TETRACHLORODIBENZO-P-DIOXINS AND -DIBENZOFURANS
IN EDIBLE FISH TISSUE AT SELECTED SITES
IN ARKANSAS, LOUISIANA AND TEXAS
MARCH 1990
TECHNICAL SECTION
WATER QUALITY MANAGEMENT BRANCH
U.S. ERA-REGION 6
1445 ROSS AVENUE
DALLAS, TX 75202-2733
ENVIRONMENTAL ANALYSIS SECTION
SURVEILLANCE BRANCH
U.S. EPA-REGION 6
1445 ROSS AVENUE
DALLAS, TX 75202-2733

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        UNITED  STATES  ENVIRONMENTAL  PROTECTION  AGENCY

                                     REGION 6
                              14*5 ROSS AVENUE. SUITE 1200
                                DALLAS, TEXAS 75202-2733
March 30, 1990

REPLY TO:  6W-QJ

MEMORANDUM
SUBJECT:

FROM:
Report on Dioxin in Fish Tissue
TO:
Philip Crocker
Technical Section (6W-OJ)
Water Quality Management Braa.cn

Carl Young
Environmental Analysis Sect>
Surveillance Branch

See Below
Attached for your  information and/or use is a  copy of the recently  completed
report "Tetrachlorodibenzo-p-dioxins  and  -Dibenzofurans in Edible Fish  Tissue
at Selected Sites in Arkansas, Louisiana and Texas."  This study involved follow-
up  monitoring  of  National  Bioaccumulation   Study  sites   where  2,3,7,8-
tetrachlorodihenzo-p-dioxin (2378-TCDD) was  found in edible fish tissue.  Seven
of the nine sites  investigated  are believed  to be a potential  long-term human
health concern  based  on  exceedances of the  EPA fish tissue criterion derived
using a 1  x 10   risk  level.   If you have any questions on the  report,  please
contact Philip Crocker at (FTS)  255-71457(214)  655-7145 or Carl  Young at
(FTS) 255-22897(214) 655-2289.

Attachment
Addressees:

Frank Shipley, GBNEP
Fred Kopflier, GMPO
Brian Cain, USFWS (Houston)
Tom Schultz, USFWS (Lafayette)
Steve Kroner, EPA HQ's (WH-553)
Tim Stuart, EPA HQ's (WH-553)
David Sabock, EPA HQ's (WH-585)
Rick Brandes, EPA HQ's (EN-336)
Renate Kimbrough EPA HQ's (A-101)
Mike Daggett (EPA-Houston, 6E-HO)
Harry Kreigh, ESAT (EPA-Houston, 6E-HO)
Steve Gonzales, USFDA (Dallas)
Sonya Delgado, USFDA (Washington, D.C.)
                                 Roger Meacham, 6X
                                 Ed Jeffrey, 6W-SP
                                 Leticia Lane, 6M-II
                                 George Pettigrew (ASTDR;6H-E)
                                 Russell Putt, 6W-QT
                                 Ken Teague, 6W-QT
                                 Craig Weeks, 6W-PI

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                                                              (_
TETRACHLORODIBENZO-P-DIOXINS AND -DIBENZOFURANS
     IN  EDIBLE  FISH TISSUE AT  SELECTED  SITES
        IN ARKANSAS,  LOUISIANA AND TEXAS
                Philip A.  Crocker

                Technical  Section
        Water Quality Management  Branch
 U.S. Environmental  Protection Agency-Region  6
                1445 Ross Avenue
             Dallas,  TX  75202-2733
                   Carl Young

         Environmental Analysis Section
               Surveillance Branch
 U.S. Environmental Protection Agency-Region  6
                1445 Ross Avenue
             Dallas, TX  75202-2733
                   March 1990

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                        EXECUTIVE SUMMARY

In 1986-87, as part of the U.S. Environmental Protection Agency's
(EPA) National Bioaccumulation Study (NBS),  samples of edible fish
tissue were collected and analyzed for 2,3,7,8-Tetrachlorodibenzo-
p-dioxin (2,3,7,8-TCDD) at 39 sites in Region 6.  Eleven sites in
Arkansas,  Louisiana and  Texas exceeded  0.7  parts  per trillion
(ppt) , the EPA criterion which applies to a risk level of 1 x 10"5
(1:100,000).   The  majority  of  these  waterbodies  were  located
downstream from bleached kraft pulp  and  paper mill discharges.  To
better appreciate possible human  health  risk associated with these
sites, EPA-Region 6  decided to collect additional data for 2,3,7,8-
TCDD in edible fish tissue,  for multiple species of fish such that
risk from consumption of these different species could be compared.

EPA-Region  6  requested  the  state's   assistance  in  collecting
samples. Chemical analysis for 2,3,7,8-TCDD, as well  as some other
important  congeners,  was  conducted  by  Triangle  Laboratories,
Research Triangle Park, North Carolina,  and the EPA Environmental
Research Laboratory,  Duluth, Minnesota.   The  Region  decided to
screen results against the EPA fish tissue criterion at a 1 x 10"4
(1:10,000)  risk level.  A fish consumption  rate of 6.5 g/day was
assumed, except for Louisiana, where a 20 g/day  was used, based on
application of this value in the state water  quality standards.
Risk  was evaluated  in terms of 2,3,7,8-TCDD, as  well  as  the
combined cummulative  risk of this and  other  congeners analyzed.
Exceedance of the 1  x 10"  risk level for a given species signified
to  the  Region  that  a  fish  consumption  advisory  should  be
considered, while exceedance of the 1 x 10"3 level would signify the
need for considering a fish consumption ban for that particular
species.  Exceedance of these levels in two or more species would
indicate that these recommendations  should apply to the fishery as
a whole. Where these risk levels are exceeded,  the state may wish
to  further  evaluate  risk  to  human   health   using  alternative
calculations of  risk, or  development  of policy  which addresses
allowable risk.

None of the sites sampled in this investigation exceeded the 1 x
10"3 risk level.  The following locations and species exceeded the
1 x  10"4 risk  level:  (1)  Red  River  at  Index,   blue  catfish;  (2)
Mississippi River below Zachary, channel catfish and white crappie;
(3)  Anacoco  Creek near  DeRidder,  channel  catfish;  (4)  Ouachita
River near the state  line,  channel  catfish  and carp; (5)  Houston
Ship  Channel   (tidal  San Jacinto River)  at  Morgans  Point,  sea
catfish  and  oysters;  (6) Houston  Ship Channel  at San  Jacinto
Monument, blue crab;  and  (7)  Neches River downstream of Evadale,
spotted gar.  The highest concentrations  of 2,3,7,8-TCDD were found
in fish  from the  Red River  (40.73 parts per trillion)  and crabs
from the Houston  Ship  Channel  (54.8 ppt).   These values approach
the 1 x 10"3 EPA risk level and exceed the FDA Level of Concern (25
ppt) . Other  sites not  sampled in the  present study,  but which
warrant concern are the Arkansas River below Pine Bluff, and Wham
Brake, Louisiana.

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These data serve to further reinforce  the  need for the states to
develop water quality criteria for 2,3,7,8-TCDD.
                             11

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                        Acknowledgements

EPA-Region 6 appreciates the assistance of the states in collecting
fish  samples for  this investigation,  most  notably John  Giese
(ADPCE); Kirk Manuel, Larry Racca,  Kirk Cormier,  M.  Hutchins, G.
C.  Bates,  Keith Casanova,  William Tucker  and Ronnie  Albritton
(LDEQ); and  Richard Seiler,  Linda  Broach,  Don Ottmers,  Suzanne
Meyers and Patrick Rogues (TWC).

We appreciate the assistance of Steve Kroner of EPA Headquarters,
and ERL-Duluth  in  arranging  for, and  analyzing six of  the fish
tissue samples,  respectively.

We greatly appreciate  the efforts of Harry Kreigh of  ESAT (EPA-
Houston Lab) for the quality assurance review of the data.

The chemical analyses were completed  by Triangle  Laboratories,
Research Triangle  Park, North Carolina.  Extramural  funding was
obtained through contract no. 68-03-3423, work assignment 2-24,
provided through the Assessment and Watershed Protection Division,
EPA Headquarters.  We particularly appreciate the help of Michael
McCarthy  of  Research  Triangle Institute  for facilitating this
contract.

We acknowledge the  review  of this  report made by David Neleigh, Ed
Jeffrey, Cheryl Overstreet and Jack Ferguson of EPA-Region 6, and
Carl Hickam, ATSDR.
                              111

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                           INTRODUCTION

Background

As part of the U.S.  Environmental Protection Agency  (EPA) National
Bioaccumulation Study (NBS), fish were collected from 47 sites in
Region 6 and analyzed in the late 1980's for chlorinated dibenzo-
p-dioxins and chlorinated dibenzofurans (CDDs and CDFs) dioxin and
furan  compounds,  as  well as  other bioaccumulative  pollutants.
Edible fish tissue samples were analyzed  at 39 of these sites, and
2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) was detected in
edible tissue at 13 sites.  With the exceptions of Bayou Meto and
Bayou DeLoutre, Arkansas,  and the tidal Brazos River near Freeport,
Texas, all of  these sites were located below  bleached kraft pulp
and paper mills.  This strongly suggests that bleached kraft pulp
and  paper mills  are  a  primary source  of  2,3,7,8-TCDD  in fish
tissue.

Except for Wham Brake in  Louisiana,  and  the Arkansas River below
Pine  Bluff,  samples of edible  tissue  were  analyzed on  only one
occassion.  Edible tissue from  11 sites were greater than or equal
to  0.7 parts  per  trillion  (ppt),  which  based on EPA's  risk
assessment approach, corresponds to a cancer  risk  level  of 1 x
10"5,  or one  chance  in  100,000  of  an individual developing cancer
(Table 1).  This assumption is based on an average consumption of
6.5  g/day over a 70-year period  of  exposure  (U.S. EPA 1980).
Edible tissue from two sites was greater  than 7 ppt (corresponding
to the 1  x  10"4 risk  level) ,  with  one  site  exceeding  the less
conservative  FDA  Level  of  Concern of  25  ppt.    EPA-Region  6
determined that additional data were  needed to resolve uncertainty
concerning health risks  associated with fish consumption at the
targeted sites.

Purpose and Objectives

The purpose of this  study was to determine if there is an elevated
risk  to human  health resulting from consumption  of fish due to
bioaccumulation of TCDDs and the related furans (TCDFs)  in edible
fish tissue downstream from discharges of bleached kraft pulp and
paper mills or other potential  point sources  of dioxin  in Region
6.   The  objective was to collect composite samples  of multiple
species of fish or other seafood organisms from NBS sites which had
not since  been  resampled, and where EPA-derived tissue criteria for
2,3,7,8-TCDD  equalled or exceeded a risk level of  1 x 10"5.  In the
case  of Bayou  DeLoutre,  Arkansas,  the tissue  concentration fell
slightly below this  level. However, both EPA Region 6 and the State
of Louisiana were  interested in evaluating this waterbody below the
state line, thus it was included.

Scope

Twenty-two fish or other  seafood samples  were collected at 9 sites

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TABLE 1
DIOXIN AND FURAN DATA FOR EDIBLE FISH TISSUE FROM SELECTED SITES IN ARKANSAS, LOUISIANA AND TEXAS:
NATIONAL BIOACCUMULATION STUDY SITES WHERE 2378-TCDD WAS DETECTED
CONCENTRATION IN PPT
SITE LOCATION
ARKANSAS
BAYOU HETO BELOW JACKSONVILLE
ARKANSAS RIVER BELOW PINE BLUFF
RED RIVER AT INDEX
BAYOU DE LOUTRE
LOUISIANA
WHAM BRAKE AT SWARTZ**
WHAM BRAKE AT SWARTZ**
BAYOU LAFOURCHE BELOW WHAM BRAKE**
MISSISSIPPI RIVER AT PORT ALLEN
BAYOU ANACOCO AT DERI ODER
OUACHITA RIVER AT MONROE
MISSISSIPPI RIVER AT ST. FRANCISVILLE
TEXAS
HOUSTON SHIP CHANNEL AT MORGANS POINT
LAKE SAM RAYBURN NEAR LUFKIN
NECHES RIVER TIDAL NEAR PORT ARTHUR
TIDAL BRAZOS RIVER AT FREEPORT
HOUSTON SHIP CHANNEL AT MORGANS POINT
LATITUDE

34 50 39
34 10 9
33 33 7
33 12 28

32 33 0
32 35 0
32 31 0
30 27 0
30 53 0
32 27 0
30 45 30

29 40 58
31 25 58
29 59 30
28 87 0
29 40 58
LONGITUDE

92
91
94
92

91
91
91
91
93
92
91

95
94
93
95
95

7 20
43 56
2 28
43 0

55 0
56 0
54 0
13 0
25 0
7 0
23 45

58 55
33 56
54 0
22 0
58 55
DATE

06-Mar-87
19-Nov-87
22-May-85
31-Dec-86

31-Jan-89
13-Feb-87
10-Dec-87
26-Mar-87
08-May-87
11-Feb-87
09-Aug-85

20-Mar-87
10-Dec-86
03-Mar-87
01-Jul-87
03-Jun-87
ORGANISM
ANALYZED (NO.)

BLACK CRAPPIE (1)
GRASS CARP (1)
SUCKER (1)
BASS (1)

CHANNEL CATFISH (3)
WHITE CRAPPIE (2)
BLUE CATFISH (5)
BIGMOUTH BUFFALO (3)
BLUEGILL SUNFISH (6)
LARGEMOUTH BASS (5)
LARGEMOUTH BASS (1)

OYSTERS (25)
WHITE BASS (4)
SHEEPSHEAD (4)
BLACK DRUM (6)
ATLANTIC CROAKER (9)
2378-
TCDD

23.1
8.85
1.69
0.62

48.16
14.95
5.18
1.83
1.4
1.03
0.82

6.7
0.94
0.69
0.66
ND
2378-
TCDF

12.41
69.82
5.23
ND

4.58
96.06
2.81
0.18
2.2
ND
0.85

14.13
0.8
0.31
14.77
1.38
TOTAL
TCDF

12.41
73.03
5.23
ND

4.58
96.06
2.81
0.18
2.2
ND
1.02

16.59
0.8
0.31
14.77
1.38
TEC*

24.36
16.25
2.61
1.59

50.25
25.55
5.99
2.59
1.63
1.05
0.94

8.12
1.08
0.76
13.59
0.19
RISK LEVEL
(X10-4)
2378-
TCDD

3.35
1.28
0.24
0.09

21.47
6.66
2.31
0.82
0.62
0.46
0.37

0.96
0.14
0.10
0.10
•
TEC*

3.53
2.35
0.38
0.23

22.4
11.39
2.67
1.15
0.72
0.47
0.42

1.18
0.16
0.11
1.97
0.03
*TEC=TOXICITY EQUIVALENCY CONCENTRATION
**RESULTS PRESENTED ARE THE MEAN OF DUPLICATE ANALYSES

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in the states of Arkansas,  Louisiana and Texas.  Edible tissue was
analyzed  for   2,3,7,8-TCDD,   total  TCDD,   2,3,7,8-Tetrachloro-
dibenzofuran (2,3,7,8-TCDF), and total TCDF.  Risks are evaluated
using EPA's risk assessment approach.

Bayou Meto,  the Arkansas River at  Pine Bluff,  Wham Brake, Bayou
Lafourche, and  the  tidal Brazos River were not resampled.  Wham
Brake has  been resampled, and additional sampling of  both Wham
Brake and Bayou Lafourche (located downstream  of  Wham  Brake)  is
being conducted by International Paper.  Bayou Meto is a Superfund
site, and contamination is being addressed under that program.  The
Arkansas  River  at  Pine  Bluff was  extensively resampled,  with
analyses contracted by International Paper.


                      METHODS AND MATERIALS

Site and Species Selection

Nine sites were selected for  collection and analysis of fish based
on data generated  from the NBS (Table 2)  .  Data from this study
showed elevated dioxin  levels  in  fish collected from these sites
(Table 1) .   Eight of  the  nine sites were located  downstream of
bleached kraft pulp and paper mills.  The  discharge of dioxin from
bleached kraft mills has been well documented (U.S.  EPA 1988).  The
exception was  Bayou  DeLoutre,  which was located  downstream  of
several possible pollution sources  including a PCB  incinerator
(ENSCO),  a wood treater (El Dorado Pole and Piling), and a chemical
manufacturing  plant  (Great  Lakes Chemicals).   It was  included
because dioxin was detected in a fillet sample  of a largemouth bass
analyzed during the NBS.

During the NBS,  some  fish samples  were  collected more  than ten
miles downstream of  the targeted  discharger.    For this study
sampling  crews  attempted to  collect  fish  two miles  or  less
downstream of targetted dischargers.  However, in some cases this
was not possible.

The  NBS  analyzed  only  one  fillet  composite  per  site.    It  is
difficult to make definitive statements  on risk with such limited
data.  For most sites edible tissue composites of three different
species were to be  collected for  analysis.  The target  number of
fish for each composite was five to six  individuals.  Each target
species was a popular game fish consumed by the public.

For freshwater sites the preferred fish  were:  one catfish species
(blue or  channel preferred),  one bass species,  one sunfish  or
crappie species, or other popular  game  fish  species.   From the
Houston Ship  Channel/Tidal  San Jacinto River it was decided  to
collect a composite of blue  crabs and a  composite  of oysters in
addition to fish.  Preferred species for the Houston Ship Channel
were croaker or  spot  and flounder.   However,  since these species
were not collected,  blue catfish from the San Jacinto Monument, and
sea catfish from Morgans Point were submitted for analysis.

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TABLE 2
SITES SELECTED FOR FOLLOW-UP ANALYSIS OF DIOXINS AND FURANS IN FISH TISSUE
SITE LOCATION
                                                                                                    UPSTREAM  DISCHARGES
                                                                     DATE           	
                                             LATITUDE    LONGITUDE   SAMPLED        NPDES #     FACILITY
                                                                                                                         CITY
ARKANSAS
RED RIVER AT INDEX
34 15  0    94  6  0     12-Sep-89     AR0002968   NEKOOSA-EDWARDS
                                      ASHDOWN,  AR
LOUISIANA

MISSISSIPPI RIVER BELOW ZACHARY

ANACOCO CREEK AT BOGGY CREEK ACCESS
OUACHITA RIVER NORTH OF STERLINGTON
BAYOU DE LOUTRE BELOW AR/LA STATE LINE
TEXAS
30 38 58    91 17 34     17-Aug-89

30 53 38    93 25  7     11-Aug-89
33  0  0    92  4  0     06-Sep-89
32 42 45    92 10 19     25-Aug-89
LA0005258
LA0003468
LA0007927
AR0001210
AR0037800
AR0001007
GEORGIA PACIFIC
JAMES RIVER CORP.
BOISE CASCADE
GEORGIA PACIFIC
ENSCO
GREAT LAKES CHEMICAL
ZACHARY, LA
ST. FRANCISVILLE, LA
DERIDDER, LA
CROSSETT, AR
EL DORADO, AR
EL DORADO, AR
                                                                                    AR0001171   EL DORADO POLE & PILING    EL  DORADO, AR
HOUSTON SHIP CHANNEL AT MORGANS POINT

HOUSTON SHIP CHANNEL AT SJ MONUMENT
LAKE SAM RAYBURN HEADWATERS
NECHES RIVER DOWNSTREAM OF EVADALE
29 45 13

29 44 19
31 26 3
30 6 57
95 5 39

95 7 11
94 34 5
94 4 31
03-Sep-89

28-Jul-89
31-Aug-89
30-Aug-89
TX0053023
TX0052591
n
TX0001643
TX0003891
CHAMPION INTERNATIONAL
GCWDA-WASHBURN TUNNEL**
n
CHAMPION INTERNATIONAL
TEMPLE-EASTEX
PASADENA, TX
HOUSTON, TX
n
LUFKIN, TX
EVADALE, TX
*BLUE CATFISH WERE ALSO COLLECTED  FROM THIS SITE ON 16-May-89
**FACILITY TREATS WASTEWATER  FROM  SIMPSON PAPER (TX0006041)

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 Sampling  Procedures

 Sample collection information is presented in Appendix 1.  Sampling
 was  conducted by state agencies at the  request of EPA Region 6.
 Instructions  were provided  to field personnel covering species
 selection,  processing and shipping of  fish samples.   Fish were
 collected using  electroshocking, gill nets, and hoop nets.  Field
 personnel were encouraged to  collect three composites  of fish,
 targeting five to six fresh  fish of one species and similar size
 per  composite.

 Once selected for analysis, individual fish were measured, weighed,
 wrapped in aluminum foil and taped together with other  fish in the
 composite.  A chain of custody  tag was completed and taped onto the
 composite.  Once  prepared the composite sample was refrigerated and
 frozen upon return to  the state office.

 Prior to shipping of samples, chain of custody forms were completed
 and  the   original  place  into  the  cooler being shipped.   Frozen
 samples were shipped on ice via overnight delivery to the  lab where
 they were to be analyzed.

 Chemical  Analysis

 Lab  analyses  were  performed  by Triangle  Laboratories,  Research
 Triangle   Park,   North  Carolina  (17   samples),   and  the  EPA
 Environmental  Research Laboratory,  Duluth,  Minnesota   (6 samples;
 sites included Red River at Index, Mississippi River at  Zachary and
 Ouachita  River north of Sterlington).

 Triangle  Laboratories  followed EPA Method 8290 with modifications
 in the internal  standards used (Triangle Labs  1989).   Procedures
 used by the EPA lab are documented in U.S. EPA-ERL  (1989).

 Fish were filleted at the  respective labs and the skin-off fillets
 were ground and homogenized.  A 20  gram aliquot  of the homogenized
 composite  was spiked with  an  internal  standard solution  and
 extracted in a Soxhlet extractor with a hexane/methlyene chloride
 solvent.    The extract is  then cleaned  up  using  chromatographic
 columns and concentrated prior to analysis by high resolution gas
 chromatography/high resolution mass spectrometry.

 Quality Assurance/Quality Control

 The  EPA  lab  followed  quality assurance/quality control (QA/QC)
 procedures  listed in  U.S.  EPA-ERL (1989),  while  Triangle  Labs
 followed QA/QC procedures  described in Triangle  Labs (1989).  Both
 labs  conducted  system performance   and calibration  checks  in
 addition  to  conducting duplicate  analyses of  selected  samples.
 Data were reviewed against  QA/QC criteria.   Data generated  by
Triangle  Labs was reviewed and found  to  be acceptable  by the EPA
Region 6  lab  in  Houston,  Texas.   This  review is presented  in
Appendix 2.

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Data Presentation

TCDD/TCDF data collected for the NBS and during the present study
were tabulated.   These tables include, sample location, organism
analyzed, number  of individuals constituting the composite, TCDD
and TCDF data, and risk level.  Overall risk waa estimated from the
concentrations of  2,3,7,8-TCDD as well as total risk, which would
include toxicity  of other TCDDs and TCDFs.   Samples analyzed by
ERL-Duluth  included  results  from  other  chlorinated dibenzo-p-
dioxins and dibenzofurans (CDD/CDF), and  these  values were also
taken into account in the calculation of  overall risk  (see below).

Estimation of Risk

Potential risk to  human health  from exposure to 2,3,7,8-TCDD was
calculated using the  following calculation:

RL= ql* x CR x Cone.
          BW

where:    RL=Risk  Level  (dimensionless)
          ql*=unit risk  (upper bound estimate of carcinogenic
              potency (mg/kg/day) "1
          CR=consumption rate (kg/day)
          Cone.=Concentration of contaminant in edible
              tissue  (mg/kg)
          BW=body weight of an average adult male (70 kg)

This approach  is  consistent with EPA  guidelines  and methodology
(U.S. EPA  1980;  1986; 1989a).   The unit risk is defined  as the
lifetime  risk due  to lifetime  exposure of  one  unit  of  cancer
concentration  expressed  as  (mg/kg/day)"1.    A  ql*  of  156,000
(mg/kg/day)"1  [B2]  was used in  developing the EPA  water quality
criteria  for protection  of human  health  (U.S.  EPA  1984).    A
standard consumption rate of 6.5 g/day  (0.0065 kg/day) was applied
for the states of Arkansas and Texas.   The U.S. EPA presently uses
this value in calculating water  quality criteria for protection of
human health.  Arkansas  and Texas have not yet promulgated human
health standards,  thus, the EPA consumption rate  was used as the
default value.    Louisiana has promulgated  human  health  water
quality standards which incorporate  a consumption rate of 20 g/day
(0.020 kg/day).  This value was  used for assessing Louisiana sites
in the present study.  The body weight value of 70  kg is  also a
standard assumption used by EPA and other agencies.   Fish tissue
concentrations of 0.07 parts per trillion (ppt) , 0.7  ppt,  and 7 ppt
2,3,7,8-TCDD equate to incremental cancer risks of 1 x 10"6, 10"5 and
10"4, respectively,  if a consumption rate of 6.5 g/day  is  used.
Using a consumption rate of 20 g/day, these criteria are 0.02, 0.2,
and 2 ppt, respectively.

The EPA risk-based approach  described  above is considered  to be
more appropriate  for defining  human health risk for particular
waterbodies than the U.S.  Food and Drug Administration (FDA)  Level

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of Concern  of  25 ppt.   First, the FDA value  is area-specific in
that it was developed for the Great Lakes region. The EPA approach
is applicable  nation-wide and can be modified to  reflect local
consumption patterns.  Second,  the FDA assumes only bottom feeding
species are contaminated and that they constitute only 10% of the
fish  consumed,  while EPA  assumes other types  of fish  are also
contaminated.   This assumption is supported  by NBS  fish tissue
data.  Third, FDA Action Levels and Levels of Concern relate to the
suitability  of seafood products  for interstate  commerce.   EPA
criteria  are  designed for  long-term protection of  consumers of
locally caught fish and shellfish. Data were assessed in terms of
2,3,7,8-TCDD alone, as well as  in  combination with other TCDDs and
TCDFs using  Toxicity Equivalence  Factors  (TEFs) as  described in
U.S.  EPA  (1989b).  TEFs are toxicity values for CDD/CDF compounds
relative  to 2,3,7,8-TCDD.   For example,  2,3,7,8-TCDD is the most
potent  CDD/CDF,  with a TEF value of 1.0,  while  2,3,7,8-TCDF is
considered  one-tenth as potent,   with a TEF  of 0.1.   To assess
overall risk, congener concentrations are multiplied by the TEFs,
and the products are then summed to obtain the total 2,3,7,8-TCDD
toxicity equivalency concentration (TEC) in the sample.  This value
represents the best  estimate of overall  risk  from consumption of
a particular species.

                     RESULTS AND  DISCUSSION

Approach

The data  generated in this  study  serve  to  provide  a better basis
for   decision-making  concerning  risk   associated  with   fish
consumption in Region 6 waterbodies receiving bleached kraft pulp
and paper mill discharges.  Fish tissue concentration results are
assessed using EPA-derived criteria in the following manner:

     -Where the  1  x 10"4  fish  tissue criterion is  exceeded,  the
state should consider issuing a fish consumption advisory for the
species of  concern, and  when it  is exceeded  by  more  than  one
species, an advisory for the entire fishery.

     -If  the 1  x 10"3  criterion is exceeded  the state  should
consider issuing a fish consumption ban for  that species, and when
it is  exceeded by more than  one species,  a  ban for the entire
fishery.

     -The state may establish other criteria, possibly through the
use of alternate risk estimation  procedures or policy to address
allowable risk in edible fish tissue.

The analytical  results  are evaluated  in terms of both 2,3,7,8-TCDD
concentrations and overall risk  (TECs).   Any alternative  risk
evaluation methods should be technically sound  and defensible.  The
state may also wish to conduct  additional sampling to further
delineate and verify areas of concern.  Where  the state concludes
there is significant risk to human health due  to consumption,  the
public should be informed.

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TABLE 3
DIOXIN AND FURAN DATA FOR EDIBLE FISH TISSUE FROM SELECTED SITES IN ARKANSAS, LOUISIANA AND TEXAS:
FOLLOW-UP SAMPLING OF SELECTED NATIONAL BIOACCUMULATION STUDY SITES
CONCENTRATION IN PPT
SITE LOCATION
ARKANSAS
RED RIVER AT INDEX
RED RIVER AT INDEX
LOUISIANA
MISSISSIPPI RIVER BELOW ZACHARY
MISSISSIPPI RIVER BELOW ZACHARY
MISSISSIPPI RIVER BELOW ZACHARY
ANACOCO CREEK AT BOGGY CREEK ACCESS
ANACOCO CREEK AT BOGGY CREEK ACCESS
ANACOCO CREEK AT BOGGY CREEK ACCESS
OUACHITA RIVER NORTH OF STERLINGTON
OUACHITA RIVER NORTH OF STERLINGTON
OUACHITA RIVER NORTH OF STERLINGTON
BAYOU DE LOUTRE BELOW AR/LA STATE LINE
BAYOU DE LOUTRE BELOW AR/LA STATE LINE
BAYOU DE LOUTRE BELOW AR/LA STATE LINE
TEXAS
HOUSTON SHIP CHANNEL AT MORGANS POINT
HOUSTON SHIP CHANNEL AT MORGANS POINT
HOUSTON SHIP CHANNEL AT SJ MONUMENT
HOUSTON SHIP CHANNEL AT SJ MONUMENT
LAKE SAM RAYBURN HEADWATERS
LAKE SAM RAYBURN HEADWATERS
LAKE SAM RAYBURN HEADWATERS
NECHES RIVER DOWNSTREAM BELOW EVADALE
NECHES RIVER DOWNSTREAM BELOW EVADALE
ORGANISM
ANALYZED (NO.)

BLUE CATFISH (6)**
FLATHEAD CATFISH (2)**

BLUE CATFISH (8)**
CHANNEL CATFISH (6)
WHITE CRAPPIE (4)
CHANNEL CATFISH (5)
LONGEAR SUNFISH (5)
STRIPED MULLET (5)
CHANNEL CATFISH (3)**
LARGEMOUTH BASS (3)**
CARP (3)**
LARGEMOUTH BASS (4)
BLACK BULLHEAD (3)
CARP (3)

SEA CATFISH (8)
OYSTERS (30)
BLUE CRAB (9)
BLUE CATFISH (3)
FRESHWATER DRUM (2)
CRAPPIE (2)
BLUEGILL SUNFISH (5)
LARGEMOUTH BASS (5)
SPOTTED GAR (5)
2378-
TCDD

40.73
2.26

1.4
6.6
1.5
7.4
1.5
1.8
3.14
ND
6.46
ND
ND
ND

14.8
6.1
54.8
3.2
ND
0.3
0.4
4.7
9.1
TOTAL
TCDD

40.73
2.26

1.4
6.6
1.5
7.4
1.5
1.8
3.14
ND
6.46
ND
ND
ND

14.8
13.2
66.2
3.2
ND
0.3
0.4
4.7
9.1
2378-
TCDF

10.98
ND

0.65
4.6
9.8
1.8
2.4
3.3
1.68
2.16
2.97
0.19
0.07
0.33

1.6
14.9
96.1
0.78
0.14
0.39
0.20
4.3
2.1
TOTAL
TCDF

10.98
ND

0.65
5.3
11.2
1.8
2.4
3.3
1.68
2.16
2.97
0.19
0.07
0.33

1.6
46.1
169.0
0.78
0.14
0.47
0.20
4.3
2.1
TEC*

42.36
2.26

1.47
7.06
2.48
7.58
1.74
2.13
3.33
0.22
6.78
0.02
<0.01
0.03

14.96
7.69
64.60
3.28
0.01
0.34
0.42
5.13
9.31
RISK LEVEL
(X10-4)
2378-
TCDD

5.90
0.33

0.62
2.94
0.67
3.30
0.67
0.80
1.4
-
2.88
-
-
-

2.14
0.88
7.93
0.46
-
.04
0.06
0.68
1.32
TEC*

6
0

0
3
1
3
0
0
1
0
3
0
-
0

2
1
9
0
-
0
0
0
1

.14
.33

.66
.15
.11
.38
.78
.94
.48
.10
.02
.01

.01

.17
.11
.36
.48

.05
.06
.74
.35
POSSIBLE
ADVISORY

YES
NO

NO
YES
YES
YES
NO
NO
YES
NO
YES
NO
NO
NO

YES
YES
YES
NO
NO
NO
NO
NO
YES
ACTIONS
BAN

NO
NO

NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO

NO
NO
NO
NO
NO
NO
NO
NO
NO
*TEC=TOXICITY EQUIVALENCE CONCENTRATION
**ALL SAMPLES DESIGNATED BY AN ASTERISK WERE ANALYZED BY ERL-DULUTH; 2378-TCDD WAS THE ONLY TCDD ANALYZED
                                                                                                                                                                  00

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Use of EPA's risk approach was a management decison based on best
professional judgement.   It does  not constitute formal Regional
policy.   It appeared to be  the most  viable means  by which to
address suitability of fish tissue containing TCDDs/TCDFs for human
consumption.  EPA used a risk level of 1 x 10"4 as the value above
which  the Region feels  some  action should be  considered by the
state.

Fish Tissue Results

Fish tissue  results are summarized by site in  Table 3, and are
discussed below.  Raw data  are  presented in Appendix 3.  The 1 x
10"  risk level criteria used  in evaluating data were  7 ppt for
Arkansas and Texas, and 2 ppt for Louisiana.

     Red River

Samples  were  collected just below  the Nekoosa-Edwards  discharge
canal.   The  blue  catfish  had  the second  highest  2,3,7,8-TCDD
concentration of the  sites  sampled in this study.   The  40.73 ppt
concentration equates to a risk level of  about 6 x 10~4'   This
concentration also  exceeds the  FDA 25 ppt Level of Concern.  The
six specimens making up the composite were relatively large, with
an average length and weight of 46 cm (18.5 in.) and 1.8 kg (3.9
lb.),   respectively.      Other  species   collected  which  had
concentrations below  the  risk  level of 1 x  10"4 include flathead
catfish,  and  a  sucker  (collected  for the  NBS).    Overall risk
associated with edible tissues  from these species  was 3.3 to 3.8
x 10"5.  A whole fish sample (smallmouth buffalo) was also analyzed.
This sample contained 9.01 ppt 2,3,7,8-TCDD.

     Mississippi River

Channel catfish and white crappie samples had TECs of  7.06 ppt and
2.48  ppt, respectively,  both  of  which  exceeded  the  l  x  10"4
criterion.  These  samples were  collected approximately one-half
mile downstream  of the  Georgia-Pacific discharge.    The channel
catfish was a composite of six fish which had an average  length and
weight of 35 cm  (14 in.) and 0.42  kg  (1 lb.), respectively.  The
crappie sample consisted of four specimens averaging 20  cm (8 in.)
in length and  0.1  kg  (0.23 lb.)  in weight.    Blue catfish also
collected at this site earlier in the year had   a  risk  of 6.6 x
10"5.   Bigmouth buffalo collected at Port Allen  for the NBS, also
had a relatively high  risk  (1.15  x  10"4) .  A largemouth bass sample
taken at St. Francisville, about four miles upstream of the Zachary
site during the NBS, had a risk level of 4.2 x 10"5.

     Anacoco Creek

A channel catfish  sample had a  tissue concentration of 7.4  ppt
2,3,7,8-TCDD,  which exceeded the 1 x 10"4 risk level.  This sample
was collected about 4  miles downstream of the Boise-Southern mill.
The composite sample consisted of five individuals with an average

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                                                                      10
length and weight of 30 cm  (12 in.) and 0.29 kg (0.6 lb.).  Other
species sampled which had  2,3,7,8-TCDD levels  below the 10"4 risk
level include longear sunfish, bluegill sunfish (collected during
the  NBS)  and striped mullet.  Risk levels for these  species ranged
from 7.3 to 9.4 x 10"5.

     Ouachita River

Channel catfish and carp samples had a tissue concentration of 3.14
ppt and 6.46 ppt 2,3,7,8-TCDD, respectively, which exceeded the 1
x  10**  risk level.  These  samples were collected just below the
Arkansas/Louisiana  state line,  about 16 miles  downstream of the
Georgia-Pacific  mill.  The  catfish  sample consisted of  three
individuals with an average length and weight of 33.5 cm (13.4 in.)
and 0.53 kg  (1.2  Ibs.), respectively.   The carp sample consisted
of three individuals averaging 54.3 cm (21.4 in.) in  length and 2.2
kg (4.9 Ibs.) in weight. The other species sampled was largemouth
bass (two  samples,  one  of which was  collected during NBS),  with
risk levels of 1.0 and 4.7 x 10"5.  It should be  noted that  the bass
sample collected during the  NBS  about 65 miles downstream of the
mill, had a tissue concentration of about 1 ppt 2,3,7,8-TCDD.

     Bayou DeLoutre

None  of  the samples  collected  during  the  present  study  had
detectable  2,3,7,8-TCDD,  thus risk level  was considered to  be
negligable.   Sampling was  conducted below the Arkansas/Louisiana
state line approximately 25  miles  downstream of El  Dorado,  where
several   industries   discharge,   including  a   hazardous  waste
incinerator.  This  was  the only case  in  the present study where
sampling  was  done   downstream   of  non-paper  mill  industrial
facilities.   A tissue  sample  from  a  single largemouth  bass
collected much farther upstream in El  Dorado, Arkansas, during the
NBS had a concentration of  0.62  ppt  2,3,7,8-TCDD and an overall
risk  of  2.3 x 10"5,  based  on the TEC.   Overall, this stream is
considered to be of relatively low concern.

     Houston Ship Channel/Tidal San Jacinto River

Two sites  were evaluated  in this  system  during the  study,  the
Houston Ship Channel at  about one mile upstream  of the San Jacinto
Monument in the vicinity of the state monitoring station 10060100,
and the tidal portion of the San Jacinto River at Morgans Point,
near state  monitoring  station  10050100.    The Monument  site  is
located approximately 5 miles downstream of the Champion mill and
10 miles below  the Gulf Coast Waste  Disposal  Authority Washburn
Tunnel discharge,  while  the Morgans Point site is located about 14
and 19 miles below these facilities, respectively.

At the Monument,  blue  crabs had a tissue concentration of 54.8 ppt
2,3,7,8-TCDD, while blue catfish had  much lower concentration at
3.2 ppt.   The crab concentration  was the highest  found  in  this
study.   TCDFs were also highly elevated in the crabs, in comparison
to the catfish.   The crab sample, consisted of a composite of nine

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                                                                      11
individuals with  a  mean carapace width of 12.3 cm  (5  in.)-   All
soft tissue was analyzed which  includes  the  edible flesh as well
as the hepatopancreas.  The hepatopancreas, or "mustard", which is
occasionally  consumed  by  humans,   generally  contains  higher
concentrations of toxicants than the associated flesh.   Inclusion
of  this  organ in  the analysis  could  have elevated the overall
concentration, although this may be appropriate considering it is
consumed by some persons.   The risk level associated with the crab
flesh was about 9  x 10"4'   The tissue concentration also exceeded
the FDA Level of  Concern.   Although  segment  1006  does  not have a
fishery use designated  in  the state  water quality standards (TWC
1988), based on state and EPA observations, there  is an actual use
of  crabbing.    Both  commercial crabbing  using  crab  pots,  and
recreation crabbing,  particularly  near the San Jacinto Monument,
has been frequently observed during warm weather months.

The  blue  catfish  is  a freshwater species which  enters tidally
influenced waters  during low salinity periods.   Salinity of the
Ship Channel at the time of sample collection  was unusually low.
Because this  is  considered to  be a  transient species  in  this
waterbody, its tissue concentration may underestimate health risk
associated with consumption of more indigenous species.   Based on
our observations,  we believe commercial or recreational harvest of
finfish in segment 1006 is relatively insignificant.

In terms of the Morgans Point site,  where  there is an  an aquatic
life  use  designated  in the  water  quality  standards,  both  sea
catfish and oysters were analyzed.  The concentration of 14.8 ppt
2,3,7,8-TCDD in sea catfish  equates  to a  risk  level of about 2 x
10"4.  The TEC of 7.69 ppt in oysters  equates to  a risk level of
about 1.1 x 10~4.  It be recognized, however,  that sea catfish are
rarely sought after and consumed by humans.  The concentrations of
2,3,7,8-TCDD and 2,3,7,8-TCDF in oysters found in the present study
agreed closely with those found  at  this site during the NBS, which
suggests that  tissue concentrations are  in  equilibrium  and are
stable over time.

These results show  that, based  on  the  the highly bioaccumulative
tendency of 2,3,7,8-TCDD, this compound has far-reaching influence
in the aquatic food chain  and results  in elevated concentrations
in seafood many miles downstream from  potentially responsible point
sources.

     Lake Sam Rayburn

None of the  three species analyzed  in this study (freshwater drum,
crappie,  and bluegill  sunfish) had fish tissue concentrations above
the 10"4 risk level.  Risk ranged from undefined to about 6 x 10"6.
A white bass sample collected during the  NBS had slightly higher
tissue concentration  (0.94 ppt), with  a risk level  of  1.6 x 10"5.
These samples were collected  in the lake headwaters about 2.5 miles
downstream of Papermill Creek confluence with the Angelina River.

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                                                                      12
     Neches River

A spotted gar sample had a tissue level of 9.1 ppt, which exceeded
the 10"4 risk level.  This composite sample, collected one-half mile
below the  Temple-Eastex discharge canal on the  east  side of the
river, consisted  of five individuals with an  average length and
weight of 48.6 cm (19 in.)  and 0.46 kg (1 lb.).  A largemouth bass
sample  had  a  2,3,7,8-TCDD  concentration  of  4.7 ppt,  with  an
associated risk of about 7 x 10~5.  Previous sampling during the NBS
found risk of 1.1 x  10~5 for sheepshead in the tidal portion of the
Neches  River,  approximately  50  miles  downstream  of the  mill.
Again, this demonstrates the far-reaching bioaccumulative effects
of dioxin on the  ecosystem, and in relation  to potential risk to
human health.

     Other Sites

Two other sites for which some data are available, but which were
not sampled in  this  investigation are the Arkansas River below Pine
Bluff and Wham Brake, Louisiana.  Risk levels for these two sites
were greater than 1  x 10~4 and 1 x 10"3, respectively,  based on NBS
data  (Table  1) ,  as  well as some  follow-up analyses  conducted by
EPA.  The states are presently concerned with these sites and are
working with the paper mills to collect additional data.

Relevance of Water Quality Standards for 2f3.7.8-TCDD

The data,  which  show  exceedances  of  EPA  fish  tissue  criteria,
demonstrate  the  need   for  the  states to  adopt  water  quality
standards for 2,3,7,8-TCDD.  Such standards would be the mechanism
for water quality-based toxics control of dioxin  in pulp and paper
mill  effluents.   The  assessments  of  risk  also  demonstrate the
utility  for  use of chemical  specific numeric criteria  for fish
tissue.

                          CONCLUSIONS

Concentrations  of  2,3,7,8-TCDD,  and/or TECs  in  edible  tissue
exceeded EPA  criteria  at the 10   risk  level for the  following
locations and species:  (1) Red  River  at Index,  blue  catfish; (2)
Mississippi River below Zachary, channel catfish and white crappie;
(3) Anacoco  Creek near  DeRidder,  channel  catfish;  (4)  Ouachita
River near the state line, channel catfish and carp;  (5)  Houston
Ship  Channel  (tidal San Jacinto  River)  at  Morgans Point,  sea
catfish  and  oysters;  (6)  Houston  Ship Channel  at   San  Jacinto
Monument,  blue crab;  and  (7)  the Neches River downstream  of
Evadale, spotted gar.   Although not sampled during this study, the
Arkansas River below Pine Bluff and Wham Brake, Louisiana are also
a concern with regard to human health.  Due to the relatively low
TCDD/TCDF concentrations found  at  Bayou DeLoutre,  Louisiana, and
Lake  Sam Rayburn,  Texas,  these  sites appear to be of  lesser
concern.

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                                                                      13
Due to the relatively high  concentrations  of 2,3,7,8-TCDD found,
the Red River  and Houston Ship Channel are  of  greatest concern.
From some multiple location sampling within given waterbodies, it
is evident that  the bioaccumulative effects  of  2,3,7,8-TCDD are
far-reaching,  as elevated  tissue  levels were  found many  miles
downstream of the potentially responsible point sources.


                         RECOMMENDATIONS

It is recommended that the states consider issuing fish consumption
advisories for water  bodies where fish tissue  concentrations of
2,3,7,8-TCDD alone or in combination with congeners, exceed a risk
level of  1  x 10"4.   The states may  wish to  evaluate risk  using
alternative methods and/or establish a policy by which to address
contaminants in  edible  fish tissue.   It is  recommended that the
states develop water quality standards for 2,3,7,8-TCDD to ensure
adequate water quality-based control of bleached kraft paper mill
discharges.

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                                                                       14
                         LITERATURE CITED


Triangle  Laboratories.    1989.     Triangle  Laboratories.  Inc..
Polvchlorinated Dibenzodioxins and Dibenzofurans. Data User Manual.
87 pp.

TWC.  1989.  Texas  Surface Water Quality Standards.   Texas Water
Commission, Austin, Texas.

U.S. EPA.  1980.   Water quality  criteria documents; availability.
Federal Register 45(231);79318-79379.

U.S. EPA.   1984.   Ambient Water  Quality Criteria  for 2.3.7.8-
Tetrachlorodibenzo-p-dioxin.  February 1984, EPA-440/5-84-007.

U.S. EPA.    1986.   Guidelines   for carcinogen  risk assessment.
Federal Register 51(185):33992-34003.

U.S. EPA.    1988.   U.S.  EPA/Paper Industry  Cooperative  Dioxin
Screening Study.  March  1988, EPA-440/1-88-025.

U.S. EPA.   1989a.  Assessing Human Health  Risks from Chemically
Contaminated Fish and Shellfish;  A Guidance Manual.   September
1989, EPA-503/8-89-002.

U.S.  EPA.    1989b.    Interim  Procedures  for  Estimating  Risks
Associated with  Exposures to Mixtures  of Chlorinated Dibenzo-p-
dioxins and -Dibenzofurans (CDDs and CDFs) and  1989 Update.  March
1989, EPA/625/3-89/016.

U.S. EPA-ERL.  1989.  Analytical Procedures and Quality Assurance
Plan  for the  Determination  of  PCDD/PCDF  in  Fish.    U.S.  EPA,
Environmental Research Laboratory,  Duluth, Minnesota.  April 1989.

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         Appendix 1
Sample Collection Information

-------
SITE NAME: Red River near Index, Arkansas immediately below
           Nekoosa-Edwards

COUNTY/PARRISH, STATE: Miller County, Arkansas

LATITUDE/LONGITUDE: 34 15    94 06

SITE DESCRIPTION: Red River just below Nekoosa-Edwards effluent
                  canal

COLLECTION METHOD: Trammel net

COLLECTORS: John Giese
            Arkansas Department of Pollution Control & Ecology

SAMPLING DATE: September 11-12, 1989

FISH COLLECTED:
   Sample #: DF025218
   Species: Smallmouth Buffalo  (Ictiobus bubalusl
   Length and Weight:
   1)  47 cm  1816 g
   2)  40 cm  1135 g
   3)  54 cm  2860 g
   4)  50 cm  1998 g
   5)  43 cm  1407 g
   6)  43 cm  1407 g

   Sample #: DF025219
   Species: Blue catfish (Ictalurus furcatus)
   Length and Weight:
   1)  50 cm  1226 g
   2)  43 cm  817 g
   3)  51 cm  1362 g
   4)  55 cm  1725 g
   5)  54 cm  1589 g
   6)  57 cm  1816 g

   Sample #: DF025220
   Species: Flathead catfish (Pylodictus olivaris)
   Length and Weight:
   1)  61 cm 2361 g
   2)  52 cm 1453 g

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SITE NAME: Mississippi River below Zachary, Louisiana

COUNTY/PARRISH, STATE: East Baton Rouge Parrish, Louisiana

LATITUDE/LONGITUDE: 30 39   91 17

SITE DESCRIPTION: Left descending bank on the Mississippi River.
                  Approximately 1/2 mile from paper mill outfall,
                  Color of water was black due to effluent from
                  mill.

COLLECTION METHOD: Electroshocking

COLLECTORS: K. Casanova, W. Tucker, R. Albritton,
            Louisiana Department of Environmental Quality


SAMPLING DATE: August 17, 1989

FISH COLLECTED:
   Sample #: DF025216
   Species: Channel Catfish (Ictalurus punctatus)
   Length and Weight:
   1)  40.6 cm  680 g
   2)  40.0 cm  737 g
   3)  35.6 cm  425 g
   4)  31.8 cm  227 g
   5)  32.4 cm  227 g
   6)  32.4 cm  227 g

   Sample #: DF025217
   Species: White Crappie (Pomoxis annularis)
   Length and Weight:
   1)  19.1 cm  85 g
   2)  22.9 cm  113 g
   3)  21.9 cm  142 g
   4)  18.1 cm  85 g

   Sample #: DF025007 (Episode 3418, Collected May 16, 1989)
   Species: Blue Catfish (Ictalurus furcatus)
   Length and Weight:
   1)  34.3 cm  396 g
   2)  33.0 cm  368 g
   3)  27.9 cm  198 g
   4)  29.2 cm  283 g
   5)  30.5 cm  283 g
   6)  26.7 cm  170 g
   7)  27.9 cm  198 g
   8)  27.9 cm  198 g

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SITE NAME: Anacoco Creek (Bayou) at Boggy Creek access below
           Boise Southern outfall

COUNTY/PARRISH, STATE: Vernon Parrish, Louisiana

LATITUDE/LONGITUDE: 30 53 38   91  25 07

SITE DESCRIPTION:  Anacoco Creek below Boise Southern outfall at
                   Boggy Creek access

COLLECTION METHOD: Hoop nets and wire traps

COLLECTORS: K. Manuel and L. Racca, Louisiana Department of
            Environmental Quality

SAMPLING DATE: August 11, 1989

FISH COLLECTED:

   Sample #: DF025213
   Species: Channel catfish (Ictalurus punctatus)
   Length and Weight:
   1)  30.0 cm  255.0 g
   2)  30.7 cm  237.5 g
   3)  21.0 cm  72.0 g
   4)  34.0 cm  399.0 g
   5)  36.2 cm  479.0 g

   Sample #: DF025214
   Species: Longear sunfish (Lepomis megalotis)
   Length and Weight:
   1)  14.8 cm  91.3 g
   2)  12.7 cm  44.8 g
   3)  14.0 cm  62.8 g
   4)  14.0 cm  65.0 g
   5)  13.4 cm  54.5 g

   Sample #: DF025215
   Species: Mullet (Mugil cephalus)
   Length and Weight:
   1)  25.0 cm  194.5 g
2)
3)
4)
5)
29.
30.
31.
30.
8
3
5
7
cm
cm
cm
cm
260.
322.
315.
328.
.0
.0
,0
.3
g
g
g
g

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SITE NAME: Ouachita River just below Arkansas/Louisiana state
           line

COUNTY/PARRISH, STATE: Union Parrish, Louisiana

LATITUDE/LONGITUDE: 33 00    92 04

SITE DESCRIPTION: Near mile marker 219

COLLECTION METHOD: Electrofishing

COLLECTORS: K. Cormier, M. Hutchins, C. Bates
            Louisiana Department of Environmental Quality

SAMPLING DATE: September 6, 1989

FISH COLLECTED:
   Sample #: DF025210
   Species: Channel Catfish (Ictalurus punctatus)
   Length and Weight:
   1) 43.5 cm  635 g
   2) 37.0 cm  454 g
   3) 39.9 cm  499 g

   Sample #: DF025211
   Species: Carp (Cyprinus carpio)
   Length and Weight:
   1) 51.0 cm  1769 g
   2) 54.5 cm  2313 g
   3) 57.5 cm  2540 g

   Sample #: DF025212
   Species: Largemouth Bass (Micropteris salmoides)
   Length and Weight:
   1) 37.5 cm  726 g
   2) 32.4 cm  499 g
   3) 30.5 cm  545 g

-------
SITE NAME: Bayou DeLoutre below the Arkansas/Louisiana State Line

COUNTY/PAKRISH, STATE: Union Parrish, Louisiana

LATITUDE/LONGITUDE: 32 42 45   92 10 19

SITE DESCRIPTION: Bayou DeLoutre north of Highway 2 at Phillip's
                  Ferry

COLLECTION METHOD: Electrofishing

COLLECTORS: K. Cormier, M. Hutchins, G.C. Bates, Louisiana
            Department of Environmental Quality

SAMPLING DATE: August 25, 1989

FISH COLLECTED:
   Sample #: DF025207
   Species: Largemouth Bass (Micropteris salmoides)
   Length and Weight:
   1)  46.9 cm  1701 g
   2)  38.1 cm  839 g
   3)  41.3 cm  1066 g
   4)  33.7 cm  567 g

   Sample #: DF025208
   Species: Black Bullhead (Ictalurus melas)
   Length and Weight:
   1)  33.0 cm  453.6 g
   2)  32.5 cm  453.6 g
   3)  30.5 cm  272.2 g

   Sample #: DF025209
   Species: Carp (Cyprinus carpio)
   Length and Weight:
   1)  61.6 cm  3175 g
   2)  50.8 cm  2041 g
   3)  59.1 cm  2812 g

-------
SITE NAME: Houston Ship Channel at Morgans Point

COUNTY/PARRISH, STATE: Harris County, Texas

LATITUDE/LONGITUDE: 29 40 58    94 58 55

SITE DESCRIPTION: East bank of Houston ship channel, just south
                  of Mary's bait camp, heavy cement rip-rap,
                  approximately two feet deep.

COLLECTION METHOD: 150 foot trotline with 25 4/0 hook stagings.
                   Oysters collected from rocks by hand.

COLLECTORS: R. Seiler, L. Broach, Texas Water Commission

SAMPLING DATE: August 3, 1989

FISH COLLECTED:
   Sample #: DF025309
   Species: Sea Catfish (Arius felis)
   Length and Weight:
   1) 25.4 cm  185.23 g
   2) 25.4 cm  151.46 g
   3) 24.13 cm  134.11 g
   4) 22.9 cm  90.38 g
   5) 25.4 cm  117.33 g
   6) 25.4 cm  144.83 g
   7) 22.9 cm  116.42 g
   8) 26.7 cm  180.25 g

   Sample #: DF025310
   Species: Oysters (Crassostrea virginica)
   Number of individuals: 36

   Note: Lab used only those oysters with intact shells  (about 30) .

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SITE NAME: Houston Ship Channel above San Jacinto Monument

COUNTY/PARRISH, STATE: Harris County, Texas

LATITUDE/LONGITUDE:  29 45 13    95 05 39

SITE DESCRIPTION: Sandy beach with gentle slope, no shoreline
                  vegetation, north bank of channel near CM 142
                  across from Patrick Bayou

COLLECTION METHOD: Gill net

COLLECTORS: R. Seiler, T. Jackson, Texas Water Commission

SAMPLING DATE: July 28, 1989

FISH COLLECTED:
   Sample #: DF025308
   Species: Blue Catfish (Ictalurus furcatus)
   Length and Weight:
   1)  23.6 cm  80 g
   2)  28.3 cm  150 g
   3)  28.7 cm  170 g

   Sample #: DF025307
   Species: Blue Crab (Callinectes sapidus) *
   Length and Weight:
1)
2)
3)
4)
5)
6)
7)
8)
9)
11.8
14.7
10.8
12.6
13.8
9.6
14.3
10.7
12.6
cm
cm
cm
cm
cm
cm
cm
cm
cm
120 g
155 g
75 g
110 g
135 g
70 g
140 g
85 g
120 g
      * Carapace measured from tip of spine to tip of spine.

-------
SITE NAME: Lake Sam Rayburn Headwaters

COUNTY/PARRISH, STATE: Angelina County, Texas

LATITUDE/LONGITUDE: 31 26 03   94 34 05

SITE DESCRIPTION: Lake Sam Rayburn  headwaters 3 1/2 miles above
                  Marion's Ferry

COLLECTION METHOD: Electroshocking

COLLECTORS: D. Ottmers, S. Meyers, P. Rogues, Texas Water
            Commission

SAMPLING DATE: August 31, 1989

FISH COLLECTED:
   Sample #:DF025301
   Species: Freshwater Drum (Aplodinotus grunniens)
   Length and Weight:
   1) 36.5 cm  520 g
   2) 33.5 cm  430 g

   Sample #: DF025302
   Species: Crappie (Pomoxis sp.)
   Length and Weight:
   1) 24 cm  250 g
   2) 18 cm  90 g

   Sample #: DF025303
   Species: Bluegill Sunfish (Lepomis macrochirus)
   Length and Weight:
   1) 17 cm  110 g
   2) 17 cm  100 g
   3) 17 cm  110 g
   4) 17 cm  100 g
   5) 17 cm  125 g
   6) 14.5 cm  70 g
   7) 13 cm  40 g

-------
SITE NAME: Neches River downstream of Temple Eastex - Evadale

COUNTY/PARRISH, STATE: Jefferson County, Texas

LATITUDE/LONGITUDE: 30 06 57   94 04 31

SITE DESCRIPTION: Neches River approximately 1/2 mile below
                  Temple Eastex discharge (canal). Predominantly
                  left bank (east - same side as discharge).

COLLECTION METHOD: Electroshocking

COLLECTORS: D. Ottmers, S. Meyer, P. Rogues,
            Texas Water Commission; C. Young, Environmental
            Protection Agency

SAMPLING DATE: August 30, 1989

FISH COLLECTED:
   Sample #: DF025304
   Species: Sunfish (Lepomis macrochirus and L. megalotis)
   Length and Weight:
   1) 11.0 cm  25 g (L. macrochirus)
   2) 11.5 cm  30 g (L. macrochirus)
   3) 11.5 cm  35 g (L. macrochirus)
   4) 11.5 cm  35 g (L. macrochirus)
   5) 13.0 cm  50 g (L. macrochirus)
   6) 13.0 cm  50 g (L. macrochirus)
   7) 11.0 cm  35 g (L. megalotis)
   8) 12.0 cm  40 g (L. megalotis)
   9) 13.0 cm  50 g (L. megalotis)
   10)  13.5 cm  60 g (L. megalotis)

   Sample #: DF025305
   Species: Largemouth Bass (Microterus salmoides)
   Length and Weight:
   1) 34.0 cm  560 g
   2) 29.0 cm  365 g
   3) 27.0 cm  285 g
   4) 26.5 cm  250 g
   5) 20.5 cm  120 g

   Sample #: DF025306
   Species: Spotted Gar (Lepisosteus oculatus)
   Length and Weight:
   1) 47.0 cm  395 g
   2) 47.5 cm  410 g
   3) 48.0 cm  420 g
   4) 45.5 cm  320 g
   5) 55.0 cm  730 g

-------
           Appendix 2
Quality Assurance Review of Data

-------
              UNITED STATES ENVIRONMENTAL PROTECTION AGENCY
                        REGION 6 - HOUSTON BRANCH
                           6608 HORNWOOD DRIVE
                           HOUSTON, TX  77074
ite
ase No.
                        ORGANIC QA CHECKLIST

     National Bioaccumulation         Contract No.

                                      Contractor _

                                      Matrix 	
                                                68-03-3423
        Study Sites
Work Assignment; 2-24
          TRILAB
          TL1-14270. 14341. 14418
eviewed by  Harry A. Kreiah - ESAT
ate 	December 14. 1989	
                                            Fish Tissue
ample No.  DF0252-07

           DF0252-08
           DF0252-09
           DF0252-13
                     DF0252-14
                     DF0252-15
                     DF0252-16
                     DF0252-17
                          DF0253-01
                          DF0253-02
                          DF0253-03
                          DF0253-04
DF0253-05
DF0253-06
DF0253-07
DF0253-08
VERALL COMMENTS (To Be Completed by EPA Personnel)

                               VOA        BNA

                               N/A        N/A
Holding Times

Tuning/Performance          N/A

Calibrations                N/A

Blanks                      N/A

Surrogates                  N/A

Matrix Spike/Duplicate      N/A

Compound Identity           N/A

Case Assessment             N/A
                                          N/A
                                          N/A
                                          N/A
                                          N/A

                                          N/A
                                          N/A
                                          N/A
                                          PEST

                                          N/A

                                          N/A

                                          N/A

                                          N/A

                                          N/A

                                          N/A

                                          N/A

                                          N/A
DF0253-09
DF0253-10
             Dioxin/
             Furan
               A

             1U/10P

               A
             1U/10P
DMMENTS OR CLARIFICATIONS  (See Attached)

 - Acceptable - All items delivered; all criteria met.	

 - Provisional - Data usable; some non-essential review items missing
   or criteria were not met.	  	
 - Unacceptable - Data unusable; essential review items missing
   or criteria not met.	
  - Not App1icab1e

-------
                         COMMENTS/CLARIFICATIONS
                            REGION 6 QA REVIEW

:ase WA: 2-24 TL1 14270.  14341.  14418    Site Region 6 Study     Lab TRILAB

[•he following is a summary of sample qualifiers used by Region  6
Ln reporting this CLP data:

  No.          Acceptable         Provisional         Unacceptable

  VOA             N/A	    	     	

  BNA             N/A	    	     	

  PEST       	N/A	    	     	
)ioxin/Furan  	7	     	10	     	1	

IOMMENTS:  The case consisted of 18 composite  fish tissue  samples  for
:etrachlorodibenzodioxins/furans by a modification of SW-846 Method  8290.
'he extracts were screened on a DB-225  column  and confirmed  on  a DB-5
:olumn.  The compounds  2,3,7,8-TCDD and/or 2,3,7,8-TCDF were reported  above
.0 ppt in samples DF0253-07, 09, and 10 (Houston Ship Channel samples).
   Sample DF0253-04 was  accidentally destroyed by the laboratory  prior  to
malysis and is unacceptable.  Data for 10 samples are provisional due to
>roblems with internal  standard and surrogate  recoveries,  and sample
.nterferences.  Data for  7 samples are  acceptable.  Requested laboratory
•esubmissions are enclosed in the data  package.

   Holding Times - Acceptable.  The fish samples were collected during  the
leriod from 7/28/89 to  8/30/89 and received by the laboratory on 8/22/89
md 9/6/89.  The samples  were extracted on 9/27/89 and 9/28/89  and analyzed
letween 10/19/89 and 10/31/89.  No contractual holding time  limits were
ipecified.

   Instrument Performance - Provisional.  The laboratory  met QC criteria
'or isomer resolution and SIM sensitivity.  Intervals between performance
iheck mixtures exceeded 12 hours and the performance check mixtures  were
iot analyzed at the end of each analytical sequence.  However,  the data
rere not affected.
   Total TCDF results  were flagged "E" in samples DF0252-15 and 16  and
(F0253-07, 08, 09, and  10 due to possible interferences by polychlorinated
iphenylethers.  Those  results are estimated as a consequence.
   Results for 2,3,7,8-TCDD are estimated in  samples DF0252-17, DF0253-
6, 08, and 09 because  the internal standard recoveries were <  40% on  both
olumns.  Results for 2,3,7,8-TCDD on the DB-5 column are  estimated  in
amples DF0252-13 and 14  because the internal  standard recoveries  were
 40%

   Calibrations - Acceptable.  Initial and routine calibrations met QC
riteria for %RSD and %D.  The raw data for the initial calibrations were
mitted from the data package, but were resubmitted by the laboratory.

-------
                          ORGANIC CLP/QA REVIEW
                            CONTINUATION PAGE

ASE NO.   WA;  2-24 TL1-14270. 14341.  14418          SITE  Region 6 Study

DMMENTS:

   Blanks - Acceptable.   Method blank # 1 contained 2,3,7,8-TCDF at 0.06
pt.  Associated samples  DF0252-07, 08,  and 09,  and DF0253-01 and 02 had
ery low 2,3,7,8-TCDF concentrations consistent  with blank contamination.
esults for 2,3,7,8-TCDF  are estimated in the affected samples.
   No method  blank analysis was performed on the DB-5 column, but results
or 2,3,7,8-TCDF on the DB-5 column were flagged "B" because of blank
ontamination  on the DB-225 column.  Method blank # 2 was not contaminated
y TCDD or TCDF.

   Surrogates - Provisional.  TCDD results for  sample DF0252-17 are
stimated because the surrogate recoveries were  < 40% on both columns.
CDD results for samples  DF0252-14 and DF0253-06 and 08 are estimated on
he DB-225 column because the surrogate recoveries were < 40%.  Other
arrogate recoveries were within QC control limits.

   Matrix Spike/Matrix Spike Duplicate - Acceptable.  MS/MSD recoveries
f 2,3,7,8-TCDD/TCDF met  QC criteria, but the MSD recovery of 2,3,7,8-TCDD
as marginally high (132%).  The MS/MSD recoveries demonstrated excellent
recision.

   Compound Identity/Data Completeness - Acceptable.  The compounds
,3,7,8-TCDD/TCDF were reported above 10 ppt in  samples DF0253-07, 09,  and
0.  TCDD and/or TCDF met identification criteria and were reported in all
amples except DF0252-08.  Results reported for  the DB-5 and DB-225 columns
ere very consistent.  The data package was complete upon receipt of
aboratory resubmissions.

   Case Assessment - Sample DF0253-04 is unacceptable because it was
ccidentally destroyed by the laboratory.
   Results for the following samples are provisional due to problems with
nternal standard and surrogate recoveries, and  sample interferences:
   DF0252-13, 14, 15, 16, 17
   DF0253-06, 07, 08, 09, 10
esults for 7  samples are acceptable.

-------
                                                                  ll
                                                                  /
                                                                jlli
December 14,  1989

Harry Kreigh
US EPA Region 6 Laboratory
6608  Hornwood
Houston, TX 77074

Dear  Mr. Kreigh:

     This   letter   is   in   response  to  your  questions   regarding
TCDF/TCDD analysis  in  reference  to  the TL1  Project  No.:  14270;
14341  and  14418.  The  responses  follow   structure  of  your  ques-
tions:
                                                        both  columns
 A. General.
 1.  Copies  of  raw  data for  initial  calibrations  on
 are enclosed.
 2.  The  initial  calibrations  were  developed  to  accommodate  all
 types  ("A","AA"  and  "B")  of  samples. These  give uniform response
 factors  and  calculation  formulas  for  all  analytes and  internal
 standards.  Surrogate  standard recoveries for  "A"  and  "AA"  type
 samples  are  calculates  differently  than those  of "B"  samples,
 however.   For   samples  "A"   and   "AA"  surrogate   are   calculated
 against  relevant internal  standards  and RRFs reported  for Initial
 Calibration may be applied directly.
      Surrogate  standards  of  the  "B"  samples,  such  as  the  fish
 tissue  in  question,  are   calculated  against  recovery  standards,
 therefore  both  surrogate  vs  internal  and  internal  vs  recovery
 standards  RRFs have  to be used  for the  computation. Moreover,  in
 the  case of 37CL4-TCDD only  one  (M/Z 332) peak  area  is utilized
 in calculation.
      The . surrogate  standard   recovery  for  the TLI  Blank (column
 DB225;   file  name  T894682;   I-Cal.   date  08/18/89)  serve   as  an
 example calculation:

 RRFs from the I-Cal.:
 37CL-TCDD (against 13C12-2.3, 7,8-TCDD -  IS1)-        2.19
 13C12-2,3,7,8-TCDD (against 13C12-1,2,3,4-TCDD -RSI)  1.02
Peak Areas:
37CLTCDD
13C12-1,2,3,4-TCDD (M./Z 332,  RT 15.08)
                                        11846; 53
                                        10800.02
                       11846.53
    Surrogate Recovery =
Reported value 49%.
                                            x  100 = 49%
                       10800.02 x 2.19  x 1.02
Similar  calculations  for  rest of  the samples  should  give correct
data as well.                   -- ___
                              Triangle Laboratories, Inc.
                              801-10 Cap/tola Drive
                              Research Triangle Park, NC 27713
                              919-544-5729

-------
      3.  The  12 hours interval  of an analytical  sequence should  be
regarded  as  a  time  which  elapses  between  Continuing Calibration
and  the   last  sample  in  a  series.  In  this  respect  none  of  the
sample  exceeds  the  aforementioned 12  hour  interval,  therefore
there  was no need  for  additional  Continuing Calibration analysis
at the  end of  analytical sequences.
                                                     1.3.2),  labora-
                                                     show concentra-
                                                     the theoretical
B.  DB225 Data.
     1. As  presented in the  Data User Manual  (&  5
tory method blank  is considered  valid if does  not
tion of a  specific  isomer  exceeding  one  third of
method quant.itation limit (TMQL).
     2,3,7,8-TCDF  TMQL  calculated  for  the Blank  #1  in question
(User  Manual  &  5.1.3.3)  is  0.19  ppt,  which  exceeds   3x  of  the
reported  0.06  ppt,   therefore  Blank  and   the  associated  samples
were  considered valid.
C.  DB5 Data.

     1.  DBS column  in TCDF/TCDD analyses  is used  exclusively  to
confirm  concentration  of  2,3,7,8-TCDD.  Since this  isomer was  not
found  in  the blank  analyzed with the  initial DB225  column,  con-
firmation was not warrant.
     The  "B" flag  is  used as  a  warning  that an  analyte has  been
found  in  the associate blank,  particularly  when  2,3,7,8-TCDF  was
identified using specific for  this analyte DB225 column.
     The  above  explanations should  facilitate  interpretation  of
the analytical data,  I hope.
     If  you have any  further questions or  comments please  contact
me  at (919) 544 5729.
                                  *

Sincerely,
                   ,
Jacek Bielawski, Ph~.D.
QC Manager

cc: Kike Me  Carthy
   Research Triangle Institute
   P.O. Box 12194
   Research Triangle Park, N.C. 27709
                              Triangle Laboratories, Inc.
                              801-10 Capitola Drive
                              Research Triangle Park, NC 27713
                              919-544-5729

-------
                    Appendix 3
Whole Body Fish Tissue Data for Smallmouth Buffalo
              from Red River  at  Index

-------
                   TRIANGLE LABORATORIES, INC.
                     801-10 CAPITOLA DRIVE
                 RESEARCH TRIANGLE PARK, NC  27713

                      Phone: (919) 544-5729
                        Fax: (919) 544-5491
DATE :

CLIENT ID :

P.O. NUMBER :

TLI PROJECT No.
      * 06 NOVEMBER 1989

      * RESEARCH TRIANGLE INSTITUTE

      * 10-434U-4365

      * 14270,14341,14418
                         CASE NARRATIVE
                          MODEL  8290X
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¥
¥
¥
¥
¥
¥
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¥
¥
¥
¥
¥  HIGH-RESOLUTION GAS CHROMATOGRAPHY /
¥                           HIGH-RESOLUTION MASS SPECTROMETRY
¥
ANALYSIS OF SAMPLES FOR  THE PRESENCE OF

   POLYCHLORINATED DIBENZO-p-DIOXINS
                   AND
              DIBENZOFURANS
                   BY
¥
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-------
Eighteen fish samples  were  received from RESEARCH TRIANGLE  INSTI-
TUTE in good condition September  6,  1989  and  stored  in  a  refrig-
erator at 4°C.   The samples were extracted  and  analyzed  accord-
ing to  procedures described  in  the Triangle Labs  User Manual
provided with this data package.   Any  particular difficulties
encountered during the sample handling by Triangle Labs will  be
discussed in the QA/QC remark  section below.

Qua!ity Assurance/Qua!ity Control Samples

A  laboratory  method blank  —  identified  as  the  TLI  Blank —  is
prepared along with the batch  of samples.

      Remarks
The release of this  particular set of RESEARCH TRIANGLE  INSTITUTE
analytical  data by  Triangle  Labs  was authorized by the  Quality
Assurance  Officer who  has reviewed  each sample  data package
individually following  a series  of inspections/reviews  conducted
at two other levels  of  the  data  production  line.    When applica-
ble, general  deviations  from  acceptable  QA/QC  requirements are
identified  below.     Comments  on the effect of  these deviations
upon the validity  and reliability  of  the  results can be obtained
from the User Manual  (Data  Quality Objectives; Section 5).
Specific QA/QC Problems Associated with this  Particular  Project
are:

Sample Preparation Laboratory: None

Mass Spectrometry: None

Data Review:

Matrix  spike  recoveries  and duplication  were  within quality
control  limits with  the single exception of 2,3,7,8-TCDD recovery
from sample DF0253-MS  (TLItt  22-15-12).   132% recovery is only
marginally above  the QC  limit (which is  130*),  and  the matrix
spike duplicate  presented similar recoveries within  the QC
criteria;  no  action  is  warranted.   Matrix spikes, matrix  spike
recoveries,  and  sample criteria for  the matrix spikes are
discussed  in  sections  5.1.3.6 and 5.2.2 of the Data User's
Manual.

Should RESEARCH TRIANGLE INSTITUTE have any questions or comments
to formulate  regarding this  data  package please  feel free to
contact us.

-------
For Triangle Laboratories,
Anal
QA Project Officer
Laboratory Manager
                    ^
Michael D. Chu  £«\Jacek Bie 11 awsfCi, Ph.D.     Diane  H.  Willifo

-------
               Appendix  3
Raw Data: TCDDs and TCDFs in Fish Tissue

-------
i 1 .   1 U.  O
 U i  • i o   i ivi
          1 I\ i rt IN Li L, C,  Una
                            TRIANGLE LABORATORIES, INC.
                          CONFIRMATION ANALYSIS (DB-5)
   FILE NAME	: T894965
   CONCAL	: T894958
   ANALYST	: PMC
   SAMPLE SIZE..
   ICAL DATE...-
   SPIKE FILE...
  20.20 g
08/21/89
SPCONF2K
CLIENT ID....: RTI
SAMPLE ID....: DF0252-16
ANALYSIS DATE: 10/31/89
SAMPLE MATRIX: FISH
SAMPLE ORIGIN: n/a
                                                                   Page 1 of 1
                                                                      11/13/89

                                                      TLI NUMBER....; 21-60-1

                                                      PROJECT NUMBER: 14341
                                                      DATE RECEIVED.: 09/06/89
                                                      DATE COLLECTED:   /  /
                                                      SHIPMENT NO...: n/o
NAME	         CONC(ppt) NUMBER      DL    EMPC
                            RATIO
                                                  RT
                                                                          FLAGS
2378-TCDD
2378-TCDF
TOTAL TCDD
TOTAL TCDF
6.6
4.0
6.6
4.0

1
1

6.9
6.6
0.77
0.77
0.77
0.77
20:42
20:08


-
	
_£_
   SURROGATE STD. RECOVERY SUMMARY (TYPE B)

   NAME             CONC (ppt)         X REC.

   37CL-TCDD             52.4            52.9
                                 RATIO
                                    RT

                                  20:42
                                                                      FLAGS
   INTERNAL STANDARDS RECOVERY SUMMARY
NAME
13C12-2378-TCDF
13C12-2378-TCDD
CONC (ppt)
70.8
43.6
X REC.
71.5
44.0
RATIO
0.78
0.78
RT
20:07
20:41
FLAGS
	
    Mississippi River - Zachary

    6 channel catfish (fillet)
                                                                CONF_RPT  rev:3.02

-------
                         TRIANGLE  LABORATORIES,  INC,
                       CONFIRMATION ANALYSIS  (DB-225)
                                             Page  1  of  1
                                                11/13/89
FILE NAME,...:
CQNCAL 	 	 :
ANALYST 	 t J
SAMPLE SIZE..
ICAL DATE....
SPIKE FILE...
NAME
2378-TCDD
2378-TCDF
TOTAL TCDD
TOTAL TCDF
Itjtf4oyj tLicni iu. . • • : no.
T894696 SAMPLE ID...,: DF0252-16
MV ANALYSIS DATE: 10/20/89
20.20 9 SAMPLE MATRIX: FISH
08/18/89 SAMPLE ORIGIN: n/a
SPCONF2K
CONC(ppt) NUMBER DL EMPC
6.2
4.6
6.2 1
5.3 3 8.8

PROJECT NUMBER: 14341
DATE RECEIVED.: 09/06/89
DATE COLLECTED: / /
SHIPMENT NO...: n/a
RATIO RT FLAGS
0.75 14:52
0.77 16:14 	
0.75 	
0.73 £
SURROGATE 8TD. RECOVERY SUMMARY (TYPE 8)

NAME	       CONC (ppt)         X REC.      RATIO
                                         RT

                                       14:52
                                                                      FLAGS
37CL-TCDD
61.4
                                      62.0
INTERNAL STANDARDS RECOVERY SUMMARY
NAME
13C12-2378-TCDF
13C12-2378-TCDD
CONC (ppt)
71.0
47.1
X REC.
71.7
47.5
RATIO
0.76
0.75
RT
16:13
14:51
FLAGS
	
       Mississippi River - Zachary

       6 channel catfish (fillet)
                                                             CONF_RPT rev:3.02

-------
11.  15.  89    01:18  PM    *TRIANGLE   LABS
                            TRIANGLE LABORATORIES,  INC.
                          CONFIRMATION ANALYSIS (DB-5)
FILE NAME	
CONCAL	
ANALYST	
SAMPLE SIZE..
ICAL DATE,...
SPIKE FILE...

NAME
                              CLIENT ID	:  RTI
                              SAMPLE ID....:  DF0252-17
                              ANALYSIS DATE:  10/31/89
                              SAMPLE MATRIX:  FISH
                              SAMPLE ORIGIN:  n/a
 TB94966
 T894958
 PMC
   20.28  g
 08/21/89
 SPCONF2K
:=r=r=sssrs=r:====-===================:
    CONC(ppt)  NUMBER      DL     CMPC
                                             Page 1 of 1
                                                11/13/89

                                TLI NUMBER....: 21-60-2

                                PROJECT NUMBER: 14341
                                DATE RECEIVED.: 09/06/89
                                DATE COLLECTED:   /  /
                                SHIPMENT NO...: n/a
                   RT
                                                          RATIO
                                                 FLAGS
   2378-TCDD
   2378-TCDF

   TOTAL TCDD

   TOTAL TCDF
1.5
8.4

1.5

8.4
9.2
                                                        0.89
                                                        0.78

                                                        0.89

                                                        0.78
                 20:43
                 20:08
   SURROGATE STD. RECOVERY SUMMARY (TYPE B)

   NAME
:— — -
-TC
CONC (ppt)
DO 30.4
X REC. RATIO
/ao'TS)
RT FLAGS
20:43 V
   INTERNAL STANDARDS RECOVERY SUMMARY
NAME
13C12-2378-TCDF
13C12-2378-TCDD
CONC (ppt)
44.5
26.8
* REC.
45,2
£2l,P
RATIO
0.78
0.78
RT
20:07
20:42
FLAGS
"\T
    Mississippi River - Zachary

    4 white crappie (fillet)
                                                                CONF_RPT rev:3.02

-------
L.I.  i b.
U l :  1 o
o
                            TRIANGLE LABORATORIES,  INC.
                          CONFIRMATION ANALYSIS (DB-225)
                                                    Pago  1  of  1
                                                       11/13/89
FT 1 F NAME . •
CONCAL 	 :
ANALYST 	 :
SAMPLE SIZE..:
ICAL DATE...,:
SPIKE FILE...:
NAME
2378-TCDD
2378-TCDF
TOTAL TCDD
TOTAL TCDF
SURROGATE STD.
NAME
37CL-TCDD
T894694 CLIENT ID....: RTI
T894678 SAMPLE ID 	 : DF0262-17
MV ANALYSIS DATE: 10/20/89
20.28 g SAMPLE MATRIX: FISH
08/18/89 SAMPLE ORIGIN: n/a
SPCONF2K
CONC (ppt) NUMBER DL EMPC
1.4
9.8
1.4 1
11.2 4 11,4
RECOVERY SUMMARY (TYPE B)
CONC (ppt) X REC. , RATIO
33.6 '34.1
TLI NUMBER 	 :

PROJECT NUMBER:
DATE RECEIVED.:
DATE COLLECTED:
SHIPMENT NO...:
•ff2ES£ttt""fl!»*' — ~~
RATIO RT
0.74 14:52
0.75 16:15
0.74
0.78
:rS±BSKSSS::z'' = SZ
RT
14:52
21-60-2

14341
09/06/89
/ /
n/a
FLAGS
— — — aass
FLAGS
v
   INTERNAL STANDARDS RECOVERY SUMMARY
NAME
13C12-2378-TCDF
13C12-2378-TCDD
CONC (ppt)
44.2
27.1
X REC.
44. 8. v
;'27.5
^
RATIO
0.75
0.75
RT
16:14
14:51
FLAGS

V
          Mississippi River - Zachary

          4 white  crappie (fillet)
                                                                CONFJRPT rev:3.02

-------
   15.  89     01:18   PM    *TRI ANGLE  LABS
                         TRIANGLE LABORATORIES, INC,
                       CONFIRMATION ANALYSIS (DB-5)
FILE NAME	:  T694960
CONCAL	:  T894958
ANALYST	:  PMC
SAMPLE SIZE..:    20.11 g
ICAL DATE.,..:  08/21/89
SPIKE FILE..,:  SPCONF2K
CLIENT ID	: RTI
SAMPLE ID....: DF0252-13
ANALYSIS DATE: 10/31/89
SAMPLE MATRIX: FISH
SAMPLE ORIGIN: n/a
                                             Page 1 of 1
                                                11/13/89

                                TLI NUMBER....: 22-15-7

                                PROJECT NUMBER: 14418
                                DATE RECEIVED.: 09/06/89
                                DATE COLLECTED:   /  /
                                SHIPMENT NO...: n/a
NAME
2378-TCDD
2378-TCDF
TOTAL TCDD
TOTAL TCDF
CONC (ppt) NUMBER
7.4
1.7
7.4 1
1.7 1
DL EMPC RATIO
0.82
0.76
0.82
0.76
5SC====
RT
20:41
20:07


FLAGS
JL
_.
	
SURROGATE STD. RECOVERY SUMMARY (TYPEJ)		

    =S=SS"SSSSSS=	i~REc"RATIO
37CL-TCDD
39.6
           39.8
  RT     FLAGS

20:41     _y_
INTERNAL STANDARDS RECOVERY SUMMARY
NAME
13C12-2378-TCDF
13C12-2378-TCDD
CONC (ppt)
69.5
38.8
X REC.
69.9
£39rtp
RATIO
0.77
0.77
RT
20:06
20:40
FLAGS

V
Anacoco Creek

5 channel catfish  (fillet)
                                                             CONF_RPT rev:3.02

-------
11.   1 •-> .
                             TRIANGLE LABORATORIES, INC,               Page 1 of 1
                           CONFIRMATION ANALYSIS (DB-225)       ,          11/13/89

    FILE NAME....: TS94692     CLIENT ID....: RTI         TLI NUMBER....: 22-15-7
    CONCAL	: T894678     SAMPLE ID....: DF0252-13
    ANALYST	: MV          ANALYSIS DATE: 10/20/89    PROJECT NUMBER: 14418
    SAMPLE SIZE..:   20.11 g   SAMPLE MATRIX: FISH        DATE RECEIVED.: 09/06/89
    ICAL DATE....: 08/18/89    SAMPLE ORIGIN: n/a         DATE COLLECTED:   /  /
    SPIKE FILE...: SPCONF2K                               SHIPMENT NO...: n/a

    NAME              CONC(ppt) NUMBER      DL    EMPC     RATIO     RT    FLAGS*""

    2378-TCDD             7.4                               0.72   14:52    	
    2378-TCDF             1.8                               0.78   16:14    J^

    TOTAL TCDD            7.4      1                        0.72            	

    TOTAL TCDF            1.8      1              1.9       0.78


    SURROGATE STD.  RECOVERY SUMMARY (TYPE B)

    NAME             CONC (ppt)         X REC.      RATIO          RT     FLAGS

    37CL-TCDD             49.7            49.9                   14:51
    INTERNAL STANDARDS RECOVERY SUMMARY

    NAME             CONC (ppt)         X REC.       RATIO          RT     FLAGS

    13C12-2378-TCDF       74.4            74.8        0.76        16:13     	
    13C12-2378-TCDD       43.7            44.0        0.75        14:51     	

                                                                 CONF_RPT rev:3.02
          Anacoco Creek

          5 channel catfish (fillet)

-------
i.  i b.
I) 1 :  i o
                                    'Is 1 I\ 1 fl M NJ
                           TRIANGLE LABORATORIES,  INC,
                         CONFIRMATION ANALYSIS (DB-5)
  FILE NAME....:
  CONCAL	:
  ANALYST	:
  SAMPLE SIZE..:
  ICAL DATE	:
  SPIKE FILE,..:
T894961
T894958
PMC
  20.08 9
08/21/Bfl
SPCONF2K
CLIENT ID,.,.: RTI
SAMPLE ID....: DF0252-14
ANALYSIS DATE: 10/31/89
SAMPLE MATRIX: FISH
SAMPLE ORIQIN: n/8
                                          :==ssssss=ss
  NAME
   CONC(ppt) NUMBER      DL    EMPC
             Page 1 of 1
                11/13/89

TLI NUMBER....: 22-15-8

PROJECT NUMBER: 14418
DATE RECEIVED.: 09/06/89
DATE COLLECTED:   /  /
SHIPMENT NO...: n/a

 RATIO     RT     FLAGS
  2378-TCDD
  2378-TCDF

  TOTAL TCDD

  TOTAL TCDF
       1,5
       2.2

       1.5

       2.2
                             0.74
                             0.80

                             0.74

                             0.80
          20:42
          20:07
  SURROGATE STD. RECOVERY SUMMARY^(TYPE_B)	Kaass.SSBSSSr~BS«=«===s
  ============================-=-====-----      ----"         DT      PIAftR
  NAME

  37CL-TCDD
        39.1
            39.3
                                                 RT

                                               20:41
                                            FLAGS

                                              V
   INTERNAL  STANDARDS  RECOVERY SUMMARY
   ^J_L,._.» — •••^^•^••^~~^~^^^ — — ••^•••••r«-'^~ — "••"•~— *~*"

   NAMECONC  (ppt)         X REC.
                                                 RT
                                            FLAGS
                                                    0.79
   13C12-2378-TCDF       52.2                ,
   13C12-2378-TCDD       39.4          /J9.&       0.79
                                               20:06
                                               20:40
                                                                CONF^RPT rev:3.02
   Anacoco Creek

   5  longear sunfish (fillet)

-------
                        TRIANGLE LABORATORIES, INC.
                       CONFIRMATION ANALYSIS (DB-225)
               ,^jefi.      riTPNT TD     • RTI         TLI NUMBER,..,: 22-15-8
             •  T&94691      CLlcNI AU...». nn
         : : : : ;  T^BTB      SAMPLE ID  , . . ; DF0252- 4        cj NUMBER= ^
ANALYST ...... :  MV          JNALYS IS DATE. W20/B8    DATE RECEIVED.: 09/06/89
SAMPLE SIZE,,:    20.08  9   SAMPLE MATRIX. FISH             COLLECTED:   /  /
ICAL DATE..,.:  08/18/89    SAMPLE ORIGIN, n/a         SHIPHENT NO...: n/a
SPIKE FILE...:  SPCONF2K         .............. =S-S======S2=====«======"«==
     s2==SSE=====
 2378-TCDD           EMPC                       l         Qi76   16:16
 2378-TCDF             2'4

 TOTALTCDD          *1                    ^'
 INTERNAL STANDARDS RECOVERY_SUMMARY _____ ==.======.===s==s====r==r===="="=
 s====:=================:::::''===::=c   I'ocr"     RATIO          RT     FLAGS
 NAME             CONC (ppt)     _   X REC^      KAUO
                                 _
                                       C-ST       074        16:13     _
  13C12-2378-TCDF       53,5            M.j        •           U:51     __
  1 3C 1 2-237 8-TCDD       ^0 • 5          __40 • '_ ____________________________________
  ----------------------------                                 CONF.RPT rev: 3. 02


        Anacoco Creek

        5 longear  sunfish (fillet)

-------
i 1 .   i -->.
                            TRIANGLE LABORATORIES, INC.
                          CONFIRMATION ANALYSIS (DB-5)
   FILE NAME....: T894962
   CONCAL	: T894958
   ANALYST	: PMC
   SAMPLE SIZE..:   20.16 g
   ICAL DATE	: 08/21/89
   SPIKE FILE...: SPCONF2K
     CLIENT ID....: RTI
     SAMPLE ID....: DF0252-15
     ANALYSIS DATE: 10/31/89
     SAMPLE MATRIX: FISH
     SAMPLE ORIGIN: n/a
                             Page 1 of 1
                                11/13/89

                TLI NUMBER	: 22-15-9

                PROJECT NUMBER: 14416
                DATE RECEIVED.: 09/06/89
                DATE COLLECTED:   /  /
                SHIPMENT NO,.,: n/a
NAME
2378-TCDD
2378-TCDF
TOTAL TCDD
TOTAL TCDF
CONC (ppt)
1.6
2.9
1,8
2,9
NUMBER

1
1
DL EMPC


3.0
RATIO
0.66
0.81
0.66
0.81
ZSSS5SSS
RT
20:41
20:06


FLAGS
JL
___
J_
   SURROGATE STD. RECOVERY SUMMARY (TYPE B)

   NAME             CONC (ppt)         X REC.       RATIO          RT     FLAGS
   37CL-TCDD
52.1
52.5
20:40
   INTERNAL STANDARDS RECOVERY SUMMARY
NAME
13C12-2378-TCDF
13C12-2378-TCDD
CONC (ppt)
82.1
49.5
X REC.
82.7
49.9
RATIO ^
0.77
0.78
RT
20:06
20:40
FLAGS
,^^_
    Anacoco Creek

    5 mullet (fillet)
                                                                CONF_RPT rev:3.02

-------
ii.
        o y
u 1:10
                                                                            f 1 U
                         TRIANGLE LABORATORIES, INC.
                       CONFIRMATION ANALYSIS (DB-225)
                                                                           1  of 1
                                                                         11/13/89
FILE NAME....: T894690
CONCAL	: TBS4678
ANALYST	 MV
SAMPLE SIZE..:   20.16 g
ICAL DATE	: OB/18/89
SPIKE FILE...: SPCONF2K
                              CLIENT ID	  RTI
                              SAMPLE ID	:  DFD252-15
                              ANALYSIS DATE:  10/20/89
                              SAMPLE MATRIX:  FISH
                              SAMPLE ORIGIN:  n/a
                                      TLI NUMBER....: 22-15-9

                                      PROJECT NUMBER: 14418
                                      DATE RECEIVED.: 09/06/89
                                      DATE COLLECTED:   /  /
                                      SHIPMENT NO,..: n/a
NAME
2378-TCDD
2378-TCDF
TOTAL TCDD
TOTAL TCDF
CONC (ppt)
1.6
3.3
1.6
3.3
NUMBER DL

1
1
EMPC RATIO
0,66
0.73
0.66
3,4 0.73
"RT
14:52
16:15


FLAGS
HI
__
e
SURROGATE STD.  RECOVERY SUMMARY (TYPE B)

NAME             CONC (ppt)         X REC,       RATIO          RT     FLAGS
37CL-TCDD
                         59.2
                   -  59.7
14:52
INTERNAL STANDARDS RECOVERY SUMMARY
NAME
13C12-2378-TCDF
13C12-2378-TCDD
CONC (ppt)
77.8
52.2
X REC.
78.4
52.6
RATIO
0.72
0.75
RT
16:14
14:51
FLAGS
	
     Anacoco Creek

     5 mullet (fillet)
                                                             CONF_RPT rev:3.02

-------
                         TRIANGLE  LABORATORIES,  INC.                Pago  1 of  1
                        CONFIRMATION ANALYSIS  (DB-225)                  11/13/89

 FILE  NAME...,: T8946B4     CLIENT  10,...: RTI         Til NUMBER....:  22-15-1
 CONCAL	: T894678     SAMPLE  ID..,,: DF0252-07
 ANALYST	: JAJ         ANALYSIS DATE: 10/19/89    PROJECT NUMBER:  14418
 SAMPLE SIZE..:   20.20  g   SAMPLE  MATRIX: FISH        DATE RECEIVED.:  09/06/89
 ICAL  DATE....: 08/18/89    SAMPLE  ORIGIN: n/a         DATE COLLECTED:   /  /
 SPIKE FILE.,,: SPCONF2K                               SHIPMENT NO...:  n/a

 NAME              CONC(ppt) NUMBER      DL    EMPC     RATIO     RTFLAGS*"

 2378-TCDD            ND               0.04                              _1_
 2378-TCDF             0.19                              0.72   16:15     B

 TOTAL TCDD           ND               0.04                              	

 TOTAL TCDF            0.19     1                        0.72            	


 SURROGATE STD. RECOVERY SUMMARY (TYPE B)

 NAME             CONC (ppt)         X REC.      RATIO          RT     FLAGS

 37CL-TCDD             46.4            46.9                   14:52     	


INTERNAL STANDARDS RECOVERY SUMMARY

NAME             CONC (ppt)         % REC.      RATIO          RT     FLAGS

13C12-2378-TCDF       84.3            85.1       0.76        16:14     	
 13C12-2378-TCDD       41.2            41.6       0.75        14:51     	

                                                             CONF_RPT rev:3.02


      Bayou DeLoutre

      4 largemouth bass (fillet)

-------
11.   ID.  O
U 1 •  i O  r ivi
                                           J. rt H U LF C.  n n i^ •-»,
                              TRIANGLE LABORATORIES,  INC.
                            CONFIRMATION ANALYSIS (DB-225)
     FILE NAME....:  T894685
     CONCAL	:  T894678
     ANALYST	:  JAJ
     SAMPLE SIZE.,:    20.19  0
     ICAL DATE.,..:  08/18/89
     SPIKE FILE...:  SPCONF2K
             CLIENT ID....: RTI
             SAMPLE ID,,,,: DF0252-08
             ANALYSIS DATE: 10/19/89
             SAMPLE MATRIX: FISH
             SAMPLE ORIGIN: n/a
                              Page  1  of 1
                                 11/13/89

                TLI NUMBER....:  22-15-2

                PROJECT NUMBER:  14418
                DATE RECEIVED.:  09/06/89
                DATE COLLECTED:   /  /
                SHIPMENT NO...:  n/fl
    NAME              CONC(ppt) NUMBER       DL     EMPC    "  RATIO	RT     FLAQs"
    2378-TCDD
    23T8-TCDF

    TOTAL TCDD

    TOTAL TCDF
       ND
      EMPC

       ND

      EMPC
0.02
0.02
        0,07
        0,07
    SURROGATE STD. RECOVERY SUMMARY  (TYPE B)

    NAME             CONC (ppt)         % REC.      RATIO          RT      FLAGS*
    37CL-TCDD
        52.0
52.5
14:52
    INTERNAL STANDARDS RECOVERY SUMMARY
NAME
13C12-2378-TCDF
13C12-2378-TCDD
CONC (ppt)
83,1
46.7
% REC.
83.9
47.2
RATIO
0.75
0.75
RT
16:14
14:51
FLAGS
	
          Bayou DeLoutre

          3 black bullhead  (fillet)
                                                                 CONF_RPT rev:3.02

-------
li.   i u.  uo     ui-ii-1   A ivi    •>< i i\ ji n 11
                             TRIANGLE LABORATORIES, INC,               Page 1 Of 1
                           CONFIRMATION ANALYSIS (08-225)                 11/13/89

    FILE NAME....: T894686     CLIENT ID....: RTI         TLI NUMBER....: 22-15-3
    CONCAL	: T89467B     SAMPLE ID....: DF0252-09
    ANALYST	: HV          ANALYSIS DATE: 10/20/89    PROJECT NUMBER: 14418
    SAMPLE SIZE..:   20.26 g   SAMPLE MATRIX: FISH        DATE RECEIVED.: 09/06/89
    ICAL DATE....: 08/18/89    SAMPLE ORIGIN: n/a         DATE COLLECTED:   /  /
    SPIKE FILE...: SPCONF2K                               SHIPMENT NO...: n/a

    NAME              CONC(ppt) NUMBER      DL    EMPC     RATIO     RT ""FLAGS"
2378-TCDD
2378-TCOF
TOTAL TCDD
TOTAL TCDF
NO
0.33
ND
0.33 1
0.04

0.04


0.81 16:15

0.81

X
m^imm

    SURROGATE STD.  RECOVERY SUMMARY (TYPE B)

    NAME              CONC  (ppt)          X REC.       RATIO          RT     FLAGS
    37CL-TCDD             45.4            46.0                   14:53     	


    INTERNAL  STANDARDS  RECOVERY SUMMARY

    NAME              CONC  (ppt)         X  REC.       RATIO          RT     FLAGS

    13C12-2378-TCDF        64.6            65.4       0.75        16:14     	
    13C12-2378-TCDD        40.4            41.0       0.77        14:51     	

                                                                 CONF_RPT rev:3.02

          Bayou DeLoutre

          3 carp (fillet)

-------
1.  ID.
U 1 :  i  o
i i i\
         n
                          TRIANGLE LABORATORIES, INC.
                        CONFIRMATION ANALYSIS (DB-5)
 FILE NAME.,,.: T894967
 CONCAL	: T894958
 ANALYST	: PMC
 SAMPLE SIZE..:   SO.25 g
 ICAL DATE....: 08/21/89
 SPIKE FILE...: SPCONF2K

 NAME              CONC(ppt) NUMBER      DL    EMPC
            CLIENT  ID....: RTI
            SAMPLE  ID....: DF0253-07
            ANALYSIS  DATE: 10/31/89
            SAMPLE  MATRIX: FISH
            SAMPLE  ORIGIN: n/a
                                 Page 1 of 1
                                    11/13/89

                    TLI NUMBER....:  21-3-1

                    PROJECT NUMBER:  14270
                    DATE RECEIVED.:  09/06/89
                    DATE COLLECTED:    /  /
                    SHIPMENT NO...:  n/a
                  :S========s===r=s=========
                     RATIO     RT    FLAGS
2378-TCDO
2378-TCDF
TOTAL TCDD
TOTAL TCDF
54.8
85.8
66.2
140

7
13

66.5
143
0.84
0.76
0.79
0.76
20:42
20:08


	
—
J_
 SURROGATE STD. RECOVERY SUMMARY (TYPE B)

 NAME             CONC (ppt)         X REC.

 37CL-TCDD             62.3            63.0
                                RATIO
                             RT
FLAGS
                                             20:42
 INTERNAL STANDARDS RECOVERY SUMMARY
NAME
13C12-2378-TCDF
13C12-2378-TCDD
CONC (ppt)
84.6
54.6
X REC.
85.7
55.3
RATIO
0.78
0.76
RT
20:07
20:42
FLAGS
- —
  Houston Ship Channel

  9 blue crabs
                                                              CONF.RPT rev:3.02

-------
1.  15.
U i :  i  o  rivi
                                                                               i u
                          TRIANGLE LABORATORIES,  INC.
                        CONFIRMATION ANALYSIS (DB-225)
                                                   Page 1 of 1
                                                      11/13/89
 FILE NAME	:  T894699
 CONCAL	:  T894696
 ANALYST	:  MV
 SAMPLE SIZE..
 ICAL  DATE....
 SPIKE FILE...
           CLIENT ID	: RTI         TLI NUMBER....: 21-3-1
           SAMPLE ID....: DF02S3-07
           ANALYSIS DATE: 10/20/89    PROJECT NUMBER: 14270
                                      DATE RECEIVED.: 09/06/89
                                      DATE COLLECTED:   /  /
  20.25 g   SAMPLE MATRIX: FISH
08/18/89    SAMPLE ORIGIN: n/a
SPCONF2K
                                      SHIPMENT NO...: n/a
 NAME              CONC(ppt)  NUMBER      DL     EMPC     RATIO     RT     FLAGS
2378-TCDD
2378-TCDF
TOTAL TCDD
TOTAL TCDF
50,6
96.1
56.7
169

5
22

59.1
171
0.76
0.76
0.75
0.75
14:52
16:15


	
^.^
f
 SURROGATE  STD. RECOVERY SUMMARY  (TYPE B)

 NAME             CONC  (ppt)         X REC.      RATIO          RT      FLAGS
 37CL-TCDD
      73.4
                       74.4
14:52
 INTERNAL  STANDARDS RECOVERY SUMMARY
NAME
13C12-2378-TCDF
13C12-2378-TCDD
CONC (ppt)
85.3
60.3
* REC.
86.4
61.1
RATIO
0.74
0.74
RT
16:13
14:51
FLAGS
	
      Houston Ship Channel

      9 blue crab
                                                             CONF_RPT rev:3.02

-------
1 .   i b .
               u i :  i o
                                        i n 11 \j xj i-   u • * ~ »
                         TRIANGLE LABORATORIES, INC.
                       CONFIRMATION ANALYSIS (DB-5)
FILE NAME	: T894968
CONCAL	: T894958
ANALYST	: PMC
SAMPLE SIZE..:   20.21 g
ICAL DATE,...: 08/21/89
SPIKE FILE,..: SPCONF2K
                             CLIENT ID....:  RTI
                             SAMPLE ID....:  DF0253-08
                             ANALYSIS  DATE:  10/31/89
                             SAMPLE MATRIX:  FISH
                             SAMPLE ORIGIN:  n/a
             Page 1 of 1
                11/13/89

TL1 NUMBER....: 21-3-2

PROJECT NUMBER: 14270
DATE RECEIVED.: 09/06/89
DATE COLLECTED:   /  /
SHIPMENT NO...: n/a
NAME
2378-TCDD
2378-TCDF
TOTAL TCDD
TOTAL TCDF
CONC (ppt) NUMBER
3.2
0.80
3.2 1
0.80 1
s======== ===============
DL EMPC RATIO
0.70
0,67
0.70
1.3 0,67
RT FLAGS
20:41 	
20:07 	
__
_e
SURROGATE STD. RECOVERY SUMMARY (TYPE B)

NAME             CONC (ppt)         X REC.

37CL-TCDD             36.5          £36.?


INTERNAL STANDARDS RECOVERY SUMMARY
                                                RATIO
 Houston Ship Channel

 3 blue catfish (fillet)
                                                                 RT

                                                               20:41
                FLAGS
NAME
13C12-2378-TCDF
13C12-2378-TCDD
CONC (ppt)
44.8
31. A
% REC.
45.3
RATIO
0.78
0.76
RT
20:06
20:40
FLAGS
3E
                                                             CONFJ*PT rev:3,02

-------
  . i o.  o a
                u i .  i o  r ivi
                                                     UrtDO
                                                                            r i
                         TRIANGLE LABORATORIES, INC.
                       CONFIRMATION ANALYSIS (DB-225)
FILE NAME....:
CONCAL	',
ANALYST	:
SAMPLE SIZE..:
ICAL DATE....:
SPIKE FILE...:
T894700
T894696
MV
  20.21 g
08/18/89
SPCONF2K
CLIENT ID....: RTI
SAMPLE ID	: DF0253-08
ANALYSIS DATE: 10/20/89
SAMPLE MATRIX; FISH
SAMPLE ORIGIN: n/a
             Page 1 of 1
                11/13/89

TLI NUMBER....: 21-3-2

PROJECT NUMBER: 14270
DATE RECEIVED.: 09/06/89
DATE COLLECTED:   /  /
SHIPMENT NO...: n/a
NAME
2378-TCDD
2378-TCDF
TOTAL TCDD
TOTAL TCDF
CONC(ppt) NUMBER
2.9
0.78
2.9 1
0.78 1
DL EMPC RATIO RT
0.73 14:51
0.76 16:13
0.73
1.8 0.76
FLAGS
	
^ ^^_
_£_
SURROGATE STD.  RECOVERY SUMMARY (TYPE B)

NAME             CONC (DDt)         K REC.       RATIO
         RT

       14:51
                                                       FLAGS
37CL-TCDD
       41.4
           41.9
INTERNAL STANDARDS RECOVERY SUMMARY
NAME
13C12-2378-TCDF
13C12-2378-TCDD
CONC (ppt)
45.2
32.2
X REC.
45.7
RATIO
0.75
0.75
RT
16:13
14:51
FLAGS
V
                                                             CONF_RPT rev:3.02
      Houston Ship Channel

      3 blue catfish (fillet)

-------
1.  15.  89     01:18   PM    *TRIANGLE  LAbb
                                                r o
                          TRIANGLE  LABORATORIES,  INC.
                         CONFIRMATION  ANALYSIS  (DB-5)
                                        Page 1 of 1
                                           11/13/89
  FILE  NAME.,..: T894989
  CONCAL	: T894958
  ANALYST	: PMC
CLIENT ID.,..! RTI         TLI NUMBER	: 21-3-3
SAMPLE ID	: DF0253-09
ANALYSIS DATE: 10/31/89    PROJECT NUMBER: 14270
  SAMPLE  SIZE..:   20.05  g   SAMPLE MATRIX:  FISH         DATE RECEIVED.:  09/06/89
  ICAL  DATE....: 08/21/89   SAMPLE ORIGIN:  n/a          DATE COLLECTED:    /  /
  SPIKE FILE...: SPCONF2K                               SHIPMENT NO...:  n/a

  NAME              CONC(ppt) NUMBER      DL   EMPC     RATIO     RT     FLAGS

  2378-TCDD             14.8                               0.80   20:41     	
  2378-TCDF             1.7                               0,67   20:06     	

  TOTAL TCDD            14.8       1                        0.80             _..

  TOTAL TCDF            1.7       1              5.3       0.67


  SURROGATE STD. RECOVERY SUMMARY (TYPE B)

  NAME             CONC (ppt)         X REC.      RATIO          RT      FLAGS

  37CL-TCDD             39.9            40.0                  20:41      	


  INTERNAL STANDARDS RECOVERY SUMMARY

  NAME             CONC (ppt)         X REC.      RATIO          RT      FLAGS

  13C12-2378-TCDF       47.3            47.4      0.79        20:06      ._
  13C12-2378-TCDD       32.9          ^33.th      0.76        20:40       V
                                     ^^..—..— -

                                                              CONF.RPT  rev:3.02
  Houston Ship Channel

  8 sea  catfish (fillet)

-------
1. 1 5. 89  0 1 : 1 B
T K 1 H 1N
               TRIANGLE LABORATORIES, INC.
              CONFIRMATION ANALYSIS (DB-225)
                  Page 1 of 1
                   11/13/89
 FILE NAME  - T894702   CLIENT ID....: RTI     TLI NUMBER....: 21-3-3
 ssfc::£ sr;,  SH?iSF  SSMKIIWU
 s™»" •$&  ISI=;"'     SSWSHnn/. '
or inc. r*u*».«." — •
ssts=r======s=s=!
NAME
s-ss = ss= =========== ==""===:
CONC (ppt) NUMBER DL
2378-TCDD 14-j
2378-TCDF ^6
TOTAL TCDD 14-4 1
TOTAL TCDF 1»6 1
SURROGATE STD. RECOVERY SUMMARY (TYPE B)
NAME
CONC (ppt) « REC.
37CL-TCDD 44.7 44.9
INTERNAL STANDARDS RECOVERY SUMMARY
NAME
13C12-2378-TCDF
13C12-2378-TCDD
CONC (ppt) % REC.
48.0 48* JU
34.8 Q£iS-
EMPC RATIO RT
0.76 14:51
0.83 16:14
0.76
6.4 0.83
_._. 	 - = £- = = = --- = = 3Z = S = S!
RATIO RT
14:51
RATIO RT
0.74 16:13
> 0.75 14:50
FLAGS
J-
>se-s==
FLAGS

FLAGS
"y"
                                    CONF RPT rev:3.02
  Houston Ship Channel

  8 sea catfish (fillet)

-------
ii.   io.  o y
u i :  i o  nvi
*TKiANULt   LttBS
                 Jb
                            TRIANGLE LABORATORIES,  INC.
                          CONFIRMATION ANALYSIS (DB-5)
   FILE NAME	:  T894972
   CONCAL	:  T894971
   ANALYST	:  DM
   SAMPLE SIZE..:    20.25 9
   ICAL DATE....:  08/21/89
   SPIKE FILE...:  SPCONF2K
            CLIENT ID,,..: RTI
            SAMPLE ID....: DF0253-10
            ANALYSIS DATE: 10/31/89
            SAMPLE MATRIX: FISH
            SAMPLE ORIGIN: n/a
                                 Page 1  of 1
                                    11/13/89

                   TLI  NUMBER....:  21-3-4

                   PROJECT  NUMBER:  14270
                   DATE RECEIVED.:  09/06/89
                   DATE COLLECTED:    /   /
                   SHIPMENT NO...:  n/a
NAME
2378-TCDD
237B-TCDF
TOTAL TCDD
TOTAL TCDF
===r=cr=======
CONC(ppt)
6.1
14.9
13.2
44.7
NUMBER

6
15
DL EMPC

17,0
45.0
RATIO
0.87
0,79
0.79
0,78
RT
20:46
20:11


FLAGS
	
	
t:
   SURROGATE STD.  REC6VERY SUMMARY (TYPE B)

   NAME             CONC (ppt)          % REC.

   37CL-TCDD             74,9            75.8
                                RATIO
                            RT

                          20:46
         FLAGS
   INTERNAL STANDARDS RECOVERY SUMMARY

   NAME             CONC  (ppt)         X  REC.
                                RATIO
                            RT
         FLAGS
   13C12-2378-TCDF        88.7
   13C12-2378-TCDD        62.1
                      89.8
                      62.9
              0.77
              0.77
20:11
20:45
    Houston Ship Channel

    36  oysters
                                                                CONF_RPT  rev:3.02

-------
1.  1 b.
U 1 :  i  0
                                      unu *j
                          TRIANGLE LABORATORIES, INC,
                        CONFIRMATION ANALYSIS (DB-225)
 FILE NAME....: T894701
 CONCAL	: T894696
 ANALYST	: MV
 SAMPLE SIZE..:   20.25 g
 ICAL DATE....: 08/18/89
 SPIKE FILE...: SPCONF2K
            CLIENT  ID	:  RTI
            SAMPLE  ID,,..:  DF0253-10
            ANALYSIS DATE:  10/20/89
            SAMPLE  MATRIX:  FISH
            SAMPLE  ORIGIN:  n/a
                                     Page 1 of 1
                                        11/13/89

                        TLI NUMBER....: 21-3-4

                        PROJECT NUMBER: 14270
                        DATE RECEIVED.: 09/06/89
                        DATE COLLECTED:   /  /
                        SHIPMENT NO...: n/a
 NAME	"       CONC(ppt) NUMBER      DL    EMPC     RATIO     RT    FLAGS
  2378-TCDD
  2378-TCDF

  TOTAL TCDD

  TOTAL TCDF
       5.8
      14.9

      12.1

      46.1
. 6

20
15.4

50.3
0.74   14:52    	
0,78   16:14    	

0.73            	

0,74             £
  SURROGATE STD. RECOVERY SUMMARY  (TYPE B)

  NAMECONC  (ppt)         X REC.      RATIO          RT      FLAGS
  37CL-TCDD
       86.6
        87.7
                                                              14:51
  INTERNAL STANDARDS RECOVERY SUMMARY

  NAME	  CONC  (ppt)         * REC.      RATIO          RT      FLAGS
  13C12-2378-TCDF       89.2
  13C12-2378-TCDD       65.8
                       90.3
                       66.7
                   0.75
                   0.74
                16:13
                14:51
                                                              CONF_RPT  rev:3.02
  Houston Ship Channel

  36 oysters

-------
i i.   10.  o a
               u i
i O  r ivi
                                                                               J.  J. i
                            TRIANGLE LABORATORIES, INC.
                          CONFIRMATION ANALYSIS  (DB-225)
                                                                   Page 1 of 1
                                                                      11/13/89
FILE NAME..,,:  T894689     CLIENT ID....:  RTI         TLI NUMBER....:  22-15-10
CONCAL	:  T894678
ANALYST	:  MV
SAMPLE SIZE..:    20.51 g
ICAL DATE..,.:  08/18/80
SPIKE FH-E:;::.?^!)^!!....SB.«aB.ss.:SBBBS=BB.«.--«BBB===.»««=SS«6BB««BBB
^ME====r======:::1SCONC(pptrNUMBER	DL"""EMPC     RATIO	RT^
                              CLIENT  ID....: RTI
                              SAMPLE  ID.,,.: DF0253-01
                              ANALYSIS  DATE: 10/20/89
                              SAMPLE  MATRIX: FISH
                              SAMPLE  ORIGIN: n/a
                                 DATE COLLECTED:   /  /
                                 SHIPMENT NO,..: n/a
                                                                           FLAGS
2378-TCDD
2378-TCDF

TOTAL TCDD

TOTAL TCDF
ND
 0.14

ND

 0.14
                                          0.03


                                          0.03
0.85   16:14    JL
                                                            0.85
    SURROGATE STD.

    NAME
    37CL-TCDD
                                       : = ,__s!..s = = = sS = = = = S5SS = = = = SBBS = = = = a = S =

                 'cONc'(ppt)""       X  REC"_    RATIO	™_	FLAGS^
                                                           ^   ^^     	
                          58.0
                                       59.5
INTERNAL STANDARDS RECOVERY SUMMARY 	 	 __ 	
NAME
13C12-2378-TCDF
13C12-2378-TCDD
CONC (ppt)
71.4
51.9
% REC.
73.2
53.2
RATIO
0.73
0.76
RT
16:13
14:50
FLAGS
— —
                                                                  CONF_RPT  rev:3.02
          Lake Sam Rayburn

          2 freshwater drum (fillet)

-------
i i.   10.
u l :  i 0  rivi
                                  *TKiANtiLb   LAbb
                         TRIANGLE LABORATORIES, INC.
                       CONFIRMATION ANALYSIS (DB-5)
FILE NAME ---- :
CONCAL ....... :
ANALYST ...... :
SAMPLE SIZE..:
ICAL DATE....:
SPIKE FILE...:
                  T804963
                  T694958
                  PMC
                    20.20 g
                  08/21/89
                  SPCONF2K
CLIENT JO	  RTI
SAMPLE ID....;  DF0253-02
ANALYSIS DATE:  10/31/89
SAMPLE MATRIX:  FISH
SAMPLE ORIGIN:  n/a
                                                    Page  1  of  1
                                                       11/13/89

                                      TLI NUMBER....:  22-15-11

                                      PROJECT NUMBER:  14418
                                      DATE RECEIVED.:  09/06/89
                                      DATE COLLECTED:   /  /
                                      SHIPMENT NO...:  n/a
NAME
CONC(ppt) NUMBER      DL    EMPC
                                       RATIO
                                                                    RT
                                            FLAGS
2378-TCDD
2378-TCDF

TOTAL TCDD

TOTAL TCDF
     EMPC
       0.40

     EMPC

       0.49
                                                 0.30


                                                 0.30

                                                 0.55
                             0.72   20:07
                             0.80
SURROGATE STD. RECOVERY SUMMARY (TYPE B)

NAME             CONC (ppt)         % REC.       RATIO          RT     FLAGS
37CL-TCDD
      60.4
                                         61.0
                                  20:42
INTERNAL STANDARDS RECOVERY SUMMARY

NAME             CONC (ppt)         X REC.
                                RATIO
                                                                  RT
                                           FLAGS
13C12-2378-TCDF       88.1
13C12-2378-TCDD       51.4
                      89.0
                      51.9
                                                    0.78
                                                    0.80
                                  20:06
                                  20:40
 Lake Sam Rayburn

 2 crappie (fillet)
                                                             CONF_RPT  rev:3,02

-------
i .  _ 1 U.  Q
                            J. ivi
                                    '|s .1  I\ 1
                                               VJ ij C,   u n u »j
                                                                             J-  1
                          TRIANGLE LABORATORIES, INC.
                        CONFIRMATION ANALYSIS (DB-225)
 FILE NAME,...: T894688
 CONCAL	: T894678
 ANALYST	: MV
 SAMPLE SIZE..:   20.20 g
 ICAL DATE....: 08/18/89
 SPIKE FILE...: SPCONF2K
     CLIENT ID....; RTI
     SAMPLE ID,...: DF0253-02
     ANALYSIS DATE: 10/20/89
     SAMPLE MATRIX: FISH
     SAMPLE ORIGIN: n/a
                              Page  1  of  1
                                 11/13/89

                TLI NUMBER....:  22-1B-11

                PROJECT NUMBER:  14418
                DATE RECEIVED.:  09/06/89
                DATE COLLECTED:    /  /
                SHIPMENT NO...:  n/a
NAME
2378-TCDD
2378-TCDF
TOTAL TCDD
TOTAL TCDF
CONC (ppt)
EMPC
0.39
EMPC
0.47
NUMBER DL EMPC
0,22
0.22
2
RATIO
0.71

0,79
RT
16:15


FLAGS
JL
-

 SURROGATE STD.  RECOVERY SUMMARY (TYPE B)

 NAME             CONC (ppt)         % REC.      RATIO
                                   =====s!ss====ss===r:
                                         RT     FLAGS
 37CL-TCDD
71.4
72.1
14:52
 INTERNAL STANDARDS RECOVERY SUMMARY

 NAME             CONC (ppt)         X REC.
                          RATIO
                         RT
         FLAGS
 13C12-2378-TCDF       91.3
 13C12-2378-TCDD       57.5
                92.2
                58.1
           0.74
           0.76
16:13
14:51
       Lake Sam Rayburn

       2 crappie (fillet)
                                                              CONF_RPT rev:3.02

-------
                W X
                           J. J.Y1
                                   ''• 1 1\ 1 rt H U Li C,
                                                          r o u
                          TRIANGLE LABORATORIES,  INC.
                        CONFIRMATION  ANALYSIS (DB-5)
 FILE  NAME....: T894964
 CONCAL	: T894958
 ANALYST	: PMC
 SAMPLE SIZE..:   20.36 g
 ICAL  DATE....: 08/21/89
 SPIKE FILE,..: SPCONF2K
         CLIENT ID,,..: RTI
         SAMPLE ID....: OF0253-03
         ANALYSIS DATE: 10/31/89
         SAMPLE MATRIX: FISH
         SAMPLE ORIGIN: n/a
                                    Page 1 of  1
                                       11/13/89

                       TLI NUMBER....: 22-15-12

                       PROJECT NUMBER: U418
                       DATE RECEIVED.: 09/06/89
                       DATE COLLECTED:   /  /
                       SHIPMENT NO...: n/ft
NAME
CONC(ppt) NUMBER
         DL    EMPC
RATIO
         RT
FLAGS
2378-TCDD
2378-TCDF

TOTAL TCDD

TOTAL TCDF
  EMPC
    0.24

  EMPC

    0.24
               0.40
               0.40
1
                         0.79   20:07
0.79
SURROGATE STD. RECOVERY SUMMARY (TYPE B)

NAME             CONC (ppt)         X REC.

37CL-TCDD             65.5            66.7
                              RATIO
                                RT

                           •20:42
              FLAGS
INTERNAL STANDARDS RECOVERY SUMMARY

NAME             CONC (ppt)         % REC.      RATIO          RT     FLAGS
13C12-2378-TCDF
13C12-2378-TCDD
78.2
55.8
79.6
56.8
0.78
0.79
20:07
20:41 __
  Lake Sam Rayburn

  7 bluegill sunfish  (fillet)
                                                             CONF^RPT rev:3.02

-------
1 1 .   ID.  O
U 1
                                        1 I\ 1 rt 1H U
                            TRIANGLE LABORATORIES,  INC.
                          CONFIRMATION ANALYSIS  (DB-225)
FILE NAME	 T894698
CONCAL	: T894696
ANALYST	: MV
SAMPLE SIZE..:   20.36 g
ICAL DATE....: 08/18/89
SPIKE FILE...: SPCONF2K
SSS====S=2======52==	
                                                    Page 1 of 1
                                                       11/13/89

                                                   ..: 22-16-12
                              CLIENT  ID....: RTI         TLI  NUMBER.
                              SAMPLE  ID.,..: DF0253-03
                              ANALYSIS  DATE: 10/20/89    PROJECT  NUMBER:  14418
                              SAMPLE  MATRIX: FISH
                              SAMPLE  ORIGIN: n/fl
                                       DATE RECEIVED.: 09/06/89
                                       DATE COLLECTED:   /  /
                                       SHIPMENT NO...: n/8
                      CONC(ppt)  NUMBER      DL     EMPC     RATIO     RT    FLAGS
2378-TCDD
2378-TCDF
TOTAL TCDD
TOTAL TCDF
0.26
0.20
0.26
0.20

1
1

0.54
0.27
0.66 14:51 	
0.66 16:14 JL
0.66 	
0.66 	
    SURROGATE STD. RECOVERY_SUMMARY_(TYPEJ)	====S2S=====SSS2======S.S!:===
    =z====s========s=======s-=s======sss==i:	::::.          RT     FLAGS
NAME ' cc
37CL-TCDD
INTERNAL STANDARDS
NAME C(
13C12-2378-TCDF
13C12-2378-TCDD
>NC l.ppt;
80.5 •
RECOVERY
3NC (ppt)
79.5
62.8
7* nci»
82.0
SUMMARY
X REC.
80.9
63.9


RATIO
0.75
0.75

14:51 	
RT FLAGS
16:13 	
14:50 	
                                                                  CONF_RPT  rev:3.02
          Lake Sam Rayburn

          7 bluegill sunfish (fillet)

-------
11,   i
U 1 •  i  O
                                         I\ i
                                                VJ Lf li   u n u ts
                            TRIANGLE LABORATORIES, INC.
                          CONFIRMATION ANALYSIS (DB-B)
                                                    Page 1  of 1
                                                       11/13/89
FILE NAME...,: T894959 CLIENT ID....: RTI
CONCAL ..: T894958 SAMPLE ID....: DF0253-06
ANALYST 	 : PMC ANALYSIS DATE: 10/31/89
SAMPLE SIZE..: 20.06 g SAMPLE MATRIX: FISH
1CAL DATE....: 08/21/89 SAMPLE ORIGIN: n/a
SPIKE FILE...: SPCONF2K

NAME CONC (ppt) NUMBER DL EMPC
2378-TCDD 9.1
2378-TCDF 2.0
TOTAL TCDD 9.1 1
TOTAL TCDF 2.0 1
SURROGATE STD. RECOVERY SUMMARY (TYPE B)
NAME CONC (ppt) X REC. RATIO
37CL-TCDD 38.0 £j2^
INTERNAL STANDARDS RECOVERY SUMMARY
NAME CONC (ppt) X REC. RATIO
13C12-2378-TCDF 56.7 J$^ °'J?
13C12-2378-TCDD 32.8 £32.9y °'78
TLI NUMBER.,,.:

PROJECT NUMBER:
DATE RECEIVED.:
DATE COLLECTED:
SHIPMENT NO,.,:

RATIO RT
0.72 20:41
0.81 20:07
0.72
0.81

RT
20:42

RT
20:06
20:40
22-15-6

14418
09/06/89
/ /
n/a

FLAGS

JS_
—
	

FLAGS
_*L

FLAGS
_
y
                                                                 CONF RPT rev:3.02
    Neches River

    5 spotted gar (fillet)

-------
                    TRIANGLE LABORATORIES, INC.            Pa9!<1/«Sf,0J
                  CONFIRMATION ANALYSIS (OB-225)              11/13/B9

FILE NAME. ...'. T894693    CLIENT ID. . . . : RTI       TLI NUMBER. . . . :  22-15-6
CONCAL ...... : T894678    SAMPLE ID    : DF0253-06


EuEi ja/  =8 Si r       gi sss;  -rr
SPIKE FIU. . . , SPCO..F2K
                  .......................... «.
NAME""" ........ coiic(pptj NUMBER     DL    IHPC  __R*TIO ____ RT ____ FLAQS

                  j-i                        s-sli

TOTAL TCDD          8.7     1                   0.75

TOTAL TCOF          2.1     1                   °'77
SURROGATE STD.                   _  ....... =sss=====s=s=====s8fis====ass

            "'"""*"""         ""
                                      RATIO   _     _RT    FLAGS_


 37CL-TCDD          41.4          41.6               14:51    _




 INTERNAL STANDARDS RECOVERY SUMMARY  __	__	=-ss---===-----=s==ss:


 ISr""""""™^^	^AGS_


 TSC^^^TCD'F      sTo        J|^     *•"       JJ;JJ    -%
 13C12-2378-TCDD      33.3        S5*^>     0.11       ^-^    -X-
                                                 CONF_RPT rev:3.02
     Neches River


     5 spotted gar (fillet)

-------
1 .
         U C'
                          TRIANGLE  LABORATORIES, INC.
                        CONFIRMATION ANALYSIS  (DB-5)
FILE NAME ____ : T894956
CQNCAL ....... : T894942
ANALYST ...... : DM
SAMPLE SIZE..:   20.00 g
ICAL DATE ____ : 08/21/89
SPIKE FILE...: SPCONF2K

NAME ...........   CONC(ppt) NUMBER      DL    EMPC
                            CLIENT ID ---- : RTI
                            SAMPLE ID ---- : DF0253-05
                            ANALYSIS DATE: 10/31/89
                            SAMPLE MATRIX: FISH
                            SAMPLE ORIGIN: n/a
                                                                   Pago 1 of 1
                                                                      11/13/89

                                                      TLI NUMBER....: 22-15-5

                                                      PROJECT NUMBER: 14418
                                                      DATE RECEIVED,: 09/06/89
                                                      DATE COLLECTED:   /  /
                                                      SHIPMENT NO,,.: n/a

                                                       RATIO     RT    FLAGS
2378-TCDD
2378-TCDF

TOTAL TCDD

TOTAL TCDF
                      4.7
                      3.8

                      4.7

                      3.8
                                                         0.79   20:44    -
                                                         0.72   20:10    JL.

                                                         0.79            -

                                                         0.72
SURROGATE STD. RECOVERY SUMMARY (TYPE B)
==r=i:r====================== = = ====== = =====
NAME             CONC (ppt)         % REC.

37CL-TCOD             49.0            49.0
                                                RATIO
                                                               RT

                                                             20:44
                                                                       FLAGS
 INTERNAL STANDARDS RECOVERY SUMMARY
NAME
13C12-2378-TCDF
13C12-2378-TCDD
CONC (ppt)
82.7
48.6
X REC.
82.7
48.6
RATIO
0.79
0.78
RT
20:09
20:43
FLAGS
	
  Neches River

  5 largemouth bass  (fillet)

-------
1  .   1 5.  89     01:18   PM    *TRI ANGLE  LAbb
                            TRIANGLE LABORATORIES, INC.
                          CONFIRMATION ANALYSIS (DB-225)

        NAME      T894687     CLIENT ID.,,.: RTI         TLI NUMBER....: 22-15-5

            ::;   r4678     Si™ W20/8?5   PROJECT NUMBER: 14418
            ::;   "Von .   SA^MAT^x; F&        DAT| RECEIVED  : M/N/.I
    	   08/1JJ/B9    SAMPLE ORIGIN: n/a         ^COLLECTED.    //
    SPIKE  FILE..   SPCONF2K	fts=======ss£s=============«=====««====="«==
    --------- -             --                                 Oj7g    14;51
    2378-TCDD              J.|                               0<75    16:14
    2378-TCDF              4-3
                          4  q      <                         0.76
    TOTAL TCDD             ^«3      1

    TOTAL TCDF             4.3      1                         °'75
======SSSS=====
    SURROGATE STD. RECOVERY SUMMARY (TYPEJ)

    ^-^Z^=^^^"^^^~
    -------------------- -                                      14:51
    37CL-TCDD             59-1            59§1

    INTERNAL STANDARDS RECOVERY SUMMARY _____ ...... _____ ===sSSS-===sass
                                       "
                          STj            89.4       075         16:18
     13C12-2378-TCDD       54.3^  ^       ___ 5^3 _______ u^ _________________________
     _- -------------------------------                          CONF^RPT  rev: 3. 02


          Neches River

          5 largemouth bass (fillet)

-------
             NDS PHASE II:  BIOACCUMULATIVE POLLUTANT STUDY:
                      Sample Tracking System          ERL-D Loc,
                                                               1358
EPISODE #:  3452
                                      SCC #:  DF025219
 Sampling Information:
    Sampling Office:  AR
                     DEOT. POLLUTION CONTROL AND ECOLOGY
                                    AR
City and State:  LITTLE ROCK
Sampling Contact:  JOHN GIESE
Site Location:  AR RED RIVER AT INDEX
Latitude:  N 34 15' 0"
Date Sampled:   9/12/89
Matrix Type:  F PF BLUE CATFISH
Sample Composite:  06
Lt:(Range in CM,LOW,HIGH)     43.00
Wt:(Range in G,LOW,HIGH)     817.00
Pulp&Paper Flag:
                                                     Region:
                                          Longitude:  W
                                          Date Received:
                94  6' 0"
                  9/19/89
                                          Species code:  6C
                                                    55.00
                                                  1725.00
                                          Hg Completed:
Analytical:
  Extraction Date:
         GC/MS ID:
           LAB ID:
           Weight:
           %Lipid:
   DPE Indication:
                PCDD/PCDF
                10/31/89
                MAT891473
                C103189CS
                20.11
                 3.0
Pesticide & Industrial Chemicals
          O/ O/ 0
          0.00
          0.0
                           Mass lipid on GPC:  0.000
  Comments:
Xenobiotic Definitions:
   QA FLAGS:
       E - exceeds highest calibration standard
       D - below limit of quantitation

   Limits of  quantitation:
       Pesticides - 2.5  ppb
       PCBs:   1-3 chloro  -  1.25 ppb
              4-6 chloro  -  2.50 ppb
              7-8 chloro  -  3.75 ppb
              9-10 chloro -  6.25 ppb

-------
            NDS PHASE II:  BIOACCUMULATTVE POLLUTANT STUDY
EPISODE ft:  3452
         SCC ft:  DF025219
                   ERL-D Loc.:  1358
DATA for BIOSIGNIFICANT POLYCHLORINATED DIBENZODIOXINS AND FURANS:
Analyte               CAS No.:    Ion Ratio   S/N  2REC Det.  Lim.   Amount(pgXg)
2,3,7,8- TCDF
2,3,6,7- TCDF
3,4,6,7- TCDF
51207-31-9
0.73
0.00
0.00
25.16
 1.00
 1.00
65
65
65
0.0000
0.7700
0.7700
 10.98
ND
ND
2,3,7,8- TCDD
 1746-01-6   0.75    214.78   78
                        0.0000
                         40.73
1,2,3,7,8- PeCDF
2,3,4,7,8- PeCDF
2,3,4,6,7- PeCDF
57117-41-6   0.00      1.00   89     0.8000   ND
57117-31-6   2.10      1.00   89     1.7800   ND
70648-29-9   0.00      1.00   89     0.8400   ND
1,2,3,7,8- PeCDD
40321-76-4   0.70
          5.24   73
               0.0000
                  1.06
1,2,3,4,6,7- HxCDF *
1,2,3,4,7,8- HxCDF
1,2,3,6,7,8- HxCDF
2,3,4,6,7,8- HxCDF
1,2,3,7,8,9- HxCDF
1,2,3,4,7,8- HxCDD
1,2,3,6,7,8- HxCDD
1,2,3,7,8,9- HxCDD
1,2,3,4,6,7,8- HpCDF
1,2,3,4,7,8,9- HpCDF
19408-74-3
70648-26-9
57117-44-9
60851-34-5
72918-21-9
32598-13-3
57753-85-7
19408-74-3
67562-39-4
55673-89-7

0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00

1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
,
85
85
85
85
76
76
76
52
52

2.8200
2.8300
1.9500
2.7600
2.7100
2.0300
1.5400
1.4900
2.6800

ND
ND
ND
ND
ND
ND
ND
ND
ND
1,2,3,4,6,7,8- HpCDD   37871-00-4   0.00      1.00   59
*:  Coelutes with 123478 HxCDF on DBS 30 H.
                                     1.9900   ND
        Toxicity Equivalency Concentration:    42.36
        Percent contribution of 2378 TCDF,
        2378 TCDD and 12378 PeCDD to total
        TEF value:                               100

-------
             NDS PHASE II:  BIOACCUMULATIVE POLLUTANT STUDY:
                      Sample Tracking System          ERL-D Loc.:  1359
EPISODE ft:  3452
                                         SCC ft:   DF025220
                                                     Region:
Sampling Information:
   Sampling Office:  AR DEPT.  POLLUTION CONTROL AND ECOLOTY
   City and State:  LITTLE ROCK    AR
   Sampling Contact:  JOHN GIESE
   Site Location:  AR RED RIVER AT INDEX
   Latitude:  N 34 15' 0"
   Date Sampled:   9/12/89
   Matrix Type:  F PF FLATHEAD CATFISH
   Sample Composite:  02
   Lt:(Range in CM,LOW,HIGH)      52.00
   Wt:(Range in G,LOW,HIGH)    1453.00
   Pulp&Paper Flag:
                                          Longitude:  W
                                          Date Received:
                94  6'  0"
                  9/19/89
                                          Species code:  6B
                                                    61.00
                                                  2361.00
                                          Hg Completed:
Analytical:
  Extraction Date:
         GC/MS ID:
           LAB ID:
           Weight:
           XLipid:
   DPE Indication:
                   PCDD/PCDF
                   10/31/89
                   MAT891474
                   D103189CS
                   20.02
                    1.2
Pesticide & Industrial Chemicals
          O/ O/ 0
          0.00
          0.0
                              Mass lipid on GPC:   0.000
  Comments:
Xenobiotic Definitions:
   QA FLAGS:
       E - exceeds highest calibration standard
       D - below limit of quantitation

   Limits of quantitation:
       Pesticides - 2.5 ppb
       PCBs:  1-3 chloro  -  1.25 ppb
              4-6 chloro  -  2.50 ppb
              7-8 chloro  -  3.75 ppb
              9-10 chloro -  6.25 ppb

-------
            NDS PHASE II:  BIOACCUMULATIVE POLLUTANT STUDY
EPISODE #:  3452
         SCC #:  DF025220
                   ERL-D Loc.:  1359
DATA for BIOSIGNIFICANT POLYCHLORINATED DIBENZODIOXINS AND FURANS:
Analyte               CAS No.:   Ion Ratio   S/N  £REC Det. Lim.  Amount(pg/g)
2,3,7,8- TCDF
2,3,6,7- TCDF
3,4,6,7- TCDF
51207-31-9
0.00
0.00
0.00
1.00   62
1.00   62
1.00   62
       0.5400   ND
       0.5400   ND
       0.5400   ND
2,3,7,8- TCDD
 1746-01-6   0.81
          7.75   83
              0.0000
                  2.26
1,2,3,7,8- PeCDF
2,3,4,7,8- PeCDF
2,3,4,6,7- PeCDF
57117-41-6
57117-31-6
70648-29-9
0.00
0.00
0.00
1.00
1.00
1.00
88
88
88
0.8000
0.8600
0.8400
ND
ND
ND
1,2,3,7,8- PeCDD
40321-76-4   0.00
          1.00   83
              1.1600   ND
1,2,3,4,6,7- HxCDF *
1,2,3,4,7,8- HxCDF
1,2,3,6,7,8- HxCDF
2,3,4,6,7,8- HxCDF
1,2,3,7,8,9- HxCDF
1,2,3,4,7,8- HxCDD
1,2,3,6,7,8- HxCDD
1,2,3,7,8,9- HxCDD
1,2,3,4,6,7,8- HpCDF
1,2,3,4,7,8,9- HpCDF
19408-74-3
70648-26-9
57117-44-9
60851-34-5
72918-21-9
32598-13-3
57753-85-7
19408-74-3
67562-39-4
55673-89-7

0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00

1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00

90
90
90
90
85
85
85
68
68

2.8300
2.8400
1.9600
2.7700
2.4700
1.8400
1.3800
1.4500
2.6100

ND
ND
ND
ND
ND
ND
ND
ND
ND
1,2,3,4,6,7,8- HpCDD   37871-00-4   0.00
*:  Coelutes with 123478 HxCDF on DBS 30 M.
                       1.00   72
                        1.2600   ND
        Toxicity Equivalency Concentration:     2.26
        Percent contribution of 2378 TCDF,
        2378 TCDD and 12378 PeCDD to total
        TEF value:                               100

-------
             NDS PHASE II:  BIOACCUMULATIVE POLLUTANT STUDY:
                      Sample Tracking System          ERL-D Loc,
                                                                  1357
EPISODE #:  3452
                                         SCC ft:   DF025218
                                                     Region:
Sampling Information:
   Sampling Office:   AR DEPT.  POLLUTION CONTROL AND ECOLOGY
   City and State:   LITTLE ROCK    AR
   Sampling Contact:   JOHN GIESE
   Site Location:   AR RED RIVER AT INDEX AR
   Latitude:   N 34  15'  0"
   Date Sampled:    9/12/89
   Matrix Type:  F  WB S.M. BUFFALO
   Sample Composite:   06
   Lt:(Range in CM,LOW,HIGH)     40.00
   Vt:(Range in G,LOW,HIGH)    1135.00
   Pulp&Paper Flag:
                                          Longitude:  W
                                          Date Received:
                94  6' 0"
                  9/19/89
                                          Species code:  9A
                                                    54.00
                                                  2860.00
                                          Hg Completed:
Analytical:
  Extraction Date:
         GC/HS ID:
           LAB ID:
           Weight:
           %Lipid:
   DPE Indication:
                   PCDD/PCDF
                   10/31/89
                   MAT891471
                   A103189CS
                   20.04
                    2.2
Pesticide & Industrial Chemicals
          O/ O/ 0
                              Mass lipid on GPC:
          0.00
          0.0
          0.000
  Comments:  PCDD/PCDF:  DUPLICATE IS QD103189
Xenobiotic Definitions:
   QA FLAGS:
       E - exceeds highest calibration standard
       D - below limit of quantitation

   Limits of  quantitation:
       Pesticides - 2.5  ppb
       PCBs:   1-3 chloro  -  1.25 ppb
              4-6 chloro  -  2.50 ppb
              7-8 chloro  -  3.75 ppb
              9-10 chloro -  6.25 ppb

-------
            NDS PHASE II:  BIOACCUMULATIVE POLLUTANT STUDY
EPISODE #:  3452
         SCC t:  DF025218
                   ERL-D Loc.:  1357
DATA for BIOSIGNIFICANT POLYCHLORINATED DIBENZODIOXINS AND FURANS:
Analyte               CAS No.:   Ion Ratio   S/N  %REC Det. Lim.  Amount(pgXg)
2,3,7,8- TCDF
2,3,6,7- TCDF
3,4,6,7- TCDF
51207-31-9   0.73     73.29   73     0.0000    17.37
             0.00      1.00   73     0.5800   ND
             0.00      1.00   73     0.5800   ND
2,3,7,8- TCDD
 1746-01-6   0.76
         50.68   91
              0.0000
                  9.01
1,2,3,7,8- PeCDF
2,3,4,7,8- PeCDF
2,3,4,6,7- PeCDF
57117-41-6
57117-31-6
70648-29-9
0.00
0.00
0.00
1.00  102
1.00  102
1.00  102
       0.9300
       0.9600
       0.9500
         ND
         ND
         ND
1,2,3,7,8- PeCDD
40321-76-4   0.00
          1.00   86
              1.1000   ND
1,2,3,4,6,7- HxCDF *
1,2,3,4,7,8- HxCDF
1,2,3,6,7,8- HxCDF
2,3,4,6,7,8- HxCDF
1,2,3,7,8,9- HxCDF
19408-74-3
70648-26-9
57117-44-9
60851-34-5
72918-21-9

0.00
0.00
0.00
0.00

1.00
1.00
1.00
1.00

99
99
99
99

2.8300
2.8400
1.9600
2.7700

ND
ND
ND
ND
1,2,3,4,7,8- HxCDD
1,2,3,6,7,8- HxCDD
1,2,3,7,8,9- HxCDD
32598-13-3   0.00      1.00   83
57753-85-7   0.00      1.00   83
19408-74-3   0.00      1.00   83
                        2.4600   ND
                        1.8400   ND
                        1.3800   ND
1,2,3,4,6,7,8- HpCDF
1,2,3,4,7,8,9- HpCDF
67562-39-4
55673-89-7
0.00
0.00
1.00
1.00
71
71
1.4400
2.6100
ND
ND
1,2,3,4,6,7,8- HpCDD   37871-00-4   0.00      1.00   77
*:  Coelutes with 123478 HxCDF on DBS 30 M.
                                     1.3900   ND
        Toxicity Equivalency Concentration:     10.75
        Percent contribution of 2378 TCDF,
        2378 TCDD and 12378 PeCDD to total
        TEF value:                               100

-------
             NDS PHASE II:  BIOACCUMULATIVE POLLUTANT STUDY:
                      Sample Tracking System          ERL-D Loc.
EPISODE ft:  3452
                                                                  1355
                                         SCC ft:  QD103189
                                                     Region:
Sampling Information:
   Sampling Office:   AR DEPT.  POLLUTION CONTROL AND ECOLOGY
   City and State:   LITTLE ROCK    AR
   Sampling Contact:  JOHN GIESE
   Site Location:   AR RED RIVER AT INDEX AR
   Latitude:  N 34  15' 0"
   Date Sampled:    9/12/89
   Matrix Type:   F  WB SM BUFFALO
   Sample Composite:  06
   Lt:(Range in CM,LOW,HIGH)      40.00
   Vt:(Range in G,LOU,HIGH)    1135.00
   Pulp&Paper Flag:
                                          Longitude:  V
                                          Date Received:
                94  6' 0"
                  9/19/89
                                          Species code:  9A
                                                    54.00
                                                  2860.00
                                          Hg Completed:
Analytical:
  Extraction Date:
         GC/MS ID:
           LAB ID:
           Weight:
           %Lipid:
   DPE Indication:
                   PCDD/PCDF
                   10/31/89
                   MAT891472
                   B103189CS
                   20.02
                    2.4
Pesticide & Industrial Chemicals
          O/ O/ 0
                              Mass lipid on GPC:
          0.00
          0.0
          0.000
  Comments:  PCDD/PCDF: DUPLICATE OF DF025218
Xenobiotic Definitions:
   QA FLAGS:
       E - exceeds highest calibration standard
       D - belov limit of quantitation

   Limits of  quantitation:
       Pesticides - 2.5 ppb
       PCBs:   1-3 chloro  -  1.25 ppb
              4-6 chloro  -  2.50 ppb
              7-8 chloro  -  3.75 ppb
              9-10 chloro -  6.25 ppb

-------
            NDS PHASE II:  BIOACCUMULATIVE POLLUTANT STUDY
EPISODE #:  3452
         SCC #:  QD103189
                   ERL-D Loc.:  1355
DATA for BIOSIGNIFICANT POLYCHLORINATED DIBENZODIOXINS AND FURANS:
Analyte               CAS No.:   Ion Ratio   S/N  %REC Det. Lim.  Amount(pg/g)
2,3,7,8- TCDF
2,3,6,7- TCDF
3,4,6,7- TCDF
51207-31-9
0.73
0.00
0.00
41.83
 1.00
 1.00
68
68
68
0.0000
0.7400
0.7400
 16.85
ND
ND
2,3,7,8- TCDD
 1746-01-6   0.71
         48.25   84
               0.0000
                  9.03
1,2,3,7,8- PeCDF
2,3,4,7,8- PeCDF
2,3,4,6,7- PeCDF
57117-41-6
57117-31-6
70648-29-9
0.00
0.00
0.00
 1.00
 1.00
 1.00
94
94
94
0.8000
0.8700
0.8500
ND
ND
ND
1,2,3,7,8- PeCDD
40321-76-4   0.00
          1.00   83
               1.1000   ND
1,2,3,4,6,7- HxCDF *
1,2,3,4,7,8- HxCDF
1,2,3,6,7,8- HxCDF
2,3,4,6,7,8- HxCDF
1,2,3,7,8,9- HxCDF
1,2,3,4,7,8- HxCDD
1,2,3,6,7,8- HxCDD
1,2,3,7,8,9- HxCDD
1,2,3,4,6,7,8- HpCDF
1,2,3,4,7,8,9- HpCDF
19408-74-3
70648-26-9
57117-44-9
60851-34-5
72918-21-9
32598-13-3
57753-85-7
19408-74-3
67562-39-4
55673-89-7

0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00

1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
.
93
93
93
93
84
84
84
70
70

2.8300
2.8400
1.9600
2.7700
2.4700
1.8400
1.3800
1.4500
2.6100

ND
ND
ND
ND
ND
ND
ND
ND
ND
1,2,3,4,6,7,8- HpCDD   37871-00-4   0.00
*:  Coelutes with 123478 HxCDF on DBS 30 M.
                       1.00   66
                        1.2900   ND
        Toxicity Equivalency Concentration:     10.72
        Percent contribution of 2378 TCDF,
        2378 TCDD and 12378 PeCDD to total
        TEF value:                               100

-------
             NDS PHASE II:  BIOACCUMULATIVE POLLUTANT STUDY:
                      Sample Tracking System          ERL-D Loc.:  1006

EPISODE ft:  3418                          SCC ft:  DF025007
 Sampling Information:
    Sampling Office:  OFFICE WAER RESOURCES
    City and State:  BATON ROUGE    LA
    Sampling Contact:  RONNIE ALBRITTON              Region:   6
    Site Location:  LA MISSISSIPPI RIVER AT ZACHORY
   . Latitude:  N 30 39' 0"                Longitude:  V  91 17' 0"
    Date Sampled:   5/16/89               Date Received:   5/23/89
    Matrix Type:  F PF BLUE CATFISH
    Sample Composite:  08                 Species code:  6C
    Lt:(Range in CM,LOW,HIGH)     26.70             34.30
    Wt:(Range in G,LOW,HIGH)     170.00            396.00
    Pulp&Paper Flag:  Y                   Hg Completed:  N
Analytical:         PCDD/PCDF            Pesticide & Industrial Chemicals
  Extraction Date:   8/10/89                       O/ O/ 0
         GC/MS ID:  MAT891184
           LAB ID:  C081089CS
           Weight:  20.03                          0.00
           %Lipid:   2.0                           0.0
   DPE Indication:             Mass lipid on GPC:  0.000
  Comments:
Xenobiotic Definitions:
   QA FLAGS:
       E - exceeds highest calibration standard
       D - below limit of quantitation

   Limits of quantitation:
       Pesticides - 2,.5 ppb
       PCBs:  1-3 chloro  -  1.25 ppb
              4-6 chloro  -  2.50 ppb
              7-8 chloro  -  3.75 ppb
              9-10 chloro -  6.25 ppb

-------
            NDS PHASE II:  BIOACCUMULATIVE POLLUTANT STUDY
EPISODE ft:  3418
         SCC #:  DF025007
                   ERL-D Loc.:  1006
DATA for BIOSIGNIFICANT POLYCHLORINATED DIBENZODIOXINS AND FURANS:
Analyte               CAS No.:   Ion Ratio   S/N  XREC Det. Lim.  Amount(pg/g)
2,3,7,8- TCDF
2,3,6,7- TCDF
3,4,6,7- TCDF
51207-31-9
0.88
0.00
0.00
6.34   92
1.00   92
1.00   92
0.0000
0.4900
0.4900
  0.65
                                              ND
                                              ND
2,3,7,8- TCDD
 1746-01-6   0.76
         10.72   95
              0.0000
           1.40
1,2,3,7,8- PeCDF
2,3,4,7,8- PeCDF
2,3,4,6,7- PeCDF
57117-41-6
57117-31-6
70648-29-9
0.00
1.57
0.00
1.00   94
1.00   94
1.00   94
0.7700
1.1200
0.8200
ND
ND
ND
1,2,3,7,8- PeCDD
40321-76-4   0.00
          1.00  113
              1.0100   ND
1,2,3,4,6,7- HxCDF *
1,2,3,4,7,8- HxCDF
1,2,3,6,7,8- HxCDF
2,3,4,6,7,8- HxCDF
1,2,3,7,8,9- HxCDF
1,2,3,4,7,8- HxCDD
1,2,3,6,7,8- HxCDD
1,2,3,7,8,9- HxCDD
1,2,3,4,6,7,8- HpCDF
1,2,3,4,7,8,9- HpCDF
19408-74-3
70648-26-9
57117-44-9
60851-34-5
72918-21-9
32598-13-3
57753-85-7
19408-74-3
67562-39-4
55673-89-7

0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00

1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00

90
90
90
90
97
97
97
74
74

2.8300
2.8400
1.9600
2.7700
2.4600
1.8400
1.3800
1.4500
2.6100

ND
ND
ND
ND
ND
ND
ND
ND
ND
1,2,3,4,6,7,8- HpCDD   37871-00-4   0.82
*:  Coelutes with 123478 HxCDF on DBS 30 H.
                       1.00   85
                        1.5200   ND
        Toxicity Equivalency Concentration:      1.47
        Percent contribution of 2378 TCDF,
        2378 TCDD and 12378 PeCDD to total
        TEF value:                               100

-------
             NDS PHASE II:  BIOACCUMULATTVE POLLUTANT STUDY:
                      Sample Tracking System          ERL-D Loc.
EPISODE ft:  3416
                                      SCC #:  DF025211
                                                               1377
 Sampling Information:
    Sampling Office:  LA DEFT. OF ENV QUALITY
    City and State:  MONROE         LA
    Sampling Contact:  KIRK CORMIER                  Region:
                    LA OUACHITA RIVER NORTH OF STERLINYTON
Site Location:
Latitude:  N 33  0' 0"
Date Sampled:   9/ 6/89
Matrix Type:  F BF CARP
Sample Composite:  03
Lt:(Range in CM,LOW,HIGH)
Wt:(Range in G,LOW,HIGH)
Pulp&Paper Flag:
                                  51.00
                                1769.00
                                          Longitude:  W
                                          Date Received:
                92  4' 0"
                  9/27/89
 Species code:  1
           57.50
         2540.00
 Hg Completed:
Analytical:
  Extraction Date:
         GC/MS ID:
           LAB ID:
           Weight:
           %Lipid:
   DPE Indication:
                PCDD/PCDF
                10/31/89
                MAT891478
                G103189CS
                20.02
                 2.0
Pesticide & Industrial Chemicals
          O/ O/ 0
          0.00
          0.0
                           Mass lipid on GPC:  0.000
  Comments:
Xenobiotic Definitions:
   QA FLAGS:
       E - exceeds highest calibration standard
       D - below limit of quantitation

   Limits of quantitation:
       Pesticides - 2.5 ppb
       PCBs:  1-3 chloro  -  1.25 ppb
              4-6 chloro  -  2.50 ppb
              7-8 chloro  -  3.75 ppb
              9-10 chloro -  6.25 ppb

-------
            NDS PHASE II:  BIOACCUMULATIVE POLLUTANT STUDY
EPISODE #:  3416
         SCC ft:  DF025211
                   ERL-D Loc.:  1377
DATA for BIOSIGNIFICANT POLYCHLORINATED DIBENZODIOXINS AND FURANS:
Analyte               CAS No.:   Ion Ratio   S/N  2REC Det. Lim.  Amount(pgXg)
2,3,7,8- TCDF
2,3,6,7- TCDF
3,4,6,7- TCDF
51207-31-9
0.76
0.00
0.00
17.87
 1.00
 1.00
69
69
69
0.0000
0.5100   ND
0.5100   ND
2.97
2,3,7,8- TCDD
 1746-01-6   0.77
         33.47   91
               0.0000
                  6.46
1,2,3,7,8- PeCDF
2,3,4,7,8- PeCDF
2,3,4,6,7- PeCDF
57117-41-6   0.00      1.00   86     0.7700   ND
57117-31-6   1.52      1.00   86     1.2400   ND
70648-29-9   0.00      1.00   86     0.8200   ND
1,2,3,7,8- PeCDD
40321-76-4   0.87
          1.00   85
               1.3700   ND
1,2,3,4,6,7- HxCDF *
1,2,3,4,7,8- HxCDF
1,2,3,6,7,8- HxCDF
2,3,4,6,7,8- HxCDF
1,2,3,7,8,9- HxCDF
1,2,3,4,7,8- HxCDD
1,2,3,6,7,8- HxCDD
1,2,3,7,8,9- HxCDD
1,2,3,4,6,7,8- HpCDF
1,2,3,4,7,8,9- HpCDF
19408-74-3
70648-26-9
57117-44-9
60851-34-5
72918-21-9
32598-13-3
57753-85-7
19408-74-3
67562-39-4
55673-89-7

0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00

1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00

84
84
84
84
79
79
79
56
56

2.8300
2.8400
1.9600
2.7700
2.4700
1.8400
1.3800
1.4500
2.6100

ND
ND
ND
ND
ND
ND
ND
ND
ND
1,2,3,4,6,7,8- HpCDD   37871-00-4   1.02
*:  Coelutes with 123478 HxCDF on DBS 30 H.
                       6.90   53
                        0.0000
                          2.61
        Toxicity Equivalency Concentration:     6.78
        Percent contribution of 2378 TCDF,
        2378 TCDD and 12378 PeCDD to total
        TEF value:                               100

-------
             NDS PHASE II:  BIOACCUMULATIVE POLLUTANT STUDY:
                      Sample Tracking System          ERL-D Loc.:  1376
EPISODE #:  3416
                      SCC ft:  DF025210
 Sampling Information:
    Sampling Office:  LA DEPT OF ENV QUALITY
    City and State:  MONROE         LA
    Sampling Contact:  KIRK CORMIER                  Region:
    Site Location:  LA OUACHITA R. NORTH OF STERLINYTON
    Latitude:  N 33  0' 0"
    Date Sampled:   9/ 6/89
    Matrix Type:  F PF CHANNEL CATFISH
    Sample Composite:  03
    Lt:(Range in CM,LOW,HIGH)     37.00
    Wt:(Range in G,LOW,HIGH)     453.00
    Pulp&Paper Flag:
                      Longitude:  W
                      Date Received:
                92  4' 0"
                  9/27/89
                      Species code:  6A
                                43.50
                               635.00
                      Hg Completed:
Analytical:
  Extraction Date:
         GC/MS ID:
           LAB ID:
           Weight:
           "'ipid:
   DPE Indication:
PCDD/PCDF
10/31/89
MAT891477
F103189CS
20.09
 3.1
Pesticide & Industrial Chemicals
          O/ O/ 0
          0.00
          0.0
           Mass lipid on GPC:  0.000
  Comments:
Xenobiotic Definitions:
   QA FLAGS:
       E - exceeds highest calibration standard
       D - below limit of quantitation

   Limits of quantitation:
       Pesticides - 2.5 ppb
       PCBs:  1-3 chloro  -  1.25 ppb
              4-6 chloro  -  2.50 ppb
              7-8 chloro  -  3.75 ppb
              9-10 chloro -  6.25 ppb

-------
            NDS PHASE II:  BIOACCUHULATIVE POLLUTANT STUDY
EPISODE #:  3416
         SCC #:  DF025210
                   ERL-D Loc.:  1376
DATA for BIOSIGNIFICANT POLYCHLORINATED DIBENZODIOXINS AND FURANS:
Analyte               CAS No.:    Ion Ratio   S/N  %REC Det. Lira.  Amount(pg/g)
2,3,7,8- TCDF
2,3,6,7- TCDF
3,4,6,7- TCDF
51207-31-9
0.74
0.00
0.00
9.51
1.00
1.00
66
66
66
0.0000
0.5200
0.5200
  1.68
                                              ND
                                              ND
2,3,7,8- TCDD
 1746-01-6   0.81
         31.46   90
              0.0000
                  3.14
1,2,3,7,8- PeCDF
2,3,4,7,8- PeCDF
2,3,4,6,7- PeCDF
57117-41-6
57117-31-6
70648-29-9
0.00
1.15
0.00
1.00
1.00
1.00
80
80
80
0.8200
0.9400
0.8600
ND
ND
ND
1,2,3,7,8- PeCDD
40321-76-4   0.64
          1.00   83
              1.1700   ND
1,2,3,4,6,7- HxCDF *
1,2,3,4,7,8- HxCDF
1,2,3,6,7,8- HxCDF
2,3,4,6,7,8- HxCDF
1,2,3,7,8,9- HxCDF
1,2,3,4,7,8- HxCDD
1,2,3,6,7,8- HxCDD
1,2,3,7,8,9- HxCDD
1,2,3,4,6,7,8- HpCDF
1,2,3,4,7,8,9- HpCDF
19408-74-3
70648-26-9
57117-44-9
60851-34-5
72918-21-9
32598-13-3
57753-85-7
19408-74-3
67562-39-4
55673-89-7

0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00

1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00

80
80
80
80
77
77
77
51
51

2.8200
2.8300
1.9500
2.7600
2.4600
1.8400
1.3700
1.4400
2.6100

ND
ND
ND
ND
ND
ND
ND
ND
ND
1,2,3,4,6,7,8- HpCDD   37871-00-4   0.89      5.10   52     0.0000
*:  Coelutes vith 123478 HxCDF on DBS 30 M.
                                                2.19
        Toxicity Equivalency Concentration:      3.33
        Percent contribution of 2378 TCDF,
        2378 TCDD and 12378 PeCDD to total
        TEF value:                                99

-------
             NDS PHASE II:  BIOACCUMULATIVE POLLUTANT STUDY:
                      Sample Tracking System          ERL-D Loc.:  1375
EPISODE #:  3416
                      SCC ft:  DF025212
 Sampling Information:
    Sampling Office:  LA. DEPT OF ENV QUALITY
    City and State:  MONROE         LA
    Sampling Contact:  KIRK CORMIER                  Region:
    Site Location:  LA OUACHITA RIVER NORTH OF STERLINYTON
    Latitude:  N 33  0' 0"
    Date Sampled:   9/ 6/89
    Matrix Type:  F PF L.M. BASS
    Sample Composite:  03
    Lt:(Range in CM,LOW,HIGH)
    Wt:(Range in G,LOW,HIGH)
    Pulp&Paper Flag:
              30.50
               0.00
 Longitude:  W  92  A' 0"
 Date Received:   9/37/89

 Species code:  8B
           37.50
            0.00
 Hg Completed:
Analytical:
  Extraction Date:
         GC/MS ID:
           LAB ID:
           Weight:
           %Lipid:
   DPE Indication:
PCDD/PCDF
10/31/89
MAT89U75
E103189CS
20.06
 1.6
Pesticide £. Industrial Chemicals
          O/ O/ 0
          0.00
          0.0
           Mass lipid on GPC:  0.000
  Comments:  NO WEIGHTS GIVEN
Xenobiotic Definitions:
   QA FLAGS:
       E - exceeds highest calibration standard
       D - below limit of quantitation

   Limits of  quantitation:
       Pesticides - 2.5 ppb
       PCBs:   1-3 chloro  -  1.25 ppb
              4-6 chloro  -  2.50 ppb
              7-8 chloro  -  3.75 ppb
              9-10 chloro -  6.25 ppb

-------
            NDS PHASE II:  BIOACCUMULATIVE POLLUTANT STUDY
EPISODE ft:  3416
         SCC #:  DF025212
                   ERL-D Loc.:  1375
DATA for BIOSIGNIFICANT POLYCHLORINATED DIBENZODIOXINS AND FURANS:
Analyte               CAS No.:   Ion Ratio   S/N  %REC Det. Lim.   Amount(pgXg)
2,3,7,8- TCDF
2,3,6,7- TCDF
3,4,6,7- TCDF
51207-31-9
0.74
0.00
0.00
10.50
 1.00
 1.00
65
65
65
0.0000
0.5400
0.5400
2.16
                                              ND
                                              ND
2,3,7,8- TCDD
 1746-01-6   0.75
          1.00   86
               1.5400   ND
1,2,3,7,8- PeCDF
2,3,4,7,8- PeCDF
2,3,4,6,7- PeCDF
57117-41-6   0.00      1.00   88     0.7700   ND
57117-31-6   0.00      1.00   88     0.8500   ND
70648-29-9   0.00      1.00   88     0.8200   ND
1,2,3,7,8- PeCDD
40321-76-4   0.00
          1.00   86
               1.0900   ND
1,2,3,4,6,7- HxCDF *
1,2,3,4,7,8- HxCDF
1,2,3,6,7,8- HxCDF
2,3,4,6,7,8- HxCDF
1,2,3,7,8,9- HxCDF
1,2,3,4,7,8- HxCDD
1,2,3,6,7,8- HxCDD
1,2,3,7,8,9- HxCDD
1,2,3,4,6,7,8- HpCDF
1,2,3,4,7,8,9- HpCDF
19408-74-3
70648-26-9
57117-44-9
60851-34-5
72918-21-9
32598-13-3
57753-85-7
19408-74-3
67562-39-4
55673-89-7

0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00
0.00

1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00

85
85
85
85
80
80
80
56
56

2.8300
2.8400
1.9600
2.7700
2.4600
1.8400
1.3700
1.4400
2.6100

ND
ND
ND
ND
ND
ND
ND
ND
ND
1,2,3,4,6,7,8- HpCDD   37871-00-4   0.00
*:  Coelutes with 123478 HxCDF on DBS 30 H.
                       1.00   59
                        1.3200   ND
        Toxicity Equivalency Concentration:     0.22
        Percent contribution of 2378 TCDF,
        2378 TCDD and 12378 PeCDD to total
        TEF value:                               100

-------