EPA/906/R-94/910
                 United Slates          Region 6               July 1994
                 Environmental Protection     Environmental Services Division
                 Agency             Dallas, Texas 75202-2733
                 Region 6 State/EPA
                    *^
                 Ambient Toxicity Monitoring  Program

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                      U.S. EPA Region 6
                             State/EPA
         Ambient Toxicity Monitoring Program
July 1994

Charlie Howell1, Terry Hollister2, & Abel Euresti2

Environmental Analysis Section
Surveillance Branch
Environmental Services Division
U.S. Environmental Protection Agency, Region 6
1445 Ross Avenue
Dallas. Texas  75202-2733

Inorganics Section
Houston Branch
Environmental Services Division
U.S. Environmental Protection Agency, Region 6
10625 Fallstone Road
Houston, Texas 77099

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                                       Executive Summary

Ambient toxicity testing, as a water quality assessment tool, complements chemical monitoring by allowing
observation of the effects of multiple contaminants or contaminant sources on test organisms. Although
biosurveys provide similar information, aquatic community impairment resulting from conventional
pollutants or habitat degradation may mask the effects of toxicants. Laboratory toxicity testing also offers
the ability to observe reproductive or teratogenic effects of contaminants that may not be apparent from a
community-level biosurvey.

The Region 6 State/EPA Ambient Toxicity Monitoring Program was established in 1990 to encourage States
to integrate ambient toxicity testing into water quality assessments, to encourage States to acquire testing
capabilities, to assess potential toxicity in Region 6 waterbodies, and to evaluate the effectiveness of
implemented toxicity control measures. Regional staff encourage State and local agencies to participate in
the program by collecting samples at routine monitoring sites. Samples are analyzed in the Region 6
Houston Laboratory using standard tests. Lab personnel forward results to the Region 6 Environmental
Services Division and the collecting agency. Currently, over 1,050 tests have been conducted on samples
collected from 144 stations.

Regional staff recommend quarterly collection of water samples and biannual collection of sediment for a
minimum of one year per station.  Continued monitoring is recommended for waterbodies that exhibit toxic
effects. The database also includes the results of one-time sample collections made during special
studies. This report summarizes data collected to date and informs potential data users of the existence
and capabilities of the program database. The report will serve to document the current database design
and solicit ideas to maximize its utility.  The validation of toxicity test results using  in situ toxicity testing and
the results of biosurveys for selected waterbodies is also discussed.

About one-third of the stations sampled on  multiple dates have exhibited recurrent toxic effects to at least
one test species (Appendix B). This percentage is high, because investigators have targeted waterbodies
with known or suspected water quality problems. Waterbodies that have exhibited recurrent toxicity are
candidates for more intensive assessments, aimed at confirming the occurrence  of toxic conditions or
aquatic life use impairment, and determination of sources or causes of toxicity.

The ambient toxicity monitoring program has provided a useful assessment tool for the identification of
waterbodies with the potential for aquatic life impairment. Response from participating agencies suggests
that interest in the program remains high. Requests for increased resources for testing and additional
testing capabilities (bulk sediment & marine tests) have been received. Opportunities for aquiring these
capabilities should be explored. Also, the Regional Environmental Services Division should assume an
active role in assisting other agencies with special studies required to determine or document the sources of
toxicity for waterbodies that have exhibited toxic effects.
Additional copies or updates of this report may be obtained by contacting:

Environmental Analysis Section (6E-SA)
Environmental Protection Agency Region 6
1445 Ross Avenue
Dallas, Texas 75202-2733
(214)665-8354
howell.charlie@epamail.epa.gov

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                                     Acknowledgments

Willie Lane, Region 6 Environmental Services Division (ESD) Houston Branch, developed the original
project plan and sampling protocols, coordinated site selection and sample collection among the agencies,
and developed the original database. Philip Crocker, Region 6 Water Management Division, deserves
recognition for the original monitoring program concept and initial development.  Paul Koska, Region 6
ESD, helped to acquire station location data.

Terry Hollister and Abel Euresti, EPA Houston Lab, deserve special recognition for their contributions to the
program. While conducting ambient tests, they have exposed over 5,000 Ceriodaphnia dubia and 14,000
fathead minnows, and hatched over 150,000 young Ceriodaphnia dubia.  Also, they have conducted all of
the required statistical tests for data interpretation and associated chemical analyses.

The following State personnel have participated either through program coordination or sample collection:

Arkansas Department of Pollution Control and Ecology
John Giese

Indian Nations Council of Governments
Richard Smith

Louisiana Department of Environmental Quality
Emelise Cormier, Sherri Courtney, John Kern

New Mexico Environment Department
David Tague, Larry Smolka

Oklahoma Department of Environmental Quality
Sylvia Rrtzsky, Jay Wright, Jimmie Pigg

Oklahoma Water Resources Board
Shon Simpson, Phil Moerschel, Main Hutcheson

Oklahoma Conservation Commission
Kendra Eddleman, Dan Butler

Texas Natural Resources Conservation Commission
Steve Twidwell, Christine Kolbe, Patrick Roques, Jeff Bertl, Wilson Snyder, Charlie Webster, Carlos
Rubenstein, Sergio Mendez, Greg Larson, Bill Harrison, Suzanne Meyers, Jim Thompson, Linda Broach,
Mark Luedke, Augustine de la Cruz, Jack Davis, Christine O'Keefe, Brenton Wade, Art Crow

Texas Parks and Wildlife Department
Dave Buzan, Tim Jurgensen

We also thank Philip Crocker, Phillip Jennings, Wren Stenger, and Jack Ferguson, Region 6, for review and
comment on the draft report.  In addition, Stephen Twidwell and Cassy  Shaukut, Texas Natural Resources
Conservation Commission, conducted an extensive review of the report and data collected by the State of
Texas.
                                             IV

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                                      Table of Contents





Executive Summary	 iii



Acknowledgments	 iv



Introduction	 1



Methods	 1



Data Compilation	2



Results and Discussion	 3



Potential for Development of Environmental Indicators 	 5



Conclusions & Recommendations 	 5



Literature Cited 	 7



Appendix A. Database Field Definitions 	 9



Appendix B. Waterbodies That Have Exhibited Toxic Effects 	 12



Appendix C. Ambient Toxic'rty Test Results and Laboratory Chemical Data	 15





                                            Table



1.  Species & Test Methods Used in the Program 	 2





                                           Figures



1.  Database Structure 	 3



2.  Samples in the Database 	 3



3.  Species Used for Toxicity Tests	 3



4.  Relative Sensitivity of Ceriodaphnia dubia and Pimephales promelas 	4



5.  Linnville Bayou at CR 324 - Ceriodaphnia dubia Mean No. of Young per Female 	 6



6.  Linnville Bayou at CR 324 - Pimephales promelas Test Effects	 6

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                                          Introduction

Environmental Protection Agency (EPA) Region 6 has conducted an effluent toxicity testing program for
over thirteen years. The Regional laboratory conducts standard tests by evaluating the response of
organisms exposed to municipal and industrial effluents. Besides detection of effluent toxicity, results have
been used to evaluate the sensitivity of test methods, and the relative toxicity of Standard Industrial
Categories (Crocker, ej aj. 1989). Although useful from a regulatory standpoint, effluent testing is of limited
value for water quality assessment, because of the inability to evaluate potential synergistic, antagonistic, or
additive effects of multiple contaminant sources. Investigators have documented the ability to predict
aquatic community response based on results of effluent testing in conjunction with ambient toxicity testing
(Bastian ej aj., 1990; Birge ej aj., 1989; and Mount ej a]., 1985a-b and 1986a-d).

Ambient toxicity testing, as a water quality assessment tool, complements chemical monitoring with the
direct assessment of the compound physiological effects of multiple contaminants or contaminant sources
on test species. Although biosurveys provide similar information, aquatic community impairment resulting
from conventional pollutants or habitat limitations may mask potential effects of toxicants. Laboratory
toxicity testing also offers the ability to observe reproductive or teratogenic effects of contaminants that may
not be apparent from a community-level biosurvey.

In 1990, the Region 6 State/EPA Ambient Toxicity Testing Program was initiated to establish a system for
the assessment of ambient toxicity. The program was developed to encourage State agencies to integrate
ambient toxicity testing into water quality assessments, to encourage States to acquire  testing capabilities, to
assess potential toxicity in  Region 6 waterbodies, and to evaluate the effectiveness of implemented toxicity
control measures. Regional staff encourage State and local agencies to participate in  the program by
collecting samples at monitoring sites. Samples are tested by the Region 6 Houston  Laboratory.  Lab
personnel forward results to the Region 6 Environmental Services Division and the collecting agency.

Region 6 States have participated in the program to varying degrees.  Oklahoma and Texas agencies have
collected water samples quarterly from selected sites. The New Mexico Environment Department has
collected samples during intensive surveys. The Arkansas Department of Pollution Control and Ecology
and the Louisiana Department of Environmental Quality have collected samples for a special study on the
Mississippi River. Currently, over 1,040 tests have been conducted on samples from about 144 stations.
Data from 180 tests conducted during a special study on the Rio Grande are unavailable for public review,
pending completion of the final study report. These results were included in summary statistics, but are not
included in data tables.

This report will summarize data collected to date and inform potential data users of the existence and
capabilities of the database. Also, the report investigates potential use of the data for environmental
indicators, and encourages continued participation in the program.  The report will serve to document the
current database design and solicit ideas to maximize its utility.


                                            Methods

Regional staff recommend quarterly collection of ambient water and biannual collection of sediment for a
minimum of one year to increase the probability that intermittent toxicity will be detected.  Continued
monitoring is recommended for waterbodies that have exhibited toxic effects. The database also includes
the results of one-time collections made during intensive surveys or special studies.

Grab samples of ambient water are collected on a single day, in sufficient volume (two gallons) to provide
for renewal of test water throughout the test period. This approach is inconsistent with  guidance (Weber ej
aj., 1989), developed primarily for regulatory application, which suggests daily sampling or a minimum of
three samples collected every other day. The decision to collect a single sample for testing is based on
consideration of personnel and travel costs for renewal sample collection. Samples are generally collected
during other ambient monitoring, and require no additional travel. This approach has proven effective for
the primary objective of this program, i.e., screening for potential ambient toxicity.

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Water samples are collected from the main flow of the stream in a clean polyethylene container(s) rinsed
with ambient water before filling. Sediment samples are composited from a minimum of three subsamples
of the upper layer of recently deposited, unconsolidated sediment. Subsamples are composited and mixed
before being transferred to a clean glass or polyethylene containers).  Collecting equipment is thoroughly
cleaned between sites and rinsed with ambient water before use. Upon collection, water and sediment
samples are placed in ice for shipment to the laboratory.

In the laboratory, sediment elutriates are prepared by combining a subsample from the homogenized
sediment sample with appropriate culture water. The sediment and water are combined in a sediment-to-
water ratio of 1:4 by volumetric displacement. After combining, the mixture  is tumbled end-over-end for
approximately 24 hours at 3 - 4 °C. After settling, the elutriate is siphoned off and filtered through a 1.5
micron glass fiber filter.

Standard laboratory tests and statistical data analyses are conducted according to Short-term Methods for
Estimating the Chronic Toxic'rtv of Effluents and Receiving Waters to Freshwater Organisms (Weber, et a].,
1989), Short-Term Methods for Estimating the Chronic Toxicitv of Effluents and Receiving Waters to
Marine and Estuarine Organisms (Weber, et a]., 1988), and Methods for Measuring the Acute Toxic'rty of
Effluents to Freshwater and Marine Organisms (U.S. EPA, 1991). Test species and methods are described
in Table 1.
   Table 1.  Species & Test Methods Used In the Program

   Ceriodaphnia dubia seven-day survival and reproduction test; short-term chronic test observing
          mortality rate and the number of young produced per female.

   Daphnia pulex 48-hour survival test; acute test observing mortality rate.

   Pimephales promelas (Fathead Minnow) seven-day embryo-larval survival and teratogenicity test;
          short-term chronic test observing mortality rate, teratogenic effects, and abnormal
          swimming behavior.

   Pimephales promelas seven-day larval survival and growth test; short-term chronic test observing
          mortality rate and dry weight at test termination.

   Cyprinodon variegatus (Sheepshead Minnow) nine-day embryo-larval survival and teratogenicity
          test; short-term chronic test observing mortality rate, teratogenic effects, and abnormal
          swimming behavior (used for estuarine or saline waters).
                                       Data Compilation

Regional staff enter and maintain test data in a Microsoft Access for Windows™ relational database. Three
data tables contain (1) station location details, (2) toxicity test conditions and results, and (3) chemical test
results (Figure 1 & Appendix A). State station numbers and sample identification numbers are key fields
that link the tables. The software forces maintenance of referential integrity between the tables to preclude
test data entry where supporting data is nonexistent.  Data entry forms contain validation checks that
prevent entry of erroneous numeric values. The Windows™ environment provides remarkable advantages
for data entry with the ability to copy and later paste key field data to and from memory, eliminating
repetitive data entry.

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The data tables may be exported for use with other
relational database software, after reestablishing
links.  Also, some or all of the data fields may be
exported in a single table for use with spreadsheet
programs.

A backup copy of the database is made after each
data entry session. Annually, the database will be
exported to labeled diskettes in an ASCII format and
maintained by the Environmental Analysis Section.
Hardcopies of test results will also be stored in the
Houston laboratory.
  Figure 1. Database Structure
                                     Results and Discussion

About one-third of the stations sampled on multiple dates have exhibited recurrent toxic effects to at least
one test species (Appendix B). This percentage is high, because investigators have targeted waterbodies
with known or suspected water quality problems. Waterbodies that have exhibited recurrent toxJcity are
candidates for more intensive assessments, aimed at confirming the occurrence of toxic conditions or
aquatic life use impairment. Priority for investigation should be based on the frequency and degree of
observed toxicity as well as other important factors, such as waterbody designated uses, actual uses,
economic importance, endangered species, and known or suspected causes of impairment.

Initially, Oklahoma agencies were the only major source of sediment samples, as other agencies opted to
collect only water samples. Therefore, sediment data in the database is limited. With regard to test
species, Ceriodaphnia dubia and Pimephales promelas (Fathead Minnow) have been used routinely. The
Daphnia pulex acute test was in use when the program was initiated. Cyprinodon variegatus (Sheepshead
Minnow) has been used for some saline or estuarine waters. Figures 2. & 3. show the relative breakdown
of approximately 1,040 tests for sample types and test species.
 Figure 2. Samples in the Database (n=1047)

         Water
Figure 3. Species Used for Testing

  Coriodaphnle
    <**ta^-
                          Sadlment
  Cyprinodon
    varieyatus
Pimephales
 promelas
The database offers an opportunity to further examine the relative sensitivity of selected toxicity test
methods.  For Ceriodaphnia dubia and Pimephales promelas, 340 pairs of side-by-side tests have been
conducted on ambient water samples. Ninety-seven pairs of side-by-side tests were conducted on
sediment samples using these species. Twenty-eight (28%) percent of the water samples, and thirty (30%)
percent of the sediment elutriates, caused significant toxic effects to at least one test species. Graphs in
Figure 4. are based on the seventy-six water samples and nineteen sediment samples that caused toxic
effects to either one of the two species. The results strongly suggest that Ceriodaphnia dubia is a more
sensitive test species, although this comparison may be biased by specific causes of toxicity in the assessed
waterbodies. The results of Pimephales promelas  tests may be more important as an indicator of potential
causes of toxicity, rather than as an indicator of potential toxic effects, since this species indicated toxicity
only six times when Ceriodaphnia dubia did not. Similar comparisons using Daphnia pulex or Cyprinodon
variegatus were not made, because  of the limited amount of data for these species.

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Figure 4.  Relative Sensitivity of Ceriodaphnia dubia and Pimephales promelas - Results from Side-by-Side
          Testing of Samples or Elutriates that Caused Toxicity to Either Species

                                Species Indicating Significant Toxicity

             Water Samples (n=96)                             Sediment Samples (n=27)
                   Both Species    ^^H Pimephales promelaa  jggfr Ceriodaphnia dubia  (Most Sensitive)
Although data for side-by-side sediment elutriate and water testing is limited, results confirm the importance
of collecting both samples. While sediment samples are more difficult to collect, sediment elutriates
yielded positive toxicity results more frequently than water samples. These findings are consistent with
observations made by Bastian et aj. (1989).

The toxicity screening procedures, as described, have important limitations. The collection of single grab
samples allows the possibility that intermittent toxicity may not be detected. The sediment elutriation
procedure will extract only those contaminants that are water-soluble or loosely bound to the sediment
(Nriagu and Lakshminarayana, 1989). The fact that any given station has not tested positively, does not
rule out the possibility that water quality or sediment quality problems exist.

During an assessment of the Guadalupe River near Victoria, Texas, Trebatoski (1991) used both laboratory
and in situ toxicity tests. The study was designed to investigate the potential impact of a domestic
wastewater treatment facility on the  river and to examine the  utility of ambient toxicity testing. Caged
fathead minnows were exposed to the effluent and the river,  at an upstream site and two downstream sites,
for a 48-hour period. Both the in situ and  laboratory tests demonstrated effluent toxicity. The test results
differed in that acute effects were observed in the field (100% mortality after six hours), whereas, chronic
effects were observed in the laboratory (55% mortality after eight days). Results of in situ and laboratory
tests were consistent regarding instream conditions, as neither test demonstrated significant toxicity at
upstream nor downstream sites.

It should be noted that the Pimephales promelas embryo-larval survival and teratogenicity test routinely
used in the ambient toxicity monitoring program differs from the larval survival and growth test required of
National Pollutant Discharge Elimination System (NPDES) permittees. The embryo-larval test was
selected as a more cost-effective initial screening test.  When a NPDES permittee is suspected as a source
of toxicity, the larval survival and growth test should be conducted. Also, investigators should make return
trips to the srte(s) to collect samples for renewal of test water. The ambient toxicity monitoring program
sampling protocols will be revised in the future to include these recommendations.

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                       Potential for Development of Environmental Indicators

 Ambient water and sediment quality tests may serve as environmental indicators if comparisons of results
 are made over time for given stations. Because additions and deletions of stations may occur, program
 data should not be used collectively as an environmental quality indicator. For example, an agency may
 opt to temporarily discontinue monitoring of waterbodies that frequently exhibit toxicity while corrective
 actions are implemented. This may result in the appearance of improving conditions, if alternate stations do
 not exhibit toxicity.  Conversely, the selection of several new stations that continually exhibit toxicity may
 produce the appearance of deteriorating conditions.  Variability in meteorological conditions may also
 cause temporary declines or increases in the occurrence of ambient toxicity resulting from nonpoint
 sources. The data may be useful for regional environmental indicators, over a period of many years, using
 a consistently monitored subset of stations.

 On a waterbody or station-specific basis, data will be useful as an environmental indicator in shorter terms.
 For example, the installation of dechlorination or nitrification facilities at a specific treatment facility, or the
 implementation of best management practices to control nonpoint contaminant sources may result in an
 immediate reduction or elimination of the occurrence of positive toxicity tests.

 Kolbe (1994) presents an example of how ambient toxicity data are applied as an environmental indicator
 for a specific waterbody. The Phillips 66 Refinery and Petrochemical Complex located in Brazoria County,
. Texas, discharges treated wastewaters to LJnnville Bayou.  The discharge contributes almost all instream
 flow during dry weather.  Phillips 66, the only permitted point source on the bayou, discharges an average
 of four-million gallons per day.  Previous studies associated with this discharge have documented impacts
 such as oily sheens, elevated pH, low dissolved oxygen, and high ammonia and sulfide concentrations.
 Severe impacts to fish communities of LJnnville Bayou have been observed during several studies. During
 the most recent survey in August 1992, investigators collected nine fish species at an upstream site;
 however, no fish could be collected 0.4 miles downstream from the discharge (mosquitofish, Gambusia
 affinis, were observed in the shallows at the downstream site).  Only five fish species were collected nine
 miles downstream, and mosquitofish comprised 97% of the individuals in this sample. Ambient toxicity
 testing allowed investigators to identify toxicity as a probable contributing factor to observed fish community
 impairment. Effluent toxicity at this facility was attributed to chlorides, ammonia, and one or more organic
 toxicants.

 Phillips 66 has completed several treatment plant upgrades in attempts to achieve compliance with permit
 conditions. The most significant improvements resulted from the identification and removal of ammonia
 sources in September 1992 and the startup of activated sludge and powdered activated carbon treament
 systems in April 1993. Recent ambient toxicity test results suggest a reduction in the occurrence of ambient
 toxicity that appears to coincide with the removal of ammonia sources (Figures 5.  and 6.)  Historically, water
 samples collected from LJnnville Bayou at Hasema Road (Station No. 1300.0726), located immediately
 downstream from the discharge, caused toxic effects to both Ceriodaphnia dubia and Pimephales
 promelas. Recent tests have shown no significant effects to test organisms exposed to ambient water
 collected from this site. Follow-up studies are being conducted to monitor the compliance status of the
 facility and future trends in the condition of aquatic communities of LJnnville Bayou.


                                Conclusions & Recommendations

 The ambient toxicity monitoring program has proven useful for identifying waterbodies with the potential for
 aquatic life impairment. Although data for side-by-side laboratory toxicity tests and in situ testing is limited,
 results indicate that toxicity testing is a reliable indicator of instream conditions where habitat quality or
 conventional pollutants are not limiting. About one-third of the stations sampled on multiple dates have
 exhibited recurrent toxic effects to at least one test species.  The frequency of observed toxicity is high,
 because investigators have generally targeted waterbodies with known or suspected water quality

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         Fig. 5 LJhnvilto Bayou at Hasema Rd (CR 324)
       Ceriodaphnladubla Moan No. of Young par Femato
   10
 o
 §
                     Control
                            Sample
           **
                 **
                         I**
                                                       100
                                                       w
                                                       20
  *W 7JW 1(»0 «M «M 7*i 10*11M1 .
                      Data
• Significant affect (PXI.85)
** No young produced ki uinple due to mortaMy-
                                7/K M2 121*2 M* «W 12W
                                                         Fig. 6 LlnnvHe Bayou at Haaema Rd (CR 324)
                                                             Pimephatos pmmdas Test Effects
                                                                              *
                                                                                               Control
                                                                                               Sample
                                                                                    Mm MM
                                                          4NO 4m 7/01 10m 12«1 3192 7H2 6102. 1202 3*3 OK3 12/93
                                                         •StaneTcant effect (P>0.85)
                                                       Taet eftecto Include mortally, taratoganldty, or abnormal iwknmtag behavior.
problems. Waterbodies that have exhibited recurrent toxicity are candidates for more intensive
assessments, aimed at confirming the occurrence of toxic conditions or aquatic life use impairment, and
determination of sources or causes of toxicity. Effluent testing may be conducted where point sources are
suspected as the primary cause of instream toxicity. Toxicity identification evaluations (TIEs), chemical
analyses, and/or biological assessments may be useful for documenting the causes and instream effects of
observed toxicity.

States should continue to monitor those waterbodies exhibiting toxicity until successful corrective actions are
completed.  It may be prudent to temporarily discontinue monitoring at some stations to allow other
waterbodies to be screened. The Regional Environmental Services Division should assume an active role
in assisting other agencies with special studies required to determine or document the sources of toxicity for
waterbodies that have exhibited toxic effects.  For some waterbodies, such studies have been conducted or
are currently underway.  The information from these studies should be compiled for inclusion in the
database.

Response from participating agencies suggests that interest in the program remains high.  Requests for
increased resources for testing and additional testing capabilities (bulk sediment & marine tests) have been
received. Opportunities for acquiring these capabilities should be explored.

The utility of the Pimephales promotes chronic test should be reviewed in future data analyses and reports.
Data suggest that the test generally lacks the sensitivity to detect toxic  conditions that are not demonstrated
by the Ceriodaphnia dubia test. The results of Pimephales promelas toxicity tests may be more important
as an indicator of potential causes of toxicity, rather than as an indicator of potential toxic effects.

Many other data sources exist which could augment the database. An active program of data acquisition is
recommended. This will facilitate use of the data and prevent potential data loss. Efforts should also be
directed toward database modifications necessary to make the data more accessible to potential users.
                                                 6

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                                          References

Bastian, M., W. Lane, P. Koska, C. Young, T. Hollister, B. Trebatoski, L Smolka, and D. Butler. 1989. U.S.
    EPA Region 6 Ambient Toxicity Testing Demonstration Projects: Evaluation of Results. U.S.
    Environmental Protection Agency, Region 6, Dallas, Texas.

Birge, W.J., J A Black, T.M. Short, and A.G. Westerman. 1989. A Comparative Ecological and
    Toxicological Investigation of a Secondary Wastewater Treatment Plant Effluent and its Receiving
    Stream. Environmental Toxicology and Chemistry. 8:437-450.

Crocker, PA, TA Hollister, A. Euresti, and D. Neleigh. 1989. U.S. EPA Region 6 Effluent Toxicity Testing
    Program: Evaluation of Results.  U.S. Environmental Protection Agency, Region 6, Dallas, Texas.

Greene, J.C., C.L. Bartels, W.J. Warren-Hicks, B.R. Parkhurst, G.L. Under, SA Peterson, and W.E. Miller.
    1988.  Protocols for Short-Term Toxicity Screening of Hazardous Waste Sites.  Environmental
    Research Laboratory, U.S. Environmental Protection Agency, Corvallis, Oregon, EPA/600/3-88/029.

Kolbe, C.M.  1994. Effects of Petroleum Refinery Wastewater on Water Quality in LJnnville Bayou
    (Brazoria/Matagorda County, Texas). Texas Natural Resources Conservation Commission Region 12,
    Houston, Texas. Report AS-16/SR.

Mount, D.I., A.E. Steen, T.J. Norberg-King.  1985a. Validity of Effluent and Ambient Toxicity Testing for
    Predicting Biological Impact on Five Mile Creek, Birmingham, Alabama. U.S. Environmental Protection
    Agency, Environmental Research Laboratory, Duluth, Minnesota, EPA/600/8-85/015.

Mount, D.I., and T.J. Norberg-King. 1985b. Validity of Effluent and Ambient Toxicity Testing for Predicting
    Biological Impact, Scippo Creek, Circleville, Ohio. U.S. Environmental Protection Agency,
    Environmental Research Laboratory, Duluth, Minnesota, EPA/600/3-85/044.

Mount, D.I., and T.J. Norberg-King. 1986a. Validity of Effluent and Ambient Toxicity Testing for Predicting
    Biological Impact, Kanawha River, Charleston, West Virginia. U.S. Environmental Protection Agency,
    Environmental Research Laboratory, Duluth, Minnesota, EPA/600/3-86/006.

Mount, D.I., A.E. Steen, T.J. Norberg-King.  1986b. Validity of Effluent and Ambient Toxicity Testing for
    Predicting Biological Impact, Ohio River, Near Wheeling, West Virginia. U.S. Environmental Protection
    Agency, Environmental Research Laboratory, Duluth, Minnesota, EPA/600/3-85/071.

Mount, D.I., A.E. Steen, T.J. Norberg-King.  1986c. Validity  of Effluent and Ambient Toxicity Testing for
    Predicting Biological Impact, Back River, Baltimore Harbor, Maryland.  U.S. Environmental Protection
    Agency, Environmental Research Laboratory, Duluth, Minnesota, EPA/600/8-86/001.

Mount, D.I., A.E. Steen, T.J. Norberg-King.  1986d. Validity of Effluent and Ambient Toxicity Testing for
    Predicting Biological Impact, Naugatuck River, Waterbury, Connecticut. U.S. Environmental Protection
    Agency, Environmental Research Laboratory, Duluth, Minnesota, EPA/600/8-86/005.

Nriagu, J.O., and J.S.S. Lakshminarayana. 1989. Aquatic Toxicology and Water Quality Management.
    John Wiley and Sons, Inc.  New York, New York.

Trebatoski, B. 1991. Guadalupe River Toxicity Assessment. Texas Natural Resources Conservation
    Commission.  Austin, Texas. Report No. LP 91-04.

U.S. EPA.  1991. Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to
    Freshwater and Marine Organisms. U.S. Environmental Protection Agency, Office of Research and
    Development, Washington, D.C., EPA/600/4-90/027.

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                                       References, cent.

Weber, C.I., W.H. Peltier, T.J. Norberg-King, W.B. Homing II, FA Kessler, J.R. Menkedick, T.W. Neiheisel,
    PA Lewis, D.J. Klemm, Q.H. Pickering, E.L Robinson, J.M. Lazorchak, L.J. Wymer, and R.W.
    Freeburg. 1989. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving
    Waters to Freshwater Organisms. U.S. Environmental Protection Agency, Environmental Research
    Laboratory, Cincinnati, Ohio, EPA/600/4-89/001.

Weber, C.I., W.B. Horning II, D.J. Klemm, T.W. Neiheisel, PA Lewis, E.L. Robinson, J. Menkedick, and F.
    Kessler.  1988. Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving
    Waters to Marine and Estuarine Organisms.  U.S. Environmental Protection Agency, Environmental
    Research Laboratory, Cincinnati, Ohio, EPA/600/4-87/028.

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Appendix A. Database Field Definitions
Text in parentheses indicates the type of database field.

                                  Station Details - tbIStations

Agency (Text) - State or local agency responsible for sample collection, identified by abbreviation or
acronym.

County (Text) - county or parish of sampling location, identified by full name.

Description of Waterbody (Text) - narrative description of sampling station location, including major
highway crossings, references to other major landmarks, or identification of the nearest city.

EPA Reach Number (Number) - EPA River Reach identified by agency number.

Latitude (Number) - latitude of sampling point.

Longitude (Number) - longitude of sampling point.

Notes Specific to Station (Memo) - memo field used to store information specific to station, such as
suspected sources of toxic contaminants and references for special study reports.

State  (Text) - State where sample collected, identified by two-letter abbreviation.

Station Number (Text) - permanent station identifier, assigned by State or local agency. Links station
details table with toxicity test results table.


                         Toxicity Test Conditions & Results - tblResuKs

Sample ID (Text) - number or identifier assigned to water or sediment sample by laboratory. Links toxicity
test conditions table with chemical results table.

Test Date (Date) - date test initiated.

Sample Type (Text) - identifies sample matrix, as either water (W) or sediment (S).

Test Species (Text) - identifies the organism used during the test, as Daphnia pulex (D), Cen'odaphnia
dubia  (C),  Pimephales promelas (P), or Cyprinodon variegatus (S).

Test Duration (Number) - duration of test in days.

Test Type (Text) - identifies test as acute (A) or chronic (C).

Significant Mortality/Effects (Yes/No) - indicates whether or not differences in mortality or other test
effects between the control and sample were statistically significant.

Significant Difference in Young per Female (Yes/No) - indicates whether or not differences in number of
young produced per female in the control and sample were statistically significant.

Significant Difference in Weight (Yes/No) - indicates whether or not differences in dry weight of
organisms between the control and sample were statistically significant (fish tests).


                                               9

-------
Sample Effects/Mortality (Number) - percentage of test organisms affected during test. In addition to
mortality, effects may include organisms exhibiting terata, or abnormal swimming behaviour.

Control Effects/Mortality (Number) - percentage of control organisms affected during test. In addition to
mortality, effects may include organisms exhibiting terata, or abnormal swimming behaviour.

Sample Young per Female (Number) - mean number of young produced per female in sample or elutriate
during Ceriodaphnia dubia survival and reproduction test.

Control Young per Female (Number) - mean number of young per female produced in control during
Ceriodaphnia dubia survival and reproduction test.

Sample Weight (Number) - mean dry weight offish exposed to sample or elutriate, upon completion of
test.

Control Weight (Number) - mean dry weight offish exposed to control, upon completion of test.

Notes Specific to Test (Memo) - memo field used to store observations specific to test or sample
collection.


                            Chemical Results - tbIChemicalResults   .

Alkalinity (Number) - alkalinity of water sample or sediment elutriate, measured in the lab and expressed in
mg/L.

Conductivity (Number) - conductivity of water sample or sediment elutriate, measured in the lab and
expressed in umhos/cm.

Hardness (Number) - hardness of water sample or sediment elutriate, measured in the lab and expressed
in mg/L.

Laboratory (Text) - laboratory identifier. Currently, the EPA Region 6 Houston lab is the only contributor to
the database.

Nondetect Cl (Text) - denotes total chlorine concentration below detection limit.

Nondetect  Ammonia-N (Text) - denotes ammonia-N concentration below detection limit.

Nondetect Unionized Ammonia-N  (Text) - denotes unionized ammonia-N concentration below detection
limit.

pH (Number) - pH of water sample or sediment elutriate, measured in the lab and expressed in standard
pH units.

Salinity (Number) - salinity of water sample or sediment elutriate, measured in the lab and expressed in
mg/L.

Sample Collection Date (Date) - date of sample collection.

Total Ammonia-N (Number) - total ammonia-N concentration of water sample or sediment elutriate,
measured in the lab and expressed in mg/L.

Unionized Ammonia-N (Number) - unionized ammonia-N concentration of water sample or sediment


                                             10

-------
elutriate, measured in the lab and expressed in mg/L.

Total Chlorine (Number) - total chlorine concentration in water sample or sediment elutriate, measured in
the lab and expressed in mg/L.
                                              11

-------
Appendix B. Waterbodies That Have Exhibited Significant Toxic Effects in One or More Tests
               Grouped by State and Listed by Station Number
Station
State Number
Arkansas
1
2
Louisiana
S-52
S-54

New Mexico
UPR21 1.001 537

UPR21 1.001 539
Oklahoma
OB002
20B

7

OCC2

OCC5



OCC7
ODEQ10

ODEQ12



ODEQ2
ODEQ3




Abbreviated Description

Mississippi River near Elaine
Mississippi River near Arkansas City

Mississippi River across from Bell Chasse
Mississippi River across from Plaquemine


Spring Arroyo at Airport Road

Gallinas River at Grand

Bird Creek in Tulsa
Tar Creek in Miami

Tar Creek at State Line North of Miami

Gaines Creek near Lake Eufala

Pitt Creek East of Hartshome



Spring Creek East of Edmond
Acid Creek

Tributary of Chickaskia River near Blackwell



Cimarron River N. of Lone Mountain
Coal Creek near Henryetta




No of Samples Causing Toxic Effects
Sample Test Primary Secondary
Type1 Species1 Endpoint3 Endpoint4

W
W

W
W
W

W
W
W

W
W
W
W
W
S
W
S
S
W
W
W
S
W
W
W
S
S
W
S
S
S
W
W

c
c

c
c
p

c
p
c

c
c
p
p
c
c
c
c
p
c
p
c
c
p
c
p
c
p
S
c
D
p
c
p

0
0

0
0
0

1
1
1

0
3
2
2
3
1
1
2
2
2
2
2
0
1
3
1
1
1
11
2
2
4
7
6

1
1

2
1
1

0
0
1

1
0
0
0
0
2
1
0
0
0
0
1
1
0
1
0
1
0
0
0
0
0
0
0
Total No. of
Samples

6
6

10
12
12

1
1
1

3
3
2
2
3
2
6
2
2
2
2
2
2
4
3
3
2
2
11
2
2
4
8
7
'Sample - Water (W) or Sediment (S)
'Species-  Ceriodaphnia dubia (C). Pimephales promelas (P), Daphniapulex(D).  Cyprinodon variegatus (S)
•Primary Endpoints - dependent on test species, i.e., mortality for crustacean tests; mortality, teratogenicity, or abnormal swimming
behavior for fish tests.
'Secondary Endpoints - dependent on test species, i.e., significant difference in number of young per female for Ceriodaphnia dubia:
difference in weight for fish larval survival and growth tests (absence of secondary endpoint effects may be due to mortality).

                                                       12

-------
                                                                       No of Samples Gaming Toxic Effect*
Station
State Number
OOEQ4
ODEQ6
ODEQ7



ODEQ9


Texas
0214.0160
0304.0100

0601.0800
0700.0200



0805.0300
0841.0500
0902.0100

1006.0200
1007.9200
1113.0020
1200.7820

1200.7822
1200.7830


1200.7832
1200.7834
1218.0270
1300.0710

Abbreviated Description
Lake Francis at Upper End
Bitter Creek
Tributary of San Bote Creek



Tributary of Eliza Creek



Wichita River at End of Eastland Lane
Days Creek at South of Texarkana

Neches River Near Beaumont
Alligator Bayou in Port Arthur



Trinity River below Dallas
W. Fork Trinity River in Grand Prairie
Cedar Bayou Southwest of Dayton

Houston Ship Channel at Greens Bayou
Vince Bayou at Houston Ship Channel
Armand Bayou Tidal Upstream of NASA
Bryan Municipal Lake near Dam

Bryan Municipal Lake Mainbody
Finfeather Lake near Dam


Finfeather Lake Main Body
Finfeather Lake Headwater
Nolan Cr. Above Bell County WCID Outfall
Linnville Bayou Upstream from Caney Creek

Sample Test
Type1 Species'
W
W
W
W
S
S
S
W
W

W
S
S
W
S
S
W
W
W
S
W
W
W
S
S
W
S
W
S
W
W
W
W
W
W
W
c
c
p
c
c
p
c
c
p

c
p
c
c
c
p
c
p
c
c
c
p
c
S
S
c
c
c
c
c
p
c
c
c
c
p
Primary Secondary
Endpoinr Endpoint'
0
1
3
3
2
1
2
4
1

0
1
1
0
2
2
2
2
0
0
1
1
0
2
1
0
1
0
1
2
1
1
1
1
0
1
1
0
0
0
0
0
0
0
0

1
0
1
1
0
0
1
0
1
1
0
0
1
0
0
1
1
1
0
0
0
0
0
2
1
0
Total No. of
Samples
2
3
4
4
2
1
2
4
4

2
2
2
6
3
3
5
5
5
3
6
5
1
4
3
5
1
6
1
10
10
2
1
8
1
1
'Sample - Water (W) or Sediment (S)
'Species- Cortodaphniadubia (C), Pimephalespromdas(P), Daphnlapufox(D), Cyprinodon variegatus (S)
'Primary Endpoints - dependent on test species, i.e., mortality for crustacean tests; mortality, teratogenteity, or abnormal swimming
behavior for fish tests.
^Secondary Endpoints - dependent on test species, i.e., significant difference in number of young per female for Ceriodaphnia dubia:
difference in weight for fish larval survival and growth tests (absence of secondary endpoint effects may be due to mortality).

                                                          13

-------
                                                                         No of Samples Causing Toxic Effects
Station
State Number
1300.0717

1300.0726


1302.0100
1412.0140
1421.0470

1429.0800
1429.TMP1
1906.0230

1906.0240
2202.0600
2304.0075

2304.0150
2306.0300
2308.0050

Abbreviated Description
Linnville Bayou at Boone Road

Linnville Bayou at Hasema Road


San Bernard River Northeast of Boling
Colorado River in Colorado City
North Concho River in San Angelo

Town Lake at Longhom Dam
Waller Creek at 2nd Street in Austin
Leon Creek South of San Antonio

Leon Creek Below Kelly AFB
Arroyo Colorado South of Weslaco
Rio Grande Below Laredo

Rio Grande near US 277 Bridge in Eagle Pass
Rio Grande below Rio Conchos Confluence
Rio Grande at Zaragosa International Bridge

Sample Test
Type1 Species2
W
W
S
W
W
W
W
S
W
W
W
W
W
W
W
S
W
W
W
W
S
c
p
p
c
p
c
c
c
c
c
c
c
p
c
c
c
c
c
p
c
c
Primary Secondary
Endpoint3 Endpoint*
0
1
1
5
9
0
1
0
1
1
0
1
1
1
2
1
0
0
1
1
0
1
0
0
9
0
1
1
1
0
2
1
3
0
1
3
0
1
2
0
1
1
Total No. of
Samples
2
3
3
16
13
6
2
2
6
4
1
9
9
1
6
3
9
7
7
6
3
1Sample - Water (W) or Sediment (S)
'Species- Ceriodaphniadubia (C), Pimephalespromelas(P), Daphniapulex(D), Cyprinodon variegatus (S)
'Primary Endpoints - dependent on test species, i.e., mortality for crustacean tests; mortality, teratogenicity, or abnormal swimming
behavior for fish tests.
'Secondary Endpoints - dependent on test species, i.e., significant difference in number of young per female for Ceriodaphnia dubia:
difference in weight for fish larval survival and growth tests (absence of secondary endpoint effects may be due to mortality).

                                                           14

-------

-------
Appendix C.  Ambient Toxicity Test Results and Laboratory Chemical Data (Grouped by State and Agency)
Station Humber Waterbody Description
Test
Sample Test Percent
Date of Collection Type Species Effected
Control
Percent significant Test
Effected Effects YPF
Test Control Significant Test
Control Significant Weight Weight Dtfln Duration pH
YPF DiflnYPF (mg) (mg) Weight (Days) (S.U.)
1 Mississippi River at McCalllster Grain near Elaine
14-May-91 W
14-May-91 W
16-Jul-91 W
16-Jul-91 W
10-Sep-91 W
10-Sep-91 W
19-Nov-91 W
19-Nov-91 W
07-Jan-92 W
07-Jan-92 W
11-Mar-92 W
11-Mar-92 W
C 0
P 7
C 10
P 7
C 0
P 7
C 10
P 0
C 0
P 7
C 10
P 3
0 (
10 (
0 (
10 (
0 (
7 (
10 (
0 (
0 (
7 (
0 (
3 (
3 "
3
3 I6'3
3
3 18-1
3
3 13-3
3
3 io-i
3
3 "
3
16.2 O

15.9 O

16.4 O

13.7 O

12.2 (§)

16.9 O


0.595 0.537

0.564 0.493

0.777 0.844

0.657 0.64

0.336 0.273

0.437 0.469
7
O 7
7
O 7
7
O 7
7
O 7
7
O 7
7
O 7
7.2
7.2
7.7
7.7
8.0
8.0
8.1
8.1
7.4
7.4
6.8
6.8
2 Mississippi River at McCallle Access near Arkansas City
14-May-91 W
14-May-91 W
16-Jul-91 W
16-Jul-91 W
10-Sep-91 W
10-Sep-91 W
19-Nov-91 W
19-Nov-91 W
07-Jan-92 W
07-Jan-92 W
11-Mar-92 W
11-Mar-92 W
C 0
P 0
C 10
P 3
C 0
P 3
C 10
P 0
C 10
P 3
C 0
P 7
0 (
10 (
0 (
10 (
0 (
7 (
10 (
0 (
0 (
7 (
0 (
3 (
3 ".1
3
3 16
3
3 17
3
3 12-6
3
3 1l>-2
3
3 17.6
3
16.2 O

15.9 O

16.4 O

13.7 O

12.2 (§)

16.9 O


0.54 0.537

0.534 0.493

0.773 0.844

0.658 0.64

0.297 0.273

0.422 0.469
7
O 7
7
O 7
7
O 7
7
0 7
7
O 7
7
0 7
6.9
6.9
7.3
7.3
7.5
7.5
8.1
8.1
6.5
6.5
6.5
6.5
S-31 9 Mississippi River Midstream at Plaquemlne Ferry Landing East ofPlaquemlne
14-May-91 W
14-May-91 W
11-Jun-91 W
11-Jun-91 W
Sample Types Test Species
C 0
P 0
C 0
P 3
0 (
10 (
0 (
0 (
3 I4-7
3
3 15-8
3
16.2 O

16.8 O


0.562 0.537

0.677 0.695
7
O 7
7
O 7
7.2
7.2
7.9
7.9
Agency EPA Reach Latitude Longitude County State
Total Total Unionized
Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-N
(mg/l) (mg/L) (umhos/cm) (mg/L) (mg/L) (mgfl)
ADPCE
102
102
132
132
130
130
120
120
88
88
154
154
ADPCE
94
94
139
139
130
130
141
141
67
67
97
97
LDEQ
84
84
92
92

156 202
156 202
183 401
183 401
164 372
164 372
130 393
130 393
51 189
51 189
127 254
127 254
08030202002
124 205
124 205
188 411
188 411
172 358
172 358
162 445
162 445
35 141
35 141
134 300
134 300
08070100007
112 266
112 266
128 282
128 282
34'22W 90*45'55"
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
33«39-20- 90*1 2'43-
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
SOMTW 91'12'Sr
< 0.1
< 0.1
< 0.1
< 0.1
Phillips AR
0.6
0.6
0.1
0.1
0.7
0.7
0.2 < 0.1
0.2 < 0.1
0.4 < 0.1
0.4 < 0.1
0.5 < 0.1
0.5 < 0.1
Desha AR
0.2
0.2
0.1
0.1
0.2
0.2
0.4 < 0.1
0.4 < 0.1
0.6 < 0.1
0.6 < 0.1
0.4 < 0.1
0.4 < 0.1
Iberv/lle LA
0.5
0.5
0.6
0.6
Significant Effects
W=Water     C= Ceriodaphnia dubia  S= Cyprinodon variagatua   (•) Significant Difference     Crustaceans - mortality.
S=Sediment    P= Pimephalas promotes 0= Daphni* pulex        (_) No Significant Difference   Fish - mortality, teratogenicity, or abnormal swimming behaviour.
YPF - average number of young per female - applicable only to  Ceriodaphnia dubia.
Weight • applicable only to fish larval survival and growth test
                                                      15

-------
Station Number Waterbody Description
  Agency  EPA Reach    Latitude Longitude      County   State
Sample Test
Data of Collection Type Sptclts
Test
Percent
Effected
Control
Percent
Effected
Test Control Significant
Significant Test Control Significant Weight Weight Dtf In
Effects YPF YPF DtflnYPF (mg) (ing) Weight
Test
Duration pH
(Days) (S.U.J
S-51 Mississippi River at Belle Chasse near West Bank Ferry Landing
16-Jul-91 W C
16-Jul-91 W P
0
0
0
10
814.8 15.9

O


0.533 0.493

0
7
7
7.7
7.7
S-52 Mississippi Rhw near East Bank Ferry Utntfng across Irorn Bell Chasse
13-Aug-91 W C
13-Aug-91 W P
10-Sep-91 W C
10-Sep-91 W P
150ct-91 W C
15-Oct-91 W P
19-Nov-91 W C
19-NW-91 W P
11-Dac-91 W C
11-D«c-91 W P
07-Jan-92 W C
07-Jan-92 W P
12-Feb-92 W C
12-Fab-92 W P
11-Mar-92 W C
11-Mar-92 W P
08-Apr-92 W C
OS-Apr-92 W P
13-May-92 W C
13-May-92 W P
10
10
0
0
0
0
20
13
0
17
10
13
0
7
0
3
0
0
10
10
10
17
0
7
0
3
10
0
0
17
0
7
0
7
0
3
0
0
10
10
O 14.8 16-2
Q
Q 18.7 16.4
Q
Q 16.3 16.1
Q
Q 9 13.7
Q
Q 15.3 19
Q
Q 9-2 12.2
Q
Q 17.5 18.3
Q
Q 17.8 16.9
Q
Q 19.2 20.3
fj
Q 20.3 19.1
o
0

0

o

(§)

o

®

o

o

o

o


0.372 0.343

0.814 0.844

0.522 0.448

0.709 0.64

0.602 0.602

0.302 0.273

0.361 0.38

0.407 0.469

0.412 0.448

0.321 0.305

o

o

o

0

o

0

o

0

o

o
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
8.1
8.1
8.4
8.4
7.6
7.6
7.9
7.9
7.6
7.6
7.9
7.9
7.7
7.7
7.3
7.3
7.3
7.3
7.1
7.1
S-53 Mississippi Rrver near West Bank Ferry Landing at Placfjemlne
11-Jun-91 W C
11-Jun-91 W P
0
0
0
0
Q 17.1 16.8
0
0.678 0.695
o
7
7
7.7
7.7
S-54 Mississippi River near East Bank Ferry Landi ng across from Ptaquomtne
14-May-91 W C
14-May-91 W P
16-Jul-91 W C
16-Jul-91 W P
Sample Types Test Species
0
0
20
7
0
10
0
10
Q 15.5 16.2
Q
Q 14.4 15.9
O
o

o


0.55 0.537

0.528 0.493

o

o
7
7
7
7
7.5
7.5
7.1
7.1
Total Total Unionized
Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-N
(man.) (mg/t) (umhosfcm) (mg/L) (rngfl.) (mg/|)
LDEQ
122
122
LDEQ
139
139
130
130
130
130
105
105
88
88
99
99
119
119
101
101
84
84
104
104
LDEQ
96
96
LDEQ
84
64
130
130

167 389
167 389
08090301040
188 393
188 393
180 461
180 461
393 478
393 478
138 453
138 453
119 346
119 346
127 283
127 283
103 367
103 367
138 345
138 345
131 273
131 273
163 266
163 266
08070100007
120 292
120 292
08070100007
104 274
104 274
175 385
175 385
29-5ri1" 89'58'SO-
< 0.1
< 0.1
29*5ff54- 89'5ff41"
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
so-iT-or gnur
< 0.1
< 0.1
30M7W 9rir45-
< 0.1
< 0.1
< 0.1
< 0.1
Flaquemlnes LA
0.2
0.2
ttwrvttte LA
0.4
0.4
0.6
0.6
0.4
0.4
0.5 < 0.1
0.5 < 0.1
0.4
0.4
0.6 < 0.1
0.6 < 0.1
0.5 < 0.1
0.5 < 0.1
0.5 < 0.1
0.5 < 0.1
0.5 < 0.1
0.5 < 0.1
2.4 < 0.1
2.4 < 0.1
Hienaie LA
0.7
0.7
Jbarvttte LA
0.5
0.5
0.4
0.4
Significant Effects
W-Water      C= Cenbdap/im. dob*   S= CyfrinoOon varwoaftu    MM Significant Difference      Crustaceans - mortality.
S=Sedlment    P= Pmaphmhi pmmafu D= Depnnfe pufex          Q No Significant Difference   Fish - mortality, tentogenicity, or abnormal swimming behaviour.
YPF-average number of young per female-applicable only to   Cenbdap/infe duUa.
Weight - applicable only to fish larval survival and growth test
                                                                 16

-------
Station Number Watertody Description
  Agency  EPA Reach    Latitude  Longitude     County    State
Date of Collection
Continued...
13-Aug-91
13-Aug-91
10-Sep-91
10-Sep-91
15-Oct-91
15-0ct-91
19-Nov-91
19-Nov-91
11-Dec-91
11-Deo-91
07-Jan-92
07-Jan-92
12-Feb-92
12-Feb-92
11-Mar-92
11-Mar-92
08-Apr-92
OS-Apr-92
13-May-92
13-May-92
UPR21 1.001525
24-Sep-90
24-Sep-90
UPR21 1.001535
24-Sep-90
24-Sep-90
UPR21 1.001536
24-Sep-90
24-Sep-90
UPR211.001537
24-Sep-90
24-Sep-90
Sample Test
Type Species
Test Control
Percent Percent
Effected Effected
Test Control Significant
Significant Test Control Significant Weight Weight Dlfln
Effects YPF YPF DrHnYPF (mg) (mg) Weight
Test
Duration
(Days)
pH
(S.U.)
Alkalinity Hardness Conductivity
(mg/L) (mg/L) (umhosfcm)
Total Total
Chlorine Ammonla-N
(mgfl.) (mg/L)
Unionized
Ammonla-N
(mgfl)
Mississippi River near East Bank Ferry Landing across from Plaquemlne _, .
W C
W P
W C
W P
W C
W P
W C
W P
W C
W P
W C
W P
W C
W P
W C
W P
W C
W P
W C
W P
0
7
0
10
0
0
0
3
0
7
10
17
0
3
0
7
0
13
0
17
10
17
0
7
0
3
10
0
0
17
0
7
0
7
0
3
0
0
10
10
Q 15.6 16.2 O
Q 0.329 0.343
Q 18-3 16.4 O
Q 0.908 0.844
Q 15.7 16.1 O
Q 0.514 0.448
Q 11.9 13.7 O
Q 0.669 0.64
Q 16 19 O
Q °-593 0.602
Q 1° 12.2 (•)
Q 0-27 0.273
Q 17.4 18.3 O
Q 0.381 0.38
Q 19 16.9 O
Q 0-408 0.469
Q 22.6 20.3 O
Q 0.337 0.448
Q 21.6 19.1 O
O 0-321 0.305

O

O

O

O

O

O

O

O

(§)

O
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
8.3
8.3
8.3
8.3
7.9
7.9
8.0
8.0
7.5
7.5
7.7
7.7
7.9
7.9
7.6
7.6
6.7
6.7
7.2
7.2
Galllnas River below WWTP
W C
W P
0
7
10
3
O 18.5 17.2 O

7
7
8.0
8.0
Ga///nas River above WWTP
W C
W P
0
10
10
3
816.3 17.2 O



7
7
8.0
8.0
Pacos Arroyo above Galllnas River
W C
W P
0
0
10
3
O 16 17.2 O
O


7
7
7.9
7.9
Spring Arroyo at Airport Road
W C
W P
100
30
10
3
Sample Types Test Species


Significant Effects



7
7

7.9
7.9

140 179
140 179
130 164
130 164
139 172
139 172
88 150
88 150
90 107
90 107
101 134
101 134
113 142
113 142
101 142
101 142
82 126
82 126
104 167
104 167
NMED
231 452
231 452
NMED
226 476
226 476
NMED
186 582
186 582
NMED
211 280
211 280

388
388
376
376
431
431
426
426
310
310
276
276.
353
353
350
350
264
264
297
297

785
785

851
851

963
963

449
449

< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
« 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1

< 0.1
« 0.1

< 0.1
« 0.1

« 0.1
< 0.1

< 0.1
< 0.1

0.2
0.2
0.5
0.5
0.5
0.5
0.8
0.8
0.2
0.2
0.4
0.4
0.8
0.8
0.1
0.1
0.4
0.4
0.4
0.4

0.5
0.5

0.5
0.5

0.5
0.5

1.2
1.2







< 0.1
< 0.1


< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
NM

NM


NM


NM



W=Water      C= Cenxlaphnm dubm   S= Cyprinodon twfegalus    (•) Significant Difference      Crustaceans - mortality.
S=Sediment    P= Pimephalos promotes D= Daphni* puhx          Q No Significant Difference   Fish • mortality, teratogenicity, or abnormal swimming behaviour.
YPF - average number of young per female - applicable only to   Coriod»phnit dubm.
Weight - applicable only to fish larval survival and growth last
                                                                  17

-------
Station Number Waterbody Description
  Agency  EPA Reach    Latitude  Longitude     County   State
                                      Tort     Control                                        Test    Control  Significant    Tast                                                Total        Total        Unionized
                  Sample  Tart       Parcant    Paicant   Slgnlflcant   Tact  Control  SlgnMcant Walght   Walght    OH In     Duration    pH     Alkalinity  Hardnass Conductivity   Chlorlna     Ammonla-N     Ammonla-N
DataofCollactlon    Typa  Spaclaa      Effactad    EfteM    Effects     YPf    YPF   DHInYPF  (mg)     (mg)     WalgM     (Dayt)    (S.U.)     (mgfl.)    (mflfl.)   (umhoalcin)     (rng/L)       (mgft.)         (mgfl)
UPR211.001538
24-Sep-90
24-Sep-90
UPR21 1.001539
24-Sep-SO
24-Sep-90
URO119.023505
25-Jul-89
25-Jul-89
URO1 19.023510
25-Jul-89
25-JUI-89
URG119.023515
25-Jul-89
25-Jul-89
URG1 19.023535
25-Jul-a9
25-Jul-89
OB002
20-Mar-90
20-Mar-90
17-Sap-90
11-Dec-90
11-Oec-90
20B
23-Jul-90
23-Jul-90
30-0c<-90
Pecos Arroyo at Harris Lake
W C 20
W P 3
Galllnas River at Grand
W C 60
W P 13

10
3

10
3

O 15.4 17.2

(5) 0 17.2

O

(§)

7
7

7
7

8.0
8.0

8.3
8.3
Rkt Pueblo de Taoa at the Rio Grande
W C 0
W P 10
0
3
O 18 19.2
O
7
7
8.6
8.6
Rto Puebto de Taoa. Upper End of the Lower Canyon
WC 0
W P 7
Rto Puebto de Taoa Below the Taot
W C 0
W P 13
0
3
O 20.7 19.2
O
7
7
8.4
8.4
i WWTF and Above USGS Guage
0
3
O 15.8 19.2
O
7
7
8.3
8.3
Rto Pueblo de Taoa above the Taoa Waatewater Treatment Facility
W C 0
W P 10
0
3
8 '" ~
0
7
7
8.2
8.2
Bird Creek at Seth Street North Bridge In Tulsa
W C 0
W P 0
W C 40
W C 0
W P 7
Tar Creek at 22nd Avenue hi Miami
W C 100
W P 100
W C 100
Sample Tvoat Taat Specie*
W=Watar O Carfodaonnia dubm S= Cvorinodan vm
0
0
10
10
7
O 18 17.7
O 7.2 14.1
Q 20.4 19.4
0
7
7
7
7
7
7.4
7.4
7.3
7.0
7.0
NMED
148 472
148 472
NMED
246 384
246 384

843
843

562
562
NMED 13020101013
194 253
194 253
450
450
NMED 13020101013
217 273
217 273
470
470
NMED 13020101013
231 286
231 286
492
492
NMED 13020101013
233 292
233 292
499
499
WCOG 11070107001
74 98
74 98
84 120
83 152
83 152
189
189
383
534
534
OWRB 11070206014
0
3
0
ieoatus



Siflnificant Effects
Ciuttaceant - mortalitv.
1
1
1

4.3
4.3
5.9

500 2000
500 2000
12 2000
YPF - averaoa numb
1980
1980
2050
Brof vouna

< 0.1
< 0.1

< 0.1
< 0.1

0.7
0.7

0.5
0.5
36-20-20- 105M3M5- Taos
< 0.1
< 0.1
0.6
0.6
NM

NM

NM

36*21'ir 105*4?ir Taoa NM
< 0.1
< 0.1
0.7
0.7
36*22438- 105'40'Or Taoa
« 0.1
< 0.1
1.6
1.6
36'22-49- 105-39-44" Taoa
< 0.1 <
< 0.1 <
36M4W 95M9-50-
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
X'SSSSr 94'52'OS"
< 0.1
< 0.1
< 0.1
ner femala - aoolieabl* on
0.1
0.1
Tulsa
0.5
0.5
0.2
1.7
1.7
Oltowa
1.2
1.2
0.5
h/to Cmriodfnhn

NM

nMv

OK

OK

fcduMe.
                                                           (_) No Significant Difference    R*h - mortality, toratoganicity, or abnormal cwimming behaviour.
Weight - appllcabla only to Man larval tutvival and growth teat

                                                                  18

-------
 Station Number  Waterbody Description
                  Sample   Test
Date of Collection     Typt Species
                     Ttst
                    Percent
                                                Effected

Continued.-     Tar Creek at 22nd Avenue In Miami
Control                                         Test   Control  Significant    Test
Percent   significant    Test  Control  Significant Weight  Weight    Wf In     Duration
                                                                                                                   pH
                                          Effects      YPF    YPF    DtflnYPF   (mg)     (mg)     Weight     (Days)   (S.U.)
    Agency  EPA Reach    Latitude  Longitude     County   State

                                   Total        Total        Unionized
Alkalinity  Hardness Conductivity    Chlorine    Ammonla-N     Ammonla-N
  (rngfl.)    (mgfl.)   (umhos/cm)     (mg/L)       (mfl/|j          (mg/l)
30-Jan-91
30-Jan-91
7
23-Jul-90
23-Jul-90
30-Od-90
30-Jan-91
30-Jan-91
W
W
C
P
100
100
0
0


1
1
6.1
6.1
Tar Creek at Oklahoma/Kansas State Line North of Miami
W
W
W
W
W
C
P
C
C
P
100
100
100
100
100
0
0
0
0
0
©
©
©
©
®
1
1
1
1
1
7.3
7.3
7.4
6.0
6.0
51
51
OWRB
115
115
118
49
49
640
640
957
957
11070206014
906
906
960
280
280
1259
1259
1496
443
443
< 0.1
< 0.1
36'59i54" 94'5V05"
< 0.1 <
< 0.1 <
< 0.1
< 0.1
< 0.1
15
15
Ottawa
0.1
0.1
0.1
0.2
0.2


OK





 OCC1
Lake Creek at SH152 Bridge above Fort Cobb Reservoir
                                                                                                                                                  OCC   11130302037  35'1724"   98'31'44"
                                                                                                                                                                                                    Cadbb
                                                                                                                                                                                                               OK
14-May-90
14-Aug-90
14-Aug-90
10-Sep-90
24-Sep-90
24-Sep-90
21-0ct-90
21-Oct-90
OCC2
09-Apr-90
09-Apr-90
15-Apr-90
14-Aug-90
14-Aug-90
10-Sep-90
16-Oct-90
16-Od-90
21-Oct-90
21-Oct-90
24-Feb-91
24-Feb-91
28-Feb-91
28-Feb-91
28-Feb-91
W
W
W
S
W
W
S
S
C
C
P
D
C
P
D
P
0
0
13
0
0
7
0
3
Galnes Creek near Lake Eufala,
W
W
S
W
W
S
W
W
S
S
W
W
W
W
S
C
P
D
C
P
D
C
P
D
P
C
P
C
P
C
0
10
0
0
3
0
0
3
0
7
0
7
60
7
40
0
0
3
0
10
3
0
3
Q 18.5 15.2
Q 18.7 17.2
o
Q
Q 18.1 17.2
o
Q
o
8


O



7
7
7
2
7
0
2
7
8.3
7.9
7.9
8.0
8.2
8.2
7.5
7.5
abouf 1.25 Miles Northwest of Gotten
0
7
0
0
3
0
0
10
0
3
0
3
10
0
10
O 17.6 16
Q
Q
Q 16.8 175
o
Q
Q 19-6 18
Q
Q
Q
Q 15.7 17.7
Q
© 9 18.1
Q
O 9-4 18.1
Sample Types Test Species
O


O


o



o

©

©
Significant Effects
7
7
2
7
7
2
7
7
2
7
7
7
7
7
7

6.4
6.4
7.9
6.6
6.6
6.9
6.4
6.4
6.6
6.6
5.3
5.3
6.1
6.1
6.5

275
233
233
145
251
251
137
137
OCC
7
7
40
10
10
108
2
2
180
180
1
1
1
1
172

290
269
269
124
252
252
128
128
600
485
485
338
339
339
308
308
11090204050
32
32
57
24
24
100
28
28
144
144
56
56
60
60
225

80
80
257
48
48
284
82
82
356
356
201
201
153
153
500

< 0.1 <
< 0.1
« 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
34'54'OS- 95*2922"
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
« 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
« 0.1
< 0.1
< 0.1
< 0.1

0.1
0.4
0.4
0.7
0.7
0.7
0.7
0.7
Latlmer OK
0.5
0.5

0.4
0.4
2.4
0.4
0.4
1.9
1.9
9.6
9.6
2.2
2.2
2.6

W=Watof      C=Coriodaphniadubia  S= Cyprinodon variogatui    © Significant Difference      Crustaceans - mortality.
SsSediment    P= Pimephfha prometai D* Daphnia pu/sx          (_) No Significant Difference    Fish - mortality, teratogenicity, or abnormal swimming behaviour.
                                                                                                                             YPF - average number of young per female - applicable only to  Ceriodaphnia dubia.
                                                                                                                             Weight - applicable only to fish larval survival and growth test

                                                                                                                                                                                               19

-------
Station Number Waterttody Description
Agency  EPA Reach    Latitude Longitude    County   State
Date of Collection
Continued...
28-Feb-91
16-Jul-91
16-Jut-91
2cXlul-91
28^>ul-91
OCC3
2ftOul-91
28-JuU91
OCC4
28-Juk91
28-Jul-91
Test Control Test Control Significant
Sample Test Percent Percent Significant Test Control Significant Weight Weight Dlf In
Type Species Effected Effected Effects YPF YPF DiflnYPF (mg) (ing) Weight
Galnes Creek near Lake Eufala, about 125 Miles Northwest of Gowen
S P
W C
W P
S C
S P
10
0
17
50
20
0
0
10
0
10
Q
Q 14.6 15.9
o
® 8.8 14.9
o

o
0.553 0.493 Q
(•)

Test
Duration pH
(Days) (S.U.)
7
7
7
7
7
6.5
6.2
6.2
6.4
6.4
Lake Eufala Northwest ofHwy 31 Bridge
S C
S P
0
7
0
10
815.4 14.9

O

7
7
6.2
6.2
Lake Eufala atAdamaon Road f 0.5 Mites EastofMcAlester
S C
S P
10
7
0
10
817.6 14.9

o

7
7
6.6
6.6
Total Total Unionized
Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonia^
(mgfl.) (mgfl.) (umhos/cm) (mgfl.) (mg/L) (mgfl)
172 225
27 75
27 75
109 104
109 104
500 < 0.1
230 < 0.1
230 < 0.1
324 < 0.1
324 < 0.1
OCC 22060050010 34*58'39* 95'3T43"
90 92
90 92
279 < 0.1
279 < 0.1
OCC 22060050010 34*5578' 95*34'16"
210 175
210 175
2.6
0.6
0.6
1.1
1.1
Ptttsrjurg
1.7
1.7
Ptttsburg





OK


OK
450 < 0.1 12.5
450 < 0.1 12.5
OCC5
              Pitt Creek 2 Miles South of US 270 3.5 Miles East ofHartshome
                                                                                                                            OCC   22060040040  34*5ri4'
                                                                                                                                                                     LaUmer    OK
27-Feb-91
27-Fab-91
28-Fab-91
28-Feb-91
16-Jul-91
16-JUI-91
26-Jul-91
26-Jul-91
W
W
S
S
W
W
S
S
C
P
C
P
C
P
C
P
100
100
100
100
100
100
100
100
o (
o (
0 (
o (
0 (
o (
0 (
0 (
-v
)
'
.
)
)

.
.
.
*
j :
3.1
3.1
3.7
3.7
2.7
2.7
3.8
! 3.8
0
0
0
0
0
0
0
0
0
0
231
231
650
650
238
238
1977
1977
684
684
1980
1980
681
681
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
16.8
16.8
2.6
2.6
15.9
15.9
4.8
4.8
OCC6
              Deep Fork One Mile East oflH 35, One Mile South ofH4SE ofEdmond
 OCC   52071002001   35*34'4r  97*2539*    Oklahoma    OK
07-May-91 W C
07-May-91 S P
uX-Jun-91 S C
04-Jun-91 S P
OS-Nov-91 W C
05-Nov-91 W P
13-Nov-91 S C
13-N0V-91 S P
0
3
10
7
10
3
0
7
20
10
0
3
0
3
0
0
Q 18-3 15.6
Q
Q 14.9 15.5
(~)
Q 13 12
Q
Q 11-3 10.5
o
O

o

o

0

7
7
7
7
7
7
7
7
7.9
7.9
7.6
7.6
8.4
8.4
7.5
7.5
OCC7 Spring Creek West of OS and South of US77 East of Edmond
07-May-91 W C
Sample Types Test Species
W=Water C= Carfedapnnile dub*
100

8s Cyorinoda

10


iimtr
®

V^5' Stflnincent Difference
(1 MA CisinHlf.n+ HiM«w«

Significant Effect*
Crustacean* - mortality.
1


7.8


236
236
224
224
286
286
269
269
260
260
180
180
315
315
221
221
419
419
603
603
783
783
434
434
OCC 52071002003
250

YPF-
320

594

< 0.1 0.4
< 0.1 0.4
< 0.1 0.9
< 0.1 0.9
< 0.1 0.7
< 0.1 0.7
< 0.1 1.3
< 0.1 1.3
35*2ff4r 97*25'30"
< 0.1 0.5

average number of young per female - applicable only to








Oklahoma OK


Caribdap/mfa dub*
                                                                                                                                                                                20

-------
Station Number Watertody Description
Test
Sample Test Percent
Date of Collection Type Species Effected
Continued.^
04-Jun-91
04-Jun-91
OS-Nov-91
OS-Nov-91
13-Nov-91
13-Nov-91
ODEQ10
08-Apr-92
08-Apr-92
08-Apr-92
08-Apr-92
12-May-92
12-May-92
20-Aug-92
20-Aug-92
20-Aug-92
20-Aug-92
12-Oct-92
12-Oct-92
ODEQ12
08-Apr-92
08-Apr-92
08-Apr-92
08-Apr-92
20-Aug-92
20-Aug-92
20-Aug-92
20-Aug-92
12-Oc)-92
12-Oct-92
ODEQ2
15-Mar-90
18-Mar-90
Control Test Control Significant Test
Percent Significant Test Control Significant Weight Weight Dlfln Duration pH
Effected Effects YPF YPF Dlfln YPF (mg) (ing) Weight (Days) (S.U.)
Spring Creek Westof I3S and South of US77 East of Edmond
S C 0 0 Q 14.5 15.5
S P
W C
W P
S C
S P
Acid Creek
W C
W P
S C
S P
W C
W P
W C
W P
S C
S P
W C
W P
13
60
3
10
3

0
13
0
7
10
100
0
3
10
3
0
10
3 Q
0 ® 7.8 12
3 Q
0 Q 12.7 1°-s
o O

0 Q 22.4 20.3
10 Q
10 Q 3.3 13.7
7 Q
10 Q 16 19-1
0 Q 17 15.7
3 Q
0 Q 15.4 15.7
3 Q
0 Q 15.2 15.1
7 O
O

®
O


o

®
o
0
0
o

7
7
7
7
7
7

7
7
7
7
7
7
7
7
7
7
7
7
7.5
7.5
8.3
8.3
7.8
7.8

8.3
8.3
6.9
6.9
8.2
8.2
7.8
7.8
7.9
7.9
8.1
8.1
Tributary of Chtckaskla River near Blackwell
W C
W P
S C
S P
W C
W P
S C
S P
W C
W P
50
3
30
10
100
3
100
100
100
100
0 ® 8.2 20.3
10 Q
10 Q 11.1 13.7
7 Q
0 ®
3 Q
0 ®
3 ®
0 ®
7 ®
®

®






7
7
7
7
4
7
2
7
1
7
7.4
7.4
7.8
7.8
6.8
6.8
7.6
7.6
6.8
6.8
Clmarron River North of Lone Mountain
W S
S D
100
0


Sample Types Test Species
W=Water
S= Sediment
C= Coriodaphnia dubia
P- Prmaonatos oromafe*
S= Cyprinodon varfogafus ® Significant Difference
D= Deo/wife Dufax O No Significant Difference


Significant Effects
Crustaceans - mortality.
Fish - mortality, teratogenicitv. or at
7
2

7.9
8.1

Agency EPA Reach Latitude Longitude County State
Total Total Unionized
Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-fl
(mg/L) (mg/L) (umhos/cm) (man.) (mg/t) (mg/l)
212 172
212 172
256 299
256 299
214 221
214 221
ODEQ
142 228
142 228
168 145
168 145
362 371
362 371
122 166
122 166
182 199
182 199
136 167
136 167
ODEQ
300 665
300 665
212 233
212 233
78 77
78 77
158 146
158 146
62 142
62 142
ODEQ
0 1000
284 196

546
546
738
738
475
475

930
930
504
504
1030
1030
456
456
425
425
615
615

1515
1515
472
472
456
456
447
447
463
463

1673
950

< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
36'4ff52- 97'03'54"
« 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
« 0.1
< 0.1
< 0.1
« 0.1
36'48'33- 97M7-1 1"
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
36-2T22- 98Mr57"
< 0.1
< 0.1 <

1.2
1.2
1.3
1.3
0.6
0.6

0.5
0.5
1.9
1.9
0.3
0.3
0.4
0.4
1.8
1.8
0.4
0.4

1.3
1.3
3.2
3.2
0.4
0.4
0.6
0.6
0.2
0.2

0.4
0.1

YPF - average number of young per female - applicable only to
'normal swimming behaviour. Weiaht - aoolicable only to fish larval survival and arowth test
••*•• •




Kay OK
t







Kay OK









Major OK



Ceriodaphnia dubia..
21

-------
Station Number  Waterbody Description
  Agency  EPA Reach     Latitude Longitude     County   State
Sample Test
Date of Collection Type Species
Continued...
18-Mar-90
09-May-90
18-Jul-90
24-Jul-90
24-Jul-90
10-Od-90
07-Apr-91
07-Apr-91
IS-May-91
26-AU0-91
12-Nov-91
30-Mar-92
30-Mar-92
05-May-92
12-Aug-92
12-Aufl-92
08-Oct-92
OOEQ3
05-Mar-90
05-Mar-90
11-Mar-90
11-Mar-90
07-May-90
07-May-90
11-Jul-90
11-Jul-90
25-Jul-90
25-Jul-90
03-Oct-90
24-Mar-91
24-Mar-91
24-Mar-91
24-Mar-91
07-May-91
07-May-91
Sample Types
Test
Effected
Control Test Control Significant Test
Percent significant Test Control Significant Weight Weight Wf In Duration pH
Effected Effects YPF YPF DtflnYPF (mg) (mg) Weight (Days) (S.U.)
Alkalinity
(mg/L)
Hardness
(man.)
Conductivity
(umhosfcm)
Total Total Unionized
Chlorine Ammonla-N Ammonla-N
(mfln.) (mg/L) (mgfl)
dmarron River North of Lone Mountain
S
W
W
S
S
W
W
S
W
S
W
W
S
W
W
S
W
P
S
S
D
P
S
S
S
S
S
S
S
S
S
S
S
S
10
100
100
0
3
100
100
10
100
10
100
100
0
100
100
13
100
3 Q 7
0 © 9
7 ® 8
0 Q 2
3 Q 7
3 ® 8
0 © 6
7 Q 8
0 © 6
3 Q 8
0 © 6
3 © 6
3 Q 9
3 © 5
0 © 7
3 O 7
3 ® 7
8.1
8.4
8.1
8.3
8.3
8.0
7.9
7.2
7.9
8.0
8.0
8.0
8.0
7.9
7.9
7.5
7.8
Coal Creek near Henryetta
W
W
S
S
W
W
W
W
S
S
W
W
W
S
S
W
W
Test Species
c
P
0
P
c
P
c
P
0
P
c
c
P
c
P
c
P

100
100
100
37
100
20
100
100
100
100
100
100
100
100
100
100
100

0 (
0 (
0 (
10 (
0 (
0 (
0 (
0 (
0 (
0 (
0 (
0 (
0 (
0 (
0 (
0 (
0 (
) 1
) 2
) 1
) 7
) 1
) 5
) 1
)
5
;
1
)
5
> 1
) 7
) 1
) 2
Significant Effect*
5.3
5.3
7.9
7.9
62
6.2
6.3
6.3
6.7
6.7
5.9
6.2
6.2
6.5
6.5
6.1
6.1

284
198
166
187
187
131
176
347
146
239
195
179
368
156
116
182
102
196
0
1097
244
244
1000
0
0
1000
3000
2000
1450
138

870
2000

950
10160
17100
1737
1737
21300
38200
29840
40700
32500
25700
37700
6200
27000
23600
25500
26500
OOEQ 3!
4
4
138
138
22
22
5
5
84
84
4
28
28
216
216
12
12

1000
1000
165
165
600
600
600
600
1503
1503
1000
1000
1000
370
370
1000
1000

1311
1311
840
840
1210
1210
1613
1613
2080
2080
1423
1006
1006
723
723
1205
1205

< 0.1 <
< 0.1
< 0.1
< 0.1
« 0.1
< 0.1
< 0.1 <
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
J^TW 95'sror
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1

0.1
1.0
0.7
0.5
0.5
0.1
1.0
0.5
1.0
72
1.2
0.7
1.9
02
1.9
22
024
OtanOgM OK
1.0
1.0
0.4
0.4
2.0
2.0
3.0
3.0
1.3
1.3
0.7
0.4
0.4
0.4
0.4
0.5
0.5

W=W«ter      OCenboaphnfcduMa   S= Cyprinodon ranogefus    ® Significant Difference      Cfuctaceans - mortality.
S=Sediment    P= PlrmpnaJn pmmelti D= Dap/into potot          O N° Significant Difference   R*h - mortality, temtogenicity, or abnormal swimming behaviour.
YPF-average number of young per female-applicable only to   Canbdapnnfc duMa.
Weight - applicable only to fish larval survival and growth last
                                                                 22

-------
Station Number Waterbody Description
Test
Sample Test Percent
Date of Collection Type Species Effected
Continued...
07-Aug-91
07-Aug-91
13-Aug-91
13-Aug-91
05-Nov-91
OS-Nov-91
ODEQ4
05-Mar-90
05-Mar-90
11-Mar-90
11-Mar-90
11-Jul-90
11-Jul-90
24-Jul-90
24-Jul-90
ODEQ6
09-Apr-91
09-Apr-91
14-Apr-91
14-Apr-91
28-May-91
28-May-91
14-Aug-91
20-Aug-91
20-Aug-91
ODEQ7
24-Mar-91
24-Mar-91
24-Mar-91
24-Mar-91
07-May-91
07-May-91
07-Aug-91
Control Test Control Significant Test
Percent Significant Test Control Significant Weight Weight Dlf In Duration
Effected Effects YPF YPF Dlf In YPF (mg) (mg) Weight (Days)
pH
(S.U.)
Agency EPA Reach Latitude Longitude County State
Total Total Unionized
Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-N
(mg/L) (mg/L) (umhosfcm) (mg/L) (mgfl.) (mgfl)
Coal Creek near Henryetta
W
W
S
S
W
W
C
P
C
P
C
P
10
17
100
100
100
100
10 Q 17.4 17-2 O
10 Q
0 ®
0 (•)
0 (8)
0 (•)
7
7
3
3
1
2
7.5
7.5
6.2
6.2
5.1
5.1
Lake Francis at Upper End
W
W
S
S
W
W
S
S
C
P
D
P
C
P
D
P
0
7
0
3
10
3
0
0
0 Q 15.6 13.8 O
7 Q
0 Q
10 Q
0 Q 8-9 12 (§)
3 Q
0 Q
3 O
7
7
2
7
7
7
2
7
7.3
7.3
8.4
8.4
7.9
7.9
7.2
7.2
Bitter Creek
W
W
S
S
W
W
S
W
W
C
P
C
P
C
P
C
C
P
0
10
0
13
0
7
0
100
20
10 Q 16-3 15.8 O
7 Q
10 Q 19.6 15.1 O
3 Q
0 Q 13.8 13.6 O
3 Q
0 Q 16 14.4 O
0 ®
7 O
7
7
7
7
7
7
21
1
7
8.0
8.0
7.5
7.5
7.5
7.5
7.8
8.5
8.5
Tributary of San Bols Creek
W
W
S
S
W
W
W
C
P
C
P
C
P
C
100
100
100
100
100
100
0
Sample Types Test Species
0 ( )
0 ( )
0 ( )
0 ( )
0 ( )
0 Q
10 O 16-2 17.2 O
Significant Effects
1
7
1
7
1
2
7

6.6
6.6
6.6
6.6
6.2
6.2
6.4

116
116
92
92
21
21
ODEQ
82
82
120
120
100
100
127
127
146
146
175
175
1925
1925

107
107
184
184
114
114
124
124
532
532
514
514
1471
1471

162
162
574
574
181
181
379
379
ODEQ
196
196
244
244
198
198
189
206
206
1421
1421
317
317
832
832
348
2150
2150
4990
4990
997
997
2530
2530
1136
6490
6490
ODEQ
23
23
160
160
30
30
32

32
32
181
181
52
52
12

78
78
1062
1062
89
89
156

0.1
0.1
« 0.1
< 0.1
< 0.1
< 0.1
36'0275" 94'33'33"
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1 <
< 0.1 <
< 0.1
< 0.1
34'35'38- aa-23fM'
* 0.1
< 0.1
< 0.1
< 0.1
< 0.1
« 0.1
< 0.1
< 0.1
< 0.1
37'05130- 95*21'05"
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
0.1

0.6
0.6
2.2
2.2
1.4
1.4
Adalr OK
0.4
0.4
2.6
2.6
0.1
0.1
0.2
0.2
Jackson OK
0.4
0.4
3.6
3.6
1.2
1.2
1.7
0.6
0.6
Plttsburg OK
0.2
0.2
0.2
0.2
0.5
0.5
0.1

W=Water       O Ctriodaphnia dubia   S= Cyprinodon varitgalua     (*) Significant Difference      Crustaceans - mortality.
S=Sediment     P= Pimophalaa promotes D= Daphnia pufetx            (_) No Significant Difference   Fish - mortality, teratogenicity, or abnormal swimming behaviour.
YPF - average number of young per female - applicable only to   Cariodaphnia dubia.
Weight - applicable only to fish larval survival and growth test
                                                                       23

-------
Station Number Waterbody Description
Date of Collection
Continued...
07-Aug-91
13-Aug-91
05-Nov-91
OS-Nov-91
ODEQ9
Sample Test
Type Species
Test
Percent
Effected
Tributary of San Bots Creek
W P 20
S C 100
WC 100
W P
100
Control
Percent Significant
Effected Effects
10 Q
0 ®
Test Control Significant Test
Test Control Significant Weight Weight Dtfln Duration
YPF YPF DrfinYPF (mg) (mg) Weight (Days)
7
1
1
2
pH
(S.U.)
6.4
6.5
6.0
6.0
Tributary of Eliza Creek
Agency EPA Reach Latitude Longitude County State
Alkalinity Hardness
(mg/L) (mg/L)
32
143
21
21
ODEQ
12
104
38
38
Conductivity
(umhosfcm)
156
367
105
105
Total
Chlorine
(mg/L)
0.1
< 0.1
< 0.1
< 0.1
Total
Ammonla-M
0.1
4.3
1.3
1.3
V&AVtT QCtCtfOC"
OO nj 0 1 09 99 O9
Unionized
Ammonla-N
(mg/1)


OK
24-Mar-92
24-Mar-92
24-Mar-92
24-Mar-92
20-May-92
2(Hvtay-92
25-Aug-92
25-Aug-92
08-Sep-92
08-Sep-92
21-Oct-92
21-Oct-92
W
W
S
S
W
W
W
W
S
S
W
W
C
P
C
P
C
P
C
P
C
P
C
P
100
100
100
13
100
15
100
7
100
17
100
16
0 ®
7 (•)
0 ®
7 Q
0 ®
7 Q
0 ®
3 Q
0 ®
3 Q
0 ®
3 O
1
7
1
7
3
7
1
7
1
7
1
7
8.0
8.0
7.2
7.2
7.4
7.4
7.4
7.4
7.4
7.4
7.3
7.3
306
306
238
238
310
310
422
422
170
170
162
162
443
443
213
213
404
404
443
443
240
240
207
207
1170
1170
630
630
960
960
903
903
642
642
614
614
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
0.9
0.9
0.4
0.4
0.4
0.4
0.2
0.2
0.8
0.8
0.4
0.4
TA615
                Mlngo Creek at Apache Street Bridge In Tutea
                                                                                                                                            INCOG
                                                                                                                                                                    36M1-31-  95'51'39"
                                                                                                                                                                                              Tutea
                                                                                                                                                                                                        OK
20-Mar-90
20-Mar-90
17-Sep-90
11-Dec-90
11-D0C-90
H-Dec-90
11-Dec-90
0214.0160
04-Nov-92
04-Nov-92
08-Jun-93
08-Jun-93
0304.0100
25-Jan-93
Sample Types
U/-U/*htr
W C
W P
W C
W C
W P
S C
S P
10
7
0
0
0
0
7
o Q
10 Q
10 Q
10 Q
o Q
o Q
7 O
16.4 17.7

17.3 14.1
21.6 19.4

16.2 17.6

O

8

0

7
7
7
7
7
7
7
7.9
7.9
7.4
7.0
7.0
7.4
7.4
Wichita River at End ofEasttand Lane
W C
W P
W C
W P
0
0
20
3
10 Q
o Q
0 Q
3 O
8.9 16.3

19.1 18

®

O

7
7
7
7
7.9
7.9
7.3
7.3
Days Creek af Statellne Road South of Texattana
W C
Test Species
0

o O

21.8 19.2

o
Significant Effects
7

6.8

249
249
74
147
147
149
149
294 420
294 420
144 365
248 608
248 608
200 382
200 382
77VRCC 11130206001
204
204
188
188
1097 3710
1097 3710
956 433
956 433
TlfVftCC 11140302003
28

41 144

< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
ss-sffis" gs^e-so-
< 0.1
< 0.1
0.2
0.2
33*22"15" 94*02'33"
< 0.1

0.5
0.5
0.4
0.2
0.2
1.0
1.0
Wichita TX
0.5
0.5
1.2
1.2
Bowie TX
1.08

                                                                                    Fish - mortality, teratogenicity, or abnonnal swimming behaviour.
                                                                                                                                          Weight - applicable only to fish larval survival and growth test
                                                                                                                                                                                                          24

-------
Station Number  Waterbody Description
  Agency  EPA Reach    Latitude  Longitude     County    State
Sample Test
Date of Collection Type Species
Continued..
25-Jan-93
25-Jan-93
25-Jan-93
06-Jul-93
06-Jul-93
06-Jul-93
06-Jul-93
0404.0020
03-Dec-91
03-Dec-91
. 11-Feb-92
11-Feb-92
21-Mar-92
21-Mar-92
04-Aug-92
04-Aug-92
12-Jan-93
12-Jan-93
12-Jan-93
12-Jan-93
28-Jun-93
28-Jun-93
28-Jun-93
25-Jun-93
0505.0300
09-Jan-90
09-Jan-90
27-Mar-90
27-Mar-90
09-Aug-90
16-Od-90
16-Oct-90
04-Feb-91
24-Apr-91
Sampl* Types
Test
Percent
Effected
Control
Percent significant
Effected Effects
Test Control Significant Test
Test Control Significant Weight Weight Difln Duration
YPF YPF DlflnYPF (mg) (mg) Weight (Days)
PH
(S.U.)
Total
Alkalinity Hardness Conductivity Chlorine
(mg/L) (mg/L) (umhosfcm) (mg/L)
Total Unionized
Amntonla-N Ammonla-N •
(man.) (ma/i)
Days Creek af Statellne Road South of Texarkana 	 >
W P
S C
S P
W C
W P
S C
S P
0
0
10
0
3
60
100
3 O
o Q
3 Q
0 Q
0 Q
0 @
10 (•)

21.4 19.2

20.9 20

1.2 19.1


O

o

®

7
7
7
7
7
7
7
6.8
6.7
6.7
7.2
7.2
6.9
6.9
Big Cypress Crook at US 259
W C
W P
W C
W P
W C
W P
W C
W P
W C
W P
S C
S P
W C
W P
S C
S P
0
10
0
7
0
10
0
3
10
7
10
0
0
7
0
0
0 Q
3 Q
o Q
10 Q
o Q
7 Q
o Q
10 Q
o Q
7 Q
o Q
3 Q
o Q
o Q
o Q
0 Q
17.4 16.7

17.1 17.5

21 16.8

18.2 17.4

18.9 20.1

19.7 20.7

22.1 20

22.3 20

o

o

o

o

0

o

o

o

7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
6.8
6.8
6.4
6.4
6.8
6.8
7.1
7.1
6.6
6.6
6.8
6.8
6.7
6.7
6.6
6.6
Sabtne River at AT&SF Railroad Southeast of Longvtew
W C
W P
W C
W P
W C
W C
W P
W C
W C
Test Species
0
3
0
10
0
0
7
0
30

0 Q
7 Q
10 Q
7 Q
o Q
o Q
10 Q
10 Q
10 Q

17.3 16.2

15.2 14.7

18.2 18.7
19.1 18

18 15.5
13.3 16.7

o

0

8



Significant Effects
7
7
7
7
7
7
7
7
7

7.1
7.1
6.8
6.8
7.3
6.7
6.7
7.1
6.7

28
171
171
34
34
200
200
TNRCC
21
21
23
23
22
22
26
26
19
19
216
216
25
25
137
137
TNRCC
55
55
37
37
36
33
33
64
35

41 144
165 375
165 375
91 480
91 480
151 519
151 519
11140305004
40 173
40 173
47 186
47 186
41 146
41 146
45 150
45 150
33 120
33 120
239 438
239 438
52 194
52 194
163 374
163 374
12010002021
53 256
53 256
41 159
41 159
53 263
34 191
34 191
40 179
48 74

< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
32'54'18" 94'
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
« 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
1.08
2.4
2.4
0.3
0.3
19.2
19.2
43'25" Morris TX
0.5
0.5
0.4
0.4
0.4
0.4
0.4
0.4
0.36
0.36
4.08
4.08
0.5
0.5
1.9
1.9
32*24'56" 94*3ff12" Gregg TX
« 0.1
< 0.1
« 0.1
< 0.1
< 0.1
« 0.1
< 0.1
< 0.1
< 0.1

« 0.1
< 0.1
0.4
0.4
< 0.1
0.4
0.4
0.5
0.5

W-Water      C= Coriodaphni* dubi*   S= Cyprlnodon iwiagwfu*    (*) Significant Difference      Crustaceans - mortality.
S=Sediment    P= Pimephulea promote D* Daphnit pubx          (_) No Significant Difference   Fish - mortality, teratogenicity, or abnormal swimming behaviour.
YPF - average number of young per female • applicable only to  Caribdaprin/e dubi*.
Weight - applicable only to fish larval survival and growth test
                                                                   25

-------
Station Number Waterbody Description
Teat Control Ttst Control Significant Test
Sample Test Percent Percent signMcant T«st Control Significant weight Wtlght Dlf In Duration pH
Date of Collection Type Species Effected Effected Effects YPF YPF DWInYPF (mg) (mg) Weight (Daym) (S.U.)
Continued...
24-Apr-91
07-Aug-91
07-Aug-91
0601.0800
13-Feb-90
13-Feb-90
14-May-90
27-NW-90
27-Nov-90
28-Fab-91
28-Feb-91
15-Apr-91
15-Apr-91
20-Aug-91
20-Aug-91
0611.0100
16-Jan-90
16-Jan-90
27-Mar-90
27-Mar-90
09-Aug-90
IO-Oct-90
11-Feb-91
30-Apr-91
30-Apr-91
28-Aug-91
0700.0200
05-Nov-91
05-Nov-91
06-Jan-92
06-Jan-92
23-Feb-93
23-Feb-93
Sample Types
W=Water
Agency EPA Reach Latitude Longitude County State
Total Total Unionized
Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-N
(mgA.) (man.) (umhos/cm) (mg/L) (mg/L) (mg/1)
Sablne River at AT ASF Railroad Southeast of Longvtew
W P
W C
W P
10 3 Q
10 10 Q
7 10 O
14.7 17.2

O

7
7
7
6.7
7.6
7.6
Heches River Bridge on IH 10 Near Beaumont
W C
W P
W C
W C
W P
W C
W P
W C
W P
W C
W P
10 10 Q
7 3 Q
0 0 Q
0 0 Q
0 0 Q
20 10 Q
3 0 Q
o o Q
15 7 Q
10 20 Q
17 7 O
15.9 17.9
19.7 15.2
19.5 16.7
19.1 18.1
17.8 16.4

7.3 14.5

o
8
0
o

(§)

7
7
7
7
7
7
7
7
7
7
7
7.0
7.0
6.6
6.2
6.2
6.1
6.1
6.3
6.3
6.3
6.3
Angelina River Bridge on US 59 North of LulUn
W C
W P
W C
W P
W C
W C
W C
W C
W P
W C
0 0 Q
7 3 Q
0 10 Q
3 7 Q
0 0 Q
0 0 Q
0 0 Q
0 10 Q
10 3 Q
0 0 O
17.5 18.4
16.5 14.7

16.9 18.7
18.8 16.5
16.5 15.1
15.3 15.2
16.1 14.4
o
o

o
o
Q
o
o
7
7
7
7
7
7
7
7
7
7
6.8
6.8
7.0
7.0
7.1
7.0
5.9
6.7
6.7
6.9
Alligator Bayou at SH 214 In Port Arthur
W C
W P
W C
W P
W C
W P
Test Species
C= Carfodaohnfe dublm
100 0 ®
100 o ®
100 0 ®
100 3 W
o o Q
10 7 O
S= Cvorinodon variboafus (•) Sk



21.2 19.9

mificant Difference



o

Significant Effects
Crustaceans - mortality.
1
6
2
6
7
7

7.2
7.2
6.9
6.9
6.7
6.7

35
76
76
7WRCC
77
77
12
23
23
11
11
16
16
21
21
7NRCC
7
7
23
23
24
23
11
24
24
21
TNRCC
84
84
84
84
86
86
YPF -aver*
48
46
46
74
445
445
12020003003
119
119
40
140
140
20
20
24
24
92
92
362
362
106
1043
1043
85
85
106
106
108
108
12020005031
29
29
49
49
28
32
44
36
36
16
114
114
165
165
98
134
151
78
78
111
12040201010
143
143
79
79
121
121
Benumb
1680
1680
1135
1135
840
840
•rofvouna
< 0.1
< 0.1
< 0.1
ao'os-sg- 94*0574-
< 0.1
< 0.1
« 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
3-C2T2r 94M3-33"
< 0.1 <
< 0.1 <
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1 <
< 0.1
< 0.1
< 0.1
29452137" 93'58'40"
0.5
0.3
0.3
Orange 7X
0.7
0.7
1.1
0.7
0.7
0.2
0.2
0.7
0.7
0.5
0.5
Nacogdochet TX
0.1
0.1
0.7
0.7
0.6
0.5
0.5
0.2
0.2
0.4
Jefferson TX
< 0.1 10.0
< 0.1 10.0
< 0.1
< 0.1
< 0.1
< 0.1
oar female - anolicable onl
1.2
1.2
1.9
1.9
tvto Cfriodfohnif dubif
No Significant Difference    Fish - mortality, teratogenicity, or abnormal swimming behaviour.
                                                                                           Weight - applicable only to (Mi larval survival and growth test
                                                                                                                                                                        26

-------
Station Number  Waterbody Description
Agency  EPA Reach    Latitude Longitude     County   State
Test Control
Sample Test percent Percent Significant Test
Date of Collection Type Species Effected Effected Effects YPF
Continued...
23-Feb-93
23-Feb-93
19-Jul-93
19-Jul-93
19-Jul-93
19-Jul-93
03-Jan-94
03-Jan-94
03-Jan-94
03-Jan-94
0877.777?
13-Sep-90
0804.0400
13-Sep-90
0805.0050
27-Mar-90
27-Mar-90
31-Jul-90
31-Jul-90
10-Oct-90
22-Jan-91
09-Apr-91
09-Apr-91
0805.0100
13-Sep-90
0805.0300
13-Sep-90
01-Oct-91
01-Oct-91
21-Apr-92
21-Apr-92
Alligator Bayou at SH 214 In Port Arthur
S C 00
S P 73
WC 00
W P 73
S C 100 10
S P 100 3
W C 00
W P 10 3
S C 100 0
S P 100 3
Clear Fork Trinity River below Lake Benbrook
WC 0 10
Trinity River at US 79 Northeast of Oakwood
W C 0 10
Trinity River at FM 85 East ofEnnls
W C 0 10
W P 77
W C 0 10
W P 03
W C 00
W C 00
W C 10 10
W P 77
Trinity River at SH34 Southwest of Rosser
W C 0 10

Q 21'6
Q
Q 12.2
Q
(•)
@
Q 19.2
Q
(•)
(•)

O i6.s

O 16-i

O 16-3
Q
Q 20.2
Q
Q 18-6
Q 18.9
O 14-1
o

O 16.6
Test Control Significant Test
Control Significant Weight Weight Dtfln Duration pH
YPF DrflnYPF (mg) (mg) Weight (Days) (S.U.)

18.7

20.2



17.3




16.1

16.1

14.7

19.5

16.5
16.2
15.8


16.1

O 7
7
(§) 7
7
2
5
O 7
7
3
4

O 7

O 7

O 7
7
O 7
7
§7
7
7
7

0 7

7.4
7.4
8.2
8.2
7.4
7.4
6.8
6.8
6.9
6.9

7.4

7.3

7.8
7.8
7.7
7.7
7.6
6.8
7.6
7.6

7.6
Trinity River at South Loop SH 12 below Dallas
W C 10 10
W C 10 0
W P 37
W C 00
W P 10 7
Q 18-2
Q 17.4
o
Q 18.9
O
16.1
13.6

16.8

Sample Types Test Species
W=Water
S=Sediment

C= Ceriodaphnia dubia S= Cyprinodon vanooafu*
P= Pimaphabt pmmolaa r> Daphnia puhx

® Significant Difference
O No Significant Difference


8 i
7
O 7
7
Significant Effects
Crustaceans • mortality.
7.4
7.6
7.6
7.5
7.5

Total Total Unionized
Alkalinity Hardness Conductivity Chlorine Ammonla-N Amnupla-N
(mg/L) (mg/L) (umhos/em) (mg/L) (mgfl.) (ma/|)

217
217
110
110
187
187
32
32


TNRCC
142
TNRCC
86
TNRCC
127
127
119
119
88
131
124
124
TNRCC
108
TNRCC
105
118
118
122
122


133
133
161
161
140
140
104
104



721
721
1285
1285
808
808
1270
1270
927
927
12030102017
144
269

<
<
<
<
<
<





0.1 9.6
0.1 9.6
0.1 0.4
0.1 0.4
0.1 15.6
0.1 15.6
0.2 0.8
0.2 0.8


32*39140- 97'2ffS2"
<
12030201010 31'38'5-
144
435
12030105012
150
150
126
126
152
168
205
205
341
341
236
236
536
367
697
697
<
0.1 2.5
r 95*472r
0.1 1.0
32'18159- 96*21'33"
<
«


<
<
«
<
0.1 2.6
0.1 2.6
0.1 0.1
0.1 0.1
0.1 0.5
0.1 0.2
0.1 0.5
0.1 0.5
32'25-36- 95'2T45"
132
413
12030105016
140
164
164
180
180

456
640
640
366
366

<
0.1 1.0
32M2'27- 96'44'06"
<
<
<
<
<

0.1 0.7
0.1 0.7
0.1 0.7
0.1 0.2
0.1 0.2

YPF - average number of young per female - applicable only to
Fish - mortality, teratogenicity, or abnormal swimming behaviour.









.



Tarrant TX

Anderson TX

Navarro TX








Kaufman TX

Dallas TX





Ceriodaphnia dubia.
Weight - applicable only to fish larval survival and growth test





27

-------
Station Numb*- Watorkody Description
Agency  EPA Reach    Latitude  Longitude     County   State
Sample Test
Date of Collection Type Specie*
Continued^.
29-JUI-92
01-Sep-92
01-Sep-92
0805.0400
13-Sep-90
0806.0005
13-Sep-ao
0841.0500
29-Sep-92
29-Sep-92
29-Sap-92
29-Sep-92
11-Aug-93
11-Aug-93
H-Aug-93
11-Aug-93
06-Oec-93
06-Dec-93
06-Dec-93
06-Dec-93
0902.0100
12-Feb-90
12-Feb-90
11-Mar-90
11-Mar-90
13-Nov-90
28-Feb-91
28-Feb-91
06-May-91
06-May-91
15-Aug-91
15-Aug-91
Sample Type*
W'Water
S= Sediment
Te*t
Pcrcwtt
Effected
Control
Percent Significant
Effected Effect*
Test Control Significant Test
Test Control Significant Weight Weight Drfln Duration
YPF YPF DtflnYPF (mg) (mg) Weight (Days)
PH
(S.U.)
Total Total Unionized
Alkalinity Hardnen Conductivity Chlorine Ammonla-N Ammonla-N
(mgfl.) (mgfl.) (umhotfcm) (mgfl.) (mOfl.) (mgfl)
Trinity River at South Loop SH 12 below Dallas
W C
W C
W P
10
10
7
10 Q
0 O
3 O
16.6
11.2

18.5
15.3

0
(f)

7
7
7
7.5
7.1
7.1
Trinity River at Commerce Street In Dallas
W C
0
10 O
17.1
16.1
0
7
7.7
West Fork Trinity River at Beach Street In Fort Worth
W C
0
10 O
18.2
16.1
0
7
8.4
Lower West Fork Trinity River at BeHHne Road in Grand Prairie
W C
W P
S C
S P
W C
W P
S C
S P
W C
W P
S C
S P
0
3
0
0
0
3
0
0
0
0
0
3
o Q
o Q
o Q
o Q
o Q
0 Q
o Q
3 Q
o Q
3 Q
o Q
3 O
26.4

16.8

21.1

18.4

20.3

15.8

26.8

26.8

17.8

18.2

16.8

18.2

o

®

0

o

0

o

7
7
7
7
7
7
7
7
7
7
7
7
7.7
7.7
8.1
8.1
7.6
7.6
7.5
7.5
7.1
7.1
7.0
7.0
Cedar Bayou at US 90 Southwest of Dayton
W C
W P
W C
W P
W C
W C
W P
W C
W P
W C
W P
Te»t Specie*
O Csrfedapnnfe tfuMa
P= PfaMonaaw ammeau
0
3
0
7
0
100
7
20
3
10
43

0 Q
3 Q
o Q
10 Q
o Q
0 (•)
o Q
10 Q
10 Q
10 Q
7 ®

20.1

17.9

18.4


19.2

15.7


17.9

16

17.1


16.2

16.2


S= Cyprinodon venogeha (•) Significant Difference
D=Dao/ini.ou»M I) No SkmMeant Difference
o

o

o


o

o

Significant Effect*
Crustacean* - mortality.
Rmh . mortality, taratnoanicitv or ah
7
7
7
7
7
3
7
7
7
7
7

nnfm*il mvimmina
7.0
7.0
7.3
7.3
6.9
6.7
6.7
7.8
7.8
7.8
7.8

138 199
86 179
86 179
771 < 0.1
489 < 0.1
489 < 0.1
TNRCC 12030105022 32*4679* 96*4?18*
121 150
518 < 0.1
TNRCC 12030102007 32*45W 97*1771*
146 172
346 < 0.1
TNRCC 12030102004 32*45'46* 96*59'4r
154 191
154 191
186 154
186 154
141 182
141 182
143 128
143 128
127 192
127 192
188 217
188 217
780 < 0.1
780 < 0.1
494 < 0.1
494 < 0.1
746 02
746 02
470 < 0.1
470 < 0.1
477 < 0.1
477 « 0.1
586 < 0.1
586 < 0.1
7NRCC 12040203002 29*5870* 94*59'08*
77 119
77 119
166 258
166 258
74 106
90 100
90 100
158 194
158 194
122 158
122 158

362 < 0.1
362 < 0.1
593 < 0.1
593 < 0.1
298 < 0.1
314 < 0.1
314 < 0.1
468 < 0.1
468 < 0.1
558 < 0.1
558 < 0.1

0.4
0.5
0.5
Dallas TX
0.6
Tamnt TX
0.2
Dallas TX
02
02
0.1
0.1
0.6
0.6
02
02
0.5
0.5
0.6
0.6
Liberty TX
0.7
0.7
0.4
0.4
0.4
0.4
0.4
0.4
0.4
0.2
0.2

YPF « average number of young per female • applicable only to C*enxKiepnnM OUDM.
behaviour Wolnht . •nnlleahU onlv to fhh kuval uiivkial and nmuith hHt
                                                                                                                                                                            28

-------
Station Number
Date of Collection
1006.0200
28-Oct-91
28-Apr-92
28-Apr-92
26-Aug-92
1007.9200
14-Od-92
13-Jan-93
07-Apr-93
14-Jul-93
15-Oct-93
11-Jan-94
22-Apr-94
1113.0020
30-Sep-92
14-Dec-92
16-Mar-93
09-Jun-93
14-Sep-93
07-Dec-93
Waterbody Description
Sample Test
Type Species
Test
Percent
Effected
Control Test
Percent significant Test Control Significant weight
Effected Effects YPF YPF DrflnYPF (mg)
Control Significant Test
Weight Difln Duration
(mg) Weight (Days)
(S.U.)
Houston Ship Channel at Greens Bayou
W S
W C
W P
W S
0
10
7
23
3 Q
0 Q 15.6 22.3 (§)
7 O
9
7
7
9
7.7
6.8
6.8
7.4
Vlnce Bayou at Confluence with Houston Ship Channel
S S
W S
S S
W S
S S
W S
S S
100
10
100
0
13
7
17
7 (S)
3 Q
7 Q
3 Q
0 Q
7 O
6
9
6
9
9
9
9
7.4
6.8
6.8
7.1
7.1
7.2
7.2
Armand Bayou Tidal At Lower Mud Lake 1.4 Km Upstream of NASA 1 Bridge
W S
S S
W S
S S
W S
S S
17
10
3
23
0
10
3 Q
0 Q
7 Q
3 (•)
3 Q
3 O
8
9
9
9
9
9
8.6
7.3
7.3
7.3
7.2
7.6
Agency EPA Reach Latitude Longitude County State
Alkalinity
(mgfl.)
(mgfl.)
Conductivity
(umhos/cm)
Total
Chlorine
(mgfl.)
Total
Ammonla-N
(mgfl.)
TWRCC 12040104015 29'44'46" 95'10'Or
122
82
82
128
0
89
89
2000
12500
302
302
16500
TNRCC 12040104017 29
174
110
172
103
131
167
166
2000
128
1984
103



30300
460
17300
485
21300
7760
27500
< 0.1
< 0.1
< 0.1
< 0.1
•43-31- 95
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
1.0
1.2
1.2
0.4
•13'29"
12.0
3.8
25.2
2.0
1.4
9.6
15.6
TNRCC 12040204008 29'34127" 95*04'15"
114
93
89
112
99
143
2000
927
2869
1230
16240
30500
6820
24300
21800
24500
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
0.84
1.4
0.8
3.6
4.8
8.4
Unionized
Ammonla-N
(mgfl)
Harris TX


Harris TX

< 0.1




Harris TX
< 0.1



1200.7820
                  Bryan Municipal Lake near Dam Spillway
                                                                                                                                                     TNRCC
                                                                                                                                                                              30'38'24"   96'2V33-
                                                                                                                                                                                                         Brazos
                                                                                                                                                                                                                     TX
01-Oct-91
01-Oct-91
18-Feb-92
18-Feb-92
21-Jul-92
21-Jul-92
23-Feb-93
23-Feb-93
02-Aug-93
02-Aug-93
30-Nov-93
W
W
W
W
W
W
W
W
S
S
W
C
P
C
P
C
P
C
P
C
P
C
0
0
10
7
0
3
0
7
20
13
0
0 (
7 (
0 (
7 (
10 (
3 (
0 (
0 (
0 (j
7 (
0 (
~) 10.3
1
J 18
J
J 18.5
J
J 22.2
J
j) s
J
J 18.3
13.6

18.1

18.2

20.8

19

17.7
(§)

O

O

O

(§)

O
7
7
7
7
7
7
7
7
7
7
7
7.6
7.6
8.0
8.0
8.0
8.0
7.8
7.8
7.9
7.9
7.2
145
145
94
94
178
178
154
154
209
209
152
74
74
75
75
61
61
77
77
128
128
63
493
493
339
339
694
694
604
604
608
608
951
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
« 0.1
< 0.1
< 0.1
0.6
0.6
0.2
0.2
0.5
0.5
0.5
0.5
13.2
13.2
0.8
Sample Types  Test Species                                                               Significant Effects
W=Water       C= Coriodaphnifdubi*  S= Cyprinodon vmymtiu    (S) Significant Difference      Crustaceans - mortality.
S=Sediment    P= Pimephmlos promotes D= Daphnit pub*           Q No Significant Difference   Fish - mortality, teratogenicity, or abnormal swimming behaviour.
YPF • average number of young per female - applicable only to   Carfcdepnnfe dubla.
Weight • applicable only to fish larval survival and growth test
                                                                    29

-------
Station Number  Waterbody Description
  Agency  EPA Reach    Latitude  Longitude     County   State
                                       Test      Control                                         Test    Control  Significant     Test                                                 Total         Total        Unionized
                  Sample   Test       Percent     Percent   Significant    Test   Control  Significant Weight   Weight     Dtfln     Duration    pH     Alkalinity  Hardness  Conductivity    Chlorine    Ammonla-N    Ammonla-N
Date of Collection    Type Species     Effected     Effected     Effects     YPF    YPF   DHInYPF  (mg)     (mg)     Weight     (Days)    (S.U.)      (mgfl.)     (mg/L)   (umhos/cm)      (mg/L)        (mg/L)         (mgfl)
30-NOV-93
1200.7822
20-Mar-90
20-Mar-90
14-Aug-90
14-Aug-90
27-Nov-90
27-Nov-90
04-Feb-91
21-May-91
21-May-91
20-May-92
20-May-92
1200.7830
12-Feb-90
12-Feb-90
20-Mar-90
20-Mar-90
14-Aug-90
14-Aug-90
27-Nov-90
27-Nov-90
08-Jul-91
08-Jul-91
01-0ct-91
01-Od-91
18-Feb-92
18-Feb-92
21-Jul-92
21-Jul-92
23-Feb-93
23-Feb-93
02-Aug-93
02-Aug-93
Sample Types
tA/-U/a*Ar
W
P
7
3
u


7
7.2
Bryan Municipal Lake Malnbody
W
W
W
W
W
W
W
W
W
W
W
C
P
c
P
c
P
c
c
P
c
P
10
0
0
3
0
5
0
0
0
0
3
0
0
0
3
0
0
10
0
7
0
7
O 13.4
Q
Q 17.5
o
Q 19.4
(~)
Q 19.7
Q 17.5
Q
Q 21.2
o
17.7

17.2

16.7

15.5
14.8

16.2

(§)

O

o

8

O

7
7
7
7
7
7
7
7
7
7
7
8.1
8.1
9.3
9.3
8.3
8.3
5.9
8.9
8.9
7.7
7.7
Flnfeathor Lake near Dam Spillway
W
W
W
W
W
W
W
W
W
W
W
W
W
W
W
W
W
W
S
S
Test Species
f~s rmrif*t**h~
c
P
c
P
c
P
c
P
c
P
c
P
c
P
c
P
c
P
c
P
Im rtuttim
100
27
10
3
50
0
0
5
0
3
10
15
0
13
60
7
0
10
100
17

0
0
0
0
0
3
0
0
0
3
0
7
0
7
10
3
0
0
0
7
_r 	 *.._
©
(•)
Q 18.5
Q
Q 5.5
Q
Q 18.2
Q
Q 16.1
(™)
Q 18.3
Q
Q 16.3
(~)
(•) 16.2
(J
Q 21.3
o
X
o
nBn cinntfiMn* ruw.


17.7

17.2

16.7

16.3

13.6

18.1

18.2

20.8






o

o

o

0

o

o

o

o



Significant Effect*
fVi tftmf^mnm . nvufalifu
2
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
1
7

8.1
8.1
7.9
7.9
9.2
9.2
8.3
8.3
8.6
8.6
8.2
8.2
8.5
8.5
8.2
8.2
7.9
7.9
8.3
8.3

152
TNKCC
223
223
193
193
189
189
19
146
146
150
150
TNRCC
326
326
315
315
262
262
141
141
221
221
189
189
154
154
146
146
144
144
239
239
VDC _ etuAMj
63 951
< 0.1
0.8
30'38'2r 96*21'3r Brazos 7X
80 444
80 444
65 476
65 476
76 737
76 737
48 179
68 535
68 535
61 488
61 488
12070103034
78 952
78 952
65 776
65 776
74 857
74 857
88 1273
88 1273
75 901
75 901
77 818
77 818
71 551
71 551
41 655
41 655
101 662
101 662
194 688
194 688

< 0.1
< 0.1
< 0.1
< 0.1
« 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
SO'SSW 96*2
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
« 0.1
< 0.1
< 0.1
< 0.1
1
1

1.2
1.2
0.2
0.2
0.5
0.5
0.5
0.6
0.6
0.6
0.6
2-16- Braros TX
0.6
0.6
1.2
1.2
0.2
0.2
0.6
0.6
0.4
0.4
0.7
0.7
0.4
0.4
0.7
0.7
0.6
0.6
14.4
14.4
*L_ *»lu«H /"Vu^J...*!*!^ jJllJkW
                                                             (_) No Significant Difference    Fish - mortality, teratogenicity, or abnormal swimming behaviour.
Weight • applicable only to ftth larval survival and growth test

                                                                   30

-------
Station Number Waterbody Description
  Agency  EPA Reach     Latitude  Longitude     County    State
Date of Collection
Continued...
30-Nov-93
30-Nov-93
1200.7832
21-May-91
21-May-91
20-May-92
20-May-92
1200.7834
Sample
Type
Test
Species
Test
Percent
Effected
Control
Percent
Effected
Test Control Significant Test
Significant Test Control Significant Weight Weight DH In Duration pH
Effects YPF YPF DlfinYPF (mg) (mg) Weight (Days) (S.U.)
Total Total Unionized
Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-N
(mg/L) (mgfl,) (umhos/cm) (mgfl.) (mg/L) (mgfl)
Flnfeather Lake near Dam Spillway '—*
W
W
C
P
0
0
0
3
819.4 17.7 O
.
7
7
7.0
7.0
FlnfeatherLake Main Body
W
W
W
W
C
P
C
P
100
3
20
10
0
7
0
7
®
Q
Q 20.5 16.2 O
Q
4
7
7
7
8.2
8.2
8.0
8.0
Flnfeather Lake Headwater
154 117
154 117
687 < 0.1
687 < 0.1
7WRCC 12070103034 30*39'01" 96'2Z20T
198 76
198 76
144 82
144 82
837 < 0.1
837 « 0.1
470 < 0.1
470 < 0.1
TWRCC 12070103034 SO'SffH" 96'22'23"
0.4
0.4
Brazos
0.5
0.5
0.6
0.6
Brazos


TX




TX
    04-Feb-91
                    W
                                       100
                                                                                                                                    8.0
                                                                                                                                                64
                                                                                                                                                          56
                                                                                                                                                                    174
                                                                                                                                                                                  0.1
                                                                                                                                                                                              0.5
1218.0270 Nolan Creek 200 Yards Above Bell County WCID #1 Outfall
2S-Feb-90 W C
26-Feb-90 W P
27-Mar-90 W C
27-Mar-90 W P
04-Jun-90 W C
04-Jun-90 W P
07-Aug-90 W C
24-Sep-90 W C
24-Sep-90 W P
12-Feb-91 W C
28-May-91 W C
28-May-91 W P
15-Jul-91 W C
15-Jul-91 W P
0
0
0
3
40
13
0
60
10
0
10
0
0
10
10 Q
0 Q
10 Q
7 Q
10 Q
7 Q
10 Q
10 ®
3 Q
0 Q
0 Q
0 Q
o Q
7 O
15.2
15.8
5.5

17.3
0
15.9
11.5
14
14.1 O
14.7 O
14.2 ®

15.5 Q
17.2 ®
16.6 Q
13.2 O
15.9 O
7
7
7
7
7
7
7
7
7
7
7
7
7
7
8.1
8.1
8.0
8.0
8.0
8.0
7.9
7.8
7.8
8.1
7.8
7.8
7.4
7.4
1300.0710 Unnvtlte Bayou at CR S21, 230 Meters Upstream from Caney Creek Confluence
06-Apr-89 W C
06-Apr-89 W P
0
100
0 Q
0 ®
2.5
16.4 ®
7
7
8.5
8.5
1300.0717 Unnvtlte Bayou at CR 321 (Boons Road)
25-Aug-92 W C
25-Aug-92 W P
23-Sep-92 W P
Sample Types Test Species
0
40
3

o Q
3 ®
10 O

1.6

14.3 ®
Significant Effects
7
7
7

7.2
7.2
8.3

TNRCC
204
204
211
211
94
94
128
159
159
196
180
180
168
168
TNRCC
110
110
TNRCC
102
102
196

12070201062 31<06128" 97'42'12-
234
234
245
245
102
102
167
190
190
237
200
200
204
204
523
523
526
526
239
239
289
280
280
531
487
487
495
495
12090402006 21
273
273
3020
3020
< 0.1
< 0.1
< 0.1
< 0.1
« 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
}«5T08- 95M2'3r
< 0.1
« 0.1
12090402006
297
297
89

2500
2500
983

< 0.1
< 0.1
< 0.1

Be» TX
0.7
0.7
0.8
0.8
0.7
0.7
0.5
0.5
0.5
0.2
0.3
0.3
0.4
0.4
Matagorda TX
3.4 1.9
3.4 1.9
Matagorda TX
3.5
3.5
3.1

W=Water      O Coriodaphnla dubtt  S= Cyprinodon vtriegatus    (•) Significant Difference      Crustaceans - mortality.
S=Sediment    P= Pimephfba pmmelts D= Oipnnie pufex          O No Significant Difference    Fish - mortality, teratogenicity, or abnormal swimming behaviour.
YPF - average number of young per female - applicable only to  Cfriodtphnm dubit.
Weight - applicable only to fish larval survival and growth test
                                                                  31

-------
Station Number Watetbody Description
 Sample  TMt
  Type  Species
 Test
Percent
Effected
Date of Collection     Type  Species      Effected    Effected

Continued.-     Unnville Bayou at CK 321 (Boone Road)
Control                                         Tnt    Control  Significant    TMt
Percent   significant    Tnt   Control  Significant W*4ght   WalgM    Uf In     Duration    pH
           Effects     YPF    YPF   DM In YPF   (mg)     (mg)     WalgM     (Days)   (S.U.)
    Agency  EPA Reach    Latitude  Longitude     County   State

                                   Total        Total        Unionized
Alkalinity  Hardnan Conductivity   Chlorine    Amnwnla-N    Ammonla-N
 (tng/L)    (mgn.)  (umhos/cm)      (mg/L)       (mj/L)         (mg/l)
23-Sep-92
M-Oec-93
06-Dec-93
06-Dec-93
06-DOC-93
S
W
W
S
S
C
C
P
C
P
0
0
7
10
7
0 (
0 (
3 (
0 (
3 (
J 20.7
J 19.2
J
J 19.4
J
21.3
16.8

19.7

0
o

o

7
7
7
7
7
8.3
6.7
6.7
7.4
7.4
196
89
89
146
146
89
100
100
71
71
983
429
429
689
689
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
3.1
0.5
0.5
3.6
3.6
1300.0726
Unnvtlto Bayou at CR 324 (Haaoma Road)
                                                                                                TNRCC  12090402006   29TJ734-  9S*46"54>    Matagortta    TX
24-Apr-90
24-Apr-90
16-Jul-90
10-Oct-90
21-Jan-91
03-Apr-91
03-Apf-91
23-Jul-91
23-Jul-91
01-Od-91
01-Oct-91
02-Dec-91
02-Dec-91
24-Mar-92
24-Mar-92
07-Jul-92
07-Jul-92
25-Aug-92
25nAug-92
23-Sap-92
23-Sap-92
23-Sep-92
23-Sep-92
15-DBC-92
15-Oec-92
17-Mar-93
17-Mar-93
15-Jun-93
15-Jun-93
W
W
W
W
W
W
W
W
W
W
W
W
W
W
W
W
W
W
W
W
W
S
S
W
W
W
W
S
S
C
P
C
C
C
C
P
C
P
C
P
C
P
C
P
C
P
C
P
C
P
C
P
C
P
C
P
C
P
0
50
0
60
0
100
50
80
90
0
100
100
100
0
100
20
100
10
100
0
100
0
53
0
10
0
7
20
0
0 C
7 (J
0 C
o Q
o C
0 (
3 (
0 (
7 (
0 C
7 (
0 (
3 (
o C
7 (
10 (
7 (
o C
3 ti
o C
10 6
o C
10 ^
o C
3 C
0 £
7 £
o C
7 Q
~) 92
P
} 5.7
» o
} 16.6
;
;
) 5.5
5
J 4.9
5
;
5
} 0.4
5
) 3.3
5
J 2.3
?)
J 3.4
9
J 25.6
P
J 17.5
;
J 20.5
;
) 21.3
5
18.1 (•)

14.6 ®
16.5 ®
15.7 O


15.2 ®

13.6 (•)



17.6 (§)

13.1 (•)

14.3 (§)

21.3 (§)

21.3 O

18.7 O

19.7 O

18.3 O

7
7
7
7
7
1
7
7
7
7
5
1
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7.6
7.6
7.9
7.4
6.6
7.8
7.8
7.4
7.4
7.5
7.5
7.8
7.8
8.0
8.0
72
7.2
7.6
7.6
7.3
7.3
8.1
8.1
7.6
7.6
7.4
7.4
6.8
6.8
150
150
202
176
81
225
225
221
221
212
212
214
214
196
196
100
100
108
108
150
150
216
216
95
95
118
118
177
177
282
282
244
370
84
241
241
267
267
291
291
237
237
253
253
277
277
268
268
301
301
69
69
132
132
133
133
179
179
2240
2240
3520
3920
189
2270
2270
2340
2340
2040
2040
2550
2550
2400
2400
2660
2660
2300
2300
3420
3420
870
870
451
451
260
260
527
527
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
0.1
0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
6.0
6.0
12
12.0
0.5
18.0
16.0
15.6
15.6
7.7
7.7
7.7
7.7
13.0
13.0
1.8
1.8
4.8
4.8
0.4
0.4
5.3
5.3
0.2
0.2
0.5
0.5
0.2
0.2
Sampla Tvpn   Tnt Spacln

W=Water      C= Ceriodaphnim dubi*   S= Cyprinodcn vtritgttus

S=Sediment    P= HmapAafea prometoa D= Daphnlm puhx
            (•) Significant Difference
            O No Significant Difference
                                                                     Significant Effects

                                                                     Crustacean* - mortality.

                                                                     Fish - mortality, teratogenicity, or abnormal swimming behaviour.
  YPF - average number of young per female • applicable only to  Cariodmphnim dubi*.
  Weight - applicable only to fish larval survival and growth test

                                                                   32

-------
Station Number  Waterbody Description
                                                                                                                                                 Agency  EPA Reach    Latitude  Longitude     County    State
Test
Samplt Te»t Percent
Date of Collection Type Species Effected
Control
Percent Significant Test
Effected Effects YPF
Continued... LJnnvtlte Bayou at CR 324 (Hasema Road)
14-Sep-93 WC 0 0 Q
14-Sep-93 W P 7
06-Dec-93 W C 0
06-Dec-93 W P 7
06-Dec-93 S C 0
06-Dec-93 S P 10
• Q
i R
3 O
17.3
19.8
19

Test Control Significant Test
Control Significant Weight Weight Dtfln Duration pH
YPF DiflnYPF (mg) (ing) Weight (Days) (S.U.)
18.8
16.8
19.7

o
o
o

7
7
7
7
7
7
7.0
7.0
7.0
7.0
7.5
7.5
1300.0750 UnnvtOe Bayou at SH35, West of Old Ocean
25-Aug-92 W C 0
25-Aug-92 W P 3
23-Sep-92 S C 0
23-Sep-92 S P 3
06-Dec-93 W C 0
06-Dec-93 W P 3
06-Dec-93 S C 0
06-Dec-93 S P 7
o Q
3 Q
o Q
10 Q
0 Q
3 Q
0 Q
3 O
13.2
23.9
20.9
20.7

14.3
21.3
16.8
19.7

o
0
o
o

7
7
7
7
7
7
7
7
7.1
7.1
7.6
7.6
6.6
6.6
8.2
8.2
1302.0100 San Bernard River Bridge on FM 442 Northeast of Bollng
24-Apr-90 W C 0
24-Apr-90 W P 10
16-Jul-90 W C 0
10-Oct-90 W C 0
21-Jan-91 W C 0
03-Apr-91 W C 0
03-Apr-91 W P 3
23-Jul-91 W C 0
23-Jul-91 W P 20
0 Q
7 Q
0 Q
o Q
o Q
0 Q
3 Q
0 Q
7 O
18.9

10.4
17.7
20.1
20.2
19.6
18.1

14.6
16.5
15.7
17.3
15.2
o

(•)
Q
Q
o
0
7
7
7
7
7
7
7
7
7
7.5
7.5
8.4
7.8
6.3
7.7
7.7
6.7
6.7
1305.0075 Caney Creek at Bridge 200 Yards Below Unnvllle Bayou Confluence
06-Apr-89 W C 0
06-Apr-89 W P 7
24-Apr-90 W C 0
24-Apr-90 W P 3
16-Jul-90 W C 0
01-Oct-91 W C 10
01-0ct-91 W P 7
Sample Types Test Species
0 Q
o Q
0 Q
7 Q
0 Q
0 Q
7 O

17.2
19.3
12.3
15.1


16.4
18.1
14.6
13.6


o
o
8

Significant Effects
7
7
7
7
7
7
7

8.7
8.7
8.0
8.0
8.1
7.5
7.5

Total Total Unionized
Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-N
(mg/L) (mg/L) (umnosfcm) (mg/L) (mg/L) (mg/l)
160
160
114
114
127
127
7NRCC
130
130
204
204
124
124
116
116
TNRCC
67
67
144
177
28
225
225
90
90
TNRCC
219
219
144
144
148
174
174

300 3870
300 3870
171 1268
171 1268
108 698
108 698
12090402006
158 236
158 236
179 501
179 501
150 299
150 299
179 523
179 523
12090401002
84 167
84 167
232 884
268 805
40 77
227 689
227 689
142 378
142 378
12090402006
252 1605
252 1605
265 1268
265 1268
207 1091
238 994
238 994

0.1
0.1
< 0.1
< 0.1
< 0.1
< 0.1
29'04'10" 95M7-20"
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
29*1 ff4r K*SS3S'
< 0.1
< 0.1
< 0.1
< 0.1
« 0.1 <
< 0.1
< 0.1
< 0.1
< 0.1
28-5T08' 95M2'32"
< 0.1
< 0.1
< 0.1
< 0.1
« 0.1
< 0.1
< 0.1

,-*- .
1.0
1.0
0.6
0.6
1.9
1.9
Matagorda
0.4
0.4
1.3
1.3
0.2
0.2
1.1
1.1
Wharton
0.5
0.5
0.4
0.5
0.1
0.2
0.2
0.5
0.5
Matagorda
1.6 <
1.6 <
0.2
0.2
0.6
0.8
0.8




TX





TX







TX
0.1
0.1



W=Water      C= Coriodtphnit dubla  S= Cyprinodon varlegatm    (•) Significant Difference
S=Sediment    P= Pimaphalat promotes D= Dtphnia pulex          Q No Significant Difference
                                                                                       Crustaceans - mortality.
                                                                                       Fish - mortality, teratogenicity, or abnormal swimming behaviour.
YPF - average number of young per female • applicable only to  Ceriodaphnia dubla.
Weight • applicable only to fish larval survival and growth test
                                                                  33

-------
Station Number  Watertody Description
  Agency  EPA Reach    Latitude  Longitude     County    State
Sample Ttst
Date of Collection Type Species
Test
Percent
Effected
Control Test Control Significant Test
Percent significant Ttst Control Significant Wtlght Wtlght Off In Duration pH
Efftcttd Effects YPF YPF DtflnYPF (mg) (mg) Weight (Days) (S.U.)
Alkalinity Hardness
(mg/L) (mg/L)
Total Total Unionized
Conductivity Chlorine Ammonla-N Ammonla-N
(umhos/cm) (mg/L) (mg/L) (mg/l)
Continued., Caney Creek at Bridge 200 Yards Below Unnvllle Bayou Confluence
02-Dec-91 W C
02-Dec-91 W C
24-Mar-92 W C
24-Mar-92 W P
07-Jul-92 W C
07-Jul-92 W P
22-Sep-92 W C
22-Sep-92 W P
15-Oec-92 W C
15-Dec-92 W P
17-Mar-93 W C
17-Mar-93 W P
15-Jun-93 S C
15-Jun-93 S P
14-S«p-93 W C
14-Sep-93 W P
09-Dec-93 S C
09-Dec-93 S P
0
0
0
3
10
3
0
7
0
3
0
7
0
3
0
0
0
3
o O
3 Q
0 Q
7 Q
10 Q
7 Q
o Q
10 Q
0 Q
3 Q
o Q
7 Q
o Q
7 Q
0 Q
o Q
o Q
3 O
17.5 16.9

19.6 17.6

15.3 13.1

21 21.3

18.5 18.7

20.3 19.7

20.7 18.3

20.5 18.8

20.8 19.4

O

o

o

0

0

o

o

o

o

7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7.9
7.9
8.1
8.1
8.3
8.3
7.8
7.8
7.0
7.0
7.0
7.0
7.5
7.5
7.2
72
7.3
7.3
1305.0100 Caney Creek above Tidal at FM 52!
06-Apr-89 W C
06-Apr-89 W P
0
3
o Q
o O
18.6 16.4

o

7
7
8.8
8.8
1412.0140 Colorado River at SH163 In Colorado City
19-Apr-93 W C
19-Apr-93 W P
19-Apr-93 S C
19-Apr-93 S P
16-Aug-93 W C
15-Aug-93 W P
16-Aug-93 S C
16-Aug-93 S P
10
13
0
3
100
17
0
3
0 Q
3 Q
o Q
10 Q
0 ®
3 Q
0 Q
3 O
3 19.6

20.7 19.1



21 18.2

®

o



0

7
7
7
7
1
7
7
7
7.9
7.9
7.2
7.2
7.8
7.8
6.9
6.9
1421.0470 North Oincho River 400 Meters Downstream of Johnson Dam In San Angelo
15-0ct-91 W C
15-0ct-91 W P
Sample Types Test Species
100
0









Significant Effects
2
7

7.9
7.9

99
99
212
212
132
132
192
192
85
85
72
72
184
184
179
179
196
196
TNRCC
203
203
TNRCC
200
200
214
214
264
264
198
198
TNRCC
250
250

174
174
253
253
184
184
203
203
136
136
97
97
183
183
238
238
188
188
1191
1191
1090
1090
600
600
818
818
362
362
140
140
505
505
1114
1114
621
621
12090402006
189
189
529
529
12080002017
940
940
155
155
256
256
161
161

393
393

4420
4420
705
705
12370
12370
1155
1155

1428
1428

< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
« 0.1
< 0.1
< 0.1
< 0.1
< 0.1
02
02
02
02
28'56-26" 95'43118"
< 0.1
< 0.1
1.8
1.8
1.7
1.7
0.4
0.4
0.4
0.4
0.5
0.5
0.5
0.5
0.2
0.2
0.4
0.4
0.5
0.5
Matagorda TX
0.4 < 0.1
0.4 < 0.1
32'22-30- lOO'SrSO- Mitchell TX
< 0.1
« 0.1
< 0.1
< 0.1
0.2
0.2
< 0.1
< 0.1
3i'3i-2r ^oa•22•3tt
< 0.1
< 0.1

0.2
0.2
1.2
1.2
1.9
1.9
1.2
1.2
Tom Green TX
0.7
0.7

W=Water      C= Ceriodaphni* dubi»  S= Cyprinodon vtriogatus    (•) Significant Difference      Crustaceans - mortality.
S=Sediment    P= Pimephfha promotes D= Dtp/inn puhx           (_) No Significant Difference   Fish - mortality, teratogenicity. or abnormal swimming behaviour.
YPF - average number of young per female • applicable only to  Ceriodmphni* duA/a.
Weight - applicable only to fish larval survival and growth test
                                                                   34

-------
Station Number  Waterbody Description
                                                                                                                                                    Agency  EPA Reach    Latitude  Longitude     County    State
Sample
Date of Collection Type
Test
Test Percent
*p*cw» Efrecno
Control
Percent
Effected
Test Control Significant Test
Significant Test Control Significant Weight Weight Dtf In Duration pH
Effects YPF YPF DlflnYPF (mg) (mg) Weight (Days) (S.U.)
Total
Alkalinity Hardness Conductivity Chlorine
(mg/L) (mgfl.) (umhos/cm) (mg/L)
Total Unionized
Ammonla-N Ammonla-N
(mg/L) (mgfl)
Continued... North Concho River 400 Meters Downstream of Johnson Dam In San Angelo «...
29-Jan-92 W
29-Jan-92 W
21-Apr-92 W
21-Apr-92 W
28-Jul-92 W
19-Apr-93 W
19-Apr-93 W
19-Apr-93 S
19-Apr-93 S
16-Aug-93 W
16-Aug-93 W
16-Aug-93 S
16-Aug-93 S
C 0
P 3
C 0
P 13
C 0
C 0
P 10
C 0
P 0
C 0
P 7
C 0
S 0
10
0
0
7
0
0
3
0
10
0
3
0
3
Q 16.3 16.2
(~)
Q 20.6 16.8
Q
Q 18.2 17.7
Q 21.6 19.6
Q
Q 21.9 19.1
o
Q 18 17.5
o
Q 15.8 18.2
Q
O

o

8

O

0

®

7
7
7
7
7
7
7
7
7
7
7
7
7
7.9
7.9
7.1
7.1
8.2
8.1
8.1
7.1
7.1
8.2
8.2
7.5
7.5
1429.0800 Town Lake at Longhom Dam
04-Feb-91 W
04-Feb-91 W
05-Nov-91 W
05-Nov-91 W
04-May-92 W
04-May-92 W
25-Aug-92 W
25-Aug-92 W
C 0
P 7
C 0
P 0
C 40
P 0
C 0
P 13
0
10
0
3
0
3
0
3
O 15.9 15
(~j
Q 14.1 12
o
® 2.8 17.6
Q
Q 11-3 14.3
Q
0

o

®

®

7
7
7
7
7
7
7
7
7.6
7.6
8.2
8.2
8.1
8.1
7.6
7.6
1429.TMP1 Waller Creek at 2nd Street In Austin
04-Sep-91 W
04-Sep-91 W
C 0
P 15
0
0
0 7.1 14.7
o
®

7
7
7.4
7.4
1429.TMP2 Waller Creek @ 19th Street In Austin
04-Sep-91 W
04-Sep-91 W
C 0
P 5
0
0
8 " '"
o
7
7
7.8
7.8
1 429.TMP3 Waller Creek Q 38th Street In Austin
04-Sep-91 W
04-Sep-91 W
C 0
P 15
0
0
8 ™ 1"
o
7
7
7.7
7.7
143 213
143 213
100 204
100 204
246 467
300 631
300 631
250 194
250 194
205 384
205 384
245 231
245 231
613
613
482
482
1563
1549
1549
464
464
1260
1260
646
646
7A/RCC 12090205004
143 178
143 178
164 238
164 238
150 196
150 196
164 215
164 215
7NRCC
126 172
126 172
7NRCC
126 197
126 197
7NRCC
145 221
145 221
502
502
614
614
437
437
425
425

495
495

513
513

493
493
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
0.1
0.1
0.1
0.1
2.3
2.3
0.5
0.5
0.2
0.8
0.8
3.6
3.6
0.2
0.2
10.8
10.8
SO'lS'OO" 97*42'48"
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
« 0.1
« 0.1
< 0.1

« 0.1
< 0.1

< 0.1
< 0.1

< 0.1
< 0.1
0.4
0.4
0.4
0.4
0.5
0.5
0.5
0.5

0.7
0.7

0.9
0.9

0.9
0.9











"~
Travis TX








Travis TX
< 0.1
< 0.1
Travis TX

Travis TX

Sample Types  Test Species                                                               Significant Effects
W=Watec       C= Coriodaphnia dubia   S= Cyprinodon variogali/3     ® Significant Difference      Crustaceans - mortality.
S=Sediment     P= Pimephalea pmmolaa 0= Daphnia pub*           (_) No Significant Difference    Fish • mortality, teratogenicrty, or abnormal swimming behaviour.
                                                                                                                                                  YPF • average number of young per female - applicable only to  Ceriodaphnla dub/a.
                                                                                                                                                  Weight - applicable only to fish larval survival and growth test
                                                                                                                                                                                                                     35

-------
Station Number Waterbody Description
Agency  EPA Reach    Latitude Longitude    County   State
Test Control Test Control Significant Test
Sample Test Percent Percent Significant Test Control Significant Weight Weight Dtfln Duration pH Alkalinity Hardness Conductivity
Date of Collection Type Species Effected Effected Effects YPF YPF DtflnYPF (mg) (mg) Weight (Days) (S.O.) (mg/L) (mgfl.) (umhosfcm)
1803.0100
20-Jun-89
20-Jun-89
1803.0102
20-Jun-89
20-Jun-89
1803.0950
20-Jun-89
20-Jun-89
1906.0230
06-Jun-89
OS-Jun-89
11-Jun-89
11-D«c-91
11-Oec-91
24-Feb-92
24-Feb-92
12-May-92
12-May-92
07-Jul-92
07-Jul-92
OI-Dec-92
OI-Dec-92
01-Dec-92
01-Dec-92
17-May-93
17-May-93
17-May-93
17-May-93
12-Jul-93
12-Jul-93
12-Jul-93
Sample Types
WsWatsr

Guadalupe River at SH 175 South of Victoria
W D 3 7 Q
W P 17 7 O







2
8

8.0
8.0
Guadalupe River 0.5 Mile above SH 175 Bridge South of Victoria
W D 3 7 Q
W P 3 7 O


2
8
8.0
8.0
Guadalupe River 0.5 Mile below SH 175 South of Victoria
W D 10 7 Q
W P 13 7 O




2
8
8.0
8.0
Leon Creek at Ruiz Ranch Low Water Crossing. I.ISKm Downstream of Loop 13 South of San Antonio
WC 0 0 Q
W P 0 5 Q
S D 0 5 Q
W C 50 0 ®
W P 27 3 W
W C 0 0 Q
W P 15 7 Q
W C 0 10 Q
W P 7 3 Q
W C 10 10 Q
W P 7 7 Q
W C 0 10 Q
W P 37Q
S C 0 0 Q
S P 3 3 Q
w c o o Q
W P 73Q
S C -0 0 Q
S P 700
W C 000
W P 370
S C 0 10 0
Test Spades
21.6 20.7


10.2 19

13.5 15.9

12.4 19.1

14.1 13.1

17.1 17.4

21.6 18.7

21.6 18.7

21.5 20.3

21.5 19.1

21.9 18.4


P= Pimeohmlas ommolma D* Daonnfe Bute* O No Sianificant Difference
O


®

(§)

(§)

o

o

o

o

o

o

0
Sianificant Effect!
Crustaceans mortality
Fish • mortality, teretooenicitv. or abnormal n
7
7
2
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7

7.8
7.8
8.6
8.1
8.1
7.8
7.8
7.8
7.8
7.8
7.8
7.6
7.6
7.7
7.7
7.0
7.0
6.7
6.7
7.5
7.5
7.4

TNRCC
150
150
TNRCC
143
143
TNRCC
146
146
TNRCC
296
296

235
235
189
189
256
256
262
262
245
245
150
150
237
237
173
173
230
230
158

Total
Chlorine
(mgfl.)
Total Unionized
Ainntonla-N Amntonla^i
(mgfl.) (mgfl)
28M5-10- 97'0ff30- Victoria
148
148

148
148

148
148
342
342

328
328

313
313
< 0.1
< 0.1

< 0.1
* 0.1

< 0.1
< 0.1
12100302005 29-21-20" 98'
212
212

245
245
277
277
335
335
306
306
335
335
240
240
282
282
171
171
250
250
158

1002
1002
462
755
755
475
475
710
710
597
597
590
590
453
453
751
751
505
505
796
796
490

< 0.1
< 0.1

< 0.1
< 0.1
< 0.1
« 0.1
t 0.1
t 0.1
« 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1

0.8
0.8
Victoria
< 0.1
< 0.1
Victoria
0.7
0.7
'34-38- Bexar
1.2
1.2
3.6
4.8
4.8
22
2.2
0.3
0.3
0.4
0.4
0.4
0.4
1.5
1.5
0.2
0.2
1.3
1.3
1.2
1.2
1.2

TX


TX

TX


TX



0.3
0.3


















VDC _ •! !•«••• «inmL -. m* uuuui 	 * 	 »- --•ll..»lil» juttu 4>% f*~rin rim ntinlm tttJttm

M behaviour
i rr - «iwf«yv MMIIIUVI wi yvmiy pwi IWIIMIV • «pi/iiv««utv winy vw w*n iww«Bf. mow ww«.
                                                                                                                                                                                36

-------
Station Number Watertody Description
Test
Sample Test Percent
Date of Collection Type Specie* Effected
Control Test Control Significant Test
Percent significant Test Control Significant Weight Weight Off In Duration
Effected Effects YPF YPF DtfinYPF (mg) (mg) Weight (Days)
PH
(S.U.)
Agency EPA Reach Latitude Longitude County State
Total Total Unionized
Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-N
(man.) (man.) (umhosfcm) (mg/L) (mgA.) (mgA)
Continued.- Leon Creek at Ruiz Ranch Low Water Crossing. I.SSKm Downstream of Loop 13 South of San Antonio .,**. ,
12-Jul-93 S P
10-May-94 W C
10-May-94 W P
10-May-94 S C
10-May-94 S P
0
0
0
0
7
3 O
0 Q
3 O
0 Q
7 O

20.7 20.9

19.2 18.4


O

o

7
7
7
7
7
7.4
6.9
6.9
7.7
7.7
1906.0240 Leon Creek Approximately 25 Yards Below Kelly AFB Outfall 001
06-Jun-89 W C
06-Jun-89 W P
11-Jun-89 S D
40
IS
0
0 (•)
5 Q
s O
13.2 20.7


®


7
7
2
7.9
7.9
8.7
1906.0245 Leon Creek at Low Water Crossing Immediately Upstream of Kelly AFB Outfall 001
06-Jun-89 W C
06-Jun-89 W P
11-Jun-89 S D
0
10
0
0 Q
5 Q
5 O
21.2 20.7


0


7
7
2
8.1
8.1
8.2
1911.0300 San Antonio River at FM 1604 West of Elmendorf
28-Feb-90 W C
28-Feb-90 W P
05-Jun-90 W C
05-Jun-90 W P
31-Jul-90 W C
31-Jul-90 W P
29-Aug-90 W C
13-Nov-90 W C
19-Feb-91 W C
20-May-91 W C
20-May-91 W P
01-Dec-92 W C
01-Dec-92 W P
01-Dec-92 S C
01-Dec-92 S P
12-Jul-93 W C
12-Jul-93 W P
12-Jul-93 S C
12-Jul-93 S P
Sample Types Test Species
0
7
0
0
0
7
0
0
0
0
0
0
7
0
0
0
0
10
10

10 Q
0 Q
10 Q
7 Q
0 Q
3 Q
0 Q
0 Q
0 Q
o Q
7 Q
10 Q
7 Q
0 Q
3 Q
0 Q
7 (~)
10 Q
3 O

15.8 14.1

15.5 14.2

20.1 19.5

18.2 14.4
18.5 17.1
19.6 17.2
16.4 15.2

18.4 17.4

20.5 18.7

19.3 19.1

16.6 18.4


0

o

o

o
o
o
o

o

o

0

0

Significant Effects
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7

8.1
8.1
8.3
8.3
8.2
8.2
8.1
7.8
8.1
8.2
8.2
8.0
8.0
7.8
7.8
8.2
8.2
7.3
7.3

158
221
221
212
212
TNRCC
191
191

TNRCC
212
212

TNRCC
224
224
221
221
207
207
194
166
225
214
214
201
201
158
158
213
213
173
173

158
277
277
211
211
490
629
629
697
697
12100302005
284
284

1047
1047
496
12100302005
264
264

615
615
425
12100301010
240
240
286
286
313
313
296
264
313
284
284
302
302
169
169
290
290
178
178

843
843
873
873
901
901
592
552
637
844
844
719
719
435
435
883
883
528
528

< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
29*21'40" 98*34'59"
< 0.1
< 0.1

29'21'42" 98'34-58"
< 0.1
< 0.1

29'14'IS" 98'21'43"
< 0.1 «
< 0.1 <
0.1
0.1
0.1
0.1
< 0.1 <
0.2
< 0.1
« 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1

1.2
0.4
0.4
1.6
1.6
Bexw TX
3.4
3.4
3.6
Bexar 7X
0.7
0.7
2.4
flexar TX
0.1
0.1
0.7
0.7
0.3
0.3
0.1
0.5
0.5
0.2
0.2
0.6
0.6
0.2
0.2
0.4
0.4
1.2
1.2

W=Water      C= Ceriodtphnit dubit   S= Cyprinodon vtriegttut
S=Sedlment    P= Pf/mptietos pronetes D= Daphnit putox
(*) Significant Difference
O N° Significant Difference
Crustaceans - mortality.
Fish • mortality, teratogenicity, or abnormal swimming behaviour.
YPF - average number of young per female - applicable only to   Canbdap/m/a dubi*.
Weight - applicable only to fish larval survival and growth test
                                                                      37

-------
Station Humber Waterbody Description
  Agency  EPA Reach    Latitude  Longitude     County   State
Sample Test
Date of Collection Type Species
1911.0352
31-Jul-90
31-Jul-90
1911.0359
31-Jul-90
31-Jul-90
1911.0390
31-Jul-90
31-Jul-90
2202.0600
13-Feb-90
13-Feb-90
30-May-90
30-May-90
21-Jan-91
01-Apr-91
01-Apf-91
10-Jun-91
10-Jun-91
OI-Dec-93
01-Dec-93
01-Dec-93
01-Dec-93
2302.0025
21-Oct-92
21-Oct-92
25-Jan-93
25-Jan-93
20-Apr-93
20-Apr-93
21-Jul-93
Sample Types
Test
Percent
Efwcted
Control
Percent significant Test
Effected Effects YPF
Test Control Significant Test Total Total Unionized
Control Significant Weight Weight Dlf In Duration pH Alkalinity Hardness Conductivity Chlorine Ammonla-N Ainmonla-N
YPF DtflnYPF (mg) (mg) Weight (Days) (S.U.) (mgfl.) (mgfl.) (umnos/cm) (mgfl.) (mgfl.) (mgfl)
San Antonio River Above Confluence With Medina River
W C
W P
10
3
° P
3 O
18.8

19.5

O

7
7
8.2
8.2
San Antonio River Just Below Off/to Dam
W C
W P
10
7
0 Q
3 O
17.8

19.5

o

7
7
8.2
8.2
San Antonio River at River Bond Park, Near Southern Road
W C
W P
0
3
0 Q
3 O
19.4

19.5

o

7
7
8.1
8.1
Arroyo Colorado Mala F kxdway In Uano Grande at FM 1015 South of Weslaco
W C
W P
W C
W P
W C
W C
W P
W C
W P
W C
W P
S C
S P
100
3
10
10
10
0
10
80
7
20
3
0
7
0 ®
3 Q
20 Q
0 Q
0 Q
10 Q
7 Q
0 ©
3 Q
10 Q
3 Q
o Q
3 O


17.6

16.8
6.7

2.5

12.8

18.2



162

15.7
17.8

14

16.6

19.7



0




(§)

(§)

o

2
7
7
7
7
7
7
7
7
7
7
7
7
7.7
7.7
8.1
8.1
7.7
8.2
8.2
7.6
7.6
7.8
7.8
7.9
7.9
Rto Grande 0.3km Downstream from El Jardln Pumping Plant. 11.2km Downstream from Brownsvllle/Matamoros International Bridge (
W C
W P
W C
W P
S C
S P
S C
Test Species
10
3
0
7
0
7
10

10 Q
3 Q
0 Q
3 Q
0 Q
10 Q
10 O

17.1

20

22.4

20.4

16.8

19.2

19.1

18.4

o

0

o

o
Significant Effects
7
7
7
7
7
7
7

8.1
8.1
8.0
8.0
7.3
7.3
7.5

TNRCC
197
197
TNRCC
203
203
TNRCC
176
176
TNRCC
222
222
232
232
243
12100301011 29'14'OS- 98*24'30* Bexar
280 857 0.1 0.4
280 857 0.1 0.4
12100301011 29*16W 98'25'40* Bexar
276 812 0.1 0.4
276 812 0.1 0.4
12100302005 29*1715* 98*3ff45* Bexar
256 712 0.1 0.4
256 712 0.1 0.4
12110208014 26*0710* 97*5739" Hidalgo
886 3730 < 0.1 1.0
886 3730 < 0.1 1.0
500 3420 < 0.1 1.0
500 3420 < 0.1 1.0
728 2550 < 0.1 0.4
TX


TX


TX


TX





249 1000 4460 < 0.1 0.7
249 1000 4460 < 0.1 0.7
194
194
200
200
230
230
TNRCC
20
20
175
175
182
182
182

776 3470 < 0.1 1.3
776 3470 < 0.1 1.3
988 4230 < 0.1 0.7
988 4230 < 0.1 0.7
258 1101 < 0.1 13.2
258 1101 < 0.1 13.2
13090002001 25*52'35* 97*2715* Cameron
272 846 < 0.1 0.2
272 846 < 0.1 0.2
409 935 < 0.1 0.24
409 935 < 0.1 0.24
186 676 < 0.1 0.4
186 676 < 0.1 0.4
169 871 « 0.1 0.5







TX








W=Water      OC«riod»phnl»dubm   S= Cyprinodon variaoalu*    ») Significant Difference      Crustacean* - mortality.
S=S»dlmerrt    p= flhwpnato pro/nato 0= Dep/ime pufex           O f* Sflrafieant Difference   Fish - mortality, teratogenkaty, or abnormal swimming behaviour.
YPF-average number of young per female-applicable only to  CantodapAma dubi*.
Weight - applicable only to fish larval survival and growth test
                                                                 38

-------
Station Number  Watertody Description
  Agency  EPA Reach    Latitude  Longitude      County   State
Date of Collection
Sample
Type
Test
Species
Test
Percent
Entcted
Control Test Control Significant Test
Percent Significant Test Control Significant Weight Weight Dtfln Duration
Effected Effects YPF YPF Dtfln YPF (mg) (mg) Weight (Days)
PH
(S.U.)
Alkalinity
(mgfl.)
Hardness Conductivity
(mgfl.) (umhosrcm)
Total Total Unionized
Chlorine Ammonla-N Ammonla-N
(mgfl.) (mgfl.) (mgfl)
Continued.* Rio Grande 0.3km Downstream from El Jardin Pumping Plant, 11.2km Downstream from Brownsvllle/Matamoros International Bridge (US _^ r , .
21-Jul-93
21-Od-93
21-Oct-93
12-Apr-94
12-Apr-94
12-Apr-94
12-Apr-94
S
W
W
W
W
S
S
P
C
P
C
p
C
p
0
0
7
0
7
0
0
3 (
0 (
3 (
0 (
3 (
0 (
3 (
J
J 21.4 17.3 O

J 20.8 18.9 O
J
J 20.4 16.3 O
J
7
7
7
7
7
7
7
7.5
7.6
7.6
7.4
7.4
7.4
7.4
182
160
160
146
146
170
170
169
358
358
475
475
129
129
871
1638
1638
2070
2070
844
844
< 0.1
0.2
0.2
0.2
0.2
< 0.1
< 0.1
0.5
0.4
0.4
0.4
0.4
0.7
0.7
2302.0100
                 Rio Grande at International Bridge on US 77 In Brownsville
  TNRCC  13090002001   25*53'55"
                                                  Cameron
                                                                                                                                                                                                         TX
11-Dec-90
11-Dec-90
18-Nov-91
18-Nov-91
11-Mar-92
11-Mar-92
02-Jun-92
02-Jun-92
W
W
W
W
W
W
W
W
C
P
C
P
C
p
C
p
10
0
0
7
0
0
20
3
0 (
3 (
0 (
3 (
0 (
10 (
0 (
7 (
~) 17.8
J
J 12.3
J
J 18.5
J
J 14.8
J
19

10.3

16.9

15.3

O

0

0

O

7
7
7
7
7
7
7
7
8.2
8.2
8.3
8.3
7.7
7.7
8.1
8.1
300
300
216
216
127
127
118
118
137
137
427
427
260
260
249
249
1133
1133
1590
1590
795
795
1070
1070
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
1.2
1.2
0.7
0.7
1.7
1.7
0.5
0.5
2302.0150
                 Rio Grande below Anheto Drain South of Las Mllpas, at River km 244.1
  TNRCC   13090002002   26'03'47"  98<10*59>      Hidalgo     TX
21-Oct-92
21-Oct-92
25-Jan-93
25-Jan-93
20-Apr-93
20-Apr-93
21-Jul-93
21-Jul-93
20-Oct-93
20-Oct-93
12-Apr-94
12-Apr-94
12-Apr-94
12-Apr-94
2303.0100
19-May-93
Sample Types
W
W
W
W
S
S
S
S
W
W
W
W
S
S
C
P
C
P
C
p
C
p
C
p
C
p
C
p
0
0
0
7
0
0
0
3
0
0
0
0
0
0
o O
3 Q
0 Q
3 Q
0 Q
10 Q
10 Q
3 Q
0 Q
3 Q
o Q
3 Q
o Q
3 O
14.7 15.8

20.2 19.2

21.5 19.1

20.6 18.4

20.1 17.3

21.3 18.9

19.9 16.3

o

o

o

o

o

o

o

7
7
7
7
7
7
7
7
7
7
7
7
7
7
7.9
7.9
7.6
7.6
7.1
7.1
7.6
7.6
7.3
7.3
8.1
8.1
7.1
7.1
Falcon Lake at International Boundary Monument XIV
W
Test Species
C

0

0 O

21.2 18.7

o
Significant Effects
7

7.8

126
126
100
100
208
208
219
219
108
108
118
118
252
252
TNRCC
119

256 992
256 992
297 750
297 750
163 501
163 501
198 629
198 629
275 1371
275 1371
283 1150
283 1150
296 751
296 751

262 1020

< 0.1
< 0.1
•« 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
0.1
0.1
< 0.1
< 0.1
0.2
0.2
26'51'52" 9
< 0.1

0.6
0.6
1.2
1.2
1.8
1.8
0.4
0.4
< 0.1
< 0.1
0.8
0.8
1.3
1.3
9'18'2r Zapata TX
0.4

W=Water      C= Cariodtphnit dubit  S= Cyprinodon variagahu    ® Significant Difference      Crustaceans • mortality.
S=Sediment    P= Pimephmlaa promote D» Dtphnif potox           (j No Significant Difference   Fish - mortality, teratogenkity, or abnormal swimming behaviour.
YPF - average number of young per female - applicable only to   Carxxtopnn/e dubia.
Weight - applicable only to fish larval survival and growth test
                                                                39

-------
Station Number  Waterbody Description
                                                                                   Agency  EPA Reach    Latitude  Longitude     County   State
Sample
Oat* of Collection Type
Teat
Species
Twt Control
PWC^flt PWCWIt
Effected Effected
Significant Teat
Effects YPF
Twt Control Significant Test Total Total Unionized
Control Significant Weight Weight Dtfln Duration pH Alkalinity Hardnen Conductivity Chlorine Ammonla-N Ammonta-N
YPF NflnYPF (ing) (mg) Weight (Days) (S.U.) (mgfl.) (mgfl.) (umhoafcm) (mg/L) (mg/L) (mg/l)
Continued-. Falcon Ufa at totemafonalBounday Monument XV
19-May-93 W
17-Aug-93 W
17-Aug-93 W
P
C
P
3 3
0 0
0 3
0
0 16-7
O

17.5


O

2304.0075 Rio Grande at Pipeline Crossing, 13.2 km Downstream from Old Laredo/Nuevo Laredo International Bridge (US81),
23nAug-89 W
23-AUQ-89 W
18-Nov-91 W
18-Nov-91 W
11-D0C-91 W
H-Oeo-91 W
11-Mar-92 W
11-Mar-92 W
09-Jun-92 W
09-Jun-92 W
21-CW-92 W
21-Oct-92 W
26-Jan-93 W
26-Jan-93 W
12-Apr-93 S
12-Apr-93 S
06nAug-93 S
OS-Aug-93 S
28-Oct-93 W
28-Oct-93 W
14-Apr-94 W
14-Apr-94 W
14-Apr-94 S
14-Apr-94 S
C
P
C
P
C
P
C
P
C
P
C
P
C
P
C
P
C
P
C
P
C
P
C
P
0 0
0 7
10 0
0 3
0 0
7 3
10 0
7 10
0 0
10 3
0 0
7 3
0 0
3 3
0 0
3 3
100 0
7 0
0 0
7 3
0 0
0 3
0 0
7 3
O 19.6
Q
Q in
(J
Q 16.8
^)
O 17.6
^)
O n
o
Q 12.2
Q
Q 22.1
rj
Q 22.4
o
MM
Q
Q 17.2
Qj
Q 19-9
(J
Q 19.2
o
20.1

10.3

19

16.9

15.7

15.8

19.2

19.6



16.8

19.2

16.3

O

o

o

o

o

®

o

0



o

o

o

7
7
7
7.8
8.4
8.4
at River km 567.6
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
8.1
8.1
8.2
8.2
8.3
8.3
8.2
62
8.0
8.0
8.0
8.0
7.7
7.7
7.2
12
7.0
7.0
7.7
7.7
7.2
7.2
7.0
7.0
2304.0080 RbGrarte 9.0 Km Downstream of US 81 Bridge In Laredo
23-Aug-89 W
23-Aug-89 W
C
P
0 0
0 7
0 19-5
O
20.1

o

7
7
8.1
8.1
2304.0095 Rio Grande SO Yards Upstn3am from ConOuenoewUiZacata Creek
119
110
110
TNRCC
122
122
149
149
143
143
147
147
142
142
136
136
139
139
210
210
226
226
112
112
164
164
313
313
TNRCC
123
123
TNRCC
262 1020
264 1020
264 1020
13080002001
314 974
314 974
257 866
257 866
237 641
237 641
249 905
249 905
249 712
249 712
70 698
70 698
285 713
285 713
186 493
186 493
215 684
215 684
229 959
229 959
305 1310
305 1310
277 740
277 740
13080002001
310 967
310 967
13080002007
< 0.1
< 0.1
< 0.1
27-24'13" 99-29-13"
« 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
0.4
0.1
0.1
Webb TX
1.1
1.1
0.5
0.5
1.2
1.2
0.4
0.4
0.5
0.5
0.5
0.5
1.44
1.44
3.6
3.6
< 0.1 10.8
< 0.1 10.8
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
27*2ff21" 99*29'44-
< 0.1
< 0.1
27*29'55- 99*29'40>
0.5
0.5
0.5
0.5
8.4
8.4
Webb TX
1.2
12
Webb TX
    23-Aug-89
                   W
O
                                                                   20.4     20.1
o
                                                                                                                                 8.2
                                                                                                                                           121
                                                                                                                                                     312
                                                                                                                                                                959
                                                                                                                                                                          <  0.1
                                                                                                                                                                                         0.7
Sample TvBee  TeitSoeclei
W=Watar      O Ceribdepnnfc duNa  S= Cyprtnodon vanbgefua
SsSedlment    P= Hmapnafes promote D= OepAnfc putar
                          Significant Effect*
§   Significant Difference      Cmstaceen* - mortality.
   No SiQnincttn Diffwsoc*   Fish * rnortBlity, tornoQtnicjty, or •bnonruil swimming
                                                      YPF- average number of young per female, applicable only to  Ceribdapnnfe dublt.
                                                      Weight - applicable only to ftah huval tunlval and growth teat
                                                                                                                       40

-------
Station Number  Watertody Description
Agency EPA Reach   Latitude  Longitude    County   State
Sample Test
Date of Collection Type Species
Continued...
23-Aug-89
2304.0098
23-Aug-89
23-Aug-89
2304.0100
23-Aug-89
23-Aug-89
2304.0150
04-Feb-91
04-Feb-91
03-Deo-91
03-Dec-91
02-Mar-92
02-Mar-92
30-Jun-92
03-Nov-92
03-Nov-92
03-Nov-92
03-Nov-92
08-Mar-93
08-Mar-93
08-Mar-93
08-Mar-93
25-Oct-93
25-Oct-93
2304.0225
15-Dec-92
15-Dec-92
15-Dec-92
27-Oct-93
27-Oct-93
Sample Types
W=Water
SsSadirlMnt
Test
Pwcwit
Effected
Control Test Control Significant Test
Percent Significant Test Control Significant Weight Weight Dlf In Duration
Effected Effects YPF YPF Dlf In YPF (mg) (mg) Weight (Days)
Total Total Unionized
pH Alkalinity Hardness Conductivity Chlorine Ammonla-N Ammonla-N
(S.U.) (mg/L) (mg/L) (umhos/cm) (mg/L) (ma/L) (mg/l)
Rk) Grande 50 Yards Upstream from Confluence with Zacata Creek "" *" •'"
W P
0
7 O


7
8.2
Rto Grands 30 Meters Upstream of US 81 Bridge (Convent Ave) In Laredo
W C
W P
0
7
° fi
7 O
18.4

20.1 O

7
7
8.0
8.0
Rto Grands at Laredo Water Treatment Plant. 5.1km Upstream from Old Larodo/Nuevo Laredo International Bridge (US81). at River km
W C
W P
0
7
° P
7 O
18.5

20.1 O

7
7
8.3
8.3
Rto Grands 1 4 km Downstream from Eagle Pass/Pledras Negras International Bridge, near Irrigation Canal Lateral 50, at River Km 785.
W C
W P
W C
W P
W C
W P
W C
W C
W P
S C
S P
W -C
W P
S C
S P
W C
W P
0
7
0
10
0
3
10
10
7
0
0
10
0
0
3
10
0
0 Q
10 Q
o Q
3 Q
o Q
3 Q
0 Q
10 Q
3 Q
10 Q
3 Q
0 Q
3 Q
o Q
7 Q
o Q
3 O
13.7

18.8

16.3

15.4
12.1

20

22.2

20.9

14.7

15 O

16.7 O

16.2 O

13.5 Q
16.3 ®

17.4 O

18.7 O

19.7 O

17.3 ®

7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7.8
7.8
8.0
8.0
7.4
7.4
7.7
7.8
7.8
7.2
7.2
7.7
7.7
7.2
7.2
7.8
7.8
Rto Grands 4.5 Miles Downstream of Del Rto at Moody Ranch
W C
W P
S C
W C
W P
Test Species
C= Coriodaphnia dubia
Pa Pimoohaloa Dfomofaa
0
0
0
0
0
0 Q
3 Q
0 Q
o Q
3 O
19.2

13.2
19

18.7 O

15.2 Q
17.3 O

7
7
7
7
7
7.4
7.4
7.4
7.8
7.8
Significant Effects
S= Cyprinodon variogatua (W) Significant Difference Crustaceans - mortality.
D* Dao/ifiM 01
jlm ( INo
Significant C
Nfferanee Fi*h - mortality, teratooenlcitv. or abr
lormal swimminc
i behaviour.
121 312
959
TNRCC 13080002007
121 312
121 312
967
967
TNRCC 13080002007
125 316
125 316
983
983
TNRCC 13080002018
90 261
90 261
153 245
153 245
147 245
147 245
140 240
124 252
124 252
211 179
211 179
139 286
139 286
251 165
251 165
118 217
118 217
rNRCC
131 285
131 285
194 215
127 229
127 229
907
907
727
727
907
907
893
582
582
582
582
650
650
405
405
1068
1068

911
911
608
1002
1002
< 0.1 0.7
27'29-Sr 99*3076-
< 0.1 1.2
< 0.1 1.2
27*31'23- 99'31'26"
< 0.1 0.6
< 0.1 0.6
28'39'48- 100'30-Orr
< 0.5 0.5
< 0.5 0.5
< 0.1 0.5
< 0.1 0.5
< 0.1 0.2
< 0.1 0.2
< 0.1 0.4
< 0.1 0.4
< 0.1 0.4
2.5 2.5
2.5 2.5
< 0.1 0.2
< 0.1 0.2
< 0.1 3.6
< 0.1 3.6
0.1 0.5
0.1 0.5
29'17'31" 100*52'34"
< 0.1 0.1
< 0.1 0.1
< 0.1 1.92
0.1 0.5
0.1 0.5
YPF - average number of young per female - applicable only to
Weioht . .DDlieahle o
nlvtofishh
•rval sunHval and orawth test

Webb TX


Webb TX


Maverick TX

















Klnney TX





Coriodaphnlt dubia.

                                                                                                                                                                                    41

-------
Station Number  Watertody Description
                                                                                                                           Agency  EPA Reach    Latitude Longitude     County    State
Dflte of Collection
2304.0250
16-Mar-93
16-Mar-93
16-Mar-93
16-Mar-93
Sample
TVpt
TMt
Species
TMt
Percent
Effected
Control Test Control Significant Test
Percent significant Test Control Significant Weight Weight OK In Duration pH
Effected Effects YPF YPF DtflnYPF (mg) (ing) Weight (Days) (S.U.)
Rio Grande S.4km Downstream from Del Rto/Cludad Acuna International Bridge
W
W
S
s
C
P
C
P
0
0
0
0
0 (
7 (
0 (
3 C
3 22.2 19.7 O
J 20.6 19.4 O
7
7
7
7
8.0
8.0
7.6
7.6
Alkalinity
(mart.)
Hardness Conductivity
(mg/L) (umhosrcm)
Total
Chlorine
(mg/L)
Total Unionized
Ammonla-N Ammonla-N
(mgfl.) (mgfl)
77WRCC 13080001014 29'18'23" lOO'SS'OS' Val Verde TX
142
142
244
244
258
258
194
194
850
850
552
552
< 0.1
< 0.1
« 0.1
< 0.1
0.4
0.4
3.0
3.0
2306.0300
Rfo Grande below Rio Conchos Con/IUence, 14.4 km Downstream from Preskto/Ofnaga International Bridge, at River km 1,528.5
                                                                                                                                           TNRCC  13040201001   29'32W  104*21W    Presidio
                                                                                                                                                                                                       TX
30-Jan-92
30-Jan-92
20-May-92
20-May-92
21-Jul-92
21-Jul-92
20-Aug-92
20-Aug-92
09-Fab-93
09-Fab-93
11-Mar-93
11-Mar-93
14-Apr-93
14-Apr-93
15-Jun-93
15-Jun-93
25-Jan-94
25-Jan-94
25-Jan-94
25-Jan-94
W
W
W
W
W
W
W
W
S
S
W
W
s
s
W
W
W
W
s
s
C
P
C
P
C
P
C
P
C
P
C
P
C
P
C
P
C
P
C
P
0
7
10
7
0
0
0
7
0
3
0
7
0
0
0
30
0
7
0
0
10
7
0
7
10
3
0
0
0
7
0
3
0
3
0
3
0
3
0
3
O ".1
{*)
O 17.3
Q
Q 19.6
rj
Q 17.7
Qj
Q 222
(J
Q 20.6
{~)
Q 22.6
Q
Q 17.5
©
Q 20
(J
Q 22.3
O
16.2

16.2

18.2

15.7

19.9

18.7

19.6

19.3

21.4

21.1

O

0

O

O

O

O

O

O

0

O

7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
8.2
8.2
7.9
7.9
8.0
8.0
8.1
8.1
6.9
6.9
7.8
7.8
7.0
7.0
8.1
8.1
8.0
8.0
7.1
7.1
196
196
160
160
124
124
158
158
201
201
131
131
174
174
167
167
175
175
181
181
237
237
237
237
196
196
244
244
157
157
278
278
186
186
282
282
400
400
175
175
798
798
826
826
1022
1022
1084
1084
480
480
786
786
710
710
1103
1103
1978
1978
787
787
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
« 0.1
< 0.1
< 0.1
< 0.1
« 0.1
0.1
0.1
< 0.1
< 0.1
0.5
0.5
O.S
0.5
0.5
0.5
0.4
0.4
1.8
1.8
0.2
0.2
1.0
1.0
0.4
0.4
0.5
0.5
1.9
1.9
2308.0050
                Rfo Grande at Zaragosa International Bridge In El Paso, at River km 1,992.8
                                                                                                                                            TNRCC   13040100016  31'4ffOO"
                                                                                                                                                                           El Paso
                                                                                                                                                                                                        TX
14-Apr-92
14-Apr-92
01-Sep-92
01-Sep-92
23-Feb-93
23-Feb-93
W
W
W
W
W
W
C
P
C
P
C
P
10
10
40
13
0
0
0
3
0
3
0
0
Q 10.8
Q
® 12.8
Q
Q 20.1
O
18.2

15.3

20.8

®

O

O

7
7
7
7
7
7
8.0
8.0
7.7
7.7
7.4
7.4
184
184
164
164
181
181
253
253
256
256
298
298
184
184
870
870
1066
1066
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
< 0.1
3.0
3.0
0.9
0.9
1.9
1.9
Sample Types  Test Species                                                           Significant Effects
W=Water     C= Cwfadapnnfc duUa   S= Cyprinodon varfepahn    @ Significant Difference      Crustaceans - mortality.
S=Sediment   P= HmepAafea promote D= Dtphntm pufex           Q No Significant Difference   Fish • mortality, teratogenicity, or abnormal swimming behaviour.
                                                                                                                         YPF-average number of young per female-applicable only to  Cenbdep/infc due/a.
                                                                                                                         Weight - applicable only to fish larval survival and growth test
                                                                                                                                                                                        42

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Station Number  Watertoody Description
Agency  EPA Reach   Latitude  Longitude     County   State
Sample Test
Date of Collection Type Species
Continued...
22-Jun-93
22-Jun-93
22-Jun-93
22-Jun-93
20-Jul-93
20-Jul-93
20-Jul-93
20-Jul-93
28-Sep-93
28-Sep-93
28-Sep-93
28-Sep-93
2314.0100
22-Jun-93
22-Jun-93
28-Sep-93
28-Sep-93
28-Sep-93
28-Sep-93
2434.0100
30-Sep-92
10-Dec-92
11-Mar-93
18-Jun-93
09-Sep-93
07-Dec-93
2484.0200
23-Feb-93
23-Feb-93
23-Aug-93
23-Aug-93
Test Control Test Control Significant Test
Percent Percent Significant Test Control Significant Weight Weight Drfln Duration pH
Effected Effected Effects YPF YPF DrflnYPF (mg) (mg) Weight (Days) (S.U.)
Rto Grande at Zaragosa International Bridge In El Paso, at River km 1,992.8
WC 0 0 Q 21.9 18.6 O
W
S
S
W
W
S
S
W
W
S
S
P
C
P
C
P
C
P
C
P
C
P
7 3 (J
0 0 Q 20.3 20
7 0 Q
0 0 Q 21 20.2
3 3 Q
0 10 Q 19.9 18.4
3 3 Q
0 0 Q 19.1 16.3
7 0 Q
0 0 Q 17.7 19.9
0 3 O
O
O
O
O

®

7
7
7
7
7
7
7
7
7
7
7
7
7.7
7.7
8.0
8.0
7.7
7.7
7.4
7.4
7.8
7.8
7.7
7.7
Rio Grande at Courchesne Bridge (Hwy 273) In El Paso, 2. 7 km Upstream from American Dam, at River km 2, 020. 8
W
W
W
W
S
S
C
P
C
P
C
P
0 0 Q 21.7 18.6
03Q
0 0 Q 1" 16.3
7 0 Q
0 0 Q 15.8 18.2
3 3 O
O
O
O

7
7
7
7
7
7
8.4
8.4
8.3
8.3
7.6
7.6
Christmas Bay Midway Between Christmas Point and Rattlesnake Point
W
W
W
W
W
S
S
S
S
S
S
S
3 3 Q
7 0 Q
0 3 Q
0 7 Q
7 3 Q
0 3 O






8
9
9
9
9
9
7.7
7.7
7.9
7.6
8.2
7.4
Corpus Christl Inner Harbor near Navigation Boulevard Draw Bridge
W
S
W
S
S
S
S
S
0 3 Q
0 3 Q
17 7 Q
10 7 O
Sample Types Test Species
W=Water
S=Sediment

C= Canbdapnn/a dubia
P= nVnopnafos premeto


S= Cyprinodon vtriegttus (•) Significant Difference
D=0epnmapufex (_) No Significant Difference





Significant Effects
Crustaceans - mortality.
9
9
9
9


7.7
7.6
7.9
8.3


Fish - mortality, teratogenicity, or abnormal swimming behaviour.



Alkalinity Hardness
(mg/L) (mgfl.)
171
171
173
173
173
173
124
124
182
182
169
169
155
155
242
242
225
225
112
112
267
267
146
146
Total Total Unionized
Conductivity Chlorine Ammonla-N Ammonla-N
(umhos/cm) (mgn.) (mgfl.) (mg/l)
587
587
967
967
1027
1027
471
471
1230
1230
575
575
TORCC
182
182
194
194
228
228
258
258
296
296
146
146
993
993
1251
1251
954
954
TNRCC 12040205005
128
103
103
95
116
105

2000
2016
2996
2180

32800
34100
21200
28200
47700
25800
TNRCC
106
127
99
163

YPF-
2016
2016
2000
2000

37200
22400
41700
29600

0.2 0.1
0.2 0.1
< 0.1 1.6
< 0.1 1.6
0.1 1.8
0.1 1.8
< 0.1 3.6
< 0.1 3.6
0.2 0.4
0.2 0.4
0.1 1.4
0.1 1.4
31'48'10" 106'32'25"
< 0.1 1.2
< 0.1 1.2
< 0.1 0.1
< 0.1 0.1
< 0.1 9.6
< 0.1 9.6
29'03'30- 95MriO-
< 0.1 0.6
< 0.1 0.5
< 0.1 0.1
< 0.1 3.0
< 0.1 4.8
< 0.1 0.2
27*49'10" 97'2700"
< 0.1 0.4
< 0.1 3.6
0.2 3.6
0.1 15.6

average number of young per female - applicable only to








El Paso TX




Brazoria TX

< 0.1




AAieces TX
< 0.1
< 0.1



Coriodaphnia dubia.
Weight - applicable only to fish larval survival and growth test




43

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