterns and reducing the  hard
scale normally associated with precipitating calcium carbonate.


Transport of Toxic Substances as a Function of Drift
Characterization.

     Cooling tower drift is defined as mechanically entrained
water droplets which are generated inside the cooling tower and
carried along with the air flowing through the tower and ex-
hausted to the environment.  (Chen et al.  1977)  As defined,
these water droplets have essentially the same chemical composi-
tion as the circulating water in the cooling tower.  Therefore,
toxic substances would retain the same concentrations in drift
as in the circulating water.

     Most drift loss guarantees are quoted as a percent of the
circulating water rate with a tacit implication that the drift
impurity level is the same as that of the water circulated.  We
differentiate between drift and the liquid water added  to the
air due to condensation during the cooling of the tower plume
since condensed water is "pure" water.   Evaporated water is not
objectionable from the standpoint of adding an impurity to the
environment.  However, the addition of moisture, contributing to
a change in relative humidity, may be undesireable.  In the evalu-
ation of drift and its potential environmental hazard,  we are
ultimately interested in the total quantity of drift droplets
discharged to the environment, their chemical impurities,  and
the subsequent behavior of this drift as it interacts with the
environment.

     To assess the environmental significance of drift  it is
necessary to establish the actual total drift emission  rate
from towers of the type found in industry  today.  The drift
particle size and mass distributions must  be determined before
the dynamic and thermodynamic behavior of  the drift as  it
interacts with the environment can be evaluated.

Particle Size and Mass Distribution.
     Figure 6 presents the drift droplet size and mass  distribu-
tion respectively at the stack discha.rge based on field tests.
(Wistrom and Ovard, 1973).  Note that these tests were  run on
towers where the total drift loss was measured at 0.001 percent
of the circulating water rate and therefore, are representative
of the current state of the art of drift eliminator designs.


                                58

-------
   100
                                      FIGURE 6
    10
    1.0
OC
UJ
00
    O.I
UJ
o
oe
UJ
QL
   0.01
  0.001
                             PERCENT  BY NUMBER VS

                             DROPLET  SIZE.
 0.0001
      10
too
1000
10,000
                DROPLET SIZE, MICRON

                       59

-------
               KEY T0~ FIGURE 6

SIZE AND MASS DISTRIBUTION OF DRIFT PARTICLES
 DROPLET DIAMETER
  (MICRON)
% OF SAMPLE
BY NUMBER
% MASS BY
DROPLET SIZE
22
29
44
58
65
87
108
120
132
144
174
300
450
600
750
900
1050
1200
1350
2250
2400
24.0
36.0
26.0
6.3
4.0
1.4
0.67
0.43
0.28
0.26
0.65
0.11
0.027
0.011
0.0055
0.0033
0,0024
0.0019
0.0016
0.00095
0.0010
0.43
1.49
3.76
2.09
1.86
1.56
1.43
1.26
1.09
1.32
5.81
5.04
4.17
4.01
4.00
4.03
4.57
5.46
6.80
17.99
21.83
                     60

-------
     Examination of these results reveals several important
aspects of drift.  First, it is noted that the exhaust drop size
distribution is bimodal with peaks in the 35 micrometer and 200
micrometer size ranges respectively.   In contrast, natural atmo-
spheric aerosols exhibit a unimodal size distribution.  This
difference is not surprising when one considers that the air
entrained drops in a cooling tower are both generated and
removed by mechanical means within a few seconds.  Secondly,
whether bi-or uni-modally distributed the droplet sizes are
capable of carrying two to thousands of particles or bacterium
in each droplet.

Fall Velocity of Entrained Droplets
     The terminal fall velocity of a drop is established when
the aerodynamic drag force is equal to the weight of the drop.
It has been shown that larger drops are not spherical, and in
fact experience a marked flattening on their lower surface which
materially affects fall velocity.  The fall velocity drop size
relationship is shown in Figures 7 & 8.  Droplets smaller than 100
micrometers have fall velocities which are extremely low, in-
dicating that weight of these small drops has a minor influence
on their dynamic behavior.  Thus, their path and position and
that of entrained toxins and pathogens will be primarily
governed by aerodynamic forces; most important of which are wind,
buoyancy of the exhaust plume, and vertical eddies or turbulence
in the atmosphere.  Plume buoyancy and vertical atmospheric
turbulence will tend to keep these small droplets in suspension
for an extended period.  The small droplets will essentially
follow the plume path and their concentration at any point down-
wind will be governed by atmospheric dispersion.  If the atmos-
phere is cold, the exhaust air is rapidly cooled and becomes
super-saturated.  The small drift droplets and entrained parti-
cles that remain entrained in the exhaust vapor act as conden-
sation nuclei and tend to grow in size as long as this super-
saturated condition remains.

     Figure 6 also shows that a few drops in the 1000-2400
micrometer range are present in the exhaust air.  Even a casual
field observation shows that water droplets in this size range
are emitted from a cooling tower since they are clearly visible
and easily detected.  Field observations and drift size tests
conducted directly behind the drift eliminators showed that most
of these large droplets are generated in the tower plenum area
where impinging drift and vapor condensation accumulates on
structural members.  Some of this collected moisture is eventu-
ally reentrained as larger droplets.


Drift Physics as a Function of Cooling Tower and Power Plant
Design Conditions.

     In an evaporative cooling tower, the water containing the

                                61

-------
                        FIGURE 7



            FALL VELOCITY  OF WATER  DROPS


            AS FUNCTION OF  SIZE
  IOOO
   100
o
o
.J
Ul


-I
.J
<
   10
                                   I	I	I
                                                          I	I
     10
100
IOOO
                     DROPLET SIZEt MICRON

     SOURCE:  Wistrom and Ovard,  1973


                            62

-------
                            FIGURE 8
           20 MPH  WIND
CO

                                     r
                        31.3% OF DRIFT MASS GOVERNED

                         BY ATMOSPHERIC  DISPERSION
          130    174 186 203 228   270


                  DISTANCE TRAVELED, FEET
                                                                386


••"
:s
'.--•
1 i n m n R
           SOURCE:
           .      .    .    .     .
                  5 5

                %  OF DRIFT MASS
Wistrom and Ovard,  1973

-------
waste  heat comes  in direct contact with the ambient air flowing
through the tower.  The various types of cooling towers are
classified by the method used to create the air movement through
them.  In natural draft towers, air movement is induced by a
large  chimney utilizing the density difference between the air
inside and outside the chimney.  In a mechanical draft tower
air  is moved by fans; either the induced draft type (ID) that
•pulls  air through the tower or the forced draft type (FD) that
pushes air through the tower.  Figure 3 (a-c) shows a typical
natural draft tower and an induced draft and forced draft
mechanical draft  cooling tower.

     Towers are further classified by the relative flow direc-
tions  of the air  and water in the tower.  In a crossflow tower,
the  air flows perpendicular to the falling water (see Figure 3
(a)).  In a counterflow tower the air flows vertically upward,
counter to the falling water (see Figure 3 (b, c)).

     However, the water and air conditions that exist in a cool-
ing  tower are dependent on the performance criteria specified by
the  design engineer, not on the type of tower or its air flow
direction.  Any one set of specified internal cooling tower water
and  air conditions can be duplicated in any of the types of
cooling tower described above.  The choice of cooling tower is
usually made on the basis of economic and environmental consid-
erations rather than on the basis of achieving certain design
conditions.

     The results presented here are considered typical for most
drift  eliminator designs.  However, variations in the plenum
environment and drift eliminator design will have a significant
effect on the discharge drop size distribution.  The older drift
eliminator designs are characterized by the presence of more of
the  larger drops which appreciably increase the total drift
loss and dispersion of a greater quantum of toxins and pathogens.

     Figure 9 is a drift droplet size distribution after Chen,
(1977) averaging 5 different sets of drift dots each for natural
draft  towers and mechanical draft towers.   The only major dif-
ference between them is the maximum drop capable of being sup-
ported by the plume exit velocity.


Condensation Nuclei
     Under ambient atmospheric conditions, natural cloud droplets
are  formed by the condensation of water vapor onto microscopic
particles or condensation nuclei.

     In the past,  the actual size of these nuclei had only been
determined by indirect methods.   Recently electron microscopic
measurements have been utilized.   Presently,  condensation nuclei
are classified into three size groupings;  Aitken,  Large and

                                64

-------
  iO,000
                                     FIGURE 9
                             MASS  SIZE  DISTRIBUTION
                  PERCENTAGE OF TOTAL  MASS LESS THAN STATED
                                                             MECHANICAL
                                                             DRAFT
   1,000'
03
^
O
«
u
CO
CO

s

EH



O

Q
     100..
      10.
                     4-
1-
-f-
-4-
                     10   15  20
    30
40  50   60   70
         80 85   90
95
                                                                             98%
                                  % OF TOTAL MASS


                                        65

-------
 Giant.   The  size  range  for  each  of  these classifications  is
 found  in Table  10.

     Aitken  nuclei  are  the  most  numerous type  in  aerosols.
 Their  concentration exceeds that of large nuclei  by 2.0 or 2.5
 orders.  The concentration  of  giant nuclei  is  insignificant, on
 the  order  of several nuclei per  liter of air.  The mass of indi-
 vidual nuclei varies between 10~15  and 10-H g.   Giant nuclei
 may  have a mass as  great  as 10~8g  (see Table 10).  Pathogenic
 and  toxic  particles,  should they form condensation nuclei would
 constitute aitken nuclei.

     Given an initial distribution  of particles of every  size,
 particles  whose radius  is> 10~2U< become attached  to larger parti-
 cles due to  Brownian motion.   Those particles  with radii  greater
 than 20i\ are sufficiently heavy  to  precipitate out.  Both pro-
 cesses result in  a  distribution  of  particle size  with fixed
 upper  and  lower limits.  Between these upper and  lower limits,
 the  mass is  distributed fairly evenly.


     Condensation Nuclei Composition
     The nature (chemical composition) of condensation nuclei
 is usually studied  via  the  chemical and spectral  analysis of
 samples  of raindrops and cloud droplets.  Analysis of rainwater
 shows the  following average composition:

                1 mg/1   CI-
                2 mg/1   NA+
              3-5 mg/1   CA-t-
              5-10 mg/1   S042-
              5-15 mg/1   HCC>3

 Maximum  values  in the analysis reached 10-15 times greater than
 these averages, and minimum values  10-20 times smaller.  Other
 substances were also found  in  smaller amounts.

     Impurities may be  captured  by  raindrops during their fall.
 Analysis of  water obtained  from  cloud droplets indicates the
•chemical composition  of these  nuclei.  Analyses of aerosol par-
 ticles collected  in various atmospheric layers was carried out
 by Junge et.  al.  Chlorides were present in all samples varying
 between  tenths  of,  and  several mg/1.

     The most common  nuclei were found to contain compounds of
 chlorine,  sulfur, nitrogen,  carbon,  magnesium, sodium and cal-
 cium.  Sodium chloride  is very frequently encountered in nuclei.

     Various  types  of bacteria and  viruses  may act as condensa-
 tion nuclei.  A number  of species have been determined to be
 active ice nuclei.   They have  been  observed to initiate ice in
 supercooled  water at  -1.3°C in concentrations  of  up to 10&
 nuclei active at-5°C  per cubic centimeter of culture.  Species
                                 66

-------
                           TABLE 10

            CHARACTERIZATION OF CONDENSATION NUCLEI
SIZE
CLASSIFICATION

Aitken

Large

Giant
RADIUS (cm)
        CONCENTRATION
        (cm"3)	
5X1CT7 - 2X1CT5  42500

2X10-5 _ 1X10-4    132

1X10-4 - 10X10-4     2.195
               MASS0
               (g/M3)
                           17.

                           25.

                           41.4
Giant
 distribution
1X10-4
2X10-4
3X10-4
5X10-4
2X10-4
3X10-4
5X10-4
10X10-4
2.08
0.09
0.02
0.005
23
 4.2
 5.1
 9.1
                               67

-------
that have been specifically identified are Pseudomonas syringae,
Pseudomonas fluorescens and Erwinia herbicola.  Two other
species have not been specifically identified although they
have been shown to be active nuclei.  Many other species have
been tested for their ice nucleating ability, producing negative
results.
Condensation and Drop Formation
     The essential physics of condensation of water vapor onto
acceptable nuclei includes surface tension characteristics, .
hygroscopic effects, the rate of diffusion of water vapor to
the droplet and the rate of conduction of latent heat away from
the droplet.

     The most important factor involved in the formation of
clouds is chilling of humid air, which can happen due to the
following causes:

     1.  adiabatic expansion of air on vertical ascent,
     2.  turbulent transfer,
     3.  radiation (radiative chilling).

     Cooling of air during adiabatic expansion involves a reduc-
tion in pressure.  The main factor here is the movement of air
into higher atmospheric layers.  Average daily drops in atmos-
pheric pressure (5-6 mb/day) chill each layer of air by 1-2°
each day.  Vertically ascending air, containing unsaturated
water vapor, is cooled adiabatically by I8' for each 100 m
of ascent.

     When convection is well developed the air may rise by a
height on the order of kilometers.  This would result in a very
strong cooling trend.  Chilling of the air by turbulent transfer
and mixing depends on the vertical distribution of temperature.
In a stable stratification the upper portions of the layer in
which turbulent transfer takes place will be cooled.  If this
cooling is accompanied by the transport of nearly saturated water
vapor, its condensation may lead to the formation of stratus
clouds.

     Finally,  the third cause of chilling is radiation.  This
process is manifested by the cooling of air layers containing a
large amount of water vapor together with dust particles,  con-
densation nuclei and smoke particles.   It is also evident  in
nighttime chilling of the upper cloud boundary.  Radiation
often results in the appearance, and sometimes intensification,
of the comparatively thin nighttime sub-inversion clouds.

     In nature these processes act in combination.  However, the
prime factor in cloud formation remains the vertical movement of
air.

                                68

-------
     The act of condensation begins, air is cooled and increases
the relative humidity.  Before the relative humidity reaches
100% (in terms of a plane surface of pure water), condensation
begins on larger, more active nuclei.  When the humidity ap-
proaches 100%, these have become full sized cloud droplets.
Generally, the available water vapor is used by the larger nu-
clei and the smaller, less active nuclei remain unused.   There-
fore, the number of cloud droplets  is greatly exceeded by  the
number of available nuclei.

     An average active salt nucleus is 1 n in diameter.   When
condensation occurs on such a particle, 1 second suffices for it
to grow to the size of a small cloud droplet (10 x\) .   It will
take about 500 seconds for the droplet to grow to a large cloud
droplet (100 \\) and about 10,000 seconds (or 3 hours) for it to
grow to the size of a small raindrop (1,000 i\, or 1mm).   It
would take several days for a large raindrop to form through
condensation only.  Thus it is seen that although the condensa-
tion process is capable of producing cloud droplets,  it  is far
too slow to produce raindrops of the size actually observed.
Therefore, there must be present some mechanism or combination
of mechanisms that will cause cloud particles to join and form
raindrops.  Again, large magnitudes are involved.  It will take
about one million average cloud droplets to account for  the
water contained in a large raindrop.  The mechanism for  in-
creasing the size of cloud droplets to raindrops is called
accretion.  Accretion occurs when a larger falling drop  col-
lides with other smaller droplets.  These collisions critically
depend on the position and radii of the two drops.  Not  all
droplets that collide with the large drop adhere to it,  however
this coalescence increases rapidly in effectiveness as the drop
size increases in size.  This process is more significant than
condensation in the ultimate formation of clouds.

     Through all of these processes, condensation nuclei of
pathogenic organisms and toxic substance particles, and aerosol
droplets containing these may be incorporated into larger drop-
lets and clouds.  Under these conditions these particles and
infectious agents may travel further, potentially governed by
the weather patterns as well as local wind currents.


External Conditions Relevant to Drift Behavior
     If atmospheric air is cold, the exhausted drift is rapidly
cooled and becomes supersaturated.  The small drift droplets
remain entrained and act as condensation nuclei.  As long  as the
supersaturated condition remains, they become enlarged due to
condensation.  Drift droplets affected by this phenomenon  typi-
cally represent less than 12% of the total drift mass.  Signifi-
cant condensation occurs only during brief periods when a pro-
longed supersaturated plume conditon exists.


                                69

-------
     The  relative  humidity  of  the  atmosphere, peripheral to the
 site of drift  emission, may affect aerosol drift transport.  In
 relatively  dry areas,  any substance or pathogen which  is carried
 by  aerosol  drift,  or  constitutes the condensation nucleus of a
 droplet,  will  travel  over further  distances.

     In dry areas  the moisture in  the aerosol or drift will
 evaporate rapidly.  The particle or substance remaining will
 have a lower fall  velocity  than the initial droplet.   Its trans-
 port will not  be governed by air currents rather than  by the
 drift itself.   The ultimate deposition of these particles will
 resemble  a  Gaussian distribution.

     In relatively damp areas  organisms or substances  carried
 by  the aerosol or  comprising the condensation nuclei will be
 transported shorter distances.  The droplets will accrete mois-
 ture and  due to the additional mass, the fall velocity increases
 These droplets will fall out of the drift more rapidly, deposit-
 ing the particles  in  a more immediate area.

     The  location  and type  of  cooling device itself will affect
 local relative humidity.  In dry areas, emitted drift  will
 evaporate rapidly  creating  little  if any change in the relative
 humidity.   However, in damp areas, the air is less capable of
 absorbing this additional moisture.  There may be an appreciable
 difference  in  the  local relative humidity.

     Natural draft  towers are  less likely to affect relative
 humidity  at or near ground  level than mechanical towers.
 Mechanical  towers  due to their lower height emit drift closer
 to  ground level, and  may produce an appreciable difference in
 the moisture content  of the air.

     This discussion  on relative humidity will be particularly
 important in our subsequent analysis of direct effects on plants.


 Ice-Crystal Process
     In damp areas  or when  the atmosphere is near saturation,
.there may be problems from  precipitation modification  resulting
 from aerosol drift.   One manifestation of this problem is the
 formation of ice crystals.   Pathogenic or toxic particles may
 aid in this process,  acting as nuclei.

     Observation has  shown  that cloud droplets do not  freeze
 until the temperature is far below the freezing point. Even as
 low as -30°C,  perhaps one in a thousand droplets freeze.  As
 the temperature approaches  -40°C they freeze rapidly and at
 lower temperatures, clouds  consist of crystals.

     Liquid water  that exists  at temperatures below Qoc is said
 to  be "supercooled".   Observation has shown that freezing is
 initiated by a variety of impurities such as organisms or parti-
                                70

-------
cles of chemical substances.  The cloud droplets are exception-
ally pure as compared with water on or in the ground.

     Layers of cloud that contain a mixture of water droplets
and ice crystals are unique because the saturation vapor pressure
over ice is lower than over water.   Although the difference is
small,  it is highly significant.  In a cloud that consists of
both droplets and crystals, the actual vapor pressure  will be
a compromise between the two saturation pressures.  While the
air  is not quite saturated in respect to water, it is slightly
super-saturated in respect ot ice.   This, then,  will cause water
to evaporate from the droplets and vapor to condense on the ice
particles.   We have here a process which will cause a  few cloud
elements (those that consist of ice) to grow at  the expense of
the other elements.

     As condensation is initiated on certain nuclei, freezing in
undercooled clouds (between 0 and -40°C) is initiated  by freez-
ing nuclei, (a particle that will initiate the growth  of an ice
crystal out of a liquid water under these conditions).  Minute
ice particles are excellent freezing nuclei, and a number of
other particles (natural or man-made) will also  cause  such
growth.

     Approximate estimates and observations show that  for super-
saturations of the order of 10-12% (assuming a concentration of
about 100 per cubic meter) the ice crystals may  grow within
4-5 minutes the mass of a crystal will equal that of a water
droplet with a radius of about 100-200 i\.  This  rapid  growth by
sublimation is the reason why even thin (about 1 km thick) ice-
crystal clouds with small velocities of rising air currents can
produce precipitation bands.  These sometimes reach the earth's
surface in the form of fine, light snow or rain.

     Substantially different conditions prevail  when the ice
particle occurs in the vicinity of water droplets, as  is the
case in mixed clouds.  Conditions here are very  favorable for
sublimation growth, especially if there are many more  super-
c0oled droplets than crystals in the cloud.  When relative
humidity in the cloud decreases as a result of the sublimation
of water vapor on ice particles, conditions of phase equilibrium
over the droplets are disrupted.  The latter start evaporating,
thereby adding to the supply of moisture for crystal growth.
Thus a special process of "transfer" (distillation) of water
from supercooled droplets to crystals begins to  operate.

     In a supercooled droplet cloud rapid growth by distillation
of vapor from droplets will set in as soon as ice particles with
a radius of the order of 10~6 Cm appear.  As a result  the crys-
tals will be able to grow to large sizes until all the droplets
evaporate.   The initial excess of water vapor condenses upon
the crystals and the cloud has been completely transformed into
                                71

-------
 an  ice-crystal  cloud.   Calculations show that the role of this
 process  in  the  initial  growth of ice particles occurring in a
 medium containing  supercooled droplets is very great.

      The initial stage  of  ice-crystal growth by sublimation takes
 place far more  rapidly  (10-20 times) than the condensational
 growth of water droplets.  The point at which the process of
'accretion becomes  dominant in the further growth of the ice
 crystals (r=50-60iO  is  reached within a few minutes.

      When considering the  accretion of ice crystals it should be
 borne in mind that falling crystals have a greater capture sur-
 face  than droplets for  the same mass.  At the same time, their
 fall  velocity is lower  than that of spherical particles.  This
 accounts for the faster growth of non-spherical ice crystals by
 accretion and for  the diversity of their shapes.  It may roughly
 be  estimated that  ice particles grow 5-6 times more rapidly than
 droplets with the  same  mass.


 Precipitation (Snow) From Cooling Tower Plumes.
      During the winter  of 1975-1976 significant environmental
 effects  were observed from large natural-draft towers.  They
 produced plumes persisting as far as 70 km in which the super-
 cooled water droplets changed to ice crystals and produced
 light  snowfall.  Measurable accumulations of snow were observed
 on  the ground.  The  falling snow restricted visibility to less
 than  1600 m close  to the ground.  From December 1975 through
 March 1976, this conversion of liquid droplets to ice crystals
 was observed ten times  at several power plants.  One of these
 incidents is described  below.

      During a period of clear weather on 18 January 1976, from
 0755  to  1111 E.S.T., a  flight test was conducted in the vicinity
 of  a  plant, located  25  km northwest of Charleston, West Virginia.
 This  coal-fired plant has three hyperbolic cooling towers serving
 three  generators totaling 2900 Mw.  The weather was cold and
 clear  with temperatures of -12°C near the surface, decreasing to
 -20°C  at  1600 m above ground.  The plumes from the three cooling
 towers merged and  rose  to form a typical liquid droplet cloud
 between  900 and 1600 m.  The plumes mixed with the smokestack
 effluent  at 400 m.  The rise of the cooling tower plume stopped
 at  the base of  an  elevated temperature inversion, also at 1600 m.
 The change from supercooled droplets to ice crystals began at
 5 km  and  was complete 11 km downwind of the towers.  This ice
 crystal  cloud persisted aloft to a distance downwind of 43 km.
 Snow  began descending from the base of the plume when the conver-
 sion  from droplets to ice crystals started,  and it first reached
 the ground at 13 km.  Snowfall on the ground also continued to
 at  least  43 km.  The maximum accumulation of snow (very light
 fluffy snow) was 2.5 cm.


                               72

-------
     The ground measurements outside of the plume shadow indi-
cated that the plume trajectory had changed from the initial con-
ditions.  The visibility in the clear air was greater than 15 km,
but it was restricted to approximately 1600 m in the snow near
the ground level, as it would be in a natural snowfall.

     Snow from cooling tower plumes reached the ground only at
considerable distances from the cooling tower (a minimum distance
of 8 km was measured in one case).

     In some of the tests, natural clouds were present and snow
or snow showers came from them.  This natural snow occurred be-
fore, during, or after the observations of snow from the cooling
tower plumes.  Snow was observed from the tower plumes, however,
when it was not falling from natural clouds.  Moreover, the con-
version of the tower plumes from liquid drops to ice crystals
sometimes induced a similar change in the natural clouds, creat-
ing an obvious "hole" in an otherwise unbroken cloud deck.

     We cannot specify precisely the conditions required for
induced snow.  Observations to date have indicated that induced
snow has been associated with low temperatures and with plumes
diffusing in relatively stable conditions.  The key parameters
are air temperatures of -12°C or less and relatively stable
diffusion conditions at plume height.  The rate of water vapor
emission from the towers must be critical also, since this is
the source of the additional water vapor.

     The artificial snowfall occurs when the atmosphere is
cloudy and snow would be expected.  In tests, natural snow often
coincided with tower-induced snow or occurred soon afterward.

     However, the observations made during the winter of 1975-
1976 are not unique.  A similar snowfall was observed at Oak
Ridge, Tennessee, in 1960.  The water vapor released from clus-
ters of mechanical draft towers at the gaseous diffusion plant
at Oak Ridge approximated that from a large power plant, and
the weather conditions were similar to those which induced snow
in the Charlestown observations.  Agee has also described the
artificial inducement of snowfall, but the incident he described
appears to have been caused by a seeding effect of particles in
supercooled fog.

     The details of the Oak Ridge case are also presented here.
The snow was intermittent and fairly light.   Downwind from the
cooling towers, snow began falling about 3 miles distant and
continued to be deposited noticeably on the ground up to 5 miles.
Some very light snow was reported as far as 10 miles from the
towers late in the morning, but by noon all activity seemed to
have ceased.  The srow that was deposited 3 to 5 miles from the
cooling towers was normal in appearance with some flakes up to
1/4-inch in size.  The snow falling farther downwind was finer

                               73

-------
in structure and, in the sun, appeared almost crystalline in
nature.  Since there were no roads perpendicular to the direc-
tion of travel of the plume estimates of the lateral distance
of snow deposit were not possible.  The valley contour, however,
suggested a possible width of approximately one mile.

      The snow had been falling during the night, or at least
prior to sunrise.  Between 0800 and 0900 EST there were no
clouds outside the affected area.  In the area, the clouds
ranged from scattered cumulus at 1,000 to 1,500 feet to low
stratus (base less than 300 feet) as it snowed.  Moisture from
the cooling towers rose to an initial height of about 1,500
feet, then, as it progressed downwind, the resulting cloud
decended, intermittently reducing visibility on the ground to
less than 500 feet.  A freezing nuclei detector operating at
the time of the snowfall showed no increase of detectable nuclei
Ground Level Drift Deposition
      The drift deposition problem is a complicated one involv-
ing several interrelated processes:  the dynamics and thermo-
dynamics of drops in a rising plume, the point of which the
drops break free from the plume, dispersal by atmospheric tur-
bulence, and possible evaporation in the ambient atmosphere.
To make deposition estimates, the source characteristics of the
tower such as tower geometry, amount of water circulated,
effluent speed, droplet emission spectra, drift rate, and salt
concentration, must be known.  Calculations must be made of the
plume rise, which in part depends on the initial momentum and
buoyancy flux and on the ambient atmospheric conditions.  Drop-
let transport, which depends on meteorological conditions such
as the atmospheric relative humidity, turbulence, temperature
and its gradient, and wind velocity, must be estimated.

      Thus, we see then an already difficult problem of modeling
plume dispersion becomes considerably more complicated when we
attempt to predict deposition of drift emitted with the plume.
In addition, at the present time there is no reliable field
data on drift deposition so that existing models cannot be
evaluated as to their predictive capabilities.  Chen and
Hanna (1977) compared the results of ten different drift com-
position models.  Typical natural cooling tower input condi-
tions are given in Table 11.  The individual results are given
in Chen and Hanna (1977).  The results of the ten models were
averaged, producing the concentration factors in Table 12.  By
multiplying the concentration factor in Table 12 by the total
mass emission rate of the substance of interest (Kg/month),
the ground level concentration of the substance is determined.
The concentration is that which would be determined by averaging
the ten different model predictions using the input data of
Table 11.
                                74

-------
                           TABLE 11

      MODEL INPUT PARAMETERS FOR CALCULATION OF TABLE 12
Plume initially saturated

                          •f -f»y\m •+• <^\Tir/~i v»  T
                                       •po
Plume temperature at exit from tower, Tno = 305°K
Ambient temperature near exit from tower, Tor. = 275°K
                                           tJ vJ

Tower height = 100m

Tower exit diameter = 60m

Efflux velocity = 4.3 m/sec

Amount of circulating water = 499,338 gpm (1890 m^/min.)

Drift rate = 2 X 10~5

Water salinity = 3.45%

Wind speed = 4.3 m/sec.

Calculate salt deposition rate for a sector of 22.5°

Frequency of wind direction which blows toward the sector = 1

Ambient relative humidity = 70% (constant with height)

Isothermal ambient atmosphere (slightly stable atmosphere)

Drop size distribution:
                         Mass mean diameter
Diameter interval        for interval (urn)     Mass fraction

    0-100

  100-200

  200-300

  300-400

  400-500

  500-600

                               75
50
150
250
350
450
550
0.05
0.3
0.4
0.15
0.075
0.025

-------
TABLE 12   TYPICAL* GROUND LEVEL DISTRIBUTION OF DRIFT PARTICLES FROM NATURAL DRAFT COOLING TOWERS
DIJ
Dl
. 2
.'6
.8
1 .
1 .
2.
4 .
10
30
60
To

vv;:w j_nd
stance
(Km)
5 - .6
- .8
-1.0
G - 1.5
5-2.0
0-4.0
0 - 10.0
.0 - 30.0
.0 - 60.0
.0-100 .0
tal


Area of
22*5° Sector
(Km2)
0
0
0
0
0
2
16
157
530
1256
1963
.058
.055
.071
.254
. 344
.356
.493
.081
.144
.638
.494

Deposited**
within Sector
(Kg/month)
1246.
810.
732.
2585.
2324 .
6933.
j.7566 .
21291.
5023.
870.
59380.


% of Total
Deposition
2
1
1
4
3
11
29
35
8
1
100
.1
.4
.2
.3
.9
.7
.6
.8
. 5
.5
«

— - . ., 	
Concentration** Concentration***
within Sector Factor
(Kg/Km2_m0nth
21483.
14727.
10310.
10177.
6756.
2943.
1065.
135.
9.5
0.7

.3718
.1549
.1784
.1761
.1169
.0509
.0184
.0023
.0016
.0001


*   Average of predicted deposition values of ten models, each using the input parameters
    of Table 1 (see ref. 1).

**  Frequency of wind direction which blows toward sector = 1

*** Multiply the total mass emission  (Kg/month) from the tower by the concentration factor to
    immediately determine the ground level concentration  (Kg/Km2) - month) within the distance
    interval.

-------
      A similar analysis was performed for the drift dispersion
from a mechanical draft cooling tower.  The input data is given
in Table 13.  The results, given in Table 14 are those predicted
by one proprietary model.  Therefore, the results are probably
not as representative as those given for natural draft towers.
A comparison of the results for natural and mechanical draft
towers is given in Figure 10.


Aerobiology

      The air as a route for the spread of infectious disease
has been well documented in microbial and epidemiological liter-
ature.  It is established, beyond any question, that droplet in-
fection can spread epidemics although the transmission range is
relatively short for respiratory diseases.

      In recent years, much of the attention on the long range
transmission of infectious organisms has been in the area of
biological warfare.  The information presented here was extracted
from unclassified or declassified biological warfare documents,
or derived from personal communications with or first hand ex-
perience of individuals involved in the subject.  Some data, not
singled out, had previously been classified or was extracted
from classified sources and is now in the open literature, hence
the open literature references.

      The aerosolization of these wastes via wave action,
or other agitation was clearly demonstrated by Claude Zobel (1946)
and earlier.  Additionally, it has been shown that the air travel
and the subsequent dissipation of an aerosol from a given line
source while approximating a function of an exponential decay
pattern has nevertheless often retained its basic cloud structure
and has in this form travelled hundreds of miles due to unique
meteorological conditions.  (U. S. Army 1966, and Report 219II)
However, it must be recognized that due to the nature of the
main sources of this data pool that some information has of
necessity remained classified.

      Data used in this section is from the following sources:

      A.  Fort Detrick, Maryland.  A former United States Army
          installation dedicated to a variety of investigations
          dealing with many aspects of microbiological survival,
          metabolism, destruction and disease prevention.


      B.  Dugway Proving Grounds. Utah.  A chemical warfare site
          still in existence,  that has in the past been used for
          testing of biological and chemical agents in a variety
          of forms.


                                77

-------
                             TABLE 13


        MODEL  INPUT PARAMETERS FOR CALCULATION OF TABLE  14
                     Cooling Tower Parameters



Cooling Tower Diameter  (M)                       1606


Cooling Tower Height (M)                         18.21


Plume Exit Velocity  (m/sec)                       9_35


Circulating Water Flow Rate            717900 gpm (2717.26  M3/min)


Drift Rate (%)                                    .008

Plume Exit Temperature, dry bulb  (°K)             297.

Salt concentration  (gm/cm3)                     .00135




Particle Diameter - Microns        % of Total Mass Drift



       0-50                             49%

      50 - 100                             20%

     100 - 200                             18%

     200 - 300                              7%


     300 - 500                              4%

     500 & Larger                           2%



                     Ambient Conditions


Wind speed (m/sec)                        4002

Temperature,  dry bulb  (°K)                266.


Specific humidity (Ibs moisture/lbs dry
air)                                  "    ,00158


Pasquill stability class                   4


Frequency of wind direction which
blows toward the sector                    1


                                78

-------
co
   TABLE 14   EXAMPLE* OF GROUND LEVEL DISTRIBUTION OF DRIFT PARTICLES FROM A MECHANICAL DRAFT
                                           COOLING TOWER
	 .... — 	 	 , 	 . 	 	 ,._ . 	 	 , . _. , „ _. 	 . 	 	 	 . — 	 	 ...,., , . .....
Downwi
Distdll
(Kir.)
. 2
.6
1.
2.
4.
6.
10
15
20
2 -
- 1
0 -
0 -
0 -
0 -
.0 -
.0 -
.0 -
nd
. 6
.0
2.0
4.0
6.0
10.0
15.0
20.0
30.0
Total
Area of
22^° Sector
(Krr. 2)



2
3
12
24
34
98
176
.0612
.1257
.5890
.3562
.9270
.566
.544
.361
.174
.7
Deposited** % of Total
within Sector Deposition
(Kg/month) (%)

938.
1450.
1


1
2
981.
306.
565.
570.
160.
2130.
1
865.
12965.
7
11
15
2
4
12
16
16
14
100
.2
.2
.3
.4
.4
.1
.7
.3
.4
.
Concentration** Concentration***
within Sector Factor
(Ka/Kn2-mcnth)
15328.
11534.
3363.
130.
144.
125.
88.
62.
19.

1.19
.88
.26
.01
.01
23
36
1 7
01
12
.0097
.00
.00
.00

68
48
15


   **
   ***
Calculated from predictions of combined trajectory - Gaussian drift dispersion model
for a round mechanical draft cooling tower.

Frequency of wind direction which blows toward sector - 1

Multiply the total mass emission  (Kg/month) from the tower by the concentration factor to
immediately determine the ground level concentration (Kg/Km -month) within the distance
interval.

-------
oa
w
g
«
O
H
O

PM
O
M

i
£
H
O
iz;
8
      o
       c
      •H
       i
      CN

       I
      m
       I
       c
n~4
                                                        r  r  r • i
                 M
                 N
                      M
                     N.
                         M
                        N
                             N
                             M
                                 M
                                    JL
                                       M
                                         M
                                         N
M _.N
                                                  N
                                     10'


                             DCV/NWINO DISTANCE (km)
                                                                  10J
          Calibration Factor vs.  Downwind Distance For Examples  of

          Natural  (N) and  Mechanical (M) Draft  Cooling Tower Drift
                                   Deposition
                              FIGURE 10
                                 80

-------
    The major mission was long range field testing in a relatively
    isolated part of the state.

C.  Illinois Institute of Technology, Chicago, Illinois.
    Its research staff has contracts and grants from a large
    number of government and private sources.  The research is
    variable and in some areas possibly classified.

D.  Army Material Command, Washington, D.C. Involved with the
    logistics inherent in any aspect of the army mission.

E.  Edgewood Arsenal, Edgewood, Maryland.  At one time the research
    center for the study of gas,  aerosol,  and radiological and
    chemical properties, and the toxicity and protection from all
    forms of chemical agents.

F.  United States Navy.  Via direct or contracted efforts the USN
    was engaged in collaborative efforts with its sister services
    to investigate various aspects of biological warfare agents,
    dissemination, detection and protection.

G.  Aberdeen Proving Grounds, Maryland. Primarily the army loca-
    tion for hardware testing.

H.  Various contracts were in force at different times with organi-
    zations as diverse as Booz Allen Inc.  an-^ the University of
    Pennsylvania.

I.  Foreign sources include the Canadian experimental station at
    Suffield, Canada and the British laboratories at Porton,
    England.

J.  Wright Patterson Air Force Base, Ohio. Site of medical, physio-
    logical and toxicological testing in conjunction with the USAF
    mission.  At one time it was involved in long range bacterio-
    logical tests from the point of view of detection and preven-
    tion of a microbiological attack.


    The analysis within this task analyzes organisms in relation
to attenuation due to dessication, solar radiation and ambient
environmental conditions, and to protective chemical mechanisms.
The organisms addressed in this task are listed in Table 15.

Results of Data Search
     Serratia marcescens had been indicated as the causative
agent in the deaths of individuals following a series of Biologi-
cal Warfare  (B.W.) tests.  This occurred in two separate inci-
dents, in 1950 near San Francisco and in 1952 near Fort McClel-
lan, Alabama.

                              SI

-------
                            TABLE 15

                 ORGANISMS REVIEWED FOR AEROSOL
                  SURVIVAL AND TRANSMISSABILITY
 1.   Rust spores

 2.   Bacillus globigii

 3.   Pasteurella pestis

 4.   Pasteurella tularensis

 5.   Coccidioides immitis

 6.   Rickettsia burnetii

 7.   Serratia marcescens

 8.   Sarcina lutea

 9.   Venezuelan equine encephalitis

10.   Vibrio cholerae

11.   Enterotoxin B

12.   Simian virus

13.   Toxic proteins

14.   Klebsiella pneumoniae

15.   Escherichia coli
                                82

-------
lowered metabolic activity, permitting damaged cells to survive
and even repair, lie dormant or even recuperate from the
stress.

     B.  globiggi has also been used in field trials in the dried
spore state, in a slurry and in liquid media. (U. S. Army, 1953),


     Survial and Destruction
     Levin (1966) has cataloged the persistence of a number of
microorganisms in a spectrum of soils and climate.  Tests of
persistence and variability of persistence, have produced a
variety of techniques designed to kill microorganisms.  These
range from extreme dry heat to the use of mustard gas on E. coli
(U. S. Army 1965).  Ultra violet light has of course been used
against some organisms while other forms of radiation have per-
mitted the ultimate recovery of the cell's capacity to produce
DNA.

     A study by Lighthart (1972) employed vegetative £3. marces-
cens,  Sarcina lutea and B. subtilis spores.  The aerosols were
challenged by varying humidities at 15°C for 6 hours in a carbon
monoxide environment that approximated a high urban concentra-
tion.   The survivability varied from lethal to protected.
For example, £L marcescens was killed off four to sevenfold at
low (1-25%) RH.  Above this at 90% RH, protections was provided.
£L lutea varied fromtprotection during the first hour to death
during the next 5 hours, topping a seventy fold increase in the
RH range of 0 to 75%.   The spores of B. subtilis proved to be
hardy in almost all environments.

     Viruses and bacteria have been inactivated by ozone (Burle-
son, 1975) but the truly effective result depends on the water
being free of almost all organic waste.  River water would be
a difficult treatment media by this method, due to the ozone
demand of the organic material.

      Viruses
     Virus  aerosol  survival  studies have lagged behind bacteria
or plant  spores due to  the inherent problems of tracing, trap-
ping and  identification.  As techniques have improved we have
learned that  some viruses persist in the airborne state in good
numbers for 6 or more hours.  Using vesicular stomatitis virus
(VSV) Watkins et. al. (1965) studied temperature and humidity
variables.  He concluded that maximum stability occurred at 20%
and 80% RH  while minimal stability was to be found  at 50% RH.
Temperature increments  from  50°, 70°, 80° and 90°F  all increased
the decay rate.

     Harper (1961)  studied vaccinia, influenza and  V.E.E. at
temperature ranges  of 7-12°C, 21-24°C and 32-34°C and concluded
survival  was  inverse to temperature.  Coxsackie A21 aerosols  at
25°C and  50 to 60%  RH experience a 50% decay rate in the first
few seconds of aerosolization.

                                85

-------
     The persistence inherent in many aerosols of virus origin,
depends greatly on the initial concentration.  A dry cloud
containing virus matter exhibits a longer survival.  The medium
in which the virus is suspended in prior to, and during aerosoli-
zation influences the subsequent behavior.  The content has been
studied but not the mechanism.  Inositol for example decreases
the sensitivity of viruses to relative humidity, U.V. light and
X-ray, thereby artificially prolonging the viability.

     Survivability and inactivation of viruses in air was ex-
amined at Wright Patterson Air Force Base, Ohio with the follow-
ing conclusions. (1974).

     Adenoviruses, enteroviruses and Newcastle virus were most
stable at room temperature and 50% relative humidity.  Para
influenza and respiratory syncitial viruses were inactivated
rapidly as relative humidity shifted.  The decrease in relative
humidity decreased the survival rate of the adenoviruses and
enteroviruses within 2 hours.  At moderate (50%) or high (90%)
relative humidity the survival rate varied from 7-24 hours.
The para influenza, respiratory syncitial and New Castle viruses
were inactivated rapidly at high and medium RH and to a lesser
degree at low RH.

     In the realm of survival, Lefler and Kott (1974) noted
the survivability of polio virus Type I in dry sand for 77 days.
Wellings extracted Coxsackie 64, Polio Type II and I, Coxsackie
Type A, (Wellings, 1975) Echo (Snow, 1955; Goetz, 1954;  Erlich
& Miller, 1968) from ground water.   The significance of these
findings lies in the appreciation of the ubiquity, and persistence
of these microbiological entities with regard to survival.

     Inactivation had been attempted by Jensen when he exposed
aerosols of Coxsackie,  influenza,  sindbis, and vaccinia to ultra
violet during passage through a tube.  The kill percentage is
expressed in Table 16.   Therefore,  one may conclude that by
avoiding U.V.,  as in a dust cloud or debris environment, to some
degree, the viability of the aforementioned viruses would be
protected.

     A number of studies were directed at the behavior of or-
ganic entities such as viral nucleic acid from Simian Virus 40
(SV40) (U.  S. Army, 1956; Akers, 1972) virus protein and RNA of
Encephalomyocarditis Virus (EMV) (DeJong et^ _al, 1974), stability
of toxic proteins (U. S. Army, 1965), enterotoxin B (U.  S.  Army,
1967),

     The results concerning the survivability and persistence
of these viruses varied greatly so that no general conclusion
can be drawn.
                               86

-------
     For example:

     SV40 persisted well at 21 C throughout a spectrum of rela-
tive humidities from 22 to 88%.  At 32°C viability was practi-
cally gone within 60 minutes.

     At relative humidities of 50% or less the EMV entity lost .
its viability, yet the RNA of the virus retained its infectivity.

     The same illogicity persists with enterotoxin B and other
proteins.  However, as long as the substance retains its aller-
genic or toxic property the debilitating effects common to the
entity can occur.

     Mycotic Sources
     Coccidioides immitus is distinctly hydrophobic when in its
arthrospore stage (Levine, 1977).  As such, it survives well
within the wide ranging termperatures,  relative humidity, dessi-
cation and even light energies of an infinite variety.  Soil
conditions that provide Na*, Ca++,  S04~, and Cl~ encourage the
survival and growth of this fungus.  Therefore, any organic
mass able to provide these ions can in turn harbor this pathogen.
Moisture enhances the mycelial growth.   Dispersion by air is
easily accomplished since the intact arthrospore is extremely
persistent in air and in soil from which it can be wafted.

     Rickettsia
     Rickettsia burnetii is linked to Q fever.  Aerosols of this
agent have infected at distances of over ten miles from the point
source (Tigertt, 1961).

     Many of the effects of variables on microbiological sur-
vival in aerosols are tabulated in Table 17.  The effects were
arrived at during B.W. tests and research.  Some of the vari-
ables examined include exposure to U.V. light, temperature and
relative humidity.
                            TABLE 16

              KILL PERCENTAGE  OF VIRUSES  EXPOSED
                   TO ULTRA VIOLET RADIATION
 100  ft.3/min.  flow                       200  ft.3/min.  flow

     99.9              coxsackie                  97.5

     99.9              influenza                  99.9

     99.9              sindbis                   96.7
                      vaccinia                  99.9

     96.8              adenovirus                91.3


                                87

-------
00
00
                                      TABLE  17

                      EVENTS INFLUENCING MICROBIOLOGICAL  SURVIVAL
(1 of 5)
ORGANISM
Adenovirus
Bacillus
globigii
Bacillus
subtilis
Coccidioides
immitis
Coxsackie A21
Coxsackie B4
Echo 1, 7, 11
TEMPERATURE RELATIVE
°C HUMIDITY
70° 50%
50-90%
40
ambient ambient
ambient ambient
10°-450 5-85%
25° 50-60%
100%
100%
100%
COMMENTS OR EFFECTS AND REFERENCES
91.3% survival in an aerosol; exposed to
U.V. light (Jensen, 1964).
Stable at 22°C (USDOA, 1974).
Decay directly proportioned to rise in
relative humidity (U.S. Army, 1974).
Increased count of organism 100% survival
(Leif and Hebert , 1977).
Carried 12 mi, remained viable in field
aerosol (U.S. Army, 1953).
Survived in all environments (Lighthart,
1972).
Arthrospore survives well in all expo-
sures (Levine, 1977).
Immediate 50% decay (Jensen, 1964).
Found in ground water. Aerosol passed
through U.V. light at rate of 200'/min.;
97.5% decay (Akers, 1972).
Found in ground water (Wellings, 1975).
Found in ground water (Wellings, 1975).

-------
00
CD
                                       TABLE 17

                       EVENTS INFLUENCING MICROBIOLOGICAL SURVIVAL
                                                           (2 of 5)
ORGANISM
Encephalomyo-
caditis
Enterovirus
TEMPERATURE RELATIVE
*C HUMIDITY
50%
50%
70° 50%
50-90%
COMMENTS OR EFFECTS AND REFERENCES
Virus loses viability (DeJong, 1974).
RNA retains inf ectibility .
Stable at 22°C (U.S. Army, 1974).
Direct relationship between increase in
relative humidity and survival.
   Escherichia coli
24-30°
40-90%     Survives well;  mustard gas affects
           growth (Dimmick,  1965 & U.S.  Army, 1965).
Influenza 7-12°
21-24°
32-34°
Klebsiella ambient
pjieumonia
Newcastle virus 70°
Survival is inverse to rise in
ture (Harper, 1961).
When aerosol was exposed to U.V
decay (Jensen, 1964).
tempera-
. ; 99%
ambient Presence is key to poor environment
(Seidler, 1975).
<50% Only 1% survival after 24 hours (Goldberg,
1977).
?-50% Survival increased as relative humidity
increased (Goldberg, 1977).
50% Stable at 22°C (U.S. Army, 1974
50-90% Rapid deactivation of virus (U.
).
S . Army ,
                                                1974).

-------
                                        TABLE 17

                        EVENTS INFLUENCING MICROBIOLOGICAL SURVIVAL
                                                             (3 of 5)
   ORGANISM

   Newcastle virus
    (Continued)	
TEMPERATURE
    °C
 RELATIVE
 HUMIDITY

<50%
COMMENTS OR EFFECTS AND REFERENCES

Increasing survival rate (U.S.  Army,
1974).
   Pasteurella Pestis
              26-39%     Good survival rate (U.S. Army, 1965)

              61-87%     1 log death rate; actual 90% loss
              	(U.S. Army, 1968).	
CD
O
   Pasteurella
    tularensis
-40-24°

24-35°


490
>75%
Survives well (U.S. Army, 1964).

Death rate increases linearly (Erlich,
Miller, 1968).

Maximum kill temperature (Erlich, Miller,
1968).
   Polio type I
              0%


              100%
            Survived for 77 days in sand (Leffler &
            KOH, 1974).

            Found viable in ground water (Wellings,
            1975).                     	
   Polio type II
              100%
            Found viable in ground water (Wellings,
            1975).                        	
   Rickettsia
    burnetii
                         Proven to cause Q-fever 10 mi. from
                         origin (Tigertt, 1961).	
   Sarcina lutea
15°
 0-75%
Exposed to CO gas; survival varied
(Lighthart, 1972).         	

-------
CQ
                                        TABLE 17

                        EVENTS INFLUENCING MICROBIOLOGICAL SURVIVAL
                                                            (4 of 5)
    ORGANISM

    Serrata
     marcescens
TEMPERATURE
    °C
                        -40-32°

                        -40-120°



                        150
    Staphylococcus
     aureus
24-30°
RELATIVE
HUMIDITY

90%

20-80%

20%



1-25%



 10%
COMMENTS OR EFFECTS AND REFERENCES
                         Protected viability (Lighthart,  1972).

                         Increase in death over 32°

                         Decrease in relative humidity increased
                         death rate (U.S.  Army, 1968 and  Dimmick,
                         1965).

                         Survived for 6 hours while exposed to
                         CO gas; then 4.7  fold death rate (Lightart,
                         1971).

                         Relative humidity not a factor in survival
                         (Goetz, 1954).
Simian virus (SV) 21°
32°
Sindbis virus
22-88% Survived well
22-88% Decayed within
96
to
.7%
U.V
survival
. light
(U.S. Army, 1965).
1 hour (Akers, 1972).
when aerosol was
(Jensen, 1964).
exposed
           Survives well (Dimmick,  1965).
    Staphylococcus
     pullorum   	
              15-80%     Survivability increases as relative
             	humidity increases (Dimmick,  1965).
    Streptococcus
     salivarius
24-30°
           Survives well (Dimmick,  1965).

-------
CD
to
                                       TABLE 17


                       EVENTS INFLUENCING MICROBIOLOGICAL SURVIVAL
(5 of 5)
ORGANISM
Toxic Proteins
Vaccinia

V.S.V.

TEMPERATURE
°C
25-32°
7-12°
21-240
32-34°

50°, 70°,
80°, 90°
7-12°
21-240
32-340
RELATIVE
HUMIDITY COMMENTS OR EFFECTS AND REFERENCES
Retains potency (U.S. Army, 1965)
Survival inverse to temperature (Harper,
1961) .
Aerosol exposed to U.V. light; 99% decay
rate (Jensen, 1968).
20-80% Increase in decay as temperature in-
creases (Watkins, et . al . ) .
Survival inverse to temperature (Harper,
1961).

-------
RESULTS OF TASK III - DIRECT EFFECTS

     The potential direct effects of pathogenic organisms and
toxic substances on plants,animals and humans were evaluated
under this task.  The study of conventional epidemiology and
pathology concerns itself with the routes by which infection is
acquired, considers the transmission of disease from man to man,
that arising from contact with environmental sources, as well as
the essential nature of the disease and especially the struc-
tural and functional changes caused by it.   T"he potential of a
given agent to cause disease in any given population, and the
existence and pattern of any ensuing epidemic rests upon a number
of variables, but particularly the following:

     1.  Portal of entry for the disease-causing agent.
     2.  Portal of exit.
     3.  Incubation period.
     4.  Gradient of infection.
     5.  Mode of spread.
     6.  Survival in nature.
     7.  Susceptibility of the population at risk.


Portal of Entry

     Since the material released from the tower may be carried in
a variety of physical forms and in particles of varying size, with
different agents being present in differing quantities,  three
potential portals of entry must be considered:

     Inhalation:  Inhalation of airborne or droplet-carried
organisms may create invasive disease at any site within the res-
piratory tract.  Large particles and droplets are restricted by
normal host defenses to deposition on the mucous membranes of the
nose, mouth, pharynx, and upper tracheo-bronchial tree.   In this
instance, high concentrations of the infecting organism are usu-
ally required to produce disease, and the mechanism is usually
direct contact.  In contrast, smaller particles, 1-5 microns in
size, will be inhaled into the distal portions of the respira-
tory tract, including the terminal bronchioles and alveoli, and
these can cause disease with very small numbers of particles,
occasionally estimated to be as few as a single particle.  This
is true "inhalation disease".  For those few organisms studied,
a balance exists between size of inhaled droplet and numbers of
organisms required to transmit disease, making specific quanti-
tative estimates of disease risk impossible, except for the few
microorganisms well-studied in defined laboratory models of in-
fection .

     Contact:  Particles of any size on exposed surfaces may
cause disease in several specific areas.  Whereas contact of even
large numbers of most organisms on normal skin or surfaces is un-
                                93

-------
likely to cause disease, certain exposed surfaces may be much
more susceptible, particularly the following:

     1.  Abnormal skin or surfaces (sites of burns, abrasions,
         or open wounds).
     2.  Mucuous membranes of respiratory tract.
     3.  Conjunctiva.

     Ingestion:  Food or water supplies contaminated by cooling-
device drift can cause disease in any population ingesting such
contaminated materials.  In considering the possible role of
cooling device drift, primary contamination of food and water
supplies, as well as secondary contamination via infection of
plants or animals lower down in the food chain must be considered,
The risk of both primary and secondary contamination is particu-
larly important for any population ingesting untreated food or
unchlorinated water.
Portal of Exit

     This is a relatively unimportant facet of this task, and
is pertinent to the discussion of secondary propagation of
disease through a community.  Portals of exit are relevant by
providing a means of infecting cooling tower makeup water in the
first place.  While organisms excreted predominantly by fecal or
urinary routes of exit may find their way most readily into
polluted river or ground water, virtually any organism in any
tissue of any susceptible host, excreted by any route, such as
respiratory tract discharges, could, under some natural circum-
stances,  be found there.  What is more important, therefore, is
the ability of the organism to survive or multiply in such set-
tings .

Incubation Period

     This period between first contact of the host with the in-
fecting organism and the development of clinical symptoms plays
a major role in determining the shape of epidemic curves, and
hence,  in the development of appropriate forms of epidemiologic
monitoring of the effects of cooling towers.   However, considera-
tion of incubation periods is not relevant to this preliminary
discussion.
Gradient of Infection

     This reflects the proportion of those infected who become
symptomatically affected by the disease.   It is important in
determining the magnitude of an epidemic  and in evaluating the
public health significance.  It is largely determined by the
susceptibility of the population and the  size of the infecting

                               94

-------
inoculum.  This will become important in determining modes of
epidemiologic monitoring, in which the search for asymptomatic
infections may become required.


Mode of Spread

     The conventional modes of transmission of epidemic disease
are:

     1.   airborne
     2.   droplet
     3.   contact
     4.   food and/or water
     5.   vector.

Direct or indirect contact with cooling-device drift will involve
all these modes of spread, with the possible exception of vector-
borne disease.  In the discussion of individual infectious agents
that follows, droplet-borne diseases are considered under the
respective portals of entry of either contact or inhalation
disease depending on the anatomic site of impact of the offending
agent and the route by which specific disease might be best
acquired.
Survival in Nature

     This concept is perhaps the most important in determining
the risk from exposure to pathogens in cooling device drift.
Survival of the microorganisms includes an,analysis of what is
present in the water sources used to cool the towers, survival of
the initial "inoculum" in the highly unnatural thermal conditions
of the tower, and in the aerosol produced.  These last two con-
siderations will be the most important factors in determining
whether or not disease will ensue from the use of water polluted
with any potential pathogen.  This was of course, discussed
within Task II.
Susceptibility of the Population at Risk

     This may be the most difficult variable to define quantita-
tively, as it will differ for each organism and for each popula-
tion group.  For humans the demographic and medical variable that
will need to be considered include the following:

     1.  Age distribution of the exposed population.
     2.  Racial distribution of the exposed population.
     3.  Sex distribution of the exposed population.
     4.  Presence of malnutrition or exposure to other factors
           that will affect host defense (e.g., malaria, produc-

                               95

-------
           ing reticuloendothelial system blockade, and thereby
           increasing susceptibility to Salmonella infection).
     5.  Prior experience or exposure to the pathogens and toxins
           involved.
     6.  Presence of immunologic deficiency states.

     Variables to be considered for animals include these:

     1.  Age distribution of the exposed population.
     2.  Sex distribution of the exposed population.
     3.  Presence of malnutrition or other factors that will
           affect host defense (e.g., weather conditions,
           breeding cycles, migration and hibernation patterns).
     4.  Prior experience or exposure to the pathogens and toxins
           involved.
     5.  Presence of immunologic deficiency states.
     6.  Relative position in the trophic levels, and feeding
           pattern (e.g., herbivore, carnivore, omnivore).

     The environmental and physical variables which predispose
vegetation to infection or intoxication are more numerous and
varied than those for humans and animals.  The factors include:

     1.  Biological variables; including age, stage of develop-
           ment, species and variety of the exposed population.
     2.  Edaphic variables; soil conditions such as moisture and
           nutrition content.
     3.  Climatic factors; e.g. wind speed, temperature, rela-
           tive humidity, light intensity and quality.
     4.  Presence of a second pollutant or toxin, which may
           modify the effect of the first.
     5.  Quantity of precipitation.
     6.  Factors of exposure including the concentration of the
           toxin, duration and frequency of exposure.  (Contin-
           uous and intermittant exposures of the same dosage
           will produce significantly different effects).

     General statements as to the relative risk of specific or-
ganisms or toxins can be found in Appendix B, Aerosol Drift Direct
Effects Assessment Catalogue.  Analysis of the actual effects on
humans, animals and vegetation is included for each specific
toxin or pathogen.

     Finally it should be recognized that the risk of cooling
device drift to a given population involves not only the direct
transmission of disease and toxicity but also indirect trans-
mission (to be discussed under Task IV), and transmission of
allergens.

     Allergens of many types can be considered, but are generally
beyond the scope of this report, although several biologic agents
can be so involved.   Some, by their ubiquity and because the

                                96

-------
unique environmental situation of the cooling tower might poten-
tiate their growth or transmission,  might be particularly impor-
tant, including:

     A.  Allergic broncho-pulmonary  aspergillosis
     B.  Thermophilic actinomycetes.

     Under these conditions, high concentrations of many other
organisms, or antigenic fragments of  organisms,  might also become
important.  Among these is transmission of toxic substances which
might alter the susceptibility of the population to other organ-
isms, and resultant long-term effects of prolonged contact with
unusual chemical or biologic materials.

     One particular organism which could not be  addressed fully
within the format of the Aerosol Drift Direct Effects catalog is
Mycobacterium tuberculosis.   The general effect  of this organism
is tuberculosis, a chronic infectious disease.  It is normally
characterized by the formation of avascular nodules of inflama-
tory tissue.

     The organism is carried and transmitted via aerosol formites
while infection may occur through inhalation, ingestion or di-
rectly through skin . Inhalation is the most frequent means of in-
fection.  The route of entry of the  infectious fomites may usually
be inferred from the location of the  characteristic lesions.
Further transmission of the disease  may occur from discharges
from these areas.

     There are three common varieties of this organism, var.
hominus, var. bovis, var. avium.  Each has a different patho-
genicity.  The bovine type is progressive and sometimes fatal
in cattle.  Manifestation in horses  is progressive and usually
associated with infection in cattle.   The disease is also pro-
gressive in swine who are highly susceptible and in cats, who
are usually infected from tuberculous milk.  This type rarely
affects sheep, goats, dogs,  humans and does not  affect birds.

     The avian type affects all birds.  Due to their lowered
resistance to disease, disease occurs mostly in  domestic or cap-
tivated, wild birds.  This type produces chronic symptoms in
swine and is progressive in sheep.  It is the most common form
to affect these animals.  Rarely are  goats, horses, cattle,
dogs or cats affected by the avian variety.

     Cattle, swine and cats are resistant to the var. hominus.
This type doesn't affect horses, sheep, goats or most birds.  Only
psittacines are not resistant and their infection is usually asso-
ciated with tuberculous owners.  Dogs may also contract the
disease from their owners and it manifests itself in the pul-
monary form.  Var. hominus is of course, the most common form
to affect man.

-------
     When large animals contract tuberculosis, cattle normally
develop lesions in the lungs and in the cephalic and thoracic
lymph nodes.  Swine develop lesions in the cephalic and abdominal
lymph nodes, and it is sometimes fatal.  In sheep and goats, the
affected areas are the lungs and thoracic lymph nodes.

     In smaller animals, dogs are affected in the thoracic organs
and cats, initially in the abdominal organs and later in the lungs.
Poultry develop the disease slowly.  Initially there is intestinal
ulceration and then necrosis and ulceration of the spleen and
liver.

     Wild animals rarely contract the disease except when associ-
ated with humanity, zoos or cattle raising areas.  Wild birds
commonly develop lesions in the spleen and liver.  However, out-
ward signs of infection are variable and may be non-existent.
Outbreaks among wild animals involving more than one individual,
are usually associated with man.  Infection stems from exposure to
either infected farm animals or sewage outfall.

     Humans are quite susceptible to tuberculous infections but
rarely manifest  tuberculous disease.  Generally the route of
entry determines the site of primary lesions.  Inhalation pro-
duces lesions in lungs and tracheobronchial lymph nodes; inges-
tion:  mouth, tonsils, neck lymph nodes, intestine; skin:  ulcera-
tion at specific site and regional lymph nodes.  After a period
of days the infecting organisms spread to all parts of the body.

     Most of the tubercle bacilli do not find suitable sites for
development.  Some remain microscopic foci and may promote infec-
tion of bones, joints, lungs and other organs as much as ten years
later.   Disease due to reinfection usually becomes the chronic
pulmonary form.  This form is the prime cause for morbidity and
mortality.

     Man may be regarded as the sole carrier.  Animal infection
stems directly and indirectly from man, and any residual foci of
infection in cattle are eliminated through mild pasteurization.
                               98

-------
RESULTS OF TASK IV - INDIRECT EFFECTS

     The effects of cooling device drift are not limited to direct
reactions to and manifestation of disease from toxins and patho-
gens.  The infection of plant and animal and human populations may
either be secondarily transmitted or spread to other members of
the community or may lead to interruption of human and animal food
sources.

     The potential of a given agent to indirectly or secondarily
cause disease in any given population and the insuring patterns
of infection or intoxification relies on the same variables as
discussed under Task III.

     1.   Portal of entry for the disease-causing agent
     2.   Portal of exit
     3.   Incubation period
     4.   Gradient of infection
     5.   Mode of spread
     6.   Survival in nature
     7.   Susceptibility of the population at risk

Essentially all discussion contained in the previous section
holds true and only the exceptions will be noted here.


Portal of Exit

     Under this task, discussion of this facet takes on new impor-
tance.  Organisms excreted from infected individuals, predominantly
by fecal or urinary routes of exit and perhaps respiratory tract
discharges, may find their way quite readily into polluted river
or ground water.  Virtually any organism in any tissue of any sus-
ceptible host, excreted by any route, could be found in water
sources or areas of food cultivation.  The ability of the organism
or disease to survive, multiply or remain virulent in such settings,
determines the potential for its transmission to successive indi-
viduals.


Mode of Spread

     The five conventional modes listed in the previous task are
valid considerations within this section as well.  The difference
lies in the role of vectors as means of transmission.  Vector trans-
mission by definition accounts for one living organism carrying a.
disease to another non-infectious individual.  Vectors act in events
such as insect bites (mosquitos transferring malaria); food chain
transmission (infected plants; low order animals; herbivores; car-
nivores; and predators).  Other instances include contact with
open lesions on infected individuals.

                               99

-------
     Fomites too, play a major role in indirect effects from cool-
ing towers.  Ingest ion of water contaminated with waste from
infected individuals could spread disease.  Plants may harbor
pathogens on its edible parts or concentrate toxins in its leaves,
fruits or roots, as in tuberous plants.  Many small wild animals
concentrate toxins in their fatty tissue, or may simply be in-
•fected with the disease itself.  Inclusion of any of these in a
food supply would further spread the infection or offending agent.
Prior to pasteurization, milk from infected cows was the major
cause of the spread of tuberculosis.

     Concentration of people in close quarters, such as in schools,
encourages person to person transmission.  In recent years winter
bouts of influenza have reached epidemic proportions and there
have been renewed outbreaks of "childhood diseases" (rubella,
chicken pox, measles).  Rapid transmission would also occur be-
tween animals in farm and breeding settings.

Susceptibility of the Populatipn_at Risk

     Susceptibility to an infection may increase when trans-
mitted from one animal, plant or human to a like individual due
to potentiation.   Like individuals are susceptible to like
organisms, varieties, even concentrations or innocula.  As each
individual becomes infected the innocula become more refined to
meet the specifications necessary to infect subsequent indivi-
duals.  Populations are therefore more susceptible to the agent
being transmitted.
                               100

-------
RESULTS OF TASK V - RECOMMENDATIONS
     Discussion of the recommendations based on the results
of this study,  the results of Task V in the scope of the
study, may be found in Section 3,   Recommendations.
                               101

-------
RESULTS OF TASK VI - COMPUTER SIMULATION
A.  Introduction

     The computer simulation is based on a combination of numeri-
cal and Monte Carlo modeling.  Basically, the modeling is done
in three serial parts for a cooling tower and associated ambient
environment that are specified as input parameters.  Part 1 de-
velopes the microbial environment in the cooling towers based on
the data from the consultants.  Part 2 develops and distributes
the aerosols (micro-microbial environment) from the cooling
tower into the environment.  Part 3 calculates the microbial en-
vironment downwind from the cooling tower, evaluates the possi-
bility of infections based on the results of Part 1 and 2, and
prints out results.  Figure 11 is a simplified flow chart for the
simulation.

                            FIGURE  11
                        BASIC FLOW  CHART
                           Determine
                      Microbial Contents
                       of Cooling Tower
                      Develop Plume Model
                                                Meteorological
                                                    Inputs
Isopleths of
Distribution
Organisms
 Probability')
              of
                           Establish
                      Microbial Contents
                           of Plume
                       Stochastic Models
                              of
                           Infection
Isopleths of
 Infection
Probability
as a function
of Distance
                              102

-------
B.  Development of Cooling Tower Microbial Environment

     The consultants have developed a list of pathogenic organ-
ism that can occur in a polluted water source.  This list was
used as a basis to establish the microbial content of the cool-
ing tower.  To do this, a matrix was established listing each
organism from the consultants list versus the following factors:

     1.  ability to produce disease (epidemiological significance)
     2.  survival in surface water
     3.  survival in treated effluent
     4.  survival in cooling device
     5.  survival in aerosols
     6.  integrity in fomites

     For each factor a numerical value between 0 and 5 was
assigned based on the consultants data.  The definitions of
these  numerical constants are listed in Table 18.  Table 19
shows  the matrix and the numerical constants assigned for each
category, for each organism considered.  From the matrix a
weighted product for each organism was found as follows:
                   UJ - IT a&
Where W is the weighted product,  a.^ is the weighting factor
(0 to 1) for the ith factor and fi is the numerical constant
from the matrix.  As a practical  initial matter,  the weighting
factors (a^) were taken as unity,  however, as more information
becomes available and it is possible to refine the model,  this
should be changed.  The products  were grouped, that is,  products
with very similar numerical values were considered to be the
same and normalized.  From these  normalized values a histogram
was constructed and used as the distribution function for  de-
termining the probability that pathogens were present in the
make-up water using Monte Carlo methods to evaluate the  integral
of the distribution function.

     The number of organisms present in the make-up water  (per
unit volume) was modeled somewhat  arbitrarily by  using the
probability from above as the mean of an exponential distribu-
tion limited to 10^ maximum (personal conversation with  Dr.  H.
Freudenthal) .   This was done by a  modified Monte  Carlo simula-
tion.   Thus, the number of pathogens entering the cooling  tower
per unit volume of make up water was established.  This  simula-
tion,  was further modulated by including only those results from
the exponential distribution when  a second selected uniform
random variable exceeded the probability value found earlier.

                               103

-------
                            TABLE 18

       NUMERICAL CONSTANTS USED IN ESTIMATING PROBABLITIES


OCCURRENCE IN POLLUTED WATER SOURCE

(0)  will not occur under any circumstances
(1)  rarely occurs
(2)  compromised - will occur only if concentration or frequency
                   in surrounding environment significantly
                   increases eg. epidemic, leak from toxic
                   substance storage
(3)  will occasionally occur
(4)  will frequently occur
     will always occur
     unknown
SURVIVAL IN A PARTICULAR ENVIRONMENT (SURFACE WATER, TREATED
EFFLUENT, COOLING DEVICE)

(0)  will not survive under any circumstances
(])  rarely survives
(2)  compromised - may survive in this environment only if
                   conditions change eg. type of water treatment,
                   presence of other toxins or pathogens
(3)  will occasionally survive
(4)  will frequently survive
(5)  will always survive
(x)  unknown


EPIDEMIOLOGICAL SIGNIFICANCE

(0)  will never cause disease or direct effects
(1)  rarely causes disease or direct effects
(2)  compromised - host may contract the disease or become
                   affected if its immune system has been
                   weakened
(3)  may cause non-transmittable effects or allergic responses
(4)  usually causes disease
(5)  always causes disease
(x)  unknown
                                104

-------
               TABLE  19




SUMMARY OF PATHOGEN,/TOX IN PROBABILITIES

PATHOGEN /TOXIN
Absidia corymbifera
Absidia ramosa
Actinomyces Israeli
Actinomyces keratolytica
Actinomyces naeslundii
Actinomyces odontolyticus
Actinomyces viscosus
j Arachina propionica
Aspergillus spp.
Aspergillus flavus
Aspergillus nidulans
Aspergillus niveus
Aspergillus restrictus
Aspergillus terreus
Bacillus anthracis
Bacillus cereus
Bacillus subtilis
Bacteriodes fragilis
Bacteriodes melaninogenicus
Basidiobolus haptosporus
Blastomyces dermatitidis
Bordetella parapertussis
Brucella abortus
Brucella canis
Brucella me li tens is
Brucella suis
Candida albicans
Candida guillermondu

produce
disease
4
4
2
2
1
1
1
1
4
3
2
2
2 i
2
4 !
4
2
4 i
4
4 :
1 i
4 !
4 1
i 1
4 !
4 i
2 !
2 1
i i
1 !
occurrence
1
1
3
3
3
3
4
3
4
4
4
4
4
4
1
1
1
1
1
1
3
3
Survival
0) TJ fl
O CD 0)
erf ?H -+J 3
•CH CD C3 rH
k +•> CD HH
3 
-------
                                           (2 of 7)
               TABLE  19




SUMMARY OF PATHOGEN/TOXIN  PROBABILITIES

PATHOGEN/TOXIN
Candida krusei
Candida parapsilosis
Candida pseudotropicalis
Candida stellatoidea
Candida tropicalis
Candida utilis
Candida viswanthii
Candida zeylatoides
Cladosjjprium bantianum
Cladosporium carrionii
Clostridium botulism
Clostridium perfingens
Cocciodioides immitis
Cocynebacterium spp.
Conidiobolus coronatus
Corynebacterium diptheriae
Corynebacterium ulcerans
Cryptococcus neoformans
Dermatophilus congolensis
Enter obacteriae
i
Enterococci
Escherichia coli
Fuscobacterium spp.
Geotrichium candidium
Haemophilus aegyptius
Haemophilus influenzae
Histoplasma capsulatum
Klebsialla pneumonia
	
produce
disease
2
2
2
2
2
2
2
2
1
1
4
3
4
2
1
2 !
j
2
2 i
2
2
4
3 I
4
2
2
2
4
4
occurrence
3
3
3
3
3
3
3
3
1
1
1
4
3
4
1
1
i
3 1
1
1
4
4
4 1
1
4
1
4
4
Survival in
-(->
Q) 73 G hC
0 0) 0) GO)
rf Sn +J 3 -HO

3 OJ In «H O0
CO & -P 0 O T3
4
4
4
4
4
4
4
4
3
3
3
4
3
4
4 ]
3
?
3
4 ;
4 i
5
4
4
4
3
4
1
4
4
1
3
3
3
3
3
3
3
o
Q
3
3
3
3 i
3 !
4
3
3
3
3
1
3
3
4
0
3
0
4
4
i
4
3
4
4
4
5
5
5
3
3
3
5
3
3
3
3
3
3
3
3
!
3
4
4
3
i
4
1
4
4
aeroso-
lization
integrity
in fomites
5
5
5
5
5
5
5
5
5
5
5
4
5
4
5
4
3
4
4
5
5
4
5
5
4
4
I
5
5
5
5
5
5
5
5
3
3
5
5
5
5
4
5
5
5
5
5 }
i
\
4
5
5
5
5
4
5
4
                 106

-------
               TABLE  19




SUMMARY OF PATHOGEN/TOXIN  PROBABILITIES
PATHOGEN/TOXIN
Listeria monocytogenes
Mucor spp.
Mucor pusillus
Mucor ramosissimus
Mycobacterium bpyis
Mvcobacterium chelonei
Mycobacterium fortuitum
Mycobacterium kansasii
Mycobacterium marinum
Mycobacterium scrofulaceum
Mycobacterium simiae
Mycobacterium tuberculosis
Mycobacterium ulcerans
Mycobacterium xenopi
Nocardia asteroides
Nocardia brasiliensis
NocardJa caviae
Peptococcus spp.
Peptostreptococcus spp.
Phialophora dermatitidis
Phialophora gDugerotii
Phialophora richardsiae
Phialophora spinifera
Phialophora verrucosa
Proteus mLrabilis
Prototheca wickerhamii
Prototheca zopfi
Pseudomonas aeruginosa

produce
disease
2
2
2
2
3
3
3
3
3
3
3
3
3
3
2
2
;
4
4 !
1
1
i 1 !
1
i i
4
1
2
2
i
i 	 L
occurrence
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
4
4
1
1
1
1
1
4
1
1
1
Survival in
4~>
0) T3 « bfi
O 0)0) CO)
OS ?-( 4-> 3 -HO
I+H CD rt rH i— l -H
h +•> 0) «H O >
3 ol JH =H O 0)
Cfl £ -(-> 0 U T3
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
/i
4
4
1
1
1
1
1
1
4
4 !
4 i
4
1
1
4
4
1
1
1
i
1
-i
1
4
1
1
1
1
1
1
1
1
1
1
1
3
3
1
1
1
1
1
1
1
1
1
4
4
1
1
1
1
1
4
1
1
1
aeroso-
lization
integrity
in fomites
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
5
5
4
4
4
4
4
4
0
0
4
4
4
4
4
4
4
4
4
4
4
4
5
4
4
5
5
5
5
3
3
3
3
3
5
0
0
4
                   107

-------
                                           (4 of 7)
               TABLE 19




SUMMARY OF PATHOGEN/TOXIN PROBABILITIES
PATHOGEN /TOX IN
Pseudomonas mallei
Pseudoraonas pseudomallei
Rhinocladiella compactum
Rhinocladiella perosoi
Rhizopus arrhizus
Rhizopus oryzae
Salmonella spp.
Salmonella typhi
Shigella spp.
Shigella boydii
Shigella dysenteriae
Shigella flexneri
Shigella sonnei
Sporothrix schenckii
Staphylococcus agalactiae
Staphylococcus aureus
Streptococcus spp.
Streptococcus agalactiae
Streptococcus faecalis
Streptococcus pneumoniae
Streptococcus pyogenes
Toruplopsis glabrata
Vibrio parahemolytica
Yersina enterocolitica
Yersina pestis
Yersina pseudotuberculosis
Zygomycetes
produce
disease
occurrence
4
4
1
1
4
3
4
4
4
4
4
4
4
2
2
4
2
2
4
2
2
2
4
4
j
4
4
2
1
1
1
1
1
1
4
4
4
4
4
4
4
3
1
4
1
1
4
4
1
1
4
4
4
4
1
Survival in
-(->
0) TJ G bfl
O  3 -HO
 0) =H O >
3 ni ^ «H O Q)
ws -p 0) o T3
3
4
1
1
4
4
4
4
4
4
3
3
3
4
4
4
1
1
4
4
4
4
4
4
4
4
1
3
3
1
1
4
4
1
3
1
1
1
1
1
1
1
4
0
0
3
1
1
1
1
1
3
1
1
*_
3
1
1
3
3
4
4
4
4
3
3
3
1
1
4
1
1
4
3
1
1
5
4
3
4
1
aeroso-
lization
integrity
in foraites
4
3
5
5
5
5
4
4
4
4
4
4
4
4
5
5
4
4
4
4
5
5
4
4
4
4
5
5
4
3
3
4
4
5
5
5
3
3
3
3
4
4
5
4
4
5
5
i
4
4
5
5
4
5
4
!
                  108

-------
               TABLE  19




81\ MMARY OF PAT HOGLVTOX 1N PRQBABIL IT IE S


PATHOGEN/TOXIN









Acenapthene
Acetone
Acrolein
Acrylonitrile
Aldrin
Antimony
! Arsenic
Asbestos
i
! Benzene
Benzidene
Beryllium
Biphenyl (Diphenyl)


Cadmium
Carbon Tetrachloride
Chlordane
Chlorinated Benzenes

Chlorinated Ethanes
Chlorinated Napthalene
Chlorine
Chloroform


Clorophenol
Chromium
Copper
Cyanides

DDT & metabolites
Diabyl Ethers
Dichlorobenzenes
Dichlorobenzidine
,„ 	 . 	 , 	 	





CD GJ
o in
3 a
t3 CD
o uj
?H *H
tx-o
3
3
3
4
4
4
4
4

3
4
4
4


3
3
4
1

1
3
4
3


3
i 4
4
4

4 !
4 ;
i 3
3 ;
i. 	 ,i


0)
o
S3

£_!
sx
£j
o
o
0
q
4
4
4
4
3
4
4

4
4
4
3


4
1
4
4

4
4
5
4


1
3
3
3

4

2
3


Sur^



CD
O
Cti 5-i
^ CD
Sx -P
3 ixS
03 S
4
2
1
4
4
4
A
5

4
4
4
4


4
4
4
4

5
4
1
; 4


i
4
4
4

5

< 5
4|
_ J

i/- i v a 1


-H
T5 G
0> CD
-H ;3
G5 ' — 1
CD 4H
?-( 'i -(
-H CD
4
3
4
4
4
4
4
4

5
4
4
4


4
4
4
5

5
4
O
5


2
4
4
4

5

4
4


in



tc
G CD
•H O
rH -H
0 >
O (D
0 T3
4
1
1
1
4
4
: 4
4

1
4
4
4


4
1
4
4

_.
1
4
4
4


4
3
4
4

4

4
4





G
1 O
O -H
K -P
O rf
?-i SI
CD -H
ccj f™^
i
5
5
5
1
1
5
3

5
1
1
1


4
5
3
1

3
1
4
4


3
2
2
. 4

3
1
2
i
	 !


01
>i CD
-H 4~*
•H -H
?H S
bJD O
CD «M
•M
G G
•H -r-l
4
4
1
1
4
5
3
5

4
2
2
4


1
4
4
4 i
!
4 |
2
1
1 '

1
4
5
1
1
,

4
1
4
4
.. .. 	
                 109

-------
                                            (6 of 7)
                TABLE  19


SUMMARY  OF PATHOGEN/TOXIN  PROBABILITIES

PATHOGEN/TOXIN









Dichloroethylene
Dichlorophenol
Dichlor opropane ,
Dichloropropene
Dieldrin
2,4, Dimethyl Phenol
Dinitrotoluene
Diphenylhydrazinr
Endosulf an
Endrin & metabolites
Ethylbenzene
Haloether
Halomethanes
Heptachlor & metabolites
Hexachloro 1,3 Butadiene
Hexachlorocyclohexane
(Lindane)
Isophorone


Lead
Mercury & compounds
Methyl Ethyl Ketone
(Butanone)
Napthalene

Nickel & compounds
Nitrites
Nitrobenzene
Nitrophenol






CD 
O (1)
O T3
4
X

4
4
1
X
4
4
4
4
1
4
5
4

4
4


4
4

2
5
i
4 j
4 i
4
2





c
1 0
O -H
tn -M
o cd
!H N
0) -H
CO rH
1
3

1
3
3
1
1
1
3
2
1
4
3
X

1
3


1
4

3
5

2
4
3
3



tn
>> CD
-p +J
•H -H
s-. a
bC O
0) ^H
4->
c a
•H -H
1
4

4
4
3
1
4
2
4
4
4
4
4
X

4
4

t
1
2 !
;
4
1

4
2
4 !
1
X


                1
                I

               -JL...
                 110
.!		...J

-------
               TABLE 19




SUMMARY OF PATHOGEN/TOXIN PROBABILITIES

PAT HOG EN /TOXIN









Nitrosamines
PCB's
Pent ach lor ophenol
Phenol
Phthalate Esters
Secondary Amines
Selenium & compounds
Silver & compounds
Styrene
Tetrachloroethylene
Thallium & compounds
Toluene

Toxaphene
Vinyl Chloride
Zinc & compounds














-_ „._





0) 0>
o 03
3 a
•o 0)
O ffi
fn -H
0
CJ
ol ^
 CD
+-1 3
Ctf rH
0) 'H
^1 'H
+J 0)
X
5
4
5
X
1
5
4
4
5
4
3

4
4
2









1






in



bfi
c: o>
•H O
rH -rH
0 >
O 1>
u -o
5
4
4
3
4
1
4
4
4
4
4
3

4
4
4



















c
1 O
O -H
03 -p
O rf
^ N
0) -H
Ol rH
5
X
3
4
X
5
3
2
3
X
1
3

1
3
1





-









- - -1 -- • '

0)
>> 0)
-p -p
•H -H
!H S
bfi O
Oi in
-P
d d
•H -H
4
X
4
3
X
1
1
1
1
X
1
4

4
1
X










i






-------
     The value of make up water was estimated as equal to the
sum of the evaporation loss and the droplet losses.  These
losses were calculated on the basis of the cooling tower design
parameters and/or the cooling tower operating condition.  For
the present model, blow-down was assumed to restore the initial
(steady-state) operating condition of the cooling tower periodi-
cally with respect to salt and the pathogen concentration.  The
time between blow downs was calculated as:

                     T=(CONC-1.) TVOL/UMW

Where T is the time between blow downs,  CONG is the maximum
allowable concentration (input parameter), TVOL is the tower
water value and UMW is the make up water input rates.  The total
average value of make up water taken in, then was calculated as:

                     BINVOL = UMW X T/2

where BINVOL is the total amount of water taken in between blow
downs.  This was multiplied by the number of organisms per unit
volume derived earlier to establish the  average microbial content
of the cooling tower.  As can be seen, the microbial content is
then the average number of organisms taken in between blow
downs diluted by the value of water carried by the tower.  In
the present model, no specific capability is included for growth
or death of pathogenic organisms in the  cooling tower.  Their
concentration is determined purely by input conditions and di-
lution factors.  It is also assumed that the droplets are
spherics and are homogeneous samples of the cooling tower micro-
bial environment.  Figure 32 shows a simplified flow chart of
that portion of the simulation relating to the development of
the microbial environment in the cooling tower.
                               112

-------
                           FIGURE 12

               MICROBIAL CONTENT OF COOLING TOWER
potential
organisms
   Monte Carlo Model

of Microbial Community

    in Make Up Water
                         Make Up Water

                             Model
                         Cooling Tower

                      Microbial Community

                          Development
                        to Plume Model
infectious
importance
                              113

-------
C.  Aerosol Model - The Spatial Distribution of Organisms

     The aerosol model that distributes the pathogens in the
environment is based on the ORFAD Model (Oak Ridge fog and
drift) (Wilson, 1975 & LaVerne 1977).  The elements compris-
ing the plume rise, the distribution of the aerosol droplet
sizes and their physical distribution have been developed
from this model.  The model for this portion of the simula-
tion takes cooling tower and environmental parameters (as
inputs) and calculates the plume rise from the cooling tower
and the plume environment.  As in the ORFAD model, the aero-
sols are assumed to travel in ballistic trajectories set by
the rise velocity of the plume centerline and the fall velo-
city of the droplet with respect to the plume centerline.

     Plume rise is calculated as follows:

     1.  Determination of a buoyancy flux parameter (F)
     2.  Calculation of atmospheric stability (S)
     3.  Determination of the plume centerline (trajectory) (PR)
           based on F,  S and wind conditions.

     The flux buoyancy parameter (F) is that developed by Hanna
(ref.  3).   The atmospheric stability parameter is derived from
the Pasquill stability class as determined from weather data
(input parameters) that determine the atmospheric temperature
gradient and the dry and wet bulk temperatures.   Wind conditions
(input parameters) are taken as 1 m/sec minimum,  even for calm
ground conditions since the wind velocity, generally is rarely
less than this at typical tower heights.  The plume is assumed
to be disturbed over a 22.5° angle downwind.  Unlike ORFAD, this
program computes for the downwind condition without regard to
specific geographic direction.   The day is broken into 4 hour
segments starting at 0000 hours,  and wind velocity, temperature
(dry and wet bulb), stability and percent operating capacity
of the plant are inputted for each time segment.   Computations
are done for each four hours and are summarized daily.

     The aerosol drift  deposition is based on ballistic
pie as discussed by Laverne.   This trajectory model
assumes that all particles of a given original size (at the
cooling tower) will fall to the ground at the same distance from
the cooling tower for a given set of wind, stability and humidity
conditions.  This distance is such that the total trajectory is
equal to the local plume height above the ground.

     The distribution of aerosol particle  sizes  within the exit
plume is shown in Figure 13(A).  As in the ORFAD model, this
distribution is recomputed in terms of the cumulative distribu-
tion function to obtain a refined droplet size distribution model
(Figure 13 (B).   Fall velocities are computed by using Stokes
law for particles smaller than about 80 urn and a relationship

                            114

-------
               FIGURE  13
      DROPLET SIZE DISTRIBUTION
0.4''
o
£ 0.3'
d
a4
(D
" n /
l-H U . L
(D
B o./
«j
r-l



























*" 0.0





I

0  100  200   300   400  500   600  700  800
         droplet diameter D
                 (A)
+•> OJ
0) -M
g  0.0024
o a
!H O
Crt 0.0016
•H fH
DO D n n r\ n o*
qo 0.0008
rt C
fi n n n




-





—











._


























—
























i—j

























m





«





-












w





"n
0   100  200  300 400  500  600  700   800
         droplet diameter D (i\m)
                   (B)
                   115

-------
given in Letester (1966) for particles greater than about 80 v^m.
Particles in relative humidity environments greater than 76% are
assumed not to evaporate, while particles in environments with
less than 76% are assumed to evaporate, either to saturation
(76%-50% R.H.) or dryness (R.H.  50%).  Following the methods
outlined in Laverne (1977), particles are distributed downwind
in accordance with wind velocity, plume and weather condition.
From these data, and the microbial input data from part 1 of the
simulation, the total number of organisms impinged per unit area,
per unit time, and present per unit volume (steady state), are
easily calculated.  The flow chart for this phase of the simu-
lation is shown in Figure 14.  It is assumed that the organisms
that find their way into the aerosol will all survive if there
is no evaporation (R. H.  76%) that 50% survive in,the R. H.
range of 76% to 50% and that 20% survive when the R. H. is 50%.
These factors are further modified by 0.2 if there is direct
sunlight (0800-0800) present or 0.5 with cloud cover.  These
factors are rather arbitrarily chosen and should be re-evalu-
ated (perhaps dynamically simulated in further model develop-
ments.  These results (organisms/M^ and organisms/M^) are tabu-
lated for each four hour interval.
                             116

-------
            FIGURE  14
SPATIAL ORGANIZATION OF ORGANISMS
      Input: Cooling Tower

       Microbial Community
       Meteorological Data
               i
           Plume Model

   (Transport, Survivability)
     Spatial Distribution of

       Microbial Community
     Probability of Finding
        a Given Pathogen
         per Unit Volume
        at a Given Distance
                117

-------
 D.  Estimation of Infection Probability

      The previous elements of the simulation have established a
 potential microbial density for distances downwind from the cool-
 ing tower.  It is now necessary to establish the extent to which
 these microbial densities are capable of producing infection.
 Two basic possibilities exist.  In one case, inhalation of
 organisms can create an infection and in another, physical con-
 tact (touching or ingestion) will create an infection.  In
 either case, a threshold level establishes a minimum below which
 nothing will happen.  The estimation of this threshold is ex-
 tremely difficult.  Furthermore, the consequences of infection
 may vary from a general slight malaise to severe symptoms that
 require extensive care.  The model developed here, does not con-
 sider the epidemiological affects of interaction between infected
 and non-infected individuals, but only considers the direct
 effect of the interaction between the microbial environment and
 the individual.  Figure 15 is a simplified flow chart for Part 3
 of the simulation.

      The lifetime of deposited (or airborne) microbial particles
 has (again, rather arbitrarily) been taken to be 24 hours* on
 the average.  The average ventilation value for a population is
 about 20,000 liters per day (Haup).   Therefore, for the air-
 borne organisms,  the average number  of organisms inhaled/day
 is calculated.  A sticky problem is  that of determining the
 number of organisms required to declare an infection.  A ran-
 domly distributed variable,  exponentially distributed with a mean
 of .1 X (3 X 106) was used to establish (in a Monte Carlo loop)
 the basis for estimating the number  of organisms required to
 generate an infection.   The  same approach is used with respect
 to the number of  particles injested  with the assumption that an
 individual will ingest the microbial flora that falls on one
 square meter of surface in 24 hours.   Results for 24 hours are
 obtained from the summation  or averaging (as required) of the
 data accumulated  for each four hours.

      The above data is then  used to  generate infection probabili-
 ties, based on the number of times the infection threshold is
 pierced during the interval  under consideration.   This resultant
 is tabulated as the result for a particular simulation based on
 given input condition.

     *It is recognized that many organisms remain viable for
periods much longer than 24 hours, even years in some cases.  How-
ever, it was assumed that the cooling tower, Figures 16-20, environ-
ment would not favor encystment or spore formation, and therefore,
the cells would be susceptible to die-off due to uv, desiccation,
etc. Twenty-four hours was picked as  a working number for the pur-
pose of modeling.


                               118

-------
                            FIGURE 15
                        INFECTIVITY MODEL
                      Spatial Distribution

                               of

                         Microorganisms
Isopleths:
spatial
distribution
construct
infection
probability
                       Infectivity Models

                      a) Simple Stochastic

                          b) Recurrent
       Output Data

Probability of Infection

  Spatially Distributed
                                119

-------
 E.   Input Data  Requirements

     Table 20 lists  the  input data requirements and lists some
 typical  values  used in  the simulations.  These are printed for
 each simulation as  part of the data file.   Figure 16 shows a
 typical  data  file.   Figures 17-21 shows the result of simulations
 under  various conditions of cooling tower operation and environ-
 ment .

     Figure 17  simulates a typical summer day.  Weather conditions
 represent.the most  significant difference in the simulations.
 Internal parameters on  which the model is based, were previously
 discussed in  Section 2.   The fixed parameters were selected as
 typical  for a large,  natural draft tower.  The day was divided
 into four (4) hour  intervals as shown and the environmental para-
 meters are varied as they might be for a summer day.  The results
 of the simulation for each four hour interval are shown along
 with a summary  of operating parameters for that interval.  Also,
 listed for that  interval are distance from the cooling tower,
 plume  rise, the  average number of organisms per cubic meter of
 air, the average number of organisms landing per square meter of
 surface  area, all based on the output of the simulation.  In
 addition, there  is  the  Monte Carlo derived probability that the
 effluent will contain infectious organisms.  Following the four
 hour listings are summaries for the 24 hour period detailing the
 number of organisms  airborn per cubic meter, their incidence on
 surfaces per  square  meter, and the average 24 hour probability
 of effluent containing  infectious organisms.

     The second  summary  shows the results of exposure of a popu-
 lation to these  infectious organisms with a maximum instantaneous
 susceptible population  of 20%.   The organisms are assumed to be
 ingested from both  airborne particles and those picked up by con-
 tact with surfaces.    This leads to the calculation of the average
 number of particles  ingested assuming the population distributions
 described in the methodology.   These averages are used in a simple
 infection model  to derive the listing of the population fraction
 affected by the  effluent.

     The results support the concept that the potential for
 affecting a downwind  population is present, unless measures are
 taken to control infectious organisms and toxins in the make-up
water and the tower  to  suppress these effects.  As pointed out
 in Section 2, many  assumptions have been made because real data
 does not exist.   These  data,  when available, could be used to
 considerably  strengthen  the model.  However, even in its present
 form, the model  clearly  shows that if infectious organisms are
 present in the cooling  tower environment, they will surely appear
 in the population downwind from the cooling tower.

     Figures  18  and  19  represent the same operating parameters
 and  environmental conditions.   In Figure 18, 20% of the popula-
 tion is susceptible  to  infectious organisms and toxins.  Figure
 19 represents a  worse case situation wherein 100% of the popula-
                              120

-------
tion is affected by transmitted pathogens and toxic particles.
Figure 20 simulates conditions for a tower with a higher  level
of output, cooler environmental temperatures and a population
which is 20% susceptible.  Figure 21 represents a tower operating
at 100% capacity with a 20% susceptible population.

                            TABLE 20
                  A TYPICAL INPUT PARAMETERS
     PARAMETERS                UNITS

Cooling tower height             ft

Tower inside diameter            ft

Temperature range  (in  tower)     °F

Exit air velocity              ft/sec

Drift fraction                   g/g

Wind velocity                    knots

Dry bulb temperature             °F

Wet bulb                         °F

Pasquill Stability class
TYPICAL VALUES

      400

      200

       25

     0-50

 10~4 to 10~6

       50

  -20 to +100

  -20 to +100

     1-6
These data were  inputted  via  a  separated data file called
FOR	DAT.  The  program is  now  set  to  accept  FOR28.DAT.
These files are  shown  with  each output run shown in Figure 6

                             FIGURE  16

                        Typical  Data File
                    TYPE  (FILE)  FOR26.DAT
                    450. ,200. , 5.E-5, 12. , 1.3,25. , 1200.
                    50. ,48. , 10. , .8,4.
                    cr -~t  si er  er    o A
                    •-'.i.'!, m 7 *T-_I . 7 •_' • 7 • •-' J *T •
                    60. ,50. , 20. , 1 . ,5.
                    A3. ,50. ,20. , 1. ,5.
                    58. ,50. , 5. , 1. ,4.
                    54. ,50. , 10. , 1. ,4.
                    3,5,7,9, 13,223
                               121

-------
                                                                 (1 of  9)
                           FIGURE

#**************COGLING TOWER FIXED
TOWER HEIGHT (FEET)
TOWER DIAMETER (FEET)
HEAT LOSS (MEGACAL/SEC.MAX)
TEMPERATURE RANGE  (DEG F)
DRIFT FRACTION (G/G)
CONCENTRATION RATIO  (G/G)
EXIT VELOCITY (FT/SEC)
                      450.OO
                      250.00
                     1000.UO
                       25.00
                    0.O00050
                        1. 30
                       10. OO
           —COOLING TOWER ENVIRONMENTAL PARAMETERS—
TIME (HHS)
   O- 4OO
 400- 800
 800-120O
1200-1600
160O-200O
2000-2400
DKf BULB T
  70.00
  75.00
  80.00
  90.00
  8O.OO
  72.00
WET BULB T
  65.OO
  /O.OO
  72.00
  83. 00
  75.00
  /O.OO
WIND VEL.
  5.OO
 1O.OO
  8. OO
 12.OO
  9. 00
 10. OO
OPER CHP
 .80
 .90
1.00
1.00
l.OO
 .90
STABILIT^
5.
4.
5.
5.
                                    122

-------
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS

PROBABILITY OF EFFLUENT CONTAINING ORGANISMS  .53
HEAT LOSS (MEGACAL/SEC)             800.Ou
DRY BULB TEMPERATURE (DEG F)         7O.OO
WET BULB TEMPERATURE (DEG F)         65.OO
WIND VELOCITY (KNOTS)                 5.OO
                                             0- 400HRS
DISK Ml)
    0. 10
    0. 15
    0.20
    0.30
    0.50
    0.75
    1.00
    1.50
    2.00
    2.50
    3.
    4.
    5.
    7.
    9.00
   10.00
   12.00
   15.00
   20. OO
   25.00
,00
, OO
,00
,OO
SE(M)
271.9
356 . 3
392 . 9
392.9
392.9
392.9
392.9
392.9
392.9
392.9
392.9
392.9
392.9
392.9
392.9
392.9
392.9
392.9
392.9
392.9
ORG/.M3/4HRS
0.0
0 . O
2403. 1
1443.5
1254.4
1689.0
1085.7
1600.5
1100.6
802.7
539.6
340 . 0
24O.6
149.5
116.2
20.2
16.8
13.5
6.9
5.5
ORG/M2/4HRS
0.0
0.0
4753. 7
2186.2
1245.0
1305. 1
630. 1
665.1
378.0
223.3
118.6
57.2
29.7
12.8
10.0
1. 1
0.9
0.7
0.2
0.2
                             123

-------
                                                            (3 of. 9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS  400- SOOHRS
PROBABILITY OF EFFLUENT CONTAINING ORGANISMS
HEAT LOSS (MEGACAL/SEO             900.00
DRY BULB TEMPERATURE (DEG F)         75. OO
WET BULB TEMPERATURE (DEG F)         70.OO
WIND VELOCITY (KNOTS)                10.OO
55
DIST(MI)
0. 10
0. 15
0 . 20
0.30
0.50
0. 75
1 . 00
1.50
2.00
2.50
3.00
4 . 00
5 . OO
7 . 00
9.00
10.00
12.00
1 5 . 00
20.OO
25 . OO
PLUME HISE(M)
136.4
173.7
216.5
283.7
398.8
522.6
633.0
829.5
990.9
990 . 9
990.9
990 . 9
990.9
990.9
990.9
990.9
990.9
990.9
990.9
990.9
ORG/M3/4HRS
0.0
144965.9
38423.6
11914.7
3730.7
1642.6
945.4
445.8
269.6
1636.7
1276.9
1973.4
1370.0
2178.6
1 556 . 4
1278.2
1065.2
687 . 6
433.2
3O6.3
URG/M2/4HRS
O.O
199142. 1
52783. 1
16367.4
5125.0
2256.5
1298.7
612.5
370.3
1821.2
1267.3
1524.9
795. 1
905.3
534.5
355.6
296.3
151. 1
72.9
37.9
                             124

-------
                                                             (4 of 9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS  800-1200HRS

PROBABILITY OF EFFLUENT CONTAINING ORGANISMS .07
HEAT LOSS (MEGACAL/SEC)            1000.00
DRY BULB TEMPERATURE 
-------
                                                             (5 of 9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS 12OO-16OOHRS

PROBABILITY OF EFFLUENT CONTAINING ORGANISMS .16
HEAT LOSS (J1EGACAL/SEC)            10OO.OO
DRY BULB TEMPERATURE (DEG F)         90.00
WET BULB TEMPERATURE (DEG F)         83.00
WIND VELOCITY (KNOTS)                12.00
Di:
f(MI)
0. 10
0. 15
0.20
0.30
0.50
0.75
1.00
1.50
2.00
2.50
3.00
4.00
5.0O
7 . 00
9.00
10.00
12.00
15.00
20.00
25. OO
PLUME RISE(M)
110.4
144.7
175.3
229.7
289.6
28V. 6
289.6
289.6
289.6
289.6
289.6
289.6
289.6
289.6
289.6
289.6
289.6
289.6
289.6
289.6
ORG/M3/4HRS
O.O
34723.7
9731.7
3069. 1
1165.3
3302.9
4145.4
2791.7
1932.2
3O05 . 8
2838.7
1950.3
1421.7
688.2
535.3
426.2
355.2
247.2
35.8
28.6
ORG/M2/4HRS
0.0
47/00.6
13368.5
4216.1
1600.7
3675.2
3644.5
1879. 1
1121.4
1486.5
1179.6
669.8
395.5
115.8
90. 1
52. 7
43.9
21.2
2.0
1.6
                             126

-------
                                                           (6 of 9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS 16UO-20UOHRS

PROBABILITY OF EFFLUENT CONTAINING ORGANISMS .54
HEAT LOSS (MEGACAL/SEC)            1QUO.00
DRY BULB TEMPERATURE (DEG F)         80.00
WET BULB TEMPERATURE (DEG F)         75.00
WIND VELOCITY (KNOTS)                 9.00
DIST(MI)
0. 10
0. 15
0.20
0.30
0.50
0.75
1.00
1.50
2.00
2.50
3.00
4.00
5.00
7 . 00
9.00
10.00
12.00
15.00
20.00
25.00
PLUME RISE(M)
142.9
1 87 . 3
226.9
297.3
307 . 5
307.5
307.5
307.5
307 . 3
307.5
307.5
307.5
307.5
307 . 5
307 . 5
307.5
307 . 5
307.5
307 . 5
307 . 5
ORG/h3/4HRS
0.0
167370.7
47063.3
14858.2
30438.8
33611.3
27496.8
15536.7
25613.6
18759.0
15632.5
8613.3
6890.6
4135.8
2846.5
2229.6
1858.0
287.2
215.4
117.5
ORG/M2/4HRS
O.O
207502.7
58348. 1
1 3420. 9
33869.6
29550.4
21247.2
9017.4
10643.7
6442.4
5368.7
1893. 1
1514.5
696.0
351.9
191.4
159.5
15.8
11.8
3.6
                               127

-------
                                                            (7 of 9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS 2OOO-24OOHRS
PROBABILITY OF EFFLUENT CONTAINING ORGANISMS
HEAT LOSS (MEGACAL/SEC)             900.00
DRY BULB TEMPERATURE  (DEG F)         72.00
WET BULB TEMPERATURE  (DEG F)         70.00
WIND VELOCITY (KNOTS)                10.00
                                 21
DIST(MI)
    O. 10
    0. 15
    0.2O
    0.30
    O.50
    0. /5
    1.00
    1.50
    2.00
    2.50
    3.00
    4.00
    5.0O
    7.00
    9.OO
   10.00
   12.00
   15.00
   20.00
   25.00
PLUME RlbE(M)
        135.7
        177.9
        215.5
        282.3
        311.7
        311.7
        311.7
        311.7
        311.7
        311.7
        311
311
311
311
311
311
311
311
            7
            7
            7
            7
            /
            7
            /
            7
        311.7
ORG/M3/4HRS
0.0
742564.0
203802.9
65920.5
64452.4
173105.7
243932.9
133611.9
62840.0
40217.6
27928.9
3567. 1
2282.9
963. 1
1076.2
918.9
699.0
413.6
268.6
192.2
ORG/M2/4HRS
0.0
0.0
0.0
O.O
184820.3
330926.0
349744.7
127712.9
45049.2
23065.2
13347.9
1278.6
654.6
197.3
171.5
131.7
83.5
39.5
19.3
11.0
                                128

-------
                                                           (8 of 9)
	  	__„	  __	„.„„   '""• /i Lj i~i i i o T i"i T A i '~      __—.	_-.—-__	—	
                  ^^ n'JUrv I U I HL.O  	

DAILY PROBABILITY uF EFFLUEWT CONTAINING  ORGANISMS .34
DIST(MI)
    0. 10
    0. 15
    0.20
    0.30
    0.50
    0.75
    1
    1
      oo
      50
    2.00
    2.50
    3.00
    4.00
    5.00
    7. 00
    9.00
    1O.OO
    12. OO
    15.00
    20.00
    25.00
ORG/M3/DAY
       0. 0
 1107339.0
  311119.8
   98662.0
  105227.0
  215556.6
  280964.0
  155911.4
   94571.7
   66768.6
   50172.2
   17523.1
   12931.0
    8573.2
    6487.0
    5151.9
    4226.6
    1685.0
      986.8
      664.9
ORG/M2/DAY
       0. 0
  454345.4
  129253.4
   4119O.6
  232410.3
  369901.7
  379159.9
  141004.1
   58955.1
   33844.5
   21953.7
    5660.8
    3511.6
    1983
    1202
      756
      604
      230
      107
       54.
                                           8
                                           O
                                           cr

                                          , 1
                                          ,4
                                          ,6
                                129

-------
                                                             (9  of  9)
              SUMMARY UP RESULTS
DIST(MI)
 0. 10
 0. 15
 0.20
 0. 30
 0.50
 0.75
 1.00
 1.30
 2.00
 2.50
 3.00
 4.00
 5.0O
 7.00
 9.00
10.00
12.00
15.00
20. OO
25.00
AVG NO. PART.
  INGESTED/IND.

         0. 0
   1561664.4
    440373.2
    139852.6
    337637.3
    585458.3
    660123.9
    296915.5
    153526.8
    10O613.1
     72125.9
     23183.9
     16442.5
     10557.1
      7689.0
      3908.4
      4830.7
      1915.4
      1094.5
       719.6
                                 PERCENT
                                      BY
AFFECTED
EFFLUENT
  0
 18
  0
  1
 20
  9
 13
 18
 1 1
 1 0
  5
  0
  1
  0
  O
  O
  0
  O
  0
                                               000
                                               135
                                               0OO
                                               244
                                               324
                                               221
                                               626
                                               064
                                               507
                                               486
                                               914
                                               318
                                               OO6
                                               OOO
                                               OOO
                                               OOO
                                               409
                                              0.215
                             130

-------
                                                            (1 of 9)
                              FIGURE 18
                       TOWER FIXED PARAMETERS***************
TOWER HEIGHT (FEET)
TOWER DIAMETER  (FEET)
HEAT LOSS (MEGACAL/SEC,MAX)
TEMPERATURE RANGE  (DEG F)
DRIFT FRACTION  (G/G)
CONCENTRATION RATIO  (G/G)
EXIT VELOCITY (FT/SEC)
                      450.00
                      200.OO
                      750.00
                       30. 00
                    0.OO0050
                        1.40
                       10.00
           —COOLING TOWER OPERATING PARAMETERS—
TIME (HRS)
   0- 400
 400- 800
 SOO-1200
1200-1600
1600-2000
2000-2400
DRY BULtf
  72.00
  70.00
  72.00
  75.00
  80.00
  77.00
WEI" BULtf
  68.00
  66.00
  67.00
  71.00
  75.00
  74.00
WIND VEL.
  5. 00
  2.00
  3.00
  5. 00
  8. 00
  5.00
OPER CAP
1. 00
1. 00
 . 70
1. 00
1. 00
1. 00
STABILITY
3.
2.
2.
3.
3.
4.
                                    131

-------
                                                            (2 oi 9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETER;

PROBABILITY OF EFFLUENT CONTAINING ORGANISMS  .
HEAT LOSS  (MEGACAL/SEC)             750.00
DRV BULB TEMPERATURE (DEO F)          72.00
WET BULB TEMPERATURE (DEC F)          68.00
WIND VELOCITY 
-------
                                                           (3 of 9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS  400- 300HRS
PROBABILITY OF EFFLUENT CONTAINING ORGANISMS
HEAT LOSS 
-------
                                                            (4 of-9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS  800-1200HRS
PROBABILITY OF EFFLUENT CONTAINING ORGANISMS
.'(EAT LOSS (MEGACAL/SEC)             525.00
DRY BULB TEMPERATURE  (DEG F>         72.00
WET BULB TEMPERATURE  (DEG F)         67.00
WIND VELOCITY (KNOTS)                 3.00
22
DIST(MI)
    0. 10
    0. 15
    0.20
    0.30
    0. 50
    0.75
    1.00
    1.50
    2. OO
    2.50
    3. 00
    4.00
    5.00
    7.00
    9.OO
   10.00
   12.00
   15.00
   20.00
   25.00
PLUME RISE(M)
418.6
548.6
664.6
870.8
1224. 1
1604.1
1943.2
2516.3
2847.7
2847.7
2847.7
2847.7
2847.7
2847.7
2847.7
2847.7
2847.7
2847.7
2847.7
2847.7
ORG/M3/4HRS
182550.8
27089.0
11994. 1
4625.9
8503. 4
4034.8
2357.0
1421.7
1748. 1
1249.5
1947.8
1527.9
2239.0
1677.0
1191.0
1071.9
720.9
484.4
321.0
223.0
ORG/M2/4HRS
0.0
0.0
0.0
0.0
16821.0
7330. 1
3917.9
2153.2
2167.2
1240. 1
1712.4
1028.5
1107.3
575.9
331.3
298.2
158.5
81.5
39.7
19. 1
                             134

-------
                                                             (5 of 9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS 12OO-16UOHRS

PROBABILITY OF EFFLUENT CONTAINING ORGANISMS .33
HEAT LOSS (MEGACAL/SEC)             325.00
DRY BULB TEMPERATURE (DEG F)         75.00
WET BULB TEMPERATURE (DEG F)         71.00
WIND VELOCITY  (KNOTS)                 5.00
DISK MI)
0. 10
0. 15
0.20
0.30
0.5O
0.75
1 . OO
1.50
2.OO
2.50
3.00
4.00
5.0O
7.00
9.00
10.00
12.00
15.00
20. 00
25. 00
PLUME RISE(M)
245.7
321.9
390.0
511.0
718.4
94 1 . 3
1140.3
1494.3
1641.8
1641.8
1 64 1 . 8
1 64 1 . 8
1641.8
1641.8
1 64 1 . 8
1641.8
1641.8
1641.8
1 64 1 . 8
1641.8
URG/M3/4HRS
230699.0
37656.7
16870.9
6554. 1
2293.3
1055.7
620.4
298.6
1629.6
2278.6
2111. 1
1377.7
2158.1
1613.0
1145.0
83 1 . 6
693.0
465.7
308.7
214.6
ORG/M2/4HRS
316915. 1
51729.6
23175.8
9003. 4
3150.3
1450.2
852.2
410.2
1813.3
2003.3
1631.3
799.6
1067.2
554 . 0
318.5
182.8
152.3
78.4
38.2
18.4
                               135

-------
                                                            (6 of 9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS 1600-2000HRS

PROBABILITY OF EFFLUENT CONTAINING ORGANISMS .54
HEAT LOSS (MEGACAL/SEC)             525.00
DRY BULB TEMPERATURE (DEG F)         80.00
WET BULB TEMPERATURE (DEG F)         75.OO
WIND VELOCITY (KNOTS)                 8.00
D 1ST (MI) PLUME RISE(M)
0. 10
0.15
0.20
0.30
O.50
0.75
1 . 00
1 . 50
2.00
2.50
3.00
4.00
5.00
7.00
9.00
10.00
12.00
15.00
20.00
25.00
148.5
194.6
235.7
308.8
434. 1
568.9
689. 1
903.0
965.8
965.8
965.8
965.8
965.8
965.8
965.8
965.8
965.8
965.8
965.8
965.8
GRG/ M3/ 4 HHS GRG/ M2/ 4 MRS
1462108.5
615833.1
172227.6
54280.5
17112.9
7553.8
4352.6
2055.0
10592.2
14689.5
13568.4
8817.5
13793.2
10293.2
7304.5
5304 . 7
4420.6
2970.5
1969.5
1369.3
0.0
763497.2
213524.2
67295.8
21216.2
9365. 1
5396.3
2547.7
11786.0
12914.7
10484.5
5117.6
6821.2
3535.0
2032.0
1165.9
971.6
499.9
243.5
117.6
                              136

-------
                                                            (7  of 3)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS 2000-2400HRS
PROBABILITY OF EFFLUENT CONTAINING ORGANISMS
HEAT LOSS (MEGACAL/SEC)             525.OO
DRY BULB TEMPERATURE  (DEG F)         77.00
WET BULB TEMPERATURE  (DEG F)         74.00
WIND VELOCITY (KNOTS)                 5.00
                                           11
DIST(MI)
    0. 10
    0. 15
    0.20
    0.30
    0.5O
    0.75
    1 .00
    1. 50
    2.00
    2. 50
    3.00
    4. 00
          PLUME
    5.
    7.
   00
   00
 9.00
10.00
12.00
15.00
20. OO
25.00
RISE(M)
240.7
315.4
382 . 1
500.7
703.9
922.3
1117.3
1464. 1
1582.6
1 582 . 6
1582.6
1582.6
1582.6
1582.6
1582.6
1582.6
1582.6
1582.6
1582.6
1582.6
GRG/M3/4HRS
52706921 . 0
22199903. 0
6208559.4
1956733. 1
1 1 6004 1 . 0
1011519.6
1120061.6
547435.4
1343405.2
1462743.0
905710. 1
477745.8
305757.3
1 55998 . 6
21427.3
17356. 1
9966. 6
6378.6
7138.4
4223.7
ORG/M2/4HRS
0 . 0
0 . 0
0 . 0
0 . 0
3116082.4
2281934.3
2243878. 1
933247. 5
1844744.9
1 606894 . 3
829138.8
323016.9
167944.6
6 1 204 . 3
6538.6
4766.6
2281.0
1167.9
980.2
464.0
                              137

-------
                                                       (8 of 9)
                        1* Ti'VT" AI O  «._--_. — — __—-*__«.__._«-«.
                          | fj | HL.O


DAILY PROBABILITY OF EFFLUENT CONTAINING ORGANISMS  .3t
DISK MI)
    O. 10
    0. 15
    0.20
    0.30
    0.50
    0.75
    1. 00
    1. 50
    2.00
    2.50
      OO
      00
    5.00
    7. 00
    9.00
   10.00
   12.00
   15.00
   20.00
   25.00
    3.
    4.
ORG/M3/DAY
56158319.0
23114353.0
 6513201.9
 2062131.1
 1201866.5
 1041657.6
 1132778.7
  580027.9
 1378124.3
 1495712.0
  945928.8
  507126.7
  346360.0
  190595.2
   44783-8
   36908.7
   24103.2
   15877.0
   13434.6
    8599.9
ORG/M2/DAY
 2070021.5
 1075373.0
  351883.8
  120723.7
 3172749.3
 233O441.9
 2262998.0
  978581.2
 1886996.8
 1638184.5
  862828.9
  346848.4
  188328.0
   74601.3
   13035.9
    9847.b
    5388.1
    2766.3
    1758.7
     839.7
                            138

-------
                                                     (9  of  9)
               SUMMARY OF RESULTS
DISK MI)
   0. 10
   0. 15
   0.20
   0.30
   0.50
   0.75
   1 . 00
   1.50
   2. OO
   2.50
   3.00
   4.00
   5.00
   7.00
   9.00
  10.00
     00
     00
12
15
20.00
25.00
AVG NO. PAR!.
  INGESTED/IND.

  58228341.0
  24189726.0
   6865085.7
   2182854.8
PERCENT
     BY
                                          API- EC TED
                                          EFFLUENT
   4374615.7
   3372099.4
   3395776.8
   1558609.1
   3265121.2
   3133896.4
   1808757.8
    853975
    534688
    265196
     57819
     46756.2
     29491.3
     18643.3
     15193.2
      9439.6
                                           20
                                           18
                                            6
                                           12
                                           20
                                            3
                                            7
                                           20
                                           18
                                           20
                                            9
                                            6
                                           1 4
                                            1
            000
            613
            878
            173
            000
            674
            069
            000
            308
            000
            432
            754
            787
            795
            351
          1. 133
          0 . 325
          0. 112
          0.420
          0.312
                            139

-------
                                                             (1  of  9)
                          FIGURE 19
*##*###****#**#COOLING TOWER FIXED PARAMETERS***************
TOWER HEIGHT (FEET)
TOWER DIAMETER (FEET)
HEAT LOSS (MEGACAL/SEC.MAX)
TEMPERATURE RANGE  (DEC F)
DRIFT FRACTION (G/G)
CONCENTRATION RATIO  (G/G)
EXIT VELOCITY (FT/SEC)
                      450.00
                      200.00
                      750.00
                       30. 00
                    0.000050
                        1. 40
                       10. 00
           —COOLING TOWER OPERATING PARAMETERS—
TIME (HRS)
   0- 400
 400- 800
 800-1200
1200-1600
1600-2000
2000-2400
DRY BULB
  72.00
  70.00
  72.00
  75.00
  80.00
  77.00
WET BULB T
  68.00
  66.00
  67.00
  71.00
  75.00
  74.00
WIND VEL.
  5.0O
  2.00
  3. OO
  5.00
  8. 00
  5.00
OPER CAP
1. OO
1. 00
 . 70
1. OO
1. OO
STABILITY
3.
•~§
•-.:•
3.
1. 00
                                    140

-------
                                              (2 of 9)
    COOLING TOWER AND  ENVIRONMENTAL  PARAMETERS

PROBABILITY OF EFFLUENT  CONTAINING ORGANISMS
HEAT LOSS  (MEGACAL/SEC)              750.00
DRY BULB TEMPERATURE  (DEG  F)          72.00
WET BULB TEMPERATURE  (DEG  F)          68.00
WIND VELOCITY  (KNOTS)                  5.00
                                      0-  400HRS
D1ST(MI)
    0. 10
    0. 15
    0. 2O
    0. 3O
    0.50
    0.75
    1. 00
    1. 50
    2.00
    2.50
    3.00
    4.00
    5.00
    7.00
    9.00
   10. 00
   12.00
   15.00
   20.00
   25. 00
PLUME
RISE(M)
  257.6
  337.6
  409.
  535.
  753.
  987.
 1195.
 1566.
 1788.
 1 788.
 1788.
 1788.
 1788.
 1788.
 17yy.
 1788.
 1783.
 1788.
 1788.
 1788.
            0
            9
            3
            1
            8
            9
            3
            3
            3
ORG/M3/4HRS
  1157530.7
   171767.8
    76052.8
    29332.3
    10220.6
     4696.3
     2757.4
    24301.0
    15067.9
    10665.2
    16563.4
    12922.4
    18873.4
    15355.8
    10019.8
     9017.8
     6064.6
     4074.8
     2700.9
     1877.2
ORG/M2/4HRS
  1758106.5
   260146.2
   115183.7
    44424.5
    15479.3
     7112.7
     4176.2
    33382.8
    18680.9
    10585.3
    14562.2
     8698.3
     9J33.6
     6381.1
     2787.3
     2508.6
     1333.0
      685. 7
      333.9
      161.2
                 141

-------
                                                           (3 of 9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS  400- 800HRS
PROBABILITY OF EFFLUENT CONTAINING ORGANISMS
HEAT LOSS (MEGACAL/SEC)             730.00
DRY BULB TEMPERATURE (DEG F)         70.OO
WET BULB TEMPERATURE (DEG F)         66.00
WIND VELOCITY (KNOTS)                 2.00
,51
DISK MI)
0. 10
0. 15
0.20
0.30
0.50
0.75
1 . 00
1 . 50
2.00
2. 50
3.00
4.00
5.00
7.00
9.00
1 0 . 00
12.00
15.00
20 . 00
25.00
PLUME RISE(M)
653.5
856.4
1037.4
1359.4
1910.9
2504.0
3033. 4
3974.9
4589.6
4589.6
4589.6
4589.6
4589.6
4589.6
4589.6
4589.6
4589.6
4589.6
4589.6
4589.6
ORG/M3/4HRS
418509.4
62103.3
27497.2
10605.2
3695.3
12797.4
2629.6
4516.2
5681.4
4086.2
6028.0
4735.4
3539. 1
5657.6
3696. 1
3326.5
2237.4
1503.2
996. 1
692. 1
ORG/M2/4HRS
0.0
0.0
0.0
0.0
0.0
23249.6
4777.4
6839.8
7804.6
4546.7
5299. 7
3187.5
2054 . 1
2351.0
1028.2
925.4
491.8
253.0
123.2
59.4
                              142

-------
                                                            (4 oi 9)
    COOLING TOWER AND ENVIRONMENTAL  PARAMETERS  800-1200HRS

PROBABILITY OF EFFLUENT  CONTAINING ORGANISMS .22
HEAT LOSS  (MEGACAL/SEC)              525.00
DRY BULB TEMPERATURE  (DEG  F)          72.00
WET BULB TEMPERATURE  (DEG  f-)          67.00
WIND VELOCITY  (KNOTS)                  3.00
D1ST(MI)
    0. 10
    0. 15
    0.20
    0.30
    O.50
    0.75
    1. 00
    1. 50
    2.00
    2.50
    3.00
    4.00
    5.00
    7.00
    9. 00
   10. 00
   12.0O
   15.00
   20.00
   25.00
PLUME RISE(M)
        418.6
        548.6
        664.6
        870.8
       1224.1
       1604.1
      - 1943.2
       2546.3
       2847.7
       2847.7
       2847.7
       2847.7
       2847.7
       2847.7
       2847.7
       2847.7
       2847.7
       2847.7
       2847.7
       2847.7
ORG/M3/4HRS
182550.8
27089.0
11994. 1
4625.9
8503.4
4034.8
2357.0
1421.7
1748. 1
1249.5
1947.8
1527.9
2239.0
1677.0
1191.0
1071.9
720.9
484 . 4
321.0
223.0
ORG/M2/4HRS
0 . 0
0 . 0
0 . 0
0.0
16821.0
7330. 1
3917.9
2153.2
2167.2
1240. 1
1712.4
1028.5
1107.3
575.9
331.3
29W.2
158.5
81.5
39. 7
19. 1
                               143

-------
                                                          (5 of 9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS 1200-1600HRS

PROBABILITY OF EFFLUENT CONTAINING ORGANISMS .33
HEAT LOSS (MEGACAL/SEC)             525.00
DRY BULB TEMPERATURE (DEG F)         75.00
WET BULB TEMPERATURE (DEG F)         71.00
WIND VELOCITY (KNOTS)                 5.00
DIST(MI)
0. 10
0. 15
0.20
0. 30
0.50
0.75
1 . 00
1 . 50
2.00
2.50
3.00
4 . 00
5 . 00
7.00
9.OO
10.00
12.00
15.00
20.00
25 . OO
PLUME RISE(M)
245.7
321.9
390. 0
51 1 . 0
718.4
941.3
1140.3
1494.3
1641.8
1641.8
1 64 1 . 8
1641.8
1641.8
1641.8
1641.8
1641.8
1641.8
1641.8
1641.8
1641.8
ORG/M3/4HRS
230699.0
37656.7
16870.9
6554. 1
2293.3
1055.7
620.4
298.6
1629.6
2278.6
2111. 1
1377.7
2158. 1
1613.0
1145.0
831.6
693.0
465. 7
308.7
214.6
ORG/M2/4HRS
316915. 1
51729.6
23175.8
9O03 . 4
3150.3
1450.2
852.2
410.2
1813.3
'2003.3
1631.3
799.6
1067.2
554 . 0
318.5
182.8
152.3
78.4
38.2
18.4
                               144

-------
                                                              (6  of  9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS  1600-2000HRS
PROBABILITY OF EFFLUENT CONTAINING ORGANISMS
HEAT LOSS (MEGACAL/SEC)             525.00
DRY BULB TEMPERATURE  (DEO F)         30.00
WET BULB TEMPERATURE  (DEG F)         75.00
WIND VELOCITY (KNOTS)                 8.00
                                               54
DISK MI)
    0. 10
    0 . 1 5
    0.20
    0.30
    0.50
    0.75
    1 . 00
    1 . 50
    2.00
    2.50
    3 . 00
    4 . 00
      00
      00
      00
    7
    9
   1 0 . OO
   12.00
   15.00
   20.00
   25.00
PLUME RISE
-------
                                                             (7 of 9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS  2000-2400HRS
PROBABILITY OF EFFLUENT CONTAINING ORGANISMS
HEAT LOSS (MEGACAL/SEC)             525.00
DRY BULB TEMPERATURE  (DEG F)          77.00
WET BULB TEMPERATURE  (DEG F)          74.00
WIND VELOCITY (KNOTS)                 5.00
              11
DIST(MI)
    0. 10
    0. 15
    0.20
    0.30
    0.50
    0.75
    1.00
    1.50
    2.00
    2.50
    3.00
    4.00
    5.00
    7.00
    9.00
   10. OO
   12.00
   15.00
   20. OO
   25.00
             PLUME
RISE(M)
  240.7
  315.
  382.
  500.
  703.
  922.
 1117.
 1464.
 1582.
 1582.
 1582.6
 1582.6
 1582.6
 1582.6
 1582.6
 1582.6
 1582.6
 1582.6
 1582.6
 1582.6
                          4
                         , 1
                          7
                         ,9
                         ,3
                         ,3
                         ,1
                          6
                         ,6
ORG/M3/4HRS
 52706921.0
 22199903.0
  6208559.4
  1956733.1
  1160041
  1011519
  1120061
   547435
  1343405
  1462743
   905710
   477745
   305757
   155998.6
    21427.
    17356. 1
     9966
     6378
     7138
     4223.7
:S
0
0
4
1
0
6
6
4
2
0
1
8
'.3
6
3
1
6
6
4
ORG/M2/4HRS
0.0
0.0
0.0
0.0
3116082.4
2281934.3
2243878. 1
933247.5
1844744.9
1606894.3
829138.8
328016.9
167944.6
61204.3
6538.6
4766. 6
228 l.O
1167.9
980. 2
                                                        464.0
                              146

-------
                                                         (8 of 9)
                 24  HOUR  TOTALS
DAILY PROBABILITY OF EFFLUENT  CONTAINING ORGANISMS
DISK MI)
    0. 10
    0. 15
    0. 20
    0. 30
    0.50
    0.75
    1. 00
    1. 50
    2.00
    2. 50
    3.00
    4. 00
    5.00
    7.00
    9.00
   10.00
   12.00
   15.00
   20. OO
   25.00
ORG/M3/DAY
56158319.0
23114353.0
 6513201.9
 2062131.1
 1201866.5
 1041657.6
 1132778.7
  580027.9
 1378124.3
 1495712.0
  945928.8
  507126.7
  346360.0
  190595.2
   44783.3
   36908.7
   24103.2
   15877.0
   13434.6
    8599.9
ORG/M2/DAY
 2070021.5
 1075373.0
  351883.8
  12O723.7
 3172749.3
 233044J.9
 2262998.0
  978581.2
 1886996.8
 1638184.5
  862828.9
  346848.4
  188328.0
   74601.3
   13035-9
    9847.5
    5388.1
    2766.3
    1758.7
     839. 7
                             147

-------
                                                      (9 of 9)
              SUMMARY OF RESULTS
DISKMI)       AVG NO. PART.       PERCENT  AFFECTED
                 INGESTED/INO.          BY  EFFLUENT

   O.10          58228341.0                 1OO.000
   0.15          24189726.0                 93.O64
   0. 20           6865085.7                 34.390
   O.30           2182854.8                 60.866
   O.50           4374615.7                 100.000
   O.75           3372099.4                 18.371
   1.OO           3395776.S                 35.344
   1.50           1558609,1                 100.OOO
   2.00           3265121.2                 91.542
   2.50           3133896.4                 100.OOO
   3.OO           1808757.8                 47.158
   4.00            853975.1                 33.772
   5.00            534688.0                 28.933
   7.00            265196.5                 73.977
   9.00             57819.7                   6.753
  10. 00             -46756.2                   5. 667
  12.00             29491.3                   1.625
  15.00             18643.3                   0.561
  20.00             15193.2                   2.101
  25.00               9439.6                   1.559
                           148

-------
                                                           (1 of 9)

                          FIGURE 20

###*****##*«###i;::GOLINGi TOWER FIXED PARAMETERS***********-****
TOWER HEIGHT (FEET)
TOWER DIAMETER  (FEET)
HEAT LOSS (MEGACAL/SEC,MAX)
TEMPERATURE RANGE  (DEG F)
DRIFT FRACTION  (G/G)
CONCENTRATION RATIO  (G/G)
EXIT VELOCITY (FT/SEC)
                      450.00
                      200.00
                     1200.00
                       25.00
                    0.000050
                        1. 30
                       12.00
           —COOLING TOWER OPERATING PARAMETERS-
TIME (HRS)
   0- 400
 400- 800
 800-1200
1200-1600
1600-2000
2000-2400
DRY BULB
  50.00
  52.00
  60.00
  63.00
  58.00
  54.00
WET BULB
  48.00
  45.00
  50.00
  50.00
  50.00
  50.00
WIND VEL.
 10.00
  5.00
 20.00
 20.00
  5. OO
 10.00
OPER CAP
 .80
 .80
1. OO
1.00
1.00
1.00
STABILITY
4.
4.
5.
5.
4.
4.
                                     149

-------
                                                             (2  of-9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS

PROBABILITY OF EFFLUENT CONTAINING ORGANISMS  .S
HEAT LOSS (MEGACAL/SEC)             960.00
DRY BULB TEMPERATURE (DEG F)         50.00
WET BULB TEMPERATURE 
-------
                                                             (3 of-9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS  400- 800HRS

PROBABILITY OF EFFLUENT CONTAINING ORGANISMS .44
HEAT LOSS (MEGACAL/SEC)             768.00
DRY BULB TEMPERATURE (DEG F)         52.00
WET BULB TEMPERATURE (DEG F)         45.00
WIND VELOCITY (KNOTS)                 5.00
DISK MI)
0. 10
0. 15
0.20
0.3O
0.50
0.75
1 . 00
1 . 50
2.00
2.50
3.00
4.00
5.00
7.00
9.00
10.00
12.00
15.00
20.00
25.00
PLUME RISE(M)
287.4
376.6
456.3
597.9
840.4
1101.3
1334. 1
1748. 1
2117.7
2177.6
2177.6
2177.6
2177.6
2177.6
2177.6
2177.6
2177.6
2177.6
2177.6
2177.6
ORG/M3/4HRS
0.0
0 . 0
0.0
0.0
0.0
6755. 1
4638.8
3851.8
4261.7
6670. 4
12485.0
785. 1
1390.9
896.2
1466.0
1240.0
967.3
1737.2
1199.8
877.3
                                                 ORG/M2/4HRS
                                                         0.0
                                                         0.0
                                                         0.0
                                                         0.0
                                                         0.0
                                                         0.0
                                                         0.0
                                                         0.0
                                                         0.0
                                                         0.0
                                                         0.0
                                                       647.6
                                                       855.8
                                                       441.4
                                                       562.2
                                                       414.3
                                                       278.6
                                                       358.3
                                                       204.5
                                                       121.1
                             151

-------
                                                             (4 of 9)
    COOLING TOWER AND ENVIRONMENTAL  PARAMETERS  800-1200HRS
PROBABILITY OF EFFLUENT CONTAINING  ORGANISMS
HEAT LOSS  (MEGACAL/SEC)              768.00
DRY BULB TEMPERATURE  (DEG F)          60.00
WET BULB TEMPERATURE  (DEG F)          50.00
WIND VELOCITY (KNOTS)                 20.00
                                  26
DIST(MI)
    0. 10
    0.15
    0.20
    0.30
    0.50
    0.75
    1. 00
    1.50
    2.00
    2.50
    3.00
    4.00
    5.00
    7.00
    9.00
   10.00
   12.00
   15.00
   20.00
   25.00
PLUME RISE(M)
         67.4
         88.3
        107.0
        140.2
        197. 1
        243.9
        243.9
        243.9
        243.9
        243.9
        243.9
        243.9
        243.9
        243.9
        243.9
        243.9
        243.9
        243.9
        243.9
        243.9
ORG/M3/4HRS
        0.0
        0.0
        0.0
     2930.3
      504.6
      212.0
      159.0
      484.0
      703.4
      507.8
      401.2
      565. 1
      476.8
      319.4
      560.2
      504. 2
      387.9
      284.4
      172.7
      116.4
ORG/M2/4HRS
        0.0
        0. 0
        0. 0
     2642.1
      455.0
      191.2
      143.4
      363.8
      479.6
      280.4
      197.6
      246.6
      159.3
       92.0
      115.5
      104.0
       66. 1
       39.3
       18.8
        9.7
                               152

-------
                                                             (5 oi. 9)
    CODLING TOWER AND ENVIRONMENTAL PARAMETERS  1200-1600HRS
PROBABILITY OF EFFLUENT CONTAINING ORGANISMS
HEAT LOSS (MEGACAL/SEC)              768.00
DRY BULB TEMPERATURE  (DEG F)          63.00
WET BULB TEMPERATURE  (DEG F)          50.00
WIND VELOCITY  (KNOTS)                 20.00
                                               6
DIST(MI)
    0. 10
    0. 15
    0.20
    0.30
    0.50
    0.75
    1
    1
             PLUME RISE(M)
      00
      50
    4
    2.00
    2.50
    3.00
      OO
      00
    7.00
    9 . OO
   10.00
   12.00
   15.00
   20.00
   25 . 00
 66.
 86.
1 04 .
137.
193.
239.
239.
239.
239.
239.
239.6
239.6
239.
239.
1
6
9
5
3
6
6
6
6
6
                          6
                          6
                      239,6
                      239.6
                      239.6
                      239.6
                      239.6
                      239.6
ORG/M3/4HRS
0 . 0
12773.5
4682.3
1635.0
540.3
262.3
196.7
687.9
1190.6
905.7
716.8
957 . 3
858.2
540. 7
947.5
852.8
655.8
480.5
291.7
196.4
ORG/M2/4HRS
0.0
12540.5
4596.9
1605.2
530.4
257.5
193.1
567.5
811.7
557.3
395.8
417. 7
329. 1
155.7
195.4
175.9
111.8
66.3
31.8
16.4
                              153

-------
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS  1600-2000HRS
PROBABILITY OF EFFLUENT CONTAINING ORGANISMS
HEAT LOSS (MEGACAL/SEC)             768.00
DRY BULB TEMPERATURE  (DEG F)         5S.OO
WET BULB TEMPERATURE  (DEG F)         50.00
WIND VELOCITY (KNOTS)                 5.00
                                 29
                                                              (6  of  9)
DIST(MI)
    0. 10
    0. 15
    0.20
    0.30
    0.50
    0.75
    1. 00
    1. 50
    2. 00
    2.50
    3.00
    4. OO
    5.00
    7.00
    9.00
   10.00
   12.00
   15.00
   20.00
   25.00
PLUME
RISE(M)
278.8
365.4
442.6
580.0
815.3
1068.3
1294. 1
1695.8
2054.3
2061.7
206 1 . 7
2061.7
206 1 . 7
206 1 . 7
206 1 . 7
2061.7
206 1 . 7
2061.7
206 1 . 7
2061.7
ORG/M3/4HRS
0.0
15757.6
5776.2
2016.9
666.5
323.5
242.6
848.7
1468.7
1117.3
320. 1
263.3
390 . 5
472.4
386 . 8
348. 1
533.8
487.7
336. 9
246.3
ORG/M2/4HRS
0.0
0.0
0.0
0.0
0.0
0. 0
0.0
0.0
0.0
0.0
314.3
197.9
240.2
206. 1
129.2
116.3
131.0
100.6
57.4
34 . 0
                              154

-------
                                                              (7  of -9)
    COOLING TOWER  AND  ENVIRONMENTAL  PARAMETERS 2000-2400HRS
PROBABILITY OF EFFLUENT  CONTAINING ORGANISMS
HEAT LOSS  (MEGACAL/SEC)              768.00
DRY BULB TEMPERATURE  (DEG F)          54.00
WET BULB TEMPERATURE  (DEG F)          50.00
WIND VELOCITY  (KNOTS)                 10.00
                                 01
DISK MI)
    0. 10
    0. 15
    0.20
    0.30
    0.50
    0.75
    1.00
    1.50
    2.00
    2.50
    3.00
    4.00
    5.00
    7.00
    9.00
   10.00
   12.00
   15.00
   20.00
   25. 00
PLUME RISE(M)
        142.5
        186.8
        226.2
        296.5
        416.7
        546. 1
        661.5
        866.9
       1050.1
       1072.7
       1072.7
       1072.7
       1072.7
       1072.7
       1072.7
       1072.7
       1072.7
       1072.7
       1072.7
       1072.7
ORG/M3/4HRS
   667715.0
    93190.5
    40975.7
    15737.4
     5470.4
     2511.0
     1473.6
     6882.1
     5025.6
     7100.4
     5563.2
     8119.3
     6069.6
     7910.5
     6439.5
     5795.5
     4408.2
     2845.9
     1792.7
     1267.5
ORG/M2/4HRS
  1213064.7
   169303.0
    74442.2
    28590.8
     9938.3
     4561.9
     2677.2
    11439.3
    . 7611.4
     8803.0
     6190.2
     6273.9
     4085.6
     3912.1
     2211.5
     1990.3
     1226.3
      625.5
      301.7
      156.7
                               155

-------
                                                          (8  of 9)
                 24 HOUR  TOTALS
DAILY PROBABILITY OF EFFLUENT CONTAINING ORGANISMS .35
OIST(MI)
    0. 10
    0. 15
    0.20
    0. 30
    0.50
    0.75
    1.
    1.
    2.
   ,00
   ,50
   ,00
    2.
    3.
    4.
   ,50
   ,00
   ,00
 5.00
 7.00
 9.00
10.00
12.00
15.00
20.00
25.00
ORG/M3/DAY
  667715.0
  121721.7
   51434.2
   22319.7
    7181.8
   43652.5
   29776.7
   31907.0
   33841.0
   49469.3
   81566.3
   53726.0
   33744.1
   21888.9
   16907.8
   14498.0
    7860.7
    6416.7
    4064.0
    3019.2
ORG/M2/DAY
 1213064.7
  181843.5
   79039.1
   32838.1
   10923.7
  105587.5
   63629.8
   54603.6
   50376.2
   63930.6
   91776.7
   51810.4
   25768.8
   11676.0
    6445.6
    5156.7
    2123.4
    1348.5
     669.5
     389.6
                              156

-------
                                                    (9 of 9)
              SUMMARY  UF  RESULTS  	
DISK MI)       AVG  NO.  PART.        PERCENI  AFFECTED
                  INGESTED/IND.           BY EFFLUENT

   0.10            1880779.7                  20.000
   0.15             303565.1                   5.182
   0.20             130473.2                   0.000
   0.30              55157.8                   1.640
   O.50              18105.4                   1.041
   0.75             149240.0                   7.946
   1.00              93406.6                   0.127
   1.50              86510.6                   1.380
   2.OO              84217.3                   0. 195
   2.50             113399.9                   1.183
   3.00             173342.9                   0.473
   4.00             105536.4                   5.531
   5.00              59513.0                   2.343
   7.00              33564.9                   1.877
   9.0O              23353.5                   1.702
  10.00              19654.7                   1.159
  12.00               9984.1                   0.172
  15.00               7765.2                   0.344
  20.00               4733.5                   O.OO1
  25.00               3408.7                   0. 789
                             157

-------
                                                              (1 of 9)
                         FIGURE 21

*###*#***#*##**CGGLINO TOWER FIXED PARAMETERS***************
TOWER HEIGHT (FEET)
TOWER DIAMETER (FEET)
HEAT LOSS (MEGACAL/SEC,MAX)
TEMPERATURE RANGE  (DEC F)
DRIFT FRACTION (G/G)
CONCENTRATION RATIO  (G/G>
EXIT VELOCITY (FT/SEC)
                      450.00
                      200.00
                     1200.00
                       2b.OO
                    0.000100
                        1. 40
                       20.00
           —COOLING TOWER OPERATING PARAMETERS—
TIME (HRS)
   0- 400
 400- 800
 800-1200
1200-1600
1600-2000
2000-2400
DRY BULB T
  55.00
  55.00
  60.00
  65.00
  63.00
  58.00
WET BULB T
  52.00
  51.00
  55.00
  50.00
  61. 00
  54.00
WIND VEL.
 20.00
 25.00
 10.00
 20.00
  5.00
 10.00
OPER CAP
 ,00
 ,00
 ,00
1.00
1. 00
1. 00
STABILITY
5.
5.
3.
5.
6.
6.
                                     158

-------
                             (2 of 9)
    COOLING TOWER AND ENVIRONMENT AL PARAMETER

PROBABILITY OF EFFLUENT CONTAINING ORGANISMS .
HEAT LOSS ( MEGAC AL / SEC )            1 200 . 00
DRY BULB TEMPERATURE ( DEG F)         55.00
WET BULB TEMPERATURE (DEG F)         52.00
WIND VELOCITY (KNOTS)                20.00
                    0-  4OOHRS
DIST















1
1
1
ji
(Ml) PLUME RISE
3
3
174.
504.
857.
3152.
v
1
319.
778.
2589.
'",•'
1






344.
395.
875.
788.
551.
441.
293.
204.
0
0
0
0
8
1
0
4
4
1
7
1
V
9
6
0
8
5
0
o
ORG/M2/4HRS
0.
0.
0.
0.
14334.
2986 .
3898.
3391.
2435.
1561.
1032.
1280.
974.
388.
192.
173.
92.
74.
36.
17.
0
0
0
0
5
6
9
3
9
1
4
4
1
3
4
•-•
9
3
2
5
159

-------
                                                             (3 of 9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS   400-  SOOHRS
PROBABILITY OF EFFLUENT CONTAINING ORGANISMS-
HEAT LOSS (MEGACAL/SEC)            1200.00
DRY BULB TEMPERATURE  (DEG f-)         55.00
WET BULB TEMPERATURE  (DEG F)         51.00
WIND VELOCITY (KNOTS)                25.00
55
DI!
T(MI )
0. 10
0. 15
0.20
0.30
0.50
0.75
1 . 00
1.50
2.00
2.50
3.00
4.00
5.00
7.00
9.00
10.00
12.OO
15.00
20.OO
25.00
PLUME RISE(M)
65.6
86.0
104.2
136.5
191.8
251.4
274.6
274.6
274.6
274.6
274.6
274.6
274 . 6
274.6
274.6
274.6
274.6
274.6
274.6
274.6
ORG/M3/4HRS
0.0
0.0
451920.5
96629.8
26797. 7
11319. 1
7428.3
24613.5
16962.5
27216.1
20955.4
14516.9
22598.8
16770.9
11887.8
10699.0
7191.8
4834.0
3207.3
2565.8
   ORG/M2/4HRS
           0.0
           0.0
      620810.7
      132741.9
       36812.4
                                                      15549.
                                                      10204.
                                                      27387.
                                                      16835.
                                                      21030.
                                                      14105.
                                                       8425.
                                                      11175.
                                                       5759.
                                                       3306.
                                                       2976.
                                                       1580.
                                                        813.
                                                        396.
             2
             4
             7
             5
             3
             5
             5
             9
             6
             9
             2.
             7
             5
             5
                                                        317.2
                              160

-------
                                                             (4 of 9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS  800-1200HRS
PROBABILITY OF EFFLUENT CONTAINING ORGANISMS-
HEAT LOSS (MEGACAL/SEC)            1200.00
DRY BULB TEMPERATURE  (DEG F)         60.00
WET BULB TEMPERATURE  (DEG F)         55.00
WIND VELOCITY  (KNOTS)                10.00
                                              ,55
DISK MI )
    0. 10
    0. 15
    0.20
    0. 30
    0.50
    0.75
    1. 00
    1. 50
    2.00
    2.50
    3.00
    4. 00
    5.00
    7.00
    9.00
   10.00
   12.00
   15.00
   20.00
   25.00
ISE(M)
164. 1
215. 1
260.5
341.4
479.9
628.8
761.8
998.2
1209.2
1382.8
1382.8
1382.8
1382.8
1382.8
1382.8
1382.8
1382.8
1382.8
1382.8
1382.8
ORG/M3/4HR8
0 . 0
0 . 0
86868.0
18574. 1
5151.0
2175.7
1427.9
2106.5
443.7
865. 1
1638.4
1 030 . 9
1539.4
1069.4
1630. 3
1672. 1
1281.0
935.6
566.3
380.5
                                                  ORG/M2/4HRS
                                                          0.0
                                                          0. 0
                                                          0.0
                                                          0. 0
                                                          0. 0
                                                          0.0
                                                          0.0
                                                       3827.0
                                                        806.2
                                                       1437.9
                                                       2250.7
                                                       1023.2
                                                       1353.4
                                                        620.7
                                                        806.3
                                                        694.8
                                                        439.9
                                                        260.3
                                                        124.5
                                                         64. 0
                               161

-------
                                                            (5 of 9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS 120O-1600HRS
PROBABILITY OF EFFLUENT CONTAINING
HEAT LOSS (MEGACAL/SEC)
DRY BULB TEMPERATURE (DEG F)
WET BULB TEMPERATURE (DEG F)
WIND VELOCITY (KNOTS)
ORGANISMS
1200.00
  65.00
  50. 00
  20. 00
DIST(MI)
0. 10
0. 15
0.20
0.30
0.50
0.75
1 . 00
1.50
2.00
2.50
3.0O
4 . 00
5.00
7.00
9.00
10.00
12.00
15.00
20.00
25.00
PLUME RISE(M)
76.9
100.7
122.0
159,9
224.7
279.0
279.0
279.0
279.0
279.0
279.0
279.0
279.0
279.0
279.0
279.0
279.0
279 . 0
279.0
279.0
ORG/M3/4HRS
126221.7
34704.2
17073.7
7061.8
2567.6
1287. 4
965.5
643.7
2950.7
5455.8
4325.4
2922.2
4919.6
3097.5
4741.9
4881.4
3751.9
2747.9
1667.2
1122.4
ORG/M2/4HRS
123919.0
34071. 1
16762.2
6933.0
2520.8
1263.9
947.9
632.0
2434.2
3719.6
2661.4
1439.4
1886.7
892.2
1163.8
1006.7
639.5
379.4
181.9
93.7
                               162

-------
                                                              (6  of  9)
    COOLING TOWER AND ENVIRONMENTAL  PARAMETERS 1600-2000HRS
PROBABILITY OF EFFLUENT CONTAINING ORGANISMS
HEAT LOSS (MEGACAL/SEC)            1200.00
DRY BULB TEMPERATURE  (DEG F)          63.00
WET BULB TEMPERATURE  (DEG F)          61.00
WIND VELOCITY  (KNOTS)                  5.00
                                            02
DIST(MI)
    0. 10
    0. 15
    0.20
    0.30
    0.50
    0.75
    1.00
    1.50
    2.00
    2.50
    3.
    4.
    D.
    7.
   ,00
   00
   OO
   00
 9.00
10.00
12.00
15.00
20.00
25.00
PLUME RISE(M)
        302.2
        321.0
        321.0
        321.0
        321.0
        321.0
        321.0
        321.0
        321.0
        321.0
        321.0
        321.0
        321.0
        321.0
        321.0
        321.0
        321.0
        321.0
        321.0
        321.0
ORG/M3/4HRS
425005. 7
116853.8
57489.4
921603.3
1649865. 0
903697.7
425025. 1
188900.0
24126.3
15440.8
8866.7
4987.5
6152.0
3071.0
2106.5
1798.6
1368.2
979.0
635.9
455.0
ORG/M2/4HRS
0 . 0
0.0
0.0
2247629.7
2414239.9
88 1 584 . 5
310968.6
92138.8
8826.0
4518.9
2162.4
912.3
900 . 2
321.0
171.2
131.6
83. 4
47.8
23.3
13.3
                                163

-------
                                                             (7  of-9)
    COOLING TOWER AND ENVIRONMENTAL PARAMETERS 2000-2400HRS
PROBABILITY OF EFFLUENT CONTAINING ORGANISMS
HEAT LOSS (MEGACAL/SEC)            1200.00
DRY BULB TEMPERATURE  (DEG F)         58.00
WET BULB TEMPERATURE  (DEG F)         54.00
WIND VELOCITY (KNOTS)                10.00
53
DIST(MI)
    0. 10
    0. 15
    0.20
    0.30
    0.50
    0.75
    1.00
    1.50
    2.00
    2.50
    3.00
    4.00
    5.00
    7.00
    9.00
   10.00
   12.00
   15.00
   20.OO
   25.00
SE(M)
1 55 . 9
204.3
247.4
26 1 . 9
261.9
261.9
261.9
261.9
261.9
261.9
261.9
261.9
261.9
26 1 . 9
261.9
26 1 . 9
261.9
261.9
261.9
261.9
ORG/M3/4HRS
878180.6
133648.3
59352.9
34801.3
196764.4
109327.0
163549.8
92746.8
135216.3
122557.0
93545.9
63924 . 4
41248.0
24755.7
17035.9
13340.6
11117. 1
1717.9
1288.4
702.8
   ORG/M2/4HRS
     1459709.3
      222149.8
       98656.2
       57846.7
    •  243944.4
      108508.1
      126377.5
       53829.8
       66869.6
       50928.5
       32126.4'
       17782.4
        9066.1
        4165.9
        21O6.2
        1145.4
         954.5
          94.4
          70.8
          21.7
                              164

-------
                                                         (8 of 9)
                        C_" T' ft T AI '""'   -.—«___ — —. — — — _„ — — — —
                         v I U I Hl_--'


DAILY PROBABILITY OF EFFLUENT CONTAINING ORGANISMS
                                 ORG/M2/DAY
                                   1583628.4
                                    256220.9
                                    736229. 1
                                   2445151.2
                                   2711851.9
                                   1009892.3
                                    452397.4
                                    181206.7
                                     98207.3
                                     83196.3
                                     54338.8
                 .37
DIST(MI)
0. 10
0. 15
0.20
0.30
0.50
0.75
1 . OO
1 . 50
2.0O
2.50
3.00
4 . OO
5.00
7.00
9.00
10.00
12.00
15.00
20 . 00
25 . OO
ORG/M3/DAY
1429408.0.
285206 . 3
672704.5
1078670.3
1891580.5
1029981.0
601900.6
312868.0
182851.9
173853.9
131110.6
8997 1 . 1
78802.0
50160.4
38278. 0
33179.6
25262.0
11655.8
7658. 1
5430.5
30863.
25356.
12147.
 7746.
 6128.
 3790.
 1669.
  833 .
                                           5
                                           7
                                           9
                                           0
                                           9
                                           5
                                       V>"7 S
                                       1 *- / . v-1
                               165

-------
                                                     (9  of  9)
              SUMMARY  OF  RESULTS
DISK MI)
AVG NO. PART.
  INGESTED/1ND.
                                  PERCENT
                                       BY
AFFECTED
EFFLUENT
 O. 10
 0. 15
 0. 20
 0. 3O
 0. 50
 0. 75
 1. 00
 1. 50
 2. 00
 2.50
 3. 00
 4. 00
 5. 00
 7.00
 9. 00
10.00
12.00
15.00
20. OO
25.00
                  3013036.4
                   541427.2
                  1408933. 6
                  3523321.4
                  46O3432.4
                  2039873.3
                  1054298.0
                   494074.6
                   281059.2
                   257050. 1
                   185449.4
                   120834.3
                   104158.4
                    62308. 1
                    46024.9
                    39307.6
                    29052.9
                    13325.3
                     8491.2
                     5958.0
                             20.OOO
                             11.^57
                              19
                              20
                               0
                               7
    958
    000
    311
    159
  4. 150
  7.704
    682
    O02
    660
    400
    561
    319
    991
    284
  1. 169
  2.601
  1 .955
  0.000
                               1
                              15
                              15
                               1
                               ••;•
                               J-,
                               6
                               1
                               3
                             166

-------
F. Program History

     Figure 22 is a listing of the simulation program.   The
program is written in Fortran IV for use with the DEC-20 system.
Data is inputted via a data file (e.g.  FOR 28. DAT) that con-
tains the input data in free format.
                             167

-------
                                                (1 of 8)
001 00
00200
003OO
00400
00 SCO
00600"
00700
00800
00900
0 1 000
0 1 1 00
0 1 200
01300
01400
0 1 500
01600
01700
0 1 800
0 1 900
02OOO
02100
02200
02300
02400
02500
02600
02700
02800
02900
03000
03 1 00
03200
03300
03400
03500
03600
03700
03800
08900
0400O
04 1 00
04 1 1 0
04120
04130
04140
04200
0430O
04400
04500
04600
04700
0480O
04810
O4W2U
i^
C
c
C
c
c
c
c
c























8

C

c











•-'





                   FIGURE 22

©TYPE (FILE ) PLUMUD„ I- OR
   PROGRAM PLUMUD  —A. 02 Zo—  10725778
   7HIS PROGRAM SIMULATES  I HE  PROBABILITY
   THE DOWNWIND DISTRIBUTION AND POSSIBLE
   A POPULATION BY POTENTIALLY INFECTIOUS
   COOLING TOWER DRIFT.   I HE DISTRIBUTION
   EFFECTS OF THESE ORGANISMS  ARE  BASED  ON
                                        OF OCCURRENCE
                                        INFECTIUN IN
                                        ORGANISMS FROM
                                        AND POTENTIAL
                                         DATA FROM
   THIS REPORT.   THE DRIFT COMPUTATIONS  ARE BASED-ON THE
   ORFAD MODEL AS DESCRIBED BY M. E.  LAVERNE IN REPORT
   ORNL/TM-520I  (OAK RIDGE NATIONAL  LABORATORY).
   DIMENSION
   DIMENSION
   DIMENSION
   DIMENSION
   DIMENSION
          TBL<6,5) vDIAM(7) , R U ) , XM (21 ) •, VI (25) , VF (25> 2)
          DF(25,2) , CLIMUL ( 25 ) ,DRFRAC( 7) ,TGI(6) , ISED(6)
          DRIF I ( 20) v DR (20 ) v 1K (20 ) , PRR (20 ) .. A ( 6) , P ( 4 ) > B ( 4 )
          TOMS(20),TGMC(20),TOTC<20), TOTS(20)
          ARY(20),T OPP(20)
DATA TG17-.0263, -. 0173v -. 0146v -.01 •, . OU46-, . 02637
DATA A/-7.9,5.03,-1.38E-7,11.3,8.13E-3,-3.497
DATA B7-9.1,-3.57,.88>.0067
DATA DIAM/7O.,175.,300.,125.,550.,700.,0.7
DATA DRF RAC/ . 06, . 3, . 39 •. . 15 •, . 07 •> . 03 > 6. /
DATA G,PI,COBRT>FTM79.81,3.1416,.33333,.3O487
DATA XM7. 1 v . 15> .2. .3> .5, .75, 1. > 1.5v2,2.5v3. ,
14. > 5. , 7. ,9. , 10. i 12. •, 15. ,20. ,25. ,0. 7
AB(Y)-255.37+.5555*Y
OPEN(UN1T-7,ACCESS^'SEUOU T • ,FILE~"'PLUM.DAT' )
LR=26

PROBA-0.
DO 8 I~l,20
TOTS(I)=0.
fOTC(I)--0.
CONTINUE

READ INPUT DATA AND  WRITE FIXED DATA
READ(LR•*)HIO,LIAT 0,F RACDR,EXSPDO,CONC,TMPR,HEA TO
WRITE FIXED PARAMETERS
WRITE(7,1000)
WRITE(7-1003)HTO
WRITE(7,1005)DIATO
WRITE(7,1007)HEATO
WRITE(7,1030)TMPR
WR IT E ( 7 •, 1031 ) F RACDR
WRI TEC 7.
WRITE(7,
WRITE ( 7.
DO 5 I~l
READ ( LR , * ) ( TBL ( 1, J) , J~.". , •.
CONTINUE
READ ( LR ,•«•)( ISED ( I ) , 1 ~ 1 , 6.
LTIMt=0
NlIME-400
WRITE(7,1034)
WR1TE(7,1035)         _„_
                      loo
           1032)CONC
           1033)E XSPDO
           1012)
            6

-------
                                              (2  of  8)
MTIME-0
KTIME-400
LIU 3 UK" 1 , 6
WRITE. ( .•'•, 1036)1*11 IME,KT1ME
MTIME-MT1ME+400
K7IME-K71ME+400
CONTINUE
DO 80U NCY" 1 , 6
(7BLUIK-, .JL> , JL-1 >5
05000
05 1 00
05200
05300
05400
05500
05600
05700
o?sft("M") f:
05900
06000
06 1 00
06200
0630O
06400
0650O
06600
0 7 1 00
07200
07300
07400
07500 C
07600
07700
078OO
0790O
O:-:OOO f:
03100
OS200
OS300
08400
08500 1 1
08600 1 3
03700 15
08800 1 7
08900 1 9
OvOOO 21
09100 23
09200 25
09300 27
09 4 00 10
09500 12
096 OO 14
097OO 16
DBr=TBL * 1 VOL / LIM W
ATAU-TAU/2
BINVOL"UMW*AI"AU

CALCULATE PROB. OF ORO PRE8EN I
c-o.
DO 30 I-1-, 2000
Y-OOUBF( I3ELK 1 ) )
IF ( Y-.064) 10, 1 1
IF ( Y-. 1380) 12, 13
IF (Y-. 2686) 14, 15
IF (Y--. 31 58) 16,17
IF (Y-.4444) 18, 19
I F
-------
                                            (3 of 8)
09800
O99OO
1 0000
10100
1 0200
10300
10400
1 0500
1 0600
107OO
10800
1 0900
1 1000
11100
11200
1 1 300
11400
1 1 500
1160O
1 1 700
11800
1 1 900
1 2000
1 2 1 00
12200
12300
12400
1 2900
1 3000
13100
1 3200
1 3300
13400
1 3500
1 3600
13700
1 38i JO
1 3900
14000
1 4 1 00
14200
1 4300
14400
14500
14600
14700
14800
14900
1 5000
1 5 1 00
15200
18
20
22
24
26
28
29
40
30




^'

C







200
205
2 1 0

























   2~.4;GO  TO  40
   Z=.5'GO  TO  40
   Z-.65GG  TO  40
   Z=.8;GG  TO  40
   Z~.8;GO  TO  40
   Z-.95GO  TO  40
   Z~l.
   L>C+Z
   CGNIINUfc
   PROB=C/2000.
   XY=AB8(GGNOF(1SED(3)))
   IFCXY.Gl.1.)GO TO 2
   PROB=XY*PRGB
   PROBA=PROBA+PROB

   CALCULATE  INPUT PARAMETERS
   CTORG-0.
   NTRY-Y*20
   DO 210 1 = 1 , NTRY
   CALL GGEXPt 18tD(2) vPKOBv 1 , R<1 ) )
   CNGRG=R< 1 )*1.E5
   RV2-OGUBF( 1 SEEK 3) )
   1 F ( RV2-PROB ) 200 , 205
   CNGRG-0.
   C TNORG-CNURG+CTNGRG
   CONTINUE
SEE ORFAD MODEL.
   DIAMAX=D1 AM( IDRCL)*! . 5-. 5*DIAM< IDRCL-1 )
   D I AM ( 1 DRCL + 1 > ~L> I AM A X
   D=0 .
   DD=DIAMAX/25.
   F"'0.
   xi-o.
   X2=.5*(DIAM< 1 )+LHAM<2) )
. 7*CONR
   CGNR=.01
   FAC=1 . E-4 «•((!
   DO 240 -J-l ,25
   D--D+DD
   DIM-FAC*D
   VFJ2-3519.2*D1MH-D1'M
   VFJl-.4963-=WF..J2
31 12)«-*CUBRT
   IF(D.GT.74.36)Vl.J~.00445-»-(D-37. 18)
   DF.J 1 -DF.J-* ( ( V .1 .J-VFJ1 ) / (V1J+VFJ1 ) )
   DFJ2-DF J* ( VIJ -VF.J2) / ( VI ..1+VF J2)
   VF(.J, D-VFJ1
   VF( J»2)-VF.J2
   VI(.J)=V1J
   DF(.J, 1 )~DFJ1
                         170

-------
                                                             (4  of  8)
1 5300
1 5 4 CO
1 5500
1 5600
15700
1 6200
1 6300
16400
16500
1 6600
16700
1 6800
1 6900
1 7000
17100
17200
1:7300
1 7400
1 7500
1 7600
1 7700
17800
1 79OO
1 8OOO
18100
1 8200
1 8300
13400
1 8500
1 8600
1 8700

215
220

230
2 3 2

235


240

250

C





1 30

1 1 0








                 lF(D-X2)220,220v 230
                 CUMUL ( J > "F+DRFRAC < K ) * ( D--X 1 ) / ( X2~X 1 )
                 GO TO 240
                 K-IDRCL
                 F-FrDRFRAC(IDRCL)
                 X2--DIAMAX
                 IF(J.LT.25)00 TO 215
                 CUMUL(J)-1.
                 CONTINUE
                 DO 250 J= 1 •, 24
                 CUMUL (26-J) -CUMUL (26-J) -CUMUL (25-.J;

                 CALCULATE RELATIVE HUMIDITY
                 TEO=AB 140
PVSD=PVSF#2. 063
GO TO 1 30
PVSW-PVSF-s-2.036
                                 *( l.+(WBT-32
                       •«• 1 . 07 9
HH^PV/PVSD
IF(RH.LE.O. )I-
8'HFAC- ( 1 . --RH
l'RH-2
IF(RH.L'I . 0.5
CALCULATE TOWER  EFFLUENT  TEMPERATURE
IF I WBT . L I . 80>ENI AL-~( WBT-+-4. 31 ) / ( 7. 92-. 0248-*WBT)
                                       171

-------
                                                       (5 of 8)
21100
21200
2 1 300
21400
21500
21600
21700
21800
21900
22000
22100
2STAB)
22600
22700
22800
22900
23000
23100
23200
23300
23400
23500
23600
23700
23800
23900
24000
24100
2420O
24300
24400
24500
24600
24700
24800
24900
25600
25700
25800
259OO
26000
26100
26200
310
320



330

340



 C
                 I F < WBT . GE . SO . ) ENT AL~ ( WBT- 1 3 . 85 ) / ( 2 . 77- . 0 1 6*WB7 )
                 RAPP=TMPR*APP
                 ENT AL-ENTAL+RAPP
                 IF(£NTAL.GT.43.7> T3~(2.766#ENIAL+13.85>/< 1 . +. 016*EN TAD
                 IF(ENTAL.LE. 43. 7H3~( 7. 92*ENiTAL-4. 31 ) / ( 1 . +. 0248*ENTAD
                 T'PO-AB(TS)
                 DELQ1-EVLQS/(753130*EXVUL>
                 DELQ=DELQ1*TPO
                 UNWIND
                 FC-0.
                 DO 361  IX-1,20

                 TEHT-TEQ+7G*HT
                 S-G* ( TG+ . 0 1 ) / TEO
                 CONTINUE
                 FR=G#EXSPD*RAD*RAD*(1 . -TEHT/TPO+DELQ*< . 61+2545. *FC/TPG) )
                 IF(FR.GE.O. ) GO TO  310
                 TEHT-TPO
                 GO TO 300
                 CONTINUE
                 IF25
                 OR-O. '
                 OPR=0 .
                 OXME^O.
                 l'NCR-1
                 DO460  IX-1,20
                 DRIFT(IX)-0.
26300
26400
26500
26600
26700
26800
26900
27000
C
                    XM( IX)*1609
                PR-PRR(I X)
                DPR^PR-OPR

                CALCULATE DRIFT
                DXME-XME-OXME
                OPR-PR
                CiXME=XME
                                      172

-------
                  (6 of  8)
27 1 00
27200
27300 C
27400
27500
27600
2/700
27800
27900
28000
28100
28200
2830O
28400
28500
2860O C
28700 410
28800
28900 420
29OOO
29100
29700
29800
29900 430
30000
30100
30200
303OO
30400 440
30500
30600
30700 491
3O8OO 46O
30900
3 1 000
3 1 1 00
3120O
3 1 30O
31400
31500
31600
31700
31800
3 1 900
32000
32 1 OO
32200 470
82300
32400
82500
32600
32700
32800
:'-::•• 9 C')O
IF (PH. t "I". <">. 7A) HII in 4j.o

NO EVAPORATION
VFALL- ( HT+PR ) #U7 XME
DIA-37. 13+Vi 'ALL/. 00 '1 -'15
IF C VFALL. LI .0. 1655; D i A~S'QRT ( 734 1 4 . •* VFALL)
IDIA-DIA/DD+1 .
IK( IX)-aDIA
I F ( 1 D I A . 1'iT . 25 ) MM T r i 4'-' l
DVDR^ ( VFALL-U*DPR7DXME ) / XME
DDDR^-DVDR/ . 0045
I F ( VFALL . L T .0.1 665 ) DDDR- . 5*D I A#DVDR7 VFALL
DCLiD-CUMUL ( I D 1 A ) /DO
GO TO 44 O

EVAPORATION
CONTINUE
H--HI+FR
RY^DF < K .. 1 RH ) 7RFIFAO
IF(H.LT.RY) GO TO 450
XR-ll-H- ( H--R Y ) 29600 K~K~- 1
OR=XR
IF (K) 491, 491 ,42O
DCDD~CUMUL < K )
IK( IX)~K
IF GO TO 491
VFALL-VF(K, 1RH)
DDDR-1. /(XR-OR)
CHI2^ARLOS^-DDLiR-«-DCDD«-lNCR«-8. / ( XME^F'I >
DR( IX)-CHI2/VFALL
DRIFT ( 1X)=CHI2
CUNTINUE
CONTINUE
IF
1 F ( RAN V . L I" .U.I ) (".MOD:- . 5
IF "T OT 8 ( I Y ) + TOMS' <. i Y )
CONTINUE
WRITE (7, 1D12)
WR I T E (7,1 00 1 ) L T 1 ME , N 1 1 ME
URITE ('/••, 1002) PROM
WR1 IE( 7 , :l.006)HEA!0
WRITE (7-, 1008HJBI
WR.LTET7, 1009)kit.
-------
                                                           (7 of 8)
33000
WRITE(7,1011)
33100
33200
33300
33400
33500
33600
33700
33SOO
34 1 00
34200
34300
34400
34500
34600
34700
34800
34900
35000
35100
35200
35300
35400
35500
35600
35700
35300
35900
36000
36 1 00
36 1 1 0
36200
3A3OO
36400
36500
36600
36700
3 6 800
36900
37000
37100
37200
37300
37400
375OO
37600
37700
37800
87900
380OO
38100


600


800

1 00 1
1 003
1 005
1 006
1 007
1 008
1 009
1010
1011
1 OOO

1 0 1 5





700
1012
1016

1018

1017
1 0 1 9
1020










650



750


                                     PRR(IR),T OMCd R ) » TOMS(IR)
WRITE(7,1016)
DO 600  IR-1,20
WRITE (7, 1015)XMUR)
LTIME=LriME+400
NTIME--LTIME+400
CONTINUE
PROBB=PROBA/6
FORMA T ( - 1 - , •-     COOL I NMS -' , F 4. 2)
F ORMAT(IX,- TOWER HE IGHT  (F EEI )
            TOWER DIAMETER (FEET)
            HEAT  LOSS  (MEGACAL/SEC)
            HEAT  LOSS  (MEGACAL/SEC,MAX)
            DRY BULB  TEMPERATURE (DEG F)
            WET BULB  TEMPERATURE (DEG F)
            WIND  VELOCITY (KNOTS)
FORMAT (IX,
FORMATdX ,
FORMAT (IX,
FORMAT ( IX >
FORMAT ( IX,
FORMAT ( IX -i
FORMAT <•*•*• -if- *•*•#•«••«••«••?<• *•*••«• #COOL 1 NG T OWER F I XED PAR
FORMAT (2XvF7.2, 1 OX •, F8. 1 •, 5X •, F 12. 1 •, 5X , F12. 1 )
WRITE(7,1012)
WRITE(7-, 1018)
WRITE(7,1020)PROBB
WRITE(7,1019)
DO 70O  IM-1,2O
WRITE(7, 1O17)XM( IM) , TOFC( IM) •, TOTS( IM)
FORMAT(////)
FORMAT (1 X , •"DIST ( Ml ) "' , 5
1 , 6X, -'ORG/M2/4HRS-' )
FORMAT ( -' 1 - , /// ,  	
                                          PLUME RISE(M) "'v6X> •"ORG/M3/4HRS
                                                    24 HOUR TOTAL
                                                                 '•.'  — ™-
                 FORMAT (2X,F7.2S5'X3F 12. 1 v5X>F12. 1 )
                 FORMATdX, •'DISTtMI) •"•, 7X, - ORG/M3/DAY •' , 7X, •'ORG7M2/DAY-' )
                 FORMAT ( IX, -DAILY PROBABILITY OF  EFFLUENT  CONTAINING  ORGA
                 1NISMS-' , F4.2, / )
                 DO  7'50 J~l > 20
                 TOPP ( J ) -20 . *"l ' 0 1C ( ,J ) H-TO 1" 3 ( J )
                 PL-O.
                 PK'-O.
                 DO  650 M- 1 7 1 00
                 CALL GGEXP ( I SED ( 5 ) , . 1 , 1 , R (1 ) )
                 DORG-3. E6-K-R( 1 )
                 I F ( TOPP ( J ) . G T . DORG ) PK~PK+ 1 .
                 FL=PL+1.
                 CONT 1NUE
                 AND-ABS < GGNOF ( I SED ( 6 ) ) )
                 ARY ( J ) -AND*PK#20. /PL
                 I F ( ARY ( J ) . G T .20. ) ARY ( J ) ^20 .
                 CONTINUE
                 WRITE •: /•, 1025)
                 WRITE< 7 > 1026)          174

-------
                                                             (8  of 8)
38 1 00
33200
38300
38400
33500
38.600
387OO
jyyoo
38900
39000
39100
39 1 1 0
39 1 20
39




770
1 025

1 026

1 028
1 027
1 030
1031
F10.2)
                 WR1TE(7,1026)
                 WRI IE ( 7 >1028)
                 DO 770 N"1,20
                 WR I IE (7,1027 ) XM < N) , T GPP ( N ) , ARY ( N )
                 CONTINUE
                 FORMAT C' 1 •",///•< •"	    SUMMARY Oh RESULTS

                 FORMAT ( 1 X , // , 2X > ''D1 S"f ( M1 ) '' , 7X •, '' AVG NO.  PAR I . '' , 7X , •'PERCEN
                 IT AFFECTED")
                 FORMAT (19X> ' INGESTED/ IND. '' •, 10X, "'BY EFFLUENT''' , / )
                 FORMAT(2X)F'7.2,8X,F12. 1 » 16X,F7.3;
                 FORMAT (IX,- TEMPERATURE RANGE  ( DEG  F- )        •', F 10. 2)
                 F ORMA T <1X,-DR1F T FRAC f1ON  (G/G)             " ,F10.6)
39150   1034     FORMA'K 12X, -—COOLING TOWER  OPERATING PARAMETERS—'')
39160   1035     FORMAT(1X>"!1ME (HRS ) ' 4X , - DRY  BULB T" , 4X , - WE T BULB  T''
39170            1,-'WIND VEL.'',4X,-OPER CAP'" » 4X,''STABILITY''>
39180   1036     FORMAT ( IX, 14, ''-'', 14 , 2X , F1O. 2, 4X , F10. 2, 4X , F9. 2, 7X , F4. 2
39181            16X,F4.0)
39200            END
                                       175

-------
                           REFERENCES


 1.  Adams, Paul.  1978 personal communication,

 2.  Akers, T.G0  1972.  Personal communication.  Naval  Bio-
        medical Research Lab.  Oakland, California,.

 3.  Burleson, G.R.  et_.a_l_.  1975. "Inactivation of Viruses and
        Bacteria by Ozone." Journal of Applied Microbiology.
        29(3): 340-344.

 4.  Chen, Norbert, CoJ. and Steven R. Hanna.  Drift-Modellag
        and Monitoring Comparisons.  Presented at the  Cooling
        Tower Institute meeting, 1977.  (CTI/TP 175A)

 5.  Cummings, R.O.  1964.  The Use of Municipal Sewage  Effluent
        in Cooling Towers.  Presented at the meeting of  the
        Cooling Tower Institute, June.

 6.  DeJong, J«C.  et_.aJL  1974.  Inactivation of Encephalo-
        carditis Virus in Aerosols.  Journal of Applied  Micro-
        biology, 27(1): 57-65.

 7.  Dimmick, RaL»  1965.  Rhythmic Response of Serratia Mar-
        cescens to Elevated Temperatures„   Journal of  Bacter-
        iology.  89:791, 798, 1965.

 8.  Dvorn, R. and R. Wilcox0  1972.  Treated Sewage for Power
        Plant Make-up Water.  Power Engineering, November, pp. 40-41

 9.  Elliot, Thomas C0  "Cooling Towers: Special Report," Power.
        March 1973.

10.  Erlich, R. and S. Miller.  1968.  Survival of Pasteurella
        Tularensis at Different Atmospheric Temperatures.  IIT.
        Report BLTM 143.Fort Detrick, Maryland, December 1968.

11.  G-oetz, A.  1954.  Early Detection of Bacteria Growth.  In:
        Proceedings of the Atmospheric Biology Conference.
        University of Minnesota.

12.  Goldberg, L.J.  1977.  Personal communication.

13.  Harper, G.J.  1961. "Airborne Microorganisms Survival Test
        With Four Viruses." Journal of Hygiene.  59: 479-486.
                                176

-------
14.  Hickey, J.L.S.  and P0C0 Reist.  1975.  "Health Significance
        of Airborne Microorganisms from Wastewater Treatment
        Processes.  Part I: Summary of investigations.  Part  II:
        Health Significance and Alternatives for Action." Journal
        of Water Pollution Control Federation.  47(12): 2741-2733.

15.  Jensen, M.M0  1964. "Inactivation of Airborne Viruses by
        VCV. Irradiation." Journal of Applied Microbiology.
        12(5):  418-420.

16.  Jolley, Robert.  1978.  Personal communication.

17.  Leffler, E.  and Y. Kott.  1974.  Virus Retention and
        Survival in Sand,  In: Proceedings for Center For
        Research in water Resources Symposium #7.  84-91.
        University of Texas, Austin, Texas.

18.  Leif, W.R0  and J0E. Hebert.   1977.  Personal communication.
        Naval Biological Laboratory, Oakland, California.

19.  Lewis, Barbara Ann G0  1978»   Personal communication.

20.  Levine, H.B0  1977.  Personal communication.  Naval Bio-
        logical Laboratory.

21.  Lewis, BoGo  1974. "On the Question of Airborne Transmission
        of Pathogenic Organisms in Cooling Tower Drift." In:
        Proceedings of the annual meeting of the Cooling Tower
        Institute.

22.  Lighthart, Bruce.  1972. "Survival of Airborne Bacteria  in
        a High Urban Concentration of Carbon Monoxide." Journal
        of Applied Microbology.  25(8).

23.  NUS Corporation.  1974. "Potential Virus Hazards from
        Cooling Towers Operated with Polluted Surface Water.
        Public Service Electric and Gas Company, Newark.

24.  Seidler, R.J.,  et^a_l_.  1975.  "Potential pathogens in the
        environment: K.  Pneumoniae.  Applied Microbiology.
        29(6):  819-825, June 1975.

25.  Tigertt, W.D.  §t_.a_l.  1961.  "Airborne Q Fever." Bacteriology
     Review 25:285-293.

26.  U.S. Army.  1968.  Aerosolization of Pastuerella Pestis.
        BLTM 140.  Fort Detrick, Maryland, August 1968.

27.  U.S. Army,  1965.  The Effect of Mustard on Escherichia
        Coli/Unbalanced Growth. E.A.  CRDLR 3257.  Edgewood
        Arsenal,Edgewood,New Jersey.

                                177

-------
28.  U.S. Array.   1969.  Effect of Temperature  on  Serratia-
        Marcescens.   Illinois Institute of Technology,  Chicago,
        April  1969.

29.  U.S. Army.   1966.  Estimate of  Line  Source.   APGTR66-50.
        Aberdeen  Proving Grounds, Maryland, August 1966.

30.  U.S. Army.   1953.  Field Evaluation  of Dried Bacillus  Geokii
        Spores.   DPGR 351.  Dugway Proving Grounds,  Utah.

31.  UoS. Army.   1964,,  Influence of Relative  Humidity  on the
        Survival  of P. Tularensis.   FDBLTM 50.  Fort Detrick,
        Maryland, May 1964.

32.  UoS, Army.   Long Distance Travel of  Rust  Spores.   Special
        Report 219 II and Supplement,  Camp Detrick,  Maryland.

33.  U.S0 Army.   1965.  Osmotic Effects on the Survivability of
        Pasteurella Pest is.  BLTM 69.  Fort Detrick,  Maryland.

34.  U.S. Army.   1974.  The Question Concerning the  Inactivation
        of Viruses in Air.  FTD WPAFB MTD 24 1686.   Wright  Patter-
        son Air Force Base, Ohio.

35.  U.S. Army.   1967.  Rheological Responses  of  Dry Serratia
        Marcescens to Environment Changes.  BLTM  124.   Fort
        Detrick,  Maryland.

36.  U.S. Army.   1964.  Serratia Marcescens Traced by Sarcina
        Lutea.  SES 206.  Suffreed Experimental Station, Canada,
        July 15,  1964.

37.  U.S. Army.   1965.  The Stability of  Toxic  Proteins.  Illinois
        Institute of  Technology.  I.I.T.L.  6013-15.

38.  Wellings, F0M.   et_-§L-L-  1975. "Demonstration of  Viruses in
        Ground Water." Journal of Applied Microbiology.  29(6):
        751.

39.  Wistrom, G0K.  and JoC» Ovard.  Cooling Tower Drift, Its
        Measurement,   Control, and Environmental Effects.  Ecodyne.
        Presented at  the Cooling Tower Institute  annual meeting.
        1973o  (CTI TP 107A)

40.  Zobell,, C0E.  1946.  Marine Microbiology  Chronica  Botanica.
        Waltham, Mass.

41.  U0S. Army.   1965.  Venezuelan Equine Excephalitis  Studies.
        Fort Detrick, Maryland.   BLTMS 267.
                                 178

-------
42.  Walka, R. 19760  The Distribution of Enteric Bacterial
       Aerosols Emitted From a Small Extended Aeration Activated
       Sludge Sewage Treatment Plant0   Masters thesis.  CW Post
       College, New York.

43.  Watkins,  H0M0S0 et. al.  1965.  Behavior of Colorado
       Tick Fever, Vesicular Stomatitus,  Neurovaccinia and
       Encephalomycarditis Viruses in the Airborne State,  In:
       Symposium on aerobiology (R0L0  Dimmick,ed. )pp. 381-388.
       Naval Biological Lab, Oakland,  California.
                             179

-------
                          BIBLIOGRAPHY
Abeles, F.B0   1973.  Ethylene in Plant Biology.,  Academic Press,
     New York, 302 pp.

Adams, Paul.   1978 Personal Communication.

Adlam, C. et al.  1976.  "Natural and Experimental Staphylococcal
     Mastitis  in Rabbits."  Journal of Comprehensive Pathology.
     86(4):  581-593.

Agrios.  1969.  Plant Pathology.  Academic Press, New York.

Akers, T.G.  1972.  Personal Communication.  Naval Biomedical
     Research  Lab.  Oakland, California.

Antonovics, J.  et al.  1971.  "Heavy Metal Tolerance in Plants."
     Advanced  Ecological Research.  7:  1-85.

Anver, M.R.  et al.  1976.  Dermatophilosis in the Lizard (Calotes
     Mystaceus).

Applied Science Associates, ed.  Diagnostic Vegetation  Injury
     Caused by Air Pollution,  EPA Contract 68-02-1344.  U0S0E.P.A
     Washington.

Archer, M.C.   et al.  1971.  "Environmental Nitroso Compounds:
     Reaction  of Nitrite with Creatine and Creatinine."  Science.
     174: 1341-1343.

Arnall, L.  and Keymer, I0F.  1975.  Bird Diseases Introduction to
     the Study of Birds in Health and Disease,  T0F.H. Publi-
     cations Inc.

Ashton, W.M.   1972.  "Nickel Pollution."  Nature.  237: 46-47.

Aulerich, R.J.  et al.  1972.  "Rate of Accumulation of Chlor-
     inated Hydrocarbon Pesticide Residues in Adipose Tissue of
     Mink."  Canadian Journal of Zoology.   50(9): 1167-1173.

Avilova,  G.G.   et al.  1974.  "Investigation of the Effect of
     Benzene on Adult and Young Animals."  Gig. Sanit.  6: 15-
     18.   (Abs.)

                               180

-------
Bahcock, KUM0 and Flickinger, E.L.  1977,  "Dieldrin Mortality
     of Lesser Snow Geese in Missouri,"  J.VLM,  41(1):  100-103,,

Barrett, M.W.,  and Karstad, L.H.  1971. "A Flourescent Eryth-
     rocyte Test for Lead Poisoning in Waterfowl."  J.W0Ma
     35(1): 109-119.

Baskett, TUSU  1975.  "Mercury Residues in Breast Muscle  of Wild
     Ducks."  Pesticide Monitor Journal.  9(2): 67-78.

Beardmore, C,J« and Robel, R.J.   1976.  "Weight and tiodv  Fat
     Recovery by Dieldrin-dosed Underweight Bobwhites.'   J. V.r'.H'.
     40(1): 111-117.

Becker, C.D. and T.O. Thatcher,   1973.  Toxicity of Power Plant
     Chemicals to Aquatic Life.   Battelle Pacific Northwest
     Laboratories, Richland, Washington.

Beil, H.B. and Dimmick, RVW.  1975. "Hazards to Predators Feeding
     on Praire Voles Dilled with  Zinc Phosphide."   J„W.M. 39(4):
     816-819.

Benson, N.RU  1953.  "Effect of Season, Phosphate and  Acidity
     on Plant Growth in Arsenic-Toxic Soils."  Soil  Science.
     76: 215-224.

Berger, K.C.,  1962,  Micronutrient Deficiencies  in  the U.S.
     Journal of Agricultural Food Chemistry.   10: 178-181.

Biester and Schwartz.  1965.  Poultry Diseases.   Iowa  State
     University Press.

Billings, C.E., and W.R. Matson,   1972, "Mercury  Emissions from
     Coal Combustion."  Science,  176: 3232-1233.

Biswas, N,MU  et al.  1976.  "Effect of Cadmium  on  Spermatogenesis
     in Toad (Bufo melano stictus),"  Eudokrinologie.  68(3):
     349-352.   (Abs.)

Bitman, J.  1970,  "DDT-induced  Inhibition  of  Avian Shell Gland
     Catbonic Anhydrase:  A Mechanism  for Thin Eggsliells."
     Science.   168:  594-596.

Blood and Henderson.  Veterinary  Medicine.

Bins, L.J.  et  al.   1971.   "Eggshell Thinning  in the Brown Pelican:
     Implication 01  DDE."   Diosclence.   21(24):  1213-1215.

Blus, L.J.,  1972.  "Logarithmic  Relationship of  DDE Residues to
     Eggshell Thinning."  Nature.  235:  376-377.

Borg, K.  et al.   1970.   "Experimental  Secondary Methyl Mercury
     Poisoning  in  the Goshawk  (Accipiter  gent.il is gen tills L. )"
     Environmental Pollution.   1(2):  91-104.


                                181

-------
Botzler, R0G,  et al.   1973.  "Listeria in Aquatic Animals."
     J.W.D.,  9: 163-170.

Botzler, R0G0  et al,   1975,  "Rate of Listeria Monocytogenes
     Shedding from Frogs."  J0W0D,  11(2): 2771279~.

Botzler, R.G,  et al,   1976.  "Yersiniae in Pond Water and  Snails
     JoWpD.  12(4): 492-496.

Boyce Thompson Institute.   1974,  "Effect of Aerosol Drift  Pro-
     duced by a Cooling Tower at the Indian Point Generating
     Station on Native  and  Cultivated Flora in the Area,,"   Con-
     solidated Edison Co.,  New York.

Buchauer, M0J0  1973.   "Contamination of Soil and Vegetation
     Near a Zinc Smelter by Zinc, Cadmium Copper, and Lead."
     Environmental Science  and Technology..  7: 131-134.

Braham, H0W<,  1973.  "Lead  in the California Sea Lion  (Zalophus
     Californicus)."  Environmental Pollution.  5(4): 253-258.

Braun, C.E,  et al.  1977.  "Mercury Residues in Colorado Band-
     Tailed Pigeons."   J.W.M.  41(1): 131-134.

Brenchley, W0E,  1938.  "Comparative Effects of Cobolt, Nickel
     and Copper on Plant Growth."  Annual Applied Biology.
     25: 671-694.

Briggs, G,A0  1975.  Plume  Rise Predictions.  In: Lectures  on
     Air Pollution and Environmental Impact, Chapter 3.  American
     Meterological Society.

Brooks, GoT.  1974,  Chlorinated Insecticides; Vol. II.  Bio-
     logical and Chemical Aspects,  CRC Press, Cleveland.

Broyer, T0C,  et al.  1972.  "Selenium and Nutrition of Astra-
     galus I_; Effects of Selenite or Selenate Supply on Growth
     and Selenium Content„"  Plant and Soil,  36: 635-699,

Bruner and Gcllespie.   1966.  Hagan's Infectious Diseases of
     Domestic Animals.  New York, Cornell University Press,

Burleson, G0R°  et al.  1975,  Inactivation of Viruses and  Bac-
     teria bv Ozone,  Journal of Applied Microbiology.  29(3):
     340-344,                                       ""

Busbee, E0L.  1977.  "The Effects of Dieldrin on the Behavior of
     Young Loggerhead Shrikes,"  Auk,  94(1): 28-35.

Byczkowski, J.Z,  1976,  "The Mode of Action on p,p'-DDT on
     Mammalian Mitochondria."  Toxicology.  6(3): 309-314.

                                182

-------
Cagen, S.Z. and Gibson, J0E.  1977.  "Effect of Carbon Tetrachlor-
     ide on Hepatic Transport of Ovabain in Developing Rats,"
     Proceedings of the Society of Experimental Medicine.  154(2)
     188-191.   (Abs.)'
Carter, F0L.  et al.  1971.  "l-Hydroxy-2,3-Epovychlordene in
                 Previously Treated with Technical Heptachlor."
                 Environmental Contamination Toxicology.  6; 249,
Oregon Soil
Bulletin of
Carter, P.B0  1975.  "Involvement of the Upper Respiratory Tract
     in Orally Induced SaImonellosis in Mice,"  Journal of Infec-
     tious Disease.  131(5): 570-574.

Chapman, N.D0  ed»  1966.  Zinc.  In: Diagnostic Criteria for
     Plants and Soils^   University of California, Division of
     Agricultural Sciences.  pp» 484-499.

Choquette, L.P.E,,  and Kukt, E.  1974.  "Serological  Indication
     of Canine Distemper and of Infectious Canine Hepatitis in
     Wolves (Canis lupus L.) in Northern Canada."  J,W.D.  10(4):
     321-324.,

Christensen, Herbert E., and Thomas T. Luginbyhi, eds.  19740
     The Toxic Substances List.  1974 ed.  U0S0 Department of
     HEW, Rockville, Maryland.

Clark,  D.R., Jr „  and Lament, T0GU  1976,,  "Organochloride resi-
     dues and Reproduction in the Big Brown Bat."
     249-254.
                                              J.W.M,  40(2)
Clark, D.Ro, Jr„ et al.  1975. "Organochlorine  Insecticide Resi-
     dues in the Free-tailed Bat  (Tadarida brasiliensis) at
     Bracken Cave, Texas."  Journal of Mammals.  56(2):429-443.

Clark, D.Ro, Jr. and R.M. Prouty.  1976.  "Organochlorine Resi-
     dues in Three Bat Species from Pour Localities  in  Maryland
     and West Virginia, 1973."  Pesticide Monitor Journal,   10(2)
     44-53o
Coetzer, J.A.W.,  et al.  1976.  "Cryptococcosis  in a Dog."
     Journal of South African Veterinary Association.   47(1)
     52.   (AbsTl
                                                         49-
Coleman, T.J.  et al.  1974.  "The Development of Diabetes  Cox-
     ackie B Virus  Infection in Mice."  Diabetologia  10(6):  755-
     759.  (Abs.)

Collins, F.W.  et al.  1976.  "Physiological and Biochemical
     Aspects of Cadmium Toxicity in Soybean.  Part  II.    Toxi-
     city Bio-accumulation and Sub-cellular Fractionation of
     Cadmium in Soybean Plants Grown at JSubchronic  to Acute Cad-
     mium Levels."  pp. 145-165.  tn: Trace Substances in  Environ-
     mental Health  - X., D.D. Hemphill, ed.  University of  Mis-
      souri,  Columbia.
                                 183

-------
Collins, H,L«  et al.  1974. "Residue Accumulation in Selected
     Vertebrates Following a Single Aerial Application of Mirex
     Bait, Louisiana 1971-1972."  Pesticides Monitor Journal.
     8(2): 125-130.

Cooke, A0S.  1970.  "The Effect of pp'-DDT on Tadpoles of the
     Common Frog  (Rana temporaria)."  Environmental Pollution.
     1(1): 57-72.

Cooke, A.S.  1972.  "The Effects of DDT, Dieldrin and 2.4-D on
     Amphibian Spawn and Tadpoles."  Environmental Pollution.
     3(1): 51-68.

Cooke, A.S.  1973.  "The Effects of DDT When Used as a Mosquito
     Larvicide, on Tadpoles of the Frog (Rana temporaria)."
     Environmental Pollution.  5(4): 259-273.

Cooke, A.So  1973.  "Shell Thinning in Avian Eggs by Environ-
     mental Pollution."  Environmental Pollution.  4(2): 85-148.

Crawford, G.J., Jr.  et al.  1971.  "The Frequency of Salmonella
     and Arizona Microorganisms in Zoo Turtles."  J.W.D.  7(2):
     130-132.

Crocker, A.D.  et al.  1974.  "The Effect of a Crude Oil on
     Intestinal Adsorption in Ducklings (Anas platyrhynchos)."
     Environmental Pollution.  7(3): 165-178.

Cromartie, E« et al.  1975.  "Residues of Organochlorine Pest-
     icides and PCB and Autopsy Data for Bald Eagles, 1971-1972."
     Pesticides Monitor Journal.  9(1): 11-14.

Crooke, W.M.  and R^H.E* Inkson.  1955.  "The Relationship Be-
     tween Nickel Toxicity and Major Nutrient Supply."  Plant
     and Soil.  6: 1-5.

Cummings, RoCL  1964 „  "The Use of Municipal Seivage Effluent in
     Cooling Towers "  Presented at the Meeting of the Cooling
     Tower Institute, June.

Currier, H.B0  1951.  "Herbicidal Properties of Benzene and
     Certain Methyl Derivatives."  Hilgardia.  20: 383-406.

Currier, H.B0  and S.A. Peoples.  1954.  "Phytotoxicity of Hydro-
     carbons."  Hilgardia.  23: 155-173.

Curtis, C0Ro  et al.  "Possible Effects of Salt Drift on Annual,
     Perennial, and Ornamental Species of Plants," Chalk Point
     Cooling Tower Study.  Water Resources Research Center,
     University of Maryland.  pp. 32-42.

Curtis, S.D.  and R.M.   Silverstein.  1972.  "Corrosion and
     fouling Control of Cooling Waters."  In:  Cooling Towers.
     American  Institute  of Chemical Engineers, New York.
                                184

-------
Dahlgren, RCB.  and Linder, R0L0  1974.  "Effects of Dieldrin  in
     Penned Pheasants Through the Third Generation."  J.W.M.
     38(2): 320-330.

David, D0J0  et al.  1955.  "Lead Toxicity in Tobacco Resembles
     an Early Sympton of Frenching."  Journal of the Australian
     Institute of Agricultural Science.  21: 182-185.

Davis,  et al., eds.  1971.  Infectious and Parasitic Disease
     of Wild Birds.  Iowa State University Press.

Davis, et al.  1970.  Infectious Diseases of Wild Mammals.   Iowa
     State University Press0

Dawson, C000  et al.  1976.  "Air Sac Renal Mucos Mycosis in an
     African Gray Parrot„"  Avian Pis,  20(3): 593-600.

DeJong, J«C.  et al.  1974.  "Inactivation of Encephalocarditis
     Virus in Aerosols."  Journal of Applied Microbiology.   27(1):
     57-65.

DeLong, R.L.  et al.  1973.  "Premature Births in California Sea
     Lions:  Association with High Organochlorine Pollutant  Resi-
     due Levels."  Science.  181: 1168-1170.

Demartini, J0C.  and Davies, R.B.  1977.  "An Epizootic of
     Pneumonia in Captive Bighorn Sheep Infected with n
     sp."  J.W.D.  13(2): 117-124.

DeOng, E.R.,  1956.  Chemistry and Uses of Pesticides.  2nd ed.
     Reinhold Publishing Corp.  New York, 334 pp.

Dilworth, T0G0  et al.  1972. "DDE and Eggshell Thickness in New
     Brunswick Woodcock."  J0W.M0  36(4): 1186-1193.

Dimmick, R.L,,  1965.  "Rhythmic Response of Serratia Marcescens
     to Elevated Temperatures."  Journal of Bacteriology.  89:
     791-798.

Dindal, D«,L0  1970. "Accumulation and Excretion of Cl36 DDT  in
     Mallard and Lesser Scaup Ducks."  J.W.D,  34(1): 74-92.

Domermuth, CoH.,  et al.  1975.  "Experimental Reproduction and
     Antibody Inhibition of Marble Spleen Disease of Pheasants."
     J.W.D,  11(3): 338-342.

Duncan, R0M0  and Jensen W.I.  1976.  "A Relationship Between
     Avian Carcasses and Living Invertebrates in the Epizootiology
     of Avian Botulism."  J.W.D.  12(1): 116-126.

Dvorn, P..  " Vi.lcox, ". 1972.  "Treated Sewage for Power Plant
     Make-up Water."  Power Engineer, November, pp. 40-41.

                                185

-------
Eckert, J.W.  1962.  "Fungistatic and Phytotoxic Properties of
     Some Derivations of Nitrobenzene."  Phytopath.  52: 642-649,

Edens, F.W.  et al.  1976.  "Effect of Dietary Lead on Repro-
     ductive Performance in Japanese Quail."  Toxicological
     Applied Pharmacology.  38(2): 307-314.   (Abs.)

Edwards, C.A.  1970.  Persistant Pesticides in the Environment.
     CRC Press, Cleveland.

Elliott.  1951.  Manual of Bacterial Plant Pathogens.  Chronica
     Botanica Co., Waltham.

Erlich, R. and S. Miller.  1968.  "Survival of Pasteurella Tula-
     rensis at Different Atmospheric Temperatures."  IIT.  Report
     BLTM 143.  Fort Detrick, Maryland, December 1968.

Eroschenko, V.P.  and Place T,A.  1977. "Prolonged effects of
     Kepone on Strength and Thickness of Eggshells from  Japanese
     Quail Fed Different Calcium Level Diets."  Environmental
     Pollution.  13(4): 255-264.

Exon, J.H.  et al.  1975.  "Hexamithiasis in Cadmium Exposed Mice."
     Architectural Environmental Health.  30(9): 463-464.  (Abs.)

Faber, P0A.  et al.  1972.  "Organochlorines and Mercury in Com-
     mon Egrets and Great Blue Herons."  Environmental Pollution.
     3(2): 111-122.

Farrington, D.O.  and Jorgenson, R.D.  1976.  "Prevalence of
     Bordetella Bronchiseptica in Certain Wild Mammals and Birds
     in Central Iowa."  J.W.D,  12(4): 523-525.

Fergeson, D.B0  1976.  "The Effect of Low Doses of Flouride on
     Enzyme Activity in Rabbit Serum."  Architectural Oral Bio-
     logy.  21(7): 449-450. (Abs.)

Fergeson, D.B.  19760  "The Effect of Low Doses of Flouride on
     Tissue Enzyme Activity in the Rat."  Architectural Oral Bio-
     logy.  21(7): 447-448.   (Abs.)

Fimreite, N. and Karstad, L.  1971.  "Effects of Dietary Methyl
     Mercury on Red-tailed Hawks."  J.W.M.  35(2): 293-300.

Finley, M.T.  et al.  1976.  "Sub-lethal Effects of Chronic Lead
     Ingestion in Mallard Ducks."  Journal of Toxicological
     Environmental Health.  1(16): 929-937.   (Abs.)

Fleet, R.R.  et al.  1972.  "Residues of DDT and Dieldrin in
     Snakes from two Texas Agro-Systems."  Bioscience.  22(11):
     664-665.

Flinckinger, E0L.  and King, K.A.  1972.  "Some Effects of Aldrin
     Treated  Rice  on Gulf Coast  Wildlife.   J.W.M.  36(3): 706-727.


                               186

-------
Foster, P0M0  1974.  "Droplet Growth Inside and Outside Cooling
     Towers -II,,"  Atmospheric Environment.  8(4): 393-402.

Fox, G.A.  19760  "Eggshell Quality: It's Ecological Physio-
     logical Significance in a DDE-Contamination Common Tern
     Population."  Wilson Bulletin.  88(3): 459-477.

Frank, P.A.  et al.  1961.  "Techniques for Evaluating Aquatic
     Weed Herbicides."  Weeds.  9: 515-521.

Friend, M. and Trainer, D0O.  1974.  "Experimental DDT Duck Hep-
     atitue Virus Interaction Studies."  J.W.M.  38(4): 887-895.

Friend, M. and Trainer, D.O.  1974,  "Experimental Dieldrin-Duck
     Hepatitus Virus Interaction Studies.  J.W.M.  38(4):  896-902,

Gall, 00Wo  and R0M0  Barnette.  1940.  "Toxic Limits of  Repla-
     cable Zinc to Corn and Cowpeas Grown on Three Florida Soils."
     Journal American Society of Agronomy.  32: 23-32.

Ganje, T0J0  Selenium, pp. 394-404, In: Diagnostic Criteria for
     Plants and Soils.  H<,D0  Chapman ed.  University of  Califor-
     nia .

Garber, K.  1967.  "Luf tverunreiniguag."  Gebr'uder Borhtraeger,
     Berlin.  pp. 152-163,,

Garber, K.  1973,,  "Luf tverunreinigugen, eine Literaturubersicht."
     Beru Eidg. Anst. Forst. Versuchsw.  102.

Gasaway, W0C,  and Buss, Io00  1972.  "Zinc Toxicity in the Mal-
     lard Duck."  J.W0M,  36(4): 1107-1117.

Gauch, HoG,  et al.  1972.  "Potential Effects of Salt Drift  from
     Cooling Towers on Vegetation and Soils."  Enclosure  to Syn-
     opsis of the Environmental Evaluation of the Proposed Bran-
     don Shores Power Plant, Johns Hopkins, University, Maryland.

Geldreich, E. and D. Van Donsel, 1970.  "Salmonellae in Fresh
     Water Pollution."  Proc. National Specialty Conference on
     Disinfection.  pp. 499-500.

Geluso, K.No  et al.  1976.  "Bat Mortality: Pesticide Poisoning
     and Migratory Stress."  Science.  194: 184-186.

Giles, R.Co  et al.  1974.  Klebsiella Air Sacculitis in  the  Owl .
     Monkey  (Aotes trivirgatus)."  Lab Animal Science.  24(4):
     610-616.

Gilman, A.P0  et al.  1977.  "Reproductive Parameters and eff
     Contaminant Levels of Great Lakes Herring Gulls."  JaW0M.
     41(3): 458-468.
                               187

-------
Goetz, A.  1954.  Early Detection of Bacteria Growth.   In:  Pro-
     ceedings of the Atmospheric Biology Conference.   University
     of Minnesota.

Goldberg, L.J0  1977.  Personal communication.

Goldberg, L.J0  and W.R. Lief.  1951.  Use of R/A Tagging  in  Dis-
     tribution of Bacteria  in Mice.  5th Research Status Report.
     Naval Biological Laboratory.  Oakland, California.

Gordon, G.E.  et al.  1973.  "Abnormally Enriched Trace Elements
     in the Atmosphere," pp. 167-174.  In: Trace Substance  in
     Environmental Health - VII.  D.D. Hemphill, ed.   University
     of Missouri, Columbia.

Gordon, M0A<,  and Salkin, I.F.  1977,  "Dermatophilz Dermatitus
     Enzootic in Deer in New York State and Vicinity."  JeW.D.
     13(2): 184-190.

Greenhouse, G.  1976.  "The Evaluation of Toxic Effects of  Chem-
     icals in Fresh Water by Usinr Frog Embryos and Larvae."
     Environmental Pollution.  11(4): 303-316.

Guderian, R. 1977.  Air Pollution: Phytotoxicity of Acid Gases
     and It's Significance  in Air Pollution Control.   Springer
     Verlag, New York.  127 pp.

Guenzi, W0D.  and W.E. Beard.  1968.  "Anaerobic Conversion of
     DDT to ODD and Aerobic Stability of DDT in Soil."  Soil
     Science America Proceedings.  32: 522-524.

Hacking, M.A.  and Sileo, L.  1974.  "Yersinia Enterpcolitica and
     Yersinia Pseudotuberculosis from Wildlife in Ontario."J„W.D.
     10(4): 452-457.

Haghiri, F.  1973.  "Cadmium Uptake by Plants."  Journal of Envi-
     ronmental Quality.  2: 93-96.

Hall, J.E,   et al.  1971.   "Effects of Aldrin on Young Pen-reared
     Pheasants."  J,WQM.  35(3): 429-434.

Halverson,  A.W.  et al.  1962.  "Effects of Sulfur Salts on Selen-
     ium Poisoning in the Rat."  Journal of Nutrition.  77: 459-464.

Hamilton, J. et al.  1976.  "Macrophage Plasminogen Induction by
     Asbestos is Blocked by Anti-inf lamatory Steroids ."  Jop-rnal
     of Experimental Medicine.  144(6): 1689-1694. (Abs.)

Hardy,  J.L.  et al.  1974.  "Wild Mammals as Hosts of Group A
     Arboviruses in Kern County, California: A Serologic and
     Virologic Survey."  American Journal of Tropical Medical
     Hygiene.

                                 188

-------
Harper, G.JL  1961.,  "Airhorne Microorganisms Survival Test with
     Four Viruses."  Journal of Hygiene.  59: 479-486.

Hartzell, A.  1929.  "Tolerance of Different Species and Varieties
     of Plants to Napthalene Vapor."  Journal of Economic Ento-
     mology.  22: 354-360.

Haynes, R.J.  1972.  "Effects of DDT on Glycogen and Lipid Levels
     in Bobwhites."  J.W.M0  36(2): 518-523.

Hays, Hu and K.W0 Rosebrough.  1972.  "Pollutant  Concentrations
     in Abnormal Young Terns from Long  Island Sound."  AUK.
     89(1): 19-35.

Hayward, H.E.  and L. Bernstein.  1958.  "Plant-Growth Relation-
     ships on Salt Affected Soils."  Botany Review.  24: 584-635.

Heath,  R.G0  1969.  "Nationwide Residues of Organochlorine Pest-
     icides in Wings of-Mallards and Black Ducks."  Pesticide
     Monitor Journal,  3(2): 115-123.

Heck, W.W.  and E.GU Pires.  1962.  Growth of Plants Fumigated
     with Saturated and Unsaturatud Hydrocarbon Gases and Their
     Derivatives.Texas Agricultural ExperimentStation,Agri-
     cultural Medical College, Texas, MP-603, 12 pp.

Heck, W.W.  et al.  1970.  "Other Phytotoxic Pollutants," pp. Fl-
     F29.   In: J. S. Jacobson and A.C0  Hill, eds. Recognition of
     Air Pollution Injury to Vegetation; A Piptoral Atlas.In-
     formative Report 1,TR-7 Agricultural Committee,Pittsburgh.
     Air Pollution Control Association, 1970.

Heinz,  GUH.  1976.  "Methylmercury: Second Generation Reproductive
     and Behavior Effects on Mallard Ducks."  J.W..M,  40(4): 710-
     715.

Heinz,  G0H.,  1976.  "Methylmercury: Second Year Feeding  Effects
     on Mallard Reproduction and Duckling Behavior."  JLW.M.
     40(1): 82-90.

Hemphill, DUD0  et al.  1973.  "Toxic Heavy Metals  in Vegetables
     and Forage Grasses in Missouri's Lead Belt."   Journal Asso-
     ciation of Analytical Chemistry.   56: 994-998.

Hewitt, E.J.  1952.  Sand and Water Culture Methods Used in  the
     Study  of Plant Nutrition.  Commonwealth Agricultural Bureaux.

Hewitt, E.J.,  and T0A. Smith.   1975.  Plant Mineral Nutrition.
     Wiley  and Sons, New York.

Hickey, J.L.S. and P.C. Reist.  1975.   "Health  Significance  of
     Airborne Microorganisms from  Wastewater Treatment  Processes.
     Part  I: Summary of  Investigations. Part  II: Health Signif-r
     icance and  Alternatives  for  Action."  Journal  Water Pollution
     Conlrol Federation.   47(12):  2741-2773.

                                189

-------
Hildebrand, S0G0  et al.  1976.  "The Potential Toxicity and Bio-
     accumulation in Aquatic Systems of Trace Elements in Aqueous
     Coal Conversion Effluents.™ In: Trace Substances in Environ-
     mental Health.  D0D0  Hemphill, ed.  University of Missouri,
     Columbia.  pp. 305-313.

Hill, E.F.  1972.  "Avoidance of Lethal Dietary Concentrations
     of Insecticide by House Sparrows."  J.W.M.,  36(2): 635-639.

Hindawi, T0J0  et al.  1976.  Ecological Effects of Aerosol Drift
     from a Saltwater Cooling System.  EPA-600/3-76-078.  USEPA,
     Washington, DC.

Hiroa, Y.  and C.C. Patterson.  1974.  "Lead Aerosol Pollution  in
     the High Sierra Overrides Natural Mechanisms Which Exclude
     Lead from a Food Chain."  Science.  184: 989-992.

Hitchcock, A.E. and P.W, Zimmerman.  1957.  "Toxic Effects of
     Vapors of Mercury and of Compounds of Mercury on Plants."
     Annual N.Y. Academy of Sciences.  65: 474-497.

Hoagland, M.B.  1952.  "Beryllium and Growth. II0 The Effects of
     Beryllium on Plant Growth."  Architectural Biochemistry
     Biophysics.  35: 249-258.

Hofstad.  et al, eds.  Diseases of Poultry.

Holmberg, J0D0  and 00L. Kinney,  1973.  Drift Technology for
     Cooling Towers.  Manley Co.

Hubert, W0T0  1972.  "Yersinosis in Mammals and Birds in the
     United States/Case Reports and a Review."  American Journal
     of Tropical Medicine Hygiene.

Huckabee, J.W,,  et al.  1972.  "Distribution of Mercury in Pheasant
     Muscle and Feathers."  J.W,M»  36(4): 1306-1309.

Huckabee, JCW.  1973.  "Mosses: Sensitive Indicators of Airborne
     Mercury Pollution,,"  Atmospheric Environment.  7: 749-754,,

Hudson, B0W0  and Quan, T0J0  1975.  "Serologic Observations
     During an Outbreak of Rat Borne Plague in the San Francisco
     Bay Area of California."  J.W.D. 11(3): 431-436.

Huff, FoA0  1972.  "Potential Augmentation of Precipitation from
     Cooling Tower Effluents."  Bulletin on the American Metero-
     logical Society.  53(7): 639-644.

Hund-Karrer, A.M.  1937.  "Comparative Toxicity of Selenates and
     Selenites to Wheat."  American Journal of Botany.  24: 720-
     728.

                                 190

-------
Hund-Karrer, A0M.  1933.  "Inhibition of Selenium  Injury to Wheat
     Plants by Sulfur."  Science.  78: 560.

Hund-Karrer, A.M.  1934.  "Selenium Injury to Wheat Plants and its
     Iniiibition by Sulphur."  Journal of Agricultural Research.
     49: 343-357,

Hutchinson, H.B, and N.H0J. Miller,  1912.  "The Direct Assim-
     ilation of Inorganic and Organic Forms of Nitrogen by Plants."
     Journal of Agricultural Science„  4: 282-302.

Hyde, K.M0  et al.  1973.  "Reproductive Success of Mallard Ducks
     Fed Mirex."  J.W.M..  37(4): 479-484.

Iltis,  J.Po  et al.  1977.  "Demonstration of an Avian Adenovirus
     as the Causative Agent of Marble Spleen Disease."  American
     Journal of Veterinary Research.  38(1): 95-100.

Ireland, M.P.  1977.  "Lead Retention in Toads, Xenopus laevis Fed
     Increasing Levels of Lead-Contaminated Earthworms."  Environ-
     mental Pollution.  12(2): 85-92.

Janda,  J. and Bosseova, M.  1970.  "The Toxic Effect of Zinc Phos-
     phide Baits on Partridges and Pheasants."  J,W.M0  34(1): 220-
     223.

Jefferies, D.J.  and French, M.G.  1972.  "Changes  Induced in the
     Pigeon Thyroid by p,p'-DDE and Dieldrin."  J.W.M.  36(1): 24-
     30.

Jefferies, D.J.  and French, M.C.  1971.  "Hyperthyroidism and
     Hypothyroidism in Pigeons Fed DDT: An Explanation for the
     "Thin Eggshell Phenomenon."  P^M.J.

Jeffries, D.J0  and M.C., French.  1972.  "Lead Concentrations in
     Small Mammals Trapped on Roadside Verges and  Field Sites."
     Environmental Pollution.  3(2):  147-156.

Jensen, M.M.  1964.  "Inactivation of Airborne Viruses by U.V.
     Irradiation."  Journal of Applied Microbiology.  12(5): 418-
     420.

Johnson.  et al.  1976.  "The Effects of Orchard Pesticide Appli-
     cations on Breeding Robins."  Wilson Bulletin.  88(1): 16-35.

Johnston, D0W0  1975.  "Organochlorine Pesticide Residues in Small
     Migratory Birds."  Pesticides Monitoring Journal.   (9)2: 79—
     88.

Jolley, Robert.  1978.  Personal Communication.


                                  191

-------
Kacew, S.Z.  et al.  1976.  "Comparison of the Subacute effects of
     Cadmium Exposure Upon Nucleic Acid, Cyclic Adenosine 3', 5'
     Monophosphate and Polyamine Metabolism in Lung and Kidney
     Cortex."  Toxicological Applied Pharmacology.  38(1): 145-156.
      (Abs.)

Kapoor, !„?„  et al.  1972.  "Comparative Metabolism of DDT, Meth-
     oxychlor and Ethoxy in Mouse, Insects and in Model Ecosystem."
     Journal of Agricultural Food Chemistry.  20:1- .

Karlsson, B.  et al.  1974.  "Locomotory and Dehydrogenase Acti-
     vities of Redstarts Phoenicurus phoenicurus L. (Aves) Given
     PCS and DDT."  Environmental Pollution.  7(1) :, 53-64.

Karstad, L.  1971.  "Tyzzer's Disease in Muskrats."  J.W.Do  7(2):
     96-99.

Karstad, L.  et al.  19750  "Hepatitis in Skunks Caused by the
     Virus of Infectious Canine Hepatitis."  JoW0D. 11(4): 494-496.

Kirk and Bistner.  Handbook of Veterinary Procedures and Emergency
     Treatment.

Klein, W. 1972.  "Metabolism of Insecticides in Microorganisms
     and Insects."  In:  Environmental Quality and Safety Vol.  I.
     F. Coulston, and F. Korte,eds.Academic Press,New York.

Klukas, R.W.  and Locke, L.N.  1970.  "An Outbreak of Fowl Cholera
     in Everglades National Park."  J.W.D.  6(1): 77-79.

Knight, L.A.,  Jr.  and E0J.  Harvey, Jr.  1974.  "Mercury Residue
     in the Common Pigeon  (Columbia livia) from Jackson, Missis-
     sippi Area, 1972."  Pesticides Monitor Journal.  8(2): 102-
     104.

Knowles, F.  1945.  "The Poisoning of Plants by Zinc."  Agricul-
     tural Progress.  32: 23-32.

Kolaja, G.Jo  and Hinton, D.E.  1976.  "Histopathologic Alterations
     in Shell Gland Accompanying DDT-Induce Thinning of Eggshell."
     Environmental Pollution.  10(3): 225-232.

Kolflat, T.D.   1974.  "Cooling Power Practices."  Power Engineering.
     12: 32-39.

Koller, L.D.  and Thigpen, J.Ee  1973,  "Bipphenyl-Exposed Rabbits."
     American Journal Veterinary Res.  34(12): 1605-1606.

Koopman, J.P.   and Janssen, F.G0J.  1975.   "The Occurrence of Sal-
     monellas  in Laboratory Animals and a  Comparison of Three
     Enrichment Methods Used in their Isolation."  Z.  Versuch-
     stierkd.   17(3):  155-158.
                                  192

-------
Korschgen, L. J.  1971.  "Disappearance and Persistence of Aldrin
     After Five Annual Applications."  J.W.M.  35(3): 494-500.

Kranz, W.C0  et al.  1971.  "Organochlorine and Heavy Metal Resi-
     dues in Bald Eagle Eggs."  Pesticide Monitor Journal.  4(3):
     136-140.

Krapo, G.L.  et al.  1973.  Mercury Residues in Pintails Breeding
     in North Dakota."  J.W.M.  37(3): 395-399.

Kubota, J. and W.H0 Allaway.  1972.  "Geographic Distribution of
     Trace Element Problems."  In:  Micronutrients in Agriculture,
     J0J. Mortredt, P<,MC Giordano, and W0L0 Lindsay, eds „  Soil
     Science Society of America, Madison, Wisconsin.

Kunz, T.H.  et al.  1977,  "Mortality of Little Brown Bats Fol-
     lowing Multiple Pesticide Applications „"  J0W.M<.  41(3): 476-
     483.

Lagerwerff, JCV.  and A0W0  Specht„  19700  "Contamination of
     Roadside Soil and Vegetation  with Cadmium, Nickel,  Lead and
     Zinc."  Environmental Science and Technology.   4: 583-586,,

Lagerwerff, J<,V0  1972,  1972.  "Lead, Mercury, and  Cadmium as
     Environmental Contaminants, pp. 593-636."  In:  Micronutrients
     in Agriculture, J.J. Mortvedt et al, eds.  Soil Science Soc-
     iety of America, Madison, Wisconsin,,

Lagerwerff, J.V0  1971.  "Uptake of Cadmium, Lead and Zinc by
     Radish from Soil and Air,,"  Soil Science.  Wl:  129-133o

Lawrence, R0M0  et al.  1976.  "Reproducible Method  for  Induction
     of Pulmonary Coccidioidomycosis in  Mice,"  Journal  of Infec-
     tious Disease.  135(1):  117-119.   (Abs.).

Leffler, E. and Y. Kott,  1974.  Virus Retention and Survival  in
     Sand.   In:  Proceedings  for Center  for Research in  Water
     Resources Symposium # 7.  84-91.  University of Texas, Austin,
     Texas„

Leif, W0R<,  and J0E0 Hebert.  1977.  Personal  Communication.
     Naval Biological Laboratory,  Oakland, California.

Leung, M.K.  et al.  1975.  "Natural  Infections of Richardson's
     Ground Squirrels with Western Equine Encephalomyelitis Virus,
     Saskatchewan, 1964-1973."  Canadian Journal Microbiology.
     21: 954-958.   'Abs.)

Leung, M.K.  et al.  1976.  "Subcutaneous Exposure of the  Richard-
     son's Ground Squirrel  (Spermophilus Sabine) to  Western Equine
     Encephalomyelitis Virus."  J0W,D.   12(2): 237-246.

                                  193

-------
Levine, H0B0  1977.  Personal Communication.  Naval Biological
     Laboratory.

Lewis, E.G.  1974.  "On the Question of Airborne Transmission of
     Pathogenic Organisms  in Cooling Tower Drift."  In: Proceedings
     of the Annual Meeting of the Cooling Tower Institute.

Lewis, Barbara Ann G.  1978.  Personal Communication.

Lewis, E. et al.  1975.  "Public Health and the Urban Grey  Squirrel:
     Mycology."  J.W..D..  11(4): 502-504.

Libke, K.G0 and Walton, A.M0  1975.  "Presumptive Paratuberculosis
     in a Virginia White-Tailed Deer."  J.W0D0  11(4):  552-553.

Licht, L.E.  1976.  "Postmetamorphic Retention of C   DDT by Wood
     Frog Tadpoles."  Comprehensive Biochemical Physiological
     Comprehensive Pharmacology.55(2): 119-121. (Abs.)

Lichtenstein, E.P0 and J0R0 Corbett.  1969.  "Enzymatic Conversion
     of Aldrin to Dieldrin with Subcellular Components  of Pea
     Plants."  Journal of Agricultural Food Chemistry.  17: 589-594.

Liebig, G.F., Jr.  1966.  Arsenic pp. 13-23.  In: Diagnostic Cri-
     teria for Plants and Soils.  H0Do Chapman ed.  University of
     California.

Lighthart, Bruce.  1972.  "Survival of Airborne Bacteria in a High
     Urban Concentration of Carbon Monoxide."  Journal  of Applied
     Microbiology.  25(8)

Lighthart, B.  et al.  1976.  "Bacteria and Viruses."   In: Eco-
     logical Systems Approaches to Aerobiology.  R0L0 Edmonds,
     ed.   Donden, Hutchinson and Ross0  Stroudsburg, Pennsylvania.

Linder, R.L0  1970.  "Residues in the Brain of Adult Pheasants
     Given Dieldrin."  J0W0M0  34(4): 954-956.

Longcore, J0R0  and Samson, F_B0  1973.   "Eggshell Breakage by
     Incubating Black Ducks Fed DDE."  J,W,M0  37(3): 390-394.

McDougle, H«E. and Vaught, R0W0  1968.  "An Epizootic of Aspergil-
     losis in Canada Geese." Journal of Wildlife Management.  32:
     415-417.

McDuffie, B.  et al.  1976.  "Trace Metals in Rivers -  Speciations,
     Transport and Role of Spediments."  In: Trace Substances in
     Environmental Health.  D0D. Hemphill ed.  University of Mis-
     souri, Columbia.  pp0  85-950

McMurtrey, J0E0, Jr.  19320  "Effect of Thallium on Growth of
     Plants,,"  Science. 78:86.

                                 194

-------
Magos, L0  and Webb, M0  19760  "The Interaction Between Cadmium,
     Mercury and Zinc Administered Subcutaneously in a Single
     Injection."  Architectural Toxicology.  36(1): 53-62.   (Abs . )

Mahoney, J0J0, Jr.  1975.  "DDT and DDE Effects of Condition in
     White-Troated Sparrows,,"  J.W0M0  39(3): 520-527,

Mangelson, N0F0  et al«  19750  "Trace Element Analysis for the
     Environmental Baseline Studies of the Navajo-Kaiparowits
     Generation Stations, pp. 369-377,"  In: Trace Substance in
     Environmental Health - VII .   D0D0 Hemphill, ed.  University
     of Missouri, Columbia „

Marth, E0H0  19650  "Residues and Some Effects of Chlorinated
     Hydrocarbon Insecticides in Biological Material."  Residue
     Reviewo  9: 1-890

Martin, A. and F0R0 Barber.  1974.  "Some Water Droplet Measure-
     ments Inside Cooling Towers."  Atmospheric Environment.
     8(4): 325-336.

Martin, H.  1961.  Guide to the Chemicals Used in Crop Protection.
     4th ed.  Research Branch, Canada Department of Agriculture,
     Ottawa.

Martyny, J.W0  and Botzler, R0G0   1975.  Listeria monocytogenes
     Isolated from Wapiti (Cervus canadensis roosevelti) .  J. W.D.,
     11(3): 330-334.

Martyny, J0W.  and Botzler, R0G0   1976.  "Yersinae Isolated from
     Wapiti."  JoW.D,  12(3): 386-389.

Mayer, H. and Werner, F.  1974,,  "Bacterial Investigations on
     Reptiles and Amphibians."  Medical Mikrobiology Parasitology .
     229(4): 470-481.  (Abs»)

Menzie, C0M0  1972.  "Fate of Pesticides in the Environment."
     Annual Revised Entanology.   17: 199-222.

Mettler, F.  1975.  "Generalized Protothecosis in a Bat (Pteropus
     lylei) .  Veterinary Pathology.  12(2): 118-124.   (AbsTT

Meyer, M.E.  19760  "Evolution and Taxonomy in the Genus Brucella :
     Brucellosis of Rodents.  Theriogenology .  6(2/3): 263-272.
Migaka, G0 and Seibold, H0R.  1976.  "Dermatophilosis  in a Titi
     Monkey  (Callibebus moloch) .  American Journal of  Veterinary
     Research"  37(10): 1225-1226 .

Migake, G. and Frye, F0L.  1975.  "Mycotic Granuloma in a Tiger
     Salamander."  J.W.D.  11(4): 525-528.
                                 195

-------
Moon, H.W.  et al.  1974.   "Intraepithelial Vibrio Associated with
     Acute Typhlitis of Young Rabbits."  Veterinary Pathology,,
     11(4); 313-326.  (Abs.).

Morrison, W.I.  et al.  1976.  "An Immunopathologic Study of
     Interstitial Nephritis Associated with Experimental Canine
     Adenovirus Infection."  J. Pathology.  120(4): 221-228.

Morse, E0V0  et al.  1976.  "Salmonellosis in Equidae: A Study of
     23 Cases."   (Abs.).

Moteyunas, LCI.  and Ezerskene, E.P.  1974.  Small Mammals  in
     Natural Foci of Human  Disease in the Lithuanian SSP Zoology Zh.
     53(10): 1580-1583. (Abs.).

Motto, H.L.  et al.  1970.  "Lead in Soils and Plants: It's Rela-
     tionship to Traffic Volume and Proximity to Highways."  Envi-
     ronmental Science and  Technology.  4: 231-238.

Moxon, A0L,,  1938.  "The Effect of Arsenic on the Toxicity  of
     Seleniferous Grains."  Science.  88:81.

Mulhern, B.M.  1970.  "Organochlorine Residues and Autopsy  Data
     from Bald Eagles."  Pestici.   Monitor Journal.  4(3):  141-144.

Mullins, W0H.  1977.  "Effects of Phenyl Mercury on Captive Game
     Farm Pheasants."  J.W.M,  41(2): 302-308.

National Academy of Science 1977.  Airborne Particle,  National
     Academy of Science, Washington, D.C.

National Academy of Sciences 19740  Chromium.  National Academy
     of Science, Washington, D0C0  155 pp.

National Academy of Science.  1977.  Fates of Pollutants; Research
     and Development ^eeds.  National Academy of Science, Washing-
     ton, D.Co

National Academy of Science.  1972 Lead; Airborne Lead in Perspective.
     National Academy of Science, Washington, D.C.  330 pp.

National Academy of Science.  1975.  Nickel.  National Academy of
     Science, Washington, D.C.

National Academy of Science.  1976.  Selenium.  National Academy
     of Science, Washington, D.C.

National Research Council, Agricultural Board, Committee on Animal
     Nutrition; Subcommittee on Selenium, 1971.  Selenium in Nutri-
     tion.  National Academy of Science, Washington, D.C0   79 pp.

Natusch, D.F.SU  et al.  1974.  "Toxic Trace Elements: Preferren-
     tial Concentr?tion in Respirable Particles."  Science.  183;
     202- .
                                 196

-------
Neiland, K0A,  1970.  "Rangiferine Brucellosis  in Alaskan Canids."
     J.W.D,  6(3):  136-139.

Nester, D.M.  1972.  Salt Water Cooling Tower.   In: Cooling Towers.
     American Institute of Chemical Engineers,  New York.

Newman, M<,SC  et al.  19750  "Dermatophilosis in two Polar Bears."
     Journal of American Veterinary Medicine Association.  167(7):
     561-564.

Nicholas, D0JoD0  and W0D<,Ec Thomas.  1954.  "Some Effects of
     Heavy Metals on Plants Grown in Soil Culture Volume II.  The
     Effect of Nickel on Fertilizer and Soil Phosphate Uptakes
     and Iron and Nickel States of Tomato."  Plant and Soil.
     5: 182-

Nickerson, P.,R0  and K0R0 Barbehenn.  1975.  "Organochlorine Resi-
     dues in Starlings."  Pesticides Monitor Journal.  8(4): 247-
     254.

Nus Corp.  1974.  "Potential Virus Hazards from Cooling Towers
     Operated with  Polluted Surface Water."  Public Service Elec-
     tric and Gas Company, Newark.

Ohbayashi, M.  1971.  "Mucormycosis in Laboratory Reared Rodents."
     J.W,D0  7(1):  59-62,

Ohi, et al.  1975.,  "Interaction of Dietary Methyl Mercury and
     Selenium on Accumulation and Retention of  These Substances  in
     Rat Organs."   Toxicology Applied Pharmacology.  32(3): 527-
     533.  (Abs.).

Oliver, E.G.  1973.  "Heavy Metal Levels of Ottawa and Rideau
     River Sediments."  Environmental Science and Technology.
     7: 135-137.

Owens, DoR«,  et al.  1975.  "Naturally Occurring Histoplasmosis
     in  the Chinchilla  (Chinchilla laniger).   Journal Clinical
     Microbiology.  1(5): 486-488.  (Abs.).

Parker, JCW0  1976.  "Pesticides and Eggshell Thinning in the
     Mississippi Kite."  J0W0M0  40(2): 243-248.

Parks, J.B.  et al.  1972.  "Parainfluenza 3 Virus Infection in
     Rocky Mountain Bighorn Sheep."  Journal American Veterinary
     Medical Association.  161: 669-672.

Parizek, J.  et al.  1971.  "The Detoxifying Effects of Selenium
     Interrelations Between Compound  of Selenium and Certain
     Metals."  pp.  85-122.  In: New Trace Elements in Nutrition.
     W. Mertz and W.E. Cornatzer, eds, Marcel Dekker, Inc., New
     York.
                                 197

-------
Patton, NoM0  1975.  "Cutaneous and Pulmonary Aspergillosis  in
     Rabbitso"  Lab Animal Science.  25(3): 347-350.

Peakill, D.B.  1970. "p,p'-DDT: Effect on Calcium Metabolism and
     Concentration on Estradiol in the Blood."  Science,   168:
     592-594.

Peakill, D.B.  et al.  1975.  "Blood Calcium Levels and the  Mech-
     anism of DDE-Induced Eggshell Thinning."  Environmental Pol-
     lution.  9(4): 289-294.

Peakill, D.B.  and K0J0  Lovett.  1972,  "Mercury  : Its Occurrence
     and Effects in the Ecosystem."  Science,  22: 20-25.

Peakill, D0B0  et al.  1975.  "Organochlorine Residues  in  Alaskan
     Peregrines."  Pesticides Monitor Journal.  8(4): 255-260.

Pearce, P.A0  et al.  1976.  "Mercury in Waterfowl from Eastern
     Canada,"  J0W0M0  40(4): 694-703=

Pell, J.  1974.  "The Chalk Point Cooling Tower Project."  In:
     Cooling Tower Environment-1974.  Proc. of Symposium Power
     Plant Siting Program, Maryland,

Peterson, S0R.  and Ellarson, R0S0  1976,  "Total Mercury  Residues
     in Livers and Eggs of Oldsquaws,"  J.W..M.,  40(4): 704-709.

Petrova, M0A.  et al,  1974.  "Experimental Purulent Meningitis
     and its Treatment with Antibiotics,"  Antibiotiki  (Mosc,),
     19(5): 422-427.  (Abs.).

Porter, K.R0  and Hakanson, D,E0  1976.  "Toxicity of Mine Drainage
     to Embryonic and Larval Boreal Toads (Bufonidae: Bufo boreas),

Press,  M,P0  1977.  "Lead Encepnalopathy in Neonatal Long-Evans
     Rats; Morphologic Studies."  Journal of Neuropathology  Exp-
     erimental Neurology.  36(1): 169-193.  (Abs.)

Prior,  M0G0  1976.  "Isolation of Brucella abortus from Two  Dogs
     in Contact with Bovine Brucellosis."  Canadian Journal  of
     Comprehensive Medicine.  40(1): 117-118.  (Abs.).

Ramirez, R.  et al.  1976.  "Mycotic Flora in the Lower Digestive
     Tract of Feral Pigeons  (Columbia livia) in the El Paso,
     Texas Area."  J,WBD0  12ll): 83-85.

Rapp, J.P.  and McGrath, J,T,  1975,  "Mycotic Encephalitis  in
     Weanling Rats."  Lab Animal Science".  25(4): 477-480.

Rastogi, S,Ca  and Clausen, J.   1976,  "Absorption of Lead Through
     the Skin."  Toxicology,  6(3): 371-376.  (Abs.).

                                198

-------
Ray, T.L0  and Wuepper, K.D0  1976.  "Experimental Cutaneous Con-
     didiasis in Rodents."  Journal of Investigative Dermatology„
     66(1): 29-33,

Reedj R0E0  et al.  1976.  "Coccidioidomycosis in a California
     Sea Lion."  J0W,D,  12(3): 372-375.

Reichel, W.L0  1969.  "Pesticide Residues in Eagles."  Pesticide
     Monitor Journal„  3(3): 142-144.

Reichenback-Klinke, H. and Elkan, E.  1965.   Principle Diseases
     of Lower Vertebrates: Diseases of Amphibians.   Academic
     PressInc.(London)0

Reichenback-Klinke, H. and Elkan, E0  1965.   Principal Diseases
     of Lower Vertebrates: Diseases of ReptilesTAcademic Press
     Inc „  (London) .                          ~~~

Reidinger, R0F0  et al.  1976.  "Organochlorine Residues  in Adults
     of Six South-Western Bat Species."  J.W.M.  40(4): 677-680.

Reidinger, R0F0, Jr.  and D0G, Crabtree.  1974.  "Organochlorine
     Residues in Golden-Eagles."  United States March  1964-July
     1971.  Pesticides Monitoring Journal.  8(1): 37-43.

Rhodin, A0G0J0  and Anver, M0R0  1977.  "Mycobacteriosis  in
     Turtles; Cutaneous and Heptosplenic Involvement in a Phrynops
     hilari.  J,W0D0  13(2): 180-183.

Rice, C.Po  and H0C.  Sikka.  1973.  "Uptake and Metabolism of DDT
     by Six Species of Marine Algae."  Journal of Agricultural
     Food  Chemistry.  21:  148-

Risebrough, R.W.   and Anderson, D.W.  1975.  "Some Effects of DDE
     and PCB on Mallards and Their  Eggs."  J.W.M.  39(3): 508-513.

Roffman, A.  et al.   1973.  The State of the Art of Saltwater
     Cooling Towers for Steam Electric Generating Plants. Westing-
     house Electric Co., Pittsburgh.

Romney, E.M0  et al,,  1962.  "Beryllium and Growth of  Bush Beans."
     Science.  135:786.

Romney, E0M0  and  JoD0 Childress»   1965.  "Effects of  Beryllium
     in Plants and Soils."  Soil Science.  100: 210-217.

Roscoe, DoEo  1975.   "Spontaneous Dermatophilosis  in Twin White-
     Tailed Deer Fawns."   J.W.D.   11(3): 398-401.

Rosen, M.N0  1964.  "Aspergillosis  in Wild and Domestic Birds."
     Avian Disease.   8;  1-5.

                                199

-------
Rowlands, D.G,  19680  "The Metabolism of DDT in Stored Wheat
     Grains."  Journal of Stored Products Research.  4: 183-196„

Ruhling, A. 1970.    Heavy Metals within the Region of Vargo-
     Trollhat~an Lund University (Sweden).  Institute of Ecological
     Botany Roi ^>rt 14,  22 pp.

Rutgers University.  1972.  Forked River Nuclear Station Unit  I
     Natural Draft Salt Water Cooling Tower„  Assessment of Envi-
     ronmental Affects.

Saez, H. and Rinjard, J.  1975.  "Candidiasis in Wild Animals  in
     Captivity,,"  Bulletin of Society of French Mycological Medi-
     cine.  4(2):  131-134.  (Abs . ) .

Safe, S.  et a!0  1976.  "The Metabolism of Chlorinated Aromatic
     Pollutants by the Frog."  Canadian Journal of Zoology..  54(11):
     1818-1823.

Salkin, I.F.  et al.  1975.  "Dermatophilosis: Increased Prevalence
     in New York State with Involvement of Wild Raccoons." 75th
     Anniversary Meeting, Americr- Society for Microbiology, p. 93.
     (Abs.).

Salkin, I.F.  et al.  1975.  "Dual Infection of a White-Tailed
     Deer by Dermatophilus congolensis and Alternaria alternata.
     Journal of American Veterinary Medicine Association.  167:
     571-573.

Sauer,  R0M<,  1966.  "Cutaneous Mucormycosis (Phycomycosis) in  a
     Squirrel (Sciurus carolinensis).   American Journal of Veter-
     inary Research., 27:380-383. (Abs.).

Scharrer, K, and W. Hofner.  1958.  "Sorption and Leachery of
     Zinc in Soil.  Zpjtschr.  Pflanz.  Em., Duengung, Bidenkunde,
     81:201-202.

Schmid, W.E.  1967.  "Influence of Thallous Ions in the Transport
     of Certain Cations by Excised Barley Roots."  In: Transcript
     of the Illinois Academy of Science.  60: 61-67.

Schroeder, H.A. and J. Balassa.  1961.  "Abnormal Trace Metals in
     Man: Cadmium."  Journal of Chronic Disability.  14: 236-258.

Schroeder, H.A. and J. Balassa,  1963.  "Cadmium Uptake by Vege-
     tables from Superphosphate by Soil."  Science.  140: 819-820.

Seidensticker, J.  1  and H.V.  Reynolds III.  1971.  "The Nesting
     Reproductive  Performance and Chlorinated Hydrocarbon Resi-
     dues in the Red Tailed Hawk and Great Horned Owl in South-
     Central Montana."  Wilson Bulletin.   83(4): 408-418.

                                200

-------
Seidler, RCJ.>  et al. 1975.  "Potential Pathogens in the Environ-
     ment"     K. Pneumoniae."  Applied Microbiology.  29(6)° 819-
     825, June 1975.

Selyankina, K0P.  1970,  "Selenium and Tellurium in the Atmosphere
     Around Electrolytic Copper Plants."  Hungarian Sanitation.
     35: 431-432,

Shell Development Co.  1958.  Process for Controlling Aquatic
     Vegetation with F-98 Aquatic Herbicide.   Modesto, California.

Shofner, F0M0  and C.,0. Thomas „  1972.  "Drift Measurements in
     Cooling Towers."  In: Cooling Towers; CEP Technical Manual,
     Chicago, American  Institute of Chemical Engineers„  pp. 125-
     130.

SileOj, L.  et al.  1977.  "Effects of Organochlorines on Ringbilled
     Gulls."  J.W.D.  13(3): 313-322.

Sileo, L. and Palmer, N.C.  1973.  "Probable Cutaneous Protothe-
     cosis in a Beaver."  J0W0D0  9(4): 320-322.

Skerfving, S. et al.  1970.  "Chromosome Breakage in Humans Exposed
     to Methyl Mercury Through Fish Consumption."  Architectural
     Environmental Health.  21: 133-139.

Smith.  1957.  A Textbook of Plant Virus Diseases.  Little, Brown
     and Co., Boston.

Smith, W0H0  1972.  "Lead and Mercury Burden on Urban Woody Plants."
     Science.  176: 1237-1239.

Snow, Jo  1955.  On the Mode of Communication of Cholera.  2nd ed.
     Churchill, London.

Snyder, R.L.  1974.  "Effects of Dieldrin on Homing and Orientation
     in Deer Mice."  J.W.M.  38(2): 362-364.

Snyder, N.F.Ro  et al.  1973.  "Organochlorjdes, Heavy Metals and
     the Biology of North American Accipiters."  Bioscience.
     23(5): 300-305.

Soltys, M.Ao  et al.  1967.  "Johne's Disease in a Moose  (Alces
     aIces) .  Bulletin of Wildlife Disease Association.  3: 183-
     184.  (Abs.).

Spann, J.W0  et al.  1972.  "Ethyl Mercury P-toluene Sulfonanilide;
     Lethal and Reproductive Effects on Pheasants."  Science.
     175: 328-331.

Spencer, E0L.  and G.I0  Lavin.  1939.  "Frenching of Tobacco."
     Phytopath.  29: 205-503.
                                 201

-------
Spencer, E0L0  1937.  "Frenching of Tobacco and  Thallium Toxicity.
     American Journal of Botany.  24:  16-24.

Spencer, EoL.  1935.  "Studies on Frenching of Tobacco."  Phytp-
     path.  25:  1067-1084.

Staker, E0V,,  1943.  Progress Report on  the Control  of  Zinc  Tox-
     icity in Peat  Soils.  Proceedings of the Soil  Science Society
     of America.  7: 787-392.

Stendell, R0C0   1976„  "Mercury in Eggs  of Aquatic Birds,  Lake
     St., Clair - 1973."  Pesticide Monitor Journal,   10(1):  7-9,

Stendell, R0C<,   1977,,  "Organochlorine and Mercury Residues  in
     Canvasback Duck Eggs, 1972-73."   J.W.M0  41(3):  453-457,

Stevenson, A,  1976.  "Effects of Subacute and Chronic  Lead  Treat-
     ment of Glucose Homeostasis and Renal Cyclic AMP Metabolism
     in RatSo"  Toxicology„  6(3): 265-275„ (Abs<,)o

Stiles, W.  1946.   Trace Elements in Plants and  Animals,.   Macmil-
     lan Co«, New York.

Stoewsand, G0S. et  al.  1971.  "Eggshell Thinning in  Japanese
     Quail Fed Mercuric Chloride/'  Science.  173:  1030-1031.

Stone, W.Bo  1972.  "Another Case of Dermatophilosis  in a  White-
     Tailed Deer."  N.Y, Fish Game Journa1.  19: 184.

Svoboda, J.  1958.  "The Industrial Poisoning of Bees."   Inter-
     national Beekeeping Congress Report.  17: 79-81.

Swaine, D<,J0  1955.  The Trace Element Content of Soils.   Common-
     wealth Bureau  Soil Science Commission.  No. 48.  Herald Prin-
     ting Works, York, England.

Swinne-Desgain, D.  1975.  "Epidemiology of Cryptococcosis."  Bul-
     letin of the Society of French Mycological  Medicine.  4(2):
     139-140"!  (Abs.).

Taylor, F0G0, Jr.   et al.  1974.  "Environmental Effects  of  Chrom-
     ium and Zinc in Cooling Water Drift/'  In:  Cooling  Tower
     Environment -  1974, Proc. of Symposium Sponsored by  Power
     Plant Siting Program, Maryland.

Temple, S0A0  1972.  "Chlorinated Hydrocarbon Residues  and Repro-
     ductive Success in Eastern North American Merlins/'   Condor.
     74(1): 105-106.

Thigpen, J0E0  et al.  1975.  "Opossums as a Reservoir  for Salmon-
     el la i."  Journal of American Veterinary Medicine Association.
     167(7): 590-592.
                                202
                                                                   IT

-------
Thill, R0E0  et a!0  1972.  "Effects  of Aldrin on Young Pheasants
     Under Semi-Natural Conditions,,"  Bulletin of Environmental
     Contamination Toxicology.  7(2/3): 188-192,

Thoen, C00,  et a!0  1977.  "Mycobacterium Avium Serotype I Infec-
     tion in a Sandhill Crane  (Grus canadensis).  JoW0D0  13(1):
     40~420

Thomas, B0  et ala  1972.  "Lead and Cadmium Content of Some Vege-
     table Foodstuffs,"  Journal of Scientific Food Agriculture.
     23"; 1493-1498.

Thorsen, J,  et al,  1977.  "Viruses Isolated from Captive and
     Free-Ranging Wild Ruminants in Alberta,"  J,W0D0  13(1): 74-79.

Thornton, I. and J0S,, Webb.  1975.  "Trace Elements in Soils and
     Surface Waters Contaminated by Past Metalliferous Mining
     in Parts of England."  pp. 77-88.  In: Trace Elements in
     Environmental Health - IX.  D0D0 Hemphill, ed.  University
     of Missouri, Columbia.

Tigertt, W0D0  et al.  1961.  "Airborne 2 Fever."  Bacteriology
     Review.  25: 285-293.

Torgeson, D0C0, ed,  1967.  Fungicides; An Advanced Treatise.  Vol.
     I.  Academic Press, New York,,

Torkelsons T0R0  et al.  1976.  "The Toxicity of Chloroform as
     Determined by Single and Repeated Exposure of Laboratory
     Animals."  American Industrial Hygiene Association Journal,
     37(12): 697-705,(Abs,).

UoSo Department of Agriculture.  1971,  Diseases of Forest and
     Shade Trees of the United States,  Handbook 386„

UoSo Department of Agriculture, Regulation Division (v.do).  An-
     notated Index of Registered Fungicides and Nematicides;
     Their Uses in the United  States,  USDA, Washington,

U.S, Army,  1968,  Aerosolization of Pastuerella Pest is,  BLTM  1400
     Fort Detrick, Maryland, August 1968,

U.S. Army.  1965,  The Effect  of Mustard on Escherichia Coli-Un-
     balanced Growth.  E0A,  CRDLR 3257,  Edgewood Arsenal Edge-
     wood, New Jersey,

Uc,S. Army.  1969.  Effect of Temperature on Serratia-Marcesens.
     Illinois Institute of Technology, Chicago, April  1969.

U.S. Army.  1967.  Enterotoxin B.  DPGRT 67102.  Dugway Proving
     Grounds, Utah.

                                203

-------
U.S0 Army.  1966.  Estimate  of Line  Source.  APGTR  66-50.   Aber-
     deen Proving Grounds, Maryland, August  1966.

U.So Army.  1953.  Field Evaluation  of Dried Bacillus  Globii  Spores.
     DPGR 351   Dugway Proving Grounds, Utah.

U.S. Army.  Influence of Relative Humidity on  the Survival  of P.
     Tularensis.  FDBLTM 50.  Fort Detrick,  Maryland,  May  1964.

U.So Army.  Long Distance Travel of  Rust Spores.  Special Report
     219 II and Supplement.   Camp Detrick, Maryland.

U.S. Army.  1965.  Osmotic Effects on the Survivability  of  Pasteur-
     ella Pestis.  BLTM 69.   Fort Detrick, Maryland.

U.S. Army.  1966.  Persistence of Pathogenic Microorganisms in
     Soil and Climate,  T67-107.  Dugway Proving Grounds, Utah,

UcS. Army.  1974.  The Question Concerning the  Inactivation of
     Viruses in Air.  FTD WPAFB MTD  24 1686.  Wright Patterson Air
     Force Base, Ohio.,
U.S. Army.  1967.  Rheological Responses of  Dry Serratia Marcescens
     to Environment Changes.  BLT...1 124„  Fort Detrick, Maryland.

U.S. Army.  1964.  Serratia  Marcescens Traced by Sarcina Lutea.
     SES 206.  Suffield Experimental Station, Canada,  15 July 1964.

U.S. Army.  19650  The Stability of Toxic Proteins.  Illinois
     Institute of Technology.   I.I.T0L.  6013-15.

UoS. Army.  1965.  Venezuelan Equine Encephalitis Studies.  Fort
     Detrick, Maryland, BLTMS 267,

U.S. Department of Interior,  Fish and Wildlife  Service.  1972.
     Comparative Diet"ry Toxicities  of Pesticides to Birds.
     Special Scientific Report - Wildlife No.  152.

U.S. Environmental Protection Agency.  1977.  Candidate  List  of
     Chemical Substances; Toxic Substances Control  Act.  3  Vol.
     USEPA, Washington.

University of Pennsylvania.   1956.   Simian Virus Studies.   Big Ben
     B156 SR6.

Van Hook, R.I.  and W.D. Shults, eds.  1977.  "Effects  of Trace
     Contaminants from Coal  Combustion."  Proceedings  of Work-
     shops.  Knoxville, Tennessee.  August 2-6, 1976.

Vancelon, A.P.    1975.  "Nickel."  In: Diagnostic Criteria  for
     Plants and Soils.  H0D.   Chapman, ed.  University  of Califor-
     nia .

                                 204

-------
Vanselon, A.P.  1966.  "Silver."  pp. 405-408.   In: Diagnostic
     Criteria for Plants and Soils.  H0D. Chapman ed.  University
     of Chicago.

Van Velzen, ACC.  et al.  1972.  "Lethal Mobilization of DDT by
     Cowbirds."  J.W.M0  36(3): 733-739.

Vermeer, K.  1973.  "Comparison of Food Habits and Mercury Resi-
     dues of Caspian and Common Terns."  Can, Field Nat.  87(3):
     305.

Von Overbeck, J.  et al0  1959.  "Acrolein for the Control of
     Water Weeds and Disease Carrying Water  Snails."  Science.
     29: 335-336.

Vermeer, K.  et al.  1973.  "Mercury in Aquatic  Birds at Clay Lake,
     Western Ontario,"  J.W0M0  37(1): 58-61.

Vermeer, K. and Armstrong, F.A0J<,  1972=  "Mercury in Canadian
     Ducks."  J,W0M,  36(1): 179-182.

Viets, F0G0  1966.  "Zinc Difficiency in the  Soil-Plant System,,"
     In: Zinc Metabolism,  A.S0 Prasod and Charles C. Thomas, eds„
     pp. 90-127.

Wahlberg, J0E0 1976,  "Percutaneous  Toxicity  of  Solvents.  A Com-
     parative Investigation in the Guinea Pig with Benzene, Toluene,
     and 1,1,2-trichloroethane."  American Occupational Hygiene.
     19(2): 115-119.   (Abs.).

Walkas R.  1976.  The Distribution of Enteric Bacterial Aerosols
     Emitted from a Small Extended Aeration  Activated Sludge
     Sewage Treatment Plant.  Masters Thesis.  CW Post College,
     New York.

Walker, G.W0R0  and K.P. Ting.  1967.  "Effect of Selenium on
     Recombination in Barley."  Journal of Genetic Cytology.  9:
     314-320.

Wallace, M0E.  1971.  "An Unprecedented Number of Mutants in a
     Colony of Wild Mice."  Environmental Pollution.  1(3): 175-184.

Ward, FcP.  et al.  1970.  "Pulmonary Aspergillosis in Praire
     Falcon Nest Mates."  J.W.D.  6(1): 80-83.

Ward, W.J0  1975.  "Cooling Water Treatment  Chemicals."  In:
     Cooling Towers, Vol. II.  American Institute of Chemical
     Engineers, New York.  1975.

Warren, H0V0  and R0E. Delavault.  1962.  "Lead  in Some Food Crops
     and Trees."  Journal of Scientific Food  Agriculture.  13:  96-
     98.
                                 205

-------
Watkins, H.M0S.  et al.   1965.  Behavior of Colorado Tick Fever,
     Vesicular Stomatitis, Neurovaccinia and Encephalomycarditis
     Viruses in the Airborne State.  In: Symposium on Aerobiology
     (R0L. Dimmick, ed.)pp. 381-388.  Naval Biological Lab,  Oak-
     land, California.

Weast,  Robert C. Ph.D.   1976.  Handbook of Chemistry and Physics.
     56th ed.  CRC Press, Cleveland.

Weinstein, L0H0  1950.   Unpublished.

Weis, J.  1975.  "The Effect of DDT on Tail Regeneration in  Rana
     Pipiens and R, Catesbeiana Tadpoles."  Copeia.   (4): 765-767.

Welch,  W0Ro  and D0L0 Dick.  1975,,  "Lead Concentrations in  Tis-
     sues of Roadside Mice,,"  Environmental Pollution.  8(1):  15-
     21.

Wellings, F0M0  et al.   1975.  "Demonstration of Viruses in  Ground
     Water."  Journal of  Applied Microbiology.  29(6):  751.

White,  D.H.  1976.  "Residues of DDT and DDE in Livers  of Water-
     fowl, Northeastern  Louisiana  1970-71."  Pesticide  Monitor
     Journal.  10(1): 2-3.

White,  F.H.  et al.  1973.  "Isolation of Edwardsiella  Tarda from
     Aquatic Animal Species and Surface Waters in Florida."  J,W0D.
     9(3): 204-208.

Wiefkin, P.  et al.  1976.  "The Metabolism of Biphenyl by Iso-
     lated Viable Rat Hepatocytes."  Xenobiotica.  6(12): 725-743.
     (Abs.).

Wiemeyer, S0N0  et al.   1975.  "Effects of Environmental Pollutants
     on Connecticut and  Maryland Ospreys."  J0W0M0  39(1): 124-139.

Williams, R.J.B,  and H.N. LeRiche,  1968.  "The Effect of Traces
     of Beryllium on the  Growth of Kale, Grass and Mustard."
     Plant and Soil,  29: 317-326.

Windholz, Martha,  ed,  1976.  The  Merck Index.  9th ed.  Merck and
     Co., InCo  Rahway, New Jersey.

Wirth,  T.  1975.  "The Effect of Asbestos Cement, Viccasbestos
     Samples and Quartz on the Peritoneum of the Mouse."  Path-
     ology Microbiology.  42(1): 15-28.  (Abs.).

Wood, J0M0  1974.   "Biological Cycles for Elements in the Environ-
     ment."  Science.  183: 1049-1054.

Woolf,  A.  et al.   1970.  "Mycoplasma Isolates from Pneumonia  in
     Captive Rocky Mountain Bighorn Sheep."  J,W,D,,  6(3): 169-
     170.
                                206

-------
Wyand, D0S0  et al,  1971.  "Aspergillosis and Renal Oxalosis in
     a White-Tailed Deer."  J,WoDo  7(1): 52-56,

Yau, E0To  and Mennear, J0H0  1977,  "The Inhibitory Effect of
     DDT on Insulin Secretion in Mice,"  Toxicological Applied
     Pharmacology,  39(1): 81-88,   (Abs,),

Yopp, JoH0  et al0  1974,  "Determination of Maximum Permissable
     Levels of Selected Chemicals that Exert Toxic Effects on
     Plants of Economic Importance  in Illinois, pp, 237-239."   In:
     Illinois Institute for Environmental Quality,  Document No.
     74-33, 272 pp,

Yuill, T.M,  and Hanson, R0Po  1964.  "Serologic Evidence of Cal-
     ifornia Encephalitis Virus and Western Equine Encephalitis
     in Snowshoe Hares."  Zoonoses Research.  3: 153-164.   (Abs.).

Zinkl, J,G0  et al.  1977.  "Aspergillosis in Common Crows in
     Nebraska, 1974."  J0W0D0  13(2): 191-193.

Zobell, CoE0  1946.  Marine Microbiology Chronica Botanica.  Wal-
     tham, Mass.

Zoller, W0H.  et al.  1974.  "Emissions of Trace Elements from
     Coal Fired Power Plants, pp. 167-172."  In: Trace Substances
     in Environmental Health VIII.  D<,D0 Hemphill ed.  University
     of Missouri, Columbia.

Zolov, C.  et al.  1967.  "Pleural Asbestosis in Agricultural
     Workers."  Environmental Research,  1: 287-292,
                                 207

-------
                                 TECHNICAL REPORT
                          (Please read Instructions on the reverse
                           DATA
                           before completing)
 1. REPORT NO.
  EPA-600/7-79-251a
                                                       3. RECIPIENT'S ACCESSION NO.
 4. TITLE AND SUBTITLE
 Effects of Pathogenic and Toxic Materials
  Transported Via Cooling Device Drift--
  Volume 1. Technical Report	
                                 5. REPORT DATE
                                  November 1979
                                 6. PERFORMING ORGANIZATION CODE
 7. AUTHOR(S)
                                                       8. PERFORMING ORGANIZATION REPORT NO.
 H;D. Freudenthal,  J.E.Rubinstein, and A. Uzzo
 9. PERFORMING ORGANIZATION NAME AND ADDRESS
 H2M Corporation
 375 Fulton Street
 Farmingdale, New York 11735
                                                       10. PROGRAM ELEMENT NO.
                                  INE624A
                                  11. CONTRACT/GRANT NO.
                                  68-02-2625
 12. SPONSORING AGENCY NAME AND ADDRESS
 EPA, Office of  Research and Development
 Industrial Environmental Research Laboratory
 Research Triangle Park, NC  27711
                                  13. TYPE OF REPORT AND PERIOD COVERED
                                  Final; 1/77 - 9/79	
                                  14. SPONSORING AGENCY CODE
                                   EPA/600/13
 15. SUPPLEMENTARY NOTES  IERL-RTP project officer is
 919/541-2915.
                           Michael C. Osborne, Mail Drop 61,
 16. ABSTRACT
              report describes a mathematical model that predicts the percent of
 the population affected by a pathogen or toxic substance emitted in a cooling tower
 plume, and gives specific applications of the model. Eighty-five pathogens  (or dis-
 eases) are cataloged as  potentially occurring in U.S. waters, but there is insuffi-
 cient data to predict the probability of occurrence or relate their occurrence to
 public health, population, or pollution. Sixty-five toxic substances are cataloged as
 potentially occurring in  U.S.  waters, but the actual number is probably many times
 the EPA-supplied list. Toxic concentrations to persons,  animals, and plants are
 known for only a few of the chemicals: most toxic levels can be only inferred from
 animal studies. In the population as a whole, the epidemiological impact of a patho-
 gen is a function of age,  sex distribution, racial (genetic) distribution, general
 health and well-being, prior exposure, and immunological deficiency states. While
 cooling device  drift may r.jt be directly responsible for epidemics, it may potentiate
 the burden in an already weakened population, raising a segment of the population
 into the clinical state. The effect of toxic substances is difficult to evaluate because
 of inadequate data on humans. The effect is a function of  concentration in susceptible
 tissue, and is much less dependent than pathogens on host resistance.
                             KEY WORDS AND DOCUMENT ANALYSIS
                 DESCRIPTORS
                                          b.IDENTIFIERS/OPEN ENDED TERMS
                                              c. COSATI Field/Group
 Pollution
 Cooling Towers
 Drift
 Plumes
 Pathology
 Toxic ity
Water
Epidemiology
Mathematical Models
Pollution Control
Stationary Sources
13B
13A,07A
14 B
2 IB
06E
06T
07B

12A
 3. DISTRIBUTION STATEMENT
 Release to Public
                                          19. SECURITY CLASS (This Report)
                                           Unclassified
                                              21. NO. OF PAOTS
                                                 215
                     20. SECURITY CLASS (This page)
                     Unclassified
                                              22. PRICE
EPA Form 2220-1 (9-73)
                                         208

-------
C
C"
                                                                                 Z
                                                                                 m
                                                                                 O
                                                                                 C
                                                                                 >

                                                                                 o
                                                                                    "0
                                                                                    rn
                                                                                    Z

                                                                                    r

                                                                                    "*-  -n
                                                                                    -n  -n
                                                                                 2  V  r
                                                                                 H  5  CD
                                                                                 C  <  C
                                                                                 z  i>  en

                                                                                 3^i
                                                                                 <  C  "
                                                                                 m  h  V
                                                                                 t)
                                                                                 r  0)
                                                                                 O  u
                                                                                 -<*  O
                                                                                 rn  °
                                                                                              O
                                                                                              U1
           C
    O     W
    (B
    2-  O m
    5   5 |
    -h  n  5:
    O   fD  "D
O  m

II
                                                                                                  o
                                                                                                  3
                                                                                                  3
    If  ^
    —  Q)
    30  3
    (5  Q.
                                                                                                    o
    o *c  __4
    3" CD  —
           >
           o
           m
           z
           o
o
o
~
Z
o
m
13
O
o
                 •--,  §.

                 <  ?
Xl

vj


f,)
O1
                 T3


                 ?
                                                                                                  c
                                                                                                  en
                                                                                                  m
                                                                                                  Z
                                                                                                 o  o

-------