EPA/906/R-94/910 United Slates Region 6 July 1994 Environmental Protection Environmental Services Division Agency Dallas, Texas 75202-2733 Region 6 State/EPA *^ Ambient Toxicity Monitoring Program ------- U.S. EPA Region 6 State/EPA Ambient Toxicity Monitoring Program July 1994 Charlie Howell1, Terry Hollister2, & Abel Euresti2 Environmental Analysis Section Surveillance Branch Environmental Services Division U.S. Environmental Protection Agency, Region 6 1445 Ross Avenue Dallas. Texas 75202-2733 Inorganics Section Houston Branch Environmental Services Division U.S. Environmental Protection Agency, Region 6 10625 Fallstone Road Houston, Texas 77099 ------- Executive Summary Ambient toxicity testing, as a water quality assessment tool, complements chemical monitoring by allowing observation of the effects of multiple contaminants or contaminant sources on test organisms. Although biosurveys provide similar information, aquatic community impairment resulting from conventional pollutants or habitat degradation may mask the effects of toxicants. Laboratory toxicity testing also offers the ability to observe reproductive or teratogenic effects of contaminants that may not be apparent from a community-level biosurvey. The Region 6 State/EPA Ambient Toxicity Monitoring Program was established in 1990 to encourage States to integrate ambient toxicity testing into water quality assessments, to encourage States to acquire testing capabilities, to assess potential toxicity in Region 6 waterbodies, and to evaluate the effectiveness of implemented toxicity control measures. Regional staff encourage State and local agencies to participate in the program by collecting samples at routine monitoring sites. Samples are analyzed in the Region 6 Houston Laboratory using standard tests. Lab personnel forward results to the Region 6 Environmental Services Division and the collecting agency. Currently, over 1,050 tests have been conducted on samples collected from 144 stations. Regional staff recommend quarterly collection of water samples and biannual collection of sediment for a minimum of one year per station. Continued monitoring is recommended for waterbodies that exhibit toxic effects. The database also includes the results of one-time sample collections made during special studies. This report summarizes data collected to date and informs potential data users of the existence and capabilities of the program database. The report will serve to document the current database design and solicit ideas to maximize its utility. The validation of toxicity test results using in situ toxicity testing and the results of biosurveys for selected waterbodies is also discussed. About one-third of the stations sampled on multiple dates have exhibited recurrent toxic effects to at least one test species (Appendix B). This percentage is high, because investigators have targeted waterbodies with known or suspected water quality problems. Waterbodies that have exhibited recurrent toxicity are candidates for more intensive assessments, aimed at confirming the occurrence of toxic conditions or aquatic life use impairment, and determination of sources or causes of toxicity. The ambient toxicity monitoring program has provided a useful assessment tool for the identification of waterbodies with the potential for aquatic life impairment. Response from participating agencies suggests that interest in the program remains high. Requests for increased resources for testing and additional testing capabilities (bulk sediment & marine tests) have been received. Opportunities for aquiring these capabilities should be explored. Also, the Regional Environmental Services Division should assume an active role in assisting other agencies with special studies required to determine or document the sources of toxicity for waterbodies that have exhibited toxic effects. Additional copies or updates of this report may be obtained by contacting: Environmental Analysis Section (6E-SA) Environmental Protection Agency Region 6 1445 Ross Avenue Dallas, Texas 75202-2733 (214)665-8354 howell.charlie@epamail.epa.gov ------- Acknowledgments Willie Lane, Region 6 Environmental Services Division (ESD) Houston Branch, developed the original project plan and sampling protocols, coordinated site selection and sample collection among the agencies, and developed the original database. Philip Crocker, Region 6 Water Management Division, deserves recognition for the original monitoring program concept and initial development. Paul Koska, Region 6 ESD, helped to acquire station location data. Terry Hollister and Abel Euresti, EPA Houston Lab, deserve special recognition for their contributions to the program. While conducting ambient tests, they have exposed over 5,000 Ceriodaphnia dubia and 14,000 fathead minnows, and hatched over 150,000 young Ceriodaphnia dubia. Also, they have conducted all of the required statistical tests for data interpretation and associated chemical analyses. The following State personnel have participated either through program coordination or sample collection: Arkansas Department of Pollution Control and Ecology John Giese Indian Nations Council of Governments Richard Smith Louisiana Department of Environmental Quality Emelise Cormier, Sherri Courtney, John Kern New Mexico Environment Department David Tague, Larry Smolka Oklahoma Department of Environmental Quality Sylvia Rrtzsky, Jay Wright, Jimmie Pigg Oklahoma Water Resources Board Shon Simpson, Phil Moerschel, Main Hutcheson Oklahoma Conservation Commission Kendra Eddleman, Dan Butler Texas Natural Resources Conservation Commission Steve Twidwell, Christine Kolbe, Patrick Roques, Jeff Bertl, Wilson Snyder, Charlie Webster, Carlos Rubenstein, Sergio Mendez, Greg Larson, Bill Harrison, Suzanne Meyers, Jim Thompson, Linda Broach, Mark Luedke, Augustine de la Cruz, Jack Davis, Christine O'Keefe, Brenton Wade, Art Crow Texas Parks and Wildlife Department Dave Buzan, Tim Jurgensen We also thank Philip Crocker, Phillip Jennings, Wren Stenger, and Jack Ferguson, Region 6, for review and comment on the draft report. In addition, Stephen Twidwell and Cassy Shaukut, Texas Natural Resources Conservation Commission, conducted an extensive review of the report and data collected by the State of Texas. IV ------- Table of Contents Executive Summary iii Acknowledgments iv Introduction 1 Methods 1 Data Compilation 2 Results and Discussion 3 Potential for Development of Environmental Indicators 5 Conclusions & Recommendations 5 Literature Cited 7 Appendix A. Database Field Definitions 9 Appendix B. Waterbodies That Have Exhibited Toxic Effects 12 Appendix C. Ambient Toxic'rty Test Results and Laboratory Chemical Data 15 Table 1. Species & Test Methods Used in the Program 2 Figures 1. Database Structure 3 2. Samples in the Database 3 3. Species Used for Toxicity Tests 3 4. Relative Sensitivity of Ceriodaphnia dubia and Pimephales promelas 4 5. Linnville Bayou at CR 324 - Ceriodaphnia dubia Mean No. of Young per Female 6 6. Linnville Bayou at CR 324 - Pimephales promelas Test Effects 6 ------- Introduction Environmental Protection Agency (EPA) Region 6 has conducted an effluent toxicity testing program for over thirteen years. The Regional laboratory conducts standard tests by evaluating the response of organisms exposed to municipal and industrial effluents. Besides detection of effluent toxicity, results have been used to evaluate the sensitivity of test methods, and the relative toxicity of Standard Industrial Categories (Crocker, ej aj. 1989). Although useful from a regulatory standpoint, effluent testing is of limited value for water quality assessment, because of the inability to evaluate potential synergistic, antagonistic, or additive effects of multiple contaminant sources. Investigators have documented the ability to predict aquatic community response based on results of effluent testing in conjunction with ambient toxicity testing (Bastian ej aj., 1990; Birge ej aj., 1989; and Mount ej a]., 1985a-b and 1986a-d). Ambient toxicity testing, as a water quality assessment tool, complements chemical monitoring with the direct assessment of the compound physiological effects of multiple contaminants or contaminant sources on test species. Although biosurveys provide similar information, aquatic community impairment resulting from conventional pollutants or habitat limitations may mask potential effects of toxicants. Laboratory toxicity testing also offers the ability to observe reproductive or teratogenic effects of contaminants that may not be apparent from a community-level biosurvey. In 1990, the Region 6 State/EPA Ambient Toxicity Testing Program was initiated to establish a system for the assessment of ambient toxicity. The program was developed to encourage State agencies to integrate ambient toxicity testing into water quality assessments, to encourage States to acquire testing capabilities, to assess potential toxicity in Region 6 waterbodies, and to evaluate the effectiveness of implemented toxicity control measures. Regional staff encourage State and local agencies to participate in the program by collecting samples at monitoring sites. Samples are tested by the Region 6 Houston Laboratory. Lab personnel forward results to the Region 6 Environmental Services Division and the collecting agency. Region 6 States have participated in the program to varying degrees. Oklahoma and Texas agencies have collected water samples quarterly from selected sites. The New Mexico Environment Department has collected samples during intensive surveys. The Arkansas Department of Pollution Control and Ecology and the Louisiana Department of Environmental Quality have collected samples for a special study on the Mississippi River. Currently, over 1,040 tests have been conducted on samples from about 144 stations. Data from 180 tests conducted during a special study on the Rio Grande are unavailable for public review, pending completion of the final study report. These results were included in summary statistics, but are not included in data tables. This report will summarize data collected to date and inform potential data users of the existence and capabilities of the database. Also, the report investigates potential use of the data for environmental indicators, and encourages continued participation in the program. The report will serve to document the current database design and solicit ideas to maximize its utility. Methods Regional staff recommend quarterly collection of ambient water and biannual collection of sediment for a minimum of one year to increase the probability that intermittent toxicity will be detected. Continued monitoring is recommended for waterbodies that have exhibited toxic effects. The database also includes the results of one-time collections made during intensive surveys or special studies. Grab samples of ambient water are collected on a single day, in sufficient volume (two gallons) to provide for renewal of test water throughout the test period. This approach is inconsistent with guidance (Weber ej aj., 1989), developed primarily for regulatory application, which suggests daily sampling or a minimum of three samples collected every other day. The decision to collect a single sample for testing is based on consideration of personnel and travel costs for renewal sample collection. Samples are generally collected during other ambient monitoring, and require no additional travel. This approach has proven effective for the primary objective of this program, i.e., screening for potential ambient toxicity. ------- Water samples are collected from the main flow of the stream in a clean polyethylene container(s) rinsed with ambient water before filling. Sediment samples are composited from a minimum of three subsamples of the upper layer of recently deposited, unconsolidated sediment. Subsamples are composited and mixed before being transferred to a clean glass or polyethylene containers). Collecting equipment is thoroughly cleaned between sites and rinsed with ambient water before use. Upon collection, water and sediment samples are placed in ice for shipment to the laboratory. In the laboratory, sediment elutriates are prepared by combining a subsample from the homogenized sediment sample with appropriate culture water. The sediment and water are combined in a sediment-to- water ratio of 1:4 by volumetric displacement. After combining, the mixture is tumbled end-over-end for approximately 24 hours at 3 - 4 °C. After settling, the elutriate is siphoned off and filtered through a 1.5 micron glass fiber filter. Standard laboratory tests and statistical data analyses are conducted according to Short-term Methods for Estimating the Chronic Toxic'rtv of Effluents and Receiving Waters to Freshwater Organisms (Weber, et a]., 1989), Short-Term Methods for Estimating the Chronic Toxicitv of Effluents and Receiving Waters to Marine and Estuarine Organisms (Weber, et a]., 1988), and Methods for Measuring the Acute Toxic'rty of Effluents to Freshwater and Marine Organisms (U.S. EPA, 1991). Test species and methods are described in Table 1. Table 1. Species & Test Methods Used In the Program Ceriodaphnia dubia seven-day survival and reproduction test; short-term chronic test observing mortality rate and the number of young produced per female. Daphnia pulex 48-hour survival test; acute test observing mortality rate. Pimephales promelas (Fathead Minnow) seven-day embryo-larval survival and teratogenicity test; short-term chronic test observing mortality rate, teratogenic effects, and abnormal swimming behavior. Pimephales promelas seven-day larval survival and growth test; short-term chronic test observing mortality rate and dry weight at test termination. Cyprinodon variegatus (Sheepshead Minnow) nine-day embryo-larval survival and teratogenicity test; short-term chronic test observing mortality rate, teratogenic effects, and abnormal swimming behavior (used for estuarine or saline waters). Data Compilation Regional staff enter and maintain test data in a Microsoft Access for Windows™ relational database. Three data tables contain (1) station location details, (2) toxicity test conditions and results, and (3) chemical test results (Figure 1 & Appendix A). State station numbers and sample identification numbers are key fields that link the tables. The software forces maintenance of referential integrity between the tables to preclude test data entry where supporting data is nonexistent. Data entry forms contain validation checks that prevent entry of erroneous numeric values. The Windows™ environment provides remarkable advantages for data entry with the ability to copy and later paste key field data to and from memory, eliminating repetitive data entry. ------- The data tables may be exported for use with other relational database software, after reestablishing links. Also, some or all of the data fields may be exported in a single table for use with spreadsheet programs. A backup copy of the database is made after each data entry session. Annually, the database will be exported to labeled diskettes in an ASCII format and maintained by the Environmental Analysis Section. Hardcopies of test results will also be stored in the Houston laboratory. Figure 1. Database Structure Results and Discussion About one-third of the stations sampled on multiple dates have exhibited recurrent toxic effects to at least one test species (Appendix B). This percentage is high, because investigators have targeted waterbodies with known or suspected water quality problems. Waterbodies that have exhibited recurrent toxJcity are candidates for more intensive assessments, aimed at confirming the occurrence of toxic conditions or aquatic life use impairment. Priority for investigation should be based on the frequency and degree of observed toxicity as well as other important factors, such as waterbody designated uses, actual uses, economic importance, endangered species, and known or suspected causes of impairment. Initially, Oklahoma agencies were the only major source of sediment samples, as other agencies opted to collect only water samples. Therefore, sediment data in the database is limited. With regard to test species, Ceriodaphnia dubia and Pimephales promelas (Fathead Minnow) have been used routinely. The Daphnia pulex acute test was in use when the program was initiated. Cyprinodon variegatus (Sheepshead Minnow) has been used for some saline or estuarine waters. Figures 2. & 3. show the relative breakdown of approximately 1,040 tests for sample types and test species. Figure 2. Samples in the Database (n=1047) Water Figure 3. Species Used for Testing Coriodaphnle <**ta^- Sadlment Cyprinodon varieyatus Pimephales promelas The database offers an opportunity to further examine the relative sensitivity of selected toxicity test methods. For Ceriodaphnia dubia and Pimephales promelas, 340 pairs of side-by-side tests have been conducted on ambient water samples. Ninety-seven pairs of side-by-side tests were conducted on sediment samples using these species. Twenty-eight (28%) percent of the water samples, and thirty (30%) percent of the sediment elutriates, caused significant toxic effects to at least one test species. Graphs in Figure 4. are based on the seventy-six water samples and nineteen sediment samples that caused toxic effects to either one of the two species. The results strongly suggest that Ceriodaphnia dubia is a more sensitive test species, although this comparison may be biased by specific causes of toxicity in the assessed waterbodies. The results of Pimephales promelas tests may be more important as an indicator of potential causes of toxicity, rather than as an indicator of potential toxic effects, since this species indicated toxicity only six times when Ceriodaphnia dubia did not. Similar comparisons using Daphnia pulex or Cyprinodon variegatus were not made, because of the limited amount of data for these species. ------- Figure 4. Relative Sensitivity of Ceriodaphnia dubia and Pimephales promelas - Results from Side-by-Side Testing of Samples or Elutriates that Caused Toxicity to Either Species Species Indicating Significant Toxicity Water Samples (n=96) Sediment Samples (n=27) Both Species ^^H Pimephales promelaa jggfr Ceriodaphnia dubia (Most Sensitive) Although data for side-by-side sediment elutriate and water testing is limited, results confirm the importance of collecting both samples. While sediment samples are more difficult to collect, sediment elutriates yielded positive toxicity results more frequently than water samples. These findings are consistent with observations made by Bastian et aj. (1989). The toxicity screening procedures, as described, have important limitations. The collection of single grab samples allows the possibility that intermittent toxicity may not be detected. The sediment elutriation procedure will extract only those contaminants that are water-soluble or loosely bound to the sediment (Nriagu and Lakshminarayana, 1989). The fact that any given station has not tested positively, does not rule out the possibility that water quality or sediment quality problems exist. During an assessment of the Guadalupe River near Victoria, Texas, Trebatoski (1991) used both laboratory and in situ toxicity tests. The study was designed to investigate the potential impact of a domestic wastewater treatment facility on the river and to examine the utility of ambient toxicity testing. Caged fathead minnows were exposed to the effluent and the river, at an upstream site and two downstream sites, for a 48-hour period. Both the in situ and laboratory tests demonstrated effluent toxicity. The test results differed in that acute effects were observed in the field (100% mortality after six hours), whereas, chronic effects were observed in the laboratory (55% mortality after eight days). Results of in situ and laboratory tests were consistent regarding instream conditions, as neither test demonstrated significant toxicity at upstream nor downstream sites. It should be noted that the Pimephales promelas embryo-larval survival and teratogenicity test routinely used in the ambient toxicity monitoring program differs from the larval survival and growth test required of National Pollutant Discharge Elimination System (NPDES) permittees. The embryo-larval test was selected as a more cost-effective initial screening test. When a NPDES permittee is suspected as a source of toxicity, the larval survival and growth test should be conducted. Also, investigators should make return trips to the srte(s) to collect samples for renewal of test water. The ambient toxicity monitoring program sampling protocols will be revised in the future to include these recommendations. ------- Potential for Development of Environmental Indicators Ambient water and sediment quality tests may serve as environmental indicators if comparisons of results are made over time for given stations. Because additions and deletions of stations may occur, program data should not be used collectively as an environmental quality indicator. For example, an agency may opt to temporarily discontinue monitoring of waterbodies that frequently exhibit toxicity while corrective actions are implemented. This may result in the appearance of improving conditions, if alternate stations do not exhibit toxicity. Conversely, the selection of several new stations that continually exhibit toxicity may produce the appearance of deteriorating conditions. Variability in meteorological conditions may also cause temporary declines or increases in the occurrence of ambient toxicity resulting from nonpoint sources. The data may be useful for regional environmental indicators, over a period of many years, using a consistently monitored subset of stations. On a waterbody or station-specific basis, data will be useful as an environmental indicator in shorter terms. For example, the installation of dechlorination or nitrification facilities at a specific treatment facility, or the implementation of best management practices to control nonpoint contaminant sources may result in an immediate reduction or elimination of the occurrence of positive toxicity tests. Kolbe (1994) presents an example of how ambient toxicity data are applied as an environmental indicator for a specific waterbody. The Phillips 66 Refinery and Petrochemical Complex located in Brazoria County, . Texas, discharges treated wastewaters to LJnnville Bayou. The discharge contributes almost all instream flow during dry weather. Phillips 66, the only permitted point source on the bayou, discharges an average of four-million gallons per day. Previous studies associated with this discharge have documented impacts such as oily sheens, elevated pH, low dissolved oxygen, and high ammonia and sulfide concentrations. Severe impacts to fish communities of LJnnville Bayou have been observed during several studies. During the most recent survey in August 1992, investigators collected nine fish species at an upstream site; however, no fish could be collected 0.4 miles downstream from the discharge (mosquitofish, Gambusia affinis, were observed in the shallows at the downstream site). Only five fish species were collected nine miles downstream, and mosquitofish comprised 97% of the individuals in this sample. Ambient toxicity testing allowed investigators to identify toxicity as a probable contributing factor to observed fish community impairment. Effluent toxicity at this facility was attributed to chlorides, ammonia, and one or more organic toxicants. Phillips 66 has completed several treatment plant upgrades in attempts to achieve compliance with permit conditions. The most significant improvements resulted from the identification and removal of ammonia sources in September 1992 and the startup of activated sludge and powdered activated carbon treament systems in April 1993. Recent ambient toxicity test results suggest a reduction in the occurrence of ambient toxicity that appears to coincide with the removal of ammonia sources (Figures 5. and 6.) Historically, water samples collected from LJnnville Bayou at Hasema Road (Station No. 1300.0726), located immediately downstream from the discharge, caused toxic effects to both Ceriodaphnia dubia and Pimephales promelas. Recent tests have shown no significant effects to test organisms exposed to ambient water collected from this site. Follow-up studies are being conducted to monitor the compliance status of the facility and future trends in the condition of aquatic communities of LJnnville Bayou. Conclusions & Recommendations The ambient toxicity monitoring program has proven useful for identifying waterbodies with the potential for aquatic life impairment. Although data for side-by-side laboratory toxicity tests and in situ testing is limited, results indicate that toxicity testing is a reliable indicator of instream conditions where habitat quality or conventional pollutants are not limiting. About one-third of the stations sampled on multiple dates have exhibited recurrent toxic effects to at least one test species. The frequency of observed toxicity is high, because investigators have generally targeted waterbodies with known or suspected water quality ------- Fig. 5 LJhnvilto Bayou at Hasema Rd (CR 324) Ceriodaphnladubla Moan No. of Young par Femato 10 o § Control Sample ** ** I** 100 w 20 *W 7JW 1(»0 «M «M 7*i 10*11M1 . Data • Significant affect (PXI.85) ** No young produced ki uinple due to mortaMy- 7/K M2 121*2 M* «W 12W Fig. 6 LlnnvHe Bayou at Haaema Rd (CR 324) Pimephatos pmmdas Test Effects * Control Sample Mm MM 4NO 4m 7/01 10m 12«1 3192 7H2 6102. 1202 3*3 OK3 12/93 •StaneTcant effect (P>0.85) Taet eftecto Include mortally, taratoganldty, or abnormal iwknmtag behavior. problems. Waterbodies that have exhibited recurrent toxicity are candidates for more intensive assessments, aimed at confirming the occurrence of toxic conditions or aquatic life use impairment, and determination of sources or causes of toxicity. Effluent testing may be conducted where point sources are suspected as the primary cause of instream toxicity. Toxicity identification evaluations (TIEs), chemical analyses, and/or biological assessments may be useful for documenting the causes and instream effects of observed toxicity. States should continue to monitor those waterbodies exhibiting toxicity until successful corrective actions are completed. It may be prudent to temporarily discontinue monitoring at some stations to allow other waterbodies to be screened. The Regional Environmental Services Division should assume an active role in assisting other agencies with special studies required to determine or document the sources of toxicity for waterbodies that have exhibited toxic effects. For some waterbodies, such studies have been conducted or are currently underway. The information from these studies should be compiled for inclusion in the database. Response from participating agencies suggests that interest in the program remains high. Requests for increased resources for testing and additional testing capabilities (bulk sediment & marine tests) have been received. Opportunities for acquiring these capabilities should be explored. The utility of the Pimephales promotes chronic test should be reviewed in future data analyses and reports. Data suggest that the test generally lacks the sensitivity to detect toxic conditions that are not demonstrated by the Ceriodaphnia dubia test. The results of Pimephales promelas toxicity tests may be more important as an indicator of potential causes of toxicity, rather than as an indicator of potential toxic effects. Many other data sources exist which could augment the database. An active program of data acquisition is recommended. This will facilitate use of the data and prevent potential data loss. Efforts should also be directed toward database modifications necessary to make the data more accessible to potential users. 6 ------- References Bastian, M., W. Lane, P. Koska, C. Young, T. Hollister, B. Trebatoski, L Smolka, and D. Butler. 1989. U.S. EPA Region 6 Ambient Toxicity Testing Demonstration Projects: Evaluation of Results. U.S. Environmental Protection Agency, Region 6, Dallas, Texas. Birge, W.J., J A Black, T.M. Short, and A.G. Westerman. 1989. A Comparative Ecological and Toxicological Investigation of a Secondary Wastewater Treatment Plant Effluent and its Receiving Stream. Environmental Toxicology and Chemistry. 8:437-450. Crocker, PA, TA Hollister, A. Euresti, and D. Neleigh. 1989. U.S. EPA Region 6 Effluent Toxicity Testing Program: Evaluation of Results. U.S. Environmental Protection Agency, Region 6, Dallas, Texas. Greene, J.C., C.L. Bartels, W.J. Warren-Hicks, B.R. Parkhurst, G.L. Under, SA Peterson, and W.E. Miller. 1988. Protocols for Short-Term Toxicity Screening of Hazardous Waste Sites. Environmental Research Laboratory, U.S. Environmental Protection Agency, Corvallis, Oregon, EPA/600/3-88/029. Kolbe, C.M. 1994. Effects of Petroleum Refinery Wastewater on Water Quality in LJnnville Bayou (Brazoria/Matagorda County, Texas). Texas Natural Resources Conservation Commission Region 12, Houston, Texas. Report AS-16/SR. Mount, D.I., A.E. Steen, T.J. Norberg-King. 1985a. Validity of Effluent and Ambient Toxicity Testing for Predicting Biological Impact on Five Mile Creek, Birmingham, Alabama. U.S. Environmental Protection Agency, Environmental Research Laboratory, Duluth, Minnesota, EPA/600/8-85/015. Mount, D.I., and T.J. Norberg-King. 1985b. Validity of Effluent and Ambient Toxicity Testing for Predicting Biological Impact, Scippo Creek, Circleville, Ohio. U.S. Environmental Protection Agency, Environmental Research Laboratory, Duluth, Minnesota, EPA/600/3-85/044. Mount, D.I., and T.J. Norberg-King. 1986a. Validity of Effluent and Ambient Toxicity Testing for Predicting Biological Impact, Kanawha River, Charleston, West Virginia. U.S. Environmental Protection Agency, Environmental Research Laboratory, Duluth, Minnesota, EPA/600/3-86/006. Mount, D.I., A.E. Steen, T.J. Norberg-King. 1986b. Validity of Effluent and Ambient Toxicity Testing for Predicting Biological Impact, Ohio River, Near Wheeling, West Virginia. U.S. Environmental Protection Agency, Environmental Research Laboratory, Duluth, Minnesota, EPA/600/3-85/071. Mount, D.I., A.E. Steen, T.J. Norberg-King. 1986c. Validity of Effluent and Ambient Toxicity Testing for Predicting Biological Impact, Back River, Baltimore Harbor, Maryland. U.S. Environmental Protection Agency, Environmental Research Laboratory, Duluth, Minnesota, EPA/600/8-86/001. Mount, D.I., A.E. Steen, T.J. Norberg-King. 1986d. Validity of Effluent and Ambient Toxicity Testing for Predicting Biological Impact, Naugatuck River, Waterbury, Connecticut. U.S. Environmental Protection Agency, Environmental Research Laboratory, Duluth, Minnesota, EPA/600/8-86/005. Nriagu, J.O., and J.S.S. Lakshminarayana. 1989. Aquatic Toxicology and Water Quality Management. John Wiley and Sons, Inc. New York, New York. Trebatoski, B. 1991. Guadalupe River Toxicity Assessment. Texas Natural Resources Conservation Commission. Austin, Texas. Report No. LP 91-04. U.S. EPA. 1991. Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms. U.S. Environmental Protection Agency, Office of Research and Development, Washington, D.C., EPA/600/4-90/027. ------- References, cent. Weber, C.I., W.H. Peltier, T.J. Norberg-King, W.B. Homing II, FA Kessler, J.R. Menkedick, T.W. Neiheisel, PA Lewis, D.J. Klemm, Q.H. Pickering, E.L Robinson, J.M. Lazorchak, L.J. Wymer, and R.W. Freeburg. 1989. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms. U.S. Environmental Protection Agency, Environmental Research Laboratory, Cincinnati, Ohio, EPA/600/4-89/001. Weber, C.I., W.B. Horning II, D.J. Klemm, T.W. Neiheisel, PA Lewis, E.L. Robinson, J. Menkedick, and F. Kessler. 1988. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Marine and Estuarine Organisms. U.S. Environmental Protection Agency, Environmental Research Laboratory, Cincinnati, Ohio, EPA/600/4-87/028. ------- Appendix A. Database Field Definitions Text in parentheses indicates the type of database field. Station Details - tbIStations Agency (Text) - State or local agency responsible for sample collection, identified by abbreviation or acronym. County (Text) - county or parish of sampling location, identified by full name. Description of Waterbody (Text) - narrative description of sampling station location, including major highway crossings, references to other major landmarks, or identification of the nearest city. EPA Reach Number (Number) - EPA River Reach identified by agency number. Latitude (Number) - latitude of sampling point. Longitude (Number) - longitude of sampling point. Notes Specific to Station (Memo) - memo field used to store information specific to station, such as suspected sources of toxic contaminants and references for special study reports. State (Text) - State where sample collected, identified by two-letter abbreviation. Station Number (Text) - permanent station identifier, assigned by State or local agency. Links station details table with toxicity test results table. Toxicity Test Conditions & Results - tblResuKs Sample ID (Text) - number or identifier assigned to water or sediment sample by laboratory. Links toxicity test conditions table with chemical results table. Test Date (Date) - date test initiated. Sample Type (Text) - identifies sample matrix, as either water (W) or sediment (S). Test Species (Text) - identifies the organism used during the test, as Daphnia pulex (D), Cen'odaphnia dubia (C), Pimephales promelas (P), or Cyprinodon variegatus (S). Test Duration (Number) - duration of test in days. Test Type (Text) - identifies test as acute (A) or chronic (C). Significant Mortality/Effects (Yes/No) - indicates whether or not differences in mortality or other test effects between the control and sample were statistically significant. Significant Difference in Young per Female (Yes/No) - indicates whether or not differences in number of young produced per female in the control and sample were statistically significant. Significant Difference in Weight (Yes/No) - indicates whether or not differences in dry weight of organisms between the control and sample were statistically significant (fish tests). 9 ------- Sample Effects/Mortality (Number) - percentage of test organisms affected during test. In addition to mortality, effects may include organisms exhibiting terata, or abnormal swimming behaviour. Control Effects/Mortality (Number) - percentage of control organisms affected during test. In addition to mortality, effects may include organisms exhibiting terata, or abnormal swimming behaviour. Sample Young per Female (Number) - mean number of young produced per female in sample or elutriate during Ceriodaphnia dubia survival and reproduction test. Control Young per Female (Number) - mean number of young per female produced in control during Ceriodaphnia dubia survival and reproduction test. Sample Weight (Number) - mean dry weight offish exposed to sample or elutriate, upon completion of test. Control Weight (Number) - mean dry weight offish exposed to control, upon completion of test. Notes Specific to Test (Memo) - memo field used to store observations specific to test or sample collection. Chemical Results - tbIChemicalResults . Alkalinity (Number) - alkalinity of water sample or sediment elutriate, measured in the lab and expressed in mg/L. Conductivity (Number) - conductivity of water sample or sediment elutriate, measured in the lab and expressed in umhos/cm. Hardness (Number) - hardness of water sample or sediment elutriate, measured in the lab and expressed in mg/L. Laboratory (Text) - laboratory identifier. Currently, the EPA Region 6 Houston lab is the only contributor to the database. Nondetect Cl (Text) - denotes total chlorine concentration below detection limit. Nondetect Ammonia-N (Text) - denotes ammonia-N concentration below detection limit. Nondetect Unionized Ammonia-N (Text) - denotes unionized ammonia-N concentration below detection limit. pH (Number) - pH of water sample or sediment elutriate, measured in the lab and expressed in standard pH units. Salinity (Number) - salinity of water sample or sediment elutriate, measured in the lab and expressed in mg/L. Sample Collection Date (Date) - date of sample collection. Total Ammonia-N (Number) - total ammonia-N concentration of water sample or sediment elutriate, measured in the lab and expressed in mg/L. Unionized Ammonia-N (Number) - unionized ammonia-N concentration of water sample or sediment 10 ------- elutriate, measured in the lab and expressed in mg/L. Total Chlorine (Number) - total chlorine concentration in water sample or sediment elutriate, measured in the lab and expressed in mg/L. 11 ------- Appendix B. Waterbodies That Have Exhibited Significant Toxic Effects in One or More Tests Grouped by State and Listed by Station Number Station State Number Arkansas 1 2 Louisiana S-52 S-54 New Mexico UPR21 1.001 537 UPR21 1.001 539 Oklahoma OB002 20B 7 OCC2 OCC5 OCC7 ODEQ10 ODEQ12 ODEQ2 ODEQ3 Abbreviated Description Mississippi River near Elaine Mississippi River near Arkansas City Mississippi River across from Bell Chasse Mississippi River across from Plaquemine Spring Arroyo at Airport Road Gallinas River at Grand Bird Creek in Tulsa Tar Creek in Miami Tar Creek at State Line North of Miami Gaines Creek near Lake Eufala Pitt Creek East of Hartshome Spring Creek East of Edmond Acid Creek Tributary of Chickaskia River near Blackwell Cimarron River N. of Lone Mountain Coal Creek near Henryetta No of Samples Causing Toxic Effects Sample Test Primary Secondary Type1 Species1 Endpoint3 Endpoint4 W W W W W W W W W W W W W S W S S W W W S W W W S S W S S S W W c c c c p c p c c c p p c c c c p c p c c p c p c p S c D p c p 0 0 0 0 0 1 1 1 0 3 2 2 3 1 1 2 2 2 2 2 0 1 3 1 1 1 11 2 2 4 7 6 1 1 2 1 1 0 0 1 1 0 0 0 0 2 1 0 0 0 0 1 1 0 1 0 1 0 0 0 0 0 0 0 Total No. of Samples 6 6 10 12 12 1 1 1 3 3 2 2 3 2 6 2 2 2 2 2 2 4 3 3 2 2 11 2 2 4 8 7 'Sample - Water (W) or Sediment (S) 'Species- Ceriodaphnia dubia (C). Pimephales promelas (P), Daphniapulex(D). Cyprinodon variegatus (S) •Primary Endpoints - dependent on test species, i.e., mortality for crustacean tests; mortality, teratogenicity, or abnormal swimming behavior for fish tests. 'Secondary Endpoints - dependent on test species, i.e., significant difference in number of young per female for Ceriodaphnia dubia: difference in weight for fish larval survival and growth tests (absence of secondary endpoint effects may be due to mortality). 12 ------- No of Samples Gaming Toxic Effect* Station State Number OOEQ4 ODEQ6 ODEQ7 ODEQ9 Texas 0214.0160 0304.0100 0601.0800 0700.0200 0805.0300 0841.0500 0902.0100 1006.0200 1007.9200 1113.0020 1200.7820 1200.7822 1200.7830 1200.7832 1200.7834 1218.0270 1300.0710 Abbreviated Description Lake Francis at Upper End Bitter Creek Tributary of San Bote Creek Tributary of Eliza Creek Wichita River at End of Eastland Lane Days Creek at South of Texarkana Neches River Near Beaumont Alligator Bayou in Port Arthur Trinity River below Dallas W. Fork Trinity River in Grand Prairie Cedar Bayou Southwest of Dayton Houston Ship Channel at Greens Bayou Vince Bayou at Houston Ship Channel Armand Bayou Tidal Upstream of NASA Bryan Municipal Lake near Dam Bryan Municipal Lake Mainbody Finfeather Lake near Dam Finfeather Lake Main Body Finfeather Lake Headwater Nolan Cr. Above Bell County WCID Outfall Linnville Bayou Upstream from Caney Creek Sample Test Type1 Species' W W W W S S S W W W S S W S S W W W S W W W S S W S W S W W W W W W W c c p c c p c c p c p c c c p c p c c c p c S S c c c c c p c c c c p Primary Secondary Endpoinr Endpoint' 0 1 3 3 2 1 2 4 1 0 1 1 0 2 2 2 2 0 0 1 1 0 2 1 0 1 0 1 2 1 1 1 1 0 1 1 0 0 0 0 0 0 0 0 1 0 1 1 0 0 1 0 1 1 0 0 1 0 0 1 1 1 0 0 0 0 0 2 1 0 Total No. of Samples 2 3 4 4 2 1 2 4 4 2 2 2 6 3 3 5 5 5 3 6 5 1 4 3 5 1 6 1 10 10 2 1 8 1 1 'Sample - Water (W) or Sediment (S) 'Species- Cortodaphniadubia (C), Pimephalespromdas(P), Daphnlapufox(D), Cyprinodon variegatus (S) 'Primary Endpoints - dependent on test species, i.e., mortality for crustacean tests; mortality, teratogenteity, or abnormal swimming behavior for fish tests. ^Secondary Endpoints - dependent on test species, i.e., significant difference in number of young per female for Ceriodaphnia dubia: difference in weight for fish larval survival and growth tests (absence of secondary endpoint effects may be due to mortality). 13 ------- No of Samples Causing Toxic Effects Station State Number 1300.0717 1300.0726 1302.0100 1412.0140 1421.0470 1429.0800 1429.TMP1 1906.0230 1906.0240 2202.0600 2304.0075 2304.0150 2306.0300 2308.0050 Abbreviated Description Linnville Bayou at Boone Road Linnville Bayou at Hasema Road San Bernard River Northeast of Boling Colorado River in Colorado City North Concho River in San Angelo Town Lake at Longhom Dam Waller Creek at 2nd Street in Austin Leon Creek South of San Antonio Leon Creek Below Kelly AFB Arroyo Colorado South of Weslaco Rio Grande Below Laredo Rio Grande near US 277 Bridge in Eagle Pass Rio Grande below Rio Conchos Confluence Rio Grande at Zaragosa International Bridge Sample Test Type1 Species2 W W S W W W W S W W W W W W W S W W W W S c p p c p c c c c c c c p c c c c c p c c Primary Secondary Endpoint3 Endpoint* 0 1 1 5 9 0 1 0 1 1 0 1 1 1 2 1 0 0 1 1 0 1 0 0 9 0 1 1 1 0 2 1 3 0 1 3 0 1 2 0 1 1 Total No. of Samples 2 3 3 16 13 6 2 2 6 4 1 9 9 1 6 3 9 7 7 6 3 1Sample - Water (W) or Sediment (S) 'Species- Ceriodaphniadubia (C), Pimephalespromelas(P), Daphniapulex(D), Cyprinodon variegatus (S) 'Primary Endpoints - dependent on test species, i.e., mortality for crustacean tests; mortality, teratogenicity, or abnormal swimming behavior for fish tests. 'Secondary Endpoints - dependent on test species, i.e., significant difference in number of young per female for Ceriodaphnia dubia: difference in weight for fish larval survival and growth tests (absence of secondary endpoint effects may be due to mortality). 14 ------- ------- Appendix C. Ambient Toxicity Test Results and Laboratory Chemical Data (Grouped by State and Agency) Station Humber Waterbody Description Test Sample Test Percent Date of Collection Type Species Effected Control Percent significant Test Effected Effects YPF Test Control Significant Test Control Significant Weight Weight Dtfln Duration pH YPF DiflnYPF (mg) (mg) Weight (Days) (S.U.) 1 Mississippi River at McCalllster Grain near Elaine 14-May-91 W 14-May-91 W 16-Jul-91 W 16-Jul-91 W 10-Sep-91 W 10-Sep-91 W 19-Nov-91 W 19-Nov-91 W 07-Jan-92 W 07-Jan-92 W 11-Mar-92 W 11-Mar-92 W C 0 P 7 C 10 P 7 C 0 P 7 C 10 P 0 C 0 P 7 C 10 P 3 0 ( 10 ( 0 ( 10 ( 0 ( 7 ( 10 ( 0 ( 0 ( 7 ( 0 ( 3 ( 3 " 3 3 I6'3 3 3 18-1 3 3 13-3 3 3 io-i 3 3 " 3 16.2 O 15.9 O 16.4 O 13.7 O 12.2 (§) 16.9 O 0.595 0.537 0.564 0.493 0.777 0.844 0.657 0.64 0.336 0.273 0.437 0.469 7 O 7 7 O 7 7 O 7 7 O 7 7 O 7 7 O 7 7.2 7.2 7.7 7.7 8.0 8.0 8.1 8.1 7.4 7.4 6.8 6.8 2 Mississippi River at McCallle Access near Arkansas City 14-May-91 W 14-May-91 W 16-Jul-91 W 16-Jul-91 W 10-Sep-91 W 10-Sep-91 W 19-Nov-91 W 19-Nov-91 W 07-Jan-92 W 07-Jan-92 W 11-Mar-92 W 11-Mar-92 W C 0 P 0 C 10 P 3 C 0 P 3 C 10 P 0 C 10 P 3 C 0 P 7 0 ( 10 ( 0 ( 10 ( 0 ( 7 ( 10 ( 0 ( 0 ( 7 ( 0 ( 3 ( 3 ".1 3 3 16 3 3 17 3 3 12-6 3 3 1l>-2 3 3 17.6 3 16.2 O 15.9 O 16.4 O 13.7 O 12.2 (§) 16.9 O 0.54 0.537 0.534 0.493 0.773 0.844 0.658 0.64 0.297 0.273 0.422 0.469 7 O 7 7 O 7 7 O 7 7 0 7 7 O 7 7 0 7 6.9 6.9 7.3 7.3 7.5 7.5 8.1 8.1 6.5 6.5 6.5 6.5 S-31 9 Mississippi River Midstream at Plaquemlne Ferry Landing East ofPlaquemlne 14-May-91 W 14-May-91 W 11-Jun-91 W 11-Jun-91 W Sample Types Test Species C 0 P 0 C 0 P 3 0 ( 10 ( 0 ( 0 ( 3 I4-7 3 3 15-8 3 16.2 O 16.8 O 0.562 0.537 0.677 0.695 7 O 7 7 O 7 7.2 7.2 7.9 7.9 Agency EPA Reach Latitude Longitude County State Total Total Unionized Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-N (mg/l) (mg/L) (umhos/cm) (mg/L) (mg/L) (mgfl) ADPCE 102 102 132 132 130 130 120 120 88 88 154 154 ADPCE 94 94 139 139 130 130 141 141 67 67 97 97 LDEQ 84 84 92 92 156 202 156 202 183 401 183 401 164 372 164 372 130 393 130 393 51 189 51 189 127 254 127 254 08030202002 124 205 124 205 188 411 188 411 172 358 172 358 162 445 162 445 35 141 35 141 134 300 134 300 08070100007 112 266 112 266 128 282 128 282 34'22W 90*45'55" < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 33«39-20- 90*1 2'43- < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 SOMTW 91'12'Sr < 0.1 < 0.1 < 0.1 < 0.1 Phillips AR 0.6 0.6 0.1 0.1 0.7 0.7 0.2 < 0.1 0.2 < 0.1 0.4 < 0.1 0.4 < 0.1 0.5 < 0.1 0.5 < 0.1 Desha AR 0.2 0.2 0.1 0.1 0.2 0.2 0.4 < 0.1 0.4 < 0.1 0.6 < 0.1 0.6 < 0.1 0.4 < 0.1 0.4 < 0.1 Iberv/lle LA 0.5 0.5 0.6 0.6 Significant Effects W=Water C= Ceriodaphnia dubia S= Cyprinodon variagatua (•) Significant Difference Crustaceans - mortality. S=Sediment P= Pimephalas promotes 0= Daphni* pulex (_) No Significant Difference Fish - mortality, teratogenicity, or abnormal swimming behaviour. YPF - average number of young per female - applicable only to Ceriodaphnia dubia. Weight • applicable only to fish larval survival and growth test 15 ------- Station Number Waterbody Description Agency EPA Reach Latitude Longitude County State Sample Test Data of Collection Type Sptclts Test Percent Effected Control Percent Effected Test Control Significant Significant Test Control Significant Weight Weight Dtf In Effects YPF YPF DtflnYPF (mg) (ing) Weight Test Duration pH (Days) (S.U.J S-51 Mississippi River at Belle Chasse near West Bank Ferry Landing 16-Jul-91 W C 16-Jul-91 W P 0 0 0 10 814.8 15.9 O 0.533 0.493 0 7 7 7.7 7.7 S-52 Mississippi Rhw near East Bank Ferry Utntfng across Irorn Bell Chasse 13-Aug-91 W C 13-Aug-91 W P 10-Sep-91 W C 10-Sep-91 W P 150ct-91 W C 15-Oct-91 W P 19-Nov-91 W C 19-NW-91 W P 11-Dac-91 W C 11-D«c-91 W P 07-Jan-92 W C 07-Jan-92 W P 12-Feb-92 W C 12-Fab-92 W P 11-Mar-92 W C 11-Mar-92 W P 08-Apr-92 W C OS-Apr-92 W P 13-May-92 W C 13-May-92 W P 10 10 0 0 0 0 20 13 0 17 10 13 0 7 0 3 0 0 10 10 10 17 0 7 0 3 10 0 0 17 0 7 0 7 0 3 0 0 10 10 O 14.8 16-2 Q Q 18.7 16.4 Q Q 16.3 16.1 Q Q 9 13.7 Q Q 15.3 19 Q Q 9-2 12.2 Q Q 17.5 18.3 Q Q 17.8 16.9 Q Q 19.2 20.3 fj Q 20.3 19.1 o 0 0 o (§) o ® o o o o 0.372 0.343 0.814 0.844 0.522 0.448 0.709 0.64 0.602 0.602 0.302 0.273 0.361 0.38 0.407 0.469 0.412 0.448 0.321 0.305 o o o 0 o 0 o 0 o o 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 8.1 8.1 8.4 8.4 7.6 7.6 7.9 7.9 7.6 7.6 7.9 7.9 7.7 7.7 7.3 7.3 7.3 7.3 7.1 7.1 S-53 Mississippi Rrver near West Bank Ferry Landing at Placfjemlne 11-Jun-91 W C 11-Jun-91 W P 0 0 0 0 Q 17.1 16.8 0 0.678 0.695 o 7 7 7.7 7.7 S-54 Mississippi River near East Bank Ferry Landi ng across from Ptaquomtne 14-May-91 W C 14-May-91 W P 16-Jul-91 W C 16-Jul-91 W P Sample Types Test Species 0 0 20 7 0 10 0 10 Q 15.5 16.2 Q Q 14.4 15.9 O o o 0.55 0.537 0.528 0.493 o o 7 7 7 7 7.5 7.5 7.1 7.1 Total Total Unionized Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-N (man.) (mg/t) (umhosfcm) (mg/L) (rngfl.) (mg/|) LDEQ 122 122 LDEQ 139 139 130 130 130 130 105 105 88 88 99 99 119 119 101 101 84 84 104 104 LDEQ 96 96 LDEQ 84 64 130 130 167 389 167 389 08090301040 188 393 188 393 180 461 180 461 393 478 393 478 138 453 138 453 119 346 119 346 127 283 127 283 103 367 103 367 138 345 138 345 131 273 131 273 163 266 163 266 08070100007 120 292 120 292 08070100007 104 274 104 274 175 385 175 385 29-5ri1" 89'58'SO- < 0.1 < 0.1 29*5ff54- 89'5ff41" < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 so-iT-or gnur < 0.1 < 0.1 30M7W 9rir45- < 0.1 < 0.1 < 0.1 < 0.1 Flaquemlnes LA 0.2 0.2 ttwrvttte LA 0.4 0.4 0.6 0.6 0.4 0.4 0.5 < 0.1 0.5 < 0.1 0.4 0.4 0.6 < 0.1 0.6 < 0.1 0.5 < 0.1 0.5 < 0.1 0.5 < 0.1 0.5 < 0.1 0.5 < 0.1 0.5 < 0.1 2.4 < 0.1 2.4 < 0.1 Hienaie LA 0.7 0.7 Jbarvttte LA 0.5 0.5 0.4 0.4 Significant Effects W-Water C= Cenbdap/im. dob* S= CyfrinoOon varwoaftu MM Significant Difference Crustaceans - mortality. S=Sedlment P= Pmaphmhi pmmafu D= Depnnfe pufex Q No Significant Difference Fish - mortality, tentogenicity, or abnormal swimming behaviour. YPF-average number of young per female-applicable only to Cenbdap/infe duUa. Weight - applicable only to fish larval survival and growth test 16 ------- Station Number Watertody Description Agency EPA Reach Latitude Longitude County State Date of Collection Continued... 13-Aug-91 13-Aug-91 10-Sep-91 10-Sep-91 15-Oct-91 15-0ct-91 19-Nov-91 19-Nov-91 11-Dec-91 11-Deo-91 07-Jan-92 07-Jan-92 12-Feb-92 12-Feb-92 11-Mar-92 11-Mar-92 08-Apr-92 OS-Apr-92 13-May-92 13-May-92 UPR21 1.001525 24-Sep-90 24-Sep-90 UPR21 1.001535 24-Sep-90 24-Sep-90 UPR21 1.001536 24-Sep-90 24-Sep-90 UPR211.001537 24-Sep-90 24-Sep-90 Sample Test Type Species Test Control Percent Percent Effected Effected Test Control Significant Significant Test Control Significant Weight Weight Dlfln Effects YPF YPF DrHnYPF (mg) (mg) Weight Test Duration (Days) pH (S.U.) Alkalinity Hardness Conductivity (mg/L) (mg/L) (umhosfcm) Total Total Chlorine Ammonla-N (mgfl.) (mg/L) Unionized Ammonla-N (mgfl) Mississippi River near East Bank Ferry Landing across from Plaquemlne _, . W C W P W C W P W C W P W C W P W C W P W C W P W C W P W C W P W C W P W C W P 0 7 0 10 0 0 0 3 0 7 10 17 0 3 0 7 0 13 0 17 10 17 0 7 0 3 10 0 0 17 0 7 0 7 0 3 0 0 10 10 Q 15.6 16.2 O Q 0.329 0.343 Q 18-3 16.4 O Q 0.908 0.844 Q 15.7 16.1 O Q 0.514 0.448 Q 11.9 13.7 O Q 0.669 0.64 Q 16 19 O Q °-593 0.602 Q 1° 12.2 (•) Q 0-27 0.273 Q 17.4 18.3 O Q 0.381 0.38 Q 19 16.9 O Q 0-408 0.469 Q 22.6 20.3 O Q 0.337 0.448 Q 21.6 19.1 O O 0-321 0.305 O O O O O O O O (§) O 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 8.3 8.3 8.3 8.3 7.9 7.9 8.0 8.0 7.5 7.5 7.7 7.7 7.9 7.9 7.6 7.6 6.7 6.7 7.2 7.2 Galllnas River below WWTP W C W P 0 7 10 3 O 18.5 17.2 O 7 7 8.0 8.0 Ga///nas River above WWTP W C W P 0 10 10 3 816.3 17.2 O 7 7 8.0 8.0 Pacos Arroyo above Galllnas River W C W P 0 0 10 3 O 16 17.2 O O 7 7 7.9 7.9 Spring Arroyo at Airport Road W C W P 100 30 10 3 Sample Types Test Species Significant Effects 7 7 7.9 7.9 140 179 140 179 130 164 130 164 139 172 139 172 88 150 88 150 90 107 90 107 101 134 101 134 113 142 113 142 101 142 101 142 82 126 82 126 104 167 104 167 NMED 231 452 231 452 NMED 226 476 226 476 NMED 186 582 186 582 NMED 211 280 211 280 388 388 376 376 431 431 426 426 310 310 276 276. 353 353 350 350 264 264 297 297 785 785 851 851 963 963 449 449 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 « 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 « 0.1 < 0.1 « 0.1 « 0.1 < 0.1 < 0.1 < 0.1 0.2 0.2 0.5 0.5 0.5 0.5 0.8 0.8 0.2 0.2 0.4 0.4 0.8 0.8 0.1 0.1 0.4 0.4 0.4 0.4 0.5 0.5 0.5 0.5 0.5 0.5 1.2 1.2 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 NM NM NM NM W=Water C= Cenxlaphnm dubm S= Cyprinodon twfegalus (•) Significant Difference Crustaceans - mortality. S=Sediment P= Pimephalos promotes D= Daphni* puhx Q No Significant Difference Fish • mortality, teratogenicity, or abnormal swimming behaviour. YPF - average number of young per female - applicable only to Coriod»phnit dubm. Weight - applicable only to fish larval survival and growth last 17 ------- Station Number Waterbody Description Agency EPA Reach Latitude Longitude County State Tort Control Test Control Significant Tast Total Total Unionized Sample Tart Parcant Paicant Slgnlflcant Tact Control SlgnMcant Walght Walght OH In Duration pH Alkalinity Hardnass Conductivity Chlorlna Ammonla-N Ammonla-N DataofCollactlon Typa Spaclaa Effactad EfteM Effects YPf YPF DHInYPF (mg) (mg) WalgM (Dayt) (S.U.) (mgfl.) (mflfl.) (umhoalcin) (rng/L) (mgft.) (mgfl) UPR211.001538 24-Sep-90 24-Sep-90 UPR21 1.001539 24-Sep-SO 24-Sep-90 URO119.023505 25-Jul-89 25-Jul-89 URO1 19.023510 25-Jul-89 25-JUI-89 URG119.023515 25-Jul-89 25-Jul-89 URG1 19.023535 25-Jul-a9 25-Jul-89 OB002 20-Mar-90 20-Mar-90 17-Sap-90 11-Dec-90 11-Oec-90 20B 23-Jul-90 23-Jul-90 30-0c<-90 Pecos Arroyo at Harris Lake W C 20 W P 3 Galllnas River at Grand W C 60 W P 13 10 3 10 3 O 15.4 17.2 (5) 0 17.2 O (§) 7 7 7 7 8.0 8.0 8.3 8.3 Rkt Pueblo de Taoa at the Rio Grande W C 0 W P 10 0 3 O 18 19.2 O 7 7 8.6 8.6 Rto Puebto de Taoa. Upper End of the Lower Canyon WC 0 W P 7 Rto Puebto de Taoa Below the Taot W C 0 W P 13 0 3 O 20.7 19.2 O 7 7 8.4 8.4 i WWTF and Above USGS Guage 0 3 O 15.8 19.2 O 7 7 8.3 8.3 Rto Pueblo de Taoa above the Taoa Waatewater Treatment Facility W C 0 W P 10 0 3 8 '" ~ 0 7 7 8.2 8.2 Bird Creek at Seth Street North Bridge In Tulsa W C 0 W P 0 W C 40 W C 0 W P 7 Tar Creek at 22nd Avenue hi Miami W C 100 W P 100 W C 100 Sample Tvoat Taat Specie* W=Watar O Carfodaonnia dubm S= Cvorinodan vm 0 0 10 10 7 O 18 17.7 O 7.2 14.1 Q 20.4 19.4 0 7 7 7 7 7 7.4 7.4 7.3 7.0 7.0 NMED 148 472 148 472 NMED 246 384 246 384 843 843 562 562 NMED 13020101013 194 253 194 253 450 450 NMED 13020101013 217 273 217 273 470 470 NMED 13020101013 231 286 231 286 492 492 NMED 13020101013 233 292 233 292 499 499 WCOG 11070107001 74 98 74 98 84 120 83 152 83 152 189 189 383 534 534 OWRB 11070206014 0 3 0 ieoatus Siflnificant Effects Ciuttaceant - mortalitv. 1 1 1 4.3 4.3 5.9 500 2000 500 2000 12 2000 YPF - averaoa numb 1980 1980 2050 Brof vouna < 0.1 < 0.1 < 0.1 < 0.1 0.7 0.7 0.5 0.5 36-20-20- 105M3M5- Taos < 0.1 < 0.1 0.6 0.6 NM NM NM 36*21'ir 105*4?ir Taoa NM < 0.1 < 0.1 0.7 0.7 36*22438- 105'40'Or Taoa « 0.1 < 0.1 1.6 1.6 36'22-49- 105-39-44" Taoa < 0.1 < < 0.1 < 36M4W 95M9-50- < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 X'SSSSr 94'52'OS" < 0.1 < 0.1 < 0.1 ner femala - aoolieabl* on 0.1 0.1 Tulsa 0.5 0.5 0.2 1.7 1.7 Oltowa 1.2 1.2 0.5 h/to Cmriodfnhn NM nMv OK OK fcduMe. (_) No Significant Difference R*h - mortality, toratoganicity, or abnormal cwimming behaviour. Weight - appllcabla only to Man larval tutvival and growth teat 18 ------- Station Number Waterbody Description Sample Test Date of Collection Typt Species Ttst Percent Effected Continued.- Tar Creek at 22nd Avenue In Miami Control Test Control Significant Test Percent significant Test Control Significant Weight Weight Wf In Duration pH Effects YPF YPF DtflnYPF (mg) (mg) Weight (Days) (S.U.) Agency EPA Reach Latitude Longitude County State Total Total Unionized Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-N (rngfl.) (mgfl.) (umhos/cm) (mg/L) (mfl/|j (mg/l) 30-Jan-91 30-Jan-91 7 23-Jul-90 23-Jul-90 30-Od-90 30-Jan-91 30-Jan-91 W W C P 100 100 0 0 1 1 6.1 6.1 Tar Creek at Oklahoma/Kansas State Line North of Miami W W W W W C P C C P 100 100 100 100 100 0 0 0 0 0 © © © © ® 1 1 1 1 1 7.3 7.3 7.4 6.0 6.0 51 51 OWRB 115 115 118 49 49 640 640 957 957 11070206014 906 906 960 280 280 1259 1259 1496 443 443 < 0.1 < 0.1 36'59i54" 94'5V05" < 0.1 < < 0.1 < < 0.1 < 0.1 < 0.1 15 15 Ottawa 0.1 0.1 0.1 0.2 0.2 OK OCC1 Lake Creek at SH152 Bridge above Fort Cobb Reservoir OCC 11130302037 35'1724" 98'31'44" Cadbb OK 14-May-90 14-Aug-90 14-Aug-90 10-Sep-90 24-Sep-90 24-Sep-90 21-0ct-90 21-Oct-90 OCC2 09-Apr-90 09-Apr-90 15-Apr-90 14-Aug-90 14-Aug-90 10-Sep-90 16-Oct-90 16-Od-90 21-Oct-90 21-Oct-90 24-Feb-91 24-Feb-91 28-Feb-91 28-Feb-91 28-Feb-91 W W W S W W S S C C P D C P D P 0 0 13 0 0 7 0 3 Galnes Creek near Lake Eufala, W W S W W S W W S S W W W W S C P D C P D C P D P C P C P C 0 10 0 0 3 0 0 3 0 7 0 7 60 7 40 0 0 3 0 10 3 0 3 Q 18.5 15.2 Q 18.7 17.2 o Q Q 18.1 17.2 o Q o 8 O 7 7 7 2 7 0 2 7 8.3 7.9 7.9 8.0 8.2 8.2 7.5 7.5 abouf 1.25 Miles Northwest of Gotten 0 7 0 0 3 0 0 10 0 3 0 3 10 0 10 O 17.6 16 Q Q Q 16.8 175 o Q Q 19-6 18 Q Q Q Q 15.7 17.7 Q © 9 18.1 Q O 9-4 18.1 Sample Types Test Species O O o o © © Significant Effects 7 7 2 7 7 2 7 7 2 7 7 7 7 7 7 6.4 6.4 7.9 6.6 6.6 6.9 6.4 6.4 6.6 6.6 5.3 5.3 6.1 6.1 6.5 275 233 233 145 251 251 137 137 OCC 7 7 40 10 10 108 2 2 180 180 1 1 1 1 172 290 269 269 124 252 252 128 128 600 485 485 338 339 339 308 308 11090204050 32 32 57 24 24 100 28 28 144 144 56 56 60 60 225 80 80 257 48 48 284 82 82 356 356 201 201 153 153 500 < 0.1 < < 0.1 « 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 34'54'OS- 95*2922" < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 « 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 « 0.1 < 0.1 < 0.1 < 0.1 0.1 0.4 0.4 0.7 0.7 0.7 0.7 0.7 Latlmer OK 0.5 0.5 0.4 0.4 2.4 0.4 0.4 1.9 1.9 9.6 9.6 2.2 2.2 2.6 W=Watof C=Coriodaphniadubia S= Cyprinodon variogatui © Significant Difference Crustaceans - mortality. SsSediment P= Pimephfha prometai D* Daphnia pu/sx (_) No Significant Difference Fish - mortality, teratogenicity, or abnormal swimming behaviour. YPF - average number of young per female - applicable only to Ceriodaphnia dubia. Weight - applicable only to fish larval survival and growth test 19 ------- Station Number Waterttody Description Agency EPA Reach Latitude Longitude County State Date of Collection Continued... 28-Feb-91 16-Jul-91 16-Jut-91 2cXlul-91 28^>ul-91 OCC3 2ftOul-91 28-JuU91 OCC4 28-Juk91 28-Jul-91 Test Control Test Control Significant Sample Test Percent Percent Significant Test Control Significant Weight Weight Dlf In Type Species Effected Effected Effects YPF YPF DiflnYPF (mg) (ing) Weight Galnes Creek near Lake Eufala, about 125 Miles Northwest of Gowen S P W C W P S C S P 10 0 17 50 20 0 0 10 0 10 Q Q 14.6 15.9 o ® 8.8 14.9 o o 0.553 0.493 Q (•) Test Duration pH (Days) (S.U.) 7 7 7 7 7 6.5 6.2 6.2 6.4 6.4 Lake Eufala Northwest ofHwy 31 Bridge S C S P 0 7 0 10 815.4 14.9 O 7 7 6.2 6.2 Lake Eufala atAdamaon Road f 0.5 Mites EastofMcAlester S C S P 10 7 0 10 817.6 14.9 o 7 7 6.6 6.6 Total Total Unionized Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonia^ (mgfl.) (mgfl.) (umhos/cm) (mgfl.) (mg/L) (mgfl) 172 225 27 75 27 75 109 104 109 104 500 < 0.1 230 < 0.1 230 < 0.1 324 < 0.1 324 < 0.1 OCC 22060050010 34*58'39* 95'3T43" 90 92 90 92 279 < 0.1 279 < 0.1 OCC 22060050010 34*5578' 95*34'16" 210 175 210 175 2.6 0.6 0.6 1.1 1.1 Ptttsrjurg 1.7 1.7 Ptttsburg OK OK 450 < 0.1 12.5 450 < 0.1 12.5 OCC5 Pitt Creek 2 Miles South of US 270 3.5 Miles East ofHartshome OCC 22060040040 34*5ri4' LaUmer OK 27-Feb-91 27-Fab-91 28-Fab-91 28-Feb-91 16-Jul-91 16-JUI-91 26-Jul-91 26-Jul-91 W W S S W W S S C P C P C P C P 100 100 100 100 100 100 100 100 o ( o ( 0 ( o ( 0 ( o ( 0 ( 0 ( -v ) ' . ) ) . . . * j : 3.1 3.1 3.7 3.7 2.7 2.7 3.8 ! 3.8 0 0 0 0 0 0 0 0 0 0 231 231 650 650 238 238 1977 1977 684 684 1980 1980 681 681 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 16.8 16.8 2.6 2.6 15.9 15.9 4.8 4.8 OCC6 Deep Fork One Mile East oflH 35, One Mile South ofH4SE ofEdmond OCC 52071002001 35*34'4r 97*2539* Oklahoma OK 07-May-91 W C 07-May-91 S P uX-Jun-91 S C 04-Jun-91 S P OS-Nov-91 W C 05-Nov-91 W P 13-Nov-91 S C 13-N0V-91 S P 0 3 10 7 10 3 0 7 20 10 0 3 0 3 0 0 Q 18-3 15.6 Q Q 14.9 15.5 (~) Q 13 12 Q Q 11-3 10.5 o O o o 0 7 7 7 7 7 7 7 7 7.9 7.9 7.6 7.6 8.4 8.4 7.5 7.5 OCC7 Spring Creek West of OS and South of US77 East of Edmond 07-May-91 W C Sample Types Test Species W=Water C= Carfedapnnile dub* 100 8s Cyorinoda 10 iimtr ® V^5' Stflnincent Difference (1 MA CisinHlf.n+ HiM«w« Significant Effect* Crustacean* - mortality. 1 7.8 236 236 224 224 286 286 269 269 260 260 180 180 315 315 221 221 419 419 603 603 783 783 434 434 OCC 52071002003 250 YPF- 320 594 < 0.1 0.4 < 0.1 0.4 < 0.1 0.9 < 0.1 0.9 < 0.1 0.7 < 0.1 0.7 < 0.1 1.3 < 0.1 1.3 35*2ff4r 97*25'30" < 0.1 0.5 average number of young per female - applicable only to Oklahoma OK Caribdap/mfa dub* 20 ------- Station Number Watertody Description Test Sample Test Percent Date of Collection Type Species Effected Continued.^ 04-Jun-91 04-Jun-91 OS-Nov-91 OS-Nov-91 13-Nov-91 13-Nov-91 ODEQ10 08-Apr-92 08-Apr-92 08-Apr-92 08-Apr-92 12-May-92 12-May-92 20-Aug-92 20-Aug-92 20-Aug-92 20-Aug-92 12-Oct-92 12-Oct-92 ODEQ12 08-Apr-92 08-Apr-92 08-Apr-92 08-Apr-92 20-Aug-92 20-Aug-92 20-Aug-92 20-Aug-92 12-Oc)-92 12-Oct-92 ODEQ2 15-Mar-90 18-Mar-90 Control Test Control Significant Test Percent Significant Test Control Significant Weight Weight Dlfln Duration pH Effected Effects YPF YPF Dlfln YPF (mg) (ing) Weight (Days) (S.U.) Spring Creek Westof I3S and South of US77 East of Edmond S C 0 0 Q 14.5 15.5 S P W C W P S C S P Acid Creek W C W P S C S P W C W P W C W P S C S P W C W P 13 60 3 10 3 0 13 0 7 10 100 0 3 10 3 0 10 3 Q 0 ® 7.8 12 3 Q 0 Q 12.7 1°-s o O 0 Q 22.4 20.3 10 Q 10 Q 3.3 13.7 7 Q 10 Q 16 19-1 0 Q 17 15.7 3 Q 0 Q 15.4 15.7 3 Q 0 Q 15.2 15.1 7 O O ® O o ® o 0 0 o 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7.5 7.5 8.3 8.3 7.8 7.8 8.3 8.3 6.9 6.9 8.2 8.2 7.8 7.8 7.9 7.9 8.1 8.1 Tributary of Chtckaskla River near Blackwell W C W P S C S P W C W P S C S P W C W P 50 3 30 10 100 3 100 100 100 100 0 ® 8.2 20.3 10 Q 10 Q 11.1 13.7 7 Q 0 ® 3 Q 0 ® 3 ® 0 ® 7 ® ® ® 7 7 7 7 4 7 2 7 1 7 7.4 7.4 7.8 7.8 6.8 6.8 7.6 7.6 6.8 6.8 Clmarron River North of Lone Mountain W S S D 100 0 Sample Types Test Species W=Water S= Sediment C= Coriodaphnia dubia P- Prmaonatos oromafe* S= Cyprinodon varfogafus ® Significant Difference D= Deo/wife Dufax O No Significant Difference Significant Effects Crustaceans - mortality. Fish - mortality, teratogenicitv. or at 7 2 7.9 8.1 Agency EPA Reach Latitude Longitude County State Total Total Unionized Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-fl (mg/L) (mg/L) (umhos/cm) (man.) (mg/t) (mg/l) 212 172 212 172 256 299 256 299 214 221 214 221 ODEQ 142 228 142 228 168 145 168 145 362 371 362 371 122 166 122 166 182 199 182 199 136 167 136 167 ODEQ 300 665 300 665 212 233 212 233 78 77 78 77 158 146 158 146 62 142 62 142 ODEQ 0 1000 284 196 546 546 738 738 475 475 930 930 504 504 1030 1030 456 456 425 425 615 615 1515 1515 472 472 456 456 447 447 463 463 1673 950 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 36'4ff52- 97'03'54" « 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 « 0.1 < 0.1 < 0.1 « 0.1 36'48'33- 97M7-1 1" < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 36-2T22- 98Mr57" < 0.1 < 0.1 < 1.2 1.2 1.3 1.3 0.6 0.6 0.5 0.5 1.9 1.9 0.3 0.3 0.4 0.4 1.8 1.8 0.4 0.4 1.3 1.3 3.2 3.2 0.4 0.4 0.6 0.6 0.2 0.2 0.4 0.1 YPF - average number of young per female - applicable only to 'normal swimming behaviour. Weiaht - aoolicable only to fish larval survival and arowth test ••*•• • Kay OK t Kay OK Major OK Ceriodaphnia dubia.. 21 ------- Station Number Waterbody Description Agency EPA Reach Latitude Longitude County State Sample Test Date of Collection Type Species Continued... 18-Mar-90 09-May-90 18-Jul-90 24-Jul-90 24-Jul-90 10-Od-90 07-Apr-91 07-Apr-91 IS-May-91 26-AU0-91 12-Nov-91 30-Mar-92 30-Mar-92 05-May-92 12-Aug-92 12-Aufl-92 08-Oct-92 OOEQ3 05-Mar-90 05-Mar-90 11-Mar-90 11-Mar-90 07-May-90 07-May-90 11-Jul-90 11-Jul-90 25-Jul-90 25-Jul-90 03-Oct-90 24-Mar-91 24-Mar-91 24-Mar-91 24-Mar-91 07-May-91 07-May-91 Sample Types Test Effected Control Test Control Significant Test Percent significant Test Control Significant Weight Weight Wf In Duration pH Effected Effects YPF YPF DtflnYPF (mg) (mg) Weight (Days) (S.U.) Alkalinity (mg/L) Hardness (man.) Conductivity (umhosfcm) Total Total Unionized Chlorine Ammonla-N Ammonla-N (mfln.) (mg/L) (mgfl) dmarron River North of Lone Mountain S W W S S W W S W S W W S W W S W P S S D P S S S S S S S S S S S S 10 100 100 0 3 100 100 10 100 10 100 100 0 100 100 13 100 3 Q 7 0 © 9 7 ® 8 0 Q 2 3 Q 7 3 ® 8 0 © 6 7 Q 8 0 © 6 3 Q 8 0 © 6 3 © 6 3 Q 9 3 © 5 0 © 7 3 O 7 3 ® 7 8.1 8.4 8.1 8.3 8.3 8.0 7.9 7.2 7.9 8.0 8.0 8.0 8.0 7.9 7.9 7.5 7.8 Coal Creek near Henryetta W W S S W W W W S S W W W S S W W Test Species c P 0 P c P c P 0 P c c P c P c P 100 100 100 37 100 20 100 100 100 100 100 100 100 100 100 100 100 0 ( 0 ( 0 ( 10 ( 0 ( 0 ( 0 ( 0 ( 0 ( 0 ( 0 ( 0 ( 0 ( 0 ( 0 ( 0 ( 0 ( ) 1 ) 2 ) 1 ) 7 ) 1 ) 5 ) 1 ) 5 ; 1 ) 5 > 1 ) 7 ) 1 ) 2 Significant Effect* 5.3 5.3 7.9 7.9 62 6.2 6.3 6.3 6.7 6.7 5.9 6.2 6.2 6.5 6.5 6.1 6.1 284 198 166 187 187 131 176 347 146 239 195 179 368 156 116 182 102 196 0 1097 244 244 1000 0 0 1000 3000 2000 1450 138 870 2000 950 10160 17100 1737 1737 21300 38200 29840 40700 32500 25700 37700 6200 27000 23600 25500 26500 OOEQ 3! 4 4 138 138 22 22 5 5 84 84 4 28 28 216 216 12 12 1000 1000 165 165 600 600 600 600 1503 1503 1000 1000 1000 370 370 1000 1000 1311 1311 840 840 1210 1210 1613 1613 2080 2080 1423 1006 1006 723 723 1205 1205 < 0.1 < < 0.1 < 0.1 < 0.1 « 0.1 < 0.1 < 0.1 < < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 J^TW 95'sror < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 0.1 1.0 0.7 0.5 0.5 0.1 1.0 0.5 1.0 72 1.2 0.7 1.9 02 1.9 22 024 OtanOgM OK 1.0 1.0 0.4 0.4 2.0 2.0 3.0 3.0 1.3 1.3 0.7 0.4 0.4 0.4 0.4 0.5 0.5 W=W«ter OCenboaphnfcduMa S= Cyprinodon ranogefus ® Significant Difference Cfuctaceans - mortality. S=Sediment P= PlrmpnaJn pmmelti D= Dap/into potot O N° Significant Difference R*h - mortality, temtogenicity, or abnormal swimming behaviour. YPF-average number of young per female-applicable only to Canbdapnnfc duMa. Weight - applicable only to fish larval survival and growth last 22 ------- Station Number Waterbody Description Test Sample Test Percent Date of Collection Type Species Effected Continued... 07-Aug-91 07-Aug-91 13-Aug-91 13-Aug-91 05-Nov-91 OS-Nov-91 ODEQ4 05-Mar-90 05-Mar-90 11-Mar-90 11-Mar-90 11-Jul-90 11-Jul-90 24-Jul-90 24-Jul-90 ODEQ6 09-Apr-91 09-Apr-91 14-Apr-91 14-Apr-91 28-May-91 28-May-91 14-Aug-91 20-Aug-91 20-Aug-91 ODEQ7 24-Mar-91 24-Mar-91 24-Mar-91 24-Mar-91 07-May-91 07-May-91 07-Aug-91 Control Test Control Significant Test Percent Significant Test Control Significant Weight Weight Dlf In Duration Effected Effects YPF YPF Dlf In YPF (mg) (mg) Weight (Days) pH (S.U.) Agency EPA Reach Latitude Longitude County State Total Total Unionized Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-N (mg/L) (mg/L) (umhosfcm) (mg/L) (mgfl.) (mgfl) Coal Creek near Henryetta W W S S W W C P C P C P 10 17 100 100 100 100 10 Q 17.4 17-2 O 10 Q 0 ® 0 (•) 0 (8) 0 (•) 7 7 3 3 1 2 7.5 7.5 6.2 6.2 5.1 5.1 Lake Francis at Upper End W W S S W W S S C P D P C P D P 0 7 0 3 10 3 0 0 0 Q 15.6 13.8 O 7 Q 0 Q 10 Q 0 Q 8-9 12 (§) 3 Q 0 Q 3 O 7 7 2 7 7 7 2 7 7.3 7.3 8.4 8.4 7.9 7.9 7.2 7.2 Bitter Creek W W S S W W S W W C P C P C P C C P 0 10 0 13 0 7 0 100 20 10 Q 16-3 15.8 O 7 Q 10 Q 19.6 15.1 O 3 Q 0 Q 13.8 13.6 O 3 Q 0 Q 16 14.4 O 0 ® 7 O 7 7 7 7 7 7 21 1 7 8.0 8.0 7.5 7.5 7.5 7.5 7.8 8.5 8.5 Tributary of San Bols Creek W W S S W W W C P C P C P C 100 100 100 100 100 100 0 Sample Types Test Species 0 ( ) 0 ( ) 0 ( ) 0 ( ) 0 ( ) 0 Q 10 O 16-2 17.2 O Significant Effects 1 7 1 7 1 2 7 6.6 6.6 6.6 6.6 6.2 6.2 6.4 116 116 92 92 21 21 ODEQ 82 82 120 120 100 100 127 127 146 146 175 175 1925 1925 107 107 184 184 114 114 124 124 532 532 514 514 1471 1471 162 162 574 574 181 181 379 379 ODEQ 196 196 244 244 198 198 189 206 206 1421 1421 317 317 832 832 348 2150 2150 4990 4990 997 997 2530 2530 1136 6490 6490 ODEQ 23 23 160 160 30 30 32 32 32 181 181 52 52 12 78 78 1062 1062 89 89 156 0.1 0.1 « 0.1 < 0.1 < 0.1 < 0.1 36'0275" 94'33'33" < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < < 0.1 < < 0.1 < 0.1 34'35'38- aa-23fM' * 0.1 < 0.1 < 0.1 < 0.1 < 0.1 « 0.1 < 0.1 < 0.1 < 0.1 37'05130- 95*21'05" < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 0.1 0.6 0.6 2.2 2.2 1.4 1.4 Adalr OK 0.4 0.4 2.6 2.6 0.1 0.1 0.2 0.2 Jackson OK 0.4 0.4 3.6 3.6 1.2 1.2 1.7 0.6 0.6 Plttsburg OK 0.2 0.2 0.2 0.2 0.5 0.5 0.1 W=Water O Ctriodaphnia dubia S= Cyprinodon varitgalua (*) Significant Difference Crustaceans - mortality. S=Sediment P= Pimophalaa promotes D= Daphnia pufetx (_) No Significant Difference Fish - mortality, teratogenicity, or abnormal swimming behaviour. YPF - average number of young per female - applicable only to Cariodaphnia dubia. Weight - applicable only to fish larval survival and growth test 23 ------- Station Number Waterbody Description Date of Collection Continued... 07-Aug-91 13-Aug-91 05-Nov-91 OS-Nov-91 ODEQ9 Sample Test Type Species Test Percent Effected Tributary of San Bots Creek W P 20 S C 100 WC 100 W P 100 Control Percent Significant Effected Effects 10 Q 0 ® Test Control Significant Test Test Control Significant Weight Weight Dtfln Duration YPF YPF DrfinYPF (mg) (mg) Weight (Days) 7 1 1 2 pH (S.U.) 6.4 6.5 6.0 6.0 Tributary of Eliza Creek Agency EPA Reach Latitude Longitude County State Alkalinity Hardness (mg/L) (mg/L) 32 143 21 21 ODEQ 12 104 38 38 Conductivity (umhosfcm) 156 367 105 105 Total Chlorine (mg/L) 0.1 < 0.1 < 0.1 < 0.1 Total Ammonla-M 0.1 4.3 1.3 1.3 V&AVtT QCtCtfOC" OO nj 0 1 09 99 O9 Unionized Ammonla-N (mg/1) OK 24-Mar-92 24-Mar-92 24-Mar-92 24-Mar-92 20-May-92 2(Hvtay-92 25-Aug-92 25-Aug-92 08-Sep-92 08-Sep-92 21-Oct-92 21-Oct-92 W W S S W W W W S S W W C P C P C P C P C P C P 100 100 100 13 100 15 100 7 100 17 100 16 0 ® 7 (•) 0 ® 7 Q 0 ® 7 Q 0 ® 3 Q 0 ® 3 Q 0 ® 3 O 1 7 1 7 3 7 1 7 1 7 1 7 8.0 8.0 7.2 7.2 7.4 7.4 7.4 7.4 7.4 7.4 7.3 7.3 306 306 238 238 310 310 422 422 170 170 162 162 443 443 213 213 404 404 443 443 240 240 207 207 1170 1170 630 630 960 960 903 903 642 642 614 614 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 0.9 0.9 0.4 0.4 0.4 0.4 0.2 0.2 0.8 0.8 0.4 0.4 TA615 Mlngo Creek at Apache Street Bridge In Tutea INCOG 36M1-31- 95'51'39" Tutea OK 20-Mar-90 20-Mar-90 17-Sep-90 11-Dec-90 11-D0C-90 H-Dec-90 11-Dec-90 0214.0160 04-Nov-92 04-Nov-92 08-Jun-93 08-Jun-93 0304.0100 25-Jan-93 Sample Types U/-U/*htr W C W P W C W C W P S C S P 10 7 0 0 0 0 7 o Q 10 Q 10 Q 10 Q o Q o Q 7 O 16.4 17.7 17.3 14.1 21.6 19.4 16.2 17.6 O 8 0 7 7 7 7 7 7 7 7.9 7.9 7.4 7.0 7.0 7.4 7.4 Wichita River at End ofEasttand Lane W C W P W C W P 0 0 20 3 10 Q o Q 0 Q 3 O 8.9 16.3 19.1 18 ® O 7 7 7 7 7.9 7.9 7.3 7.3 Days Creek af Statellne Road South of Texattana W C Test Species 0 o O 21.8 19.2 o Significant Effects 7 6.8 249 249 74 147 147 149 149 294 420 294 420 144 365 248 608 248 608 200 382 200 382 77VRCC 11130206001 204 204 188 188 1097 3710 1097 3710 956 433 956 433 TlfVftCC 11140302003 28 41 144 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 ss-sffis" gs^e-so- < 0.1 < 0.1 0.2 0.2 33*22"15" 94*02'33" < 0.1 0.5 0.5 0.4 0.2 0.2 1.0 1.0 Wichita TX 0.5 0.5 1.2 1.2 Bowie TX 1.08 Fish - mortality, teratogenicity, or abnonnal swimming behaviour. Weight - applicable only to fish larval survival and growth test 24 ------- Station Number Waterbody Description Agency EPA Reach Latitude Longitude County State Sample Test Date of Collection Type Species Continued.. 25-Jan-93 25-Jan-93 25-Jan-93 06-Jul-93 06-Jul-93 06-Jul-93 06-Jul-93 0404.0020 03-Dec-91 03-Dec-91 . 11-Feb-92 11-Feb-92 21-Mar-92 21-Mar-92 04-Aug-92 04-Aug-92 12-Jan-93 12-Jan-93 12-Jan-93 12-Jan-93 28-Jun-93 28-Jun-93 28-Jun-93 25-Jun-93 0505.0300 09-Jan-90 09-Jan-90 27-Mar-90 27-Mar-90 09-Aug-90 16-Od-90 16-Oct-90 04-Feb-91 24-Apr-91 Sampl* Types Test Percent Effected Control Percent significant Effected Effects Test Control Significant Test Test Control Significant Weight Weight Difln Duration YPF YPF DlflnYPF (mg) (mg) Weight (Days) PH (S.U.) Total Alkalinity Hardness Conductivity Chlorine (mg/L) (mg/L) (umhosfcm) (mg/L) Total Unionized Amntonla-N Ammonla-N • (man.) (ma/i) Days Creek af Statellne Road South of Texarkana > W P S C S P W C W P S C S P 0 0 10 0 3 60 100 3 O o Q 3 Q 0 Q 0 Q 0 @ 10 (•) 21.4 19.2 20.9 20 1.2 19.1 O o ® 7 7 7 7 7 7 7 6.8 6.7 6.7 7.2 7.2 6.9 6.9 Big Cypress Crook at US 259 W C W P W C W P W C W P W C W P W C W P S C S P W C W P S C S P 0 10 0 7 0 10 0 3 10 7 10 0 0 7 0 0 0 Q 3 Q o Q 10 Q o Q 7 Q o Q 10 Q o Q 7 Q o Q 3 Q o Q o Q o Q 0 Q 17.4 16.7 17.1 17.5 21 16.8 18.2 17.4 18.9 20.1 19.7 20.7 22.1 20 22.3 20 o o o o 0 o o o 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 6.8 6.8 6.4 6.4 6.8 6.8 7.1 7.1 6.6 6.6 6.8 6.8 6.7 6.7 6.6 6.6 Sabtne River at AT&SF Railroad Southeast of Longvtew W C W P W C W P W C W C W P W C W C Test Species 0 3 0 10 0 0 7 0 30 0 Q 7 Q 10 Q 7 Q o Q o Q 10 Q 10 Q 10 Q 17.3 16.2 15.2 14.7 18.2 18.7 19.1 18 18 15.5 13.3 16.7 o 0 8 Significant Effects 7 7 7 7 7 7 7 7 7 7.1 7.1 6.8 6.8 7.3 6.7 6.7 7.1 6.7 28 171 171 34 34 200 200 TNRCC 21 21 23 23 22 22 26 26 19 19 216 216 25 25 137 137 TNRCC 55 55 37 37 36 33 33 64 35 41 144 165 375 165 375 91 480 91 480 151 519 151 519 11140305004 40 173 40 173 47 186 47 186 41 146 41 146 45 150 45 150 33 120 33 120 239 438 239 438 52 194 52 194 163 374 163 374 12010002021 53 256 53 256 41 159 41 159 53 263 34 191 34 191 40 179 48 74 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 32'54'18" 94' < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 « 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 1.08 2.4 2.4 0.3 0.3 19.2 19.2 43'25" Morris TX 0.5 0.5 0.4 0.4 0.4 0.4 0.4 0.4 0.36 0.36 4.08 4.08 0.5 0.5 1.9 1.9 32*24'56" 94*3ff12" Gregg TX « 0.1 < 0.1 « 0.1 < 0.1 < 0.1 « 0.1 < 0.1 < 0.1 < 0.1 « 0.1 < 0.1 0.4 0.4 < 0.1 0.4 0.4 0.5 0.5 W-Water C= Coriodaphni* dubi* S= Cyprlnodon iwiagwfu* (*) Significant Difference Crustaceans - mortality. S=Sediment P= Pimephulea promote D* Daphnit pubx (_) No Significant Difference Fish - mortality, teratogenicity, or abnormal swimming behaviour. YPF - average number of young per female • applicable only to Caribdaprin/e dubi*. Weight - applicable only to fish larval survival and growth test 25 ------- Station Number Waterbody Description Teat Control Ttst Control Significant Test Sample Test Percent Percent signMcant T«st Control Significant weight Wtlght Dlf In Duration pH Date of Collection Type Species Effected Effected Effects YPF YPF DWInYPF (mg) (mg) Weight (Daym) (S.U.) Continued... 24-Apr-91 07-Aug-91 07-Aug-91 0601.0800 13-Feb-90 13-Feb-90 14-May-90 27-NW-90 27-Nov-90 28-Fab-91 28-Feb-91 15-Apr-91 15-Apr-91 20-Aug-91 20-Aug-91 0611.0100 16-Jan-90 16-Jan-90 27-Mar-90 27-Mar-90 09-Aug-90 IO-Oct-90 11-Feb-91 30-Apr-91 30-Apr-91 28-Aug-91 0700.0200 05-Nov-91 05-Nov-91 06-Jan-92 06-Jan-92 23-Feb-93 23-Feb-93 Sample Types W=Water Agency EPA Reach Latitude Longitude County State Total Total Unionized Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-N (mgA.) (man.) (umhos/cm) (mg/L) (mg/L) (mg/1) Sablne River at AT ASF Railroad Southeast of Longvtew W P W C W P 10 3 Q 10 10 Q 7 10 O 14.7 17.2 O 7 7 7 6.7 7.6 7.6 Heches River Bridge on IH 10 Near Beaumont W C W P W C W C W P W C W P W C W P W C W P 10 10 Q 7 3 Q 0 0 Q 0 0 Q 0 0 Q 20 10 Q 3 0 Q o o Q 15 7 Q 10 20 Q 17 7 O 15.9 17.9 19.7 15.2 19.5 16.7 19.1 18.1 17.8 16.4 7.3 14.5 o 8 0 o (§) 7 7 7 7 7 7 7 7 7 7 7 7.0 7.0 6.6 6.2 6.2 6.1 6.1 6.3 6.3 6.3 6.3 Angelina River Bridge on US 59 North of LulUn W C W P W C W P W C W C W C W C W P W C 0 0 Q 7 3 Q 0 10 Q 3 7 Q 0 0 Q 0 0 Q 0 0 Q 0 10 Q 10 3 Q 0 0 O 17.5 18.4 16.5 14.7 16.9 18.7 18.8 16.5 16.5 15.1 15.3 15.2 16.1 14.4 o o o o Q o o 7 7 7 7 7 7 7 7 7 7 6.8 6.8 7.0 7.0 7.1 7.0 5.9 6.7 6.7 6.9 Alligator Bayou at SH 214 In Port Arthur W C W P W C W P W C W P Test Species C= Carfodaohnfe dublm 100 0 ® 100 o ® 100 0 ® 100 3 W o o Q 10 7 O S= Cvorinodon variboafus (•) Sk 21.2 19.9 mificant Difference o Significant Effects Crustaceans - mortality. 1 6 2 6 7 7 7.2 7.2 6.9 6.9 6.7 6.7 35 76 76 7WRCC 77 77 12 23 23 11 11 16 16 21 21 7NRCC 7 7 23 23 24 23 11 24 24 21 TNRCC 84 84 84 84 86 86 YPF -aver* 48 46 46 74 445 445 12020003003 119 119 40 140 140 20 20 24 24 92 92 362 362 106 1043 1043 85 85 106 106 108 108 12020005031 29 29 49 49 28 32 44 36 36 16 114 114 165 165 98 134 151 78 78 111 12040201010 143 143 79 79 121 121 Benumb 1680 1680 1135 1135 840 840 •rofvouna < 0.1 < 0.1 < 0.1 ao'os-sg- 94*0574- < 0.1 < 0.1 « 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 3-C2T2r 94M3-33" < 0.1 < < 0.1 < < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < < 0.1 < 0.1 < 0.1 29452137" 93'58'40" 0.5 0.3 0.3 Orange 7X 0.7 0.7 1.1 0.7 0.7 0.2 0.2 0.7 0.7 0.5 0.5 Nacogdochet TX 0.1 0.1 0.7 0.7 0.6 0.5 0.5 0.2 0.2 0.4 Jefferson TX < 0.1 10.0 < 0.1 10.0 < 0.1 < 0.1 < 0.1 < 0.1 oar female - anolicable onl 1.2 1.2 1.9 1.9 tvto Cfriodfohnif dubif No Significant Difference Fish - mortality, teratogenicity, or abnormal swimming behaviour. Weight - applicable only to (Mi larval survival and growth test 26 ------- Station Number Waterbody Description Agency EPA Reach Latitude Longitude County State Test Control Sample Test percent Percent Significant Test Date of Collection Type Species Effected Effected Effects YPF Continued... 23-Feb-93 23-Feb-93 19-Jul-93 19-Jul-93 19-Jul-93 19-Jul-93 03-Jan-94 03-Jan-94 03-Jan-94 03-Jan-94 0877.777? 13-Sep-90 0804.0400 13-Sep-90 0805.0050 27-Mar-90 27-Mar-90 31-Jul-90 31-Jul-90 10-Oct-90 22-Jan-91 09-Apr-91 09-Apr-91 0805.0100 13-Sep-90 0805.0300 13-Sep-90 01-Oct-91 01-Oct-91 21-Apr-92 21-Apr-92 Alligator Bayou at SH 214 In Port Arthur S C 00 S P 73 WC 00 W P 73 S C 100 10 S P 100 3 W C 00 W P 10 3 S C 100 0 S P 100 3 Clear Fork Trinity River below Lake Benbrook WC 0 10 Trinity River at US 79 Northeast of Oakwood W C 0 10 Trinity River at FM 85 East ofEnnls W C 0 10 W P 77 W C 0 10 W P 03 W C 00 W C 00 W C 10 10 W P 77 Trinity River at SH34 Southwest of Rosser W C 0 10 Q 21'6 Q Q 12.2 Q (•) @ Q 19.2 Q (•) (•) O i6.s O 16-i O 16-3 Q Q 20.2 Q Q 18-6 Q 18.9 O 14-1 o O 16.6 Test Control Significant Test Control Significant Weight Weight Dtfln Duration pH YPF DrflnYPF (mg) (mg) Weight (Days) (S.U.) 18.7 20.2 17.3 16.1 16.1 14.7 19.5 16.5 16.2 15.8 16.1 O 7 7 (§) 7 7 2 5 O 7 7 3 4 O 7 O 7 O 7 7 O 7 7 §7 7 7 7 0 7 7.4 7.4 8.2 8.2 7.4 7.4 6.8 6.8 6.9 6.9 7.4 7.3 7.8 7.8 7.7 7.7 7.6 6.8 7.6 7.6 7.6 Trinity River at South Loop SH 12 below Dallas W C 10 10 W C 10 0 W P 37 W C 00 W P 10 7 Q 18-2 Q 17.4 o Q 18.9 O 16.1 13.6 16.8 Sample Types Test Species W=Water S=Sediment C= Ceriodaphnia dubia S= Cyprinodon vanooafu* P= Pimaphabt pmmolaa r> Daphnia puhx ® Significant Difference O No Significant Difference 8 i 7 O 7 7 Significant Effects Crustaceans • mortality. 7.4 7.6 7.6 7.5 7.5 Total Total Unionized Alkalinity Hardness Conductivity Chlorine Ammonla-N Amnupla-N (mg/L) (mg/L) (umhos/em) (mg/L) (mgfl.) (ma/|) 217 217 110 110 187 187 32 32 TNRCC 142 TNRCC 86 TNRCC 127 127 119 119 88 131 124 124 TNRCC 108 TNRCC 105 118 118 122 122 133 133 161 161 140 140 104 104 721 721 1285 1285 808 808 1270 1270 927 927 12030102017 144 269 < < < < < < 0.1 9.6 0.1 9.6 0.1 0.4 0.1 0.4 0.1 15.6 0.1 15.6 0.2 0.8 0.2 0.8 32*39140- 97'2ffS2" < 12030201010 31'38'5- 144 435 12030105012 150 150 126 126 152 168 205 205 341 341 236 236 536 367 697 697 < 0.1 2.5 r 95*472r 0.1 1.0 32'18159- 96*21'33" < « < < « < 0.1 2.6 0.1 2.6 0.1 0.1 0.1 0.1 0.1 0.5 0.1 0.2 0.1 0.5 0.1 0.5 32'25-36- 95'2T45" 132 413 12030105016 140 164 164 180 180 456 640 640 366 366 < 0.1 1.0 32M2'27- 96'44'06" < < < < < 0.1 0.7 0.1 0.7 0.1 0.7 0.1 0.2 0.1 0.2 YPF - average number of young per female - applicable only to Fish - mortality, teratogenicity, or abnormal swimming behaviour. . Tarrant TX Anderson TX Navarro TX Kaufman TX Dallas TX Ceriodaphnia dubia. Weight - applicable only to fish larval survival and growth test 27 ------- Station Numb*- Watorkody Description Agency EPA Reach Latitude Longitude County State Sample Test Date of Collection Type Specie* Continued^. 29-JUI-92 01-Sep-92 01-Sep-92 0805.0400 13-Sep-90 0806.0005 13-Sep-ao 0841.0500 29-Sep-92 29-Sep-92 29-Sap-92 29-Sep-92 11-Aug-93 11-Aug-93 H-Aug-93 11-Aug-93 06-Oec-93 06-Dec-93 06-Dec-93 06-Dec-93 0902.0100 12-Feb-90 12-Feb-90 11-Mar-90 11-Mar-90 13-Nov-90 28-Feb-91 28-Feb-91 06-May-91 06-May-91 15-Aug-91 15-Aug-91 Sample Type* W'Water S= Sediment Te*t Pcrcwtt Effected Control Percent Significant Effected Effect* Test Control Significant Test Test Control Significant Weight Weight Drfln Duration YPF YPF DtflnYPF (mg) (mg) Weight (Days) PH (S.U.) Total Total Unionized Alkalinity Hardnen Conductivity Chlorine Ammonla-N Ammonla-N (mgfl.) (mgfl.) (umhotfcm) (mgfl.) (mOfl.) (mgfl) Trinity River at South Loop SH 12 below Dallas W C W C W P 10 10 7 10 Q 0 O 3 O 16.6 11.2 18.5 15.3 0 (f) 7 7 7 7.5 7.1 7.1 Trinity River at Commerce Street In Dallas W C 0 10 O 17.1 16.1 0 7 7.7 West Fork Trinity River at Beach Street In Fort Worth W C 0 10 O 18.2 16.1 0 7 8.4 Lower West Fork Trinity River at BeHHne Road in Grand Prairie W C W P S C S P W C W P S C S P W C W P S C S P 0 3 0 0 0 3 0 0 0 0 0 3 o Q o Q o Q o Q o Q 0 Q o Q 3 Q o Q 3 Q o Q 3 O 26.4 16.8 21.1 18.4 20.3 15.8 26.8 26.8 17.8 18.2 16.8 18.2 o ® 0 o 0 o 7 7 7 7 7 7 7 7 7 7 7 7 7.7 7.7 8.1 8.1 7.6 7.6 7.5 7.5 7.1 7.1 7.0 7.0 Cedar Bayou at US 90 Southwest of Dayton W C W P W C W P W C W C W P W C W P W C W P Te»t Specie* O Csrfedapnnfe tfuMa P= PfaMonaaw ammeau 0 3 0 7 0 100 7 20 3 10 43 0 Q 3 Q o Q 10 Q o Q 0 (•) o Q 10 Q 10 Q 10 Q 7 ® 20.1 17.9 18.4 19.2 15.7 17.9 16 17.1 16.2 16.2 S= Cyprinodon venogeha (•) Significant Difference D=Dao/ini.ou»M I) No SkmMeant Difference o o o o o Significant Effect* Crustacean* - mortality. Rmh . mortality, taratnoanicitv or ah 7 7 7 7 7 3 7 7 7 7 7 nnfm*il mvimmina 7.0 7.0 7.3 7.3 6.9 6.7 6.7 7.8 7.8 7.8 7.8 138 199 86 179 86 179 771 < 0.1 489 < 0.1 489 < 0.1 TNRCC 12030105022 32*4679* 96*4?18* 121 150 518 < 0.1 TNRCC 12030102007 32*45W 97*1771* 146 172 346 < 0.1 TNRCC 12030102004 32*45'46* 96*59'4r 154 191 154 191 186 154 186 154 141 182 141 182 143 128 143 128 127 192 127 192 188 217 188 217 780 < 0.1 780 < 0.1 494 < 0.1 494 < 0.1 746 02 746 02 470 < 0.1 470 < 0.1 477 < 0.1 477 « 0.1 586 < 0.1 586 < 0.1 7NRCC 12040203002 29*5870* 94*59'08* 77 119 77 119 166 258 166 258 74 106 90 100 90 100 158 194 158 194 122 158 122 158 362 < 0.1 362 < 0.1 593 < 0.1 593 < 0.1 298 < 0.1 314 < 0.1 314 < 0.1 468 < 0.1 468 < 0.1 558 < 0.1 558 < 0.1 0.4 0.5 0.5 Dallas TX 0.6 Tamnt TX 0.2 Dallas TX 02 02 0.1 0.1 0.6 0.6 02 02 0.5 0.5 0.6 0.6 Liberty TX 0.7 0.7 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.2 0.2 YPF « average number of young per female • applicable only to C*enxKiepnnM OUDM. behaviour Wolnht . •nnlleahU onlv to fhh kuval uiivkial and nmuith hHt 28 ------- Station Number Date of Collection 1006.0200 28-Oct-91 28-Apr-92 28-Apr-92 26-Aug-92 1007.9200 14-Od-92 13-Jan-93 07-Apr-93 14-Jul-93 15-Oct-93 11-Jan-94 22-Apr-94 1113.0020 30-Sep-92 14-Dec-92 16-Mar-93 09-Jun-93 14-Sep-93 07-Dec-93 Waterbody Description Sample Test Type Species Test Percent Effected Control Test Percent significant Test Control Significant weight Effected Effects YPF YPF DrflnYPF (mg) Control Significant Test Weight Difln Duration (mg) Weight (Days) (S.U.) Houston Ship Channel at Greens Bayou W S W C W P W S 0 10 7 23 3 Q 0 Q 15.6 22.3 (§) 7 O 9 7 7 9 7.7 6.8 6.8 7.4 Vlnce Bayou at Confluence with Houston Ship Channel S S W S S S W S S S W S S S 100 10 100 0 13 7 17 7 (S) 3 Q 7 Q 3 Q 0 Q 7 O 6 9 6 9 9 9 9 7.4 6.8 6.8 7.1 7.1 7.2 7.2 Armand Bayou Tidal At Lower Mud Lake 1.4 Km Upstream of NASA 1 Bridge W S S S W S S S W S S S 17 10 3 23 0 10 3 Q 0 Q 7 Q 3 (•) 3 Q 3 O 8 9 9 9 9 9 8.6 7.3 7.3 7.3 7.2 7.6 Agency EPA Reach Latitude Longitude County State Alkalinity (mgfl.) (mgfl.) Conductivity (umhos/cm) Total Chlorine (mgfl.) Total Ammonla-N (mgfl.) TWRCC 12040104015 29'44'46" 95'10'Or 122 82 82 128 0 89 89 2000 12500 302 302 16500 TNRCC 12040104017 29 174 110 172 103 131 167 166 2000 128 1984 103 30300 460 17300 485 21300 7760 27500 < 0.1 < 0.1 < 0.1 < 0.1 •43-31- 95 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 1.0 1.2 1.2 0.4 •13'29" 12.0 3.8 25.2 2.0 1.4 9.6 15.6 TNRCC 12040204008 29'34127" 95*04'15" 114 93 89 112 99 143 2000 927 2869 1230 16240 30500 6820 24300 21800 24500 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 0.84 1.4 0.8 3.6 4.8 8.4 Unionized Ammonla-N (mgfl) Harris TX Harris TX < 0.1 Harris TX < 0.1 1200.7820 Bryan Municipal Lake near Dam Spillway TNRCC 30'38'24" 96'2V33- Brazos TX 01-Oct-91 01-Oct-91 18-Feb-92 18-Feb-92 21-Jul-92 21-Jul-92 23-Feb-93 23-Feb-93 02-Aug-93 02-Aug-93 30-Nov-93 W W W W W W W W S S W C P C P C P C P C P C 0 0 10 7 0 3 0 7 20 13 0 0 ( 7 ( 0 ( 7 ( 10 ( 3 ( 0 ( 0 ( 0 (j 7 ( 0 ( ~) 10.3 1 J 18 J J 18.5 J J 22.2 J j) s J J 18.3 13.6 18.1 18.2 20.8 19 17.7 (§) O O O (§) O 7 7 7 7 7 7 7 7 7 7 7 7.6 7.6 8.0 8.0 8.0 8.0 7.8 7.8 7.9 7.9 7.2 145 145 94 94 178 178 154 154 209 209 152 74 74 75 75 61 61 77 77 128 128 63 493 493 339 339 694 694 604 604 608 608 951 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 « 0.1 < 0.1 < 0.1 0.6 0.6 0.2 0.2 0.5 0.5 0.5 0.5 13.2 13.2 0.8 Sample Types Test Species Significant Effects W=Water C= Coriodaphnifdubi* S= Cyprinodon vmymtiu (S) Significant Difference Crustaceans - mortality. S=Sediment P= Pimephmlos promotes D= Daphnit pub* Q No Significant Difference Fish - mortality, teratogenicity, or abnormal swimming behaviour. YPF • average number of young per female - applicable only to Carfcdepnnfe dubla. Weight • applicable only to fish larval survival and growth test 29 ------- Station Number Waterbody Description Agency EPA Reach Latitude Longitude County State Test Control Test Control Significant Test Total Total Unionized Sample Test Percent Percent Significant Test Control Significant Weight Weight Dtfln Duration pH Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-N Date of Collection Type Species Effected Effected Effects YPF YPF DHInYPF (mg) (mg) Weight (Days) (S.U.) (mgfl.) (mg/L) (umhos/cm) (mg/L) (mg/L) (mgfl) 30-NOV-93 1200.7822 20-Mar-90 20-Mar-90 14-Aug-90 14-Aug-90 27-Nov-90 27-Nov-90 04-Feb-91 21-May-91 21-May-91 20-May-92 20-May-92 1200.7830 12-Feb-90 12-Feb-90 20-Mar-90 20-Mar-90 14-Aug-90 14-Aug-90 27-Nov-90 27-Nov-90 08-Jul-91 08-Jul-91 01-0ct-91 01-Od-91 18-Feb-92 18-Feb-92 21-Jul-92 21-Jul-92 23-Feb-93 23-Feb-93 02-Aug-93 02-Aug-93 Sample Types tA/-U/a*Ar W P 7 3 u 7 7.2 Bryan Municipal Lake Malnbody W W W W W W W W W W W C P c P c P c c P c P 10 0 0 3 0 5 0 0 0 0 3 0 0 0 3 0 0 10 0 7 0 7 O 13.4 Q Q 17.5 o Q 19.4 (~) Q 19.7 Q 17.5 Q Q 21.2 o 17.7 17.2 16.7 15.5 14.8 16.2 (§) O o 8 O 7 7 7 7 7 7 7 7 7 7 7 8.1 8.1 9.3 9.3 8.3 8.3 5.9 8.9 8.9 7.7 7.7 Flnfeathor Lake near Dam Spillway W W W W W W W W W W W W W W W W W W S S Test Species f~s rmrif*t**h~ c P c P c P c P c P c P c P c P c P c P Im rtuttim 100 27 10 3 50 0 0 5 0 3 10 15 0 13 60 7 0 10 100 17 0 0 0 0 0 3 0 0 0 3 0 7 0 7 10 3 0 0 0 7 _r *.._ © (•) Q 18.5 Q Q 5.5 Q Q 18.2 Q Q 16.1 (™) Q 18.3 Q Q 16.3 (~) (•) 16.2 (J Q 21.3 o X o nBn cinntfiMn* ruw. 17.7 17.2 16.7 16.3 13.6 18.1 18.2 20.8 o o o 0 o o o o Significant Effect* fVi tftmf^mnm . nvufalifu 2 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 1 7 8.1 8.1 7.9 7.9 9.2 9.2 8.3 8.3 8.6 8.6 8.2 8.2 8.5 8.5 8.2 8.2 7.9 7.9 8.3 8.3 152 TNKCC 223 223 193 193 189 189 19 146 146 150 150 TNRCC 326 326 315 315 262 262 141 141 221 221 189 189 154 154 146 146 144 144 239 239 VDC _ etuAMj 63 951 < 0.1 0.8 30'38'2r 96*21'3r Brazos 7X 80 444 80 444 65 476 65 476 76 737 76 737 48 179 68 535 68 535 61 488 61 488 12070103034 78 952 78 952 65 776 65 776 74 857 74 857 88 1273 88 1273 75 901 75 901 77 818 77 818 71 551 71 551 41 655 41 655 101 662 101 662 194 688 194 688 < 0.1 < 0.1 < 0.1 < 0.1 « 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 SO'SSW 96*2 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 « 0.1 < 0.1 < 0.1 < 0.1 1 1 1.2 1.2 0.2 0.2 0.5 0.5 0.5 0.6 0.6 0.6 0.6 2-16- Braros TX 0.6 0.6 1.2 1.2 0.2 0.2 0.6 0.6 0.4 0.4 0.7 0.7 0.4 0.4 0.7 0.7 0.6 0.6 14.4 14.4 *L_ *»lu«H /"Vu^J...*!*!^ jJllJkW (_) No Significant Difference Fish - mortality, teratogenicity, or abnormal swimming behaviour. Weight • applicable only to ftth larval survival and growth test 30 ------- Station Number Waterbody Description Agency EPA Reach Latitude Longitude County State Date of Collection Continued... 30-Nov-93 30-Nov-93 1200.7832 21-May-91 21-May-91 20-May-92 20-May-92 1200.7834 Sample Type Test Species Test Percent Effected Control Percent Effected Test Control Significant Test Significant Test Control Significant Weight Weight DH In Duration pH Effects YPF YPF DlfinYPF (mg) (mg) Weight (Days) (S.U.) Total Total Unionized Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-N (mg/L) (mgfl,) (umhos/cm) (mgfl.) (mg/L) (mgfl) Flnfeather Lake near Dam Spillway '—* W W C P 0 0 0 3 819.4 17.7 O . 7 7 7.0 7.0 FlnfeatherLake Main Body W W W W C P C P 100 3 20 10 0 7 0 7 ® Q Q 20.5 16.2 O Q 4 7 7 7 8.2 8.2 8.0 8.0 Flnfeather Lake Headwater 154 117 154 117 687 < 0.1 687 < 0.1 7WRCC 12070103034 30*39'01" 96'2Z20T 198 76 198 76 144 82 144 82 837 < 0.1 837 « 0.1 470 < 0.1 470 < 0.1 TWRCC 12070103034 SO'SffH" 96'22'23" 0.4 0.4 Brazos 0.5 0.5 0.6 0.6 Brazos TX TX 04-Feb-91 W 100 8.0 64 56 174 0.1 0.5 1218.0270 Nolan Creek 200 Yards Above Bell County WCID #1 Outfall 2S-Feb-90 W C 26-Feb-90 W P 27-Mar-90 W C 27-Mar-90 W P 04-Jun-90 W C 04-Jun-90 W P 07-Aug-90 W C 24-Sep-90 W C 24-Sep-90 W P 12-Feb-91 W C 28-May-91 W C 28-May-91 W P 15-Jul-91 W C 15-Jul-91 W P 0 0 0 3 40 13 0 60 10 0 10 0 0 10 10 Q 0 Q 10 Q 7 Q 10 Q 7 Q 10 Q 10 ® 3 Q 0 Q 0 Q 0 Q o Q 7 O 15.2 15.8 5.5 17.3 0 15.9 11.5 14 14.1 O 14.7 O 14.2 ® 15.5 Q 17.2 ® 16.6 Q 13.2 O 15.9 O 7 7 7 7 7 7 7 7 7 7 7 7 7 7 8.1 8.1 8.0 8.0 8.0 8.0 7.9 7.8 7.8 8.1 7.8 7.8 7.4 7.4 1300.0710 Unnvtlte Bayou at CR S21, 230 Meters Upstream from Caney Creek Confluence 06-Apr-89 W C 06-Apr-89 W P 0 100 0 Q 0 ® 2.5 16.4 ® 7 7 8.5 8.5 1300.0717 Unnvtlte Bayou at CR 321 (Boons Road) 25-Aug-92 W C 25-Aug-92 W P 23-Sep-92 W P Sample Types Test Species 0 40 3 o Q 3 ® 10 O 1.6 14.3 ® Significant Effects 7 7 7 7.2 7.2 8.3 TNRCC 204 204 211 211 94 94 128 159 159 196 180 180 168 168 TNRCC 110 110 TNRCC 102 102 196 12070201062 31<06128" 97'42'12- 234 234 245 245 102 102 167 190 190 237 200 200 204 204 523 523 526 526 239 239 289 280 280 531 487 487 495 495 12090402006 21 273 273 3020 3020 < 0.1 < 0.1 < 0.1 < 0.1 « 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 }«5T08- 95M2'3r < 0.1 « 0.1 12090402006 297 297 89 2500 2500 983 < 0.1 < 0.1 < 0.1 Be» TX 0.7 0.7 0.8 0.8 0.7 0.7 0.5 0.5 0.5 0.2 0.3 0.3 0.4 0.4 Matagorda TX 3.4 1.9 3.4 1.9 Matagorda TX 3.5 3.5 3.1 W=Water O Coriodaphnla dubtt S= Cyprinodon vtriegatus (•) Significant Difference Crustaceans - mortality. S=Sediment P= Pimephfba pmmelts D= Oipnnie pufex O No Significant Difference Fish - mortality, teratogenicity, or abnormal swimming behaviour. YPF - average number of young per female - applicable only to Cfriodtphnm dubit. Weight - applicable only to fish larval survival and growth test 31 ------- Station Number Watetbody Description Sample TMt Type Species Test Percent Effected Date of Collection Type Species Effected Effected Continued.- Unnville Bayou at CK 321 (Boone Road) Control Tnt Control Significant TMt Percent significant Tnt Control Significant W*4ght WalgM Uf In Duration pH Effects YPF YPF DM In YPF (mg) (mg) WalgM (Days) (S.U.) Agency EPA Reach Latitude Longitude County State Total Total Unionized Alkalinity Hardnan Conductivity Chlorine Amnwnla-N Ammonla-N (tng/L) (mgn.) (umhos/cm) (mg/L) (mj/L) (mg/l) 23-Sep-92 M-Oec-93 06-Dec-93 06-Dec-93 06-DOC-93 S W W S S C C P C P 0 0 7 10 7 0 ( 0 ( 3 ( 0 ( 3 ( J 20.7 J 19.2 J J 19.4 J 21.3 16.8 19.7 0 o o 7 7 7 7 7 8.3 6.7 6.7 7.4 7.4 196 89 89 146 146 89 100 100 71 71 983 429 429 689 689 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 3.1 0.5 0.5 3.6 3.6 1300.0726 Unnvtlto Bayou at CR 324 (Haaoma Road) TNRCC 12090402006 29TJ734- 9S*46"54> Matagortta TX 24-Apr-90 24-Apr-90 16-Jul-90 10-Oct-90 21-Jan-91 03-Apr-91 03-Apf-91 23-Jul-91 23-Jul-91 01-Od-91 01-Oct-91 02-Dec-91 02-Dec-91 24-Mar-92 24-Mar-92 07-Jul-92 07-Jul-92 25-Aug-92 25nAug-92 23-Sap-92 23-Sap-92 23-Sep-92 23-Sep-92 15-DBC-92 15-Oec-92 17-Mar-93 17-Mar-93 15-Jun-93 15-Jun-93 W W W W W W W W W W W W W W W W W W W W W S S W W W W S S C P C C C C P C P C P C P C P C P C P C P C P C P C P C P 0 50 0 60 0 100 50 80 90 0 100 100 100 0 100 20 100 10 100 0 100 0 53 0 10 0 7 20 0 0 C 7 (J 0 C o Q o C 0 ( 3 ( 0 ( 7 ( 0 C 7 ( 0 ( 3 ( o C 7 ( 10 ( 7 ( o C 3 ti o C 10 6 o C 10 ^ o C 3 C 0 £ 7 £ o C 7 Q ~) 92 P } 5.7 » o } 16.6 ; ; ) 5.5 5 J 4.9 5 ; 5 } 0.4 5 ) 3.3 5 J 2.3 ?) J 3.4 9 J 25.6 P J 17.5 ; J 20.5 ; ) 21.3 5 18.1 (•) 14.6 ® 16.5 ® 15.7 O 15.2 ® 13.6 (•) 17.6 (§) 13.1 (•) 14.3 (§) 21.3 (§) 21.3 O 18.7 O 19.7 O 18.3 O 7 7 7 7 7 1 7 7 7 7 5 1 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7.6 7.6 7.9 7.4 6.6 7.8 7.8 7.4 7.4 7.5 7.5 7.8 7.8 8.0 8.0 72 7.2 7.6 7.6 7.3 7.3 8.1 8.1 7.6 7.6 7.4 7.4 6.8 6.8 150 150 202 176 81 225 225 221 221 212 212 214 214 196 196 100 100 108 108 150 150 216 216 95 95 118 118 177 177 282 282 244 370 84 241 241 267 267 291 291 237 237 253 253 277 277 268 268 301 301 69 69 132 132 133 133 179 179 2240 2240 3520 3920 189 2270 2270 2340 2340 2040 2040 2550 2550 2400 2400 2660 2660 2300 2300 3420 3420 870 870 451 451 260 260 527 527 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 0.1 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 6.0 6.0 12 12.0 0.5 18.0 16.0 15.6 15.6 7.7 7.7 7.7 7.7 13.0 13.0 1.8 1.8 4.8 4.8 0.4 0.4 5.3 5.3 0.2 0.2 0.5 0.5 0.2 0.2 Sampla Tvpn Tnt Spacln W=Water C= Ceriodaphnim dubi* S= Cyprinodcn vtritgttus S=Sediment P= HmapAafea prometoa D= Daphnlm puhx (•) Significant Difference O No Significant Difference Significant Effects Crustacean* - mortality. Fish - mortality, teratogenicity, or abnormal swimming behaviour. YPF - average number of young per female • applicable only to Cariodmphnim dubi*. Weight - applicable only to fish larval survival and growth test 32 ------- Station Number Waterbody Description Agency EPA Reach Latitude Longitude County State Test Samplt Te»t Percent Date of Collection Type Species Effected Control Percent Significant Test Effected Effects YPF Continued... LJnnvtlte Bayou at CR 324 (Hasema Road) 14-Sep-93 WC 0 0 Q 14-Sep-93 W P 7 06-Dec-93 W C 0 06-Dec-93 W P 7 06-Dec-93 S C 0 06-Dec-93 S P 10 • Q i R 3 O 17.3 19.8 19 Test Control Significant Test Control Significant Weight Weight Dtfln Duration pH YPF DiflnYPF (mg) (ing) Weight (Days) (S.U.) 18.8 16.8 19.7 o o o 7 7 7 7 7 7 7.0 7.0 7.0 7.0 7.5 7.5 1300.0750 UnnvtOe Bayou at SH35, West of Old Ocean 25-Aug-92 W C 0 25-Aug-92 W P 3 23-Sep-92 S C 0 23-Sep-92 S P 3 06-Dec-93 W C 0 06-Dec-93 W P 3 06-Dec-93 S C 0 06-Dec-93 S P 7 o Q 3 Q o Q 10 Q 0 Q 3 Q 0 Q 3 O 13.2 23.9 20.9 20.7 14.3 21.3 16.8 19.7 o 0 o o 7 7 7 7 7 7 7 7 7.1 7.1 7.6 7.6 6.6 6.6 8.2 8.2 1302.0100 San Bernard River Bridge on FM 442 Northeast of Bollng 24-Apr-90 W C 0 24-Apr-90 W P 10 16-Jul-90 W C 0 10-Oct-90 W C 0 21-Jan-91 W C 0 03-Apr-91 W C 0 03-Apr-91 W P 3 23-Jul-91 W C 0 23-Jul-91 W P 20 0 Q 7 Q 0 Q o Q o Q 0 Q 3 Q 0 Q 7 O 18.9 10.4 17.7 20.1 20.2 19.6 18.1 14.6 16.5 15.7 17.3 15.2 o (•) Q Q o 0 7 7 7 7 7 7 7 7 7 7.5 7.5 8.4 7.8 6.3 7.7 7.7 6.7 6.7 1305.0075 Caney Creek at Bridge 200 Yards Below Unnvllle Bayou Confluence 06-Apr-89 W C 0 06-Apr-89 W P 7 24-Apr-90 W C 0 24-Apr-90 W P 3 16-Jul-90 W C 0 01-Oct-91 W C 10 01-0ct-91 W P 7 Sample Types Test Species 0 Q o Q 0 Q 7 Q 0 Q 0 Q 7 O 17.2 19.3 12.3 15.1 16.4 18.1 14.6 13.6 o o 8 Significant Effects 7 7 7 7 7 7 7 8.7 8.7 8.0 8.0 8.1 7.5 7.5 Total Total Unionized Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-N (mg/L) (mg/L) (umnosfcm) (mg/L) (mg/L) (mg/l) 160 160 114 114 127 127 7NRCC 130 130 204 204 124 124 116 116 TNRCC 67 67 144 177 28 225 225 90 90 TNRCC 219 219 144 144 148 174 174 300 3870 300 3870 171 1268 171 1268 108 698 108 698 12090402006 158 236 158 236 179 501 179 501 150 299 150 299 179 523 179 523 12090401002 84 167 84 167 232 884 268 805 40 77 227 689 227 689 142 378 142 378 12090402006 252 1605 252 1605 265 1268 265 1268 207 1091 238 994 238 994 0.1 0.1 < 0.1 < 0.1 < 0.1 < 0.1 29'04'10" 95M7-20" < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 29*1 ff4r K*SS3S' < 0.1 < 0.1 < 0.1 < 0.1 « 0.1 < < 0.1 < 0.1 < 0.1 < 0.1 28-5T08' 95M2'32" < 0.1 < 0.1 < 0.1 < 0.1 « 0.1 < 0.1 < 0.1 ,-*- . 1.0 1.0 0.6 0.6 1.9 1.9 Matagorda 0.4 0.4 1.3 1.3 0.2 0.2 1.1 1.1 Wharton 0.5 0.5 0.4 0.5 0.1 0.2 0.2 0.5 0.5 Matagorda 1.6 < 1.6 < 0.2 0.2 0.6 0.8 0.8 TX TX TX 0.1 0.1 W=Water C= Coriodtphnit dubla S= Cyprinodon varlegatm (•) Significant Difference S=Sediment P= Pimaphalat promotes D= Dtphnia pulex Q No Significant Difference Crustaceans - mortality. Fish - mortality, teratogenicity, or abnormal swimming behaviour. YPF - average number of young per female • applicable only to Ceriodaphnia dubla. Weight • applicable only to fish larval survival and growth test 33 ------- Station Number Watertody Description Agency EPA Reach Latitude Longitude County State Sample Ttst Date of Collection Type Species Test Percent Effected Control Test Control Significant Test Percent significant Ttst Control Significant Wtlght Wtlght Off In Duration pH Efftcttd Effects YPF YPF DtflnYPF (mg) (mg) Weight (Days) (S.U.) Alkalinity Hardness (mg/L) (mg/L) Total Total Unionized Conductivity Chlorine Ammonla-N Ammonla-N (umhos/cm) (mg/L) (mg/L) (mg/l) Continued., Caney Creek at Bridge 200 Yards Below Unnvllle Bayou Confluence 02-Dec-91 W C 02-Dec-91 W C 24-Mar-92 W C 24-Mar-92 W P 07-Jul-92 W C 07-Jul-92 W P 22-Sep-92 W C 22-Sep-92 W P 15-Oec-92 W C 15-Dec-92 W P 17-Mar-93 W C 17-Mar-93 W P 15-Jun-93 S C 15-Jun-93 S P 14-S«p-93 W C 14-Sep-93 W P 09-Dec-93 S C 09-Dec-93 S P 0 0 0 3 10 3 0 7 0 3 0 7 0 3 0 0 0 3 o O 3 Q 0 Q 7 Q 10 Q 7 Q o Q 10 Q 0 Q 3 Q o Q 7 Q o Q 7 Q 0 Q o Q o Q 3 O 17.5 16.9 19.6 17.6 15.3 13.1 21 21.3 18.5 18.7 20.3 19.7 20.7 18.3 20.5 18.8 20.8 19.4 O o o 0 0 o o o o 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7.9 7.9 8.1 8.1 8.3 8.3 7.8 7.8 7.0 7.0 7.0 7.0 7.5 7.5 7.2 72 7.3 7.3 1305.0100 Caney Creek above Tidal at FM 52! 06-Apr-89 W C 06-Apr-89 W P 0 3 o Q o O 18.6 16.4 o 7 7 8.8 8.8 1412.0140 Colorado River at SH163 In Colorado City 19-Apr-93 W C 19-Apr-93 W P 19-Apr-93 S C 19-Apr-93 S P 16-Aug-93 W C 15-Aug-93 W P 16-Aug-93 S C 16-Aug-93 S P 10 13 0 3 100 17 0 3 0 Q 3 Q o Q 10 Q 0 ® 3 Q 0 Q 3 O 3 19.6 20.7 19.1 21 18.2 ® o 0 7 7 7 7 1 7 7 7 7.9 7.9 7.2 7.2 7.8 7.8 6.9 6.9 1421.0470 North Oincho River 400 Meters Downstream of Johnson Dam In San Angelo 15-0ct-91 W C 15-0ct-91 W P Sample Types Test Species 100 0 Significant Effects 2 7 7.9 7.9 99 99 212 212 132 132 192 192 85 85 72 72 184 184 179 179 196 196 TNRCC 203 203 TNRCC 200 200 214 214 264 264 198 198 TNRCC 250 250 174 174 253 253 184 184 203 203 136 136 97 97 183 183 238 238 188 188 1191 1191 1090 1090 600 600 818 818 362 362 140 140 505 505 1114 1114 621 621 12090402006 189 189 529 529 12080002017 940 940 155 155 256 256 161 161 393 393 4420 4420 705 705 12370 12370 1155 1155 1428 1428 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 « 0.1 < 0.1 < 0.1 < 0.1 < 0.1 02 02 02 02 28'56-26" 95'43118" < 0.1 < 0.1 1.8 1.8 1.7 1.7 0.4 0.4 0.4 0.4 0.5 0.5 0.5 0.5 0.2 0.2 0.4 0.4 0.5 0.5 Matagorda TX 0.4 < 0.1 0.4 < 0.1 32'22-30- lOO'SrSO- Mitchell TX < 0.1 « 0.1 < 0.1 < 0.1 0.2 0.2 < 0.1 < 0.1 3i'3i-2r ^oa•22•3tt < 0.1 < 0.1 0.2 0.2 1.2 1.2 1.9 1.9 1.2 1.2 Tom Green TX 0.7 0.7 W=Water C= Ceriodaphni* dubi» S= Cyprinodon vtriogatus (•) Significant Difference Crustaceans - mortality. S=Sediment P= Pimephfha promotes D= Dtp/inn puhx (_) No Significant Difference Fish - mortality, teratogenicity. or abnormal swimming behaviour. YPF - average number of young per female • applicable only to Ceriodmphni* duA/a. Weight - applicable only to fish larval survival and growth test 34 ------- Station Number Waterbody Description Agency EPA Reach Latitude Longitude County State Sample Date of Collection Type Test Test Percent *p*cw» Efrecno Control Percent Effected Test Control Significant Test Significant Test Control Significant Weight Weight Dtf In Duration pH Effects YPF YPF DlflnYPF (mg) (mg) Weight (Days) (S.U.) Total Alkalinity Hardness Conductivity Chlorine (mg/L) (mgfl.) (umhos/cm) (mg/L) Total Unionized Ammonla-N Ammonla-N (mg/L) (mgfl) Continued... North Concho River 400 Meters Downstream of Johnson Dam In San Angelo «... 29-Jan-92 W 29-Jan-92 W 21-Apr-92 W 21-Apr-92 W 28-Jul-92 W 19-Apr-93 W 19-Apr-93 W 19-Apr-93 S 19-Apr-93 S 16-Aug-93 W 16-Aug-93 W 16-Aug-93 S 16-Aug-93 S C 0 P 3 C 0 P 13 C 0 C 0 P 10 C 0 P 0 C 0 P 7 C 0 S 0 10 0 0 7 0 0 3 0 10 0 3 0 3 Q 16.3 16.2 (~) Q 20.6 16.8 Q Q 18.2 17.7 Q 21.6 19.6 Q Q 21.9 19.1 o Q 18 17.5 o Q 15.8 18.2 Q O o 8 O 0 ® 7 7 7 7 7 7 7 7 7 7 7 7 7 7.9 7.9 7.1 7.1 8.2 8.1 8.1 7.1 7.1 8.2 8.2 7.5 7.5 1429.0800 Town Lake at Longhom Dam 04-Feb-91 W 04-Feb-91 W 05-Nov-91 W 05-Nov-91 W 04-May-92 W 04-May-92 W 25-Aug-92 W 25-Aug-92 W C 0 P 7 C 0 P 0 C 40 P 0 C 0 P 13 0 10 0 3 0 3 0 3 O 15.9 15 (~j Q 14.1 12 o ® 2.8 17.6 Q Q 11-3 14.3 Q 0 o ® ® 7 7 7 7 7 7 7 7 7.6 7.6 8.2 8.2 8.1 8.1 7.6 7.6 1429.TMP1 Waller Creek at 2nd Street In Austin 04-Sep-91 W 04-Sep-91 W C 0 P 15 0 0 0 7.1 14.7 o ® 7 7 7.4 7.4 1429.TMP2 Waller Creek @ 19th Street In Austin 04-Sep-91 W 04-Sep-91 W C 0 P 5 0 0 8 " '" o 7 7 7.8 7.8 1 429.TMP3 Waller Creek Q 38th Street In Austin 04-Sep-91 W 04-Sep-91 W C 0 P 15 0 0 8 ™ 1" o 7 7 7.7 7.7 143 213 143 213 100 204 100 204 246 467 300 631 300 631 250 194 250 194 205 384 205 384 245 231 245 231 613 613 482 482 1563 1549 1549 464 464 1260 1260 646 646 7A/RCC 12090205004 143 178 143 178 164 238 164 238 150 196 150 196 164 215 164 215 7NRCC 126 172 126 172 7NRCC 126 197 126 197 7NRCC 145 221 145 221 502 502 614 614 437 437 425 425 495 495 513 513 493 493 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 0.1 0.1 0.1 0.1 2.3 2.3 0.5 0.5 0.2 0.8 0.8 3.6 3.6 0.2 0.2 10.8 10.8 SO'lS'OO" 97*42'48" < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 « 0.1 « 0.1 < 0.1 « 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 0.4 0.4 0.4 0.4 0.5 0.5 0.5 0.5 0.7 0.7 0.9 0.9 0.9 0.9 "~ Travis TX Travis TX < 0.1 < 0.1 Travis TX Travis TX Sample Types Test Species Significant Effects W=Watec C= Coriodaphnia dubia S= Cyprinodon variogali/3 ® Significant Difference Crustaceans - mortality. S=Sediment P= Pimephalea pmmolaa 0= Daphnia pub* (_) No Significant Difference Fish • mortality, teratogenicrty, or abnormal swimming behaviour. YPF • average number of young per female - applicable only to Ceriodaphnla dub/a. Weight - applicable only to fish larval survival and growth test 35 ------- Station Number Waterbody Description Agency EPA Reach Latitude Longitude County State Test Control Test Control Significant Test Sample Test Percent Percent Significant Test Control Significant Weight Weight Dtfln Duration pH Alkalinity Hardness Conductivity Date of Collection Type Species Effected Effected Effects YPF YPF DtflnYPF (mg) (mg) Weight (Days) (S.O.) (mg/L) (mgfl.) (umhosfcm) 1803.0100 20-Jun-89 20-Jun-89 1803.0102 20-Jun-89 20-Jun-89 1803.0950 20-Jun-89 20-Jun-89 1906.0230 06-Jun-89 OS-Jun-89 11-Jun-89 11-D«c-91 11-Oec-91 24-Feb-92 24-Feb-92 12-May-92 12-May-92 07-Jul-92 07-Jul-92 OI-Dec-92 OI-Dec-92 01-Dec-92 01-Dec-92 17-May-93 17-May-93 17-May-93 17-May-93 12-Jul-93 12-Jul-93 12-Jul-93 Sample Types WsWatsr Guadalupe River at SH 175 South of Victoria W D 3 7 Q W P 17 7 O 2 8 8.0 8.0 Guadalupe River 0.5 Mile above SH 175 Bridge South of Victoria W D 3 7 Q W P 3 7 O 2 8 8.0 8.0 Guadalupe River 0.5 Mile below SH 175 South of Victoria W D 10 7 Q W P 13 7 O 2 8 8.0 8.0 Leon Creek at Ruiz Ranch Low Water Crossing. I.ISKm Downstream of Loop 13 South of San Antonio WC 0 0 Q W P 0 5 Q S D 0 5 Q W C 50 0 ® W P 27 3 W W C 0 0 Q W P 15 7 Q W C 0 10 Q W P 7 3 Q W C 10 10 Q W P 7 7 Q W C 0 10 Q W P 37Q S C 0 0 Q S P 3 3 Q w c o o Q W P 73Q S C -0 0 Q S P 700 W C 000 W P 370 S C 0 10 0 Test Spades 21.6 20.7 10.2 19 13.5 15.9 12.4 19.1 14.1 13.1 17.1 17.4 21.6 18.7 21.6 18.7 21.5 20.3 21.5 19.1 21.9 18.4 P= Pimeohmlas ommolma D* Daonnfe Bute* O No Sianificant Difference O ® (§) (§) o o o o o o 0 Sianificant Effect! Crustaceans mortality Fish • mortality, teretooenicitv. or abnormal n 7 7 2 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7.8 7.8 8.6 8.1 8.1 7.8 7.8 7.8 7.8 7.8 7.8 7.6 7.6 7.7 7.7 7.0 7.0 6.7 6.7 7.5 7.5 7.4 TNRCC 150 150 TNRCC 143 143 TNRCC 146 146 TNRCC 296 296 235 235 189 189 256 256 262 262 245 245 150 150 237 237 173 173 230 230 158 Total Chlorine (mgfl.) Total Unionized Ainntonla-N Amntonla^i (mgfl.) (mgfl) 28M5-10- 97'0ff30- Victoria 148 148 148 148 148 148 342 342 328 328 313 313 < 0.1 < 0.1 < 0.1 * 0.1 < 0.1 < 0.1 12100302005 29-21-20" 98' 212 212 245 245 277 277 335 335 306 306 335 335 240 240 282 282 171 171 250 250 158 1002 1002 462 755 755 475 475 710 710 597 597 590 590 453 453 751 751 505 505 796 796 490 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 « 0.1 t 0.1 t 0.1 « 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 0.8 0.8 Victoria < 0.1 < 0.1 Victoria 0.7 0.7 '34-38- Bexar 1.2 1.2 3.6 4.8 4.8 22 2.2 0.3 0.3 0.4 0.4 0.4 0.4 1.5 1.5 0.2 0.2 1.3 1.3 1.2 1.2 1.2 TX TX TX TX 0.3 0.3 VDC _ •! !•«••• «inmL -. m* uuuui * »- --•ll..»lil» juttu 4>% f*~rin rim ntinlm tttJttm M behaviour i rr - «iwf«yv MMIIIUVI wi yvmiy pwi IWIIMIV • «pi/iiv««utv winy vw w*n iww«Bf. mow ww«. 36 ------- Station Number Watertody Description Test Sample Test Percent Date of Collection Type Specie* Effected Control Test Control Significant Test Percent significant Test Control Significant Weight Weight Off In Duration Effected Effects YPF YPF DtfinYPF (mg) (mg) Weight (Days) PH (S.U.) Agency EPA Reach Latitude Longitude County State Total Total Unionized Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-N (man.) (man.) (umhosfcm) (mg/L) (mgA.) (mgA) Continued.- Leon Creek at Ruiz Ranch Low Water Crossing. I.SSKm Downstream of Loop 13 South of San Antonio .,**. , 12-Jul-93 S P 10-May-94 W C 10-May-94 W P 10-May-94 S C 10-May-94 S P 0 0 0 0 7 3 O 0 Q 3 O 0 Q 7 O 20.7 20.9 19.2 18.4 O o 7 7 7 7 7 7.4 6.9 6.9 7.7 7.7 1906.0240 Leon Creek Approximately 25 Yards Below Kelly AFB Outfall 001 06-Jun-89 W C 06-Jun-89 W P 11-Jun-89 S D 40 IS 0 0 (•) 5 Q s O 13.2 20.7 ® 7 7 2 7.9 7.9 8.7 1906.0245 Leon Creek at Low Water Crossing Immediately Upstream of Kelly AFB Outfall 001 06-Jun-89 W C 06-Jun-89 W P 11-Jun-89 S D 0 10 0 0 Q 5 Q 5 O 21.2 20.7 0 7 7 2 8.1 8.1 8.2 1911.0300 San Antonio River at FM 1604 West of Elmendorf 28-Feb-90 W C 28-Feb-90 W P 05-Jun-90 W C 05-Jun-90 W P 31-Jul-90 W C 31-Jul-90 W P 29-Aug-90 W C 13-Nov-90 W C 19-Feb-91 W C 20-May-91 W C 20-May-91 W P 01-Dec-92 W C 01-Dec-92 W P 01-Dec-92 S C 01-Dec-92 S P 12-Jul-93 W C 12-Jul-93 W P 12-Jul-93 S C 12-Jul-93 S P Sample Types Test Species 0 7 0 0 0 7 0 0 0 0 0 0 7 0 0 0 0 10 10 10 Q 0 Q 10 Q 7 Q 0 Q 3 Q 0 Q 0 Q 0 Q o Q 7 Q 10 Q 7 Q 0 Q 3 Q 0 Q 7 (~) 10 Q 3 O 15.8 14.1 15.5 14.2 20.1 19.5 18.2 14.4 18.5 17.1 19.6 17.2 16.4 15.2 18.4 17.4 20.5 18.7 19.3 19.1 16.6 18.4 0 o o o o o o o o 0 0 Significant Effects 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 8.1 8.1 8.3 8.3 8.2 8.2 8.1 7.8 8.1 8.2 8.2 8.0 8.0 7.8 7.8 8.2 8.2 7.3 7.3 158 221 221 212 212 TNRCC 191 191 TNRCC 212 212 TNRCC 224 224 221 221 207 207 194 166 225 214 214 201 201 158 158 213 213 173 173 158 277 277 211 211 490 629 629 697 697 12100302005 284 284 1047 1047 496 12100302005 264 264 615 615 425 12100301010 240 240 286 286 313 313 296 264 313 284 284 302 302 169 169 290 290 178 178 843 843 873 873 901 901 592 552 637 844 844 719 719 435 435 883 883 528 528 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 29*21'40" 98*34'59" < 0.1 < 0.1 29'21'42" 98'34-58" < 0.1 < 0.1 29'14'IS" 98'21'43" < 0.1 « < 0.1 < 0.1 0.1 0.1 0.1 < 0.1 < 0.2 < 0.1 « 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 1.2 0.4 0.4 1.6 1.6 Bexw TX 3.4 3.4 3.6 Bexar 7X 0.7 0.7 2.4 flexar TX 0.1 0.1 0.7 0.7 0.3 0.3 0.1 0.5 0.5 0.2 0.2 0.6 0.6 0.2 0.2 0.4 0.4 1.2 1.2 W=Water C= Ceriodtphnit dubit S= Cyprinodon vtriegttut S=Sedlment P= Pf/mptietos pronetes D= Daphnit putox (*) Significant Difference O N° Significant Difference Crustaceans - mortality. Fish • mortality, teratogenicity, or abnormal swimming behaviour. YPF - average number of young per female - applicable only to Canbdap/m/a dubi*. Weight - applicable only to fish larval survival and growth test 37 ------- Station Humber Waterbody Description Agency EPA Reach Latitude Longitude County State Sample Test Date of Collection Type Species 1911.0352 31-Jul-90 31-Jul-90 1911.0359 31-Jul-90 31-Jul-90 1911.0390 31-Jul-90 31-Jul-90 2202.0600 13-Feb-90 13-Feb-90 30-May-90 30-May-90 21-Jan-91 01-Apr-91 01-Apf-91 10-Jun-91 10-Jun-91 OI-Dec-93 01-Dec-93 01-Dec-93 01-Dec-93 2302.0025 21-Oct-92 21-Oct-92 25-Jan-93 25-Jan-93 20-Apr-93 20-Apr-93 21-Jul-93 Sample Types Test Percent Efwcted Control Percent significant Test Effected Effects YPF Test Control Significant Test Total Total Unionized Control Significant Weight Weight Dlf In Duration pH Alkalinity Hardness Conductivity Chlorine Ammonla-N Ainmonla-N YPF DtflnYPF (mg) (mg) Weight (Days) (S.U.) (mgfl.) (mgfl.) (umnos/cm) (mgfl.) (mgfl.) (mgfl) San Antonio River Above Confluence With Medina River W C W P 10 3 ° P 3 O 18.8 19.5 O 7 7 8.2 8.2 San Antonio River Just Below Off/to Dam W C W P 10 7 0 Q 3 O 17.8 19.5 o 7 7 8.2 8.2 San Antonio River at River Bond Park, Near Southern Road W C W P 0 3 0 Q 3 O 19.4 19.5 o 7 7 8.1 8.1 Arroyo Colorado Mala F kxdway In Uano Grande at FM 1015 South of Weslaco W C W P W C W P W C W C W P W C W P W C W P S C S P 100 3 10 10 10 0 10 80 7 20 3 0 7 0 ® 3 Q 20 Q 0 Q 0 Q 10 Q 7 Q 0 © 3 Q 10 Q 3 Q o Q 3 O 17.6 16.8 6.7 2.5 12.8 18.2 162 15.7 17.8 14 16.6 19.7 0 (§) (§) o 2 7 7 7 7 7 7 7 7 7 7 7 7 7.7 7.7 8.1 8.1 7.7 8.2 8.2 7.6 7.6 7.8 7.8 7.9 7.9 Rto Grande 0.3km Downstream from El Jardln Pumping Plant. 11.2km Downstream from Brownsvllle/Matamoros International Bridge ( W C W P W C W P S C S P S C Test Species 10 3 0 7 0 7 10 10 Q 3 Q 0 Q 3 Q 0 Q 10 Q 10 O 17.1 20 22.4 20.4 16.8 19.2 19.1 18.4 o 0 o o Significant Effects 7 7 7 7 7 7 7 8.1 8.1 8.0 8.0 7.3 7.3 7.5 TNRCC 197 197 TNRCC 203 203 TNRCC 176 176 TNRCC 222 222 232 232 243 12100301011 29'14'OS- 98*24'30* Bexar 280 857 0.1 0.4 280 857 0.1 0.4 12100301011 29*16W 98'25'40* Bexar 276 812 0.1 0.4 276 812 0.1 0.4 12100302005 29*1715* 98*3ff45* Bexar 256 712 0.1 0.4 256 712 0.1 0.4 12110208014 26*0710* 97*5739" Hidalgo 886 3730 < 0.1 1.0 886 3730 < 0.1 1.0 500 3420 < 0.1 1.0 500 3420 < 0.1 1.0 728 2550 < 0.1 0.4 TX TX TX TX 249 1000 4460 < 0.1 0.7 249 1000 4460 < 0.1 0.7 194 194 200 200 230 230 TNRCC 20 20 175 175 182 182 182 776 3470 < 0.1 1.3 776 3470 < 0.1 1.3 988 4230 < 0.1 0.7 988 4230 < 0.1 0.7 258 1101 < 0.1 13.2 258 1101 < 0.1 13.2 13090002001 25*52'35* 97*2715* Cameron 272 846 < 0.1 0.2 272 846 < 0.1 0.2 409 935 < 0.1 0.24 409 935 < 0.1 0.24 186 676 < 0.1 0.4 186 676 < 0.1 0.4 169 871 « 0.1 0.5 TX W=Water OC«riod»phnl»dubm S= Cyprinodon variaoalu* ») Significant Difference Crustacean* - mortality. S=S»dlmerrt p= flhwpnato pro/nato 0= Dep/ime pufex O f* Sflrafieant Difference Fish - mortality, teratogenkaty, or abnormal swimming behaviour. YPF-average number of young per female-applicable only to CantodapAma dubi*. Weight - applicable only to fish larval survival and growth test 38 ------- Station Number Watertody Description Agency EPA Reach Latitude Longitude County State Date of Collection Sample Type Test Species Test Percent Entcted Control Test Control Significant Test Percent Significant Test Control Significant Weight Weight Dtfln Duration Effected Effects YPF YPF Dtfln YPF (mg) (mg) Weight (Days) PH (S.U.) Alkalinity (mgfl.) Hardness Conductivity (mgfl.) (umhosrcm) Total Total Unionized Chlorine Ammonla-N Ammonla-N (mgfl.) (mgfl.) (mgfl) Continued.* Rio Grande 0.3km Downstream from El Jardin Pumping Plant, 11.2km Downstream from Brownsvllle/Matamoros International Bridge (US _^ r , . 21-Jul-93 21-Od-93 21-Oct-93 12-Apr-94 12-Apr-94 12-Apr-94 12-Apr-94 S W W W W S S P C P C p C p 0 0 7 0 7 0 0 3 ( 0 ( 3 ( 0 ( 3 ( 0 ( 3 ( J J 21.4 17.3 O J 20.8 18.9 O J J 20.4 16.3 O J 7 7 7 7 7 7 7 7.5 7.6 7.6 7.4 7.4 7.4 7.4 182 160 160 146 146 170 170 169 358 358 475 475 129 129 871 1638 1638 2070 2070 844 844 < 0.1 0.2 0.2 0.2 0.2 < 0.1 < 0.1 0.5 0.4 0.4 0.4 0.4 0.7 0.7 2302.0100 Rio Grande at International Bridge on US 77 In Brownsville TNRCC 13090002001 25*53'55" Cameron TX 11-Dec-90 11-Dec-90 18-Nov-91 18-Nov-91 11-Mar-92 11-Mar-92 02-Jun-92 02-Jun-92 W W W W W W W W C P C P C p C p 10 0 0 7 0 0 20 3 0 ( 3 ( 0 ( 3 ( 0 ( 10 ( 0 ( 7 ( ~) 17.8 J J 12.3 J J 18.5 J J 14.8 J 19 10.3 16.9 15.3 O 0 0 O 7 7 7 7 7 7 7 7 8.2 8.2 8.3 8.3 7.7 7.7 8.1 8.1 300 300 216 216 127 127 118 118 137 137 427 427 260 260 249 249 1133 1133 1590 1590 795 795 1070 1070 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 1.2 1.2 0.7 0.7 1.7 1.7 0.5 0.5 2302.0150 Rio Grande below Anheto Drain South of Las Mllpas, at River km 244.1 TNRCC 13090002002 26'03'47" 98<10*59> Hidalgo TX 21-Oct-92 21-Oct-92 25-Jan-93 25-Jan-93 20-Apr-93 20-Apr-93 21-Jul-93 21-Jul-93 20-Oct-93 20-Oct-93 12-Apr-94 12-Apr-94 12-Apr-94 12-Apr-94 2303.0100 19-May-93 Sample Types W W W W S S S S W W W W S S C P C P C p C p C p C p C p 0 0 0 7 0 0 0 3 0 0 0 0 0 0 o O 3 Q 0 Q 3 Q 0 Q 10 Q 10 Q 3 Q 0 Q 3 Q o Q 3 Q o Q 3 O 14.7 15.8 20.2 19.2 21.5 19.1 20.6 18.4 20.1 17.3 21.3 18.9 19.9 16.3 o o o o o o o 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7.9 7.9 7.6 7.6 7.1 7.1 7.6 7.6 7.3 7.3 8.1 8.1 7.1 7.1 Falcon Lake at International Boundary Monument XIV W Test Species C 0 0 O 21.2 18.7 o Significant Effects 7 7.8 126 126 100 100 208 208 219 219 108 108 118 118 252 252 TNRCC 119 256 992 256 992 297 750 297 750 163 501 163 501 198 629 198 629 275 1371 275 1371 283 1150 283 1150 296 751 296 751 262 1020 < 0.1 < 0.1 •« 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 0.1 0.1 < 0.1 < 0.1 0.2 0.2 26'51'52" 9 < 0.1 0.6 0.6 1.2 1.2 1.8 1.8 0.4 0.4 < 0.1 < 0.1 0.8 0.8 1.3 1.3 9'18'2r Zapata TX 0.4 W=Water C= Cariodtphnit dubit S= Cyprinodon variagahu ® Significant Difference Crustaceans • mortality. S=Sediment P= Pimephmlaa promote D» Dtphnif potox (j No Significant Difference Fish - mortality, teratogenkity, or abnormal swimming behaviour. YPF - average number of young per female - applicable only to Carxxtopnn/e dubia. Weight - applicable only to fish larval survival and growth test 39 ------- Station Number Waterbody Description Agency EPA Reach Latitude Longitude County State Sample Oat* of Collection Type Teat Species Twt Control PWC^flt PWCWIt Effected Effected Significant Teat Effects YPF Twt Control Significant Test Total Total Unionized Control Significant Weight Weight Dtfln Duration pH Alkalinity Hardnen Conductivity Chlorine Ammonla-N Ammonta-N YPF NflnYPF (ing) (mg) Weight (Days) (S.U.) (mgfl.) (mgfl.) (umhoafcm) (mg/L) (mg/L) (mg/l) Continued-. Falcon Ufa at totemafonalBounday Monument XV 19-May-93 W 17-Aug-93 W 17-Aug-93 W P C P 3 3 0 0 0 3 0 0 16-7 O 17.5 O 2304.0075 Rio Grande at Pipeline Crossing, 13.2 km Downstream from Old Laredo/Nuevo Laredo International Bridge (US81), 23nAug-89 W 23-AUQ-89 W 18-Nov-91 W 18-Nov-91 W 11-D0C-91 W H-Oeo-91 W 11-Mar-92 W 11-Mar-92 W 09-Jun-92 W 09-Jun-92 W 21-CW-92 W 21-Oct-92 W 26-Jan-93 W 26-Jan-93 W 12-Apr-93 S 12-Apr-93 S 06nAug-93 S OS-Aug-93 S 28-Oct-93 W 28-Oct-93 W 14-Apr-94 W 14-Apr-94 W 14-Apr-94 S 14-Apr-94 S C P C P C P C P C P C P C P C P C P C P C P C P 0 0 0 7 10 0 0 3 0 0 7 3 10 0 7 10 0 0 10 3 0 0 7 3 0 0 3 3 0 0 3 3 100 0 7 0 0 0 7 3 0 0 0 3 0 0 7 3 O 19.6 Q Q in (J Q 16.8 ^) O 17.6 ^) O n o Q 12.2 Q Q 22.1 rj Q 22.4 o MM Q Q 17.2 Qj Q 19-9 (J Q 19.2 o 20.1 10.3 19 16.9 15.7 15.8 19.2 19.6 16.8 19.2 16.3 O o o o o ® o 0 o o o 7 7 7 7.8 8.4 8.4 at River km 567.6 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 8.1 8.1 8.2 8.2 8.3 8.3 8.2 62 8.0 8.0 8.0 8.0 7.7 7.7 7.2 12 7.0 7.0 7.7 7.7 7.2 7.2 7.0 7.0 2304.0080 RbGrarte 9.0 Km Downstream of US 81 Bridge In Laredo 23-Aug-89 W 23-Aug-89 W C P 0 0 0 7 0 19-5 O 20.1 o 7 7 8.1 8.1 2304.0095 Rio Grande SO Yards Upstn3am from ConOuenoewUiZacata Creek 119 110 110 TNRCC 122 122 149 149 143 143 147 147 142 142 136 136 139 139 210 210 226 226 112 112 164 164 313 313 TNRCC 123 123 TNRCC 262 1020 264 1020 264 1020 13080002001 314 974 314 974 257 866 257 866 237 641 237 641 249 905 249 905 249 712 249 712 70 698 70 698 285 713 285 713 186 493 186 493 215 684 215 684 229 959 229 959 305 1310 305 1310 277 740 277 740 13080002001 310 967 310 967 13080002007 < 0.1 < 0.1 < 0.1 27-24'13" 99-29-13" « 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 0.4 0.1 0.1 Webb TX 1.1 1.1 0.5 0.5 1.2 1.2 0.4 0.4 0.5 0.5 0.5 0.5 1.44 1.44 3.6 3.6 < 0.1 10.8 < 0.1 10.8 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 27*2ff21" 99*29'44- < 0.1 < 0.1 27*29'55- 99*29'40> 0.5 0.5 0.5 0.5 8.4 8.4 Webb TX 1.2 12 Webb TX 23-Aug-89 W O 20.4 20.1 o 8.2 121 312 959 < 0.1 0.7 Sample TvBee TeitSoeclei W=Watar O Ceribdepnnfc duNa S= Cyprtnodon vanbgefua SsSedlment P= Hmapnafes promote D= OepAnfc putar Significant Effect* § Significant Difference Cmstaceen* - mortality. No SiQnincttn Diffwsoc* Fish * rnortBlity, tornoQtnicjty, or •bnonruil swimming YPF- average number of young per female, applicable only to Ceribdapnnfe dublt. Weight - applicable only to ftah huval tunlval and growth teat 40 ------- Station Number Watertody Description Agency EPA Reach Latitude Longitude County State Sample Test Date of Collection Type Species Continued... 23-Aug-89 2304.0098 23-Aug-89 23-Aug-89 2304.0100 23-Aug-89 23-Aug-89 2304.0150 04-Feb-91 04-Feb-91 03-Deo-91 03-Dec-91 02-Mar-92 02-Mar-92 30-Jun-92 03-Nov-92 03-Nov-92 03-Nov-92 03-Nov-92 08-Mar-93 08-Mar-93 08-Mar-93 08-Mar-93 25-Oct-93 25-Oct-93 2304.0225 15-Dec-92 15-Dec-92 15-Dec-92 27-Oct-93 27-Oct-93 Sample Types W=Water SsSadirlMnt Test Pwcwit Effected Control Test Control Significant Test Percent Significant Test Control Significant Weight Weight Dlf In Duration Effected Effects YPF YPF Dlf In YPF (mg) (mg) Weight (Days) Total Total Unionized pH Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-N (S.U.) (mg/L) (mg/L) (umhos/cm) (mg/L) (ma/L) (mg/l) Rk) Grande 50 Yards Upstream from Confluence with Zacata Creek "" *" •'" W P 0 7 O 7 8.2 Rto Grands 30 Meters Upstream of US 81 Bridge (Convent Ave) In Laredo W C W P 0 7 ° fi 7 O 18.4 20.1 O 7 7 8.0 8.0 Rto Grands at Laredo Water Treatment Plant. 5.1km Upstream from Old Larodo/Nuevo Laredo International Bridge (US81). at River km W C W P 0 7 ° P 7 O 18.5 20.1 O 7 7 8.3 8.3 Rto Grands 1 4 km Downstream from Eagle Pass/Pledras Negras International Bridge, near Irrigation Canal Lateral 50, at River Km 785. W C W P W C W P W C W P W C W C W P S C S P W -C W P S C S P W C W P 0 7 0 10 0 3 10 10 7 0 0 10 0 0 3 10 0 0 Q 10 Q o Q 3 Q o Q 3 Q 0 Q 10 Q 3 Q 10 Q 3 Q 0 Q 3 Q o Q 7 Q o Q 3 O 13.7 18.8 16.3 15.4 12.1 20 22.2 20.9 14.7 15 O 16.7 O 16.2 O 13.5 Q 16.3 ® 17.4 O 18.7 O 19.7 O 17.3 ® 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7.8 7.8 8.0 8.0 7.4 7.4 7.7 7.8 7.8 7.2 7.2 7.7 7.7 7.2 7.2 7.8 7.8 Rto Grands 4.5 Miles Downstream of Del Rto at Moody Ranch W C W P S C W C W P Test Species C= Coriodaphnia dubia Pa Pimoohaloa Dfomofaa 0 0 0 0 0 0 Q 3 Q 0 Q o Q 3 O 19.2 13.2 19 18.7 O 15.2 Q 17.3 O 7 7 7 7 7 7.4 7.4 7.4 7.8 7.8 Significant Effects S= Cyprinodon variogatua (W) Significant Difference Crustaceans - mortality. D* Dao/ifiM 01 jlm ( INo Significant C Nfferanee Fi*h - mortality, teratooenlcitv. or abr lormal swimminc i behaviour. 121 312 959 TNRCC 13080002007 121 312 121 312 967 967 TNRCC 13080002007 125 316 125 316 983 983 TNRCC 13080002018 90 261 90 261 153 245 153 245 147 245 147 245 140 240 124 252 124 252 211 179 211 179 139 286 139 286 251 165 251 165 118 217 118 217 rNRCC 131 285 131 285 194 215 127 229 127 229 907 907 727 727 907 907 893 582 582 582 582 650 650 405 405 1068 1068 911 911 608 1002 1002 < 0.1 0.7 27'29-Sr 99*3076- < 0.1 1.2 < 0.1 1.2 27*31'23- 99'31'26" < 0.1 0.6 < 0.1 0.6 28'39'48- 100'30-Orr < 0.5 0.5 < 0.5 0.5 < 0.1 0.5 < 0.1 0.5 < 0.1 0.2 < 0.1 0.2 < 0.1 0.4 < 0.1 0.4 < 0.1 0.4 2.5 2.5 2.5 2.5 < 0.1 0.2 < 0.1 0.2 < 0.1 3.6 < 0.1 3.6 0.1 0.5 0.1 0.5 29'17'31" 100*52'34" < 0.1 0.1 < 0.1 0.1 < 0.1 1.92 0.1 0.5 0.1 0.5 YPF - average number of young per female - applicable only to Weioht . .DDlieahle o nlvtofishh •rval sunHval and orawth test Webb TX Webb TX Maverick TX Klnney TX Coriodaphnlt dubia. 41 ------- Station Number Watertody Description Agency EPA Reach Latitude Longitude County State Dflte of Collection 2304.0250 16-Mar-93 16-Mar-93 16-Mar-93 16-Mar-93 Sample TVpt TMt Species TMt Percent Effected Control Test Control Significant Test Percent significant Test Control Significant Weight Weight OK In Duration pH Effected Effects YPF YPF DtflnYPF (mg) (ing) Weight (Days) (S.U.) Rio Grande S.4km Downstream from Del Rto/Cludad Acuna International Bridge W W S s C P C P 0 0 0 0 0 ( 7 ( 0 ( 3 C 3 22.2 19.7 O J 20.6 19.4 O 7 7 7 7 8.0 8.0 7.6 7.6 Alkalinity (mart.) Hardness Conductivity (mg/L) (umhosrcm) Total Chlorine (mg/L) Total Unionized Ammonla-N Ammonla-N (mgfl.) (mgfl) 77WRCC 13080001014 29'18'23" lOO'SS'OS' Val Verde TX 142 142 244 244 258 258 194 194 850 850 552 552 < 0.1 < 0.1 « 0.1 < 0.1 0.4 0.4 3.0 3.0 2306.0300 Rfo Grande below Rio Conchos Con/IUence, 14.4 km Downstream from Preskto/Ofnaga International Bridge, at River km 1,528.5 TNRCC 13040201001 29'32W 104*21W Presidio TX 30-Jan-92 30-Jan-92 20-May-92 20-May-92 21-Jul-92 21-Jul-92 20-Aug-92 20-Aug-92 09-Fab-93 09-Fab-93 11-Mar-93 11-Mar-93 14-Apr-93 14-Apr-93 15-Jun-93 15-Jun-93 25-Jan-94 25-Jan-94 25-Jan-94 25-Jan-94 W W W W W W W W S S W W s s W W W W s s C P C P C P C P C P C P C P C P C P C P 0 7 10 7 0 0 0 7 0 3 0 7 0 0 0 30 0 7 0 0 10 7 0 7 10 3 0 0 0 7 0 3 0 3 0 3 0 3 0 3 O ".1 {*) O 17.3 Q Q 19.6 rj Q 17.7 Qj Q 222 (J Q 20.6 {~) Q 22.6 Q Q 17.5 © Q 20 (J Q 22.3 O 16.2 16.2 18.2 15.7 19.9 18.7 19.6 19.3 21.4 21.1 O 0 O O O O O O 0 O 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 8.2 8.2 7.9 7.9 8.0 8.0 8.1 8.1 6.9 6.9 7.8 7.8 7.0 7.0 8.1 8.1 8.0 8.0 7.1 7.1 196 196 160 160 124 124 158 158 201 201 131 131 174 174 167 167 175 175 181 181 237 237 237 237 196 196 244 244 157 157 278 278 186 186 282 282 400 400 175 175 798 798 826 826 1022 1022 1084 1084 480 480 786 786 710 710 1103 1103 1978 1978 787 787 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 « 0.1 < 0.1 < 0.1 < 0.1 « 0.1 0.1 0.1 < 0.1 < 0.1 0.5 0.5 O.S 0.5 0.5 0.5 0.4 0.4 1.8 1.8 0.2 0.2 1.0 1.0 0.4 0.4 0.5 0.5 1.9 1.9 2308.0050 Rfo Grande at Zaragosa International Bridge In El Paso, at River km 1,992.8 TNRCC 13040100016 31'4ffOO" El Paso TX 14-Apr-92 14-Apr-92 01-Sep-92 01-Sep-92 23-Feb-93 23-Feb-93 W W W W W W C P C P C P 10 10 40 13 0 0 0 3 0 3 0 0 Q 10.8 Q ® 12.8 Q Q 20.1 O 18.2 15.3 20.8 ® O O 7 7 7 7 7 7 8.0 8.0 7.7 7.7 7.4 7.4 184 184 164 164 181 181 253 253 256 256 298 298 184 184 870 870 1066 1066 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 < 0.1 3.0 3.0 0.9 0.9 1.9 1.9 Sample Types Test Species Significant Effects W=Water C= Cwfadapnnfc duUa S= Cyprinodon varfepahn @ Significant Difference Crustaceans - mortality. S=Sediment P= HmepAafea promote D= Dtphntm pufex Q No Significant Difference Fish • mortality, teratogenicity, or abnormal swimming behaviour. YPF-average number of young per female-applicable only to Cenbdep/infc due/a. Weight - applicable only to fish larval survival and growth test 42 ------- Station Number Watertoody Description Agency EPA Reach Latitude Longitude County State Sample Test Date of Collection Type Species Continued... 22-Jun-93 22-Jun-93 22-Jun-93 22-Jun-93 20-Jul-93 20-Jul-93 20-Jul-93 20-Jul-93 28-Sep-93 28-Sep-93 28-Sep-93 28-Sep-93 2314.0100 22-Jun-93 22-Jun-93 28-Sep-93 28-Sep-93 28-Sep-93 28-Sep-93 2434.0100 30-Sep-92 10-Dec-92 11-Mar-93 18-Jun-93 09-Sep-93 07-Dec-93 2484.0200 23-Feb-93 23-Feb-93 23-Aug-93 23-Aug-93 Test Control Test Control Significant Test Percent Percent Significant Test Control Significant Weight Weight Drfln Duration pH Effected Effected Effects YPF YPF DrflnYPF (mg) (mg) Weight (Days) (S.U.) Rto Grande at Zaragosa International Bridge In El Paso, at River km 1,992.8 WC 0 0 Q 21.9 18.6 O W S S W W S S W W S S P C P C P C P C P C P 7 3 (J 0 0 Q 20.3 20 7 0 Q 0 0 Q 21 20.2 3 3 Q 0 10 Q 19.9 18.4 3 3 Q 0 0 Q 19.1 16.3 7 0 Q 0 0 Q 17.7 19.9 0 3 O O O O O ® 7 7 7 7 7 7 7 7 7 7 7 7 7.7 7.7 8.0 8.0 7.7 7.7 7.4 7.4 7.8 7.8 7.7 7.7 Rio Grande at Courchesne Bridge (Hwy 273) In El Paso, 2. 7 km Upstream from American Dam, at River km 2, 020. 8 W W W W S S C P C P C P 0 0 Q 21.7 18.6 03Q 0 0 Q 1" 16.3 7 0 Q 0 0 Q 15.8 18.2 3 3 O O O O 7 7 7 7 7 7 8.4 8.4 8.3 8.3 7.6 7.6 Christmas Bay Midway Between Christmas Point and Rattlesnake Point W W W W W S S S S S S S 3 3 Q 7 0 Q 0 3 Q 0 7 Q 7 3 Q 0 3 O 8 9 9 9 9 9 7.7 7.7 7.9 7.6 8.2 7.4 Corpus Christl Inner Harbor near Navigation Boulevard Draw Bridge W S W S S S S S 0 3 Q 0 3 Q 17 7 Q 10 7 O Sample Types Test Species W=Water S=Sediment C= Canbdapnn/a dubia P= nVnopnafos premeto S= Cyprinodon vtriegttus (•) Significant Difference D=0epnmapufex (_) No Significant Difference Significant Effects Crustaceans - mortality. 9 9 9 9 7.7 7.6 7.9 8.3 Fish - mortality, teratogenicity, or abnormal swimming behaviour. Alkalinity Hardness (mg/L) (mgfl.) 171 171 173 173 173 173 124 124 182 182 169 169 155 155 242 242 225 225 112 112 267 267 146 146 Total Total Unionized Conductivity Chlorine Ammonla-N Ammonla-N (umhos/cm) (mgn.) (mgfl.) (mg/l) 587 587 967 967 1027 1027 471 471 1230 1230 575 575 TORCC 182 182 194 194 228 228 258 258 296 296 146 146 993 993 1251 1251 954 954 TNRCC 12040205005 128 103 103 95 116 105 2000 2016 2996 2180 32800 34100 21200 28200 47700 25800 TNRCC 106 127 99 163 YPF- 2016 2016 2000 2000 37200 22400 41700 29600 0.2 0.1 0.2 0.1 < 0.1 1.6 < 0.1 1.6 0.1 1.8 0.1 1.8 < 0.1 3.6 < 0.1 3.6 0.2 0.4 0.2 0.4 0.1 1.4 0.1 1.4 31'48'10" 106'32'25" < 0.1 1.2 < 0.1 1.2 < 0.1 0.1 < 0.1 0.1 < 0.1 9.6 < 0.1 9.6 29'03'30- 95MriO- < 0.1 0.6 < 0.1 0.5 < 0.1 0.1 < 0.1 3.0 < 0.1 4.8 < 0.1 0.2 27*49'10" 97'2700" < 0.1 0.4 < 0.1 3.6 0.2 3.6 0.1 15.6 average number of young per female - applicable only to El Paso TX Brazoria TX < 0.1 AAieces TX < 0.1 < 0.1 Coriodaphnia dubia. Weight - applicable only to fish larval survival and growth test 43 ------- |