oEPA
           United States
           Environmental Protection
           Agency
                 Region 3
                 Philadelphia, PA 19103
EPA/903/R-00/015
August 2000
www.epa.gov
Mid-Atlantic Highlands Streams
Assessment

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      The Mid-Atlantic Highlands study region includes the area from the Blue Ridge Mountains in
      the east to the Ohio River in the west and from the Catskill Mountains in the north to Virginia in
      the south.
Cover Photo by: Alan Herlihy

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                                EPA-903-R-00-015
                                    August 2000
   Mid-Atlantic Highlands
   Streams Assessment
                   by

Environmental Monitoring and Assessment Program
    National Health and Environmental Effects
            Research Laboratory
          Western Ecology Division
      Office of Research and Development
            200 S.W. 35th Street
           Corvallis, OR 97333-4902
                   &
                Region III
              1650 Arch Street
          Philadelphia, PA 19103-2029
     U.S. Environmental Protection Agency
              Washington, D.C.
               Final Report

                              Printed on recycled paper

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                                Notice
The information in this report was funded in part by the United States Environmental
Protection Agency (Environmental Monitoring and Assessment Program, Office of Research
and Development) through the Western Ecology Division. This report was subject to EPA's
peer and administrative review, and has received approval for publication as an EPA
document. Mention of trade names or commercial products does not constitute endorsement
or recommendation for use.

The suggested citation for this report is EPA 2000. Mid-Atlantic Highlands Streams
Assessment. EPA/903/R-00/015. US Environmental Protection Agency Region 3.
Philadelphia, PA.
                             Abstract
This report assesses the ecological condition of streams in the Mid-Atlantic Highlands and
ranks the potential stressors affecting stream condition. This study used an innovative
statistical survey, like a political poll, to sample almost 500 stream reaches throughout the
Highlands. The report defines stream condition in terms of the health of the biological
organisms in the stream, rather than just focusing on chemicals in the streams. The study,
however, also measured stream chemistry as well as the physical habitat in which these
organisms live. It found that a greater number of stream miles had biological organisms in
poor condition than in good condition throughout the Highlands. Overall, 31 % of the stream
miles were in poor condition based on a fish Index of Biotic Integrity and 27% were in poor
condition based on an aquatic insect index. Only 17% of the stream miles were in good
condition based on the fish Index of Biotic Integrity while 25% were in good condition based
on the aquatic insect index. For the first time, we have a benchmark for stream condition
across the Highlands and a scorecard against which we can compare future changes in stream
condition.

Key Words:  assessment, stream, fish, aquatic insect, scorecard, management,
            Region 3, stressors, stream condition, biotic index, watershed, ecoregion.
  Mid-Atlantic Highlands Streams Assessment

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                             Foreword
Water - the blood of life! If water is the
blood of life, then streams are the arteries
carrying that life-giving and life-sustaining
fluid from the uplands to the estuaries.
Streams have always been an integral part of
our society. Streams were the highways for
the western expansion in the early history of
this country. From rafts and river boats
moving people, goods, and materials to
markets along the Susquehanna, Allegheny,
and Ohio Rivers and their tributaries to
providing life sustaining water for drinking
and fish and wildlife, streams have sustained
the life-blood of the Mid-Atlantic region and
the nation.

Streams remain an important part of society
today - for recreation, for navigation, for
water supply, for peaceful, tranquil settings
that provide a respite from the daily grind.
The health of our streams is the responsibility
of all our citizens, but to protect and manage
these valuable resources, we need, first,
to know their current condition. The
innovative research study that is presented in
this report provides us with that knowledge.
Unfortunately, it is not good news. We have
come a long way in controlling pollution and
damage to our streams, but we still have
a long way to go. We can make those
improvements through programs like this that
help us understand what the condition of our
streams is, where the problems are, and
what factors are contributing to those
problems.

Unlike many previous studies that focused
only on chemical pollutants, this study used a
unique survey approach that emphasized the
biological condition of streams. This project
is the result of the joint efforts of many
individuals and organizations and represents
the way regional studies will be conducted in
the future. The US Environmental Protection
Agency Region 3 worked in conjunction
with the states of Delaware, Maryland,
Pennsylvania, Virginia, and West Virginia, the
EPA Office of Research and Development,
the US Fish and Wildlife Service, local
universities, and private contractors to design,
collect, analyze, interpret and present this
information. It is through the collective efforts
of all these organizations and individuals that
this innovative program was accomplished.

We now have a baseline of the condition of
Mid-Atlantic Highland streams we can use to
chart our progress in the future. We have a
scorecard of current conditions and a way to
generate future report cards on the condition of
Highland streams. Through the concerted
effort of all of us, we will continue to improve
the health of our streams so that all our citizens
can benefit and enjoy this unique resource.
                                             Mid-Atlantic Highlands Streams Assessment

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                 Acknowledgements
This report was prepared by U.S. Environmental Protection Agency Region 3 Mid-Atlantic Integrated
Assessment (MAIA) Team and the Office of Research and Development National Health and Ecological
Effects Research Laboratory Western Ecology Division.
The following individuals contributed to the Highland Streams Assessment Project and this report:

                     List of Contributors
EPA Region HI
DeMoss, Tom
Donaghy, Bob
Eaton, Richard
Green, Jim
Kanetsky, Chuck
Lorentz, David
Passmore, Maggie
Petrone, Frank
Preston, Ron
Rose, Carole
Walbeck, Eric

EPA-NHEERIVAED
Bradley, Pat
Pheiffer, Tom

EPA-NHEERIVWED
Kaufmann, Phil
Larsen, Phil
Olsen, Tony
Omernik, Jim
Paulsen, Steve
Peck, Dave
Stoddard, John

EPA-NERL/EERD
Hill, Brian
Klemm, Don
Lazorchak, Jim
Lewis, Phil
McCormick, Frank

EPA-NERL/ESD
Kutz,Rick

EPA-OEI
Davis, Wayne

OAOCorp.
Burch Johnson, Colleen
Cappaert, Marlys
OAOCorp.
Cassell, Dave
Comeleo, Pam
Hjort, Randy
Pierson, Sue
Rosenbaum, Barb

Oregon State University
Herlihy, Alan
Urquhart, Scott

Dvnamac Corp.
Bryce, Sandy
Hughes, Bob
Woods, Alan

Seniors in Environmental
Employment
Hails, Marge

TAICorp.
Brewer, Sandy
Herrin, Lori
Kneipp, Ann
McMullen, Dennis
Scopel, Darren
Smith, Mark
Wilts, John
Yeardley, Roger

USFWS
Drummond, Mike
Jenkins, Felton
McGowan, Peter
Pitt, Leslie

Delaware
Maxted, John

Maryland
Bo ward, Dan
Kazyak, Paul
Maryland
Klauda, Ron
Primrose, Niles
Rule, Tim

Pennsylvania
Bogar, Dan
Harris, Milt
Hepp, Joe
Kelly, Kein
Kime, Rod
Shaw, Tony
Shertzer, Rick

Virginia
Bolganio, Ralph
Cumbow, Ed
Gregory, Jean
Kain, Don
Seivard, Lou
Willis, Larry

West Virginia
Arcuri, Mike
Bailey, Jeff
Boggs, Dane
Scott, Mark
Smithson, Janice

FTN Associates. Ltd.
Benton, Jon
Frank, Bernadette
Remington, Robyn
Thornton, Kent

Science Applications
International Corp.
Scott, John

Tetra Tech. Inc.
Gerritsen, Jeroen
Jessup, Ben
  Mid-Atlantic Highlands Streams Assessment

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                Executive Summary
The purpose of this report is to assess the
ecological condition of streams in the
Mid-Atlantic Highlands and to identify and
rank stressors that might be affecting stream
condition. The first step in managing the
stream resources is to determine their current
condition.

To provide this information, an innovative
research, monitoring and assessment program
was initiated. This program, called the
Environmental Monitoring and Assessment
Program (EMAP), used a unique statistical
survey, like a political survey poll, to sample
almost 500 stream reaches across the Mid-
Atlantic Highlands during 1993 and 1994.
This sampling occurred in partnership with the
Mid-Atlantic States, the US Environmental
Protection Agency Region 3, the EPA Office
of Research and Development, the US Fish
and Wildlife Service, multiple universities, and
private contractors One innovative feature
of EMAP was that it used the health of
biological organisms living in these streams to
define the condition of the streams. It also
sampled the physical habitat in which these
organisms lived and the chemical quality of
the water. This permitted an assessment of
the condition of the Mid- Atlantic Highland
streams and a ranking of the stressors or
factors that were potentially affecting this
condition. For the first time, we have a
benchmark of stream condition across the
Highlands and a scorecard against which we
can compare future changes in condition.

STREAM CONDITION

What was the condition of the Highland
streams? In general, the results of this
assessment were not good news. Many
streams throughout the Highlands were in
poor condition. Over 31 % of the stream
miles in the Highlands were in poor condition
based on a fish Index of Biotic Integrity and
27% of the stream miles in the Highlands
were in poor condition based on aquatic
insect indicators. Only 17% of the Highland
stream miles were in good condition based
on the fish Index and 25% were in good
condition based on aquatic insects.  More
stream miles were in poor condition than in
good condition.

FACTORS AFFECTING CONDITION

What factors might be contributing to these
problems? The major stressor throughout
the Highlands is habitat destruction. Urban
sprawl and land use change are altering the
landscape throughout the Mid-Atlantic
region. Habitat destruction is occurring both
in the stream and along the stream banks,
removing trees and shrubs that provide
cover for fish and other aquatic organisms.
Some of the chronic problems that have
existed for decades such as mine drainage
and acid rain still persist. However, high
nitrogen and phosphorus concentrations,
which are major problems in other areas
of the country, are not apparent in most
Highland streams.

DIFFERENT PERSPECTIVES

Stream conditions do vary throughout the
Highlands, however, and it is useful to look
at stream condition from several different
perspectives such as ecoregions, water-
sheds, or at the state level. These different
perspectives help us see and understand
what is and what is not working and why.
                                           Mid-Atlantic Highlands Streams Assessment

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In the North-Central Appalachian ecoregion,
for example, 43% of the stream miles were in
poor condition based on fish indicators. If a
watershed perspective were used, 41 % of the
stream miles in the Kanawha-Upper Ohio
would be found in poor condition based on fish
indicators. Atthe state level, West Virginiahad
44% of their stream miles in poor condition
based on fish indicators. Habitat destruction
was still a major stressor in streams, regardless
of the geographic perspective. Other stressors,
however, varied depending on the geographic
boundary. For example, mine drainage
resulted in poor quality in 24% of the
North-Central Appalachian ecoregion stream
miles, but less than 1 % of the stream miles in
the Valley ecoregion had poor quality based on
mine drainage. Although nutrients were not a
problem throughout the Highlands, 20% of the
stream miles in the WesternAppalachian
ecoregion didhave high nitrogen concentrations.

A SCORECARD

A scorecard was developed for the
condition of streams throughout the region,
based on the different geographic areas and
management perspectives. This scorecard
provides a summary of stream condition and
stressors and can be used to target areas for
different protection, management, and
restoration programs.
MANAGEMENT IMPLICATIONS

Some of the implications for protecting,
managing and restoring streams in the
Highland region included:
       You can't play the game without a
       scorecard.
       You can't develop a scorecard using
       existing monitoring networks.
       A single indicator only tells part of
       the story.
       Chemical indicators don't tell the
       whole story.
       Just one management perspective is
       not enough.
       You can't evaluate the success
       of management actions without
       repeated monitoring.
THE FUTURE

We now have a baseline of the condition of
Mid-Atlantic Highland streams that can be
used to chart our progress for the future.
The  scorecard can tell us where we need
greater management attention for streams in
poor condition as well as better protection
for streams currently in good condition.
Through the concerted efforts of us all, we
can become good stewards of our stream
resources and leave a legacy for future
generations to enjoy.
  Mid-Atlantic Highlands Streams Assessment

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             Table of Contents


FOREWORD	iii

ACKNOWLEDGEMENTS	iv

EXECUTIVE SUMMARY	v

INTRODUCTION	1
  PURPOSE	1
  BACKGROUND	1
  STREAM CONDITION	2
  REGIONAL STATISTICAL SURVEYS	5
  THE HIGHLAND STREAM POPULATION	6

ECOLOGICAL CONDITION OF STREAMS	9
  FISH ASSEMBLAGES	10
  AQUATIC INSECT ASSEMBLAGES	12
  COMPARISON OF FISH AND AQUATIC INSECT SCORES	13

STRESSORS	15
  ACIDIFICATION OF STREAMS	16
  NUTRIENT RUNOFF	18
  PHYSICAL HABITAT ALTERATION	20
  FISH TISSUE CONTAMINATION	22
  WATERSHED DISTURBANCE	24
  NON-NATIVE FISH: STRESSOR OR SUCCESS STORY	25
  SUMMARY RANKING OF STRESSORS	26

ECOREGIONS, WATERSHEDS, AND STATES: ANOTHER
PERSPECTIVE ON STREAM CONDITION	29
  ECOREGIONS	29
  WATERSHEDS	36
  STATES	40
                             Mid-Atlantic Highlands Streams Assessment
I  vii

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         Table of Contents (cont)
  DEVELOPING A SCORECARD: SUMMARIZING STREAM
  CONDITION	45

  MANAGEMENT IMPLICATIONS	49

  WHERE DO WE GO FROM HERE?	51
            List of Appendices
  Appendix A - Additional Readings	53
  Appendix B - Stream Population Estimates	57
  Appendix C - Glossary	61
viii | Mid-Atlantic Highlands Streams Assessment

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                            INTRODUCTION
PURPOSE
The purpose of this report is to:

   1)   Assess and report on the ecological
       condition of streams in the
       Mid-Atlantic Highlands (the Highlands).

   2)   Identify and rank the relative
       importance of stressors* affecting
       stream condition.

The Mid-Atlantic Integrated Assessment
(MAIA) is an interagency, multidisciplinary
research, monitoring, and assessment
program to develop high-quality scientific
information on the region's natural resources,
current condition, stressors, trends, and
vulnerabilities. MAIA results and information
are intended to satisfy a broad group of
stakeholders' needs, convey important
information relevant to their assessment
questions and issues, and be useful in making
management decisions. Assessing the
condition of the Mid-Atlantic Highland
streams was a critical MAIA
project. Information in this       	
report is based on the
scientific data, analyses, and
results documented in the
literature cited in Appendix A.
BACKGROUND
The Mid-Atlantic Highlands
encompass approximately
79,000 square miles and extend from the Blue
Ridge Mountains in Virginia in the east to the
Ohio River in the west, and from the Catskill
Mountains in the north to the North Carolina-
Tennessee-Virginia state borders in the south
    This report
assesses Highland
 stream  condition
    and ranks
     stressors.
(see inside front cover). West Virginia is the only
state entirely within the Mid-Atlantic Highlands.
The Highlands contain many unique natural
features that combine to form a complex,
interconnected mosaic of terrestrial and aquatic
ecosystems. Streams run through forests
interspersed with wetland, residential, and
agricultural areas and integrate contributions
from all types of land use and cover. The
Highland landscape contains diverse hard-
wood forests, as well as many rare, threatened
or endangered plant and animal species. The
Shenandoah National Park, which lies within the
Ridge and Valley Province, is world renowned
for its beauty and variety of animal and plant life.

In addition to providing a home for a unique
variety of plants and animals, the Highlands are
also home to approximately 11.5 million people.
Residents of the Highlands can leave the hustle
and bustle of an inner city business at the end of
the work day and enj oy rural settings less than
an hour away. They can find tranquility while
fishing a Highland stream or hiking a mountain
trail. A system of roads and interstate highways
              (see inside front cover) allows
              them to enjoy the best of both
              worlds: natural settings and
              vibrant, active cities.
               Human desires for nature and
               civilization can be at odds with
               one another. The same roads
               and highways that provide a
               bridge between the two
worlds pose a potential threat to the ecosystems
surrounding them: road construction contributes
to soil erosion and the siltation of streams
(Figure 1); vehicular traffic contributes to air
pollution and adds contaminants to the highways
*Terms in the glossary are bolded at first usage.
                                             Mid-Atlantic Highlands Streams Assessment

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Figure 1. Road construction can contribute suspended
sediment to streams during and following rain storms.
that subsequently wash into streams during
storms. We depend on the electrical energy
provided by coal-fired boilers, as well as the
use of agriculture fertilizers and chemicals to
increase the production of food and fiber for
our nourishment and survival. Some of the
ecological effects associated with these
human activities include the accumulation of
trash in streams, drainage from mines and
mine tailings, nutrient runoff, and stream
channelization (Figure 2).

We are faced with a daily dilemma: we
want ready access to the conveniences of
modern society, and we want to sustain
environmental quality because it provides
links to our cultural past and inner peace.
People with different, sometimes opposing,
perspectives are asking, "How do we
provide goods and services to society in an
environmentally sound manner?" The first
step toward deciding this is to simply, but
objectively and rigorously, assess the current
status of our ecological resources - where
are we right now? With this perspective and
         baseline, our social and political
         institutions can assess where we
         are, where we want to be in the
         future, and what actions are needed
         to move us in that direction. An
         assessment of the ecological
         condition of streams in the
         Mid-Atlantic Highlands is one of
         the first steps in this process.

         STREAM CONDITION
         Most historic assessments of stream
         quality have focused on describing
         the chemical quality of streams and,
         occasionally, on sport fisheries
impacts. As we have made progress in
controlling chemical problems it has become
obvious that the ultimate concern is actually the
health of the plants and animals that inhabit
these streams and rivers.

In this assessment we have tried to address this
concern not by ignoring physical and chemical
measurements, but by shifting the focus to
direct measurements of the biota themselves.
In this assessment, the ecological condition of
                                   t *•
INDICATORS:
• An indicator is a sign that relays a
  complex message in a simplified and use
  ful manner.
• An ecological indicator is a measure
  that describes the condition of an
  ecosystem or one of its critical
  components.

Example — The presence of trout in a stream
indicates cool, well-oxygenated water, with lots of
aquatic life; therefore, the presence or absence of
trout is an indicator of stream condition.
  Mid-Atlantic Highlands Streams Assessment

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Figure 2. Unauthorized dumping, mine drainage, logging,
management practices, and similar human activities can lead
to degraded stream quality.
streams is defined by biological indicators.
The biological organisms in a stream integrate
the many physical and chemical stressors
and factors, including other biota
(parasites, predators, or   i	
competitors), that are
acting in, and on, the
stream ecosystem. Stream
condition can be determined
by assessing appropriate
biological indicators (Table 1),
or combinations ofthese
indicators, called indices.
Informationonthe ecologicalconditionof
streams is supplemented by measurements of
other stream characteristics, especiallythose
physical, chemical, or other biological factors
that might influence or affect stream condition.
These stream characteristics allow us to assess
the stressors of stream condition, based on
expected signals from maj or environmental
perturbations (e.g., habitat modification, mine
Historic assessments of
  stream quality were
     limited and too
  narrowly focused on
       chemicals.
            drainage, acid rain, agricultural
            nutrients, etc.). The combination
            of ecological and stressor
            indicators listed in Table 1
            represents our best current
            understanding of the biological,
            physical and chemical factors that
            collectively determine stream
            quality.

            Working in partnership with the
            states (Delaware, Maryland,
            Pennsylvania, Virginia, and West
            Virginia), the U.S. Fish and
            Wildlife Service (USFWS),
            U.S. Geological Survey
            (USGS), multiple universities,
and Environmental ProtectionAgency (EPA)
Region in, the EPAEnvironmental Monitoring
and Assessment Program (EMAP) assembled
crews in 1993 and 1994 to collect samples on
448 first- through third-order streams (see
                 pp. 6-7 for definition)
                 across the Mid-Atlantic
                 Ffighlands. All of the
                 crews had been trained to
                 use identical sampling
                 methods, so that com-
                 parisons across the region
                 could be made.
                 Perhaps the most unique aspect of this
                 assessment is that it uses data from a regional
                 statistical survey of streams to describe the
                 condition and characteristics of the entire
                 population of first - through third - order
                 streams in the Mid-Atlantic Ffighlands. It is
                 intended to answer, as directly as possible, the
                 question, "What is the condition of Ffighland
                 streams?"
                                              Mid-Atlantic Highlands Streams Assessment

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                                        Table 1
                Examples of ecological indicators measured in Highland streams.
Indicators of Condition                                 Purpose
Fish                         Important indicators of stream condition; middle to upper end of
                             food web; accumulate contaminants that are then consumed by
                             humans, other mammals, and birds. Caution: Some smaller streams
                             may naturally not have fish. No fish in small streams does not
                             automatically mean there are problems.
Aquatic insects
Indicators of stream condition and sensitive to environmental factors
such as pollutants, pH, and loss of algae. Insect populations can
recover rapidly when conditions improve.
Indicators of Stress
Water chemistry
                          Purpose
Chemical criteria and standards established for envionmental and
human health; nutrients and contaminants affect aquatic insects and
fish
Stream channel sedimentation   Sedimentation can smother algae and plants, aquatic insects, and fish
                             feeding and spawning areas.
Riparian habitat
Fish tissue contaminants
Stream bank alteration (removal of trees, shrubs, grasses, change of
grade) affects channel habitat structure, aquatic insects, and fish

Contaminants accumulate in fish tissue, and can adversely affect
humans and wildlife
Watershed condition
Different land uses can adversely affect stream biology, chemistry,
and physical habitat
                           In this assessment, stream
                           condition is defined by the
                        health of the living organisms.
   Mid-Atlantic Highlands Streams Assessment

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REGIONAL STATISTICAL
SURVEYS
In the past, EPA and the states addressed
municipal and industrial point sources of
chemicals as major threats to streams and
rivers. This led to focusing monitoring,
assessments, and controls very locally on
individual segments of streams above and
belowpoint source discharges. Monitoring
locations were selected to evaluate the
effectiveness of improved treatment of these
municipal and industrial discharges. As these
point sources were cleaned up, it became
apparent that a wider range of stressors also
was threatening our aquatic resources. Some
attempts were made to combine existing data
and use them in regional assessments, but the
limitations of this approach became apparent
   J_
   EPA's EMAP develops indicators
   and other research tools to track
 status and trends in the condition of
   the nation's ecological resources.
   These resources include estuaries,
 wetlands, inland lakes and streams,
    forests, and mixed landscapes.
 Figure 3. Statistically selected stream sites permit
 objective estimates of stream quality. About 450
 stream reaches were sampled in the Highlands
 during 1993 and 1994.
because the local sites were not representative
of other streams or areas in the region.
Another approach was needed to assess
stream quality on a regional basis.

EPA and the states in the Highlands wrestled
with this problem and came up with a different
approach for stream monitoring. In addition to
implementing direct measures of the ecological
    condition of the biota themselves,
    they devised away to pick monitoring
    locations that do not focus on known
    problem areas (e.g., sewage outfalls).
    Instead, monitoring sites were chosen
    through a statistical approach that provides
    a clear and obj ective view of the condition
    of all streams. It is hoped that this
    approach, and this assessment, can serve
    as models for future National Water
    Quality Inventories: A Report to Congress
    (also known as the 305 [b] Reports, after
    the section of the Clean Water Act that
    mandated the reports).

    To describe the condition of all streams
    within the Highlands, without sampling all
    of them, EMAP worked with EPA
    Region in and the states to develop a
    regional statistical survey of streams, with
    the goal of providing statistically unbiased
    estimates of stream condition throughout
                                              Mid-Atlantic Highlands Streams Assessment

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the Highlands (Figure 3). With this approach,
we can describe the condition of the streams,
the proportion of stream miles that are impaired
or degraded biologically, and characterize the
relative importance of stressors, such as mine
drainage or stream sedimentation.

A statistical survey of streams operates in the
same manner as the public opinion polls used
to project winners and losers of political
campaigns. A subsample of stream reaches
is selected at random to represent the
population of streams in a region, j ust as the
subsample of individuals in apublic opinion poll
is selected to represent the voting population as
a whole. Regional statistical surveys have been
used for many years in forestry and agricultural
monitoring programs to determine the condition
of forests and agricultural lands, but their use in
assessments of aquatic ecosystems is just
beginning. Additional information on the
EMAP stream design can be found in the
references listed in Appendix A.
THE HIGHLAND STREAM
POPULATION
Historically, management practices have
focused on large streams, which are best
known to the public due to their use in
navigation and boating, and their visibility
from major road crossings. Small streams,
on the other hand, dominate the total stream
length in the region, contribute to the quality
and condition of larger streams and rivers,
and are critical to determining the condition
of all Highland streams and rivers.

Small, first-order streams are the dominant
stream class in the Highlands; over
51,000 stream miles (i.e., 63% of the total
length) are classified as first-order streams
(Figure 4). Second-order streams are larger
and start at the point where two first-order
streams join. Over 12,000 stream miles in the
Highlands (i.e., 15%) are in second-order
streams. Third-order streams consist of two
 Figure 4. The majority of streams in the Mid-Atlantic Highlands (i.e., 89% or 72,200 stream
 miles) are classified as first- through third-order streams. This stream classification is illustrated
 above for one hypothetical watershed in the Highlands. The confluence (joining) of two first
 order streams forms a second order stream; the confluence of two second order streams
 forms a third order stream, etc. (See Strahler 1956 for more information.)
  Mid-Atlantic Highlands Streams Assessment

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o
S
Irt
   (or more) second-order streams coming
   together and about 8,850 stream miles in
   the Highlands (i.e., 11%) are in third-order
   streams. All higher-order streams
   (i.e., fourth-order and higher) constitute
   only 11% or 8,000 stream miles.

   The size or order of a stream not only
   affects its natural characteristics, but also its
   capacity to handle both point source and
   nonpoint source pollutants. Stream size
   frequently affects the size and type of biotic
   community present, particularly for fish, and
   may control the relative importance of
   factors to which the biota respond. Very
   small streams (first-order, headwater
   streams) are often quite clear and shaded
   by trees; they are likely to be dominated by
                    Leaves, Debris, Clear Water,
                    Canopy Covered, Small Fish, if
                    any fish
                                                   I
                   Small streams  are an
                    important Highland
                    resource. 63% of the
                    stream miles are in
                    headwater streams.
             aquatic insects in the stream bottom and
             with small fish that feed on these bottom
             organisms. Large streams (sixth- to
             seventh-order rivers) are often muddy with
             canopy cover only along the banks and are
             dominated by larger fish that feed along the
             shoreline. While streams larger than third
             order are not covered in this assessment,
             this continuum in stream size and
             characteristics is an important controller of
                        what we expect to find in
                        streams of different sizes
                        (Figure 5).
Aquatic Plants, Surface
Exposed to Sunlight,
Game Fish
                                        Turbid, Floating
                                        Algae, Larger Fish,
                                        Navigation,
                                        Recreation
 Figure 5. Streams change their characteristics as their size
 or order increases.  Smaller first- to third-order streams
 dominate in the Highlands. Their quality is critical to sustaining
 the quality of larger rivers.
The stream network used for
selecting sampling sites in this
assessment, and for estimating
the total length of streams in the
region, was the EPA River
Reach File, Version 3. This
digital database includes all
streams that are represented on
USGS maps at a scale of
1:100,000. The map scale
used is important because it can
affect the estimate of stream
miles. The stream network
shown on 1:100,000 scale
maps was considered a good
index of the population of
Mid-Atlantic streams.
                                                Mid-Atlantic Highlands Streams Assessment

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Mid-Atlantic Highlands Streams Assessment

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       ECOLOGICAL CONDITION OF  STREAMS
To assess the overall condition of Highlands
streams, we looked at multiple biological,
chemical, and physical indicators. To answer
the specific question "What is the ecological
condition of Mid-Atlantic Highland
Streams? "we rely on direct measures of the
biological communities that inhabitthe
streams. Throughout this report, ecological
condition - good, fair, or poor - is determined
     Biological organisms
       integrate all of the
   stressors to which they are
     exposed.  Their health
   defines stream  condition.
by biological indicator or index scores. The
fish, aquatic insects, and other animals and
plants in a stream serve as "integrators" of all
the stressors to which they are exposed. The
fish and insects respond to the cumulative
effects of chemical contaminants, modification
of their physical habitat, and changes in both
the amount and the timing of the flow of
water. Historically, sport fish (e.g., trout,
smallmouth bass) have been the primary biotic
component of interest to the public, and an
emphasis has been placed on the condition of
sport fisheries in larger rivers. This emphasis
on sport fish and large rivers has resulted in a
narrow, incomplete view of the status of
Highland streams, where large rivers make up
only about 10% of the total stream length.
Some people have defended this large river/
sport fish perspective by claiming that small
streams do not support fish. On the contrary,
  we find that headwater streams can be very
  important in providing suitable habitat for both
  fish (e.g., minnows, chubs) and sport fish (e.g.,
  brook trout, smallmouth bass) (Figure 6). One
  interesting exercise to put this number in
  perspective is to assume that all fourth - order
  and larger streams have sport fish present.
  If so, then second -, third-, and fourth and larger
  - order streams all have approximately the same
  total length with sport fish present (8,100,8,200,
  and 8,900 miles, respectively). We estimate first-
  order streams have nearly twice those lengths
  (i.e., 14,300 miles) with sportfish.

  By sampling both fish and aquatic insect
  assemblages throughout the Highlands, we
  have the opportunity to move beyond a
  narrow, sport fisheries focus, and look instead
  at the biological integrity of stream ecosystems.
  Biotic integrity has been defined as, "the
  capacity of an ecosystem to support and
  maintain a biota that is comparable to that
  found in natural conditions." Most people
  100
O)
CD 60-

E
CO
g) 40-
I I Sport fish
I I Fish, but no spc
I I No fish caught



1
rtfi

sh
— I

n



— 1
 1st Order
(42,830 miles)
2nd Order
(13,770 miles)
                                 3rd Order
                                 (7,800 miles)
Figure 6. Fish distribution in 90% of Highland
streams (i.e., first through third order), by stream
order. Even first order streams have sport fish.
                                            Mid-Atlantic Highlands Streams Assessment

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would agree (as would the stipulations of the
Clean Water Act) that maintaining the biotic
integrity of streams is a worthy goal. This
assessment is one of the first steps toward
achieving that goal.

FISH ASSEMBLAGES
Streams must meet a number of requirements
if they are to support healthy fish assemblages -
providingasufficientvariety of foods, clean
bottom gravel for spawning, and a habitat
with diverse forms offish cover, among
others. In analyzing the Highlands fish data, a
series of indicators, or metrics, was used to
measure how well the stream is meeting these
requirements. Examples offish metrics are:
the number offish species present who
cannot tolerate pollution; the proportion of
individuals present that require clean gravel
for spawning; or the number of bottom vs
water column species present. Each metric
was scored against our expectations of what
value was possible for each stream (based
on reference conditions - see box), and then
combined to create an overall Index of
Biotic Integrity (IBI), whose values range
from 0 to 100.
                               Reference Condition

 In order to measure the biotic integrity of streams, we must rely on some estimate of the
 streams' reference condition. This is the minimally disturbed, or "natural", condition referred
 to in the definition of biotic integrity (see text). In order to understand how we approached
 estimating reference condition in MAHA, it's useful to employ an analogy with which we are
 all familiar. Suppose that you wanted to use human body temperature as an indicator of
 human health (as is commonly done). One of the first things you would need is information
 on the normal range of temperatures. In order to estimate this range, or distribution, you
 might draw a subsample of the human population that is validly 'healthy.' The range of
 temperatures measured in this subsample is an estimate of reference condition for this
 indicator. Next, we'd want to know how far away from this distribution (or how extreme) a
 temperature needs to be before we'd consider it to be unhealthy. In the case of body
 temperature, we might have very high confidence that we've correctly identified a healthy
 subpopulation, and the range of temperatures might be fairly small. In this case, we could use
 something like the ends or extreme values from the reference distribution (e.g. the lowest 5%
 or highest 5% of body temperatures measured from a large group of people), as thresholds
 beyond which we identify a temperature as unhealthy. We use a similar approach for the
 biological data we report in MAHA - identifying a healthy subsample of sites, collecting
 indicator information at each one, and describing a distribution of reference condition values
 - but have less confidence that all of the sites we identify as 'healthy' truly are. For this
 reason, we use more conservative thresholds than we used in the body temperature example.
 Commonly, the 25^ percentile value (of the reference distribution) is used as a threshold
 between sites in good condition, and those in fair, or marginal, condition. We also adopt the
 1 ** percentile as the threshold between sites in fair condition and those in poor condition. For
 these sites, we can be 99% confident that their biotic integrity values are below anything
 found in our subsample of sites in minimally disturbed, or natural, condition.
  Mid-Atlantic Highlands Streams Assessment

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   Using fish indicators, almost
     twice as many Highland
    stream miles were in poor
    condition  (31%) as  in good
          condition  (17%).
         on the development of the Highlands fish IBI,
         and the setting of thresholds, please see the
         references listed in Appendix A.

         In the Highlands as a whole, approximately 17%
         of the stream length is considered to be in good
         condition; that is, 17% of the Highlands stream
         length had IBI scores greater than 72 (Figure 7).
         36% ofthe streamlength was infair condition,
The definition ofbiotic integrity
described above introduces the
concept of "natural conditions" against
which each stream's biotic integrity
should be compared. Our best
description of natural conditions is
derived from measurements made at
reference sites. For MAHA, we
established a small collection of sites
that represent the best conditions that
are observable today, i.e., sites that are
free of influences from mine drainage,
nutrients, habitat degradation, etc. The
IBI scores calculated for these sites
range from 57 to 98; this range
describes a distribution, which we use
to estimate reference conditions for the
Highlands region (see box). The 25^
percentile of this distribution (72) is the
value we use to distinguish sites that
are in good condition from those in fair
condition. The 1 ** percentile value (57)
separates sites in fair condition from
those in poor condition. Another way
to describe (statistically) this setting of
thresholds is to say that we are 99%
certain that any value less than 57 is
below the range of values we see in
reference sites. For more information
  Highland Region
      Overall
Figure 7. Fish IBI scores in Highland streams showing the
proportion ofthe stream miles in good, fair, and poor condition.
Red, yellow and green markers on the map correspond to
individual sites in poor, fair or good condition. About 31% of
the Highland stream length has poor fish assemblages, relative
to those found in reference sites. Streams with 'no fish were
caught' were sampled, but most are too small for us to predict
reliably whether they should be expected to have fish. Within
the Highland region are subareas that represent management
areas such as ecoregions (shaded areas above), watersheds
and states. These are discussed in later sections.
                                              Mid-Atlantic Highlands Streams Assessment T  11

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   and 31% was in poor condition; that is, 31 %
   of the stream miles have IBI scores less than 57
   (Figure 7). About 16% of the Highlands
   stream length drains watersheds that are too
   small for us calculate an IBI reliably. Most of
   these streams had very few (or no) fish
   collected, and may be too small for us to
   expect to find healthy fish populations present.

   AQUATIC INSECT
   ASSEMBLAGES
   An additional picture of stream condition
   can be derived from examining the aquatic
   insects (and other bottom-dwelling
   invertebrates) in streams (Figure 8). These
   animals provide food for fish and other
   wildlife, and serve as a link between plants
   and higher levels of the food web. One
   aquatic insect index, EPT, has been used
   extensively to evaluate stream condition
   throughout the United States. It is calculated
   from the number of species that are found in
   three orders of aquatic insects - mayflies
   (Ephemeopterd), stoneflies (Plecoptera),
   and caddisflies (Trichopterd); the index gets
   its names from the first initials of these three
   orders (EPT). Many of the species in these
   three orders are sensitive to pollution and
   other stream disturbances, and the total
   number of species is a good gauge of how
   disturbed any given stream may be. EPT
   scores from least-disturbed Highland streams
   were used to set expectations (very analogous
   to the best attainable condition perspective for
   the fish IBI). Expectations were set separately
   for streams with fast-moving sections or
   "riffles" (the vast majority of Highlands streams)
   and slow-moving streams where "pools"
   dominate, because fewer EPT species naturally
 occur in pools. For riffles, 75% of the least-
 disturbed streams had at least 17 species of
 EPT present, so we used this as our definition
 for good condition. In pools, the corresponding
 number of EPT species was 6. The remainder
 of the least-disturbed streams had between 9
 and 16 species (riffles) or 3-5 species (pools),
 so we used these criteria to define fair condi-
 tion. All streams with fewer than 9 (riffles) or 3
 EPT species (pools) were classified as in poor
 condition.
Figure 8. Sampling aquatic insects during
May-July in Highland streams. Biological
organisms are used to define stream conditions.
12  | Mid-Atlantic Highlands Streams Assessment

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  Highland Region
      Overall
                                      <*«
   ^ s   °
•  V1 3oDo^l
a
-------
                                     Table!
 Comparison between fish index scores and aquatic insect scores for the condition of stream
 miles in the Mid-Atlantic Highlands.
                                           % Stream Miles in Poor Condition
                 Area
  Highlands Region
    Good
    Fair
    Poor
    Not Estimated
 Aquatic Insects

         25
         48
         27
         0
Fish Index

    17
    36
    31
    16
Because the fish and aquatic insect groups
respond differently to different stressors, it is
important that we do not rely on just one
index. We run the risk of missing some
problems if we use just one or the other.
Although there are differences, both indices
indicate over one quarter of all stream miles
in Highland streams are in poor condition.
Why?
                           Both fish and insect
                           indices indicate over
                            25% of Highland
                           stream miles are in
                             poor condition.
  Mid-Atlantic Highlands Streams Assessment

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                                STRESSORS
In the previous section, the ecological
condition of the streams in the Mid-Atlantic
Highlands was described based on direct
measurements of stream biota. Here we
present our findings on the stressors to the
streams of the Highlands region. These are
based on direct measures of physical,
chemical or biological characteristics of
streams and their watersheds. There are
stream attributes that can be directly or
indirectly altered as a result of human activity
or intervention in the stream system, and
that have been known to have harmful effects
on stream biota. They are described as
"potential" stressors because analyses to date
have examined only the extent and distribution
of these stressors. We have yet to establish
the statistical relationships between these
stressors and the biological conditions
described above. We present this information
in the belief that comparisons of stressors will
be useful to regional managers in determining
where best to focus their limited resources for
stream protection and restoration, when it is
warranted. Additional technical information on
the stressors and their measurement can be
found in the references listed in Appendix A.

The heterogeneous nature of the land use
and land cover in the Mid-Atlantic Highlands
is evident from satellite imagery (Figure 10).
Agricultural areas, urban and suburban
clusters, forests, mining sites, and other
features are interwoven into the landscape.
            • Urban
            [HI Agricultural
            • Rarest
            EH Water
            EH Barren
              Figure 10. Land use classified from satellite imagery showing
              the complex mosaic of ecological systems in the Highlands.
                                              Mid-Atlantic Highlands Streams Assessment T  15

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Human activities have the potential to alter
stream quality and affect the biota that lives
in these streams. The characteristics or
stressors in Highland streams and their
watersheds included in this report are:

    •   Stream acidification,
    •   Nutrient runoff,
    •   Habitat alteration,
    •   Fish tissue contamination,
    •   Watershed disturbance, and
    •   Non-native fish introductions.

A brief description of each stressor is
provided, followed by results. Where
standards for the individual stressors are
generally accepted, they were used to
separate streams (or watersheds) into
good, fair and poor quality classes. Where
such standards do not already exist, we
summarize the results using a scale that we
believe is a defensible interpretation of
information for that stressor. At the end of
this section, the stressors are compared
or ranked against one another so that the
reader can develop some appreciation for
differences and similarities in the extent
and distribution of these stressors. This
"comparative" or "relative" ranking simply
compares the length of stream resource in
poor quality for that stressor.

ACIDIFICATION OF STREAMS
Streams can become acidic through the effects
of acid deposition (deposition of nitrogen and
sulfur compounds produced by burning fossil
fuels) or when water percolates through mines
and mine tailings (mine drainage) (Figure 11).
The Highland region is unusual because it
receives some of the highest rates of acid rain in
the U.S., has geology that makes large areas
within the region susceptible to acidification,
and has a high incidence of coal mining.
Mountainous areas have shale and hard,
igneous rocks that don't neutralize acids very
well while some of the valleys have limestone,
which does neutralize acids (see box on acid
rain and mine drainage). Evaluations of stream
chemistry (e.g., acid neutralizing capacity
[ANC, in units of ueq/L], is a measure of the
stream's ability to neutralize acids and buffer
or prevent large pH changes) allow us to
determine if streams are acidic (ANC<0) and
whether the acidity is due to acid rain or to
mine drainage (see text box on next page).

Streams may be acidic throughout the year
(chronically acidic) or only for short periods
when flows are high such as during storm
events (episodically acidic). Because Highland
streams were sampled during spring base flow,
and not during storms, the data are best suited
to estimating chronic acidity. Across the
Highlands as a whole, less than 4% of the total
stream length was chronically acidic (ANC<0)
due to acid rain. How would this number
Figure 11. Both acid rain and mine drainage
can make a stream acidic, but mine drainage
can contribute sediment and metals that further
degrade streams downstream.
  Mid-Atlantic Highlands Streams Assessment

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                           Acid Rain vs Mine Drainage
What is acidity? The acidity of a substance
is measured using a pH scale that ranges
from 0 (very acidic) to 14 (very alkaline). A
value of 7 is considered neutral, and each
number on either side is logarithmically more
acidic or alkaline (for example, pH 6 is ten
times more acidic than a pH of 7, and pH 5
is one hundred times more acidic than pH 7).

"Normal rain", measured at pH 5.6, is now
rare for most of North America.  Today the
rain over the Mid-Atlantic is often over 100
times more acidic than "normal".

How does acidity effect stream biota?
At a 6.5 reading on the pH scale, large fish
begin to die. At 5.0 all zooplankton disappear,
at 3.0 all fish disappear, and at 2.0 all insects
disappear.

What is acid neutralizing capacity
(ANC)? ANC is a measure of the capacity
of dissolved constituents in the water to react
with and neutralize acids.  ANC is used as an
index of sensitivity of streams to acidification.
The higher the ANC, the more acid a stream
can assimilate before experiencing a decrease
in pH.  When ANC approaches zero, the
stream loses the capacity to buffer acid.

What is acid rain? Acid rain is rain that is
more acidic than normal.  The smoke and
fumes from burning fossil fuels rise into the
atmosphere and combine with the moisture in
the air to form acid rain. Acid rain usually
forms high in the clouds where sulfur dioxide
and nitrogen oxides react with water, oxygen,
and oxidants. This forms a mild solution of
sulfuric acid and nitric acid. Rainwater,
snow, fog, and other forms of precipitation
containing those mild solutions of sulfuric and
nitric acids fall to the earth as acid rain.
Acid rain may affect the soils, vegetation and
water throughout a watershed. Controlling
acid rain requires the regulation of sources
often hundreds of miles away from the
affected  stream.

What is Acid Mine Drainage?  Mine
drainage is metal-rich water formed from
chemical reaction between water and rocks
containing sulfur-bearing minerals. The runoff
formed is acidic and frequently comes from
areas where ore- or coal mining activities
have exposed rocks containing pyrite - a
sulfur bearing mineral.  This produces sulfuric
acid and releases a number of metals such as
iron, aluminum and manganese into streams.

Mines, especially when they occur near
streams, usually have drainage that goes
directly into the stream, contributing
sediment, toxic metals, and acids. Treating
mine drainage, often by constructing
catchment basins below the mines, must be
done at a local scale.
                                               Mid-Atlantic Highlands Streams Assessment T  17

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   change if we considered both chronic
   and episodic acidity? The National Acid
   Precipitation Assessment Program
   (NAPAP) concluded in 1990 that streams
   with ANC values lower than 50 ueq/L are
   susceptible to episodic acidification. These
   streams may experience fish kills and
   changes to their insect communities during
   short-term pukes of acid ramrunoff. When both
   chronic and episodic acidity are considered,
   about 11 % of the total stream length in the
   Highlands would be considered to be affected
   by acid rain (Figure 12).
Figure 12. Proportion of stream miles that are
acidic or susceptible to acid rain. Note some areas
are more susceptible to acid rain because of low
buffering capacity in the soils and bedrock.

   Streams that are acidic due to mine drainage
   are much less common in the Highlands
   than streams acidified by acid rain. But mine
   drainage effects extend far beyond acidification,
   including downstream sedimentationand toxic
   metal contamination (metals reside primarily in
   bottom sediments in non-acidic streams).
These less well-known stresses can have
pronounced effects on bottom-living organisms.
While about 1 % of stream miles in the
Highlands are acidic because of mine drainage,
14% of the stream length is non-acidic, but
degraded by mine drainage (Figure 13).
                                                Figure 13. Proportion of stream miles that are
                                                affected by mine drainage. Coal is found in some
                                                Appalachian areas.
NUTRIENT RUNOFF
The introduction of excessive nutrients into
streams can increase algal growth. If it
becomes extensive, algal growth can deplete
the oxygen in the water, choke out other
forms of biota, and significantly alter the
animal communities present. The increase
in nutrients typically can be seen as higher
concentrations of phosphorus, and the
dominant sources are usually municipal/
industrial discharges and runoff from
agricultural fields (Figure 14). In general,
phosphorus concentrations are low in
18  | Mid-Atlantic Highlands Streams Assessment

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      JI
         Chronic problems
        with acid rain  and
          mine drainage
           still persist in
        Highland streams.
Highland streams, with about 90% of the
stream length having concentrations less than
Figure 14. Nutrient enrichment to streams can
come from animal wastes such as livestock
(cattle,  hogs, and chickens); agricultural
fertilizer applications to fields; and municipal
and industrial waste treatment discharges.

50 parts per billion (ppb) (Figure 15). Only
5% of the stream miles have total phosphorus
concentrations that are considered high
(greater than the EPA guideline of 100 ppb).

Nitrogen is another nutrient that can stimulate
plant growth, especially in the presence of high
phosphorus concentrations. Like phosphorus,
nitrogen is commonly found in agricultural
fertilizers, but may also originate from acid rain
(nitrogen deposition) and sewage discharges.
There are no nitrogen guidelines for streams as
  Figure 15. Total Phosphorus concentrations
  in most Highland streams are low (<50 ppb)
  or moderate (50-100 ppb).
there are for phosphorus, although EPAis
currently developing new criteria for both
nutrients. However, the ratio between nitrogen
and phosphorus can serve as an indicator for
when aquatic plants grow near their optimum
rate. For the purposes of this assessment, we
set total nitrogen thresholds by multiplying the
total phosphorus thresholds by a nitrogen:
phosphorus ratio (15:l)typicalofundisturbed
sites. This gave us nitrogen thresholds of
750 (for the division between good and fair
condition) and 1,500 ppb (forthe division
between fair and poor condition).
      T_
         Stream  nutrient
      concentrations in the
      Highlands are in the
       good quality range
                                             Mid-Atlantic Highlands Streams Assessment T  19

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    Based on these total nitrogen thresholds,
    85% of the Highland stream miles were
    scored good, 10% fair, and 5% poor
    (Figure 16). Overall, nitrogen problems
    appear to be slightly greater in extent in the
    Highlands than do phosphorus problems, but
    the use of different nutrient criteria could
    alter this conclusion.
  Figure 16. Total Nitrogen criteria for Highland
  streams were based on a ratio between total
  nitrogen and total phosphorus. 85% of stream
  miles were scored good compared to only 5%
  scored poor for nitrogen.


    PHYSICAL HABITAT
    ALTERATION
    High quality physical habitat is an important
    and often overlooked ingredient for good
    stream condition. In the course of EMAP
    sampling, data were collected on many aspects
    of both riparian and instream habitat known to
    be important to biota. These data also can be
    used to diagnose the possible causes of habitat
degradation. In this assessment, we focus on
two characteristics of stream physical habitat
(riparian habitat and sedimentation) that play
perhaps the largest roles in establishing high
quality streams for both fish and insects.
Riparian (or streamside) vegetation shades
streams, particularly small streams, maintaining
cool water temperatures required by
many biological organisms for growth and
reproduction. It also strengthens and stabilizes
stream banks and helps to prevent silt and
associated contaminants from entering the
stream. Riparian vegetation that washes into the
stream can be a source of food for stream
organisms. Instream large woody debris
derived from riparian frees creates complex
habitat and pools for stream fish and aquatic
insects. Complex physical habitat within the
stream itself provides areas where fish and
aquatic insects can reproduce, feed, and hide
from predators. Human beings alter stream
physical habitat in a variety of ways: clearing
vegetation from the banks and riparian areas,
logging or farming up to the stream edge,
building roads across streams, dredging and
straightening the stream channel, and building
dams or other diversion structures in the
stream channel (Figure 17).

Habitat is the place or environment where a
plant or animal naturally or normally grows
and lives. Habitat combines food, water,
shelter, and nesting or nursery areas. All
plants and animals have specific habitat
requirements that must be satisfied in order
to live and thrive. Think of habitat as a "Life
Support System."

In general, good stream habitats have the
following basic characteristics: 1) wide,
naturally vegetated riparian areas, 2) mean-
20  | Mid-Atlantic Highlands Streams Assessment

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         Figure 17. Removing trees, shrubs and other tall grasses from stream banks
         contributes to poor riparian habitat.
dering channels, 3) a variety of substrate types
(such as wood, roots, and rocks), and 4) a
variety of water depths and water velocities.

The Index of Riparian Habitat Quality mea-
sures the condition of the riparian areas along
the banks of the stream (characteristic # 1,
above). The metrics in the Index are: area
covered by vegetation, types of vegetation
(canopy, mid-layer and ground layer as well as
coniferous and woody), and the intensity of
human-generated activities (logging, agricul-
ture, pipes, dams, etc.).

The Index of In-Stream Habitat measures the
condition of the stream itself
(characteristics #2-4, above).  Some of the
metrics in this Index are channel sinuosity
(meandering vs straightness), amount of various
types of substrates (sand, clay, rock, gravel,
bedrock, wood or detritus), and water depth
and velocity characteristics (dry channel,
pools, riffles, falls).

Results of this assessment indicate that habitat
degradation is a widespread problem in the
Mid-Atlantic Highlands. Management actions
that can be directed at this problem include
protecting existing riparian forests, replanting
protective vegetation along stream banks, and
restoring streams to more natural flow regimes.

We incorporated aspects of riparian vegetation
cover, riparian vegetation structural complexity,
and the intensity ofhuman disturbances into an
index of Riparian Habitat Quality for use in this
assessment. Based on historic literature and
the judgement of experts, we assumed that
                                                Mid-Atlantic Highlands Streams Assessment T  21

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Figure 18. Riparianhabitat quality forHighlandstreams.
24% of stream miles were in poor condition and 48%
were in good condition for riparian habitat quality.

    the pre-colonial reference condition for
    riparian vegetation in the Highlands is a
    multi-storied corridor of woody vegetation,
    with canopies that are closed (or nearly
    closed), and free of human disturbance. The
    resulting index varies from 0 to 1, with values
    less than 0.5 indicating streams with poor
               Almost 25% of
          Highland stream miles
             have poor riparian
             habitat and excess
          channel sedimentation.
    quality, values from 0.5 to 0.63 indicating
    streams with fair quality, and values greater
    than 0.63 suggesting streams with good
    riparian habitat.
In order to assess stream sedimentation, we
compared measurements of the amount
of fine sediments on the bottom of each
stream with expectations based on each
stream's ability to transport fine sediments
downstream (a function of the slope,
depth and complexity of the stream). When
the amount of fine sediments exceeds
expectations, it suggests that the supply of
sediments from the watershed to the stream is
greater than the stream can naturally process.
For the purpose of this assessment, streams
containing 90% or less of the predicted value
of fine sediments were rated good.  Those
streams where the fine sediments ranged from
90% to 120% of the predicted value were
rated fair, while those streams where fine
sediments exceeded 120% of the predicted
value were rated poor.

Physical habitat results for the Highlands as a
whole indicate that 24% of the total stream
length had poor riparian habitat, and 25%
of the regional stream length had excess
sedimentation (Figures 18 and 19). A higher
proportion of stream miles have riparian zones
with good habitat (48%) than have low
sedimentation (35%) (Figures 18 and 19).

FISH TISSUE CONTAMINATION
EPA has established criteria to protect
bothhuman beings and fish-eating wildlife from
chemical contaminants that can be concentrated
infish tissue. During EMAP sampling in the
Highlands, fish tissue samples were collected
and analyzed for selected organic and metal
contaminants. These contaminants included the
potentially cancer-causing organic contaminants
chlordane, dieldrin, heptachlor, and DDT, and
the toxic  metals arsenic and mercury. In the
 22  | Mid-Atlantic Highlands Streams Assessment

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Figure 19.25% of stream miles had poor quality
while 35% had good instream habitat based on
excess sedimentation.
analyses require a relatively large amount of
fish tissue, and not enough fish could be
caught in many streams to get sufficient
tissue.

For streams with sufficient fish tissue for
analysis, about 10% of the stream miles in
the Highlands had at least one  organic
contaminant that exceeded human health
criteria for carcinogens (Figure 20).

The only metal contaminant that exceeded
wildlife criteria for fish-consuming mammals
was mercury. Mercury concentrations in fish
tissue exceeded the mammalian wildlife
criteria in 4% of the Highland stream length
(Figure 21).
     case of the organic contaminants, fish tissue
     concentrations were compared to human
     health carcinogenic criteria. For the metals,
     we used mammalian wildlife protection
     criteria, which are lower than human health
     criteria. If fish tissue concentrations for any
     of the chemicals exceeded any of the criteria,
     the stream reach was classified as having
     contaminated fish tissue.

     For the Highlands, 44% of the stream miles
     did not have sufficient quantities offish tissue
     collected to do the analyses (Figure 20). The
           About 10% of stream
               miles had fish
              contaminated by
             organic chemicals.
 Figure 20. Fish tissue from about 10% of the
 stream miles in the Highlands had at least one
 organic contaminant that exceeded human health
 criteria. Note: No fish or insufficient fish tissue
 available for analysis occurred in 44% of the
 stream miles.
                                                 Mid-Atlantic Highlands Streams Assessment T 23

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                          • No Contaminants
                          • Contaminated Fish Tissue
                          O No Fish Data
  Figure 21. About 4% of the Highland stream
  miles had fish tissue mercury concentrations that
  exceeded mammalian wildlife criteria. Note that
  fish quantities were not sufficient for analysis in
  about 44% of the stream miles.

    WATERSHED DISTURBANCE
    With increased population growth, a more
    mobile population, and the economic pressures
    of development, the effects ofhuman activity
    also become more widespread. Streams reflect
    the quality of the watersheds they drain. To
    gauge the intensity of watershed disturbance in
    the Highlands, a watershed condition index
    was used to rank the disturbance in water-
    sheds upstream from the EMAP stream
    sampling locations. The condition rank ranged
    from 1 for watersheds that were minimally
    disturbed (low road density, limited or no
    agriculture, no buildings, etc.) to 5 for water-
    sheds that were heavily disturbed (crop
    production, urban development, mining, oil
    drilling, stream channelization, etc.). Forthe
    purposes of this assessment, we classified
    watersheds with condition scores of 1,2 or 3
    as minimally disturbed. Those with scores of 4
                                                we classified as moderately disturbed, while a
                                                score of 5 indicated a heavily disturbed
                                                watershed.

                                                Forthe entire Highlands, about 45% of
                                                stream miles were located in watersheds
                                                ranked in minimally disturbed condition,
                                                30% in watersheds ranked as moderately
                                                disturbed, and 25% in watersheds ranked in
                                                heavily disturbed condition (Figure 22).
      J_
         25% of Highland
       stream miles were in
         heavily disturbed
             watersheds.
The kinds of watershed disturbance that
contribute to watershed condition scores
also produce the "signals" that we associate
                          • Minimally Disturbed
                          O Moderately Disturbed
                          • Heavily Disturbed
Figure 22. Forthe Highlands, 45% of the stream
miles were in watersheds that were minimally
disturbed, 30% moderately disturbed, and 25%
were highly disturbed.
24  | Mid-Atlantic Highlands Streams Assessment

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with the other stressors discussed in this
section. A large number of mines, for example,
will lead to a poor watershed condition score,
and will produce the high sulfate concentrations
that we use as an index of mine drainage
effects. Because the watershed condition
classes are, in this sense, more cumulative
measures of stress than any of the other
indicators discussed in this section, we do not
include them in the relative stressor rankings
discussed below.

NON-NATIVE FISH: STRESSOR
OR SUCCESS  STORY?
"Great brown trout stream!" to some people
means a successful fisheries management
program. To others, it can mean the loss of
biotic integrity and a threat to native fish
species. In other words, some people
consider fish stocking of non-native species
to be a potential stressor in the stream.
However, many states have specifically
designated a stocked trout fishery as the
aquatic life use for certain streams (see text
box). In these streams, non-native fish, such
as rainbow or brown trout, have been
stocked and are managed by the states as a
sport fishery. Non-native fish do not necessarily
imply poor stream condition, but introduced
species have been known to replace native fish
by direct predation or by out-competing them
for available habitat, food or both. In the
Highlands, approximately 32% of the total
stream length had at least one non-native fish
species present (Figure 23). 17% of streams
had no fish. Again, the lack offish does not
necessarily mean the streams are in poor
                             Definition of Designated Use
  One of the goals of the 1972 Clean Water
  Act was to "restore and maintain the biological
  integrity of the Nation's waters." To
  achieve this goal, the Act calls for the formal
  designation of beneficial uses such as drinking
  water supply, primary contact recreation (e.g.,
  swimming), and aquatic life support (e.g., fish)
  for each stream. Each designated use has a
  unique set of water quality requirements or
  criteria that must be met for the use to be
  attained. The familiar phrase "fishable and
  swimmable" is used to refer to the aquatic life
  support and primary contact recreation
  beneficial use categories. Some states have
  created subcategories of aquatic life use for
  specific types  of fisheries, such as cold or
  warm water, to satisfy the public desires
  to fish for brown trout, rainbow trout, or
smallmouth bass. Often these fish are not
native to the stream or watershed, but rather
have been artificially introduced.

The definition of biotic integrity used to
develop the fish Index of Biotic Integrity
reported in this assessment considers the
stream to be of lower quality or condition if
nonnative fish species are present in the
stream because it is not the "natural" condition
for the stream.  The Clean Water Act does not
define biotic integrity. This is an instance
where the desire to maintain and protect the
natural fisheries and the desire to designate a
stream as an outstanding trout fishery can
come in conflict. Designated stream uses
currently take precedence over the ecological
definition of biotic integrity.
                                               Mid-Atlantic Highlands Streams Assessment T  25

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                           • Native Fish Only
                           O 0 -10% Non-native Fish
                           • >10% Non-native Fish
                           O No Fish
  Figure 23. About 32% of the stream miles
  throughout the Highlands contain normative fish,
  including species stocked and managed for sport
  fisheries.

   health. Some streams, particularly first-order
   streams, do not have fish but do have healthy
   aquatic insect communities.
          SUMMARY RANKING OF

          STRESSORS
          An important part of making future policy
          and management decisions is understanding
          the relative magnitude and extent of current
          stressors. Decision-makers may choose not
          to focus their efforts on the most common
          problems, but knowing which stressors are
          the most widespread should certainly be part
          of the information considered. In Figure 24,
          stressors were ranked according to the
          proportion of stream length impaired or in
          poor quality with regard to that particular
          indicator. The potential stressor that occurs in
          the highest proportion of streams is
          non-native fish (32% of all stream length
          in the Highlands). As discussed earlier in
          this report, many people do not consider
          introduced fish (often sport fish) to be a
          stressor; we list it here to highlight the broad
          extent of non-native fish in the Highlands, and
          leave it up to the reader to decide whether it
  Channel Sedimentation
  Riparian Habitat
  Mine Drainage
  Acidic Deposition
  Fish Tissue Contamination
  Total Phosphorus (Nutrient)
  Total Nitrogen (Nutrient)
  Non-native Fish
                                      ] 25%
                                      24%
                  14%
            11%
          10%
5%

5%
                                            32%
                          0
        10
20
30
                                                % Stream Miles
   Figure 24. Overall ranking of stressors influencing the condition of Mid-Atlantic Highland streams.
26  | Mid-Atlantic Highlands Streams Assessment

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should be considered a stressor in the
same way as other stressors. The next most
common stressors are both elements of
stream physical habitat: channel sedimentation
(25% of stream length) and riparian habitat
disturbance (24% of stream miles). These
stressors are followed by mine drainage
(14%), acid rain (11%), fish tissue
contamination (10%), and stream enrichment
by total phosphorus (5%) and total nitrogen
(5%) (Figure 24).
I
 Habitat destruction is a
     major stressor in
    Highland streams.
                                            Mid-Atlantic Highlands Streams Assessment T  27

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28  |  Mid-Atlantic Highlands Streams Assessment

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     ECOREGIONS, WATERSHEDS, AND STATES:
ANOTHER PERSPECTIVE  ON STREAM CONDITION
 The statistical survey approach, measuring
 multiple indicators of the condition of
 Mid-Atlantic Highland streams, offers us a
 panoramic view of stream quality across the
 Highlands. By knowing first how many streams
 are in poor ecological condition, or are affected
 by particular stressors, we can begin to
 make informed decisions about what level of
 impairment we are willing to accept. In the
 Highlands as a whole, between 27 and 31 % of
 the stream length is in poor ecological condition
 (depending on whether we use aquatic insect
 or fish data to make the
 assessment). The most        i	
 significant stressors (e.g.,
 those that are present in
 the largest proportion of
 stream length) are alterations
 to habitat (in-stream
 and riparian). While this
 assessment is informative,
 most management decisions
 aren't made at the scale or
 perspective of the Highlands
 as a whole.
               ECOREGIONS
               A different, but useful, management
               perspective can be gained by looking at
               stream quality by ecoregions (Figure 25).
               Ecological regions (or ecoregions) are areas
               that have similar soils, vegetation, climate,
               and physical geography. An ecoregion
               perspective highlights the differences, for
               example, between mountain areas with their
               steep slopes, shallow soils, and cool climate,
               and valley areas that are relatively flat, have
               deep soils, and warm temperatures.
 One of the maj or strengths
 of the sample survey design
 used in the Highlands is that
 it can be used and interpreted from various
 management perspectives or scales. We can
 use the same sort of approach (assessing
 first the ecological condition, then identifying
 the major stressors) to look at different
 geographic areas in the region such as
 ecoregions, large watersheds, or states. In this
 section, we present results for these different
 geographic areas, along with some ideas of
 how environmental managers and policy
 makers might choose to use these results.
     An ecoregion
perspective helps us to
    understand why
  streams respond to
    various human
  disturbances as they
     do and which
     management
  solutions might be
      applicable.
               Ecoregional differences
               play a maj or role in
               determining which streams
               have been affected by, or
               are susceptible to, different
               stressors: acid rain, mine
               drainage, and nutrient
               runoff. Management
               practices within an
               ecoregion typically are
               applicable for many
               streams with similar
               problems because the
               stream characteristics in
               the ecoregion are similar.
Some problems or issues are more extensive
in some ecoregions than others.

While looking at the Highlands as a whole
can give us an idea which problems or
stressors most require our attention, a spatial
perspective can help focus management
actions in the geographic areas that most need
help. Focusing restoration efforts where
problems are most extensive can make more
effective use of limited resources.
                                           Mid-Atlantic Highlands Streams Assessment T  29

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r~	4.
    z
T
    Ecoregions
    I  I  Ridge and Blue Ridge
    I  I  North-Central Appalachian
    I  I  Valley
    I  I  Western Appalachian
                                                                                   Atlantic
                                                                                    Ocean
Figure 25. Ecoregions are areas with similar physical geography, soils, climate, and vegetation types. The
Mid-Atlantic Highlands can be represented by four aggregated ecoregions. Ecoregions provide a useful
perspective in viewing stream condition and characteristics (the Highlands area is enclosed by a bold
black outline).
 30  | Mid-Atlantic Highlands Streams Assessment

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WESTERN APPALACHIAN PLATEAU
The Western Appalachian ecoregion runs
from western Pennsylvania into western West
Virginia (Figure 25). The hilly and wooded
terrain of this ecoregion is less rugged and not
as forested as the ecoregions to the east.
Much of this region has been mined for
bituminous coal. Once covered by a maple-
beech-birch forest, this region is now largely
in farms, many of which are dairy operations.
This ecoregion is characterized by low
rounded hills and extensive areas of wetlands.
the worst with respect to fish biotic integrity
(Figures 26 and 27). 37% of stream miles in
the Western Appalachians exhibited poor
scores for the aquatic insect index, and only
2% of stream miles exhibited good scores.
In the case offish, 30% of stream miles had
poor fish biotic integrity (35% of stream
length could not be assessed for fish due to
small stream size); only 3% of stream miles
in the Western Appalachians exhibited good
fish biotic integrity (Figure 26). Environmental
managers in this ecoregion might well decide
     Figure 26. Fish IBI scores ranged from 14% in poor condition in the Ridge and Blue
     Ridge to 43% in poor condition in the North-Central Appalachian ecoregion.
Based on the principles described above, the
ecological condition of streams in the Western
Appalachians would be considered the
poorest of any ecoregion in the Highlands
with respect to aquatic insects, and among
that these results are unacceptable, and
begin looking for ways to improve stream
quality. But where should they begin; which
stressors should they target when they begin
looking for restoration objectives?
                                              Mid-Atlantic Highlands Streams Assessment  T  31

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     Figure 27. Aquatic insect index scores ranged from 14% in poor condition in the
     Ridge and Blue Ridge to 37% in poor condition in the Western Appalachian ecoregion.
   Far and away the most common stressor in
   the Western Appalachians is sedimentation,
   with more than 38% of the stream length in
   the region exhibiting excessive fine sediments
   (Figure 28). Riparian habitat alteration (28%
   of stream length) and mine drainage (24% of
   stream length) are also common in the
   Western Appalachians. High phosphorus
   concentrations are also more common in this
   ecoregion (20% of stream length) than in any
   other. It is very likely that these high-ranking
   stressors are interrelated. While mining is
   most commonly thought of as having a
   chemical impact, it actually has a much larger
   effect on habitat, particularly on rates of
   sediment runoff to streams. Agricultural land
   use, which is common in the Western
   Appalachians, is also correlated with
   sediment problems; these are often
associated with high phosphorus concentrations,
because phosphorus attaches to fine soil
particles and runs off into streams during the
same high discharge storms that carry
sediments into streams. It is very likely that
managers who focused on measures to
control runoff into streams from mines and
agricultural fields could have a major impact
on improving the ecological condition in this
ecoregion.

On the positive side, fish tissue contamination
is found in only 7% of the stream miles and
the effects of acidic deposition and nitrogen
runoff are essentially absent from the Western
Appalachians (Figure 28). Non-native fish
species are less common (19% of stream
length) in the Western Appalachians than in
any other Highland ecoregion.
32  | Mid-Atlantic Highlands Streams Assessment

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NORTH-CENTRAL APPALACHIAN PLATEAU
The North-Central Appalachians in northern
and central Pennsylvania and central West
Virginia (Figure 25) are a vast elevated
plateau of high hills, open valleys, and 1
ow mountains (Figure 28) with sandstone,
siltstone, and shale geology and coal deposits.
Much of the eastern part of the ecoregion is
farmed and in pasture, with hay and grain for
dairy cattle being the principal crops. There
In the North-Central Appalachians, (43% of
stream miles in poor condition) based on fish
IBI scores (Figure 26). The relatively good
scores for aquatic insects in the North-
Central Appalachians (24% of stream miles
in poor condition; 33% in good condition,
Figure 27) almost certainly result from the
fact that sedimentation is much less prevalent
in this ecoregion (only 10% of stream miles
have excessive fine sediments) (Figure 28).
Channel Sedimentation
Riparian Habitat
Mine Drainage
Acidic Deposition
Fish Tissue Contamination
Total Phosphorus (Nutrient)
Total Nitrogen (Nutrient)
Non-native Fish
Channel Sedimentation
Riparian Habitat
Mine Drainage
Acidic Deposition
Fish Tissue Contamination
Total Phosphorus (Nutrient)
Total Nitrogen (Nutrient)
Non-native Fish


0
ZI5
11
]1



I ?8
8 Ridge
and
Blue Ridge
119

• 10
131
1 24



]1
H2


1 24


North-Central
Appalachian
1 36


I 28

I 34
0
H2
116
Zl 3 Valley
lib 40


138
128
124
0
120 Western
Appalachian
119

                         0      10      20      30
                                % Stream Miles
                   10      20     30
                    % Stream Miles
                                                                                      40
Figure 28. Ranking of stressors by ecoregion based on proportion of stream miles scored as
impaired or with poor quality.
also are large areas in oak and
northern hardwood forests. Land use
activities are generally related to forestry
and recreation, but some coal and gas
extraction occurs in the west.
Many aquatic insect species, and other
bottom-dwelling organisms, live in the gaps
and spaces between boulders and gravels
that disappear when sedimentation becomes
a problem in streams.
                                              Mid-Atlantic Highlands Streams Assessment  T  33

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    The most common stressors in the
    North-Central Appalachians are
    riparian habitat alteration (31 % of
    stream length), mine drainage and
    acidic deposition (both are found in
    24% of stream miles in this ecoregion)
    (Figure 28). These are the problems
    that environmental managers might want
    to target for restoration efforts. Introduced
    fish species are also very common in the
    North-Central Appalachian Plateau,
    with 36% of stream miles having one or
    more non-native species. Nutrient
    runoff (<2% of stream length affected)
    is relatively unimportant in this largely
    non-agricultural ecoregion.

    VALLEY
    The Valley ecoregion extends from
    eastern Pennsylvania southwesterly
    through southwestern Virginia (Figure 25).
    The valleys generally are of two types,
    those underlain by limestone and those
    by shale. The nutrient rich limestone
    valleys contain productive agricultural
    land (Figure 29). By contrast, the shale
    valleys are generally less productive,
    more irregular, and have greater
    densities of streams. Most of the
    streams in the limestone valleys are
    colder and flow all year, whereas those
    in the shale valleys tend to lack flow in dry
    periods. Large poultry operations can be
    found in many parts of the valleys.
    The proportion of stream length in poor
    ecological condition in the Valley based on
    aquatic insect scores (36%) was comparable
    to the poor stream condition measured in the
    WesternAppalachians (Figure 27). Fish
Figure 29. The Valley ecoregion extends from eastern
Pennsylvania southwesterly through southwestern
Virginia and contains productive agricultural land.
      assemblage scores indicated about 31% of
      the stream length was in poor ecological
      condition in the Valley (Figure 26).

      Over one-third of the stream miles (34%) had
      poor scores for riparian habitat alteration and
      28% of the stream miles were scored poor
      for excess sedimentation (Figure 28). Physical
      habitat alteration, both riparian habitat and
      excess sedimentation, almost certainly
      contributed to the poor biological condition of
34  | Mid-Atlantic Highlands Streams Assessment

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the streams. The aquatic insects are particularly
sensitive to excess sedimentation. Non-native
fish species were found in about 40% of
the stream miles in the Valley. Fish tissue
contamination (16%) and total nitrogen
(15%) each represent about half the number
of stream miles associated with poor scores
for physical habitat alteration. Agriculture
and poultry operations might be contributing
to the elevated nitrogen concentrations. Total
phosphorus concentrations and acid rain are
associated with less than 3% of the stream
miles and mine drainage is not a problem in
the Valleys. Managers might target physical
habitat restoration in the Valley ecoregion to
reduce this major environmental problem.
Targeting physical habitat restoration should
also help reduce nitrogen runoff.

RIDGE AND BLUE RIDGE
The Ridge and Blue Ridge ecoregion is a
series of linear mountainous ridges with
elevations from approximately 1,000 feet to
5,700 feet (Figure 25). This mostly forested
ecoregion contains cool, clear streams with
steep slopes which occur over mostly
sandstone and shale bottoms. The ecoregion
has no major urban
areas and has a low
population density.
However, due in large
part to the close proximity
of metropolitan areas to
the east (Philadelphia,
Baltimore, Washington,
D.C., Richmond) (see
inside front cover), recreational development
in the region has increased considerably in
recent years.
                        Only the Ridge and
                       Blue  Ridge ecoregion
                        has less than 25% of
                        the stream miles in
                          poor condition.
 Habitat destruction
is a major stressor in
   every ecoregion.
The Ridge and Blue-Ridge had the smallest
number of stream miles with fish assemblage
(14%) (Figure 26) and aquatic insect scores
(14%) (Figure 27) indicating poor ecological
condition. This region also had the greatest
number of stream miles in good ecological
condition based on fish IBI (28%) and
aquatic insect (46%) scores. The Ridge and
Blue-Ridge region is relatively undeveloped,
with the predominant land use being forests.

Even in this region, however, about 19%
of the stream miles have non-native species
and 28% of the stream miles have excess
sedimentation (Figure 28). The other
stressors - poor riparian habitat, acidic
deposition, fish tissue contamination and total
              phosphorus and total
              nitrogen concentrations - are
              each associated with less
              than 10% of the stream
              miles. Mine drainage is not a
              problem in the Ridge and
              Blue-Ridge region.
                               COMMON THEMES
                               Physical habitat alteration -
                 both riparian habitat and excess channel
                 sedimentation - were prevalent and common
                 in all the ecoregions. Targeting physical
                 habitat restoration would reduce a major
                                             Mid-Atlantic Highlands Streams Assessment T 35

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   potential stressor throughout the Highlands
   and in all of the ecoregions.

   WATERSHEDS
   Watersheds are considered the primary
   management unit for many aquatic problems
   (Figure 30). Sediment transport and
   sedimentation, increased nutrient and
   contaminant loading, and similar problems are
   associated with water running off the land
   and washing sediment, nutrients and
   contaminants into the stream. Considering
   stream condition by watersheds can indicate
   which watersheds might be high priority
candidates for management and restoration.
Stressors can also be ranked by watersheds
just as they were by ecoregions.

CHESAPEAKE WATERSHED
A major portion of the Chesapeake Bay
watershed is contained in the Mid-Atlantic
Highlands. Management practices in the
Highlands portions of the Chesapeake
watershed can help control problems in
Chesapeake Bay. The Chesapeake Bay
Program has recently implemented tributary
strategies to improve conditions in the Bay.
Both fish (Figure 31) and aquatic insect
               Figure 30.  Three watersheds or combined drainage basins can be
               assessed in the Mid-Atlantic Highlands. A watershed perspective is useful
               in viewing stream condition.
36  | Mid-Atlantic Highlands Streams Assessment

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assemblages (Figure 32) indicated about 23
and 20%, respectively, of the stream miles
in that portion of the Chesapeake Bay
watershed in the Highlands were in poor
ecological condition. The fish IBI scores for
the Chesapeake watershed indicated that
about 25% of the stream miles were in good
condition, while the aquatic insect EPT
scores indicated that about 32% of the
stream miles were in good condition.

Channel sedimentation (25%) and riparian
habitat alteration (12%) were the stressors
that were associated with the highest
proportion of stream miles in poor quality in
the Chesapeake watershed (Figure 33).
Other stressors of concern included acidic
deposition (11%), total nitrogen (7%), and
fish tissue contaminants (5%). Mine drainage
and total phosphorus were not major
stressors in the Chesapeake watershed.
Non-native fish were found in 31% of the
stream miles. The Chesapeake Bay Program
has implemented a goal of 2,010 miles of
riparian restoration by 2010. This goals
applies to the entire Chesapeake watershed
and only a portion of the restoration will occur
in the Highlands region. Initial restoration
effects are targeted toward planting trees,
which should not only improve riparian
habitat, but also help reduce excess channel
sedimentation. These are two of the biggest
problems in the Highland portion of the
Chesapeake watershed.

AlXEGHENY-MONONGAHELA WATERSHED
The Allegheny and Monongahela Rivers join
at Pittsburgh, PA to form the Ohio River
       Figure 31. Fish IBI scores for streams in the Highland portions of three watersheds.
       Strong differences in the condition offish assemblages exist among the three
       watersheds.
                                             Mid-Atlantic Highlands Streams Assessment  T  37

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   (Figure 30). This watershed is found in
   the middle area of the Highland region.
   Managing aquatic problems in the upper
   watersheds can help reduce problems in the
   Ohio River, the Mississippi River, and
   ultimately the Gulf of Mexico.

   About 31% of the stream miles were scored
   in poor ecological condition using the fish
   IBI index (Figure 31) while about 22% of
   the stream miles were scored in poor
   condition using the aquatic insect index
assemblages are typically more sensitive to
the riparian habitat, acidic deposition, and
mine drainage stressors.

Riparian habitat alteration (28%), acidic
deposition (26%), mine drainage (20%), and
fish tissue contamination (19%) were the
major stressors associated with stream miles
in the Allegheny-Monongahela watershed
(Figure 33). All of these stressors were
associated with at least 20% of the stream
miles in this watershed. Non-native fish
                 Figure 32. EPT scores for streams in three Highland watersheds.
   (Figure 32). The fish index indicated that
   only about 11 % of the stream miles were in
   good condition while the aquatic insect index
   indicated that 27% of the stream miles were
   in good condition. Some of these differences
   are likely due to the stressors that are
   associated with the streams in the
   Allegheny-Monongahela watershed. Fish
species were found in 46% of the
stream miles in this watershed. Channel
sedimentation (6%), total phosphorus (2%),
and total nitrogen (2%) concentrations were
not major stressors in this watershed. The
fish index scores indicated a greater number
of stream miles were in poor condition with
fewer stream miles in good condition than
38  | Mid-Atlantic Highlands Streams Assessment

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 Channel Sedimentation
 Riparian Habitat
 Mine Drainage
 Acidic Deposition
 Fish Tissue Contamination
 Total Phosphorus (Nutrient)
 Total Nitrogen (Nutrient)
 Non-native Fish
 Channel Sedimentation
 Riparian Habitat
 Mine Drainage
 Acidic Deposition
 Fish Tissue Contamination
 Total Phosphorus (Nutrient)
 Total Nitrogen (Nutrient)
 Non-native Fish




I2b

1 12
]2_


Chesapeake
]7
131

ISO
ISO



0


121

I I O „
upper Uhio
I2U

=|6
I 28
I2U
I 26
I 19 . „ ,
n 2 Allegheny-
-| 2 Monongahela
1 46

0 10 20 30 40 5
% Stream Miles
                              10     20     30    40
                              % Stream Miles
Figure 33. Ranking of stressors among streams in three Highland watersheds. While the general
ranking of stressors is similar, there were differences among watersheds.
the aquatic insect scores. Using multiple
indicators or indices provides a better
picture of stream condition than any one
single indicator or index.

KANAWHA-UPPER OHIO WATERSHED
The Kanawha-Upper Ohio watershed is
located in the southern and western portion
of the Highland region (Figure 30). About
21% of the stream miles in this watershed
were scored in poor condition using the fish
IBI (Figure 31) and about 36% of the
stream miles were scored in poor condition
using the aquatic insect index (Figure 32).
The number of stream miles in good
condition were scored similarly by the fish
(12%) and aquatic insect indices (14%).
Channel sedimentation (30%) and riparian
habitat alteration (30%) are the two stressors
that were found in the greatest number of
stream miles throughout the Kanawha-Upper
Ohio watershed (Figure 33). These two
stressors were followed by mine drainage
(21 %) and non-native fish (20%). Total
phosphorus concentrations above the EPA
guideline were associated with about 13% of
the stream miles, the highest proportion found
for any of the three watersheds. Fish tissue
contamination was found in about 9% of the
stream miles where there was sufficient fish
tissue to measure contaminants. Acidic
deposition was an issue in about 7% of the
stream length and elevated total nitrogen
concentrations were found in less than 1% of
the stream miles in this watershed. The
                                              Mid-Atlantic Highlands Streams Assessment  T  39

-------
    aquatic insect index is particularly sensitive to
    channel sedimentation, which likely explains
    why there were a greater proportion of
    stream miles scored in
    poorcondition with this
    index. Again, this reinforces
    the importance of using
    multiple indicators or
    indices to determine stream
    condition.
J_
    COMMON THEMES
    Riparian habitat alteration
    was a high ranking
    potential stressor in each
    of the three watersheds.
    Channel sedimentation was a high ranking
    potential stressor in the Chesapeake and
    Kanawha-Upper Ohio watersheds, while mine
    drainage was a high ranking potential stressor
    in the Allegheny-Monongahela and Kanawha-
    Upper Ohio watersheds. Management
    practices should be targeted to these high
    ranking stressors in the various watersheds.
    Targeting channel sedimentation and riparian
    habitat should also reduce total nitrogen and
    total phosphorus concentrations. Targeting
    mine drainage also will reduce excess channel
    sedimentation. Stream restoration practices for
    these problems include re-establishing bank
    vegetation, putting riffles back in the stream
    channel, and adding boulders and tree trunks to
    stabilize the channel. Re-establishing vegetative
    buffer zones along the stream bank helps reduce
    sediment and nutrient loads to the streams.

    STATES
    Management at the state level is critical for
    effective environmental protection, management,
 Stream miles in poor
   condition ranged
   from 23% in the
      Chesapeake
 watershed to 41% in
 the Kanawha-Upper
    Ohio watershed.
and restoration. In many instances, the
management units for the states also are
ecoregions and watersheds. Because West
                 Virginiais fully within the
                 boundaries of the High-
                 lands and most of Pennsyl-
                 vania is located in the
                 Highland region, the
                 statistical survey design
                 results are also applicable
                 for these two states.
                 Management insights can
                 be gained by looking at the
                 condition of streams, and
                 the ranking of stressors in
                 these two states.

PENNSYLVANIA
The number of miles of stream scored in poor
ecological condition was 27 % using both the fish
(Figure 34)andaquaticinsectindices (Figure 35).
The number of stream miles scored in good
condition by the aquatic insect index was 25%
compared with 14% of the stream miles scored
in good condition by the fish IBI.

Riparian habitat alteration and channel
sedimentation were associated with 21 % and
19% of the stream miles, respectively, in
Pennsylvania (Figure 36). Mine drainage, acidic
deposition, and fish tissue contamination were
also associated
with about 15%
of the stream
miles. Nutrient
concentrations of
                both total
                phosphorus and
                total nitrogen
                were stressors
      Habitat
destruction was a
major stressor in
 all  watersheds.
40  | Mid-Atlantic Highlands Streams Assessment

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Figure 34. Comparison of Fish Index of Biotic Integrity scores between Pennsylvania
and West Virginia showing proportion of stream miles in good, fair, and poor conditions
based on a best attainable reference.
   Figure 35. Comparison of aquatic insect scores for Pennsylvania and West Virginia
   streams.
                                       Mid-Atlantic Highlands Streams Assessment T 41

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              Channel Sedimentation
              Riparian Habitat
              Mine Drainage
              Acidic Deposition
              Fish Tissue Contamination
              Total Phosphorus (Nutrient)
              Total Nitrogen (Nutrient)
              Non-native Fish
              Channel Sedimentation
              Riparian Habitat
              Mine Drainage
              Acidic Deposition
              Fish Tissue Contamination
              Total Phosphorus (Nutrient)
              Total Nitrogen (Nutrient)
              Non-native Fish
        21
   J16
    15
           Pennsylvania
                         346
           H26
 IMS
 H14

 ZM5
 West
Virginia
                                               i
                                              10
                                                     20
              l
              30
    l
   40
                                                                            50
                                                  % Stream Miles
        Figure 36. Comparison of stressors in streams between Pennsylvania and West Virginia.
        In general, the stressors in Highland streams in these two states were similar, except for
        total nitrogen enrichment and fish tissue contamination.
   in about 10% of the stream miles. Pennsylvania
   has many stocked trout streams so non-native
   fish were found in 44% of the stream miles.

   WEST VIRGINIA
   In West Virginia, 44% of the stream miles
   were scored in poor ecological condition
   using the fish IBI (Figure 34) compared with
   25% of the stream miles scored in poor
   condition using the aquatic insect index
   (Figure 35). Similar differences were
   observed for the number of stream miles
   scored in good condition. The fish IBI
   scores indicated 13% of the stream miles
   were in good ecological condition compared
   with 20% scored in good condition using the
   aquatic insect index.
Physical habitat alteration - (26%) riparian
habitat and excess channel sedimentation
(18%) - were the two highest ranked
stressors in West Virginia streams
(Figure 36). Mine drainage and acidic
deposition were each associated with about
14% of the stream miles. Total phosphorus
concentrations rarely exceeded the EPA
guidelines in West Virginia streams and fish
tissue contamination and total nitrogen
concentrations in the poor category were
associated with 1% or less of the stream
miles. Non-native fish species were found in
26% of the stream miles in West Virginia.
42  | Mid-Atlantic Highlands Streams Assessment

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COMMON THEMES
Physical habitat alteration (both excess
channel sedimentation and riparian habitat)
are major stressors in both states. Mine
drainage and acidic deposition are also
stressors that are common in both states,
and are associated with similar numbers of
stream miles. Management practices in both
states might be targeted to these stressors.
Non-native fish species also were found in at
least 25% of the stream miles in both states.
      Habitat destruction is
      also a major stressor in
      both Pennsylvania and
           West Virginia.
                                            Mid-Atlantic Highlands Streams Assessment  T 43

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44  |  Mid-Atlantic Highlands Streams Assessment

-------
               DEVELOPING A SCORECARD:
          SUMMARIZING STREAM CONDITION
The statistical survey approach, measuring
multiple indicators of the ecological condition
of Mid-Atlantic Highland streams, offers a
panoramic view of stream quality across the
Highlands. By knowing, first, how many
streams are in poor ecological condition, and
the ranking of the stressors, we can begin to
make informed decisions about what levels
of impairment we will tolerate and how to
target management and restoration efforts to
address those streams whose level of
impairment is not acceptable. The following
example illustrates how this information can
be integrated across the Highlands, how
tolerable and intolerable levels of impairment
might be defined, how a score card can be
developed, and how it might be used to
target geographic areas for management.

For this exercise, let's suppose that if less
than 10% of the streams are scored in poor
ecological condition we can tolerate the
situation. We don't desire impaired streams,
but we can tolerate it if less than 10% of the
streams are in poor ecological condition.
However, if the proportion of stream miles
scored in poor condition is between 10 and
25%, a yellow flag is raised. This yellow flag
indicates that additional monitoring of change
over time is needed to determine if this
percentage is increasing or decreasing. If it is
increasing, decision makers, managers and
the public need to evaluate this situation and
decide if this represents a high priority for
management. If it is decreasing, current
management practices should be continued.

If more than one-quarter - 25% - of the
stream miles are in poor ecological condition
based on any biological index, regional





Overall,
Highland
stream
condition is
poor.
stream quality would be considered
unacceptable and management or policy
actions are needed now. (NOTE: only
biological indicators or indices are used to
determine stream ecological condition.)

Similar thresholds can be used to rank the
stressors (e.g., less than 10% of the stream
miles scored poor are tolerable, between 10
and 25% of the
stream miles raises a
warning flag, and over
25% of the stream
miles might require a
management action if
the potential stressor
is associated with
poor ecological
condition). The only exception to these
thresholds is for fish tissue contaminants, which
have both human health and ecological health
implications. For fish tissue contaminants, let's
assume that less than 2% of the stream miles
with fish tissue contaminants is tolerable,
between 2 and 10% is a warning zone, and
that greater than 10% of the stream miles with
fish tissue contaminants is intolerable. We can
use this information to rank stressors similar to
the ranking of stream ecological condition.
By using these thresholds, we can prepare a
color-coded table or scorecard (Table 3) -
green for
tolerable, yellow
for warning, and
red for unac-
ceptable-that
pulls all this
information
J_
    Scorecards
 integrate stream
  condition and
     stressors.
                                           Mid-Atlantic Highlands Streams Assessment T  45

-------
    together. In addition, we can summarize the
    information in the same table for different
    geographic areas and management
    perspectives - i.e., the entire
    Highland region, ecoregions,
    watersheds or states.
                             J_
                                  Physical habitat
                                  destruction is the
                                  greatest potential
                                       stressor.
Based on the score card in
Table 3, the overall ecological
condition of the region is
poor, for both biological
indices. In fact, the only
management areain which
ecological condition is not poor is the Ridge &
Blue Ridge ecoregion, where ecological
conditionisfair.

Physical habitat alteration, overall, represents
that greatest potential stressor in almost all
the geographic areas as well as across the
Highland region as a whole. The other
stressors, in general, can be targeted in specific
geographic management areas, but are not
wide-spread across the entire Highland. Some
chronic problems such as mine drainage and
acid rain still persist. However, nutrient
problems are not as common in Highland
streams as in other regions of the country.
            Non-native fish species might
            be the greatest potential
            stressor to the Highlands region
            overall, or it might represent a
            success story for trout,
            smallmouth bass, or similar
            fisheries management
            programs. There are societal
            arguments on both sides of this
            issue - loss of biological
integrity versus improved recreational
opportunities. This will continue to be, and
should be, a topic of public discussion.

Score cards such as Table 3 can be used to
target geographic areas with high priority
environmental problems for implementing
management practices. A scorecard helps
pull information together and display it so
that informed decisions can be made on
where to target geographic areas and how to
prioritize management practices.
46  |  Mid-Atlantic Highlands Streams Assessment

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                                                  Table 3. Scorecard for Mid-Atlantic Highland Streams.
Condition &
Stressor Ranking
Ecological Conditions
- Fish
- Aquatic Insects
Highland
Region
•

Ecoregions
Western
Appalachian
NC-
Appalachian
Valley
Ridge &
Blue Ridge
Watersheds
Chesapeake
Allegheny-
Monongahela
Kanawha
Upper Ohio







14
14
23 1
20
22


States
PA
WV
I
1 1
Potential Stressors
Habitat
- Channel Sedimentation
- Riparian Streambank
Water Quality
- Mine Drainage
- Acid Rain
- Total Phosphorus
- Total Nitrogen
Fish Contaminants
- Carcinogens
- Mercury
Biological
- Non-Native Species


24

14
11


•H
4

32




24

20


7
6

19

10


24
24




8

36







15




40









5


19


12


11



5
4

31




20






46




21

13


9


20

19
21

16
14


DB
9

44

18
	
	
13
14


	

26
 o-
 M
 GO
t
Note: Green = <10% of stream miles ranked as poor. Yellow = 10 to 25% of stream miles ranked as poor. Red = >25% of stream miles ranked as poor. Normative
species are managed in some streams so they are ranked as neutral.
*Fish Contaminants
Green = <5% of stream miles with any contaminant in fish tissue.
Yellow = 5 to 10% of stream miles with an contaminant in fish tissue.
Red = >10% of stream miles with any contaminant in fish tissue.

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48  |  Mid-Atlantic Highlands Streams Assessment

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               MANAGEMENT IMPLICATIONS
Some of the management implications
from assessing the ecological condition of
Highland streams are:

   *°  You can't play the game without
       a scorecard. A score card can help
       pull all the information together and
       indicate environmental problems and
       target areas for management.

   *°  You can't develop a scorecard
       using existing monitoring
       networks. Statistical stream survey
       designs are a cost-effective and
       efficient way to get information on
       the condition of streams in the
       Mid-Atlantic region. Survey designs
       complement, not replace, existing
       monitoring networks.

   ^  A single indicator only tells part
       of the story. Multiple indicators and
       indices are needed both to determine
       stream ecological condition and to
       identify stressors affecting stream
       condition. Biological indicators or
       indices should be used to assess
       ecological condition.
Chemical indicators don't tell the
whole story. Biological indicators
and indices integrate across physical
and chemical indicators.

Just one management
perspective is not enough.
Different management perspectives -
region-wide, ecoregion, watershed,
state levels - are important in
determining where the high-priority
environmental problems are and
where to target management efforts.

You can't evaluate the success of
management actions without
repeated monitoring.
Monitoring the change in stream
condition after management actions
have been taken is the only
approach for evaluating the success
of these actions. Unfortunately,
monitoring networks are generally
cut-back or discontinued when
budgets get tight.
                                           Mid-Atlantic Highlands Streams Assessment  T 49

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50  |  Mid-Atlantic Highlands Streams Assessment

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           WHERE Do WE Go FROM HERE?
There has been progress since the passage
of the Clean Water Act in 1972. Point
source discharges are now permitted, best
management practices are being implemented
to control nonpoint source runoff, and
restoration activities are underway throughout
the Highlands. Yet, over one-quarter of the
stream miles in the Highlands are in poor
condition. We still have a ways to go.

Habitat degradation is occurring across the
Highlands and is affecting the condition
and quality of our streams. Conversion of
forest land into other land uses (e.g.,
agriculture, housing developments,
commercial developments) and fragmentation
of the forests (road building, parceling areas
into lots) represents one of the greatest
sources of habitat degradation. "Smart
growth " is  no longer just a catchy phrase;
it is a necessity if we are to protect and
improve stream condition throughout the
region. Best management practices must
accompany these conversions for all types
of land use.

Based on the results of this assessment,
targeted management practices can be
identified for different ecoregions,
watersheds, and states. This will include
protecting streams in some areas such as
the Ridge and Blue Ridge ecoregion,
managing stream corridors in areas such as the
Chesapeake watershed, and restoring streams
in West Virginia that have been degraded by
mine drainage.

To be able to assess future progress, we
must continue to monitor stream condition.
Monitoring is typically one of the first
activities to be cut when budgets get tight.
Yet, monitoring is the only way we can
assess our progress and determine if our
management practices are effective. The
statistical survey approach used in this study
is an innovative, cost-effective way of
complementing other monitoring networks
and provide the kind of information we need
to determine if we are making a difference.

Together, we can make a difference in
protecting, managing, and restoring Highland
streams, but it will take a team effort. Just as
we worked together with partners from the
states, other agencies, universities and the
private sector to conduct this assessment, so
do we need to continue this partnership to
improve stream conditions in the future.
Coming together is a beginning; staying
together is progress; working together is
success. We can, if we chose, be successful
in improving stream conditions throughout
the Mid-Atlantic region - we simply need the
resolve to get it done.
                            Together we can make
                             a difference, but we
                             must work together.
                                            Mid-Atlantic Highlands Streams Assessment T  51

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52
Mid-Atlantic Highlands Assessment

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APPENDIX A: ADDITIONAL  READINGS

Technical Journal Articles in Support of the Highlands stream report:

Boward, D., Kazyak, P., Stranko,  S., Kurd, M., and Prochaska, A (1999). From the Mountains
       to the Sea: The State of Maryland's Freshwater Streams. EPA/903/R-99/023, US
       Environmental Protection Agency, Philadelphia, PA.
Bradley, M.P. and Landy, R.B. (2000). The Mid-Atlantic Integrated Assessment (MAIA).
       Environmental Monitoring Assessment 63:1-13.
Bradley, M.P., Brown, B.S., Hale, S.S., Kutz, F.W., Landy, R.B., Shedlock, R.J., Mangold, R.P.,
       Morris, A.R., Galloway, W.B., Rosen, J.S., Pepino, R., and Wiersma, G.B. Summary of
       the MAIA Working Conference. Environmental Monitoring Assessment 63:15-29.
Bryce, S.A., Larsen, D. P., Hughes, R. M., and Kaufmann, P.R. (1999). "Assessing the relative
       risks to aquatic ecosystems in the Mid-Appalachian region of the United States."
       Journal of the American Water Resources Association, 35, 23-36.
Bryce, S.A., Larsen, D. P., Hughes, R.M., and Kaufmann, P.R. (1999). "Assessing relative risks
       to aquatic ecosystems: a Mid-Appalachian case study." Journal of the American
       Water Resources Association, 35, 23-36.
Herlihy, A.T., Larsen, D.P., Paulsen, S.G., Urquhart, N.S., and Rosenbaum, B.J. (2000).
       "Designing a spatially balanced, randomised site selection process for regional stream
       surveys: The EMAP Mid-Atlantic Pilot Study." Environmental Monitoring and
       Assessment. 63:95-113.
Herlihy, A.T., Stoddard, J.L., and  Johnson, C.B. (1998). "The relationship between stream
       chemistry and watershed land use data in the mid-Atlantic region, U.S." Water Air and
       Soil Pollution, 105, 377-386.
Hughes, R.M., Kaufmann, P.R., Herlihy, A.T., Kincaid, T.M., Reynolds, L., and Larsen, D.P.
       (1998). "A process for developing and evaluating indices offish assemblage integrity."
       Canadian Journal of Fisheries and Aquatic Science,  55, 1618-1631.
Hughes, R.M. (1995). Defining acceptable biological status by comparing with reference
       conditions. In W. Davis and T Simon,  eds., Biological assessment and criteria:  Tools
       for water resource planning and decision making. Chelsea, MI:  Lewis.
Hughes, R.M., andR.F. Noss. (1992). Biological diversity and biological integrity: current
       concerns for lakes and streams. Fisheries 17(3): 11-19.
Jones, K.B., Riitters, K. H., Wickham, J.D., Tankersley, R.D., O'Neill, R.V., Chaloud, D.J.,
       Smith, E.R., and Neale, A.C. (1997). "An Ecological Assessment of the Unites States
       Mid-Atlantic Region: A Landscape Atlas." EPA/600/R-97/130, U.S. Environmental
       Protection Agency, Washington, DC.
Kaufmann, P.R., Levine,  P., Robison, E.G., Seeliger, C., and Peck, D. (1999). "Quantifying
       Physical Habitat in Wadeable Streams." EPA/620/R-99/003, U.S. EPA, Washington,
       DC.
                                                 Mid-Atlantic Highlands Assessment    T  53

-------
  Landers, D.H., R.M. Hughes, S.G. Paulsen, D.P. Larsen, and J.M. Omernik. (1998). How can
         regionalization and survey sampling make limnological research more relevant?
          Verhandlungen Internationale  Vereinigung fur Theoretische und Angewandte
         Limnologie 26: 2428-2436.
  Larsen, D.P., and Herlihy, A.T. (1998). "The dilemma of sampling streams for
         macroinvertebrate richness." Journal of the North American Benthological Society,
          17,359-366.
  Lazorchak, J.M., Klemm, D.J., and Peck, D.V. (1998). "Environmental Monitoring and Assess-
         ment Program - Surface Waters: Field Operations and Methods for Measuring the
         Ecological Conditions of Wadeable Streams." EPA/620/R-94/004, U.S. Environmental
         Protection Agency, Washington, DC.
  McCormick, F.H., Hughes, R.M., Kaufmann, P.R., Herlihy, A.T., and Peck, D.V. (In Press).
         "Development of an index of biotic integrity for the Mid-Atlantic Highlands region." .
  Olsen, A.R., Sedransk, J., Edwards, D., Gotway, C.A., Liggett, W., Rathbun, S., Reckhow, K.H.,
         and Young, L.J. (1999). "Statistical issues for monitoring ecological and natural re-
         sources in the United States." Environmental Monitoring and Assessment, 54, 1-45.
  Paulsen, S.G, Hughes, R.M., and Larsen, D.P. (1998). "Critical elements in describing and
         understanding our nation's aquatic resources." Journal of the American Water Resources
         Association, 34,  995-1005.
  Plafkin, J.L., Barbour, M.T., Porter, K.D., Gross, S.K., and Hughes, R.M. (1989). "Rapid
         Bioassessment Protocls for Use in Streams and Rivers: Benthic Macroinvertebrates and
         Fish." EPA/444/4-89-001, U.S. Environmental Protection Agency, Washington DC.
  Roth, N.E., M.T. Southerland, G Mercurio, J.C. Chaillou, D.G Heimbuch, J.C. Seibel. (1999).
         State of Streams: 1995-1997 Maryland Biological Stream Survey Results. Annapolis,
         MD: Maryland Departmentof Natural Resources.
  Roth, N., and eight coauthors. (1998). Maryland biological stream survey: development of a fish
         index of biotic integrity. Environmental Monitoring and Assessment, 51: 89-106.
  Stevens, D.L., Jr.,. (1994). "Implementation of a national monitoring program." Journal of
         Environmental Management, 42, 1-29.
  Stevens, D.L., Jr.,. (1997). "Variable  density grid-based sampling designs for continuous spatial
         populations." Environmetrics, 8, 167-195.
  Thornton, K.W., and Paulsen, S.G. (1998). "Can anything significant come out of monitoring?"
         Human and Ecological Risk Assessment,  4, 797-805.
  Winter, B.D. and R.M. Hughes. (1997). AFS position statement on biodiversity. Fisheries,
         22(l):22-29.
54  |    Mid-Atlantic Highlands Assessment

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Other MAIA Reports:

Jones, K.B., K.H. Riitters, J.D. Wickham, R.D. Tankersley, R.V. O'Neill, D.J. Chaloud, E.R.
     Smith, A.C. Neale. 1997. An Ecological Assessment of the United States Mid-Atlantic
     Region: A Landscape Atlas. US Environmental Protection Agency, Office of Research and
     Development, Washington, DC 20460. EPA/600/R-97/130.

US Environmental Protection Agency. (1998). Condition of the Mid-Atlantic Estuaries. US
     Environmental Protection Agency, Office of Research and Development, Washington, DC
     20460. EPA/600/R-98/147.

MAIA Web-based Documents:

US Environmental Protection Agency. (2000). Mid-Atlantic Highlands Streams Assessment:
     Technical Support Document US Environmental Protection Agency, Office of Research and
     Development and Region 3, Mid-Atlantic Integrated Assessment Program, Ft. Meade, MD
     20755.

Other EPA Reports:

Guidelines for Preparation of the  1996 State Water Quality Assessments  [305(b) Reports] US
     Environmental   Protection  Agency   EPA 841 B-95-001.   Washington,  DC.

Additional Mid-Atlantic Highlands Reading:
Ator, S.W., and Ferrari, M.J. (1997). Nitrate and selected pesticides in ground water of the
       Mid-Atlantic region, U.S. Geological Survey.
Bryce,  S.A., Omernik, J.M., and Larsen, D.P. (1999). Ecoregions: A geographic framework to
       guide risk characterization and ecosystem management. Journal of the National
       Association of Environmental Professionals Practice 1:142-155.
Hill, B.H., Herlihy, A.T., Kaufmann, P.R., and Sinsabaugh, R.L. (1998). "Sediment microbial
       respiration in a synoptic survey of Mid-Atlantic streams." Freshwater Biology, 39,
       493-501.
Hill, B.H., McCormick, F.H.,  Stevenson, R.J., Herlihy, A.T., and Kaufmann, P.R. (In review).
       "The use of periphyton assemblage data in an index of biotic integrity."
Pan, Y., Stevenson, R.J., Hill, B.H., Herlihy, A.T., and Collins, G.B. (1996). "Using diatoms as
       indicators of ecological conditions in lotic systems: a regional assessment." Journal of
       the North American Benthological  Society, 15.
Pan, Y., Stevenson, R.J., Hill, B.H., Kaufmann, P.R., and Herlihy, A.T. (1999). "Spatial patterns
       and ecological determinants of benthic algal assemblages in Mid-Atlantic Highlands
       streams." Journal of Phycology, 35, 460-468.
Websites:
MAIA - www.epa.gov/maia
EMAP - www.epa.gov/emap
                                                   Mid-Atlantic Highlands Assessment    T  55

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 ffi



f

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 ffi
f
 CT>

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Appendix B:   Table Bl. Percent of stream miles in good condition or affected by potential stressors for the Mid-Atlantic High-
              lands, four Highland ecoregions, three watersheds, and two states.
MK>-
R
Constituents
Fish IBI1
EPT Index1
Normative Fish2
Fish Tissue Contamination3
- Carcinogens
- Mercury
Mine Drainage4
Acidic Deposition4
Total Nitrogen6
Total Phosphorus5
Riparian Habitat
Instream Habitat
Watershed Condition1
§
£
1
o'
ffi
t
o-
\
Assessment

ATLANTIC
ECOREGION
EGION
North-Central Ridge &
Western
Appalachians Blue Ridge Valley Appalachians
17
25
48

46
52
86
89
85
90
48
35
45

15
33
52

66
70
76
76
93
97
40
50
52

28
46
61

36
41
100
92
98
95
92
47
77

23
16
52

37
53
100
98
70
89
19
27
18

4
3
37

41
41
76
100
68
74
35
21
2

WATERSHED
Allegheny- Kanawha-TJpper
Chesapeake Monongahela
25
32
59

47
48
97
89
85
95
52
31
45

11
27
42

59
64
81
74
91
89
58
48
47

Ohio
12
14
57

48
57
79
94
83
83
39
29
48

1 % stream miles in good condition
2 % stream miles without normative fish
3 % stream miles without at least one constituent above human health carcinogen criteria or above mammalian mercury criteria
4 % stream miles not affected
5 % stream miles with TP<50 ug/L EPA guideline
6 % stream miles with TN < 1,300 ug/L based on EPA TP guideline



























                                                                                                        	STATE	

                                                                                                                   West
                                                                                                        Pennsylvania Virginia
                                                                                                            14        13
                                                                                                            25        20
                                                                                                            46        58
                                                                                                            53
                                                                                                            59
                                                                                                            84
                                                                                                            86
                                                                                                            73
                                                                                                            84
                                                                                                            46
                                                                                                            33
                                                                                                            35
56
57
87
93
41
43
64
                                                                                                                             "'••',
                                                                                                                             111

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        Appendix B:   Table B2. Percent of stream miles in fair condition or affected by potential stressors for the Mid- Atlantic
                        Highlands, four Highland ecoregions, three watersheds, and two states.
MID-ATLANTIC
   REGION
                                                       ECOREGION
WATERSHED
                                                                                                                               STATE
Constituents
Fish IBI1
EPT Index1
Normative Fish
Fish Tissue Contamination
- Carcinogens
- Mercury
Mine Drainage
Acidic Deposition
Total Nitrogen2
Total Phosphorus3
Riparian Habitat1
Instream Habitat1
Watershed Condition1
        North-Central  Ridge &          Western
        Appalachians  Blue Ridge Valley Appalachians
36           32         44      37        32
48           43         41      48        61
Chesapeake
    39
    48
  Allegheny-   Kanawha-
 Monongahela Upper Ohio  Pennsylvania
51
52
                                                                                      26
                                                                                      50
                              46
                              48
                                                                                                                                       West
                                                                                                                                     Virginia
                                                                                                                                        20
                                                                                                                                        55
10
5
28
40
30
5
2
28
40
21
<1
4
3
26
13
15
8
48
45
48
24
6
37
51
4
8
4
36
44
35
7
9
14
46
17
17
4
31
41
30
19
8
34
48
27
2
2
33
39
28
         1    % stream miles in fair condition
         2    % stream miles with TN > 1,300 Ug/L but < 3,000 Ug/L based on TP guideline
         3    % stream miles with 1 00 > TP > 50 Ug/L EPA guideline
ft

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Appendix B: Table B3. Percent of stream miles in poor condition or affected by potential stressors for the Mid-Atlantic
             Highlands, four Highland ecoregions, three watersheds, and two states.
MID-ATLANTIC
North-Central
Constituents
Fish IBI1
EPT Index1
Normative Fish2
Fish Tissue Contamination3
- Carcinogens
- Mercury
Mine Drainage4
Acidic Deposition4
Total Nitrogen6
Total Phosphorus5

§
r-K
o'
ffi
t
§
8*
;>
en
S
1
Riparian Habitat
Instream Habitat
Watershed Condition1



Appalachians
31
27
31

10
4
14
11
4
5
24
25
25



43
24
36

12
8
24
24
2
1
31
10
27



ECOREGION
Ridge &

Western

WATERSHED
Alleghenv-
Blue Ridge Valley Appalachians Chesapeake Monongahela
14
14
19

5
0
0
8
1
1
5
28
10



31
36
40

16
0
0
2
15
3
34
28
35



30
37
19

7
6
24
0
24
20
28
38
31



23
20
31

5
4
3
11
7
2
12
25
21



31
22
46

19
14
20
26
2
2
28
6
36



Kanawha-
Upper Ohio
41
36
20

9
0
21
7
17
13
30
30
22



1 % stream miles in poor condition
2 % stream miles with normative fish
3 % stream miles with at least one constituent above
4 % stream miles affected

human health

carcinogen criteria or above


mammalian mercury

criteria





5 % stream miles with TP>100 Ug/L EPA guideline
6 % stream miles with IN > 3,
000 Ug/L, based on TP guideline
                                                                                                             STATE	

                                                                                                                   West
                                                                                                       Pennsylvania Virginia
                                                                                                           27
                                                                                                           27
                                                                                                           44

                                                                                                           15
                                                                                                            9
                                                                                                           16
                                                                                                           14
                                                                                                           21
                                                                                                           19
                                                                                                           38
44
25
26

 1
 0
13
14
 1
 5
26
18
                                                                                                                           "'••',
                                                                                                                           111


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            APPENDIX  C:  GLOSSARY
Acid Deposition: A complex chemical and
atmospheric phenomenon that occurs when
emissions of sulfur and nitrogen compounds
and other substances are transformed by
chemical processes in the atmosphere, often far
from the original sources, and then deposited on
earth in either a wet or dry form. The wet forms,
popularly called "acid rain," can fall as rain,
snow, or fog. The dry forms are acidic gases or
particulates.

Algae: Simple rootless plants that grow in
bodies of water (e.g., estuaries) at rates in
relative proportion to the amounts of nutrients
(e.g., nitrogen and phosphorus) available in
the water.

Anthropogenic: Originating from man, not
naturally occurring.

Assessment: Interpretation and evaluation of
scientific results for the purpose of answering
policy-relevant questions about ecological
resources, including (l)determinationofthe
fraction of the population that meets a socially
defined value and (2) association among
indicators of ecological condition and stressors.

Atmospheric Deposition: The flux (flow) of
chemicals and materials from the atmosphere
to the earth's surface. Depending on the
chemical or material, "dry" deposition (e.g., by
particles) can be less than, equal to, or greater
than "wet" deposition (e.g., precipitation).

Attribute: Any property, quality, or
characteristic of sampling unit. For example,
attributes of a tree, might include height and
leaf type. For fish, such atributes would be
size, feeding, or spawning habitat.
Base Flow: Sustained flow in a stream
primarily from a groundwater discharge.
Sometimes known as non-storm or dry
weather flow.

Benthos: Plants or animals that live in or on
the bottom of an aquatic environment such as
a stream.

Biological Assemblage: A grouping of
species from the same general category of
living organisms such as fish, aquatic insects,
hard wood trees, or riparian vegetation.

Biota: Living organisms including both plants
and animals found in a given area.

Buffer: A solution resistant to pH changes, or
whose chemical make up tends to neutralize
acids or bases without a change in pH.

Carcinogenic: Cancer causing

Channelization: The artificial enlargement,
straightening, or realignment of a stream
channel.

Community: The assemblage of populations
of plants and animals that interact with each
other and their environment. The community
is shaped by populations and their geographic
range, the types of areas they inhabit, species
diversity, species interactions, and the flow of
energy and nutrients through the community.

Competitor: An organism rivaling another
organism in the same area for food, habitat,
or other resource in limited supply.

Conductivity: A measure of the capacity of
water to conduct electricity. Conductivity
provides an indication of the concentration of
dissolved minerals in the water.
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  Detritus: Non-living organic matter (e.g.,
  dead organisms or leaves) in water.

  Ecology: The relationship of living things to
  one another and their environment, or the
  study of such relationships.

  Ecoregion: A relatively homogeneous
  geographic area perceived by simultaneously
  analyzing a combination of causal and
  integrative factors including land surface form,
  soils, land uses, and potential natural vegetation.

  Ecosystem: A natural unit formed by the
  interaction of a community of plants and
  animals with their environment (physical,
  chemical, and biological).

  Effluent: The discharge to a body of water
  from a defined or point source, generally
  consisting of a mixture of waste and water
  from industrial or municipal facilities.

  EMAP: Environmental Monitoring and
  Assessment Program - an EPA Office of
  Research and Development research program.

  Eutrophication: A condition in an aquatic
  ecosystem where high nutrient concentrations
  stimulate blooms of algae (e.g., phytoplankton).
  Algal decomposition may lower dissolved
  oxygen concentrations. Although eutrophication
  is a natural process in the aging of lakes and
  some estuaries, it can be accelerated by both
  point and nonpoint sources of nutrients.

  Food Web: An assemblage of organisms in
  an ecosystem, including plants, herbivores,
  and carnivores, which shows the relationship
  of who eats whom.
                                      Habitat: The place where a population or
                                      community (e.g., microorganisms, plants,
                                      animals) lives and its surroundings, both living
                                      and non-living.

                                      Headwater: The area that is the source or
                                      origin of a stream, above which no stream
                                      exists.

                                      Index: A summary of indicator scores.

                                      Invertebrates: Animals that lack a spinal
                                      column or backbone, including molluscs (e.g.,
                                      clams and oysters), crustaceans (e.g., crabs
                                      and shrimp), insects, starfish, jellyfish,
                                      sponges, and many types of worms that live
                                      in the benthos.

                                      Land Cover: Anything that exists on, and is
                                      visible from above, the earth's surface.
                                      Examples include vegetation, exposed or
                                      barren land, water, snow, and ice.

                                      Land Use: The way land is developed and
                                      used in terms of the kinds of anthropogenic
                                      activities that occur (e.g., agriculture, residential
                                      areas, industrial areas).

                                      Landscape: The set of traits, patterns,
                                      and structure of a specific geographic area,
                                      including its biological composition, its physical
                                      environment, and its anthropogenic patterns.
                                      An area where interacting ecosystems are
                                      grouped and repeated in similar form.

                                      Mammalian: Related to animals that are
                                      warm-blooded higher vertebrates that nourish
                                      their young with milk and have skin with hair

                                      Map Scale: A statement of a measure on the
                                      map and the equivalent measure on the earth,
                                      often expressed as a representative fraction
                                      of distance, such as 1:24,000.
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Mid-Atlantic Highlands Assessment

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Metric: A measurement or mathematical
function used to represent some property or
feature of living organisms. For example, the
number offish species intolerant of pollution
is one metric included in the fish Index of
Biotic Integrity.

Mine Tailings: Residue left from mining
coal, ores, or other material. These residues
can leach or contribute pollutants to streams.

Monitoring: The periodic or continuous
collection of data that is used to determine the
condition ecological resources.

Nonpoint Source: Refers to pollution that
enters water from dispersed and uncontrolled
sources, such as surface runoff, rather than
through pipes.

Nutrients: Essential chemicals (e.g., nitrogen
and phosphorus) needed by plants for
growth. Excessive amounts of nutrients can
lead to degradation of water quality (i.e.
eutrophication) by promoting excessive
growth, accumulation, and subsequent decay
of plants, especially algae (phytoplankton).

Order: A taxonomic unit in the scientific
classification for plants and animals, an order
is the unit in between family and class.

Organic Contaminants: Carbon containing
waste originating from domestic or industrial
sources contained in plant or animal matter

Parasite: An organism that lives off another
organism or host for survival and usually
injures the host.

Perturbation: A disturbance of motion,
course, arrangement or structure that creates
confusion.
Point Source: Refers to a source of
pollutants from a single point of conveyance,
such as a pipe. For example, the discharge
from a sewage treatment plant or factory is a
point source.

Population: A group of organisms that a
capable of interbreeding, which typically
represents a biological level of organization
equivalent to a species.

Predator: An animal that kills and consumes
other animals for its food.

ppb: Parts per billion equivalent to
micrograms per kilogram (ug/kg) or
micrograms per liter (ug/L).

ppm: Parts per million; equivalent to
micrograms per gram (ug/g) or milligrams per
liter (mg/L).

Sampling Methods: Procedures and practices
used to collect or measure physical, chemical or
biological material (e.g., temperature, water,
organisms) in or from the environment.

Scale: A distinctive relative size, extent or
degree of an area. For example, one scale of
measure or study might be an individual
stream while a larger scale of measure or
study might be a watershed that contains
many streams.

Sediment: Mud, sand, silt, clay, shell debris,
and other particles that settle on the bottom
of rivers, lakes, estuaries, and oceans.

Species: A group of individuals similar in
certain morphological and physiological
characteristics that are capable of
interbreeding and are reproductively
isolated from all other such groups.
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  Stream Reach: Portion of a stream; typically
  of a stream between the point where a stream
  enters (confluence) above to the point where
  a stream enters (confluence) below. Stream
  reaches can be a specific distance along a
  stream that was sampled for fish or aquatic
  insects.

  Stressor: Any physical, chemical, or
  biological entity that can cause or induce an
  adverse response.

  Surface Water: Water in streams,  lakes or
  estuaries that is visible on the surface of the
  earth. In contrast to groundwater, which is
  below the ground and not visible.

  Threatened, or Endangered: Living
  organisms placed in a special category for
  protection by the Endangered Species Act of
  1973.

  Toxic Substances (or material): Chemical
  compounds that are poisonous, carcinogenic,
  or otherwise directly harmful to plants and
  animals.

  Value: A characteristic of the environment
  that contributes to the quality of life of an
  area's inhabitants; for example, the ability
  of an area to provide desired functions such
  as food, clean water and air, aesthetic
  experience, recreation, and desired animal
  and plant species.
                                      Water Column: An imaginary cylinder of
                                      water from the water surface to the sediment
                                      that is used to describe the location of physical,
                                      chemical or biological properties or entities.

                                      Watershed: The entire area of land whose
                                      runoff of water, sediments, and dissolved
                                      materials (e.g., nutrients, contaminants) drain
                                      into a river, lake, estuary, or ocean.

                                      Wetlands: Lands transitional between
                                      terrestrial and aquatic systems where the
                                      water table is usually at or near the surface or
                                      where shallow water covers the land and
                                      where at least one of the following attributes
                                      holds: (1) at least periodically, the land
                                      supports aquatic plants predominantly;
                                      (2) undrained hydric soils are the predominant
                                      substrate; and (3) at some time during the
                                      growing season, the substrate is saturated with
                                      water or covered by shallow water (Cowardin
                                      et al. 1979). An area that is saturated by
                                      surface or ground water with vegetation
                                      adapted for life under those soil conditions.
                                      Examples of wetlands include swamps, bogs,
                                      fens, and marshes.

                                      Zooplankton: Very small, some even
                                      microscopic, animals that are suspended in
                                      the water and have very limited powers of
                                      moving against currents. These animals
                                      move primarily because the water carries
                                      or transports them.
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Mid-Atlantic Highlands Assessment

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